LOCUS       DAOSAB010000001        14610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_3278_length_14610_cov_15.646994, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000001 DAOSAB010000000
VERSION     DAOSAB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 14610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14610
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3278_length_14610_cov_15.646994"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2368
                     /locus_tag="PK432_00005"
     CDS             <1..2368
                     /locus_tag="PK432_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOK59347.1"
                     /translation="ESTSSDLKNKINNIINETSTDAFDDLSIESETDISIISSNEQVN
                     KVEELLNKHGLTNEEYVSREGEYLNDDGKMCSIKAAKGLKLNNFTTGSRWEVVKDLSG
                     YPSHAQGGVDIKFDKNGVLFARSGKYIKAANGLVLSSKAVDSNKINTIPVSELYEQIT
                     GKPWSTAKEEGLTTGSYEDNIKLRQKLLSGEFNNKQSSNKIIQSKDFNQDVVDKIQKA
                     KTFKEAFRIARQQLGPNQIFEYKGRKYGTNLAGEEFNPSPETLAKHGMNKPEIIERLN
                     VQNKMVSSVYSDKTTTKLEPEYEDWDEIKKRKQEFNSMEQADRIITYKSKYESSNNRP
                     YVIIDKKKGILSIYIPGNKNPIFSTDKISLGAVISDAQTVTKVSDPNKDGIITKEEAA
                     VAKADFNAGNKSTGAGRYYISNVDKTGYKGLPIINMMNERQYDKYTQTGEVENVATSF
                     HIGYRPGYKRVSNGCIRCSENTLNILSTYLTPGSEVYILPEEEGNEFILSNGKLIFKS
                     NSKKDYNTYVDSYGNVQKGQGINTSVNTLNYIPIKIEIDKDKFVRDKHKWYDFDDNKE
                     FETVKKFVKALEENKRDIMEIAKIDGDLYNDIALVALGILGNETNFGDTHSETGNIFR
                     LINKGISTVNREYLGFKFLPSVTSSPDYKVKYNISKLLSVVSDDSIDDYSVGLTQLRF
                     KNIKVESEKQKEAIKAGKLPKNYVTLDKQLEQVGVTSAKDLMDPEKAAKATVIYLAYM
                     AKNRADVDINNVIETLPGKWGGSSSDNKQTYTNNAIKNMQYFKLKQLD"
     gene            2416..3636
                     /locus_tag="PK432_00010"
     CDS             2416..3636
                     /locus_tag="PK432_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59348.1"
                     /translation="MNKNIKVEAEGGELILRNEHGDYAIIPRKYRREVEDMIKEGCHG
                     CIDALVATLPGVNDYAEDGSVINVTSKEASDPDDPKPTSPVVETHKLPNGFNFVVYDD
                     TILKDFPDFKMSSGNFYIAKAVYDKLKRYDLSPEQMFAVIMYNRNVQEDFLLAKLKHY
                     ENKSIGRRVQEINDKRSKNIELNEDEKKLLKFVNYYNRVTSTGDPEEKQALIDRALQD
                     EDLRKTYFTKDNRFLFPDEDTATQLYATRDALEAFYSKNQTSKYINTTGTGIRDKDGN
                     LITTNSRDWGIRSTALIYPENDKFEINYPYVAREYVGNPTILANAIEGILLSKFDETN
                     DGLYYAIKAIRVDPRYWYEKERSKDPRHLWTSLDLNVEFPHYSGIAKALHKAINTFQD
                     NMAKNPEVYTLKVK"
     gene            3840..>7195
                     /locus_tag="PK432_00015"
     CDS             3840..>7195
                     /locus_tag="PK432_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59349.1"
                     /translation="MGDLEMFREYAAGVSVGYLEDKNHTVKIIIDTQVTPEFIDSINL
                     EDIIDINKYRQYAKVIISNFRERPPLFQTFNGAGVGAFTVHGNEWLYKHYNILNEKGK
                     LKQLSYKAASAWGSRLNKGHYTWKVVKLPSGYYSLVVTGVNENYKPETDLDISDHDLT
                     REQIMFLINSTSDFLIRDGDYIFGADRQKQIVDTVSALIYKQLISSKDGKRISVSKAF
                     ISAKNNLKSVANLYGKFLDIINKSQNRDKALEEIKTTLLETNQEKVFKALSAFNSAAA
                     LELAYNDIQRILIPEVWNQVQEHVISNLQNLSIYINREGNLENREQEFEDISDETVVD
                     ENDFSSEKNYSNESFHQDPKKTTTTNVKLFLSRIPSGKTSYLGLATYIPFDQVFDELK
                     RILSPITPEDIRTEDTPTLIDKYIKILTELGNKGKPYLLNIANVLSDPEVSVQFKNQF
                     IATMRVHENEFNMVLWEQDKNGNVNIRVINSNRNSAHFRVLEQWAESLKSSSLAIEKD
                     GRTILDTARIKKFKDRVINLNSKKNSTVAEYRELLSELFDLVGIVPSVTLTELLGEDN
                     YNKLIPNSKLNPKLSVNFEYSKSGYPKGVYSALINSLIKVGEQLEVNPDLTLDDINPY
                     NSKGFTDSSIITLAKIFARYSEILSTSSYRGSDGKTRYVFAYHAPATQRVERFNAENG
                     LLRKTLAKIPFSQRAFWLKFPNTKIELIDFEALKNARDREVVVMREDMEYREQDFTSA
                     SLFFASKHDSPTAMFFVPTMSDKKRTFIVKAPKIPVSIDESKVGFDEDSGNIIRKKGI
                     LTGEARTLIGDIVESELARMRALAEKKREVIANAETEQEKESALRKLKEEVGASFYEG
                     ANYFYLFPALNKFIYDKNGVFLEGVINDPEKIVLIKEAAILEVELEISSTISNWVKQG
                     FITYNKKTKNLETKIDVNYLKSLPAKTQAGKIVQAFADLTVNDYIAKASTIQVITGDP
                     ALAYNPKKFGSLPVEARVVATLDEYQKRGAKDVATGELGNTDWVINEKYRGFPFYNLI
                     ILDDDSHGTETEGFIKSIEEYKKKINRTDAQELTTVLEHINVLMNHGRISDEMYIRIR
                     DKIEKAGPEGYYELDDEELAAINQPVILQP"
     assembly_gap    7196..7295
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7442..13921
                     /locus_tag="PK432_00020"
     CDS             7442..13921
                     /locus_tag="PK432_00020"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK59350.1"
                     /translation="MEKNNVDRAVFSSGVKLGAGNVVSVFNSDGTIKDNISLSKENIH
                     TLDRKDFYIQQDVPYKEDKTEIRISSQMDKLVTTALVELAEEQFSLQVLNNPQFTSLS
                     GKELEILKQKVKTNLMMSAKRALYTRMGIKLRRDETGRVISIVEDVEKLATALRSSAK
                     KNGWSTNDLMHLKVENGRFVLSPGLGNAYEKIESLINAAITDTIKLHIRGASFVQGTS
                     SGFKSPKAKTLDELTSQEKSGIVWIKDIDNSFDPQKGLTYITRDEESGKITYAQILVP
                     WKYQADIKQFIDPETNTIDISKIDKELLNIIGFRIPNQGYSSQMVFQVVGFLPKSVGD
                     LAVVPGEVTTQMGSDFDVDKLYSYIYNHVVNDQGRLVKVPAFIDKDGNFDRQEAIKWI
                     NKNFATRRRNILTLKEKLPLIQESYNVSTTLINQFISEEEDKIEAEELDIRSKEVLEA
                     LGFTIEDITIPGIRRIETMSLENAYIGIHEAILGHSKLIDKILEPLDADDLKITAKNV
                     GRKISLDPYISRNRQLRDYSRQQAGKVGVSYFSRAVVGTATILKHKLQLVAGEEKNPV
                     YVTLFANDNGKVVPLNMISGNAVSYFDGDRSKPRTQLKNLVIMQSGSVDNAKDPVMDD
                     NNLNINTFPVAVAITLLSSESGEALDLRYVSYFLTQPAVKKIVNLIQSFSGSLSEDFI
                     AKSMIVPTSVGEVLNTLNQLYKEATGEELKEELYDKVKLSPKDMLEMIHEFDQKNPEH
                     IIKQCAIAVHFDKLYTIGQDILKVFRALSYESKGLPKNYWEIASFLEQVNSIRSITFQ
                     IDGVLDVLMQPQAKYVLDTTREAKTIIEQIMPVGSKVVTQTIEAISELYGRELTAEQK
                     RKIWLGIRSYIFSNPELFGVDEASNIDLLVGPNSLVKRIKRFLNTEAGKNFKLLSLLE
                     LKEAAKKENPDIIIYRSSASERVDEAMITTYFIDMLVSNNPEARTIAKDLIRYAYVTG
                     GNQAALQFVKYIPVAYLELIGFGDAVLSAFNSLEAEDSLIKQILQHTPKFAFRVSLEK
                     DSSKYNEKTGELRIKSENVVIGYGENTHPASYVSFYDSNSYTWRLFELDSYNGDTTTY
                     KEISLLGGKGKDYVAIDEYQYGNQHAVSVFESNVPYSVKKLISKSTNKEVSKPVSSGR
                     NATENNVNKVVSSIVNKVSKEVNDAFESLTAKIQALTSLTGNELVESLSKNVYSEYFK
                     TVLEVIANNKELLEGLKIKLINDTKSETVGSYDSSNHVLTINFWRIRHLSNENSALAV
                     KITERTITHELIHAVISRLVNAYMKNRNSVPKAIAEKLDRLEELRAYVESTLSEQEKI
                     ILQTAREKGVKAVSASELKPIYGLINLKEFLAETLVYDKFQQRLNNTPYNGHSILMEI
                     FQQIIGIFKELASKLGFDVKKNSALEAGLIEALELMQDINKFLTITPETKQDEIQQNQ
                     QSEKKEQKQEEQKPVNGLDENKPVTNTNNIFEFDNGVRVNTGHIILNKQQAKAVNLAA
                     AAIDNGQRKFVIRGYAGTGKSTVVGTILNYIKEKYKHQPRNIYGIANTHAAVLNLAAL
                     CHKQNVRTVTFKTIASFLTKIKVDGEFVPIALSRVQYNSIIIVDEASMISTSDYNDLI
                     DIANKKNLTLIFIGDKIQLPSPGNTKIDSNGDEYTEASPALEFQGDNQGIELTEVMRQ
                     SNESPLVEELHAVRENIGEEELPIGESTKINEKGEGILIFNNYREMLEKVVEAFRENP
                     HGVKYIAYTNKEVQAINEFVASRLGTLGFKKGAILTGYMTPAEAVIKNSSNYEVIEDP
                     KYVDEESVRLFTRVLPDGTYYDKTVKVSGYNVTVRLILPKSISEVLPVELKNTLSTPM
                     SIFIVDTKNQKNMEFFKEFARLKEIASDKKVSWTRRRDLSESLDNFYNKFQIPDNLIK
                     YKGNIYTIALLQQAHPELFVYDKEKRSRPVDELMKQGYIEKSIDYGYAITAHKSQGAT
                     FDVVFSSKKDLENPRANRKVMLGKEVVGLEKNMVAYVTFSRPSKVLALFNPDAKSTEN
                     TFREDQNKTTINGVGAKVQTNKQQPATKQEKQPSPEVNKASDLNEVDVFNAALEALGI
                     ETIDLGDITYTDEEGNICAEKGLRGKEFTKGSKWTIVKDLTGYPSHKEGGVDLKFDTG
                     GVKILRDGGEIIAKNGLVLPNVKRCKK"
     gene            13927..>14610
                     /locus_tag="PK432_00025"
     CDS             13927..>14610
                     /locus_tag="PK432_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59351.1"
                     /translation="MSCLPTYNGKRYNNIVELLKDNGVSQELIQKVSQLYKDYTASTK
                     NPSMDEFKNILKQKLSESKQQSVSIKPVPPLKYSTYVGNLKDGKFTSSDILPLEMFFA
                     VDAAGNLSYVGTLDDMKRLGDINSPAIKVVGGNVNTARDVVVVNYGEVKKVGNAWQVV
                     KPVEVVFTKDINEAIAESIPDTSTTKTISNTSESIKQKDSTGISKKSVSELFESNPEL
                     ANAVYEALGF"
BASE COUNT         5523 a         1995 c         2776 g         4216 t
ORIGIN      
        1 tgaatctact tctagtgatc tcaagaataa gataaataat attattaatg aaacttctac
       61 tgatgcattt gatgatctat caatagaatc tgaaacagat atatcaatta tttcaagtaa
      121 tgaacaggta aataaagtag aagaactgct aaataaacat ggccttacca atgaagaata
      181 tgtaagcaga gaaggggaat acctgaatga tgatgggaaa atgtgtagta taaaagcagc
      241 caaagggctt aagttaaaca acttcactac aggaagtagg tgggaagttg ttaaggacct
      301 ttctggatac cctagtcatg cgcagggtgg tgttgatatt aaatttgata aaaatggggt
      361 tttgtttgca agaagtggta agtatattaa agctgctaat ggattagttt tgtctagtaa
      421 agctgtagat tctaataaaa taaatactat acctgtttca gaattatatg agcaaataac
      481 aggtaaacca tggtctacag caaaagaaga aggattaaca acaggtagtt atgaagacaa
      541 cataaaactc agacaaaaat tactatctgg tgaatttaat aataaacaat cttcaaataa
      601 aattatacaa agtaaagatt ttaatcagga tgtagtggat aagatacaga aggcaaaaac
      661 gtttaaagaa gcatttagaa ttgctagaca acaattaggt ccaaatcaaa tatttgagta
      721 taagggaaga aagtacggaa ctaatttggc aggagaagaa tttaatcctt ctccagaaac
      781 acttgccaag catggtatga ataaacctga aataattgaa agactaaatg ttcaaaataa
      841 aatggtatct tcagtttatt ctgataaaac cacaaccaaa ttagagcctg aatatgaaga
      901 ttgggatgaa attaagaaga gaaagcaaga attcaattca atggaacaag ctgatagaat
      961 tataacttat aaatctaaat atgaatcttc taataatcgt ccttatgtta taattgataa
     1021 gaaaaagggt atattatcta tatatatacc tggaaataaa aaccctatat tttctactga
     1081 taaaatatct ttaggagcag ttataagtga tgcacagaca gttacaaaag tttcagatcc
     1141 taataaagat ggaataatta caaaagaaga agctgctgta gctaaggcag attttaatgc
     1201 tggtaataaa tctacgggtg ctggaagata ttatatatct aatgtagata aaacagggta
     1261 taaagggtta ccaataataa acatgatgaa tgaaagacag tatgacaaat atactcaaac
     1321 aggagaagtt gaaaatgtag caacatcatt tcatatagga tataggcctg gatataaacg
     1381 tgttagtaat ggatgtattc ggtgtagtga aaatacgctg aatatcctga gtacatactt
     1441 aacacctgga tctgaagtat atattttacc agaggaagaa ggaaatgagt tcatattatc
     1501 gaatgggaaa cttatcttta aatccaacag taagaaagac tacaatactt atgtagactc
     1561 ttatggaaat gttcaaaaag gtcaaggtat aaatacatcc gtaaatactt tgaattatat
     1621 accaataaaa attgaaatag ataaagataa atttgtaagg gataagcata aatggtatga
     1681 ctttgatgat aataaagaat ttgaaactgt taaaaaattt gttaaagcct tagaagagaa
     1741 taaaagagat attatggaaa tagctaagat tgatggggat ttatacaatg atattgcatt
     1801 agttgctctt ggaatattag gaaatgaaac taattttgga gacacacatt cagaaacagg
     1861 aaatatattt agattgatta ataaaggaat atcaactgtt aatagagaat atttaggatt
     1921 taaattttta cctagtgtaa catcatcacc agattacaaa gtaaagtata atataagtaa
     1981 acttttatct gtagtgtcgg atgatagtat tgatgattat agtgtaggat taacacagtt
     2041 aagatttaaa aatataaaag tagaatctga aaaacaaaaa gaagctatta aagcaggtaa
     2101 acttcctaaa aattatgtaa cactggataa acaattagaa caagttggag taacatctgc
     2161 taaagattta atggatccag agaaagcagc taaagctact gttatatatt tggcttatat
     2221 ggctaaaaat agagcagatg ttgatataaa taatgtaatt gaaacactac caggtaaatg
     2281 gggtggatcg tcgtctgata ataaacaaac atatactaat aatgctatta aaaatatgca
     2341 gtactttaaa cttaagcaac tagattaatg catgtataaa actgagtgac aataatttat
     2401 taataatgta tttgtatgaa taaaaatatt aaagtagaag cagaaggtgg ggagttaata
     2461 ctccgtaatg aacatgggga ttatgctatt atccccagaa agtatagaag agaggttgag
     2521 gatatgataa aggaggggtg tcatggatgt attgatgctc ttgttgcaac tcttccagga
     2581 gttaatgact atgctgagga tggaagtgtg ataaatgtaa cttctaaaga agctagtgat
     2641 ccagatgatc ctaaacccac aagtcccgtg gtagaaaccc acaagttacc taacggattt
     2701 aattttgtag tatatgatga tacgatttta aaagactttc cagacttcaa aatgtcctct
     2761 ggaaacttct acatagctaa ggctgtatac gataaattga aaaggtatga tttgtctcct
     2821 gaacaaatgt ttgcagtaat aatgtataat agaaatgttc aagaggattt tctattggct
     2881 aaacttaaac attatgaaaa taaatcaatt ggcaggaggg ttcaagaaat taatgacaaa
     2941 agaagcaaga atattgaact aaatgaggat gaaaagaaat tattaaagtt tgtaaattac
     3001 tacaacaggg taacttcaac aggtgatcca gaagaaaaac aagcactaat tgatagggca
     3061 ttacaggatg aagatttaag aaaaacctac tttacaaagg ataatagatt tttatttcca
     3121 gatgaagata ctgctactca actatatgct actagagatg ctctagaggc attctacagt
     3181 aaaaatcaaa cttctaagta tatcaataca actggaactg gaattcgtga taaagatggt
     3241 aatttaatta caacaaattc tagagattgg gggataagga gcactgctct gatttatcca
     3301 gaaaatgata agtttgaaat aaactaccca tatgttgcta gagagtatgt aggtaatccc
     3361 accatactag ccaatgcaat agaaggaata ttactaagca agtttgatga aactaacgat
     3421 ggattatact atgcaattaa ggctataagg gttgatccta ggtattggta tgaaaaggaa
     3481 cgttctaagg atcctagaca tctatggaca agtttggatt taaatgtaga atttccacat
     3541 tactctggaa tagctaaagc cttacataaa gctataaata cctttcagga taacatggct
     3601 aaaaaccccg aagtttatac tttaaaagta aagtaggtat aacaaaaaag ttatgcctat
     3661 tctacatagt aaaatttatt tcttactata tttgcatact ataacaaatt aatctaataa
     3721 ctatgtcttg cagacccata agagatagag ttagagaagc tggagcacat cttctctcca
     3781 atcctaattt tataaaaaaa gcagatcagt tttttaattc ccatgctgct ctcaaatata
     3841 tgggagacct tgaaatgttc agagagtacg ctgcaggagt atctgtggga tacctagagg
     3901 acaaaaatca tacagttaaa ataattatag atactcaagt aactcctgag tttattgaca
     3961 gtattaatct tgaagatata atagacatca acaaatatag gcagtatgct aaagtaatca
     4021 tatctaattt cagggaaaga cctcctctat ttcaaacatt taatggtgct ggagttgggg
     4081 cttttacagt gcatggcaat gaatggctat ataagcacta caatattctc aatgagaagg
     4141 gcaagttaaa acaactttca tataaagctg cctctgcttg gggaagtagg ttaaataagg
     4201 gacactatac ctggaaagta gttaaattgc cctcagggta ttattcttta gttgtcactg
     4261 gtgtaaatga aaattataaa ccagaaacag atttagacat ttcagaccat gatttaacta
     4321 gggaacaaat aatgttcctc ataaactcta caagtgattt tcttattagg gatggggatt
     4381 atatttttgg agctgataga cagaagcaaa ttgttgacac tgtttcagcc ctaatatata
     4441 aacaacttat ttcctctaaa gatggtaaaa gaatttcagt atctaaagca tttatctctg
     4501 ctaaaaataa tttaaaaagt gttgcaaatc tctatggaaa gtttcttgat attataaata
     4561 aaagccaaaa tagggataaa gctttagaag aaattaaaac taccctactt gagactaatc
     4621 aagaaaaagt ttttaaagcc cttagtgctt ttaatagtgc agcagctcta gaacttgcat
     4681 ataacgatat tcaaagaatc cttatacctg aagtttggaa tcaggttcag gagcatgtaa
     4741 tttcaaatct tcagaacctt tctatttata ttaataggga aggtaacctt gaaaatagag
     4801 agcaggaatt tgaagatata agtgatgaaa ctgtagtaga tgaaaatgat tttagtagtg
     4861 aaaaaaacta ctctaatgag tcatttcatc aagaccccaa gaaaactact accactaatg
     4921 taaaactgtt cttatctcgc ataccttcag gaaaaacttc ctatctagga ttagctacct
     4981 atattccatt tgatcaagta tttgatgaac ttaaaagaat actaagccca ataacacctg
     5041 aagatataag aacagaagat actccaaccc taattgataa gtatataaag attttaactg
     5101 agttaggtaa taaggggaaa ccctatctac tgaatattgc taatgtacta agtgatcctg
     5161 aggttagtgt gcagtttaag aaccaattca tcgccacaat gcgggttcat gagaatgaat
     5221 ttaatatggt tctatgggaa caagataaga atggaaatgt aaatattagg gtaattaatt
     5281 caaatagaaa cagtgcacat ttcagggtac ttgaacaatg ggcagaaagt ttaaaaagta
     5341 gttctttggc tattgaaaag gatggaagaa ccatattaga cactgcaaga ataaaaaagt
     5401 ttaaagatag ggttattaat ctcaactcaa agaagaattc aactgttgct gagtatagag
     5461 agttactgag tgaactgttt gatttagttg gtatagttcc tagtgtaact ctaacagaat
     5521 tgcttgggga ggataactac aataaactta ttcctaatag taaattaaat cccaaattaa
     5581 gtgtaaattt tgaatacagc aaatctggat atcccaaggg agtatattct gctcttataa
     5641 actcacttat caaagtggga gagcaactag aagttaatcc agatctaact ttggatgata
     5701 tcaatcctta caatagtaag ggatttacag attcatccat tataacttta gctaaaatat
     5761 ttgctaggta ttcagaaata ctctccactt cttcatatcg tggttctgat ggtaagaccc
     5821 gatatgtgtt tgcataccat gctcctgcta ctcagcgtgt agaaagattt aatgcagaga
     5881 atggattact gagaaaaact ctagctaaaa ttccattctc tcaaagagcc ttttggctaa
     5941 aatttccaaa tacaaagatt gaacttatag actttgaagc tctaaaaaat gctagggata
     6001 gagaagtggt ggttatgcgt gaggatatgg agtataggga acaggacttt acctctgctt
     6061 ccctattctt tgcatctaag cacgactccc ctactgctat gttttttgtt cccacaatgt
     6121 ctgataagaa aagaacattt attgtaaagg ctcctaaaat acccgttagt attgatgagt
     6181 ctaaggttgg atttgatgaa gatagtggaa atattataag gaagaaggga atacttacag
     6241 gagaagcaag aactcttatt ggggatattg ttgaatctga acttgctaga atgagagctc
     6301 ttgctgagaa gaaaagagag gtaatagcaa atgctgaaac agaacaagaa aaggagagtg
     6361 ctctaagaaa attaaaagaa gaagtggggg cttcatttta tgaaggagcc aactatttct
     6421 acctattccc tgcacttaat aagtttatct atgataagaa tggtgttttc ttagaaggag
     6481 ttattaatga tcctgaaaaa atagtgttga ttaaggaagc agccattcta gaagtagaac
     6541 tagaaatatc ttccacaata agtaattggg ttaaacaagg tttcataaca tacaacaaaa
     6601 aaactaagaa ccttgagact aaaattgatg taaactattt aaagtcctta cctgcaaaaa
     6661 cacaggcagg taagatagta caagcatttg ctgatttaac tgttaatgat tacatagcaa
     6721 aggcaagtac aatacaggta attactggag accctgcatt agcatataac ccgaagaaat
     6781 ttggatcact accagtagaa gctagagttg ttgcaacact tgatgagtat caaaaacgtg
     6841 gtgctaagga tgtagcaact ggtgaattag gtaatacgga ttgggttatt aatgaaaaat
     6901 ataggggatt tccattctat aacttaataa ttcttgatga tgattctcat gggactgaga
     6961 cagaaggatt tattaaaagt atagaagaat ataaaaagaa aataaataga accgatgctc
     7021 aggaacttac cactgtgcta gagcatatca atgttcttat gaatcacggt aggatttctg
     7081 atgaaatgta tatccgtatt agggataaaa ttgaaaaggc aggaccagaa ggctactatg
     7141 agttggatga tgaagaactt gcagctatta atcagcctgt aatcctgcag cctatnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntaatc ctgcagccta ttaaaccagt
     7321 tcaggttcac tttaaagagc agtctggttt atatgagaga atttactacg ttaaaacctc
     7381 atcctaccct cttattcctc aattaactgc tggaatggaa attgataagt tgaggcggtt
     7441 aatggaaaaa aataatgttg atagggctgt ttttagttca ggtgtaaaac ttggagctgg
     7501 aaatgttgtt agtgtattta attctgatgg aactattaag gataatataa gccttagtaa
     7561 ggaaaacatt catacactag atagaaaaga tttctatata cagcaagatg ttccatataa
     7621 ggaagataaa acagaaatac gtatttccag tcaaatggat aaactggtaa caacagcttt
     7681 agtggaactt gcagaagaac agttttcact tcaagttctt aataacccac agtttacttc
     7741 cttaagtggc aaagagttag aaatcttgaa gcagaaggtt aaaactaacc taatgatgtc
     7801 tgctaagaga gctttatata ccagaatggg tattaagtta agaagagatg aaacaggtag
     7861 ggtgatatcc attgtagaag atgtagagaa attagcaact gctcttagaa gttctgcaaa
     7921 aaagaatggt tggagtacta atgatttaat gcatttaaaa gttgaaaatg gtaggtttgt
     7981 actatcccca gggctaggaa atgcctatga gaaaattgag tccttgataa atgcagctat
     8041 tactgatact attaagttac atattagggg agcttcattc gttcaaggta cttcttctgg
     8101 atttaagtct cctaaagcaa aaactttaga tgagcttaca agtcaggaaa agagtggaat
     8161 agtttggata aaagatatag ataatagttt tgacccacaa aagggattaa cctatattac
     8221 tagagacgaa gagtctggaa agattaccta tgcacaaatt ctagtaccct ggaaatacca
     8281 agcagacatc aaacaattca tagaccctga aaccaatacc atagatattt caaaaataga
     8341 taaagaactt ttaaatatta ttggtttccg tattcctaac caagggtata gttcccaaat
     8401 ggtgtttcaa gttgtaggat ttctaccaaa aagtgtggga gatttagcag tagttccagg
     8461 agaagtaact actcagatgg gttctgactt tgacgtagat aaactataca gttacattta
     8521 taaccatgtt gttaatgatc agggtagatt agttaaagtt cctgcattca ttgataaaga
     8581 tggaaacttt gataggcaag aagctataaa atggattaat aaaaactttg ctactagaag
     8641 aagaaatatt ttaactttaa aggaaaaact tccattaatc caagaaagtt acaatgtaag
     8701 tacaacactt attaatcagt ttataagtga agaggaagat aaaatagaag cagaagaatt
     8761 agatataaga agtaaggaag ttctggaagc tcttggattt accattgaag atattactat
     8821 tcctggaata cgaagaatag aaactatgtc attagagaat gcttatattg gtatccatga
     8881 agcaatactt gggcattcaa agttaataga taagatacta gaaccacttg atgcagatga
     8941 tttaaaaatt actgctaaga atgttggaag aaaaataagt ctagacccat atatttcaag
     9001 aaacagacaa ttaagagatt acagcagaca gcaggctggt aaagttggag tttcttattt
     9061 ctctcgtgca gtagttggta ctgctacgat tttaaaacat aaattacagt tagttgctgg
     9121 agaagaaaaa aatccagtat atgtgacact ttttgctaat gataatggaa aggtagttcc
     9181 actaaatatg attagtggca atgcagttag ttactttgat ggagataggt ctaaacctag
     9241 aactcaatta aagaaccttg ttattatgca aagtgggtct gtagataatg ctaaagaccc
     9301 tgtaatggat gacaataact taaatattaa tactttccca gtagctgtag ctattaccct
     9361 tttaagcagt gaaagtgggg aagcattgga cctaagatat gtttcctact ttttaacaca
     9421 acccgcagta aagaaaatag ttaatttaat acaatcattt tctggtagcc ttagtgagga
     9481 ctttattgca aagagtatga tagttccaac aagtgttgga gaagtcttga atacccttaa
     9541 ccaattatac aaagaagcta ctggagaaga actaaaagaa gagttgtatg ataaagtaaa
     9601 gctgtctcct aaagacatgc ttgaaatgat tcatgaattt gaccaaaaaa atccagaaca
     9661 cattattaaa caatgtgcta tagcagttca ttttgataaa ctatatacta ttggacagga
     9721 tatattaaag gtatttagag cactttctta tgaaagtaaa ggactaccaa agaattactg
     9781 ggagattgct tctttcttag aacaagtaaa ttctataaga agcattacat ttcaaattga
     9841 tggagttctg gatgtgttaa tgcagccaca agctaaatat gttttggata caactagaga
     9901 agccaaaact ataatagagc aaattatgcc tgtgggaagt aaagtggtaa cacaaacaat
     9961 agaagccatc tcagaactat atggaagaga gttaactgca gaacaaaaaa gaaagatttg
    10021 gttaggcatt agatcttaca tattctctaa tccagagcta ttcggagtag atgaagccag
    10081 taacattgat ctacttgttg gacccaatag tttggttaag agaattaaaa ggtttttaaa
    10141 tactgaagca ggtaaaaact ttaaactact aagtttacta gaacttaaag aagcagccaa
    10201 aaaagaaaat cctgatataa tcatctatag aagttcagcc tctgagcgag tagatgaggc
    10261 tatgattact acctacttta ttgacatgtt agtaagcaac aatcctgaag caaggacgat
    10321 tgctaaagat ttaatacggt atgcttatgt aacaggtgga aatcaggctg cccttcagtt
    10381 tgtgaagtat attccagtag cctatcttga attaattggt tttggggatg cagttctttc
    10441 tgctttcaat agtctagagg ctgaagatag tttaattaaa cagatactac agcatactcc
    10501 aaaatttgca tttagggtat ccctagaaaa agatagcagt aagtataatg aaaagactgg
    10561 ggaacttaga ataaaaagtg agaatgttgt aattggttat ggtgaaaata cacatccagc
    10621 ttcttacgtg tctttctacg attcaaacag ttatacatgg agattgtttg agttagatag
    10681 ttataatgga gataccacca cttataaaga aatttcgtta ctaggtggta aggggaaaga
    10741 ctatgttgca atagatgagt accaatatgg aaatcaacat gcagtatctg tatttgaaag
    10801 taatgttcca tatagtgtaa agaaattaat ttcaaaatct actaacaaag aagttagtaa
    10861 accagtttct agtggaagaa atgctacaga gaacaatgta aataaagttg tttcaagtat
    10921 tgttaataaa gtttctaaag aggttaatga tgcatttgaa agtttaactg ccaaaattca
    10981 agccttaact agtttaactg gcaatgagtt ggtagaatcc ctgtcgaaga atgtttattc
    11041 cgaatacttt aaaactgttt tagaggttat agcaaacaat aaagaattgc tagaaggatt
    11101 gaagattaaa cttataaatg atactaaatc tgaaactgta ggttcatatg attctagtaa
    11161 tcatgtttta acaattaact tttggagaat tagacattta tccaatgaaa attctgcact
    11221 agcagtaaaa attacggaaa gaactattac acatgaacta attcatgctg taatctctag
    11281 actagtaaat gcatacatga agaataggaa tagtgttcca aaagctattg ctgaaaaact
    11341 tgataggtta gaagaactta gggcttatgt agaatctact ttgagtgaac aagaaaaaat
    11401 tatactacaa actgctagag aaaaaggggt aaaggcagtt tctgcatctg aactaaaacc
    11461 aatttatgga ttaattaacc taaaagaatt cttagcagaa acacttgtat atgataaatt
    11521 ccagcaaaga ctaaataata ctccttacaa tggacatagt attctaatgg aaatattcca
    11581 acagattatt ggcatattca aagagcttgc ttcaaaatta ggatttgatg ttaaaaagaa
    11641 ttctgcactt gaagctggat tgatagaagc attagaatta atgcaggata ttaataaatt
    11701 tttgactata acaccagaga ctaaacagga tgaaattcag caaaatcaac agagtgagaa
    11761 gaaagaacaa aaacaagaag aacagaaacc tgttaatggt ttagatgaaa ataaaccagt
    11821 aactaataca aataatattt ttgaatttga taatggagtt agggttaaca ctggtcatat
    11881 tattctgaat aaacaacagg ctaaagctgt taatcttgca gcagctgcaa ttgataatgg
    11941 tcagagaaaa tttgttatta gaggatatgc tggtactggt aagtctacag tagtaggaac
    12001 tatacttaat tatattaagg aaaaatacaa gcatcagcct agaaatattt atggtattgc
    12061 caatacccat gctgcagttc tcaatcttgc agcactatgt cataagcaaa atgtacgaac
    12121 agttacattc aaaaccatag caagtttcct tactaagatt aaggtagatg gagagtttgt
    12181 acctattgcg ttaagtaggg ttcaatataa ctctataata attgttgacg aagcaagtat
    12241 gatatcaaca agtgactaca atgatttaat tgatatagct aataaaaaga atctaaccct
    12301 gatatttata ggagataaaa ttcagttacc ctcacctgga aatactaaaa ttgacagcaa
    12361 tggtgatgaa tatacagaag ctagcccagc acttgaattt caaggtgata atcagggaat
    12421 agaactaact gaggttatga gacagtccaa tgaaagtcca ttggttgaag aattacatgc
    12481 agtgagagag aatataggag aagaggagct tccaataggg gaatccacca aaataaatga
    12541 aaagggggaa ggaattttaa tattcaataa ttatagggaa atgttggaga aagtagtgga
    12601 agcatttaga gaaaatccac atggagttaa atacatagcc tatactaata aagaggttca
    12661 agctataaat gaatttgtag ctagcaggct tggaactctt ggatttaaga aaggagccat
    12721 acttactggg tacatgactc cagcagaagc agttattaaa aattcttcta actatgaagt
    12781 tatagaagac cctaaatatg tagatgaaga gagtgttagg ttatttacta gagtacttcc
    12841 tgatggaacc tactatgata aaacagttaa ggttagtggc tataatgtaa cagtaagact
    12901 aatacttcca aaatctatat cagaagtttt acctgtggaa ttaaagaata ccctatcaac
    12961 tcctatgtct attttcatag tagatacaaa aaatcaaaag aatatggaat tctttaagga
    13021 gtttgcacgt ttaaaggaaa ttgcttctga taaaaaagtt tcatggactc gtagaagaga
    13081 tttaagtgaa agtttagaca atttctataa taaattccaa atacctgata accttataaa
    13141 atacaaagga aatatctaca caatagcatt actacaacag gcacatcccg aactgtttgt
    13201 atatgataag gaaaaaagaa gtaggcctgt tgatgagtta atgaagcagg ggtacataga
    13261 aaagagcatt gactatgggt atgctataac agctcataag tctcaaggtg caacctttga
    13321 tgtggtattc tcctctaaga aggatttgga aaatccaagg gctaacagaa aggttatgct
    13381 tggtaaggaa gttgtaggac tagaaaagaa tatggtagcc tatgttacat tctctagacc
    13441 ctctaaggta ttagcactgt ttaatcctga tgcaaagtct actgagaaca catttagaga
    13501 agatcagaat aagactacta ttaatggtgt aggagctaaa gtacagacta ataaacagca
    13561 acctgctacc aaacaggaaa aacaaccatc tccagaagtt aataaagcct ctgaccttaa
    13621 tgaggtagat gtatttaatg ctgcactgga agccttggga atagagacta ttgatcttgg
    13681 agatattacc tacactgatg aagagggtaa catatgtgct gaaaagggat taagaggtaa
    13741 agaatttact aagggttcta agtggaccat agttaaagac ttaacaggat atccttcgca
    13801 taaagaaggt ggggtagacc taaaatttga tacaggtggt gttaaaattt taagggatgg
    13861 tggagagata attgctaaaa atggattagt tttgcctaat gtcaaacgtt gtaaaaaata
    13921 aatagtatga gttgtttacc tacttataat ggaaaaagat ataacaacat tgtggaactt
    13981 cttaaggata atggagtttc ccaggaactt atacagaaag tgtcacaact ctataaggac
    14041 tatactgcgt ccacaaaaaa tccatcaatg gatgagttta agaatattct taaacagaaa
    14101 ctgagtgaat ccaagcagca atcagttagt attaaacctg ttcctccact taaatattct
    14161 acctatgtag gtaaccttaa ggatggaaag tttaccagca gtgatattct acctttggag
    14221 atgttttttg cagtagatgc tgctggaaat ttatcctatg taggtactct agatgatatg
    14281 aagaggcttg gggatataaa ctctcctgca ataaaagtag ttggtggtaa tgtaaacaca
    14341 gcacgggatg ttgtggtggt aaactatgga gaagtaaaaa aggtaggtaa tgcatggcag
    14401 gtagttaaac ccgtagaagt ggtattcact aaagatatta atgaggcaat agccgaaagt
    14461 attcctgata ctagtactac taaaactatt agtaacacat ctgaatcaat taaacaaaaa
    14521 gattctacag gaattagcaa aaaatctgtt tcagaactat ttgaatccaa tcctgaatta
    14581 gctaatgctg tatatgaggc tttagggttt
//
LOCUS       DAOSAB010000002        13045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_3741_length_13045_cov_13.787067, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000002 DAOSAB010000000
VERSION     DAOSAB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 13045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13045
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3741_length_13045_cov_13.787067"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..645
                     /locus_tag="PK432_00030"
     CDS             136..645
                     /locus_tag="PK432_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOK59352.1"
                     /translation="MKKIIWILTLILPLCAGAPNIDSKLISLVRIEARINAYIDNYLK
                     LQKRIESLLFAIKMVESNGNYFSIGNSGEFGAYQFTLKTWESLCLKYVGKELDIRIPD
                     NQDYIAFLKVKSLIEKGYSDEEIASIWNSGSSVWHRKVGINKFGIPYDVPKYVNKIVK
                     LKNEIHNKL"
     gene            657..965
                     /locus_tag="PK432_00035"
     CDS             657..965
                     /locus_tag="PK432_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59353.1"
                     /translation="MKMRIEFLNDNGNVVKTIDSDYKTFIEFIENIHYLTELRKNITS
                     ISKELESTLWYIKFLCDDLGIDMQDIAKHKRILSMLEQRNKSNVDKNNKQNESTKFMF
                     "
     gene            977..1303
                     /locus_tag="PK432_00040"
     CDS             977..1303
                     /locus_tag="PK432_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008882255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HOK59354.1"
                     /translation="MNFNEYQQKIKVTAQYPPSLRVIYPALGLGGESGEVLEKIKKVY
                     RDKNGVFTKEDIDSISKELGDVLWYVQALCNDLGISMQDVAEQNVTKLSNRLNNNTIH
                     GNGDDR"
     gene            1323..2018
                     /locus_tag="PK432_00045"
     CDS             1323..2018
                     /locus_tag="PK432_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK59355.1"
                     /translation="MAITVGIFQQSGGGKTMSIVVGPDGTYDIKGEYKGMNPSTTVYI
                     NSDRKFPPFKLDNWKEGVNMFTTSDVEIIMDLLQKINNGTKIKSVIIDTINGIMIDKE
                     MLESKKLTYDKWMDLAKDIYEIITFANSMRNDLIIYFMGHVTLYTDVDGNESKCLVTN
                     GRKLEKIKVESKLPVVLFGAVERGLNGDNKYYFETQSNRSTAKTPPGMFEDFKIPNSL
                     VYVDTKIREYYGI"
     gene            2019..2294
                     /locus_tag="PK432_00050"
     CDS             2019..2294
                     /locus_tag="PK432_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59356.1"
                     /translation="MFNLNLLVMVKIVVDLFKSIAKFIGLVFMIISVVPWKLGQYLLS
                     IKTSKEKELEQILMGKTRTSKKQPVKQERKKREKRSNTNKMHTTTVE"
     assembly_gap    2345..2444
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2513..3328
                     /locus_tag="PK432_00055"
     CDS             2513..3328
                     /locus_tag="PK432_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59357.1"
                     /translation="MPAININNSSKEVLKQSVVYTGLTQVKMVAVNPTLKELQEMGYN
                     FQQEPVYLTEEEGVRKLRLDFYFKNATVSNKITFFLENRPRVNKDGTKNEFINHYGQS
                     TWAADLNEALNKTASNGKKWFNAEGAREAYVGETSLIEFLKQWLNTNPNDKVYIEDME
                     ALFKGNVKELQYYVNAAKDNVVWVLLTYSEKEGKYYQNVDNGLFIRGYFKQVAAFSKA
                     VEYIKRKEKDGYPVKGYNGLEFKEFVPPAPEVILPDSDLDMLKGKIDDGSNVF"
     gene            3391..4422
                     /locus_tag="PK432_00060"
     CDS             3391..4422
                     /locus_tag="PK432_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59358.1"
                     /translation="MIPIRKPLTKENILSLISSYDIFKFYSPNFQKLNKLFKSDLRKE
                     NNPSCSITSINGDLLYTDFGLGKSFRCIDFVMFKYNLTYAEALQKINMDFNLGLGGNI
                     TLDTSTSQSPIIHEKINIQDKPPTIIQKKKRPFTKKDLQYWNEFYWTEEMLKLSKTES
                     ISHYWINGNMITVGDELAFSYEYYWHNGRFQRKLYFPERSQYKWISNVDNTIVQLVDV
                     MPKYGDILFITSSKKDAGIFWRMNMEGMFRNIVVHGVAPNNEVSFVPKEWFRKIKTRY
                     KSIIIWYNNDFTGINNASKFSSEYEIPYCHNPIGSPKDPSDFCKEYGIKKFFNFVTDN
                     LIKLELYEP"
     gene            4412..4846
                     /locus_tag="PK432_00065"
     CDS             4412..4846
                     /locus_tag="PK432_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59359.1"
                     /translation="MNPKIEIFIPGNIPSLKNSKINGRFPSKTVTKWLREFGIVWYSS
                     RDKKVKEFKRIPKKYSFAEICNPIKECREYPLHLGFFFIRNSRRKWDFHNACQIVLDL
                     MTVYDIIPDDNVDFVLPFPLEIEGKYWDLNKENPGVIIKVLN"
     gene            4873..5571
                     /locus_tag="PK432_00070"
     CDS             4873..5571
                     /locus_tag="PK432_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59360.1"
                     /translation="MSQVERLSAKRKVSFKLPATVQLISIESDAKTFGQLMEDVANSV
                     IADKIPTHSERVDNNYVRTYKFIEKNSLAEYGKLDDAVLPEGNIILFVTPVDHKAGLT
                     INFEMNWPLERKIEAINNMSYNEIRSLGYILNQEHNAGISLSGSKREVINNIIEFLKT
                     RESTPNFVEELELLKKDLQFLKEALFTNSVTVDLMIDKVNNLIEANSNKDSTEEDLGL
                     LDIHNNIMEKFKEL"
     gene            5648..6916
                     /locus_tag="PK432_00075"
     CDS             5648..6916
                     /locus_tag="PK432_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59361.1"
                     /translation="MERLKIINKLVLGHNTLLLQLQKLSDEEREKIFEYSKFYRPESL
                     VLIPDSYGSNSFILGKIISRTHKKIVILADSGIIEIFGLNSIDKSVIALNKENYHDLY
                     KLKLNQLSPKKLQKVVESIVKSINIIHDYEIIQISSTEISVNLYLPEIEIKNSLEMSH
                     IMRECYIKFTLVFTNNKIEVRNLQFYRANFTPSEVSADYLFSHSNTHPGEVARDFCFG
                     GSELREYMRGERFYVNDLKYVIYLILHYFSWESLEGVPYRRIENILPYKIETINDSSL
                     TDDEDFKILLKSLIDNPIDLSINFNDFYSDLSSDLSVDEEFRNKLGERIQELFGEKYM
                     TYKLGEMEGKLISKTKETVKLIYNQTFKFKNQVIKPIIEESEYEKKILESALAQCTKV
                     VRRDTINYVCSVLYLDYILQNLLASSLFTN"
     gene            6930..8084
                     /locus_tag="PK432_00080"
     CDS             6930..8084
                     /locus_tag="PK432_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59362.1"
                     /translation="MNKIVISKLLKQQIDYFHTKIKNTEWSGILLFDVVKGNFKKLED
                     IEIYAKSMHLMNIGNSTYTEFTVDADLIKAYNNINDHELKSLNGFIHTHHTMNAFFSG
                     TDLKELEDNADKYNFFVSLVVNMEGEYVCKIAIPTKTITKKTIIFKDHQENIVKRNTE
                     EESTEILYIDLKPVFEEEVSVPKWVEENYLEVKNKKTENFQNTYFKNNDYYVSNHTER
                     TSNSKDYLPSSNPNKIYKWPNIPNSYTPDPYKSTLYRSDSYSKTDKETCMNSSNGEYT
                     KCLLWVKAILNADDNADLKELEKRNVGDILKDLIKSTSKDLEMYEMYVDLLEPIIEQN
                     YEEIFSDDPTFLKYPTRADIAIKIINNIPGIDLNHYLVELIIDTLKIGGN"
     gene            8086..8778
                     /locus_tag="PK432_00085"
     CDS             8086..8778
                     /locus_tag="PK432_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOK59363.1"
                     /translation="MGYYERFKDAPWYEQSANEYITIIGAGGIGSNTAFNLIRTLKSK
                     IEILDFDMVEEHNIGTQFYLTNQINSSKVSSLKYTMGLFDPTSYSRVRYIQAKLSDYN
                     GVYPITICAVDNMDARKEAFELWKNLENRELFIDGRLTANIYQVFTVTPDKEELYAKS
                     LFPNSMIEDGPCTFKQTSYFASMIGARITQMVVNYLINKYAENTICNLPFAIMESSEL
                     VLFKTASSYDNL"
     gene            8876..9838
                     /locus_tag="PK432_00090"
     CDS             8876..9838
                     /locus_tag="PK432_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59364.1"
                     /translation="MEILRLKDLGKHYFDSLDPSKGIIRISTSNIFINIIVFAGMVNI
                     ANDYGNSMNMIIVKNRNGKRVGYVKTKIVNNSGLFSNSTFQLLKRIARFLGVKEVIIN
                     NYEYNKYTIVSKLNANITRNNGKSEKLTAVNQEYLIKVYDSMVFDNLLTNIAGEHSNI
                     YSTDNGIPENTDIHVLSTLVKKFFNFNYDFYINTRDNNNIFYMVFRKKNSWIISKNMD
                     HEVEPLYVIGSVGYKLMTVNRDSKVELLGPEELIFFNADMWNDWNVNDQTIFISKLDE
                     TLNNSDFYFSRWLNYHNNETFFKNGTLIKALDLVSSTIVNTNLS"
     gene            9959..10699
                     /locus_tag="PK432_00095"
     CDS             9959..10699
                     /locus_tag="PK432_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59365.1"
                     /translation="MEKTKCTHYALFVSTGGNYFRKKVADSVYKKNRDKYQIRQWTKT
                     LKAILEDRYHANNCVGVEADDLVLYWYNKPLFFNGNSIVDSVEPSSNVEEVTKILCSI
                     DKDLLKTVPGKHFNYTFKTNPENNETIMGQWIETTEEEAEEFLLKQLVTGDSTDNIIG
                     LPGKGEVFWNKWKKAQSDKISIGNILDLYCKEIYSKSKAIKTFYNNFTMLYMLRTPED
                     FEANGLELPSFPFIHAVPNNQMLIDVEF"
     gene            10721..10972
                     /locus_tag="PK432_00100"
     CDS             10721..10972
                     /locus_tag="PK432_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59366.1"
                     /translation="MKSKTFNVKIDVLKKIRSRVEIVVEADSISEVYHRIKNLAYVSD
                     FKKKGVTLLSEKDSPDYSLEMISLRKVEELPVQTRLDFD"
     gene            11093..11779
                     /locus_tag="PK432_00105"
     CDS             11093..11779
                     /locus_tag="PK432_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59367.1"
                     /translation="MENIEEETNRILSVSVGDFAIKIGDYFQHEREEIPSSYYVEEIM
                     RGGSKPHFKRAYTIRSYKRNKGTNYILPCLGNDYLFFDTNDYLINSYLNIDFTRLYLL
                     YRFSKTEYYSKIESRLVNHPRFVTTDSSIPGFDIFIFNIPEEHQEDAKLFMKGKYSKL
                     SDNLKFDIKSFYKLTNNSFTWKVLTKDPKLIAQMETKFGLTKIFHSIDLDEIPDPNEE
                     IWERFIKNFK"
     gene            12004..12246
                     /locus_tag="PK432_00110"
     CDS             12004..12246
                     /locus_tag="PK432_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59368.1"
                     /translation="MKDVLKKWLSNGVIYDSIGIQIYIPENNAYAKHPIPLLNIQGES
                     YIKNFLGLNDEDVLKFQDELGKFIAEAINEKIARSQ"
     gene            12348..13010
                     /locus_tag="PK432_00115"
     CDS             12348..13010
                     /locus_tag="PK432_00115"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOK59369.1"
                     /translation="MEPFIMDYLGNNINQDLFLQIGRKIASIRKTRVVYPNSSEVFRA
                     FKETPIQHLKVVLLFQDPYHNGSADGLATSCSRTGISTTLQIMLREIDLEYPEWINDL
                     NYGREDRKNLSRWAKQGVLLLNTALTVEKGKPESHTKIWEPITTAVVKTINSKQDIVW
                     GLFGTRAHYYKKFITNPTHTVFKVAHPAAEAYGSKGFIGCGVFRKINEELEARNKSII
                     QW"
BASE COUNT         5113 a         1605 c         2187 g         4040 t
ORIGIN      
        1 aattcataaa gtttttgaag cgctcaagaa tatggttaaa tgtattttta ggctatgatt
       61 ttgacacgtt taggaaaatt aacactttta tatcattaaa atcagtatat ttgcatttac
      121 ctaaatttat acagtatgaa gaaaataatt tggattttaa cattaatact tccattatgt
      181 gcaggagctc ctaacattga tagcaaatta atttcactag taagaataga agcaaggata
      241 aatgcatata tagacaacta tttgaaatta caaaagcgta tagaatccct gctttttgca
      301 ataaaaatgg tagaaagtaa tggaaattac ttttcaatag gaaattctgg ggaatttgga
      361 gcgtatcaat ttactttaaa aacctgggaa tccttatgtc taaaatatgt tggaaaagaa
      421 ttggatatta gaatacctga taatcaagat tatatagctt ttctaaaagt aaaaagtctg
      481 atagaaaaag ggtattcaga tgaagaaatt gcttctatat ggaatagtgg aagtagtgta
      541 tggcacagaa aagttggaat aaacaaattt ggtattccat atgatgtgcc taaatatgtc
      601 aataaaatag ttaaactaaa gaatgaaatt cacaacaaac tttaaagcct gaaattatga
      661 agatgagaat tgaatttttg aatgataatg gaaatgtagt aaagactatt gatagtgact
      721 ataaaacttt catagaattt attgagaata ttcattatct aacagagctc cgtaagaaca
      781 ttacttccat ttcaaaagaa ttggagagta ctctatggta tataaagttt ctatgcgatg
      841 atcttgggat tgatatgcag gatattgcaa agcataagag gattctttct atgctagagc
      901 agagaaataa aagcaatgtt gataaaaata ataaacaaaa tgagtcaact aaatttatgt
      961 tttaaaacta aatgctatga actttaatga gtatcaacag aaaattaagg taactgcaca
     1021 ataccctccc agtctaaggg taatctaccc agcactagga ttaggaggag aatctggaga
     1081 agttcttgaa aaaatcaaga aggtctacag agataaaaat ggagtattta ctaaggaaga
     1141 cattgattcc atttcaaagg aattaggaga tgtattatgg tacgtacaag ccctttgcaa
     1201 tgacctggga attagtatgc aggatgttgc agaacaaaat gtaactaaac tatcaaatag
     1261 gcttaataat aataccattc atggaaatgg tgatgatagg taaaacttaa aaaactaaat
     1321 ttatggcaat aacggttgga atatttcaac aaagtggagg tggaaaaacc atgagtatag
     1381 ttgtgggacc agatggaaca tacgatatta aaggtgaata taaaggtatg aatccaagta
     1441 ccacagtata cattaatagt gacagaaaat ttccaccatt taaactggat aattggaaag
     1501 aaggagtaaa tatgtttact acttcagatg tggaaattat aatggactta ctccaaaaaa
     1561 ttaacaatgg aactaaaatc aaatcagtta ttatagatac cattaatggt attatgatag
     1621 ataaggaaat gctggaaagt aaaaaactta cctatgataa atggatggat ttggctaagg
     1681 atatctatga aattatcaca tttgctaatt ccatgagaaa tgatttgatt atttacttca
     1741 tgggccatgt tacgttatac actgatgtag atggcaatga aagtaagtgc ttagttacta
     1801 atggaagaaa attggaaaaa ataaaagtgg aaagcaaact tcctgtagta ctattcggag
     1861 cagtagaaag aggattaaat ggtgataata agtattactt tgaaactcaa tccaacagat
     1921 ctactgcaaa aacacctcca gggatgtttg aagactttaa aattccaaat tctttggtat
     1981 atgtagacac caaaattagg gaatattatg gtatttaatt gtttaactta aatttattag
     2041 ttatggtaaa aatagtagtt gatttattca agagcatagc caagtttatt ggcttagtgt
     2101 ttatgattat ttcagtagtt ccttggaagt tgggtcagta tctattgagt attaaaacct
     2161 caaaggaaaa agaattggaa caaatactga tgggtaaaac taggacttcc aaaaaacaac
     2221 ctgtaaaaca ggaaaggaag aaaagagaaa aaagaagtaa tactaacaaa atgcatacta
     2281 caacagttga gtagtgcatt tatttgattt acttaaaaaa ttataaactt tattataaac
     2341 tttannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagtagt gcatttattt
     2461 gatttactta aaaaattata aactttatta taaactttaa aaagataaga gtatgccagc
     2521 aattaatatt aacaatagca gcaaggaagt attaaagcag tctgtagttt acacaggact
     2581 tacccaagta aaaatggttg cagttaaccc tactctaaag gaactgcaag aaatgggata
     2641 taattttcag caagagcctg tttacctaac agaggaagaa ggggttcgca agttaagatt
     2701 ggacttttat tttaagaatg caactgtatc caataagatt accttctttt tagaaaatag
     2761 acctagagtg aataaggatg gtaccaaaaa tgagtttatc aatcattatg gacaatccac
     2821 ttgggctgca gatttaaatg aagctttgaa taaaactgct tccaatggta aaaaatggtt
     2881 caatgcagaa ggagctcgtg aagcatatgt aggagaaaca agtcttattg aatttcttaa
     2941 gcagtggctt aatacaaatc ctaatgataa ggtatatatt gaggatatgg aagcactgtt
     3001 taagggaaat gttaaggaac ttcagtacta cgtaaatgct gcaaaggata atgtagtgtg
     3061 ggttcttcta acctattctg aaaaggaagg taaatattac cagaatgtgg ataatggatt
     3121 atttattagg ggatacttca aacaggtagc tgcattttct aaggctgtag agtatattaa
     3181 gcgtaaagag aaggatggat acccagtaaa gggatataat gggttggaat ttaaagaatt
     3241 tgtaccacct gctcctgaag ttattcttcc tgattcagat cttgatatgc ttaagggaaa
     3301 gattgatgat ggaagtaacg tattttagtt aacttgtaag taacggggaa aatcatagta
     3361 ttttccccgt taactttaaa ttttagtacc atgataccta tacgcaaacc tcttaccaag
     3421 gaaaatattt tgtccctaat ttcatcatat gatatattta aattttacag tcctaatttt
     3481 caaaaactca acaagttatt taaatcagac ttaagaaaag aaaataaccc ttcatgttct
     3541 attactagta taaatggaga tttactttac actgattttg gactgggaaa gagttttaga
     3601 tgtatagact ttgtaatgtt taaatataat ctaacatatg ctgaagccct tcaaaaaata
     3661 aatatggact ttaatttggg attaggggga aatattacat tggatacttc tacatctcaa
     3721 tccccaataa ttcatgaaaa aataaatatc caggataaac ctccaacaat catacaaaag
     3781 aagaaaagac catttaccaa aaaagatcta caatactgga atgagtttta ctggactgaa
     3841 gaaatgttaa aattatctaa aactgaatcc atatcacatt actggataaa tggtaatatg
     3901 ataacagttg gggatgaatt agccttttcc tatgaatact actggcataa tggaagattt
     3961 caaagaaaac tttacttccc agaaagaagc caatataaat ggataagtaa tgtggataat
     4021 actattgtac aacttgtaga tgttatgcct aaatatggtg atattctatt cataacctct
     4081 agtaaaaaag atgcaggaat attctggaga atgaatatgg agggtatgtt tagaaatatt
     4141 gtagtacatg gagtggctcc aaataatgaa gtatcttttg ttcctaagga atggtttcgt
     4201 aaaattaaaa caaggtataa aagcataatc atatggtaca acaatgattt tactggaata
     4261 aacaatgcaa gtaaattcag ttcagaatat gaaataccat attgtcataa tcctatagga
     4321 agtccaaagg atcctagtga tttttgtaag gaatatggaa ttaaaaaatt ctttaacttt
     4381 gtaacggata acttaataaa attagaatta tatgaaccct aaaattgaaa tattcatacc
     4441 aggaaatatt ccttctctta agaatagcaa aataaatgga agatttccaa gtaaaactgt
     4501 tactaaatgg cttagggaat ttggaatagt ttggtatagt tctagggata aaaaggttaa
     4561 agagtttaaa agaattccaa aaaaatattc ctttgcagaa atatgtaatc ctattaagga
     4621 atgtagggaa tatcccttac atttaggatt tttcttcatt agaaatagca gaagaaaatg
     4681 ggattttcat aatgcatgtc aaattgtttt ggatttaatg actgtatatg atataattcc
     4741 agatgataat gtagattttg tattgccttt tccattagaa atagagggaa aatactggga
     4801 cctcaataaa gaaaacccag gagtaataat aaaggtatta aattaaatgt taaacttaaa
     4861 aattaaatta ctatgagcca agtagaaaga cttagtgcta agagaaaagt cagttttaaa
     4921 ctgcctgcaa ctgttcaact gatttctatt gaatcagacg caaaaacttt tggacagtta
     4981 atggaagatg tagctaacag cgttattgct gataaaattc ctactcacag tgaaagggtg
     5041 gataataatt atgttagaac ttataagttc atagagaaga actctcttgc ggagtatgga
     5101 aaattagatg atgctgttct accagagggt aatattatac tctttgttac tcctgtagac
     5161 cataaagcag gtctaactat aaactttgaa atgaattggc cattagaacg taaaattgaa
     5221 gccattaata acatgtctta caatgaaatt aggtctcttg gctatatttt gaatcaagaa
     5281 cacaacgcag gtattagtct ttcggggagt aaacgggagg ttatcaataa tattatagag
     5341 tttctcaaaa ctagggaatc aacccctaat tttgtagagg aacttgagct attgaaaaaa
     5401 gatctccagt tcttaaagga agccctattt acaaattctg ttactgtaga tttaatgata
     5461 gataaagtaa ataacctaat cgaggcaaac tctaacaaag acagtactga agaggatctt
     5521 ggattactgg atattcataa taacattatg gaaaagttta aagaacttta atcctaatcc
     5581 tattttgtaa gtgtaagggg gaatttttgt tcccccttac tatttattaa caataatact
     5641 gaattatatg gaaagattaa aaataattaa taaactggta ttagggcata atacattgct
     5701 acttcaactt caaaaattat ctgacgaaga aagagaaaaa atatttgagt acagcaaatt
     5761 ttatagacct gaaagtttag tactaatacc agatagttat ggtagtaatt cttttatcct
     5821 tggaaaaata atttcgagaa ctcataagaa aatagtaatt ttagcagata gtggaataat
     5881 agagattttt ggtttaaata gcatagacaa aagtgtaatt gctctaaata aggaaaacta
     5941 tcacgattta tacaaattaa aactgaacca attatcgcct aaaaaactac aaaaagtagt
     6001 tgaatccata gtaaaaagta ttaacataat tcatgattat gaaattatac aaatttcatc
     6061 tacagaaatt tcagtaaatc tatatttacc tgaaatagaa ataaaaaatt ctttggagat
     6121 gagtcatatc atgagggaat gttatataaa atttactctg gtatttacaa ataataaaat
     6181 agaagtaagg aatttacaat tttatagggc caattttact ccatcagaag ttagtgcaga
     6241 ttatttattc tctcatagta atacacatcc tggagaagtt gctagggatt tttgttttgg
     6301 aggctcagaa ttgagagaat atatgagagg tgagagattt tatgtaaatg acctaaaata
     6361 tgtaatatac ctaatactac actatttcag ttgggaaagt ttggaaggag tgccctatag
     6421 aagaattgaa aatatacttc catataaaat tgaaactatt aatgattctt ctttaacaga
     6481 tgatgaggat ttcaagattt tactaaaaag tttaattgat aatccaatcg acctgagtat
     6541 taattttaat gatttctaca gtgatctttc ttctgatcta tcagttgatg aagaatttag
     6601 aaataaatta ggggaaagaa tacaagaatt atttggggaa aaatacatga cctataaact
     6661 tggggaaatg gaaggaaaat tgattagtaa aactaaagaa acagtaaaat taatttataa
     6721 ccaaactttt aagtttaaaa atcaggtaat taaacctatt atagaagaaa gtgagtatga
     6781 aaaaaagata ttggaaagtg cattagcaca atgtactaaa gtagtaagaa gagatactat
     6841 aaactatgtt tgtagtgttt tatacctaga ctatatacta caaaatttat tggcaagttc
     6901 actatttact aactaaatat ttttaaatta tgaataagat agttattagt aaactattaa
     6961 aacaacaaat tgactacttt catacaaaga taaaaaatac agagtggtca ggaatactac
     7021 tatttgatgt agtaaaagga aattttaaaa agttagagga tattgaaatc tatgctaaat
     7081 ctatgcatct catgaatatt ggaaatagta cttatacaga attcacagta gatgcagatt
     7141 tgataaaagc atataataac attaatgacc atgaactaaa atctcttaat ggatttattc
     7201 acacccatca tactatgaat gctttcttct ctggaacaga tttaaaggaa cttgaagata
     7261 atgcagataa atataatttc tttgtttctt tagtagttaa catggaagga gagtatgtat
     7321 gtaaaatagc cattcctact aaaactataa caaagaaaac cattatattc aaggatcatc
     7381 aagaaaatat tgtaaaaaga aacacagaag aagaaagtac agaaatactt tacattgatt
     7441 taaaaccagt atttgaagaa gaagtttcag taccaaaatg ggtagaagaa aactatttag
     7501 aagttaaaaa taagaaaact gaaaattttc aaaataccta ctttaaaaat aatgattact
     7561 atgtttcaaa ccatactgaa cggacttcta atagtaaaga ctatttacca agtagtaatc
     7621 ctaataaaat atataaatgg cccaatattc caaattccta tactccagat ccttataaat
     7681 ctactttata cagatctgat tcctacagta aaactgataa agaaacttgt atgaacagtt
     7741 caaatggaga gtatactaaa tgcttactgt gggtaaaggc tatactaaat gcagatgata
     7801 atgcagattt gaaagaactg gaaaaaagaa atgtaggtga tatcctaaaa gatctaataa
     7861 aatctaccag taaagatttg gaaatgtatg aaatgtatgt ggatctactg gaaccaataa
     7921 tagaacagaa ctatgaagaa atattttcag atgatccaac atttcttaaa tacccaacaa
     7981 gagctgatat tgcaattaaa ataattaaca atattcctgg gattgattta aatcattatc
     8041 tagttgaatt aataattgat actttgaaaa ttggaggaaa ttaatatggg atactacgaa
     8101 agatttaaag atgcaccttg gtatgaacaa tccgctaatg aatatattac tataataggt
     8161 gctggaggaa taggctctaa cactgcattt aatctaataa gaactttaaa atctaagata
     8221 gaaatattag attttgatat ggtagaagag cataatatag gtactcaatt ttacctaaca
     8281 aatcaaataa atagttctaa ggtaagttcc cttaaatata ctatgggatt atttgaccct
     8341 acttcttata gtagggttag atatattcaa gccaaactat cagattacaa tggagtttac
     8401 ccaataacca tatgtgcagt ggataatatg gatgccagaa aagaagcatt tgaattatgg
     8461 aaaaatttgg aaaataggga actatttata gatgggagac taacagcaaa tatctatcaa
     8521 gtttttactg ttactccaga taaggaagaa ttgtatgcta aatctctatt tccaaatagt
     8581 atgatagagg atggaccctg tacgtttaag caaactagct attttgcttc aatgattgga
     8641 gcaaggataa ctcaaatggt agttaattac ttaattaata agtatgctga aaatactata
     8701 tgtaatctac catttgcaat aatggaatct tctgaattag tattatttaa aaccgcttca
     8761 agttatgata atctgtaaga ataacgaagg aattccagta atatttagca tgtttcctga
     8821 agatactcag tatttggagt tccctaataa ttttaatttt tataggttca tgccaatgga
     8881 aattttacgc ttaaaagatt taggaaagca ttacttcgat tctctagatc catccaaagg
     8941 aataatcaga atatctacca gtaacatatt cattaatatt atagtctttg caggtatggt
     9001 aaatatagca aatgattatg gaaattctat gaatatgata atagttaaaa atagaaatgg
     9061 taaaagagta gggtatgtta aaactaaaat agtaaacaat tcaggattat tttcaaatag
     9121 tacatttcaa ctattaaaaa gaatagccag atttcttgga gttaaggaag taataataaa
     9181 taactacgag tataataagt acacaatagt ttctaaatta aacgctaata ttacaaggaa
     9241 taatggaaaa agtgaaaaat taactgcagt aaaccaagaa tatcttatta aagtctatga
     9301 tagtatggta tttgataatc tactaactaa tatagcagga gaacattcaa atatttattc
     9361 tactgataat ggtatacctg aaaatactga tatacatgtg ctatctacat tggttaaaaa
     9421 attctttaat tttaactatg atttctacat aaataccaga gataataata atattttcta
     9481 tatggtattc agaaaaaaga atagctggat aataagtaaa aatatggatc atgaagtaga
     9541 acctttatat gtaataggaa gtgttggata taagttaatg acagtaaata gagatagtaa
     9601 agtagaacta ctgggaccag aagaactaat attctttaat gcagatatgt ggaatgattg
     9661 gaatgtaaat gatcaaacca tatttatatc aaaattagat gaaacattga acaatagtga
     9721 tttttatttt tcacgttggt taaattatca taataatgaa acttttttta aaaatggaac
     9781 tttaataaaa gcactagatt tagtatctag tacgatagta aatactaatt taagttaata
     9841 aaaatctaac aatatgaaag tattagcact tatagacgct gattctttgg tttaccagtc
     9901 actaagagat tccctagagg aatctcttag tgttttagac cataaaataa acaatattgt
     9961 ggaaaaaaca aaatgtactc actatgctct atttgtttct actggaggaa attacttcag
    10021 aaagaaagtg gcagacagtg tatataagaa aaatcgtgat aaatatcaga ttagacaatg
    10081 gacaaaaaca ctaaaagcta tactggaaga caggtaccat gcaaataact gtgttggggt
    10141 agaggctgat gatttagttt tatactggta taacaagccc cttttcttta atggtaatag
    10201 tattgtggat agtgtggagc catcatccaa tgttgaggaa gttactaaaa tactatgtag
    10261 cattgataag gacttactaa aaactgttcc aggaaaacat ttcaactaca cttttaaaac
    10321 taaccctgaa aataatgaaa ctataatggg ccaatggata gaaactacag aagaggaagc
    10381 agaggaattt ttacttaaac agttagtaac aggagattct actgataaca taattggatt
    10441 accaggaaaa ggagaagtat tttggaataa gtggaaaaaa gcacaaagcg ataaaattag
    10501 tattggaaat attttagatt tgtactgtaa agaaatttat tctaaatcta aagccataaa
    10561 aactttttac aataatttta ctatgcttta catgctaaga actccagaag attttgaagc
    10621 caatggattg gagttacctt cttttccatt tatacacgct gtacccaaca atcaaatgct
    10681 tattgatgta gaattttaat catttataaa ctgtttgtat atgaaaagta aaacatttaa
    10741 tgtaaagata gatgtattaa aaaagattag gtctagggtt gagatagtgg tagaagcaga
    10801 tagtatttct gaagtctatc acagaataaa aaatctagcc tatgttagtg attttaaaaa
    10861 aaagggagta actttgctat cagaaaaaga ttctccagat tactctcttg aaatgattag
    10921 tttaagaaag gtagaggaac tacctgtaca aactaggttg gattttgatt aaattataaa
    10981 tttttttaat ttggttttta tgttgtcttt aaaagtggag ggaggaaatc aagtttatta
    11041 taggctcttg aatggtagaa tgctagagat atatacagat agtaattctg ttatggaaaa
    11101 tattgaagaa gaaactaata gaatactatc agtatctgta ggagattttg ccataaaaat
    11161 aggagattat ttccaacatg aaagggaaga aattcctagt tcttactatg ttgaggaaat
    11221 aatgagggga ggaagtaaac cccattttaa acgagcctat accattagaa gttataaaag
    11281 aaataaagga acaaattaca ttttaccctg tcttggaaat gactatctat tctttgatac
    11341 taatgattac ctaataaatt catacttaaa tatagatttt actagactgt atttactata
    11401 ccgtttttcc aaaacagagt actattctaa aattgaatcc agacttgtaa atcatccaag
    11461 atttgtaact actgattctt ctattccagg atttgatatt tttatattta atattccaga
    11521 ggaacatcaa gaagatgcca aattatttat gaagggtaag tattccaaat tatctgataa
    11581 cttaaagttt gacattaaat ccttttataa acttacaaat aacagtttta cctggaaggt
    11641 tttaaccaaa gatccaaaac taatagccca aatggaaaca aaatttggat taactaaaat
    11701 atttcattcc attgatttgg atgaaatacc tgatccaaat gaagaaattt gggaaaggtt
    11761 tataaaaaat tttaagtagt atgtatttgg aatatactat cataatacta accaagatat
    11821 tacatgttat agcattactt gtaatattta tggtatctta tgtaattaat taccttttta
    11881 ctaagttttt actaaaaatc ttctttgata ttccaataaa taataatatt cttacagaag
    11941 taaaaaattc ccttcaccaa ttagtaatat ttttaagaaa tttataaact aaactacact
    12001 acaatgaaag acgtgttaaa aaaatggttg agtaatggag ttatatatga ttcaattggt
    12061 attcaaatat atattccaga aaataatgcc tatgcaaagc atccaattcc attattaaat
    12121 atacagggag aaagttatat aaagaatttt ttaggtttaa atgatgaaga tgtattaaag
    12181 tttcaagacg aactgggaaa atttattgct gaagctatca atgaaaaaat agctcgtagt
    12241 caataattct ttgaataaca tttaataagt agaagatggg ggactttaga attaataatt
    12301 tttattattc tattagtcct ccatctttta tagataaatc aagaactatg gaacctttta
    12361 taatggacta tctagggaat aacataaatc aagatctttt tttacagata ggtagaaaaa
    12421 ttgcatctat tagaaaaaca cgagtagtct atccaaactc atctgaagta ttcagagctt
    12481 ttaaagaaac cccaatacag cacttgaaag tagtgctatt atttcaggat ccataccata
    12541 atgggagtgc tgatgggctt gctacatcct gtagcagaac aggtatctca acaacactac
    12601 aaattatgct acgggaaata gatttggagt atccagaatg gataaatgat ttaaattatg
    12661 gaagagagga tagaaaaaat ttatcaagat gggcaaaaca gggagtattg ctattaaata
    12721 ctgcgcttac agttgaaaag ggaaaacccg aaagccatac aaaaatttgg gaaccaatca
    12781 caacagcagt agtaaaaact attaattcca aacaggatat tgtatgggga ttatttggaa
    12841 ctagagccca ttactacaaa aaattcataa ctaatcctac acatactgta ttcaaagtgg
    12901 cacatccagc agcagaggcg tatggttcta aaggatttat aggatgtgga gtttttagaa
    12961 aaatcaatga agagcttgag gcaagaaata aaagtattat tcaatggtaa aaagggtaga
    13021 gggaaataaa aacccctcta ccccc
//
LOCUS       DAOSAB010000003         9758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_5205_length_9758_cov_3.675255, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000003 DAOSAB010000000
VERSION     DAOSAB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5205_length_9758_cov_3.675255"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /gene="ruvX"
                     /locus_tag="PK432_00120"
     CDS             complement(<1..299)
                     /gene="ruvX"
                     /locus_tag="PK432_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOK59370.1"
                     /translation="MNIQYPILAIDYGTKRIGLAVSDSKGLIASPLSVLNVTKNMGID
                     KIIEEIKTIIQEYRIKTILIGRPQAFQSAYTKSLKKIDSFAQKVKNDINIPVIFY"
     gene            complement(301..2088)
                     /locus_tag="PK432_00125"
     CDS             complement(301..2088)
                     /locus_tag="PK432_00125"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOK59371.1"
                     /translation="MRKLTYVQLRNEYIDFFRNKDHIQIPNAPLVPEDDPSVLFVNAG
                     MFPLVPFLKGEKHPLGNRLVNSQRCLRTGDIDEVGDEYHCTTFEMLGNWSLNDYFKKE
                     AIEMTMEFFIDVLQMDINRIYVSVFKGEDDIPKDTTSIEIWKSVFSKYGINANVGNNE
                     RIQEFGKKKNWWGLATGGPCGPDSEIFFDTGKQPCNNNCNVNCNCGKYVEIGNNVFME
                     YLFENGKYLPLGRHNVDFGGGLERIAMISQNVNSVYEVDIYKPILDEVKKLSKNNKLH
                     SQRIITDHIKAAVLVIMDGVTPSNTMQGYILRRLIRRAMRHARIVGIENGFTSTIGNI
                     CIDQFKQIYPQLDKQRENILNELKKEEEKFNKTLDKGLKELDKYISHTNTISGEEIFT
                     LYETYGLPKEVTEEILSEKNITINNPNGFEEAKRQHQEKSRSSSAGIFKGGLADTSEM
                     STKYHTTTHLLLAALRKVLGDHVYQKGSNITPERLRLDFPNQENLTQEQLQKVEDLVN
                     QAIKEKLPVTYEQLPKEEALKLVPFAAFKEKYGDTVKVYYIGDKKNPFSVEICNGPHV
                     DNTSVLGTFKIIKQESVGNGVKRIKGVLN"
     gene            complement(2112..4040)
                     /locus_tag="PK432_00130"
     CDS             complement(2112..4040)
                     /locus_tag="PK432_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59372.1"
                     /translation="MSSRIKPTKSKNKELFLTLLLLLTLPVFVFSIIENRSFDTRNKA
                     FEEIMLSKLNPCIITFPNVNPYTLEVGKTVRIQVDAISDSSSIKEITITDERGETLFN
                     KSYDSTINTRVTESFAYTPKFEGAYNLTGTLIDMTGKSFACVISSPYKAADEKAVKAI
                     TSNSKPEFLTTPQASKPSQSIKVGETYEYTLEAQDTDKDTINYAFSFTKGETWLKATV
                     INDGGNGQLSIKFRGTPKRAGSYLANVFIHDGYSMHLSSQSWVISVSPEGNDNPVVTI
                     IDPVSDMRITQDTKLTVTWEAIDSNQILRYEIYITSNPANESAWKAIDTKIPPTQNSY
                     TIDLSGLENGAYRVIVRAIDDQDPAGVGIDVSKEIIIAKEEEEGPDDQPQLPQPQIIN
                     FSPTSTDEISNTSPTIKASLIATQNNTIEEDSIVFKIDDIDKTKELKINKISDSEYTL
                     IYIPETPLADGLHKVSVSFKDSSGEDITKEWTFTISGTQQTDSFNIFGLEIPKRTIYI
                     ILGGIALILLAIFIPSVISSLWKEDSKQASAENMVLPESIPPTPPSEMPQPSPQPTES
                     TAQPQKPKEVFQAPEPEIPQMPQPSPQPTESTAQPQEPQATFTAPEPQEDLSSLYEEI
                     KKLEEETQTKESTDNSTE"
     gene            complement(4058..5368)
                     /locus_tag="PK432_00135"
     CDS             complement(4058..5368)
                     /locus_tag="PK432_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK59373.1"
                     /translation="MDEKRKKLLKILAIVVGFVIVLSILMVILSNSGKPKQVELTFWG
                     FWEEEEVMHPLIEKYEAENPGVKITYAIQPLKNYESLLYTRLAQAEISNEPAPDIAMI
                     HNSWTPKFRKYLSPLPSSVMSTDEYSKEFYPTAIEDFTGIDGEIYAIPLQIDGLMIIY
                     NKELLKEAGYNAPPTDWDSFMKAAEDLTKRDSSGKIIQSGLAIGTSRNITHSTDILFY
                     FYLQNLAQILSEDKTMMNLTSPRALAAFDKYTSFVREKNPTWASYLPNDLRYFVDGKV
                     AMMFGTSWRALEILEYTEDIEFGLAPLPRLTNNDEVYYATYWGTSVSKTSKDTLEAWK
                     FVKFLSEPEQLRRLYQNAAKTRAFGEPYSRVSMNAELAENKYTQALAYMAPYMKSFQV
                     GEQAFVEEKLRQAITAVAENRRSSNSTLQTIQNEINARLAVSNK"
     gene            complement(5387..6181)
                     /locus_tag="PK432_00140"
     CDS             complement(5387..6181)
                     /locus_tag="PK432_00140"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HOK59374.1"
                     /translation="MDEDIQIKKVSEKKISETKKTISWILAVIGIVIILSQAIPLLVS
                     YAQGQIQLSKQNSMKDPLPESYKQYIQNEFAYYDPGKSYFENLSKTAEGLQYQGLFSY
                     DPTTKTTKEIVVNKEYKKNMYLSISEAGIEKVRITPNVESSEEKVYDQYLKYGLAHFK
                     GTPLPGDGGNSFIYGHSAVESFFKSHSNLPETIFTRLENVDIGDTVEIYRDDQVLKYI
                     VRNKKIVSADDLSILQTQSDKETVTLMTCWPLGAGIKRLVVVAERI"
     gene            6264..7361
                     /gene="dnaJ"
                     /locus_tag="PK432_00145"
     CDS             6264..7361
                     /gene="dnaJ"
                     /locus_tag="PK432_00145"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOK59375.1"
                     /translation="MTKRDYYEVLGVSKDAKKEDIKKAYRRLVKTYHPDVNKEADAEE
                     KFKEIQEAYEVLSDDAKRQAYDQYGHAGTQGFDPRSGNGGGFEYTYGGAPFDMGDIFN
                     TFFGGSDFGFDFGFGEQTTRRQRVSKGEDIRYRMRFSFEEAMKGGEFSVRIKRKVKCS
                     ECNGTGSSTGKRKTCDVCKGTGRERRLQNSFLGQISVMTECSKCGGLGTIPESVCSKC
                     GGTGLESKTEDMKVKIPAGAYDGMVLRFREGGNASLDGEYGDLYIELEVEPSEKFDRR
                     GNDLYSVEYIPVHTAVLGGTVEVETIDGKVKLKIPAGTQPGTIFKIKGKGAPILRKEN
                     TRGDLYVKVNVDIPKRLKKEERRLWEELSNI"
     gene            7481..8602
                     /gene="nusA"
                     /locus_tag="PK432_00150"
     CDS             7481..8602
                     /gene="nusA"
                     /locus_tag="PK432_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOK59376.1"
                     /translation="MAMTSELVAAINQISAERGIDKEEVFKALESAILSAVKKEKYGR
                     VENEDNEEEKAGSNLVVEVNRETGEFKLFALKEVVKRVKNPEKEIALSEAELISPNVE
                     VGDSIQIEMPSEDLGRIATQTAKQVLLQKIRESEKESVLSEFSDKVGQVFTALMQRMQ
                     KGTAVMEIGKAVAYMPPEEQITNEFYRVGERYKVLLKSIEDSDMIVSRSDPNFLIELF
                     KLEVPEIESGIVEIKAVAREAGSRSKIAVVSHQEGIDPIGSCVGQRGMRIANVMNELG
                     EEKIDIIEWDEDLEKFVANALSPAKVESVNISGDTATVVSPSDQLSLAIGRDGQNVRL
                     ACKLTGVKIDIVGAQGEEVDAVEEYAEDTQEEVVDKKEE"
     gene            8633..>9758
                     /locus_tag="PK432_00155"
     CDS             8633..>9758
                     /locus_tag="PK432_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOK59377.1"
                     /translation="MVKGAKKDKTSVQGRIPIVTILGHVDHGKTTILDYIRKTNVQSC
                     EAGGITQKISVFTVAIDEDSSKRITFIDTPGHEAFDLMRVRGGSIADIVLLVVAANDG
                     VKPQTVESIDIIKNSSAKPIVVINKVDLPDVDIAKVKRDIVNEGLLLEGMGGNIPVIE
                     VSGKTGKGIPELLDLITLVAEVEGLQDRGELPNGVVAKAFVLESVRDKTRGNISSIVL
                     VNGNLCKGSWLGYSKDGKVVVERIKGVISEDGNNICDLQCGCGGKIIGISNLIPVGSE
                     VYILEENNPKLIEPLLKSKNIESETAITEDDIFNSIFDDQQDNEDTQLNVVIKCSSQG
                     SLEALRHSLEKIKKDEYKINIVSDGVGDISNKDIEMAKLSK"
BASE COUNT         2913 a         1484 c         1877 g         3484 t
ORIGIN      
        1 tcataaaata ttacaggtat gtttatatca ttttttacct tttgtgcaaa agagtctatt
       61 ttctttaaag acttagtata tgcactttga aatgcctgag gtcttcctat tagaattgtc
      121 tttattctat actcttgaat tattgttttt atttcttcta ttatcttatc aatccccata
      181 tttttagtta cattaagtac acttaatggt gaggcaatta agcccttact atctgatact
      241 gctagaccta tcctttttgt cccgtaatct attgctaata tagggtattg tatattcata
      301 ttagtttaga actcctttta ttctttttac accatttccc acactttcct gttttattat
      361 cttgaaggta cccagtacag atgtgttatc aacatgtgga ccattgcata tttctacaga
      421 aaacggattc tttttatcac ctatgtaata tacttttaca gtatctccat atttttcctt
      481 aaatgctgca aaaggaacaa gctttaatgc ttcttctttt ggcagttgct catatgtaac
      541 aggaagcttt tcttttatag cttggttaac taaatcttct actttctgaa gctgttcttg
      601 tgtaagattt tcctggttag ggaagtctag tcttaatctc tctggtgtaa tattacttcc
      661 tttttgatat acatgatctc ctaatacttt tcttagtgcc gctaataaaa ggtgtgtagt
      721 cgtatgatat tttgttgaca tttctgatgt atctgcaaga ccacctttaa atattcctgc
      781 agaagagctc ctcgattttt cttggtgttg tctttttgct tcttcaaatc cattagggtt
      841 gttaatagtt atattttttt cagaaagtat ttcctctgtt acttccttag gcaagccgta
      901 ggtttcataa agtgtaaata tttcttctcc tgatattgta tttgtatgag agatatattt
      961 atcaagttct tttaatccct tatcaagtgt tttattaaac ttttcttctt ctttttttaa
     1021 ttcgtttaag atattttctc tttgtttatc aagctgaggg tatatttgtt taaactgatc
     1081 tatacatata tttccaattg ttgaagtaaa accattctca atgccaacta ttcttgcatg
     1141 tctcattgct cttcttatta atcttcttaa gatatatccc tgcatagtat ttgatggtgt
     1201 tacaccatcc attattacaa gtactgctgc ttttatatga tctgttatta tcctttgtga
     1261 gtgcagttta ttgtttttag agagcttttt aacctcatca agtataggtt tatatatatc
     1321 aacctcatat acggaattta cattctgact tatcattgca attctttcaa gacctccgcc
     1381 aaaatctaca ttatgtcttc caagaggaag atactttcca ttttcaaata ggtattccat
     1441 aaacacattg tttcctatct ctacatattt cccgcaatta cagttaacat tacagttgtt
     1501 attacatggt tgttttcctg tatcaaaaaa gatttctgaa tcaggcccac atggtcctcc
     1561 tgttgctagt ccccaccaat tctttttctt tccaaactct tgaattcttt cattatttcc
     1621 aacattggca ttgataccat attttgaaaa tacacttttc catatttcta tagaggtggt
     1681 atcttttggt atatcatctt ctcccttaaa cactgaaaca tatattctat ttatatccat
     1741 ttgtaaaaca tcaatgaaga attccattgt catctctatt gcttcttttt tgaaatagtc
     1801 atttaaagac caatttccta acatctcaaa tgtagtacag tggtactcat ctccaacctc
     1861 atctatatca ccagttctta agcatctttg agaatttaca agtctatttc ctaatggatg
     1921 tttttctcct tttaaaaacg gaacaagggg gaacattcca gcatttacaa aaagtactga
     1981 gggatcatct tctggaacaa gaggagcatt aggtatttga atatgatctt tattcctgaa
     2041 aaaatctata tattcatttc gcagttgtac atatgtaagt tttctcattt gataattata
     2101 tcagaaagga tttactctgt agagttatca gtactttctt ttgtttgagt ttcttcttct
     2161 aatttcttta tctcttcata gagtgatgag aggtcctctt gtggttcagg ggctgtaaat
     2221 gtagcttggg gttcctgtgg ttgggcagta ctttctgtcg gttggggact aggctgaggc
     2281 atctgtggta tctcaggctc aggtgcttga aatacttctt taggtttctg tggctgggca
     2341 gtactttctg tcggttgggg actaggttga ggcatctcac ttggaggggt aggcggtata
     2401 gattctggaa gtaccatgtt ttctgctgat gcttgtttag aatcttcttt ccataaagag
     2461 gaaataactg atggtataaa tattgcaaga agtattagtg ctattcctcc taatattatg
     2521 tatatagttc ttttaggtat ttcaagacca aatatattaa aactgtcagt ttgttgtgtc
     2581 cctgatatcg taaatgtcca ctctttagtt atatcttctc cactagaatc tttaaatgat
     2641 actgagactt tatgtaaacc atcagcaaga ggtgtttcag gaatatatat taatgtatat
     2701 tcagagtcgg atattttgtt tatctttaat tcttttgtct tatcaatatc atctatctta
     2761 aatacaatgc tatcctcctc tattgtatta ttttgagttg cgataagaga ggcctttata
     2821 gttggtgagg tattgcttat ctcatcagta cttgtaggag agaaattaat aatttgtggc
     2881 tgaggaagtt gaggctgatc atctggaccc tcttcttcct cttttgcaat aattatctct
     2941 tttgaaacat caattcctac tcctgctgga tcttggtcat ctattgctct tactatcact
     3001 ctgtaagcac cattttctaa tcctgataga tcaatagtat atgaattttg agttggtggt
     3061 atcttggtat ctattgcttt ccatgctgat tcgtttgcag ggttagaagt aatatatatt
     3121 tcatacctta atatttgatt gctatcaatt gcttcccaag ttactgtaag tttagtatct
     3181 tgtgtaatcc tcatatctga aactggatct attattgtaa ctacagggtt atcatttcct
     3241 tctgggctta cacttataac ccatgattgt gaagaaagat gcatgctata tccatcatgt
     3301 ataaagacat ttgccagata gcttcctgct ctcttaggtg ttcctctaaa ctttatactt
     3361 agttgtccat tcccaccatc attaattact gtagccttta accatgtttc tccttttgtg
     3421 aatgagaaag cataatttat tgtgtcttta tctgtatctt gtgcttcaag tgtatattca
     3481 tatgtttctc ctactttaat actttgtgat ggtttagatg cttgcggggt agttaaaaac
     3541 tctggtttag agttagatgt aattgctttt actgcctttt catctgctgc tttgtatgga
     3601 ctgcttatta cacatgcaaa actttttcct gtcatatcaa ttaatgtacc agtgaggttg
     3661 tatgcccctt caaatttagg tgtatatgca aaagattctg ttactctagt attaattgta
     3721 gaatcgtaac ttttgttaaa tagtgtttct cctctttcat ctgttattgt tatttcctta
     3781 atagaagagc tatcggatat agcatctacc tggattctta cagttttacc tacttcaaga
     3841 gtatatggat taacatttgg gaaggtaata atacagggat taagcttact gagcatgatt
     3901 tcctcaaagg ccttatttct tgtatcaaaa cttctgtttt ctataatact aaatacaaaa
     3961 acaggaagtg taagcaatag caggagtgta aggaacagct ccttgttttt actttttgtt
     4021 ggcttaattc tgctactcat ctaatatata ttattcttta tttattgctt actgcaagtc
     4081 tagcattaat ttcattttga atagtttgta gggtagagtt actgcttctt ctattttctg
     4141 ctacagcagt tattgcttgt ctaagttttt cttcaacaaa tgcctgctct cctacttgga
     4201 atgatttcat atatggagcc atgtatgcga gagcctgtgt atatttgttt tcagctaact
     4261 cagcattcat agatactctt gaatatggtt caccaaatgc tcttgtttta gcagcatttt
     4321 gatagagtct tcgtaattgt tcaggttcac ttaagaactt tacaaatttc catgcttcta
     4381 aagtatcttt acttgttttg cttacgctag ttccccaata tgtagcatag tagacctcat
     4441 cattgtttgt aagacgtggt agtggagcaa gcccaaactc tatatcctct gtgtattcta
     4501 aaatctcaag tgctctccag ctagtcccaa acatcattgc aacctttcca tcaacgaaat
     4561 atctgagatc gttaggcaga tatgatgccc aggtagggtt tttctctctt acaaatgagg
     4621 tgtatttatc aaaagcagca agtgctcttg gagatgtaag attcatcatt gttttatctt
     4681 cactaagtat ttgggctagg ttttgaagat agaagtagaa aagtatatct gttgagtgag
     4741 taatatttct tgatgtgcca attgctaagc cagattgtat aattttcccg cttgaatctc
     4801 tttttgtaag atcctctgct gctttcataa atgaatccca gtcggttgga ggtgcattat
     4861 acccagcctc tttaagaagt tctttgttgt atataatcat aaggccatca atttgaagag
     4921 gaattgcata tatttctcca tcaatcccag taaaatcttc tattgctgtc ggataaaatt
     4981 ccttactata ttcgtctgta ctcattacag atgaaggaag aggtgataga tattttctga
     5041 attttggtgt ccagctgtta tgtatcattg ctatatcagg tgcaggctca tttgatattt
     5101 ctgcttgtgc aagtcttgta tacagtaaag attcataatt ctttaatggc tgaattgcat
     5161 atgttatctt tacaccagga ttttcagcct cgtatttttc aattagaggg tgcattacct
     5221 cttcttcttc ccagaagccc cagaaagtta gttccacttg ttttggttta ccactattac
     5281 ttagtattac cattaaaata gagagtacta ttacaaagcc aactactatt gcaagaatct
     5341 ttaataattt ttttcttttt tcgtccatac atatatatta cacaaattaa atcctttcag
     5401 ccactactac cagtcttttt ataccagctc ctagtggcca gcatgtcatt agtgttacag
     5461 tttctttgtc tgattgtgtt tggagtattg aaaggtcatc agcagaaaca atctttttgt
     5521 ttcttacaat atattttaaa acttgatcat ctctgtaaat ctctactgta tctccaatat
     5581 ctacattctc taatcttgtg aatattgtct cagggagatt agaatgagat ttaaagaagc
     5641 tttcaacagc actatgcccg tatatgaagg agtttcctcc atcaccagga agtggtgtac
     5701 ccttaaagtg ggctaatcca tattttagat actggtcata aaccttttct tctgagcttt
     5761 ctacatttgg agttatcctc accttctcta ttccagcctc agaaattgaa aggtacatat
     5821 tctttttgta ttctttgttt acaacgattt cctttgttgt ttttgttgtt gggtcatatg
     5881 aaaaaagtcc ttgatactga agtccctctg ctgttttaga taaattctca aagtagcttt
     5941 ttcctggatc ataatatgcg aattcatttt gtatatactg tttgtaagat tcaggaagcg
     6001 gatctttcat tgagttttgt tttgagagct gtatttgccc ttgtgcatat gatactaaaa
     6061 gaggaatagc ttgacttaat ataattacta taccaattac tgctaatatc caagagatag
     6121 ttttttttgt ttctgatatc tttttctcac taactttttt aatttgaata tcttcatcca
     6181 taagtaggaa tatattatca attaagtgtt tcaatgtcac atatttggta attaattcat
     6241 ttgtctgcta gaatattgtc cgtatgacaa agagagatta ctatgaagtc cttggtgtaa
     6301 gcaaggatgc aaaaaaagaa gatataaaga aggcatatag aaggcttgtg aagacgtatc
     6361 atcctgatgt aaataaagag gctgatgctg aagagaagtt taaagagata caagaggcat
     6421 atgaggttct ctcagatgat gcgaagagac aagcctatga tcaatatggt catgctggta
     6481 cacaaggttt tgatcctaga agtggaaatg gaggtggctt tgagtatacc tatggaggtg
     6541 ctccctttga tatgggggat atatttaata cattttttgg tggaagtgat ttcggatttg
     6601 attttggatt tggggagcag acaacaagaa ggcagagagt ctctaagggt gaagatatta
     6661 ggtatagaat gagattttca tttgaagagg caatgaaggg aggagagttt agcgtaagga
     6721 taaagaggaa agttaaatgc tctgagtgta atggaacagg aagttctact ggtaaaagaa
     6781 agacatgtga tgtctgtaaa ggtactggaa gagagagaag acttcagaac agctttttag
     6841 ggcagatatc tgttatgaca gagtgttcaa agtgcggagg tctgggaact attccagagt
     6901 ctgtttgtag taagtgtggt ggtacaggcc tcgaatcaaa aacagaggat atgaaagtta
     6961 aaattccagc aggagcatat gatggtatgg tattaaggtt tagagaaggt ggaaatgcct
     7021 cattagatgg agaatatggt gatttatata ttgaattaga agttgagccc tcagagaagt
     7081 ttgataggcg tggcaatgat ttgtactctg ttgaatatat tcctgtgcat acagcagtac
     7141 tgggtggtac tgttgaagtg gaaacaatag atggaaaggt taaattaaaa atacctgctg
     7201 gtactcaacc aggaacaatt tttaagatta aggggaaggg tgctccaatt cttagaaagg
     7261 agaatacaag aggagatttg tatgtaaaag ttaatgtaga tattccaaag aggcttaaaa
     7321 aggaagaaag gagattgtgg gaagagttaa gtaatatcta aatatatttg ggattttcct
     7381 gtagatgtga tatactcata ctgtattagg gttattggaa tggtgaggta tttctcacct
     7441 tttttttagt atgaataatt aaatttttaa gaatatacat atggcaatga catcagagct
     7501 tgttgcagca ataaatcaga tatctgcaga aagaggaatt gataaagagg aagtatttaa
     7561 ggctttagaa tctgcaatac ttagtgctgt taaaaaggaa aaatatggga gagtagagaa
     7621 tgaagataat gaggaagaaa aagcaggaag taacttagtt gtagaggtaa acagagaaac
     7681 aggtgagttt aaactttttg cacttaagga agttgtgaaa agagttaaga accctgaaaa
     7741 agaaattgca ctaagtgagg cagagttaat atcacctaat gtagaggtgg gtgattctat
     7801 tcagatagag atgccgtcgg aggatttagg aagaattgct acacagactg ctaaacaggt
     7861 gcttcttcaa aagataaggg aatctgaaaa agagtctgtt ttaagtgagt ttagtgataa
     7921 agtgggacag gtatttactg cattaatgca gaggatgcag aaaggtactg ctgtcatgga
     7981 gataggaaaa gcagtagcat atatgcctcc tgaagagcag attacaaatg aattttatag
     8041 agttggggag agatataaag tgctccttaa aagtattgaa gattcagaca tgatagtttc
     8101 aaggtctgac cctaatttct taatagagct ttttaaactt gaggttccag agattgaatc
     8161 tgggattgtt gaaattaagg ctgtagcaag agaggcaggt tcgagaagta agattgcagt
     8221 tgtatctcac caagaaggta ttgatcctat tggttcttgt gttggtcaaa gaggaatgcg
     8281 tattgcaaat gttatgaacg agctaggtga agagaaaatt gatataattg aatgggacga
     8341 ggatttagag aaatttgtag ctaatgcatt aagtcctgca aaggttgagt ctgttaacat
     8401 atctggtgat acagcaactg ttgtttctcc atctgatcag ttatctttag caattggaag
     8461 agatggtcaa aatgtaagac ttgcatgtaa actcactggg gtaaagatag atatagttgg
     8521 tgcacaggga gaagaggtag atgctgtaga agagtatgca gaagatactc aagaagaagt
     8581 tgtagataaa aaagaagagt aagaatttta aaatgaaaaa ctgtagttta aaatggttaa
     8641 aggagcgaag aaagataaaa caagtgttca gggcagaatc ccaatcgtta caattttggg
     8701 tcatgttgac catggaaaaa ctacaatact tgattatata aggaagacga atgtacaaag
     8761 ctgtgaagca ggtggtataa ctcaaaagat ttctgtattt actgtcgcaa tagatgaaga
     8821 ttcttctaaa agaattactt ttatagacac tcctggtcat gaagcatttg atttaatgag
     8881 ggtaagaggt ggaagtattg cagatattgt tcttttagtt gtagctgcaa atgatggagt
     8941 aaaacctcag acagttgagt ctatagatat aattaaaaac tcatctgcaa aaccaattgt
     9001 tgttatcaac aaggtggatt taccagatgt tgatatagca aaggtaaaaa gagatattgt
     9061 taatgaaggg cttttacttg aaggtatggg tgggaatata cctgttatag aggtttcagg
     9121 aaagactgga aaaggaatac ctgagctttt agatttaata actcttgtag ctgaagttga
     9181 aggtctgcag gatagaggag aactacctaa tggtgttgtg gcaaaagcat ttgtactgga
     9241 atctgtaagg gataagacaa gagggaatat atcctctatc gtgttagtaa acggtaattt
     9301 gtgtaagggt tcttggcttg gatattctaa agatggaaaa gttgttgttg aaagaataaa
     9361 aggagttatt tcagaggacg gaaataatat ttgcgattta cagtgtggat gtggagggaa
     9421 aattattggt atttctaatt taatacccgt aggctctgag gtatatattc ttgaagagaa
     9481 taatcctaag ttaattgaac cattacttaa atctaaaaat attgaaagtg aaactgctat
     9541 tacagaagat gatatattca actcaatttt tgatgatcaa caagataatg aagatacaca
     9601 attaaatgtt gtaattaagt gttcttcaca agggtctctt gaagctctta ggcattcatt
     9661 agaaaagata aagaaagatg aatacaaaat taatattgta tctgatggcg tgggggatat
     9721 atcaaacaaa gatattgaaa tggcaaaact ctctaaag
//
LOCUS       DAOSAB010000004         9081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_5638_length_9081_cov_3.240084, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000004 DAOSAB010000000
VERSION     DAOSAB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5638_length_9081_cov_3.240084"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..573
                     /locus_tag="PK432_00160"
     CDS             58..573
                     /locus_tag="PK432_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59378.1"
                     /translation="MDNEIENLEKKEVTTEDIISGIKSISPIEWKSANYPVISYANRE
                     FLDIKDKEAEKSLRFWKEVLNPEHFLNMAKSVDFDPSQNFLFIIHTFKDSKGEEVIIK
                     AITTTPESFVEDPRNMINYYRHGFPCENRGGGRYPTLNNFWVDFYDKLSSVDMDKYPI
                     LTRDWKKSNII"
     gene            653..1495
                     /locus_tag="PK432_00165"
     CDS             653..1495
                     /locus_tag="PK432_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N(6)-methyltransferase family
                     protein"
                     /protein_id="HOK59379.1"
                     /translation="MIDIKYSQNLYTNKEKLKEIIKLSEINFSDIVLDIGAGTGIITE
                     ELSRYAKQVIAYELDQKYFKKLEEKFKNNSNVVLKNENFLNVQLPKKTFKIFSNIPFF
                     VTADIINKITDVNSKLEEAFLFVQKESAERFAGEPKNTQIATILSFMYEIKIREKFQK
                     KDFKPMPNVDIVLLNIRRKDFNKKEFYLYRDFITYIFNQMNYSVLDTFKELFTYKQLK
                     YINQYLNKNRYSKPTDIPLDYYLNIFQEFKLNGKNYINKVNGYYSKHLKQHSNREKVH
                     RTRI"
     gene            complement(1527..2651)
                     /locus_tag="PK432_00170"
     CDS             complement(1527..2651)
                     /locus_tag="PK432_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK59380.1"
                     /translation="MEQTEIEQTPEREDIEYLVNKIEATHFNPYLNVSREDFINSLNK
                     AQKENEEFFPLAIQESIALTRDAHTYVKGILNNDYLPIECKEFEGHFYIIGSSDNYNH
                     IIGHEILKINEHPLSEIFPQISRLSSKENREVLLSDLSRYLPSNKILRYYNFSNSDSV
                     KLTTNNEEIDIKADSEQKIKIKEPLKWKMSEKNDPTYVGNNKYHFRLINNNLLFQYNE
                     CTNEGHTKEELTTFKNKLLNSAQNADNIIVDLRKNRGGNTKVMEDLFEKLPNNKKIYV
                     AIGRKTFSSAMHHLLFLKQEKGAILIGENAGQKPNRFGDYKEIVLPNSKLRIACSFKY
                     FELIPNSNIDVIEPDIKIPITIDDYMNETDPLNKWIVENL"
     gene            complement(2687..3163)
                     /locus_tag="PK432_00175"
     CDS             complement(2687..3163)
                     /locus_tag="PK432_00175"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK59381.1"
                     /translation="MITLISPSTNTKIIEKIVEIHTKCIKETNSQYYNNKQIDEWISL
                     INRENVKNQLKNTTWILLRENNEIIGFAQYSKKDKSIYQIQITPSKQRKGYGRQIYEY
                     IENDFRRNNINEIQLMSTLNALPFYNSLGFNITKKKNFKLKSVKIKMSEMKKVLLT"
     gene            complement(3166..3465)
                     /locus_tag="PK432_00180"
     CDS             complement(3166..3465)
                     /locus_tag="PK432_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59382.1"
                     /translation="MENREVIIENNINNNPLIEYVNDLANATNKDEIRAITDKILNIL
                     PENVSSLSIPFFDNTNGITLITSERTNNNIPEEKHEYWGIERLINYLLEEESNNK"
     gene            3550..4188
                     /locus_tag="PK432_00185"
     CDS             3550..4188
                     /locus_tag="PK432_00185"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOK59383.1"
                     /translation="MLQVVQLHKEYDRLQLKYGDPTLSSIYGAGCINNPEVMFIFMNP
                     TGKNISSSHSWKGLRAPWIGTKQVWDIFENLGLLQSKIYLKIKNIRPEEWDYDFANEV
                     YTDIQNHSTFITNLAKCTQIDARPLNNSVFKEYLDLMYKEIEIINPKKIVTFGNQVSS
                     ILLDKNISVSKYLGDEGEVLNGYEIYPTYYPVGQGRRNMPLAIGRLMNVLNK"
     gene            complement(4234..4590)
                     /locus_tag="PK432_00190"
     CDS             complement(4234..4590)
                     /locus_tag="PK432_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59384.1"
                     /translation="MNHEMKLNAKAFADVKAGLQKYETRLFDEKRSKISKGDTITFSK
                     LPELDQKVVVKVLRIIRAKDFKELFLLFPIKDANWPSDYSNYDCARNMLKYYSYKDQK
                     KFGAGAFEIELVQEND"
     gene            complement(4625..4701)
                     /locus_tag="PK432_00195"
     tRNA            complement(4625..4701)
                     /locus_tag="PK432_00195"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4665..4667),aa:Val,seq:gac)
     gene            complement(4692..4985)
                     /locus_tag="PK432_00200"
     CDS             complement(4692..4985)
                     /locus_tag="PK432_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59385.1"
                     /translation="MERDIEREENPLLKYVREIDKLSKTTEEEKLKKVIEETLNHLPQ
                     NVRSLSIPVGDSVSVTAQRECADIPEEEQRDWSLGDLIAYSVSESGLTNETGD"
     gene            complement(5013..5597)
                     /locus_tag="PK432_00205"
     CDS             complement(5013..5597)
                     /locus_tag="PK432_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOK59386.1"
                     /translation="MAFLIPTVIEKDREGERAYDIYSRLLKDRIVFLGGPIETHLANT
                     IVAQLLFLEKENPDEDIQMFINSPGGEINAGMAILDTMNYIKCDVSTIAVGLAASMGS
                     LLLAFGAKGKRFALPNSKIHIHQPLGGVQGQASDIAIEAEEILKTRDQLYRMLSKQTG
                     QSIKQIQKDADRDKYFTAQEAKEYGLIDKVISKR"
     gene            complement(5617..6660)
                     /locus_tag="PK432_00210"
     CDS             complement(5617..6660)
                     /locus_tag="PK432_00210"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOK59387.1"
                     /translation="MDTKYTRKDINEYTHELNITIPADSFKHSYDLMLQDYSKDLDVK
                     GFRKGKVPADFISNEAKDVLRLETFQRLAPLYINNVLTKENMDPIAPPEFKELPKFME
                     NLDIPFTITVTTMPTFKLGDMKKVKVPKEKLTVEQSEIDSALNELKKSQKTKTKEIND
                     EWAKEIGEIIGEKEIKSLDALKEKIKDALLKEKEHYQIHQMQDSALKLAIKECNIEIP
                     QQAIDYEAREREKAFVEDMKSRGINIDDFLKANNITIEKMRELWKLDAKEAIEADVFL
                     TLYADSRNIQMTDEELDARIEEIKKQRADVDKSIFTNPQWRENMKRVGRKEKAFNMFI
                     EEVLGKEYIDEHN"
     gene            complement(6697..7344)
                     /locus_tag="PK432_00215"
     CDS             complement(6697..7344)
                     /locus_tag="PK432_00215"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HOK59388.1"
                     /translation="MILTPLGYFLIYPNYNKLIGPIKRFNVQRSAEEITFQIITELVP
                     TRDEVLGVAIQSSGGQSIEESLNPQTVIDRKILEELNTKIIINSIDVEGLVYEGTDSH
                     TMNKGFWHFPSSNLPGQKGNMVVIGHRYAKLPPNKDTFFNLDKVKVGDKIEVVQNDNQ
                     FTYIVTETKVVEKNDISVLQDYSDYRITLITCSPLWTSKQRLVIVGKLDKLYKNT"
     gene            7485..8408
                     /locus_tag="PK432_00220"
     CDS             7485..8408
                     /locus_tag="PK432_00220"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOK59389.1"
                     /translation="MAKKKNIKKSASKVSKKEESMKNKVEIKQPSSVKQEKLSRLVGS
                     IFIGLGVLLVAFGIFSYIKYREEPLLDPELESPQIENVTSVTNGDVIKIQGSAKGYDS
                     VFIYANDEKIAEAKVEDDSKFSYEYPVEKEGEYKIAVAGVKGFPSRKISPKSEIATSI
                     VDRTAPSKEGVTLKYGTETNKNTFILVGTVEPNATVEVKRGTDSFSALADKDGNFRIE
                     GIKLEEGKNVYSVYIKDLAGNEVQLDEKVRVNYSPLGSVNGDAVVDQNIPQAAGTFDE
                     LIGNNLMMIFGLLALLAFVISSSVVYLKNKR"
     gene            8473..>9081
                     /locus_tag="PK432_00225"
     CDS             8473..>9081
                     /locus_tag="PK432_00225"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOK59390.1"
                     /translation="MKRIILLILLFITFFLVSPVYAQKEVFYHGVINGYKEIPCFKAL
                     EGSYKCFEYYVDIEELNDQKVISPILSENEESRFRKGDKVFVSFLSDEYGNEEWSITG
                     YDRNGSILLIVCCFSALALIIGGKQGFGSLVSLIFTVLILYLWAIPRILNGADVISTG
                     IISVIVMIVVIMYSSYGINIKSHIGVISCIIGVLIVGLLAKLF"
BASE COUNT         2876 a         1275 c         1393 g         3537 t
ORIGIN      
        1 cttgatatac tatattgtgg ttaagaatta agaatattaa gttttagaat ttgaataatg
       61 gataacgaaa tagagaatct tgagaaaaaa gaggttacta cagaagatat tataagtggg
      121 ataaaatcaa tatctccgat agaatggaaa agtgctaatt atcctgttat ttcatatgct
      181 aatagggaat ttttggatat taaggataaa gaagcagaaa aaagtttaag attttggaaa
      241 gaagttctta atcctgagca ctttttgaat atggcaaaat cagtagactt cgacccaagc
      301 caaaacttct tatttattat tcatacattc aaggattcaa aaggtgagga agtaattatt
      361 aaggctatta caactacacc tgaatcattt gtggaggatc ctaggaatat gattaattat
      421 tacagacatg gatttccttg tgagaataga ggtggtggta ggtatcctac tttaaataat
      481 ttctgggtag atttttatga taagttatct tccgttgata tggataaata ccctattctt
      541 actagagatt ggaaaaaaag taatattatt taattctttt attttactta atctcttcta
      601 ataatcagat actttttcaa cagtttctgt ttaatataac aaaatatagg ggatgataga
      661 cataaagtat tctcaaaact tatatacaaa taaagaaaag ctgaaagaga ttattaaact
      721 ttctgagatt aattttagtg atattgtttt agatataggt gctggtactg gaattattac
      781 agaagaatta tcaaggtatg ctaagcaggt tattgcttat gaattggatc agaaatattt
      841 taaaaagtta gaagaaaaat ttaagaataa ttctaatgta gtacttaaaa atgagaactt
      901 cctgaatgta caattaccga agaagacatt taagatattt tctaatattc ctttcttcgt
      961 tactgcagat attattaata aaattacaga tgtaaattct aagttagaag aagcattcct
     1021 atttgtgcag aaagaatctg cagaaagatt tgctggggaa ccaaagaata cacaaatagc
     1081 tacgatatta tcatttatgt atgaaattaa gattagagaa aagtttcaga agaaggattt
     1141 taagcctatg cctaatgtag atatagtctt gcttaatata aggcgtaaag attttaataa
     1201 aaaagaattt tatttatata gagattttat tacatatatt tttaatcaga tgaattattc
     1261 tgtgttagat acatttaaag agctttttac atataaacag ttaaagtata ttaaccaata
     1321 tcttaacaag aatagatata gtaagcctac agatattcct ttagattatt atcttaatat
     1381 ctttcaggaa tttaagctca atggaaagaa ctatattaat aaagttaatg gatattattc
     1441 aaaacattta aagcaacatt ctaatagaga gaaagtacat agaacgagga tataatctta
     1501 gaaacaagtt tttaagtaaa taatatttaa agattttcta ctatccattt atttaatgga
     1561 tctgtttcat tcatatagtc atcaatagta ataggtattt ttatatctgg ttctatcaca
     1621 tcaatattac tattgggaat taattcaaag tatttaaaag agcatgctat tcttaattta
     1681 gaattaggta gaactatctc tttataatca ccaaatctgt ttggcttttg acctgcattc
     1741 tcccctatta aaattgcacc tttctcttgt tttaagaata aaagatgatg catagctgat
     1801 gagaatgttt ttctcccaat tgctacatat atctttttat tgtttgggag tttctcaaaa
     1861 agatcttcca ttacttttgt atttcctccc ctattctttc ttaaatcaac aataatatta
     1921 tcagcgtttt gtgctgaatt aagaagttta ttcttaaaag ttgttaattc ttcttttgta
     1981 tgtccttcat ttgtacattc attgtattga aaaagcagat tgttatttat taacctgaaa
     2041 tgatatttat tgttaccaac ataagtagga tcgttttttt cagacatttt ccattttaat
     2101 ggttctttaa tttttatctt ttgttcagaa tctgctttta tatcaatttc ttcattattt
     2161 gttgttaatt taacagaatc agaattacta aagttataat atcttaaaat tttgtttgag
     2221 ggtaagtatc tagataaatc acttagaaga acttctctat tttcttttga ggatagtctt
     2281 gatatttgag ggaatatttc acttaaaggg tgttcattta tttttaatat ctcatgtcca
     2341 ataatatgat tgtaattatc tgatgatcct attatgtaaa aatgtccttc aaattcttta
     2401 cattcaattg gtagataatc gttatttaat attcctttta catatgtatg ggcatctcta
     2461 gttagtgcta tagactcttg aattgcaagg ggaaagaatt cttcattttc tttttgggct
     2521 ttattaaggg aattgataaa gtcttctctg cttacattta ggtatggatt aaagtgtgtt
     2581 gcttctattt tattaactag atattctata tcttctcttt caggtgtttg ttctatttct
     2641 gtttgttcca tctggtaatt atatcaaaaa gagcaggaat aaatttttaa gtaagtaaaa
     2701 cctttttcat ctcagacatt tttattttta cactcttaag tttgaaattc ttcttttttg
     2761 taatattaaa acctaaggaa ttataaaaag gaagagcatt aagtgtagac attaattgta
     2821 tttcatttat attatttctt ctaaaatcat tttctatata ttcatatatt tgtctgccat
     2881 atccctttct ttgtttactt ggagttattt gtatttgata tatactttta tcttttttag
     2941 aatattgtgc aaatccaatt atttcattat tttctcttaa taaaatccat gttgtatttt
     3001 ttaattggtt cttaacgttc tctctgttta ttaatgaaat ccattcatca atttgtttat
     3061 tattgtaata ttgtgagttt gtttctttaa tacattttgt atgtatttct actatttttt
     3121 ctattatttt ggtatttgta ctgggagaga ttaaggtaat catggctatt tattattact
     3181 ttcttcttct agcaagtaat ttattaatct ctctatcccc cagtactcgt gtttttcctc
     3241 agggatatta ttatttgttc tttctgaggt tataagtgta ataccgtttg tattatcaaa
     3301 aaatggaata gataaagagc ttacattttc aggtagtata ttaagaattt tatcagttat
     3361 agcccttatt tcatctttat tagtagcatt tgctaaatca ttaacatatt ctattaaggg
     3421 gttattgttt atattgtttt ctataattac ttctctgttt tccatatata atattatata
     3481 aaatggtgga atatactttc aaggttggaa cttaaaagca aaaccttttt aaagtataat
     3541 ttctagtcta tgctacaagt tgttcaatta cataaggaat atgataggct ccaacttaaa
     3601 tatggagacc ctactctctc ctctatatat ggtgcaggtt gcataaataa tccagaggtt
     3661 atgtttatct ttatgaatcc tacaggtaag aatatttcta gtagccattc ttggaaggga
     3721 ttaagagctc cttggattgg tacaaaacaa gtatgggata tttttgaaaa tcttggatta
     3781 ttacaatcta agatatattt gaaaataaaa aatattagac cagaagaatg ggattatgat
     3841 tttgccaatg aggtatatac tgacatccaa aatcattcca catttattac taatcttgca
     3901 aagtgtactc aaattgatgc aaggcctctt aataattctg ttttcaaaga atacttagat
     3961 ttaatgtata aagagataga gattattaat cctaaaaaga tagttacttt tggtaatcag
     4021 gtatcttcta ttttgttaga taagaatatt tctgttagta aatatcttgg tgatgagggg
     4081 gaggtattaa atggttatga aatatatccg acatattatc ctgtaggtca aggaagaagg
     4141 aatatgcctt tagcaatagg cagattgatg aatgttttga ataaataatg gtggactata
     4201 atttcaggca tggaacttaa aatctaacta gtattaatcg ttttcttgaa ctagttctat
     4261 ttcaaaagct ccagctccga attttttttg atccttataa gaatagtatt taagcatatt
     4321 tcttgcacag tcataattgg aataatctga cggccaatta gcatctttta ttgggaaaag
     4381 taaaaataat tctttaaaat cttttgctct aataattcta agaactttta ctacgacttt
     4441 ttgatctaat tctggaagct tagaaaaggt tatagtatcc cctttggaaa tcttactcct
     4501 cttttcatca aataagcggg tttcatactt ctgtagacct gctttcacat cagcaaaagc
     4561 ctttgcattt agtttcattt catggttcat attgaataat atatcaaaaa aggaagtttt
     4621 aaaatggtgg gcgatacagg acttgaacct gtgaccttca caatgtcaat gtgacgctct
     4681 aaccagctga gctaatcgcc cgtttcattt gttaatccac tttcacttac agaatatgca
     4741 attaaatctc ctaaactcca atccctctgt tcttcttcag gaatatctgc acactctctt
     4801 tgtgctgtaa cagaaacaga atctccaaca ggtatagaaa gagatcttac attttgagga
     4861 agatgattta atgtttcttc tataactttc tttaactttt cttcttctgt tgttttactt
     4921 aatttgtcaa tttctcttac atattttaat aaaggatttt cttctcgttc tatatctctt
     4981 tccatatcat tacttactta atttaaatat atttatctct tactaataac cttatctatt
     5041 aatccatact cttttgcttc ttgtgctgta aaatatttat ctctatctgc atccttttgt
     5101 atttgtttta tactctgtcc agtttgtttt gataacattc tgtataactg atctcttgtt
     5161 tttaatatct cttctgcctc tattgcaata tcactggctt gtccttgtac tccacctaat
     5221 ggctgatgaa tatgaatttt actgtttgga agtgcaaatc tctttccctt tgctccaaat
     5281 gcaagaagaa gggaacccat tgaggcagct aatcctactg caattgttga cacatcacat
     5341 tttatatagt tcatagtatc tagtattgcc atacctgcat ttatttctcc cccaggagag
     5401 tttataaaca tctgaatatc ttcatcagga ttttcttttt ctaaaaacaa aagctgagct
     5461 actattgtat tagctaaatg tgtttcaatg ggacctccta aaaatacaat cctatcctta
     5521 agaagtctag aatatatatc atatgctctt tctccttccc tatctttttc aattactgtt
     5581 ggtattaaaa atgccatata tttaaatatt tacattttaa ttatgttcat ctatatactc
     5641 tttgcctaat acctcttcaa taaacatatt aaaagctttt tcttttcttc ctaccctctt
     5701 catattttct ctccactgtg gattagtaaa gatactttta tctacatcag ctctttgctt
     5761 tttaatttct tctattcttg catcaagctc ctcatctgtc atctgtatat ttcttgaatc
     5821 agcataaagg gtaagaaaga catctgcttc tattgcttct tttgcatcaa gcttccacaa
     5881 ctctctcatc ttttctattg taatattatt agctttaaga aaatcatcaa tattaattcc
     5941 tctgctcttc atatcttcta caaaagcttt ctctctttcc cgtgcctcat aatctattgc
     6001 ctgttgtggt atttcaatat tacactcctt tattgcaagt tttaatgcac tatcctgcat
     6061 ttgatgaatt tgataatgtt ctttttcttt taagagtgca tcttttattt tttcttttaa
     6121 agcatctaaa gatttaatct ccttctctcc tattatttct cctatttctt ttgcccattc
     6181 atcatttatt tcttttgttt ttgttttttg tgatttctta agttcattta aagcactatc
     6241 tatttcactt tgttcaactg taagcttttc ttttggaact tttacctttt tcatatcacc
     6301 aagtttaaaa gtaggcattg ttgtaacagt tattgtaaaa ggaatatcta agttttccat
     6361 gaacttagga agctctttga attcaggtgg tgcaattgga tccatgtttt cctttgttag
     6421 cacattgtta atgtataatg gggctaatct ttgaaaagtt tctaatctta aaacatcttt
     6481 tgcctcatta cttataaaat ctgcaggtac ttttcctttc ctgaaacctt taacatctaa
     6541 atctttagag tagtcttgga gcattaaatc atatgaatgc ttaaagctgt ctgcaggaat
     6601 tgtaatattc aattcatgag tatattcatt aatatctttt cttgtgtatt tagtatccat
     6661 taatatattt attgaaatta agaaatgtat tgtattctat gtgtttttat acaatttgtc
     6721 cagttttccg actataacta gtctttgctt tgaagtccat aagggagaac aagttattaa
     6781 tgtaattctg taatcagagt aatcttgaag aacagagatg tcatttttct caactacctt
     6841 tgtttctgta actatgtatg taaattgatt gtcattttga acaacctcaa tcttatctcc
     6901 tacttttacc ttatctaagt taaaaaaggt atctttattg ggaggaagct ttgcatacct
     6961 gtgtccaatt actaccatgt tgcccttctg cccaggaaga tttgaagaag ggaagtgcca
     7021 gaagcccttg ttcattgtat gagagtctgt accttcatat acaagacctt ctacatcaat
     7081 agagttaata attattttag tatttaattc ttctaatatt tttctatcaa taactgtttg
     7141 aggattaaga ctctcctcta tactttgtcc tccagaactt tgtattgcaa cacctaagac
     7201 ttcatctctt gtaggtacaa gttctgtgat aatttggaag gttatctcct ctgcactcct
     7261 ctgtacatta aatcttttta taggtccaat taatttattg taatttggat agattagaaa
     7321 gtatccaaga ggagttagta tcattaatat tagtattatt gtaactataa ttttggcact
     7381 ctgcttcatg cttttatttt atcatttaat ttaatatctt gcactatttt taattttgtt
     7441 ttatgctata cacatagact atataaattt taatataggt tggtgtggca aagaaaaaaa
     7501 atattaagaa atctgcctca aaagtatcta aaaaagaaga aagtatgaag aacaaggttg
     7561 aaataaagca accttcttct gttaagcaag aaaagctttc tagattagtt ggttctattt
     7621 ttatcggatt gggtgttctt ttagttgcat ttggaatttt ttcatatatt aaatacagag
     7681 aagagccttt gcttgatcct gagcttgaat ctcctcaaat tgagaatgtt acatcagtaa
     7741 ctaatgggga tgtaataaaa atacagggaa gtgctaaggg atatgacagt gtctttatat
     7801 atgctaatga tgagaagatt gcagaagcaa aggtagagga tgattccaag ttcagttatg
     7861 aatatcctgt tgaaaaagag ggagagtata aaattgctgt tgctggtgta aagggttttc
     7921 caagtagaaa gatatctcca aagtctgaaa ttgcgacttc aattgttgat agaacagcac
     7981 caagcaaaga aggagttaca ctcaaatatg ggacagaaac aaacaaaaac acatttatac
     8041 ttgttggtac cgttgagcct aacgcaacag tagaggtaaa aagaggaaca gatagcttta
     8101 gtgcattagc tgataaagat ggaaacttta ggattgaagg gataaaactt gaagaaggga
     8161 agaatgtgta ttctgtatac ataaaagatt tagcaggaaa tgaggtacag cttgatgaaa
     8221 aagtaagagt aaactactct cctcttggta gtgttaatgg agatgcagtt gttgatcaga
     8281 atattccaca ggcagcagga acatttgatg agttaatagg gaataattta atgatgatat
     8341 ttggtttact tgccttactt gcatttgtaa taagttcttc tgttgtatat ttgaagaata
     8401 aaagataagg ttatttgcat agaaatagaa aagagaggtg taaaagcctc tcttttttgt
     8461 ataatagaga gtatgaaaag aataattttg ttaattttat tatttattac cttctttttg
     8521 gtctctcctg tttatgctca aaaggaagtt ttctatcatg gtgtaataaa tggatataag
     8581 gagattccat gctttaaggc attagaaggt agttataaat gttttgagta ttatgtagat
     8641 atagaggagt taaatgatca gaaagttatt tctccgatat tatcagagaa tgaagagtca
     8701 agatttagga aaggagataa agtgtttgtt tcttttctta gtgatgagta tggaaatgaa
     8761 gaatggagta taacagggta tgatagaaat ggctcaatac tattaatagt ttgttgtttt
     8821 tctgctctgg ctttaattat aggtgggaag cagggctttg gatcactagt tagcttaatt
     8881 tttactgttt taatccttta tctttgggct atacctagga tacttaatgg tgctgatgtc
     8941 atatctacag ggataatttc tgtaattgta atgattgttg taattatgta ttcttcttat
     9001 ggtattaata taaagagtca tattggtgta atttcctgca taataggtgt gttaattgtc
     9061 gggctccttg cgaagttatt t
//
LOCUS       DAOSAB010000005         8671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_5929_length_8671_cov_2.660980, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000005 DAOSAB010000000
VERSION     DAOSAB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5929_length_8671_cov_2.660980"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2713)
                     /locus_tag="PK432_00230"
     CDS             complement(<1..2713)
                     /locus_tag="PK432_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59391.1"
                     /translation="MACYEIRSCQDSNVIETIDTTAVLAAGDFIKPTPAITGPGYLLN
                     CWEVLGSSAPPCVPGAFSVTSVVIYNTCPACLTAPTPCYSLTSCDGLTVLYTNANLLP
                     FIGGYVTNAIYPGQCFYVAVSQNCVGTVPFDNPQPCSCPCYSLRDCTGVIPPFNTTSN
                     LAAHVGNLVHLNQHGGCAGPCFFVSVNTGTCLVPQPVTVSTGCQPCPPCVCYRLIDCI
                     TRVPYLTINGPTLNGVDLAPLVGQVIGQVCLGPNNTQCTTGCWEIQTVQCNGNQSSVY
                     VYNISPTCQACNAVCYKLTNCVTFQEVLVEYTVPNATLPNISTLVGQTLNNPCFTGPT
                     GCLSGCWTVSLNNGPNCNGSVNWTTALSYTAFPTCNDCLPKCYLLTECAPSVAPPIVV
                     NNDLSIYVGSTVKICDSNDVCKCYDVELAQTCSGSITLDNANARFIDCDECNSCFCPP
                     GYIKVDNRCQKITSVPAVRSEKTYTATAGSVSNTYGSSGTRFYPGASSLPYPLTAVTG
                     PDRFVDASSTNVFFTSSAIGVWGGPTGSRLNTVGVWTTNAPAPLNEWIGFAKCIVNTQ
                     ATIYSIGIGGDDHIRIKLDGVIVAEATIGSFDYNFWHVFELNIQAGTHVIVVEGRNTG
                     GTAAFGAEIYQASVATVSAIAAAPALQAVTIFSTFPKRTDSSLFDTGQNSGYSCPTGY
                     TYNNCGVPSCSLIETIPYTDCPDTFRVTDCAGIQDPVITNTDLSAFLTGSYKACFTVP
                     GPWPNGCYCITVEQIDYEVGVSFPALISPTAFDCCEDCLLICYVLTDCQNAIGPFTVC
                     NDLADYVGKVIKITGCGDTCWQVSVAQSCTGNTIFGGDVIEFEDCEACLPPPPPPPVP
                     YDLDLRKIKPGYNSPNSCYTTNYIEKINCSFAEQVYNAMLVKRYGITM"
     gene            complement(2725..2913)
                     /locus_tag="PK432_00235"
     CDS             complement(2725..2913)
                     /locus_tag="PK432_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59392.1"
                     /translation="MKCTNCGSALSCGCQKRTASDGRAVCGACITNYEASLKQAKLSS
                     PGNTAPSNVNVFYTAPKK"
     gene            complement(2934..3497)
                     /locus_tag="PK432_00240"
     CDS             complement(2934..3497)
                     /locus_tag="PK432_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59393.1"
                     /translation="MARHVLSLDIPDTLNRCIFRIVDTSVYAPSVEVTCPLLQITVPG
                     FIHPINLSPPKINPGFNVNLTACDLGLQSADCGTTFYDIPDGIYIVKYSVEPKDLVYV
                     EYNHLRITCALNGIRNVYCEMNLGACAPSEPQEEKLNKLRIIQQQLQAAKAYVEDCHD
                     PKRGMELYKYAVSQLNKLTCNTNCKTC"
     gene            complement(3572..4930)
                     /locus_tag="PK432_00245"
     CDS             complement(3572..4930)
                     /locus_tag="PK432_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59394.1"
                     /translation="MFLGTGATRSAGVAGPTNPHSVGGFITTPNVPSVELSKLTTGYF
                     GFFDPNTWLSINPTLLNYNCCPLILASSSVLQNDKIGPFHGGYKETNKSKMINPKYVQ
                     KAYWVQPCVPQQAVVSVGTTPQTIIPALPAENFQGGTVQSNCCFQFLCGETYYLRIDV
                     KGSPALRALNHNAYQTLDAYTGCCAGPTPTAVDPTTVYIQWAEKVTINNYLKPFILPV
                     VFDYTQVAWYAPGTTVDPVTGAAVTSAQWWTNYPASPQAIAWTSALPNTCEAGLRLFG
                     AYVETKFGDCTFQVTDFFEKEPVKIYASMVDYTGDPCVFEGVCVYNDCLGIQGMGFGE
                     QVVRDLIKAESYLQNFFANNDLRIREITQGYDMTTAINRNALYSRYYILHSVPRWNNP
                     TSTFDNDRYMNEIIVAEANPLVPVGNATLNGFLGVWLGSCANCVPFENFGCTPCTVIP
                     NV"
     gene            complement(5047..5493)
                     /locus_tag="PK432_00250"
     CDS             complement(5047..5493)
                     /locus_tag="PK432_00250"
                     /inference="COORDINATES: protein motif:HMM:NF039553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5856 family protein"
                     /protein_id="HOK59395.1"
                     /translation="MEGIKRLNKEYSNKPGVQRESGSVDTMVAACVSELMNMATSMHK
                     LHLKVTGTGSYASHKALNELYDALPDHADTLAEGYQGAAEKLLTYKDLAPKVLNSVPE
                     ALAYIREVCDMICGLQAKMPYSEIVNSLDNVKDSLNSAKYKLLFLK"
     gene            complement(5493..6197)
                     /locus_tag="PK432_00255"
     CDS             complement(5493..6197)
                     /locus_tag="PK432_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59396.1"
                     /translation="MLNQAILIKVKQRLNKLSSNDYDNIEDWQIIEAFDKGQVDWCRR
                     NLHGLNVVKEGDEQSTRRIDDLQILLSQIPLNMNNKKTYYESVNFPPDYLQWKRVSAS
                     ATSDCCSDPKPMVIYLAEVANVDELLRDKHKQPSFEWGETFCVMSDNNVKIYTNGLFK
                     PVNVSLFYYRQPRRIQIAGVQDPYTGLPAATDVISEFKDDLVELFIDEAVKILAGDIE
                     SMNQFQRMTQSTEQNN"
     gene            complement(6197..6388)
                     /locus_tag="PK432_00260"
     CDS             complement(6197..6388)
                     /locus_tag="PK432_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59397.1"
                     /translation="MANKKYTPPSYPSKNKDAINNKIMEQFRKMKEDGANVPTVSMFN
                     LDNVPSVVPDIKNVGKSNK"
     gene            complement(6390..6566)
                     /locus_tag="PK432_00265"
     CDS             complement(6390..6566)
                     /locus_tag="PK432_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59398.1"
                     /translation="MKKLAKKEIGGASIIGSATSKARDMSTQSISKSGKAAAPAKKSI
                     SSTGAFDKYKSKKK"
     gene            complement(6569..6937)
                     /locus_tag="PK432_00270"
     CDS             complement(6569..6937)
                     /locus_tag="PK432_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59399.1"
                     /translation="MGKKVLKKAQVGFSKKSAPVDSNAYFNAAIIDAQRRANWRKAAG
                     YTSAAVNKAMEDKAAYEKKLADYKARISAKEAATKKTQQIIEEKGADSFLAEKKKTGG
                     ATKARISANSKKHVVYKKKK"
     gene            complement(6940..7158)
                     /locus_tag="PK432_00275"
     CDS             complement(6940..7158)
                     /locus_tag="PK432_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59400.1"
                     /translation="MSKGGAGVGGKKNPNTPATVVTKATSYKGGISRAPATADKAATK
                     YTGGKSTPPKGPVPSKKMGGSMKGKKAC"
     gene            complement(7195..8211)
                     /locus_tag="PK432_00280"
     CDS             complement(7195..8211)
                     /locus_tag="PK432_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59401.1"
                     /translation="MSVTIVSLADSAKSGSISIKPFFDPNKPNLGLEKYGLALFDGVF
                     HEEQLACIEKNGIKRYITGLNEFAPEIKLIPDPEVREAKIKEIRTIVAQLERELATNV
                     LDPKDPDFWNRVTLLQPNNDDFWSKISIRCGNNSVHLDPAKDPYDLIKLYAIDAGGFS
                     IVCKSFEEARSRAVPPKFYLDRFVDTVSTKTEVSKIRNKALSELIKMFDKNQNKLFYV
                     CKVVDGNSVQYKKSTPNDVMYDNMDKFITGQGVETNLRRAAQTFLDACNLDMETLKIK
                     SIVKDASFYKFINPKSDGFIYHTDSNAMMGRNVADCIEYLKNPLNDSILLDLTKKVEK
                     YWNV"
     gene            complement(8244..>8671)
                     /locus_tag="PK432_00285"
     CDS             complement(8244..>8671)
                     /locus_tag="PK432_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59402.1"
                     /translation="FRIISSGVSSSRCSKDCDSGGGDVCCDEVGSKHLKNTDWTFSML
                     RTATGVFTLRLTALNEDSYPEGLLGATFIIGATSTLGNRVTVTFNPLVTASNILEYTV
                     RTYNDAGTLSDDRLSNTLFNISIYAKPPRNRQVLPMVRF"
BASE COUNT         2738 a         1674 c         1450 g         2809 t
ORIGIN      
        1 acatggttat accgtagcgc tttacaagca ttgcattgta aacctgctct gcaaaactac
       61 agttaatctt ctcaatataa tttgttgtat aacaactatt agggctatta tatcctggtt
      121 tgatctttct tagatctaaa tcataaggaa caggaggcgg aggaggagga ggtaaacaag
      181 cttcacaatc ttcaaattca attacatctc ccccaaaaat agtattacca gtacaacttt
      241 gagcaaccga tacttgccaa catgtatcac cacaacctgt tattttaata acttttccaa
      301 cataatcagc aagatcatta catacagtaa aaggaccgat agcattttgg caatctgtta
      361 atacataaca tataagtaaa caatcttcac aacaatcaaa agctgttggt gaaattaaag
      421 ctggaaaact tacacctact tcatagtcta tttgctcaac agttatacaa taacaaccat
      481 taggccatgg accaggaaca gtaaaacaag ctttataact acccgttaaa aaagctgaaa
      541 gatcagtatt tgtaattaca ggatcttgta taccagcaca atcagttact cggaaagtgt
      601 cgggacaatc agtataagga atagtttcta tcaaagaaca actaggtact ccgcagttat
      661 tatacgtata accagtagga catgaataac cagaattctg acctgtatca aaaagactac
      721 tatcagttct ttttgggaaa gtgctaaata tagtaactgc ttgaagagca ggtgctgccg
      781 caattgcact tacagttgct acactagcct gatatatctc agctccaaaa gcagctgttc
      841 ctccggtatt tctaccttca acaactatca catgggtacc ggcttgaatg tttaattcaa
      901 acacatgcca gaagttataa tcaaaagatc caatggttgc ttcagcaact attacaccat
      961 ctaattttat tctgatatga tcatcacctc caatacctat agaataaata gtagcttgag
     1021 tattaacaat acatttagca aaacctatcc attcatttaa tggagcagga gcattagtag
     1081 tccaaacacc aaccgtgttt aatctagatc ctgtaggacc accccacaca ccaattgcac
     1141 tagatgtaaa aaatacatta gtagaagaag catcaacaaa tctatcagga cctgttactg
     1201 ctgttaaagg ataaggtaga gaagatgctc caggataaaa tcttgtacct gaagatccat
     1261 aagtattact tacactacct gctgtggcag tataagtttt ttcacttcta actgcaggaa
     1321 cactagtaat tttttgacat ctgttatcta ctttaatata accaggagga cagaagcaag
     1381 aattacattc atcacaatca ataaatcttg cattagcatt atcaagagta attgatccac
     1441 tgcatgtttg agcaagttct acatcatagc atttgcaaac atcatttgaa tcacatatct
     1501 taacagttga tcctacataa atactaagat cattatttac tacaattgga ggagccactg
     1561 aaggcgcaca ctctgttaat aaataacact taggtaaaca gtcattacaa gtaggaaatg
     1621 ctgtataact taaagcagtt gtccaattta cagaaccatt gcaattagga ccattattta
     1681 gcgagactgt ccaacatccg gataaacaac cggtaggacc agtaaagcaa ggattattta
     1741 aagtctgacc tactaaggta gagatgttgg gtagagttgc attagggact gtatattcaa
     1801 caagtacttc ctggaatgtc acacaattag taagtttata acatacagca ttacaagctt
     1861 gacaagtagg agaaatatta tatacataaa cagatgactg atttccatta cattgtacag
     1921 tctgtatttc ccaacaacct gttgtacatt gagtattatt tggaccaaga catacttgac
     1981 caattacttg tccaactaaa ggagcaagat ctacaccatt aagagtagga ccatttatag
     2041 taagataagg tactcgtgta atacaatcta ttaatctata acaaacacaa ggaggacatg
     2101 gttgacaacc agtacttact gtaaccggct gaggtactaa acaagttcca gtgttaacac
     2161 ttacaaaaaa gcaagggcca gcacatccac catgttgatt cagatgaact aaattaccta
     2221 cgtgagcagc taagttactt gttgtattaa aaggagggat aactccggta caatctctaa
     2281 gactataaca aggacaacta caaggttgag gattgtcaaa aggaactgtt cccacacaat
     2341 tttgtgaaac agctacatag aaacattgac ctggatatat tgcatttgta acataacccc
     2401 ctataaaagg aagtaaatta gcattagtat ataatacagt taatccatca caggatgtta
     2461 gtgaataaca aggtgttgga gcagttaaac aagcaggaca agtattatat ataacaacac
     2521 ttgttactga gaaagcacct ggcacacacg gaggcgcaga actaccaaga acttcccaac
     2581 agttcaggag ataacctggc cctgttatag cgggtgtagg tttgataaaa tcacccgcag
     2641 ctaatactgc agtagtgtca atggtttcta ttacattaga atcttgacaa cttcgtattt
     2701 cataacatgc catttctcaa ttaattattt tttaggagca gtgtaaaaaa catttacatt
     2761 actaggtgcg gtgttaccgg gagaacttag tttagcttgt ttaaggcttg cttcataatt
     2821 agtaatacaa gcaccacata cggctcttcc atcactagct gttctctttt gacatccaca
     2881 acttaacgct gatccacagt ttgtacattt catatttgtt ggtttttatt ggtttagcaa
     2941 gttttacaat tagtattaca agttagttta ttcaactggc taaccgcgta cttgtaaagc
     3001 tccattcctc ttttaggatc atggcaatct tctacataag ctttagcagc ttgtaactgt
     3061 tgttggataa ttcttaattt attaagcttt tcctcttgag gttctgatgg tgcacatgca
     3121 cctaagttca tttcacaata cacatttctt ataccattca aggcacatgt gatccttaga
     3181 tgattgtatt ctacatacac aagatctttt ggttcaacac tatatttaac aatgtatata
     3241 ccatccggta tatcatagaa agtagtgcca caatcagcag actgtaaacc taagtcacaa
     3301 gctgtaagat taacattaaa gcctggatta attttaggag gagaaagatt tatagggtga
     3361 ataaacccgg gaactgttat ttgtaataaa ggacaagtta cctctacact tggtgcatag
     3421 acacttgtgt caactattct aaagatacat ctattaagtg tgtccggaat atccagactt
     3481 aacacgtgtc tagccatgat gatacaaata taaagaaaaa aagggaggga gtatagactc
     3541 tcctcccctt ttaatcttga gttaataaat attatacgtt aggtattaca gtacaaggtg
     3601 tacaaccaaa gttttcaaaa ggaacacagt ttgcacaact gcctaaccat acacctaaga
     3661 atccatttaa agttgcatta cctactggaa ctaatgggtt agcttcagca acaatgatct
     3721 cattcatata gcggtcattg tcaaatgtac tagttgggtt gttccaacgt ggtacagagt
     3781 gtaagatata gtagcggcta tataatgcat tacggttaat agccgtagtc atgtcataac
     3841 cttgagtaat ctcacggata cgaagatcat tatttgcaaa gaagttttgt aagtaagact
     3901 cagccttgat taagtcacga acaacttgct caccaaagcc cataccttgg atacctaaac
     3961 agtcattgta tacacaaaca ccctcgaata cacaaggatc tccagtataa tcaaccattg
     4021 aagcatagat cttaaccggc tctttctcaa agaagtcagt tacttggaaa gtacagtcac
     4081 caaacttagt ttcaacataa gctccgaata aacgaagacc tgcttcacaa gtattaggta
     4141 atgctgaagt ccaagcaata gcttgaggag aagctggata gttagtccac cactgagctg
     4201 aagttacagc agcaccagta acaggatcta cagtagttcc tggagcatac caagcaactt
     4261 gagtatagtc aaacactact ggtaaaatga atggttttaa gtaattgtta atagttactt
     4321 tttccgccca ctgaatatat acagttgtag ggtctactgc agttggagta ggaccagcac
     4381 aacatcctgt ataagcatca agagtttggt aagcattgtg gttcaatgca cgtaatgcag
     4441 gagaaccttt aacatcaata cgtaagtagt atgtctcacc acacaagaac tggaaacagc
     4501 agttagactg tactgtacca ccttggaagt tttcagctgg aagagctgga ataattgttt
     4561 gaggagttgt tcctacagat actactgctt gttgaggaac acatggttgt acccaatatg
     4621 ctttttgtac atatttaggg ttgatcattt tagacttgtt agtctcttta taaccaccat
     4681 gaaaaggtcc aatcttgtca ttctgcaata cagaagaact agccaagatt aatggacagc
     4741 agttataatt aagtaaagtt gggttgattg acaaccaagt gtttggatca aagaatccaa
     4801 aataaccagt tgttaatttt gataattcaa cagaaggtac attaggagtc gtaataaatc
     4861 caccaactga atgaggatta gtaggtcctg ctacacctgc actgcgggtt gctcctgttc
     4921 ctaagaacat tttggagtaa gcatgattaa aataagccat tgtttttttg tttttagttt
     4981 ataaataaat aataaataat aattaaatat agtacatttt tttctctttt gcaaatataa
     5041 ttaaaattat tttaaaaaaa gtaatttata tttagcagaa ttgagactat cttttacatt
     5101 atccagagaa ttaacaattt cactgtaagg cattttagcc tgtaaaccac agatcatatc
     5161 acatacttca cggatataag ctaaagcttc tggaacactg tttaatactt ttggtgctaa
     5221 atctttgtag gtcagcagct tttccgctgc accttgatac ccttcagcta aggtatccgc
     5281 atgatcaggt aatgcatcat aaagctcatt caaagctttg tgagaagcat aagatccggt
     5341 accagttacc ttgagatgta atttatgcat agaggttgcc atgttcatca attcagaaac
     5401 acatgcagca accatagtat ctacagatcc ggattctctt tgaactccgg gcttattgct
     5461 atactctttg tttaatcttt ttataccttc cattagttat tttgttctgt tgactgggtc
     5521 attctttgga actggttcat tgattcaatg tcaccggcca ggatctttac agcctcatct
     5581 ataaataatt ctactaaatc atctttaaat tctgatatta catctgttgc tgcaggaagt
     5641 cctgtataag gatcttgtac tccagcaatc tgaattcttc taggttgtct gtaataaaat
     5701 aagcttacat taaccggttt aaataaacca ttagtataaa tctttacatt attatctgac
     5761 ataacacaga aagtctcacc ccattcaaaa cttggttgct tgtgcttatc tctaagcaat
     5821 tcatccacgt tagctacctc ggccaaatat ataaccatag gttttggatc agaacagcag
     5881 tcactagtag cacttgcaga aactcttttc cactgaagat aatcaggggg aaagtttaca
     5941 ctctcataat aagttttctt gttgttcata tttaaaggga tctgactcag taatatttgc
     6001 aagtcatcaa tcctacgggt agactgttca tctccctctt taactacgtt caatccatga
     6061 aggtttctgc ggcaccaatc aacttggccc ttatcaaaag cttcaataat ttgccagtct
     6121 tctatgttgt catagtcgtt tgacgagagc ttatttaagc gctgctttac tttaattaaa
     6181 attgcctgat taagcattac ttattacttt ttccaacatt cttaatatca ggcacaacac
     6241 ttggaacatt atccaagttg aacatgctta cagtaggtac attagcacca tcttctttca
     6301 tcttgcgaaa ttgctccatg atcttgttgt tgatagcatc cttgttctta ctaggatagc
     6361 ttggaggtgt gtatttcttg tttgccatat tatttctttt tagatttgta cttatcaaaa
     6421 gctccggtag aactaattga ttttttagca ggcgcagctg cttttccact tttactaata
     6481 ctttgtgtac tcatatctcg tgcttttgaa gttgcagaac cgattattga tgctcctcct
     6541 atttcttttt tagctaactt tttcataatt acttcttctt tttatatact acatgttttt
     6601 tactatttgc actaattcta gcttttgttg ctcctcctgt ttttttcttt tcagcaagga
     6661 aagaatcagc tcctttttcc tcaattattt gttgtgtttt ttttgttgcc gcttctttag
     6721 cagatattct agctttataa tcagcaagtt ttttttcata agctgcttta tcttccatag
     6781 ctttgtttac tgctgcactt gtgtatccag cagcctttcg ccaattagct cttctttgag
     6841 catcaataat agcagcatta aagtaagcat tactatctac tggagctgat tttttagaaa
     6901 aaccaacttg tgcttttttt aatactttct tacccattac tagcaggctt ttttaccttt
     6961 cattgatcca cccatcttct tagatggaac tggtcctttt ggaggagttg acttaccacc
     7021 agtgtatttt gtagcagctt tatctgctgt agcaggagcc ctgctgatac ctcctttata
     7081 agaagttgct tttgtaacta ctgttgctgg agtgttagga ttttttttac cacctacacc
     7141 tgctccacct ttactcattt ttttcatgat atattatttt ttaaatttac ttaattatac
     7201 attccagtac ttctctactt tcttagtaag atctaataag atagaatcat tcaaaggatt
     7261 ctttaggtac tcaatacaat ctgctacatt tctacccatc atagcattac tgtctgtgtg
     7321 gtagataaat ccatcagatt taggattgat gaatttgtag aagcttgcat ccttaactat
     7381 agacttgatc tttaatgttt ccatgtctaa gttacatgca tccaagaaag tttgagcagc
     7441 tcttcgcaag ttagtctcaa caccttgacc ggtgatgaac ttatccatgt tatcatacat
     7501 aacatcatta ggagtagact tcttgtattg aacactgtta ccatccacaa ccttgcatac
     7561 ataaaacagc ttattttggt ttttatcaaa catcttaata agctctgaca aggctttatt
     7621 gcggatcttg ctaacctcag tttttgtaga aactgtatcc acaaatctgt ctaagtaaaa
     7681 cttgggaggt actgcacggc tgcgggcttc ttcaaagctc ttacaaacta ttgagaatcc
     7741 accagcatca atagcataca atttgattaa gtcatatgga tcttttgcag gatctaagtg
     7801 tactgaatta tttccacatc taattgatat cttactccag aagtcatcat tgtttggttg
     7861 aagtaatgtt actctgttcc agaagtccgg atccttagga tcaagcacat tagtagcaag
     7921 ttctctttca agttgtgcta ctatagtacg gatttcttta attttagctt ctcttacttc
     7981 cggatctgga ataagcttaa tctcaggtgc aaattcattg agacctgtaa tgtatctctt
     8041 gataccattt ttctcaatgc atgctaattg ttcttcatga aacactccat caaataaagc
     8101 taagccatat ttctcaaggc ctaagttagg tttgttggga tcaaagaacg gcttgataga
     8161 tatactacca gatttggctg aatcagccag ggatacaatt gttacactca tgttggttta
     8221 tttttgttgg tttatattgt tttttagaat ctaaccatag gtaaaacttg gcggtttcta
     8281 ggcggtttag cataaataga tatgttaaac agtgtatttg ataatctatc atctgataaa
     8341 gttcctgcat cattgtaggt tctaactgta tattcaagga tgttagacgc agtaactaaa
     8401 ggattaaatg taacagttac tctattacct agagtagaag ttgcaccaat tataaatgta
     8461 gctcccaata aaccttctgg ataagaatct tcattcaaag ctgtaagtct aagagtgaaa
     8521 actccagttg ctgttcttag cattgaaaat gtccaatctg tgtttttcaa gtgtttacta
     8581 cctacttcat cacaacaaac atcccctcca ccagagtcac aatctttact acatctacta
     8641 gaggatactc ctgaagatat tattctgaaa g
//
LOCUS       DAOSAB010000006         8618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_5967_length_8618_cov_3.287633, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000006 DAOSAB010000000
VERSION     DAOSAB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5967_length_8618_cov_3.287633"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PK432_00290"
     CDS             complement(<1..818)
                     /locus_tag="PK432_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59403.1"
                     /translation="MRKKKYWIYDYETIINTFVAVFEDLHSNERHIFIISPYQNDLPL
                     LLAFYKENIQYQDWHLGFNNLAFDSQITEFILDNHKELSEHSNKKITKEIYNYAQDII
                     TRSDRKEFLDYPEFKLRIPVLDVYKINHWDNQNKRSSLKWIQYSMDWENIEEMPHEHY
                     KPIEDIETLNIVVEYCINDVRTTKQIFKLSLNAINLRIKLKAKYNINCLNYSNTKIGS
                     ELLLKLYCDKTGKDKKQLKEQKGITRHSINLKDIVFNYIEFKTEILQQLLKEIK"
     gene            complement(818..1114)
                     /locus_tag="PK432_00295"
     CDS             complement(818..1114)
                     /locus_tag="PK432_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59404.1"
                     /translation="MDGISKMLIYSAEWNGQPSFRLIPIMDNCPFMEVMYNPEDCILA
                     VISKTKTNIPSVKTKEGEDGEVFYEYYIESKQDILDFIKTVAVNYPHPIIEEVL"
     gene            complement(1116..1583)
                     /locus_tag="PK432_00300"
     CDS             complement(1116..1583)
                     /locus_tag="PK432_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59405.1"
                     /translation="MKNNIQKSFIFLYPLLQIQSKRSIKPLKTYMYCSGMEGELLICI
                     YKVNRKHWSSFNEKVLSKHKLLKSTVPVTDKKYAYIFDLSEFKKDYDLISVGKYHLLS
                     QFAKTCIMSFDHVNFDEVKLCLFDKQGTPPDREKETYNLSKESVEYGEFLINR"
     gene            complement(1580..2620)
                     /locus_tag="PK432_00305"
     CDS             complement(1580..2620)
                     /locus_tag="PK432_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOK59406.1"
                     /translation="MKKFTIEEGFSERIAKEEEFFSTKPYISYSGLNKLLYSPALYYK
                     HYILKQRDDINTTAAIEGKLIHCLLLTPDKFDKEYQIVSDSLPSENPKKVIDTVFEYY
                     TILKEEGDKREELIEFSSAILDVLADMNLYQSLKTDEQRIAKIVDVPKHLDYWFYLKN
                     CRGKTLITQETYEFAQGAVERALQSPVIRAALGLTIDDFNINVESYNEIELTCELTDY
                     AFNIHGILDNLVIDHDNKLIKINDFKTTNKDLTTFKESIDFYRYWMQVSFYVLLVKNS
                     IYYRENYKIEVRFLIIDCYQQVATMLVSDTTLLKWEEATKECFEKANYHLANRIFDLP
                     YEFAQCGGELII"
     gene            complement(2617..3042)
                     /locus_tag="PK432_00310"
     CDS             complement(2617..3042)
                     /locus_tag="PK432_00310"
                     /inference="COORDINATES: protein motif:HMM:NF040378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD synthetase"
                     /protein_id="HOK59407.1"
                     /translation="MNWIYDNKEILSLDDFPTNCVGFIYKITNTINGRIYVGKKALFN
                     SHRSTISKREKTITKTRKKFKVIVKESNWKTYFGSCKELQEDIKKYGEQYFVREILEF
                     CHSKKYMTYCEVKYQFKYEVLENDSYNSNIMSKFYKGNI"
     gene            complement(3062..3421)
                     /locus_tag="PK432_00315"
     CDS             complement(3062..3421)
                     /locus_tag="PK432_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59408.1"
                     /translation="MNRLKLWWYTKINTKNTIFFPNIPLGKADDTYYQFDNKKVYLYT
                     YLNDTVALKKETVTNLNLELVNHPSTLAAMRRRDLISYKNGQISHGNFGALFEYKDND
                     YLVMWDKDFIARVYKIL"
     gene            complement(3414..4400)
                     /locus_tag="PK432_00320"
     CDS             complement(3414..4400)
                     /locus_tag="PK432_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOK59409.1"
                     /translation="MINVGKYVVKADDIPADWIFSHYLKLGKLKGQSVRMKSIFNEKD
                     SVPSLIIYYNRKLNKYTFKDFSSGHSGSAIEFMRLMWKCSFSEALKRIKEDYQKSNYN
                     SEINNENIKLQGWKVTSFVVDKWKTSNIKYWNDYHITIEMLEKHKVKPLKSYTMSFVV
                     EDEIVEEFTVKNKNIYGYFYGNKEGNLAKIYQPFNKEKKFIKVSDYIQGSDQNTNSEF
                     CFIVSSLKDAMALKTIGIRGDFVCVDSENTLLPKPVITRLKRFYGESNIIVIFDNDIA
                     GISNMKKYKELYNLKFCHLPYEKDPAEILKTHGIDETRRKVVPLINKTLNNE"
     gene            complement(4411..5100)
                     /locus_tag="PK432_00325"
     CDS             complement(4411..5100)
                     /locus_tag="PK432_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59410.1"
                     /translation="MGFNVNEFNVSSDVSKVLTPGTHLCRIVDIQSGVTPFDPNKLQL
                     TFLLEGRPEGNGFIGIAIDKNDPSKGHYEGRIAYVRHGQYPFQDWERDGKVIDADTNA
                     CNFLARLAHSLGVLNEMKKDPALQKDVNIDEFVTVAKKYLCDPDLWAYYTIAGKEKWT
                     EGYNNPNYNMYFAKYENRKNYVSLKEDGVVPFDEKKHIIKTERGIPVTTETTVDVIEI
                     PNEDPFDEDPF"
     gene            complement(5121..5888)
                     /locus_tag="PK432_00330"
     CDS             complement(5121..5888)
                     /locus_tag="PK432_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK59411.1"
                     /translation="MEEKKIILPMEVAPAKIKNPRNLILFSKPKCGKTSALVQLPNSL
                     LIDLENGSDYVSGRILKANSVEDIRDIGKAIEEAGKPYKYLIIDTVTKLEELCVPYAE
                     LLYSKTLPGKNWFTKGKLEYGSILSLPNGAGYPFLWTAFEKVLGYIKTLADNVIFSGH
                     VKDKMINKAGAEFSSLDLDLTGKVARIAASDSDSIGYLYRGKGTKNYISFITSDEVAC
                     GSRSAHLRNKDILISELIDVGTENERLVTYWDKIFIE"
     gene            complement(5836..6438)
                     /locus_tag="PK432_00335"
     CDS             complement(5836..6438)
                     /locus_tag="PK432_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59412.1"
                     /translation="MSVAHDYVQDFIEFWDNYFVENNIDRSKFTTRLIFKPKEWDGEK
                     QGITFFESEMPKEGETLYIELVTTDINGKYIPNVKNNCRRLYKLEYKPTSEYDTFNRI
                     SSKKIPYKVIGVEYEQLTLCLEKSITHIDDKEKIDNNLLKTLELQTMEDSPFSSMTIR
                     DFYCILHNVPKTRQQWLNDLIKENGRKENNITNGGCSCKN"
     gene            complement(6435..7391)
                     /locus_tag="PK432_00340"
     CDS             complement(6435..7391)
                     /locus_tag="PK432_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOK59413.1"
                     /translation="MPWRKYSDALIESMNYIERRASGDIKSLKTHWQQFNSIGLNGIE
                     WGNSYILASRPGVGKSLLIASLTRQIQELNKNQDFYILHFQFEMSGKNLATREIASAN
                     NMNLRYLLSAKDKNMIDLTSEDLSKVKNYVSKQANRKEYIIDVPMTVREMKATLYAFY
                     QFVKKPFVVTLDHTMLVKKDASETSKQQTMENLAAMTIETKKALPITWFILSQLNRDI
                     DSAERQIPGKLSNFPCDADIYQSDAFMQGADVVIAYNRPAKYQLKYYGPESYVLELSD
                     KYLIAAHILKNRFGELGIHWYKADYAKMNFLEVDPPPKKEKK"
     gene            complement(7443..8120)
                     /locus_tag="PK432_00345"
     CDS             complement(7443..8120)
                     /locus_tag="PK432_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59414.1"
                     /translation="MDLEEIIEKLQDKVRTTSWYNTLRWFLMSNDFKKIITELFEFTK
                     QERRFTPGIKDLFTPFVICDYNKLKVVFLFDSPYKKVGWSNGTPIACPNTVKKTSEFK
                     YFENAVGRSLKRDMSDFAEGEVLLLHSCLTTDIIKEGSLLHYQIWKPFIIYLLDKLSN
                     EKKYIFVFVGKKAKEYAYLVDDSNPKFYLDDIPNTFDFSIDWDYKNIFNEIDKLLVKN
                     NQLPIKW"
     gene            complement(8124..>8618)
                     /locus_tag="PK432_00350"
     CDS             complement(8124..>8618)
                     /locus_tag="PK432_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59415.1"
                     /translation="CLKFLIYKDMKKKLIIDEKIEKLNKEIIEQIVNMVNSVEEKELR
                     LERDLEDKPYYIKKVTSTEVIMKSQSLFSSFKTILLEDVPFFILLDIMDCCEFYLDHY
                     QNQKEYEKNAILLFLKGYKHHDLTACDRDFRILMAKAKKKYPHFIFYEQRELLIRDWL
                     GYYN"
BASE COUNT         2859 a         1330 c          872 g         3557 t
ORIGIN      
        1 tcttttatct cttttaataa ttgttgtaat atttctgttt taaattctat ataattaaat
       61 acaatatctt ttaaattaat agaatgtctt gtaattcctt tttgttcttt taattgtttt
      121 ttatcttttc cagttttatc acagtataat tttaataata gttcactacc aatttttgta
      181 ttagaataat ttaaacaatt tatattatat tttgctttta attttattct taaatttata
      241 gcatttaaac taagtttaaa tatttgttta gtagttcgta catcattaat acaatattca
      301 actactatat ttaatgtttc tatatcttct attggtttat aatgctcatg tggcatttct
      361 tctatatttt cccaatccat tgaatattgt atccatttta atgaacttct tttattttga
      421 ttatcccagt gattaatttt atatacatct agtactggta ttctcaactt aaattcagga
      481 taatctaaaa attcttttct atctgatcta gtaattatgt cttgtgcata attataaatt
      541 tcttttgtta tttttttatt tgagtgttct gataattcct tatgattatc aagaataaat
      601 tctgttattt gactatcaaa agctaaatta ttaaacccca aatgccaatc ttgatattga
      661 atattttctt tataaaatgc taaaagcaat ggtaagtcat tttgataagg actaattata
      721 aaaatatgtc tttcatttga atgtaaatcc tcaaacactg ctacaaatgt attgattatt
      781 gtttcataat catatatcca atatttcttt tttctcatta taatacctct tctattatag
      841 gatgaggata attaactgct acagtcttta taaaatctaa aatatcttgt ttagattcta
      901 tgtaatattc atagaagact tctccatctt ctccttcttt tgtctttact gaaggtatat
      961 ttgttttagt tttagaaatt acagctaata tacaatcttc tggattatac ataacttcca
     1021 taaatggaca attatccatt attggaatta atctaaaact tggttgtcca ttccattctg
     1081 cagaatatat taacatcttg cttattccat ccatattatc tatttatcaa aaattcacca
     1141 tattctacac tctctttaga taagttataa gtttcctttt ctctatctgg tggtgtacct
     1201 tgtttatcaa aaagacataa tttaacttca tcaaaattca catgatcaaa actcattata
     1261 caagttttag caaattgact tagtaagtga tacttaccaa ctgaaattaa atcataatct
     1321 tttttaaatt ctgataaatc aaatatataa gcatattttt tatcagttac tggtacagta
     1381 ctttttaata atttatgttt ggataagact ttttcattaa aactactcca atgtttacga
     1441 tttactttat aaatgcatat taataattca ccttccatgc cactacaata catgtaggtt
     1501 tttaaaggtt ttatgcttct tttactttgt atttgtaaca aaggataaag aaatataaat
     1561 gatttctgaa tgttattttt catataatca attctcctcc acattgtgca aactcataag
     1621 gcaaatcaaa aattctattt gctaaatgat aatttgcttt ttcaaaacat tcttttgttg
     1681 cctcttccca tttcaacaag gtagtgtcac ttactaacat ggttgcaact tgttgataac
     1741 aatctataat taaaaatcta acttcaattt tataattttc tctataatat atagagtttt
     1801 ttactaataa cacataaaaa gatacttgca tccaatatct ataaaaatct atagattctt
     1861 taaatgtagt taaatcttta tttgtagttt taaagtcatt tattttaatc aatttattat
     1921 catgatctat aactaaatta tctaatatac catgtatatt aaaagcataa tcagttaatt
     1981 cacaagttag ttctatttca ttataacttt ctacattgat attaaaatca tcaatggtta
     2041 gtcctaatgc tgctcttata acaggagatt gtaatgctct ttcaacagct ccttgtgcaa
     2101 actcataagt ttcttgtgtt ataagagttt tacctctaca attttttaaa taaaaccaat
     2161 aatctaaatg tttaggaaca tcgacaattt tagcaattct ttgttcatct gtttttaaag
     2221 attggtataa attcatatct gctaacacat caaggatagc actactaaat tcaattaact
     2281 cttctctttt atcaccttct tctttgagta tagtataata ctcaaataca gtatctatta
     2341 cttttttagg attttcacta ggtaatgaat cagatacaat ttgatattct ttatcaaact
     2401 tatcaggagt taacaataaa caatggatta atttaccttc tattgctgct gtagtattaa
     2461 tatcatctct ttgttttaaa atataatgtt tataatataa agcaggacta tataatagtt
     2521 tatttaatcc agaataagaa atataaggct ttgtactaaa aaattcttct tctttagcaa
     2581 ttctttctga aaatccttct tctatagtga attttttcat atattacctt tatagaattt
     2641 tgacataatg ttactattat aactatcatt ttcaagtact tcatacttaa attgatattt
     2701 aacttcacaa tatgtcatat atttcttaga atgacaaaat tctaatattt ctctcacaaa
     2761 atactgttca ccatattttt taatatcttc ttgtaattct ttacaagaac caaaatatgt
     2821 tttccaatta gattctttta caattacttt aaattttttt ctagtttttg taattgtttt
     2881 ctctctttta gaaatagtag atctatgtga attaaaaagt gctttcttac ccacatagat
     2941 tctaccattt attgtatttg taattttata aataaatcct acacaatttg taggaaaatc
     3001 gtcaagagat aatatctctt tgttgtcata aatccaattc ataaaagttt tttatgcaaa
     3061 gttacaatat tttgtaaact cttgcaataa aatctttatc ccacattact aaataatcat
     3121 tatccttata ttcaaacaat gcaccaaaat taccatgaga tatttgacca ttcttataag
     3181 aaattaaatc tcttcttctc atagcagcta gagtagaagg atgatttact aactctaaat
     3241 ttaagttagt aactgtttct ttttttaaag caacggtatc attaagatat gtataaagat
     3301 atactttttt attatcaaac tgataataag tatcatcagc tttacctaat ggtatattag
     3361 gaaaaaatat agtatttttg gtattaatct tggtatacca ccataatttt aatctattca
     3421 ttgtttaatg ttttatttat taaaggaact acttttcttc ttgtttcatc aataccatgt
     3481 gtttttagta tttctgctgg atctttttca taaggtaaat gacaaaattt taaattataa
     3541 agttctttat attttttcat attacttata cctgctatgt cattgtcaaa aataacaata
     3601 atgttactct ctccataaaa tcttttcaga cgtgttatga caggttttgg taataaggta
     3661 ttctcactat caacacatac aaaatctcct ctaattccta ttgtttttaa tgccatcgca
     3721 tcttttaatg atgatacaat aaaacaaaat tcagaattag tattttgatc agaaccttga
     3781 atataatcag aaacttttat aaattttttt tctttattaa aaggttgata tattttagca
     3841 agattacctt ctttattacc ataaaaataa ccataaatat ttttattttt aacagtaaat
     3901 tcttctacta tttcgtcttc tacaacaaaa ctcattgtat aagattttag aggtttaact
     3961 ttatgttttt ctagcatttc aatagtaata tgataatcat tccaatattt aatattagaa
     4021 gttttccatt tatctacaac aaaacttgta actttccaac cttgtaattt aatattttca
     4081 ttatttattt cgctattata attacttttt tgataatctt cttttattct tttcagagct
     4141 tcactaaaag aacatttcca catcaatctc ataaattcaa tagcagagcc tgaatgacct
     4201 gatgaaaaat ctttaaaggt atatttgttt aattttctat tatagtatat aataagagaa
     4261 ggaacagagt ccttctcatt aaatatactt ttcattctaa cactttgacc tttaagtttt
     4321 cctaatttca aataatgact aaatatccaa tctgcaggaa tatcatcagc tttaactaca
     4381 tattttccta catttatcat attttaatct ttaaaaagga tcttcatcaa aaggatcttc
     4441 attaggtatt tcaataacat caactgttgt ttctgttgtt actggtatac ctctttctgt
     4501 ttttataata tgtttttttt catcaaatgg aactacacca tcttctttca aagatacata
     4561 attcttccta ttctcatact tagcaaaata catattataa ttaggattgt tatatccctc
     4621 tgtccatttt tctttacctg ctatagtgta ataagcccat aaatctggat cacacaaata
     4681 ttttttagca acagttacaa attcgtcaat atttacatct ttttgcaaag ctggatcttt
     4741 tttcatctca tttaatactc ctaatgaatg agctaaacga gctaaaaaat tacaagcatt
     4801 ggtatcagca tcaattactt taccatctct ttcccaatct tggaaaggat attgaccatg
     4861 tcttacataa gcaattctac cttcataatg accttttgaa ggatcatttt tgtcaatagc
     4921 aatacctata aaaccattac cttcaggtcg accttctaat agaaatgtta attgtaattt
     4981 gtttggatca aaaggtgtta ctccagattg aatatctact attctacaca aatgtgttcc
     5041 aggagttaac actttactta catcactact aacattaaat tcatttacat taaatcccat
     5101 ataattattt atttatttgt ttattctata aaaattttat cccaatatgt taccaatctt
     5161 tcattctctg tacccacatc tattaattca gatattaaaa tatctttatt tcttaaatga
     5221 gcacttctag aaccacaagc tacttcatca cttgtgataa atgagatata gttctttgtt
     5281 cctttacctc tatataaata tccaatagaa tcactatcag aagctgcaat acgagctact
     5341 ttacctgtta aatctaaatc taaagaacta aattcagcac cagctttatt tatcattttg
     5401 tccttaacat gcccagagaa aataacatta tcagctaatg ttttaatata acctaatact
     5461 ttttcaaaag cagtccataa aaaaggatat cctgccccat taggtaagga taatatactt
     5521 ccatattcta atttaccttt agtaaaccaa tttttaccag gtagtgtttt tgaatataac
     5581 aattcagcat aagggacaca cagttcttca agcttggtta cagtatctat aataagatat
     5641 ttataaggtt ttcctgcttc ttctatcgct ttaccaatat ctctaatatc ttctactgag
     5701 ttagctttta aaattcttcc tgatacataa tcagagccat tttctaaatc aataagaaga
     5761 ctattgggta attgtactaa agcagatgtc ttaccacatt ttggtttact aaaaagtata
     5821 agatttctgg gatttttaat ttttgcagga gcaacctcca ttggtaatat tattttcttt
     5881 tcttccattc tctttaatta aatcatttaa ccattgttgt ctcgtctttg ggacattgtg
     5941 taaaatacaa taaaaatctc ttattgtcat agaactaaaa ggactatctt ccatagtctg
     6001 taattctaat gttttaagta aattattatc tattttttct ttatcatcta tgtgtgttat
     6061 agatttctct aaacatagtg ttaattgttc atactctact cctatgactt tatagggtat
     6121 tttttttgat gatatacgat taaacgtatc atattcagaa gtgggtttgt attctaattt
     6181 gtataaacgt ctacaattgt tttttacatt tggtatgtat tttccattta tatcagttgt
     6241 aactaattca atatacaatg tttccccttc ttttggcatt tcactttcaa agaaagttat
     6301 accttgtttt tctccatccc attcttttgg tttgaagatt aatctagttg taaatttaga
     6361 cctgtctata ttattttcta caaaataatt gtcccaaaat tctataaaat cttgtacgta
     6421 atcatgtgct acactcattt cttttctttt ttaggtggtg gatctacttc taaaaaattc
     6481 atctttgcat aatcagcttt ataccaatgt atccctaatt ctccaaatct gttttttaat
     6541 atgtgtgctg ctattaaata tttatcagat agttctaaaa catatgattc tggaccataa
     6601 tattttaatt gatatttcgc aggtctattg tatgctataa caacatcagc accttgcata
     6661 aaagcatctg attgataaat atcagcatca catggaaaat tggataattt acctggtatt
     6721 tgtctttcag cactatcaat atctctattt aattgtgata aaataaacca agtaataggt
     6781 aaagcctttt tagtttcaat agtcattgct gctaaatttt ccatagtctg ctgtttactg
     6841 gtttctgaag catccttttt aacaagcata gtatggtcta atgtaacaac aaatggtttt
     6901 ttaacaaatt gataaaaagc atataatgta gctttcattt ctctaacagt cattggaaca
     6961 tctattatat attcttttct attagcttgt tttgaaacat aattttttac ttttgataaa
     7021 tcttcagaag ttaaatctat catattttta tctttagcag acaaaaggta tcttaaattc
     7081 atattatttg cagaagctat ttctctagta gctagatttt ttcctgacat ttcaaattgg
     7141 aaatgtaaaa tatagaaatc ttgatttttg tttaattctt gtatttgtct tgttaaagat
     7201 gcaattagta gtgattttcc taccccaggt cttgaagcaa gaatataaga gttgccccat
     7261 tctataccat tcagtccaat agaattgaat tgttgccaat gagtttttaa agattttata
     7321 tcaccagatg cccttctttc aatataattc atactctcaa taagagcatc actatatttt
     7381 ctccaaggca ttggttttgc attatctggt atcattatga caatattaat gataagtaat
     7441 aattaccatt taataggcaa ttgattattt ttaactaata gtttatctat ttcattaaaa
     7501 atatttttat aatcccaatc tatactaaaa tcaaatgtat taggtatatc atctaaatag
     7561 aatttaggat tagaatcgtc aactaaataa gcatattctt ttgctttttt acctacaaat
     7621 acaaaaatat actttttctc atttgatagt ttatctaaca aataaataat aaaaggtttc
     7681 caaatctggt agtgtaacag acttccttct ttaattatat ctgttgttaa acaactatgt
     7741 aataataata cttctccttc tgcaaaatca gacatgtctc tttttaagct tcttccaaca
     7801 gcattttcaa aatacttaaa ttcagaagtt ttttttactg tattaggaca agctatagga
     7861 gtaccgttgg accatcctac ttttttataa ggagaatcaa ataaaaatac tacttttaat
     7921 ttattataat cacatattac aaatggcgta aataagtctt taataccagg agtaaatctt
     7981 ctttcttgtt tagtaaattc aaataattct gtaattatct ttttaaaatc attactcata
     8041 agaaaccacc tcaaggtatt ataccaagag gtagttctta ctttatcttg taatttttca
     8101 attatttctt ctaaatccat aatttaatta taatatccta accaatctct aattaacaat
     8161 tctctttgtt cataaaatat gaaatgtgga tatttttttt tagcttttgc cattaaaatt
     8221 ctgaaatctc tatcacaagc tgttaaatca tgatgtttat atccctttaa aaataataat
     8281 atggcatttt tttcatattc tttttgattt tgatagtgat ctaaataaaa ttcacaacaa
     8341 tccattatat ccaataaaat aaagaatgga acgtcttcta ataatattgt tttaaatgat
     8401 gaaaataaag attgtgattt cattattact tctgtacttg ttactttttt aatataatat
     8461 ggtttatctt ctaaatccct ctctaatctt aattctttct cttccacaga attgaccata
     8521 tttacaattt gttctataat ttctttatta agtttttcaa ttttttcatc aataattaat
     8581 ttttttttca tatctttgta tattaaaaat tttaaaca
//
LOCUS       DAOSAB010000007         6368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_8471_length_6368_cov_3.085221, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000007 DAOSAB010000000
VERSION     DAOSAB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8471_length_6368_cov_3.085221"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK432_00355"
     CDS             <1..220
                     /locus_tag="PK432_00355"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF020742.2"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOK59416.1"
                     /translation="NTHEILSYDEFKKQLKGSKGFIKAYWCGRKECEESIKYETKATT
                     RCFSNEGKGRCIYCGEESTQEWLFAISY"
     gene            complement(413..3277)
                     /gene="uvrA"
                     /locus_tag="PK432_00360"
     CDS             complement(413..3277)
                     /gene="uvrA"
                     /locus_tag="PK432_00360"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOK59417.1"
                     /translation="MNNEYIDIKGAKEHNLKDISLKIPKNKLVVLTGVSGSGKSSLAF
                     DTLYAEGQRRYVESLSSYARQFLGVMHKPDVDSITGLSPAISIDQKSTGTNPRSTVGT
                     ITEIYGYLRLLFGHIGQAYCPVCGGKIVQQNIPEIYTQIVNIISKEKENFQILSPLIK
                     NRKGEYKAMLSKLHSKGFSRVIIDGDIYHLDDVEDLRINPDVKHSIDLIVDRLSFDML
                     KENKEEFRKRVIDSLELASSLSDGEMKILIGKKEYFFSEKNTCPKCQISYPKITPASF
                     SFNAPEGACPKCSGLGVINEIDISKIYNPNLTIKEGGIFPWGNRTTKDSWTTRVLQEV
                     ARVHHFDLNTPISRYPQEIFNLIFYGKGAKEYYRIQYTNRFGRERMYDAQYEGVIPEL
                     ERLYKETNSEFRKFEIEKYMTEKICDECNGNRLKPYSLAVKIGKFNIIDMTNLPVDEL
                     EEYLNTLHLQGNKAEIAKPIIKDINSRLQFLVDVGLKYISLSRRASTLSGGESQRIRL
                     ASQIGTGLTGVLYVLDEPSIGLHPRDIDKLLTSIESLRDTGNSVIIVEHDKETMERAD
                     WIVDIGPKAGENGGKVVAQGKFEDIKKANSLTGKYLSGKLKVGQNINMNLNLNVRENK
                     KISITGVKTNNLKNITLEIPLNTLTCITGVSGSGKSSLINDTLYPALMNRKMNGRQIE
                     GIYDDIKGLEYIDKVIGIDQKPIGKTPRSNPATYTGLFTYIRDLFAQTQEARTRGYKP
                     GRFSFNVKGGRCEKCKGDGVIKIEMQFLPDMYITCDKCNGKRYNEDVLAIDYKGKNIS
                     EVLDMTVDQASSMFNDIPYIKNKLGILQDVGLGYIKLGQSALTLSGGESQRIKLAKEL
                     SKQTRGHTVYILDEPTTGLHFYDIDKLLTLLKKLVAKGNTIIVIEHNLDVIKFADWIV
                     DLGPEGGNQGGKIVAQGSVMDIIENSQSYTGQWLKKKFRE"
     gene            3375..3450
                     /locus_tag="PK432_00365"
     tRNA            3375..3450
                     /locus_tag="PK432_00365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3411..3413,aa:Arg,seq:cct)
     gene            3480..5093
                     /locus_tag="PK432_00370"
     CDS             3480..5093
                     /locus_tag="PK432_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59418.1"
                     /translation="MNKKITVDEIKPPDRKDGYPSQTPKWLRRVLEFIPGAVVWFFVL
                     SPLIFAILGWEQIFVFYISYLIIYWVYRGIKFVVGVGIGVNRMKKDMDTDFLSMCKEV
                     NEKRFNELEYIYLCPVYKEGLDISEPSFEAWSKSDIGADRIHVVVAMEEKTAENLQIP
                     NFNKLKKKYGKKFASMQYYIHPSGIEGEAAGVKGANINWAARKFVEKLEKEGKDIHNY
                     MLITCDCDLRPHPKYLSAVTYKYLTVDLPDQKYYTSAVYTLNNNIWRVPVLIRVQSTM
                     LTLVTLHNWTTEKYETIPFSSGIFSSKASFSSYVVNLKTLKDVYYWDPELGIDDTTFF
                     WNAVIRFKGEFKGEEVYLPSSSDAVENETILKSYKAFYRQQHRWGWGIVIFPTTIAAL
                     IYSKDIPLNWKLHMIFAIVKNYILFFTVVYLMTVGLDLLGVFSKKYTYSSASYNLPQA
                     MSFLLTSLLICNIFLIYYRRKLIPIPKDWPWWRNLLDCGEIALIAVQMLTFGFIPYLQ
                     AHTEMMLGKGFRKNFYATEKVQIKNNSKS"
     gene            complement(5090..>6368)
                     /locus_tag="PK432_00375"
     CDS             complement(5090..>6368)
                     /locus_tag="PK432_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59419.1"
                     /translation="KEQKSTPAYPWLIKKEIAKEEIFSLQDKKYINSWLSNNRDYIYI
                     LVNEYIPESLTYKYILYRFDINTPFWNISTNPRVILTFDFVQEPMVEILLAPNGTLGV
                     LTIVTTDNTTSYILDATKGTTLDTLTVLNINDLASYKMSWAQNNQYLMFESDTDIIAL
                     DINKQTRYLLIKKTPQNGYIWSTDKQGFFYKIEVNPEYPENNNVYGYLLTQSQMDGSE
                     SKVILSDLFFQKNQDYIIRYQKDTGSGKYLPFTNSPESTKTVGEIKDFIVNQDAKGIY
                     IRTNLASYWYDIELKKYYLVSPYVSNLIGFSPDNKKFIFKDQEGFSVFTFKKEEGDHT
                     VDIGAKLIKSIDSNAVNIHWLFNSLNISYIEDNTMYICDIDGDNKTKVLQDTNLTLIQ
                     TVSSNGEKIVTLLVSPIEQTDLHNILINSYTIH"
BASE COUNT         1866 a         1024 c         1059 g         2419 t
ORIGIN      
        1 aaatacacat gagatattat catatgatga atttaagaag cagttaaagg gtagtaaggg
       61 ctttattaaa gcatattggt gtggcaggaa agaatgtgaa gaatctataa aatatgagac
      121 aaaagcgaca accaggtgtt tttcaaatga agggaagggg agatgtattt attgcgggga
      181 agaaagtacg caggagtggt tgtttgctat ttcctattag tgaaacatag tgaaatttta
      241 ggaaggcctc ttttaaggtt tcgtttcaaa tgagtgaact tcaaacagag aatttttgaa
      301 caatagaaaa attttgtatt gttattgtgt tatttttaag agttggaata ttttacaccc
      361 tctattttcg attttgtatg aaagctggaa tgggtaagaa ggcatggaag tttcactctc
      421 taaatttctt tttcaaccac tgtcctgtat agctctgaga attttcaatt atatccatta
      481 cagatccctg tgcgactatt tttcctcctt gatttcctcc ttcaggtcca aggtcaacaa
      541 tccaatctgc aaattttatt acatctaagt tatgttctat tacaattatt gtgtttcctt
      601 ttgcaactag ctttttaagg agggttagaa gtttatctat atcatagaag tgaagtcctg
      661 tagttggttc gtcaagaata tatactgtat gtcctctagt ttgtttagat agctcttttg
      721 ccaattttat tctttgtgat tctccccctg aaagtgttag tgctgactgt ccaagcttaa
      781 tatatccaag tccgacatcc tgcagaattc ccagtttgtt tttaatgtaa gggatatcat
      841 tgaacattga ggatgcttga tctactgtca tatcaaggac ttctgatatg tttttccctt
      901 tataatctat tgcaagaaca tcttcgttat atctttttcc gttgcattta tcacaagtta
      961 tatacatatc tgggagaaac tgcatttcta ttttaattac accatctcct ttgcatttct
     1021 cgcatcttcc tccctttaca ttaaagctaa atcttcctgg cttgtatcct cttgttcttg
     1081 cttcttgtgt ttgagcaaaa aggtctctta tatatgtaaa aagacctgta taagttgctg
     1141 gattggacct tggagtcttt cctataggtt tttgatctat tcctattact ttatctatat
     1201 attctaatcc ttttatatca tcatatattc cttcaatctg tcttccattc atctttctat
     1261 tcattaaagc agggtagagt gtatcgttta ttaacgaaga cttcccgctt cctgatactc
     1321 ctgttataca tgttaatgtg ttaagaggta tttctagagt tatattcttt aaattatttg
     1381 tttttactcc agttattgat atctttttgt tttctctaac atttaaattt aaattcatat
     1441 taatattttg tcctactttt agctttcctg agaggtattt tcctgttaaa gagtttgctt
     1501 tcttaatatc ttcaaacttt ccttgagcga ctaccttgcc cccattttcg cctgcttttg
     1561 gaccaatatc tactatccaa tcagctcttt ccattgtttc tttgtcatgt tctactatta
     1621 ttacactatt tccagtatct ctaagagatt ctattgatgt cagcagctta tctatatctc
     1681 ttgggtgcag accaattgat ggttcatcta gtacatacag tactcctgta agtcccgttc
     1741 ctatttgaga agccaatctt atcctttgag attctcctcc agatagtgta cttgctcttc
     1801 ttgaaagaga tatatatttt aacccaacat caactagaaa ctgtaatctg ctatttatat
     1861 ccttaataat tggtttagca atttctgctt tgtttccttg taagtgtaaa gtgtttaagt
     1921 actcttcaag ctcatctaca ggaagattag tcatatcaat aatgttaaat tttccaattt
     1981 taacagctaa agaataaggt ttaagtctat ttccattaca ctcatcacat attttctctg
     2041 tcatgtactt ttctatttca aactttctaa attctgaatt tgtttctttg tacaatcttt
     2101 ccagttcagg tattactcct tcatactgtg catcatacat tctttccctt ccaaatctgt
     2161 ttgtatattg tatcctgtag tattcttttg ctcctttacc ataaaatatg agattaaata
     2221 tctcttgagg atatcttgat attggtgtgt taagatcaaa gtgatgcact ctggcaacct
     2281 cttgtagtac ccttgttgtc catgagtctt ttgttgttct gtttccccat ggaaatattc
     2341 ctccttcttt aatggttaga ttgggattat atatttttga tatatctatc tcatttatta
     2401 ctccaagtcc tgagcatttt ggacatgcac cctctggtgc attgaaagag aatgatgcag
     2461 gagttatctt tggatatgat atctgacatt taggacatgt gttcttttca gagaagaagt
     2521 attccttttt acctataaga atcttcatct ctccatctga gagtgatgat gcaagttcta
     2581 aggaatctat tactcttttt ctaaactcct ctttattttc tttaagcata tcaaagctta
     2641 atctatctac tatcaaatct atactgtgtt ttacatcagg atttattctt aaatcttcta
     2701 catcatctaa gtgatagata tctccatcaa tgattactct tgaaaatcct tttgaatgaa
     2761 gtttagaaag cattgccttg tattcacctt ttctgttttt tataaggggt gaaaggattt
     2821 ggaagttttc tttctcttta cttatgatat ttacaatctg agtatatatt tcaggtatat
     2881 tttgttgtac aatcttgcct ccacacacag ggcagtatgc ttgtccgata tgtccgaata
     2941 aaagccttag atatccatat atttcagtta ttgttcctac tgttgatcga gggtttgttc
     3001 ctgtactttt ctggtcaatg gagatagctg gagacagtcc tgttattgaa tcaacatcag
     3061 gtttgtgcat aactcctaaa aattgtcttg catacgatga aagagattct acatatcttc
     3121 tttgtccttc tgcatacagt gtatcaaaag caagtgatga tttcccgctt cctgaaactc
     3181 ctgtaagtac aacaagtttg tttttaggaa ttttaaggga gatatctttt agattgtgct
     3241 cttttgctcc ttttatgtct atatattcat tgttcatacg atcttaatta taccatgaaa
     3301 agcaactttc tttatatttc ttaaaaacag attttactta tgatatatca atattaatgg
     3361 tataattgga acatgcaccc gtagctcaac attggataga gcaacgcact cctaacgcgt
     3421 aggttgctgg ttcgactccg gtcgggtgca tatatgtatg cttaaatagg gaatgaatta
     3481 tgaataaaaa gattacagta gatgaaatta aaccacctga tagaaaggat ggttatcctt
     3541 ctcagacacc aaaatggcta agaagggttt tagagtttat accaggtgca gttgtttggt
     3601 tctttgttct ttcccccctt atatttgcaa tactagggtg ggagcagatc tttgtcttct
     3661 atatctccta tcttataatc tactgggtgt ataggggaat taaattcgtt gttggtgttg
     3721 gtattggggt aaatagaatg aaaaaagata tggatacaga ctttctttca atgtgtaaag
     3781 aggtaaatga gaaaagattt aatgagttag agtatatata tttatgtcct gtatacaaag
     3841 aagggcttga catctcagag ccgtcatttg aggcatggag taaaagcgat attggtgctg
     3901 ataggataca tgttgttgtt gcaatggagg aaaagactgc agaaaatctt cagataccaa
     3961 attttaataa acttaagaag aagtatggta agaaatttgc tagtatgcag tattatattc
     4021 atccttctgg tatagaagga gaggctgcag gtgttaaagg tgccaatatt aattgggctg
     4081 ctagaaaatt tgttgaaaag ctggaaaaag agggtaaaga tattcataac tatatgctta
     4141 ttacctgtga ttgtgattta aggccccatc ctaaatatct atcggctgtt acttataaat
     4201 acctaactgt tgatttgcct gatcagaagt attatacaag tgctgtttat acattaaata
     4261 acaacatatg gagagttcct gttttaataa gggtgcaatc tactatgtta actttagtta
     4321 cactacataa ctggactact gagaagtatg agacaatacc attttcaagt gggattttta
     4381 gttcaaaggc atctttctca tcatatgtag ttaatcttaa aacactaaag gatgtatatt
     4441 attgggatcc tgaattgggt attgatgata caacattctt ttggaatgct gttatcagat
     4501 ttaaaggtga gtttaaaggc gaggaggttt atcttcctag tagtagtgat gcagttgaga
     4561 atgagactat actgaagtct tacaaagcat tttatagaca gcagcataga tggggatggg
     4621 gtatagttat ttttcctaca actatagcgg ctttaatata ttctaaagat attccgctta
     4681 attggaaatt gcatatgata tttgctattg tcaaaaacta tattttgttc tttacagtag
     4741 tctatctcat gacagtaggt cttgatttac ttggtgtctt tagtaagaaa tatacttata
     4801 gctctgcatc atataattta ccccaagcaa tgagtttttt gcttacctct cttttaatat
     4861 gtaatatatt tttgatatat tacagaagaa agctgatacc aatacctaaa gactggcctt
     4921 ggtggagaaa cttactagat tgtggtgaga ttgctcttat tgctgtacag atgcttacat
     4981 ttggatttat tccatatctt caggcacata cagaaatgat gcttggaaaa ggctttagaa
     5041 agaacttcta tgctacagag aaagtacaaa ttaaaaataa ttcaaagagt tagtgtattg
     5101 tatatgagtt tattagtatg ttatgcaggt ctgtttgttc aataggggaa accagtaatg
     5161 taactatttt ttctccatta gatgatactg tttgtattag tgttaagtta gtatcctgta
     5221 agaccttggt tttattatct ccatctatat cacaaatgta catagtgttg tcctctatat
     5281 atgaaatatt tagagaatta aataaccagt gaatattaac tgcattacta tctatactct
     5341 ttattaattt agcaccaata tctactgtat gatcaccttc ttctttctta aatgtaaata
     5401 cactaaatcc ttcctgatct ttaaagataa actttttatt atctggtgaa aaccctatta
     5461 aattggatac ataaggggag actaaataat attttttaag ctcaatatca taccaatatg
     5521 atgctaagtt tgttctaata tatattccct tagcatcttg atttactata aaatccttaa
     5581 tttcaccaac tgtttttgta ctttcaggag aattagtaaa aggaagatat tttccgcttc
     5641 cagtatcctt ttgatatctt ataatataat cctgattctt ttgaaaaaac agatccgaaa
     5701 gtattacctt tgattcagat ccatccattt gtgattgagt tagaagatat ccatagacat
     5761 tattattctc tggatattct ggatttacct ctattttata gaaaaaacct tgtttatctg
     5821 tactccatat atacccgttt tgaggagttt tcttaattaa caaatatcgt gtttgtttat
     5881 taatatcaag tgcaattata tctgtatcag attcaaacat aagatactgg ttattttgag
     5941 cccagctcat tttgtatgat gcaaggtcat taatattaag tactgttaaa gtatcaagtg
     6001 tagtcccctt agttgcgtct aatatatagc tagttgtgtt atctgtagta acaatagtta
     6061 agactcctaa tgtcccattt ggagcaagga gtatctctac cattggttcc tgtacaaaat
     6121 caaaggttaa aataactctt ggattagtag atatattcca aaatggagtg ttaatatcaa
     6181 acctgtataa tatgtactta taagttaaac tttctggtat gtactcatta acgagaatgt
     6241 atatatagtc tctattattg cttaaccagc tatttatata ttttttatcc tgtaaagaga
     6301 agatttcttc ctttgctatt tccttcttta taagccaagg atatgcagga gtagattttt
     6361 gttcttta
//
LOCUS       DAOSAB010000008         6162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_8805_length_6162_cov_3.126740, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000008 DAOSAB010000000
VERSION     DAOSAB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8805_length_6162_cov_3.126740"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..667
                     /gene="rplE"
                     /locus_tag="PK432_00380"
     CDS             113..667
                     /gene="rplE"
                     /locus_tag="PK432_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOK59420.1"
                     /translation="MARLKEEYDKRIKKELKEELGYSNIMEVPTLSKIVINVGAGAAL
                     KEAEAMDEIIDLVTQISGQKPVVNKAKKAVSSFKVREGMEIGVSVILRGNRMWEFFDK
                     LVNIVLPRTKDFRGLNVSAFDGAGNYSLGIEDHTVFPEIDANNVRKIRSLEITIVTTA
                     KDDKSAKALLDKFGFPFKKDVNRS"
     gene            651..845
                     /locus_tag="PK432_00385"
     CDS             651..845
                     /locus_tag="PK432_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOK59421.1"
                     /translation="MSTEAKEYKAKQLKKKSKYKTRVYNRCELCGRSRGYLRHYGICR
                     ICFRKLASKGEIPGVKKSIW"
     gene            863..1240
                     /gene="rpsH"
                     /locus_tag="PK432_00390"
     CDS             863..1240
                     /gene="rpsH"
                     /locus_tag="PK432_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOK59422.1"
                     /translation="MNDLIADLLARIQNGIERKKESVVVPRTKINMEILRVLKTEEMI
                     KDYKECEEGINVELLYDGNEPVITTLERVSKPGQRIYIASKDITPVMNGRGISIISTS
                     KGLMSGAVAKSKKLGGELICKVW"
     gene            1245..1793
                     /gene="rplF"
                     /locus_tag="PK432_00395"
     CDS             1245..1793
                     /gene="rplF"
                     /locus_tag="PK432_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOK59423.1"
                     /translation="MSRVGLNPITIEEGVDVQIDNDKRLVVVSNDERKIEIPIPSVLD
                     VKIDGNQVLVTRKDDEKFSKSMHGTVRMLINNAIYGIKHGYEKKLELVGVGYRARMEG
                     TTLVMNLGLNHPVKFESPEGITIEVPEETKVIIKGWDKQKVGEFAAKIRAFKKPEPYK
                     GKGIRYEGEYVRRKSSKSTVTA"
     gene            1797..2120
                     /gene="rplR"
                     /locus_tag="PK432_00400"
     CDS             1797..2120
                     /gene="rplR"
                     /locus_tag="PK432_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOK59424.1"
                     /translation="MNKKDRREKRKMHIRKKISGTADVPRIFVFKSNRYFYAGAANDD
                     TSTVIMSKMSKKKAEDIKKMAKEFATNLKKKKIDKGVFDRSGYRYHGLVALFADELRN
                     NGIKI"
     gene            2131..2607
                     /gene="rpsE"
                     /locus_tag="PK432_00405"
                     /pseudo
     CDS             2131..2607
                     /gene="rpsE"
                     /locus_tag="PK432_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            2774..3217
                     /gene="rplO"
                     /locus_tag="PK432_00410"
     CDS             2774..3217
                     /gene="rplO"
                     /locus_tag="PK432_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOK59425.1"
                     /translation="MYLEDIPKRKNRKMKGKRLGRGYGSGVGGHTSTRGTKGQKSRAG
                     HKSLVFFEGGNVPFYRRMPKFAGFNKPNKVEATVINLDILEKNFKDGETVSIDTLKKK
                     ALIAQKAVKVKILGDGKLTKKISVEGVPTSENAKEKILKAGGVIK"
     gene            3223..4494
                     /gene="secY"
                     /locus_tag="PK432_00415"
     CDS             3223..4494
                     /gene="secY"
                     /locus_tag="PK432_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOK59426.1"
                     /translation="MRMDKFIETFKRIFTTKDIKKKMLFSFIILIAYRFLAATPVTGI
                     TAEAISKLFEGSSFGSLLSTVSGGVLETASIAAIGLSPYINASIILQLLGTVIPKLEE
                     LRKEGAEGRRVISMYTRLLTVPLAIMQSFVIYSTLRGFGLITQLDTLHLITMIASLTA
                     GSIIVMWFAELVSESGIGGGSSYLIFLGILAGIPGTLQNNFMLMDTFQKVVLIVITLL
                     LIMSVVYVSEAERRIRVQYSRRVRMGGTMDSYIPIKLTQFGVMPVIFAVSLLSFPQLI
                     GRFILTRDVSDTIIKITNEVMNFLDNPLYNNILTFVLIIAFAMFYLTVVFKTDELAEN
                     LQKQGGFIPGIRPGKKTSQYLRGISLRLAVIGAIFLALISILPNIMIQAGLMTTTIVS
                     GTGLLIMVSVALDIRRQVSSMIVVRDYSRYL"
     gene            4517..5164
                     /locus_tag="PK432_00420"
     CDS             4517..5164
                     /locus_tag="PK432_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOK59427.1"
                     /translation="METILFHGPSGSGKDTQVDLLVQRYEFESIGTGEMFRSMYKEGD
                     IESIKAYQYWSKGKLVPNDLVYSMLNRWVKRFDTTKSWAFVSVVRDVGQISLFDDLLT
                     KNNRTLNAFVHFLLSEDAAIERMSLRKICPNCDRTYHEKYKPEKVKGYCDKCGTKLVQ
                     RVDDQPEKIKIRLKEYNETIGPIVETYKNRGILIEIDASPSIEEIHTQLVQKLGL"
     gene            5304..5540
                     /gene="infA"
                     /locus_tag="PK432_00425"
     CDS             5304..5540
                     /gene="infA"
                     /locus_tag="PK432_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOK59428.1"
                     /translation="MAVDTKKVYEFEGVVKKCLPDTKFIVEIEVNGSKREVLGYLSGK
                     MRMHYIRILEGDKVKIEMTPYDKTQGRIIYRMMQ"
     gene            5541..5657
                     /gene="rpmJ"
                     /locus_tag="PK432_00430"
     CDS             5541..5657
                     /gene="rpmJ"
                     /locus_tag="PK432_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOK59429.1"
                     /translation="MKVKASVKKRCAGCYTVTRKGRVYVYCKKNPKHKQRQG"
     gene            5678..>5834
                     /gene="rpsM"
                     /locus_tag="PK432_00435"
     CDS             5678..>5834
                     /gene="rpsM"
                     /locus_tag="PK432_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOK59430.1"
                     /translation="MARVAGVEIPNEKKLWVSLMSIYGIGKSLALEICKETNIDENTI
                     VKDLSESD"
     assembly_gap    5835..5844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5845..6065
                     /gene="rpsM"
                     /locus_tag="PK432_00440"
                     /pseudo
     CDS             5845..6065
                     /gene="rpsM"
                     /locus_tag="PK432_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
     gene            6073..>6162
                     /locus_tag="PK432_00445"
     CDS             6073..>6162
                     /locus_tag="PK432_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295451.1"
                     /note="located on the platform of the 30S subunit, it
                     bridges several disparate RNA helices of the 16S rRNA;
                     forms part of the Shine-Dalgarno cleft in the 70S
                     ribosome; interacts with S7 and S18 and IF-3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOK59431.1"
                     /translation="MATTKKSKRNVSSGVATVWATFNNTIISIA"
BASE COUNT         2324 a          671 c         1362 g         1795 t
ORIGIN      
        1 aagaaaagaa ggaagaaaag aaggaagaaa agaaggaaga aaagaaggaa gaaaagaaag
       61 aagttgagaa aactgtaaaa agtaaatcta aatcaaaaac taaaaaagaa taatggcaag
      121 attaaaggaa gaatatgaca aaagaataaa gaaagagtta aaagaggaac taggatattc
      181 aaatattatg gaggttccta ctttaagcaa gattgtgatt aatgtaggag caggagctgc
      241 attaaaagag gctgaagcaa tggatgagat tattgattta gttacacaga tttcaggaca
      301 gaagcctgtg gttaataaag caaaaaaggc agtatcctca tttaaggtta gagagggaat
      361 ggaaataggt gtttcagtta ttttaagagg caatagaatg tgggagttct ttgataaatt
      421 ggtaaatatt gttcttccaa ggacaaagga tttcagggga ttgaatgtat ctgcatttga
      481 tggtgctggg aattattcat tgggtattga agatcataca gtatttcctg aaatagatgc
      541 taataatgta agaaagataa gaagtttaga gataactatt gttactactg caaaagatga
      601 taagagtgct aaagcacttt tggataaatt tggttttcct tttaagaaag atgtcaacag
      661 aagctaaaga atacaaagca aaacaattaa agaagaaaag taaatacaaa acaagagtgt
      721 ataacagatg cgagttgtgc ggcaggtcaa gaggatattt aagacattat ggtatctgta
      781 gaatatgttt tagaaaattg gcaagtaagg gagaaatccc tggagttaag aaatctatat
      841 ggtaatttaa tatatttaaa agatgaatga tttaattgca gaccttttag caagaataca
      901 aaatggaata gaaagaaaga aggagtcagt tgttgttcct agaacaaaga taaatatgga
      961 gattctaagg gtattaaaga ctgaagaaat gataaaggat tataaagagt gcgaagaggg
     1021 tattaatgtt gaacttttgt atgatgggaa tgagccagtt ataactacac ttgagagagt
     1081 tagtaagcct ggtcagagga tatatattgc aagcaaggac attactccag ttatgaatgg
     1141 gagaggtatt tcaataattt ctacttccaa gggattaatg tctggtgctg ttgccaagag
     1201 taaaaagctt ggcggtgagt taatatgtaa agtttggtaa ttaaatgtct agagtaggat
     1261 tgaatccaat tacaattgaa gaaggtgttg atgtacagat tgacaatgat aaaaggttag
     1321 ttgttgtatc taacgatgaa aggaaaattg aaatccctat tccatctgta ttggatgtta
     1381 aaatagatgg gaatcaggtt cttgttacaa gaaaagatga tgagaagttt agtaaatcta
     1441 tgcatggtac tgtaagaatg ttaataaaca atgcaatata tggtataaag catggatatg
     1501 agaagaaatt agaattagtt ggagtaggat atagggcaag aatggaaggt accacattag
     1561 ttatgaattt agggcttaat catcctgtta aatttgaaag tcctgaagga ataacaatag
     1621 aggtgccaga ggaaaccaag gttataataa aaggatggga taaacaaaag gttggagagt
     1681 ttgctgcaaa aattagagca tttaagaaac cagagcctta caaaggtaaa ggtataaggt
     1741 atgagggtga gtatgtaaga agaaagagtt ctaaatcaac tgttactgca taacatatga
     1801 ataaaaaaga tagaagagaa aaaagaaaaa tgcatataag aaagaagata tcagggacag
     1861 ctgatgtacc tcgaatattt gtctttaaaa gtaacagata cttttatgca ggtgcagcaa
     1921 atgatgatac atctaccgtt attatgagta agatgtctaa aaagaaagca gaagatatta
     1981 aaaagatggc taaagaattt gcaactaatt taaaaaagaa gaaaattgat aagggtgttt
     2041 ttgatagaag tggatataga tatcatggtt tagttgcact gtttgctgat gagttaagaa
     2101 ataatggaat taagatttag ttaagtaaat atggcagaac atacaaaaga gaaaaaagaa
     2161 agagcatatg ataaaaagac agtctctata agaagggttg ctaaagtaac gaagggttca
     2221 aagagagtta gatttagtgc aatggttgtt gtaggagatc ataatggttc tgtaggagta
     2281 ggtcttggaa gaggtttaga tactagaagt gctgttgaaa aaggagagag aattgcacaa
     2341 aagaatttaa ctaaaataca gcttgcggga gatactattc ctcatgagat tattggtaag
     2401 gaaggagccg ctaaggttct tcttaaacct gcaagaccag gtactggagt tattgcaggt
     2461 tcatctgtaa gaacggtttt ggagttggcg ggaatagaca atgtgtatgg taagcttctt
     2521 ggtacaagtg attctgttgc ttctgcttat gcaacattta atgcacttaa agatttaaga
     2581 aatgggagag tattagaaag aatgaggaac atgagagaaa gagttgaact taagaaagaa
     2641 acagacaagg agaaaaagat tagagaggag aagattaaag caaagaagag attacaggag
     2701 aaagaagata ggaaattaaa agaaaagaag aatgttagaa gagtatctaa aagtaaagta
     2761 agaaagtaaa aatatgtatt tagaagatat accaaaaaga aaaaatagaa aaatgaaagg
     2821 taaaaggctt ggaagaggct atgggtctgg tgttggaggc catacttcaa caagaggaac
     2881 aaagggtcaa aaatcaagag caggtcataa atctttagtg ttttttgaag gaggaaatgt
     2941 acctttttat agaagaatgc ctaagtttgc aggatttaat aagcctaata aggtggaggc
     3001 aactgtaatt aacctagata ttcttgaaaa gaattttaaa gatggagaga cagtatcgat
     3061 agatacactt aagaaaaaag cattaattgc acaaaaggct gtgaaagtaa agattttagg
     3121 agatggaaaa ttaacaaaga agatatcagt tgaaggagta cctacttcgg agaatgctaa
     3181 agagaaaatt cttaaagcag gcggagttat aaagtaacct aaatgagaat ggacaaattt
     3241 attgagactt ttaaaaggat ctttactact aaggacatca aaaaaaagat gctcttttct
     3301 ttcattatac ttattgcata tagattttta gctgctactc ctgttacagg tattactgca
     3361 gaagctataa gtaaactttt tgaagggagt agctttggta gtttgctttc tactgtttct
     3421 ggcggggtgc ttgaaactgc atcaattgca gcaatagggt taagtccata tattaatgct
     3481 tctattatcc ttcagctttt aggtactgta attcctaaac ttgaagagct tcgaaaagag
     3541 ggtgcagagg gaagaagagt cataagtatg tatacaaggt tacttactgt tccattggca
     3601 ataatgcaat cttttgtaat atattctact cttaggggat ttggtctgat tactcagctt
     3661 gatacactac atcttataac aatgattgca tccttaacag ctggttccat aattgttatg
     3721 tggtttgcag agcttgtatc agagagtgga attggaggtg gttcttccta ccttattttc
     3781 cttggcattc ttgcaggtat tcctggtaca cttcagaaca attttatgtt aatggatact
     3841 ttccagaaag ttgtactgat agtaattact ctacttctga ttatgtctgt agtatatgtg
     3901 tctgaggcag aaagaagaat aagagtgcag tattctagaa gggttagaat gggaggtaca
     3961 atggatagct atattcccat aaaattgact cagtttggtg ttatgcctgt tatctttgca
     4021 gtatcattgc tttcttttcc tcaattaatt ggtagattta tacttacaag agatgtaagt
     4081 gatactatta taaaaattac taatgaggtt atgaattttt tagataatcc tttgtataat
     4141 aatattctta cttttgtatt aattattgct tttgcaatgt tttatcttac tgttgtattt
     4201 aaaacagatg aattagcaga aaaccttcag aagcagggag gatttattcc aggtataaga
     4261 ccgggaaaga aaacctcaca atatttaagg ggaatttcac ttagacttgc agtaattggg
     4321 gctatatttc ttgctttaat atcaatactc cctaatatta tgattcaagc aggtttgatg
     4381 acaacaacga ttgtatctgg tacaggtctc cttatcatgg taagtgttgc attggatata
     4441 agaagacagg tatcttctat gatagtcgta agggattact ctaggtattt ataatctttt
     4501 gtaatatact aaagttatgg aaactatact ttttcatggt ccatcaggaa gtggtaaaga
     4561 tacacaagta gatttgcttg ttcaaagata tgaatttgaa agcataggga caggagagat
     4621 gtttaggtct atgtataagg agggagatat tgaatcaata aaagcatacc aatactggtc
     4681 taaggggaag ctagtaccta atgatttagt ttacagtatg cttaatagat gggttaagag
     4741 atttgatacc actaaatctt gggcttttgt ttctgttgta agagatgtag gtcaaatatc
     4801 tctttttgat gatttgctga caaagaataa tagaacactt aatgcttttg ttcattttct
     4861 tttgtcagag gatgctgcaa ttgaaaggat gtctttaagg aaaatatgtc ctaactgtga
     4921 tagaacatat catgaaaaat acaaaccaga aaaagtaaaa ggatattgtg ataaatgtgg
     4981 tacaaagctt gtccaaagag ttgatgatca gccggaaaag attaaaatta ggctaaaaga
     5041 gtataatgaa acaattggac ctattgttga aacatacaaa aataggggta tattaattga
     5101 gatagatgcc tcccccagca ttgaagagat tcatacccag cttgtgcaga agttagggtt
     5161 gtaatattgc aatgtttttg atatgcttag ttcaccttta gagagtttta ggattttcga
     5221 agaaaagtat tataaaacct ttgaaaaaag ttcaaagctt tgatataatc acttcgactc
     5281 tatttatttg cagatccttg tttatggctg ttgatactaa aaaagtttat gaatttgaag
     5341 gtgtggtcaa aaaatgtcta ccagacacta agtttattgt tgaaatagag gtaaatggat
     5401 ctaaaaggga agtgttagga tatctttctg gaaagatgag aatgcactac attcgaattc
     5461 ttgaaggaga taaggtaaaa atagagatga ccccctatga taaaacacag gggagaataa
     5521 tatatagaat gatgcaataa atgaaagtta aggcttctgt aaaaaaaaga tgtgctggct
     5581 gttatactgt aacccgtaaa ggaagggtat atgtatactg taaaaagaat cctaagcaca
     5641 aacagaggca aggttaatta aaatatatat tttaaatatg gcaagagtag ctggagttga
     5701 aataccaaat gagaagaaac tgtgggtttc tttaatgagc atatatggaa taggaaaatc
     5761 tttagcatta gagatatgta aagagacaaa tattgatgag aatactattg taaaagattt
     5821 gtctgaatct gattnnnnnn nnnngtgata aggggatata ttgataagaa cttagaggtt
     5881 gaaggggaac ttagacagaa gacttttaga gatattaaaa gacttaaaga tattagaagt
     5941 tatagaggta ttctcataaa ttaggtcttc ctgtaagagg acaaaggaca agacataatg
     6001 ctcatactag aaagggtaag agcagaccag taggtggtct taagcgtaca ttagaaaaaa
     6061 cataacataa aaatggcaac tactaagaaa tcaaaaagaa atgtgtcaag tggtgtagca
     6121 actgtatggg ctacatttaa taacacaatt atatcaatag ct
//
LOCUS       DAOSAB010000009         5492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_10136_length_5492_cov_3.733309, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000009 DAOSAB010000000
VERSION     DAOSAB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10136_length_5492_cov_3.733309"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1696
                     /locus_tag="PK432_00450"
     CDS             332..1696
                     /locus_tag="PK432_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK59432.1"
                     /translation="MSLSKNLEYLRKSKKMSQEDLAFKLGVSRQAVSKWESGAAYPET
                     EKIIAICKLFDCSLDELMKDDIAELREDEKRRYTFNDLIEEVSSLLQKTVDIFNQMDR
                     KTRFRFFFELVVACIVIFFVVKIPFNYIASLGYNVFFAIGGDTAQVLKAIWRFLVDIT
                     YFITSLLIFISVYKAKFLNIANFSKKSTQTQIEGEKEEKEEGNKEEKIVRHDFGVFSF
                     LGKVFVFCLKVFVAFLSLFILFYMLVAVAGLVIVISWMFDGVFLFSLVLFALSFILLS
                     TIIIYVIYNFIINRRSKWKKVFTFLLISFIGLGISMGVGAIEMKEFTFTSSPRQDIVL
                     DKKSETFEMTDSLIIENYGYEIVYIEDEKMQDMVRVEVAYYDGFEDVEIVQNGNSIFI
                     NSRQENFSIPKIYEMLIKDLKEKEIHVDYRYLSDLYVTVYTSKENIEKIQNNIEQLHH
                     LIIE"
     gene            complement(1752..2156)
                     /gene="rplL"
                     /locus_tag="PK432_00455"
     CDS             complement(1752..2156)
                     /gene="rplL"
                     /locus_tag="PK432_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOK59433.1"
                     /translation="MAEEKKDLPKLSENAQKVIEIVEKMTVLELADLVKVMEDKFGVS
                     AAAPVAVAAPAAEAAPEEVEKSVFNIVLKDAGANKIGVIKVVREITELGLKEAKDLVE
                     AAPKVVKEGVKKEDAEAIKAKFEEAGAKVELE"
     gene            complement(2177..2707)
                     /gene="rplJ"
                     /locus_tag="PK432_00460"
     CDS             complement(2177..2707)
                     /gene="rplJ"
                     /locus_tag="PK432_00460"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOK59434.1"
                     /translation="MSKTRTQKEELLKIYKEMIKNSSGYILVSSDKIDTATITALKKD
                     LRNVNSSYTVVKNNIFKIALQESNQPLQLQEFDGATGVIFFQEDPTQPAKLIKKIQKE
                     MELLNPKCGVYQEEFLTDERVMQLADIPSREVLLSRLVGTMNAPLTGFMNAITGNIRS
                     FTILLNGISEKKANIN"
     gene            complement(2923..3141)
                     /locus_tag="PK432_00465"
     CDS             complement(2923..3141)
                     /locus_tag="PK432_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59435.1"
                     /translation="MLLRKKGEKMYKKEERHKQFPCDFCNMNECPLDGEKCPAVNLKS
                     FFKTIDPEKVYDAYEEYLKLIGLEVIEI"
     gene            complement(3246..3935)
                     /gene="rplA"
                     /locus_tag="PK432_00470"
     CDS             complement(3246..3935)
                     /gene="rplA"
                     /locus_tag="PK432_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOK59436.1"
                     /translation="MKRGKKYIKVSKDLDRTKSYSVQEGIKMAKKTSYSKFVGSLEAH
                     FDIIIPKDRDPKSIKGAVSFPYSVSDKSVTVAAFVTPDKEKEAKEAGADIVGLDSLIK
                     DIKDGKISFDVAIATPAVMPKIAVLGKELGPKGLMPNPKNGTVTDDIVSAIKEYKKGK
                     QTFACDSSGVIHIKIGKLDMDDEKLIENLHSAVSAIEEVLGKPYNQVIKRLHIAPTMG
                     PSFKIDYKKEE"
     gene            complement(3954..4379)
                     /gene="rplK"
                     /locus_tag="PK432_00475"
     CDS             complement(3954..4379)
                     /gene="rplK"
                     /locus_tag="PK432_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOK59437.1"
                     /translation="MAKPIKAIIKITIPAGQATPAPPIGPTLAPYGISTQEFCSQFNE
                     RTREGQGILTPVVITIYDDRSFDFITKTPPTSELIRRELKIKKGSERPNLDKVGKLTK
                     EQIQKIVEIKMPDLNTTDPEQAAKIIAGTAKQMGIETEI"
     gene            complement(4395..4949)
                     /gene="nusG"
                     /locus_tag="PK432_00480"
     CDS             complement(4395..4949)
                     /gene="nusG"
                     /locus_tag="PK432_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOK59438.1"
                     /translation="MVKDKKEKTNPNAKWYVLNIRTGYENSIKKELQQRIEATGMQDK
                     ILDILIPTQKKIIVKKGKQDIMDDKLFPGYVLIKMELTPQTWDIVKNTEGVRGFVKTD
                     KYPRPLPESEVKAIMKFMEVEQPSYQSSFSVGEAVKITDGAFADFIGSIQEIDNTKGK
                     VKVLISFLGREAPVELDFSQITKL"
     gene            complement(4961..5137)
                     /locus_tag="PK432_00485"
     CDS             complement(4961..5137)
                     /locus_tag="PK432_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOK59439.1"
                     /translation="MKNIFKDIINEYKQTKRLTFKELITLTAYTLVFCGIIALIILGA
                     DVLFQWGLAQFLKI"
     gene            complement(5158..5230)
                     /locus_tag="PK432_00490"
     tRNA            complement(5158..5230)
                     /locus_tag="PK432_00490"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5194..5196),aa:Trp,seq:cca)
     gene            complement(5251..5415)
                     /gene="rpmG"
                     /locus_tag="PK432_00495"
     CDS             complement(5251..5415)
                     /gene="rpmG"
                     /locus_tag="PK432_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOK59440.1"
                     /translation="MAKEKREIIALKCTECNHKNYTAYKSKNLKEKLEKKKYCKYCQK
                     HTMHVETKVK"
BASE COUNT         1643 a          869 c          822 g         2158 t
ORIGIN      
        1 cctactatat atcttgcaat acttacaatt gtagaaacaa tacaaacaga tattaaaagt
       61 tctaatacag tctgagatac cccatgatca acaatccatt gtgtaaaagc tacagaatta
      121 ttattcatat ccttaatatt ttaaagttaa agtttatgca taattatact ttgtaaagca
      181 tattattgtc gaatgatatg agtggtataa tgaaaaacag agtattttgg gtaaaatgca
      241 acccctagtt tacataaagc aactgtaagc tggtggttgc aaccctgcaa tacagataaa
      301 attgggcatt aaatttaata tataaattaa tatgagctta agtaaaaact tagagtacct
      361 aagaaaaagc aagaaaatgt ctcaagaaga tcttgctttt aaattaggtg tgtcaaggca
      421 ggcagtatct aaatgggaat ctggtgctgc atatcctgaa acagagaaaa taattgcaat
      481 atgtaagctt tttgattgtt cacttgacga acttatgaaa gatgatattg cagagttaag
      541 agaagatgag aagagaagat atacatttaa tgacttaatt gaagaggtct catctctttt
      601 acaaaagact gtagacatat ttaatcaaat ggatagaaaa acaagattta gatttttctt
      661 tgagcttgta gtagcatgta ttgttatttt ctttgttgta aaaatacctt ttaattatat
      721 tgcttcactt ggttacaatg tgttttttgc aataggggga gatacagctc aagtacttaa
      781 agctatatgg agatttttag tagatattac atattttatt acttcattac taatatttat
      841 ttctgtttat aaagcaaaat ttcttaatat tgcgaatttt agtaagaagt ctacccaaac
      901 ccaaatagaa ggagagaaag aagaaaagga ggaaggtaat aaagaagaga aaattgttag
      961 acatgatttt ggtgtatttt cttttttagg aaaagtattt gttttttgtt taaaggtttt
     1021 tgtagcattt ttatctctct ttatactttt ctatatgctt gttgccgttg caggcttagt
     1081 gattgttata tcttggatgt ttgatggtgt gtttcttttt agtttagttc tttttgcatt
     1141 atcatttatt cttctttcta ctattatcat ttatgtaatt tacaatttta taattaatag
     1201 aagaagtaaa tggaaaaaag tttttacatt tcttttaata tcctttattg gtcttggtat
     1261 aagtatggga gtaggggcta ttgaaatgaa agagtttaca tttactagtt ctcctcgtca
     1321 ggatatagtt ctggataaaa agagtgagac ttttgaaatg acagattctt taataattga
     1381 aaattatggg tatgagattg tatatattga agatgagaag atgcaggata tggtaagggt
     1441 agaagttgca tattacgatg gttttgagga tgtagaaata gttcaaaatg gaaacagtat
     1501 atttattaat tcaaggcaag aaaacttttc tattcctaaa atttatgaga tgttaataaa
     1561 agatttaaag gagaaggaaa ttcatgttga ttaccgctat ctttcagatc tttatgtaac
     1621 agtgtataca agcaaagaga atattgaaaa gatacaaaac aacatagagc aattacatca
     1681 tcttattata gagtaataat caaaaggcca cattaaagtg gccttctttt tacttcttag
     1741 aacagaggta tttattcaag ttctactttt gcacctgctt cttcaaattt agccttaata
     1801 gcctctgcat cttctttctt tactccttct tttacaacct taggagcagc ttctacaaga
     1861 tctttagctt ctttaagacc aagttctgta atctctctta caactttaat tacacctatt
     1921 ttgtttgcac ctgcatcctt aaggacgata ttgaatacag atttctcaac ttcctctggt
     1981 gcagcttctg ctgctggagc tgctactgca actggtgctg ctgcactaac tccaaatttg
     2041 tcttccatca ctttaactaa gtctgcaagt tctaaaacag tcattttttc gactatttca
     2101 ataactttct gtgcattctc acttaatttt ggaagatctt ttttctcttc tgccatttta
     2161 atatatgtta aataatttaa ttaatattag ctttcttttc agaaatacca ttcaaaagaa
     2221 tagtaaagct tcttatattc ccagtaattg cattcataaa tcctgttaat ggtgcattca
     2281 ttgttcctac aagtcttgaa agtagtactt ctcttgaagg gatgtctgct aactgcataa
     2341 ctctttcatc tgttaaaaac tcctcctgat atactccaca tttaggattt aataactcca
     2401 tttctttctg tatcttcttt attaattttg caggttgtgt gggatcttct tgaaagaata
     2461 ttactccagt agctccatca aattcttgta actgtaatgg ttgattgctt tcttgtaatg
     2521 cgattttaaa tatattgttt ttaactacag tatagctgga gtttacattt cttaagtctt
     2581 tttttagtgc agttatagtt gcggtatcta ttttatctga gcttactaag atatatccag
     2641 aactattttt tatcatttct ttatatatct taagtaactc ttctttttgt gtacgagttt
     2701 tagacatctg tatatttatt taattaattt tgaattaata tttatctcac atggatttat
     2761 ctttgtacca cgtttctttg ataaatatgt tcaggattat atattacaaa tgaagggagg
     2821 tcaagagagg atggaatagc gatacttatt tagtatatac atatactaat tttggtaatc
     2881 taccatcctc tcttgttgac agactaaagt tattatatgg agttaaattt ctataacctc
     2941 caagccaatt aacttgagat attcctcata tgcatcatag actttctcag ggtctatggt
     3001 tttgaaaaaa cttttaagat taactgctgg acatttttcc ccatcaagag gacattcatt
     3061 catattacag aagtcacaag gaaattgctt atgtctttcc tctttcttat acattttttc
     3121 tccttttttt cttaagagca aaaatagaca aatattagtc tgtcaagtaa ttattatacc
     3181 ataaaaacat ctgtttatta agtactaaca gtacttttat taacaaatat ggagagtagg
     3241 aatagttatt cttccttttt ataatctatc ttaaagctag gacccattgt aggagcaata
     3301 tgtaatctct ttattacttg gttatatggt tttcctaata cttcctctat tgctgacact
     3361 gcactatgta gattttctat taacttctca tcatccatat ctaacttccc tattttaata
     3421 tgtattacac cagatgagtc acaagcaaat gtttgttttc cttttttgta ttctttaatg
     3481 gctgaaacaa tatcatctgt tactgtacca ttcttaggat ttggcattaa acccttagga
     3541 cctaactctt tccctagtac tgcaatctta ggcattaccg caggagtagc aattgcaaca
     3601 tcaaaagaaa tctttccatc tttaatatct ttaattaaag aatccaatcc tacaatatca
     3661 gcaccagcct cttttgcttc tttttcttta tcaggagtta caaatgcagc tactgtaaca
     3721 cttttatcgc ttacagagta ggggaatgat actgcacctt taattgattt aggatctcta
     3781 tcttttggga ttataatatc aaaatgtgcc tctaggctgc ctacaaactt actgtaagaa
     3841 gttttttttg ccattttaat accttcttgt acagagtatg atttggttct atctaaatct
     3901 ttacttactt ttatatattt ctttcctctt ttcattattt taatatttat acattatatt
     3961 tctgtttcaa tacccatctg ttttgcagta cctgcaatta tttttgctgc ttgctctggg
     4021 tctgttgtgt ttaagtcagg cattttaatt tctacaatct tttgaatctg ctcttttgtt
     4081 aattttccta ccttatcgag attaggtctt tcagaccctt tctttatttt taactccctt
     4141 cttattaatt cagaagttgg gggagtttta gttataaagt caaaagatct atcatcatat
     4201 attgtaatta caacaggtgt taatattcct tgtccttctc ttgttctttc attgaactga
     4261 gaacaaaact cttgtgtaga tattccatat ggtgcaagtg taggtccaat tggaggagct
     4321 ggtgtggctt gtcctgcagg tattgttatc ttaattattg cttttattgg ttttgccata
     4381 tggttaataa aatattaaag tttagtaatc tgtgagaagt ctaattctac aggagcttct
     4441 cttccaagga aagatattaa aacttttact tttccctttg tattatcaat ttcttgtata
     4501 ctacctatga aatcagcaaa ggccccatct gtaatcttca cagcttctcc tacactaaaa
     4561 gaactttgat atgaaggctg ttcaacctcc ataaacttca ttattgcttt tacttcactt
     4621 tcaggtaagg gtcttggata tttatctgtc tttacaaatc ctcttacacc ttctgtattt
     4681 tttactatat cccatgtttg aggagtaagc tccattttaa tgagaacata gcctggaaaa
     4741 agcttatcat ccataatatc ctgctttccc ttcttaacaa taattttctt ttgtgttggt
     4801 atgaggatat ctaaaatttt atcctgcata ccagtagctt ctattctttg ctgaagctct
     4861 ttttttatac tattttcata tcctgttcta atgtttaata cataccactt agcattagga
     4921 tttgtttttt cttttttgtc tttgaccatt tatattttaa ttatatctta aggaactgtg
     4981 ctaatcccca ctgaaagagt acatctgctc ctagaattat gagtgcgatt ataccacaaa
     5041 agactaatgt atatgcagta agtgtaatta actctttaaa agttaatctc tttgtctgct
     5101 tatattcatt tattatatct ttaaatatgt ttttcatata tattgttgtt ctttacacag
     5161 gcccactccg attcgaacgg aggactacgg ttttggagac cgctgtttta ccagttgaaa
     5221 ctatgggccc taactgttaa acagcacaaa ttattttact ttggtctcca catgcatagt
     5281 atgcttttgg caatacttgc aatacttttt cttttccaat ttttctttta agtttttgct
     5341 tttgtatgca gtgtagtttt tatgattaca ctctgtacat tttagtgcta ttatttctct
     5401 tttttctttt gccattttct tgcttcctaa attaagaatg agactatata tgggaaatac
     5461 aggttattat attgaaatat tgcgaatagg tc
//
LOCUS       DAOSAB010000010         5452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_10220_length_5452_cov_3.707615, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000010 DAOSAB010000000
VERSION     DAOSAB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10220_length_5452_cov_3.707615"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PK432_00500"
     CDS             complement(<1..695)
                     /locus_tag="PK432_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOK59441.1"
                     /translation="MANLIVKGGKALNGEITPAGNKNSALPILCATLLTDEDVVINNF
                     PELTDVTKLIELMQELGSYVEWDKEKQVVKINNSGFCKSFGDAGLPLGMRGALLILAP
                     LLIRHGELKIKQEIGGCALGIREIDPHLEVFKSLGAEVKVEETIEINAKNGLVGNTLW
                     QDYMSVTTTENFIMAAARAKGVSVMINAASEPHVQDLCNFLNQMGAKISGIGSSTLSI
                     EGVDKLHGTTFTIS"
     gene            complement(701..1720)
                     /gene="murB"
                     /locus_tag="PK432_00505"
     CDS             complement(701..1720)
                     /gene="murB"
                     /locus_tag="PK432_00505"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOK59442.1"
                     /translation="MRVFKNKSLKDFNQYGVNTKAKYFIEIDSENDISSLLESEYLNE
                     ERVLILGEGNNILFVNDFNGTIIKPVIKGKKVVKEDKKNAWIKAYCGENWDKFVRWCV
                     KNNYQGIENMVDIPGNIGGAVSQSIGAYGQNIMDAVENVEAVNLKTGERTTFTNSECQ
                     FSYRSSIFKTTGNPEYMIISSTFKLNRGESNPKLNYSTVEEELKTFAQQPYTASDVMQ
                     AVINQRKKKLPSTKEYGTCGCVFENPFVTKEKYIELSKVIPNLQSYPAGEYVKIPAGR
                     VIDELGWKGKWKGNAGVYENHALCVVTNRKASGEEILKLIEEIKEEVKKKYDIDLKYE
                     INIIK"
     gene            complement(1746..2924)
                     /locus_tag="PK432_00510"
     CDS             complement(1746..2924)
                     /locus_tag="PK432_00510"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOK59443.1"
                     /translation="MLKKDIKIMITGGGSGGHLSVVKGLIDALIQDYNIPIQNIVYVG
                     GDLGMEGEKKGNSLEQKQFKDAKFKTYYIRAGKLQRKLSLNTISLLFRSILGFFDSLS
                     VVKKEKPTIIISSGGFISVPVCSAGHIFHKPIFLHEQTASVGLSNKIVGKFSKRIYVS
                     FKESIKYFPKEKTIHTGNIVRKEIFNSKATKDTDKEIVTLMVTNKQLPFIYISGGSLG
                     SHSINMKVLESLNDLLRKYRVLLQTGDNETFKDYEKAVQLKENLIDEYKERLVIKKYI
                     DGKSIGYVLNGMDIFTGRAGANTVYEIGVLKKFALFIPIPWATHNEQYLNAKVLSDIG
                     TANILEEKYLKECNLTEEIERFSNQVKLRKIDYKKLETLFPTNATYKILDDIFKSLDI
                     "
     gene            complement(2917..4098)
                     /locus_tag="PK432_00515"
     CDS             complement(2917..4098)
                     /locus_tag="PK432_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOK59444.1"
                     /translation="MSSKKRVKRKQTYDHHFPDKAVLAFVIVMAIFGTIMIYDASVYM
                     ATQEFGNQFHFLIQQLAWLLFGGIAGLIAYFVDYKKLLKFSLPFLGIIIILLALVLVV
                     GEEINFSKRWIQLGSLPSIQPAEFAKLAIVMYFSSWFAKNENKNFKKGDNKNEFTKAL
                     IPFLAVLGLVALLVLLEPDLGTTMVICITALIMFLLSGKNKEHRNRLLTLIPVIFLLG
                     IAAIVVEPYRMSRIQTYFELIFKGEVADPRGSGYQMQQILIGIGSGGFFGKGFGQSRQ
                     RFGYLVENTAFTDSTFAVLLEEFGLAGGIVMVSSWILFLTRGFKISLSARDSEGKYLA
                     AGITIWLTTQAFLNMAANVGLIPLTGIPLPFLTYGGSSTIVTVIAFALLLNVSRYSKN
                     A"
     gene            4131..4730
                     /locus_tag="PK432_00520"
     CDS             4131..4730
                     /locus_tag="PK432_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOK59445.1"
                     /translation="MTQSRKKIVIAVGGPGGSGSTTIAKMLAEHFHLKNIYAGDLFRK
                     AAKEKDYEYFEEFLQEISKGGNHLDYEIDALLQEYAQKGNVVIDSKLYGALAKTRKLK
                     CDVSIWLDAKLDVRIKRHLEKENLKGIKKLKRYLQIKRDLKKRYKIDKEKYWRLYKIK
                     YDKPALYYDIVLDTSNMNEQETFNLILKRIEDGGYIKTE"
     gene            complement(4953..5036)
                     /locus_tag="PK432_00525"
     tRNA            complement(4953..5036)
                     /locus_tag="PK432_00525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5000..5002),aa:Leu,seq:taa)
     gene            complement(5077..5152)
                     /locus_tag="PK432_00530"
     tRNA            complement(5077..5152)
                     /locus_tag="PK432_00530"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5116..5118),aa:Cys,seq:gca)
     gene            complement(5159..>5452)
                     /locus_tag="PK432_00535"
     CDS             complement(5159..>5452)
                     /locus_tag="PK432_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOK59446.1"
                     /translation="KTTQSLSRHRLNIPIWEVGNNIRRIRQNALLRGVKGYYLKDLPQ
                     DRDTAVKRAVECVYSYGELDLDDKIAIISGSSITNKSNNTILEISTVKDIIGF"
BASE COUNT         1663 a          963 c          823 g         2003 t
ORIGIN      
        1 gagattgtaa atgttgtacc atgtagttta tctactcctt ctatgcttaa tgttgaagac
       61 cctattcctg atatctttgc acccatttga tttaagaaat tacaaaggtc ttgtacatga
      121 ggttctgaag ctgcatttat cattacagaa acaccttttg ctcttgctgc tgccattata
      181 aagttttctg ttgttgttac agacatataa tcctgccaga gtgtatttcc tactaaacca
      241 ttctttgcat taatttcaat agtttcttct acttttactt ctgcaccaag acttttaaac
      301 acttctaaat gaggatctat ctcccttatg ccaagtgcgc agcctccaat ttcttgctta
      361 atttttaatt caccatgcct tattagtaat ggtgcaagta tgagtaaagc tcctctcatt
      421 ccaagtggaa ggccggcatc tccgaagctc ttgcaaaatc ctgaattatt aatttttact
      481 acttgttttt ctttgtccca ttcaacgtaa gaacccaact cctgcattaa ttcaattagt
      541 tttgtaacat cagttaactc tggaaagttg tttataacaa catcttcatc tgtaagaagt
      601 gttgcacaaa gtattggcag tgcagagttt ttattcccag caggagtaat ctccccattt
      661 aaagcttttc ccccttttac tattaagttt gccatatatt ttatttgata atattaattt
      721 catattttaa atcgatatca tatttctttt taacctcttc ttttatttct tctattaatt
      781 taagtatttc ttctcctgaa gccttcctgt ttgttactac acataaagca tggttttcat
      841 atactcctgc atttcctttc cattttcctt tccacccaag ttcatcaatt actcttccag
      901 caggtatttt tacatattct cctgcaggat atgactgaag gttaggaatt acttttgaaa
      961 gttcaatgta tttttctttt gttacaaaag gattctcaaa tacacatcca catgttccat
     1021 attctttagt tgaagggagt ttcttttttc tttgattaat aacggcctgc ataacatcag
     1081 aagctgtata cggctgttgt gcaaaagttt ttagttcttc ttcaacagta ctgtaattta
     1141 atttaggatt actttctccc ctgtttagtt taaatgtaga agagataatc atgtattctg
     1201 gatttcctgt tgttttaaat atactgcttc tatatgaaaa ttggcattcg ctatttgtaa
     1261 aagttgttct ttctcctgtt tttaagttta ctgcttctac attttcaaca gcatccataa
     1321 tattttgtcc atatgctcct atgctctgtg acacagcacc ccctatattt ccaggtatat
     1381 caaccatatt ctctatgcct tggtaattgt tctttacaca ccatctaaca aatttatccc
     1441 agttttcacc acagtatgcc tttatccaag catttttttt gtcttctttg actactttct
     1501 ttccttttat tactggttta ataatagttc cattgaaatc atttacaaac aatatattat
     1561 tcccttcacc aagtattaat actctttctt cattaagata ttctgattct aacaaagagg
     1621 aaatatcatt ttcactatct atttcaataa agtatttagc ctttgtgtta acaccatact
     1681 gattaaaatc tttaaggctt ttgtttttaa ataccctcat atactttgat tatataacat
     1741 attgattaaa tatctaaaga tttgaatatg tcatcaagaa ttttgtatgt tgcatttgtg
     1801 gggaatagtg tttctagctt tttataatct atctttctaa gttttacctg gttactaaat
     1861 ctttctattt cttctgtaag attgcactct tttaaatatt tttcttctaa tatgtttgca
     1921 gtaccaatat cgcttaatac ttttgcatta agatactgct cattatgagt tgcccaaggg
     1981 atagggataa agagggcaaa ctttttgagt acccctattt catatacagt atttgcacct
     2041 gctcttcctg taaagatatc cattccattt agaacatatc ctatactttt accatcaata
     2101 tattttttaa taactagtct ctctttgtat tcatctatta aattttcttt taactgtact
     2161 gccttctcat aatctttaaa ggtttcattg tctccagttt gaaggagtac tctgtatttt
     2221 ctaaggaggt catttaatga ttcaagaacc ttcatattaa tactgtgcga tcctaaacta
     2281 cctcctgaga tgtatatgaa aggcagctgt ttatttgtta ccataagggt aacaatttcc
     2341 ttgtctgtat ccttcgtagc cttactgtta aatatctctt ttcttacaat atttcctgta
     2401 tgtatagttt tttccttagg gaagtattta attgattctt tgaatgatac atatattctt
     2461 ttagagaatt ttcctactat tttgtttgat aatcctactg atgctgtctg ttcatggagg
     2521 aaaatcggct tatgaaaaat atgtcctgcg ctacacacag gcacagatat gaatcctcct
     2581 gatgatatta ttattgttgg tttttctttt tttacaacag atagtgaatc aaagaaacct
     2641 aaaattgatc taaatagtag ggatatggta tttaatgaga gttttctttg taattttcct
     2701 gctctaatat aatatgtttt aaattttgca tctttaaact gcttctgttc aagagagttt
     2761 cccttttttt ctccttccat tcctaaatca cctcctacat atactatatt ctgtattgga
     2821 atattgtaat cctgtattag agcatcaatt aaacccttta caacagaaag gtggcctccg
     2881 cttcctcctc cagttatcat tatttttata tcttttttaa gcatttttgc tatatctgct
     2941 tacatttaaa agtaaagcaa aagctattac tgttactatt gtactagatc ctccatatgt
     3001 tagaaatggc agcgggatac ctgttaaagg aattaatcca acattagcgg ccatattaag
     3061 aaatgcctgt gtagtaagcc atatagttat acctgctgcg agatattttc cttcactatc
     3121 tctagccgaa agtgaaattt taaaacctct tgtaagaaat aaaatccatg aagaaaccat
     3181 aactatacct cctgccaaac caaattcttc aagcagtact gcaaaagtgg aatcagtaaa
     3241 tgcagtattt tcaacaagat atccaaatcg ttgtcttgat tgtccaaaac cttttccaaa
     3301 aaatccaccg ctccctatcc cgattaatat ctgctgcatt tgataaccac ttcctcttgg
     3361 atctgcgact tcacctttaa agattaactc aaagtatgtt tgaattctag acattctgta
     3421 tggttcaact acaattgctg caataccaag aaggaaaata acgggaatta atgtgagtag
     3481 cctatttcta tgctctttgt ttttccctga aagaagaaac ataattaagg ctgtaataca
     3541 tataaccatt gttgttccta agtcaggttc aagaagtaca agtagtgcaa caagccctag
     3601 tacagctaaa aaaggaatta atgctttagt aaattcattt ttgttatcac cttttttaaa
     3661 atttttgttt tcatttttcg caaaccagct tgagaaatac atgacaatgg ctaatttagc
     3721 aaattcagca ggttgtatag agggtagact tcctaattgg atccatcttt ttgagaaatt
     3781 tatttcttcc cctacaacaa gtactaaagc aagaagaatg attataattc ctaaaaatgg
     3841 taaagagaat ttaagaagtt ttttgtaatc aacaaaatat gcaattaatc cagcaattcc
     3901 tccaaaaaga agccatgcca actgctgaat taaaaaatgg aattgattgc caaattcctg
     3961 tgttgccata tatacactcg catcatatat cataattgtt ccaaatattg ccattacaat
     4021 gacaaatgca aggactgctt tgtcaggaaa atgatgatca tatgtttgtt ttctttttac
     4081 tcttttttta ctcgacattc ttcgtttatt aatatactat atatttaact atgactcaaa
     4141 gcaggaaaaa gattgtaata gcagtcggtg gtccaggagg ctctgggagc acaactattg
     4201 caaaaatgtt agcagagcat tttcatctta aaaatattta tgcaggtgat ctttttagaa
     4261 aagctgcaaa ggagaaagat tatgaatatt ttgaggagtt tttacaggaa atttcaaagg
     4321 gaggaaatca tttagattat gaaattgatg cattactgca ggaatatgct caaaaaggga
     4381 atgtcgtaat tgatagtaaa ctgtatggag ctttagcaaa gacaagaaaa ttgaaatgtg
     4441 atgtttcaat atggttagat gctaagttag atgtaagaat taaaaggcat ttagagaaag
     4501 agaatttaaa agggattaag aaattaaaga gatatttgca gataaagcgg gatttgaaaa
     4561 aaaggtataa aatagataag gagaagtatt ggagattgta taaaataaaa tatgataaac
     4621 ctgccttgta ctatgatatt gttttagata cttctaatat gaatgagcag gagacattta
     4681 atttaatttt aaaaaggatt gaagatggag gatacattaa aacagaatga ccaagataca
     4741 tcttctacac ctcaagatat tacttcacat gttagtacaa gcccagaaaa agagtatata
     4801 gaaaataaac agaatcctga agatctagat gtaacatggt gtaggcttaa gtgtctgaaa
     4861 tgtggttatg tgtatgaagg aactgcagaa cttaagcagt gtcctaatgt ggaaataata
     4921 atcctgatat gtttgctgat cttgattagc tctgccgaga gggggaatcg aacccccact
     4981 ctattgctag aacgggattt taagtcccgc gcgtctgcct attccgccat ctcggcatgc
     5041 ttaattttat tacagtacat atactttatc aatatatgga ggtgacgatg ggattcgaac
     5101 ccatgatagt ggttttgcag accacggcct taaccaactt ggctacgtca ccaatgttct
     5161 aaaatccaat gatatctttt actgttgata tttccagtat agtattgttg gatttgtttg
     5221 taatagaact gccactaata atagcaattt tatcgtctaa atccaactcc ccatatgaat
     5281 atacacactc aacagctctt tttactgctg tgtctctgtc ttgtggaagg tctttaagat
     5341 agtaaccttt tacaccacga aggagtgcat tttgtcttat ccttcgtata ttatttccta
     5401 cttcccaaat tgggatattt aatctatgcc ttgataaact ctgtgttgtt tt
//
LOCUS       DAOSAB010000011         5447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_10234_length_5447_cov_3.128524, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000011 DAOSAB010000000
VERSION     DAOSAB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10234_length_5447_cov_3.128524"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PK432_00540"
     CDS             <1..1055
                     /locus_tag="PK432_00540"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOK59447.1"
                     /translation="TDFSFDSSKLSSDAAKLYNLIWKRAVSSQMNPEIVEDLNIYFEP
                     EAELKYKFTFLAKGQKIIFDGFRVVYGTAKEDEAIQYISNIKLADIFKKEKFLIEQKF
                     TQPPARYTEASLVKKLESLGIGRPSTYATIISTILDRKYVERERKFLFPTDIGRVVAH
                     FLKGNFTQLVDYQYTANVEDSLDEIAKGKKKYIPFLDGQYKPLMESIQKAEKNVKKED
                     VVVLGDSDEKCPVCGAEMVIKLGRYGKFLSCSRFPECKGMKDINGEGEELDFNKYLKV
                     EKCPKCGSKMVLKNGKFGKFWACENYPECKTTMPLLLNEKCPECGRNLVEKKSKWGKT
                     FKGCSGYPECKYIKKE"
     gene            1095..1730
                     /gene="radC"
                     /locus_tag="PK432_00545"
     CDS             1095..1730
                     /gene="radC"
                     /locus_tag="PK432_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOK59448.1"
                     /translation="MLPREKLQKRGVSVLSDLELVEVLVGSGVKGRDFKQISRAIMNS
                     IQRKVKDNKYLDLSDLVKIEGVGDVVAMRVLCGIELGKRIYGIFDKESTRITNSQEAY
                     EILKDMSSLKRERVDILCLNSRFECIARESIAMGSLNCANLSPREVVYSAIINNSAFV
                     ILAHNHPSGDSTPSKEDVDLTQSINKGLDMVGVQLLDHIVIGRNDWKSVNI"
     gene            1858..3009
                     /gene="rpsB"
                     /locus_tag="PK432_00550"
     CDS             1858..3009
                     /gene="rpsB"
                     /locus_tag="PK432_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOK59449.1"
                     /translation="MEKDKKEVLKKVETPKAKTENKSYKTEIPDLLTFLQAGAHFGHK
                     SSSWSPKMKKYIYEVRNGVHIIDIVKSRKLLSDALDKLGSIVDTGNVLIVGTKGQAAG
                     VIENIAKETGAFYISKRWPGGLFTNFDVIKKSVQELVKMEEKLASGGEGLVKKEILLM
                     QRNVERLNKIYEGIKFMDELPKALIVVDSMVEKNAIREARKAGIPIIALVDTNCDPDL
                     VDYPIPANDDSIKSITLYVTLFGNIIKQGKKSEALTSLRKNHLANLENLKLRYEQEKE
                     RQAKMEEDERLRMKAMREGKLTTQTTGVVRVLKKEKNIEEDFAAAEAVKAEVSSKKIE
                     DLGLSARIEKALKDAGISTVDDIGKDKEELLSIKGIGEKAVEEILKAIK"
     gene            3031..3744
                     /gene="tsf"
                     /locus_tag="PK432_00555"
     CDS             3031..3744
                     /gene="tsf"
                     /locus_tag="PK432_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013085.2"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HOK59450.1"
                     /translation="MGISIEDIKTLRAKTSAGIGLCKEALTQSNGDMQKAIEYINQRS
                     DVISRLHNLTGAKIGHCKIAFEESGKDFEKAVALIKERGWDESAEVEENTVGEGLIEY
                     YVHGKEQRLVSLVEVTCKTDFVARNDEFRAFAHEIALQVAAMKPEYISKEDVPSEKIE
                     ELKGLFKKEAESEGKPAEIVQKIIEGKLAKFYEENCLLQQKWFKDESKTIQNLLDDAI
                     SKFGEAITVRRFLVWEFGK"
     gene            3836..4381
                     /gene="frr"
                     /locus_tag="PK432_00560"
     CDS             3836..4381
                     /gene="frr"
                     /locus_tag="PK432_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOK59451.1"
                     /translation="MDIESFKVDLNKCIDDLIEDLAQIRTGRATPELVQDILINAYGT
                     QSPLKSFATINVSDAKSLIVIPWDKGITDNICKGIIAANMGFNAMAEGDHIRVTIPTL
                     TEERRKEYVKVMKERVEDARVAVRQVRQKYMQDIDEQQKAAMSEDEADRLRQEGEKLV
                     KESNAKIEEIKERKEQELMTI"
     gene            4398..>5447
                     /locus_tag="PK432_00565"
     CDS             4398..>5447
                     /locus_tag="PK432_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOK59452.1"
                     /translation="MSIIINILLLLVVISVLTFVHELGHFIAAKLVKAKVLEFSVGFG
                     PKIYSKKKNKTDFSIRILPFGGYVKILGDGDPSESKENKKDKGNLKNKSKPAQMFVML
                     AGVTMNILLAVCLYTLYLANNNWQFAIGSAYDDIHPVGAVIVKERVSDIPYMLAESGG
                     AKDSGMYEEGYIISVNGEEILDYDELTNMLENLKGETASIHACDMDNNCNSFDVLISE
                     DGKLGVYTGYNYNEYLDYSKNKLFSGFSHSINVVKLTGEALSSLMSDAKKTGDYSELS
                     NAVSGPVGIYFIVDYFKTLGLISFLGVLADLSLSLALINIFPIPALDGGRFFILLIES
                     IVRKDLDERVEAAIIN"
BASE COUNT         2043 a          620 c         1170 g         1614 t
ORIGIN      
        1 ctacagactt ctcatttgat tcatcaaaac tttcttctga tgcagctaag ctttacaatt
       61 tgatatggaa aagagcagta tcttctcaga tgaatcctga aattgttgag gatttaaata
      121 tatattttga acctgaagca gagcttaagt ataagtttac gtttttagct aagggacaaa
      181 aaataatatt tgatggattt agagtagtgt atggtacagc aaaagaagac gaagcaatac
      241 aatatatttc taatattaaa ttagcagata tatttaaaaa agagaaattc ttgattgagc
      301 agaaatttac acaaccacct gcaaggtata cagaagcatc tcttgttaag aaactagaat
      361 cgttggggat aggaaggcca tctacatatg cgacaataat ttctacgata ctagatagaa
      421 aatatgttga gagagaaagg aaattccttt ttcctactga tattggcaga gtagttgctc
      481 actttcttaa ggggaatttt acacaattgg ttgattatca atatacagcc aatgtagagg
      541 acagcttaga tgaaattgca aaaggaaaga agaagtacat tccattttta gatggtcagt
      601 ataaaccttt aatggagagt atacaaaaag cagaaaaaaa tgttaaaaaa gaggatgttg
      661 ttgttcttgg agattctgat gaaaaatgcc cagtttgtgg tgctgagatg gtaataaaat
      721 tagggagata tgggaaattt cttagctgtt caaggttccc agaatgtaag ggaatgaaag
      781 atataaatgg tgagggtgag gaactagatt ttaataagta tcttaaagtt gagaaatgtc
      841 caaaatgtgg atcaaaaatg gtgctaaaaa atgggaaatt tggtaaattc tgggcttgtg
      901 aaaactatcc cgaatgtaaa acaacaatgc ctcttctctt gaatgaaaaa tgtcctgaat
      961 gtggaagaaa tcttgttgag aaaaaaagta aatggggaaa aacatttaaa ggatgtagtg
     1021 gatatccaga gtgtaaatat attaaaaaag agtaatttaa ggatttttta atctcttttc
     1081 tttaatatta ttaagtgcta ccaagagaga aacttcaaaa aaggggggta tcagtacttt
     1141 ctgatttaga gcttgttgaa gtgcttgttg gtagcggtgt taaggggaga gattttaagc
     1201 agatatcaag agccataatg aatagtatcc agaggaaagt taaagataac aagtacttgg
     1261 atttgagcga tttggttaag atagagggag taggtgatgt tgttgcaatg agagtgctgt
     1321 gtggtattga gctaggaaag agaatttatg gtatttttga taaagagagt acaaggatta
     1381 caaattctca ggaagcatac gagattctta aggacatgag tagcttaaaa agagaaaggg
     1441 ttgatatact ttgtcttaac tcgaggtttg aatgtattgc cagagagagc attgcaatgg
     1501 gttctcttaa ctgtgcgaat ctttccccca gagaggttgt gtattctgct ataattaata
     1561 actcagcttt tgttattctt gcacacaatc atccttcagg agatagtact ccaagtaaag
     1621 aagatgttga tttaacacag agtattaaca aaggacttga tatggtagga gtgcaacttt
     1681 tagaccatat tgttataggt agaaatgact ggaaaagtgt aaatatttaa tttaacttga
     1741 aaaagtatat ataaatatat ataatcaaac tcatatttaa tttatctcat atatctctaa
     1801 tctttcctgg atagagaata tctaccctta gagatattaa aataaaagga aaaacatatg
     1861 gaaaaagata aaaaagaagt cttaaaaaag gttgaaacac ctaaagctaa gactgaaaat
     1921 aaaagctata aaacagaaat tccagatcta ttaacatttc tccaagcagg agctcatttt
     1981 gggcataaga gcagttcatg gagtcctaaa atgaaaaaat atatatatga ggtaagaaat
     2041 ggggttcaca ttattgatat tgtaaagagc agaaaactac tctcagatgc attggataaa
     2101 ctaggaagta tagtagatac aggaaatgtc ttaattgtag gaacaaaggg tcaagctgct
     2161 ggagttattg agaatatagc aaaagaaaca ggtgcttttt atatatctaa aaggtggcct
     2221 ggtggtcttt tcacaaactt tgatgtgata aaaaagagtg ttcaagaact agtaaaaatg
     2281 gaagagaagt tagcttcagg aggagaagga ttagtaaaga aggaaattct tttaatgcag
     2341 agaaatgttg aaagacttaa caagatatat gaaggtatta agtttatgga tgagcttcct
     2401 aaagctttaa ttgtagttga tagtatggtt gaaaagaatg ctattagaga agcaagaaaa
     2461 gcagggatac caataattgc tcttgttgat actaactgtg atcctgactt agtagattat
     2521 ccaatacctg caaatgatga ttcaataaag agtattacat tgtatgtaac tctctttggc
     2581 aatattatta aacagggaaa gaaatcagaa gcattaactt ctcttagaaa aaaccatcta
     2641 gcaaatcttg aaaatcttaa attacgatat gagcaagaga aagaaagaca agcaaaaatg
     2701 gaggaagatg aaaggcttag aatgaaagca atgagagaag gtaaacttac tactcaaacc
     2761 acaggtgttg taagagttct aaaaaaggag aaaaatattg aagaagattt cgccgccgca
     2821 gaagctgtta aggcagaagt ttcttcaaag aaaattgaag atcttggttt atctgcaaga
     2881 atagaaaaag cattaaaaga tgcaggaatt agtacagtag atgatatagg aaaggacaag
     2941 gaggagctat tatcaattaa aggtattggt gaaaaagcag tagaagagat actaaaagca
     3001 ataaaataat ttttgttagg agatactatt atgggtatat caatagaaga tataaaaaca
     3061 ttaagagcta aaacatctgc agggataggt ctttgtaagg aagcgctaac tcagagtaat
     3121 ggtgatatgc aaaaggcaat agaatatata aatcaaagaa gcgatgttat tagcagactt
     3181 cataatttaa caggagctaa aattggtcac tgtaagatag cttttgagga gagtggaaaa
     3241 gattttgaga aagctgttgc attaataaaa gaaagagggt gggatgaatc tgctgaggtg
     3301 gaggagaata ctgttggtga aggattaata gaatattatg tacatggtaa ggagcagaga
     3361 cttgtctctc ttgtagaagt tacttgtaag actgattttg tagcaagaaa tgatgagttt
     3421 agagcatttg cccatgagat tgctttgcag gtagctgcaa tgaagccaga atatatttca
     3481 aaagaagatg ttccttctga gaaaatagaa gaattaaagg gattatttaa aaaagaagca
     3541 gaaagtgagg gaaagcctgc agaaattgtt caaaaaatta tagaaggaaa gctagcaaaa
     3601 ttttatgaag agaactgctt attacagcag aaatggttta aggatgagtc aaaaactatt
     3661 cagaatcttc ttgatgatgc tatttctaag tttggggaag ctataactgt aaggagattt
     3721 ttagtgtggg aatttgggaa ataagaattt attataagta taaaagcccc agagtcctat
     3781 tgtgattttg tctgtttttt actattatct atatataact ttttaataaa gatatatgga
     3841 tattgaaagc tttaaagtag atcttaataa gtgtattgat gatttgattg aagaccttgc
     3901 tcagataagg acaggaagag ccacaccaga attggtacaa gatatattaa taaatgctta
     3961 tggaacacaa tcgcctctta aaagttttgc aacaataaat gtttctgatg ctaaatcatt
     4021 aattgtaatt ccatgggaca aaggaattac tgataacata tgcaagggaa taatagctgc
     4081 taatatgggt tttaatgcaa tggcagaagg agaccatatt agagtaacaa tacctacact
     4141 aacagaggaa aggagaaagg aatatgtaaa agttatgaaa gaaagggtcg aagatgctag
     4201 agtagcagtt aggcaggtaa gacagaagta catgcaagat attgatgaac agcagaaggc
     4261 agcgatgtca gaagatgagg ctgacagatt aaggcaggaa ggagaaaaat tagtgaaaga
     4321 gagtaatgca aagatagagg aaatcaaaga aagaaaagag caagaactta tgactattta
     4381 aaatattcta tacatttatg tccataatta ttaacatttt acttcttctt gttgtaatta
     4441 gtgtcttaac atttgttcat gagttagggc attttattgc agcaaagtta gtaaaagcaa
     4501 aagttctaga attctcagtg ggatttggtc ctaagatata ttctaaaaag aaaaataaaa
     4561 cagatttcag tattagaata ttacccttcg gaggatatgt aaaaatactt ggggatggag
     4621 atccgagtga gagtaaagaa aataagaaag ataagggaaa tctaaagaat aaaagtaagc
     4681 ctgctcaaat gtttgttatg cttgcaggtg taacaatgaa tatactttta gcagtatgct
     4741 tgtatacttt atatcttgct aataataatt ggcaatttgc aattggcagt gcatatgatg
     4801 atattcatcc agtaggtgct gttattgtta aagaaagggt ctcagatatt ccatatatgc
     4861 tggctgaaag tggaggtgca aaagattctg gaatgtacga agaagggtat atcatttcag
     4921 taaatggtga agagatttta gattatgatg aacttactaa tatgttggag aatctaaaag
     4981 gagagacagc ttctatacat gcatgtgata tggataataa ctgcaattct tttgatgttt
     5041 taattagtga agatggaaag cttggtgtgt atacaggtta taactataat gaataccttg
     5101 attatagtaa aaacaaacta ttctctggtt ttagtcattc aattaatgtt gttaagctaa
     5161 ctggagaggc actttcttct ttgatgtctg atgcaaagaa aacaggtgat tattccgagc
     5221 tttcaaatgc tgtgagtggg cctgttggaa tttattttat agttgattat tttaaaactt
     5281 taggtttaat ttcatttctc ggggtacttg cggatttatc tttatcactc gcacttataa
     5341 atatttttcc cattcctgca cttgatgggg gcagattctt tatacttcta attgaatcaa
     5401 tagtaaggaa ggatttagat gaaagagtag aggctgctat tattaac
//
LOCUS       DAOSAB010000012         5435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_10270_length_5435_cov_2.490892, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000012 DAOSAB010000000
VERSION     DAOSAB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10270_length_5435_cov_2.490892"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /gene="ybeY"
                     /locus_tag="PK432_00570"
     CDS             <1..384
                     /gene="ybeY"
                     /locus_tag="PK432_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOK59453.1"
                     /translation="VKQGYKEIFKGSKLENINVVFVQPKYIENLNKQYRGIDLVTDVL
                     SFNLSSGDGEIYICPAYVYESFKNSDFEEEILRLIIHGTLHILGYNHKGSLNDSSNEE
                     MFKLQEDLIIKYLAYVHSNRVRKSK"
     gene            350..907
                     /gene="pth"
                     /locus_tag="PK432_00575"
     CDS             350..907
                     /gene="pth"
                     /locus_tag="PK432_00575"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOK59454.1"
                     /translation="MFIVTGLGNPSKEFENTPHNAGYMFVNQLREFLVQESSLEINDW
                     SDEKRMFLSSICRIKKDGELIGILQKPLTYMNASGNAVKLLLNKFACDQYILAHDDLD
                     MPLGRYKIQFGKSPKSHKGVLSVESALGDKAFLRVRIGVDNRGSRIISGDEYVLMSYT
                     DKEIALLKKCIMDSILELNNNYLQL"
     gene            complement(882..2099)
                     /locus_tag="PK432_00580"
     CDS             complement(882..2099)
                     /locus_tag="PK432_00580"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOK59455.1"
                     /translation="MYLAHGAASYIVNEAVQKKKISKLNNHEQFIVAILSILFGILPD
                     IDLVILPLISTPSFTHHSTFTHGILFYLFLWLLLNLLFFVLKKILNKKGRKMLNDELL
                     NIIQYSFIISTLSHLFFDILFSYSTIFFPLEKQITILGNLIKTNYFAQYMFTPPFACE
                     AIIILFFLLILYKKYIKEGKIATKIFKLLITLSCVYLPFTIYMNLNTYNYSSHFRNGE
                     NIYDADYDRIQDSKDADTDNDGIDNIQEGDKEKIAEFAKRISTNRYFASNRQQNIWEN
                     VKYNSGAFNSYRIISQAYFEQNLAIEPVLKEYEQKKYNTNSYIQHTPSSILLYEYFTD
                     NGYLLNLDTQVESGSIFFILTNGKMVNMGIVLDSETFVTVLSTDKRVVTHTRNDIQEA
                     YPNCVIKAVNSYY"
     gene            2159..4531
                     /locus_tag="PK432_00585"
     CDS             2159..4531
                     /locus_tag="PK432_00585"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOK59456.1"
                     /translation="MGKFTQDFLKIFIPPQLNEYLAVTVGKEYEEFAAEVIGRKLSKS
                     VYMVEQKDEKILYEEYKVGDKWSVDKLLSIGYEVVERVWNEGEISILGENILIWPYSS
                     KYIFRFTLFGNVIEQIKILDPTSRNVVEKKSFARIFPKDYSTVIGNETNEEFIIIQCV
                     PNIFVDEDVADLGIRSLPYFSLASLSKSSVNILNDYKRRGYHIWYLTHDLLKYKSQEN
                     LSEYIDRIYEIEDSVEDTLKKGFLYEKGKLFVLTDAEVLGEVLLKQESSGGNIDKSSI
                     ELLKKVFVGDFVVHEDHGIGKFLGIVKKDDIEYIEIAYAGNDRLYVPLSASDKVMKYI
                     GSGKAQPVLTGLNSGVWGRVSQKAKKDVENIAKELLRIYALREISRCPKVISSPSQLD
                     EYWKFANEFEFSDTEDQYIATKKIAEDFQKDTPMDRLLVGDVGFGKTEIGMRAAFAVA
                     NSGMQVALLAPTTILANQHLKVFTERFKNYPFNIALLSRLQSDSERKEIIDSLERGKI
                     DILIGTHAILSSDVKFKELGLLIVDEEQKFGVRQKEKLKGRRIDTHVLSMTATPIPRT
                     LNLSLIGVRDISVLAIPPLGRREIKNEFFKFNFEKIKECIVRELRRNGQVYYLHNRID
                     SIYALEEKLRELIPDARIEIAHGRLRGKKLIDVMNHFINHKIDVLLCTTIIENGLDIS
                     NANTLIVDDVNRLGLAQIYQIRGRVGRGERQAYACFFYDELRGDAQLRLEAIRESQAL
                     GSGFVLSNRDLEIRGAGDILGKKQSGTINSVGYGLYTKLLNDAVEKLKRD"
     gene            4607..>5435
                     /gene="dnaX"
                     /locus_tag="PK432_00590"
     CDS             4607..>5435
                     /gene="dnaX"
                     /locus_tag="PK432_00590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOK59457.1"
                     /translation="MPTVLYRKYRAQNFDEIVAQEHITKILKNAVKSNQLSHAYLFVG
                     SRGTGKTSTARILAKAVNCMNPKKDGNPCNECEACVAITNGNYMDLVEIDAASNRGID
                     QIRELKERIEFAPTKGKYKVYIIDEVHMLTTEAFNALLKTLEEPPQHVIFILVTTDVH
                     KLPATILSRCQRYDFRLGTEEEISKTLKKILKKEDLKIDEKGLELVVRNARGSFRDAL
                     SLLDVVINSQLRSENPKEITEDEVRAVLGIPEVYMVNDFLTSLVKGDGKKALECIDEV
                     "
BASE COUNT         1863 a          631 c         1127 g         1814 t
ORIGIN      
        1 gttaaacaag ggtataaaga gatatttaaa ggaagtaaac tagaaaatat taatgttgtt
       61 tttgtacaac ctaagtatat agaaaatctt aacaaacagt atagaggaat agatttagta
      121 acagatgttc tttcttttaa tttatcttct ggggatggtg aaatatatat atgtcctgca
      181 tatgtctatg aatcatttaa gaatagtgat tttgaagagg agatcttaag attgattatt
      241 catggtacac ttcatatact tggttataat cataaaggca gtttaaatga ttcttctaat
      301 gaagaaatgt ttaaattgca agaagattta attattaaat atctcgcata tgttcatagt
      361 aacagggtta ggaaatccaa gtaaagagtt tgagaatact ccgcataatg ctgggtatat
      421 gtttgtaaat cagctaaggg aatttctagt acaggaaagt agtttagaga taaatgactg
      481 gagtgatgag aagagaatgt ttctgtcctc aatctgtagg attaaaaaag atggagaatt
      541 gattgggata ctacaaaaac ctcttacata tatgaatgct tcaggtaatg cagttaaact
      601 gcttctaaat aaattcgctt gtgatcagta tattttagct catgatgatt tagatatgcc
      661 acttggaaga tacaaaatac agtttgggaa atctcctaag agtcataaag gtgttttaag
      721 tgttgagagt gcacttggtg ataaagcatt cttaagagtt aggatagggg tagataacag
      781 agggagcagg ataatctctg gtgatgaata tgtactgatg tcatatactg ataaagaaat
      841 agcactttta aagaagtgta taatggatag tattttggaa cttaataata actatttaca
      901 gctttaatta cacagtttgg ataagcttct tgtatgtcat ttctagtgtg agttactacc
      961 cttttatctg tactaagtac agttacaaat gtttctgaat ctaaaactat ccccatattt
     1021 accatcttcc cgttagtaag tataaaaaat atactgccgc tttctacttg tgtatcaagg
     1081 ttgagtaggt atccattgtc tgtaaaatac tcgtaaagga gtatagagga gggggtatgc
     1141 tgtatatatg aatttgtgtt atatttcttt tgttcatact ctttaagaac aggttctata
     1201 gctaagtttt gctcaaagta tgcctgtgat attattctgt atgaattaaa tgcacctgaa
     1261 ttgtatttaa cattctccca aatattttgc tgtctgttag aagcaaagta cctgtttgtt
     1321 gatattctct ttgcaaattc agcaattttc tctttatctc cctcttgtat attatctata
     1381 ccatcattat ctgtgtcagc atctttacta tcttgtattc tgtcgtagtc agcatcatat
     1441 atattttctc catttctaaa gtgtgaggag taattgtatg tatttaagtt catatatatt
     1501 gtaaatggaa gatatacaca ggacagagtt attagcagtt taaatatttt agtagcaatc
     1561 tttccctcct ttatatattt tttgtatagg attagtaaga agaagaggat gattattgcc
     1621 tcgcaggcaa aaggtggtgt aaacatatat tgtgcaaaat agtttgtttt tattagattt
     1681 cctagtattg ttatctgttt ttcaagtggg aaaaatatgg tggagtaaga gaaaagtatg
     1741 tcaaagaaga gatgagaaag ggtggagata ataaaggagt attgtatgat attaagaagc
     1801 tcatcattaa gcatctttct tcctttcttg tttaggattt ttttaagaac aaagaataaa
     1861 aggtttaata gcaaccagag aaataggtaa aacaatatgc cgtgggtaaa agttgagtgg
     1921 tgagtaaatg aaggtgttga aattaatgga agtattacaa gatcaatatc aggtaagata
     1981 ccgaagagaa tacttaagat tgcaacaata aactgctcat ggttgtttag tttagaaatt
     2041 tttttctttt gtacagcctc atttactata tatgatgcag ctccatgtgc tagatacata
     2101 ctaaaaaata ttaaattaac tttttaatta tacacttcct tgataaaata tcatgtctat
     2161 gggcaaattt acacaagatt ttttaaaaat ttttatacct ccacagctta atgagtattt
     2221 agcagttaca gttggaaagg agtatgaaga atttgcagca gaagtaattg gaagaaagct
     2281 ttctaaaagt gtatatatgg tagaacaaaa ggatgaaaaa attttgtatg aggagtacaa
     2341 ggtaggagat aaatggagtg ttgataaact tttgtcaatt ggatatgagg tggttgaaag
     2401 agtatggaat gagggggaga ttagtatttt aggagagaat attttaattt ggccatatag
     2461 ttctaagtat atatttagat ttacactttt tgggaatgtc attgagcaaa ttaaaattct
     2521 tgatcctaca agtagaaatg ttgttgagaa aaagagtttt gcaaggattt tccctaagga
     2581 ttattccaca gttattggca atgaaactaa tgaggaattc attataattc agtgtgttcc
     2641 taatattttt gtagatgaag atgttgctga tttaggtatt cgttcacttc catatttttc
     2701 tcttgcctct ttaagtaaat catctgttaa tattttaaat gattataaaa ggaggggata
     2761 ccatatatgg tatcttactc atgacctttt aaagtataaa tcgcaggaaa atttgagtga
     2821 atatattgat aggatatatg agatagagga tagtgttgaa gacactttaa agaaagggtt
     2881 tttgtatgag aaaggaaaac tttttgttct tacagatgct gaagttttag gagaagtttt
     2941 gcttaaacaa gagagtagtg gggggaatat agataagtcc tcaatagagc ttttgaaaaa
     3001 ggtttttgtt ggtgattttg ttgtacatga agatcatggt atagggaagt ttcttggaat
     3061 tgttaaaaaa gatgatattg aatatattga gattgcttat gctggaaatg atagattgta
     3121 tgttccatta agtgcatctg ataaggtgat gaaatatata gggagtggaa aggctcaacc
     3181 tgttcttaca ggtttaaata gtggtgtatg gggtagggtg tctcagaaag caaagaaaga
     3241 tgtagaaaac attgctaagg agcttcttag aatatatgca ttaagagaga tatctagatg
     3301 tcctaaggtt atttcatctc cttctcaatt ggatgaatat tggaagtttg ctaatgagtt
     3361 tgagtttagt gatacagaag atcagtatat agcaacaaag aaaattgcag aggattttca
     3421 aaaggatact cctatggata gacttcttgt aggtgatgtt ggttttggta agactgagat
     3481 tggaatgaga gctgcttttg cagttgctaa tagtggtatg caagtagcac ttctagctcc
     3541 tactacaata ttagcaaatc agcatttaaa agtttttact gaaagattta agaattatcc
     3601 ttttaatatt gcattgcttt ctagattaca gagtgattct gagagaaaag aaattataga
     3661 ctcccttgag agaggaaaga tagatatatt gatagggacc catgcaattc tttcttctga
     3721 tgttaagttt aaagaacttg gtttactaat tgttgatgaa gaacagaagt ttggtgtaag
     3781 acagaaagag aaattaaagg gcagaagaat agatacacat gttctttcta tgactgctac
     3841 ccctatacca agaacattaa atctttcttt aattggtgta agagatatat cagtacttgc
     3901 aattcctccg cttggaagaa gagagattaa aaatgaattc tttaaattta attttgagaa
     3961 aattaaagag tgtattgtta gagaattaag aagaaacggg caggtatatt accttcataa
     4021 taggattgat agcatatatg cgctagagga gaaattaaga gagttaattc ctgatgcaag
     4081 aattgagatt gctcatggca ggcttagggg taaaaagttg atcgatgtaa tgaatcattt
     4141 tattaaccat aaaattgatg tattactttg tacaactata atcgaaaatg gtttagatat
     4201 atcaaatgca aacactttaa ttgttgatga tgtaaataga cttgggcttg ctcagatata
     4261 tcaaattaga gggagagtag ggagaggtga gagacaagca tatgcatgtt ttttctatga
     4321 tgagcttcga ggtgatgctc agcttagact ggaggctata agggagtctc aggccctggg
     4381 atcaggtttt gtattgtcaa atagagacct agagataagg ggagcagggg acattcttgg
     4441 aaagaaacaa agtgggacta taaattcggt ggggtatggt ttgtatacaa agcttttaaa
     4501 tgatgcagtt gaaaaattaa aaagggatta gtagtaaagg gatattgagt tgtgtaaata
     4561 attttccctt gttatagttt aatttatacc ctatcatctt atcattatgc caacagtact
     4621 gtatagaaag tatcgtgctc aaaactttga tgaaattgtc gcacaagagc acattacaaa
     4681 aattttaaag aatgctgtta agagtaatca gctttctcat gcatatcttt ttgtaggttc
     4741 aagaggtact ggtaagacta gtactgcaag aattcttgct aaggcagtta actgtatgaa
     4801 tcctaaaaaa gatggaaacc cttgtaatga atgtgaagct tgtgttgcaa ttacaaatgg
     4861 caactatatg gatcttgttg aaatagatgc tgcatcaaac agaggaattg atcagataag
     4921 agagctgaaa gagcgaattg agtttgcccc tactaaagga aagtataaag tatatattat
     4981 tgatgaggtt catatgctta caacagaggc atttaatgcc cttctaaaaa ctttagaaga
     5041 gcctccacag catgttatct ttatcttagt tacaacagat gttcataaac ttcctgctac
     5101 aatactttca aggtgtcaaa ggtatgattt tagacttgga actgaagaag agatctcaaa
     5161 aacattaaag aaaattctta agaaagaaga tctaaaaatt gatgaaaaag gattagaact
     5221 tgttgttagg aatgcaagag gaagctttag agatgcactc tctcttttag atgttgttat
     5281 aaacagtcag cttagaagtg aaaatcctaa ggagataaca gaagatgaag taagagctgt
     5341 gcttggtata ccagaagttt acatggttaa tgattttctt acctctttag ttaagggtga
     5401 tgggaaaaaa gcactggagt gtattgatga agtag
//
LOCUS       DAOSAB010000013         5373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_10430_length_5373_cov_3.663971, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000013 DAOSAB010000000
VERSION     DAOSAB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10430_length_5373_cov_3.663971"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3394)
                     /locus_tag="PK432_00595"
     CDS             complement(<1..3394)
                     /locus_tag="PK432_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59458.1"
                     /translation="MPQPDFTQDMLSVINNGLQNVDNIAAESSKKYAGATLVDPDMIN
                     QYRYQRDFDPMTFNPFDESNYDRWVAKETWATALRKGFDGFATRFGNTFVDYWKDYGR
                     MVKALINWDMDKMLPSESEMYAQYYKDQQNMFKNKVFVPYGEEDGIFNKSGISEFISN
                     AGFMMGTYSAFAVELLVDAAITAATVGFGAETFGVTGAKMAAKTGAKQVAKQGVKHLD
                     DFFKGIFKVGHMDDFAKITTKSASAVDNMAKAGKNLTNMTNASKAVISDGFDILTYNV
                     KNILKSKSAGEFFLNAAKGVPVIGSGVRQAERIVKAGKAGYGIATMAGMGIRGVQRMA
                     NEFNMSSTEAVFEAISTYGETLDFMLKDYHNRHGEGPNELDFSLMQDNALKAATANYQ
                     TNLAILLATNKIQFGVLFNKFPMHKSWLTRKIADEGVEGIVHVTTKGVGKVYDTKSIF
                     GGIKTVAKIGKEFGWKKASWEAAKLFRKDFLKFEVIEGIQEIIQESSNIAWKNYYAAQ
                     YAGAEKTITEAFGEGLGEQWSKQGLKVFIHGAMTGGIVRIPTRIIEATLEQSQKYVND
                     RLYGKENNPIRQAKAQRAKDIETVNKAMWAADYNKSSVSQLINTLKSSIQMDEAVKAN
                     NEFEWNNAKDNMMIQAAIAANRLGIDAAYTRAIEHMADDMTVDEFDKVMGIKLEDTKY
                     KTPQEYAKSIAKQVRDYSDTVTKIRKEFNNLVDPMAFAPESMNHYAAMITRAKQEEAI
                     EMIAMMRIKADRNIDRIKTLKEEIAKLPGLESSSQYAFNVLTSLEGSKSELGNIQSRI
                     LTIKESLKLQTLTASEKAKYEEQLKDLQEREEDIKTWQSFFIKNDKGQEVFIGETITE
                     EETGKVLQHDTESKKVKDLFRKIINSKNKEEGLPEISEKAFQESSIKLSDIIKLTNQT
                     KSLMDSYNSLYNAEKFQEVVKRMHEGHVKFIVSNNLEYYIQVMDRNILRYLAKKYNIS
                     KSELAKERELIAGTNYNLNPNNRMNYVFQLDTIYSIMDKNSKMKEDYKKFREQLNLQL
                     ENNKAYQELLSLVVDPSKFEEDIEKIESLMKQVGDVIIAFDVKVLSETDPTFAEQDIS
                     PDITSDTITSDDIIRIAAKLNKDMKLAPNEAKLYEDKKVKELV"
     gene            complement(3398..>5373)
                     /locus_tag="PK432_00600"
     CDS             complement(3398..>5373)
                     /locus_tag="PK432_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59459.1"
                     /translation="FAVIGRVQTETAIRNTMDTYGVDRKTAQIYVAEQLAPILADKEA
                     QKELAYNEAYRNISNKLNIFKQDLIKAGINGVPASKYPEYQALQQELATLEKIAENAA
                     LEKIKLQDPSYILSNMSYYFANNAKDQHIQEWASSTAMATMEVSIKDDNVWLTKYKES
                     QANWRHDQKLKFDMYKFNTEMQYKMDTEGVKQNADGTYTKTGTNSVTEVGHYTSDKTL
                     FKVDVLEQTRVDAENDLYNTAINSDDGLLNMIVRLNSRDGELSLDVFTNYQQTLNSLK
                     EYANRGGTVPYLNKKNLGKIKQMIIDLDLDAEAPVFIPNSAAAAQYLNYLSVAIYEKA
                     SEIIERNPNAVNVNSDLYKGLVATREAIENNLQVLNDIYDFWDNVGPEIMNSRMFYVN
                     PMNTVGFTPNDNPIMNPAKLTDAQKSILDKFAPDNIMSGVPVTKIYNIVVDNPNSISR
                     LLNQDIIKPNTFKGTGCYKNKDIEKIIEMFQKNPEIISKEFGNSIDVAYDPVDKSYIF
                     SLKPGESKDKKSGSITFKVDESAIESISVLRDTYSHVKNTSQLPSTMLGGEWGKLLND
                     KYYIIKSDPAISALGFKYMIKSNDYGGYTMEYAFQNPDGSWTKGQVSKNESTPQESLV
                     ALKALVSQLEAYYLASKKAALNNQYQSEKIIPF"
BASE COUNT         1638 a          944 c          605 g         2186 t
ORIGIN      
        1 ctactaattc tttaactttt ttatcttcat ataatttagc ttcattagga gctaatttca
       61 tatccttatt taacttagca gctattctta taatatcatc tgaagtaatg gtgtctgaag
      121 ttatatcagg agatatatct tgttcagcaa atgtaggatc tgtttcagat aatactttaa
      181 catcaaatgc tattataaca tctcctactt gtttcattaa actttctatc ttctcaatat
      241 cttcttcaaa tttagaagga tctactacta aagataatag ttcttgatat gctttattat
      301 tttctaattg taaattgagc tgttctctaa actttttata atcttctttc attttagaat
      361 tcttatccat aatactataa atagtatcca actggaatac ataattcatt cgattatttg
      421 gattaagatt ataattagtt cccgcaatta gctctctttc tttagctaat tctgattttg
      481 atatgttata tttctttgct aaatatctta aaatattcct atccattact tgaatataat
      541 attctaagtt attacttact ataaatttaa catgtccttc gtgcattctc ttaactactt
      601 cttgaaactt ttcagcatta taaagagaat tataactatc cattaaagat ttagtctgat
      661 tggttaattt aataatatca gataatttta tacttgattc ttgaaatgct ttttctgata
      721 tttcaggtaa accttcttct ttatttttac tattaattat ttttctaaat aaatctttta
      781 cttttttact ttctgtatca tgttgtaata ccttaccagt ttcttcttct gtaatagttt
      841 cacctataaa aacttcttga cctttatcat ttttaataaa aaaactttgc caagttttaa
      901 tatcttcttc tctttcttgt aaatctttta attgttcttc atatttagct ttctcagaag
      961 ctgttaaagt ttgtaatttt aaagattctt ttattgtgag tattctgctt tgtatatttc
     1021 ctaattctga tttagagcct tctaatgaag ttaaaacatt aaaagcatat tgagaagatg
     1081 attctaatcc tggaagtttt gctatttctt cttttaaagt tttaattcta tcaatatttc
     1141 tatcagcttt aattctcatc attgctatca tttcaatagc ttcttcttgt tttgctcttg
     1201 taatcatagc tgcataatga ttcatgcttt ctggagcaaa tgccattggg tcaactaagt
     1261 tattaaattc ctttctaatt tttgttactg tatctgaata gtctcttact tgtttagcaa
     1321 ttgatttagc atattcttgt ggagttttat atttagtatc ttctagttta atacccatta
     1381 ctttatcaaa ctcatcaaca gtcatatcgt ctgccatgtg ttctatagct ctcgtataag
     1441 ctgcatctat tcctaatcta ttagcagcaa tagcagcttg tatcatcatg ttatctttag
     1501 cattattcca ttcaaattca ttattagctt ttacagcttc atccatctga atagaacttt
     1561 ttaaagtatt tatcaattgt gatacagatg atttattata atcagcagcc cacatagcct
     1621 tatttacagt ctcaatatct tttgctcttt gagctttagc ttgtcttatt ggattatttt
     1681 cttttccata tagtctatca ttaacatatt tttgagattg ttctaatgtg gcttctataa
     1741 ttctagtagg tatcctaact ataccaccag tcatagcacc atgaataaat acttttaagc
     1801 cttgtttaga ccattgttcc cccaagcctt ctccaaatgc ttctgttata gttttttcag
     1861 ctcctgcata ttgagcagca taataatttt tccaagctat attagaactt tcttgtataa
     1921 tttcttgtat tccttctatt acttcaaatt ttaaaaaatc ttttctaaat aatttagctg
     1981 cttcccatga tgcttttttc caaccaaact cttttcctat tttagcaact gttttaatac
     2041 caccaaatat agatttagta tcatatactt taccaactcc tttagttgta acatgtacaa
     2101 ttccttctac accttcatct gctatctttc tagtaagcca tgatttatgc attggaaatt
     2161 tattaaataa aaccccaaat tgtattttat tagttgctaa taatatagcc aagttagttt
     2221 gataattagc tgtagcagct tttaaagcat tatcttgcat taatgaaaag tctaattcat
     2281 ttggaccttc tccatgacga ttgtgataat ctttaagcat gaaatctaat gtttcaccat
     2341 aagtactaat cgcttcaaaa acagcttctg tagatgacat gttaaattca ttagccattc
     2401 tttgaacacc tctaataccc ataccagcca ttgtagcaat accataacca gctttacctg
     2461 ctttaacaat tctttcagct tgtcttacac cagaaccaat tacaggtact cctttagcag
     2521 catttaaaaa gaactcacct gctgacttag attttaatat attctttaca ttataagtca
     2581 gtatatcaaa accatcacta attacagctt tactagcatt ggtcatgtta gtaagatttt
     2641 taccagcttt tgccatatta tcaactgcac tagcagattt agttgtaatt ttagcaaaat
     2701 catccatgtg acctacttta aaaatacctt taaagaaatc atctaaatgt tttactcctt
     2761 gttttgctac ttgttttgct cctgttttag ctgccatttt agcacctgta acaccaaatg
     2821 tttcagcacc aaatccaaca gtagcagcag ttatagcagc atctactaat aattctacag
     2881 caaatgcact atatgtaccc atcataaaac cagcattaga tataaattcg gatataccag
     2941 atttattaaa aataccatct tcttcaccat aggggacaaa gactttattt ttaaacatat
     3001 tttgttggtc tttataatat tgagcataca tctctgattc agaaggtaac attttatcca
     3061 tatcccaatt aatcaaagct tttaccattc ttccatagtc tttccaataa tcaacaaagg
     3121 tattaccaaa cctagtagca aatccatcaa atccttttct taaagcggta gcccaagttt
     3181 cttttgcaac ccatctatca taattacttt catcaaatgg attaaaagtc ataggatcaa
     3241 aatccctttg atatctatat tgattaatca tgtcaggatc taccaaagta gctcctgcat
     3301 attttttaga agattctgct gctatattat ctacattctg taatccatta ttaattacag
     3361 atagcatatc ttgtgtgaaa tctggttgag gcatatctta aaaaggtatt attttttctg
     3421 attgatattg attgtttagt gctgcttttt tactagcaag ataataagct tccaattgac
     3481 taactagagc ctttaaagct actaagcttt cttgaggagt gctctcattc ttagatactt
     3541 gtccttttgt ccaactacca tcaggatttt gaaaagcata ttccattgta tatccaccat
     3601 aatcattaga tttgatcata tatttaaaac ctagtgctga tatagcagga tcacttttaa
     3661 taatataata tttatcatta agtagttttc cccattctcc tcctaacatt gtagaaggca
     3721 attgagatgt atttttaaca tgactataag tgtctcttaa aacacttatt gattctattg
     3781 cagattcatc tactttaaat gtaatacttc ctgatttctt atctttagat tcaccaggtt
     3841 ttaatgaaaa tatataagat ttatctacag gatcataagc tacatctata ctattaccaa
     3901 attctttaga aattatttca ggattttttt gaaacatttc tataatcttt tcaatatctt
     3961 tattcttata acaaccagta cctttaaatg tatttggttt tataatatct tgatttaata
     4021 acctacttat actattagga ttatccacta caatattgta aattttagta acaggtactc
     4081 cagacataat attatcaggt gcaaatttat ctaaaatact cttttgagca tcagttaatt
     4141 tagctggatt cattattgga ttgtcatttg gggtaaatcc aactgtattc attggattca
     4201 cataaaacat tctactattc attatttcag gaccaacgtt atcccaaaaa tcgtatatat
     4261 catttaatac ttgtaaatta ttctctatag cttctcttgt agctactagt cctttatata
     4321 ggtctgaatt aacatttaca gcatttggat ttctttctat tatttcagaa gctttttcat
     4381 aaatagcaac acttaaatag tttaaatatt gagctgctgc agcactattt ggtataaata
     4441 ctggagcttc tgcatccaaa tctaaatcta taatcatttg ttttatttta ccaagattct
     4501 ttttattcaa ataaggtact gtccctcctc tattagcata ttcttttaaa ctattaagag
     4561 tttgttgata attagtaaat acatctaatg ataactcacc atctctacta tttaatctaa
     4621 caatcatatt taataaacca tcatcagaat taattgcggt attgtataaa tcattttctg
     4681 catcaactct tgtttgttct aacacatcaa ccttgaataa agttttatct gaagtataat
     4741 gcccaacctc tgtaacacta ttagttcctg tttttgtata agtaccatct gcattttgtt
     4801 taaccccttc tgtatccatc ttatactgca tttcagtatt aaacttatac atatcaaatt
     4861 ttaatttttg atcatgtctc caattagctt gtgattcttt atatttggtt aaccaaacat
     4921 tatcatcttt tattgatact tccatagttg ccatagctgt agaagaagcc cattcttgta
     4981 tatgttgatc tttagcatta ttagcaaaat aataagacat gttagataat atataagaag
     5041 gatcttgtaa tttaattttt tctaaagcag cattttctgc aattttttct aatgttgcta
     5101 attcttgttg caatgcttga tattctggat atttactagc aggtacacca tttataccag
     5161 ctttaattaa atcttgttta aatatattta atttattaga tatatttcta taagcttcat
     5221 tataagctaa ttctttttgt gcttctttat ctgcaaggat tggtgctaat tgttctgcta
     5281 cataaatttg tgctgttttt ctatctacac cataagtatc catagtattt ctaatagcag
     5341 tttcagtttg tactctacct attacagcaa att
//
LOCUS       DAOSAB010000014         5182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_10938_length_5182_cov_3.356739, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000014 DAOSAB010000000
VERSION     DAOSAB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10938_length_5182_cov_3.356739"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PK432_00605"
     CDS             complement(<1..503)
                     /locus_tag="PK432_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59460.1"
                     /translation="MKKVIEKIKPILLLLAMSLICVLMVFVVNQFKKEPEINEVTAQE
                     VSTSSMAESTSIDTSKPEEVKEKPVQESNEVLGDTFSCGGNAVHIASESTCIRNGSGS
                     VDYKTGSTRTGTLVKKDAKITLVSVNVPLELFSGMEVADSNRKITTETPTYKAAGEQI
                     MRKKFYRL"
     gene            complement(507..2117)
                     /locus_tag="PK432_00610"
     CDS             complement(507..2117)
                     /locus_tag="PK432_00610"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOK59461.1"
                     /translation="MNLTPHQQRQLKYVLILIIGVPLTVFAVYKGVQLISRAGEDPTP
                     HDIVVSNVTSSSLTISWITDKSVNGYVIPVLNGTEQNPMFDKRGEGKRKTHYVEVKGL
                     EPSTKYSFIIVSDEEKYTKGSSGPYEFTTASVMEDTPVPSYALGTVAGSDIKDTVVYI
                     TLEDKSSYPVSVDLPQSGNWTLELSLLRSIKDGTPLSVTDNTNLVVIARNGVDEGAVL
                     KGSYSTLFDKSGQLKNDLIIETVDKAEIISYFPREVLLGETIYAEPIPPEPEPEPEPE
                     PEPTNPIENPLEEVYIVRNDVPWNDLSTTESGSLQLTTGEDSVVIANLTDTSFVVVWR
                     SSEKEEGYVRYGTSKTKLDKEMIDSRDTLTNKGKYYSHYISSDRLSPNTTYYFEVHSG
                     GKIYDKGGSKYSVTTLPTLSTAPAFDMRTGKVLNAPDPSDWVVIFKIIDNDSKGTSGS
                     SGYLATLPDAQGSWTLVIGDARSSDGSSYFLFSDDDILQGSFLGAMDKKFDFNIAQDE
                     IQLDVNTLSGTTGSKVDLLPNYGIVGLE"
     gene            complement(2149..2448)
                     /locus_tag="PK432_00615"
     CDS             complement(2149..2448)
                     /locus_tag="PK432_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59462.1"
                     /translation="MEEIPAIFWMIVVGILVVFVCYVLYQLAMLLKESRDSIKGVNKA
                     VEDINNIVSEISGVLNIVKSPVLQISGALHGISTVASVISGIVDGFKSGEEDRND"
     gene            2532..3260
                     /locus_tag="PK432_00620"
     CDS             2532..3260
                     /locus_tag="PK432_00620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOK59463.1"
                     /translation="MNRFFIKHKLSIGDVIHLSDSDSEFVINNLKLKPENFIEIETYE
                     SIFLGVITDTSNNSVEVEIIEKIEDKKSKITFDVTVVQSLIGRERMNLVIEKAVEIGI
                     DRIIPVQSQYSNIKKNKAIKEYGLWGKIVKDAVEQSRNIKPTVIEKPIRLEDLKIGDN
                     VNKMCLSTENTDSISLRKYFESIDIKSPILFAVGPEKGWGSRDIEFFKENNFKFVKLE
                     GNILRSESTGLVVGSIIEYLKGNI"
     gene            3260..4282
                     /locus_tag="PK432_00625"
     CDS             3260..4282
                     /locus_tag="PK432_00625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOK59464.1"
                     /translation="MRKKVRSSYKKSRKKDQRRSPKYYTKEDIFISRMASILMVPRHR
                     IISIFSQRAKTTIRLNGLKGTPKETYKLLKARGYILTPIPWANNAYFVENRDKAEISQ
                     IEEYRNGLFYIQDLSSILATLILNPQEKENILDMCAAPGSKTTHIADITNNKATILAN
                     DSEVSRTNSLRNVVEQFGANNVRITLSDGREFGRKYPEYFDRVLLDAPCSGEGRIYMN
                     SSNPLRFWSIKKVKSSTYIQKELIDSAFRTLKTNGTLVYSTCTLEPDENEGVITYLLE
                     EFKNAKLEDIDIVNSKEFEDYKKHITSGITHWSGNQYNTSVKKCIRVIPSSEMMGFFI
                     AKIKKV"
     gene            complement(4284..>5182)
                     /locus_tag="PK432_00630"
     CDS             complement(4284..>5182)
                     /locus_tag="PK432_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOK59465.1"
                     /translation="GMLAEKALIPVVPSVDEFAYTILVMSEVQGENGSSSMASACGST
                     LALMSAGVPIRKMVGGIACGLVTNEDGSFHVLVDMRDVEDFYGDMDFKITGTADGVTA
                     VQMDTKMRGLSMDIVKETFKKSKEARLKILEVMKKAISEPKAEISKYAPKVARASIPV
                     DKIGELIGPGGKNIRELTEKTGAEVSVEEDGSVNIYSMNQKSIDEALSYVKKLGLVPT
                     VGEIYDGKVVGLMEYGAFVEIAPGLSGLVHVSEITDEYVKDVKKYLKEGDEVKVKLIS
                     KERDGKLRLSIKQAKEGSSEEK"
BASE COUNT         1751 a          945 c          788 g         1698 t
ORIGIN      
        1 aggcgataaa acttctttct catgatctgc tctcctgctg ctttgtatgt tggtgtttct
       61 gtagtaattt ttctgtttga atctgcaacc tccataccag aaaaaagctc aagaggcaca
      121 ttaacagaaa caagcgttat tttagcatct tttttaacca atgtccctgt ccttgttgat
      181 cctgttttgt aatcaacaga ccctgagcca ttcctaatac atgttgactc tgatgcaata
      241 tgcacagcat ttccaccaca tgagaatgta tcaccaagca cttcgttact ctcctgtact
      301 ggcttctctt tcacttcttc aggctttgat gtgtctatac ttgtactttc agccatagaa
      361 ctcgttgata cttcctgtgc agttacttca tttatctctg gctctttttt gaactgatta
      421 acaacaaaca ccattaatac acatataagt gacatagcta gaagcaaaag aatcggtttt
      481 atcttttcaa taaccttttt catcttttat tctaatccta ctataccata attaggaagt
      541 aaatctactt tagaaccagt agtcccacta agagtattaa catctaattg tatttcatct
      601 tgtgcaatat taaaatcaaa ctttttatcc attgctccaa gaaaactacc ctgtaatatg
      661 tcatcatctg aaaagaggaa gtaagaagaa ccatctgaac ttcttgcatc tccaattacc
      721 aatgtccaag aaccctgggc atctgggagg gtagcaagat accccgaact tcctgatgtt
      781 cctttactat cattatcaat tattttaaat ataacaaccc aatcagaagg atctggagca
      841 tttaaaactt tgcccgttct catatcaaat gcaggagcgg tagataatgt aggtaatgtt
      901 gtaacagagt acttactccc acctttatca tatattttac ctccagaatg tacttcaaaa
      961 taatatgttg tattaggaga caacctatca ctactaatat aatgagaata atatttacct
     1021 ttattagtca gagtatctct tgaatcaatc atctctttat ctaattttgt tttacttgta
     1081 ccatatctta catatccttc ttccttctca cttgatctcc atacaacaac aaagctggta
     1141 tctgtaagat tagcaattac aacactatcc tctcctgttg taagttgaag agatccactc
     1201 tcagtcgtag acaaatcatt ccaagggaca tcatttctta caatataaac ctcctctaaa
     1261 ggattctcta tgggattggt aggttctggt tctggttctg gctctggttc tggctctgga
     1321 ggaattggtt ctgcataaat tgtttcccct agtaaaactt ctctaggaaa atatgaaata
     1381 atttccgctt tatctactgt ttcaattatt aaatcatttt taagttgtcc acttttatca
     1441 aataaagtag aatagctacc ctttaatact gcaccttcgt ctacaccatt tcttgctata
     1501 acaacaagat tcgtattatc tgttactgat aaaggggtgc catcctttat acttcttaaa
     1561 agagaaagtt caagagtcca gttaccactc tggggaagat ctacagatac aggatagcta
     1621 cttttatctt ctagtgtaat atatacaact gtatctttaa tatcacttcc agctacagta
     1681 cctaatgcat atgagggaac aggtgtatct tccattacag aagctgttgt aaattcataa
     1741 gggcctgagc ttcccttagt atatttctcc tcatctgaaa ctataataaa agagtacttc
     1801 gtagaaggtt ctaagccctt tacctcaaca taatgagttt ttctcttccc ctcacctctt
     1861 ttatcaaaca tgggattttg ctctgtaccg ttgagcacag gaataacata gccatttacg
     1921 gatttatctg taatccagct gatagttagt gagcttgatg taacatttga aacaactata
     1981 tcatgaggtg taggatcctc tcctgctctt gatattaact gtaccccttt atacacagca
     2041 aaaactgtca aaggtacacc tattattaaa atcaacacat acttaagctg tctctgctga
     2101 tgaggagtca aattcatttc ataataatac taagaaatga ctatgatatc aatcatttct
     2161 atcttcttcc ccagatttaa atccatctac aattccagat attacacttg caactgtgga
     2221 aatcccatgt aatgcacctg agatttgtaa tacaggactc ttaacaatat taagaacccc
     2281 actaatctca gaaacaatat tattaatatc ctctacggct ttgtttaccc cttttataga
     2341 atctcttgac tctttaagaa gcattgctaa ctgatacaaa acataacata caaaaactac
     2401 taaaatccca acaacaatca tccaaaatat agcaggtatt tcttccatct ttttatctct
     2461 taaatttaaa atatattaca attatcgtta tttttaatgg tatatgtcaa gttaaatgat
     2521 ataattgcag aatgaataga ttctttataa agcataaatt aagtatagga gatgtaatac
     2581 atctctctga ttcagatagt gaatttgtta taaacaacct taaactaaaa ccagaaaatt
     2641 tcatagaaat agaaacatat gaatcaatat ttctgggggt aataactgat acatccaata
     2701 actctgttga ggttgaaata attgaaaaga ttgaagataa aaaaagtaaa attacatttg
     2761 atgtaacagt tgttcaatcg ttgataggaa gagaaagaat gaatcttgtt atcgagaaag
     2821 ctgtagagat aggaatagac agaataattc ctgtacaatc tcaatattcc aatattaaaa
     2881 agaacaaagc tattaaagag tatgggttat gggggaaaat agttaaagat gctgttgaac
     2941 agtcaagaaa tattaaacct acggtaattg aaaaacctat tagattagaa gatcttaaga
     3001 ttggtgataa tgtaaataag atgtgtctat caacagagaa tactgatagt ataagtttaa
     3061 gaaaatattt tgaaagtata gatataaaaa gccctattct gtttgcagta ggacctgaaa
     3121 aaggctgggg aagcagggat atcgagtttt tcaaagagaa taattttaag tttgttaagc
     3181 ttgaaggaaa tatactaagg tctgaaagta ctggcttagt tgttggaagt ataattgagt
     3241 atttgaaggg aaatatataa tgagaaaaaa agtaagaagc agttacaaaa aatctaggaa
     3301 aaaagatcaa agaaggtctc ctaaatatta cacaaaagag gatattttca tctctagaat
     3361 ggcaagtatt ctgatggttc caagacacag aataattagt atatttagtc aaagagcaaa
     3421 aacaacaatt cgtttaaatg ggttaaaggg tactccaaag gaaacatata aactgcttaa
     3481 agctagggga tatatactta ctccaatacc ttgggctaac aatgcatatt ttgttgagaa
     3541 tagggataaa gcagaaatat cacagattga agaatacaga aatggtcttt tctacattca
     3601 ggatttatca agtatacttg caactctaat attaaatcct caagaaaaag aaaatatctt
     3661 agatatgtgt gctgctcctg gtagtaaaac aacacatatt gcagatatta caaataataa
     3721 agcaacgata ttggctaatg attcagaggt tagtcgtact aactctctga gaaatgtagt
     3781 tgagcagttt ggtgcaaaca atgtaagaat aacattaagt gatgggagag agtttggaag
     3841 aaaatatcct gaatattttg atagggttct tcttgatgcc ccttgtagtg gtgagggtag
     3901 aatatatatg aacagctcta atccacttag attttggagt ataaaaaaag ttaaaagcag
     3961 tacatatatt caaaaggaat taatagattc tgcctttagg acattaaaaa ctaatggaac
     4021 acttgtatat tcaacatgta cacttgaacc tgatgaaaat gaaggagtta ttacatatct
     4081 gttagaagag tttaagaatg ctaagcttga agatattgat attgtaaatt caaaagagtt
     4141 tgaagattac aaaaagcata ttacaagtgg aattacacat tggagtggaa atcaatataa
     4201 tacatcagta aagaaatgta taagggtaat tccatcatct gaaatgatgg gctttttcat
     4261 tgcaaagatt aaaaaagtat aaactacttt tcctcgctac tcccttcctt tgcctgttta
     4321 atacttaacc tcagcttgcc gtccctttcc ttagagataa gctttacttt aacttcatct
     4381 ccttccttta aatatttctt tacatctttt acatattcat cagtaatttc tgagacatga
     4441 actaatcccg agagaccagg agctatttca acaaatgcac catactccat tagtcctaca
     4501 acttttccat catatatttc tccaacagta ggaacaagtc caagtttttt tacataactg
     4561 agtgcctcat caatagattt ctgattcata gaatatatat ttacagaccc atcttcttct
     4621 acactaacct ctgcacctgt tttctctgtt aactctctaa tatttttccc accaggacct
     4681 ataagttccc caattttatc tactggaata gaagctcttg caactttagg agcatatttt
     4741 gatatttctg cttttggttc agatatagct tttttcatta cctctagaat ctttaatctt
     4801 gcttctttag attttttaaa tgtctccttt actatatcca tacttaatcc tctcattttt
     4861 gtatccattt gtactgctgt aactccatct gctgtaccag taatcttgaa atccatatca
     4921 ccataaaagt cttcaacatc tctcatatct actaatacat ggaaactgcc atcttcatta
     4981 gttacaagac cacatgcaat acctccaacc atctttctaa taggaacacc agcagacatt
     5041 aatgcaagtg ttgaaccgca tgctgatgcc atagaagaag aaccgttttc tccctgaact
     5101 tctgacataa caagaattgt atatgcgaat tcatcaacag agggtactac tgggataaga
     5161 gctttttctg ctagcatacc at
//
LOCUS       DAOSAB010000015         4860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_11844_length_4860_cov_2.886576, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000015 DAOSAB010000000
VERSION     DAOSAB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11844_length_4860_cov_2.886576"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PK432_00635"
     CDS             <1..499
                     /locus_tag="PK432_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59466.1"
                     /translation="KKMPKRVIYTEHQNFLYNRALHGLSMYTPEEVIIMNPEKRKKIL
                     QIHKKTQKILNLWKQEIVNICANKLFTDIFPDMEITKCLVECYGIQGDPEYVNNMSFE
                     LLRISKQDIIAYLIEKKILPKNFNELNQLEDASRISNERDTNTSVNSRERLGRRTSEV
                     LSKTG"
     gene            757..1641
                     /locus_tag="PK432_00640"
     CDS             757..1641
                     /locus_tag="PK432_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD1 domain-containing DNA-binding protein"
                     /protein_id="HOK59467.1"
                     /translation="MLHYYVYTHKRPDTGQVFYVGIGRTPNPKVYSRAYTRARRSSEW
                     LSTVDSCEGKFIVDIVAIFDDHLSCCTEEMRLINSYGRKSLNIGYLVNKAPGGHKWKD
                     SIVVYQYALDGKFIAEWKDPKTAESVLGISHTSIYKSCRLFFKAGNYQFRTYKKEKIE
                     TYTDNKKKEIYQYSKLGSFIRSYASISEAAEHIKVKPERLSQTAKKNKSCKGYLWSFS
                     NEYCLVKRIIFQYATSGELVAQYASLPEAAKALKVNSVNAIDCAIKGKIQKQAYGFLW
                     TSQTNVKVFNNAAKTKNL"
     gene            1616..2038
                     /locus_tag="PK432_00645"
     CDS             1616..2038
                     /locus_tag="PK432_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59468.1"
                     /translation="MQQKLKICDNCKKPSIIWKSSGTGGKKYCKQCSSNTGVAKPSNT
                     KPTARKSTIPPHSQKRSKEERLYQAKRIIFLQEHPLCEAHLPGICTKHATDVHHSYSG
                     KDRQQHFLNESTWFATCRACHDWIHNHSKEARELGYLK"
     gene            2050..2400
                     /locus_tag="PK432_00650"
     CDS             2050..2400
                     /locus_tag="PK432_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59469.1"
                     /translation="MTYKKGMTKSKYKAKMRNFWDDRWYHNVSIETWCGLLFHLCGQV
                     WTSSQAFNQRRANELIFDAIEKLILEKKLTKDEAETLRNMLRSKDKENVHLAVMIIAK
                     IKPKVFAKNVDKIS"
     gene            2381..2635
                     /locus_tag="PK432_00655"
     CDS             2381..2635
                     /locus_tag="PK432_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59470.1"
                     /translation="MWTKYPDYKHAVKDWIAAINDKQSGAHAVYCLNRALRRAARDKV
                     ITREEQKSLWSMLKASDDDRYVALKALETKYNLTKTETNG"
     gene            2628..3401
                     /locus_tag="PK432_00660"
     CDS             2628..3401
                     /locus_tag="PK432_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59471.1"
                     /translation="MAKKKTPPAVLPTVTMQAEVSNNINLNLNQNDLIDLAIQEHLEK
                     LEKELEIVNAEITHNQNEIDKVESDEAEKLAAKVLKDDPDYIKFMKMLKVADIKITKE
                     GIDKRGYRKSDPYRLYATKDTNNFIESINQYPHENFTNLAHATRSIHKYNFYKTKYTS
                     LSINIEEHTAYITMIYIRHVNLTPDVAKSLEARIDILEERKRELLKKKFNLQKEFYEY
                     TFGEKKVKAKIVKASLQRSAEGQAILGMLQGATGVKLIG"
     gene            3426..3851
                     /locus_tag="PK432_00665"
     CDS             3426..3851
                     /locus_tag="PK432_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59472.1"
                     /translation="MAKSKRWSEKDIKQIKSIIEAAQVAGNTEHSGLAAAAKYFGVSL
                     NAVTIRWGRWKRGLQTGNLKKATTKVPMVRKKRKYVKRIKLNIPIWSDSSQPALLNVI
                     EKRKMHLELRKRVMELLDTAGPIKAVSIDLANKSFTVVY"
     gene            3856..4032
                     /locus_tag="PK432_00670"
     CDS             3856..4032
                     /locus_tag="PK432_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59473.1"
                     /translation="MAEEIPFRPEGYSKCYLALDALQLSNDITEEDYKLIRSMLDSED
                     EENLAMAELALWDK"
     gene            4062..>4860
                     /locus_tag="PK432_00675"
     CDS             4062..>4860
                     /locus_tag="PK432_00675"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOK59474.1"
                     /translation="MKTKDQIQEEALAALSKVRGGTAAMSVGTGKTLVGLKHMDLHYT
                     DTSFFLVVAPKKSIFQSWIDDAKKFGFDYLLDRITFTTYLSLPKQQLHYDAVYLDEAH
                     SLLESHAGWLNAYKGIKIGLTGTPPKFQHSLRGRLFNAHIPVVYNYITDDAVEDKILN
                     DYHIIVHKLKLNKAKILKAGKPPKTWMTSEQDSYNYWTNRINNAVGIKERQIMSILRM
                     KALMDFPTKEKYAKKLMDMMMYKTIIFANTQQQAEKFGVPTYHSKNLD"
BASE COUNT         1882 a          715 c          928 g         1335 t
ORIGIN      
        1 aaagaaaatg cccaaaaggg taatttacac agaacatcaa aactttttat acaatagagc
       61 tttacatggc ttgagtatgt atacaccaga ggaagtcatt ataatgaatc ctgagaaaag
      121 aaaaaagata ctacaaatac acaagaagac tcaaaagatt cttaacttgt ggaagcaaga
      181 aattgttaat atatgtgcta acaaactatt tacagatata tttcctgata tggagataac
      241 caaatgttta gtagaatgtt atggtataca gggtgatccc gaatatgtta ataatatgag
      301 cttcgaactt cttagaataa gtaagcaaga cattattgcc tatttaattg agaagaaaat
      361 attaccaaag aattttaatg aattaaatca gctagaagat gcaagtagaa tttctaatga
      421 acgagacact aacactagtg ttaactccag agaacgcctt ggaagaagaa cttctgaagt
      481 ccttagtaaa acaggataat gtcttgcaac aggcccgagg tggcattact gtttttaata
      541 aaacactggc taatgcccta attataggga aaagaagtgt cctagagccg gagaaagaag
      601 agagggggac aaagaatggt acagcctaag aaaaagatat gtgcaggttg tgggttggaa
      661 tcattcatct ggaagaatgt ggatgggaaa aagtacagat taggaaatat ctggaaaagt
      721 tagtatattt gtatactaat atacaaatat ttccatatgc tgcattatta tgtttatact
      781 cacaaaagac ctgatactgg tcaggtattt tatgtaggta taggtagaac acctaatcct
      841 aaagtttatt ccagagctta cacaagagca agaagatcat cagagtggtt atctacagtt
      901 gatagttgcg aggggaagtt tatagttgat atagtagcta tttttgatga tcacttgtct
      961 tgttgtactg aagaaatgag acttataaac tcatatggta gaaaaagtct aaacataggc
     1021 tatttggtaa ataaagcacc gggtggacat aaatggaaag attctatagt tgtttatcag
     1081 tatgctttag atggcaaatt tattgctgaa tggaaagatc ctaaaactgc agaatctgta
     1141 ttaggtatat ctcatactag tatctataag agttgtagat tattttttaa agctggcaac
     1201 tatcagttta gaacttataa aaaagaaaag atagaaactt atacagataa taagaaaaaa
     1261 gagatatatc aatatagtaa actaggctcc tttataagat cttatgcttc tatttccgaa
     1321 gctgctgagc atatcaaagt aaaacctgaa agactgagtc aaacagccaa aaaaaataaa
     1381 agctgtaaag gttacttatg gagtttttct aatgaatatt gtttagtaaa aagaatcatt
     1441 ttccagtatg caacttctgg agaactggta gctcaatatg catctttacc tgaagctgca
     1501 aaagctttaa aagtaaatag tgtaaatgca atagattgtg ctataaaggg taaaatacaa
     1561 aagcaagctt atggttttct atggactagt caaactaatg taaaagtatt taataatgca
     1621 gcaaaaacta aaaatttgtg ataattgtaa gaagccttcc ataatatgga aaagctctgg
     1681 gacaggtggg aaaaagtact gcaagcaatg ttcaagtaat actggagttg ccaaacctag
     1741 taatactaaa ccaactgcta ggaaaagtac aatcccacca cactcccaga aaagatctaa
     1801 ggaagaaaga ctataccaag caaagagaat tatctttttg caggagcatc ctttatgtga
     1861 ggctcatctt ccaggtatat gtactaaaca tgcaacagat gtacatcatt cttactctgg
     1921 taaggatagg caacagcatt ttcttaatga aagtacttgg tttgctacat gtagagcctg
     1981 tcatgattgg attcataatc atagtaaaga agcaagagaa ttaggatatt taaagtaaat
     2041 gatatatata tgacttacaa aaaaggaatg actaaaagta aatacaaagc taagatgaga
     2101 aacttttggg atgatagatg gtatcataat gtgtctattg agacttggtg tggcttactt
     2161 tttcatttat gcggtcaggt gtggacttct tcacaagcat ttaatcagag aagagctaat
     2221 gaactgatct ttgatgctat tgaaaaactt atcttagaaa agaaacttac taaagatgag
     2281 gctgaaaccc tcagaaatat gctccggtca aaagataagg agaatgtaca tctagctgtg
     2341 atgattatag ctaaaattaa accaaaagta tttgcaaaaa atgtggacaa aatatcctga
     2401 ttacaaacat gcagtaaaag actggattgc tgctattaat gataagcaat ccggtgcaca
     2461 tgctgtgtat tgtcttaaca gagctctaag aagagctgct cgggacaaag taataacccg
     2521 ggaagaacag aagtctttat ggagcatgct aaaagcatca gatgatgatc gttatgtagc
     2581 acttaaagca ttagaaacaa agtataattt aacaaaaaca gaaacaaatg gctaaaaaga
     2641 aaacaccacc tgcagtatta cctacagtaa caatgcaagc tgaagtaagc aacaacatta
     2701 acttaaacct taaccagaat gatttaattg atctggctat tcaagagcat ctagagaagc
     2761 ttgagaagga acttgaaatt gtaaatgccg agataactca taatcagaat gaaattgata
     2821 aagtagaatc agatgaagct gaaaaattag cagctaaagt tctcaaagat gatcctgatt
     2881 atataaaatt tatgaagatg cttaaagtag ctgatataaa aatcactaaa gaaggtattg
     2941 ataaaagggg ttatagaaag agtgatcctt ataggttata tgcaacaaaa gacacaaaca
     3001 atttcataga gtctattaat cagtatcctc atgaaaactt cactaatcta gctcatgcta
     3061 caagatctat tcataaatat aatttttaca aaacaaagta cactagtttg agtataaaca
     3121 ttgaggagca tacagcgtat attactatga tatatataag acacgttaat cttactcctg
     3181 atgttgcaaa gtctttagag gctaggattg acattcttga agaaagaaag agagaactct
     3241 taaagaagaa gtttaatcta cagaaagagt tctatgagta tactttcggt gagaagaaag
     3301 ttaaagctaa gattgttaaa gcatctcttc agagatcagc tgaaggtcaa gctattctag
     3361 gcatgttgca aggtgcaaca ggtgtaaaat taatcggtta atttaaaaac aaaaaacaaa
     3421 taacaatggc aaaatcaaaa agatggtctg aaaaagacat taaacaaatt aaatcaataa
     3481 ttgaagcggc acaggttgca ggtaatactg agcactcagg gttagcagct gcagctaaat
     3541 actttggagt atcgcttaat gcagtaacta taagatgggg gcgctggaaa agaggtctgc
     3601 aaactggaaa tcttaagaaa gctactacta aagttccaat ggtaagaaag aaaagaaagt
     3661 atgttaagcg cattaaacta aatataccaa tatggagtga ttcttctcaa ccagcattat
     3721 taaatgtgat agaaaaacgt aaaatgcatt tagaattacg caagagagtt atggagttac
     3781 tggatacagc aggaccaata aaagctgtat caatagatct tgctaacaaa tcttttactg
     3841 tagtttacta gttctatggc agaagagata ccattcagac ctgaaggata tagtaaatgc
     3901 tatttagcat tggatgctct gcagttgtca aatgatatca cagaagaaga ctataagcta
     3961 attcggtcta tgctagattc tgaagatgaa gagaatctgg ccatggctga attagcacta
     4021 tgggacaaat agaaacatat aacaattaaa tctaagaaat catgaaaaca aaggatcaaa
     4081 tacaagagga agctcttgcc gcattaagta aagtaagagg cggtactgca gcaatgtctg
     4141 taggaaccgg caaaacttta gtaggtctca agcatatgga tctacactat acagatacat
     4201 ccttcttctt agtagttgca ccaaagaaat ctatatttca gagttggatt gatgatgcta
     4261 agaagtttgg atttgattat ctgctagata ggataacatt tacaacctat ctttctctac
     4321 caaaacaaca attacactat gatgctgtat atttagatga agctcacagc ttattagaat
     4381 cccatgcagg atggttaaat gcatataaag gaatcaaaat aggattaaca ggaaccccac
     4441 caaaatttca gcactctcta aggggaagac tgtttaatgc acacattcca gtagtataca
     4501 actatattac ggatgatgct gtagaggaca agatcctaaa tgattatcac attatagtgc
     4561 ataaattaaa actaaacaaa gcaaagattc taaaggccgg gaaaccacct aagacatgga
     4621 tgacttctga gcaagactct tataattact ggactaaccg cataaacaat gctgtaggga
     4681 ttaaggaaag acaaatcatg agtatccttc gtatgaaagc tttaatggac ttccctacca
     4741 aggaaaagta tgctaaaaag ctaatggata tgatgatgta taaaactatt atttttgcca
     4801 atacacaaca gcaggctgaa aaatttggag tacctactta tcacagtaag aatcttgact
//
LOCUS       DAOSAB010000016         4808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_12009_length_4808_cov_3.671155, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000016 DAOSAB010000000
VERSION     DAOSAB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12009_length_4808_cov_3.671155"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PK432_00680"
     CDS             complement(<1..1261)
                     /locus_tag="PK432_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59475.1"
                     /translation="MSAVLNNGIFVRETNVKIGGHLDSYHLHNMLKGTSAHDLGVVDL
                     WVMAQKVEMPLYQMSNFGGKNIIEVDDPNGAYKWKTPVVSDLPYITEDVEPTRSNLGI
                     DGTTFKIKINKRAFGHGDIITYDKYNGLEMYITDDDILPSADGYVYTVQLVNNDNLAA
                     IDKNYLKPGTKIFRIGSVRGEYGEKFSDIGDVESGYREYYNFVGQAEAHSHFTVSSRA
                     DLILKKGMTADGLLVINELWRNFDKNLDPSITSLDDMVKKMGKDYVKKAVERGTLTRS
                     FITKLEAASLTKIANDIERYLMWGKGGRVKQDGPDDIRLSVGLWKQLDNSYKKIYTKA
                     GFSLDMFQIELHNFYNGKVEFKGPDPKREIIVQTGLAGMRLVNEAIKREALGTGLTVN
                     LDKSGINGISGSNAMDMHYGFAFTSYTI"
     gene            complement(1289..2425)
                     /locus_tag="PK432_00685"
     CDS             complement(1289..2425)
                     /locus_tag="PK432_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59476.1"
                     /translation="MENENVILDNIDDFLPIPGAESVVTSPNVLTTEKEETIDIDEDF
                     KDKLEDVIPSLNSEEEENEEKFKTSAKGKIKNNADVFKSLIDKGLLIPFDDDKPLEEY
                     TEKDWEELIKSNFEEKEKELQEKIQTEFFEALPKELQMATKYVLDGGTDLQGMFRALA
                     QTEQVKQLDAENPEHHEHIVRAYLYAKGFGTQEQIEEQIEEWSEMNMLGKKARQFKPA
                     LDEMHEEIVQQQVKKQEEYKKAMIAKREAYINNVADVLKTGELGGVKIDSKRQKMLFE
                     EMTTTKYQSMTGRPTNLLGKLLEDYQFGDKPRYDLIAEALWLLAEPDSYREAIMQVAR
                     NKTTDEITRKLKTEQDNKAATTNAPESKPEKRTIKRQSRNIFER"
     gene            complement(2536..3714)
                     /locus_tag="PK432_00690"
     CDS             complement(2536..3714)
                     /locus_tag="PK432_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59477.1"
                     /translation="MKPYQIGYNLVNNQIADMLIDELGTVILLDQNFLPQNSMDEDWG
                     RNNLAKAYVAMKNFQILPVDSTLANTESTVAMQHAQVLNLEQTNRLMTRVQLANFFKQ
                     QCFEAIGLSPQRMANIASHETATGIEQAINMSYTQTEMYFVNHAEHLMPRVHQMRTDL
                     AQYYHSNNPSVRLQYMTSMEERINFTINGNDLLSRELNVYITTKINQRMLLEQIKQLA
                     LTNNTTNATIYDLGNILKSDSITEVESVLKTIEEKTNERIRQEQQAAKELEQMKQQAE
                     ERKFMLKAEQEATQNALDRETNIKIAEIRAAGYGSMVDLNKNKQSDFIDAMEYLDKKE
                     AMQAKMDMERNKEERVTMIENKKLAMKEKELQVRENIANKQLQIARENKNKYDFKKTK
                     "
     gene            complement(3751..>4808)
                     /locus_tag="PK432_00695"
     CDS             complement(3751..>4808)
                     /locus_tag="PK432_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59478.1"
                     /translation="QYGQAKVQQQIAQLGLDLETEEGAQQAQQMMSPDVIKKLPEIES
                     FFKKDYRSMVEQWATHQHNVDEEKYSIKELENIAFRDMLITDSEFWHFKMNEDDYDIE
                     VWNPVLTFYYKAPETRYISDGSFVGRFDIMPIADVIDKYGYKMTEEQIASLEELYPIQ
                     NMQYILPGLDNDGAFYDATKSHEWNTEGPSLGMRQYTSFHDTFSDIDLIEDILSQNED
                     YSVINPNTKVRVTTAYWKSQRLIGHVTKVDETGFVTDLIVDENYKVTDKPIYDTSVMK
                     GKSKKNLVFGEHIEWIWINEVWGGVKIGPNKPLQHFDEKASGFTPIYLDVKPLKYQFK
                     GNTTLYGCKLPVEGCIF"
BASE COUNT         1403 a          856 c          569 g         1980 t
ORIGIN      
        1 gaatagtata acttgtgaaa gcaaatccat aatgcatatc cattgcattg ctaccactaa
       61 taccatttat acctgattta tcaagattta cagttaaccc tgtacccaaa gcttcacgtt
      121 taattgcttc attaacaagt ctcataccag ctaaaccagt ttgaactata atctcacgtt
      181 ttgggtctgg acctttaaat tctactttac cattatagaa attatgcaat tcaatttgga
      241 acatatcaag actaaatcca gctttagtat aaatcttttt ataagagtta tctaactgtt
      301 tccaaagacc tactgataat ctaatatcat ctggaccatc ttgtttaact ctaccacctt
      361 taccccacat caaatatctt tcaatatcat tagcaatttt agtaaggctt gcagcttcta
      421 atttagtaat gaatgatctg gtaagagttc ctctttctac tgccttcttc acataatctt
      481 tacccatttt cttaaccata tcatcaagag aggtaataga aggatctaaa ttcttatcaa
      541 agtttctcca caattcattt attactaaaa gaccatctgc tgtcatacct ttcttcaaaa
      601 tcaaatctgc tctactagaa acagtgaaat gtgaatgagc ttcagcctgt cctacaaagt
      661 tgtagtactc acgatatcca ctttctacat caccaatgtc tgagaatttc tcaccatatt
      721 ctcctcttac agaaccgatt ctgaagattt ttgtacctgg tttaagataa ttcttgtcta
      781 ttgcagcaag attgtcatta tttactaatt gaactgtgta aacataacca tcagcagaag
      841 gaagaatatc atcatctgtg atatacattt ccaacccatt gtatttgtca taagtgataa
      901 tatcaccatg tccaaatgca cgtttgttta ttttaatttt aaaggttgta ccatcaatac
      961 caagattaga acgagttggt tctacatctt ctgtgatata aggtaaatca gatactactg
     1021 gagtcttcca cttataagct ccatttggat catctacttc gatgatattc tttccaccaa
     1081 aatttgacat ttggtacaaa ggcatttcta ccttttgtgc cattacccaa aggtcaacaa
     1141 cacccaaatc atgagctgat gttcctttaa gcatgttatg aaggtggtaa gaatcaaggt
     1201 gtccaccaat ctttacgttg gtttctctta caaaaatacc attgtttaat actgcactca
     1261 ttttattaat tttaaattgt taatactatt atctttcaaa tatatttcta ctttgtcttt
     1321 taattgttct tttttctggt ttgctttcag gtgcattagt agtagcagct ttattatctt
     1381 gttctgtttt taatttccta gtaatttcat ctgttgtttt atttctagca acttgcatga
     1441 tagcttctct ataactatct ggttctgcta aaagccatag tgcttctgct attaaatcat
     1501 atctgggttt atctccaaat tgataatctt ctagtaattt accaagtaaa ttagtaggtc
     1561 ttccagtcat actctgatat ttagtagtag tcatttcttc aaataacatt ttttgacgtt
     1621 tgctatctat cttaactcca cctaattcac ctgtttttaa tacatctgct acattattaa
     1681 tgtaagcttc tcttttagca atcatagctt ttttatactc ttcttgtttt tttacttgtt
     1741 gctgtacaat ttcttcatgc atttcatcta gagcaggttt aaattgtctt gctttcttac
     1801 caagcatatt catttcagac cattcttcta tttgctcttc tatttgttct tgtgtgccaa
     1861 aacctttagc atataaataa gctcttacaa tatgctcatg atgttctgga ttttctgcat
     1921 ctaattgttt tacttgctct gtttgtgcta atgctctaaa cataccttgt aaatctgtac
     1981 caccatctaa tacatattta gttgccattt gtaattcttt aggtaaagct tcaaaaaatt
     2041 ctgtttgaat tttttcttgt agttcttttt ccttttcttc aaaattactt ttaattaatt
     2101 cttcccagtc tttttcagta tattcttcta aaggtttatc atcatcaaaa ggaattaata
     2161 atcctttatc aattaaagat ttaaaaacat cagcattgtt tttaattttt ccttttgcag
     2221 atgttttaaa tttttcttca ttttcttctt cttcagaatt aagtgaggga attacatctt
     2281 ctaatttatc tttaaaatct tcatcaatgt caattgtttc ttctttttcc gtagttaata
     2341 cattaggaga agttactact gattctgctc ctggtatagg gagaaaatca tcaatattat
     2401 ctaatattac attttcgttt tccatacttt ttatcttttt tggctactgt ttctattata
     2461 aagatataaa ataaatttta aacatttaca ttttttaaat ttaaaaatca actatagtca
     2521 aatacatagc taaagctatt ttgtcttttt gaaatcatat ttgtttttat tctctcttgc
     2581 tatttgtaac tgtttattag caatgttttc tcttacttgt aactcttttt ctttcattgc
     2641 taatttttta ttttctatca ttgttactct ttcttcttta tttctttcca tatccatttt
     2701 tgcttgcata gcttcttttt tatcaagata ttccatagca tcaataaaat cactttgttt
     2761 gtttttattt aaatctacca tagaaccata tcctgcagca cgtatttctg caatcttaat
     2821 attagtttct ctatccaaag cattttgtgt agcttcttgt tctgctttaa gcatgaattt
     2881 tctttcttct gcttgttgct tcatttgttc taattcttta gcagcttgct gttcttgtct
     2941 tattctttca ttagtttttt cttctatagt tttaagaact gattcaactt ctgtaataga
     3001 atctgatttt aaaatattac ctaaatcata aattgtagca ttagttgtat tattagttaa
     3061 tgcaagttgt tttatttgtt ctaataacat tctttgatta atcttagttg ttatataaac
     3121 attaagttct cttgataata aatcattacc attaattgta aaattaatcc tttcctccat
     3181 agaggtcatg tattgtaatc ttacactagg attattagaa tgataatatt gtgctaaatc
     3241 agttctcatt tgatgtactc taggcattaa atgttcagca tgatttacaa aatacatttc
     3301 tgtttgagta taactcatat taattgcttg ttctatacct gtagcagttt cgtgtgatgc
     3361 tatattagcc attctttgag gagataatcc tatagcttca aaacattgtt gtttaaagaa
     3421 attagctaat tgtactctag tcattaatct attagtttgt tctaaattta atacttgagc
     3481 atgttgcatt gctacagtac tttctgtatt ggctaatgta gaatctacag gtaatatttg
     3541 aaaattcttc attgctacat aagccttagc taaattattt ctaccccaat cttcatccat
     3601 tgaattttgt ggtaagaaat tttgatctaa taagataaca gttcctaatt catctatcaa
     3661 catatcagct atttgattat ttaccaaatt atatccgatt tgataaggtt tcattttatc
     3721 aactaatgaa gtactcttac tatttctttc ttaaaatata catccttcta ctggtaattt
     3781 acagccatat aaagtagtat tacctttaaa ttgatatttg agaggtttaa catctaaata
     3841 aataggagta aatcctgatg ctttttcatc aaaatgttgt aatggtttat taggtcctat
     3901 cttaactcca ccccaaactt cattaatcca aatccattct atatgttctc caaatactaa
     3961 attcttttta gatttacctt tcattacaga agtatcatat attggtttat ctgtaacttt
     4021 ataattttca tctacaatta aatctgttac aaaccctgtt tcatcaactt tagtaacatg
     4081 tccaattaat ctctgtgatt tccagtatgc tgttgttact cttacttttg tatttggatt
     4141 aataacagaa taatcttcat tttgacttag tatatcttct attaaatcta tatcagaaaa
     4201 agtatcatgg aaagaagtat attgtctcat tcctaaagaa ggaccttctg tattccattc
     4261 atgtgatttt gtagcatcat aaaaagcacc atcgttatct aaccctggca aaatatattg
     4321 catgttttgt attggatata attcttccaa agatgcaatt tgctcttctg tcattttata
     4381 tccatattta tctataacat ctgctatagg cataatatca aatctaccta caaaactacc
     4441 atctgatata tatcttgtct ctggagcttt gtaatagaat gtgagaacag gattccatac
     4501 ctctatgtca taatcatcct cattcatttt aaaatgccaa aattcagagt ctgttattaa
     4561 catgtctcta aaagcaatgt tttctaattc tttaatagaa tacttttctt catctacatt
     4621 atgttgatgt gtagcccatt gctctaccat agatctataa tcctttttga aaaaagattc
     4681 tatttcaggt aattttttaa ttacatctgg agacatcatt tgttgtgctt gttgagcacc
     4741 ttcctctgtt tccaaatcta atcctaattg tgcaatctgt tgttggactt ttgcttgccc
     4801 atattgca
//
LOCUS       DAOSAB010000017         4788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_12078_length_4788_cov_4.092119, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000017 DAOSAB010000000
VERSION     DAOSAB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12078_length_4788_cov_4.092119"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PK432_00700"
     CDS             <1..1070
                     /locus_tag="PK432_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK59479.1"
                     /translation="EIVELQNKLASSNAPIELKEQTMKTIQRLERMARLGNYSSEFES
                     VDKYVDWITRIPWGVVSKDNLDIENARRMMDSTHYGMDTIKEMILDYLAVMQLKNMQR
                     ARGGNVTDNSPQIPTLLFVGLQGVGKTSIAQSIAKAMGRKFARISLGAVGDVRTLRGV
                     PRHEPDAEPGQIIKSLVRAGTMNPVILIDEIEKSSQNKGVQNDMMAALLEILDPEQNS
                     TFVDHYIDYPVDLSQVFFICTANNLGGLSAALLDRLEVIRFTSYSDQEKEIIAKSYIL
                     PRTLASMGLPPEYIQISEDVWPLIIRPVGFDAGIRQLERNIATLLRSAAREIVSGKSI
                     PIVITPDNLKQYVLPDQGPLS"
     gene            complement(1067..1279)
                     /locus_tag="PK432_00705"
     CDS             complement(1067..1279)
                     /locus_tag="PK432_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59480.1"
                     /translation="MKMKNNQNNNNKTIYQYNVTANSDINYENIPKINNNGNGTPNPP
                     IQKQPFEPALKRAQMALKLVNPFKGV"
     gene            complement(1248..3653)
                     /locus_tag="PK432_00710"
     CDS             complement(1248..3653)
                     /locus_tag="PK432_00710"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK59481.1"
                     /translation="MAEDNKYQSKTIEELKKKESTLTENKFATFTQDLAKDLEQIQEK
                     KNNKDNQLNQKERYNDPVDEKKEEVKKNPILKFLDNFIPADQKTKSQTPTQSQPGESK
                     VSETKYEGLTLQDIIAPIDLEVDFNHLQMGNYYFRTFFISGYPRFVGPNWLSPIINFE
                     HSLRISTFYYPVDSKIIMEKLKRKIAEMEATIYTQMEERKVIDASLKVALSDAQQLQD
                     SIAEGTEKFFHFAMYITIYAPNKELLEKYSRNVVSTLAAIGVTAKPATLQQEPGFIST
                     QPLGLDKLYVTRNMDTTSLATTFPFVTSELTMDHGIMYGINMHNKSLVIFDRFELENA
                     NSVVFAKSGAGKSYFVKLEALRSLMLGTDIIIIDPEREYEDLSRAVGGSYISFSQDKG
                     DKMNPFELSGVYEEGEDELRIKIQSLIGFFKIMFDGLSNTEISILDRALILTYREKGI
                     TYDPATQKNPPPMLEDLYKVLKGMAEEEAHDMARRMERFIIGSGAGVFNEATNFEINN
                     SFTVFSVRDLQEELKPLSMYLMLDFIWTVIRKEKKRRLLIVDEAWYMMQRKDSAEFMY
                     SIAKRARKYYLGLTTITQDVADFMHNDLGKAIIQNASMSMLMMQSPSAVEAIQEVFHL
                     SDGEKAFLLNCEKGQGLFFAGQNHVAMQVISSVQENTLITSDPRELERMKLMGEREKS
                     IEELSAIFEPPATQMSVSTETPKNKDLIERAVGKRKEDIKEIKEEREKYQEELKKRLE
                     EQDKQLNPDKYESQKSMGQLIEENTVPGQVMKERKEIQKGKMHITPQGVISDENEEQP
                     KQQ"
     gene            complement(3655..4299)
                     /locus_tag="PK432_00715"
     CDS             complement(3655..4299)
                     /locus_tag="PK432_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59482.1"
                     /translation="MDANARARTTASTQDHLEIKDIVDDLVITKSGTVAMVIQTSAIN
                     FDLLAEYEQDSKIFAFAGLLNSLNFHIQILIRTRRIDISNYVNYLKNQEKPDMTPGLK
                     KQLNIYTQFIQRLIIENDVLDKNFFLVIPYNPGGSIPTANVLKSKKSKQEIEQNMQMK
                     QSQLLEKAKIFLFPKRDHILKQLNRMGLGGKQLTTKELITEFYTIYNPEEERGE"
     gene            complement(4299..>4788)
                     /locus_tag="PK432_00720"
     CDS             complement(4299..>4788)
                     /locus_tag="PK432_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59483.1"
                     /translation="TKPAIQPEKKKQKIFGGSKIDHTGQEVYTEGSEKEQPILQTVKQ
                     VTPSPLISTDEKIIINEENISKYQFKIQSVDKLPGNINLWLCTKDNQPIPNVQAYLKD
                     KDGKILYANKTGKNGYFLTNKEYPEGIYNIEFEGVQFTIPSISIVLTKNMGKLPYRIE
                     VK"
BASE COUNT         1342 a          802 c          789 g         1855 t
ORIGIN      
        1 ctgagatagt tgagctacag aataaattgg catcttctaa tgcccctatt gagctaaaag
       61 agcagacgat gaaaactatc caaaggcttg agagaatggc taggcttggt aattatagct
      121 ctgagtttga gagtgtagat aaatatgttg attggattac aagaatccca tggggagttg
      181 tatctaagga taatctggat atagaaaatg caagaagaat gatggattct actcattatg
      241 gtatggatac tataaaggag atgattcttg attatcttgc agtaatgcaa ttgaaaaata
      301 tgcaaagagc tagaggtgga aatgttacag ataattcacc acaaatacct actttacttt
      361 ttgtaggtct tcagggtgtt ggaaaaacat ctattgcaca atcaatagca aaagcaatgg
      421 gaagaaagtt tgcaagaata tctcttgggg cagtgggaga tgtaaggaca cttagaggtg
      481 ttcctagaca tgagcctgat gcggaacctg ggcagattat aaagtcttta gtaagagctg
      541 gtactatgaa tcctgttatt cttatagatg aaattgaaaa atcaagtcag aacaaaggtg
      601 ttcagaatga tatgatggct gcactgcttg agatacttga tcctgagcaa aactctacat
      661 ttgtagatca ttatattgac tatcctgtcg atttatctca agtattcttt atatgtactg
      721 caaataactt aggagggcta tctgctgcat tgcttgatag actcgaggtt attcgtttta
      781 caagttatag tgatcaagag aaagagatta ttgctaaatc atatatactt cctagaacat
      841 tagcatctat gggattgcct cctgaataca ttcaaatatc tgaagatgta tggcctttga
      901 ttataagacc agttggtttt gatgcaggta tacgtcagct tgaaaggaat attgcaacac
      961 tacttagaag tgcagcaagg gaaattgtga gtggaaagag tattcctatt gtaattacac
     1021 ctgataatct caaacagtat gttcttcctg atcaaggtcc tttaagttaa acacctttaa
     1081 aaggatttac tagttttaat gccatctgtg ctctctttag tgcaggttca aacggttgtt
     1141 tttgtattgg agggttgggg gtaccattcc cattattatt aatcttgggg atattctcat
     1201 agttaatgtc cgaattagca gttacattat attgatatat tgttttgtta ttgttgtttt
     1261 ggttgttctt cattttcatc agatattact ccttgtgggg taatatgcat tttacctttt
     1321 tgaatttctt ttctctcctt cattacttgt cctggaacag tattttcttc aattaattgt
     1381 cccatacttt tttgtgattc atatttatca gggtttagtt gtttatcttg ttcttctagt
     1441 cgctttttta gctcctcttg atatttctct ctttcttctt ttatttcttt aatatcttct
     1501 tttctctttc ctacagctct ttctattaaa tctttattct ttggtgtttc tgtagataca
     1561 gacatttgag ttgcaggagg ctcaaatatt gcagaaagct cttcgatgct tttctctctt
     1621 tcacccatta atttcattct ttcaagctct cttggatctg aggtgataag ggtgttctct
     1681 tgtacacttg atatgacctg cattgctaca tgattttgac ctgcaaagaa taatccttga
     1741 cctttttcac agtttagcaa gaaagctttt tctccatcag aaagatgaaa tacctcttga
     1801 attgcttcta cagcagaagg gctttgcatc atcagcatag acattgatgc attttgaatt
     1861 attgctttac ctaaatcatt atgcataaag tctgcaacat cttgtgttat tgttgtaagt
     1921 ccgaggtagt attttcttgc tctcttagcg atagagtaca taaactctgc ggaatctttc
     1981 ctttgcatca tataccatgc ttcatctact atcagtaatc ttctcttttt ttcttttctt
     2041 attacagtcc atatgaagtc gagcataaga tacattgata aaggttttag ttcttcctgt
     2101 aagtctctta cgctaaaaac tgtaaaagaa ttatttattt caaaattggt agcctcatta
     2161 aaaactccag caccacttcc gattataaat ctctccattc ttcttgccat gtcatgagct
     2221 tcttcttctg ccatcccctt taataccttg tataaatctt caagcatagg aggagggttc
     2281 ttttgtgttg caggatcata tgttatgcct ttttctctgt atgtaagaat taaagctcta
     2341 tcaagtatgg atatttcagt attacttagt ccatcaaaca ttatcttaaa gaatccaatg
     2401 agagattgta tctttattct taactcatcc tcaccttctt catatactcc tgaaagttca
     2461 aatggattca ttttatcccc tttatcctgt gagaaagata tatatgaacc tcctacagct
     2521 cttgaaagat cttcatactc tctttcaggg tctataatga taatatcagt tccaagcatt
     2581 aagcttctta atgcctcgag ttttacaaaa tagcttttac cagcaccaga tttcgcaaat
     2641 acaacagaat tagcattttc aagttcaaat ctatcaaaaa taactagact cttgttatgc
     2701 atatttatcc catacataat tccatgatcc attgtaagtt cagatgtgac aaatgggaat
     2761 gtagtagcaa gtgaggttgt atccatattt cttgttacat acagtttgtc taatcctagt
     2821 ggttgtgtac taataaagcc aggctcttgt tgaagtgttg caggttttgc agttactcct
     2881 attgcagcta aagtagatac tacattacgg ctgtattttt caagtaattc tttattaggt
     2941 gcatatattg ttatatacat agcaaagtgg aagaatttct cagttccttc tgcaattgaa
     3001 tcttgaagct gttgtgcatc agatagtgct acctttaagg aagcgtcaat aacttttctt
     3061 tcttccattt gcgtataaat tgttgcttcc atctcagcaa tcttcctttt taatttctcc
     3121 attattattt tagaatcaac agggtagtag aatgtactta ttcgcagaga gtgttcaaaa
     3181 ttaataattg gagaaagcca gtttggacca acaaatctag ggtagccact aataaagaaa
     3241 gttctgaagt agtaattgcc catctgtaaa tggttaaaat caacctcaag atcaattgga
     3301 gctattatgt cttgaagtgt taaaccttca tatttagttt cacttacttt actctctcct
     3361 ggttgtgatt gtgtaggtgt ctgagattta gttttctgat cagcagggat aaaattatct
     3421 aagaatttaa gtattggatt cttttttacc tcttctttct tttcatccac aggatcattg
     3481 tacctttctt tctggttcag ttgattatct ttattatttt ttttctcttg aatttgttct
     3541 aagtctttag ctaaatcctg agtaaacgta gcaaatttat tctcagtaag agtgctctct
     3601 ttctttttta attcttctat tgtttttgat tgatatttat tatcttctgc catattattc
     3661 tcctctttct tcttctggat tatatatagt gtaaaactct gtaattaatt cttttgttgt
     3721 aagttgtttt cctcccaaac ccattctatt taattgcttt aatatatgat ctcttttagg
     3781 aaagagaaat atttttgcct tttctagaag ctgactctgt ttcatttgca tattctgttc
     3841 aatttcctgt ttagactttt tggattttaa tacattagca gtaggtatac taccacctgg
     3901 gttataaggt ataactagga agaagttttt atcaaggaca tcattttcaa taattaacct
     3961 ctggataaat tgtgtatata tgtttaactg tttttttagt cctggtgtca tatcaggctt
     4021 ctcctggttt ttaagatagt ttacataatt agaaatatct attcttctag ttcttattaa
     4081 tatctgtata tggaagttta aagaattaag aagtcctgca aatgcaaata ttttactatc
     4141 ctgctcgtat tctgctaaaa gatcaaagtt aattgctgat gtttgaatta ccattgccac
     4201 agtaccactc ttagtaatta ctaaatcatc tacaatatcc tttatttcaa ggtgatcttg
     4261 agttgatgct gttgttcttg ctcttgcgtt tgcgtccatt attttacttc tattctataa
     4321 ggtaatttcc ccatattctt tgtaagtaca atacttatgg aaggtattgt aaattgtact
     4381 ccttcaaatt caatattata tataccttca ggatactcct tattagtaag aaagtatcca
     4441 ttctttcctg ttttatttgc ataaagtatc tttccatctt tatcttttaa atatgcctga
     4501 acattaggaa tcggctgatt gtcttttgtg cataaccata ggttaatatt tccaggtaat
     4561 ttgtctacac tttgaatttt aaattgatac ttagatatat tttcttcatt aattattatt
     4621 ttttcatcag tagaaattag tggagaagga gttacttgtt ttacagtctg tagtataggt
     4681 tgctctttct ctgaaccttc agtatatacc tcttgtcctg tatgatcaat tttagaacct
     4741 ccaaatattt tttgtttttt cttttcaggt tgaattgcag gtttagtt
//
LOCUS       DAOSAB010000018         4731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_12237_length_4731_cov_3.102866, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000018 DAOSAB010000000
VERSION     DAOSAB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12237_length_4731_cov_3.102866"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1408)
                     /locus_tag="PK432_00725"
     CDS             complement(119..1408)
                     /locus_tag="PK432_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOK59484.1"
                     /translation="MFEDIRKEFPILKDKFLGKGLVYLDSAATTQKPQEVIDRINQYY
                     EQENANIHRGPYDLSINATNLWQEAHETVSDFINSDSYKEIFFVRNSTEGLNFLVNTV
                     GRKLLSDGDIVVITEMEHHSNIIPWMLLQKEIKFRIEYIPVKDDYTLDLNWFKDLVKK
                     EGEKIKIVSVVHISNVLGVKNDVEEIFKIAKKVDAFTMLDAAQSIARVPVDVKSLGCD
                     ALVFSGHKVYGPTGSGVVYVGMRHLEELVPWMGGGEMVSLVTKKGFEYNELPWRFEAG
                     TPDIAGGIGLGAAIEWLKGRVDMVGGWDMIINHEQGLIGYFLNEFNGIDWFVHFGPDD
                     ISQKYGSIAFTIKGFRFRGCKDVKSIQTKKDGEGIVDLLNKKRICFREGFHCAQPLHD
                     RFKIGPTLRFSLGIYNNESDMKYAANAVKEAVLSGLK"
     gene            complement(1428..2015)
                     /gene="dcd"
                     /locus_tag="PK432_00730"
     CDS             complement(1428..2015)
                     /gene="dcd"
                     /locus_tag="PK432_00730"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOK59485.1"
                     /translation="MTEVKLRVLNMILSDKSIKEKINTGEISISPFSEDSLQPASYDL
                     HLDKNIMYFNTNGNNIIDVKKPVDELMIRTQISDSGFVIEPHQLVLANVIEITGVDSK
                     HVGRLEGKSSLARIGLIIHATAGFLDPGNKLRLTLEMVNLSPLPIRIYVGMKIAQIAF
                     EELDTPCEKPYGSKSLNSKYVGDLTVQASKIWKNF"
     gene            complement(2002..3597)
                     /locus_tag="PK432_00735"
     CDS             complement(2002..3597)
                     /locus_tag="PK432_00735"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOK59486.1"
                     /translation="MKGTFRKDIQIASIESEDDWNQFKVHTLLENVNQKQPSINAYLG
                     ARYSRSSDSVIDIASEIIKNNTDAAQRLEAIFHGYGHKSVGDMAQLFVCIENIPMFTA
                     MKIFYLNSVISGQERSTRYQNFEEPEYIRIPREICNDKKIRQEYEDIILKQMHDYSVL
                     LKKTKEIFKEYFNINEDIKEEKSALQARSFDCARYLLPYGVQTSMAAVMSARNWSELI
                     SYLNASNGIVDREVGKLIYSLLGDSDIEIAGYVREADGLIRHTEANYSRKNSTKEILE
                     YLKKKVSPQKSYISQGSESDSCTVSYNDNAVEDLFTHYELLINPLGGENELEFDDEDS
                     EYIGEVLFKNHNQHSLIGNLGQSGSIKIEGIASLCTLKDLNRHRSFERFIPLLHDEIN
                     IDNELNRDSKDCFFLCNYLDISEMSELKKEYEKALIGTYNMIKRWRNDSLKVVSEECS
                     QEFTRYLLPHAHATRYCFYASIDDLQYTVNLRSARGGHIAYRTLVYRWLELLNSNDSM
                     WYSLLKKIEMPDPASKAQFIDRS"
     gene            complement(3594..>4731)
                     /gene="glyA"
                     /locus_tag="PK432_00740"
     CDS             complement(3594..>4731)
                     /gene="glyA"
                     /locus_tag="PK432_00740"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOK59487.1"
                     /translation="YVSEDVLKAMGSILTNKYSEGYPHKRYYGGNEYIDKIEEECIKR
                     AKELFGAEHVNVQPYSGSPANLAVHFAFLKPGDRTMGMDLSAGGHLTHGFKVSISGKY
                     FDSVSYGVNKEGYIDYEEVRRIALEHEPKLIWSGFSAYSRIVDWKEFRKIADEIGAIL
                     VADIAHVAGLVAAGLYPSPVGIADVVTTTTHKTLRGPRGAIIMCKEEYGELIDKAVFP
                     GLQGGPHNHITAGIAVALKEAQSDEFKEYARQVISNAKALASSLANKGFSIVSGGTDN
                     HMFLVDTIKSIGKSGKEVSDILESVNITLNKNAIPFDERKPWDPSGVRIGSAAVTTRG
                     MKEKDMFQISEYIYRALYDSSEDNLKRIKDEVKDFSKKFNIPGI"
BASE COUNT         1491 a         1038 c          548 g         1654 t
ORIGIN      
        1 tttattctga agctcaacta tctcagacat tacattatca tttactgttg tgtaatccat
       61 atcaaaatat atcacatata ctatgaagat tcttgaccta taacatcaaa taatatactt
      121 acttcaaacc acttaaaaca gcctccttaa cagcattagc agcatacttc atatcagact
      181 cattattata tattcctaaa ctaaatctta atgtaggacc aatcttaaat ctatcatgca
      241 gtggttgtgc acaatggaaa ccctctctaa aacaaattct ctttttatta agtaaatcta
      301 caataccttc tccatctttc tttgtttgaa tactttttac atctttacac cctctaaatc
      361 tgaaaccttt tatagtaaat gcaatactcc catatttctg agaaatatca tcaggaccaa
      421 aatgaacaaa ccaatctatt ccattaaact catttaaaaa ataccctatt aacccctgtt
      481 catgattaat tatcatatcc caccccccaa ccatatctac acgaccctta agccactcaa
      541 ttgcagcacc caaaccaatt cctcctgcaa tatcaggagt accagcctca aatctccatg
      601 gcagttcatt gtattcaaaa ccctttttcg taactaaaga aaccatctct ccaccaccca
      661 tccatggaac aagctcttca agatgcctca tacccacata cacaacacca cttcctgtag
      721 gcccatacac cttatgtcca gaaaatacta atgcatcgca acccaaactt ttaacatcaa
      781 caggtactct agcaatgctt tgtgcagcat caagcattgt aaaagcatct accttcttag
      841 caatcttaaa tatctcctct acatcattct taacacccaa tacatttgaa atatgaacaa
      901 cagatacaat ctttatcttc tccccctcct ttttaactaa atccttaaac caatttaaat
      961 ctaatgtgta atcatctttt acaggtatat actctattct aaactttatc tctttttgca
     1021 acagcatcca tggaataata ttactgtgat gctccatctc tgtaattaca acaatatccc
     1081 catcagaaag aagctttctt cctacagtat taacaagaaa atttaatcct tcggtactgt
     1141 ttcttacaaa aaatatctct ttatatgaat ctgaatttat aaaatcagaa acagtttcgt
     1201 gagcctcttg ccaaagatta gtagcattaa tagaaaggtc atatggaccc ctatgaatat
     1261 tagcattctc ctgctcataa tactgattaa tcctatctat aacctcttga ggtttctgag
     1321 tggtagcagc actatcaaga tatacaagtc ccttacctaa gaatttatct tttagaatag
     1381 ggaactcttt tctaatatct tcaaacatat taattacatt gtttaattta aaaattcttc
     1441 catattttac tcgcttgtac tgtcaaatca ccaacatact tactatttaa acttttacta
     1501 ccatatggct tctcgcaagg agtatcaagc tcctcaaaag caatttgtgc aatcttcata
     1561 cctacatata ttctaatagg aagaggagaa agattaacca tttctagtgt taatcttaat
     1621 ttgtttcctg gatctaaaaa tccagcagta gcatgtataa ttaaccctat tcttgcaaga
     1681 gagctctttc cttcaagtct tccaacatgt ttactatcta ctcccgttat ctcaatgaca
     1741 ttagcaagta caagctggtg tggttctatt acaaatccgc tatcacttat ctgtgttcta
     1801 atcattaatt catcaacagg tttcttaaca tcaataatat tatttccatt agtattaaaa
     1861 tacataatat ttttatccaa atgtaaatca tatgatgcag gctgaagact atcctcagag
     1921 aaaggagaaa tagatatctc tcctgtgtta attttttcct taatgctttt atctgaaagt
     1981 atcatattta aaactcttaa tttaacttct gtcaataaat tgagccttag atgcaggatc
     2041 aggcatttca atcttcttaa gtaaagaata ccacatgcta tcattactat ttaataactc
     2101 taaccatcta tatactaatg ttctgtatgc aatatgacca cctcttgcac ttctaagatt
     2161 aactgtatac tgcaggtcat caatagaagc atagaaacag tatctagttg catgagcatg
     2221 aggaagaaga tatctagtaa actcctgaga gcactcctca gacacaactt taagggaatc
     2281 atttctccat ctctttatca tattatatgt ccctattaaa gctttctcat actctttctt
     2341 taattcggac atctcagata tatcaagata attacataaa aagaaacaat ccttactatc
     2401 tctgtttaac tcattatcaa tgtttatttc atcatgtaaa agtggaataa atctttcaaa
     2461 acttctatgt ctatttaaat cttttaatgt acataaagat gcgattccct ctattttaat
     2521 tgaaccactc tgtccaaggt tacctataag gctgtgttga ttgtgatttt taaaaagaac
     2581 ctccccaata tattcagaat cttcatcatc aaactctagt tcattctcgc cacctaatgg
     2641 atttattaac aactcataat gagtaaaaag gtcttctact gcattatcat tgtaactaac
     2701 agtacaggag tcactttcac tcccttgact gatatatgat ttctgaggtg aaaccttttt
     2761 ctttagatac tctaatatct ctttggtaga attttttctc gaataattag cctccgtgtg
     2821 ccttataagt ccatcagctt ctctaacata tcccgctatt tcaatatctg aatctccaag
     2881 taggctatat atcaacttcc ctacctctct atctacaata ccattgcttg cattaagata
     2941 tgatattaac tcagaccagt ttcttgcaga cattactgcg gccatacttg tctgcactcc
     3001 atatggaaga agatatcttg cacaatcaaa acttcttgcc tgtaatgcag atttttcttc
     3061 tttaatatcc tcattaatat taaaatactc cttaaatatt tcctttgtct tttttaacaa
     3121 aacagagtaa tcatgcatct gttttaaaat tatatcttca tattcctgtc taatcttttt
     3181 atcattacaa atctccctag ggattctaat atattcaggc tcttcaaaat tttgatatct
     3241 tgtagatctc tcctgaccag atattacaga gttaagataa aaaatcttca ttgcagtaaa
     3301 cattgggata ttctctatac atacaaatag ctgtgccatg tcaccaactg atttatgacc
     3361 atatccatgg aagattgctt caagtctttg ggcagcatca gtattatttt taataatctc
     3421 agatgcaata tcaattacac tatcagaaga tctgctgtat cttgcaccta aatatgcatt
     3481 gatagaaggt tgcttttgat taacattctc aagaagagta tgaaccttaa actgattcca
     3541 gtcatcttca gactctatac tcgctatttg tatatctttt ctaaatgttc ccttcatatt
     3601 cctggtatat taaatttttt actaaaatct tttacctcat ctttaattct cttaaggtta
     3661 tcctcagagc tatcataaag tgccctgtat atatactctg agatttggaa catatctttt
     3721 tctttcatac ctcttgtagt aacagcagca ctccctattc ttacacctga aggatcccat
     3781 ggttttcttt catcaaaggg tattgcattt ttgtttaatg taatatttac agactctaag
     3841 atatcggaaa cctccttgcc actcttcccg atactcttaa tcgtatcaac taaaaacata
     3901 tgattatcag taccaccaga tacaatactg aaacctttat ttgctaaaga agatgctaag
     3961 gcttttgcat tgctaattac ttgtctcgca tattctttaa actcatcact ttgagcctct
     4021 ttcaaagcta cagctatacc tgctgtaata tgattatgag gacctccttg aagccctgga
     4081 aatactgctt tatctatcag ctctccatat tcttctttac acataattat agcccctctt
     4141 gggcctctga gagttttatg tgtagttgtt gtcactacat ctgcaatccc tactggagaa
     4201 ggatacaaac ctgctgcaac aagtcctgcg acatgagcta tgtcagcaac tagtattgca
     4261 ccaatctcat cagctatttt tctaaattct ttccaatcaa ctattcttga atatgcacta
     4321 aacccactcc atatgagttt gggttcatgc tccagagcta ttcttcttac ctcttcataa
     4381 tcaatatatc cttctttatt aactccataa cttactgaat caaaatactt cccacttatt
     4441 gataccttaa atccatgtgt aagatgtcct cccgcagaaa gatccattcc cattgttcta
     4501 tctccaggtt tgaggaatgc aaaatgtaca gctaaattag caggtgaacc tgaatatggt
     4561 tgaacattta catgttctgc accaaaaagt tcttttgccc ttttaataca ttcctcttct
     4621 attttatcaa tatactcatt gcccccataa tatcttttgt gaggatatcc ttctgagtat
     4681 ttatttgtaa gtatactacc cattgcttta agtacatctt cacttacata a
//
LOCUS       DAOSAB010000019         4713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_12303_length_4713_cov_3.101760, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000019 DAOSAB010000000
VERSION     DAOSAB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12303_length_4713_cov_3.101760"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PK432_00745"
     CDS             complement(<1..256)
                     /locus_tag="PK432_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HOK59488.1"
                     /translation="MRQHAIPQNILDVEFKIFTRFTLKEFAYLGIGVGIGGVFLYFTT
                     RGQFPGIVGIPLFIFFSSIGVFLALVPINDQPADKFISNFF"
     gene            complement(308..1381)
                     /gene="recF"
                     /locus_tag="PK432_00750"
     CDS             complement(308..1381)
                     /gene="recF"
                     /locus_tag="PK432_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOK59489.1"
                     /translation="MIERLTVQNFRKFKNKTFKFNKNTVILYGHNAQGKSSVLEAIYL
                     ISTGVSPWASSNEYINLNQNSQIHARIETIENGKSYVYFKNKDKRILKIEGKNTSARK
                     FFENRSANIFNPEQIDLLMISPSERRNFLDNIILKYDYEYETLLKDFHKILRQRNAYL
                     KKLSKLFYDKGIIAVNDPQLNFWSNHFCLTSAHILKNRILLTKELSLNEYEVKYKTKI
                     DFDKRKINNIEYLKECIERDLESSKKRDIATGYTNIGPHRDDWDIFNDKDIKKFGSRG
                     EKRLAIGNLIFEIQNVVYRKTSSYPILLLDDITSELDIFNTKKILPKKLLNKQQTFIT
                     TIDFKHLPKEIIKDAQLIHLDGK"
     gene            complement(1381..3204)
                     /gene="lepA"
                     /locus_tag="PK432_00755"
     CDS             complement(1381..3204)
                     /gene="lepA"
                     /locus_tag="PK432_00755"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOK59490.1"
                     /translation="MGNIDLNKTRNFSIIAHIDHGKSTLADRILESCNVNISRDKRKS
                     RILDRLELEQEKGITIKLQACTMNWNGYELNLIDTPGHVDFSYEVSRSLAACEGAILL
                     VDASQGIQAQTISNAQKAVELGLKLIPAINKIDLPNINIKEREDELVQLLGFKREDIL
                     KVSGKTGEGVKELLNAVIEKCPPPKGDSNKALKALIFDSFYDEHKGVVVAVRIFDGRI
                     KYIPNKTKELYILQMKTSFKPTEIGIFTPTMKEANELCAGDVGYIATGFKDITMFTVG
                     DTISDSMNTLPLPGYKVPKPNVYATFFPTTADQYEPLKIGLSKLSMNDAALTFSEQRS
                     NLLGTGFRCGFLGLLHMEIVQERLEEEYDVSLIVTAPTVEYKVIKTDGTKIDIQTPQE
                     LPDPSEITQILEPWVRLKIMTPSKYMGAIMEMCQYKRGEYVNTTYLPTFSDKIQTKEQ
                     YIVLEYDIPLASLVSNFFDMLKNKSSGYASMEYEFIDFFPVDLVKVSILVNHEEIPAL
                     SFLETKENAQTKAAKLLEVMKDEIPRQQFPIPLQAAIGGKIIAREDVKGYRKDVTAKL
                     YGGDYTRKLKLLEKQKKGKKRLKQIGQVNIPQEAFLSILKI"
     gene            complement(3252..3806)
                     /locus_tag="PK432_00760"
     CDS             complement(3252..3806)
                     /locus_tag="PK432_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59491.1"
                     /translation="MKNNKLKKILSLVIEPIALGLLALLFIIPTITVMNLKPLTKKIK
                     DVDVLGLSQNAQVEITLVGGTHQIFSQEKLEQEEDGTYKYTTIITSRDANRYSKPILE
                     IKNGKSSSVKLEVYGYTDLPTHSDISLIINDQIYRIQNPQGNTITQRILLEPNQKYII
                     YLAIENFSNVKFNEDFTLEIKEIE"
     gene            complement(3806..4354)
                     /locus_tag="PK432_00765"
     CDS             complement(3806..4354)
                     /locus_tag="PK432_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59492.1"
                     /translation="MSKLLKRALKSSIFPAILMIAAKTLGIFIVSIKYGYTVEIGNDV
                     GGVFSTQLYFDNPQTVYFVNSISDLIMLLVIALPTAYQVIKVVLFQSTFTNPRTIVKA
                     AQFNILKWVTKDDTTFVQIFVWCIFLWLCSGVVIKNALQGDTYTWIAYLAGVFSLLCG
                     LGILKAFEIEINRVYPDSKKYY"
     gene            complement(4326..4622)
                     /gene="rpsT"
                     /locus_tag="PK432_00770"
     CDS             complement(4326..4622)
                     /gene="rpsT"
                     /locus_tag="PK432_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOK59493.1"
                     /translation="MPNLKTSIKDLRSTKRKTVYNDRLRKRIKRAEKNFKDSIKEGDR
                     EKAEASLKQTYKVLDKAAKKNVIKKGKASRKKSRLASQLNKLSKDNVKTTQKSA"
BASE COUNT         1351 a          763 c          689 g         1910 t
ORIGIN      
        1 aaaagaaatt agatatgaat ttgtcagcag gttggtcatt tattggaaca agtgctaaga
       61 ataccccaat gcttgaaaag aaaataaata gaggaatacc tacaatacca gggaactgtc
      121 ctctagttgt aaagtacaaa aatacccccc ctatgcccac tcctatccct aggtaagcaa
      181 attctttaag cgtaaaccta gtaaagattt taaactcaac atctaaaatg ttttgtggta
      241 tagcatgctg tctcatattc cgtatatcta gtttatcaga attaaaagaa atagtggaga
      301 tattatatta ctttccatct agatgtatta attgtgcatc ttttataatt tcttttggta
      361 aatgcttaaa atcaattgtt gtaataaagg tctgctgttt gtttaataat ttcttaggca
      421 gtatcttttt tgtattgaat atgtctaatt cagaagtaat atcatcaagg agaagtatag
      481 ggtaacttga tgtttttctg tatacaacat tctgtatttc aaagattaaa tttcctattg
      541 ccaatctctt ttctcctctt gatccaaatt ttttaatatc tttgtcatta aatatatccc
      601 aatcatctct gtgcggacct atatttgtat atcctgttgc tatatctctt tttttactag
      661 attccaaatc tctctctata cactctttaa ggtactctat attgtttatt tttctcttat
      721 caaaatctat ttttgtttta tactttacct catattcatt tagggacagc tcttttgtta
      781 aaagtattct gtttttaagt atgtgtgcag atgttagaca gaaatgatta ctccagaaat
      841 taagctgagg atcatttaca gcgattattc ctttatcgta aaagagcttg gagagttttt
      901 ttaaatatgc atttctttgt cttaatattt tgtgaaaatc ttttagaagt gtttcatact
      961 catagtcata ttttaatatt atattatcaa ggaagtttct tctttcagaa ggagatatca
     1021 ttaataggtc tatttgttct gggttaaata tatttgcaga tctgttttca aaaaatttcc
     1081 tagcacttgt attttttcct tctatcttta aaattctttt atctttgttt ttaaagtata
     1141 catacgattt cccattttct attgtttcta tacgtgcatg tatctggcta ttttgattta
     1201 agttaatata ctcattagat gatgcccatg gagatactcc tgtactgatt aaatatatag
     1261 cttcaagtac agagctttta ccttgtgcat tatggccgta aaggataact gtgtttttat
     1321 taaacttaaa ggttttgttt ttaaactttc taaaattttg aactgttaat ctttcaatca
     1381 ttatattttt aatatactca aaaatgcttc ctgaggaata tttacctgtc ctatctgttt
     1441 taatcttttt ttacctttct tttgtttttc aagaagtttt aatttccttg tatagtctcc
     1501 tccatatagt ttagcagtga catcttttct atatcctttt acatcctctc ttgctattat
     1561 tttacctcct attgcagctt gaaggggaat agggaactgt tgtcttggta tttcatcttt
     1621 cataacttct agaagttttg ctgcttttgt ttgtgcattc tcttttgttt caagaaaact
     1681 taatgctggg atttcttcat gattaacaag tattgaaact ttaacaaggt ctactggaaa
     1741 gaaatctata aactcatact ccatagaagc atatccgctt gatttgtttt ttagcatatc
     1801 aaaaaaattg gatactaatg atgctaaggg aatatcgtat tctaggacga tatactgttc
     1861 cttagtttgt attttatctg aaaaagtagg taggtatgtt gtattaacat actctcctct
     1921 tttatactgg cacatctcca ttattgcacc catgtattta cttggtgtca tgattttaag
     1981 tcttacccat ggttccagta tttgggtgat ttcagagggg tcaggtaatt cttgtggtgt
     2041 ctgtatgtca atttttgtcc catctgtctt tatcacttta tactctactg ttggtgctgt
     2101 aactattaaa ctcacatcgt actcctcttc taatctctct tgtactatct ccatatgtag
     2161 taggcctaag aatccacatc taaatcctgt acctaaaaga tttgatcttt gttcagagaa
     2221 tgtaagtgca gcatcgttca tgcttaattt gcttaaccct atttttaaag gctcatattg
     2281 atcagcagtt gtggggaaaa aagtagcata tacatttggt tttggtactt tataccctgg
     2341 aagtggtaat gtattcatgc tatctgatat tgtatctcct actgtaaaca ttgttatatc
     2401 tttaaatcca gtagctatat atcccacatc tccagcacat agctcatttg cttctttcat
     2461 tgttggagta aatattccta tttctgttgg tttaaaggat gtcttcatct gaaggatata
     2521 taactctttg gttttgtttg gaatgtactt tattcttcca tcaaatattc ttacagcaac
     2581 aaccacaccc ttatgctcat cataaaaaga gtcaaatatt aatgctttga gtgctttatt
     2641 agaatcacct tttggaggag gacatttctc aattactgca ttaagtaatt cttttacacc
     2701 ctctcctgtt tttccagata ctttaagaat atcttctctt ttaaaaccaa gaagttgtac
     2761 aagttcatct tctctttctt ttatatttat attaggtaaa tctattttgt ttattgcagg
     2821 aattaatttt aatcccaact caactgcttt ttgtgcattg gatattgttt gagcctgtat
     2881 accttgagaa gcatcaacaa gaagtatcgc tccttcacat gcagcaagtg agcgtgatac
     2941 ttcatatgag aagtcaacat gacctggagt gtcaatgaga ttgagttcgt atccattcca
     3001 attcatggta catgcctgga gttttattgt aattcctttc tcttgttcta gttctaatct
     3061 atcaagtatt ctagactttc ttttatctct ggatatatta acattgcacg actctaatat
     3121 cctgtctgct agtgtagatt tcccatgatc tatatgtgca attattgaga aattccttgt
     3181 tttatttaaa tctatattac ccataatatg ggtaattata ttatatttac aatacaaaag
     3241 gatactatat attactcaat ctcttttatc tcgagtgtaa agtcctcatt aaactttaca
     3301 tttgagaagt tttcaatagc aagatatatt atgtacttct gattaggttc cagaagaatt
     3361 ctttgtgtta ttgtattccc ttgaggattt tgaattctat atatctggtc attaattatt
     3421 aaagaaatgt ccgaatgtgt aggaaggtct gtatatccat atacctctag ttttacactt
     3481 gatgatttac cattttttat ttcaagtata ggtttggagt atctattagc atctctgctt
     3541 gtgataattg tagtgtattt gtatgttcca tcttcttctt gttcaagctt ttcctgacta
     3601 aatatttgat gtgttcctcc tacgagagtt atctctactt gtgcattttg acttaaacca
     3661 aggacatcaa catctttaat cttttttgta agaggtttta aattcattac tgttatggta
     3721 gggattataa aaagaagtgc aagtaatccc aatgctattg gttcaataac taaagaaaga
     3781 atttttttaa gtttattgtt tttcattagt aatatttctt actatcagga tataccctgt
     3841 ttatttctat ttcaaatgct tttagtatac ctaatccgca gagaagtgaa aatacccctg
     3901 caagatacgc aatccatgta tatgtatctc cttgaagtgc atttttaatt actacaccac
     3961 tacaaagcca aaggaatata caccatacaa agatttgaac aaaggttgta tcatcttttg
     4021 ttacccactt taagatgtta aattgagctg ctttaactat tgttctaggg ttagtaaatg
     4081 tgctctggaa aagaactacc ttgataacct gatatgcagt gggtagtgct attacaagaa
     4141 gcattattaa atctgatata gagttaacaa agtatacagt ttgaggatta tcaaaatata
     4201 actgagtcga aaatactcct ccaacatcat tacctatctc tacggtatat ccgtatttta
     4261 tagaaacaat aaaaattcct aatgttttag ctgcaatcat taatattgca ggaaatatgg
     4321 aacttttaag cgctcttttg agtagttttg acattatctt tggacagttt attaagttga
     4381 cttgcaagtc ttgatttctt tcgacttgct ttaccttttt ttataacatt tttcttagct
     4441 gctttgtcaa gaactttata agtttgtttt aaagaagctt ctgccttttc tctatctcct
     4501 tcttttatgc tatctttaaa attcttttct gcccttttta ttctttttct tagtctgtca
     4561 ttataaacag tttttctttt tgtacttctt aggtctttta ttgatgtttt tagatttggc
     4621 atgatatgta aaaatatatt attaaatcat tgataactgt gttgtattat atatataact
     4681 ggaatgtttg taaacagtag attttattga tac
//
LOCUS       DAOSAB010000020         4697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_12363_length_4697_cov_2.882809, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000020 DAOSAB010000000
VERSION     DAOSAB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12363_length_4697_cov_2.882809"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..619)
                     /locus_tag="PK432_00775"
     CDS             complement(212..619)
                     /locus_tag="PK432_00775"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HOK59494.1"
                     /translation="MSNEEYMYKSKKTIKSAISFISMFFAPALVFAKSTTTTTPMSWV
                     DEIFDSLLSTPGTLEDLIVKVLNWLIGGSAVVCVVMLIVAGFTYMTSGGNEDKTQKAT
                     KTLTNAIIGLVICFIAVMLVNFVLKNFLVTNTK"
     gene            complement(628..1011)
                     /locus_tag="PK432_00780"
     CDS             complement(628..1011)
                     /locus_tag="PK432_00780"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HOK59495.1"
                     /translation="MIGVLLFSSVSLTFALSVGEILSGFEGTGGSLSDFIVTILNWLI
                     GAAGVICVVMIIVGGYSYMTAGGDESKVKDASKTLTNAIIGLVICFIAVILVNYVLEN
                     FLTPNTPPTPTPPPAPGPGPTPVPM"
     gene            complement(1056..1409)
                     /locus_tag="PK432_00785"
     CDS             complement(1056..1409)
                     /locus_tag="PK432_00785"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HOK59496.1"
                     /translation="MKKKDLKNKRDKTKWMFLPVIIKLGDWEIDFPSFNEQFTTVEGI
                     VDFVIDLLNFLVGASAVVAVGLIIYGGITYMTAGGDPDNVKKASNILTATIIGLCIVF
                     LARMLIKFVLDTFLL"
     gene            complement(1411..1734)
                     /locus_tag="PK432_00790"
     CDS             complement(1411..1734)
                     /locus_tag="PK432_00790"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59497.1"
                     /translation="MRQNYKIYASQITEKVGELISGIEKSNPGETNVESIMQDIIDIA
                     LPLSVLAAALLMAFAAYKMITSQGNPDKLKDAREQIGNAITGLVFILVSAALLLLIGG
                     IFLPK"
     gene            complement(1777..>4697)
                     /locus_tag="PK432_00795"
     CDS             complement(1777..>4697)
                     /locus_tag="PK432_00795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOK59498.1"
                     /translation="ALTFTEKAADEMLQRVDESMPIGYGDVWISTFHSFCDRILKQEG
                     YNIGLDTDYILMSAAQSYVFLRKHLFDLSLKKFRPYGNPTKFVNDLLDHFSRLQDEDV
                     SPSEYLEYARSLSRKDSVEKEEYEDTLELATVYDEYTKLKMKNSMLDFGDLILLTIKL
                     FREKPNVLKRYQEKFKYILVDEFQDTNYSQSVLVNILSGLDPKKDMDKVGKVNPNLTV
                     VGDDDQAIYKFRGAAISNILQFKQYYPKAKKIVLTENYRSHQKILDSAYGLIKNNNPY
                     RLEVTEKVDKRLIAMESFPFDDDVVNLITAESEDAEGEWIAKEILSLTGNSDETSVRG
                     TQKFNESGQSAFLELVPDRRYKLSDIAILVRANAHADSIVQNLRYFGVPYNLGGSRGL
                     YFREEIKVLISFLKALTDYTDEVSMYMVLSMQEWGLSTREFLEINRIAREDHVSVLEE
                     LEGMFGVSIGNSMEFAVSNLGKKIFSESSREGIKRFLTVYDKCAQMIKEKKSVGEILL
                     YFINESGYINSLLEEEGPLAEFKANNLARFFNTIKEYERANPDSNVYEYVDFLNYSIE
                     VGDSPLVDQMDLNEYDAVNVLTVHSAKGLEFPVVFLVNLVADRFPSQNRRDTIPIPQV
                     LIKETLSGLDEREEHLAEERRLFYVGATRAKEKLYLTAANFYGNAKRKKKPSIFLNEI
                     LDRDVTEEFDNPMAVKSDDGLNVYKEETKNIVPEDVKMEVGDKVSYSQIDTYIDCPKK
                     YEYAYVLQIPTLPHASLSFGSTIHKTLKDFYTLLQRYKEGLGITNPPTEGELIEIFNK
                     NWISKGYENKKHEEDRKKSGIKAMKEYYKKFFSTEQNPYRLEQPFTVHLPEGSFVGKI
                     DRIDLVKAGDIPEVEIIDYKTGHLKDEADIKNDLQLPLYCIFAEQSLGVKVKRAKYIF
                     IEEGVEIEVDISEERREKAKESVYEIVRRIKDREFTATPSSFKCKYCDFRSICKDAIL
                     "
BASE COUNT         1560 a         1002 c          557 g         1578 t
ORIGIN      
        1 accctgtata tcatcccaaa gacttgacac tccgcctaca cctacaccac caccagcacc
       61 aggacctgga ccgacaaaag ccatcatagg aaatgataaa agaacaactc ctgatgtaaa
      121 aatctttgaa agtttattct tcatatatat ttaagtataa ataaattaat taagttatta
      181 tagtatatct tcttaaaaag aacaattatc tttattttgt attagtaact aaaaaattct
      241 ttaatacaaa gtttaccagc attacagcta taaaacaaat aactaaacca ataattgcat
      301 ttgttaatgt ctttgttgct ttctgtgttt tatcctcatt accacctgaa gtcatatatg
      361 taaatcctgc aacaattaac ataactacac atacaacagc agaaccccca attaaccagt
      421 ttaatacctt aacaattaaa tcctcaagtg taccaggagt agaaagcaaa ctatcgaaga
      481 tctcatctac ccatgacata ggggtagttg tagtagtact cttagcaaaa accaatgccg
      541 gtgcaaaaaa catagagata aaggaaattg cactttttat tgttttttta ctcttataca
      601 tatactcttc attactcata ttacatatta cataggaaca ggagtcggtc caggtcctgg
      661 tgctggtggt ggtgtaggtg taggcggagt gttaggtgtt aagaaattct caagaacata
      721 attaaccaat attaccgcaa taaaacaaat tactaaacca ataattgcat ttgtcaatgt
      781 ctttgatgca tccttaacct tactttcatc cccacctgct gtcatatatg aatatcctcc
      841 gacaataatc attacaacac atattacacc agcggcacca ataagccaat ttaaaatagt
      901 aacaataaaa tcacttaaac ttccccctgt cccttcaaaa ccacttaaga tttctccaac
      961 acttaatgca aaagttaacg aaacagaact aaaaagtaaa actccaatca cagttatttt
     1021 agataaactt tttttcactt ttaaatattt ttaatttata aaagaaatgt atctaataca
     1081 aattttatca acatcctagc caaaaataca atacatagtc caataattgt agctgttaaa
     1141 atgttgctag cctttttcac attatcagga tcaccaccag cagtcatata tgtaatccct
     1201 ccataaatga ttagaccaac agcaacaaca gcagatgctc caacaaggaa attcagcaag
     1261 tcgataacaa aatcaacaat accctcaaca gtagtaaact gctcattaaa agagggaaaa
     1321 tctatctccc aatcccctaa cttaataata acaggaagga acatccattt tgttttatct
     1381 cttttgtttt ttaaatcttt ctttttcata ttatttaggt aaaaatatac cccctattaa
     1441 taacaaaaga gccgcagaaa ccaaaataaa gacaagaccc gtaatagcat tccctatctg
     1501 ctctcttgca tccttaagtt tatcaggatt cccctgactt gttatcatct tgtatgcagc
     1561 aaaagccata agaagtgcag ctgcaagaac actgagaggt agtgcgatat cgattatatc
     1621 ctgcattata gattctacat ttgtctcccc tggattactc ttctcaattc cactaataag
     1681 ttctcccact ttctcagtaa tctgactagc atatatttta taattctgtc tcataaacat
     1741 gcaattatac taaaaaatct acaaattaaa aacaatttat aaaatagcat ccttacatat
     1801 agatctaaaa tcacaatatt tacacttaaa actgcttgga gttgcagtaa attctctatc
     1861 tttaatcctt cttactatct catacacact ctcttttgct ttttctcttc tctcttcaga
     1921 aatatccact tctatctcta caccctcttc tataaatata tactttgctc ttttaacttt
     1981 tacaccaaga ctttgctctg cgaaaataca gtacaaagga agctgtaaat cattctttat
     2041 atctgcctcg tcttttaaat gacctgtttt ataatcaatt atttctacct ctggaatatc
     2101 ccctgctttt accaaatcta ttctatcaat ctttccaaca aaacttccct ctggaagatg
     2161 cactgtaaat ggctgctcca acctgtatgg gttctgctct gtactaaaga actttttgta
     2221 atactctttc attgctttaa taccactctt ttttctatcc tcttcatgct ttttattttc
     2281 atatccctta cttatccaat ttttattaaa aatctctatt aactccccct ctgttggagg
     2341 gtttgtaatt ccaagtcctt ctttgtatct ttgaagaagg gtataaaaat ctttaagagt
     2401 tttatgaata gtagatccaa aagacaaaga tgcatgcggc aatgttggta tctgcaaaac
     2461 atatgcatac tcatactttt tagggcagtc aatatatgta tctatttgag aataactgac
     2521 tttatctcct acttccattt ttacatcttc aggaacaata tttttagttt cttctttata
     2581 aacattcaaa ccatcgtcag atttaacagc cataggatta tcaaactctt ctgttacatc
     2641 tctgtcaagt atttcattta aaaatatact aggcttcttt tttcttttag catttccata
     2701 gaaattagca gcagtaagat acaatttttc tttagcacga gttgcaccta cataaaataa
     2761 tctcctctct tctgctaaat gctcttctct ttcatctaaa ccagataatg tctcttttat
     2821 aagcacctga ggtattggaa ttgtatctct cctgttttga gaagggaacc tgtcagctac
     2881 cagattaact aagaatacaa caggaaactc caatcctttt gcactatgaa cagttaaaac
     2941 atttacagca tcatattcat ttaaatccat ctgatctacc agaggagaat caccaacttc
     3001 tatactgtag tttaagaaat ctacatactc atatacattt gaatcaggat tagcacgctc
     3061 gtattcttta atggtattaa aaaatctcgc aagattattt gctttaaact ctgctaacgg
     3121 tccttcctct tctagaaggc tattaatata tccactttca tttataaaat aaagaagaat
     3181 ttcaccgaca gatttcttct ctttaatcat ttgagcacac ttatcatata ctgttaaaaa
     3241 tcttttaata ccttctcttg aactttctga gaatatcttt ttaccaaggt tgctaactgc
     3301 gaattccata ctgtttccta tacttacccc aaacatacct tcaagctctt ccaaaactga
     3361 cacatggtcc tcccttgcaa ttctgttaat ctctagaaat tctcttgtag acaaacccca
     3421 ctcttgcata gaaagaacca tatacataga cacttcatct gtataatctg ttagggcttt
     3481 taagaaagat ataagaacct taatttcctc tctaaaatac aaacctcttg aaccacccag
     3541 attataagga acaccaaaat accttaagtt ctgcacaata ctatctgcat gagcatttgc
     3601 tctaaccaat attgcaatat ccgacagttt atatcttcta tcaggaacaa gctcaagaaa
     3661 cgcagactgt ccactttcat taaacttctg agtacctctg acagatgtct cgtctgaatt
     3721 ccccgtcaat gataatatct cttttgctat ccactctccc tctgcatctt cactctcagc
     3781 agtaattaaa ttaacaacat catcatcaaa aggaaatgac tccattgcaa ttaacctttt
     3841 atcaaccttc tcagttacct ctaatctgta tggattatta ttcttaatca agccgtatgc
     3901 actatcaagt atcttctgat gagatctgta attttctgtt aaaacaatct tttttgcctt
     3961 aggataatac tgcttaaatt gcagtatgtt tgaaatagct gcacctctaa atttataaat
     4021 agcttgatca tcatcaccca caacagttaa attaggattt acctttccta ctttatccat
     4081 atctttttta ggatctaacc cacttagtat atttactagt acactctggg aatagttagt
     4141 atcttgaaac tcatcaacaa gaatatattt aaacttctct tggtatctct ttaatacatt
     4201 aggtttttct ctaaagagct taatagtaag aagtattaaa tcaccaaaat caagcataga
     4261 gtttttcatc ttaagctttg tatactcatc atatactgtc gcaagttcta aagtgtcctc
     4321 atattcttct ttctcaacag aatcttttct tgaaagagat cttgcatatt ccaaatactc
     4381 tgatggagaa acatcttcat cctgaagcct tgaaaaatgg tccaacaaat catttacaaa
     4441 cttagtggga tttccatatg gcctgaattt ttttagagag agatcaaaaa gatgcttcct
     4501 taaaaacaca tatgattgag cagcagacat taagatataa tccgtatcta aaccaatgtt
     4561 atatccttcc tgctttagta ttctgtcaca aaaagaatga aaggtggata tccagacatc
     4621 accataccca ataggcatac tctcatctac cctttgcaac atctcatcag ccgctttttc
     4681 tgtaaatgta agagcaa
//
LOCUS       DAOSAB010000021         4686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_12405_length_4686_cov_3.437487, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000021 DAOSAB010000000
VERSION     DAOSAB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12405_length_4686_cov_3.437487"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PK432_00800"
     CDS             complement(<1..601)
                     /locus_tag="PK432_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59499.1"
                     /translation="MDKKEKYQKFLKIYQTLGFILIFITIVLIAIPTAPYVWYRLNPN
                     AVQKDEEKIVQNVVEAKEEIPQEPEPVEEKIPPINPNLPEGYFVVIEEIGVNSPITIT
                     KDYRKALTKGTWIVNDFGTPENDDLPIILAAHRFGYSSWGTKKRNLISFYNLPKTGPG
                     TKVKIYWNQREYIYEIYDGNVGTYITDYNADLILYTCKYY"
     gene            733..972
                     /locus_tag="PK432_00805"
     CDS             733..972
                     /locus_tag="PK432_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59500.1"
                     /translation="MKEYINKILGGNTMKNNNVQIEDIMVLIDQYFKILDKKISENEK
                     EILLLFKEIKKAKTKADRINEFDSGEIKIVSYLDN"
     gene            complement(975..3623)
                     /locus_tag="PK432_00810"
     CDS             complement(975..3623)
                     /locus_tag="PK432_00810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOK59501.1"
                     /translation="MKSNYIDYSMSVIVSRALPDVRDGLKPSQRRVLVAMNDLGLTPT
                     SHFRKCAKIAGDTSGNYHPHGESVVYPTLVKMAQEFSTRYILVEGQGNFGSIDGDPAA
                     AMRYTEARMGKITPEMLRDLNKGTVTFIPNYDGTRLEPTVLPTLFPNLLANGSQGIAV
                     GMATQIPPHNLTELVDALREMIKRGNKWQGVAIYNELRKARESREKVPQTLNAKPENY
                     LENYVDTKSLDYNKEIEKILERIKETKETIYPDFKSDISPEELIEIIPGPDFPTGGTI
                     YDRAEILNAYATGRGRILQRAQASIVEGKNGRYQIVITEIPYQVNKAYMIEKIADLVR
                     EKKITGIADIRDESNKDGIRVVVALKKDAQPKTVLNKLYKYTEMQKTFNANMIALVDQ
                     EPITLSLKRMLELFLSFRITVTIRRYEYDLAEARYHAHILEGLLKALDLLDEVIATIR
                     GSKTQESAKTNLMDKFKFTDVQAQAILDMQLRRLAALERMKLQNDYKETKEKIIQYNE
                     ILDSQDKILEVISNDLEVMKETYGDKRRTKIVKGKINEISDEDLIAQEETLITITHTG
                     YVKRVPPSTYQTQKRGGKGISGGETKEGDFIEHVLLCNTHDELLLFTNKGRVFSTRIF
                     EIPEYGRTAKGIPLINLVQLDQNEIITSILTRDPKGGVMGSEEIQEQQDEKGVIKKAD
                     FKYFLMVTKGGIIKKTDLKQFSKIRANGLTAIKLEPGDELIWVKPTTGDDNVILVTAE
                     GKSIKFSETDVRPLGRNTRGVIAIKFKTDTDYVVGMGVIRKNEDKLFTLSEYGHGKMT
                     KLSEYPLQKRGGTGVFTFRVTPKTGKVVAARVLDHPNAEIVVISEKSKVIRSSIKAIP
                     TLGRQTSGVKVMNLNTGDKVATMALL"
     gene            complement(3790..>4686)
                     /locus_tag="PK432_00815"
     CDS             complement(3790..>4686)
                     /locus_tag="PK432_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOK59502.1"
                     /translation="KIKLNNPEVKTAVAKVIRDELLTYLDEHPKEARAIIDKAVLSYK
                     AKAAAKAARDAVIRKNALESTTLPGKLADCISNDPRECELYIVEGDSAGGSAKQGRDR
                     HTQAVLPLRGKIINTNKYRVDRVLGNNEFKDITTALGVGIGASLDVSKLRYHKIIIMS
                     DADVDGLHITTLVLTLLYRFFKPLIEQGYVYVAQPPLHKVEIGKKKYYFLNDEEKDAF
                     VRKAKTEGKTPVANRFKGLGEMNPDQLWETTMNPETRVLKRVQIEDAQEAEKTFEMLM
                     GTEVPPRRRFIQKYAKKANLDI"
BASE COUNT         1272 a          852 c          686 g         1876 t
ORIGIN      
        1 tataatattt acaagtgtat aaaattaaat ctgcattata atcagttata tatgttccaa
       61 cattgccatc atatatttca tatatatact ccctttggtt ccaatatatc tttacctttg
      121 ttccaggacc tgttttcgga aggttataaa aggatattaa gttcctcttt tttgttcccc
      181 agcttgaata tccaaacctg tgtgctgcca gtattattgg caggtcatcg ttttcagggg
      241 taccaaagtc atttacaatc catgttcctt ttgtaagtgc ttttctataa tcttttgtta
      301 ttgtaattgg tgaatttacc cctatttctt caattactac aaagtatcct tctgggagat
      361 taggattaat agggggtatt ttttcttcta cgggttcagg ttcttgtgga atttcttctt
      421 ttgcttcaac aacattttga actattttct cttcatcttt ttgtactgca ttaggattaa
      481 gtctatacca tacatatgga gcagttggta tagcaatgag gacaatagta ataaaaatca
      541 gaataaagcc taatgtttga tatattttta ggaacttttg atatttctct tttttatcca
      601 tactttatag attatcacat atatatggcg aagagaaaag ctcaagggtg taatgaataa
      661 cttaaaattt tatagtgata aaaatataac ttgaagatat tggatattag gtcaagaaat
      721 atattttaga atatgaaaga atatattaat aaaatattag gaggaaatac tatgaaaaac
      781 aacaatgtgc aaatagaaga tataatggta ttgatagacc aatattttaa gatacttgat
      841 aaaaagatat ctgaaaatga aaaagagata ctgctgctct ttaaagagat taaaaaagca
      901 aaaacaaagg cagatagaat taacgagttt gactcagggg agataaaaat agtgtcatat
      961 ttagataatt aaacttacaa taaagccatt gttgcaacct tatctcctgt atttagattc
     1021 ataaccttaa caccacttgt ttgtcttcct aatgtaggaa tggctttaat agaagatctg
     1081 ataacttttg atttttcaga tattacaact atttctgcat taggatgatc aagtactctt
     1141 gcagctacaa ccttacctgt tttcggtgtt actctgaatg taaatactcc tgttcctcct
     1201 cttttttgta gtgggtactc tgaaagtttt gtcatttttc catgtccata ttctgaaagg
     1261 gtaaatagtt tatcttcatt ttttcttatt actcccattc ctacaacata atctgtatca
     1321 gttttgaatt ttattgctat aacacctctt gtgtttctac caagtggtct tacatccgtc
     1381 tcactaaatt ttattgattt tccttctgct gtaactaata ttacattatc atctccagta
     1441 gtaggtttaa cccatattaa ctcatcacct ggttctagtt ttattgcagt taagccattt
     1501 gctctaatct ttgagaattg ttttaaatca gtttttttaa taattcctcc ctttgttacc
     1561 attaagaaat acttaaaatc tgcctttttt attacacctt tttcatcttg ttgctcttgt
     1621 atctcttcag aacccattac tccaccctta ggatctcttg taagaataga agttattatt
     1681 tcattttgat ctaattgaac aaggttaatt aaaggaatac cctttgcagt tcttccatat
     1741 tcaggtattt caaatattct tgtactaaat acccgaccct tgtttgtaaa tagcagtagt
     1801 tcatcgtgtg tgttgcaaag caatacatgc tcaataaagt caccttcttt tgtttcacca
     1861 ccagatattc cctttcctcc cctcttctgt gtctgatatg ttgaaggagg tactctcttt
     1921 acatatccag tatgggttat tgttatcagt gtttcctcct gtgcaattaa atcttcatct
     1981 gaaatttcgt ttatttttcc ttttacaatc tttgttcttc tcttatctcc ataggtttct
     2041 ttcattactt ctaagtcatt gctaattact tcgagtattt tatcttggct gtctaaaatt
     2101 tcattgtact ggataatctt ttcttttgtt tctttataat cattttgcag tttcattctc
     2161 tcgagagctg caagtcttct aagttgcata tctaatattg cttgtgcttg tacatcagtg
     2221 aacttaaatt tatccattaa gtttgttttt gcactctcct gagttttaga tccccttata
     2281 gtagcaatta cctcatctaa aaggtctaat gctttaagta atccttcaag aatgtgtgca
     2341 tgatatcttg cttctgccaa atcatattca taccttctaa tagttacagt gattctaaaa
     2401 gaaaggaaca actcaagcat tctctttagt gaaagagtaa tgggttcttg atctaccaat
     2461 gcaatcatat ttgcattgaa ggtcttctgc atctcagtat atttatacag tttgtttaat
     2521 actgtttttg gctgtgcatc tttcttaaga gcaacaacca ctcttattcc atctttgttt
     2581 gattcatctc tgatatcagc aattcctgta attttctttt ctcttaccaa gtctgcgatt
     2641 ttttctatca tgtaagcttt atttacttga tatggaattt ctgtaattac aatttgatat
     2701 ctcccatttt ttccttcaac tattgatgct tgtgctcttt gaagtatcct tcctctcccc
     2761 gttgcatatg cattaagtat ttcagctcta tcatatattg ttcctccagt aggaaaatct
     2821 ggtcctggaa ttatttcaat taattcttct ggagatatat ctgacttgaa atcagggtat
     2881 attgtttctt ttgtttcttt aatcctttca agtatctttt caatctcttt gttataatcg
     2941 agagatttag tatccacata attttctaag taattttcag gtttagcatt aagtgtttgt
     3001 ggtacttttt ctctgctctc tcttgctttt ctaagttcat tgtaaattgc tactccctgc
     3061 catttgtttc ctcttttaat catttctcgg agtgcatcta caagctctgt aagattatga
     3121 ggaggtattt gtgttgccat tcctactgca ataccttgac ttccatttgc taaaaggtta
     3181 gggaaaagtg tgggaagtac tgttggttca agtcttgttc catcatagtt aggtataaat
     3241 gtaacagttc ctttatttaa atcacgaagc atttctggag taatttttcc cattcttgct
     3301 tctgtgtatc tcattgccgc tgcaggatct ccatcaatag aaccaaagtt cccttgtcct
     3361 tcaactaaaa tatatctagt tgaaaactct tgagccattt ttacaagagt tggatatact
     3421 acactttcac catgtgggtg atagttacct gaggtatcac cagcaatttt tgcacacttt
     3481 ctaaaatgag aagttggtgt tagacctaag tcattcattg cgactagtac tcttctttga
     3541 gatggtttta atccatctct tacatctgga agtgcacgag agactataac ggacatagag
     3601 tagtcaatgt agtttgattt catctcatca acaatggacc tttcttttat ctctccgggt
     3661 ttaaaagata gattaaaatc aatttctgaa ttattttgct ctgcttcagt tgagttttct
     3721 ggatcttgat tttcaatatt tatactttct tgtgcacttt ttattaaatc ttctttatct
     3781 gccatgtctt tatatatcta aattagcttt ttttgcatat ttttgaataa atcttcttct
     3841 tggaggaacc tctgttccca ttaacatttc aaaagttttt tcagcttctt gagcatcttc
     3901 tatctggact cttttgagaa ctcttgtttc tggattcata gttgtttccc aaagctggtc
     3961 tggattcatt tctcctagac ctttaaatct gtttgctact ggagtttttc cttcagtttt
     4021 agcctttctt acaaaagcat ctttttcttc atcgtttaga aagtaatatt tctttttgcc
     4081 aatttctacc ttatgtagtg gaggctgtgc aacatataca tacccttgtt ctattagagg
     4141 tttaaagaat ctgtaaagaa gggtaagtac taaagttgtg atgtgtaaac catcaacatc
     4201 tgcatcactc attattataa tcttatggta tcttaatttg cttacatcta aagatgcccc
     4261 tattcctact cccagagctg ttgtaatgtc tttaaactca ttattaccta aaactctatc
     4321 cactctgtat ttattagtgt taataatctt acctcttagt ggaagtactg cttgtgtatg
     4381 tctatctctt ccttgctttg cacttccacc tgcggaatct ccctctacaa tgtatagttc
     4441 acactcacga gggtcattgc ttatacaatc tgcaagttta cctggcaatg ttgtactttc
     4501 taatgcattc tttctgatta cagcatccct tgcagccttt gctgctgcct ttgctttata
     4561 tgaaagtaca gctttatcaa taatagctct tgcttcctta ggatgttcat caaggtatgt
     4621 taaaagttca tctctaatta cttttgcaac agcagtttta acttctggat tatttaattt
     4681 tatttt
//
LOCUS       DAOSAB010000022         4664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_12482_length_4664_cov_3.435235, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000022 DAOSAB010000000
VERSION     DAOSAB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12482_length_4664_cov_3.435235"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="PK432_00820"
     CDS             <1..1204
                     /locus_tag="PK432_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59503.1"
                     /translation="YLAQGSFHTTDKLGSSFHGGYQETVKTKGINPKYVSKFYVTEPG
                     TALNDVWTVCAEKNCGLECETTYRLRVDIKGSPVLRTLTRNMYFTVDAYTGCCDDPDN
                     PSAVDPNVVLLQWADRINEYPLTSPFVKAFVMNNPVEVEVAYAEASTLVTDDATVVPV
                     AGQVVVGEGIPVGAYVGTVTPVGGSPITAYKFTVVDVDGNLLEIANDYDNSVELYTII
                     DSETYVPATTSADVANIVSCLVVTGAYVDTTFGDCSFSPCDKYEIEPVHINLSVVDET
                     GDPCVVSCLTVTERQNAYQGKGYGEPLLREYILSKRYQQEPWFDDVRLREINDNTTMT
                     DINRSGKYYVYHILHSVPRKSNSDGMLDNDQYLVKIVVKARDADFEAWMNAYLASANN
                     GVQLEVVI"
     gene            1306..1863
                     /locus_tag="PK432_00825"
     CDS             1306..1863
                     /locus_tag="PK432_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59504.1"
                     /translation="MAIKHRLALEILDTACDHALKISDMSSYGQGLPVECPRLDITLP
                     GFYQPVYLNNLTPGFNEIITMITLGYQEANATELNGFPDGLYTIRYSVSPNDKVFVIY
                     NHLRTTCITNEYYRELCKLQLAECEPSTEIKQKLNDLRYIKMLIDAAKAKAEYCDSCV
                     QATDMLGYAQRMLSKYQSGNCLTCN"
     gene            1870..2007
                     /locus_tag="PK432_00830"
     CDS             1870..2007
                     /locus_tag="PK432_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59505.1"
                     /translation="MASTCVCGNKLGCSCQRRRASDGTMRCTKCIAAYEAKLKQLKLK
                     K"
     gene            2004..2249
                     /locus_tag="PK432_00835"
     CDS             2004..2249
                     /locus_tag="PK432_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59506.1"
                     /translation="MIYDYIENTNLEKISELFVHSIQEDYIKRRFGIKSCKNIIDYNY
                     AYDILNLYKRALERDACNICDESYCCTNQIEERIKTL"
     gene            2251..3054
                     /locus_tag="PK432_00840"
     CDS             2251..3054
                     /locus_tag="PK432_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59507.1"
                     /translation="MSNCVPTSSNCIIWQGPNIECIGLCQGDTITDVIYKLATAYCEL
                     SNQLQADNFDISCIECPEGIDCTVDNIQDLLDILIAKICELQTSVGTPGEQGIQGRSG
                     PQGPPGPQGPAGPQGPQGPQGEPGVTGATGPQGPQGPQGPIGPQGPRGERGPAGPQGE
                     QGPQGEPGECPCCNFRVKITWYDPGPYDARYVGTVYGGTPPYTYMWRLKEGPISNLTL
                     VYPDSIPSRCGIYQLNPKNGIGLIEVEVTDANGCKAFDTILYINLSNII"
     gene            3056..4651
                     /locus_tag="PK432_00845"
     CDS             3056..4651
                     /locus_tag="PK432_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59508.1"
                     /translation="MALSKNCTPVSSSCVIWNGPKIECIDQCQNDSIEDIVYRLGQII
                     CSLSEMSQSDCNCDNKIDNLLYNCLITDQQIPQWGEGGKGWTGQQLLEIYAEELCSRA
                     NGNVTVQDLVTLHKKINQPLALKDHLQYEEDGQTITELTYDQYILLLAQWVCDLYDTL
                     VTQQSNITTIQNDILIIRQWIDNYKEPNKLTITSGYASAANPGETVNIDTAFETFEAR
                     YCDYISVLGSTTKWTTAMSTMCPMLATEPQMQNLDLDMKDLANWIPNPNTVAENYNNL
                     WLTVCDLRTALQTLLESKAILPCVLISPNSVTIDSYNTISANISWTISSLTNIQSPIG
                     FSLEIWEWNGTTEGNLVYSNTYGDDVFTANVISTSIIAGREYIVKIKALYSCGESSAI
                     TVIGYLKEPIILYNINISESSDVGVTTSCDEGAGPSDYPYITTTTTYDLVNANTGLPA
                     TLATDLTIVVKHTITHPDYGVFTSNVPVTIPAGSSTVDYKYVSKQTILSSSGTCESVT
                     RVLTCGISINNSNCAFGNGIIEC"
BASE COUNT         1666 a          613 c          827 g         1558 t
ORIGIN      
        1 gtatttagca caaggaagtt ttcacacaac tgataaactt ggttctagct ttcatggtgg
       61 ttatcaagaa acagtaaaaa caaaaggtat taatcctaag tatgtaagta aattctatgt
      121 aacagaacct ggaacagctc ttaatgatgt atggactgtt tgtgcagaaa aaaattgtgg
      181 tttagaatgt gaaactacat acagattgag agttgatatt aaaggatctc cagttcttag
      241 aactttaacc agaaatatgt attttacagt agatgcttat actggttgtt gtgatgatcc
      301 tgataatcct tctgctgtag atcctaacgt tgttctttta caatgggcag atagaattaa
      361 tgaatatcct ttgacttctc cttttgtaaa ggcttttgta atgaataatc ctgttgaagt
      421 tgaagttgct tatgcagaag ctagtactct tgttactgat gatgctactg tagttcctgt
      481 tgcaggacaa gttgttgtag gggaaggtat tcccgtagga gcttatgtag gtactgtaac
      541 accagttggc ggatcgccta ttacagctta taaatttaca gtagttgatg ttgatggtaa
      601 tcttttagag attgcaaatg attatgataa ttctgtagaa ctatacacaa ttattgattc
      661 agaaacttat gttcctgcaa ctacttctgc agatgttgct aatattgttt cttgtcttgt
      721 agtaacaggt gcttatgttg atactacttt tggcgattgt tcatttagcc cttgtgacaa
      781 atatgaaatt gaaccagttc atattaattt atcagttgta gatgaaacag gtgatccttg
      841 tgttgtatca tgtttaacag ttactgaaag acaaaatgct tatcaaggta aaggatatgg
      901 agaacctctt ttaagagaat acatcttatc taaacgttat cagcaagaac cttggtttga
      961 tgatgtaaga cttcgtgaga ttaatgataa tactactatg actgatatta ataggtctgg
     1021 taaatattat gtatatcata ttcttcactc tgtacctcgt aaatcaaatt cagatggtat
     1081 gttagacaac gatcaatatc ttgtaaagat tgtagttaaa gctagagatg ctgattttga
     1141 agcttggatg aatgcttatt tggctagtgc taataatgga gtacaacttg aagttgttat
     1201 ctaagaaaaa aaatatttaa agaaaaggga gaaattaatc ttcctttttt ttgttttata
     1261 taaaaatttt tactatcttt gtattataaa tgataattgt atattatggc tattaagcat
     1321 agattagcat tagaaatatt agatacagca tgtgaccatg ctttaaagat ttctgatatg
     1381 tcttcctatg gacaaggttt acctgtagaa tgtcctagat tagatataac attgcctggt
     1441 ttttatcaac ctgtttattt aaataatctt acaccaggat ttaatgagat tataacaatg
     1501 ataacattag gttatcaaga agctaatgct acagaattaa atggttttcc agatggtttg
     1561 tacaccatta gatatagtgt ttctcctaat gataaagtat ttgttattta taatcattta
     1621 agaactactt gtataactaa tgaatattat agagaattgt gtaaattaca attagctgaa
     1681 tgtgaaccat ctacagaaat aaaacaaaaa ttaaatgatt taagatatat aaaaatgctg
     1741 attgatgctg ctaaggctaa agcagaatat tgtgacagtt gtgtacaagc tacagatatg
     1801 ttgggatatg ctcaaagaat gcttagtaaa tatcaatcag gtaattgttt aacttgtaat
     1861 taaaaaatta tggctagtac ttgtgtttgt ggtaataaat taggatgttc atgtcaaaga
     1921 cgtagagcat cagatggtac aatgagatgt acaaaatgta tagcagctta tgaagctaaa
     1981 ttaaaacaac taaaattaaa aaaatgattt atgattatat tgagaataca aatttagaaa
     2041 aaatatcaga actgtttgtt catagtattc aagaagatta tatcaaaaga agatttggta
     2101 ttaaatcttg taaaaatatt attgattata attatgctta tgatatttta aatctatata
     2161 aaagagcatt agaaagagat gcttgtaata tatgtgatga atcttattgt tgtacaaatc
     2221 aaattgaaga aagaataaaa acattataat atgagcaatt gtgttcctac atcatctaat
     2281 tgtattatct ggcaaggtcc taatattgaa tgtattgggt tatgtcaagg agatactatt
     2341 acagatgtta tatataaatt ggcaacagca tattgtgaat tatcaaacca attacaggct
     2401 gataattttg atatatcctg tatagaatgt ccagaaggga tagattgtac tgttgataat
     2461 attcaagatt tacttgatat attaattgct aaaatttgcg aattacaaac ttccgttgga
     2521 actcctggtg aacaaggaat acaaggacgt tctggtccac aaggacctcc tggtccacaa
     2581 ggtccagcag gtccacaagg tccacaaggt ccacaaggag aacctggtgt tacaggtgct
     2641 actggtccac aaggtccaca aggtccacaa ggtccaattg gtcctcaagg tcctagaggg
     2701 gaaagaggtc cagcaggtcc acagggagaa caaggaccgc aaggagaacc tggtgaatgt
     2761 ccttgttgta attttagagt aaaaattaca tggtatgatc ctggtcctta tgatgctaga
     2821 tatgtaggta ctgtatatgg tggaacacct ccatatacat atatgtggag attaaaagaa
     2881 ggtcctattt ccaatttaac actggtatat ccagattcaa taccatcaag atgtggaatt
     2941 tatcaattaa atcccaaaaa tggaatagga cttatagagg tagaagtaac agatgcaaat
     3001 ggttgtaaag cttttgatac aatattatat attaacttgt ctaatataat ttaaaatggc
     3061 attatccaaa aattgtacac cagtatcatc atcttgtgtt atttggaatg gtccaaaaat
     3121 agaatgtata gatcaatgtc aaaatgattc tatagaagat attgtatata gattaggaca
     3181 aataatatgt tctttatctg aaatgtctca aagtgattgt aattgtgata ataaaataga
     3241 taatctttta tataattgtc ttattactga tcaacaaatt ccacaatggg gagaaggggg
     3301 aaaaggatgg acagggcaac aattattaga aatatatgct gaagaattat gttcaagagc
     3361 aaacggtaat gttactgttc aagaccttgt aactcttcac aaaaaaataa atcaaccatt
     3421 agctttaaaa gatcatttac aatatgaaga agatggacaa actattacag aacttacata
     3481 cgatcaatat attttattat tagcacaatg ggtttgtgat ttatatgata ctcttgttac
     3541 acaacaaagt aatataacta caatacaaaa tgacatttta ataattcgac aatggattga
     3601 taattataaa gagcctaata aattaactat aacttcaggt tatgcttcag cagctaatcc
     3661 tggagaaaca gtgaatatag atactgcttt tgaaacattt gaagctcgtt actgtgatta
     3721 tatttctgta ttaggtagta ctacaaaatg gactaccgca atgtccacca tgtgtccaat
     3781 gttagctaca gaacctcaaa tgcagaattt agatttagat atgaaagatt tagctaattg
     3841 gataccaaat ccaaatacag tagctgaaaa ttataataat ttatggttaa ctgtttgtga
     3901 tttaagaaca gcattacaaa cattattaga atctaaggca atactaccat gtgtgttaat
     3961 atcgcctaat tctgtaacta tagattctta taatactata agtgctaata tcagttggac
     4021 aatatcttct ttaactaata ttcaaagtcc aatagggttt tcattagaaa tatgggaatg
     4081 gaatggtaca acagaaggta atttagttta ctcaaatact tacggagatg atgtatttac
     4141 agctaatgta ataagtacaa gtattatagc aggaagagaa tatatagtta aaataaaagc
     4201 tttatattct tgtggtgaat catctgcaat tactgttata ggatatttaa aagaaccaat
     4261 tatactttat aatataaaca tatctgaaag ttcagatgta ggtgttacta catcttgtga
     4321 tgaaggtgca ggtccttctg attatcctta tataacaact actacaactt atgatttagt
     4381 aaatgctaat acaggacttc ctgcaacatt ggctactgat ttaacaattg tagtaaaaca
     4441 tacaataaca catcctgatt atggtgtatt tacatctaat gtacctgtta ctataccagc
     4501 aggtagtagt acagtagatt ataaatatgt ttcaaaacaa actatattaa gtagtagtgg
     4561 tacttgtgaa tcagttacta gagtattgac ttgtggtata tcgatcaaca atagtaattg
     4621 tgcttttgga aatggaataa tagaatgtta aacaattaaa aatt
//
LOCUS       DAOSAB010000023         4632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_12588_length_4632_cov_3.065764, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000023 DAOSAB010000000
VERSION     DAOSAB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12588_length_4632_cov_3.065764"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK432_00850"
     CDS             <1..201
                     /locus_tag="PK432_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59509.1"
                     /translation="VFPKFIIYGCAVLVAHVLQRQFFPDFPAMKIIAGFVAYSELMSI
                     DENIQKITGFSVFKFFIKKLKK"
     gene            205..666
                     /locus_tag="PK432_00855"
     CDS             205..666
                     /locus_tag="PK432_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOK59510.1"
                     /translation="MTSANIIKTYGQPGDTKNLTMINLPFPMKIAWDTKVTVNKMQIH
                     KDAAASLTNIFKEILVVYGLPEIQRLGIDLFGGSYNFRQMRGGSEWSVHSWALAIDLD
                     PARNGLKATDKTAQFAKPEYKKMIDIFYKHGWYSLGREKNYDWMHFQFVKP"
     gene            671..976
                     /locus_tag="PK432_00860"
     CDS             671..976
                     /locus_tag="PK432_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59511.1"
                     /translation="MINWIKKTFPDTYEFIRNFFKALNNKSEGHSLRKWLAIGFFWLT
                     SVVVLRFTDATNLVMVIGVLCGMITTLIVTYSVSNYKEKQIKNKPEDSITDAINPQG"
     gene            1207..1614
                     /locus_tag="PK432_00865"
     CDS             1207..1614
                     /locus_tag="PK432_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59512.1"
                     /translation="MKQQSLYLTLLLLLSWTVSYAQHGASRDTSRCYGITELKKIAKT
                     AVDLHTCDTLLLTSKKMLANRDTLIKEKDYTITQYSLTIGIKDQMIKNREDTIAVKNQ
                     KIKKLTNQKKAIIVGWSATAIALTITTILALLH"
     gene            1665..1973
                     /locus_tag="PK432_00870"
     CDS             1665..1973
                     /locus_tag="PK432_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59513.1"
                     /translation="MFKFKPTIMSEEKKELTSEQVEENRNKVIQYYKKQVEVLEHQAK
                     YETLMADIEQARARRMEMIIRQAQMAAGPEDENNNPDNTDTPAEEATQEKKERKLKAK
                     "
     gene            1980..2381
                     /locus_tag="PK432_00875"
     CDS             1980..2381
                     /locus_tag="PK432_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59514.1"
                     /translation="MAKFNVVDKKVNMSLEDIVKFQIMTYCYIHKIILSESDLSCLTL
                     LGLNKKVELSDFCNACCDPSNRDKDVTISYQKAIFKTPQTVRNCVAKMRNYNIITRDT
                     KTVELNPELNIQHAGNILLNFKMFHVGTQES"
     gene            2362..2778
                     /locus_tag="PK432_00880"
     CDS             2362..2778
                     /locus_tag="PK432_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOK59515.1"
                     /translation="MVPKKANDFKKSVAEELGLSEDLVSNFIDFFWDRVRTHMSELNH
                     ETLQIPNLGTFKVKHWKIDETVESHKATIIRVEGKFAGYRMKIDLTDRIEKLERIKNL
                     VEQRELKFKEIRDARKNKNNMEEQGPDMGGADKQDL"
     gene            2986..3276
                     /locus_tag="PK432_00885"
     CDS             2986..3276
                     /locus_tag="PK432_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59516.1"
                     /translation="MNATSWLVQHEKDLAARIAKEAEYQLMWGHSPADQKRRIALEVT
                     QDLWRQAKITLEESINIAKMINSKALEDLDFAITLIETIKTKPYELSKMPNM"
     gene            3248..3601
                     /locus_tag="PK432_00890"
     CDS             3248..3601
                     /locus_tag="PK432_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59517.1"
                     /translation="MSFQKCPICNGTGDNPFVLELPRSTWPCPTCKGTRIISELTGLP
                     PVIEEKRNIFEPVDPCNADIQNAFVCNPEYRRADLKATSDAFIVYAKTIPTQTTTQDV
                     SNISSSESQIPESRP"
     gene            3642..4367
                     /locus_tag="PK432_00895"
     CDS             3642..4367
                     /locus_tag="PK432_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59518.1"
                     /translation="MFKQPFDPVAASKKASKNKNSKWYGIDPEEIQRIWSSETDRAVD
                     AGSLYHDQRESDITSIDTIQRSGVNIPIIKPIWDNGVKHAPNQRLTEGIYPEHFVYLK
                     SAGVCGQSDRVEVVRGIVDIIDYKTNKEIKKNSFVNWEGKAQMMTGPLSHVEDCNFNH
                     YALQLSTYMYIILKHNPQYKVGKQMLHHVIFEKEGDDPFGYPIIKKDPTGNPIVKTVV
                     PYEVPYMKSEVIAMIDHIKSIKK"
BASE COUNT         1701 a          737 c          875 g         1319 t
ORIGIN      
        1 gtatttccca agttcattat ttacgggtgt gctgtactgg tagctcatgt cttacaaaga
       61 cagttcttcc cagactttcc agccatgaag atcatagcag ggtttgtggc ttacagtgag
      121 cttatgtcta tagacgagaa cattcagaag ataactggtt tcagtgtatt caaattcttt
      181 atcaagaaac ttaaaaaata atatatgact tcagcaaaca ttatcaagac ctacgggcaa
      241 cccggagata ctaagaatct cactatgatt aaccttcctt ttcctatgaa aattgcatgg
      301 gatactaagg tgactgtaaa taaaatgcag atccataaag atgctgcagc atcacttact
      361 aacatcttta aagagatctt agttgtatat gggttaccag agattcaaag attaggtatt
      421 gacttgtttg gaggatctta taacttccgt caaatgagag gaggatctga gtggagcgtt
      481 catagttggg ccctggcaat tgaccttgat cctgctagaa atggtttaaa ggctactgac
      541 aagactgctc agtttgctaa accagaatac aagaagatga ttgacatttt ctacaaacac
      601 ggatggtact ctttaggtag agaaaagaac tatgactgga tgcattttca atttgtaaaa
      661 ccttaatatt atgattaatt ggattaaaaa aacatttcct gatacatatg agtttatcag
      721 aaatttcttc aaagctctta ataacaagag tgaggggcac tccttaagaa agtggttagc
      781 cataggattc ttttggttaa cctctgtagt agtactgaga tttacagatg ctactaatct
      841 agttatggta attggtgtac tttgcggtat gattactacc ctgattgtaa cctacagtgt
      901 aagtaattac aaggaaaagc agatcaagaa taaaccagaa gatagtatta cggatgccat
      961 caacccgcaa ggataataaa ttaactatac cttttatagc agcttgtaca ataatacttg
     1021 tattaatagt atttattata ttcagacctg cggctactgt tattagctct gacaaagaaa
     1081 agcagctaag agactccttg gttttactag agtctaatat aaacacaagc cttgctagac
     1141 aacatcaatt acaaaatcag tatgatagtc tgagtgcaat aggtcccggt ataatttata
     1201 gaaatcatga aacaacaaag tttatattta acactgcttc tcctactcag ctggacagta
     1261 tcatacgcgc agcatggagc atcaagggat actagccggt gctatggtat cacagaatta
     1321 aagaaaatag ccaagacagc tgttgaccta cacacctgtg atacattact cctaacatct
     1381 aaaaagatgc tggccaacag ggatactctt attaaggaga aggattatac tattacccaa
     1441 tattctctta caataggcat caaagatcag atgatcaaga accgagagga caccattgct
     1501 gtaaaaaatc aaaaaattaa aaagcttaca aatcaaaaaa aggctattat tgtaggctgg
     1561 tcagccacag caatagcttt aactattacc actatcttag ctcttctgca ttaatttaaa
     1621 cttttaatgt ataaatttgg taagtttaaa ctttatatgt atatttgttt aaatttaaac
     1681 caacaattat gtcagaagaa aagaaagagc tcacttccga gcaagtagaa gaaaaccgta
     1741 acaaggttat tcagtattac aaaaagcaag tcgaagtcct agaacatcaa gccaagtatg
     1801 aaactcttat ggctgatatt gaacaggcgc gtgctaggcg tatggagatg atcatccgtc
     1861 aagctcaaat ggcagcaggt ccagaggatg aaaacaataa tcctgataac acggatactc
     1921 ctgcagaaga agccacccaa gaaaagaaag aaagaaaatt aaaagctaaa taaaccaaca
     1981 tggctaagtt taatgtagtg gataaaaaag taaatatgtc cctggaagat attgttaagt
     2041 tccagataat gacctattgc tatatccata agataattct aagtgagtca gatcttagtt
     2101 gtcttacttt acttggtctt aataaaaagg ttgagctttc cgatttttgc aatgcttgtt
     2161 gtgatcctag taaccgggat aaggacgtga ctatctccta ccaaaaggca atatttaaaa
     2221 ctcctcaaac agtaagaaac tgtgtagcta agatgagaaa ttataatatt atcactaggg
     2281 atactaaaac tgttgaactt aatcctgaac tgaacattca acatgcaggt aatatattgt
     2341 tgaactttaa aatgtttcac gttggtaccc aagaaagcta atgattttaa gaaatctgta
     2401 gctgaagagc ttggattatc tgaggattta gtaagtaatt ttatagactt tttctgggat
     2461 agagtaagaa ctcatatgtc agaattaaat catgagacct tgcaaattcc aaacttagga
     2521 acctttaaag taaaacactg gaagatagat gaaacagtag aaagccacaa ggcaactatt
     2581 attagagtgg aagggaagtt tgcgggatac agaatgaaaa tagatctaac agaccggata
     2641 gagaaacttg agagaattaa gaacttagta gagcaaagag aattaaaatt taaagaaata
     2701 agagatgcta gaaaaaataa aaacaatatg gaagaacaag gccctgatat gggaggggct
     2761 gataaacaag atctttaaaa gagatcctgt tgaaaaagta tacaatgagc ggataaaaat
     2821 ctgcaaagaa tgtcctaatt tagataatac aggaaccaag tgtatggtag tgggtactca
     2881 accttgctgt ggtatttgcg gatgtagctt atcaatgaaa ttaagatcaa tggattcaca
     2941 atgcccgcat ccggatggac caaagtggga tatagctgat atgtaatgaa tgcaacaagc
     3001 tggttagttc aacatgagaa agatctcgcg gctagaatag ccaaggaggc agaatatcaa
     3061 ctaatgtggg gtcattctcc agctgatcaa aaaagaagga tagctcttga agttactcaa
     3121 gatctatggc gccaggctaa gattacttta gaagaaagta ttaatatagc aaagatgata
     3181 aactctaaag ctttagaaga tttggatttt gctatcacac ttatagaaac aataaaaact
     3241 aaaccttatg agctttcaaa aatgcccaat atgtaatggt acaggagata atccttttgt
     3301 actagaatta cctagatcaa cttggccttg tccaacttgt aaaggaacaa gaataattag
     3361 tgaacttaca ggactacctc cagtaataga agaaaagcgt aatatctttg aacctgtaga
     3421 cccttgtaat gctgatattc aaaatgcttt tgtctgtaat cctgaatata gaagagcaga
     3481 cttaaaagca acatcagatg cttttatagt ttatgcaaaa acaataccaa ctcaaacaac
     3541 aactcaagat gtcagtaata tttcaagctc agaatcacaa ataccagagt ctagacccta
     3601 atgataggat agactggatt agtgttacaa aatttgtagg aatgttcaag caaccttttg
     3661 atccggttgc tgcttctaag aaagcatcca agaataagaa ctctaaatgg tatggtatag
     3721 atccagaaga gatacaaagg atctggtcta gtgaaacaga ccgtgcagta gatgcaggat
     3781 ctctttatca tgatcaaaga gaatctgata ttactagcat tgatactata caaagatccg
     3841 gagtaaatat tccaattata aaaccgattt gggataatgg tgtaaaacat gcccctaatc
     3901 agagactaac agaagggatt tatccagaac attttgtata tttgaagtca gcgggtgtat
     3961 gtggacaatc tgatagagtt gaagtagtaa gaggtatagt agacattatt gattacaaaa
     4021 ctaataaaga gatcaagaaa aatagttttg taaattggga aggtaaagcc cagatgatga
     4081 ctggtcctct aagtcatgta gaagattgta actttaatca ttacgcttta caattaagta
     4141 cttatatgta tattattctg aaacataatc ctcaatataa agttggaaag cagatgcttc
     4201 accatgttat atttgagaaa gaaggtgatg atccttttgg ctaccctatt attaaaaaag
     4261 atcctaccgg taatcctatt gtgaagactg ttgttcctta cgaggtgcct tatatgaaat
     4321 cagaagtaat agctatgatt gaccatatta aatcaattaa aaaatgagaa tacctaaaat
     4381 aaaaataaag gtcagtctta tcccgggaat atgctttgga ataggaattc ctttatctga
     4441 ttatcaagac ttctacatat gttttttatg ttggggtata acctttaaat ggagaaaaag
     4501 atgagtaatg aagaatatac acaccagaac ttctggaaaa aagaaactaa gtttattact
     4561 ataatagata gagatcttaa gatacacttt acacctcttc ctgtatttga tgaatctttt
     4621 tgtaaacaag ta
//
LOCUS       DAOSAB010000024         4584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_12753_length_4584_cov_3.286156, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000024 DAOSAB010000000
VERSION     DAOSAB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12753_length_4584_cov_3.286156"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PK432_00900"
     CDS             <1..1079
                     /locus_tag="PK432_00900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK59519.1"
                     /translation="FQLPTIPDSREKIANIFKKEDTQTKKEIDNSDICKISVLKNEET
                     KIKEWNCNINIEVSTIKYIDWDKYYSLEIQGKYPKSIKAQVQIYECKKFSITDINTWN
                     KCKEVLTDTYTGDINLIYFGKIYANNKAGTLTNFNFTDSEYQTRSTFVDDISKQDVKV
                     YFSIYSQFKSKEWIDITYTTQKSITIPTAVKAAAVKPFSFPLSKLIGVTQWHGCTQYQ
                     CPHKGIDFGARLHNVIAVGDGTVADIGFDKYGGECNQGGRYIIIKHTNGMYSAYIHLN
                     SFLVKKGDSVKKGQIIGISGNSGMKNCQPLGYHLHFETRKGLSSSTHTDPVKYVNVDW
                     NLIPTLQYKQFPGRLTGDNPHPNF"
     gene            1089..1649
                     /locus_tag="PK432_00905"
     CDS             1089..1649
                     /locus_tag="PK432_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59520.1"
                     /translation="MFKEILFYILSLIFPSQKEQYTREYMINTKYLPVEVFWIDNTNV
                     LLSSYGYTQLFNTENRDSSVIDTCEDCIYGYDKNFVYCKYINRDIKSKNEFSTTIEIF
                     DINNQLLYSKDIFQTVAPVKCTRDSVLLHTNDPALEQHSYILDIKKDSLKEIKENIKD
                     TKEWFNNDKTKKIIIGEDKRVWLFKS"
     gene            complement(1635..3881)
                     /locus_tag="PK432_00910"
     CDS             complement(1635..3881)
                     /locus_tag="PK432_00910"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK59521.1"
                     /translation="MESYVYALIFLSLVNIIFGIYTLKESRVTTYIKYFAGSLFCVGF
                     WGIGLGGFLAAESPEVAMLWAKFHYVASIFVAYYLLVFALSLAKEEPIKLFYRILLIL
                     PIIAISVITVCCNNLLLKQAVIEEGGNVMILYKPGHIAYAIVFASYFIATLVVVLNKL
                     LKSSGIIQKQMILIFVGLLISGVLGMVYNLILPLLGNYKLVWVGPQFTVVFLALMFIG
                     MVRYQLFNIRLLAGKILFTGLTSVIMFLAFLFFTNVNKIVFGESVINLEFILFGLFCA
                     FLYSTFYESFRDFVLNKIQSKIITPGLDTSLVLKEYNRKLGKLSAKNEILNEFAQTLK
                     KALSPEYISIITKEGNEVINKQYVEYRDINYELIFETIEKEEDLKDMKYLAPPTLMSI
                     LQDENSSAVLSLEEFINELQTKGLVLLLPIVYEHDLLAVCVLGEGNSSSSTYIDEEIE
                     FLESLSDTTAVYLTRAILFEQLSEFNSVLQQKVGEQTKELQIKVRELEEARRKENDMI
                     DIMGHELRTPATIVKLNAEMLRKYIDSNPEEYAKYLDRISKAIDTEIGLINTLLTSAK
                     LEGNKVEIRKEEVDIKESIEMSVHGNEKDLKEGVKIYTDIQEGIPFAYADKIRTMEVL
                     DNLISNAVKYTDKGRIDVKARFDEKNIIVSVSDTGKGISKEDIPKLGSKFYRANNYLG
                     SKIVRPGGTGLGLYVTFRLVKLMGGKINVESEEGKGSTFTFTLPRYTNQKLDNTDTMD
                     KFTKLGLK"
     gene            complement(3903..>4584)
                     /locus_tag="PK432_00915"
     CDS             complement(3903..>4584)
                     /locus_tag="PK432_00915"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK59522.1"
                     /translation="SSGLSNGTMDIIYRINVPPKVNGLLDNLDIAVGCLQRRVVISNV
                     IAKNIADNEVQRIVSIAGGSCIIPIEAIYQTGKYGMQIVSVDYSPKANEKAEAIKKAG
                     EEYKDLGVGIKYLSSDLINEGIPSEIESSKSQLFECTGFWEYLNPDDRKKFLDRMSEA
                     MKDSDTFVLTALVDNPQQALFDKMGFKQLHPQETEEFIKQIEEAGLHVKEVYLTPNKA
                     YATAIVSR"
BASE COUNT         1621 a          865 c          619 g         1479 t
ORIGIN      
        1 aatttcaact tcccactatt cctgattcca gagagaaaat agctaacata tttaaaaaag
       61 aagatacaca aacaaagaaa gaaatagata acagcgatat atgtaagata tcagtactta
      121 aaaatgaaga aacaaagata aaagaatgga actgtaatat taatatagag gtgtcaacta
      181 ttaaatacat tgattgggat aaatactact cattagaaat acaaggtaaa taccctaaaa
      241 gtattaaagc acaagtacag atatatgaat gtaagaagtt tagtattaca gacattaata
      301 catggaataa atgcaaagag gtactcactg atacatatac aggagatatt aatttaatat
      361 actttgggaa aatatatgct aataacaagg caggaactct tactaatttt aattttacag
      421 attcagaata tcagacaaga agcacttttg tagacgacat atcaaagcag gatgtaaaag
      481 tatattttag tatttattct caatttaaaa gcaaagagtg gattgatatt acatatacaa
      541 cacaaaaaag tatcactata ccaactgcag ttaaagctgc tgctgtaaaa cctttttcct
      601 ttccactaag taaacttata ggggtcactc aatggcatgg atgcactcaa tatcagtgtc
      661 ctcacaaagg aattgatttt ggggctaggt tacataatgt aattgcagtt ggagatggta
      721 ctgttgcaga cattggtttt gataaatatg gaggagagtg caaccaagga ggaagataca
      781 taattataaa gcatacaaat ggtatgtact ctgcatatat tcacttaaat tcttttttag
      841 tgaaaaaggg agatagtgta aagaaaggtc aaattattgg tataagcgga aacagcggaa
      901 tgaaaaactg ccaaccattg ggatatcatc tacattttga aacaagaaaa gggctctctt
      961 catcaacaca tacagatcct gttaaatatg ttaatgtcga ttggaactta atacctactc
     1021 ttcagtacaa acagtttcca ggcagactta caggagataa tccccatcct aatttttaaa
     1081 gaaaatatat gtttaaagaa attctttttt acattctctc ccttatattt ccttctcaaa
     1141 aagaacagta tacaagggaa tatatgataa acacaaagta tctcccagta gaagtttttt
     1201 ggattgataa tactaatgta cttcttagct catacggcta tacccagtta tttaatacag
     1261 agaatagaga tagtagtgtt atagatacat gtgaggattg tatatatgga tatgataaaa
     1321 actttgtata ctgcaaatat ataaatagag atattaaaag caaaaacgaa tttagtacaa
     1381 ctattgaaat ttttgatatc aataatcagc ttctttacag taaagatata tttcagactg
     1441 ttgctccagt aaaatgtaca agagattctg ttttacttca tactaatgat cctgctctag
     1501 aacagcacag ctatatccta gacatcaaaa aagatagttt aaaagagata aaagagaata
     1561 ttaaagatac aaaagaatgg tttaacaatg ataaaactaa aaagattata attggagaag
     1621 ataaaagagt atggctattt aagtcctaat ttagtaaatt tgtccattgt atctgtattg
     1681 tctagcttct gattggtata tcttggtagt gtaaatgtaa atgtactacc cttaccctct
     1741 tcactctcta catttatctt tccacccatt aatttcacta atctaaaagt tacatacaaa
     1801 cctaaacctg ttcctcctgg tcttactatc ttagaaccta aataattatt cgccctgtaa
     1861 aacttactac ctaatttggg tatatcttcc ttagatatcc ctttgcctgt atcacttaca
     1921 ctcactatta tgttcttctc atcaaatcta gcctttacat ctattctacc tttatctgta
     1981 tactttactg cattacttat taaattatct aatacctcca ttgttcttat cttatccgca
     2041 tatgcaaaag gtataccctc ctgtatatct gtatatatct ttactccttc ctttaaatcc
     2101 ttctcgttac catgtactga catctctata ctctccttta tatccacttc ctctttcctt
     2161 atctccacct tattcccttc taacttagct gaggtaagta atgtatttat caaccctatc
     2221 tctgtatcta ttgccttact tattctatcc aaatacttcg catactcctc aggattacta
     2281 tctatatact tccttaacat ctctgcattc aactttacta tcgttgctgg tgtccttaat
     2341 tcatgcccca ttatatctat catgtcattc tccttccttc ttgcttcttc taactccctc
     2401 acctttatct gtaactcctt tgtctgttca cctaccttct gctgaagcac agaattaaat
     2461 tccgacaatt gctcaaacaa aatagcacga gttaaataca cggcagttgt atcagataat
     2521 gactctagaa attctatctc ctcatctata taagttgaac tagaagaatt tccttcccca
     2581 agaacgcaca ctgctaaaag gtcatgttcg tacactatag gtagaagtag tactaatccc
     2641 ttagtttgca actcattaat aaattcttca agagagagaa ctgctgacga attctcatcc
     2701 tgaagaatac tcatcaaggt tggaggagca agatatttca tatcttttaa gtcctcttct
     2761 ttctctattg tttcaaaaat tagttcataa ttaatatctc gatactcaac atactgctta
     2821 tttataactt cattaccctc ttttgtaatt attgatatat actctggaga aagtgctttc
     2881 ttaagagttt gagcaaactc attaagaatt tcattcttag cagataactt acccaacttt
     2941 ctattgtact ctttcaatac caatgaagta tccaaaccag gagtaataat tttagattga
     3001 attttattta atacaaaatc tctgaaactt tcataaaagg ttgaatacaa gaaagcacag
     3061 aacaaaccaa aaaggataaa ttctaaatta attacactct ccccaaaaac aatcttattt
     3121 acatttgtaa aaaacaaaaa cgccaaaaac ataatgactg atgtgagtcc cgtaaaaaga
     3181 atctttccag caaggagtct gatattaaat agttgatacc taaccatacc aatgaacata
     3241 agagctaaga aaacaacagt aaactgagga ccaacccaga ccagcttata atttcctaaa
     3301 aggggaagga taagattata taccattcca agaacacctg aaatgaggag accaacaaaa
     3361 atcaaaatca tctgcttctg aataatacct gacgatttta acagcttatt cagcactaca
     3421 actaaagttg ctataaaata tgaagcgaac acaattgcat atgctatatg tcctggtttg
     3481 taaagtatca tcacattccc accctcttct ataactgcct gtttaagaag taaattatta
     3541 cagcatactg taattaccga tatggcaata attggcaata tgagtaaaat tctgtaaaaa
     3601 agtttaattg gttcctcttt tgctaaggat agtgcaaata ctagcaaata atatgcaacg
     3661 aaaatagatg caacataatg aaactttgcc catagcattg caacctcagg tgactcagca
     3721 gctaaaaaac ctccaagtcc tataccccag aagccaacac aaaacaaact acctgcaaaa
     3781 tatttaatat aagttgtaac tcttgattcc tttagtgtat atataccaaa gatgatatta
     3841 acaagagata gaaatattaa tgcatataca taactttcca ttataggcag tttttaaatt
     3901 ttttaacgac taacaattgc agtagcatat gccttattag gcgtaagata tacctctttt
     3961 acatgtaaac cagcttcctc tatttgtttt ataaattcct ctgtttcctg aggatgcaac
     4021 tgcttaaatc ccattttatc aaagagtgcc tgctgagggt tatctactag tgctgtcaaa
     4081 acaaaggtat cagaatcttt catagcttct gacatacgat ccaagaattt tttcctatca
     4141 tctggattaa gatactccca aaagccagta cattcaaaaa gctgtgactt agaactctct
     4201 atttctgagg ggatcccctc atttatcaaa tctgaagaca gatattttat tccaacacct
     4261 aaatctttat actcttctcc agccttttta atggcctctg ccttttcatt tgccttaggg
     4321 gaataatcta cacttactat ctgcatacca tattttccag tctgataaat agcctcaata
     4381 ggaattatac aacttccacc tgctatagaa actattctct gaacttcatt atccgctata
     4441 ttttttgcaa taacattgct aataacaacc cttctctgaa gacatcctac agcaatatca
     4501 agattatcta ataaaccgtt taccttagga ggaacattta ttctgtatat tatatccatt
     4561 gttccattcg ataaaccaga agaa
//
LOCUS       DAOSAB010000025         4386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_13417_length_4386_cov_3.859386, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000025 DAOSAB010000000
VERSION     DAOSAB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13417_length_4386_cov_3.859386"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /gene="argS"
                     /locus_tag="PK432_00920"
     CDS             complement(<1..1446)
                     /gene="argS"
                     /locus_tag="PK432_00920"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOK59523.1"
                     /translation="MDIQQEITDNLKDIVNSMGALGDTVKVEIPAQSSFGDMTSNIAM
                     QISKQLNANPVDVAKEIVRRLPKNENIEKIEVVSPGFINFFFSDKYLLEILKEVNINK
                     EYFNIDVLKGKKYVVEYTDPNPFKIFHIGHLYTNMVGESFARLTEALGALVIRANYQG
                     DVGLHVAKTMWGLGKMLKDDGITFNDLAKRPLNERVKYLGDAYMLGFKMYDDIKDKEV
                     IDDVRNINYYIFSLYIKSLPKREFFDELDKRQIKEMYFESRKWCLESFEEIYKRTGTK
                     FDKYYFESQMGEDAIKIVRENMNGKGKNIFKESEGSVIYEGDPKKGLHTRVFLNKEGL
                     PTYESKEIALALKKYEDFKFNKSIIITANEQTPYFNVVLDALSQLEPLIANSTVHFGH
                     GMVKLPGAEKMSSRKGKIIEGEWLLNEAKRKVEEIMRKSEKWSESEIADVSDKIAVAA
                     IKYSFLKVSVGKDVIFDLNKATSFDGDSGPYL"
     gene            complement(1467..1540)
                     /locus_tag="PK432_00925"
     tRNA            complement(1467..1540)
                     /locus_tag="PK432_00925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1504..1506),aa:Arg,seq:tcg)
     gene            complement(1544..1617)
                     /locus_tag="PK432_00930"
     tRNA            complement(1544..1617)
                     /locus_tag="PK432_00930"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1581..1583),aa:Arg,seq:gcg)
     gene            complement(1647..2465)
                     /locus_tag="PK432_00935"
     CDS             complement(1647..2465)
                     /locus_tag="PK432_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK59524.1"
                     /translation="MNSHLIQNLIWVFLSLIYLSCVRAENEVVFLNVGQGDSILVQYG
                     STQLLIDGGPDSSVLYEIQKYMPIYDRNIEYVLLTHPHDDHILGLLKVLDRYSVGEIL
                     YYPVCFENENYKYLLQRYDNKREVGRGDTIRLGYIEVNILWPILNKEESKNGCVESYN
                     GNINNDSLVIEFEYLGKSFLLMGDAEQEVEKVLVDKGFLQFGNSYDILKAGHHCSNTS
                     SSETFVKAVSPKLVICSAGINNSFGHPGSETLKTFQRLNVQYLLTYEKGDIRIK"
     gene            complement(2462..3976)
                     /locus_tag="PK432_00940"
     CDS             complement(2462..3976)
                     /locus_tag="PK432_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOK59525.1"
                     /translation="MSLKQFKQRITALLLFFIDYQALLYCFTFLVLSISLSNFLEKVI
                     FERIPVSIGLIARIFLPAFFLFIFFLVSYYLFCHVHIFATGLKILLLSTLLFLPIFIF
                     QYLKISKAVQKVQSFTGQNVSVTGILMSQEKESTYIIFPKGGQVGELIIKFDHTPVLH
                     AGQECKISGEVVQPESFEDFDYRRYLFRKGIYAILQVKEYECSNGGNIFFEARYSLER
                     IVEKSVPEPEASLLIGIMFGSKRIFLSEFNTALNSSGVSHVIAASGYNVALVAQGVDL
                     LFKKNRGKGVVLIKICCIWGFSMFSGLSSSLIRAATMSTLSLIANLFGRESNSGATVI
                     FCATLLILLNPFLIHDVGFLFSFASVIGLIFFPKCFENIKSSFLKDSILPSLTCILFT
                     IPISIAFFGKVSVISLLSNIVIVPIISSSIFWGLGATVINIFLPLQILYLIPYIQLTV
                     FKNLVMLSSSVEMVELRINGGVFAVAVYLLLFLFCLYNYPIEPSNYYSLQSKKI"
     gene            complement(3967..>4386)
                     /locus_tag="PK432_00945"
     CDS             complement(3967..>4386)
                     /locus_tag="PK432_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOK59526.1"
                     /translation="IIDAVKRANGFTQDAGFKYISMKMNLSAEMKDNSKVYIPFESDY
                     NCNLLAFSLPKEVVDIINAPQESNDDVNNGDSTNDGGCISINNATLQELETLNGVGPS
                     TAQKIIDGRPYTKIEDLLNVSGIGEATLNKFKDSICL"
BASE COUNT         1511 a          885 c          557 g         1433 t
ORIGIN      
        1 aagatacgga ccactgtctc catcaaaact tgtagcttta tttaaatcaa agataacatc
       61 cttaccaaca gaaactttaa gaaaagaata tttaattgct gcaactgcaa ttttatcact
      121 aacatcagct atttcactct cactccactt ttcacttttt ctcattatct cctcaacctt
      181 tcttttcgcc tcatttagta accactcacc ttcaatgatc ttaccctttc tacttgacat
      241 tttctcagct ccaggaagtt taaccatacc atgaccaaag tgaacggtac tattagcaat
      301 taagggttct aactgcgaaa gtgcatctaa aactacatta aagtagggag tttgctcatt
      361 agcagttatt ataatagatt tattaaattt aaaatcttca tatttcttta atgccaatgc
      421 tatctccttt gactcatatg ttggaagccc ctctttattt aaaaacaccc ttgtgtgaag
      481 tcccttttta ggatctcctt catagataac tgaaccttca ctctctttaa aaatgttttt
      541 acctttccca ttcatatttt cccttactat ctttatagca tcttctccca tctggctttc
      601 aaaatagtac ttgtcaaact ttgtacctgt tcttttgtaa atctcttcaa aagactcaag
      661 acaccatttt ctactctcaa agtacatttc tttaatctgt cttttatcta actcatcaaa
      721 aaactctctt ttgggaaggg atttaatata caaggaaaat atatagtagt taatgtttct
      781 aacatcatca ataacttcct tatctttaat atcatcatac atcttaaaac caagcatata
      841 tgcatcacct aaatacttaa ccctttcatt tagtggtctt tttgctaaat cattaaatgt
      901 aataccatca tctttaagca tttttcccag tccccacatt gttttagcta catgaagacc
      961 aacatctccc tgataattag cacgaataac taaagctcct agcgcctctg taagccttgc
     1021 aaaagattca cctaccatat ttgtatacag atgcccaata tggaatatct taaaaggatt
     1081 aggatctgta tactcaacta catacttttt acccttaaga acatcaatat taaaatattc
     1141 tttgtttata tttacctctt taagaatctc caaaagatat ttatcagaaa aaaagaagtt
     1201 tataaatcca ggagatacta cctctatctt ctcaatattc tcattcttgg gaagtcttct
     1261 tacaatttcc tttgctacat caacaggatt agcattaagc tgctttgaga tttgcattgc
     1321 aatattagat gtcatatcac caaaagaaga ttgtgcagga atttcaactt taacagtatc
     1381 tcctaaagcc cccatactgt ttacaatatc tttaagattg tcagtaattt cctgttgtat
     1441 atccatagta tagtatatat caaaaatgca cccggaagga gttgaaccct caacactcgg
     1501 ttccgaagac cgatgctcta tccaattgag ctacgggtgc atatgtactt ggacagattt
     1561 gaactgtcaa cctttggttc cgcaaaccaa tgctctatcc aattgagcta caagtacacc
     1621 tcctcatata ttttatcaca ttccacctac tttatccgta tatccccttt ctcatatgtc
     1681 agtaaatatt gaacattaag cctttggaaa gttttcagtg tttcactccc aggatgtccg
     1741 aaagaattat ttatcccagc agaacatatt actaacttag gagatactgc ttttacgaag
     1801 gtttcactac tagatgtatt agaacaatga tgccctgctt ttaatatatc ataactatta
     1861 ccaaattgaa gaaaaccctt atctacaaga actttttcaa cctcctgctc tgcatcaccc
     1921 atgagaagaa aacttttacc caaatattca aattcaataa caagggagtc attatttata
     1981 ttcccattat atgattcaac acatccattc ttactttcct ctttattaag tataggccat
     2041 aatatattaa cctctatata tcccagtctt atagtatccc ctcttcccac ctctctttta
     2101 ttatcatatc tttgtaacaa atacttatag ttttcattct caaagcatac tgggtagtaa
     2161 agaatctccc caacactgta tctatccaga actttaagaa gccctagaat atgatcatca
     2221 tgaggatgag taagaagaac atattctata tttctatcat atattggcat atatttctga
     2281 atctcataca aaacagaaga gtctggtccg ccatctatta gcaattgtgt gctaccatac
     2341 tgaacaagaa ttgaatctcc ctgtcctaca tttaaaaata caacctcatt ttcagctctc
     2401 acgcaagaaa gatatatgag agagagaaaa acccaaatca aattttggat taaatgagaa
     2461 ttcatatctt tttagactgt aaactatagt aattggaagg ctctataggg tagttataca
     2521 aacaaaacag aaataataac aaatatactg caacagcaaa tactccccca ttaattcgta
     2581 gctctaccat ttctacacta gaagacagca ttacaagatt tttaaaaaca gttaattgaa
     2641 tatatggaat taaatataaa atctgtaaag gaaggaagat atttattact gttgcaccta
     2701 atccccaaaa tatactactg ctgattatag ggacaattac tatattagaa agaagagaaa
     2761 tcacagatac ttttccaaaa aatgcaattg aaatgggaat agtaaataaa atacaagtta
     2821 aagatgggag aatactatct tttaaaaatg aagacttaat attttcaaag cattttggga
     2881 agaagataag accaattaca gatgcaaaag aaaaaagaaa accaacatca tgtataagga
     2941 aagggtttaa aagtataaga agagtagcac aaaatattac agtagcacca ctattactct
     3001 ctctcccaaa tagattagct attaaagaaa gtgtactcat tgtagctgct ctaatcagtg
     3061 atgatgaaag acctgaaaac attgaaaatc cccatataca acagatcttt atcaatacta
     3121 caccctttcc tctgttcttc ttaaacagta aatctacccc ttgtgctact aaagccacat
     3181 tatacccaga tgcagcgata acatgagata caccgctact atttagtgct gtattaaact
     3241 cacttaaaaa tatccttttg cttccaaaca ttatcccaat taagagtgat gcttcaggtt
     3301 ctggaacaga cttttctaca attctttcaa gagaatatct tgcttcaaaa aaaatatttc
     3361 ctccattact acattcatac tctttaactt gcagtattgc atatattcct tttctaaaaa
     3421 gatatcttct ataatcaaaa tcttcaaaac tctcaggttg cacaacttct cctgagattt
     3481 tacactcctg accagcatgt aaaacaggtg tatggtcaaa tttaatgatt agttctccta
     3541 cttgtcctcc ttttggaaaa attatatatg tactctcctt ctcttgagac attaaaattc
     3601 cagttacaga cacattttgg cctgtgaaac tttggacttt ttgaactgct tttgaaattt
     3661 taagatattg gaagataaaa attggaagaa ataaaagagt ggataacaac aatattttta
     3721 gcccagttgc aaaaatatgt acatggcaaa acaggtagta tgaaacaagg aagaaaataa
     3781 aaagaaagaa agcaggaaga aagattctag caattaaacc aattgaaaca ggaattcttt
     3841 caaaaatcac cttttcaaga aaattagaga gcgaaattga cagtacaaga aatgtaaaac
     3901 agtataacaa tgcttgataa tcaatgaaaa acagtaaaag agcagtaatt ctttgtttaa
     3961 actgctttaa agacatatac tgtccttaaa cttgttaagt gtagcctcgc ctattccact
     4021 tacattaagc aaatcttcta ttttagtata tggtctgcca tctataatct tttgagcagt
     4081 ggagggccct actccattta aagtctctaa ttcctgcaaa gtagcattat ttatgcttat
     4141 acatccgcca tcattagtac tatctccatt attaacatca tcattgcttt cctgaggcgc
     4201 atttattata tctacaacct ccttagggag actaaaagct agcagattac agttataatc
     4261 gctctcaaaa ggtatataca ccttagaatt atccttcatc tctgctgata agttcatttt
     4321 cattgatatg tacttaaaac ctgcatcctg cgtaaaccca ttagcccttt taaccgcatc
     4381 aataat
//
LOCUS       DAOSAB010000026         4377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_13449_length_4377_cov_3.223739, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000026 DAOSAB010000000
VERSION     DAOSAB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13449_length_4377_cov_3.223739"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..2784)
                     /locus_tag="PK432_00950"
     CDS             complement(190..2784)
                     /locus_tag="PK432_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOK59527.1"
                     /translation="MSDTPIKVKRAVYFWEDYAFTKLQESSVGRKGLSLFELKDMDIP
                     IPDFFVVSPIVFDKVVSQAIVRDAQTLFEKGKNPEESEVLDSILKTELDEEYIEEILS
                     AYARISGFTDAWVSVRSSVVYPANPQVSFSGVFSTELNVRGTRNLIDSIKRVYASLFT
                     DDVVAYAASKGINLADVKLAVVVQKMVQAEVSGVAFTIDPITQDPTKLSIEAVFGLGD
                     TIALGELTPDIYLLNKKDLNILEKRISPQEWMKIRSVNRSSNKTEGTQKVTISNAWSH
                     RQKVDDRYLKEIAKIALIVENKLRRAQSIEWVMSGGKIWVLQSKDLYERYSPQDVVIE
                     SAKSFDTLGEVLRWIFEKYQGIGMLEDKAISNAQKMVQSSKHEYNPLTEKLISAAKGV
                     VRKEEKEQGRQTSDVGDLIVKGVGASFGIVSGNVKIIEKNSKAEVNKKDILVIKEYSS
                     DMEPLIINSGGVILETGGITSDVAILCREFDIPAVVGAVQASSLLKDGQLIRLDGYTG
                     SVYKEKTDEKVISPTHPVVQAYVQEALSGVNLLEKEEKKEEIVQPIPEEKSEEKKEYK
                     IPHDMNLAPCATKVFIRPLDTPENLVDYVGNSHGIVYIDMDRIMIEDGRHLLAYVEDK
                     KFVEYAKRISEKICEYVDLAEGNQVVLAIGAAPVSEFRNLVKGKQFENSEIGDLVHGL
                     PHYLSNKEFLNRVIRIVRRVRNVYKCRNVDFAVYSPTDVKSMTEFKKLLLSGGLRRGV
                     SFRVYAILDNPSDVILADDILTTKIDGLIVDMPRIAKIMQGADIYDMEAKYDLGANSS
                     LKVVDAICDISKVPSKELIVIADDSKELVRYSVQRGVYGVSVLPSSVKDMRKLVSEEE
                     AKIILSKK"
     gene            complement(2809..3942)
                     /locus_tag="PK432_00955"
     CDS             complement(2809..3942)
                     /locus_tag="PK432_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOK59528.1"
                     /translation="MKVLNINKPEEKRDYIKELSELQKARSQKQTQQPVFTIEFSWKF
                     LALVFFVMALVLWGEQLLGIAVFLFLSFVIMSAALPVINWFKKEGISKGWAIAVTYFL
                     GFVLLLAVVALIVIPLINQINGLGKNIPVWVENITENLKELDVSGFVINEALTQWFER
                     FTSADSFKNITSAIGGAAEWASIVLASFVFSVYLILDHDKVLDFVLIRIHSDEKRSRV
                     KKLVLDLEYKLGKWLIGQATVSAIAGTTLGLVLALFRIPFALPMGVLVALLSAIPSLG
                     ATIASIPPLLTALILKGPMTALIILLIFLVYQQVENNLIIPKVMGNAVEVKPTLILLA
                     AITFLILFGVWGAVLSVPAMVILKMCYEFYIDLQKIDAKGSID"
     gene            complement(3988..>4377)
                     /locus_tag="PK432_00960"
     CDS             complement(3988..>4377)
                     /locus_tag="PK432_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOK59529.1"
                     /translation="LMDIFFISILIGSMGVLDDVVMAQISAVFELYTTDEKLNGFDLY
                     RKAMNIGKDHLSSMVNTLFLAYAGSSLATVMLLIYNSKEMGNILQMDFIAEEIVRSLV
                     SSMGILLIVPITSIIAAKVIPSILNEK"
BASE COUNT         1443 a          946 c          526 g         1462 t
ORIGIN      
        1 ctctttaacc ctttcattaa cagcaatatc aaatccttca acttcaatac ccaagtagaa
       61 aagaaattta gaaatgtagg aaacatctcc accacctatg cctaggcaaa ctatcttatg
      121 gacattgtcc aaattcaaat tcataatcca tggattatac caaaaacaac agggaaatat
      181 taaagactat tactttttac ttagtattat ctttgcttct tcttctgaaa caagctttct
      241 catatcttta acagaggaag gaagtacaga taccccatac acacctcttt gtacactata
      301 tctaacaagc tccttactgt catctgctat cacaattaac tctttagaag gcaccttaga
      361 tatgtcacat attgcatcaa ctacctttaa tgagctgttg gcacctaaat catattttgc
      421 ctccatgtca tatatatctg caccctgcat tatcttagca attctaggca tatccactat
      481 taaaccatca atttttgtag ttaaaatatc atcagccaaa attacatcac taggattatc
      541 aagtattgca taaaccctaa aagatacccc ccttcttaaa ccaccagaaa gaagaagttt
      601 cttaaactca gtcattgatt taacatcagt tggagagtaa acagcaaagt ctacatttct
      661 acatttatac acatttctta cccttcttac aattctaata actctattta aaaactcttt
      721 gttagaaaga taatgaggaa gaccatgtac caagtctcca atttcactat tttcaaactg
      781 ctttccttta acaagattcc taaactcaga aacaggagca gcccctatag ctaagacaac
      841 ttgattacct tcagctaaat caacatattc acatattttc tctgaaatcc tttttgcata
      901 ttcaacaaac tttttatctt ctacatatgc aagtaaatgt cttccatctt ctatcataat
      961 cctatccata tcaatgtaaa caataccatg agagtttccg acataatcaa ctaagttctc
     1021 aggtgtatca agaggtctaa taaacacctt tgtagcacat ggagccaagt tcatatcatg
     1081 aggaatcttg tactctttct tctcttcact tttctcctct ggtattggct gaacaatttc
     1141 ttctttcttc tcttcctttt ctaaaaggtt aacacctgaa agtgcttcct gaacatatgc
     1201 ctgtacaaca ggatgagtag gggatattac tttttcatca gttttttctt tatatacact
     1261 ccctgtatat ccatcaagtc tgattaattg accatcttta agcaatgaac ttgcttgaac
     1321 tgcaccaaca actgctggga tatcaaattc acggcacagt attgctacat cacttgtaat
     1381 tccgccagtt tcaagaataa cacctccact attaattatt aaaggctcca tgtcagaaga
     1441 gtactctttt ataacaagaa tatccttttt gttaacctct gcttttgagt tcttctcaat
     1501 tatctttaca ttaccactta caatcccaaa acttgcaccg actcctttaa ctattaaatc
     1561 accaacatca ctagtctgcc tcccttgttc tttctcctct tttctaacta ctccttttgc
     1621 agcagaaatt aatttctctg ttaatgggtt atattcatgt ttactacttt gaaccatttt
     1681 ttgagcatta gaaattgctt tgtcttctaa cattcctatc ccttgatatt tttcaaatat
     1741 ccatcttaaa acctctccta atgtatcaaa actttttgca ctttcaatta caacatcctg
     1801 tggagagtat ctttcataca aatccttact ctgcaaaacc caaatctttc ctcccgacat
     1861 tacccattct atactctgtg cccttcttaa tttattttca actattaatg ctattttagc
     1921 aatttccttt aaatacctat catctacttt ctgcctatgg ctccatgcat ttgatattgt
     1981 aaccttctgt gtcccctctg ttttatttga acttctattt acactcctta tcttcatcca
     2041 ctcttgagga gatattctct tttctaaaat gtttaaatcc tttttattga gcaaatatat
     2101 atcaggagta agctcaccca gcgcaattgt atcacctaaa ccaaatactg cctctatact
     2161 taactttgtt ggatcctgtg ttattggatc tattgtaaat gcaacaccac tcacctctgc
     2221 ctgaaccatt ttttggacaa ctactgcaag tttaacatca gccaaattaa tacctttaga
     2281 ggcagcatat gctacaacat catcagtaaa caaagatgca tatactctct ttatggaatc
     2341 aatcaaattc cttgtacctc ttacatttaa ctctgttgaa aataccccag aaaaagatac
     2401 ttgagggtta gcgggatata caacggaaga ccttactgaa acccatgcat cagtaaatcc
     2461 agatattctt gcatatgcag aaagtatctc ctctatatat tcttcatcaa gttcagtttt
     2521 aagaatagag tcgagtactt ctgactcttc aggatttttt cctttctcaa ataatgtttg
     2581 tgcatctcta actattgctt gagatacaac tttgtcaaat acaattggtg atacaacaaa
     2641 aaaatcagga attggaatat ccatatcctt aagttcaaaa agtgataaac cctttcttcc
     2701 tacagatgat tcctgcaatt tcgtaaatgc ataatcttcc cagaagtata cagctctttt
     2761 aactttaatc ggtgtatcag acattcttta ggcagttaaa tatttatatt aatctatgct
     2821 acccttagca tcaatttttt gcaagtctat atagaactca tagcacatct taagtataac
     2881 catggcagga acagaaagaa ctgctcccca tactccaaat agtattaaga atgtaattgc
     2941 agctaaaagt ataagggtag gttttacctc aactgcattt cccataactt taggaattat
     3001 caaattattt tcaacctgct gatatactaa gaatatcaaa agaataatta atgcagtcat
     3061 aggccctttt aagattaatg ctgtaagaag aggcggtatt gaagctatag ttgcacccaa
     3121 tgaaggaatt gcagagagta gggcaactaa aacacccata gggagtgcaa aaggtatacg
     3181 gaacagtgca agtactaaac ctaatgttgt cccagcaatt gcacttaccg ttgcctgacc
     3241 aattaaccat tttcccaatt tatactctaa atccaaaaca agttttttaa ctctagacct
     3301 cttttcatct gaatgtatcc taattaatac aaaatccaac actttatcat gatctagtat
     3361 caaataaaca gaaaaaacaa aagatgctag tactatagag gcccattcag cagcaccacc
     3421 aatagcactt gtaatatttt taaaactatc agctgatgta aatctctcaa accattgtgt
     3481 aagtgcttca tttattacaa agccactaac atctaactcc tttaaatttt cagtaatatt
     3541 ttcaacccaa acaggaatat ttttccccaa tccattaatc tgattaatta gaggtataac
     3601 tattaaagca acaactgcaa gaagaagtac aaaacctaag aaatatgtaa ctgctattgc
     3661 ccatcctttt gatattccct cttttttaaa ccagtttata actggaagag cagcagacat
     3721 aattacaaag cttaaaaaaa gaaatactgc aatacccaaa agctgttccc cccataatac
     3781 taatgccata acaaaaaata ctaatgccaa aaacttccat gaaaattcaa tcgtaaagac
     3841 aggctgttgt gtttgttttt gacttctagc tttttgaagt tcacttaact ctttaatgta
     3901 gtctctcttt tcttctggtt tatttatatt taatactttc ataatttatt attaacatat
     3961 tactcagact aataactaaa gtatacatta tttctcattc aatatgctag gtattacctt
     4021 cgcagcaatt atagaagtaa ttggtactat taaaagtatt cccatacttg acactaaact
     4081 tcggacaatt tcctctgcaa taaaatccat ctgcaaaata tttcccattt ctttgctatt
     4141 gtagataaga agcattacag tagcaagact tgagccagca taagcaagaa aaagagtatt
     4201 caccattgat gataaatgat ctttcccaat attcattgcc tttctataca aatcaaatcc
     4261 atttaatttc tcgtctgtgg tatataactc aaaaactgct gaaatttgag ccattacaac
     4321 atcatccagc acacccatgg aaccaattaa aatgcttatg aaaaagatat ccatcaa
//
LOCUS       DAOSAB010000027         4119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_14520_length_4119_cov_15.041831, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000027 DAOSAB010000000
VERSION     DAOSAB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14520_length_4119_cov_15.041831"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4119
                     /locus_tag="PK432_00965"
     CDS             <1..>4119
                     /locus_tag="PK432_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59530.1"
                     /translation="LAQFGAAINQLAIGIIIGQTVQAIGTILAIPEIVSEDFKIDNPI
                     YKIGKGITEWAEKATPIYQTGERWSDFGHWAQGLVSIGSAASFLIPGYGIFKAVNYLG
                     KLLRLSEFTTLALATGIGALASRHMEGAMEADQNYRVSEEKLTNIWRTGSFDDDSDNS
                     ILANLYKQKSIDISNILNRGDLKKGDKIREIEKISNLYDNEASKLINDAANIGARTDY
                     LLNYANLIFDIVQFGALSKFWQKTSRRMAGAVERELIKAESKTLGMKAISEEALPKTR
                     LDRIISGMGRGVKAYFPETTEGLEEVWNEISAHEGERAAMIASGVKKDDNSTFGERVL
                     DYLGMDQTFDAFVFGALGGVAFQWLGGKIGFGADRDLLNKKIAETAARDRLLKETAQK
                     IKEIQTDEKLTADQKAEEIKHLAYKTGYQIGLQASIYNNMDRVDAQMKSPGLISDLEK
                     LEMGDKKSITETISTMHQAALEAEKNFNRYFSIVRNKNISHNAKIFFAQDAADLHSTI
                     VGIGKEINDYKTKLGEAIKQYEEISGKSLNDIEKSILELTGLNVAIDELNKYSTNTVN
                     SKNSREFSKNIIKKLEQRKLELEKQIGNTRVQGKDIGGVHIMTVLKYPNHLANIYTLE
                     AARELHSEKLQKLMTEEGQKEYEKQIKKEIEEERKKKTEERQAREYNKNKDKTKSENQ
                     QNTSQQSTETPSTSKSTNNETGETGEAEETGKAEEPEETSKKDLPNIVPGRPGVIPEE
                     EEKKKDDDSKSGGKETGHEVEPGEAGVSTEPYDLPDGRIEGTPYEVKDRTLYYNGQPV
                     GNPNKKELGALAIEHAAFNVDPAQLQDVVDKIQKAIEQNNNKRKVDNQTNTLREEDKK
                     RSETNAYADGKVTTPISLDETVFVEPIAPEAVRLAYSAVRFAREAAHNFTVEGATANN
                     TVYIDLSDWESFPVGTKVTFELDTEFAGESYVYENRRRVVGENNEYVKTHKTYNDFVR
                     QDGSIDTDNVPIKVVVRKSNKNIVIGYLPSIDFIEELDEYGRRKNLLENGRTQEEIEA
                     EIDKLRTLRKVVAENKKVKSSIALKTAGYVQVEYTVNNQGKRVPKADTVAEAMPNLLS
                     KAVPTYYEYNGTRLSPFTVFIDGKFIMTETDGKQEFPIDSVILHSNLKEKLKNGAIVL
                     MVPSAIPNKFIPLVLHSTKLGTEFANILADIIRIGLVASRNNGQGIQDLNEIDGKLYK
                     ELLKAGFNIAKRKTLKKFLKQFVYVADDDAVLDLNTHGLQFNPKTSELSIYSDGKVTT
                     LRLNDHKKLNSNIKTIASALANMYHSVNLKISGDIVLPVRNSNGLINITTVPVNKFLS
                     TKLLTLGREYQNSKDIPVYAIQPKILFSDPELPKRKSNSSTKSQTTVSSKDS"
BASE COUNT         1654 a          556 c          822 g         1087 t
ORIGIN      
        1 gtttagcaca atttggagca gctataaatc agctagcaat aggtattatt attggacaaa
       61 cagtacaggc tataggaact attttagcaa tacctgaaat agtttcagaa gattttaaaa
      121 tagataatcc tatttataaa attggtaaag gaataacaga gtgggcagaa aaagctactc
      181 caatatatca aactggagaa aggtggagtg attttgggca ttgggctcaa ggattagtta
      241 gtataggtag tgctgctagt tttctaattc ctggatatgg tatattcaaa gcagtaaatt
      301 atttaggtaa actattgaga ttaagtgagt ttactactct tgcattagca actggaattg
      361 gagcattagc tagcagacat atggaaggag ctatggaagc agatcaaaat tatcgagttt
      421 ctgaagaaaa attaaccaat atttggagaa ctggaagttt tgatgatgat agtgataatt
      481 ctattttagc aaatttatat aaacaaaaat ctattgatat tagtaatata ttaaataggg
      541 gagatttaaa aaaaggagat aaaattcgtg aaatagaaaa aattagtaat ttatatgata
      601 atgaagcaag taaactaata aatgatgcag ccaacatagg agctagaact gactatcttt
      661 taaactatgc caacttaata tttgatatag tacagtttgg agcactttcc aaattctggc
      721 aaaaaacttc cagaagaatg gctggagctg tggaaagaga gcttataaaa gctgaatcca
      781 aaactctagg tatgaaagct atatcagaag aagcactccc taaaactagg cttgatagga
      841 taataagtgg aatgggtaga ggggttaaag catactttcc agaaacaact gaaggactgg
      901 aagaagtatg gaatgagata tcagcacacg agggagaaag ggctgccatg atagcttctg
      961 gagtaaaaaa ggatgataat tctacctttg gtgagcgggt attagattat ttgggaatgg
     1021 atcaaacttt tgatgccttt gtgtttggag cacttggtgg tgttgctttt caatggttgg
     1081 gtggtaaaat aggatttggt gcagatagag acttactcaa taagaagatt gcagaaacag
     1141 cagcaagaga tagattacta aaggaaactg ctcagaaaat aaaagaaata caaactgatg
     1201 aaaaactaac cgcagaccag aaggcagaag aaattaaaca tcttgcatat aaaactggat
     1261 atcaaatagg attacaggct tccatatata ataatatgga tagggtagat gctcagatga
     1321 agtcccctgg acttatatct gatttagaga agttagaaat gggagataag aagagcatca
     1381 ctgaaactat ctcaaccatg caccaagcag ccctagaagc agaaaaaaat tttaataggt
     1441 acttttcaat agtaagaaat aaaaatatat cacataatgc aaaaatattt tttgcacagg
     1501 acgcagcaga tttacatagt acaatagtag ggattggaaa agaaatcaat gactataaaa
     1561 ctaaattagg agaagcaatt aagcagtatg aagaaataag tggtaaatca ttaaatgata
     1621 tagaaaagag tatactggaa ttaactgggt taaatgttgc tattgatgaa cttaataagt
     1681 attccactaa tacagttaat agtaaaaatt caagagaatt ctctaaaaac attattaaaa
     1741 aactggaaca aaggaaactg gaactagaaa agcagatagg aaatacaagg gttcagggaa
     1801 aagatattgg gggagtacat ataatgactg tactaaagta ccccaatcat ttagcaaata
     1861 tttatacctt agaagcagca agggaactac atagtgaaaa acttcaaaag ttaatgactg
     1921 aagaaggtca aaaagagtat gaaaaacaaa taaaaaagga aatagaagaa gaaaggaaaa
     1981 agaaaacaga agaaagacaa gctagggagt acaataagaa caaagataaa acaaagtctg
     2041 aaaatcaaca aaatacttct caacaatcca ctgaaacacc ttctactagt aaatcaacta
     2101 ataatgaaac aggtgaaaca ggtgaagcag aagaaacagg aaaagcagaa gaaccagaag
     2161 aaactagtaa gaaagatttg ccaaatattg ttccaggaag acctggagta ataccagagg
     2221 aagaggaaaa gaaaaaggat gatgacagta agagtggtgg aaaagaaaca ggccatgagg
     2281 tagaaccagg tgaagctgga gtatctactg aaccatatga cctacctgat ggacgtattg
     2341 agggtactcc gtatgaagta aaagatagaa ccctgtatta taatggacaa cctgtaggaa
     2401 atccaaataa aaaggaacta ggagcattag ctatagaaca tgctgctttt aatgtagatc
     2461 cagctcaact acaggatgta gtggataaga tacagaaagc tatagagcaa aataataaca
     2521 aaagaaaggt agataatcag actaataccc tcagagaaga agataaaaag agaagtgaaa
     2581 ctaatgccta tgctgatggg aaagtaacta ctccaatttc tcttgatgaa acagtattcg
     2641 tagaacctat agctccagaa gctgtaaggt tagcatatag tgctgtaaga tttgctagag
     2701 aagctgccca taactttact gttgaagggg ctactgctaa taatactgtt tatatagatt
     2761 tatctgattg ggaatcattc cctgttggaa ctaaagttac ttttgagctt gatacagaat
     2821 ttgcaggaga atcctatgta tatgaaaatc gtaggagagt tgtaggagaa aataatgaat
     2881 acgttaaaac ccataaaacc tataatgact ttgttagaca ggatggaagt attgatactg
     2941 acaatgttcc tatcaaagta gtagttagaa agtctaataa aaatattgtt attgggtatc
     3001 tcccttctat tgatttcata gaggaactag atgagtatgg aagaagaaaa aacctattag
     3061 aaaatgggag aactcaggaa gaaattgaag cagaaataga taaattaaga actctacgta
     3121 aagttgttgc agaaaataaa aaagttaaaa gtagtattgc acttaaaact gctgggtatg
     3181 ttcaagtaga atatacagta aataaccagg ggaaaagagt ccctaaagct gatactgttg
     3241 ctgaggctat gcccaaccta ttaagtaaag cagttcctac ctactatgaa tacaatggaa
     3301 ccaggctatc tccatttact gtatttatag atggtaaatt tataatgact gaaactgatg
     3361 gtaaacaaga atttcctatt gatagtgtta ttttacattc taaccttaaa gaaaaactga
     3421 agaatggagc tatagtactt atggttccaa gtgctatacc taataaattc atcccacttg
     3481 ttctacattc aactaaattg ggaactgaat ttgctaatat cctagctgat ataattagaa
     3541 taggattagt tgcttcacgg aataatggac aggggataca ggatttaaat gaaatagatg
     3601 gaaaattata taaagaacta ttaaaggctg gatttaatat tgcaaagaga aagactctta
     3661 aaaaattctt aaagcagttt gtgtatgttg ctgatgatga tgcggtatta gatttaaaca
     3721 cacatgggtt acagtttaat cccaagacta gtgaactcag tatttatagt gatggtaaag
     3781 taactaccct tagattaaat gaccataaaa aacttaatag taatattaaa accatagcca
     3841 gtgcattagc taacatgtat cattctgtta atcttaagat ttctggggac atagtattgc
     3901 cagtaagaaa ttctaatggc ttaataaata ttaccactgt acccgtaaat aaatttctca
     3961 gcactaaact tcttacatta ggaagggagt accaaaattc taaggacatt ccagtatatg
     4021 caattcaacc aaagatacta tttagtgacc ctgaactacc aaaaagaaaa tccaattcat
     4081 caacaaaatc tcaaactact gtaagttcaa aagattcca
//
LOCUS       DAOSAB010000028         4092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_14649_length_4092_cov_3.104533, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000028 DAOSAB010000000
VERSION     DAOSAB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14649_length_4092_cov_3.104533"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..2499
                     /locus_tag="PK432_00970"
     CDS             82..2499
                     /locus_tag="PK432_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020278978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOK59531.1"
                     /translation="MSEESVDISAYFSQRAKDIIQMAAEKVIDSGSRNIDTEHLLWAA
                     INYDDVVVRVLKELKIDNELLQDQLNEVIGSDEGITHGTPGLTPRAKEVLQLAFYESQ
                     ELGHHYIGGEHILLGLIEEREGIAAQIFEKYGISLTQARQAVIKVVGEGDESGEKVEE
                     SSVTPTLDKYSRDLTKLAKEGKIDPVIGRSDEITRVIQILCRRKKNNPVLIGEPGVGK
                     TAIAEGLAYRIATGNVPEVLLHKTVKELDVAALIAGASHRGEYEERIQKVLKELEKSG
                     RDTILFIDELHTIVGSGAPERQMDLSNMLKPALARGDIQIIGATTLSEYKKYIEKDAA
                     LERRFQIVLVGEPTVDQTIEILRGLRDRYEAHHRVKISDEAITAAAVLSEKYIQDRFL
                     PDKAIDLIDEAASKVKLEVSSEPEELRELKRKIESMEKEREALTRSGNYEKAAEIKVE
                     IEKLKTKLEPLVEKWNKVKGTGTPVVKTEDVAEVLSRATGIPVTELCEEERDRLLKLE
                     ETLHQRVVGQDEAVKAVSEAVRRSRVGLKDPRKPIASFLFMGPTGVGKTLLAKALAKQ
                     IFGDEDAIIRIDMSEYMEKFSVSRLIGSPPGYVGYEEGGQLTEAVRRKPYCVILLDEI
                     EKAHSDVFDILLQVLDDGRLTDAQGRTVSFKNTIIIATSNIGANKIQEFVKKGEKDWN
                     KLKEELMADLKSYFKPEFLNRLDDIIVFKSLDKKQIKEIARILLEDVKGLVKAQGMEI
                     EFDDSVIELVASIGYDPEFGARPMERVIQNVVENPLADALLEGKFNKGDTIFATVKDG
                     KIVFEKK"
     gene            2874..3338
                     /locus_tag="PK432_00975"
     CDS             2874..3338
                     /locus_tag="PK432_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOK59532.1"
                     /translation="METKRRKNTNKELATTSYSPVRNIIDDFFTLPSVFDSLVNRSIM
                     PSYNTLMADVWEEGDNYFVKMAMPGISKKDITIELDGDTVRIRGEKKEEEKEEGKKKY
                     YFRSLESQFEQVFNLPTVIDADKAEASVKDGVLLIKLPKAKEYMPKKVEIKE"
     gene            3445..3909
                     /locus_tag="PK432_00980"
     CDS             3445..3909
                     /locus_tag="PK432_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOK59533.1"
                     /translation="MSKNIKRILGTVSVFVLFFSLVSAGRIYAQTQELTNEEEFNLDY
                     EYDQPVLYDYQDEKAINTNRECQCRYNNLNGYGITVIFFLFFFIFAIVIASLVFNIVM
                     IVDCINRDEKDFKDRTIWLVVLIVGGFTGFGLIVSLVYFFAVKKKLDVIEKK"
BASE COUNT         1450 a          483 c          895 g         1264 t
ORIGIN      
        1 tgtataacag tcagttccaa ttgactgatg atgattaagt ggtatatatt cttgtagtct
       61 ttaatttatt tattaaatga tatgtcagaa gagtcagtag atatatcggc atatttctca
      121 caaagagcta aagatattat acaaatggct gctgagaagg ttattgatag tggaagtaga
      181 aatattgata cagagcattt actgtgggca gccataaatt atgatgatgt tgttgttaga
      241 gtacttaaag agctaaaaat tgataatgaa ttgctgcaag atcaactgaa tgaagtaatt
      301 ggaagtgatg aaggtattac acatggtact ccagggctta ccccaagggc aaaggaggta
      361 ttacagcttg cattttatga atctcaagag ttaggtcatc actatattgg aggggagcat
      421 attcttcttg gtcttataga agaaagggag ggtattgctg cacagatatt tgaaaagtat
      481 gggatatctc ttacacaggc aagacaggca gtaataaaag ttgtgggaga aggagatgaa
      541 agtggtgaaa aggttgagga atcttctgtt actcctacac ttgataaata ttcaagggat
      601 ttaactaagc tagccaagga agggaaaata gatcctgtaa ttggaagaag tgatgagata
      661 actagagtaa tacaaatact ttgtagaaga aaaaagaata atcctgtttt aataggagag
      721 ccaggagtag gtaaaactgc aattgcagaa ggacttgcat atcgtattgc aacgggaaat
      781 gtccctgagg tacttcttca taaaacagta aaggagcttg atgttgcagc gctcatagca
      841 ggagcaagtc acagaggaga gtatgaagag agaatacaaa aagtacttaa ggagctagag
      901 aaatcaggaa gagatacaat tctttttata gatgaacttc atacaattgt aggaagtggt
      961 gctcctgaaa gacaaatgga tttatctaat atgcttaaac ccgcacttgc aagaggagat
     1021 atacaaatca ttggagcaac tacactaagt gagtataaaa agtatattga gaaagatgca
     1081 gcacttgaaa gaagattcca aatagtactt gtaggagaac ctactgtaga tcaaacaatt
     1141 gaaatactta gaggtttaag agatagatat gaggctcacc atagagtaaa gatttctgat
     1201 gaggcaataa ctgctgctgc tgtactatca gaaaagtata tacaagacag attcttacca
     1261 gataaagcaa ttgatttaat tgatgaagct gcaagtaagg ttaagctgga agtatcttct
     1321 gagcctgaag agttaagaga gttaaaaaga aagattgaga gtatggagaa agagagagaa
     1381 gctctcacaa ggagtggtaa ttatgagaaa gcagctgaga taaaagtgga gattgagaaa
     1441 cttaaaacta agcttgaacc acttgttgag aaatggaata aggtaaaagg aactggtaca
     1501 ccagttgtta agacagaaga tgtagcagag gttctttcaa gagcaacagg tattcctgta
     1561 acagagctgt gtgaggaaga aagggataga cttcttaagc ttgaagaaac actacatcaa
     1621 agagttgtag gtcaagatga agctgtaaaa gcagtttcag aggcagtaag aaggtctaga
     1681 gtagggctta aagatccacg aaagcctatt gcttcctttc tttttatggg acctactggt
     1741 gtaggaaaaa cattgcttgc aaaagcattg gctaagcaga tatttggtga tgaagatgca
     1801 attattcgta tagatatgag tgagtatatg gagaagttct ctgtatccag acttattggt
     1861 tctcctccag gatatgttgg ttatgaagag ggaggacagt taacggaggc tgttagaaga
     1921 aaaccatact gtgtaatact cttagatgag attgaaaaag ctcacagtga tgtttttgat
     1981 attcttcttc aagttcttga tgatggcaga cttactgatg cgcagggaag aacagttagt
     2041 tttaaaaata ccattattat tgcaacaagt aatattggtg caaacaaaat acaagagttt
     2101 gtaaaaaagg gagaaaagga ttggaacaaa ttaaaagaag agttaatggc agatcttaaa
     2161 tcatatttta agcctgaatt tcttaatagg cttgatgaca ttattgtttt taagagctta
     2221 gataaaaaac agattaaaga aattgcaaga atacttttag aagatgttaa aggtttagta
     2281 aaagctcaag gtatggagat tgaatttgat gatagtgtaa ttgagcttgt tgctagtatt
     2341 ggttatgatc ctgaatttgg tgcaagacct atggaaagag taattcaaaa tgtagtagag
     2401 aatcctttag cagatgcatt gctagaaggg aaatttaata aaggagatac tatttttgca
     2461 acagtaaagg acggtaagat agtttttgag aaaaaataag ttatatacct cttgacaatt
     2521 agcactcaat atgctacatt gctaatagga caatgactaa ggagtctgtt tgagatttct
     2581 tggttatttc caagtaattt ctacagattt tcctttgtct tgttcacctt tatatactat
     2641 ttaacttgta aaagttggta gtatatgaag aggacaatct tcttttagtg gcttcatcta
     2701 atgccacaag caattttaac aatttaaaat ttgcgtttac ctgatgcagg tcaggtaata
     2761 ctgaatatca attcagtatt gtggttgtaa gtcactaaga aagccatgtc tcctcggctt
     2821 ttaaaagatt gaggagagct actcttttat ctaaattgtt acttagtgtg cttatggaga
     2881 ctaaaagaag aaaaaataca aacaaagaat tagctacaac atcttactct cctgtaagaa
     2941 atataattga tgatttcttt acacttcctt ctgtatttga ttcattagta aatagatcaa
     3001 ttatgccaag ctacaataca ttaatggcag atgtatggga ggaaggagat aattactttg
     3061 taaaaatggc tatgcctggt atatctaaga aggatattac aattgagcta gatggagata
     3121 cagtaagaat tagaggagag aagaaagaag aagaaaaaga agaagggaag aaaaagtatt
     3181 actttagaag tcttgagagt cagtttgagc aggtatttaa tctgccaact gtaattgatg
     3241 ctgataaagc agaggcttct gtaaaggatg gtgttttact tattaaactt cctaaggcaa
     3301 aagagtatat gcctaagaag gtagagataa aggagtaagt tgtacaggga gagggcttag
     3361 ctctctccct tctatatact cttaagattt tacaatgctc ttttttttgt atactatata
     3421 gtattaatat ctaaatgcat aaaaatgagt aaaaatataa agagaattct tggtactgtt
     3481 tctgtctttg ttttattctt ttctttagtt tctgcaggta gaatatatgc tcaaacacaa
     3541 gagttaacaa acgaagaaga gtttaacctt gattatgagt atgatcagcc tgttttatat
     3601 gattatcaag atgagaaagc aataaatact aatagggaat gtcaatgtag gtataacaat
     3661 ttaaatggtt atggtattac tgttattttc tttttgtttt tctttatttt tgctattgtt
     3721 atagcttctc ttgtatttaa tattgttatg atagttgatt gtataaatag ggatgaaaaa
     3781 gattttaagg ataggactat atggcttgtt gtattaatag taggtggttt tacaggtttt
     3841 gggttaatag tttctttagt atatttcttt gctgttaaga agaaattaga tgttatagag
     3901 aagaagtagt tttaattgac agtgggcata tatttttgtt aatctaaata gtatgaagaa
     3961 gttaagtagt ttagagattg tattgctatt aataatagca gtactttcta ttggtatatt
     4021 tgtagtagga gatactttag cagaacaaag tggtgatact cctgattctg gggagaattc
     4081 aatattaaaa ga
//
LOCUS       DAOSAB010000029         4067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_14753_length_4067_cov_2.739531, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000029 DAOSAB010000000
VERSION     DAOSAB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14753_length_4067_cov_2.739531"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK432_00985"
     CDS             complement(<1..210)
                     /locus_tag="PK432_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59534.1"
                     /translation="MSYNYTLKYRTFDQLLDAVRIDFTQYDLTNYIEPQQLIKVAKRL
                     SYDLGLRILMTKEAVLGVKKGSVRLT"
     gene            complement(215..886)
                     /locus_tag="PK432_00990"
     CDS             complement(215..886)
                     /locus_tag="PK432_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59535.1"
                     /translation="MTTIGETVSRVRNTVKGVKEDAFLTDRFLYSLIIKYANLAIRKL
                     DNENKLMRIQSLFEVLPVVDLIEVSKIEAQCAGIKTNCTIMRTKEHIPEPLEGAEGPL
                     IRSVTSLDGSIILTNTSPSTYSLMTGQPTFKYNKTSYYWYLEQHLYFPNIEWDAVKID
                     GIFTESTAKFHCDGDVCKPRQDDRLTIPDFLYAEVESQVLKDLGFMIQSPTETQDDKM
                     NPLRS"
     gene            complement(892..1524)
                     /locus_tag="PK432_00995"
     CDS             complement(892..1524)
                     /locus_tag="PK432_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59536.1"
                     /translation="MSTSKNIKKPDLTAPRCRADVHKIICRIKNSQAKEYSHKFLEEF
                     KKEYPQYKDLTNDQAMDILEVFHGKLWNHSLNNRDGVELPKGLGYIFLGTCASAKKPS
                     IDFKVLIEEGIKKKHLNFESDNKLAKIFYTNFSTKYKFRNREMWSFTATREFKRSVPE
                     VYRANWKIYVEVESGRSISKYINRIMKNNYFRNYTEKYEVPSSYNEFDLT"
     gene            complement(1636..2364)
                     /locus_tag="PK432_01000"
     CDS             complement(1636..2364)
                     /locus_tag="PK432_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59537.1"
                     /translation="MLTPGTITVNFTAEYAGPHRICWRQCGVGQYVCTNIINCVGGGN
                     VCSAVISVMVNPESCDPVCFEGYIQATCNPEGSSTGQVPWTTTFTPNPACKLYQLRYD
                     PSEPCLNPPQLGPVIPTVDMQLNCNGTARPDVVLYCESAILLCSASIIPNLPAGYEMT
                     LFDGCCYDCTQYTVTITPACPECLLDGSSMYYIDCNTRELKRIDFTGNVPVVVPPFCA
                     VTGSVSLQLSVEATGVVNTIGACP"
     gene            complement(2381..3574)
                     /locus_tag="PK432_01005"
     CDS             complement(2381..3574)
                     /locus_tag="PK432_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOK59538.1"
                     /translation="MSNCSKCGSCEDCGCVPHGMTTPNYCPSDLPPCPQPSPCNETFD
                     SKCVIYTGDNLPCIGAETGNSVEEVILSLNTKLTPFFCIQCVSLVVPANASTGIPNNQ
                     TLTWNIVPGATSYDVYFGTSSTNPPLVSAGQIMTSYTSPYPLLPNTTYYWKVVPKNSG
                     GAASNCPIFNFSTVALACVNPLSYMLDYVKRLQTGAFNVTTFIQDLNDYLTEGELITN
                     CNFCCPDCTQTKRYVLASAPVYAAYYNQFYDSPNCLPVCCTEVDASLTAMALTLNPGS
                     PSLATAFAALPPVTNCCGTNFSECSQKLKLELGTAVNTVYQGAGIVEESTINDTTELC
                     ILTDFLDTLTGITDADKANIVVALLTRGFVVQCRPEGTIIASVQAYTTYITAAQSGCL
                     CYKPC"
     gene            complement(3587..>4067)
                     /locus_tag="PK432_01010"
     CDS             complement(3587..>4067)
                     /locus_tag="PK432_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59539.1"
                     /translation="TISDSHGTTVTYSIDIFSTINSPTGYVIDLNGTRLILTDNLLIQ
                     ANPSFTSRNNNSICASYISYTVVNVLVCPTVSYTPAADFIGFSFISDASSKVYTLEIY
                     ALGSVTPLSTQAFISNGVNTYTGRFNALTPATTYQLRVKINTTDSEKTCEFTNVTTL"
BASE COUNT         1351 a          728 c          671 g         1317 t
ORIGIN      
        1 agttaatcta acacttcctt tttttactcc aagaacagct tctttagtca tcaatattct
       61 gagaccaaga tcataactta atcttttagc aaccttaatc aattgttgag gttcaatata
      121 attggttaga tcatattggg taaaatcaat tctcacagca tctaatagct gatcgaatgt
      181 tctgtatttt aatgtatagt tatagctcat ttttttatga tcttaaagga ttcatcttgt
      241 catcctgagt ttcagtaggt gattgaatca tgaaaccaag atcttttaat acttgtgatt
      301 ctacttccgc atataagaaa tccgggatag ttaatctatc atcctgacgc ggtttacaaa
      361 catcaccatc acagtgaaac ttagcagtag attctgtaaa tataccatca atctttactg
      421 cgtcccattc aatattagga aaatataaat gttgttctaa ataccaatag tatgaagttt
      481 tattatattt aaaagtaggt tgaccagtca taagtgaata agtactagga ctggtgttag
      541 ttaaaattat agatccatct agagatgtta ctgatctgat aagcgggcct tcagctcctt
      601 ctaatggttc tggtatatgc tctttagttc tcataattgt acagttagtt ttaatacctg
      661 cacactgagc ttctatctta cttacctcaa taagatctac tacaggaagt acttcaaata
      721 aactttgaat acgcataagc ttgttttcat tatcaagctt tctgatagct aggttagcat
      781 acttaatgat aagactgtat aaaaatctat cagtaaggaa tgcatcttcc ttaactcctt
      841 ttactgtgtt cctcacacgg gatactgttt caccaatagt tgtcattgtg tttaagttaa
      901 atcaaattca ttataagagc ttggtacttc atacttttct gtataatttc tgaaatagtt
      961 attcttcatt attctattaa tatatttaga tatactccgg ccactttcta cttcaacata
     1021 aattttccaa ttagctctat acacttcagg tacagatctc ttaaattctc ttgttgcagt
     1081 aaaactccac atctctctat tacgaaactt atacttagta ctaaagtttg tgtaaaagat
     1141 cttagctaac ttattatctg attcaaaatt aagatgcttc tttttaatac cttcttcaat
     1201 tagaacttta aagtctatag aaggcttctt cgcagacgcg catgtaccta agaaaatata
     1261 gcccaatccc ttaggaagtt ctacaccatc tctattattt aagctatggt tccaaagctt
     1321 accatgaaat acttctagaa tatccatagc ttgatcatta gtaagatctt tatactgagg
     1381 atattctttc ttaaactctt ctagaaattt atgactatat tctttggcct gactattttt
     1441 aattctgcaa attattttgt gtacatcagc acgacatcta ggagcagtta gatctggctt
     1501 tttaatattt ttacttgtac tcatatacat ataaatataa tcaatatttg caaatatagc
     1561 aaaaacccca gtataattta accggggctc tcacttatta gtcatgttga aaccaacaaa
     1621 tcatgactta attttttatg ggcaagcacc tatagtattt acaacacccg tagcttctac
     1681 agataactgt aatgaaacag atcctgtaac ggcacagaat ggtggaacta caacagggac
     1741 attaccagta aagtctatac gttttaactc tctagtatta caatcaatgt agtacataga
     1801 tgaaccgtct aaaagacatt ctggacaagc cggtgtaatg gttactgtgt attgtgtaca
     1861 atcataacaa caaccatcaa aaagtgtcat ttcataacca gcaggaagat tagggataat
     1921 gctagcagaa caaagaagta tagcactttc acaataaagt accacatcag gacgagctgt
     1981 accattacaa tttaactgca tatcaacagt tggaataaca ggtcctaact gtggagggtt
     2041 caaacaaggt tctgaaggat catatcttaa ttgatataac ttacaagctg gattaggtgt
     2101 aaaagttgtt gtccaaggca cttgacctgt agaagatcct tcaggattac aagttgcttg
     2161 aatataccct tcaaaacaaa ctggatcaca actttctgga ttaaccataa ctgaaattac
     2221 ggcactgcat acatttccac ctcctacaca atttataatg tttgtgcaca catattgacc
     2281 tactccacac tgtctccaac agattcggtg aggtccagca tattcagcgg tgaaatttac
     2341 agtaattgtt cctggtgtta gcataattgt ttatttaaga ttaacatggt ttataacata
     2401 agcatccgct ttgagctgca gtgatataag ttgtataagc ttgtacactg gcaataatag
     2461 ttccctctgg acggcattgt acaacaaatc ctcttgttaa aagagcaact acaatgtttg
     2521 ctttatctgc atcggtgata ccggttaaag tatctaaaaa gtcagtaagt atacaaagtt
     2581 cagtagtgtc atttattgta gattcttcta caatacctgc accctgatac actgtattta
     2641 cagctgttcc taattcaagt ttaagcttct gactacactc actgaagttt gttccacaac
     2701 agttagttac aggaggtaac gctgcaaaag ctgtagctaa tgaaggactt cctgggttta
     2761 atgtcaaggc catagctgtt aatgaagcat ctacttcagt acaacataca ggaagacagt
     2821 taggagaatc ataaaactga ttgtaataag cagcatatac cggggcagaa gccaagacat
     2881 atctctttgt ttgagtacaa tcaggacagc agaagttaca gttagtgatt aattcaccct
     2941 cagtcaagta atcattaaga tcttgaatga aagttgtaac attaaatgca ccagtctgta
     3001 atctttttac ataatctaac atgtagctta gaggatttac acaagctaaa gctactgttg
     3061 aaaaattaaa tattggacaa ttagatgctg caccacctga attcttaggc actactttcc
     3121 aataataagt tgtattagga agtaatggat aaggactggt ataagaagtc attatctgac
     3181 ccgctgatac caaaggagga ttagtagaac tagttccgaa gtaaacatca taagatgtgg
     3241 cacccggtac aatgttccaa gttaatgttt gattattagg gattcctgtt gaggcatttg
     3301 ccggcacaac aagactaaca cactgaatac agaagaaagg tgtaagctta gtatttaaag
     3361 agagaattac ttcttctact gaattaccag tttctgctcc tatgcacggt aagttatctc
     3421 cagtatagat aacacatttg ctatcaaatg tctcattaca tggtgaaggc tgtggacaag
     3481 gaggaagatc tgaaggacaa tagttaggag tggtcatacc atgaggtaca cacccacaat
     3541 cctcacaact tccgcatttt gaacaattac tcattttgtt tctttattaa agggttgtaa
     3601 cattagtaaa ttcacatgtt ttttcagaat cggttgtatt aatcttaaca cgaagctgat
     3661 aagttgtagc aggagttaat gcattaaagc ggcctgtata agtattaaca ccattagata
     3721 taaatgcctg agtacttaaa ggagttactg agcctaaagc atatatctca agagtataaa
     3781 cttttgaaga agcatctgaa ataaagctaa atcctataaa atcagcagct ggcgtataag
     3841 atacagtagg acaaactaac acatttacca cggtataaga tatataagat gcacatattg
     3901 aattattatt tctactagta aagctaggat tagcttgaat aagtagattg tcagtaagaa
     3961 tcaatcgtgt accattaaga tctataacat atcctgtagg agaattaatt gtactaaata
     4021 tatcaataga atatgtaaca gtggttccat ggctatcaga gatagtc
//
LOCUS       DAOSAB010000030         4062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_14782_length_4062_cov_3.149738, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000030 DAOSAB010000000
VERSION     DAOSAB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14782_length_4062_cov_3.149738"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK432_01015"
     CDS             complement(<1..211)
                     /locus_tag="PK432_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOK59540.1"
                     /translation="MPTINQLVRKPRKSKSTKSKHIALATNYNPLKNRYKRANNPFKR
                     AVCLQVKTMTPKKPNSALRKVARVRL"
     gene            complement(237..>4062)
                     /gene="rpoC"
                     /locus_tag="PK432_01020"
     CDS             complement(237..>4062)
                     /gene="rpoC"
                     /locus_tag="PK432_01020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOK59541.1"
                     /translation="SPEQILDWSYGEVTKAETINYRTFRAEPDGLFCEKIFGPTKNYE
                     CYCGKYKKIRYKGIVCDKCGVEVTTKDVRRERMGHINLAVPVAHVWFAYGIPNKMSIV
                     LDMSHKKLLSVIYYTRYMVVEIESEKRKDMLSKLEAIKKDELKNLKDELEEELKGTEV
                     AYDEDIKAEKKKKEKNNFKIDQLEHKKRQGIAKVRREYAEREEEIENFYSKLQQIIEK
                     VEVGSVLSEDEYIDLEDRGLLFFNAMMGAEAIKKLLENLNIDEELEKLRRQVKKEKGD
                     KKLVTIRRIQYLEGFQQNKLDPSWMVIDVLPVLPPDLRPIIPLPGGKFATSDLNDLYR
                     RVINRNNRLKRLIEIGAPDVILRNEKRMLQESVDAVIDNSHRPSKPMMNSKRLPYQSL
                     TDDLRGKKGIFRKNLLGKRVDYSGRAVIVGDPSLKLDQCGIPKSVLLEMFKPFVIHEL
                     LDKEIAPNVRVARDLVEEEDSMIWDILEKVIEGKPVLLNRAPTLHKYSIQAFYPKLVE
                     GEAIRLHPLVCKAFNADFDGDQMSVHLLLTDEALKEGKEKIMASKNIVNISNGEVLAT
                     PSKDMLLGYYLLTDKNEVEKPKIFASSDLAIRAYEEDIIGINDEILVKINDEVISTSV
                     GRCIFNSIMPQGYKFINERVSGGTVSAIIDDIKDKYSQDVVVELLDNLKDMGFKYATD
                     LGFSFGMKDCNLDFDIKSRIKEIEEKDLQLQENYLQGLITEKEKVKISTEMWNDFATK
                     IADEAWEQLDKNNPIYEMVESKANGGKIQARQVLTIKGIVKDSRGNSIPLPIKGNYRD
                     GLNAFEYFVAANGGRKGIADRSLRTSSSGYLTRKLVDVAHEVIIRMEDCGYEGEGLVM
                     RKNDDRRMSFEKRIFGRVVAQDVVDSKGEVIVKKNESITREIVDKIMSEGIEEIYVRT
                     PILCKAPLGICKKCYGLNIENGQEIEMGKAVGVIAAQSIGEPSTQMTMQTFHKGGVQT
                     TDIVQGLPRVEELFEARTPKAEADIATVNGKVHIDRAEDDSAVITIIGEKNVVKDIVI
                     SDAKSVLVSDGQQVKAGQVLYIDSDGAEKQSPFSGVVKIEGGILTLSGSMKAEETVSV
                     LPNVEILVAEGDEIKAGTQLTAGSVDPKKLADVAGLLVAQKYILDQVQKVFNEQAVPI
                     DDIHLEVVIRQMARLGRVLESGDTNYLVGSMVNRFITDVKNELLLKENKRKVLVSPKL
                     LGIKTSALYTESFLSAMSFQEQVRVLTNTAILGKTDYLRGMKENVIIGRRIPSGQEAE
                     IKDIYSLEEVKI"
BASE COUNT         1178 a          890 c          482 g         1512 t
ORIGIN      
        1 aaagtcttac tcttgcgact tttcgcaatg cagagtttgg ctttttaggt gtcattgttt
       61 taacctgaag acatactgct cttttaaaag ggttattagc ccttttatac ctgtttttta
      121 aagggttata atttgttgcc aatgcaatat gcttgctctt ggtactcttt gattttctag
      181 gttttcttac taactgatta atagtcggca tgagttatat tatacaataa aatcaattaa
      241 attttaactt cctctaaact ataaatatct tttatctcag cttcctgccc tgatggtatt
      301 cttctcccaa ttattacatt ctctttcatt cctcttaagt aatctgtctt accaagtatt
      361 gcagtatttg ttaaaactct aacctgctct tggaatgaca tagcagaaag gaagctctca
      421 gtatacaatg cagatgtttt aatgccaagc agttttggag acactagaac ttttctctta
      481 ttctctttca aaagtaattc attttttaca tcggtaataa atctattaac catcgagcca
      541 accaaataat tagtatctcc tgattctaat actctcccta gtcttgccat ctgtctaata
      601 acgacctcca aatgtatatc atcaatagga actgcctgct cattaaatac cttctgaact
      661 tgatccaata tgtatttttg agcaacaagt aatcctgcaa catcagctag tttcttagga
      721 tccacagaac ctgctgttaa ttgtgtccct gccttaattt catctccttc tgcaacaagt
      781 atttctacat taggaagtac agaaacagtt tcctctgcct tcatactacc agaaagtgta
      841 agaattcctc cctctatctt aacaactccg ctaaatggag attgcttctc agctccatca
      901 gaatcaatgt aaagaacctg acctgctttt acctgttgac catctgaaac aagaacactc
      961 tttgcatcag aaattacaat atccttaaca acatttttct caccaataat tgtaataact
     1021 gctgaatcat cttctgctct atcaatatga acttttccat ttactgttgc aatatcagct
     1081 tctgcctttg gagttctagc ttcgaaaagc tcttctactc ttggaagccc ttgtacaata
     1141 tcagttgttt gaacacctcc cttatgaaat gtctgcatag tcatttgagt acttggctct
     1201 ccaattgatt gtgcggctat aacaccaaca gctttaccca tttctatctc ttgcccattc
     1261 tcaatattta atccataaca tttcttacaa ataccaagag gagctttaca aagtatagga
     1321 gttctaacat atatctcctc tataccctca ctcataattt tatcaacaat ctctcttgta
     1381 atactctcat ttttctttac aattacttct cccttagaat caacaacatc ctgagcaaca
     1441 actcttccaa atatcctttt ctcaaaactc attcttctat catcattttt tctcattaca
     1501 agaccctcac cttcatatcc acaatcttcc attcttataa ttacctcatg tgctacatca
     1561 acaagctttc tagttaaata cccagaagat gaagttctta atgacctatc agcgatacct
     1621 tttcttcctc cattagctgc aacaaaatat tcaaaagcat taagcccgtc tctataattt
     1681 cctttaattg gaagtggaat agagtttcct ctagaatctt taacaattcc tttaatagta
     1741 agaacctgtc ttgcttgaat ctttcctcca tttgcctttg attctaccat ctcataaatt
     1801 ggattatttt tatcaagctg ttcccatgct tcatcagcaa ttttagttgc gaaatcattc
     1861 cacatttcag tagatatctt tactttttcc ttctctgtaa ttaatccctg taggtaattc
     1921 tcttgaagtt ggagatcttt ttcttctatt tccttaattc ttgatttaat atcaaaatca
     1981 agattacaat ctttcatacc aaaggaaaaa cctaaatctg ttgcgtattt aaaacccata
     2041 tctttaagat tatccaaaag ctcaacaaca acatcttgtg aatacttatc tttgatatca
     2101 tcaattattg ctgatactgt tcctccacta actctttcat taataaactt atacccttgt
     2161 ggcattatac tgttaaatat acatctccca acagaagtac ttataacctc atcatttatc
     2221 tttacaagga tttcatcatt aatacctata atatcctcct catatgctct aattgctaag
     2281 tctgaagatg caaaaatctt tggtttttca acttcatttt tgtcagtcag aaggtagtaa
     2341 ccaagaagca tgtcttttga tggagttgct agaacttcac catttgagat attaacaata
     2401 ttcttagaag ccataatctt ctcttttcct tctttcaatg cctcatcagt taagagaaga
     2461 tgtactgaca tctgatcccc atcaaagtct gcattaaatg ctttacatac caatggatgc
     2521 aatcttattg cttctccttc aactaattta gggtagaatg cttgaatact atatttatga
     2581 agtgtaggag cacgattaag taatacaggt ttaccttcaa taaccttctc taagatatcc
     2641 caaatcatac tgtcttcctc ttcaactaaa tctcttgcaa ctcttacatt aggtgcaatt
     2701 tctttatcta aaagctcatg tataacaaaa ggcttgaaca tttcaagaag aacagattta
     2761 ggaataccac actgatctaa tttaagactt gggtcaccaa caattactgc acgtccagaa
     2821 taatcaactc tctttcctaa aagattcttt ctaaatatac ctttctttcc ccttaaatca
     2881 tctgtaagag attgatatgg gagtctttta ctattcatca taggctttga tggcctgtgt
     2941 gaattgtcaa ttacagcatc aacagattcc tgaagcattc tcttttcatt tctaagtatt
     3001 acatcaggag ctccaatctc tataagtctc tttaaacggt tatttctatt aataactctt
     3061 ctgtataaat cattaagatc tgatgttgca aactttcctc caggaagagg aataattggt
     3121 cttaaatctg gtggaagaac aggaagaaca tctattacca tccagcttgg atctaatttg
     3181 ttttgttgga aaccttcaag atattgtatc ctccttatag ttacaagctt tttatcgcct
     3241 ttctctttct tcacctgtct tcttaatttc tcaagttctt catcaatatt taaattctct
     3301 aaaagctttt taattgcttc tgcacccatc attgcattga agaaaaggag ccctctatct
     3361 tccaaatcta tatattcatc ctctgaaaga acagaaccta cttctacttt ttcaattatt
     3421 tgctgaagtt tagagtagaa gttctctatt tcttcttctc tttcagcata ttctcttctt
     3481 actttagcta ttccttgtct ttttttatgc tctaactggt ctattttaaa attgtttttc
     3541 tctttctttt tcttttctgc tttaatatct tcatcatatg caacttcagt cccttttaac
     3601 tcttcttcaa gttcatcttt aagattcttc aattcatcct ttttaatagc ctctaacttt
     3661 gaaagcatat cttttctctt ctcactttct atttcaacaa ccatatatct ggtgtaatag
     3721 attacagaaa gaagcttttt atgagacata tctaaaacta tggacatctt attaggtatt
     3781 ccataagcaa accatacatg agcgacaggt acagctaaat taatatgccc cattctttct
     3841 cttctcacat ctttagttgt aacctcaaca ccacatttat cacatactat tcccttatat
     3901 ctaatctttt tatacttacc acaataacat tcatagttct ttgtaggtcc aaatatcttt
     3961 tcacagaaaa gaccatctgg ttcagccctg aatgttctgt agtttatcgt ttctgctttt
     4021 gtaacttccc catatgacca gtcaagtatc tgctctggcg at
//
LOCUS       DAOSAB010000031         3995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_15099_length_3995_cov_3.727411, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000031 DAOSAB010000000
VERSION     DAOSAB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15099_length_3995_cov_3.727411"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PK432_01025"
     CDS             <1..772
                     /locus_tag="PK432_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOK59542.1"
                     /translation="IETIRTKKNEEFRNKYRQVDLLIIDDIQFVETYPKTQEELFHTF
                     NALYQANKQIVLASDRAPKDIKNITDRLRTRFEGGMVCDIQPPEYETRLAILQLIAME
                     KRVPISKDILVLIAKNIENSVRELEGALNKVITLVRLGMNPSYDDVARFLQIDIESKR
                     KRVTPEKVINVVSEVFDVPVNMIKGQKRTSDIAQARQVVMYLLRTQLELPLCTIANAV
                     GRKDHTTVIYGCETVEKKIQKDSSFSERVEKCRTQLFV"
     gene            1196..2317
                     /gene="dnaN"
                     /locus_tag="PK432_01030"
     CDS             1196..2317
                     /gene="dnaN"
                     /locus_tag="PK432_01030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOK59543.1"
                     /translation="MQFSCNQDTLAKYLNIISRVVSTKPGLPILNNVQFEASKGKLIM
                     TATDLEIGINTWIGADVKSEGKITVPARQLTEFVTSIPSDTIDANLDNQTFNISTTNN
                     SASFNTIAADEFPSVTTVTNEKPILKIQKDDLIKAVNRVAFACATDDIKPVLTGVLLE
                     ISGESISFVGTDGLRLSRQIIKLESSAAKEINILVPVKALFELSHIVSEIDEDEDIEM
                     YFIEDRNQVLFRYGDVDLVSRLIDGQFPEYRQIIPTGYKTLCKVSKTDFLNSLKVTNI
                     IAKSVLGNKIILDIDSKTGKITMSATQTDLGSNKSVFEGKIEGDSLRIAFSSRLLADI
                     LSHIDSEDIIFECSETIKPGVFKIVEDPDFVHLVMPMML"
     gene            complement(2262..2981)
                     /locus_tag="PK432_01035"
     CDS             complement(2262..2981)
                     /locus_tag="PK432_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOK59544.1"
                     /translation="MNVLIIEKSKTISHIIKSTLLAYGYNITTDNEDFNSENLLSRGL
                     FEIVILNTNINGKHSTLDILKKIKDVKDNKCKILGICSTGTWKDKVAFLNNGGDDVLS
                     YPFPMQELLARVQSLLRRPQSYIDNKLYIGDVLLDTTNKVVTAKNSDIQLRKKEYQLL
                     EYLVRNKNRTVSRCELLDHVWDYRSYTGSNTIDVHVKRLRDKLPVKGLIETVHGQGYR
                     IKEFKASSALPDEQNQDPQQS"
     gene            3442..>3995
                     /locus_tag="PK432_01040"
     CDS             3442..>3995
                     /locus_tag="PK432_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59545.1"
                     /translation="MAKSFKETIGDEFNDIKEILSGEMKLREREEKLAKLKEDAKKKQ
                     KAEKRKKIAQKEIVLEESDIPTKKVVQNIKLFEWEAPDRYKFNFESKTFWSILALVLV
                     FILYLAILGQYYFMAAVVALVFLIYVAGTNNPVTVKHKITSRGIDTGNKLYEWFMLNS
                     FWFSKKGDQYMLIVETKLRYPKAL"
BASE COUNT         1425 a          535 c          683 g         1352 t
ORIGIN      
        1 gatagagacg ataaggacaa agaaaaatga agagtttaga aacaaatata gacaagtgga
       61 tcttttgatt attgatgaca tacagtttgt tgaaacatac cctaaaacac aagaggagct
      121 attccacact tttaatgctt tgtatcaggc taacaagcag atagtgctgg cttcagatag
      181 agctcctaaa gatattaaaa atattacaga tagattaaga actagatttg aaggtggaat
      241 ggtatgtgat atacaacctc cagaatatga gacaagatta gcaatactgc agttaattgc
      301 catggaaaaa cgggttccaa tatctaaaga tattcttgtt ttaattgcaa aaaatataga
      361 aaacagtgta agagagcttg aaggtgcttt aaacaaagta ataacactgg ttagacttgg
      421 aatgaatcct tcatatgatg atgtagctag gtttttacaa attgacattg agagtaaaag
      481 aaaaagggtt actcctgaaa aagtaataaa tgttgtctct gaagtatttg atgttcctgt
      541 aaatatgata aaaggtcaaa aaagaacaag tgatattgca caggcaagac aagttgtaat
      601 gtatttacta agaacccagc ttgaacttcc actatgtaca attgcaaacg cagttggaag
      661 aaaagatcat acaactgtta tatatggatg tgagacagta gagaagaaaa tacaaaagga
      721 tagctctttt tcagaaagag tagaaaagtg cagaactcag ctgtttgtat aatgtgaata
      781 actgtgtgaa ttacgaaaga aaagtacagg aaaatttaag tggttttgtg gataaaaagg
      841 attgaaataa taagcagatg ctaaatatgc aagaattacc aaatagaata tatcaacaga
      901 aattcacaaa ttattaagag tattccacat tgaagaactc tttaaatttt aattatcata
      961 caacaatatt taattacttt tatatgaatt aaatacacaa aactatcaac ataggaaatt
     1021 aagtaaaaaa tcttttaaac ttaatatttt tatgaaattc tacacatatc catactctct
     1081 actactatta ctactattta tttatatata tttattaaaa cagtagaaaa gagaaaaaat
     1141 atcgagtaga atataggagt aaaactgcca tatataaaat aagagtaaaa gaaaaatgca
     1201 attctcatgt aatcaagaca cactagcaaa atatttaaat attataagta gagtagttag
     1261 tactaaacca ggactcccaa tactaaacaa cgttcagttt gaagcaagca aagggaagtt
     1321 aattatgact gcaactgatt tagagatagg aattaataca tggataggtg ctgatgttaa
     1381 atcagaggga aaaattacag ttcctgcaag gcagttaaca gagtttgtta catctatacc
     1441 atctgatact attgatgcaa atttggataa tcaaactttt aatatttcta ctactaacaa
     1501 ctctgcaagt tttaatacta ttgctgcaga tgagtttcca tctgttacaa cggttacaaa
     1561 tgagaagccc attcttaaaa tacaaaaaga tgatttgatt aaagctgtca acagggttgc
     1621 atttgcttgt gctactgatg atattaagcc agttttgacg ggtgttttac tagaaatttc
     1681 tggagagagt atctcttttg ttggtactga tggtttaaga ctttcaaggc agattattaa
     1741 actagaatca agtgctgcaa aagaaataaa tatcctcgtt cctgtgaaag ccctttttga
     1801 gctttcacat attgtaagtg aaatagatga agatgaggat attgaaatgt actttataga
     1861 agataggaat caggttttgt ttagatatgg ggatgttgat cttgtttcaa gactcattga
     1921 tggtcagttt cctgagtaca gacagataat tcctactgga tacaaaactt tatgtaaggt
     1981 atcaaaaact gatttcttaa actcccttaa agttacaaat ataattgcaa aaagtgtttt
     2041 agggaataaa ataatcctgg atatagattc taaaacagga aagataacaa tgtctgcaac
     2101 tcaaacagat ttaggaagca acaaaagtgt atttgaagga aagattgaag gagattcctt
     2161 aagaatagca tttagttcaa ggcttctggc tgatattcta agtcatatag attctgaaga
     2221 tattatattt gaatgttctg aaactattaa accaggtgta tttaagattg ttgaggatcc
     2281 tgattttgtt catctggtaa tgccgatgat gctttaaatt ctttgattct gtaaccttgc
     2341 ccatgtacag tttcaattag tccctttaca gggagtttat ctcttaatct ctttacatga
     2401 acatctattg tattagagcc ggtatagctt ctgtaatccc atacatgatc taaaagttcg
     2461 catctgctta ctgttctatt tttgtttcta acaagatact ctaatagttg atattctttc
     2521 tttctaagct gtatatcgct atttttagca gtaactactt tattagtagt atcaagtaaa
     2581 acatctccga tatacaattt attgtctata tagctttgag gtcttctaag taaagactga
     2641 actcttgcaa gaagctcttg catagggaaa gggtaggaga gtacatcatc tcccccatta
     2701 tttaagaaag caaccttatc tttccatgtt ccagtactgc atataccaag aatcttgcat
     2761 ttattatctt ttacatcctt aatctttttt aaaatatcta atgtgctatg ctttccattt
     2821 atatttgtat ttagtattac aatttcaaac aatcctctac ttaaaaggtt ttcactatta
     2881 aaatcctcat tatctgttgt gatattataa ccatatgcca gaagggtact ttttatgata
     2941 tgtgaaattg tcttagattt ctctataatt aagacattca tatttatctt tacatttaaa
     3001 aatttaattt aagagaatat tacacaggat aatggattca aaaacttata gttcaaaaaa
     3061 tcttaaattt atctttgtta ctttattttt attaatccta tctgttgcaa ttttgttata
     3121 tttattttct ctctcaagtt ttaagccaat aaacgagaat ggggtatata attggttaaa
     3181 catctcaaca gtagctcttc tttcttttgt aattttgtgc tgtatatcag tacttctctc
     3241 atactttgtt cttaccttta ttttaaagag acaagattgc agggtattaa ggctgtggtg
     3301 tgttaagttt gggctgatac ttacgattgg agtatttttt gtaattcttc ttaacttctt
     3361 tcatatacta gatatatatt ggggtttagg tattcttttt cttgttatct tctcgttctt
     3421 tgttatatga tataactaga gatggcaaaa agctttaaag aaacaattgg agatgagttt
     3481 aatgatataa aagaaattct ttctggagaa atgaaattaa gggagagaga agaaaaactc
     3541 gcaaagctta aagaagatgc aaagaagaaa caaaaggcgg agaagaggaa aaagattgct
     3601 caaaaagaaa ttgttttaga agagagtgat attcctacta aaaaagttgt acagaatatt
     3661 aaactgtttg aatgggaggc tcctgatagg tataagttta attttgaatc aaagaccttt
     3721 tggagtatcc tagctcttgt tttagttttt atcctttatc ttgcaatttt aggtcagtac
     3781 tattttatgg ctgctgttgt tgctttagtt tttctcatat atgtagcggg aacaaataat
     3841 cctgttactg ttaaacacaa gataacgagt aggggaatag atacaggaaa taaactgtat
     3901 gagtggttta tgcttaatag tttttggttc tcaaaaaaag gtgatcaata tatgttaatt
     3961 gttgaaacta agcttagata tccaaaagct ttaat
//
LOCUS       DAOSAB010000032         3990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_15128_length_3990_cov_2.970267, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000032 DAOSAB010000000
VERSION     DAOSAB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15128_length_3990_cov_2.970267"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /gene="rnc"
                     /locus_tag="PK432_01045"
     CDS             <1..604
                     /gene="rnc"
                     /locus_tag="PK432_01045"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOK59546.1"
                     /translation="LNEHKDIKIHNERLEFLGDAVLELIVSDYLFNKFPDRPEGDLTS
                     FRSALVKTESLADTAKKLGVGESLLLSRGEEDTGGRTKDYLLANTFEAIIGAIYIDQG
                     YEKCKEFVYTHLIPKLNDIIKNRSDIDNKTRIQEITQARYKVTPVYEVLNEEGPDHNK
                     KFTIVVKINNKVIGKGVGKSKQKAQEQAALSGIKYLEKEN"
     gene            646..>925
                     /gene="rpsP"
                     /locus_tag="PK432_01050"
     CDS             646..>925
                     /gene="rpsP"
                     /locus_tag="PK432_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOK59547.1"
                     /translation="MLKIRLKRIGRRGQPYYRIVVMENKSPRDGRSIEEIGFYNPRTQ
                     PATFEIDKDAAIKWLSSGAQPSDTIAQYFVKLGLIKPSKKGSVKPNTTK"
     assembly_gap    926..935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1080..1373
                     /locus_tag="PK432_01055"
     CDS             1080..1373
                     /locus_tag="PK432_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOK59548.1"
                     /translation="MKELLEHIVKSIVNHPEDVSIEEKESVDFPGLTILTINVNEEDK
                     GVIIGRKGRTINSIRDIITISAIRNNKRVRVILKDDNKPNREENQEDMLNDEV"
     gene            complement(1374..2063)
                     /locus_tag="PK432_01060"
     CDS             complement(1374..2063)
                     /locus_tag="PK432_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59549.1"
                     /translation="MALVTSQTEKKKKAELQKVVEKRGSKKLTISDLIIPIASSIVLL
                     VLCFAVFIPMVRSAFEYMDEMKEIDQKIEQLDKLNKQLDQLDESQLSDDVLTSRTVVP
                     NTLLVSDLVYYIDNLAKSLNLQISKLSSADSLNSVSGPLGYEGEYTNVIDFLDKAQNV
                     SPYMIRLENVKLSGKNKNDGKDNWSIDMIVSGYYISDSKEEPSIYSNFQPYTEHSDVL
                     EIFREKAKSTL"
     gene            complement(2066..2776)
                     /locus_tag="PK432_01065"
     CDS             complement(2066..2776)
                     /locus_tag="PK432_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59550.1"
                     /translation="MNNNLQNQTVSDINNNSIPSQQVPPSAGQVNMQPPVQGSPTLAE
                     PIAAQIKEETEGVNLIPSRTKEEEIQVKKKTTLSVGSLLAIIILALVSIGIVGFNIIT
                     KNQLSDKKKELANMENIVNQKADKIIANNVILDRINLYKKVKANSFSHKKVITFLREM
                     AGKIQGITYDSMEISEDLSFKISGDAPNLEQLSRMWYLFGVNENIETIKLKSFSKTET
                     GATFSFDGKLVLDNFKAD"
     gene            complement(2769..3797)
                     /gene="pilM"
                     /locus_tag="PK432_01070"
     CDS             complement(2769..3797)
                     /gene="pilM"
                     /locus_tag="PK432_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOK59551.1"
                     /translation="MAKLPDHVGVDFGTHTVKAVELKNILSTPELVNLGMQITPKGVI
                     NSEERQDQKKLAEALKKLYDSAKIKNPYVVMALPEFSVFTRFLEFPGVKEEELKNAVY
                     YEAKQYLPVSVDEVQMSYVTIGFNEEKNAPRVLLVAAPKKIISIYMNVASFANLDLIA
                     IETESVAMGRAMYRAMKKKDCVTLDFGANSTDMCIMTNGALVFSQSIPIGSDSLTQAI
                     VNKFNFDYNRAEEFKRAYGLIPNALEGKIYSALLPIVESIMIEVRRGIEFYKNKTMSP
                     APMEYLLNGDGALLPGLTEYVSKSLGVSAQLANPWLNISIDNRFKNIISKTGGSYSIA
                     VGLALKDE"
BASE COUNT         1370 a          641 c          611 g         1358 t
ORIGIN      
        1 cttaaatgag cataaagata ttaagattca caatgaaaga ttagagtttc ttggagatgc
       61 agtattagag ctaattgttt ctgattattt gtttaataaa tttcctgata gaccagaggg
      121 tgatcttaca agttttagat ctgcattagt taaaacagaa agtttagcag atacagctaa
      181 aaagctggga gtaggagaaa gccttttgct ctctaggggt gaagaagata caggaggaag
      241 aacaaaagac tatctattag ctaatacttt tgaagcaatt attggagcta tatatattga
      301 tcaaggatat gagaaatgta aagagtttgt atacacacat ttgataccta aacttaatga
      361 tatcattaag aataggtctg acattgataa taaaactaga attcaagaaa ttactcaggc
      421 tagatataag gttacacctg tgtatgaagt actcaatgaa gagggacctg atcataataa
      481 aaaatttact atagtagtta agattaataa taaagtaata ggtaaaggag tagggaagag
      541 taaacaaaaa gcacaagagc aagcagctct tagtggcatt aaatatcttg aaaaggaaaa
      601 ttgattactt gaatattaat atatcttctg ttaaaatatt ctgctatgtt aaaaataaga
      661 ttaaaaagaa taggaagaag aggacaacca tactacagaa tagttgttat ggaaaataag
      721 tctccaagag atgggaggag tatagaagag attggttttt ataatccaag aacacagcct
      781 gctacatttg aaatagataa agatgctgct attaaatggc tttcttctgg agcacagcct
      841 tcagatacta tagctcaata ttttgtaaaa cttggtttaa ttaaaccttc taagaaaggt
      901 tctgttaagc ctaatacaac aaagannnnn nnnnnggaaa taatcagtat catggtgaca
      961 caggaggaaa gaaggaaaag aaaaaagaat agacttcact ttactcttga ttttctggta
     1021 atattatttc aataggggta taatttaccc agtaagatat tagtttagca aacattaaga
     1081 tgaaagaatt actagaacat attgttaaat ctatagttaa tcacccagaa gatgtctcta
     1141 tcgaagagaa agagagtgta gactttcctg gcttaacaat attaacaata aatgtaaatg
     1201 aggaagacaa aggtgtaata ataggaagaa aagggaggac aattaattca atcagagata
     1261 ttatcacaat tagtgcaata agaaataaca aaagagtaag agtaatactt aaggatgata
     1321 ataaaccaaa cagagaagaa aatcaggaag atatgctcaa cgacgaagtc taattaaaga
     1381 gtactctttg ctttttccct aaatatttca agaacatcgc tatgctctgt atatggctgg
     1441 aaattgctgt aaatacttgg ttcttcttta ctatcagata tataatatcc acttactatc
     1501 atatcaatag accaattatc ttttccatcg tttttatttt ttcctgataa ctttacattt
     1561 tctagtctta tcatatatgg agatacattt tgtgctttat ccaaaaaatc tattacatta
     1621 gtatattctc cttcatatcc aagaggacct gatactgaat ttaaagaatc tgcagaagag
     1681 agcttagaaa tctggagatt taaagatttt gctaaattat ctatgtaata tactaagtct
     1741 gaaacaagca aagtgttagg aacaacagtt cttgaagtca gtacatcatc acttaattga
     1801 ctctcatcta attgatcaag ctgcttattt agtttatcca actgttcaat cttttgatca
     1861 atctctttca tttcatccat atattcaaag gctgatctta ccattgggat aaatactgca
     1921 aaacaaagga caagaagtac tattgaagat gcaattggga ttataaggtc agagatagtt
     1981 aacttcttgc tccctctctt ttcaactact ttttgtaact ctgctttttt ctttttttca
     2041 gtttgtgaag taacaagtgc cataattaat cagctttaaa gttatctaaa actaatttac
     2101 catcaaaaga aaaagtcgct cctgtttctg ttttagaaaa tgactttagt tttattgtct
     2161 caatattttc atttacacca aaaagatacc acatacgaga aagctgttct aaattgggtg
     2221 catctccaga tatcttaaaa gataaatctt ctgatatctc catactatca tatgtaatac
     2281 cttgtatttt acccgccatt tctcttagaa aggttataac ctttttatgt gaaaagctat
     2341 tagctttaac ttttttatac aggttaattc tatctaaaat aacattattc gcaattattt
     2401 tgtcagcttt ttgattaaca atattctcca tattagcaag ctctttcttt ttatcactaa
     2461 gctgattttt agtaataatg ttaaatccta ctataccaat agaaaccaag gctaagataa
     2521 taatagccaa aagggaacca acacttaatg ttgttttttt cttaacttga atctcctcct
     2581 cttttgtacg agagggaata agattaacac cttctgtttc ttccttaatt tgtgcagcaa
     2641 taggttctgc taaggttggg gatccctgta ctggcggctg catattaact tgaccagctg
     2701 atggaggtac ctgttgacta ggaatactat tattattaat atcactaact gtttgatttt
     2761 gtaaattatt attcatcttt tagtgccaaa cctactgcaa tagagtagga acctcctgtt
     2821 tttgaaatta tatttttaaa tctattatca attgatatat ttaaccatgg atttgctaat
     2881 tgtgcactaa ctcctagcga ttttgataca tactctgtta accctggaag aagagcacca
     2941 tctccattaa gtagatactc cataggagca ggactcattg ttttattttt ataaaattca
     3001 atacctcttc taacttcaat cattatagat tcaacaatag ggagtaatgc actatatatt
     3061 tttccttcta aggcattagg tataaggcca tatgctcttt taaattcttc cgccctgtta
     3121 taatcaaaat tgaatttatt tactattgct tgtgttagag aatctgatcc aataggaata
     3181 ctttgagaaa agaccagtgc tccatttgtc ataatacaca tatctgtact atttgcacca
     3241 aaatctaatg taacacaatc tttcttcttc atagctctat acattgctct tcccatagca
     3301 actgactctg tttcaatcgc aataagatca aggttagcaa aactggctac attcatatat
     3361 atactaatta ttttctttgg agctgcaaca agtagaaccc tcggggcatt cttttcctca
     3421 ttaaaaccaa tagtcacata actcatctga acttcatcaa cagatacagg aagatactgt
     3481 ttagcttcat aatatacagc attttttaat tcctcttcct ttactcctgg gaattctaaa
     3541 aatcttgtaa atacagaaaa ttctggtaat gccattacca catatggatt tttaatctta
     3601 gcactatcat aaagcttttt taatgcttct gccaattttt tctggtcttg tctttcttcg
     3661 ctgttaataa ctccttttgg tgtaatctgc atcccgagat ttacaagctc aggtgttgag
     3721 agaatattct ttaattctac agcttttaca gtatgtgttc caaaatctac acccacatga
     3781 tctggtaatt ttgccatagc tttggacagt ttaaattatc actaatatat aatagcagaa
     3841 attggataat gttcaaaatc ctatatgtta tggcttaaca ccaatctcaa cagcttttac
     3901 tgtacttttt cttccaaaga acttttctgc tattactcca tatttagggt gataatctat
     3961 tactaatact ggatatgtaa gcttttcttc
//
LOCUS       DAOSAB010000033         3982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_15177_length_3982_cov_2.619557, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000033 DAOSAB010000000
VERSION     DAOSAB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15177_length_3982_cov_2.619557"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="PK432_01075"
     CDS             <1..1367
                     /locus_tag="PK432_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59552.1"
                     /translation="SYAVEDVPQTITPDWEGEKKFHNIVNKADETEDNSQTSLGQQNV
                     TTLWTGVLYLGGEDLLDEETKAKLEAANVPYDLRRGVIGMIDDASMAVYASYPLVNVP
                     DHLAQQWVPGYKDSTTALYAEDGTWSGYSELKAAGIQPLWTNVLNLAYLFFVIVMIIA
                     GFMIMFRHKIGGQAMVTLGNVLPNVIIALVVATFSFAIAGLIIDFGGLIIGLIVSVFD
                     LSDTATSIAGFGKLFSNVFTGGVKMATLISGIGGALGLGGILGGAKLGLFTAVTNPVA
                     WAVGAAVGAIGLLFTLIVLGIIAFGAIKVLITLFKAYFQLLLSVILGPLQITLGAIPG
                     NNAAIMNWMKGVLRNVLVFPVVFFIVNLPNALIKNGAEMRLRLPAKLVYETEGYDASG
                     PNVTGGLVLFVLKIFVLFFAAQAPKFIESFLPPSSNKGLAAGFDSVRGSLQKVPLIGG
                     MFGK"
     gene            1391..>3982
                     /locus_tag="PK432_01080"
     CDS             1391..>3982
                     /locus_tag="PK432_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59553.1"
                     /translation="MPNKGKNDNYDAVGGIIDFILAESEKPPEKRRPVKPPTGVSASS
                     ALTDAIFASLENPGAFISNTLIDEFNDALDVKIAQVDFFGGRGNLKLSANNLGNWLRD
                     PGGQIQKAMDRAEADRKAARARMIGEAIDDFLTTAWAHKYGNLEVKKIALANASANEK
                     AEAYKVARAVGQSVAPSGDRDFMANRTVELLGQKTFGNRWFTMNEQEKLEFSSKLGAK
                     SNRKEIESYLARRYGKTEAENFDHLLREKGYEEKINVFDPDLYRELENNYLMEKIYSL
                     KNAPEGSEEEKQRKIYEKTKMLVNLRAKHEIGMLKDMLKEGNLSDDKRRKLERQINDA
                     EIAIREVGGVYNLATGIGRWEGYLNSIKMLGGATGTNVVGSILNGDFFKADKNKFAPV
                     KKVPLEEMPGVEIYVAKKDRNKVQNAYNEMGEALYYATPRSLFRTFFYNGELFARGLY
                     KNNEQLKELLNGLGATGVLGEVIGADSVIDNIYSLDGKKLNEYISKTLEKIQTQLASG
                     TLSVKDFEKIEKLLKQSKNLKNLTKIFSFPARTKKMIEDKIKEVFDVPLKKARQRIYD
                     AIMKNEALRNWITKTLGESMLKEFVAKGGLRNLIKPLVSAVAGALGVALTPLASIAIT
                     IGVNIAMDLVGKIAKVFVQIMLVAIVGLIAAIVVLGGSVGSWKKWNRKTFSYNYVVPD
                     TVNQCPAYGGQNYNIPPPDGGDGWVPGPGLPPDWEGAKGVYEIFKRARDYVSREYGTV
                     NTELVLLDCNSGSDPTGMCAAIDWAWCYSASSIYCKVDKLTSASPQYVFALSVHELIH
                     QLQRGCTTDIREWGADYLSSNGGGYVFSTPSGCKRATEINTSSCSRQEAVDAALCRNT
                     NTDCFRQI"
BASE COUNT         1305 a          537 c          934 g         1206 t
ORIGIN      
        1 attcttatgc agtggaagat gtcccgcaga ctattactcc tgactgggag ggcgaaaaga
       61 aattccataa tatagttaat aaggcagatg agacagaaga taactctcaa acaagtttgg
      121 gacagcaaaa tgtaacaaca ctctggacag gtgtacttta tttaggtgga gaggaccttc
      181 tggatgaaga aactaaagca aagctagaag ctgcaaatgt tccatatgac ttaagaagag
      241 gagtaatagg gatgatagat gatgcttcaa tggcagttta tgcaagctat cctctagtga
      301 atgttcctga tcatttagca cagcaatggg ttccagggta taaagatagc acaactgcac
      361 tgtatgctga ggatggtaca tggtcagggt atagtgagct taaagctgcg ggtatacagc
      421 cattgtggac taatgtactt aaccttgcat atttattctt tgttattgta atgataatag
      481 cagggtttat gattatgttt aggcataaga taggaggtca ggctatggtt acattgggta
      541 atgttttacc aaatgtaata attgctttag ttgttgcaac atttagtttc gcaatagcag
      601 gacttattat agattttggt ggattgataa taggtttgat agtaagtgtt tttgatcttt
      661 ctgatactgc cacttcaata gctggatttg ggaaattatt ctctaatgtg tttactggag
      721 gtgttaagat ggccacactt attagtggaa ttggtggtgc acttggtcta ggtggaattc
      781 tgggtggtgc aaaattaggt ctttttactg cagttacaaa tccagttgct tgggcagtag
      841 gtgccgctgt tggcgctata ggtttacttt ttactttgat tgtcttagga ataatagcat
      901 ttggggcaat taaagtatta attacactat ttaaagcata ttttcagctt ttactttcag
      961 ttattttagg acccctgcaa attacattag gagctattcc aggtaataat gcagctatta
     1021 tgaattggat gaaaggtgtt ttgagaaatg ttttagtatt tcctgttgtg ttttttatag
     1081 ttaatttgcc caatgcactt ataaaaaatg gtgctgaaat gagattgcga ttacctgcaa
     1141 agcttgtata tgaaactgaa ggatacgatg cgagtggtcc aaatgttaca ggtggccttg
     1201 ttcttttcgt cttaaagatt tttgtattat tctttgctgc tcaagctcca aaatttatag
     1261 aatcattctt acctcctagc tctaacaagg ggcttgctgc aggatttgat tctgtaagag
     1321 gcagcctaca aaaggtacca ttaattggtg gtatgtttgg taaataatta gtcataatat
     1381 aaggagaaac atgcctaata aaggtaaaaa tgacaattat gatgcagtag ggggaattat
     1441 tgattttata cttgctgaat cagagaaacc cccagagaaa agaagacctg ttaaaccgcc
     1501 tacaggggtt tctgcttcta gtgcacttac tgatgctata tttgcttctt tggaaaatcc
     1561 aggtgccttt atttcaaata ctttaattga tgagtttaat gacgccttag atgttaagat
     1621 tgcacaggtt gatttttttg gtggtagagg aaatctgaag ctttctgcaa ataatttggg
     1681 taattggcta agagatcctg gcggtcaaat tcaaaaagca atggatagag cagaggcaga
     1741 tagaaaagct gcaagggctc gtatgattgg tgaggctata gatgatttct taactacagc
     1801 atgggctcat aaatatggaa atttagaagt taaaaagata gccttggcaa atgcttctgc
     1861 aaatgaaaaa gcagaagcat acaaagttgc aagagcagta ggacagagtg ttgccccatc
     1921 tggagatagg gattttatgg ctaatagaac agtagagtta cttgggcaaa aaacgtttgg
     1981 gaatcgctgg tttactatga atgagcagga gaagttagag ttctcaagta aacttggagc
     2041 aaaaagtaat cgtaaagaaa tagaatcata tcttgctcgt aggtatggca aaacagaagc
     2101 tgagaatttt gatcatctcc taagggaaaa aggctatgaa gagaagataa atgtatttga
     2161 tcccgaccta tatagagaac ttgaaaataa ttatcttatg gaaaagatat attctttaaa
     2221 gaatgcacca gagggctctg aggaagaaaa gcaaaggaag atatatgaaa agacgaagat
     2281 gcttgtcaat cttagagcaa agcatgaaat tggtatgtta aaggatatgc ttaaagaagg
     2341 gaatctttct gatgataaaa gaagaaaatt agaacgtcaa ataaatgacg cggaaatagc
     2401 aatcagagaa gttggagggg tatacaactt ggcgacaggg attgggagat gggaaggata
     2461 tcttaattca ataaagatgc ttggtggtgc aacgggaact aatgttgttg ggtcaattct
     2521 caatggagac tttttcaaag cagataagaa caaatttgct cctgtaaaga aagttccctt
     2581 agaagaaatg ccaggagtgg aaatatatgt tgctaagaaa gatagaaaca aagtacagaa
     2641 tgcatataac gaaatgggag aagctcttta ctatgcaaca ccaagatcac tttttaggac
     2701 atttttttac aatggagagt tatttgcaag aggtttgtat aaaaataatg agcagttaaa
     2761 agaacttttg aatggacttg gtgcaactgg agtattagga gaagttattg gtgctgatag
     2821 tgtgattgat aatatatatt ctttagatgg aaagaaactt aatgagtata tcagtaaaac
     2881 attagaaaaa atacagacac aacttgctag tgggactctt tcagtaaaag attttgaaaa
     2941 aattgagaaa ctgctaaaac aatccaagaa tcttaagaat ttaacaaaaa tctttagctt
     3001 tcctgctagg acaaagaaga tgatagagga taagattaag gaggtctttg atgtaccatt
     3061 aaagaaggct agacaaagga tttatgatgc cattatgaag aatgaggctc ttaggaactg
     3121 gattactaag actttaggag agagcatgtt aaaagagttt gttgcaaaag gtggtttacg
     3181 aaacttaatt aaacctcttg taagtgctgt ggctggtgca cttggggttg ctcttactcc
     3241 acttgcatcg attgctataa caattggtgt aaatatagct atggatttgg tagggaaaat
     3301 tgcaaaggtt tttgtacaaa taatgttagt tgctattgta ggtcttattg cagcaattgt
     3361 tgtattaggt ggtagtgttg ggtcatggaa gaaatggaat agaaagacat tctcatacaa
     3421 ttatgttgtc ccagatactg ttaatcaatg tcctgcatat ggtggacaaa actacaatat
     3481 tcctcctcct gatggaggtg atggctgggt tcctggacca ggcttaccac ctgattggga
     3541 aggggcaaaa ggagtttatg agatttttaa aagggcgaga gattatgtat caagagaata
     3601 tggtactgta aatactgagc ttgtactgct tgattgtaac agtgggagtg atccaacagg
     3661 aatgtgtgca gcaatagact gggcttggtg ttatagtgca tcatcaatat attgtaaggt
     3721 ggataagcta acgagtgctt caccacagta tgtctttgcg ctttctgtac atgaactaat
     3781 acatcaatta caaagaggct gtactacaga tattagagaa tggggagcag attatctatc
     3841 ttctaatgga ggtggatatg tttttagtac accaagtgga tgtaaaagag ctactgagat
     3901 taatacatca tcatgttctc ggcaggaagc tgttgatgcc gcattgtgta gaaatacaaa
     3961 tacagattgt tttaggcaga ta
//
LOCUS       DAOSAB010000034         3957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_15325_length_3957_cov_16.059200, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000034 DAOSAB010000000
VERSION     DAOSAB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15325_length_3957_cov_16.059200"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..3665)
                     /locus_tag="PK432_01085"
     CDS             complement(93..3665)
                     /locus_tag="PK432_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59554.1"
                     /translation="MASCRITESKNERLVELVNQFGQQEGLRRFLAEQAGYDFDVAET
                     PPEDDLQFPITNKLAQALEQQIKLKYKIKNTLIFEARAARQAGRTSKATELSGKIQAV
                     QTQITSLENQKRSLTGEIRLQTLVDISKIHLNWLDQLLSRETVMPSEIIEAYRLLDIW
                     SNIDDILFDLTDDNTEIHPVIWAKIVSIKNDIISKKYDKKLFQLASELLSSTAEYDSV
                     ADFLQDMYRLKDISTANAYANFVADMGVKLLSKLDRLNKDAIARTQGELAKWLKRLEP
                     IKELSKSGRLDIFWQRDENGKLTGGLVNRYSQEWYNNFFGRVKTFYQILTSKASLDKK
                     LKTIKEFGIWLNTNTHYVDPRFFYVKDYVNHAGQNFAQFRHALELDLGKEEADALIQR
                     AKDLYEQYLTDLQNYTDYITAQFEGEEDAEEKIKTKITKWKQYNNPAVWIDQLNGKTT
                     EESNKYAIKQVHLNRYVYYTPKKNINGNPSKFYDEAFSAIMADPELSKAYNLIHEFMT
                     EMLSYIPPYINLQSNFLPRVRKKFVEDLTVNGIFSALANAPMDFLDSITTSIDEVNIE
                     DAQPYGEKFTKNIPVRYIGEFDEKEMSTDFSRILPLFAATALNYKWKSRIQDTIELTH
                     KFLGDIAQSATRKEMTSDDLNNLRNALEWFMDTQLYQEKRLDEGVTNIKTFRGDKRVF
                     IDIVEKDPRIVESIKKEYAFLLKDNPPERAAQILVENWGDRVVILNAKQKYEKLLQAA
                     DDLQRKFEDGEISEQVFEKSMQNIEQEAQQLGRNVVVSKLLDKLMTHNQAMAFWFNPF
                     SAFNNFAFGVVSNLIWAADGTDFKVKDSIKSFWIMLRSAFNLKDGKLDKVAQLMAKFN
                     MMAERLEYETSTIQNETMRKLKDIPYVLLRKGDYFIKGMTMVAAMMNKQIEVTVNGKK
                     QTISLWEAFDEHGNWNTELYGENPDWNGDFSNPEENKEFVNFRLYVQALSTKLHGNFD
                     PSTTPMYKKYVFLRMLGQFRASWVIEGVKDRFQAKRYDEYLGRYVEGRYVTAFHLGFK
                     KSLKTLYKLTLDLMGIEQDWKYLPVRDRKLIIANMRRNLMEIYLYMAMLATYLLIKAS
                     IDDDDDEDKWGTITALNTLNRVMQDTTFYLSPKTFIEIVKDPIPLLSLAIRAGRGVGA
                     AMDLIMDSGELTDRQIELNWRKITSNFYFINQYNKFINMSSNLY"
     gene            complement(3667..>3957)
                     /locus_tag="PK432_01090"
     CDS             complement(3667..>3957)
                     /locus_tag="PK432_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59555.1"
                     /translation="FYENTITNILKKQGYNPVLITDEYGNTWNEVTIDENRDNQNIVF
                     QKTPKESTSKPDRFHTTDLGTTVSKFMQSLERDQRKILREMIRNGEIKFKCS"
BASE COUNT         1139 a          786 c          572 g         1460 t
ORIGIN      
        1 aaagtattat tcaatggtaa aaagggtaga gggaaataaa aacccctcta cccccctttt
       61 gctgtaactg gttccttttg tttgccttgt tattagtata aattagaact catatttata
      121 aacttattat actgattaat gaaatagaag ttacttgtaa ttttcctcca gttcaattct
      181 atctgcctat cagttagttc tcctgaatcc attattaggt ccatagctgc tcctacacct
      241 ctaccagcac gtattgctaa acttagtagt ggtattgggt ctttaacaat ctctataaaa
      301 gttttagggc ttagataaaa tgtggtatcc tgcatcacac gatttaatgt attaagagct
      361 gtaattgttc cccatttatc ctcatcatca tcatcatcta tagaagcctt aattaacagg
      421 tatgtggcaa gcattgccat gtataggtat atttccatta ggtttctacg catatttgct
      481 attataagtt tcctatccct aactggcaaa tacttccagt cctgttctat acccatcaaa
      541 tccaaagtca acttatacaa agttttcagg gactttttaa aaccaaggtg aaaggctgtt
      601 acatacctac cctcaacata ccttcctaaa tactcatcat accttttagc ttgaaacctg
      661 tcctttacac cctcaataac ccaacttgct ctaaactgtc caagcattct taagaataca
      721 tacttcttat acattggggt tgttgatgga tcaaagttac catgaagttt agtggatagg
      781 gcctgcacgt aaagtctaaa gtttacaaac tctttatttt cttctgggtt actaaagtct
      841 ccattccaat caggattttc cccgtacaac tctgtattcc agttaccatg ttcatcaaat
      901 gcttcccaaa gggatattgt ttgttttttc ccatttactg taacctcaat ctgtttgttc
      961 atcatggctg ctaccatggt cattccctta atgaagtagt ctccctttct aagtaaaaca
     1021 tatggtatgt ccttcaactt tctcatagtt tcattttgga tggtagaagt ttcatattca
     1081 agcctttctg ccatcatgtt gaatttagcc atcaattgtg ctaccttatc aagtttacca
     1141 tccttgagat taaaagcaga tcttagcatt atccagaagg atttgatgga atccttaact
     1201 ttaaagtccg ttccatcagc agcccatata agatttgata ccactccaaa ggcaaagttg
     1261 ttaaaagcag aaaatggatt aaaccagaaa gccattgctt gattgtgagt catcagttta
     1321 tctaacaact tagaaacaac cacatttctt cctagttgtt gagcctcctg ttctatattc
     1381 tgcattgatt tttcaaatac ttgctcagat atttctccat cctcaaattt tctttgtaag
     1441 tcatcagcag cctgcagtag tttttcgtac ttctgtttag cattcagtat gactacccta
     1501 tctccccagt tttctactaa tatttgagct gccctttcag gtgggttatc ttttagaagg
     1561 aatgcatact cctttttaat agattctact attcttgggt ctttttctac aatatctata
     1621 aatactcttt tgtcccctct aaaagttttt atattggtta ctccttcatc aagacgtttc
     1681 tcctggtaca actgggtatc cataaaccat tcaagtgcat tccttaggtt atttaagtca
     1741 tctgatgtca tttcttttcg tgtagcactc tgagcaatat cccctaaaaa tttatgtgtt
     1801 agttctatag tatcttgaat tcttgatttc catttataat ttagagcagt tgctgcaaaa
     1861 agaggtagta tcctagaaaa atctgtggac atttcttttt catcaaattc tccaatatac
     1921 ctaacaggaa tgtttttagt aaatttttcc ccatatggtt gtgcatcttc aatgtttacc
     1981 tcatcaatgg aggtagtaat actatctaag aaatccattg gagcatttgc cagtgcagaa
     2041 aatataccat taactgttaa atcctctaca aattttttac gaactctagg taaaaagtta
     2101 gattgaagat ttatataggg gggaatgtat gacagcattt ctgtcatgaa ttcatgaata
     2161 agattatatg ctttactaag ttctggatcc gccataattg cagaaaaagc ctcatcatag
     2221 aacttagatg gattaccatt tatatttttc tttggagtgt agtacacata cctatttaaa
     2281 tgcacctgct taatagcata cttattactt tcttcagtag tcttaccatt taactggtct
     2341 atccaaacag caggattatt atactgcttc catttagtta ttttggtttt tattttttcc
     2401 tctgcatcct cttctccctc aaattgagca gtaatataat cggtataatt ctgtaaatcg
     2461 gttaagtact gctcatataa atcttttgct cgttgtatca aagcatctgc ttcttcttta
     2521 cctaaatcca attcaagagc atgtctaaat tgagcaaaat tctgtcctgc atggtttaca
     2581 taatctttaa catagaaaaa tctagggtca acatagtgag tattggtatt aagccaaatt
     2641 ccaaattcct tgatggtttt taatttctta tctagggagg ctttagatgt tagtatttgg
     2701 taaaaggttt ttactcttcc aaagaaatta ttataccatt cctgagaata cctattaacc
     2761 aatccaccag tcaactttcc attctcatct ctctgccaga atatatccag cctaccactt
     2821 ttgcttagtt cttttattgg ttcaagcctc ttcaaccact tagcaagttc tccctgtgtt
     2881 cttgcaatag catccttatt tagcctatca agtttagaga gtagtttaac tcccatatct
     2941 gctacaaagt tagcataggc attagcagta gatatgtcct ttaatctata catatcctgc
     3001 agaaagtctg ctacactatc atactcagca gttgaagaga gtaactcaga agctaactga
     3061 aaaagttttt tatcatattt cttagaaatg atatcattct taatggaaac tatttttgcc
     3121 caaataactg gatgtatttc tgtgttgtca tctgttaaat caaaaagtat atcatctata
     3181 ttactccaaa tatctaacaa tctgtacgcc tcaataattt ctgagggcat aacagtttcc
     3241 ctgctaagca gttggtccaa ccaattcaaa tgaatttttg aaatatctac taaggtttgt
     3301 aatctaattt ctccagttag actccttttt tggttctcca aagaggttat ctgtgtctga
     3361 acagcctgaa ttttaccact caactcagta gctttagaag ttctaccagc ctgtcttgca
     3421 gctcgtgcct caaaaattaa ggtattctta attttatact tgagctttat ttgctgttct
     3481 aaggcctgtg ctagcttatt agtaattgga aactgtaaat catcttctgg aggtgtttca
     3541 gctacatcaa aatcatatcc agcctgttca gcaagaaatc ttcttaatcc ttcttgctgt
     3601 ccgaactgat taacaagttc aacaagcctt tcatttttac tttcagtaat tctacaacta
     3661 gccatattaa ctacatttaa attttatttc accgttacgt atcatttctc ttaaaatctt
     3721 tctttgatct ctctctagag attgcatgaa tttacttaca gtagtaccca agtctgtagt
     3781 gtggaatcta tcaggtttac tggtggattc tttaggtgtt ttttgaaata caatattttg
     3841 attatctcta ttttcatcta ttgttacttc attccaggta ttaccatact catcggtaat
     3901 taatacagga ttgtatcctt gttttttaag aatattagtt atagtatttt cataaaa
//
LOCUS       DAOSAB010000035         3939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_15406_length_3939_cov_2.747425, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000035 DAOSAB010000000
VERSION     DAOSAB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15406_length_3939_cov_2.747425"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PK432_01095"
     CDS             <1..554
                     /locus_tag="PK432_01095"
                     /inference="COORDINATES: protein motif:HMM:NF025760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM domain-containing protein"
                     /protein_id="HOK59556.1"
                     /translation="RYIVGSKTYGIYAPIILAIAYSYTGLKYGLAITFVVILTSLLSY
                     SVLRRVRMHYITRIAANYTILSMVLVLFFVIVDTFGLGLENMSNIPPLAFICIATLSD
                     FFIRQYTKKSIKTSIVTLLGTILVAIAGWFVITRDIISNYILNNLWIIPLLTLFNILL
                     GTFKGLRIKDLFRFRFTTRENEK"
     gene            544..2022
                     /locus_tag="PK432_01100"
     CDS             544..2022
                     /locus_tag="PK432_01100"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HOK59557.1"
                     /translation="MKSNNPIKLKKEVLGLNRRNQEYVRPFNSPSAKAIADNKIYTKR
                     VLKKELIQTPEVYKLIRTKKQLEFLDWDSLPKSFVIKPNAGTGGNGIIVFYGKKKGKY
                     EWIRPNGTIMTQRDIILHIESILEGRFSMGFGNDIAIIEERIKTDPLLKQYSYKGVPD
                     VRIICFNLVPIMAMLRLPTKRSNGTANLHAGAICTGIDIGTGITTYSMHMRPNSLISD
                     TYEVIDSTLDLKENKPLSGIQIPRWNTLLEIAIKCQKVTGLGYVGVDIALDADKGPVV
                     FEVNARPGFGIQVANQAGLRWRLEKVKDVKIKGIKHGIRVAKSLFGGEVEESIEAISG
                     RKVLNIVERIYVYDKKADRSKLEKYKDSKRELVKAYMNTGVLTSRIDSKLANRIGYFN
                     DHKQFEKYGIPKKFESLKDAQDFIDRNEKEIIKNSSIKRLAKIVENGEIKVRPVFDIP
                     IKIEDQIKITEFISTEISDISQPVIIGKTDLTGYLIDTTNTF"
     gene            2037..3011
                     /locus_tag="PK432_01105"
     CDS             2037..3011
                     /locus_tag="PK432_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59558.1"
                     /translation="MTKSYEQYLKEAGNVHLAYYLEAADELNIKYTVLVHGLLAKFET
                     ENKHWYIINTATPLTNTPSTTIAKRKNLTNKILSSFSIPVPKQVTLNNKDDALRFFKE
                     YKNIVIKPSQQLGGIGVSLLPQNKKQVVNAFNSALRVSKGSHKVIGEEFVEGDNYRLL
                     VLGNRVIGIVRRKPAFVVGNGKDSIQTLIKKKNVERKKNLMKPIRIDNEVHQRLENKG
                     ISLKYIPKENEEVILRYNCNLTTGGTTQECASQTHQYYKDIAVNAVHAIGAKFGGVDI
                     ITQDITKPTKCAVNEINYNPGLRLHYKVDEGEVVRVAVPIQEYISKMI"
     gene            3019..>3939
                     /locus_tag="PK432_01110"
     CDS             3019..>3939
                     /locus_tag="PK432_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59559.1"
                     /translation="MIQTIQGINLESETSKVKIPLNGNNIGDITELLDTISSLHTIFI
                     NEYTISPSLLTISSKLPFLWRESAETLQKLSKQEITLNQAKELIINVLINKRIKSMST
                     ISLLESCMKKNIEITPSIIESAEMITEKEEIGKGANRYFTLGCGRGSQITCSASSTKD
                     AYIAQKLQRDKWITNTFLQRLGMTLPKWELLPNKSYIKKVWDKYEKPVVIKPTGLTAG
                     KGVTVGIDTIQKAQDAFDYAKAAIDTRVRQAWQEKIMIQEQVYGEDYRLLVINGKLQI
                     ATKRIPAFVTGDGSKSIKELIEQENSDPRRD"
BASE COUNT         1605 a          550 c          688 g         1096 t
ORIGIN      
        1 caagatatat agtaggaagt aagacatatg gaatatatgc accaataata ttagctatag
       61 catattcata tactggatta aaatatggtt tagcaattac ttttgttgtt atactaactt
      121 cccttctttc atactctgta ttaagaagag taagaatgca ctatataaca agaatagctg
      181 caaattatac tattttatca atggtattag ttctcttctt tgtaatagta gacacctttg
      241 gcttaggttt agaaaatatg tcaaacattc cacctcttgc ctttatatgt atagctacac
      301 tatcagattt ctttataaga caatatacta agaagtcaat aaaaacgtct atcgttacac
      361 tactaggaac aatattagtt gcaatcgcag gatggtttgt aataacgaga gatattatct
      421 ccaactacat attaaacaat ctatggatta ttcctcttct aacactcttt aatattctac
      481 ttggtacatt taaaggatta agaataaagg atctctttag atttagattt actacaaggg
      541 agaatgaaaa gtaataatcc aataaaatta aagaaagaag ttcttggtct taatagaaga
      601 aatcaagaat atgtaagacc atttaactct ccatctgcta aagctatcgc agataataaa
      661 atatatacta aaagagtact caaaaaagag ctcatacaaa caccagaagt atataagtta
      721 ataagaacta aaaaacagct tgaattcctt gattgggata gtcttccaaa aagctttgtg
      781 ataaagccaa atgcaggaac aggaggaaat ggaataattg tattttatgg taagaaaaaa
      841 ggtaaatatg agtggataag acctaatgga acaataatga cacaaaggga tatcatactt
      901 catatagaga gcatattaga aggaagattc tctatgggtt ttggaaatga tattgcaata
      961 atagaggaaa gaataaagac agatccactg cttaagcagt attcatataa gggagttcca
     1021 gatgtaagaa taatctgctt taatcttgtt cccattatgg caatgctgcg attacctaca
     1081 aaaagatcaa atggtactgc gaatcttcat gcaggtgcaa tatgtacagg tatagacata
     1141 ggaacaggaa taactaccta ctctatgcat atgagaccta attctttaat tagtgataca
     1201 tacgaggtca tagattcaac attagattta aaagaaaata aacctttaag tggtatacag
     1261 atacctagat ggaacacact acttgaaata gccataaaat gtcaaaaagt tacaggtttg
     1321 ggatatgtag gtgtagatat agcacttgat gctgataaag gacctgttgt atttgaagta
     1381 aatgcaagac caggatttgg tattcaggtc gcaaatcaag caggattgag atggagatta
     1441 gaaaaagtta aagatgtaaa aataaaaggt ataaaacatg gaataagagt ggcaaaaagt
     1501 ctctttggag gagaagtaga agaaagtata gaagctataa gtggaagaaa ggttcttaac
     1561 attgttgaaa gaatatatgt atatgataaa aaggctgata gatcaaaact tgaaaaatac
     1621 aaagatagta aaagagagct tgtaaaagca tatatgaaca caggggtgct tacatcaaga
     1681 attgattcaa aacttgccaa tagaattggg tattttaatg accataaaca gtttgaaaaa
     1741 tatggaatac ctaagaaatt tgaaagtctt aaagatgctc aggactttat tgatagaaat
     1801 gagaaagaga taataaagaa tagcagtata aaaagacttg caaaaattgt agaaaatggg
     1861 gaaataaaag taagacctgt atttgatatc cctataaaga tagaagatca gataaaaatt
     1921 acagagttca tatcaacaga aatctctgat atctctcagc cagtaattat tggtaagacc
     1981 gatcttacag gttacctaat agatacaacc aatacatttt aatatatact aaacatatga
     2041 caaaatcata cgaacaatac ttaaaagaag caggaaatgt acatcttgct tactatttag
     2101 aagcagcaga tgagttaaat ataaaatata ctgttttagt acatggcctt cttgcaaagt
     2161 ttgaaacaga aaataaacac tggtacataa taaatactgc tactccactt acaaatacac
     2221 caagtacaac aatagctaaa agaaaaaatc ttacaaataa gatactttct tcttttagta
     2281 tcccagttcc taaacaagtt acactaaata ataaagatga tgcattaagg ttttttaagg
     2341 agtataaaaa tattgtaatt aaaccttcac agcagttagg aggaattggt gtctcactgc
     2401 ttccacaaaa caaaaaacaa gttgttaatg catttaattc agcacttcgt gtatcaaagg
     2461 ggagtcataa agtaattggt gaggagtttg tagaaggtga taattataga cttctagtac
     2521 ttggaaatag agtaattggt attgtaagaa gaaaacctgc ctttgttgtc ggcaatggaa
     2581 aagatagtat acaaacatta ataaaaaaga aaaatgtaga aagaaaaaag aatcttatga
     2641 agccaattcg tattgataat gaggtacacc aaagacttga gaacaagggt ataagtctta
     2701 aatatatccc taaagaaaat gaagaagtaa tacttagata caactgtaat ctcacaacag
     2761 gaggaactac tcaggagtgt gcatcgcaaa cacatcaata ctacaaagat attgctgtaa
     2821 atgcagtcca tgcaatagga gcaaagtttg gaggagttga tataattaca caggacataa
     2881 caaaacctac gaaatgtgct gtaaatgaaa taaattataa tcctgggttg agacttcact
     2941 acaaagttga cgaaggagag gtagtaagag tagcagttcc aatccaagag tacatatcta
     3001 aaatgattta gtacaagcat gatacaaaca atacaaggaa ttaatctgga atctgagaca
     3061 tcaaaagtta aaattccttt aaatggaaat aacattggag atataactga acttcttgat
     3121 acaatttcat ctctgcatac tatcttcata aatgaataca caatctctcc ttctctttta
     3181 acaatatctt caaaacttcc atttctatgg agagaatctg cagaaacatt acaaaaactt
     3241 tcaaaacaag agattacttt aaaccaagca aaagaattaa ttataaatgt tttgattaat
     3301 aaaagaatta aatcaatgtc tactatttcg cttcttgaat cctgtatgaa gaaaaatatt
     3361 gaaataaccc cttcaattat tgaatctgct gaaatgatta cagaaaaaga agaaataggc
     3421 aaaggagcaa atagatactt caccttagga tgtggaagag gatcacagat aacatgctct
     3481 gcttcttcaa caaaagatgc atatatagca cagaaattac aaagggataa atggataact
     3541 aatacttttt tacaaagact tggtatgaca cttcctaaat gggaattact cccaaataaa
     3601 tcatatatta aaaaggtctg ggataaatat gaaaaacctg tagttataaa accaacaggc
     3661 ttaacagctg gcaagggtgt tactgtagga atagatacta ttcaaaaggc gcaagatgct
     3721 tttgattatg caaaagctgc aatagatacc agagtaagac aggcatggca agaaaaaata
     3781 atgattcaag agcaggtata cggagaagat tacaggctcc ttgttataaa tggaaagctt
     3841 caaattgcaa caaagagaat ccctgctttt gtgactggag atggaagcaa aagtattaaa
     3901 gaactaattg agcaggaaaa ttcagatcca agaagagat
//
LOCUS       DAOSAB010000036         3933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_15426_length_3933_cov_2.939144, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000036 DAOSAB010000000
VERSION     DAOSAB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15426_length_3933_cov_2.939144"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PK432_01115"
     CDS             complement(<1..659)
                     /locus_tag="PK432_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOK59560.1"
                     /translation="MKITVIGGGTGSTTVLEGLKKKKDLDLNVIVGMMDDGGSNAVVR
                     DEFGLLPLSDLRKSIIALSEENENQMLRNLFTYRFSQGDGLKGHTLGNLLMIAMTEIT
                     GSEIEAIEMFKYFFSVTGNIIPVTLDKVRLVAEYDDGSKVVGEHLIDEPEDDKKIVKF
                     YFDKKAQAYQGAIDTIMSSDYIIIGPGDLYTTTLPNIIVEGIPQALQETKAKLIFISN
                     L"
     gene            721..1260
                     /locus_tag="PK432_01120"
     CDS             721..1260
                     /locus_tag="PK432_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOK59561.1"
                     /translation="MDFKVGSKVFYPSHGAGWIKEQKEIEFSGQKKMYYEFEFINSPI
                     SISTPLDNVNNLNIREVEPPEKIKEKISVLKKTPSKDPKTKDFNKLIEIFKKLADQAT
                     IESAIETIQYCNFVKKIREKDGRLIPVSIENELNRAVGDIVGELAVSSGTKFETAAKT
                     FEKVTGIKADITQSKYYNR"
     gene            complement(1240..>3933)
                     /gene="ileS"
                     /locus_tag="PK432_01125"
     CDS             complement(1240..>3933)
                     /gene="ileS"
                     /locus_tag="PK432_01125"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOK59562.1"
                     /translation="HVLTMVIKDVVPRYKTMKGFRVERSLGWDCQGIPVEYEVEKQLG
                     FEQKRDIEKFGVEKFNQLCRESVKKYQGAIRELTKRMGRWVNSDEEYATMDSKYIESI
                     WWSLKELYEKGLLYEGYKVVPYSTRAGTTLSNSEVALGGYETIVDPAVVVKLKLIDSN
                     TYLLIWTTTPWTLPGNLLVAVGKDFDYVEVEYKGERYIVGEKLAKNIFKDDYTVLRKF
                     KGSELIGKEYEPLYPYYKEKRNEGAFRVVHAEHVTLDDGTGLVHQAPYGEEDFVLMSN
                     MGIGMFDYLDDQGNMKDEITQFAGMFYKKANKYIMQDLQERGLLFDSYDYEHQMPMCW
                     RTHTPLIYKPIKSWYIKVTEIKELLVSENEKTNWMPKHFKEGRFGNWLADARDWALSR
                     NRYWGTPLPVWICDKCGKKDVIGSFEELKLKSGVELKDPHKPFVDEIAYKCECGGQMR
                     RVNDVIDVWYDSGSMPFARFHYPFENEEKFKEKFPAEFIGEGVDQTRGWFYTLHVLGC
                     ALFGSRAFNNVIVNGMALAPDGTKMSKSKRNYPEVDSVINSMGADALRLYFLSSPIVH
                     GQDVIFNDSYVKEIVSTVILPYYNCVKYLLSYKEQFKWKEDKDFVPTHVMDRWIFAKT
                     NKTIKDVTRYMDKYNLQGAVNSIFDLIDVLSKWYIRRGRERFVNGDTQALNTLYIVLL
                     QISKLLAPFAPFISESGYLSLVTDKKESVHLEDYPAVENKYIDNELLENMNTVRDICS
                     LGLNIRDENRIKLRQPLLRAYVSIKDKEMQEIIKGELNVKEIVFSEKEVSGNNIKSMR
                     SGDNFVSLDINVSEELLKEGILNEILRALQVARKEKGCKVGEFVDIEYTADSKEILSV
                     IQENREKIMKEIHITSMKENGNVQDGKTIKIGEGSLSVVIL"
BASE COUNT         1284 a          841 c          463 g         1345 t
ORIGIN      
        1 ataagattag aaatgaaaat aagttttgct tttgtttctt gtaatgcttg aggaatgcct
       61 tctacaataa tattcggaag tgtagttgta tataaatcac ctggtccaat aattatataa
      121 tctgaagaca ttattgtatc aattgctccc tgataagctt gtgccttttt atcgaagtaa
      181 aactttacga tctttttatc atcctctggc tcatctatta aatgctcacc cacaaccttt
      241 gaaccatcat catattctgc aacaagacgc accttatcaa gagtaactgg aataatattt
      301 cctgttacag agaagaaata tttaaacatc tctattgctt ctatttcaga accagttatt
      361 tctgtcatag ctatcattaa aagattccct aatgtgtggc ctttaagtcc atctccttga
      421 gagaatctgt atgtaaaaag attcctaagc atttgattct cattttcttc tgaaagtgca
      481 attatagatt ttcttaaatc actaagaggt agcaatccaa actcatctct tacaacagca
      541 ttacttcctc catcatccat cattcctaca ataacattaa gatcaaggtc ttttttcttt
      601 tttaaccctt ccagtacagt tgtagaacct gttcctccac ctattacagt aattttcata
      661 gctataattg tataccaaaa aatgatataa ttaaatgttt aattctcata caaagcaatt
      721 atggatttca aggttggttc aaaagtgttt tatccctcac acggtgctgg ttggataaaa
      781 gaacaaaaag agatagagtt tagtggacaa aagaaaatgt attatgaatt tgagtttatt
      841 aactccccta tttctatctc aacaccactg gataatgtta ataatttaaa tataagggag
      901 gtagaacctc ctgaaaaaat aaaggagaag atttctgttc ttaaaaaaac accaagtaaa
      961 gatccaaaaa caaaggattt taacaaatta attgaaattt ttaagaagct tgcagatcaa
     1021 gcaactattg agtctgcaat agaaacaatt cagtattgta attttgtaaa aaagataaga
     1081 gaaaaagatg ggagattaat cccagtatca attgaaaatg aactaaatag agctgttgga
     1141 gatattgttg gtgaactagc tgtatcatca gggacaaagt ttgaaacagc tgctaaaacc
     1201 tttgaaaaag ttactggtat aaaagctgat ataactcaat caaagtatta caacagataa
     1261 gctaccctca cctatcttaa tagtctttcc atcttgaaca ttcccgttct ctttcatgct
     1321 tgttatatgt atctctttca tgattttctc tctattttct tgtataacag aaagaatctc
     1381 tttactatca gctgtatact caatatcgac aaactcgcct actttacatc ctttctcttt
     1441 tcttgcaact tgaagagctc ttaatatttc atttaatata ccctctttta aaagttcttc
     1501 acttacattt atatccaagc ttacaaaatt atctccgctt ctcattgatt ttatattatt
     1561 cccacttacc tctttctcag aaaatacaat ctctttaaca tttagctctc ctttaattat
     1621 ctcctgcatc tctttatcct ttatagaaac atatgctcgc agtaaaggct gtcttaattt
     1681 aattctgttc tcatcacgaa tattcagacc tagagagcat atatctctaa ctgtattcat
     1741 gttctctaaa agttcattat caatgtattt attttctaca gcaggataat cctctaagtg
     1801 aacagattct tttttatcag ttactaaaga aagatatcct gactctgata taaagggagc
     1861 aaatggagca agaagtttag aaatttgaag taaaacaatg tataaagtat ttaaggcttg
     1921 tgtatctcca ttaacaaatc tctctcttcc cctccttata taccattttg aaagaacatc
     1981 tattaaatca aatatgctat ttactgcacc ctgcagatta tatttatcca tatatcttgt
     2041 aacatccttt attgttttgt ttgtcttcgc aaaaatccat ctatccatca catgagtagg
     2101 aacaaaatct ttatcttctt tccacttaaa ctgctcttta taacttaata aatatttaac
     2161 acagttataa tatggcaaaa taacagtaga aactatctct tttacatatg agtcattgaa
     2221 tattacatcc tgcccatgaa caataggaga gcttaaaaag taaagtctta atgcatctgc
     2281 tcccattgaa ttaattacac tatctacttc aggataattt cttttactct ttgacatttt
     2341 tgttccatca ggagcaagtg ccataccatt aacaatcaca ttattaaatg ctctacttcc
     2401 aaacaatgca cagcctaaaa catgaagggt ataaaaccag ccccttgttt gatcaacacc
     2461 ttctccaata aactcagcgg gaaacttttc cttaaacttc tcttcattct caaaaggata
     2521 atgaaacctt gcaaaaggca tagaaccaga gtcataccat acatcaataa catcattaac
     2581 tcttctcatt tgtcctccac actcacattt gtatgcaatt tcatcaacaa aaggtttatg
     2641 aggatctttc aactctactc cacttttaag ttttagctct tcaaatgaac caattacatc
     2701 cttctttcca catttatcac aaatccaaac aggcaaaggt gtcccccaat atctattcct
     2761 gcttaaagcc caatcccttg cgtcagcaag ccaattccca aatctcccct ccttaaaatg
     2821 tttaggcatc cagtttgttt tctcattttc agaaacaagt aactccttaa tttctgtaac
     2881 cttaatgtac caacttttaa taggtttata aatcaaagga gtatgagtcc tccagcacat
     2941 aggcatttga tgttcataat cataagaatc aaaaagcaaa cccctctcct gcaagtcctg
     3001 cataatatat ttgtttgcct ttttataaaa cattcctgca aactgtgtaa tttcatcttt
     3061 catattccct tgatcatcaa gataatcaaa cataccaata cccatattac tcataagtac
     3121 aaagtcttct tcaccatacg gtgcctgatg aacaagtcca gtaccatcat ctaatgttac
     3181 atgctctgca tgaacaactc taaatgcacc ttcattcctt ttctccttgt aatatggata
     3241 taaaggttca tactcttttc caataagctc acttccttta aattttcgaa gcacagtata
     3301 atcatctttg aaaatatttt tagccaattt ctccccaact atatacctct cccctttata
     3361 ctcaacctca acataatcaa aatcttttcc aacagcaact aaaagattcc ctggaagtgt
     3421 ccatggagta gtagtccaaa ttaaaagata tgtattacta tctattaatt tcagcttaac
     3481 aacaacagca gggtctacaa ttgtttcata tccccccagt gcaacttcag agttagatag
     3541 tgttgtacct gctcttgtag agtatggtac aaccttatat ccctcatata ataatccttt
     3601 ttcatacagc tcttttaatg accaccatat actttcaata tattttgaat ccattgttgc
     3661 atactcttca tcactgttta cccacctacc cattctctta gttaattccc taattgcacc
     3721 ctgatatttt ttaacagact ccctgcataa ctgattaaac ttttcaactc caaatttctc
     3781 aatatctctt ttctgctcaa acccaagctg cttttcaacc tcatactcaa caggtatccc
     3841 ctgacaatcc catcccaaac tcctctcaac cctaaagccc ttcatcgttt tataccttgg
     3901 tactacatct ttaataacca tagtaagcac atg
//
LOCUS       DAOSAB010000037         3928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_15445_length_3928_cov_2.422153, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000037 DAOSAB010000000
VERSION     DAOSAB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15445_length_3928_cov_2.422153"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3778
                     /locus_tag="PK432_01130"
     CDS             <1..3778
                     /locus_tag="PK432_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59563.1"
                     /translation="GPNATLNNIGGTAIRIMGVKFENIKAPRDNNGKLIPGITGYRIL
                     RGSRNGNKTIIAKGIINNLHEYEIPESGGKKSLYPNYPYNDLRNDPFLLNSPSKTTPC
                     ILAGSGINNATTNNPLTPFSYTPGQGLDYKQGSSFKRDYFTFHSPDTNFNNPFLAAKE
                     FRIYGNAVGKVEGKFELSEKHPQEKLISDFSFVIAAIGGIGIAVLNQNGERRINKKFP
                     HYQGFSHRETYSPSTNYSLAHGGTNTATHLPGQTTTTTPLPPATIATAASLVNLVTIP
                     EPPVVTTVTETTTGSTTNIAPISVNPASGPDPFFLGAITGLPATLAASLFANYTTPMN
                     TLAPLTQATTGVLVTDAAATTTQTIQNGQVGVAAGAYGYSTTEIEQTDGALHRTPPLF
                     GGPFFSIPTFFNYLTDGADSVIRLIRAIVKYRDFAVRYHSHGFYDTLNLRPVNMTFRT
                     ELKNQQYINPEILQYDTTYKINNLYRQRTVAINTTTTIIDPIIDDRTRYTNSQLTPPS
                     TKIQDLCNRRITNSITSTVDGLSGQICSSWYGALKVRIRNQYGQLNNIVSLPTSPCYI
                     DVAFDPVTGNILPNKGDSTPVLFGGDVYVNRYTEKNTFLFYYDWLYGQPDGAQFNYKQ
                     HEMIPYPKYWADFSQFQTNDFTSSFLNNILTPTWPPGGNLVTPSDYYVLDGPQCGSFW
                     TGLMTTFQFDKKGWFYLFNSGVRDFFVESEINVAYRDYGEPQDQRFYDPYSGSDTKDL
                     FDTKIIKSGNYYKYDKSLSISQLFLNYTSWASMQATTYDPYIAETCFVYQPKKLIYSL
                     PAQFEGLKDGWLAFLPNNYYNFQNLVTCIKPVNKSGAMIFFDAASPVQFQGLDQLQTD
                     LGTKLTIGDGGLFSQPVQALINVDTSHEYASCQNRLSVINTPAGLFWISQNQGKVFSL
                     TGSIKELSNESLKWWFAEYLPYKIVEEFPDFDLLDNPVIGVGCQSVYDNQNGLIYFSK
                     IDYSLRKDISETLTYIKGSNKFTVDQTGLTVELGDPLFFNDASWTTSYDPKTGAWIGW
                     HDWHPTLNMPGKNTFMTINPNDKRGIWIHNVRCDLYCNYYGIDYPFEVEFMVNTGQTV
                     NTLRSIQYIMEAYKYAKNCYDRFHVLDYNFDEAIIHNTEQVSGLLKLNLNPKDNPTAI
                     LGYPVIGVTDIQILFSKEENKYRFNQFWDVTDDRGEFFNPAIPGFAQRPIWNTEDNGY
                     IRLLNPINLNYAKNPFQRKKFRHYTTSVWLCKKVSGDKKMLVMIADTKELYSPR"
BASE COUNT         1300 a          661 c          691 g         1276 t
ORIGIN      
        1 tggacctaat gctactttaa ataatatagg tggtacagca ataagaatca tgggtgttaa
       61 gtttgagaat atcaaggcgc ctagagataa taatggaaaa cttattcccg gcattaccgg
      121 ttatagaatc ttaagaggat ctagaaatgg taataaaaca attattgcta aaggaattat
      181 taataatctt catgaatatg agattcctga atcaggaggt aagaaatcat tgtaccctaa
      241 ctatccttat aatgatttaa ggaatgatcc ttttttatta aattctccta gtaagactac
      301 tccttgtatt cttgctggca gcggtatcaa taatgctact acaaataacc ctcttactcc
      361 ttttagttat actccaggac aaggtttaga ttacaaacaa ggaagctctt ttaaaagaga
      421 ttattttact tttcattctc ctgatactaa ctttaataat cctttcttag cagctaaaga
      481 attcagaata tatggtaatg ctgttggtaa agttgaaggt aaatttgagt tatcggaaaa
      541 gcatcctcag gaaaaattaa tttcagattt ttcttttgtt attgctgcta ttggcggtat
      601 aggtatagcg gttcttaatc aaaatggtga gagaagaatt aataaaaaat ttcctcatta
      661 tcaaggtttt tctcatagag aaacttattc tcctagtaca aattattctt tagctcatgg
      721 tggtacaaat acagcaactc atttgcctgg gcaaacaaca accactacac cattgccgcc
      781 ggctacaata gcaactgcag cttctttggt taatttggtt acaataccag aaccaccagt
      841 tgttactaca gtaactgaaa ccactacggg aagtactaca aacatagcac caatatccgt
      901 aaatcctgca tccgggcctg atcctttctt tttaggtgct ataacaggat taccagctac
      961 attagccgct tctttatttg ctaattatac tacaccaatg aacactttag cgcctttaac
     1021 tcaagctact accggtgtat tggtaacaga tgctgcagca acaaccactc agactataca
     1081 gaatggtcaa gtaggtgttg cagccggagc ttatggttat tcaactacag aaattgaaca
     1141 aacagatggc gctttacata gaactcctcc tctttttgga ggaccttttt tctccattcc
     1201 tacattcttt aattatttaa cagatggagc tgattcagtt ataagattaa taagagctat
     1261 tgttaagtac agagactttg cagtaagata tcattctcat ggtttttatg atactttgaa
     1321 tcttagacca gttaatatga catttaggac agaacttaaa aatcaacagt atattaatcc
     1381 tgagatatta cagtatgata caacctataa aatcaataat ctttacagac aaagaactgt
     1441 agctattaat acaactacta ctattataga ccctattata gatgatagaa cccgatacac
     1501 aaatagtcaa ttaacacctc cttcaactaa aattcaagac ttatgtaata gaagaattac
     1561 aaatagtata acctcaaccg tagatgggct ttcgggtcaa atctgttctt catggtacgg
     1621 tgctcttaaa gtaagaatta gaaatcagta tggtcagtta aataatattg tttctttacc
     1681 tactagtcct tgttacattg atgtagcctt tgatcctgtg accggaaata tacttccaaa
     1741 taaaggtgat tctacgccgg tgttatttgg tggagatgtt tatgtaaaca gatatactga
     1801 aaaaaatact ttcttattct attatgattg gttatatggt caacctgatg gtgcacagtt
     1861 taattacaag caacatgaga tgataccgta tcctaagtat tgggcagatt tcagtcagtt
     1921 tcaaactaat gattttacat ctagtttttt aaataacata ttaaccccta cctggcctcc
     1981 gggaggaaac ttagttacac ctagtgatta ctatgtttta gatggaccac aatgtggaag
     2041 tttttggacc gggttaatga ctacttttca gtttgataag aaaggttggt tctatttatt
     2101 taactcaggt gtgcgagact tctttgtaga aagtgagatc aatgttgctt accgtgatta
     2161 tggcgagcct caagaccaaa gattctatga tccttattct ggcagtgata ctaaagatct
     2221 gtttgataca aaaataatta aatcgggtaa ctactacaaa tatgataaat cattaagtat
     2281 ctctcagttg tttcttaact atacatcctg ggcaagtatg caggctacaa cctatgatcc
     2341 atacattgct gagacatgtt ttgtatatca acctaagaaa ttaatctatt ctttacctgc
     2401 acagtttgaa ggattaaaag atggttggct agctttctta cctaataact attataattt
     2461 tcagaactta gttacatgca ttaagccggt taataaaagc ggagctatga tcttctttga
     2521 tgcagccagt cctgtacagt ttcaaggttt ggatcaactt caaacagatc ttggtaccaa
     2581 acttactatt ggtgatggtg gactatttag tcaacctgtg caagctttaa ttaatgtaga
     2641 tacatctcat gaatatgctt cctgtcaaaa ccggttaagt gttattaaca ctcctgctgg
     2701 tttattctgg ataagtcaga atcaaggtaa agtgtttagc ttaaccggta gtataaaaga
     2761 attgtctaat gaaagtttaa aatggtggtt tgccgagtat cttccttata agatagtaga
     2821 agaattccct gattttgact tattggataa tccggttatt ggagtaggat gtcaatctgt
     2881 ttatgataac caaaatggat taatctattt ctctaagatt gattacagtt taagaaaaga
     2941 tattagtgaa acacttactt atattaaagg ctcaaataaa tttacagtag atcaaacagg
     3001 attaactgtt gagttaggag atcctttgtt ctttaatgat gcttcttgga ccacaagtta
     3061 tgatcctaag actggtgcat ggattggctg gcatgactgg catccaacat taaatatgcc
     3121 gggtaagaat actttcatga ccattaatcc taatgataag agaggtatct ggatacacaa
     3181 tgtgagatgt gatctatact gtaactacta cggtattgat tatccatttg aagtagagtt
     3241 catggtgaac actggtcaga ctgttaatac cttaagaagt atccagtata ttatggaagc
     3301 atataagtat gctaaaaatt gctatgatag attccatgtt ttagattaca actttgatga
     3361 agcaattatt cataacacag aacaggtctc tggtttatta aaactgaacc ttaatccaaa
     3421 agataatcct acagcaatct taggatatcc tgttattggt gtaactgata ttcagatctt
     3481 attctctaaa gaagagaaca agtatcgctt caatcagttc tgggatgtta cagatgatag
     3541 aggagaattc tttaatccgg ctattccagg atttgcacaa aggcctattt ggaatacaga
     3601 agataatggt tatattagat tattaaatcc aatcaatctt aactatgcta aaaatccttt
     3661 tcagagaaaa aagttcagac attatacaac ctctgtttgg ttatgtaaga aggtctctgg
     3721 agataaaaag atgttagtaa tgattgctga tacaaaagaa ctttattcac caagataatg
     3781 agcaacggta aaacaataag agaagctatg aaggctgcag gagatactcc taagaaacct
     3841 aatccataca agcatgatgt tatttatgat ccccgagggc aatgggcctt ccctgggcaa
     3901 gttactaaaa ttccttctag taatatta
//
LOCUS       DAOSAB010000038         3903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_15562_length_3903_cov_3.298857, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000038 DAOSAB010000000
VERSION     DAOSAB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15562_length_3903_cov_3.298857"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1014
                     /locus_tag="PK432_01135"
     CDS             85..1014
                     /locus_tag="PK432_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOK59564.1"
                     /translation="MGYSQVVKEYVKVRAEQTENNTVIAREVKAKFNPPQELDKIRRW
                     ISCFRKRLKIKAKQQPIKRLFFDIETSYYTCRLWHIGKVDYVSPETIITPKQIICIAY
                     KWQHEDEVHILDWTMGEKEMLKAFVEVLAEASEIIGHNCNKFDLKELRTRCIYYGLPM
                     FPTYRTLDTLLKSREYFNFASNKLDYIGKYLQVGKKLDHTGFQLWIDVVEKRDPDALK
                     LMLDYCIQDVVLLEDVYTVLSPYITHNNNFAVLTGGEKWECPECTSNDVVLSHTYATA
                     MGTIKRNMKCNACGKQYKISNKSYIKMLEKSMK"
     gene            1074..1328
                     /locus_tag="PK432_01140"
     CDS             1074..1328
                     /locus_tag="PK432_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59565.1"
                     /translation="MESKEIKKEGELSTDPMSQKAYKEHMKNAINLLKLEHEYEKLRS
                     EILQYKLISYRCIEGLAAYQGKEQSTDKQATSTVEKSPTK"
     gene            1423..1815
                     /locus_tag="PK432_01145"
     CDS             1423..1815
                     /locus_tag="PK432_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59566.1"
                     /translation="MGIEVKKVEKKYRLSRYEAVRYQLLTELIFLRKEQVTLSDIDLL
                     ALLAIYKVIELSKFCTIATNYFHPDIPNHQFVNKSQNIRNKLNKLLKQGIINRDNQRI
                     SLNNIPVVTDKAMLLTYNFLSVDGSDKA"
     gene            1796..2185
                     /locus_tag="PK432_01150"
     CDS             1796..2185
                     /locus_tag="PK432_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59567.1"
                     /translation="MAATKRKYLVEKTAKELNMSADDIDDIVYSYYNHVLKLINSLEH
                     TRIYVNNVCTFKFRVAKSAAYIQHAEKLLERWRLRKPTKYLLESISEVENRVIKLKKL
                     IEIMYQQYEVKKIKRKERYEFIKSMAS"
     gene            2157..2447
                     /locus_tag="PK432_01155"
     CDS             2157..2447
                     /locus_tag="PK432_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59568.1"
                     /translation="MSLLKVWQARNQILEGILNRIFTKEHVEEIALERMSICKDCEHY
                     GTECLMPGTAPCCAICGCCLELKTRSLSSNCPEHKWEAIMTEEEEDLLNEQL"
     gene            2459..3073
                     /locus_tag="PK432_01160"
     CDS             2459..3073
                     /locus_tag="PK432_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59569.1"
                     /translation="MIKFHILDKTRKDQYYLPSVIQALTEQGWLQYKHHPVFYKKNEN
                     LINGDYTIMIPKVEILKCELITNNNYQRQLIVDFNNIINNKILHEHSGWHVKYIRLED
                     WNTLEEPLLELTIRMYKEGGCYTEALKYCPKGQEKYYNLAWAKYYKLTDYNRKKINII
                     TILIKPYYYFYNILNSVKIFILLNWYKLSLWFYKHDTIGQILNK"
     gene            3091..3891
                     /locus_tag="PK432_01165"
     CDS             3091..3891
                     /locus_tag="PK432_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59570.1"
                     /translation="MTLKFKPDNHQYISDDGTRWTSVTTIVSAYKQPFDAQTIAEKCS
                     KNKKSKWYGIDPKEIQAIWKAEADRSTTLGTYYHKQREKDLLNCDTLTRYGYELPIIP
                     SLEDDNGCKIAPEQKLTDGIYPEHFIYLASAGWCGQADLVEVVNGVVNVSDYKTSKKI
                     DRESYKNWEGISKKMLSPLQHLDDCHFNHYALQLSIYMYMILRHNPNLKPGKLLIHHV
                     KFEEAEEKDKYGFPIILLNNGEPIVKEIEYIEVPYLKQEVQTLLKQKI"
BASE COUNT         1650 a          413 c          615 g         1225 t
ORIGIN      
        1 aaacaatttc tttcttatta aacttgtata gataatgata ttatattatt atttgtgcca
       61 ttagtgtaat taaaaatcag aaatatgggt tattcacaag tagtaaaaga gtatgttaag
      121 gtcagggctg aacagactga aaataatact gtgattgcta gagaagtaaa agcaaaattt
      181 aatccacctc aagaactaga taaaatcaga aggtggattt cttgttttag aaaaaggctt
      241 aaaattaaag ctaaacaaca gcccataaaa aggttatttt ttgacataga aacaagttat
      301 tatacttgta ggttatggca tataggaaag gtggattatg tctctcctga aacaattatt
      361 actccaaaac agataatatg tatagcttat aaatggcagc atgaagatga agtacatata
      421 ttagattgga caatgggtga aaaagaaatg ttaaaagcat ttgtagaagt actagcagaa
      481 gcttcagaaa ttattggaca taattgtaat aaatttgatt taaaagaact tagaactaga
      541 tgtatatatt atggattacc aatgtttcct acatatagaa ctttagatac acttttaaaa
      601 tcaagagaat attttaactt tgcttctaat aaattagatt atattggtaa atatttacaa
      661 gtaggtaaaa agctagatca tacaggattt caattatgga ttgatgttgt agagaaaaga
      721 gatcctgatg ctttaaaatt aatgttagat tattgtattc aagatgtagt actattagaa
      781 gatgtatata ctgtattaag cccttatata acccacaata ataactttgc tgtattaaca
      841 ggtggagaaa agtgggaatg cccagaatgt acatctaatg atgttgtgtt gagtcatact
      901 tatgctacag ctatgggtac tataaaaagg aatatgaaat gtaatgcttg tggtaaacaa
      961 tataagatta gtaataagag ttacataaaa atgttagaaa aaagtatgaa ataaatttgt
     1021 atatttaaaa taaatgtttt atatttgcat tataaacttt aaaatttaaa aaaatggaat
     1081 caaaagaaat taaaaaagaa ggagaattat ctacagaccc aatgtctcaa aaagcttata
     1141 aagagcatat gaagaatgcc attaatttac taaagcttga acatgagtat gaaaaactta
     1201 gatctgaaat tttacagtat aagcttatca gttatcgatg tatagaagga ttagcagcat
     1261 atcaaggtaa agaacaatct acagataaac aagctacttc tacagtagaa aaaagtccta
     1321 caaagtaata aacaattgtc tgatagtgta atagtagcac ctctgttttt gaaacagata
     1381 gtctagattc aagtcctagt cagacatcaa ataaagaaat caatgggaat agaagttaaa
     1441 aaagtagaaa agaaatatag gttatctcgg tatgaagctg taaggtatca attacttaca
     1501 gaacttatat tcttgcgtaa agaacaagtt acattaagtg atatagattt attagcttta
     1561 ttggctattt ataaagtaat agagcttagt aagttttgta ctattgctac taattatttt
     1621 catcctgata tacctaatca tcaatttgta aataaatctc aaaatataag aaataaatta
     1681 aataaactat taaagcaagg tattattaat agagataatc aaagaatatc tttaaataat
     1741 ataccagttg ttacagataa agcaatgtta ttaacttata attttttaag tgtagatggc
     1801 agcgacaaag cgtaaatatc ttgtagagaa aactgctaaa gaattaaata tgtcagcaga
     1861 tgatatagat gatattgttt attcatatta taatcatgta ctgaagttaa ttaattcatt
     1921 agagcataca cgaatttatg tgaacaatgt ttgtacattt aaatttagag tagcaaaatc
     1981 agcagcatat atacaacatg ctgagaaatt attggaaaga tggagattaa gaaaacctac
     2041 aaaatattta ttagaaagta tatcagaagt agaaaacaga gtaattaaac ttaaaaaatt
     2101 aattgaaatt atgtatcagc aatatgaagt gaaaaaaatt aaaagaaagg aaagatatga
     2161 gtttattaaa agtatggcaa gctaggaatc agatactgga agggatatta aatagaatat
     2221 tcactaaaga gcatgttgaa gaaattgctt tagaaagaat gagtatttgt aaagattgtg
     2281 agcattatgg tacagaatgt ttaatgccag gcacagcacc ttgttgtgca atatgtggat
     2341 gttgtttaga attaaaaaca agatctttat cttcaaattg tcctgaacac aaatgggaag
     2401 caataatgac agaagaagaa gaagatttat taaatgaaca attataaaag tatttaatat
     2461 gataaaattt catattttag ataaaacaag aaaagatcaa tattatttac catctgttat
     2521 tcaagcattg acagaacaag gatggttgca atataaacat catcctgtat tttataaaaa
     2581 aaatgaaaat ttaattaatg gtgattatac cataatgatt ccaaaagtag aaatattaaa
     2641 atgtgaatta ataactaata ataattatca aagacaatta attgttgatt ttaataatat
     2701 tattaataat aaaattttgc atgagcattc aggatggcat gtaaaatata ttagattaga
     2761 agactggaat actttagaag aacctttatt agaattaacc ataagaatgt acaaagaagg
     2821 aggatgttat acagaagcat tgaaatactg tcctaaagga caagagaaat attataacct
     2881 tgcatgggct aaatattata aattaactga ttacaataga aaaaaaatta atataattac
     2941 tatattaatc aagccttact attattttta taatatatta aactcagtaa aaatttttat
     3001 tttactcaat tggtataaat tatcactatg gttttataaa cacgacacaa taggacaaat
     3061 tcttaataaa tagttaaata aaaaataaaa atgactttaa aatttaaacc agataatcac
     3121 caatatatat cagatgatgg tactagatgg actagtgtaa cgaccattgt atctgcttat
     3181 aaacaaccat ttgatgctca aaccatagct gaaaagtgtt ctaaaaataa gaagagtaaa
     3241 tggtatggta tagatcctaa agaaatacaa gcaatttgga aagcagaagc agatagatca
     3301 actactcttg ggacttatta tcataagcaa agagaaaaag atttattaaa ttgtgataca
     3361 ttaactaggt atggttatga attacctata ataccttctt tagaagatga taatggttgt
     3421 aaaatagcac cagaacaaaa gttaactgat ggtatatatc ctgaacattt tatttattta
     3481 gcatcagcag gatggtgtgg acaagcagat ttagtagaag ttgtcaatgg tgtagttaat
     3541 gtatctgatt ataaaacatc aaaaaagatt gatagagaat cttataaaaa ttgggaaggt
     3601 atttctaaga aaatgttatc acctttacaa catttggatg attgccactt caaccattat
     3661 gctttacaat tatcaatata tatgtacatg atattaagac ataatcctaa tttaaaacca
     3721 ggtaaactat taatacatca tgttaaattt gaagaagcag aagaaaagga taaatatgga
     3781 tttccaatta tactattaaa caatggtgaa ccaattgtaa aagaaataga atatatagaa
     3841 gtaccttatt taaaacaaga agtacaaaca ttattaaaac aaaagatatg ataatgaaag
     3901 att
//
LOCUS       DAOSAB010000039         3902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_15567_length_3902_cov_2.185079, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000039 DAOSAB010000000
VERSION     DAOSAB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15567_length_3902_cov_2.185079"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PK432_01170"
     CDS             <1..1213
                     /locus_tag="PK432_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOK59571.1"
                     /translation="MIAVMLMTFILFGMTDLQDAVLTQFNSVFKPTDLYVSNSSFAMG
                     GVSSAPRKEDVQNEEKVVIDNDVVESIKSIEGVEEIHRIFAVAGFDVYLQGDDIPYPR
                     SLIQAVDLSADNELFVNFTGDDPVLKENGIFVSNYVTSFFELSADEIIGKNIVLKSSQ
                     INNFGSTPNTNTPAQEYTFIVQGVVETDNSAFWINSDRALEILAQRGGFKDKEEYIQT
                     VGYSQLLVSTQDGKTSQVEDALVNDLGLTVISTKTIVDFVSTLTAGLTIALIIFGSIS
                     AIVASIGIINTMIMSIYEQTKEIGIIKAIGASDFQVLVIFLIQAGLIGLIGGLLGLSI
                     TYLLMKVADPYVVKLLSDQGFTAVTTFFHFQPLNALYITLSSILVGIIAGIYPAMKAA
                     KLDPVNALRYE"
     gene            1913..2932
                     /locus_tag="PK432_01175"
     CDS             1913..2932
                     /locus_tag="PK432_01175"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOK59572.1"
                     /translation="MNNEQRDIAEKIKTYIEKAENILLHCHPFPDPDSIGSVLAMKEY
                     LESEGKAVTAIIGDSKYPENLSNLQIEKKILPKNFFEIDSKKYDLFLILDSSSKTQIS
                     RIGEVNFPKGMNTVVIDHHRTNEKFGDVNLIISNYASTTQILYELFKQWEVNISKDMA
                     LYLFLGLFADTGGFKYLNSTPEVLQMGAELSLINPNYHKLVFDLENSRKPIEIEMMAL
                     ALSSIQKYINGKVVFTVIPFEELVKRELDESSAIEGLVPEILRSVLGWEIVASLVEVE
                     KGKTIVSLRTRDEEKYDMSKIASIVGKNGGGHPGAAGTTIMKNAQTTVKELLQAIKKL
                     YGDKL"
     gene            complement(3104..3517)
                     /locus_tag="PK432_01180"
     CDS             complement(3104..3517)
                     /locus_tag="PK432_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOK59573.1"
                     /translation="MKKVYLDTNILIDILLDRDLEHISINKITPFLKQSQVYMSTLSV
                     HITYYILKIKPNTSMHKKSMALINRINLVPLSQEIINQSLNNFSIDFEDTLQYYSALD
                     QNCDYILTRDKKNFKKIKELIPSKIEIIDTLNTIL"
     gene            complement(3514..3810)
                     /locus_tag="PK432_01185"
     CDS             complement(3514..3810)
                     /locus_tag="PK432_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59574.1"
                     /translation="MNIEYKKINLRDFRQNLTQLKDSLNAGQAYEVIDRGKTLAYFIP
                     SEYEVKVNKKTKSTEEIERILTEAIGCVHMSEKKKKTFDYDKEYRKAMEKKHLR"
BASE COUNT         1313 a          521 c          680 g         1388 t
ORIGIN      
        1 tatgatagcg gttatgctta tgacctttat actttttggt atgacagatt tgcaggatgc
       61 tgttcttact cagtttaatt ctgtctttaa acctacagat ctttatgtat caaacagcag
      121 ctttgcaatg gggggtgtat cttctgctcc tagaaaggaa gatgttcaaa atgaagaaaa
      181 ggttgttata gataatgatg ttgtagagag tattaaaagt atagaaggag tagaggagat
      241 acatagaatt tttgctgtag caggttttga tgtatatctg cagggggatg atatccctta
      301 tcctagatct cttattcaag ctgttgattt gtctgcggat aatgaattgt ttgtaaattt
      361 tactggtgat gatcctgtac tgaaagaaaa tggtattttt gtaagtaatt atgttacttc
      421 attttttgaa ctttctgctg atgaaattat tggaaaaaat atagttttaa agtcatctca
      481 aataaataac tttggctcaa cacctaatac caacactcct gcacaagaat atacatttat
      541 agtccagggg gttgtagaaa ctgataacag tgcattttgg attaatagtg atagagcatt
      601 ggaaatacta gcacaaaggg gtggttttaa ggataaagag gaatatattc aaactgttgg
      661 atactctcag ttgcttgttt ctacacaaga tggaaaaact tcacaggtag aagatgcatt
      721 ggttaatgat ttagggctta cagttatatc tactaaaact attgttgatt ttgtttctac
      781 attaacagca ggtttaacta ttgctcttat aatcttcggc tctatatctg caatagttgc
      841 ttctattgga attattaata ctatgattat gagtatttat gaacagacaa aagagatagg
      901 tattattaag gcaataggtg catctgattt tcaagtgcta gttatctttt taatacaagc
      961 aggattgata ggattaatag gtggtctttt gggtttgtct attacatatt tgttaatgaa
     1021 ggttgcagat ccatatgttg taaaacttct ttctgatcaa ggttttactg ctgtaacaac
     1081 atttttccat ttccagccgt taaatgcttt atatattaca ttgagtagta tccttgttgg
     1141 tattattgca ggtatatatc ctgctatgaa ggctgcaaag cttgatcctg taaatgcact
     1201 gaggtatgaa taactttgtt tttaaaaaca gagagacaat attatgatat ttctcttttc
     1261 ctttcttgtt tgttgatttt attagaattc gcttaccctc ttaaagcatt tcatattttt
     1321 taaagatatc aattggaata atatagtcac aagaataata tacaaactat attccttaag
     1381 aaaatctaat aggaaatgtg gtaatacaga agagaacaca cccattagga tacttgcaga
     1441 acagaaataa ccagaagtcc cacatacttg gagtgccatt atccctaaac cgctagctat
     1501 tagattaata acggagtgag tagtcgttct agctaccttt attctttcct taatatttct
     1561 aatcatgcaa gtcatattaa aggagaagac taatataaag acaatgacta ttagattcca
     1621 ccaaaacccg actaaatgtt taggaactaa gaagtaactt aatattagag atagtaaaaa
     1681 tacaatctta cagtatttac tctttaaaat ttctatcaat acaaaatcaa gttttacttt
     1741 ctctgggtgt ttcatcgcat ctatataaat aaatatcttg caatcattat atcaaataat
     1801 tcctggttag aaagttgcta cttgtgatgt ctataaataa tatattctta caaacctcat
     1861 gctgttttta gtataatttt tcagaatata agtataaaaa gtgtgagatg atatgaataa
     1921 tgaacaaaga gatatagcag agaaaataaa gacatacata gaaaaagcag aaaacattct
     1981 tttacattgt catcctttcc cagatcctga cagtattggg tcagtcttag ctatgaaaga
     2041 atatctcgag agcgagggga aagcagttac tgcaattatt ggggattcta agtacccaga
     2101 gaatcttagc aatcttcaaa ttgaaaagaa gatacttcct aaaaactttt ttgagataga
     2161 tagtaagaag tatgatctct ttttaatctt agatagttct tctaaaactc agatctctag
     2221 aataggagaa gtgaattttc caaaaggaat gaatacagta gttattgatc atcatagaac
     2281 taatgaaaaa tttggtgatg taaatctaat cataagtaat tatgcatcaa caacacaaat
     2341 tctctatgag ttatttaaac aatgggaagt gaatatatca aaagatatgg cattgtatct
     2401 ctttctaggt ttgtttgcag atacaggagg gtttaagtat ttaaatagta caccagaagt
     2461 attacaaatg ggtgcagagt tatctttaat caatcccaat tatcataagc tagtttttga
     2521 cttagagaat agtaggaaac caatagaaat agaaatgatg gcattggcat tatcatctat
     2581 tcagaaatat attaatggga aggtggtttt tacagtaatc ccctttgaag aacttgtaaa
     2641 gagagaatta gatgaatcgt ctgcaataga aggattagtt cctgagatat tacgttctgt
     2701 attgggatgg gaaattgtag ctagcttggt ggaagtagaa aaggggaaaa ccattgtaag
     2761 cttaagaaca agagatgaag aaaagtatga tatgagtaaa atagcttcta ttgtaggtaa
     2821 aaatggagga ggtcatccag gtgctgctgg aaccactatt atgaagaatg ctcaaactac
     2881 ggtaaaagag ttactacaag cgattaagaa attgtatggt gataagttat aaagatgtgt
     2941 gatctcataa gtatttatgg ctaataagac atctcacttc ttgaatatgg tattgttatc
     3001 tcttctatca aatcttccca cttttattat ctacttaatt taaaatctcg tacactatat
     3061 atcatttgtt aagatttaac aaggaaaatg gatattctta ttactatagt attgtgttaa
     3121 gagtatctat tatttctatt ttagagggta ttaactcctt tattttctta aaattctttt
     3181 tatctctagt tagaatgtaa tcacaatttt gatctagagc agagtagtac tgtagagtgt
     3241 cttcaaaatc aattgagaag ttatttaagg actggttaat aatttcttgt gaaaggggga
     3301 ctagattgat tctattgatt aatgccatag attttttatg catagatgta ttaggcttaa
     3361 ttttaagaat gtaatatgta atatgtaccg aaagagtaga catatatact tgagattgtt
     3421 taagaaaagg agttattttg ttaatagaga tatgttctaa gtctctatct aaaaggatat
     3481 ctattaatat gtttgtatct aagtatacct tcttcatctt agatgtttct tttccatagc
     3541 ttttctatac tctttatcat agtcaaaagt cttttttttc ttttcgctca tatgaacaca
     3601 tccgattgct tcagtaagta ttctttctat ctcctctgta ctttttgttt tcttattaac
     3661 tttcacttca tattcagaag gtatgaaata tgctaatgtt ttacccctat ctattacctc
     3721 atatgcttgt ccagcattaa gagaatcttt tagttgggta agattttgtc taaaatccct
     3781 taagtttatt ttcttgtatt caatgttcat atgttaaaca ttagtttaac attaagaagt
     3841 gggtaagtca aatatgaaat attaacttga gatatagtat aatgatgcac tatggcaaca
     3901 ta
//
LOCUS       DAOSAB010000040         3846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_15853_length_3846_cov_3.841203, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000040 DAOSAB010000000
VERSION     DAOSAB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15853_length_3846_cov_3.841203"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..229
                     /locus_tag="PK432_01190"
     tRNA            155..229
                     /locus_tag="PK432_01190"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Met,seq:cat)
     gene            complement(233..787)
                     /gene="hpt"
                     /locus_tag="PK432_01195"
     CDS             complement(233..787)
                     /gene="hpt"
                     /locus_tag="PK432_01195"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOK59575.1"
                     /translation="MTEKTEQQDPVFEILFTQEEIEERVLGLAQEISKDYELDNKTPL
                     LLIGVLKGAVPFLTDLSQQINHPNLYIDYMGVSSYNGNTTSNKEPKITLDTKIPIKDK
                     NVIIVEDIVDTGYSIATLINILQARKPKSLKVCSLLSKPERREIKVPIDYLGFTIPNL
                     WVQGYGLDTNEEGRYWPYIACRKE"
     gene            895..1455
                     /locus_tag="PK432_01200"
     CDS             895..1455
                     /locus_tag="PK432_01200"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOK59576.1"
                     /translation="MPLTDQPVKGMYIKEDGRFFFLEDRKLKTQGRQGGLVIMKLKAL
                     DNGQIINKTVKSGTKVEYINPETKEAQYLYKDDSFAYFMDTSTYETVNISKDVIGSYV
                     NFLKEGDSILMLTYEGKILSIKESPSVVLEVTVAQDAVKGNTANNATKLVTVETGYQL
                     KVPLFIKKGDKIKINTETGEYSGKAN"
     gene            1498..1574
                     /locus_tag="PK432_01205"
     tRNA            1498..1574
                     /locus_tag="PK432_01205"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1532..1534,aa:Met,seq:cat)
     gene            1597..3471
                     /gene="ftsH"
                     /locus_tag="PK432_01210"
     CDS             1597..3471
                     /gene="ftsH"
                     /locus_tag="PK432_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOK59577.1"
                     /translation="MEENKKENKQPNVKIIRVTPKKRMSPVMSFVMFLIMSFLLTIGI
                     NAILGSTSKGQEVPLSEVVSYISEGEYKDIVLRDDMVIVTQSVKSDGKDVITKKYALI
                     PVGTDFYGMLSDAGVDIKALKNDYYQPQLGISIGDIISFILLAAGLVLAYMLIKNMQK
                     SGGQIMDFGQSRARLLFGKKTGVTFDDVAGIDEVKEELTEIVDFLKSPKKYIDIGARI
                     PKGVLLVGAPGTGKTLLAKAVAGEAGVPFFHTSGSEFEEMLVGAGASRVRDLFNKARK
                     AAPCIIFIDEIDAVAKKRGTVLHSGAGEQTLNQILVEMDGLEGRENVIVLAATNRPDV
                     LDPAILRPGRFDRTVVVPMPDYQGRKEIIMVHAKGKKFEDSVDLDLIARKTIGYSGAD
                     LENLLNEAAIMAAREGRKKINQNDLTEAYLKVKLGRKRKGQSSEDDLKVTAYHEAGHA
                     IVAKFTEHATPVEQVSIIPRGISGGVTVYLPEDDKKHVFKDELLANITSAVGGRASEE
                     LFIGRISTGASADIEEATSVAKEMVTQYGMSDKLGLVKYGDVEETKHLGYVYGGGRDF
                     SEKYAEMIDSEVKNIMDSAYHQAKKILSDKKEYVEKLVALLIKNEVVSKEEFDALFNE
                     "
     gene            3507..>3846
                     /locus_tag="PK432_01215"
     CDS             3507..>3846
                     /locus_tag="PK432_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOK59578.1"
                     /translation="MIKESEKDTFLAIDANAIVHRAFHAYPSTLQTPDGVQVNAVYGF
                     TVMLLEAFKMFEPKYVLCAMDTHTPTFRHEIYVDYKATRKPTDLSLIDQFPLVEEILK
                     AFNIPVIKKEG"
BASE COUNT         1263 a          520 c          822 g         1241 t
ORIGIN      
        1 agagatggca atggaggaag tagaaaagat aatagcttct tcgcaagagt ctatttatga
       61 aagtggaagt aattttgaag aatggctatc ttttgtttta gagggagttt gaagtaatgt
      121 tgcttttaga gtataatctc acttagatta atatcgcggg gtagagcagt ggcagctcgt
      181 caggctcata acctgaagga cgttggttcg aatccaaccc ccgcaaccat ttttattctt
      241 ttctgcaagc gatatacggc cagtatcttc cttcttcatt tgtatcaaga ccataccctt
      301 gaacccataa attggggatt gtaaatccta aatagtcaat tggtactttt atttcccttc
      361 tctctggttt tgataggaga gaacatactt ttaatgattt aggttttctt gcctgaagaa
      421 tatttattag tgtagcaata gagtatcctg tatctacaat atcttctaca attattacat
      481 ttttatcctt tattggtatt tttgtatcaa gtgtaatttt aggttctttg tttgaggttg
      541 tatttccgtt atagcttgat actcccatgt aatcaatata caggtttgga tgattaattt
      601 gctgtgaaag gtctgttaaa aatggtacag ctcccttaag aacccctatt agcagtaacg
      661 gagttttatt atctaattca tagtctttag agatttcttg tgcaagtcct aagacccttt
      721 cttcaatttc ttcttgggta aacagtatct caaaaactgg atcttgttgt tctgtctttt
      781 ctgtcattgc ttcttacatt tatgttaacc agatattata catatcttct ggtatgaaat
      841 aaatatttgt gatattattg ctccgttgta ttaatatttt aataacttaa atatatgcca
      901 ttaacagatc agccagtaaa aggaatgtat attaaagaag atgggagatt cttttttctt
      961 gaagatagaa agcttaaaac acaggggaga cagggaggac ttgtaattat gaagctcaaa
     1021 gcactagata atgggcagat aattaacaaa actgtaaagt ctggtacaaa ggttgagtat
     1081 attaatcctg aaacaaaaga agctcagtat ttgtataaag atgattcttt tgcatacttt
     1141 atggatacct caacatatga gactgtaaat atctcaaaag atgtaatagg gtcatatgta
     1201 aactttctaa aggagggtga ttctatactt atgcttacat atgagggtaa aatattaagc
     1261 attaaagaaa gcccttctgt tgtattggag gttactgtag cacaggatgc tgttaaggga
     1321 aatactgcaa ataatgcgac taaacttgtt acagtagaaa ctggctatca acttaaggtt
     1381 cctctattta tcaaaaaagg agataagata aagattaata ctgaaacagg agagtatagt
     1441 gggaaagcaa attaagagaa attaaggata atttctgata taattgctaa tgactttggt
     1501 gggatagctc agttggctag agcatgggat tcataaaccc aaggtcggtg gttcgatccc
     1561 acctcccact accatttcat ttgaaaatgt ataaatatgg aagaaaacaa aaaagaaaac
     1621 aaacagccta atgtaaagat tattagagtt actcctaaga agaggatgtc gcctgttatg
     1681 agttttgtaa tgtttctaat aatgtctttc cttttaacaa tagggattaa tgccattcta
     1741 ggatctacaa gtaaaggtca agaagttcct cttagcgagg ttgtctcata tatttcggag
     1801 ggggagtata aagatattgt tttaagagat gatatggtta tagttactca aagtgtaaag
     1861 tctgatggta aagatgttat aacaaagaaa tatgctttaa ttcctgttgg tactgatttt
     1921 tatggaatgt taagtgatgc tggagtagat attaaggcat taaaaaatga ttattatcag
     1981 cctcagcttg ggatttcaat aggggatatt atctccttta tacttttagc tgcaggactt
     2041 gttttagcat atatgcttat caaaaatatg cagaagtctg gtggacagat tatggatttt
     2101 ggacaaagca gagcaagact tctttttggg aagaagacag gagttacatt tgatgatgtt
     2161 gctggaatag atgaggtaaa ggaagagctt actgagattg ttgatttttt aaagagtcct
     2221 aaaaagtata tagacattgg agcaagaatt cctaagggag tattgttagt aggagctcct
     2281 ggtacaggaa agacactttt agctaaagct gttgcgggtg aggcaggtgt tcctttcttt
     2341 catacgtcag gttctgaatt tgaagagatg cttgttggag ctggtgcatc aagggtaaga
     2401 gatttgttta ataaagcaag gaaagcagct ccatgtatta tctttattga tgagattgat
     2461 gctgtcgcaa agaaaagagg taccgtgctt cactctggtg caggggagca gacgttaaat
     2521 cagattcttg tagagatgga tggacttgag ggtagagaga atgtgatagt acttgcggct
     2581 actaatagac ctgatgtttt ggatcctgct attttaagac ctggaagatt tgataggact
     2641 gttgttgttc ctatgcctga ttatcaagga agaaaggaga ttataatggt tcatgcaaag
     2701 ggaaagaagt ttgaagatag tgttgactta gatttaattg caagaaaaac aattggatac
     2761 tctggtgcag atttagagaa tcttttaaat gaggccgcta ttatggctgc tagagaaggg
     2821 aggaaaaaaa ttaatcaaaa tgatttaaca gaggcatatc ttaaagtcaa attgggtaga
     2881 aagagaaagg ggcagagtag tgaagatgat ctcaaggtta ctgcatatca tgaagcagga
     2941 catgctattg ttgcaaagtt tacagaacat gccactcctg ttgagcaagt ttccattata
     3001 cctaggggta tttcaggggg tgttactgta tatcttcccg aagatgataa aaagcatgta
     3061 tttaaagatg aacttcttgc aaatattaca tctgctgtag gaggcagagc ttctgaagag
     3121 cttttcattg ggagaatttc tactggtgca tctgcagata ttgaagaagc aacttctgta
     3181 gcaaaggaga tggtaacaca gtatggtatg tctgataagc ttggtttagt aaagtatggt
     3241 gacgtagaag aaacaaagca cttaggttat gtatatggag gcggaagaga ttttagtgaa
     3301 aagtatgcag aaatgattga ttcagaagtg aaaaatatta tggattctgc atatcatcag
     3361 gctaaaaaga tattatctga taagaaagaa tatgttgaaa aacttgtagc tctccttatt
     3421 aaaaatgagg ttgtctcaaa agaggagttt gatgcattgt ttaatgagta attgtagtta
     3481 aactctttct tttgtaaaat atattgatga ttaaagagtc tgagaaagat acatttcttg
     3541 ctattgatgc taatgccatt gtacatagag catttcatgc atatccatct acactccaaa
     3601 ctcctgatgg tgttcaagta aatgcagtat atggttttac cgtgatgctt cttgaagcat
     3661 ttaagatgtt tgaacctaaa tatgtattgt gtgcaatgga tacacataca ccaacattta
     3721 ggcatgaaat atatgtagac tacaaagcaa caagaaaacc cacagatcta tcattaatag
     3781 atcaatttcc attagtagaa gaaatattaa aagcatttaa tatcccagtt attaagaaag
     3841 aaggat
//
LOCUS       DAOSAB010000041         3837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_15902_length_3837_cov_2.629297, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000041 DAOSAB010000000
VERSION     DAOSAB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15902_length_3837_cov_2.629297"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK432_01220"
     CDS             complement(<1..211)
                     /locus_tag="PK432_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59579.1"
                     /translation="MREISYLQIEEIIKKSFILPIHSEDHIIYSLNNTLSKELKSRGK
                     KQYMLFVGLSIENIDWVIKETKIPSK"
     gene            570..2378
                     /locus_tag="PK432_01225"
     CDS             570..2378
                     /locus_tag="PK432_01225"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOK59580.1"
                     /translation="MKEFKKKNSVIVIMIMLLITKILGFWKLRVFAQLFGASHELDIF
                     WASFTIPDMIFMVLVAGSVNAAIIPILTDQLYDRGKKSLNELFKKITMYFFLLCFLFV
                     LIAFILAPTITEWIINSESASTILNIGFRMTQADYPLFLRLFRIGLLSPLFLSISAFV
                     TSYLQVRKQFFVTSLAPLFYDLAMIFGTYILVGIFKMDVTAIAISAVVGSVIHLAVQI
                     PTLNRFYEEDGNEDEVEIDEQQQKKEISKAFHLAIPRMLGVLGEQISTVVNTIISFTL
                     TAGALSAYKFANSLHFFPISIVGGAVAQVTLPDLAKFSNKKDEVSFKKVLNDSIQLSL
                     YLVFPIVAVLLVLRLPIVRLVYGTGAFDWQDTILTSWSLALLTGSIIGQTIELILLRA
                     FYALKNTWKPLISIFLGVVINLLGAYYLTNFFSHYYDWRPILEQIWIQISQANGEGVM
                     PVIHSFFKDTIKWMTTRGSSNYAVGGLALSLSLSYLFQVIISSLMLNSTKKVITWKET
                     VSPILKKIVNTLIMITGMYFLFKLFDFRLDTSRTVYVIILFIITSLGGLLLYLLGSKI
                     FSPREYDMAVDLIKRIWNRIKNLNKNGNGNGNNLKN"
     gene            2472..2984
                     /locus_tag="PK432_01230"
     CDS             2472..2984
                     /locus_tag="PK432_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59581.1"
                     /translation="MNWEKEFEKGINTTYVINQTSAESIDLGGKIAEFALSTDDVRSL
                     ELNVTEIGSVIYNTLDSYFGENINVERIYIQPSESRWVIYIDLRYKRFSVWVSVDINK
                     DNVQSAQLYITEIKVGPFDISDISDVSSKINKGIGEAMVTLNENGLVGRYIENIELLG
                     DFVILKGSRY"
     gene            3063..3365
                     /locus_tag="PK432_01235"
     CDS             3063..3365
                     /locus_tag="PK432_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOK59582.1"
                     /translation="MAENIKIQPLGSRVLIEPQEIQEKTPGGLVIPPNASDEKKPSYG
                     KVVALGKGKDSKGNLLKFNVKVGDMVYFKKYAPEEIEIDGKEWYILDTDDILAIAK"
     gene            3393..>3837
                     /locus_tag="PK432_01240"
     CDS             3393..>3837
                     /locus_tag="PK432_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOK59583.1"
                     /translation="MAKEIIYGEEARRALKAGVDIIADAVKTTLGPRGRNVALAKSFG
                     SQIVTKDGVTVAKEIEEKDPFKNVGVEMLKEAARKVNDTAGDGTTTVTVLAQAIANAG
                     FKNVTAGANPISLKRGLDKAVDIVVEELVKSSKKIKTQEEISQVAT"
BASE COUNT         1286 a          495 c          707 g         1349 t
ORIGIN      
        1 attttgaggg aatctttgtt tctttaatta cccaatctat gttttctata ctcaatccta
       61 caaatagcat atactgcttc tttccacggc tctttaactc tttgctaagt gtattgttaa
      121 gagagtagat aatatggtct tctgaatgga ttggaagtat aaaggatttt ttaataattt
      181 cctcaatttg tagatatgat atttccctca taagcaggta tttatatata gaagctattc
      241 ctttagatgc tttttgtttt agcttaatct cttgttccct agatatgttc catcctgtaa
      301 aacattggat tggtttgcta ctacactttt tatgtgtata atagccattg tttaatttgt
      361 ggcatatagg acaccgagga agagagtgtg gtatcttttt taagcagtta ctgcatatat
      421 cttctccaac cctggagcag aaaacacact gtttaggaaa gatattattt attagtagca
      481 taaaaatatt ttaatactaa aatgttaaaa agatttgaaa attaaaagct tttcaattat
      541 tatattaaag cttaaaccaa ctatcactta tgaaagaatt taaaaagaag aatagtgtaa
      601 tcgttattat gataatgctc ttgataacca aaatacttgg gttttggaag cttagagtat
      661 ttgcacaact atttggagca tctcatgaat tagatatctt ttgggcttca tttactattc
      721 cagatatgat atttatggtt cttgttgcag gttctgttaa tgcagctatt atcccaatac
      781 ttacagatca attatatgac agaggaaaga aaagtcttaa tgagttattt aaaaagatta
      841 caatgtattt ctttcttctt tgtttcttgt ttgttttaat agcatttata cttgcaccta
      901 ctattacaga atggataatt aatagtgaga gtgcctctac aatattaaat attgggttta
      961 gaatgactca agcagattat cctctttttc ttaggttatt tagaataggt ttactctctc
     1021 cactattttt aagcataagt gcatttgtaa cttcatatct acaagtgaga aaacagtttt
     1081 ttgttacatc actggcacct cttttttatg accttgcaat gatatttggt acatatattc
     1141 ttgttggtat ttttaagatg gatgttacag caatagcaat atcagctgtt gtaggatcag
     1201 ttattcatct tgcagttcaa ataccaacac taaataggtt ttatgaagag gatggtaatg
     1261 aagatgaggt agaaattgat gagcaacaac aaaagaagga aatatcaaaa gcatttcatc
     1321 tcgcaatacc aagaatgtta ggagtgttag gtgagcagat tagtactgta gtaaacacaa
     1381 taattagttt tactcttaca gcaggagcac tgagtgcata taaatttgca aacagtcttc
     1441 atttctttcc tattagtata gttggtgggg ctgttgccca agttacactt cctgatttag
     1501 caaaatttag caataaaaag gatgaagtaa gttttaaaaa ggtactcaat gattctattc
     1561 agctttcttt gtatcttgtc tttccaattg tagcagtact tctagtactt agacttccta
     1621 ttgttcgtct tgtgtatggt acaggagctt ttgactggca ggatactata cttacctctt
     1681 ggtctcttgc acttttaaca ggttctatca taggacaaac aattgagtta attttgttaa
     1741 gagcttttta tgctttaaaa aatacatgga agccattaat atcaatattc ttaggagttg
     1801 taattaattt acttggtgct tactatctta ctaatttctt tagccattat tatgattgga
     1861 gaccaatact tgaacagata tggatacaaa tatctcaagc taatggggaa ggagttatgc
     1921 ctgtaattca ctcgttcttt aaggatacta taaagtggat gactacaaga gggagttcaa
     1981 attatgcagt tggtggtcta gcattaagtt taagtctttc atatttattc caggttataa
     2041 tttcttcttt aatgttaaat agtactaaaa aagttataac atggaaggaa actgtaagcc
     2101 caattttaaa aaagattgtt aataccttaa ttatgataac tggtatgtac tttctgttta
     2161 agttgtttga ttttagatta gatacttcaa gaactgtata tgttattatc ctcttcataa
     2221 ttacctcact tggtggttta cttttgtatc ttttgggttc aaagatattt tctcctagag
     2281 aatacgatat ggcggtagat ttgattaaaa gaatatggaa cagaattaaa aacttgaata
     2341 aaaatgggaa tggaaatggg aataatttaa agaattaatg tatgaaaaag ttctttcaca
     2401 ccgttttaac actttttctt ttactaataa tctttctttt attgtttgta ggatatatta
     2461 gtttgagata tatgaactgg gaaaaagaat ttgaaaaagg tattaataca acctatgtaa
     2521 ttaatcagac tagtgcagaa agtatagacc taggagggaa aatagcagag ttcgcactat
     2581 caactgatga tgtaaggtct ttagagctaa atgttactga gataggttct gtaatctaca
     2641 atacattaga ttcatatttt ggtgagaata taaatgttga aagaatatat atacagccaa
     2701 gtgaatcaag gtgggtgata tacattgatt taagatataa aaggttttct gtttgggtat
     2761 ctgtagatat aaacaaagat aatgtacaga gtgctcagtt gtatataact gaaataaaag
     2821 taggtccttt tgatataagt gatatatcag atgtatcttc aaagattaat aagggtatag
     2881 gggaggcaat ggttacatta aatgaaaacg gtctcgtagg aagatatata gaaaatattg
     2941 agctccttgg tgattttgta atcttaaaag gttcaagata ttgacatttg gcttactgct
     3001 gattaaaata cttagcagtc ttgcttgtaa attgctaatt taatttgttt taattaatta
     3061 atatggcaga aaatataaag attcaaccat taggcagcag agtgttgatt gaaccacaag
     3121 agatccaaga aaaaactcct ggtggtttag ttataccccc aaatgcttct gatgagaaga
     3181 aaccttcata tggtaaggtt gtagcacttg gtaagggtaa agattctaaa gggaatcttc
     3241 ttaaatttaa tgttaaggta ggagatatgg tttactttaa gaaatatgct cctgaagaga
     3301 tagagataga tggaaaggag tggtacatac ttgatactga tgatatttta gctattgcta
     3361 aataactaga taatttgtaa ttcttaaata atatggcaaa agaaataata tatggagaag
     3421 aagctaggag agcacttaaa gcaggtgttg atataattgc tgatgctgtt aaaacaacac
     3481 ttggacctag aggaagaaat gtcgcattag caaaatcttt tggttctcaa attgttacta
     3541 aagatggtgt aacagtagca aaagagattg aagaaaagga tccttttaag aatgttggcg
     3601 tagagatgct taaagaggct gcaagaaaag taaatgatac tgcaggtgat ggaactacca
     3661 cagtaactgt tttagctcaa gctattgcta atgcaggttt taaaaatgtt actgctggtg
     3721 ccaaccctat ttctcttaaa agaggtttgg ataaggctgt agatattgtt gtagaggaat
     3781 tagttaaatc ttctaaaaag ataaagacac aggaggagat ttctcaagtt gctacaa
//
LOCUS       DAOSAB010000042         3782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_16220_length_3782_cov_3.059834, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000042 DAOSAB010000000
VERSION     DAOSAB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16220_length_3782_cov_3.059834"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PK432_01245"
     CDS             <1..1184
                     /locus_tag="PK432_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOK59584.1"
                     /translation="QSESNSQLISNAEKELRKLTEEETKRLEEIKAEITELENKSNNQ
                     IKTKKTMETKNFSLINTIRNVVEGRQQDDLSLEVMQKGAEELRKAGVTYKGQITLPFE
                     YRSTLVSASNPAVETSVLDIQTPLYNALTVTKAGATILNGLVGNVVIPTYTGSNVAWK
                     SELDAATDGTGTMGSVDLKPKRLTGYFDVSKMLLAQDSVGVEQMLRNDIVNSIAVAIE
                     KAIFSKIVDDNAPKGFFAGFNADTNFDVAGELSWEKVVELESKVDTSNALMGNLAYIT
                     HPSLKGLAKTTPKASNTAVFVAEGNTINGYNLLSTSSMATNITTKNYYGIIFGNWADL
                     LIGQWGGVDIVVDPYTQAGNGKIRIYVNAYFDAKPRRKESFAIGGLKEAEVVPPSTGG
                     N"
     gene            1291..1578
                     /locus_tag="PK432_01250"
     CDS             1291..1578
                     /locus_tag="PK432_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HOK59585.1"
                     /translation="MTTILDELKDYLNIESSDTDKDALLSTILKVSETAIKNYCRGGL
                     DGVVIDENNVLRFAKLQYAAHLFLNRGVVSIGQTYELPLTFKFLLNPYVKY"
     gene            1584..1898
                     /locus_tag="PK432_01255"
     CDS             1584..1898
                     /locus_tag="PK432_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HOK59586.1"
                     /translation="MNVGLMRDKIKILRKTVKKDEYGSEVETYTKLYEPYAYIKYLNG
                     NKRISASEVFYNQTLNITIYYKNLNTTDMVEFNGSKYRIIDIVPSTDLIFISMVCERI
                     ND"
     gene            1928..2413
                     /locus_tag="PK432_01260"
     CDS             1928..2413
                     /locus_tag="PK432_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59587.1"
                     /translation="MLTLNDKALKFFLKEMQPKYIKRVGYSALMAAGRKVVNVAKRGY
                     KSTVKGYSKSNNQNVLKSFVVRKSKREVAVWVGSSYYKTRWLEKGTKERYTKGYKRKS
                     TMGKRQLSKTKLYRGSITGSGFFEKAYNSVQGELKEVITQRLQQNINKVIEKANSNAK
                     N"
     gene            2400..2702
                     /locus_tag="PK432_01265"
     CDS             2400..2702
                     /locus_tag="PK432_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOK59588.1"
                     /translation="MPRIELKPYKTYNDKKVYRSKGNNLIHQLVYNTGRWREMRVMYL
                     MKQPICEVCKKEFAVEVHHKIPISTGVDRSEILKLGFDADNLMALCSNCHRRIHND"
     gene            2833..3174
                     /locus_tag="PK432_01270"
     CDS             2833..3174
                     /locus_tag="PK432_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59589.1"
                     /translation="MISALEINKEIATILKGLNYKHYPLVIPEDTQMPVIVYNRTVDY
                     SDDNSDTTIGVATIELEVLTGNYKDGIDILNKLYSSLLGKGYSPEGITEDFIDGVYIQ
                     KLVVSKYVLIN"
     gene            3205..3651
                     /locus_tag="PK432_01275"
     CDS             3205..3651
                     /locus_tag="PK432_01275"
                     /inference="COORDINATES: protein motif:HMM:NF017961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HOK59590.1"
                     /translation="MATILGSDLMVFVDNKPIAFSTSAKVSVNANAEDISSKDSGLWE
                     ESLITKFSWTVSTDALYSIDGAGTGNSDLSELWTSFVGKTKVSVAFAIKDGTSPNWTK
                     KTGSKAFSGNGYIVKLDANADQKGVATYSIEIKGDGELQLATVTSS"
BASE COUNT         1368 a          508 c          767 g         1139 t
ORIGIN      
        1 tacaatctga aagcaattca caacttattt ccaatgcaga aaaagagctt agaaagctca
       61 ctgaggaaga aaccaaaagg ttggaagaaa taaaagcaga aataactgag ttagaaaata
      121 aaagtaataa ccaaattaaa acaaagaaaa ctatggaaac aaaaaatttt tcattaatca
      181 atacgattag aaatgttgtt gaaggtcgcc agcaagatga tttatcactg gaagtaatgc
      241 aaaagggtgc agaagagtta agaaaggctg gagttaccta taaaggtcaa attaccctac
      301 cttttgagta ccgttcaact ttagtttcag caagcaaccc agccgtagaa accagtgtat
      361 tagacattca aactccactg tacaatgcac ttactgttac taaggctggt gcaaccattc
      421 ttaatgggct tgtgggtaat gtagtaatac ctacctatac tggctcaaat gtggcttgga
      481 agtctgaact agatgctgca actgatggaa ctggtactat gggttctgta gacctcaaac
      541 ctaaaaggtt aactggttac tttgatgtat caaagatgct tcttgcacaa gattctgtag
      601 gggtagagca aatgcttaga aatgatattg ttaactctat tgcagttgct atagaaaaag
      661 caatcttttc aaagattgtt gatgacaatg cacctaaagg attttttgct ggttttaacg
      721 ctgatactaa ttttgatgtt gctggagaat tgagttggga aaaagtagta gaattggaaa
      781 gcaaggtaga tacttctaat gccttaatgg gtaaccttgc ttacattact cacccttcac
      841 ttaagggact tgccaagacc actcctaagg catccaacac cgctgtattt gttgctgagg
      901 gtaatactat caatggttac aacctacttt ctacttcttc gatggctacg aatataacaa
      961 ctaaaaatta ctatggtatt atctttggta actgggctga tttactaatt ggacagtggg
     1021 gtggtgttga tattgttgtt gacccttaca cccaagctgg taatggtaag attcgtatct
     1081 atgttaatgc ttattttgat gctaaaccca gacgtaaaga aagttttgct attggtggct
     1141 taaaagaggc tgaggttgtt cctccttcaa ctggtggaaa ttaggctaaa ttttgattga
     1201 tttactaata ggtggtgtta ctatatggta gcaccacttt tttttgatta aattaaaaat
     1261 tttggtgtta atatagtaaa aggggtaact atgacaacta tactagatga actaaaggat
     1321 tatttaaata tagagagttc agacactgat aaagatgcac tgctatccac tatactcaaa
     1381 gttagtgaaa ctgcaattaa gaactactgt aggggtggtt tagatggtgt tgttattgat
     1441 gaaaataatg tattgagatt tgccaaatta cagtatgcag cacacctatt cctaaatagg
     1501 ggtgttgttt ctattggtca aacctatgag ttaccactta cctttaaatt tttacttaac
     1561 ccatacgtaa aatactgatg gttatgaatg taggtttaat gcgagacaag ataaaaatac
     1621 taagaaaaac agtaaaaaag gatgagtatg gctctgaagt agaaacctat accaaactct
     1681 atgagcctta tgcttatatt aagtatctta atggtaataa aaggataagt gcaagtgaag
     1741 tattttacaa ccagacactt aacattacta tctactataa gaaccttaat acaactgata
     1801 tggttgagtt taatggttca aagtatagaa ttatagacat agttccttct actgatttga
     1861 tttttatttc aatggtttgt gagagaataa atgactaata caaataccca attctaaacg
     1921 ttaatatatg ttaacactga atgataaagc actgaaattc tttctgaagg aaatgcaacc
     1981 taagtacata aaaagggttg ggtatagtgc tttaatggca gctggaagaa aggtagttaa
     2041 tgttgctaaa agggggtata agtcaactgt aaaaggttac tcaaagagca ataaccagaa
     2101 tgtactaaaa agctttgttg ttaggaagtc aaaaagggaa gttgctgtgt gggttggttc
     2161 tagttactat aagaccagat ggttggaaaa agggactaag gaaagatata ccaaagggta
     2221 taaaaggaaa agtacaatgg gtaaaaggca actaagtaaa actaaactgt ataggggttc
     2281 aataactggt agtggtttct ttgaaaaagc atataattca gtacaaggtg aattaaagga
     2341 agtgattacc caaaggttac aacaaaatat aaataaagtt attgaaaaag ctaatagcaa
     2401 tgccaagaat tgaattaaaa ccatataaaa cctacaatga caaaaaggtg tataggagta
     2461 agggtaataa tcttatacat caattggtgt ataatactgg tagatggaga gaaatgaggg
     2521 taatgtacct aatgaaacaa ccaatttgtg aggtgtgtaa gaaggagttt gctgtggagg
     2581 ttcaccacaa aatacccata tcaactggtg tggatagaag tgaaatactt aaactgggtt
     2641 ttgatgctga taacttaatg gcattatgct ctaactgtca taggagaata cacaatgatt
     2701 aaaaaaacta tagggatgca aacttggtta taagtagtgg acaaaactat ataatgggag
     2761 aaatatagaa gattatatct tactatatac actaataaga gtaagtaata acttaaatga
     2821 atagatatgg atatgataag tgcattagag ataaataagg aaatagcaac tatactgaaa
     2881 gggttaaatt acaaacacta tccattggta ataccagaag atacacagat gccagtaatt
     2941 gtgtataata ggacagttga ctacagtgat gataactcag atacaacaat tggtgtagca
     3001 accattgagt tagaagtact aactggtaat tacaaggatg gtatagatat tctaaataaa
     3061 ctatactcat cattactggg taagggttat tcaccagagg gaattactga ggatttcata
     3121 gatggtgttt acattcagaa gttagtggta tcaaaatatg tgttaattaa ctaaataagt
     3181 ataacaattt aaaacaataa aactatggca acaattttag gttctgattt aatggttttt
     3241 gtagataata aacccattgc attttctaca agtgccaaag taagtgtaaa tgcaaatgct
     3301 gaggatattt ccagtaagga tagtggacta tgggaagaaa gtttaatcac taagttcagt
     3361 tggactgtta gcacagatgc tttatatagt attgatgggg ctggtactgg taacagtgat
     3421 ttaagtgaac tttggacttc ctttgtaggt aaaactaagg taagtgtagc atttgctata
     3481 aaagatggaa cttcaccaaa ctggactaaa aagactggta gcaaggcatt tagtggtaat
     3541 ggttacattg taaaattaga tgccaatgct gaccagaaag gggtagcaac ctattccatt
     3601 gagattaaag gtgatggtga attacaactt gccactgtta catcatctta attattgttg
     3661 tgaattgctt tcagatgaat ggtagtctat agttaggcta ccattttttt tttttgtaat
     3721 attttcatct taatatcaaa aaattggtgt taatataatt aaagagtagt gctatggaag
     3781 aa
//
LOCUS       DAOSAB010000043         3707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_16620_length_3707_cov_2.635268, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000043 DAOSAB010000000
VERSION     DAOSAB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16620_length_3707_cov_2.635268"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..377
                     /locus_tag="PK432_01280"
     CDS             21..377
                     /locus_tag="PK432_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59591.1"
                     /translation="MAEKKIIDILEILSVLLGVFIIFKLVSKQKKSPKEEEEDNLNHR
                     FVSSGKSLNERQRGILKLMQKKKVVLPSQIYDLYPEVSTRTLRRDMDVLVELGFVRQE
                     GSTRDTKYILNNHIYA"
     gene            507..1157
                     /locus_tag="PK432_01285"
                     /pseudo
     CDS             507..1157
                     /locus_tag="PK432_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
     gene            1225..3072
                     /locus_tag="PK432_01290"
     CDS             1225..3072
                     /locus_tag="PK432_01290"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOK59592.1"
                     /translation="MHKYIEVCNLFEKLELTPSRNEMSQELARFLKDCNKEEGQIISY
                     MIQGRVAPMFVNSEFNYSERSLVKVLTNQYNIDALKMRKEYGDIGDTVYEILEQNSNK
                     GGIFDLISLYEILWKIVNTSGSGSMLEKNRIILDCLKNLSPIEGKYFVRTICGELRLG
                     LSMRSLLDVLSIITVGDKSLKPLLEHAYGICPDIGYISSNIIGYGESDVRKNLSSVHV
                     TPGVPILSRLVQRVGSFDELTERFTGTVLLQPKFDGLRCQIHKWNKKDLVMTQKDVVW
                     KKYKKEDTFPSPSLFENEKISDVSVKLFTRNLEDVTKMFPEIVDSARKIQSESFIFDS
                     EVVGWNYKTERFMSYQETMQRRRKYSVQSKSIDIPVRAFVFDLLYLNGNSLINIDTEK
                     RIEMLEKNIKSSKEGSIALAESKKVSSEKEIKKYFKECIKNGLEGIIVKEIKGGYKPG
                     IRNYEWVKIKKSIDKKLVDTVDMVVVGYYSGSGRRSSLGVGAILGAVLNENEGTFDAI
                     CKVGTGMGDELLKDMYSKFQDITVKQKPKDVRVVAFLIPDVWVSPVYVVTVDADEITR
                     NISGTNASIGNGLSLRFPRLIDFGRDKSPEDITTVKELSDMFRIQKKKK"
     gene            3265..3660
                     /locus_tag="PK432_01295"
     CDS             3265..3660
                     /locus_tag="PK432_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59593.1"
                     /translation="MLIGEFERIKQDPKNLLSNKEITIEPDSFNPIMVKIDEERKLLV
                     IKFPTPIETAEVAYVAILLNAENPRYFTLELHRPNEMEKQMFPDRTYDRYEFCEWSKD
                     GKHHILGTKNGELKEPNPDIFIEEIKAIL"
BASE COUNT         1411 a          413 c          762 g         1121 t
ORIGIN      
        1 aaaataatta atgtaaatat atggcagaaa agaaaataat agacatatta gagatattat
       61 ctgttctact tggtgtgttt atcattttta aactagtttc aaaacagaag aaatcaccta
      121 aagaagagga agaagataac ttaaatcata ggtttgtttc aagtggtaaa agtctaaatg
      181 aaagacagag aggtattctt aaattaatgc aaaagaagaa agtagtactt ccatcacaga
      241 tatatgacct gtatcctgag gtgtcaacaa ggacactgcg aagagatatg gatgtactag
      301 tagaacttgg gtttgtaagg caggagggta gtacaagaga tacaaaatat atattaaata
      361 accatatata tgcataaaga tattgaagtt aataatgtat tgtaatagta ggatatgata
      421 gaataataag aaggaatgga tgaaaaaata ttgataataa tagtaaataa aaagaatatt
      481 atttaataga tatcatgaaa gattctaatg gagttacaat tattgaccca gaaaccacat
      541 atatcgaacc gaatgtaaaa attggattag gtacaataat ttatccagga gtaataattg
      601 aagagaattc aactattggg aaaaattgtg taattagagg aaatacaagg attattaata
      661 gtgtaattgg aaatgattgt gaaatagaat cagcagtaat tgaatcaagt actgtaggga
      721 acaattgtca tattggtcca ttcgcccatc taagacctaa ctgtaagctt ggcaataata
      781 ttaaaacagg aaattttgtt gaaataaaga actctactat cggtgataat acaaaagcag
      841 cccatcttgc atatgttggt gatgcagata ttggtaaaaa tgtaaatata gggtgtggag
      901 tagtttttgt taattatgat ggcaagaata agtttcgttc tactgtaaaa gacaatgctt
      961 ttataggaag taattcaaat ttagtagctc cagttgttat agaagaatat ggctatgtag
     1021 cagcaggttc aaccataact gacaatgtag gtaagggaga tctttcaata gctagagcaa
     1081 ggcaggtaaa tatcccaggg tgggttgaga aaaagggcct taggaatcat aaacaaccaa
     1141 agggatattg taaatgaacc ttcgctatga aattaaagtt tttaagggag caagagatac
     1201 aaaatatatt aaacaaccat atatatgcat aaatatattg aggtatgtaa tctgtttgaa
     1261 aaattagaat taacaccatc aagaaatgag atgtcgcagg agctggctag attcttaaaa
     1321 gattgtaaca aggaagaggg acaaattatt tcatatatga tacaggggag ggttgctcct
     1381 atgtttgtaa atagtgagtt taattactct gaaagaagtt tagtaaaagt acttacaaat
     1441 cagtacaata ttgatgcatt aaagatgaga aaggagtatg gagatatagg ggatactgta
     1501 tatgagatat tagaacaaaa ttctaataaa ggcggtattt ttgatttaat ttctctttat
     1561 gaaattcttt ggaagattgt aaatacatct ggttcaggat ctatgttgga gaagaataga
     1621 attattcttg attgccttaa gaatttaagc cctattgaag gaaagtattt tgttagaact
     1681 atttgtggtg aattaagact agggcttagt atgaggtcac tgcttgatgt tttgtcgatt
     1741 attacagtag gggataaaag tcttaaaccg ctgctagagc atgcatatgg gatatgtcct
     1801 gatattggat acatttctag caacatcata ggatatgggg aatcagatgt gagaaaaaat
     1861 ttatcatctg ttcatgttac tcctggagta cccatattat ctaggcttgt acagcgtgtt
     1921 ggaagttttg atgagcttac agaaaggttt acaggaactg tacttttaca acctaaattt
     1981 gatggcctta gatgtcaaat tcataaatgg aataaaaaag atttagtaat gactcaaaag
     2041 gatgtagtat ggaaaaagta taaaaaagaa gatactttcc catctccgag tttgtttgaa
     2101 aatgaaaaaa tatcagatgt aagtgttaaa ctttttacaa gaaatttgga agatgttacg
     2161 aaaatgtttc ctgaaattgt tgatagtgca aggaaaatac aaagtgagtc ttttatattt
     2221 gattcagagg ttgttgggtg gaattataaa acggagaggt ttatgtcata tcaagaaaca
     2281 atgcaaagga ggaggaaata ttctgtacag agcaaaagta tagatatccc tgttagggct
     2341 tttgtatttg atcttcttta tcttaatgga aactctctta ttaatataga tactgaaaag
     2401 aggatagaaa tgcttgaaaa gaatattaaa agttcaaagg aggggagtat agcattggct
     2461 gaaagtaaaa aagtttctag cgaaaaagaa ataaagaaat atttcaaaga gtgtattaaa
     2521 aatgggttag agggaattat agttaaggag ataaaaggag ggtataagcc aggaataaga
     2581 aattatgagt gggttaaaat taagaaaagt attgataaga aacttgttga tactgtagat
     2641 atggttgttg ttggatatta tagcggttct ggaagaagat cctctcttgg tgttggtgct
     2701 attcttggtg ctgtcttaaa tgaaaatgag gggacgtttg atgcaatatg taaggtaggt
     2761 actggaatgg gtgatgagtt gcttaaagat atgtattcta aatttcagga tattactgtt
     2821 aagcaaaagc caaaggatgt tagagttgtt gctttcttaa ttcctgatgt atgggtgtct
     2881 cctgtatatg tggttactgt tgatgctgat gaaataacta ggaatatatc aggtactaat
     2941 gcatcaatag ggaatggtct atcattaagg tttcctaggt taattgattt tggcagagat
     3001 aaaagcccag aagatattac tactgttaaa gaattgagtg atatgttcag aattcaaaaa
     3061 aagaagaaat aggtaatgta cagtttacaa ttgacactgt gtagcagtat atgtaaactt
     3121 taatattaat ttaaacattt ttcttagatg gacaaaccct ctctgctcta ttattatgca
     3181 cacatattct taaagcatat tatttttaat atgtgggatg attttaataa aatcataaac
     3241 caaggaccta atgcattaaa aaatatgctt ataggcgaat ttgaaagaat taagcaagac
     3301 ccaaaaaatc tactatcaaa caaagaaatc acgattgaac cagacagctt taatccaata
     3361 atggttaaga ttgatgaaga gaggaaactt ctcgttataa aattcccaac tccgatagaa
     3421 acagcagaag tcgcatatgt agcaatatta ctgaacgcag aaaatcctag atactttact
     3481 cttgaacttc atcggccaaa tgaaatggaa aaacagatgt tcccagatag aacatatgac
     3541 agatatgaat tttgtgaatg gagcaaagat ggtaaacacc atatattagg aacaaaaaat
     3601 ggagaattaa aagagccaaa ccctgatatc tttattgaag agataaaagc tatactctaa
     3661 aagcatataa agagtaagga atggaggagt ttaacaatct ttttcct
//
LOCUS       DAOSAB010000044         3660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_16901_length_3660_cov_3.999445, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000044 DAOSAB010000000
VERSION     DAOSAB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16901_length_3660_cov_3.999445"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PK432_01300"
     CDS             complement(<1..515)
                     /locus_tag="PK432_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59594.1"
                     /translation="MTEFGSDERMSLDDDSYFINPEDFGDLRDLKIEKIPPYEGEGKM
                     SFSSKYDREKVEYLNSIGVDVPKEWFDHNGEIIENCRPLATTMFIVTGHILAMVMAER
                     IIEGDPGYKERFTQLVKGSNAFLLENRLDTSGRRIEVLYGVLFEDIKRGLGFSANPEQ
                     RVSKEELHDIV"
     gene            complement(755..1255)
                     /locus_tag="PK432_01305"
     CDS             complement(755..1255)
                     /locus_tag="PK432_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59595.1"
                     /translation="MSIEDNYGNYEIDVTAKFPAFLIDNNEEGYTLFSWEDEKGFEKI
                     PSDRAFTIEGKGLSDDDESLLFQLSYKESKSSQANRDLWDIPINKKEVYEELVKKGLE
                     IVKEVASSDDKIAISSFSKEVLLDGKTYVVTYMAANKYIAGISMRDNYVRRIKERDSK
                     KKRGTN"
     gene            complement(1365..1982)
                     /locus_tag="PK432_01310"
     CDS             complement(1365..1982)
                     /locus_tag="PK432_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOK59596.1"
                     /translation="MVKSDKLNVDAYAFVFKEMFNIDFTRDDFMKYISGRGSREGIIE
                     YLASNGITEFSSQELNDLFYKYKNKLVSEKMNSEIYLLPGIRDFLKNREIMNKRKVII
                     TSSRKAHVKNILTHFGIYTYFEKVIDRFDVVKGKPSPEAYLKGLEYLGCTETECVAFE
                     DSFFGLQSAKGAGIFTIGILNRGWNDDFVYQLSDYVIDDYNKLIV"
     gene            complement(2080..3087)
                     /gene="trpS"
                     /locus_tag="PK432_01315"
     CDS             complement(2080..3087)
                     /gene="trpS"
                     /locus_tag="PK432_01315"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOK59597.1"
                     /translation="MKKNEYKVHVFTGIRPSGGLTIANVIGSVNTIVSLQKEKKIERP
                     MVFVADLHAITDAKPEDTQKYVVDIIKDYFALGLDKDNCDVFIQSHLIEEISELNLYL
                     SRLISISELLRVPTLKEKIKRNIDVSNASLLLAMYPVMMSCDILLQKAEYVPVGEDQS
                     AHIEMARYLAKKFNKEHGEVFPIPKMLSLGEPVRIMSLIGEGKMSKSNPNGAILLDDP
                     VNVSLNKVKRAQSAFAGEMSESLDSLIKIGKFVSDESGQKEIDELVSKHMNGEGVMKD
                     LKEVIMESLRKYLTDFQEKKSQMSDSEVLEIAKRGGMIAKKNAKETIQEARNAVGLKY
                     I"
     gene            3134..>3660
                     /locus_tag="PK432_01320"
     CDS             3134..>3660
                     /locus_tag="PK432_01320"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOK59598.1"
                     /translation="MKLEDFKSLKEIHEYFALHNSCKLKKYATQPVYELTPPKSGIVF
                     IGEAPGVNEDKQGKPFVGAAGKLLDEMLRSIGYTREDVYVTNVVKYRPPENREPLPEE
                     KEACRIWVNAGLLFIKPKVIVALGRHALERFLPQEHVSIAHGQVFIHSSGIPVFAMYH
                     PAAALYNPTLKETLQ"
BASE COUNT         1244 a          769 c          428 g         1219 t
ORIGIN      
        1 ttaactatat catgaagctc ttcttttgag actctctgct caggattagc tgaaaaacct
       61 aaacctctct ttatatcctc aaacagaaca ccatacaaca cctcaattct tcgcccacta
      121 gtatcaagtc tgttctccaa tagaaaagca ttagaaccct taaccaactg cgtaaatctc
      181 tctttatagc caggatctcc ctcgattatt ctttctgcca tcaccattgc aagaatatgc
      241 ccagttacta taaacatagt agtagcaagc ggtcggcagt tctctattat ttctccatta
      301 tgatcaaacc attcttttgg tacatcaact ccaatactgt ttaaatactc aaccttctcc
      361 ctgtcatatt ttgatgaaaa tgacatttta ccttcccctt catatggggg aattttttca
      421 atttttaaat ctctaagatc tccaaaatct tctggattaa taaaataaga atcatcatct
      481 aatgacattc tttcatcact tccaaactct gtcatataaa gacatattaa ttaatttaac
      541 cctatattat acaatatcct tctgatatcc tgaataacca ttattaagat ataccaaata
      601 tctagataat atttaatcag aaatataaat gaaaaatttt tgtaataatc ttttttacaa
      661 actataaaag tataaatagt ctattccaga tggaaagctc atttcataaa cacgatactt
      721 cttacacaga aacagaattg ctctccctaa aagactaatt tgtacctctt ttcttcttag
      781 agtccctttc ttttatcctt cttacataat tatctctcat agagatacct gctatatact
      841 tattagcagc catatatgta acaacatatg ttttaccatc aagcaaaact tcttttgaaa
      901 aagaagatat agcaatttta tcatcagagg atgcaacttc ttttacaatt tccaacccct
      961 ttttaactaa ttcttcatat acttcttttt tattaatagg gatatcccac aaatctcgat
     1021 ttgcctgtga gctcttactt tccttgtaac ttaactgaaa aagaagagat tcgtcatcat
     1081 ctgacaagcc cttcccctct atagtaaatg ccctgtctga agggattttt tcaaatcctt
     1141 tctcatcctc ccaagagaaa agagtatatc cctcctcatt attatcaatt agaaaggcag
     1201 gaaatttagc tgtaacatct atttcataat ttccataatt atcttcaatt gacatagaaa
     1261 tatcataaaa tattaaagca atatcatacc atatttagcc gtagtattta actctgttat
     1321 ttacaaattt cttcaatgta taggttatgt acagatatct tagtctagac aatcaattta
     1381 ttataatcat ctataacata atcagaaagc tgatatacaa aatcatcatt ccacccccta
     1441 ttaagtattc caattgtaaa tattccagca cccttagcag attgtaaacc aaaaaaagaa
     1501 tcttcaaatg caacacactc tgtttctgta cagcccaaat actccagccc cttaagatat
     1561 gcttcaggac taggtttccc tttaacaaca tcaaatctgt caataacttt ctcaaagtat
     1621 gtatatatcc caaagtgggt aagaatattc ttcacatgag cctttcgaga agaagtaata
     1681 attacttttc tcttattcat aatttcccta ttctttaaaa aatctctaat ccctggaaga
     1741 agatatatct cactattcat cttctcacta actagtttgt ttttgtattt ataaaataaa
     1801 tcatttaatt cttggcttga aaactctgta ataccattag aagctaaata ctctattatc
     1861 ccttcccttg atcctcttcc tgaaatatat ttcataaaat catctctagt aaaatcaata
     1921 ttaaacattt ctttaaacac gaaggcatat gcatcaacat ttagtttatc agatttaacc
     1981 attgaacaat caaattaaaa cataaccctt ttgaattcaa tatcctttct tttacatctt
     2041 caattgaatt gatcataata ttgtattaga aagaagtaat tatatatact tcaaaccaac
     2101 agcatttctt gcttcctgta tagtttcttt agcattcttc tttgcaatca ttccacctct
     2161 tttagctatt tctaaaacct cagaatcaga catttgtgat ttcttctctt gaaaatctgt
     2221 cagatacttt cttaaagact ccattattac ctcctttaaa tctttcatga caccctctcc
     2281 attcatatgc ttactcacca gttcatcaat ctccttctgt ccgctttcat cagaaacaaa
     2341 cttccctatt ttaatcaatg aatccaaaga ctctgacatc tcccccgcaa atgcactttg
     2401 tgctctcttt actttattca atgaaacatt caccggatca tcaagcagta ttgctccatt
     2461 aggattgctc ttactcattt taccctcgcc aatcaatgac ataatcctta caggctcacc
     2521 caatgaaagc attttaggta ttggaaatac ctctccatgc tccttgttaa acttctttgc
     2581 taggtacctc gccatttcaa tatgagcact ctgatcctct ccgactggaa catactctgc
     2641 tttctgaagc agaatatcac aagacatcat aactggatac attgcaagaa gcaaagaagc
     2701 attagataca tcaatatttc tctttatttt ttcttttaga gtaggcactc ttaaaagctc
     2761 tgaaatacta attaacctag aaaggtatag attaagctca gaaatttctt caatcagatg
     2821 agattgtata aacacatcac aattatcttt atcaagacca agagcaaaat aatccttaat
     2881 aatatcaacc acatactttt gagtatcctc aggttttgca tcagtaattg catgaagatc
     2941 tgctacaaaa accataggtc tttctatctt tttttctttc tgtaaagaca caatagtatt
     3001 aacagaacca attacatttg caatcgtaag accacctgat ggccttatac cagtaaaaac
     3061 atgtactttg tattcattct ttttcatatt gctaattata cactaaacaa taaattaata
     3121 tataataaaa gagatgaaac ttgaagattt taagagccta aaagaaatac atgagtactt
     3181 tgcactgcat aacagctgta aattaaaaaa atatgcaaca caacctgtat atgaactaac
     3241 accacctaaa tcaggcatag tatttattgg agaagcacct ggagtaaatg aagataaaca
     3301 aggaaaacca tttgtaggtg cagctggaaa gcttttagat gaaatgctaa gaagtattgg
     3361 atatacaaga gaagatgtgt atgtaacaaa tgtagttaaa tacagacctc cggaaaacag
     3421 agaacctctg cctgaagaaa aagaagcgtg cagaatatgg gtaaatgcag gacttctctt
     3481 tatcaaacct aaagtaatcg ttgctctcgg aaggcatgct cttgaaagat ttcttccgca
     3541 ggaacatgta tcaatagccc atgggcaagt atttatacac agcagcggca tacctgtatt
     3601 tgcaatgtat catcctgcag cagctcttta caaccctacc ctaaaagaaa cactacaaga
//
LOCUS       DAOSAB010000045         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_17144_length_3623_cov_3.768498, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000045 DAOSAB010000000
VERSION     DAOSAB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17144_length_3623_cov_3.768498"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PK432_01325"
     CDS             <1..617
                     /locus_tag="PK432_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOK59599.1"
                     /translation="GHTFDLADDWKEIDFVEAIKENFNVDIVNDNEESMLKALKDKGL
                     EVEEGMNRNRMTDSMWKYVRSSISGPAFLVNHPMFVSPLAKSKEENQNITERFQVIIA
                     GSELGNGYSEINDPQDQLMRFLEQQKLRESGDEEAQMLDIDFVEMLEYGMPPVSGYGQ
                     SERVFWFLENITAREGTLFPQMKPELEQSTKKIYKDILPKVKKK"
     gene            618..977
                     /locus_tag="PK432_01330"
     CDS             618..977
                     /locus_tag="PK432_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59600.1"
                     /translation="MDSIKDIFTKLKDNQSKKKSQQELFDTDEYSKKRVMLNDRHKYI
                     SKEYQYYGLHLAGKLGDKKRATMYIKWAKEKPRVILEKAYSFAIDYPNAKDRSRIFMW
                     KVKELEEQMKGEKKVES"
     gene            complement(928..1962)
                     /locus_tag="PK432_01335"
     CDS             complement(928..1962)
                     /locus_tag="PK432_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOK59601.1"
                     /translation="MKEEYKKLKKLIEKSNNILIITHRGPDFDAFASSLILKNYINTF
                     YPKKNVVFKTKQNPSQKIPLMEEITIVQEFNTENEELVIIMDAGGWGMCVSKGDTIQV
                     TKAKVAVVDHHITKGIQADVVINDQMSSTTEQILDFCMSVQKRYKITPTVSILGQIGI
                     VYDTGRFAYDNTTPHTYEMMAKLTSVYRLNIEEFEYKSAKFPASSLTPIKVYIENIHI
                     VGDMAYTYINKKDIESLGITQIGINGAYEFVRDNIIRYIQGVHWGFIIKPSSTAQNEW
                     KVSFRSSKDYQEVATMAEALGGGGHQYASAAVVQAEEGDKASEIVLGVVNKVLSSQPS
                     SHPSSVLQAL"
     gene            2049..3320
                     /gene="serS"
                     /locus_tag="PK432_01340"
     CDS             2049..3320
                     /gene="serS"
                     /locus_tag="PK432_01340"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOK59602.1"
                     /translation="MIDIKRIVENKEEVEKALSKRMKVEDMHLDEIIDLYNKRKEVLK
                     EYESKRAQQNSFNSKMAGIEKGSEEFKKLVSDLKTLSQNVKELERNVDELTQKLTSLV
                     EVLPNIPDEDVVAGEKEANQVIKVVGEKPIFNFPIKDHVELGESLGLFDLERAVKISG
                     NNFVMYKGWGARLEWALLNYFINSHLSDGYEMILPPHIVGERSGYTAGQLPKFKDDVY
                     WLKDLDQFLIPTAETALDNLFRDEILNEKDLPKKYFSYTPCYRKEAGSYRANEKGLIR
                     VHQFNKVEMYQYTTQEGSDLAFEELLGKAEKLVADLGLHYRISKLAAGDCSAPAARTY
                     DVEVWLPAVSQYYEVSSISNVRDYQARRGNMRYRKEDGTIEYMHTLNASGLATSRLMV
                     ALVETYQQEDGSILVPDVLRKYIGVDKIEKK"
     gene            3322..>3623
                     /locus_tag="PK432_01345"
     CDS             3322..>3623
                     /locus_tag="PK432_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOK59603.1"
                     /translation="MSYFLAFVPDKESVKEIQNVVKEANSLFSDFGIPVRWVKPDTYH
                     ITLYYLGENYPIFNQMLLKKKLSKIKFREFKISFQNIKVGISRSYKELVYLDLSKGG"
BASE COUNT         1220 a          483 c          729 g         1191 t
ORIGIN      
        1 gaggtcatac atttgattta gcagatgatt ggaaagagat tgattttgta gaggcaatta
       61 aagagaattt taatgtagat attgtaaatg ataatgagga gagcatgcta aaagctctta
      121 aagataaagg tttggaggta gaagaaggta tgaatagaaa tagaatgaca gatagtatgt
      181 ggaagtatgt aagaagtagt atttctggtc ctgcattttt agtaaatcat cctatgtttg
      241 tttctccttt agctaaatca aaagaggaga atcaaaacat aacagaaaga tttcaggtta
      301 ttattgcagg aagtgaactt ggaaatggat actctgaaat aaatgatcca caggatcagc
      361 ttatgagatt tttagaacag cagaaactta gagaaagcgg tgatgaagag gctcagatgc
      421 ttgatattga tttcgttgaa atgcttgaat atggtatgcc tcctgtaagt ggatatggtc
      481 agagtgaaag agtcttttgg tttctagaga atattactgc aagggaggga acactatttc
      541 cacagatgaa gcccgagctt gaacagtcta ctaaaaagat ttacaaggat atattgccta
      601 aggtaaagaa aaaataaatg gattccatta aggatatctt tacaaaactt aaagataatc
      661 agtcaaagaa aaaatcccag caggagttgt ttgatacaga cgaatactct aaaaagagag
      721 taatgttaaa tgatagacac aagtatatta gtaaagagta tcaatactat ggacttcatc
      781 ttgcaggaaa gttaggggat aagaaaagag caacaatgta tattaagtgg gctaaggaaa
      841 aaccaagagt aatattagaa aaagcatata gctttgcaat tgattatcct aatgcaaaag
      901 atagaagcag gatatttatg tggaaggtta aagagcttga agaacagatg aagggtgaga
      961 agaaggttga gagctaagaa ctttgtttac tacaccaagt actatttctg aagctttgtc
     1021 tccttcttct gcttgtacaa ctgctgcaga ggcatactga tgacctcctc cgcctaaagc
     1081 ttctgccata gttgctacct cttgatagtc tttactactt ctaaatgata ctttccattc
     1141 attttgtgct gttgaagaag gtttaatgat aaatccccaa tgtactcctt gtatgtacct
     1201 tataatatta tctcttacaa actcatatgc tccgtttatt cctatttgtg taattcctaa
     1261 actttcaata tcttttttat ttatatatgt atatgccata tctcctacaa tatgtatatt
     1321 ttcaatatat acttttattg gtgtaaggga acttgctgga aactttgcac ttttgtactc
     1381 aaactcctct atattaagac ggtatacact agtaagcttg gccatcattt cataagtgtg
     1441 aggtgtagta ttatcatatg caaatctgcc tgtatcatat actattccaa tttgtcctaa
     1501 tattgatact gtgggtgtaa ttttgtatct tttctgaaca ctcatacaga aatcaagaat
     1561 ctgttcagtt gtagaactca tctggtcatt aataactaca tctgcttgta ttccttttgt
     1621 tatatgatgg tctactactg ccacttttgc ttttgtaacc tgtattgtat ctccttttga
     1681 tacacacatg ccccatcctc cagcatccat aatgattaca agctcttcat tctctgtatt
     1741 aaactcttgt actatggtta tctcctccat taaaggtatt ttttgagatg ggttttgttt
     1801 ggttttaaat actacattct ttttaggata gaaggtgtta atatagtttt tgagtataag
     1861 ggagcttgca aatgcatcaa agtctggtcc tctatgtgta attatcaata tattgtttga
     1921 tttttctatc agttttttaa gttttttgta ttcttctttc atttctgcct acagtatatc
     1981 agattttttg ttgattacag tatgtgtaga gaatataata aagaaattaa gatataaata
     2041 ttgtttgtat gatagatatt aaaaggatag tggagaataa ggaagaagta gagaaagcat
     2101 tatctaaaag aatgaaagta gaggatatgc atttagatga gattattgat ttatataaca
     2161 aaaggaaaga agttttaaaa gagtatgaaa gtaaaagggc acagcagaac agctttaaca
     2221 gtaaaatggc aggaattgaa aaagggtctg aagagtttaa gaagcttgtt tctgatttaa
     2281 agacactttc acaaaatgta aaagagctgg aaagaaatgt tgatgaatta acccagaagc
     2341 ttacctctct tgttgaggtg cttcctaata ttcccgatga agatgttgtg gcaggggaga
     2401 aggaggctaa tcaggttatt aaagttgttg gtgagaaacc catatttaat tttcctatta
     2461 aagatcatgt tgagcttggg gagagtcttg ggttatttga tttagaaaga gctgttaaga
     2521 tatctggaaa taattttgta atgtataaag ggtggggggc aaggcttgaa tgggcacttc
     2581 ttaactattt tataaactct catctttctg atggatatga aatgatcctt cctcctcata
     2641 ttgttggaga gaggtctgga tatactgcgg gtcagcttcc taaatttaag gatgatgttt
     2701 attggcttaa agacttagat caattcttaa ttcctactgc tgaaactgct ttagataatc
     2761 tttttagaga tgagattcta aatgaaaaag accttcctaa aaagtacttc tcatatactc
     2821 catgctatag aaaagaggca ggaagctaca gggctaatga gaaagggttg ataagggttc
     2881 atcagtttaa taaagtggag atgtatcaat atacaacaca ggaaggaagt gacttagcat
     2941 ttgaagagct tttaggaaaa gcagaaaagc ttgtagcaga tttaggtctt cattacagaa
     3001 taagtaaatt agcagcagga gattgttctg caccagcagc aagaacatat gatgtggagg
     3061 tatggcttcc tgcagtttct caatattatg aggtcagctc tatatctaat gtaagggatt
     3121 atcaggcaag gagaggtaat atgagataca gaaaggaaga tggaactatt gagtatatgc
     3181 atacacttaa tgcttcaggc cttgctacta gccgtcttat ggtagcctta gttgaaacat
     3241 atcagcaaga agatggtagt atacttgttc ctgatgtttt aagaaagtat attggtgtag
     3301 ataaaattga aaagaagtag tatgagttac tttttagcat ttgtacctga caaagagagt
     3361 gtgaaagaga ttcaaaatgt tgttaaagaa gcaaattctc ttttttctga ttttggtatt
     3421 cctgtaagat gggttaagcc tgatacatat catattactc tttactacct tggagaaaat
     3481 tatcccatat ttaaccagat gcttttaaaa aagaaattat caaaaattaa gtttagggag
     3541 tttaaaatat catttcaaaa tattaaggtt ggtatatcaa gaagttataa agaactggta
     3601 tatttagatt taagtaaagg agg
//
LOCUS       DAOSAB010000046         3519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_17765_length_3519_cov_2.885104, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000046 DAOSAB010000000
VERSION     DAOSAB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17765_length_3519_cov_2.885104"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /gene="tuf"
                     /locus_tag="PK432_01350"
     CDS             <1..215
                     /gene="tuf"
                     /locus_tag="PK432_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276713.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOK59604.1"
                     /translation="TGYKPQFYVRTADVTGEVSLPEGTELVAPGDNVELTIKLVVPVV
                     LEKGQSFAIREGGATVGKGVVTEIIK"
     gene            279..590
                     /gene="rpsJ"
                     /locus_tag="PK432_01355"
     CDS             279..590
                     /gene="rpsJ"
                     /locus_tag="PK432_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOK59605.1"
                     /translation="MSETSSKIRVKLKGYDSLVLDKSSRSIIETAISTGAKVAGPVPL
                     PTRKRKIAVNRSPFIYKSSIEHFEINTHKRVIDIIDPTPKTIDALQHLQMPAGVGIEI
                     Q"
     gene            595..1182
                     /gene="rplC"
                     /locus_tag="PK432_01360"
     CDS             595..1182
                     /gene="rplC"
                     /locus_tag="PK432_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOK59606.1"
                     /translation="MKAILGIKKGMTRIFVDDRVVPVTLVDVTGCKVANIRKDGKVEL
                     GLGNKKKTTKAQLGQYKELGYVPMYKWVVESELPEGFKVGSELVGETFNEGDTVTVTG
                     ISKGKGFQGVVKRHGFKGGPRTHGQSDRLRAPGAIGARTTPGRVYKGKRMAGRMGHDT
                     VTIKNKRIVGIKENILLISGPLPGSNGDLVKIYTE"
     gene            1185..1790
                     /gene="rplD"
                     /locus_tag="PK432_01365"
     CDS             1185..1790
                     /gene="rplD"
                     /locus_tag="PK432_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOK59607.1"
                     /translation="MEEKVKKEKTEEIKLNPKVWDVPYNADLVAQVLYVLNSNERKGT
                     SSVKGRGDVSGGGKKPWKQKGTGRARQGSIRSPLWVGGGVAFGPNIKNWKRKINKKMS
                     KEAICIVLSERNRNDAVKVVDINSKGEIKSIREKMEKNILKSTLVISDIQNLKLAVRN
                     IKGVNIADPMKVNVKDVVKSKAVVIDKDSINKLENRLLNGK"
     gene            1780..2076
                     /gene="rplW"
                     /locus_tag="PK432_01370"
     CDS             1780..2076
                     /gene="rplW"
                     /locus_tag="PK432_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOK59608.1"
                     /translation="MENKEIMLKPVISEKSYALANEDNKYVFFVDRDVNKIEIKKAVE
                     KKYKVKVLSVNITVKPGKKINEWKTNRVFRKSDLKKAVVTLKKGDKIDEFLTVK"
     gene            2076..2924
                     /gene="rplB"
                     /locus_tag="PK432_01375"
     CDS             2076..2924
                     /gene="rplB"
                     /locus_tag="PK432_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOK59609.1"
                     /translation="MGVKEFKPTTSTLRGTKLEDRSMLEKDSGPKSLTVAKKQKSGRN
                     NQGRITVRHRGGGYKRRIRKIDFKRDKFGIEAKVLGLYFDPNRSAHLALLQYADGEKR
                     YIIAPRGIKVGSRVVSNDVTDILPGNAMRIKNIPSGTIVHCVESKVGMGATFGRSAGQ
                     AIVVQGIDPTGKYVQIKMPSGEIRLVPADAMATIGQVGNEEKINVKLGKAGRSRNLGK
                     RPVVRGMAMHAVEHPHGGGEGRGVVGRAKDIWGHRVGTRTRKNKRTDKLIIKRRRTKQ
                     RPFAKK"
     gene            2942..3247
                     /gene="rpsS"
                     /locus_tag="PK432_01380"
     CDS             2942..3247
                     /gene="rpsS"
                     /locus_tag="PK432_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOK59610.1"
                     /translation="MSRSLKKGVYVDEKLQKKVEQALQSGSRKPIKTWARASTVTPQM
                     VGLTFAVHNGKKHEEVLIEESMIGHRLGEFTQTRKFKGHAKKGKLAKVYGSSGRFEE"
     gene            3252..>3519
                     /gene="rplV"
                     /locus_tag="PK432_01385"
     CDS             3252..>3519
                     /gene="rplV"
                     /locus_tag="PK432_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOK59611.1"
                     /translation="MEEKKVKIKIKNVAQSPLKLRLVANLIRGKDVNEALDILQFTNK
                     KGSIFLKKAVLSGVASANEKYGVDKDGLKISHISIDGAPTYKRGR"
BASE COUNT         1341 a          393 c          850 g          935 t
ORIGIN      
        1 taacagggta taagcctcag ttctatgtta gaacagcaga tgttacaggt gaggtttcac
       61 tacctgaagg tactgaattg gttgctcctg gagataatgt tgagttgaca attaaattag
      121 ttgtaccagt tgtattagaa aaaggacaga gttttgcaat tagagaggga ggagctaccg
      181 taggaaaagg agtagtaaca gagattatta agtaatcttc ttgcagggag agtaaatctc
      241 cctgcaattt taagtttaat ttagctcttt aagatagaat gagtgagaca agttcaaaaa
      301 tcagagtaaa gttaaaaggg tatgattctt tagttttaga taagtcctca agaagtataa
      361 ttgagactgc gattagcaca ggtgctaagg tcgcaggccc tgtacctctt cctacaagga
      421 aaagaaagat tgcagttaat agatctccat tcatctataa gagttcaatt gaacattttg
      481 agattaatac tcataaaaga gtaattgata tcattgatcc tacacctaag acaatagatg
      541 ccctacagca tcttcagatg cctgctggtg ttggtattga aatacagtaa atatatgaaa
      601 gcaatattgg gaataaaaaa aggaatgaca aggatttttg tagatgacag ggttgtgcct
      661 gttactttgg ttgatgttac tggttgtaag gtagcaaata ttagaaaaga tggaaaagtt
      721 gagcttggtc ttgggaataa aaagaaaact acaaaagcac agcttggcca gtacaaagag
      781 cttggatatg tgccaatgta taaatgggta gttgaaagtg agcttccaga aggatttaaa
      841 gtaggaagtg aattagtagg agaaaccttt aatgaaggag atactgtaac tgtaacaggg
      901 atttctaagg gtaaagggtt tcaaggtgtt gtaaaaaggc atggttttaa aggaggtcct
      961 agaactcatg ggcaatctga tagattaaga gcaccaggtg caattggagc aagaactact
     1021 cctggaaggg tgtataaagg aaagagaatg gcaggaagaa tgggtcatga tactgtaaca
     1081 attaagaaca aaagaattgt aggcataaaa gagaatattc ttttaattag tggtccgctc
     1141 ccaggttcta atggtgattt agtaaaaata tatactgagt aaatatggaa gagaaagtaa
     1201 agaaagaaaa aactgaagaa attaaattaa atcctaaagt atgggatgta ccttacaatg
     1261 ctgatttagt tgcacaagtt ctttatgtac ttaacagcaa tgaaagaaaa gggacttctt
     1321 ctgttaaagg gagaggtgat gtttcaggag gaggaaagaa accatggaaa cagaagggca
     1381 caggaagagc aaggcaggga tctataaggt cacccctctg ggttggtgga ggtgtagcat
     1441 ttggtcctaa cattaagaac tggaaaagaa agattaacaa aaaaatgtca aaagaagcta
     1501 tatgtattgt actttcagag agaaatagaa atgatgcagt taaagtagta gatattaact
     1561 ctaaggggga aattaaaagt attagagaga aaatggaaaa gaatatattg aaaagcactc
     1621 ttgtaattag tgatatccaa aatttgaaat tggcagttag aaatattaaa ggagttaata
     1681 ttgccgatcc aatgaaagta aatgtaaaag atgttgtaaa gagtaaagca gtagttattg
     1741 ataaagattc tattaataaa ttagagaata ggcttttaaa tggaaaataa agaaattatg
     1801 ttaaaacctg tgatatcgga aaagagttat gctcttgcaa atgaggataa taaatatgtg
     1861 ttttttgtcg acagagatgt taataaaatt gagattaaga aagctgttga gaagaaatac
     1921 aaagtaaagg ttcttagtgt aaatattact gttaaaccag ggaaaaagat aaatgaatgg
     1981 aagactaaca gagtgtttag aaaaagtgat ttaaagaaag ccgttgttac tcttaaaaag
     2041 ggtgacaaga ttgatgaatt tttaactgtt aaataatggg agtaaaagaa tttaaaccaa
     2101 caacctctac attaagagga acaaaattag aagatagatc catgcttgaa aaagatagtg
     2161 ggccaaaatc gttaactgtt gctaagaaac agaagtctgg caggaataat caaggaagga
     2221 ttactgtaag gcatagaggt ggtggatata aaaggagaat aaggaagatt gattttaaga
     2281 gagataagtt tgggattgaa gctaaggtgt taggtttgta ttttgaccct aacagaagtg
     2341 cacatttagc acttttacag tatgctgatg gtgaaaaaag gtatataatt gctcctcgag
     2401 gaataaaggt aggtagcagg gtggtttcta atgatgttac agatatcctt ccaggtaatg
     2461 caatgagaat taagaatatt ccttcaggta ccattgtaca ttgtgttgag agtaaggttg
     2521 gaatgggagc tacctttgga aggtcagcag gtcaagcaat tgttgttcag ggtattgatc
     2581 ctactggcaa gtatgtacag ataaagatgc cgtcaggtga aattaggctt gttccagcag
     2641 atgctatggc aacaatagga caggtgggaa atgaagagaa gattaatgta aagcttggaa
     2701 aagcaggaag aagcaggaac ctaggtaaaa ggccagttgt tagaggaatg gcaatgcatg
     2761 cagtagagca tcctcatgga ggtggagaag gtcgtggagt tgttggtaga gccaaagata
     2821 tctggggtca tagagttggt acaagaacaa gaaagaacaa aagaactgat aaattaataa
     2881 ttaaaagaag aaggactaaa caaaggcctt ttgctaagaa ataatttaga agagtataaa
     2941 tatgtctagg tcactaaaga agggagtata tgtagatgaa aaattacaga agaaagtaga
     3001 gcaggcactg caaagtggta gtaggaagcc tattaaaaca tgggcaagag catctacagt
     3061 tacaccgcag atggtggggc ttacctttgc tgttcataat ggtaaaaagc atgaagaggt
     3121 tttaattgag gagtctatga taggacatag gcttggtgaa tttacacaga caagaaagtt
     3181 caaaggtcat gcaaagaagg gtaagttagc taaagtatat ggaagcagtg gtagatttga
     3241 agaataataa tatggaagaa aagaaagtaa aaataaaaat taaaaatgtt gcacaatctc
     3301 ctcttaaatt gaggttggtt gctaatttaa taagaggaaa agatgtgaat gaggccttag
     3361 atattttgca gtttacaaat aaaaagggaa gtatattctt aaagaaagct gttctttcag
     3421 gagtagcttc tgcaaatgaa aaatatggag ttgataaaga tggacttaag atatctcata
     3481 tatcaataga tggagcccca acatataaaa ggggaagat
//
LOCUS       DAOSAB010000047         3386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_18685_length_3386_cov_2.996397, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000047 DAOSAB010000000
VERSION     DAOSAB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18685_length_3386_cov_2.996397"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PK432_01390"
     CDS             complement(<1..615)
                     /locus_tag="PK432_01390"
                     /inference="COORDINATES: protein motif:HMM:NF020014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOK59612.1"
                     /translation="MKYVCSSCGSEFLKWSGRCSNCGQWGTLEEVDEEVSKENSKVSS
                     SKSPYKSIIEYRRSTDSKKGAKNRLGTGFKELDRVLGGGLIEGEVVLVSGEPGVGKST
                     LLLQVALNLSAKKKILYVCGEESPSQLISRLDRLSVDGGKPENIFVTNDVIVENIYNL
                     VSEEKFDLVIVDSIQSVTSITSKGYPGSISQVRASGSLLTRLSKV"
     gene            complement(629..3103)
                     /locus_tag="PK432_01395"
     CDS             complement(629..3103)
                     /locus_tag="PK432_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOK59613.1"
                     /translation="MERTKVKDTFKRMSKNLRISLRTSSIVSDHLKSDYIEPEHLLAG
                     ILLNENALATKIVSSMGLNIQDVVGRILGNLGMDITVNVDIPMEVNLSLETKDILRKA
                     YSWSQRFSHVYVGTEHIMLAILESKNFFTSSLNEIGLTQKRFEKYLFEYATYPLGILS
                     KPKITVSGREEGKLLDVIGRDLVELARAGKLDPLVGREKELSDIIKILSRRKKNNPII
                     VGDAGVGKTVLVEGLAQRIADGRVPSSLRDMRIISVDVASIMAGSRMRGDIEEKVLEM
                     VDEVVESNNIILFIDEIHNIFTSGIPGAPSEIGSILKPALLKEDFRCIGATTTEEYSA
                     YFENENALTRRFQPVYLKEPSIDESIQILKNLKPIFEAHHGVNISEEAVELAVTLSDR
                     YISDRYLPDKAIDLLDEACAAKRLSVEGEYENISSLTAKLRSIQNAKREYILQGKMDI
                     AERYKKSEDILVKRIKKLKNEKEKGRGNKDREVNAEDIRVVVSNWTGIPLNTLGSKEK
                     SALLKLDSKINKQIIGQKEAVESVVSAIKRARTGISDVSRPWASFLFLGPTGVGKTQL
                     AKVLAKELFGDEDRLIQIDMSEMMEMHSVSKLIGSPPGYVGYQEGGWLTEKVKRNPHS
                     VILFDEIEKAHPDVLNILLQILEYGHLTDGRGRRVNFKNTIVILTSNIGAEEISRDKV
                     LGFSSGGQQKSKSSSEQAYSAMKSDLMLELRRTLRPELLNRLDDIVIFRALNTRDARK
                     IVELLLDELNKRLSTQGISVSLSKSLMSYIVKEGFSEEYGARPLRRVLQDVVESTIAD
                     YLLKTESDGKKEIKLDIKGGKIFVLN"
     gene            complement(3160..>3386)
                     /locus_tag="PK432_01400"
     CDS             complement(3160..>3386)
                     /locus_tag="PK432_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOK59614.1"
                     /translation="GLIHISELSDKLVKDPGDIVKVGQKVKVKILSISSTERHLGLSL
                     KETSEVVGKKKMDEKELAEAVNAAIESEVK"
BASE COUNT         1042 a          801 c          392 g         1151 t
ORIGIN      
        1 aacttttgaa agcctcgtta aaagagagcc agaagctctc acctgactaa tactccccgg
       61 ataccctttt gatgttatag aggtaacact ttgtattgaa tctacaataa caagatcaaa
      121 cttttcttca gatacaagat tatatatatt ctcaacaatt acatcattag taacaaaaat
      181 attctctggc ttacctccat ctactgataa cctatctaat cttgatatta attgagaagg
      241 agactcctca ccacaaacat acaaaatctt cttctttgca gaaagattca atgcaacttg
      301 aagaagtaat gtagattttc caacaccagg ctcaccgctg acaagtacaa cctctccctc
      361 tattaaaccc cctccaagta ccctatccaa ctctttaaag cctgtaccta aacgattctt
      421 agcccccttt ttactatcgg tacttcttct atattctata atagacttat agggagattt
      481 agaactacta accttgctat tctccttgct aacttcctca tctacttctt ctagtgttcc
      541 ccactgacca caattactac atcttcctga ccattttaga aactcactcc cacaggagct
      601 acatacatat ttcatttgtt aactcttttt agtttagaac aaaaatttta ccacccttaa
      661 tatcaagctt gatctccttt ttcccatcgc tttcagtttt taaaagataa tcagctatag
      721 tactttcaac aacatcttgc aaaacacgcc ttaaaggtct agcaccatac tcctcgctaa
      781 aaccctcttt aacaatgtaa gacattaaag acttgcttaa tgatacagat ataccctgtg
      841 tacttaacct tttatttagt tcatcaagca aaagttcaac aatctttcta gcatctctag
      901 tgttcagagc tctaaatatt acaatatcat caagcctatt aagaagttca ggccttaaag
      961 tccttcttag ctcaagcata aggtcggatt tcattgcact atacgcctgc tcagaactac
     1021 ttttactctt ctgctgacct cctgacgaaa aaccaagcac tttatctcta ctaatctctt
     1081 cagctccaat atttgaagta agaataacta ttgtattttt aaaattaacc ctccttcccc
     1141 ttccatcagt aagatgccca tattcaagta tctgaagtaa aatattaagc acatcaggat
     1201 gagctttttc aatctcatca aaaagaatta cagaatgagg atttctttta accttttcag
     1261 tcagccatcc accctcttga tatccaacat acccaggagg agaacctatt aacttagaaa
     1321 cactatgcat ctccatcatc tcactcatat ctatttgtat aagcctatcc tcatctccaa
     1381 aaagctcctt tgcaagaacc ttcgctaatt gtgtttttcc tacacctgta ggtccaagaa
     1441 aaagaaatga tgcccacggc ctgcttacat cagaaatacc cgtccttgct ctctttattg
     1501 cagaaacaac actttcaaca gcctctttct gcccaataat ctgtttgttt atctttgaat
     1561 ctaactttaa aagtgcactc ttttctttac ttcctaatgt atttaatgga atacctgtcc
     1621 aatttgatac caccactctg atatcctcag cattcacctc cctatcttta tttcctcttc
     1681 ctttttcttt ctcatttttt agctttttta tccttttaac tagtatatcc tcgctcttct
     1741 tgtatctctc tgcaatgtcc atcttccctt gtagaatata ctctcttttc gcattttgaa
     1801 tgcttcttaa ctttgcagta agtgaagata tattttcata ttctccctca acgcttaatc
     1861 tctttgcggc acaagcctca tcaagaagat caatcgcttt atcaggcaaa tacctatcac
     1921 taatatatct atcagaaaga gtaacagcca gctcaacagc ttcctcactt atatttaccc
     1981 catgatgtgc ttcaaaaata ggtttaagat ttttaagaat ttgaatactt tcatctatac
     2041 ttggctcttt tagatataca ggctgaaacc ttcttgttaa agcattttca ttttcaaaat
     2101 atgcagagta ctcttcagtt gttgtagcac caatacatct aaaatcttct ttcaaaagag
     2161 caggttttaa gatagaacca atttctgaag gagcaccagg aatgccacta gtaaagatat
     2221 tatgtatctc atcaataaac aaaatgatgt tgttagattc aacaacctca tcaaccattt
     2281 ctaaaacctt ttcttcaata tctcctctca ttctgctgcc tgccataata cttgctacat
     2341 caacagaaat tatcctcata tctcttagtg atgaaggaac tcttccatca gcaatccttt
     2401 gcgccaaccc ttctactaaa acagtctttc ctacaccagc atctcctaca attattggat
     2461 tatttttctt tcttctacta agtattttta taatatccga caactccttt tctcttccta
     2521 caagcggatc cagcttacct gcccttgcaa gttcaactaa atctctccct attacatcta
     2581 acaacttccc ctcttcacgt ccagagacag taatctttgg tttagaaaga atcccaagag
     2641 gatatgttgc atattcaaaa agatattttt caaacctttt ttgtgttaaa ccaatctcat
     2701 taagacttga agtaaagaag tttttagact ctaaaattgc aagcattata tgttcagtac
     2761 ctacatatac atgagaaaat ctttgtgacc aactgtatgc ctttctaaga atatcttttg
     2821 tctctaatga aagattaacc tccattggaa tatccacatt aacagtaata tccatcccca
     2881 gattccctaa aattcttcct acaacatctt gaatattcag gcccatactg ctaacaatct
     2941 ttgtagcaag agcattctca tttaaaagaa tacccgctag aagatgttca ggttcaatat
     3001 aatcactttt taagtgatct gaaacaatag aggatgtacg cagtgatatg cgcaaattct
     3061 tcgacatcct tttaaatgta tcttttactt tcgttctttc catacttaat tataccaaaa
     3121 aataagggca gaaaaccgat atatgggata aaataatgac tatttaacct ctgattctat
     3181 agcagcattt acagcttcag ctaactcttt ctcatccatc ttctttttac ctacaacctc
     3241 agaagtttct ttcaagctca aacctaaatg cctttcagtt gaagaaatag aaagaatttt
     3301 aactttaacc ttttgtccta cttttactat atctccagga tcctttacta atttgtctga
     3361 aagctcagag atatgtatta atccat
//
LOCUS       DAOSAB010000048         3380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_18720_length_3380_cov_2.741955, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000048 DAOSAB010000000
VERSION     DAOSAB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18720_length_3380_cov_2.741955"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="PK432_01405"
     CDS             <1..1821
                     /locus_tag="PK432_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HOK59615.1"
                     /translation="LITPIIGIAIGGHFIQTQGDELKQSITELNFDEIEKTTESLSTN
                     ASRVEDSFNRLSWLFKLLGKNREYVDLFQVIQGTKYIAESGQNLATGLKPLGLYVKDF
                     TPSINFESQNENAQEYTSYLEAIKNNRHLLQEGVYKISLADSLISSADTRGYPSSLKN
                     TINEYKDLVSQIRTSVEPIEKISEFLPELLGLNQRRRYLILFQDNGEIRSTGGWISDY
                     AIVAIENGQIRELFVDDIYNAQALLKLKGKTYKTPTSMLKALGGVPFSFPLVNWDPNL
                     ENVLLYSEQFVYDLGKGNSLDGVITLDTVFIQRLLDKWGGIEVAGESELINSENFYPR
                     VFKEPTTGLVSDPRTSVFLADFFNSVITKIFSNKITDNKDVYEVVKDSIDEKHILFSF
                     KNASAQSYLNENGWVSNLEQKFAVAPINIDWNWGGNKANLYIKKNHTLNIQVKDEKTI
                     DYTYQITIENDSTKEEFPQGEYRNYIRIYVPYNAEITALRGLTNNSYDMYIENGYKVI
                     AGWFNIPIQQTLTLELRYRLNKTANNTNFPLLKTDTNYQMDIYIFKQPGSRKDSYSLS
                     LSYPSEWIVENSEGLTRIENNVNRRFELSSDQHFYISWQR"
     gene            complement(1823..2152)
                     /locus_tag="PK432_01410"
     CDS             complement(1823..2152)
                     /locus_tag="PK432_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59616.1"
                     /translation="MAKDLNDKKQFFIDTIAKFCDKCGHPYSKEDLEIIQDTDISSII
                     HFSCPNCKSNHIATYFKPIGISNRMPINTDLDVEEISKFASMRETSVEEILDLYLYLK
                     GKEKVRI"
     gene            2179..3264
                     /gene="rpoD"
                     /locus_tag="PK432_01415"
     CDS             2179..3264
                     /gene="rpoD"
                     /locus_tag="PK432_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOK59617.1"
                     /translation="MNKSNKSKYIEQLVKKGKQQGFLSQEDILEIFPTIEEDIVLLDD
                     IFKELQENEIEVLEPEENISPDSENLTLEKKIRILKTIQSTLSTDAIRSYLYEIGKIP
                     LLTADEEVILAKRIEKGDKEAAQLLITANLRLVVSIAKKYSKSGLELLDLIQEGNIGL
                     MRAVEKFDYHKGFKFSTYATWWIRQAITRAIADQARTIRVPVHMIETINKFNKVSTTL
                     ATRLGRQPTDEEIAKEMDIELDKIAEIRKIKQNPSSLATPIGDEKDSKLQDILPDEWS
                     QSPEDYASSEYLKNQLREILDSLQDRERKVLSLRFGLTDGVSRTLEEVGREFGVTRER
                     IRQIEAKALKKLKEKSSDKKLDDYINR"
BASE COUNT         1328 a          509 c          572 g          971 t
ORIGIN      
        1 ttaataactc caataatagg gattgcaata ggagggcact ttatacaaac acaaggagat
       61 gaacttaaac aatcaattac agagcttaat tttgatgaaa tagagaaaac aacagaatct
      121 ctttctacca atgcatcaag agttgaagat agttttaata gactttcatg gctatttaaa
      181 cttttaggaa aaaatagaga atatgtagat ctcttccagg taatccaagg aacaaagtat
      241 attgctgaaa gcggacaaaa ccttgctact ggccttaagc cattagggct gtatgtaaaa
      301 gacttcacac catctattaa ttttgagtcc caaaatgaaa atgcacaaga gtatacttca
      361 tatcttgaag caattaaaaa taacaggcac ttacttcaag aaggagttta caaaatatct
      421 ctagcagaca gccttatttc ttctgcagat acaagaggat atccctcatc attaaaaaat
      481 acaataaacg aatacaaaga tttagtttct caaattagaa catctgtaga accaattgaa
      541 aaaatatctg agtttctacc agaactactt ggattaaacc aaaggagaag gtatctcata
      601 ctatttcaag ataacggaga gatacgaagt accggtggat ggatatcaga ttatgcaata
      661 gtagcaatag aaaatggcca gataagggaa ctgtttgtag atgatatcta taatgctcaa
      721 gcattattaa aattaaaagg aaagacatac aaaactccaa cctctatgct taaagcctta
      781 ggtggtgtcc ctttctcttt cccattggtt aactgggatc ctaatctaga gaatgttctt
      841 ctttactctg agcaatttgt ctatgattta ggaaaaggaa actctctaga tggagttatt
      901 acattagata cagtttttat tcaaaggctc cttgataaat ggggcggaat agaggtagct
      961 ggagaaagtg aattaataaa ttcagagaac ttctacccaa gagtttttaa ggaacctaca
     1021 actggtttag tttctgatcc aagaacatct gttttcttag cagacttctt taattctgta
     1081 attacaaaga tattttcaaa caagataaca gataataaag atgtatatga ggttgtaaaa
     1141 gactctattg atgaaaaaca tattcttttc tcatttaaaa atgcttcagc tcaatcatat
     1201 ttaaatgaaa atggctgggt gagtaattta gaacagaagt ttgcagtagc acctataaat
     1261 attgattgga actggggtgg gaataaggcc aatctatata taaagaagaa tcatacatta
     1321 aatattcaag ttaaagatga aaaaactatt gattatacat atcaaattac aatagaaaat
     1381 gactctacta aagaagagtt tccgcaaggg gagtatagaa actatataag gatatatgta
     1441 ccatacaatg cagaaataac agcacttcgt ggattaacaa ataatagcta tgacatgtat
     1501 atagaaaatg ggtataaagt aattgcaggt tggtttaata tccctattca acaaacttta
     1561 acattagaac ttagatacag attaaataaa acagctaata atacaaactt cccattgctt
     1621 aaaacagata caaactatca gatggatata tacatattta aacagcctgg gagcagaaaa
     1681 gattcataca gcctctcttt aagctacccg tcagaatgga ttgttgaaaa cagtgaagga
     1741 ttaacaagaa tagaaaataa tgtaaataga agatttgagc tatcatcaga ccagcacttc
     1801 tatatatcat ggcaaagata gtctagattc taactttctc tttccctttt aaatacaggt
     1861 ataaatctag tatctcttca acagatgttt ctctcataga tgcaaatttg ctaatctctt
     1921 caacatctaa atccgtgttt ataggcattc tattgcttat accaataggt ttaaagtatg
     1981 tagcaatgtg atttgattta caatttgggc atgagaagtg tataatactt gagatgtctg
     2041 tatcttgtat tatttctaaa tcctctttag agtaaggatg accacattta tcacaaaatt
     2101 tagctatcgt atcaataaaa aattgttttt tatcatttaa gtctttggcc atatattaaa
     2161 ttataccata acagtaaaat gaacaaatca aataaatcaa aatatataga acaacttgtt
     2221 aaaaaaggaa aacaacaggg ctttctctca caggaagata ttttagagat attccctaca
     2281 atagaggaag atattgtact acttgatgat atttttaaag aactacaaga aaatgaaata
     2341 gaggtacttg aacctgaaga aaatatatct ccagatagtg aaaatctaac tcttgagaaa
     2401 aaaataagaa tacttaaaac aatacaatca acactatcta cagatgcaat aaggtcatat
     2461 ctttatgaaa taggaaaaat tccacttctt accgcagatg aagaggttat acttgctaaa
     2521 agaattgaaa aaggtgataa agaggccgca cagcttttaa ttactgctaa cttaagattg
     2581 gttgtaagca ttgctaaaaa gtacagtaaa agcggattag aactcttaga tctaattcaa
     2641 gagggaaata tcggccttat gagagcagtt gagaagtttg actatcacaa aggttttaag
     2701 ttttctacct atgctacatg gtggataaga caggcaataa caagagcaat tgctgatcaa
     2761 gcaagaacta tacgtgttcc tgtacatatg attgaaacta taaacaaatt caataaagta
     2821 agtacaacat tagctactag acttggaaga cagccaacag acgaggaaat tgcaaaagaa
     2881 atggatatag agcttgataa aatagcagaa ataaggaaga taaaacaaaa tccttcttct
     2941 cttgcaaccc caattggaga tgaaaaagat agtaaattgc aggatattct tcctgatgaa
     3001 tggtcacaaa gcccggagga ttatgcaagt tctgaatatc taaaaaatca acttagagaa
     3061 attcttgact ccctgcagga tagagaaaga aaagtccttt cattaagatt tggactaaca
     3121 gatggtgtaa gcagaacatt agaagaagta ggcagagagt ttggagtaac aagagaaaga
     3181 ataagacaaa ttgaagctaa ggcattaaag aaacttaaag aaaaatcatc tgacaaaaaa
     3241 cttgatgatt atataaatcg ttaattggct gactcctctg ttttaaccat atcaaaaaag
     3301 gaagaaggtg gaatagcata tgtatcatca actctcttag atacatcatt taacacctca
     3361 tcatcaaaag aagaatgtaa
//
LOCUS       DAOSAB010000049         3369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_18789_length_3369_cov_3.136693, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000049 DAOSAB010000000
VERSION     DAOSAB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18789_length_3369_cov_3.136693"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..299)
                     /locus_tag="PK432_01420"
     tRNA            complement(226..299)
                     /locus_tag="PK432_01420"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(263..265),aa:Val,seq:cac)
     gene            complement(277..507)
                     /locus_tag="PK432_01425"
     CDS             complement(277..507)
                     /locus_tag="PK432_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59618.1"
                     /translation="MKYVIAGAVSIVLAVIAGIFGNVLTGVLIFVLVFGGYIMLNIVN
                     DLKPSEAKQVNITNVDQKNHSDKATRTISSAG"
     gene            complement(577..650)
                     /locus_tag="PK432_01430"
     tRNA            complement(577..650)
                     /locus_tag="PK432_01430"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(614..616),aa:Val,seq:tac)
     gene            complement(696..1043)
                     /locus_tag="PK432_01435"
     CDS             complement(696..1043)
                     /locus_tag="PK432_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59619.1"
                     /translation="MGRERKYMPFLFFFIFVIFILGFSKMMESIKPATRVAALKGSMC
                     GDMLISQAIIIAQGSECMEVGSLNVSEYECNEEIKRISFPMNMKDESSEKSAVCYVFT
                     NSGNAYLQLKNSK"
     gene            complement(1075..1161)
                     /locus_tag="PK432_01440"
     tRNA            complement(1075..1161)
                     /locus_tag="PK432_01440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1125..1127),aa:Leu,seq:gag)
     gene            1249..2517
                     /gene="eno"
                     /locus_tag="PK432_01445"
     CDS             1249..2517
                     /gene="eno"
                     /locus_tag="PK432_01445"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOK59620.1"
                     /translation="MKIKDINAIEILDSRGVPTVSVEIELLSGDKARGEAPSGSSTGS
                     NEALELRDNDPKRFNGKGVLTAVNNVNTIIRDAILDKEFESQKQFDDFLISLDGTENK
                     SKLGANAILSCSIAFCKAAALRIGLEPFEYIGMIYWDKEYSKDKFHMPRPQILMMEGG
                     KHGNWATDIQEYMIVPKPNSFETFSEILKASSEIFYCIENILDEKGYSTGVGYEGAFA
                     PREIKSNKEALDIIMDGIQKAGYSEKFDMALDIASSELFNNDTQRYELKRENKSINAQ
                     EWITLQSEWYSQYPVFSIEDPLDENSWEQWSTFTKSFGDKYQIVGDDLLVTNTKLIQK
                     GINEKAMNAVLIKLNQIGTVSETLDAIRMSVNNGMQAIVSHRSGETNDSFIADMVVGT
                     AAQQSKFGAVTRGERVVKYNRLLEIEEYLL"
     gene            complement(2512..>3369)
                     /gene="tsaD"
                     /locus_tag="PK432_01450"
     CDS             complement(2512..>3369)
                     /gene="tsaD"
                     /locus_tag="PK432_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOK59621.1"
                     /translation="GMIHNVDYIAVTIGPGLAIDLDVGIKYAKELAVRYNKPFVPVNH
                     MEGHMFSSLLLNSKGKGLVNDDELLNAFPALALLMSGKHTEIVYIKSIGKYKKLGQTL
                     DDGAGEAFDKVGRLLNLGYPGGPVVSEFAKRGRRGVIEFSTPMKNSNDFNFSYSGIKT
                     AALYKAKELREKGIPEKEWIYDFCRGFVDAIIDSVVIKLKKVIEKKSDVKSIFVGGGV
                     FNNEEILRAVGRVSKEYGLNYYYPEIEYRSDNAAMIALSGYFNVLQKKVLTEKEDILV
                     VDRDPRLSL"
BASE COUNT         1240 a          638 c          493 g          998 t
ORIGIN      
        1 cacaataagt aggcacttcc ctctccgtgt tattcttact tttagttacc tgtgtatata
       61 caatccatgt aattaaaaga aaagcaaata tcccaaacat aacatatatc ttcttcaggt
      121 ttgtactctc attattactt cctgaagaca tgctggtatt ttttgtatcc actatgtata
      181 aagatacaat ttaaatatat cttagtgtac cacttataaa aaatatggac aatagtagat
      241 tcgaactact gacccctaca atgtgaatgt agtgctctaa ccagctgagc taattgtcct
      301 tgtagcttta tcgctgtgat tcttttgatc tacattagta atatttacct gcttagcttc
      361 acttggctta aggtcattaa caatattaag cataatatac cccccaaata ccaaaacaaa
      421 tattaaaacc ccagttaaaa cattaccaaa tatccctgct attactgcaa gtacaatact
      481 aacagcccca gctataacat acttcattaa atctatcatt taaatatgtt attcaaatta
      541 tttttacatt ataacctaaa aaacggataa caaaaatggg cgatatagga ttcgaaccta
      601 tgaccccttc ggtgtaaacg aagtgctctg accagctgag ctaatcgccc ataaacaaac
      661 tgtattctat caaaaaactc ttcctttatc caattttact tactattttt aagttggaga
      721 taagcatttc cagagttagt aaatacataa caaacagcac tcttctcact actctcatct
      781 ttcatattca taggaaaaga tattctctta atctcttcat tacattcata ttcagaaaca
      841 tttaaactac ccacctccat acactcgcta ccctgagcta taattatcgc ttgagatata
      901 agcatatcac cacacataga gccttttaag gcagcaaccc tagtagcagg tttaatactc
      961 tccatcatct tagaaaaacc aagaataaag ataacaaaga tgaagaagaa aagaaaaggc
     1021 atatactttc tctctctacc cattgacatg gcagtaatgt atacaaagta aaaatgccga
     1081 ggacaggatt tgaacctgca caggagtata agtcccacta ccacctcaag gtagcgtgtc
     1141 tgccaatttc accacctcgg cctgtacata ttctatcata taccatatta cttgctcaat
     1201 tccctacttt atgacaaaat caaatagata atttatctaa ggcgtaatat gaaaataaaa
     1261 gatataaatg caattgaaat actagattca agaggagtac ctacagtttc tgttgagata
     1321 gaattgctaa gcggagataa ggcaagagga gaagcaccat ctggatcatc tactggaagt
     1381 aatgaagcct tagaattaag agataatgat ccaaagagat ttaatggcaa aggagtacta
     1441 actgctgtta ataacgtaaa tacaataata agggatgcta tacttgataa agagtttgaa
     1501 agtcagaaac agtttgatga ttttttaata tcacttgatg gtacagaaaa caaatcaaaa
     1561 cttggcgcta atgcaattct gtcatgttca atagctttct gcaaagcagc tgcacttaga
     1621 ataggtcttg aaccttttga atatataggg atgatatact gggataaaga gtactcaaag
     1681 gataaatttc acatgcctag acctcagata ttaatgatgg aaggaggaaa acacggcaac
     1741 tgggcaacag atatccaaga gtacatgatt gttccgaaac ctaactcttt tgaaaccttc
     1801 tctgaaatac ttaaagcttc tagtgaaata ttttactgta ttgaaaatat tttagatgaa
     1861 aaaggatatt caacaggtgt aggatatgaa ggagcatttg caccaagaga gattaaatca
     1921 aacaaagagg cattagatat tattatggat ggtatacaaa aagcaggata ttcagaaaaa
     1981 tttgatatgg cactagacat tgcttcttca gagctattta ataatgatac tcaaagatat
     2041 gaacttaaaa gagaaaacaa atcaattaat gcacaagaat ggataacact ccaaagtgag
     2101 tggtattcac aatatcctgt attttcaata gaagacccat tagatgagaa ctcttgggag
     2161 cagtggagta catttactaa atcatttgga gataaatatc aaattgtagg agatgattta
     2221 cttgttacaa acacaaagct aatacaaaaa ggtataaatg agaaagcaat gaacgcagta
     2281 cttattaaac ttaatcagat aggtactgta tctgaaactt tagatgcaat aagaatgagt
     2341 gtaaataatg gaatgcaggc aatagtatca cacagaagtg gggaaactaa tgattcattt
     2401 attgcagata tggttgtagg aacagctgca cagcaatcta aatttggtgc agtaacaagg
     2461 ggagaaagag ttgtaaaata caacagactt cttgaaattg aagaatacct tttataatga
     2521 taatctagga tccctatcaa caaccaatat atcttccttt tcagttaaga ctttcttctg
     2581 aagtacatta aaatatccac ttaaagcaat catagcagca ttatcgctcc tatattcaat
     2641 ctcaggatag taataattaa gaccatactc tttagatact cttcctacag cccttaaaat
     2701 ctcctcgtta ttaaaaaccc ctcctccaac aaatatgctc tttacatcag atttcttttc
     2761 aataactttt ttcaatttaa taacaacaga atcaataata gcatcaacaa aacccctgca
     2821 gaaatcataa atccattctt tctcaggaat acccttttct ctaagctcct ttgccttata
     2881 caaagctgca gtcttaatac cagaatagct aaaattaaaa tcattagaat ttttcatagg
     2941 agtagaaaat tcaataaccc ctcttctacc cctctttgca aattcagaaa caacaggacc
     3001 accaggataa cctaaattaa gaagtcttcc tactttatca aaagcctctc cagcaccatc
     3061 atctaatgtt tgtcctaact tcttgtattt accaatactc tttatatata caatttcagt
     3121 atgcttacca gacataagaa gagcaagagc aggaaaagca ttaagaagct catcatcatt
     3181 taccaaaccc tttccttttg aattaagaag caaagatgaa aacatatgcc cttccatatg
     3241 attaacagga acaaatggtt tattgtatct aactgctaac tcctttgcat atttaatgcc
     3301 tacgtctaaa tctatagcaa gaccaggacc tatggttact gcaatataat caacattatg
     3361 aatcatacc
//
LOCUS       DAOSAB010000050         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_19030_length_3337_cov_3.163010, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000050 DAOSAB010000000
VERSION     DAOSAB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19030_length_3337_cov_3.163010"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PK432_01455"
     CDS             complement(<1..991)
                     /locus_tag="PK432_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59622.1"
                     /translation="MPISASKYIKNSESKKSYISMFNYKSLPRGGNGSIFDVYGFFSA
                     FSDIDIPGEQILKFAWDGLVDGFEYSKTVSINESLKSGLSEAANRVKQLIMNDKNIGE
                     HGVNVNFTVFVSTDEGMYIGVFGDSDIYIYKQGKLVDISEMLKSKGAKTAAVAMDDED
                     LIFASSQGYLKDNLLKLISSKSKNEIKSVLEDLGREIGDDMGIVIFSKERVEEKPKKE
                     ESVQEEKVIKPKRRLQPNLKQEPLDTDYIPVSKTSKKVFKPTVHEQDLKEVFSKVSKK
                     LEPAKNTIVKVSSASFQVVKNASGKIVSGVKNIGLSTKSKISNKLGRERWFKKV"
     gene            complement(997..1257)
                     /locus_tag="PK432_01460"
     CDS             complement(997..1257)
                     /locus_tag="PK432_01460"
                     /inference="COORDINATES: protein motif:HMM:NF040135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59623.1"
                     /translation="MENSILDKVLEFDFGQMGSLVILLKIPLIIILAVSIFYSFMLIL
                     KIRILHDTIDLEGSDKAKVLVYANLVVSLVVGILGIIIIMLG"
     gene            complement(1257..1889)
                     /locus_tag="PK432_01465"
     CDS             complement(1257..1889)
                     /locus_tag="PK432_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOK59624.1"
                     /translation="MKKGKSNIPKKFQNADSSFIDRLVKEEKDGACTGFKVFPKKLNF
                     YGKDPKEEVVLLTRLHWLAYLPDLLLVLLFLLLPILLSIISSVFNSSISPALYTGLFI
                     LGVLIALNVLVTAILKWFYSVIVITDRRFVVVSLKSGFYHSYSEAQLKNIEDVTHTMA
                     GFWGNIFDIGNLDVDTAGHEIDFRLKMVPKPRELQDIINDLVEMKKKGKI"
     gene            complement(1932..>3337)
                     /locus_tag="PK432_01470"
     CDS             complement(1932..>3337)
                     /locus_tag="PK432_01470"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOK59625.1"
                     /translation="QVIRSLFIKKNTITTLREEIEKITGEFQSLVDIKASLEKEKEEL
                     DKQKRDLADSYALIQKEKNRVQAELNKQIAEKGKIARTINGLTMELSDLQYQLLITRQ
                     GGTYVDPNAVPASGERITTLRGFMDSAPAGSFGVFSIGAYTHRNGMSQWGAKARANAG
                     QTYDQILAAYYPGKVLRTGSVVINGVQQNIMTNISTTTYGTLNFEEDYLLRLNEVPES
                     WPMEVLKAQAIAARTYAINYTSNGAKSICTSESCQVVGKSKKTGAWKQAVESTRGMVL
                     TDSSGKPFSTQYAAVHGGWSNTSGWDTTDKTGNGDWMSRAWDSISGVSWFYKIWYRGE
                     PPKNWYSDTAPNCGRNPWMTQEEMADIINIYQIWKASGEKGDSRIYPVVDACHSNIGQ
                     YTYAEARAKAAKPVTSISSVAVSSSNGTTNTVTFHTNAGIFVISGKDFKTVFNLRAPG
                     RLRIPQRDFVHINIHMK"
BASE COUNT         1046 a          701 c          421 g         1169 t
ORIGIN      
        1 atactttttt aaaccatctc tcccttccta atttgtttga tatcttactc tttgtactta
       61 aaccaatatt cttaacacca ctaactatct tcccagaggc attctttaca acctgaaaag
      121 atgcagaaga tactttgact atagtattct ttgcaggttc aagtttcttt gatacttttg
      181 aaaagacctc ttttaagtcc tgttcatgta cagtaggttt aaataccttt ttgcttgtct
      241 tagaaactgg tatatagtcg gtatctaagg gttcttgttt cagattaggt tgtaatctcc
      301 tcttaggttt aataactttt tcttcctgca cactctcttc ttttttaggc ttttcttcaa
      361 ccctttcttt tgagaaaata acaataccca tatcatcccc tatttctctc cccaaatctt
      421 ctaatacaga tttaatctca tttttactct tagaagaaat aagcttaagg aggttgtctt
      481 tcaaatatcc ttgagatgat gcaaatatta aatcttcatc atccatagca acagctgctg
      541 tttttgcacc tttgctttta agcatctctg atatatctac cagttttccc tgcttgtata
      601 tatagatatc actatctcca aagacaccta tatacatacc ttcatcagta gatacaaaaa
      661 cagtaaaatt aacattaaca ccatgttccc ctatattttt atcgttcatg ataagctgct
      721 ttaccctatt tgcagcttca ctaagtcctg atttaagtga ttcatttata gatacagttt
      781 tagaatattc aaagccgtct acaagcccat cccatgcaaa ctttaatatc tgctcccctg
      841 gtatatctat atcactaaat gcagaaaaaa aaccatatac atcaaaaata gaaccatttc
      901 ctccgcgagg taggctttta taattaaaca ttgatatgta tgactttttt gattctgaat
      961 tttttatata tttcgatgca ctaataggca tatatattat cctaacataa ttattattat
     1021 ccctaatatc cctaccacaa gagaaacaac aagatttgca tatacaagta ccttcgcttt
     1081 atcacttcct tctaaatcaa tagtatcatg cagtatccta attttaagaa taagcataaa
     1141 agaataaaag atagataccg caagtataat aatcagcgga attttaagaa gtataacaag
     1201 acttcccatt tgtccaaaat caaattctaa cactttgtct aaaatactgt tttccattat
     1261 atttttcctt tctttttcat ttcaaccaaa tcattaataa tatcctgcag ttctcttggc
     1321 ttaggtacca tttttaatct aaaatctatt tcatgtcctg cagtatcaac atctaaattc
     1381 ccaatatcaa aaatatttcc ccaaaaacct gccatagtat gtgttacatc ttcaatgttc
     1441 tttaattggg cttcagagta agaatgataa aaaccacttt taagactaac aacaacaaat
     1501 cttctatctg taataactat aactgagtaa aaccatttta gaatagcagt aacaaggaca
     1561 tttaaagcaa ttaacactcc aagaataaat aaacctgtat aaagtgcagg agatattgaa
     1621 gaattaaata cagaagatat tattgaaaga agtatgggta atagtaaaaa taataataca
     1681 agaagtaaat caggaagata tgcaagccaa tgtagccttg taagaagtac aacctcttcc
     1741 ttagggtcct taccataaaa atttaatttc ttaggaaata ccttaaaccc agtacaagca
     1801 ccatctttct cttctttaac taacctatca atgaaagaac tatcagcatt ttgaaacttt
     1861 ttaggaatgt tgctctttcc ttttttcata tacacaagaa tatcacaaaa gtatatgaat
     1921 taatagtact tttatttcat gtgtatatta atatgaacaa aatctctttg tggaattcta
     1981 agccttcctg gagcacgaag gttaaaaact gtcttaaaat ctttacctga gataacaaat
     2041 attcctgcat ttgtatgaaa agttactgta tttgtagtac cattgctact tgaaactgcc
     2101 acagaagata tcgaggtaac aggcttagct gcttttgctc ttgcctctgc ataggtatac
     2161 tgtcctatat tagaatgaca agcatctaca acagggtata tcctagaatc acccttctct
     2221 ccagaagctt tccaaatctg atatatattt ataatatctg ccatctcctc ttgtgtcatc
     2281 catggatttc ttccacagtt aggggcagta tcagaatacc aattcttagg aggttcacct
     2341 ctataccata ttttgtaaaa ccaagatact ccagagatac tatcccatgc cctgctcatc
     2401 cagtccccat ttccagtctt gtctgttgta tcccagccag aagtattact ccacccacca
     2461 tgaacagcag catactgcgt tgaaaatggt ttaccagaag aatcagttaa taccatccct
     2521 cttgtagact caacagcctg cttccatgct cctgtttttt tacttttccc aactacttga
     2581 caactttcag atgtacaaat agattttgca ccattactag tatagttaat agcatatgtt
     2641 cttgcagcta tagcttgtgc tttcaatacc tccataggcc aagattctgg tacctcattt
     2701 aatcttaaaa gataatcttc ttcaaagttt aatgtcccat atgttgtagt agaaatattt
     2761 gtcataatat tctgttgcac cccatttata actacagaac ctgttctcaa aacctttcct
     2821 ggataatatg cagcaagtat ctggtcatat gtctgccctg cattagctct agctttagcc
     2881 ccccactgac tcattccatt tctgtgtgta tatgcaccaa ttgagaacac tccaaaagaa
     2941 cctgcaggag cactatccat aaacccccgc aatgttgtaa tcctttcacc agaagcagga
     3001 acagcattag gatctacata tgttccccct tgtcttgtaa taagcaattg atattgaagg
     3061 tcagatagct ccattgtaag accatttatt gttctagcta tcttcccttt ctctgcaatc
     3121 tgtttattta attctgcttg tactctattc ttttcctttt gtattaatgc atatgaatca
     3181 gctaaatccc ttttttgttt atctagctcc tctttttcct tttccaaact agctttaata
     3241 tcaacaagac tttggaactc ccctgtaatc ttttctattt cttctcttag tgtagttata
     3301 gtgtttttct taataaacaa actccttata acctgat
//
LOCUS       DAOSAB010000051         3335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_19041_length_3335_cov_3.560671, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000051 DAOSAB010000000
VERSION     DAOSAB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19041_length_3335_cov_3.560671"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /gene="nifS"
                     /locus_tag="PK432_01475"
     CDS             <1..1079
                     /gene="nifS"
                     /locus_tag="PK432_01475"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOK59626.1"
                     /translation="DKYGNPSSIYSLAQESASAINEARKKVANALNAQENEIYFTSGG
                     TESDNWAIKGIADANKEKGGHIITSSIEHHAVLHTCQYLEKHGFEVTYLPVESNGIIN
                     LEELKKAIKDDTILISIMFANNEIGSIQPIAEIGKIAKEKGIYFHTDAVQAVGHIPID
                     VNELNIDLLSLSAHKFNGPKGVGALYIREGTKISNLLHGGAQERKLRAGTENVAGIVG
                     LGKAIELAVKDMDKHNKYITELRDNLINGLLKIPDTILNGDPKLRLPGNVNVCFKYIE
                     GESILLLLDQIGVCASSGSACTSASLKPSHVLLAIGLPAEIAHGSLRLTLSYENTQEE
                     VDYVLNQLPSIVEKLRALSPLIKK"
     gene            1086..1466
                     /gene="nifU"
                     /locus_tag="PK432_01480"
     CDS             1086..1466
                     /gene="nifU"
                     /locus_tag="PK432_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOK59627.1"
                     /translation="MEQYSKKVMDHFLNPRNVGEIKDADGIGEVGNPVCGDIMRMYIK
                     VKDNIITDIKFLTYGCGAAVATSSMVTEMVKGKTLEEALEISNKHVAEALDGLPPIKM
                     HCSNLAEEGIKAAIDDYKKKHDKK"
     gene            1453..2505
                     /gene="mnmA"
                     /locus_tag="PK432_01485"
     CDS             1453..2505
                     /gene="mnmA"
                     /locus_tag="PK432_01485"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOK59628.1"
                     /translation="MTKSNPTVLLGMSGGVDSSTTAYFLKKQGFNVTGITLSMFNEND
                     SDFKQNVKDAKETCKLLGIPHLVFNVEKDFKQYVINYFISEYEAGRTPNPCVICNRYV
                     KFEGLLTKADELGIEYISTGHYASVEKKDGKYLLKRGIDDKKDQSYFLYNLTQEQLSR
                     SIFPLGQYTKEQIREIARKNKLPASHKSESQEICFIKDNNYKKYIDENKTKNIPKGEF
                     VDTKGNIIGHHTGIQNYTIGQRRNLGISTGKPMYVLSIDGENNRIVLGTNNELFLTQL
                     VAKDLNWIMIDKLEKEMKVKAKVRYGAKPADARISPSQDNRVKVIFTTAQRAITKGQA
                     VVFYDNQYVVGGGIIS"
     gene            2574..>3335
                     /locus_tag="PK432_01490"
     CDS             2574..>3335
                     /locus_tag="PK432_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59629.1"
                     /translation="MSRRNKKKREIKKQIQSLRAVSLFLTLVITVLSTSIFLVSKNLQ
                     TDAQVKGESTFSINNKVNTSSYTNYFSDILGVEFIYDNTLFTISENSNYITLSTLNGS
                     LPFKAGTIRKVKDKDIKDIYPDSKYLETINDNGVGRTLFSYSVPSIIKEENEETEYLT
                     VIHKYFPNNITIYMQIWGYNFKDDLRTTRVLTELMESLTLATQKSKTSGVLSATSSIV
                     NQAQILGQASTVRIYSQKCNNVKFSDSLKPLSVGGN"
BASE COUNT         1338 a          470 c          607 g          920 t
ORIGIN      
        1 cggacaagta tggaaatccg tcaagtattt actcccttgc acaagaaagt gcctctgcaa
       61 taaatgaagc taggaaaaaa gtagcaaatg ccttaaatgc acaagaaaat gaaatatatt
      121 ttacaagtgg tggaacagaa tcagacaact gggctattaa gggtattgca gatgcgaata
      181 aagaaaaggg aggacatata ataacaagca gtattgaaca tcatgctgta cttcatacat
      241 gccagtatct tgaaaagcat ggttttgagg ttacatatct gcctgttgaa agtaatggaa
      301 ttattaattt agaagagtta aagaaggcaa taaaggacga cacaatatta atttcaataa
      361 tgtttgcaaa taatgagata ggtagtattc agccaatagc agaaatagga aagattgcaa
      421 aagaaaaagg aatatacttt catactgatg cagtacaagc tgtaggacac atccctattg
      481 atgttaatga attaaatata gatttgttat cactatcagc tcacaagttt aacggaccca
      541 aaggagtagg ggcattgtat ataagggaag gaacaaaaat atcaaatctt ttacatggag
      601 gagcacagga gagaaaactt agggctggaa cagagaatgt tgcaggtatt gtggggttag
      661 gaaaggcaat agagctagca gtaaaagata tggataaaca taataagtat attacagaat
      721 taagagataa tttaatcaat ggtttactta aaatacctga tactatatta aatggagatc
      781 ctaaattaag acttcctgga aatgtaaatg tatgttttaa atatatagag ggagaatcaa
      841 tattactact gcttgatcaa attggagttt gtgcatcaag cggatctgct tgcacttctg
      901 cctctcttaa accttctcat gtacttcttg caataggact acctgctgaa atagcacatg
      961 ggtctttaag acttacatta tcatatgaaa acacacaaga agaagttgat tacgttctta
     1021 atcagcttcc ctctattgta gaaaaattaa gagcattatc acctttaatt aaaaaataaa
     1081 aacatatgga acaatacagt aaaaaagtaa tggatcactt tcttaatcca agaaatgtag
     1141 gagaaattaa agatgcagac ggaataggag aggtaggaaa tcctgtatgt ggagatataa
     1201 tgagaatgta tatcaaagta aaagacaata taattacaga tataaaattc ctgacatacg
     1261 gatgcggtgc tgctgttgca acaagcagta tggtaacaga gatggtaaaa ggaaaaactt
     1321 tagaggaagc acttgagatc tctaataaac atgttgcaga agcactagat ggtcttccac
     1381 caataaagat gcactgctct aatcttgcag aggaaggaat taaagctgca atagatgatt
     1441 acaaaaagaa acatgacaaa aagtaatcct actgtacttc ttggaatgag cggaggtgta
     1501 gacagctcta caacagcata ctttcttaaa aagcagggct ttaatgtaac tggcattaca
     1561 ctttcaatgt ttaatgaaaa tgattcagat tttaagcaga atgtaaaaga tgctaaagaa
     1621 acatgcaaac tcttaggtat tccacatctt gtattcaatg tagaaaaaga ttttaaacaa
     1681 tatgttataa attacttcat atctgaatat gaagcaggaa gaacacctaa tccgtgtgta
     1741 atatgtaata gatatgtaaa gtttgaagga cttcttacta aagcagatga gcttggaatt
     1801 gaatatatat caacaggaca ctatgcttca gttgagaaaa aagatggtaa gtacctttta
     1861 aaaagaggca tagatgacaa aaaagatcaa agctactttc tttataacct tacacaagag
     1921 cagttatcaa gaagtatatt cccattagga caatatacaa aagaacagat aagagaaatt
     1981 gcaagaaaaa acaaattacc agcttcacac aaaagtgaaa gccaagaaat ctgttttata
     2041 aaagacaaca actacaagaa atatatagat gaaaacaaaa caaagaatat acctaaaggt
     2101 gaatttgttg atacaaaagg aaatataatc ggacatcata ctgggattca aaactataca
     2161 atcggacaaa gaagaaatct tggtatttct actggcaaac caatgtatgt actaagtata
     2221 gatggagaaa ataacagaat agtacttggt acaaataatg agctattctt aacacagctt
     2281 gttgctaaag acttgaattg gataatgatt gataaattag aaaaagaaat gaaagtcaaa
     2341 gcaaaggtaa ggtatggggc aaagcctgca gatgcaagaa tatctccatc gcaagacaat
     2401 agagtaaagg taatttttac aacagctcaa agagcgataa caaaaggaca agctgttgta
     2461 ttttatgata atcaatatgt tgtaggcgga ggaattatct cttagattag atatttgtat
     2521 aatctgtttt gctgtgctaa attatattat cattagcttt ttgaaacttt caaatgagca
     2581 gaagaaataa gaaaaaaaga gaaattaaaa aacagattca atctctaaga gctgtttccc
     2641 tctttttaac acttgtaata actgtattat ctacaagcat ttttctagtt agcaagaatc
     2701 ttcaaactga tgcacaggta aaaggagaaa gtactttttc aataaataac aaagtaaata
     2761 caagcagtta tacaaactat ttctcagata ttttaggcgt tgaatttata tatgataata
     2821 ctctctttac tatatctgaa aactccaact acattactct gtctacctta aatgggagcc
     2881 ttccatttaa agctggaaca atacgaaagg ttaaagataa agatataaag gatatatatc
     2941 cagattcaaa atatttagaa acaattaatg ataatggtgt aggaagaaca cttttttctt
     3001 acagtgtccc ttcaataata aaagaagaga atgaggaaac agagtattta actgtaatac
     3061 ataagtattt cccaaataac ataacaatat atatgcagat atggggatac aactttaaag
     3121 atgatttaag aacaacaagg gttttaaccg agctaatgga atctttaact ttagcaaccc
     3181 agaaatcaaa aaccagcggt gtactttctg caacctcttc aattgtaaat caagcacaga
     3241 tattaggcca ggcatcaact gtaaggatat acagtcaaaa gtgtaataat gttaagtttt
     3301 ctgattcttt aaagccatta agtgtaggag gaaat
//
LOCUS       DAOSAB010000052         3307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_19232_length_3307_cov_3.137761, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000052 DAOSAB010000000
VERSION     DAOSAB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19232_length_3307_cov_3.137761"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PK432_01495"
     CDS             <1..622
                     /locus_tag="PK432_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOK59630.1"
                     /translation="IGLIKLLGKMKYRTSYGQSLMSHTIEVIRIGEVLATELKADVDL
                     VKRACLLHDVGKVLSHKIDSPHHHISGQIARKYGLDEKLVNAIESHHLDIEPQSVEAV
                     IVYLADAISGARPGARKDSYEQYIQRIEALENVAKEVGGDKIEEVFAIRAGRELRVIV
                     KPNLISDDEMTVLAKDLAETIEKTQNYPGNVEVTLIRETRAVEIAK"
     gene            1086..1358
                     /locus_tag="PK432_01500"
     CDS             1086..1358
                     /locus_tag="PK432_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOK59631.1"
                     /translation="MLKRDLVEEVSKSAKITKKEAADAVDAVFEAITKALKAGDSVLL
                     TGFGKFEVRHRESRPGINPKTLESIKIPARTVPVFRAGKALKASVK"
     gene            1441..2907
                     /locus_tag="PK432_01505"
     CDS             1441..2907
                     /locus_tag="PK432_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOK59632.1"
                     /translation="MQAKLLTGSIYKDITYKLNRITAVKEYFIPNKKNNYKPYLLRKG
                     VLVIYTLIIFFVNTFGGLLGIPQAMASSITAENIIRLTNQERAAAGLNTLNTNAKLTA
                     AAQAKANNMFEVQYWDHYGPNGETPWMFITQAGYTYVYAGENLAKGFRTAEGVHEAWM
                     ASPTHRENIMSSNYKDIGVAVMEGTLLGKETILVVQMFGNQTTETFKPVSPTPKPSTT
                     TQPTPKPVIPEKGEIKSIRIVNPKNGDLINDANIDIKGDTQNVSGEYSVEIHNNGEFI
                     GETTATQSEWIFDKGSDWQEGEQNVSAKLKGEDVVSETVKFIIDSTPPQIDKETIKVQ
                     NGGSMFTLTFEAPQDAVDIQFVSDGKTHQIQKIEDSNIVSLDIPKSDIGDSSKLIMSD
                     QAGNIAEVDISEYFLEEVQGEKDKVTVAMSIKNVFGTTDGISLIVVSFVLILLILEAY
                     ISWKKGKLGKNAGDLFTIGAWWLILLVGIFNGFGGVIS"
     gene            2921..>3307
                     /locus_tag="PK432_01510"
     CDS             2921..>3307
                     /locus_tag="PK432_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59633.1"
                     /translation="MKNKVEVKKEIIYLFLVILLTVYFAYSTHTPNSIYILSGALGNI
                     LILTWFTTAFVRYSGRRISQYSEVIQRISLKERFFTYFILPLLFYNSLLAYVYFNTSF
                     TMDLVLIAISSILLFILFLNVKSSFKK"
BASE COUNT         1202 a          456 c          624 g         1025 t
ORIGIN      
        1 tatcgggttg ataaaactgc ttggaaaaat gaagtataga acaagctatg gtcaatccct
       61 tatgtcccat actattgagg taattagaat aggagaggtt ttagctactg aattaaaagc
      121 agatgttgat ctagtaaaaa gagcatgtct tttgcatgat gttggaaagg ttctttccca
      181 taaaatagat agtccgcatc atcatatatc tggtcaaatc gcaagaaaat atggactaga
      241 tgagaaactt gttaatgcaa tagagtcaca tcacttagat attgaaccac agtctgttga
      301 agcagtaatt gtatatcttg cagatgcaat atcaggtgca agaccagggg ctagaaagga
      361 tagttatgaa cagtatattc aaagaattga agctttagag aatgttgcaa aggaagtagg
      421 cggagataag atagaggagg tgtttgcaat aagggcagga agagaattaa gagtaattgt
      481 aaagccaaac ttaatttctg atgatgaaat gacagtactt gctaaggacc ttgcagaaac
      541 catagaaaag actcaaaact accctggtaa tgttgaagtt actcttatta gagagacaag
      601 agctgttgaa atagctaaat aattgaagaa taactgataa cttctagtat gcaatactac
      661 aggttgtttt agttgtttta agaaagagag tagaaaaata tctactctct ttttattttt
      721 aatttaagaa tgttcaaatt ttatcaatac tttctaacat aactcccctt ttatttattt
      781 taaattgttg ttaactttcc tttatattaa ggcatttttg caagatatgt tgaaataaca
      841 aggaatcttt ttaaaatcac attttatttt taaattggat tataaaaagt ttgaacagta
      901 gaaactcaaa aaaagtcttt tatttaatga atttctttaa taattttcat ttaccctata
      961 gaaaagatat tcttgacgaa gtcttgtatt taccatattt tattgatgtg atactacaaa
     1021 tcatctgata tcaggatttg tggcatccat atctcttaga aaggaggtaa agaaaaatgg
     1081 caacaatgtt aaaaagagat ttagtagagg aagtttcaaa gtctgctaag attacaaaga
     1141 aagaggcagc tgatgcagta gatgcagtat ttgaagcaat tacaaaagct ctaaaagctg
     1201 gtgattcagt acttttaaca ggttttggta aatttgaagt aagacacaga gaatcaagac
     1261 ctggaatcaa ccctaagact ttagagagta ttaaaattcc tgcaagaact gttcctgtat
     1321 tcagagcagg aaaagctcta aaggcatctg taaagtaata aaactgccgg cgtttctctt
     1381 tagataagag aaaagaagta gcctgaggat atctcaggct tttcttttga taatatccct
     1441 ttgcaagcaa agcttttaac tggtagtata tacaaggata ttacttacaa attaaacaga
     1501 ataactgccg tgaaggaata ttttattcca aacaaaaaaa ataattacaa accatatcta
     1561 ctaagaaagg gtgtcttagt tatatacacc ctgattatat ttttcgttaa cacatttgga
     1621 ggtcttcttg gaataccaca ggcaatggca agtagtatta cagcagaaaa tattatcaga
     1681 cttactaatc aggaaagagc agctgccgga ttaaacacat taaacacaaa tgctaagctt
     1741 actgctgctg cacaggcaaa agcaaataac atgtttgaag ttcaatattg ggatcactat
     1801 ggaccaaatg gagaaactcc ctggatgttt ataactcagg caggatatac atatgtatat
     1861 gcaggagaga acctcgcaaa gggctttaga acagcagagg gtgtacatga ggcatggatg
     1921 gcaagtccta cacacagaga gaatataatg agttctaatt acaaagatat tggtgtagct
     1981 gtaatggaag gaacattact tgggaaagaa actattcttg ttgttcaaat gtttggtaat
     2041 cagactactg aaacatttaa accagtatca ccaactccaa agccatctac tacaacacag
     2101 ccaactccta aaccagtaat ccctgaaaaa ggagagatta agtctataag gatagttaat
     2161 cctaaaaatg gagatttaat aaatgatgca aatatagata ttaaaggtga tacacagaat
     2221 gtgtctggag aatattctgt tgagatacat aacaatggag agtttatagg agagactaca
     2281 gctactcaaa gcgaatggat atttgataaa ggaagtgatt ggcaagaagg agaacaaaat
     2341 gtttctgcta agcttaaagg tgaagatgta gtgtctgaaa ctgtaaaatt catcatagat
     2401 tcaactcctc ctcaaattga taaagagaca ataaaggtac aaaatggtgg cagtatgttt
     2461 acacttacat ttgaagcacc acaggatgct gttgatattc agtttgtaag cgacggaaag
     2521 acacatcaaa tacaaaaaat agaagatagt aatatagtat cgttggatat accaaaaagc
     2581 gatataggag atagctctaa gttaattatg tctgatcaag ctggaaatat tgcagaggta
     2641 gatatttctg agtacttcct agaagaggtt cagggagaga aagataaggt aacagttgcg
     2701 atgagcatta agaatgtatt tggtactact gatggaatta gtttaattgt tgtttctttt
     2761 gttcttatcc ttcttatcct tgaagcatat atatcatgga agaagggaaa gcttgggaaa
     2821 aatgctggtg atttatttac aataggggca tggtggttga tacttttagt aggtatattt
     2881 aatggattcg gtggagttat aagttaagta ttttattgaa ttgaaaaata aagtagaagt
     2941 taaaaaagag attatatatt tatttctagt tatactttta actgtatatt ttgcatactc
     3001 aacacatact cctaattcaa tatatattct aagtggagca ttaggaaata ttttgatact
     3061 tacatggttt acaactgctt ttgtaagata ttctggaaga agaatatctc agtatagcga
     3121 agtaattcaa agaatatccc ttaaagaaag attttttact tactttattc ttcctcttct
     3181 cttttacaat tctcttctag catatgtata ctttaataca agctttacaa tggatcttgt
     3241 actgatagcc atatcatcta tacttctatt tatacttttt cttaatgtta aaagctcttt
     3301 taagaag
//
LOCUS       DAOSAB010000053         3295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_19330_length_3295_cov_2.460802, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000053 DAOSAB010000000
VERSION     DAOSAB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19330_length_3295_cov_2.460802"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PK432_01515"
     CDS             <1..337
                     /locus_tag="PK432_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59634.1"
                     /translation="CTSLGCIKWFNDQDEKHDTIESIYSAFNQERPFGEVFYRFCIGG
                     KEYTNRGGYKAHELFLPKYSKEGTPIEKDDVQVSKLIKYNPEEHIRRKKEEKVEGFGE
                     AGAAQNFEL"
     gene            390..602
                     /locus_tag="PK432_01520"
     CDS             390..602
                     /locus_tag="PK432_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59635.1"
                     /translation="MKLLDYEKKLVLREHDVKKIVLRYIRYGTMSIWDIGISDLYKFY
                     YTDKQLKHCTEKGTLYYLISMGKLDY"
     gene            619..1647
                     /locus_tag="PK432_01525"
     CDS             619..1647
                     /locus_tag="PK432_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOK59636.1"
                     /translation="MLRTKLLITDITEVPREWIFEHYLQLQEKLTGQDVKIKSVFNKN
                     ERTPSMCIYYASNNTYKYKDFSTGKSGDAINLVMEITTPKLTTRGETAHKIIEDYNSF
                     LLNSKDGYEMRNFKKRSKYKVVDFTTRSWSNFDEKYWGKFHINSKLLEFYNVTALSEY
                     KLQKEDENGEIVEQVIKSTNMYGYFRKDGTLYKIYHPLVKDYKFIKVQDYIQGMDQLT
                     MKVPYLIICSSLKDIMAFKKLGYNNAEAIAPDSENTMIQEHVIIALKHKYKSICTLFD
                     NDPAGLESMKKYEEKYELPYVVLPMSKDLSDSVRDTTLTKVRETLTPLLKEVFIKRLK
                     TNELDISG"
     gene            1625..2059
                     /locus_tag="PK432_01530"
     CDS             1625..2059
                     /locus_tag="PK432_01530"
                     /inference="COORDINATES: protein motif:HMM:NF040378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59637.1"
                     /translation="MSWIYQGKPFRDEDIPPGAIGFIYMIEAIIDGKSVAYIGKKNFQ
                     ALVKKKLSKKNLPKDKRKKQYEHVAKTAYNNYYSSNEVLKKAQQDGVLMKRTMLKICY
                     SKTELTYQEVKHQFKYEVLEDDKFLNGNILGSFYKQKKHEQG"
     gene            2046..2462
                     /locus_tag="PK432_01535"
     CDS             2046..2462
                     /locus_tag="PK432_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59638.1"
                     /translation="MNKDEKITIINESQYESLLKMLMASEEDALLAFNILNQVDLSQN
                     LLQTALLRKESGWEFSAWEMHCPTIVQYHNYHGIPSIPDFKNIYNLFVRAPDHMNKEG
                     KIIYLRRYIKDVERLLRSIPLVEHIEINFKIKEDEQ"
     gene            2452..2697
                     /locus_tag="PK432_01540"
     CDS             2452..2697
                     /locus_tag="PK432_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59639.1"
                     /translation="MSNKTDELAKASKDLMLKEPFYGLFLIMLNKQWNNKVVPTASVS
                     RQGVNYQLYLNEEFWESLSANQHMGLLKHELKGLLVQ"
BASE COUNT         1274 a          450 c          612 g          959 t
ORIGIN      
        1 atgtacaagt cttggatgta taaagtggtt caatgatcaa gatgaaaaac atgatacaat
       61 tgaatctatt tatagtgcat ttaatcaaga aagacctttt ggtgaagtat tctacagatt
      121 ctgtattggc ggcaaggaat ataccaaccg tggaggctac aaagcccatg aattgttctt
      181 acctaaatat tctaaagaag gaacacctat tgagaaagat gatgtacaag tatctaagct
      241 tattaagtat aatcctgagg agcatattcg cagaaagaaa gaagaaaaag ttgaaggatt
      301 tggagaggca ggagctgctc agaattttga attataaatt aaaaaagggg ggtaaatata
      361 gctccccttt tgtttaaact tcaatcatca tgaaactatt agattatgaa aaaaaactag
      421 tattaaggga gcatgatgta aaaaaaattg ttttaagata tataagatat ggaacaatgt
      481 ccatctggga tattggcata tctgatcttt acaaatttta ttatacagat aaacagttaa
      541 aacattgtac tgaaaaaggt accctgtact acttaatatc aatgggaaaa ctagattact
      601 aatttttgat aggccaatat gttaagaact aaattattaa ttacagacat aacagaggtt
      661 ccaagagaat ggatatttga acactattta caactgcagg aaaagttaac tggtcaggat
      721 gtaaagataa agtctgtgtt caataagaat gaaagaactc cctcaatgtg tatatattat
      781 gcatctaata atacttataa gtataaggat ttctcaacag ggaaatccgg agatgcaatc
      841 aatcttgtaa tggagattac tactcctaaa ttaactacaa gaggtgaaac agctcataag
      901 ataattgaag actacaatag ctttctactt aatagcaaag acggctatga gatgagaaat
      961 ttcaagaaga gatccaagta taaggtagta gattttacta caagatcttg gagtaacttt
     1021 gatgaaaagt attggggtaa atttcatatt aattccaagc tgctagaatt ctataatgtt
     1081 acagcattat cagaatataa acttcaaaaa gaagatgaaa atggtgagat tgtagaacaa
     1141 gtaataaaga gtactaatat gtatggttac ttccgcaaag atggaactct atataagatt
     1201 tatcatccac tagtaaagga ttataaattt atcaaagtac aggactatat ccaaggcatg
     1261 gatcaactca caatgaaggt accgtatttg attatctgca gttctctaaa ggatatcatg
     1321 gcttttaaga agctaggata taataatgca gaagctatcg cgccggacag tgagaatact
     1381 atgatacaag agcatgtaat aatagcgctt aaacacaagt ataaatctat ttgtacttta
     1441 tttgataatg atcctgcagg attagaatca atgaagaagt atgaagagaa atatgaactg
     1501 ccttatgtag tattacctat gtctaaggac ttatcagatt ctgtaagaga tactacacta
     1561 acaaaagtta gagaaactct tacaccatta ttaaaagaag tatttataaa aagattgaag
     1621 actaatgagt tggatatatc agggtaaacc atttagagat gaagatattc ctccaggagc
     1681 tataggattt atctacatga tagaagctat catagatggt aaatctgtag cttacatagg
     1741 taagaagaac ttccaagctc ttgtgaaaaa gaagctgagt aagaagaatc tacctaagga
     1801 taagaggaag aagcagtatg aacatgtggc taagaccgca tataataatt actatagtag
     1861 taatgaagtg cttaagaaag cacagcaaga cggagtatta atgaagagaa ccatgctcaa
     1921 aatatgttat agcaaaactg aattaactta ccaggaggta aagcatcagt ttaaatatga
     1981 ggtattggag gatgacaaat tccttaatgg gaatattcta ggctcttttt acaaacagaa
     2041 aaaacatgaa caaggatgaa aaaataacaa tcataaatga atctcaatat gaaagcttgc
     2101 tcaaaatgct tatggcctcg gaagaggacg cattactagc atttaatata ttaaatcaag
     2161 tagatctttc tcaaaatctt ttacaaacag cacttctaag gaaggaatcc ggctgggaat
     2221 ttagcgcatg ggaaatgcat tgtccaacta ttgtgcaata tcataattac catggtattc
     2281 cttcaatacc tgattttaag aatatttaca atctttttgt aagagcacct gatcatatga
     2341 ataaagaagg aaaaataata tacttgagaa gatatattaa agatgtagag agacttctaa
     2401 ggtcaatacc tctagtagag catatagaga tcaattttaa aattaaagaa gatgagcaat
     2461 aaaacagatg aactggcaaa ggccagtaaa gatctaatgc tgaaagaacc cttctatgga
     2521 ttattcctga ttatgctgaa taagcaatgg aataataaag ttgtgccaac agccagcgta
     2581 agtagacaag gggtaaacta tcagctatac ttaaatgaag aattttggga aagtctgagt
     2641 gctaatcaac acatgggctt actaaagcac gagctcaaag ggctccttgt gcagtaatgt
     2701 acatgtaaaa aaggtttaaa ttgcgggaag ttccttaagt tttatccact aactaatcat
     2761 agtaatatag attagggcac tgctaattac agtggtagag taaatgtgat aaaaattgga
     2821 taatctgcag ccaaatttct tggataatta aattattagt ggtaataaga gtttatatat
     2881 tattcaattc tctctaatgg acagtattgc tttacataat tatatgtata aagactttac
     2941 aatttgtctt gttagaaaat ttttaatatt tagtcattac ttattagaat acaccaagaa
     3001 agaggttcaa cgactataat aacctaccct aatagggtaa agggatagtc tgatcttaca
     3061 tgaaagtgta agttaacaca aatgattgca tatagcattc tttcatatta ctgattatga
     3121 tcatctcacg gataaggaaa ttgctaatat agcaatggat taactcttag tcccgctgta
     3181 cagtaatgta tagtagaaag tcttaaattg ccggggagtt ccttagaact ttacacacca
     3241 agcattcata gtaatataga atgtggccag aataattaac tgggtatggt aaaag
//
LOCUS       DAOSAB010000054         3282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_19432_length_3282_cov_3.631236, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000054 DAOSAB010000000
VERSION     DAOSAB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19432_length_3282_cov_3.631236"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2617)
                     /locus_tag="PK432_01545"
     CDS             complement(<1..2617)
                     /locus_tag="PK432_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59640.1"
                     /translation="MADCKSFYNQYVAIFHELLAMDNQFSNWDYLMEYLLKADLPQDQ
                     LAMSIQVFGAIYDRLNISKPAIVTMGNWKQLNESIAKIYATPNQTQELISFNISNAAK
                     SLYNKETKKDYNKRLEELTNILNNEDIILITNVQEIANIFIEQLKRYANEPDKLQKVK
                     NNIDSISKLILSKDKAKGLLFNQMINITTDGNKNYKALDTVEGVGTYQGKPIAWILTN
                     DYRQTYVIVDGNRFYEVIYNNNGITIGDEINGKDIKIIKSPRRSNEVKFHKQEGKTFV
                     ANDTISDGMVLRPLDESKEGLERFSKMISTATGIEKIMAVKVSEEAIERTNYIKDTIK
                     GRQHETFETLGQQSYLEHNPDGVVVTVSRPNKGVIFIAENKETIASLYGLSNFVFVTA
                     DNKTTKVDFHNPEHLKLLQTRAVKADNSKLSYSEILEMQRLSDLYDEFSKEVENKIGD
                     NNAIDVTDIFNKYYTIELQNNVKELWTIEKRIEAVFESKYSNPDVREYDIPLNIVQVD
                     TNGKIIMDLGIQNIPFVMFYDKLQGEFVVVSPLDDDQFIYENGELSKPDEYFKSKRFK
                     NKTWYEHITKGQEVNPSTENMFIVTWARPTKETGFIPRTYQIVEMLPVSRQASNIAFT
                     IGSLFELVNSTGTIKEAKVANINKEGFEFNVVKALGKLTPILNVKFGISSLTKKATIY
                     LKPYAGSKYEDLFQRIKENKVQDIISFELDEVRIKKLFENIINSKVVSNFQESAPFVH
                     QGKIETKEQLDSIVKAAEKLNSKEAALLLESMDKVHNELVSIFDDALVKTLLSERTFT
                     GDKADIKNELYKELLDGLKEDFKLKNGSFAPQFILFKQIPNTNTEYSLNLNYKENTRN
                     SKLIRDVRILQRPYDK"
     gene            complement(2621..>3282)
                     /locus_tag="PK432_01550"
     CDS             complement(2621..>3282)
                     /locus_tag="PK432_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59641.1"
                     /translation="INAKYDAELAALNNQTASEQKQQAQETQEESQSAEQQKVGIEQS
                     PEIQEKETPLNTDLFPTQSIEELNITYARKKAEQIIEELPEDLLSFIENIEDAKLYLQ
                     NIILERLVKYTGKDIGSITDKRSTKAIDRIINKIKQEFFNLMEQERTIVEVESVPDSD
                     ITNVDVLSIEGIIEQVHQNTATLVESTEKILANSKKNSNIVATEEAILNVLKEIKNFK
                     C"
BASE COUNT         1012 a          492 c          356 g         1422 t
ORIGIN      
        1 ctttatcata aggtctttgt aatattctta catctcttat taatttagaa tttcttgtat
       61 tttctttata attaaggttt agactatatt ctgtattagt attagggatc tgtttaaata
      121 aaataaattg tggagcaaaa ctaccattct ttaatttaaa atcttctttt aaaccatcaa
      181 gtaattcttt atataattca tttttaatat cagccttatc tcctgtgaaa gttctttcag
      241 ataataaggt ttttactaaa gcatcatcaa atatagacac aagttcatta tgtactttat
      301 ccatactttc tagtaataaa gcagcttctt tactatttaa tttttcagca gctttaacta
      361 tactatcaag ttgttcttta gtttctattt taccttgatg aacaaatggt gctgattctt
      421 gaaaattaga tacaacttta gaattaataa tgttttcaaa taattttttt attcttactt
      481 catctaactc aaaagatata atatcttgaa ctttattttc ttttattctt tgaaataaat
      541 cttcatattt actaccagca taaggtttta aatatattgt agctttttta gtaagactac
      601 ttattccaaa ctttacattt agtattggag ttagcttgcc taatgctttt actacattaa
      661 attcaaatcc ttctttattt atatttgcta cttttgcttc tttaatagta cctgttgaat
      721 ttactaattc aaataaagaa cctattgtaa atgcaatatt tgaagcttgt cttgatacag
      781 gtagcatttc tactatttgg taagttcttg gaataaaccc tgtttctttt gtaggtcttg
      841 cccaagtaac aataaacata ttttctgtac taggattaac ctcttgtcct ttagtaatat
      901 gttcatacca agttttattt ttaaatcttt ttgatttaaa atattcatct ggtttactaa
      961 gttcaccatt ttcataaata aattgatcat cgtctaatgg agatacaact acaaactcac
     1021 cttgtagttt atcataaaac attacaaatg gaatattttg tattcctaaa tccataatga
     1081 tttttccatt tgtatcaact tgtactatgt ttaaaggaat gtcatactct cttacatcag
     1141 gattagaata tttactttca aatacagctt ctattctttt ttcaatagtc cataattcct
     1201 ttacattatt ctgtaattct atagtataat atttattaaa tatatctgta acgtctattg
     1261 cattattatc tccaatttta ttttctactt ctttagaaaa ttcatcatat aaatcagata
     1321 atctttgcat ttctaatatc tcagaataac taagtttaga gttatctgct tttacagctc
     1381 tagtttgtaa taatttaaga tgttcaggat tatgaaaatc aactttagtt gttttattat
     1441 ctgctgtaac aaatacaaaa tttgataatc catataaact agcaatagtt tctttatttt
     1501 ctgctataaa tataactcct ttatttggtc ttgatacagt tacaacaact ccgtcaggat
     1561 tatgttctaa ataagattgt tgtcctaaag tttcaaatgt ctcatgttgt cttcctttaa
     1621 tagtatcttt aatataatta gttctttcta tagcctcttc agagaccttt acagccatta
     1681 ttttttcaat gcctgttgca gtactaatca ttttactaaa tctctctaaa ccttctttag
     1741 actcatccag cggtcttaat accataccat cagatatagt atcattagct acaaaagttt
     1801 tgccttcttg tttgtgaaat tttacttcat tacttcttct tggactttta atgattttaa
     1861 tatctttacc atttatttca tcaccaatag taattccatt attattataa ataacttcat
     1921 aaaatctatt accatctaca ataacatatg tttgtctata atcattagtt aatatccaag
     1981 caataggttt accttgatat gttcctacac cttctacagt atctaaagct ttataatttt
     2041 tattaccatc tgtagtaata tttatcattt gattaaaaag taaaccttta gctttatctt
     2101 tagataaaat aagtttagaa atagaatcta tattgttctt tactttttgt aatttatcag
     2161 gttcattagc atatcttttt aattgttcta taaaaatatt agcaatttct tgtacattag
     2221 taattaaaat aatatcttca ttattcaaaa tatttgtaag ctcttcaagt cttttattat
     2281 aatctttttt agtttcctta ttatataaac ttttagcagc attagaaata ttaaagctaa
     2341 tgagttcttg tgtttgattt ggtgttgcat atattttagc aatggattca tttaactgtt
     2401 tccaattacc cattgtaaca atagcaggtt ttgatatgtt taatctatca taaatagcac
     2461 caaacacttg aatagacatt gctaattggt cttgtggtaa atctgctttt aacaaatact
     2521 ccattaaata atcccaatta gaaaattgat tatccattgc taaaagttca tggaatatag
     2581 ccacatactg attataaaaa gatttgcaat ctgccatatt ttaacattta aagtttttaa
     2641 tttcttttag tacattgaga atagcttctt ctgttgctac aatgttacta tttttttttg
     2701 aattagcaag tattttttct gtactttcta caagagtagc tgtattttga tgtacttgct
     2761 ctataatacc ttctattgat aatacatcta catttgtaat atcactatca ggaacagatt
     2821 caacttctac tatagttctt tcttgttcca ttaaattaaa aaattcttgt tttattttat
     2881 ttattattct gtcaatagcc ttagtacttc ttttatctgt aatactacca atgtcttttc
     2941 ctgtatattt tacaagtctt tctaatataa tattttgtaa atataattta gcatcttcaa
     3001 tgttttctat aaatgataat aaatcttcag gtaattcttc tattatttgt tctgcttttt
     3061 ttctagcata agtaatatta agttcttcta tagattgtgt aggaaataaa tcagtattta
     3121 aaggagtttc tttttcttgt atttcaggag attgttctat accaactttt tgttgttctg
     3181 ctgactggga ttcttcttgg gtttcctgtg cttgttgctt ttgttcagaa gctgtttggt
     3241 tgtttaaagc agctaattct gcgtcatatt tagcattgat tt
//
LOCUS       DAOSAB010000055         3255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_19643_length_3255_cov_3.249062, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000055 DAOSAB010000000
VERSION     DAOSAB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19643_length_3255_cov_3.249062"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1476)
                     /locus_tag="PK432_01555"
     CDS             complement(25..1476)
                     /locus_tag="PK432_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59642.1"
                     /translation="MESKEIIKILNEIEEKFPVDKWSVDDLDIWPYVRFRIGSKLSES
                     KSPSQKNVLYERYIKNLYKYYKNNLKNWLKNKDKNVDFLFVENGVHIADIKGKYYFTR
                     TDPLRDKIEKSGYKCKTIIGGYKVETPLYNSGEFFQFSLDMMFVLDKVKNCFKKPPLL
                     NYKLESYSEFINFLKQNNIYLEELELKNLLGQVSRIKRIEKYFTERLKKLKPKAVFIL
                     CYYSTYGFALVSSCKKLAIPVIDIQHGVQGDLHYAYGSYKKVPKNGFNLHPDIFYVWS
                     DEEKSSLLKWIKGNTKVFVGGNNFLEMWKDDGNEIVQYYTNMLKEKYLISKYSKVILY
                     TVDPQNNRDKEIIKAIQHSPSDWFWFVRVHPKRVDMLEYTKKRMGDTKCGFAIDNIYL
                     LPLYSFFKVCDVHITERSSTIIEAANFGIKSIVTSKYSLEIFKEYFDSGMAVYGSSWK
                     EILKYIKRFKREDNIKKRKEVDILELVKMIEEI"
     gene            complement(1442..2920)
                     /locus_tag="PK432_01560"
     CDS             complement(1442..2920)
                     /locus_tag="PK432_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOK59643.1"
                     /translation="MSVIKNTTIYFSVSIIQSLISFLLLPVFTYFLTKAEFGLVSVVN
                     SISALLAIFFIFGTQAVVSRLYFEYKDDEEKLKTFLGTVFLSKIIWNILLAAVLILGR
                     NIIFPIIAKDINFYPFLLIAIGIGFFSVIFKIYQTLQQTEQKSKSYAIDQILYLVLNN
                     GITVLLLVVFDMKAEGVILGTLIADFIMTVLVFFRLRKRMILKIDRDILKESFSYSWP
                     IFFHALFGWGLASVNRLILNNIVSIETVGVYTIGFTIAGVVNMITVALNHSYTPWFYS
                     QMKKEKRDNGNIVRFAEFIILIYSILSLCISLFSPEVIYLFINKGYVQAWKVIPFISF
                     GYVFNGLYFFFINIFNYTKKAVKYVPLYSLFCALINIGLNYALIPRYGMIGSALATMI
                     SMFVLSLSTYIGSKRFIDVGFKYKRMISLILLPFLMSLFVFIDFNLNFWVVLIIKIIY
                     VLICIFILLLVNRKQFGDSIAHQIDNLKEYWHGIKGDNKNSK"
     gene            complement(2923..>3255)
                     /locus_tag="PK432_01565"
     CDS             complement(2923..>3255)
                     /locus_tag="PK432_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOK59644.1"
                     /translation="NEYIEKSKLLLMTSKSEGLPMVVVECMAYGVPAVVPNVGDIKDL
                     VIQGENGIIVNTRDPKEFAKEIVNLLKDKKRYREMSLECIESIKKIVNNTSHDKLVEL
                     WDSLLKDI"
BASE COUNT         1162 a          607 c          352 g         1134 t
ORIGIN      
        1 cattttaaaa acatctaaat ttaactaaat ctcttcaatc atttttacaa gctctaaaat
       61 atctacctct tttctctttt taatattatc ctctctttta aatcttttaa tatattttag
      121 aatttccttc caactagagc cgtatacagc cattccgcta tcaaaatact ctttaaatat
      181 ctcaaggctg tattttgatg taacaattga ttttatacca aaattagctg cttcaattat
      241 agtagaagac ctctctgtta tatgaacatc acaaacctta aaaaaagaat acaaaggtaa
      301 gagataaata ttatcaattg caaaaccaca ctttgtatct cccattcttt tcttagtata
      361 ttcaagcata tctactctct taggatgaac tctaacaaac caaaaccaat cagaaggact
      421 atgctgtatt gcctttataa tctccttatc acgattattc tgaggatcaa ctgtataaag
      481 aattactttt gaatatttac taatcaagta tttctctttt agcatattag tatagtattg
      541 aactatctca tttccatcat ccttccacat ttccaaaaaa ttgtttcctc ctacaaacac
      601 ctttgtattt ccttttatcc attttaataa tgagctcttc tcttcatcac tccatacata
      661 gaagatatca ggatgtagat taaacccatt tttaggaact tttttgtaac taccatatgc
      721 ataatgcaaa tctccttgta ctccatgttg aatatcaatt acaggaatag ccaatttctt
      781 acatgaagac acaagagcaa aaccatatgt actgtaataa caaagtatga aaactgcttt
      841 aggttttaat ttcttcaacc tctcagtaaa atacttctct atcctcttta ttctactcac
      901 ttgccctaaa agattcttta actcaagctc ctctaaatat atattatttt gtttaagaaa
      961 attaataaat tcactataac tctcaagttt gtaatttaat aaaggaggct ttttaaaaca
     1021 atttttcact ttatcaagta caaacatcat atccaagctg aattgaaaaa actctccact
     1081 gttatacaaa ggtgtctcca ccttataccc tcctattatt gtcttacatt tataacctga
     1141 tttttcaatt ttatccctta gcggatctgt tctagtaaag taatacttcc ccttaatatc
     1201 tgcaatatga acaccattct caacaaacaa aaaatcaaca tttttgtctt tgttctttaa
     1261 ccaattctta agattattct tgtaatactt ataaagattc ttaatatatc tctcataaag
     1321 gacattcttt tgtgatggag atttgctctc agaaagtttt gatccaatcc taaaccttac
     1381 atatggccat atatctaaat catcaacact ccatttatct actgggaatt tctcttcaat
     1441 ttcatttaga atttttatta tctcctttga ttccatgcca atattctttt agattatcta
     1501 tttgatgtgc aatactgtct ccaaactgct ttctatttac taacagcaga ataaagatac
     1561 aaatcagtac atatatgatt ttaataatta aaacaaccca aaaatttaag ttaaaatcta
     1621 taaatacaaa caaagacatt aaaaaaggaa gaagtattaa agatatcatc cttttgtact
     1681 taaatcctac atcaataaat cttttagacc ctatatatgt acttaaactt aaaacaaaca
     1741 tagatatcat tgttgcaagt gcagaaccaa tcattccata cctagggatt aatgcatagt
     1801 ttaatccaat atttattagt gcacagaaaa gagagtatag cggaacatac ttaaccgcct
     1861 tcttcgtata gttaaagatg ttaataaaaa agaaatacaa accattaaat acatatccaa
     1921 atgatatgaa aggaattact ttccatgctt gtacataccc cttatttata aacaagtata
     1981 ttacttcagg agaaaataga gaaatacata aagaaagaat tgaatagata aggatgataa
     2041 actcagcaaa tctaacaata ttaccattat ccctcttctc ttttttcatc tggctataaa
     2101 accaaggagt ataggaatga tttagtgcaa cagtaatcat gttaacaact cctgctattg
     2161 taaaaccaat tgtatacaca cctactgttt caatagatac aatattatta agtattagtc
     2221 tgtttacaga agccaatccc catccaaaga gtgcatgaaa gaatatcggc caagagtatg
     2281 agaaagattc tttcagaatg tctctgtcta ttttaagaat cattctcttt cttaatctga
     2341 aaaacacaag tacagtcata atgaaatctg caataagagt acctaatatt actccttctg
     2401 ctttcatatc aaatacaacc aggagcaaaa ctgtaattcc attattaagc acaagataca
     2461 atatctgatc aattgcatag cttttgctct tctgctctgt ttgttgcagt gtctgatata
     2521 ttttaaatat cacagagaaa aatcctattc ctattgcaat taaaagaaat ggataaaaat
     2581 taatgtcttt agcaataatg gggaatataa tgttccttcc tagaataaga actgcagcta
     2641 aaagaatatt ccatataatt ttagaaagaa atactgttcc taagaatgtt ttaagctttt
     2701 cttcatcatc tttgtattca aaatacagtc tgcttacaac agcttgagta ccaaatataa
     2761 agaagatggc aagtagtgct gatattgaat taacaacact tactaatcca aactcagcct
     2821 tagtaagaaa atatgtaaat acaggtaaaa gtaaaaagct tatcaatgac tgaataatac
     2881 ttacactaaa atatattgtt gtattcttaa ttactgacat agctatatat cttttaataa
     2941 actatcccac agctctacca gcttgtcatg tgatgtgtta ttcactattt tctttattga
     3001 ttctatacac tccaatgaca tttctctata cctcttttta tcttttaaga gatttacaat
     3061 ctccttagca aattccttag gatctcttgt atttacaatt attccattct ccccttgtat
     3121 aaccaaatct ttaatatcgc cgacattagg aacaacagca ggaacaccat atgccataca
     3181 ttcaacaaca accataggaa gaccttcact tttagatgtc attagaagta gtttagactt
     3241 ctcaatatat tcatt
//
LOCUS       DAOSAB010000056         3255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_19647_length_3255_cov_2.863437, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000056 DAOSAB010000000
VERSION     DAOSAB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19647_length_3255_cov_2.863437"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /gene="pheS"
                     /locus_tag="PK432_01570"
     CDS             <1..712
                     /gene="pheS"
                     /locus_tag="PK432_01570"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOK59645.1"
                     /translation="GSSHPIAQELKKMLDIFTSMGFDILESRQIDDDFHMFESLNFPK
                     GHPARDMYDTFWTDENFVLPAHTSTMQNRALRRFSPPPIRVVLPGRCFRNEATDASHE
                     HTFYQIEGIYVDENISMSNMQATIKEYLEKYLETKLEIKMQPAYFPFTEPDTEFVISC
                     PFCKKKGCSTCGYSGWIELMGCGMIHPNVLTAGGIDPQKYSGFAWGVGVDRLVAIKRK
                     ITDIRKLRDGDINFLRQF"
     gene            716..3187
                     /gene="pheT"
                     /locus_tag="PK432_01575"
     CDS             716..3187
                     /gene="pheT"
                     /locus_tag="PK432_01575"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOK59646.1"
                     /translation="MKLPIKSIKEFGKCTLSTEEIVNIVSTKIGEVEEVTDFSKIYEG
                     IVIAQITKKEDHPNADKLGVYMINIGEEKEIQVVAGDKSLNVGDKVAYITVGHIVPYS
                     YATSQPVKIKAVNMRGITSNGMLCSQKELNIGPDDTHVLVLEKDAPVGVPFAQYYQFD
                     DVVVGIENKALTNRGDLFGILGLAREISAAQNIPFKSPKWYLKTTSDIDVTETLPIKV
                     DNQMQNLCPRYTCIAIKDVQIKESPVWMKSVLLKSGIKPINNIVDITNYLSVITAQPL
                     HAFDYDKLVENDLTRDGTAHITIRLAKNGERIHTLEGNIVELSEDNLVIADSTNPIAI
                     AGVIGGIDTQIDNNTKNIVIESANFNKYSIRKTSMELGIFTEAVTRFTRGQAPQLSLQ
                     ILLKAAELVQELANGEVASNVIDIYPEPAKPKKLSLSVSRLNSHLGTELSKEEIKNIL
                     LNIEYEIIKEDDEYLTIIPPVFRTDISIPEDIYEDVGRIYGYENILPKLPTREINAVV
                     FNEKIALKEKIRNILTYSGCNELDTYSFTSKESLQNAKQDPNFAYHIKNALSPELEFM
                     RISILTSLLQKAKENIQKNISPLCIYEFNIPHQKGYMDNFQLPKEDWHLSMVFSSKEK
                     IVDGSPYYQIKRYAEKIFNSVGVENISYDLVSLSSQMDLPIWIKCIMPTFNPNQSALI
                     KYHTDGTESIIGIVGDIDYEVKNNFKLPDFTAALEINLEELIKIIKPKTIRVFDSKFP
                     SISQDICFTVPSSTKYCDIENSVLQIINTRDRVATIECIDVYQKSEHSKNVTLRITIE
                     HQNKTLSDKEFSKIKEKIEERIKHL"
BASE COUNT         1271 a          472 c          581 g          931 t
ORIGIN      
        1 aggttcttct cacccaattg cacaagaatt aaaaaaaatg cttgatatat ttacaagtat
       61 gggatttgat attctagaaa gcagacaaat agatgatgac tttcatatgt ttgaaagttt
      121 aaacttccct aaaggacacc ctgcccgaga catgtatgac acattttgga cagatgagaa
      181 ttttgtactt cctgcacata cctctacaat gcaaaacaga gctttaagaa gatttagtcc
      241 tccccccatt agagttgtac ttccagggag atgttttaga aacgaagcaa ctgatgcaag
      301 ccatgaacat acattctatc aaatagaagg aatatatgta gatgaaaata tatcaatgag
      361 taatatgcag gcaacaataa aagagtattt agaaaagtat cttgaaacaa aacttgaaat
      421 taaaatgcag cctgcatatt ttccatttac agaacctgat accgaatttg taataagctg
      481 tccattttgt aaaaagaaag gatgtagtac atgcggatat agcggatgga tagaattaat
      541 gggatgcgga atgattcacc ctaatgtctt aactgcagga ggaatagatc cacaaaagta
      601 tagcggtttt gcatggggag taggggtaga taggcttgtt gcaattaaaa gaaaaataac
      661 tgatattcgt aagctaagag atggagatat taatttctta agacagtttt aatatatgaa
      721 attacctatt aagagtataa aagaatttgg caaatgtaca ttaagtacag aagagatagt
      781 aaatatagtt tcaacaaaga taggagaagt tgaagaggtt acagattttt caaaaatcta
      841 tgaaggtatt gttattgctc agattacaaa aaaagaagat catcctaatg cagacaaatt
      901 aggagtgtat atgataaata ttggggaaga aaaagaaatt caagtagtag ctggagacaa
      961 aagcctaaat gtaggagata aggtagcata tataactgtg ggacatattg ttccatacag
     1021 ctatgcgaca tcacagcctg tcaaaattaa agctgtaaat atgagaggca taacatcaaa
     1081 tggaatgtta tgttcacaaa aggagctaaa tatcggtcca gatgatacac atgtattggt
     1141 actagaaaaa gatgctcctg ttggagtacc ttttgcccag tattaccaat ttgatgatgt
     1201 tgttgtaggt atagagaata aagcacttac gaacagagga gatctctttg ggattcttgg
     1261 tttagcaaga gagatatctg cagctcaaaa tatcccattc aagagtccaa agtggtatct
     1321 aaaaacaaca tcagatatag atgtaactga aactctccct attaaagtcg ataatcaaat
     1381 gcaaaaccta tgtcctagat acacatgtat tgcaatcaaa gatgtccaaa ttaaagagtc
     1441 ccctgtatgg atgaaatcag tacttcttaa atcaggaata aaaccaataa ataatattgt
     1501 agatattaca aattatttat cagttatcac agctcagccc cttcatgcat ttgattatga
     1561 caaactagtg gagaatgatt taacaagaga tggtactgct catattacaa ttagacttgc
     1621 aaaaaatggt gaaagaattc acacattaga aggaaatatt gtagagctta gtgaggacaa
     1681 cctagttata gcagactcta ctaatcctat tgctattgca ggagtaatag ggggcattga
     1741 tacacaaata gacaataata caaagaacat tgttatcgag agtgctaatt ttaacaagta
     1801 cagtatcaga aaaacttcaa tggaacttgg tatatttact gaagctgtaa caagatttac
     1861 aagaggacaa gctccacagc tatctcttca aattttgctg aaagctgcag aacttgtaca
     1921 agaactggcc aatggggaag tagcatctaa tgttatagat atatatcctg aacctgctaa
     1981 accaaaaaaa ctgtctctat cagtatctag attaaattca catttaggaa ctgagctatc
     2041 aaaagaggaa attaagaata tacttttaaa tattgagtat gaaattataa aagaagatga
     2101 cgagtatctt accataatcc caccagtatt tagaacagat atctctattc ctgaagatat
     2161 atatgaggat gtaggaagaa tatatggata tgaaaacatt ttacctaaac tccctacaag
     2221 agaaataaat gcagtagtat tcaatgagaa aattgcatta aaagaaaaga taagaaatat
     2281 attaacatat agcggatgca atgagcttga tacttacagc tttactagca aagagagttt
     2341 gcaaaatgcg aaacaagatc caaatttcgc ataccatatt aaaaatgctc tgtctccaga
     2401 acttgaattt atgagaattt caatacttac ctcacttttg caaaaggcta aagaaaatat
     2461 tcaaaaaaat atttctccgc tgtgtatata tgagtttaat attcctcacc aaaaaggata
     2521 tatggataat tttcagcttc caaaagagga ttggcattta tcaatggtat tttcatcaaa
     2581 agaaaaaatt gtagatggaa gtccgtatta tcaaataaaa aggtatgcag aaaaaatatt
     2641 taattcagta ggagtagaga atatctcata tgatcttgtc tctttatcct ctcaaatgga
     2701 tttaccaata tggataaagt gtattatgcc taccttcaat cctaatcaat ctgcattaat
     2761 aaaataccac acagatggca cagaaagtat aataggaata gtaggagata tagattatga
     2821 ggtaaaaaac aattttaagc ttcctgattt tacagctgct ctagaaataa atcttgagga
     2881 attgataaag ataattaaac ctaaaacaat aagagttttt gattctaagt tcccttccat
     2941 atcacaagat atttgtttta cagttccaag ttctacaaag tactgcgata tagaaaatag
     3001 tgtactacag attataaaca caagagatag agtagctaca atagagtgta ttgatgttta
     3061 ccaaaaatca gaacactcta aaaatgttac tctaagaatt actattgagc atcaaaataa
     3121 aacattaagc gataaagaat ttagtaagat aaaagagaag atagaggaaa gaattaaaca
     3181 tctttaatta gtactaaggt gtttgtatag gaggaacaac tggaggagtt tcaggtcctt
     3241 gttcaacttt aaatg
//
LOCUS       DAOSAB010000057         3168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_20359_length_3168_cov_3.013813, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000057 DAOSAB010000000
VERSION     DAOSAB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20359_length_3168_cov_3.013813"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PK432_01580"
     CDS             complement(<1..483)
                     /locus_tag="PK432_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59647.1"
                     /translation="MEVLNALQLKSGKKAEYNRMGSITQPLQFLPRIEKNPEWTAWNM
                     DWLEWNGLKQIRRNARRLMKNYKLAKGVIDRTDYMVEEDNEMRDLVDTLMKEDPTALE
                     LKFYPIIPNVINVLTAEFAKRNTKITWQATDEYSYNEMLEQKRSQIEEVLFSQAEQKL
                     M"
     gene            complement(488..2689)
                     /locus_tag="PK432_01585"
     CDS             complement(488..2689)
                     /locus_tag="PK432_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59648.1"
                     /translation="MNSFFYTDIPTWDNGTWTITSFTSREEFRNFVLSIFKEPGKYNF
                     DETSFIFNEEAAKWNTQGFYTPAPVRSKDYITYWDEMKTRCRKGVIFKNKGATWYLTR
                     EYYMWLNFLPINNKEQRKFTFPDVRDAQYHLALYEILAELFYKHAAILKKRQIASSYF
                     HAAKLINQIWFEETPILKIGGSLKAYVLDTWRFLNEYRNFLDENTAWYRPMNPGKILD
                     WQQQIETKVPGSNRPTLKGLKGVMKGTSFEQDPTAGVGGPCTYFFHEEAGIAPDMMQT
                     YLYMKPALKSGLITTGTFIAAGSVGDLDQCEPLRKMIQTPEANDIFYVESDLLDDRGT
                     VGKSGLFIPEQWSMPPCVDKFGNSEVEKALQMLEEYFAKIKKDMSPEDYQLEVSQHPR
                     NIEEAFATRTVSIFPAHLVAAQKRRIQDKEYKIEYVDLSRKSDGTWLVEKSRKIPIMK
                     FPVEKNSEDKSGVIVVYEKPDSKAEWGTYYAAIDPVSQGKTVTSDSLCSIYIYKIPIE
                     VTRIDGEDVKTYIETDKIVASWCGRFDDINKTHERLENMIEWYNAWTIVENNVPGFLT
                     HMIKKRKQKYLVPKSQITFRKDLDNTLSHIEEYGWRNTGTVFKVHILPYLVDFCKEVV
                     EEITTDDGKVVKVVYGIERIPDEMAMVEMQQYREGLNVDRLIALGSLIAFAKVQEANR
                     GIRKRIDTTDKKHLEKSENLYKFTNSPFRHIGMGQSSLGKRPPRNPFKNIR"
     gene            complement(2686..2892)
                     /locus_tag="PK432_01590"
     CDS             complement(2686..2892)
                     /locus_tag="PK432_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59649.1"
                     /translation="MDELHNWLFHYNPYTESWNAFKRDHATEYFNGEYKNVIKSKAQK
                     TLESLIISHGGDIKKINKFVESCK"
     gene            complement(2897..3094)
                     /locus_tag="PK432_01595"
     CDS             complement(2897..3094)
                     /locus_tag="PK432_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59650.1"
                     /translation="MLDRLATYMEKTPISHGRDGNINSLVSAAKNFEGIRSSFKGAYK
                     DLLEEQQSRTRGGGELAYDQK"
BASE COUNT          883 a          666 c          521 g         1098 t
ORIGIN      
        1 cataagcttt tgttcagctt gtgagaaaag aacttcttca atttgacttc ttttttgttc
       61 taacatctca ttgtatgagt attcatctgt agcttgccag gtaatttttg tattcctttt
      121 ggcaaactca gctgtcaaaa cattaataac attagggata ataggataaa actttaactc
      181 aagtgctgta ggatcttctt tcatcagagt atctacaaga tctcgcattt cattgtcttc
      241 ttctaccatg tagtcagtac ggtctatcac acccttagcc agtttatagt tcttcattaa
      301 ccggcgcgca tttctgcgga tctgcttaag accattccac tctaaccaat ccatattcca
      361 ggcagtccat tcaggattct tttctattct aggtagaaat tgtaaaggtt gggttattga
      421 acccattctg ttgtactctg ccttttttcc agacttaagc tggagtgcgt ttaatacttc
      481 catagcatta tcttatattt ttaaatggat tccttggggg tctcttaccc aaagaacttt
      541 gacccatacc tatatgacgg aatgggctat tagtaaattt atacaaattt tctgactttt
      601 ccaagtgttt tttatctgta gtatcaattc tctttctgat acctctgttt gcttcctgaa
      661 ctttagcaaa agctatgaga gatccaagtg ctattaatct atccacgttg agaccttctc
      721 tgtactgctg catctcaacc atggccatct catccggaat tctttctata ccataaacta
      781 ctttaactac cttaccatca tctgtggtaa tttcttctac aacttcttta caaaagtcta
      841 ccaggtatgg taggatgtga accttgaaca cagtacctgt atttctccaa ccatactctt
      901 ctatatggga taaggtatta tcaagatctt tcctgaaggt gatttgactc ttaggtacaa
      961 gatacttctg ttttctcttc ttgatcatgt gggtgaggaa accaggcaca ttgttctcta
     1021 caattgtcca tgcattatac cactcgatca tattttccaa tctttcatgg gtcttgttga
     1081 tatcatcaaa gcggccgcac caagaagcta ctatcttatc agtttctatg taggtcttaa
     1141 catcttctcc atctatacgg gttacttcaa tagggatctt gtaaatgtag atagagcata
     1201 aagaatcaga tgttactgtt ttaccttgag atacagggtc aattgctgca taatatgtgc
     1261 cccattctgc tttagaatca ggtttttcat acacaactat aactccactt ttatcttctg
     1321 aattcttctc tactgggaac ttcatgattg ggatctttct actcttctca actagccaag
     1381 ttccatcaga ttttcttgaa agatctacat attcaatctt gtattcttta tcctgtatcc
     1441 ttcttttttg ggcagcaact aaatgtgccg ggaaaataga aacagttctt gttgcaaagg
     1501 cctcttcaat attgcggggg tgctgagata cctctaactg gtaatcctca ggactcatgt
     1561 ctttcttgat cttggcaaaa tattcttcta acatttgtag agctttctct acttcagagt
     1621 ttccaaactt gtctacacaa ggaggcatag accattgttc agggataaac aatccactct
     1681 tacctactgt acctctgtca tctaaaagat ctgactctac atagaatata tcattagctt
     1741 caggagtctg aatcattttt cttaaaggtt cacactgatc tagatcaccc acagaacctg
     1801 cagcaataaa agtaccggtt gtaatcaaac ctgatttcaa ggccggcttc atatacaaat
     1861 aagtctgcat catgtctgga gcaataccag cctcttcatg aaagaagtaa gtacatggtc
     1921 caccgacacc tgcagtagga tcttgctcaa aagatgtacc tttcattaca cctttcagac
     1981 ctttaagtgt aggtctgttt gatccgggca cctttgtctc aatttgctgt tgccagtcta
     2041 ggatcttacc tggattcata ggacggtacc aagcagtatt ctcatctaag aagtttctgt
     2101 actcatttaa gaatctccag gtatccaaaa cataggcctt aagacttcca cctatcttta
     2161 gaataggagt ctcttcaaac cagatctggt taataagttt tgcagcatga aaatatgagg
     2221 atgctatctg acgtttctta agaatagccg catgcttgta aaatagctca gccaagattt
     2281 catacaatgc caagtgatat tgagcatcgc gcacatccgg gaaggtgaac tttctctgct
     2341 ccttgttgtt gatcggtaag aagtttaacc acatgtaata ttcacgggta agataccaag
     2401 ttgctccttt attcttaaag ataacacctt tcctacatct agtcttcatt tcatcccaat
     2461 aggtaatgta atctttactt cttacaggtg caggtgtgta gaacccttga gtattccact
     2521 tggccgcttc ttcattaaat ataaatgatg tttcatcaaa gttatactta ccgggttctt
     2581 taaatataga aagaacaaag ttccggaact cttctctaga agtaaaacta gtaatagtcc
     2641 aagtaccatt atcccaagta ggtatatctg tataaaagaa agaattcatt tacaactttc
     2701 tacaaactta tttattttct taatatcccc accatgagaa attataagac tctctagagt
     2761 tttttgagct ttgctcttta ttacattttt gtactcacca ttgaaatact cagttgcatg
     2821 gtctctttta aaagcattcc aggattcagt gtagggattg taatgaaaca accaattgtg
     2881 taattcatcc atattattat ttttgatcat aagctaattc tccaccaccg cgggttcttg
     2941 attgttgttc ttctagtaaa tctttatatg cacctttgaa tgagctgcgg atcccttcaa
     3001 agttttttgc tgcactaact agagagttga tatttccatc ccgtccatgg gatataggag
     3061 ttttttccat ataagtagct aatcggtcta gcatagaact aatacctctg aaagcgcgta
     3121 ctgtaggagt ttcatacatt agagtgcact tatttaacgc tgttaaaa
//
LOCUS       DAOSAB010000058         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_20578_length_3144_cov_3.045970, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000058 DAOSAB010000000
VERSION     DAOSAB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20578_length_3144_cov_3.045970"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PK432_01600"
     CDS             <1..242
                     /locus_tag="PK432_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59651.1"
                     /translation="QHLEDFNEEIGEYKKLLSEKDRLINELREKIKNPYDFPTQTQKY
                     EEGFDVYTPKEREVVNSSKSKTFKEKLIDLIDTLF"
     gene            246..554
                     /locus_tag="PK432_01605"
     CDS             246..554
                     /locus_tag="PK432_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOK59652.1"
                     /translation="MPIKEFKTKVKNARIYKGMSQRTLGLALGLSDKTISSYESGRSY
                     PSLEILEKMADILDKPIEYFISSSKEIQINQDLENIDQLLKSITKEISDIKKILSKEE
                     "
     gene            complement(538..1155)
                     /locus_tag="PK432_01610"
     CDS             complement(538..1155)
                     /locus_tag="PK432_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59653.1"
                     /translation="MKRILLTLLTFTMLISPVFSQEQLNEASAEPVEEKVQIEDKETN
                     TTTSINKYFELELKRGTQNPITKTIPFTVYITPKINSSKTQILWNVPAVFDVERKHNE
                     FVSLAGGQTYSFSASLTPKKSGSYEVSVNVISWQTDSNKSNSADYKLVIDKSLTVQPV
                     DTMYTVYIILIIVGIIGVFVLLFFLIKKAANVLIKKAKSWLTPPY"
     gene            complement(1152..1568)
                     /locus_tag="PK432_01615"
     CDS             complement(1152..1568)
                     /locus_tag="PK432_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59654.1"
                     /translation="MDNQTVITIIILLLVALLFLIAYINSKKIPLKKKEEIFSKLSEL
                     ENQIKSPDDYARRDAVIKLDNLLAKSLNIRYGNDKQCGDNLKISKKLFDKKLYQQLWD
                     VHKLRNNIVHSDEDVPSVQAEEIYRIYKLGIKHILK"
     gene            complement(1561..>3144)
                     /locus_tag="PK432_01620"
     CDS             complement(1561..>3144)
                     /locus_tag="PK432_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOK59655.1"
                     /translation="DLVIDLEETPVYPDKPAVIKITYSKKLSGNTISLLSQIKNTQTK
                     EFSLTYPSSLGNISWCSATILEIKSVENKIKITTEIPITEYVKVTFGENIIYSYVIKK
                     NLLNAGDKMIIADIPLPVNNSFQHITVNSITPQPDKAYKDTDGNYLLQYSIAPLSSID
                     VEIQGYIFMNHSHYPEQLEPKFEKTQLWEINNISLINHVNKYIQSYGLNIPDTFSDIN
                     SLETSQEKELLYEAIYKYVIENLKPNISSAGSLMGGERLGGQQILLNQDIATDEDYAD
                     AIVSLYRYYNIPARFVIGYLSNISNYDSNGIFHFWAEYYNSEQNDWIIVEPFLEDYTK
                     TPLWKKEMKDHVALIYRSSNPYTPKLPFYVEGELTIEPVKENTNYKSNFDIELILKPY
                     RLTDPFLLGYISLTNTGTTVIDKFEIVNSKPDLSKYIDYIENNSSIIILPSQTYDIKF
                     NIPAEQIPDEIFVSMNAFAGTDKIEGVQTKKEMEVIKSYSDLDILSKLIPILIYFLLS
                     LSVYFILKKVKYKKVKRNG"
BASE COUNT         1028 a          425 c          486 g         1205 t
ORIGIN      
        1 aacagcattt agaagatttt aatgaagaaa taggggagta caaaaaattg ttaagtgaaa
       61 aagacaggct tattaatgag cttagagaaa aaattaaaaa tccgtatgat tttcctactc
      121 aaacacaaaa gtatgaagag ggctttgatg tatatacacc aaaagaaaga gaagtagtga
      181 atagtagtaa aagtaaaact tttaaagaaa aattaataga tttaattgat acattattct
      241 agtttatgcc tattaaagaa tttaaaacaa aagttaagaa tgcaagaata tataaaggta
      301 tgtcacaaag aactctcggt cttgcattag gattatcaga taaaacgata agttcatatg
      361 aatcagggag atcgtatcct agtctagaga tactggaaaa gatggctgat attcttgata
      421 aaccaattga gtattttatt tcttcttcca aagaaattca gattaatcaa gatttagaaa
      481 atattgatca gcttcttaaa tctattacaa aagagatatc tgatattaaa aaaattctta
      541 gtaaggagga gtaagccagc tcttagcttt ttttattaat acattcgctg cctttttaat
      601 aaggaagaat agcagtacaa atacaccaat aatcccgaca ataatcagaa ttatgtatac
      661 agtatacatt gtatcaacag gctgtactgt taatgattta tcaataacca gcttataatc
      721 agcactattt gatttgtttg aatcagtttg ccatgatatt acatttacag atacctcata
      781 tgatccagat tttttaggtg ttagagatgc tgaaaatgaa tatgtttgtc ctcctgcaag
      841 agatacgaat tcattgtgtt ttctttctac atcaaataca gctggaacat tccatagaat
      901 ttgagtcttt gaagaattaa ttttaggtgt tatatacact gtaaaaggaa ttgtttttgt
      961 tattggattt tgtgtgcctc ttttaagttc aagttcaaaa tatttattta tacttgttgt
     1021 tgtatttgtt tctttatctt ctatctgaac tttttcttct actggttcag cagaagcctc
     1081 atttaactgc tcttgagaaa agacaggaga gattagcatt gtaaaagtaa gtagtgtaag
     1141 taatattctt ttcattttaa tatgtgtttt attcccaatt tatatattct gtaaatttct
     1201 tccgcttgaa ctgaaggaac atcctcatca ctgtgtacaa tattatttct tagtttatga
     1261 acatcccaca gttgttgata aagcttttta tcaaatagct ttttagagat ttttaaatta
     1321 tctccacact gtttatcatt accataccta atatttaaag actttgctag tagattatct
     1381 aattttatta cagcatctct tcttgcataa tcatctgggc ttttaatttg attttcaagt
     1441 tcactcaatt tagaaaaaat ctcctccttt ttcttaagag ggattttttt actgtttata
     1501 tatgcaatta agaaaagaag agcaacaagc agaagaatta ttattgttat gactgtttgg
     1561 ttatccattt ctttttacct ttttatattt tacctttttt aagataaaat atacagaaag
     1621 ggataggagg aaatatataa gaatgggtat aagcttagaa agaatatcta aatcagagta
     1681 acttttaatt acttccattt ctttcttagt ttgtactcct tctattttat ctgttcctgc
     1741 aaatgcattc attgatacaa atatttcatc tgggatttgc tctgctggaa tgttaaattt
     1801 aatatcatat gtttgactgg ggagaataat tatactgctg ttattttcta tataatcaat
     1861 atattttgat aaatcaggtt ttgaattaac aatctcaaat ttatctatta ctgttgtccc
     1921 tgtatttgtt aaactaatat atccaagaag aaaaggatca gtcagtctat atggtttaag
     1981 tattagttct atatcaaaat tgcttttgta attagtgttt tcttttacag gttctatggt
     2041 tagctctcct tcaacataga agggaagttt tggagtgtaa ggattggaac ttctatagat
     2101 taaagcaaca tggtctttca tttctttttt ccataaaggg gtttttgtat aatcttcaag
     2161 aaatggttct acaataatcc aatcattttg ttcactgtta tagtattcag cccagaaatg
     2221 gaatatacca tttgagtcat aatttgaaat attgcttaaa tatccaatta caaatctagc
     2281 tgggatatta taatatctat acaaggaaac aattgcatct gcataatctt catctgttgc
     2341 tatatcttga tttaaaagta tctgttgtcc acctaatctt tcacccccca taagagaacc
     2401 tgctgatgag atattgggct taagattttc aattacatat ttatatattg cttcatacaa
     2461 aagctctttt tcttggcttg tttctagcga gtttatatca gaaaaagtat ctggaatatt
     2521 aagaccgtat gattgaatat atttattcac atggttaata agtgagatat tgtttatctc
     2581 ccataattgt gtcttttcaa atttaggttc taattgctct gggtagtgtg aatgattcat
     2641 aaagatatat ccttgtattt caacatcaat actactaaga ggtgcaatac tatattgaag
     2701 aaggtaattt ccatcagtat ctttgtatgc tttatcaggt tgaggggtta tactatttac
     2761 tgtaatatgc tgaaaactat tgtttactgg tagggggatg tctgcaatta tcattttatc
     2821 ccctgcattt agtaagttct ttttaataac atatgaatat attatatttt ctccaaatgt
     2881 tacttttaca tactctgtaa taggtatttc tgtagtaatt ttaatcttgt tttctacact
     2941 tttaatttca agaatagtag ctgaacacca tgagatgttt cctagtgaag aagggtatgt
     3001 taggctaaat tcttttgttt gtgtattttt aatttgtgat aatagagata ttgtattccc
     3061 agatagtttt ttggagtatg ttatttttat aacagcaggt ttatctggat atacaggggt
     3121 ttcttctaaa tcaattacaa ggtc
//
LOCUS       DAOSAB010000059         3046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_21466_length_3046_cov_2.795052, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000059 DAOSAB010000000
VERSION     DAOSAB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21466_length_3046_cov_2.795052"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..2144)
                     /locus_tag="PK432_01625"
     CDS             complement(36..2144)
                     /locus_tag="PK432_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOK59656.1"
                     /translation="MKDEILFPKSIAVVGVSSDKSKIGRVIYDNARNGGYQGKIFPVN
                     PKYDNIDGEKCYPTVNDIGDPIDMVCISLPYQLVEGIVDDCILKKVKSVIIISAGFKE
                     TGKQGKELEERIAQKLKSAGIRLIGPNTLGIINNEINLNLSFARKNPGAGTTAFISQS
                     GAFCTAILDMALRDNIGFSKVISIGNKADINENELIEYLQRDKSTDSIAIYLEEFSDG
                     KDFVEIAQKSEKPMIIIAPGSSQQGKAAISSHTGSLASSYDTTVAAIQKGNLIKVESS
                     EEMFDAIKVLSMKKMPKGRGVAIVTNGGGPGIMATDCVERFGLELAQIGEKTKEKLSK
                     FLPIQSNINNPVDILGDALLDRYEYAIKTLLDDISVNTLVVILTPQLVTDIVGVAKAI
                     ADIQKQTSKPIFSCFLGGSDVEDGNKILRAYGMYVSNNIEDTVRLISMITNYELARTN
                     KSIRRTSDLLSKGRYKKAVLLELKEDEATVLSDDISEKIAKEVGISLPKQIITANINE
                     AIQFASKNFPVVIKAVSKDLTHKTDVRGVFVDIRTISELQEKFNILSSNIEKVTGRKY
                     PEILVQEMIEPKLEFFIGANREGGADIYEKEGRGFGHLIAIGQGGVYTEVYQDIQHIL
                     VPESIKNITEKLNATKVVKIIDGYRGKPALPKQKLIEMIDKVQRLLVTYPQIFSIDMN
                     PVILTENRVVAVDLKIYIKS"
     gene            complement(2147..>2396)
                     /locus_tag="PK432_01630"
     CDS             complement(2147..>2396)
                     /locus_tag="PK432_01630"
                     /inference="COORDINATES: protein motif:HMM:NF023785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59657.1"
                     /translation="TYSPENSLDFLKSKMINVAPTDDISKALKRVTNKEKMYTGVIYQ
                     NKKIKDYYSNLKNRKDKKSELVNEVKNYSISSILSDLE"
     assembly_gap    2397..2406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2407..3008)
                     /locus_tag="PK432_01635"
     CDS             complement(<2407..3008)
                     /locus_tag="PK432_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK59658.1"
                     /translation="MKVSLQDYNTDIKNTWCPGCGNFAIHMALKKALVDLGKHPSEVV
                     MVFDIGCNGNGADKIEGFRVKGLHGRAIPLAVGCHLANRKMTVIADAGDGGVLHEGID
                     HLIHAVRSNYDITLLIHNNESFSLTTGQITATTKKNKPMYAFPDGKTEGDINIGELVL
                     SLNPSFYARGFSGDPNGLSEIIKAGIKHKGFSVIEIVQICP"
BASE COUNT          874 a          594 c          407 g         1161 t
ORIGIN      
        1 aagagaggat tataaagagg aaatacttaa aatggttaac tcttaatata tatctttaag
       61 tcaactgcca ctactctatt ttctgttagt attacagggt tcatatcaat actgaaaatt
      121 tggggatatg taacaagcag tctttgtact ttgtctatca tttctataag cttttgttta
      181 ggaagtgcag gttttcctct atacccatca attattttta caacctttgt agcatttaat
      241 ttttctgtta tattttttat actctcagga actaatatgt gctgaatatc ttgataaacc
      301 tctgtataca ctccgccttg tccgattgca attaagtgac caaagcctct cccttccttt
      361 tcataaatat ctgcaccacc ttctctgttt gcaccaataa aaaactcaag tttaggttca
      421 atcatctctt gtactaaaat ctcaggatat tttcttcctg ttactttctc aatattgcta
      481 gataaaatat taaatttctc ctgcagctca gatattgttc taatgtctac aaatactcct
      541 ctaacatctg ttttatgagt taaatctttt gatactgctt taataacaac aggaaagttt
      601 ttacttgcaa actgtatagc ttcatttata ttggcagtaa ttatctgctt aggaagtgat
      661 attcctacct cttttgcaat tttttcacta atatcatcag aaagaacagt agcttcatcc
      721 tcttttagtt caagcagtac tgccttttta tatctgcctt tagataagag atcagatgtt
      781 cttctaatac ttttatttgt tcttgcaagt tcatagtttg taatcatgct tataagtctt
      841 actgtgtcct caatattgtt tgatacatac attccatacg ctctaagtat tttattacca
      901 tcttcaacat cactacctcc taaaaagcat gagaatattg gttttgaagt ttgtttctgg
      961 atatcagcaa tagcctttgc aaccccaact atatctgtaa caagctgtgg cgttaatatt
     1021 acaacaaggg tgttaacaga aatatcatca agaagtgttt taattgcata ttcgtatcta
     1081 tcaagaagtg catcaccaag aatatctaca ggattattaa tatttgattg tattgggaga
     1141 aatttagaaa gtttttcttt tgttttctcc cctatttgtg ctaattcaag accaaacctt
     1201 tctacacagt ctgttgccat tattcctgga ccacctccat tagttacaat tgcaacacct
     1261 cttcccttag gcatcttttt catagaaagc acttttattg catcaaacat ctcttcagaa
     1321 ctttctacct ttattaaatt tcccttttga attgcagcaa cagttgtatc gtatgagctt
     1381 gcaagagaac ctgtatgtga tgatattgca gctttacctt gttgtgagct acctggtgca
     1441 attattatca ttggtttttc tgacttttgt gcaatttcaa caaaatcttt accatcacta
     1501 aactcttcaa gatatattgc aatagagtct gtacttttat ctctttgcaa atactctatt
     1561 aactcatttt catttatatc tgctttgttt ccaatggata ttactttgga aaacccaata
     1621 ttatcccgca gtgccatatc taaaatagca gtacagaaag ctcctgactg agagataaag
     1681 gcagttgtcc cagcgcctgg gttttttctt gcaaatgaaa ggtttagatt aatctcatta
     1741 ttaataattc caagagtatt aggaccaatc aatcttatac cagcactctt tagcttttga
     1801 gctattctct cttcaagctc ctttccctgt tttcctgttt ctttaaaacc agcagaaatt
     1861 attattacac tcttaacttt ttttaaaata cagtcatcta ctataccttc tactaattga
     1921 taagggagcg agatacatac catatcaata ggatcaccta tgtcatttac tgttggatag
     1981 cacttctctc catcaatatt gtcatattta ggattaacag gaaatatttt tccttgatat
     2041 cctccgtttc gtgcattgtc gtaaatgact cttcctatct tacttttatc acttgataca
     2101 ccaactactg ctatgctttt aggaaatagt atttcatctt tcatttttat tcaaggtcag
     2161 ataatattga agatatactg taatttttta cctcattgac tagttcactc tttttatctt
     2221 ttctgttttt aagattggag taatagtctt ttatcttttt gttttgatat ataactccag
     2281 tatacatttt ttctttgttt gttactcttt ttaaagcttt agatatatca tctgttggtg
     2341 ctacattaat catcttgctt ttaagaaagt ctaaactgtt ctctggtgaa tatgttnnnn
     2401 nnnnnnggac atatctgaac tatttcaata acactaaagc ctttatgctt tattcctgcc
     2461 tttattattt cgctaagccc gtttggatct ccagaaaaac ctcttgcata aaaagaggga
     2521 ttaagagaaa gaacaagctc tccgatatta atatctcctt ctgttttacc atcaggaaat
     2581 gcatacattg gtttattctt cttcgttgtt gcagttatct gccctgttgt aagagaaaag
     2641 ctctcattgt tatgaataag taaagtgata tcatagttag accttacagc atgaattaaa
     2701 tggtctattc cctcgtgtaa aactcctcca tctccagcat ctgcaattac tgtcattttt
     2761 ctatttgcga gatgacaacc aacagcaaga ggtattgctc tcccatgaag accctttact
     2821 ctgaaccctt caattttatc tgctccattc ccattacacc ctatatcaaa caccattact
     2881 acttcacttg gatgctttcc taaatcaact aaagcttttt taagtgccat atgaattgca
     2941 aagtttccgc atccagggca ccatgtattt ttaatatctg tattgtaatc ttgtaaactt
     3001 accttcattg ttttaaatat tctaaaatat catctacaaa aaatgg
//
LOCUS       DAOSAB010000060         2990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_21956_length_2990_cov_2.931516, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000060 DAOSAB010000000
VERSION     DAOSAB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21956_length_2990_cov_2.931516"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="nrdD"
                     /locus_tag="PK432_01640"
     CDS             complement(<1..924)
                     /gene="nrdD"
                     /locus_tag="PK432_01640"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOK59659.1"
                     /translation="MTKKKQNSIPFKVRKRDGRLVEFDQERITSSIFKAAESVGGKDR
                     ERAKKVSDEVVKRLLEKYSSDEEITTEEIFSEVKETLKDMGHGKTAMALDLFVKLKNK
                     VKNIKSLIDADELVRGYIDEADWRVKENSNMTYSWQGLNNHISSTVQANYWLYSIYPK
                     SISNAHVNADFHIHDLGMLATYCCGWDLKDLLLRGFTGVPGKISCAPPKHFSAALGQC
                     VNFIYTLQHEAAGAQAFSSFDTLLAPFIRYDKLSYKEVKQKMQEFLFNMNVPTRVGCQ
                     CVSQDTEILTPNGWKSYKDIEKGVVIKTFNLN"
     gene            complement(936..1379)
                     /gene="nrdR"
                     /locus_tag="PK432_01645"
     CDS             complement(936..1379)
                     /gene="nrdR"
                     /locus_tag="PK432_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOK59660.1"
                     /translation="MRCPYCGSSASKVVDKRDNSEDHSTRRRRQCLRCGRRYTTYERI
                     EKVEVVVIKKDGSFENFDCEKLKKSIRKAIDKEKISEEEIEEFCERVTRKAITSPTPI
                     TTVEIGNMVLEWLKTLDPLAYMRFASVYKDFETINDLKKEIENLT"
     gene            1603..2112
                     /locus_tag="PK432_01650"
     CDS             1603..2112
                     /locus_tag="PK432_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOK59661.1"
                     /translation="MIVTIQEFKKIRENELKDKKIVCTSGYFDPIHPGHVSCILESKK
                     FGDVLVVIIDGDSRCINKKGKAFMPAKDRADIVNAIKDVDYVIIHENPQATNCSEVLA
                     VVKPDVFTKGGDRDDKDRNNPNSGLKVEADLIESYGGRIEYGVGKEKVWSSSNYLQEW
                     VEFVNSTKN"
     gene            complement(2109..>2990)
                     /locus_tag="PK432_01655"
     CDS             complement(2109..>2990)
                     /locus_tag="PK432_01655"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK59662.1"
                     /translation="YASRYKLSNIVFGKVLGGEITGAHKVCNFPGLQDISGIDLGMRM
                     YEHAKSLGGQYIAEGIKDIKKENNMFVLISETGKEYLSKTVIIATGTNRNKLNLPNEE
                     YYLGKGLSYCCTCDGMFFKNKTVAVVGGSSAATMAACMLSDIAKKVYIIYRGAQLRGD
                     KTWIEEAVNKKNVEVIYTTVVTALEGKEKLEKIKLSKPYKGSEYLDVDGLFVEIGSQP
                     YIDIPNKLGVELDEKGYIKVLGNQSTNVEGVWAAGDCTNALDNFKQVITASAQGAVAS
                     NSIYLYLAGGEKTPSFN"
BASE COUNT          956 a          594 c          416 g         1024 t
ORIGIN      
        1 atttaagtta aaagtcttta tcacaactcc cttttctata tctttgtacg atttccaacc
       61 atttggtgtg agtatttcag tatcttgtga tacacactga catcctactc ttgtaggaac
      121 attcatatta aaaagaaact cctgcatctt ctgttttacc tctttgtatg acaacttgtc
      181 atatcttata aacggagcaa gaagagtatc aaaactagaa aaagcttgag ctccagcagc
      241 ctcatgctga agggtatata tgaagttcac acactgaccc aatgctgcac taaaatgctt
      301 tggaggagca catgatatct ttccaggaac acctgtaaaa cccctaagaa ggagatcttt
      361 taaatcccat ccacagcaat atgtagcaag cataccaaga tcatgaatat gaaaatcagc
      421 attaacatga gcattggaaa tagatttagg atatattgag tatagccagt aatttgcctg
      481 tacagtagaa gaaatatgat tattaagccc ctgccatgaa tatgtcatat tactattctc
      541 cttaactctc caatcagcct catctatata ccctcttaca agctcatctg catctattaa
      601 tgatttaata ttttttactt tgttcttaag cttaacaaac aaatcaagag ccatagcggt
      661 cttaccatgc cccatatctt ttaatgtttc ctttacctca gaaaatatct cttcagtagt
      721 aatctcttca tcacttgagt acttttcaag aagtctttta acaacctcgt cagacacttt
      781 tttagctctc tccctatcct ttcctccaac actttcggca gctttaaata tagaagatgt
      841 aattctttcc tgatcaaact ctactaatct tccatctctt tttcttactt taaaaggaat
      901 actattctgt ttcttttttg tcatggcagt tttaactaag ttaagttctc aatttctttt
      961 tttaaatcat taatagtttc aaagtcttta tatactgatg caaatctcat atacgcaaga
     1021 gggtcaagag tcttaagcca ttctaaaacc atattcccta tctcaactgt agtaattgga
     1081 gtaggactgg taattgcttt tcttgttacc ctctcacaaa actcttcaat ttcctcttca
     1141 cttatcttct ccttatcaat tgcttttcta atacttttct ttaatttttc acaatcgaaa
     1201 ttctcaaaag agccatcttt tttaattaca acaacctcaa ccttttcaat tctttcatat
     1261 gtagtgtatc ttcttccgca tctcaagcac tgccgccttc ttctagttga gtgatcctca
     1321 ctgttatctc ttttatctac tacttttgat gcactgcttc cacagtatgg acacctcata
     1381 tttacaacct gttgtattta gtatattagg ctaccccaac tcattgtatc agtcaaagac
     1441 ctataacttt gtcaaatgca gttattgcct acaatatgtg gtatttatgt aaagtaaaaa
     1501 taactacagg ttgtattaca catcttacaa gttaaaatat attcccattg caatataata
     1561 atttaactgg ttaaatatta aataaattaa aaaaattaaa acatgatagt aactatacaa
     1621 gagtttaaga agataagaga aaacgagtta aaagataaaa agatagtatg tacatcagga
     1681 tattttgatc caattcaccc aggacatgta tcatgcattc tagaatcaaa aaaatttggt
     1741 gatgtattgg ttgtaattat tgatggtgat agcagatgta ttaataaaaa gggaaaagca
     1801 tttatgcctg caaaagacag agctgacatt gttaatgcta ttaaagatgt agattatgta
     1861 attatccatg aaaaccctca agcaacaaac tgttcagaag tactggcagt agtaaaacca
     1921 gatgtcttta ctaaaggagg agatagagat gacaaagata gaaataaccc caatagtgga
     1981 ttaaaagtag aagcagacct tatagaatcc tatggcggaa gaatagagta tggagtagga
     2041 aaagagaaag tatggtcaag ctctaactac cttcaagagt gggtagagtt tgtaaattca
     2101 actaaaaact aattaaaaga gggtgttttt tcaccacctg ctaagtataa gtatatggag
     2161 ttagaagcaa cagccccttg tgcagatgca gttataactt gtttaaagtt atcaagagca
     2221 tttgtacagt cccctgcagc ccatacgccc tcaacatttg tagattgatt gcccaacacc
     2281 ttaatatatc ctttttcatc tagttcaaca ccaagcttat ttgggatatc tatatatggc
     2341 tgtgacccta tctcaacaaa aagaccgtca acatctaagt actctgagcc tttgtatggc
     2401 tttgagagtt taattttttc taatttctcc tttccctcta gtgcagtaac aactgttgta
     2461 tatatcactt ctacattctt tttgtttaca gcttcttcta tccaagtttt atcacccctt
     2521 agttgtgctc ctctataaat tatatatacc tttttagcaa tatcagaaag catacatgca
     2581 gccattgttg cagcactact tccacctaca actgcaactg ttttattttt aaaaaacatt
     2641 ccatcacatg tacaacaata tgaaagacct tttccaagat aatactcttc attaggtaaa
     2701 ttaagtttgt ttctgtttgt accagttgca ataatgactg ttttagagag atattccttt
     2761 cctgtttcag atattaaaac aaacatatta ttctcttttt taatgtcttt aatgccttct
     2821 gcaatgtact gccctcctaa actctttgca tgttcataca tcctcattcc aagatctatt
     2881 cctgaaatat cttgcagacc tgggaaattg cataccttat gtgcaccagt tatttctcct
     2941 ccaagaacct tgccaaaaac aatattactt aatttatatc tgcttgcata
//
LOCUS       DAOSAB010000061         2985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_22000_length_2985_cov_3.155631, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000061 DAOSAB010000000
VERSION     DAOSAB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22000_length_2985_cov_3.155631"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PK432_01660"
     CDS             <1..660
                     /locus_tag="PK432_01660"
                     /inference="COORDINATES: protein motif:HMM:NF025881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HOK59663.1"
                     /translation="HFFDNIDNEEKAYWLGFLYADGCVQNKKTGQKLISLAVKDKEVI
                     DKFIKSIDGNFQTKTYKDVHVVHLTSVIMFNALIKYGCIPRKSLVLKFPVLRKDLVHH
                     FIRGYFDGDGTVYITNPKNYNKTNTVYSSIGIGICGTQEFLAEVSREVSINQAKKDKR
                     KENNIWYLSFSGSKKAKAFYEYLYKDATICLTRKKDKFEQYFKERGSTTTIDHPNRMK
                     V"
     gene            694..1638
                     /locus_tag="PK432_01665"
     CDS             694..1638
                     /locus_tag="PK432_01665"
                     /inference="COORDINATES: protein motif:HMM:NF021484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HOK59664.1"
                     /translation="MIEINQYIDKDLLPPGPQLPETYPELKLEPKKGTKYYYDKLMQG
                     KKAGNCPNLNAMLAASAMGIGMGDGKGKGKTVKVRGKGGGQMEVQLPDHSTWEEFDNL
                     DEATKKLINSQTAHILKEVSEQVQKSRGTVPSEMAEILERITKLEPPKFNWKGYLRRF
                     VGGSIKTFTKMSKSKPNFRFLDNPGLKHKERRKILVAIDTSGSVSTNELKEFLGEINH
                     ISRTGTEVIIVQCDAAISNIGKFNPKEDFKVHGRGGTSFHPVTDYYDANFRKFNCLIY
                     LTDGEASAPHKCRGPVLWVHSSKSNINEDLQGLKIKLN"
     gene            1680..2432
                     /locus_tag="PK432_01670"
     CDS             1680..2432
                     /locus_tag="PK432_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD1 domain-containing DNA-binding protein"
                     /protein_id="HOK59665.1"
                     /translation="MKNILDRLKFKDLINKPGVYILQFKKYSYIGSSKNLYVRLKEHH
                     NSLKKGNHYNLFLQRLYNKYGEIAFKYDILEYCDNYIEREGFYIRSLSPKINVEQDPT
                     CKVKSQSTKDKIGKAMLGKLAGSKNPAARIVYQYNLAGDFIKEFSCATEAAKAVNGND
                     TAIGVAANGKIKSSAGYLWSRVKVDKITPLTSRNRKPYKYNQILVIDKSGNITSWQNI
                     NNLAKHLNISIQAVHQAIKKQRLCKGLKIKLN"
     gene            2434..>2985
                     /locus_tag="PK432_01675"
     CDS             2434..>2985
                     /locus_tag="PK432_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59666.1"
                     /translation="MSQKINLNAEELKGFLTYIHNNNQLLISRHLPAQTVNIEGEAGG
                     GKTSTVLQLANELGMEVIRKNLAELEDVSDLVGYPCKEHEMLSKDKVTKKWVVEGSIP
                     QYITAGYAPTGEKRMTHAAPEWIQGKDKPLILLLDDYSRASEKFIQATMTLLENQSYN
                     NWKLPAGSFVLLTSNPDNGEYSVS"
BASE COUNT         1143 a          407 c          573 g          862 t
ORIGIN      
        1 cacttctttg ataatattga taatgaagaa aaagcttatt ggttaggctt cttatatgca
       61 gatggatgtg tgcaaaataa gaaaaccggt caaaagctta tatcattagc agtaaaagat
      121 aaagaagtaa ttgataaatt tattaagtct atagatggaa attttcaaac aaaaacttat
      181 aaagatgtgc atgttgtgca cttaactagt gtaataatgt ttaatgcttt aataaagtac
      241 ggatgtatac ctagaaaatc attagtttta aaatttccag tgttaagaaa agatcttgta
      301 catcacttta tcagaggtta ctttgatggt gatggtactg tatatataac taatcctaag
      361 aattataata agacaaatac tgtctatagt tcaattggta taggtatatg cggtactcaa
      421 gagtttttag cagaagtaag tagagaagta tctataaatc aagctaaaaa agacaaaaga
      481 aaagaaaaca atatttggta cttatctttc agtggtagta agaaagcaaa agctttttat
      541 gagtatcttt ataaagatgc tactatatgt ctcacaagaa agaaagataa gtttgaacaa
      601 tactttaaag aaagaggttc aacgactaca atagaccatc ctaataggat gaaggtatag
      661 tctgatctta taggaaacta taagttaaca caaatgatag agatcaatca gtatattgat
      721 aaggatttgc taccaccagg accacaacta ccagagactt atccggaact aaagcttgaa
      781 ccaaagaaag gtaccaagta ttattatgat aaattaatgc agggtaagaa agctggcaat
      841 tgtcctaatc ttaatgctat gcttgcagct tctgctatgg gtataggaat gggagatggt
      901 aaaggtaagg gaaagacagt aaaggtaaga ggtaaaggtg gaggacagat ggaagttcaa
      961 ttaccggatc acagtacttg ggaagagttt gataacttag atgaagctac aaagaagctt
     1021 atcaatagtc agacagctca catacttaaa gaggtatctg agcaggttca aaagagccgt
     1081 ggcacggttc ctagtgagat ggcagagatc ctagaaagga tcactaagct agaaccacct
     1141 aagtttaact ggaagggtta tctaagacgc tttgtaggag gtagtataaa aaccttcacc
     1201 aaaatgtcca agtcaaaacc taacttcaga ttcttagata atcctggact taagcacaaa
     1261 gaaagaagaa aaatcctagt agcaatagat acttcaggat ccgtaagtac aaatgaactt
     1321 aaagaattcc taggtgagat taatcatatc tctagaacag gtaccgaagt aataatagtt
     1381 cagtgtgatg ctgcaatatc taacataggt aagttcaatc ctaaggaaga ctttaaagtc
     1441 catggtagag gaggcacaag cttccatcca gtaacggatt attatgatgc aaatttcaga
     1501 aagtttaatt gcttaatcta tctaacagat ggagaagcat cagctcctca caaatgccgg
     1561 ggcccggtat tatgggtaca ttcatcaaag tctaacatta atgaagattt gcagggttta
     1621 aagatcaagt tgaattaagt ttttaaggct agttcagtta taagttgtat atttgtaaca
     1681 tgaaaaatat tttagataga cttaaattta aagacttgat taataaacct ggagtttaca
     1741 tattgcaatt taaaaaatat tcttatatag gaagtagtaa aaacttatat gtaagattaa
     1801 aagaacatca taattcttta aaaaaaggaa atcactataa tttatttcta caaagattgt
     1861 ataataagta tggtgaaata gcttttaagt atgatattct tgaatattgt gataattaca
     1921 tagaaagaga aggcttctat attagaagtc tttctcctaa gataaatgtt gaacaagatc
     1981 ctacttgtaa agtaaaatct caaagtacta aagataaaat tggtaaagca atgcttggta
     2041 aattagcagg atcaaaaaat cctgctgcta gaattgtata tcagtataac ttagctggtg
     2101 attttattaa agaattttct tgcgcaacag aggctgctaa agcagtaaac ggaaatgata
     2161 cagctatagg cgtagctgct aatggaaaaa ttaaatcatc tgctggatat ttgtggagta
     2221 gggttaaggt tgataaaata actcctttga cttcaagaaa tagaaaacca tataagtaca
     2281 atcaaatatt agtgattgat aaaagtggaa atattacttc ttggcaaaac attaataatt
     2341 tagctaaaca tttaaacata tctattcagg cagtacatca agcaattaaa aaacagagac
     2401 tttgtaaggg tcttaaaatc aaattaaatt aatatgtcac aaaagatcaa tttaaacgca
     2461 gaagaactaa aagggttctt aacttatatt cataacaata atcaactgct aataagcaga
     2521 caccttcctg cacaaactgt aaacattgaa ggtgaagcag gaggaggaaa gacttcaaca
     2581 gtgttacaac ttgctaacga gttgggtatg gaagttataa ggaaaaattt agctgagtta
     2641 gaagacgtat ctgacttagt aggttatcct tgtaaagaac atgagatgct cagtaaagat
     2701 aaagttacta agaaatgggt agtggaggga tcgattcctc aatacattac tgcaggttat
     2761 gcaccaactg gtgagaagcg catgactcat gctgctccag aatggatcca aggtaaagac
     2821 aaaccattga tcttgttatt ggatgattat agtagagctt ctgagaagtt cattcaagca
     2881 actatgacat tgttggaaaa ccaaagttac aataactgga agttaccggc tggttcattt
     2941 gtgctattaa cttctaatcc agataatggt gaatatagtg tatct
//
LOCUS       DAOSAB010000062         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_22150_length_2969_cov_2.706589, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000062 DAOSAB010000000
VERSION     DAOSAB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22150_length_2969_cov_2.706589"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PK432_01680"
     CDS             complement(<1..927)
                     /locus_tag="PK432_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOK59667.1"
                     /translation="MKLESKKIKRIKADPYLLIPSILISFIGIVTLLSTTIDSNGQFG
                     DVSIVQKQVIFLLIGFIIYFLISLFDASLFKYTQIIIPIYILTLILLICVYLFGPVIN
                     NVQRWLVIGGVQLQPSEIAKVTVILTTACIFSFKGKYNQWILFLISFLAVLPIFLLVY
                     FEPAGSMSLLILLIWFLVAFTGLSDQLRNSIALFIAALVAISIFLVSFSGNWLYILIG
                     VAGAIIAIFAFYFRDSWRIIVSVSFCVGVLLGLVSMFFYNDVLKDYQKKRIEAFLNPT
                     QNTQDISFNVNQARIAIGSGQILGKGFGNGTQS"
     gene            complement(982..2163)
                     /gene="ftsZ"
                     /locus_tag="PK432_01685"
     CDS             complement(982..2163)
                     /gene="ftsZ"
                     /locus_tag="PK432_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOK59668.1"
                     /translation="MPLITTNVDPVAKIKVIGVGGAGCNAINTMMTDYDIPGVEFMAF
                     NTDAQTLKLSKAPVTLQLGENLTKGLGVGGNHELGAQAAEESLDQIQELLEGADMVFI
                     TAGMGGGTGTGAAPIIAGVAKNMGALTVAVVTKPFDFEGKRRMEVAEEGLNNLRDKVD
                     TLIIVPNQRLLEIATDNISFIDAMKMVDSVLAEGVNSISMLISQTGYINADFNDAKSV
                     MQNAGTALMGIGRASGEKRAELAARAATTSPLLDMSIEGATGVLYNIVGADLTLPEIS
                     LVSELIREAVDPSANIKFGAQIDPSLGDEIVITIVATGFDEKKQVYTKKQKGEEDETD
                     IFGLEGVTDEIKSFEEEKTSEVNVEKEEESFIDTIPQPDDFEDPLDKPAFMRKIFKKK
                     E"
     gene            complement(2199..>2969)
                     /locus_tag="PK432_01690"
     CDS             complement(2199..>2969)
                     /locus_tag="PK432_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HOK59669.1"
                     /translation="LGLNVYDVVFTGWASSLSVLTDTEKELGVTMLDIGAGTTSISIF
                     DEGSIIYSGVIPLGGINITSDLAIGLQVSLEEAEKIKIRMGDLLDNKAKAKKSSSDST
                     PALLRKLDEEEPKTKKGDEIDLSVLDITGKKVSKELLVKIVEARCEEIFDMAKEKVGK
                     AGFKMAMPAGIVLTGGTSLLRDLPKFTQGVFGVAARVGYPSGLTGMIEEISDPSFACV
                     QGLIKYALEEDGESETMKVKGGGNLKDKIVEWFKSLMP"
BASE COUNT          970 a          662 c          379 g          958 t
ORIGIN      
        1 actttgagta ccatttccaa agcctttccc taatatctgt ccagacccta ttgcaattct
       61 tgcttgatta acattaaaac taatatcctg agtattttga gtaggattta aaaaggcctc
      121 aattctcttt ttttggtaat cttttaatac atcgttatag aaaaacattg atacaagacc
      181 taatagcacc ccgacacaaa agcttactga tacaatgatt ctccagctgt ctctaaaata
      241 gaatgcaaaa atcgcaataa ttgcccctgc tacgccaatt aagatatata gccaattccc
      301 actaaaacta actaagaata ttgatattgc aactagagct gcaataaaaa gtgcaatgct
      361 gtttcttaat tgatcactca atcctgtaaa tgctactaaa aaccaaatta atagtattaa
      421 aagagacata cttcctgctg gttcaaagta aacaagaaga aagattggaa gtactgccaa
      481 gaaagatata aggaacaaaa tccattgatt gtactttccc ttaaatgaga aaatacatgc
      541 tgttgtaagt ataacagtga ccttggctat ctcagagggc tgtaactgaa ctccaccaat
      601 aacaagccat ctttgcacat tattaattac aggaccaaat aagtatacac agataagtaa
      661 aatcaaagtt aaaatatata ttgggataat aatctgagta tatttaaaca agcttgcatc
      721 aaacagagaa attaaaaaat atataataaa accaattaaa agaaatataa cttgcttttg
      781 tacaatacta acatctccaa actgtccatt actatcaata gttgttgata acaaagtaac
      841 tatacctata aatgagataa gtatagatgg aattaacaaa tatggatctg ctttaatcct
      901 ttttatcttt ttactttcta acttcattaa tactaatgat actaccagaa aagtagaagg
      961 agagaaacac ttttacttgt attattcctt ctttttaaat atttttctca taaaagcagg
     1021 tttatcaaga gggtcttcaa aatcatctgg ttgtggaata gtatcaataa agctctcttc
     1081 ctctttttct acattcactt cagaagtttt ttcttcttca aatgacttaa tctcatcagt
     1141 aactccttct aagccaaata tatctgtttc atcttcctca cctttttgtt tctttgtata
     1201 tacctgcttc ttttcatcaa aacctgtagc aacaattgta atgacaattt catcacctaa
     1261 tgaaggatct atctgtgctc caaacttgat atttgcagaa ggatctactg cctctctaat
     1321 caactcagat acaagactaa tctctggtaa agtaagatct gctcctacaa tattgtaaag
     1381 aactcctgtt gctccctcta tagacatatc aagcaaagga ctagttgtag ccgcccttgc
     1441 cgcaagttct gccctctttt ctccacttgc cctacctata cccataagtg ctgtaccagc
     1501 attttgcatt actgattttg catcattaaa gtcagcattt atatatccag tctggctgat
     1561 aagcattgaa atactattaa ccccttctgc aagtacagag tctaccatct tcattgcatc
     1621 tataaaggaa atattatcag tagcaatctc taacaacctt tgattaggaa caattattag
     1681 cgtatcaact ttatccctta aattattcaa accttcctct gcaacctcca ttcttctttt
     1741 accttcaaag tcaaaaggct ttgtaacaac agcaactgta agtgccccca tattttttgc
     1801 aacaccagca ataataggag cagcacctgt acctgttcct cctcccattc ccgctgtaat
     1861 aaacaccata tcagctcctt caagtaactc ctgtatctgg tcaagacttt cttctgctgc
     1921 ttgtgcacct agctcatgat ttcctccaac acctaaacct ttggttaaat tttcccctaa
     1981 ctgcagcgtt actggagctt tagaaagctt aagagtttga gcatctgtat taaaagccat
     2041 aaactcaaca ccagggatat cataatcagt catcatggtg ttaatagcat tacaccctgc
     2101 tcccccaact ccaattactt ttatttttgc aactggatca acatttgttg taattaaagg
     2161 cataacttaa acacaattaa taatttaatc taggcagttt atggcattaa agacttaaac
     2221 cattctacta tcttatcctt taaatttcca ccacctttta ccttcatagt ttctgattct
     2281 ccgtcctctt ctaatgcata ttttatcaat ccttggacac atgcgaaaga tgggtcagaa
     2341 atttcctcta tcatacctgt taaaccagat ggatacccta ctcttgcagc aacaccaaac
     2401 actccctgag taaacttagg taagtcccta agaagcgaag ttccacctgt taaaacaatt
     2461 ccagcaggca ttgccatttt aaaaccagct tttccaactt tctcctttgc catatcaaat
     2521 atctcctcac accttgcctc aacaatcttt acaagcagct ctttagaaac cttcttacct
     2581 gtaatatcta gaacagacaa atctatctca tcaccctttt ttgtctttgg ctcttcctca
     2641 tctaattttc taagaagcgc aggtgttgag tcagaagaag atttcttagc ttttgcttta
     2701 ttatctaaca aatctcccat acgaatcttt attttctcgg cttcttcaag tgatacctga
     2761 agcccaattg ctaaatcact tgtaatattt attcctccga gaggaataac accgctatat
     2821 attattgacc cctcatcaaa tattgatata ctcgttgttc cagcaccaat atcaagcata
     2881 gtaacaccaa gctccttttc agtatcagtt agaacagaca aagaagatgc ccatcctgta
     2941 aatacaacat catatacatt aagtccaag
//
LOCUS       DAOSAB010000063         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_22198_length_2963_cov_2.788171, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000063 DAOSAB010000000
VERSION     DAOSAB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22198_length_2963_cov_2.788171"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2963
                     /locus_tag="PK432_01695"
     CDS             <1..>2963
                     /locus_tag="PK432_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59670.1"
                     /translation="AITEHYDEHLSDIVDENYLTEETKKVLASNKITEIEIMIENLTG
                     NKQSLGEVSSEIQKLKNTISELPEDSTDKRDLNNKLKLLERKQRSITRVLKINSIISV
                     LHDKGMLKNPNYNIVSYVHQNNSLKALGNILSNITVFNKLYINQRSSTQAMKDALLKF
                     GKQMKLENFVPDGNMYSPAYVVASKLINSSGKKGIEQTASINKFFALANQFNLKLKPE
                     QVIWAFTTAKDDKSAVKKIYDNYGTMVDSYVDGEIIENNKRVIEIIGNILGMFADAAK
                     DPIPNALGFNEVNTSVALSMIGLGLPLEFVLGFNFIGSIRNAVANMQAASYGITEDIN
                     TKTVYFSDMLEEQIAKLKLKDPNIIIYDIKNIDSDKLQIDWKAPEGDGISPQDVLKNT
                     LLPSDVGYTVSYDGKVLSPEAQEFILLSMYKAQVQQIGKILPINAATSGLMKSLNPDT
                     LTLDKIITTIKDLKAKKEDSIFTDDSIDRLFADDKVFNVLTKVVADLSNQLDKMFIER
                     SPMFKSLVRSFSRFYDTGQEIANRFVGFLALRAYLNTYMVKEANDPNINDYYKQYIRK
                     DQDNLKKAFSIYGMYDTAFIEELNQMKAKYPDNEFLSLLHVDKTGNPIYIDGKIVHQQ
                     NIRIFATTKLKNSEYLSKVINDMQALFKHANLDVRLFMNHLFFKELARTGMQPNVTGQ
                     YLHFFPEMLSKVSQPIDTLMELLINPDAKADTIINTMKELYNINSEEELEVLFTDLVA
                     QMINGINPENVNFKDRSNINLAKIRTAFPNYTIDQIVDMARTIFGNSIQIPDKAVSIP
                     LSNYNKDNIELNFDFSTDYMEKNSGAISLLASRMNVFYDNVEKTFIFPPIMKINKQVF
                     VISKIDGTTTTIGKNLVESFIQGKEFDLKGSKAEYVVFDIPVNKSVISNPVHFTLQDM
                     IDLRNAATNKISTSTETITQPTTQFERKNIFTVTPIQAVDKKAKAIEQLFNENPELAN
                     SVYEALGFKVGSVNF"
BASE COUNT         1219 a          343 c          441 g          960 t
ORIGIN      
        1 tgcaataaca gaacattatg atgaacattt atcagatatt gtagatgaaa attatcttac
       61 agaagaaact aaaaaagtct tagcttctaa taagataaca gaaatagaaa ttatgattga
      121 aaatttaaca ggtaataaac aaagtcttgg tgaagtaagt tctgaaatcc aaaaattaaa
      181 aaatacaatt tctgaattac ctgaagatag tactgataaa agagatctta ataataaatt
      241 aaaattatta gaaagaaaac aaagaagtat tactagagta ttaaaaataa attctattat
      301 aagtgtacta catgataaag gaatgttgaa aaatcctaac tataatatag taagttatgt
      361 acatcagaac aatagtctaa aagctttagg aaatatatta tcaaatataa cagtttttaa
      421 taaactttat ataaatcaaa gatcatctac acaagcaatg aaagatgcat tattaaaatt
      481 tggtaaacaa atgaagttag aaaactttgt accagatggt aatatgtata gtcctgctta
      541 tgttgtggca tctaaactta taaactcaag tggtaaaaaa ggtattgaac aaactgcttc
      601 tataaataaa ttctttgctt tagcaaatca atttaatctt aaattaaaac cagaacaagt
      661 tatatgggct tttactacag ctaaagatga taaatctgct gtaaaaaaaa tatatgataa
      721 ctatggtaca atggtagata gttatgtaga tggtgaaata atagaaaata ataaaagagt
      781 tatagaaatt attggtaata tccttgggat gtttgcagat gctgctaaag atcctatccc
      841 aaatgcttta ggatttaatg aagtaaatac ttctgtagca ttatcaatga ttggactagg
      901 actaccttta gagtttgtat taggttttaa ttttattggt tcaataagaa atgctgtagc
      961 aaatatgcaa gcagccagct atggtatcac agaagatatt aatacaaaaa ctgtgtattt
     1021 tagtgacatg ctagaagagc aaatagcaaa attaaaatta aaagatccaa atattattat
     1081 atatgatata aaaaatattg attctgataa attacaaata gattggaaag ctcctgaggg
     1141 agatggcata tctccacaag atgttcttaa aaatacatta ttaccttctg atgttggtta
     1201 tactgtatct tatgatggga aagtattaag ccctgaagca caagagttta tattacttag
     1261 tatgtataaa gctcaagtac aacaaatagg taaaatactt cctattaatg ctgctacatc
     1321 tggattaatg aagtcgttaa atcctgatac attaacatta gataaaataa taactactat
     1381 taaagattta aaagccaaaa aagaagatag tatatttaca gatgattcaa ttgatagatt
     1441 atttgctgat gataaagtat ttaatgtact aacaaaagta gtagctgatt tatcaaatca
     1501 gttagataaa atgtttattg aaaggtctcc tatgtttaaa agtcttgtta gaagttttag
     1561 tagattttac gatacaggac aagaaatagc aaacagattt gttggatttt tagcattaag
     1621 agcttactta aatacttata tggtaaaaga agctaatgat cctaatataa atgattatta
     1681 caaacagtat attcgtaaag atcaagataa tcttaaaaaa gcattttcta tatatggaat
     1741 gtatgataca gcctttatag aagaattaaa ccaaatgaaa gctaaatatc ctgataatga
     1801 gtttttatct ttattgcatg ttgataaaac aggaaaccca atttatattg atggaaaaat
     1861 tgtacatcaa caaaacattc gtatctttgc aacaacaaaa ttaaaaaatt cagaatattt
     1921 gagtaaagta attaatgata tgcaagctct atttaagcat gcaaatcttg atgtaagatt
     1981 atttatgaat catttattct tcaaagagtt agcaagaaca ggtatgcaac caaatgttac
     2041 aggtcaatac ttacattttt ttcctgaaat gttgtcaaaa gtatcacaac caatagatac
     2101 tttaatggag cttttaatca atccagatgc taaagcagat actattatca atacaatgaa
     2161 agaattgtat aatataaact ctgaagaaga attagaggta ttatttacag atttggtagc
     2221 tcaaatgatt aatggtatta atcctgagaa tgtaaacttt aaagatagaa gtaatattaa
     2281 tttagctaaa ataagaacag cttttccaaa ttatactata gatcaaatag tagatatggc
     2341 tcgtactata tttggtaatt caattcagat tccagataaa gctgtttcta ttccattatc
     2401 aaactataat aaagataata tagaattaaa ctttgatttt tctactgatt atatggaaaa
     2461 gaatagtggt gcaatttcac tattagcatc aagaatgaat gtgttttatg ataatgtaga
     2521 aaaaacattt atatttcctc ctataatgaa aattaacaaa caagtgtttg taattagtaa
     2581 gatagatggt acaacaacta ctattggtaa aaacttagta gaatctttta tacaaggaaa
     2641 agaatttgat ttaaaaggta gtaaagcaga atatgttgta tttgatatcc cagttaataa
     2701 aagtgtcata tctaatcctg tacattttac attacaagat atgatagatt tacgtaatgc
     2761 tgctaccaat aaaattagta cttctacaga aactatcaca caacctacta ctcaatttga
     2821 aagaaagaat atattcacag taaccccaat tcaagctgta gataaaaagg ctaaagcaat
     2881 agaacaatta tttaatgaaa atcctgaatt agctaatagt gtatatgaag ctttagggtt
     2941 taaagtggga agtgttaatt tca
//
LOCUS       DAOSAB010000064         2919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_22645_length_2919_cov_2.823673, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000064 DAOSAB010000000
VERSION     DAOSAB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22645_length_2919_cov_2.823673"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2919)
                     /locus_tag="PK432_01700"
     CDS             complement(<1..>2919)
                     /locus_tag="PK432_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59671.1"
                     /translation="NYLKANLAEGVQENLQEAISRGAVEHAAALFKDPIRGAYEGYMP
                     HFMKGLKEQFTTEGAETFAGGFAMSLFAQPIMGGAGITVGKILNATVNKENYKALEEA
                     RKRERNGYIDERTGQEVKGNIQFLNELLQDTLLHLSPDLENAIRTGKLSSDMFTAARV
                     GKKKEALDSRQAIQNHHIITAIRTGKLDIILSELKEKTKLQGKELVEAFEKYGVKEED
                     VDKVIPLIDGIISRAKTLERDYNDAANKYPNPYNPSIYRNDVGQYLAAVSAKKAWDEA
                     VYNLVFAKATFESNSQRIADIAKTFSTISNELAESDAQGMMTLLSSGSVINEINMLRN
                     EIKTLSEDNKEQKKTKQDKQDKLERLGQFYDSLLNAKAAKTDDEKSKAEEESKTAFMN
                     YVKYLAGKNGNVVFNKDIENAYQMAKDHMLLRDDNAGLAESINVLMTPKSFLNFQKRL
                     FTTYKDLLSSREDILAMNNDDFEKRTGVITLLNKIKDTGLIIPQDTVADIWEAHQNNT
                     EIPLPEYFLDSNGNQITSGPQYEQAMRMLDAYKDIFKKAATPEETIKEKYDENDISTY
                     PKDLIDVLEGVYLEHHKFNPDSKYGLKKDDGTPLSKEEYAALSSQEQEAYEKQNKLNF
                     ERYVLESPQAKVWKKQYFNQLKESQTEVPEKYRNLTLDQLKEEKAKLLEQVKINTALE
                     ADLMIVEDQIAVKTMRAKTPEQDQAIRTVKSLSKDIELTEDETGYTKDGVKSDYRVTS
                     LAQEILEKEYGAPSFKDFTDRDTVAAVMELAKQFIEGGKSSNQSVESALKAWYEKAKG
                     LSIGYKQKLSNRIDPGKLKRIINAMKTLDYDKFKELLDQEAYSESTTAGTIIDDLVRD
                     FFDGRKMVKPEEMSPEAFSSLRKALHNLMEQVHKNEDVIIAKNLKLFGEGVVNGKKVK
                     IAGAMDLVAITPDGTIKIYDLKTSKSWEYYGTNEDLFKTKEKYALQLSLYKNLLENAT
                     "
BASE COUNT          843 a          549 c          389 g         1138 t
ORIGIN      
        1 ctgtagcatt ttctaataag tttttatata aagataattg taaagcatac ttttctttag
       61 ttttaaaaag atcctcatta gttccataat actcccagct tttactggtc tttaagtcgt
      121 atatctttat tgtaccatca ggagtaatag caaccaagtc catagcaccg gctatcttga
      181 ctttcttacc atttactaca ccttctccaa ataacttaag attttttgct ataattacat
      241 cttcattctt atgaacttgc tccataagat tatgtaaagc ttttcttaaa ctactaaaag
      301 cctcaggaga catttcttca ggcttaacca ttttcctacc atcaaagaaa tctcttacta
      361 gatcatctat gattgttcct gcagtggtag attcactata agcttcttga tctagaagtt
      421 ctttaaactt atcatagtct aaagttttca tagcattgat tattctctta agctttcctg
      481 gatcaattct atttgaaagc ttttgcttat atcctataga taaacctttt gctttttcat
      541 accaagcttt taatgcagac tctacacttt gattagaact tttaccacct tcaataaact
      601 gtttagccag ttccattaca gcagctacag tatctctatc cgtaaagtcc ttaaaagaag
      661 gagcaccata ttctttctca agaatttctt gagctaaaga agttactcta taatcagatt
      721 taacaccatc tttagtgtaa cctgtttcat cttctgttaa ctcaatatcc tttgataaag
      781 attttacagt tctaatagct tgatcttgtt caggagtttt agctcgcata gttttaactg
      841 ctatctgatc ttcaacaatc attaagtcag cctctaaagc tgtattaatt ttaacctgtt
      901 caagtaattt agctttttct tctttaagct gatctagtgt aagatttctg tatttttctg
      961 gtacttcagt ttgcgattct tttaattgat taaagtactg ctttttccat actttagctt
     1021 gaggagattc taaaacatat ctttcaaagt ttaacttatt ttgtttttca taagcttctt
     1081 gctcttgaga agataaagct gcatactcct ctttagataa aggtgtacca tcatctttct
     1141 ttaatccata tttactatca ggattaaact tgtgatgttc taaatagaca ccttcaagaa
     1201 catctattag atctttagga taagttgaga tatcattctc atcatatttt tcttttatag
     1261 tttcttctgg agtagctgct tttttaaaga tatctttata agcatcaagc attctcatag
     1321 cttgctcata ctgaggacct gaggttatct gattaccatt agagtctaag aaatattctg
     1381 gtaaaggtat ctctgtattg ttttgatgag cctcccaaat atcagcaaca gtatcttgag
     1441 gtattataag tcctgtatct tttattttat ttaaaagagt tataacaccg gttctttttt
     1501 caaaatcatc attattcatt gctaagatat cttctcttga agaaagaaga tctttatatg
     1561 tagtaaataa tcttttttga aaatttaaga aactcttagg agtcattaag acattgatag
     1621 attcagccaa accagcatta tcatctctaa gaagcatgtg gtcttttgcc atctggtaag
     1681 cattctcaat atctttatta aatactacat tgccattttt accagcaagg tatttaacat
     1741 agttcataaa agctgtctta gattcttctt cagcttttga tttttcatca tctgttttag
     1801 cagctttagc atttagtaaa ctgtcataaa actgaccaag tctttctaat ttatcttgtt
     1861 tatcttgttt tgtttttttc tgctccttat tatcctcact aagagttttt atctcatttc
     1921 tgagcatatt aatctcatta ataacagatc cagaacttag aagagtcatc ataccttgag
     1981 catctgattc agctagttca ttagatatag tagagaaagt ttttgctata tcagcaattc
     2041 tttgactatt tgattcaaaa gtagccttag caaatactaa attatataca gcttcatccc
     2101 aggccttttt agcagaaact gctgctaagt actgacctac gtcatttcta tatatagaag
     2161 gattgtatgg attaggatat ttattagcgg catcattgta atctctttca agagttttag
     2221 ctctgctgat aataccatct ataagaggta ttaccttatc tacatcttct tcttttacac
     2281 catacttctc aaaggcctct acaagttctt taccttgcag ctttgttttt tcttttaatt
     2341 cactcaaaat aatatctaac ttgccagttc taatagctgt tattatatgg tgattttgta
     2401 tagcttgtct actatctaaa gcttctttct ttttacctac tctggctgca gtaaacatat
     2461 cactagaaag tttaccagtt ctgatagcat tctctagatc aggggataaa tgcaataagg
     2521 tatcttgtaa taactcattt agaaattgta tgttaccttt tacttcttga ccagttcttt
     2581 catcaatata tccattacgt tctctctttc tagcttcttc aagagcttta tagttttctt
     2641 tatttacagt tgcattaagt attttaccta ctgtaatacc tgctcctccc ataataggtt
     2701 gagcaaacaa actcatagca aaacctccgg caaaagtttc agccccctct gtagtgaatt
     2761 gttcttttaa tcctttcatg aaatgaggca tgtaaccttc atatgctccg cgaataggat
     2821 ctttaaataa agcagctgca tgttcaacag cacctctaga tatagcttct tgtaagtttt
     2881 cttgaacccc ttctgctaga ttagctttaa gatagttca
//
LOCUS       DAOSAB010000065         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_22829_length_2902_cov_2.912891, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000065 DAOSAB010000000
VERSION     DAOSAB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22829_length_2902_cov_2.912891"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PK432_01705"
     CDS             <1..1089
                     /locus_tag="PK432_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOK59672.1"
                     /translation="ASPSTLKLPENTVLIIESTSTKKDTLDEIFNRLGGFIRYGEIID
                     NLNTFLNPYFEKEKITFGISILGESELKKQDIEKLANEIKRGFKGSNISSRFILPKEF
                     ELNAAQIFNNDVLNSGFELCIFENEKKRVYGKTLKVQDINSFIKRDIQRPEVDFEMGV
                     LPQKLARIMCNLTGLQSGIIWDPFCGSGTILMEAAVLGFDVLGSDIDVNALNLANENM
                     IWLSKEGIIKGIKYNLFQLDIYNVEKKVITDLKHTGINAVVCEPFMGPPQRKILSVSK
                     ADLLIENATKLYFSLFHVLNEISQKGFRVVIVLPSYKTNKGWIDMNISQFINKKWEVL
                     NKKYSSQDLKWKRINSIIARNIFILEKK"
     gene            1112..1378
                     /locus_tag="PK432_01710"
     CDS             1112..1378
                     /locus_tag="PK432_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOK59673.1"
                     /translation="MSHVVGAGSVAMGGNVAGKRLGVKKFEGEKVKAGNIIVKQRGSV
                     YHPGKNTYLSKDYSIHAKTDGFVKFRKMSGFKRGRYYIDVVESI"
     gene            1384..2127
                     /gene="rlmB"
                     /locus_tag="PK432_01715"
     CDS             1384..2127
                     /gene="rlmB"
                     /locus_tag="PK432_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOK59674.1"
                     /translation="MSKERFIQIESKNALVELLREGRDFERIYIASSAYRDDKTKEIV
                     KLAGEKKIPILRVPRKALQRRLRSSSSESVVGMMFSQNQWNLDELLESIYSEKKIPFF
                     LILDDIKYTQNIAAIMRTAFATGVNGIITQPSNVNLVTDETIRISMGAAERIPLVEMN
                     LFMAIKKLKKEGIKIYGIHMEGETYFKADLTGPIAFVLGAEDSGISSGMLERVDKTLS
                     IPMREGIGSLNVSASAAVIMYEKLRQEVS"
     gene            complement(2128..>2902)
                     /locus_tag="PK432_01720"
     CDS             complement(2128..>2902)
                     /locus_tag="PK432_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOK59675.1"
                     /translation="KILPNPYQPRMKIDPDDLIGLADSIRVNGVIQPLIVTKDSNSDK
                     YFIIAGERRYRAAQLAGLKYVPVVIKESSPQQMLELALIENIQRKDLNPLEEAHAFAQ
                     LQDEFGMKQSEIGKKLGISRVSVTNKRRLLNLPESVQEMVLNETITEGHARALLGIKD
                     PESIVAAADIVVNRKLSVRQTEELVRKVIFGKNPRYRTFQDETPDLVRYARMITETIG
                     YNTRIKKLAKGGRVIIKYESKQQLEELLKKLGIDKPNEF"
BASE COUNT          969 a          394 c          573 g          966 t
ORIGIN      
        1 gcctcaccca gcactttaaa attacctgaa aacactgttt taataattga aagtacgagt
       61 actaagaagg atactctcga tgagatattt aatagactag gcggatttat tagatatgga
      121 gagataatag acaatcttaa tacattctta aatccatatt ttgagaaaga gaaaataaca
      181 tttggtattt ctatacttgg agagagtgag ctaaaaaagc aggatattga gaaacttgct
      241 aatgagatta aaagaggttt taaagggagt aatatatctt cccgctttat tcttcctaaa
      301 gaatttgaac ttaatgcagc acagatattt aataatgatg tgttaaatag tggatttgag
      361 ctgtgtattt ttgaaaatga aaagaaaagg gtatatggta agacattaaa agtacaagat
      421 attaactctt ttattaaaag agatatacaa agaccagagg tcgattttga aatgggagta
      481 cttcctcaga aacttgcaag aataatgtgt aatcttacag gtttgcaaag tggtattatt
      541 tgggatcctt tttgtggtag tggtactatt cttatggaag ctgctgtact gggttttgat
      601 gttttaggct ctgatattga tgttaatgca ctaaacctcg caaatgagaa tatgatatgg
      661 ctctcaaaag aagggattat aaaagggata aagtataatc tgtttcagct ggatatttac
      721 aatgttgaga aaaaagttat tactgatttg aagcatacag gaataaatgc tgtagtatgt
      781 gagccgttta tgggtcctcc tcaaaggaag atattgagtg ttagtaaggc ggatttgtta
      841 atagagaatg caacaaagct ttatttctct ctttttcatg ttttaaatga gatatctcaa
      901 aagggtttta gagttgtaat tgttcttccc tcttacaaaa caaacaaagg atggattgat
      961 atgaatattt ctcagtttat taataaaaaa tgggaggttt taaataaaaa atactcttca
     1021 caggacttga aatggaagag gataaatagt attattgcaa ggaatatttt cattcttgaa
     1081 aagaagtaat taaattgaat tatatatagc aatgtcacat gtagtaggag caggaagtgt
     1141 tgcaatgggg ggaaatgttg ctggtaagag actaggtgtt aagaagtttg aaggagaaaa
     1201 agttaaggca ggaaatataa tagtaaagca aaggggatct gtttatcatc ctggtaaaaa
     1261 cacatatctt tccaaagact actctattca tgctaaaact gatggttttg ttaaattccg
     1321 aaaaatgagt gggtttaaaa gaggccgtta ctatatagat gtggtagaat ctatatagaa
     1381 aatatgagta aagaaagatt tatccaaata gagagtaaga atgcattagt tgaactgcta
     1441 agagagggca gggattttga gcgtatatat attgcaagca gtgcatatcg cgatgataaa
     1501 acaaaagaaa tagtcaaact tgctggtgag aaaaaaattc caatacttag agttccaaga
     1561 aaagcacttc agagaagact tagaagttca agttcagaga gtgtagtagg gatgatgttt
     1621 tcacaaaatc agtggaattt agatgagtta ctagaaagta tatacagtga aaagaaaata
     1681 cccttctttt tgattcttga tgatattaaa tatactcaaa atattgctgc aataatgaga
     1741 accgcttttg ctactggggt aaatgggata attactcaac cttctaatgt aaatttagtg
     1801 actgatgaga ctattagaat atcaatgggt gctgctgaaa gaataccgct tgtagagatg
     1861 aacctgttta tggcaataaa aaaacttaaa aaagagggaa taaaaatata cggtattcat
     1921 atggagggtg agacatactt taaggcagat ttaactggtc ctattgcttt tgtcctagga
     1981 gcggaggata gtggtatatc gtcaggaatg cttgagaggg ttgataaaac actttcaata
     2041 cctatgagag aaggtatagg gtcattaaat gtgagtgcta gtgcagctgt tataatgtac
     2101 gagaagctta gacaagaggt ttcttagtta aaattcatta ggtttatcaa tacccaattt
     2161 tttcaataat tcttcaagtt gttgttttga ttcatacttt attattactc ttcctccttt
     2221 agctaatttc tttatacgag tattgtatcc aattgtctca gtaatcattc ttgcatatct
     2281 gactaaatca ggagtttcat cctgaaaagt tctgtatcta ggatttttcc caaatattac
     2341 ttttctgact agctcttctg tttgtcttac acttagcttt ctatttacaa caatatctgc
     2401 tgctgcaaca atagactcag ggtctttaat cccaagaagt gctctagcat gtccttctgt
     2461 tattgtttca tttaatacca tctcttgtac tgattctggg agatttaaaa gtcttctttt
     2521 gtttgttact gaaactctgc taatacctag ttttttccca atttctgact gtttcattcc
     2581 aaattcatct tgaagctggg caaaggcatg agcctcttcc agtggattta aatcctttct
     2641 ttgaatgttc tctattaaag caagttcaag catctgttgc ggagatgatt ctttaatgac
     2701 cacaggaaca tatttaagac ctgcaagctg tgctgctctg tatcttcttt cacctgctat
     2761 tatgaagtat ttatctgaat tagaatcttt tgttacaatt agtggctgta taactccatt
     2821 tacccttatt gaatcagcta aaccaatgag atcatctgga tcaatcttca ttcttggctg
     2881 atatgggttt ggcagtattt tg
//
LOCUS       DAOSAB010000066         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_22905_length_2894_cov_3.767524, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000066 DAOSAB010000000
VERSION     DAOSAB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22905_length_2894_cov_3.767524"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PK432_01725"
     CDS             <1..877
                     /locus_tag="PK432_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59676.1"
                     /translation="IGIAPVSQTLKLSPGEIYSDEIVYWNTSQTVNTYKVFVSGFTQI
                     ENQPGTAVILTPEDDAKAPYSASKWVTVDKDTLTLEPNKNVKLKYTITVPKDVTNGEY
                     NVEIFLISESDFAQAGTAAFTNLASGTPILIQIGDEFVENAELLKFITDKDIYEKINV
                     KFLTRLKNIGDTHIKPTGEIVIENIFGKKVATIPFNKTSQSLMRDTIGDYEDNWSQSS
                     YISPNKAIAVGPMTAKLIVTYRSYQPGFAVLEAETTFWIIPWKIIIAILLVIILIIAY
                     NIYRKNNKKKKNEIH"
     gene            864..1754
                     /locus_tag="PK432_01730"
     CDS             864..1754
                     /locus_tag="PK432_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59677.1"
                     /translation="MKFIRTLLMTIMIYTIIGISFIYADSITPGVIRTTLSAGERKYE
                     SVTFTNEQNNTVELSIKPYTYNPQTDEITEDTKDIFIKADTDTIKVHGKSSVDIKYEI
                     YPLDNIEEGTYFNILVIAPVISNKKIDISPSISQLVILDIVSPQNQVKGIMTDRYTID
                     VEVAKKGIPFISPMVIKYKITNNSNYLLIPQGRIDIFNEKNTYKSIYVYINPENQKLY
                     PAQTLENEVKISQWHISDLFLNRVVKGEIYNGIDGVPKPIETEVKSFTLGIIFLFVAI
                     LLSIFLVKSIQKDKKRSKKD"
     gene            complement(1751..2647)
                     /locus_tag="PK432_01735"
     CDS             complement(1751..2647)
                     /locus_tag="PK432_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOK59678.1"
                     /translation="MSNQNTVTVQFQKTDYQRVKDTLTAYGWREESDENQYVVYRLRS
                     PKGSIAIMYFSGKLVFQGREDFTSLISSLQNGSSFNEEFVPHIGVDEVGKGDYFGPLV
                     VVSCFVNEDFFKKVKLLGFADSKKFNDEKIERMFNMVRDYPYYYSSIVYPKDYNRMAK
                     EYKNASVLLAKQHSKVIEIGLNDLEKKGIECSYVVIDQFSPIKSRVINELGPLGKHIK
                     FIQFHKGESDIAVAAASIIARGIFIEEWRKMEEKYNFHFPKGATLVVDNAKLFVSRYG
                     EKELNNVAKVGFKTTNEVLNKV"
     gene            complement(2649..>2894)
                     /locus_tag="PK432_01740"
     CDS             complement(2649..>2894)
                     /locus_tag="PK432_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006284840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HOK59679.1"
                     /translation="LEKRIKKELEDNDRRIELIVAVNPSLEGEATASYIQDLFRKYVK
                     EDRMHISRLAMGLPTGADLEYADRLTLKKALEGRRDI"
BASE COUNT         1100 a          523 c          383 g          888 t
ORIGIN      
        1 aataggtatt gcaccagtat cacaaaccct caaactatct cctggagaaa tatattctga
       61 tgaaattgta tattggaata catctcaaac tgttaataca tataaggttt ttgttagtgg
      121 ctttacacaa attgaaaatc agcctggtac tgcagtaata cttacaccag aagatgatgc
      181 taaagcaccc tactctgcat ctaagtgggt aactgttgat aaagacacat taacacttga
      241 accaaacaaa aatgtaaagt taaaatacac tattacagta ccaaaagatg taaccaatgg
      301 agaatataat gttgaaatat ttttaatatc tgaaagtgat tttgcacaag caggaacagc
      361 agcatttaca aatctcgcat caggaacacc aattttaata caaataggag atgaatttgt
      421 tgagaatgca gaactcctaa agttcattac agataaagat atatatgaga agataaatgt
      481 aaaattcctt acaagactta aaaacatagg ggatacacat ataaagccaa ctggggaaat
      541 tgtaatagaa aatatctttg gtaagaaagt agcaacaata ccatttaata aaacaagcca
      601 aagtctcatg agagatacaa ttggagatta tgaagacaac tggagtcagt caagctatat
      661 atcgcctaac aaagcaatag ctgtaggacc aatgacagca aagctcattg taacatacag
      721 aagctatcag cctggatttg ctgtactaga agcagaaaca acattttgga taattccatg
      781 gaagataatt attgcaatac tccttgtaat tattctcata attgcctata acatttatag
      841 gaaaaataat aaaaagaaaa agaatgaaat tcattagaac tcttttaatg acaataatga
      901 tatataccat tattggtata tcatttatat atgcagatag tattactcct ggtgttataa
      961 ggacaaccct ttctgcagga gagcgaaaat atgagtcagt tacctttaca aatgaacaaa
     1021 ataatacagt tgaactttct ataaaaccat atacttataa tccacaaact gatgaaatta
     1081 cagaagatac aaaagatatc ttcattaaag cagatacaga tacaataaaa gtacatggaa
     1141 aaagctcagt tgatatcaaa tatgaaatat atccacttga taatatagaa gaaggtacat
     1201 actttaacat acttgtaata gccccagtaa tttcaaataa aaagattgat attagtccct
     1261 ctatctcaca gttagtaatt ttagatatag tctcaccaca aaatcaggta aagggtatta
     1321 tgacagatag atatacaata gatgttgaag ttgccaagaa aggaatcccc ttcataagcc
     1381 caatggtaat aaaatacaaa attacaaata actctaacta tttattaatt ccacaaggaa
     1441 gaatagatat attcaatgag aaaaatacat ataaatcaat atatgtatat ataaaccctg
     1501 agaaccaaaa actatatcct gcacaaacac ttgaaaatga agttaaaatc tctcaatggc
     1561 atatctctga tctattttta aacagagtcg ttaaaggaga aatatataac ggaatagatg
     1621 gagtgccaaa acctattgaa acagaagtaa aaagttttac attaggtatt attttcctct
     1681 ttgttgcaat actgttaagt atattcttag taaaatcaat acaaaaagat aaaaaaagaa
     1741 gcaaaaaaga ttaaactttg tttaagactt catttgtagt tttaaacccc actttcgcga
     1801 cattatttaa ctctttttct ccatacctag aaacgaaaag cttggcatta tcaactacta
     1861 aagtagcacc cttaggaaag tggaaattgt acttttcttc catttttctc cactcttcaa
     1921 taaaaatacc tcttgcaata atagatgcag cagcaacagc aatatcagat tcccctttgt
     1981 gaaattgaat aaatttaata tgcttaccaa gaggacctaa ttcgtttatt accctactct
     2041 taataggtga gaactgatct ataacaacat aactacactc aatacccttc ttctctaaat
     2101 catttagacc aatctcaata accttagagt gctgttttgc aaggagaaca gatgcatttt
     2161 tatactcctt tgccattctg ttataatcct ttggataaac aattgacgaa taataatatg
     2221 gataatccct taccatatta aacattcttt ctattttctc atcattaaac ttttttgaat
     2281 cagcaaaacc taacaattta actttcttaa agaaatcctc atttacaaaa caactaacaa
     2341 caacaagagg accgaaataa tcacccttac ctacttcatc tacacctata tgaggaacaa
     2401 attcttcatt aaaagaacta ccattctgaa gtgatgatat taaagaggta aaatcctccc
     2461 tgccttgaaa tacaagcttg ccactaaaat acataattgc aatagaccct ttaggacttc
     2521 ttaatctgta tactacatac tgattctcat cactctcctc tctccatcca tatgcagtta
     2581 aggtatcctt aactctttga taatctgttt tttgaaattg aaccgttaca gtattttgat
     2641 tggacatatt aaatatccct ccttccttcc aatgcctttt ttaaagtcag cctgtctgca
     2701 tactccaaat ctgctcccgt aggaagtccc attgccaacc ttgatatatg cattctatcc
     2761 tcttttacat actttctaaa aagatcttga atatagcttg ctgttgcttc accctcaaga
     2821 cttggattca ctgcaacaat taattcaatt cttctatcat tatcttctaa ctctttttta
     2881 attctctttt caag
//
LOCUS       DAOSAB010000067         2890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_22953_length_2890_cov_2.827513, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000067 DAOSAB010000000
VERSION     DAOSAB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22953_length_2890_cov_2.827513"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PK432_01745"
     CDS             <1..473
                     /locus_tag="PK432_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59680.1"
                     /translation="SSDNLGREKFVKSFWNSIVMFEPAEATSPYDLKVVTANNPEKET
                     YVEVKVRKYNLSFFCDPSTPPMMDLGKFLKLKRIYNETKAPIYYAISTSDGYWVMINL
                     SYRIKNNALYKVITKTAKVSTVEDKGYADKQIVEFQYNEKFDRIYNERGEKVCC"
     gene            485..850
                     /locus_tag="PK432_01750"
     CDS             485..850
                     /locus_tag="PK432_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59681.1"
                     /translation="MSVIRDYLTLEDGGKKRRIANDAYSYIRLMVEYILNKHYNQAYR
                     KCEDMLVVIVEKIMLSKIEIKKIESTKNLYSYLYKLIRNTAINYIRDNNKLNVTYVGT
                     YWDLSKVVYGYNELEDENK"
     gene            890..1237
                     /locus_tag="PK432_01755"
     CDS             890..1237
                     /locus_tag="PK432_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59682.1"
                     /translation="MAVLMVIMLIIIMVSRYDLYTKQLEIKEQINLVESALRVLENDI
                     SRQQMEIKTQLNKIEVELHLIRINSDDLHKDHKKIINSVFNSEYYNKKLLALIDDLGK
                     ELKKQIDNYTNIN"
     gene            1453..1734
                     /locus_tag="PK432_01760"
     CDS             1453..1734
                     /locus_tag="PK432_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59683.1"
                     /translation="MNTINTSKLKKTLKALKADASVSEITILNAEMHNELIEQINSGK
                     KVNMYIVLQLRIQIAKNLMELKKFNKKLSDESEDIDNFINGLKGKKEVR"
     gene            1737..>2890
                     /locus_tag="PK432_01765"
     CDS             1737..>2890
                     /locus_tag="PK432_01765"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HOK59684.1"
                     /translation="MGYLDKINSYVNDVLSNKIPSCQWVKKACERFHSDLNKGDYYFD
                     ENAVDDVISFIRKLKLNTTPKKPFLLEDWQLFIIANIYGLKSKLNNRRKYKYVYIEIP
                     RKNGKSELITALALYHFIFDELATITVSANSREQAKNVDFKKIKSFAHQLDRREKYII
                     PYFNSLKMNGRQNEIIVTSSDAKRLDGLNISVGIIDELHSAKDSGVYDVIKSAMGSRE
                     EPLLIVITTAGFDVDSFCYNLRNHITDILNGVTIDESQFGIIYTLDEGDDIADENNWV
                     KANPNLGVSLSIDFIRDEVNKAQNSPSETAGVLVKNFNLWQKKSSEKSWINEAYLEKV
                     FAPVNIEDYRGADCMVGLDLGTNTDLTCFSICIKVQNGEYVFFTKYYLPE"
BASE COUNT         1141 a          364 c          531 g          854 t
ORIGIN      
        1 taagtagtga taacttagga agagaaaagt ttgtaaagtc attttggaac agtattgtta
       61 tgtttgagcc agcagaagcc acttcacctt acgacctaaa ggtggttact gcaaataatc
      121 cagagaaaga gacttatgtc gaagtaaagg taagaaaata caacttatct ttcttttgcg
      181 acccatcaac accaccaatg atggatttag gtaagttctt gaaactcaaa agaatataca
      241 atgaaacaaa agcacctata tactatgcta tttccacctc tgatggttac tgggttatga
      301 taaacctttc atacagaatt aaaaacaacg ctctctataa ggtaattacc aagacagcaa
      361 aggtctcaac agtagaggat aagggctatg cagacaagca gatagttgaa tttcaatata
      421 atgaaaaatt tgatagaata tataatgaaa ggggagaaaa agtatgttgc taagtgatag
      481 caatatgagt gtaattaggg actatcttac tctagaagat ggtggtaaaa agagaagaat
      541 agccaatgat gcttattcct atataaggtt gatggtggaa tatatcctta ataaacatta
      601 caaccaagcc tataggaaat gtgaagatat gctggtggta atagttgaga agataatgct
      661 aagtaagatt gaaataaaaa agatagaaag cactaaaaat ctatactctt acttatacaa
      721 gctaattaga aacactgcaa taaactacat tagggataat aataaattga atgtaaccta
      781 tgtagggact tactgggatt taagtaaagt agtttatggt tacaatgagt tagaggatga
      841 gaataaataa aaactaaaac tttaataaaa ctttaaagat atgggaacaa tggcagtatt
      901 aatggtaatt atgttaatta tcataatggt tagtaggtat gatttatata ccaagcaatt
      961 agagattaaa gagcaaatta acttagttga gagtgctctc agagttttag aaaatgatat
     1021 tagtaggcaa caaatggaaa ttaaaaccca actgaataag attgaggttg aattacactt
     1081 aattagaatt aactctgatg atttacataa agaccataag aagataatta actcagtttt
     1141 taatagtgag tactacaata agaaattatt agcactcata gatgacttgg gtaaggagtt
     1201 aaagaaacaa attgataatt atacgaacat taattaaatt atttagtgtt tatataaatg
     1261 agtaccctca cttcgttctt atcttttagt aaaggagagt ttatggggga tagggatgta
     1321 aaaagttgaa aaccagcatt atatccaccc cccacggtcg accttaacaa aaatggtgtt
     1381 ttaaggttaa tttaagtaac acagtaccaa acagttacaa cattgataca aatatatcaa
     1441 aatcaataag ttatgaatac aataaacact agcaaactca aaaaaactct caaagcacta
     1501 aaggcagatg ccagtgtaag tgagataact attttaaatg cagaaatgca caatgagtta
     1561 atagagcaaa taaacagtgg taaaaaggtt aatatgtata tagtgctaca gttgagaatc
     1621 caaattgcta agaacttaat ggaacttaaa aagttcaata aaaaattaag tgatgaaagt
     1681 gaagatattg ataactttat taatggatta aagggtaaga aagaagtgag ataactatgg
     1741 gttatttaga taaaataaat agctatgtaa atgatgtttt aagcaataaa attccaagct
     1801 gccaatgggt taaaaaagct tgtgaaagat ttcattctga ccttaataaa ggtgattact
     1861 actttgatga gaatgcagtt gatgatgtta tttcctttat taggaaatta aaactgaata
     1921 caacacctaa aaaacctttt ctcttagaag attggcagtt gtttattatt gccaacatat
     1981 atggattaaa aagtaaatta aacaatagaa gaaaatataa atatgtttat attgaaatac
     2041 ccagaaagaa tgggaaatca gaactaatca cagcactagc attataccat tttatatttg
     2101 atgaattagc caccattaca gtatctgcca atagtaggga gcaagcaaaa aatgtggact
     2161 tcaaaaaaat aaagtcattt gctcatcagt tagatagaag agaaaagtat attataccct
     2221 actttaattc cttaaaaatg aatggcagac aaaatgaaat tatagtaact tcatctgatg
     2281 ccaaacggct ggatggttta aacatttcag taggcataat agatgaatta cattctgcca
     2341 aagatagtgg tgtttatgat gtaataaagt cagcaatggg ttctagggaa gaaccacttt
     2401 taattgtcat aacaacggct ggatttgatg ttgattcatt ctgttacaac ctaaggaatc
     2461 atattacaga tattcttaat ggtgttacca ttgatgaaag tcagtttgga attatttata
     2521 ccctagatga aggtgatgat atagctgatg aaaataactg ggtaaaggct aatccaaact
     2581 taggtgtatc actctctatt gactttatta gggatgaagt gaataaggca caaaactcac
     2641 catctgagac tgctggtgtg ttagttaaaa atttcaattt atggcaaaag aaatcatctg
     2701 aaaaaagttg gataaatgaa gcctatttag agaaggtatt tgcaccagtt aatatagaag
     2761 attatagggg tgctgattgt atggtagggt tagacttagg aacaaacact gacctaactt
     2821 gcttttctat ttgtattaag gttcagaatg gtgagtatgt attcttcact aaatactatt
     2881 tgccagaaga
//
LOCUS       DAOSAB010000068         2851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_23373_length_2851_cov_2.806867, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000068 DAOSAB010000000
VERSION     DAOSAB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23373_length_2851_cov_2.806867"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PK432_01770"
     CDS             complement(<1..489)
                     /locus_tag="PK432_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOK59685.1"
                     /translation="MKNNMLSKQPYKGTNDWYPQDMFLRNYLFNIWTRVAKKYGYEEY
                     DTAQLEDANLYRVKSGEELGGSQLYNFTDKGGREIALRPEMTPSLARIVALKKNELQF
                     PLRWFNIGRYFRYEKPQKGRKREFFQLNIDILGVQDITAEVEIILFALDVMKELNAPK
                     SSF"
     gene            589..>2851
                     /locus_tag="PK432_01775"
     CDS             589..>2851
                     /locus_tag="PK432_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOK59686.1"
                     /translation="MNASKAKKQGRRVTYTNRPISLSNYNRIKMYSSKNAGKKSNAKV
                     SGLSKKKAKSNKGKKFLYIFLGVCFFLLCAGLITVGIYLKNLQNSLPSPDELISRRSD
                     ESTKIYDRNNVLLYTVYGDQNREFVSIEDIPEHTKWAVLAAEDIEFYQHKGLDYMGIA
                     KALYQNLTKGEIVRGASTITQQLVKGTLLYDILGEEAYAQTYSRKIKEILITMQVEQT
                     FTKDEILQMYMNEIPLGGVNYGFQAASKAYFGKDVKDLTLAESALIAGLVQSPGIYSP
                     LFGTKPELAKVRQTYVLDQMLKHKELTGVTEEEIQKAKEEELVYRTAKIDIKAPHFVF
                     YIKQLLEDKYGIDRVERGGLKVTTSLDYSVQQIAEDEVTKGISKNGVPRGVYNGAAVV
                     MNPRTGEVLAMVGSVDFWNTTDPRVDGNVNVTTSERQYGSSAKPYTYLASFGKGFSTG
                     TLAPDIPMSFGKYAPKDWDGQFEGVGTLRRNFGRSRNVSAVYVLQTIGVEPLLQLTEK
                     LGITTLKNKANYGLSLALGSGEMKLIEHTAAFTVFANEGIKNPTVTILKVEDSKGNVL
                     EEAKFEEGTRVVDEKEIYLLNYILCDLGGFGDRLGISYSRVNGKNVCFKTGTTDGPRD
                     LATMMYHKNLVVGVWTGNNNNAIARNATSTAVPLPIAYGITKRLADVYKPELFTRPAG
                     IVSTAVCKDTGGIPAEGVTCDKEASIYIAGRAPQTDNRQVVEVCKSNGLIPTNLEAAR
                     QYGLVEQKVLLTFK"
BASE COUNT          947 a          418 c          606 g          880 t
ORIGIN      
        1 aaagctactt ttaggagcat taagctcttt cattacatct agagcaaata gtataatctc
       61 tacctctgca gtaatatctt gtactcctaa aatatcaata tttaactgaa agaactctct
      121 ttttcttccc ttttgcggct tttcatatct aaaatatctt ccgatattaa accatctaag
      181 aggaaattgt aattcattct tttttaatgc gactattctt gctaaggaag gtgtcatttc
      241 aggtcttagt gcaatttctc taccgccttt gtcagtaaag ttgtaaagct ggcttccacc
      301 taattcttca cctgatttaa ccctataaag attggcgtct tcaagctgag ctgtatcata
      361 ctcctcatat ccgtattttt ttgctactcg tgtccatatg ttaaagagat agtttcttaa
      421 aaacatatcc tgagggtacc agtcatttgt tcctttgtac ggttgttttg atagcatatt
      481 gtttttcatc tttaaattat atcatcttcc ctgtttattg aatattgaga ataaataata
      541 tatattagga aagagtataa taatcatcaa agaactatga aatatagtat gaatgcatct
      601 aaagcgaaga aacaaggacg aagggttaca tatacaaaca ggcccatttc tttaagtaac
      661 tataacagga taaagatgta tagtagtaag aatgctggaa agaaatcaaa tgcaaaagta
      721 tcagggctaa gtaaaaagaa agctaagtct aacaaaggaa agaagtttct ctatatattt
      781 cttggtgttt gtttttttct actctgtgca ggtcttatta cagtaggaat atacttaaaa
      841 aatcttcaaa actctcttcc ttctcctgat gaacttattt caaggaggtc tgatgagagc
      901 accaagatat atgatagaaa caatgtttta ctgtatacag tatatggcga tcaaaataga
      961 gaatttgttt caatagagga tattccagag catactaaat gggctgtgct ggcagctgaa
     1021 gatattgaat tttatcagca taaaggtctg gattatatgg gtatagcaaa agctttgtac
     1081 caaaatctta caaaaggaga gattgtaaga ggtgcgagta caattactca gcaattagta
     1141 aagggaacgc ttttgtatga tatccttggt gaagaggcat atgcacagac atattcaagg
     1201 aagattaaag agatattaat taccatgcaa gttgaacaga catttactaa ggatgaaatt
     1261 ttgcagatgt atatgaatga aattcctctt ggtggtgtta attatggttt tcaggcagct
     1321 tcgaaggcat attttggtaa ggatgtaaaa gatcttactc ttgctgagag tgcattaatt
     1381 gcaggccttg tacaaagtcc aggtatatat tctcctcttt ttggtactaa gcctgagcta
     1441 gcgaaggtta ggcagacata tgtacttgac cagatgctta aacataaaga gttaactggg
     1501 gtaacagaag aagagattca aaaagctaag gaggaggagc tggtgtacag aactgcgaag
     1561 atagatatta aagctcctca ctttgtcttt tacattaaac agttgctaga agataaatat
     1621 ggtattgata gggttgagag aggaggactt aaagttacta cttcgttaga ttattctgtt
     1681 cagcagatag ctgaagatga agttacaaaa ggtatatcaa agaatggcgt gccaagagga
     1741 gtgtataatg gtgctgcagt agttatgaat ccaaggacag gtgaagtttt ggcgatggta
     1801 ggatctgtag atttttggaa tacaacagac ccaagggtag atgggaatgt aaatgttaca
     1861 acaagtgaaa ggcagtatgg ttcttctgct aaaccgtaca catatcttgc atcctttgga
     1921 aaagggttta gtactggtac acttgcacct gatattccaa tgagttttgg taaatatgct
     1981 cctaaagatt gggatggaca gtttgaggga gttggtacac taagaagaaa ttttggaaga
     2041 tccagaaatg tttctgctgt atatgtcctc caaacaattg gtgttgagcc attgcttcaa
     2101 ttaacagaaa agcttggtat tacgacatta aaaaacaaag ctaattatgg attatctctt
     2161 gcacttggat caggtgaaat gaaattgatt gagcatactg ctgcatttac tgtatttgct
     2221 aatgagggta ttaaaaatcc tactgtaaca atacttaaag tagaagactc taaggggaat
     2281 gtactagaag aagcaaaatt tgaggagggt actcgtgtag ttgatgaaaa agaaatatat
     2341 cttcttaact atatattgtg tgatttagga ggatttggtg atagattggg tataagctat
     2401 tcgagggtta atggaaagaa tgtatgcttt aagacaggta caacggatgg gccaagagat
     2461 ttagctacta tgatgtatca taaaaacctt gttgtaggtg tatggactgg gaataataat
     2521 aatgcgattg caagaaatgc aacaagtact gcagtacctc ttccaattgc atatggtata
     2581 acaaagaggc ttgctgatgt ttataaacca gagctattta caaggccggc aggaatagta
     2641 tcaacagctg tatgcaagga tacaggaggc ataccagcag agggtgttac atgcgataaa
     2701 gaggctagta tatatattgc aggccgtgct cctcagacag ataacaggca ggtcgttgag
     2761 gtgtgtaaat caaatggttt aattcctact aatctagaag cagcaagaca gtatgggctt
     2821 gtagagcaga aagttttgct tacatttaag a
//
LOCUS       DAOSAB010000069         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_23728_length_2820_cov_4.564919, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000069 DAOSAB010000000
VERSION     DAOSAB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23728_length_2820_cov_4.564919"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..2599
                     /locus_tag="PK432_01780"
     CDS             149..2599
                     /locus_tag="PK432_01780"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HOK59687.1"
                     /translation="MDIVTAILIIIGISLIFGIIIVLLNGGKGDDVEVFQEVRDPVVM
                     QILVPRENDKTPLAAEQMFASIHGILGDNVRSVDLVSFEIESSGNGGIRFYAVVPRHL
                     AKFIEGQIYAQYPNADIAYVQDYTLSKGQNTQLFVTTGEIELEKDYIFPIKTFRNFDV
                     DPLAAITGAISDLKINQSAWIQILVRPVANYWQANSKKYISDIKEGKDPYAGGFWFNL
                     SKIFKGIGETLTTTGEASERKEVVKLQPGQEEELSEIELKMLKVGFEFKIRVVTKAST
                     QIESEQILRDIVASFKQFTTAHLNSFVHSRPKESGEQIYSKYVKRYLPSDTADIINIE
                     ELASLYHMPNISVETPNIAWSRSKKIEPPMNLPKAGDDNVTVFAETDYRGTRYEFGIK
                     KEDRRKHFYILGKTGVGKSTVFKNMFISDILHGDGACMVDPHGELVDELLDFIPPNRI
                     DDVIYLNPTDTQYPIGFNLLELKDKSQRDLIADGVVEVFHKQFGTSWGPRLQYILTNA
                     VATALEAQGTTLLSVTRLLTDRNYRKFILKQVNDPILIKFWEEEYAQMAQNPRLLAES
                     VSPIQNKVGRFISSAVTRNIIGQVKSTIDLRDIMDNKKILLVNLAQGKLGEETASLLG
                     GMIITRLQSTALERVDIPADQRNDFFLFVDEFQNFATDSFAKILSEARKFRLDLCMTN
                     QYIDQIPLTVRQAIFGNVGTLGSFVVSQADAAILEKEFAPSVTSEDLVSLDAHSMYIK
                     LCIDGMTSNAFSARSLPLRYEPFGLREQVIAQSREKYGTPKEVIEDKIARWSSQTYSD
                     SGNRSLRRKDENSNNQKE"
     gene            2664..>2820
                     /locus_tag="PK432_01785"
     CDS             2664..>2820
                     /locus_tag="PK432_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK59688.1"
                     /translation="MSGHSKWSTIKHKKELNDAKKGKVFSKLSVEITHAARKGGGDPE
                     MNPTLRLY"
BASE COUNT          936 a          378 c          588 g          918 t
ORIGIN      
        1 gtttcctgga acatatgaaa ttattgaagg gagaccatct tttatgacaa ctggggatat
       61 tgtaataata ctgggaaaag atattccaac tgtttattct ttttaatatc ttctaaatat
      121 ttgttttcta atgtaaaatg aaatagttat ggatatagtt acagcaattt taataattat
      181 tggcatatca ttgatatttg gtatcatcat tgttcttctt aatggaggaa aaggtgatga
      241 tgtagaggtt tttcaagagg ttagagatcc tgttgttatg cagatactgg tacctaggga
      301 aaatgataaa actcctcttg cagctgagca gatgtttgct tctattcatg gtatattggg
      361 ggataatgta aggtctgttg atttagtgag ctttgaaata gaatcaagtg gaaatggtgg
      421 aattagattt tatgcagtag tacctaggca tcttgctaag tttattgagg gtcagatata
      481 tgcacaatat cctaatgcag atatagcata tgtgcaggat tatactttaa gcaaaggcca
      541 gaatactcaa ttgtttgtta caacaggtga gatagaacta gaaaaggatt atatttttcc
      601 tattaaaaca tttagaaact ttgatgttga tccattagct gcaattacag gggcaatatc
      661 tgatttaaaa attaatcaga gtgcatggat tcaaattctt gttagacctg ttgctaacta
      721 ttggcaggca aattcaaaga agtatataag tgatataaaa gagggaaaag atccatatgc
      781 tggtggtttc tggtttaatc tttcaaagat ttttaagggt attggagaaa cacttacaac
      841 tacaggagaa gcttctgaga gaaaagaggt tgttaaatta caacctgggc aggaggagga
      901 gttgagtgaa atagagttaa agatgcttaa agtcggtttt gagtttaaga ttagggtagt
      961 tactaaagct tctacacaga tagaatctga acaaatattg agggatattg ttgcaagttt
     1021 taagcagttt actactgctc atcttaattc ttttgtacat tcaaggccta aggagagtgg
     1081 tgagcagatt tattctaaat atgtaaagag atacttacca tctgatactg cagatattat
     1141 taatattgaa gagcttgctt ccttgtatca tatgcctaat atttctgttg aaacgccaaa
     1201 cattgcatgg agtaggtcaa agaaaataga accacctatg aatcttccca aagcaggaga
     1261 tgataatgtt actgtttttg cagagacaga ttatagagga acaaggtatg agtttggtat
     1321 aaaaaaggaa gatagaagaa agcattttta tatattaggt aaaactggag ttggtaaatc
     1381 tactgtattt aaaaatatgt ttatctcaga tatattgcat ggtgatggtg catgtatggt
     1441 tgacccccat ggagagcttg tagatgagct tttagatttt attcctccta atcgtataga
     1501 tgatgttata tatcttaatc ctactgatac acagtatcct attggtttta atcttcttga
     1561 gctaaaagac aaatcacaga gagatttaat agcggatgga gtggttgaag tatttcataa
     1621 acagtttggt acctcatggg gtcctaggtt gcagtatatt ttaacaaatg ctgttgctac
     1681 tgctcttgaa gcacaaggta caactctttt gtctgttaca aggcttctta ctgatagaaa
     1741 ctatcgtaaa tttatcttaa agcaggttaa tgatcccatt cttattaaat tctgggagga
     1801 agagtatgca caaatggccc agaatccaag acttttagca gagtctgtct ctcctataca
     1861 gaataaagtg ggtagattta tatcttcggc cgttactaga aacattattg gtcaggttaa
     1921 atcaacaatt gatcttagag atattatgga taacaagaaa atacttcttg ttaatttagc
     1981 acaaggaaaa cttggtgaag agactgcttc tttgctcggc ggaatgatta ttacaagatt
     2041 gcaatctact gcacttgaaa gagtagatat acctgctgat caaaggaatg atttctttct
     2101 ttttgtagat gaattccaaa attttgcaac agactcgttt gcaaaaatac tatctgaggc
     2161 aagaaaattt aggcttgatc tttgtatgac taatcagtat attgatcaga ttccattaac
     2221 tgtaaggcag gctatctttg gtaatgtagg tactttggga tcatttgttg taagtcaggc
     2281 tgatgcagca atattggaaa aagagtttgc tccttctgtt acttccgagg atttagtttc
     2341 acttgatgct cattctatgt atataaagct gtgtatagat ggaatgacat caaatgcatt
     2401 tagtgcaagg tctctaccgc ttagatatga accatttggc ttaagagagc aggtaattgc
     2461 tcaatctagg gagaaatatg gaactcctaa agaagttatt gaagacaaaa ttgcaagatg
     2521 gagcagtcag acatatagcg atagtggaaa caggtctttg agaaggaaag atgaaaacag
     2581 taataatcag aaagaatagt cccctttttc ttaatctgtc aaagtgatag aatatcttgt
     2641 atataacata tttttgtata tttatgtctg gacattctaa atggtcaaca ataaaacata
     2701 aaaaggagtt aaatgatgct aaaaagggaa aggtcttttc taagctgtca gtagaaatta
     2761 ctcatgcagc gaggaaagga ggaggggatc ctgaaatgaa tcctacactt agattgtata
//
LOCUS       DAOSAB010000070         2809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_23857_length_2809_cov_3.246187, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000070 DAOSAB010000000
VERSION     DAOSAB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23857_length_2809_cov_3.246187"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /gene="holA"
                     /locus_tag="PK432_01790"
     CDS             <1..674
                     /gene="holA"
                     /locus_tag="PK432_01790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOK59689.1"
                     /translation="TADNIIIWEDQKISSVTKYVKFFKTTNSFVEVNKMYKPAFIKFA
                     NELSKEYGLDMNREVVTLLCTYSNYDTQRLENNIKKLYLLELNKKIEKEDVRNVIADT
                     LEDDIWKLLDEMNKTKGQPLKILERILSQRVDPKYIFPMIARNMRLITMTKYLLDHHY
                     KYSEIASIVGVPPFTVKTLVEASDKYDWDKIMSKYEKLCNLDYEIKIGRIDPKLGLTL
                     FCTTV"
     gene            752..2506
                     /locus_tag="PK432_01795"
     CDS             752..2506
                     /locus_tag="PK432_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59690.1"
                     /translation="MEPTDRKTKTTLKNLMIIGAVLIVVLIVVLVYFFVIKEDETETP
                     QPITQTTCGCYIIDPAVVNDCGDPKRAFIFNLNTVSADQVCNAKCDINDIADNLLNST
                     TPKESLKTCPVRSISDTRCENLILRDQDGKIITGRVKPTDEINAEATFDKSTYVDYTF
                     KVNSEDSTPDKVDGNKITKKITPPPAVTAIEIVATAKDSQGEQINSMVCRRIIDVETS
                     GATAVTGMTAITEQQSDGKTKISQISISVGQLNSENIKIRFSFEPTFTTLTAIDGIIV
                     DPTKGTIEMSKLDLYDPNNFSNDSFNILNDHLGELNITAEVFVNESSIGTASTTVTFI
                     DTSPGEEPQPEEPQPLPDSEKSNFSTTKAVGQSCVQRTEGTNSATYTITVKNNRDVED
                     QIASVKDKLPLGFVYTTNSSIVNGTAIVDIDMVKITTVGDTQEIIWEPSAPWSIPAGG
                     TLTIVFQATAGPKALTGQNLNEVIVNPVQIPLDPSTLRSQVYIVVAQDCANITDEEKE
                     IPKTGIFDSFIGRIAVGIFIIIIGWIIYVRPEGTLLTEKIVTSKAYDDFEFRKSKITN
                     PKKYFEEKVLRKSSKGNR"
     gene            complement(2490..>2809)
                     /locus_tag="PK432_01800"
     CDS             complement(2490..>2809)
                     /locus_tag="PK432_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOK59691.1"
                     /translation="SASQDSVYVEGCTPYNIEIEKGKSPSSVVISWKSKEKCSAYILY
                     GKEVGGLDMVAVDLENEIKSNEHRVTIQNLVSTKKYYFVIVSNDVIYGKDGLPLQFSI
                     DSL"
BASE COUNT         1135 a          455 c          464 g          755 t
ORIGIN      
        1 atacagcgga taacataata atatgggaag atcaaaagat aagctctgtt acaaaatatg
       61 taaaattctt taaaacaaca aattcttttg tagaagtaaa taagatgtat aaaccagcat
      121 ttataaaatt tgcgaatgaa ctatctaagg agtatggatt agatatgaac agagaagttg
      181 ttactcttct ttgtacatat tccaactatg atacacaaag attagaaaat aatattaaaa
      241 aactctactt actggaatta aataaaaaaa tagaaaaaga agatgtgaga aatgtaatag
      301 ctgatactct cgaagatgat atatggaaac tgcttgatga gatgaataaa actaaaggac
      361 aaccattaaa aattttagaa agaatattaa gtcaaagagt tgatccaaaa tatatatttc
      421 ctatgatagc aagaaacatg agattaatta ctatgactaa atatctccta gatcaccact
      481 acaaatattc agaaatagct tcaatagtcg gggttcctcc atttacagtt aaaacactag
      541 ttgaagcaag tgataaatat gattgggaca agattatgag taaatatgag aaactctgta
      601 atctggatta tgaaataaaa ataggaagaa tagaccccaa gcttgggctt actctatttt
      661 gtacaacagt ataaggaaac ttgttgacat gatatatata ttctcataaa ctttatatac
      721 caacgtatat caatacgtta agaaacttta aatggaacct acagacagga aaactaaaac
      781 aacattaaaa aatttaatga taataggagc agttctcatc gtagtattaa tcgtagtgct
      841 agtatacttc tttgtaatta aggaggatga aacagaaact ccacagccaa taacccagac
      901 gacatgtgga tgttacataa tagatcccgc agtagtaaat gattgtggtg accctaaaag
      961 agcattcatc ttcaacctaa atactgtttc agcagatcag gtatgtaatg caaaatgtga
     1021 tattaatgat atagcagata atttacttaa ctctacaaca cctaaagaat cactaaaaac
     1081 atgtcctgtg agaagcatat cagatacaag atgtgagaat ctcatactta gagatcaaga
     1141 tgggaaaata ataacaggga gagttaaacc tacagatgaa ataaatgcag aagcaacttt
     1201 tgataaatca acctatgtag attatacctt taaagtaaat agtgaagata gcactcctga
     1261 caaagttgat ggaaataaaa taacaaagaa aattacacct ccaccagctg ttaccgccat
     1321 tgagatagtt gcaacagcaa aagattctca aggtgaacaa ataaattcaa tggtatgtag
     1381 aagaattata gatgttgaaa caagtggggc aacagcagta actggaatga cagctataac
     1441 agaacagcaa tctgatggga aaacaaagat atctcaaata agcatatctg ttggtcaact
     1501 taatagtgaa aatataaaga ttagattctc atttgaaccc acatttacaa cattaactgc
     1561 aatagatgga ataatagtag atcctacaaa aggcacaata gagatgtcaa aacttgactt
     1621 gtatgatcca aacaatttta gcaatgattc atttaatatt ctcaacgatc atttaggaga
     1681 gcttaacata acagcagagg tatttgtaaa tgaatctagt atagggactg caagtacaac
     1741 tgtaacattt atagatacct caccaggaga agaaccacaa ccagaagaac cacaacctct
     1801 tcctgactca gaaaagtcta actttagtac aacaaaagcg gtaggacaat cgtgtgtaca
     1861 aaggacagaa ggcacaaact ctgcaacata tacaattact gtaaaaaata acagagatgt
     1921 agaagatcaa atagcttctg taaaagacaa attacctcta gggtttgtgt acacaactaa
     1981 tagctcaata gtaaatggaa cagctattgt agatattgat atggtaaaaa ttacaaccgt
     2041 aggtgataca caagagataa tctgggaacc ttctgcacca tggagcatac cagcaggagg
     2101 aacattaact atagtattcc aagcaacagc aggacctaaa gcattaacag gacaaaactt
     2161 aaatgaggta attgtaaacc ctgtccaaat accactagat ccttctactc tacgatctca
     2221 agtatacata gttgtagcac aagattgtgc taatattacc gatgaagaaa aagaaatacc
     2281 gaagacagga atatttgaca gtttcattgg aagaattgca gtcggaatat tcataattat
     2341 aatcggatgg ataatatatg taagaccaga aggaacactc cttaccgaaa aaattgtcac
     2401 ctcaaaagca tacgatgact ttgagtttag aaaaagtaaa ataacaaatc cgaagaaata
     2461 ctttgaagaa aaagtcttaa ggaaaagctc taaagggaat cgatagaaaa ctgtaaagga
     2521 agtccatctt taccataaat cacatcattt gaaactataa caaaatagta ttttttagta
     2581 cttaccaagt tttgaatagt taccctatgc tcattgctct ttatttcgtt ttctaaatct
     2641 actgcaacca tatctaatcc ccctacttcc ttaccataca aaatatatgc tgaacacttc
     2701 tctttgcttt tccatgagat aacaacagag cttggagact tacctttctc aatctcaatg
     2761 ttgtaagggg tacatccttc tacatataca gagtcttgcg aagcagatg
//
LOCUS       DAOSAB010000071         2805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_23918_length_2805_cov_2.852727, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000071 DAOSAB010000000
VERSION     DAOSAB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23918_length_2805_cov_2.852727"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..294
                     /locus_tag="PK432_01805"
     CDS             70..294
                     /locus_tag="PK432_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOK59692.1"
                     /translation="MNPIKDTVKDITSMLTFNGIALILFAVLIFIFPDLLSYLVGAFL
                     CIAGISSLVYGFRMNKSYKDAEKFWKKFEE"
     gene            complement(330..1127)
                     /locus_tag="PK432_01810"
     CDS             complement(330..1127)
                     /locus_tag="PK432_01810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK59693.1"
                     /translation="MPKHCVSAIVPAFNEEKTVFNVISILQSSSLINEVICINDGSSD
                     RTKEILERFKNDKKVKVINLDKNKGKGNALAVGIKHSKGDILLFIDADLINLTKDHIY
                     SLLEPLLQGTHRAVLGYISGKGNRNIVADLTGQRAYFKKDLEPYIDEISKTRFGVETY
                     LNKLFKAEDTIKVPLHNLKGLFKYEKQRADIALKEYLKEGVEIAKVIGNTEIISDEDM
                     KVLQKILNISDITTLKKYLNKISNLKIKNSLQKYFNQYVDIIRKIYP"
     gene            complement(1164..1865)
                     /locus_tag="PK432_01815"
     CDS             complement(1164..1865)
                     /locus_tag="PK432_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59694.1"
                     /translation="MIGEQTEQPFSATEETLKTAKESGLEDIIKEVSNEQNIYDSNTL
                     FDIPIPLELIEEDKTPITVNESLKGPLNELLDIIKDKEKGKEYPFLLTGSSKDEDGMI
                     KDMKFTFNIQYLKNNYAEINPELLQEGIQNGVNNNYDTYIICHTHPNIQDEEKQKMLA
                     SKISPKLKEKFGIKEAGLNLSLQDLRQLVAFNHRLKDRLPPNSTVYMAVLMHNGAFEA
                     VHIENGKFKRKRIEI"
     gene            1959..2621
                     /locus_tag="PK432_01820"
     CDS             1959..2621
                     /locus_tag="PK432_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK59695.1"
                     /translation="MSYIQVQNLKRRYAISKSVSVDALRGVTFDVEKGEFISVIGSSG
                     SGKSTLLNILGGLDRKYTGSVYVDEKDIKEYDPNYYRRYIVGTIFQQFYLIPSLNVEE
                     NILLPLKFGKKEGIDFEERLNYLLDIVGLKHRVKHFPRELSGGEAQRVAIARALIDSP
                     KIVLADEPTGNLDSKTGNDILELLKELNKEEELTILLVTHDQAIAQKTDRIITLVDGK
                     NV"
BASE COUNT          873 a          393 c          421 g         1118 t
ORIGIN      
        1 agaaagagtt tgtaccatta gaggaataat atataatata cttatattta atttgtttta
       61 tttaaacata tgaatcctat taaagataca gtaaaagata taacctccat gcttacattt
      121 aatggtatag cattaatact ttttgcagta ctcatattta tatttcctga tcttttaagt
      181 tatttagttg gggcatttct ttgtattgca ggtattagtt cattagtcta tggatttaga
      241 atgaataaat catacaagga tgcagaaaag ttctggaaga agtttgaaga gtaagagctg
      301 ataaaaagat aataaaacag catactatcc tatggatata tctttctaat tatatctaca
      361 tactgattaa aatatttttg cagactattc ttaatcttta agttagatat tttattcaga
      421 tatttcttaa gggtagttat atcactaata tttagaatct tctgtaatac tttcatatct
      481 tcatctgata ttatttctgt attacctatt acctttgcta tttcaacacc ttcttttaaa
      541 tattctttta atgcaatatc agctctttgt ttttcatatt taaataatcc ttttaaatta
      601 tgcagaggaa cttttattgt atcttcagct ttaaataatt tatttaaata tgtttctaca
      661 ccaaacctgg tttttgatat ttcatctatg tatggctcca aatctttttt aaagtaagct
      721 ctttgacctg ttaaatcagc aacaatattt ctatttcctt ttcctgatat atatccaagt
      781 acagctctat gagttccttg tagtaatggt tctagaaggg agtatatatg atcttttgtt
      841 aggtttataa ggtctgcatc aataaaaagg agtatgtctc ctttagaatg tttaattcca
      901 actgctagag catttccttt tcctttgttt ttatctaagt ttattacctt aaccttttta
      961 tcatttttaa acctttctaa tatttccttt gttctatcac tagagccatc attaatacat
     1021 ataacttcat tgattagaga tgaggattgt aatatcgata ttacattgaa tacagttttt
     1081 tcttcattaa aggcgggtac tattgctgat acacagtgtt ttggcatagt tttagtttat
     1141 ttagagctaa acacatgatt atatcaaatt tctatccttt ttcttttaaa ctttccattt
     1201 tcaatatgta ctgcctcaaa tgctccattg tgcattaaaa cagccatata tactgttgaa
     1261 ttgggaggta gtctatcttt taacctgtga ttaaaagcaa ctaattgcct taggtcttgt
     1321 aatgaaagat ttaatcctgc ttcttttatt ccgaacttct ctttgagttt aggtgatatt
     1381 ttacttgcta acatcttctg tttttcttcg tcttgtatat taggatgtgt atgacagata
     1441 atatatgtat cataattatt atttactcca ttttgaatac cctcttgtag tagttcagga
     1501 ttaatttcgg catagttatt tttaagatat tgtatattga atgtaaattt catatcttta
     1561 atcattccat cttcatcttt tgaagagcct gttaaaagaa atggatattc ctttcctttt
     1621 tccttatctt ttattatatc caatagctca tttaatggtc ccttcaagct ttcatttaca
     1681 gtaattggtg tcttatcttc ctcaattaat tcaagaggaa tagggatatc aaaaagtgtg
     1741 tttgaatcat aaatgttttg ctcatttgaa acttctttta taatatcttc aagaccactt
     1801 tcttttgctg ttttgagtgt ttcttctgtt gcactaaatg gttgttccgt ttgttctcca
     1861 atcatatttt attatattga ttgtttagta ttatttaaag gcatctttac tcatttgaat
     1921 tattatatat tttaatgtaa tattatgtct tcgcaattat gtcatatata caagtacaaa
     1981 atctaaaaag aagatatgct attagtaaaa gtgtctctgt agatgcttta agaggtgtta
     2041 cctttgatgt agaaaaaggt gaatttatct ctgttatagg tagctcaggt tcagggaaat
     2101 ctactttgtt aaatatctta ggtggattag atagaaagta tactggtagt gtatatgtag
     2161 atgaaaaaga tatcaaagaa tatgatccta attattatag aagatatatt gttggaacaa
     2221 tatttcagca gttttacctt attccatccc ttaatgtaga ggagaatatc cttcttccat
     2281 taaaatttgg aaagaaagag gggattgatt ttgaagaaag attaaactat ctcttagata
     2341 ttgttggact taaacatagg gtaaagcatt ttcccagaga gctttctgga ggtgaggcac
     2401 aaagagttgc aatagctagg gcattaatag attctcctaa gatagtactt gctgatgagc
     2461 caacaggtaa tttagacagt aaaacaggta atgatatatt ggaacttcta aaggagctaa
     2521 ataaagagga ggagcttact attttacttg ttactcatga tcaggcaatt gctcaaaaaa
     2581 cagatagaat tattacttta gtagatggta aaaatgttta aatatgcagt aagccttata
     2641 tcaaggagaa aacttagaac atttctgaca agtcttggaa ttatgatagc ggttatgctt
     2701 atgacattca tactttttgg tatgacagat ttgcaggatg ctgttcttac ccagtttaat
     2761 tctgtcttta aacctacaga tctttatgta tcaaacagca gcttt
//
LOCUS       DAOSAB010000072         2733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_24808_length_2733_cov_2.594100, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000072 DAOSAB010000000
VERSION     DAOSAB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24808_length_2733_cov_2.594100"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK432_01825"
     CDS             complement(<1..369)
                     /locus_tag="PK432_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOK59696.1"
                     /translation="MRVYLKEIKEKFPEFEIENYDENIYFENINHDSRVYIADSLFVP
                     IIGKNFDGHDFIESSKCSVSLMQRNHKGRYKLDIPLIIVDDILEGLKEIIEYVRDKVN
                     TPVIGITGSTGKTTTRMMSVS"
     gene            538..1011
                     /gene="mraZ"
                     /locus_tag="PK432_01830"
     CDS             538..1011
                     /gene="mraZ"
                     /locus_tag="PK432_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOK59697.1"
                     /translation="MARSGKKWYIQNMIIGEYTSKIGEKKRVSLPKKFRDEIGEDIIL
                     TRGYEDSLILVNKQMWEKIAKDVINGSFINKNIRDTSRFLIGSATEISLDKLGRFVIP
                     QSLFEHAQLDNEIVFIGLINWIEIWDKSRWEKRVEYLKAHGEEIAQEIGRMKEGN"
     gene            1012..1899
                     /gene="rsmH"
                     /locus_tag="PK432_01835"
     CDS             1012..1899
                     /gene="rsmH"
                     /locus_tag="PK432_01835"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOK59698.1"
                     /translation="MKKLHTPVLLNELILYLNIKPDGFYIDCTLGDGGHSFEIFKRLD
                     KNGLLISIDQDKYAIDFVKEYYKKDIKGNWIIVKDNFANIDKIANQYNRRPDGILMDL
                     GLSSRQLEDSHNRGFSYLEDKEALDMRMDEELGVTAKDLLTALSENELTEIFLKYGEE
                     KYARKIAKAIKKENTNINTVGDLTRLVYRVVPAAHQSSKNPSRRVFQALRIVVNDEIN
                     SLKIGIDKSFKLLNKKGRLCVISFHSLEDRIVKNYFNNLVESGEGMLLTKEIIAPTDQ
                     EVRDNPRSASAKLRAIEKI"
     gene            1919..2221
                     /locus_tag="PK432_01840"
     CDS             1919..2221
                     /locus_tag="PK432_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59699.1"
                     /translation="MRKTEKKNSLFSMLLVASFVLFVVSQLVINSILTPLGIKLEELN
                     DEKEYLLEENRVISEEIAKNSSLKVIENLSEKKLSLSKNISQTFIYIQDSRLVANN"
     gene            2221..>2733
                     /locus_tag="PK432_01845"
     CDS             2221..>2733
                     /locus_tag="PK432_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59700.1"
                     /translation="MTSLSRLHSPKYKKNSSETGALVQRNNYKLVAVLLTIFGIIILI
                     QAFRWQVLESDKFASIAQQQYTNTGKQTAQRGRVLAEDGTILAVDQPAWNVYASLSTD
                     KRERELFFSKKDLFVSTVASILAVETETIKEKLTDDFVYVNLAKEVDNETKIALASAN
                     IFGNENINRAG"
BASE COUNT         1053 a          386 c          455 g          839 t
ORIGIN      
        1 agaaacactc atcatccttg ttgtagtttt tcctgtactt cctgtaatcc caataactgg
       61 agtattaact ttatctctta catattcaat aatttctttt aacccttcta aaatatcatc
      121 tacaattatt aatggaatat ctagtttata cctcccttta tgatttcttt gcattaaaga
      181 aacactacat ttacttgatt caataaaatc atggccatca aaatttttcc ctattatcgg
      241 aacaaacaag gagtctgcaa tataaaccct tgagtcatga ttaatatttt caaaatatat
      301 attctcatca taattttcta tttcaaactc aggaaacttt tcttttatct cttttaaata
      361 aactctcatg tttcatttta ctatatatct acaccttttg ttacctttat cttttactat
      421 cagttatctt taatttttta tctctatttt ttcttcatca ttttaagata aattcttaat
      481 aacatatgtc ttgtagtatt attctcttga ctactattaa tattcaatgc tacattagtg
      541 gcaagaagtg gtaaaaagtg gtatattcaa aatatgataa ttggagaata cacaagcaaa
      601 ataggggaga agaaaagagt atcacttcct aaaaaattta gagatgaaat aggggaagac
      661 ataatattaa caagaggata tgaagattct ttgatacttg ttaataaaca gatgtgggag
      721 aaaatagcaa aagatgttat aaatggctct tttataaata aaaatattcg tgatacaagc
      781 agattcctaa taggtagtgc tacagaaatt tcattagata agctgggaag atttgtaata
      841 cctcaatctc tttttgaaca tgcacagctt gataatgaaa ttgtcttcat tggtttaata
      901 aattggattg agatatggga taaatcaaga tgggagaaaa gagtggagta tcttaaagca
      961 catggagaag agattgctca agagatagga agaatgaagg agggtaacta aatgaagaag
     1021 ttacataccc cagtactgct caatgagtta atactttacc taaatattaa accagacgga
     1081 ttttacatag attgtacatt aggagatgga ggacacagtt ttgaaatatt taaaagacta
     1141 gacaaaaatg gattgttaat tagtatagat caagataaat atgcaattga ttttgtaaaa
     1201 gagtattaca aaaaagatat aaagggaaac tggataatag taaaggacaa ttttgcaaat
     1261 atagataaga tagcaaatca gtacaatcgc agaccagatg ggatactaat ggatttaggt
     1321 cttagctcaa gacaattaga agattcacat aatagaggtt ttagctatct agaagataaa
     1381 gaagcacttg atatgagaat ggatgaagaa cttggagtaa ctgctaaaga tctgcttact
     1441 gctctttcag agaatgaact tacagagata tttcttaagt atggagaaga aaaatatgca
     1501 agaaaaattg caaaagcaat taaaaaagaa aacacgaata ttaatactgt tggagatctt
     1561 acaagacttg tctatagagt tgtgccggca gctcatcaaa gttctaagaa cccttctcgc
     1621 agagtctttc aggctctgcg aattgtggta aatgatgaga taaactcatt aaaaataggc
     1681 atagacaaat cttttaagct cttaaacaaa aagggaaggt tatgtgtaat atccttccat
     1741 tcacttgaag atagaatagt taaaaattac tttaacaatt tagttgaaag cggagaaggt
     1801 atgcttctta caaaggaaat cattgcccca accgaccaag aggtaagaga taatccccga
     1861 tccgcttcag ctaagttaag agctatagaa aaaatataaa aatttaaaaa ctgcctaaat
     1921 gagaaagact gagaaaaaaa actcactttt ttcgatgcta ctagtagcct cttttgtcct
     1981 ttttgttgta tcacagcttg ttattaattc aatactcacc cctcttggaa taaaactaga
     2041 agaactaaat gatgaaaaag aatatctgct agaagagaat agggtaatat cagaggaaat
     2101 tgcaaaaaac agctcactta aagtgataga gaatctttct gagaaaaaat tgagtttatc
     2161 aaaaaatata agtcaaactt tcatatacat tcaagattct agattagttg ctaacaacta
     2221 atgacaagcc taagccgttt acattcacca aaatacaaaa aaaacagctc tgaaacagga
     2281 gcattagttc aaaggaataa ttataaacta gtagcagtac tcttaactat atttggaata
     2341 ataatcttaa tacaagcatt tagatggcag gtgcttgaat cagataaatt tgccagcatt
     2401 gcacaacaac agtatacaaa tactggaaag caaactgccc aaagaggaag agttttagca
     2461 gaagatggga caatacttgc agtagatcaa cctgcttgga atgtatatgc ttcactaagt
     2521 acagataaaa gagaaagaga attgtttttt agtaaaaaag atctttttgt ttctactgtt
     2581 gcctctattc tagctgttga aactgaaact ataaaagaga agttaacaga tgattttgta
     2641 tatgtaaatc ttgcgaagga agtagacaat gaaacaaaaa ttgcattagc aagtgcaaat
     2701 atatttggta atgaaaatat aaatagagct ggt
//
LOCUS       DAOSAB010000073         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_24869_length_2728_cov_2.012346, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000073 DAOSAB010000000
VERSION     DAOSAB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24869_length_2728_cov_2.012346"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2728
                     /locus_tag="PK432_01850"
     CDS             <1..>2728
                     /locus_tag="PK432_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59701.1"
                     /translation="DDILETNEKYKSGMTLKDYLKSEQFFKSLTEAGNIQNLFNTDSA
                     LKTAIDSQMNTWFNALREAYSKNIYNPVFGNVKTRFGTDKANTVFSDLIQKNLDANEK
                     ARVNSSSIREAGEWSYLVNNFIHKIETTVLLHGDGFQFNHSKDNSPKRASGAQSSGRV
                     FPVDAITNLWINNVVGRRLEEKLIADGLITKKEGKNEVRKHTPIMNSAIMQEPVMGSA
                     FFEMYAKLFKKDFENKGLEGDALKIALYGEKDGKIGTGKLKKDGTFDTYPGGKMHPYS
                     KIESADGQGWISFDSYRILKKLEGMWSDDQEAAYSRIVNGETLSPGELIELFPVYKLQ
                     YNGPLHTPGQYPAQAFHKFSLFPLIPGAIAGKPIEKISMAMMEQNIDYVLSPTGSKRS
                     YIKPSAEQNGDPVYTGNTSNVKNINEITFTKNPIYVAYLKNQTDVNSFFKEESTFSTQ
                     LRKLITAGLYEFGIPTDFYKEGKFKDPEDAILAWGKLTEAEKKQTSEYHRLSERLKDA
                     IEDLVEEAKIELLESIKWTEEDLNKTSKELAAESPEKLKELFGLINKELKEQDFSDHE
                     RSILEASSNLDKLDASSAPLAARFEKVIRSILNKRLIRLKLRGEPFVEVSSDYMQQTV
                     KFTKPSEEQQKAYDNFGNGTNGLSSYVVDIKGKKKTLGFRFKRALTRHDERLFNTYFY
                     DQKGSRVPIKNDKGVIIGYEKIGVFDEVDGQKKLNIKASLARLNQMINNNEWLEDDDN
                     KKKIRLVGVRIPVQADNSAEFGQVAEFLDPSAGTIIIIPAEIVPKSGTDFDVDKMTAY
                     LPYITRTGKLIEKKVSKKSLDDKITKAKEKIDAFKKNKEIIAEFKESKGVKWLDIGEK
                     LFEFREILKKEKKSKNIFLDKKTLEKLLYSKSKELVEYLNNNEEYVKRSFPKA"
BASE COUNT         1039 a          363 c          513 g          813 t
ORIGIN      
        1 ttgatgatat actagagact aatgagaagt ataagtcagg tatgacctta aaggattatc
       61 taaagagcga acaattcttt aaatcactta cagaagcagg taatatacaa aatttattta
      121 atacagactc tgcattaaag actgctatag attctcaaat gaatacttgg tttaatgctc
      181 ttagagaagc atattcaaag aacatttata atccagtatt tggtaatgtt aaaacaagat
      241 ttggtactga taaagctaat acagtattct cagatcttat tcaaaaaaat ctagatgcta
      301 atgaaaaagc tagagtaaac tcttcttcta taagagaagc tggtgaatgg tcttatttag
      361 taaataactt tatacataaa attgaaacca ctgttttatt acatggtgat ggcttccaat
      421 ttaatcattc taaagataac tctccaaaaa gagcgtctgg agctcagtct tcaggtaggg
      481 tattcccggt agatgctata acaaatcttt ggataaataa tgtagtagga agaagattag
      541 aagaaaaact tatagctgat ggtctaataa ccaagaaaga aggtaaaaat gaggttagaa
      601 aacatactcc tataatgaac tctgctatta tgcaagagcc ggtaatggga tctgccttct
      661 ttgaaatgta tgctaaactg tttaaaaaag actttgaaaa taagggctta gaaggtgatg
      721 ccttaaagat agctctttat ggagaaaaag atggtaaaat aggaacaggt aagttaaaga
      781 aagatggaac ttttgatact tatccaggtg gaaaaatgca tccttattct aaaatagagt
      841 ctgctgatgg tcaaggttgg ataagctttg atagttacag aatacttaag aagttagaag
      901 gtatgtggag tgatgatcaa gaagctgctt attcaagaat agttaatggt gaaacacttt
      961 caccaggtga gcttattgaa ttattccctg tatacaagct tcaatacaat ggtcctttac
     1021 atactccagg acaatatcct gcacaagcat ttcataagtt ctctttattc ccacttatac
     1081 caggtgctat agctggtaaa cctattgaga agattagcat ggcaatgatg gaacaaaaca
     1141 ttgattatgt tctatcacct accggatcta agagatctta tataaaacca agtgctgagc
     1201 aaaatggtga tcctgtatat acaggaaata ctagtaatgt aaaaaacatt aatgaaataa
     1261 catttactaa gaaccctatt tatgtagcct acttaaagaa ccaaacagat gtaaacagct
     1321 tctttaaaga agagtctaca ttctctacac agttaagaaa gcttatcaca gctggtctat
     1381 atgaatttgg tatacctact gatttctata aagagggtaa gtttaaagat ccagaagatg
     1441 ctatactagc atggggtaaa ctaactgagg cagaaaagaa acaaacaagt gaatatcata
     1501 gattatctga gagacttaaa gatgctatag aagatcttgt agaagaagct aaaatagagt
     1561 tacttgaatc tattaagtgg acagaagaag atcttaataa aacatctaaa gagctagctg
     1621 cagaatctcc tgaaaaacta aaggaacttt ttggtttaat caataaggaa ttaaaagaac
     1681 aagatttcag tgatcatgaa agatctatat tggaagcctc atccaattta gataagctgg
     1741 atgctagttc tgcacctttg gctgcaaggt ttgaaaaggt tatcagaagt attttaaata
     1801 aaagacttat tagattaaag cttagaggag aaccttttgt tgaggtatct tctgattata
     1861 tgcagcagac tgttaagttt actaaacctt ctgaagaaca gcaaaaagct tatgataact
     1921 ttggaaatgg tactaacggt ctttcttctt atgttgtaga tataaagggt aaaaagaaaa
     1981 ccttaggttt cagatttaag agagctctta ccagacatga tgagagatta tttaatactt
     2041 atttttatga tcagaaaggt tcaagagtac ctattaagaa tgataaagga gttattatag
     2101 gctatgaaaa aataggtgta tttgatgaag ttgatggtca gaaaaagtta aatatcaaag
     2161 ccagtcttgc tagattaaac cagatgatta ataataacga atggttagaa gatgatgaca
     2221 ataagaaaaa aataagatta gttggtgtaa ggattcctgt acaggctgat aactctgctg
     2281 agtttggtca ggtagctgaa ttcttagatc catccgcagg tactattatt attatcccgg
     2341 ctgagatagt acctaagtct ggtactgact ttgacgtgga taagatgact gcttacctac
     2401 catatattac aagaactggt aagcttatag aaaagaaagt ttctaaaaaa tctcttgatg
     2461 ataagataac aaaagctaaa gaaaagatag atgctttcaa gaaaaacaaa gaaattattg
     2521 cagagtttaa agaatctaaa ggtgttaagt ggcttgatat tggtgagaag ttatttgagt
     2581 ttagagagat tctgaaaaaa gaaaaaaaat ctaaaaatat atttttagat aaaaaaactc
     2641 ttgaaaaact tctttattct aagagtaaag aacttgttga gtatcttaat aataatgagg
     2701 aatatgtaaa aagatctttt cccaaagc
//
LOCUS       DAOSAB010000074         2717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_24992_length_2717_cov_4.225770, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000074 DAOSAB010000000
VERSION     DAOSAB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24992_length_2717_cov_4.225770"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /gene="mltG"
                     /locus_tag="PK432_01855"
     CDS             <1..1009
                     /gene="mltG"
                     /locus_tag="PK432_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOK59702.1"
                     /translation="KVKMKSLIKIIILAVITVIAITVLAKVNFNSVIKKPNSESSEKV
                     TLQIEPGESVDSIINKLVDAGILKENWKNHFKYYLKINDLSESIQAGVYEIPKNLNIK
                     EIAQTLQQARGLDLWITIPEGLRKDEIAQLLAMELAKGGNTNFNSDEFLKLTTDPSFI
                     STLGFNYELSDLEGFLFPDKYAFSVKETTQDILKKLTDNFQAKVGINDSYEDIIIASM
                     VEREGYNAQDRAMIADIIQRRYKEGWLLQIDATLLYPLKDWKHVITQEDKNSNSPYNT
                     YKYPGLPPTPICNPGLESINAVRNPQSNDYYYYIHDNEGNIYYAKNLTEHNQNIEKYL
                     K"
     gene            1031..2476
                     /locus_tag="PK432_01860"
     CDS             1031..2476
                     /locus_tag="PK432_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOK59703.1"
                     /translation="MEVSINLTDKEINNGKPLKERSVQTHTKKKRPFLTILFIFLVLV
                     IGSGLYFGYKGYMTGKGIGLNLSPSDLIGKQKEPELKKDSTGAYTNILIVGIDTRTKL
                     KTFNTDVIIVASYNHKTKDVVMISIPRDFCTKVNPSKTSYGKINSAYSIYEAKEKGSG
                     LEGLKGIVEEITGMEIQYYTMINFNGFVELVDAVGGLYINVENSFTDYKYPAETGYGT
                     QTISFKAGPQLMDGDTALKYARSRQSQQNGEGSDYARAKRQHKVIDAFIKKLLSTETL
                     LNPNKVMSLLTSIQDNIKVSEFTIDDIQAGVNVLKSLSSESSTSSTYSFVLDPTIGNY
                     SLISHQPSCGEYNIGPKEGLGRYTKLGEYINLALSYPALYSENPSIYSYDTGLGYQNT
                     KTKTEELKTRFKYLDIRFQGTKFNDKEGTYIYSNTTDKFSKSVDILASYLKTNNKVKP
                     EYLTTATGGDISILFGKEIIQDETVEIQQSE"
     gene            2476..>2717
                     /locus_tag="PK432_01865"
     CDS             2476..>2717
                     /locus_tag="PK432_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59704.1"
                     /translation="MYKLYIGNETYLSLKRAQEDIRELLKNGNLEYISLDAEETESSK
                     IIELLSSQSLFNIPRLIFLKRLYKNKDRENLIPYIL"
BASE COUNT         1103 a          392 c          459 g          763 t
ORIGIN      
        1 caaagtaaaa atgaaaagct taattaaaat aataatatta gcagtaatta ctgtaatagc
       61 tataacagtt ttagcaaaag taaactttaa ctctgttatt aaaaaaccaa atagtgaaag
      121 tagtgagaaa gttacattac agatagaacc aggcgaatct gttgattcta taattaataa
      181 attagttgat gcaggaatat taaaagagaa ttggaaaaac cactttaagt attatcttaa
      241 aataaatgat ttatcagaaa gcattcaggc aggagtatat gaaattccta agaatttgaa
      301 tataaaggag attgcacaaa ctcttcaaca agcaagaggt ctagacctat ggataacaat
      361 tccagagggg cttaggaaag atgaaattgc acaactactt gcaatggagc ttgcaaaggg
      421 aggaaataca aactttaact ctgatgagtt tttaaaactt acaactgatc cttcttttat
      481 ttcaactctc ggttttaatt atgagctttc tgatttagaa ggttttcttt tcccagataa
      541 atatgcattt tctgtaaaag aaacaacgca ggatatacta aagaagctga cagataactt
      601 ccaagcaaaa gtcggtatta atgacagtta tgaggatata atcatagcaa gtatggttga
      661 aagagaagga tataatgcac aggacagagc aatgattgct gacataatac aaaggagata
      721 caaagaagga tggcttcttc aaatagatgc aacacttctt tacccactaa aagattggaa
      781 gcatgtaatt actcaagaag ataaaaatag taacagccca tataatacat ataaatatcc
      841 tggtcttccc cctaccccaa tatgcaatcc tggtctggaa tcaattaatg ctgtaagaaa
      901 cccgcaatca aatgactact attattacat tcatgataat gaaggaaaca tatattatgc
      961 gaagaattta acagagcata atcaaaatat cgagaaatat ttgaaatagc taagttctaa
     1021 tatatatttt gtggaagtat ctataaatct tacagacaaa gagataaaca atgggaagcc
     1081 tttaaaagaa aggtcggtac aaacacatac caaaaagaaa agaccctttt taacaattct
     1141 tttcattttc ttggttcttg taattgggag cggattgtat tttggatata aagggtatat
     1201 gacaggaaaa ggaataggcc ttaatctttc tcctagtgat ttaattggta aacaaaaaga
     1261 accagagtta aagaaagata gcactggagc atatactaat atattaattg taggtataga
     1321 tacaagaaca aagctgaaaa catttaatac agatgtaatt attgttgcat catacaatca
     1381 taaaactaaa gatgttgtaa tgatatctat accaagagat ttctgtacaa aagtaaaccc
     1441 aagtaaaaca tcgtatggaa agattaattc tgcatattcc atttatgaag cgaaagaaaa
     1501 aggtagtggt ttagaggggt taaaaggtat tgtagaagaa attacaggaa tggaaattca
     1561 atactataca atgattaact ttaatggttt tgtagaacta gttgatgcag taggaggact
     1621 atacattaat gtcgaaaata gttttacaga ttataaatac cctgcggaaa caggatatgg
     1681 tacacagaca atatccttta aagcaggtcc ccagcttatg gatggagata ctgcgcttaa
     1741 atatgcaagg tcaagacaat ctcaacagaa tggagaagga tcagattatg caagagcaaa
     1801 aagacagcat aaagtaattg atgcttttat taaaaagctt ctaagcacag aaacactcct
     1861 taatcctaat aaagttatgt ccctcctaac ttccattcaa gataatataa aggtatcaga
     1921 gtttacaatt gatgatatac aggcaggagt taatgtacta aaatctctat ctagtgaaag
     1981 ctctacatct tcaacatact catttgtact tgatccaaca attggaaatt actctttaat
     2041 tagtcaccag cccagctgtg gggagtataa cataggacca aaagaggggt tgggaagata
     2101 tacaaagctt ggagagtata taaacctagc attaagctac cctgcccttt attcagagaa
     2161 tccatctata tattcatatg acacaggatt gggatatcaa aatacaaaaa caaaaacaga
     2221 agagttaaaa acaaggttta aatacttaga tattagattc cagggaacaa agtttaatga
     2281 taaagaagga acatatatat actcaaatac aacagataaa ttctctaaat cagttgatat
     2341 actagcaagc tatctaaaaa caaacaataa agttaaacca gaatatttaa ctacagcaac
     2401 aggaggtgat atctctattc tttttggaaa agagataata caagatgaaa ctgtagagat
     2461 tcaacaaagt gaataatgta caaactatat ataggaaatg aaacatatct ttctctgaaa
     2521 agggctcaag aagatattag agagcttctt aaaaacggaa acttagaata tatatcactt
     2581 gatgcagaag aaactgaaag ctctaagata atagagttac tttcttctca atctctattt
     2641 aacataccaa gattaatatt ccttaaaagg ctttataaaa ataaagacag agaaaattta
     2701 attccatata tacttga
//
LOCUS       DAOSAB010000075         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_25590_length_2672_cov_4.153611, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000075 DAOSAB010000000
VERSION     DAOSAB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25590_length_2672_cov_4.153611"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2475)
                     /locus_tag="PK432_01870"
     CDS             complement(<1..2475)
                     /locus_tag="PK432_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59705.1"
                     /translation="MANNFQNTSIVKTNTFSKGMFKDTNDMYMSEGLWNHAVNAINNT
                     HYGEVGTIGNEPSNKECASAPYTIIGYAYKSNGQWVLFSTRQGTHGSIPDEPNSDIGI
                     FDEATCTYTSIFNSNANTMCLDFAVVSPITAVCKENYDCTWSVYWQDGHNPDRVINLD
                     KPAYIPNPNSDDDPCTDEGVSATLDCDAIRLHPLVSQPCLKIRKSKGGGQLPNGSYMA
                     VIAYSENGIRLTDYSMPSPAVGLWSHEGIGGSIEVLVNNTDENFNEFELVVIAVVNQQ
                     TIAKKIGHYPINIETIHLDQILQSLPTVDLSTIPMKNMVYEKSDKMFEINHHLVRCGV
                     TSQPYFNYQPLANKIKSKWVAVEYPKEYYFSGGTSVGYMRDEVYSFFIRWIYKTGARS
                     ASYHIPGRAPNATDRNSVVGDPNRVYTTETERWQVYDTSTESNNLIGQTINDSIEGVE
                     GVIVKSGEMSYWESTEKYPTNPEIWGDLCNKPIRHHKMPSDETIAISRKDNDGKEYII
                     VLGVKFENILPPVDTDGNIIKDIVGYEILRGSREGNKSIIAKGLFNNMLEYKIPDITQ
                     KGLVQNYPYNDVREDPFLAVDATNMTNSNRRPELIQPDSFKKDIFSFHSPETNFIKPY
                     IGNSYIKLYREVSGYAKGKFSIPYKHPQHKLITTSAFALAIPVAVGIGIVAGLGKTTF
                     NAGVEAGVLVASADAHAIRDAGSATAIPDLIANMGYQGTSVLGVGGVVATAAGIVIMG
                     MNFTYYFGQGMNQVLNIIRNISKWRNYLLQYNSHGLYDKWHEISRAASGAPDDVKRSI
                     RRPSKTVKYIGSHVQDLNSNYRINNIN"
     gene            complement(2478..>2672)
                     /locus_tag="PK432_01875"
     CDS             complement(2478..>2672)
                     /locus_tag="PK432_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59706.1"
                     /translation="RICENLIANGEPLQNLFNLMNIQLRKAKIDAISFVRTPNFKEMH
                     KAWQMNRKAMYMKYYDMFKG"
BASE COUNT          862 a          468 c          342 g         1000 t
ORIGIN      
        1 atttatatta tttattctat aatttgagtt gagatcttgt acatgagaac ctatatattt
       61 tacagttttg gaaggtcgcc taatacttct tttaacatca tcaggtgctc cagatgctgc
      121 acgggatatt tcatgccatt tatcatataa accatgacta ttatattgta ataaataatt
      181 tctccatttg cttatgtttc tgattatatt caatacttga ttcattcctt gaccaaagta
      241 ataagtaaaa ttcataccca taattacaat gccagctgct gtagctacta caccgccaac
      301 acccaataca gaagttcctt ggtaacccat attagcaatt aaatctggaa ttgctgtagc
      361 tgagccagca tctctaatag cgtgagcatc agcgcttgct actaatactc cagcttctac
      421 accagcatta aatgtagttt tacctaatcc tgctacaata cctataccaa ctgctacagg
      481 aattgctaat gcaaaagcac ttgtagtaat caatttatgt tgtgggtgtt tataaggtat
      541 tgaaaattta cctttagcat atcctgatac ttctctatat aatttgatat aactatttcc
      601 tatataaggt ttaataaaat tagtttcagg agaatgaaat gaaaatatat cttttttaaa
      661 gctatcaggt tgaattaatt caggtcgtct attagagtta gtcatatttg tagcatctac
      721 tgctaaaaac ggatcttctc taacatcatt gtaaggataa ttttgaacta aacccttttg
      781 agtaatatca ggtatcttat attcaagcat attgttgaat aatccttttg cgataatact
      841 tttattacct tctcttgaac ctcttaatat ttcgtatcct actatatctt ttataatatt
      901 tccatctgta tctacaggtg gaagtatatt ttcaaattta acacctaata ctattatata
      961 ttctttacca tcattatctt ttctacttat agcaatagtt tcatcagaag gcattttatg
     1021 atgtcttata ggcttattac ataaatcacc ccatatttct ggattagtgg gatatttttc
     1081 tgtagattcc caataagaca tctcacctga tttaacaata acaccttcga caccttctat
     1141 actatcatta atagtttgtc caattaaatt attagattca gtagatgtgt catatacttg
     1201 ccatctttct gtttctgtag tatatactct attaggatct cctacaacag aatttctatc
     1261 tgtagcatta ggtgctcttc cagggatatg ataagaagca ctccttgctc ctgttttata
     1321 aatccatctt ataaaaaaag aatatacttc atctctcata tatcctacag aagtacctcc
     1381 tgaaaaataa tattctttag gatattctac agcaacccat ttacttttta ttttattagc
     1441 taaaggttga taattaaaat aaggttgaga tgtaacacca catctaacta aatgatgatt
     1501 tatttcaaac attttatcag atttctcata taccatattt ttcattggta tagtacttaa
     1561 atctacagtt ggtaaagatt gaagtatctg atctaaatgt atggtttcaa tattaatagg
     1621 ataatgtcct atttttttag caatagtttg ttgatttaca actgctatta ctacaagttc
     1681 aaattcatta aaattttcat ctgtattatt tacaagtact tctatactac caccaatacc
     1741 ttcatgtgac cataatccaa cagcaggaga aggcattgaa taatctgtca atcttattcc
     1801 attttcagaa taagctataa cagccatata agaaccattt ggtaattgtc ctccgccttt
     1861 tgattttcta atctttaaac aaggttgaga tactaatgga tgtaatctaa tagcatcaca
     1921 atctaaagta gcactgactc cttcatctgt acaaggatca tcatcactat taggattagg
     1981 aatataagca ggtttatcta aatttataac tctatcagga ttgtgaccat cttgccaata
     2041 tactgaccat gtgcaatcat aattttcttt acatactgct gtaataggac ttactactgc
     2101 aaaatctaaa cacatagtat tagcattaga attaaatata gaagtatatg tgcaagtagc
     2161 ttcatcaaaa atacctatat cactgttagg ttcatcaggg atagaaccat gtgtaccttg
     2221 tcttgtagaa aataataccc attgtccgtt agatttatag gcataaccaa taatagtata
     2281 aggagctgaa gcacattctt tattagaagg ttcatttcct attgtaccta cttctccata
     2341 atgtgtatta ttaattgcat ttacagcatg attccaaaga ccttctgaca tatacatgtc
     2401 atttgtatct ttaaacatgc cttttgaaaa agtattagtt tttactatac tggtattttg
     2461 aaaattattt gccataatta tcctttaaac atatcataat atttcatata catagctttt
     2521 ctattcattt gccatgcttt gtgcatttct ttaaaattag gagttctaac aaaacttata
     2581 gcatctattt tagcttttct caattgtata ttcattagat tgaataagtt ttgtaatggc
     2641 tctccattag caataagatt ttcacatatt ct
//
LOCUS       DAOSAB010000076         2663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_25709_length_2663_cov_3.559049, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000076 DAOSAB010000000
VERSION     DAOSAB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25709_length_2663_cov_3.559049"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PK432_01880"
     CDS             complement(<1..520)
                     /locus_tag="PK432_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59707.1"
                     /translation="MDTLHISSMVKKVRLKKPFLISNTFRIYLFIIFTSVYFLLIFPV
                     LQKTINNSMLEYIRGSKEVTLNYLTPLYGQEVYASEYAISTLANSDVTKKYTDNGEIK
                     VEDPRVIAMQKFLFDYNSPMYPYAKTFITEADKYGLDWRLVASISGVESAFGNIIPRG
                     THNAWGWRGKNKN"
     gene            560..>2663
                     /gene="dnaE"
                     /locus_tag="PK432_01885"
     CDS             560..>2663
                     /gene="dnaE"
                     /locus_tag="PK432_01885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOK59708.1"
                     /translation="MFTHLHLHTEYSLLDATIRLPDLIQKLQDSGMKACAVTDHGNMY
                     GAYKFVSAMKEAGLKPILGCEIYIAPRGMDKKDFGIDNKYFHLVLLAKNLTGYKNLVK
                     IVSIAHMEGFYYRPRIDIDTLSKYTEGLIALSACLVGPISVPLLDGKYEKALETAKKY
                     SEIFKNNFFIELQRNGMKEQEIVNEGLLKIAKELDLPIVATCDSHYLNKEDAQIQEIM
                     WCIADGKSWDDSERRMMPTNEFYVKTPKEMKELFKDIPEAIENTQKIADMIEEYDIAF
                     DRIEPKYLDLPKGETSKSYLRKLTLEGMKKKYNEVTDSLKERVEYELKIINDKMYNDY
                     FLVVRDFVMFCRQNGIVVGMRGSGCGSVVAYCTDITDIEPISWELYFERFLNPERDSP
                     PDFDIDIADQRRDELIQYTIQKYGFDNVKQIGTFSKFQTRQAIKDVGRVLGVELSVTD
                     KLAKMVEILFGKSRDIDYMIEHNPEFREIIYSSEKTKRLVDIVKKIAGMCRGVSTHAC
                     GIVLTPQPVTQYCPIQKDAHGEGIGMTQFEMTDLEHIGLMKFDFLGLRNLNIIGEAIT
                     KIKVNKGVDIDLHKIDYNDKEVFDLIQSGHTVGIFQMESEGMKRSIKALKPQSLEDIC
                     YLLAAYRPGPMQYISEYVAVKHGKQKPDYIFPELEPVLSVTYGVITYQEQVMKIAQVI
                     AGYSLGAADLLRRAMGKKK"
BASE COUNT          938 a          360 c          521 g          844 t
ORIGIN      
        1 catttttatt cttccctctc caaccccatg cattgtgtgt tccccttggt attatattcc
       61 caaatgcgga ttcaactcca gaaatagagg caacaagtct ccagtcaagt ccatatttat
      121 ccgcttctgt tataaatgtc ttagcatatg gatacatagg agagttgtaa tcaaacaaaa
      181 acttttgcat tgcaataact cttggatctt caacttttat ttctccatta tcagtatatt
      241 tttttgttac atcagaattt gctaatgtac taattgcata ctcactagca tatacctctt
      301 gtccataaag aggggttaga tagtttaatg ttacctcctt actccccctt atatattcca
      361 gcattgaatt attaatagtc ttttgaagta caggaaagat gagaaggaaa taaaccgatg
      421 tgaatattat aaatagatat attctaaatg tattagatat taaaaatggc tttttgagcc
      481 taactttttt aaccatactt gaaatatgca gagtatccat ataatctagt atatcagaac
      541 tagatgagct gtaaaatata tgtttaccca tttgcatttg catacagagt attctctgct
      601 tgatgctaca attcggttgc ctgatttaat acagaaacta caggatagtg gaatgaaggc
      661 atgtgctgtt actgatcatg gaaatatgta tggagcatat aaatttgttt ctgcaatgaa
      721 agaagcagga ttaaagccta tacttggatg tgaaatatat attgctccta ggggtatgga
      781 taaaaaagat tttggaattg ataataaata ttttcattta gtacttctcg ctaaaaatct
      841 tacgggatat aaaaatcttg taaagatagt ttcaatagcc catatggaag gcttttatta
      901 tagaccaaga attgatattg atacactatc taaatataca gaaggattaa ttgctctttc
      961 tgcatgtttg gtagggccta tttctgtccc tttacttgat ggcaaatatg aaaaggcact
     1021 ggaaactgca aaaaaatatt cagagatttt taagaataat ttctttattg agcttcagag
     1081 aaatggaatg aaagagcagg agattgttaa tgaaggactt ttaaagattg caaaggagtt
     1141 agatttacct attgttgcta catgtgattc tcattactta aataaagaag atgctcagat
     1201 tcaagagatt atgtggtgta ttgcagatgg gaaaagctgg gatgattctg aaaggagaat
     1261 gatgcctacc aatgagtttt atgttaagac tccaaaggaa atgaaagagt tgtttaagga
     1321 tatcccagag gccattgaaa atactcaaaa aattgcagat atgattgaag aatatgatat
     1381 tgcatttgat agaatcgagc ctaaatatct tgatttacct aaaggagaaa catctaaatc
     1441 atatctaaga aaattaactc ttgaaggaat gaagaagaaa tataacgagg tgacagactc
     1501 attaaaagaa agagttgagt atgagttaaa gataattaat gacaaaatgt ataatgatta
     1561 tttccttgtt gttagagatt ttgtaatgtt ttgtagacaa aatggaattg ttgtaggaat
     1621 gagaggatca ggatgtggtt ctgttgttgc atattgtacg gatattactg atattgagcc
     1681 aatatcatgg gagttgtatt ttgagagatt cttaaatcct gaaagagact ctccgcctga
     1741 ttttgatatt gatattgcag atcaaagaag agatgagcta atacaatata caattcaaaa
     1801 gtatggattt gacaatgtta aacagatagg aacatttagt aaatttcaaa caagacaagc
     1861 aattaaggat gtgggaagag tattaggagt tgaactaagt gtgactgaca aattggcaaa
     1921 aatggttgaa attctctttg ggaaatcaag agatattgat tatatgatag agcacaaccc
     1981 tgaatttaga gaaataatat actcttcaga gaagactaaa agattggtag atattgttaa
     2041 aaagatagct ggaatgtgta gaggtgtatc tacacatgca tgtggaattg tacttacacc
     2101 acagcctgtt acgcagtact gccctattca aaaagatgca catggagaag ggataggaat
     2161 gacacaattt gaaatgacag atttagaaca tatcgggtta atgaaatttg attttcttgg
     2221 tttaagaaac cttaatataa taggggaggc aataacaaaa ataaaagtaa ataaaggggt
     2281 tgatattgat cttcataaga ttgattataa cgacaaagag gtgtttgacc tcatccagag
     2341 tggacatact gttggaattt tccagatgga aagtgaaggt atgaaaaggt ccattaaagc
     2401 acttaaacct caaagcttag aggatatttg ctatcttctt gctgcatata gacctggacc
     2461 aatgcagtat atctctgagt atgttgcagt taaacatggt aaacagaaac cagattatat
     2521 ctttccagag cttgaacctg ttctttctgt tacttatgga gtaattacat atcaggaaca
     2581 ggtcatgaaa attgctcaag ttattgcagg ttactctctt ggtgcagcag atttgcttag
     2641 gagagcaatg ggtaaaaaga aaa
//
LOCUS       DAOSAB010000077         2637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_26055_length_2637_cov_3.237026, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000077 DAOSAB010000000
VERSION     DAOSAB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26055_length_2637_cov_3.237026"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PK432_01890"
     CDS             complement(<1..568)
                     /locus_tag="PK432_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOK59709.1"
                     /translation="MQTYDVKMPLEQIRNIGIIAHIDAGKTTTTERLLYFTGQIHKIG
                     EVHEGAATTDFMEQERERGITIQSAAVTCFWDFDGKRYRINIIDTPGHVDFTAEVERS
                     LRVLDGAVVVFDGRKGVEPQSETVWRQANKYHVPRICFLNKINLIGGDFDKSFETIKQ
                     RLSNKAVALTIPIGRESQLHGYVDLLYNK"
     gene            complement(632..2062)
                     /locus_tag="PK432_01895"
     CDS             complement(632..2062)
                     /locus_tag="PK432_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK59710.1"
                     /translation="MKNFKKYFLFICILFLSPFILSGCTVSEEKVREYERIVSDADLL
                     IKGKEYSLALDKLNDATEIIPSRVDAFERMVSIFTVKNKPEDAAKVIEESGGQLSQSD
                     KAKLYTLVGDAYYNLGEFDKAVTNYQLALGMSDNDMSASLGCAKAYLQVGQIEKAKDL
                     LEKTYEGDMVIESTLLLSYIQALSDAQKAEEILKGVEPGDNWREAYTKWSEVLDSLND
                     DELFNAAKLGKEYVDRGYPYLAIALLEPKLSKMGEYADGLYILGKAYYENGDYRKSVD
                     VLQDISTLGDLNQYIYWVLARNYYQLNDINSSIAYYDSAISYGASQAHSAMYKEYLDI
                     LMKENLTEKALEVMRSAEKIFTDTWVPLYYVKIYSLRDDSEKFTYYVNKVSYEDLDDS
                     EKAEFLYSKGSYLIKKSKFDEAQEVLDIFWELNQYDPRYNLLVAEISIKDNDFEKAKE
                     YAKKAIEYDLKGVVSKEAQTLLAQID"
     gene            2133..>2637
                     /locus_tag="PK432_01900"
     CDS             2133..>2637
                     /locus_tag="PK432_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HOK59711.1"
                     /translation="MDSQKSCKNNESNKHVLISLLLIILGVFSFFLVIKSDELVFASQ
                     PLIPIDGKIREALFLPDPLQEKASYGPKLITIQRGGTAFNTLTTLSNVYDILRESSIE
                     LNKNDYVYLNTEYVINGSIIKIVKTTNTIFEMLEEIPFEKEVIENPQLLKGEEKIIQE
                     GKSGIKKQ"
BASE COUNT          870 a          534 c          349 g          884 t
ORIGIN      
        1 ccttgttgta aagcaaatca acatatccat gaagctgact ttctcttcct attggaatag
       61 taagtgctac tgccttattg cttaatctct gttttatagt ttcaaaactc ttatcaaaat
      121 ctcctcctat aaggtttatt ttgtttaaaa agcatattct aggaacatga tatttattag
      181 cctgtctcca tactgtttca gattgtggct caacaccctt cctaccatca aaaacaacaa
      241 ctgcaccgtc aagtaccctt aaagaccttt caacctctgc agtaaaatcc acatgaccag
      301 gtgtatcaat aatattaatt ctatatctct ttccatcaaa atcccaaaaa catgtaacag
      361 cagcagattg aatagtaatt cctctttctc tttcctgctc cataaagtca gtagtggcag
      421 caccttcgtg tacctcccca attttatgaa tctgccctgt aaagtacaaa agtctttcag
      481 tagtagttgt ttttcctgca tctatatgag cgataattcc aatattcctt atctgctcaa
      541 gaggcatttt tacatcatag gtttgcatat tttaaaagta taaattaaag cctaaatgga
      601 aatcttagga cacgagagcg attataccaa actaatctat ctgtgccaac agtgtctgag
      661 cttcttttga tacaactccc tttaggtcat actctatagc tttcttagca tactcttttg
      721 ctttctcaaa atcattatct tttatactaa tttctgcaac aagcaggtta tatctcggat
      781 cgtactgatt aagctcccaa aagatatcta aaacctcttg tgcttcatca aactttgatt
      841 ttttgataag atagctacct tttgaataca aaaactctgc cttttcacta tcatctaaat
      901 cctcatatga tactttgttt acataatatg taaacttttc actatcatct cttaaagaat
      961 atatttttac ataataaagg ggaacccatg tgtcagtaaa aatcttttca gcacttctca
     1021 ttacctctaa ggctttctct gttaaattct ctttcataag gatatctagg tactctttgt
     1081 acattgcaga atgagcctgc gaggcaccat atgaaatagc actatcataa tatgcaattg
     1141 agctatttat atcattaagc tgataataat ttcttgcaag cacccagtaa atatattgat
     1201 taaggtctcc tagagtagaa atatcctgca acacatctac acttttgcga taatcaccat
     1261 tctcataata tgccttccca agaatataca atccgtcagc atactccccc attttgctta
     1321 acttaggctc gagaagagca atagcaagat atggatatcc tctatcaaca tactcttttc
     1381 caagtttagc agcattaaaa agttcatcat catttaaact gtccaacacc tcactccact
     1441 tagtatatgc ctctctccaa ttatcgccag gctctacacc ttttaaaatt tcttctgcct
     1501 tttgtgcatc gcttaaagct tgaatatatg aaagaagaag agttgattct ataaccatat
     1561 ctccctcata tgtcttctca agaaggtctt ttgctttctc tatttgtcca acttgaaggt
     1621 atgctttagc acatccaagt gaagcactca tatcattatc agacattcct aatgctaact
     1681 gataattagt tactgcttta tcaaactctc caagattgta atatgcatca ccaacaagtg
     1741 tataaagttt ggctttatca ctctggctaa gttgtccccc agactcttca ataaccttcg
     1801 ctgcatcctc tggcttattc ttaacagtaa aaatactcac cattctttca aaagcatcta
     1861 ctctggaggg tattatttca gtagcatcat ttagtttgtc aagagctaaa gaatactctt
     1921 ttcctttaat taaaagatct gcatcagata caatcctttc atactcacga actttttcct
     1981 ctgatacagt acaccctgaa aggataaaag gtgataaaaa tagtatacag ataaataaaa
     2041 aatacttttt aaaatttttc atatcttgta ttttacacat cttttgacaa tcatgctata
     2101 ctttgaatat cccgtaatat actgggatag atatggatag tcaaaagtct tgtaaaaaca
     2161 atgaatctaa taaacatgta cttatatctc tcctccttat aattttagga gttttctcct
     2221 tttttctagt tattaaatct gatgaacttg tttttgcaag ccaaccatta ataccaatag
     2281 atgggaagat aagggaagca ctatttttac ctgacccatt acaagagaaa gcctcctatg
     2341 gaccaaaatt aattactatt caaagaggag gtactgcttt taacactctt actacactta
     2401 gcaatgtgta tgacatatta agagagagca gcattgaact taataaaaat gattatgtat
     2461 accttaatac agagtatgta ataaatggaa gtataataaa gattgtaaaa acaacaaaca
     2521 caatatttga aatgcttgag gaaattcctt ttgaaaaaga agttattgaa aatcctcaac
     2581 ttctaaaagg agaagaaaaa ataatacaag aaggcaaaag tggaattaaa aagcaag
//
LOCUS       DAOSAB010000078         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_26378_length_2613_cov_2.569977, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000078 DAOSAB010000000
VERSION     DAOSAB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26378_length_2613_cov_2.569977"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="PK432_01905"
     CDS             complement(<1..1568)
                     /locus_tag="PK432_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59712.1"
                     /translation="MLEKNGSEGFKLEANSTESDKLAPAVVLYIIKSLTKIDKQGKPV
                     LNELGFAEPIEFAPFWRQLMDKASGETSVLGLYNRLKEAEDKGFPLFGQLLKKIFINK
                     VEQDKEGKITRVYTPEETLQSMLTVKNSSIGDLWMKFIQATNLHRIDLVSTTIEVDGD
                     GNLILKSGKTTADYQPILKLWANKFQDQSKSLFVGKTKDNINYIKTEQVVKAFINVDK
                     GKYYVNREDYLPFLNSIGIYLTDVAEVRDGLKADDIDYIAEAIVKHNEYYKDISDLKE
                     SKITSIRDFLTIDHKAEKITNQASRFNYLAELEAMYSEEYASSMKFTPDGNLKSIHAL
                     NSSTTQKVKALNSAQRKDYLFSTNDEYVHMNFLNPEFNPLAKTNILLNSLFSKTEEGQ
                     RIKDNFIEIVDRAGTNDIVKGQYDKGGVYAKMGINDKVISDFIATLDGGYMHSVTPGD
                     KSSYFSIVLDKIFTYEGKKNPKLYVDTEAWLKDDNGNYIRGYNPMVKFFQILYPKLEA
                     EVKRINMIKQGWDK"
     gene            complement(1611..>2613)
                     /locus_tag="PK432_01910"
     CDS             complement(1611..>2613)
                     /locus_tag="PK432_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59713.1"
                     /translation="LLFNVVNSDAWADYENAVINLYEGSDYTHAYHEAWHAFSQVYLT
                     KSERNKLYEAVSKLSGSFKVVRKLPAADGTFTYELQNVKFSDAERIELEEFIAEEFRN
                     YAMNGGKFKVKNEKTSFLSKIFDRIWKALKALVGGSTDVNVYSNPGSSGVLSDIFNTL
                     YTAKTPEQFLPYKASMKNAEFGKLNSGVVASDPNDNLSQSEALLFTKTIDGIFSDIIS
                     NQVYNKGNFNASVGVFDNPKALSEIYKVAKARLDAKRKELVDESKTKEEGSVEKYILD
                     NKISLLTRALKETTYGSIADILNGNSTDTTLIGFHKQNSVFKEMFGKLKISKEEAEE"
BASE COUNT          819 a          494 c          339 g          961 t
ORIGIN      
        1 attttatccc agccttgttt gatcatgttg attcttttta cctcagcctc taatttaggg
       61 taaagaatct ggaagaattt aaccattggg ttatatcctc tgatataatt accattatcg
      121 tctttaagcc aagcttcggt atcaacatat aacttagggt ttttcttacc ctcatatgta
      181 aatatcttat caagtactat tgagaagtat gagctcttat ctccgggtgt tacactatgc
      241 atatatccac catccagggt agcaataaag tcactaataa ctttatcatt tatacccatt
      301 ttagcatata caccaccttt atcatattgt cctttaacta tatcatttgt accagctcta
      361 tctacaatct ctataaaatt atcttttatt ctctgaccct cttctgtttt actaaacaaa
      421 gagtttaata atatattagt tttagcaaga ggattgaact caggatttaa gaagttcatg
      481 tgtacatact catcatttgt tgagaatagg taatcctttc tttgagcact atttaatgct
      541 tttacttttt gtgtagtact actgtttagt gcatgaatag atttaagatt tccatcaggc
      601 gtaaacttca tgctactagc atactcttct gaatacattg cttctaactc ggctagataa
      661 ttaaatctac tggcctggtt agtaattttt tcagctttat gatctattgt taaaaaatct
      721 cttatactag ttattttact ttctttaaga tctgatatat ctttataata ctcattatgt
      781 ttaactatag cttctgctat ataatctata tcatctgctt taagaccatc tcttacttct
      841 gcaacatcag ttaagtaaat accaatagaa tttaagaaag gaagataatc ttccctattt
      901 acatagtatt tgcctttatc aacatttata aaagctttaa ctacttgttc agtttttatg
      961 tagttaatat tatctttagt cttgcctaca aatagagact tagattgatc ctgaaacttg
     1021 ttagcccaaa gtttaagaat aggttgatag tctgcagttg ttttacctga ctttaatatc
     1081 aagttgccat caccatctac ttcaatggtt gtagaaacaa gatctattct atgaagatta
     1141 gtagcctgaa taaacttcat ccacagatct cctatagaag agttcttaac tgtaagcatg
     1201 ctctgaagag tttcttcagg agtatatact ctagttatct taccctcttt atcttgctca
     1261 actttattta taaagatctt cttaagtaac tgcccaaata aaggaaagcc tttatcttca
     1321 gcttctttta atctattata caaacctagc acagaagttt caccagatgc tttatccata
     1381 agctgtctcc agaaaggagc aaactcaatt ggttctgcaa agcctaattc attaagtaca
     1441 ggtttacctt gtttatctat tttagtaaga ctcttaatga tatataaaac aacagccgga
     1501 gcaagcttat cagattcagt agagttagct tctaacttaa atccttctga gccatttttc
     1561 tctagcatct cagtttgttc atcatcgtct ctagatgaag gatcttgttt tcattcttca
     1621 gcttcttctt tagaaatttt aagcttaccg aacatctctt taaagacact gttttgcttg
     1681 tgaaaaccta taagagtagt atctgtacta ttaccattaa gaatatcagc tatactaccg
     1741 taagtagttt ctttcagagc tcttgttaat aaactaatct tgttatctag aatatacttt
     1801 tctacactac cttcttcttt agtttttgat tcatcaacaa gttcttttct tttagcatca
     1861 agtcttgctt tagctacttt ataaatctca cttaaagctt taggattatc aaatacacca
     1921 acagaagcat taaagttacc tttgttgtat acttgattag atataatatc agagaatata
     1981 ccatctattg tctttgtaaa taacaaagct tcagactgac taaggttatc attaggatca
     2041 gatgctacaa ccccagaatt aagtttacca aactcggcat ttttcatgct agctttataa
     2101 ggcaaaaatt gttcaggagt cttagctgta tataaagtat taaatatatc acttaataca
     2161 cctgaagatc caggattaga atatacatta acatcagtag agccccctac taaagcctta
     2221 agagctttcc atattctatc aaagatctta gataaaaaag aagtcttctc attcttaact
     2281 ttaaacttac caccattcat agcataattt ctaaactctt ctgctataaa ttcttctagc
     2341 tcaattcttt ctgcatcaga aaacttaaca ttttgtagct cataagtaaa agtaccatca
     2401 gcagcaggta atttccttac tactttaaag cttcctgata acttagatac agcttcataa
     2461 agcttatttc tttctgattt agtaagataa acctgactaa aagcatgcca agcctcatgg
     2521 taagcatggg tataatcact tccctcatag agattaataa ctgcattctc ataatcagcc
     2581 catgcatctg agtttacaac attaaataat agg
//
LOCUS       DAOSAB010000079         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_26649_length_2595_cov_3.218504, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000079 DAOSAB010000000
VERSION     DAOSAB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26649_length_2595_cov_3.218504"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PK432_01915"
     CDS             <1..443
                     /locus_tag="PK432_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59714.1"
                     /translation="VYFNTYEDNVLVKSELIDEIIIKKPIKEIKEVGTSMYSLNNIEK
                     KGYNCPFWYSVVDSGPYTQEEKDWLKFMMYCESGCNAESNKSNYKGLFQWSPYWWNKQ
                     FDENIFDGYAQIKHTVEKYRAGEATRSNQWPRCNAKYLKATGGN"
     gene            complement(440..2449)
                     /locus_tag="PK432_01920"
     CDS             complement(440..2449)
                     /locus_tag="PK432_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK59715.1"
                     /translation="MNSKDIILGKLNEQQRVAVQTTEGPLLVFAGAGSGKTRVITNRI
                     AYLITEKNVSPKNIMAVTFTKKAAGEMQERVISLLKELNIDTKDVPLIGTFHSIGALF
                     LRQNARDVGLMSNFSICDSTDTENLIKDILIEKNIDVKQIKPESIAYFISDAKNELIS
                     PEQFPQHYGGFIEDIVADIYPLYQEQLRLQNSVDFGDLIYLTVKLLSENKKVLSYYQD
                     IYRYILIDEYQETNTALYMFAKLISQKYQNICVVGDDDQGIYGWRGADIKNIQSFERD
                     FKNVKVVKLEQNYRSTGNVISAAMSVIQQNVFRVNKKLWTEKDSGNPITVYQARDQDD
                     EAQYIVDEINRLRRNEISLVDIAVLYRTNYQSRAIEEAMLKNGIPYKLVGGFRFYDRK
                     EIKDILSYMRFIFNLKDDLSITRILNTPNRKIGPKSIAAIHSISKKCGCSIGEVLVGA
                     YSIKNSLEKHEEMSDGEYKKVESVMDDIMNFSSIVDLFGSLYFESQGIDVLSVIDLIL
                     EKTKYIEWFDDGSQEAEYKKENIEELKNVAAVYSQKFGNTSLEIFLQEINLIELEQSK
                     NQDGSGNYVNLMTLHSSKGLEFDYVFIVGVEEGILPHSRSFTDEEELEEERRLCYVGI
                     TRAKEKLYLTFSERRLSREGYSTQIPSRFLGEIPQDICDYYSWQS"
BASE COUNT          873 a          494 c          324 g          904 t
ORIGIN      
        1 aagtatactt taacacatat gaagacaatg tacttgttaa aagcgaatta attgatgaaa
       61 ttataattaa aaaaccaata aaagagataa aggaggtagg aacatctatg tactctctta
      121 ataatattga gaagaaagga tacaactgtc cattctggta ttcagttgtg gactcaggtc
      181 catatacaca agaagaaaaa gattggctta agtttatgat gtactgtgaa tcaggatgta
      241 atgcagaatc aaataaaagt aattacaaag gcctctttca atggagtcct tactggtgga
      301 ataaacagtt cgatgaaaat atctttgatg gatatgcaca aattaaacat actgtagaaa
      361 aatatagagc tggagaagca accagaagta atcaatggcc aagatgtaat gcaaaatatc
      421 ttaaagctac aggaggaaat taactctgcc aactgtaata atcacaaata tcctgtggta
      481 tctcacctaa gaatcttgac ggtatctgcg tagaatatcc ctctctgctt aatcttcttt
      541 cagaaaaagt taaataaagc ttttcttttg ctcttgtaat accaacataa cacaatcttc
      601 tttcttcttc taactcttct tcatccgtaa atgatctaga atgaggaagt attccctcct
      661 caactcctac tataaagaca taatcaaact ctaacccttt tgatgaatgt agtgtcataa
      721 ggttaacata gtttccactt ccgtcttgat ttttactttg ctcaagttct ataaggttta
      781 tttcctgcaa aaatatctcc aaagaagtat ttccaaactt ctgtgaatac acagctgcaa
      841 cattctttaa ttcttcaatg ttctctttct tgtactctgc ctcctgagaa ccatcatcaa
      901 accattcaat atattttgtt ttctctaata tcaaatctat aacagataga acatctattc
      961 cttgactttc aaaatacaaa ctgccaaata aatcaacaat ggatgaaaag ttcataatat
     1021 catccattac agattcaacc tttttatact ctccatcaga catctcttca tgcttttcta
     1081 aagaattctt tatactatat gcaccaacta aaacctctcc tatactacaa ccacattttt
     1141 ttgaaataga gtgtatagct gcaatcgact taggcccaat ctttctatta ggagtattca
     1201 atattctagt tatacttaaa tcatctttaa gattaaagat aaatctcata tagctaagaa
     1261 tatcctttat ctctttcctg tcataaaatc taaaacctcc aaccagcttg tatgggatac
     1321 catttttaag cattgcctct tcaattgctc ttgactgata gttagttctg taaagaacag
     1381 ctatatcaac taaagatatc tcatttcttc ttaatctgtt aatttcatct acaatatact
     1441 gtgcttcatc atcttgatct cttgcttgat atacagtaat agggttacct gaatcttttt
     1501 ctgtccaaag ctttttattt accctaaaaa cattctgctg aataactgac attgcagcag
     1561 atataacatt acctgttgat ctataattct gttcaagctt aactaccttt acatttttaa
     1621 aatccctttc aaaagactgt atatttttaa tgtctgcacc tctccatcca taaatacctt
     1681 ggtcatcatc tccaactaca catatattct gatacttctg tgaaataagt ttagcaaaca
     1741 tatatagtgc agtatttgtt tcttgatact catcaattaa aatatatctg taaatatcct
     1801 gataatatga aagcactttt ttattttcag ataaaagttt aaccgttaaa tatattaaat
     1861 cgccaaaatc aacagagttc tgtaatctca attgctcctg atacaaagga tatatatctg
     1921 caacaatatc ttctataaaa ccaccataat gctgtggaaa ttgctcaggt gaaatcaact
     1981 cattcttagc atcagatatg aagtaggcaa tagactctgg ttttatttgc ttaacatcaa
     2041 tatttttctc aataaggata tcttttatta aattctctgt atcagtagaa tcacatatag
     2101 aaaagttgga cattaaacca acatctcttg cattttgtct taaaaataat gctcctattg
     2161 agtgaaatgt tcctataagg ggaacatctt tagtatcaat attcaactct ttaagtaaac
     2221 tgataactct ctcctgcatt tctcctgctg cttttttagt aaatgtaaca gccataatat
     2281 ttttaggaga tacattcttc tctgttatta aatatgcaat tctatttgta ataactcttg
     2341 tttttcctga tccagcacca gcaaaaacaa gaaggggacc ttctgttgtc tgtacagcaa
     2401 ctctctgctg ctcatttaat tttcccaata ttatatcttt ggaattcaca gcattgatta
     2461 tatctaagac tgtatacaca tacaattaaa gaaggttata aggtgaaaaa tatattaaat
     2521 ctctttagca tacttctgat atacccaccc tgtttgatct tttccaaact taatctgata
     2581 ccatccctct tcttt
//
LOCUS       DAOSAB010000080         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_26932_length_2576_cov_3.790559, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000080 DAOSAB010000000
VERSION     DAOSAB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26932_length_2576_cov_3.790559"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1403
                     /locus_tag="PK432_01925"
     CDS             228..1403
                     /locus_tag="PK432_01925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK59716.1"
                     /translation="MPKVQNYNIAVVADPLYKKGGAEVHLKYILDTFDNKTLFTAFCD
                     KEFVKREFPNVVVKTSFMQYLPWKFKLKHFYNLFLPLAYRSFRFNGYDAILSLSISFA
                     KFAHGSIPHVNICMSPPKFLWDKEKRSIKSSEQLKGVNKFLFKIYSLFENTFIEDKWR
                     DKDKRAAQRVDRMIAISNAVKERIKKYYDIEADVIYPPVDVTGIEKASRGYRKENWFL
                     YLGRVETYKGVELAIRAAYSAKVPLKIGGIGDDLDRMKNLVRDLNAKGLVKFLGYFSD
                     EEKPVLLAKAKSLIFPVKGEDFGIIPVEANAAGTPVIAYKDGGVVETISDTNPKTGIF
                     FDKYNYKSLAKVLKKFDEKEFKPDNCRKQAEQFASEIFQYKLRTYVEDVLKERQNNG"
     gene            1396..2013
                     /gene="recO"
                     /locus_tag="PK432_01930"
     CDS             1396..2013
                     /gene="recO"
                     /locus_tag="PK432_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOK59717.1"
                     /translation="MAKDTVIVLKSVNYSEADKVLTVLGKYTGKFSLFAKGIRKINSK
                     NRGNMQTLSISDISFYEGRGLPVLTESQLIFSSDIDVVKDRVSDIKRVLDMINKIMQE
                     GDPNVKIFNMLESLCKKNFDVESVNRFRLLFLKEMGYLSDFTVCSVCGSKENLEYLDT
                     SSFALLCKNCYSKRGSGIKIGGDIYKSREFSTALDVYIKKIIEEI"
     gene            2016..>2576
                     /locus_tag="PK432_01935"
     CDS             2016..>2576
                     /locus_tag="PK432_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOK59718.1"
                     /translation="MRFRTAFVKILTFFLLSFSFFFVIGNVNAQRQTHPVTVNGQITA
                     DIYSTLNINPVTVQIYQPSTVTVRILSSSGVGIQGRTVVIVSPTLVITQPTLPTDSSG
                     RATGWVYSTNPGTFTVSALDTTFGFNIEIQNSKTLYVVPVPVPVLLPEPYYTKGTVNT
                     VVWSNTGTQYAYYVEVALDPDFSNVVG"
BASE COUNT          885 a          316 c          523 g          852 t
ORIGIN      
        1 gacatattcc ctgatgatga ttctgatata gatgtaaaga ttgaagcagt agatgattta
       61 gatgatattg ttgctgaact tgataaggaa gatgacgatg attttccaat agatgacttt
      121 ggtgatggag gtcttccaga actttaatat tcattaaagt atatttctct taaaattatt
      181 aaaaataata ggtataatag ggagatttta atatttctta attaaatatg cctaaggtac
      241 aaaattataa tattgcagta gtagcagatc cactttacaa aaagggaggg gcagaagttc
      301 atcttaaata tatacttgat acatttgata acaaaacact tttcacagct ttttgtgata
      361 aagaatttgt taaaagagag tttcctaatg tagtagttaa aacctctttt atgcaatatt
      421 taccatggaa atttaagtta aagcattttt acaatttgtt tttacctttg gcatatagat
      481 cttttagatt taatggatat gatgcaatac tgtcattatc aatatcattt gctaagtttg
      541 ctcatgggag tatacctcat gtaaatatat gcatgtctcc tcctaagttt ttatgggata
      601 aagaaaagag gagcattaaa agtagtgagc agcttaaggg agtgaacaaa ttcttgttta
      661 agatatactc tctttttgaa aataccttta ttgaagataa gtggagagat aaagataaaa
      721 gagctgcaca aagagttgat agaatgattg caatatccaa tgcagttaaa gaaagaatta
      781 agaaatacta tgatatagag gcagatgtaa tatatcctcc tgttgatgta acaggaattg
      841 aaaaggcaag cagggggtat cgtaaagaga actggttttt gtatttagga agagttgaaa
      901 cttacaaggg tgtagagcta gcaattaggg cagcatattc tgctaaagta ccacttaaaa
      961 ttggtggtat aggtgatgat ttagatagaa tgaagaatct tgtaagagat ttaaatgcta
     1021 aggggttagt aaagttttta ggatatttct ctgatgaaga gaagcctgtt ctgcttgcaa
     1081 aagcaaaatc tttaatattt cctgttaaag gagaggattt tggaataata cctgttgaag
     1141 ctaatgctgc aggtacacct gtaatagcat acaaggatgg cggtgtagtt gaaactattt
     1201 ctgatacaaa tcctaagacg gggatatttt ttgataagta taattacaag tcattagcaa
     1261 aagtattaaa gaagtttgat gaaaaagagt ttaagcctga taactgcaga aaacaggcag
     1321 aacagtttgc atctgagata ttccagtaca aattaagaac atatgttgaa gacgtactaa
     1381 aggagagaca aaataatggc taaagatact gttatagtct taaaatctgt aaactattct
     1441 gaagcagata aggtgcttac tgtacttgga aagtatacag gaaagttttc tttatttgct
     1501 aagggtataa ggaagattaa tagcaagaac agaggtaata tgcagacatt aagtatttcg
     1561 gatatttctt tttatgaagg tagagggctt ccagttttaa cagagtctca actaatcttt
     1621 tcctctgata tagatgttgt aaaagataga gtaagtgata ttaaacgggt gttggatatg
     1681 attaataaaa taatgcagga gggtgatcca aatgtaaaaa tttttaatat gcttgagagt
     1741 ctttgtaaaa agaactttga tgttgagagt gttaataggt ttaggctact gttcttaaaa
     1801 gagatgggat atttaagtga ttttactgtt tgtagtgtat gcggaagtaa agagaatcta
     1861 gagtatcttg atactagcag ctttgctctt ctgtgtaaaa actgctacag taaaagagga
     1921 agtggtatta agataggggg tgatatatat aagagcaggg agtttagcac agctttggat
     1981 gtatatataa agaaaattat tgaagaaatt tagatatgag atttagaact gccttcgtaa
     2041 aaattttgac cttttttctg ttatcttttt ctttcttttt cgttatagga aatgttaatg
     2101 cacaaaggca aacacaccct gttactgtta acgggcagat aactgctgat atatattcta
     2161 ctttaaatat taatcctgtt actgtacaga tttatcaacc aagtacagtt acagttagaa
     2221 tactatcttc atcaggagta ggtatacaag gtagaactgt tgttatagtt tctccaactc
     2281 tagttattac tcagccgact ttacctactg attcttctgg tagagcaaca ggctgggtgt
     2341 attcaacaaa tcctggtaca tttactgtaa gtgctctgga tactactttt ggatttaata
     2401 ttgaaattca aaatagtaag acactgtatg ttgtaccagt accagtgcct gtacttcttc
     2461 cagaacccta ctatacaaag ggtactgtaa atactgttgt ttggagtaat actgggactc
     2521 aatatgctta ctatgttgag gtagcacttg atcctgactt tagcaatgta gttggt
//
LOCUS       DAOSAB010000081         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_26998_length_2572_cov_3.413588, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000081 DAOSAB010000000
VERSION     DAOSAB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26998_length_2572_cov_3.413588"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            537..1589
                     /locus_tag="PK432_01940"
     CDS             537..1589
                     /locus_tag="PK432_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOK59719.1"
                     /translation="MDELLLYAIKHNASDLHISVGYPPIVRIDGTLMEVSDKVITAEE
                     AEELIFSVLSDEKKELLEVNREIDLAYFYEGDIPARFRINAYYTKKNLAAAFRIIPSR
                     IRTIDELFLPSIYHQFAQLKQGFILVTGPTGHGKSTSLAAILEEINMTRFAHVITIED
                     PIEYVFEGKKSLIDQREMNDDTHSWPIALRSALRQDPDVILVGEMRDYETIASAITLA
                     ETGHLVFATLHTNSAAQTIDRIIDVFPHNQQQQIRMQLSNILEAVVAQRLIPLDKGGR
                     RVVCEVMIANAAIRNMIREAKTHQIDNVIRTSSDIGMVSLERSLVNLVREGVISVQKA
                     QEYAVYPEEVLRLLKS"
     gene            1594..>2572
                     /locus_tag="PK432_01945"
     CDS             1594..>2572
                     /locus_tag="PK432_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOK59720.1"
                     /translation="MPNFTYSARDNNGKVIEGKLEARDAQAVAEILQDRGLIVVNIKE
                     KKSFDLEELSQINIGGVPMKDKVVFMRQLATMVNAGLPLTRGLQIMEQQITNPLFKKT
                     ISDVKVSVEAGKSLADSFRNTEEVFDDITVNLIEAGESSGNLGEILNKLATELENKNK
                     LQGKIKGALTYPIILLVIIVAVVVLMMLVLVPAMSDIYTEFGGELPAITKIMVSLSNF
                     FINYWWVLLIAILGIVIGIKVWIDTPNGKRSSDKIILKIPIIGGVISKMQIAQFTRIL
                     ALLLSSGLSISRALELTAMSLSNTMFREVILEAKDEVEKGNSLALPIARS"
BASE COUNT          922 a          370 c          534 g          746 t
ORIGIN      
        1 atgttacagc tcaggatata gataaagagg caagagcaaa tgggatgatt acaatgacgc
       61 aagacgggtt tttaaaggca ctagaaggca ttacctccat tgaagaggtc atgagggtgt
      121 caaaagaata aatttaagat ataatgaaaa agatggaaga caatactgcc caaaacaata
      181 caattcagta tcctaatgta cctcaggata attcgcagat tataaatccc aatatttcta
      241 ctacccctac tccaacaaca tctactagtg gtgtacagcc atctgtccaa acacagacag
      301 gagtttctcc tactgcagaa gttacagctg atgctcaaag tattaaccta gctcccagtc
      361 caagtgatga aggtgttttg aaaggtattg taagtaatcc tcttgaagag attattccag
      421 gtgaaaaagt accaaaagca ctaaaaagag agccaaaaga ggttaatctt gctcagccta
      481 tttctgctaa ggaaccagaa ccaccggtaa ttcagaaatt aaatgaacca aaggaaatag
      541 atgagcttct tctttatgct attaagcata atgcatcaga ccttcatatt tctgttggtt
      601 atcctcctat tgtaagaata gatggaacac taatggaagt aagtgataaa gttataacag
      661 cagaagaagc agaagagctt atattctcag tactatctga tgagaaaaaa gaactgttag
      721 aggtaaatag agaaatagat ttagcatatt tttatgaagg agatattcca gcaagattca
      781 gaattaatgc atactataca aaaaagaatt tagctgccgc atttagaatt attccttcaa
      841 ggataagaac gatagatgaa ctttttcttc caagtattta tcatcagttt gctcagttaa
      901 aacagggttt tatactggta acaggaccta caggacatgg taagagtaca tctcttgctg
      961 caatattaga agagataaat atgacaagat ttgctcatgt aataactatt gaagatccaa
     1021 ttgaatatgt ttttgaagga aagaagtctt tgattgatca gagagaaatg aatgatgata
     1081 cacattcgtg gccaattgca cttagaagtg cacttagaca agaccctgat gtcattcttg
     1141 taggtgagat gagagattat gaaacaatcg cttcggcaat aacactggct gaaacaggac
     1201 atttggtgtt tgcaactttg catactaaca gtgcagctca aacaatagat agaataatag
     1261 atgtattccc tcataatcaa caacagcaga taagaatgca gttatcaaac atccttgaag
     1321 cagtagttgc ccaaagatta attcctcttg ataaaggagg aagaagggtt gtgtgtgaag
     1381 ttatgattgc aaatgcagca atcagaaata tgattagaga ggcaaagact catcaaatag
     1441 ataatgtgat aagaacaagc tctgatatag ggatggtatc attggaaaga tctcttgtta
     1501 atcttgttcg tgagggagtt atatctgtac aaaaagctca ggagtatgct gtatatcctg
     1561 aggaagtatt aagattactt aagagttaaa aatatgccta attttacata ttctgccaga
     1621 gacaacaatg gtaaagtaat agaaggtaaa ctggaagcaa gagatgctca ggctgttgct
     1681 gaaattctcc aagacagagg cttgattgta gtaaacataa aagaaaaaaa gagtttcgat
     1741 ttagaagagc tatctcaaat aaatattggt ggagtaccaa tgaaggacaa agttgtcttt
     1801 atgagacagc ttgcaacaat ggtaaatgca ggtcttcctc ttacaagggg attgcagata
     1861 atggaacagc aaattactaa tcctttgttt aagaaaacaa tttcagatgt aaaagtgtct
     1921 gtcgaagcag gaaaaagtct ggctgattct tttagaaata cggaagaggt atttgatgat
     1981 attaccgtta atcttattga agcaggtgag tctagtggta acttaggaga gatattaaat
     2041 aaattggcta cagagttgga gaataaaaat aagcttcaag gaaaaattaa aggagcactt
     2101 acctacccta ttattttact tgttataatt gtagcagttg ttgttttaat gatgcttgtt
     2161 cttgttcctg caatgagtga tatttataca gaatttggag gggagctccc tgctattaca
     2221 aaaataatgg tttctttaag taatttcttt attaattatt ggtgggtact actgattgca
     2281 attttaggta ttgtaatagg gataaaagtt tggattgata ctccgaatgg gaaaagaagt
     2341 agtgataaaa ttatacttaa aattcctatt attggtggag taataagtaa gatgcagatt
     2401 gctcaattta caaggatttt agcattactt ttgagtagtg gtctttcaat aagcagggca
     2461 ctggagctga ctgcaatgtc tcttagcaat actatgttta gagaggttat tttggaagca
     2521 aaggatgaag ttgaaaaagg taattcatta gcacttccga tagcaaggag tg
//
LOCUS       DAOSAB010000082         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_27095_length_2567_cov_2.873408, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000082 DAOSAB010000000
VERSION     DAOSAB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27095_length_2567_cov_2.873408"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="PK432_01950"
     CDS             complement(<1..1818)
                     /locus_tag="PK432_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59721.1"
                     /translation="MICDISQNNLRENLENYVNGLIAANAMKGFDPKVITKIIFNDIK
                     EETDDAVKALGIAYHVPSMVFRSIAQNPKFGDAKFKRLGYDRTALSEFIDNLERAEDK
                     ILAIAGYVNESLDSIDDIEERVNNVPADTAQVAQMISLKAKGVEAYEDKVLVASNPNK
                     TTIQQAVEYGKNSVSPNETKPGYAYYQELLQTLLRLAAGNSDFTTVSMGGNTGFKLRL
                     MFENDLPKDKVLPGRDKPDADGKIWSNFGIPILVLADNNGNILYFDKEGKKTNEANGV
                     PWYNMLPTNDKSRKTAIESFKATYKQANEKDPSEEEIKNFEDNYNKQSNLLDVLRDEL
                     KYNKETPIILTITGGVQGSYTNIDSHPALAKTLSQFKLSNEEKTKGLQQAKEILKDDV
                     FDIVHIKFDNFNDPISLKNPKTIGKDSDPTILDSIVDIMTNDVKVGGESLTSQEKLNY
                     INQFLALKQNLIEADAEKRRLGRLGYTIQEGPNNTIIFTGGGQTLTDKQSIKDFLANN
                     ATIHWHKETNDKGSYFTYKLTPLEKGLAAERSEEKDYFSFIAERFVPRVILNETTKRP
                     MITNGYLTFERENTVKEVEQKVIETTKEVVENKIPTNIEE"
     gene            complement(1821..>2567)
                     /locus_tag="PK432_01955"
     CDS             complement(1821..>2567)
                     /locus_tag="PK432_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59722.1"
                     /translation="NGDDFKTKEKYALQLSLYKNLLENATGIKVDTNMLRIVPLQISV
                     AVNGDINSITPLFKDTKNTNVEFQEVANKYIPITTKSVTNKITKAENKKKQKQDLAQG
                     TVISTLSFVQPTETVTTTEAPTAQQLNENINREAIELAKKNKYEIVYDNLRWKVRRLN
                     KNSVVLYSPQRDEVTIKNEDIPQLLQVASPVKGASTTDNDTVKKNTENVETSPVSFTD
                     VIKMDQAGNVSIAPVDLEKLKRNMLNALCK"
BASE COUNT          764 a          452 c          341 g         1010 t
ORIGIN      
        1 ctcttcaata tttgtaggta ttttattctc tacaacttct tttgtagttt ctattacttt
       61 ttgctctacc tcctttacag tgttctctct ctcaaaagta agatacccat ttgtaatcat
      121 aggtctctta gttgtttcat ttaagataac cctaggtaca aatctttcag ctataaaaga
      181 gaaataatct ttttcttcac ttctctctgc agcaagccct ttttctagag gagtaagttt
      241 atatgtaaag tatgaaccct tatcatttgt ttctttatgc cagtgtatag tagcattatt
      301 agctaagaag tctttaattg attgtttatc agtaagagtt tgtccaccac ctgtaaagat
      361 aatagtatta ttaggaccct cttgaattgt ataacctaat ctacctaatc ttcttttttc
      421 tgcatcagcc tctataagat tttgctttaa agctaaaaac tgatttatat agtttaattt
      481 ttcttgacta gtaagtgact caccaccaac ttttacatca ttggtcatta tatcaacaat
      541 actatctaag atagtaggat cagaatcttt acctatagtc ttaggatttt ttagtgatat
      601 aggatcatta aaattatcga acttaatgtg cacaatatca aaaacatcat ctttaagaat
      661 ttctttagct tgttgtaatc cttttgtttt ctcttcatta gaaagcttaa actgagaaag
      721 agttttagct agtgctggat gagaatcaat attagtatag ctaccttgaa ctccacctgt
      781 tatagttaaa ataataggtg tttctttgtt atattttaat tcgtctctta aaacatctag
      841 taaattagat tgtttattat aattatcttc aaagttctta atctcttctt cagaaggatc
      901 tttctcatta gcttgtttat atgtagcttt aaaactttca atagctgtct ttcttgattt
      961 atcatttgta ggaagcatgt tgtaccaagg aactccatta gcttcattag tcttcttacc
     1021 ttctttatca aagtataata tgttaccatt attatcagca agtactagaa taggaattcc
     1081 aaagttagac catattttgc cgtcagcatc aggcttatct ctaccgggta aaactttatc
     1141 tttaggtaaa tcattctcaa acattagcct taacttaaag cctgtattcc ctcccatact
     1201 aaccgtagta aagtcagaat tacctgctgc taatcttaat aatgtttgta ataattcttg
     1261 ataataagca tagcctggtt ttgtttcatt aggtgatact gaatttttac catattcaac
     1321 tgcttgttga attgttgtct tgttaggatt agaggcaact aatactttat cttcatatgc
     1381 ttcaacacct ttagctttga gagaaatcat ctgcgctact tgagcagtat ctgcaggaac
     1441 attattaact ctctcttcta tatcatctat agaatcaagt gactcattta cataacctgc
     1501 tatagctaat atcttatctt cagctctttc aagattatca ataaattcag ataaagctgt
     1561 tctgtcataa ccgagtcttt taaacttagc atctccaaat tttggatttt gggcaataga
     1621 cctaaatacc atagaaggaa catgataagc tatacccaaa gctttaacag catcatctgt
     1681 ctcttcttta atatcattaa agataatttt ggttataact ttaggatcaa agcctttcat
     1741 tgcattagct gcaataagac cattcacata attttcaaga ttttctctta gattgttttg
     1801 actaatatca cagatcatta ttacttacat aaagcgttta acatatttct ttttagtttc
     1861 tcaagatcta ctggtgctat tgatacatta ccagcttgat ccattttaat aacatctgta
     1921 aatgacacag gagatgtttc aacattctct gtattctttt ttacagtatc attatcagta
     1981 gtactagctc cttttaccgg agatgcaacc tgtaataact gaggaatatc ctcattttta
     2041 atagttacct catctctttg tggagaatac aaaactactg aatttttgtt taatctccta
     2101 actttccatc taagattatc ataaacaatt tcatacttat ttttcttagc cagctcaata
     2161 gcttctctat taatattctc attaagttgt tgagctgtag gtgcttctgt agttgtaact
     2221 gtttctgtag gctgtacaaa acttaaggta gatataactg ttccttgagc aagatcttgt
     2281 ttttgttttt tcttattttc agcctttgtt atcttattag taacactctt tgtagttata
     2341 ggaatatact tattggctac ttcctgaaac tcaacattgg tatttttagt atccttaaat
     2401 aaaggcgtta tactattgat atctccattt acggcaacag atatttgtaa aggtacaatc
     2461 ctaagcatgt tagtatcaac ctttatacct gtagcatttt ctaatagatt tttatataaa
     2521 gataactgta aagcatactt ctctttagtt ttaaagtcat ctccgtt
//
LOCUS       DAOSAB010000083         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_27097_length_2567_cov_2.264729, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000083 DAOSAB010000000
VERSION     DAOSAB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27097_length_2567_cov_2.264729"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..>2567)
                     /locus_tag="PK432_01960"
     CDS             complement(79..>2567)
                     /locus_tag="PK432_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59723.1"
                     /translation="ETANKFLTGKDGIEKIEKYKKQYPEFAKWFDANHIKKETISSTG
                     KKKIIYERLYAWSVPEIEGSQYYIQTPVKIGNTTININRKPNSKYYFTKVKDTYKTIK
                     DDADRQSKIGTVITNRNEYLPLNKEQFIEQGREAEIADSPYINHKYYAAIKDPTYKQL
                     LDTTKEYHLSNQLGLDRDQKLYMDLPRYPISSVLEKSQRGILFTRWTDRLKSITAGIR
                     ATLGGASVEEANIASAQPEDVAEEFVNPEVEKEHQEFTMLEDGVINPVLDQIPIRGIQ
                     DLPIDRTSYDVIAGLNLYMLQTEKQKLFNKISPLAQAVMQTLDDVDGGIAKINGIKSN
                     LSNISNAARNLTTGKDKSVRSAAFKAFFEREFKGRMYSEKHLDWLNKVTSAITGGASM
                     NYFALNLPSAIKNYWGMLWQMNVEAVAGEYFDFGSMAKGKLRSKTAMAEWTTRIWGGT
                     YNTIDTQMIIRFDPIQGKGEEVIGKDPSRTFQKDLASVSWVYSPRKFMEMEGGLQLFY
                     SMMYHHKVKDENGNQTNISYADAWELKEGKMTLKKGIHPDYGITYNEDGTTTLGKEFK
                     KFQNAVHEKFKDLNGAFAKFEQPQAQMFFAYRLFAFMRRYFTAMFMNRFGSERANFAL
                     ETVRTGYYIEALQSLGKIIMSLGLHAGQLAPSERRALFKTLVDVIQIVAISAIASMLF
                     GWDDDDEDRFEKLRAKSGALGEDDFKLDGWLSNHALTLLLKTQAENQSFIPLPGLGLN
                     NYIDFTSSTSIAFGPTITSGAKLLTDIAQHAMPGDNEDLYYKRDTGPYSWQKEGEAKI
                     WNHFFSMIGFSGSQVSPVKGLESFEAQSRQ"
BASE COUNT          785 a          506 c          371 g          905 t
ORIGIN      
        1 aatattctaa accgaatatt aaaagagttc ttgacaagta aattataggg gcatccgcaa
       61 gggtgtccct attttttttt actgacggct ctgagcttca aaagattcta atcccttaac
      121 tggagatact tgactacctg agaaacctat catactaaag aaatgattcc atattttagc
      181 ttctccttcc ttttgccaag agtatggtcc agtgtcacgc ttatagtaca gatcttcatt
      241 atccccaggc atagcatgtt gagcaatatc agttaacaat ttagctcctg aagtaatagt
      301 aggtccaaaa gctatagatg ttgaacttgt aaaatcaata tagttattta atcctaaacc
      361 tggtagagga ataaagcttt gattttcagc ctgagtttta agcaataaag ttaaagcatg
      421 attagataac cagccatcta gtttaaagtc atcttctcct aatgcaccgg atttagctct
      481 taacttttca aatctgtctt catcatcatc atcccagcca aatagcatag aggcaatagc
      541 tgaaatagca acaatttgaa taacatctac aagagtttta aacaatgctc ttctttcact
      601 aggtgctagt tgtcctgcat gcaggcctaa actcattata attttaccca atgattgtaa
      661 agcttctata taatagccgg ttcttactgt ttcaagagca aagttggctc tttctgaacc
      721 aaatctattc ataaacattg ccgtaaagta tcttctcata aaagcaaaga gtctataggc
      781 aaagaacatt tgagcctgag gctgttcaaa cttagcaaaa gcaccattca gatctttgaa
      841 tttctcatga acagcattct gaaacttctt aaactcttta cctaaagtgg tagttccatc
      901 ttcattgtag gtaataccat aatcaggatg tatacctttt ttaagggtca tttttccttc
      961 ttttagttcc caagcatcag cataagatat attagtttga ttaccgttct catcttttac
     1021 tttatgatga tacatcatag aataaaacaa ctgtaaacca ccttccatct ccataaactt
     1081 tctaggacta tatacccaac ttacactagc taaatctttt tgaaatgttc tagaaggatc
     1141 cttacctata acttcttctc ctttaccttg aatagggtca aatcttataa tcatttgagt
     1201 atctatagta ttataagtac ctccccagat tcttgtagtc cactctgcca tagcagtttt
     1261 acttctgagt ttccctttag ccatagaacc aaagtcaaaa tattcaccgg ccacagcttc
     1321 cacattcatc tgccaaagca taccccaata gttcttgatc gcggatggaa gatttaaggc
     1381 aaagtaattc atagatgctc cacccgtaat agcacttgta actttgttaa gccaatctaa
     1441 gtgtttttca gaatacattc tacctttaaa ttctctttca aagaaagctt tgaaagcagc
     1501 agatcttact gatttgtctt ttccagtagt aagatttcta gcagcattag aaatattaga
     1561 taagttactt ttaataccat ttattttagc tataccacca tctacatcat ctaaagtctg
     1621 cataacagcc tgagctaaag gagatatctt attaaacagt ttttgtttct cagtctgtaa
     1681 catatacaag tttaatcctg ctataacatc gtaagatgtt ctatctatag gaagatcttg
     1741 tatacctctt attggtattt gatctagaac aggattaata acaccatctt ccaacatagt
     1801 aaattcttga tgttccttct ctacttcagg attaacaaat tcctcagcta catcttcagg
     1861 ttgagcggaa gcgatattgg cttcttctac agatgcccca cctaaagtag ctcttatacc
     1921 agctgtgatg ctttttaatc tatctgtcca tctagtaaag agtatacctc tctgagattt
     1981 ttcaagtaca gaacttatag gatacctagg gagatccata tataattttt gatctctatc
     2041 tagacccaat tgattactta agtgatattc tttagtagta tctaaaagct gtttataagt
     2101 aggatctttt atggcagcat agtatttgtg attgatataa ggactgtcag ctatttcagc
     2161 ttctctacct tgttcaataa actgttcttt atttaaagga aggtattcat tacggtttgt
     2221 aataacagta cctatcttag attgtctatc agcgtcatcc tttattgttt tgtaagtatc
     2281 ttttacctta gtaaagtagt acttagagtt aggctttcta ttaatattta ttgtagtatt
     2341 acctatctta accggggttt gaatataata ttgactaccc tctatttcag gaacactcca
     2401 ggcataaagt ctttcataaa taatcttctt ctttcctgta gagctaatag tttctttctt
     2461 tatatgatta gcatcaaacc acttagcaaa ctcaggatac tgttttttat acttctctat
     2521 tttctcaata ccatctttac cagtaagaaa cttatttgct gtttctt
//
LOCUS       DAOSAB010000084         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_27105_length_2566_cov_2.787336, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000084 DAOSAB010000000
VERSION     DAOSAB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27105_length_2566_cov_2.787336"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PK432_01965"
     CDS             complement(<1..435)
                     /locus_tag="PK432_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59724.1"
                     /translation="MEYNLDDIKKSLLEDIFSLTAEQLKQKYLSPNGIITLMFKNIKN
                     VEIKKRKEYGIELNRMKKEIEERIINKPTEIQNEEFERKIDPTAPFDINTDNQKRPRI
                     LDLKGSSHPIAQELKKMLDIFTSMGFDILESRQIDDDFHMFES"
     gene            complement(547..885)
                     /gene="rplT"
                     /locus_tag="PK432_01970"
     CDS             complement(547..885)
                     /gene="rplT"
                     /locus_tag="PK432_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOK59725.1"
                     /translation="MRTKTGTVRKRRHNKVLAKTKGYRMTKSKLYKVAHESVMHAGQY
                     SYNDRRKRAGQFRQVWISRLNAACKNNGIQYNTLISGLKKKNIELNRKVLSDIAVNNP
                     EVFSSIIKSI"
     gene            complement(989..1627)
                     /gene="infC"
                     /locus_tag="PK432_01975"
     CDS             complement(989..1627)
                     /gene="infC"
                     /locus_tag="PK432_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOK59726.1"
                     /translation="MDFNNQRIKRDLGTRINEQIRVPKVLLIDSSGKNIGETDTYVAL
                     QMARDAEMDLVEVGANSNPPVCRIMDYSKYIYEQKKKLRKNKAGKAKELKEFRFSVVI
                     DKYDMDTRIRRALEFLEKGHPVKITVTRKGRQPYQQMKEVFSQILTNFSEYSSIESEP
                     KSESNSISITLKGDGKTKNKQNSNKENQAFQPKGKQESKDDVQTESSRSSKN"
     gene            complement(1652..>2566)
                     /locus_tag="PK432_01980"
     CDS             complement(1652..>2566)
                     /locus_tag="PK432_01980"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOK59727.1"
                     /translation="VYKMETRSYRDLPLKLGEFGTVYRYEKTGELHGLQRVRGFTQND
                     AHIYCTQEQLEGVINETLDLLDIFYKDIGFTNYKFRLSLSDREKNPDKYAGDIEKWQF
                     SENTLRKALTDRKVEFEEMPGDAAFYGPKIDIQAVNVYGKEDSISTLQLDFNLPEKFD
                     IKYVDADGQEKQPFVIHRALIGSFERFFAFLIEHFAGDFPLWIAPDQVAIIPISDKHN
                     KYGKEIYELLNQSGIRVTLDDRTKSMQSKIRDAEKLKIPYLVIVGDKEIETETVSIRS
                     RLNKEIGLMKKQEFLDYLLEEIRLKKHK"
BASE COUNT          687 a          477 c          318 g         1084 t
ORIGIN      
        1 actttcaaac atatgaaagt catcatctat ttgtctgctt tctagaatat caaaacccat
       61 acttgtaaat atatcaagca tttttttcaa ttcttgtgcg atggggtgag aagaaccttt
      121 taaatctaat atccttggtc ttttttgatt atcggtatta atatcaaatg gtgcagttgg
      181 atctatcttc ctctcaaatt cttcattttg aatttctgta ggtttgttta ttattctctc
      241 ctcaatttct tttttcattc tattaagttc aattccatac tcttttcttt tcttaatctc
      301 tacattctta atattcttaa acattaaagt aattattcca ttgggagaga gatacttttg
      361 ttttaactgc tctgctgtaa gtgagaaaat atcttcaagt aaagattttt taatatcatc
      421 tagattgtac tccattttaa aaatgtaaga atattatctt aaaagtatac aatattatat
      481 ttcaaagatt aagttaaaac aaaaagactt actaaaaagt ctttaaaata tctatattcg
      541 caggagctat atagatttaa taattgagga gaaaacttca ggattgttta ctgctatatc
      601 agataacacc tttctattta attcaatatt ctttttcttt aatccactaa ttaaggtgtt
      661 gtattgaatt ccattgtttt tacatgcagc attaagtctt gatatccata cctgcctgaa
      721 ttgccctgct ctttttcttc tatcattata tgagtactgt cctgcatgca tcactgactc
      781 atgggcaacc ttatacagct tgcttttagt cattctatac cctttagtct ttgcaagaac
      841 tttgttatgt cttctttttc ttactgttcc tgttttagtt ctcataatct gttactttaa
      901 attaacaacc tttttcttta gattccttgc attagcagaa tatactattc tgtcatcatc
      961 ctgccttctt ttagatctag ctgatcgctt agttcttaga tgaccttgat gactctgttt
     1021 gtacatcatc tttgctttcc tgtttccctt tgggttggaa agcttgattc tctttattgc
     1081 tgttttgttt gttttttgtc ttgccatctc cttttaatgt aattgaaatg ctgtttgatt
     1141 cactctttgg ttcggattcg atactactat attcagaaaa attagtcaat atctggctaa
     1201 atacttcttt catctgttga tatggctgtc ttccttttct agtgactgta attttaacag
     1261 ggtgcccttt ttctagaaat tccaatgctc ttcttattct tgtatccata tcatatttat
     1321 caattactac actaaatcta aactctttta actcctttgc cttacctgct ttattctttc
     1381 ttaatttttt cttctgctca tatatatatt ttgagtaatc cattattcta catacaggtg
     1441 ggttgctgtt agctcctact tctactaaat ccatttctgc atctcttgcc atttggagtg
     1501 caacatatgt atctgtttct ccaatatttt taccactact gtcaattaac aatacttttg
     1561 gtactctaat ttgttcattt attcttgtac ctaaatctct cttaattctt tgattattaa
     1621 aatccattta ttaaagatgt tataaataaa tttatttatg tttttttagt cttatctctt
     1681 caagaagata atctaaaaac tcctgctttt tcattaatcc tatctcttta tttaatctgg
     1741 accttattga tacagtctct gtttcaatct ctttatctcc tactattaca agatacggga
     1801 tttttaattt ctctgcatct cttatttttg actgcatact ttttgttcta tcatctaaag
     1861 ttacccttat tccactctga ttaagaagtt catatatttc tttcccatat ttattatgtt
     1921 tatctgagat aggaataata gctacctgat caggtgcaat ccaaagggga aaatctcctg
     1981 cgaaatgttc tattaagaat gcaaagaatc tctcaaatga gccaatgagt gctctgtgta
     2041 ttacaaatgg ctgtttttct tgtccatctg catctacata tttaatatcg aatttttctg
     2101 gaagattaaa atcaagctga agagtagata ttgaatcttc tttaccatac acattaactg
     2161 cttgtatatc tattttaggt ccgtagaatg ctgcatctcc aggcatttct tcaaactcta
     2221 cctttctgtc tgttaatgct tttcttaaag tgttttctga aaactgccat ttctcaatat
     2281 ctcctgcata tttatcagga tttttttctc tatcagataa agaaagtcta aacttgtaat
     2341 ttgtaaatcc aatatctttg tagaatatat caagaagatc aagtgtctcg tttattactc
     2401 cttccaactg ctcctgtgta cagtatatat gagcatcatt ttgggtgaat cctcttactc
     2461 tctgtaatcc atgcagttct ccagtcttct catatctgta tactgtaccg aattctccca
     2521 gttttaaagg taaatctctg tagcttctcg tttccatttt gtatac
//
LOCUS       DAOSAB010000085         2536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_27515_length_2536_cov_7.502217, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000085 DAOSAB010000000
VERSION     DAOSAB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27515_length_2536_cov_7.502217"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PK432_01985"
     CDS             <1..1271
                     /locus_tag="PK432_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59728.1"
                     /translation="DLTGRVDDLEGFVIDLDSRLADVEAALVDKPSLDEVEAMIEEKL
                     GSIEVPDVEELTGRVTDLEDRVSAVETRLDDVDSAISDIMSSVDDLNANLVDLSGKVE
                     EVLALKDQLDELIARIESIQGIEIPVELDEIKSFIMDLDSRLTELSTSVDDLSTVVAD
                     LQSQIDDINSKFDQVNTNIDNINSSIEALSTSVDDLFVLNDGVSTRLDETVNRLVTLE
                     ERVATLENKATVEKNFALDLRFTVKVGLATRTPVNTLTTKLTFNLMPNVKLFLQSVSS
                     ASGIDMSGGVDASMPISKDITGVAGASYDNGKFSVYGGVKLAMLKNAVIDVIVGVPDV
                     MALGSGELKDTVSLKIGGSYKLTDKVSLTGYATNLEGGAAAANFAVVYAVDPKADLSL
                     GLYLYGLGNLLGGNFDAVQKSVVFSAGASF"
     gene            complement(1341..2531)
                     /locus_tag="PK432_01990"
     CDS             complement(1341..2531)
                     /locus_tag="PK432_01990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOK59729.1"
                     /translation="MIWDLTIIGGGPVGSFAGYLAGKNNIKTLIIEEHSKIGEPLHCL
                     GKLSIHAFEEFPLPKESIRNSLKGGYFFFPNGNFIKLKKSKPDSYILDRSLFDKNMAE
                     LAGKNGCTLLLSAKAIGIEIENEATRLIIEEKNRRRKIEAKVIINAEGAKRQFAKRLG
                     LKPNPYLVSLQYEVIGLELLDKECVEVYLGSTYSKGFFLWVSPYGNLTKIGVAVQPNE
                     NPKIFLDKFLNNLKKTRNSGIEIIKSYGGIIPILGPYEEYYYPNILIVGDAAGYNKST
                     TGGGIYFGLQGAQIAIEQVKKYLEDNNINHLKTFPKLTYKKFGKELKFTKIVRKFLNQ
                     LSDSDLNEIYKIINSEEIIRNIENFGDTAYQTSVLKTIPNLLKNKEFYKLSKFTPKIL
                     KSIF"
BASE COUNT          782 a          368 c          488 g          898 t
ORIGIN      
        1 gagatcttac agggagagta gatgatcttg agggattcgt tatagatctt gattcaagac
       61 ttgcagatgt tgaggcagca ttagtagata agccatcttt agatgaagta gaggcaatga
      121 ttgaggagaa attaggttca attgaggttc cagatgtgga ggagttaact ggcagagtta
      181 ctgatcttga ggatagggtt tcggcagttg aaacaagact tgatgatgta gatagtgcaa
      241 tttctgatat tatgagttca gtagatgatt taaatgcaaa tcttgtggat ctttctggaa
      301 aggttgagga ggtacttgca ttaaaagatc aacttgatga gttaatagca aggatagaat
      361 ctatacaggg tatagagatt cctgtagaac ttgatgagat taaatcattc ataatggatt
      421 tagactcaag attaacagaa ctttctacca gtgtagatga tctaagtaca gtagtagctg
      481 atttacaatc tcagattgat gatattaatt ctaaatttga tcaagttaat acaaatattg
      541 ataatatcaa ctcttctata gaggctctat ctaccagtgt ggacgatcta tttgtgctta
      601 atgatggagt atctacaaga ttagatgaaa cagtaaatag attagttact ttagaagaga
      661 gggtggctac tcttgagaat aaagctactg tagagaagaa ttttgcattg gatttaagat
      721 ttacagtaaa agttggtttg gccacaagaa ccccagtgaa tactcttaca acaaagttaa
      781 catttaattt gatgcctaat gtaaaactct tcttacaatc tgtgagtagt gctagtggaa
      841 ttgatatgtc tggtggtgta gatgcaagca tgccaattag caaagatata acaggtgttg
      901 caggagcttc atatgataat ggtaagttct cagtttatgg tggtgtgaag cttgcgatgt
      961 taaagaatgc tgtaattgat gttatagtag gagttccaga tgttatggct cttggcagtg
     1021 gagaactaaa agatacagta tctttgaaga ttggtggatc ttataaatta actgataaag
     1081 ttagcttaac aggatatgca acaaatcttg aaggtggtgc agcagcagct aattttgcag
     1141 tagtgtatgc tgtagatcct aaggctgatc tatctttagg attgtacttg tacggtctag
     1201 gaaatctact tggaggtaat tttgacgctg tccaaaaatc cgtagtattc tctgcaggag
     1261 cttccttcta atatctgctg attggaaaat caaggggagg gaagaaaatc cctccccttt
     1321 tttatttcct acaggagtat ttaaaaaatt gattttagaa ttttaggggt gaattttgat
     1381 aacttataaa attctttatt ttttaataaa ttaggaattg ttttaagtac tgatgtctga
     1441 taagcagtat ctccaaagtt ttcaatgttt cttataattt cttcagaatt aattatttta
     1501 tatatctcat tcaaatcact atcacttaat tggtttaaaa actttcttac tatttttgtg
     1561 aattttaact ctttaccaaa ttttttataa gttaacttgg gaaatgtttt taaatgatta
     1621 atattgttat cctcaagata ttttttaact tgttctatag ctatttgggc tccttgtaat
     1681 ccaaagtata tacctccacc tgtggtagat ttgttgtatc ctgcagcatc tccaacaatg
     1741 aggatattgg gataataata ttcttcataa ggtcctaaaa taggtataat tccaccatag
     1801 gactttatta tttctatacc cgaatttcgg gtttttttta gattgtttaa aaatttatca
     1861 agaaatattt taggattctc attaggttgg actgctacac cgattttcgt aaggtttcca
     1921 taaggagaaa cccaaaggaa aaagcctttt gaataagtag aaccaagata aacctctacg
     1981 cattctttat ctagaagttc taatcctatt acttcatatt gaagggatac gagataagga
     2041 ttaggcttga gacctaatct tttggcaaat tgccgttttg caccttcagc gtttattatt
     2101 acttttgctt caatctttct tcttcgattt ttttcttcta tgattagtct tgttgcttcg
     2161 ttttcaattt caattccaat agcctttgct gataacagta aagtacagcc attttttcca
     2221 gctaattctg ccatgttttt gtcaaagaga gaacgatcaa gaatgtagga gtcaggctta
     2281 ctttttttta attttataaa attcccattg ggaaagaaaa aatatccccc ttttaagcta
     2341 ttcctaatgg attccttagg aagagggaat tcttcaaagg catgaatgct tagtttaccc
     2401 aaacagtgta ggggttctcc tattttgctg tgctcttcta ttatgagagt ttttatatta
     2461 tttttccctg ctaagtaacc tgcaaaactt cctacaggac ctccgcctat tatagtaaga
     2521 tcccatatca tattaa
//
LOCUS       DAOSAB010000086         2510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_27890_length_2510_cov_3.946232, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000086 DAOSAB010000000
VERSION     DAOSAB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27890_length_2510_cov_3.946232"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..247)
                     /locus_tag="PK432_01995"
     CDS             complement(83..247)
                     /locus_tag="PK432_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOK59730.1"
                     /translation="MQKYKCTVCGYIYDPQKGDPESGIKPGTPFEDIPDSWYCPLCGA
                     TKKEFVPLEE"
     gene            complement(271..2418)
                     /locus_tag="PK432_02000"
     CDS             complement(271..2418)
                     /locus_tag="PK432_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK59731.1"
                     /translation="MAQKKVIEVKGINKSFSLGKRENKVLKDVSLDIYSGQLICIFGP
                     SGCGKSTLLNVIMGLERPDSGSVRIVGENIWDMDISEQTDFRKKHIGVVYQQQIWIRS
                     LSVIENVALPAQLLGMNKGDALNRAEKVLQDVGMLRFRDFYTSELSSGEQQKIGLARA
                     LITDPEIVIADEPTGNLDTNNGYEVLKVLKELAQKGVTVVLVTHNPEYLDYCDTVVVM
                     KDGSIMNVLKGEKGILSKVQESLNVIKDIKVESRTEYKVSSENSVDTEYYPEESFFEK
                     LGGYIKLIVNFFTKSSSLLVLSLVRKINLNKSTQLRRSMTKSNNGDISLLDLTELSFK
                     NVFFKRFRTLITILGVSLGVGFVVLLVSIGYGVEDLVIDEVTKAQNLNQVDVFPKVGS
                     QLKLNDELVEKVSAISGVSKIYKIKNFPGRIDYKGSTVDVVVYGVESGYLENSPVKKV
                     SGEFLNDESDKFSAIINSEYLKTLGLSTDIIGETLNLNVIQSVPESESEPKNIPVTIV
                     GVVEDDSPPVIYLKIGSVRDYTEDEYSALSIIVNKESSLDFVRKQIESLGLETFSIMD
                     TVNQVQQIFTYVRYGLLAFGILAFAIAFLGMINTLTVSLLERTREVGLMKIIGMKKND
                     IQFLFITESMFIGLLGGIFGILFGILGGYLVSLAVYLLSSSRGVEFMVITSFPFVTAL
                     IVILVTTLLSFFTGLYPANRAVKIPTMDAVRYE"
BASE COUNT          861 a          497 c          303 g          849 t
ORIGIN      
        1 catagaggtt atatccttta ctgtatcttt aataggattc ataggtttaa ataaaataaa
       61 ttaaatataa gtatattata tattattcct ctaatggtac aaactctttc ttagttgcac
      121 cacataaggg acaataccaa ctatcaggta tatcttcaaa aggagtacct ggctttatac
      181 cagattcagg atctcccttt tgtggatcat atatataccc acatacagta catttatatt
      241 tttgcatata aatataaaat ataaaattaa ttactcatat cttacagcat ccatagtagg
      301 aattttaaca gctctatttg caggatacaa cccagtaaag aatgatagga gagttgttac
      361 taatatcaca atcagtgcag taacaaatgg gaaagaagta attaccataa actcaactcc
      421 acgagatgaa gacaaaagat acacagcaag tgatactaga tatcctccaa gtattccaaa
      481 cagtattcca aaaattcctc ctaacagacc aataaacatg gattcagtaa tgaaaagaaa
      541 ctgtatatca ttcttcttca tccctataat tttcattaaa ccaacctctc ttgtcctttc
      601 cagtaatgat actgttaatg tattaatcat tccaagaaaa gctattgcaa atgccagaat
      661 accaaatgcc aaaagaccat atctaacata agtgaatatc tgctgaactt gattaacagt
      721 atccattatc gaaaatgttt caagtcctag agattctatt tgctttctaa caaaatcaag
      781 agatgattct ttattaacaa taattgaaag tgcagaatat tcatcttctg tataatccct
      841 cacagaacct attttaagat atataacagg aggagaatca tcttctacaa ctcccacaat
      901 tgttacagga atgttcttag gctctgattc actttcaggt acagattgta taacatttaa
      961 atttagggtc tctccaataa tgtctgtact tagtccaagt gtctttaaat attcagaatt
     1021 aattattgca gagaatttat ccgactcatc atttaaaaac tctccactaa cctttttaac
     1081 aggagagttc tcaagatatc cactttctac tccatataca acaacatcca cagtggatcc
     1141 tttgtaatca attcttccag gaaaattttt aattttatat atcttgctaa cacctgatat
     1201 tgcagaaact ttttcaacta attcgtcatt taattttaac tgactgccta ctttaggaaa
     1261 aacatcaact tgattaaggt tttgagcttt agtgacctca tcaattacta aatcctcaac
     1321 accataccct attgaaacaa gtagtacaac aaaaccaact cctaagctaa ctcctaatat
     1381 tgtaattaaa gttctaaacc ttttaaaaaa gacattctta aaagagagct ctgttaaatc
     1441 aagcaatgag atatctccat tgttagattt tgtcatactc cttcttaact gtgtagattt
     1501 gttaagatta atttttctta caagagataa gacaagtaat gatgaggact tagtaaagaa
     1561 gttgactatt aatttaatat atccccccaa cttttcaaag aatgattcct ctggataata
     1621 ctcagtgtct acactatttt cactactaac cttatattca gttctacttt ccacttttat
     1681 atcctttatt acatttagac tttcctgtac cttacttaaa attccctttt cccctttcag
     1741 cacattcatt atagaaccat ctttcattac cacaacagta tcacagtaat ctaaatattc
     1801 aggattgtgt gtaaccaaaa caactgttac tcccttctgt gctaattcct ttaatacttt
     1861 taatacttca taaccattat tagtatcaag gttccccgta ggctcatcag caataactat
     1921 ctcagggtca gtaattaaag ccctagccaa accaattttt tgctgttcgc cagatgaaag
     1981 ttcggaagtg taaaaatctc taaatcttag catacccaca tcctgcaaga ctttctcagc
     2041 acgatttaat gcatcacctt tgttcattcc caaaagttgt gcaggaagag caacattctc
     2101 aattacactt aaagatctaa tccaaatctg ttgctgatat accactccaa tgtgtttctt
     2161 tctaaaatct gtttgctcac taatatccat atcccatata ttctcaccca caattctaac
     2221 actcccacta tcaggtcgtt caagtcccat tattacattt aatagtgtag atttcccgca
     2281 tccagaagga ccaaagatac atattaactg gccagagtat atatcgagag atacatcttt
     2341 taatactttg ttttctcttt ttcctaatga aaagctttta tttattcctt taacttcaat
     2401 tacttttttt tgagccatta gaatatgtta aatatattag agatagagtt gaatattgtt
     2461 tctaaaacac tattagatga tttttgagta attgaaacaa gaggaccata
//
LOCUS       DAOSAB010000087         2494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_28130_length_2494_cov_3.394014, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000087 DAOSAB010000000
VERSION     DAOSAB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28130_length_2494_cov_3.394014"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2494
                     /locus_tag="PK432_02005"
     CDS             <1..>2494
                     /locus_tag="PK432_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59732.1"
                     /translation="RIVPNVKTEVTPAAQPAINNTPLHTEITKAEEKIKENKKEESKD
                     IIKPLDDLEIDDINDENIPYELASRSSHPADKMQQELELKWLKENLNADVKEFEGLTI
                     NNNIVLGYVKNQIIFLNSQMRAKGTLYHEAFHYTFRYLFDKPTRDRLITAIQSDRKYD
                     SYFTKAAVSKFRRDRNYNPTISDEEVRRNIAEEILADKFKRFALAKQKENERIGIIRA
                     FLKLLDKIVKLFTRKSNEIDLLFNRIYTGEFKNTQRKQFSDNLTEAYALIPTVRQIVI
                     NPATGRPRTEPYYLSDNEQSQLINMIVAYIAKDKSTKSFDDKFDRACKQLLEEVWSLD
                     TIYALNNATGENDRKKIKDAIGNTVTAYRFLLGDRLNENPNTYDINFTGDDTYSNRIT
                     KNYSIKVDDRLVPNDKGEYSRELLKTMVQKEYEKIVTLEGFIQLIDDIENDVQIEDIE
                     DAVGTGDDKRFDADISEFNVAKQNVGLIRKFLSSIIYIRTNPITGIKTLATADPVETF
                     NTIVQATTNLSDDNIIPSLKHYSEIIREDGYINEANVIEAVYNALVTKEHKEKDSQFV
                     NLFKTVFQTIELPYMFFSVDKTEERGFSYTLKDKLLETDLVAKKKSLYQEFAATFEKE
                     HKSAEYKEHVKNLKNLINKYFESKNLSVKTMVATESIDKVAQSLADAFTGIGFKMTKS
                     SMKLALLQHLSKEYTMEYNLYVPDADKNYLESFSDFIKEQQYFDKTSISSFYNTFLRS
                     DVLLTASSIVNIVEPTNRKVPESLKAFDAQLKKALIFVFKHDPKMKPSVSKNAEGKPI
                     YRYVRSNPLSLIANKFRREGLKATLETDELWDR"
BASE COUNT         1050 a          278 c          368 g          798 t
ORIGIN      
        1 agaattgttc ctaatgtaaa aacagaagta actcctgcag ctcaaccagc aattaataat
       61 acaccattac atactgaaat aacaaaagca gaagagaaga taaaagaaaa taaaaaagaa
      121 gaatcaaaag atattattaa acctttagat gatttagaaa ttgatgatat taatgatgaa
      181 aatattcctt atgaattagc ttcaagaagt tctcatcctg ctgataaaat gcaacaagaa
      241 ttagaactaa aatggttaaa agaaaatcta aatgccgatg ttaaagaatt tgagggattg
      301 actataaata ataatatagt attaggatat gtaaaaaatc aaattatatt tttaaattct
      361 caaatgcgag caaaaggtac tttatatcat gaagcttttc actatacatt tagatattta
      421 tttgataaac caacaagaga tagattaata acagcaattc agtctgatag aaagtatgat
      481 tcttatttta ctaaagctgc tgtatctaaa tttagaagag atagaaatta taatcctact
      541 ataagtgatg aagaggttag aagaaatata gctgaagaaa ttctagctga taaatttaaa
      601 agatttgctt tagcaaaaca aaaggaaaat gaaaggatag gtattataag agccttttta
      661 aaattattag ataaaattgt aaaattattt acaagaaaat caaatgaaat agacttattg
      721 tttaatagaa tttacacagg agaatttaaa aatacacaaa gaaaacaatt ttctgacaat
      781 cttacagaag catatgctct tattcctaca gtaagacaaa ttgtaattaa tcctgctact
      841 ggtagaccta gaactgagcc ttattattta tctgataatg aacaatcaca acttataaat
      901 atgattgttg cttatatagc taaagataaa agtactaaat cttttgatga taaatttgat
      961 agagcttgta aacaattatt agaagaagta tggtcattag atacaatata tgctttaaat
     1021 aatgctactg gagaaaatga cagaaagaaa ataaaagatg ctattgggaa tacagttact
     1081 gcttatagat ttttattagg tgatagatta aatgaaaatc ctaatactta tgatattaac
     1141 tttacaggtg atgacacata ttcaaataga ataactaaaa actattctat taaagttgat
     1201 gatagattag ttcccaatga taaaggtgaa tattcaagag agctattaaa aacaatggtt
     1261 cagaaagaat atgaaaaaat tgttacatta gaagggttta ttcaattaat agatgatata
     1321 gaaaatgatg tacaaattga ggatatagaa gatgcagtag gtacaggtga tgataaaaga
     1381 tttgatgctg atatatctga atttaatgtt gcaaagcaaa atgtagggtt aattagaaag
     1441 ttcttatcta gtattatata tataagaaca aatcctataa caggtattaa aacacttgct
     1501 acagcagatc ctgtagaaac ttttaataca attgtacaag ctactactaa tttatcagat
     1561 gacaatatta taccttcttt aaaacattat tctgaaataa taagagaaga tggttatatt
     1621 aatgaagcaa atgttataga agcagtatat aatgcattag ttactaaaga acataaagaa
     1681 aaagattcac agtttgttaa tttatttaaa actgtttttc aaactataga attaccttat
     1741 atgtttttct ctgttgataa gactgaagaa agaggattct cttatacttt aaaagataaa
     1801 ttattagaga cagatttagt tgctaaaaag aaatcattat atcaagaatt cgcagccact
     1861 tttgaaaaag aacataagtc agcagaatat aaagaacatg taaaaaatct taaaaatctt
     1921 attaataaat attttgaaag taagaatctt agtgttaaga ctatggttgc tacagaatct
     1981 attgataaag tagcacaatc attagcagat gcttttacag gtattggatt taagatgact
     2041 aaatcttcta tgaagctagc tttactacag catttatcca aagaatatac aatggaatat
     2101 aatctgtatg tacctgatgc agataaaaat tatttagaat ctttttctga ttttatcaaa
     2161 gaacaacaat attttgataa aacttctata tcttcatttt ataatacttt tttacgatca
     2221 gatgttttgt taacagcatc tagtattgta aatattgtag aacctactaa taggaaagtt
     2281 ccagaatctt taaaagcatt tgatgctcaa ttaaaaaaag cattaatctt tgtatttaaa
     2341 catgatccta aaatgaaacc atctgtatct aaaaatgcag aaggtaaacc tatttataga
     2401 tatgtaagat caaatccttt aagtttaata gctaataaat tcagaagaga aggattaaaa
     2461 gctacattag aaacagacga attatgggat aggt
//
LOCUS       DAOSAB010000088         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_28348_length_2478_cov_3.324804, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000088 DAOSAB010000000
VERSION     DAOSAB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28348_length_2478_cov_3.324804"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PK432_02010"
     CDS             complement(<1..713)
                     /locus_tag="PK432_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59733.1"
                     /translation="MATYIQGLTDYIPQIQPFQPDLNFYGNVMQAKQTKYDAAKKQIS
                     KLYGSLLYSPMTHDDNIKRRDEFFKVIDNDIKRISGMDLSLQQNVDQALNVFQGFYDD
                     KYMINDMMWTKNYNNAVQKHQALKNCTDPKKCGGIAWDEGLMDLQYKREDFRKMSLDE
                     TLNASAPTYTPYYDWKKDALKFAHDNGYKVTKDVLDGDYIVTRPDGTKIQGLWGLFKE
                     GFGDDPRVQANYETMASVK"
     gene            complement(775..>2478)
                     /locus_tag="PK432_02015"
     CDS             complement(775..>2478)
                     /locus_tag="PK432_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59734.1"
                     /translation="GKFVYDMNLGKYIPMNTAAISPADTAGISQFINPADTVGISQFI
                     NAPRREYGGDLNKYKFGDEFKGNHGKFTQQKNFRKYEDGTVTFIKNGKELIHTDGDPN
                     WKPNIEIKKNKPAGTQGTRMSVGARRVNDAQGYVTNPGEAKKRSQDLTESATADAEGN
                     IPIIIRPYYGSDNPTAYTPEQWQEFARSTGFKPKTKSVGAQNREFQDYLAAHPDWKNT
                     IDELHQKHGRPKEGKFDAMIGRRWDVLMQKKPAPEKLPEETPAPKITIAPEERKNTTV
                     PPQVIKRNSPVPVKGGTYAPWWLQDIVKTSGAAADLARVNRYLPWQATPEVRLPDATF
                     YDPTRELAANAEQANIQTQGLAAFTGPQALSARSSSIQGQAAKNAADIMARYNNMNVE
                     LANQLNQERTSIMNTASQNKAALDTQLFDKYTIVNQQFDNAKNMARQNLRQSYIDAIT
                     NRAKTQALNTLYPNYYTDPSRGGMLGFAPDFSKIPAEQPNDSDEWWDAVGKTGSKELA
                     IEYLKLKNKGAISDPYGERNAYRSSMNYPEEYLKLKNKGAISDPYDERRGYMGAQGYG
                     E"
BASE COUNT          700 a          480 c          387 g          911 t
ORIGIN      
        1 cttttaacag aagccattgt ttcataatta gcttgtactc taggatcatc accaaagcct
       61 tctttaaaaa gaccccataa accttgtatt tttgttccat caggtctagt aacaatatag
      121 tcaccatcta gaacgtcttt agttacttta tatccattat catgagcaaa ctttaaagca
      181 tccttcttcc aatcataata aggagtataa gtaggagctg atgcattaag agtttcatca
      241 agagacattt ttctaaagtc ttctctttta tattgaagat ccataagacc ttcatcccaa
      301 gcgatgcctc cacatttttt aggatcagta cagttcttta aagcttgatg tttttgtaca
      361 gcattattat agttcttagt ccacatcata tcattaatca tgtacttatc atcataaaag
      421 ccttgaaaca catttaaggc ttgatctaca ttttgctgta gagataaatc catgcctgat
      481 attcttttaa tatcattatc aataacttta aaaaactcgt ctcttctttt gatattatca
      541 tcatgagtca taggagaata caaaagactt ccatacagtt tacttatttg ttttttagca
      601 gcatcatact tggtttgctt tgcctgcatg acgttgccat aaaagttaag atctggttgg
      661 aaaggttgta tttgcggaat gtaatctgtt agaccttgaa tgtatgttgc catgattgat
      721 ttttatactg taaatctatt aaatttatat tgaataataa tacacttata aagtttattc
      781 tccatatcct tgtgccccca tataccctct tctttcatca taaggatcac ttattgctcc
      841 tttatttttt aacttaagat attcttctgg gtaattcatg ctgcttctat aagcatttct
      901 ttctccatat ggatcactta ttgctccttt attttttaac ttaagatatt ctatagccag
      961 ttctttgcta ccggtttttc ctactgcatc ccaccattca tctgaatcat taggttgttc
     1021 agctggtatt ttactaaagt ctggagcaaa acctaacata ccacctctag aagggtcagt
     1081 ataatagtta ggatacaaag tatttaaagc ctgagtttta gcacggttag taatagcatc
     1141 aatataagat tgtctcaagt tctgacgggc catattcttg gcattatcaa actgctggtt
     1201 aactatagtg tacttatcaa ataactgagt atctaaagct gctttgtttt gagaagctgt
     1261 attcatgata ctagttcttt cttgattaag ttgattagct aactcaacat tcatattatt
     1321 atacctagcc ataatatccg cagcattctt agctgcttga ccttggatag aagatgatct
     1381 tgctgataaa gcttgtggtc cagtaaaggc tgctaagcct tgtgtctgga tattagcttg
     1441 ttcagcatta gcggctaact cgcgagtagg atcatagaaa gtagcatcag gaagtcttac
     1501 ttcaggagta gcttgccacg gaagatatct gttaacacga gctaagtcag cagctgcacc
     1561 agatgttttt acaatatctt gtaaccacca aggagcatat gtaccacctt taacaggtac
     1621 tggagaattt cttttaataa cttgtggagg aacagtagta tttttccttt cttcaggagc
     1681 aatagttatt ttaggggcag gagtttcttc aggaagtttt tctggtgcag gcttcttctg
     1741 cattaataca tcccatcttc taccaatcat ggcatcaaat tttccctcct ttggtctacc
     1801 atgtttttga tgaagttcat caatagtatt cttccaatca ggatgagctg ctaaataatc
     1861 ttgaaactct ctattttgag caccaacact ttttgtttta ggcttaaagc cggtacttct
     1921 agcaaactct tgccattgtt ctggagtata agcagttgga ttatctgatc cataatatgg
     1981 tctaataata ataggtatat taccttcagc atcagccgta gctgattcag tcagatcttg
     2041 agatcttttt tttgcttcac caggattagt tacataacct tgtgcatcat ttactctacg
     2101 ggctcctaca ctcattctgg ttccttgagt acctgctggt ttattttttt taatttctat
     2161 attaggcttc cagttaggat ctccatctgt atgaattaac tcttttccat tctttataaa
     2221 agttacagta ccatcctcat actttctaaa gtttttttgt tgagtaaact taccatgatt
     2281 acctttaaat tcatctccaa acttgtattt attaagatct cctccgtact ctcttcttgg
     2341 agcattaatg aactgactta ttcctacagt atctgcagga tttataaact gacttattcc
     2401 agccgtgtct gcaggagata tagctgcagt attcatagga atatattttc caagattcat
     2461 atcataaaca aacttacc
//
LOCUS       DAOSAB010000089         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_28810_length_2450_cov_2.951148, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000089 DAOSAB010000000
VERSION     DAOSAB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28810_length_2450_cov_2.951148"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PK432_02020"
     CDS             complement(<1..452)
                     /locus_tag="PK432_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOK59735.1"
                     /translation="MTKPTKWKTQREAFIESLSYLQGRQTGKITSIKTPWQKFNDATT
                     NGIEWHSTTVIGARPATGKTLIKDLIINGAFKLNPLMPFRVLEFQFEMLGKNSAIREY
                     SQNLQVSYKYLCSADGVLSDADFERCKLYAKNKLQFPIDVVEESCNVM"
     gene            complement(514..1128)
                     /locus_tag="PK432_02025"
     CDS             complement(514..1128)
                     /locus_tag="PK432_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59736.1"
                     /translation="MGIEVIFNNIAEAKLTPNQFYLLWCINQGITAPLINMHQELRVL
                     TTMEYLIPYPDTNGSFNYQPQPKALSLISKIESYFGVQVKKSNNMLMGQDFEANALKY
                     NEIFPKMKLPSNKPARSPIKEIIVAFREFFKEYDYTWDVIHAAAAYYIEEEEKKGYKY
                     TRTSRYFIRKQDQDKSWISDLAGYCELIKNGEDRDDPKFIEKTF"
     gene            complement(1137..1907)
                     /locus_tag="PK432_02030"
     CDS             complement(1137..1907)
                     /locus_tag="PK432_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOK59737.1"
                     /translation="MSVLDTINIDEVKTKLYENLKPSGWGDKLKSFLLSSDFDKIMAQ
                     LLAEAKEGKRFTPVLKQVFRAFEECPVDKLKVVILGQDPYAQGPQTVREGKDGMVREV
                     SRFPVADGLAFSCSNTNTPQPSLKYIFKALEETVYPNGYTWDPDLKRWSNQGVLLLNC
                     ALTTQLTKTGTHYQIWKPFLSFLFDYLDINHQGTVYAYFGKKAQEWSDNVSDNNPKLI
                     ASHPASAAYQDLERWDCNDIFNKINEQVKKQFNEEIVW"
     gene            1926..2252
                     /locus_tag="PK432_02035"
     CDS             1926..2252
                     /locus_tag="PK432_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59738.1"
                     /translation="MAKIKYQALPKDAIVDIQISGTFYMRMVDLLSALGESVGIDEFK
                     RVLTKLKENNPAESLFEFNVHTLMALIYEVEKQAIAQNKCKEEEMEVPDEPTDSSSKD
                     SSPAVV"
     gene            complement(2209..>2450)
                     /locus_tag="PK432_02040"
     CDS             complement(2209..>2450)
                     /locus_tag="PK432_02040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016043436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOK59739.1"
                     /translation="LKVNLKVSGYTNNQLVHRLVALAFIDNPDDKEQVNHKNGVKTDN
                     RINNLEWVSRSENTKHAIQTGLIQQPEKNLWRNCR"
BASE COUNT          698 a          448 c          392 g          912 t
ORIGIN      
        1 tccattacat tacatgattc ttccactaca tcaataggaa actgaagttt atttttggca
       61 taaagtttac atctttcaaa gtctgcatct gataatacgc catcagcact acacaagtat
      121 ttgtaactta cttgtaaatt ctgagaatat tctcttatag cagagttctt accaagcatc
      181 tcaaactgaa actctaatac ccgaaaaggc attaatggat taagtttaaa tgctccattt
      241 ataattaaat ctttgattaa tgttttacct gttgccggtc tggcacctat tactgtagtg
      301 ctatgccatt caatcccatt tgtagttgca tcattgaatt tttgccacgg agttttgata
      361 cttgtaatct tcccagtttg cctaccttgt aaataagaca aggattctat aaaggcctct
      421 ctctgagtct tccactttgt tggttttgtc atgtgactgc ttgtttatcg cgggataaca
      481 aatatagctt tttacttcta taattgcaag tttttagaag gttttttcaa tgaattttgg
      541 atcatctcta tcttccccat tcttaattaa ttcacagtat cctgctaaat ctgatatcca
      601 gctcttatcc tgatcttgtt ttctaataaa gtaccttgaa gtccgggtat acttataacc
      661 tttcttctcc tcttcttcta tgtaatatgc tgctgctgcg tgtattacat cccaagtata
      721 gtcatattcc ttaaagaatt ctctaaatgc tactataatt tccttgatag gtgatcttgc
      781 cggtttatta cttggcaatt tcatcttagg aaatatctca ttgtacttca atgcattagc
      841 ttcaaagtcc tgacccatga gcatattatt agatttcttt acctgtactc caaaatagct
      901 ttctatctta ctaataagac taagagcctt aggttgaggt tgatagttaa atgaaccatt
      961 tgtatccgga tatggtataa gatattccat agttgttaat acgcgaagtt cttggtgcat
     1021 atttataaga ggtgctgtta ttccttgatt tatacaccat aacagataga attgattagg
     1081 agttaatttg gcttcagcta tattgttaaa tatcacttct atgcccattt tactttttac
     1141 catactattt cttcattaaa ttgtttcttt acttgctcat tgatcttgtt aaagatatca
     1201 ttacaatccc atctttctaa atcttgatat gctgcggatg ctgggtgact ggcaattagc
     1261 tttgggttgt tatctgatac attatcagac cattcctgag ctttcttacc aaagtatgca
     1321 tatacggtac cttgatgatt gatatcaaga taatcaaata gaaaagataa gaagggtttc
     1381 catatctgat aatgggtacc tgttttggta agttgtgttg tgagagcgca gtttagtagt
     1441 aatacgcctt gattagacca tcttttaaga tctgggtccc atgtatatcc atttggataa
     1501 acagtctcct ctaatgcctt aaaaatgtac tttaaagaag gctgtggcgt atttgtgtta
     1561 ctacacgaga atgccagtcc atctgctacc gggaatctac ttacttctct gaccattcca
     1621 tccttacctt ctctgactgt ttgtggtcct tgagcatatg gatcctgacc taggattact
     1681 acctttaatt tatctacagg acattcttca aatgctctaa atacttgttt aagtactgga
     1741 gtgaaccttt ttccttcctt agcttctgct aagagttgag ccattatctt atcaaagtca
     1801 ctgctgagta gaaaactttt tagtttatct ccccagccag atggctttaa attttcatat
     1861 agctttgttt ttacttcatc aatatttata gtatctaata cactcataat ttgtatattt
     1921 gttgtatggc taaaattaaa taccaagctc ttccaaaaga tgctattgtt gacattcaga
     1981 tatcaggaac attctacatg agaatggttg atttattatc cgctttaggt gaatcagttg
     2041 gaatagatga atttaaaaga gtgcttacta agctaaagga aaataatcct gctgaaagtt
     2101 tatttgagtt caatgtgcat actcttatgg cattaattta tgaggtagag aagcaagcaa
     2161 ttgcccaaaa taaatgtaaa gaggaagaaa tggaagttcc tgatgagcct accgacagtt
     2221 cctccaaaga ttcttctccg gctgttgtat aagacctgtt tgaatagcat gttttgtatt
     2281 ttcacttcta ctaacccatt ctagattatt aattctatta tctgttttta caccattctt
     2341 atgattaacc tgttctttat catcaggatt atcaataaag gctaaagcaa caagtctatg
     2401 aactaattgg ttgtttgtat aaccagacac tttaagattt actttgaggt
//
LOCUS       DAOSAB010000090         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_28951_length_2441_cov_2.412406, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000090 DAOSAB010000000
VERSION     DAOSAB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28951_length_2441_cov_2.412406"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /gene="rmuC"
                     /locus_tag="PK432_02045"
     CDS             <1..579
                     /gene="rmuC"
                     /locus_tag="PK432_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOK59740.1"
                     /translation="KINVDAKFPYASLQRMTETDDVDAKKRYMKQFESDIKEKIKQVT
                     TREYISVEDGTVDFVILFIPNEMIFSFIYDKMPQIWEDAMSRKVILAGPFSFTAILRM
                     VKQAYENFRVQENIFKIVGYIQAVEKEFDKFNEEFDKVGDRIEQLQKQYSEVSTTRVN
                     QMRRKMDLVKLEGGTAVNEQMSMLEEGKVEKV"
     gene            609..1190
                     /locus_tag="PK432_02050"
     CDS             609..1190
                     /locus_tag="PK432_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59741.1"
                     /translation="MEDSGVKVIEGRIPVLLSAPHVYSHRRPSLTLSYKWGEEFTDTI
                     VEELCLRSGAWGVIQTKETDYDPNWHKIKENPYKGVIKKIIGDKKFNKFIDIHGLNDE
                     NKYDFGIYYPSRFRRSIVLAEKMRNALDKGELTGVNVCIFRCPDDLQETVAEYVADEL
                     RVPSVQLEIARYIRESDDLRSEFIKNLIPFLKV"
     gene            1316..1612
                     /locus_tag="PK432_02055"
                     /pseudo
     CDS             1316..1612
                     /locus_tag="PK432_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514007.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
     assembly_gap    1613..1622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1623..2279
                     /gene="recA"
                     /locus_tag="PK432_02060"
     CDS             <1623..2279
                     /gene="recA"
                     /locus_tag="PK432_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOK59742.1"
                     /translation="IDELYISQPDFGEQALEILETLVRSAAFGVIVVDSVAALTPRAE
                     IEGEMGDSHMGLQARLMSQALRKITAIAGKTGTTIIFLNQLRMKIGVMFGNPETTTGG
                     NALKFYASIRMDIRQRDKIMRDGNIIGHTRVVKIVKNKLAPPFKEATFDMIYPFGIDK
                     ESSILDAAISTGIVEKSGAWFKFGDKQLAQGRDSTIALLKEDKKLRDEIEKAVRATMK
                     "
BASE COUNT          858 a          287 c          543 g          743 t
ORIGIN      
        1 aagataaatg ttgatgctaa gttcccatat gcatcactgc aaagaatgac tgaaacagac
       61 gatgtagatg ccaaaaagag atatatgaag cagtttgaaa gtgatattaa agaaaagatt
      121 aagcaggtta caacaaggga gtatatttct gttgaagatg gtactgttga ttttgttatt
      181 ctctttattc ctaatgaaat gattttctcc tttatatatg acaaaatgcc tcagatatgg
      241 gaagatgcta tgagcaggaa ggtcatttta gcaggtcctt ttagttttac tgctattctt
      301 agaatggtaa aacaggcata tgagaatttt agggttcaag agaatatttt taagatagta
      361 ggatatattc aagctgttga gaaagagttt gataaattta atgaagaatt tgataaggta
      421 ggagatagaa tagagcaact gcagaagcag tatagcgaag ttagtactac aagggttaat
      481 caaatgagaa gaaaaatgga tcttgtaaaa ttggaagggg gtactgctgt aaatgaacag
      541 atgtctatgc ttgaagaagg taaagtagag aaagtgtaaa aatattctta aatatgttac
      601 tctttaatat ggaagattca ggagtaaaag ttatagaagg aagaatacca gttttgcttt
      661 ctgcaccaca tgtttactct cacagaaggc catctttaac actatcatac aaatggggag
      721 aggagtttac tgatacaatt gtagaagagc tatgtttaag gagcggagca tggggagtta
      781 ttcaaacgaa agaaacagat tatgatccta attggcataa aattaaagaa aatccatata
      841 aaggggtgat taaaaagatt attggtgata agaagtttaa taaatttatt gatatacatg
      901 gtttaaacga tgagaataaa tatgactttg gtatttacta tccttctaga tttcgcagat
      961 caatagtttt agcagagaag atgagaaatg cacttgataa aggagaactt actggggtta
     1021 atgtctgtat attcagatgc ccagatgact tgcaagaaac tgtagctgag tatgtagcag
     1081 atgaattaag ggttccttct gtgcagcttg agattgctag gtatataaga gagagtgatg
     1141 acctaagaag tgagtttatc aagaacttaa taccttttct taaagtttaa aattcattaa
     1201 tatattcagt attttttgac taagatagtt cactttgata tgcttatgaa gagtatataa
     1261 gttaatgtag taaattacta tggtatcaac cgtaaaagag gcaaagaaca gtgagatgag
     1321 tggaaaaagc aaggctattg agagtgctgt tgctcaaatt gaaaaacagt ttggaaaagg
     1381 ttcaataatg aagcttggag gcaaggagag aatagatgtt taagttattc caacaggtgc
     1441 actatcttta gatttagcac ttggtattgg aggtgttcct aagggaagaa ttattgaaat
     1501 atatggacct gagtcatcag gaaagactac acttgcaata catatcctta ctgaagcaca
     1561 aagaaaagga gaagctgttg cttttattga tgcagagcat gcttttgatc ctnnnnnnnn
     1621 nnattgatga gctgtatatc tctcagcctg attttggtga acaggcatta gaaattcttg
     1681 aaacactggt taggtctgct gcttttggag taattgtcgt tgactctgtt gctgcactta
     1741 ctcctcgtgc tgaaatagag ggtgagatgg gtgatagtca tatgggtttg caagccagac
     1801 ttatgtctca agctttacga aagattactg caattgcagg aaagacaggt acaaccatta
     1861 tctttttgaa tcagttaaga atgaagattg gtgttatgtt tggtaatcct gagactacta
     1921 ctggtggtaa tgctcttaaa ttctatgctt caattagaat ggatattaga cagagagata
     1981 aaattatgag agatggaaat attattggtc atacaagggt tgttaagatt gttaaaaata
     2041 aacttgctcc tccttttaaa gaagctacat ttgatatgat ttatcctttt ggtatagata
     2101 aagagagtag catattagac gcagcaataa gcacaggtat tgttgaaaaa tctggagcat
     2161 ggtttaaatt tggagacaaa cagttagcac agggtagaga ttctacaata gcactgctta
     2221 aagaagataa aaagctaaga gatgagattg agaaggctgt tcgtgctaca atgaagtaat
     2281 ggtaattaca aagattgaat atcagaaaag agatcccgag agggtaaata tatatattga
     2341 tggaaaattc ttctgcggaa tatccattga tacacttgct ttggagaatt tgtatgaagg
     2401 cttagaggta gatgataatc ttttagataa aatattaaag g
//
LOCUS       DAOSAB010000091         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_29030_length_2436_cov_2.858883, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000091 DAOSAB010000000
VERSION     DAOSAB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29030_length_2436_cov_2.858883"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PK432_02065"
     CDS             complement(<1..933)
                     /locus_tag="PK432_02065"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOK59743.1"
                     /translation="MDNNEEKLEKMRHSASHVLAQAVLKLYPDTKLGIGPAIENGFYY
                     DFEFSQPIEEKDLKPIEEEMKKIIKRKLPITQIFMKRKDAIKYLKDIGQEYKLELLEE
                     IPDKELSFFVTGQQEFVDLCRGPHVANTGEIGAIKLIKTAGAYWKGDENRKMLTRIYG
                     AAFETKAELKKYLQDLENAEKYNHRALGKQLKLFAIIPEIGQGLPVWLPRGYTMRRIL
                     ENYMFDLERAHGYQHILTPHIVREELFKISGHLNFYKDSMYAPIKVDEENYYLKPMNC
                     PEGMMVYKMETRSYRDLPLKLGEFGTVYRYEKTGE"
     gene            complement(1106..1843)
                     /locus_tag="PK432_02070"
     CDS             complement(1106..1843)
                     /locus_tag="PK432_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOK59744.1"
                     /translation="MSLKFDSSKKLRKEKSKHKVESWEEYEQREQQFLRERYLSKEPT
                     ISTFVRITAGKAKNFKIEIPRNTRPLTDRMKVRIFDVLGSDIVNKSILDLYAGAGSFA
                     LEALSRGAKEATLVDASKQGNIIIQRNVAHTGFLPQAEVIKSKVEDFIQKYKDSDKTW
                     DIIFMDPPYKLYNTKRVEKMERIINDASSLLPGVKNPKTKKFKGAFIIKHPKRYPIDK
                     LDLKHIKKVDTYEFGLNSISLFIVKSK"
     gene            complement(1857..>2436)
                     /locus_tag="PK432_02075"
     CDS             complement(1857..>2436)
                     /locus_tag="PK432_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59745.1"
                     /translation="KTAITPITNGSRSTDISYAGTTLSLNGTTYYWRIRFWDNKGAVS
                     NWSQTASFTMSGNPFSPTYLKTDGMENPTWILSTTPKFSALHTDPNSDSANYYEIEVN
                     SNSSFTGTVMWDTGKVSMTSTSSGSRSPEITYAGTALTGTSGTTYYWRIRFWDTDGNV
                     SNWSDTATFKDFIQTEEYIQMEKLKLEGIKIN"
BASE COUNT          649 a          433 c          354 g         1000 t
ORIGIN      
        1 ttctccagtt ttctcatatc tgtatacagt accaaattca cctaatttta acggtaaatc
       61 tctgtagctt cttgtttcca ttttgtatac catcattccc tctggacagt tcattggttt
      121 aaggtagtag ttctcttcat caactttaat tggagcatac atggagtctt tgtaaaagtt
      181 tagatgacca gatattttaa atagctcctc tcttacaatg tgaggagtta gaatatgctg
      241 atacccatgt gctctttcaa ggtcaaacat atagttctca aggattcttc tcattgtata
      301 tcctcttgga agccataccg gaagtccttg cccaatctca gggataattg caaatagttt
      361 tagctgcttt cctaatgctc tgtgattata cttttctgca ttctctaaat cttgcaaata
      421 ttttttaagc tctgcttttg tttcaaatgc tgctccatat attcttgtga gcatctttct
      481 attctcatcc cctttccaat atgcaccagc tgtttttatt aatttaatag cccctatttc
      541 accagtattt gctacatgag gacctctgca tagatctaca aactcttgtt gccctgttac
      601 aaagaaacta agctctttat caggtatttc ttctaaaagt tccaatttgt actcctgtcc
      661 tatatcttta agatacttaa ttgcatcttt tcttttcata aagatctgag ttattggcaa
      721 ctttcttttt atgatttttt tcatttcttc ttcaatcggc tttagatctt tttcttcaat
      781 aggttgagag aattcgaaat catagtaaaa gccgttttct attgcaggac ctattcctaa
      841 tttggtatcg ggatagagtt ttaatactgc ttgagcaagt acgtgtgaag cagaatgtct
      901 cattttttct aatttttctt cattattatc catttaagta attatacaaa attaatatat
      961 gctatatttc tgggaaaatg tttgcatgaa aagtttaaca ctaaaggcat gttgagtata
     1021 ctaccttatg aattttagat ttattaatac caaatttctt atacatcttc tttattctat
     1081 atcttttatt taaaatatac aattgttatt ttgactttac tataaacagg gatatggaat
     1141 ttaaaccaaa ttcatatgta tctacctttt taatatgttt taggtctagt ttatcaatag
     1201 gatatctttt aggatgttta attataaaag cacccttaaa tttcttagtc ttaggatttt
     1261 taacgccagg aagaagagat gatgcatcat taattattct ttccatcttc tctactcttt
     1321 ttgtattgta aagtttatat gggggatcca taaatataat gtcccatgtc ttatcactat
     1381 ctttatattt ctgtataaag tcctctactt tactcttaat tacctctgcc tgaggaagaa
     1441 atcctgtatg agctacattc ctttgtatta taatatttcc ttgttttgaa gcatctacta
     1501 aagttgcctc tttagcacct cttgagagtg cttctagtgc gaatgaacct gctcctgcat
     1561 aaagatctag aatacttttg tttactatat cacttcctaa tacatcaaat attcttacct
     1621 tcattctgtc agtaagtggt cttgtatttc ttggtatttc tattttaaaa tttttcgctt
     1681 ttcctgcagt aattcttaca aatgtagaaa tggtaggttc tttactaaga tatctttctc
     1741 ttaagaactg ttgttctctt tgttcgtact cttcccagct ttctacttta tgcttacttt
     1801 tttcttttct taatttttta gatgaatcga attttaaact catgatgtgt attttactag
     1861 tttatcttta ttccttccag ttttaatttt tccatttgta tatactcttc agtttgtatg
     1921 aagtctttaa atgttgctgt atctgaccag ttgcttacat ttccatcagt atcccaaaat
     1981 ctaattctcc agtaatatgt tgtacccgaa gttcctgtta atgcagttcc tgcatatgta
     2041 atttcagggg atcttgatcc acttgatgta gatgtcatag atactttgcc tgtatcccac
     2101 attacagttc ctgtgaaaga tgagttagag ttaacttcta tttcatagta gtttgcacta
     2161 tcactatttg ggtctgtatg tagtgctgaa aatttaggag ttgttgataa tatccatgta
     2221 ggattctcca taccatctgt ttttaagtaa gttggagaga aaggatttcc tgacatagta
     2281 aaacttgctg tttgtgacca attgcttaca gctcctttat tatcccaaaa tcttattctc
     2341 caatagtagg ttgtaccatt taaggatagt gttgttcctg catatgagat atctgtagat
     2401 cttgatccat tggtaatcgg ggtgatagct gttttt
//
LOCUS       DAOSAB010000092         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_29152_length_2428_cov_2.960388, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000092 DAOSAB010000000
VERSION     DAOSAB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29152_length_2428_cov_2.960388"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..1403
                     /locus_tag="PK432_02080"
     CDS             162..1403
                     /locus_tag="PK432_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59746.1"
                     /translation="MKKIKFKFKKINWKGIFNYFISVVISALILALILISLPLTEKVS
                     SSASYDLVNKNNMYWAKEYTLELDISQSSDIEKDITQLKSVLQKRLKQFDVEQSSITS
                     YKENDKYYLKVNVQTSKEQELVEQLVKAPFIVTIVTRKDDVNYEDTENPLIPYLEENY
                     NKTEFTRKDFRNVYVTKLKNTSGEYSYFAIFKAWPWSSKWNEFLKKYSGQIVGVSIDG
                     FVTPVQIIEGDKTFAPSTSATDEKYAKVISILYNSGVAPMSYVVTNEEEKPLDIAEID
                     YVKLTEGILIAILFIYVYLLVVEKTRKSIILKSGIATVLTISSWIAYLKLSATPVDIF
                     ILALEVIVVAAIIRMITENTESRVHITLLLTFMSVLCILFGFGYVKIFANDLLILIIL
                     SNLSLLISTYYISKVRKSLKV"
     gene            1400..1912
                     /locus_tag="PK432_02085"
     CDS             1400..1912
                     /locus_tag="PK432_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59747.1"
                     /translation="MNLIQNVIQNWSRYELRTEIFLYLSIIAIAVLTIYLFSKNGKIT
                     LLSFISLCILLLLNFLGFIIISTLLKIQITEVFRMTPVISMVFLISNLGLLTGFYSSK
                     KKMKEIKFANIRKEYLSDSIKESVFLILLGISTIFFLSPATEIVVSMSILASIAAIWL
                     TYWISKYILK"
     gene            1909..>2428
                     /locus_tag="PK432_02090"
     CDS             1909..>2428
                     /locus_tag="PK432_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HOK59748.1"
                     /translation="MIKYKGKIIVIAGATATGKSEISLKLAKKINGYIINADSRQIYK
                     KINIGSAKPKFDKEISKGVHTLNGITHYLYDFVDPKENFTIFDYQNCVNNVLKNTQGI
                     PILTGGSGLYIDSVIYNYKLSKNNKNTDLKDKSIEELQELAKEYLPYMSESDRKNKHR
                     LIRAIQREGVNRE"
BASE COUNT          981 a          363 c          371 g          713 t
ORIGIN      
        1 gcagttagca gtgttgaaaa agagcttggc agatcaataa gatatactgt aatgaaagta
       61 gaagattttg aatttggaaa gaagaaaaga gatccaatat taatgaatgc tctcatgaaa
      121 gataagataa tactaatagg taaagactca gatttactgt aatgaaaaag ataaaattca
      181 aatttaagaa aataaactgg aaaggtattt ttaactattt catttcagta gtaattagtg
      241 ctctcatact tgccttaata cttatttcac tcccgcttac agagaaagta tcttcctctg
      301 catcatatga ccttgtaaac aaaaataaca tgtactgggc aaaagagtat acacttgaac
      361 ttgatatctc acaaagcagt gatatagaaa aagatattac acagcttaaa tcagtacttc
      421 aaaaaagact taaacaattt gatgttgaac aatcatcaat tacatcatac aaagaaaatg
      481 acaaatacta tcttaaagta aatgtacaaa catctaaaga gcaggagtta gttgaacagc
      541 ttgttaaagc tccctttatt gtaaccatcg ttacaagaaa agatgatgta aattatgaag
      601 atacagaaaa tcccctaatt ccttaccttg aagaaaacta taataaaaca gagttcacaa
      661 gaaaagattt tagaaatgtt tatgtaacaa agctcaaaaa tacaagcgga gagtattcat
      721 actttgcaat atttaaagca tggccgtgga gtagtaaatg gaatgaattc ttaaagaagt
      781 attcaggaca aatagtggga gtaagcattg atggctttgt aacacctgtc cagataattg
      841 aaggagataa aacatttgca ccatctactt ctgctacaga tgagaaatat gcaaaagtaa
      901 taagcatatt gtacaacagt ggtgttgcac caatgtcata tgtagtaaca aatgaagaag
      961 aaaaacctct tgatatcgct gaaatagatt atgttaaatt aacagaagga attctaattg
     1021 caatactctt tatatatgta tacctattgg tagtagagaa aacaagaaag agtataattc
     1081 ttaaatcagg tatagcaaca gtactaacaa tatcctcatg gatagcatat cttaaactat
     1141 cagcaacacc agttgacata tttatacttg cattagaagt aatagtagta gcagcaataa
     1201 ttagaatgat aacagaaaac acagaatcaa gagttcacat tacactgctt cttaccttca
     1261 tgtcagttct gtgtatatta tttggttttg gttatgtaaa aatatttgca aacgatttac
     1321 taattctcat catactaagc aatctttcac tcctaatttc aacctactat atatctaaag
     1381 taagaaaatc tctaaaagta tgaacttaat acaaaatgta atacaaaatt ggagcagata
     1441 tgaattaaga acagagatat ttctgtatct ttccattatt gcaatagcag tcctaactat
     1501 atatctattt tcaaagaacg ggaaaatcac tctcctttca ttcatatccc tatgtattct
     1561 tctcctttta aacttcctcg gctttattat tatcagcacc cttcttaaaa tacaaattac
     1621 agaggtattt agaatgacac cagtaatttc aatggtattc ttaatttcta acttaggtct
     1681 tcttacaggc ttttactcat caaaaaagaa aatgaaagaa attaaatttg ccaacattag
     1741 aaaagaatat ctgtcggata gcataaagga gagtgtattc ttaatactgc tgggaatatc
     1801 tacaatattc ttcctctctc ctgcaactga aatagttgta tcaatgtcaa tattagccag
     1861 tatagctgca atatggctta catactggat atctaaatac attctcaaat gattaagtac
     1921 aaaggaaaga taattgtaat agcaggagct actgcaacag gtaagtctga aatatctctt
     1981 aaactagcaa agaagataaa tgggtatatt attaatgcag attcaagaca gatatacaaa
     2041 aaaatcaaca ttggaagtgc aaaacctaaa tttgacaagg aaatctctaa aggtgttcat
     2101 actctcaatg gtattactca ctacctttat gattttgtag accccaaaga aaactttact
     2161 atttttgatt atcaaaattg tgtaaataat gtacttaaaa atacacaagg aatacccata
     2221 cttacaggag gaagtggatt gtatatagat agtgtaatat acaactacaa attaagtaaa
     2281 aataacaaaa atactgattt aaaagataaa agcatagaag aactacaaga attagcaaaa
     2341 gagtacttac catatatgag tgaaagcgat agaaagaaca aacacagatt gataagagca
     2401 atacaaagag aaggagtaaa cagagaaa
//
LOCUS       DAOSAB010000093         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_29553_length_2403_cov_2.132879, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000093 DAOSAB010000000
VERSION     DAOSAB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29553_length_2403_cov_2.132879"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PK432_02095"
     CDS             <1..573
                     /locus_tag="PK432_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOK59749.1"
                     /translation="STLRVLGVDDKNMNTLLKDFSSVLGRMNVLQKEPFMIIVDFAHT
                     PNALESALESASKLKKKNSKIILVFGCASKRDDYKRPIMGEYAKKYADITILTAEDCR
                     MESLKKINDEIEKGWKEYQTNEKRQLIRFDDDTHNIKVRRDAIKKAISLASSGDIVLI
                     TGKGHERSLCFGLKEYEWSDIAEIKKLIRR"
     gene            593..>2403
                     /gene="mutS"
                     /locus_tag="PK432_02100"
     CDS             593..>2403
                     /gene="mutS"
                     /locus_tag="PK432_02100"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOK59750.1"
                     /translation="MEFDIKQTPMMDQYLKFKKQYPDKIVLFRMGDFFETFGQDARIT
                     SKVLNITLTSRDKNSNPTPLAGFPHKAIDQYLPKLVHAGYCVVIVDQLEDPRLAKGIV
                     KRGVTRIVTPATIDDSEAIKSSYMCSFVKLKNDLTACVCDMSTGDLFWIYTKYSEKSI
                     ESILSSFDPVEILLLEDEKELKFSSLPVQFLTKGLKNIKYCEELIKDFFKVSDMHSLG
                     LEDNVAAVCAVAMVLYYIQDTQLMEPAHIKKPKRKNLNSSMVLDRATIKNLELVASSY
                     TGLVSGSLFSVIDGTSTQMGKRMLYSWVLNPLIDKKDIDGRLDIVELFFNNREALSKT
                     KELLSNVNDISRIAGKIGLRRANGRELKGLQISLENISKLRTILLEIPEILKELDKYD
                     SLLNEVIKKIDDCIVDAPPLTLLEGGIIKSSYNSEIKELRELSGDSKGWIKEFEEKEK
                     KSTGISSLKIGFNKVFGYYIEVTKAHQEKVPERYIRKQTLVNSERYITEELKQKEDII
                     LNAQERLNELEYELFTEFRDSLMKYIPELQSLSEYISEIDILSGFAKIAIERDYCKPV
                     IYNMGEENGIIEIKSGRHPIVEILNEGEFISNDTYID"
BASE COUNT          910 a          271 c          478 g          744 t
ORIGIN      
        1 tcaacattaa gagttttagg agtagatgat aaaaatatga atacactttt aaaagatttt
       61 tcttctgtgc tgggaagaat gaatgtttta caaaaagagc cctttatgat aatagttgat
      121 ttcgcacata ctcctaatgc actagagagt gctttagaga gtgcaagtaa gcttaagaaa
      181 aagaactcta aaattatact tgtatttggt tgtgcatcca aaagagatga ttacaaaaga
      241 ccaattatgg gggaatatgc aaagaaatat gcagatatta caattttgac tgctgaagat
      301 tgtagaatgg agagtttaaa aaagataaat gatgaaattg agaaaggatg gaaagagtat
      361 caaacaaatg aaaaaagaca attaattaga tttgatgatg atacacataa tattaaggta
      421 cgcagagatg ctatcaagaa agccatatcc ttagctagca gtggagatat tgttttaata
      481 actggaaagg ggcacgaaag aagtttatgt tttggtttga aagagtatga atggagtgat
      541 attgctgaga taaaaaaact gataaggaga taattctatt gtataataac atatggaatt
      601 cgatattaaa caaaccccaa tgatggatca gtacttgaaa tttaagaagc agtatcctga
      661 taagatagtg ctttttagaa tgggagattt ttttgaaaca tttggacaag atgcaagaat
      721 aacatctaag gtacttaaca ttacacttac ttcaagggat aagaacagta accctactcc
      781 acttgcagga tttcctcata aggcaattga tcagtatctt cctaaacttg ttcatgctgg
      841 gtactgtgta gttattgttg atcagcttga agatccaagg ttagcaaaag gtattgtaaa
      901 aagaggagtt acaagaatag ttaccccagc tactattgat gattcagaag ctataaaaag
      961 cagctatatg tgctcctttg ttaagctaaa aaatgattta acagcttgtg tttgtgatat
     1021 gtctactgga gatttatttt ggatatatac gaaatatagt gaaaagagta ttgagagtat
     1081 tctttcttct tttgatcctg ttgagatact tcttttagaa gatgaaaaag aattgaagtt
     1141 ctcttctctc cctgttcagt ttttaacaaa gggacttaag aatattaaat attgtgagga
     1201 gctgattaaa gatttcttta aggtgtctga tatgcactcc cttggtttgg aagataatgt
     1261 agcagcagta tgtgctgttg caatggttct ttattatatt caagatactc aactaatgga
     1321 gcctgcacat attaaaaaac caaaaagaaa aaatcttaat tcaagtatgg tattagatag
     1381 ggcaacaata aagaatttag agttagtagc aagctcatat acaggtttag tatctggttc
     1441 tcttttttct gtgatagatg gtacttcaac acaaatggga aagagaatgc tctattcatg
     1501 ggtactaaac cctttgattg ataaaaagga tatagatgga agattggata ttgttgagtt
     1561 attttttaat aatagagaag cactaagtaa aacaaaagaa ctgctttcaa atgtaaatga
     1621 tattagtaga atagctggaa agatagggtt aagaagagcg aatggaagag agttaaaagg
     1681 gttacagata tctttggaga atatatcaaa attaagaact attctgttag aaatccctga
     1741 aatattaaaa gagcttgata aatatgattc tctcttaaat gaagttatta aaaaaataga
     1801 tgattgtatt gtagatgctc ctcctcttac acttcttgaa ggaggtatta ttaaatcttc
     1861 atacaattct gaaattaaag agttaagaga gctaagtgga gatagtaaag gatggataaa
     1921 agaatttgaa gaaaaagaga aaaagagtac agggattagt agtcttaaaa ttgggtttaa
     1981 taaagtgttt ggctattata ttgaagtaac taaagctcat caagagaaag ttcctgagag
     2041 atatataagg aaacagacat tagttaatag tgaaagatat ataacagaag agcttaaaca
     2101 aaaagaggat attatactaa atgcccaaga gagattaaat gagttagaat atgagctgtt
     2161 tactgagttt agagactctt taatgaaata tataccagag cttcaaagtt tgtccgaata
     2221 tatttccgaa attgatatac tctctggatt tgcaaaaatt gcgatagaaa gagattactg
     2281 taaacctgtt atttataata tgggagaaga gaatggaata attgagataa aatcaggcag
     2341 acatcccatt gttgaaatct taaatgaagg tgagtttatt tctaatgata catatattga
     2401 tta
//
LOCUS       DAOSAB010000094         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_30116_length_2370_cov_3.086393, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000094 DAOSAB010000000
VERSION     DAOSAB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30116_length_2370_cov_3.086393"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PK432_02105"
     CDS             <1..689
                     /locus_tag="PK432_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59751.1"
                     /translation="AGKELLLLIPIAIFIFLNLSLYGGNLLKYKTLEPSCTQILTHEQ
                     CLENGVYYRNKVNYSATPINGPLDVFNMIIKGERIGPVRYFFRWFPNLMSKIFGIMGD
                     SSLSLPYYLVALFILFFLTGVVLIISNKKRLTKIDKYLLTILLFYTSILFFTQNYPMY
                     LTYNHYYLGLQGRYIFPVISIIYILLTKSFFFIKPKKLGNILLISFSILLIYSCNIYL
                     FLNLPNSWLK"
     gene            763..1938
                     /locus_tag="PK432_02110"
     CDS             763..1938
                     /locus_tag="PK432_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOK59752.1"
                     /translation="MRYIGTCVRGIRTPVIKNGDNLEEVVVSSLLRASKAHNFEFNDK
                     DVVGITEAVVAITQGNYVNLEQLTTEIKKKFPKDIGVVFPILSRNRFSLILTAIANAC
                     SDVHILLSYPSDEVGNHLMNEEKMFEVGLNPCSDSFEEEKYRALFEKKDVLHEFTGMD
                     YVEYYKSLGHGNIHIHFSNDPKYILKFSKNILVCDIHTRFRTKKVLQKTGVDSIYSLA
                     DICSTPSRVHGFNPEYGLLGSNKVGEDKLKLFPRDCYAIVNNIQKRLKKETGKNIEVM
                     VYGDGAFKDPVGGIWELADPVVSPGFTDGLLGRPSEIKLKFVSENWNKQGSLDEYVKE
                     AIRQKKGPDYQVEKSLGTTPRQLTDLLGSLCDLTSGSGDKGTPVILIQGYFDDYTKE"
     gene            1963..>2370
                     /locus_tag="PK432_02115"
     CDS             1963..>2370
                     /locus_tag="PK432_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59753.1"
                     /translation="MKKKHIIFLVLLLAFGIFLYLFITRSSKDATEYVDVSQSEMSAF
                     IFVTKESETNHVLNIYLTPFLKFNAEEKIKEFSITNFNGHNDISQVLLVPPTTLSIDT
                     PAKTFLFTVDENIVQDDIRYIADGIEYDVSDSVK"
BASE COUNT          790 a          291 c          434 g          855 t
ORIGIN      
        1 aggctgggaa ggagcttctg ctgttaatac caattgcaat ttttatattt ttaaatctat
       61 ctttgtacgg aggcaatctt cttaaataca aaacccttga gcctagctgt acacagattc
      121 taactcatga gcagtgttta gagaatggtg tatattacag aaataaagtg aattatagtg
      181 ctacccctat taatggtcct ttagatgttt ttaatatgat aataaaagga gagagaatag
      241 gacctgtaag atattttttt agatggtttc ctaatttaat gagtaagatt tttggaatta
      301 tgggagatag tagtttgtct cttccctatt atttagttgc actttttata cttttctttt
      361 taacaggagt tgttttgata atttctaata aaaagagatt gacaaagata gataaatatt
      421 tgttaacaat acttctcttt tatacttcta ttcttttctt tacacagaac tatcctatgt
      481 atttaacata caatcattac tatttaggtc ttcaaggaag atatatattt cctgtaatat
      541 caataatata tatactttta actaaatcat ttttctttat taaacccaaa aagttaggaa
      601 atattctttt aatttctttt tctatccttt taatatactc ttgtaatata tatcttttct
      661 taaatcttcc aaactcgtgg cttaagtaat gtttatttcc tggacatggg tttatagata
      721 gtttactatt gatatggaga aataattttt taaagtaaaa acatgagata tattggaacg
      781 tgtgtaagag gtattagaac accagttatt aaaaatggag acaatttaga ggaggttgtt
      841 gtctcatctc ttcttagagc atcaaaggct cataattttg aatttaatga taaagatgta
      901 gttggtatta cagaggcagt tgttgcaatt actcagggca attatgttaa cttagaacag
      961 ttgactactg aaattaaaaa gaagtttcca aaagatatag gggtggtttt tccaatcctt
     1021 agtagaaaca gattttcttt gatacttact gctattgcta atgcatgcag tgatgtacac
     1081 attcttttaa gttatccatc tgatgaggtt ggaaatcact taatgaatga agaaaaaatg
     1141 tttgaggtag ggcttaatcc gtgttctgat tcttttgagg aagagaaata tagagctctt
     1201 tttgagaaga aggatgtttt gcatgagttt acaggtatgg attatgttga gtattacaag
     1261 agcttagggc atgggaatat tcatattcat ttttcaaatg atccaaaata tatcttaaaa
     1321 ttttcaaaaa atatattagt ttgtgatatt catacaagat ttagaacaaa gaaagtttta
     1381 cagaagactg gtgtagatag tatatattct cttgcagata tatgctctac tccatcaagg
     1441 gtgcatggct ttaatccaga gtacgggctt ttaggatcaa acaaggtagg agaagataag
     1501 ttaaaactgt ttcctcgaga ttgttatgct attgttaata atattcaaaa aagattaaag
     1561 aaggaaacag gtaaaaatat tgaagtaatg gtttatggag atggggcatt taaggatcct
     1621 gttggtggta tttgggagct tgctgatcct gttgtttctc ctggttttac agatggatta
     1681 cttggtagac caagtgagat aaagttgaaa tttgtatctg agaattggaa caaacagggg
     1741 agtttagatg agtatgttaa ggaggcaata cgacaaaaga aaggaccaga ttatcaagtt
     1801 gaaaaatctc ttggtacaac tccaaggcag ttaactgacc ttttgggtag tctatgtgat
     1861 cttacaagtg gaagtggtga taaaggaacg cccgttatct taatacaggg atattttgat
     1921 gactatacaa aagagtaatt gtaaaattaa aatataaata gtatgaagaa aaaacatatc
     1981 atattccttg ttcttcttct tgcttttggc atctttcttt atttatttat tactagaagc
     2041 agtaaagatg ctactgagta tgttgatgta tctcaatctg aaatgtctgc attcatattt
     2101 gttactaagg aaagtgaaac taatcatgtc ttaaatatct atttaacacc tttcttaaag
     2161 tttaatgcag aagaaaaaat caaagagttt agtattacaa actttaatgg gcataatgat
     2221 atttctcagg ttttgcttgt tcctcctact actttgtcta tagatactcc tgctaaaaca
     2281 tttcttttta cagttgatga gaatatagta caagatgata ttagatatat agcagatggt
     2341 atagagtatg atgtgagtga tagtgttaaa
//
LOCUS       DAOSAB010000095         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_30783_length_2332_cov_3.247255, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000095 DAOSAB010000000
VERSION     DAOSAB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30783_length_2332_cov_3.247255"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /gene="gpmI"
                     /locus_tag="PK432_02120"
     CDS             <1..1260
                     /gene="gpmI"
                     /locus_tag="PK432_02120"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOK59754.1"
                     /translation="DFANKNKSRIHILGLASQGGVHADTGHITATIEFFSKRNFQGNI
                     FIHAFTDGRDSPVNDAMRNLNIIQKYIENNGIGQIATLCGRAWAMDRNKLWDRTKKAY
                     DLLVSNIGQECNSYINCLQNAYSQNISDEYIPPTVLVKNSNIRKGDVVLFLNFRPDRA
                     LQLSEALYEEVFDKFPRQDISPIFFAGMVEYKRGFPKKVIFPKQYISLPIGNIFSSYG
                     YRQLRIAESEKFPHVTYFFNGGVEVKYPGEDRIEVPSPSVPTYDLQPEMSAIKVTEIL
                     IDRIQKNIYDFLLVNFANPDMVGHTGNISATIKAISTVDYCVNRLVKEFTARNGVVII
                     TADHGNAEELINIDTNVIDTEHSFNPVPLIIAGLPTFTNKLKYGSLKDIAPTILDIME
                     IPIPSEMTGKSLLYSSTSPAFNSADDV"
     gene            complement(1207..1836)
                     /locus_tag="PK432_02125"
     CDS             complement(1207..1836)
                     /locus_tag="PK432_02125"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOK59755.1"
                     /translation="MIQSIIDWLVNAISTIGYPGVFISVFLESFFAPIPSEIILPFSG
                     FVASTGKMNLIFVIVIATVAAYLGSLPFYFIGRWGEKPVINFINKYGKYLFIQQKDVD
                     KVFGAFDKYGKGVVFFGRLIPMIRTLISFPAGVAKMEFARFSMYTLIGSLTWNILLTY
                     AGYQLGDHWSVVSGWIKKYQNVILILIAIALILYIIRGIKSRRCTGVKK"
     gene            complement(1867..>2332)
                     /locus_tag="PK432_02130"
     CDS             complement(1867..>2332)
                     /locus_tag="PK432_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOK59756.1"
                     /translation="IQNRYQLFEISFDNTKEIKKEEVKKDENKEQSIEILNSEGKRIH
                     INVEIADTPSLKSQGLSGREDIGDYQGMLFLFDEKGVYSFWMKDMNFPLDLIYIDETY
                     YIVDIIENIQPCTQNECNSFVPSVPFKYVLEVKGGFCDVNRVSIGNAVIFDI"
BASE COUNT          856 a          406 c          342 g          728 t
ORIGIN      
        1 gattttgcaa ataaaaataa atctaggata catatacttg gtttagcaag tcagggaggt
       61 gtacatgccg atacaggaca tatcacagca actattgagt ttttttcaaa gcggaatttt
      121 cagggaaaca tttttataca tgcctttact gatgggagag attctcctgt taatgatgct
      181 atgagaaatc ttaatattat tcaaaagtac atagaaaata atggaatagg acaaattgct
      241 acactgtgcg gtagagcatg ggcaatggat agaaacaaat tatgggatag aacaaaaaaa
      301 gcatacgact tacttgtatc aaatattgga caagagtgta attcatatat taattgcctg
      361 caaaatgcat actcccagaa tatttcagat gaatatattc cccctactgt acttgtaaag
      421 aatagtaata ttagaaaagg agatgttgtt cttttcttaa atttcagacc cgatagagca
      481 ttacagctat cagaagccct gtatgaagaa gtttttgata aatttccaag acaggatata
      541 tctcctatat tttttgcagg aatggtagag tacaaaagag gatttcccaa gaaggtaata
      601 tttcctaagc agtatataag tttgccaatt ggaaatattt tttcatcata tggctacaga
      661 cagctaagaa tagcagaatc agaaaagttt cctcatgtta catacttctt caatggaggg
      721 gtagaagtta aatatccagg ggaagataga attgaggtac cttctccatc tgttcctaca
      781 tatgatcttc aacctgaaat gagtgctatt aaagtaacag aaatccttat agatagaatt
      841 caaaaaaaca tatatgattt tctcttagtc aacttcgcta atcctgacat ggtaggacac
      901 acaggaaata tctctgcgac aattaaagcg atatcaacag tagattattg tgtaaataga
      961 ttagtaaaag agttcacagc aagaaatggt gtagtaataa ttaccgctga ccatggaaat
     1021 gctgaggaat taataaatat tgatacaaat gtgatagaca cagagcactc ctttaatcca
     1081 gtcccattaa ttattgctgg attaccaaca tttacaaaca aattaaaata tggatcacta
     1141 aaagatattg ccccaacaat acttgatatt atggaaatac caataccaag cgaaatgaca
     1201 ggaaaatcac ttctttactc cagtacatct cctgctttta attccgcgga tgatgtataa
     1261 aataagtgcg atagctatca aaattaatat tacattctga tattttttta tccacccgct
     1321 tactacactc cagtgatctc ctaactgata tcctgcatat gtcaaaagaa tattccatgt
     1381 taaagatcca atcaatgtat acatactaaa tcttgcaaat tccatttttg caacacctgc
     1441 aggaaatgag ataagagttc gtatcatagg aataagtctt ccaaaaaata caactccttt
     1501 tccatattta tcaaatgctc caaacacttt atctacatcc ttttgttgaa taaaaaggta
     1561 tttaccatat ttattaataa aattgataac aggcttttca ccccacctgc cgatgaagta
     1621 aaaagggaga gaacctaaat atgcagcaac tgttgctata acaattacaa aaataagatt
     1681 cattttacca gttgaggcaa caaaacctga aaaaggtagt attatttcac taggtatagg
     1741 tgcaaaaaaa ctttcaagaa atactgaaat aaagacacca ggataaccaa tggtagaaat
     1801 agcatttaca agccaatcaa ttatactttg aatcataagt acaatctttg aagttaattc
     1861 ttagaatcat atatcaaata ttacagcatt tccaatactt accctattaa catcacaaaa
     1921 accaccttta acctctagga catatttaaa aggaacagaa ggaacaaagc tattacactc
     1981 attctgtgtg catggttgta tgttttcaat aatatctact atgtaatatg tttcatcaat
     2041 atatattaaa tcaagaggaa aattcatatc tttcatccaa aatgaatata cacccttttc
     2101 atcaaaaaga aacaacatac cttgataatc ccctatatcc tcccttcctg aaagcccctg
     2161 agatttgagt gatggtgtat cagcaatttc aacattaata tgtattcttt ttccctcact
     2221 atttaatatt tcaatacttt gctccttatt ctcatccttt ttaacctctt cttttttaat
     2281 ttctttggta ttatcaaatg atatttcaaa caactgatac ctattttgaa ta
//
LOCUS       DAOSAB010000096         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_31166_length_2311_cov_3.479610, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000096 DAOSAB010000000
VERSION     DAOSAB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31166_length_2311_cov_3.479610"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PK432_02135"
     CDS             <1..693
                     /locus_tag="PK432_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOK59757.1"
                     /translation="HLQDNNYTPKSISRKINSTRTFYRFLLERSVVTDNIAEKLAHPK
                     FETKPPRVLSEMEYRALRDVSRGDIRLYSIVEVLLQTGIRIGELATLTIDDIRRSKEG
                     VDFLYIKASGSHPARKVPLNKSAKKAIDAYLEIRPETEDDTLYVTKNGRPLLVRNIRT
                     AIDKAFEKASIKNAKVNDLRNTFIAHHLAKGVSLVTVSKLVGHKRISTTEKYLGLVTD
                     NRPEAEKLLKEL"
     gene            complement(730..1065)
                     /locus_tag="PK432_02140"
     CDS             complement(730..1065)
                     /locus_tag="PK432_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59758.1"
                     /translation="MDKQRVLMVIFVVWVILLNIIFIFFWKATNLADFVILERISPLV
                     CKMEEYKYKIPYSKLSKSNISESEWSVYQKVGNELIKENPCPKYEDYVWFNLIEESLS
                     STFDEFIEK"
     gene            complement(1088..1163)
                     /locus_tag="PK432_02145"
     tRNA            complement(1088..1163)
                     /locus_tag="PK432_02145"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1128..1130),aa:Ile,seq:tat)
     gene            complement(1207..>2311)
                     /locus_tag="PK432_02150"
     CDS             complement(1207..>2311)
                     /locus_tag="PK432_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HOK59759.1"
                     /translation="FERPFGLNILEFYNEQHKHQIVNSFIDLLIRLFDPHNQGYVGPM
                     MQQAVRNSMLTAMSVQGSTLIEVVRMLQDEKWVYEKWLPLVKDELVRRYWTDQVAKTD
                     AKTKSESLGYFISKFDKFTTNLAIRNIIGQSKSSFDFREVMDTGKILIVNLSKGALGE
                     ENMSFLGLLLVQKLLAAALSREDIPEGERKDFFFYADEFQNFATQEFASILSEARKYR
                     LNLTLAHQYIGQLPEDIKNAVFGNVGSLFITRCSNEDAQFLEPQFETYVKATDIINQG
                     LAHYYVKMLTNNSYPSPFSLDARYGPKYVNETGFDLKVNPEVKDIIAKMSRLKYGRDI
                     NLVNMEINRRADLDRVENESAQNNPSGFPPIDF"
BASE COUNT          824 a          420 c          357 g          710 t
ORIGIN      
        1 cacttacagg acaataacta tactcccaag agtatatcta gaaaaataaa tagtacaaga
       61 actttttata gatttttact tgagcgttct gtagttactg ataacattgc agagaaactt
      121 gcacatccta agtttgaaac aaaacctcct agagtactct ctgagatgga gtatagggca
      181 ctgcgagatg taagcagagg agatattaga ctttattcga ttgtagaagt acttcttcaa
      241 acaggtataa gaatcggaga gctagcaact ctaacaattg atgatataag gaggtcaaaa
      301 gagggtgtgg attttcttta cataaaggca tcaggtagtc atcctgcaag aaaggtccca
      361 ttaaataaat ctgctaaaaa ggcaattgat gcatacttag aaataaggcc tgaaacagaa
      421 gatgacactt tgtatgtaac aaagaatgga aggcccctgc ttgtaaggaa tataagaaca
      481 gctatagata aagcatttga gaaagcaagt attaaaaatg ctaaggtaaa cgatcttaga
      541 aatacattta tagcacatca tttagcaaag ggtgtaagtc ttgttactgt atcaaaatta
      601 gtaggtcata aaagaatatc taccacagag aagtacttag gacttgttac agataatagg
      661 ccagaggcag agaaattact caaagagttg taataatcag tattcattta aaaatgctaa
      721 actttctact tacttttcaa taaattcatc aaaggtagaa ctaaggctct cctcaatcaa
      781 attaaaccag acataatctt catatttagg acaaggattc tcttttatta attcatttcc
      841 tactttctga taaacagacc actctgattc tgaaatattg ctcttactaa gtttagaata
      901 gggaatttta tatttatatt cctccatctt acatacaaga ggagaaattc tctcaagtat
      961 aacaaaatct gccaaattcg ttgctttcca aaaaaagata aagataatat ttaaaagaat
     1021 aacccaaaca acaaaaatta ccattaaaac cctctgctta tccataaaac ttgtaaacaa
     1081 atttagatgg tgggagcaga tggaatcgaa ccatcgacca cggctttata agaaccgcgc
     1141 tctgccaact gagctatact ccctaaaaca gaatgatttt accaaaaaga agtaaattat
     1201 gcactactaa aaatcaatag gaggaaatcc tgatggatta ttttgtgcag attcattctc
     1261 tactctatct aaatctgctc ttctgtttat ttccatatta accaagttta tatccctacc
     1321 atattttaat ctagacattt tagcaataat atctttaacc tcaggattaa cttttagatc
     1381 aaaacctgtt tcatttacat atttaggacc atatctagca tcaagggaaa atgggctagg
     1441 atatgaattg tttgttagca tctttacata gtaatgtgca agcccctgat ttataatatc
     1501 cgtagcctta acatatgtct caaattgagg ttcaagaaac tgagcatctt cattactaca
     1561 tcttgtaata aagagagaac ctacattccc aaataccgca tttttaatat cttctggaag
     1621 ctgaccaata tattgatgtg ccagagtaag atttaatctg tactttctag cctcagacaa
     1681 tattgaagca aattcctgtg ttgcaaagtt ctgaaattca tcagcataga agaagaagtc
     1741 ttttctctca ccttctggaa tatcttccct gcttaaagca gctgccaaaa gcttttgaac
     1801 aagtaagaga cctagaaaag acatattctc ttctccaaga gcacctttag aaagatttac
     1861 aattaaaatc ttgcctgtat ccataacttc tctaaagtca aaagaagact tagactgccc
     1921 tattatattt ctaatagcca aattcgtagt aaatttatca aatttactaa taaagtagcc
     1981 aagactttct gattttgtct ttgcatctgt tttagcaact tgatcagtcc aataccttct
     2041 tacaagctca tccttaacaa gaggaagcca tttttcataa acccatttct catcttgaag
     2101 cattctaaca acctctatca gtgtagagcc ctgtacactc attgcagtaa gcatagagtt
     2161 tcttactgcc tgttgcatca ttggaccaac atacccttga ttatgagggt caaagagacg
     2221 tataaggaga tctataaaac tgtttacaat ctgatgttta tgttgctcat tataaaattc
     2281 taagatgttt aaaccaaaag gtctttcaaa a
//
LOCUS       DAOSAB010000097         2295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_31465_length_2295_cov_2.953571, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000097 DAOSAB010000000
VERSION     DAOSAB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31465_length_2295_cov_2.953571"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PK432_02155"
     CDS             complement(<1..436)
                     /locus_tag="PK432_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59760.1"
                     /translation="MTYLIIGSDSESIKEEITRLISRLWNKKINKELLEVKSADIHTI
                     TTENINSIGIEEIKRLQKEMIFTPYSESVQVALIFNADKLTEEAQNSFLKTLEESNNN
                     TAYILTTSNEKNLLPTIISRSLKVYTRDINVQNNSKGMPNTLT"
     gene            605..>2295
                     /locus_tag="PK432_02160"
     CDS             605..>2295
                     /locus_tag="PK432_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOK59761.1"
                     /translation="MAQETLLSYLQRQGIISEADAVKVQVERSRSPKNEETIIREMKL
                     ADDEQIAKAKSAIFNIPFVDLMENDVPEGVIAEVPIDSLKRYRAVPFERGDDYVKIAM
                     LDPFDIQAIQALQRKYPPNTKVIVYITAENGLNYILDRRMGDLMSSEVTEALEDVEVP
                     VQEINEDPNLISSIDLKNAPVARIVNSILQYAVVSKSSDIHVEPMETRLRVRFRIHGV
                     MTERLSLPKALAPAIVSRIKIMSNLKIDEKRVPQDGRFQVKVKNSKIDIRVSVMPTIH
                     GEKVVMRLLESDTEGLTLEGTGLRGNAYKLFLVALSVTNGIILVTGPTGSGKTRTLAC
                     ALMKVNDPSVNVISLEDPVEIRIPGVTQIQINPDVGLTFATGLRSVLRQDPDIVMVGE
                     IRDQETAELAIQASLTGHLVLSTLHTNSAAAAIPRLLDMGMEPYLLASTIRLLVAQRL
                     PRRVCQRCMKAVPATPAEMEVLKEILSGIEGFDLFKYTTQVINAKKAKGEEGASALKA
                     PEIGPDGEPIIYLYKGSGCDRCGGSGYVGRIGIFEVLDVNEKISRMVMENVTAQD"
BASE COUNT          708 a          324 c          484 g          779 t
ORIGIN      
        1 ttgtcaaagt attaggcatc cccttagaat tattttgtac atttatatcc cttgtatata
       61 cttttagaga tcttgagatg atggtaggaa gaaggttttt ttcatttgat gttgtaagga
      121 tatatgcggt attgttattg ctttcttcta aggttttaag aaatgaattt tgtgcctctt
      181 ctgttaattt gtcagcatta aagattaaag caacttgtac actctctgag taaggagtaa
      241 aaatcatttc tttttggagt ctttttatct cctctattcc tatgctattt atgttttctg
      301 ttgtaattgt atgaatatcg gcagatttaa cttcaaggag ctctttattg atttttttgt
      361 tccaaagtct tgagattaat cttgtaattt cttcttttat actttcgcta tcacttccaa
      421 taattaggta tgtcatatat ctatatttta tcatatttta gagtaaaaag cttgtgttta
      481 gagtgcttaa actgcttgat tttacagtat tttatgagat tttagcttta attacattat
      541 aggtcgtgaa attactctct tctcttgcta ttattacata atattaattt tactgcctat
      601 atttgtggct caggaaacac tgctttcata tttacaaaga caggggataa ttagtgaagc
      661 tgatgctgtt aaggtacagg ttgagagaag tcgctcacct aaaaatgaag agacgataat
      721 aagggagatg aagctcgctg atgatgaaca gatagctaaa gcgaagtctg caatctttaa
      781 tatccctttt gttgatttaa tggaaaatga tgttccagag ggtgttattg ctgaagtacc
      841 aattgatagt ttaaagagat atagggctgt tccttttgaa aggggagatg attatgtaaa
      901 aatagcaatg cttgatcctt ttgatataca ggctattcag gcattacaaa gaaagtatcc
      961 acctaataca aaggtaattg tatatattac agcagagaat ggattgaact atatccttga
     1021 tagaaggatg ggagatctta tgagctctga ggttacagag gctttagaag atgttgaggt
     1081 acctgtacag gagattaatg aagatcctaa tttaattagt agtattgatt taaaaaatgc
     1141 tcccgttgca agaattgtta actcgatttt acagtatgct gttgtatcaa agtcatcaga
     1201 tattcatgta gagccaatgg aaacaagact aagagtgaga tttagaattc atggtgttat
     1261 gacagagaga ctttcccttc ctaaagcact tgcacctgct attgtctctc gtattaagat
     1321 tatgtctaat ttaaaaattg atgagaagag agttccacaa gacgggagat ttcaggttaa
     1381 agtgaagaat tctaagatag atattcgtgt ctctgttatg ccaactatcc atggagagaa
     1441 agtggttatg cgtttacttg aatctgatac agaagggcta actttagagg gtactggttt
     1501 aagaggaaac gcctacaagc tttttctggt tgcactatct gttacgaatg gaataatact
     1561 tgttactggt ccaacaggaa gtggtaaaac tagaacactt gcatgtgctc tgatgaaagt
     1621 taatgaccct tccgttaatg ttatctcttt ggaggatcct gttgaaatta gaatacctgg
     1681 agttacacag atacagatta atcctgatgt tggtcttact tttgctacag gtttgcgatc
     1741 agtattaaga caggaccctg atatagtgat ggtaggtgag attagagacc aagaaactgc
     1801 agagctagct atacaagcat cgcttacagg acatttagtt ttatctactt tgcatacaaa
     1861 cagtgctgct gcagcaatac caagactact ggatatggga atggagccat atcttttagc
     1921 gtctactata cgtcttttag ttgctcagcg tttaccaaga agagtatgtc aaaggtgtat
     1981 gaaagctgta cctgcaacac cagcagaaat ggaggtactt aaggaaatat tatcagggat
     2041 tgaaggtttt gatttgttta agtatacaac tcaagttatt aatgctaaaa aggcaaaggg
     2101 agaagaagga gcttctgcac tcaaggctcc tgaaataggg ccagatggag aaccaatcat
     2161 atatctttat aaaggaagtg gatgtgacag atgtggaggt tctggctatg ttggtcgtat
     2221 tggtattttt gaggtattgg atgttaatga aaaaattagt agaatggtta tggaaaatgt
     2281 tacagctcag gatat
//
LOCUS       DAOSAB010000098         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_31512_length_2292_cov_3.890478, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000098 DAOSAB010000000
VERSION     DAOSAB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31512_length_2292_cov_3.890478"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..381)
                     /locus_tag="PK432_02165"
     CDS             complement(73..381)
                     /locus_tag="PK432_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59762.1"
                     /translation="MAQQNNAITAFLGGPSTLRPFFSNLMDEEFHLMGPEDLRFHLSW
                     DWMMPVWKKLRKELMATQADGSMLFALSKALDDVDIAAFHRISATYCVEWCKRKNIKL
                     "
     gene            complement(365..691)
                     /locus_tag="PK432_02170"
     CDS             complement(365..691)
                     /locus_tag="PK432_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59763.1"
                     /translation="MSIKKTKKQSKLTNKPLNEVIIKQVGFHKKWLDDKSGYWWEKTL
                     QSRIIPVNIVYDQDTNELILRIKILNDFKPYRREKSWDFIQIVPGTILNLKRILVNGA
                     LIGATK"
     gene            complement(651..971)
                     /locus_tag="PK432_02175"
     CDS             complement(651..971)
                     /locus_tag="PK432_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59764.1"
                     /translation="MEIPFKALCINDADRPSDIPLSQWIQKGKLYTVIKVGKMLVQGG
                     KIGFHLAEVNLDGCAPYLTYAANRFAVILNNKVIAELELNELLKEAKEEYEHKENKET
                     VKAD"
     gene            complement(955..1287)
                     /locus_tag="PK432_02180"
     CDS             complement(955..1287)
                     /locus_tag="PK432_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59765.1"
                     /translation="MAVTALKIGDQRLKFMTLPAELIKSKDGETEKLFLTVDLRICDE
                     DFIPMGVGSISVHEGDEESYHKALRAAAKEKGHFVPEESTDHQWHPDYNPVQDIDHEE
                     IKEDGDTV"
     gene            complement(1289..1534)
                     /locus_tag="PK432_02185"
     CDS             complement(1289..1534)
                     /locus_tag="PK432_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59766.1"
                     /translation="MTKQEEYSLMQNSKPYDLVTPVKNFKSIKWLKIWDYKNTPMLTT
                     FKTLTPPQHKHKLITAEEIDLRPMANYTIYKVTIKKN"
     gene            complement(1553..1852)
                     /locus_tag="PK432_02190"
     CDS             complement(1553..1852)
                     /locus_tag="PK432_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59767.1"
                     /translation="MIKVNDMWVCAHCHNVFNGDDEKEEAGYCSACAKKHENVHTNSQ
                     SKNWIKYIKELRDREWNYRQQTCSMHNFQKIPKSGMYLGTPLWRCIYCEAVVQAS"
     gene            complement(1849..2046)
                     /locus_tag="PK432_02195"
     CDS             complement(1849..2046)
                     /locus_tag="PK432_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59768.1"
                     /translation="MNTPLRSSRLIRRVILDENLPAGWPVKWGGKWDIYIDSKLVSTG
                     LRSLDWTGNQWKEFILKNVKK"
     gene            complement(1997..>2292)
                     /locus_tag="PK432_02200"
     CDS             complement(1997..>2292)
                     /locus_tag="PK432_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59769.1"
                     /translation="LNSAERISEQALVLRWFREKYNLHSTITSISQESWQWHITKPGE
                     SLGKLYEEDFYTYEEAELECLKKLIEIVKNGKSLEHSHEYSIKEQSVDKKSHT"
BASE COUNT          628 a          448 c          339 g          877 t
ORIGIN      
        1 tgttgagttt tcttaaatct tgtagcaatg tgtaagttat gctcttcttt ggttagtggt
       61 ttttcttcca tattataatt tgatattttt tctcttgcac cattctacac agtaggtagc
      121 actaattcgg tgaaaagctg cgatatctac atcatcaaga gccttactta aggcgaataa
      181 catagatcca tcagcttgtg tagccattaa ttcttttctc aacttcttcc agactggcat
      241 catccagtcc caagataggt gaaatctaag gtcttcagga cccataaggt gaaattcttc
      301 atccattaaa ttactaaaaa aaggtctgag tgtggatggt ccgccaagaa atgcggttat
      361 cgcgttattt tgttgcgcca atgagagctc catttactaa tattctttta aggtttaata
      421 ttgtgccggg tacaatttgt ataaaatccc agcttttttc tcttctataa ggtttaaaat
      481 cattcaatat cttaattcta agaattaatt catttgtatc ttgatcatac acgatattta
      541 cagggattat cctgctttgt agagtcttct cccaccaata gccggattta tcatctagcc
      601 attttttgtg aaaacccacc tgtttaatga ttacctcgtt tagaggtttg ttagtcagct
      661 ttgactgttt ctttgttttc tttatgctca tattcttctt ttgcttcttt taataattca
      721 tttaattcaa gttctgctat tactttgttg ttgagaataa cagcaaatct gttagctgca
      781 taagttaaat aaggagcaca gccatcaaga ttcacttcag ctaaatgaaa tcctatttta
      841 ccgccttgta caagcatttt tcctacttta atcactgtat aaagtttacc tttctgtatc
      901 cactgactta gtggtatatc tgaaggtcta tctgcatcat tgatgcatag ggctttaaac
      961 ggtatctcca tcttctttta tttcttcatg atcaatatct tggacaggat tgtaatctgg
     1021 atgccattga tgatctgtag attcttcagg aacaaagtgt cctttttctt tagcagcagc
     1081 tcttaatgct ttgtgatagc tttcttcatc accttcgtga acacttattg atcctacacc
     1141 cataggtata aaatcttcat cacaaattct cagatctact gttaagaata acttctcagt
     1201 ttctccatct ttggatttaa ttaattctgc aggaagagtc ataaacttga gtctctgatc
     1261 tcctattttt agtgcggtta cagccatatt agtttttctt tattgttact ttataaattg
     1321 tgtaatttgc cataggccta agatcaatct cctctgcggt gataagcttg tgcttatgtt
     1381 gaggaggtgt taaagtttta aatgttgtaa gcatgggtgt atttttgtaa tcccatattt
     1441 ttaaccattt gatacttttg aaattcttta ccggagttac aagatcataa ggctttgaat
     1501 tctgcatcag ggaatattct tcttgttttg tcattaggta aaagtaataa atttaacttg
     1561 cttgtacaac tgcttcacaa taaatacatc tccataaagg tgtacctaaa tacataccag
     1621 attttggaat cttttgaaag ttatgcatag aacaagtctg ctgcctataa ttccattctc
     1681 tatcccttaa ttctttgata tactttatcc aattcttaga ctgactattt gtatgcacat
     1741 tctcatgctt cttagcacaa gcgctacagt atccagcttc ttccttttca tcatctccat
     1801 tgaatacatt gtgacaatga gcacataccc acatatcatt tactttgatc atttttttac
     1861 attctttaaa ataaattctt tccattggtt acctgtccaa tcaagacttc ttaatcctgt
     1921 agaaacaagt ttactatcta tgtatatgtc ccatttacca ccccacttta ctggccagcc
     1981 agcaggtaag ttctcatcaa gtatgactct tcttatcaac cgactgctcc ttaatggagt
     2041 attcatggga gtgttccaga ctttttccat tttttactat ttctattagt ttcttaagac
     2101 actctagttc tgcttcttca taagtataaa agtcttcttc atataattta cccaaagact
     2161 caccaggttt tgtaatatgc cattgccaag actcttggct tatagatgta atagtagaat
     2221 gtagattata cttctctcta aaccatctca aaaccaaagc ctgttctgaa attctttcag
     2281 cactatttaa tg
//
LOCUS       DAOSAB010000099         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_32424_length_2247_cov_2.107664, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000099 DAOSAB010000000
VERSION     DAOSAB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32424_length_2247_cov_2.107664"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PK432_02205"
     CDS             complement(<1..341)
                     /locus_tag="PK432_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59770.1"
                     /translation="MKKRLYVYILVLILSSVLLSGCTKKESEETDTKTDTSVTEQEET
                     EEEKPAEEKPVEEKEESPQDSSSLEGFGKEKVVLGQASDFEYTLDSIETKEGEGYYEF
                     TFKISSSQEGAV"
     gene            429..1721
                     /locus_tag="PK432_02210"
     CDS             429..1721
                     /locus_tag="PK432_02210"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOK59771.1"
                     /translation="MKKEISMTDTQLQSKKVNTTQQQEPVIQTPTTQNITTAPQQEEK
                     KDKPKKKKTGLIIGISALAILTGIFLFYYFGIYKAKPYKKPSEVINLNNGYILPNSVE
                     NTFDKVPIPAPLEPKTEESPINGKLFTKTEIKDLQKRRPIAVMINNHAEARPQSGLNS
                     ADVVFETLAESGITRFLAIFWSEAPKKVGPIRSARQYYLEWLSPFDPLFIYDGCADTD
                     NPKTDACGNIHSYKIKNVATAGAWRWNDGVRYAPHNEYSSLFTAWEYAEDKGWDGMPT
                     IESWKFKKDATLDNRGQKTKVKILFHERLRNNGAYDSTWTYDIKTNSYYEEIGGKKLI
                     DQETNTQVYAKNLIIQEVEYSPAYDDKGRIILTTIGEGKATYFIDGKITTGKWKKTSR
                     TDRTTYYDNSGNEIQFNRGRTWISVVPLSIGKFDIIEQ"
     gene            1782..>2247
                     /locus_tag="PK432_02215"
     CDS             1782..>2247
                     /locus_tag="PK432_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK59772.1"
                     /translation="MLKSLFVSKVRIKVLAKYMEDVNKSYHVRGLVRELGEEINAVRR
                     ELLSLKKAGLLESHKEGNKIMYSINKQCPILWELRSMFFKESDIGKLLYETFLPLSGV
                     KVVIVTETFLKNKYLDSTDIDLLFLGDMKIREVSSAVSSVEKELGRSIRYTVM"
BASE COUNT          869 a          379 c          372 g          627 t
ORIGIN      
        1 acagcaccct cctgtgaaga agagatttta aatgtaaatt cataataccc ctctccctct
       61 tttgtctcaa tactatcaag tgtatactca aaatcactcg cctgccctaa aacaaccttt
      121 tcttttccaa acccctctaa actactacta tcttgaggac tctcttcttt ttcttcaaca
      181 ggcttttctt ctgcaggctt ttcttcttct gtttcttctt gttcagttac agatgtatct
      241 gttttagtgt ctgtttcctc actctctttc tttgtacatc cactaagcag tacactactt
      301 aaaattaaaa ctaaaatata tacatacaaa cgctttttca tattaatgtc tacttaatta
      361 atatatattt atactatcac atttactaaa tgattaacat tcttcctctt acatgttaaa
      421 ttataaatat gaaaaaagag ataagtatga cagatacgca gctacaaagt aaaaaagtta
      481 acactactca acagcaagaa ccagtaatac aaactccaac tactcaaaat attactactg
      541 caccacagca ggaagaaaag aaagataaac ctaaaaagaa gaaaacaggt ttgataattg
      601 gtatatctgc cctagctata cttacaggaa tattcctctt ttactatttt ggaatataca
      661 aagcaaaacc atacaaaaaa ccatctgaag ttattaatct aaacaatgga tatattctcc
      721 ccaactccgt agaaaataca tttgataaag taccaatacc agctccctta gaacctaaaa
      781 cagaagaaag tccaataaat gggaaactgt ttacaaaaac tgaaataaaa gatttgcaaa
      841 aaagaagacc cattgctgta atgataaata accatgcaga agcaagacct caatcaggat
      901 taaacagtgc agatgttgta tttgaaacat tagcagaatc aggaataact agattcttag
      961 caatattttg gagtgaagca cctaaaaaag ttggaccgat aaggagtgca agacagtatt
     1021 acttagaatg gctttctcca tttgatcctc tatttatata tgacggctgt gcagatacag
     1081 acaaccctaa aacagatgca tgcggaaata tacattctta caaaattaaa aatgttgcaa
     1141 cagcaggagc atggagatgg aatgatggtg taagatatgc cccgcacaat gaatactctt
     1201 ctctctttac agcatgggag tatgcagaag ataaaggatg ggatggtatg cctactatcg
     1261 agagctggaa atttaagaaa gatgcaactc ttgataacag aggacaaaaa actaaagtaa
     1321 aaatactttt tcatgaaaga cttagaaata atggagcata tgacagtaca tggacatatg
     1381 atataaagac aaactcatat tacgaagaaa tagggggaaa gaaactaata gatcaagaaa
     1441 caaatacaca ggtatatgca aagaacttaa taattcagga ggtagaatat tctcctgcat
     1501 atgacgacaa aggaagaata atactaacta caattggaga aggaaaagca acttacttca
     1561 ttgatggaaa aattacaaca gggaaatgga aaaagacatc aagaacagat agaacaacat
     1621 attacgataa tagcggaaat gagatacaat ttaatagagg aagaacatgg atttctgtag
     1681 ttcctctttc aataggaaaa tttgatataa ttgaacaata gaatgcccca ggtctaagag
     1741 ctgcccctct tagcctattt aatatttaat aaggagggga tatgcttaag agtttgtttg
     1801 tatcaaaagt aagaattaaa gtacttgcta aatacatgga agatgtaaat aaatcatacc
     1861 atgtaagagg gcttgtaaga gagctaggtg aggaaataaa tgcagtaaga agagaacttc
     1921 ttagtttaaa gaaagctgga cttcttgaat ctcataaaga gggaaataaa ataatgtaca
     1981 gtataaataa acagtgccct attctctggg aactacgctc aatgtttttt aaagaatctg
     2041 acattggaaa acttctttat gagacattcc tacctttaag cggagtcaag gttgtaattg
     2101 taactgaaac attcctaaaa aataaatatc tggatagcac tgatatagat ctcctctttc
     2161 ttggcgacat gaagataaga gaagtatctt cagcagttag cagtgttgaa aaagagcttg
     2221 gcagatcaat aagatatact gtaatga
//
LOCUS       DAOSAB010000100         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_32441_length_2246_cov_3.081698, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000100 DAOSAB010000000
VERSION     DAOSAB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32441_length_2246_cov_3.081698"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PK432_02220"
     CDS             complement(<1..578)
                     /locus_tag="PK432_02220"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOK59773.1"
                     /translation="MSNTTILSVTKKNIKRNKWLTASTILVTTIVFTLTSVFVSISIL
                     LSKTIDYYEQRAQVIVFFKKGTPEEDIFKFKDQIDNPELIEEIDYISQEEALEIYRED
                     FADDPDLISTVTADSLPASLEIRAKNIDALITIIDNINKAREINPNVDDVMYFEDVVN
                     NIKTLSRIINIGGIILITTMIIVTIALIRVTIG"
     gene            complement(838..1491)
                     /locus_tag="PK432_02225"
                     /pseudo
     CDS             complement(838..1491)
                     /locus_tag="PK432_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005386830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1530..>2246)
                     /locus_tag="PK432_02230"
     CDS             complement(1530..>2246)
                     /locus_tag="PK432_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOK59774.1"
                     /translation="TMSIHAGQGGTEAEDWTEMLFRMYTMYIEKKGWRYNITNMIKGA
                     EAGIATVTFDVIGKYSYGILKREHGTHRLVRLSPFNAQNLRQTSFAAVEVIPILKDVE
                     KDIEIPEGDIEFKAVRASGPGGQKVNKTSSAVQITHIPTGITVTCSEERSQLQNRQKA
                     MEILKGKLWKIMEENRLKEISDIKGDYKLAGWGNQIRNYILHPYKLVKDLRTGVESSD
                     PQSVLDGNIEQFLEAQLRIQ"
BASE COUNT          591 a          400 c          318 g          937 t
ORIGIN      
        1 cctattgtta ccctaattaa agcaattgta actattatca ttgtagtaat taatattatt
       61 cctcctatgt taattatcct tgatagtgtc tttatgttat taacaacatc ttcaaagtac
      121 ataacatcat caacatttgg atttatctct cttgctttat taatattatc aattatggtt
      181 ataagggcgt caatattctt tgctcttatc tctaaacttg ctggaagtga atcagctgtt
      241 actgttgaga ttaagtcagg atcatctgca aaatcttctc tgtaaatttc taaagcctct
      301 tcttgtgata tataatctat ttcttctatt aactcaggat tatctatctg atctttaaat
      361 ttgaatatat cctcttctgg agtacctttc ttaaagaaaa caattacctg agctctttgt
      421 tcatagtaat caatagtttt tgaaagaaga attgaaatag aaacaaatac agatgtaagt
      481 gtaaatacta tggttgttac aagtattgta gaggcagtaa gccatttgtt tctttttata
      541 ttcttttttg ttactgacag tattgttgtg ttactcattc ctttttttat ttaaatattt
      601 cttaataaaa tttgcaagtt ctctgttttc tttcttgctt aaattcatat tttttaattc
      661 atctgtcgta atgtttaata aaatatctgg agtctttatt gcatattttt caagtttttc
      721 ttttaatgat ttgcttagtt tgtcaaattc aggtgatagc tctgtatctt tcttttcttt
      781 aacttcctct ttctgtgttg gtgctggctt ggggataata atctctttag ctcctgtatc
      841 agaatttagc tttccatctt ttatatttat aactctgtcc tttgctttgt ttataagatt
      901 gaggtcatga gagataacaa gtactgtagt tcctgtttta ttaatagtct ttaatatatc
      961 taaaatctgc atagcattgt ctatatcaag attgcctgtt ggttcatctg caataaacaa
     1021 atctggttta ttagctaatg ctcgtgcaat tgcacctctt tgctgttctc cgcctgatag
     1081 ctcataaggg aaaagatctc ttctatctct taaaccaact aggtctagga gataatctgt
     1141 tgtatctttt atttctttgc tacttttatc aagaatttct agggcaaatg cgatgttttc
     1201 ttctaaagtt tttgtattaa ggagtttaat atcttgaaac acaacaccta gtctttgtct
     1261 gtatgctggg agtaatcgtc tatttaatct tggaacttca atgtcattaa agaaaatagt
     1321 accagcagta ggaacctctt ctcttatgag tagtttcatt attgtagatt ttcctgcacc
     1381 agaaggtcct attataaaag taaactcacc tgggtctact ctaaatgaaa catcttgaag
     1441 tgctgttata ttttctttat atttctttgt tacagtatcg aatattatca tgagcagttt
     1501 taatgtatat gagactatac acctatatct tactgtattc ttaactgggc ttcaaggaac
     1561 tgttcaatat ttccatcaag aacactttgc ggatcactgc tttctacccc agttctaagg
     1621 tcttttacaa gtttgtatgg atgcaatata tagtttctaa tctgatttcc ccatcccgct
     1681 agtttataat ctcctttaat atcagatatc tcttttaatc tgttttcttc cattatcttc
     1741 cacagctttc ctttgagtat ttccatagct ttttgtctat tctgaagctg acttctttcc
     1801 tcagagcatg ttactgtaat tcctgttggt atatgcgtta tttgtacagc agatgaagtt
     1861 ttgtttactt tctggcctcc tggaccgctt gctcttactg ctttaaactc aatatctcct
     1921 tcgggaattt caatatcttt ctctacatct tttaatattg gaattacttc tactgctgca
     1981 aaagatgtct gccttagatt ttgagcatta aatggggaaa gtcttactaa tctatgtgtt
     2041 ccatgctctc ttttaagtat tccatatgaa tattttccaa ttacgtcaaa agttactgtt
     2101 gctattcctg cctctgctcc ttttatcata ttagttatat tgtatctcca tccttttttt
     2161 tcaatgtaca ttgtatacat tctaaaaagc atctctgtcc aatcctctgc ttctgtacct
     2221 ccctgtcctg catgtattga cattgt
//
LOCUS       DAOSAB010000101         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_32761_length_2228_cov_2.901979, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000101 DAOSAB010000000
VERSION     DAOSAB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32761_length_2228_cov_2.901979"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PK432_02235"
     CDS             <1..213
                     /locus_tag="PK432_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59775.1"
                     /translation="GREYARYETVMSALTGSLGIIGGTIASLGEAYFDKVIAGAGVLI
                     ILSSIWPIFIYNHKDRKSKVENEQRN"
     gene            197..979
                     /locus_tag="PK432_02240"
     CDS             197..979
                     /locus_tag="PK432_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOK59776.1"
                     /translation="MNREIKKLFKSSIITFVLFDMDRTLVDTHTYFQEEMTNSILHTV
                     SKIFPAKPLKKQLKITKEIVDIANKIYEQQRYPILVDTLSELALSQYIKEKKVKINKK
                     KVYKELKSSYKDFYLTSPQPFPYTIGAIHKIKSFNIPIGVYSHAQNEWTRIKVDRIRN
                     EYLKRYGEGIKIPFFTTDITDLKDKEGWIKAGKYHKINPKRTLVVGDSLTADIYAAID
                     AGYKYAVYLTHTNKYVEVEQRAHAKIFVTKNLSTVFNQHSFL"
     gene            complement(957..>2228)
                     /locus_tag="PK432_02245"
     CDS             complement(957..>2228)
                     /locus_tag="PK432_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK59777.1"
                     /translation="KRKHGSILLIDMGKRIEKRKPSEVMTGIGGAGTFSDGKLTLSPS
                     LSHEKALHLVSKQEYQDILNYVDSIFTKFGVDSPYYPKETDELRKLIEEAEVNDVNLV
                     VRRARHVGSDKLKNFVKNFQEYILSQGVGIMDSTVVDDILVSEGKVNGVVTKEGKKFF
                     ADKVLLAPGRVNARWLQILADKYGIKYVNDKVEVGVRVEFPSLIMRRQAEVLYEVVYK
                     IRTKTYQDIVRTFCSCPNGKVSVEDYGSYVCVNGYSNSDNNGKNSNFAFLCEVSLKEP
                     LENSIAYAKSIAELTSLLGGGKPLLQTLYDFRDGRRSTWSRLEKSFVDPSLKDVVPGD
                     IAMGFPHRIVQNIIEGLEILDRVMPGINSGSTLLYAPEVKFRSSSIQTDKILQTAVSG
                     LFVAGDASGASGSITGAAATGIIAARGMSVG"
BASE COUNT          809 a          414 c          329 g          676 t
ORIGIN      
        1 ggaagagaat atgctagata tgagacagtt atgagtgcac ttactggttc actaggaatt
       61 attggaggaa caattgcaag cttaggagaa gcttactttg acaaagtaat agcaggagca
      121 ggagtgctca ttattttaag cagtatctgg cccatattta tatacaacca taaagacaga
      181 aaaagcaaag tagaaaatga acagagaaat taaaaaactt tttaagagca gtattattac
      241 ctttgttctt tttgatatgg atagaacact agtggatact cacacatact tccaagaaga
      301 aatgaccaat tctatacttc atactgtaag taaaatattt cctgctaaac ctttaaagaa
      361 gcagttaaaa attactaaag aaattgttga tatagctaat aaaatatatg agcaacaaag
      421 atatcctata cttgttgata cactctctga gttagcatta tctcaatata ttaaagaaaa
      481 gaaagtaaaa attaataaaa agaaggtgta taaagaatta aaatcttcat acaaagattt
      541 ctatttaacc tctccacagc catttccata tacaatagga gctatacaca aaattaaaag
      601 ctttaatata ccaataggtg tatactctca tgcacaaaat gagtggacaa gaataaaagt
      661 agatagaatt agaaatgagt atttaaaaag atatggggaa ggtataaaaa tacctttctt
      721 tacaactgat attacagatt taaaagataa agaaggatgg ataaaagcag gaaagtatca
      781 caaaattaac cctaaaagaa cccttgtagt aggagatagt cttacagctg atatttatgc
      841 agctattgat gcaggttata aatatgccgt atatctaaca catacaaata agtatgtaga
      901 agtagagcag agagctcatg caaaaatatt cgttactaaa aacttgtcaa ctgtatttaa
      961 ccaacactca ttcctctagc agcaataata cctgttgcag cagctccagt aattgaacct
     1021 gatgcaccag aggcatctcc tgcaacaaat aatccactaa cagctgtctg cagtatttta
     1081 tctgtttgta tgctagaaga tctaaattta acctcaggag cataaagaag agtagaacca
     1141 gaattaattc caggcattac cctgtcaagt atttcaagtc cttcaattat attttgaaca
     1201 attctatgag gaaaccccat tgcaatatca ccaggaacaa catcttttag agaagggtca
     1261 acaaaagatt tctcaagcct tgaccatgta cttcttcttc catctctaaa atcatacaaa
     1321 gtttgaagca aaggttttcc tcctccaagc aaagaagtaa gctctgctat tgattttgca
     1381 tatgctattg aattttcaag aggttctttt aaacttactt cacaaagaaa agcaaagttc
     1441 gaatttttac cattattatc agaattagaa tatccattaa cacaaacata gctaccataa
     1501 tcttctacag aaactttacc gttaggacag ctacaaaatg ttcttacgat gtcttgatat
     1561 gtcttagttc taattttata aaccacttca tacaagactt ctgcctgtct tcgcatgata
     1621 agggaaggaa attctactct tacaccaacc tctaccttat cattaacata tttaattcca
     1681 tatttatcag ctaatatctg aagccatcta gcattaaccc tgccaggagc aagcagtact
     1741 ttatcagcaa aaaatttctt tccttcttta gtaacaactc cattaacttt accctcagaa
     1801 acaaggatat cgtctactac tgtactatcc attatcccaa caccttggga gagaatatat
     1861 tcttggaaat ttttaacaaa gttttttaac ttatcagatc ctacatgtct tgcccttcgt
     1921 acaacaagat taacatcatt tacttctgct tcttcaataa gctttctaag ctcatctgtt
     1981 tctttaggat agtatggaga gtcaacacca aacttagtga atatactatc aacataattt
     2041 aatatgtctt gatactcctg ctttgagact aaatgaagag ccttctcatg agaaagacta
     2101 ggagaaagag taagttttcc atctgaaaat gtccctgcac ctccaatccc tgtcattacc
     2161 tcactgggct ttcgcttctc aattcgctta cccatgtcaa taaggagaat actaccatgc
     2221 tttctttt
//
LOCUS       DAOSAB010000102         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_33360_length_2197_cov_4.410364, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000102 DAOSAB010000000
VERSION     DAOSAB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33360_length_2197_cov_4.410364"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1844)
                     /locus_tag="PK432_02250"
     CDS             complement(27..1844)
                     /locus_tag="PK432_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59778.1"
                     /translation="MELRLTKKNSIIFAVIIVILCGSLGYLVWRVNQPETTAPTESEA
                     AQPCDPPCGNKPDLANWARKPYNQSMKNISDSSKEDQAKGYSIPSKITIPAGVSGEIV
                     LYYKSIDGESGSLASFELNGQAFNVKSLDENRRAIINTGLHVKGGETFTVNSQILNGS
                     GEFAPGCSGSNWNRYWSLGWIAPSNGTCGTTFGGPPQGGICTPFVPANVSADISWATS
                     SGNKIVSQQCWADWMEWVGDYDFNDYLLIVTVKSDASCGDGVVGNTEGEECDPPNDPC
                     TKDGHSGKCSSTCKCVPDPYCGDGVVGTGEECDPPNNSCTKDGHSGKCSSTCKCVLDP
                     YCGDGVVGTGEECDPPGDSCTKDGNTGACSNTCMCVVSTYCGDGKLKDDEECEVGDPA
                     GVKCTWTSCNQSACVCLPPGLSISKTVAKQCTGEGSENPNSELLYTITVSNTGTGVGQ
                     ISKIEDTLDTKIVTAGIVPTDITEGGSYLGGKITWLFSSPLTISPGETKVYSYKMMID
                     KSNFGIYANTVVLTPVSGDPIQATATINADCIITAPIVPQTGIFDSTVGRIAGGLGLI
                     VLGGFVYSMPSRVFLLNRESDERRRNKYRVKFESKIFKK"
     gene            complement(1884..>2197)
                     /locus_tag="PK432_02255"
     CDS             complement(1884..>2197)
                     /locus_tag="PK432_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOK59779.1"
                     /translation="NAVSGPVGIYFIVDYFKTLGLISFLGVLADLSLSLALINIFPIP
                     ALDGGRFFILLIESIVRKDLDERVEAAIINISFIFLILFIILIMVKDIININELKSLF
                     K"
BASE COUNT          662 a          512 c          294 g          729 t
ORIGIN      
        1 attttacaag acactattta tgaaacttac tttttaaaaa ttttactctc aaattttacc
       61 ctgtatttat tccttcttct ctcatctgat tccctgttaa gcagaaatac tctacttggc
      121 atactatata caaaaccacc taaaactatt aatcctaacc ctcctgctat cctacctact
      181 gtactatcaa atatccctgt ttgaggtact attggtgctg ttattataca gtctgcattt
      241 attgttgctg ttgcttgtat agggtctcca cttacaggtg ttaatactac agtattggca
      301 tatataccaa aattgctctt atctatcatc atcttgtatg aatacacttt tgtttctcca
      361 ggagaaatag ttaatggtga actaaataac catgtaatct tccctcctaa gtaactacct
      421 ccctctgtaa tgtctgttgg aactattcct gctgtaacaa tctttgtatc aagggtatct
      481 tctatctttg atatctggcc tactcctgta cctgtattac ttactgttat tgtatacaat
      541 aattcagagt tagggttctc actaccttca cctgtacatt gcttagccac tgtttttgat
      601 atactcaacc ctggtggtaa acatacacaa gcactctgat tacatgatgt ccatgtacac
      661 tttactcctg ctggatcacc aacctcacat tcttcgtcat cttttaattt cccatctccg
      721 caatatgtac taactacaca catacatgta tttgaacagg ctcctgtatt tccatctttt
      781 gtacaagagt caccaggtgg atcacactcc tctcctgttc ccactactcc atctccgcag
      841 taaggatcta gtacacattt acatgtacta gaacatttcc cactatgacc atcttttgtg
      901 catgaattat taggtggatc acactcttct cctgttccta ctacgccatc cccacagtaa
      961 ggatcaggta cacacttaca tgtactagaa catttcccac tatgaccatc ttttgtacaa
     1021 ggatcattag gtggatcaca ctcttctcct tctgtatttc ctactacacc atctccgcag
     1081 gaagcatctg atttaacagt aacaattaaa agatagtcat taaaatcata atccccaacc
     1141 cactccatcc aatctgccca acactgttga cttacaattt tattaccaga gcttgtagcc
     1201 caagatatat ctgcagaaac atttgcaggt acaaaaggtg tacatattcc cccttgagga
     1261 gggccaccaa aagtagtacc acaagtcccg ttactaggag caatccaccc taatgaccaa
     1321 tacctattcc aattagagcc tgaacaacct ggtgcaaatt caccactacc atttaagatt
     1381 tgagaattta cagtaaatgt ttctccaccc tttacatgta gacctgtatt tataattgcc
     1441 ctcctgtttt catccaagct tttaacatta aaagcttgtc cattaagctc aaatgatgca
     1501 agactcccac tttcgccatc tatactcttg taatacaaaa ctatttctcc acttacacca
     1561 gcaggtattg ttatctttga gggaatagaa taaccttttg cctgatcctc cttggatgaa
     1621 tcagaaatgt ttttcatact ttggttgtat ggttttctag cccaatttgc taaatcaggt
     1681 ttattaccac aaggagggtc acaaggttga gccgcttcac tttctgttgg tgctgttgtc
     1741 tcaggctgat ttactctcca tactaaatat cccaatgacc cacataatat aactattatg
     1801 actgcaaata taatcgaatt cttttttgtt aaccttaatt ccatacgagc agttaattta
     1861 taatttctta ctctaacact acactactta aacaaacttt tcaactcatt aatattaata
     1921 atatctttaa ccataattaa gataataaag aggattaaaa agataaagct tatgttgata
     1981 atagcagcct ctaccctttc atctaaatcc ttccttacta tcgattcaat tagaagtata
     2041 aagaatctgc cgccatcaag tgcaggaata ggaaaaatat ttatgagagc gagtgataga
     2101 gataagtctg caagtacccc aaggaatgaa attaaaccta aagttttaaa ataatcaact
     2161 ataaagtaaa ttccaacagg tccactcaca gcatttg
//
LOCUS       DAOSAB010000103         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_33723_length_2181_cov_3.103481, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000103 DAOSAB010000000
VERSION     DAOSAB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33723_length_2181_cov_3.103481"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /gene="gatC"
                     /locus_tag="PK432_02260"
     CDS             complement(<1..182)
                     /gene="gatC"
                     /locus_tag="PK432_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOK59780.1"
                     /translation="MDKRTLRHIAKLSRISLTDEELEKFTPQMQTILDNAKILQEVNT
                     NNITPMKRHIPFSQLR"
     gene            complement(175..1686)
                     /gene="gatB"
                     /locus_tag="PK432_02265"
     CDS             complement(175..1686)
                     /gene="gatB"
                     /locus_tag="PK432_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOK59781.1"
                     /translation="MENSKYDVIIGLEIHVQSNTKSKMFCSCPTHYFEQEPNTHVCPT
                     CLGLPGALPVPNKRAIELCILLGLALHCDIDDEIHFDRKHYFYPDLPKGYQISQYKRP
                     ICSDGYLEIKDSDGKKYSVGIERIHQEEDVAKLLHYDKNGIAYSLIDYNKSGVPLIEI
                     VTKPEIRSAYQARKFAEMIRQIVRYLNISDADMEKGQMRCEPNISVQEKGKWKYSNGR
                     ILPIGNYKLNPKTEIKNIGSISAVEKSIEYEVKRMIDELEKGKTLIQQTRGWNADKGI
                     TEFQRSKETADDYRYMPEPDIPIIDISIDDVKRVSNELVELPDEKEERYKKEYKLSDY
                     DSSVLVADRDISVYFEELVALLSSSMPVQKAAKSASNWITGTVFAISNDKKIEINEKN
                     IPKSSLALLITEFEKKTLTRNKAESLLRESIENNKDLKELIAKATQEKTVTLGNIDSI
                     VDEIINSNPKAVNDYKSGKEATIGFLIGQVMQKTKGSADPNKVKSILIKKLNG"
     gene            complement(1725..>2181)
                     /locus_tag="PK432_02270"
     CDS             complement(1725..>2181)
                     /locus_tag="PK432_02270"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOK59782.1"
                     /translation="LNPHNIIPISKAFNDISEDIESAFKLAKSKRDLTIFIEAILYTL
                     SLTVIDTSKRVTQEYDKRLNIRRNLKDDLNSRQIKLMEYLSDNPKITRREFSKMMGVS
                     FMTAYRDLQDLLNKKYISQKGEGRGSYYFIPDEESKEQERVENEIKIFE"
BASE COUNT          612 a          376 c          302 g          891 t
ORIGIN      
        1 tctcttaact gtgagaaagg gatatgcctt ttcataggag taatattatt tgtattaacc
       61 tcttgtagta ttttggcatt atcaagaata gtttgcatct gaggagtaaa cttttctaac
      121 tcctcatctg taagagatat tctggatagc tttgcaatat gtcttaatgt ccttttatcc
      181 atttaacttt tttattaaaa ttgattttac tttatttgga tctgcactcc cttttgtttt
      241 ttgcataact tgacctatta aaaacccaat tgttgcttct ttcccagatt tataatcatt
      301 aacagcttta ggattagaat ttataatctc atctacaatt gagtcaatat ttcctaatgt
      361 aactgttttt tcctgtgttg cttttgctat taactctttt aagtctttat tattctcaat
      421 agattctcta agtaaactct ctgctttgtt tcttgttaga gttttctttt caaattctgt
      481 aattagaagt gcaagagagc ttttggggat atttttctca tttatttcta tctttttatc
      541 atttgatatt gcaaatacag tacctgttat ccaattagat gcagacttag cagccttttg
      601 tacaggcatt gaagatgaga gaagtgcaac aagctcttca aaatagacag aaatatctct
      661 gtcagcaact aacacagagc tatcataatc agagagtttg tactcctttt tgtatctctc
      721 ttctttttca tcagggagtt caacaagctc atttgatact ctttttacat catcaataga
      781 gatatcaata attggaatat ctggttcagg catatatctg taatcatcag ctgtttcttt
      841 tgacctttga aactctgtta tacctttgtc tgcattccag cctcttgtct gctgaattaa
      901 agtttttcct ttttcaagtt catctatcat tctttttact tcatactcta ttgatttttc
      961 aacagctgaa atagaaccga tattctttat ttcagtttta ggatttaact tgtagttacc
     1021 aattggtaaa attctgccat ttgagtattt ccattttcct ttctcttgta ctgatatatt
     1081 aggttcacat ctcatttgtc ctttttccat atctgcatca gagatattta gatatctaac
     1141 aatctgtctt atcatttcag caaactttct agcttggtat gcacttctta tctctggttt
     1201 cgttactatc tcaatcaaag gaactcctga tttgttgtag tcgataaggg agtaggctat
     1261 accattcttg tcataatgga gtaattttgc aacatcttct tcttggtgaa ttctctcaat
     1321 accaacagaa tattttttcc catcactatc ttttatttct agatatccat ctgagcatat
     1381 cggcctttta tattggctaa tctgatatcc tttaggaagg tcagggtaaa aatagtgttt
     1441 tctatcaaaa tgtatttcat catctatatc acagtgcagt gcaagaccaa gaagtataca
     1501 aagttcaatt gctcttttat tgggtacagg taaagctcct ggtaaaccta aacatgtagg
     1561 gcacacatga gtatttggtt cttgttcaaa gtagtgagta ggacatgaac aaaacatttt
     1621 acttttagta ttgctttgaa catgtatttc taatccaatt attacatcat attttgaatt
     1681 ttccatttat agtgatgata tcaataatat atatctatat caatttactc aaatatttta
     1741 atttcatttt caaccctttc ctgctcttta ctttcttcat cagggataaa atagtaagaa
     1801 cctcttcctt ctcctttctg agatatgtac tttttattaa gtaaatcttg caggtctctg
     1861 tatgcagtca taaaggatac tcccatcatt ttactgaact ctcttctggt aattttagga
     1921 ttatcactga gatactccat taatttaatc tgtctagaat ttaaatcatc ttttaagttt
     1981 ctccttatat ttaacctttt atcatactct tgtgttactc tttttgatgt atcaattaca
     2041 gtaagggaga gagtataaag gatggcttct ataaatatgg ttaaatctct tttactttta
     2101 gctaatttaa aagcagattc tatatcctca ctaatatcat taaatgcttt tgatattgga
     2161 ataatattat gtggattaag g
//
LOCUS       DAOSAB010000104         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_34909_length_2128_cov_3.503618, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000104 DAOSAB010000000
VERSION     DAOSAB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34909_length_2128_cov_3.503618"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1800)
                     /locus_tag="PK432_02275"
     CDS             complement(94..1800)
                     /locus_tag="PK432_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOK59783.1"
                     /translation="MEERISVKFGGQSGQGINLLGQLISRALKESNYHVFGYREYPSI
                     IKGGFASYQIDFGSSEILSPEKKCDILACIAQEALEEYLFTVSSNGVIFHSVKDFEFS
                     KKAKEYINKNSIKVIYIDLRKMAQEQKAPAIMENMIMLGSIWKVLGQDIKTLTDSVVK
                     YFSKKEGIDIEAEKRCLNAGYSLEEIKDDEIKDVPKSIDKNWEKSLILTGNQAVALGA
                     ISAGCRAYYAYPMTPSTPILELLGDTYMKTGILVKQAESEITAVQMVLGSMYMGARAF
                     TATSGGGFDLMTESISMSAMTEIPLVIVLGQRAGASTGLPTWTGASDIDVAVHSGHGE
                     FPRCVLAASDPIESYELIQEAFNIAEVYQLPVILLTEKHVAESMFNISNLPKAKKIKR
                     GLSQNGTKRYLVTKSGISPRWLPMPGRKTYLTNSDEHDEYGVSSESDIDTKIMTEKRM
                     KKVETLSKTIPAPKYFGDKDPTIVFVGMGSVKNAVLDAMKISRRRIGYLHYKYIYPLK
                     YEKILQFAKDGVKIVLIENNFNNGFGRLIKEESNFYITNTLKKYNGRPFFVDDILEYL
                     KQ"
     gene            1902..>2128
                     /locus_tag="PK432_02280"
     CDS             1902..>2128
                     /locus_tag="PK432_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59784.1"
                     /translation="MIDKCQITVQSGKGGDGIIAFDKFRKADGGDGGKGGDVYIVGDT
                     NVYDLGKISQIKKFKANDGERGEKNKRKGKDG"
BASE COUNT          649 a          387 c          323 g          769 t
ORIGIN      
        1 aagctttttt aagtgccatg tgaattgcaa agtttccaca tccagggcac catgtatttt
       61 taatatctgt attgtaatct tgtaaattta ccttcattgt tttaaatatt ctaaaatatc
      121 atctacaaaa aatggtctac cattatattt ctttaatgta tttgtaatgt agaagttgga
      181 ttcttctttt atcaatcttc caaaaccatt attaaagtta ttttcgatca gtactatttt
      241 taccccatct tttgcaaact gaagaatttt ttcatattta agagggtata tatatttata
      301 atgaagatac cctattcttc tcctgctaat tttcatagca tctaatactg cattcttaac
      361 acttcccatt ccaacaaata ctattgtagg atctttatca ccaaagtact taggagcagg
      421 aatggtttta gatagtgttt ctactttttt cattcttttt tctgtcataa tctttgtatc
      481 aatatcactt tcactgctta ctccatactc atcgtgctca tcgctgtttg ttaagtatgt
      541 ttttctccca ggcattggta accatcgtgg agatatgcca cttttagtta ctaaatatct
      601 tttagttcca ttttgtgaga gtcctctttt tatcttttta gctttaggta ggtttgagat
      661 attaaacata gattctgcaa catgtttttc tgtaagaagt attacaggaa gttgatatac
      721 ttctgcaata ttaaatgcct cttgaattag ctcataactc tcaattgggt cgcttgctgc
      781 aagtacacat cttggaaact ctccatgacc tgaatgaaca gccacatcta tatcagatgc
      841 acctgtccat gtaggaaggc ctgttgaagc ccctgcacgc tgtcccagaa caatcacaag
      901 agggatttct gtcattgcac tcatagaaat gctctctgtc attaaatcaa agcctcctcc
      961 cgaggttgct gtaaatgctc ttgcacccat atacatactt ccaagtacca tttgtacagc
     1021 agttatttca ctttctgcct gtttaacaag aatacctgtt ttcatatatg tatcaccaag
     1081 taattctaaa atgggtgtag aaggagtcat agggtatgca taatatgctc tacatcctgc
     1141 actaatcgca ccaagtgcga cagcttgatt tcctgtaaga attaatgatt tctcccaatt
     1201 cttgtcaata cttttaggta catcctttat ttcatcatct ttaatttctt ctaaagagta
     1261 tcctgcgttt aagcatctct tttcagcctc aatgtctatt ccttcttttt ttgaaaagta
     1321 tttaactaca gaatcagtta gtgttttaat gtcctgtcct aaaaccttcc atatacttcc
     1381 gagcattatc atgttttcca ttattgcagg agctttctgc tcttgggcca tctttcttaa
     1441 gtcaatatat ataactttaa tactgttttt gttaatatat tcttttgcct ttttactaaa
     1501 ttcaaaatct tttactgaat gaaaaattac accattgctg ctaactgtga aaagatattc
     1561 ttctaatgcc tcttgtgcaa tacatgctaa tatatcacac ttcttttcag gtgaaagtat
     1621 ttcagatgat ccaaagtcaa tctgatacga tgcaaatcca cctttgataa ttgaaggata
     1681 ttctctatac ccaaatacat gataatttga ctctttcagt gccctactta ttaactgacc
     1741 taacaagtta ataccttgac ccgattgtcc tccaaattta acagaaattc tctcttccat
     1801 ctgtaattat ctattcataa atatctcttt tagagtatca ttcaagaaaa atattgtcaa
     1861 agaaagttac aatataggat aaagtaaggt aaaataagca tatgattgat aaatgtcaaa
     1921 ttacagtaca atcaggtaaa ggtggtgatg gaattatcgc ttttgataaa tttaggaagg
     1981 cagatggagg tgatggagga aaaggaggag atgtatatat agtaggtgat acaaatgtat
     2041 atgaccttgg taagatatct cagattaaga agtttaaagc aaatgatggt gaaaggggag
     2101 aaaaaaacaa aagaaaaggg aaggatgg
//
LOCUS       DAOSAB010000105         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_34984_length_2125_cov_2.945411, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000105 DAOSAB010000000
VERSION     DAOSAB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34984_length_2125_cov_2.945411"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PK432_02285"
     CDS             complement(<1..435)
                     /locus_tag="PK432_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOK59785.1"
                     /translation="MRIKYLINKDSRVKDFNILIKNNFPNLLTEKKPELYLVAGGDGA
                     MLHAIHKTIGKGIPYLGKAMGTFNFLMNQFDDDIQTINKILNDEIKIDTLSSHAIQVY
                     LNRKKLGEAVNDVILGDKITSYFTFNISTEQKDFDNFEIKGGG"
     gene            595..879
                     /locus_tag="PK432_02290"
     CDS             595..879
                     /locus_tag="PK432_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010302349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOK59786.1"
                     /translation="MQNKLFVGNLSWDTTDETLSQLFSTVGEVTEAKVIVDKFKGRSK
                     GFGFVTMASEELAQKAMEELNGKELDGRAINVSIARPQREEGNRRENFYR"
     gene            complement(916..>2125)
                     /locus_tag="PK432_02295"
     CDS             complement(916..>2125)
                     /locus_tag="PK432_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOK59787.1"
                     /translation="DTMARAVTNIEKDLEQRTAFLRNIGKEVEAQRLEQRTRYDIEMM
                     TQIGFCKGMENYSIYFDGRKTGQPPFTLLDYFPDDYLLFIDESHITVPQIGGMYNGDR
                     ARKTNLVEHGFRLPSAYDNRPLNFNEFRKKQGTTIYFSATPDEWEIRDSRNTVVELLT
                     RPTGLLDPKIEVRKTKNQIDDVISEVRKNVERKERVLITTLTKRMAEDLTSYLADFDI
                     KVAYLHSDIDTVERVEILRNLRLGEFDVLVGINLLREGIDLPEVSLVIILDADKEGFL
                     RSKISLVQTIGRAARHVNGRVIMYADTITESMKYAIDETNRRRKYQEEYNRIHNITPT
                     SIKKEIRDSLVQNYDNEKRDLDIEIEKLSEKQKKMLIKDLRAKMLLAADNLQFEKAAE
                     LRDKIKELSI"
BASE COUNT          634 a          380 c          303 g          808 t
ORIGIN      
        1 accacctcct tttatttcaa agttatcaaa atctttctgt tctgttgaaa tattaaaagt
       61 aaaatagctg gtaattttgt cgcctagaat aacatcattt acagcctctc caagcttctt
      121 tctatttaaa tatacctgta tagcatgtga agaaagggta tctattttta tctcatcatt
      181 taatatttta tttattgttt gtatatcatc atcaaactga ttcataagga agttaaaagt
      241 acccatagct ttacctagat atggaattcc tttaccaatt gttttatgaa ttgcatgaag
      301 catagcacca tcaccccctg caacaagata tagctcaggt ttcttttctg taagaaggtt
      361 tggaaaatta ttctttatta atatattaaa atcctttact ctagaatctt tatttattaa
      421 gtactttatt ctcatgaaat agagtatatc aaattgaggt cgtattgtat ttatattaaa
      481 tatggtataa ttaggtgtct atgttaagaa aggtgttaat gcattaacac cccgtgtagg
      541 ttaccttaaa aagtgtgctt atccgtagtc tgtttatttt ttatttataa ttaaatgcaa
      601 aacaaacttt ttgttgggaa cctgtcatgg gataccacag atgagacttt gtctcagctt
      661 ttctccactg ttggagaagt aacagaagca aaagtaattg ttgacaaatt caaaggaaga
      721 tcaaaaggct tcggatttgt tacaatggct tctgaagagt tggctcagaa agcaatggaa
      781 gagcttaacg gaaaagagct tgatggcaga gctatcaatg tctctattgc aagacctcag
      841 agagaggaag gcaatagaag agagaatttc tatagataat tattaatagt tatttggaaa
      901 ttaaaaagag ctgggctaaa tactcagctc ttttatttta tcccttaact ctgctgcttt
      961 ttcaaactgt agattatctg ctgcaagaag catttttgct cttaaatctt taattagcat
     1021 tttcttttgt ttttcactta gcttctctat ttcaatatct aaatctcttt tctcattatc
     1081 atagttttga acaagtgaat cccttatctc cttttttata cttgttggag taatattgtg
     1141 tattctattg tattcttctt gatattttct tcttctattt gtttcatcta ttgcatattt
     1201 catgctttca gtaattgtat ctgcatacat aattactcta ccgttaacat gtcttgcggc
     1261 tctccctatt gtctgaacta aactaatctt agatcttaaa aacccctcct tgtcagcatc
     1321 aagtattatt acaagagata cctctgggag atcaataccc tctcttaaca agtttattcc
     1381 aactaataca tcaaattctc caagtcttaa gtttcttaat atttcaactc tttcaacagt
     1441 atcaatatca gagtgaagat atgccacctt tatatcaaaa tctgccaagt atgaagttaa
     1501 atcttctgcc attcttttag ttaatgtagt aatcaatact ctttctttcc tttctacatt
     1561 ctttcttact tctgaaataa catcatcaat ctgattcttt gtttttctta cttctatctt
     1621 agggtcaaga agacctgtag gtcttgtaag aagttctaca acagtatttc tactatctct
     1681 aatctcccat tcatctggtg ttgcagaaaa gtatattgta gtaccttgtt tttttctaaa
     1741 ctcattaaaa ttaagaggtc tgttatcata tgcagaagga agtctgaacc catgctcaac
     1801 tagatttgtt tttcttgctc tgtccccgtt atacattcct ccaatttgtg gaacagtaat
     1861 atgagattca tcaataaaca gaaggtaatc gtctggaaag taatcaagta gagtaaaagg
     1921 aggctgtcct gtttttcttc catcaaaata tatagagtag ttttccattc ctttacaaaa
     1981 ccctatctgt gtcatcattt caatatcata tctagttctc tgttctaacc tttgagcctc
     2041 aacctcctta ccaatatttc tgagaaatgc agttctttgt tctaaatctt tctcaatatt
     2101 tgtaacagct cttgccattg tgtct
//
LOCUS       DAOSAB010000106         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_35576_length_2101_cov_3.258065, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000106 DAOSAB010000000
VERSION     DAOSAB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35576_length_2101_cov_3.258065"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PK432_02300"
     CDS             <1..1168
                     /locus_tag="PK432_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOK59788.1"
                     /translation="TYEEKDSLGNTILTAEYEPILPRSGKDFTLTIVPSIQNKVESIL
                     KTHVEKTGSRSGTAIIMEPKTGKIISMANYPTYNPNEYWRVSDPWIFKNLAVADVYEY
                     GSVQKPITISIALESNSIDKDYKCNDPTGYLDLYEVTKYADLKGRKIYTWNRKPGGNQ
                     TLADILKNSNNPCIARVALEIKPQYFYDKLKEFGIGSFLGIGLQEESNSYLKPFNEWT
                     RLDIITASYGQGISATPLQILSAISTIANNGVRMRPYIISKISNDEEEIMYEPQILSQ
                     PISEQTAQAVAEMMTHVIEDGGISPTYVKKLKDYYIAGKTGTAEVAKKEGGYEEDKTI
                     TTFIGFTPADDAKMIMLVRLEHPQTDELAAATVVPLWTDIFLSIANDLEIPKRN"
     gene            1210..>2101
                     /locus_tag="PK432_02305"
     CDS             1210..>2101
                     /locus_tag="PK432_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59789.1"
                     /translation="MTDILKESLQYLFISTAISMLFAPIMINILYRFNQVSEIKKTKL
                     ASEQGTNSVFMRIMRTAKTNGTPNMGGVLIWLIVPLVTYIFAPQFSQLNALLAGFLLF
                     GLWGFIDVAVFTNGLKNNEKFRALQETFEWRLIKLVIAIVLNIGIMYMLYKTGTFDTL
                     SLTNTIAISVTPAILILLAIIGQFAIYSGELTDGLDGLMVGIFIIITTAMFFLLTVQQ
                     KYEYLPFLAITLGVLIVDLYFNIPPARFWNGGPGAMPISFAMFFIGLTTGNIIPYFLM
                     SFITWIIMLSSAIQMISIKFF"
BASE COUNT          786 a          336 c          357 g          622 t
ORIGIN      
        1 tacatatgaa gaaaaagatt cgctaggaaa tacaatacta acagcagaat atgagcctat
       61 ccttcctaga tcaggaaaag attttacact tacaattgtc ccttcaatac aaaataaagt
      121 agaaagtata cttaaaactc atgttgagaa aacaggttct agaagtggta ctgcaataat
      181 aatggaacct aagacaggaa aaatcatttc aatggcaaat tatcccacat acaatcctaa
      241 tgagtactgg agagtatcag atccttggat ttttaaaaat ttagctgttg cagatgtata
      301 tgaatatgga tctgttcaaa aaccaattac aatttcaatt gcactagaat caaattctat
      361 tgataaagat tataaatgca atgatccaac aggatactta gatttatatg aagttactaa
      421 atatgcagac ttaaagggaa gaaagatata tacatggaat agaaaacccg gaggcaacca
      481 aactttagca gatattctaa agaactctaa taacccttgt atagcaagag ttgcactaga
      541 aataaaacct caatattttt atgacaaatt aaaagagttt ggaattggta gttttctagg
      601 cattggtctt caagaagaga gtaatagcta tttaaaacca tttaatgagt ggacaagact
      661 tgacattatt acagcatcat acgggcaggg tatttctgca acacctctac aaattctttc
      721 agcaataagt acaatagcaa acaatggtgt aagaatgaga ccgtatatta tctctaaaat
      781 ctcaaatgat gaagaagaaa taatgtatga acctcagata ctttcacagc cgatatcaga
      841 acaaacagca caagcagtag cagaaatgat gacacatgta attgaagatg gaggaataag
      901 tcctacatat gtaaaaaaat taaaagatta ctatattgca ggaaaaacag gtactgcaga
      961 agttgcaaaa aaagaaggag ggtatgaaga agataaaaca ataacaacat ttattggctt
     1021 tactcctgca gatgatgcaa agatgataat gcttgtgaga cttgaacatc cacaaacaga
     1081 tgagcttgca gcagcaacag ttgtaccatt atggacagat atattcttaa gtattgcaaa
     1141 tgacctggaa ataccaaaaa gaaattgaga taatactaag tacagtaatt taatttaaca
     1201 cttacagata tgacagacat acttaaagaa agcttgcagt accttttcat atcaactgca
     1261 atttctatgc tctttgcccc aataatgatt aatattctat acaggtttaa tcaggttagc
     1321 gagataaaaa agacaaagct tgcatctgag caagggacta attctgtctt catgagaata
     1381 atgagaacag caaaaactaa tggaactcct aatatgggag gtgtcctcat ctggttaatt
     1441 gtcccattag taacatatat atttgcacca caattttctc aactaaatgc acttttagct
     1501 ggttttctac tttttggcct ttggggattt attgatgtgg ctgtttttac aaatgggctt
     1561 aaaaataacg agaagttcag agcattacaa gagacatttg aatggagact cattaaatta
     1621 gtgattgcca tagttctaaa tattggaata atgtatatgc tgtataaaac aggaacattt
     1681 gatacccttt cacttacaaa cacaatagca atatcagtaa caccagctat tttaattctt
     1741 ctagcaatta ttggccagtt tgcaatatat tcaggagagc tgactgatgg acttgatggt
     1801 ttaatggttg gtatatttat aatcattaca actgcaatgt ttttccttct aacagttcaa
     1861 cagaaatatg aatacctgcc atttctagca ataacactgg gggtactcat agtagacctt
     1921 tactttaata ttcctcctgc aagattctgg aacggtggac ctggtgcaat gccaatctcc
     1981 tttgctatgt ttttcattgg attaacaaca ggaaatatta ttccatactt tttaatgtcc
     2041 tttataacat ggataataat gctatcaagt gcaatacaaa tgataagtat aaagttcttt
     2101 c
//
LOCUS       DAOSAB010000107         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_35878_length_2088_cov_2.849975, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000107 DAOSAB010000000
VERSION     DAOSAB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35878_length_2088_cov_2.849975"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..339)
                     /locus_tag="PK432_02310"
     CDS             complement(25..339)
                     /locus_tag="PK432_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59790.1"
                     /translation="MLYQLPNGKVIHLSVEQYLDLTDQDLQDMMAGNYGEYPTSHWID
                     SAIRSPRKQKEQRDIDKSIDYTEDSDELVIQSTTFAIITVDDINSSENIEDNSSEELE
                     DT"
     gene            complement(344..541)
                     /locus_tag="PK432_02315"
     CDS             complement(344..541)
                     /locus_tag="PK432_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59791.1"
                     /translation="MEETQNQAEPTSNKRAKKKFVYLEKYDLYKEGTDARIANIERSL
                     NVGFVVMGILVVWIILITINH"
     gene            complement(576..1052)
                     /locus_tag="PK432_02320"
     CDS             complement(576..1052)
                     /locus_tag="PK432_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59792.1"
                     /translation="MDEQRYVSITISSDDLKKMFMDKTKIDDNLATLIIGCLHNSNVG
                     IELLAKALFGIFPKIKYKLNDFVFIPVSNLPTWKTDRDSTLKLPSCKNECIMAQVVSI
                     DPYSTLTYKVKCTVVLTDGQVGFTDVLTHDRELQGTPEDPAEILGLIEDMKRELPF"
     gene            complement(1055..1324)
                     /locus_tag="PK432_02325"
     CDS             complement(1055..1324)
                     /locus_tag="PK432_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59793.1"
                     /translation="MEIEVNLDNLKVVPLGPNQYVIKSDGARIFQSYDSIIALKTDGM
                     VILDENKWDYSRTTGKYRNKFLDENKADTERRIKEGRYFLANLNK"
     gene            complement(1324..1536)
                     /locus_tag="PK432_02330"
     CDS             complement(1324..1536)
                     /locus_tag="PK432_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59794.1"
                     /translation="MQKKINLNNNNMAIVSEAVLKVIITDEDQNNVQVEFNAGKVPLD
                     AIIVSLEMVLERLKEPHKSQDRRNIN"
     gene            complement(1508..1657)
                     /locus_tag="PK432_02335"
     CDS             complement(1508..1657)
                     /locus_tag="PK432_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59795.1"
                     /translation="MEQFLLAIENHPKACAFLILLAILTCTFRFHWIVGKDVEEYAEE
                     NKSKQ"
     gene            complement(1659..1877)
                     /locus_tag="PK432_02340"
     CDS             complement(1659..1877)
                     /locus_tag="PK432_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59796.1"
                     /translation="MVVIETREQLEMFAWDIKMNMPDIPENLRLLIIMEPEAFERLVT
                     QISKEWVDNSDRFIYNSQVGIELGIKKG"
BASE COUNT          576 a          399 c          295 g          818 t
ORIGIN      
        1 ctctattacc gtggcctttg gtaattaagt gtcttctagc tcttctgagc tattatcctc
       61 aatattctct gaggagttaa tatcatctac tgtgattata gcaaaagttg ttgactgaat
      121 gactaattca tcactatctt cagtatagtc aatactttta tctatatctc tttgttcttt
      181 ttgtttgcgg ggggatctta ttgctgaatc aatccaatga gaagtagggt actctccata
      241 attacctgcc atcatatctt gtaaatcttg atcggttaaa tccaggtatt gctctactga
      301 gagatgtatt actttcccat ttggtaattg atacagcatt ttgctaatgg tttatagtaa
      361 ttagaataat ccaaacaact aatattccca ttaccacaaa acctacattt agagatcttt
      421 ctatgtttgc tattctagca tcagtacctt ctttataaag gtcatacttt tctagataaa
      481 caaacttttt cttagctctc ttattagagg ttggttctgc ctgattctga gtttcttcca
      541 tgattctttt ctttacacaa agatatactt tttttttaaa aaggtaactc tcttttcata
      601 tcttcaatta gccctagtat ttctgcagga tcttccggag ttccttgcaa ttccctatca
      661 tgggtgagaa catctgtaaa tcctacttga ccatcggtta aaactactgt acattttact
      721 ttataggtca aggtactata aggatctata cttactactt gagccattat acattcattc
      781 ttacagcttg gtaattttag agtagaatct ctatcagttt tccatgtcgg taaattactt
      841 actggtataa aaacaaaatc atttagttta tactttattt taggaaagat accaaaaaga
      901 gctttagcaa gcaattctat acctacattg gaattatgca gacaacctat tattaatgta
      961 gccagattat catctatctt ggttttatcc ataaacatct tcttaagatc atctgatgat
     1021 attgttattg atacatatct ttgttcatcc ataactattt atttaagttt gcaaggaaat
     1081 atcttccttc tttaattctt ctttctgtat ctgctttgtt ctcatccaag aacttgttcc
     1141 ggtacttacc ggttgtgcgg gaataatccc acttattctc atctaatata accattccat
     1201 cagtcttaag agctattatg ctatcatatg actgaaagat cctggctcca tcagatttta
     1261 tcacatattg attcggaccc agaggtacta ccttgagatt gtcaaggttt acttctatct
     1321 ccattaattt atgtttcttc tgtcttgtga cttatgtggt tcttttagcc tctctaaaac
     1381 catttctaat gatactatta ttgcatctag agggacctta cctgcattaa attcaacttg
     1441 cacattgttt tggtcctcat cagtaataat tactttgaga acagcttctg atactattgc
     1501 catgttatta ttgtttagat ttattttctt ctgcatattc ttctacatct ttccccacta
     1561 tccagtgaaa tctgaatgta caagtaagta tagcaagtaa tataagaaaa gcacacgctt
     1621 ttgggtgatt ttctatagct aataaaaatt gttccatatc atcctttttt tataccaagc
     1681 tctataccta cttggctatt gtaaataaat ctgtcagagt tatctaccca ctcttttgaa
     1741 atttgggtta ctaatctctc aaaagcctct ggctccatta ttattaggag tcttaaattc
     1801 tccggtatgt cgggcatatt cattttaata tcccatgcga acatctctaa ttgttctctt
     1861 gtttcaatta ctaccataat tttgttataa aggcacccag gttttcccgg gtgctcttgt
     1921 gttagatatc taaagcatat attggttcca ttattggtgc ttcaagtact ttttcaggta
     1981 tatcatcact gttagtattc aatacttcag ctaccctttt acctattaga tctggtgcag
     2041 gattattgca taatcctttg aattgtacag gtgtaaatat tactttat
//
LOCUS       DAOSAB010000108         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_36495_length_2063_cov_2.809761, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000108 DAOSAB010000000
VERSION     DAOSAB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36495_length_2063_cov_2.809761"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PK432_02345"
     CDS             <1..929
                     /locus_tag="PK432_02345"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOK59797.1"
                     /translation="NWAGSSWYYLRYCDPKNDKEFVSRQLMDYWMPVDHYEGGQEHIT
                     LHLLYSRFWHKVLYDLGLVRDVEPYKARSIHGVVLGEGGAKMSKSLGNVVNPDDLVEE
                     YGVDITRAYLMFMGPYDGNVQWSIRTIQGVRRFVSKYYVFLNRAYENRVEESNKSVKV
                     AIERLVNKIENDILDFKFNTAVAALMEFYNSYSKEKFDNKDLEKLVIASAPILPHISE
                     EIWCNTMGKKYSVHTQKWPEIDKELLEDEKIEIPVQINGKVRGKILISKESTQDQIQQ
                     IVLSSNTLGPLLSEKQIKKFVYVPQRIVSIVL"
     gene            1048..>2063
                     /locus_tag="PK432_02350"
     CDS             1048..>2063
                     /locus_tag="PK432_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59798.1"
                     /translation="MKKTNCPNLAKNLIILLILSLVFVVLFSGSIYALSSDNINLQGK
                     IVRNDTGHEGMNVIPGSPACVVNGPSNDTCDFRVSYYTALTGGTLLLTEDFTNVEIGQ
                     FDGVFELSLGSGTVTTTVQCRDGTCNSPSEVISEYGDLYIELQFAPDGSTLTETFTRM
                     PLEASPYAIFSKFAEGAHDAFKLSTSVSNESLENPSVGMIYFDTGSSQLKVYNGAEWQ
                     ELSAGTSSSLWTDAGTYSYLTSTTDDLVLGGSSFSTGRFVFDMDGGSGSYFDVFNNDH
                     SNKLFTILSSGNVGIGTATPGAKLEVGGTASTITNSTGNLTISTGGGNGNILLMPNGT
                     GNVG"
BASE COUNT          643 a          274 c          445 g          701 t
ORIGIN      
        1 ctaattgggc aggttcaagc tggtactatc tgcggtactg tgatccaaaa aatgataaag
       61 agtttgtatc tagacagcta atggattact ggatgcctgt agatcattac gaaggagggc
      121 aggaacatat tactttacat cttctttatt caagattctg gcataaggtg ctgtatgatt
      181 tggggcttgt tcgtgatgtt gaaccttaca aagcaagaag tattcatggt gtagtacttg
      241 gagagggtgg tgctaagatg tctaagtcat tgggcaatgt tgttaatcct gatgatcttg
      301 ttgaggaata tggtgttgat attaccagag cctatcttat gttcatgggt ccatatgatg
      361 gaaatgttca gtggagtata agaacaattc aaggtgttag aaggtttgtt tctaagtatt
      421 atgtattttt aaatagagca tatgagaaca gagttgagga gagtaataaa agtgtaaaag
      481 tagctattga gagattggta aataaaattg agaatgatat tctagatttt aagttcaaca
      541 ctgcagttgc tgcactcatg gaattttata actcatactc taaagagaaa tttgataaca
      601 aagacctaga gaaattagta attgcttctg caccaatact tcctcatatt tctgaggaaa
      661 tatggtgtaa tactatgggt aaaaaatatt cagttcatac tcagaaatgg ccagaaatag
      721 ataaagagct tttagaagat gaaaaaatag agattcctgt acaaataaat gggaaggtta
      781 ggggaaagat actcatatct aaagagagta cacaggatca gatacagcaa attgtcctct
      841 catccaatac tctaggtcct cttttatcag agaaacaaat taaaaaattt gtttatgttc
      901 ctcaaaggat agtgagtata gttttgtaag gggtaaatat agtcaaaagc cgtttgacag
      961 gtgtacacat ttctactatt attataatac agatttaaag tacttaaaaa tactgttaat
     1021 acaagacaaa agtacgatct gttgagtatg aaaaaaacaa actgcccaaa cttagcaaaa
     1081 aatttaatca ttttgctgat tttatcctta gtttttgtgg ttcttttctc aggttcaata
     1141 tatgcccttt cgtctgataa tattaactta cagggtaaga ttgtaagaaa tgatacaggc
     1201 catgagggta tgaatgttat ccctggctct ccagcatgtg ttgttaatgg accaagtaat
     1261 gatacttgtg attttagggt ctcatattat actgcattga ctggaggcac tcttcttttg
     1321 acagaggatt ttactaatgt tgagattggt cagtttgatg gtgtttttga actttcactt
     1381 ggaagtggaa ctgttacaac aacagtacag tgtagagatg gtacatgcaa ctctccttct
     1441 gaggttattt ctgagtatgg agatttatat attgaactac aatttgctcc tgatgggagt
     1501 actcttactg aaacttttac aagaatgcct cttgaggcta gtccgtatgc gatattttca
     1561 aagtttgcag agggggcaca tgatgctttt aaattaagta cttctgttag taatgagtct
     1621 ttagagaatc cttctgtggg tatgatttat tttgatactg gttcttctca gcttaaagtt
     1681 tacaatggtg cagagtggca ggaattgagt gctggtactt cttcttctct ttggactgat
     1741 gctggtacat attcatattt aacttctact acagatgatc ttgtattagg gggtagttca
     1801 tttagtactg gtaggtttgt ttttgatatg gatgggggta gtggtagtta ttttgatgtt
     1861 tttaataatg atcattcaaa taaattattt actattttaa gtagtggaaa tgttggtatt
     1921 ggtactgcta ctcctggggc taagttagag gtaggaggaa ctgcttctac aataactaat
     1981 tctacaggta atttaactat atcaacagga ggaggtaatg gaaatatatt acttatgcct
     2041 aatggtacag gaaatgttgg aat
//
LOCUS       DAOSAB010000109         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_37425_length_2026_cov_3.754947, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000109 DAOSAB010000000
VERSION     DAOSAB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37425_length_2026_cov_3.754947"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /gene="topA"
                     /locus_tag="PK432_02355"
     CDS             complement(<1..1135)
                     /gene="topA"
                     /locus_tag="PK432_02355"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOK59799.1"
                     /translation="MKNSKEYRNLLIVESPAKAKTINRYLGDDFKVMATVGHVIDLPK
                     SELGVDVEKGYEPLYQVIHGKKKILQELKRALPEQGNVYLAMDPDREGEAIAWHVKEY
                     LKLKNAKRVSFHEITKDAILEAIKSPSTVNEDLVEAQKARRVLDRLYGYKLSELLWKK
                     IWYGLSAGRVQSVALRLVVEREEEREAFKPEEFWDFLVEVSKNGEKLKIKLIKKDGKK
                     YIPKKEQEVKEIEEMLDNHAYKVVDIVKKEIRRNPYPPYTTSTLQQSANNVLGFSAKK
                     TMTIAQRLYQAGYITYMRTDSVYLAQKAIKEIRGVIADKYGKQYLPEKERYYKNKSKN
                     AQEAHEAIRPTDFSFDSSKLSSDEAKLYNLIWKRAVSSQMNSEI"
     gene            complement(1223..>2026)
                     /locus_tag="PK432_02360"
     CDS             complement(1223..>2026)
                     /locus_tag="PK432_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOK59800.1"
                     /translation="TRYLSRTGYTFSNGAPFKEFENHAYGSLNVVDALMVSSNIFFCE
                     MIRDWNMNELVPYLERFGIGQYTGVDLPGEMSGRLPSPENKRKLAQTISPWLEEVWYP
                     EGDSCNSVIGQGITLVTPIQMSNWIAAIANGGTLYTPHVGKKFVDENGLEFPIEYDVI
                     RENIVSDKSLKIVREGMWSVVNGSRGIAKALSTAGVTVAAKTGTAEFGKVNEKGEYED
                     THAWVTGFFPYENPKYSFSLLLEDGGTSANAVATIKEIITWMVQNGLAD"
BASE COUNT          588 a          450 c          263 g          725 t
ORIGIN      
        1 caatttcaga attcatctga gaagataccg ctcttttcca tatcaaattg taaagcttag
       61 cttcatcaga agaaagtttt gatgaatcaa atgagaagtc tgtaggtctt attgcctcat
      121 gtgcttcttg tgcattctta gatttatttt tgtaatatct ctctttctca ggtagatact
      181 gtttaccata tttatctgca attactcctc ttatctcttt aattgccttt tgtgctaaat
      241 acacagaatc tgttctcatg tatgtaatgt aaccagcctg atacaatctt tgagctatgg
      301 tcattgtctt tttagcacta aacccaagaa cattattagc agattgctga agagtagatg
      361 ttgtatatgg tggatatgga ttccttctaa tttctttctt tacaatatct actaccttgt
      421 atgcatgatt atcaagcatc tcttctattt ctttaacttc ctgctctttt ttaggtatat
      481 actttttccc atcttttttt atcaatttta tctttaactt ctcaccattt ttacttacct
      541 ctacaagaaa atcccaaaat tcttcaggtt tgaatgcctc tctctcttcc tctcgttcta
      601 caactaatct aagtgccaca gattgaactc ttcctgctga aagtccatac cagatttttt
      661 tccaaagaag ctccgaaagt ttatacccat acaatctgtc taacactctt ctggcttttt
      721 gagcctcaac aagatcttca ttcactgtac taggagattt aatagcctcc aagatagcat
      781 ccttggttat ttcatggaaa gaaactcttt tagcattttt aagcttaaga tactccttaa
      841 catgccaagc aatagcctct ccttctctat caggatccat tgcaagatat acattgcctt
      901 gctctggaag tgctcttttt aactcttgca gaatcttttt tttgccatgt ataacctgat
      961 acaaaggttc atatcctttt tcaacatcta cccctagctc acttttaggc aagtcaatca
     1021 catgaccaac tgttgccata actttaaagt catcaccaag atacctgttt atagtttttg
     1081 cctttgcagg agactctaca atcaacaaat ttctatactc cttcgaattt ttcatattcg
     1141 ctgtaaaaat acacaatgtt taaatatatg tcaacaaaac catacattca ctttaatata
     1201 ttaaagtgaa aaaatcctta acttaatctg ccaatccatt ctgcaccatc catgtaatta
     1261 tttcttttat cgttgctact gcattagcac ttgtacctcc atcttctaaa agaagagaaa
     1321 atgaatattt aggattttca tacgggaaga agcctgtaac ccatgcatgt gtatcctcat
     1381 actcaccttt ctcattaact ttaccaaact ctgctgtacc agtttttgct gcaacagtta
     1441 cacctgctgt agataacgcc ttagcaatac ctctagaacc attaactaca ctccacattc
     1501 cttctctaac tatctttaaa gacttgtcag atacaatatt ttccctaatt acatcatatt
     1561 caattggaaa ctctaaaccg ttttcatcaa caaatttttt accaacatga ggagtataca
     1621 gtgtaccgcc gtttgcgatt gcagcaatcc agttagacat ttgtattggt gtaaccaaag
     1681 ttataccctg ccctattact gagttgcatg aatctccctc tggataccat acttcttcaa
     1741 gccatgggct tattgtttgt gcaagctttc ttttattctc aggagaagga agcctaccag
     1801 acatttcacc aggtagatct actcctgtgt actgtcctat accaaatctt tcaagatatg
     1861 gaaccaattc gttcatgttc caatctctaa tcatttcaca aaagaaaatg ttagaggaca
     1921 ccattaaagc atcaacaaca tttaaagagc catacgcatg gttttcaaat tctttaaatg
     1981 gtgctccatt agaaaatgta taacctgttc tacttaaata tcttgt
//
LOCUS       DAOSAB010000110         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_37569_length_2020_cov_7.247837, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000110 DAOSAB010000000
VERSION     DAOSAB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37569_length_2020_cov_7.247837"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2020)
                     /locus_tag="PK432_02365"
     CDS             complement(<1..>2020)
                     /locus_tag="PK432_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59801.1"
                     /translation="NQSNVLYQLLQDREIEEFVASEKTIRDLAARMSDRIGIPVRFEF
                     DRTKEYKGKLDQGTADEYEQTVAVINLAYATLDTPIHEILGHPIIRVIKNTNPQLYQN
                     LLKELEYGRGKEVLDRVKRDYNIKGDYTIFQMSDGMWSVIDNNNVEKPFGRYGTKEMA
                     VSGLPSYTLEEQQEEALVQLLGLMTAGKLDSVKDGKLISLLKRLLKEIKSFVRDLLKQ
                     REVEIDKLPDNMTIGDLSDLLAYSNSKLILPGYEVQYTTPDNQKFSTYAEASKHISEL
                     VKSAKDIDLNKVKIQTYNRNKPLNDFYVIDSGRYYIKEDKFYFDSWNGTTYELTKEQF
                     LSGWNNYLHNSVFNFIEKNREYEQSKEIIEEWKKVNNIQYNPEEIYSRGQEFCSVVGA
                     YSLFDVNLMMQNLLQHIEDNEKAGGKFAISAFTKPIGKTIRHLEGRGGKIKFKIYPKS
                     EDILWASNIDAHSGSVWDASEKINEDKKSELLGVSFTKYPSLRKIDSVQPNLASIVDD
                     LAYHHNELGIVLTGNNFRLEYDEDVPYSTKKLINSINSILDQKYGKLVKPEITLEKRG
                     GIQPTQTNETLKESIESVTARVLQDKKEYTEQALINTKIAKLKEVAKKYPRSLIRSEV
                     KPIRSSAFSDSVMYDAVFDPDELPFQKASGSIQYQKQIDLQAEQDAVLE"
BASE COUNT          626 a          348 c          240 g          806 t
ORIGIN      
        1 ttctaagaca gcatcttgct ctgcctgtaa atctatttgt ttttgatatt gaatactacc
       61 acttgccttt tggaatggta gttcatcagg gtcaaatact gcatcataca ttacactatc
      121 agaaaaagct gatgatctta taggtttaac ttcacttcta attaaacttc tagggtactt
      181 cttagctact tctttaagtt tagctatttt agtatttata agagcttgtt cagtgtattc
      241 ttttttatct tgcaatactc ttgctgtaac actctcaata ctctctttta aagtttcatt
      301 agtttgagtt ggttggatgc ctcctctttt ttctaaagta atctcaggtt taaccaactt
      361 accatacttc tggtccagta tagagttaat actattaata agttttttag tagaatatgg
      421 aacatcttca tcatattcta atctaaagtt atttcctgtt aaaacaatac ctaattcatt
      481 atgatgatat gctaaatcat caacaataga agctagatta ggttgtactg aatcaatctt
      541 tcttaaagaa ggatatttgg taaaagacac tccaagcaat tcagattttt tatcttcatt
      601 aattttttcc gaagcatccc atacactccc agaatgagca tcaatattag aagcccataa
      661 tatatcttct gatttaggat aaattttaaa tttaatttta ccacctcttc cttctaaatg
      721 acgaatagtt ttaccaatag gtttagtaaa agcagaaata gcaaatttac ctccagcttt
      781 ttcattgtct tctatatgtt gtaatagatt ttgcatcata agatttacat caaataatga
      841 ataagctcct acaacagaac aaaattcttg acctcttgaa tatatctctt cagggttata
      901 ttgaatatta tttacttttt tccattcttc tataatttct ttagattgct catattctct
      961 gtttttttct ataaaattaa acacagaatt gtgtagataa ttattccaac ctgatagaaa
     1021 ttgttctttt gttagttcat atgtagtacc attccaacta tcaaaataaa atttgtcttc
     1081 tttaatataa tatctacctg aatctattac ataaaaatca tttagtggct tatttctatt
     1141 atatgtttgt attttaactt tattcaaatc aatatcttta gcacttttaa caagttcact
     1201 aatatgttta cttgcctcag catatgtact aaacttctga ttatccggtg tagtatattg
     1261 tacttcatat ccgggcaata taagtttaga attagaataa gctaaaaggt cagataaatc
     1321 acctattgtc atattatccg gcaatttgtc tatttcaact tctctttgtt taagtagatc
     1381 tctaacaaaa gatttaattt ctttaagaag tcttttaagt agagatatta atttaccatc
     1441 tttaacacta tctagtttac cagcagtcat taaacctaat aattgtacaa gagcttcttc
     1501 ttgttgttct tctaaagtat aacttggtaa accagagaca gccatttctt tagtaccata
     1561 cctaccaaaa ggtttttcta cattgttgtt atcaattaca ctccacatac catctgacat
     1621 ctgaaatata gtataatccc ctttaatatt ataatccctc ttaaccctat ctaatacttc
     1681 tttgcctcta ccatattcga gttcttttag tagattttgg tatagttgag gatttgtatt
     1741 ttttataact ctaataatag gatgtcctaa tatctcatgt ataggtgtat ctaaagtagc
     1801 ataagctaaa tttattacag ctacagtttg ttcatattca tctgctgtac cttggtctaa
     1861 tttaccttta tattctttag tcctatcaaa ttcaaatctt acaggaatac ctattctatc
     1921 agacattctt gctgctaaat ctctgatagt tttttcagaa gctacaaatt cttctatttc
     1981 tctatcttgt agtagttgat atagaacatt tgattgatta
//
LOCUS       DAOSAB010000111         2019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_37614_length_2019_cov_2.965886, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000111 DAOSAB010000000
VERSION     DAOSAB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37614_length_2019_cov_2.965886"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PK432_02370"
     CDS             complement(<1..1083)
                     /locus_tag="PK432_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOK59802.1"
                     /translation="MNESLKSDLEGQRKVRLEKLQKIKELGINPYPSKSFRDIQIGDI
                     KGEFEKYEGSVHTVAGRITGWREHGKLIFADIKDPSGTIQIILKLEWLKKGLDEGYLQ
                     WEHLELLDLADFIEVTGKVGKSKTEEISILGESVRLLTKALRPIPRKLLDKEQKFRRR
                     YLDLTINPERIELFKRKAKFWEIQREFLKNEGFIEVETPVLENVTGGADAKPFITHHN
                     ALDIDLYLRISTELYQKRLIGGGFEKVYTFGPNFRNEGMDDEHLPEFYDIEWYWAYAN
                     FKDNMELVKRLFLEIAQKIYGRTKFTTRGHTFDLADEWEEIDYTEALKKNFNVDILNT
                     PEEDMLKAIKDKGLEVEEGMNRNRMTD"
     gene            complement(1135..>2019)
                     /locus_tag="PK432_02375"
     CDS             complement(1135..>2019)
                     /locus_tag="PK432_02375"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOK59803.1"
                     /translation="TVIPFLVTCILLLLGILTTLIKLSSKTSPQSEYIPSNVSMVSAM
                     EVGQAEELKEENEKDKTFSVSVCDFSSCVWISSEGLVNNGIVDQNAVYQKVLDSVIPY
                     FENKHGGKTFVKNRGGEFIYWRYDNVPDFSNIYDKVYEAFKSGVNSRVEVEIKDMPGT
                     DGRYADKYIEVDDSKLKLYAWVDGKVVKEIDLSPAKKGYQVYGVFPIIDKGVAPIAPG
                     GKYMPYWMAFYYSPKQDSYYGLHGLIWWYDENGKKVYETEEHIGVRRSKGCIRMTVEN
                     AKFLYNIYQRGDPVLIHE"
BASE COUNT          611 a          454 c          223 g          731 t
ORIGIN      
        1 atctgtcatt ctgtttctgt tcataccttc ttcaacctcc aaacctttat ccttaatagc
       61 ttttaacata tcttcctcag gagtatttaa tatatcaaca ttaaaattct tttttaatgc
      121 ctctgtataa tcaatctcct cccattcatc tgctaaatca aatgtatgac ctcttgttgt
      181 aaacttagtt cttccatata tcttttgagc tatctctaaa aacaatcttt ttaccagctc
      241 catattatct ttgaaattag catatgccca ataccattca atatcataga actctggaag
      301 atgctcatca tccataccct catttctaaa attaggacca aatgtataaa ccttttcaaa
      361 ccctccccct ataagtcttt tttgataaag ctcagtagat attctaaggt acaaatcaat
      421 atccaaagca ttatgatgag ttataaatgg cttagcatca gctcctcctg ttacattttc
      481 taaaacaggg gtttcaactt caataaagcc ttcgttttta agaaactctc tttgaatttc
      541 ccaaaacttt gcctttcttt taaataactc tattctttct ggattaatag ttaggtcaag
      601 ataccttctt ctaaacttct gctctttatc aagaagtttt ctaggtatag gcctgagtgc
      661 ctttgtaagc agtcttacac tttctccgag tattgatatt tcctctgttt tacttttccc
      721 aacctttcct gttacttcta taaaatcagc aaggtctaaa agctcaagat gctcccattg
      781 taaatatccc tcatctaaac ctttcttaag ccactctaat ttaagtatta tctgaatagt
      841 accgcttgga tctttaatat ctgcaaatat taatttgcca tgctctctcc atcctgttat
      901 tcttccagca acagtatgta cacttccttc atatttctca aactccccct ttatatctcc
      961 aatctgtata tctctgaatg attttgaagg atatggatta attccaagct ctttaatttt
     1021 ctgaagcttt tctaatctta cttttctttg tccctctaaa tcacttttta aagactcatt
     1081 cattggatag tttaaacaat taatatattc cccgattata tcacacaatc tttactattc
     1141 atgtataagg acaggatctc ccctctgata tatattgtaa agaaattttg cattctcaac
     1201 agtcatacga atacatcctt tgcttcttct tacaccaata tgctcttctg tttcatatac
     1261 ctttttgcca ttctcatcat accaccagat aagaccatga agaccatagt aggagtcctg
     1321 tttaggagag tagtagaaag ccatccagta aggcatatac ttgccaccag gagcaatagg
     1381 agcaactcct ttatcaataa ttggaaaaac accatatacc tgatatccct tctttgcagg
     1441 agataaatca atctccttaa caaccttgcc gtctacccat gcataaagtt ttaactttga
     1501 atcatctacc tcaatatatt tatctgcata ccttccatca gtacctggca tatcctttat
     1561 ttcaacctct acacgactgt taactccgct cttaaaagct tcatatactt tatcatagat
     1621 attactaaaa tctggtacat tatcatatct ccaatagata aactctcctc ctctattttt
     1681 tacaaatgtc ttacctccat gcttattttc aaaatatgga attacactat ctaacacttt
     1741 ttgataaaca gcattttgat caacaatccc attattaacc aatccttcac tagatatcca
     1801 tacacaactt gagaaatcac atacagaaac tgaaaatgtt ttgtccttct cattctcttc
     1861 tttcaactct tctgcctgcc caacctccat agctgatacc atagaaacat ttgaaggtat
     1921 atattctgat tgaggagagg ttttagaaga gagtttaata agtgtagtta aaattcctaa
     1981 taatagtaaa atacatgtta caagaaatgg tattactgt
//
LOCUS       DAOSAB010000112         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_38063_length_2002_cov_2.400103, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000112 DAOSAB010000000
VERSION     DAOSAB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38063_length_2002_cov_2.400103"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PK432_02380"
     CDS             complement(<1..918)
                     /locus_tag="PK432_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59804.1"
                     /translation="MKKIVKILIPLLLIVPLFLSSTFVNGQESRKFAVSFTGIGCPHC
                     AQVSPQLHSRVQNGNFILIEYEIYSNVANSQAFSTYAQSFNLDLGIPQVLFNKDMKGV
                     GDAPIIENIDSMINKAVANEIPLADGRVISFEDFNLNSLVRYPTIYSKDRVAIRKSIT
                     QLSEEENEQIKTFIYSSSINDAVSSLKGSSTKAEIVKTPGGNFTYDHAVKLNGWLLQW
                     NGDAIPVVENTEETKTNDEGIVKNQEEKLSIGKIISLGLADSVNPCALSILALVLISI
                     ITYNPGKRKDILFAGLSFILAVIIMYLLYG"
     assembly_gap    988..997
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <998..1756
                     /gene="map"
                     /locus_tag="PK432_02385"
     CDS             <998..1756
                     /gene="map"
                     /locus_tag="PK432_02385"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOK59805.1"
                     /translation="INSQRERDILTKAADISVNILKQVGENIREGITPLELDNIAGEL
                     CRKYKVKPNFKKVEGYHFNTCISVNDVAVHGIPNSIPLKRGDLVSIDFGIVYKGFNTD
                     HCWTWSIGQPSDKNRKLLLAGRKAVENCLPLAITGNYTGDLGYEMERVAKENGFNVLR
                     MFVGHGIGKSLHEEPDIPAYGRKHTGSRLITGMVICIECQVVDDDGNIKIDSDGWSAR
                     TSHGGNSVMFEYMVIVDNKYPTVLTNTLDWDFIA"
BASE COUNT          666 a          267 c          357 g          702 t
ORIGIN      
        1 accataaagc aaatacataa taattacagc taagataaaa gaaagacctg caaaaagaat
       61 atcttttctc tttccagggt tgtaagtgat aattgaaatt aaaacaagag caagtataga
      121 tagtgcacag gggtttacag aatcggcaag accaagtgaa attatttttc caattgataa
      181 tttttcttct tgattcttaa caattccttc atcattggtt tttgtttctt cggtgttttc
      241 tacaacaggt attgcatcac cattccattg taaaagccat ccattaagtt taactgcatg
      301 gtcatatgta aaatttccac ctggtgtttt tactatttct gcttttgtac tacttccttt
      361 aagagatgaa actgcatcat ttatggaaga ggaatatata aaggttttaa tttgctcatt
      421 ttcttcctca cttaactgtg ttattgattt ccttattgca accctgtcct ttgagtatat
      481 agtgggatat cgaacaagag agttaagatt aaaatcttca aaagaaatta ctctaccatc
      541 agctaatggt atttcatttg ctactgcttt attaatcatt gagtcaatat tttcaattat
      601 gggagcatct cctacccctt tcatatcttt attaaacaaa acttgaggaa tacctaaatc
      661 aagattaaat gattgtgcat atgtactaaa tgcttgtgag ttagccacat tgctatatat
      721 ttcatattca ataagaataa aattcccatt ttggacccta ctatgaagct gaggagacac
      781 ctgagcacag tgaggacatc ctatacctgt aaatgataca gcgaacttcc gactttcttg
      841 tccatttaca aaagtgcttg aaagaaacaa ggggactatt aatagtaagg gtattaatat
      901 ttttacaatc tttttcattt aagtaacagt atttaagtca gtttttagtc tagtagtcag
      961 tatatcatac tttcattgtt agtaagannn nnnnnnnatt aattcacaaa gagaaagaga
     1021 tattttaaca aaagcagcgg atatatctgt aaatatatta aaacaggttg gagaaaatat
     1081 tagggaaggt attactccac ttgagcttga taatattgca ggtgagctgt gcaggaagta
     1141 caaagtaaaa cctaatttta agaaagttga aggatatcat tttaatacat gtatttctgt
     1201 aaatgatgtt gctgtgcatg gtattcctaa ttcaattcca cttaaaagag gagacttagt
     1261 atctattgat tttggaatag tatataaagg atttaatact gatcactgct ggacatggag
     1321 tataggtcaa ccaagtgata agaatagaaa actgttatta gcaggaagaa aagcagtaga
     1381 gaattgtctt cctcttgcta ttactggcaa ctatactgga gatttaggat atgagatgga
     1441 aagggttgca aaagagaatg gttttaatgt tttaaggatg tttgttgggc atggtattgg
     1501 gaaatctctt catgaagagc cggatattcc tgcttatgga agaaagcaca caggtagtag
     1561 attaattact ggtatggtta tatgtattga atgtcaggtt gttgacgatg atggaaatat
     1621 taaaatagat agtgatggat ggagtgcaag gacttctcat ggtggtaata gtgtaatgtt
     1681 tgaatatatg gttatagttg ataataaata tcctactgtt ttaacaaata cacttgactg
     1741 ggattttatt gcttagaatc tttattaccc tatagtatta tggtataata taagtaggta
     1801 ttcttaatac cttttttcct tttgttttaa tacattagga aatactgctc tttaaaaggt
     1861 ctacttatat ttttcttctc tattttaaca gccttaaatg catatatatt aagtttgtat
     1921 atatttatgg ctgttaaaaa tcattaaaac aatgaaaagg agaagaaatg agaaagtatt
     1981 tgattgttgt atgtgttatt gt
//
LOCUS       DAOSAB010000113         1979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_38711_length_1979_cov_2.252079, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000113 DAOSAB010000000
VERSION     DAOSAB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38711_length_1979_cov_2.252079"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1979)
                     /locus_tag="PK432_02390"
     CDS             complement(<1..>1979)
                     /locus_tag="PK432_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59806.1"
                     /translation="EKQAEEIMKSVYERAKANIEAISKDIHSKNYTEDIKELKLQQLK
                     YLKTIVRNIEEQKDKEDKSLFRGLIFAKSLATIKDEITRINTEFSNINEQYTKALTEY
                     GKNPTEGNKRDYIKLYSKLLELHNQNKAAGDALGILYEIAINIKNNPKNNISEDSPIF
                     KNVLSAYAQYNSILNDFRDATIPMIVNVLKGIGKESFENIRLELTDAFMVEQKRINKR
                     LAKLKQGKLGLIRRGAMRVAGVINKDLKEQYSDISPDNNSEIALEIARLEKRKDQLNQ
                     ILAGLKYDDTALSTIVNNMKDPTSILYLGNRTLYTQNGGIDIIDFSKFQATITDSDLG
                     VASVMIMLKNAEGMMHDNLVNDKGLHNLANATDSILSKKGLTIEQLNDLISGRRNVTY
                     YNINTGEVTTSNQLYLVKPMSEEYENTYRKFQSDARAFKEAEKLIRAKYNKAIKTGNA
                     EEIAALKREYDAAKQKSYEHTKAMISWMLENCNLPYKDAYYKLQAGLPDHYKQQIDDI
                     YLQMYIISSEDLMMQSDDDADMLLTLKAEIRRIKQLAKEENTEYARIIEQMENMYEPV
                     YNEARYNAMLKNAEIQFADYPEQLAKWKEKYQITRPNSEWFKKRAEIYDEIASIYGNS
                     TDDELSNLFRQRRDIMNRFTFNKIFNPRIMTSDDI"
BASE COUNT          600 a          305 c          210 g          864 t
ORIGIN      
        1 tatatcatca gaagtcataa ttctaggatt aaatatctta ttaaaagtaa acctattcat
       61 tatgtctctt ctttgcctaa ataaattaga taattcatca tcagtactat taccatatat
      121 tgatgcaatc tcatcatata tttcagctct ttttttaaac cattctgaat taggtcttgt
      181 aatttgatac ttttctttcc atttagctaa ttgttcagga taatcagcaa attgtatttc
      241 tgcattttta agcatagcat tatatctagc ttcattataa acaggttcat acatgttttc
      301 catttgttct ataatcctag catactctgt attttcttct ttggctaatt gtttaatcct
      361 tctaatttca gcttttaatg ttaataacat atcagcatca tcatctgatt gcatcattaa
      421 atcttcagat gatataatat acatttgtaa ataaatgtca tctatttgtt gtttgtaatg
      481 atcaggcaat ccagcttgta gtttgtaata agcatcttta taaggaagat tacaattttc
      541 tagcatccaa ctaatcattg cttttgtatg ttcatatgac ttttgtttag cagcatcata
      601 ttctcttttt aaagcagcta tttcctctgc atttcctgtc ttaatagctt tattatattt
      661 agctctaata agcttctcag cctctttaaa agctcttgca tcagattgaa attttctata
      721 agtattttca tattcttctg acattggttt tactaaatat aattgattag aagttgttac
      781 ttctcctgtg tttatattat aataagttac attccttctg ccagaaatta aatcatttaa
      841 ttgttcgatg gttagtcctt ttttagataa tatagaatct gtagcattag caagattgtg
      901 tagtccttta tcattgacta aattatcatg catcatacct tctgcatttt ttaacataat
      961 cataacagaa gcaactccta agtctgaatc agtaatagta gcttggaatt tagaaaaatc
     1021 tataatatca ataccaccat tttgagtgta taatgttctg ttaccaagat ataatattga
     1081 agtaggatct ttcatattat ttactatagt agataatgct gtatcatcat attttaatcc
     1141 tgctaatatt tgatttaact gatcttttct tttttctaat cttgcaatct ctaatgcaat
     1201 ttctgaatta ttatcaggac taatatcaga atattgttct tttaagtctt tattaataac
     1261 tccagctaca cgcattgcac cccttctgat gagtcctaat tttccttgtt ttaattttgc
     1321 aagtctctta ttaattcttt tctgttctac cataaaagca tcagttaact ctaatctaat
     1381 attttcaaaa gattctttac caataccttt taatacatta acaatcattg gaatagtagc
     1441 atctctaaaa tcatttaata tagaattata ttgcgcataa gcagataata catttttaaa
     1501 aataggacta tcttctgata tattattttt aggattattt ttaatattaa ttgctatttc
     1561 atataaaatt cctaaagcat caccagcagc tttattttga ttatgtaatt ctaataattt
     1621 agaatataat tttatataat ctctcttatt tccttctgtt ggattttttc catattcggt
     1681 taatgctttt gtgtattgtt catttatatt agaaaattct gtattaatcc ttgttatttc
     1741 atctttaata gtagctaaag atttagcaaa aattaatcct ctaaataatg atttatcttc
     1801 tttatctttt tgttcttcta tatttcttac aattgttttt aaatatttta attgttgtaa
     1861 ctttaattct tttatatctt ctgtatagtt tttagaatga atatctttag aaatagcttc
     1921 tatatttgct ttagctcttt cataaacaga tttcattatt tcctctgctt gtttttcat
//
LOCUS       DAOSAB010000114         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_38728_length_1978_cov_3.121165, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000114 DAOSAB010000000
VERSION     DAOSAB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38728_length_1978_cov_3.121165"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PK432_02395"
     CDS             <1..867
                     /locus_tag="PK432_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOK59807.1"
                     /translation="KPRKSTLLKEWEAKTRKMKKISKSVDIGIVGKYYKSGKFSLKDS
                     YVSVLEAINHAGWEMGVNPNIHWIVADDLENNKELQKEILKMDGIIVPQGWGSRGAEG
                     KIKAIQIARENEIPYLGLCYGMQMATIEFCRNVLGIKDANSEEIDPKSKNLVIHLMEN
                     QKEILKHQNYGGTIRLGAWPCKIKEGTLLESLYKKYTNSLYNTLPVVMERHRHRYEFN
                     TKYSSLIESKGMIISGESPDGKLVEAIELNKTLHPFFIGTQYHPELKSRFLAPHPIFL
                     GFITAAKKRKII"
     gene            886..1746
                     /locus_tag="PK432_02400"
     CDS             886..1746
                     /locus_tag="PK432_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOK59808.1"
                     /translation="MEVKKAVITTAGYSTRFLPACKNIPKVMLPILEVPIIHEQVKEC
                     IEGGITDIIIITSYGNNAIEDYFDSRPDLETHLVNTGKEDRYKRFGEVFGKANVVCVR
                     QNRSLPYGSGSAILAAQPWIQKSEPFAILYGDDLVLSKKSGIGQLKEFFESQTSIGGV
                     IAVQKVDEKDISKYGCVRFRDESKGIIDSILEKPSVNEAPSLLGSYGRYIVSYDIFKY
                     LHTGTLINNELYFSKAIDDMAKETDVYAKMIDGEWLTTGDPHNYLNAVIRYALQREDY
                     REEVTVTVNS"
     gene            complement(1736..>1978)
                     /locus_tag="PK432_02405"
     CDS             complement(1736..>1978)
                     /locus_tag="PK432_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOK59809.1"
                     /translation="ILVPESIKNITEKLNATKVVKIIDGYRGKPPLPKQKLIEMIDKV
                     QRLLVTYPQIYSIDMNPVILTESRVVAVDLKIYIKS"
BASE COUNT          716 a          250 c          397 g          615 t
ORIGIN      
        1 aaaccaagaa aaagtacatt attaaaagag tgggaagcaa aaactaggaa gatgaagaag
       61 atttctaaaa gtgtggatat tggaattgtt ggaaaatatt ataaaagcgg gaaatttagt
      121 cttaaagact catatgtatc agtacttgaa gcaattaatc atgcaggctg ggaaatggga
      181 gttaatccta atattcactg gattgttgct gatgaccttg aaaataacaa ggaattacag
      241 aaagagattc ttaaaatgga tggaataatt gttcctcagg gctggggaag tagaggtgca
      301 gagggaaaga taaaggcaat acagatagca agagaaaatg aaataccata tctagggttg
      361 tgctatggta tgcagatggc aactattgag ttttgtagaa atgtacttgg tattaaagat
      421 gcaaattcag aggaaataga tcctaaatca aagaatttag ttattcattt aatggaaaat
      481 caaaaagaga tacttaagca tcagaattat ggtggtacga taagactggg agcatggcca
      541 tgtaaaataa aggaaggaac attactggaa tctctttata aaaaatatac caattcgttg
      601 tacaatactc ttcctgttgt aatggaaaga cacaggcata gatatgagtt taatacaaaa
      661 tattcttcat taattgagag taaaggcatg ataatttcgg gagagagtcc tgatggaaaa
      721 cttgtagaag caattgaact taataagact ttacatccat tttttattgg aacacagtat
      781 catccagagc taaagagtag atttttagct ccacatccaa tatttttagg ttttataact
      841 gctgctaaaa agaggaaaat tatttaatat attaactatg tttttatgga agtaaagaaa
      901 gctgttatta caactgcagg atattcaact agatttcttc ctgcatgtaa aaatattcct
      961 aaggtgatgc taccaattct agaagtccct attattcatg aacaggtaaa agagtgtatt
     1021 gagggaggaa ttacagatat tataattatt acatcatatg gaaataatgc tattgaggat
     1081 tattttgaca gtagacctga tttagaaaca cacttagtta atacaggcaa agaggataga
     1141 tacaaaaggt ttggagaggt atttggaaaa gcaaatgtag tttgtgtaag acagaacaga
     1201 tctcttccat atggaagtgg aagtgcaatt cttgcagcac agccttggat acaaaagagt
     1261 gaaccatttg caattcttta tggagatgac cttgttttat caaaaaagtc aggtattgga
     1321 cagttaaaag aattttttga gtcacaaacc agtatcggag gggttattgc tgttcaaaaa
     1381 gttgatgaaa aagacatatc aaagtatggg tgtgtaagat ttagggatga gagtaaagga
     1441 attattgatt ctatacttga aaaaccatct gtaaatgaag ctccttctct tcttggctca
     1501 tatggaagat atattgtttc ctatgatatt tttaaatatc ttcatacagg tacacttatt
     1561 aataatgaac tgtatttttc aaaagcaatt gatgatatgg caaaagaaac agatgtttat
     1621 gctaagatga ttgatggaga gtggttaaca acaggagacc cgcacaatta tttgaatgct
     1681 gtaataaggt atgcattgca aagagaggat tacagggaag aagtgactgt tacagttaac
     1741 tcttaatata tatttttaag tcaactgcga ctaccctact ttctgttagt attacaggat
     1801 tcatatcaat gctgtaaatt tgggggtatg taacaagaag tctttgtact ttatctatca
     1861 tttcaataag cttctgctta ggaagtggag gttttcccct atatccatca attattttta
     1921 caacctttgt agcatttaat ttctctgtta tattctttat actctcagga actaatat
//
LOCUS       DAOSAB010000115         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_39677_length_1942_cov_3.210917, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000115 DAOSAB010000000
VERSION     DAOSAB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39677_length_1942_cov_3.210917"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PK432_02410"
     CDS             complement(<1..522)
                     /locus_tag="PK432_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59810.1"
                     /translation="MKFLKSFFTTLSVLFVLLLSHFLTYAAEYDFVLTNNTEVAYTTG
                     NDYTDVTINLTREVKDKKYYFSTQGSHTFFIPDLVFDNEEDIKREREFKKESLTVKND
                     KGQDIPYTIEELEYGEGMNIKVPNYRDTTYAVPYRIKLTFKTHDYVKNMYDWVLIEYP
                     MLSKDTKFEIKDES"
     gene            576..1700
                     /gene="ychF"
                     /locus_tag="PK432_02415"
     CDS             576..1700
                     /gene="ychF"
                     /locus_tag="PK432_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOK59811.1"
                     /translation="MENVTRSHSLSIGIVGLPNCGKSTLFNALTKNSVPAENYPFCTI
                     DKNIGVVQIPDERLDRLSNFFKAQKVVPSAMTFVDIAGLVKGASKGEGLGNQFLSHIR
                     EVDAIIYVLRAFKGDSVVHVYNRIDPVDDFEIVQSELIIKDIESVEKRYESVKRNSKS
                     GITESIAMEFSVLEKILKGLNEGKPVIDMVLKKEEREIVHDLFLLTDKPRIFILNVKE
                     GMDDENLEEWKKKLAEISGDTENKYIIEADVKLLSDLADMKEEEFNEYVQLLGKKPVT
                     VEDIIKKAYERLNLITFYTGSSKECNAWSIQKGADIKSAAGVIHTDFEKNFITADVVN
                     VDDLINAGGWGVAKEKGLVKNHGKEYIVNDGDYVVILANA"
BASE COUNT          671 a          222 c          398 g          651 t
ORIGIN      
        1 tgattcatcc tttatttcaa attttgtgtc tttacttagc attgggtatt caattaatac
       61 ccagtcatac atatttttga cataatcatg tgttttaaat gtgagtttta tcctgtatgg
      121 aacagcatat gtagtatctc tataatttgg tacttttata ttcattcctt ctccatattc
      181 tagctcctct attgtgtagg gaatgtcttg tcctttatca tttttaactg taagagactc
      241 tttcttaaat tctctctccc tctttatatc ttcttcatta tcaaatacaa ggtctggtat
      301 aaaaaaagta tgactacctt gtgtagaaaa gtaatacttt ttgtctttta cttctcttgt
      361 taaattaatt gtcacatctg tatagtcatt cccagttgtg tatgcaactt ctgtattgtt
      421 agtaaggaca aaatcatatt ctgctgcata tgttaaaaaa tgagaaagaa gtaacacgaa
      481 cagaacagag agtgtagtaa aaaaagattt taaaaatttc atatacaaga tatattactt
      541 tagaattgga agaaaagaaa gtgatataat aaagaatgga aaatgttaca aggtcacact
      601 ctttatcaat aggaatagtt ggtcttccta actgtgggaa gtctacactt tttaatgcat
      661 tgacaaaaaa tagtgttcct gctgaaaact accctttttg tacaattgat aaaaatattg
      721 gtgtagtaca aattcctgat gagagattgg acaggttaag caattttttt aaagcacaaa
      781 aggttgtgcc ttctgcaatg acatttgttg atattgcagg acttgttaaa ggtgcatcaa
      841 agggagaagg tcttggtaat cagtttcttt cacatattag agaggtagat gcaataatat
      901 atgttcttag ggcatttaag ggggactcag ttgttcatgt atataataga atagatcctg
      961 ttgatgattt tgagatagta cagtcagaat taataattaa agatatagag agtgtagaaa
     1021 aaaggtatga gagtgtaaaa agaaattcta aatcagggat tacagaaagt attgctatgg
     1081 aattttcagt tttagagaag attcttaaag ggcttaatga gggtaaacct gttatagaca
     1141 tggttcttaa gaaagaggag agagagattg ttcatgattt gtttctgctt acagataaac
     1201 caaggatatt tattcttaat gtaaaagaag ggatggatga tgagaatctt gaagagtgga
     1261 agaaaaagct tgcggagata tctggtgata cagagaataa atatataatt gaggcagatg
     1321 ttaagcttct ttctgatttg gctgatatga aggaagaaga gtttaatgaa tatgtacagc
     1381 ttttgggtaa aaaacctgta actgttgaag atatcattaa aaaagcatat gagagactta
     1441 acctaataac cttttatact ggttcttcaa aagagtgcaa tgcatggagt atacaaaagg
     1501 gggcagatat taagagtgca gcaggtgtaa ttcataccga ttttgaaaag aattttataa
     1561 cagctgatgt tgtaaatgta gatgacctta ttaatgcagg gggatggggt gtagctaaag
     1621 aaaaagggct ggttaaaaat catgggaagg agtatattgt taatgatggg gattatgtag
     1681 tgatacttgc taatgcataa gtgttgatat atacactttt ttctgctaaa atccacttgc
     1741 ttgttataaa aatatattaa ataaaagggt atataaatga agaaagacaa aggaaaaatg
     1801 agaacttatg agcttatgat tgcattaaag cctcttttgc ctgatgattt aaggaaagct
     1861 attcataaag agcttgttga tatgatgagt aaggatggag gagaagtttt agatgttgat
     1921 gtatggggta aaaggtatct gg
//
LOCUS       DAOSAB010000116         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_40019_length_1929_cov_3.068303, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000116 DAOSAB010000000
VERSION     DAOSAB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40019_length_1929_cov_3.068303"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PK432_02420"
     CDS             <1..1111
                     /locus_tag="PK432_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOK59812.1"
                     /translation="FKEKDIKRKAKVEKLLNIDIDKGKYDHYMLKEIVQQQFTILKAT
                     SYSIKDLKPLIERIRNSKNIYTIGAGTAGYAGDQVSYYLRKISKRNAISIKAYEIESY
                     VDIVGKDDVVIAFSQSGETADTLEAVETFKKNGAYICSVINMPGSTLSRVSDIFFMSN
                     AGSEICVASTKVFTSQISFGYLLAKSLINEYSEAKKELSLLSQNLETFFNEKTFSNIK
                     KISKLLKNKEHFFILGKGQNYHIAQEGALKIKEISYKHFEGFAAGELKHGVIALIEKG
                     TPVISIISDDTNYKDMISATYEVKARGALTIGVGDGKFAKENSFDYFIPVAQSNELKA
                     VANVIPFQLISYYLALSLGNNIDKPRNLAKSVTVK"
     gene            complement(1112..1828)
                     /locus_tag="PK432_02425"
     CDS             complement(1112..1828)
                     /locus_tag="PK432_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59813.1"
                     /translation="MSIFDFFTGGSRISTTSFEQLRSKIVKLREQNLYPYTFNLPQAI
                     SFPLDFWDELIKIYKKTNSDGLERAFSVFWADGEIVLTEVKTGSDRMVKSGGSIQVKY
                     SQHPTKKGYARKELLVDEKVLKRRDVYYKNIPKTLTVQYLFNIHTHPKHIDENGNVYY
                     NFFSAQDIKSWISSKAIMTGVVTDGLWILIRSDKTRKDIENLVDNQITPQFLEDDLYI
                     GVYRAEFNKKAYRYKLISNG"
BASE COUNT          687 a          321 c          292 g          629 t
ORIGIN      
        1 ttttaaggaa aaagatataa aaagaaaagc taaagttgaa aaactcctta atatagatat
       61 tgataaaggg aaatatgacc actatatgct taaagaaata gtccagcagc agtttacaat
      121 actaaaagca acttcatatt ccataaaaga tttaaaacca ttaatagaaa ggattagaaa
      181 ctcgaaaaat atatatacaa taggagcagg aacagcagga tatgctggtg atcaggtttc
      241 ttactaccta agaaaaatat caaaaagaaa tgctattagt attaaagcgt atgaaataga
      301 atcatatgtt gatattgtag gaaaagatga tgtagtaatt gctttctcac aaagcggtga
      361 aactgctgat acacttgagg ctgttgagac atttaaaaag aatggagcat acatctgtag
      421 tgttattaat atgcctggtt caactctttc cagagtatca gatatctttt ttatgtctaa
      481 tgctggaagt gaaatatgtg tagcttctac aaaagtgttt acatctcaga tatcttttgg
      541 atatttactt gcaaaatcac taattaatga atactcagaa gcaaagaaag agctttcact
      601 tctttcacag aatttagaga ctttctttaa tgaaaaaaca ttcagcaata ttaaaaaaat
      661 atcaaaacta ttaaaaaaca aagaacattt ctttatcctt ggaaaaggac agaattatca
      721 tatagcacag gaaggtgcac ttaagataaa agaaatatca tataaacact ttgaaggatt
      781 tgcagctggt gagcttaaac atggtgtaat tgcattaatt gaaaaaggta cacctgttat
      841 ttctattatt tcagatgata caaattacaa agatatgata tctgcaacat atgaagtaaa
      901 agcaagagga gcattaacaa ttggagtagg ggatggtaaa tttgctaaag aaaactcttt
      961 tgattacttt attcctgttg cacaaagcaa tgagctaaaa gctgttgcga atgtaattcc
     1021 tttccagtta atatcctatt atttagcact ttctcttgga aataatatag ataaaccaag
     1081 aaatcttgca aaatcagtta cagtaaaata actatccgtt agatatgagt ttgtatctat
     1141 atgccttttt gttaaattct gctctgtata caccaatata caagtcatct tctagaaatt
     1201 gtggtgtaat ttgattgtca acaagatttt caatatcctt tcttgtttta tcactcctta
     1261 tcagtatcca tagtccatca gttacaactc ctgtcataat tgcttttgat gatatccatg
     1321 acttaatatc ctgagctgag aagaagttat aatatacatt gccattttca tctatatgtt
     1381 ttgggtgagt gtgaatatta aacagatatt gaactgttaa tgttttaggt atgtttttgt
     1441 aatatacatc tctccttttt aataccttct catctactaa aagctctttt cttgcatatc
     1501 ctttcttagt agggtgctgt gagtatttaa cttgaattga tccaccgctt ttaaccattc
     1561 tatcagaacc cgttttaact tctgtcagta caatctctcc atccgcccaa aatactgaga
     1621 atgctctttc aagcccatca ctatttgtct ttttgtatat ctttattaac tcatcccaga
     1681 aatctaaagg aaaggatata gcctgaggaa gattaaaggt atatgggtat aaattctgtt
     1741 ctcttaattt tacaatctta ctcctaagct gctcaaagga agtagtagaa attctagatc
     1801 cacctgtaaa gaaatcaaat atactcattt tttaattgac catattattt accatgcacc
     1861 accagaagag cctccaccac ctcctccaga agaaccacct ccaccaaaac cagaacctcc
     1921 tgaactcca
//
LOCUS       DAOSAB010000117         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_40452_length_1915_cov_2.529032, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000117 DAOSAB010000000
VERSION     DAOSAB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40452_length_1915_cov_2.529032"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..548)
                     /locus_tag="PK432_02430"
     CDS             complement(126..548)
                     /locus_tag="PK432_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK59814.1"
                     /translation="MVKTVEDKQRFVELRAKGLSFERIAEELKVSKPTLIKWSEELFH
                     EVQEAQFYELENLLENYKLMRKQRFELYCKLLSSALAELESRAAAGKLQEVPTEKLLS
                     MAEQLEKRVQQDTQQELVSVTLGIGDFKIGTEKFLEAG"
     gene            complement(555..1598)
                     /locus_tag="PK432_02435"
     CDS             complement(555..1598)
                     /locus_tag="PK432_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59815.1"
                     /translation="MLDTLKLMLSEYKVSPESSLRVQPASYEVATGEKVEYPLFRNEA
                     GTFYGSKAYLNTENWNLTLKPLPAGSRGVGAFLQFSVPKNYYGSNYFSVGEAGTRAAL
                     KKVENELVQNGVFTNLEEAELSRVDTFKNIEPEEPFSSYFSLFSLLKARKAHQRGYGT
                     TFLVANSSQEFCVYDKLQEMREHGLETSELPETMRFEHRALKKEKVRSLYGFTRVNEL
                     FHGGYEVVKEKQVESWKNSLFSFSAEEVVVLGSKQLEQEMKAFQEKFGSNWFQYFLKS
                     YGAYSLAKFAGKEVVRAALENLNSERTKVWRTMKLLEEAERELLVLKQEEGSSKTLGE
                     LYEELREKVCELE"
     gene            1625..>1915
                     /locus_tag="PK432_02440"
     CDS             1625..>1915
                     /locus_tag="PK432_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59816.1"
                     /translation="MQQTPIVIHGSLLFLQTGALKTLKRLYNSYTRTEKRLRSSSSKI
                     SFHLTTSHLLSSFRAENQNREEVEELQQPKFLFMVQHLLSLLLMPHLACLPKP"
BASE COUNT          525 a          509 c          311 g          570 t
ORIGIN      
        1 cattgccttc cagagcaaac tcaccagttc tcatttctca cctcactttc agagaacctg
       61 ctcttttacc tcttagcagg caaaatacag gtaaaaaaac agtaaaaaaa cagtaaaaga
      121 ccatatcagc ctgcttccag aaacttttca gttcctatct taaaatctcc tatacctaaa
      181 gtaactgaaa ccagttcctg ctgagtatcc tgctgaactc tcttctccag ttgctcagcc
      241 atgctcagca acttttcagt tggaacttcc tgcaactttc cagctgcagc tctgctctcc
      301 agctcagcaa gtgcagagct caggagcttg cagtaaagct caaacctctg ctttctcatg
      361 agcttgtaat tctccagcag gttctccagc tcatagaact gagcttcctg aacctcatga
      421 aagagctcct cactccactt aatcagagta ggctttgaaa ccttgagttc ctcagctatt
      481 ctctcaaagc tcaaaccctt tgctctgagc tcaacaaacc tctgcttatc ttcaactgtt
      541 ttcaccatat cacctcactc aagctcacac actttttctc taagttcctc atagagttct
      601 cctaaggttt tactggaacc ttcttcttgt ttcaaaacta aaagctctct ctcagcctcc
      661 tctaagagct tcattgttct ccaaactttt gttctctctg agttcaggtt ctccagagct
      721 gctctcacaa cttcttttcc agcaaacttt gctaaggagt aggctccata gcttttcaga
      781 aaatactgga accagtttga accaaacttc tcctgaaaag ccttcatttc ctgctcaagc
      841 tgctttgagc ctaacaccac cacttcctca gctgagaaag aaaacaggct atttttccag
      901 ctctcaactt gcttttcttt caccacctca taaccaccat gaaaaagctc atttactctt
      961 gtaaaaccat agagagagcg cactttttct tttttcagtg ctctatgctc aaacctcatt
     1021 gtttcaggaa gctcagaagt ttccaaacca tgctctctca tttcctgcaa cttatcatat
     1081 acacagaact cctgagagga gttagcaacc agaaaagtgg tgccatagcc tctctggtga
     1141 gcctttcttg cctttaacag tgagaaaagt gaaaaataac tggagaaagg ttcctcaggt
     1201 tctatgttct tgaaggtatc tactctggag agctctgctt cttctaagtt ggtaaaaact
     1261 ccattctgaa ccagctcatt ctcaaccttc ttcagagctg ctctggttcc agcctctcca
     1321 actgaaaaat aattggaacc ataatagttc ttaggaactg aaaactgcag aaaagctccc
     1381 acccctctac ttccagcagg aagtggtttg agtgttaggt tccagttctc agtgttcagg
     1441 tatgcttttg aaccatagaa ggttccagcc tcatttctga aaagtggata ctcaaccttt
     1501 tctccagttg caacctcata gcttgcaggt tgaactctca gtgaactctc tggagaaacc
     1561 ttatactcag agagcatgag cttgagagtg tctaacatgc taaaaccccc tgttgttgca
     1621 aagtttgcaa caaactccta ttgttataca tggaagtttg ttgtttctgc aaactggagc
     1681 tctcaaaact ctgaaaaggt tgtataatag ctataccaga acagagaaga ggttgaggag
     1741 ctccagctcc aaaatctctt ttcatttaac cacctcacac ctgctcagct ctttcagagc
     1801 tgagaaccag aacagagaag aggttgaaga actccagcaa ccaaaatttc ttttcatggt
     1861 tcagcacctt ctctctcttc ttctaatgcc tcacttagct tgccttccaa agcct
//
LOCUS       DAOSAB010000118         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_40609_length_1910_cov_2.283019, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000118 DAOSAB010000000
VERSION     DAOSAB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40609_length_1910_cov_2.283019"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PK432_02445"
     CDS             complement(<1..311)
                     /locus_tag="PK432_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59817.1"
                     /translation="MEEKVSDSTKNNRSLPDERYDIRLYKEAVKASKEAKRIAEKERR
                     KKRVEKTFLGIILFIVLCFGIYLISKVYAPKPEYIEAHEYPLSLSYESIGEENISQPA
                     N"
     gene            413..1750
                     /gene="aspS"
                     /locus_tag="PK432_02450"
     CDS             413..1750
                     /gene="aspS"
                     /locus_tag="PK432_02450"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOK59818.1"
                     /translation="MERTYISQLKDLIGKSVRIRGRVDTIRDQGKIAFLIVRDITGKV
                     QSVAIQNENNSILKTVKELTNESVVDIIGDVKEAKQVATGYEVEIKEIKVDSLSQTPL
                     PIVIEQPYSKNISNLDKRLDYRWIDLRSERNNLMMRVATQMGKALREFCLNNHFIEIQ
                     APRIISAASEGGANVFEVKYFDRKAYLAQSPQFHKQMGIAADLDRVFNMGPVFRAEKS
                     FTTRHLTEYTSFDVEIGYIDSYREVCQFEQEMIKYTLQSIKETLGEEIKRVFDIDIKV
                     PQGDFAYISVKEIKEKLAKEGIPSKEEGDFSPEEERAISQIMERETGQEFAFVYDYPH
                     KNRAFYHMKDPNRQEFALGFDLFWKGIEITTGAQREHRLENLKENAKERGISLDSLAD
                     YFEYFEYGCPPHGGFALGTERFLMKLLNYSSILETTYLPNTPNRVGKLLNKKK"
BASE COUNT          734 a          292 c          330 g          554 t
ORIGIN      
        1 aaatttgcag gctgactaat attttcctcc cctatagact cataacttaa actcaatgga
       61 tattcatgag cttcaatata ctctggcttg ggagcataaa ctttacttat cagatatata
      121 ccaaagcaaa gaacaataaa aagaataata cctaaaaaag ttttttctac tcttttcttt
      181 ctcctttctt tctctgctat tcttttagcc tcttttgaag ccttaactgc ttctttgtaa
      241 agcctaatat catatctttc atcaggtagg gatctattat tttttgtact atcagaaact
      301 ttttcttcca ttaacagtat tgatattatc agctaaatag ccttaaaaca aatattttgt
      361 tttttgtatg gaatgtaata tataatagca aagtaatata acagaaaaaa agatggaaag
      421 aacatatata tcacagctaa aagatttaat tggaaaaagt gtaaggataa ggggaagagt
      481 cgatactatc agagatcaag ggaaaatagc atttctaata gtaagggata taacaggaaa
      541 ggtacaaagt gttgctatac aaaatgagaa caatagtatt cttaaaacag ttaaagaact
      601 aactaacgag tcagtagttg atattattgg tgatgttaag gaagcaaaac aggttgcaac
      661 tggatatgag gttgagataa aggagataaa ggtagattca ctttcacaaa ccccgcttcc
      721 aattgtaatt gaacaaccgt attcaaagaa tatatcaaac ttagataaaa gacttgatta
      781 cagatggatt gatctgcgct ctgaaagaaa caacttaatg atgagagttg ctactcagat
      841 gggaaaagca ttaagagagt tttgtctaaa taaccacttt attgaaatac aggctcctag
      901 aataataagt gctgcaagcg aaggaggtgc taatgtgttt gaggtaaaat actttgatag
      961 aaaggcatac cttgcacaat cacctcagtt tcataaacaa atgggaatag cagcagatct
     1021 agacagagta tttaatatgg gtcctgtatt tagagcagag aaatcattca caacaagaca
     1081 cctaacagag tatacaagtt ttgatgtaga aatcgggtat attgattcat acagagaagt
     1141 ctgtcagttt gaacaagaaa tgattaaata cacacttcaa agcataaaag aaacattggg
     1201 agaagaaata aaaagagtat ttgatattga cattaaggtc ccacaagggg attttgcata
     1261 tatctcagta aaggaaatta aagaaaagct cgcaaaagaa ggtattccaa gcaaagaaga
     1321 aggagatttc tcacctgagg aggaaagagc aatatcacaa ataatggaaa gagaaacagg
     1381 gcaggagttt gcatttgtct atgactatcc tcataaaaac agagcctttt atcatatgaa
     1441 agatcctaat agacaagaat ttgctttagg ttttgatctg ttttggaaag gtattgaaat
     1501 aacaactggt gctcaaagag aacataggct tgaaaatcta aaagagaatg caaaggaaag
     1561 aggtatatca cttgattctc ttgcagacta ttttgaatat tttgaatatg gatgtcctcc
     1621 acatggagga tttgcattag gaacagaaag attcttaatg aaacttctta attacagcag
     1681 tattcttgaa acaacatatc ttccaaatac tcccaacaga gtaggaaagt tgttaaacaa
     1741 aaagaaataa ctaagaagaa aatctccact ctttaacaag aaattgaaca tcttcatgac
     1801 cattccaaga gtttattcct acatctccta caacatcaat aatatcattt acatctatct
     1861 cctcactgtc cccattacag ttaaaaaaca caagtgttat taaatctata
//
LOCUS       DAOSAB010000119         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_41093_length_1893_cov_1.803047, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000119 DAOSAB010000000
VERSION     DAOSAB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41093_length_1893_cov_1.803047"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PK432_02455"
     CDS             <1..260
                     /locus_tag="PK432_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59819.1"
                     /translation="TNNTVVIHYQRPFEDLDADVDEPDFPQEWFDALCYGLACRLAPS
                     YGIPLADRKQLWNEMTIIKQDAMNFGLEEGSMFFQRDFRNW"
     gene            268..>1893
                     /locus_tag="PK432_02460"
     CDS             268..>1893
                     /locus_tag="PK432_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59820.1"
                     /translation="MQPDSSQILGLDQIADAVNRAKLGSQRRKNEMIRLGTTDPTSFQ
                     MNFKNQLKEQQGQQDSLFAGSKLAGNQSPDQETVDYVGNALSKAGGKYYYYGDFDYSD
                     PNNVTNALKSKGWKSSKSTVDPTLYNLLSLKNPVTSYKGQHYYQGDVDPSSAQWGTAG
                     YNMQDLGGGTYNILDAGGTSIGKGYKGLDDTIRELAAQQEQSKNPYESSMPINVGEMD
                     NYTPSFPNPTQYTNSSPYSAGDLDKWEVLGQVLSGQPISSDPTTNRSSMAMSGDNQNQ
                     SIKGLQSLFGSTPLIYNNKVMGYKMDPTPASKDMLGYVDPYTVRREDTKGSTQSSYGL
                     QRQYNDPNTWNQLTKSLDANSLYAPAENAEKLPGWTNVDNSQYHHQSSGLGNKLMQGL
                     GSILSFTPLAPLGLAMNTLGSLASGNHLGGILGAVTGGLGQAGAFDKLGGSVGDALGT
                     SGKIGSSIVRGGVGALSGLAQGNNVKDALLGGIGSGLGPLAGDYISSNLNGTLGKTGS
                     NMIGSGASGALQSLFKKQNIGEGALSSSLASGLG"
BASE COUNT          618 a          288 c          394 g          593 t
ORIGIN      
        1 caactaataa tacagttgtt attcattacc aaagaccatt tgaggatctt gatgcagatg
       61 ttgatgaacc tgactttccc caagaatggt ttgatgctct ctgttatggt cttgcttgta
      121 ggttagctcc ttcttatggt attccactcg cagatagaaa acaactctgg aatgaaatga
      181 ctattatcaa acaggatgca atgaactttg gtttagagga aggttctatg ttcttccagc
      241 gtgattttcg caattggtga ttaaataatg caaccagatt cttctcaaat tttaggttta
      301 gatcaaattg ctgatgcagt taatagagct aaactaggaa gtcaacgacg aaaaaatgaa
      361 atgataagat tgggtacaac tgatccaaca tcatttcaaa tgaattttaa aaatcaacta
      421 aaggagcagc aaggacaaca ggattcttta tttgctggta gtaaactagc aggtaatcaa
      481 agtccagatc aagagactgt agattatgta ggcaatgctt tatctaaagc tggtggtaaa
      541 tattattact atggtgattt tgattattct gatcctaaca atgttactaa tgctttaaaa
      601 agtaaaggct ggaaatcatc taaaagtact gtagatccta cattatataa tctattatct
      661 ctaaaaaatc ctgttacctc ttacaaaggt caacattatt atcaaggtga tgtagatcct
      721 agtagtgccc aatggggtac tgcaggatat aatatgcaag atctaggtgg tggtacttat
      781 aatattttag atgctggtgg tacttctata ggtaagggtt ataaaggttt agatgatact
      841 ataagagagt tagctgctca acaagaacaa agtaaaaatc catatgaatc aagtatgcct
      901 attaatgttg gggaaatgga taactataca ccatcttttc ctaacccaac acaatatact
      961 aatagttctc cttactctgc tggtgattta gataaatggg aagtactagg acaagtactt
     1021 agtggtcaac caatttcaag tgatccaaca acaaatagat catctatggc aatgtcgggt
     1081 gacaatcaaa atcaaagtat taaaggatta caaagtctat ttggatcaac accattaatt
     1141 tataataata aagttatggg ttataagatg gacccaacac cagcttcaaa ggatatgtta
     1201 ggttatgttg atccatatac tgtccgaaga gaagatacaa agggtagcac tcaatctagc
     1261 tatggtttac agagacaata taatgatcct aatacatgga atcaattaac taaatcacta
     1321 gatgctaata gtctttatgc tccagctgag aatgctgaga aacttcctgg atggactaat
     1381 gtagataact cacaatacca tcatcaaagt agtggtttgg gtaataaatt aatgcaaggt
     1441 ttaggatcta ttctgagttt cactccttta gcccctcttg gtttggctat gaatacttta
     1501 ggttctttag cttctggtaa tcatcttggt ggtatattag gggcagtaac tggaggattg
     1561 ggacaagctg gagcttttga taaactaggc ggttctgtag gtgatgcact aggtactagt
     1621 ggtaaaatag gttcatctat agtgcgtggt ggtgttggtg ctttatcagg actagcacaa
     1681 ggtaataatg ttaaagatgc cttactaggt ggaattggtt ctggattagg accattagca
     1741 ggagattata tatcttcaaa tctaaatggt actttaggaa agactggttc taatatgatt
     1801 ggtagtggtg ctagtggtgc tctacagagt ctgtttaaga aacagaatat aggtgaggga
     1861 gcacttagta gtagcttagc ttctggtctt ggt
//
LOCUS       DAOSAB010000120         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_41513_length_1880_cov_2.813151, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000120 DAOSAB010000000
VERSION     DAOSAB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41513_length_1880_cov_2.813151"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PK432_02465"
     CDS             complement(<1..296)
                     /locus_tag="PK432_02465"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOK59821.1"
                     /translation="MEKNGYNRKLFSLISILASLFVYTGAIAIYLYANGWRLGNSNQL
                     LVKTGVLTVQSSPSLADLYVNGEKEGRTSKSVSLPVGIHSVLVKKNGYVEWKKDV"
     gene            350..1366
                     /locus_tag="PK432_02470"
     CDS             350..1366
                     /locus_tag="PK432_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOK59822.1"
                     /translation="MLGKKLGIDLGTANSVVFVQGEGIAFVEPTVVAIDVNTFKVIAV
                     GSEAKKMIGKTPENIIAKRPLKNGVIADSRITEALLKYFFNKALGKSRFFKPDVVISV
                     PAGITTVEQRAVIKAANNAGAREITMLPEPLLAALGAGLPIETSSGNMIVNLGGGTAE
                     VAVISLDGIVEYDSLRIAGDAINEAIINYMKKKKGILIGEQTAENIKIRVGSAIEIKD
                     PKSMEVRGRDFGAGLPKAITVDSNDVAAAVKRPLLSIINSIKGVLERTPPELSADIMD
                     RGMAMTGGTSKLRNLDKLFIKAIGVPAYVADEPMYCVAKGTGIALELMAEGERKFSFN
                     RAVT"
     gene            1367..>1880
                     /locus_tag="PK432_02475"
     CDS             1367..>1880
                     /locus_tag="PK432_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59823.1"
                     /translation="MSIFSKTGKQEMKLEEVVSLMSKLRLPISLVFTPVNLREEKEKF
                     LNSDVYEPVFKYRRVRNNNEEILKRLSSLKLITDVDPRISDFYIQLIASKNDSNILMN
                     AVGNNELVTEVSYRKYGVPTPLLFRNASRILRGNTSGYNIIKPPVIKEGDIFHYEKIK
                     EIFNLVFEHFG"
BASE COUNT          653 a          261 c          380 g          586 t
ORIGIN      
        1 acatcctttt tccattcaac atacccattt ttcttaacta acacactgtg tattcctact
       61 ggtagactta cactttttga agttctacct tctttctctc cgttaacata aagatctgcg
      121 agagaaggag atgattgtac agttaagacg cctgttttta ctaacagctg attagagttg
      181 ccaagcctcc atccgtttgc atacaaatat attgctattg ccccagtata tacaaataat
      241 gaggctagta ttgatattaa agaaaacagc tttctattgt aaccattctt ctccatgaca
      301 aatttagtat atcatatcta gaacttaatt ttattgatat aattaggtta tgttaggaaa
      361 gaaactagga atagatctag gaacagctaa ttctgtagtg tttgtacaag gcgaaggtat
      421 tgcctttgtt gagcctactg ttgtggcaat tgatgtaaat acatttaagg taattgctgt
      481 tggatctgaa gctaaaaaaa tgattggtaa aacacctgag aatattattg caaagaggcc
      541 tttaaaaaat ggagttattg ctgattcaag aatcacagaa gcactgctta aatatttttt
      601 taataaagca ttaggaaagt ctaggttctt taagcctgat gttgtaatta gtgttcctgc
      661 agggattaca acagttgagc agagagctgt aataaaggct gcaaataatg ctggtgcaag
      721 agaaattaca atgcttcctg agcctctttt agctgcatta ggtgcaggtt tacctattga
      781 gacctcatcg ggcaacatga ttgtaaattt aggtggcggt acagcagagg ttgcagtaat
      841 atctcttgat ggaatagtgg agtatgactc tttaagaatt gcaggagatg caataaatga
      901 agcaataatt aactatatga aaaagaaaaa aggtattttg ataggtgagc aaacagcaga
      961 aaatattaaa ataagggtag ggtctgcaat agaaataaaa gatccaaaaa gtatggaggt
     1021 aagggggaga gattttggtg caggtttacc taaggcaatt accgtggact ctaatgatgt
     1081 agcagctgct gtaaaaaggc ctttgctaag tattattaat agtattaaag gagtgttaga
     1141 aaggacacct ccagagctgt ctgctgatat tatggataga ggaatggcaa tgactggtgg
     1201 aacatcaaaa cttaggaatt tagataagtt gtttataaaa gcaattggtg ttccagcata
     1261 tgttgcagat gagcctatgt attgtgttgc aaaaggaaca ggtattgcat tagagttaat
     1321 ggcagaaggg gaaagaaaat ttagttttaa tagagcagtt acttaaatga gcattttttc
     1381 taaaacagga aaacaagaga tgaaattaga agaggttgtt tccttaatgt caaaattaag
     1441 actccctata tctcttgtct ttacacctgt taatttaaga gaagagaaag aaaaattttt
     1501 aaactctgat gtatatgaac ctgtatttaa gtataggagg gtaagaaata acaatgagga
     1561 aatattaaaa aggctttctt ctttaaagct gattactgat gtagatccaa gaatttccga
     1621 cttctatatt caattaattg cttcaaaaaa cgattctaat atccttatga acgcagtagg
     1681 gaataacgaa cttgtaacag aagtatcata tagaaagtat ggagtaccga ccccacttct
     1741 ttttaggaat gcatcaagaa ttttaagagg aaataccagt ggttataata taataaagcc
     1801 ccctgttatt aaagaaggcg acatttttca ctatgaaaaa ataaaagaga tttttaatct
     1861 tgtttttgag cattttggtt
//
LOCUS       DAOSAB010000121         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_42592_length_1846_cov_2.663317, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000121 DAOSAB010000000
VERSION     DAOSAB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42592_length_1846_cov_2.663317"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="PK432_02480"
     CDS             complement(<1..1664)
                     /locus_tag="PK432_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59824.1"
                     /translation="MLKGIKISKIAFIISFCFLFFFGLVTNIEAYCYCSGSSKVCYSG
                     CDAYGNIKTCTTGCPGGLYCYDTNGTSWGVYCCDYKTTTNCSCGCSGGSCNTCCTPST
                     SCNCSPLSSSSTSYGSKTVTCSDGCGGTASRTCWCTGNCTPQSCPSGTSESGTHGIYA
                     TYTCTNECNKSNSRTCKCTSACTPASCPSGTSESGTHGTYGTYSCINACNESNSRTCK
                     CTSACTPTSCPTGTTETNTGDSYSSYSCTNACSESKTRNCYCSICTPPSCLSRGYSDT
                     NLGYGQVPLEKTPSCRNGQGAPDQLSKCDMKYRTCYCPSCLKQCPSPLANTGSPNLIL
                     NDFRECTNDCSVKPPEDQDDCYEVPSEQPTESLIINGEEADPNGFGFLSLTHTGDNKN
                     IPDQGDLNDPLSPIKMVATYTDTDGADDIEGMFVWFRGEQYTGELGTPIYISDTAEPK
                     APANDSWGFMLRKNGTSDWDPYVPSYQEVQPSWTKAIYTTLLGYRVFYISGPNAKQMV
                     EVTILQQPQKEGDKVTMEFSLRFSNSEGVLYNDPVDEGIYNIYLMG"
BASE COUNT          581 a          389 c          245 g          631 t
ORIGIN      
        1 aaacccatta aatatatatt atatatacct tcatcaacag gatcattata aagtacaccc
       61 tcactattag agaatcttaa actaaattcc atagttactt tatctccctc tttttgaggc
      121 tgttgaagaa ttgttacttc taccatttgt tttgcattag gaccggatat ataaaatacc
      181 ctatatccaa gcaaagtagt atatatagct ttagtccaag atggctgaac ttcttgataa
      241 ctaggaacat atggatccca atctgatgtt ccattttttc ttaacataaa tccccaacta
      301 tcatttgctg gtgctttagg ctccgctgta tctgaaatgt atattggtgt acctaactct
      361 cctgtatatt gttcacctct aaaccataca aacatccctt ctatatcatc tgctccatct
      421 gtatcggtat atgtagctac cattttaatt ggagataatg ggtcatttaa atcaccttga
      481 tcgggtatat tcttgttatc tcctgtatgt gttagactta aaaatccaaa accattagga
      541 tctgcttctt ctccatttat aattaaactt tctgtcggtt gctctgatgg aacttcataa
      601 caatcatcct gatcctctgg tggtttaaca ctacaatcat ttgtacattc cctaaaatca
      661 tttaatatta aattaggact ccctgtattg gctaatggtg aaggacattg ctttaaacag
      721 cttggacagt aacatgtacg gtatttcata tcacacttcg ataactgatc tggtgctccc
      781 tgtccatttc gacatgatgg tgttttttca agaggaactt gcccatatcc taaatttgta
      841 tcactatatc ctctgcttaa acaagaagga ggtgtacaaa tactacaata acaattccta
      901 gttttagatt cactacaagc atttgtacaa ctatatgaag aataactatc tcctgtattt
      961 gtctctgttg ttccagttgg acagctagtt ggtgtacatg cacttgtaca tttacatgtt
     1021 ctactattac tttcattaca tgcatttata caactatatg tcccatatgt accatgtgtt
     1081 ccactctctg atgttcctga tggacaactg gctggtgtac atgcacttgt acatttacat
     1141 gttctactat tacttttatt acactcattt gtacaagtat atgtagcata tataccatgt
     1201 gtcccagact ccgatgtacc cgatggacaa ctttgaggag tacaattccc agtacaccaa
     1261 caagttctac ttgcagtacc accacaacca tctgaacaag ttactgtttt tgagccatat
     1321 gaagttgaac tactagataa tggagaacaa ttacaactag tacttggtgt acaacatgtg
     1381 ttacatgatc caccactaca accacaacta cagttggtag ttgttttata atcacaacaa
     1441 tagacccccc atgaagtacc attcgtatca taacaataaa gtccaccagg acatccagta
     1501 gtacatgtct taatattccc atatgcatca caacctgaat agcaaacctt actacttcca
     1561 ctacaataac aatatgcttc tatattagtt actaatccaa agaaaaacaa aaaacaaaaa
     1621 gaaataatga aagctatctt tgatatcttt attcctttta acatttaagc attcatacta
     1681 aattaagcca cttcttctat taaaatatac tatggtttaa cacctaattc tactgtttgt
     1741 attatatatt tactcccaaa gaactcctct cctaatacac catatttagg atgcaaatct
     1801 atagctaata atggaaattt taatctgtct tctaatctta aatacc
//
LOCUS       DAOSAB010000122         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_42983_length_1834_cov_2.179876, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000122 DAOSAB010000000
VERSION     DAOSAB010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42983_length_1834_cov_2.179876"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..782)
                     /locus_tag="PK432_02485"
     CDS             complement(150..782)
                     /locus_tag="PK432_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59825.1"
                     /translation="MGNQELTTANNEELKTFLSTLIESKKLPSHIKTVEDAFTIAQMG
                     KELGFPTMQAFHYIIPIQGKLSLSAKATGALLRKGGVKFYTKEDGVYVYKDGSTSDIR
                     KDIAENPMDRRTTIVFMRDGVEEACTFTWQDATGQGLNTKDNWKRMPKEMLYARCLAK
                     GANRIGADLLLGLYMTEELTDSFNVPETAVKRNEDGTIKEIIDITHTEVK"
     gene            complement(782..1339)
                     /locus_tag="PK432_02490"
     CDS             complement(782..1339)
                     /locus_tag="PK432_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59826.1"
                     /translation="MSIPTVSSPEDKKENLKKIKEFHKATLANLGVSDYSLIPKLAYR
                     PSGKTELFVSFFYSEISKGQDVYLEFTDRNNVPEDPERTLYLWKFNPHFEEEYEKTEP
                     TEGTGHVRYLVPVEELKVIKKYSPEASITTEKEIKLKPALDFSLPNPETDPPINDMTI
                     RDLAAIILGKPVSNKEWLNQIIKNK"
     gene            complement(1336..>1834)
                     /locus_tag="PK432_02495"
     CDS             complement(1336..>1834)
                     /locus_tag="PK432_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59827.1"
                     /translation="TGKDELVLKYKNTIVTLDHSLLLDNSPYGDDMSMLKALGKTVTA
                     LKRKYPIAFIILSQLNRNIDAPERNEDGKYGNFILDSDIFGSDALLQHADTLIGLNRP
                     GKQKLTFYGVERYIINQDPTILAMHFLKCRNGETGIGFFRTEFEKMNIVEIPTPARQE
                     RRIRT"
BASE COUNT          475 a          370 c          273 g          716 t
ORIGIN      
        1 atgctcctgc cttaaacttg aactcctcta aagttattcc ggttatttta cataaattta
       61 ttccaggttc taatgtcttt gacactccac catctccatc agtcttgatg ttctttgtgc
      121 ttaatttact catataatta ttgtttagat tattttactt ctgtatgggt tatatcaatt
      181 atctctttta ttgtaccatc ttcatttctc tttactgcgg tttctggtac attgaagcta
      241 tcagtcagct cttctgtcat atacaagcct aataacaaat cagcaccaat acggttggca
      301 ccctttgcta aacatctagc atataacatt tccttaggca tgcgcttcca gttatctttg
      361 gtatttaaac cttgacctgt tgcatcttgc caagtgaatg tacatgcttc ttctacacca
      421 tctctcataa agacaatagt ggttcttcta tccataggat tttctgcaat atcttttctg
      481 atatcacttg ttgaaccatc cttgtaaaca tatacaccat cttctttagt atagaatttt
      541 accccacctt ttcttagtaa agccccagtt gcttttgcac ttaagcttaa cttaccttgg
      601 ataggaatga tgtaatgaaa tgcttgcata gtagggaatc ctagttcctt acccatctgc
      661 gcgatagtga atgcatcttc tacagtctta atatgacttg gtagtttttt tgattcaatg
      721 agagttgaaa gaaaagtctt taactcttca ttgtttgctg ttgttaattc ttgattaccc
      781 attacttatt ttttattatt tgatttaacc attctttgtt gctcactggt ttacccaata
      841 tgattgctgc cagatctctg atagtcatat cattgattgg cggatcagtt tcgggattag
      901 gtaagctaaa atccaaagca ggttttaatt taatttcttt ctctgtagtt atactagcct
      961 ctgggctata tttctttatt actttaagct cctctacggg aactaagtat ctcacatgac
     1021 ctgtaccttc tgttggctcg gttttctcat actcttcttc aaaatgtgga ttgaacttcc
     1081 ataaatataa agttctttcc ggatcttcgg gaacattgtt tctgtctgta aattctaggt
     1141 atacatcttg tcctttgctt atttcactat agaaaaagct tacaaacaat tctgttttac
     1201 ctgatggcct gtaggccaat ttagggatta gtgaataatc acttactccc agatttgcta
     1261 gagtagcctt atgaaactct ttaattttct taagattctc tttcttgtct tctggactgc
     1321 ttactgttgg tatactcatg ttcttatcct tctttcttgt cttgctggag ttggtatctc
     1381 cacaatattc atcttctcaa attctgttct gaagaaccct ataccggttt ctccattcct
     1441 acattttaaa aagtgcatgg ccaagattgt aggatcttgg ttaataatat atctctctac
     1501 accatagaat gtaagcttct gcttacctgg tctattaaga cctattaaag tatctgcgtg
     1561 ttgcaatagt gcatctgatc caaatatatc tgaatcaaga ataaagtttc catacttacc
     1621 atcttcattc ctttctgggg catcaatatt tctattgagc tgactaagaa taataaatgc
     1681 tattggatac tttcttttta gtgcggttac tgttttacca agagctttta gcatactcat
     1741 atcatcacca taaggactat tatctaataa taatgagtga tccagagtta ctatagtgtt
     1801 cttgtacttt aatacaagtt catcctttcc ggta
//
LOCUS       DAOSAB010000123         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_43163_length_1828_cov_3.997180, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000123 DAOSAB010000000
VERSION     DAOSAB010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43163_length_1828_cov_3.997180"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PK432_02500"
     CDS             complement(<1..1089)
                     /locus_tag="PK432_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59828.1"
                     /translation="MEDNTIVEKIVVEPSHELTDLVRAIHKSKAERIILTFTEHSDIL
                     ISPINLKVLNETAEREGKLLITQIIQNPTGIRNSLLAGIKAIDSPSNPTADDWQEAAE
                     ILERKKKEQLERKKTLKNISAIKESESEAFEEKVESVIEKASEEVKKTETPKEEGMSQ
                     YVDRRGLKTSSPFISIDQDIPKIEKDIITENIHSEPKKEKPKFFKFKPPFNRNNNIPT
                     AGKKKIPKTFLKIIIPLLALLIIGGILYNHFATFVKVKIFVESKPVKIETVLSGDENV
                     EEIDFENSKIPIKTEEKSKSLSNTITATGKAYKGEKAEGKIKIYFSPECTEEIQPITL
                     EAGHTLTTEIGGKTLSYKLKSDVTVVCPE"
     gene            complement(1091..>1828)
                     /locus_tag="PK432_02505"
     CDS             complement(1091..>1828)
                     /locus_tag="PK432_02505"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HOK59829.1"
                     /translation="NSLVGYKGRNVKLNFYASFAPRTYTEALKKVASSLNFEVLGIVS
                     QPFAVARAHSGGRNTNFSAIFLDVGGGTTDVAIVKNGNVADTQMFAFGGRVFTKELAR
                     LTGSDFRHAELRKIKYSQKELPKEVMRQVQKTMYTTATLWMRTLKAALESCEDTGPLP
                     TQIYLCGGGALLPDIKSALMEFPWKRYLPIAVVPKIEMFTPDLLGSVIDNSGDLQYIY
                     DITPASLAKFLYDMEIDRKTKNINWEV"
BASE COUNT          497 a          343 c          269 g          719 t
ORIGIN      
        1 ctctggacat actacggtaa catcactttt aagtttatac gatagagttt tacctccaat
       61 ttctgttgtt aaagtatgac cagcctcaag tgtaattggc tgtatttctt ctgtacactc
      121 tggtgagaaa tatatcttta tctttccttc tgccttttct cctttgtatg cctttcctgt
      181 tgcagtaatt gtattagaca aactcttact cttctcttct gttttaattg gtattttaga
      241 attttcaaaa tctatctctt ctacattctc atcccctgat agtacagttt caattttaac
      301 aggctttgac tcaacaaata tttttacttt tacaaatgtt gcaaaatgat tgtaaaggat
      361 tccccctatt attaataaag caagtagagg aataataatc tttaagaaag tttttggtat
      421 tttcttttta ccagcagtag gaatattgtt gtttcgatta aaaggaggct taaatttaaa
      481 gaattttggt ttctcctttt taggttcgct atggatattt tcagtaatta tatctttctc
      541 tatcttaggg atatcttgat caatagaaat aaagggggaa gaagttttaa gtcctctcct
      601 atctacatat tgactcatcc cttcttcttt aggagtttca gtttttttaa cttcttcact
      661 tgctttttct atgacacttt ccactttctc ttcaaaagct tcactctcac tttctttaat
      721 agcagaaatg ttttttaaag tcttctttct ctctagctgt tcttttttct tcctttctaa
      781 tatttcagcc gcctcctgcc aatcatctgc tgtaggatta gatggagaat ctattgcctt
      841 aattcctgct aagagggagt ttctaatacc tgttggattt tgaattattt gagttatgag
      901 gagcttccct tctctttctg ctgtctcatt taagactttt agattaatag ggcttattaa
      961 aatatctgaa tgttctgtaa atgtaagaat tattctttct gctttacttt tatgaatagc
     1021 cctaactaaa tctgttagtt catgtgatgg ttctacaact attttttcta caattgtatt
     1081 gtcttccata ttatacttcc caatttatat ttttagtttt tctatctatt tccatatcat
     1141 acaggaattt tgctaatgaa gcaggtgtta tatcatatat atattgtaaa tctccgctat
     1201 tatcaattac agatccaagc aagtcaggtg taaacatttc tatctttggt acaactgcaa
     1261 tagggagata tcttttccaa ggaaactcca ttaatgcact tttaatatca ggaagaagtg
     1321 caccgcctcc acaaagatat atttgtgtag ggagtggtcc tgtatcttca cagctttcaa
     1381 gagcagcttt taatgtcctc atccatagtg ttgctgttgt gtacatggtc ttttgaacct
     1441 gcctcattac ttcttttggt aattcttttt gagagtattt tatctttctt agctctgcat
     1501 gcctaaaatc agaaccagta agtcttgcaa gttcttttgt aaacaccctt cctccaaatg
     1561 caaacatctg agtatctgca acattaccat ttttaacaat tgcaacatca gtagttcctc
     1621 ctcctacatc aagaaatatc gctgagaaat tagtatttct tcctcctgag tgagctctcg
     1681 caactgcaaa tggctgagat acaataccaa ggacttcaaa attaagagaa gaagcaacct
     1741 ttttaagagc ttctgtatat gttcttgggg caaaagatgc ataaaagtta agttttacat
     1801 tccttccttt atatcctaca agagagtt
//
LOCUS       DAOSAB010000124         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_43395_length_1821_cov_3.037373, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000124 DAOSAB010000000
VERSION     DAOSAB010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43395_length_1821_cov_3.037373"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PK432_02510"
     CDS             complement(<1..1520)
                     /locus_tag="PK432_02510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOK59830.1"
                     /translation="MNVTEESKKRSKKKRFPLLLFIIIPFLSFFIYILVNSGLSVKNI
                     PDLFFYTPPKVKYETFKLSFDESIPQAVVNAIKSSVQNLQFNGTPRFEFVENGGYRVI
                     YTDGLDSAMYFKQYAVVGHIYWIKGDITLNEMKKNKKVYIPSDGDKIHRIFLTQLLGD
                     DVQIVSSDDLASDLIKGEEYVGIVPIEDLNKWMKVLQLDNKYCLEDSRGCLYIGYKLE
                     DRKNSDFVGRIIGRNMEIEYGKSMSVNKNGVAKINMSGVVAMSRGLAQKIDSSGDFAY
                     PARKLGNFLADADLTHVSNEVSFVKGCTGYTGMVFCSKPEYIETLKASGVDIVELTGN
                     HNNDYGAKNNKSSIEKYIELGWDYFGGGLNSEDASKVLYKEVKGNLLAFMGYNWYNTL
                     YSTSALAGSSSAGANSYSVEKLTKDIREAKDKGALAIVTFQFQECYSYPPSDVIYPVC
                     YKPLSSPDQKKVFRQAIDLGADIVIGTQAHQPQTYEVYGDGLIFYGLGNLYFDQDE"
     gene            1594..>1821
                     /locus_tag="PK432_02515"
     CDS             1594..>1821
                     /locus_tag="PK432_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59831.1"
                     /translation="MKKNMTYNAKNVFVTISIFLVIGGVLNIMYERPLNIQNSGKNHI
                     QTAEYIQPAYAKEAVSNTEIDIKPESIKDSQR"
BASE COUNT          634 a          371 c          214 g          602 t
ORIGIN      
        1 cattcatctt gatcaaagta taaattccct aaaccataaa agattagacc atcaccatac
       61 acttcataag tttgaggctg atgtgcctgt gtaccaatta caatatctgc acctaaatca
      121 attgcttgtc taaatacttt tttttgatca ggactgctaa gtggcttata acatacagga
      181 tatataacat ccgaaggagg atatgagtag cactcctgaa actgaaatgt aacaatagct
      241 agtgcacctt tatcctttgc ttctctaata tcttttgtaa gcttttctac agagtaagaa
      301 tttgctccag cagaagaact ccctgcaaga gcacttgtag aataaagagt gttgtaccag
      361 ttatacccca taaatgctaa aaggttccct ttaacctctt tataaagaac cttacttgca
      421 tcctcactat ttaatcctcc cccaaagtaa tcccatccaa gctcaatata tttctcaata
      481 ctacttttgt tattctttgc tccataatca ttattatgat tcccagttag ttcaacaata
      541 tcaacaccac ttgccttaag tgtttcaata tattcaggct tagagcaaaa aaccattcct
      601 gtatatccag tacacccttt tacaaaagaa acttcatttg atacatgcgt taaatctgca
      661 tctgctaaaa agttgcctaa ctttcttgca ggatatgcaa aatctccaga gctatcaatc
      721 ttttgtgcta aaccacgaga cattgcaaca acaccactca tattaatctt cgcaacacca
      781 tttttattta cactcatact ttttccatac tctatttcca tatttctacc aataatccta
      841 cctacaaaat cactattctt tctatcttcc aatttatacc ctatatataa acatccacga
      901 ctatcctcta aacaatattt attatccaac tgtaatactt tcatccattt atttaaatcc
      961 tctattggga caataccaac atactcctca ccctttatta aatcagaagc taaatcatca
     1021 gagctaacaa tctgcacatc atctcccaac aactgagtta aaaatattct atgtatttta
     1081 tctccatcag aaggtatata tactttcttg ttttttttca tctcatttag tgtaatatct
     1141 ccctttatcc aatatatatg ccctactact gcatactgtt taaaatacat tgcactatct
     1201 aaaccatctg tatatatcac cctatacccc ccattctcta caaattcaaa tctaggagtt
     1261 ccattaaatt gcaaattctg tacagatgac ttaatagcat ttactacagc ttgcgggata
     1321 ctttcatcaa aagaaagctt aaaagtctca tactttactt taggtggagt gtaaaagaag
     1381 agatctggga tattttttac tgaaagtcca gaattaacaa ggatatagat aaaaaatgat
     1441 aaaaatggta taataataaa gagaagtaaa ggaaatcttt tcttcttact tctcttttta
     1501 ctttcctcag ttacattcat tctcaacatt aatatcaata tatattattc atttaatttt
     1561 tacaaagctg gggagggtag agaaagaata aatatgaaaa agaatatgac atataatgct
     1621 aaaaatgtat tcgttactat atcaatattc cttgttatag gaggagtact aaatataatg
     1681 tatgaaagac cacttaatat ccaaaacagt ggtaagaatc atatacagac agctgaatat
     1741 atacagcctg cttatgcaaa agaagcagtt tcaaatacag agatagatat taaacctgaa
     1801 agtattaagg attcacaaag a
//
LOCUS       DAOSAB010000125         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_44504_length_1790_cov_1.931412, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000125 DAOSAB010000000
VERSION     DAOSAB010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44504_length_1790_cov_1.931412"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PK432_02520"
     CDS             complement(<1..660)
                     /locus_tag="PK432_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HOK59832.1"
                     /translation="MERVPPQNIDAEKSVLGSILLDRDALIEVSGWLSPAHFYDERHI
                     AIYETILELFESGLPIDLVTVSDRLKKKKMLSKIGGRAYLTELASFVPTAAHAVEYGN
                     IVKETATRRGLISSSSEITKLAFDESLALPEVVDRSETLLFNIAQEGIQSNFVHIKDL
                     LKDAYERASHAKESDDYSGISTGFKELDVLLGGFQKSDLIVLAARPSVGKTALALDMM
                     RL"
     gene            complement(666..1313)
                     /locus_tag="PK432_02525"
     CDS             complement(666..1313)
                     /locus_tag="PK432_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK59833.1"
                     /translation="MKIKSLGWSSFSLSNDNISILTDPVALEESGIGLPKCKADVLLF
                     SQKDLYGKEDILKEYELEKKIEPDHRENIFEISSPVEYEIGGMMIRRAINLPFYTIDE
                     GNIRVVYMGIIDNSLDVSKTKDLGDVDVLILPIGNGDLFIDYEKIEKILSYIDPTILL
                     PCAYKKDGLKVGKNIKSRDDFIKYFGFTNVKDQSSLNVTPTVEQESKIMEVIFLN"
     gene            complement(1374..1586)
                     /locus_tag="PK432_02530"
     CDS             complement(1374..1586)
                     /locus_tag="PK432_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOK59834.1"
                     /translation="MKYPKRIQNRVNRIEGRIKRMMMSDTDEDKVMIQLQAVISSLES
                     LKLELVQKQMKESLMEDIKKSLGLSD"
BASE COUNT          594 a          363 c          216 g          617 t
ORIGIN      
        1 aagcctcatc atatcaagtg ctaatgctgt cttaccaact gaaggtcttg cagcaagaac
       61 aattaaatca gacttctgga acccgccaag taagacatct aactctttaa aacctgttga
      121 tatacctgaa taatcatcac tctctttagc atgagatgct ctttcatatg catctttaag
      181 caaatcctta atatgtacaa aattactctg aatcccttcc tgagcaatat taaaaaggag
      241 agtttctgac ctatcaacaa cttcaggtag agcaagactc tcatcaaatg caagttttgt
      301 tatctctgaa gatgaagaaa tcaagcctct tctggtagct gtttctttaa caatattccc
      361 atattcaacg gcatgagcag ctgtaggaac aaaagatgct aactctgtaa gatatgctct
      421 tccacctatc ttacttaaca tctttttctt ttttaatcta tcagaaacag taacaagatc
      481 tatagggaga ccactttcaa aaagctcaag aatagtctca tatatcgcaa tatgtctttc
      541 atcataaaag tgtgcagggg aaagccagcc agaaacttca ataagggcat ctctgtctaa
      601 caaaattgac cctagtactg acttttctgc atctatattt tgtggtggta ctctttccat
      661 attttttaat taagaaatat aacctccatt attttactct cctgctctac tgtgggggta
      721 acatttaaag atgactgatc tttaacattt gtaaatccaa aatatttaat aaaatcatct
      781 ctacttttaa tatttttccc cacttttaac ccatcttttt tatatgcaca gggcagcaga
      841 atagtaggat caatatagct aagaatcttt tcaattttct cataatctat gaacaaatct
      901 ccattcccta ttggaagtat tagcacatct acatcaccta aatcttttgt ctttgaaaca
      961 tctaatgaat tatcaataat tcccatatat acaactctta tatttccttc atctattgtg
     1021 taaaaaggaa gattaatagc tcttctaatc atcatacccc ctatttcata ctccactggg
     1081 gatgatattt caaagatatt ttctctgtga tcaggctcaa ttttcttctc taactcatac
     1141 tctttcagta tatcttcctt accatacaaa tctttttgag aaaaaagaag aacatctgct
     1201 ttacacttag gtaaaccaat accactttct tcaagtgcca cagggtctgt taatattgat
     1261 atattatcat tacttaaaga aaaagatgac caaccaagac ttttaatctt catatttgca
     1321 catactaatc ttaacaaggg cagtacatat actctgtaaa agtatatcaa tttttaatca
     1381 gaaagtccta aagatttttt aatatcctcc attaaagact ctttcatctg tttttgaacc
     1441 agctctaact ttaaactctc tagagaagaa atcacagcct gaagctgaat cataactttg
     1501 tcttcatctg tatcactcat catcattctt tttatccgtc cctctattct atttaccctg
     1561 ttttgaattc tttttggata tttcatatta gattagtata ttgagaaatt atacaaaaag
     1621 caacctttaa ccccttgaca caattagcac tcgatgctat tctttgctaa tggtaaaacc
     1681 atatttaaat tttaatatta aaacatatga aaatagtaaa agttaatcaa aaaccacagg
     1741 atacactata cactcctgta agatcattca tggatgaact ctttaattat
//
LOCUS       DAOSAB010000126         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_45136_length_1773_cov_2.030850, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000126 DAOSAB010000000
VERSION     DAOSAB010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45136_length_1773_cov_2.030850"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1773
                     /locus_tag="PK432_02535"
     CDS             <1..>1773
                     /locus_tag="PK432_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59835.1"
                     /translation="FNKHNIYIAHITAELKEKLATRGGRMGHHHDQRFNISGYKGSEL
                     GLTPEELGHSFKQGIQDYVQEFQSQYKSMIIRHKIDPILEAQGFRDSHPQTHAAIQQI
                     KSSAINIVSNKVEAFDRSIYESTDRVARVIYESLYPNKLFNPKDPVYKTAQSAAISTF
                     YLFKVLPSLAMSVIQLFNPVIAMRHGAIDNGFLTLGSFGKGMFKLFTGDSELMQSLHQ
                     VTQTTDVIEPQFLNTLHMKGENKLLEGLKDWVAMRKPQEACDIISRTMGYAYFYTHYK
                     DIGHTPLEAKTLALEAIDANFGAYTRGETAPIFQHLGGIIGEGMRPLQTFGQMTVGNL
                     VADVKRMFDNPKKLKSYAPFIMYGLTATLLGGAVTGPIMNQYETMRQFFMKMKPEWDI
                     PSALDLTLKGVISLDNVVEDKEALTKLIAYGVLSQQTGIDIGASARTTETLPGTMLSV
                     LLSMVEGDKAATEIAKEAGRLMPVHSNIIHMASGAETLAKSLVTDVPKNELKQGITDV
                     AMRGPMKNALMETTGANKTAVMGKDTNMIASGSDSSALMEEGPKEKVAHWMGNMSTKE
                     RFRTDQKIEDQFRTKAFNDKIKRM"
BASE COUNT          595 a          259 c          341 g          578 t
ORIGIN      
        1 atttaataaa cataatatat atatagccca tattacagca gaattaaaag aaaaacttgc
       61 aactcgtggt ggtaggatgg gacaccatca tgatcaaagg tttaatattt ctggttacaa
      121 aggatctgaa ttaggactta ctcctgaaga attaggacat agttttaaac aaggtataca
      181 agattatgtg caagagtttc aatctcagta taaatctatg ataattagac acaagattga
      241 tcctatatta gaagctcagg gatttagaga tagtcatcca caaacacatg ctgctataca
      301 acagataaaa tcctctgcta ttaatattgt atctaataaa gtagaagctt ttgatagatc
      361 tatttatgaa tctacagata gagtagctcg tgtaatttat gaaagtttgt atcctaataa
      421 actctttaat cctaaagatc ctgtatataa aactgcacaa agtgcagcaa taagtacttt
      481 ctatttattt aaagttttac cctctttagc tatgagtgta atacagttat ttaatcctgt
      541 tattgctatg cgacatggag ctatagataa tggttttctt actctaggtt cttttggtaa
      601 aggtatgttt aaattattta ctggtgattc agagttaatg caatctttac accaagtaac
      661 tcaaactaca gatgttatag aaccacagtt ccttaatact cttcacatga agggggagaa
      721 taaacttctt gaaggtttaa aggattgggt agcaatgagg aaaccacaag aagcttgtga
      781 tattatttct agaactatgg gatatgctta tttttatact cattataaag atataggtca
      841 tacaccatta gaagctaaaa cactagctct tgaagctatt gatgcaaact ttggtgcata
      901 tactagagga gaaactgctc ctatatttca acatcttggt ggtattatag gtgaaggtat
      961 gcgtcctctt caaacatttg gacagatgac tgttggtaat cttgttgctg atgttaaaag
     1021 aatgtttgat aatcctaaga agttaaagtc ttatgcacct tttattatgt acggacttac
     1081 tgcaacactc ttggggggag cagttacagg tccaataatg aatcaatatg aaacaatgcg
     1141 tcagttcttt atgaagatga aacctgagtg ggatatacca tctgctcttg atcttacttt
     1201 aaagggtgta attagtcttg ataatgttgt agaagataag gaagctttaa ctaaacttat
     1261 tgcttatggt gttctttcac aacaaactgg tattgatatt ggtgcttctg ctagaactac
     1321 tgagacttta cctggtacta tgttatcagt tcttctttct atggttgaag gtgataaagc
     1381 tgctactgaa atagcaaaag aagcaggaag gcttatgcct gtacattcta atattattca
     1441 catggcttct ggtgctgaga ctttagcaaa aagtcttgtt acagatgttc ctaagaatga
     1501 gttaaaacaa ggtattactg atgtagctat gcgtggtcct atgaaaaatg ctcttatgga
     1561 aactacaggg gctaataaga ctgctgtaat gggtaaagat actaatatga ttgcttctgg
     1621 ttctgatagt tctgctctta tggaagaagg accaaaagag aaggtagcac attggatggg
     1681 taatatgtct actaaagaga ggtttagaac tgatcaaaaa attgaagatc agtttagaac
     1741 taaagccttt aatgataaaa tcaagagaat gtt
//
LOCUS       DAOSAB010000127         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_45350_length_1767_cov_2.453271, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000127 DAOSAB010000000
VERSION     DAOSAB010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45350_length_1767_cov_2.453271"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..868)
                     /locus_tag="PK432_02540"
     CDS             complement(110..868)
                     /locus_tag="PK432_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59836.1"
                     /translation="MHESILAQQRGIVLSVLKCDKISAAMKNGRLYINWLVVITLFLF
                     ITSLGVFLMYIQKNLVSDQCAYGQDMGENCVCSSDGVKVCEGELPVKDRSVSEFTSNG
                     LTYSVNFLNLVSLNNPPKHSVEFTDILQSGNMLRVVVDLNSMCNSNNKVAPQIGFYKL
                     DNERLILTVISNLTDSSFNLQCRSENIFLIENFDLNSKETFKLQFQDELGNIYQASTC
                     IYQGYIRNDGDVYVSKDSQMLCRCNNGVNSCEKR"
     gene            863..1765
                     /locus_tag="PK432_02545"
     CDS             863..1765
                     /locus_tag="PK432_02545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOK59837.1"
                     /translation="MHDSHIHISMSPLKENIHEDIQEFIELGGKKILAQTTDMTNYQD
                     TIDMYKHLNSKFPDTIDLALGIHPTRIEEGISKNELDSVDIFQYMKKRYEIFEEVLNK
                     NIKDVTAIGECGLDYFSMNQYFNFTQEKKKQIKEAQRLLFSKLCKLAVKYNLPMSIHA
                     RDIDGSSNCVKDVLAIIAKEGKGTIKGSFHSYTGDIKMLDKILSLGMYVGFNAIITYP
                     SGENVREILKEAPVERVLFETDGPFLPTQSVRKRKNSIKKYGRPALIKEIIEKACEVK
                     NIEYKRMEEITDNNYFTLFSKQDK"
BASE COUNT          677 a          277 c          263 g          550 t
ORIGIN      
        1 tcatcatttt caagcaatgg gatggccaca gtataaaata acaatgaggt tttggctgtt
       61 tacattattt ggctgtatat tagaattata tataggagtg ctttaatagt tatctctttt
      121 cacaactatt tactccatta ttacaacggc acagcatttg actatctttt gaaacatata
      181 catcaccatc attccttata tacccctgat atatacaggt tgatgcttga taaatattcc
      241 ctaattcatc ttgaaactgt aatttaaaag tttccttaga atttaaatca aagttctcaa
      301 ttaaaaagat attctctgat ctacattgta gattaaatga agagtctgtt aaattactaa
      361 ttactgtaag aattagtctt tcattatcca atttgtaaaa tccaatctga ggagctacct
      421 tattattaga attacacata ctatttaggt cgacaacaac cctaagcata tttccacttt
      481 gcaatatgtc tgtaaactca acactgtgtt taggaggatt atttaaacta actagattta
      541 aaaaattaac agaatatgtt aaaccatttg aagtaaactc actaacactc ctatctttca
      601 ctggcaactc tccctcgcac acttttacac catcactact acatacacag ttctccccca
      661 tgtcctgtcc atatgcacat tgatcagaaa caagattttt ctgtatatac attaagaaaa
      721 cccctaaaga ggtaataaag agaaacaaag taataaccac caaccagtta atatacaacc
      781 gaccattttt cattgcagcc gatattttat cacatttcaa aactgaaagc acaattcctc
      841 tctgttgtgc taaaatactc tcatgcatga ttcacatatt catatatcaa tgagtccatt
      901 aaaagagaat atacatgagg acatacaaga gtttattgaa cttggtggaa agaagatact
      961 tgcacaaaca acagatatga caaattatca agacaccatt gatatgtata aacacctaaa
     1021 cagtaaattc ccagacacca ttgatttagc cttagggata catcctacaa ggatagaaga
     1081 aggaatatca aaaaatgagt tagacagtgt agatatcttt cagtatatga aaaaacggta
     1141 tgagatattt gaggaagtat taaacaaaaa tattaaagat gtaactgcaa taggggagtg
     1201 tggattggat tactttagta tgaatcaata ttttaatttt acccaagaga agaaaaaaca
     1261 gataaaagaa gcacaaaggt tattattttc taaattatgt aaattagctg ttaaatataa
     1321 tctcccaatg agtattcatg caagagatat agatggaagc agtaattgtg ttaaagatgt
     1381 acttgcaata attgcaaaag aaggaaaggg aacaattaaa ggttcttttc atagctatac
     1441 aggtgatatt aaaatgcttg ataaaattct tagcttaggc atgtacgttg gttttaatgc
     1501 aataattaca tatccaagtg gtgagaatgt aagagaaata cttaaagaag caccagttga
     1561 aagagtactt tttgaaactg atggtccttt tctccctact caaagtgtaa gaaaaaggaa
     1621 aaactctatt aaaaaatatg ggagacctgc tttaattaaa gaaattatag agaaagcatg
     1681 tgaagttaaa aatattgaat acaaaagaat ggaagagata acagataata attattttac
     1741 tcttttttca aaacaggata agtaatt
//
LOCUS       DAOSAB010000128         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_45629_length_1759_cov_2.323944, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000128 DAOSAB010000000
VERSION     DAOSAB010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45629_length_1759_cov_2.323944"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1759
                     /locus_tag="PK432_02550"
     CDS             <1..>1759
                     /locus_tag="PK432_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59838.1"
                     /translation="KTGREFGSDDDISKDTVISKNIYDGVDIQYQIIKGKGLKEEIVL
                     NEIPQYTTECSKEECVLPVNRFVFEIKLDEGLDIKKSISATDKYPNGVVYIVDEKGNY
                     YAHFLPEYAADAVGVKTSSVKSSIVKSGDKYIYEIVLDSKWLLDKSRVFPIKIDPSIV
                     HDTQFVFEQGQFDRVKLNDVMNIELDNALSGQYTSPSVTVGNNAVVKGIEWQSFGQAT
                     EDEQTPFSRLDLVAEYNFDDEQSFKKKYGEGSLQLEQGESRQFTVNSNSSGYITVQFW
                     SYKRRMSANSELFSSNLGKLVIENDKYVFKDNDNISHNTEIEVEYNRWQYITIVFGIT
                     NSNVTVYVDNKEFSADISYSSQNNLGNIQLFGSGYFDVLRVYERMLASNEILSDSGYS
                     DIYLEYMTSDDNLSWSNWISKSQHPIPLAFENENMDIVLDGVDISKYNLLSLQFLAKE
                     EGYIVVGGSKFSNGATNQDIHSLVDAEGTLDNAEQIKYIDLVFKPSSLQDSCLLSVGD
                     VNIKVLQTGKVVVLYDEESNELVDLYIPEKNNYLSISFLDDKTKIYLNGWLSFSGEPY
                     VLNANEYKIGLGCSDVQNA"
BASE COUNT          602 a          191 c          391 g          575 t
ORIGIN      
        1 caaagacagg cagagagttt ggaagtgatg atgatatatc aaaagatact gtaatatcta
       61 aaaatatata tgatggagtt gatattcagt atcaaataat aaaaggaaaa ggtcttaaag
      121 aagagattgt tttaaatgag ataccccagt atacaactga atgcagcaag gaagagtgtg
      181 tactgcctgt taataggttt gtttttgaga ttaagcttga tgaaggtttg gatattaaaa
      241 agagtattag tgcaactgat aaatatccca atggagttgt atatattgtt gatgaaaagg
      301 gaaattacta tgctcatttt cttcctgaat atgcagctga tgctgtcggt gttaaaacaa
      361 gcagtgttaa atctagtatt gtaaaaagcg gagataaata tatatatgag atagttttag
      421 attctaagtg gttgcttgat aaaagtagag tgtttccgat aaagatagat ccaagtattg
      481 ttcatgatac acagtttgtg tttgagcagg ggcagtttga tagggttaaa cttaatgatg
      541 ttatgaatat tgagcttgat aatgctttgt cagggcagta cacatcgcct tctgttactg
      601 ttggaaataa tgcagtagtc aagggtattg agtggcaaag ttttggtcag gcaactgagg
      661 atgagcaaac tcctttttca agattagatc ttgtagcaga gtacaacttt gatgatgaac
      721 agagttttaa aaagaagtat ggtgagggct ctttacagct tgagcaagga gagagtaggc
      781 agtttactgt aaacagtaat tcatctgggt atataactgt acagttttgg tcttacaaaa
      841 ggagaatgtc tgcgaatagt gaattatttt catctaattt aggaaaactt gtaattgaaa
      901 atgataagta tgtatttaag gataatgata atatatccca taatactgag atagaggtag
      961 agtacaatag gtggcagtat attactattg tttttggtat aacaaactct aatgtaacag
     1021 tatatgttga taataaggag ttttctgctg acatatcata cagttctcag aataatttag
     1081 ggaatataca actcttcggg agcgggtatt ttgacgtttt aagggtgtac gagagaatgc
     1141 ttgcaagtaa tgagatttta agtgattctg ggtattctga tatatatctt gaatatatga
     1201 caagtgatga taatttatcc tggagtaatt ggatatcaaa gtcacagcat cctataccct
     1261 tagcttttga gaatgagaat atggatatag tgctagatgg tgttgatatt tcaaagtaca
     1321 atttactttc acttcaattt ttagcaaaag aggaaggata tattgttgta ggaggaagca
     1381 aatttagtaa tggggcaacc aatcaagata tacacagcct agtagatgca gaagggacat
     1441 tagataatgc agagcagata aagtatattg atctcgtatt taaaccctct agtttacaag
     1501 attcatgtct acttagtgta ggagatgtga atattaaagt tttacaaaca ggaaaagttg
     1561 tagttttgta tgatgaagaa agcaatgagt tggtagattt atacatacct gagaagaata
     1621 attatctttc tatttctttt ttagatgata aaacaaagat atatttaaat ggttggcttt
     1681 catttagcgg tgagccatat gtgttaaatg ctaatgagta caaaattggt ttagggtgta
     1741 gtgatgtgca gaatgcatt
//
LOCUS       DAOSAB010000129         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_45690_length_1757_cov_3.397767, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000129 DAOSAB010000000
VERSION     DAOSAB010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45690_length_1757_cov_3.397767"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK432_02555"
     CDS             <1..181
                     /locus_tag="PK432_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOK59839.1"
                     /translation="TLAVRWIVEVSRSKSGKSFDKILEEEMINAFKGEGDAVKKKNDV
                     ERMAEANKAFAHFRW"
     gene            complement(182..1120)
                     /locus_tag="PK432_02560"
     CDS             complement(182..1120)
                     /locus_tag="PK432_02560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK59840.1"
                     /translation="MISIIITSWKEPKTIGKCIECIVDRKYSGLNENFEVIQVSPDKE
                     TLEAGLYTAKKLHLNKKEFIQIQDPRKGKPSALKLAFKKAKGDIWILTDGDTYFEKNA
                     VKYLLEPFQNNKVGGVSGRPVAMNKKDSMFGYWGHLLSDAAHHRRVITMNKKEGNYYL
                     SDKEFFPMSGYIMAVRNFNFDIPPNVLSDDAYLSYSIRNIGKEIAYAPLAKCYVKYPT
                     NLKDYYKQKVRSLGGFIQLKEFGVFQKDKQSRSFLIELKYASFVLKYAANLKEFIWSL
                     TLFPVRLITWIKIFWERVVLKKDMPKTGWERIQSTK"
     gene            complement(1160..>1757)
                     /locus_tag="PK432_02565"
     CDS             complement(1160..>1757)
                     /locus_tag="PK432_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOK59841.1"
                     /translation="TPAVVYDSHGQSINVTMSSSDAQWLYKNTTSTTILDTQLEKESF
                     KALVKEFSINPVTDEINHVSLFRIDENVPMVFTIPFKIVGVSPAVKNNIGVLVNVLDS
                     IDVKCKMADLVPYIEIDISNLENVGQTIAVDDIQLPKGISLINDEQAKAAIVTITEPQ
                     KVEEIQPTATEGSVEEEVTEGEEGAETAQEEQTTSEDK"
BASE COUNT          526 a          310 c          257 g          664 t
ORIGIN      
        1 aacattagca gtaagatgga tagttgaagt atcaagatca aaatcaggaa aatcctttga
       61 taaaattctt gaagaagaga tgataaatgc atttaaagga gagggagatg cagttaagaa
      121 gaaaaatgat gttgagagaa tggctgaagc taacaaggca tttgctcatt ttagatggta
      181 attattttgt actttgaatt ctttcccagc ctgttttagg catatctttt ttaagtacga
      241 ctctttccca aaatatcttt atccatgtta ttagtcttac aggaaacaat gttaatgacc
      301 atataaattc tttaaggttt gctgcatatt ttaagacaaa agaagcatat tttaattcaa
      361 taagaaaact tctactctgt ttatcttttt gaaaaactcc aaactccttt aactgtataa
      421 atcctcctaa ggatctaacc ttttgcttgt agtaatcttt taaatttgtt ggatacttta
      481 cataacattt tgctaaagga gcgtatgcta tctcctttcc aatattcctt attgaatatg
      541 ataaatatgc atcatcagat agcacattag gagggatatc gaaattaaaa tttcttacag
      601 ccataatata tccactcata ggaaagaatt ctttatcact gagatagtaa ttaccttctt
      661 tcttgttcat agttattact cttctatgat gtgctgcatc agaaagaaga tgtccccagt
      721 atccgaacat actatctttt ttattcattg ctactggtct tccacttact cctccgactt
      781 tattattttg aaatggttca agtaaatatt taactgcatt cttttcaaaa tatgtatctc
      841 catctgtaag aatccagata tctccttttg cttttttaaa tgcaagtttt aatgcagatg
      901 gcttgccctt tctagggtct tgaatctgta taaactcttt tttgtttaag tgaagtttct
      961 ttgctgtata tagacctgct tctaatgttt ctttgtctgg agatacctgt atgacttcaa
     1021 aattctcatt tagtccagag tattttctat ctactataca ctctatacat ttaccaatag
     1081 tcttaggttc tttccatgat gtaataataa tagaaatcat ggttgattat acaggaaaat
     1141 ggtggaattt acataccttt tacttatctt cactagttgt ttgctcttct tgtgcagttt
     1201 ctgcaccctc ttcaccctct gttacttcct cttcaactga tccttctgtt gctgtaggtt
     1261 gtatctcttc aaccttttga ggctcagtta ttgtaacaat agcagctttt gcttgttcat
     1321 catttataag ggatattccc tttggaagtt gaatatcatc aaccgctata gtttgtccta
     1381 cattttctaa atttgatata tctatttcaa tgtaaggtac taaatctgcc attttacact
     1441 ttacatcaat actgtctaaa acatttacca ataccccaat attattttta actgcaggtg
     1501 atactccaac aatcttaaat ggtattgtaa ataccattgg tacattctca tctattctaa
     1561 atagcgatac atgatttatc tcatcagtta ctggattaat actaaactct ttaacaagtg
     1621 ccttaaaact ctctttctca agctgtgtgt caagtatagt agtggatgta gtgtttttgt
     1681 agagccactg tgcatctgaa ctactcatag taacatttat agattgtcca tgagaatcat
     1741 atacaacagc aggggtt
//
LOCUS       DAOSAB010000130         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_45738_length_1756_cov_2.239271, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000130 DAOSAB010000000
VERSION     DAOSAB010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45738_length_1756_cov_2.239271"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PK432_02570"
     CDS             <1..798
                     /locus_tag="PK432_02570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK59842.1"
                     /translation="SVVDTDYKDFDIVVVDNNSHDGSVQYLKKNFPSVKVVESDENLG
                     FGRGNNLGVSMYPDYDAYLFLNNDVSVPKEWLKELVKVLESDDSIGAVGPKILYSKKR
                     NGKYIVNSAGIEVDKHFMAYDRYDKQEDNPKYDVIQEVDALMGSAFLVRREVFEKAGG
                     FNPKMFLYYEDVDLSLRVRDLGYKLVYCGKSKVFHDHMASSKKLTMRKRNIMNMKNRY
                     ISIKSRLGVWVAIKETIWYLFNWLIWKMIYSKRMSLKQFFKKNNETE"
     gene            785..1558
                     /locus_tag="PK432_02575"
     CDS             785..1558
                     /locus_tag="PK432_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK59843.1"
                     /translation="MKQNDIDLYKQLVKTIFKMRYKGSILGFLWVLLKPFFMFLILYV
                     IFSATAGAAGGLTSRQYAVYLLSGLIIYTFFNEGFTWGMNSIMDRADLILKINFKRSI
                     VVISALTMALINFVINFFIIATIGVVLDINYSLNGLLYVFLICIVMFLGMYGISFFTS
                     IWRVYVRDLTHISELVLQLLFYASAVFFPIEMVPENYRFIISYNPLAVFIQAVRNALI
                     YGEITHLNFVLASLVVSIVLVFVGRAYFEKHILKVAEHF"
     gene            1560..>1756
                     /locus_tag="PK432_02580"
     CDS             1560..>1756
                     /locus_tag="PK432_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK59844.1"
                     /translation="MEDIAISVKNVSKKFRIPINGKKETLQERFVNPFHKTEYVEFYT
                     LNNLSFDVKKNEFFSVIGPNGA"
BASE COUNT          569 a          194 c          350 g          643 t
ORIGIN      
        1 tctgttgtag atactgatta caaagatttt gatattgttg tggttgataa taattcacat
       61 gatgggagtg tacagtatct taaaaagaat tttcctagtg taaaagtagt tgaaagtgat
      121 gagaacttag gctttggaag aggtaataat ttaggagttt ctatgtatcc tgattatgat
      181 gcatatcttt tcttaaataa tgatgtttca gtacctaaag agtggttaaa agagcttgtt
      241 aaagttttag aatctgatga cagcataggg gcagttggac caaagatttt gtactcaaag
      301 aagagaaatg gcaagtatat tgttaactct gctggcatag aggttgataa acattttatg
      361 gcatatgaca ggtatgataa gcaggaagat aatcctaaat atgatgttat acaggaggtt
      421 gatgctttaa tgggtagtgc atttttagtt agaagagagg tttttgagaa agcaggtggt
      481 tttaatccga agatgtttct ttattatgaa gatgtagatt tatccttaag agttagagat
      541 ttaggataca agcttgtata ttgtggtaaa tcaaaagtat tccatgatca tatggcttct
      601 tctaagaagc tgactatgag aaaaagaaat attatgaata tgaaaaacag atacattagt
      661 attaaatcaa gattaggagt atgggtagca attaaagaaa caatatggta tctttttaac
      721 tggttaatat ggaaaatgat atactcaaaa agaatgagct taaaacagtt ttttaaaaag
      781 aataatgaaa cagaatgata tagacctata caaacagttg gttaaaacga tatttaagat
      841 gagatataaa gggtccatct taggctttct gtgggttttg cttaaacctt tttttatgtt
      901 tctaatcctt tatgtaatct tctcggctac tgcaggtgca gcaggaggtc ttacatcaag
      961 gcagtatgca gtatatcttt tatcaggtct tatcatctat acattcttta atgagggttt
     1021 tacatggggt atgaactcca taatggatag agcagattta attttaaaga ttaactttaa
     1081 aagaagtatt gtggttataa gtgcactgac aatggcttta atcaactttg taatcaactt
     1141 ttttattatc gctacaattg gtgttgtttt agatattaat tactctctta atggtttgtt
     1201 gtatgtattc ttaatatgta ttgtaatgtt tcttggtatg tatggtatat ccttttttac
     1261 aagtatatgg agggtatatg taagggacct tactcatatt tctgagcttg tgctacagct
     1321 tttgttctat gcttctgctg tatttttccc aatagagatg gttcctgaga attatcgttt
     1381 tatcatttct tacaatcctt tagctgtatt tatacaagct gtgagaaatg cattaatata
     1441 tggtgagatt actcatttga actttgtatt agcttctctt gttgtttcca ttgttcttgt
     1501 ctttgtaggc agagcatatt ttgagaagca tattttaaaa gtagcagaac atttttaata
     1561 tggaagatat agctataagt gtaaaaaatg ttagtaagaa atttagaatc cctattaatg
     1621 ggaagaaaga aactttgcag gagaggtttg ttaatccttt ccataaaaca gagtatgtag
     1681 aattctatac tttaaataat ctatcttttg atgttaagaa gaatgagttt tttagtgtaa
     1741 tagggccaaa tggtgc
//
LOCUS       DAOSAB010000131         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_46011_length_1748_cov_2.604843, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000131 DAOSAB010000000
VERSION     DAOSAB010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46011_length_1748_cov_2.604843"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PK432_02585"
     CDS             complement(<1..420)
                     /locus_tag="PK432_02585"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOK59845.1"
                     /translation="MDLNILKQKYFFMPDATRGAVRYLTVDQLKQTGTQAIVTNTLHL
                     LISPGPDVIQKLGGIKGFMNWDGIVFTDSGGFQLFSLLHSHKWEGKVHENGAVFKSPR
                     EGNTYELTPETSIDIQIKIGSDVLVCLDDCRDTNLTRY"
     gene            complement(425..1435)
                     /gene="queA"
                     /locus_tag="PK432_02590"
     CDS             complement(425..1435)
                     /gene="queA"
                     /locus_tag="PK432_02590"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOK59846.1"
                     /translation="MKMSEFKYNLPKELIAQHPPQKRGTSNLLVLDRKSGEISHKKYF
                     NIPDFVQKGDVIVLNETKVLNCRTFFLTPRNKKVEVLFLEERDNNIWFCLVGRAKDIH
                     ISDVLINNEIRIEVLKREDNGFLVKFISKDAYTLFEKYGHVPLPAYMKREDTEDDKER
                     YNTVFSDIPGAVAAPTASLNLTDEILFNIKKKGAEIAKIELKVGWGTFSPVREENVED
                     HKIHREYINVSKQSADIVNECKGRVWAFGTTVVRALESAYSKEEKKVVPFSGYTDLYI
                     YPGFKWRVVDVLVTNFHMPDSSLILLVTAFAGKENIKRAYEEAIKRKYKFLSYGDSML
                     IK"
     gene            1488..>1748
                     /locus_tag="PK432_02595"
     CDS             1488..>1748
                     /locus_tag="PK432_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59847.1"
                     /translation="MRNLKEEPIQTLFLGTDWESTQILNALYNDKRFKITGVITNEDK
                     LIGRKQILTPSKVKEFALKHNIEVLHTNSDTEKYKEALERFNP"
BASE COUNT          591 a          329 c          232 g          596 t
ORIGIN      
        1 atatcttgta agattagtat ccctacaatc atcaagacac actaaaacat cagaccctat
       61 ctttatttga atatctattg atgtctcagg agtaagctcg tatgtatttc cttctcttgg
      121 agatttaaaa acagctccat tctcatgaac ctttccttcc catttatgag aatgaagaag
      181 agaaaaaagc tgaaacccgc ctgaatctgt aaacacaatc ccatcccaat tcataaaccc
      241 ctttataccc cctaattttt gtattacatc aggaccagga gagattaaaa gatgaagagt
      301 atttgtaaca atagcctgtg tacctgtctg cttaagctga tcaacggtta aataccttac
      361 agcacccctt gttgcatcag gcataaagaa atatttttgc tttaatatat ttaaatccat
      421 atctttactt aattaacata ctatcaccat agcttaagaa tttgtatttt cttttaattg
      481 cttcttcata tgctctttta atattttcct tacctgcaaa agcagttaca agcaaaatta
      541 aagaagaatc aggcatatga aaattagtta ctagtacatc aacgactctc catttaaacc
      601 caggatatat gtataaatcg gtatatccag agaaaggtac aacctttttc tcttcttttg
      661 aatatgcgct ttcaagagct ctaactactg ttgtaccaaa tgcccagact cttcctttgc
      721 actcgttaac aatatctgca ctctgtttac ttacattaat atattcacga tgtattttat
      781 gatcctcaac attctcctct ctaacaggag agaaggttcc ccatccgact tttaattcaa
      841 ttttagcaat ttcagctcct ttctttttaa tattgaaaag tatctcatct gttaaattta
      901 aacttgcagt aggagcagca acagcaccag gaatatcaga gaatacagta ttgtatcttt
      961 ctttatcatc ctctgtatct tctctcttca tatatgcagg aaggggaaca tgtccgtatt
     1021 tctcaaacaa agtgtatgca tctttactga taaactttac aagaaaacca ttatcttctc
     1081 ttttcaagac ttcaattcta atttcattat taatcaatac atcagatata tgtatatctt
     1141 ttgctcttcc tacaagacaa aaccatatgt tattatctct ttcttcaaga aagagaactt
     1201 caaccttttt attccttgga gttaaaaaga atgttctaca atttaatacc tttgtctcat
     1261 taagaactat cacatctcct ttttgtacaa agtcaggaat attgaaatat tttttatgag
     1321 atatctctcc actctttcta tctaagacaa gcagattgct tgtgcctctt ttttggggag
     1381 gatgctgagc aattaactct ttaggtaaat tatatttaaa ttcagacatt ttcataacta
     1441 tggattatat agcaatttgg gctgtattac agtaaaatca tctatatatg agaaatttaa
     1501 aagaagaacc aatacaaaca ttgtttttag gaacagactg ggaaagtaca cagattctta
     1561 atgcacttta caatgataaa agatttaaaa ttacaggggt tattacaaat gaagataagc
     1621 ttataggaag aaaacaaatt cttactcctt caaaggtaaa agagtttgca cttaagcata
     1681 atattgaagt gctccataca aacagtgata cagaaaagta taaagaagca ttagaaagat
     1741 ttaatcct
//
LOCUS       DAOSAB010000132         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_49511_length_1661_cov_2.926526, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000132 DAOSAB010000000
VERSION     DAOSAB010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49511_length_1661_cov_2.926526"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="PK432_02600"
     CDS             complement(<1..1560)
                     /locus_tag="PK432_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59848.1"
                     /translation="MNAECRSDCYQHKYCGCVGDGGCVYRGTCNQYEASLNCGEAGTM
                     CCDRGYCESCTPVCDANYQSSDNGCSAASFSCTKYDCIGSCGTASITCYRKKYSVSIT
                     GNGGTCSPLSPSEVCGDSPSPAPAAGSCTRTGYTVSGYSIYSGSCGGTFNSSTGVCSK
                     VTGPLVIQPNWTINYYTISALGNGGTCSPSTQSISHGSASGVVTCTRSGYNFTGFTIS
                     PSGNCGGTFNSSTGVCSSVVGAFSITAQWVIANQAPTAPTTPYCMGTTNPTNVTTQTP
                     YFSAIFNDPDTGNTGNYYEIEVNTASGFNGTVMWDSGSQSMTATAIGSRSPNIYYDGS
                     SLGWSGTTYYWRIRFWDNGGLVSPWSAVQNFKMHSNSAPTAPTNLQTESATNPVQVTD
                     TTPEFRAIFNDPNTGDTGIQYEIEVNTASDFSGTVMWDSGLQSMTSTPIGSYSPEISY
                     SGTPLTLNGATYYWRIRFVDNYGYVGAWSATANFQMNNIPTATSPKTNGQTNPPKVPM
                     TPYFTAVFNDAD"
BASE COUNT          491 a          339 c          289 g          542 t
ORIGIN      
        1 atctgcgtca ttaaatactg ccgtaaaata cggagtcatt ggtacttttg gcgggtttgt
       61 ttgaccattt gttttgggac ttgttgcagt tggtatgtta ttcatttgaa agttagcagt
      121 tgcagaccaa gctccaacat atccatagtt atctacaaat ctaattctcc aatagtaggt
      181 agcaccattt aaagtaagtg gtgtcccact atatgatatc tctgggctgt aacttcctat
      241 tggtgttgat gtcatacttt gtaaaccact atcccacata actgttccac taaaatcaga
      301 ggcagtattt acttctattt catactgtat accagtgtct ccagtatttg gatcattaaa
      361 tattgctcta aactctgggg tggtatcagt aacttgtaca gggtttgtag cagattctgt
      421 ttgtaggttt gttggtgcag taggagcaga gtttgaatgc attttaaagt tttgtactgc
      481 tgaccaaggg ctaactaacc ccccattatc ccagaaacgt attctccaat aatatgttgt
      541 tcctgaccat cctaaggaag agccatcata atatatattg gggcttcttg acccaattgc
      601 agttgctgtc atactttgtg atcctgaatc ccacattact gtgccattaa atcctgatgc
      661 tgtatttact tctatttcat aataattacc tgtatttcct gtatcaggat cattaaagat
      721 agcactaaaa tatggtgttt gtgttgtaac atttgttgga ttagtagttc ccatacagta
      781 tggtgtagta ggagcagtag gggcttgatt agcaatcacc cattgtgcgg taattgaaaa
      841 agcacccaca acactactac atacaccagt agatgagtta aaggttcccc cacagtttcc
      901 agaggggctt attgtaaatc ctgtaaaatt atatccactt cttgtgcaag taactactcc
      961 agatgcggaa ccatgagata tgctctgtgt agatggagag catgttcctc catttcctaa
     1021 tgctgatatt gtgtaataat taatagtcca atttggttgg ataacaagag gtcctgtaac
     1081 tttactacat acacctgttg aggaattaaa tgttcctcca catgatccag aataaataga
     1141 ataaccactt actgtatatc ctgttcttgt acaacttcct gctgctggtg ctggagatgg
     1201 actatcgcca catacttcag acggacttaa tggactacaa gttccaccat tacctgtaat
     1261 tgatacacta tatttttttc tgtagcatgt aatcgatgcg gttccgcaag atccaataca
     1321 atcatatttt gtacaggaaa aactagctgc actacagcca ttatcactag attggtaatt
     1381 agcatcacat actggtgtac aactctcaca ataaccccta tcacaacaca ttgtacctgc
     1441 ctcaccacaa ttcagactag cttcatattg attgcatgtt cccctataaa cacaaccacc
     1501 atctccaaca caaccacaat acttatgctg ataacagtca gatcgacatt ctgcattcac
     1561 atctgatttt gttagtagga atattgatat caaaagaaaa gataaaagaa taatacaaaa
     1621 caagttttta cttatcccct ttattacgag tttattattt a
//
LOCUS       DAOSAB010000133         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_49963_length_1651_cov_2.447368, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000133 DAOSAB010000000
VERSION     DAOSAB010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49963_length_1651_cov_2.447368"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PK432_02605"
     CDS             <1..499
                     /locus_tag="PK432_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK59849.1"
                     /translation="ILVDVLTDNRNRTVAELRRLFEEIGGRLAENGSVSWNFDTKGLI
                     ILRSGHMEKAEKFGAEDKYVKENREEVMMKLMDIKGVEDIKEVDLDGIEGIEVYTKFT
                     DLAHVRDEIEKLGYVIEEADIIKEPKVTKQLSGSDFVRAQEGLEKLDDHNDVQNVWSN
                     LEEVN"
     gene            512..1003
                     /gene="ruvC"
                     /locus_tag="PK432_02610"
     CDS             512..1003
                     /gene="ruvC"
                     /locus_tag="PK432_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOK59850.1"
                     /translation="MRILGIDPGIAITGWAVVDFNDNGGATPIDYGAITTSKQALIGE
                     RLLELYNDLREIIKEFNPEYCGLETLLFYNNAKTAIVVGEARGVVLLVLEELHIPVHE
                     FTPLQVKSSISGYGKADKRQVEENVKRLLQLDEIPKPDDVSDALAISIACFDRLKMDN
                     LLK"
     gene            1019..1603
                     /gene="ruvA"
                     /locus_tag="PK432_02615"
     CDS             1019..1603
                     /gene="ruvA"
                     /locus_tag="PK432_02615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOK59851.1"
                     /translation="MISYISGKVLKNNIGKNNYVDVLLPSGIAYRVFVTSMFNFVQVG
                     SQISVYTSFQVREDSQTLYGFATQEERDFFEELLTVSGIGPKSALSILSTYSMDRVKK
                     IILDGDSVLLSKTPGLGLKGAQKVILELRGRIDFEKDDLQMKENVKLGELKDALKSLG
                     FAGKDLKDGIKRGEEVLKEGDVDIEILIKKVLQD"
BASE COUNT          604 a          170 c          386 g          491 t
ORIGIN      
        1 aatacttgtt gatgttttaa ctgataacag aaacaggacg gttgctgaac tgcgaagatt
       61 gtttgaagaa atagggggga gattagcaga aaatgggtct gtgtcatgga attttgatac
      121 taagggttta attattttaa gaagtggaca tatggaaaag gcagaaaagt ttggagcaga
      181 agataaatat gttaaagaaa atagagaaga ggtaatgatg aaattaatgg atattaaagg
      241 tgtagaggat ataaaggagg tggatttaga tggtatagag ggaatagagg tttatacaaa
      301 gtttactgat ttagcacatg tcagggatga aatagaaaaa cttggctatg ttatagagga
      361 agcagatatt attaaagaac caaaagtgac taaacagtta tcaggaagtg attttgtaag
      421 agcacaggaa ggccttgaaa aacttgatga ccataatgat gttcaaaatg tatggagcaa
      481 cctagaagaa gtaaattaat gagagtgtaa tatgagaata ttaggaatag atccgggcat
      541 agcaattacg ggatgggctg tcgttgattt taatgataat ggtggtgcta cccctataga
      601 ttatggggca ataactactt ctaaacaagc acttataggt gaaagactcc ttgagctata
      661 taatgatttg agagaaatta ttaaagaatt taatcctgag tattgtggtt tagaaactct
      721 tctcttttat aataatgcta aaacagcaat agttgttgga gaggctaggg gggttgtgtt
      781 acttgtgcta gaagagttac atatccctgt gcatgagttt acccctttgc aagtaaaaag
      841 cagtatttca ggatatggga aggcagataa aaggcaggtg gaggagaatg ttaaaaggtt
      901 attacagctt gatgagatac ctaaacctga tgatgtttca gatgctcttg ccatttctat
      961 tgcctgtttt gatagattaa agatggacaa tttacttaaa tagtttaaaa ttcttaatat
     1021 gatttcatat atatcaggga aagtattaaa aaataatata gggaagaata actatgttga
     1081 tgttctttta ccatcaggga tagcatacag ggtttttgtt acatcaatgt ttaattttgt
     1141 gcaggtgggc tcacagatat ctgtatatac aagttttcaa gttagagaag atagccagac
     1201 tttgtatggt tttgctactc aagaggagag agatttcttt gaagagcttt taactgtttc
     1261 aggaatagga ccgaaatctg cattgtctat actttcaaca tacagtatgg atagagttaa
     1321 gaagattatt ttagatggtg attctgtact tttaagcaaa acaccaggac ttggtcttaa
     1381 gggtgcacaa aaagtcatac ttgagttgag aggaaggata gattttgaaa aagatgattt
     1441 gcaaatgaag gagaatgtaa agctaggtga gctaaaagac gcattaaaat cactaggttt
     1501 tgctggtaaa gacttaaaag acggtataaa gagaggagaa gaggttctta aagagggaga
     1561 tgtagatatt gaaatattaa taaaaaaagt tttacaggat taaacatatg attaaatcaa
     1621 gtaaaaaaac tgaagaaaaa aatatgctta a
//
LOCUS       DAOSAB010000134         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_49972_length_1651_cov_2.136591, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000134 DAOSAB010000000
VERSION     DAOSAB010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49972_length_1651_cov_2.136591"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..570
                     /locus_tag="PK432_02620"
     CDS             229..570
                     /locus_tag="PK432_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59852.1"
                     /translation="MKNIQHKQQASGEWFKKSFLEQMANIGSEVYRAINWRNKGDEEY
                     AYEAFVRSLELFDLTKESKLTYPQLKELNRVRELWVDYFMYDNVYNSTAEGINKYFMD
                     VTIAFKNKLSN"
     gene            758..1033
                     /locus_tag="PK432_02625"
     CDS             758..1033
                     /locus_tag="PK432_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59853.1"
                     /translation="MEKIKKYTTTEFYNNPSKALSHVMNGGVVYLGYKRVKEPLAVLI
                     PYKEYVLGKKRTAKKGKSLWDRYGNKAMHAPEYKDSVGYIRKQRDSE"
     gene            1030..1419
                     /locus_tag="PK432_02630"
     CDS             1030..1419
                     /locus_tag="PK432_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOK59854.1"
                     /translation="MTSYLLDTSFISAFCNKDDANHKRAQEISKEIKDGYFIIPSVVV
                     AELSSFYKNLDFRNLVVETSLDIASEIASVTSENILIYVSFVQEFFCNLKAVDSMILF
                     PSIENDAKLLTFDKRLEKEFTEVSKKI"
     gene            1479..>1651
                     /locus_tag="PK432_02635"
     CDS             1479..>1651
                     /locus_tag="PK432_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK59855.1"
                     /translation="MYNFKRKYEEPIKKDFFKGKIIIVTGARQVGKTTLVEHILQEYD
                     NVVYLNADNPKDR"
BASE COUNT          643 a          165 c          318 g          525 t
ORIGIN      
        1 taaatgtagt gctgtagtta tttcaactgc tcatagggta tttggtgatt taacgcaaga
       61 gatttgtaag agagataata ttttggttgt tatagatggt atgaataaac tagataaaga
      121 gaaaataatt aaatcaggaa agatatatag agggataggg agataagtta tggttaaaat
      181 atagtagaag aaaataacag atattgttaa ttctaagata tcttttgaat gaaaaatatt
      241 cagcataaac aacaagcttc tggagaatgg tttaagaaat catttcttga acaaatggct
      301 aatattggta gtgaggtata cagggctatt aattggagaa acaagggtga tgaagagtat
      361 gcttatgagg catttgtaag gtctttagaa ctctttgatt taactaaaga atctaaatta
      421 acatatcctc agcttaaaga attaaacagg gtaagagaat tatgggtaga ttattttatg
      481 tatgataatg tatataattc aacagcagaa gggataaata aatactttat ggatgtaaca
      541 attgctttta aaaataaatt aagcaattaa gtatttctaa tatgttatta atagctaagt
      601 agacaaatta tgcctaattt aaggaattaa ccgtacaaat taggcataaa ataaggagtt
      661 gaaatttcag taaagatatt aatatccata taacctaaaa agagcctata taaagtttgc
      721 attgatgagg ttggtaactt tttggtaata tgttggtatg gaaaaaataa aaaagtatac
      781 aacaacagag ttttataaca atccttctaa agcacttagt catgtaatga atggaggagt
      841 ggtctattta ggctataaaa gagtaaagga gccgttagca gtattaattc catataagga
      901 gtatgtatta ggaaagaaga ggactgctaa aaaagggaaa tctttatggg ataggtatgg
      961 aaataaagct atgcatgctc cagagtataa agatagtgtt ggttacatta gaaagcagag
     1021 ggatagtgaa tgacaagtta tcttcttgat acaagcttta taagtgcttt ttgcaacaaa
     1081 gatgatgcta atcacaaaag ggcacaagag atttctaaag aaataaaaga tgggtatttt
     1141 ataattcctt ctgttgttgt agcagagtta tcatcttttt ataaaaattt agattttaga
     1201 aatcttgtag tagaaactag cttggatatt gcatctgaga tagcttcagt aacaagtgaa
     1261 aacattttaa tatatgtcag ttttgttcag gagtttttct gtaatctcaa ggcagtagat
     1321 tctatgattt tgtttccttc aattgaaaat gatgcaaagc ttttaacatt tgataaaaga
     1381 ctggaaaaag agtttacaga agtttctaag aagatttgac aactccataa attaggcata
     1441 aaatagggag tacattcctt aaattatgcc taattattat gtataacttt aaaagaaaat
     1501 atgaagaacc cattaaaaag gactttttta aagggaaaat aattattgta actggtgcaa
     1561 gacaagttgg taaaacaaca ctggtagagc atattctgca agaatatgat aatgttgtat
     1621 accttaatgc tgataatcca aaagacagat t
//
LOCUS       DAOSAB010000135         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_50339_length_1642_cov_3.045999, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000135 DAOSAB010000000
VERSION     DAOSAB010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50339_length_1642_cov_3.045999"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="PK432_02640"
     CDS             <1..1159
                     /locus_tag="PK432_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOK59856.1"
                     /translation="SHILNGYIPVYESTVTQKLLNEGAIILGKTNLDQFCHGSSTITS
                     YYGPTRNPHDLTRLPGGSSGGSAAATAADMCAGSIGTETAGSIRQPSSWCGTVGLKPT
                     YGRVSRYGVLAMGSSLDCPGPITKDINDSAYLLSIIAGYDKHDFSSYDIPVLSYYKNI
                     DTSKIKGMKICIPNEYLDMEIQKEVMENFLVSVDILKRLGAEVEYVNVLNPKYAMSVY
                     TITCRSEVSSNLARYDGTRYGLEGSDKSTVTAYYESTRGEGFGQEPKRRVMTGTYSLS
                     AGYADEYFKKSEQVREVIREDLDNILKKYDCIVSPATPTVALKDSDADNPLFGEMSDI
                     LAEGSSEAGLPAISIPNGTAKNLPTGIQFIGRSFDEQTIINIAKAVEQTFS"
     gene            complement(1156..>1642)
                     /locus_tag="PK432_02645"
     CDS             complement(1156..>1642)
                     /locus_tag="PK432_02645"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOK59857.1"
                     /translation="FDKANPSGVSDGYDYASLTVNLKPYKPVEEPQSLETKIFFANVK
                     LNEETSDCKIVYPVSRSLQNVAGIERLAVEELIKGPTEEEKAEGYISEINPNVKVLGL
                     SIEDGVARVDFSKELEEGVAGSCKVESIRAQIEETLKQFDNIDSVIISVEGEVEGILQ
                     P"
BASE COUNT          536 a          293 c          294 g          519 t
ORIGIN      
        1 ttcacatata ctaaacgggt atattcctgt atatgaatct actgtaacac aaaaactatt
       61 aaatgagggt gcaattattc ttggaaaaac aaatttagat caattttgtc atggttcttc
      121 tacaattacc tcatactacg gtcctacaag aaatccacat gacttaacaa ggcttcctgg
      181 aggctccagc gggggctctg ctgctgctac tgctgcagat atgtgtgcag gctctattgg
      241 aacagaaaca gctggttcta ttcgtcagcc ttctagctgg tgcggaacag taggtcttaa
      301 acctacatat ggaagagtat caagatatgg tgtacttgca atgggatctt ctcttgattg
      361 tcctggtcca attactaagg atataaatga tagtgcatat cttttatcaa taattgctgg
      421 atatgataaa catgattttt cttcatatga tattcctgtt ttaagctact acaagaatat
      481 tgatacttca aagataaaag gaatgaagat atgtatccct aacgaatatc ttgatatgga
      541 gattcaaaaa gaggtaatgg agaattttct tgtttctgta gatatcctaa aaagattagg
      601 agcagaagtt gagtatgtaa atgtacttaa tcctaaatat gctatgtctg tatataccat
      661 tacatgcagg agtgaagtct cttccaatct tgcaagatat gatggtacta gatatggact
      721 tgaaggtagt gataaatcaa cagtaactgc atattatgaa tcaacgagag gagaagggtt
      781 tggacaagaa cctaaaagaa gggtaatgac aggtacctac tctctttctg ctggatatgc
      841 tgatgaatat tttaaaaaat cagaacaggt gagagaagtg ataagggagg atttagacaa
      901 tatattaaaa aagtatgatt gtatagtatc accagcaaca cctactgttg cactaaaaga
      961 tagtgatgct gataacccac tctttggtga aatgtcagac attcttgcag agggaagctc
     1021 agaagcagga ttacctgcaa tatctattcc caatggaaca gcaaaaaacc ttccaacagg
     1081 aattcagttt attggaagat catttgacga acagacaata attaatattg cgaaagcagt
     1141 tgaacaaact ttttcttaag gctgaagaat tccctctact tccccttcta ctgagataat
     1201 tacggaatca atattatcaa actgttttaa tgtttcctct atttgagctc tgattgattc
     1261 aaccttacaa cttcctgcaa caccctcctc taactcttta ctaaaatcaa ctcttgctac
     1321 tccatcttca attgaaagtc ctaaaacttt tacattagga ttaatttctg aaatatatcc
     1381 ttccgctttc tcttcctctg tagggccttt aattaattct tctactgcta acctttcaat
     1441 accagcgaca ttttgtaaag atcttgatac aggataaact attttacagt ccgaagtttc
     1501 ttcattcaat ttaacattag caaaaaagat tttggtctct agagattgtg gctcctctac
     1561 tggtttgtaa ggctttaaat taactgttag agaggcataa tcatagccat cagaaactcc
     1621 agaagggtta gctttgtcaa at
//
LOCUS       DAOSAB010000136         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_50878_length_1630_cov_3.178413, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000136 DAOSAB010000000
VERSION     DAOSAB010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50878_length_1630_cov_3.178413"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1630)
                     /locus_tag="PK432_02650"
     CDS             complement(<1..>1630)
                     /locus_tag="PK432_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59858.1"
                     /translation="KSAAGVSVSEIVEAEKAVSKLYEENNEFRKAFDELKDLQDHKNN
                     YDSVIQNDIIDIMTSILELPGKAISLLSPNATNLVEPVADELKEKISEADGETDYSKY
                     TLTGKSIKGISPTSMYREDYNNKRQQENISGKGSLGITAIENYMNNLLNMVGLTMNKT
                     VSLSITRKGKKNAQVIHEIDVPLAHNRLNGKISLTHIMDAAKENSIADIIAQLMNGFV
                     DVGKKAWVAYIQGNMEVIPKILFMIEIGVPFKDIAYFVSNPITREYVKEKKKKMSIHS
                     TLMYDESFSSSFDAAVKTKTEIFNNIDLSKLSNDALLNIEGANANIFGYDAASKELAG
                     KNTFTREALKKVAESKLNYKNKDQVAGFMQYLYIENLIEDYDLVKRAMNPDTKETSDF
                     YGDDARIEEVKKTQELKSIDTEPINKLRTNSLISPLFIQEFAKKLFGRVFRLRNDERI
                     NKYLRDLYSHKFKRSGPKKVTGYDMETYITKFKNFLGQYLFANELRQYNSALGTFKAK
                     TTDKAEIVDKAVLEELNQEYDYISSLTPKDEFVQN"
BASE COUNT          488 a          294 c          213 g          635 t
ORIGIN      
        1 tattttgaac aaactcatct tttggagtaa gactactaat atagtcatac tcttgattta
       61 attcttcaag aacagcttta tctacaattt ctgccttatc tgtagttttt gccttaaatg
      121 ttcctagtgc tgagttatat tgtcttaatt cattagcaaa taaatattgg ccaaggaagt
      181 tcttaaactt agtgatataa gtttccatat catatcctgt aactttctta ggacctgatc
      241 ttttaaactt atgactgtat agatctctca agtacttgtt tattctttca tcatttcgta
      301 gtctgaatac tctaccaaaa agttttttag caaactcttg tataaataat ggtgatatta
      361 aactattggt tcttagttta ttaataggtt cagtatcaat actttttagc tcttgagttt
      421 tctttacttc ttcaattctt gcatcatcgc catagaaatc actggtttct ttagtatcag
      481 gattcatagc tcttttaaca agatcataat cctctattaa gttctctatg taaaggtatt
      541 gcataaagcc agcaacctga tctttgtttt tataattaag cttagattca gccactttct
      601 taagagcttc tctggtaaaa gtatttttac cggctaactc tttactagct gcatcataac
      661 caaatatgtt agcattagca ccctcaatat taagaagggc atcattagat aactttgata
      721 agtctatatt attaaagatt tcagttttgg tttttactgc tgcatcaaaa ctactactaa
      781 atgattcatc atacattaaa gtagaatgta tagacatctt ctttttcttc tccttaacat
      841 attctcttgt aataggattt gaaacaaagt atgcaatatc tttaaatggt actccgatct
      901 ctatcataaa taagatctta ggtataactt ccatgttacc ttgtatatag gcaacccaag
      961 cttttttacc tacatccaca aaaccattca ttaactgagc tataatatct gctatagagt
     1021 tttccttagc agcatccatt atatgagtaa gtgatatctt accattcaat ctgttatgag
     1081 ccaatggaac atcaatctca tgaattactt gtgcattttt cttacctttc cttgttatag
     1141 ataaacttac tgttttattc atagtaagtc caaccatgtt aagaaggtta ttcatgtagt
     1201 tctctatagc agtaatacct aaagaacctt taccagagat attctcttgt tgtctcttgt
     1261 tattgtaatc ctccctatac atagaggtag gacttatacc ttttatagac ttgcctgtta
     1321 aggtatactt agagtaatca gtttcgccat cagcttctga tatcttttct tttaattcat
     1381 ctgccacagg ttcaacaaga tttgtagcat taggtgaaag taaagatata gccttaccag
     1441 gtagttctag aatactagtc atgatatcaa taatatcatt ctgtataaca gaatcatagt
     1501 tatttttatg atcctgtaaa tcttttaact catcaaaagc ttttctgaat tcattgtttt
     1561 cttcatatag ttttgataca gctttctctg cttcaactat ttcagataca ctaactcctg
     1621 ctgcagattt
//
LOCUS       DAOSAB010000137         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_51096_length_1626_cov_2.052833, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000137 DAOSAB010000000
VERSION     DAOSAB010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51096_length_1626_cov_2.052833"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1626
                     /locus_tag="PK432_02655"
     CDS             39..>1626
                     /locus_tag="PK432_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59859.1"
                     /translation="MAESENTSNQESRTFDKSLNRDINDFPLPPNQWTRARNAINNSI
                     TGDLGKLGNEPANLLCVTAPYTIIGFIHIIEDKWAVYSTDGTNSEIGLFTESDCGTSK
                     AAYVPIVNAACLNFKVENLIIGVSRQTSTCTYKLYWDDGLNPSRNLEIDVDNPVNNYP
                     FLPPPAAPGTTNPNTPIPYIQNCVDSNGPAPGGCIICTNTPVLDCDKLRVALFIDPIC
                     PRVEKGIGGGNLLNGSYFVVMAYAIKGQKIGDWYVSNVQGLFDHNNSATSLDVFIDRI
                     DPDYDEIIIGLGSVVNAQTVVRQAGIYSSRQTLFNFDTIFDQWPAIPIEQLPLMTPIV
                     NKSDAMYNVGDYLIRVGPTSKQDFNYQPLANQITAKWQAVEYPADYYHKGNNQTGYMR
                     DEVYPFVIQWIYDTGDKSAEYHIPGRPPVSILPDKNPVAPGGNVFAGETEYWQVYNTA
                     TYTAVSIPNGDGGTIVAEGLMGYWESTEFYPDKKPQIWNSSAHPWSAITSLPYANTTF
                     PDYNLCGTPIRHHKFPEDTVG"
BASE COUNT          520 a          270 c          307 g          529 t
ORIGIN      
        1 tatcccaata atgttaatgg cttaatgaat ggatactaat ggcagaatca gaaaatacta
       61 gcaatcaaga atcaagaaca tttgataaat ctttaaatag agatattaat gactttccct
      121 tgccacctaa tcagtggacc agggctagaa atgctatcaa taactctata accggtgacc
      181 taggaaagtt aggtaatgag ccggcaaatc tcttatgtgt aacagctcct tatacaatta
      241 ttggattcat tcatattatt gaagataagt gggcagttta ttctactgat ggaactaatt
      301 cagaaattgg cctatttaca gaaagcgatt gtggaacttc taaagctgct tatgtaccta
      361 tagtaaatgc tgcatgcttg aatttcaaag ttgaaaactt gatcataggt gtatctcgtc
      421 aaacaagcac atgtacttat aaactttact gggatgatgg tttaaatcct tcaagaaatc
      481 ttgaaattga tgtagataat cctgtaaata attatccttt tttaccacct cccgcagcgc
      541 caggaactac taatcctaat acacctattc cttatattca gaattgtgta gatagtaatg
      601 gcccggcacc gggtggttgt attatctgta ctaatactcc agttttagat tgtgataaac
      661 taagagtagc tttgtttatt gatcctattt gtcctagagt agagaaaggt attggtggag
      721 gtaatctttt aaatggatca tactttgttg taatggctta tgctattaaa ggtcagaaga
      781 ttggtgactg gtatgtatct aatgttcaag gattatttga tcataataat tcagctacat
      841 cattagatgt atttattgac aggattgatc ctgattatga tgaaataatc attggtctag
      901 gaagtgtagt taatgctcag acggttgtta gacaagctgg tatctattct tcaagacaaa
      961 ctttatttaa ctttgatacc atatttgatc agtggccggc tattcctata gagcagttac
     1021 ctttaatgac tcctatagtt aataagtcag atgccatgta taatgttggt gattatctta
     1081 ttcgtgtagg tcccacatca aaacaagatt tcaactatca gcctttggca aatcagatca
     1141 ccgctaagtg gcaagctgta gaatatcctg cagattatta tcataaaggt aataatcaaa
     1201 ctgggtatat gcgtgatgaa gtttatcctt tcgttataca atggatatat gatacaggag
     1261 ataagtctgc agaatatcat attccaggta gacctccagt aagtatttta ccagataaaa
     1321 atccagtagc tcctggagga aatgtatttg ctggggaaac tgaatattgg caagtatata
     1381 atacagctac ctatacagct gtatccatcc ctaatggaga tggaggaaca attgttgctg
     1441 aaggtcttat gggatattgg gaatctactg aattctaccc tgataaaaaa cctcagatat
     1501 ggaactctag tgcacatcct tggtctgcca taacaagttt accttatgct aatacaacat
     1561 ttcctgatta taatctttgt ggtactccaa taaggcatca taagtttcct gaagatactg
     1621 ttggac
//
LOCUS       DAOSAB010000138         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_52033_length_1605_cov_3.376774, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000138 DAOSAB010000000
VERSION     DAOSAB010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52033_length_1605_cov_3.376774"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PK432_02660"
     CDS             complement(<1..379)
                     /locus_tag="PK432_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOK59860.1"
                     /translation="MRTEEENREIAQLLFPDVAESVEDIFKRYPKRKLKDSQLVVRFA
                     PSPTGFLHLGSVYTSLINRKLADQTNGICILRIEDTDKKREIKGGVDLIINGLEKFGI
                     KFDESVKKGEYGPYIQSERREIYK"
     gene            442..>1605
                     /locus_tag="PK432_02665"
     CDS             442..>1605
                     /locus_tag="PK432_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59861.1"
                     /translation="MTLTEAAFWSKRLGVIVIGIFAFILLVTLIAYNPGNVELPPQYL
                     TPNCACTETADEFLKHALTIPSYEISPNSTPQYAIQTETGVLDDNLPDIVNVYKYTDL
                     GEKINAQSLAKVLAGKMGFKAEEIRTLGTKEYIWQDFETQRALRVTVEDLNFTLTTNI
                     SKIRSARAAADLPSEEEAKRIAKNALTELGVYFSGYNDGSLLTYLININEDGSYTEAD
                     SLLNAELIRVDFYKTFPLISIPSNIVGADRMRASLENRNMAAIVDQMTINDKTIEVYN
                     FKTFVTHQNPNKSNVSVYVGPESEDFKKLKNIYQIDYKSWKIEPESCGTYPLISAAVA
                     EEKIKNGEGSLVSLDKNGDEVAQYTPQEVKQFLITNITITYYEGSIVQHYLQPV"
BASE COUNT          581 a          266 c          257 g          501 t
ORIGIN      
        1 ctttgtatat ctctctcctt tcactctgaa tatatggacc atactctcct ttcttaacac
       61 tttcatcaaa tttaatacca aacttttcta aaccgttaat aataaggtca acacctcctt
      121 ttatctctct ctttttatca gtatcttcta tcctaagtat acaaataccg ttagtttgat
      181 cagctagttt acgatttatt aatgatgtat atacagatcc tagatgtaaa aaaccagtag
      241 gacttggagc aaaccttaca acaagctgag aatcttttaa ctttctctta ggatatcttt
      301 taaatatatc ctcaacactt tccgctacat ctggaaaaag taattgtgct atctctctgt
      361 tttcttcttc tgttctcatc taaaagttgt gatacaattt aatatattga tatatctaat
      421 atacattgtt atcattatac catgacactt acagaggcag cattttggag taaaagatta
      481 ggggtaatag taattggcat ttttgctttt atccttttag taactttaat agcttataac
      541 ccagggaatg tagaacttcc tccacagtac ttaactccta actgtgcatg tacagaaaca
      601 gcagatgagt ttttgaaaca tgcattaaca ataccttcct atgaaatatc tccaaactct
      661 actcctcagt atgcgattca aactgaaaca ggagtgcttg atgataatct ccctgatatc
      721 gtaaatgtat acaagtatac agacttggga gaaaagataa atgcacagtc tttagcaaag
      781 gtacttgcag gaaaaatggg gtttaaagct gaagagataa ggactctggg aactaaagaa
      841 tatatatggc aggattttga aacacaaagg gcacttagag taacagttga agatttaaac
      901 tttaccttaa ctacaaatat atcaaaaata aggtcagcaa gagctgctgc agatctgcca
      961 agtgaagaag aagcaaagag gattgctaaa aatgctctaa ctgagcttgg tgtctatttt
     1021 agtggttata atgatggatc cttacttaca tatttaataa atataaatga agatggttca
     1081 tatacagaag cagattcttt actcaatgca gaattaatta gagtagattt ttataaaaca
     1141 tttcccttaa tttcaatccc aagtaatata gttggtgcag atagaatgag agcttctctt
     1201 gaaaatagga atatggctgc aattgttgat caaatgacaa taaatgataa aacaatagaa
     1261 gtgtataact ttaagacatt tgtaacacac caaaatccta acaaatcaaa tgtatcagta
     1321 tatgtaggac cagaaagtga agattttaaa aagttaaaaa atatatacca aattgattac
     1381 aaatcatgga aaatagaacc tgaaagctgt gggacatatc ctttaattag tgccgcagtt
     1441 gctgaagaaa aaataaaaaa tggagagggc agtcttgtat ctttagacaa gaatggagat
     1501 gaggtagctc aatatacacc acaagaagta aaacagtttc ttatcacaaa cataacaata
     1561 acatattatg aaggatccat tgtgcaacat taccttcagc cggta
//
LOCUS       DAOSAB010000139         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_53075_length_1584_cov_3.519294, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000139 DAOSAB010000000
VERSION     DAOSAB010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53075_length_1584_cov_3.519294"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..497)
                     /locus_tag="PK432_02670"
     CDS             complement(294..497)
                     /locus_tag="PK432_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59862.1"
                     /translation="MPLNVGLILVGMFLLIVIVLLDRLMNVKNIQSKISEGVYMFIVQ
                     LEMSICAFALLMTAVGIIWLIIE"
     gene            complement(582..1079)
                     /locus_tag="PK432_02675"
     CDS             complement(582..1079)
                     /locus_tag="PK432_02675"
                     /inference="COORDINATES: protein motif:HMM:NF039500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HOK59863.1"
                     /translation="MSRIKLIWTTMISREDLKKLLREFNEKGYSDPNSKYIDNGKNGK
                     VLKLNIGEYSYEDEYYGGEPYCGNETIWEKGIDIFRCVYWGKVVDGVDFGSIYGFLRE
                     ALSKGPDGELVHRGPTEYISENLKYTNEIVGDIYEFRQVERIYLNGTEVYTAYFIGGR
                     INVRK"
     gene            complement(1072..>1584)
                     /locus_tag="PK432_02680"
     CDS             complement(1072..>1584)
                     /locus_tag="PK432_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HOK59864.1"
                     /translation="IMISAFTRLLLYEDAYGFTTLRLYSHVFIIFLTIVFGLLLYKVL
                     KNGKDSSLSLGVFLSVILFLVGMNILNPDAFIAQKNIQRFNDGKKLDVYYLTTLSDDA
                     VPVTIKMLDISDESVKGEFAYYMYWKMQGRIPSNNWQSFNVSHKRAKEIIAPKMEVLE
                     EYKEYTEEFE"
BASE COUNT          560 a          347 c          152 g          525 t
ORIGIN      
        1 atacagagac aataataaga aaagagttat cttttttctt ctgtggtttc tgctcatcat
       61 tattagagac ctcctctggg gcatcaacct gctcaataga agatataaaa tctgataatg
      121 gtctttgatt actttccata atgctctatt aataatttat ttacttttta cagtataaac
      181 aaaaaatatt aaaattacaa acacccaata attatgaaaa aagcctagaa atattaagga
      241 taaagagcaa aagctcatat atattcctaa ttctaggctt ctcctgcact tatttattct
      301 attattaacc atattatccc cactgctgtc atcaaaagag caaaagcaca aatactcatt
      361 tccaactgaa ctataaacat ataaacccct tctgaaatct tactttgaat gttctttaca
      421 ttcatcaatc tatccaaaag aacaatcaca ataagcaaaa acatacctac taatattaaa
      481 ccaacattta acggcatatt ttttctcctt tattcttcta tttttcaagt gcagggaaca
      541 tatgtattat acaacatatc aacagaatct tttaacaata gctatttcct tacatttatc
      601 ctacccccta taaaatatgc agtatatacc tcagtaccat ttaaatatat tctttccact
      661 tgcctaaatt catatatatc ccccactatt tcatttgtat acttaagatt ttcactaata
      721 tattcagtag ggcccctatg taccaattca ccatcaggac ctttagataa agcttctctt
      781 aagaaaccat atatgctacc aaaatcaaca ccatctacaa ccttacccca gtatacacat
      841 ctaaatatgt ctatcccttt ctcccatatt gtttcattcc cacaatacgg ctctcctcca
      901 tagtattcat cttcatatga atattcccca atattaagct tgagtacctt cccatttttg
      961 ccattatcaa tatacttaga attaggatct gaatacccct tctcattaaa ctctcttaat
     1021 agcttcttca aatcttccct tgatatcatg gtagtccaaa ttaacttaat tctactcaaa
     1081 ctcctctgta tattctttat actcttctag cacctccatt ttaggagcaa ttatctcttt
     1141 tgccctcttg tgagaaacat tgaaagactg ccagttattt gaaggtattc ttccttgcat
     1201 cttccagtac atataatatg caaactcccc ctttacactc tcatcagata tatctaacat
     1261 cttaatcgta acaggaacag catcatcaga taaagtagtt aagtagtaaa catcaagctt
     1321 ttttccatca ttaaacctct gtatattctt ctgtgcaata aaagcatcag gatttaatat
     1381 attcattcca acaagaaaaa gaataacaga aagaaacact cctaaactta aactgctgtc
     1441 tttcccattc ttcaatactt tgtaaagaag caaaccaaaa acaatggtaa gaaagataat
     1501 aaaaacatga ctatacaacc tcaaagtagt aaaaccatat gcatcttcat aaagcaaaag
     1561 acgagtaaat gcagatatca ttat
//
LOCUS       DAOSAB010000140         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_53229_length_1581_cov_2.749672, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000140 DAOSAB010000000
VERSION     DAOSAB010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53229_length_1581_cov_2.749672"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK432_02685"
     CDS             <1..399
                     /locus_tag="PK432_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59865.1"
                     /translation="IKKVVSYFDENERVLLNDLGWNMLPNVYRGHNQWSAEWKLEFSD
                     WVPRKKYLKNFNTMNTFSMFGKSIESIKSFVDFADVGKIGLVVSNLEEYTFPYQDMLE
                     EFKKTEWLELDEVVSLKNKTMYIFDVIEYE"
     gene            392..1336
                     /locus_tag="PK432_02690"
     CDS             392..1336
                     /locus_tag="PK432_02690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK59866.1"
                     /translation="MNRSKNIKNSPLVSVIIPTYNGESTILRAINSVLNQTYSNLELI
                     VVDDCSKDNTFEVVKNVKDKRVKVLRHKENRGGAAARNTGIKEAKGEYIAFLDDDDEW
                     LSEKVEKQVEYLKSKGSSLYKGVVCSHMILSGKKWRTVIQTKEGDLREDIFLMRLSLA
                     AGSCLMVSRYVFNDIGMFNESYLRHQDMEFVLRYLRKYKLGVMKEPLAKIYGHSGNVS
                     GEKMQEVKSKFLKDFEKDINSFDKKTAKKIYARQWLQVSKHYALDGDIKNTFKYLHTS
                     LSYGILFSNRYKFIILENYIALPYHLLKGLVERKRYER"
     gene            1326..>1581
                     /locus_tag="PK432_02695"
     CDS             1326..>1581
                     /locus_tag="PK432_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59867.1"
                     /translation="MKDRKRILIIAGCTDKKLISKISPILDSRYVEEVYLVRNTEMQY
                     SHPKLVQYSVFKLFRKILPLRELNRIISSIYILLFKRIDII"
BASE COUNT          596 a          158 c          324 g          503 t
ORIGIN      
        1 attaaaaagg tagtatctta ctttgatgaa aatgaaagag ttcttcttaa tgatctaggg
       61 tggaatatgc ttcctaatgt ttacagagga cataaccagt ggagtgcaga gtggaagtta
      121 gagtttagtg actgggttcc tagaaagaag tatttaaaga attttaatac tatgaataca
      181 tttagtatgt ttggaaagag tattgagagt attaaaagct ttgttgattt tgctgatgta
      241 ggaaagattg gtttagttgt atctaattta gaggaatata cttttccata tcaggatatg
      301 cttgaagagt ttaaaaaaac agagtggtta gaattagatg aggtagttag tttgaaaaac
      361 aaaacaatgt atatttttga tgtaattgag tatgaataga agtaaaaaca ttaaaaattc
      421 tccattagtg tctgtaatta ttcctacata caatggggaa agtactatac ttcgtgcaat
      481 taatagtgta cttaatcaaa catattctaa tcttgagtta attgttgtag atgattgttc
      541 taaggataat acatttgaag ttgtaaaaaa tgtaaaagat aaaagggtaa aagttttaag
      601 acataaagag aatagagggg gagctgctgc aaggaacaca ggaataaagg aggcaaaggg
      661 agagtatata gcattcttag atgatgatga cgaatggctg agtgaaaaag ttgaaaaaca
      721 ggttgagtac ttaaagagta aaggatcttc gttgtacaaa ggggtagtct gtagccatat
      781 gattttgtct ggtaagaaat ggaggacagt tatacagaca aaagagggtg atttaagaga
      841 agacattttc cttatgcggc tctctcttgc cgcaggatca tgtttaatgg ttagcaggta
      901 tgtatttaat gatattggta tgtttaatga aagttatctt aggcatcagg atatggagtt
      961 tgtattaaga tatttaagga agtataaact gggtgtaatg aaagagcctt tggcaaaaat
     1021 atatggtcat tctgggaatg taagcggtga gaaaatgcaa gaggtaaaaa gtaaatttct
     1081 taaagatttt gagaaagata ttaatagctt tgataagaaa acagcaaaaa aaatatatgc
     1141 aagacaatgg ctacaagtat caaagcatta tgcactagat ggggatatta agaatacatt
     1201 taagtatctt cacacctctt tatcatatgg aatacttttc tcaaatagat acaaatttat
     1261 catattagaa aactatattg cccttcctta tcatttatta aaaggtttag ttgaaagaaa
     1321 aagatatgaa agatagaaaa aggattttaa taattgcagg atgtacagat aaaaagctta
     1381 tatcaaaaat ctcccctata cttgatagca ggtatgtaga agaagtatat cttgttagaa
     1441 acacagaaat gcaatattct catcctaagt tagtgcagta ttctgtgttt aaactattta
     1501 gaaaaatact tccacttaga gaattaaaca gaataattag tagtatatac attcttttgt
     1561 ttaaaagaat tgatattatt c
//
LOCUS       DAOSAB010000141         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_53982_length_1565_cov_3.113245, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000141 DAOSAB010000000
VERSION     DAOSAB010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53982_length_1565_cov_3.113245"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PK432_02700"
     CDS             <1..200
                     /locus_tag="PK432_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOK59868.1"
                     /translation="NSKEEAEKKDKKYFLKTFQTSSYSQSFNLPTTVNPDAVEAKFED
                     GVLTVRLPKAKETQAKQITIK"
     gene            296..508
                     /gene="rpmE"
                     /locus_tag="PK432_02705"
                     /pseudo
     CDS             296..508
                     /gene="rpmE"
                     /locus_tag="PK432_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006295093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            669..>1565
                     /locus_tag="PK432_02710"
     CDS             669..>1565
                     /locus_tag="PK432_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOK59869.1"
                     /translation="MDIESLKKKYTLQPVEEKKNELEKDIQDALLSHRDITQLSLDLS
                     YYTQLYTYYSNINKDINKYEQAEELLKETSDQELKNLATSEIDEVKKNIEESEEQIKK
                     LEIEKYFHDEDDSRNIILEIRAGAGGDEAALFAGDLFNMYRAYANKKGWEVQIIDSSV
                     SEDGGYKEVIAHITGKNVYKKLKYESGVHRVQRIPVTEASGRIHTSTATVAVLPEAHE
                     IDIKINPEDLRIEVMRASGAGGQCVNRTDSAVRITHIPTGIVVSCQETKHQAQNKEKA
                     MATLRSRLYEKKKREEDEKRSNM"
BASE COUNT          651 a          232 c          287 g          395 t
ORIGIN      
        1 gcaacagcaa agaggaagct gaaaagaaag ataaaaagta tttcttaaaa actttccaga
       61 ctagctcata ctctcaaagc tttaatctgc ctacaactgt aaatcctgat gctgttgagg
      121 cgaaatttga ggatggtgtc ttaactgtaa gattaccaaa agcaaaagag actcaagcaa
      181 aacagattac gattaaataa ataattactg gaagagacag attctactct gtctcttcct
      241 aaacatcttt agtttataga cacataatat atattctgat ataatcttct ctgttatgaa
      301 aaaagacatt catccaaaat ataaccataa tgtaaaagta gtatgctcat gcggaaacac
      361 ctttataaca ggctctactc tgcaaacaga tactatcaat gttgaactat gttcaaagtg
      421 tcaccccttc tatacaggtg aacaaaagat tgttgataca gaaaatgttg ttaagaaatt
      481 tgaagaaaga gctaaaaaga gctcacagat gtcatttaga agtaagagag aaaagatggc
      541 tgaaagaaaa gcaaaaagaa cagaaataaa gtcttctccc aatcttactc ttaaagacat
      601 gcttgcaaat gcaaacattt caaagtaaca gtaccccatt atatatttaa gtgatattat
      661 ccatatatat ggacatagaa agtttaaaga aaaaatatac attacaacct gtagaagaaa
      721 agaaaaatga gcttgagaaa gatattcagg atgctctact ctctcaccga gatattacac
      781 agctttcctt agacctttca tattatactc agctgtatac ttactactct aatattaaca
      841 aagacataaa caagtatgaa caggcagagg agttattaaa agaaacttct gatcaagaat
      901 taaaaaattt agcaacctct gaaatagatg aagttaaaaa aaatatagag gaaagtgaag
      961 aacagattaa aaaacttgaa attgaaaaat actttcatga tgaagatgat agtagaaaca
     1021 ttatcttaga aataagggct ggtgctgggg gagatgaggc agcactattt gctggtgacc
     1081 tttttaatat gtatagagca tatgcaaata aaaagggatg ggaggtacaa ataatagata
     1141 gtagtgtatc agaagatggt ggatacaaag aggtaatagc tcatattaca ggaaaaaatg
     1201 tatacaaaaa gcttaaatat gaaagtggtg tacacagagt tcaaagaata cctgtaacag
     1261 aagcctctgg gagaattcac acatcaactg caacagttgc agtacttcct gaagctcatg
     1321 aaatagatat caaaattaat ccagaagatt taagaattga agtaatgagg gcatcaggtg
     1381 caggaggaca gtgtgtaaac agaacagatt ctgcagtaag aataacacac attccaactg
     1441 gcattgttgt aagctgccag gaaacaaaac atcaagcaca aaacaaagaa aaggcaatgg
     1501 ctacattaag gtcaagattg tatgagaaaa agaaaagaga agaagatgaa aagaggtcta
     1561 atatg
//
LOCUS       DAOSAB010000142         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_54124_length_1562_cov_3.047777, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000142 DAOSAB010000000
VERSION     DAOSAB010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54124_length_1562_cov_3.047777"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PK432_02715"
     CDS             complement(<1..507)
                     /locus_tag="PK432_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOK59870.1"
                     /translation="MILSKRLIFIKFKNMFSLSPEFLYWIMVAVPGILIASTVHEYAH
                     GYTAYQLGDATAKAEGRLTLNPLKHIDPIGALSMLLFRFGWSKPVPINEYNFKKRELY
                     TALTALAGPVSNFILAALAGIINHFLVKDPQSLIAFLLYTFATINVSLAIFNLIPIPP
                     LDGHKIVRG"
     gene            535..750
                     /locus_tag="PK432_02720"
     CDS             535..750
                     /locus_tag="PK432_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HOK59871.1"
                     /translation="MAKICELCGKSTVAGKRIQHHHSIGWRFKAPKSKRAFKPNIRKV
                     KVDTDNGSEQIYVCMKCYKRLQKEAQK"
     gene            826..>1562
                     /locus_tag="PK432_02725"
     CDS             826..>1562
                     /locus_tag="PK432_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOK59872.1"
                     /translation="MSIIDSIWEILSYDLGMDLGTANTMVYLRGEGIVVSEPSVVAVD
                     KRSKQVLAVGQSAREMIGRTPANIVAVRPLKDGVISDFDTTQAMIHHFIKSAHSNYIK
                     GFKIPRPRVVVGVPSSVTEVERQAVIDAAKTAGAREVFIVEESMAAAIGIGLPIEEAS
                     GSMVVDIGGGTTGIAVLSLGDIVIDNTIRIAGDEMNQDIIDYVRDKYNILIGERSAED
                     IKIEIGSAAPLKEEKEVVIQGRDLLAG"
BASE COUNT          519 a          200 c          356 g          487 t
ORIGIN      
        1 acctcttact attttatgtc catcaagggg tggaattgga attaagttaa aaatggctaa
       61 agacacattt atagttgcaa atgtatatag tagaaatgct ataagtgatt gaggatcttt
      121 tactaaaaaa tgattaatta ttccagctag tgctgcaagt atgaagtttg aaactggacc
      181 tgcaagggct gtaagagctg tataaagctc tctcttctta aaattatatt catttattgg
      241 tacaggctta ctccatccga atctaaaaag aagcatagat agtgcaccga ttggatctat
      301 atgttttagt ggatttaatg tgagtcttcc ttctgctttt gcagtagcat ctccaagttg
      361 atatgcagtg tatccgtgtg catattcgtg tacagtagaa gctattaata ttcctggtac
      421 agcaaccatt atccagtaca aaaattcggg tgacaatgaa aacatattct taaacttgat
      481 aaatattaat ctttttgata atatcatctg gttaataatt gttcaaacaa atatatggca
      541 aaaatttgtg aattatgtgg aaaatctact gttgcaggaa aaagaataca gcatcatcac
      601 tcaatagggt ggagatttaa agcaccaaaa agtaaaaggg cttttaagcc taacattagg
      661 aaggtgaaag tggatacaga caatggttct gagcagattt atgtgtgtat gaaatgctat
      721 aaaagacttc agaaagaagc acagaagtaa agtgattata aaaattcctc gtatagggtg
      781 tgtattagtg gtaaaatcat ctgattaaga tgatatactt ttgatatgag tattattgat
      841 tcaatctggg agattctttc atatgattta ggtatggatt taggtactgc aaatacaatg
      901 gtatatttga gaggagaggg tattgtagta tctgagcctt ctgttgttgc agttgataaa
      961 aggagtaaac aggtacttgc agtagggcag agtgcaaggg agatgattgg aagaactcct
     1021 gcaaatatag tggcagtaag acctctaaaa gatggggtta tttcagattt tgatacaaca
     1081 caggcaatga tacatcactt tattaaaagt gctcattcta actatattaa aggttttaag
     1141 attccaagac ctagggttgt tgtaggtgtt ccttcctctg ttactgaggt ggaaagacaa
     1201 gctgtaattg atgctgcaaa aactgctggt gctagagagg tatttattgt tgaggagtca
     1261 atggcagcag cgatagggat aggtcttcca attgaagaag cgtctgggag tatggttgtt
     1321 gatattggtg gcggaactac aggtattgcg gttctttctt tgggagatat agttattgat
     1381 aatacaataa ggattgctgg agatgaaatg aatcaggata taattgacta cgtaagagat
     1441 aaatacaaca ttcttattgg tgaaagaagt gctgaggata taaagattga gataggatct
     1501 gcagcacctc ttaaggaaga gaaagaggta gttatacaag ggagagatct gcttgcaggt
     1561 tt
//
LOCUS       DAOSAB010000143         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_54231_length_1560_cov_3.088372, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000143 DAOSAB010000000
VERSION     DAOSAB010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54231_length_1560_cov_3.088372"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1560)
                     /locus_tag="PK432_02730"
     CDS             complement(<1..>1560)
                     /locus_tag="PK432_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59873.1"
                     /translation="FGVTSISNSDGTLTISPTTGNVIASLNLSNANIWTGAQTFSANT
                     YFPGSGIWNTSGNVGIGTTSPAYMLEVNNSNTTVAKMRKTVEGGDVLVLDAVNSATST
                     NWFKFTTNNETYTYGTFNTKPYAINIGAQFNIQLSPGSEGGTTQGGVTIGYNDYTTTP
                     VQKLGLMAGANSQVGLTVLGFSSSQTADLQRWGTYTSGDGVPNSFSYSTLAAISSSGG
                     AYFSGNVGIGTASPTQKLDVLGGIRLGTTSNVNNVLDTQAQGGAPSGSLYWGNAPLLT
                     SANLGSFGVTSISNSDGTLTISPTTGNVIASLNLSNANIWTGAQTFNANTYFPGSGIW
                     NTSGYVGIGTTSPTVALDVAGDIKASGVIRNVTVSGVTSTPASTAAKVVNIPNYTLTT
                     GDLLSITFTNGNSVSNPTLDVNSTGPKNIRVGNTNATTTTMTISAGATLILYYDGTYF
                     QMMGSQRTSDTDTNTYDRTYWGNAITAGTTIYSYKLLMQSTDGKWYPLTLENGTGTTK
                     TVSTQPFIMNSP"
BASE COUNT          443 a          321 c          251 g          545 t
ORIGIN      
        1 aggagaattc ataataaatg gctgtgtaga aactgttttt gttgtacctg tcccattctc
       61 tagtgtgagt ggataccatt ttccatctgt tgactgcata agcaatttat aactatatat
      121 tgtagtacca gcagtaattg catttcccca atatgttcta tcatacgtat tagtatcagt
      181 atcagaagtc ctttgagaac ccatcatttg aaaatatgta ccatcataat aaagaataag
      241 tgtagctcca gcagaaatag tcattgttgt tgtagtagca tttgtgttcc ctactcggat
      301 attctttgga ccagttgagt taacatcaag tgttgggttt gatacactat ttccatttgt
      361 aaatgtaatt gaaagaaggt caccagttgt taatgtataa ttgggtatgt ttactacttt
      421 ggctgcagtt gaagcaggag tacttgtaac tccagatact gtaacatttc taataacacc
      481 actggcttta atatcacctg caacatctaa agctactgta ggacttgtag ttcctatacc
      541 tacatatcct gatgtattcc atattccact ccctgggaaa tatgtgtttg cattaaatgt
      601 ttgtgctcct gtccatatat ttgcattact taaattcaat gatgctatta cattacctgt
      661 agttggagat attgttaatg tcccatctga attacttatt gatgttactc caaaactacc
      721 taaattagca gatgttaaaa gaggagcatt gccccaatac aaactacctg atggtgctcc
      781 accctgtgct tgtgtatcta acacattatt tacattactt gttgttccaa gtcttatacc
      841 acctaataca tctaatttct gtgtcggaga ggctgtccct attcctacat tacctgaaaa
      901 gtatgccccc ccactactac ttatcgcagc aagagttgag taactgaaac tattaggaac
      961 cccgtctcca cttgtataag taccccatcg ctgtaaatca gctgtctgac tgctactaaa
     1021 acctaacact gtaagtccca cttgagaatt agccccagcc atcaaaccca atttttgtac
     1081 tggtgtcgtt gtataatcat tataaccaat agtaactcca ccttgagtag ttccaccttc
     1141 tgaccctggt gaaagctgga tgttaaactg tgctccaata ttaatagcat aaggctttgt
     1201 attaaatgtc ccatatgtat atgtctcatt atttgttgta aatttaaacc agtttgtaga
     1261 cgttgctgaa tttactgcat caagtacaag tacatctcca ccttcgactg tctttctcat
     1321 ctttgctact gtagtattac tattatttac ttcaagcata tatgcagggc ttgtagttcc
     1381 tatacctaca tttcctgatg tattccatat tccactccct gggaaatatg tatttgcact
     1441 aaatgtttgt gctcctgtcc atatatttgc attacttaaa ttcaacgatg ctattacatt
     1501 acctgtagtc ggagatattg ttaatgtccc atctgaatta cttattgatg ttactccaaa
//
LOCUS       DAOSAB010000144         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_54423_length_1556_cov_3.019987, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000144 DAOSAB010000000
VERSION     DAOSAB010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54423_length_1556_cov_3.019987"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..>1556
                     /locus_tag="PK432_02735"
     CDS             12..>1556
                     /locus_tag="PK432_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59874.1"
                     /translation="MGSGLFVIIFLFFLFFLSRGFLRKRKDIVRAISSLSFGLFLLIL
                     LSMLSVLKVNIFSWIPYVKEFFVVGLPLTFSFQEIMLICGVSVFLNLFLLINSFKEEK
                     YQSIIFPIVALAISFISLPLFSVSQGILVPVLFFVFTILVCVLIWNKLGKDLKSLPII
                     VIIFAVLSFGFSIGFQYKSFTNSVLGKSYEAITPIKLGSDISWRVASSSIVENFSRGI
                     IGLGNDSFGVAYNLYKPAVDSTIMLGNTTFIVGANELFTTLANRGLLGVMAFILLVIV
                     SLKLLIKQISSDSTKGSSDILLLGLTHLFILFGSFFMPFSFLTFFMLFVSALLFVALY
                     NLEGNSEEFLLKFWAVNTGSVNKDIRKTMNGVNGFLTALFTIATVGIVVLLTLKVLNS
                     AYVVRAEAYGIEMGREHQEENVSIEVREEFLNRMAGYYDKALRYDSTDPYSNRRASLV
                     SIDVINLLSEKYKDAEETEKEALLSSITTWKNTAVDLSKRAIDTSPLTYSNWNTRASV
                     YTGLISV"
BASE COUNT          463 a          206 c          281 g          606 t
ORIGIN      
        1 atgatgggag aatgggtagt ggactttttg taataatatt tcttttcttc ttatttttcc
       61 tctcaagagg ttttctaaga aagagaaagg atatagttag agctatatcc tctttgtctt
      121 ttggtttatt ccttcttatt ctcctgagta tgctttctgt tttaaaagtt aatatattta
      181 gctggatacc ttatgtaaaa gaattttttg ttgttggatt acctcttaca ttttcattcc
      241 aagagattat gcttatatgt ggagtttctg tatttcttaa tctttttctt cttattaact
      301 catttaaaga agaaaagtat cagagcatta tattcccaat tgtagcccta gctatatctt
      361 ttatctccct tccactgttt tctgtaagtc agggtatttt agttcctgtt ctcttctttg
      421 tatttacgat tttagtttgt gtcttaatat ggaataaatt aggaaaagat cttaaatctc
      481 ttccaattat tgtaataata tttgcagttc tttcatttgg ctttagtata gggtttcaat
      541 ataaatcttt tacaaattct gtacttggaa aatcatatga ggctatcact ccaataaagc
      601 ttgggtctga tatatcatgg agagtagcaa gctcttccat tgttgaaaat ttctcaagag
      661 gaataattgg tttaggaaat gactcttttg gtgtggcata caatctgtat aaacctgctg
      721 ttgattctac gataatgctt ggtaatacaa cctttatagt tggtgctaat gaattattta
      781 ctacattggc aaatagaggt cttctaggtg ttatggcctt tattttgttg gttattgttt
      841 ctttgaaatt gttaataaaa caaatatcta gtgatagtac aaaaggtagc tcagatattc
      901 ttcttttagg attaactcat ctatttattc tttttggttc tttctttatg cctttctcat
      961 ttttgacatt ctttatgtta tttgtatcag cacttctttt tgtggcttta tataaccttg
     1021 aaggaaatag tgaggagttt ctgcttaagt tctgggcagt taatacaggc tcagttaata
     1081 aagatatccg taagacaatg aatggagtaa atggattttt gactgcattg tttactattg
     1141 ctacagtagg tattgtagta ttacttacct taaaggttct taatagtgca tatgttgtta
     1201 gagctgaagc atatggtata gaaatgggaa gagaacacca ggaagaaaat gtttcaatag
     1261 aggtaagaga agaattctta aatagaatgg caggatatta tgataaagca cttagatatg
     1321 attctactga tccgtattca aatagaagag caagtttagt ttctattgat gtaataaatc
     1381 tgttaagtga gaaatacaaa gatgcagagg aaactgaaaa agaggcttta ttatctagta
     1441 taacaacatg gaagaatact gcagtagatt tgtctaaaag ggcaatagat acttctccgc
     1501 ttacatattc taactggaat acaagagctt ctgtgtatac agggttaatt agtgtt
//
LOCUS       DAOSAB010000145         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_56974_length_1507_cov_2.828512, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000145 DAOSAB010000000
VERSION     DAOSAB010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56974_length_1507_cov_2.828512"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1507)
                     /locus_tag="PK432_02740"
     CDS             complement(<1..>1507)
                     /locus_tag="PK432_02740"
                     /inference="COORDINATES: protein motif:HMM:NF021607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HOK59875.1"
                     /translation="YNTSWLYRRSIFISNKETQAINEEVLIEYDTKSLIDAGKLQNDC
                     DDLRFVDSDDSTLLPYWIEGGCNSSQTQVWVRIPNLPESGKTIYMYYGNESAPNGQEE
                     WTGDFLLLKDSACTNEWKMESDTGQDFYQRFPKGASMYGETGGSASHSHPQTILTSSS
                     QISTSSSAKSGDSAKIADSHTHQITITSQDADALPPYLDMIICSNKNLVINQGLISLF
                     DFSIPSGFTRFSSLDKKFPRGNSTYGGSSGVTVHFHEILATATDGPSSETTAQEGSEI
                     YTASSTHTHTINQFITQTSENIPKYLSILYGQANNDSMAPIGVITMATALPPLGWTRF
                     SQLDEYYPYGSSSYGTTGGSSSHTHSISATTGSDTAMVGLSIGTPSISLPIKSTHTHN
                     IKGELQPYSNDPPYIKVLFIKKNSSKRATINNEEIPNQPPSSPTNLLTEGLVNPISVT
                     SQSPKFSAIYSDPNLDNGIYFEIEVNTKADFTGTSMWDSGKRSISPIISGQ"
BASE COUNT          430 a          266 c          282 g          529 t
ORIGIN      
        1 ctttgtccgc ttataattgg acttattgat ctctttccac tatcccacat acttgtacct
       61 gtaaaatcag cttttgtatt tacttcaatt tcaaaatata tcccattatc taaattagga
      121 tctgagtata ttgcactaaa tttaggagat tggcttgtta cactaattgg attaacaaga
      181 ccttctgtaa gtaaattagt aggagaagaa ggtggctggt taggaatttc ttcattatta
      241 attgttgctc ttttagaaga gttctttttg ataaagagta ctttgatgta tggaggatca
      301 tttgaatacg gctgtaattc tccttttatg ttatgagtat gtgttgattt aatagggaga
      361 gatatagaag gggtgccaat tgatagacca accattgctg tatcgctacc cgtagttgcg
      421 ctaatactgt gagtatgaga tgaacttcct cctgttgtac catatgatga gcttccatat
      481 ggataatact catcaagctg tgagaatctt gtccatccaa gcggcggaag ggctgtagcc
      541 atggttatta cacctatagg agccatactg tcattattag cttgtccata caaaatagat
      601 aggtatttag gaatattctc tgaagtttgt gtaataaatt gattaatggt atgagtgtgt
      661 gtacttgatg ctgtgtatat ttcactacct tcctgagcgg tcgtttcaga acttggtcca
      721 tctgttgcgg ttgctaagat ttcatggaaa tgcactgtta caccgctgct tcctccatat
      781 gtagagtttc ctcttgggaa tttcttatca agtgaagaga atcttgtaaa tccagaagga
      841 attgagaaat cgaataaaga gatgagacct tgattaataa ctaagttttt gttagagcat
      901 attatcatat caagatatgg aggtaatgca tctgcatctt gtgaagttat tgttatctga
      961 tgtgtatgag aatctgcaat tttagcacta tcaccgcttt ttgcagaact actagtacta
     1021 atttgagaac tgcttgttaa tatcgtctga ggatgagaat gagaagcact cccgcctgtc
     1081 tctccataca ttgaagcacc tttagggaat ctttgataaa aatcttgtcc tgtatcagat
     1141 tccattttcc attcatttgt acatgcagag tcttttagca aaagaaaatc tcctgtccac
     1201 tcctcttgcc cattaggagc agactcattt ccatagtaca tatatattgt ttttccactc
     1261 tctggcagat taggtatcct tacccaaacc tgtgtttgag aagagttgca cccgccttct
     1321 atccaatatg ggagcaatgt tgaatcatca ctatctacaa atctaagatc atcacaatca
     1381 ttttgaagct ttccagcatc tattaaagat ttagtgtcgt attcaattaa aacttcttca
     1441 tttatagctt gagtctcttt atttgaaata aagatagatc ttctgtaaag ccaacttgtg
     1501 ttatacc
//
LOCUS       DAOSAB010000146         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_57336_length_1501_cov_2.800830, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000146 DAOSAB010000000
VERSION     DAOSAB010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57336_length_1501_cov_2.800830"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PK432_02745"
     CDS             <1..961
                     /locus_tag="PK432_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOK59876.1"
                     /translation="YSGGKKLYDWLNENKGKTVKDFEKEFNIKVKGDGSIYIGEEPPK
                     TDDEWEAESDVFDTWFSSGQWPFSTLGGPQGEDFKKYYPTQTMIHARDILFWWSARML
                     MLGIYRTGQVPFNTIFLTGMILASDGSKMSKSKGNGVEPQEVFDKYGADALRLWYYSD
                     ALPGSNAPLREEKIKGNRNFVNKIWNASRFIILNIEESEIERVANFKVDMNLERVKMT
                     EKHIQRVSKYIEKYQFNLGAEAIREFFWHQLCDVWIEEVKKEIKDEDIGSAKRIEKLS
                     ELLYILKRNLMIMHPFVPFVTESVWQELVRLHLAEGLLMSQQI"
     gene            complement(1015..>1501)
                     /locus_tag="PK432_02750"
     CDS             complement(1015..>1501)
                     /locus_tag="PK432_02750"
                     /inference="COORDINATES: protein motif:HMM:NF039287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOK59877.1"
                     /translation="GNFINRTLTFVNTKFNNTVPQGKTDGVVLKNINNAFKNVGEYIE
                     KCEFVKASEELLRLGDFANKYFNDEAPWLSYKNNLKDCENTIFNSIQLVNALRILLKP
                     FTPNSSEKLKIMLNIQDEYDQNIDLEKNGKVSKIQNNWKFTQIDSCTHLNTPEILFEK
                     I"
BASE COUNT          502 a          193 c          300 g          506 t
ORIGIN      
        1 gtacagtgga ggcaaaaagc tttatgattg gttaaatgaa aacaaaggaa agacagttaa
       61 ggattttgaa aaagagttta atattaaggt aaaaggtgat ggaagcatat atataggaga
      121 ggagccacct aaaacagatg atgagtggga ggctgaaagt gatgtatttg atacatggtt
      181 tagttctgga cagtggcctt tttctacatt aggtggacct cagggagagg attttaagaa
      241 atactatcct acacaaacaa tgattcatgc aagagatatt ctgttttggt ggtctgctag
      301 aatgcttatg cttggtatat acagaacagg gcaggtacct ttcaatacaa tctttttaac
      361 agggatgatt ttagcctctg atggctcaaa gatgtctaag agcaagggaa atggagttga
      421 gcctcaagaa gtatttgata aatacggtgc tgatgctctt aggctttggt attattcaga
      481 tgctcttcct ggttcaaatg ctcctttaag agaagaaaag ataaagggaa atagaaattt
      541 tgttaacaag atatggaatg caagtagatt tatcatctta aatattgagg aaagtgagat
      601 agagagagtt gctaatttta aagtagatat gaatcttgag agagtaaaga tgacagagaa
      661 acacatacaa agggtaagta agtatattga aaagtatcag tttaatcttg gtgccgaagc
      721 aataagggag tttttctggc atcagctttg tgatgtgtgg atagaagagg ttaaaaaaga
      781 gattaaagat gaggatattg gttctgcgaa gagaattgag aagctttcag agcttttata
      841 cattttaaaa agaaacttaa tgattatgca tccttttgtt ccttttgtta cagaatctgt
      901 atggcaggag ttagtaaggt tacaccttgc agaagggctt ttaatgagtc agcagatata
      961 aatacttcaa ttgaaagacg atggatcaac tcatttttga gaataaagtc tttattatat
     1021 cttctcaaac aaaatctctg gagtatttaa atgagtacaa ctatctattt gagtaaactt
     1081 ccagttattt tgtattttac ttaccttgcc attcttttct aaatcaatat tttgatcata
     1141 ttcatcttgt atattaagca taattttaag cttttcagag ctgttgggtg taaaaggctt
     1201 taagagtatt cttaatgcat taactaactg tattgagttg aagatagtat tttcacaatc
     1261 tttaagatta ttcttatatg ataaccatgg ggcttcatca ttgaaatact tgtttgcaaa
     1321 gtctcctaga cgaagaagtt cttcacttgc ttttacaaat tcacacttct ctatatactc
     1381 tcctacattc ttaaaagcat tatttatatt ttttagtact acaccatctg tttttccttg
     1441 aggaactgta ttattaaact ttgtattaac aaatgtaagt gttctgttta taaaattacc
     1501 a
//
LOCUS       DAOSAB010000147         1498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_57488_length_1498_cov_3.302841, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000147 DAOSAB010000000
VERSION     DAOSAB010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57488_length_1498_cov_3.302841"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1498
                     /locus_tag="PK432_02755"
     CDS             <1..>1498
                     /locus_tag="PK432_02755"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOK59878.1"
                     /translation="ENGYSPSNYIQGKSTLNSTLPDPVTDLSIADTSIKDAKLWRASI
                     TWSEPVYKGDGKLTYIVQRSFNGIDWTTVGSTTGITYSDIVPSSRLYYYRVGVMDGTD
                     QSKDNPTFSISISAMIEGRYTQPAQLVSEVEIANLGTRKATIVWVTERNSDTKISYGL
                     TSGEYFEEEAYNSKQVTEHEITLNNLQPETTYYFKAKWTDSDGNTGESQEISFRTADS
                     PQVYSSKVDMVGLNYAVISFEVFGATSASVIYGRSFAYTAQKEINTSPTRSKYSIVLD
                     GLDDGVQYSYKIRLMDAEGYIYDSIENNTFTTPPKPKISNVRVQEIREVASPTVVFSW
                     DTNTHTNSIVRYREDIQDSREIDKVDMKMVKGEHKMEISGLVPTTNYIAYVEGVDDYG
                     NRAVSEVVRFTTQTDTRPTKIFNVKVEQDLLGRSIQTDRSRSAQLIVSWETDEPATSK
                     VNFGEGGAGVYTSSSRMDQELRTNHLVIVSGLTPSKVYSLEIESGDDSG"
BASE COUNT          534 a          207 c          331 g          426 t
ORIGIN      
        1 tgagaatggg tactctccca gtaactatat tcaagggaag tctacattaa atagcacttt
       61 acctgaccca gttactgatt taagtatagc agatacatca attaaagatg ctaagctgtg
      121 gagagcaagt attacatgga gtgagcctgt atataaagga gatgggaagc ttacatatat
      181 tgtccagaga agttttaatg gaattgattg gacaactgta ggaagtacaa ctggtattac
      241 atattcagat attgttcctt caagcagact ttattattac cgagttggtg taatggatgg
      301 aactgaccag agtaaagata atcctacttt ttcaatatct atttctgcaa tgatagaggg
      361 aagatacaca cagcctgcac agttagtttc tgaggtagaa attgcaaact taggaacaag
      421 gaaagcaaca atagtatggg taactgaaag aaacagtgat acaaaaattt catatggttt
      481 aactagtggg gagtattttg aggaggaagc atacaactct aagcaggtaa ctgagcatga
      541 gattacatta aataatcttc aaccagaaac tacatattac tttaaagcaa agtggactga
      601 ttctgatgga aatactggag aaagtcaaga gatatcattt aggacagcag attctcctca
      661 agtatactcc agcaaggtag atatggttgg tttaaattat gcagttattt cttttgaggt
      721 atttggtgcg acaagtgcat ctgtaatata tgggaggtcc tttgcatata ctgcacaaaa
      781 agagataaac acatcaccta caagaagcaa atactctatt gttttagatg gtttagatga
      841 tggggtacag tatagttata agataagact gatggatgct gaagggtaca tatacgattc
      901 tattgaaaac aatacattta ccacacctcc taaacctaaa atatcaaatg taagagtgca
      961 ggagataaga gaggttgcaa gtcctactgt agtatttagc tgggatacaa atacacatac
     1021 taattctatt gtaagataca gagaagatat acaagattca agagagattg ataaagttga
     1081 tatgaagatg gtaaagggag aacataaaat ggaaatatct ggtttagtac caacaactaa
     1141 ctatattgcc tatgtagaag gagtagatga ttatggaaat agggctgtct cagaggttgt
     1201 gagatttact acacaaactg atactagacc tacaaagata tttaatgtta aagttgaaca
     1261 ggatcttttg ggtaggtcaa ttcaaacaga tagaagcagg agtgctcagt tgatagtaag
     1321 ctgggagact gatgaacctg ctacaagtaa ggtaaacttt ggtgagggtg gtgcgggtgt
     1381 atatacatct agttctagaa tggatcagga gctgagaaca aatcatttag ttatagtttc
     1441 aggcttaact ccatctaaag tgtatagttt agaaattgaa tctggagatg attcaggc
//
LOCUS       DAOSAB010000148         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_57976_length_1489_cov_4.524407, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000148 DAOSAB010000000
VERSION     DAOSAB010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57976_length_1489_cov_4.524407"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PK432_02760"
     CDS             <1..1054
                     /locus_tag="PK432_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59879.1"
                     /translation="AIQTTITKKVAEKKDFDLKDFKKKSTIQLTLIAAVISIIFFAFG
                     KQISNIANIPVQYIPALTLVVFTAIINPIAKGFLLGLEKITQFNTVMIAETLFKFAIG
                     YFALQTSSQITTITLAFAVPFLISTLIILPFTNTKSKKKPEQELKLNYKQIFLIFITF
                     FMLNIPFTLDLILVNPAVRASYGALSLIGKIVYFGAVTIANLMISRLANTQGQSRKKN
                     LLISLGISAVIGIVICIIYWLFTEQIVNIVFGGLYKEITRYVVPYSIAMIGYAISYMV
                     ITSELVNDSYIHIYLLLFTSLLQGVLYKINNNSLYDAFLNQIIVYGVLFFFVLILLIF
                     KTLKTNGKERTKEEQI"
     gene            1020..>1489
                     /gene="mscL"
                     /locus_tag="PK432_02765"
     CDS             1020..>1489
                     /gene="mscL"
                     /locus_tag="PK432_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOK59880.1"
                     /translation="MEKKEQKKNKFKKGWEDFKSFALKGNVISMAVGVVIGAAFKDLI
                     NSLVNNVFTPPIGFLTAKLDFSKLFITLGKTQYETLEEAQAANAVVIQYGLVLNTLLS
                     FLITALVLYLVSKAVSKATQKEKDAKPVKKTKKCPYCMSEINIKAKRCPFCTSE"
BASE COUNT          567 a          239 c          225 g          458 t
ORIGIN      
        1 agcaatacaa acaacaataa caaagaaagt agcagagaag aaagattttg accttaaaga
       61 tttcaaaaag aagagtacaa tacaactaac cttaatagct gcagtaatct ctataatatt
      121 ctttgcattt ggaaaacaga tatccaatat tgcaaacatt ccagtacaat atatacctgc
      181 actaacacta gtagtattta ctgcaattat aaacccaatt gcaaagggat tcttacttgg
      241 tcttgagaaa attacacaat ttaatacagt aatgattgca gagacactct ttaaattcgc
      301 aatagggtat tttgcacttc aaacaagctc acagattaca acaataacat tagcatttgc
      361 agttcccttc ctaatatcaa cattaattat tcttccattt acgaatacaa aaagcaaaaa
      421 gaaacctgag caggagctta agcttaatta taaacaaata tttctcatct ttatcacctt
      481 ctttatgctt aacatcccct tcactcttga ccttatactt gtaaacccag cagtaagagc
      541 atcatatgga gcactttctc taataggaaa gattgtatac tttggagcag ttacaatagc
      601 caacttaatg atatcaagac ttgcaaatac acaaggacag tcaagaaaga agaatctctt
      661 gatatcattg ggaatatctg cagtaattgg gattgtaata tgtataatat attggctctt
      721 tacagagcag attgtaaata ttgtatttgg tgggctgtat aaagaaatca ctagatatgt
      781 tgttccttac tcaattgcta tgatagggta tgctatatca tacatggtaa taacctcaga
      841 acttgtaaat gattcataca tacacatata tctactactg ttcacatctt tacttcaagg
      901 tgtactgtat aaaataaata ataatagttt gtatgatgca ttcttaaatc aaataattgt
      961 atatggtgta ttattcttct ttgttttaat acttctaatt tttaaaactc ttaaaacaaa
     1021 tggaaaagaa agaacaaaag aagaacaaat ttaaaaaagg ctgggaagat tttaagtctt
     1081 ttgcactaaa aggcaatgtc atatcaatgg cagttggtgt tgtaattgga gcggcattta
     1141 aggatttaat aaactcatta gtaaataatg tgtttacacc accaataggg tttcttactg
     1201 cgaaattgga tttctctaag ctatttataa ccttaggaaa aacacaatat gaaacacttg
     1261 aagaagcaca agctgctaat gcagtagtta tacagtatgg attggtatta aacacattac
     1321 tttcattctt aatcacagct ttagtactat accttgtaag caaggctgta agcaaagcaa
     1381 cacaaaagga aaaagatgca aagccagtaa agaaaacaaa gaaatgccct tactgtatgt
     1441 cagaaataaa tattaaagca aaaagatgtc cgttttgtac aagtgaatt
//
LOCUS       DAOSAB010000149         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_58309_length_1484_cov_2.088873, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000149 DAOSAB010000000
VERSION     DAOSAB010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58309_length_1484_cov_2.088873"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..874)
                     /locus_tag="PK432_02770"
     CDS             complement(131..874)
                     /locus_tag="PK432_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOK59881.1"
                     /translation="MNLEDIILDFFDFVGNIDFNMFGTLFVVALVVFWLVIIGWVWVD
                     SSERTSKKGLKVGYILLVIFFNIFGLIIYLIIRPSETIEEIYWEDLERRYLKYETSEL
                     GDCPRCGSQLYPGYVFCTNCGYRLKVKCPQCGVLIDKDHVFCEYCGFKIKERATVQEE
                     HPTVKVMEEQITATKREAEKTVESKMTKYKTGNSFVVKLGNSIVSSVSKLGGKKKGKD
                     EEQVVQESKNQQKNVQSSKQKKKKKKKKR"
     gene            complement(903..986)
                     /locus_tag="PK432_02775"
     tRNA            complement(903..986)
                     /locus_tag="PK432_02775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(950..952),aa:Leu,seq:caa)
     gene            complement(1029..>1484)
                     /locus_tag="PK432_02780"
     CDS             complement(1029..>1484)
                     /locus_tag="PK432_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOK59882.1"
                     /translation="PGQNINQIRTKARRLMLEQGVDIIFVDYLQLMHGNTKESRALEV
                     GEVSQGLKNPAKELNVPVVALSQLSRAIELMQNRRPQLSDLRESGSIEQDADVVIFLD
                     REETWNPDTEKKGSAEVFVAKHRNGPTGIVELAFIKEIASFRNLYKEKN"
BASE COUNT          437 a          346 c          167 g          534 t
ORIGIN      
        1 caccctctcc aaaatatata aagaccaaga aaaaacaata tgacatacat cacaaatgga
       61 tttattccct gtatatactc ttttatttga gaaaaaattt ctcccacact tcttttatta
      121 tactttattc ttatctcttc tttttcttct ttttcttctt ttgtttacta ctctgtacat
      181 tcttttgctg attcttactt tcctgtacta cctgctcctc atcttttccc tttttctttc
      241 ctcccagctt tgatacagaa gaaactatgg aatttcctaa tttaacaaca aaactatttc
      301 ctgtcttgta ttttgtcatc tttgattcaa cagtcttctc tgcttctctc tttgtcgcag
      361 taatctgctc ctccattact ttaacagtgg gatgttcctc ttgcacagtt gctctctcct
      421 taatcttaaa accacaatac tcacaaaata catgatcctt atctattaac acaccacatt
      481 gaggacattt aaccttaagt ctgtatccac aatttgtaca aaagacatac cctggataaa
      541 gctgagagcc acatctaggg caatccccaa gttcagatgt ctcatatttt aagtatcttc
      601 tctctaaatc ctcccagtat atttcctcta ttgtctcact aggtctaata attaaatata
      661 ttattaatcc aaaaatatta aagaatatta caagcagaat atatcctacc tttaatcctt
      721 tcttagaagt tctctcactt gaatcaaccc atacccaccc tattattacc aaccagaata
      781 caactaaagc taccacaaag agtgttccaa acatattaaa atcaatattt cctacaaaat
      841 caaaaaaatc tagaataata tcttctaagt tcatattttt aaatattatt ttattttaaa
      901 aatggtcgag gagggagttg aaccctcaca ctattgctag catgggattt tgagtcccac
      961 gtgtctgcca attccaccac tcgaccacac cctgtaatta tatctcatta aaacctttgt
     1021 tgtatacact aattcttctc tttgtacaaa ttcctgaaac tggcaatttc tttgataaag
     1081 gctagttcaa ctatacctgt aggaccattt ctatgttttg caacaaatac ttctgcactc
     1141 cccttctttt ctgtatctgg attccatgtc tcttccctat ctaaaaatat aacaacatca
     1201 gcatcttgtt ctattgatcc agattctctc aaatctgata actgaggtct tctattctgc
     1261 attaactcaa ttgcacgaga cagctgtgat agtgcgacaa cagggacatt aagctccttt
     1321 gctggatttt taagaccctg agacacttca cctacttcaa gtgctctgct ttcctttgta
     1381 ttaccatgca taagctggag atagtcaaca aatataatgt caacgccctg ttctagcata
     1441 agtcttcttg cttttgttct aatctgatta atattttgac cagg
//
LOCUS       DAOSAB010000150         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_59081_length_1471_cov_2.872881, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000150 DAOSAB010000000
VERSION     DAOSAB010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59081_length_1471_cov_2.872881"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PK432_02785"
     CDS             <1..364
                     /locus_tag="PK432_02785"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOK59883.1"
                     /translation="MSAVIENIPKDTIRYAMGVGKPEDILNAAKIGYNIFDTVLVTRN
                     ARHGTVYTSQGVVRIKNSEMALDNSPVDANCGCECCRNYSRAYLHHMLKSNEATALTL
                     LTIHNLTFYQDIIKKLNQ"
     gene            598..>1471
                     /locus_tag="PK432_02790"
     CDS             598..>1471
                     /locus_tag="PK432_02790"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HOK59884.1"
                     /translation="MEAIIMVIAALVGGASVYSYLRFGKKWVNVKDIPEEEILKVADK
                     VVSKRIEEAEKEVEENKRKAEEHLRQLRKEAQEHEELLLEREKKLNERTRMLDKKSEE
                     LEMASESLKKMQRGVEKTRLQLKEELQKISGLTQEEARQRLMSEIDEDIKDYEAKKIR
                     QAQKKVEEVADEKAKEILVESMQRIVTDYVGETTTSTIKVDDDKVKGRIIGKEGRNIR
                     AFEKLTGVDVIIDESPNTIALSSFDPLRREIAAVALNKLIGDGRIHPSSIEEAIRKAK
                     NEISIEIKKNGEILA"
BASE COUNT          578 a          169 c          313 g          411 t
ORIGIN      
        1 gatgagtgct gtaattgaga atatccctaa ggatactata aggtatgcaa tgggggtagg
       61 aaagcctgaa gatatactta atgcagcaaa aatcgggtac aatatttttg atacagtact
      121 tgttactcgc aatgcaagac atggtactgt atatacttct cagggggtag taaggataaa
      181 gaatagtgag atggctttgg ataactctcc tgttgatgct aactgcggct gtgaatgctg
      241 tagaaactat tctcgtgcat atcttcatca tatgctcaag agcaatgaag ctactgctct
      301 tacacttctt acaattcata atcttacctt ttatcaagat attattaaga aattaaatca
      361 ataatctctg gcaagaatct cttaaagatg gtattatata tttgtcctta attgatatcg
      421 ttttttaatt gaaggacagg aaatagaaat actaagatat tttttgaatt aatgaaaatt
      481 tttaagaaaa tatataaata caaaaaaaat aaaacagaag atactaatta tatacggagt
      541 cttttgtaac tcttagttgt ttcttttcct aaatgtataa attttaaata tactcatatg
      601 gaggcaataa ttatggttat tgcagcctta gtagggggtg catctgtata cagttacctt
      661 agatttggaa agaaatgggt aaatgtaaaa gatattcctg aggaagaaat acttaaggtt
      721 gcagataaag ttgtatcaaa aagaatagaa gaagcagaaa aggaggtaga agagaataaa
      781 agaaaggctg aagagcattt aagacagctt agaaaagaag cacaggagca cgaagagctg
      841 ttattagaaa gagaaaagaa attaaatgag agaactagaa tgcttgataa aaaaagtgaa
      901 gagttggaaa tggcatctga atcattaaag aagatgcaga gaggtgttga aaagacaaga
      961 cttcagctta aggaagaatt acaaaaaatc tcaggtttaa cacaagaaga agcaagacag
     1021 agattaatga gtgagattga tgaggatatt aaggattatg aagctaaaaa gattagacag
     1081 gcacaaaaga aagttgagga ggtggcagat gaaaaagcaa aagagatttt agttgaatca
     1141 atgcaaagga ttgttacaga ctatgttggt gaaactacaa cctctactat taaagttgat
     1201 gatgataagg taaaaggaag aataattgga aaggagggaa gaaatattcg agcatttgaa
     1261 aagcttacag gtgttgatgt aatcattgat gaatctccta atacaattgc actctcctct
     1321 tttgatccat taagaaggga gattgctgct gttgcactta ataaactgat aggagatgga
     1381 agaattcacc caagttcaat tgaggaggct attagaaaag caaagaatga aatttcaatt
     1441 gaaattaaaa agaatggtga aattcttgca g
//
LOCUS       DAOSAB010000151         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_59431_length_1465_cov_3.517730, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000151 DAOSAB010000000
VERSION     DAOSAB010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59431_length_1465_cov_3.517730"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="PK432_02795"
     CDS             complement(<1..1442)
                     /locus_tag="PK432_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59885.1"
                     /translation="MGDGLYSLSPEGMSSKNIDPKFKAIVADKDLKNYYDTLTTYYFG
                     LQKMMVGQKMGYKIPGFAASKIENFKRLGFKKGLSKEMAIAIDKHLKFQSSEQDFSEN
                     IFGDLGGIIRGRFTRQLGEELQTTDSINAIYKFAVEAHYNKAMQEAQPQVEGVIEYYK
                     EQIKIMKNSKSTDPAIQAQINKRIKEWTSAVDSLEYEKRKFIQGQIDDAQDRKIKKGL
                     NLLFQYISFMRLGFDITNQVKNYTAGSVQFWIAAGNGSKHYTDKTAIQAYRQVYGVNG
                     FLHNYFADWGKISNLHESTMLYRIFNPLQKDMIKYVDEITGGRTRRLGNKLTNFMELA
                     YLVQDKGDTLIGLVTMYAVLFHYKFNVIDPITKQPVLDANGNNVQVSAAECYIKGEDG
                     TLEIRNDVDYTKEDQLRLKRIIIAEMRRAQGNYAKSDQTRIEAKTQGKLLFFYRKYFI
                     PLFLNRFGTLRPSWETGEVSLGYWRALVQA"
BASE COUNT          457 a          262 c          155 g          591 t
ORIGIN      
        1 aatgcttgta ctaatgctct ccaatatcct aaagaaactt ctcctgtttc ccatgaaggt
       61 cttaaagtac caaatctatt taagaataaa ggaataaaat actttctata aaagaataat
      121 aatttacctt gtgtcttagc ttctattcta gtttgatctg attttgcata gttaccttgt
      181 gctctacgca tttctgctat aataattctt tttaatctta actgatcttc tttagtataa
      241 tcaacatcat ttcttatttc taatgtccca tcttctcctt ttatataaca ttctgcagca
      301 gaaacttgta cattatttcc attagcatcc aatactggtt gttttgtaat aggatctata
      361 acattaaatt tataatgaaa taatacagca tacattgtaa caagtccaat taaagtatca
      421 cctttatctt gcacaagata agctaattcc atgaaattgg ttaatttatt acctaatctt
      481 ctagttctac cacctgttat ttcatctaca tatttaatca tatctttttg taaaggatta
      541 aatattctat ataacattgt agattcatga agattactta tcttacccca atctgcaaaa
      601 taattatgaa gaaatccgtt aacaccatat acttgtctat aagcttgtat agcagtttta
      661 tcagtataat gtttacttcc attaccagca gcaatccaaa attgtactga acctgctgta
      721 taattcttta cttgattagt aatatcaaaa ccgagtctca tgaaactaat atactggaac
      781 agtaaattaa gtcctttttt tatttttctg tcctgagcat catcaatctg tccctgtata
      841 aactttcttt tctcatattc taaactatcc acagcactag tccattcttt aattctttta
      901 ttaatttgag cttgtatggc aggatctgta gatttagaat ttttcattat ttttatttgt
      961 tctttataat attcaattac accttctact tgtggttgtg cttcttgcat tgctttatta
     1021 taatgagctt ctacagcaaa tttatatata gcattaatag aatctgtagt ttgtaattct
     1081 tctcccaatt gtcttgtgaa tctacctcta ataattcctc ctaaatcacc aaatatgttt
     1141 tcagaaaaat cttgctcact actttgaaat ttaagatgtt tatctatagc aatagccatt
     1201 tctttactta gacctttttt aaatcctaat cttttaaaat tttctatttt acttgctgca
     1261 aatcctggta ttttatatcc cattttttgc cctaccatca ttttttgtaa accaaaataa
     1321 taagtagtga gagtatcata ataatttttt aaatctttat cggcaacaat agctttaaat
     1381 ttaggatcta tatttttact tgacatacct tctggagata aactatatag accatctccc
     1441 atagatataa ctccttttgg taaag
//
LOCUS       DAOSAB010000152         1462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_59613_length_1462_cov_3.135750, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000152 DAOSAB010000000
VERSION     DAOSAB010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59613_length_1462_cov_3.135750"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..>1462)
                     /locus_tag="PK432_02800"
     CDS             complement(153..>1462)
                     /locus_tag="PK432_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOK59886.1"
                     /translation="TSFSQKPAVQVGQKFKKGDILVDGPTCVNGELAIGTNLRAALMF
                     YEGYNYEDAVVISERIVRDDLLTSVHIREHEIEVKDTELGPEVISADIPHVNDRILQK
                     LDESGIVREGVKVKAGDILVGVVAPRGEKELTAEERLLRAIFGESASDVRDNSLRLPH
                     GEEGIVIRTQRLSVQNGDKLGPGVLEQVKVWVADTKKLGYGDKISGRHGDKNTVSAIR
                     PIEDMPFTEDGQPVDIVLTPTFLKRMNMGQAEEVHFGQYAQLLNEKFAFPLFEDVNME
                     WLEKEMRKKGFEMEEKVDLYDGRTGKKFPRKVTVGMKYILKLHHIADEKVHARSTGPY
                     TAVTQQPLSGKAQRGGQRFGEMEVWALEAHGAPYALQEMLTIKSDDIKGRSSAYKAII
                     HGEKIENVNVPESFKVLIKELNALCLNMELISKKSQEVIENEQ"
BASE COUNT          414 a          338 c          192 g          518 t
ORIGIN      
        1 caggttctgc tctgaatgtt ctatagttta tagtttctgc ttttgtaact tccccatatg
       61 accagtcaag tatctgctct ggcgatgcaa gagtaagtct taatgcatca aaatcttcaa
      121 aagcattttt atgattacga ttatggtttt gattattgtt cattctcaat aacctcctgt
      181 gattttttag aaattaattc catatttaag caaagtgcat ttaattcttt aattaatact
      241 ttaaaggatt caggaacatt aacattctct atcttttcac catgtattat tgctttatat
      301 gcacttgacc tacctttaat atcatctgat ttaatagtaa gcatctcttg cagtgcatat
      361 ggtgcaccat gtgcttccaa agcccaaact tccatctctc caaatctttg accacctctt
      421 tgtgctttac cactaagagg ctgctgagta acagcagtat atggtcctgt agatctagca
      481 tggacttttt catctgcaat atgatgaagt ttaagtatat acttcatacc aactgtcacc
      541 ttccttggga atttcttacc tgtccttcca tcatacaaat caactttctc ttccatttca
      601 aatccttttt tcctcatttc tttttcaagc cattccatat ttacatcctc aaaaagagga
      661 aatgcaaatt tctcattaag aagttgtgca tactgaccaa aatgaacctc ttctgcctgt
      721 cccatattca ttcttttaag aaaagtagga gtaagaacta tatcaacagg ctggccatct
      781 tctgtaaacg gcatatcttc aattggacga attgcagaga cagtattttt atccccatgt
      841 cttccagata ttttatctcc atatcctaac tttttagtat cagcaaccca taccttcacc
      901 tgctctaata caccaggacc taatttatca ccgttttgta cagacaatct ctgagtacgg
      961 ataacaatgc cttcttctcc atgtggaagt cttaatgagt tatctcttac atcacttgca
     1021 gactctccaa atattgctct aagcaatctt tcttctgcag ttaactcctt ctctcctctt
     1081 ggtgcaacaa caccaacaag aatgtctcct gccttaactt taaccccttc tcttacaatt
     1141 cctgattcat caagcttttg aagtattcta tcatttacat gaggaatatc agctgaaata
     1201 acctcaggac caagctctgt atcttttact tcaatttcat gctcccttat atgaactgat
     1261 gttagtaaat catctcttac aattctttca gaaattacta cagcatcttc atagttataa
     1321 ccctcataaa acattaatgc tgctctcaga ttcgtaccta tagcaagttc accattaaca
     1381 catgtagggc catctacaag gatgtctcct ttcttaaact tctgtcctac ctgcacagca
     1441 ggtttctgag agaatgaagt at
//
LOCUS       DAOSAB010000153         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_59734_length_1460_cov_2.524555, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000153 DAOSAB010000000
VERSION     DAOSAB010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59734_length_1460_cov_2.524555"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1460)
                     /locus_tag="PK432_02805"
     CDS             complement(<1..>1460)
                     /locus_tag="PK432_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59887.1"
                     /translation="DYYDKFEADVQEILEKQEQEKYKDTPIEELEKVPQGADFVKIDP
                     VDLINKIRNTEILNTLAQASISTGIDFKMMSAEEAKTELAGKYVGYNGQPLVLLLNKL
                     IYIPENLSSTTELADLMIPFITSLRRSKKNIFNIQYNKIINSSIGPMLVERAKQNNPD
                     ASYDNEYFQELVIQEALREDANLKKLAKLSQAGAETIDPLFKEAITAIVAHARESIRS
                     KIQGIKQTSISTNTTLEEFGTLVENAGKKFTTEGITSSDVAKYNQEIQKGVDEITTAV
                     NNDESLAILNNIIQNFIKTNSKQLSEIKDNIYFDIRQSLSDESGRFLRDIAERLKYFR
                     KENADVYDYLDNSFRAKALVSSLLTLNKTLDKILEFLNGLERQKLSVEELAEKVAHYN
                     YLLQSWGNFVETTRRNLKEMGVAEESTIYGFLARLDSKIEDGAAIAKKLNNNVATEHI
                     AVLLGDFSTKQRKYYDDQIADLKKQAYSAKRDRQIK"
BASE COUNT          426 a          258 c          196 g          580 t
ORIGIN      
        1 cttttatctg tctatctctt ttagctgaat aagcttgttt ttttaaatca gctatttggt
       61 catcataata ctttctttgt ttagtactaa agtctcctaa taacacagca atatgttcag
      121 tagctacatt attattaagc ttttttgcta tagctgcacc atcttctatt ttagaatcaa
      181 gtctagcaag aaatccatag attgtacttt cttctgcaac acccatctct ttaagatttc
      241 tgcgggttgt ttctacaaaa ttaccccagc tttgcagaag atagttgtag tgagcaacct
      301 tttcagcaag ctcttctaca gataattttt gtctttcaag gccatttaag aactcaagga
      361 tcttatctaa ggttttatta agagttaata aagagctaac taaggccttg gctctaaaac
      421 tattatctag atagtcatat acatcagcat tttcttttct aaaatactta agcctttcag
      481 caatatctct taagaatctt ccagactcat cggataaact ttgtctaata tcaaagtaga
      541 tattatcttt tatctcactc aactgtttac tattagtctt aataaagttt tgtataatat
      601 tattaagaat agctaatgat tcatcattat ttacagcagt tgtgatttca tcaacacctt
      661 tttgaatctc ttgattatat tttgctacat ctgagctggt tataccttct gtagtaaatt
      721 tctttcctgc attctcaaca agggttccaa actcttctaa agtagtatta gtacttatag
      781 aagtttgttt tattccttgt atctttgatc ttatactttc tctagcatgt gcaacaatag
      841 ctgttatagc ttccttaaat aaaggatcta tcgtttcagc tcctgcttga gataacttag
      901 ctaacttttt aagattagca tcttctctca aagcttcttg tataacaagt tcttgaaagt
      961 attcattatc ataagaagca tccgggttat tttgcttggc tctttcaaca agcattggac
     1021 ctatactact atttatgatc ttattatatt gaatattaaa gatatttttc ttagatcttc
     1081 ttagacttgt aataaaaggt atcattaagt ctgctaactc tgtagtagaa gataaatttt
     1141 ctggtatata gataagtttg tttagtaata aaactagagg ttgaccatta tagcctacat
     1201 atttaccggc taattcagtc tttgcttcct cagcactcat cattttaaag tcaatacctg
     1261 tagatataga ggcttgagca agggtattta aaatttctgt atttcttatt ttgttaataa
     1321 gatctacagg atctatctta acaaaatctg caccttgtgg tactttttca agttcctcaa
     1381 taggtgtatc tttatacttt tcttgctctt gtttctctag tatttcctga acatcagcct
     1441 caaatttatc ataatagtct
//
LOCUS       DAOSAB010000154         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_60118_length_1453_cov_3.177396, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000154 DAOSAB010000000
VERSION     DAOSAB010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60118_length_1453_cov_3.177396"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PK432_02810"
     CDS             complement(<1..296)
                     /locus_tag="PK432_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59888.1"
                     /translation="MKNLFGYLKKILLLSSLVFLFSLFSTQKISAQQPNVLSSFQHYW
                     DGETVNTTVLLTFQTTDSPIVITYYTVTIPDTEISPEIFSVSRNKELEFTIHKK"
     gene            complement(250..984)
                     /locus_tag="PK432_02815"
     CDS             complement(250..984)
                     /locus_tag="PK432_02815"
                     /inference="COORDINATES: protein motif:HMM:NF018288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HOK59889.1"
                     /translation="MARIDLKQSSGLPITYIGDDLLPQGLTVSEVSTICINDIRPQLL
                     NEDLDCPEIFYKKYKDIDKDNVFKKKGIKINMYLIYANLAGIEYVKTFATRCKKYPRI
                     LEITYGSGTILLQKYESQTNNRVIKLTVKKGDKIIVPAGYTCSIINSRQNSNLIVLEI
                     HSRKAKPRIVLDDKRGMSYYIIRKNAKQEIVRNPEYKMVEEPEKLDMSKLLSKYGIRN
                     GTPIVKQIMRKYEKFIWLFKEDSVTI"
     gene            complement(1010..1297)
                     /locus_tag="PK432_02820"
     CDS             complement(1010..1297)
                     /locus_tag="PK432_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOK59890.1"
                     /translation="MRFIFKLLYIVITFAETVIIFRIVMSLIGANQANSFVNWIYSMS
                     DVLISPFRGIVADEVYIDKLRIELTPIVSLIFYAIIAFILSELSKTLKKTD"
BASE COUNT          449 a          222 c          223 g          559 t
ORIGIN      
        1 tcttttttat gaattgtaaa ttcaagctct ttatttctac taacagagaa tatttctgga
       61 gatatctctg tatcaggaat tgttactgtg tagtatgtaa ttacaatggg gctgtcagtt
      121 gtctggaatg taagaagtac tgttgtattt actgtttctc cgtcccagta gtgctgaaat
      181 gaggatagca cattaggttg ttgtgctgaa attttttgtg tactgaaaag agagaataga
      241 aatacaagac tagatagtaa cagaatcttc tttaaataac caaataaatt tttcatattt
      301 tctcattatt tgttttacaa ttggtgtacc gtttcttatc ccatactttg acagtagttt
      361 gctcatatca agcttctcag gttcttctac cattttgtat tcaggatttc taacaatttc
      421 ctgttttgca ttttttctaa ttatataata ggacattcct cttttatcat caaggacaat
      481 tcttggttta gcttttctag agtgtatttc aagaacgata agattagaat tttgtcttga
      541 gttaattata gaacatgtgt atccagcagg aacaattatt ttatctcctt tcttaactgt
      601 taattttata actctgttat tagtttgaga ttcatatttc tgaagtagta ttgttccact
      661 tccatatgtt atttcaagta ttctagggta ttttttacat cttgttgcaa atgtttttac
      721 atattctatt cctgcaagat tagcatatat taaatacatg tttattttta ttcctttctt
      781 cttaaacaca ttatctttat caatatcctt gtacttttta tagaatattt caggacagtc
      841 taaatcttca ttaagaagtt gtggacgaat atcgttaata cagattgttg atacttctga
      901 tactgttaat ccttggggca aaagatcatc tccaatatat gtaataggca acccactgga
      961 ttgcttaagg tcaattcttg ccatatgagt ggattatacc ataaagggat taatctgtct
     1021 ttttaagtgt ttttgataat tcagatagaa taaaggcaat aatagcatag aaaattagag
     1081 aaacaattgg tgtaagctct atccttaatt tatcaatata tacctcatct gcaacaatac
     1141 ccctgaatgg agaaattaaa acatcagaca tactatatat ccagttaaca aaagaattag
     1201 cttgattagc tcctattagg gacataacaa tgcgaaagat tattactgtt tctgcaaatg
     1261 ttattactat gtaaagtagt ttaaaaatga atctcatatc tttttgttaa ttaagattat
     1321 ctacataata ttttagctct ctcatatatc ttgagacacc accagaccat acatgagcag
     1381 gattaagcca gcatggaggg cagtagtact gttcaatttg aaaaggtgtt ttatcggttc
     1441 catatccctc tgc
//
LOCUS       DAOSAB010000155         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_60229_length_1452_cov_2.057981, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000155 DAOSAB010000000
VERSION     DAOSAB010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60229_length_1452_cov_2.057981"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1452)
                     /locus_tag="PK432_02825"
     CDS             complement(<1..>1452)
                     /locus_tag="PK432_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59891.1"
                     /translation="GPTDLIYYDNGHIALTIPPAGITNAGDGLIIGYKIGNCIKYYTF
                     HILVLPGVPSVNKCDDRFLFAEYVLPTNDPLDEHLVYCDACGSVSPYCADCCDNLGCI
                     KENCDTQPVCANGTCVCLLNGIPVDPLPNGCCPSECAEGIIIPICSTCVSGAIVPAPV
                     CTAPFILNTNTCLCECPPGAKFSVLDPLNPCITPTNCNIPGQSCDPCFNCVEGVCTPI
                     PCSGVPGMINNPIGDGTVDHPCCIDNPCPNCITSLEVNKYCTDVLPRRANVSADATNY
                     RIKFTQIQLNYLPFYSQVDCEYEALQAITQTNNLIGSVTYEINYTNYVLDWVPVVPSG
                     TTLIIPQSAGNGFLIKCSWKGREVIYEFIFEEGFNGSADQLILGEYPIQVRKVKDLTC
                     GNVYGLSGDCQTTYSWEFTQAELSAAGQFITDNPIVVSPTGLDAEVCVNVTTQSNGVI
                     CEETQICEDVDECQGACGCDPCNGGGTLFSTIE"
BASE COUNT          475 a          302 c          214 g          461 t
ORIGIN      
        1 tgctctatag tactaaataa agtacctcca ccattacatg gatcacatcc acaagcacct
       61 tgacattcat ctacatcttc acatatttga gtttcttcac atataactcc attactttga
      121 gttgttacat taacacatac ttcagcatct agtccagtag gactaactac aataggatta
      181 tctgtaataa actgtcctgc agcacttaat tcagcttgag taaactccca tgaatacgta
      241 gtttgacaat ctccacttaa tccatacaca tttccacaag tcaagtcttt aacttttcta
      301 acttgtatcg ggtattcacc aagtattaat tgatctgcac ttccattaaa cccttcttca
      361 aatataaatt cataaatcac ttctctgcct ttccaactac atttaatcaa aaagccgtta
      421 ccagcacttt gtggaattat taaagtagta cctgaaggca caactggtac ccaatcaagt
      481 acatagttag tataatttat ttcgtatgtt acactgccaa taaggttatt tgtctgtgta
      541 attgcttgta atgcttcata ttcacaatca acttgagaat aaaatggtaa gtaattaagt
      601 tgaatttgtg taaatttaat acgataattc gttgcatctg cactaacatt agcacgtcta
      661 ggtaatacat cagtacaata tttattgact tctagactag ttatacaatt aggacaagga
      721 ttatcaatac agcacggatg gtcaactgta ccatctccaa taggattatt tatcatacct
      781 ggcaccccac tacatggaat tggagtacaa actccttcca cacaattaaa acatggatca
      841 cagctttgtc ctggaatatt acaattagtt ggtgtgatac acggatttaa tggatctagc
      901 acactaaatt tagcccctgg agggcattca cataagcacg tattagtatt taatataaat
      961 ggtgctgtgc aaacaggtgc tggtacaata gctccgctta cacaagtaga gcatattggt
     1021 ataataattc cttcagcaca ttcactagga caacatccat taggtaatgg atctactggt
     1081 attccattca gtaaacaaac acatgtgcca ttagcacata ctggttgagt atcacaattt
     1141 tcttttatgc agcctagatt atcacagcaa tctgcacaat aaggactaac acttccacac
     1201 gcatcacagt acactaaatg ttcatcaaga ggatcgttgg tcggtaatac gtactcagca
     1261 aacaaaaacc tatcatcaca tttattcact gatggaacac ctggaagtac taatatgtga
     1321 aacgtatagt acttgataca atttccaatt ttatatccta ttataagtcc atcacctgca
     1381 tttgttatac ctgctggagg tattgttaat gctatatgtc cgttatcata atatataagg
     1441 tcagttggac ca
//
LOCUS       DAOSAB010000156         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_60273_length_1451_cov_2.638252, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000156 DAOSAB010000000
VERSION     DAOSAB010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60273_length_1451_cov_2.638252"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PK432_02830"
     CDS             <1..816
                     /locus_tag="PK432_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59892.1"
                     /translation="NYLSISFLDDKTKIYLNGELSFSGDSYALNANQYKIGLGCSDVQ
                     DVFNGEIGNVRYSTVVQLEGDILDYHTIEERKYLLKPVFRADLQNSSQILNAQSTSFS
                     ITQLPYGADNYVVSLNKGDTIVVVQDEYTASGVVSSVDNDTGLVTVNEWDSSSTFPAD
                     GFSTSADVLKWQREYIPGVLGDSFNVQYSGDLQIKDMSLISAFNSGESINIQEGGKYV
                     KYRLIFISKYPDISASISSVTINIEESGPQMEQIMRHGKWFNESGKQPYWWAQ"
     gene            834..>1451
                     /locus_tag="PK432_02835"
     CDS             834..>1451
                     /locus_tag="PK432_02835"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK59893.1"
                     /translation="MSDFKDALGENKKFLLLALALLAVCALVVFSLQSGKYPSGTLII
                     DKGKTYSAAERVLDEKYDYSIIVSTVYGDIFIDLYEKEAPDNVNSLLFLISKRYYEGL
                     TFHKVVKDFVIQAGDTKGDGSGDPGYTVKRENLVDFNDYDVGMANASQFFIVLPNSNK
                     QKFNGEYTLVGKVIKGFAVVDSIAKVEVDEDYKPVNDVVIKSILIQ"
BASE COUNT          475 a          177 c          326 g          473 t
ORIGIN      
        1 aattatcttt ctatttcttt tttagatgat aaaacaaaga tatatttaaa tggtgagctt
       61 tcatttagtg gtgattcata tgcattaaat gccaatcagt ataaaattgg tctaggatgc
      121 agtgatgtac aggatgtatt taatggtgag attggaaatg tgagatattc tactgttgta
      181 caactggaag gagatatctt agattaccat acaattgaag aaagaaaata tttgcttaaa
      241 cctgtattta gagctgattt gcagaatagc agccagatat taaatgctca aagtacctct
      301 ttttcaatta cacaactccc ttatggtgct gataactatg ttgtaagcct taataaaggg
      361 gatacaattg ttgttgtgca ggatgagtat acagcttctg gtgttgtgag ctctgttgac
      421 aatgatacag ggcttgtaac tgtaaatgag tgggacagca gtagtacatt tcctgcagat
      481 gggtttagca catctgcaga tgttcttaaa tggcagagag agtacatacc tggagtttta
      541 ggagatagct tcaatgtaca gtacagcggg gatttacaaa ttaaggatat gagcttaata
      601 tctgcattta atagcgggga gagcattaat atacaggagg gtggtaaata tgttaaatat
      661 aggctcatat ttatatcaaa ataccctgat atatctgcat ctatctcaag tgtcacaatc
      721 aatattgaag aaagcggccc acagatggag cagataatga ggcatggaaa gtggtttaat
      781 gagagtggta aacagccgta ctggtgggct caataatgtg ttaaaatatt gaaatgagtg
      841 actttaaaga tgcattgggg gagaataaaa agtttctgct tttggcattg gcacttcttg
      901 ctgtttgtgc tttagttgtt ttttccctgc agtcaggaaa atatccatct gggacactaa
      961 ttattgataa aggaaaaaca tattctgcag cagaaagggt cttagatgag aaatatgatt
     1021 acagcattat tgtttctaca gtatatggag atatatttat agatctgtat gaaaaggagg
     1081 ctccggataa tgttaatagc ttactgtttt tgattagtaa aagatattat gaagggctta
     1141 catttcataa ggttgttaag gattttgtaa ttcaagcagg ggatacaaaa ggagatggaa
     1201 gtggagatcc tgggtataca gtaaaaaggg agaatctagt agattttaat gattatgatg
     1261 taggaatggc taatgcttct caatttttca tagttcttcc taactctaat aaacaaaagt
     1321 ttaatggaga gtatacttta gttgggaaag ttattaaggg ttttgcagtt gttgactcta
     1381 ttgccaaggt agaagttgat gaagactata agcctgttaa tgatgtggtt attaaaagta
     1441 tattaattca a
//
LOCUS       DAOSAB010000157         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_60555_length_1447_cov_1.558908, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000157 DAOSAB010000000
VERSION     DAOSAB010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60555_length_1447_cov_1.558908"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PK432_02840"
     CDS             <1..290
                     /locus_tag="PK432_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59894.1"
                     /translation="QINNKGEISPYFGIIVKNIGNVNYKEVVDDEESLDSNKYLKYSG
                     VALSDLTTKIQFDVNIKFVNGREYKRTFKGLLDGEMFAQSSTPVIKLEVIE"
     gene            complement(280..1431)
                     /locus_tag="PK432_02845"
     CDS             complement(280..1431)
                     /locus_tag="PK432_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOK59895.1"
                     /translation="MRKIYKIFLTAIFLLILLILLPPPKNNNLIYDIQGDIQIKKTPP
                     LEERILSQMTVEEKIGQLLLFGFSGTSLNADIQNLIETKYIGGVLLLGKNIQNMQQLK
                     KLNNDLQSISQLPLFIAVDQEGGSVARIQESSKTNISQRYLKDEQQIYDISVQRAKML
                     KELGINMNLAPVAEYITNNSSFMYERTFSGSRQEVAQKVYSAVKGFVDSKMISTIKHF
                     PGHSDQSKDTHSTIAQVNISPQQWDEYIYTFKYPIDKGIVDVIMVGHIKFPKIDDKPA
                     TVSKVIIDEKLRKEIGYEGVVITDDMQMSSIWKYAQYCNTAKEALLAGNDILLYSQYT
                     PRATLLTDVYNCILNAITSGEIAEDVINQKVLRILKLKIKYGLIDETTL"
BASE COUNT          423 a          219 c          232 g          573 t
ORIGIN      
        1 atcaaattaa taacaagggt gaaatttctc catattttgg aattattgtt aaaaatatag
       61 gcaatgtaaa ttacaaagag gttgttgatg atgaggaaag tttagatagt aacaaatatc
      121 ttaagtacag tggtgttgca ctttcggatt taacaactaa aattcagttt gatgttaata
      181 ttaagtttgt aaatgggaga gagtataaaa ggacatttaa aggattgctt gatggtgaga
      241 tgtttgcaca gagcagcacc cctgttatta agcttgaggt tatagagtag tttcatctat
      301 taatccatat tttattttta gttttaatat tcttaaaacc ttttgattta ttacatcttc
      361 agctatttct ccacttgtta tagcattcag tatacagttg taaacatctg ttagaagagt
      421 tgctcttggt gtgtattgag agtaaagcaa gatatcattt cctgctagta gtgcttcttt
      481 agcagtattg cagtactgtg catatttcca aattgagctc atctgcatat catcagttat
      541 tactactcct tcgtacccta tctcttttct tagtttctca tcaattatta cttttgatac
      601 tgttgcaggt ttatcatcaa tctttggaaa tttaatatgt cctaccatta ttacatcaac
      661 aatacccttg tctattggat atttaaatgt atatatgtat tcatcccatt gttgtggtga
      721 aatatttacc tgtgcaatag tagagtgtgt gtctttagat tgatcactgt gtccagggaa
      781 atgctttatt gtacttatca ttttagagtc cacaaatcct tttactgcag aataaacttt
      841 ctgtgctact tcttgtctgc ttccactaaa ggttctttca tacataaatg aggagttatt
      901 tgtaatatat tctgcaacag gtgctaggtt catgtttata cccagctctt taagcatctt
      961 agccctttgt acagagatgt catatatttg ctgttcatct ttaagatatc tttgagatat
     1021 atttgtttta ctgctctctt gtattcttgc tacacttcct ccttcttgat cgactgcgat
     1081 aaatagagga agctgcgaaa tactttgtaa atcattattt aatttcttta actgctgcat
     1141 attttgtata ttttttccta acaaaagtac tccccctata tattttgttt ctattaaatt
     1201 ttgtatatca gcattaagtg aagttccgct aaaaccaaaa agaagtagct gtccaatttt
     1261 ttcttcaact gtcatttgag aaagtattct ttcttccagt ggaggagtct ttttaatttg
     1321 tatatcacct tgtatatcat aaatgaggtt attatttttc ggtggtggta aaagaatgag
     1381 aagtatgaga agaaatatag ctgttaaaaa tatcttgtat atttttctca tatatacata
     1441 ttatcat
//
LOCUS       DAOSAB010000158         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_62804_length_1411_cov_3.441740, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000158 DAOSAB010000000
VERSION     DAOSAB010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62804_length_1411_cov_3.441740"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PK432_02850"
     CDS             <1..441
                     /locus_tag="PK432_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOK59896.1"
                     /translation="LSRIINIGGIILITTMIIVTIALIRVTIGFNIKVHQEEIEIMQL
                     VGSSDKFIKTPFLLEGTFYGIAGGFLASLIVLIPWYATNIYSSGTDFSIWITQMFKDI
                     NLEYVLKPNFTFILVFILIHIIIGSLIGFLSSLSSVKKYLKEET"
     gene            438..>1411
                     /locus_tag="PK432_02855"
     CDS             438..>1411
                     /locus_tag="PK432_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOK59897.1"
                     /translation="MKNLKKIFTITISIIFLFIVSKDVRAAEACPESMPAMDRYLCLQ
                     KQLSKLESNQNNLQKKLKNEDYQQLTLNEKLKYIDTQIQQTESVIETLGIEIAAKDIE
                     INMLNEEVTAKENDLSTLRQEINALEQTVGERVIQSYKYSHVGPFEVLLDFRGFDNVI
                     RKLKYLLETRTNDKNSLAAYADKKDLLEKEQAILTAQQIELQNKRAEVEEEKKRLAEE
                     KKYLDEQKAEKQRLLAESQRREEAYRKEIAETTAMISQIDEMISDVVIQLFNSNQLGN
                     GSKVSAGTVIGFQGHTGCAFGSHLHFEIRNKKDVKQDPSLYLGGGEYWS"
BASE COUNT          567 a          183 c          253 g          408 t
ORIGIN      
        1 ttatcaagga taattaacat aggtggaata atattaatca ctacaatgat aatagttaca
       61 attgcattaa ttagagtaac aataggattt aatattaaag ttcatcaaga agaaatagag
      121 ataatgcagc ttgtaggaag ttctgacaag tttataaaaa caccttttct tctagaagga
      181 accttctatg gtattgctgg agggttttta gcttctttaa tcgttttaat cccttggtat
      241 gctacaaata tatattcaag tggtacagat ttctcaatat ggataactca aatgtttaaa
      301 gatattaatt tagaatatgt tctaaaacct aattttactt ttattcttgt atttatatta
      361 atccatataa taataggatc attaatagga tttcttagta gcttaagctc tgttaagaaa
      421 tatctaaagg aggaaacatg aagaatttaa aaaagatatt tacaattaca atcagtatta
      481 tatttctctt tatcgtatcc aaagatgtta gagcagctga agcctgccct gaaagtatgc
      541 ctgcaatgga cagatacttg tgtcttcaaa agcaactaag caaattagaa agcaaccaga
      601 ataatctaca aaagaaactt aagaatgaag attatcagca gttaacttta aatgagaaac
      661 ttaaatatat tgatactcaa atacaacaga cagagagtgt tatagaaaca ttaggtattg
      721 aaattgctgc aaaagatatt gaaatcaata tgcttaacga ggaggtaact gcaaaggaaa
      781 atgatcttag tacattaaga caagagataa atgcacttga gcaaactgta ggagaaagag
      841 ttatacaatc atataaatat tcacatgttg gaccttttga ggtattactt gattttagag
      901 gttttgacaa tgttataaga aaattaaaat atctacttga aacaagaaca aacgataaga
      961 actcccttgc agcatatgct gataaaaaag atttgctcga aaaagaacaa gctatactaa
     1021 cagctcagca aattgaacta caaaataaaa gagcagaggt agaggaagaa aagaaaagac
     1081 ttgcagaaga gaagaaatat cttgatgaac aaaaggctga aaaacaaaga cttctagcag
     1141 aatcacaaag gagagaggaa gcatatagaa aagagatagc agagacaact gcaatgatat
     1201 ctcagattga tgaaatgatt tctgatgtgg taattcaact atttaattct aaccaattag
     1261 gaaacgggtc aaaggtaagt gctggaacag taataggctt tcaagggcat acaggatgtg
     1321 catttggttc acatttacac tttgaaatcc gtaataaaaa ggatgttaaa caagatcctt
     1381 cattatattt aggtggggga gaatactggt c
//
LOCUS       DAOSAB010000159         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_63409_length_1402_cov_2.625835, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000159 DAOSAB010000000
VERSION     DAOSAB010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63409_length_1402_cov_2.625835"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..349
                     /locus_tag="PK432_02860"
     CDS             134..349
                     /locus_tag="PK432_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59898.1"
                     /translation="MREKYKEEIKIMPGVVKVLVALVILIAISLLVRKIFVTKKESLE
                     FVDGTLEGVSFLILTLFLLLVLIIVFC"
     gene            506..1309
                     /locus_tag="PK432_02865"
     CDS             506..1309
                     /locus_tag="PK432_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOK59899.1"
                     /translation="MARTKKEVKKKVESKPKEEEKEIVIDIDKIILPLSIFLAGLLIA
                     LSIFLTNNNGSNGNDTTGSDAAGTTDTTDTAAQDEFPAAKTVIGNSPYLGDIKKAKVA
                     VVEYTDLQCPYCQRHATETEPQIVSEYVDTGKIIYVVRSFPLESLHGELATQSANAVL
                     CVNELGGRDKFYEFYKQAFAKASVEELAQVAQSVGVDMNKYNTCMSENRYKDQIDKEI
                     QDGTDAGIQGTPGFVIGVLDKDGNVDGKMIAGAYPYESFKAIIDEMLAK"
BASE COUNT          468 a          172 c          270 g          492 t
ORIGIN      
        1 agtttttact cttcatatat atttacagga cttctaatct agatttttat ttcattgtat
       61 tatttacttt ggttacccta tagtaatttg tttatagatt tagtataatg tatatgtacc
      121 ttgttcatta aaaatgagag aaaaatataa agaggagata aagataatgc ctggagttgt
      181 gaaagttttg gttgcattgg ttattttgat agcaatctca cttcttgtga gaaaaatctt
      241 tgttacaaaa aaagagtctt tggaattcgt tgatggaact cttgagggag tttctttttt
      301 aattcttact ttgtttcttc tgttggttct gattattgtg ttttgctaat taactaatta
      361 acaaggtaca gagaagtcct gtgaattgaa tgagttttta ctcttcatat atatttacag
      421 gacttctatc ttaatttatt gacatattta tatatttaaa atatcattat ccacatatat
      481 taattaatat ttaaaacata tatacatggc aagaacaaag aaagaagtta agaaaaaggt
      541 tgaaagtaaa cctaaagaag aagagaaaga gatagtaata gatattgata aaataatatt
      601 accactatca atatttctag caggtctttt aatagcatta tcaatatttc taacaaataa
      661 taatggaagt aatggaaatg atactactgg aagtgatgca gctggtacaa cagatactac
      721 agatactgct gcacaggatg agtttcctgc tgctaagact gttattggga acagtccata
      781 tttaggggat attaaaaagg ccaaggtagc tgttgttgaa tatactgact tacagtgtcc
      841 ttactgtcag agacatgcta ctgagacaga gcctcagatt gtatctgagt atgttgatac
      901 tggtaaaatt atatatgtag taagaagttt cccattggaa agcttacacg gagagcttgc
      961 aacacagagt gctaatgcag ttttatgtgt taatgagctt ggtggaagag ataaattcta
     1021 tgagttttac aaacaggctt ttgctaaagc atctgttgag gaacttgctc aggttgcaca
     1081 aagtgttggt gtagatatga ataaatacaa tacatgcatg agtgagaaca gatacaagga
     1141 tcagattgat aaagaaatac aggatggtac agatgcaggt atacagggta ctccagggtt
     1201 tgtaattggt gtacttgata aagatggcaa tgtagatggg aagatgatag caggtgcata
     1261 tccttatgaa tcattcaaag ctattattga tgagatgtta gctaagtaag agtattgttg
     1321 attaattaaa aggggaagtt atcttcccct tttttattta tacatcttct tgattacttc
     1381 ttctttttct ttacctctgt tt
//
LOCUS       DAOSAB010000160         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_63739_length_1397_cov_2.462742, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000160 DAOSAB010000000
VERSION     DAOSAB010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63739_length_1397_cov_2.462742"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PK432_02870"
     CDS             complement(<1..299)
                     /locus_tag="PK432_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59900.1"
                     /translation="MQTGEDYFKDTTDDFSSGGGSFRSIRSSKTDRTDAIEKIFMDVL
                     GRKPSSREMAYYKYSPIKEEDIITKLISSDEHKKIVESALKLPGVQEELKNVRIS"
     gene            complement(338..410)
                     /locus_tag="PK432_02875"
     tRNA            complement(338..410)
                     /locus_tag="PK432_02875"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(375..377),aa:Gly,seq:tcc)
     gene            complement(421..840)
                     /gene="rplS"
                     /locus_tag="PK432_02880"
     CDS             complement(421..840)
                     /gene="rplS"
                     /locus_tag="PK432_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOK59901.1"
                     /translation="MNSEILEKVEKDQYKKRPDVRVGDTVKMYLKIKEGNKERVQVFQ
                     GVVIGIKGSGLSKTVTVRKISYGVGVEKIIPLYASTLQKIEIVKRGKVRRSKLYYLRS
                     RVGRRALKVAKSEGMYETDELEVPIEGEKEDAEIAEK"
     gene            928..>1397
                     /locus_tag="PK432_02885"
     CDS             928..>1397
                     /locus_tag="PK432_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOK59902.1"
                     /translation="MEERKKVLLIIMDGLGAAPAGKGNAVVLANPQNLSSLWTTSPHT
                     YLLASGEAVGLPNNAKGNSEVGHMNIGGGRVVLQTLPRINKSIEKGLYFNNNILKEAL
                     DFANKNKSRVHILGLASQGGVHADTGHITATIEFFSKRNFQGDIFIHAFTDGRDS"
BASE COUNT          447 a          286 c          202 g          462 t
ORIGIN      
        1 tctgatattc tcacattctt aagctcctct tgaacaccag gaagcttaag tgcactctct
       61 acaatctttt tatgctcatc cgaactaatt agttttgtaa taatatcttc ttctttaata
      121 ggagagtatt tgtaatatgc catctccctg ctacttggct ttctaccaag tacatccata
      181 aaaatctttt caatagcatc tgtcctatct gtcttagaag atctaataga acgaaaagaa
      241 ccaccacctg aactaaaatc atctgttgta tctttgaaat agtcttctcc tgtttgcata
      301 tctgattata ccgtaaacaa gtggaaaaat cagtaaaagc gggagacggg actcgaaccc
      361 gcaaccctct gtttggaaaa cagacattct aaccattgaa ctactcccgc acagcaagca
      421 ttacttctct gctatttcag catcttcctt ctcaccttca ataggtactt ctagttcatc
      481 agtttcatac atgccttcac tttttgcaac tttaagtgcc cttcttccaa cacgacttct
      541 taaatagtaa agctttgatc ttctaacttt acctcttttt acaatctcaa ttttctgtaa
      601 tgtactcgca tacaaaggaa ttatcttttc tacacctact ccatatgata tttttcttac
      661 agttacagtc tttgataatc cactcccttt aataccaata acaacaccct gaaatacctg
      721 tactctctct ttgttcccct ctttaatttt taaatacatc ttaacagtat ctccgactct
      781 tacatcaggt ctctttttgt attgatcttt ctcaactttt tctaatatct cgctattcat
      841 agtagtataa tgtaaaatat ttaactaatt ctgaaaggta ttatagaata aaataataaa
      901 ttatgccaga atatatataa aactgccatg gaagagagaa aaaaagtact gctaataata
      961 atggatgggc ttggagctgc gcctgctggc aaaggaaatg ctgtagtact tgctaatccc
     1021 caaaatcttt catcattatg gacaacttca cctcatacat atttattagc ttctggtgag
     1081 gctgtaggac ttcctaataa tgcaaaagga aacagtgagg taggacatat gaacatagga
     1141 ggaggaagag tagtacttca aacacttcca agaatcaaca aatctattga aaaaggtctg
     1201 tattttaaca acaacatatt aaaagaagca ctagattttg caaataaaaa taaatctagg
     1261 gtgcatatac ttggtttagc aagtcaggga ggtgtacatg ctgatacagg acatatcaca
     1321 gcaactattg agtttttttc aaagcggaat tttcaaggag atatctttat acatgctttt
     1381 actgatggaa gagattc
//
LOCUS       DAOSAB010000161         1396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_63800_length_1396_cov_2.420582, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000161 DAOSAB010000000
VERSION     DAOSAB010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63800_length_1396_cov_2.420582"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PK432_02890"
     CDS             complement(<1..938)
                     /locus_tag="PK432_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOK59903.1"
                     /translation="MTETVKSPQELEKEFYSKPLYLSYSGLNKIVFSPKLYYKHYILQ
                     QREEKLDSYLIDGKVIHNLLLDDGSFEKNFILLPSTLPTGNSRLVIDKIFEKHKEHVL
                     SSFGTIGTLDDFGKDILEILKQINLHQSLKTDAQRFAKIITEENISYFEFLKLKGSKD
                     LIDSETLQRCNEAVDVLKNDSKVTDLLGTYRSEMENTKIYNELELSLESSVLGRAFGL
                     KGIIDNIKVDYDNKTVYINDLKTTGKTISDFKETIEYYNYNLQAAIYHKLVKNMLKDI
                     MNADWNIVFNFIVVDKYTQVYPFEVSKVTMAIWLDI"
     gene            complement(935..>1396)
                     /locus_tag="PK432_02895"
     CDS             complement(935..>1396)
                     /locus_tag="PK432_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59904.1"
                     /translation="QGVNNLFNSNDTHDHRVAMEAMANCDYQKSAVYLLMLFYYNHKL
                     IYNCDSKTHVNFKSFLKFFNLNATHGIDIDDIIERLKDKKLLTRSNLNIVMAKAKELL
                     DESATNVSTYFVPTGIAPIEEIIKEVEETDSLQTPAIPVADPMTETQITDL"
BASE COUNT          418 a          243 c          200 g          535 t
ORIGIN      
        1 aaaatatcaa gccaaatagc cattgtaact ttacttactt caaaagggta aacttgtgta
       61 tacttgtcta ctactataaa gttaaaaact atattccaat cagcattcat tatatccttt
      121 agcatgtttt taacaagctt atgataaatt gctgcctgaa gattgtagtt atagtactca
      181 atagtttctt tgaagtctga gatggtttta ccagtagtct taaggtcatt gatgtaaacg
      241 gttttgttat cataatctac cttaatatta tctataatac ctttcagtcc gaaagctcta
      301 cctaatacac tactctctag agacagctct agttcattgt atattttagt gttttccatc
      361 tctgatcggt atgtcccaag aagatcagtt actttactgt cattcttaag tacatctact
      421 gcttcattac atctttgtag tgtctcgcta tctattaggt ctttactacc ttttagcttt
      481 aagaactcaa agtaactgat attttcctct gttataattt ttgcaaatct ctgtgcatct
      541 gttttaagag attggtgtaa gttgatctgt ttaagtatct ctaaaatatc tttaccaaaa
      601 tcatccagag tacctattgt tccaaaagaa ctaagaacat gctccttatg tttttcaaat
      661 attttatcaa taactagtct tgagttgcct gtgggtagag tagatggcaa aagtatgaaa
      721 tttttttcaa aactaccatc atcaagcaac aaattgtgaa taacttttcc atctattaaa
      781 tagctgtcaa gtttctcttc cctctgttga aggatgtaat gtttataata gagcttaggt
      841 gagaatacta ttttattcaa gcctgaataa cttaagtaca aaggcttgct ataaaactct
      901 ttctctagct cttgcggtga ttttactgtt tctgtcatag atctgttatt tgtgtctccg
      961 tcataggatc agccaccggg attgcaggag tctgtaaact atctgtttct tctacttctt
     1021 ttataatctc ttctattggt gctatacctg ttggtacaaa atatgtactt acattggttg
     1081 cgctctcatc taatagttct ttagcttttg ccattactat attaagattg cttcttgtaa
     1141 gtaatttctt gtcttttaat ctttctataa tatcatcaat atctatacca tgagtagcat
     1201 taagattaaa gaacttgaga aaactcttaa agttaacatg agttttgcta tcacaattat
     1261 agattaactt atgattataa tagaagagca tcaatagata tacagctgat ttctgataat
     1321 cacaattggc catagcttcc atagctactc tatgatcatg agtatcatta ctattaaaaa
     1381 gattatttac tccctg
//
LOCUS       DAOSAB010000162         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_64555_length_1384_cov_2.661400, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000162 DAOSAB010000000
VERSION     DAOSAB010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64555_length_1384_cov_2.661400"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..>1384)
                     /locus_tag="PK432_02900"
     CDS             complement(23..>1384)
                     /locus_tag="PK432_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59905.1"
                     /translation="QYPDLVSQITQRGGLGFLNKSTHTMGTGRWSSSNPKNMFMNVLK
                     QAYQNASTQAPVSTEVKGNIQLELIEDLVQQGVAKTTVRNYDRPSGIYKSAKTENLYN
                     LVNRGKVKMVGDKIVGKGISYTLDEFGAAEGFGNWAGFTKAAKYAGKDLIAGKEVFLY
                     DITAAGKKADKARPVFDSLPGKSSASTMTYAGIGSRQTPQEVLDKMTEVAKYLDDLGY
                     TLNTGKTYPTKLNDYLIKTVHKGNKEAAEKAYKNKQAETERLSKLYGNTVGMDEEGAD
                     RAFSAGASKKNLFSPSDKIGQKEMKVGEEIHPNWSALSAGAAKLMARNTNQVFGENLD
                     KPVDFVLFYAKEVKGSIRPEGGTGQAVEMARRKGIPTINMADENWRDQLKAIIKQRPT
                     QQPLDFKTMPEFSLERKREILSNFANKHNITSDQAYQYINQALNDPSKKKEDIIKKLK
                     ECY"
BASE COUNT          384 a          280 c          194 g          526 t
ORIGIN      
        1 atttggattt acacatgcca tcttaataac actcttttag ttttttaata atatcttctt
       61 tcttcttact tggatcattt aatgcttggt ttatgtactg ataagcttga tccgaagtaa
      121 tattatgttt attagcaaag ttacttaata tttctctttt tctttctaag ctaaattcag
      181 gcatagtttt aaagtctaaa ggttgttgag taggcctttg ttttataatc gcctttaatt
      241 gatctctcca gttctcatca gccatgttga tagtaggtat accttttctc ctagccattt
      301 ctactgcttg tcctgtaccg ccttcaggtc tgatagagcc tttaacttcc ttggcataga
      361 ataaaacaaa atctacaggt ttatcaaggt tctcaccaaa tacttggtta gtatttctag
      421 ccatcaactt agctgcaccc gctgataaag ctgaccaatt aggatgaatt tcttcaccta
      481 ctttcatttc tttttgccct atcttatcgg aaggactaaa taaattcttt ttacttgctc
      541 cagcagagaa agccctatca gcaccttctt catccatacc aacagtattg ccatatagtt
      601 tactcaatct ttctgtttca gcttgcttat tcttataagc tttttcagca gcttctttat
      661 tacctttatg tacagttttt ataaggtaat catttaactt ggtaggataa gttttaccgg
      721 tattcaaagt ataacctaaa tcatctagat attttgcaac ctcagtcatc ttatcaagaa
      781 cttcttgagg agtttgccta ctaccaatac ctgcataagt catggtagat gcactggatt
      841 taccaggtaa agaatcaaat actggtctag ctttatcagc ttttttacca gcagctgtta
      901 tatcatataa aaatacttct ttacctgcta tcaaatcttt acctgcatac ttagctgctt
      961 tagtaaagcc tgcccagtta ccaaatcctt cagctgcacc aaactcatca agtgtataag
     1021 atataccttt acctactatt ttatcaccta ccatttttac tttaccacgg tttactaagt
     1081 tatataagtt ttcggtttta gcagatttgt atataccaga tggtctatca taatttctaa
     1141 cagtagtttt agctacacct tgttgtacaa gatcttctat aagttctagt tgaatattac
     1201 ctttaacttc tgtacttaca ggggcttggg tagatgcatt ttgatatgct tgttttaaaa
     1261 cattcataaa catattctta ggattactgc tactccatct acctgtaccc attgtatgag
     1321 tagatttatt taagaagcct aacccacctc tttgagtaat ctgagataca agatccggat
     1381 attg
//
LOCUS       DAOSAB010000163         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_68592_length_1329_cov_2.527473, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000163 DAOSAB010000000
VERSION     DAOSAB010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68592_length_1329_cov_2.527473"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PK432_02905"
     CDS             complement(<1..1072)
                     /locus_tag="PK432_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOK59906.1"
                     /translation="MKLKYKFKNKYHNITYNVYETENGIKVLYLDNPATVDFDFAILF
                     KAGTSFETKENVPNGTAHFLEHMLLNPNSTFKTKEEIDKFEQGSKTRPALDINASTTR
                     KNIYITSHTNQKGYLRAMERIESILEFPKRKFASQMEKERGIILAEKSRKLKKEKDSF
                     FQSLIFLFNNEQKEFTYDILGEEGDIKRISIDDLEKYFKSRFVTGNCVFAIQAKGEMN
                     SKVKSRLEQISKKIPYGIEDIPNKPMLKNKWKVGCFNDNRANGVSFSLMYFNNAEEKI
                     DYKLDVVQFLTSKLLAWLAFDVLREKKSLIYSFSPFKAGDLSFYYDISGFSFTTEQSK
                     VLDTLNELYTLMHDYTFHFLQTK"
BASE COUNT          414 a          211 c          171 g          533 t
ORIGIN      
        1 ttttagtttg tagaaagtga aaagtataat catgcataag agtatataat tcatttaaag
       61 tatctaacac cttactttgt tctgttgtaa agctaaatcc agatatatca tagtaaaaag
      121 aaagatctcc tgccttaaat ggggagaaac tatatattaa actcttcttc tctcttaaca
      181 catcaaatgc tagccatgcg agtaacttac tagtaagaaa ttgtacaaca tcaagcttgt
      241 aatcaatttt ttcctcagca ttgttaaaat acattaatga aaatgaaact ccattagctc
      301 tattatcatt aaaacagccc actttccatt tgtttttaag cattggctta ttaggtatat
      361 cctctatacc atatggtatt ttctttgata tttgttctaa cctactttta accttgctat
      421 tcatttctcc tttagcttga atagcaaata cacagttacc tgtaacaaat ctacttttaa
      481 aatacttttc taaatcatct atgcttattc tttttatatc cccttcttct cctaatatgt
      541 cgtatgtaaa ctccttttgt tcattgttaa aaagaaatat taaactttga aaaaaggaat
      601 ctttttcttt tttaagtttt ctgctttttt ctgcaagtat aatgcctctc tctttttcca
      661 tctgagatgc aaattttctc ttaggaaatt ctaatatact ctctattctt tccatagctc
      721 tcagatatcc tttttgatta gtatgggatg taatgtatat gttctttctt gtagtacttg
      781 catttatatc taaagctggt cttgttttac ttccctgttc gaatttgtca atttcttcct
      841 ttgttttaaa tgtactattt ggattaagaa gcatatgctc taaaaagtgt gctgttccat
      901 tgggtacatt ctcttttgtt tcaaatgatg tacctgcttt aaaaagtatt gcaaaatcaa
      961 aatctactgt tgctggatta tctaagtaca gtacttttat gccattttct gtttcatata
     1021 cattgtatgt aatattgtgg tatttatttt taaacttgta cttcagcttc atcagtagat
     1081 tctatcatat taatatcaag acttcttaat aggtagagta aatttgacaa gtttacatac
     1141 tttagtgtct caaaaaaaag atatacaaag gaataaagtt tactgtataa ttaagtatgg
     1201 aaattgagaa ggataggaca tttagtcttg agaatactgc cttctcagac caatattttg
     1261 aagactcctt taaaagagtc atagagtttg tttcttcaaa taaagatttc aatatatatg
     1321 atttagata
//
LOCUS       DAOSAB010000164         1319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_69315_length_1319_cov_3.655854, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000164 DAOSAB010000000
VERSION     DAOSAB010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69315_length_1319_cov_3.655854"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PK432_02910"
     CDS             complement(<1..741)
                     /locus_tag="PK432_02910"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOK59907.1"
                     /translation="MAKIKIKKAAPVKTTEKKAPKTVKVPTVKIKKPNIKFQKPDIKK
                     ISSSKGFKTFLTVLGVIVAFILIDLFVQYLNNGYSAAVVEGKRIPRSEYIKELETAYG
                     ATVIDQLIDEEIIKIEAEKAGVEATEEEVNKQLDQIIESVGGKEVYETVLQQNNIDEE
                     ELKNQITLSILANKVLGPTVEYTEDDLKSFFNQYSAQLFPTETEALEEGEKLDYEQYK
                     DQVKQRYIEQKVYENEGAWLEGLRSKYKI"
     gene            complement(823..>1319)
                     /locus_tag="PK432_02915"
     CDS             complement(823..>1319)
                     /locus_tag="PK432_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59908.1"
                     /translation="RVSAGSIIGLQGHTGCAFGSHLHFEIRNKYDVKQDPSLYLGGGE
                     YWSLVTSAKYLAPMRRARITQTYKLSPRPHRAWDMVSTIDGDQSGSTYKVTKGICDNV
                     DYYMEKENREWAFLTGEGAPVKAITSGTVYYGVYSTNLPQYPCKYALLVHDDGNKSFY
                     LHLK"
BASE COUNT          346 a          238 c          184 g          551 t
ORIGIN      
        1 tattttgtat ttacttctta gaccttctaa ccatgcacct tcattttcat aaaccttttg
       61 ttcaatatac ctttgcttta cttgatcttt gtattgttca taatcaagtt tctccccttc
      121 ttctaaagct tctgtttctg tggggaagag ttgtgcacta tactgattaa agaatgattt
      181 cagatcatct tcagtatatt caacagtagg tcctaatact ttatttgcta gtatacttaa
      241 tgtaatttga ttttttaact cctcttcatc tatattattc tgctgtagta ctgtttcata
      301 tacctctttt cctcctacac tttctattat ttgatctaat tgtttgttta cttcctcctc
      361 tgttgcttct actcctgctt tctctgcctc tattttaata atctcttcat ctattaattg
      421 atcaataact gttgctccat atgctgtttc taattcttta atgtattcac ttcttggaat
      481 tctttttcct tctacaacag ctgcactata tccattgttt aaatattgaa caaataggtc
      541 tatcaagata aaggctacta ttacaccaag tactgttaga aatgttttaa atccttttga
      601 agatgatatt tttttaatat ctggtttttg gaatttaata tttggtttct ttatctttac
      661 agttggtact ttaactgttt ttggtgcttt tttttctgtt gttttgacag gagcagcttt
      721 cttaattttt atctttgcca tttaaatatt taaataaaat taatttggct tatagttaag
      781 tttaataagt ttagcagaaa tttatatatt tatcaaaggg ggctatttta aatgcaaata
      841 gaaggactta tttccatcat catgaacgag tagggcatat ttacatgggt attgtggaag
      901 gtttgttgaa tatactccat aatatacagt acctgatgta atagctttaa caggagctcc
      961 ttcaccagtt aggaatgccc attctctatt ctctttttcc atataataat caacattatc
     1021 acagatacct ttagtcactt tataggttga tccactttgg tctccatcaa ttgtgcttac
     1081 catatcccat gctctatgag gtcttggtga taatttatat gtttgtgtga ttcttgctct
     1141 tctcattggt gcaagatatt tagctgatgt tactaatgac cagtattctc ctccgcctaa
     1201 ataaagggaa ggatcttgtt taacatcata tttattacgg atttcgaaat gcagatgtga
     1261 accgaatgca catccagtat gaccctgcag tcctattatt gaacctgcac ttaccctag
//
LOCUS       DAOSAB010000165         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_69793_length_1313_cov_3.329889, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000165 DAOSAB010000000
VERSION     DAOSAB010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69793_length_1313_cov_3.329889"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PK432_02920"
     CDS             <1..1236
                     /locus_tag="PK432_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59909.1"
                     /translation="ISPFTATTPGMPLTNVSLVQSMTNISAASMGLSSTDLIYAITND
                     NQPVWFLSPAAIALLPNNTYLQPYMYFTDTPGTYNKCDINIAAPYTIADVKQTSAQGG
                     QSTCTPIQEIKEKDSCTGLETYRYITEDGTGNLVDINTVIPNFDETKVLLSCPESPIL
                     LEKEVCGTIDGSVDTYELIKVYTRNPTTGVSTILFYEDNKGNQITGAVVESCCTCNTL
                     CDVPTVIVPVPNQRCLGYSSNNDPNLSHSRWMISGRYDPTNWVGTCGSAPASFTWELL
                     SCIVNGIELLTTPVVVTVPFASMTLTPAGIPTNYATAFNALPELAGNDIVLEPDMTAY
                     SWNTAKPFLLGFRQYTNDVICTPALTSRIDFIKGGVSDGWDYAISPNLIDMQTKTMSI
                     YNDASIIYPHQPVNSCVAI"
BASE COUNT          449 a          206 c          229 g          429 t
ORIGIN      
        1 ataagtcctt ttacagctac aacacctgga atgccgctaa ctaatgttag tctagtacaa
       61 tctatgacta atatttcagc tgccagtatg gggttatctt ctactgattt aatatatgca
      121 atcactaatg ataatcaacc tgtatggttc ttaagtccag cagctatagc gttactccca
      181 aataatactt atttacaacc ttacatgtat tttacagata ctccagggac ttataataag
      241 tgtgatatta atattgctgc tccttatact attgcagatg ttaaacaaac ttcagctcaa
      301 ggaggacaat ctacttgtac accaattcaa gaaataaaag aaaaagatag ttgtacagga
      361 ttagaaactt atagatatat tacagaagat ggtacaggta atcttgttga tataaatact
      421 gtaattccta attttgatga aacaaaagtc ttattaagtt gtcctgaatc tccaattctt
      481 ttagaaaaag aagtatgtgg cactattgat ggttcagttg atacttatga attaattaaa
      541 gtgtatacac gtaatcctac aacaggagta tctactatat tattttatga agataataaa
      601 ggtaatcaaa ttacaggtgc agtagtagaa tcttgttgta catgtaatac attatgtgat
      661 gttcccactg taattgtacc tgttcctaat caaagatgtt taggatatag cagtaacaat
      721 gaccctaatt taagtcattc aaggtggatg atttcaggta gatacgatcc tactaattgg
      781 gttggaactt gtggctctgc accagcatcg tttacatggg aattgctatc ttgtattgtt
      841 aatggaatag aattacttac tacaccagta gtagtaacag taccttttgc atcaatgaca
      901 ttaacacctg cgggcattcc tactaattac gctacagcat ttaatgcatt gcctgaatta
      961 gctggaaatg atatagtgtt agaacctgac atgactgctt atagttggaa tacagctaag
     1021 ccatttttac ttggatttag acaatataca aatgatgtta tttgtactcc tgctttaaca
     1081 agtagaatag actttataaa aggtggtgta agtgatggat gggattatgc aataagtcca
     1141 aatttaatag atatgcagac taaaacaatg tctatttata atgatgcttc aattatttat
     1201 cctcatcaac cagtaaactc atgcgtagca atataatagc taaaacaaga gaagagtggg
     1261 agtacctagc tagtatagga gatcttcaag ggttagaatt tatgaaattg cac
//
LOCUS       DAOSAB010000166         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_69959_length_1311_cov_2.386146, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000166 DAOSAB010000000
VERSION     DAOSAB010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69959_length_1311_cov_2.386146"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PK432_02925"
     CDS             complement(<1..646)
                     /locus_tag="PK432_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59910.1"
                     /translation="MPDQMISTAIDNAVDKLDKGEPINPLTDQLETIIITVLSDLLLG
                     ASNVKPEEVKQTLVEHNVPEPIAKEVADNITSPQAKNLASQLDSSFEKAVNNLVNTIV
                     ETHGDEIMSSAKEKASKIISEVSQNLDLKDKVDSGLIQPAQTLTPIITPPPEITPAPI
                     TPPETVYKPETTPTPISKPIVAEVIEKEVKQPALPEQKGVYEVSSKGDKRFSALY"
     gene            complement(680..1090)
                     /locus_tag="PK432_02930"
     CDS             complement(680..1090)
                     /locus_tag="PK432_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59911.1"
                     /translation="MKEADKFAQQVYVEMGPYAFDKTVQPKGLLSPSGVAEPTSDIYN
                     PESKLNIIPQIAGGIGQVVGQYGVGGALARKTGLMGAGLAEMGAVAGTNLYNMARQNG
                     ASVDEALTLSSLSALQETATELLPFGTYLKSNKS"
BASE COUNT          408 a          235 c          201 g          467 t
ORIGIN      
        1 catataaggc gctaaatctc ttatcacctt tagatgaaac ctcatataca cctttctgtt
       61 caggtaatgc tggttgtttt acttcctttt ctattacttc tgctactata ggtttagaaa
      121 taggtgtagg agtagtttct ggtttataaa cagtttcagg aggagtaata ggagcaggag
      181 taatttcagg aggtggagta ataataggag ttaaggtttg agcaggttga ataagaccac
      241 tatctacctt atcttttaga tctaaattct gagatacttc tgatattatc ttagaagcct
      301 tttctttagc cgatgacatt atctcatcac catgagtttc tacaatagtg ttaactaaat
      361 tatttacagc tttctcaaat gatgaatcta actgagaagc taagttttta gcttgaggag
      421 aagtaatatt atcagctact tcttttgcta taggttcagg aacattatgt tcaactaatg
      481 tttgttttac ttcttcaggt ttaacattgc tagctccaag caataagtca gataaaacag
      541 taattatgat tgtttctaac tgatctgtca gagggttaat tggttcaccc ttatctaact
      601 tatctacagc attatcaata gcagtagaaa tcatttgatc aggcaaatca cttcaagcaa
      661 tagataaaac ttttttcaat caggatttat tgcttttaag ataagtccca aaaggaagaa
      721 gttctgtagc agtttcttgt aaggcactta aactacttaa agttaatgcc tcatctacac
      781 tagcaccatt ctgtctagcc atattataca aatttgttcc agcaactgca cccatttctg
      841 ctaatccagc acccattagt ccagtttttc tagcaagagc accaccaaca ccatattgac
      901 caactacctg tcctatacca ccagctattt gaggaattat atttaattta gattcaggat
      961 tatatatatc actagtaggt tctgctactc cactaggaga taataaacct tttggttgta
     1021 cagttttatc aaaagcataa ggacccattt ctacataaac ttgttgagca aacttatctg
     1081 cttccttcac taattcattt acaatagctc aagttttata actctgttta cgtatatcac
     1141 ttattgtatt agaaggaata ccaacctttt gtaagacatc tgatcataac ttatctttta
     1201 attgagttaa tgttaatgta gcttttctag cattagtgat tgctatatct aaattatttt
     1261 ctaaccagtt agtagataaa gctatgatat ctcctaagaa acctgattta t
//
LOCUS       DAOSAB010000167         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_70096_length_1309_cov_2.885965, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000167 DAOSAB010000000
VERSION     DAOSAB010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70096_length_1309_cov_2.885965"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1228
                     /locus_tag="PK432_02935"
     CDS             104..1228
                     /locus_tag="PK432_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59912.1"
                     /translation="MFNTKIKNILIGTILILLFLITPTYAAKIETGDYTLNKEEIIED
                     DLYVSGGNITISGIVDGDLLAAGENILVDGTVTGDIYSAGNSIKITGNVSGNTFVLAS
                     NATISAILRKNLYITAAIADVKGSIEGDLMAAVGQINIEGVVLEDVRVAANQLKSTAS
                     VGEDFLVSSSNYSIDESSVAGEVINNRKQAISSKFTLEDLSGIGSGLTIINFMGMYLA
                     GIIIILLAPVKTLRIEKKITSSWQELLKSYAVGIAALVVGPIAALILLLTGIGAPLAL
                     LITAALAFLSIFGILYVESAIGHKILKMTGQKDYVRFFSLLIGRCISLIISFIPVLRG
                     IYSISLISVAIGSAVRMKYDSFPKQTQEETKTEVVKKKKK"
BASE COUNT          526 a          184 c          220 g          379 t
ORIGIN      
        1 aaaactctca tcataaattg tagattatac ataaattatg agtatctaac aaaacaaact
       61 ctactatgct atactatttt aatataactt aacagtaata aatatgttta atacaaaaat
      121 aaaaaacata ttaataggaa caatattaat cttattattc ctaataacac caacatatgc
      181 agctaaaata gaaacaggag actataccct aaataaagaa gaaattattg aagacgatct
      241 atatgttagt ggaggtaata taactataag tggaatagta gatggagatt tgcttgctgc
      301 aggagaaaat atactagtag atggaacagt aacaggagat atatatagtg caggaaacag
      361 tataaaaata acaggaaatg tatcaggcaa tacttttgta ttggcatcta atgcaacaat
      421 atctgcaata ttaagaaaga atctgtatat tactgcagca atagcagatg taaaaggaag
      481 tattgaagga gaccttatgg cagcagtagg acaaattaat attgagggag tagtacttga
      541 agatgtaaga gtagcagcaa accagcttaa atcaacagca tctgttggag aagatttcct
      601 tgtatcaagc tcaaactatt caatagatga aagcagtgta gcaggagaag taataaataa
      661 tagaaagcaa gcaatttctt ctaagtttac attagaagat ttatcaggaa ttggttctgg
      721 tcttaccata attaacttta tgggaatgta tttagcagga ataataataa tacttcttgc
      781 acctgttaaa acattaagaa tagaaaagaa aattacatcc tcatggcagg aattacttaa
      841 aagctatgca gtaggaatag cagcattagt tgtaggacca atagcagcat taatacttct
      901 tcttacaggc ataggagcac ctcttgcatt gttaattaca gcagctttag cattcctatc
      961 aatatttggg attctttatg tagaatctgc aataggacat aaaatactta aaatgacagg
     1021 acaaaaagat tatgtaagat tcttctcact gttaattgga agatgtattt ctctaataat
     1081 ctcctttata cctgttctaa gaggtatata ctcaatatct ttaatctctg ttgcaatagg
     1141 ttctgcagta agaatgaaat acgactcatt tcctaaacaa acacaggaag aaacaaaaac
     1201 agaagtagta aagaaaaaga agaagtaatc aagaaggtat atgaataaaa aaggggaagt
     1261 tctcttcccc ttttaattag tcaacaatac tcctacttag ctaacatct
//
LOCUS       DAOSAB010000168         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_71075_length_1297_cov_2.918680, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000168 DAOSAB010000000
VERSION     DAOSAB010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71075_length_1297_cov_2.918680"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="PK432_02940"
     CDS             complement(<1..1162)
                     /locus_tag="PK432_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59913.1"
                     /translation="MRFLKYLFTIILSFFILLILSQSVFAVDIVKRKDIGMDETLSVQ
                     MDIYSLPQKIYISQLEGYNKEFLGKPNEWIKALYYYSVTRLGLNDIPYNYLIDKSGNI
                     YEGARGGEGINPGLEGGQNVVLIGIMDNNTTLAPRTSEALNSLVQELSYKYGIKNTNW
                     EFVELKLKKSSDKVSYMTYSVSAGELRSSIVSSLAEVQWSDSEHLKYKGSIVSVDYAK
                     EVEVGNRLDVKVKIKNENDFIWFGDLSYIYISTVDSKETPHAINMEWESFSKPTHLQN
                     VNVKPGETVEVTFQMEAKSKPGEYKESFYFMKSADNIIEGSTFDVEFTITRGNNKLIQ
                     LTSPEFGYVNIRECRWYSCKVLEVADEGWVYAIIKKEEGWYQIKFGKDQTGWV"
BASE COUNT          393 a          272 c          152 g          480 t
ORIGIN      
        1 atacccaccc tgtttgatct tttccaaact taatctgata ccatccctct tcttttttta
       61 ttattgcata aacccatcct tcatcagcaa cttccaacac tttacagcta taccatctgc
      121 actctctaat atttacatat ccaaattcag gtgatgtgag ctgaattaat ttgttatttc
      181 ctctagtaat agtaaactca acatcaaaag tacttccttc tataatatta tctgcagatt
      241 tcataaagta aaaactctct ttatattccc caggtttact ttttgcttcc atctggaatg
      301 ttacttctac tgtttctcct ggcttaacat taacattttg taaatgagta ggcttactga
      361 agctctccca ctccatattg atagcatgag gagtttcttt agaatccaca gtagaaatat
      421 atatgtaact aagatcgcca aaccaaataa agtcattctc attctttatt tttactttta
      481 catctaatct atttccaact tcaacctctt ttgcataatc tactgataca atactccctt
      541 tgtatttaag atgttcacta tcagaccatt gtacttctgc aagtgatgat acaatagagc
      601 ttctaagctc tcctgctgaa acagaatatg tcatgtacga taccttatca ctgcttttct
      661 ttaatttaag ttcaacaaat tcccagttag tattctttat tccatatttg taagaaagct
      721 cctgtaccaa agagttaaga gcttctgatg tacgtggagc aagagttgta ttattatcca
      781 ttatccctat taaaacaaca ttttgtccac cttctaagcc aggatttatt ccttctcctc
      841 cccttgcccc ttcatatata tttccgcttt tatctattaa atagttgtat gggatatcat
      901 ttaaaccaag ccttgttaca ctgtagtagt aaagagcttt tatccattca ttgggtttac
      961 ctaaaaactc tttgttatat ccttctagct gtgatatata tattttttga ggcaatgaat
     1021 atatatccat ttgaactgat agtgtctcat ccatacctat atctttcctt ttaacaatat
     1081 ctactgcaaa aacagattga gaaagaatta acaatatgaa gaaacttaaa ataattgtaa
     1141 aaagatattt taggaatctc ataacagata ttgtatcata cttttaatat ggtgctgctc
     1201 cttcctcctc caatccttcc tcatacaata catcattttc atcaatcact ctggttataa
     1261 ggtaggaagg gtcaactgca catacaaaca tttttgt
//
LOCUS       DAOSAB010000169         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_71485_length_1292_cov_1.956346, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000169 DAOSAB010000000
VERSION     DAOSAB010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71485_length_1292_cov_1.956346"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PK432_02945"
     CDS             complement(<1..291)
                     /locus_tag="PK432_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59914.1"
                     /translation="MKKKPLSIIIISIVGIFLIVFIFAVLGTSRSQYISSSDDSRSSM
                     WNSMDSMDSVGSLGFSEGYYKGDPSTYQNETANNNQEGSKIQKNGSVSVLVEN"
     gene            complement(370..762)
                     /gene="rpsI"
                     /locus_tag="PK432_02950"
     CDS             complement(370..762)
                     /gene="rpsI"
                     /locus_tag="PK432_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009007558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOK59915.1"
                     /translation="MTKYFEGIGRRKTSTARVRVYSGKKASTVNDKAVDIYFANNKDA
                     DKLLNQPFIITDLADKYYFTAKVKGGGINSQIDAIRLGLGRALCEVDESLKPVLRKAG
                     LLTRDPRAVERKKYHHVKARKKPQFSKR"
     gene            complement(777..1211)
                     /gene="rplM"
                     /locus_tag="PK432_02955"
     CDS             complement(777..1211)
                     /gene="rplM"
                     /locus_tag="PK432_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOK59916.1"
                     /translation="MKYKTQWNKKEDIERNWYIVDVKDQVLGRAATKIASLLIGKGKV
                     NIVPNMDCGDYVVVVNSDFVKVTRGKELKKKYYSYSGYPSGLKVTTFDEQMKKDSRKV
                     IESAVKNMLPKNKMKDGRMSRLFVYKGSEHKHEAQKPVEYKI"
BASE COUNT          389 a          259 c          147 g          497 t
ORIGIN      
        1 attttctact aacacagata cgctcccatt cttctgtatc ttagagccct cctgattatt
       61 attagctgtt tcattttgat atgtggaggg atctccctta tagtaacctt cagaaaatcc
      121 aagacttcca acactatcca tactatccat actattccac atactacttc gactatcatc
      181 tgaagatgag atgtattgtg atcttgatgt tcctaaaaca gcaaaaataa atacaattaa
      241 aaatatcccg actatggaga tgataataat agaaaggggt ttctttttca tataaatgaa
      301 taaaaaagtt atatattaat acaatacata tattaacatt tttattcaag tagaaacaaa
      361 gatattttgt taacgcttgg agaactgagg cttctttctt gctttaacat ggtggtactt
      421 ctttctttca acagccctag gatctcttgt aagcaaacct gcttttctaa gtacaggttt
      481 aagagactca tcaacttcac acaatgctct ccctagtcct aatcttatag catcaatttg
      541 agagtttatc ccacctccct ttactttagc agtaaagtaa tatttatctg ctagatcagt
      601 aataataaaa ggctgattta aaagtttatc tgcatcttta ttattcgcaa aatatatatc
      661 aacagcctta tcattaacag ttgatgcttt cttgccagag taaactctta ctcttgcagt
      721 tgatgttttt cttcttccta ttccttcaaa atacttagtc attattaatc ttaattttaa
      781 attttatatt ccacaggttt ctgtgcctca tgcttatgtt cacttccctt atacacaaaa
      841 agtctagaca ttcttccatc tttcattttg ttcttaggaa gcatattttt tacagcagat
      901 tctattactt ttcttgaatc tttcttcatc tgctcatcaa aagtagttac ttttaatcca
      961 cttggatacc ctgaatagct ataatatttc ttctttaatt ctttacctct tgtaaccttt
     1021 acaaaatcac tattgacaac aactacataa tctccacaat ccatattagg aactatattt
     1081 accttacctt taccaattaa aagagatgct atctttgtag ctgcccttcc aagtacttga
     1141 tcttttacat caacaatata ccaatttctt tctatatctt cttttttatt ccattgtgtt
     1201 ttgtatttca tagtatttta aacaaatatg ttaaagacct gatcttgttc taactctctc
     1261 tttcttaaca ggtgcagtct tttgtactgc ag
//
LOCUS       DAOSAB010000170         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_71833_length_1288_cov_1.918086, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000170 DAOSAB010000000
VERSION     DAOSAB010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71833_length_1288_cov_1.918086"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="PK432_02960"
     CDS             complement(<1..1242)
                     /locus_tag="PK432_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOK59917.1"
                     /translation="MLSKDTKFEIKDESTNTVALVNYNLNITVDNNIPQLAKAYPENY
                     EVEKDKTATTFTFKGQDRIGKPVYLEFGTERDYKFKVVLKTQKTDTIIPEKYSSNFSA
                     LSTNIYEITLPREFSENNQSVRIEKISPTPTKLIIDTEGNVRATFEVPANKESEIIVS
                     GYIHLEQEPLEEAVQIPNIPLKQYFEKIKSDQNLTKYLSATKYWEINDSFIKQEAEKL
                     LEGKQTVLDVIQADYQYINDKLEYNSSKASDPSRIRIGAKAALQGGDSVCMEYSDAMI
                     AILRAQGIAARAASGYTNINPSVTEKESHQWVQVWIPEYGWLTVDPSHESSNMTIGQN
                     VQYVLWNTTHDDSNVDIKAYSANEFKIDTSKYNISISAVDKKEIPLNLLSYSDIKTEN
                     KENSTKDTINMIVKTTSIGKIA"
BASE COUNT          348 a          207 c          200 g          533 t
ORIGIN      
        1 ggcaatcttt cctatagaag ttgtttttac aatcatatta attgtatctt ttgtactatt
       61 ttctttgttt tcagttttta tatcagagta tgagagtaaa ttgagaggaa tttctttttt
      121 atcaacagca gatattgata tattgtactt tgatgtatca atcttaaatt catttgcaga
      181 gtatgccttt atatcaacat tactatcatc atgtgttgtg ttccatagaa catactgtac
      241 attttgtcca attgtcatat tggagctttc atgagaagga tctacagtta gccagccgta
      301 ttctggaatc catacctgta cccattgatg actttctttt tcagttacag aagggtttat
      361 atttgtatac cctgatgctg ctcgagcagc tattccctgg gctcgcagta ttgcaatcat
      421 agcatcagag tactccatac acacactatc tcctccttga agtgcagctt tagcaccaat
      481 tcttattcta ctgggatcag aagcttttga gctattatat tcaagtttgt catttatata
      541 ttgatagtca gcttgtatta catctaaaac agtttgttta ccttctaaaa gtttttctgc
      601 ttcttgttta ataaaagaat catttatctc ccaatatttt gttgcagaaa gatactttgt
      661 taagttttga tcacttttta ttttttcaaa atactgttta agtggaatat taggaatttg
      721 tactgcttct tctaaaggtt cttgttctaa gtgaatatat cctgatacaa taatttcgct
      781 ctctttattt gcaggtactt caaatgtagc tcttacatta ccctctgtgt caataattaa
      841 tttggttggt gtaggagata tcttttcaat tcttacactt tgattgtttt ctgagaattc
      901 ccttggaagt gtaatttcat atatatttgt agatagtgca gaaaagttag aagagtattt
      961 ttctgggatt attgtatctg tcttttgggt tttaagtaca accttaaatt tataatctct
     1021 ctctgttccg aattcaagat atacaggttt tcctatccta tcctgtcctt tgaatgtaaa
     1081 ggttgttgct gttttatcct tttcaacttc ataattttca ggatatgctt ttgctagttg
     1141 aggaatattg ttatcaaccg ttatattaag attgtaatta actaaagcta cggtatttgt
     1201 tgactcatct tttatttcaa attttgtgtc cttgctaagc atagggtatt caattaatac
     1261 ccagttatat acatttttaa catagtcg
//
LOCUS       DAOSAB010000171         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_72283_length_1282_cov_2.365933, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000171 DAOSAB010000000
VERSION     DAOSAB010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72283_length_1282_cov_2.365933"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..>279)
                     /locus_tag="PK432_02965"
     CDS             complement(93..>279)
                     /locus_tag="PK432_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOK59918.1"
                     /translation="LSDFNPKGEISRMYNAYQEEDGLSKRALYVIDKDGVIAWGYISP
                     IGINPGADGILKALEEL"
     assembly_gap    280..289
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<290..553)
                     /locus_tag="PK432_02970"
     CDS             complement(<290..553)
                     /locus_tag="PK432_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOK59919.1"
                     /translation="MKDQPLKKGMKAPDFSLKSTLDQEVSLKDFLGKPVILAFYPADF
                     SPVCGSELTLFNEVLPEFKKYDAQLFGISVDNIWSHIAFAKERN"
     gene            complement(571..1095)
                     /locus_tag="PK432_02975"
     CDS             complement(571..1095)
                     /locus_tag="PK432_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006667601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOK59920.1"
                     /translation="MSKLIIQPNNEDHIQGKEDSSITLVEYGDFQCPYCGEAYPIVKE
                     IQKIKGDVLRFVFRNFPLSEIHSYALHAAYAAEAAGKQHKFWEMHDILFENQENLKDS
                     DLRLYSEKLSLDIDQFTKDMNSDEVAKKVETDIMGGIKSGVNGTPTFFINGIRFDGPS
                     ELNSLLKAIELADK"
BASE COUNT          424 a          226 c          168 g          454 t
ORIGIN      
        1 gttatatggt attacacaga attcaataag acatatacac agtcaaagga gagagggtta
       61 agagatttaa ataaacagta taaagaaact aattataatt cttctaatgc ttttaatatt
      121 ccatctgcac ctggatttat accaatagga gaaatataac cccaagctat aacaccatct
      181 ttatcaatta catacaaagc tcttttagat aacccatctt cctcttgata tgcattatac
      241 attcttgata tctcaccttt aggattaaaa tctgagagan nnnnnnnnna ttcctttctt
      301 ttgcaaatgc tatatggctc catatattat ctacagagat tccaaataac tgagcatcat
      361 atttcttaaa ctcaggtaaa acctcattaa aaagtgtcaa ctcacttcca cacacaggac
      421 taaaatctgc aggatagaaa gctaaaataa caggtttgcc taagaaatct tttaaagaca
      481 cttcctgatc aagagtagat tttaaactaa aatcaggagc cttcattcct tttttaagag
      541 gttggtcttt catttaaagt atttaaaatt ttatttatct gctaactcaa ttgctttcag
      601 cagagaattt aactcagatg gaccatcaaa acggatacca tttataaaga atgtaggagt
      661 accattaact ccacttttaa taccacccat aatatctgtt tctactttct tagcaacttc
      721 atcagagttc atatctttag taaattgatc aatatctaga gatagttttt cagaatataa
      781 tcttagatcg ctgtctttta gattctcttg attctcaaaa agaatatcat gcatctccca
      841 gaacttatgt tgtttaccag ctgcttcagc agcatatgct gcatgtagtg catatgaatg
      901 tatttcagat aatgggaaat tacgaaacac aaatcttagt acatctcctt ttattttttg
      961 aatttcttta acaataggat atgcttctcc acagtatggg cactggaaat ctccatattc
     1021 aacaagagtt atactgctat cctcttttcc ttgtatatga tcttcattgt ttggttgtat
     1081 tattaatttg ctcataggta cgattatatg gtttgtagat taattcatta tattacaaac
     1141 tatataaata tctagtattc actactaaac cagtttctca ttagactttg cttctactat
     1201 cttcttgaga tttttttctt agagcctaag gttacagtat aaaaagggaa gataagatat
     1261 ctctttcttg atataattcc tt
//
LOCUS       DAOSAB010000172         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_72900_length_1274_cov_3.456932, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000172 DAOSAB010000000
VERSION     DAOSAB010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72900_length_1274_cov_3.456932"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PK432_02980"
     CDS             complement(<1..515)
                     /locus_tag="PK432_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOK59921.1"
                     /translation="MEDIIKKELDTLLSLVDIDAQYDLNVEESNGVKYIKISFNGENL
                     GYLIGNHGKHLESLQYVFSLILRKKFEEGVEYRVLMDVGGYKEERNKKIERMALQKAD
                     DARILGEPIELEPMSPSDRRVVHMALQVFDDIKTESVGEGNDRRVKIIPISDKEILKR
                     KEDSDSEEESE"
     gene            complement(519..>1274)
                     /locus_tag="PK432_02985"
     CDS             complement(519..>1274)
                     /locus_tag="PK432_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOK59922.1"
                     /translation="VIARLLMIPLVKKQTKMTKQMAMLKPELDKLQKKYGNDKEKLAQ
                     EQVKLYRRIGYNPLGCLVTFIPQLIIPLVLIGVIQSVTSSSLEGLYPWVVDITGIKQG
                     ASINTQFLFWDMTKSFSDVSGEFGRLSSQALPYILLSLMVGVTQYFTTLFTQKINEIG
                     KPKKKKVEKKKEKTQEETIASMQESMQKSTMLMFPLMTVIFSISMPAVFGWYWMLQSL
                     LLIIQYISLDFDKTKKGVQNLLDVLKKDKFKKQ"
BASE COUNT          396 a          279 c          138 g          461 t
ORIGIN      
        1 tcctcactct cctcttctga atcactatct tctttccttt ttaatatctc tttatcacta
       61 ataggaataa tctttaccct tctatcatta ccctctccta cactctctgt cttaatatca
      121 tcaaatacct gtaatgccat atgtaccact cttctatctg aaggactcat aggttctagc
      181 tctattggtt ctcctaatat ccttgcatca tctgcttttt gcaatgccat tctctctatc
      241 tttttattcc tttcctcttt atacccccca acatccatta atactctata ctccactccc
      301 tcttcaaact tctttcttag gattaagcta aaaacatact gaagagattc taaatgctta
      361 ccatgattac ctataagata acctaagttc tctccattaa aagaaatctt tatatactta
      421 acaccattac tctcctctac atttaaatca tactgtgcat ctatatctac taaagagagt
      481 agagtatcaa gctctttctt tattatatct tccatatatt attgtttttt aaatttatct
      541 tttttaagaa catctaaaag gttttgaaca cctttctttg ttttatcaaa atctaatgaa
      601 atatattgaa taattagaag aagagactgc aacatccaat accatccaaa tacagcaggc
      661 atactaatgg aaaatataac tgtcattaaa gggaacataa gcatagtaga tttctgcata
      721 gattcctgca tagaagcaat tgtttcttcc tgtgtcttct cttttttctt ttctactttc
      781 ttcttctttg gcttaccaat ttcatttatt ttttgagtaa ataaagtagt gaaatactga
      841 gtaacaccaa ccattaaaga aagaagaata tatggaagag cctgagaaga aagtcttcca
      901 aactctcctg aaacatcact aaatgattta gtcatatccc aaaacaaaaa ctgtgtatta
      961 atactagcac cctgttttat cccagttata tctacaaccc atggataaag accttcaaga
     1021 ctactgcttg taacactttg tataacaccg ataagaacta aaggaataat taattgtgga
     1081 ataaatgtaa caagacaccc aagaggattg tatcctatcc tcctgtataa tttaacctgc
     1141 tcctgtgcca atttctcttt atcattccca tacttcttct gcagcttatc aagttcaggt
     1201 ttaagcattg ccatctgctt agtcattttt gtctgttttt taaccaaagg aatcattaac
     1261 agccttgcta taac
//
LOCUS       DAOSAB010000173         1271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_73178_length_1271_cov_2.305921, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000173 DAOSAB010000000
VERSION     DAOSAB010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73178_length_1271_cov_2.305921"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PK432_02990"
     CDS             <1..1206
                     /locus_tag="PK432_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOK59923.1"
                     /translation="KEVELPISIILSRMERRGFALDVDKLLKLKKEVEDEIRKIEKEI
                     YSIVGHEFNISSPKQLSDVLFKELGLPNKSKGSTRESVLDDMLGTHPIVEQLLYYRDA
                     TKMLSTYIDPLYSNSIPEEEGIRSIHTDFKQTGTTSGRFSSVNPNLQNIPLEGAWANS
                     LRECFVARKGFKLLGMDYAQMELRIMADISDDKLLKKDFINGLDIHSATAARVLNKKM
                     SEITHKERSLGKVVNFGMIFGQTAFGLASLLGVDRETASKYISSYFEHYKGVEEYMRK
                     IEYEAFKKGYVQTMFGTTRHIAGIRSKNVRLRAASAREAINMPIQGSEADIMKFVMKK
                     LYELIEEKYSKEAYILLQIHDEIIMEVKESVVEKFSKEAKRIMLESVNLDVPLDVHVS
                     VGNCMAELK"
BASE COUNT          471 a          152 c          273 g          375 t
ORIGIN      
        1 aaggaagtag agcttcccat atccattatt ctttcaagaa tggaaagaag aggttttgct
       61 ttagatgttg ataaactctt gaaattaaaa aaagaggttg aagatgagat aagaaagata
      121 gaaaaggaaa tatacagtat agtaggacat gagtttaata ttagttctcc taagcagctt
      181 tctgatgttt tgtttaagga attaggtttg cctaataaat ctaaaggaag cacaagagaa
      241 tcagtgctag atgatatgct tggtacacac cctattgttg agcagctttt atactaccga
      301 gatgcgacta aaatgttaag cacatatata gatccactgt acagtaatag tatccctgaa
      361 gaagaaggaa ttcgctcgat acatactgat tttaaacaaa caggtacaac atcagggaga
      421 ttctcttctg taaatcctaa cttgcagaat attccactgg aaggagcatg ggctaatagt
      481 ttaagagagt gctttgttgc aagaaaaggt tttaagcttt taggaatgga ttatgcacaa
      541 atggagctta gaattatggc agatatatct gatgataaac ttcttaaaaa agactttata
      601 aacggtcttg atattcatag tgctactgct gcaagagtat taaataaaaa gatgtcagag
      661 attactcata aggagagaag tttaggaaag gttgtaaatt ttggaatgat atttggacaa
      721 acagcatttg gcttagcatc acttcttggt gttgatagag agactgcttc aaagtatatc
      781 tcttcatatt ttgagcatta caaaggagta gaggagtata tgaggaagat agagtatgaa
      841 gcatttaaga aagggtatgt tcaaacaatg tttggtacta caaggcatat tgctggtata
      901 aggtcaaaga atgtaagact tagagctgca tctgcaagag aggctataaa tatgccaata
      961 cagggtagtg aagctgatat tatgaagttt gtaatgaaaa agctttatga attaatagaa
     1021 gagaagtatt caaaagaggc atatatttta cttcagatac atgatgaaat aattatggaa
     1081 gtaaaagaat ctgttgttga gaagttttca aaagaagcta aaagaataat gcttgaaagt
     1141 gtaaatttag atgttccttt agatgtccat gtctcagtag gaaactgcat ggcagagtta
     1201 aaataattag tataaatata tggcagaaaa gaaaataagg tatcaagatt tatcctcagc
     1261 aggcattgac g
//
LOCUS       DAOSAB010000174         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_73775_length_1264_cov_4.226634, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000174 DAOSAB010000000
VERSION     DAOSAB010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73775_length_1264_cov_4.226634"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PK432_02995"
     CDS             complement(<1..330)
                     /locus_tag="PK432_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK59924.1"
                     /translation="MLISAFQKSTLLDYPRKLACLVFTYSCNLRCEYCHNPELVIQPF
                     RKDISVSEDEIFSFLQKRKSLIDGIVITGGEPTLQKDLIPFIRKIKDMGFLVKLDTNG
                     TNSKVVKK"
     gene            complement(331..471)
                     /gene="nrdD"
                     /locus_tag="PK432_03000"
     CDS             complement(331..471)
                     /gene="nrdD"
                     /locus_tag="PK432_03000"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOK59925.1"
                     /translation="MTKKEKKVVIRQRCEVYSRVVGYIRPVTQWNPGKQSEFGDRVTF
                     HI"
     gene            complement(490..>1264)
                     /gene="nrdD"
                     /locus_tag="PK432_03005"
     CDS             complement(490..>1264)
                     /gene="nrdD"
                     /locus_tag="PK432_03005"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOK59926.1"
                     /translation="KKDFFKRLDKLMDLAKQSLLIKRFQIEKYMDQGLYPYSKFYLKD
                     IKERFGEYFKNHFNTIGILGLNEALINFLGEGNDITSAKGRKFGLEIMDHMRERLMDY
                     QLQTNQLFNLEATPGESTTYRFAKADKKKYGDKIIAASDLGKKMNESPYYTNSSQLPV
                     GFTDDIFNALDLQDEFQCKYTGGTVLHIFLGERMPSTQSVKTLVRKVAENYKLPYFSI
                     TPTFSICPKHGYIAGEHYYCPKCDAEAGYVDGMQFNEVI"
BASE COUNT          406 a          230 c          165 g          463 t
ORIGIN      
        1 ttttttgaca acttttgagt ttgtaccatt tgtatcaagc ttaactaaaa atcccatatc
       61 tttgattttc ctaataaagg gaattaaatc tttttgtaaa gttggttcac cacctgtaat
      121 aacgatacca tctattaaag attttctttt ttgtaaaaaa gaaaatatct catcttcact
      181 taccgatata tcctttctaa acggttgtat tacaagttct ggattatgac agtactcaca
      241 ccttaagttg cagctatatg taaacacaag acaagcaagt ttcctaggat aatcaagaag
      301 cgttgatttt tgaaaggcag agattaacat ttaaatatga aatgttactc tatcgccaaa
      361 ttcagattgt ttaccaggat tccactgagt aacaggtcta atatatccaa caactcttga
      421 atacacctca catctttgtc ttataacaac ctttttctct tttttagtca tatgtataat
      481 aataaaaatt taaattactt cattaaactg cattccatct acatatccag cttctgcatc
      541 acatttagga cagtaatagt gttcaccagc aatatatcca tgtttagggc atatactaaa
      601 tgtaggagta attgagaaat atggaagttt gtaattctcg gctacctttc ttacaagagt
      661 ctttacactc tgtgtagagg gcattctttc tcctaagaat atatgtagta cagtccctcc
      721 agtatattta cactggaact catcttgtaa atcaagagca ttaaatatat catctgtaaa
      781 acctacaggg agctgagatg agtttgtata atagggggat tcgttcatct ttttacctaa
      841 atccgatgct gcaattattt tatccccata tttcttttta tctgcttttg caaatctgta
      901 agtagtactt tcaccaggag tagcttctag attaaacaac tgatttgtct gaagttggta
      961 atccattaat ctttctctca tatgatccat tatttctaaa ccaaactttc ttccttttgc
     1021 agatgtaata tcatttcctt caccaaggaa gttaattaaa gcctcattta aaccaagaat
     1081 cccaattgta ttaaaatgat tcttaaaata ttcaccaaac ctctctttaa tatcttttaa
     1141 gtaaaacttt gagtatgggt ataatccttg atccatatat ttttcaattt gaaacctttt
     1201 aattaacaaa ctctgcttag caaggtccat gagtttatct aatctcttaa aaaagtcttt
     1261 ttta
//
LOCUS       DAOSAB010000175         1253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_74692_length_1253_cov_2.747078, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000175 DAOSAB010000000
VERSION     DAOSAB010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74692_length_1253_cov_2.747078"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1253
                     /locus_tag="PK432_03010"
     CDS             <1..>1253
                     /locus_tag="PK432_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59927.1"
                     /translation="NLRLNIKNENDNDVKQQLLLELQATAKIAHSYFKPYPGREMRHN
                     LLNTMEYYNIDQVVDTEASKNTTIYGLDVQLESLNQDGYYDAYLHTQEVKNESKYLQV
                     ETSKQHDDVSSAVQLKMLSIADLLNIDEILKNETLTTDEINGLKELNTLMYKYQNALK
                     ESAQSRLDYFRNVMRNGDHIDMGELYKIIGSSLEKQGAGENIIKFFKVDPVTNKPVFN
                     SNMPIIRSMLIYYVFSMYSKNITDERTTGKKYIHESAWGNKVVHDTFTGEVIRTDTIK
                     NNPSVYFKEDGTLLDRYITRDLATYEETINGVKHITVEVMISMPPWLNEKEKQFYINH
                     LTKGVGTRIPTEDKRSMVSFKVVDFVDGSKQNAFIMPYFAHMLAGSDFDVDSLYVQHL
                     AYYRDIFGNYRVYGEYTEENKNKEK"
BASE COUNT          522 a          135 c          194 g          402 t
ORIGIN      
        1 aatcttagac ttaatataaa aaatgaaaat gataatgatg taaaacaaca attattatta
       61 gaattacaag ctacagcaaa gatagctcat tcttatttta aaccttatcc tggtagagaa
      121 atgagacata atttgcttaa tactatggag tattataata tagatcaagt agtagataca
      181 gaagcttcta aaaatacaac catatatgga ttagatgtac aattagaaag tcttaatcaa
      241 gatggatatt atgacgctta tttacacaca caagaggtta agaatgaatc taaatatcta
      301 caggtagaaa catcaaaaca acatgatgat gtatcttctg ctgtacaatt aaaaatgcta
      361 tcaattgcag atttattaaa tattgacgaa atattaaaga atgaaacttt aactacagat
      421 gaaataaatg gattaaaaga attaaatact ttaatgtata aatatcaaaa tgctttaaaa
      481 gaatcagcac aatcaagatt agattatttc cgaaatgtaa tgagaaatgg tgatcatatc
      541 gacatgggag aattatataa aattattggt agtagtttgg aaaaacaagg tgctggtgaa
      601 aatataatta aattttttaa agtagatcct gttacaaata aacctgtatt taatagtaat
      661 atgcctatta ttagaagtat gcttatttat tatgtttttt ctatgtatag taaaaatata
      721 acagatgaaa gaactacagg taaaaaatat attcatgaat ctgcttgggg aaataaagta
      781 gtacatgata ctttcacagg agaagttata agaacagata caataaaaaa taatccttct
      841 gtttatttta aagaagatgg gacattactt gatagatata taaccagaga cctagctact
      901 tatgaagaaa caataaatgg tgtaaaacat attacagtag aagtaatgat ttctatgcct
      961 ccttggttaa atgaaaaaga aaaacaattt tatatcaatc atttaacaaa aggtgttggt
     1021 actcgtattc ctacagaaga taaaagatct atggtatcat ttaaagtagt agattttgta
     1081 gatggctcaa aacaaaatgc ttttataatg ccttattttg cacatatgtt agcaggttct
     1141 gactttgatg ttgactcttt atatgtacaa catcttgctt attatagaga tatatttggt
     1201 aattatagag tgtatggaga atatacagaa gaaaataaaa ataaagaaaa gtt
//
LOCUS       DAOSAB010000176         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_75176_length_1247_cov_4.231544, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000176 DAOSAB010000000
VERSION     DAOSAB010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75176_length_1247_cov_4.231544"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1247
                     /locus_tag="PK432_03015"
     CDS             <1..>1247
                     /locus_tag="PK432_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59928.1"
                     /translation="LACASLYIFYLILKKYFKKESIALGGVFLLLTSFQFFLHIPTYM
                     REIPVILSLLLFIYLILNKKSYWLAGLSLLLVFDAKEYVAVMILPGIFFYILWKEKGT
                     TFWESVKRYISSFIKLFLPTLVLLLLMIFTRVVPVNMYALSLVPGVTKGGIQYQLKHF
                     DADVSTINRIEEEAPSIQEDIDNEQSLLSKAWGIFKSYFGKLLYPRTFSFISVPKVIV
                     FPSIFTSIFLLKKKIKKKDFFYVFLFCIFVSFLVVFFLRASFDRYILPILPVIFFYYI
                     FFLKDLVKEKKKYIFTISITALLTAGGMFFEVDYVGIKIFLNVLIIGMYIVYLLYYKK
                     IKNLIYYVITFVGCITFGVAAFFFVANGQIHYYMLWGKDFEIKKVVSYFDENERVLLN
                     DVGWNMLPMVYRGDNQWSAEWKL"
BASE COUNT          360 a          169 c          207 g          511 t
ORIGIN      
        1 acttgcatgt gcctcccttt atatctttta cttaattctt aaaaagtatt ttaagaaaga
       61 atctattgcc ttgggaggag tatttctgct tcttacaagt tttcagttct tcttgcatat
      121 accaacatat atgagagaaa ttcctgttat attatctctc cttttattta tatatctcat
      181 acttaacaaa aagtcatact ggttagcagg tttgtctctt cttcttgtct ttgatgcaaa
      241 agagtatgta gctgtaatga ttcttccagg tatattcttt tatattcttt ggaaagagaa
      301 aggtactaca ttttgggaat ctgttaaaag atatatatct tcatttatta aattgttttt
      361 acccaccctt gtacttcttc tccttatgat ttttacaaga gtagtacctg ttaatatgta
      421 tgcactctct cttgtacctg gtgttacaaa aggtggtata cagtatcagc ttaagcattt
      481 tgatgcagat gtatcaacta ttaaccgtat agaagaagag gctccctcta ttcaagagga
      541 tattgataat gagcaatctt tactgtcaaa ggcatggggt atatttaaaa gctactttgg
      601 taagcttttg tatcccagaa ctttttcttt tatctctgtt cctaaggtaa ttgtatttcc
      661 ttcaatattt acaagtattt ttcttttaaa gaagaagatt aagaagaaag atttttttta
      721 tgtgtttctt ttctgtatat ttgtaagttt tcttgttgta ttctttctta gagcttcctt
      781 tgatagatat attctcccta tacttccagt catctttttc tactatatct tcttcttaaa
      841 agatttagta aaagagaaga aaaaatatat atttacaata tctattactg cactactaac
      901 tgctggtgga atgttttttg aagttgatta tgttggtatt aagatatttc ttaatgtatt
      961 aataatcggt atgtatattg tatatctttt gtattacaaa aagattaaga atttaattta
     1021 ctatgttatt acttttgtcg ggtgtattac ttttggggta gcagcatttt tctttgttgc
     1081 taatggtcag attcattact atatgctttg gggaaaggat tttgaaatta aaaaggtagt
     1141 atcttacttt gatgaaaatg aaagagttct tcttaatgat gttgggtgga atatgctacc
     1201 tatggtatac agaggagata atcagtggag tgcagagtgg aagttag
//
LOCUS       DAOSAB010000177         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_75284_length_1246_cov_2.308984, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000177 DAOSAB010000000
VERSION     DAOSAB010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75284_length_1246_cov_2.308984"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PK432_03020"
     CDS             complement(<1..644)
                     /locus_tag="PK432_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59929.1"
                     /translation="MAEETLPLDPLGTKSLMEADLSLSDVTPYIKDLPAKPLPIESIS
                     IPGNPITGPNARHGVDVDPDMAFAKGYQPKANPYLALKPYTYSADYDNANFERYYSTG
                     DLYNKLGFSPYRDNDSLYNKNMTLGDEFVRAAKQWDNLVGAGFMSGIKSWKTMFTDPL
                     APDMDAARDMARATAIGSSTKDGVGSFFVNTFLNSGYTIGMGADFLAEGLVITGA"
     gene            complement(649..>1246)
                     /locus_tag="PK432_03025"
     CDS             complement(649..>1246)
                     /locus_tag="PK432_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59930.1"
                     /translation="DQEVVLSGLVRQFMTEVSKGWKPTDAKRPVYGAEVTPVGGGQLD
                     QAAITLNLPADVIQDNSGKGKLLGETLGSKIAANRITVFFDKSKVQSPIVERAAYTPF
                     QKAVVNNSYVNVDAFSDYAGDITASFKNGMVTFTTTFKDVDERTGKIYVKGKESVTHG
                     INSADDYYNAMITRLNARNINNQIKIQKLAEAQARLKQ"
BASE COUNT          391 a          242 c          189 g          424 t
ORIGIN      
        1 gcacctgtga taactaaacc ttcagctaag aaatcagcac ccatacctat agtgtatcct
       61 gaattaagga atgtgtttac aaaaaaagat cctacaccat ctttagtaga tgaaccaata
      121 gctgtagctc ttgccatatc tctagctgca tccatatcag gagctaaagg atcagtaaac
      181 attgttttcc aagacttgat accagacata aagccggcac ctacaagatt atcccattgc
      241 ttagctgctc ttacaaactc atcaccaagg gtcatgttct tattatacag agaatcgtta
      301 tctctatatg gagaaaagcc tagcttatta taaagatctc ctgtagaata gtatctttca
      361 aagttagcat tgtcataatc tgcactataa gtatatggtt ttaatgctaa gtaaggatta
      421 gctttaggtt gataaccttt agcaaaggcc atatcaggat caacatctac accatgccta
      481 gcattaggac ctgtgatagg attacctgga atactaatac tctctatagg aagcggtttt
      541 gctggaagat cttttatata aggagtaaca tctgaaagag acagatcagc ctccattaaa
      601 ctttttgtac ctaaaggatc taatggtaat gtttcttctg ccatcttatt attgttttaa
      661 tcttgcttgt gcttctgcaa gcttttgaat tttaatttga ttattaatat ttctagcatt
      721 aagtcttgtt atcattgcat tataataatc atcagctgaa ttaattccat gagttacaga
      781 ttcttttcct tttacatata ttttaccagt tctctcatct acatctttaa aagtagttgt
      841 aaaagtaacc ataccgtttt taaaagatgc tgtaatatct ccagcataat cagaaaatgc
      901 atcaacattt acatagctat tatttacaac agctttttga aaaggagtat aagcagctct
      961 ttctacaatc ggactttgta cttttgattt atcaaagaat acagtgattc tattagcagc
     1021 aattttacta cctaaagttt caccaagaag tttaccttta cctgagttat cttgtataac
     1081 atctgcagga aggtttagag taatagcagc ttgatcaagt tgtcctccac caacaggtgt
     1141 tacttcagca ccatatacag gtcttttagc atccgtaggt ttccaaccct tacttacttc
     1201 tgtcataaat tgtctaacca atcctgatag aactacttct tgatct
//
LOCUS       DAOSAB010000178         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_75722_length_1241_cov_2.965430, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000178 DAOSAB010000000
VERSION     DAOSAB010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75722_length_1241_cov_2.965430"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PK432_03030"
     CDS             <1..280
                     /locus_tag="PK432_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59931.1"
                     /translation="NYNNGGRILPLDSNLLSITGVVCNRYLNDIIPFQKIHIKANGTK
                     IYLSNIQTGVLNEGDTLLLKKGSEIKIGFLNKNEFLKRRIDLSHRFRK"
     gene            complement(281..>1241)
                     /locus_tag="PK432_03035"
     CDS             complement(281..>1241)
                     /locus_tag="PK432_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOK59932.1"
                     /translation="FLLIIGSEWVMKGAVALARRLNVSELVIASTIIALGTGLPTIAV
                     NLFLVLGNSNGVDVAIGNALGTNFVNIGLGLGIPALLLTIVTKYEVFEKEIPIYLGVC
                     SLLTCFMLDSKITRVEGLILLFAYIFALFIIYQYSIRERLKDADKEEIDVDTSTISTI
                     TTEGLTILKSLLYIICGFLLLTLSSLCLNKMTGRLVLDFNISEYVLGLTIIGIGTSIP
                     MIYTSIRSAKKGYTDIIVGNVFGSTIANIALGAGLPAVITPIALNQEALNDIYYFGIL
                     NMIVIFGLLIEMKLLGKNKTLNWVSGLVIIVFYVVYLGSKFIH"
BASE COUNT          482 a          209 c          157 g          393 t
ORIGIN      
        1 caattataat aatgggggca gaattcttcc tcttgattca aatcttctat caataactgg
       61 tgttgtatgc aatagatatt taaatgatat tattcctttt caaaaaatcc atattaaagc
      121 aaatgggaca aaaatatatc tttctaacat acagacagga gtattaaatg agggtgatac
      181 tctacttctt aaaaagggta gtgagataaa aataggattt ctaaataaaa atgaattctt
      241 aaaaagaaga attgatttat cacatagatt tagaaaatag ctaatgaata aacttagagc
      301 caagataaac tacatagaat acaataatta ctaatcctga tacccagtta agtgttttat
      361 tcttcccaag tagtttcatt tcaatcaata aaccaaatat tactatcata tttaaaatcc
      421 caaaataata aatatcattt aatgcctctt gattaagagc aattggagtt ataacagctg
      481 gaagtcctgc accaagtgca atatttgcaa ttgtacttcc aaacacattt cctacaataa
      541 tgtctgtata tcctttcttt gcagatctaa ttgatgtata aatcatagga attgatgttc
      601 caataccaat aattgtaagt ccaaggacat attcagaaat attaaaatct aaaactaatc
      661 tccctgtcat tttatttagg cataatgaag aaagagttaa aagcaaaaaa ccacatataa
      721 tgtataacaa agattttaaa atagttaatc cttctgtagt aattgttgat atagtacttg
      781 tatcaacatc aatctcttcc ttatctgcat cttttaatct ctccctaatg gaatattgat
      841 aaataataaa cagagcaaat atatatgcaa aaagtaatat taatccttct actctagtta
      901 tctttgaatc aagcataaag caggtaagaa gagaacatac tcctaaatat ataggtattt
      961 ctttttcaaa aacctcatat tttgtaacaa tggtaagaag aagtgcaggg atacctaaac
     1021 ctagccctat atttacaaaa tttgtaccaa gtgcattacc gatagcaaca tccaccccat
     1081 ttgaatttcc taaaactaaa aacaaattaa ctgcaatagt gggaagacct gtacccaatg
     1141 ctattatagt tgaggcaata acaagctcac ttacattaag ccttcttgct aatgctacag
     1201 ctcctttcat tacccactct gacccaataa tcagaagaaa a
//
LOCUS       DAOSAB010000179         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_82615_length_1168_cov_2.975741, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000179 DAOSAB010000000
VERSION     DAOSAB010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82615_length_1168_cov_2.975741"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1168
                     /locus_tag="PK432_03040"
     CDS             <1..>1168
                     /locus_tag="PK432_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59933.1"
                     /translation="GETFTVNSKWGGSWSLGWISPNSDRTCGSRFCGPPQGGICTPFV
                     PANVSADISWAESFGNIIISQQCWGDSMEWIGDYDFNDYLLIVTVKPDASCGDGIVGN
                     TTGEECDPPGSLCTLDVGNGSCTNSCKCCVECSVSCPNPLVSSAPVNPPHPHSVSEYF
                     FDNITSCEDRSTCENPQTRYADCYEILSDQPTVSLNIHPESIPTTLSFTSDTHTGSGT
                     YKESGTISDDTVNDFMPYSSIRYQNEGDLFYFEATFQDTDNPIESAYIWFSKSAIKPV
                     TPKFIDLDNSVTPNKYGAKNNSEFGFLLYHDLNTDSWIPYIPAISGSGDSVTDVWKQA
                     KSYSDVEIIDGKTIFSVPASSGQKTAKVLLYSITPANSGKKIVVKFSISFKSSGE"
BASE COUNT          367 a          180 c          239 g          382 t
ORIGIN      
        1 tggagaaaca tttacggtaa actctaaatg gggtggatct tggtctttgg ggtggatttc
       61 tcctaatagt gataggactt gtggtagtcg tttttgtggt cctcctcagg gaggaatatg
      121 tacacctttt gtacctgcaa atgtttctgc agatatatct tgggctgaaa gctttggtaa
      181 tattataata agtcaacagt gttggggaga cagtatggag tggataggag attatgactt
      241 taatgattat cttttaattg ttactgttaa accagatgct tcctgtggag atggtatagt
      301 ggggaataca acaggagaag agtgtgatcc accagggagt ctttgtacat tggatgtagg
      361 aaatggaagt tgtactaatt cttgtaaatg ttgtgtggaa tgctctgtct catgtcctaa
      421 tcctttggta tcatcagcac ctgttaatcc tccacaccca catagtgtat ctgagtattt
      481 ctttgataat attacctcat gtgaagatag aagtacttgt gagaatcctc aaacaagata
      541 tgcagattgt tatgagattt taagtgatca gccaacagtt agtttaaata tacatccaga
      601 atcaattcct actacactct cctttacctc agatacacat acaggatcag gaacatataa
      661 agaaagcgga actatttcag atgatactgt aaatgatttt atgccctact ctagcattag
      721 ataccaaaat gaaggagatt tgttttactt tgaagcaaca tttcaagata ctgataatcc
      781 tattgaatct gcatatattt ggtttagtaa aagtgctatt aaacctgtta cacctaagtt
      841 tatagactta gataatagtg ttactcctaa taaatatgga gcaaaaaaca attcagaatt
      901 tggttttcta ctgtatcacg accttaatac agatagctgg attccatata ttccagcaat
      961 ttcaggttct ggcgatagtg taactgatgt gtggaagcag gctaaatcat atagtgatgt
     1021 agaaattatt gatggaaaaa ctatattctc tgtaccagct tctagtggtc aaaaaactgc
     1081 aaaagttctt ctgtattcca tcactcctgc taatagtggg aaaaaaatag tagtgaaatt
     1141 tagtattagc tttaagagca gtggagaa
//
LOCUS       DAOSAB010000180         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_82727_length_1167_cov_2.778777, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000180 DAOSAB010000000
VERSION     DAOSAB010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82727_length_1167_cov_2.778777"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1167
                     /locus_tag="PK432_03045"
     CDS             <1..>1167
                     /locus_tag="PK432_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59934.1"
                     /translation="RANNQSTFEVGWKALTNVAVIAAKGVIEIPGYLGGMAVGAVGGT
                     ALGINNYYQEATGGKEGRSGWEFASTTAFNNFYLQSTEALKNSIDENVLKIYVPPSVQ
                     EGGLWDKVSSFNYMASIGSEMAGFVLSMYAPGAIVGKLGWGADLIKGLNAAEEVGATL
                     TAGAKVRNAAAKAFVKGSDFIFGEDDIIKAVAGNSKLGRVTGEGLGLATNADDAARVA
                     ASTQATATEIANTLGEATKAIKWTNSAARATDTGLAITFNTALESASEATQSYRDVYK
                     AALAKGYTEDDARRAAATSAIGVFSDNMLILAVTNILGEKFILDGFARAAKGADNNKV
                     AKTLMQDILGSTETNIDKLLKENWFNTSTLGKTLVGRGADKVGRLAGKFALGGLK"
BASE COUNT          402 a          158 c          284 g          323 t
ORIGIN      
        1 agagctaata atcagagtac ttttgaagta ggttggaaag cacttactaa tgttgcagta
       61 attgcagcta aaggtgtaat agagattcct ggttatttag gcggtatggc tgtaggcgca
      121 gtaggtggta cagcattagg tataaataat tattatcaag aagctactgg aggtaaggaa
      181 ggtagaagtg gttgggaatt tgctagtaca acagcattta acaactttta tctacaaagc
      241 acagaagctt taaaaaattc aatagatgaa aatgtattaa agatatacgt accaccaagt
      301 gttcaagaag gtggcttgtg ggataaagtt tcaagtttta actacatggc tagtataggt
      361 agtgagatgg caggctttgt tctaagtatg tatgctccag gagcaatagt agggaaactt
      421 ggttggggtg cagatcttat aaaaggtttg aatgcagcag aagaagtagg tgctactttg
      481 actgcaggtg ctaaagttag aaatgctgca gctaaagctt ttgtgaaagg ctctgatttt
      541 atatttggtg aagatgatat tattaaagct gtagcaggta attctaaatt aggtagagtt
      601 acaggtgaag gtttaggctt agcaactaac gcagatgatg cagcaagagt ggctgctagc
      661 acacaagcta cagctactga aatagcaaac acattaggag aagctactaa agctatcaaa
      721 tggactaact ctgctgctag agctactgat acaggcttag caattacctt taatactgca
      781 ctagaaagcg ctagtgaagc tacacagtca tatagagacg tgtacaaagc tgcattagct
      841 aaaggataca cagaagatga tgcacgtaga gcagcagcaa ctagcgctat aggagtgttt
      901 agtgataata tgttaatttt agctgttaca aatatactag gtgagaaatt catactggat
      961 ggatttgcac gtgcggctaa aggagcagat aataacaaag ttgccaaaac tttaatgcag
     1021 gatattctag gtagtacaga aactaatatt gataaactat taaaagaaaa ttggtttaat
     1081 actagcacat taggtaaaac cttagttggt agaggagcag ataaagttgg tagactagct
     1141 ggcaaatttg ctttaggtgg attgaag
//
LOCUS       DAOSAB010000181         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_83372_length_1161_cov_2.225136, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000181 DAOSAB010000000
VERSION     DAOSAB010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83372_length_1161_cov_2.225136"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PK432_03050"
     CDS             complement(<1..689)
                     /locus_tag="PK432_03050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOK59935.1"
                     /translation="MKKTDISKIALIFIFLVFLLVCIFLAFNIKMGVSPDSYYHLRVS
                     QAYSTTLGIPQNTPQTYQWRDITRIPYLYFWINGRILNINNGVINEVVLLRIINVIYS
                     LGTVYVMYLLSKEIFKDKWKRVLPVFFLTHTLMFVFLSSSINYDNLANLLSILSIYFF
                     VKFVKKNINLKYLLLMLLSLCIGALTKSTVLPVALILVILSAVEIAKRKDKLKKIKAG
                     KEHLLLIPIAI"
     gene            802..>1161
                     /locus_tag="PK432_03055"
     CDS             802..>1161
                     /locus_tag="PK432_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59936.1"
                     /translation="MFLSDVTIKEYIQKGKIEVKGEYEMETNGISLHLGNELLIQKPY
                     QVADSQKPQEIEYIKYNLKERPYVLKPNDFVLGATKELIKTDKDIITMIDGRSTYARL
                     GMTIHLSAMILDGVPFNS"
BASE COUNT          462 a          147 c          179 g          373 t
ORIGIN      
        1 aaaattgcta ttggtattaa cagcagatgc tcttttccag cttttatctt ttttagcttg
       61 tcctttcttt tagcaatttc aacagcagat aaaattacta atattaatgc aacaggaagt
      121 acagtactct ttgttaatgc ccctatacat aaagataaaa gcattaacag aaggtattta
      181 agattaatat tcttttttac aaacttaaca aaaaagtaaa tgcttaatat actaagtaag
      241 tttgcaagat tatcatagtt aatagaagat gaaagaaata caaacattaa tgtgtgtgta
      301 agaaagaata caggtaatac tcttttccat ttgtctttaa atatctcttt tgaaagaaga
      361 tacattacat aaacagttcc taaagagtag atgacattta ttattctcaa caaaacaact
      421 tcatttataa ccccattatt aatattcaat attcttccat taatccaaaa atacagatat
      481 ggaattcttg taatatctct ccattgatat gtctgtggtg tattttgtgg tatacccaat
      541 gtagtagagt aagcttgaga tactctaaga tggtagtatg agtcaggtga tactcccatt
      601 ttaatattaa aggcgagaaa tatacatacc aaaagaaaaa caaggaatat gaatattaaa
      661 gcaattttac taatatcagt cttcttcatt gtgttgtagt gaagtatacc aatttaattt
      721 gttttttggg agtagtttta ccctttttac aaagtgaaaa ataatgtaag atatactata
      781 aaatgtaagg gagagaaaaa tatgtttcta tcagatgtaa caataaaaga gtatatacaa
      841 aagggaaaaa tagaagtaaa aggtgaatat gaaatggaaa caaatgggat aagtctgcat
      901 cttggaaatg agctgctaat acaaaaacca tatcaagtag cagacagtca aaaaccacaa
      961 gaaattgaat atattaaata caacttaaag gaaagaccat atgtcctaaa acctaatgat
     1021 tttgtacttg gggcaactaa ggaattaata aaaacagata aagatataat tacaatgata
     1081 gatggaagaa gtacatatgc aagattggga atgactattc atttatctgc aatgatttta
     1141 gatggagtac cttttaatag t
//
LOCUS       DAOSAB010000182         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_83690_length_1158_cov_2.333636, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000182 DAOSAB010000000
VERSION     DAOSAB010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83690_length_1158_cov_2.333636"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PK432_03060"
     CDS             <1..340
                     /locus_tag="PK432_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59937.1"
                     /translation="KTTGNLEGFGDIKAETKVENLIRAFSSVKGREEAYNKAAKELNL
                     STYPQFSVSGGTAADWKQDILLRIDIITHKDKLDKLTEYKDKMSKFLSAEDQKAMLLN
                     EMADYFKLGQ"
     gene            492..704
                     /locus_tag="PK432_03065"
     CDS             492..704
                     /locus_tag="PK432_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59938.1"
                     /translation="MNYDSTPIERFLFKFKREHFKQELSSPVGFGAQDLEPLYLDLYL
                     DLLKEHKHKLVCKLVDFREVELWVKE"
     gene            734..>1158
                     /locus_tag="PK432_03070"
     CDS             734..>1158
                     /locus_tag="PK432_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59939.1"
                     /translation="MKKELFNKMIEKIKARPNLYSTHKLTIKDGLEVHTFGHTTNNDL
                     AEAKALFKEKGWKMNDVYDDAEKLARQNKLCNTYLTILYNADKDKISIALFETGYAFA
                     AMKERYYPLRKNVPVFAWTKHMYNLQPVGRGKHKPNPRI"
BASE COUNT          442 a          186 c          205 g          325 t
ORIGIN      
        1 caagacaaca ggcaatcttg aaggctttgg tgatatcaaa gcagaaacta aggttgaaaa
       61 tttaatcaga gctttttctt ctgttaaggg tagagaagaa gcttataata aggcagctaa
      121 agagcttaac ttatctacat accctcaatt tagtgtaagt ggtggtaccg ctgcagactg
      181 gaaacaagat atcctattgc gcattgatat tatcactcac aaggataaac ttgacaagct
      241 tactgagtat aaagataaga tgagtaaatt cttatctgct gaagatcaaa aagctatgct
      301 attaaatgaa atggcagatt atttcaaatt aggacaataa ctaattctac agagtggagt
      361 attctaaaaa gagtactccc tctttataat catttactaa cttaaaatct taacttatgc
      421 caaagaaaac taaaatcaag gacaaccatt ccggaactct tactttagct aagctgaaaa
      481 aagaggtcaa aatgaactat gacagtactc ctattgagag attcttgttc aagtttaaaa
      541 gagaacactt taaacaagag cttagctcac ctgtaggatt cggagcacaa gatcttgaac
      601 ctttgtactt agacctttac ttggatctac tgaaggaaca taaacataaa cttgtatgta
      661 agcttgtgga ttttcgtgaa gtagaactat gggtaaaaga ataaattatt aatcaactca
      721 aaaaccaaat caaatgaaaa aagaattatt caacaaaatg atagagaaga tcaaggctcg
      781 gcctaatctg tactctacac ataaattaac cattaaggac gggctagagg tccacacttt
      841 tggtcataca acaaataatg atttagcaga agctaaagct ctcttcaaag agaagggatg
      901 gaagatgaat gatgtatatg atgatgctga aaaacttgct cggcaaaata aattatgtaa
      961 tacatatctt accattctct ataatgcaga taaagataaa atatctattg ctttatttga
     1021 gaccggatat gcttttgccg caatgaaaga aagatattat cctcttagaa agaatgttcc
     1081 tgtatttgca tggacaaagc atatgtataa tcttcaacca gtgggtagag gaaaacataa
     1141 gcctaatccg agaataca
//
LOCUS       DAOSAB010000183         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_83972_length_1155_cov_2.747273, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000183 DAOSAB010000000
VERSION     DAOSAB010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83972_length_1155_cov_2.747273"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PK432_03075"
     CDS             complement(<1..476)
                     /locus_tag="PK432_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59940.1"
                     /translation="MSQNKTAPKYIILLIAVFFASVLFFLVSTPTYSQTAQELEQIIN
                     QKEEELNKHKTYLSEIEKRIKEISSSNYTLSEQINLLDAEISKLQAEISKKDKEIEEK
                     LKSIDEKQKLLNEKKESLDMVSSDLYMRSRYSEGQFLFSFSSLDQVIRSLFIKKNT"
     gene            complement(506..850)
                     /locus_tag="PK432_03080"
     CDS             complement(506..850)
                     /locus_tag="PK432_03080"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HOK59941.1"
                     /translation="MSYIVKMLSILFSNPVFARQRNELVAPNEPPRLEVLLNSLDQII
                     SYIFPIGVLAAVGMTIYGGYLWMTSGGEPDKKKRAQGTLTWSIVGLAFLYLIKMLLTI
                     VVDTITSGEIKL"
     gene            complement(888..>1155)
                     /locus_tag="PK432_03085"
     CDS             complement(888..>1155)
                     /locus_tag="PK432_03085"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HOK59942.1"
                     /translation="TSVNNLIVNILNWLIGAAAVVCVVMLIAAGYSYMTAGGDEQKVG
                     KATKTLTNALIGLAICFIAVILVNFVLENFLQSKPQSLLMTFLA"
BASE COUNT          398 a          215 c          138 g          404 t
ORIGIN      
        1 atagtatttt ttttaataaa caagctcctt ataacctgat ctaaacttga aaaagaaaat
       61 aaaaattgtc cctcactata tctacttctc atatataaat cacttgaaac catatctaaa
      121 ctttctttct tctcatttaa cagtttttgt ttttcatcaa tactttttaa tttctcctct
      181 atctctttat cctttttaga tatttctgct tgtaattttg atatctctgc atcaagcagg
      241 ttaatctgtt cagacaatgt ataattacta ctgcttatct cctttattct cttctctatt
      301 tcagataaat atgttttatg tttatttaac tcctcttctt tttgattgat aatctgttca
      361 agctcttgtg ctgtttgaga atatgttggt gtactaacaa gaaagaataa aacagatgca
      421 aagaaaacag caatcaataa aatgatatat tttggagcag ttttattttg agacattaac
      481 ttattatacc attttaggaa accttttaaa gttttatctc cccactggta atggtatcaa
      541 ctacaattgt aagaagcatc ttaattaaat acaaaaatgc taaacccaca atagaccatg
      601 taagtgtacc ttgtgctctc ttctttttgt caggttctcc accagaagtc atccaaaggt
      661 atcccccata tattgtcata ccaacagcag caagtacacc aataggaaag atataagaga
      721 taatctggtc caatgaatta agtaatacct ctaatctagg aggttcattt ggagcaacta
      781 actcattcct ctgtcgtgca aaaactggat tactaaacaa aatacttagc attttaacaa
      841 tatagctcat ctgctctata cctaatatat gaataaattt taaaaaatta agccaagaaa
      901 gtcattaata gactctgggg ttttgactgt aaaaaattct caaggacaaa attaacaagt
      961 ataactgcaa taaaacaaat tgctaaacca ataagagcat ttgttaatgt ttttgtagct
     1021 ttcccaacct tctgttcatc accacctgct gtcatatatg aatatcctgc agcaataagc
     1081 ataacaacac atacaactgc tgctgcacca ataagccaat ttaaaatgtt aactatcaaa
     1141 ttattaacac tggtt
//
LOCUS       DAOSAB010000184         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_84782_length_1148_cov_1.899360, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000184 DAOSAB010000000
VERSION     DAOSAB010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84782_length_1148_cov_1.899360"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PK432_03090"
     CDS             <1..440
                     /locus_tag="PK432_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59943.1"
                     /translation="VEYVPSKPLFRKGVIISQDSGTTLGFALTTIQDRGQLFVSPSEA
                     VYEGMIIGINNHEQDLVVNPCRARHKTNVRILRSELTEVNLKQTIPLTIEYALSFIND
                     DELIEITPKSIRLRKKLLTETERIWAKRKNLTAYAKQKMGVQD"
     gene            complement(437..>1148)
                     /locus_tag="PK432_03095"
     CDS             complement(437..>1148)
                     /locus_tag="PK432_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59944.1"
                     /translation="NLSCTPGLSETIAESSYSYLPKYERVTEMNENWGFLKEQLLSLI
                     DYRLSSSSARWYQEQYMYNKLEIDGFTPHYPSDINGLPKGIVAETLFLQASRLTQREC
                     FPCLGQEDIMGADFRITYGKQTRFFDVTVNFSERGFKKKVREGRFPSLFLPWKEEENN
                     NGYKRSYAERYLRTGTINTEEYFANIISSNYKILDRLKRFFWKGQISDVDFDYSRAGI
                     QYIKSLEGVLRLLRGCEN"
BASE COUNT          402 a          215 c          167 g          364 t
ORIGIN      
        1 atgtagaata tgtaccatca aaaccattat tcagaaaagg tgtaatcatc tctcaagata
       61 gtggtactac acttggtttt gcattaacaa caatacagga tagaggacag ctttttgtaa
      121 gtccatcaga agcagtatat gagggtatga ttataggaat aaataatcat gaacaagatc
      181 tagtagtaaa cccatgcaga gcaagacata aaactaatgt aagaatacta agaagtgagc
      241 ttactgaagt aaacttgaaa caaacaatcc cacttacaat tgaatatgct ctctctttta
      301 tcaatgatga tgagttaata gagataacac ctaagagtat aaggttgaga aaaaaacttc
      361 ttactgaaac agaacgaata tgggcaaaaa gaaagaattt aactgcatat gctaaacaaa
      421 aaatgggtgt acaggactaa ttttcacatc ctctaagaag tctaagtact ccctcaagac
      481 ttttaatata ttgaatacct gctctgctat aatcaaaatc aacatctgat atctgtcctt
      541 tccaaaagaa tctttttaat ctatcaagta ttttataatt tgaagaaata atgttagcaa
      601 aatactcttc tgtgttaata gtccctgttc ttagatatct ttcagcataa ctccttttat
      661 acccattgtt attttcctcc tctttccatg gtagaaaaag agatggaaat cttccctccc
      721 ttactttctt tttaaatcct ctctcagaga aattaacagt tacatcaaaa aatctcgtct
      781 gctttccata tgtgattcta aaatcagcac ccataatatc ttcttgacca agacaaggaa
      841 aacactccct ttgtgttaat ctacttgctt gcaaaaataa tgtctcagca acaatacctt
      901 taggcaatcc attaatatca gatggataat gtggagtaaa tccatctatt tcaagcttgt
      961 tatacatata ctgttcctga taccatctag cactactact tgagagtctg taatctataa
     1021 gggataaaag ctgctcttta agaaatcccc aattctcatt catttctgtt actctttcat
     1081 acttgggcaa gtagctgtag ctactttctg caatggtttc tgacaatcct ggtgtacaag
     1141 acaagtta
//
LOCUS       DAOSAB010000185         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_85444_length_1142_cov_2.403864, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000185 DAOSAB010000000
VERSION     DAOSAB010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85444_length_1142_cov_2.403864"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1142
                     /locus_tag="PK432_03100"
     CDS             <1..>1142
                     /locus_tag="PK432_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOK59945.1"
                     /translation="KEVSRELGIDVNVLPYIPSRVEQGYGLTESSLNDVLELDADLVI
                     TVDCGVRDRELVNRYRKEKGLEFVITDHHQPPDYLNDTLEYSLVHPMYPGHEYPFKNV
                     CGAYVAFLLVQAIKHELKMDEDISADTRGLDLVALATVTDLMLLVEVNRTVVKYGIEQ
                     IVKGERVGLNELIKVSGLNISDIDSYHLGYILGPRINAAGRIGSPIDAVKLLVSDNGQ
                     VCERIADILNETNFERQYMTEAGFNEAVNIIGESTNEKILFVASEDWHEGIVGLIAGK
                     LNEKYHRPTLVGKRNGDGVKGSARSIHGFNITEALSKCEKFLDRYGGHELAAGFTIKQ
                     GKEKEFSECIKKVAEDMITDDMLVKNLNIDLLVGSESINRELINEL"
BASE COUNT          380 a          120 c          272 g          370 t
ORIGIN      
        1 taaagaggta agtagggaac ttggtattga tgttaatgtt cttccatata ttcctagcag
       61 agttgagcag gggtatggtt taacagaatc ttcactaaat gatgtgttag agcttgatgc
      121 agatttagtt attactgttg attgtggtgt aagagataga gaacttgtaa acagatatag
      181 aaaggagaag gggttagaat ttgtaataac tgatcatcac cagcctcctg actatttaaa
      241 tgatactctt gaatattctt tagttcaccc tatgtatcct gggcatgagt atccatttaa
      301 aaatgtttgt ggagcatatg tggcttttct tcttgttcaa gctattaagc atgagcttaa
      361 aatggatgag gatatatcag ctgatacaag gggtttggat cttgtagctc ttgctactgt
      421 tactgactta atgcttcttg ttgaagtaaa tagaactgtt gttaagtatg gtattgaaca
      481 gattgttaaa ggagaaagag ttggtttaaa tgaattgatt aaagtatcag gtttaaacat
      541 aagtgacatt gacagctatc atttaggata catcttaggt ccaagaatta atgcagcagg
      601 aagaattggt tcccctattg atgcagtaaa gcttttagta agtgataatg gccaggtatg
      661 tgaaagaatt gcagatattc taaatgaaac aaattttgag aggcagtata tgacagaagc
      721 agggtttaat gaggctgtta atattattgg agaaagtact aatgaaaaaa ttctttttgt
      781 tgcgagtgaa gattggcatg agggtattgt tggtcttatt gcaggtaaac ttaatgagaa
      841 atatcacaga ccgactcttg ttggtaaaag aaatggtgat ggagttaagg gttctgcaag
      901 aagtatacat gggtttaata ttacagaagc actttctaag tgtgagaaat ttttagatag
      961 atacggcggt catgagcttg cagcaggatt tactattaag caagggaaag agaaggagtt
     1021 tagtgaatgt ataaaaaaag tagcagagga tatgattact gatgatatgc ttgttaaaaa
     1081 tttaaatata gatttattgg ttggaagtga aagtataaat agggagttaa taaatgagct
     1141 tg
//
LOCUS       DAOSAB010000186         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_85762_length_1139_cov_2.467712, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000186 DAOSAB010000000
VERSION     DAOSAB010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85762_length_1139_cov_2.467712"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PK432_03105"
     CDS             <1..807
                     /locus_tag="PK432_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59946.1"
                     /translation="YIDCCCDIHTVVIDNLTPINVCATTPPTIYPQSSSATIVLNGDC
                     DAAGPCNPPTCYCWKVTNPIIGSPDGTALFDSCTSGGPSISAFVPAGQSISNCGYYAP
                     TVSQGLIIENLGPCVNGACVPPVCTCYEIISPTPCEYSYTNCSGVNVNTVVPANTPTY
                     VCAQTVPIRFQGCPPGGTITATPYNCVNGVCTPPTPCICYLVVVSSTDGTGCIVSYTP
                     CGGAPVLQNYPDGDHYICSQVPPTQGGGAFCSVVVASTPFPCINGICQRP"
     gene            857..>1139
                     /locus_tag="PK432_03110"
     CDS             857..>1139
                     /locus_tag="PK432_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59947.1"
                     /translation="MKPLNIDKTGCSNMSSNCVVWQGPDIECINLCKGDSITEVVYKL
                     ALELCNLMDTFDLKNYDLKCFSTGVCQPQNFKDFINILINKVCALQECTN"
BASE COUNT          323 a          203 c          209 g          404 t
ORIGIN      
        1 tacatagatt gttgttgtga tatccataca gttgtaattg ataatcttac acctattaat
       61 gtttgtgcta caactcctcc tacaatttat cctcagtcaa gtagtgctac tatagttctt
      121 aatggcgatt gtgatgcagc tggtccatgt aatcctccaa catgttattg ttggaaagtt
      181 actaatccta ttataggctc tcctgatggt actgctttat ttgattcttg tacatctggc
      241 ggaccttcta taagcgcatt tgtaccagcc ggtcaaagta tatcaaattg tggatattat
      301 gcacctacag tttctcaagg tttaattatt gaaaacttag gtccttgtgt aaatggtgct
      361 tgtgtacctc ctgtttgtac ttgttatgaa ataatttctc ctactccttg tgaatacagt
      421 tatacgaatt gcagtggggt taatgtgaat acagttgttc ctgcaaatac acctacttat
      481 gtatgtgctc aaacagttcc aattagattc caaggttgtc ctccaggtgg tacaattact
      541 gctacacctt acaattgtgt aaatggcgta tgtactcctc caactccttg tatttgttac
      601 ttggttgttg ttagttctac agacggtact ggttgtatag taagttatac tccatgtgga
      661 ggagctcctg ttttacaaaa ctaccctgat ggagatcact atatctgttc gcaagtacct
      721 cctactcaag gtggtggagc tttctgttca gtagttgtgg catctacacc tttcccttgt
      781 ataaatggaa tttgtcaaag accttaaata tttgctaaag tgataaaaat taagtatatt
      841 taataataaa gaagatatga aacctctgaa tatagataaa actggctgct caaatatgtc
      901 ttccaactgt gtggtatggc agggcccaga tattgagtgt atcaatcttt gtaaaggaga
      961 ttccattact gaggttgtat acaaactggc tcttgaatta tgtaacctga tggatacatt
     1021 tgatcttaag aattatgatc ttaaatgttt ttcaacaggt gtttgtcagc ctcagaactt
     1081 taaagatttt atcaacatac ttataaacaa agtatgtgcc ctacaagaat gtacaaatt
//
LOCUS       DAOSAB010000187         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_85978_length_1137_cov_2.773567, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000187 DAOSAB010000000
VERSION     DAOSAB010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85978_length_1137_cov_2.773567"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK432_03115"
     CDS             <1..320
                     /locus_tag="PK432_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59948.1"
                     /translation="AMEEKITLFGKDWVIKKSMTAFLLYEELTGKPISQITNSLSDTL
                     TIFYCILKAKNKDFNYSFDEFIELLDDEIDALTQFNELMEKESNKLKGVVSSKKKKVR
                     IQR"
     gene            332..634
                     /locus_tag="PK432_03120"
     CDS             332..634
                     /locus_tag="PK432_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59949.1"
                     /translation="MYATAVVDGGVSPEYFWDKMDLVEYSYLVNRLNSKKIEDFEPFR
                     MLGYIISTALGSNIKYEDFLNLSKTKNGEVSDGRTPEEIELAKQKLIEIGLKHKNI"
     gene            631..>1137
                     /locus_tag="PK432_03125"
     CDS             631..>1137
                     /locus_tag="PK432_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59950.1"
                     /translation="MSILSIAAKLGLDIAGYEAGIQKVTSKNSQLNRSLEDVKSGVGA
                     LVDSLGSIGGVSSGVTGQLGEVISITNKIPDAFKNIGNGIKGFGTSITSALKAMFTSF
                     SGFISSITAAVASTGIGAIIIAIATALAGLVSWLKNSTEGADFLKSALNNLKAVIEVT
                     LTKLSTLGS"
BASE COUNT          402 a          147 c          246 g          342 t
ORIGIN      
        1 gtgctatgga agaaaaaatt acactatttg ggaaagactg ggtgattaaa aaatcaatga
       61 ctgctttttt actgtacgag gaacttactg gtaaacccat tagccaaatt actaactcac
      121 tatcagatac ccttaccatt ttttattgta tactaaaggc aaagaataag gactttaact
      181 attcatttga tgagtttatt gagttactgg acgatgaaat agatgcacta actcaattca
      241 atgagttgat ggaaaaggaa agtaacaaac taaaaggggt ggtgagttca aaaaaaaaga
      301 aggtgagaat tcaaaggtaa gtgtatatga tatgtatgca actgcggtgg ttgatggtgg
      361 tgtatcacca gaatacttct gggataaaat ggatttagtg gagtattcct acttagtgaa
      421 taggcttaat agtaagaaaa tagaggattt tgaaccattt agaatgcttg gctatatcat
      481 ttcaactgca ttgggtagta atattaaata tgaagatttt ctcaacctta gtaaaaccaa
      541 aaatggtgaa gtatctgatg gtagaacacc agaagaaatt gaattggcaa aacaaaaact
      601 aattgaaata ggattaaaac ataaaaatat atgagtatat taagcattgc tgccaaatta
      661 gggttagata ttgctggata tgaggctgga atacaaaaag taaccagtaa gaatagccag
      721 ttaaaccgta gcctagaaga tgtaaaaagt ggtgtaggtg ctctagtgga ttcacttggc
      781 tctattggtg gtgtgagtag tggagttact gggcaactag gtgaggtaat aagtataaca
      841 aataaaattc cagatgcctt taagaatata ggtaatggta taaaaggttt tggtacatca
      901 attaccagtg cattgaaggc aatgtttact tctttcagcg gttttattag ttcaattacc
      961 gctgctgttg cttcaactgg tataggtgct ataattattg ctattgctac tgcactggct
     1021 ggactggtga gttggcttaa aaactcaaca gaaggtgcag attttcttaa aagtgcttta
     1081 aataatctaa aagcagtcat tgaggtaaca ctaactaaac tgtcaacttt gggtagt
//
LOCUS       DAOSAB010000188         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_86517_length_1132_cov_2.630455, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000188 DAOSAB010000000
VERSION     DAOSAB010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86517_length_1132_cov_2.630455"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..817
                     /locus_tag="PK432_03130"
     CDS             212..817
                     /locus_tag="PK432_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59951.1"
                     /translation="MSIGNTKTNGNKGNNFAYQLAVLQLLNTISTSSSGGATEATLLQ
                     VLAALQSTQEFEQNLVIDLGGTGCPTNCPTYLQIRIWNSVTHTFGPPTYYNAAGTAVV
                     PVGPLELVNPQYTLQLILAQLTSTARTPGLLRVTTAGPGTVTAGFQKVSVYNSGVNNA
                     TLMGQTLKAGEVVSYEANKNDTLGAISYDALTSELLISTLL"
     gene            819..>1132
                     /locus_tag="PK432_03135"
     CDS             819..>1132
                     /locus_tag="PK432_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59952.1"
                     /translation="MIDQLLSSNVYQALVNASSPSAINPYITASVITSGYVPYIGATS
                     NLNLGTFSLTAGLGQFGAGTNSGRQVEIFQDSANVKIGSLVGTPTSSAIYLNQTTPSL
                     TN"
BASE COUNT          407 a          174 c          179 g          372 t
ORIGIN      
        1 gaataagttc cactaaaaga aggattgcga cattttactt ttactaataa tacgtttcag
       61 aaaaatggag ctaatcattt tgctacaggc ttggtaatag aatgggttaa taatacatca
      121 tttaatattt taactatcaa ttcagaaact aaatctttat ctgatagttt attagataaa
      181 acttctttaa ctatttataa atacgattaa catgtctata ggaaatacaa aaacaaatgg
      241 taacaaagga aataactttg cttatcaatt agctgttttg caactattaa atacaatatc
      301 tactagttca tctggtggag ctacagaagc aacattatta caagtacttg ctgctttaca
      361 atctactcaa gaatttgaac aaaatttagt aatagatctt ggtggaactg gttgtccaac
      421 taattgtcct acatatcttc aaatcagaat atggaattca gtaactcata cttttggacc
      481 tcctacatat tataatgctg ctggtacagc agttgtacct gtaggacctt tagaattagt
      541 taatcctcaa tatactttac aattaatatt agcacaatta acttctacag caagaactcc
      601 tggattattg agagtaacta cagctggtcc tggaacagtt actgctggtt ttcaaaaagt
      661 atctgtatat aatagtggtg taaataatgc tacattgatg ggacaaacct taaaagctgg
      721 agaagtagtt tcttatgaag ctaataaaaa tgatactttg ggtgctatat cttatgatgc
      781 attaacaagt gaattattaa tctcaacctt attataaaat gatagatcaa cttttatcta
      841 gtaatgtgta tcaagcattg gttaatgctt catcacctag tgctattaat ccttatataa
      901 cagctagtgt tataacatca ggatatgttc cctatatagg agcgacatca aatttaaact
      961 taggaacatt tagtttaaca gcaggattag gtcaatttgg tgctggcact aatagtggaa
     1021 gacaagttga gatatttcaa gatagtgcaa atgtaaagat tggtagttta gtaggcacac
     1081 cgacatcaag tgcaatctat ttaaaccaaa caacaccaag tctaacaaat ta
//
LOCUS       DAOSAB010000189         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_87776_length_1121_cov_2.037523, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000189 DAOSAB010000000
VERSION     DAOSAB010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87776_length_1121_cov_2.037523"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..409)
                     /gene="rplX"
                     /locus_tag="PK432_03140"
                     /pseudo
     CDS             complement(110..409)
                     /gene="rplX"
                     /locus_tag="PK432_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
     gene            complement(426..794)
                     /gene="rplN"
                     /locus_tag="PK432_03145"
     CDS             complement(426..794)
                     /gene="rplN"
                     /locus_tag="PK432_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOK59953.1"
                     /translation="MIQRESNITIADNSGAKVGKVIGASGYSKIKFIGLGSTVKIAVQ
                     KTLPNSGVKKHDVLTAVIIRVTKEHKRKDGTYIRFDDNAAVIIDNKKNPVGTRIFGPV
                     ARELKEKGFDKIVSLAPEVL"
     gene            complement(797..1057)
                     /locus_tag="PK432_03150"
     CDS             complement(797..1057)
                     /locus_tag="PK432_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uS17 family ribosomal protein"
                     /protein_id="HOK59954.1"
                     /translation="MEKKKKNKNKNKKKQFEGVVVSNSMQSTVRVRVDTLQSHPVYRK
                     VVNRRKVFFAHTDKELNVGDKVVIRESRPYSKNVKWVVVNTK"
BASE COUNT          296 a          243 c           97 g          485 t
ORIGIN      
        1 cttgccatta ttctttttta gtttttgatt tagatttact ttttacagtt ttcttaactt
       61 ctttcttttc ttctttcttt tcttctttta cttcttcctt agcaatgatc tcaataactt
      121 taccgcattt tttacatatt ctttcaactt taccattctc tcttctaatc cccaccctag
      181 ttggcttact gcagttagga catacaagca taactttaga cacatccata ggtttagcaa
      241 tatctattat tgcagacttt ttttcttttc catcaccctt tacatgtttt ttgtacatat
      301 ttaaaccatc tataataact ttcccattct ttacagcaga aacaacacct tgttttccag
      361 tgtccttccc ataaagtatt tttactgtat ctcccttttt aatcttcata tatttaattc
      421 ataaattaca aaacctcagg agcaagagaa actattttat caaatccttt ctcttttaat
      481 tctcttgcta ctggaccaaa tattcttgtt ccaacaggat tcttcttatt atcaattatt
      541 actgccgcat tatcatcaaa tcttatatat gtaccatctt tccttttatg ctcttttgta
      601 actctaataa ttacagcagt taagacatca tgcttcttaa ctccgctatt gggcaaagtt
      661 ttctgtactg caattttaac agtacttcca agaccaataa atttgatctt agagtaccca
      721 gaagctccta ttacctttcc tacttttgct ccactattat ctgcaattgt tatattactt
      781 tctctctgta tcatttttat ttagtattaa ctacaaccca tttaacattc ttagagtagg
      841 gtcttgattc tcttattact accttatctc caacatttaa ttctttatct gtatgtgcaa
      901 agaatacctt ccttctattt acaaccttcc tgtatacagg atgagattgt aaggtatcaa
      961 ccctaactct tacagtgctt tgcatactat tgcttacaac tactccttca aattgtttct
     1021 ttttattttt atttttattt ttctttttct tttccatatc tctttttaat atttaaaact
     1081 gctagtattc tatcaatatt aagcaaatct aacaagccta t
//
LOCUS       DAOSAB010000190         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_88179_length_1117_cov_3.324859, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000190 DAOSAB010000000
VERSION     DAOSAB010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88179_length_1117_cov_3.324859"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1117)
                     /locus_tag="PK432_03155"
     CDS             complement(<1..>1117)
                     /locus_tag="PK432_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59955.1"
                     /translation="EEAERQYAAKILETGLKDITKKLDKTKFEYNSLNERINQLQTKA
                     QTSALTPEEQQILDNALSNRDIKQSEKESYENSINAIKANADAGDMPTIRRRVDGAIA
                     QGLLENDLSELAYSLSAVGEEKIVANPIREIKLRHQSQLALASANHNYNIIELEKQHD
                     LKLKEIEYKALVEQVQGSTDGGGGAGYSQGFGEAPSYKEAGAGTNIELPNKEDAYLYT
                     KSEVEKSVNNARAGSLSFLYTAFDRAKQAALSPNGSVTATKWLKDVVGNDWKNISDQE
                     TFNQIMVNNEATKSKSVGVLYQQTLNMFKNNKTGGIGWGEDLLTSNALLLNNIKVAQQ
                     AANGELAKQVQNNLQIATSLKATAGSNPLYKYVDELF"
BASE COUNT          314 a          202 c          155 g          446 t
ORIGIN      
        1 aaaaataatt catcaacata tttatacaaa ggattacttc ctgccgtagc ttttaggctg
       61 gtagcaatct gcaagttgtt ttgaacttgt ttagctaatt caccattagc tgcttgttga
      121 gctactttta tattatttaa taatagtgca tttgatgtaa gtaaatcttc tccccaacct
      181 ataccaccag tcttattatt tttaaacatg tttaatgttt gttgataaag aacacccaca
      241 gatttgcttt tagtagcttc attattaacc attatttgat taaaggtttc ttgatcagat
      301 atatttttcc agtcatttcc tacaacatct tttaaccatt tggtagctgt tacagaacca
      361 ttaggtgaaa gagcagcttg tttagctcta tcaaatgctg tatacaaaaa acttaaagaa
      421 ccggctctag cattattaac agatttttca acctctgatt ttgtatataa gtatgcatct
      481 tctttattag gaagttctat attagttcct gcaccagcct ctttataaga aggggcttct
      541 ccaaatcctt gactatatcc ggctcctcca cctccatcag tagatccttg tacttgctct
      601 actaaagctt tgtactctat ttctttaagc tttaaatcat gttgtttttc tagttcaatt
      661 atattataat tatgattagc agatgctaaa gctaattgag actgatgtct tagttttatt
      721 tctcttatag gattagcaac aattttttct tcacctacag cagataaaga ataagctagt
      781 tcacttaaat cattttcaag taatccttga gctatagcac catctactct tcttctaata
      841 gtaggcatat ctccagcatc agcatttgct ttaatagcat ttatagagtt ttcataactt
      901 tctttttcag actgtttaat atctctgtta gaaagagcat tgtctaaaat ctgctgttct
      961 tctggagtta atgctgaagt ttgtgctttt gtttgaagtt gatttatcct ttcattaagg
     1021 ctattatatt caaacttagt tttatcaagt ttctttgtta tatctttaag accggtttca
     1081 agaatttttg cagcatactg tctttctgct tcttcta
//
LOCUS       DAOSAB010000191         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_88246_length_1117_cov_1.763653, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000191 DAOSAB010000000
VERSION     DAOSAB010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88246_length_1117_cov_1.763653"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1117
                     /locus_tag="PK432_03160"
     CDS             <1..>1117
                     /locus_tag="PK432_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59956.1"
                     /translation="VTYPNVPNLNGNCIIANLTFTDSRGCTDSITTTCTPCSPTVSFK
                     CDNGNCYDPGDGTGTHTTENACKNSCPCTTMTLDNVTGCLANNTVNIVPTFTAACNDI
                     NNNIAVGNIVSITDDDNPSNSWIITSNPYILTYPQGIVPGTYNLTANWVCGVCTGNTQ
                     FTVTVTTVLITVSDITNDCNAQSPLSVNLYSAIQGGNSANTIFRIDTILDGSVTCTSF
                     GTLTGGTLLNINTWTTVGTNTIYYEYTDSTTQCKKCGSFEVTINDCCTNSMTIGDVSG
                     CLSDGSVNINAAFAEACVGGGITALVENNALSNTWGIIGSYPNYTISYPPSIIPDIYE
                     LTATWECGTCTDITEFNIIVNPNPSVTTTSYTGVCPFD"
BASE COUNT          376 a          172 c          193 g          376 t
ORIGIN      
        1 gtaacttatc caaatgtgcc taatttaaat ggtaattgca taatagcaaa cttaactttt
       61 acagatagta ggggttgtac agatagtata actacaacat gcacaccatg ctctccgaca
      121 gtatctttta agtgtgataa tggtaattgt tatgaccctg gtgatggtac aggtacacat
      181 acaactgaaa atgcttgtaa aaattcatgt ccttgtacta caatgacact tgataatgta
      241 actggttgtc ttgctaataa tacagtaaac atagtaccta cttttacagc agcttgtaat
      301 gatattaata ataatatagc tgttggaaat atagtaagca taactgatga tgataatcct
      361 tctaatagtt ggattataac atctaatcct tacatattaa cctatccaca aggtattgtt
      421 ccaggaacat ataacttgac agctaattgg gtatgtggtg tatgtactgg taacacacag
      481 tttactgtaa ctgtaactac tgtactcatt acagtatctg atattactaa tgattgtaat
      541 gctcaatcac ctttgtctgt taatttatat agtgctatac aaggtggaaa tagtgctaat
      601 actattttta gaatagacac tattttagat ggttcagtta cctgtacttc ctttggtaca
      661 ctaacaggtg gtactttact aaatatcaat acttggacaa cagtaggtac taatactata
      721 tattatgaat atacagatag tacaacacaa tgtaaaaaat gtggtagctt tgaagtgacg
      781 ataaatgatt gttgtactaa ctcaatgacg attggtgatg tatcaggctg tctttccgac
      841 ggttcagtta atataaatgc tgcatttgca gaagcttgtg ttggtggagg aataacagcc
      901 ttagtagaaa ataatgctct ttctaatact tggggaataa ttggatcata tccaaattat
      961 acaataagtt atccaccaag tattattcca gacatatatg aattgacagc tacttgggag
     1021 tgtggaacat gtacagatat tacagagttt aatataattg taaatcctaa tccatcagtt
     1081 actacaacat cttatacagg tgtatgtcca tttgatt
//
LOCUS       DAOSAB010000192         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_89127_length_1108_cov_3.562203, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000192 DAOSAB010000000
VERSION     DAOSAB010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89127_length_1108_cov_3.562203"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="PK432_03165"
     CDS             complement(<1..1086)
                     /locus_tag="PK432_03165"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOK59957.1"
                     /translation="MLPLISDIEVVETNNEIEALVLESALVRKFKPKFNSNLKDDKSY
                     AWIYINTQDRFPTVKIVRTIRKGEYKKGRLFGPYPSGYTIKRVYSYLRKLYPFCTCKN
                     HDCASSLYYHIGLCPGPYIGAISEEDYRKNINNIIKFLSGKQQDHIKRLEKEMKEYSK
                     RQQYEKASQLRDRIKDLKYISQDIDFTYYDDALSYESRRDKARKISFNYLGMELHIPN
                     LHRIECFDISNIQGKNAYGSMVVAEDGKLNRSEYRIFKIKGEDKPNDYKMLREVLERR
                     FKDKDKKVDLVLIDGGKGQLNVVKDVIHRKVSLMGISKGRYLKRKGKNSVDEFWILKD
                     GNVYRIDIASPEILIDLRNEAHRFAISY"
BASE COUNT          318 a          205 c          125 g          460 t
ORIGIN      
        1 atatgatatt gcaaatctat gtgcctcatt cctaagatct atcaatattt ctggacttgc
       61 tatatctatt ctatatacat ttccatcttt taatatccaa aattcatcaa cagagttctt
      121 tccttttctt tttaggtatc taccttttga gatacccatt aatgatactt ttctgtgaat
      181 aacatctttt actacattca actgtccttt tcctccatca attaatacaa gatcaacctt
      241 tttgtcttta tctttaaatc tcctttcaag tacctctcta agcattttat aatcgttagg
      301 tttatcctct cctttaatct taaatatcct gtattcagat ctgttcaact tcccatcttc
      361 tgctactacc atagagccat atgcattttt accttgaata tttgatatat caaaacattc
      421 aattctatga agattaggaa tgtgtaattc cataccgaga tagttaaagc taatctttct
      481 tgctttgtct cttctgcttt catatgagag agcatcatca tagtatgtaa aatcaatatc
      541 ttgactgata tattttaaat cttttattct atctctcagt tgagaagctt tttcatactg
      601 ctgtcttttt gaatattctt tcatctcttt ttcaagtctt ttaatatgat cctgctgttt
      661 gcctgaaaga aatttaataa tgttattaat attttttcta taatcttctt ctgatattgc
      721 accaatatat ggaccaggac ataaacctat atgatagtaa agagaagatg cacaatcatg
      781 atttttacat gtacaaaatg gatacaactt ccttagatat gaatatactc ttttaattgt
      841 atatccacta ggatatggac caaacaatct tcctttttta tactctccct ttcttatcgt
      901 tcttacaatt tttacagtag ggaatctgtc ttgtgtgttt atatatatcc atgcatatga
      961 tttatcatct ttaagattac tattaaactt tggtttaaac tttcttacaa gtgcagattc
     1021 cagcactaat gcttctattt cgttgtttgt ttcaacaact tctatatcag atatgagggg
     1081 gagcattttt actatatatg gtctatca
//
LOCUS       DAOSAB010000193         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_90672_length_1096_cov_2.557157, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000193 DAOSAB010000000
VERSION     DAOSAB010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90672_length_1096_cov_2.557157"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1096
                     /locus_tag="PK432_03170"
     CDS             <1..>1096
                     /locus_tag="PK432_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59958.1"
                     /translation="VLVGTTSVEKSEILSDMLSKEGIKHEVLNAKYHEKEAKIVAQAG
                     QKGAVTIATNMAGRGTDIALGEGVRELGGLYIIGSERHESRRIDNQLRGRSGRQGDPG
                     ESVFYVSFEDDLMRLFGGDRMQMIMASVGLDDNTPISMGILGRTIENAQKKVESHNYD
                     IRKHLVEYDDVLNQQRNIIYDLRTKVLEISKQPYVESKVDLEKDIKYILSIDIDRLQD
                     ELDSFSLTRKETWDISLLNDLKFKLTPLDIFVLKKILEHIDFLISSQLEDDMKIDNIE
                     ERNLYIQIKNLITDEIFEKATKRLKFKDSVEYFRDLYGKDSAQRGSQLKKLTLVAFVE
                     HIYGIGTLPMRELSKVLILQSIDRYWMEHLD"
BASE COUNT          379 a          130 c          247 g          340 t
ORIGIN      
        1 gtacttgtag gtactacttc tgttgagaag tctgaaattc tctctgatat gctttctaaa
       61 gaaggaatta agcatgaggt attaaatgct aaatatcatg agaaagaggc taagatagtg
      121 gctcaggcag gtcagaaggg tgctgtaacc attgcaacca atatggcagg aagaggaaca
      181 gatattgcac taggagaggg tgtaagggag cttggagggc tgtatataat cggttcagaa
      241 agacatgaat caagaagaat agataatcag ttaagaggaa gatctggaag gcagggagac
      301 cctggtgaat ctgtcttcta tgtttctttt gaagatgacc ttatgagact ttttggaggt
      361 gataggatgc agatgattat ggcaagtgta gggcttgatg ataacacccc tatatccatg
      421 ggtattttag gaagaactat tgagaatgca caaaagaagg ttgaatctca taactatgat
      481 attagaaaac atcttgttga atatgatgat gttcttaatc agcagagaaa cataatatat
      541 gatcttagaa caaaggtttt agaaatatca aaacagccgt atgttgaaag caaggttgat
      601 ttagagaagg atattaaata tatactgtct atagatatcg acagactcca agatgagctt
      661 gatagttttt ctcttactag gaaggaaact tgggatatat cgcttcttaa tgatttaaag
      721 tttaagctta ctcctcttga tatatttgta ttaaagaaaa ttttagaaca tattgatttc
      781 cttatatctt cgcagcttga ggatgatatg aagatagata atattgaaga aagaaacttg
      841 tatattcaga ttaaaaatct cattacagat gagatatttg aaaaagctac taaaaggctt
      901 aagtttaagg atagtgttga gtattttaga gatttgtatg gtaaagattc tgcacaaagg
      961 ggttctcaat tgaagaaact tacacttgta gcatttgttg agcatatata tggtattggt
     1021 acacttccaa tgagagagtt gtctaaggtt ttaatacttc agagtattga tagatactgg
     1081 atggagcatt tagata
//
LOCUS       DAOSAB010000194         1092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_91143_length_1092_cov_3.577628, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000194 DAOSAB010000000
VERSION     DAOSAB010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91143_length_1092_cov_3.577628"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK432_03175"
     CDS             complement(<1..370)
                     /locus_tag="PK432_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59959.1"
                     /translation="MNEIAREILEKRSGEPAREKVRRLFGKGGLTEDYARKMLAGMRL
                     YIEKTEGLSDKVRGEDTVKYYRDGTREYVRDISLSDNEAADPATVMRKMGFDPLLWEL
                     IDCKLRKGNWDSTIKNNDGDP"
     gene            complement(363..800)
                     /locus_tag="PK432_03180"
     CDS             complement(363..800)
                     /locus_tag="PK432_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59960.1"
                     /translation="MSENSLKKAVLESRASAAEAEAMSTELKKSKVITLRVEEPLFKA
                     IEAQAELWKVKPAETIRRVLWFYFLPAALEMQLRGESEKFWKGELTPEAAGEYMVFTL
                     EAVEKLRSSAMFLSREALRLREAMEGKLSEAVEIVRKALEHSE"
     gene            complement(869..>1092)
                     /locus_tag="PK432_03185"
     CDS             complement(869..>1092)
                     /locus_tag="PK432_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59961.1"
                     /translation="ALEGNVFLVEGEKYAIRERKKVKGKPKFYLIRCEPFQYVSSLFP
                     VEPEEFRFDYEKRLYSLKKKGNMVAIREL"
BASE COUNT          277 a          303 c          157 g          355 t
ORIGIN      
        1 caggatcgcc atcattgttt ttgattgtgc tatcccagtt gcccttgcgc agtttgcaat
       61 ctataagctc ccacaacagt gggtcaaagc ccatttttct cataacagtt gctgggtctg
      121 cagcttcgtt atccgataag cttatatccc ggacgtattc tcgcgtcccg tcgcgataat
      181 acttgacagt gtcttcgccc ctaactttat cagataaccc ctctgttttt tcaatgtaaa
      241 ggcgcatccc ggcaagcatt tttcttgcgt aatcttctgt taaaccccct ttaccaaaaa
      301 gacgccggac tttctctcgt gccggctctc ctgaacgctt ctctaatatc tcacgcgcaa
      361 tttcattcac tgtgctccaa cgcctttctc acaatctcaa cagcttcact tagctttccc
      421 tccattgcct ctctcaacct taaggcttct ctacttaaaa acattgcaga gcttctaagt
      481 ttctcaactg cttccaaagt aaataccatg tattctcctg cagcctcagg agttaactca
      541 cctttccaga acttctcact ctcacctctc aactgcattt caagcgcagc aggtaaaaag
      601 taaaaccaga gaactctcct tatagtttca gcaggtttta ctttccagag ctcagcttga
      661 gcttctattg ctttgaaaag aggctcttca actctaagtg ttataacctt gctctttttc
      721 agttcagtgc tcatagcctc agcctcagct gctgaagctc tgctctcaag aactgccttt
      781 ttcaaactgt tttcactcat acaaactcct ttgcatgtaa gcaaattata ccacttttgt
      841 ttaccacttc taagtaaaaa aaataaactt atagctctct aattgcaacc atgtttcctt
      901 ttttcttcag agagtaaagc cttttctcat aatcaaatct gaactcttca ggttctactg
      961 gaaacaagct gctcacatac tgaaaaggtt cacaccttat caggtaaaac ttaggttttc
     1021 ctttcacttt tttcctctct ctgatagcat acttctcacc ttcaaccaga aaaacattgc
     1081 cttccagagc aa
//
LOCUS       DAOSAB010000195         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_92973_length_1078_cov_2.558162, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000195 DAOSAB010000000
VERSION     DAOSAB010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92973_length_1078_cov_2.558162"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(413..>1078)
                     /locus_tag="PK432_03190"
     CDS             complement(413..>1078)
                     /locus_tag="PK432_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOK59962.1"
                     /translation="MILEHIVDCVLYIEGGEFNQYRIIRGMKNRFGSTNEIGVFEMTS
                     AGLNEVGNPSLVFLDTKSSVSGSAISAVVQGSRVVFVEVQALVVERGAEVGPLRRVAN
                     GIKKPRLDMLCAVLSRRGNVYLGDKDVFVNVVGGITVSDPAVDLAVCAAIKSVVRDEV
                     LDKKYIYFGEVGLTGEVRGTWGLDSVMAEASRVGYKGIYVGNAKIKKSSMEIKVVENI
                     RKL"
BASE COUNT          368 a          257 c          118 g          335 t
ORIGIN      
        1 aattgatttt agtcaaataa aaaaagaaaa ggggatcggt actgccccaa ccgaccccgg
       61 tattatccat taactattgt ttatggataa aaccgacgag catatagatg cttcccctta
      121 atattctatc ataaatatat aaatatctta accttttcct tttgcccaga tgtgtttgtg
      181 gcaccccaga caaagggaga aagttaagat atccacatac tatgttaaat gaacaacata
      241 ttataaccct agtgtatatg atatgtcaac cccataagaa gtgtccacat ataaacacat
      301 atacagattc ttgtttagga acaccacttt gaaccattta atctttaata tgttacgata
      361 tggataatca aaagtatatg ttacgatttt tgtggatgat atttgtaatt agttaaagtt
      421 ttcttatatt ttcaacaact tttatctcca tactactctt ttttatcttt gcattgccaa
      481 cataaatgcc cttatatcct acccttgaag cctctgccat tacactatca agtccccatg
      541 tacccctaac ctctcctgta agaccaacct ctccaaagta aatatacttt ttatcaagca
      601 cttcatctct tacaacagat ttaattgcag cacaaactgc taaatcaaca gcaggatctg
      661 aaacagtaat accacctaca acattaacaa acacatcctt atctcctaaa tacacattac
      721 ctctccttga aagtactgca cacaacatat ccaacctagg tttctttatg ccatttgcaa
      781 ctcttcttaa aggtccaacc tctgctcctc tttctacaac aagtgcctga acctcaacaa
      841 aaacaaccct tgaaccttga acaactgcac taatagcact accagataca gaactttttg
      901 tgtcaagaaa aactaaagaa ggattcccta cttcatttaa acctgcagaa gtcatttcaa
      961 aaacaccaat ttcatttgta gaaccaaatc tattcttcat tcccctaatt attctatact
     1021 gattaaactc tcccccctct atgtacaaga cacaatctac aatatgctct aaaatcat
//
LOCUS       DAOSAB010000196         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_93834_length_1071_cov_3.405512, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000196 DAOSAB010000000
VERSION     DAOSAB010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93834_length_1071_cov_3.405512"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1071)
                     /locus_tag="PK432_03195"
     CDS             complement(<1..>1071)
                     /locus_tag="PK432_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK59963.1"
                     /translation="NPYFRRGGVAREWIYGYTYILSHFAKNLNEKVAEERDIYGIGHD
                     EEIESLISVVGKVSNRNALLIGEPGVGKSSIVLGIAQRINSGDVPPQIRDKRILQLDV
                     NGLIALSSKEHNMESLISKAMKELENAGDVILYIDEMQELIPSKASESGHSIAGILLP
                     YILNSKFPVVGTVNYADYKKYFYSMESLRQSFTNIEIKEISAVDALFILETKVSQLER
                     NFGIYLTFPALISAVELAQRYNRERKLPSSAVQLLEETCSWAQANGIKVITGEHVSKV
                     LSNQKGIPIGSINPQETTKLMKLEENMRKQIIGQDEAVKSLVEALTRAQIDIRDPNRP
                     IGTFLFLGPTGVGKTHISKVLAQ"
BASE COUNT          277 a          223 c          167 g          404 t
ORIGIN      
        1 ttgtgcgagt actttagaaa tatgtgtttt acccacacca gtaggtccca agaagaggaa
       61 tgttcctatt ggcctgttgg gatctcgtat atctatctgc gctcttgtaa gggcttctac
      121 taacgatttt acagcctcat cttgtcctat tatctgcttt ctcatatttt cttctagctt
      181 cattagcttg gttgtctctt gtggatttat actcccaatt ggaatacctt tctgatttga
      241 aagaactttt gatacatgtt ctcctgttat taccttaatt ccatttgctt gtgcccatga
      301 gcatgtttct tcaagaagtt gaactgcact gcttggaagc tttctttccc tgttatatct
      361 ttgtgcaagc tctacagcac taattaaagc aggaaatgtg aggtatattc caaagtttct
      421 ttctagttgt gatacttttg tttcaagaat gaacagtgca tcaacagcag agatttcttt
      481 aatctcaata ttagtaaagg attgcctaag ggattccatg ctgtaaaagt attttttata
      541 atctgcatag tttactgtac ctacaacagg aaacttactg tttaatatat atggaagaag
      601 tatacctgca atagaatggc cactctcact tgcttttgaa ggaattagct cctgcatttc
      661 atcaatgtag agaattacat ctcctgcatt ttctagttcc ttcattgcct tagaaattaa
      721 ggactccatg ttatgttctt tagatgacag agctattagt ccgtttacat caagctgtaa
      781 gattctttta tcccttattt gtggcggaac atctccagag tttattcttt gagcaattcc
      841 tagtactatg ctggacttac ctactccagg ttctccaatt aacagtgcat tcctatttga
      901 tacttttcca acaacagaga ttaagctctc tatttcttca tcatgcccta ttccatatat
      961 atccctttcc tctgctactt tttcatttag attctttgca aaatgactta atatgtatgt
     1021 atatccatat atccactccc ttgcaacccc tcctcttcta aaatatggat t
//
LOCUS       DAOSAB010000197         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_95820_length_1056_cov_2.631369, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000197 DAOSAB010000000
VERSION     DAOSAB010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95820_length_1056_cov_2.631369"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1056)
                     /gene="groEL"
                     /locus_tag="PK432_03200"
     CDS             complement(<1..>1056)
                     /gene="groEL"
                     /locus_tag="PK432_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006802540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOK59964.1"
                     /translation="AKSFGSQVVTKDGVTVAKEIEEKDPFKNVGVEMLKEAARKVNDT
                     AGDGTTTVTVLAQAIANAGFKNVTAGANPISLKRGLDKAVDIVVEELVKSSKKIKTQE
                     EISQVATISSNNDKELGDMIGKVFEKIGKDGVITVEEAKGFDDEVEYTEGMQFDQGYV
                     SAYFVTDPETLEAVMDNPYIFVTDKKLSNLQDIQAIAEKVLAAADRPLVIIADDIENQ
                     ALATLVVNKLRGTLNVVAVKAPGFGDRKKEMLKDIAVVTGAEFVSEEVGRTLDSVSLS
                     DLGGAKKVIVSKEETVIVEGSGNQDEIKKRINEIKAQIENTTSDYDREKLQERLAKLS
                     GGVAVVKVGAASEVEAK"
BASE COUNT          304 a          264 c          123 g          365 t
ORIGIN      
        1 ccttggcctc tacttcagaa gcagcaccaa cctttacaac agcaacacca ccagataact
       61 tagctaatct ctcttgcaac ttctctctgt cataatcaga ggtagtattc tcaatctggg
      121 ctttaatctc atttattctc ttcttgattt catcctgatt tccactccct tctacaataa
      181 cggtctcttc tttacttact attactttct ttgcaccacc taagtcagac agacttacag
      241 aatcaagtgt tctaccaact tcctcagaaa caaattcagc acctgttaca actgcaatat
      301 ctttaagcat ctcttttttt ctatcaccaa atccaggagc cttaactgct acaacattaa
      361 gagttcctct tagtttatta acaactaatg tagctaatgc ttgattctct atatcatctg
      421 caataattac aagaggtctg tctgcagcag caagtacttt ctctgcaata gcttgaatat
      481 cctgtagatt agaaagcttt ttatctgtaa caaatatgta tggattatcc attacagcct
      541 caagtgtctc tggatctgtt acaaagtatg cactaacata cccctgatca aactgcatac
      601 cttctgtata ttcaacttca tcatcaaaac cttttgcttc ttctacagta atcactccat
      661 ctttccctat cttctcaaat actttaccaa tcatatctcc aagctcttta tcattatttg
      721 aagatattgt agcaacttga gaaatctcct cctgtgtctt tatcttttta gaagatttaa
      781 ccaactcctc tacaacaata tctacagcct tatccaaacc tcttttaaga gagatagggt
      841 tggcacctgc agtaacattt ttaaaacctg cattagcaat agcttgagcc aaaacagtta
      901 ctgtagtagt tccatcacct gcagtatcat ttacctttct tgcagcctct ttaagcatct
      961 ctacacctac attcttaaaa ggatcttttt cttcaatctc ttttgctaca gttactccat
     1021 ctttagtgac aacttgagaa ccaaaagatt ttgcca
//
LOCUS       DAOSAB010000198         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_95845_length_1056_cov_2.177822, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000198 DAOSAB010000000
VERSION     DAOSAB010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95845_length_1056_cov_2.177822"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1056
                     /locus_tag="PK432_03205"
     CDS             <1..>1056
                     /locus_tag="PK432_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOK59965.1"
                     /translation="LTLYLYALTGRGAHLVTVNDYLAKRDAEWTGHILNSLGMTTSAI
                     NSGTQYRFISDEEAIKLKGDEAKKLIDERNRKVKEEGRLKYDHMSGVNLIECTKREAY
                     ACDVVYGTNNEFGFDYLRDNMVSDLDDRVQGPRYFAIVDEADSILIDEARTPLIISTS
                     AQASNEMYKQFASLVNQLQREKHYSVDEKSNSVSLTDEGVDYVEKLLKVKNIYEDPQI
                     AYHLDNALKAKELYKRDDEYMVKDGEVLIVDEFTGRVLPGRRYSEGLHQAIEAKEGVE
                     VKRESRTMATITLQNYFRLYFYLSGMTGTALTEAEEFASIYGLDTIVIPTNKPVIRKD
                     YNDVVYRTQIGKFKAIV"
BASE COUNT          348 a          134 c          250 g          324 t
ORIGIN      
        1 ccttactctt tatctttatg ccttaactgg caggggtgct catttagtta ctgttaatga
       61 ttatcttgct aaaagagatg ctgagtggac aggacatata cttaatagtc tgggaatgac
      121 tacttctgca attaattctg gtacacagta caggtttatt tctgacgaag aagctataaa
      181 gctgaaaggg gatgaagcta agaagttaat tgatgagaga aacagaaagg taaaggaaga
      241 gggaagactt aagtatgacc atatgagtgg tgttaatttg attgagtgta ctaaaagaga
      301 ggcatatgct tgtgatgttg tatatggtac taacaatgaa tttggttttg attatcttag
      361 ggataatatg gtttctgatt tagatgacag agtgcaggga ccaagatact ttgcaatagt
      421 tgatgaggca gactctattc tcattgatga ggcaagaact cctttgatta tttctacaag
      481 tgctcaggca tcaaatgaga tgtataaaca gtttgcgagt cttgtgaatc aactccaaag
      541 agagaagcat tactctgttg atgaaaaatc taattctgta tctcttacag atgagggcgt
      601 ggattatgta gagaaacttc ttaaggttaa gaatatatat gaagatcctc aaattgcata
      661 tcatttagat aatgcactta aagcaaaaga gctttacaaa agggatgatg agtatatggt
      721 taaggatgga gaggttttaa ttgttgatga atttacaggg agagtacttc ctggaagaag
      781 gtatagtgag ggattgcatc aggcaattga agcaaaagag ggtgtagagg taaagagaga
      841 gagtagaact atggcaacta ttacactaca gaactatttt aggctttact tctatctttc
      901 tggtatgacg ggtactgctc ttacagaggc tgaggagttt gcctctatat atggtctaga
      961 taccattgtt ataccaacaa ataagcctgt tataaggaag gattacaatg atgttgttta
     1021 cagaactcaa attgggaagt ttaaagctat tgtaga
//
LOCUS       DAOSAB010000199         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_99239_length_1032_cov_3.234391, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000199 DAOSAB010000000
VERSION     DAOSAB010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99239_length_1032_cov_3.234391"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..>1032
                     /locus_tag="PK432_03210"
     CDS             142..>1032
                     /locus_tag="PK432_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59966.1"
                     /translation="MKKILLITCTLGMLLFLPTLSFANTNSIPLNQENCIDLEEIYKQ
                     INKSSQAVTGVDNSIDAVKKVNTVASLMNVISLFLGEQIYCVKDEEAALQSNTLKSVG
                     LFGMLNNGTNTLISMYPDINVSDHLATMFVPGYKENNTTFAQTIIDPGDLELGGNRTA
                     QNDAKKRTNNIIEKAVKPELEKTVQRYSDSIKIEEINMEETPTPSISGYNYLKDVVHL
                     DKIWRSSLNIVYLFSIIIFIIVGFMIMFRKNLPGNTTVTVSKALPQIIISLVLATFSF
                     AIVGIIMDLGKVGINLSRSII"
BASE COUNT          410 a          155 c          170 g          297 t
ORIGIN      
        1 acaaaactaa aggatattgc atatatgaag attatgcgag aatatattac aatagtaaga
       61 ggtatggaga aactagatgc tttggtgaaa cagacttata ctgtgattac gaaaaatata
      121 taattaatat acattaaaaa tatgaaaaaa atactgctca ttacttgtac tctagggatg
      181 cttctctttc ttcctacatt atcatttgca aatactaact ctatcccatt aaaccaagaa
      241 aactgtattg acttagagga gatatataaa caaattaata agtcaagtca agcagtaaca
      301 ggtgttgaca atagtataga tgcagtaaaa aaggtaaaca cagtagcatc actgatgaat
      361 gtaatctctc tcttccttgg agaacaaata tattgtgtca aagatgagga agctgcactt
      421 caatcaaata ctcttaagag tgtaggattg tttggaatgt taaacaatgg aactaatact
      481 ctaataagta tgtacccaga tattaatgta agcgaccact tagcgacaat gtttgtacct
      541 ggatataaag aaaacaatac aacatttgca caaacaataa tagatccagg ggacttagag
      601 ctaggaggaa ataggacagc acaaaatgat gcaaaaaaac gtacaaataa tattatagaa
      661 aaagcagtta agccagaact agaaaagaca gttcaacgat actcagactc cattaagata
      721 gaagaaatta atatggagga aacccctact ccttccatca gtgggtacaa ctatcttaaa
      781 gatgttgtac atctcgataa aatatggaga tcctctctca acatagtata tctattttca
      841 attatcatat tcataatagt tggttttatg attatgttca gaaaaaattt accaggtaat
      901 actactgtta cagtatcaaa agctcttcca caaataatca taagtctggt acttgcgaca
      961 tttagttttg ctattgtagg aataataatg gatttaggaa aagttggaat aaatctttca
     1021 agaagcataa tc
//
LOCUS       DAOSAB010000200         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_101413_length_1018_cov_1.854621, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000200 DAOSAB010000000
VERSION     DAOSAB010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101413_length_1018_cov_1.854621"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PK432_03215"
     CDS             complement(<1..872)
                     /locus_tag="PK432_03215"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOK59967.1"
                     /translation="MNDFTTVFEALKGLLPMKYFEDIVEYTNADHGAKKLTALRHLCI
                     LMYAHLKEKKSLRDITSGISADPNLQEYTGTISYSQLSRKNSDRDPEVFEQMFQAIVS
                     QVVLYQGIRDIPSTWGDIKILDATLIRVCLSLFPWAHYRKAVGAVKMHTLIDLVSDCP
                     EKIVITDGKIHDRDKMDSFVTKPGITYIFDRGYTDYGEYDRLCMDDIFFISRLKKNAV
                     MEVVSENQVSRDSNVLSDKEVFLGGFYTRMKNTVRIIEVMDISKGESFFIVTNRFDLS
                     AEEIARKSLEIAAT"
BASE COUNT          295 a          207 c          181 g          335 t
ORIGIN      
        1 atagttgcag ctatctcaag agattttctc gcaatctctt cagcacttaa atcaaagcgg
       61 tttgtaacga taaagaagga ttctccttta gagatatcca taacttcaat tatcctaaca
      121 gtgttcttca tcctagtgta gaagccacct aagaatactt ctttatctga cagaacatta
      181 ctatctctcg aaacttggtt ctctgaaact acttccatca ccgcattttt cttgagcctg
      241 gatatgaaga agatatcatc catacatagc cggtcatatt ctccatagtc tgtatacccg
      301 cggtcaaaaa tgtaagtgat accaggctta gtaacaaatg agtccatttt atctctgtca
      361 tggatcttgc catcggttat cactattttc tcagggcaat cactaactag atctataagg
      421 gtatgcattt taactgcccc aacagctttg cgatagtgag cccatggaaa gaggctaagg
      481 catactctaa ttagagtggc atctagaatt ttaatatcac cccatgttga tggaatatcc
      541 ctaatgcctt ggtatagtac aacttgagaa acaatagcct gaaacatttg ttcaaatacc
      601 tcaggatcgc ggtcactgtt tttacgggat agttgggaat aactaatggt accggtgtac
      661 tcctgcagat taggatccgc gctgatgccg cttgtaatat ctctaagact cttcttttcc
      721 tttaagtggg catacatcaa gatgcataag tgtcttaggg cagtaagctt cttagctcca
      781 tggtcggcat tggtatattc aacaatatct tcaaaatatt tcattggtaa aagacctttt
      841 aatgcttcaa atactgtggt aaaatcattc atgttgatct ctccttgttg attgatgtgg
      901 gtgattggat cccctcactg atattaacaa ggagttttat tatatatcaa caggtaatta
      961 tttgaagcat aggcacttat tatccattga ctttcagtaa atttttttct gcaacact
//
LOCUS       DAOSAB010000201         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1920 NODE_102659_length_1010_cov_1.678534, whole genome
            shotgun sequence.
ACCESSION   DAOSAB010000201 DAOSAB010000000
VERSION     DAOSAB010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863449
            Sequence Read Archive: SRR4236669
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:07:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 645
            CDSs (total)                      :: 630
            Genes (coding)                    :: 623
            CDSs (with protein)               :: 623
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102659_length_1010_cov_1.678534"
                     /isolate="AMR_MDS_1920"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PK432_03220"
     CDS             complement(<1..626)
                     /locus_tag="PK432_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59968.1"
                     /translation="MIKLPLKKSLGRSLLDGNTPIVALDIGTETVKSILFTMNDYGVT
                     VNKVSRIQQQQHAMRSGIITNLDTVLENCKLSINELLTNLKPEEFPRYVMMGIAGEYV
                     QGVSIVVNYEREQHFEKEVTQKEQEKIIKEVKSQIAVTGKDDLSLRTGLKNDDIEILH
                     ITPTGMEIGGMPVNSLVGYKGRNVKLNFYASFAPRTYTEALRKVASSL"
     gene            complement(658..>1010)
                     /locus_tag="PK432_03225"
     CDS             complement(658..>1010)
                     /locus_tag="PK432_03225"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
                     /protein_id="HOK59969.1"
                     /translation="IDGLDVGDTGEIVLIDRKQLSEYGMFIIDINLSANTHRLMGNPQ
                     FVSRGFIYMKKSQELLKEIKTIIYDVHRDWMSISKKNKNYDYKELKAKMEKAASKYIY
                     KKTEREPIIQIVVV"
BASE COUNT          269 a          193 c          137 g          411 t
ORIGIN      
        1 ttaagagaag aagcgacttt tctaagagct tctgtatatg ttcttggagc aaaagatgca
       61 taaaagttaa gctttacatt tcttccttta tatccaacaa gagagtttac aggcattcct
      121 cctatttcca tacctgttgg tgttatatgt aatatctcta tatcatcatt tttaagacct
      181 gttcttaaac ttaggtcatc tttacctgtt actgcaattt gggactttac ctcctttatt
      241 attttttcct gctctttttg tgttacctct ttttcaaaat gctgctctct ttcgtaatta
      301 acaactattg atactccttg tacatactct cctgctatac ccatcattac atatcttggg
      361 aactcttctg gcttaagatt ggtaagtaac tcgttaattg aaagtttgca gttttcaaga
      421 actgtatcta agtttgtaat tattccactc ctcattgcat gttgttgctg ttgtattcgt
      481 gatactttgt ttacagtaac accatagtca ttcatagtaa acagaatgct ttttactgtt
      541 tctgttccta tatcaagtgc gactattgga gtatttccat caagaaggga ccttcctaat
      601 gattttttta acggcagttt tatcatactt tttaattata tcatcaatgc ctattaatta
      661 tacaacaaca atttggataa taggctctct ttcagttttc ttatatatat acttactagc
      721 tgctttctcc attttagctt taagttcctt gtaatcatag tttttgtttt tcttagatat
      781 gctcatccaa tctctatgta catcatatat tatggttttt atctctttaa gaagttcctg
      841 ggactttttc atatatatga agcctctgga tacaaactgg ggattcccca ttaatctatg
      901 agtatttgca ctaaggttga tgtcaattat aaacatacca tactctgaaa gctgttttct
      961 gtctattaga actatctctc ctgtatcacc tacatcaaga ccatctatta
//
