LOCUS       DAOSAI010000001        23976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_1520_length_23976_cov_3.776389, whole genome shotgun sequence.
ACCESSION   DAOSAI010000001 DAOSAI010000000
VERSION     DAOSAI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 23976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23976
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1520_length_23976_cov_3.776389"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..660)
                     /locus_tag="PLM83_00005"
     CDS             complement(154..660)
                     /locus_tag="PLM83_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOK69829.1"
                     /translation="MMIDYYAILGVSQHAGEELIRAAFREKARLWHPDVCHSPEAHER
                     FLVISRAYSTLIDPTRRAQYDRDLARADKTRIRSGFSCSASTSARFSHKLLVGLAGVL
                     LIIASALAIAGTAIPLALPPLIPLLVWLLHQSKLIGLQVIVTYTILASCLLLGLLLLG
                     LGLAYLSA"
     gene            complement(685..1203)
                     /locus_tag="PLM83_00010"
     CDS             complement(685..1203)
                     /locus_tag="PLM83_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOK69830.1"
                     /translation="MIAGEPLDVVRAFEDAYNDHDAKTINSLYAPDAKFQVEDIMAIE
                     GKPALQGKTNYDIELHTHMHINPVSVEENRLRARVMASNEWLLETGIGTAQYEAEFEV
                     ADGMIKSIIARPVPQTKAAIGRVLNDFIEWAMTERPCEAGILMPDGRIVFNAHNARLG
                     LIMLKEWKAKRD"
     gene            1813..2592
                     /locus_tag="PLM83_00015"
     CDS             1813..2592
                     /locus_tag="PLM83_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK69831.1"
                     /translation="MINALGLTKRFGDTLAVDSLDLHVGAGEIFGFLGPNGAGKTTSI
                     NMMIGILTPTAGRVELAGIDVAKDPLKAKAIIGYVPDQPNIYEKLTAWEFLMFVANLH
                     EVERGRAEKKARNLLKMFDLLDRADEQLGGYSHGMKQKVVIASALLHEPKILFMDEPT
                     VGLDPGSARLVKDILRELASEGVTVFMSTHVLEIAERMCDRVGIIQKGRLLAVGNMDD
                     LREVAAAKGAMKHPEASTLEDLFIELTGGSDYAEVSRYLEG"
     gene            2598..4391
                     /locus_tag="PLM83_00020"
     CDS             2598..4391
                     /locus_tag="PLM83_00020"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69832.1"
                     /translation="MDKTFLILSVELRQAFRGTRGESEKSSQGKRIARNALGYLALIS
                     LAIFVGTSVYNIAAALSDTLAAYPDMVRAVWLNLMSGLSLGVFVRLFMTGISVVYQAL
                     YDSGDLKFLLSTPTPVGSVVGAKLTVALVRNLAALVPFMYPIWVGYGAGSRAPFSFYF
                     MSILAILLAALLFTAVTAIFAMIVMRNVPTQKMRQVIMIGSLVVGFMFVLATQVLSAK
                     MTRSNGLDATALAQAAQGWGLGRTWYLPHVWVVKTAFLTTPGFGFSLWESLFPLAVAA
                     IGAFVGAIGLSANAFVTGWSAAGDVETGKSGAMRRRRTRKGTGEKAICCELADDAESE
                     MPDTMIRRHTGQGWGIFAKDLTIIKRQPIIWYSILVAIVASGFYLYNMSYGARSGDVG
                     AIPDIMKDVILFVLVMMSTISSGQIAGMCISMDGESLWLLRSMPIDPGTYFNAKMALA
                     VMPGATVFTVLALAMALIPDIPQYPLYLSLPVGYAAISMLGALEVMSDAMRPNFSLRM
                     SGASGTQNQKDPGKSLISLGISMAGTFVLGAAFAFNNYYSHLGFSEGMSRQAAILVGT
                     IAFATIVVAGHAIARSLALASIKRILRGESN"
     gene            4514..4660
                     /locus_tag="PLM83_00025"
     CDS             4514..4660
                     /locus_tag="PLM83_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69833.1"
                     /translation="MDLSKSFDCVNHDIVMAKVARVVEDRLVLKLIREFFEARVMEDD
                     VCKN"
     gene            complement(5184..5939)
                     /locus_tag="PLM83_00030"
     CDS             complement(5184..5939)
                     /locus_tag="PLM83_00030"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOK69834.1"
                     /translation="MLGYSYLRGAEKVLDEIATTQMGAIEKAASAIANSLSHGGLLYL
                     FGTGHSASVAVEPFHRAGSLVPVRPIVEHAMTLHSSPKKATALERLEGYAGIILDDSG
                     IRPGDSLIVISNSGINSVPVEMALGAKSRGVTVIAITSVRHSKAESSRHPSGKRLFEA
                     ADIVVDNCSIPGDAMLDTCASPIKIGPTSTLAGIAIVNAISVKVIGKLHEMGIEPPVM
                     MSSNIAGGDDFGAKWDEAYADSEAARSLASKRP"
     gene            6511..7944
                     /locus_tag="PLM83_00035"
     CDS             6511..7944
                     /locus_tag="PLM83_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK69835.1"
                     /translation="MISTLDFAKPLVNQALGYLQADPVGNLPKALAIAEKLASQENHK
                     QQVREVGKAILDPDSNWRELAIRLLTETHPNIMKGIGVNFFVNSALVGVPTQYDLAEE
                     LGVQVPYAILMDPTEKCNLRCTGCWAGDYQRVRELPFETMDRVCTEAEELGIYLIVVS
                     GGEPMVAKDKLIRLAEKHPNQLFHPFTNGTLFDEEFVSEMIRLGNIAPAISVEGLEEQ
                     TDARRGKGVYQKIMNTMDMLREAGCVFGFSACYARNNTEIIASDEFIDTMIDKGCSFG
                     WFFTYVPVGSDPNLDLMATPDQRALMYDTVRQWRFTKPIFMVDFWNDGEFALGCIAGG
                     RRYLHINANGDVEPCAFIHYAVDNINEKPLKECLASPLMKAYQKRQPFNDNMLRPCPL
                     IDNPEKLVEIVEESGAHSTQLGEHLVDHRELAEMIQEGYTKHWAPKADEIWNANPHPF
                     FEMQNKYYEEERTEDRKKQKEIQSKQG"
     gene            complement(8294..9406)
                     /gene="mnmA"
                     /locus_tag="PLM83_00040"
     CDS             complement(8294..9406)
                     /gene="mnmA"
                     /locus_tag="PLM83_00040"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOK69836.1"
                     /translation="MGPNINNQTIAVAMSGGVDSSVAAALLVAQGHRVIGITMTTWHW
                     PGPFEGDACGDSFGEEAADSAARVARSLGIDHVVVDLSEEFKKTVVADFVRAYAGGST
                     PNPCVICNQLIKFDLLHHKARELGADMVATGHYARLRQDAVSGRYLLLRPADSRKDQT
                     YMLYRLGQEQLARSVFPLGDLEKVQVRKIAKSIGLEAADRPESQEICFLPPGKYREFL
                     ASNDPSFQTPGPIMNRAGHVIGEHRGIAMYTVGQRKGLSLAADRPLYVLEIRPDSNTI
                     MVGPVERLYSSWLEVESTNFIPFDTLSDKMDVQAKIRYRSPAAKAVIFPAANGRVRVE
                     FNEPQRAITPGQSAVFYDGDLLVGGGIISSNQKADV"
     gene            9724..10941
                     /locus_tag="PLM83_00045"
     CDS             9724..10941
                     /locus_tag="PLM83_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HOK69837.1"
                     /translation="MYTARISISGHLIDSGLLSRLLDAVSDLGGKFEIVETQFGRMKE
                     EESCATIEVGADTCEELDKILDKAKELGATVFDGEPVQLAVVEQDGVCPDEFYTSTNI
                     ETYVNIDGHWVPVDDIEMDCAIVVDAQKPAARCCPLAAVSRGEYVVVGSSGIRVASMA
                     SAKETEVFSFMGSDVSSERPKALVVEQIAREMKEVRARGGKILVVAGPAVIHTGAGPH
                     LARLIDAGYVNVFYAGNAVATHDVEWALLGTSLGVYLKDGTSAPMGHTHHLRAINTIR
                     KAGGLRPAVEKGILTKGVMYSLVTNDVPYILAGSIRDDGPLPDVITDSVKAQDAMRKN
                     LKGVEIALMLSTMLHAIATGNLLPARVKTVCVDINPATVTKLADRGTFQAIGIVSDVE
                     WFLKELAGHLAAN"
     gene            11076..11690
                     /gene="pncA"
                     /locus_tag="PLM83_00050"
     CDS             11076..11690
                     /gene="pncA"
                     /locus_tag="PLM83_00050"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOK69838.1"
                     /translation="MEALLLVDLQNDFLPGGALGVLEGDQVIPVANRVQEYFDIVIAT
                     ADWHPPGHGSFASSHPGCKAGDVIELSGMPQVLWPDHCVQHTEGAEFSPHLNTGKIDK
                     VIYKGMDPEIDSYSGFFDNGRQKATGLETYLRDQKVDTVYVMGLATDYCVKYTALDAV
                     SLGYRTWLIEDGCRGVGVAEGDVERALAEMKAAGVRVVKSGDLG"
     gene            11781..12956
                     /locus_tag="PLM83_00055"
     CDS             11781..12956
                     /locus_tag="PLM83_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOK69839.1"
                     /translation="MKFQGLVIGVPTEIMKGERRVAAMPDTVKKMTAEGAKVLVQKGA
                     GEGSYFHDEEYSAAGAEIVDNVEDLFARADIILKVKEPIFNDAVGKHEVDMMHEGQCI
                     ITFLHPAAPANHQMVQDMAAKGVISLTLDGIPRISRAQTMDALTSMSTVAGYKACLMA
                     ANRLPKFMPMVGTAVGMIKPSTVTVIGTGIAGLQAVATAKRLGAVVNSIDIRPDANEQ
                     AKSLGAKVVDVGVPSEIAIAPGGYAAKLPDEWLEKERETVKPVVIDSDIVITAALIPG
                     KLAPVIVTEEMVKAMRPGSAIVDIAIDQGGNVEITQPGEVIEKYGVSIDGTKNIPGMM
                     PTASTWMFANNIYNYTANLVKDGKIDLNLDDEIIKSSLVTRDGQIVHEGAREAMNMK"
     gene            12972..13256
                     /locus_tag="PLM83_00060"
     CDS             12972..13256
                     /locus_tag="PLM83_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOK69840.1"
                     /translation="MSELSLIILFAIATFVGYKVISQVPSLLHTPLMSGTNALSGVTV
                     LGALSATAAAKAQGASAVGAVLGCVAIIMAMINVVGGFLVTDRMLRMFKK"
     gene            13292..15289
                     /locus_tag="PLM83_00065"
     CDS             13292..15289
                     /locus_tag="PLM83_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOK69841.1"
                     /translation="MDWNEAIFLGLSLADLLGLVFVAGTVWGIRLMSSPATAVRGNLI
                     GAVSMTGAIIVTLVAEDIVSVQILWLGMAVGGALGYILAARVAMIEMPQMVALLNGFG
                     GGSSAIVSLLTLLGPGAIGPFEKVTAILGLLVGAITLSGSLIAAGKLAGKINQRPVIF
                     ENQTFVNAVAIIISVAAGVRAVLAPADAVLWPSVIALVASLAYGVLFTIRVGGADMPI
                     TVSLLNSLSGVAASVSGLAIRNPLLVAIGAVVGASGLILTQIMCRAMNRSLGQVLTGK
                     TTVAHIKGSEAKPVSAPTKAAAGETSAQARLASKAEKPEKADYISIAKAAKKVIVVPG
                     YGMALAQAQGDVKALMDKLEAGGADVKVAIHPVAGRMPGHMNVLLAEVDVPYDKLYEM
                     DQINDEFAETDLVIVVGANDVINPAANTAEGTPIYGMPILNVYQAKHTVIFNYDTKPG
                     YAGVDNPLYEADNVTLVLGDAASTVKEFMAELDASGAAAESQLSPGKVDCLDIARAAK
                     KVIVVPGYGMAIAQAQRDVKALMDKLEAGGADVKVAIHPVAGRMPGHMNVLLAEADVS
                     YDKLYEMDQINDEFAETDLVIVVGANDVINPAANTAEGTPIYGMPILNVYQAKHTVIF
                     NYDTKPGYAGVDNPLYEADNVTLVLGDAASTVKEFMAKLGL"
     gene            complement(15731..17563)
                     /locus_tag="PLM83_00070"
     CDS             complement(15731..17563)
                     /locus_tag="PLM83_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOK69842.1"
                     /translation="MRYRSAAVIVSLAFILIASFNPAFGATANVSTPGLGFEGAEVAI
                     LDSGYTRHYADLKAWTYSEEFIDATWLMSTLARSGISAAVINDHDIEIGALSSYKALI
                     LPATSCMSSLAIDQIEAFATAGGRVFSTYDTSLRDDFWRTRTDYGLSSIMGVRYSSTG
                     TSGNNHIVPAAKSHPIFRGIESDIPVPSTYGLTNKMNPNAIILAKWSTGRSAIVETEY
                     GIYCSENLFARKNLSSPEVVTLITNTIDYLVNTKYKAKFNLGRLDTRATWYRPAAREE
                     QIRADVKRLANANFNLVFVDSFYDGYTIYPSDVGVQNPIYSEFDPLAIAIDEGHKHGI
                     QIHAWLETFCVGFASEKETPPQILQENPDWAAVGLDGRIPSAAESDRCFLSPAHPQVQ
                     EFILDLAWELVTEHAVDGVHFDYLRYPYGRTIPYDYAPTITKLATEDLGFQPRDVRLD
                     IAKWNAWFDWRCNNLTSFMIKLSDAIRSAAPGTLVSAAVYPYPEAILTRMQDWKAWCE
                     KGCLDFVVVFTYTQDLDLFDTMIRAAQSFAGNKTQMIAGIDADSMTHSQAGEGVPALV
                     EAARAAGARGVSIFSANAISENVLTSLKSGPFDPSTRSETPFYP"
     gene            18147..18434
                     /locus_tag="PLM83_00075"
     CDS             18147..18434
                     /locus_tag="PLM83_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69843.1"
                     /translation="MLSDITGDSIYLDVARGVVNSWIDKVLIRRQDGVMDWNWDYGGK
                     VGKLNWTAEEFDKTQGAFVSGFWATRDGHEWVCSVRRLRSAPALPSLLTLF"
     gene            18816..19859
                     /locus_tag="PLM83_00080"
     CDS             18816..19859
                     /locus_tag="PLM83_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOK69844.1"
                     /translation="MNRIRKSSMLLSIVLLLALMMGIGIPMEAAAKPRVAIVFATGGL
                     GDKSFNDSAYDGMKRAEKDLGIEFDKVEPQAVAEYETLLTRFAQTRKYDLIISIGFDQ
                     ADALKVVSARFPNQKFAIIDTAVDAPNVASYVYKEAERGFVVGAIAGLMTTKKGDPRI
                     VSDKQVVGVIGGMDIPLIRANVAGYIAGAKYVNPKVQVKYAYVGDWADPAKCKEMANA
                     MFDEGVDVLWLAAGRSGLGGIKAAEERNRYAIGADSDQGYLAPRNILTNGMKLVDRTV
                     YIAINDVLRGKFKPGVNMLGLKEGALGYSKSLVPADVMSQVDKLAAKVVSGDINVPDE
                     IDKVEPWLKAQGK"
     gene            19961..21475
                     /locus_tag="PLM83_00085"
     CDS             19961..21475
                     /locus_tag="PLM83_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK69845.1"
                     /translation="MYAVRMENITKRFGSVTANEAVDFAAFPGEIHCLLGENGAGKST
                     LMRILFGLCRADSGLICIDDEPVDISGPRDAISLGIGMVHQHFRLAERLTVEENIIAG
                     CEPSKNGLVDYDGARERILEVSQRYGLTVDPTAVVEDISVGQQQRVEIIKALMRGAKV
                     LILDEPTAVLTPPEVEELFVVMRALRDAGTTIIFITHKLKETMEISDRVTVLRDGRTV
                     GVVNTADATPDSLVEMMVGRSLPIHDSHRVPAKDGIFLQLKDVSVAGKKGSRARLDSV
                     NIAVRGGEILGIAGVDGNGQLELEEVATGLQRPSKGQIIVNGQDVTGSDTRSIRDSGV
                     AYIPSDRLKRGMVDSFSIARNSVLGMHHSAHFSHRGVIKEDVVRRHAENLVDVYDVRS
                     ESIDQPIGSLSGGNQQKLVVGRELLRPHSVVVAAQPTRGLDVGATHYIREQILASRNK
                     GKAVLLISAELDEILELSDRIAVIYEGRIVDVRPTNQYTERELGLLMTGQRNGV"
     gene            21479..22585
                     /locus_tag="PLM83_00090"
     CDS             21479..22585
                     /locus_tag="PLM83_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK69846.1"
                     /translation="MDISGDASNKLSKKNAEWFARPGFARFVYSAAAVVLALLIGAVL
                     IGVSGYSVKEAYYNLFMGAFGGVYSLATTIVMATPLLFTGLAVAFAFQCGLFNVGAEG
                     QLYVGALASAVVPLALEGLPPVLRVIIALLAGAGAGAAVGFLPGYLKAKIGAHEVITT
                     IMLNYIATLLTTFLVKTYFRDPGPVDQTVLLPLGSRLPEIIPGTRVSLGIVIGILAVA
                     LLNYVIRRTVLGYEIQAVGQNPHAAEYGGVNVRARMVQAMMISGAVAGLAGSVEAMGT
                     LYRFIPYFSPGYGFTGMAIAVIAGESLWAVIPAAILFGALQSGGQSMQLFAGIPMELM
                     TMVQGLVILFVAAPGMAEMIFRRKSCGKEGGGRA"
     gene            22582..23529
                     /locus_tag="PLM83_00095"
     CDS             22582..23529
                     /locus_tag="PLM83_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK69847.1"
                     /translation="MSGFVDIVFNVNNLAATIRMATPIALAAMGGAFSERSGVVNIGI
                     EGMMLTGAFCGVLGSHLTGNAWLGVLFAIVGGGLVGALLAVFAIRLKADHVVTGVGIN
                     ILASGATTWQMQVIWKRKGASDLVAGLGTWSVPGLCDVPVLGTILGTHSPLVYISFVL
                     IFLGWVLMFKTPLGLRIRMTGEHPEAADTVGINVHAIQYFCVILSGCLAGLGGAYLSL
                     AQLNWFSKDMSAGRGYMALAACIFGGWNPLGGLGASFLFSFTDAIQMRMQTAQISLAV
                     EIIQMIPYILTILILASAVVRSRPPAALGKHYESGHANK"
     gene            23664..>23976
                     /locus_tag="PLM83_00100"
     CDS             23664..>23976
                     /locus_tag="PLM83_00100"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOK69848.1"
                     /translation="MDDLSEKNRKEDRQDVNGANPDWGGGFGLSCADVRHIDASEIAE
                     AVYKLCMKVTCVLPPENIAALERAMEEEKSPQGRQALARILENHRLAGELMQPACQDT
                     GI"
BASE COUNT         5532 a         5717 c         7010 g         5717 t
ORIGIN      
        1 ttttcatgca ggctaaagcg atttcagagg ctatgtaagg tctaaagctc gcaaaagatc
       61 ggagtttatc tccgccctcc gccatgatac aatggccgag ccttaagatg gcaaagagca
      121 tctttgtggg ctcggcctcg tccttttacg cgttcatgcc gaaagatagg cgaggcctaa
      181 ccccagcagt aagaggccga gaagcagaca actggcgaga atggtgtatg tgacgatgac
      241 ttgtaacccg atcagcttcg actggtgcag aagccataca agaaggggta tcagcggtgg
      301 cagggcaagg gggatcgcgg tccctgcaat tgcgagggct gaggcgatta tcaacagcac
      361 gcctgcgagt cctaccaaaa gcttgtgact aaacctggca gatgtggacg cagaacatga
      421 gaagcccgac cgaatccgcg ttttatcagc gcgcgcaaga tccctatcgt actgggcgcg
      481 acgggtgggg tcgataagtg tgctgtatgc ccgggaaata actaaaaagc gttcgtgcgc
      541 ctcagggctg tggcacacgt cgggatgcca cagcctagct ttctcgcgaa aggccgccct
      601 tatcagttct tcaccggcgt gttgagaaac gccgagaata gcgtaatagt cgatcatcat
      661 gcaccaccat catgcgtgtg gggctcaatc gcgctttgcc ttccattctt tgagcatgat
      721 cagccccagt ctggcattgt gcgcattgaa cacgattctg ccatctggca ttagaatgcc
      781 cgcctcacag ggccgctccg tcattgccca ctcgatgaag tcgttcagaa cgcgcccgat
      841 tgcggcctta gtctgaggaa cgggtctggc tatgatcgac ttgatcatac cgtcagccac
      901 ctcgaactcc gcctcatact gggctgtccc gattcctgtt tcgaggagcc attcattaga
      961 cgccattact cgggcgcgca gcctgttttc ctctacagag acaggattaa tgtgcatgtg
     1021 agtatgcagc tcgatatcgt aattagtctt tccttgaagg gcgggtttgc cctctatggc
     1081 catgatgtct tcgacttgga attttgcgtc cggggcgtat agagagttta tggttttcgc
     1141 atcatgatcg ttgtaggcgt cctcaaatgc tcggacaacg tcgagaggtt caccggcaat
     1201 cacattccaa cacctcgctc atcttgatca gattgtcgaa cgtcgctagc gcaggatttt
     1261 ctgagcgcgc gatggatcat ggatgatagc ctagaggatt gaagataggc ttgatcctcc
     1321 ttcacgtgta tctcctgagg atttgtcaga cagccgggtt gcatattgcc tattctagat
     1381 tcgcccacgt acacctgcaa actattccat caaggcgttt gcctgccact tagctgcgtc
     1441 catcataagc gaatacttcc ggtcttgccg cttctgtggt gttggaacgt tcaattttgg
     1501 agatggctgc acggatcttg aatggtaggc gcttcgaggc gtaccgcatg tcggggccga
     1561 atatgacttt aaagctccca aatatcatct taccatgtgc cgtgctagag aaggtgatgt
     1621 ataattgtat gagggtggct gttccctcat gcttcgcgtc gtcaaggcag aagacgggtt
     1681 tgagatggca aaaaagaacg cagacggggc catgaagatc tattcgaatt ctagatcttg
     1741 acgacctctg atagggatat gccttttggc actgtgattt tgaaggtggc tgcgaacagg
     1801 agggattctt tgatgatcaa tgcactcggg cttaccaaga ggttcggcga cacgcttgcc
     1861 gttgacagcc ttgatcttca tgtaggcgca ggcgagatat tcggctttct cggtcccaat
     1921 ggagccggta agaccacttc tatcaatatg atgataggga ttctcactcc taccgcgggt
     1981 cgcgtcgagc tcgcagggat agatgtggca aaggatcctc tcaaggccaa ggcgattatt
     2041 ggttacgtgc cagaccagcc caatatctac gagaaactta cagcctggga gttcttgatg
     2101 ttcgtggcca atctccacga agttgagaga ggacgggccg aaaaaaaggc taggaacctt
     2161 ttgaagatgt ttgatcttct ggatcgggct gatgagcaac tgggtggata ctcgcatggg
     2221 atgaagcaaa aggtcgtcat agccagcgcg ctcttgcatg agcctaagat actgtttatg
     2281 gacgaaccta ctgtaggact tgatccaggg agcgctaggc tggtaaagga tatcctccgc
     2341 gagcttgctt cggagggcgt cacggttttc atgagcacac atgtactaga gatcgcagag
     2401 cggatgtgcg atcgggtggg tattattcaa aaggggagat tgctggctgt gggcaacatg
     2461 gacgatctac gtgaggtcgc ggcggccaaa ggcgccatga agcatccgga ggcttccacg
     2521 ttggaagacc ttttcataga actaactggc ggttccgatt atgcagaagt ctccagatac
     2581 ttggagggct agacgacatg gacaagacgt ttctcatcct atcagttgaa cttcgacaag
     2641 cgtttcgagg aacccggggt gagagtgaaa aatcgagtca gggcaagcgc atagcgagaa
     2701 atgccctggg ctatttggct cttatcagtt tggccatttt cgtaggtacc agtgtgtaca
     2761 atatcgctgc ggccttgtcc gatacgcttg ccgcttatcc agacatggtg cgggcagtat
     2821 ggttgaacct gatgtcaggt ttatcactag gagttttcgt caggctcttc atgacaggta
     2881 tctccgtggt ctatcaagct ttgtatgatt cgggcgatct caaattcctg ttgagcaccc
     2941 ccacacctgt gggttcagtg gttggggcga agttgacggt ggcccttgtg aggaacctgg
     3001 cggcgcttgt cccattcatg tacccgatat gggttggata tggtgccggt agtcgcgcac
     3061 cgttctcttt ctacttcatg tccatcctgg cgatcctcct cgctgcactg ctgtttaccg
     3121 cggtgacggc cattttcgca atgatagtaa tgaggaacgt tccaactcag aagatgaggc
     3181 aagtcatcat gatcggctcg cttgttgtgg gatttatgtt cgtcctcgcc acgcaagttc
     3241 tcagcgccaa gatgacccgt tccaatggcc tcgatgccac agccttggcg caggcggccc
     3301 agggctgggg actgggcagg acatggtatt tgccacatgt ctgggtggtt aaaacggcct
     3361 ttctcactac gccgggattt ggattttcac tctgggaatc gctttttcct ctggctgttg
     3421 ctgcgatagg ggcatttgta ggcgccatag gcttgtcggc caatgctttt gtgactggtt
     3481 ggtcagctgc aggcgatgtg gagactggca agtcaggcgc catgcgtcgt cgacgaacgc
     3541 gtaaagggac cggagagaaa gcaatctgtt gtgagctggc tgacgacgcc gaatcagaaa
     3601 tgcccgatac gatgattcga agacataccg ggcagggttg ggggatcttt gccaaggact
     3661 tgaccatcat aaagagacag cccattatat ggtatagcat tcttgtagcc atagtagcat
     3721 ctggatttta cctctataac atgtcctacg gggcgcgttc gggcgatgtg ggagccattc
     3781 ccgatataat gaaggacgtg attctttttg tcttagtcat gatgtcgacg atttcttcag
     3841 ggcagatagc cggcatgtgc atcagtatgg atggggagag cctctggctg cttcgatcca
     3901 tgcccatcga ccctgggacg tacttcaacg ctaaaatggc gctggcggtc atgcccgggg
     3961 ccacagtctt caccgtgctc gcgctggcta tggctcttat ccccgacatt cctcaatatc
     4021 cgctctacct gtcgcttcct gtgggatacg ccgccatatc catgcttggc gcgctggagg
     4081 tcatgtctga tgcaatgagg cccaatttca gcctcagaat gtcgggggca tcagggacgc
     4141 aaaaccagaa agaccctggc aagtcgctca tatccttggg aatcagtatg gcagggacat
     4201 tcgtcttggg tgcggctttt gcattcaata actactattc gcatctcggc ttttctgagg
     4261 gtatgagtag acaagcggca atccttgttg gcacgatcgc ttttgcaact atagtcgtcg
     4321 caggacatgc gattgcgcga agtctggctt tagccagcat caagcgtata ttgagaggcg
     4381 aatcaaactg agcaaaaacc gctttgtgct gggcacaggc gtacgttgtc cccatcggtt
     4441 tcatgggatg ctgtaagcga tctagaaggt cgtaaaacag agggggaagg tggggaccga
     4501 ttggggattt cctatggacc tatccaaatc cttcgactgc gtcaatcatg atatcgtcat
     4561 ggccaaggtg gctagggtgg tcgaagatag gctggtgctc aagcttatcc gggagttttt
     4621 cgaggctaga gtcatggaag acgacgtgtg taagaactga gtagggcgag gcacaagtcg
     4681 gtccgaggag tcctctgttc gccaacatca tgctgaacga cctaggatga gaactcgaga
     4741 agagagggca tagattcact aaaagtcggc gcgccggaga acgggtgatg gcgagcgttc
     4801 gacgttttgt ggaggggaaa tctgaagttc aagctccaat tgtggaaatc aggccgcaac
     4861 cacgacccgg gagcggtcta aagagcggat ttggaggctc atcggcctca gcgagaggtc
     4921 gagtatcgac agaggccttt gcgagctcag tctctacctg tgtgggtgga cgggatactg
     4981 ccggatcgcg cggacaccta gggtatacca gaggctagac taatggatgc agcgatgcac
     5041 gcggcttggc aaggtcatcc aaagccgaag tcttctcgga caccggtatt ttgcaaggca
     5101 acgcgggttt gaatactggg gtgttgccgc gttctttcct actgtggata ggcttttagc
     5161 gaagtggaag cgcaacttgg cgcttagggc cgtttgctcg caagcgacct tgctgcttcg
     5221 gagtctgcat aggcctcatc ccattttgct ccgaaatcgt ccccgcctgc tatgttcgag
     5281 ctcatcatca caggcggttc aatacccatt tcatgcagct tgccaatgac cttgacagat
     5341 attgcgttga caatcgcgat cccagctaat gttgatgtcg ggccgatctt tatcggggat
     5401 gcgcacgtat cgagcattgc atctcctgga atgctgcaat tgtccaccac gatgtctgct
     5461 gcctcaaaca acctcttgcc gctaggatgg cgggaagact cggctttact gtgtcttact
     5521 gacgtgatag ctattacggt gaccccccga gacttggcgc caagcgccat ttcgaccgga
     5581 accgagttga tgccggaatt ggatatgaca ataagcgaat cgcctgggcg gatgcccgag
     5641 tcgtccagta tgatgccggc atatccctcc aggcgttcca gggcggtggc ctttttaggg
     5701 gagctgtgca acgtcatggc gtgttcgact atgggtctga caggcaccaa actgcctgcc
     5761 cggtggaacg gttctacggc gactgacgct gaatgtccgg tgccaaaaag atatagcagg
     5821 cctccgtggc ttagcgaatt tgcaattgcg gaagccgcct tctcgatcgc gcccatttga
     5881 gtcgtggcga tttcgtcgag caccttttcg gcgccacgta aatatgaata gccaagcact
     5941 gcctagccca cctccggaca atccgtcctg ctcgcgaatt tgctacagaa tcgagaattc
     6001 tgaacttaat tcgattttcc ttcatggtat ttgtgacaaa tcgagtctga aggacaaaga
     6061 tctgtgcggc aatgggaata cttcagaagg tatatgtata tcaatgtaga ataaatctac
     6121 aaatcagagt ggtgtgtccg tatgtcgaac tgacggatag gtgagtgctt cagggggcca
     6181 gcacgtgaca gaactggagc gagggtggtc gcctgaaatt ggcaggaagc gaaagggacg
     6241 tggcatggct atatttcggc gcctatcgct gtagccataa aggcacctcg tagattctcg
     6301 gatttggtgt acatatcggc gcattgcggc gtatctcgcg ttcgcgttta tttgaaaact
     6361 ctccttcaaa gggatagaat ggcatgtgca tacattcata gtaatgaact gtaatcttga
     6421 catctgagga tggaggcgta tttgaagggt gtgatgttat cttcatcgga gtgatgcagg
     6481 aaaaaaatcc agtgattgga ggggattgat gtgatatcta ctttggactt tgctaagcct
     6541 ttagtaaacc aggcgcttgg atatctacag gcagaccccg taggcaatct gccgaaggcg
     6601 cttgcgattg cggaaaaact ggcgtcccag gagaatcaca agcaacaggt ccgagaagtc
     6661 ggtaaggcta ttcttgaccc agatagcaac tggcgtgagc ttgctattcg cttattaacg
     6721 gagactcatc ccaatataat gaagggcatt ggagttaatt tcttcgtcaa ctctgcgctc
     6781 gttggagtgc cgacacagta tgatctcgca gaagaactgg gcgtacaggt tccttacgcg
     6841 atactcatgg accccacgga aaagtgtaat ctcaggtgta cagggtgctg ggctggcgac
     6901 taccagcgcg tccgtgaact cccgtttgag accatggata gggtatgcac cgaagctgag
     6961 gaattaggca tttatcttat agtagtatcc ggcggggaac ccatggttgc caaggataaa
     7021 ctgatccgtc tggctgaaaa gcatccgaat cagctgtttc atccgttcac caatggcacg
     7081 ctgttcgacg aggaattcgt ctccgaaatg atcagactgg gcaacattgc tcccgctatt
     7141 tccgtggaag gtctcgagga gcagacagac gcaaggcgag gaaagggcgt ctatcagaag
     7201 ataatgaaca ccatggatat gctgcgcgaa gccggatgcg tattcggatt ctccgcatgc
     7261 tatgcccgca acaacacaga gataatcgcc agcgatgagt tcatagatac catgatcgat
     7321 aagggttgct cgtttggatg gttcttcacg tacgttcctg ttggatccga ccctaacctc
     7381 gatctgatgg caactcccga tcagcgtgct ctgatgtatg acacagtacg tcagtggcgt
     7441 ttcacgaagc cgatcttcat ggttgacttc tggaacgacg gcgagttcgc gctcggatgc
     7501 atagcgggtg gcaggcgcta tctgcatatc aacgcaaatg gcgacgttga gccgtgtgca
     7561 ttcattcact atgccgttga taacataaac gagaagccgc ttaaggagtg cctggcttcg
     7621 ccactgatga aggcttacca gaagcggcag ccgttcaacg acaacatgct gaggccgtgc
     7681 ccgctcatcg acaatcctga gaagcttgtc gagatcgtgg aagagtcggg tgcgcattcc
     7741 acacagcttg gagagcatct tgtggatcac agagagcttg cagagatgat ccaggaagga
     7801 tacaccaagc attgggctcc gaaggctgat gaaatatgga atgcgaatcc gcatccgttt
     7861 ttcgagatgc agaataagta ctacgaggaa gagcggactg aggataggaa gaagcaaaaa
     7921 gaaatccaga gcaaacaggg ctaaacagta acccaacaga atagcattga aagcccgcgt
     7981 ctgagacgcg ggcttcttgc cgcgttacgt ctgtctaatg aggcctctcg cgaagtcggc
     8041 gccctgtttt ctgcatggtt ttccgtagcg ttactccttt ccgagcagaa ccggtttggt
     8101 caacgtttga agaatacagt agcgcgctcc agcgtctaat cccgtcttga cctcgtaaaa
     8161 ctcttgtatt tgaggagact tgctgcggct ctgaataacg acatatgctt catgcacaat
     8221 gagggctata cggttcggca gcccgattct gcaagggaga gtccgagcag tccagcggga
     8281 atgggacatg ccctcagaca tcagctttct gattgcttga aatgatcccg ccgcccacaa
     8341 gcaaatcccc gtcatagaaa acagcagatt gtccaggagt aattgcccgc tgaggctcgt
     8401 taaactctac gcgcactctc ccattggctg cagggaatat tactgctttg gctgccggcg
     8461 atctatatct tatttttgcc tgcacgtcca tcttgtcgct tagtgtgtcg aaggggatga
     8521 aattagtgct ctcaacctcg agccagctgc tgtagaggcg ttcgacgggg ccaaccatta
     8581 ttgtgttcga gtcgggtcgt atctccaata cgtaaagtgg ccgatcggct gcgagactca
     8641 gtcccttcct ttggccaacg gtatacatgg ctatgccacg gtgctcccct ataacatggc
     8701 ccgctctgtt catgatggga ccgggagtct ggaaggaagg atcatttgat gccaagaact
     8761 cccgatactt gcccggggga agaaagcata tctcctggct ttccggtcgg tctgccgctt
     8821 caaggccgat ggacttggct attttgcgaa cctggacctt ttcgagatcg ccgagaggaa
     8881 agactgaacg cgccagctgt tcctgcccga gtcgatagag catgtatgtc tggtctttgc
     8941 gagaatcggc cgggcggaga agcaggtatc ttccggaaac cgcatcctgt cgcagccttg
     9001 cgtagtgtcc ggtagcaacc atgtctgcgc cgagttctct ggccttgtga tgcaataggt
     9061 cgaacttgat caactgattg catatgacgc acgggttcgg ggttgatccg ccggcatagg
     9121 cgcggacgaa gtccgcaaca acagtcttct tgaattcctc ggacaggtct acaacgacat
     9181 gatcgatgcc tagcgatcga gcgacccgag ctgcggaatc tgccgcctct tcgccgaagc
     9241 tgtccccgca cgcatctcct tcgaaagggc cgggccaatg ccaggttgtc atcgtgattc
     9301 ctatcaccct gtgtccttgg gctacgagga gcgcggcggc gactgacgag tctacgcccc
     9361 cgctcatggc aactgctatt gtctgattgt tgatattcgg gcccatgggc gcccctcctc
     9421 ttaccgcgtg agtccgctgt gcttcacaat tgttctgttg tgcttgcggt tgcgtcaacc
     9481 atgattacta tagcccactt tcggttctgc gtggaagatt cgctgtaggg aaccttgttc
     9541 ctgatggtca tatctgatac tattggctag ggatatacag gcaatgatat cttaatatgg
     9601 ttatacatta tacatagcta ttgcataatc cttcggcaaa gcaccccatt attcacgagg
     9661 ccatgaagca ggcagagtcc agcatgttta gaacaaaagc ttccggatag gagtgatgcg
     9721 ccggtgtata ctgcgaggat ttcgatttcg ggccatctca ttgattcggg tttgctgtcg
     9781 cggcttcttg atgccgtctc cgatcttggg ggaaagtttg agatcgtcga gactcagttc
     9841 gggcggatga aggaagagga gagctgcgcg actatcgagg tgggtgctga cacgtgcgag
     9901 gagctagaca aaatccttga caaggcgaag gaactggggg ccaccgtctt cgacggcgaa
     9961 cccgttcagc ttgccgtcgt cgagcaggac ggtgtttgtc ctgatgagtt ctatactagc
    10021 acaaatattg agacttacgt caatatcgat gggcactggg ttcctgtgga cgatatagag
    10081 atggactgcg cgattgttgt tgatgctcaa aaaccggcag ctagatgttg ccctctagcc
    10141 gcagtttccc gtggtgagta cgtggtggtt ggaagcagcg ggataagagt agcctcaatg
    10201 gcctcggcca aagagactga ggtcttctcc ttcatgggca gtgatgtatc gagcgagcgg
    10261 cctaaggctc tggttgtgga acagatagcc cgggagatga aagaggtgcg ggcccgtggc
    10321 ggaaagatcc tagtggtggc aggtcctgca gtgattcaca caggcgccgg tccacatctt
    10381 gccaggctaa ttgatgcggg ttacgtaaat gtcttctatg ccgggaatgc agtggctacg
    10441 cacgatgttg aatgggcttt gctcggtact tcccttgggg tttatctcaa ggacgggaca
    10501 tccgccccta tgggccatac gcatcacctc agagcgatca acactattcg aaaggcgggc
    10561 gggttgaggc ctgcggtgga gaaggggata ctgaccaagg gggtaatgta ctccctagtt
    10621 actaatgatg ttccgtacat tttggcaggt tccatcagag atgacggccc gttgccggac
    10681 gtgataaccg attctgtcaa agctcaggac gccatgagga agaacttgaa gggtgtcgaa
    10741 attgccctga tgctttcgac catgttgcat gcaatcgcaa cagggaatct tctcccggcc
    10801 cgtgttaaga cggtatgtgt tgacataaat cctgctacag tcaccaaact tgccgaccgg
    10861 ggtactttcc aggctattgg gatagtcagt gacgtggagt ggttcctcaa ggaattggcc
    10921 gggcatcttg cagcgaatta gcctttagtg cactgcttgt gatggtgtaa cttggctgcg
    10981 catgacaggt ccgaaaaccc atatacatgt catgctgttg cgacaggata tttggctctg
    11041 atgtagaaaa ctgcacaatg tgggggtgcc ttggtatgga ggccttattg cttgtcgatc
    11101 tgcaaaatga tttcttgccg ggcggtgctc tgggggtgct agagggcgat caggtcattc
    11161 ctgtcgccaa tagagtacag gaatacttcg acattgttat agcgacagcc gattggcatc
    11221 cgccgggtca tgggagtttt gcctcttcac atccgggatg caaggcaggc gatgttattg
    11281 agctttccgg catgccacag gttttatggc ctgaccattg tgtgcagcat actgaaggag
    11341 ctgaattctc tcctcatctg aacactggca agatcgataa ggtgatctat aagggaatgg
    11401 atccagaaat agacagttac agcgggttct tcgataacgg aagacaaaaa gctactggtc
    11461 tcgaaacgta tcttcgggat cagaaggtag acacggtgta cgttatgggc ttggctactg
    11521 actactgtgt caaatacact gctcttgacg ccgtatcctt gggctacaga acctggctca
    11581 ttgaggatgg gtgcaggggc gtgggagttg ccgaagggga cgtggagcgc gcgttggctg
    11641 agatgaaggc agcaggtgtt cgcgtagtga aatcgggaga tctaggttag ccgcgggagt
    11701 gacctgggga taatgttctg caaacgctgg agcgttatct catgatatac cataaatcat
    11761 ggaacaggga gtggctacag atgaaattcc aaggtttggt tatcggagtc cccaccgaga
    11821 tcatgaaggg ggagcggcga gtagccgcga tgcccgacac tgtcaagaag atgacagctg
    11881 aaggcgcaaa ggtactggtt cagaagggcg ctggagaagg ttcctatttc cacgatgaag
    11941 agtacagtgc agccggggct gagatcgtcg acaatgtaga agacctcttt gctagggccg
    12001 acatcattct caaggtgaaa gagccgattt tcaacgatgc cgtaggcaag catgaagtgg
    12061 atatgatgca cgaaggccaa tgcatcatca ccttcctcca tcctgcagct ccagccaatc
    12121 accaaatggt tcaggacatg gccgcaaaag gcgttatatc cctcaccctt gacggaatac
    12181 ctaggatctc aagggctcag accatggatg ccctcacctc aatgagcacg gttgccggtt
    12241 acaaggcttg cctgatggca gcaaacaggc ttcccaagtt catgcccatg gtgggaacgg
    12301 cagttggcat gatcaagccg tctacggtta ccgttatcgg aacgggcatc gcgggtctgc
    12361 aagcggttgc cacggctaag aggttgggtg ctgtggtcaa ctctatcgat atcaggcccg
    12421 acgccaatga acaggcaaag agccttggag cgaaggttgt cgatgtgggc gttccatctg
    12481 agatcgccat tgcgcctggc ggatatgcgg ccaagctgcc cgacgagtgg ctcgaaaagg
    12541 agcgggagac agtcaagccc gtagtaattg attccgacat agtcatcact gcagccctga
    12601 tcccgggcaa gctcgcaccc gtgatcgtaa ctgaagaaat ggtcaaggct atgaggcctg
    12661 gctcggcgat tgttgacatt gcgatcgacc agggcggaaa cgtggagatc acccagcctg
    12721 gcgaggtcat agagaaatac ggcgtcagta tcgatggaac aaagaatatt cctggaatga
    12781 tgcccacggc ctccacatgg atgttcgcaa acaatatcta caactacacc gcaaacctcg
    12841 tcaaagacgg caagattgac ctgaatcttg acgatgagat catcaagtct tccttggtca
    12901 cccgtgacgg ccagatcgtc catgaaggcg ctcgtgaagc aatgaacatg aaatagggag
    12961 ttgtgacgtc cgtgtctgaa ctgtcactta taatcctctt tgccatcgct acatttgttg
    13021 gctacaaggt gataagccaa gttccaagcc tgcttcacac gcccctcatg tcgggcacaa
    13081 atgctctttc cggggtgact gtgttaggcg cactcagcgc aacagcggca gccaaagccc
    13141 agggagcttc ggctgtaggt gcagtgctcg gatgcgtagc gataattatg gcgatgatta
    13201 acgtggtcgg gggcttcttg gtcaccgaca ggatgctaag gatgttcaaa aagtagggag
    13261 ggttcagcgg cactgaccgc tgtatgatct gatggattgg aacgaagcaa tctttctcgg
    13321 tcttagcctg gcagacttgc tagggctggt cttcgtggca gggactgtct ggggcatcag
    13381 gctgatgagc tcccccgcta ccgccgttag aggcaacctc ataggcgcgg tgagcatgac
    13441 aggtgcaatc atagtcacgt tggttgcaga ggatattgtc agcgtacaga ttctctggtt
    13501 agggatggca gttggcggtg cgctaggcta tatccttgca gcaagggtgg ccatgatcga
    13561 aatgcctcag atggtcgccc tgctaaatgg gtttggcgga ggcagctctg ccattgtttc
    13621 actccttacg ttgctgggcc cgggggcaat tgggccattc gaaaaagtaa ccgctatcct
    13681 cgggcttctt gtcggcgcga taacgctttc cggaagcctt atagccgcgg ggaaacttgc
    13741 aggcaagata aatcaaaggc cagtcatctt cgaaaatcag acatttgtaa atgctgtcgc
    13801 catcatcatt tcggtggcgg cgggagttcg ggctgttctg gctccagcag atgcggtcct
    13861 gtggccgagc gtgatcgcac ttgtcgcgtc gcttgcgtac ggggtgctct ttacgatacg
    13921 cgttggcggg gccgatatgc cgattactgt gtcgctgctt aactcgcttt caggtgtggc
    13981 ggcatcagtt tcggggcttg ctattcgaaa tccccttctt gtggcaatag gagcagtagt
    14041 aggcgcttca ggcctcatcc ttacacagat aatgtgcaga gcgatgaaca ggagccttgg
    14101 ccaggtgctt acgggcaaga ccactgtggc gcatatcaag gggagtgagg caaaaccggt
    14161 ttcagcccct acaaaggctg cagctgggga gacctctgct caagcaagac tggcgtcgaa
    14221 ggcagaaaag cccgaaaagg ctgactacat ctctattgca aaggcggcta agaaggtaat
    14281 tgtggttccc gggtatggca tggcgctagc tcaagcgcag ggcgacgtga aggccctcat
    14341 ggataaactg gaggccggcg gggcagacgt caaagtggca atccacccag tggcgggccg
    14401 gatgccgggg cacatgaatg tgcttttggc agaagtcgat gttccatacg acaagctata
    14461 cgagatggac cagatcaacg acgagtttgc cgagacggat ctggtgatcg tggtcggagc
    14521 gaacgacgtt atcaacccgg cggcaaatac cgcggaagga acgcccatct acgggatgcc
    14581 cattctcaac gtataccagg caaagcacac tgtaatattc aactacgaca ccaagcccgg
    14641 atacgcaggg gtcgataacc cgctgtatga ggcggacaat gtgacgctgg tccttgggga
    14701 tgcggcgagc acggtaaagg agttcatggc ggagctcgat gcaagcgggg ctgctgctga
    14761 gtcgcaactg tcgccgggca aagttgattg tcttgacata gccagggccg caaagaaggt
    14821 catcgtagtg cccgggtatg gcatggcgat agctcaggcg cagcgcgacg tgaaggccct
    14881 catggataaa ttggaggccg gcggcgcaga tgtcaaggtg gcgatccacc cagtggcggg
    14941 ccggatgccg gggcacatga atgtgctttt ggcggaagct gacgtctcat acgacaagct
    15001 atacgagatg gaccagatca acgacgagtt tgccgagacg gatctggtga tcgtggtcgg
    15061 agcgaacgac gttatcaacc cggcggcaaa taccgcggaa ggaacgccca tctacgggat
    15121 gcccattctc aacgtatacc aggcaaagca cactgtaata ttcaactacg acaccaagcc
    15181 cggatacgca ggggtcgata acccgctgta tgaggcggac aatgtgacgc tggtccttgg
    15241 ggatgcggcg agcacagtaa aggagttcat ggcgaagctc ggactttagc cgaactccat
    15301 ggcgggatag tgtttccaca ttagatagct gcaaaccctt cgcccggcgg aagccggagc
    15361 ggagggtttc atttctcgct gctatgacat tattgttccc gcaggggaac cgatcggctt
    15421 aggtaaagga atgtgttagg atcccaaccc ccgccttatg aagcaggcag tcaagcccta
    15481 atgctagttt cgtcagtggc gccgaagacc tagggttggc agactcatgt ccgttttccc
    15541 tacgccctca tctaggaaat tgaccagcat gattccgagc cgtattttcg gacgcttggc
    15601 gggccaggac agactcaaat ggctgaaatg gtcgtcagtt tgaacagtag agctggagcg
    15661 aaggctattg gcgatgacaa ttgggcgtgc aataacacgc tatgtgagaa acacggcctg
    15721 gctgggcaag ctatggatag aatggggttt ctgacctggt ggaggggtca aatggccctg
    15781 atttaagtga ggtcagaaca ttttcagata ttgcgtttgc gctgaagatc gatacgcctc
    15841 tggcgcccgc cgcgcgagct gcttctacga gggcaggcac gccttcgccc gcttgggaat
    15901 gggtcatcga atctgcatca atccccgcga tcatttgcgt cttgttcccg gcgaaggatt
    15961 gggccgcccg tatcatcgtg tcgaacagat cgaggtcttg cgtgtaggtg aagacgacta
    16021 caaaatcgag gcaccctttc tcacaccaag ctttccagtc ctgcatcctt gtgaggattg
    16081 cttcaggata gggatagacg gcggcggaga ccagagttcc tggagcagca gacctaattg
    16141 catcggagag ctttatcatg aaactggtaa gattgttgca ccgccagtcg aaccaagcgt
    16201 tccatttcgc tatgtcgagc cttacgtcac ggggttgaaa gcccaggtct tccgtagcca
    16261 gtttcgtgat ggtgggtgca tagtcgtacg gaatggttcg gccgtacgga tagcggaggt
    16321 agtcaaagtg aacaccgtcg acggcgtgtt ctgtgaccaa ttcccacgca aggtcgagta
    16381 tgaactcctg aacttggggg tgagctgggg acagaaagca cctatctgat tcggctgctg
    16441 aaggaatgcg gccatcaagc ccgacggccg cccaatccgg gttttcctga aggatctgcg
    16501 gcggcgtttc cttctctgac gcgaatccga cacagaaggt ctcaagccag gcgtggatct
    16561 gaataccgtg tttgtggcct tcgtctatgg ctattgcaag ggggtcaaat tccgaataaa
    16621 tggggttttg cacgccgaca tcgcttgggt agattgtgta gccatcatag aaagagtcca
    16681 caaagacgag gttgaaattg gcgttggcga gccgcttcac atccgcccgt atctgttctt
    16741 ctctggccgc aggtcgatac cacgttgctc tggtgtctag ccgccctagg ttgaacttgg
    16801 ccttgtattt cgtgttgacg aggtaatcga tggtattggt tattaatgtg actacctcgg
    16861 gagatgaaag gttcttgcga gcaaaaagat tctctgaaca ataaatgcca tattcggttt
    16921 cgactatcgc agatcggcct gttgaccact tggccaggat gatcgcgttc ggattcatct
    16981 tattggtgag cccataagtg cttggcacag gtatatctga ttcaatgcct cgaaaaatag
    17041 ggtgcgattt ggcagcgggg actatgtggt tgttccctga agtgccagtc gatgaatatc
    17101 gtacgcccat aattgagctg agtccgtagt cagtacgggt ccgccaaaaa tcgtcgcgaa
    17161 gggatgtgtc gtatgtagaa aatactcggc ctcccgccgt cgcaaaagcc tctatttggt
    17221 cgattgcaag agaagacatg cagctggttg caggcaggat caaagctttg tatgacgaca
    17281 gagcgcctat ttcgatatca tggtcattga ttacggctgc cgaaatgccc gaccgtgcca
    17341 atgtactcat tagccatgta gcgtcaatga actcctcgct atatgtccac gctttaagat
    17401 ccgcgtaatg cctcgtatat cccgaatcga ggatcgcgac ctctgcgcct tcgaacccca
    17461 acccgggagt tgaaacgtta gcggtagcgc caaaagcagg attgaagctt gcgatcaata
    17521 tgaatgccaa ggatacaata accgcggcag agcgatatct caaagtgaat tcacctcatt
    17581 ttggccttgg aaagtcccaa tttaaatcat aattcttcgt aaggggcagg ttttcctcca
    17641 gcaatacgca ttcgcacatg gcttccatat cttaatgggg tttactgaga ttggctgaaa
    17701 ctaccacagt tgtgccaagg cgcggacttt gcctttaagc ctactaaccc aattggagaa
    17761 attcgggcca ttgggtgtaa acttgagttt ggggtgatta gcggtggcag ctggctgccg
    17821 aagcggcgcg aagtgccgaa cacatggagg agactgacgc gattgtgtcg aatggttgat
    17881 tgctgtccgg gagcaaatct agggaaacgc tcgtcttcgc tcagctgtag agcgtttacg
    17941 cctcagagat tgggttgatg cgcatcgaag tatttgtcgg gggtgcaatc tcagcaattc
    18001 tgattccagt gttccggtat tagggatatc caattcgccc cgagccatgc atatgtaggg
    18061 gtgttgcttc catcacatga gatctggtta gtcaatgata ttgacccgat gccacgatgc
    18121 cgttttatgg gtgttgacag ctgggcgtgc tttcggatat taccggagac agcatctatc
    18181 tggatgtcgc gcgcggggtg gtcaattcat ggattgacaa ggtgctcatt cggaggcaag
    18241 atggtgttat ggactggaac tgggattatg gtggcaaggt cggtaaactg aactggaccg
    18301 ccgaggagtt tgacaagaca caaggggcat ttgtctccgg attttgggcg acaagagatg
    18361 ggcacgaatg ggtttgcagt gtgcggaggc tccgctctgc gccggcgttg ccaagcctct
    18421 taacgctctt ttagaatttg tgaggctgcc gagggttgtg gtgcagacat tgattgcatc
    18481 ctctggggat ctcgtcgaca gtctgctatc gctcaaacag gcatccaggt atatttcacc
    18541 aaacccgctg cctatggcac gcatcgggtc gtagccgcca cggcagacgc ttacaaggta
    18601 accgggcggt acaagtgctt ggtgtatgct attgccaggg ccgattggct aataagccag
    18661 acggaaaccg agtttcaagg atacggaagc aatgtatgat ctgtgatatg gactcttgaa
    18721 gttatggaag cagtcatcag tgctgacgag gctatgagcg cggacaagta gcgctcaaat
    18781 caaacagttg caaatctcag gagggaattc tagggatgaa cagaatccga aagtcgtcaa
    18841 tgctcctaag tatcgttttg ctcttggcat taatgatggg aataggcatc cccatggagg
    18901 ctgcggccaa gcctcgtgtg gctattgttt ttgctacagg cggattgggt gacaagtcgt
    18961 ttaatgactc tgcttacgac ggcatgaagc gagcagaaaa agacttggga atcgaattcg
    19021 ataaagttga acctcaagct gtagctgagt atgagacatt gttgacgcgt tttgcgcaga
    19081 ctcgcaagta tgatctgatc atatcgatcg gcttcgatca ggctgatgct ctgaaagttg
    19141 tatcagcgag gttcccaaat cagaaattcg ccattatcga cacagcagtg gacgctccca
    19201 atgtagcgtc gtatgtatac aaggaggccg agcggggatt tgtggttggc gccatagcag
    19261 gtctgatgac cacgaaaaaa ggcgacccgc gcattgtttc tgacaaacag gtggtcggag
    19321 tcatcggtgg catggacatc ccgcttatcc gggccaacgt cgcgggctac attgccggtg
    19381 ccaagtatgt gaaccccaag gttcaggtga agtacgcata cgtaggcgat tgggccgatc
    19441 cggccaagtg caaagagatg gcaaacgcca tgtttgacga gggcgtcgat gttctgtggc
    19501 ttgcagccgg gcgttctggc cttggtggca tcaaagcggc agaggagcgg aatcgctacg
    19561 caataggcgc tgactcggat cagggctatc ttgctccccg caatatactg accaatggaa
    19621 tgaaactcgt ggaccgaaca gtgtatatag ccatcaatga cgttctgcgt ggcaagttca
    19681 agcctggagt caatatgctc ggactcaaag aaggggcact tggttactcc aagagcctgg
    19741 ttcctgcaga tgtcatgtcc caggttgaca agcttgcggc caaagttgta tccggcgata
    19801 tcaatgtgcc tgatgagatc gacaaggttg agccctggct caaggcccaa ggcaagtagc
    19861 aagctgtcag cgggttgaat taatatgtaa gcgcaatggg cggggggata atctccccct
    19921 gccttgccag gtttaactcg gaaaaggggt tcaccttacg atgtatgctg ttcgaatgga
    19981 gaacataacg aaacgctttg gaagcgttac cgccaatgaa gcagttgatt ttgctgcgtt
    20041 tcccggcgag atccattgcc ttctgggcga gaacggggcc ggaaaaagca cgctcatgag
    20101 gatcttgttt gggctttgcc gcgcggattc aggcctcatc tgcatagacg acgagccggt
    20161 agacatatcc ggtcctcgtg atgcaatttc gttggggata ggcatggtgc atcagcattt
    20221 caggctcgct gaacgtctta ctgtagagga aaacataatt gccggatgtg aaccgagcaa
    20281 aaatggtttg gttgattatg atggggcacg cgaacgcatc ctggaggttt cgcagcgtta
    20341 tggcctcaca gttgatccaa ccgctgtggt cgaggatatc tccgtcggcc aacagcagcg
    20401 ggtggagata atcaaggctc tgatgagagg agcaaaggtc cttatcctgg acgaaccgac
    20461 tgcggtcctc actccgcctg aggtggagga gttgttcgtt gtgatgcggg ctttgaggga
    20521 tgcggggacg acgataatat tcatcacgca caagctcaag gagactatgg agatttccga
    20581 cagagtgaca gtgctccgcg atggcaggac agttggtgta gtgaatacgg ctgatgctac
    20641 accagacagc cttgtagaga tgatggtggg gcgcagcctt cccatacatg acagccatcg
    20701 cgtgccggct aaagacggga ttttcctcca actgaaggat gtgtcggtag ccggaaaaaa
    20761 gggcagccgg gcaaggctcg acagtgtgaa catcgctgtg agaggcggcg agatactggg
    20821 catagcgggg gtcgacggca atggccagct ggaactggag gaagtagcaa ctgggttgca
    20881 gcggcctagc aagggtcaga tcatcgtcaa tggtcaggat gtgactggct ccgatactag
    20941 gagcatcagg gatagcggtg ttgcctacat cccatctgac cgattgaagc ggggcatggt
    21001 ggactccttc agcattgcga ggaattccgt gctcggaatg catcatagtg cccatttcag
    21061 tcatcgcggg gtaataaaag aggacgttgt gcgacggcat gcggagaatc ttgttgatgt
    21121 ttatgacgtg cgatctgaat cgatcgatca gcccatcggt tcgctgtcag gggggaacca
    21181 gcagaaattg gtggttggaa gggagctgct tcggcctcac agtgtcgtgg ttgccgccca
    21241 acctactcgc ggattggatg ttggagcaac ccactatata agggagcaga tacttgccag
    21301 ccgcaacaag ggcaaagcag ttctcctcat ttccgcggag cttgacgaga ttcttgagct
    21361 gtctgacagg atagcggtca tatacgaagg ccgaatagtg gacgtacgtc ctacaaacca
    21421 gtataccgag agagagctag gccttctcat gacagggcag aggaacgggg tgtgaccgat
    21481 ggacatttca ggtgatgcgt caaacaagtt gtcgaaaaag aacgcagaat ggtttgcccg
    21541 tcccgggttt gccagattcg tgtattcagc agctgcagtc gtgcttgcgc tgctaatagg
    21601 ggctgtcttg ataggtgtct caggctatag tgtgaaagag gcatattaca acctcttcat
    21661 gggagcgttt ggaggcgtat acagcctcgc aactaccata gtaatggcga ctccgctcct
    21721 gttcaccgga cttgcagtag catttgcatt tcagtgcggc ttgttcaacg tgggcgctga
    21781 aggccaactc tatgtgggcg ctctggcatc tgccgtagtc cctctggctc ttgaggggtt
    21841 gccgcctgtg ctgagggtga tcatagctct cctggcgggg gcaggcgcag gggcagcggt
    21901 tggctttctg cccggatatc tcaaagcaaa gataggggcc catgaagtca tcaccacaat
    21961 catgcttaat tacattgcca cattactcac cacttttctg gtgaaaacgt acttcagaga
    22021 cccgggcccg gttgatcaga cagtcctgtt gccccttggt tctagattgc ctgagatcat
    22081 acctggaacc agagtctcgc tgggcatagt aataggaatt cttgctgttg cgcttctcaa
    22141 ttacgtaata cgcaggactg tgcttggata tgagatacag gctgttgggc agaatcctca
    22201 cgctgccgag tacggcgggg tgaatgtcag agcaagaatg gttcaagcta tgatgatcag
    22261 cggggctgta gcaggccttg ccggctcagt ggaggccatg gggactctgt atcggttcat
    22321 accatacttt tcacccggat acggctttac tgggatggcg atagcggtga ttgccggcga
    22381 gagcctctgg gctgtgattc ccgcggcgat ccttttcgga gcgcttcaat caggcggcca
    22441 gtcgatgcag ctttttgcgg ggatacccat ggagttgatg acgatggtgc aaggactggt
    22501 gatactgttt gtagctgcgc caggcatggc ggaaatgata tttcggcgga agagttgtgg
    22561 caaggaaggc ggtggccggg catgagcgga tttgtcgaca tagtgttcaa tgtgaacaac
    22621 ctggcggcta ccataaggat ggcaacgcca atagccttgg ctgcgatggg gggcgccttc
    22681 tctgagagat ccggagtggt gaatatcggg attgaaggca tgatgctcac aggcgcgttc
    22741 tgtggtgtgc tcgggtccca ccttacggga aacgcatggc tgggagtgtt gtttgcgatt
    22801 gtcggcggcg gacttgtcgg cgccctcctt gcggtatttg ccatccggct caaggcggat
    22861 cacgttgtaa ccggggtggg aataaacatc ctggcctcgg gcgccacaac atggcagatg
    22921 caggtgatat ggaagcgcaa aggcgcatcc gatctcgttg ctggtctcgg aacctggtca
    22981 gtgcctgggc tgtgcgatgt cccggttctc gggacgattc tcggtactca ttcgcctctg
    23041 gtgtacatca gttttgtgct gatttttcta ggttgggttt tgatgttcaa gacgcctttg
    23101 gggcttcgca tcaggatgac aggcgagcac ccggaggcag ccgatacagt gggcatcaac
    23161 gtccatgcaa tacagtattt ttgcgtgatt ctcagtggat gcttggcagg gctgggagga
    23221 gcatatctct cccttgctca gctcaattgg tttagcaaag acatgtcggc tgggcgcggt
    23281 tacatggccc tggcggcatg catattcggc ggttggaatc ccctcggggg cttgggcgcc
    23341 agcttcctgt tttcatttac agacgccata cagatgcgaa tgcagacagc tcagatatcg
    23401 ctggctgtcg agataatcca gatgatccct tacatcttga ctattctgat tcttgccagt
    23461 gcagtagttc gctctaggcc tccggcggct cttggcaagc actacgagtc tgggcatgca
    23521 aacaagtgac tttgaagtca tgagttagct gtgtcatggt gctttgttgc gcctgttgcg
    23581 ggctgactgc ggctttattg ccgggtcagc ccgtcgctat ttaccaccat ctttttggaa
    23641 atgggcgccg gggcgccgaa ggaatggatg atttgagcga gaagaatagg aaagaagaca
    23701 ggcaagacgt gaatggagcg aaccctgatt ggggaggagg attcggtttg agttgcgcag
    23761 acgtcagaca tatcgatgca tctgaaattg cggaggcagt atacaagctg tgcatgaagg
    23821 ttacatgcgt gctccctccg gagaatatcg ctgctttgga gagggctatg gaggaagaaa
    23881 aatcgcctca aggcaggcag gcgcttgcca gaatactcga aaaccacaga ctggccggcg
    23941 agctaatgca acctgcttgt caggacactg gcattg
//
LOCUS       DAOSAI010000002        23500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_1563_length_23500_cov_3.638686, whole genome shotgun sequence.
ACCESSION   DAOSAI010000002 DAOSAI010000000
VERSION     DAOSAI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 23500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23500
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1563_length_23500_cov_3.638686"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLM83_00105"
     CDS             complement(<1..516)
                     /locus_tag="PLM83_00105"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HOK69849.1"
                     /translation="MRNRCDIVGIDVGGTNTDAVLVRDEDVIAAAKAPTNPDNLLEGA
                     QIAYRQVVEAHDGTSPIELHLSTTLSTNAIIEGQGDPTVVLATPGPGMSFDDMGLGFP
                     VHALSGYIDHRGREVAPVDVSAAMSTLKAACDDGAKALAIVGKFSHRNPAHELQIEQE
                     VIAEFPQYSPAT"
     gene            728..1402
                     /locus_tag="PLM83_00110"
     CDS             728..1402
                     /locus_tag="PLM83_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOK69850.1"
                     /translation="MQKLYSGKTKDVYLADDGNVLLYFKDSVTGVGDKIDSGANEVIG
                     EVAGKGHSSFSLTLMFFDLLHKAGIPTHFISVGPEDNTMLVKRARSLNLEVICRKKAW
                     GSFVRRYGAYVCEGQPLPSLVEFTLKDDDRGDPLITEDALVALNIASRQDIDYMKETA
                     RRATDLISGYLADRGLELIDIKYEFGVIDGKTMIIDEVSGDSMRVVKDGKVLLQNELY
                     EALLGR"
     gene            complement(1587..1796)
                     /locus_tag="PLM83_00115"
     CDS             complement(1587..1796)
                     /locus_tag="PLM83_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69851.1"
                     /translation="MGSSDTAKSLFFVRVIGGWITAVGSFIVAIAVTTEFSQMYPPCA
                     PQVPVPCANSKEGALKDVANAANWQ"
     gene            complement(2168..2356)
                     /locus_tag="PLM83_00120"
     CDS             complement(2168..2356)
                     /locus_tag="PLM83_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69852.1"
                     /translation="MKSNSCKSTLALLRVIGGWILAVGSFIVAVAVTLEFFQDDCSKH
                     DPDPSGRYVIEVKRAGTD"
     gene            2692..3138
                     /locus_tag="PLM83_00125"
     CDS             2692..3138
                     /locus_tag="PLM83_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK69853.1"
                     /translation="MRQTFLAPAVRDSGRLGWDDMDIAISPNNPVPMYQQIVDQVRAQ
                     ILSGELKPGDPLPSIRKLAADLLASVITTKRAYADLEAEGLIVTRPGLGTFVADLSLE
                     DIERLKAREVIGHLANAVEAGRSLGLEDSEIAELLQNVLDGRVNYG"
     gene            3131..3862
                     /locus_tag="PLM83_00130"
     CDS             3131..3862
                     /locus_tag="PLM83_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK69854.1"
                     /translation="MDDAVLQLCKISKAYPGFELKDVSFELPRGYIMGLVGPNGAGKT
                     TIIRIMMNLARADSGQAAIFGLDNVAHETEVKRRIGYVPENHNFYDDMTAGWMARFVR
                     QYYPTWDQNYFDALAARFEIDLGKRAGALSKGNRAKLSLALAMAHRPELLILDEPTSG
                     LDPVVRRELLGELLDFMGDGERSVLFSTHITSDLDRVADYITIIVDGRIHDSDEKDAL
                     LEKARKDEPTASIEDIVILAARGEK"
     gene            3864..4532
                     /locus_tag="PLM83_00135"
     CDS             3864..4532
                     /locus_tag="PLM83_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOK69855.1"
                     /translation="MTNLIWKDLYVNRSMIFIAIGLFGGLFVFGALTGSEEIAAYSLG
                     IMNTFSPMMVPFCALNSAANEEKNRTLALLRSMPISPRTIVASKFISSLLAGILWSVI
                     NMVAASWGTRLFPGAGGEFPPSSLTGLYMMALTLPIAAVTLIAFFKWNSSAARTAIMA
                     LWVALYVIPTAFRPAGMSMTEFYLKIMSPTPSMISVVLVACLGVYVVGMLVAQAVFAR
                     RELA"
     gene            4607..5281
                     /locus_tag="PLM83_00140"
     CDS             4607..5281
                     /locus_tag="PLM83_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOK69856.1"
                     /translation="MISLVWKDLYTSRLMLLLPIGLFGGLLVFGALTGNDEIASLYFS
                     SLANTYSMLMVPGCAFANASNEEKNRTLSLLRSMPISPQTIVASKFLAPLFAGVLWSL
                     ISVAALLLGARLFPKAAGIALRSTPFLGPYLVALTLPTAAMILIVSFAWNANVAGIAF
                     VVLSAALYMISAIFRPAGMSIMGFYLKIMSPTPSMVSIAFAVSLAIYFLGMLVAQVIF
                     VHRELG"
     gene            5310..7460
                     /locus_tag="PLM83_00145"
     CDS             5310..7460
                     /locus_tag="PLM83_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOK69857.1"
                     /translation="MATVYEHIVNPGSIVVVGASDDVSKPGGRVAKNIVDHGYAGNLW
                     AVNPKHDDVLGLATFKSVGDLPGTPELAIIAIPARGVRGVLEELAAVGTRAVIILSAG
                     FGEVSEEGKREELALVEIAERHGMTLIGPNCMGVLTPCYAGVFAGPIPELEPGCVDII
                     SGSGATADFLAELAIERGLLLSSVFTVGNAAQVGVEDILGMMDEHFGSGSAKVKALYM
                     ESVKKPQKLLRYARSLISKGCSIVGIKAGATGAGKRAAASHTGAMASDDTAVQALFEK
                     AGIIRVSSRSELLDVASVLLMAGPIDGRNVCVLTDAGGPGVLLADELARQGLKVPLLR
                     EATQKRLGEVLWPGSSALNPVDCLSTRTPSQIQQAVDVISEEERDRMDVMPLIMGDPG
                     MGGIAEMYDVIARAMDESPIPVIPVFPSPITSALALEDFKARGKSYFVDEVQLGRSLG
                     RVINRPRLDEPVETLPGYCREAISRALQGDEAHGSAHADNTRTALAPQTAAALLRAAG
                     IPIPGQAVAVGVEDLDGICSDLGFPVVMKVVGPLHKTDVGGVALNVASIEDARDTWRR
                     LMSIDGATGVLVQPMIEGTELIMGATREEGFGALVMFGLGGVFTEVLKDVRFALAPLG
                     MEECKRMIDSIRGRRLLDGARGGRAVNVGAAADILARVGLLVSDFPEIAELDLNPVKG
                     YGSELYAIDARIIVERRPDEGGALARLRSRHHAR"
     gene            7489..7647
                     /locus_tag="PLM83_00150"
     CDS             7489..7647
                     /locus_tag="PLM83_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69858.1"
                     /translation="MGANANFSSGEAVVERTGDAWAMAVKLDGVVVEYESWEYEGRRR
                     NEDARWMA"
     gene            7644..7889
                     /locus_tag="PLM83_00155"
     CDS             7644..7889
                     /locus_tag="PLM83_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK69859.1"
                     /translation="MTIGFAGGEIVGIEGPNASEKSTLLRTMCGIHLPISGEVLVYGK
                     RPDIFDSDFKRRVGYVSSRRTSSFALHCELYGMDKYE"
     gene            8251..8691
                     /locus_tag="PLM83_00160"
     CDS             8251..8691
                     /locus_tag="PLM83_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69860.1"
                     /translation="MSESLLTGMLAPPHPIALLAARSFSGALVSLASIVPATLLVASG
                     CSRFFCGCLLSTWPIRRLPLRPGWPSEEWECRFCGLCCVGGAWRALTDGRMNVRPRAL
                     IQARLRAYRISPAGFWVYVLSVGLYQLMGFAFVVATTSRLISVL"
     gene            complement(8790..9038)
                     /locus_tag="PLM83_00165"
     CDS             complement(8790..9038)
                     /locus_tag="PLM83_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69861.1"
                     /translation="MNPGIKCHGISVNPASTRQAGALATTARGITQVKMMRIASGQKR
                     IGYCPNCTRSIARLIQAERPMLYRTSEAAITKRLYTED"
     gene            complement(9285..10460)
                     /locus_tag="PLM83_00170"
     CDS             complement(9285..10460)
                     /locus_tag="PLM83_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOK69862.1"
                     /translation="MSEEQQTRRRTPAQLRRSALIGVVFSVLGLYVVLQFTGGRTSWK
                     EFAQIDPAMLAIACGLVVVTWLFDSLRMRALIRSLGGDLDILTGMRISIMGAFVSNVT
                     PFDSGGEPMQAYLLTAKGLNAGQSTAVIAVKTICNALARFTLGIAASIWLFGFADFWS
                     IPKAMYVLLAIGIILYFVVFAFSLYLIFHPEKINVIVVPVVRNKFTLKFFKPETLDAV
                     LDRIDHELREFRNALQEFMENKKSALWKVMWLSYAWWITITIVPAVILVGLGITPKFT
                     HIMGITLIFYLAAAYAPTPGSSGAAELGFSLLFSSVVPHGLIGLFVTVWRAFTYYLNL
                     IVGGVLMAVSMIKKNMPSEKQGRSDEIPQPQSEARARESDTISTPSSGAAHTIPPAS"
     gene            complement(10776..11723)
                     /gene="arcC"
                     /locus_tag="PLM83_00175"
     CDS             complement(10776..11723)
                     /gene="arcC"
                     /locus_tag="PLM83_00175"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOK69863.1"
                     /translation="MDNAKTIVIALGGNAILQPGQNGTVDEQMENVDSTAEQVAELIA
                     QGHRVVITHGNGPQVGALLIQQEAGRDRVPPMPLDVCGAESQGQIGYMLQQSIGKMLA
                     RRSIPRPVATVITQMVVDPNDPAFANPTKPVGPFYSAYYAEQRMKETGEKWIEDAGRG
                     WRRVVPSPDPIRIVEQDAILSLVRTGAIVIANGGGGIPVIEKGGFYKGVEAVIDKDLG
                     GECLARDVGADVLLILTDVAHVALHYKTPHQVDLHTVQLDDLIRYQQEGHFNAGSMGP
                     KVEACRRFVERGGEAAIIAQLDKAVEAIHGNAGTRIVKQ"
     gene            complement(11790..12977)
                     /gene="nagA"
                     /locus_tag="PLM83_00180"
     CDS             complement(11790..12977)
                     /gene="nagA"
                     /locus_tag="PLM83_00180"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOK69864.1"
                     /translation="MAHKTLITGGPVIAPTSVIANGAVLIEDSTILNVGSAASLGKET
                     VDATIDAKGGIIAPGFINLHVHGGGGADAMDSSFDSLNCMSKFEASHGVVGFLPTIMS
                     SPLEQMTGACKAAAEAIDADRLGSRCTQGARILGINVEGPFLNSIRKGAQPEEGIMAP
                     NISVLEKLLDAGGGHIMAMTVAPEIPGAIDIIHALASQGILVSAGHSDAAYADMERGV
                     AAGIRHVTHTYNGMRGLHHREPGVVGAALTIDDLTCELIIDGVHVHPIAASIVVRMKG
                     AAHVVLVTDSMRAAGLPDGDYTLATQHVIVKEGQARLESGELAGSTLAMDVAVGNMVR
                     LVGVPLPDAIAMASSTPARQLGLDDRKGRLQRGMDADITILDANTYRATTTISMGKVI
                     FNA"
     gene            13326..14420
                     /locus_tag="PLM83_00185"
     CDS             13326..14420
                     /locus_tag="PLM83_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK69865.1"
                     /translation="MSKLRVGVIGTGMAFERLHYPAYERLSDVYEIGALCDQDAGKLA
                     DWGQRLRLGAESLYTDWRKMVARDDLDVIDIMVPIELNYAITERVARELAGKRKAIIC
                     EKPLAGTFKEALSARELAQRYSVLIMIAENYRYSEEMNIIRDLVVSRRVGDPVYFIYN
                     RTVDFPSDMWKDDFAAREWRQYPEFPGGAIADMAVHDLAAMRHIFGAIDRLQAFGRPQ
                     DAEFSPYSVIVVNMLFASGMIGQFSFFCSGKEMQRPLVGFRIFGTEGMIYLEDRNSGV
                     INVAYNDRRSEQIPYDPQKAYYNELLNLANALRGKEPVSVPPEMEYGDLKTVRDILKS
                     IQEQKIVTVDKTADYLPDYGQPGREGLWLQ"
     gene            complement(14617..14850)
                     /locus_tag="PLM83_00190"
     CDS             complement(14617..14850)
                     /locus_tag="PLM83_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK69866.1"
                     /translation="MTEKKWKAVSTKPRTVTIDKAAEVLAVSTTTIQKMMRDGRLHGI
                     KRGRDWRILRSDLEPFVADAGSHKSAPSNKTER"
     gene            15017..15667
                     /locus_tag="PLM83_00195"
     CDS             15017..15667
                     /locus_tag="PLM83_00195"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HOK69867.1"
                     /translation="MFKARHLPLLLLALIFIMSIFVYRVLPDRVPMHMNWKGEIDRYG
                     SKAEGAFFFPVVILIVHLIMSLVFRAYINSIDADARSVAADRLGIFKSLLILLFAAMY
                     ADFVMVALGRPSSMYEFLGIPVALLFYYTGVLMKDTPRNGIMGVRTKWSMADDEVWVK
                     THHMASTVYRVSALFPLLSLAWPNLFWLWLAVPLILAPTVMIFASRRYYMELVGRK"
     gene            15923..16297
                     /locus_tag="PLM83_00200"
     CDS             15923..16297
                     /locus_tag="PLM83_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69868.1"
                     /translation="MQRRLGICGPKTSSLERHIRLLEEAISPSTPREAVEKWAETRNW
                     ALQYAVIYPELREVARCLVVFDSSTSTGPTVARISVERDKNRARLSRHMSEERWHVVK
                     TSVKPESDCQLKRLVKDLIEEK"
     gene            complement(16511..17917)
                     /locus_tag="PLM83_00205"
     CDS             complement(16511..17917)
                     /locus_tag="PLM83_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOK69869.1"
                     /translation="MKKGMATIGTVLCSIIIVVLALTLVSTTWANSGKPGADSAAGQD
                     ALGQNALAKYVIYINWDACAYRYYEWANSPGQPGTPNLNYLASRGALFTNARNGIPGI
                     TNPMQTSIVTGTWPAVHGNTYRYYDPVENLVKETGRHNDAETIAEVVEKSGLPCASVQ
                     QFMLQERGTYTDDPYHLYIQPGAGWLKRVAEAEKILNLKPVFGMNGKMITPAERPTFM
                     AIYADDLDAAGHNASPGYGFSVARDYDSWKAKMTKLMIDMDEGLGNLIDALKKLGIFE
                     ETAIILTADHGMTPYWGPSSLQDLIKTVESLGYKCEFLGKNERASKNTDIVLVSAGLS
                     VQFYFRNAISDAQYKALIDTLASKPYVGGYFTADDLKKEGAHPSLGSLMIWPEPPNHF
                     HTSGKLIGLGGQHDSGEESSRHVFMLISGAGVKQGVVIDSPVQIIDAAPTMSRLMGLR
                     QPANATGRVLYEALVVDE"
     gene            complement(18065..19177)
                     /locus_tag="PLM83_00210"
     CDS             complement(18065..19177)
                     /locus_tag="PLM83_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOK69870.1"
                     /translation="MKLKRTADSALAKKLNRDVVIDCIRKYGTLSRADIARLTMLSPS
                     TVSSIVAELVESGIVREQKGTAASSGGRRPIMLDLDASASCAAIIDVQVSRVLYGVID
                     LAGNPVWTARDKLDLTSPEDTYHQIFQIVAKTLARLPRSGGQAAGIGVSIPGIVDTTT
                     GTVIKSTRLRLKELPLAARLQQEFSLPSYVERDVRAAAWGERSYGLARSADDFVYVMV
                     CEGGLGAGLILGGKPYAGARMQAGEIGHMTVDIHGKQCSCGNRGCLVTLTYPAIDEVR
                     RNNESDSIVDCAAIGIVNLMNLLDPELVVLGGDIGQEPQFVSQVETVVRDRMVLTPRK
                     NVRLVPSQLGDMGIALGLAGLVFDKFYWSRVFEDVG"
     gene            19620..20486
                     /locus_tag="PLM83_00215"
     CDS             19620..20486
                     /locus_tag="PLM83_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69871.1"
                     /translation="MSNKKTGTIVATAALAAIMLLASIGVSASAADIFAGWSEPMLWE
                     HSGGDTERMPYLAGNDLYFAKDYDIYWSTFDETTGKWSEPEPVPGPINTAANEACPCV
                     VGNGKVLYFSRYDPITDYDFYRSEWDEEKAEWGEPELIEELSTDGQDWAIHVNEDETI
                     AYVISKIPYDEATVVGGRDIWKSVKVDGVWQTPVNLGEPLNSNGEEWSIFVDYMGRFW
                     IDGNRADSLGNFDIYVAENEKATPINLGAPINTDVAEREPTINEKFFFFAANKREGGH
                     GGWDLWYMVRLP"
     gene            20586..21665
                     /locus_tag="PLM83_00220"
     CDS             20586..21665
                     /locus_tag="PLM83_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK69872.1"
                     /translation="MEIDLIQTWIVKEGYWVHTVAIWGAGTMGRVHAKRYAMTGEAQL
                     KYIIDNDLEKATRLAQPFDAVATVDPGPAVRDPQVDVIDVCLPTYLHRESVELAAGHG
                     KHVFCEKPIALTLDDANAMISTCKSNGVKLGIAHVVRYFPEYEMARDMIASGRLGRVG
                     VVRLSRTGPFPGGWNDWYADPQLSKGLFVDLSIHDFDFLNWCFGPVERICARKVATSR
                     PLEYGLATLRMESGVIAHVEGSWAHPRPFSYKFEISGSEGMIECDSAKTKPLTLLTLA
                     QPSSDCSKNEKAREGGPEAGLGVATSESPLRERDPYLREIQDFLAYVRGERLPRVLPE
                     DAVAALEVSLAATRSADLGEAVKVR"
     gene            21701..22675
                     /locus_tag="PLM83_00225"
     CDS             21701..22675
                     /locus_tag="PLM83_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK69873.1"
                     /translation="MSFAHMHAYSYARAVCSLPDAQLVGVADDDEARGKAAAEQYGID
                     YFNDIEQLLDSGVDAVIVCSENARHRDHVVAAARAGAHVLCEKPIAADVCDGAEMIEA
                     CRAAGVVLATAFPVRHVPAVARTKQLVEEGKIGKVLAVKATNHGRMPGGWFIEPDLSG
                     GGAVIDHTVHVADLLRWILNDEFKSVYAEIDTRFYDFDIDDCGTLCLEMENGVFATLD
                     PSWSRPRTYPTWGDVTMEIIGTDGVISLNAFAQCLVEASDSQGAVVYRGWGDNSDLAL
                     VADFVSAAREGRRPAADGVDGLRALEVALAAYESSRLGEVVALDKLDC"
     gene            22755..>23500
                     /locus_tag="PLM83_00230"
     CDS             22755..>23500
                     /locus_tag="PLM83_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK69874.1"
                     /translation="MTKNMSRYGALLLAVVLLVGLSCNVAAQKKVTIVHWQHHSPARL
                     ELVQTFAEEFMKQNPNVKIEIESVPESDYFQKLLPALAAGSGPDTFQIPTGQVPAYAA
                     SGVIQQIDPRVATARQIENDFIAPAIKRLKYDGKYYGFPVDTQTIVLFYNPKLFKEAG
                     LDPARPPQTWDELIKYAQAIHKRDAKGVTTQMGVATGGYGPVLVSLMIQNGAPLWDNK
                     TQLPDFNSPQAIEALKFATDMVTTYKVEDP"
BASE COUNT         5108 a         6023 c         6846 g         5523 t
ORIGIN      
        1 agttgcagga gagtattgcg gaaattcggc tataacttcc tgttctatct ggagctcgtg
       61 ggcggggttg cggtgagaaa acttgcccac aatggccagt gcctttgcgc cgtcatcgca
      121 cgcagctttc agtgtggaca tggcggcaga gacgtccacg ggtgccactt cgcgtcctct
      181 gtgatcgata tacccggaaa gcgcgtggac agggaagccg agccccatat cgtcaaagga
      241 cattcccggg cccggcgtgg ccaggacgac agtgggatct ccttgtccct ctatgatggc
      301 gttggtggac agcgtggtac tgaggtgcag ctcaatcggg ctagtgccgt cgtgcgcctc
      361 caccacctgc ctatatgcga tttgtgcgcc ctccaggaga ttatcgggat tagtgggcgc
      421 ttttgcggct gcaatcacat cttcatccct taccaggaca gcgtctgtgt tggtgcctcc
      481 cacgtcaatg cctacaatgt cgcaacgatt cctcaaattg cccactccca tgaaagactc
      541 agtctctact ttacttctat tatcttcttc aaagccgcct gatttgcctg cccgggagaa
      601 aagtggggtg gagcatatgg cgtcatgggc gagcgcaggg tagaataaga cttatgaatc
      661 gtggtatgca gcatctgcag gcactgatag aatattgaca tgctggacag catggggagg
      721 aatgaatatg cagaagcttt acagcggcaa gaccaaggac gtataccttg ctgatgacgg
      781 caatgttctg ctttatttca aggacagcgt tacaggtgta ggggataaga tagattccgg
      841 cgccaacgaa gtcataggcg aagtggcggg aaaaggacac tcgtccttca gtttgacgct
      901 gatgtttttc gatctacttc ataaggcggg aatacctacg cacttcatta gcgtggggcc
      961 ggaagacaac acgatgctgg tgaagcgagc ccggtccttg aatctcgagg taatctgcag
     1021 gaagaaggca tggggaagct ttgtccggcg ctacggggcg tatgtttgcg aagggcaacc
     1081 gcttccgtct cttgtggagt tcacgttgaa ggacgatgat cgcggggacc ccctcataac
     1141 tgaggatgcc ctagtcgctc tcaacatagc ctccaggcaa gatatagact acatgaagga
     1201 gacagcccgc agggcgacag atctgatctc ggggtatttg gccgaccgcg gcttggagct
     1261 gattgacatc aaatacgaat tcggcgtcat cgacggaaag accatgatta tagatgaagt
     1321 ttccggtgac agcatgcggg ttgtaaaaga cggaaaggtc ttgctccaga acgagctgta
     1381 tgaggctctc cttggacgat agcccatggc cgtggacagt cgagcggggc cggttggagg
     1441 ccgattcggc gaaggctgga tcaggagcta caggcagggc aattcgcgca tacatctact
     1501 ggcagatgag tgcagtgata tgtctggaat ccactaacat gaaatgtcat gtagagtgga
     1561 ttcgcccatg ggcccccgcc cgactgctat tgccaattag cggcatttgc gacatccttc
     1621 agtgcacctt ccttggaatt ggcgcaggga acaggtacct ggggggcaca cggaggatac
     1681 atctgggaaa actcggtcgt aactgcgatt gcaactatga atgagccgac tgccgttatc
     1741 cagccgccta tgacgcgcac gaaaaaaagg gattttgctg tatccgatga ccccatatga
     1801 gagtcctcct cgagagctct tttgctctgt ttgttagggg cagatatgtt tggcgcagga
     1861 aggaatctgg tttactttgc tgtccccaaa gactgttttc gaggcggcag ccagcttatc
     1921 cgtgccgcca tttgggatga gctgaaatcc caaggttttt gacgcgccca gtctcagctt
     1981 ccttacctat ttgacctgcc ttcatctcgt ctcgattagt gccaaacacc agtatcaatg
     2041 aatgggcttc cagaatgtgg cagtataatc ccttcgccct tcattcagta tgggccttgt
     2101 cccggcacac ttcgaatgtg ccgactgcac tatttccccg gtggaaaagt gggctagtgg
     2161 cgtagggcta atcagtgcca gcgcgcttga cttcaatcac ataccgtcct gaaggatcag
     2221 gatcatgctt cgaacagtca tcttggaaga attccagcgt tacggcgaca gccacgatga
     2281 acgagccaac ggcgagaatc cagccgccta tcactctcag gagcgcgagt gtagacttgc
     2341 agctgtttga cttcatgtaa agtggcctcc ccccaatgct ttcgccaaga ctgccggcgc
     2401 tgtcgccttc accttgcatg taccttatac gcaagccatg gctcaagtgt gttcgccttt
     2461 cggcccttca gactctctac cgcgggtgga gcccggaaga tgagtggcgt cggaacggct
     2521 cggaatatct ttcaagtgaa tccatcaaga tgcaggtctg cgtattctcg ccgcaaatat
     2581 cggtgttgac ctgggaaccg gcggcgtata tagtgtgtat agacgatata cacgtatttg
     2641 cccggtcggc gtgcaggtga tgcgcaggag agccatagtt gcattcgggt gatgcggcag
     2701 acgtttctgg cgcctgcagt gcgcgattcg ggacggttag gttgggatga catggatatc
     2761 gccatttcgc cgaacaatcc agtgcccatg taccagcaga tagttgacca ggttagggcc
     2821 cagattctct caggcgagct caagccggga gatccccttc cgtccatcag gaagctcgct
     2881 gcggatctgc ttgccagcgt gatcacgaca aaacgagcct atgcagacct ggaggcggaa
     2941 ggattgatcg tgacaaggcc gggcctgggg actttcgtcg ctgatctttc tcttgaggat
     3001 attgaacggc tcaaggccag agaggtcatt gggcatctgg cgaacgcggt cgaggcaggc
     3061 cgcagtcttg gacttgaaga cagtgaaatt gcggaattgc tccagaacgt gctggatggg
     3121 agggtcaact atggatgatg cagtgcttca gctctgcaaa atctcaaagg catacccagg
     3181 ctttgagctt aaggatgtct cttttgaact gccgcgcggt tacatcatgg gcctggttgg
     3241 gccgaatgga gcggggaaaa cgaccatcat caggatcatg atgaaccttg cgcgcgctga
     3301 ttccgggcaa gcagccatct tcgggctcga caatgtagcc cacgagaccg aggtgaagag
     3361 gcgcataggc tatgttcctg aaaaccacaa cttctacgac gacatgactg caggttggat
     3421 ggcgcggttc gtgcgccagt attaccccac gtgggatcag aactacttcg acgcgcttgc
     3481 ggccaggttc gaaattgatc tgggcaagag ggcaggggca ctctccaagg ggaaccgggc
     3541 caagctttcc ctggcgttgg ccatggcgca caggcccgag ctgctcatcc tggatgagcc
     3601 cacatcaggt ctggatccgg tggttcggcg agagcttctt ggggagctgc tcgatttcat
     3661 gggcgacggc gaacggtcgg tcctgttctc tacccatatc acttccgacc ttgaccgggt
     3721 tgcagattac atcactatca ttgtggatgg gcggatacat gacagtgatg agaaggatgc
     3781 cttgcttgag aaggcacgca aggacgagcc gacagcgtcc atagaggata tcgtgatttt
     3841 ggcagcgaga ggggaaaagt gagatgacga atctcatatg gaaagatctc tacgtaaaca
     3901 ggtccatgat attcattgcg atagggcttt tcggggggct cttcgttttt ggagcgctca
     3961 caggctccga agagattgcc gcgtacagct tagggattat gaataccttt tcaccgatga
     4021 tggttccgtt ctgtgctctc aatagcgctg ccaatgagga aaagaaccgg accctcgccc
     4081 tgctccgatc gatgcccatt tccccgcgga cgatagttgc ctccaagttc atctcatctt
     4141 tgcttgcagg cattttgtgg tctgtcatca atatggtcgc ggcctcgtgg ggaaccaggc
     4201 ttttcccagg ggctgggggc gagtttcctc cgagttcatt gacgggccta tacatgatgg
     4261 ccctgacgct tccaatagct gccgtgacat tgatcgcgtt cttcaagtgg aattcgagcg
     4321 cagcgcggac cgctatcatg gcgctatggg tagccctcta tgttatcccg acagcattcc
     4381 gtccggctgg gatgagcatg acggagtttt acctgaagat catgtctccg actccgtcta
     4441 tgatatcagt tgttctcgtt gcttgccttg gagtatacgt tgtgggcatg ctggttgcac
     4501 aagccgtatt tgcccggcgc gaactcgctt gatagggcgc gcagcgagga gccgacagcg
     4561 tccatggagg atatcctcat ttcgaccgcg agaggaggga catgagatga tcagtcttgt
     4621 ctggaaggat ctctacacaa gcagactgat gctgcttctc cccatcgggc ttttcggcgg
     4681 actattagtg tttggagcgc tcacgggtaa tgatgaaatc gcatcattgt acttctcgag
     4741 tttagccaac acctactcca tgctgatggt tccaggctgt gcgtttgcca acgcttcgaa
     4801 cgaggagaaa aaccggactc tctccctctt gagatcgatg cccatatctc cgcaaacgat
     4861 agtcgcatcc aagttcctcg cgccgctgtt tgctggggtg ttgtggtcac tcatcagcgt
     4921 cgctgccctg ttgctcggcg ctaggctttt tcccaaagca gcggggatcg cgcttcgatc
     4981 gactccattc ctgggcccat atttggtggc cttgacgctt ccaacagctg ctatgatatt
     5041 gatcgtatcc ttcgcatgga acgcgaacgt ggcaggcata gctttcgtag tgttatcggc
     5101 agccctctac atgatctcgg caatatttcg cccagctgga atgagcatca tggggttcta
     5161 cctgaagatc atgtctccga ctccgtctat ggtatcaatt gcctttgccg tttcccttgc
     5221 gatatacttt ctggggatgc tggttgcaca ggtaatattc gtccatcgtg aacttggttg
     5281 agtttgcaag aggaagcggg aatggagttg tggctacggt gtatgagcat atcgtcaacc
     5341 ctggcagtat tgtggtagtc ggagcaagcg atgatgtttc aaagcccggc ggcagagtgg
     5401 cgaaaaacat tgttgaccac ggttacgccg gaaatctctg ggcggttaat ccgaaacacg
     5461 atgatgtgct cgggctcgcg accttcaagt ccgtcggtga tcttcctggc actcccgaac
     5521 ttgcgattat cgctatccct gcacgcgggg ttcggggggt tctggaggaa ctggcggcag
     5581 tcgggacgcg agccgtaatc attctgagcg cggggtttgg cgaggtttcg gaagaaggaa
     5641 agcgcgagga gcttgcgctg gtcgagattg cagagaggca cggtatgact ctaataggac
     5701 ctaattgcat gggagtgctg actccgtgct atgcgggagt attcgcaggg cccatcccag
     5761 aacttgagcc ggggtgtgtc gatatcatta gcggatcagg cgctactgca gacttccttg
     5821 cggagctcgc tatagaacgt ggactactgt tgtcttcagt ctttactgtg ggcaacgctg
     5881 cgcaggtggg ggtcgaggac atcctcggca tgatggatga gcactttggg tcgggttcgg
     5941 ctaaggttaa ggccttgtac atggaatccg tgaagaagcc gcaaaagctt ctgcgctatg
     6001 cgcggtcgct gatttctaaa gggtgctcga ttgtgggcat aaaagctggc gcgacaggtg
     6061 ccggaaaacg ggcggccgcc agtcatacag gagcgatggc cagtgatgac acagcagtcc
     6121 aggctctatt cgagaaggct ggtatcataa gggtttccag cagatcagaa ttgctcgacg
     6181 tggcaagcgt tttactcatg gccggcccga tagatggaag aaatgtgtgc gtgcttactg
     6241 atgccggcgg gcctggcgtg cttctcgccg acgagctggc tcgacagggc ctgaaggtcc
     6301 cattgctgcg ggaggcaact caaaaaaggc ttggggaagt gttgtggccg ggaagctcag
     6361 ccctcaaccc cgtagattgc ctatcaacaa gaacccccag ccagatccag caggcggttg
     6421 acgtgatctc ggaagaagag cgcgaccgta tggatgttat gccgctcatc atgggcgatc
     6481 cggggatggg cgggatcgct gagatgtatg acgttattgc ccgtgctatg gacgagtccc
     6541 caattccggt tattccagtc tttccatccc ccataacttc agctctggcg ctggaggact
     6601 tcaaggctcg cgggaagtcc tattttgttg acgaagttca gctcgggcgg agcctggggc
     6661 gagtgataaa ccgtccgcgc ctggatgagc cagtagagac acttcccgga tattgccgtg
     6721 aggcgatctc aagagcgctg caaggggatg aggctcacgg gtctgctcat gcggacaaca
     6781 ccaggacggc tttggcgccc cagacggctg ctgcactgtt gcgtgctgcc ggcataccta
     6841 tccctggcca agcagtggca gttggagtcg aggacttgga cgggatctgc tcggatctgg
     6901 gatttccggt tgtcatgaag gttgtgggcc ccctccacaa gactgatgtg ggcggagttg
     6961 cgctgaacgt tgcgagcatt gaggatgccc gtgatacttg gcggaggttg atgtcaatag
     7021 acggggcaac cggcgttctg gtccaaccca tgatagaggg gacggaactg ataatgggag
     7081 cgacccgcga agagggcttt ggggcactgg tcatgttcgg gcttggcgga gtcttcaccg
     7141 aggttctcaa ggacgtaagg ttcgctctgg cgccgctggg gatggaggaa tgcaagagaa
     7201 tgatcgattc cattcgcggg agacgcctgc tcgacggggc aaggggcgga cgggccgtca
     7261 atgttggcgc agcagctgac atactggcca gggttgggct tttggtctct gattttcctg
     7321 agattgcgga gctggacctg aacccggtta aggggtatgg gtcagagctt tacgcgatag
     7381 atgcccgcat catagtagag agaaggcctg atgaaggcgg ggctttggct cgcttacgca
     7441 gcaggcatca tgctcgctag agtcaggggg cgaggccgcc cggattgcgt gggcgcgaac
     7501 gcgaactttt ccagcgggga agctgtggtg gaacgtacag gtgatgcttg ggcaatggca
     7561 gtcaagctcg acggggtcgt tgtggagtat gagtcgtggg aatatgaagg gcgaaggaga
     7621 aacgaagatg cgcgctggat ggcgtgacca tagggtttgc gggcggcgaa atcgttggga
     7681 tcgaggggcc taacgcgtct gaaaagtcga cgctgcttcg aacgatgtgc ggaatacacc
     7741 ttcccatctc cggcgaggtg ttggtttacg ggaaaaggcc tgacatcttt gactcagact
     7801 tcaagaggcg cgtgggctat gtcagttccc gtcggacatc ttcgtttgcc ctccattgcg
     7861 agctctacgg tatggacaag tacgagtgac aacgggtagc gtcccgcctt gacctggaac
     7921 cattggccgc ccgaccgccg cgcgagtatt cacaggggca gagggccagg gctgagctat
     7981 ggcttgcgct ccttcacaga ttggatctgg tgtttctgga cgaggccaca gaggccctgg
     8041 atctcgagta tctgccgctc ttcttgggaa catgagcggg caaggcggtg gcggcccgaa
     8101 ggcctttttg gcccaaagtt gtgcggatac tggctaagca gaggttcatg ccgctgccgg
     8161 attttctagg ctcagcatta ggagccaccc tcgtattgtt ggcgcagtcc gggatcgata
     8221 gttcagggaa ggcacctcat tgatggatac atgagcgaat ctttgctcac cggtatgctt
     8281 gctccacccc acccgatcgc gctcttggcg gcaaggtcct tttctggagc gcttgtttcg
     8341 cttgcctcca tcgttcctgc gacgcttctg gttgccagtg gttgcagcag gttcttctgt
     8401 ggctgccttt tgtccacatg gccgatacgc cggctgccat tgcgtccggg gtggccatcg
     8461 gaagaatggg aatgcagatt ctgtgggctg tgctgtgtag gaggcgcatg gcgcgctttg
     8521 actgatggac ggatgaatgt gcgtccgcgg gcgctgattc aggcgaggct cagggcttat
     8581 cggatttcgc ccgcggggtt ttgggtgtat gttctctccg tggggctata tcagctcatg
     8641 ggatttgcct tcgtcgttgc caccacatcc aggctgatat cggtgttgta atgccgattg
     8701 cccattttga acccggcgaa atgttcgctt cggttgcggg tttgccatcg ctctattcgg
     8761 cctgtggatc ggaggacgat tcacggcgat caatcttcag tgtacagcct cttcgtgatc
     8821 gcggcctcgc tcgttctgta caacatgggt ctttccgcct gaatcaacct ggcaatggac
     8881 ctcgtgcaat tcgggcagta tccgatgcgc ttctgccccg acgctatgcg catcatcttt
     8941 acctgggtaa tcccccttgc agtggttgcc aatgctccag cctgccttgt agaagcgggg
     9001 ttgacagaga tgccatggca ctttatgcca ggattcatca gatgcggaca tctggccctc
     9061 ctgggttcca atgcaatctt cagataggga ataaggcggt ttttgagata ttggtaaaca
     9121 actgctcccc tgtatcctcc tacggtggca atagagggaa ttgcaaccga acttgcgcag
     9181 ccatataatg ggaggacacg ctgcctgtcc gcggcaagag ggtagagacg aaacccgatg
     9241 ttcgcgggga gccggggcgg agcgggatcg cgagcggcac tttcttacga ggcaggggga
     9301 atggtgtggg cagcccctga actgggtgtg ctgatggtgt cggattcacg tgcgcgtgcc
     9361 tcggactgtg gctgtggtat ttcatctgat ctgccttgct tttctgaagg catgttcttt
     9421 ttgatcatgc tgacagccat caggacaccg ccaacgatta gattgaggta gtaggtgaat
     9481 gcgcgccata ctgtgacgaa gaggcctatg aggccatgcg gaaccacact gctaaatagc
     9541 aggctgaagc cgagctctgc agcgccactc gatccgggtg tgggagcata ggcggctgca
     9601 aggtagaaaa tcagagtgat ccccataatg tgcgtgaact tgggtgttat gcccagaccc
     9661 accaggatta ctgctggaac tatggttatc gtgatccacc acgcgtaact gagccacatc
     9721 actttccaaa gcgctgactt tttgttctcc atgaattctt gcaatgcatt tctgaactcc
     9781 ctgagctcgt gatcgatgcg atcgagcact gcatctaaag tctcgggctt gaagaacttc
     9841 agggtaaact tgttgcgtac aacaggtaca acgataacgt tgatcttttc cgggtgaaag
     9901 atcaggtaga gtgagaacgc aaacaccacg aaatacagga tgatgccgat tgcgaggaga
     9961 acgtacatag ctttgggaat gctccagaag tcagcaaaac caaataacca gatcgatgcg
    10021 gctatgccaa gagtgaagcg ggcaagggcg ttgcatatgg tcttcactgc gataactgcc
    10081 gtagattgcc ctgcattcaa gccttttgca gttagaaggt acgcctgcat aggctcgccg
    10141 ccgctatcga atggtgttac gttagacacg aatgccccca tgatggagat gcgcattccg
    10201 gtcaggatgt ccaagtctcc cccaagcgat cgaatgagag ccctcatccg gagcgaatcg
    10261 aacaaccatg ttactaccac gagtccgcac gcgatagcta gcattgccgg gtctatctgt
    10321 gcaaattcct tccatgacgt tcgtccgccg gtaaactgga gcacaacgta gagccctagc
    10381 acgctgaaga ccacgccaat gagcgcgctc cgtctgagct gagcaggggt tctacggcgg
    10441 gtttgctgtt cctcactcat ggggtttcgc ccccttatag cgggtttttg caaaaacaca
    10501 acatattata tcatagccat ggggcgcaag cacaagagag ttcccgatta tatggaaatt
    10561 aaggcaagct tttgtccctg gctgaagtta gctggaaccg actgcgtact gagcatacaa
    10621 ttgatcaatc ttgactgcca tagatgttta tgcccggcaa gcaacatctc gggcaagtct
    10681 cgacaggttc ctgcgcgatt ccacttaatg attgtctgga tttcaagaac acatatagca
    10741 gcatacgagg tcagggctgt ccacttcttg cgcccctatt gtttcactat gcgcgtgcct
    10801 gcgtttccgt gaatggcctc aaccgccttg tcgagttgcg cgattatcgc cgcctctcct
    10861 ccgcgctcaa cgaaacggcg gcacgcttcg accttgggtc ccatgcttcc tgcattgaag
    10921 tgaccttcct gttggtaacg aatgaggtca tccaattgga ctgtgtgcaa gtcgacctga
    10981 tggggcgttt tgtaatgcag ggctacatgg gccacgtcag ttagtatgag aaggacgtcg
    11041 gcaccaacgt ccctagcaag acactcgccg cctagatcct tgtcgataac ggcctccact
    11101 cccttataga aaccgccctt ttctataacg ggaattccgc ctccgccgtt agcaattacg
    11161 atggcgcccg ttcggaccag tgagaggatg gcgtcttgct ccacgattct gatgggatca
    11221 ggcgatggga ctacacgtct ccagccgcgt ccggcatctt cgatccactt ctctccggtc
    11281 tccttcattc gctgctcggc atagtaagcg ctgtagaatg gccctacagg ctttgtcgga
    11341 tttgcgaaag cgggatcatt cggatccacc accatctgcg tgattacggt cgctacaggc
    11401 ctcgggatcg atcggcgcgc cagcatcttt ccgatggatt gctggagcat gtagccgatt
    11461 tgcccctggg attcggcgcc gcatacatcc aatggcatgg gagggaccct gtcgcggccc
    11521 gcttcttgtt ggatcaggag tgctccgacc tgtggtccgt tgccgtgggt tatcactact
    11581 ctgtggcctt gggctatgag ctcagctact tgttccgcgg tggaatctac attctccatc
    11641 tgctcatcga cagtcccgtt ctgccccggc tgaagtatcg cgttcccgcc tagggcgatc
    11701 acgatggtct tggcattgtc caactcgaac actccagtct tgcagattgt ccgccatgct
    11761 tcggggctct gggcgccttg acgtctgcat catgcattga agatcacctt gcccattgag
    11821 atcgtcgttg ttgccctata agtgttggca tcgaggatag ttatatctgc atccattccc
    11881 cgctggaggc ggccttttcg atcgtccagc cctagctggc gcgctggtgt cgaggaagcc
    11941 atggcgatgg cgtcaggcag cggcacgccg acgagacgaa ccatgttgcc gactgcaaca
    12001 tccatggcta gagtactacc ggcaagttcg cctgattcca ggcgggcctg accctcctta
    12061 acgatcacat gctgggttgc gagggtgtag tctccgtcgg gcagtcctgc tgcgcgcatc
    12121 gagtcggtga ccaaaacaac gtgcgcagcc cctttcatcc tgaccacaat ggaggcggcg
    12181 atcggatgga cgtgcacgcc atcgattatc agttcgcatg tgagatcatc gatcgtcagc
    12241 gctgcgccta caacccctgg ctctctgtgg tggaggccgc gcatgccgtt ataggtgtga
    12301 gtgacatgcc tgattccagc tgccacgccg cgttccatgt cggcataggc tgcatcggaa
    12361 tgtcctgcag aaaccagtat tccctgggac gcgagggcgt ggattatgtc gattgctcct
    12421 ggaatctccg gggctaccgt catggccatg atgtggccgc ctcccgcatc taagagcttc
    12481 tcgaggacag agatgttggg ggccatgatt ccttcttcgg gttgtgcgcc cttacgaatc
    12541 gaattgagaa acggtccttc aacatttatc ccaaggatgc gcgcgccctg agtacagcgt
    12601 gagcctagac ggtcagcatc tatcgcctct gcggcggctt tgcatgcacc tgtcatttgc
    12661 tctagagggg aggacatgat tgtcggcaag aagccaacaa ctccatggga ggcctcaaac
    12721 tttgacatgc agttgagcga atcgaagctc gagtccatgg cgtctgcgcc tccgccgcca
    12781 tggacgtgga gattgatgaa tccgggagct attattccgc ccttagcgtc tatcgttgca
    12841 tcgaccgttt ctttgccgag agaagcggcc gaaccgacat tgagtatcgt gctgtcttct
    12901 atgaggacag ctccgtttgc aatcacggat gtgggtgcta ttacaggtcc gcctgtaatt
    12961 agggttttat gggccattct ttaaacttcc ttccaaaaga gttcgacgca ggcattatct
    13021 tatgccggac gtctaccacc cgaatgttat tctccttagt cgcgctttct tccttcatgg
    13081 ggacggttat cgcatatcat cgagtattgc cgctcagact agtgctaagg gaatggagga
    13141 ctttctgcca tacaactgtg gaagcacggg gcgtaacctc aaaccgaaat cgacaaaagt
    13201 cggctgaggg acgctcacaa gtggccagca ctgtagtggt tgattacagc tgtgagcaag
    13261 gtgcccataa tcagcggctg attaggggcg gatcgacaca attcggggat tggagtgatc
    13321 agggcttgag caagttgcgt gtaggcgtaa taggaacggg catggcattc gaacggctcc
    13381 actatccggc ctacgagagg ctttcagatg tttacgagat cggcgccttg tgcgatcagg
    13441 atgctggcaa attggcggat tggggacagc ggttaaggtt gggcgctgag agcttgtata
    13501 ctgattggcg caagatggtg gccagagatg accttgatgt gatcgacata atggtcccca
    13561 tcgagctcaa ttacgctatc acagaaaggg ttgcgcggga gctggcaggc aagcggaagg
    13621 cgattatctg tgagaaaccc ttggccggca ccttcaagga agcactttca gcaagagagc
    13681 tggcgcagcg atacagtgta ctgatcatga tcgcagaaaa ctacaggtac agtgaggaaa
    13741 tgaacatcat tcgggacctc gttgtgagca ggcgagtggg agatccggtg tatttcatat
    13801 acaaccgaac cgtggatttc ccctcggaca tgtggaaaga tgattttgcc gcaagggagt
    13861 ggcgacagta tcccgagttt cctggcggcg ccattgccga catggcagtg cacgacctgg
    13921 ccgccatgcg ccacattttt ggcgcaatcg atcggctgca ggctttcggc aggccgcaag
    13981 atgcggagtt ctctccatac agtgtaatag tggtaaacat gctctttgca agcggtatga
    14041 tcggccaatt cagcttcttt tgctccggca aggagatgca gcgccctctt gtggggttca
    14101 ggatcttcgg caccgaagga atgatctatc tggaggaccg gaactcagga gtgataaatg
    14161 tcgcttacaa cgacaggcgt tcggaacaga tcccttatga tccccagaaa gcctactaca
    14221 atgagctgct caacctcgcc aacgccctta gaggaaagga gccggtaagt gtaccgccgg
    14281 agatggaata tggagacctg aaaacggtca gagacatact aaagtccatt caggagcaga
    14341 agatcgtcac tgtggacaag actgcggatt atcttcccga ctatggccag ccgggacgcg
    14401 aagggctatg gctacagtag gctagcctca agacgcgtca catggaacgg gcggggctgg
    14461 cggcatagct cggagccaag gagtcaacgt cattttgaca agaaatctgg ctgtatggcc
    14521 tccatatgga ttccgccggc gggccgggct tgtgcccggc ttcttcattt agtgcgccat
    14581 aaggggataa gaagttgtgg cggttgtttc ttcggcctaa cgttcagttt tgttgctagg
    14641 agcggatttg tgcgatccgg catctgcgac aaaaggctct aggtctgaac ggaggattct
    14701 ccaatcccgt cctcgcttga tgccatggag acggccgtct cgcatcattt tctgtatagt
    14761 tgtggtcgaa acggctagta cttccgcggc tttgtctatc gttaccgtcc taggtttggt
    14821 tgaaacggct ttccactttt tctcggtcat gatgacacct ccactactta taataacata
    14881 aagccactcg aaggaggtta tggccaccag aatgggaaca aaatgaatac cgctcgacaa
    14941 ttctattatg gtaggatgtt cttgaagtag ccattcattg gcagttccaa tctggcgcga
    15001 ggaggcacga aataccatgt tcaaggccag gcatctacct ttactgcttt tagcgctcat
    15061 attcataatg tcgatctttg tgtacagggt gctccccgat agagtcccaa tgcacatgaa
    15121 ctggaagggc gagattgacc gttacgggtc gaaggcggaa ggggcctttt tctttcccgt
    15181 cgttatactc attgttcacc tgataatgtc gttggtcttc agggcctaca taaacagtat
    15241 cgatgcggac gcccgtagcg ttgccgcgga tagacttggc atcttcaaaa gccttttgat
    15301 tctgcttttt gcggccatgt atgcagactt cgtaatggtc gccttgggca ggccgtccag
    15361 tatgtacgag tttcttggca tccctgttgc actgcttttc tattacacag gcgttctgat
    15421 gaaggatacg cccagaaatg ggataatggg ggtaaggacg aagtggtcga tggcggatga
    15481 tgaagtatgg gtgaagactc accacatggc gtcgacggtg taccgtgtga gcgctctgtt
    15541 cccactgctc tccctggcat ggccgaacct cttttggttg tggcttgcgg ttccattgat
    15601 cctggctccc accgtgatga tatttgcatc gaggcgttac tatatggagc tggtcggaag
    15661 gaaatgatcg aacagttgaa aaatggcggc ggaatcgcgg gagccttgcg ggctgcagtt
    15721 gactcatgcc ggatttgacc tgaaaaccag gtaattgcga gaatcttcat gagaagagga
    15781 catcgggttg cagggattgc gattgccatg tccgctatat taactatttt ggccgatggc
    15841 gccatacagc tgaccataaa cgggcgggcc ccttgaggcc gataatgtcc ccagagttga
    15901 caatggcaag gtggtggcgc tgttgcagag gcgcttgggg atttgcgggc ccaaaacatc
    15961 atcgcttgag cggcatatca gacttttgga ggaagcgata tcgccatcta ctcccaggga
    16021 ggccgtggag aagtgggccg agacgcgcaa ttgggcgctc cagtatgcgg tgatctatcc
    16081 tgaattgcga gaggtcgcaa gatgtttggt ggtattcgac tcttcaacat ctacaggacc
    16141 taccgtggcc agaatatctg ttgaaagaga caagaatcga gccaggctct cgcggcatat
    16201 gtcggaggag aggtggcatg tggtaaagac ttccgtaaag ccggagtcag actgccagtt
    16261 gaagcgattg gtcaaggact tgattgagga gaagtaagat cttcattttc gacgattagc
    16321 catgaaatca gcaggaagca ggcatcatct cgctgcctgc ttcctgcggg aaatactctt
    16381 gcttgtcatc aatgggaatt accgaccaaa agctcctcgc ccagctgagc tttgaaggcc
    16441 tcaatataga ttccaacagc cactatagac atgccgcctt cactggaagg gtcaggcgcc
    16501 ccgctgattt ctattcatcc acaaccaaag cctcgtagag cacgcgccct gttgcgtttg
    16561 ccggctgtct gagccccatc agcctcgaca tagttggcgc tgcgtcgatg atctgcactg
    16621 gcgaatcgat gacgactccc tgcttcacac cggctccgct tatgagcatg aatacatgcc
    16681 gggaagattc ttcgcccgag tcatgttgcc ccccaaggcc gattagtttt ccggaagtgt
    16741 ggaaatggtt gggcggctca ggccaaatca tgagagatcc gagggacgga tgggctcctt
    16801 ctttcttcag gtcatctgcc gtgaaatagc cgcctacata cggctttgaa gccaatgtat
    16861 ctataagagc cttgtactgg gcgtcagaga tcgcgtttct gaaatagaac tgtaccgaaa
    16921 ggccggcgct taccagcact atgtccgtgt tcttgctggc acgctcgttc ttgcccaaga
    16981 actcgcactt gtagccgaga ctttccacgg tctttatgag atcttggagg ctcgatggtc
    17041 cccagtaggg agtcatgccg tggtctgctg tgaggatgat cgctgtctcc tcgaatattc
    17101 ctagtttctt gagggcgtcg atcaggttcc ccagcccctc atccatgtcg atcatgagtt
    17161 tggtcatctt tgccttccac gaatcataat ctcgcgccac tgaaaacccg tagccaggcg
    17221 aagcgttgtg ccctgcagcg tcgaggtcat cggcgtagat cgccatgaag gtgggccttt
    17281 cggcgggggt tatcatcttt ccattcatcc cgaacacagg tttcaaattg agaatcttct
    17341 ccgcttctgc cacgcgcttc aaccatcccg cgccaggctg gatatacaga tggtagggat
    17401 cgtcagtata ggttccccgc tcctgcaaca tgaattgctg cacagatgcg cacggtaatc
    17461 cgctcttctc cacgacctcg gcaatggtct cagcgtcatt gtgacggccg gtctccttaa
    17521 cgaggttttc cacagggtcg tagtaacggt atgtattccc gtgcacagcg ggccatgtgc
    17581 cagtgactat gctcgtttgc atggggttgg taatgccggg aataccgttt cgggcattgg
    17641 tgaagagtgc tcctcgcgaa gcaagataat tcaggttggg agtgcccggt tggccgggtg
    17701 aattagccca ttcataatac ctgtaagcac aggcatccca gttgatgtag atgacgtact
    17761 tggccagagc gttctgacca agggcatctt gcccggctgc gctgtctgca ccgggctttc
    17821 cgctatttgc ccaggttgtt gagaccagtg tcaatgccaa aacaacaatg ataatgctgc
    17881 ataaaactgt gccgattgtt gccatcccct ttttcatgcc atccggcgct catcgacgta
    17941 tccttgtgga aacgtggggg agagcgcctt ccctccttac tctgctagaa ttatgctctg
    18001 cgcacagagg gtgcgccagg aatgctcact gtgccccgga cggctttggt ccggcggcta
    18061 ctggtcatcc gacatcttcg aatacgcgac tccagtagaa tttgtcgaac acgagcccgg
    18121 cgaggcccag tgcaattccc atatcgccaa gctgtgaagg gactaaccgc acgttcttgc
    18181 gcggggtcaa taccatgcga tctcgaacta ccgtttccac ctgtgataca aactgaggtt
    18241 cttggcctat atctccccca agcaccacca gttccggatc gagcaggttc atcagattca
    18301 caatgccgat tgcggcgcaa tccacgatag aatcgctttc gttgtttcgc ctcacctcgt
    18361 caatggctgg gtatgtaagc gtcaccagac agcctctgtt cccacaagag cattgcttcc
    18421 cgtggatgtc cacggtcatg tgcccaatct cgccagcctg cattcgcgcg ccagcgtaag
    18481 gtttgcctcc caagatcagc cctgccccca gcccgccctc gcaaaccatg acgtatacga
    18541 aatcgtcagc gcttcgggcg aggccatacg agcgttctcc ccacgctgcg gcacgcacat
    18601 ccctttccac ataagaaggc aatgagaact cttgctgcaa gcgggccgct aaaggcagct
    18661 ctttcagacg aaggcgggtc gacttgataa cggtgccggt cgttgtgtca actatgccag
    18721 gaatggacac gcctatccct gccgcctgtc cgccagaccg tggcagcctg gcaagggttt
    18781 tcgctactat ctgaaagatc tggtggtagg tgtcttccgg acttgtcagg tcgagcttgt
    18841 cccttgcagt ccagactggg ttgcccgcta gatctatcac gccgtagagc acgcgggata
    18901 cctgaacatc gattatggcg gcacagcttg ctgaggcgtc gaggtcgagc ataatgggac
    18961 ggcgtccgcc gctgctggca gcagtgccct tttgctctcg cacaatgccg gattccacca
    19021 gttctgctac tatgctagac accgttgatg ggctgagcat ggttagcctg gcaatatcgg
    19081 cacgcgacag agtcccatat ttcctaatgc aatcgatgac tacatctcta ttgagcttct
    19141 tggcgagcgc ggaatcagca gtgcgtttga gtttcatatg tgcttacctc tcagcgtgta
    19201 gtcaggttgc gctatcgaac tggcacaaac ggcgcgaatg acgattgcgc cagtggcgtg
    19261 atttttagag tggcgaataa attcgccatg caactaaacg gcttcgaatc aaatgccttt
    19321 acattatcat atggtacgtt tagtgacaat gcaagattaa gatgacgcca gagacagatc
    19381 tggtctgaac tggccattta acgtgaactt gacaatcgtt gactctgctg aggccaggtg
    19441 gtatattaat agtacattgg catggcagaa gcgccagtac attcgactct cgcagaaact
    19501 atcggattac gctggagacg tacgaccctg cattcccgcg atgggaatcg gccgggaaaa
    19561 ctcccggtga ctctacgaca tccatatggg tgttacaaga tctaaaggag ggtgcagtca
    19621 tgtctaacaa gaagacagga acgattgtcg caaccgccgc tttggccgcg atcatgctgc
    19681 tggcaagcat aggcgtttcg gcgtcagcgg ccgacatctt cgccggatgg agcgagccca
    19741 tgctctggga gcattccggt ggcgacacag agaggatgcc atatttggcg ggcaacgatc
    19801 tttactttgc aaaggattac gacatctact ggtcgacctt tgacgaaacc acaggcaagt
    19861 ggtccgagcc ggagccggtc ccgggaccga tcaacaccgc agccaacgaa gcctgtccct
    19921 gcgtagttgg aaacggaaag gtcctctatt tttcgcgtta tgaccccatc acggattacg
    19981 atttctatcg ttcagaatgg gacgaggaaa aagccgaatg gggcgagccc gagctcatcg
    20041 aagaactgag caccgacgga caggactggg cgatacacgt aaacgaagat gagacgatag
    20101 cgtatgtgat atcgaagata ccttacgacg aggcaaccgt tgtgggcggc cgcgacatct
    20161 ggaaatccgt caaagtcgac ggtgtgtggc agaccccggt caaccttgga gagccgctca
    20221 acagcaacgg tgaggaatgg agcatcttcg tcgattacat gggccgtttt tggatagatg
    20281 gcaaccgcgc ggactccctc ggcaacttcg atatatatgt agccgagaac gagaaagcga
    20341 ctccgatcaa tcttggcgcg ccaatcaaca ctgacgtggc ggagcgcgaa cccacaataa
    20401 acgagaaatt cttcttcttc gctgccaaca agagagaggg aggccatggt ggctgggatc
    20461 tttggtacat ggtgaggctg ccataggctt ttcgtctgct caaattgcag cgatcacaat
    20521 gtcaattggt taggctcgac gtgaaggcag cttgaggttg gcatcgtagc gcgaaggcgg
    20581 cggccttgga aatcgaccta atacagacat ggattgtcaa ggagggctat tgggtgcata
    20641 ccgtggctat ctggggagcc ggcaccatgg gccgggtcca tgcaaagcgt tatgccatga
    20701 ctggcgaagc tcaactgaaa tacataatag acaacgatct tgagaaagcg acccggcttg
    20761 cacaaccttt tgatgccgtt gcaacggtcg atccaggccc cgctgttcgc gatccgcaag
    20821 tggacgtgat cgatgtctgc cttcccactt atcttcacag agaatcagtg gagcttgccg
    20881 caggccatgg caagcacgta ttctgcgaga aacctatagc acttacacta gatgacgcaa
    20941 atgccatgat ttcaacgtgc aagtccaatg gcgtgaaact cgggatagcg catgtagtcc
    21001 gctatttccc ggaatatgaa atggcgcgcg acatgatagc ctctggcagg ctggggcgtg
    21061 tgggcgttgt gcggctttcc cgcacaggtc cgttcccggg tggatggaac gattggtacg
    21121 ccgaccccca gcttagcaag gggctttttg tagatctatc gatacatgat ttcgactttc
    21181 tgaactggtg ttttggccct gtggaacgaa tctgtgcgcg taaggtcgca acgagtaggc
    21241 ccctggagta tggccttgca actctacgga tggaatcggg cgtcatcgct cacgtagagg
    21301 gcagttgggc acatccaagg ccgttttcct acaagttcga gatatctgga agcgaaggca
    21361 tgatagagtg cgacagcgca aagaccaagc ccctaacgtt gctcactctt gcacagcctt
    21421 cctccgattg ctcaaagaat gaaaaggcga gagagggagg ccccgaggcg ggtttgggag
    21481 ttgcaacttc cgagagtccc ctgagggaga gggacccgta tcttcgagaa attcaggatt
    21541 tcctggcgta cgttcgaggc gagcggttgc cccgggttct tcctgaggac gctgtggcgg
    21601 cgttagaagt cagcctggcg gcgacccgct cggcggatct gggagaggcg gtgaaggtaa
    21661 ggtgacggat ggtgcggaga agaagctccg cgttggagta atgagctttg cgcacatgca
    21721 tgcctacagc tatgcccggg ctgtctgctc attgcccgat gcccagctgg ttggggttgc
    21781 ggacgatgac gaggcgagag gcaaagcagc agccgagcaa tacggcattg actatttcaa
    21841 cgacattgag cagctgttgg attcgggggt tgacgctgtc atcgtctgtt ccgaaaatgc
    21901 tcgccacaga gaccacgtgg ttgctgcggc gagagcaggc gcgcatgtac tgtgtgaaaa
    21961 gcctattgct gccgatgtct gcgacggggc cgagatgatt gaggcctgta gagccgcagg
    22021 ggttgtgctg gccactgcct ttcccgtccg tcacgtgcct gcggttgcgc gaacgaaaca
    22081 gcttgtggag gaggggaaga tcggtaaggt gttggccgtc aaggccacaa accacgggcg
    22141 catgccggga gggtggttca tcgagcctga cctttcgggc gggggcgctg tgatcgacca
    22201 tacggttcac gttgcggatc tgcttcggtg gatcctcaat gacgagttca agtcggttta
    22261 tgcggagata gacaccaggt tctacgattt tgacatcgat gattgcggca cactctgtct
    22321 agagatggaa aatggcgtgt ttgctacttt ggacccgagc tggtcgagac cgcgaaccta
    22381 tccaacctgg ggcgatgtta cgatggagat catcgggact gacggggtta tctcgctgaa
    22441 tgcattcgcc caatgcttgg tcgaggccag cgattctcaa ggagccgttg tatacagggg
    22501 gtggggcgac aattccgatc ttgccctcgt tgcggacttc gtctccgcgg cacgcgaggg
    22561 acgtaggccc gctgccgacg gcgtggacgg cctcagggcg ttggaagttg ctctggcagc
    22621 atacgagtct tccaggctcg gggaggttgt cgcactcgat aagttggatt gctagaccgt
    22681 gatttgggta tcagtcaatt gaattatgcg gccgcgtctg aggcgcggtc agcaaatcct
    22741 aggaggcaag aaccatgacc aaaaacatga gcaggtacgg tgcgttgttg ctcgcagtcg
    22801 tgcttctcgt aggactctcc tgcaacgtag cggcgcagaa gaaggtcacg atcgtgcact
    22861 ggcagcatca ctctcctgcg cggctcgaac tggtccagac cttcgcagag gagttcatga
    22921 agcagaaccc taacgtcaag atagagatcg aatcggtgcc tgaaagcgac tacttccaga
    22981 agcttcttcc ggcgcttgcc gcaggatcag gtcccgacac gttccagatt cccacaggcc
    23041 aggtgccagc gtatgccgcc tcaggcgtca ttcagcagat tgacccgaga gtggccaccg
    23101 cccggcagat tgagaatgat tttatcgcgc cggcaatcaa gaggcttaag tatgatggaa
    23161 aatactacgg ttttcccgtt gacacgcaga cgatagtctt gttctacaac ccgaagctct
    23221 tcaaagaagc tgggcttgac cccgcaagac ctccgcagac atgggacgag ttgataaagt
    23281 atgcgcaggc aattcacaag cgggatgcca agggcgttac aacgcaaatg ggcgttgcta
    23341 caggcggata tggccccgtg ttggtctctc tcatgattca aaatggagct ccgctttggg
    23401 ataacaagac gcagctcccc gatttcaact ctccacaagc catagaagcg ctgaagtttg
    23461 cgacggacat ggtgactacg tacaaagttg aggatcccca
//
LOCUS       DAOSAI010000003        22513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_1653_length_22513_cov_4.014249, whole genome shotgun sequence.
ACCESSION   DAOSAI010000003 DAOSAI010000000
VERSION     DAOSAI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 22513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22513
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1653_length_22513_cov_4.014249"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLM83_00235"
     CDS             <1..289
                     /locus_tag="PLM83_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOK69875.1"
                     /translation="CVSSLSPGLLSGFTVGGLSLFGLLDWLAVVVLQPLGGMILAIFV
                     GWVWGKNNVLCEVTNGGEIRFRPGSAWVDVVLKLIAPILIVVIILAGLGLI"
     gene            385..900
                     /locus_tag="PLM83_00240"
     CDS             385..900
                     /locus_tag="PLM83_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69876.1"
                     /translation="MASCLTGESRGGRVVGVALILFMMTAASGCGGNKPSSDPRSTPN
                     GTLSELEKAIRKEDITAILRCYTDPYYVTNDYETTEITHDIARIAYAIIFAAYDYSTW
                     SLADRNISMSGSEAFASCTQKMYFEGEWMSAPAMYRMCIVGSRWYIYEEIVVDSGYDF
                     SPSVRMKDLAK"
     gene            1200..1748
                     /locus_tag="PLM83_00245"
     CDS             1200..1748
                     /locus_tag="PLM83_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69877.1"
                     /translation="MSQHTLVTVQELADMLQLSVATIWRYTRTEQILYVQLLNRQYCY
                     DIEKIMDHLADRVWTYEDYLRLPDEERYYYEVFGGCRFRTTGQSILKTKSCEAHALKD
                     NAYVLRSSGLDTGESTHPGLASDLAALWGRRVFSVSPEESLSDSSACIVRSSDFGRPI
                     RLRYFLRVKRADPVQSLYAKRK"
     gene            complement(1993..2478)
                     /locus_tag="PLM83_00250"
     CDS             complement(1993..2478)
                     /locus_tag="PLM83_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69878.1"
                     /translation="MKKSCVILVALLVILCSYASSSVEASTVIGLKNGAGAGILGLEI
                     ENEFDSGLAVGIVGGLAAVLNPVTLDPIAGVSAAGTVRYYIATSGNVRPFGGGFGGIV
                     AISDGTTNITIPLFGVTGGAELRLGKFRITGELGYGGTVLESEGFIGALASGVSLGYI
                     F"
     gene            complement(2558..3232)
                     /locus_tag="PLM83_00255"
     CDS             complement(2558..3232)
                     /locus_tag="PLM83_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69879.1"
                     /translation="MRCPRSSYTFLILPLALGIMLTTTGCFMGTETIDVEYLPGFQAE
                     RLFYNEQPSIAVNKIWDERPQKDRVGEGYNMYGSKVETWITRQTPTDILEAALIKQLE
                     SMGFVVVMTSGWDLAADSIPEYITSDFVAGGRLKAFWVESRPGLVTVSVNSRVTFDLV
                     IADVETGKVIWAGQFSGSDQPETIIRLDSDMRDSLSRSLTEAVNSAFQDDSVRRAFSN
                     ALQVRF"
     gene            3944..4099
                     /locus_tag="PLM83_00260"
     CDS             3944..4099
                     /locus_tag="PLM83_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69880.1"
                     /translation="MHRLPERSHGSHGRERMRVAFVVVKKMVARERRTGISIYPGFAP
                     RIISRRG"
     gene            complement(4323..4904)
                     /locus_tag="PLM83_00265"
     CDS             complement(4323..4904)
                     /locus_tag="PLM83_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69881.1"
                     /translation="MKRPTRFIIAMVVLLVPLTLTGCGMSPDKTVAKFLDAFKAADSR
                     EMSKYVLGGNTSNLEDFDDQDPEAAEVALSVLSKLTYSIQKPAIDGKTATVPVNITSI
                     DMPRLMANLLPQMFGAALASAFSEDGGESVEELSMRLTLNAINDPNVPMTTTRVHFPL
                     TKERGKWLIMPEDELGVSLSNAVTGNFADLFGD"
     gene            6186..11501
                     /locus_tag="PLM83_00270"
     CDS             6186..11501
                     /locus_tag="PLM83_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOK69882.1"
                     /translation="MGRVFNVFKVRDQLVSDFKDYVTGFIHINDERIRGKVDEEFKHG
                     FLWPEPLIQLNPSYEQTKYVDDLVEEGLLHPECSRIFRRDKTPDSLGKPLRLYKHQEE
                     AIRAASSGGNYVLTTGTGSGKSLAFIIPIVDYVLRNGSGKGIQAIIVYPMNALANSQM
                     EELARYLKHGYHEGREPVTFRRYTGQESMKEREEIQENPPDILLTNYVMLELILTRVH
                     ERGLVWAGQGLRFLVLDELHTYRGRQGADVAMLVRRVREAFEAPNLQCIGTSATMASE
                     GDFRDQQAQVAEVASLLFGSAVVPDHVIGETLCRVTPERDLSDPKFIEELRQCITHPE
                     SFMPKGYEAFRMDPLSIWIESTLGVRKEKTTERLVRQIPMSITGPKGAGRKLADLTGL
                     DERLCTEAIQNQLAASYDPANAHPQTEAAPFAFKLHQMISRGDTAYASIGSPDERYIT
                     LRGQRYVPGEDRSRFLYPLVFCRECGHEYYSVTQVTQTGTGRVCYEPRQFGDVTPDNE
                     RDDRSCRPGYLYIDDDKPWPIDDGEVVARVPDNWVEEMATGRRVKRYYQERVPSLVVL
                     SPDGTVAESTDDERILCAFVPSPFQFCPYCGIAHTSILGGEYGKLSTLATEGRSSATT
                     IISLSAVQSLRKCDELDSSTRKLLSFTDNRQDASLQAGHFNDFVQVGLIRSALYKAMT
                     EVGERGLTHDVLPLRVLDALDLPLESYAYGGEHGLDYQARSRARRSMQSVLEYTLYCD
                     LERGWRVNSPNLEQCDLLRIEYIALDELCADESVWSNKHVSLREASPETRKAACEVLL
                     DYMRHGLAINVECLEPDYGEVVRRQSVQNLIEPWAIDEGEKMKTAAALFPGPRPKTRY
                     GGGDDLYASGRGLYCGYLRRSTTFPDYPEKLSLDEAESVLKCLLDALRYGGLIVEVPE
                     YRRRDKNVPGYQVRASGMMWKAGDGTNPRPDPLRTWPGFENLHKANPFFLEHYTGKAR
                     KLQGIEAREHTAQVSAENRLERERLFREGQLPVLYCSPTMELGVDIRDLAVVNMRNVP
                     PNPANYAQRSGRAGRSGQPALVVTYCSTFSSHDQYFFRRPELMVAGSVLPPRMELANE
                     DLLRAHIHSMLLYEAQISLGSSMVEVLDMSGEKPTLAVNESIASALRDRAILAQTRVP
                     AERVLASLKPQLETVDWYTDRWLEDVLNQAYISFDRACERWRGLFRSAKEQADRNHKI
                     QTDLSCTDNHKKDRAERLHNEAIRQLKLLANEGEHDDSDFYPYRYFAGEGFLPGYNFP
                     RLPLSAYIPGQRGHRGKREFISRARFLAISEFGPRSFVYHEGSRYQIRKVILPPQAVA
                     GDERDLALETIKQCTACGCIHPCNDGDDIVICSNCGQRLGGALSSLFRMTNVSTERAA
                     RINCDEEERQRVGYDIRVGFRFANVGGKPSFRVAQVTCDDEELAAITYAPSATISKIN
                     LGWLKRKEGDVLPGFVLDLETGRWARGSKTLERVDESAADADEMSRNQERVIPYVDDT
                     KNCLIFTPTCMLDESAMATLQAALKSAIQIKYQLEDSELAAEPLPSRSDRRLILFYEA
                     AEGGAGALRNLIDDPQALSEIAREALSICHYDPDTLEDLGAGSDVDEGCEAACYDCLM
                     SYSNQMDHAILDRKHKKVLEFLESMKDSCTEASPVGMPRGEHLETLLAKCDSDLERKW
                     LKFISDLDLRLPTEAQKYLAEANTRVDFYYEIESAPAVAVYIDGPVHEIRDRARVDRD
                     QEAGLAQLGIMCLRFSYDDDWERIIAEHPSIFGKIESGARRGDMQ"
     gene            11507..14350
                     /locus_tag="PLM83_00275"
     CDS             11507..14350
                     /locus_tag="PLM83_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOK69883.1"
                     /translation="MGFGVGTLVRARGREWVVLPGSEEELLLLRPLGGEGEIAGICTS
                     LEPVESAHFDLPDPEHVGESFSCRMLRDAARLTSRAYCGPLRCLGRVAVEPRLYQLVP
                     LLMALKLDPVRVLIADDVGVGKTIEACLIARELLDRGEITRTAVLCPPHLAEQWQVEM
                     AEKFHIEAELVLSSTASRLERNCAMGQSLFEVYPHVIVSLDFIKTDRRREEFLRTAPE
                     FVIIDEAHTCAFGFERRGRHQRYELVSKLAADPDRHMVFVTATPHSGKEDAFRSLISF
                     LDPEFSYYPEDLSGPDNEPRRRRLAQHLVQRRRADIEHYLDAETPFPRRDTQEAHYTL
                     HSEYKQLFDEAIDYARETVADESGGFHRQRVRWWSALALLRALGSSPRAAAATMRTRA
                     GVAEAETVEQADEIGRRTVFDIDADEQLPEGIDIVPGSLAELEGESEEDKESQREKRL
                     LSRMARQAEALEGDKDHKLIKMVGFVEEYIDDGYSPIVFCRFIPTAEYVAEELRKRLP
                     KDVEVACVTGMLPHVLREERVMDLANHKKRVLVATDCLSEGLNLQESFNAVIHYDLSW
                     NPTRHEQREGRVDRFGQKSPSVRVLTYYGLDNQIDGIVLDVLLRKHQTIRSSLGISVP
                     VPLDAKAVEEAILEGLRLRQPEDAVQLSLPGIEEQVYVARSEFEAIWDKSAQREKRSR
                     TVFAQDSLKPEEVLDALEEAQGSMMTAADVERFFVGAVRSSGGAVLCHDGVAVFDLRE
                     MPRALRDALGAPEVFEAAFDLPVPEGVRWLRRTDPFVESMASYVLDTALDAHGETIAR
                     RVGAMMTDAVSTLTTLLLVRYRYDITTYMDIQEASQLAEECIALAYEGLPTQPRWLED
                     HQAQELLDVEPTGNVYREQALYFVRQAVDGYDGLVEHVEEIAQQRAEGLLAAHRRVRS
                     AARMKGVRYRVQPRLPADILGIYVYLPDRRENA"
     gene            14347..18324
                     /locus_tag="PLM83_00280"
     CDS             14347..18324
                     /locus_tag="PLM83_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOK69884.1"
                     /translation="MKGRLNGRFAAVRTEGGLLPPDLIQRIAAGDASLPGLGSGSYHL
                     ARGERLNEAIARSWVRVRGAWGGFCEALEQLPQDDPAVRLTRERWLGVIFQELHYGRL
                     PRASGLEIEGKAYPISHKWGNVPIHLMGYGVDLDKRTPGVAGAASMSPHGLVQEYINR
                     SDGHLWGIVSNGRKLRLLRDNVSLTRQSYIEFDIEAIMRGELYSDFTLFWLLCHQSRL
                     ESEDPSECWLEQWAQIAKEQGARALEELRDAVEDAIERLGSGFLKHPGNQELRRKLRT
                     GELSAEDYKNQLLRLIYRIIFLFVAEDRGLLHPEETDRCVKEVYADYYSVGRLRRLAE
                     RPRGGKHHDLYRSLQVVMEALGSDDGAPEIGLAPMGGGLWAPDFVPDLAHADVSNADF
                     LAAIRKLTFMTRDGVRMAVDYANIGPEEIGGVYESLLELAPDLDADSGHFELVGVSGS
                     ARKTTGSYYTPRELIQSLLDSALDPVLNEAARQPYAEEAILGLKVCDPACGSGAFLIA
                     AAHRMAGKLASVRTGDDEPSPEEVRKALRDVIGRCIYGVDLNPLAVELCKVNLWMETM
                     ESGKPLSFLDHRILVGNSLLGTVPALMEQGIPNDAFRAISGDDKKIASQCRRRNREER
                     DATAMFVADGEGHSVSPRFLQETSGLVEAIAEINVLSNDDVLEIREKDARYKKLLESR
                     DYRGQKFEADAWCAAFVWEKTKNAPEPITTAVFQSIKASPDWLAPEVREEVERITQQY
                     KFFHWHLAFPDVFRLPFAGELPDNPDMGWCGGFDVVLGNPPWERLKIQEKEWFASRRP
                     EITNAPNAAARARMIKELKAAEPWLYKEFMGDLRVADGESHFIRNSGRYPLCGRGDIN
                     TYSIFAETMKNIISLSGRVGAVIPSGLATDYTTRFFFQDIMGAGGLPALVSFYDFENR
                     QGIFPGVHRSYKFSLVTMAGPGQPATEGADFVFFAQRVEDLKDPDRHVRLTMQDIELL
                     NPNTLTVPTFRSKRDAEITKKIYSRVPVLIREAREGKTEENPWGLSFMAMFHMANDSG
                     LFRTWTQLEEEGWRLEGNIFVKDGKKYLPLYEAKMVHHYDHRWATYLRDGDTTRDKTS
                     EEKADPANCVMPRYWVPEEEVEARLAGKWDRKWLMGWRDVCRSTDERTMIAGVVPRVG
                     VGHKFLLMFPSNCSTSTMLCLLANLSSLVLDFAARQKLGGTSMAYFVVKQLAVLPPSR
                     YLSPTPWNSECCLEQWIAKRVATLLMTADEMQESDFDIDGAYEPAMWDDEHRFQARCE
                     LDAAFFHLYGVDRHDVGYILDKFPIVKEHDEKAYGEFRTKHEILRTYDAMSEAIKSGR
                     AYRHP"
     gene            18698..19576
                     /locus_tag="PLM83_00285"
     CDS             18698..19576
                     /locus_tag="PLM83_00285"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOK69885.1"
                     /translation="MKTLADMIQEWQLSIVSILKGLQGYCKVRCWNWSSSWVVSSGVY
                     CFWAELPPEARPIQARVLEEYQRLHDVLQVLLCGQPDDTLRELKQANEVVIGVIKQEG
                     CLHYATADEAFLEATEALKTAVGLISRLYSAEEDTIILVPDTNALLYNPDLDSWDLYE
                     GRQYRLVLLPTVLAELDKHKINHRNESVRKCSERLIRQIKEYRRRGSLVDGVTLRTGK
                     SVIQTVAIEPDASKSLPWLDASNEDDRLLAAMIEVIRRNPRAYCVLVTRDINLQNKAE
                     FARVPYEEPSEPQRAK"
     gene            20036..21076
                     /locus_tag="PLM83_00290"
     CDS             20036..21076
                     /locus_tag="PLM83_00290"
                     /inference="COORDINATES: protein motif:HMM:NF037892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HOK69886.1"
                     /translation="MSINLRGSYRALLGNAIEALSAAVEIYNKPKISYREECSIILLI
                     NAWELLLKALLSKNKQRIYYPKKRNQPYRTYSLKDALTKAEEFFPESVDYHPTRDNIL
                     LLKRYRDTTIHFYNDESLHVPLYALAQTSIVNFRDVVSESFDRDIAEEITLSLLPLSI
                     APPIDSVEFLRACGGNGMQSRRTREFTQSIRNIVIKSEEAGYDTGRILTAFNVQLVSV
                     KKVSSADFVVGVDGSQVGEGRPVLVHKLRDPNITHPYRESDIITSKANPNKAGLELTI
                     GGAKLTQHTFRAITWKYDIRNQPKYCWMAKNRHLVLYSREYVTFLKRLTEDDVKQAIA
                     DYKSWCRSRNHR"
     gene            21487..>22513
                     /locus_tag="PLM83_00295"
     CDS             21487..>22513
                     /locus_tag="PLM83_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69887.1"
                     /translation="MNEVIRSLGTYRSRRNMSRHARTLQKACKSIRNDPFFEEWHFVI
                     MDRDSPYRGLFQECVLGVVACPGWADRSNERLVLMARHVDADELGQSCRPRKIEYAIK
                     TVVLPPPGSLPIALGGSEDTYPPYTESIRLATAFRERACEWEAELAHRMTAPTLDSSF
                     GMAQQGLSSMYADQMPEDYLSEKSRTGEPLMVQNTLEWPQAASQGIPDSAPLRQQVSD
                     MPEYDSAEMQPIRIDVPEATTITQYETRISEVLKGAQFLGRIEMPEEQLEALLGIIAS
                     QLDGVMYPKACARRLAQNYPAAVAYALVQVAAEEYAEGEVWPRIAEHLGGLETAVVNE
                     LGISIERC"
BASE COUNT         5321 a         5374 c         6532 g         5286 t
ORIGIN      
        1 atgcgtctct tcgctttcgc cgggcttgct cagcgggttt accgttggtg ggctcagctt
       61 gttcgggttg cttgactggt tggcagtcgt cgtgcttcag cccttgggcg ggatgatcct
      121 ggcgatcttt gtgggctggg tgtgggggaa gaacaacgtg ctttgcgagg tgacaaacgg
      181 gggagagata aggttcaggc cgggaagtgc ctgggttgac gtagtcctca agttgattgc
      241 tccgattctc atagtcgtga ttatcctggc ggggttgggc cttatctgat attgtcggcc
      301 tttaaagaga aaaagggcaa cggttccatt tcagtgcatg tattagagta ggaggaaatt
      361 cgccaaaaac atggatggag gttaatggct tcatgcttga ctggagaaag tcgggggggc
      421 agggttgttg gagtggcgct gattctgttc atgatgacgg ctgccagcgg ttgcggcgga
      481 aacaagccgt cgtcagatcc aaggagcaca ccaaatggga ccttaagtga actggagaag
      541 gctatccgaa aggaggacat cactgccata cttagatgtt atactgaccc gtattatgtc
      601 accaatgact atgaaacaac ggagataacc catgatattg cgcgcatcgc ctatgcaatc
      661 atatttgcag catacgacta ttcgacatgg tcgttggcgg acaggaatat ctcaatgagc
      721 ggctctgaag cgttcgcctc gtgtacacag aagatgtact ttgaaggtga atggatgtct
      781 gctcctgcga tgtaccgcat gtgcattgtg ggatcaaggt ggtacatcta cgaagaaata
      841 gtggtggatt ccggatatga ctttagtcca agtgtgagga tgaaggactt ggcaaagtag
      901 gaagttgatc ggtacagggc ccgagcgaca ccgacggtac cgtgtccaac ggggtttcct
      961 gcatctcgag atatttgccg agccggagag ataatgcagc gaaaacctct ggcaggtagg
     1021 cgctgtcaca tggcaaaggg ccaagttctg cccggaggcc ggggcttgga tgaatccatc
     1081 atctctgctg cgccgcttgt atctatggcg tggttttcga tagtcgtcaa gcccttggcc
     1141 aactagtcac tggacatcta ttgtcttgtg gctgatgctc agattgagga ggtattgcca
     1201 tgagccaaca tacattggta accgttcagg agctggcaga tatgctgcag ctttcggtgg
     1261 cgacaatatg gcggtacacc cgcaccgagc aaattctgta tgtgcagctg ctcaacaggc
     1321 agtactgtta cgatattgag aagattatgg atcaccttgc agacagagtc tggacttatg
     1381 aagactacct ccggttgcct gatgaagagc gctattacta tgaggtcttc ggaggatgca
     1441 ggttccgtac tactggacag tcgatcctga agacgaaatc atgtgaagca catgcattga
     1501 aagataatgc gtatgtgctg cgcagttcag gtcttgacac gggagagtcc acccaccctg
     1561 ggcttgcatc tgatcttgcg gctctgtggg ggcggagagt gtttagtgta agccccgaag
     1621 agtctctgag cgattccagc gcctgcattg tccggagttc agactttggc cgcccaatca
     1681 ggctaagata cttcttacgg gtcaaacggg cagatccggt ccaatcactc tatgcaaagc
     1741 gaaagtaatg agccaattca aaggagatgg gcgaatacgc gtggagtgcc catgtggatc
     1801 tgatttctcg tggactgcaa acaggttcag tcgtctccgg tagcctcgat acgcggccgg
     1861 atggtgattc cggccgcata cggggttgca agcgaactcc atggttgcct catcagtagt
     1921 gatcaggcac tgactgcatg cagcttttgc acgatggatc tcacgttcca ggaagcattt
     1981 cttaatgcag cactagaaaa tgtagcctag cgatactcca gatgctagag cgccaatgaa
     2041 gccttctgat tccagcaccg taccaccgta gccgagttca cccgtaattc tgaatttgcc
     2101 gagccgcagt tctgctccgc cagtgacacc gaataagggg attgtgatgt ttgttgtgcc
     2161 gtctgaaatt gcgactatgc ctccgaatcc gccgccgaag ggcctaacat tgccagacgt
     2221 tgctatgtag tacctgacgg taccggcggc cgatacacca gctattgggt caagtgttac
     2281 cgggttcaaa actgctgcca ggcctcctac aatcccgacg gccaatccgc tgtcgaactc
     2341 gttctctatt tcgagaccga gtattccggc cccggctccg ttcttgagtc cgattaccgt
     2401 gctcgcttca accgaggagc ttgcataact gcagagaata accaataggg caacgaggat
     2461 cacacacgat tttttcatgc tactttcctc tcttactcaa ttagtcgctc aacctagccc
     2521 taggcggccc gttttctgct aagacttggc tgggccctta gaatctcacc tgtagagcat
     2581 tggagaacgc tctacgaacg ctgtcgtcct gaaatgcact gtttacagcc tcggttagtg
     2641 atcggctcaa tgagtccctc atgtcgctgt ctagcctgat gatggtttct ggctggtccg
     2701 aaccgctaaa ctggcctgcc cagataacct tgcctgtctc gacatcagcg attaccagat
     2761 caaatgtaac ccgcgaattg accgatacag tgacgagccc aggtcttgat tccacccaga
     2821 atgctttcag tctgcctccg gcaacgaagt cagaagtgat atactccggg attgagtcgg
     2881 cggctagatc ccatccggat gtcatgacca ccacaaatcc catcgactcc aactgcttaa
     2941 taagtgctgc ctcgaggata tcagtgggag tttggcgcgt gatccaggtt tccaccttgc
     3001 tgccatacat gttgtagcct tctcccactc gatctttttg agggcgttcg tcccagatct
     3061 tgttcactgc tattgaaggc tgctcattgt agaacagcct ttccgcctgg aagcccggaa
     3121 ggtattcgac atcaattgtt tcagtgccca taaagcatcc ggtggtagtc agcatgatgc
     3181 caagagccag cggaagtatt agaaaagtgt aactgctacg tggacatctc attccgattt
     3241 cctccttgat ttcagtgcta ggcagacaac cacgaagata gcagctatga caattggcca
     3301 ggcgtaacct actataccgg tcaggctgag taggcttagg atgagcgcta cgcctgcgat
     3361 aatccagtac atggcgggca aacaaccacc ccctttttca agtgcaattg tgactacgat
     3421 gcaatgatcg tattttggca tttgagattg gcagatagga tgcaatgtgt ggcgcataat
     3481 acaaactatt atcccaaaac accagtatac cttcctgaga tttcgagaaa gcatgctatt
     3541 gccttgcgta gtcaagttct tgcccttgct tcaagatgac accatcggat acgaacgtta
     3601 tccagcccaa tcttgtctgc atacccggtg aaaggagtgg gatcatcaag gagaaatgca
     3661 tagacggcac gccttatctg gttgtgaagg tcctttgagt ttttgtattg ccgctgtttg
     3721 agaattggcc aggggactgc taaggccttt acttgtaagt ttggccttcg gtagaagcct
     3781 agggaagcgc cgtgcggcgt ggagccggcc aaggttcgcc gcgggggcga gcaggttttg
     3841 cagaccagtc taaaacttag acacccgcgc ccgaaaagcg actaaataat agacacattc
     3901 cttgaaggca ggcccaagtt gccctttcat atgagtgcgt tgaatgcata gactgccgga
     3961 gcgctcacac ggatctcacg gtcgggagcg tatgcgcgtc gctttcgtgg ttgtaaagaa
     4021 gatggttgcc cgcgagcgtc gaaccgggat ttcgatctac cctggctttg ccccgaggat
     4081 catatcccgg cgcggctgag tgctacgggc gtttatggtt aaggccaata tgtgtacgtt
     4141 gtcgatgcac aaatgcgaac caattaccgc actgcgacat aggtgcaaag acgtgtctac
     4201 gcctagcaga catcatatgg caggctaacc gatagcgcaa aaacttattg ggagttactt
     4261 agaggatgca ggttccgcac tacttgacag tcgatcctga agatgacatc attcggggcc
     4321 ttctagtccc cgaacaagtc tgcgaagttc cctgtaactg cgtttgagag ggaaacgcct
     4381 agttcgtcct cgggcataat cagccatttg ccgcgttctt tggtaagtgg aaaatgcaca
     4441 cgtgtggtgg tcatcggaac attagggtca ttgatggcgt tcaaggtgag ccgcatgctc
     4501 aattcctcca ctgattcacc gccgtcttcg ctaaaggcgg atgctagtgc tgcaccaaac
     4561 atctgtggca agaggttggc catgagcctg ggcatgtcga ttgaggttat gttcacaggc
     4621 acggtagcag tctttccgtc tatcgcgggc ttttggatcg aataagtaag cttgctcaag
     4681 acggataagg ccacttcagc tgcttcgggg tcttggtcat cgaagtcttc taggtttgag
     4741 gtgtttcctc ccaacacgta tttgctcatt tcccttgagt ctgccgcttt gaatgcatcg
     4801 aggaacttgg ccactgtctt atcaggagac atgccgcatc cagtgagggt caggggtacc
     4861 agcaagacaa ccatggcgat aatgaacctg gttggtcttt tcatcagtca cactccttgc
     4921 atgttttgta taggtactga tcgtctcatg gtcagaagct gctgacagct tcaattatgt
     4981 tctgaattcc cagccattgg gttgcagggt gtttgagcta gtagcgaatt agcgtttagc
     5041 ctcgattgtt atggtccacc catacttgga gcggttggcg ccttcaagga tatgggcgat
     5101 gctgcggtcg agtgtttcga ggccgctgcc aggctgatgg ctcaggtgtt tgcgtataga
     5161 aggatccgtt gtctcgaccg gatcatgtat ttcgctccct tcgtttcgaa cttcggccgt
     5221 ctgcagccat ggggtatctt ctgttgaggc cgcttaggtg cagaccagaa caagcaggcc
     5281 tgacaccatg gctttgcggg agaacatggt ttattactcc ttttttggtc tcacatatct
     5341 atctacgcta atcattcttt ttggccttcg ttatcatctg aaacccgcga tgaagcaaga
     5401 aactggcggc ccttgtccgc tgtggcagaa ccttgcgcag cgttggctgt gctgacgggg
     5461 aaatcgaaca catcaggctt atgtgtgcgc gtgtcagaga agaagggatt ttgtaagctg
     5521 ggcaagcccg tcttcgggac ggcagttgtt gatgcgagca tctcttgcgc atgcgtgtaa
     5581 tctagtggtg gccgcttcat atggtttgta agttggaggt tggtgtacaa ggaagcttgt
     5641 agacatttcg cgacagaagt cctctgtgca gaaccagaac ctgatgccgt cgagtatctt
     5701 gatatggcat ttcggttgaa cgtgctaacg gctgccgccg gcaaggagtc ccctaatgtg
     5761 gctactaggc ggcgcccacc ggacgaaact gtggggagat ggcactcaat aatctcatat
     5821 gagtcgtgtg cagcaccgaa aacgtactca caagcttacc caagaatact aaggaacctt
     5881 aattgcgagg ggaatcacaa cggcaattta caccactcga caagatgtga tcgctgagtg
     5941 cgtacaggga agccttttct tgcttgcctt tacccttggc tattcagccg gttacaggct
     6001 gtccgctgtt actacattgc tttgacattc atatccagtc actacctggc taacgtcact
     6061 aaaacggcag ccaaaataga ggacaaacaa ggaataagtg agaatatcat atttaattac
     6121 aactgttgca ctacgatgcc agatattcca gccaaagaat atgagatgcg ggtaggtggg
     6181 cacgtgtggg cagagtgttc aatgtcttta aggttcgaga tcagctcgtc agtgatttca
     6241 aggattacgt tactggattc atacatatca acgatgaacg cataagagga aaggtcgatg
     6301 aggagttcaa gcatggcttc ctatggccgg agcctctgat acaactgaac ccctcctatg
     6361 aacagacgaa gtatgttgat gatcttgtgg aggagggact gcttcatccc gaatgcagca
     6421 ggatcttccg gcgtgataag acccctgaca gcctcggcaa accgcttcgc ctttacaaac
     6481 accaagagga agcgattcgt gcggcaagca gcggaggcaa ttacgttttg actaccggga
     6541 caggttccgg caagagcctt gctttcataa tccccatcgt ggattacgtc ctgaggaacg
     6601 gttcaggcaa gggtatccag gcaattatcg tctatcccat gaacgccttg gcgaacagcc
     6661 agatggagga gctcgccaga tatctaaagc atggttacca tgagggccgc gaaccagtta
     6721 ctttccgacg ctatacaggg caagaaagca tgaaggaacg cgaggaaatc caggagaatc
     6781 ctcccgacat tctccttaca aactatgtca tgctagaatt gatactcacg cgcgtacatg
     6841 agagagggct ggtttgggcg ggacaaggcc ttcgttttct tgttctcgat gaattgcata
     6901 cctatcgggg ccgtcagggt gccgatgttg cgatgttggt gcgtcgggtc cgggaggcat
     6961 tcgaagcccc gaacctacaa tgtattggca catctgcgac tatggccagt gaaggcgatt
     7021 tcagagatca gcaagcccag gtggcagagg tggcgagctt gctctttggg agcgccgttg
     7081 ttccggatca tgttatcggc gaaacgctat gccgtgtcac ccctgaacga gatttgagtg
     7141 atcccaagtt catagaagag ctcaggcaat gcatcaccca tccagagtct ttcatgccta
     7201 aaggctacga ggcattcaga atggatcctt tgtccatatg gatagagagc acgcttgggg
     7261 tgcgcaagga aaagaccact gaacggctcg ttcggcaaat accgatgagc ataacaggac
     7321 cgaaaggcgc ggggcggaag cttgcagatc tcaccgggct cgacgaacgg ctttgcacgg
     7381 aggcgatcca aaaccagcta gcggccagtt acgatcctgc aaatgctcat ccacagactg
     7441 aggcagctcc attcgctttc aaacttcacc agatgataag cagaggcgat acagcctacg
     7501 cgtcgatagg atctcctgac gagcgataca taactcttcg agggcagcgt tacgttccag
     7561 gagaggacag gtcgcgattt ctgtaccccc ttgtcttctg ccgcgaatgc ggccacgagt
     7621 actacagtgt aacgcaggta acccagactg gaactgggcg ggtgtgctat gaacctcgtc
     7681 agtttggtga cgtgactccc gataacgaac gggatgacag atcgtgccgc cctggctatc
     7741 tctacattga cgatgacaag ccgtggccga tcgatgacgg tgaagttgtg gcgagggttc
     7801 ccgataactg ggtggaagaa atggcaaccg ggcggagggt gaaaagatac tatcaagaga
     7861 gagtgccaag ccttgttgtt cttagtccag atgggacagt ggccgaatcc acagatgatg
     7921 agcgcatcct gtgtgcattc gtgccgtctc cattccaatt ttgcccgtac tgcggaattg
     7981 cgcacacgtc aatcttgggg ggtgaatacg gcaagctgtc taccctcgct acagaaggac
     8041 ggagcagcgc cacaacgatc atcagcctgt cagcagtcca atcgctgcga aagtgtgatg
     8101 agctcgacag cagcacccgc aaactattga gtttcactga caacaggcag gatgcctcgc
     8161 ttcaggcagg ccatttcaat gactttgtac aggtggggct gatccgctcc gccctataca
     8221 aggccatgac ggaggtggga gagagagggc ttactcacga tgtgcttcct cttagagtcc
     8281 ttgatgcctt agatcttcct ctagagtcgt atgcttacgg gggtgaacat ggtcttgact
     8341 atcaggctcg atcccgggct cgaagatcca tgcaaagcgt tttggaatac accctctatt
     8401 gcgacttgga acggggttgg cgagtcaact cccccaatct ggaacaatgc gatctgctca
     8461 ggatcgagta tatagccttg gatgaactgt gcgcggatga aagtgtctgg agcaataaac
     8521 atgtgtctct gagagaggca tcccctgaaa ctaggaaagc cgcatgcgag gtcttgcttg
     8581 attacatgag gcatggtctt gcgatcaatg tggaatgctt ggagcctgat tacggggaag
     8641 ttgtccgacg ccaaagcgtg cagaatctca tagagccttg ggctatcgac gaaggggaga
     8701 aaatgaagac cgccgcagcc ctgtttcccg gccctcgtcc gaaaacacgg tatggtggag
     8761 gcgacgatct ctacgcttcc gggaggggct tgtactgcgg ttacctccgg agaagcacaa
     8821 ctttcccgga ttatcccgag aaactcagtt tagatgaggc cgaaagcgtg ttgaagtgtc
     8881 tcctggacgc tcttcgctac ggagggctta ttgttgaggt tcccgaatac cgccggcggg
     8941 acaaaaacgt tccaggctat caagtgaggg ccagcgggat gatgtggaag gctggagatg
     9001 gaaccaatcc caggcctgat cccttgcgca cgtggcctgg ctttgagaat cttcataaag
     9061 ccaatccgtt cttcctggaa cactacactg ggaaggccag aaagcttcag gggatagaag
     9121 cgcgtgagca taccgctcag gtatctgccg aaaacaggtt ggaaagggag agactcttcc
     9181 gcgaagggca gcttccggtg ctttattgct cgcccaccat ggaattgggt gtcgatatca
     9241 gagacttggc cgtggtgaac atgcgcaacg ttcctccgaa ccctgcaaac tacgcgcagc
     9301 ggagcggacg cgccgggaga agcggacagc ctgcccttgt ggtcacgtat tgctctacct
     9361 tcagctctca cgaccaatat ttcttccgcc ggccggagct gatggttgca ggatctgttc
     9421 tgccgccacg catggaactt gcaaatgagg acctacttag ggcgcacatc cactcaatgc
     9481 tcctatatga ggcccagatc tccctagggt catcgatggt cgaggtattg gacatgtcgg
     9541 gcgagaagcc gactcttgct gtaaatgaaa gcattgcgtc tgccctgcgg gatagagcta
     9601 tactggctca gactcgtgtt cccgcggaac gggttcttgc ttccttgaag ccacagcttg
     9661 agacggttga ttggtataca gatcgttggc ttgaggatgt gctcaaccaa gcctatatca
     9721 gcttcgaccg cgcatgtgag cggtggaggg gccttttcag atccgccaag gaacaggcgg
     9781 ataggaatca caagattcag acggacctct cgtgcaccga caatcacaag aaagatcggg
     9841 cggagaggtt gcataacgaa gcgattcgcc agctcaagtt gttggccaac gaaggagagc
     9901 atgacgactc tgatttctat ccgtaccgct acttcgccgg agaaggcttc ttgcctgggt
     9961 acaattttcc tcggctgcct ttgtcggcat acatccctgg acagcgaggg cacagaggga
    10021 agcgggagtt catctcgcgc gcgcgtttct tggccatatc cgaattcggc ccgagatcct
    10081 ttgtttacca cgaaggctcc cgataccaga ttcgcaaggt gattctgccc cctcaggcgg
    10141 ttgcagggga tgagagggat ctcgcgctcg agaccatcaa gcagtgcact gcttgcggct
    10201 gtatccatcc gtgcaacgac ggggatgaca tcgttatttg ctccaactgc gggcagcgac
    10261 tgggtggcgc actttcgagt ctattcagga tgaccaatgt ttccactgaa cgagctgcca
    10321 gaatcaactg cgacgaggaa gagaggcaga gagtgggtta cgacatccgg gtaggtttca
    10381 ggttcgccaa cgttggaggc aaacccagct ttcgggtagc tcaggtgaca tgcgacgacg
    10441 aggagcttgc cgctattacg tatgcgcctt cggcgaccat ttccaagatc aacttgggct
    10501 ggctcaagcg caaggagggc gatgtgcttc cgggatttgt cttggatctg gaaacaggcc
    10561 gttgggccag gggcagcaag acgttggagc gagtcgacga gtctgctgcc gatgcagacg
    10621 agatgtcgag gaaccaggaa cgcgtcattc cctatgttga tgacacaaag aattgcctta
    10681 tattcactcc cacctgcatg ctagacgaat ccgccatggc aacccttcag gctgcgctga
    10741 agagcgcgat tcagatcaaa tatcagctgg aggattcgga acttgctgct gaacctcttc
    10801 cctcgaggag tgatcgacgc ctgattctat tctatgaggc tgctgagggc ggcgctgggg
    10861 ctctcagaaa tctcatcgat gatccgcagg ctttgtctga gattgccaga gaagccttga
    10921 gcatatgcca ttacgacccc gataccttgg aggatctggg cgccggttcg gatgttgatg
    10981 aagggtgcga ggctgcgtgc tatgactgcc tgatgagtta ttcaaaccag atggaccatg
    11041 cgattctcga cagaaagcac aagaaggtat tggagtttct ggagagcatg aaggactcgt
    11101 gtactgaggc ctctccggtg ggtatgcctc gtggggagca ccttgagact ttattggcca
    11161 agtgcgactc cgatcttgaa cgcaaatggc tgaagttcat atccgatctc gacttgaggc
    11221 ttcctacaga ggcacagaaa tatctggccg aggccaatac gagggttgac ttctactatg
    11281 agatagagtc agctcctgct gtggccgtat acatagacgg tcctgttcac gagattcgag
    11341 atcgtgcgcg ggtcgacaga gaccaggagg cgggccttgc acaacttgga atcatgtgcc
    11401 tgaggttcag ctatgacgac gactgggaac ggatcatagc agagcacccg agcattttcg
    11461 gcaagatcga aagtggtgca cggagaggtg atatgcagtg aatagcatgg gtttcggagt
    11521 aggaactttg gttcgagcaa ggggccgtga gtgggtggta ctgccaggct ctgaagagga
    11581 actcctcctt ctcaggcctt tgggaggcga gggggagatt gctggtatat gcacatccct
    11641 tgagcctgta gaatctgctc actttgatct gcctgatccg gagcatgttg gggagtcttt
    11701 ttcctgccgg atgttgcggg atgcggcaag gctcacatcc cgcgcctact gcggtcctct
    11761 gcgctgtttg ggcagggttg ccgtggagcc ccgcctatac cagcttgtac ccttgttgat
    11821 ggcgctcaaa ctcgatccgg tcagggtgct cattgcagac gatgtagggg tgggcaagac
    11881 gattgaggct tgcctcatag cccgcgaact cctcgataga ggagagatta ctcgaaccgc
    11941 tgtgctctgt ccaccacact tggcagagca gtggcaggtg gaaatggccg aaaagttcca
    12001 cattgaagct gagcttgttc tttcgtccac cgcatctcgc ctcgaacgca actgcgctat
    12061 ggggcagtct ctcttcgagg tgtatccaca cgtgatcgtc tctcttgatt ttattaagac
    12121 agaccgcaga cgcgaggaat tcctcaggac ggcgccggag ttcgtgatca tagatgaagc
    12181 ccatacatgc gctttcggct ttgaaagacg tggaaggcat caaaggtatg agctggtgtc
    12241 gaagcttgca gccgatcctg accgtcatat ggtgtttgtt acagccactc cgcacagcgg
    12301 caaggaagac gcgttccgtt cgctcataag ctttctcgat cctgagtttt cctattatcc
    12361 ggaggatctt tcaggacccg acaacgagcc ccgtcgccgc cggttggctc agcacttagt
    12421 ccagcgtcgg cgggctgaca tagaacacta ccttgacgct gagactccct ttcccaggcg
    12481 ggatacgcag gaagctcact acactttgca ctccgagtac aagcagctgt tcgatgaggc
    12541 cattgattac gcaagggaga ctgtggcgga tgaatcggga ggttttcaca ggcagagggt
    12601 gagatggtgg tcggcgctcg ccctccttcg cgccttggga tcgagcccta gggcagccgc
    12661 ggctacgatg aggacgagag cgggcgtcgc tgaggccgag acggtggagc aggcagacga
    12721 gatcgggagg cggaccgtct ttgatattga tgcagatgag cagcttcctg aagggatcga
    12781 catagtgccg gggagcctag cggaattgga aggcgaatcc gaggaggata aggagagcca
    12841 acgcgaaaag cggctgctat cacgcatggc gcggcaggca gaagccttgg aaggcgataa
    12901 ggatcacaag ttgatcaaga tggttggttt tgtggaggag tatatcgatg atggatatag
    12961 ccccatagtc ttttgccgtt tcattcccac cgctgaatac gtggcggagg agttgaggaa
    13021 gcgtcttccc aaggacgtgg aagtggcttg tgtaacaggg atgcttcccc acgttctcag
    13081 agaagagcgg gtgatggatc ttgctaacca caagaagcgg gttctggtgg ctactgattg
    13141 cttgtcggaa gggctcaacc tgcaggagag cttcaacgct gtcatccact atgacctatc
    13201 ctggaatcct acgaggcatg agcaaagaga gggccgggtc gacaggttcg gtcagaagag
    13261 cccaagtgtt cgcgtcctta catactatgg cctcgacaac cagatagacg gaatcgtcct
    13321 cgatgtcctc cttcgaaagc accagacgat cagaagcagc ctgggcattt cggtgccagt
    13381 gccgcttgac gcgaaggctg tggaggaagc tatcttggaa ggacttcgcc tgcgacagcc
    13441 ggaggacgct gttcagcttt cgttgccagg cattgaggaa caggtctatg tggccaggtc
    13501 cgagtttgaa gcgatttggg acaagtccgc ccaaagggag aagcgatcga gaacggtgtt
    13561 tgcccaggat tcgttgaaac ctgaagaggt tctggatgcc ctcgaggagg cgcaaggctc
    13621 gatgatgacg gctgccgatg tggagcggtt cttcgtaggg gcggtgagaa gctcgggagg
    13681 cgcggtgctc tgccatgatg gtgtggcggt tttcgacctg cgtgagatgc ctcgcgcctt
    13741 gagagatgct ttgggtgcgc cagaagtctt tgaggcagcc ttcgatttgc cggtccctga
    13801 aggggttcgc tggcttagac ggactgatcc gtttgtggaa tcgatggcct cctatgtctt
    13861 ggataccgcc ttggatgcgc acggagagac gattgcgcgc cgggttggcg cgatgatgac
    13921 cgacgcggtg tccacgctca cgacgctgct tctggtgagg tacagatatg acataacaac
    13981 ctacatggac atacaggagg cttcgcagct ggctgaggag tgcattgccc tagcctatga
    14041 ggggcttcct actcagcctc gttggcttga agatcaccag gcccaggagc tcctcgatgt
    14101 ggagcctacg ggcaatgtct accgtgagca ggctttgtat ttcgtgaggc aagcagtaga
    14161 tggctacgat gggcttgtag agcatgtgga ggagattgcg cagcagaggg ctgagggcct
    14221 ccttgcggct cacaggcgtg tgcgttcggc tgcgcggatg aagggtgttc gctacagggt
    14281 tcagccccgt ctccccgcag acatactggg catctatgtt tatctgcccg acaggaggga
    14341 gaacgcatga agggacgtct taatggacgc tttgccgcag tgcgaactga gggcggtctg
    14401 cttcctcctg acctcattca gcggatagct gcaggagacg cctctctgcc cggccttggc
    14461 tcagggtctt atcatcttgc ccggggggag aggttgaatg aagcgattgc ccggtcgtgg
    14521 gtgagggtgc gtggcgcgtg gggaggattc tgcgaagctt tggaacagct tcctcaggat
    14581 gatcctgcgg taaggctgac gcgagaaaga tggcttggcg tcatattcca ggaacttcac
    14641 tatggaaggc tccccagggc cagtgggctg gagatagagg gaaaagccta cccaatatcc
    14701 cacaagtggg gcaatgtacc catacatttg atgggttatg gagtggacct ggataagcgc
    14761 actccggggg tggcgggggc ggcttctatg agcccacatg ggcttgttca ggagtacatt
    14821 aaccgcagcg atgggcatct ttggggcata gtgtcgaatg ggcggaagct caggctcttg
    14881 cgtgacaacg tgagcttgac caggcaatca tatatcgagt tcgacattga ggcgataatg
    14941 agaggggaac tgtactcgga cttcacgctg ttttggttgt tatgccatca atcgaggctg
    15001 gagagcgagg atccgtctga atgctggctg gaacagtggg ctcagattgc aaaggagcag
    15061 ggagctaggg ccctcgagga gctgcgcgat gccgtggagg acgctattga gcggctcggg
    15121 tccggatttt tgaagcatcc tggcaaccag gagctgcgca gaaaactgag aacaggagag
    15181 ctttcggcgg aggattacaa gaaccagctt ctacggctca tctatcgcat catattcctc
    15241 ttcgtagcgg aagatcgggg tttgctccat cctgaggaga ctgacaggtg cgttaaggag
    15301 gtctatgcgg attactactc tgtgggaaga cttagaaggc ttgcggagcg cccccgcggg
    15361 ggcaagcatc atgatctata tcgttcgctt caggtggtga tggaggcgct cggatctgac
    15421 gacggcgctc ccgagatcgg ccttgcgcca atgggaggcg gactttgggc gccggatttt
    15481 gtgcccgact tggctcacgc ggatgtctcc aatgctgatt ttttggccgc catcaggaag
    15541 ctgacgttca tgactagaga tggcgtgagg atggctgtgg attacgccaa catagggcct
    15601 gaggagatag ggggagtata cgaatctctc ctcgagctcg cgcctgactt ggacgcagat
    15661 tcagggcact tcgagttggt tggggtctcc ggcagtgccc ggaagacgac gggttcgtat
    15721 tacactcctc gagagctgat ccagtctttg ctcgactcgg ctttagatcc tgtgttgaat
    15781 gaggcggctc ggcagcccta tgctgaagag gctattttgg ggctcaaagt gtgtgaccca
    15841 gcatgtggca gcggggcttt tctaatcgct gcggcccacc gaatggcggg gaagcttgct
    15901 tctgtaagga ctggggatga tgagccttca cctgaagagg tgagaaaggc tctgcgggat
    15961 gtgatcggcc gctgcatcta cggagtggat ctcaatcctt tggctgtgga gctttgcaag
    16021 gtgaacctgt ggatggagac gatggaaagc ggcaagcctc tctcattcct tgaccacagg
    16081 atattggttg gaaacagctt gctcggaaca gtgcccgccc tcatggagca ggggattccc
    16141 aatgacgcgt tcagggccat ttcaggcgat gataagaaaa ttgcttcgca gtgccgcagg
    16201 aggaaccgcg aggaaaggga tgccaccgcc atgtttgttg cagatggcga ggggcacagc
    16261 gtgtcgccgc gttttctcca agaaacatcg ggtttggtgg aggctattgc cgagatcaat
    16321 gtactatcga atgatgacgt tttagaaata agggagaagg acgcgcgtta caagaagctg
    16381 ctcgaatcgc gcgattacag gggacagaag ttcgaggctg atgcctggtg tgccgccttc
    16441 gtgtgggaga agacaaagaa tgcgcctgaa cccataacga ctgcagtgtt tcagtcgatc
    16501 aaagcaagcc ccgattggtt ggcgcccgag gttcgcgagg aagttgagag gataacccag
    16561 cagtataaat tctttcactg gcacttggca ttccccgacg ttttccgcct tcctttcgcg
    16621 ggagaattgc ccgacaatcc tgacatgggg tggtgtggcg ggttcgatgt tgtgctaggg
    16681 aatccgccgt gggagagact gaagattcag gagaaagaat ggtttgccag caggcgcccg
    16741 gaaatcacta atgctcccaa tgcggcggct cgagctcgga tgataaagga gttgaaagcg
    16801 gccgaaccgt ggttatacaa ggagttcatg ggtgatctgc gcgtggctga cggggagagt
    16861 catttcatca ggaatagcgg tcggtacccc ctttgcggac gcggggacat caatacatac
    16921 tccatatttg cggagacgat gaaaaacatc atctcgcttt caggaagggt aggggccgtg
    16981 ataccttcgg gccttgccac ggactacaca acgaggttct ttttccagga cattatgggg
    17041 gcaggtggcc tcccagctct tgttagcttc tatgactttg aaaaccggca ggggatattt
    17101 ccgggagttc atcgcagcta caagttcagc ctggtgacga tggctggccc ggggcagcca
    17161 gctacggaag gcgcggattt cgtgttcttt gctcaacggg ttgaggacct gaaggaccca
    17221 gaccgccacg tgcgtctgac catgcaagac atcgaattgc tcaaccccaa caccctcacg
    17281 gttccgacat tcaggtcgaa gcgggatgcg gagatcacca agaagatcta cagccgggtg
    17341 cctgtgctta ttagagaggc tagagaagga aagaccgagg agaatccgtg gggcttgagc
    17401 ttcatggcga tgtttcacat ggccaatgat tccggcctct tccgcacttg gacccagctc
    17461 gaggaagaag gttggcggct agagggcaac atctttgtca aggacgggaa gaagtatctg
    17521 ccgttatatg aggccaagat ggtgcatcat tatgaccata ggtgggcgac gtatctgcgg
    17581 gatggggata ccacacgaga caagacgtct gaggagaagg cagatcctgc aaactgtgtg
    17641 atgccgcgat actgggtgcc tgaagaggaa gttgaagcgc ggcttgcggg gaagtgggat
    17701 aggaagtggt tgatggggtg gagggatgtc tgccgaagca cggatgagag gacgatgatt
    17761 gctggagtag tgccgagggt gggggtgggg cacaagtttc ttctgatgtt cccatcgaac
    17821 tgcagcactt ccacgatgct gtgtcttctg gccaatcttt cgtctctagt gctagacttt
    17881 gctgcaagac agaaacttgg aggtacatcc atggcatact tcgtggtcaa gcaactagct
    17941 gtgcttccac cttcaaggta tctgtcgccc accccatgga attctgaatg ttgtctggag
    18001 caatggatag cgaaacgagt agcaactctc ctcatgaccg cagatgaaat gcaggagtcg
    18061 gattttgaca ttgatggagc gtatgaacct gccatgtggg atgatgaaca ccgattccag
    18121 gctaggtgcg agctggatgc ggcgtttttc cacttgtatg gcgtggacag gcatgatgtt
    18181 ggatacatcc tggataaatt ccccatcgtc aaggagcatg atgagaaagc atatggagag
    18241 ttccgaacaa agcatgagat tctgagaaca tatgatgcta tgtcggaagc aatcaagagt
    18301 gggcgagcgt acaggcatcc atgacaaaga gtgattggca aggaagcgct ctcgccttgt
    18361 ttcattaggt tcgagttttc atcgttgata catgtatgat tgaatcgaga tgtcggaatg
    18421 tttttgccag ctgcagtgga gaggcttcca gaggaagcaa tgctatctgt aatgtgtggt
    18481 agtggacgtg gcaacagtgg tacgtgcatt gctgcgacag tacgcttgag tctcagggag
    18541 tggatggctt gccaaccttg ggaattgcag tgagggagca tgggtcatat gcagactctg
    18601 ggaatgaaac gggtgcccga tttggttgaa aacgcaaatt gggcaagagg ccaccacatt
    18661 gaacggcaga caccggaatc atggagtggt gtgatagatg aagacgcttg cagatatgat
    18721 tcaagagtgg cagttgagta tcgtctcgat tctcaagggg ctacagggtt actgtaaggt
    18781 cagatgttgg aattggtcca gcagctgggt tgtatcaagc ggtgtttatt gcttctgggc
    18841 agaacttcct cccgaagcac gccctattca ggctagggtt cttgaggaat atcaacgatt
    18901 gcacgatgtt cttcaggttc tgttgtgcgg ccagccagat gacactctac gagaattgaa
    18961 acaggcgaat gaagtggtaa ttggtgtgat caagcaggaa ggctgtcttc attatgccac
    19021 tgctgatgaa gcttttttgg aagccacaga ggccctaaaa accgcggttg gccttatttc
    19081 acgtctttac tctgctgaag aagacacgat aattctagtg cccgacacga atgcgcttct
    19141 ctataaccct gatcttgata gctgggattt gtacgaaggg cggcagtaca ggcttgttct
    19201 tctgccaaca gtactggccg agctagataa gcacaagatc aaccacagga acgagagtgt
    19261 gcgcaaatgc tcagagcggt tgatcaggca gattaaggag tatcgcagac gcggaagtct
    19321 agtagatggg gttacacttc ggacaggaaa aagcgtgatt caaacggttg ctatcgaacc
    19381 tgacgcaagt aaatcgcttc catggctcga cgcatcaaac gaggatgacc gactactcgc
    19441 agcgatgatc gaagtaatcc gaaggaatcc tcgagcctac tgcgtattgg taacacggga
    19501 tatcaacctt cagaacaaag ctgagttcgc cagagtccct tatgaggaac cttctgaacc
    19561 tcagagggct aagtaaggtg aagtcagctg atgtattgag aaacaactga ctttggcccc
    19621 tgcggttctg tgcatgtgta tgttcgcagt cttgctacgt ggccgtaact gggtgttgca
    19681 gctccatgtg ctccgtggtc tcgcccgaat gtccccagtc aacagatact tgcccgtagg
    19741 tgttgacctt ggtgtgcacc gggctgagga cgtggacata atcgtgtaac aagtcacaga
    19801 ctgctacgaa atactgatct tgcctttaga actgtactgc gttgttctta cacaagtcca
    19861 cagctttgcc aggcgtaaga agtttgtaga tgcagagcga tcattggtat ccggaaattg
    19921 aatagagttg tgagtttgtt aacgccattt cagaaaacca gatgcctagt gcgtagactg
    19981 ggtggcattt cggctgtgaa gttctgtaac ggtatatgtt tgggggctgt aatcgatgag
    20041 cataaacctt cgtggttcat atcgagcttt gcttggcaat gctattgagg cgctttctgc
    20101 agctgttgaa atatacaaca aaccaaagat aagctacaga gaggaatgtt cgataatact
    20161 cttgataaat gcgtgggagc tcctattgaa ggcactatta tccaagaaca agcaacgcat
    20221 ttactatccc aagaagcgga atcagcccta tcggacatat agcttgaagg atgcattgac
    20281 aaaagccgag gaatttttcc cggaaagcgt tgactatcat ccaacaagag acaatatctt
    20341 gctcctcaaa cggtacaggg atactacgat acatttctac aatgatgagt cacttcatgt
    20401 tccgctttat gctttggcac agacttcaat agttaacttc agagatgttg ttagtgaatc
    20461 gtttgaccgt gacattgcag aagagattac tttgtcgtta ttgccgttgt ccattgctcc
    20521 gccaattgat tcagtagagt tcttaagggc ttgcggtggt aatggaatgc aatcaagacg
    20581 aactcgtgaa tttacgcaaa gtatacgcaa cattgtgatc aaatctgaag aggcgggcta
    20641 tgatacaggg aggatactca ctgccttcaa cgttcaactt gtatcggtca agaaggtttc
    20701 gtctgcagac tttgtagttg gcgtagatgg tagtcaggta ggggaaggcc gacccgtcct
    20761 cgtccacaaa ctcagagatc caaacattac gcatccatac agagagtcag acataattac
    20821 aagcaaggca aacccgaata aagctggtct agagctcaca attggaggag cgaaactgac
    20881 ccaacatacg ttcagggcaa ttacatggaa atatgatata aggaatcagc ccaagtactg
    20941 ttggatggcc aagaacagac atcttgttct ctactcccgc gaatatgtca catttttgaa
    21001 acgcttgaca gaggatgatg tcaaacaggc gattgcagac tataagtcgt ggtgtaggag
    21061 ccggaaccat aggtaagcaa cctcagactg agactgaaag cattggtgaa tccgcatcag
    21121 ggatgcccat cagtgaccta cgggttcccg gcagagtctt ggcgagcagg gtagtgatca
    21181 tcacggccgt gggtaggccg cttcgaccgg cagctggtgc cgccgagcgc aagcaaattc
    21241 cctgagcgag gtgttcaccc ctatggtgct tgcccatagc cagtcgtgac atggcatgcg
    21301 attgttgaac aaatcacaca agttgcagag cgaggttgaa gcacaagaac gtgcaaagaa
    21361 gcgaactttc tatcaagaag agataacaac ctttcataca caatcttatc acagacctcc
    21421 caagctgcag ataatcgcca actatctggt tcggctagcc aaggttagtt aaagggtgat
    21481 tgcaatatga acgaagtgat ccgttcgttg ggcacgtaca ggtctcggcg aaacatgtct
    21541 aggcacgcta gaaccctgca aaaagcgtgc aaaagcataa ggaacgatcc attcttcgaa
    21601 gaatggcact ttgtgatcat ggatcgtgat tcaccgtata gaggcctctt ccaagagtgc
    21661 gttcttgggg ttgttgcctg ccctggttgg gcagatcgga gcaacgagag gctcgtgctt
    21721 atggcgcggc acgtggatgc ggacgagctt ggccagagct gccgtccccg gaaaatcgaa
    21781 tacgcaatca agacagttgt tcttcctccc ccggggtcat tgcccatagc tcttggcggg
    21841 agcgaagaca catatccccc atacaccgaa tccatccgcc tagctacggc ctttagggag
    21901 agggcctgcg aatgggaggc tgagttggcg cacagaatga cagcccctac attggactcg
    21961 tcttttggca tggcccaaca agggctatct tctatgtatg cagatcaaat gccagaagat
    22021 tatctgtccg agaagagccg cacgggcgag ccgctgatgg ttcaaaatac actggaatgg
    22081 ccccaagcgg ctagccaggg catacccgat agcgcgcctc tgcggcaaca ggtctcagat
    22141 atgccggaat atgattctgc agaaatgcag cctattagga tcgacgttcc cgaggctaca
    22201 acaataactc aatatgagac tcgcatctcg gaggttctca aaggcgctca gttcttgggc
    22261 cgcattgaaa tgccagagga gcagcttgaa gccttattgg gaatcatcgc atcccaactt
    22321 gatggagtca tgtatcccaa agcatgtgcg cgccgccttg cccagaacta ccctgccgct
    22381 gttgcgtacg ccctcgtcca agttgccgca gaagagtacg ccgagggtga agtgtggcca
    22441 agaatcgccg agcatttggg aggtctggaa accgcggtcg tgaacgagtt gggcatcagc
    22501 attgaaaggt gtc
//
LOCUS       DAOSAI010000004        20358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_1862_length_20358_cov_3.908831, whole genome shotgun sequence.
ACCESSION   DAOSAI010000004 DAOSAI010000000
VERSION     DAOSAI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 20358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20358
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1862_length_20358_cov_3.908831"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /gene="mnmG"
                     /locus_tag="PLM83_00300"
     CDS             complement(<1..1254)
                     /gene="mnmG"
                     /locus_tag="PLM83_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOK69888.1"
                     /translation="MDYDVIVIGLGHAGCEAALACARLGCSTLGLTVSMENIAQMSCN
                     PSLGGPAKAHLIREIDALGGEMGLAADETFIQLRMLNTSKGPAVRGLRAQIDKAEYQA
                     RMRRTLQRQPGLVLTQATVYDIIVRNGRVAGVSTEIGEISSRAVILATGTHLGGRVHM
                     GEMNYESGPDRQPAAVELGENLRRRGFAMYRFKTGTPARIDGRTVDYDATQIQLGEEL
                     PYGFSYLRRPYPREQIPCWQTWTNERTHEVIRENLDRAPMYTGAITGTGPRYCPAIET
                     KIVRFPDRPRHQVYVEPQGCGNEEMYLSGVSTSLPVDVQEKMIRTIRGLENVHIMKYG
                     YAIEYDCIDPTQLAPTLESLKIAGLYFAGQINGTSGYEEAAAQGLVAGINAALSVLRP
                     DERLLLDRSEAYIGVLIDDLVSRGTD"
     gene            complement(1325..2800)
                     /gene="mnmE"
                     /locus_tag="PLM83_00305"
     CDS             complement(1325..2800)
                     /gene="mnmE"
                     /locus_tag="PLM83_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOK69889.1"
                     /translation="MFREDTIAAIATPPGIGGIAVIRISGPEAKDVAQRVFVPARPGA
                     ASDGAAGLYPIRGWLCKYGHVHDASGELIDEAIMTWMPGPTSYTREDVAEISCHGGTA
                     VARAVLEATLKAGARIAQPGEFTRRAFLNGRISLEQAEAVLSMVNARTERARKAASSF
                     LAGSLGVKVREAEESIISLMTVIEASLDFPEEDIPDVAVQEIADESSRIAAMLEEACQ
                     EASMGRLLWEGASVAIVGRPNVGKSRLLNALLKEERAIVSELPGTTRDVVSEVANIRG
                     IPIRLIDTAGMRDSVDPVERIGVERAKIALADADVALAVVDGSEPLNMADAEIVSQTP
                     ESHTIVVVNKCDLANVMGDEDARRIAAGRPVLRVSALTNEGLDNLEEAIEALVLGDRG
                     AGIAEIGALAGQGLHGGQAADQRDGLETESEMLGMRREDCLRRAAQSLRDVACGVEEN
                     MPPDMLSIDLRSALDALGELTGETTREDIVDRIFAQFCVGK"
     gene            complement(2979..3749)
                     /gene="jag"
                     /locus_tag="PLM83_00310"
     CDS             complement(2979..3749)
                     /gene="jag"
                     /locus_tag="PLM83_00310"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOK69890.1"
                     /translation="MEPVEAVGKTVEEAVESGLSTLGLARSQVEIEVIDEGSKGLFGL
                     VGRRHARVVVRPLANIESKGEAYEGAMGAVESPVRAEEDLGGIDEDDDEWRGGSSFDE
                     DDAKSSGYVFLEELISLMGVDVDITETRSEDITLLEVEGRSLGMLIGRRGETLDAIQY
                     LVNLAANREARASGSSERERYVVDIGGYRKRREEVLENKALELAERVVQEQRDEVMEP
                     MSALERRVVHMALRDYDGVVTHSEGREPYRRIVISPKE"
     gene            complement(3750..4382)
                     /locus_tag="PLM83_00315"
     CDS             complement(3750..4382)
                     /locus_tag="PLM83_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOK69891.1"
                     /translation="MGITGVMQSILRFFAEGTGNLAWAIIIVTTLIRAALHPLQVKQV
                     RLMEQMKEITPKVQELEKKYKGQPEEYQRRVAELYRDNNVNPAVGCLSMLIPLPFFVI
                     FYRVLQDTAFIGGMPEAAKGFLWIKDFALPDPWALPILSAVTTWLSSRQTATDPSQKS
                     MAIIMPIFMGWIAMRMPAGAALYWVTNNLISVVQQWLIQRQLAAAKKGVS"
     gene            complement(4450..4668)
                     /gene="yidD"
                     /locus_tag="PLM83_00320"
     CDS             complement(4450..4668)
                     /gene="yidD"
                     /locus_tag="PLM83_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOK69892.1"
                     /translation="MWAVRIELGLIRFYRTCISPLKRYPCCRYVPTCSQYAEEAVKKY
                     GVIRGTWLAMKRLARCHPFSAGGYDPVK"
     gene            complement(4687..5064)
                     /gene="rnpA"
                     /locus_tag="PLM83_00325"
     CDS             complement(4687..5064)
                     /gene="rnpA"
                     /locus_tag="PLM83_00325"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOK69893.1"
                     /translation="MPTVSLRRTSDFQRAFREGKSAAGQYVVVYAVPNGLPEVRLGYP
                     VGKKLGGAVKRNRIKRLLREAARLAPNMPGMGYDMVLVPRRKIADPNVRCHNVIGDLE
                     KVLDRLSLETELGEPRSCGGSDV"
     gene            complement(5176..5310)
                     /gene="rpmH"
                     /locus_tag="PLM83_00330"
     CDS             complement(5176..5310)
                     /gene="rpmH"
                     /locus_tag="PLM83_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOK69894.1"
                     /translation="MKRTYQPKVRRRNRVHGYRKRMSTPAGRNVMRRRRAKGRKRLTV
                     "
     gene            7565..8806
                     /locus_tag="PLM83_00335"
     CDS             7565..8806
                     /locus_tag="PLM83_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK69895.1"
                     /translation="MALFKFLFGRMNGEKSQTEDSVAEVSAIAPESAPAYTLRIATYD
                     SMSILPRTIDLSSDNFASFVNEASSRVYAFCKEKGGSMPFVVLREAIENLIHAGFEDA
                     VITIMPDGNVVRISDHGPGIRAKSQAMMPGFTSATADDRKLIKGVGSGFPIMIESLES
                     LGGFLTVEDNLGSGLVVTLCSSPDARPGGVSPKPDRASQKTAGPSQPVRHDPAHMQPA
                     QIAQGFTEAAWSSRQDISGAYHPPTQGIFIGHPQDGDGHIFAEGPQGSGGVRPQRDPG
                     AEISQTSSAVYNESDSIDMENIADEGRATLSVEKQDSAADQSKKRRDEEPPSTSQSSD
                     MSDEALGALLSSRQKKVFLLIAEMGEIGPSVVTKELDISLSTAYRDLVTLEELGLVVS
                     LEGGKRKLTRRGVDFLSYVFR"
     gene            8857..10314
                     /gene="dnaA"
                     /locus_tag="PLM83_00340"
     CDS             8857..10314
                     /gene="dnaA"
                     /locus_tag="PLM83_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOK69896.1"
                     /translation="MDVLQVLESELSRPSFDAWLKTSVPVVVADDIVEVPVPHDFARD
                     WLEMRYAALLSETMSSMVGRKITVRFIARESSAEAENGDGAVSEDSYEMGNHANPATR
                     EPSPANALDANAASGMGVDEAAATSGTYAQTQAEAKAFDDFSTPALNPKYTFESFVVG
                     NSNRFAHAAALAVSEAPGKAYNPLFIYGGVGLGKTHLMQAIGHHVLEINPYAKVVYVP
                     CEQFTNELINSIRFETTIQFRERYRNVDVLMIDDIQFLAGKERTQEEFFHTFNTLYES
                     GKQIVLSSDRPPKEIATLEARLRSRFEWGLITDIGTPDLETRIAILKKKAVSEGLDVP
                     NDVLVYIAGQIHSNIRELEGALIRVTACAALTNREITTQLAAEVIQDMIPSSRPKQIS
                     VELIQRTVADYYHLEVSEMVSKTRVRTVALPRQIAMYLCRELTELSLPKIGEEFGGRD
                     HTTVIHACDKIQNELKKDSTMNANIKEIVSRLKSS"
     gene            10592..11728
                     /gene="dnaN"
                     /locus_tag="PLM83_00345"
     CDS             10592..11728
                     /gene="dnaN"
                     /locus_tag="PLM83_00345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOK69897.1"
                     /translation="MKVSCSKAALVSGVQTVYKAVSPKATIQALSGVLVETCTQGLRL
                     VAYDLDELGIETFVPVTVGTEGSTVIPAKYFSDISRKLPQSEVILEYREESQSVSISA
                     GRADFTVKTMPAAEFPALPEISEANSWSMPESELKTMIRRTIPAAAVDDARAFFKGIY
                     MLFEKDSLTMVATDSFRLAYRKTQASFDLEEPIDVIIPAKALLEVERNLNDTGEREVS
                     IAIEKNHAMFRIGPTTYVTRLLEGQFPNYKQVFPENIPAKIICDRLQLLDSVDRVSLM
                     CKDELSAIRMSYTGDEGMASGFLTITAATPEVGEGKEDIPVSMIGHAEVTVAMRARYL
                     RDVLRVLDGEEVCLGYTSYRHPIEVTDETDPAYTYLLMPVTAPV"
     gene            11742..11990
                     /locus_tag="PLM83_00350"
     CDS             11742..11990
                     /locus_tag="PLM83_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005399216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOK69898.1"
                     /translation="MKNMTLEIETEYIELDQALKLAALVGSGGEAKMAIHSGWAKVNG
                     AAEFRRGRKLRDGDVLEFADVVVTIVSGGSSGSGKEDV"
     gene            11987..13243
                     /gene="recF"
                     /locus_tag="PLM83_00355"
     CDS             11987..13243
                     /gene="recF"
                     /locus_tag="PLM83_00355"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOK69899.1"
                     /translation="MTSVWVRNIQLSGFRNYSKESVTFDPGLNLFLGHNAQGKTNLLE
                     AVYVASAGRSYRASSDSELVQKGSSYYRVAVDVQRKPAPVTLEVSYSTDGRKLVRLNG
                     TERIRAAELSSFLNVVLFTPDDLALVKGSPSVRRKFLDLEISQVSPAYRQLINLYSKI
                     VTQRNALLKQARERNWRSDSAAASRLLESWDEQLIETGSKITARRMQVVASLDELGRE
                     AHYRITGVPGALSVRYLPSVPLFADEQGSTSEATVDWIAERYRARLRQLRKVELIRGV
                     TMVGPHRDDISFQLDGADVATYGSQGQQRSTVLAVKLAEANFIRAHTGDMPVLLLDDV
                     LSELDETRRTNLLGEIVGDNQVLVTSVEPGCRDLMPRGAAVFSIVAGEVTPDDASHRG
                     CSGKEHGQDGIHRPDPGDIRHASVER"
     gene            13143..14102
                     /locus_tag="PLM83_00360"
     CDS             13143..14102
                     /locus_tag="PLM83_00360"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOK69900.1"
                     /translation="MMLPIGDVLGKSMDKMGYTARIRATSAMLVWNDEIAPSLAVASR
                     ATFVRGKTLYVTVEKPVWAQQLSMMKRGLVQRINASAGKDAIRDIRFRSGQVRAPLVW
                     GADSEAGLKFPSPSAQEEGVPDWKSMPADEDALSTAANASRPIGDPEVREKFSDLMIT
                     DAKWRAWSRQNLSSNAVKAADILKKEPWLSDSHLASMVPGASSDDLIRARDAAAQELR
                     REIASLVAVDRLESRFASGISEAPTGKSSCLHEDATDRGVDSLGRQGIVGDAKRLVRI
                     RLLVESLAMLVAGEPPDKIDEELVERVLGSEYADLVRYLSEEL"
     gene            14102..14398
                     /locus_tag="PLM83_00365"
     CDS             14102..14398
                     /locus_tag="PLM83_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOK69901.1"
                     /translation="MVYQSERSGLHGRYMYIGSDVLIDGRDVVAIFDMKSVSRSREMK
                     AFMESKKKAGKVIDISEGEPKAIVVTSEDVYLCPVSVGTLRNRISGPFDGEGDE"
     gene            14497..16425
                     /gene="gyrB"
                     /locus_tag="PLM83_00370"
     CDS             14497..16425
                     /gene="gyrB"
                     /locus_tag="PLM83_00370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOK69902.1"
                     /translation="MGHEAQNDNNNLAYNAAQIQVLEGLEGVRKRPAMYIGSTDSKGL
                     HHLVYEVVDNSIDEALAGYCDTISVTLGKDGSVTVEDNGRGIPVGIHPHEGKPALEVV
                     LTKLHAGGKFGGGGYRVSGGLHGVGLSVVNALSERLEVWVKLDGRLHYQGYTRGVPDA
                     PLRVIGEASGTGTTIKFVPDATIFETVTYSYESLASRLREFAFLSAGVKLILRDERGD
                     EAREAVFEYEGGIRSFVEYINRAKDPIHPDVVYVGKVLDKVTVEVAIQYNDGYNENML
                     CFANYVNTTEGGTHLSGFRSALTRSINDYARKSGLLKENDDNLTGDDTREGLTAVLSV
                     KLEDPQFEGQTKTKLGNSEIRGIVDSVLSEGLSTYFEENPATARAIVEKCVSAARARE
                     AARKARELTRRKSALEISSLPGKLADCTLRDPELCEIYIVEGDSAGGSAKSGRDRRFQ
                     AILPIRGKIINVEKARLDRILANNEVRAMVTAFGTGIGEDFDLSKLRYHKIVIMTDAD
                     VDGAHIRTLLLTFFFRFMTPLIENGHVYIAQPPLYMVKEGKKEHYVYSDRELDELMNA
                     LGKGNYPIQRYKGLGEMNPEQLWETTMNPQTRTMLRVTMEDAIAADQIFTTLMGDKVE
                     PRREFIETHAKFVTEIDI"
     gene            16437..18881
                     /gene="gyrA"
                     /locus_tag="PLM83_00375"
     CDS             16437..18881
                     /gene="gyrA"
                     /locus_tag="PLM83_00375"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOK69903.1"
                     /translation="MTELVDGKIVPIDIENEMRKSYLLYSMSVIVGRALPDVRDGLKP
                     IHRRILYAMHGLGLTADKPYRKSATVVGEVMGNYHPHGDAAIYDALVRMAQDFSMRNT
                     LVDGHGNFGSVDGDPPAAMRYTETRMTRLAAHMLADIEKDTVNFVPNFDGSKKEPEVL
                     PARFPQLLVNGSSGIAVGMATNIPPHNLVETIDGTIELIDNPDATIEDLMEHIKGPDF
                     PTGGLILGTQGIREAYHTGRGSIKVRARARIEPMSGGRHRIVVTEIPYQVNKARMIER
                     IAQLARDKRIEGITDLRDESDREGLRIVMELKRDANPNVVLNQLYKHTTMETTFGAIM
                     LVLVDNEPRVLNLKEMLHYYIEHQKEVVTRRTQYDLSKAEERAHILEGLRIALDHIDE
                     IISLIRASRTREIAREGLITKFGLSDRQAQAILEMRLQSLTGLEREKIEQEYEEMCAL
                     IAHLREILGNEQLLLGVIKDELIEIRDKFGDPRRSEITQDFSELEIEDLIAAEDVVVT
                     MSNQGYIKRLPINAYKAQRRGGKGVTGMATREEDFVEHLFITNTHDTILFFTNMGKVY
                     SLKGYEIPEAQRHARGTAVVNLIELDPGEAVTALMPVSKFDEDVYVFMATKNGTVKKT
                     ELSAFANIRRSGIIGISLAPDDELIAVRMTGGDDEILLVTKDGMSIRFRQDNVRAMGR
                     TAAGVRGINLDKGDAVVGMDIARDNADVLIITSAGYGKRTPVVEYRPQNRAGKGLKAC
                     RLTNKTGNIVGVKLVREENEMMLTTMAGYVIRFQISDVPRMGRLTQGVKVMSLDDGDQ
                     VISIARVSSKGEDDLE"
     gene            18906..19508
                     /locus_tag="PLM83_00380"
     CDS             18906..19508
                     /locus_tag="PLM83_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOK69904.1"
                     /translation="MATERIFIEPFGEAGDAAYRVYASTRELLFAGCGLALSNLILPS
                     AWEEAEILEPIAERSFAAGGTLMETRSAVSSASILNVVPGASPAERSFVLSSRDLDLL
                     LVDFLNELIYLFDSEGFWGYSFEPKFADPKSKDPAGSVDAEMRLAVRTSGVILPPSAL
                     EPEQPEDGILGIVPKGVSYHMLEAGQGKDGRWHAQFVIDA"
     gene            19620..>20358
                     /locus_tag="PLM83_00385"
     CDS             19620..>20358
                     /locus_tag="PLM83_00385"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOK69905.1"
                     /translation="MSKEWTGPLERIEECMWMIPKTYKPGMLADAVVIADEESLRTAC
                     EGEALEQLANSAFLPGICSPAMAMPDIHSGYGFPIGGVGATDLESGVITPGGIGFDIN
                     CGVRLVRTDLEAGDIAPKIKPVIDRLYRDIPSGLGTSGKISLSAQEVRKVLQEGAEWA
                     VKAGYGFEADLEHTEEGGSMEGADASAVSKKAVERGMPQLGTMGSGNHFLELQVVEEI
                     VNPEVAKVFGLFEGQVVMMIHSGSRGLG"
BASE COUNT         4823 a         5490 c         5425 g         4620 t
ORIGIN      
        1 atcagttccc ctcgagacca gatcgtcgat gagtacgcca atataggcct ctgatctatc
       61 gagcaagagc cgctcatcag ggcgaaggac actcaacgcc gcattgattc ccgccacaag
      121 tccctgcgcg gccgcctcct cgtaaccgga ggtcccattt atctgccctg cgaaatacaa
      181 tcccgctatc ttcagggact cgagcgtagg cgccagctga gtcgggtcta tgcagtcgta
      241 ctctatggcg taaccgtact tcatgatgtg cacattctcg agcccacgta ttgtcctgat
      301 catcttctcc tgaacatcga caggaaggct tgtcgacaca ccggacagat acatctcctc
      361 gtttccgcac ccctgaggtt ccacataaac ctgatgacga gggcggtcag ggaacctcac
      421 aatctttgtc tcaatcgcag ggcaatatcg cggccctgtc cccgtgatcg cgccggtata
      481 catgggcgca cgatcaagat tctccctgat cacttcgtgg gttctttcgt ttgtccacgt
      541 ttgccagcat gggatctgct ctctcggata gggccgtcgc agatacgaaa agccgtatgg
      601 gagttcctca cccaactgga tctgagtcgc atcgtaatcc actgtcctcc catctattct
      661 ggctggggtt cctgtcttga agcggtacat ggcgaatcct cgccgcctca ggttttctcc
      721 cagctcaacg gcggccggct gtctgtcggg gcctgactcg tagttcatct cccccatgtg
      781 gaccctgccc cccaggtgtg ttcctgtagc aaggatcact gcccgggacg aaatctcccc
      841 tatctcggtg ctcactccgg ctactcttcc attgcgcacg atgatgtcat acactgtagc
      901 ttgtgtgagc accagtccgg gctggcgctg aagcgttcgc ctcatcctcg cctgatactc
      961 ggccttgtct atttgcgctc tcaacccacg aacagcgggg ccttttgaag tattcaacat
     1021 ccgcagctgg ataaaggtct catctgcagc aagacccatt tctcccccca acgcgtctat
     1081 ctcccgaatg agatgggctt ttgcgggtcc gcctagtgag ggattgcacg acatctgcgc
     1141 tatgttctcc atgctcaccg tcaatccaag cgtgctacat cctaaccgag cgcatgccag
     1201 agccgcttca caacctgcat gccccagccc aatcactatg acatcgtaat ccatttccga
     1261 ttcacccccc gtctctcaca tgccgcctat cgcccatgcg gtcaggcaca accgtattcg
     1321 actcctactt tccgacgcaa aactgggcaa atatcctatc cacaatatcc tctctcgttg
     1381 tttcgccagt aagctccccc agcgcatcca gggccgacct caaatcaatc gacagcatgt
     1441 cgggcggcat gttctcctcc acgccgcagg caacatctct gagggattgt gccgcgcgcc
     1501 gcaagcaatc ttcgcgtctc atgccgagca tttcggattc tgtttcgagc ccatcacgtt
     1561 gatctgcggc ttgaccgcca tgcagccctt gtccggccaa cgcgccaatc tcagctatac
     1621 ccgcaccccg atccccaagc accaatgcct cgatggcctc ctccaagttg tcaagccctt
     1681 cgttagtaag cgcggaaacc ctgaggacag gacgtccggc agcgattcgg cgcgcatcct
     1741 catcgcccat gacgttagcc aggtcacact tatttacgac gacgatggta tgcgattccg
     1801 gtgtctgcga cacgatctcc gcgtcggcca tgttgagcgg ctcagacccg tccacaacag
     1861 caagtgccac gtcggcatct gccagagcta tcttagccct ttcaacaccg attcgctcca
     1921 ccggatcgac cgaatcgcgc attccggctg tgtctatcaa ccttataggg atcccgcgga
     1981 tattggcaac ttcagacacc acatccctcg ttgtccccgg caattccgac acaatagccc
     2041 gctcctcctt gagcagggcg ttgagaagcc tggatttccc gacattaggc cttccaacta
     2101 tggccacaga ggccccttcc caaagcagac gtcccataga tgcttcctgg cacgcctcct
     2161 ccagcatggc agcgatccgc gacgattcat ccgcgatctc ctgcaccgca acgtcgggta
     2221 tatcctcctc cggaaaatcg agcgatgcct caatcaccgt catcagggaa atgatgctct
     2281 cttcggcttc acgcactttc acgccaaggg atccggcgag aaatgaggag gccgctttcc
     2341 tagccctctc tgttcgggca tttaccatgg aaagcaccgc ttcagcctgc tcaaggctga
     2401 tccgcccgtt cagaaacgcc cgccgcgtga actcgcctgg ttgagcaatg cgcgcacctg
     2461 ccttcagagt agcttcgagg accgcgcgcg ccacagcagt tcctccgtgg caacttatct
     2521 cagccacatc ttcgcgagta taagatgtgg gcccgggcat ccaggtcatt atcgcttcgt
     2581 ctattagctc ccctgaggcg tcatgcacgt gtccgtactt gcataaccac cctcggatag
     2641 gatacagccc agccgcgccg tctgacgccg cacctggccg agcaggcaca aaaacacgct
     2701 gggccacatc tttcgcttcg ggaccagata tcctgattac ggctatcccc ccaattccgg
     2761 gaggtgtggc aatagccgct atcgtatcct ctctgaacat ctgagcttcc cccttccttc
     2821 agactctaac cacacgcaca atctccgtga gtgtcaacca agtcgatcgg catccccaaa
     2881 tcccgcagcg ggcttcctca tctagagcat aaaagaaagt gcgcgtcatt aaaacacgca
     2941 ctcctattca tcgtcaccct ctatacgctc gatccttttc actctttggg ggatattact
     3001 attcgcctat atggctcccg gccttccgag tgcgtaacga cgccatcgta gtctctgagg
     3061 gccatgtgga ctacacgtcg ctcaagggca gacatcggct ccatgacttc gtccctttgc
     3121 tcctgcacta ccctttctgc aagctccaaa gccttgttct cgagaacctc ttctcgcctc
     3181 ttgcgatacc cgccgatatc gactacgtat ctctccctct ccgagcttcc ggacgcccga
     3241 gcctccctgt tggctgccaa gtttaccaga tattgtattg cgtcgagcgt ctctcctcgc
     3301 ctgccaatga gcatgccaag agatctgccc tccacttcca gcagggtaat atcttcagac
     3361 cgggtctccg tgatgtcaac atctacgccc atcagggaga tcagctcctc caagaacaca
     3421 tatccactgc ttttagcatc gtcctcgtcg aaactggacc cgcctctcca ttcatcgtca
     3481 tcttcgtcga tgccgcccaa atcctcttca gccctcacgg gagactcgac tgcgcccatg
     3541 gcgccttcat aagcctctcc cttgctctct atgttggcaa gtgggcgcac aacgaccctt
     3601 gcgtgtctcc ttccgacaag cccaaacaat cccttagatc cttcatctat gacttctatt
     3661 tcaacctgac ttcttgccaa acccagagtg gacaaacccg actcaactgc ctcttcgaca
     3721 gtcttgccta ctgcttcgac aggctccatc tagctgactc ccttcttggc cgccgccagt
     3781 tgacgctgga tcaaccactg ctggaccaca ctgatcagat tgttcgtgac ccaatacagc
     3841 gccgcgccgg ccggcattct catagcaatc cagcccataa atatgggcat gattatcgcc
     3901 atggacttct gtgacggatc cgtagccgtt tgcctgctcg aaagccatgt tgttacagct
     3961 gataaaatag gaagggccca tgggtccggg agggcgaaat cttttatcca taaaaatccc
     4021 ttcgcagcct ccggcattcc gccaatgaac gcggtgtctt gcaaaacgcg gtagaatatt
     4081 acaaaaaacg ggagcggaat aagcatgctg agacatccca ccgccgggtt tacattattg
     4141 tcacggtaca attccgccac ccgacgctgg tattcctcag gctgcccctt gtacttcttc
     4201 tcgagctctt gcactttcgg cgtaatctct ttcatctgct ccattagcct gacctgctta
     4261 acctgcagag gatggagcgc agctcttatt aaagtcgtta ctatgatgat tgcccatgca
     4321 aggttgcctg ttccttctgc gaagaatcgc agaatgctct gcatcactcc agtgatgccc
     4381 actggcggat ccccctttcg cacttaggct tggctttctg ccctagctat cgccatttgt
     4441 tcatcacgcc tacttgacag gatcgtatcc cccagcgctg aacgggtggc accttgccag
     4501 tctcttcata gccagccatg tgcctcgaat aacaccgtac ttcttgaccg cttcttcagc
     4561 atactgcgaa catgttggaa catagcgaca acacggatac cttttaagcg gagatatgca
     4621 tgtcctgtaa aaacgaatca accctaactc gatccggact gcccacattt gctgcccctc
     4681 caaccctcat acatccgatc cgccacagct tctcggctct cctaactcag tttcgagcga
     4741 tagtctgtca agaaccttct ccaaatcgcc aataacatta tggcagcgca cgttcggatc
     4801 tgcaatcttt cgcctcggca cgaggaccat gtcatatccc atgccaggca tgttgggcgc
     4861 gaggcgcgca gcttccctca acagcctctt tatacgatta cgcttgacag cgccgcccag
     4921 tttctttccc acagggtacc ccaggcgaac ttcaggcaag ccattaggca cagcgtacac
     4981 cactacatac tgtcctgcgg cgctcttgcc ttcgcggaaa gctctttgaa aatctgaagt
     5041 tcgacgcaaa ctcactgttg gcattggcgg gcctcctaac agcccaatga agacagaaca
     5101 ccctgcattt aatagggagt gtccactaaa ggaaaagccg tccgacgacg gccagaaacc
     5161 agcgacgccc tgcgcctaca ccgtaagtcg cttgcgcccc ttcgccctgc ggcgcctcat
     5221 cacgttgcgg cccgctggcg tgctcatgcg cttcctatat ccatggactc ggtttcgacg
     5281 cctaacttta ggctgatatg tccttttcat ctcaacttat cctccctgca agctcaccgg
     5341 cagcataccg cgtaaattat atgctaggcc catattggtg tcaaggaatc gcaaggacgc
     5401 gtgatttggg ttgtagcgga tttcccgcgc gggcgagtta gacagtttag caagagcagc
     5461 ttcctcccta gcaatgaagg agcatcattt gccgaaatag gtgtcagaag gcggtttttg
     5521 gctccccctg ggggaaaagg ggcaccttca cgtaaccgca ctcccatctc attgttcagt
     5581 ttgtcaaagg ctgcttttat acagcctgta cagggctacg caatctgtcc ccctgtttgg
     5641 tttttatcct gcccaaagcc atcaaccttc ttgatgcttc atcaggttgc acaaaagtcg
     5701 tgtatgccga cggcgagggg gcttcctcca cgcccagcgt aatgtcgagg ccgcaaacct
     5761 ggcgtagctg tcattttcta attagccccg acaaaggccc tcggtactct tacattaaaa
     5821 gatgatgcct tccattgccc cgtacttttt ctagcgcttg catcaactct tcatcaggca
     5881 ggtagtctat aacaagcttc aattgtccca aatcgcccaa tttctcaaca acttcattct
     5941 agcgaaacaa ctgcttttgt ggttctgtcc acgttgtttg gactatttgc aacattttac
     6001 actttgggcc ggttgccctg tttttcgtgc tgcaaaacgg gcttttccca tggaggaaac
     6061 ggattgtcag ccgtccttca aatacaagat ggctcgccaa ccggaacaga tctttgcgag
     6121 tttcgcattc ctttcaatgt gatccatttt cagttgttgt gccatctccg tatctgcatt
     6181 tgcttttctt cgggcctcag ccgccccaaa cccccaagtg ccttttgagc gctgctcaca
     6241 ggagtccctc aaacactttc agaaccatga agatcctttt gcagttctta tgcacacccg
     6301 caccgattcc tgccgctcat ccatctagcc ttgaattcgg caatccttgc cttccacatc
     6361 tattcacctt tcacctttac tctttgacat agataaatct ccccaagctt gtctcctcga
     6421 ggaggattat tgcacactcc agggcatcaa cccaggcggg cactgtttga cgattacccc
     6481 aaagcctgtc ttgcctcata tccctttccc cgtgatcttt gctcatctgc gcttttcaac
     6541 actcagccca tcttagtgct gcgcaccttc catcacgcag ctcattgtat cttctctcaa
     6601 tctcggagac aaagtcggcc actagccacg atcctaccta ttctattgca gcctcactct
     6661 ttgagttctc cagtttgtca cattctgata agtcgcatct gcttctgtcc attccaaaaa
     6721 accgacaatt cacatgctgt ggacaactcg caagaagcat ttcggctcaa ttggcaacca
     6781 aagcgctcac tcttagcaat agattctcgt ggatcttgca aacatgcatc ccgacttatc
     6841 aagcctcgat cgccggtacc tctttgtgga tcacccgcga tgatcagttt catcccatta
     6901 tcgctgcaaa cccgcgccaa aggatccgct gtctcacgcc atatattgcc gggcattgag
     6961 gcgttgtaat agtgaaaagc tgtgttgctc cctttgatct cttttcgctc tgttatgata
     7021 gtgtgtacaa acataccgac ttttcgctcg caaattcata caccaatgat tatcgccagt
     7081 tatgcgccct tttatgcaca gtctgtggat atccctgtgg ataacctgtg tgaagttcgt
     7141 ggatttccca caaccgccga tattcgggca aaaactttcc caaaaccatc tgtggataat
     7201 tgtgaaagcc gcctttgact ggcgaaaagc cagctaaaac atatgttcgc cccgaatctg
     7261 ctggcggtag gatatccaca ggtctctgtg gaatacttgt ggatgatatg ttataaacaa
     7321 tatgcggact ccgctttccg acgacttttc aacagctgtg gaaaacgttg tggataactt
     7381 tatggtgaaa acgtgatatt tcgagaatct tgtaaacccg actagcagca ttcgagcaat
     7441 agtcaataat tgtcgaacaa ttccgggcca ttttagctgg cgctaaagtt atccacattt
     7501 tcatgttgat aacctgtgga tgaaatagtg gagcggagag tgtcgaagcc aggaggctat
     7561 gcaggtggct ttgttcaaat tcctatttgg caggatgaat ggagagaaaa gccagacaga
     7621 ggactcggtc gccgaagtat cggcaatagc gcccgagtcc gctccggcgt acacattgcg
     7681 catagcaact tatgattcaa tgtcgattct ccctcgcaca attgatctct catccgataa
     7741 ctttgcgtcc tttgtgaatg aagcatcatc tagggtgtac gccttctgca aagaaaaagg
     7801 gggatccatg ccctttgttg ttctgaggga agccatcgaa aacctgatac acgccggatt
     7861 cgaggatgca gtaattacga tcatgcccga tggcaatgtt gtgcgcatat cagatcatgg
     7921 acctggcata cgcgccaaga gccaggctat gatgcccggt ttcacctcag cgacggccga
     7981 cgacaggaag ttgataaagg gggtgggatc tggtttcccg ataatgattg aatctttaga
     8041 gtccttagga ggtttcctca ctgtcgagga caatctgggt tcaggtctcg tggtcacgct
     8101 atgttcctct cccgacgcac gtcctggcgg cgtgtcgccg aaacctgaca gagcttcgca
     8161 gaagactgct ggtccatccc agcctgtacg gcatgatccc gcccatatgc aaccagccca
     8221 gattgcacaa gggtttacag aagcggcgtg gagctcgcgc caggacattt ccggcgccta
     8281 tcacccgcct actcagggca tctttattgg ccatcctcaa gacggagatg gacacatctt
     8341 cgccgagggg cctcaggggt cagggggcgt taggccacaa cgggatccgg gggccgagat
     8401 ttcacagaca tcttcggccg tctacaacga atccgactcc attgatatgg aaaacatagc
     8461 tgacgaaggc agagccactt tatccgtcga gaagcaggat tcggcggcag atcagtcgaa
     8521 gaaaaggcga gatgaggaac ccccatccac atctcaatcc tctgatatgt cagatgaggc
     8581 tctcggcgcc ttgctctcct cacgccagaa gaaggtgttc ttgcttattg cagagatggg
     8641 ggagataggg ccttcggttg tcacaaaaga gcttgacata agccttagca cggcatatcg
     8701 cgatctagtg accctagagg aattgggact cgtagtcagc ctcgaaggag gcaagcgaaa
     8761 gcttacgagg cggggtgttg acttcctcag ctatgtcttt aggtgatggt ccaaatgagg
     8821 ggtgacaaag tgtcccaaga gataaatgca atttggatgg atgttcttca ggtcctcgaa
     8881 agcgagctgt cgaggccaag ttttgacgca tggctcaaga ccagtgtccc cgttgtcgtc
     8941 gctgacgaca tcgtagaagt gccagttccg cacgactttg cccgtgactg gctggaaatg
     9001 cgatacgcag cattgctgtc agaaaccatg tcatccatgg tgggccggaa aataacggtg
     9061 aggtttattg ctagggaaag ttcggctgag gcagaaaacg gagatggagc cgtaagcgaa
     9121 gattcttacg agatgggcaa tcacgcaaac cctgcgaccc gagagccgtc gcccgctaac
     9181 gcgttggacg caaatgccgc atcaggcatg ggggtcgacg aagcggcagc gacttctgga
     9241 acatacgccc aaacgcaggc tgaagcgaaa gccttcgatg acttctctac accggcgctc
     9301 aatccgaagt atactttcga gtcttttgta gtgggcaact ccaacaggtt cgcccacgcg
     9361 gctgcgcttg ccgtttccga agcgcctgga aaggcctaca acccgctctt catatacggc
     9421 ggagtgggat tggggaagac ccacttgatg caggccatag gccatcatgt gttggagatc
     9481 aacccttatg ccaaggtagt atacgttcct tgcgagcaat tcacaaatga gctaataaac
     9541 tcgattcgct ttgaaacgac tattcagttt cgcgagcgat atcgcaacgt ggatgtcctg
     9601 atgatagatg atattcagtt tctcgccgga aaagaacgca ctcaggaaga gttcttccat
     9661 accttcaaca ctctctacga gtcgggaaaa cagatagtgc tctcgtctga tcgacccccg
     9721 aaagaaattg ccacgctcga agcaaggctg cgatcgcgat ttgagtgggg cctgattacg
     9781 gatataggca ctcccgacct tgagacaagg atcgccattc tgaagaagaa agctgtgtcg
     9841 gagggacttg acgttcccaa cgacgtgctg gtgtacatag cgggccagat acattcgaac
     9901 atacgtgagc tggagggagc tctcatcaga gtaacggcct gtgccgccct gactaatcgc
     9961 gagataacta cacagcttgc cgcagaggta atccaggaca tgataccatc ctcgcggcca
    10021 aagcagatat cggtggagct aatacagcga acagtggcag actactacca cctggaagta
    10081 tcggaaatgg tgtcaaagac gagggtccgc actgtagcct tgccacggca gatcgccatg
    10141 tatctttgcc gtgagctcac agagctttcg ctgccgaaga taggcgagga attcggtgga
    10201 cgggatcaca cgactgtgat ccacgcctgt gacaagattc aaaacgagct aaaaaaggac
    10261 tcaaccatga acgcgaacat caaggaaata gtttccagat tgaagagcag ttgagctgtt
    10321 gattatgtag ttaacaacat gtgaaaaggc tgtggaaaga aaaaacccct gtggataact
    10381 gatgataact cgccatctgc tccacaagta atcaacaatc tgtctacagt gacactggga
    10441 acagacgggc agcataaccc atgaaattcg acacttatcc agatatccac aggcacgtat
    10501 actactacta cggtaaatgt acatatgtac tcatagtagt acttatatat gcatgtgcct
    10561 ggaaatcagg tcagtgggga ggtatagcaa ggtgaaggta tcatgctcca aggcagcttt
    10621 ggtcagcgga gttcagaccg tgtacaaggc ggtctctcct aaggctacca tccaagcgct
    10681 ttcaggagtg cttgtggaaa cttgcacaca aggcctgagg ttggtagcat acgacctgga
    10741 cgaactgggc atcgagacat ttgttccagt tactgttgga acagaggggt cgacagtgat
    10801 ccctgcaaag tatttctccg atatctcgag aaagcttccg cagtcagaag tcattcttga
    10861 gtatcgggag gagagccaat ctgtgagcat atccgccgga agagcggact ttaccgtgaa
    10921 aaccatgccg gctgcggagt ttcccgcatt gccggagatt tcggaagcaa atagctggtc
    10981 tatgccagaa agcgagctca agaccatgat caggcgcacc attcctgcgg cggcagtcga
    11041 cgatgccagg gcttttttta agggcatcta catgctattc gaaaaggact cgctgaccat
    11101 ggtggccacc gactcattta ggcttgcata caggaaaacg caggcatcgt tcgatcttga
    11161 agagccaatt gacgtgataa tcccggccaa ggcccttctc gaagtggaga gaaacctcaa
    11221 tgacaccggg gagagggaag tctcgatagc catcgagaaa aaccatgcaa tgttccggat
    11281 agggcccacg acttatgtga cacgactatt ggaaggacaa ttccccaatt acaagcaggt
    11341 tttcccggag aatataccgg caaagataat ctgtgatcgc ctgcagcttt tggacagtgt
    11401 agacagggta tcgctgatgt gcaaggatga attgagcgcc atcaggatgt cgtataccgg
    11461 tgatgaggga atggcctcgg gctttttgac aatcaccgct gccacacccg aggtcggaga
    11521 gggcaaagaa gacatacctg tgtccatgat cggccacgca gaggtcaccg tggccatgcg
    11581 cgcaagatac ctgagggacg tgctcagggt gctcgacggg gaagaggtgt gcttaggcta
    11641 caccagctac aggcatccaa tagaggttac ggacgagacg gatcctgcgt acacttatct
    11701 tctgatgcct gtaacggcgc ctgtgtgatg atatgggacg catgaaaaac atgactttgg
    11761 aaatagagac tgaatatata gagctcgacc aggcgctcaa acttgctgcg ctggtcggat
    11821 cgggcggaga ggccaagatg gccatccatt cgggctgggc gaaggtgaat ggagccgctg
    11881 agttcagacg cggaaggaaa cttagggacg gcgacgtcct cgagttcgca gatgtagtgg
    11941 ttaccatcgt atctggcggc agctctgggt ctggcaagga ggatgtgtga caagcgtgtg
    12001 ggtgaggaat atccagttga gtggcttccg aaactacagc aaagagtcgg tgactttcga
    12061 ccctgggctc aacctgtttt tggggcacaa tgcacaagga aagactaacc ttctggaggc
    12121 tgtgtacgtt gcctctgcgg gccgctcata tcgcgcctca tccgattccg aacttgtcca
    12181 aaaagggagt tcgtattata gggtcgccgt agacgtccag cgcaagcccg cccctgtgac
    12241 gttggaagtc agttattcca cagatggaag aaagctcgtg aggctcaacg gcacggaaag
    12301 gattcgcgcg gcggagctgt catcttttct caacgtggtg ctttttaccc ccgacgacct
    12361 tgcgctggtc aaaggaagcc cttctgtgag gcggaagttt ctggatctgg agatatccca
    12421 ggtgagcccc gcttataggc aattgatcaa cctttactcc aagatcgtta cgcagagaaa
    12481 tgctctgctg aagcaggcta gggaaagaaa ttggcggagc gattcggccg ccgcgagcag
    12541 gcttctcgaa tcatgggacg agcagttgat cgagaccgga tcgaagataa cagcccgtcg
    12601 aatgcaagtt gtagccagcc ttgacgagct ggggcgtgag gcccactaca ggattacggg
    12661 agttcctggg gcgctttccg tgagatactt gccttcggta cctctttttg cggacgagca
    12721 aggttcaaca agtgaggcga cggtcgactg gattgccgaa agatatcgcg cgcgcctgcg
    12781 acaattgcgc aaggtggagc tgatcagagg ggtaaccatg gtcggtccac accgtgatga
    12841 catcagtttc cagcttgatg gcgcagacgt agctacctat gggtcccaag ggcaacaaag
    12901 gtcgacagtg cttgcggtca agcttgccga ggcaaatttc ataagagcgc atactgggga
    12961 tatgccagtg cttcttctag acgatgttct ctcagaattg gatgagactc gcagaactaa
    13021 tcttttggga gagatagtag gggacaatca agttcttgtt accagcgtgg agccagggtg
    13081 tagggatttg atgccgcgcg gtgccgcggt attctccatc gtagccgggg aggtgacgcc
    13141 cgatgatgct tcccatcggg gatgttctgg gaaagagcat ggacaagatg ggatacaccg
    13201 cccggatccg ggcgacatcc gccatgctag tgtggaacga tgaaatagcc ccatcattag
    13261 cggttgcatc gagggccact ttcgttaggg gcaagacgct gtatgtgacg gttgaaaagc
    13321 ccgtgtgggc tcaacagctt tccatgatga aaagagggct tgtgcagcgc attaatgcct
    13381 ccgccggaaa ggatgccatc cgcgatataa ggtttcgtag cggccaagtt cgcgcaccgc
    13441 tcgtgtgggg agcggattcc gaggccggcc tgaagttccc ttccccatct gctcaggaag
    13501 aaggggtgcc tgactggaaa agtatgcctg ctgatgaaga cgcgctctcc accgctgcaa
    13561 atgcttccag gccgattggg gatccggaag taagggagaa gttcagtgat ctgatgatca
    13621 ctgatgcaaa atggcgggcg tggagcaggc agaacctgtc ttcgaatgcc gtcaaggcgg
    13681 cagacatatt gaagaaagag ccatggctga gcgactcgca tctagcttcg atggttccgg
    13741 gggcatcaag cgatgatttg attcgcgcaa gagatgctgc tgcacaggaa ctgagaagag
    13801 agatcgcatc gttggtcgcc gtcgatcggc tcgaatctcg gttcgcctca ggcatcagcg
    13861 aggcgccgac tggcaaatct tcctgcttgc atgaggacgc tacagacaga ggggttgatt
    13921 ctcttggacg gcaaggaatc gtcggggacg cgaagcgact tgtgaggata agattgctcg
    13981 tggagtctct tgcgatgctt gttgccgggg aacctcccga caagatcgat gaggaattag
    14041 tggaacgagt cttggggtct gaatacgcag atttggtgcg ttatctttcg gaggagctct
    14101 gatggtttat caaagtgaga gatcgggcct ccacggcaga tacatgtata tcgggtccga
    14161 cgtattgatc gatggacgcg atgtagtggc cattttcgac atgaaaagcg taagtcggtc
    14221 ccgcgagatg aaagctttca tggaaagtaa gaaaaaggca gggaaggtga tcgacatctc
    14281 tgaaggtgaa cctaaggcaa ttgtggtaac ctctgaagat gtctatctat gccctgtatc
    14341 cgtaggcact ttgcgcaaca gaatcagcgg cccgtttgat ggcgagggcg acgaataaat
    14401 ctgtttgatg tataattagc ttggtgtgcg ccgtggtgcg tattagcaat gccgcggcca
    14461 tttttcggtt tgtgtttgct tttgggaggt atgttcttgg gacacgaggc tcaaaacgat
    14521 aacaataact tggcatataa tgcagcccag attcaggtct tggagggctt ggaaggggtt
    14581 cgcaagcgtc ctgccatgta catcggcagc accgattcca aaggcctcca ccatctcgtt
    14641 tatgaggtgg tcgacaacag catcgacgag gctcttgctg ggtattgtga cacgatatct
    14701 gtcacccttg gcaaggacgg ttcggtaacg gttgaggaca acgggcgcgg aatccctgtc
    14761 ggcattcacc cccatgaagg caagccagcg ctcgaagtcg tgctcaccaa attgcacgcc
    14821 ggcggaaagt tcgggggcgg cggctaccgc gtgtccggcg gtcttcacgg cgtaggcttg
    14881 tcagttgtga acgccctttc ggagcggttg gaagtctggg ttaaactcga cggccgtcta
    14941 cactatcagg gctacacgcg cggagtgcct gatgcgcccc ttcgcgtgat aggcgaggct
    15001 tcaggcacag gaacgacaat caagttcgtc cccgatgcga cgattttcga aacggtaact
    15061 tacagctatg aaagcctagc gtcgaggttg agggaattcg cctttctcag tgcaggggta
    15121 aagctcattc tcagagatga acggggcgac gaggccaggg aggctgtctt cgaatacgaa
    15181 ggtggaatcc gttcttttgt ggagtatata aacagggcca aagatccaat ccatccagat
    15241 gtcgtatacg tcggcaaagt cctcgataag gtcactgtgg aagttgccat acaatacaat
    15301 gacggctaca acgagaacat gctctgtttt gcaaattacg tcaatacgac ggagggcggc
    15361 actcatcttt ccggcttcag atcggcgctg acaaggtcga taaatgatta cgctcgcaag
    15421 agcggactcc ttaaggagaa tgacgacaat cttacaggag atgacacgcg agagggactg
    15481 actgcggttc tcagcgtgaa gctggaggat ccccagtttg agggacagac aaaaacgaag
    15541 cttggaaaca gcgagatccg gggtatagtc gattcggtgt tgtcggaggg gctctcgaca
    15601 tacttcgaag agaacccagc cactgcccgc gcaatagtgg aaaaatgtgt ttcggcagcc
    15661 cgcgcgcgcg aagcggcgcg gaaagcgagg gaactgacgc ggcgcaaatc ggcgctggag
    15721 atctcgtctc tccccggcaa gctagcagat tgcacgctga gggatccgga gctgtgcgag
    15781 atctacatcg ttgagggaga ctcggcaggc ggctccgcaa aatcagggcg tgaccgcaga
    15841 ttccaagcaa tattgcccat caggggcaag atcataaacg tggagaaagc ccgactcgac
    15901 aggatacttg ccaacaacga ggtaagggca atggtgacgg cgttcgggac gggaataggc
    15961 gaagatttcg atctgtcgaa actccgttac cacaagatcg tgataatgac agacgcggac
    16021 gtggatgggg ctcacatcag gacgcttttg ctgacgttct ttttccggtt catgacgccc
    16081 ttgatcgaga atgggcatgt atacatcgcc cagcctccac tctatatggt aaaagaaggc
    16141 aagaaagagc attatgtcta cagcgaccgc gaactcgatg agctcatgaa tgctctgggc
    16201 aaaggcaact acccgatcca gaggtataag ggccttggag agatgaaccc agagcaattg
    16261 tgggagacca ccatgaaccc gcagaccaga acgatgctcc gggtgaccat ggaggacgcc
    16321 atcgcggccg atcagatatt cactactctc atgggcgaca aggtggaacc gaggcgcgaa
    16381 ttcatagaga ctcacgccaa gttcgttaca gagattgaca tttgaggtgt tggggcttga
    16441 cagaactagt tgacggaaaa atagtcccga tcgacataga aaatgagatg cgcaaatcct
    16501 acttgctcta ctcaatgagc gtcatagttg gacgagcgct tcccgacgtg cgcgacgggc
    16561 tgaagccgat ccatcgcagg atactctatg ccatgcacgg tcttggcctc acagctgaca
    16621 agccttacag gaaatcagct actgtggtcg gcgaagtcat gggcaattac catccacacg
    16681 gcgacgcagc catctacgat gccctcgtga ggatggccca ggatttctca atgaggaaca
    16741 cactggtcga cggccatggc aacttcggct ccgttgacgg ggatcccccg gcggccatga
    16801 ggtacaccga gacgaggatg acacgtctgg cggcgcacat gctcgcagac atagagaaag
    16861 acacggtgaa cttcgttccc aacttcgacg gatcgaaaaa ggaaccggag gttcttcctg
    16921 cacggttccc gcagctcttg gtgaacggct catcaggcat tgccgtgggg atggctacga
    16981 atattccccc gcacaacctg gttgagacga tagatgggac gatcgaattg atcgacaacc
    17041 ccgatgccac aatagaggac ctcatggaac acataaaggg tcctgatttt cccacaggag
    17101 ggcttatcct cgggacccag ggaatccgcg aggcttacca cacgggccga ggaagcataa
    17161 aggtgagggc tcgggcaaga atcgagccta tgagcggcgg caggcacagg attgtcgtaa
    17221 ctgagattcc ctaccaggtt aacaaggcta ggatgatcga gcgcattgcc cagcttgcac
    17281 gcgacaagcg catagagggc atcacagacc tgcgcgacga atcggacagg gaagggctcc
    17341 gcatagtgat ggaactgaag cgggatgcca acccgaacgt ggttttaaac cagctttaca
    17401 agcataccac catggagacg actttcggcg ccatcatgct ggtgcttgtc gacaacgaac
    17461 cgcgggtcct caatctcaag gagatgctgc actactacat tgagcatcag aaggaagtag
    17521 taacaagaag gacccagtat gatctttcaa aggctgaaga gagggcgcac atactcgaag
    17581 gcttgaggat tgcgctggac cacatcgatg agataatttc gttgattcgg gcttcccgga
    17641 cgagagaaat agccagagag ggcttgatta cgaaattcgg gctttctgac agacaggccc
    17701 aggctatctt ggagatgcgc ctccagagcc taaccggctt ggagcgtgag aagatagagc
    17761 aggaatacga ggaaatgtgc gcgctcatag cccatttgcg cgagatccta ggaaacgagc
    17821 agcttctcct cggagtgatc aaggatgagc tgatcgagat ccgcgataag tttggcgatc
    17881 cgcgccgctc tgagatcacc caagacttct cggagctgga gatcgaagat ctcatagcgg
    17941 ccgaggatgt tgtagtgaca atgagcaacc agggctacat caagcgcctg cccatcaatg
    18001 cgtacaaggc gcagcgccgt gggggcaagg gcgtcaccgg gatggccacg cgcgaggagg
    18061 atttcgtgga gcacctcttt atcacgaaca cccatgacac aatcttgttt ttcacgaaca
    18121 tgggcaaggt ttacagcctg aaagggtatg agatacccga ggctcagcgc catgcgcggg
    18181 gcaccgctgt ggtcaatttg atagagcttg accccggaga agccgttaca gcattgatgc
    18241 cggtgtccaa gttcgacgaa gacgtctacg ttttcatggc cacaaaaaac ggtactgtaa
    18301 agaagaccga gctcagcgcc tttgcaaata tacggaggtc cggcataatc ggaatctcgc
    18361 ttgcacctga tgacgagctt atcgctgtga ggatgactgg cggggatgat gagatactcc
    18421 ttgttaccaa ggacggcatg tccatcaggt tccgccagga caatgtgagg gcgatgggcc
    18481 gaacggcagc aggagttcgc gggatcaatc ttgataaggg ggacgcggtt gtgggcatgg
    18541 acattgcccg cgacaacgca gacgtcttga taataaccag tgcgggctat ggaaagcgga
    18601 ctcctgtggt tgagtaccgt ccccagaatc gcgcgggaaa gggtctcaag gcttgccgcc
    18661 tcacaaacaa gacgggcaac atagtcggcg tcaagctggt gcgagaggaa aatgagatga
    18721 tgctaactac catggcgggc tacgtcatcc ggttccagat atcggatgtg cccaggatgg
    18781 gaaggctcac tcagggcgtc aaggtgatga gtcttgacga tggcgaccag gtcatctcga
    18841 tcgcgcgggt ctcttccaag ggtgaggacg acctggaata ggactgaata gtggtgtgat
    18901 ttcacatggc gacagagcgc atcttcatcg agccattcgg tgaggcgggc gatgccgcct
    18961 accgggtata tgcaagcacg cgtgagctcc tttttgccgg atgcgggctt gccctttcaa
    19021 atctgatact gccttctgca tgggaagaag ccgaaatctt ggagccaatt gctgaacgaa
    19081 gcttcgccgc cggcggcact ttgatggaaa ctcgtagcgc agtctcgtcc gctagcatct
    19141 tgaatgtggt tcccggcgct tcccctgcag aacggtcatt cgtattgagt tcacgagacc
    19201 tcgacctttt gctggtggat ttcttgaacg aactcatata cctcttcgat tcagagggct
    19261 tttggggata cagcttcgag ccgaaattcg cggatccgaa atcaaaggac ccggcaggat
    19321 cagtcgatgc cgagatgcgc ttggcagtgc gcacaagcgg tgtgattttg cctccctcgg
    19381 ctcttgaacc tgagcagccg gaggacggca ttcttggaat tgtcccgaaa ggagtatcct
    19441 atcacatgct agaggcggga cagggcaagg atggccgttg gcatgctcag ttcgtgatcg
    19501 atgcgtagtt cggcttactt ggcactatcg aggtaatgtg atctcgagga tttcccagaa
    19561 acttatcgag acttgcttag tgttccggtt ttatcggcat tttcaaggag gtgaacgccg
    19621 tgtcgaagga atggacaggg ccgttggaga ggatagagga atgcatgtgg atgataccaa
    19681 agacttacaa gcccggcatg ctggcggatg cagtagtgat tgcggacgag gaaagtctga
    19741 ggactgcgtg cgaaggcgaa gccttggaac aactggccaa ctccgcattc cttccaggga
    19801 tctgctcgcc agccatggca atgccggata ttcactcagg atacggcttt ccaatcggcg
    19861 gcgttggggc gacggatctc gagtctggcg ttatcacccc cgggggaata ggttttgaca
    19921 tcaattgcgg agtgaggctc gtcaggactg atctggaggc cggggacatt gcccccaaga
    19981 tcaaacctgt aatagacaga ctctaccgcg atatcccgtc gggcctgggc acttcgggca
    20041 agatcagtct cagcgcccaa gaggtgcgca aggttctcca ggaaggagca gagtgggccg
    20101 ttaaagcagg gtatggtttt gaggccgacc ttgagcatac tgaggagggc ggcagcatgg
    20161 agggggctga cgcttccgca gtaagcaaga aggctgtaga gcgtggaatg cctcagctcg
    20221 gaacgatggg atctggcaac cattttctgg aactacaggt tgttgaggag atcgtcaatc
    20281 ctgaggtggc caaggttttc ggcctttttg aggggcaagt ggtgatgatg atacactccg
    20341 gctcgcgggg gttgggcc
//
LOCUS       DAOSAI010000005        19590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_1957_length_19590_cov_3.975122, whole genome shotgun sequence.
ACCESSION   DAOSAI010000005 DAOSAI010000000
VERSION     DAOSAI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19590
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_19590_cov_3.975122"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..627)
                     /locus_tag="PLM83_00390"
     CDS             complement(130..627)
                     /locus_tag="PLM83_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOK69906.1"
                     /translation="MAAFAGESQARNKYTYFAKVAKKEGYEQIGGIFEETAENERAHA
                     KRLIDYVGLVGDTAQNLQHAADGENYEWTSMYPEFERVAREEGFDRIADTFREIGEVE
                     EEHEKRFKALLANVENGTVFSKGQPVKWKCRNCGYVHEGPEAPKACPACAHPQAYFEL
                     FAANY"
     gene            complement(773..931)
                     /locus_tag="PLM83_00395"
     CDS             complement(773..931)
                     /locus_tag="PLM83_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOK69907.1"
                     /translation="MKKWVCTVCGYVYDPAAGDPDNGVAKGTAFEDLPDDWVCPDCGV
                     GKELFEEE"
     gene            complement(1365..1724)
                     /gene="rnpB"
                     /locus_tag="PLM83_00400"
     ncRNA           complement(1365..1724)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLM83_00400"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1766..1948)
                     /locus_tag="PLM83_00405"
     CDS             complement(1766..1948)
                     /locus_tag="PLM83_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOK69908.1"
                     /translation="MKRRCEICGKVIPNERLEALPDTKRCIECARSCGTDVCAKRSEV
                     GMDPDTYKDLLGAVRS"
     gene            complement(2108..2182)
                     /locus_tag="PLM83_00410"
     tRNA            complement(2108..2182)
                     /locus_tag="PLM83_00410"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2148..2150),aa:Ile,seq:cat)
     gene            complement(2185..2259)
                     /locus_tag="PLM83_00415"
     tRNA            complement(2185..2259)
                     /locus_tag="PLM83_00415"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2225..2227),aa:Asn,seq:gtt)
     gene            complement(2923..4032)
                     /gene="rpoD"
                     /locus_tag="PLM83_00420"
     CDS             complement(2923..4032)
                     /gene="rpoD"
                     /locus_tag="PLM83_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOK69909.1"
                     /translation="MTKEFNAPELKNLVAKGKKRGFLTEREIVDALQDVDLTPDQIEE
                     MYEVLGEFGVDIVVGNDSSEADDDVVVDSEDDSADEEEETEIDVSVPEGIALDDPVRM
                     YLKEIGRVPLLTAEEEVELAKRIEQGDEEAKRELAEANLRLVVSIAKRYVGRGMLFLD
                     LIQEGNLGLLKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLVRVQRQLLQELGREPTPEEIAEVMDYPVERVREIMKVAQEPVSLETPIGEEEDSHL
                     GDFIEDEDAIAPPDAASFLLMKEQLEEVLYTLAPREEKVLRLRFGIDDGRARTLEEVG
                     QEFGVTRERIRQIEAKALRKLRHPSRSKKLKDFLE"
     gene            complement(4095..5942)
                     /gene="dnaG"
                     /locus_tag="PLM83_00425"
     CDS             complement(4095..5942)
                     /gene="dnaG"
                     /locus_tag="PLM83_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOK69910.1"
                     /translation="MSSKFSNDLVDKVREANDIVSVVSDYVQLKKVGNRFIGLCPFHS
                     ERTPSFSVSPDRQLFYCFGCHVGGNVFTFIMKMEGLTFPEAVRRLGLRAGIDVSTSVE
                     SPQERAARERRESIYRVNALALAYYRQTLVHSPSAECARDYLKQRRIEADTQEKFKLG
                     YAPASWDSLLTMLAKKGIGPTEVCEAGLALRASGGNRYYDRFRNRLVFPIVNVYGRVV
                     GFGGRALDDSTPKYLNSPESAVFSKRSNLYGIDLASDSMRRSGKAVLVEGYMDVLSAH
                     QAGQTNVVATLGTALTTEQARIVLRYADHVVLCYDADAAGSDATARGVSTTSAVGLDV
                     RVAVLPQGHDPDSFIKEFGGEAFQRSVDEAVPFIRYQLDSLLDAVDWSDIESRLHGLE
                     KVVDVLSGVDSEVERAEYTRELSERFNVDPGALALDVRKARMAAMRGTSRAGPGGNLR
                     SGSRPKPSAWNHGLDDPIWGSKAYLEVERALVRLATESLQNRTRIMETIGPDGFKMPA
                     HQRLFRLLCDVGENGAVTPAELVDCVDAELKDYASSVLFGAKPWESEPDALENALTAW
                     QQFSTRHRLNEIDDEIKEADVAGDYERVYELQAEQKKLRYLLGRDVPLY"
     gene            complement(6228..7250)
                     /locus_tag="PLM83_00430"
     CDS             complement(6228..7250)
                     /locus_tag="PLM83_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOK69911.1"
                     /translation="MTVRERLEEEELERLSPHAAFSRNTRGRDREEEPDEFRTCYQRD
                     RDRIIHCKAFRRLKHKTQVFLSPEGDHYRTRLTHTLEVAQIARTMARALQLNEDLTEA
                     IALGHDLGHTPFGHSGEAALNEVVDGGFRHNEQSLRVVEFVERREGKPGRGLNLTWEV
                     RNGILCHSADWPEEAATLEGQVVKCADKIAYVNHDVDDAMRAGIINEGMIPEEAQRIL
                     GRTRSERLDTLVKDVIDESTNRGKIAMSPEVAQGMAALRNFMFERVYIGSVAKIEADK
                     AKRLLIQLYCYYLDNVDELIEESGESLQGEDVVRIAVDYIAGMTDRYAMKQWTERFVP
                     AAWGVY"
     gene            complement(7389..10040)
                     /gene="ppdK"
                     /locus_tag="PLM83_00435"
     CDS             complement(7389..10040)
                     /gene="ppdK"
                     /locus_tag="PLM83_00435"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOK69912.1"
                     /translation="MGKNNMPKYVYYFGGKQADGKGDMKNLLGGKGAGLAEMTNLGIP
                     VPPGFTITTEVCTAFYENDRKWPEGLEEQIRENLKRLEEEMGAKFADPDNPLLLSVRS
                     GARVSMPGMMDTVLNLGLNDATAQGLIKKTGNPRFVYDSYRRFVHMYSDVVLEVPHEA
                     FEEAIAAKKKARGVKLDMDLTADDLKELVEEYKEIARKHTGKDFPEDPWEQLRGSINA
                     VFDSWNNQRAITYRKINGIPGDWGTAVNVQTMVFGNMGETSGTGVAFTRDPATGENVF
                     YGEYLMNAQGEDVVAGIRTPKPISELKKSMPEIYAQLEQIYHTLEKHYKDMQDIEFTI
                     QDGRLFMLQTRNGKRTGTAAVRIAVEMVEEGLIDKKTAVLRVPPAQLDQLLHPMIDAK
                     AKVDVIAKGLPASPGAAVGRVVFTAKAAEEWAARGEKVVLARTETSPEDIGGMHVAVG
                     ILTSRGGMTSHAAVVARGMGTCCVAGCGALIINEKEKRITCGDLTISEGDWITLNGST
                     GEVILGQVPLVEPELTGNFGKLMEWADEFRALKVRTNADTPEDAKQARYFGAEGIGLC
                     RTEHMFFAEDRIRAMREMILADTEEQRRTALAKLLPYQKGDFMGIFREMDGLPVTIRL
                     LDPPLHEFLPSLEQSKQIEELASEMGIAEQKVRDRIGALHELNPMLGFRGCRLGVVFS
                     EIYEMQTRAIFEAACELVREGVNVMPEVMVPLVGTTGEMEMIREYVVGIADKTIKEYG
                     VDLDYMVGTMIEVPRAALIADEIAKSAEFFSFGTNDMTQMVFGYSRDDAGKFLGEYMD
                     RNVLKHDPFQSLDQEGVGQMVTMGVERGRSTRPDLKIGVCGEHGGDPDSVEFFHKAGL
                     DYVSCSPYRVPIARLAAAQAEIKQPRK"
     gene            complement(10128..12239)
                     /gene="glyS"
                     /locus_tag="PLM83_00440"
     CDS             complement(10128..12239)
                     /gene="glyS"
                     /locus_tag="PLM83_00440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOK69913.1"
                     /translation="MVQSDRVGAVLEIGTEELPARFMPGILEDARTTAASVLEEARLD
                     VGRVRTYATPRRIAIILDDVAPSQADLVREVRGPATRIAYDEDGNPTRAAEGFARSAG
                     ISVAELVRKETPQGEYVYAIVREDGCDAATVLSEAFPRMIDSISFPKSMRWGWGELCF
                     ARPIRWILALLGKEVVDFEYHGMSSGRVTWGHRTLAPGPVEIVSSSEYPARLREIGVM
                     LDPQERASTIMSQAQSLAAECGGTPVVEEGLLEEVTFLVEWPTAFAGRFSSDYLALPE
                     PCVITPMVGHQRYFPVRDADGHLMPVFIGVRNGDDYRLDVVAAGNERVLAARLADARF
                     FYDDDMKVPLAERVPHLKNVVFQEKLGTMYDKAERIEVLGRSLISDSSDADILKRAAF
                     LAKADLVTAMVREFTELQGVMGREYALRQGEDEKTAQAIFEHYLPRYAGDILPSTRVG
                     AALSIADKMDTVAGYFAIGLIPSGSADPYALRRQAAGMLAVHAEWRFSKGLDEIARLA
                     VSLYEEAGVVQCSTEQLVKDVLEFLGTRLKVSLLESGIRHDIADAAISSGVGRPACVR
                     EKAYAVASVVDQDWFVRLATAATRVKNIAGRSEPGKCDPELFDDPAEQGLYDAVCKLE
                     ADLGVQEKEDASGLVERDMYIDAMKRTADICDDIDRYFDAVLVMADDDRVRKNRLAML
                     KWVLDIVSSVIDTTQIVFPGN"
     gene            complement(12226..13095)
                     /locus_tag="PLM83_00445"
     CDS             complement(12226..13095)
                     /locus_tag="PLM83_00445"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOK69914.1"
                     /translation="MNFQDLIFALEQYWARQGATIQQPYVIEVGAGTMSPHTFLRVLG
                     PKPWKVAYVQPSRRPADGRYGENPNRLYQHHQYQVILKPNPDDVVDLYLGSLSAMGIN
                     PKEHDIRLVEDNWEAPTLGAWGTGWEIWLDGMEITQFTYFQQVGGIDTRPVSVELTYG
                     LERISMYLQGVDNVYDMMWADGIAYGDMYHRAEYEFSKYAFEYADIGILSDSFDNYER
                     EAVRCIGAGLLLPGYDYVLKCSHVFNVLDARGAFGAAQRAEYMGRVRALARKVAVAWV
                     RSEEEEAAENGSE"
     gene            complement(13120..13776)
                     /locus_tag="PLM83_00450"
     CDS             complement(13120..13776)
                     /locus_tag="PLM83_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOK69915.1"
                     /translation="MEVDFLRMLAGLYTRALPRAFRSYRRYLLFAAMLLVTCMIVGYT
                     GFSPSGDSLPWIFNSLIGLRDSMAGASTWEQIGMLFWNNLKATLVSIGLGWIFGIVPV
                     ISVCLNGVLIGACTKLMVVRNALSASVVIASFLPHGILELPAFLAGQVVGLRLGFLLP
                     VVWRGRASKNDLGRAAVEGAIILGLFCVPLLAIAAVVELKVTPAVVRMFAPELPTLPL
                     "
     gene            complement(14017..14418)
                     /locus_tag="PLM83_00455"
     CDS             complement(14017..14418)
                     /locus_tag="PLM83_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOK69916.1"
                     /translation="MKVDLDKIDKLRARIDLGYDEAAAYLEAAGGDLVQALIMAERDI
                     GSAVDGSLDLDGGQRESTDGSTWTGRLADVLRRGSEMRLVVERDGANVAYMPVSAGLV
                     GAIFAPELAAVGAILALATRCTMRVEPNPNE"
     gene            complement(14415..15188)
                     /gene="recO"
                     /locus_tag="PLM83_00460"
     CDS             complement(14415..15188)
                     /gene="recO"
                     /locus_tag="PLM83_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOK69917.1"
                     /translation="MALSKCEGVILRVKDFGEADRILTILSDTDGKFDAVARGARRPR
                     SRLVGVTQQFSRVNALLFRGRNLDTLSTVELLSSYSSITSDVMKMAYASYVAELVDLM
                     TGERQRNEMLYALVISTFEALDAGPADPDMVLKAFEIKFMSVSGFRPELARCVSCGKP
                     ENQASWFGPEAGGLLCDGCRSSDWGSVRFSAATRELAKRLLIWDYDMIAVLKPSRSSF
                     EELNNAFRAYVDQRVGRKLHSLDFISDLKSMERGGKQDS"
     gene            complement(15287..15865)
                     /locus_tag="PLM83_00465"
     CDS             complement(15287..15865)
                     /locus_tag="PLM83_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008537195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOK69918.1"
                     /translation="MNLLLHSCCAPCTTYCLEKLRSDGHDVYGYFYNPNIHPYVEFRR
                     RLDTYRDYCASVGLDADICESYGLRQYIVEVGSDIDGRCAVCYRMRLLSAAKLALEKG
                     VDAFTTTLSISPYQNHELLARVGEDVSREVGVPFIYYDFRPGYRRSVEISRELGLYRQ
                     PYCGCIFSEEERYSPKFRKSRKRTKKGSADKE"
     gene            complement(15862..16890)
                     /gene="ruvB"
                     /locus_tag="PLM83_00470"
     CDS             complement(15862..16890)
                     /gene="ruvB"
                     /locus_tag="PLM83_00470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOK69919.1"
                     /translation="MEERIVTGEKTDEDRGSELSLRPRRLSEFIGQNRVKESLSIFIE
                     AARSRGEPLDHVLLYGPPGLGKTTLANIISVEMGVRLRVTSGPAIERAGDLAAILTNL
                     APGDVLFVDEIHRLGRPVEEILYPAMEDYALDIIIGKGPSARSIRIDLPRFTLVGATT
                     RTGLISSPLRDRFGVVHRLELYSVDDLATIVSKSAEILGIEIEALGAYEIARRSRGTP
                     RVANRMLRRVRDYAEVRADGVITQDVACDALAMFDVDELGLDRLDRKILATIIDKFGG
                     GPVGVDTIAAAINEERDTIEDVYEPYLMQLGFIDRTPRGRVATSLAYQHIGREFPTDE
                     ADGQARLL"
     gene            complement(16906..17514)
                     /gene="ruvA"
                     /locus_tag="PLM83_00475"
     CDS             complement(16906..17514)
                     /gene="ruvA"
                     /locus_tag="PLM83_00475"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOK69920.1"
                     /translation="MISQLRGKVASIERDAIVLDVGGIGFSVSVTSGTRATLGRIGEE
                     VRLFTAMVIRDDCVNLYGFESVEEREFFHMLCEVKGIGPKTALSILSSLSPETLSMAI
                     SSGDIAMLATAPGVGKRTAERLAVELKGKVGQFISDEFIERGASANADSTAAQDAISA
                     LVSLGYSPVEAERAVAAAVAAGYSDDASAIIRAALSRMSRAE"
     gene            complement(17511..18017)
                     /gene="ruvC"
                     /locus_tag="PLM83_00480"
     CDS             complement(17511..18017)
                     /gene="ruvC"
                     /locus_tag="PLM83_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOK69921.1"
                     /translation="MLVLGIDPGTAITGYGLVQEANGRIECKDMGCVLTRADLPLAVR
                     LSQIYDELFEIIRRDKPDCAAVEQLFFNTNAKSALAVGHARGVALLACEKAGIPVAEY
                     TPLQVKMAVAGYGRADKKQVQSMVKLLLALDSEPKPDDVADALAIAICHLSTAPYLAK
                     ISGDGDRL"
     gene            complement(18157..18921)
                     /locus_tag="PLM83_00485"
     CDS             complement(18157..18921)
                     /locus_tag="PLM83_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK69922.1"
                     /translation="MSGHSKWANIKHKKAKADAQRGAAFTKASRELHVAVKQGGPDPE
                     ANFRLKMAIQAARAVNMPNDTIQRAIKRAAGDGDGESYEEIVYEGYGPGGVALVVEAM
                     TDNRNRTASDVRHIFSRHGGNLGETGCVNWMFDRKGSITVDTESFAGDEDEMMMIALD
                     AGAEDLKAYDDYYEIITSPEDLDAVRKAMESAGVEYSGARIIRVPQNVMVLGTKEAGP
                     AMRLVDALDEHDDVQHVYTNFDIPDEVLEELEKEGA"
     gene            complement(19038..>19590)
                     /locus_tag="PLM83_00490"
     CDS             complement(19038..>19590)
                     /locus_tag="PLM83_00490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOK69923.1"
                     /translation="TVYDLAEFNEPMDGIVYAVSAGLGFAAFENVLYAASYGLPVGIV
                     RGALTSLVHASFSGIVGFQMGIAKFAPKSMEGPLVARGLMTASVLHGLYDFLLMTELM
                     NFYLTLLVVVLLYVGLAGKIRTALHVSPFRFAELYGKEDGRSEDSVEIGRIGDAAGTG
                     DPDVPSGNGRVANGEDDSSSSRR"
BASE COUNT         4501 a         6136 c         4364 g         4589 t
ORIGIN      
        1 accacactta gggcactttt cgggaggcgt actgccatca tggatatagc cgcacactcc
       61 gcatctccat agcttcatgc ataagccctc ctcgcctcac atgtcgcttc ttgctcgagg
      121 acagtaacat caatagttcg ccgcaaacaa ctcgaaatat gcctgaggat gagcgcacgc
      181 agggcaagcc tttggcgctt ctggaccttc atgaacgtaa ccgcagttcc ggcacttcca
      241 cttcacgggc tggcccttgc tgaacactgt gccattctct acgttggcga gcagggcttt
      301 aaacctcttc tcgtgctctt cctccacctc tccaatctct cggaaggtgt cggcaattct
      361 gtcaaatccc tcttcgcggg ctactcgctc aaactccggg tacatgcttg tccactcata
      421 gttttcgcca tccgcagcat gctgcagatt ctgggcagta tcaccaacaa gtccaacata
      481 atcgatgagc ctcttggcat gagccctctc attctccgcg gtctcctcga agattccccc
      541 gatctgctcg tagccttcct tcttcgctac tttcgcaaaa taagtatact tgttgcgcgc
      601 ctgcgactct ccggcaaacg cagccataat gttgtcgtac gtcttggtac cctccaactt
      661 attagccaag atcatcatcc tcccatatct gatgctagca gaccaacaac cgccgtacta
      721 actgaaacgc ctggtgcacc tctcaaactc caacgcttgc cagttttgcc ttttactctt
      781 cttcaaacaa ctccttgcca acaccgcaat cagggcacac ccaatcatcg ggcaagtctt
      841 caaaggccgt gcctttggct acaccgttat ccgggtctcc ggcagcaggg tcatacacat
      901 agccacagac agtgcacacc catttcttca tatccctgtc ccccttccca tgtcctacat
      961 taacgcagtc tagctctacg ggctcacatt attcgtatac catcatgggg tatgttgtca
     1021 atagtcgttc cttccaggaa cccgacgctc tgcctgacac gtgagggatc ttccgccgaa
     1081 caggcatcca cggtctttcg tttttggcta gggtatccga agagcaagcg ctctatgatt
     1141 aagacattct gcaagttttc ctgatccaat cgcatacaga aaggtgagcc acgtggctgg
     1201 ggatagttga tttcttaaat gaacgctgga aggcgcgtgt cgtttagccg tattgtggcc
     1261 cgttcaacca atacattttg gcatcgcggg catcgatcct gatagcacct gatccagccc
     1321 attcttgaga aatggcgatg cccggcataa gccgggcatc acatgaaaca agtagcccta
     1381 taagccgagt tctgtcatga gtgatcatct atctaggacc catgttgcca tgggcctcaa
     1441 gcgacctaac ccgagggaag agcgggcaac tctattcccc tcctatttgg tcttgctccg
     1501 agtggggttt acctagccgc ccggtcaccc ggacgctggt gcgctcttac cgcaccgttt
     1561 cacccttacc ttgctccgaa caactgtcga tagcagggcg gtctgatttc tgtggcactt
     1621 tccttgaagt caccttcact gggcgttacc cagcactctg ccctatggag ctcggacttt
     1681 cctcaggcgg ccaaaaggcc acccgcgacc actcaggcta cttgcgctct caacatcatg
     1741 atgctaacac tacatccgtt tctcgtcaac ttcgaactgc tccgagcaga tccttgtatg
     1801 tgtcaggatc catgcctact tcgcttctct tggcgcagac gtcggtgcca caactcctcg
     1861 cgcactctat gcaacgcttg gtgtctggga gagcttccag tctttcattg ggtattacct
     1921 tcccgcaaat ctcacatctc cgtttcacaa cacggctcgc ctccaatgca gaatctggag
     1981 aattggcacc ctcttaatta tatttctaca ctccaagcaa agttcctttt atcgatcaca
     2041 tgtaacttac tgtatataca cgaataggca gacaaactgt ccgcctagca ctgcatctgt
     2101 aataatttgg tgggcccacc aggattcgaa cctgggacca atcggttatg agccgaccgc
     2161 tctaccgctg agctatgggc ccaatggctc cccgagcagg attcgaacct gcgacccacc
     2221 ggttaacagc cggttgctct accgctgagc tatcggggaa ctaacttgca aaaagtatta
     2281 taccgtacgc acattcgagg gtcaacacca cataaggcag catttgcatt gcagtcatct
     2341 tctcatcaac gatttccggc atcctaaccg ccatttcatc tatgagggta tacgcctact
     2401 ttatctcgct cctcgatgcc ctcgtaatcc gagaggctac aggcaactcg taaacggcat
     2461 ccgcgacgag atgcagttta tacccaaacc aactcatgat cctgtcccta cgctgccatc
     2521 tttgctcata tgtcttcact ccgaaatccg cgtcgatatt cctgcggcca tcatagctat
     2581 cgaaactccc atcaaccttt tattgatgcc tcatcagttt gcacaagaat ctggtgcatg
     2641 ccgatggcaa atgaacaagg ccggcccagt ttcggggcta tgtgttccgc cactgccatt
     2701 gacatcttgt ccgaaaagaa aaggcggctt cggccccatg caccaccagc atggtcaaca
     2761 cagccgccaa aattcgatct ccattcctcg tctggccaac aagccaagcg ccaatgccta
     2821 aagtgatggc atgccccgcg atcagcttac cccttcaaca tgccagaccc aacggaacta
     2881 atacatccgt acatctccac ctaaggtgaa atatgcacaa acttattcta ggaaatcctt
     2941 gagcttcttg cttcggctcg gatgtcgcag tttgcgcagg gccttggctt ctatctggcg
     3001 tatgcgctcc ctggtcactc cgaactcctg tccaacctct tcaagcgtcc gagcccgacc
     3061 gtcgtcaatt ccaaatctca gtctcagaac cttctcctca cgcggcgcca gagtgtaaag
     3121 cacctcttcc agttgttctt tcatgagaag gaacgaagca gcatcgggcg gagctatggc
     3181 atcctcgtct tcgataaagt caccaagatg cgaatcctct tcttctccga tgggggtttc
     3241 cagggaaaca ggctcctgcg ctaccttcat gatctccctt acccgctcca cggggtaatc
     3301 catgacctcg gcaatctcct ccggcgtagg ctcccggcct agctcctgca aaagttgcct
     3361 ttggacacgc acaagcttgt ttatggtctc caccatgtga acaggaatgc gtatagtcct
     3421 tgcctgatca gcaatggccc tggtaatggc ctgccttatc caccaagtgg cgtaggtgct
     3481 gaacttgtaa ccctttcgat aatcgaactt ctccaccgct ttcaagaggc ccagattgcc
     3541 ctcttgtatc aggtcgagaa agagcatgcc acgaccgaca taccgtttag ctatgctcac
     3601 aaccaacctc agatttgcct cagccaactc gcgttttgcc tcttcgtcgc cttgctctat
     3661 cctcttcgct aactctactt cctcttcggc agtgagcaag ggaactctcc cgatttcctt
     3721 cagatacata cgtaccggat cgtccagggc aatcccctcg ggaacagaca cgtctatttc
     3781 ggtttcctct tcctcatcag ctgaatcgtc ttcagaatca acaactacgt cgtcatcggc
     3841 ctcgctactg tcgttgccca ccacgatgtc taccccgaac tcccctagca cctcgtacat
     3901 ctcctcgatc tgatccgggg tgaggtctac atcttgcaac gcatccacta tctcacgttc
     3961 agtgagaaag cctcgcttct tgcctttagc gacgaggttc ttcaattcgg gcgcgttgaa
     4021 ttcctttgtc atcttttcct caccttccga atcgccttgc gcaaagccga cctgcttgcc
     4081 aacaaccata gcaactaata aagagggaca tcgcgtccaa gcaagtaacg caacttcttc
     4141 tgctctgcct ggagctcata tacgcgctca tagtcccctg caacgtcggc ttccttgatc
     4201 tcgtcgtcaa tttcattaag ccggtgcctt gttgaaaact gctgccatgc tgtgagcgcg
     4261 ttttctagag catcaggttc tgattcccat ggtttcgcgc caaacagcac agatgaggca
     4321 taatccttca attcagcatc cacacaatcc accagctcgg ccggggtcac agccccattc
     4381 tcgcccacat cacatagcag gcgaaacaat ctttgatgtg ctggcatctt aaaaccgtct
     4441 ggtcctattg tttccataat acgcgtccgg ttctgaagag actctgtcgc caaacgaact
     4501 aatgcccttt cgacttcaag atacgctttg ctgccccaga tcgggtcatc aaggccatga
     4561 ttccatgctg acggcttggg cctcgacccg gaacgcaaat tccctccagg gcctgcccgg
     4621 ctcgttccac gcatggctgc cattcgcgcc tttcgaacat ccagagccag agcacctgga
     4681 tccacgttaa acctttctga aagctctctt gtgtattcgg cccgctcgac ttcgctgtca
     4741 actcctgata aaacatccac taccttctca agcccatgca ggcgactttc gatgtcagac
     4801 caatcgactg catccagcaa actgtcaagt tgatacctta tgaacggaac ggcctcatcc
     4861 acagaccttt gaaaggcttc gcctccgaat tcctttataa agctatcagg atcatgcccc
     4921 tggggtaaca cagctacccg cacatcaagc cccacagcgg acgtggtact aacgcctcgg
     4981 gccgtcgcgt ctgaccctgc cgcatcagca tcgtaacaga gaactacatg gtccgcgtat
     5041 ctcaacacaa tcctggcctg ttcagtagtg agagctgtcc caagggtggc caccacattg
     5101 gtttggcctg cctgatgtgc agagagcaca tccatgtatc cttccaccag aacggctttc
     5161 ccgcttcttc tcattgaatc agacgccaga tcgatcccat acaggttcga tctcttgctg
     5221 aagaccgccg attcagggct gttcaggtat ttcggtgtcg aatcatccag ggctcgaccg
     5281 ccaaatccca caacccgacc atacacatta acgatgggaa acaccaaacg gttcctgaat
     5341 ctgtcataat acctgtttcc gcccgaagca cgaagggcca atcctgcctc acatacttcc
     5401 gtcggtccga ttcccttctt ggcaagcatc gtaagaagcg aatcccacga ggccggcgcg
     5461 tagcctagtt tgaatttttc ctgagtatcg gcttctattc gccgttgctt cagataatcc
     5521 cttgcacatt cagcagacgg cgaatgaact agcgtctgcc tatagtatgc caaggcaaga
     5581 gcatttactc tgtatatcga ctctcttcgc tcccgcgctg cacgctcctg cggggactcg
     5641 acgcttgttg atacatctat tccggcacgc aggccgaggc ggcgaactgc ctccgggaac
     5701 gtaagccctt ccattttcat tatgaaagtg aagacgttcc ctcccacatg gcacccgaag
     5761 cagtagaaga gctgacgatc gggcgacacg gaaaacgatg gcgtccgctc agagtggaac
     5821 gggcaaagac caataaacct gttccccacc ttcttgagct gcacgtaatc tgacacaact
     5881 gatacaatgt catttgcctc tcttaccttg tcgaccaagt cattactgaa ctttgaactc
     5941 aaaccaacca gcccctgccg gggtactttt cgccggcgat agatattctc ctgccagatg
     6001 cctgtttagt ggcgtcaaac aaaaccccgc ttccgaatct gctcctggcg gaagcgaggg
     6061 catccgaacc cagtgaccac aagaccactt gcacatccgt aattctacct atgatcagat
     6121 ttcccttcct atgatcttca atctttcccc atggcgggca gacgatctac gccgagtata
     6181 atgtctccta atgcacccag cttatgtagg acgatcattc gactgtctca atagaccccc
     6241 cacgcagcag gaacaaatct ctctgtccat tgcttcattg catatctatc tgtcatgcct
     6301 gcgatgtaat ctacggcgat cctgaccaca tcctcaccct gcaatgattc cccggactcc
     6361 tctatcagtt catctacatt gtcaagataa tagcaataaa gctggattag gagccttttc
     6421 gccttatccg cctcgatctt ggcaaccgat ccgatgtata ccctctcaaa catgaaattc
     6481 cgcaatgcgg ccatgccctg agcaacttca gggctcatcg caatcttgcc tctattcgtg
     6541 ctctcgtcaa tgacgtcctt tacgagcgtg tcaaggcgct cagatctcgt gcgtcccagg
     6601 atccgctgcg cctcctctgg aatcatgcct tcgttgatga ttccagcgcg catagcatca
     6661 tccacatcgt ggttaacata cgcgatcttg tcggcgcact ttacgacctg tccctcaagt
     6721 gtagcagcct cttccggcca atccgccgag tggcaaagta tcccgttgcg gacctcccat
     6781 gttaggttaa gccctcgccc gggcttgcct tcccttcgct caacaaactc taccactctc
     6841 aaactttgct cgttgtggcg aaacccgcca tccacaacct cattgagggc agcctcgcca
     6901 gaatgtccga atggcgtatg ccccaagtca tgcccaagag ctatggcttc agtaaggtct
     6961 tcattgagtt ggagcgcccg tgccatggtc cgagcaatct gggcaacctc gagggtatga
     7021 gttagccgtg ttcgatagtg gtctccttca ggagagagaa aaacctgtgt cttgtgtttc
     7081 agcctccgaa aagccttgca atgtatgatc ctgtccctgt ctcgctggta gcaggtgcga
     7141 aattcatccg gctcctcttc gcgatcacgc ccgcgagtat tgcgcgagaa tgcggcatgc
     7201 ggtgaaagcc tctccagttc ctcttcttcc agacgctctc tgaccgtcat ggcacactcc
     7261 tcctccctat gattattgcc gtaagtggcc cacgcggcat ctccgccacg tgggccacag
     7321 actactctca gatagggagc tccgtctcgt aagttgaagt catcctgttg ggtttcgctc
     7381 cgcgctccct actttcttgg ctgcttgatc tcggcctgtg ctgcagcaag gcgcgcgatc
     7441 ggcacacgat aaggagaaca gctaacgtaa tcaagcccgg ccttgtggaa gaactccacg
     7501 gaatcagggt ctccaccgtg ctcaccacat acgcctattt tcagatcagg cctggtagac
     7561 cgtccgcgct caacacccat ggttaccatc tggcccacgc cttcctggtc cagcgactgg
     7621 aacgggtcat gtttcaaaac gttgcggtcc atgtattcgc cgaggaactt ccctgcgtcg
     7681 tcccggctgt atccgaagac catctgcgtc atgtcattgg taccgaagga gaagaactca
     7741 gcagacttcg ctatctcgtc agcgatcaac gcagcccgcg gaacctcgat catcgttccg
     7801 accatgtagt caaggtcgac tccatattcc ttgatcgtct tgtccgctat gccgacaaca
     7861 tattcgcgta tcatctccat ctcgcctgtg gtcccaacga gaggcaccat tacctcgggc
     7921 attacattta ccccttcgcg aactagctcg cacgcagcct cgaatatggc ccttgtctgc
     7981 atttcataga tctcggagaa cacaacacca agtcggcagc ctctgaaccc caacatcgga
     8041 ttgagctcgt gcagagcgcc gatcctgtct cgcactttct gctcggcgat ccccatctca
     8101 gacgccaatt cctcgatctg cttgctctgc tcgaggcttg gcaggaactc gtgaaggggt
     8161 ggatcgagca acctgatggt tacgggcaga ccatccattt cccggaaaat ccccatgaag
     8221 tcgccttttt ggtacgggag cagcttggca agagctgtgc gcctctgctc ttcagtgtca
     8281 gcaaggatca tctcgcgcat ggcgcgtatc ctatcctcgg cgaagaacat gtgctcagtt
     8341 cggcacagtc cgattccctc agccccaaaa taccgagcct gcttggcatc ttccggcgta
     8401 tccgcattcg ttcggacctt caaagcgcgg aattcatcgg cccactccat gagcttcccg
     8461 aagtttcctg taagctcggg ctccaccaac ggaacctgcc ccaatatgac ttcacctgtg
     8521 gatccgttca gagtgatcca atcgccttct gaaatagtaa gatcgccaca ggttattctc
     8581 ttctccttct cattgatgat gagagcaccg caacctgcca cacaacacgt tcccatgcct
     8641 ctggcaacaa cggctgcatg gctcgtcatt ccgcctcgac tggtaagtat gccaacggca
     8701 acatgcatgc cgccgatatc ttcaggcgat gtctcggtcc tagcaaggac taccttctcc
     8761 ccacgagccg cccattcctc cgcagcctta gcggtgaaga caactcttcc gactgcagcg
     8821 cctggcgatg ctggcaagcc cttggctatc acatcgacct tggccttagc gtcgatcatc
     8881 gggtgcagca gctgatcgag ctgggctggc ggaactcgga gaaccgctgt cttcttgtct
     8941 atcaagcctt cctcgaccat ttctactgca atgcggactg cagctgttcc ggttctcttg
     9001 ccgtttctcg tctggagcat gaagagacga ccatcctgaa tcgtgaactc tatatcctgc
     9061 atatctttgt agtgtttttc aagggtatga tatatctgct ccaactgggc atatatctcc
     9121 ggcatggact ttttcaactc gctgatgggt ttgggagtcc ttatacctgc aacaacatcc
     9181 tcgccctgag cattcatgag gtactcgcca tagaagacat tttcgcctgt tgcagggtca
     9241 cgcgtaaagg cgactcccgt acctgaagtt tctcccatgt ttccaaagac catggtttgg
     9301 acgtttaccg cagttcccca atccccaggg attccgttta tcttgcgata agttattgca
     9361 cgctggttat tccacgaatc gaagactgcg ttgatcgatc ctcgcagctg ttcccaagga
     9421 tcttcaggga aatccttgcc tgtgtgtttg cgcgctattt ccttgtactc ttccaccagt
     9481 tccttcaggt cgtccgcagt gagatccatg tcgagcttga ctccgcgagc ctttttctta
     9541 gcagctattg cctcctcaaa ggcttcgtgg ggaacctcca agacgacatc ggaatacatg
     9601 tgcacaaacc gtcgatagct gtcgtagacg aaccgaggat ttcctgtttt cttgatgagc
     9661 ccttgggcag tagcatcgtt caaaccgaga ttgaggactg tgtccatcat gcctggcatt
     9721 gaaacacggg caccagacct tactgaaagc aaaagcggat tatccggatc ggcgaacttg
     9781 gcacccatct cctcttcgag gcgcttgaga ttctcccgaa tctgttcctc tagcccttcg
     9841 ggccatttcc gatcgttctc ataaaaagca gtgcagacct ccgtggtgat agtaaaaccg
     9901 ggcgggacag gaatccccaa gttggtcatt tcagcaagac ccgctccctt gccgcccagg
     9961 aggttcttca tatcgccttt accgtcggcc tgtttacctc cgaagtagta gacatacttt
    10021 ggcatgttat tctttcccat gctaatcccc ctatctaatg cagcaatcta cgcatcaaat
    10081 ggcattgaat tctactcaaa ctctcttttt ccttcttctg cgcccggcta atttccgggg
    10141 aagactatct gtgtcgtgtc aatgaccgaa gatacgatat ctaaaaccca tttgagcatt
    10201 gcaaggcggt tcttcctgac tcggtcatca tccgccatga ccaacaccgc atcgaaatat
    10261 cgatcgatgt cgtcacagat atcagccgtt ctcttcatgg catctatata catatccctt
    10321 tccaccaaac cagacgcatc ttccttctcc tgcacgccca aatccgcctc gagcttgcaa
    10381 acagcgtcat acaggccttg ttcagcagga tcatcaaaga gttccgggtc gcacttgcca
    10441 ggctcagaac gcccagctat gtttttgact cgcgtcgcag cagtagccaa acgcacaaac
    10501 cagtcctgat cgactacaga cgccaccgca tacgccttct cacgaacgca agccggccgc
    10561 cccacacccg aagaaatggc agcgtctgca atgtcatggc gtatccccga ttccagtaac
    10621 gataccttga gcctcgtgcc taggaattcg agaacatcct ttacaagctg ctcagtggag
    10681 cactgcacaa ccccggcctc ctcgtacagg ctgaccgcaa gccgcgcaat ctcatccagg
    10741 cctttggaaa acctccactc agcatggacc gcgagcatgc ccgcggcttg ccttctgagc
    10801 gcgtacggat ctgccgatcc ggacgggatg agcccgatcg caaagtagcc cgcaacggta
    10861 tccatcttgt cggctatgct cagcgcagcg cccacacggg tgctgggcag aatgtcccct
    10921 gcgtatctcg gcaagtagtg ctcgaatatt gcctgagcgg ttttctcgtc ctcaccttgc
    10981 cgtagagcgt attccctgcc cattacgcct tgaagctccg taaactcacg aaccatcgct
    11041 gtaaccaggt cagccttggc caagaacgcc gcccgcttga gaatatctgc atcactagaa
    11101 tcagatatca agctgcgtcc gagaacctcg atgcgttccg ccttatcata cattgtaccc
    11161 agtttctcct gaaagaccac gttcttcagg tgcggcaccc tctccgcaag cggcaccttc
    11221 atgtcgtcgt cgtagaagaa acgcgcatct gccagcctcg cagcgagcac ccgctcgttc
    11281 ccggccgcca ccacgtcaag acggtagtca tcgccattac gcactccaat aaacacaggc
    11341 atcagatgcc catcggcgtc ccgcaccggg aaataacgct gatgtcccac catcggtgtt
    11401 ataacgcatg gctcaggaag cgcaaggtaa tcgctggaga accttcctgc gaaggccgtt
    11461 ggccactcaa ctagaaacgt gacctcttcc agaaggccct cttccacgac aggggttccc
    11521 ccacattccg ccgccagcga ctgcgcctgg ctcatgattg ttgaagcccg ctcctgggga
    11581 tctagcatta ctccgatctc ccgaagccgc gccggatact ccgagctcga aacgatctcc
    11641 acagggcctg gcgccagcgt ccggtgtccc catgtaactc gtccactgga cataccgtga
    11701 tattcgaaat ccacaacttc cttgcctaac aaagccaata tccatctgat gggccgcgca
    11761 aaacacagtt ccccccaacc ccatctcatc gatttcggaa agctgatcga atcgatcatc
    11821 ctggggaacg cttcactgag cactgtcgcc gcgtcacatc catcttcgcg gactatggcg
    11881 tatacatatt caccttgcgg agtctctttc cgcaccaatt cagccacaga gattccggca
    11941 gagcgtgcga atccttcagc agcgcgagtg ggattcccgt cttcatcgta agcaatgcgg
    12001 gtcgcaggac cgcgaacctc acgcacgagg tcggcctggg atggcgctac atcgtcaagg
    12061 attattgcaa tcctacgcgg ggtggcataa gtgcgaaccc gaccaacatc aagacgggcc
    12121 tcttcaagaa ccgaagcagc tgttgttctg gcgtcttcca ggattcctgg cataaacctc
    12181 gcgggcagct cctcagtgcc tatctcgaga actgccccga cgcgatcact ctgaaccatt
    12241 ctctgccgcc tcctcttcct cagaccttac ccatgccact gcaaccttcc ttgccaaagc
    12301 tcggacacgt cccatatact ccgcgcgttg ggcagcccca aaagcgcccc ttgcatcaag
    12361 cacgttgaag acatgcgaac atttcagcac ataatcatac ccgggaagca acaggcccgc
    12421 cccgatacat cgtacagcct cgcgttcgta gttgtcgaag gaatcgctga ggattccgat
    12481 atccgcatac tcgaaagcat acttggaaaa ctcatattca gctctatgat acatatcgcc
    12541 ataagcaata ccatcagccc acatcatatc gtaaacgtta tccacaccct ggagatacat
    12601 ggaaatcctt tcgaggccgt atgtgagttc cactgaaaca ggcctcgtat cgatcccacc
    12661 tacctgttga aagtaagtga actgcgttat ctccatcccg tcaagccata tctcccaccc
    12721 ggtgccccag gcacccaagg taggagcctc ccagttgtct tccaccagtc gaatatcatg
    12781 ttctttggga ttgatgccca tcgccgacag gctccccaga tacagatcga ccacatcatc
    12841 gggattcggc ttcaggatca cctggtattg atggtgctga taaagtctgt tggggttctc
    12901 tccgtaccgg ccatcagcag gcctgcgcga cggttgtaca tacgcaacct tccatggctt
    12961 tggccccaaa accctcagaa aggtgtgggg gctcatagtg cctgcgccca cctctatcac
    13021 gtaaggttgt tgaatcgtgg cgccctggcg cgcccagtat tgttccaaag caaatatcaa
    13081 atcctgaaag ttcaagcgcg gtccctcccc atctcatcgt catagaggca gcgttggaag
    13141 ctccggagca aacatccgaa caactgccgg cgtaaccttg agctccacaa cagcagctat
    13201 cgccagcagc ggcacacaaa acaagccaag gatgatcgct ccctcaacag ccgcgcgccc
    13261 cagatcgttc ttgcttgcgc gcccacgcca caccaccgga agaagaaagc ccaaacgcaa
    13321 ccccacaacc tggcctgcca agaaagccgg caactccagt attccatggg gaagaaaaga
    13381 cgcaatcaca acagatgccg atagggcatt tctgacaacc atcagcttcg tacacgcccc
    13441 gatgagcact ccgttaaggc atacacttat tacggggacg atgccgaata tccaccccag
    13501 accgatggag acaagcgtgg cttttaggtt gttccaaaac agcattccaa tctgttccca
    13561 tgtagaagcg ccggccatgg aatccctcag ccctatgagg ctgttgaata tccacggcag
    13621 agaatcgcct gaagggctga atccggtgta acccacaatc atgcaggtga caaggagcat
    13681 agcggcaaag agcaagtatc gccggtaact gcgaaacgcc cggggcagtg cccgagtgta
    13741 aaggccggca agcatcctca aaaagtccac ctccatgaaa aaaagcctcc gcccccgaaa
    13801 gctcgtgggg ggcacgggag gctatgttcc cgaagcgccg ggcgcgttcg cttgcacgct
    13861 gtcatttgcc ctaactatag caaatggccc tgcaatatgt caattcccgg ctctggtttt
    13921 agcaccttcc ctcgtacgcc gaaaaaagct tgcattagat agtcctcccg ctaacgtgga
    13981 tgtaccccga acccggaagg atgatgctcg atccgctcac tcgttcggat tcggctctac
    14041 cctcatcgtg catctggtcg ccagggccaa gatggccccc acggctgcca gctcgggagc
    14101 gaaaatagcc cctaccaatc ctgccgacac cggcatataa gctacattgg ccccgtcgcg
    14161 ttccaccacc agcctcatct cagaaccacg tctgagaacg tccgccaggc gtccggtcca
    14221 tgtcgaaccg tccgtggact cacgttggcc accgtccaaa tccaggctgc catccacagc
    14281 gcttccaatg tctctttccg ccattatgag agcctgaaca agatcgcctc ctgctgcttc
    14341 caaatacgca gcggcctcat catagccgag atcaatccgc gcgcggagct tgtcaatttt
    14401 atcgaggtca accttcaact atcctgtttg ccccctcgct ccatcgattt aagatcagag
    14461 atgaagtcaa gcgaatgcag cttgcgccct acccgctgat ccacataggc cctgaaggca
    14521 ttgtttagtt cttcaaagct gctacgactc ggcttgagca ccgctatcat gtcgtaatcc
    14581 catatcagaa ggcgcttggc cagttcccgc gttgcagcgg aaaatcggac gcttccccag
    14641 tccgaactgc ggcacccatc gcacagcaac cccccggcct ccgggccaaa ccatgacgct
    14701 tggttttcag gctttccaca ggaaacacat cttgcaagct caggtcggaa gccagaaaca
    14761 gacatgaact ttatttcaaa cgccttaagc accatatcag ggtctgcggg ccccgcatcg
    14821 agcgcttcga atgtcgaaat aaccaaggcg tatagcattt cgttacgctg cctttcgcct
    14881 gtcatgagat cgaccagttc agcaacgtat gaagcatatg ccatcttcat tacatccgat
    14941 gtgatgctgg aataagaaga cagcagctcc acagtgctca gcgtatccaa gtttctgcca
    15001 cggaacaata gcgcattcac gcgcgaaaat tgttgagtaa ccccaaccag tctgctccga
    15061 gggcgccgag cgccgcgggc gacagcatca aacttcccat ccgtatcaga aaggatcgtt
    15121 aagatccggt ctgcttcacc gaaatcttta acccgaagaa taaccccttc acattttgac
    15181 aacgccaatc cgcatcatcc tcaacatgcg gaccaccggg gctttgtaat gtaacgttcc
    15241 catcggcgct ccgcgctatg caaggaatac gactgctccc gaaggtttac tccttatcag
    15301 cagagccttt tttcgtccgc ttacgcgact tgcggaactt tggggagtag cgctcttcct
    15361 cactgaatat gcagccacaa tacggctgtc gataaaggcc caattctcgc gagatttcaa
    15421 cgcttcgcct atatccaggc ctgaaatcat aatagataaa gggcactccg acttccctcg
    15481 acacgtcctc acccacccgc gctaaaagct cgtggttttg ataggggctg atagaaagag
    15541 ttgtagtaaa tgcgtcaaca cccttctcca gtgcaagctt tgccgcggaa aggaggcgca
    15601 tgcgatagca gacggcgcat ctgccgtcga tatcactccc aacctccaca atgtactgcc
    15661 gaaggccata actctcacaa atatccgcgt ccagaccaac tgatgcacag taatccctgt
    15721 atgtgtccag ccttcgccga aactcgacat acggatgtat gttggggtta tagaaatacc
    15781 cgtatacatc atgcccatca gacctcagct tctcgaggca ataggtagtg cacggggcac
    15841 agcaagaatg gagcagcaaa ttcacagcaa cctcgcctgc ccatctgctt catctgttgg
    15901 aaattcccgt cctatgtgct ggtatgccaa gcttgtagcc actcgaccgc ggggcgtcct
    15961 gtctatgaaa cctagctgca tcaagtacgg ctcatacaca tcctcgatag tatctctctc
    16021 ctcattgatg gcagccgcga tagtgtccac tccgaccggt ccacccccga acttgtctat
    16081 tatagttgct agaatcttcc tgtcaagcct gtcgaggcca agttcatcca catcaaacat
    16141 ggccaaggca tcacaagcca catcctgtgt tatgacgccg tctgcgcgca cctcggcgta
    16201 atcgcgtacc cgcctcagca tcctattggc caccctaggg gttcccctgg accgcctcgc
    16261 tatctcgtaa gcccctaagg cctcgatctc aattcccaag atctcagcgg attttgagac
    16321 gattgtggca agatcgtcta cagagtatag ctctagcctg tggactactc caaacctatc
    16381 gcgaaggggc gaggatatca gtcctgtcct agttgtggcc cccaccagag taaatcttgg
    16441 caggtcgatc cgtatcgatc ttgcagacgg gcctttgccg atgatgatat cgagcgcata
    16501 atcctccatg gccgggtaga gtatctcctc aaccggcctt cccagcctgt ggatctcatc
    16561 cacgaacagc acgtccccgg gggcaagatt ggtaagtatg gcagccaagt cccccgcgcg
    16621 ttctatagcc ggcccagacg tgacccgaag ccttacaccc atctccactg agatgatgtt
    16681 ggccaacgtc gtttttccga ggcctggggg accatacagc agcacgtgat cgagaggctc
    16741 gcccctactt ctggcggctt ctatgaaaat cgagaggctc tcctttactc tgttttggcc
    16801 aatgaactca gagagccgcc ttggacggag gctcaactca gacccgcgat cttcatctgt
    16861 cttttccccc gtcactattc tttcctccat agaaatgccc tccgatcatt ccgcgcgcga
    16921 cattcttgag agcgccgcgc gaataatggc agaagcatcg tccgaatagc cggctgccac
    16981 ggctgcagcc accgcccttt cggcttcaac aggtgagtag cctagcgata caagcgcact
    17041 gatcgcatcc tgggcagcag ttgaatctgc gtttgccgat gcgcctctct ctatgaattc
    17101 gtcggagatg aactgcccaa ccttaccttt cagttcaact gcaagtcgct ccgccgtcct
    17161 ctttcccacc ccgggagctg tagccaacat cgcgatgtca cccgaagaga tagccatgga
    17221 aagcgtctcc ggagaaagcg aggataggat cgacagcgca gttttagggc caatcccctt
    17281 gacctcgcag agcatgtgga agaattctcg ctcctccaca ctctcaaaac cataaaggtt
    17341 cacacagtca tcgcgaatca ccattgcggt aaaaaggcga acttcctccc ctattcgccc
    17401 caaagtagct ctcgttcccg acgtcaccga gacagagaac ccaatgcccc caacatccaa
    17461 aacaatggcg tcccgctcga tcgaagccac cttgccgcga agctgagata tcatagacga
    17521 tcaccatccc cggagatctt tgcaagatac ggagcggtag atagatggca tatggctatc
    17581 gccagcgcat ctgcaacatc atccggcttt ggttccgaat ccaacgccag caaaagcttg
    17641 accatgctct gcacctgctt cttgtctgcc ctgccatacc cagcaactgc cattttcacc
    17701 tgaagaggag tatactccgc caccggaatg cccgctttct cacacgctaa aagcgcgacg
    17761 cctcgcgcgt gtcctaccgc caaggcgctc ttggcgttag tattaaagaa aagttgctca
    17821 accgcagcgc aatccggctt atcacgcctg ataatctcaa aaagttcatc ataaatctga
    17881 gaaagcctga cagcaagagg gaggtctgcc cgagtaagca cgcaccccat atctttgcat
    17941 tcaattctgc catttgcttc ctgcaccagg ccatagcctg taatagccgt cccgggatcg
    18001 atgccaagca cgagcaccat cgccacctcc cattcatagc aactaataac cgtgagctaa
    18061 ggcaagcccg tccgcggcat attgcaaaca tgcacgccac atagaaaagc ggccacgcct
    18121 cgtgcgtggt cgccatggga aggaagaagc acttgctcaa gctccttcct tctctagctc
    18181 ctcaagcact tcgtcgggaa tgtcgaaatt cgtgtacacg tgctgcacat catcgtgctc
    18241 atcgagggcg tcaacgagac gcattgccgg gccagcctct ttggttccaa gaaccataac
    18301 attttgcgga actcgaatta tccgagcacc agagtattcg actccagcac tctccatggc
    18361 cttccgcacc gcgtcaagat cctcgggcga ggtgatgatt tcatagtaat cgtcgtaagc
    18421 cttgagatcc tcagctcccg catcaagcgc gatcatcatc atctcgtctt cgtcaccagc
    18481 aaaactttcc gtatccacag ttatcgagcc tttcctgtcg aacatccagt tcacgcatcc
    18541 tgtttcccca agattgccgc cgtgacgcga aaagatgtga cgaacatcgg atgcggtgcg
    18601 attcctgttg tcagtcatag cctctaccac gagagcaact ccacccggcc catatccttc
    18661 atacacgatc tcctcatagc tctcgccatc tccatcgccc gccgccctct taatggcccg
    18721 ctgaattgta tcattgggca tgttaaccgc tcgcgcagcc tgaatggcca tcttgagcct
    18781 gaagtttgcc tctggatcag gcccaccttg tttaacggcg acatgcagtt cgcgcgatgc
    18841 cttagtgaaa gcagcacccc gctgagcgtc agccttagcc tttttatgct taatgttagc
    18901 ccatttcgaa tgtcctgaca tcatgcgtac ccccttccac aggaactcca atgactttgc
    18961 cgcgtacatt ctaacacagc cctagctcgt tggaaaggat tcggcatcag agcataggaa
    19021 gaagagacat gccgccctca tctacgactg ctcgagctgt cgtcttctcc gtttgccact
    19081 cttccattac cagacggcac atccggatca ccggtcccag cagcgtcgcc aatccgtcca
    19141 atctccacag aatcctcaga tcttccatcc tctttcccat acagttctgc gaacctgaac
    19201 ggtgaaacgt ggagagctgt ccttatcttc ccggcgagcc cgacatatag caagacaacc
    19261 acgagcaaag taaggtagaa attcatcagc tcggtcatga gaagaaaatc atacaaacca
    19321 tgaagcaccg acgcggtcat caatcctctg gctacgagcg gtccttccat ggacttcgga
    19381 gcgaacttgg caatacccat ttgaaagccg acgatcccgg aaaaggatgc gtgcaccaag
    19441 gatgtaagcg cacctctgac tatcccaact ggaaggccat aggatgccgc gtagagaaca
    19501 ttctcaaagg cggcaaatcc gagcccggcc gagaccgcgt agactatgcc atccattggt
    19561 tcgttgaact cggcaagatc gtacactgtt
//
LOCUS       DAOSAI010000006        18404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_2108_length_18404_cov_4.187694, whole genome shotgun sequence.
ACCESSION   DAOSAI010000006 DAOSAI010000000
VERSION     DAOSAI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 18404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18404
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2108_length_18404_cov_4.187694"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1492
                     /locus_tag="PLM83_00495"
     CDS             92..1492
                     /locus_tag="PLM83_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOK69924.1"
                     /translation="MLKRINALFGAQDMTEGKPMSVLLRFSIPLLIGNFAQQMYSTVD
                     SIIVGRYVGDKALSSIGTTLPVINLLLVLFMAVSTGAGIMVAQYYGAKDKESLSRSIG
                     NAMTLVFISSLVIMAVGIPLAGQLLTLTKTPVETFDMAYSYLTITLSGILGVGYYNIV
                     SGILRGLGNSVFPLLTLLLASILNIFMDIWFVAGLGMGVAGAAWATIISQAVSAVLCI
                     AKLRRMKDVVNLNASNLVPDKKLTGQLLRLGLPAGITQAVFSMSMVFVQALANSMGYQ
                     VVTCNTAVMRIDGFAMMPNFTFGMAIATFVGQNIGADKMDRVAQGTKDILKVSLTTSF
                     VLVACLLLFGENLIRMFTTTEEIINLGVRQIRILAAGYVAMAISQVFGGIMRGAGDTM
                     PSMWISMFTVVAVRVPLAYILASLTRSPDHPAGSPDALFFSLLISWIAGAVCNYLWYR
                     RGTWREKSLVAHEQAA"
     gene            1818..2732
                     /locus_tag="PLM83_00500"
     CDS             1818..2732
                     /locus_tag="PLM83_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOK69925.1"
                     /translation="MGHRRRSVLFAVFLAVLMIAFATTAHGNSAVGEAGPGNPTEIGV
                     DEKEMVSDSKAEATAKRPLVIAHRGASGVAPENTMAAFERAVEMGADMIELDVHATKD
                     GQIVVIHDAVLDRTTTGSVRGSVVWRTLEEIKTLDAGSWKGAEFAGEKVPTLHEVLST
                     FKGRTKVLIEIKAKGIEDKVVEIIRETKTEDDVIIQSFDVEAVRRCRNLLPQVPAGVL
                     FSAPLLFGKSSFGEKVISTALDVNADFVAVDQGGVTPELVGMARENGLGVFVWTVNKT
                     KDMTKMIQLGVDGIITNHPDVLIGLLAE"
     gene            2984..3853
                     /locus_tag="PLM83_00505"
     CDS             2984..3853
                     /locus_tag="PLM83_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOK69926.1"
                     /translation="MSFGIVTDSSANLTDEMIDKYGIDIISLSYRIGEEECQSYVKGE
                     KTDLKQFYEKLRQGEVAETSLINMRDCENAFESVLQEGKDLLYIGFSSALSGTYNAAS
                     IVADNMREIYPQRKIYAIDSLAASMGQGLLVYHAVEQRLAGRSIDEVKDWLLENRLRL
                     CHWFTVDDLMYLKRGGRVSVSAAVIGTVLSVKPVMHVDDEGKLVPVRNVRGRKKSLVA
                     LVDEMEKTCIDPTEQMVFISHGDSLEDAECVEKLVRERLNVRDVRINYVDPVIGAHSG
                     PGTIALFFLGTKR"
     gene            complement(3959..4663)
                     /locus_tag="PLM83_00510"
     CDS             complement(3959..4663)
                     /locus_tag="PLM83_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOK69927.1"
                     /translation="MRRCTFVAMIVLIGLFASPYALSAEEQQRLMPATIIRVLDGDTV
                     EVILENGARQRVRLIGVDCPELTDKDPSVRDYAFAAAAFTRELLEGRRVWLETDMGTL
                     DKYGRLLAYVWTAPPADFAEEKIRDCMFNAILLLEGYAYTYMLMPNTKYAAVFVGFQH
                     EATMEGRGIWGTMRRWIHIIVYVTNTGKKYHGDGCRYLASSRIPMTLAEAMWRRYERC
                     QTCKPPYWMSAGISLE"
     gene            complement(5433..8045)
                     /locus_tag="PLM83_00515"
     CDS             complement(5433..8045)
                     /locus_tag="PLM83_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK69928.1"
                     /translation="MSVPGVPKPEVAVRPVLDSKQRAFVDCQHGCVVLHAPVGTGKTL
                     ALAERAAEAIRHGVDPNRILCVTFTNRAAEELRQRITVSCGEHARGVVVRTFHSLCAW
                     MLRSEAKQMSLPSGFVIFDDDDSMEIIRQCAKRTGVHLVCSGYDDEARDAQDAITTAK
                     INAPDAVLSTNSVQDAVFDSLPRAWRRLALAYQDELAAHHALDFADLVYFARAMLSVE
                     PDIANRWAERFSMIQVDEMQDTHVSEYQVLRVLAKKYGNIALAGDFDQTIYEWRGSQP
                     EKIITLLQKDFPQARRFSSVTNYRTTRTLVDAAASVAANYSDFDRVRPCATAEEGNPI
                     VVHFARDSQAEAQWIAEETRKLIEPGESANNIDPLRPGRIAVLTRTNWRGTVISEAFA
                     EAGIPHLTVEQFEFFRRQEVKDAIAYLRFLRNPFDGRSFQRMLLRPPRNIGRQTIDSV
                     IEAERTGLRLIDMVNPSTIELGDPFARLMRELDSGSVVVFDTETTGLNPASDDIVELA
                     AIRLEHGQPVATFHRYLRNRVSVGDSRHIHGLSDQFLAAHGADPLETLSQFMAFADGA
                     LLAGHNVSFDVRMFRANARRLGLNPPSHQCADTLDIARRFLDVDDYSLANLTRYFNIP
                     CKPTHRAMDDVNATTALLIALAPYVNKGSIGRMQAVRETGESFVPLAREIEEMLMLAE
                     RVRPDALLDEVLERSGLNAYYIKEPRRMENLRELVRIFRDKDDVRLDPISSLESILHF
                     VALARNVDRVDPEDERVRILTVHQSKGLEFDVVFVAGLSEHEFPGYRSVKDGRQDEEL
                     RLFYVALTRARSRLFLTGHAVNNGKHREPSPYFGTISDGWVEQDSHAIRRTYGSGNRR
                     NPQSLQSKGHRRPF"
     gene            8252..8974
                     /locus_tag="PLM83_00520"
     CDS             8252..8974
                     /locus_tag="PLM83_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOK69929.1"
                     /translation="MRLQGEGVVQKIEHKSVSQQVEEAIKRYIVQKGLRTGDRLPTER
                     ELAEMLGVSRSSVREAIVGLQALGIVESKPKVGITVSEPNVASLIHAVAFNSGLNTEK
                     IFQLNEARLVLETAALKLAVEKADQSDYEAMAETIYRMEAKVEAGESCAAEDLAFHQA
                     IVEATHNPILSQSSHTLQEFFHIISAEGVGAGVSPARILSEHKEIYNAFRQKNVGKCE
                     ELMTRHLQRYYFAQRQEGDTEK"
     gene            8971..9873
                     /locus_tag="PLM83_00525"
     CDS             8971..9873
                     /locus_tag="PLM83_00525"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOK69930.1"
                     /translation="MTADFELKGIIPPLVTPLDDDGALDEQAFRRSIRRMVRAGASGI
                     FILGSSGEFAALTDACKARVIETAVDEVGGKMPVLVGVGDTSTARVFENMQAAALPGV
                     SAIVVCTPYYFPTQQEALVKFYEDIAEKSQVPVVLYNVPQTTKTWIGIETVRRLTDNP
                     NIIGIKDSSGDFDYHQRLISLASSASRFSVLQGDELSIGASVLMGGHGAVPGIANLDP
                     KACVDLYDAAASGDAATAVKMQSGVSSLCEVYKHGGSPVGALKACLKMMGEGSGKLTH
                     PLDDQKPLDDLEILSRILSERGLI"
     gene            9901..10893
                     /locus_tag="PLM83_00530"
     CDS             9901..10893
                     /locus_tag="PLM83_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK69931.1"
                     /translation="MSHDRPRIGIIGLGLSNPYTFARILKAEGADVAYVWDYHRDAAE
                     AFARENGCEAAPTWQELLKAPLDGVLVESINADHCKLAKAFVEQGVPAYVEKPLSFDY
                     EEAKDLLDTAVSNDCPILSCSPLRFSPVFQEIARSVREGRLGLPTLAHARVIHTMHHF
                     ATDPRKRWHDSLSLGGGMVVDIGVHAVELGYMMLGPGAASVYCETSRVKYKDIESEDT
                     AAITIKFADGAIAVCDVVCATNELEYSVMVNGSEDSIDSRELCAKLCNEGYGTPDGIG
                     NALSAYGGFVETMRQFLKCIDRRCFPVPPLEMGEIVKILSAARASAKQHSPVLI"
     gene            10928..12010
                     /locus_tag="PLM83_00535"
     CDS             10928..12010
                     /locus_tag="PLM83_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK69932.1"
                     /translation="MRYRAGIVGCGRIGWKLEDDPNRPKPCTHAGAYASIPNTQIVAA
                     SSRTDKSLKSFGERFGVDALYTDFNEMLEKENLDLLSVTTNPELHRDIVVKAAECGVR
                     GILCEKPISLSLDDADDMIEACRKSGTVLMVMHNRRWNSYYVKAKELLDLGAIGELVS
                     IFGNCQGCKPSPDWLSDREGPLLHDATHLYDIMRFFAGDIDSVVGSAEKRKHLHYGVE
                     DTCFSHMRFKNGVSAVALVNERTSYMRFDIELQGSDGKILLGPDAEMWQVVPSKRAAG
                     FRELEKVEFPKPEREANVYMDAIGELIHCVETGEPSRSTGEDGRAALEAIMAIYESQR
                     RGNSPVQLPLGVRESLLLALRRENRL"
     gene            12043..12912
                     /locus_tag="PLM83_00540"
     CDS             12043..12912
                     /locus_tag="PLM83_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK69933.1"
                     /translation="MNTLSELRKSRESYYFLAPSVIIFLVFGLSVLVANVVLSFYSWS
                     ALTEPRFIGLDNYRRLFHDAVFRKALVNTLYYCALYVVPSLIISVLLAFVLNKKLKGM
                     NLFRSIFYLPVITSYVIVVILWQWIYDADIGLLNSILNKIGIASVPWLLSPKLALPSL
                     VLMSIWKNVGYTIVIFLAGFQQIDVALHEAARVEGATEWQVFRHITLPLLRPTTLFST
                     VMLIIWSFQMFVQPYMMTHGGPARSTTTLVYYLYETGFQYFDLGYASTMSVVMLIMVL
                     PIIILQSKLLRAR"
     gene            12926..13771
                     /locus_tag="PLM83_00545"
     CDS             12926..13771
                     /locus_tag="PLM83_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK69934.1"
                     /translation="MNRLRKMPMKNFWTYFILVLLGSTMIVPFIAMVSQSLKPGSQVL
                     EYPFRWIPENPSMENYRMLFKQNPIMRWTLNSFIVAGGSTILQLITCSLAAYAFSRTS
                     FPGKDILFWMMMSMLIIPSQTTLVPLFLLFSKLNLTNTYTALLLPTASSAFGIFLLKQ
                     FMEPIPKDYDEAASLDGCTRFQFYSKILLPMCKPALAVLATFNFVTQWNDFLYPLIMT
                     QTRDMFTLQIGLSALQASGSRGEMGGMGVVMAGAVWGFIPTFILFLLLQKYLVEGVSS
                     AGIKG"
     gene            13807..15078
                     /locus_tag="PLM83_00550"
     CDS             13807..15078
                     /locus_tag="PLM83_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK69935.1"
                     /translation="MKSHGKLVRMLRATLLTICVLLLFAASTGLAARQETVTMWAFPL
                     CSDDIEMYSKLVNEFEKENPNIKVHVEVLPWQGRMEKLMAALAANQAPDVMYLNVFLV
                     PKLAATGNLAPLDKYLPSDVRADYFSGAIKSCQYDGRLYAMPVLQSVVTYIYNVDLFK
                     KAGLDPKAPPETWAELEEAARKLTCDTDGDGKIDQWGIRFDLNRPSPITSVVPFVWQA
                     GGDLISADGTEAIYDSPEAVRAVEFIKGLIDKGYMQKSNIQGGGLPFTSGKIGIDLQR
                     ENMDVIKLRAEGLDFEIAAGPILKDAKKIGYGTVGSYGILGKSKNKDAAAKWVLFLTR
                     PDNMAYIMKNTGFVAPRKSVSPAKYVTDPVIKQFVEQAPNCRPETPHLYIQEVLSTMQ
                     PAIENALLGLKPVEAALKEGTAEANRIMRRK"
     gene            15700..16620
                     /locus_tag="PLM83_00555"
     CDS             15700..16620
                     /locus_tag="PLM83_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOK69936.1"
                     /translation="MLLCTHRKGLVLSIIALLAFGYGLFGASCCAGAGVPVASDGGLV
                     GSKRITVMTYNIHYGVGFDFVYDLDRIADLIEEEGADIIGLCEVEQCTKKFNYQDQAG
                     HIADKLGFYYAYGPIFARKDGGYFSNALISRYPIVSHQTHQLPIFNDNQKRAVLEAQV
                     DVDGTLLNVFVTHLEVKDHESRMAQAQALLEIASRTECPRIIMGDFNTIPTSSVIALM
                     LHEYNDTYQVYRVLTDSAEIASEGLFERDYLKGGYTIDPFDPNRRIDFIFSSFDIRVV
                     GEPKAARVHHSMASDHLPYIATLELPAASL"
     gene            16798..17748
                     /locus_tag="PLM83_00560"
     CDS             16798..17748
                     /locus_tag="PLM83_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK69937.1"
                     /translation="MRTVVTGATGLLGEEVADWLAGQEGHEVIGLCGRRDLDVVDTRA
                     VVSFMCRAQPDLIVHCAAERDMDAAERDPKAAYMLNTFGTWNLALAARLCDCKMVFIS
                     SEAVYSGEKDAPYTESDLAAPLSVYGWSKLAAEEEVKSLLERYFILRVPLLFGAKGHS
                     DNNRILNFVRALRSGKCVTAPIDQWTSPTYARDVAMAIGCMTRSWAGGRRVSCCFSNG
                     SSERTRAQSEYGVYVVANQGKASRYEFACKLAELASCDGSLVRPGSREGKPARRTKNT
                     TLDCARLKRDFGFALRPWEDALSECVQSMGLFSQNMLRQL"
     gene            17775..>18067
                     /locus_tag="PLM83_00565"
     CDS             17775..>18067
                     /locus_tag="PLM83_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOK69938.1"
                     /translation="MKPRVYVTRRLPQEGMDMLEGGCEVGFNAHDRGMTREELMACVK
                     DADGLVTLLSDSIDAEVMDAGPKLKVIANYAVGFNNIDIAAATERGIAVTNTPG"
     assembly_gap    18068..18077
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4344 a         4322 c         5261 g         4467 t
ORIGIN      
        1 tcccataaat accttccata tcgctatctg ttaccggatt gcgcaaatgg cggtcacgtg
       61 gcataatcgt ctagaaaagg tggtttgcta catgctcaag agaatcaacg cattgtttgg
      121 cgcacaagat atgaccgagg gaaagccaat gtccgtcttg ctgcggtttt cgatcccctt
      181 gctcatcggc aactttgctc agcagatgta cagtactgtg gattcaataa tcgtcggtcg
      241 ctatgtgggc gacaaagcgc tctcatcaat tggaacgaca ctccccgtca taaacttgct
      301 cctcgttctc ttcatggcag tttcaacggg cgcagggatc atggtcgcgc aatactatgg
      361 ggccaaggat aaagaatcgc tttcgcgttc aattgggaat gcgatgacat tggttttcat
      421 atcatccctt gtgattatgg cggttggcat acctctggcg ggacagctgc tcacattgac
      481 caaaacccca gtagaaacat ttgacatggc ctactcctat ctaaccataa cgctttcggg
      541 gattctggga gtcggctatt acaatatagt ctccggcatc ctgcgcggac tgggcaattc
      601 cgttttccca ttgcttacac tgctgttggc atcgattctc aacatattca tggacatatg
      661 gtttgtagcc ggattgggca tgggagttgc aggcgcagcg tgggcgacta tcatatccca
      721 ggccgtctcc gcagtgctat gcattgcgaa gctccggaga atgaaagatg ttgtgaacct
      781 caacgcttcc aatttggttc ccgacaagaa gctgaccggt caattgctgc gattgggcct
      841 gccggcaggc atcacgcagg cggttttctc gatgtccatg gtatttgtgc aggcccttgc
      901 caacagcatg ggttaccagg tggtcacgtg caatactgcg gtcatgcgca ttgacggctt
      961 tgcgatgatg cccaacttca ccttcggcat ggcgatagcc acctttgttg gccagaatat
     1021 cggcgcggat aagatggacc gcgttgccca ggggacaaag gatatcctga aagtgagtct
     1081 gaccacttca tttgtgctgg tggcttgttt gttgctgttc ggcgagaacc tgatacgcat
     1141 gttcactaca actgaggaga tcattaacct tggtgtgcgg cagattcgca tactggctgc
     1201 gggttatgtt gccatggcca tttctcaggt ttttggcgga atcatgcgcg gcgccggcga
     1261 caccatgcca tccatgtgga tttcgatgtt tactgttgtg gcagtccgcg tgcccctcgc
     1321 atatatcttg gcatcgctga ccaggagccc agatcatccc gctggatctc ctgacgcgct
     1381 gttcttctcg ctcctcataa gctggatcgc gggagctgtc tgcaactatc tctggtatcg
     1441 tcggggcaca tggcgcgaga aatcccttgt ggcacatgag caggcagcat gagggatcaa
     1501 gaaactagcc aacggcaata tcgcctgaaa ttaacgatat aatcgacata cactgtccga
     1561 tcttttgctt cgctctggtg catactgcag tttagttttg gcaagaaggc gtggcagacc
     1621 agcgaacgtg tatttattgc taaaatttcg gcttagcctc ttgctaattt gtgccgatgc
     1681 tgcatgctac tgatggagtt ccgtaattgt gcggcagtat atcaatatta gtctggttct
     1741 tgtatactag tgctggaatc atggcacatg tttgccaaat tacctcctca gaatttgagg
     1801 cggaaaggag aatgtgaatg ggacatcggc gacgaagtgt gttgttcgca gttttcttgg
     1861 cagtgcttat gattgctttt gccacaacgg cgcacgggaa tagcgcggtg ggtgaggcgg
     1921 ggcctggaaa tccgactgag ataggtgttg acgagaagga aatggtttcg gattcgaaag
     1981 cggaagctac ggccaagcgt cctttggtta ttgcgcacag aggagcgtca ggggtagctc
     2041 ctgaaaacac gatggctgcc tttgagcgcg cagtcgagat gggcgctgac atgatcgagc
     2101 tcgatgttca tgcaacaaaa gatggccaga ttgtggtgat ccacgatgca gtgcttgacc
     2161 ggaccacaac tgggagcgtc agggggtccg ttgtttggcg cacccttgag gagatcaaga
     2221 cgctggatgc agggtcctgg aagggagctg aattcgcagg ggagaaggtt cccacgttgc
     2281 acgaggtgct ttcgacgttc aaagggcgta ctaaggtatt gattgagata aaggcgaagg
     2341 gcatagagga caaagttgtg gagatcataa gagagaccaa aacggaggac gatgtcataa
     2401 tacagtcctt cgacgtcgaa gcagtgaggc gttgccgcaa cctattgcct caagtaccgg
     2461 ccggcgttct gttcagcgcg ccattgcttt ttggaaagtc atctttcggc gagaaggtta
     2521 tctccacagc actcgatgtc aatgcggact tcgtggcggt tgaccagggg ggagtcacgc
     2581 cggagctggt tggtatggct cgagagaatg ggcttggggt atttgtctgg actgtaaaca
     2641 aaacaaagga catgaccaaa atgatacaac tgggtgtgga cgggataata actaatcatc
     2701 cggatgttct gatcgggctc ctcgctgagt aatatgcgtc atttcggcgg gtaattccag
     2761 gtattttctg tccggatgaa attggcgcga atatgctgca aataatgtga tttgatagaa
     2821 gccattggtt cttgccttgc cggtgatcgg gttctgcatt tcgatgccga tggagaaccg
     2881 atggcttcgc gtttcctgca ttgctggtca gacatctctt ggcagcgtgt ggaggcttgg
     2941 catttcgggc aaatcaaggg agctatgaga ggaggcgaat ggtattagct tcgggatagt
     3001 gacggattca tcggcaaacc tcacggatga gatgattgat aagtacggga ttgatatcat
     3061 ttcgctgtca tatagaatcg gggaagagga atgccagagc tatgtaaagg gcgagaagac
     3121 cgatctcaag cagttctatg agaaactgag acaaggcgag gtggccgaga cttccctcat
     3181 aaacatgagg gattgcgaga atgcattcga aagcgttcta caggaaggca aggacttgct
     3241 ctacatcggt ttttcatcgg cgctcagcgg gacatacaac gccgcgtcca tagtagcgga
     3301 caatatgcgg gagatttatc ctcaacgcaa gatttatgca attgactctc ttgctgcgtc
     3361 aatgggtcag ggattattgg tttatcatgc tgtagagcaa aggcttgccg gaagatcgat
     3421 tgatgaggtt aaggactggc tcctcgagaa caggcttcga ctttgccatt ggtttactgt
     3481 ggatgacttg atgtacttga agcggggcgg aagagtatct gtctcagctg cagtgatagg
     3541 gacggtcttg tcagtaaagc ccgtgatgca tgttgacgac gaaggaaagc ttgttcctgt
     3601 aaggaatgtt cggggtagga agaagtcgct agtggccctc gtggatgaga tggagaagac
     3661 ttgcatagac cctacggagc agatggtttt catatcccac ggcgattcac tggaggatgc
     3721 cgagtgcgtt gagaagctcg tgagggagcg tctgaacgtg cgggatgtca ggattaacta
     3781 tgttgatccg gtgataggcg cccattcggg gccaggaact attgccctgt ttttccttgg
     3841 cacaaagaga tagacttttt tatcatctgc ggatagggcg caccagagat tcctggagca
     3901 caaaaaggtt tcggagagga ttgctctgcc gcgcggcaga gcaatcctca cgagcacgtc
     3961 attcaaggga tattccagcc gacatccagt atggcggttt gcatgtctgg caccgctcat
     4021 atctgcgcca catggcttcg gcgagggtca tgggaatgcg gcttgaagcg aggtatcggc
     4081 agccgtcgcc atgatatttc ttgcctgtat tggtcacata tacgatgatg tgaatccatc
     4141 ttctcatggt tccccagatt cctcggcctt ccatggtggc ctcatgctga aaaccgacga
     4201 aaaccgctgc atactttgta ttgggcataa gcatgtatgt gtacgcgtag ccttccagga
     4261 gcaaaatggc gttgaacatg cagtctctta tcttttcctc cgcaaagtcg gctggcggtg
     4321 cagtccatac atatgcgagc agtcgaccgt atttgtcgag tgtgcccata tcagtttcga
     4381 gccagactct ccttccttcc agcagttctc tggtgaaggc ggctgccgcg aatgcataat
     4441 cacggaccga ggggtctttg tcggtcaact cagggcagtc cactcctatc agccgcactc
     4501 tctgccgtgc gccgttctcc agaatcactt ctaccgtgtc tccatctagc acacgaatga
     4561 tggttgccgg catcagccgc tgctgttcct ccgcgctgag cgcgtagggg gatgcaaaga
     4621 gtccaataag gacgatcatg gccacaaacg tgcaccgcct catttccggg cctcctctgc
     4681 attccaaaga atcagcggct agttaacaat cagattcaag gaaagcgggg agacgtcctc
     4741 tcgtgatatt cagtccggct acggcgagaa atctgtggat tatgaggata gtcgtgtaaa
     4801 ggaaaagtct ttttcgaaga atatgacaat gggaactaag attccgaact tagcgtgttt
     4861 cagacgtatt atccctgagc gcccagttaa ccagtctact gtatcgctca tgggtgaaga
     4921 aaaggcagac actgtgacgc gataaggttc gcgcaaggct tagccgaatc atgagccttc
     4981 cgattcgcgc atattgccag tcgcttcgag ttctgtgctg tcaggtttgt cggcgattta
     5041 aatatgccaa tgcgatcgtg ggacatgaag gcgggggaag gcccaagcgt tcaccggatg
     5101 accggctagt gggtcacccc ggcctggcga aacgaagagg gaaacgatgc cctagctcct
     5161 ctcacctggc atgcgctggc caaatcggtc ttgttctaca ggaaatcatg tcatggcaaa
     5221 agctctattc agtgaatggg cgggaagata tgaggaggcg aattgcgcat gaatgcagat
     5281 tatggagcct ttggaggagg ggcttgttcg gctgcgctgc ggtgaacttc ggcaatgctc
     5341 atgctgtttg catgtgcaaa gcgccgctaa ctggccgctg cgactgaagc tctcctgtct
     5401 ccactcacgc cacgcggtta cttgagatct gtttagaatg ggcgcctgtg tcccttactc
     5461 tgtaaggatt gtggatttct tctattgccg gacccgtatg ttcgtcttat tgcatgcgaa
     5521 tcctgttcga cccatccatc gctgatggta ccaaagtagg ggctgggttc tcgatgcttg
     5581 ccgttgttta cggcgtgtcc ggttaagaag agtctgctcc gcgcccgtgt aagtgctaca
     5641 tagaagagcc tcagctcttc gtcctggcgt ccgtctttta ccgatcgata gcctgggaat
     5701 tcatgttccg acagccctgc tacgaacact acatcgaact ccagaccctt ggattggtgg
     5761 accgttagaa ttcggacgcg ctcgtcttct ggatcaaccc tgtccacgtt ccgagccaag
     5821 gcgacgaagt ggagtatgct ctctaaggaa gagataggat cgaggcgtac gtcatccttg
     5881 tctcgaaaga tcctcacaag ctctctcagg ttttccatgc ggcggggctc tttgatgtag
     5941 taggcattca aaccagacct ctccagcact tcgtcgagga gagcgtcggg acgaactctt
     6001 tctgctaaca tcaacatttc ctcgatctct cttgcaaggg gcacaaacga ttcgcccgtt
     6061 tcccggacag cctgcattct gccgatactg cctttgttta catacggggc tagggcgatg
     6121 aggagagccg ttgtggcgtt tacatcgtcc atcgctctgt gcgtaggttt gcaggggatg
     6181 ttgaaatagc gtgtcaggtt ggccagggaa taatcgtcaa cgtccaggaa ccgccgcgca
     6241 atgtcgaggg tgtcggcaca ctggtgcgat ggtgggttga ggccaaggcg tcgagcatta
     6301 gcccgaaaca tgcgtacgtc gaaactgacg ttgtggcccg cgagcagtgc gccatctgca
     6361 aaggccatga actgcgacag ggtctccaag gggtctgctc cgtgggctgc aagaaactgg
     6421 tctgaaagcc catgaatgtg cctggaatcg cccacgctca cgcgattgcg caggtagcgg
     6481 tggaatgtgg cgactggttg tccatgttcg aggcggatag cggctagttc tacgatatcg
     6541 tcggaggcgg ggttcagccc ggtagtttcg gtatcgaaga ctacgacaga tccggaatcg
     6601 agttccctca taagccgtgc gaacgggtct cccaactcga tagtggatgg attgaccatg
     6661 tcgatcagcc tcaggcctgt tctttcggct tctatgacac tatcgatggt ttggcgtccg
     6721 atgtttcggg gaggcctaag aagcatccgc tgaaagcttc gaccgtcgaa tgggtttcgc
     6781 agaaacctca ggtaggcaat ggcgtctttc acctcttgcc tgcggaagaa ttcaaattgc
     6841 tccacggtga ggtggggaat gccggcctct gcgaaagcct ccgagatcac tgttccgcgc
     6901 cagttcgttc tggttagcac ggcgattctt ccggggcgaa gtgggtcaat gttgttggcg
     6961 ctttcgcccg gttctatcag tttgcgcgtc tcctctgcta tccattgggc ttcggcttga
     7021 gaatcgcggg cgaagtggac tactatcggg tttccttcct ctgctgtggc gcacggacgc
     7081 actcggtcga agtcactgta gttggcggcg acagaagccg cagcatccac cagtgtgcgg
     7141 gtggttcgat aattggtgac agatgagaag cgccgagcct gtgggaagtc tttttgcagc
     7201 agcgtgatga ttttctctgg ctgagaaccc ctccattcgt atatggtctg atcgaaatcg
     7261 cctgccagcg cgatattccc gtacttcttt gcgagaacac gcagaacctg atattccgac
     7321 acgtgcgtat cctgcatttc gtccacttgt atcatggaga aacgttcggc ccagcggttg
     7381 gcaatgtccg gctcgacaga aagcatagcg cgagcaaagt agacgagatc ggcgaagtcg
     7441 agggcatgat gggcagcaag ttcatcctgg taggcaaggg ccagacgacg ccatgctcgg
     7501 ggcaggctgt cgaagaccgc atcttgcacg gaatttgtgg aaagcacggc atcaggagca
     7561 tttatcttag cagttgtgat cgcatcctgc gcatctctcg cttcatcgtc atagcctgag
     7621 caaacgagat gaacgccagt ccttttcgcg cattgtctga tgatctccat ggagtcgtcg
     7681 tcatcgaaga tgacaaagcc tgatggaaga ctcatctgtt ttgcctcgct cctcagcatc
     7741 cacgcgcaaa ggctgtggaa ggtgcggacc actacgcctc ttgcatgctc tccacaactg
     7801 actgttatcc gttggcgaag ctcctctgcg gccctgttcg tgaacgtgac acacagtatc
     7861 ctattgggat caaccccgtg gcgtatggct tctgcagccc tctcggcaag ggctagggtt
     7921 ttcccggtac cgacaggcgc gtgcagcacc acgcacccat gttggcaatc gacgaacgcc
     7981 ctttgcttgc tgtcgaggac cggcctgact gcgacttcag gcttggggac tcctggcacg
     8041 ctcatgtgta tttccctcct gctctatgga gaccagcatg gtggaagacc gctggcggat
     8101 gtccatgaaa gttttgggtt gctagtgtat agagttcgct ttttgatgca gatggccttt
     8161 tcaattttag agaaggagtt ttgcgaggcg tgtagaatac aaacatcgac cctgtggtct
     8221 gaccataata ccataggatg atatgtccaa aatgaggctg caaggagagg gcgttgtgca
     8281 gaagattgaa cacaaatccg tgagccagca agttgaagag gcgataaaaa gatacatagt
     8341 ccagaagggc ctcagaactg gcgacaggtt gcccactgaa agggaactcg ctgaaatgct
     8401 cggcgtaagc agatcatcgg ttagagaagc aattgtgggc ttgcaggctt tagggatagt
     8461 ggagtccaag ccaaaggtag gtattaccgt gagcgagccc aatgtggcct cgttgattca
     8521 tgcagttgcg ttcaattccg ggctcaacac agagaagatt ttccagctta atgaggctcg
     8581 cctagtgtta gagaccgcgg ctttgaaatt ggctgtggag aaggctgacc agagcgacta
     8641 tgaagctatg gcagaaacca tatatcgaat ggaagcaaaa gttgaagcag gcgaaagctg
     8701 tgcagcagaa gatctcgcat ttcaccaagc aattgtagaa gcaacacaca atccaattct
     8761 gagtcagtct tcccacactt tacaggaatt cttccatatc atctccgcag agggggtagg
     8821 tgccggtgtc agccctgcca gaatcctcag cgagcacaaa gagatttaca atgcttttcg
     8881 acagaaaaat gttggcaaat gtgaagagct tatgacgagg catttacagc gctactactt
     8941 tgctcaacgt caagaagggg atacggaaaa atgacagctg acttcgaact aaaagggata
     9001 attccaccac ttgttacgcc tctcgatgat gatggtgcct tggatgagca ggcattccgc
     9061 agatccatac ggagaatggt tcgagcaggc gcatcgggta ttttcattct cggctccagt
     9121 ggggaatttg cggctctgac agatgcctgc aaggcacggg tgatcgagac tgctgttgat
     9181 gaggttggcg gcaagatgcc ggtgcttgtt ggagtaggag acaccagcac agccagggtc
     9241 ttcgagaaca tgcaggccgc tgcactgccc ggcgtatccg ccatagttgt gtgcacccct
     9301 tactacttcc caacccaaca ggaagcgcta gtcaagttct acgaggacat tgcagaaaag
     9361 agccaagtcc cggtagttct ctacaatgtt ccacagacga ccaagacctg gatcggcatt
     9421 gaaacggtcc ggcgacttac cgacaaccct aacatcatcg ggataaagga tagctccggc
     9481 gatttcgact atcaccaacg tctcatatcg ctggcttcat cagcaagccg attcagtgtt
     9541 ctccagggcg atgaattgtc gataggagcc agtgtgctta tgggcgggca tggagctgtg
     9601 ccggggatag ccaacctgga tccgaaagca tgcgttgacc tgtacgacgc ggctgcaagc
     9661 ggcgatgcag ccacagccgt caagatgcag tccggggtct cgtcgctatg cgaggtatat
     9721 aagcatggcg gttctcctgt gggggcgctc aaggcctgcc tcaagatgat gggggagggc
     9781 tcgggtaaac tgacccatcc gctcgatgac cagaaacccc tggacgattt ggagatacta
     9841 tctcgcatac tgagcgagcg tggtctcatt tagtagattg aagcttggag ggtatgcaga
     9901 gtgtcgcatg acagacctcg aatcggcata atcgggcttg gactgtcgaa cccatacaca
     9961 tttgcgcgga tactcaaggc tgaaggagca gatgtcgcat atgtgtggga ttatcaccgg
    10021 gatgccgccg aggcttttgc cagggaaaat ggctgtgaag cggcgccaac atggcaggag
    10081 cttctgaagg cgccccttga tggggtgctt gtcgaaagca taaacgccga tcactgcaag
    10141 ctggcgaaag cttttgttga acaaggtgtg ccggcctatg ttgaaaagcc tctgagcttt
    10201 gattatgaag aggcaaagga ccttcttgac acggctgtga gcaacgattg ccccattctc
    10261 tcctgcagtc cgctcaggtt ttcgccggta ttccaggaga tcgcgcgaag tgtgagggag
    10321 gggcggctag gactgcccac tttggctcat gctagggtca ttcacactat gcatcatttc
    10381 gctacagacc ccagaaagag atggcatgat tctctgtcgt tgggcggggg tatggttgtg
    10441 gacattggtg ttcatgctgt agagcttgga tatatgatgc ttgggcccgg ggcagcaagt
    10501 gtgtactgtg agacgagcag agtcaagtac aaagacatag aatcggagga tacggcagcc
    10561 attaccatca agtttgcgga tggtgcaata gcggtctgcg atgtggtttg cgcaacgaac
    10621 gagctcgaat actctgtgat ggtcaatggc tcagaagact ccatagacag ccgtgaactg
    10681 tgtgccaagt tatgtaatga aggatatggc acgcccgatg gcatcggcaa cgccttgtcg
    10741 gcatatggcg ggtttgtgga gaccatgaga cagttcctga aatgcataga tcggcgttgc
    10801 ttccccgtac ctccgttgga gatgggcgag atagtgaaaa tcctgagcgc tgcgagggct
    10861 tctgctaagc agcattctcc tgtgctcata tagttgtcgt catccagata ggaaggagaa
    10921 tcgagttatg agataccgag ctgggatcgt tggatgcggc cgcatcgggt ggaaacttga
    10981 ggatgacccc aatcgtccca aaccgtgtac acatgcgggc gcatatgcga gcattcctaa
    11041 cactcagatt gtcgctgctt catcacgaac agacaaaagc ttgaagagct tcggcgagcg
    11101 attcggcgtc gacgcgttgt atacggattt caacgagatg cttgagaagg agaacctgga
    11161 tttgctcagt gtaaccacta acccggagct tcaccgcgac atagtggtca aagccgctga
    11221 gtgcggggtg cgcggcattc tatgcgaaaa gcccatctcg ttgagccttg atgatgcgga
    11281 tgacatgata gaagcctgtc gaaaatctgg cacagttctc atggtgatgc ataacaggcg
    11341 atggaacagc tattacgtga aggctaagga gctgttggat ttgggtgcca tcggggaact
    11401 cgttagcatc tttgggaatt gtcaagggtg caaaccttct cccgactggc tgagcgacag
    11461 agaaggcccc ctgcttcacg acgccactca cctctacgat atcatgaggt ttttcgcagg
    11521 ggacattgat tcagtagttg gcagtgctga aaagcgcaag catctgcact atggcgtcga
    11581 agacacctgc ttctcgcata tgcgcttcaa gaatggcgta tccgctgttg ccttggttaa
    11641 tgagagaacg tcgtatatgc ggtttgacat agaacttcag ggttcggacg gcaagatact
    11701 ccttggacct gatgcggaga tgtggcaggt ggttccgagc aagagagctg cgggctttcg
    11761 ggaacttgag aaggttgaat tcccgaagcc tgaacgagaa gccaatgtat atatggacgc
    11821 aataggcgag cttatacact gcgtagagac tggggagcca agcaggagca cgggggagga
    11881 cggacgtgca gcattggagg ccatcatggc aatctatgag tcgcaaagac gggggaattc
    11941 tcccgttcag cttccactgg gtgtgaggga gtcgttgctt cttgctctga ggagagagaa
    12001 caggctttag acaagatccg gcagacagtc gaggtgaggt ttgtgaacac attgtcggaa
    12061 ctgaggaaat cgcgggagtc atactacttt ttggccccct ccgtcatcat attcctcgtg
    12121 tttgggttga gtgtactcgt tgccaacgta gtgctgagct tctattcttg gtcagcgctg
    12181 accgagccaa gattcatagg gctcgataac tacagaaggc tgtttcatga tgccgtcttc
    12241 agaaaggcgc tcgtaaatac attgtattac tgtgcgcttt atgtagtgcc aagcctgata
    12301 atatcagtgc tcttagcttt cgtgctcaac aagaagctca aggggatgaa cctgttcaga
    12361 tcgatcttct acctgccggt gataacatcc tatgtcattg tcgtaatact ttggcaatgg
    12421 atatatgacg cagacatcgg cttgctcaat tcgattctga acaagatagg aatcgcatcg
    12481 gtgccatggc ttttaagccc caaacttgct ctgccgtctc tcgttctcat gtcgatatgg
    12541 aagaacgtag gttacaccat agttatattc cttgcaggct ttcagcagat tgacgttgca
    12601 cttcatgagg cggcacgagt ggaaggcgcc acagaatggc aggtattcag gcatatcact
    12661 ctgcctcttt tgaggcccac gacgcttttt tccactgtga tgttgataat atggtcgttc
    12721 cagatgttcg tccaaccgta catgatgacc catggaggac ctgccagatc cacaactacg
    12781 ctggtttact acttgtatga aacaggtttt caatactttg acctaggata cgcttcaact
    12841 atgtcagtag tgatgttgat catggtattg ccgatcatca tactccaaag caagctgctg
    12901 cgggcaaggt gagagggtga gagcgatgaa tcgattaaga aaaatgccga tgaaaaactt
    12961 ctggacctac tttatcctag tattgctcgg atcaacaatg attgttccat ttattgcaat
    13021 ggtttcccag tctcttaagc caggttcgca ggtattggag tatccgttca gatggatccc
    13081 tgagaatcca tcgatggaga actataggat gctgtttaag cagaatccga tcatgcgatg
    13141 gacactaaac agctttattg tggcgggcgg aagcacaatt ctgcagctga taacgtgttc
    13201 gcttgcggct tatgcgttct ccaggacgtc atttcccggc aaagacatac tcttttggat
    13261 gatgatgtcc atgctgataa taccgagtca gactacctta gtaccgttgt tcttgctttt
    13321 ttccaaactg aatcttacaa acacttacac ggcattgctg ttgcccacgg cttcgagcgc
    13381 gtttgggatc tttttgctca agcaattcat ggagccaatt cccaaggatt acgacgaagc
    13441 tgcaagtctc gacggctgca ctagatttca gttctactct aagatactgc tccccatgtg
    13501 caagccggca ctagcggttc ttgctacgtt caattttgtg acgcagtgga atgatttcct
    13561 gtatccgttg atcatgactc agaccagaga catgttcacc ctgcagatag ggttgtctgc
    13621 acttcaggcc agcgggtcaa ggggagaaat gggaggaatg ggggtggtca tggcaggagc
    13681 agtgtggggc tttatcccaa cgttcattct atttctgttg ttgcagaagt acttggtcga
    13741 gggagtctcg tcagctggaa tcaagggctg acgtctaaaa gcgctgaagg ggaggaatat
    13801 catactatga agtcgcatgg aaagctcgta agaatgctcc gcgctacgtt gctcacaatc
    13861 tgtgtcctct tgctttttgc tgcgtctacg ggattggctg cacggcagga gacagtgacc
    13921 atgtgggcgt tcccgctatg cagcgatgac atcgaaatgt acagcaagct cgttaatgaa
    13981 tttgagaaag aaaaccccaa tatcaaagtg catgtagagg ttctcccgtg gcagggccgt
    14041 atggagaagc taatggcagc tctagctgcg aaccaggctc ctgatgtgat gtacctcaac
    14101 gtgtttctag tcccgaaact ggcagctacg gggaacctgg cgcccctcga caagtatttg
    14161 ccttccgatg ttagggctga ctacttttca ggcgctatca agtcatgtca gtatgatgga
    14221 cgcctctatg ctatgcccgt acttcagagt gtggtcacgt atatatacaa cgtagacctt
    14281 tttaagaagg caggactcga tcctaaggct ccgccggaga catgggctga gttggaagag
    14341 gctgcgcgca agttgacctg cgacacggac ggagacggca agattgatca gtggggcatc
    14401 agatttgacc tcaatcgtcc gagcccgatt acgagcgtgg ttccgtttgt ctggcaggct
    14461 ggcggagatc tcattagcgc cgatggaact gaggctatct atgacagccc tgaggcagtg
    14521 agagcagtgg agttcattaa aggtcttatt gacaagggct atatgcagaa gtcgaacatt
    14581 caggggggcg gactcccctt cacgtcaggc aagattggca tagatctgca gcgcgagaac
    14641 atggacgtca tcaagctcag agccgaaggc ctcgatttcg agattgcagc cggccctatt
    14701 cttaaggatg caaagaagat aggttacggc actgtgggtt cttatgggat actcggcaaa
    14761 tcgaagaaca aggatgcagc cgcaaaatgg gtgcttttcc tcaccagacc tgacaatatg
    14821 gcctatataa tgaagaacac aggatttgtg gctcccagaa agtcagtatc accggccaaa
    14881 tacgtcacgg atccggtgat caagcaattc gtcgagcagg cgccgaactg ccgaccggaa
    14941 acaccacacc tctatattca agaggtactc agcacgatgc aacctgccat tgagaatgca
    15001 cttttgggct tgaagcctgt tgaggcggca ttgaaggagg gcactgctga ggcaaaccgc
    15061 ataatgcgga ggaagtagcc ttccataagt ggagtgtgcg agacaggaag ggttggcgtt
    15121 tccgggtgga gaaccggcgc tcgtatgtgt gaatgcgtgc taggctatgt gccggggtcc
    15181 gggatcgtca acgagtgaag cccgtctgca tacaaatgac ggcgccgttg cgagacaaca
    15241 ggttctcgcg gcggcgctgt cttgcgtttc ttatgtgact cgcttgtgaa cgccagaaca
    15301 gttatttcgt ggtgtaactt tggagtagga cggggagcaa tgaagaagag agaccacgta
    15361 gtcctggaag atgttcctga gcggggtctc gcggtgacct tgcggaggca caccctgtaa
    15421 gctggcattg cgcctgccga ttaccacgta acaggatttg ctttgggact gcttgagttg
    15481 agagcatcca gaaccatgcg ccacgctttt agggccccgc ctttcataat ccattagccc
    15541 aggttgatta agactcgaac ttgcgggaag gaaaatcgtt acccgaaacg gaagattcat
    15601 cggtatccag ctgttcgcca gagtttcgta tggcgagcga agcgacgccg acaggctggc
    15661 gtctgtatat catgaatgta ttttttgggg gcgggtcaca tgttgctttg tacacacaga
    15721 aagggcttgg tgctttccat cattgccttg ttggcattcg ggtatgggtt gtttggcgca
    15781 agttgttgcg ccggcgccgg ggtgccagtt gcgtcagacg gcggacttgt ggggtcgaaa
    15841 cgcatcacag taatgacata caatatccat tatggagtcg gtttcgactt cgtgtatgac
    15901 ctggatcgaa tagcggatct gatcgaagag gaaggcgccg acattatcgg cctatgtgag
    15961 gtggagcaat gcacaaaaaa attcaattac caagatcagg ccggacatat cgccgataaa
    16021 cttggcttct actatgctta tggccctatt tttgctcgca aggatggcgg ctatttctcc
    16081 aatgcgctga tcagtcggta tcccatcgtc tcccaccaga cgcatcaact gcctatcttc
    16141 aatgacaacc aaaagcgcgc tgttctcgag gctcaagtag atgtggacgg tacgttgctc
    16201 aatgtgttcg tcactcacct ggaggtcaag gatcacgagt cccgcatggc gcaggctcag
    16261 gcgctcctcg agatcgcctc gcgcacagag tgccctcgca tcatcatggg ggatttcaac
    16321 accatcccca catcctcagt gatcgcgttg atgttgcacg agtacaacga tacataccaa
    16381 gtctacaggg tgctcaccga cagcgcggaa attgcatctg aggggctttt tgaaagggat
    16441 tatctcaaag gcggatacac gattgacccg tttgatccca accggcgcat cgacttcata
    16501 ttctcgtcct tcgacatacg agtagtggga gagcctaagg ctgcgcgagt gcaccattct
    16561 atggcatctg accatctccc atacatcgcc acactggagc ttccggcggc gtcgctttga
    16621 tagaaaaagg cgcaaggcgc aggcgagcct cgaggaaaca tgcgatttgc atatgtgcgc
    16681 cggcgcggag tggccctgtg gcaatatgct ggagaaggaa tccattggaa agctggtgga
    16741 gaacatagtt ggggtgttat ttgaatcgta ctaatgcaat cgcgggggcg gtagcttgtg
    16801 agaacagtag tgactggagc aacaggcctg ttgggcgaag aggtcgctga ttggttggca
    16861 ggccaagagg gacatgaagt gattggactt tgcgggagac gtgatcttga cgtagttgat
    16921 acccgggcag ttgtttcctt tatgtgcagg gcccaacctg atttgatcgt ccactgtgcg
    16981 gcagagcgcg acatggatgc agctgagcgc gatccaaaag ctgcctacat gcttaatacc
    17041 tttggaactt ggaatctggc gctcgccgca aggctgtgtg attgtaaaat ggtcttcatc
    17101 agcagcgaag cggtctatag tggcgaaaag gatgctccat atacagaatc tgaccttgct
    17161 gctcccttga gtgtgtacgg atggagcaag ctagctgccg aggaagaggt caagtcgttg
    17221 cttgagaggt atttcatcct tcgggttccg ctgctgttcg gagcgaaagg gcacagtgac
    17281 aataaccgga tactcaactt tgtaagggct ttgcgtagcg ggaaatgtgt gacagctccg
    17341 attgaccaat ggacatcgcc cacgtatgcc cgggatgttg ctatggccat cgggtgcatg
    17401 acgcgctctt gggccggtgg gcgccgcgtt tcttgctgct tttcgaacgg gtcttccgaa
    17461 agaacacggg cccaaagtga gtatggggtt tacgtggtgg caaaccaggg gaaagcgtcg
    17521 cgttacgagt ttgcgtgcaa gcttgcggaa ctggctagtt gtgatggatc tctcgtgcgt
    17581 cccggaagcc gcgagggaaa gcctgcgagg cgaacgaaga atacaacttt ggactgcgcg
    17641 cgccttaaga gagatttcgg ttttgctctt cgcccatggg aggatgcgct tagtgagtgt
    17701 gtccaatcga tggggctttt cagccagaac atgttgcggc aactataggg caattgcaga
    17761 ggagtgagat ggatatgaaa ccaagagttt atgtgacgag gcggctccct caggagggca
    17821 tggatatgct cgaaggcggt tgcgaggtcg gcttcaacgc gcatgacagg gggatgactc
    17881 gcgaagagct gatggcgtgc gtcaaggatg ctgatgggct ggtgactctt ctcagcgaca
    17941 gcatagatgc tgaggtgatg gatgccgggc cgaaactgaa ggtcatagcc aactacgcgg
    18001 tggggttcaa caatatagat attgcggccg ccacagaacg gggaatcgcg gtgaccaaca
    18061 cacccggnnn nnnnnnntgg aagtagctgc agccggaggg cataatattc taatgttata
    18121 tatggtttta tagtataaag ccttaagcga aaatgaatag gactgtataa taaggttaag
    18181 gctttatatc gcccgaagcg taaaaacgca ataggaagaa taaaattttt gccggggata
    18241 ggccagcctt gcaaactggc tgacaagcga agtctccccg cttccccggc aacaatttca
    18301 aatagggaga cagaaaggga gacgaaatta tgaaatccat aaaagggaaa gtcattcggg
    18361 acattaggaa ccaggaagca aaaagaaagc aactccaaaa ggag
//
LOCUS       DAOSAI010000007        18171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_2139_length_18171_cov_3.576838, whole genome shotgun sequence.
ACCESSION   DAOSAI010000007 DAOSAI010000000
VERSION     DAOSAI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 18171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18171
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2139_length_18171_cov_3.576838"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..954
                     /gene="rapZ"
                     /locus_tag="PLM83_00570"
     CDS             43..954
                     /gene="rapZ"
                     /locus_tag="PLM83_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOK69939.1"
                     /translation="MAETTISGQELSCIVITGLSGAGKSEAARAFEDLGYFCIDNLPV
                     MLIPKLAELLSETSGRIDKVALVIDVRGQDVFGQLSSVFRTMDDSGFNYKVLFLEASD
                     ETLVNRFKASRRPHPLAPEGGILDGLKLERQLLARLRAGAALVIDTSDLTARQLRAEI
                     VRRFGDNSGAPTIQLTVVSFGFKYGVPADADLVFDVRFLPNPFYVDSLRNLTGNDEEV
                     NEYVMRWPVTKRFLDKLVDLFRFLLPEYEREGKFNLVVGIGCTGGRHRSVVIANQLSR
                     PLRELGYRTHTMHRDIDIGSGDQPASK"
     gene            966..1961
                     /locus_tag="PLM83_00575"
     CDS             966..1961
                     /locus_tag="PLM83_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOK69940.1"
                     /translation="MRHSPVPKPLSRRLRVVALGGGTGLSTLLRGLKEYTNKITAVVA
                     VTDDGGSSGRLRQEMGMLPPGDIRDCLVALADAEPLMKELFDYRFNGQGPLAGHNFGN
                     LFIAAMSDITGDFELAIKESSKVLVVRGCVLPCTLDNCVLGANLEDGNVVMGETSISS
                     SRRAIRSVFLEPATCSPLPEILEAIEAADVIVLGPGSLYTSVMPNLLVSGVADAIASS
                     NAVKVYVCNVMTQPGETQGYTAQDHLAAIYAHAGADIVDWVIVNNSPVSDRVLENYCR
                     EGSIPVAWDCKALESMGVSVVEAPVINQTNVVRHDPEALSKEIMALAVRHVFTGR"
     gene            complement(1948..2478)
                     /locus_tag="PLM83_00580"
     CDS             complement(1948..2478)
                     /locus_tag="PLM83_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HOK69941.1"
                     /translation="MMQLSSLPYLLIPLFLLGIPAYGASKGIDVYACFIEGASDGLKV
                     AAKIAPAMIAMFLVVGLLRDSGALDILAQAVGPATMKLGINPDLVMLAILRPISGSGA
                     LSLLANILKDLGPDTFDGKLASVIQGASDTSLYILALYFSSVNVSKWRHSAVAALLAN
                     AVGFVAAIIACNIIFR"
     gene            complement(2475..3092)
                     /locus_tag="PLM83_00585"
     CDS             complement(2475..3092)
                     /locus_tag="PLM83_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOK69942.1"
                     /translation="MSIMWLLMLASGAIVAIIVKGPASVTSIALSSTREAVQLCIGLA
                     GSIALWSGIAKIAEESGLMSRLADLMQPIIAPLFPGISPRSHAIGYMATSMAANLLGL
                     GSAATPFGLRAMEELAAEDKRHSGKASLRLPAQHQEATDAMCTFVIITASSLTLVPTT
                     VLSLRAELGSADPGSIIGATIIATSCSTGAAIILDRLLSRRRRRR"
     gene            3282..4223
                     /gene="whiA"
                     /locus_tag="PLM83_00590"
     CDS             3282..4223
                     /gene="whiA"
                     /locus_tag="PLM83_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOK69943.1"
                     /translation="MDFYSRMKDELIRVMPTQRCCQVAELSALVKTDGVIQISQGRIS
                     VLIRESNAGVARKVVMLLRDVGRPDTQIAVRQGSGLPRGNTYIVLISDHDQAMELLEL
                     LGIYGEFGLASGIDSAIVANRCCMRAYLRGTFLGAGYMGEPEKGYHVEMALKYRTHAD
                     DIVELLRQFHIRAGIVQRRERSVVYIKHGDDVVEFLRVIEAANALLELENTRIVRSIR
                     NDVNRLVNAENANVSKVVEASVRQIQDIQLIDEMMGIGRLPPALREIAWLRLEYPEAS
                     LTELGTMLSPKLSKSGVNHRMRRISEIANRLRGQQES"
     gene            4241..5668
                     /gene="rpoN"
                     /locus_tag="PLM83_00595"
     CDS             4241..5668
                     /gene="rpoN"
                     /locus_tag="PLM83_00595"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOK69944.1"
                     /translation="MSFVLVQQQVQKLVMTPELRQAIHLLQLSTVDLAEYVSNELLEN
                     PLLEEEGHVGMVVDKQVQAGPEDAVHESDANPEDDENEIDWERFFEDGTDVGWTRGPI
                     DPNVDFDPIPLVKWEPSLEEQYHMELRLACSDMRTLRIGEYLLGCIDGDGFFRGDVHR
                     VAASLGVEIDDVERALSLIQSFEPVGVGSRSIRECLDLQICASGVEDNLCRLARKIVS
                     CHLEDIAAGRMVKVSSELGVDVRQVQAAVDFIRTLNPRPGAGFTGSGETRYIVPDVFI
                     EYVEGEYIVIPNDSAIPRLMINEKYRSMLRDANVDSAAKDFIKEKLDSALWLVRSVEQ
                     RRSTIVKVSESIVRHQRDFLDRGILHMRPLTLREVANDIGMHESTVSRATAGKYAQTP
                     RGVFELRFFFDSGVSTSDGDAAASASVKKRIGEMIASEDPKAPFSDQQIADALGCEGI
                     MISRRTVAKYRDEMMLPPSTKRRRY"
     gene            5762..6823
                     /locus_tag="PLM83_00600"
     CDS             5762..6823
                     /locus_tag="PLM83_00600"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOK69945.1"
                     /translation="MSSEMRMLSAAEIVSLQRRVMPEFSDRIILRYNILRHVSHAQPI
                     GRRALAQSLGESERHVRREENVLRTAGLLVVGPDGIRLSEDGEDILRDLAEYVGLLVG
                     AQRLADLLQHEFDLERAIVITGNSDEDEAVKKEMARVGACVLRDMISDGDVVAVCGGT
                     TMAELAAQTVPASGKRNVVVVPARGSLGEDVEKQADTVAALMAKKLGGVYRILNLPDD
                     LGHELAASVAEEPRTKEVLDTIRQSRIVVHGVGTAVEMARRRNMSPSEIEEIASLGAV
                     GEAFGFYFDRDGEVVYITSSLGLRVSDLSAVETVMCVGGGSSKGQALAAVLRNGFSHI
                     VVTDEGAAREALGKTVDDA"
     gene            6928..7932
                     /gene="gap"
                     /locus_tag="PLM83_00605"
     CDS             6928..7932
                     /gene="gap"
                     /locus_tag="PLM83_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOK69946.1"
                     /translation="MAVKVGINGFGRIGRLVLRAADGHDDIEVVAVNDLMDARTNAHL
                     FKYDSVHGTYPGKVVADDDCIEIGEKKIKVFAEKDPAKLPWGDLGVDIVVESTGVFRS
                     KDKASAHLAGGAKKVIISAPAKNEDITIVMGVNEDKYDHANHHIISNASCTTNCLAPV
                     AKVLMDTFGIKRGLMTTVHSYTNDQRILDLAHKDLRRARAAGMSMIPTTTGAAAAVAL
                     VLPELKGKLNGFALRVPTPNVSVVDLVVDLEKKATVDDVNDALRAAAEGPLAGILDYT
                     EEELVSHDFNHNPHSSIVDGLCTMVMEDDMVKVIAWYDNEWGYSNRVVDLASFIAKQM
                     "
     gene            7962..9167
                     /locus_tag="PLM83_00610"
     CDS             7962..9167
                     /locus_tag="PLM83_00610"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOK69947.1"
                     /translation="MRKLTVEDINVAGKRVFVRVDFNVPMDAQRNITDDRRIAAALPT
                     IEYLLDHGAKVILASHLGRPKGKYDPSATMDPIAERLSELLGKPVRKLGDCIGPEVVQ
                     AVGEMEPGDVVLLENLRFHREEEANDPRFAEALASLADVYVNDAFGTAHRAHASTEGI
                     AHYIPGVAGFLMKKEIEIMGKAIEDPVRPFIAVLGGAKVADKLGVIDNLLDKVDGLIV
                     GGGMAYTFLAAKGYEIGKSILDAERLDYARGAMAKAKDRGVTLELPVDIVAAERFDAD
                     SPYKIVDADGIPADWMGLDIGPKTVERFSNLIRSAGTVIWNGPMGVFEMPAFSRGTRG
                     IAEALASSDAVTIVGGGDSAAACEQYGLADKMTHVSTGGGASLEFLEGRELPGVAALR
                     DEGDMEGLK"
     gene            9164..9934
                     /gene="tpiA"
                     /locus_tag="PLM83_00615"
     CDS             9164..9934
                     /gene="tpiA"
                     /locus_tag="PLM83_00615"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOK69948.1"
                     /translation="MRRSIIAGNWKMNKTIPDGVALGGALADDAARFIEKCDVVLCPP
                     FTAIKAVADRVAESGIDVGAQDVHWETSGAFTGEISCEMLLDAGCRWVIIGHSERRAM
                     FGETDDTVNRKMKAAVAAGLKPIVCVGETLAQREDGLANDVVRRQVRGAFAGLSAEDA
                     SKAVVAYEPVWAIGTGRASTGEDANAMARVIRDTIAELYSRDLSEAVRIQYGGSVKPG
                     NMAEFARQPDIDGALVGGASLKAADFMGIIAAMIDSKE"
     gene            9980..11263
                     /gene="eno"
                     /locus_tag="PLM83_00620"
     CDS             9980..11263
                     /gene="eno"
                     /locus_tag="PLM83_00620"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOK69949.1"
                     /translation="MSIITNVYAREILDSRGNPTVEVEVTLFDEVVGRASVPSGASTG
                     QHEALELRDGDRDRYMGRGVTLAVDNVNGPIADEIIGMDAREQAIIDATMIDLDGTPN
                     KENLGANAILGVSLAVAHAAAEFFGLPLYQYLGGVGARTLPVPMMNILNGGEHADNNV
                     DIQEFMIVPVGADSFSEALRMGAEIYHSLRSVLKKRGLSTGVGDEGGFAPNLSSNEEA
                     IAVIVEAIEKAGYGPGEQVYIALDVAASELYRDGKYRLEGEGVEYNSEQLVSYYQALA
                     DKYPIISIEDGLSEDDWDGWKIATERLGGKIQLVGDDLLVTNVERIEKAIERHVANSV
                     LIKVNQIGSLTETLDAIEMAKRAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGAP
                     CRTDRVAKYNQLLRIEDQLGDAAVYAGLDAFYCIR"
     gene            11302..12348
                     /locus_tag="PLM83_00625"
     CDS             11302..12348
                     /locus_tag="PLM83_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOK69950.1"
                     /translation="MHGITEVLGVLVGHAQDDDALTGCTVVLTPNGAVAGVDVRGSAP
                     GTRETDLMRPCNLVERIQAVVLSGGSAYGLDAASGAMQFLEEEGVGFETPFGVVPIVG
                     AAVIFDLGIGNPKVRPDRSMGYRACRAASSGACAEGNVGAGAGATVGKMLGDGYAMKG
                     GIGTCVAELPQPGRRKPVHVGAIVAVNALGDIVDPWTGNVVAGAYDRAEKRFIGPGAA
                     RRAGRSGRGPMDVYTAKDPEDLAGMNTTVAVVATDAMLDKEGANKVAGMAHDGLARAI
                     VPAHTMYDGDTVFCLSTGKRSLSRDRAADITAIGSLAAEVLAAAVVRAVRASRSVDGY
                     VAACDVAEVASDLD"
     gene            12445..12954
                     /locus_tag="PLM83_00630"
     CDS             12445..12954
                     /locus_tag="PLM83_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOK69951.1"
                     /translation="MKVKTEQIAVSGVLAALTVVLGATGVGFVPLPTPAGAATLMHIP
                     VILAGILEGSGIGSMVGLIFGLFTLRFMGDWRVVIPARLLIGPVSYWVYVALKKKPWS
                     LPLAAAAGSITNTVGTLALAVTFGYIPLAGKAGAISIAVMQGVPEAIVSALLIPPIVM
                     AVNKAMHRV"
     gene            13119..13337
                     /gene="secG"
                     /locus_tag="PLM83_00635"
     CDS             13119..13337
                     /gene="secG"
                     /locus_tag="PLM83_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOK69952.1"
                     /translation="METLLIVLEFILSILLIVVVILQQSKGEGLGAIGGGGQLFFGKS
                     KAAEAFLDKATTWLAIGFLIVSFLFVFV"
     gene            13460..14011
                     /locus_tag="PLM83_00640"
     CDS             13460..14011
                     /locus_tag="PLM83_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOK69953.1"
                     /translation="MTRDEALCVVKANVRNGNLVKHMIACGACMKALASRFGEDEAKW
                     ELAGLLHDIDYDSAGSDPSRHSLEGAEMLDGMGVDHDVVYAVKVHNDYHGLPRKAPMD
                     CALYAVDPLTGLIVASALIHPSRKLAAIDTDFVINRYREKGFAKGARRDQIACCDELG
                     MDLREFIGVCLGAMCDCADELGL"
     gene            14048..14515
                     /gene="smpB"
                     /locus_tag="PLM83_00645"
     CDS             14048..14515
                     /gene="smpB"
                     /locus_tag="PLM83_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOK69954.1"
                     /translation="MAEKGSSVKLVATNRKARHEYFIEETYEAGIALTGTEIQSVRQG
                     RVSLADAYAEVRNGEVFLINAHIAEYDFGNRFNHDPRRPRKLLLHKKEIMRLDSRVRE
                     RGYTIVPLKMYIVRGYAKVEIALAKGKKTWDKRRDIAERDARREMDRAGKARR"
     gene            14618..14968
                     /gene="ssrA"
                     /locus_tag="PLM83_00650"
     tmRNA           14618..14968
                     /gene="ssrA"
                     /locus_tag="PLM83_00650"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            15286..15897
                     /gene="prxU"
                     /locus_tag="PLM83_00655"
     CDS             15286..15897
                     /gene="prxU"
                     /locus_tag="PLM83_00655"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259907.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:15427..15429,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOK69955.1"
                     /translation="MQARIGKPAPDFETGAFVAGEFKKVKLSNYRGKWVVLCFYPGDF
                     TFVUPTELAAVAVKYPELQSMGAEVLAISVDSKFSHKIWQETELSKMVAGGVPYPILA
                     DQGGKIGTLYGVYDEEAGVNVRGRFIIDPDGVIQAMEVLTPPVGRNVSELVRQVNAYQ
                     HHRATGDVMPSGWEPGKPTLKPGPSLVGRVWEVWKPEQAFGKR"
     gene            17346..>18171
                     /gene="rnhC"
                     /locus_tag="PLM83_00660"
     CDS             17346..>18171
                     /gene="rnhC"
                     /locus_tag="PLM83_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOK69956.1"
                     /translation="MKEERVTEASISSLRRLAKERGWTMEERDIEHGRQARVSDGENK
                     ATIAFYTTGKALIQGRPSSLTEELDSWWAGSHLNRSAYVPPPNSGHGSDANKPSYSGR
                     ARARGVARIGIDESGKGDYFGPLVAAGVYVDAQTETELLSSGVQDSKRLTDRRIMELS
                     GLIVEICPHSVVAIGPARYNGLYKKMGNVNSMLAWAHARCLENLLETVSCELAVSDQF
                     GDESYLKNALMHRGRQITLEQRPRAEEDVAVAAASILARAEFVRRLESLSQQAGVEL"
BASE COUNT         4232 a         4075 c         5610 g         4254 t
ORIGIN      
        1 cagtgtgaca acagcaatcg atgatcatca ggggatgaga gcatggcaga aactacaatc
       61 agtgggcaag agctttcttg catcgtaata actgggcttt cgggagccgg taaatcagaa
      121 gcggctaggg cttttgaaga tcttggctat ttctgcatcg ataaccttcc ggtcatgctt
      181 attcccaaac ttgcggagtt gctttccgaa acatcgggtc gcattgataa agtggcgctt
      241 gttatagatg tgcgcggaca ggacgtcttc ggccagcttt cttcagtttt ccgtactatg
      301 gatgattcgg ggttcaacta caaagtgctg ttcctggaag cttccgatga aaccctcgtc
      361 aatcgtttca aggctagtag aaggccgcat ccattggcgc cagaaggcgg aatcctggac
      421 gggctcaagc tggaaagaca gttgctagct cggctgcgcg ctggggctgc actggtgatc
      481 gacacgtcgg atttgacggc taggcaactg agggcggaga tcgtacggag attcggagat
      541 aattccggtg cgccgactat acagctgact gtagtctcat ttgggtttaa atatggtgtg
      601 cccgccgatg ccgatcttgt gtttgatgtc agatttcttc ccaacccgtt ctacgtggat
      661 tcgctcagga acctgacggg aaacgatgaa gaagtgaatg aatacgtaat gcgatggccg
      721 gtgacgaaga gattcctcga taaactagtt gatttattta gattcttgtt gcccgagtac
      781 gaaagggagg gcaagttcaa tctcgtcgtt gggattggat gcacgggagg acggcatcga
      841 tctgttgtta tcgcgaatca gttgtcgcgg cctctgcgcg aactcggata ccgcacgcat
      901 acaatgcata gggatatcga tatcggcagc ggggatcaac ccgcatcgaa gtagggggga
      961 ctctaatgcg tcacagccct gtgcctaaac ccttgtcccg cagattgcgc gttgtggcgt
     1021 tgggcggtgg gaccgggctt tctactttgc tgagaggttt gaaggaatat acgaataaaa
     1081 tcacagcggt tgttgcagtc acagatgacg gtggaagttc tggcaggttg cgtcaggaaa
     1141 tgggcatgct tcctccaggg gatatccgag actgcctggt tgcgctcgca gatgccgaac
     1201 cgcttatgaa agagcttttc gattacaggt ttaacggtca agggcctctg gcagggcaca
     1261 attttggtaa tctgttcatc gcagctatgt ctgacataac gggcgacttt gaacttgcca
     1321 taaaagaatc gagcaaggta ctggtggtga gaggctgcgt gttgccgtgc accctcgata
     1381 attgcgttct cggagcgaat cttgaggatg gaaatgttgt gatgggtgag acgtcaataa
     1441 gcagcagtag gcgggcgatc cgaagcgttt ttctggaacc ggctacatgc tctccgcttc
     1501 ccgaaatctt ggaggcgata gaggcggcag acgttatagt gcttgggcct ggaagtctgt
     1561 atacgagcgt gatgccgaac cttctcgttt cgggcgttgc tgatgccata gcttcgtcta
     1621 atgcagtcaa ggtctatgta tgcaatgtta tgacccagcc cggggagacc caggggtata
     1681 cagcccagga tcacttggcc gcgatatacg cgcacgccgg cgctgatata gttgattggg
     1741 tcattgtgaa caactctccc gtatctgatc gggttcttga aaattactgc cgagagggtt
     1801 ctataccagt tgcatgggat tgcaaggccc tagagagcat gggcgtcagt gtggtggagg
     1861 ctccggtgat caaccagacc aacgttgtca gacatgaccc ggaagcgctc tctaaggaga
     1921 taatggcatt ggcggtaagg cacgtgttca ccggaagatg atattacagg cgatgatagc
     1981 ggccacgaac ccgaccgcgt tggccaggag cgctgccact gctgaatggc gccactttga
     2041 tacattgact gagctgaaat agagcgcaag gatatagaga gaggtatcgg aggctccttg
     2101 tataactgag gcgagtttgc catcgaaggt atcggggccc agatccttca ggatgttggc
     2161 gaggagactg agcgcgccag atcccgagat tggcctgaga atggccagca tcacgaggtc
     2221 ggggttgatc ccaagcttca tggttgcggg gccaacagcc tgcgccagta tgtccaatgc
     2281 tccggagtct cgtagaagcc ccacaaccaa gaacatcgcg atcatggcgg gcgcgatctt
     2341 tgctgccact ttcaggccgt cggaagcgcc ttctatgaag caagcgtaga catcgatgcc
     2401 ttttgacgct ccatatgcgg ggattcccaa aaggaacagg ggaatcagga gatagggtag
     2461 tgacgacagt tgcatcatct ccgtctcctc cgccttgaaa gcaggcggtc gaggattatt
     2521 gctgcgccag tcgagcaact agttgcgatt attgttgctc ctattatgga gcctggatct
     2581 gcggagccca gttcggctct gagagaaagc actgtggttg gaactaatgt gaggctagat
     2641 gcggtgatta tcacaaatgt gcacatagcg tcggtggctt cttgatgttg cgcggggagg
     2701 cgtagcgagg cttttccaga gtggcgcttg tcttcagctg ccagctcttc catggcacgc
     2761 agcccgaacg gcgtcgctgc gcttccaagc cccagaagat tcgcggccat gctagtggcc
     2821 atgtaaccga tggcgtggga acgtggggat atcccaggga aaagcggggc tatgatcggc
     2881 tgcatgagat ctgccagtcg tgacatgagc cccgattctt cggctatttt cgcgatcccc
     2941 gaccaaagag cgatcgaccc ggcaagccct atacataact gaactgcttc tcttgtcgat
     3001 gacaatgcga tactcgtaac gctggcaggg ccttttacga taatggctac aatggcgcct
     3061 gaagccaaca tcagcagcca cataatgctc attcgatcat ccccccaaaa aaagaatcac
     3121 caatgttttt gctggtcaag tcgcatcgct taaatcgtat tctccttcag tgttcaagta
     3181 tgacgtcatt tggcgcagat ccaagcgtcg cgggaaggag gtaatcggtt gccagctgag
     3241 gaagaatagc agggcgcgct attggcagag gtgatgtttg cgtggacttc tattcgcgca
     3301 tgaaggacga actgataagg gttatgccga cacagagatg ttgccaggtt gctgagctct
     3361 ctgctttggt caagactgac ggagtcatac agatatctca ggggcggatt tcggtgctga
     3421 ttcgggaatc caatgccggt gtcgcccgaa aagtagttat gcttctgcgt gacgtgggac
     3481 gtcctgacac tcagattgca gtgcgccagg ggagtggtct acctaggggc aatacatata
     3541 tagtcctcat cagcgatcac gatcaagcca tggaacttct cgagctcctc ggcatctatg
     3601 gggagttcgg cttggccagc gggatagatt ccgctattgt cgcaaatcgt tgctgtatga
     3661 gggcttattt gcgtggaact tttctaggag ccggttatat gggcgagccg gagaaaggat
     3721 atcacgtgga gatggctttg aaatatcgga cccatgctga cgacattgtc gagttgttgc
     3781 gccaatttca tattcgggct ggaattgttc aacgtcggga gcgcagcgtg gtttatatta
     3841 agcatggaga cgatgtagtg gaattccttc gcgtgatcga ggcagccaat gcgttgctgg
     3901 aattggagaa taccaggatt gtccgatcga tcagaaatga tgtgaacagg ctagtaaatg
     3961 ccgaaaacgc aaatgtttcc aaagtggtgg aggcatcagt caggcagatt caggatattc
     4021 agctcataga cgagatgatg ggtatagggc ggcttcctcc ggctctccgc gagatcgctt
     4081 ggcttaggct ggaatatccg gaggcaagcc tcacggaact tgggacgatg ctttcgccga
     4141 aactgagcaa gtcaggcgtg aaccatcgaa tgaggaggat ttctgagatt gctaacaggc
     4201 tccgcgggca gcaggaaagt tgaggtgaat cgccgtggaa atgtcttttg tgctcgtgca
     4261 gcaacaggtc caaaagcttg tcatgacccc ggaattgaga caggctattc acctgcttca
     4321 actttccacg gtcgatcttg cggagtatgt ttccaacgaa cttcttgaga acccgcttct
     4381 ggaggaagaa ggtcacgttg gaatggtcgt cgataagcag gtgcaagctg gtccagagga
     4441 tgctgtccat gaaagcgacg ctaatcctga agatgatgag aacgagatag actgggagcg
     4501 cttctttgag gacggtacgg acgtgggttg gactcgtggg ccgattgacc ctaatgtcga
     4561 ttttgatccg attcccctgg ttaaatggga accatcgctt gaagagcagt accatatgga
     4621 gctgaggttg gcctgcagcg acatgcgtac tctacggatt ggcgagtatt tattggggtg
     4681 catagacggt gatgggtttt tccgaggtga tgttcatagg gtcgcggcgt cccttggagt
     4741 ggaaattgac gacgtggagc gcgccttatc ccttattcag agctttgagc ctgttggagt
     4801 gggttcgcgt agcatccgag aatgtctgga tctgcagatc tgcgcctcgg gagtcgaaga
     4861 taacctgtgt cgccttgcgc gaaagatagt ctcatgccat cttgaggata ttgctgcagg
     4921 ccgcatggtt aaggtctctt cggagctggg tgtggacgtt cggcaagtgc aggcggccgt
     4981 cgattttatc cgcactctca atcctcgtcc gggtgctggg tttacaggaa gcggcgagac
     5041 gagatacata gtgcctgatg ttttcatcga atacgttgag ggcgagtaca ttgtgattcc
     5101 aaatgactct gccattccgc gactgatgat aaacgagaaa tacagatcca tgttgagaga
     5161 cgccaacgtt gacagtgcag cgaaggattt catcaaggag aagctcgatt ccgcgctttg
     5221 gcttgtaagg tctgtggaac agcggcgttc cactatagtg aaggtatcag aatcaatagt
     5281 cagacatcag cgtgacttcc ttgaccgtgg cattctccac atgcgcccgc tgactcttcg
     5341 agaggttgcc aacgacatag gaatgcacga atccactgtc agccgagcta cggcgggaaa
     5401 gtatgcacag actcctcgcg gtgtatttga actaaggttc tttttcgatt ccggggtgag
     5461 cacttcggac ggagatgccg cggccagtgc cagtgtgaaa aaacgcatag gcgagatgat
     5521 cgcctctgag gatccgaaag cccctttttc tgaccaacag atagccgatg cgctgggctg
     5581 cgagggcata atgatatcca ggagaactgt ggcgaagtat agggatgaga tgatgttgcc
     5641 gccttccacg aagcgtcgaa gatactgagg ccttttttcc atggggcaga aggatttttg
     5701 agggagacac agaattttat ttgacatggg tgggacgcaa tttgactatg cgggacaaaa
     5761 aatgtccagc gagatgcgaa tgctgagcgc tgcagagatc gtttcgttac agcgcagagt
     5821 gatgccggaa ttctcggatc gcatcattct gagatacaat attctccgcc atgtttccca
     5881 tgctcagccc attgggagac gcgctctagc gcagtcgctg ggcgaaagcg aacgccatgt
     5941 aaggcgagaa gagaatgtgc tgcgcactgc aggcttactc gttgtaggcc ctgatgggat
     6001 ccggctgtca gaggatgggg aagatatcct cagggatctg gctgaatacg ttggacttct
     6061 cgtaggcgct cagcggcttg ctgatcttct tcagcatgag ttcgatctgg aacgcgccat
     6121 agtcatcacc ggcaactccg atgaggacga ggcagtgaag aaggagatgg cccgtgtcgg
     6181 ggcgtgtgtt ctgcgggata tgatatccga cggtgatgtt gttgcagtgt gtggcggaac
     6241 tacaatggcg gaacttgccg ctcagacggt tcctgcttcg gggaaacgca atgtcgtcgt
     6301 ggttccggcc aggggaagcc ttggcgagga cgtggaaaag caggctgata cagtcgctgc
     6361 tctgatggcg aaaaaactgg gcggcgtcta cagaatcctc aatctcccgg acgatcttgg
     6421 ccatgaattg gcggccagtg tggcggagga acctcgcact aaagaggtgc tcgacacaat
     6481 caggcaatcc aggatagtgg tgcatggtgt aggaacagca gtggagatgg cgcggaggcg
     6541 gaacatgtca ccttcggaga tcgaggagat agcaagcttg ggggcggtag gggaggcttt
     6601 tggattttac tttgaccgag acggcgaggt agtgtacatt acatcaagtc tgggtttgcg
     6661 tgtgtctgat ctgtcagctg ttgagactgt aatgtgtgtg ggcggagggt ccagcaaagg
     6721 tcaggcgctg gcggcagtat tgcgcaatgg gttttcccat atagtagtga cggacgaggg
     6781 ggctgcgcgt gaggcgctgg ggaaaactgt tgacgacgcg tagcctcgag ctcgcgacaa
     6841 ggacgtcgtg aggaatcttg tccgcggatc aaacgaagaa tcgactaaga aggtggaggt
     6901 gagttccgcc catcggggcg gggaacaatg gcagttaaag ttggaatcaa tggttttgga
     6961 agaatcggca ggttggtttt gagggctgca gacggacatg atgacattga agtggtggct
     7021 gtaaacgatc tcatggacgc ccgaaccaat gcacatcttt tcaagtatga ttcagttcac
     7081 ggaacatatc cgggcaaggt cgttgcagat gacgactgca tagagattgg cgaaaagaag
     7141 ataaaggtat ttgccgagaa ggatccggca aagctgcctt ggggcgatct gggagtcgac
     7201 atagtagtcg agtcgaccgg ggtcttccgt tccaaggata aggcatcagc tcatttggct
     7261 ggcggcgcta agaaggtgat catcagtgct ccggcaaaga acgaagacat cactatagtt
     7321 atgggtgtca atgaggacaa gtatgaccat gccaatcatc acatcatctc caatgcctca
     7381 tgcactacga attgtcttgc acctgttgct aaagtcctga tggacacgtt cggaatcaag
     7441 cgcgggctga tgacaacggt ccactcctat acgaatgacc agaggatcct cgaccttgcc
     7501 cacaaagatc ttcgcagggc aagggctgct ggaatgtcga tgatacccac aactaccggc
     7561 gcagctgctg ctgtggctct agtcttgcct gaactcaagg gcaagctcaa cggatttgcg
     7621 ctcagagtgc caactccaaa tgtctccgtc gttgacctcg tggtggatct ggagaagaag
     7681 gcaacggtgg atgatgtgaa cgatgcgctt cgagctgcag ccgaaggtcc gcttgctgga
     7741 atcctcgact acaccgagga ggagcttgtg tcccacgact tcaatcacaa tcctcattct
     7801 tcgatcgtgg atgggctatg cacgatggta atggaggatg acatggttaa ggtgattgcg
     7861 tggtacgaca atgagtgggg ttactccaac cgcgtggtgg atctggcgtc attcatcgcc
     7921 aagcagatgt aaatcgttgc gtgtgaaggg gctgatgtgc attgcgcaag ctcactgttg
     7981 aagatataaa tgtggcggga aagcgtgttt tcgtcagggt tgatttcaac gttcccatgg
     8041 acgcccagcg taatataacg gacgacagac gaatcgccgc cgccttgcca acgatagagt
     8101 atctcttgga tcatggtgct aaggtgattc tcgcttctca cctgggcagg cctaagggga
     8161 aatatgatcc gtctgccact atggacccga tcgcggaaag gctttccgag ctccttggaa
     8221 agccagtgag gaaactgggt gactgcattg gcccggaagt ggttcaagct gtgggcgaaa
     8281 tggagcctgg ggatgttgtc cttctggaga atctgaggtt tcacagggaa gaagaggcta
     8341 acgatcctag atttgcggag gcgcttgcat cgcttgcaga cgtgtatgtg aatgacgcct
     8401 ttggaacggc gcaccgggcg catgcatcta ccgagggtat agcccattat atcccgggcg
     8461 ttgccggctt cctgatgaaa aaagagatcg aaataatggg caaggcaatc gaagacccgg
     8521 tacggccttt cattgcagtg cttggaggcg ccaaggtcgc tgataagctg ggtgtgatcg
     8581 acaacctcct agacaaagta gacggcttga tagtcggcgg agggatggct tacacgttcc
     8641 ttgcggccaa gggctatgag atcggaaagt ccatattaga tgctgagagg ctggattatg
     8701 cgagaggtgc gatggccaag gcgaaggatc gaggggtcac gctggaactg cccgtcgaca
     8761 tcgttgcagc cgaacgcttc gatgcagatt ctccctacaa gattgtggac gcagatggga
     8821 tacctgctga ctggatgggg cttgacatag gcccgaagac agtggagcgt ttttccaacc
     8881 tcatccgctc agcgggaaca gtcatctgga atggacctat gggcgtcttt gaaatgcctg
     8941 cattttcccg cggaacacgg ggaattgccg aggctcttgc ttcgtcggat gcagtgacta
     9001 ttgtcggcgg aggcgattct gcggcagcat gtgagcagta cgggctggcg gacaagatga
     9061 cccatgtgtc gactggcggg ggggcgtctt tggaattcct tgaaggacga gaactgccgg
     9121 gggttgctgc tctcagggat gaaggcgaca tggagggttt gaagtgagac gaagcataat
     9181 cgctggaaat tggaagatga ataagacgat acccgacgga gtggcgctgg gcggcgctct
     9241 cgctgacgat gcggcgaggt tcatagagaa gtgcgatgtc gttctgtgtc cgccatttac
     9301 cgctataaag gctgttgcgg atcgagttgc ggaatcgggg atagacgtcg gagcccaaga
     9361 cgttcattgg gagacaagcg gtgccttcac aggtgaaatc tcctgtgaga tgcttctgga
     9421 tgcaggatgt cgttgggtca taataggcca ttctgagcgg cgtgccatgt tcggggaaac
     9481 cgacgatacg gtgaaccgga agatgaaggc ggccgttgcg gccggactca agcccatagt
     9541 gtgcgtcggc gagacccttg ctcaacgtga agatggttta gccaatgacg tagtacggcg
     9601 acaggtaaga ggcgcatttg caggcttatc tgccgaagat gcgagtaaag cagtggtagc
     9661 ctatgaaccg gtgtgggcaa taggaaccgg acgggcgtct actggcgaag atgctaacgc
     9721 aatggccagg gtgattcggg acaccatagc tgagctgtat tcgcgcgatc tgagcgaggc
     9781 agtcaggatc caatacggcg gcagcgtgaa gcctgggaac atggcggaat tcgcgcggca
     9841 gcccgatata gacggagcct tggtcggagg cgccagtttg aaggctgccg actttatggg
     9901 aataatcgcg gctatgattg actcgaagga atgacacaaa gggaaaggag cgcaggcgag
     9961 attatctcgc actaggaaga tgtctataat caccaatgta tatgcgcgcg agattctcga
    10021 ctcccggggt aaccccacgg tcgaggttga agtaacattg tttgatgagg tcgtgggacg
    10081 ggcgtctgtc ccatcgggtg cttctacagg tcaacatgag gctctcgagc taagagacgg
    10141 agacagggac cgctacatgg ggcggggagt gaccctggct gttgataacg tgaatggccc
    10201 gatagcagat gagataatcg gcatggacgc acgtgagcag gcgattatcg atgcaacaat
    10261 gatagacctt gatggcacgc caaacaagga gaatctgggc gccaacgcca tacttggagt
    10321 atctttggca gtggcgcatg ctgctgccga gttctttggc ttgccgctct atcagtatct
    10381 gggcggggtg ggcgcccgga cattgcccgt gccaatgatg aacatactaa acgggggaga
    10441 gcacgccgac aacaatgtcg acattcagga gttcatgata gtccccgtcg gcgccgatag
    10501 cttctcggaa gcgctcagaa tgggagcaga gatctatcat tcgctcaggt cagtgttgaa
    10561 aaaacgtggc ctttctaccg gagtaggaga tgagggcggg tttgcaccga atctatcttc
    10621 caatgaagag gcaattgcgg tcatagtcga agcaatcgag aaggccggct acggtccggg
    10681 cgagcaagtg tacatagctt tggatgtggc ggcaagcgag ctttaccgcg atggtaaata
    10741 ccggcttgag ggagagggcg tagaatacaa ttcagagcaa ctagtgagct attatcaagc
    10801 ccttgctgac aaatacccga tcatatccat cgaagatggc ctttcagaag acgattggga
    10861 tggctggaaa atcgccactg agaggcttgg aggcaagatc cagctcgtcg gtgatgatct
    10921 gctcgtaacg aatgttgagc ggatagagaa ggcgatcgaa cgccacgtcg cgaactcagt
    10981 gcttatcaag gtcaaccaga ttggttcgct taccgagact ttggatgcga tagagatggc
    11041 aaagcgcgca ggctataccg ccgtggtatc ccatcgatcc ggagagactg aggatactac
    11101 cattgcagac attgccgtcg caaccaatgc tggccagatc aagacgggcg cgccttgccg
    11161 gaccgataga gtggctaagt acaaccagct tctgaggatc gaggaccagc taggagacgc
    11221 agcggtttac gcaggactcg acgctttcta ttgtattagg taatctgaag ttctgggaga
    11281 ccagtacggg ggagatgcga cttgcatggc ataacagagg tcctgggtgt gctagtgggg
    11341 cacgcacaag atgatgatgc cttaacaggc tgcacagtcg tattgactcc gaatggagcc
    11401 gttgcaggtg tggatgtgcg gggttcagcc ccgggcaccc gagaaactga cctaatgcgt
    11461 ccgtgcaatc tagttgaaag aatccaggcc gtagtgctct caggcgggag cgcttacggc
    11521 ctggatgctg cttcaggggc aatgcagttt ctggaggagg agggcgttgg gttcgaaact
    11581 ccattcggag tcgttcccat tgtgggggcg gctgtaatat ttgacctcgg aatcggaaac
    11641 cccaaagtgc gtcccgatcg aagcatggga tatagggcat gccgagcggc atcgtctgga
    11701 gcatgtgccg aaggtaatgt aggagcgggc gctggcgcta cagtcggcaa gatgttgggc
    11761 gatggatatg cgatgaaggg cggaatcgga acttgtgtag ccgagcttcc ccagcccggt
    11821 aggcgcaagc ccgtgcatgt aggggctatt gtggcagtga atgccctggg tgatatcgtg
    11881 gatccatgga cagggaatgt ggtggcagga gcctacgaca gggcggagaa gaggttcatt
    11941 ggcccgggag ccgccaggag ggcaggcagg tccggacgtg gcccaatgga cgtgtatacg
    12001 gctaaggatc cagaagacct agctggaatg aatacgacag ttgcagttgt ggccacagat
    12061 gctatgctag ataaggaggg cgcaaataag gtcgcaggga tggctcatga cggtcttgca
    12121 agggcgattg ttccggcaca taccatgtat gatggggaca cagtcttttg cctttccact
    12181 gggaagagat cgctatcccg tgatcgagct gcagatatca ctgccatagg gtctttggca
    12241 gcggaggttc tggcagcggc cgtcgtcaga gcagtgaggg cgtctcgttc tgtagacggc
    12301 tatgtggcag cctgcgatgt ggcggaagta gcctcggatc ttgattgacc gagagcgtgg
    12361 cgcaaggttt ggaaaagcag ggtgcgcctt gccgtcttat ttcgtccggc acccgagagg
    12421 ggctggaacg gaggtgcgat gtgattgaag gtcaagactg agcagattgc cgtatcaggt
    12481 gtgcttgcag cgttgacagt agttcttggg gccacaggcg ttggtttcgt gccattgccc
    12541 acaccggctg gcgcagctac tctcatgcat attcccgtga ttctcgcagg aatcctcgag
    12601 ggctcgggga taggatcgat ggttgggctt atcttcgggt tgttcactct gaggttcatg
    12661 ggcgactggc gggtagttat ccccgcaaga ctgctgatag ggcccgtatc gtactgggtt
    12721 tacgttgccc tcaagaagaa accgtggagc ttgcctttgg ctgctgccgc aggaagcatc
    12781 actaacaccg taggaacgct tgcattggcg gttacgtttg ggtacattcc gcttgcggga
    12841 aaggctggag cgatttccat cgcagttatg cagggcgttc ccgaggctat tgtgtctgcg
    12901 cttttgatac cccctatagt aatggcggtt aacaaggcga tgcatcgagt gtagagaatg
    12961 acaatccagg gcctttgcct tggtcaacaa ggcaggccct ggggctttgc attgtatatt
    13021 acggcatgac ctgtaggggc cgaaggagcg gtaccgttgt aaagcaggac ggtgtatgat
    13081 agaatcagtt ggagtcggag cgatgggagg ttgctgcgtt ggagacgctt ttgattgttc
    13141 ttgagtttat cttgtcaata ctccttattg tggtcgttat tctccagcag agcaagggag
    13201 aagggcttgg ggccataggc ggtggagggc agctgttttt cggtaaaagc aaggctgcgg
    13261 aggccttcct cgacaaagca accacctggc ttgcaatagg gtttctgatc gtttcatttc
    13321 tgtttgtttt tgtttaaccc tggcaatcag ggaggcaatg ttataggctg aaacagtagc
    13381 ctcgcacttg agtccgggtg cgaggttttc ccgtatttac aggggccagg ccgcggggac
    13441 agtgatggga gtgacggatt tgacaagaga tgaagcgttg tgcgtggtaa aagcaaatgt
    13501 gcgaaatggc aatctggtta agcacatgat agcctgtggt gcgtgcatga aggcgcttgc
    13561 ttcgaggttc ggcgaagacg aggccaaatg ggagctggcg gggcttctcc acgacatcga
    13621 ctatgatagt gccgggtcag atccatcacg tcattcgttg gaaggagcgg agatgctaga
    13681 tggaatgggc gttgatcatg atgtggtcta tgcagtgaag gtccacaacg actaccacgg
    13741 actgcccagg aaagcaccga tggattgcgc cctgtacgca gttgatccgc tcactggctt
    13801 gattgttgca tctgcgctga ttcatccttc caggaaactg gctgccatag atactgattt
    13861 cgttatcaac cgttatcgtg agaaaggatt tgccaaaggt gcgcggcggg accagattgc
    13921 ctgttgcgat gagctgggaa tggatttgcg ggaattcata ggagtctgcc tgggcgcaat
    13981 gtgcgattgc gcagatgagt tgggactgta atggaagtct tccgtatact gaggggaggt
    14041 gtgtccgctg gctgaaaagg gatcatctgt gaagctggtt gcaactaaca gaaaagcgag
    14101 gcacgagtac tttatcgagg agacgtatga agccgggatc gcgcttacag gaaccgagat
    14161 tcagagcgtt aggcagggaa gggtcagcct ggcggatgcg tacgctgagg tgcgcaatgg
    14221 tgaagtgttc ttgatcaatg cgcacattgc cgaatacgac tttgggaaca ggttcaacca
    14281 tgatccgcgt cgacctcgca agcttcttct ccataagaag gagataatgc ggctcgactc
    14341 tcgcgttcgc gagcggggat acaccattgt tccgctgaag atgtatattg ttcgtggcta
    14401 tgccaaggtc gagatcgcgc tcgcaaaagg gaagaagacc tgggacaagc gacgggatat
    14461 tgcagagcga gatgctagac gtgaaatgga tagggctggc aaggcgagac gttaaaggat
    14521 ggatgtgacc aagaccaatg gaagtgtatg ccattgcaca tatgctgatc gatttgatcg
    14581 agttggcggc cgcgtggtat aatattgtgt aagatatggg ggcgttacgg tttcgacgga
    14641 gagtgctgtg agaggcgcag cgagccgaga tcccacctac tcgttaatcg gtggaagcac
    14701 gataagtgcc aacgattacg cttacgctgc ttaattaact aagtagccgt ccgacccgat
    14761 cttctcccga aagctcggct agggcgtcat ctaagtcggg atgcttgcgt gtcaattctc
    14821 ggaggcacgc actaagaata tcgagatagt ccccgggcag cgtgcccgtg cgcggctcgt
    14881 ggggcgaaaa caagtatgcg ggctacgctc gtagaagtgc ctcaagtggc cttttcggac
    14941 ccgggttcga ctcccggcgc ctccaccatt tttatgcgct tttcggcgct atgaatgagg
    15001 ttcgcagtct tatcccatca gggagggctg cgaattgttg ctttatatgg ggctgagtcg
    15061 atcttgcgac aatacgggat gagcgaatcg attctgcttc gggaaaaatg gccttataga
    15121 cgattgcgtg atgcaagcgg atcacgagtg ggaatggcta gattcttggc ttgtggatgt
    15181 cggcactgct tttcagaata tgttgcggaa atcacaattt ggataatgtg gaggaataag
    15241 cgtgcatatg gagaaagtac cttcgttaag gaggtgtatt gttatatgca ggctcgaata
    15301 ggtaaaccag ctccggattt cgagacaggg gcatttgttg ctggtgagtt caaaaaagtc
    15361 aaactatcaa actatcgtgg taagtgggtt gttctatgtt tttatccggg agattttaca
    15421 tttgtttgac ctaccgagct ggcggcggtc gccgtcaagt atcctgagct tcaatccatg
    15481 ggcgcggaag tgctcgcgat aagtgtcgac agcaagttca gtcacaagat atggcaagaa
    15541 actgagctat cgaaaatggt tgcaggcggg gtgccctatc cgatcctggc ggatcagggt
    15601 ggaaagattg gtactctcta cggggtttat gatgaggaag ccggtgtaaa tgtcagaggg
    15661 cggttcataa tcgatccaga tggagttatc caggcgatgg aggtattgac gccaccagtg
    15721 ggccgtaacg tatctgagct tgttaggcag gtaaatgctt accagcatca tagagccacc
    15781 ggcgatgtca tgccctctgg ctgggagcct gggaagccaa cgctcaagcc aggacctagc
    15841 ttagtgggtc gggtatggga agtttggaag cccgaacagg ccttcggtaa gcgctaggac
    15901 atgacgcagt ctcgcgggga accgtgtcga tgggggccgc aagggcctcc gtcctttccg
    15961 tacattatgg gttgtcacca aacgcatcac gtaagaatgc gctggcacat gctggtgata
    16021 gaaagttaac tatcgtttct agcaactgat tgttcgggat cctaaggcat ctcctagagg
    16081 ttcgcagtat atcttgagat tgttcttgca atcaaatgag cattcaaccg tagatgtgct
    16141 gcgaaaagtc aatagccgat gaacttcaga ctattactgg cggtcagatc cccgttgatg
    16201 taaaaatgcc ttttgtccag gtgaacagtg gatcaccaca tcgttagctc ccctgcttaa
    16261 gccgcatctg agctatctga taacgatgat ggtaatagtg gctccgctga ttttgggtac
    16321 agcgtagccg cgatgataga gcctgaaccc agagtctaca gtaatttttc tgtctgagac
    16381 tacatacgcc ccgtatatct tcttgtttgg tttgctgaag atgtagcttt tcgtgtggtg
    16441 catgctacta cagtcactgt ctcggttccc acctgcacaa caaagtctct gcctttcctt
    16501 taaccagaat ctgtctttat ctagttgtaa accttcttgg tgatgcactc tgctatcaag
    16561 agggcttgca gtcggctttt atcgttcgct tgtatccgat atgtggatga cgatatccca
    16621 aggagtgcga cgccgaagat tgccagcatt atcaggcttg ccatagatat gagcattgcc
    16681 gatccgtgct ggcgacgcaa gagtcggcca tccgcacgcc tccccttatt gcctgataac
    16741 gactgcagta tcggcgtatg cggccttgat tgtgcaagaa tcctttgtct gtttgacaga
    16801 accaagcagt aatctgtatt cggctgagcc gctagcgcgt tggctgagat gtctgtgatg
    16861 atcagtcttc ataaggatta ggccttcggc gtttcatgaa gagcgtctat tgtgcgacgc
    16921 ttctgttctt cctcttgtga tagtcacaga tatcgcccat gtcctattgg gttcgatttt
    16981 agcgggggct acgtccgtgt ctcgactgaa aactccgttg ccggaagcat tgagaagaag
    17041 tgcgacccgt gcaaatcgat ggactcaagg gcaagattgg agcagggccg cgattgttac
    17101 acctagacgt gatcagagcc tcgatgcctc gccttcgggg aatgttatag catgagcaag
    17161 ttattgcgcg ataatcgccc gggtctggct cgcatggtac ctgttaatga aggaatgctc
    17221 ccgaaacgga agatgaggtc gagtggagat agactggccg gtgcggagga tttggcatga
    17281 cgctgacgaa gaaatgagcg cacattcgtt taaccatata aggatagatg taggtggaat
    17341 ctgatttgaa agaagagcga gttactgagg cgtccatctc ctctcttagg aggcttgcca
    17401 aggagcgtgg gtggacaatg gaggaaagag atatcgaaca cggtcgtcag gcaagggtga
    17461 gcgatggaga gaacaaggcg actatcgcat tttacactac aggcaaggca ctcattcagg
    17521 gtcgaccatc ctcgctcacc gaggaactcg attcttggtg ggccggttcg catttgaatc
    17581 gcagcgcgta tgtgccgcct ccgaactcag ggcatggcag tgatgctaac aagccctcct
    17641 attcagggcg tgcgcgcgcc agaggggttg caaggatcgg aattgacgag tcggggaaag
    17701 gcgactattt tggcccactg gtagctgcag gtgtttatgt ggacgctcag acagagactg
    17761 agcttctcag cagtggcgtg caggatagca agcgtctgac ggataggcgc attatggagc
    17821 tttcagggtt gatagtggaa atatgtccac attcggttgt ggccattggc cctgcacggt
    17881 acaacgggct gtacaagaaa atgggcaatg tcaattccat gcttgcgtgg gctcatgcca
    17941 gatgcttgga gaaccttctt gagacggtca gctgtgagct tgcagtgtcg gaccagttcg
    18001 gcgacgagtc atatcttaag aatgctttga tgcatcgagg gaggcagata actctggaac
    18061 agcggccacg ggccgaggag gatgttgcgg ttgcagccgc ctcgatactg gcccgagccg
    18121 agttcgttcg caggttagag tccctttctc aacaggccgg agttgagttg c
//
LOCUS       DAOSAI010000008        17228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_2289_length_17228_cov_3.933209, whole genome shotgun sequence.
ACCESSION   DAOSAI010000008 DAOSAI010000000
VERSION     DAOSAI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 17228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17228
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2289_length_17228_cov_3.933209"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..881)
                     /locus_tag="PLM83_00665"
     CDS             complement(99..881)
                     /locus_tag="PLM83_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK69957.1"
                     /translation="MEKSILVTAAVAALFSLLSIPGYAQDLPRGIDIARVTAERKPHV
                     LLVHGWLATSLDMSVLKYKLEQAGFECETINFSYLGAVSSIADYAVELARKVEVDCAG
                     KADVAIVAHSMGGLVTRYYLDNLRQTAQVSLVITIGTPHHGTLSGKIAPTASRCAREM
                     APGSELLRLLNAQEGDCPGVEFHAIWTPTDGVVIPAENAIMCGAKNYQVTGFGMAHMT
                     MLASDTVFEIVHAILRGQDVDVSGPQLRDHTEAEKPVVFPWQ"
     gene            957..2174
                     /locus_tag="PLM83_00670"
     CDS             957..2174
                     /locus_tag="PLM83_00670"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOK69958.1"
                     /translation="MDYAKRAQRLEPERAFSVLKKISEMVASGHDIIGFHIGEPDFTT
                     PENIKTAACSSIHANRTHYSPPSGVPAFQSAIADYISRTRGISVSPEQIVVAAGTKLA
                     IFCAIMSCVESGADVLGPVPSYPAYPSIVSMIGANFIPVPFIDTDQGFRLDLDRLASL
                     VTPNTSMIILNFPHNPTGTLLPREDLARIAEIAIEHDLWVLSDEIYSRLVYDGDFVSI
                     SSLPDMAERTILVDGFSKAYAMTGWRLGFAVVNTELAALFDTFMANAHSCTATFTQYA
                     GIEALTGPQHEVEKMRLKFLKRRDLVVTELNRVPGFRCHMPLGAFYAFPNVTEACRMA
                     GLADADQLQDALLDDAGVAVLSMSAFHSPNGPKDDQYIRISYAAGEREILEGTRRIRD
                     FMTSRIGRSGRHI"
     gene            complement(2182..3540)
                     /gene="dnaB"
                     /locus_tag="PLM83_00675"
     CDS             complement(2182..3540)
                     /gene="dnaB"
                     /locus_tag="PLM83_00675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOK69959.1"
                     /translation="MATADVVGVDRVPPQSLEAEQSTLGSMLLDKEAIATAAEILVAE
                     DFYRDAHRVIFNALISLFNKGEPADLITVTEALRQRNALENVGGASYISTLANTVPTA
                     ANCEYYAKIVKNKSIMRSLVTAGSQIAALGYDQTNEVAESLDKAEQLIFRISQRGETG
                     TVSDMKTVLMSTFDRIERLYTTKGAITGLSTGFAEVDNMLSGLQPSELIVIAARPSMG
                     KTALALNIAEHVGANEGKPVLIFSLEMSREQLAQRMLCSQATVDGQRLRRGNLSEADW
                     QRLSHAIGRLSEAPIFIDDSPTVTAMDIRARARRLKAEHGLSLIIVDYLQLVQGHARA
                     ENRNQEIAEITRSLKTLARELEVPVVSLAQLSRAVEATADKRPLLSHLRESGEIEQSA
                     DVVAFIYREDYYKPDTERRNIAEIIIAKQRNGPTGVVELVWQREYTRFRNLERFAQGE
                     SA"
     gene            complement(3547..5529)
                     /gene="lonC"
                     /locus_tag="PLM83_00680"
     CDS             complement(3547..5529)
                     /gene="lonC"
                     /locus_tag="PLM83_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon family ATP-dependent protease"
                     /protein_id="HOK69960.1"
                     /translation="MKSLVKKFTKIGSRNLADKLQDDEQLVRQVMALFEILSDIYGPD
                     RLVLKAGKLDALSLMRSAELDDRILALERIVFEDPTIDRKPSPEEYESVLADIEEEIA
                     DVIARRRVEDKIEKQISDRMQQRHEDYVNEIRLQILKEDSGPDTEDTLRKLAELEELE
                     KRRLAKSALEIVRPTAMSEIVGQDRAVRALLAKVASPFPQHVILYGPPGVGKTTAARL
                     ALEEAKKVALTPFGKESRFVEVDGTTLRWDPREVTNPLLGSVHDPIYQGAKRDLAEGG
                     VPEPKLGLVTEAHGGVLFIDEIGEMDPLLLNKLLKVMEDKRVKFESSYYDPSDPNVPS
                     YIRRLFEEGAPADFVLIGATTRDPSELSPALRSRTAEVFFEPLTQVDIENIVSNAAGK
                     LGVEMDPEVPSLVSDYTIEGRKATSLLVDAYGIALFREMDNRQDGKANEAASGDGPVR
                     ITLADLEETIRLGRLSPYVNVRASDSAEVGKIFGLGVAGFLGSVIEFEAVAFPAREEG
                     KGTIRFNETAGSMTKDSVFNAASVFRFLTDRDLADFDVHVNVVGGGDIDGPSAGTAVL
                     LAILSSVYACPIRQDVAVTGELSIQGKVRAVGGIFEKIYGARQAGIKKVILPAENVKD
                     VPPGITGIEVVSVSDVVDTFKHVFEGDAIFGELRRE"
     gene            complement(5583..6035)
                     /gene="rplI"
                     /locus_tag="PLM83_00685"
     CDS             complement(5583..6035)
                     /gene="rplI"
                     /locus_tag="PLM83_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOK69961.1"
                     /translation="MKVVLLKDVPGLGKMDTALDVAEGYARNYLIPRGLAIPATSKEL
                     NRVNKLREAEKRKEVRLEEKAREDARKLKAQGVTIRAKAGETGRLYGSITSKDVADAI
                     HESLGMEIDRRKIELADSIRELGTFTATVRLFPGISADVTINVIDEGK"
     gene            complement(6110..7066)
                     /locus_tag="PLM83_00690"
     CDS             complement(6110..7066)
                     /locus_tag="PLM83_00690"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HOK69962.1"
                     /translation="MSQSRNQNLRALTEGALLAALTVVLYVADVYTRILIYVVPVPIA
                     ILVVKHDLRTGLMAAAVSALGVGLILGLIDSVIVFVRVATLGLTLGVCLAKLLPWPRT
                     IAITSVASTVQIAIDFMLAAVVSKLSPTEFLGSMMDGFAAAGERAVEIYEGMGMGETS
                     MMLARQSVELMTVLLKSFLPVIVLSAAVLGAVIVAAVARWVLVRMNEKVVPVPPFARW
                     QLGWEWIWGLIAGILLSQLGQWMGIELISTIGKNVTMGFALLYTVQGVAIIWHFFTKR
                     NIPKALAVIAIVLIYLTPPFNLLVPIAGVLDTWLDFRNLAAQ"
     gene            complement(7063..7377)
                     /locus_tag="PLM83_00695"
     CDS             complement(7063..7377)
                     /locus_tag="PLM83_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HOK69963.1"
                     /translation="MPYPRYTDIACGVKTIDWLEAEILGSIASLYRSLARADEGPRVA
                     DLANAIIGCYLLGKRLGISYAELEQQISANIDENIEKGHEIERWYGDFSALANHRKGR
                     ST"
     gene            complement(7573..7803)
                     /gene="rpsR"
                     /locus_tag="PLM83_00700"
     CDS             complement(7573..7803)
                     /gene="rpsR"
                     /locus_tag="PLM83_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOK69964.1"
                     /translation="MRRERGRKSRRRVCSFCVDKVQDIDYKDTARLRRYLNERGKIIP
                     RRISGNCAKHQRQVTVAIKRARQMALLPYTAD"
     gene            complement(7844..8299)
                     /gene="ssb"
                     /locus_tag="PLM83_00705"
     CDS             complement(7844..8299)
                     /gene="ssb"
                     /locus_tag="PLM83_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOK69965.1"
                     /translation="MMNRVVIVGRLTHDPELRFTQNGIAVTRFSLAVDRPFTNQQGER
                     ETDFLDVVVWRRQAENVAAYLKKGSLAAVDGRVQVRSYETPEGQKRKVWEIVADRVEF
                     LDRRGDTGSQMSQGGYGRTQPELGEDEDDVPMPEEAETFGDQIDPDIRL"
     gene            complement(8339..8626)
                     /gene="rpsF"
                     /locus_tag="PLM83_00710"
     CDS             complement(8339..8626)
                     /gene="rpsF"
                     /locus_tag="PLM83_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOK69966.1"
                     /translation="MRAYESMFVIAPTVPEEEIDAFIDRFVQLIVNNGGEVTEIDKMG
                     RRRLAYEIEDFTEGYYVVLYFNAQPDAVAELERVYKITDNVIRSMVVRKDS"
     gene            complement(8764..10014)
                     /gene="ychF"
                     /locus_tag="PLM83_00715"
     CDS             complement(8764..10014)
                     /gene="ychF"
                     /locus_tag="PLM83_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOK69967.1"
                     /translation="MLNLGIVGLPNVGKSTLFNALTRGRAQVANYPFTTVDPNMGMVQ
                     VPDNRLDAVAAVAGAQKVTPAAIRFVDIAGLVRGASRGEGLGNKFLSHIREVDAVVHV
                     VRCFFDTDVAHVYNRPDPVRDVEIIETELILADLESVDKRIAKTEPLLKTGDKRCQFE
                     MEVLTSLKKHLESGLPARQAQLDAEEREVVDELFLLTMKPVILVANLAEECAEQLGEL
                     LPMNSISGSDASRVVPNDVASLHALEDGASQDGDGGAKGTGEGSNSGIIGEIESILDA
                     LSSVAHGAPVIPVCAELERQLSELPPDDASDYLASLGLAHSALDSLIATGYSILDLIT
                     FFTIKGPETRAWTVARGTVAPKAAGKIHSDMERGFIKAEVIQWDELVGEGSFVAAREH
                     GKVRVEGKDYVMQDGDVVLFRFNV"
     gene            complement(9983..10210)
                     /locus_tag="PLM83_00720"
     CDS             complement(9983..10210)
                     /locus_tag="PLM83_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOK69968.1"
                     /translation="MSYRLGDIVRMKKPHPCGSSTWEILRVGADFRIKCTGCGRVVML
                     TRAQFMKDVRGIADSGAGGDVHAESGNRGPA"
     gene            complement(10207..11142)
                     /locus_tag="PLM83_00725"
     CDS             complement(10207..11142)
                     /locus_tag="PLM83_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOK69969.1"
                     /translation="METEALSSSAVDDIVAGGFSKQALTDKTVVILSSLIKIGTIVLI
                     SALILRISFRVIDRLFRSREDEHIALFKDTRRLVTLRTVSKSIVRYVVYFIGGIMILS
                     ELGVDTASLIAGAGIVGLAVGIGAQNLVKDVITGFFVLFEDQYAVGDYIAVAGVSGVV
                     ESIGIRVTRIRDFAGQLYIIPNGSIGMVTNYRGHNMRVLFDVIVPYSTDIEHALRVLQ
                     KAFDEQKASIPGIADGPRVLGVQEMNETGVFLRVWATAKPMEQWSVERQLKMIARKAL
                     DDAGVDPPVTHQRIIIDRFSDPVGSQAEEGGEYEA"
     gene            complement(11278..11613)
                     /locus_tag="PLM83_00730"
     CDS             complement(11278..11613)
                     /locus_tag="PLM83_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOK69970.1"
                     /translation="MSQWLGHIVRLVVSAIVLMLISSLIPGFASLTFGTALVAAVVIT
                     VLGWLVEAILGHGVSPYARGIVGFLVSAVVIYATSFIVPGMTITVAGALLAAFVIGLV
                     DMFVPTTLR"
     gene            complement(11757..12764)
                     /locus_tag="PLM83_00735"
     CDS             complement(11757..12764)
                     /locus_tag="PLM83_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HOK69971.1"
                     /translation="MPEFRQDIITGNWVIVAKERAKRPEDFSEPEKPRKERFRENCPF
                     CVGNEKQTPPEVYAIREEGAEPDTPGWKVRAVPNKFPALTSNGQVNTGDVGIYRTMSG
                     IGAHEVILETPDHDRSPAYLTEEEIADVIATYRHRHRVLLEDERIQYVSIFRNHGRIA
                     GASLEHPHSQIIATPIVPPLVQHSISSAKDYHDRTGRCVYCHVLEEERKAGVRVVYSN
                     PGFIVFEPFASRTPFETWIMPLRHSPSFGDISDQEIEQLADALGLTLRKIAQGLNDPA
                     YNYMIRTAPTVSEYDGAEYLHWHISILPKLAIAAGFEMGTGIYINTATPEDTAKFLRS
                     I"
     gene            complement(12783..14285)
                     /locus_tag="PLM83_00740"
     CDS             complement(12783..14285)
                     /locus_tag="PLM83_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOK69972.1"
                     /translation="MRGGKGGTFPEIPEMHRQISMIVSLATFEHLPKRVGGLAEAATS
                     IGESLARENEVMVFMPSHGIHKTEKTLKIRKYGKFALDAGSASTQITMYEMERNGVRV
                     FLLSDEVMDHPDVYMPREMLTAKILRFAYAFPAAINLIMGKEGKKPDVAHINDWHCVL
                     GGAAAKKYLEIPMAYTIHRICREKIAVKDMEDAGLSEFIDPESIEISGKREMFNLETY
                     GCRVCDHLNTVSYSYLCEEWDEFFGKYAGKATYVWNGMDHSFWDPAKLERAGMPRRER
                     RAALLKENGMEDGTLYFYVGRFDMEQKGVDHMFEAIRMIFDGEVAGADRLIPNLRVVV
                     LGSGDPVLEAMARDMEKKYPKNVRAIIGYLGRETTREYYGASDFCLIPSNFEPFGLVQ
                     LEAMCMGSIPIGTRVGGINDTVLDIDKAKENATGKLVSPRKPKELAAAMVKMGILDMD
                     DPSKIEKIRANGRPHVMTNFTWDRAAQRYLAMYQGKASIMLPFADYGGPF"
     gene            complement(14594..15115)
                     /locus_tag="PLM83_00745"
     CDS             complement(14594..15115)
                     /locus_tag="PLM83_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOK69973.1"
                     /translation="MNLLHIYLSFAKVGLFTFGGGYAMLPLIESDIIQKRGWLTMAEF
                     TDMVAIAEVTPGPIAVNSATFVGYKLAGIIGGIVATLGVITPSIVIVLALAALVRKYE
                     RTATLSRIKVGMRPAVAGLIAAAAISVAKVSMAEPRSYAVAAAAAVMVFVGKIDPILV
                     LAVCGILGALIFS"
     gene            complement(15112..15723)
                     /locus_tag="PLM83_00750"
     CDS             complement(15112..15723)
                     /locus_tag="PLM83_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOK69974.1"
                     /translation="MDSRQVSGMTCCEKETDNGDGGSKSASLWDMFWTFFRIGAFTIG
                     GGYVMVPLIQKDVVDRKGWVTKDEFIDALAIAQTAPGPIAVNTSVYVGYALRGFAGAA
                     ASVAGCIIPSIIIILMIASVFDRISSLDVVQAIFSGIRPAVAVLIASAAIKLGKPVIR
                     SAGELAIAAAAAIMVSLLHISPALVVVIAALAGLLTGIGGDSR"
     gene            complement(15696..16193)
                     /locus_tag="PLM83_00755"
     CDS             complement(15696..16193)
                     /locus_tag="PLM83_00755"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOK69975.1"
                     /translation="MTRVLLAVEIASFILMGFVIGSYVDSKQETRPWGTIIGFLVGLF
                     LGVMSVRRAIDEISSRAVAGEGPKVVKVVRRPISVRADEASGDPKAFYYRGTEHKVEA
                     VMSKWRDFTSFDKAMLGGKRKERDWWLNGCGKVNYRIRTEDGRVFDLQYDERKSEWIL
                     DKFLE"
     gene            complement(16224..17105)
                     /locus_tag="PLM83_00760"
     CDS             complement(16224..17105)
                     /locus_tag="PLM83_00760"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOK69976.1"
                     /translation="MDTVEFINALRKATTSRSPRELEIPVRRMPAKCSTASIEAKGIR
                     RLISAYTTKFDARKTNRVNNIRLGAQKLNNRMLAPGEVMSFNDVVGPRIPERGFMEAD
                     IIFDSQLVPGVGGGICQVSTTLYNAALLGLMEPVSRTNHSLPISYVSMGRDAAVSYGS
                     IDLKIKNTTAHYVLIRSYVGSDTVSFKIYGDMPDNMDVSIYTEVVERLEPSTIERVDY
                     LAPPGSVRVESDGKPGYVVSVWRIIKVDGVEARRELISRDRYKPQPKVVVLGPTAAPT
                     ATEPEPSLPLIVDPPQG"
BASE COUNT         3977 a         5194 c         3974 g         4083 t
ORIGIN      
        1 tatcatgcaa cacttccgaa ctcctcacag tctacggcat gcggaacttc tgccgagaat
       61 agcctggacg ccgccacagg gtctggcaca atttcatgct actgccatgg aaaaaccaca
      121 ggcttttcag cctctgtatg atctctcagc tgaggaccgg atacgtccac atcctgcccc
      181 cgcagtatgg catggacaat ctcaaagacc gtatcgctcg caagcatcgt catatgagcc
      241 attccaaagc ccgtgacctg atagttcttc gcgccgcaca ttatagcatt ctccgccgga
      301 ataaccaccc catccgtagg agtccagatc gcatggaatt caacacccgg gcaatccccc
      361 tcttgagcat tgaggagccg caaaagctca cttcccggag ccatctccct agcgcatcta
      421 cttgcggtgg gagcgatctt gccactcagt gtcccatggt gcggagttcc gatggtgatg
      481 accagactca cctgggccgt ctgcctcagg ttatccaagt agtatctggt aaccaatccg
      541 cccatgctat gagccacgat tgccacatca gccttgcccg cacaatcgac ttccaccttt
      601 ctcgccagct ccacggcgta gtcggctatg gagctcaccg ccccaagata cgagaaattg
      661 atggtttcgc attcaaaccc ggcctgttcc agcttgtatt tgagcacgct catgtccagg
      721 gacgtagcca gccagccatg gaccaacaga acatgaggtt tcctctcagc agtcacccgg
      781 gcaatatcga tgcctcgcgg gagatcctgc gcatacccgg gaatactgag taaagagaaa
      841 agcgcagcca ctgcagcagt gaccagaata ctcttctcca tgcgaataac cctccagtcc
      901 acattgtgtg cagggcgctt cgcgccacaa tctctgtagt tggagatgat gaatgaatgg
      961 actacgcaaa aagggctcag cggctcgaac ccgagcgcgc cttttcggta ctgaaaaaga
     1021 tttctgaaat ggttgcctca ggtcacgaca taataggttt tcacataggt gaacccgatt
     1081 tcaccacgcc tgaaaacata aaaacggcag cttgctcttc aatccatgct aacaggacac
     1141 attactcgcc cccgtccgga gtgccggcct tccagagtgc catcgccgac tatatttcca
     1201 gaacccgggg aatatccgta tctcccgagc aaatagtcgt ggccgcaggg accaaacttg
     1261 cgatattctg cgcgatcatg tcctgcgtag aaagtggcgc cgacgtcttg ggacctgtcc
     1321 cctcataccc ggcctacccg tcaatagtaa gcatgatcgg agcaaacttt attccagtcc
     1381 cattcatcga taccgaccag ggctttcgct tggacctcga tcgccttgcg agcctggtaa
     1441 ccccgaatac ttccatgata atcctgaact tcccgcacaa cccgaccggc acactcctcc
     1501 cacgggagga tcttgcgcgc attgccgaaa tcgccatcga acacgacctg tgggtcctgt
     1561 cagatgagat atactcccga cttgtctacg atggcgactt cgtcagcatc tcgtccctgc
     1621 cagacatggc agagcggaca atcctcgttg atggtttctc aaaagcctat gccatgaccg
     1681 gctggcgtct gggtttcgcc gtcgtcaaca cggagcttgc agccctgttc gacaccttca
     1741 tggccaatgc gcactcctgc acagccacat tcacccaata cgccggaata gaggccctca
     1801 cagggcccca acatgaagtg gagaagatgc gcctcaaatt cctgaagcgg cgggacctcg
     1861 tcgtgacgga gctcaaccgt gtaccaggtt tcagatgtca catgccccta ggggcattct
     1921 atgcctttcc caatgtcaca gaagcgtgcc gtatggctgg tcttgctgac gcagatcagc
     1981 tccaggatgc cctcctggac gacgcaggcg tagcagtcct gtccatgagt gcctttcatt
     2041 cgccaaatgg ccccaaggac gaccagtaca tccggatatc ctatgccgca ggcgagaggg
     2101 aaatcctcga gggcacgcga cggattcggg acttcatgac ctcccgaatc ggcagatcgg
     2161 gtcggcatat ctgaccacgg cttatgccga ctcgccctgg gcaaacctct ccagattccg
     2221 aaaccgcgtg tattccctct gccaaaccag ctcaacaaca ccggtcggtc cgttgcgctg
     2281 cttagcgatg attatctccg cgatattcct ccgttccgtg tcgggcttgt agtaatcttc
     2341 acggtagatg aacgcaacca cgtctgccga ttgctcgatt tcaccgctct ctctgaggtg
     2401 tgacaatagc gggcgcttgt cggcagtcgc ttcaacagcc ctgctgagct gcgcgaggga
     2461 aaccactggc acctccagct cgcgtgcgag cgtcttcagt gatcgggtaa tctctgcaat
     2521 ctcctggttc cggttctccg cccgcgcgtg gccctgaacc agctgtagat agtcgacgat
     2581 gatcaacgac aggccgtgct cagcctttaa ccgtctcgcc ctggcacgta tgtccattgc
     2641 tgtaaccgtc gggctatcat cgatgaatat aggggcctcg ctgagccggc cgatcgcatg
     2701 cgacagacgt tgccaatccg cttccgacag attgcctcgt ctgagtcgct gaccatccac
     2761 cgtcgcctgg gagcagagca ttctctgggc gagctgctcc ctagacatct cgagactgaa
     2821 aatgaggaca ggctttcctt cattggcccc aacatgttcg gctatgttca gcgctagcgc
     2881 cgtctttccc atagacggtc gcgctgcgat gacaatcagt tcggacggct gcagccccga
     2941 cagcatattg tccacctcgg caaatcccgt ggaaaggccg gtgattgccc ctttcgtggt
     3001 gtaaaggcgc tcgatccgat caaaagtgct catcagcacc gtcttcatgt cagagaccgt
     3061 gcccgtctcc ccacgttgcg agatcctgaa tatgagctgc tcagccttgt ccagcgactc
     3121 cgctacctcg ttcgtctggt cgtagcccaa agcagctatc tgcgatcctg ctgtaaccag
     3181 agatcgcata atggacttat tcttgacgat cttggcatag tattcacagt ttgcagccgt
     3241 cgggaccgtg tttgcaagcg tgctgatgta cgaagccccg cccacgttct ccaaagcatt
     3301 gcgctggcgc aacgcctctg taacagtgat taagtcagcc ggttcaccct tgttgaacag
     3361 ggagataagg gcattgaata tcactctgtg cgcgtctctg tagaaatcct cagccacaag
     3421 gatttcggca gctgtagcaa tcgcctcttt atcgagcaac attgaaccta atgtcgattg
     3481 ctccgcctcg aggctctgcg gtggcacacg atcaacgccc actacatccg cagttgccaa
     3541 agtaactcac tccctacgca attcgccaaa gattgcgtcc ccctcaaaaa catgcttgaa
     3601 cgtgtcaacg acatctgaaa ccgacaccac ctcgataccg gtaatgccgg gagggacatc
     3661 tttgacattc tcggccggca agatcacttt cttgatgccc gcctgtcgcg ccccgtatat
     3721 cttctcgaag attcccccca ccgcccgtac cttcccctga atggaaagct cgccggtaac
     3781 tgcaacatcc tgcctgatcg ggcatgcata aacagaacta agaattgcaa gaaggaccgc
     3841 tgtcccggca gaaggcccat cgatatcgcc cccgcctaca acattcacat ggacatcgaa
     3901 atcggcaagg tccctatcag tcagaaatct gaatacagaa gctgcattga atacagaatc
     3961 cttcgtcatg ctacctgccg tctcgttgaa tctgatggtc cccttgccct cctcgcgagc
     4021 cgggaacgca acggcctcga actctatcac tgagcccaag aatccggcca ccccgagtcc
     4081 aaagatcttc cccacctcgg cgctatcaga ggcgcgaaca ttaacgtacg gcgaaagccg
     4141 tccgagacgg atcgtctcct caagatcggc caatgttatc ctgacaggac cgtccccgga
     4201 tgcagcttcg ttcgccttcc cgtcctgcct attatccatc tctctgaaca aggcgatgcc
     4261 ataggcgtcc accaaaaggc tcgtggcctt tcttccctcg atggtatagt cgctcacaag
     4321 agacggaacc tcaggatcca tctccacccc tagcttccca gcggcattgc tcactatatt
     4381 ctctatgtcc acctgggtaa gtggctcaaa gaagacctcc gcagtccgtg aacgcagagc
     4441 cgggctgagc tctgagggat cacgagttgt ggccccgatc agcacgaagt ctgccggcgc
     4501 gccctcttca aaaagccttc tgatatatga cggcacattc gggtcagaag gatcgtagta
     4561 tgaagactcg aacttgaccc tcttgtcctc catgaccttg agcaacttgt tcaagagcaa
     4621 tgggtccatt tccccaattt cgtctatgaa caacaccccg ccgtgggcct ccgtaacgag
     4681 accgagcttg ggctctggaa ctcccccttc agccaggtcg cgctttgcgc cctggtatat
     4741 cgggtcatgc actgaaccga gaagcgggtt cgtgacctcc ctgggatccc atctgagggt
     4801 cgtgccgtcc acttccacga acctcgattc cttgccaaac ggcgtaaggg caaccttctt
     4861 ggcctcctca agggcgaggc gcgcagcggt cgtcttccct actcctggag ggccatacaa
     4921 gattacatgc tgtggaaatg gcgaagccac cttggcaaga agggctctga ccgcacgatc
     4981 ttgccccaca atctccgaca tggcagtcgg acgcactatt tccagcgcgg acttggcaag
     5041 tcgcctcttt tcgagctctt ccagttcagc caacttcctg agggtatctt cagtatcggg
     5101 tccggagtct tccttcagta tctgcaatct tatctcattt acgtaatcct catgcctctg
     5161 ctgcatgcgg tcggatattt gcttttcaat cttgtcctct acccgccgcc tcgctatcac
     5221 atccgctatc tcttcctcaa tatcggccaa gaccgattcg tactcttcag gcgatggctt
     5281 gcggtcaatg gtgggatcct caaatactat tcgttccaga gccaaaatcc tgtcgtcaag
     5341 ttccgcagaa cgcatgaggc tcagcgcatc aagctttcca gccttcagaa ctagcctgtc
     5401 aggcccataa atatctgaaa ggatctcgaa aagggccatc acctgccgca caagttgctc
     5461 gtcgtcctgg agcttatccg ccaggttcct gctgccgatc ttggtgaatt tcttaacgag
     5521 cgacttcatg aaagcaaaac ccctttcgtc tctgccctaa ggtacgcctt gggcaactat
     5581 cttcattttc cttcatcgat gacattgatc gtaacatcgg cgcttatccc gggaaaaagc
     5641 ctcacagttg cagtgaacgt gccaagttcc ctgatggaat cggccagctc gatctttctg
     5701 cgatcaatct ccatccccaa cgactcatgg attgcatcgg caacgtcttt tgacgtaatt
     5761 gagccgtaaa gtcttccagt ctcgccagcc ttagcccgaa tagtcacacc ttgtgccttc
     5821 aatttgcgcg catcctctct ggccttctcc tcaaggcgaa cctccttgcg cttttcagcc
     5881 tcgcgaagct tgtttactct gttaagctcc ttggaggttg ccggaatagc caggccccgg
     5941 ggaatcaggt agtttcgggc atatccttca gccacatcta gcgcagtgtc catctttccc
     6001 aagcccggga catcttttag aaggactact ttcacgactt ttcgcgggcg aagcgtatta
     6061 tttcgttccc cgcccgctac cccctttcaa agtacgttgg cgcacagccc tactgcgccg
     6121 ccaagtttct aaaatcaagc catgtatcga ggactccggc tatggggaca agaagattga
     6181 acggcggagt caggtatata aggactatcg cgataacggc cagggctttc ggaatatttc
     6241 gcttcgtaaa gaagtgccag ataatcgcga ctccctgtac agtatagagc aaggcgaacc
     6301 ccatagtcac gttcttgcct atagtcgata tgagctcaat gcccatccat tgcccgagtt
     6361 gcgaaaggag aatccccgct atcaagcccc atatccactc ccacccaagc tgccatcggg
     6421 caaagggcgg gacgggcacc actttttcat tcatgcgcac aagcacccag cgagcaacgg
     6481 ctgccactat caccgcaccc aacactgcgg ctgacagcac aatcacaggc agaaacgact
     6541 taagcaagac ggtcatgagc tcaacggact gcctggcgag catcatggat gtttcgccca
     6601 ttcccatacc ttcatagatt tcaacagctc tttcgccagc cgccgcgaat ccatccatca
     6661 tgctgcccag gaactctgtc ggagagagtt tggatactac cgcggccaac ataaaatcga
     6721 tggcaatctg cacggtcgaa gcgacactgg taatggcgat agtgcgaggc catggaagca
     6781 attttgccaa gcatactccg agggtcaaac cgagagttgc gacacgcaca aagacgatca
     6841 cagagtctat tagccccaaa ataagcccta caccgagagc cgagaccgct gcggccatga
     6901 ggcctgtgcg gagatcatgc tttactacga gaatcgcaat gggcactgga accacgtaaa
     6961 tgagtattct ggtataaacg tcagccacat acaacactac tgtaagtgcg gcaagaagag
     7021 ccccctcagt aagcgctcgc aagttttgat tccgtgattg actcaagtgg aacgcccctt
     7081 cctgtgattc gccaaagcgc taaagtcccc ataccacctt tcgatttcgt gccccttctc
     7141 gatgttctca tcaatgttcg ctgagatttg ctgttccagc tcagcataac ttattcctaa
     7201 ccttttgcct agcagatagc aaccgatgat ggcgttagcc agatcggcca cgcgcgggcc
     7261 ttcatccgcg cgcgccagag aacgatacaa cgaggcaatg cttccaagta tctccgcctc
     7321 aagccagtca attgtcttaa caccacaggc aatatcggtg tatctcggat atggcattcc
     7381 gactcctccc cgtgttaaag tgccatgcag tcaatggact cgttcgttct cgacaccaaa
     7441 ggcaaaatcc ttccgcccaa ccttccaatg caatgtagga cacatgggcg tatgtgcaag
     7501 aaaactcctt gggggctgct gccgccaaag agttttccaa tccgttatag ccacggttcc
     7561 atctaaacca gactagtctg cagtatacgg cagcaacgcc atctgccttg cccgctttat
     7621 ggcgaccgta acttggcgct gatgcttagc gcagttccca gagatccggc gcggaatgat
     7681 cttcccgcgc tcgttgaggt atctccgaag cctggctgta tccttgtaat ctatgtcctg
     7741 aactttgtcg acgcagaagg aacatactcg ccttctggac ttgcgtcctc tttcacgtct
     7801 cacctatatt gtccctccac tgatgtaggc agtggtgctg tcgctataag cgtatgtccg
     7861 gatctatctg atccccgaat gtctcagcct cttcaggcat tggaacatcg tcttcatctt
     7921 cacccagctc cggctgtgta cggccatacc caccctggga catctgtgat cccgtatcgc
     7981 cccttcggtc gagaaactca accctatcag cgactatttc ccataccttc ctcttctggc
     8041 cctcgggcgt ttcatagctg cgaacctgta cacggccatc cacggcagca aggctgccct
     8101 ttttcaggta tgccgctaca ttctcagctt gcctgcgcca gactaccaca tcaaggaaat
     8161 ctgtttcacg ttctccctgc tggtttgtaa aaggcctgtc aacagccaaa gaaaatctag
     8221 tgacagctat gccattctga gtgaatcgaa gttcgggatc atgggtcaag cgccccacta
     8281 ttacgaccct gttcatgatt acaccaccct gccttgatat ttgcagcccg gcaaggaatt
     8341 agctgtcctt tcggacgacc atagagcgta tcacgttgtc tgtgatcttg tacacccgct
     8401 caagctccgc tacagcatca ggctgagcat tgaaatagag caccacgtag taaccctccg
     8461 tgaaatcctc tatttcgtac gccagccggc gcctgcccat tttgtcgatc tcagtcactt
     8521 cgcctccatt gttcacgatt agttggacga acctgtctat gaaggcatcg atctcttcct
     8581 cgggcactgt aggagcaata acaaacatgc tctcgtaggc gcgcactgac ttcacctccc
     8641 ctcggactat cggccctacg ttccacgcag ggcagggatt gcgacatgaa ttataacatc
     8701 cacatcagac aggcgcaacc cactatgcgg gtgaaaaatc ccaagtatcc gggctcaccc
     8761 tgattataca ttgaaacgaa aaagcactac gtctccatcc tgcattacat agtctttccc
     8821 ctctactctt accttgccat gctctcgagc tgcaacaaaa gatccttctc caacaagctc
     8881 atcccattgt atcacttccg cttttatgaa cccgcgctcc atgtctgaat gtatctttcc
     8941 cgcagctttt ggcgccactg taccccgagc gacagtccat gcccgagttt cagggccctt
     9001 aatcgtaaag aaggtgataa ggtcaagtat gctgtaccca gtggcaatca gactgtccag
     9061 cgcggaatgg gctaacccta gggatgccag ataatcgctg gcatcatccg gaggtagctc
     9121 agagagctgc ctttctagct ctgcacatac cgggataact ggcgccccgt gggcaaccga
     9181 actcaacgca tcaagaatgg actcgatctc cccgataatc cctgagttcg agccctctcc
     9241 agtccccttt gcgccgccgt ccccatcctg gctcgcccca tcttcgagag catggagact
     9301 tgccacatca ttgggaacaa ctcgactggc atccgagcct gagatcgaat tcatcggcaa
     9361 aagctctccc agctgctccg cacattcctc cgctaaattc gccactaata tcacaggctt
     9421 catagtaagc aagaagagtt cgtccacgac ctcgcgttcc tcggcgtcca gttgcgcctg
     9481 gcgtgccggc aatccagatt cgagatgctt cttgagactg gtcagaacct ccatctcgaa
     9541 ttggcaccgc ttatcccctg ttttcaaaag cggttcggtc ttggcgatgc gcttgtccac
     9601 gctttccagg tctgccagta tgagctctgt ctctattatc tcgacatccc tcacaggatc
     9661 cgggcgattg tagacgtgcg ctacatcggt atcaaagaag cacctcacca cgtgcactac
     9721 agcatctact tccctgatat gggacagaaa cttgtttccg aggccttcgc ctctacttgc
     9781 cccgcgaacc agcccggcta tgtccacaaa tcggatcgcg gcaggagtca ctttctgcgc
     9841 ccccgcaacg gccgcaacgg catcaagccg attgtcaggg acctgtacca ttcccatgtt
     9901 gggatcgaca gtagtgaacg ggtagttagc tacctgggct cgcccgcggg ttaacgcgtt
     9961 aaacagagtg gacttgccta cattaggcag gcccacgatt cccagattca gcatggacat
    10021 cgcctcccgc gccagaatca gctatccccc gcacatcctt cataaactga gctctcgtaa
    10081 gcataaccac gcggccacaa cccgtgcact tgatgcggaa gtccgcgcca accctgagaa
    10141 tctcccacgt tgagctgccg cagggatgag gtttcttcat tctcactata tcgcccagcc
    10201 gatagctcat gcttcgtatt ccccgccttc ctcagcctgt gagcctactg gatccgaaaa
    10261 tctatcaatg attatgcgtt ggtgcgtcac tgggggatct actccggcat catcaagagc
    10321 ctttctcgct atcatcttca gctgcctctc tacagaccac tgctccatgg gcttcgcagt
    10381 tgcccacact cggaggaaaa cccccgtttc attcatctct tggactccca atacgcgggg
    10441 accatccgca atccccggaa tgctcgcctt ctgttcgtcg aaggccttct gaagcacgcg
    10501 cagtgcatgt tctatgtcgg tagaatacgg gactatcaca tcaaacaaga cccgcatgtt
    10561 atgcccacga taatttgtta ccatgcctat agaaccattg ggaataatgt agagctgccc
    10621 ggcaaagtcg cgaatgcgcg tcacccgtat tccaatcgac tccaccacgc ctgagacccc
    10681 ggcaaccgca atgtaatctc cgacggcata ttggtcctca aatagaacga aaaatcccgt
    10741 tattacatcc ttgacaaggt tctgagcgcc aattcccaca gccaatccca caattcctgc
    10801 tcctgcaatc aggctggcag tatcgactcc tagctcagaa aggatcatga ttcctccgat
    10861 gaaatataca acatacctga caatgctctt gctcaccgtt ctaagagtaa caagacggcg
    10921 cgtgtccttg aacaaggcga tatgttcatc ttcgcgactc ctgaaaaggc gatcgatcac
    10981 gcgaaaggaa atgcgaagaa tcagggcaga gatcaagact atcgtcccta tcttgatcaa
    11041 ggacgatagg ataaccaccg ttttatccgt aagagcctgt ttggagaatc ctccggccac
    11101 aatgtcgtcc actgcggagg atgacagcgc ttctgtctcc ataagcgacc acccctatct
    11161 tcacacatag cttatgttag acatcacctc gacttgtcct gcctgataaa gtgaaacccc
    11221 tcccggccaa ctgccgaaag gggtgggcgc aggcatcaat tgtttcacgt gaaactacta
    11281 gcgcaaggtg gtcgggacaa acatgtcaac aagcccaatc acaaatgccg ctagcaaggc
    11341 gcctgcaacg gttatggtca tgcccggcac tatgaacgac gtagcatata tcaccactgc
    11401 tgacacgaga aagcccacaa taccccttgc atacggggat accccatgcc cgaggatggc
    11461 ttcgaccagc catccaagca cagttatgac caccgctgca actaatgccg tcccaaacgt
    11521 taaacttgca aaacctggta tgagagatga gattagcatc aacacgatcg ccgacacaac
    11581 aagtctcaca atatgtccta accactgact catcgactct tctgcccccc cagtacatgt
    11641 gctggtttga ttcgccaatc gtggttatag gataaccagc attgggggga ctatgctcga
    11701 tcttgctccg cgagcgcgaa agcgctcgca cctgggccga aaaaacagac ctcccgtcat
    11761 atactcctca ggaatttagc tgtgtcctcg ggtgtcgcag tattgatata tatgcctgtc
    11821 cccatttcga acccggccgc tatggccagc ttaggcaata tgctgatatg ccaatgcaag
    11881 tactccgcgc catcgtattc gctaacggtc ggagcagtgc gtatcatgta attgtatgcc
    11941 ggatcgttca gtccttgggc gatcttacgc agggtcaatc cgagagcatc tgcaagctgc
    12001 tcaatctcct ggtctgagat gtcgccgaag gatggggaat ggcgcagcgg cattatccat
    12061 gtctcaaacg gcgtcctcga cgcaaatggc tcaaacacga tgaacccagg gttgctgtat
    12121 accactcgca cgcctgcctt ccgctcctcc tcaaggacat ggcagtagac acatctgccc
    12181 gtgcgatcgt ggtaatcctt ggccgatgag atagaatgct ggacaagagg cggaactatt
    12241 ggcgtagcta ttatctgcga atgcggatgc tccagagaag ctcctgcaat tctgccatgg
    12301 ttgcggaaaa tagacacata ttggatcctc tcatcctcca agagcacgcg gtggcgatgg
    12361 cgataggttg ctattacatc tgcgatctct tcctcagtca gataggcagg acttctgtca
    12421 tgatcaggcg tctccaaaat tacctcatgg gcccctatgc ctgacatcgt gcgataaatg
    12481 ccaacatcgc ccgtgttaac ctggccattt gatgtaagcg cgggaaactt gttggggacg
    12541 gccctcactt tccagccagg ggtgtcgggt tctgcgcctt cttccctgat ggcatagacc
    12601 tcaggtggcg tttgcttctc gtttcctacg cagaatggac agttttcgcg aaaacgttcc
    12661 ttacggggtt tctccggctc ggaaaagtct tccggccttt tagccctttc cttcgcaacg
    12721 ataacccagt ttcctgtgat gatgtcttgc ctgaattctg gcatggtgcc tggcctcctt
    12781 gattaaaacg gacctccgta atccgcaaat ggcaacatga tcgatgcctt tccctggtac
    12841 attgccaaat atctttgggc cgcccgatcc catgtgaaat tggtcatgac atggggcctg
    12901 ccattggccc tgatcttctc gatcttagaa gggtcatcca tgtcaagaat gcccattttg
    12961 accatggcag cagctagttc cttgggttta cgtggggaaa ccagcttgcc ggtggcgttt
    13021 tccttggctt tgtcgatgtc aaggactgta tcgtttatgc cccctactct tgtgcctatg
    13081 ggaatcgacc ccatgcacat cgcctcgagc tgaacaaggc caaagggctc gaaattggac
    13141 ggaatcagac aaaagtctga tgctccatag tattccctag tagtttccct tcctaagtat
    13201 cctatgatcg ctcttacgtt tttcggatac ttcttctcca tgtcccgggc catggcttcg
    13261 agtactggat ctcccgagcc taggaccaca accctcaagt tcggaattag cctatctgcc
    13321 ccagcaacct ctccatcgaa gatcattcgg attgcctcaa acatgtggtc tacgcctttt
    13381 tgctccatat cgaaccggcc aacataaaag tagagcgttc cgtcttccat tccattttct
    13441 ttgagcaacg cagcgcggcg ttccctcctt ggcatgccgg cccgttcgag tttggccgga
    13501 tcccaaaagg aatgatccat cccattccaa acgtaggtcg cctttccggc atacttcccg
    13561 aaaaactcat cccattcctc acaaaggtat gagtagctga cggtatttag atggtcacag
    13621 accctacagc catacgtttc gaggttgaac atttcccgct ttcccgagat ttcgattgat
    13681 tcagggtcga tgaattccga taggcccgcg tcctccatat ccttcacggc gattttttcg
    13741 cggcagatgc ggtgtatggt gtaggccatg ggtatctcga ggtatttttt ggctgcagct
    13801 cctcccaaca cgcaatgcca atcattgatg tgggccacat ccggtttctt cccttccttc
    13861 cccatgatca agttgattgc ggcaggaaag gcataagcga atcgaagtat cttggccgtc
    13921 agcatctccc ttggcatgta tacatctggg tggtccataa cctcatcact caacaagaaa
    13981 acgcgtactc catttcgttc catttcatac atggtaatct gcgttgaagc tgaacctgcg
    14041 tccagagcaa acttcccgta ctttctgatt ttcagcgtct tctcggtctt atgtatgccg
    14101 tgtgacggca tgaagaccat tacctcgttc tccctggcta gagactcccc tatagacgtg
    14161 gcagcttctg caagaccccc aacacgtttg ggcaggtgtt cgaaggtagc aaggctaaca
    14221 atcatggata tctggcggtg catttccggg atttccggaa atgtgccgcc cttccctcct
    14281 ctcatatgag tttggcacgc ttttgcgatc ctcgcggcta taacctagcc gccccttcaa
    14341 gccgcttcca gacgctcgct ctctgccagg ccgtgtaaag cggcatggaa tcatcctaag
    14401 cacttcacaa actgctgact tgctttaata tgccccatgc catcactttc ggctgcacga
    14461 aaaatataat gaaatgcagc cgtcacgcgg ccctggaatc atcgggaggg atgcgatatg
    14521 tggagactcc ttgagaccgt aatgagcccg tttttccgtt ggcaagggcg cacctgcatc
    14581 gttatagtca gactcaggaa aagatgagcg ctcccagaat cccgcataca gcaaggacca
    14641 ggatggggtc gatcttgcct acaaaaacca tgaccgctgc tgcagcggct actgcatatg
    14701 aacgaggttc agccatcgac acctttgcta cggatattgc tgcggcagct atcaggcccg
    14761 caacagccgg tcgcatgccg accttgatcc tactaagcgt ggcggtgcgc tcatattttc
    14821 taacaagagc agcaagagcc aagacaatga ctattgatgg ggttatgacg cctagtgtag
    14881 ctacgattcc acctattatc ccggcaagct tgtaccctac aaacgtggcg ctattcacgg
    14941 ctatgggccc gggtgtaacc tcggcaattg cgaccatgtc cgtgaattcc gccatagtca
    15001 accagccgcg cttttgaata atgtcagatt caatcagcgg cagcatagca tacccgccgc
    15061 cgaacgtgaa aagcccaacc ttggcaaaac tgaggtatat gtggaggagg ttcatcttga
    15121 atctcctcca atcccggtca gaagacctgc caatgcagca atgacaacca ccagcgcagg
    15181 gctgatatga agcaaagaaa ccattatcgc cgcagccgcg gctatcgcca attctccggc
    15241 ggagcggatc accggttttc ctagttttat ggcagcggac gcgataagga ctgcaacagc
    15301 tgggcgtatg ccagagaaaa tcgcctggac cacatctagc gaggagattc gatcgaagac
    15361 cgatgctatc atcaagatta ttattataga tggaataatg cagcctgcta cagatgctgc
    15421 ggcacccgca aaacccctta gcgcatagcc tacatatacg gacgtattga cggctatcgg
    15481 gccgggtgcc gtctgcgcaa ttgctagcgc atcgatgaat tcatccttgg tgacccaacc
    15541 cttcctgtca accacgtcct tctgaattaa gggaaccatc acatatcccc cgcctatggt
    15601 aaacgcacct attctgaaga atgtccagaa catgtcccat aatgacgccg atttagaccc
    15661 gccatcgcca ttgtccgtct ctttttcaca gcatgtcatt ccagaaactt gtcgagaatc
    15721 cattctgatt tcctctcgtc atattgtaga tcaaagactc ttccatcctc agttcttatc
    15781 ctgtagttca cctttccgca tccgttcaac caccagtccc tctccttccg ctttccgccc
    15841 aacatagctt tatcgaaaga agtgaaatcc ctccatttgc tcatgaccgc ctccactttg
    15901 tgctccgtcc cccggtaata aaaggctttg ggatccccgc ttgcttcatc tgctcggaca
    15961 ctaatggggc ggcgaacaac ctttacgact ttcgggccct cccccgcaac agcccgagac
    16021 gagatctcat cgattgcgcg gcgcacgctc atcacgccca aaaacagacc cacaagaaag
    16081 ccgattatcg ttccccatgg tctagtctct tgctttgaat cgacatagct gcctattacg
    16141 aagcccataa ggatgaagct cgctatttct acggcaagaa gcacacgtgt cataacattt
    16201 cagctccatg cacagccatt cttctatccc tgtggtggat ccactattaa aggcagactc
    16261 ggttccggtt cggtcgctgt tggcgcggca gtcggaccaa gcactactac cttaggttga
    16321 ggcttgtagc gatcccgaga tatcagctct ctcctagcct cgacaccgtc gaccttgata
    16381 atgcgccata ctgacacgac gtagccaggc tttccatcac tttccaccct cacagatccc
    16441 gggggcgcca gataatcaac tcgttctatt gtgctgggtt cgagcctctc tacaacctcc
    16501 gtgtatatgc ttacatccat gttgtcgggc atatccccgt aaatcttgaa cgaaacagtg
    16561 tcactcccga catacgatcg tatcaagaca taatgggcgg tcgtgttctt aatcttgaga
    16621 tcgatggatc cgtacgaaac ggcagcatct cttcccatag acacatagct tatcggcaat
    16681 gaatggtttg tgcgggacac gggctccatc agcccgagca atgcagcgtt gtatagcgta
    16741 gttgaaacct gacatatgcc gccgccaacc ccgggaacaa gctgggaatc gaagattatg
    16801 tccgcttcca tgaaccctct ctcggggatc ctcggaccaa ccacgtcgtt gaaggacatc
    16861 acctcgccgg gagcaagcat cctattgttc agtttttgtg ccccaagcct gatgttattc
    16921 acccgattgg tttttctcgc gtcgaactta gttgtatatg cagatatcag gcggcgaatg
    16981 cccttggctt cgatggatgc tgtgctacat tttgcaggca tgcgcctcac aggaatctcc
    17041 aactcccgtg gcgaccgaga cgtagtggcc ttccttagcg cattgatgaa ctcgaccgtg
    17101 tccaacagcc agccatctga attatcttga ggcactccag gataaaatca ttctgtgtga
    17161 aaaaccgctg ggcctgacac ccgataatca agcccggttt ctggctctca atgccaaact
    17221 cagttacg
//
LOCUS       DAOSAI010000009        17160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_2301_length_17160_cov_3.796434, whole genome shotgun sequence.
ACCESSION   DAOSAI010000009 DAOSAI010000000
VERSION     DAOSAI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 17160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17160
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_17160_cov_3.796434"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /gene="rplD"
                     /locus_tag="PLM83_00765"
     CDS             <1..361
                     /gene="rplD"
                     /locus_tag="PLM83_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOK69977.1"
                     /translation="VVFGPKPRKYGYTIPKKVRRLALLSALSSMAQENAVYVVDAIDL
                     SEPSTKSMAALFEKIGAEKPLVVTDERSEVVEKSTRNIPGAMVLHVEGLNVYDIVNHT
                     ALVLTRDAVSRAEEAFA"
     gene            361..648
                     /gene="rplW"
                     /locus_tag="PLM83_00770"
     CDS             361..648
                     /gene="rplW"
                     /locus_tag="PLM83_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOK69978.1"
                     /translation="MAHPRDIIIGPIITEKSNALSAEGKYVFKVKRDANKIEIARAVE
                     KIFDVRVVAVNTINVPGKLKRQGRSEGMTPAWKKAIVTLQDGETIPLFEGV"
     gene            673..1503
                     /gene="rplB"
                     /locus_tag="PLM83_00775"
     CDS             673..1503
                     /gene="rplB"
                     /locus_tag="PLM83_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOK69979.1"
                     /translation="MGVKSFKPTTPARRQMTVATFEEITSSKPEKSLVVPLKKSGGRN
                     AQGRTTVRFRGGGHKRAYRVVDFKRDKDGVPGRIASIEYDPNRSARIALVNYADGEKR
                     YILAPLGLKVGDVIESGPDADIKPGNALPLSNIPVGTVVHAVELTAGRGAQLARSAGA
                     SAQIMAREGEYVTLRLPSTEMRMVRQECRATVGQVGNVEHENISMGKAGRSRWMGRRP
                     SVRGVAMNPVDHPHGGGEGRSPIGLKSPVTPWGVPTLGYRTRKAKASDRLIVRRRGKK
                     "
     gene            1536..1820
                     /gene="rpsS"
                     /locus_tag="PLM83_00780"
     CDS             1536..1820
                     /gene="rpsS"
                     /locus_tag="PLM83_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOK69980.1"
                     /translation="MGRSLKKGPYVHEALAEKVERLNSAGKKEIIKTWSRASMITPDM
                     LGHTIAVHDGRRHVPIYIVEDMVGHRLGEFAPTRTFRGHRQSDKTTQIKR"
     gene            1835..2173
                     /gene="rplV"
                     /locus_tag="PLM83_00785"
     CDS             1835..2173
                     /gene="rplV"
                     /locus_tag="PLM83_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOK69981.1"
                     /translation="MEAKAVARYVRMSPQKVRRVVDLVRGKSVDEALTILKYTPHIAA
                     EPVSKVVASAAANAENNLDLSRDDLYIAEAYVDEGPTLKRIRPRARGMANRILKRTSH
                     ITVVVREREG"
     gene            2177..2842
                     /gene="rpsC"
                     /locus_tag="PLM83_00790"
     CDS             2177..2842
                     /gene="rpsC"
                     /locus_tag="PLM83_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOK69982.1"
                     /translation="MGQKVNPIGLRLGINRTWESTWYSDKNYAELLHEDLKLRKYIKN
                     RLYAASVSRIQIERAANRIRVTIHTARPGMVIGRGGSEADALRRDLEAMTGKQVSVNI
                     MEIKHPELDAQLVAENIAAQLERRISFRRAMKQAMGRAERLGVQGMRVKSSGRLGGAE
                     IARTEGYSYGKLPLHTLRADIDYGFTEANTTYGKIGVKVWIYKGDVLPDKSGKARQGG
                     GAR"
     gene            2843..3277
                     /gene="rplP"
                     /locus_tag="PLM83_00795"
     CDS             2843..3277
                     /gene="rplP"
                     /locus_tag="PLM83_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOK69983.1"
                     /translation="MLMPKKVKYRKQQRGRMKGVAEKGTTVTYGEYGLQALEPGWITS
                     RQIEAARIAMTRYIRRGGKVWIKIFPDKPVSKKPAETRMGSGKGAPEYWVAVVKPGRV
                     MFEMGGVSEEIAREAMRLAGHKLPIKTQFVKREEAGGEADEG"
     gene            3267..3470
                     /gene="rpmC"
                     /locus_tag="PLM83_00800"
     CDS             3267..3470
                     /gene="rpmC"
                     /locus_tag="PLM83_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOK69984.1"
                     /translation="MKARELREMTPEQLDTRLEGLKEELFNLRFQLATNQLDNAHRIK
                     DVRRDIARVKTEMRERELKIARG"
     gene            3486..3746
                     /gene="rpsQ"
                     /locus_tag="PLM83_00805"
     CDS             3486..3746
                     /gene="rpsQ"
                     /locus_tag="PLM83_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOK69985.1"
                     /translation="METARNERKERVGFVVSDAMDKTRVVELQRTYRDPMYGKVVRRS
                     SKVKVHDEGNESRVGDKVLIMETRPISRDKRWRLVKIVEKAK"
     gene            3777..4145
                     /gene="rplN"
                     /locus_tag="PLM83_00810"
     CDS             3777..4145
                     /gene="rplN"
                     /locus_tag="PLM83_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOK69986.1"
                     /translation="MIQAQTVLNIADNTGAKKVMCFRVLGGGDRRYAGIGDIIIGTVK
                     EATPGGVVKKGDIVKAVVVRTKKELRRADGTYIRFDENAAVVIDANNNPRGTRIFGPV
                     ARELRDRNFMKIISLAPEVL"
     gene            4186..4518
                     /gene="rplX"
                     /locus_tag="PLM83_00815"
     CDS             4186..4518
                     /gene="rplX"
                     /locus_tag="PLM83_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOK69987.1"
                     /translation="MTISVKKVKKGDTVMVLQGKDAGRRGKVLRVLPREQKVIVENLN
                     IVKRHTRPSTANPQGGIIDKPAPMALGRVMVVCGKCNKPTRVGMELGESGKPMRVCKK
                     CGAKLDKK"
     gene            4552..5106
                     /gene="rplE"
                     /locus_tag="PLM83_00820"
     CDS             4552..5106
                     /gene="rplE"
                     /locus_tag="PLM83_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOK69988.1"
                     /translation="MEAPTPRLKEKYQNEVVPALMQKFEYRNVMEVPKVEKVIVNRGV
                     SDAIVDPKALDAAADELANITGQRPIITRAKKSIAAFKLRAGVPIGCKVTLRGARMYY
                     FLDKLFNIALPRIRDFRGVSPDAFDGRGNYTLGLREQLVFPEIRYDDVDKIRGMDITI
                     VTTAKTDEEAFELLKLMGLPVRLQ"
     gene            5119..5304
                     /locus_tag="PLM83_00825"
     CDS             5119..5304
                     /locus_tag="PLM83_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009753882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOK69989.1"
                     /translation="MARKALVEKWQKEPKYKVRQYNRCSICGRPRGYMRKFKMCRICF
                     RTLASKGEIPGVTKASW"
     gene            5340..5738
                     /gene="rpsH"
                     /locus_tag="PLM83_00830"
     CDS             5340..5738
                     /gene="rpsH"
                     /locus_tag="PLM83_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOK69990.1"
                     /translation="MVLTDPIADMLTRIRNANTVNHEVVEIPASRMKAELAQIMKDEG
                     YIRGFEVTRGDKYDVIKVHMKYGPNKQKVIHGLKRISKPGLRVYVKKDEVPKVLNGLG
                     TAVISTSRGLMTDRQAREAGLGGEVICYMW"
     gene            5750..6292
                     /gene="rplF"
                     /locus_tag="PLM83_00835"
     CDS             5750..6292
                     /gene="rplF"
                     /locus_tag="PLM83_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOK69991.1"
                     /translation="MSRIGKKPVPVPKGVEVELDGSIITVKGPKGALSQEIPARISVE
                     VADGEIRVTRPSDNRLDRSMHGLTRALIANMVHGVTEGFEKSLEIIGTGYRASKQGET
                     LVLQVGYSHPVEIVPENGIQIDVPSPTKITVRGIDKQLVGQTAANIRAVREPEVYHGK
                     GIRYAGERVALKAGKAGKIG"
     gene            6331..6699
                     /gene="rplR"
                     /locus_tag="PLM83_00840"
     CDS             6331..6699
                     /gene="rplR"
                     /locus_tag="PLM83_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOK69992.1"
                     /translation="MYNRVDRKEALARRHQRVRKDVSGTPERPRLSVFRSHAHIYVQI
                     IDDTTGHTLVAASSVEPDIRAKVGYGGNIAAAEEVGRVAAERAIEKGIRKVVFDRGGN
                     RYHGRVAALAEAARKGGLEF"
     gene            6718..7218
                     /gene="rpsE"
                     /locus_tag="PLM83_00845"
     CDS             6718..7218
                     /gene="rpsE"
                     /locus_tag="PLM83_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOK69993.1"
                     /translation="MAKINPDSLDLKERVVSINPVAKTTKGGRTRSFRALVVVGDENG
                     HVGAGLGKAYEVSEAIKKGVDDAKKNLVSVPMVGTTVPHEVTAKSGAGCVMLKPAGPG
                     TGVIAGGAVRAVLELAGYRDILTKSLGSNNPMSMVSATLVGLQSMRTAEQVARTRGKS
                     VEELAR"
     gene            7232..7417
                     /gene="rpmD"
                     /locus_tag="PLM83_00850"
     CDS             7232..7417
                     /gene="rpmD"
                     /locus_tag="PLM83_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOK69994.1"
                     /translation="MAKKLVVTLKRSTISRPKDQRATVRSLGLRKIGQTVEHDDTPVI
                     RGMINKVSHLVEVTEID"
     gene            7431..7874
                     /gene="rplO"
                     /locus_tag="PLM83_00855"
     CDS             7431..7874
                     /gene="rplO"
                     /locus_tag="PLM83_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOK69995.1"
                     /translation="MKLHELQPAAGAKRAPKRVGRGTGSGHGKTAGRGSKGQLSRSGG
                     GKGPGFEGGQTPLYRQLPKRGFTNKYKVEYAVVNVSDLERVFEAGDEVDRDALREKGL
                     VKGHDALVKVLGDGELTKSLTVRADKFSESAMSKIKAAGGSAEVV"
     gene            7875..9137
                     /gene="secY"
                     /locus_tag="PLM83_00860"
     CDS             7875..9137
                     /gene="secY"
                     /locus_tag="PLM83_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOK69996.1"
                     /translation="MLAALVNAWRVPDLRKKILYSAFLLFVYRLGAFIPAPGVDMAKV
                     AEVFQAVMGETGGGLLGLVDMFSGGSIQRLSVFAVNVGSYITASIVIELLTVVIPSLE
                     ELSKEGPEGRKKISQYTRYLAVALAVLQSYGYSAMFREAIRPGAVWTLLVIVSMTAGA
                     SFVIWLGEKISEKGIGNGISLIIFAGIVTQLPGNVKNVISLVKSGGTGWVFAIVYVLM
                     SLALVIGVVAISEGQRRVPVQYPKRVVGRRVYGGQSTHIPIKVNQAGVIPVIFASSVL
                     AFPGTLAQFIPGMAPGVRAFMNNVWAYNIVYVALIILFSYFYTGITFNPVEVADNMRK
                     YGGFIPGFRPGRPTAEYLDRVLTRLTFVGAIFLAIIALLPNITGRLTSNTVTFGGTGL
                     LIVVGVALDTMKQIEAQLIMRQYEGFLK"
     gene            9183..9830
                     /locus_tag="PLM83_00865"
     CDS             9183..9830
                     /locus_tag="PLM83_00865"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOK69997.1"
                     /translation="MRAVLMGPPSAGKGTQAVRLAAEFGVPHISTGDMFRAAIAAGAE
                     LGRRAQGFIEAGKLVPDDVTLGIVCERLAEADAMSGFILDGFPRTVPQAEGLEDILRA
                     QGVRLDAVVLIEVPEDVLVRRVTGRRVCEKCGRVYHMEWNPPPGSACECGGLVTQRKD
                     DFPETVKERLATYRTQTEPLRDYYASEGSLVVVDGTGDPDEVFRRITRALGRCGS"
     gene            9827..10573
                     /gene="map"
                     /locus_tag="PLM83_00870"
     CDS             9827..10573
                     /gene="map"
                     /locus_tag="PLM83_00870"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOK69998.1"
                     /translation="MIILKTAAEIEQMRKAGALVGQVLERLGRYVAPGVRTAELDAIA
                     EEMILAAGGTPSFKGYCGYPASICVSVNDEIIHGIPGKRVLVEGDIVSIDCGAILDGY
                     QGDAAMTFPVGDVSVEAEKLMRVTREALEAGIEMARPTMRLGDIGNAIQVHAESNGFS
                     VVRDFVGHGIGRRMHEDPQIPNFGSPGTGLRLRPGMVLAIEPMVNAGGHEVMIMDDGW
                     TTCTRDGSLSAHFEHTVAITDAGPEVLTLP"
     gene            10597..10893
                     /locus_tag="PLM83_00875"
     CDS             10597..10893
                     /locus_tag="PLM83_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HOK69999.1"
                     /translation="MSSGGPLLGQFVVSKMGRDRGRLFVVVGREGDGYVYIADGATHK
                     LGKAKRKNVKHLIVTEQVDEDIRAALEAGNSVTDEQLVTALRVYRPQERKEGDA"
     gene            10896..11114
                     /gene="infA"
                     /locus_tag="PLM83_00880"
     CDS             10896..11114
                     /gene="infA"
                     /locus_tag="PLM83_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOK70000.1"
                     /translation="MAKEDAIEVEGTVVEPLPNAMFKVELENGHTVLAHVSGKMRMKF
                     IRILPGDRVTIQLSPYDLTRGRIVWRYK"
     gene            11126..11239
                     /gene="rpmJ"
                     /locus_tag="PLM83_00885"
     CDS             11126..11239
                     /gene="rpmJ"
                     /locus_tag="PLM83_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOK70001.1"
                     /translation="MKVRPSVKKMCEKCKIVKRKGRVRVICENPTHKQRQG"
     gene            11255..11626
                     /gene="rpsM"
                     /locus_tag="PLM83_00890"
     CDS             11255..11626
                     /gene="rpsM"
                     /locus_tag="PLM83_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOK70002.1"
                     /translation="MARIAGVDLPRDKRIEVALTYIYGIGLTSSQKILAETGVNPDTR
                     VRDLTETEITQLREAIDRGYKVEGDLRREEAQNVKRLVDIGSYRGLRHRRGLPVRGQR
                     TKTNARTAKGPKKAIGGRKKR"
     gene            11661..12050
                     /gene="rpsK"
                     /locus_tag="PLM83_00895"
     CDS             11661..12050
                     /gene="rpsK"
                     /locus_tag="PLM83_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOK70003.1"
                     /translation="MAKRQARPRRRERKNIETGTAHIKSSFNNTIVTITDPAGAVVSW
                     SSAGAMGFKGSRKGTPFAAQQAADAAARGAIDQGMRSVEVLVKGPGGGREAAVRSLQA
                     AGLEVSMIKDVTPIPHNGCRPPKRRRV"
     gene            12074..12697
                     /gene="rpsD"
                     /locus_tag="PLM83_00900"
     CDS             12074..12697
                     /gene="rpsD"
                     /locus_tag="PLM83_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOK70004.1"
                     /translation="MARYTGSVCRLCRREGAKLFLKGDRCYTEKCAVARRAYPPGEHG
                     QGRKRTSEYGLQLREKQKLRRIYGVQEAQFARYFDMAERRRGITGENLLAILETRLDN
                     VVYRLGLGASRAEARQLVRHGHIYVNGKRVSIPSYELRAGDEISVRPGSKNLVHFKNL
                     AEAGAVTVPAWLLVNEDRLGGRVLSVPTRDQIDLDIQEHLIVELYSK"
     gene            12735..13718
                     /locus_tag="PLM83_00905"
     CDS             12735..13718
                     /locus_tag="PLM83_00905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOK70005.1"
                     /translation="MIESPKPRIDCQEDGDRYGRFVVEPLERGYGITLGNSLRRILLS
                     SLPGAAVTSVKIDGVLHEFSTIPGVYEDVTDIILNLKSLLVRLHTDVPKVIRLMAEGP
                     GDVVAADFEADAEIDILNPELHIATLGTVEAVNQDTGKTEERGAVLSLEAVVEPGRGY
                     VSSEKNKRPEQPIGVIPMDSIFTPVEKVNYTVENARVGGVTDYDRLVLEVWTHGTITP
                     KEAVSMAASMMNEHMKLFISLSEPEEEVEPEAEPEEDCRDRALEMVIEDLDLSVRSYN
                     CLKRAGINTVDELTHKTEEEMLKVRNLGRKSLEEVKQKLESLGLSLRQPDE"
     gene            13739..14125
                     /gene="rplQ"
                     /locus_tag="PLM83_00910"
     CDS             13739..14125
                     /gene="rplQ"
                     /locus_tag="PLM83_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOK70006.1"
                     /translation="MSRRKLSRTAEERRALFRGSVTSLFLHDKIITTEAKAKEIRPVA
                     ERLITWAKVGDLHHRRLAAKYVLDPVALQRLFDEIGPANIDRPGGYTRIIKLGQRRGD
                     GAPLAIIELVDMPGSSVSAKPAKAKK"
     gene            14132..15007
                     /locus_tag="PLM83_00915"
     CDS             14132..15007
                     /locus_tag="PLM83_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOK70007.1"
                     /translation="MKPVVRVENVSYAYDGGSQGQVEALLDVSLEIWPGEFVAIIGHN
                     GSGKSTLAKHMNALLLPTRGDVYVLGMNTRDESLLWDIRQTVGMVFQNPDNQIVATIV
                     EEDVAFGPENLGVPPDEMRRRVDEALALVDMTGYEKSAPHMLSGGQKQRVAIAGVIAM
                     RPRCIVLDEPTAMLDPVGRSEVLRTVRHLASNEGIAIVHITHFMSEAVYADRVIVMEE
                     GRVALSGTPREVFSQPATLRKLDLDVPQVTELAAQLRAAGVALPRYPLTVEEMADDIG
                     TLLGRGPCSSELQNH"
     gene            15033..15917
                     /locus_tag="PLM83_00920"
     CDS             15033..15917
                     /locus_tag="PLM83_00920"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOK70008.1"
                     /translation="MRGTPLARQALTDVNLSIDEGDLVAIIGKTGSGKSTLVQHFNGL
                     LKPDSGRVLVDGQDIWGKGVALRDIRRKVGLVFQYPEHQLFEETVALDIAFAPKNLGL
                     SDAQVDDAVREAMRIVDLDYEEFADRSPFELSGGQKRRVAIAGVIAMCPKVLILDEPT
                     AGMDPRGREDVLGEIQALHEKHGYTIVLVSHSMEDVARFAKRLVVMEAGKVVMDGDPR
                     EVFRHRQELIDIGLGVPQITDLMAELARRGYGFSTGAITVEEAREILLPPLLAASIES
                     TASAGPDPSEGRCGHGLR"
     gene            15904..16698
                     /locus_tag="PLM83_00925"
     CDS             15904..16698
                     /locus_tag="PLM83_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOK70009.1"
                     /translation="MDFANAAVGQYMPGDSFMHRLDPRTKMLTSLGVVIGLFVVSEPW
                     AYALLALPIMLGIMVSQVPLKLITRSLRPLTFIMVLTFALHLFMDPGEAIFSIGRITA
                     TYEGLRLGLITVVRLALLIMATSIVTLTTSPIALTDGLERLLKPFARLGLPAHELAMM
                     MTIALRFIPTLLEETDKIMKAQMARGADFESGNIAKRARAMVPILVPLFVSAFRRADE
                     LAVAMEARCYRGGEGRTRLRELAFSGADVAVLVVVGAYTVAVAVAL"
     gene            16700..>17160
                     /locus_tag="PLM83_00930"
     CDS             16700..>17160
                     /locus_tag="PLM83_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70010.1"
                     /translation="MRNIGLVMEYVGTGYQGFQVQPAFGRGGESRKGLEGRGGRLIRT
                     VQGEVERAIHRLTGESVRIVGAGRTDAGVHALGQVANFKTESRIPADRFGAALNSVLP
                     EDIRIIRSFEAPEDFNARYDAEWKIYRYLIVPEAARVPAARSGGALLSKRA"
BASE COUNT         4173 a         3885 c         5596 g         3506 t
ORIGIN      
        1 agtagtgttc ggccctaagc ctagaaagta tggttacacg attccgaaga aggttcgcag
       61 gttggcgctg ttgtcggcgc tgtcatcgat ggcgcaggag aacgcggtat acgttgttga
      121 cgccattgat ctatccgaac caagcacgaa gagcatggca gcgctttttg agaaaatagg
      181 cgctgaaaag ccccttgttg ttactgacga gcggagcgag gttgtggaga agtccactcg
      241 gaatattccg ggggcaatgg ttctccatgt tgaaggccta aatgtctacg atattgtgaa
      301 ccacacagcg ctggtactta cgcgcgatgc ggtttcaagg gcggaggagg cgtttgcgtg
      361 atggcacacc cgcgggacat tatcataggg ccgatcatca ctgagaagag caacgctctg
      421 tcggcagagg gaaaatacgt tttcaaggtt aagcgtgacg ctaacaagat agagatcgcc
      481 agggctgtcg aaaagatatt cgacgttcgg gtagttgcag taaacaccat aaatgttccc
      541 ggaaagctga agagacaggg acgttcggag ggaatgaccc cagcctggaa aaaggccatc
      601 gttaccttgc aggacgggga gaccatcccg ctcttcgaag gggtctaagt tcacgtaaag
      661 gaggtatgcg ggatgggagt caaaagtttt aagcctacta cgccggcacg gcggcagatg
      721 acggttgcca cgtttgagga gatcacgtcc tcgaagcctg aaaagtcgct ggtggttccg
      781 ctcaagaaga gcggaggacg gaatgcccaa ggcaggacca ccgttcggtt caggggcggc
      841 ggacacaaga gggcatacag agtcgtggat ttcaagcgtg acaaggacgg ggttcctggc
      901 agaatcgcgt ccatagagta tgacccgaac cgatctgcgc gaattgcact agtcaactac
      961 gcagatggcg aaaaacggta catcttggcc cctcttgggc tcaaggttgg cgacgttata
     1021 gagtcgggcc ctgatgccga catcaagccc ggcaacgcat tgccgctttc caacatccct
     1081 gtgggcacag tagtccatgc agtcgagctt acggccggaa gaggcgcgca gctcgccaga
     1141 tctgcagggg ccagcgctca gataatggcg agagaaggcg agtatgtcac tcttcgcttg
     1201 ccttcgacgg agatgaggat ggttcgccag gagtgccgag ctaccgtggg ccaggttgga
     1261 aacgtggagc atgagaacat ctctatggga aaggccggac gcagccgatg gatggggaga
     1321 aggccgagtg ttcgtggagt cgcgatgaac ccggtggatc acccgcacgg tggcggcgag
     1381 ggtaggtcgc ctattgggtt gaagagcccg gtcacacctt ggggagttcc gacactggga
     1441 tacaggacgc gcaaggccaa ggcttctgac aggttgatag tccgtcgccg gggcaagaag
     1501 taagaagtgg gttctgaaag gaggcgcgcc aatatatggg caggtcgttg aagaagggac
     1561 cgtatgtcca cgaggcctta gctgagaagg tcgaaaggct aaattcagcg ggaaaaaaag
     1621 agattataaa gacgtggtcc agagcgtcaa tgattacccc agacatgctt ggccatacca
     1681 tagctgtgca tgatggacgt aggcatgttc cgatatacat cgttgaggac atggtagggc
     1741 ataggctcgg agagtttgcg ccaacgagga cgtttagggg ccataggcaa tcggataaga
     1801 ctacacagat caagaggtag gaggtcgggc gcaggtggaa gcgaaagcgg tagcgcgtta
     1861 tgtgcgcatg tcgccacaga aggttcgcag ggttgtggat cttgtgcgcg gcaaaagtgt
     1921 tgatgaagcc ctgaccattc ttaaatatac tccgcacatt gcggcggagc cggtcagcaa
     1981 ggttgttgct tctgcagcgg caaatgccga aaacaacctc gaccttagca gggatgacct
     2041 ctatatcgct gaggcttatg ttgacgaagg ccctacgctc aagcggatac ggccgcgcgc
     2101 aaggggcatg gcaaacagga tcctcaagcg cacgagccac ataacggtag tggtcagaga
     2161 aagggagggt tagagtgtgg ggcagaaagt caatcctata ggtctgcggc ttggcatcaa
     2221 ccggacctgg gagtctacgt ggtattcaga taagaactat gccgagcttc tccatgaaga
     2281 tttgaagctc aggaagtata ttaagaaccg actttatgcc gccagcgttt cgcggataca
     2341 gatcgagcgt gcggcgaaca ggattagagt caccatccac actgcccgtc ctggtatggt
     2401 tattgggcgc ggcggttcgg aagcagatgc gcttcgcagg gacctggagg ccatgacggg
     2461 caagcaggtt tcggtgaaca ttatggagat caagcatcct gaactcgatg ctcagttggt
     2521 ggcagagaac atcgcagctc agctggagag gagaatctcc ttcagacgtg cgatgaagca
     2581 ggccatgggc agagccgagc ggcttggggt tcagggaatg cgggtgaaat ccagcggtcg
     2641 ccttggcggc gcagagatag ccaggacgga aggttacagt tatggcaagc ttccccttca
     2701 cacgttgaga gccgacattg attatgggtt cactgaggca aatacgacgt acggcaaaat
     2761 cggagtgaag gtatggatct ataaaggtga tgttctccca gataagtcgg ggaaagctcg
     2821 gcagggggga ggggctcgat aaatgctgat gcccaagaag gtcaagtacc gaaagcagca
     2881 gaggggacgc atgaagggcg ttgctgaaaa gggaaccacc gtcacctacg gcgagtatgg
     2941 ccttcaggcg cttgagcctg gatggataac gtcgcgacaa atagaggcgg cccgtattgc
     3001 gatgaccaga tacatcaggc gtggcggcaa ggtgtggata aagatatttc cagacaagcc
     3061 tgtgagcaag aagccggccg aaacacgtat ggggagcggc aagggtgcgc cggagtactg
     3121 ggtggcagtg gtgaagcccg gaagagtaat gtttgaaatg ggcggagtat ccgaagagat
     3181 tgcgcgtgaa gcaatgagat tggcagggca taagctgccg atcaagacgc agttcgtaaa
     3241 gagagaagaa gcgggtggtg aagccgatga aggctagaga gctgagagag atgacccccg
     3301 aacaactcga tactaggctg gaaggcctca aggaggaact cttcaacctc aggtttcagc
     3361 tcgccacaaa ccagctggac aacgcgcatc ggataaagga tgttaggcgt gatatcgcca
     3421 gggtgaagac ggaaatgcgg gaacgcgagc tcaagattgc acgagggtag agaggaggcc
     3481 aggtcatgga gactgctaga aatgagcgca aggagagagt cgggtttgta gtcagtgatg
     3541 caatggacaa gacccgagtg gtggaactcc agcgcaccta cagagatccg atgtatggca
     3601 aagtggtgcg tcggtccagc aaggttaagg tgcacgatga gggcaatgag agcagagtag
     3661 gtgacaaggt tctcatcatg gagacccggc cgatctcacg cgataagcgt tggaggcttg
     3721 taaagatagt agagaaggct aagtaacaag ggccctattg aagggaggaa cactctgtga
     3781 ttcaggccca gaccgtactg aatatagcgg acaatacagg cgccaagaaa gtgatgtgct
     3841 ttcgcgtctt gggcggcggg gaccgccggt atgccggtat tggcgacatc attattggca
     3901 ctgtcaaaga ggccacgccc gggggcgttg tcaagaaggg cgatatcgtc aaggccgttg
     3961 tcgttcgcac caagaaggag ctcaggcggg ccgacggcac atatatcaga ttcgatgaaa
     4021 acgcggcggt cgttatcgac gccaataata acccacgggg cacgcgaatc ttcgggcctg
     4081 tggcgcgaga gcttcgtgat cggaatttca tgaagatcat atcgcttgct ccggaggtcc
     4141 tctaggctac ggggcggtcc ccaggagcga ggaggtgtgg ataaaatgac gatatccgtg
     4201 aagaaggtca agaagggcga caccgttatg gtgctccaag gcaaagatgc aggcaggaga
     4261 ggcaaggtcc tcagggtgct gcctcgtgaa cagaaggtta tcgttgagaa cttgaacatt
     4321 gtaaagcggc atactcgtcc gagcaccgcg aatccgcagg gaggcatcat tgacaaaccc
     4381 gcgcccatgg cgctaggcag agtcatggtg gtctgcggca agtgcaacaa gccgacgcgg
     4441 gttggcatgg aacttgggga gtctggcaag cccatgagag tatgcaagaa gtgcggagcc
     4501 aagctggata aaaagtagga tagtcgatag gcctgagagg gggtcaatta tatggaggcg
     4561 ccgactccac ggctgaagga gaaataccag aacgaggttg tccctgcgct tatgcagaag
     4621 ttcgaataca ggaacgtcat ggaggttcca aaggtcgaaa aggtcatcgt caacaggggc
     4681 gtatcagacg ccattgtgga ccccaaggcg cttgacgccg ccgccgacga actagccaat
     4741 attaccggac agcgtccgat aatcacccgg gcgaagaagt cgatcgcagc gttcaaactg
     4801 cgggccggag ttcccattgg gtgcaaggta accctccgcg gcgcaaggat gtattatttt
     4861 ctggataagc tgttcaatat cgctcttccg aggattaggg acttcagagg ggtttctccg
     4921 gacgcttttg acgggcgggg aaactatacc ttgggtctta gggagcagtt ggttttcccg
     4981 gagatccggt atgacgatgt tgacaagatc cgtggcatgg acataaccat cgtgactact
     5041 gcaaaaacgg atgaagaagc tttcgagctg ttgaagctta tgggcctgcc agtcaggctg
     5101 cagtaaggag gggaagatat ggcgcgaaag gcgcttgtag agaagtggca gaaagagccg
     5161 aaatacaagg tgaggcagta taaccggtgc tcgatttgcg ggcgaccgag aggatatatg
     5221 cgcaagttca aaatgtgcag gatctgcttc cgaactctcg cctcgaaggg agaaatcccg
     5281 ggcgtgacca aggcgagttg gtgacagcag gggcacgcca cgagaggagg tagaggacga
     5341 tggtgttgac agacccgatc gcggatatgc ttactagaat acgcaacgcg aatacagtga
     5401 accacgaggt cgttgagata ccagcatctc ggatgaaggc agagcttgct cagataatga
     5461 aagatgaggg ctacatccgc ggctttgagg tcactcgggg agacaagtac gatgttatta
     5521 aggtccacat gaagtatggg cctaacaagc agaaggtaat acacggcctc aagcggataa
     5581 gcaagcccgg cctcagggtg tacgttaaga aggatgaggt tccgaaggtc ctgaacggcc
     5641 tgggaacggc cgtaatctcc acatctcgtg ggctgatgac agaccgtcag gcgagggaag
     5701 ccgggcttgg cggagaggtc atctgctaca tgtggtaggt ggtgtagaca tgtctagaat
     5761 aggcaagaaa ccagtgccgg ttcccaaggg tgtagaggtt gaactggatg gcagcataat
     5821 cactgtaaag ggccccaagg gcgctctgtc tcaggagatc cctgcgagga ttagcgttga
     5881 ggtggcggac ggcgagatca gggttacgcg tccatccgat aatcgacttg acagatctat
     5941 gcacggcctg acccgggcgc tcatcgccaa catggtgcat ggggttacag aggggtttga
     6001 aaaatccctc gagatcatag ggacgggata cagggcatcc aagcaaggcg aaacgttggt
     6061 gctgcaagtc ggctactcgc atcctgtgga gatagttccg gaaaacggta ttcagataga
     6121 cgttccgtct ccgacgaaga taacagtcag gggcatagat aagcagctgg tcgggcagac
     6181 tgctgcgaac ataagggctg ttagagagcc cgaagtatac catggcaagg gcataagata
     6241 cgcgggcgag cgcgttgcgc tgaaggctgg caaggccggc aagatcggct gatgctgcag
     6301 cttgcggcag aatgaaagga gcgacgtgca atgtacaata gggttgacag gaaagaggcc
     6361 ctggcccgcc gtcatcagcg cgttcggaag gatgttagtg gaacccccga acgccctagg
     6421 ctgtctgtgt ttcggagcca tgcccacata tatgttcaga taatagacga tacgacaggc
     6481 cacaccctgg tggcggcgtc ttctgtggag ccggacatca gggcaaaggt agggtatggt
     6541 ggaaacattg ccgcggcgga ggaagtgggc agagtagcgg cggagcgagc catagagaag
     6601 ggaatccgta aggtcgtatt cgaccgtggc ggcaatcgtt accatggcag ggtagctgca
     6661 ttggctgagg ccgcgcgcaa gggcggctta gagttttgag gcgggggagg gcagtaaatg
     6721 gctaagatca atcctgatag tctggatctt aaggaaagag tggttagcat caaccccgtg
     6781 gccaagacga ctaaaggcgg ccgaacgcgg agctttcggg cgctggtagt tgtgggtgac
     6841 gaaaacgggc atgttggggc tgggcttggg aaggcctacg aagtttcgga ggccataaaa
     6901 aaaggagtcg acgatgccaa gaagaacttg gtgtccgtcc ctatggtggg aactactgtt
     6961 ccgcatgaag tgaccgcgaa atccggcgcc ggatgtgtta tgcttaaacc cgctggccct
     7021 ggaacgggag tcatagctgg aggcgctgtt agggcggttc tcgaacttgc gggttatcgc
     7081 gacatactca cgaaatctct tgggtcgaat aacccaatga gcatggtcag tgctaccttg
     7141 gttggcttgc agtccatgcg caccgcggag caggtggctc ggacacgcgg caagtctgtc
     7201 gaggaacttg ctaggtagga ggggtcgaaa gatggcaaag aagctggttg tcacgttgaa
     7261 gagaagcacc atcagcagac ccaaagacca gcgggcgact gtgcgctctc tgggcctccg
     7321 caagattggc cagacggttg aacacgacga cacccctgtt atcaggggca tgatcaacaa
     7381 agtaagtcat ctggtcgaag ttactgagat cgactgagag gggtacagca atgaaactgc
     7441 atgaactaca gcccgcggcc ggcgcgaagc gcgcccccaa gagagtaggc cggggtacgg
     7501 gttccgggca cggcaagact gccggccggg gtagcaaggg ccagctgtct cgctcaggcg
     7561 gaggcaaggg ccctgggttt gaaggtgggc agacaccgct ttacaggcag ctgccgaagc
     7621 ggggcttcac caacaagtat aaggttgaat acgcagtcgt taacgtttcc gacctcgagc
     7681 gggtttttga ggctggtgat gaggttgaca gggatgcgct ccgcgaaaag ggccttgtga
     7741 aaggtcatga cgcgcttgtt aaggttcttg gagacggaga gcttacgaag tccctcaccg
     7801 ttcgcgctga caagttcagt gagagcgcaa tgtcgaagat caaggcggcg ggagggtccg
     7861 cggaggtggt ataggtgctg gcagcgctcg taaatgcttg gagggtcccg gatcttcgca
     7921 agaagatcct gtacagcgca ttcctgttgt tcgtgtacag acttggtgcc ttcataccgg
     7981 cgccaggtgt cgatatggcc aaggtcgcag aggtgtttca ggcggtaatg ggcgagacag
     8041 gcggcggcct tctgggcctt gtcgacatgt tttcaggagg cagtatacag aggctgtcag
     8101 tgtttgcggt caacgtggga tcctatatta ccgcgtcgat cgtgatagaa cttctgacag
     8161 ttgtcattcc gtcgcttgag gaattgtcca aggagggtcc ggagggacgg aagaagattt
     8221 cgcagtatac aaggtacctc gcggttgccc tggcggtttt acagtcatac ggatattcgg
     8281 caatgttcag ggaggcgatc cgccccgggg cagtctggac cttgctcgtg attgtcagta
     8341 tgactgccgg tgcctcgttc gtcatctggc tgggcgaaaa gatatccgag aaaggaatag
     8401 ggaatgggat ttctctgatc atttttgccg gcattgtcac gcagctgcca ggcaatgtta
     8461 aaaacgtcat tagcctggtg aagtcgggcg ggacaggttg ggtgtttgcg attgtatacg
     8521 tgttgatgag cttggcgctt gtaattggcg tagtggcaat tagcgaaggg cagcgcaggg
     8581 tgcctgtgca gtatcccaag agggttgtag gccggcgggt ttacggcggg cagagcactc
     8641 atataccgat caaagtgaac caggcgggtg ttatacccgt gatcttcgcg tcatcggtgt
     8701 tagcgtttcc cggcacgttg gctcaattca tacctgggat ggcacctggt gtcagggcgt
     8761 tcatgaacaa cgtatgggct tacaacatcg tatacgttgc tttgataatt ctctttagct
     8821 atttctatac gggcatcaca tttaacccgg tggaagtggc agataacatg aggaaatacg
     8881 ggggatttat ccccgggttc aggccgggac ggccgacggc ggagtacctc gaccgcgtgt
     8941 tgacgaggct caccttcgtg ggcgcaatat tcctggctat catagcgctt ttgcctaaca
     9001 taaccggacg gcttacatct aacactgtga cgtttggcgg tactgggctt ctgattgttg
     9061 taggcgttgc gcttgacacg atgaaacaga tcgaggctca gctgataatg aggcagtacg
     9121 agggcttctt gaagtaaggc tttggaatgc ccaagaaaat gaggatggga gtgcgtcgcg
     9181 agatgcgtgc agtgctgatg ggacctccca gcgcggggaa gggcactcaa gctgtcaggc
     9241 tggcagcaga gttcggcgtc ccgcatatat ccacagggga catgttcagg gccgcgatag
     9301 cggcaggcgc cgagcttggt cggcgggcac agggattcat agaagccggc aagctggttc
     9361 ctgacgacgt gactcttggc atcgtttgcg agaggttggc cgaagcggat gcaatgtcgg
     9421 gatttattct tgacggattt cctcggactg tgccgcaagc agagggactt gaagacatac
     9481 ttcgagccca gggcgtaagg cttgatgcag tggtgctcat agaggttccc gaggacgtct
     9541 tggtgcgaag ggtaaccgga cggagagtat gcgagaagtg tggtcgtgtg taccacatgg
     9601 aatggaatcc gcctccagga tccgcgtgcg agtgtggtgg tctggtcact caacggaagg
     9661 atgactttcc ggagacagtg aaggagcggc ttgcaacgta tcgaacgcag acagaaccgc
     9721 tgagggatta ttatgcctcc gagggaagcc tcgtcgtggt agatggaacg ggagatcctg
     9781 atgaggtttt caggcgcata acacgtgccc ttggaaggtg tgggtcatga tcattctgaa
     9841 aaccgcggct gaaatcgagc agatgcggaa agcgggcgcc ctggtcggac aggtgctgga
     9901 gagactgggc agatacgtgg ccccaggtgt ccgaacggcg gagctcgatg ccattgccga
     9961 agaaatgata ctggccgcag gcgggacgcc gtcgttcaag ggttactgtg gatacccggc
    10021 atcgatctgt gtgtctgtga acgacgagat catccacgga atacctggca aacgggtgct
    10081 ggttgagggc gatatagtat ctatcgattg cggtgcaatc ctcgacgggt atcaggggga
    10141 tgcagccatg acctttccag tgggagatgt ctcagttgaa gcggagaagc tcatgcgagt
    10201 gacgcgggag gcgcttgagg ctggaataga gatggcaagg ccgacgatgc gtcttgggga
    10261 tattggcaat gccatccagg tacatgccga gagtaatggg ttttcagtgg tcagggattt
    10321 tgttggccac ggcatcggac ggcgcatgca tgaggaccct caaatcccaa actttgggtc
    10381 gccgggcacg ggactacggc tcaggcctgg aatggtcctt gccattgagc ccatggtgaa
    10441 tgcgggcggc cacgaagtaa tgatcatgga cgatggctgg actacgtgca cgcgtgatgg
    10501 atcgctgtcg gcccactttg agcacacggt agccataact gatgctggcc ccgaggtttt
    10561 gactcttccg tagcatgcaa ggcaggtgta aggcagttgt cttccggggg tccattgctt
    10621 ggacagttcg tagtatctaa gatgggcagg gacaggggca ggctctttgt cgtagtcggc
    10681 cgtgaaggtg acggttacgt ttacattgct gacggcgcaa ctcacaagtt gggcaaggcg
    10741 aagcggaaaa acgttaagca cctgatagtg acggagcagg tggatgaaga tataagggcc
    10801 gcgctcgagg ccggaaactc ggtcaccgat gaacaacttg ttacggcttt gagagtgtac
    10861 agacctcaag agaggaagga gggggatgct tagttatggc aaaagaggat gccatagaag
    10921 ttgagggcac agtcgttgaa ccattgccaa acgccatgtt caaggtggaa ctggaaaatg
    10981 gccatacggt attggcgcac gtgtccggca agatgcgcat gaagttcatc aggattctgc
    11041 caggtgacag agtcacgatt cagctatcac cttacgatct gactcgcggc aggatcgtgt
    11101 ggagatataa gtaggagggt tagacatgaa ggtacggccg tccgttaaga aaatgtgtga
    11161 aaaatgcaag atagtgaaga gaaaaggccg cgttagagtt atttgcgaga accccacgca
    11221 taaacagcga cagggctagg aggtgcagcg tagcatggcg cgaatcgcag gtgtagacct
    11281 tccaagagac aagcggatag aggtagccct gacttatata tacggcatcg gcctcacctc
    11341 atcccagaag atcctggcgg agacgggcgt taaccctgat acgcgggtga gagatcttac
    11401 tgagaccgag atcacgcaat tgcgagaggc tattgaccga ggctataagg tagagggaga
    11461 cctgcgccgt gaagaagccc agaatgttaa gaggcttgtc gatataggct cttacagagg
    11521 gctgaggcat cgtcgcggac ttcccgtaag gggccagagg acgaagacga atgctcgaac
    11581 ggccaagggc ccgaagaagg ccattggcgg gcgcaaaaaa aggtgattag catggcgcgt
    11641 tgcggaaagg aggaacactg atggcaaaga ggcaggcaag gcctagaagg cgcgaacgga
    11701 agaatatcga aacaggaaca gcccatatca agtcgagctt caacaatacg attgttacca
    11761 ttaccgatcc ggcaggagct gttgtgtcgt ggtcatccgc tggcgccatg ggcttcaagg
    11821 gctcacgtaa gggtacgccg tttgcagcgc aacaggctgc ggacgccgcg gcgcgtggtg
    11881 ccattgatca gggaatgcgt tcagttgaag tgttggtaaa aggccccgga ggaggacgag
    11941 aggctgcggt aaggtcgctc caggctgccg ggcttgaagt tagcatgata aaggatgtta
    12001 cgccgattcc gcataacgga tgcaggccac caaagagacg gcgcgtgtag ccgagcgaac
    12061 aggaggtgcg ataatggcga gatataccgg atcagtgtgc cggctatgcc gacgtgaggg
    12121 agctaagctc ttcctcaagg gagatagatg ctatactgag aagtgtgctg ttgcccggag
    12181 ggcttacccg ccgggagagc atggtcaggg aagaaagagg accagcgagt atggtttgca
    12241 gcttcgcgag aagcaaaagc ttcgaaggat atacggagtg caggaagcgc agtttgcaag
    12301 gtattttgat atggccgagc gcagacgggg cattacaggc gagaacctcc tcgctatcct
    12361 tgagacccgg ctcgacaatg tcgtctatag actgggactg ggtgcctcta gggcagaggc
    12421 caggcaattg gtgaggcacg ggcatattta cgtgaatggc aagagggtgt ccataccatc
    12481 gtatgagctt cgtgcgggcg atgagatatc tgtgagaccg ggaagcaaga accttgtcca
    12541 cttcaagaat cttgccgaag caggcgctgt gactgtgccc gcatggcttt tagtaaacga
    12601 ggaccgtttg ggaggcagag ttctgtcagt gccgacaagg gatcagatag acctggatat
    12661 ccaggaacac ttaatcgtcg agttgtactc gaagtaatgc tgccccaaat gagaaggagg
    12721 gtgctgagcg cgctatgatt gaatctccaa agcctagaat cgattgccaa gaggatggcg
    12781 accgatacgg taggtttgtc gtggagcctc ttgaaagggg atacggcata accctgggca
    12841 actcattgcg caggatcctt ctgtcgtcat tgcccggggc cgcagtcacc tcggtcaaga
    12901 tcgacggggt gttgcacgag ttctcgacta ttcccggggt ttatgaggat gtgacagata
    12961 tcatcctgaa cctcaagagt cttctcgtga ggctgcatac agatgtgccc aaggtcattc
    13021 ggctcatggc cgaaggtccc ggtgatgtcg tggccgctga tttcgaggcc gatgcagaaa
    13081 tagacatact caatcccgag ctgcacattg ccacattggg cacagttgag gcggtgaatc
    13141 aggataccgg aaagacggaa gagcgaggtg cagttctgtc gcttgaagcc gtcgtggaac
    13201 ccggccgggg ttatgtgtct tccgagaaga acaagaggcc tgagcaaccc ataggcgtga
    13261 taccaatgga ctctatcttc actccagtgg aaaaggtgaa ctacaccgtg gaaaatgctc
    13321 gagtcggtgg agttaccgac tacgataggc tggttctgga ggtctggact cacgggacca
    13381 tcactccgaa agaggctgtc agcatggcgg cttcgatgat gaatgaacac atgaagctgt
    13441 tcataagcct ttctgagcct gaggaagagg ttgagccgga ggctgaaccc gaagaggatt
    13501 gccgagatcg ggcccttgaa atggtaatcg aagacttgga cttgtcggtg cggtcgtaca
    13561 attgcctgaa gagagcgggc ataaacactg tcgacgagtt aacccacaag actgaagagg
    13621 aaatgctcaa agtgagaaat ctgggacgca aatcgcttga agaggtcaag cagaagcttg
    13681 aatcattggg actgtcgctc cgacagcctg atgagtagtg taagggagga gagcgcagat
    13741 gagccgcaga aagctcagcc gtacagcgga ggagcggcgt gctcttttcc ggggctcggt
    13801 gacgtcgctg tttttacacg acaagataat caccacggaa gcgaaggcaa aagagatacg
    13861 cccagttgct gaaagactca taacatgggc aaaggttggc gaccttcatc acaggcgtct
    13921 tgcagccaag tatgtactcg atcctgtagc gctgcaaaga ttgtttgatg agatcgggcc
    13981 tgcgaacatt gatagaccgg gcgggtatac tcgcattata aagcttggcc aaaggcgtgg
    14041 ggatggagcg ccgctagcca ttattgagct ggtagatatg ccgggcagca gcgtaagcgc
    14101 gaagccggcg aaagccaaga agtgatgtgt tatgaaaccc gtagtgcgcg tcgagaatgt
    14161 gagctatgcc tatgatggcg gaagccaagg acaggtagag gcgcttctcg acgttagcct
    14221 ggagatttgg cctggcgagt ttgtggccat aatcggacac aacggatcag gcaagtcgac
    14281 actggctaag catatgaacg ccttgctcct tccaacacgt ggcgacgtct acgtgctcgg
    14341 gatgaacact cgcgacgaat cgttgttgtg ggatatacgt cagactgttg gtatggtgtt
    14401 tcagaaccct gacaaccaga tagtggcgac catcgtcgaa gaggacgtgg cgtttggacc
    14461 cgagaacctt ggagttccgc ctgatgaaat gcgccggcgt gtagatgaag cgctggcgct
    14521 tgtggacatg actgggtatg agaaatccgc cccccatatg ctttccggag gtcagaagca
    14581 gcgggtggct atagcaggcg tgattgcgat gagacccagg tgcattgtcc ttgacgagcc
    14641 cactgcgatg ctggatccgg tgggccgaag tgaagtactg cgtacagtgc gccatctcgc
    14701 ttccaatgag ggaattgcga ttgtgcatat aacacacttc atgagcgaag ctgtctatgc
    14761 agatcgtgtt atcgtaatgg aagaggggcg ggttgctctc tccgggacgc cacgagaggt
    14821 cttctcgcag ccggccacat tgcgcaagct ggacttggat gtcccacaag ttactgaact
    14881 cgctgcccag ctgcgagcgg caggtgtggc gcttccaagg tatccgctga cggttgagga
    14941 aatggcggac gacattggaa ctcttttggg cagggggccg tgcagcagtg agctccaaaa
    15001 tcattgaagt tcagaaccta tgctatacgt atatgcgagg cacgccattg gcgcgtcagg
    15061 cccttacaga cgtcaatctg tcgatcgacg aaggagatct tgtggcgatc ataggcaaga
    15121 ccggctctgg caaatctacc ctggtgcagc attttaacgg cctccttaag cctgattcag
    15181 ggcgcgtgct cgtggatggc caggatatct ggggcaaagg tgttgcgctg cgggatatta
    15241 gaagaaaagt aggcttggtt tttcagtatc ccgagcacca gctcttcgag gagaccgttg
    15301 ctttagatat cgcgtttgcc ccgaaaaacc taggattgtc cgatgctcag gtcgatgatg
    15361 ccgttcggga ggccatgcgc attgtcgacc ttgactatga agagttcgct gacagatctc
    15421 cttttgagct atccggcggg cagaagaggc gtgtggccat agccggcgtg atcgcgatgt
    15481 gtccgaaggt gttaatcctc gacgagccca ccgccggcat ggatccgcgt ggcagggagg
    15541 atgttctcgg agagatccag gcgcttcacg aaaagcatgg atacaccata gtcctcgtct
    15601 cccatagcat ggaggatgtt gcaagattcg caaagaggct cgtggtcatg gaagccggca
    15661 aggtagtcat ggacggtgat ccccgcgagg tattcaggca ccgtcaggag ctgatcgaca
    15721 taggcttggg ggttcctcag atcactgacc tcatggccga gctagccagg cggggatatg
    15781 gattctcgac tggtgcaatc acggttgagg aagcgcgtga gattctgttg ccgccgcttc
    15841 ttgcggcgtc tatcgagtcg actgcatctg caggcccgga cccatctgaa ggaaggtgtg
    15901 ggcatggact tcgctaatgc ggcagttgga cagtatatgc caggggattc cttcatgcac
    15961 aggctggatc cgcggacaaa gatgttgact tcgctcggtg ttgtgattgg tctgttcgtt
    16021 gtgagcgagc cgtgggccta cgcgttgctc gcacttccta taatgctggg aatcatggtt
    16081 tcgcaggtcc ctcttaagct gataacccgc agcttgcgac cgcttacgtt tatcatggtg
    16141 cttacttttg ccttgcacct gttcatggat ccaggcgagg cgatctttag catcgggagg
    16201 attacagcaa catatgaagg gcttcggctg gggttgatca cggttgtaag gctggcgtta
    16261 ttgatcatgg caacttcgat tgtgacgctg accacatcgc cgattgcctt gacagatggg
    16321 ctggagcgtc ttctgaagcc ttttgcgcgt ctcggattgc ccgcgcacga gctggcaatg
    16381 atgatgacta tcgcattgcg cttcatacca acattgctgg aagagaccga caagataatg
    16441 aaggcgcaga tggcgagggg ggccgacttc gaatcgggca acattgcgaa gagggcgagg
    16501 gcgatggtgc ccatacttgt gccactcttc gtgagcgctt ttcgcagagc tgacgagttg
    16561 gcggtggcta tggaagccag gtgctatagg ggcggcgaag ggcggacccg cttgcgcgaa
    16621 ttggctttca gtggagctga cgtggccgtg ctggtagttg tcggcgcgta cacggtggct
    16681 gtggcggtgg cattgtgaga tgcgcaatat cggtcttgta atggagtatg tgggcactgg
    16741 gtatcaggga tttcaagttc aacctgcatt tgggcgaggg ggcgaaagcc ggaaaggact
    16801 cgaaggacgt ggagggcggc tgattcgcac cgtgcagggc gaagtggagc gggcaataca
    16861 tcggctcaca ggcgagagtg tgcggatagt tggggcgggc cgaacagacg caggagtcca
    16921 tgccctaggg caggtagcaa acttcaaaac cgaatcaaga atccctgctg ataggtttgg
    16981 cgcagcgctc aacagtgtgt tgcctgagga tattcggatc ataagatcct tcgaggctcc
    17041 agaagatttc aacgcgcgtt atgatgcgga atggaagatt tacaggtact tgattgtgcc
    17101 ggaagcggcg agggttccgg cagcgcgcag cggtggcgcc ttgctctcga aaagggcatt
//
LOCUS       DAOSAI010000010        16472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_2418_length_16472_cov_3.917098, whole genome shotgun sequence.
ACCESSION   DAOSAI010000010 DAOSAI010000000
VERSION     DAOSAI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16472
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2418_length_16472_cov_3.917098"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLM83_00935"
     CDS             complement(<1..567)
                     /locus_tag="PLM83_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK70011.1"
                     /translation="MFKYGDDKGQLRCSFCGKAQEQVRRLIAGPGVYICDECIELCNE
                     IIEEESGISPDIDFSEIPKPHEIRAILDEYVIGQDDAKKTLSVAVYNHYKRISAAPHV
                     DDVELQKSNILLLGPTGCGKTLLAQTLAKILNVPFAISDATSLTEAGYVGEDVENILL
                     KLIQNADYDIERAEKGIVYIDEVDKIARK"
     gene            complement(583..1176)
                     /gene="clpP"
                     /locus_tag="PLM83_00940"
     CDS             complement(583..1176)
                     /gene="clpP"
                     /locus_tag="PLM83_00940"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOK70012.1"
                     /translation="MTLIPMVVEQTSRGERAYDIYSRLLKDRIIFVGGPIDDSMANLV
                     IAQMLFLQGEDPEKDINLYINSPGGVVTAGLAIYDTMQYVKPDVSTVCVGLAASAAAV
                     LLAAGAKGKRYALPYSRIMVHQPHGGTQGQATDIEIQAKEILRIKGITNEILSRHTGQ
                     PIERIDRDTDRDFYMSAQEAKEYGIIDSIFEGKAAEA"
     gene            complement(1414..2916)
                     /gene="tig"
                     /locus_tag="PLM83_00945"
     CDS             complement(1414..2916)
                     /gene="tig"
                     /locus_tag="PLM83_00945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOK70013.1"
                     /translation="MYKASVVDNDQGLVTLEITVSEDEVRRALGSAVRRLGQRYTVPG
                     FRKGKAPRLILENYVGREIIYDEAIRDFVPKAYEAALEETNLIPLEDPAFEGLDDADL
                     DSGEPLTFKARMTVKPEVKLGDYKSIKLDREKREVTEADIDRVLENLRDQRGEFVSTE
                     RTTAQAGDLVTVSIEAMVDGERLEEISVDDTQVPVGMGYLMEGIDEEIEGMTVGEPKS
                     VTAVLPEGFYIEELAGHDVTFNIIVKDIKVKQLPELDDEFAKDFGETKTLEELRNDIK
                     HSMMHHFLDEAKKDLGERALDALVTTSEVEPPKLLVEQEAYIAFDKLQETVEKEGLEW
                     EEYLEAKGQSEDDVYQEMLELAPASVKKALVIEALAKEENLFPTTDEVSIAVAQVMSY
                     AGAQSREILQKAIEDPEMRRVTTKALMREKVLAYLANTCDADPESAICQECAAREAEE
                     AKENAEKAQAESGTESRDEGIEQTSEADEPGSDEEMDGEAVAEETSSPEP"
     gene            complement(3093..3169)
                     /locus_tag="PLM83_00950"
     tRNA            complement(3093..3169)
                     /locus_tag="PLM83_00950"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3133..3135),aa:Arg,seq:tcg)
     gene            complement(3261..3337)
                     /locus_tag="PLM83_00955"
     tRNA            complement(3261..3337)
                     /locus_tag="PLM83_00955"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3301..3303),aa:His,seq:gtg)
     gene            complement(3576..3968)
                     /locus_tag="PLM83_00960"
     CDS             complement(3576..3968)
                     /locus_tag="PLM83_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70014.1"
                     /translation="MRLCLAPTVLEETSTNGVIVADVTIGELVYDGKLLDALEELDAP
                     VRFIVEEGMFAERLRNLLWTLPRSSRHVVELGIGLSNITPTGYIGADECIAGTCHFGI
                     GDNRFYGGRNASPIHLDVVVDRPRIKRY"
     gene            complement(3969..4415)
                     /locus_tag="PLM83_00965"
     CDS             complement(3969..4415)
                     /locus_tag="PLM83_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70015.1"
                     /translation="MQRCKRAVNTVIKTWCPLSRDDRTFIARLIVAEALCGLCKELGC
                     DTGICVFSKKEAEHSIANVPSELSDLGPMYAAFVLLSEAFYFGENGLAVLFPALRRPE
                     WLRSRYIFFRSRIPERALLETDRIAMKYALYASGDRLNVEAPGGLI"
     gene            complement(4458..5546)
                     /locus_tag="PLM83_00970"
     CDS             complement(4458..5546)
                     /locus_tag="PLM83_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOK70016.1"
                     /translation="MGELDLLVSLMIRTILFVGSVFGVGFFAASESAFLSMNKWAIDK
                     LAEEGDKNALIMRRLTEDSAKTVSAVLVGTNVLSTLSSVLAVSILLLVGKSGTRAIAI
                     ASIVTTAIILIFSELTPKTYAAANPTATALKVAVPLSWAITLLGPAASFLAWVPSQIA
                     ALFRRKSNAGNESPDEIIRAALDAAEDDGSVVPTETEVIHGVLDSKRKFVRDIMIPID
                     RTVALDYDSTLEDFMEAYLKERYSRMPIIASFCGKVLGIAHVKDVMKEIVAGGDPAAK
                     KALSVANTACLVSADAPALDAFNEIRSRKAHLAVVVQRGIAVGIVTIEDFLEEIVGDI
                     PEDASGHVVKSTYGTMALAVTVEGAENA"
     gene            complement(5539..6843)
                     /locus_tag="PLM83_00975"
     CDS             complement(5539..6843)
                     /locus_tag="PLM83_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOK70017.1"
                     /translation="MDDPSSSTYLFAFICLVLSGAFSASEAAILSSDEARLKSRYSSD
                     PRLRSILAVNKSPAPVMSSILLGNTLVNAMFSALAAFIIHSIVPGDAGLADAIATAAA
                     TVMLLVFAEVSPKLVSGGNPEAVALWASSWLLGLARALAPVSSVLTALSGHLVACLPQ
                     PDTAEEDFGELRFLAAVEYGEEAGAISGLEKEMIWGAMETHDLLAKDIMTPRPRIVAA
                     SEDQSTAEALDLMLKYGFSRIPVYADSIDNVTGILNMKDIALLISCSPNDWRERLDLM
                     PCVQLAVRPYFTHERKRVSDLLIEMRLKGIHTAIVVDGHDGVSGLVTLEDALEVIVGD
                     IQDEYDEETPELVMSEAGCCHVVGSVRLADLAEAFDVELPTEEFDSVADFIMACLDRV
                     PSEGDAYSLVGPRLTLEVVEMDGPRIKRAIVRLSEMETGRHG"
     gene            7104..8489
                     /locus_tag="PLM83_00980"
     CDS             7104..8489
                     /locus_tag="PLM83_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOK70018.1"
                     /translation="MTENMDALNGFFHRFHRAKLNPSQVLVTGFAAVIIIGAILLSLP
                     AASANGKSIRFIDAIFTATSATCVTGLVVLDTATSFSLFGQIVIIILIQIGGLGFMAL
                     ATAIALFLGRKITLRGRILLQESLNQFSIAGLVRLTRYLLLTTFIVEGVGAIILSLRF
                     SSIMPTSKAIYYGIFHSISAFCNAGFDLFGSITGPFSSLTYWASDPTVVLVIGSLIII
                     GGLGFPVIVELVGHRRGNRLSLHARLVLTVTLWLLAIGTAATFGLEFSNPDTLGKMSP
                     AGKVLGAVFQGITPRTAGFNTLDISKMRSSTLFIFVILMFIGASPSSTGGGIKTTTMG
                     VIIAAILSTIRGSDDVEINDRQISQSLVFRAITIASLALALVTGVTLVLSATEEASFL
                     QVLFEAASAFGTVGLTTGITPNLTDFGRVLIMITMFSGRLGPLTVMLALAQRQKPLAN
                     VHLPEEKVLIG"
     gene            8486..9091
                     /locus_tag="PLM83_00985"
     CDS             8486..9091
                     /locus_tag="PLM83_00985"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOK70019.1"
                     /translation="MRIYLVRHGQTVRGREETFRGRMDAPLDDMGRKQAAAVATALRD
                     VKLVAVYTSPLSRSVDTARAIAVSHGINIIVRDALTDIDVGEWEGLSTDRIRSDYGRL
                     YLDWQTGPDSFTFPGGESLSDVSDRAMAAMGKIVSSHEHDDELAIVSHRVLTKLILLN
                     MLHAPVSSFWKLSQDPCCINVVDYTEAGYVVESVNCISHLA"
     gene            complement(9866..10438)
                     /locus_tag="PLM83_00990"
                     /pseudo
     CDS             complement(9866..10438)
                     /locus_tag="PLM83_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            10818..12023
                     /gene="malE"
                     /locus_tag="PLM83_00995"
     CDS             10818..12023
                     /gene="malE"
                     /locus_tag="PLM83_00995"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF007011.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HOK70020.1"
                     /translation="MKRSLAIAMCISMLLGLATCASASQEGKLLIWADGTRAPVMEDL
                     GKKFTAKYKVPVEVQELGFGDIRDQLGVAGPAGKGPDIIIGAHDWLGELVTNGLIEPI
                     DLQAKEKDFLKVSVEAFTWGSTLYGVPYAVEAVGLFYNTKLVPNPPKTWDDLVAICKK
                     LTDKDKKQYGFLLPIPDPYHTFPIMSAAGGYVFGVNKDGTLNPLDVGLNNAGAVRGLS
                     LLDKLVEDGLMPQSMDYNTMMGLFKDGKVGMMLTGPWALADLRAAKVPYGFTTIPKID
                     GKDPRPFVGVQGFMISSFSKNKMLAKAFLNEYVATADTMMALFKQDPRPPAYLATARA
                     VSSDADIRATGDSAAAGIPMPAIPEMSAVWTAWANAIELTFNQKLEPKKALDDATAQI
                     ISAIKASKK"
     gene            12133..13710
                     /gene="malF"
                     /locus_tag="PLM83_01000"
     CDS             12133..13710
                     /gene="malF"
                     /locus_tag="PLM83_01000"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008232.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HOK70021.1"
                     /translation="MKVATRRAPVDMPIPGLIAKVALLGILNALAIWAIFVLIGESAY
                     WIAVMLAAGCALIDWVFLTPRLFPWRYLIPGLIFMAAMVVYPICYTVAVAFTNYGTGH
                     LLTKEQVIENYLSRATLQEDAPEYPAAMYKSHDGRFAVVLIDPKGEKLLGVDGEVLPL
                     GEVQLPVEYADSDGVPESIGPYQRLGTLELFQHMSAVEALQFHFEDFILKMRNVDRFA
                     TYKPQYSYDSNLDALIDTTTGKVYTATQGTFLADDGEALDVGYRAPVGWSNFARLIRS
                     PQVSGPFFRVFVWTFEWAALSVVTTFILGLLLAVLLNDQKMKFKGFYRVLLVIPYAMP
                     AFISALIWRGLLNAEVGLINDMLQFLFNVRVPWLQDPFWAKVALIIVNLWLGFPYMML
                     VSLGSLQSIPDELYEASRVDGATGWQQFWHVTFPLLLVSVAPLLVSSFAFNFNNFGVI
                     YLVTQGRPAIPGAQTPAGATDILITYTYHLAFESGSGTDYGLASAVTILIFLIIGTLS
                     WFNFRFTGALEEVHENV"
     gene            13703..14560
                     /locus_tag="PLM83_01005"
     CDS             13703..14560
                     /locus_tag="PLM83_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK70022.1"
                     /translation="MYKGFLEPAYLWRHIVIWLCIAFAILPVLWIISASFDPSNTIIG
                     QKLIPSNPSLENYRKLFNDPTHPFGRWMLNTLKVSFITSALVVIMTSLAAYSFSRFRF
                     RGRKTGLFSILLIQMFPQMLAMVAIYLLLLWIGEHFPTFGLNTHGGLILVYLGGAMGV
                     NVWLMKGYFDTIPTSLEEAATIDGATRFQCFYKVILPLSRPILVVIFFLQFIGTYSEY
                     VLAKVLLSSNDVLTLAVGLQSFIGDQYAKRWGVFSAAAIIGSAPILTLFALLQNELVS
                     GLTRGAVKE"
     gene            14598..16445
                     /locus_tag="PLM83_01010"
     CDS             14598..16445
                     /locus_tag="PLM83_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOK70023.1"
                     /translation="MQKHAIWHVPYDAMAYPISDDRLEIVLRAARGDLRRVEAVYTDR
                     YTPHARTSSTRLWIAGSDGLFDYWRGSMKLQYGRFMYYFELQDDEKRICYGEGGFSDE
                     PPQDEPWTWCFQYPCIWYHGGMDDPPKWAQDAVVYQIFVDRFANSDRANDPPGVSRWG
                     TLPTPRSFSGGDLAGIIEKLDYISSLGVSCLYLTPIFLSSSNHKYDTIDYYHIDPHFG
                     NEDTARHLVDQCHARGIRVVLDAVFNHSGYDFFAFRDVVKKGDASPYWKWFNIHGYPV
                     RTRPKPNYETFGPGIWQMPKFRTDNPEVRDYLISAAEHWTRTLDIDGWRLDVADDVDQ
                     GFWREFRTRMRQIKPDILIVGEILHDASPFLRGDQMDSVMNYPWRELCLDYFARRRIG
                     TADFADGITATAMHYRRQTQYAMWNLLGSHDRQRFLTACRGDTARLMLATTFQFTYTG
                     TPYIYYGDEIGLKGGEDPDCRKCMPWEEDLWDLDLLEHHRALARLRAEHPVLRRGDFA
                     SLIADGPDSPYVYARWDDDACIVVALNNTEEPQTVNTKLVAERMRKTHELEQWAGMPM
                     KQIFSLHGDNTGQGRSDNTQASMSAVGENCGRHVVGLPGMGAAVFQVQS"
BASE COUNT         3849 a         4680 c         4071 g         3872 t
ORIGIN      
        1 ctttctagct atcttgtcta cctcatcaat gtaaacaatt cccttttccg cgcgctcgat
       61 atcgtagtcg gcgttctgga tcagcttgag caaaatgttc tcgacatcct cgcccacata
      121 gccagcctcc gtaagagaag tggcatctga tatggcaaaa ggcacgttga gtatcttcgc
      181 aagggtttgc gcgagcaatg ttttgccgca gcccgtgggg ccaagcagca agatgttgct
      241 tttctgcagc tccacgtcgt cgacatgggg tgcagcgctg atcctcttat aatggttgta
      301 cacggcaacc gagagcgtct tcttagcgtc atcttgccct atcacgtatt catcaagaat
      361 ggcgcgtatt tcgtgcggct ttgggatctc gctgaaatcg atgtcaggac tgattcccga
      421 ctcttcctcg atgatctcat tgcacagctc aatgcactca tcgcagatgt aaacgccagg
      481 accagcgatg agccttctta cctgctcctg ggccttgcca caaaaggagc accttagctg
      541 acccttatca tcaccgtact tgaacatgat accacccctc ggctaagcct ctgcagcctt
      601 gccctcgaaa atgctgtcaa tgattccata ttccttagct tcctgggctg acatgtaaaa
      661 gtcgcgatcg gtgtcgcggt cgatccgttc gattggctgc ccagtgtggc gcgagagaat
      721 ctcgttggta attcctttaa ttcgcaggat ctccttggcc tgtatctcta tgtctgtagc
      781 ctgaccctgt gtgcctccat gaggctgatg aaccattatc ctggaatagg gcagtgcgta
      841 acgcttgccc ttcgcgcccg cagcaaggag tacggctgcc gcgctcgccg caagcccgac
      901 gcataccgtg gaaacatcag gcttcacgta ctgcattgta tcatatatcg ccagcccagc
      961 agtgactact ccgcctgggc tattgatgta aagattgata tccttctcag gatcttcgcc
     1021 ctgcaggaaa agcatctgcg ctatgacaag attcgccata ctgtcatcta tcgggccccc
     1081 aacgaagatg atcctgtctt tcagaagcct ggagtaaata tcatatgctc gctcaccacg
     1141 gctagtctgt tcaacaacca taggtatgag ggtcatgtcc atcacctctc tggtttcaat
     1201 ccacgcccct cagcgagagg caaaggtgga gtatgcaact cttgccagga acgtgtgctg
     1261 agccatccag cagagtccag attattctgc tgctttaggc cgcgaataat actccatgta
     1321 actctatgcg tgcaactgcc ttcgaagact tggccatatc gcccgcatcg gaagcggtcc
     1381 tgaagcgctg atcggctatt tcccgatttg cgcctatggc tctggtgacg acgtttcttc
     1441 cgctacggct tctccgtcca tttcctcatc tgaacccggc tcatcagctt cactggtctg
     1501 ctctatgccc tcgtcacgcg actcggtccc agactcggcc tgagccttct cagcgttttc
     1561 cttcgcttct tccgcttcgc gtgcagcaca ttcctggcat atagcagact ccggatccgc
     1621 gtcgcatgta ttggctaaat atgccaagac tttttcacgc atcagtgctt tagtcgtaac
     1681 cctacgcatt tcgggatctt ctatcgcctt ctgcaatatc tcacggcttt gagcacccgc
     1741 ataactcatt acctgagcaa cggcaatgct cacttcgtca gtagtaggga aaaggttttc
     1801 ctctttggcc aaagcctcaa ttaccaatgc tttcttgaca gaggccggcg ccaactcgag
     1861 catctcctga tacacgtcat cttccgattg accctttgcc tccaggtatt cctcccattc
     1921 gagcccctcc ttttccactg tctcctgcag cttgtcaaat gcgatatatg cctcttgctc
     1981 taccaggagc ttgggaggtt cgacttctga ggtcgtcacc aacgcgtcga gcgcgcgttc
     2041 acccaaatcc ttcttggcct catcaaggaa atggtgcatc atggaatgct tgatatcatt
     2101 gcgcagctct tcaagggttt tcgtctctcc aaaatccttg gcaaactcgt catccaactc
     2161 aggcagttgc ttgaccttga tgtcttttac tattatgttg aacgtaacat catggcctgc
     2221 gagttcctcg atataaaaac cttcagggag aacagcagtg acagatttgg gttcgccaac
     2281 cgtcatgccc tcgatctctt catctatgcc ttccatgagg taacccatac ccacgggaac
     2341 ttgcgtgtca tcaactgaga tctcttccaa acgctcccca tcaaccatgg cttcgattga
     2401 gacggtcaca aggtccccag cttgagctgt ggtacgctct gtagaaacaa attcgcctcg
     2461 ctgatcacgc agattctcaa gcacgcggtc gatatcggct tcagtcacct cgcgcttctc
     2521 ccggtcgagc ttaatagact tgtaatctcc cagctttacc tcgggcttca ccgtcattct
     2581 cgccttgaac gttaacggct cgcctgaatc gaggtcagca tcgtcaaggc cctcaaatgc
     2641 cggatcctcc agaggaatca gatttgtctc ctcgagagca gcctcatagg ctttcggcac
     2701 gaaatcacgg atagcttcat cgtatatgat ctctctgcca acgtagttct ccaatattag
     2761 gcggggcgct ttacccttcc ggaaacccgg gaccgtatat cgttgcccca gtctgcggac
     2821 cgcgcttcca agcgcgcgac gaacctcgtc ttcgcttacg gttatctcca gagtcactaa
     2881 tccttgatca ttatcgacta cactggcttt atacatgagt acacctcgct gtcccgagca
     2941 tccttcgggt aacctattcg acgatgcgta agttgattcc tttgaaaact ttaattcaca
     3001 atagtatgcc ccggcgagcg gctgttcaag ccaccaccgg ggcatcaatg ccaatccccg
     3061 atatgcaccg gttacactct ggcaaacaaa aatggcgcgc ccggcagggc tcgaacctgc
     3121 ggcctatgga ttcgaagtcc atcgctctgt ccaactgagc tacgggcgcc cgcttcatgc
     3181 ccgcgacaaa gtatagcata acccaaagcg catgtaaagc aatagaccct ggatatccca
     3241 gggcctcaca atcgaattga tggggtggat gatgggactt gaacccacga ccctcagagc
     3301 cacaatctga tgctctagcc aactgagcta catccaccac gttgcttaag aattttatca
     3361 taggcgtacc ccatggtcaa gggtcccgca cgactttgtt acgcgttttg ccaaacgcaa
     3421 ttctgtgcca agcacagtcc tatgaccaca attgcttcgc tagagcgaac ttccgtgttc
     3481 aatgtcactt tttatgaggc aacaagtgac tcccaaaaag tcttcagctt tgctccagac
     3541 tgtaagcggc ttgccaagtc tcaaacacaa cacagtcaat agcgcttgat acggggccga
     3601 tccacaacta catctaggtg tatgggcgag gcattccttc ctccataaaa cctattgtcg
     3661 ccgattccaa aatggcatgt gcctgcaatg cactcgtcag ctccgatgta acccgttgga
     3721 gtgatatttg acagtcctat tcccagctca accacgtgtc gactggatcg gggtaatgtc
     3781 catagcaggt tcctgaggcg ttcggcaaac atgccctctt ccactataaa tcgaacaggc
     3841 gcatctagct cttccaaggc atccaacagt ttgccgtcgt acacgagctc cccaattgtc
     3901 acatcagcta ctataacgcc gttggttgaa gtctcttcca aaacagttgg cgccaggcaa
     3961 agtcgcatct atatcagtcc ccctggcgcc tcaacgttta gcctgtctcc gcttgcatac
     4021 aatgcgtatt tcatagctat tcgatcagtt tcgagaagcg ctctctcagg gatccgcgat
     4081 cgaaagaata tataccgaga acgcagccat tcgggacgtc tgagcgctgg gaaaagtacg
     4141 gcaagaccgt tctccccgaa atagaaggct tcggagagca gcacgaaggc cgcatacata
     4201 ggcccaaggt ctgacagttc agatggaacg tttgctatcg aatgctcggc ttctttcttc
     4261 gaaaacacgc atatccccgt gtcacatcct agttccttgc atagcccaca caaggcttca
     4321 gcaacaatca gtctggcaat gaaagtccgg tcatctcggg ataagggaca ccaggtcttg
     4381 atcaccgtat tcacagcacg cttacatctt tgcatggacg cttccaaaac tgatttgtgc
     4441 gatagttctg gctagcttta ggcgttctca gcgccttcta ctgtcacagc cagggccatt
     4501 gtgccatagg tcgacttcac gacatggccg gatgcatctt ccggaatatc gcccactatc
     4561 tcttcgagga aatcctctat ggtcacaatg cccacagcga ttccgcgctg gaccactacg
     4621 gccagatgag ccttgcggct ccgtatctcg ttgaaagcgt cgagtgccgg agcatcggca
     4681 gataccaaac aggcggtgtt tgccaccgac agagccttct ttgcagccgg atcgccgccc
     4741 gctacaatct ccttcataac atccttcaca tgtgcaatgc caagcacttt gccgcagaat
     4801 gaagcgatta tcggcatccg gctgtagcgt tctttcaggt aggcctccat gaaatcctcc
     4861 aaagtggaat cgtaatccaa agcgacagtc cgatcgattg ggatcatgat gtcgcgaaca
     4921 aacttccttt tggaatccaa aactccatgg atcacctcgg tctcagtcgg aacaactgac
     4981 ccgtcgtcct cagcagcatc aagagccgct cgaatgatct catcaggaga ctcattgcca
     5041 gcattcgatt ttcgcctaaa caaggcagcg atctgcgagg gaacccaagc aaggaatgat
     5101 gccgcagggc cgagcagagt aattgcccag gataacggaa cagcgacctt cagggcagtg
     5161 gctgtgggat ttgctgccgc atatgtcttc ggagtcagct cagagaaaat cagtataatc
     5221 gcagttgtca ctatcgatgc aatagctatc gccctcgtcc ctgatttccc tacaagcaac
     5281 agaatggaaa ccgccaggac agatgagagt gttgacagga cattcgttcc gaccagtaca
     5341 gctgatactg tcttggcgga atcctctgta agcctgcgca tgatcaacgc gttcttgtcg
     5401 ccttcctccg caagcttgtc aattgcccac ttattcatgc tcaagaacgc gctctcagac
     5461 gccgcaaaga agcccacacc aaacactgaa ccaacaaaca gaatagtccg gatcatcaaa
     5521 gaaaccaata agtccaactc acccatggcg tcccgtctcc atctccgata gacgcactat
     5581 ggcccttttt atacgcgggc cgtccatctc cacaacctcc aaagtcaatc ttggccctac
     5641 aagagagtag gcgtccccct cggaaggcac cctgtcgagg catgccataa tgaagtcggc
     5701 tacggaatca aattcctccg taggcaactc cacatcaaag gcctctgcaa gatcagccag
     5761 cctaacactt ccaacgacat ggcaacaccc tgcttccgac atcaccagtt ccggagtctc
     5821 ctcgtcatat tcatcttgaa tgtccccaac tataacctcg agagcgtctt cgagcgtgac
     5881 caggccagat acgccatcat ggccgtccac aacaatcgcc gtatgtatgc ccttcagacg
     5941 catttcgatc agcaaatcgg agactctctt tctttcatgg gtaaagtagg gtctcactgc
     6001 cagctgcacg catggcatga ggtccaatct ctcacgccag tcattggggg agcagcttat
     6061 gagcagagca atatccttca tgttgagtat tccagtcaca ttatcgatgg aatccgcgta
     6121 caccggaatg cgggaaaaac cgtatttcag cattaagtca agtgcctctg cggtgctctg
     6181 atcctcactc gctgccacaa tacgcggcct cggagtcatt atgtctttgg ccaagaggtc
     6241 atgggtttcc atggcccccc atatcatctc cttttccaga ccggatatgg cgccagcctc
     6301 ttcaccgtat tcaactgcag ccagaaatcg cagttccccg aaatcctcct ccgcagtgtc
     6361 cggttgggga aggcatgcca ccaaatgccc cgacaatgct gtcagcacgc tagataccgg
     6421 cgccagcgca cgagccaaac ctaacagcca ggatgaagcc caaagcgcaa cggcttctgg
     6481 gttgcctcca cttaccaatt tagggcttac ctctgcgaat acgagaagca tcaccgtagc
     6541 cgctgccgtc gcaattgcgt cagcgagccc agcatcgccc ggtacaatag agtgaattat
     6601 gaatgccgca agtgcagaga acattgcgtt taccagagta ttgccaagaa gaatagacga
     6661 catgacagga gcagggctct tattcacagc aagaatcgaa cggagtcggg ggtcggatga
     6721 atatcgactc ttgagccttg cctcatcgga tgagagaatc gcagcctcgc tcgcggaaaa
     6781 agctccagac aacaccaagc agataaaggc aaacaagtag gtactgctac ttgggtcgtc
     6841 cacccatcat cacctctcag tgtcgataag tagtatgaca tattttatac tcttctcaat
     6901 cttgtttcaa caccataaaa tcactctcta cgtgccacac ccactaagtg caccaagcca
     6961 tgagctggat tgggtccgat tctgcgtcca gcttgcacct ggttacgcag ttcgcgaggg
     7021 tagaacggcc gtcaatttga tacaattgac acgcgaacac cccgatgata ggattgtttg
     7081 cattcaggag agtgaaagcg ggtatgactg aaaacatgga tgcgctcaac ggattcttcc
     7141 acaggtttca tcgtgctaaa ctcaatccat cacaggttct ggtcacgggt ttcgctgccg
     7201 tgataatcat cggggcaatt ctgctttcgc ttcctgcagc atcagccaac ggaaaaagca
     7261 tccggtttat tgacgccatt ttcaccgcta catccgccac ttgcgttaca ggcctcgtgg
     7321 tgctcgatac agccacatct ttttcgcttt ttgggcagat agtaatcatc atcctgatac
     7381 aaataggcgg attgggattc atggcgctgg ccacagcaat cgccttgttt ttgggccgaa
     7441 agattacgct gcgggggagg attcttctgc aagaatccct caaccagttt agcattgcag
     7501 ggctggtgcg cctcaccaga tacctcctct tgaccacatt catcgtggag ggtgtcgggg
     7561 cgatcattct gagccttagg ttttcgtcga tcatgcccac atcaaaggca atatactacg
     7621 gaatattcca ttcaatatcg gcgttctgca atgcaggttt tgatcttttt ggctctataa
     7681 ccgggccttt ctccagcctc acatactggg catcagatcc cacagtcgtt ctcgtcatag
     7741 gatcgttgat aatcattgga ggcctgggct ttccagtcat agtcgaactt gttggtcacc
     7801 gacggggcaa caggctgtcg ctccatgcca gactggtcct gacagtcaca ctctggctgc
     7861 tcgccatcgg cacggccgcc acattcgggt tggagttcag caaccccgac acactcggaa
     7921 aaatgtctcc ggcaggcaaa gtccttggcg cagtctttca aggcataacc ccgcggaccg
     7981 ctggctttaa cacccttgac atcagcaaga tgagaagctc gaccttgttc attttcgtaa
     8041 ttctcatgtt tataggagcc tctcccagtt ccaccggcgg aggcataaaa accacaacaa
     8101 tgggagtgat catcgcagcc atactctcca ctattcgtgg ttcagacgat gtggagatca
     8161 acgacaggca gatatcccaa agcctggtgt tcagggccat caccatcgcc agtctcgcac
     8221 tggccttggt aacaggcgtc actttggtac tctccgcaac agaagaggct tcgttccttc
     8281 aggtgttgtt cgaagccgcg tcggccttcg gcacagtcgg tctgacaacg ggcataacgc
     8341 ctaaccttac tgacttcggc agagtgctta taatgataac catgttttct gggcgtctcg
     8401 gccccctcac tgtgatgtta gctttggctc aacggcaaaa gcccttggcc aatgtccatc
     8461 tcccagaaga gaaggtgtta atcggatgag aatatatctt gttcgtcacg ggcaaaccgt
     8521 ccgcgggcgc gaagagacct tccgtggacg catggatgcg cccctcgatg acatgggcag
     8581 aaaacaagct gcggcggtcg ctacggcgct tcgcgatgtc aaactcgttg cggtctatac
     8641 gagccctctg tcgcgttccg tcgacactgc aagggcgata gctgtctcac atggcataaa
     8701 catcatcgtt cgcgacgcgc tcacagacat agatgtcggg gaatgggaag ggctttccac
     8761 cgaccggata aggtcggact acggaagact ctatctggac tggcaaacag gccccgattc
     8821 atttaccttc cccggtggcg agtctctgtc agacgtatct gaccgtgcca tggctgcaat
     8881 gggcaaaatc gtctcatcac acgagcatga cgatgagttg gcaatcgtct cccaccgcgt
     8941 gctgactaag ctcattttgc tcaatatgtt gcatgcccct gtatcgagtt tttggaagct
     9001 gtctcaggat ccatgttgca taaacgtggt ggattacacc gaggcaggtt atgttgtgga
     9061 atccgtaaat tgcatttccc acctggcatg agctgtgctg ccgaagtcgt agatcaacca
     9121 cacaacaacc tgttcattat gggcgacatc gctgctgtgc acctgcctga ttgtgtgagt
     9181 aatctcgcgg agcattgtcg ccacgacatt taatatggtg gcgccatgcg atcagcccgg
     9241 cgccatctcc tcaccgaacc gtacgtacct tctatggtgc tcaaagcaat ctcgaactag
     9301 gctttgccct atatacacat tcctacaggg gcaactttcc ccaggcggtg cccatgcgat
     9361 agccgtttga acgcgctcaa ccactttttc aaagctgctt ggactttttt cattccatag
     9421 gcccaacaaa catgccgcgt aaatgccgga aagcctgcct taaatggcaa gctttccgac
     9481 cctctgtcgg catcgttacc cacgcgcccg actcttactt gcagtttgcc acgagtccgc
     9541 caaggactgg acctggccac acgtttacgc tgccggttga gaaaaaggcc gcaggcaact
     9601 gctgaactgc agtgccagcc cagatcccgg tgcccagcgc ctctgtcata ggcacctgga
     9661 atgaataccc accccaagta aacagggccg tgtaagtagc cgctccaagg cttgtaggtg
     9721 acggcacatt agtgatgata accagaagct cagtcctacc tgatgcatca gttctcatcc
     9781 acagggcgcc tcctgccgtt gttacagggc ctgtcacaga cggagcgagg gcgagcgtac
     9841 acgttctggg cgccacagca ggcagaggca cgcatattac ctggcctacc tggagcctca
     9901 tcggatcgag gccggggttg gccgccagta ttgcctggac tgtcgttccg tacgcagctg
     9961 cgatcgtgta tagcgtgtct cctgctctga tagtgtagaa gactcctccc ggacaaaccg
    10021 tcggaggagg aacgggaatc ggcacgggaa tacatataac ctgcccaatc tggaggttca
    10081 tgggatcgat tcctggattt gcgcggagca acgcatcaac ggtggtccca tacctctgag
    10141 ctatcctgta tagagtatcg ccttgccgta tgacataagg cgtcccacca ggacagagaa
    10201 ccggaggcac ggggggcgtc ggcggcgtcg ggacagctac gggtatacag ataagctgcc
    10261 cgatctgcag ccttcgcggg tctacgccgg gattagctgc cgttatagcc tctacagttg
    10321 tcccgtatcg tcgggccaga gcgtagaatg tgtcgccagc cctgatctca tatagaaatc
    10381 cgcccggaca agccggcgtc tggcgcgttt cgacctcttc cctttcttca tctgtcattg
    10441 cccatcactc tcctggttag agtctagtgc accatatgcg cacggacatg ggtttgacca
    10501 tggacccaaa gcacatgtgc cagggtccga taggttcgta aataccgggc agcacccatc
    10561 ggcctatggg caatacgcta acctcccagt gcaatagccc aaagcccatc ctgttttatc
    10621 aatgcaggat ttgtgattct tccggagaat ttatcaagtg ttccccaaac actgcactac
    10681 aggtatagat aggcagcttc catggcataa acatacgact cagtcagcat catggggagc
    10741 atgcttgtca ttaaaaatgg aggggggaaa ggcgcctctt caggcgcaac gagtgcttgg
    10801 agtgcgcagc cgcgaagatg aaaagatctc ttgcaattgc catgtgcatc tcaatgctgc
    10861 taggattggc tacgtgcgcg tcggcatctc aagaaggcaa acttctcata tgggcagatg
    10921 gaacgcgagc ccctgtcatg gaggacctag gtaaaaagtt tacagccaag tacaaagtgc
    10981 ctgtggaagt tcaagaactg ggctttggtg acatccgcga ccagcttggc gtggccggac
    11041 cggcaggcaa aggccccgat atcatcatag gtgcccatga ctggctggga gagctcgtaa
    11101 ccaacggcct tatcgagccc atcgacctgc aggccaaaga gaaagatttc ctcaaagtct
    11161 cggttgaagc cttcacatgg ggcagtacat tgtacggagt tccttacgct gtggaggcag
    11221 tggggctctt ctacaacaca aagctcgttc ccaatccgcc aaagacctgg gacgacttgg
    11281 tggcaatctg caaaaagctt accgacaaag ataagaagca atacggattc cttcttccca
    11341 tacccgaccc ataccacaca ttcccgatca tgagtgccgc cggcggatac gtatttgggg
    11401 tgaacaagga tggcaccctc aacccgctcg acgtggggct caataatgcg ggcgcagtca
    11461 gaggcctcag cctcctcgac aagcttgttg aggacggact gatgccgcaa agtatggatt
    11521 acaacacaat gatgggcctc ttcaaagatg gcaaagtagg catgatgctc acagggccat
    11581 gggccctggc cgaccttcgc gcagcaaagg tcccttacgg gttcaccacc ataccaaaga
    11641 tagacggaaa ggatccccgc ccgtttgtcg gagttcaagg cttcatgatc agctcgttca
    11701 gcaagaacaa gatgctggcc aaagccttcc tcaacgaata cgtggctact gccgacacta
    11761 tgatggccct gttcaagcag gatccccgtc cgccggcgta tcttgcgacg gcaagagccg
    11821 tgtcgtctga cgcagacata agggctacgg gcgatagcgc agccgctggc atacctatgc
    11881 cggcaatacc ggaaatgtcc gcagtgtgga cggcatgggc caacgccata gaactcacgt
    11941 tcaaccagaa gctcgaaccg aagaaagctc ttgatgacgc aacggcacag atcatctctg
    12001 ccatcaaggc tagcaagaaa taggtttgca caatgttcac gcgatgcctg atttcgtggg
    12061 ggcgccgccc gtcatgccga cagttcgaca agggcgcgcg cccttgcgct agctcaggga
    12121 gggtgaaatg acttgaaagt cgcgacaaga cgcgcgcccg tcgatatgcc gattccagga
    12181 ttgattgcaa aggtcgcact cctgggaata ctcaacgcat tggccatctg ggccatattc
    12241 gttctgatag gcgaaagcgc ctattggata gctgtcatgc ttgcagcggg atgcgctctg
    12301 atcgattggg ttttcctgac tccgcgcctg ttcccatggc gctacttgat tccaggcctc
    12361 atcttcatgg cagccatggt ggtgtatccc atatgttaca cagtggctgt ggccttcaca
    12421 aactacggca ccggccacct cctcacaaaa gaacaggtaa tcgagaacta cctctcccgg
    12481 gccaccctgc aggaagatgc gccggagtac cctgctgcaa tgtacaaaag ccatgacggc
    12541 agattcgcag tcgtcctcat cgaccctaag ggagagaagt tgctcggcgt agacggtgaa
    12601 gttctcccgc ttggcgaggt ccaactgccc gttgaatacg cggattcaga tggcgttccg
    12661 gaaagcatag gcccctatca gcgcctcggc acgctcgaac tcttccagca catgagtgca
    12721 gtagaggctc tccagttcca cttcgaggac tttatactca agatgaggaa cgtggatcgg
    12781 tttgccacat ataagcctca atattcctac gatagcaatc tggatgccct cattgacacg
    12841 accaccggaa aagtttatac agcgacgcaa ggcacttttc tggcagacga cggggaagct
    12901 ttggacgtag gctatcgtgc gccggtcggg tggagcaatt ttgcgaggtt aatccgaagt
    12961 ccccaggtgt caggcccgtt tttcagggtg ttcgtatgga cttttgaatg ggcggcgctt
    13021 tctgtggtaa ccacgtttat cctcggcctt ctcctcgctg tccttctcaa tgaccagaaa
    13081 atgaagttca aaggattcta cagagttctg ctggttatcc cctatgcgat gcccgccttc
    13141 atatccgcgc tcatctggag aggcctcctc aacgcggaag tggggctcat caatgacatg
    13201 ctccagttcc ttttcaacgt gagagtgccg tggctgcagg atccattctg ggccaaggtc
    13261 gcactgataa tagtcaacct ttggcttgga ttcccgtaca tgatgctagt cagcctcggt
    13321 tcacttcaga gcatcccgga cgagctctat gaggcttccc gggtagacgg cgccaccggc
    13381 tggcagcagt tctggcatgt cacattcccg ctcctgctcg taagcgtagc gcccttgctt
    13441 gtgtcgagtt tcgctttcaa tttcaacaat tttggtgtga tatacctggt cacccaggga
    13501 agaccggcta ttcccggcgc gcagactcct gcaggggcaa ccgacatact gattacttac
    13561 acttaccatc tggcttttga aagcggttct ggaaccgact acgggcttgc ttcagcagta
    13621 accatattga tcttcctaat aatcggcaca ctgagctggt tcaacttccg cttcacaggc
    13681 gccctcgagg aggtgcacga gaatgtataa ggggttctta gaaccggcct acttgtggcg
    13741 ccacatagtc atatggctgt gcatagcatt cgccattctg ccggtgcttt ggataatatc
    13801 ggcttcgttc gacccttcaa acaccataat cgggcagaag cttataccga gcaatccatc
    13861 actggagaac tatagaaagc tatttaatga ccccactcac ccgttcggac ggtggatgct
    13921 aaacacttta aaggtctcat ttatcacgtc ggccctggtg gtcatcatga cgtcgttggc
    13981 cgcctattcc ttctcccgat ttcgtttccg cgggagaaag accgggctct tctcgatcct
    14041 gctgattcag atgtttccgc agatgctagc gatggtcgct atctacttac tcctgctctg
    14101 gatcggcgag cattttccga cgttcggcct caatacccat ggagggctca ttctggtgta
    14161 tttgggcggc gccatggggg ttaacgtgtg gctcatgaaa ggctacttcg acaccatacc
    14221 cacgtcgctg gaggaagcgg cgacaataga cggggcaaca cggttccaat gtttctacaa
    14281 ggtaatactg ccgctgtccc gccctattct tgtggttatc ttcttcctcc agttcattgg
    14341 gacctattca gagtatgtcc tggcaaaggt cctgttgtcg tcaaacgacg tccttacgct
    14401 ggcagtagga ctccaatcgt tcattggcga tcagtatgca aaaaggtggg gggtgttttc
    14461 cgctgccgca atcataggtt ctgctccaat ccttacgctc tttgcgcttc tccaaaacga
    14521 gctggtatcg gggctgacgc gcggcgcggt caaggaataa tcaattgcaa acaccaagaa
    14581 acgagggata ctaaaggttg cagaaacacg caatatggca tgtgccttac gatgcaatgg
    14641 catatccaat cagtgatgac cggcttgaaa tcgtgctccg tgcagccaga ggcgatctgc
    14701 ggcgagttga ggcagtatac accgacagat acacgccaca tgcccgcact agcagtactc
    14761 gtctctggat cgcaggatct gatggactct tcgattactg gagaggaagc atgaagcttc
    14821 aatatgggcg cttcatgtac tatttcgagc ttcaagatga tgaaaaacgc atctgctacg
    14881 gtgaaggcgg tttttcggac gaaccgccac aggacgagcc atggacatgg tgtttccaat
    14941 acccgtgcat ctggtatcat ggaggcatgg atgatccccc gaagtgggct caggacgcag
    15001 tggtgtatca gatctttgtg gatagattcg ccaatagcga ccgggcaaat gaccctcccg
    15061 gagtatccag atggggaacg ctgcccacac cgcgctcctt ttccggggga gacctggctg
    15121 gaatcatcga aaagctcgac tacatatcct ctttgggggt gtcgtgcctc tacctcaccc
    15181 ctatcttcct ttcgtcgtcg aatcacaagt acgacaccat tgactattat cacatcgatc
    15241 cgcatttcgg aaacgaggat acggcgcgac atcttgtgga tcagtgccac gcccggggaa
    15301 tccgcgtcgt tcttgatgct gtcttcaacc actccggcta cgactttttc gcatttcgcg
    15361 acgtggtaaa aaaaggcgac gcctcgccat actggaagtg gttcaatatc cacggctatc
    15421 cggtgcgcac acgtccaaag ccgaactacg aaacatttgg tcccgggata tggcagatgc
    15481 cgaaattccg gaccgacaat cccgaagtcc gcgattacct catctcagcg gctgagcact
    15541 ggacgcgcac actcgacatc gacggttggc ggcttgatgt ggcggacgat gtagaccagg
    15601 ggttctggcg cgagttcagg acgcggatgc ggcaaataaa gcccgacatc ctaatagtgg
    15661 gcgaaatcct ccatgatgcc tcgccatttc tccgcgggga ccagatggac tcggtcatga
    15721 actacccctg gagagagctg tgtctcgatt acttcgcccg ccgacgaatc gggactgccg
    15781 attttgcaga cggcataacc gccacagcca tgcactaccg cagacaaaca cagtatgcca
    15841 tgtggaacct tctggggtcc cacgatcgac agcggttcct caccgcatgc cggggagata
    15901 ctgcgaggct gatgctggct accacgttcc agttcacgta tacaggaact ccctacatct
    15961 actatgggga tgaaatcggg ctcaaaggcg gagaggatcc ggactgcaga aaatgcatgc
    16021 cttgggagga agacttatgg gacctcgatc tcctcgagca tcatcgggcc ctagcccggc
    16081 tccgagccga acatccggtc ctcaggcgtg gagacttcgc atcactcatt gcagacgggc
    16141 cggacagtcc ttacgtctat gctagatggg atgatgacgc gtgcatcgtc gtcgcgctca
    16201 acaacacaga agagcctcag acggtaaata cgaagcttgt cgcagagcgc atgagaaaga
    16261 cacacgaact agagcagtgg gccggcatgc cgatgaagca aatcttcagc ctgcacggcg
    16321 acaatactgg tcaaggcaga tccgacaata cgcaggcatc gatgtcggcg gtaggcgaga
    16381 attgtggccg gcatgtggtt ggcttgccgg gtatgggggc agccgttttt caagttcaat
    16441 cttagtcagg gaaagcatag caaaaactgc cc
//
LOCUS       DAOSAI010000011        15922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_2525_length_15922_cov_3.647949, whole genome shotgun sequence.
ACCESSION   DAOSAI010000011 DAOSAI010000000
VERSION     DAOSAI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15922
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2525_length_15922_cov_3.647949"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..2790
                     /locus_tag="PLM83_01015"
     CDS             130..2790
                     /locus_tag="PLM83_01015"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOK70024.1"
                     /translation="MGTTEISTTYNPKEIEEKTYQFWLERGYFHAEPDPDPEREHFVI
                     LMPPPNITGTLHIGHALDVTIPDILIRWKRMQGANVCWIPGTDHASIATEAKVVASLA
                     KEGLSKTDIGRERFLEIAWEWKEKYGGEIVEQQKKLGASCDWDRKRFTMDPVCSKAVR
                     EVFVRLFEKGLIYRGDRMINWCPNCRTSLSDVEVEHEDVESALWHLRYPLADGSGHVV
                     VATTRPETMLGDTAVAVNPKDPRYAKLVGKTLILPIMDREIPIVADAHVDPEFGTGAV
                     KVTPAHDPDDFEIGERHGLASVKVIGPDGIMTEEAGKYAGMTREECRRQVVEELKSAG
                     LLEKIESYEHAVGHCQRCGTAVEPMVSRQWFVKMKPLAEPAIEAAKDGRVRFVPERFT
                     KTYLNWMENVRDWCISRQLWWGHRIPVWYCTSCDHMFASREDATSCPKCGGQVEQDPD
                     VLDTWFSSALWSFSTMGWPEETPELKSWHPTSVLVTGYDIIFFWVARMIFMSLEFMGE
                     EPFSDVLLHGLIRNPDGSKMSRSKGTGVNPLEIIEEYGADTLRFTLITGNTPGNDIRW
                     RPEKVEASRNFCNKIWNASRFVMMNLEDFTLPEGKVMPEDLELNLADRWIISRMNRVA
                     ADITEQLGRYDMGEAARTIYDFVWGEYCDWYIEIAKRRLYGEDDPVGRRTAQYVLWKV
                     LDGSLKMLHPFMPFITEGIWQHLPGSGESIMIEPWPTADKDEIDAVAEAEMAVLMETV
                     RAIRNIRAEFNVEPGKKVDAIFHADGEAFDTINVNVDIVAHLAGLASVTVEHVSAAKP
                     EKAAAAVVPGIEVYLPLAGMVDIGKETNRLRREEENLLREVKSARAKLSNEGFVTKAP
                     AEVVQKQRDRLGEYEEQLVKIRARIAQLEG"
     gene            2794..3603
                     /gene="nth"
                     /locus_tag="PLM83_01020"
     CDS             2794..3603
                     /gene="nth"
                     /locus_tag="PLM83_01020"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOK70025.1"
                     /translation="MMSDRHTDEVGVPMDVAGGEGWVRAPSSVSLEIVRRVDEALRHM
                     YGPKMLAPNGDPVGELIHIMLTQNTSDANSDRTYAALRSAYSSWEAVAAASPSEIAEV
                     IRMGGLADIKAARIIEVLSRIEADYGCISLDAIDSMNDDEAFDYLTSLPGVGPKTAAC
                     VLLFALGRPVFPVDTHVHRVANRIGLVDTKQPAKTQEELGAVVPEDIVYQLHMNMVAH
                     GRRTCRARRPKCEECALRDECAWAIAASCKAGDKGVSEESVAERVAEGGRA"
     gene            3670..4983
                     /locus_tag="PLM83_01025"
     CDS             3670..4983
                     /locus_tag="PLM83_01025"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOK70026.1"
                     /translation="MDYEAAVDYLRSLSRFGIKPGLERTEALLQSMGNPERSMAHVHI
                     AGTNGKGSVSVMVESALEEAGHVSGLLTSPHLQRYTERIKVGGSEISQQQLAELVGEM
                     APVIAKLSENENIGTPTEFEALTAACFEWFMRSGVEIAVVETGLGGTYDSTNVVTPEV
                     SVITHIAMDHMDRLGNTLSEIASNKAGIIKPGVPVVVSPQDPEAMAVIVAKANEVGAE
                     VTVVGMDVPYEPIHMDLDGTTVDVDAPVLGSLRVHTPLVGRHQVTNCATAVAALDVLE
                     GLKGGTISPAAVCSGIGKACHPGRFEVVRKSPYVVILDGAHNLDGAASLAATLHEIAY
                     GLRPRVMVFGTSRDKPVREMLAALVPEVDVVIATAAVGARTGAHDPEFVASVAREMGV
                     EAHAVASACEAVQAGARTASAIGAKSLLVAGSLYLVGEVRGIWRD"
     gene            4986..5720
                     /locus_tag="PLM83_01030"
     CDS             4986..5720
                     /locus_tag="PLM83_01030"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HOK70027.1"
                     /translation="MTQGWAEMEKFRKARVTVLVGAFGSGKTELALNFAKRLNAAGRN
                     AVLVDIDILKPMFRSRDKRNDLSELGVRVVSSVTDYEDSDVPALSPAIYGVIEDRSSD
                     VVVDVGGDDDGARALGRFREALEEAGYEMLYVVNTRRPFSSTPEEIVSMVLAVEEASR
                     LRCTALVSNTNLGSESFVGLVEEGLDITLQASQTLGVPVRFAMIQKSVADSQRNELEK
                     IVQVYGVPVFEMDRSILPPWEGIAQS"
     gene            5805..6404
                     /locus_tag="PLM83_01035"
     CDS             5805..6404
                     /locus_tag="PLM83_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOK70028.1"
                     /translation="MPAKRSSSGNALSWILAVVLISVAAVGVGWLIGQQVLGWINPNR
                     SSVSEEDSGDTGGYSYYPWSGSDGAGSASQQPQQTPAPNINLPTSSLPESSAVEPPTY
                     GAESSSVPSSVPDGIGSTQGTVSSPVAAEFYKVRVGSFGTKEAAAATARELEAQGYPI
                     FVTGGGPWSVQVGAFSLRENAIVLGDILANQGYDVTIVE"
     gene            6646..7029
                     /locus_tag="PLM83_01040"
     CDS             6646..7029
                     /locus_tag="PLM83_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOK70029.1"
                     /translation="MKLADIRELIGAEVLVGEDKLSTVEIEMACGSDLISDVLAFCHD
                     KTLLCTGLVSAQVVRTAEIAGLSTIIFVRGKRPDESVIEMGKAAGIPMLATDLPMFET
                     CGLLYGAGVGGICLGADGSRRGDVG"
     gene            7122..7529
                     /locus_tag="PLM83_01045"
     CDS             7122..7529
                     /locus_tag="PLM83_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK70030.1"
                     /translation="MSFRVDRYDFAAAGEAASKIKQVLRQLGMAPELVRRAAVAAYEA
                     EMNLVIHSEGGQILLRVDTEKVTLVACDGGPGMPDVSLAMREGYSTAPDEIREMGFGA
                     GMGLPNMKRCSDSFSIESEVGRGTTVTAAIRIA"
     gene            7582..8922
                     /locus_tag="PLM83_01050"
     CDS             7582..8922
                     /locus_tag="PLM83_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOK70031.1"
                     /translation="MGMEGRFHSVKLLEEKCKGCTNCIKRCPTEAIRVREGKAVINAA
                     RCIDCGECVRTCENHAKTIIANSIDDLKNYDYTIALPAPALYSQLGRASGPDEVVWAL
                     QEIGFDAVCDVSFGADIVTECTRRHLISHPKPRPVISAACPAVVRLIQVRYPNLISHV
                     ARVESPMYAAARVARVEAAEKLGIAGDRIGVFFISPCAAKVTAVIAPLGSEFSYVDGA
                     ISISDIYGELMTELGRGRSARPMHRGSNLGFAWANSSGELKALGLHNVLAVDGIHNVL
                     GLLEEVDNGRLAGVDYIEALACPAGCVGGPATVENPFVARARMIEISKAVEADSAEAL
                     SEARRLVDERGTDFFALQGELTALSGLEIDPRLEVAIAKMSELERVLARLPGLDCGAC
                     GSPTCRAHAEDVVCGNASEVDCVFKLREAMEEVAEELLRLAKTDLPSLQRRNDK"
     gene            8919..9263
                     /locus_tag="PLM83_01055"
     CDS             8919..9263
                     /locus_tag="PLM83_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOK70032.1"
                     /translation="MTVREIAAVLGMKCVAGGVGLDREVSSGYVSDLLSDVMANASDG
                     GIWVTSQVHQNVVAVALLLDLAAVVIAGGLEMNEDAIARAEAEGMPILSTKMSAFETV
                     GRLYELGVRGDS"
     gene            9266..10009
                     /locus_tag="PLM83_01060"
     CDS             9266..10009
                     /locus_tag="PLM83_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOK70033.1"
                     /translation="MVQGFAADLHIHTFLSPCAGREMNPKAIVGRCLDLGLDIIASAD
                     HNSAANVEALAAAAEGTGLTVICGMEVCTKEEVHLLTLLPDLSAMHEWELIVAPHRAT
                     GRNIPEIFGRQWICSPRTGDITEETALLSAPTTLSVDEVVDGVDALGGISVPCHIDRR
                     AFSIVAQLGFVPDHLDIPVLEISRTITATEARAKFPATQGRVLLSSSDAHTLEEIGLG
                     CSLFWLEEPTFAELILALRGEAGRYVQVE"
     gene            10086..10613
                     /locus_tag="PLM83_01065"
     CDS             10086..10613
                     /locus_tag="PLM83_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK70034.1"
                     /translation="MHILDIAQNSIAAGAELVEIDVIEDVANDKMIIRVTDDGRGMPA
                     DVVAQIRDPFVTSRTTRRVGLGIPLLAAAAERCGGGVDIASVPGGGTTVTAILEYSHV
                     DRAPLGNMAETLLALVVCNPNLDFVYNHVRGDNSFHFDTREIRLELGSIPLSDPEVVA
                     FIRDFVAEGERRLDA"
     gene            10610..11098
                     /locus_tag="PLM83_01070"
     CDS             10610..11098
                     /locus_tag="PLM83_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOK70035.1"
                     /translation="MRQFGIVKRVCGESVEVLVSRPAACKGCGACQMANNRPHTVIAR
                     NDASAEVGDTVALELPGQDLMRAVLLAYGVPLVMFLAGLGVGALAARSFGWTIDPSLA
                     SAIGGILFLAAGYYGIHLYDRSLGTEAFASVAVAVVDSNNPECASIHSGAGRVEDTEE
                     TE"
     gene            11095..11760
                     /locus_tag="PLM83_01075"
     CDS             11095..11760
                     /locus_tag="PLM83_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010273782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOK70036.1"
                     /translation="MKQEKIPDVVVRRLPLYLRAVEDFDRREHAIVSSSQLGEWTGLT
                     PAQVRKDLAFFGEFGKQGIGYNVKFLHEQLREILGLTTDVRVGLVGLGNLGRALLHHN
                     VDARIDGSAVEGDPARWVPESLRIVAAFDVDPQKVGITYAQVPVYHVDELAAKVRELD
                     LKIVVIAVTPESAGAVASECVKAGVKAILNFAPVKLDVPEDVKVHSADLTLELQSLAF
                     YTR"
     gene            12310..13653
                     /locus_tag="PLM83_01080"
     CDS             12310..13653
                     /locus_tag="PLM83_01080"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOK70037.1"
                     /translation="MELNQELMIGSVYARDAIHSLYGGQRGLRISTPKAAPVVLLFAR
                     KSCDFQVRHGVDCGSPEGEHSCALPEDELPIAHTASAELAPYVSDPEEGWRDDGFYHF
                     RGEKHVQFLRGNRAVRDHARDGKLLFLFEETTRPEGRQAWRFIGPMECAGYYFAQASA
                     GRMAIMFRLAPVYAIDRLRKNSQELVLHSVLAERCALDFLNSRHGAIAASAESTRPGH
                     HSNAWKWSSDCALIQRYALDRAFGFCESCGKPSPFSDPDGEPYLEPHYMLRPYDSGVD
                     PVHGIAACCPECHRMIHDAWNGRSHDVALARKIALLERSVDRGDFLAVTAAVIRNAEG
                     KVLLTQRGHGALAGKWEFPGGKIEPGETLEACLAREIREELNLDIADIRPLVSSFYQY
                     DDSAVRLFGLSANAVGNQLELREHISKAWVWPHELESYDLASADLPIAAVAARLL"
     gene            13865..14716
                     /locus_tag="PLM83_01085"
     CDS             13865..14716
                     /locus_tag="PLM83_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOK70038.1"
                     /translation="MFKIVEKCTLAPGVHQYEVEAPLVAKGARPGNFVVVRTSETGER
                     IPLTIAGHNRETGTITLVVQEVGKSTMEMGMLEVGDYFKDVAGPLGQATRIEKVGTVV
                     AVAGGIGVAPVLPQIRAHKAIGNHVISIVGARTRDLLVLRDEVEKFSDEVIWATDDGS
                     LGYHGFVSGALEELIGSGQKIDEVIAIGPVRMMQAVADVTRKHNIPTTVSLNAIMVDG
                     TGMCGCCRVTVDGEVKFSCVDGPEFDGHKVDFDELVSRQGFFRDKEQIARERFEADRG
                     GCKCHAK"
     gene            14731..>15922
                     /locus_tag="PLM83_01090"
     CDS             14731..>15922
                     /locus_tag="PLM83_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK70039.1"
                     /translation="MPKQSPEARRTNFSEVALGLDPELARREAERCIQCKTRPCTTGC
                     PVEVDIPEFVALIAEGKYDEAAAKIKEKNSLPAICGRVCPQESQCESLCTLGRKYEPV
                     AIGALERFAADWASKRAVEKREACAGDVDSDLQCGCEQKVCELDSRGCAPTSDSAASA
                     ARVAVIGTGPAGLTCAADLALKGYKVDMFESLHDTGGVLRYGIPEFRLPKSIVDEEVE
                     YVRSLGVRIFTNVVVGLTLDLREMLESGQYQAVFIATGAGLPYFLGIPGENLNGVYSA
                     NEFLTRVNLMKAYMFPQYDTPVNIGSKVAVVGAGNVAMDSARTSLRLGAEEVKIVYRR
                     SRAEMPARLEEIENAEEEGVEMMLLANPVRIISDGCGKVSGMECIRMELGEPDASGRR
                     RPVPV"
BASE COUNT         3543 a         3811 c         5069 g         3499 t
ORIGIN      
        1 aaggtggtac cgcgaaggaa cgcctccttc gccctttggt gggcggggag gcgtctgttt
       61 ttcaaaatgt gtcggacaca tcctgcgacg cctgcaggca tgtccgcggc tacaggagga
      121 aagaagctca tgggcacaac agagatatcg actacttaca acccgaaaga gattgaggaa
      181 aagacttacc agttctggct cgagcgtggc tatttccatg cagagcccga cccggatcct
      241 gagagggagc atttcgttat cttaatgccc ccgcccaata tcacggggac gctccacata
      301 gggcatgccc tcgacgtcac gatcccggac attctgatca gatggaagcg gatgcagggg
      361 gccaacgtct gctggatacc gggcacagac catgctagca tcgccactga ggccaaggtc
      421 gtggcgagcc ttgcaaagga aggcctctcc aagacggaca taggccgtga gcgctttctc
      481 gagatcgcgt gggagtggaa ggagaagtat ggtggagaga tagtggagca gcagaagaag
      541 ctgggcgcgt cgtgcgattg ggacaggaaa cgtttcacca tggaccctgt atgctccaag
      601 gctgtgcgcg aggtattcgt gaggcttttc gagaaggggc tcatctaccg gggcgatcgg
      661 atgatcaact ggtgtccgaa ttgcagaacc tcgttatcgg atgtagaggt ggagcacgaa
      721 gacgttgaat cggctctgtg gcatctgcgc tatccgctgg ctgatggatc cgggcacgtg
      781 gtagttgcca cgacgcgccc tgaaactatg ctgggcgata ccgcggtcgc tgtgaacccg
      841 aaggacccga ggtacgccaa gctcgtcggg aaaaccctca tcttgcccat tatggacagg
      901 gagatcccga ttgttgccga tgcccacgta gacccggagt ttggaaccgg ggccgtgaaa
      961 gtcactccag cgcatgaccc tgatgacttt gaaatcggcg aaaggcacgg actagcctcg
     1021 gtgaaggtca taggccctga tggtatcatg actgaggagg cagggaagta tgccggcatg
     1081 acccgggaag agtgcaggcg ccaagtggtg gaggaattga agagcgcggg cctgctggag
     1141 aagatcgaaa gctacgagca tgcggtcggc cattgccagc ggtgcggcac tgcggtagaa
     1201 cccatggtgt cgaggcagtg gtttgtgaag atgaaacccc tggccgaacc ggcaattgag
     1261 gcggcgaaag acggaagagt gagattcgtt ccggaaaggt tcaccaaaac ctatcttaac
     1321 tggatggaga acgtgcgcga ttggtgtata tcccgtcagc tttggtgggg ccatcgaata
     1381 cccgtctggt actgcaccag ctgcgaccac atgttcgcct ccagggaaga cgccacttca
     1441 tgcccgaaat gcggcggaca agtggagcag gaccctgatg tgctcgacac ctggttcagc
     1501 tcggcgctgt ggtctttttc gaccatggga tggcctgaag aaactccgga gcttaaaagc
     1561 tggcatccaa cctcagtgtt ggtgacaggg tacgacatca tcttcttctg ggttgctagg
     1621 atgatcttta tgagcctgga gttcatgggc gaggagccat tttccgatgt gctcttgcac
     1681 gggctgatca ggaatcccga tggttccaag atgagcaggt cgaaggggac aggtgtcaac
     1741 ccgctcgaga tcatcgaaga gtatggcgcc gacacgctca gatttactct gatcacaggg
     1801 aatacccctg gaaacgatat taggtggcgt ccggagaaag tggaggcttc ccgcaatttc
     1861 tgcaacaaga tatggaatgc gagcaggttc gtgatgatga acctcgagga cttcactttg
     1921 cccgagggca aagtgatgcc ggaagatctg gagttgaacc ttgctgacag gtggatcata
     1981 tcgcgcatga acagagtggc tgccgacatc actgagcaac tcggaagata cgacatgggc
     2041 gaagcggcgc ggactatata cgatttcgta tggggcgagt attgcgactg gtatatagag
     2101 atagccaagc gccgcctgta cggagaggac gatccagtcg gcaggcgtac agcccagtac
     2161 gttttgtgga aggtgttgga cggttcactc aagatgctcc atccctttat gccattcatc
     2221 accgagggca tttggcagca tttgcccggc tcaggcgaga gcataatgat agagccctgg
     2281 cctacagcgg ataaagacga gatcgacgct gtggccgaag ccgagatggc cgttttgatg
     2341 gagacggtga gagccatcag gaacatcagg gctgagttca acgttgaacc aggcaagaag
     2401 gtggacgcaa tattccacgc agacggtgag gcgttcgaca ctatcaacgt caatgtggac
     2461 atcgttgccc accttgcggg acttgccagc gtgactgtgg agcatgtgtc tgcggctaaa
     2521 ccagagaaag ctgctgcagc ggttgtgcca gggatagaag tctatcttcc ccttgcgggg
     2581 atggtcgaca tcggcaagga gaccaacagg ttgcgccgcg aggaggagaa cctcctgcgc
     2641 gaggtgaaat ccgcccgcgc gaagctctcc aatgaaggct tcgttacaaa ggctcctgcc
     2701 gaagtagttc agaagcaacg cgacagactc ggggagtatg aggagcagtt ggtgaagatc
     2761 agagctagaa tagctcagct agagggataa gtgatgatgt ctgacaggca cactgacgag
     2821 gtcggggtcc ccatggacgt tgccgggggc gaaggctggg tccgtgcccc ctccagcgtc
     2881 tctttggaga ttgtgaggcg cgtggacgaa gctcttagac acatgtatgg accgaagatg
     2941 ctggcgccaa atggcgatcc cgtcggcgag ctgatccaca tcatgcttac gcagaatact
     3001 tcggacgcga attccgaccg gacttacgct gcattgcgaa gcgcctattc ctcatgggag
     3061 gctgtggctg ccgcatcccc gtccgagatc gccgaagtga tccgcatggg cgggcttgcg
     3121 gacataaagg ccgcgcgcat aatcgaagtc ctttccagga tagaggcgga ctacggctgc
     3181 attagcttgg atgccatcga ttccatgaat gatgacgagg ctttcgatta ccttacttcg
     3241 cttccgggtg tagggccgaa gaccgccgcg tgcgtactct tatttgcctt ggggaggcca
     3301 gtctttccag tagacaccca cgtgcacagg gttgcaaacc gaattggcct tgttgatact
     3361 aaacagcctg ccaagacaca ggaagaactg ggcgccgttg tgccggagga catcgtctac
     3421 cagctgcaca tgaacatggt ggcccatgga aggcgaacat gccgcgctag gcgtccaaag
     3481 tgcgaagagt gcgccttgcg ggatgagtgc gcgtgggcga tagctgcaag ctgtaaagca
     3541 ggagataaag gcgtttcgga agaatcagtc gccgagcgtg tggccgaagg cggaagagct
     3601 tgaagagggt ttgctccaat gctcatgtat tcttcgagag ttgttttatg aatgtgaggc
     3661 atggagtaga tggattatga agcagccgtt gactatctga gatccctttc gagatttggg
     3721 atcaagccag gattggaacg cactgaggcc ttgcttcagt cgatggggaa cccggagcga
     3781 agcatggctc acgtgcacat agcgggaaca aacggcaagg gttctgtttc ggtgatggtc
     3841 gaatccgcgc tggaagaggc cggacatgtt tccggattgc tcacatcgcc tcacctgcag
     3901 cgctacacgg agcggataaa ggttgggggc agtgagattt cccagcagca gctcgctgaa
     3961 ttagttggag agatggctcc cgtcatcgcc aagctgagcg agaatgagaa catagggact
     4021 cctacggaat tcgaggcgct gactgcagct tgttttgagt ggttcatgcg ctccggtgtg
     4081 gagatagccg ttgttgaaac aggcctaggc ggtacctatg actcgacaaa cgtggtgacc
     4141 cctgaagtga gcgtgattac ccacatagca atggaccaca tggatcgact gggcaacacc
     4201 ctttctgaga tcgcttccaa caaggccggg atcatcaagc cgggggtgcc tgtagtcgtg
     4261 tcgccacagg atcccgaggc tatggccgtt attgtcgcca aggcgaacga agtgggggcc
     4321 gaggttacgg ttgtcggaat ggatgtccct tacgagccta tccatatgga tctagacggc
     4381 acgactgtgg atgtggatgc tcctgtcctc ggtagtttgc gtgttcacac tccccttgtc
     4441 ggacgtcatc aagtgactaa ctgtgcaacg gcagtagctg ctctagatgt tcttgaagga
     4501 ctgaaaggcg ggactatctc acctgccgcg gtttgcagcg gaatagggaa ggcgtgccat
     4561 cccggacgat tcgaagtcgt ccgcaagtcg ccatatgtcg tgattctgga tggtgctcat
     4621 aatctggatg gggcggccag ccttgcggcg acgcttcacg agattgcgta tgggctgagg
     4681 ccgagggtga tggtgtttgg cacgtcccgc gacaagcctg tccgcgagat gctggcagca
     4741 cttgtgcccg aggtggatgt ggttatcgcc actgcggcgg ttggcgctag gacaggggca
     4801 catgatcctg agttcgtggc tagcgttgcc agggagatgg gggtcgaggc gcatgcagtc
     4861 gcttccgcct gcgaggctgt gcaggccgga gctcggactg catctgcgat tggggcaaag
     4921 tcattgctgg tggcagggtc gttatatctg gtgggcgaag taagggggat atggagggac
     4981 tagatttgac gcaggggtgg gcagagatgg agaagttccg aaaggcccgc gttactgtgc
     5041 tcgtgggggc ctttggatcg ggaaagaccg aactcgcgct caacttcgcg aagcggctga
     5101 acgccgcggg acgaaatgcg gttctcgttg atatcgatat tttaaagccg atgtttcgtt
     5161 ccagggacaa aagaaatgac ctatcagagc tgggagtgcg tgtggtatcg agcgtcactg
     5221 attatgagga ttccgatgtt cctgcacttt cgcctgctat atacggggtt atagaggaca
     5281 ggtcatcaga cgtcgtggtc gatgtgggcg gtgacgatga tggcgcgcgg gcgcttggga
     5341 ggtttcgcga ggcgctcgaa gaagctgggt atgaaatgtt gtatgtggtc aatacgcgca
     5401 ggcccttctc gtcaactccg gaggagatag tctccatggt gctggcggtt gaggaggcgt
     5461 ccagactccg atgcactgca ttggtgtcaa acactaatct gggttcggag agctttgttg
     5521 gactggtgga ggaaggactc gatattacgc tgcaggcatc acaaaccttg ggagtgccag
     5581 tgcggtttgc gatgatccaa aagagtgttg ctgattcgca gaggaatgag ttagagaaga
     5641 ttgtgcaggt ctacggggtg cctgtgttcg aaatggatcg atcaattctg cctccttggg
     5701 aaggaattgc acagtcatga gtggtattac acaagaatag aagtatctac tgaggcgggg
     5761 ccatgccccg taccggatga atcgagcaaa ggaagggata tcacatgccg gcgaagcgat
     5821 cgtcctcagg caatgctctg tcatggatcc ttgcagtggt cttgataagc gtggccgcgg
     5881 tcggggttgg ctggctcatc gggcagcaag tgctgggctg gattaacccg aaccgaagct
     5941 cagtatctga agaagactca ggagatactg gaggttacag ctattatcca tggagcgggt
     6001 cggatggagc aggaagtgcg tcccagcagc cacagcagac gcctgctccc aacatcaatc
     6061 tgcccacaag cagtctgccc gaaagcagcg cggttgagcc ccctacatac ggggcagaaa
     6121 gctcatcagt acccagttct gttccggatg gcattggttc gacacaaggc acggtttcca
     6181 gtcccgttgc tgctgagttt tacaaggtac gtgtgggttc tttcggcaca aaggaggctg
     6241 cggcggccac cgccagggag ctcgaagctc aaggttatcc gatattcgtc acaggtggtg
     6301 gtccatggtc tgtccaagtt ggagcgtttt cgcttaggga gaatgccatt gtgctaggcg
     6361 acatccttgc aaatcagggc tacgacgtta caatagttga atagctgtgc gcatgccatt
     6421 tccagggcta atgaaacagg cgttggcgcg tggagcgtcc gccatgggcg gcaacgatcg
     6481 gttatgcgcg gtagtttgcg gagcgatcag caataatgat acaggcccgg tccgggcgca
     6541 aaaagtgtgg gccgggccag gcgcttttcg gatgagtgtc gggtccacag tcttgaatac
     6601 gccgctcctt gagtaggagc gtagagtgaa acgagggagc gaggcttgaa gcttgcagac
     6661 atccgggagc ttataggggc ggaggtcctt gttggggagg acaagctttc cactgttgag
     6721 atcgaaatgg cgtgcggctc ggacctcatc agtgacgtgc tggcattttg ccatgacaag
     6781 acgcttcttt gcacgggact cgtcagcgcg caggttgttc gcacggcgga gatcgctggg
     6841 ttgtccacga taatattcgt tcgggggaaa aggcctgatg agagtgtcat agagatgggc
     6901 aaggctgccg gaatcccaat gcttgcaacg gatcttccga tgtttgaaac ttgtggcttg
     6961 ctatatggcg ctggcgttgg cggcatctgc cttggcgccg atgggagcag gcgaggagat
     7021 gtcggatagt gtatcgcggt ggcaatgatg gacaagccat tgatcgggct ggtgctgctt
     7081 tttccggggc agccagtcct cactcggaac cggtgctaga gatgagcttc cgagtcgacc
     7141 gatacgattt cgctgcagcc ggcgaggcgg ccagcaagat caaacaggtt ctgaggcaat
     7201 tgggcatggc acccgagttg gtacgccgtg cggcagtggc agcgtacgag gcagagatga
     7261 acctagtcat acactcggag ggcggacaga tccttctccg tgtggacacg gagaaggtga
     7321 cgcttgtcgc ttgcgatgga ggaccgggaa tgcccgatgt ttccttggca atgcgcgaag
     7381 gctactcaac agcgccggat gagataaggg agatggggtt tggggccgga atggggcttc
     7441 cgaacatgaa gaggtgttct gattcgtttt caatagaatc tgaggtggga cgcgggacga
     7501 ctgtgaccgc agcaatcagg atagcctaaa gaagcagagc agccaaaacc gtggcagttc
     7561 gcgttccatg gggaggttta aatgggcatg gagggccggt tccattctgt gaaactcctc
     7621 gaggaaaaat gcaaaggctg caccaactgc attaagcgtt gcccgactga ggcaatcaga
     7681 gttcgggaag gaaaagctgt aatcaacgct gcccgatgca tcgattgcgg agagtgcgtg
     7741 agaacctgcg aaaaccacgc caagaccatt atcgctaact ccatcgacga cctcaaaaac
     7801 tacgattata ctattgctct accggcgccg gctctgtact cgcaactagg ccgggcctca
     7861 ggccctgacg aggtggtatg ggctcttcag gagatcggct ttgacgcagt gtgcgacgta
     7921 tcctttggcg cagatatcgt taccgagtgt acgcgcagac acctaatatc ccatcctaag
     7981 ccccggccgg tgatttccgc ggcgtgtccg gccgtagtcc gcttgattca ggtgagatac
     8041 cccaacctca tttcccacgt agcccgagtt gagagcccta tgtacgctgc ggcccgtgta
     8101 gcccgagttg aagccgccga gaagctcggc atagcgggcg atcgcatcgg cgtgtttttc
     8161 atctctcctt gtgcggccaa ggtaacagcg gttattgccc cattgggatc agagttttcg
     8221 tacgtggacg gtgcgatttc gatatcagac atatacggcg aactcatgac tgagctagga
     8281 cgaggacgca gcgcccggcc catgcacaga gggtcgaacc ttgggtttgc atgggcaaac
     8341 agcagtggag aactgaaggc gttggggctt cataacgtct tggctgtgga cggaatacac
     8401 aatgttctgg gactccttga agaggtagac aacggcaggt tggcaggggt ggactacatc
     8461 gaagccttgg catgtcctgc ggggtgtgtt ggaggtccgg ctacagtcga gaaccccttc
     8521 gttgcgcggg cgcgtatgat cgagatctca aaagctgtgg aggctgattc cgctgaagct
     8581 ctctccgagg caaggcggct ggtagatgag cgcgggacag atttcttcgc tctccagggc
     8641 gagcttacgg ccctctcagg tcttgagatc gatccgcgcc tcgaagtagc cattgccaaa
     8701 atgagtgagc tggagcgagt tttggctcgg cttccgggct tagattgcgg tgcctgcggg
     8761 tcgccaactt gccgtgccca tgcagaagat gttgtgtgcg ggaatgcatc tgaagtagat
     8821 tgcgtgttca agctgagaga ggccatggaa gaggtggccg aggagctcct cagattggcc
     8881 aagacggatc ttccgtcgct gcagaggagg aacgacaagt gactgttcgg gagatagctg
     8941 ccgttttggg aatgaaatgc gtggcgggag gcgtagggct ggacagggag gtctcatccg
     9001 gatacgtttc agatttgctc agcgatgtca tggcgaatgc aagtgatggc gggatatggg
     9061 ttacttcaca agttcaccag aatgttgttg ctgttgcgct cctgctcgat ctcgcagctg
     9121 tcgtgatcgc aggggggctt gagatgaatg aggatgccat tgccagggca gaggcggaag
     9181 gcatgccaat cctgtccacc aagatgtcgg cgtttgagac agtcggcaga ctatacgagc
     9241 tgggcgtgag gggagattcc tagggatggt tcagggcttc gccgccgatc tgcacattca
     9301 cactttccta tcgccatgcg cgggcaggga gatgaacccg aaggcaatcg tggggcggtg
     9361 cttggaccta gggctcgaca taattgcctc tgcagatcac aactcagccg ccaacgttga
     9421 ggctttggct gcggcggcag aggggactgg acttaccgtc atctgtggca tggaggtttg
     9481 cacaaaggaa gaggttcatc tgttgacatt gctgccagat ttgtctgcca tgcatgaatg
     9541 ggagctgata gttgcacccc atagagctac cggcaggaac atcccggaaa tattcggaag
     9601 gcagtggatt tgttcgccgc gcactggaga tatcacagag gagaccgcgc tcttgtccgc
     9661 cccaacaacg ctatccgtgg acgaagtcgt ggatggcgtt gacgccttgg ggggcatttc
     9721 ggttccatgc catatcgaca ggcgtgcttt cagcatcgta gcccagctag gatttgtccc
     9781 ggaccacctc gacattcccg tactggagat atccaggacg attacggcaa ccgaggccag
     9841 agcgaagttt cctgcgacgc aaggccgcgt cctgctctct tcctccgacg cccacacttt
     9901 ggaagaaatc ggattgggat gctcgctctt ttggctggaa gaaccgacat ttgctgaatt
     9961 aattcttgct cttcgcggag aggcaggaag atacgtgcaa gtggagtaaa ggcgattgct
    10021 tatagcggat tttccgtgct gggcagagta tacagaaatg gcggtgtggt atgagggagc
    10081 tttcaatgca catactcgat atagcgcaaa actcgatagc tgccggcgca gagcttgttg
    10141 agatcgatgt tatcgaggat gtggccaacg acaagatgat tatcagggtt accgatgatg
    10201 gccggggaat gcctgccgac gtcgtggcgc agatccgcga tccatttgtt acatcgagga
    10261 ctacccgccg agttggactg gggattccgc tattggctgc agctgctgaa agatgcggtg
    10321 gcggagtgga catagcgagc gtgcctggcg gtggcacgac tgtaacagct attcttgagt
    10381 attctcacgt cgaccgggcg cccttgggca acatggcaga aacccttctt gcattggtag
    10441 tatgcaaccc aaacctggat ttcgtgtata atcatgttcg aggagataat tcatttcatt
    10501 ttgatacccg agagattcga ttggagttgg gcagcatccc tctgtcggac ccggaggtcg
    10561 tagcgtttat tcgtgatttc gtagcagagg gcgaaaggag gctggatgca tgaggcagtt
    10621 tggcattgtg aagcgggttt gcggagaatc cgtggaagtc ctggtcagcc gtccggcagc
    10681 ctgtaagggc tgcggagctt gtcagatggc gaataatcgt ccccataccg tgattgcgag
    10741 aaacgatgcc tcggcggaag taggcgacac agtggcattg gaacttcccg gacaagatct
    10801 catgcgtgca gtccttctcg cctatggagt gccattggtc atgttccttg ccgggctggg
    10861 agtgggtgcg cttgcagccc ggagcttcgg atggacgata gacccgtctc ttgcttccgc
    10921 tataggggga attctctttc tagctgccgg ctactacgga attcacctct acgatcgttc
    10981 tcttggaacg gaagcttttg catctgttgc cgtggctgtg gtggattcga acaaccctga
    11041 gtgtgcgtcg attcattcag gggctgggcg ggttgaggat acggaggaga ccgaatgaag
    11101 caggagaaga taccagatgt agttgtaagg aggcttccac tgtacctacg cgcagtggaa
    11161 gactttgatc gcagggaaca tgcaatcgtc tcatctagtc agctagggga gtggacaggg
    11221 ttgactccgg cgcaggttag aaaggacctt gcgtttttcg gagaatttgg caagcaaggc
    11281 attggttaca atgtaaagtt cttgcatgaa caactaaggg agatacttgg ccttaccaca
    11341 gatgttcgcg tgggcctggt tggcctggga aatctgggcc gcgccttgct ccatcataat
    11401 gtagatgctc gcatcgacgg ctcagccgta gaaggcgacc ctgcccgttg ggtccctgaa
    11461 agcctgcgta ttgtcgccgc tttcgatgtg gacccacaaa aagtgggtat tacctatgcc
    11521 caggtcccag tctaccacgt agatgagctt gctgcgaaag tccgcgagct cgacctgaag
    11581 attgtagtta ttgcagttac gcctgagagc gcgggagctg ttgctagcga atgcgtgaaa
    11641 gcgggggtca aggctatcct gaattttgcc cctgtaaagc ttgatgtgcc tgaagatgta
    11701 aaggtccatt cggcggacct cacgttggag ctacagagcc ttgcgtttta tactcgctga
    11761 gcatgtgcga ggaatgtcgc aaggccagcg acgctataga actcgatcat gttgcccctc
    11821 gcgtgggcgc gggggacgaa ataagcagtt ggctgtcaaa ccatgtgaaa gccgggcggc
    11881 cgcccggctt tctgcatggt cggcgacagc tgcaaccgcg ggacgggaag cttgagtgcc
    11941 acgaataggt gttagactgc gctgagagcc catgtatgtg caataatcgg tgctcttgca
    12001 ttaaggttgt cactttacac catacacacg aaagggtagg caatgcgggg atagccttct
    12061 tcgtattcca tgccgatcat gcgatgagga taacgccatc atctcggcgg gatcagccaa
    12121 ggccttgcac ttgttgatcg aatatgttct ataggaaatg acgtcatggc aggatgcggc
    12181 tgagcgacgc ctatcgacgg agtagtcgct ctccaagggc gaagggcggt cgaaatgcgt
    12241 atttccccta agccaagggt ctttgtgcgc tcaatttcat cccagtctat ctatggtggt
    12301 ggttttaaca tggagttgaa ccaagagctg atgatcggat cagtctatgc cagagatgcc
    12361 atacactccc tatatggcgg tcaacgaggc ttgaggatca gcaccccaaa ggcagcaccg
    12421 gttgttcttt tgtttgcaag aaaaagttgc gactttcagg tgcggcacgg cgtcgactgc
    12481 ggctctcctg aaggagaaca cagctgcgcc ctgccagaag atgaattgcc gatcgcccat
    12541 actgcctcag ccgagctggc gccatacgtt tcagatccgg aggaaggatg gcgtgatgat
    12601 ggcttctacc attttcgagg agagaagcac gttcagttcc ttcgcggtaa tcgcgcagtg
    12661 cgtgaccatg ctcgtgacgg aaagctgctc tttctttttg aagagactac ccgcccggag
    12721 gggagacagg cgtggagatt catcggtccg atggaatgtg caggctatta cttcgcgcag
    12781 gcgtccgccg ggaggatggc gatcatgttc cggcttgctc ctgtatacgc gatcgatcgc
    12841 cttcggaaga attctcagga attggtgttg cattctgtgt tggcagagcg ctgcgcgttg
    12901 gatttcctca atagccgcca tggcgctatt gctgcgagcg ctgagagcac tcgtcccgga
    12961 catcacagca atgcctggaa atggtcgtca gactgcgccc tgattcagcg gtatgcgctc
    13021 gatcgcgctt tcggcttctg tgagagttgc ggtaagccgt cgccgttttc ggaccctgat
    13081 ggtgaaccat atctagagcc ccattacatg cttcgaccgt atgattccgg ggtggatcct
    13141 gtgcatggca tcgctgcatg ctgccccgaa tgtcaccgga tgatccatga tgcctggaat
    13201 gggcggagtc atgatgtggc cctggcccgc aagatagcac tgcttgaacg ctcagttgac
    13261 agaggcgact tcctggcggt tacagctgcg gtaattagga acgcagaagg gaaggtattg
    13321 cttactcagc gagggcatgg cgcacttgcc ggcaaatggg agtttcctgg cggaaagatc
    13381 gagccagggg agaccttgga ggcttgtttg gccagggaaa tcagggagga attgaacctg
    13441 gacattgcag atattcgacc ccttgtgtcc tcgttctatc agtatgatga ctctgccgtg
    13501 cgccttttcg ggctgtctgc caacgcagtt ggaaaccagc ttgagctgcg ggaacatatc
    13561 agcaaagcgt gggtatggcc ccatgaattg gaatcgtatg accttgcctc ggcagatttg
    13621 cccatagcgg ctgttgcggc caggttattg tgaccgtcaa gtccctgtgc ccattgcgcc
    13681 gtagagatgt gcaagcgtgc catgctcttg agcgggcgtt cgaatcgttg cgaacttcag
    13741 ttggttgacc ctgcatgcag ccaaggatat tattaatgct ggagcagagt aggaattgcc
    13801 gcgcttgcga tgtgggacta tgatggcagc acagtcaggg ttgttgttat gtgggggtga
    13861 acggatgttc aagattgttg aaaagtgcac tctcgcgccg ggcgtgcacc agtatgaagt
    13921 tgaggcgccg ctggttgcta aaggcgcgcg ccccggcaat ttcgtcgtgg tcagaacgag
    13981 cgaaacaggg gaaaggattc ccctgacaat tgcaggccac aatcgagaga caggcacgat
    14041 cacgctcgtc gtgcaggaag tgggaaagag caccatggaa atgggtatgc tcgaggtggg
    14101 cgactatttt aaggatgtag caggccctct tggccaggca acccgcatcg agaaggtggg
    14161 aactgtggtg gctgtggccg gagggatagg ggttgcaccg gtccttccac agatcagggc
    14221 gcataaggcc ataggcaatc atgtcatatc cattgtaggc gcgcgcactc gtgatctgct
    14281 cgttctcaga gatgaagtag aaaagttcag tgacgaagtg atttgggcca ctgatgatgg
    14341 gagccttgga taccatgggt ttgtcagtgg agcgctcgaa gagttgatcg gttcggggca
    14401 gaagatcgat gaagtaatcg caatcgggcc tgtcaggatg atgcaggctg tagcagatgt
    14461 aactaggaaa cacaacatac ccacaactgt cagcctcaat gcgatcatgg ttgatggaac
    14521 cgggatgtgt ggatgctgta gagtcacagt agatggtgaa gtcaagtttt cgtgtgttga
    14581 tggaccggag ttcgacggac acaaagtgga cttcgacgaa ctcgtatcga ggcagggttt
    14641 cttccgcgac aaggagcaaa ttgcaagaga gcgcttcgag gcagataggg gtgggtgcaa
    14701 gtgtcacgca aaatagtcga cagaactgag atgccgaagc agtcgccgga ggcaaggcgg
    14761 acgaatttca gcgaagtggc gctcggactc gacccagaat tggcaaggcg tgaagctgaa
    14821 cgatgcatcc agtgcaagac taggccatgc acgacagggt gccctgttga agttgacatt
    14881 ccagagttcg tcgcgcttat tgccgaaggt aagtatgacg aggctgctgc gaagataaag
    14941 gagaagaaca gcctgcccgc gatatgcggg agggtatgcc cccaggagag tcaatgcgag
    15001 agcctgtgca cgctggggcg caagtatgaa cctgtggcca tcggagcgct ggaacgtttt
    15061 gctgcagatt gggccagcaa gcgagcggtt gagaagcgcg aagcctgtgc aggcgatgtt
    15121 gactccgatt tgcagtgcgg gtgtgaacag aaagtatgcg agctggactc ccgcggatgc
    15181 gctcctacaa gcgattctgc ggcttcagcg gcgcgtgtgg cggtgatagg tacaggccct
    15241 gcagggctca cctgtgctgc agaccttgcc ctcaagggat acaaggtaga tatgtttgag
    15301 tctttgcacg acaccggagg tgtgttgcga tacggcattc cagagttcag gcttccaaag
    15361 tccatcgtcg acgaggaagt tgaatacgtg cgcagcctgg gcgtacgaat cttcaccaac
    15421 gtagttgtag ggctgacttt ggatttgcgc gaaatgctgg agtctggcca ataccaggct
    15481 gtgttcattg ctactggtgc aggcctcccg tatttcctcg gcatccccgg agagaacctg
    15541 aacggggttt attccgctaa cgagttcctt accagggtca acctgatgaa agcctacatg
    15601 tttccccaat acgatactcc tgtcaatatc ggatcgaagg ttgccgtggt gggcgcaggc
    15661 aatgttgcca tggattcggc ccgcacttct cttcgtcttg gcgccgagga agtcaagata
    15721 gtctatcgcc gctctcgggc tgagatgccg gcaaggcttg aggagatcga gaacgccgaa
    15781 gaggaaggcg tggagatgat gctccttgcc aatcctgtgc gcatcatatc agacggctgc
    15841 ggtaaagttt cagggatgga gtgtatccgt atggaactgg gtgagccgga cgcaagcggc
    15901 aggaggcgcc cggtgccggt ag
//
LOCUS       DAOSAI010000012        15518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_2603_length_15518_cov_4.133157, whole genome shotgun sequence.
ACCESSION   DAOSAI010000012 DAOSAI010000000
VERSION     DAOSAI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15518
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2603_length_15518_cov_4.133157"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..1747)
                     /locus_tag="PLM83_01095"
     CDS             complement(338..1747)
                     /locus_tag="PLM83_01095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOK70040.1"
                     /translation="MYDVIVVGGGSAGLWTAKTAAENGCKVLLIDKRAKDDIGRKISY
                     DSTPPYVFEQLGIPLPSGKELDDTGDVLTVISPNKKHRVEAAVPNVLFSRRLLGQRLA
                     GYAADAGVEFMYRTEVVGPVIEDGYVVGVRARHLDSCDEEEIRARIVVDASGFYAVIR
                     RKLPPEILVQDPQYCEDVVLGYREVRRIVGESNDCPPPEYRGYYDYLGYHGGYLWIVR
                     EENNWANIGIGVQDYPGNPGPVRPVQEFADDNPAIGPECMVRGSGDPMYIPLRSCQPQ
                     LSANGFMMVGDAACQVRPSTGYGWHTGLLAGDIAGKVAAEAVKKQDVSREALWDYDVG
                     WKRGLGAILASFDAIRIMCQSVPNDELDFLLAKGILGSEQIEALWNDKPFEVGTGKQV
                     SSFFAGIGHTGLLLKVKSVIDSADKLYKLFLEAPDSPEGFEEWNRERVEIFTNLRAKL
                     GVKVHEKDYFGEGMKHELK"
     gene            complement(1815..3047)
                     /locus_tag="PLM83_01100"
     CDS             complement(1815..3047)
                     /locus_tag="PLM83_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOK70041.1"
                     /translation="MGKKTCCTENDPFFIDYPGRIKSIQEAMANEGFDVYLGSRLRTI
                     SWTTDAFCPWRSYIVIPREGLPTLFTFVIDASRVADDTWLPEDNVRGYAPLGGQDQIT
                     QISDFIKDELKIRKGRLAYETGLSNYTPEGNLSQYEYEMFTAALPGLEFVNAHIIVDR
                     LSLIKDEGTINRFREASRYTDVGHEAVREALVNGGWKGKTETEIAGIAECAMRKAGSE
                     WAWSFTAGNEIASGYRTGYAGGACTPATRRELQPGDSLMVDLHAMYRLGLGDYAYNYL
                     IAPVTDRMRWHARNYIDVVQMCIDNYREGITPLGLASMVMEMAEERGFTDYVVPGFEH
                     GIGMMGDEWRIGLNTGPIKYWTDPNHVYQAGEMLICAVQYACPEEKIGFRYECPILIH
                     ENCCELMAEFPLDVEEID"
     gene            complement(3064..3465)
                     /locus_tag="PLM83_01105"
     CDS             complement(3064..3465)
                     /locus_tag="PLM83_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOK70042.1"
                     /translation="MEDKLKYLSPEWRDEAYRRLSAELDPEKMNYATTSLVSVYHNCP
                     DGKERYFFVSCADGAIGDVSVGEGEPPEAEFSISGDYDVFAKMSRGELGAQAALMSGK
                     LKLKGNMVKALKLSSLADRLNRVIATIPTQF"
     gene            complement(3514..4107)
                     /locus_tag="PLM83_01110"
     CDS             complement(3514..4107)
                     /locus_tag="PLM83_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK70043.1"
                     /translation="MKEQERNDEVMKAAFEVFAEYGYRKATLDDIAQRLGLTKSALYL
                     YSDGKRDLYEKCVGRALIEWQSYVADAVAKEENVCRKFVALCQSSFDWLLNNRDMQMI
                     LSRDPNVFPLFGEDDPYFEINQKSIKMLDDVLERGVAEGRFRPIDREVVTNLLFWIYK
                     MVIIGTCVTPDAVDVRRLINGGLDVILHGVFVPGECG"
     gene            4552..5379
                     /gene="nadE"
                     /locus_tag="PLM83_01115"
     CDS             4552..5379
                     /gene="nadE"
                     /locus_tag="PLM83_01115"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOK70044.1"
                     /translation="MDCKAVADVICDWLKENTLRAGCSGIVFGLSGGLDSAVMAGLGM
                     RAMPEGCLGVIMPCHSDPQDVEDADLVARYFNLPTITYDLGPIYDEHLANLQRLLDPY
                     VAAKDRRRLHAEDADSLPDGTGESASRLESSKVRMAEANLKPRLRMTALYYYANVLGY
                     LVAGTTNRSELTVGYFTKYGDGGSDLLPLGNLVKTDVRELAAYLGVPQSIIEKPPTAG
                     LWSGQTDEGEMGISYADLDRYILTGEASDEVRDRVEALNRSAQHKFVRPPIAPIPSR"
     gene            5577..6539
                     /locus_tag="PLM83_01120"
     CDS             5577..6539
                     /locus_tag="PLM83_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOK70045.1"
                     /translation="MQKALPATLAVMCIGALMMIGLSLSPPRSSAPIKPIANLAERIR
                     DAAVIAISTLAVLFIGTVLAFVIIERQQSGGKRIFRAPTHVPGWVRILSLALVMLAFV
                     AATYTPPTEHEKPTPGPEAKGGEKKAPAESVEEPESEYAPRPEPPLVVPAKVAPWPWW
                     KYGLIFAAIAFIAVIGVAVIGPQRTAVTPKPVASDALAIADMLSIEMVDFSDAAIEAI
                     EEEPDAQIAVLMCYLAFLSALEMGGAPAESHYTPEECALNAEKAFNVPRRSLSGLLAA
                     YQKAFFSTHDVTSQDKAAALASLRSLKSHIDSISVAQAASGQRG"
     gene            6546..7052
                     /locus_tag="PLM83_01125"
     CDS             6546..7052
                     /locus_tag="PLM83_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70046.1"
                     /translation="MSSRFDEDIRHKELVSFVEMILTLAAFALFSIALYLLMSPHKRP
                     LVPKISGVGCLYIACLRAWQGAKQGRENPMPWLVTQFNFWEWVDSWAPWRKRRASSPA
                     VCPQFDAFRRKVRLSQGRSRYMVRFLEEQEAPVDLIEHVKGGYHGNGERMTPDDISSV
                     LDVMEELL"
     gene            7064..8029
                     /locus_tag="PLM83_01130"
     CDS             7064..8029
                     /locus_tag="PLM83_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011250797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOK70047.1"
                     /translation="MIIQASAKCHEIISSVSAVIVGKDDELQFVLMSMIADGHLLLED
                     VPGVAKTLTAQCFAQALGLNFKRIQFVPDLLPGDITGSTIYDARREDFKFKPGPIFAN
                     LILADEVNRGTPKTQSALLEAMQERQVTVEGQSYVLDPPFLVIATQNPLEFEGTYPLP
                     EAQIDRFIARLRLGYPSPEAEREILSRRQDRRTDRAVARQAIEKSEFLQIQNAVEHVY
                     VSAEIQDYIVSITTETRNDRRVQLGASPRGSLALFKLARARALMYERDYVVPDDVKAV
                     AVPALAHRILLRPELWAERITGESIVEDALDRVPVPVADWARAVD"
     gene            8048..9364
                     /locus_tag="PLM83_01135"
     CDS             8048..9364
                     /locus_tag="PLM83_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOK70048.1"
                     /translation="MGSRLTEKGYVYMVSFGALVLASILLDRWELLLLASVFMFGLFA
                     VAYEQDEPEYSISVNVSHDRCFEGEQVLLEVSISSGSDIPLLEAHILLCDEENHVISS
                     RQLSLSMRAGEIRNFTFRVNMPARGMFYVGDTVSRSYTRSGLKSFGDRVWPGEACLVY
                     PRPEPIRVDWGLAARSRQHVGECPSRVPGEGLEISDIRAAMPGDPVKRVNWRATLRFG
                     KLYVNDSTRDRNIDVVIVVDGLADVGTSPSTYLDCASRAAASIAMGFLENRDRVGIVR
                     YAGAVDWAVPRPGRNQLYVILDRLARLYAVQSFVAPNMRLLPRSVLPPGSLVLVITPL
                     LDDRAIDMIVGLAARGCNPMVLYVSPIPFIEDQLKDSAEDQLAKRWWGLDHKAKLKKL
                     RALGLQVAEWDGNGSLDACLSHYFGGQGFRSAGRSPWQSASRGGWA"
     gene            9361..9990
                     /locus_tag="PLM83_01140"
     CDS             9361..9990
                     /locus_tag="PLM83_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70049.1"
                     /translation="MRKIDWLRWLPAFLCLLVVICVVGITPTVYVMIPAAAALALSLW
                     GTVAANFKPFGWSSACVLTACALSLMEKRPVAWFEPALMGLGVICFLGAAQINSVYQR
                     KDVSPDLMEGFEDKAACLEENDAQAEQPLIDSAPAVRSALAIGAQRAVAIVFAAMAFS
                     YMFFALAGVRAKQLEEGFFMMMLGTTVIGVSIYWLIRLAAPDAYKKPHR"
     gene            complement(9944..10963)
                     /locus_tag="PLM83_01145"
     CDS             complement(9944..10963)
                     /locus_tag="PLM83_01145"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOK70050.1"
                     /translation="MAKCVRGRGAQWVGLPGAWYHDNDIVGLGGPGLIISASRRTDIP
                     AFYGEWFMNRIRAGFCLVPNPYCPTKVSMVSLAKDDVDAIVFWSRDPRSMMPHLHELD
                     NMGYGYLFQYTINGYPRLLEPKMPDLGSAIATFVELASVIGPSRTFWRYDPIILSNIT
                     PPEYHIKMFSEIAAALEGSTMRVTVSLLDLYRKTKRRLEELEEISLWETEVPGAPGQL
                     KSDVLGLFAEIAAIARSHGMVITSCAEPYDLSACGIGHGACIDADTIRAALGVEVSRR
                     KDPGQRGECGCVVSRDIGVYDTCMRGCVYCYATDGTGTEKAHDPHAPALNGGVSCTRQ
                     ARLSG"
     gene            11130..13172
                     /locus_tag="PLM83_01150"
     CDS             11130..13172
                     /locus_tag="PLM83_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK70051.1"
                     /translation="MVSDGSSADIESKLSAEEIRYMLHGSDSELVLNSLPVGVALLRM
                     PGYILEMANSTFARMIGEVKSHSLAPESRFEEVLPKQWMNVIIPLVDRAIETKNRVTV
                     CKARSGAEEGGSDAYWLIACQPVPNGASNTHHAIILSQDVSEQSVLLKRLKALSALGS
                     SLSRGYSLDEFLADGVGKCSAAISADYCAAFLLDQDTPRLLRVAESKPLCWLPSEITF
                     DSPEEWPCGIETSASAYVSLPQANAREARWLGSIGAYGYLRIPLMTVGRPIGGMAAVF
                     KKIGYVPPQEDIEFAELVAERCAMAIAANAAIAEYGRLLAAEQEAHRAATAKAAQMEA
                     LLESLECGVVAYDRNGYVILTNRACEEMLDVGKPQSRYGDDIAFGITLEKLDGEAVSL
                     KEMPYCKLLREAALADQHYMMHYTDGSYRVISFNGGIVRDAKGQMSSAIIIMRDITRM
                     YEMEKTREEVIRLISHDLRSPLTLILARAQMMERVADKPDAVRKNAMGIVLSARYMNI
                     MISDLSDSFRIQSGKITLKRQVFDLAKFISDCVERWLDAPETTRLKLDIEPNLPPVYA
                     DPVRLERILTNLTTNAFKYSTGDSEVLVEAARYQDSDDQALISITDHGQGIAEDELPH
                     IFERYWRASEARERSEGMGLGLYICKELVEAHGGRIWVHSVQGQGSTFFFTVPFAG"
     gene            complement(13431..14660)
                     /gene="grdD"
                     /locus_tag="PLM83_01155"
     CDS             complement(13431..14660)
                     /gene="grdD"
                     /locus_tag="PLM83_01155"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HOK70052.1"
                     /translation="MTDDAKRRIAELFEKLADALESGADLAGLAEGAGGRRPRIGLTV
                     LGSEHGPEEIIDGALRALRQYSDLEVALIGPSELAESVRGVAGLRLYPAASEEEQHRE
                     MERLLDAGEIDACVTMHYNFPIGVSTVGRVVTPSRGREMFIATTTGTSGTERVENMVK
                     NAVYGIVAAKASGVVEPTVGVLNIDGARQVERALRKLSDAGYPIQFAESARADGGAVM
                     RGNDLLMATPDVMVCDSLTGNLLMKIFSAASTGGDYEATGYGYGPGIGEGYGRIIMIL
                     SRASGAPVVAGAIRYAADLVKGGVHEVARVEFAAARKAGWPEIGLPTGAGASASAPAQ
                     GDAAAAAESVEPPPKKVVTSDIAGVDILQLEEAVSVLWGAGIYAETGMGCTGPVIMVA
                     DEDKDAARKALESSGYL"
     gene            complement(14671..>15518)
                     /locus_tag="PLM83_01160"
     CDS             complement(14671..>15518)
                     /locus_tag="PLM83_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5940 domain-containing protein"
                     /protein_id="HOK70053.1"
                     /translation="KAIGEMAGCVNASGCDVRGFCAAPAHAMVNAAGLVQSQVFRNVA
                     VVGGGATAKLGMNGRDHVKKGLPLLEDVLGGFAVLVSANDGTSPIIRTDAVGRHKIGS
                     GSSPQAVMTAIVVDPLERVGLSLKEVDKYSAEMQNPEITEPAGAGDVPTSNLKMIAAL
                     AVKRGEIARGDMGTFVKKHGMPGFAPTQGHVPSGVPSLGYLCDEISAGRIERAQIIGK
                     GSLFLGRMTNLFDGISFLIQKNPGKCGSRDRAAAAGMAGVSEHELRKIMADALRGAAQ
                     TLVES"
BASE COUNT         3441 a         4726 c         3952 g         3399 t
ORIGIN      
        1 cctgcgcgcc atcgacaacc aagtccatct gcacagacca ttttccattc acctcgccca
       61 ccaggcggta gtgaagagcc tcaatggcaa cggttgtagg tgccaacgcc accaatatta
      121 acccgacaac taacatcccg acaatcctgc gccgagcctt catctgtctc tcctccaatc
      181 gtagtatggg gccattatcg cacgccgtcc tcaaggctcc gcctcgtcag ataatgggca
      241 acgcggcaga gagcatgtcc cctgccgcgt tgcccagcga aggtaggttc gcatgacgcc
      301 gttgacaggc attgccgggc gtctttccac ctgctgttca tttcaactcg tgtttcatgc
      361 cctcgccgaa gtaatctttt tcatgcacct taacgcctag tttggccctg agattagtaa
      421 atatctctac acgctctcgg ttccattctt caaagccctc aggcgagtca ggcgcctcca
      481 aaaagagctt gtaaagcttg tccgcagaat cgataacgga tttgactttc aaaagaagcc
      541 ctgtatgacc tattccagcg aagaaggatg aaacctgctt tcctgttccc acctcaaacg
      601 gcttgtcatt ccacaaagcc tctatctgtt ctgatccgag gatccccttg gcaaggagaa
      661 aatccaattc gtcattggga acgctctggc acatgatgcg gatcgcatca aacgaagcga
      721 gaatcgcccc gagcccgcgt ttccacccaa catcataatc ccacaaggcc tcccggctca
      781 cgtcctgctt cttcactgcc tcggccgcca ccttgccggc aatatccccg gcaagaagac
      841 ccgtatgcca accatatccc gtagacggcc tgacctggca cgccgcgtcg ccaaccatca
      901 tgaacccgtt ggccgaaagc tgcggttggc aagagcgaag cggtatgtac atgggatcgc
      961 cactccccct gaccatgcat tcaggcccaa tggcgggatt gtcgtcggcg aattcctgca
     1021 ccggccttac cggacccggg ttccccggat aatcctgaac gcctatgcca atgttggccc
     1081 agttgttctc ttcgcgaacg atccacaagt aacctccatg gtaccccaga tagtcgtaat
     1141 atccgcgata ttccggcggc ggacagtcat tggattcccc tactatcctc cgcacctccc
     1201 tgtatcccaa gaccacatcc tcgcagtact gcggatcctg aacgaggatt tcgggaggaa
     1261 gcttcctccg tatgactgcg tagaaccccg atgcgtccac tacgatcctc gcgcggatct
     1321 cctcttcgtc gcaggaatcg agatgcctag ctctcacacc gaccacatag ccatcctcaa
     1381 tcacagggcc cacaacctca gtcctgtaca tgaactccac accggcgtcg gcagcatagc
     1441 cggcaagcct ctgtcctagg aggcgacggc tgaaaaggac gtttggaaca gccgcctcga
     1501 ccctgtgttt cttgttaggt gagatcacag tgagcacatc gcctgtatca tccagttcct
     1561 tacctgacgg aagcgggatc cccaattgtt cgaaaacata gggcggggta gagtcgtagc
     1621 tgatcttcct tcctatgtca tcctttgccc tcttgtcgat gagcaagacc ttgcatccgt
     1681 tctccgcggc cgtctttgca gtccacaagc ctgcgcttcc cccgccgaca acgattacat
     1741 cgtacatcaa gcgaccctcc tcgcaaacgg atattcttcg gccctatagt ccctctacga
     1801 gcgtccgttc aggcctaatc tatctcctca acatcaagcg ggaactcggc catcagctcg
     1861 cagcagtttt cgtgtatgag aatcgggcat tcatacctga acccaatttt ctcctccggg
     1921 cacgcatatt gcaccgcgca tatgagcatt tccccggctt ggtacacatg gttgggatcc
     1981 gtccagtact tgataggccc ggtattcaag cctatcctcc actcgtctcc catcatcccg
     2041 attccatgtt caaaccctgg aaccacgtaa tcggtgaacc cgcgttcctc cgccatctcc
     2101 atcaccatac tggccagtcc cagaggagta atcccctccc ggtagttgtc gatgcacatc
     2161 tgcaccacgt ctatgtaatt acgcgcgtgc cacctcatcc tgtcggtaac aggagctata
     2221 aggtagttat aagcataatc cccaagaccc agcctgtaca tggcgtggag gtctaccatg
     2281 agggaatcgc cgggctgcag ttccctacgg gtcgcaggcg tgcatgcccc tcccgcatat
     2341 cccgtgcgat accctgaagc tatctcattg ccggcagtga aagaccaagc ccactcgctc
     2401 ccagccttcc tcattgcaca ctccgcgatc cctgcgatct cagtctcggt cttgcccttc
     2461 cagcccccgt tcacaagggc ctccctgacc gcctcatggc cgacgtcggt gtaccgcgag
     2521 gcctcccgaa aacggttgat ggttccctca tctttgatca gcgacagcct gtctacaatg
     2581 atatgggcat tgacgaactc gaggcccggc aacgcagcag taaacatctc atactcatac
     2641 tggctgaggt tgccctcagg cgtgtaattg gaaaggcctg tctcgtaagc gagccgccct
     2701 ttccttatct tcagttcatc ctttataaag tcggaaatct gcgtgatctg atcctgcccg
     2761 cctaagggag cataacccct aacattgtcc tccggaagcc atgtgtcatc agccacccgt
     2821 gaggcgtcga tgacgaaggt gaaaagcgtt ggcagccctt ccctaggaat cacgatatag
     2881 cttcgccacg ggcaaaaggc atcagtagtc cacgaaatgg tgcgcaggcg agaaccgagg
     2941 taaacatcaa agccctcatt ggccatggct tcctgtatgc tcttgatcct cccaggataa
     3001 tcgatgaaga acggatcgtt ctctgtacaa catgtcttct ttcccatgat ctcgcctcct
     3061 cgattagaat tgagtgggaa tcgtggctat tacgcgattg agcctgtcag ccagcgacga
     3121 caatttgagg gctttaacca tgttcccctt cagtttcagc ttgccgctca ttagggcagc
     3181 ctgagctccc agctcgcccc tggacatttt ggcaaacacg tcatagtcgc cggaaattga
     3241 aaactccgct tccggcggct cgccctcgcc cacagacaca tctccgatag ccccatccgc
     3301 gcagctcaca aagaaatagc gttcctttcc gtcagggcag ttgtgataga cggataccaa
     3361 agacgtagtt gcatagttca tcttctccgg gtcgagctcg gctgaaagcc tcctgtacgc
     3421 ctcgtcacgc cactcagggc tgagatattt gagcttgtct tccatagtga ccctcctccc
     3481 ttgcaactcg cgtattccct cgtctccccg ttcttacccg cattcaccgg gcacaaacac
     3541 tccgtgcaat atcacatcga gcccgccgtt gatgaggcgc ctaacatcta cggcgtccgg
     3601 ggtgacgcag gttccgatta tgaccatctt gtatatccaa aaaagcaggt tcgtcacaac
     3661 ctcccggtct attggcctga aacgtccttc agccactccg cgctctaaca cgtcatccag
     3721 cattttgatg gacttttggt tgatctcgaa gtacggatca tcctctccga atagcggaaa
     3781 gacattgggg tcgcgactta ggatcatttg catgtcgcga ttgttcagaa gccaatcgaa
     3841 ggacgactgg cataatgcaa caaactttct gcagacattc tcttccttcg ctaccgcatc
     3901 ggccacgtat gattgccact ctatcagcgc acggcccaca catttctcat ataggtcccg
     3961 cttgccgtcg gaatacaggt aaagagcgct ctttgtcaat cccagtcgtt gagcgatatc
     4021 atcgagcgtc gctttcctgt aaccgtattc tgcgaacacc tcaaacgcgg ccttcataac
     4081 ttcatcatta cgttcttgtt ctttcacgta ttcagcctcc atgccgccat ccgtccactt
     4141 aattatttaa cgaatcgttc atttattaat attctacaca caatttttga gcgcgtcaat
     4201 cccagcaatc tactatgagt ccaatctcaa atgccatatt gcccataaag ttctcgactc
     4261 agcgccttcc tctgtcccgt ggatcaaagg aacgggaaca tgcggttcaa gatgcggagc
     4321 cagcctcgcc ctagcccata ggactaatgc caagaaagcc agagccacat ttgccacttc
     4381 ttccccagcc cacgcgtgga tgcagcggga ttgatgacat cctttcagca tgacggatat
     4441 cgcgtcatgg gcccggggat gtgttatcat gtctacgtgg gctgttgtgg atcagcccac
     4501 agcagactat tatgatgggc gtgcgtaggc gccgggcaag gtgatggaag tatggattgc
     4561 aaggcagtag cagatgtcat ctgcgattgg ctaaaggaga acacgctgcg cgcaggatgc
     4621 agcggaatag tttttggact ctccggaggg ctcgactcag cagtaatggc cggattgggc
     4681 atgagggcaa tgccagaggg atgccttggc gtaatcatgc catgtcactc agatccacag
     4741 gatgtggagg acgcagactt ggtggcccgc tatttcaacc tgcctacgat cacgtacgat
     4801 ctgggcccga tctatgacga acacctcgca aatcttcaac ggcttctcga tccttatgtt
     4861 gctgccaagg atagaaggcg gctccatgca gaagatgcag attctctccc cgatggaacc
     4921 ggcgagtcag cttcacgcct ggaaagctcg aaagtccgaa tggctgaggc aaacctcaag
     4981 ccgcgtctga ggatgaccgc gctctactac tacgccaacg tcctcggcta tctggtggca
     5041 gggacgacca acagaagcga gttaacagta ggttatttca caaagtatgg agacggcggg
     5101 tccgacctat tgcccttggg caacctggtc aagaccgatg tgcgcgaact cgcagcatat
     5161 ctgggcgtac cccagagcat tattgaaaag ccccctactg ccggcctgtg gagcggccag
     5221 acagacgaag gcgaaatggg catctcctat gccgacttgg atcgctacat actcacagga
     5281 gaagcctcgg acgaagtgcg ggaccgagtg gaggcgctaa atcgcagcgc tcaacataag
     5341 ttcgtgaggc cacctattgc ccccatcccc agccgctaaa tcatgaggtt ggccccacta
     5401 tccatctgcg catttgccga gcatcaactt gcatggcgcc cagggaatcc catagggctt
     5461 cttatgtgtt ataattctta aaagctcgcc ccccgcccgt tccacaagac ccaaatctcc
     5521 tgcgatttca ggtagccctg ggccgggcct gggaaagaac ggagttgctt caatcgatgc
     5581 aaaaggctct tcccgcaaca ctcgcagtaa tgtgtatcgg ggcactgatg atgataggcc
     5641 tctctctaag cccgcctcgg tcttctgcgc ccatcaagcc cattgccaac ctagcggagc
     5701 gcattaggga tgccgcggta atcgccatat ccacgctcgc agtccttttc atcggaaccg
     5761 tgcttgcctt cgtcatcatc gaaaggcagc agagcggcgg caagagaatc ttccgcgcgc
     5821 caacccatgt ccccggttgg gtccgcatcc tatcactggc cttggtcatg ctcgcgttcg
     5881 tggccgctac atacaccccg cccacagagc atgagaagcc cacgccggga ccagaggcaa
     5941 aaggaggcga gaagaaggcg ccggcagagt ccgtcgagga acctgagtcg gagtatgccc
     6001 cgaggcctga acctccttta gtcgttcctg ccaaggtggc cccgtggccc tggtggaaat
     6061 atgggctgat ctttgcagcc atagccttca tcgcagtcat aggcgtcgcc gtaatcggcc
     6121 cacagcgcac cgccgtcacc ccgaaacctg ttgcgtcaga cgctttggca atagcggaca
     6181 tgctgtccat agagatggtt gacttttcag acgccgccat agaggcgatt gaggaagaac
     6241 cagatgccca gatagcggtc ctcatgtgct atctcgcttt tctgtctgca ttggagatgg
     6301 gcggagctcc cgccgaatcc cactacactc ctgaagaatg cgctctcaac gccgagaagg
     6361 ccttcaatgt gccgcgaaga tccctttcgg gtctgcttgc agcatatcag aaagcttttt
     6421 tcagcaccca tgatgtgacg agccaggaca aggcagctgc cctggcttcg ctgcgaagcc
     6481 tcaagtcgca tatagattcc atttctgtcg cacaggcagc cagtggccag agggggtaat
     6541 catccatgtc ttccaggttc gatgaggata tcagacacaa agaactcgtt tcttttgtgg
     6601 agatgattct gacgcttgcg gcctttgccc tcttttcaat agcgctctac ctgctgatgt
     6661 ctccgcacaa acgccccctg gtccccaaga tttccggggt aggctgcctc tacatcgcct
     6721 gcctccgcgc atggcaagga gcaaaacaag ggcgggagaa tcccatgcca tggctggtaa
     6781 cgcagttcaa cttctgggaa tgggttgact cgtgggcgcc atggcgcaag cggcgcgcat
     6841 cctcccctgc agtctgcccc caattcgacg ccttccgccg gaaagtcagg ctttctcagg
     6901 gccgaagccg ctacatggtc cgcttcttag aagaacaaga ggccccggtc gatctaatcg
     6961 aacatgtcaa aggcggctac catggcaacg gagaacgcat gacgcctgat gacatatctt
     7021 cagtccttga tgtgatggag gagttgctat gagcatttca gacatgataa tccaggcgtc
     7081 cgcaaaatgt cacgagatca tctcgtcagt atccgcagtt atcgttggca aagatgacga
     7141 acttcagttt gtcctgatga gcatgattgc cgacgggcac cttctcctgg aagacgtgcc
     7201 gggagtcgcc aaaactctaa cggcccaatg cttcgcccaa gctcttggcc tgaacttcaa
     7261 gagaattcag ttcgtgcccg atcttctccc gggagatatc accggaagca cgatatacga
     7321 tgccaggcgt gaggatttca agttcaaacc agggccgata ttcgccaatc tcatcctcgc
     7381 agacgaggtg aaccggggca ctccaaaaac ccagtcggcc cttctcgaag cgatgcaaga
     7441 gaggcaggtt actgtggaag gacaaagcta cgtgcttgac ccgccttttc tcgtaatagc
     7501 cacccaaaac cccctcgaat tcgaagggac ctatcccctg ccggaagccc agatagacag
     7561 atttatcgcc aggcttcggc tgggataccc gtcccccgaa gcagagcgcg agatcctctc
     7621 gcgcagacag gaccggcgca ccgacagagc agtcgcgcgc caggccatcg agaaaagcga
     7681 gtttctgcag atccaaaacg cagtggaaca tgtatatgtc agtgccgaga tccaagatta
     7741 catagtctcc atcacaacag aaacccgcaa cgaccgcaga gtccagctgg gtgcatcgcc
     7801 cagaggcagc ctggctctgt tcaagctggc gagagcccgg gcactcatgt acgaaagaga
     7861 ctacgtggtg cccgacgacg tcaaagcagt ggcagtcccc gccctcgccc accgcattct
     7921 tctccggccc gagctttggg ctgagcgcat tacgggcgaa tccatagtgg aggacgcact
     7981 cgatcgggtg ccagtccctg tcgctgattg ggcaagagct gttgactaga ggcggaggtt
     8041 gatcggcatg ggctcgcggc ttactgaaaa aggctacgtc tatatggttt ccttcggagc
     8101 tctcgtcctg gcaagcatac tcctggaccg ttgggagttg ttgctgctcg cttccgtgtt
     8161 catgttcggc ctcttcgccg ttgcatacga gcaggatgaa cctgaatact ccatctcagt
     8221 caatgtctcg catgacagat gctttgaagg cgaacaggtg ttgctggagg tctcgatctc
     8281 ttctggatcc gacatccctc ttcttgaggc ccacatcctc ctgtgcgatg aagaaaacca
     8341 cgtgatctct tcgagacaac tctccctctc tatgagggcg ggagagatcc gcaattttac
     8401 attccgcgtt aacatgccgg cgcggggcat gttctacgtg ggggacacag tctcacgctc
     8461 gtacaccagg tcagggctga agtccttcgg cgaccgcgtg tggccgggcg aagcttgcct
     8521 ggtctatccg cgaccggagc ccattcgcgt cgactggggc ctggcagcca gatcccgcca
     8581 gcacgttggg gaatgtcctt cgcgagtgcc cggcgaaggg ttggagatat cggacattcg
     8641 cgcagccatg ccaggtgacc ccgtcaaacg agtcaactgg cgggcaacgc tcaggttcgg
     8701 gaagctctac gtcaatgaca gcacgaggga tcgcaacatc gatgtggtca tagtagtaga
     8761 cggtctcgct gacgtgggaa catccccctc aacttacctc gactgcgcct cgcgcgcagc
     8821 cgctagcata gccatggggt ttttggaaaa ccgggacagg gtcggaattg tgagatatgc
     8881 gggcgcagtg gactgggctg tgccgcgtcc cggccgcaac cagctatacg tgatcctcga
     8941 caggctggcg cgcctttacg ccgtccagag ttttgtggcg cccaacatga ggcttcttcc
     9001 cagatcagtc ctgccgccgg gatcccttgt tctcgtcatc accccgctcc tcgacgacag
     9061 agccatcgac atgatagtgg ggctggcggc ccggggttgc aatccaatgg tcctgtatgt
     9121 ctcgcctata ccattcattg aagatcagct caaagactct gccgaagatc agcttgcaaa
     9181 aagatggtgg ggcctcgacc acaaggcaaa attgaagaag ctcagggcgc tcggtcttca
     9241 agttgcagaa tgggatggca acggctcgct cgatgcctgc ctcagccatt acttcggcgg
     9301 acaaggcttc agatctgcag ggcggagccc ttggcaaagt gcttcgaggg gaggatgggc
     9361 atgagaaaga ttgactggct tcgctggttg cctgcgtttc tctgtctcct ggtagtcatc
     9421 tgcgtggttg gcatcacgcc tacagtctat gtaatgatcc cggcagccgc cgcgcttgcg
     9481 ctctctttgt gggggacggt cgcggcaaat ttcaagccgt tcggctggag ctctgcttgc
     9541 gtgctaaccg catgcgcctt gagccttatg gaaaaaaggc ctgtagcctg gtttgagccc
     9601 gccctcatgg ggttgggagt catctgcttc cttggcgcag cccagatcaa ttctgtatat
     9661 cagagaaagg atgtctcgcc cgatctcatg gagggtttcg aggataaagc tgcgtgcttg
     9721 gaagagaatg atgcgcaagc agaacaacca ctgatcgact cagctccggc cgtccgctcg
     9781 gcgctcgcca tcggcgcaca gagagcggtc gcaatcgttt ttgcggccat ggcgttttca
     9841 tacatgttct ttgccctcgc tggcgtcaga gcaaagcaac ttgaagaagg attcttcatg
     9901 atgatgctgg gcacaactgt aatcggggta tcgatatact ggctcatccg cttagccgcg
     9961 cctgacgcgt acaagaaacc ccaccgttga gcgcaggcgc atgcgggtca tgcgcctttt
    10021 ccgtccccgt cccatccgta gcgtagcagt aaacgcatcc gcgcatgcac gtatcataga
    10081 cgcctatgtc gcggctcacc acacatccgc attcgccacg ctggccggga tctttccgcc
    10141 tgcttacttc tacacccaaa gccgctcgga tcgtgtcagc gtcaatacac gctccatggc
    10201 ctatcccaca tgcagacaag tcatacggct cagcgcaact tgtgatgacc atcccatgac
    10261 ttctggcaat cgcagcaatc tcggcaaaaa gacccaacac atcgcttttg agctgcccag
    10321 gagcaccggg aacctccgtc tcccacagcg agatctcttc caattcctca agacgccgct
    10381 tcgtcttgcg ataaaggtcg agcagactca ccgtaaccct catggtgctt ccttcaagag
    10441 ccgcagcgat ttccgaaaac atcttgatgt ggtattcagg aggtgtgatg ttgctcagga
    10501 ttatcggatc atatctccaa aaagttctgg aggggccgat cacggaagca agctcgacaa
    10561 atgtggcaat cgcagaacct aggtccggca tcttaggctc cagcagtcta ggatagccat
    10621 tgatcgtata ctgaaacaga taaccatatc ccatgttgtc aagctcgtga aggtgcggca
    10681 tcatagatct agggtcgcgc gaccagaaga cgatcgcgtc gacatcatct ttggcaaggg
    10741 acaccatgga cacttttgtg ggacagtaag ggttggggac gagacagaac ccggcccgaa
    10801 tcctgttcat gaaccactcg ccatagaatg cagggatatc cgtccgtcta cttgcactga
    10861 ttatcaatcc cggtcccccc aatcccacaa tgtcattgtc atgataccat gcgccgggca
    10921 accccaccca ctgcgcaccc cggcccctca cacatttagc catgtcagct tcttcaaccg
    10981 tacattcgga cagcgcatat gggtataaaa cacaaaccat tgatactctt ggaaggaaaa
    11041 ctattatttc cgttgaacaa tatataacct gccattgttg ctcacattgc ctccatatgt
    11101 tatctgcgtt ttgggaagga gttgggcata tggtgtcaga cggttcctca gctgatatag
    11161 aaagcaagct ttccgcagaa gagattcgct atatgctgca tggatcagat tcggaactcg
    11221 tcctcaattc actgcctgtg ggcgttgctc tcttgcgcat gcccggctac attcttgaga
    11281 tggctaactc gacttttgca aggatgatcg gagaagtcaa gtcgcattct ctggctccag
    11341 aaagccgatt cgaagaagtc ctgccaaagc agtggatgaa tgtgatcata cccctggtcg
    11401 accgagctat agagaccaag aatcgtgtca cggtgtgcaa ggcccgatcg ggcgccgaag
    11461 aaggaggctc cgacgcttac tggctcatcg cctgccaacc tgtaccgaac ggggcctcca
    11521 acacgcacca cgcaatcatc ttgtcccaag atgtttccga gcaatctgtc ctcctcaaga
    11581 gactaaaggc cctcagcgcc ctcggatcga gcctcagccg cggctatagt ctggacgaat
    11641 tcctcgccga tggagtcggc aaatgctctg cggccatctc cgcagactac tgtgcagctt
    11701 ttctgctaga tcaagacacc ccccgcctcc tgcgcgtcgc tgaatccaaa cccttgtgtt
    11761 ggctcccttc agagatcaca tttgattcgc cagaggaatg gccatgtggg atcgagacga
    11821 gtgcctcggc ctatgtatct ttaccccagg cgaacgcacg cgaagcaaga tggctaggct
    11881 ccatcggcgc ctatgggtac ttgcgcattc cgctcatgac agtaggaagg ccaatagggg
    11941 gcatggcggc ggtcttcaag aagatcgggt acgttccgcc ccaggaagac atcgagtttg
    12001 cggagttggt agctgaacgg tgcgccatgg cgatcgccgc caatgcggca atagcagaat
    12061 acggtcgact cctcgccgct gagcaggaag cgcatcgcgc tgcaacagca aaggcggcgc
    12121 agatggaggc gctcctcgaa agcctcgaat gcggagtggt tgcgtacgac cgcaacggct
    12181 acgtgatact gacgaaccgt gcgtgcgaag aaatgctcga cgttggaaag ccccagtcca
    12241 ggtatgggga tgatatcgcg ttcggaatca ctctcgaaaa gcttgacgga gaagcggttt
    12301 cattgaagga aatgccttac tgcaagcttt tgagagaagc ggcccttgcc gaccagcact
    12361 acatgatgca ctacacagac ggctcctacc gggtcatcag cttcaacgga ggcatcgtgc
    12421 gggacgcaaa gggccaaatg tcgtctgcca taataatcat gcgggacata actcggatgt
    12481 atgaaatgga gaaaacccga gaagaagtga tcaggctcat ctctcacgac ctccgctcgc
    12541 cactgaccct gatacttgcg cgagcgcaga tgatggaacg cgtagccgac aaacccgacg
    12601 cagtccgtaa gaacgccatg ggaatcgtct tgtccgccag gtacatgaat atcatgatat
    12661 ccgacctttc ggattctttc cgaattcagt cgggcaagat cacgctgaaa cggcaagttt
    12721 tcgatcttgc gaagttcatc tctgattgtg tagagcgctg gcttgatgcg ccagaaacaa
    12781 cgaggctgaa gcttgatatt gaaccgaatc tcccgcctgt atacgccgat cccgttcgcc
    12841 tggaacgaat cctgacgaac ctgaccacca atgccttcaa gtattctacc ggcgattcag
    12901 aagtgcttgt ggaagccgct cgctaccagg actccgatga tcaagcactc atatccataa
    12961 ccgaccacgg acagggcatt gccgaagacg agctgcccca catattcgaa cgctactggc
    13021 gcgcctccga agctcgagag agatcagaag ggatgggtct cgggctgtac atatgtaaag
    13081 agctcgttga ggcacacgga ggaagaatct gggtgcacag cgtgcagggc cagggaagta
    13141 ctttcttttt cactgtgcct tttgccggat aacctgcgcc gcccgtgcgg ttcccacccg
    13201 ccgagctcac caaacccctc gccgcaatag aggatattgc gaattatgcc gcaattgcac
    13261 gccaaagaat actcttcaaa acacagcaag aactgataca ttcgagaatc gccgaccacc
    13321 acaagcaaat agctctgcct tgctcgacct tggagtctgt gctacccgct tgcaatcgat
    13381 tggaagaggg gcggatcttg tgcccgcccc tcttcgcctg caaaggcctt ctataggtat
    13441 ccgctagact ccaaagcctt cctcgccgcg tccttatctt cgtctgctac catgattaca
    13501 gggccggtgc atcccatacc ggtctcggca tatatccccg cgccccacag aacgctcaca
    13561 gcttcttcca gctgaagaat gtccactccc gcaatgtcgg aagtgacgac tttcttaggc
    13621 ggaggctcta cactctcagc ggcagcagcc gcgtcgccct gagccggcgc agacgccgaa
    13681 gctcccgctc cggtggggag ccctatctcg ggccagcccg ccttccgggc ggcggcaaat
    13741 tcaaccctgg ccacctcgtg aactccaccc ttcaccaggt cagccgcgta tctgatcgct
    13801 ccagccacca ctggcgcgcc cgatgcgcgc gagaggatca tgatgatccg cccgtatccc
    13861 tcgccgatcc cgggaccata accgtacccg gtcgcttcgt agtccccccc cgtgcttgcg
    13921 gccgagaaga tcttcatcaa gagattgcct gtgaggctgt cgcacaccat cacgtcagga
    13981 gtagccatca gaagatcgtt gccacgcatg accgcgcctc cgtcagcccg cgccgattcc
    14041 gcaaactgga tggggtatcc ggcatcagat agctttctca aagctctctc aacctgcctt
    14101 gccccatcta tgttgagcac ccctactgta ggctcaacca ccccgctggc tttcgccgct
    14161 acgattccgt acacagcgtt cttcaccatg ttttcgacgc gctctgtccc tgatgtgcca
    14221 gtggttgtgg ctatgaacat ctcccgtccc ctacttgggg tgacaacccg ccccactgtg
    14281 gagacgccta ttgggaagtt gtagtgcatg gtgacacatg catcgatctc gccggcatcg
    14341 agcaatcgct ccatctcgcg atgctgctct tcctcgctgg ccgccgggta cagcctgagc
    14401 ccggccacgc cccgcaccga ttccgccaac tctgaaggcc ctatcaaagc cacttccagg
    14461 tcggaatatt gacgcagcgc acgtagggcc ccgtcgataa tctcttcggg cccgtgttcg
    14521 ctgccgagta cagtcaggcc gatcctcggc ctgcgtccgc ctgcgccctc cgccaaaccg
    14581 gcaaggtccg ctccgctctc gagagcatcg gcaagtttct caaagagttc agctattctt
    14641 cttttagcat catcagtcat ggcagccacc tcacgactcc actagcgtct gcgcagcccc
    14701 gcgcaacgca tctgccatga tcttgcggag ctcgtgttcg gatactccgg ccatccccgc
    14761 tgcagcagcc cgatcccggg aaccgcattt gcccgggttc ttctggataa ggaatgagat
    14821 cccgtcaaag agattggtca ttctgcccag gaagagagag ccctttccga ttatctgggc
    14881 gcgttcgatt cggccggcac tgatctcgtc acacagatat cccagggacg gaactccaga
    14941 aggcacatgc ccctgcgtag gagcgaaccc gggcatacca tgctttttca cgaatgttcc
    15001 catatcaccc ctggcgatct cgcccctctt taccgcaaga gctgcgatca tcttcagatt
    15061 ggatgtgggc acgtcccctg cacctgcagg ctcagttatc tccggattct gcatctcggc
    15121 agaatatttg tccacttcct tcaagctgag accgacccgt tccagggggt ccacaactat
    15181 ggcggtcatc accgcctgcg gagaagagcc ggaaccgatc ttgtggcgtc ccaccgcgtc
    15241 agtgcggatt atggggcttg tcccgtcatt ggcagaaacg agaacagcaa atcccccgag
    15301 aacgtcctct agcagaggaa ggcccttctt gacgtggtcc ctcccgttca tgcccagctt
    15361 cgccgtggcc ccgcctccga cgacagccac gttcctgaat acttgcgact gcacgagtcc
    15421 cgcagcgttc accatggcat gggccggcgc cgcacagaat ccgcgaacat cgcatccgga
    15481 agcgttcacg cagcccgcca tctcgccaat ggccttgg
//
LOCUS       DAOSAI010000013        15113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_2690_length_15113_cov_3.438637, whole genome shotgun sequence.
ACCESSION   DAOSAI010000013 DAOSAI010000000
VERSION     DAOSAI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15113
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_15113_cov_3.438637"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PLM83_01165"
     CDS             <1..633
                     /locus_tag="PLM83_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70054.1"
                     /translation="KLFDDVAAMDERVAELLTRVGLYPEYAQRYPHEFSGGQRQRIAV
                     ARSIAVNPRLLICDEPTSALDVSVQSQIINLLKEIQRETGMSLIFISHNLDVVHHMSD
                     RIMVMYLGNVVESAPASELFDEPAHPYTSALMSAIPSWNPKDRKLAKVKLEGEPPSPI
                     NPPSGCPFHPRCPHAMELCHEIKPEPQEVNDGHLCACHLHARADAEVAAR"
     gene            702..1610
                     /gene="murQ"
                     /locus_tag="PLM83_01170"
     CDS             702..1610
                     /gene="murQ"
                     /locus_tag="PLM83_01170"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOK70055.1"
                     /translation="MDYADLATEETNPRSRGLDRMSTVQILEVMNEEDRRVPEVVARA
                     IPEIAKAVDMIVARMAAGGRLLYVGAGTSGRLGILDAVECVPTFGVDPNQVSAVIAGG
                     PGAVFHSVEAAEDDESSGVRDVAESITELDVVVGISASGVTPYVQGALKAAKEVGAGT
                     VAIVCNHADKLDLDVDVVISLPVGAEVLTGSTRLKAGTAQKLVLNMISTATMIKLGKV
                     YDNLMVDMQATNRKLKRRAVRIVSIATGLDEEVSEDLLDKAGGNIKTAIVMALASVNR
                     REAERALEKACGHTRNAVQAAIERTR"
     gene            1617..2660
                     /locus_tag="PLM83_01175"
     CDS             1617..2660
                     /locus_tag="PLM83_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOK70056.1"
                     /translation="MPAERFPPLLGVGHGGEAGLLLGVDGGGTRTDCLLATLDGVVIG
                     RGSSGPSNLQAVGIDAAVCEIEKAVRLAMREAACSGGTARITSACFGLAGAGRKRDRD
                     MVEMRLRRLLAEVCSPDAPFPALTVVNDGIIALAGANAGEPGVVVIAGTGSLAYGVSP
                     DGREARAGGWGYILGDEGSAYDIGVKGLREALRAADGRQIPTCLTEDLAVALEIEEIE
                     DVVSVVYSGMTRPEIAALAPVVAKAAEKGDHVAQDIVVDAGRELGLMALAVIDRLGMS
                     GGAGNVAAVGGVLESNEMALDAMRGRVKVSAPAWSVERGKFSPAVGALLVAGRAIGGG
                     SIDEDHAPDNVCS"
     gene            2703..4346
                     /gene="nagZ"
                     /locus_tag="PLM83_01180"
     CDS             2703..4346
                     /gene="nagZ"
                     /locus_tag="PLM83_01180"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOK70057.1"
                     /translation="MELLDSMNIEEKIGQMVMVGLDGPELTSSDQEFIDKYKIGNVVF
                     LGRNIIDPKQAQSLTYGLQETASRRRHPIGFLLSIDQEGGVVARLTRGGTVFPGNMAL
                     GATRSEEYARRAAKVTADEMLALGFNMNLAPVIDVNSNPNNPGIGVRSFGEDVDMVTR
                     LGVATIEAYQASGVVATAKHFPGKGDVTVDSHLDLPTVPHNRERLERVEFAPFAASIR
                     AGVGAVMTAHVFFPAVEAEPDLPATLSRKVLTGLLREELGFGGVLLTDDLFMGAIAKR
                     FSIDEAAVRALEAGADIALLCHNQAEQAVAIEGIWDAVRSGRISEERVDESVRRILGL
                     KERFGLLAPERYLVRRSLKPVGSPENRRVALEIARDSVTLVANRQALIPMTPEDYPGL
                     VVMSPDIRGLTQVEDGQLAESPLAAAIKRFVPGVRDFGFSQSPDEREIAEAVSFASAK
                     DAVIIGTYNAHLYREQGRLVRALLEALDPNIPVVVVALRNPYDIQQFPSAETYIAAYG
                     FRECTMRAVAELIFGAIAPKGRLPVSIPGICACGAGLTL"
     gene            4366..5562
                     /locus_tag="PLM83_01185"
     CDS             4366..5562
                     /locus_tag="PLM83_01185"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOK70058.1"
                     /translation="MCSTMKVVGLMSGTSADGIDAALVEIEDRDSGLKAETLAFGNYH
                     YPERVREKIFELFSPKTSSADRICRFNFVLGRLFADAAVSVIEQAGLELGDVDLVAMH
                     GQTVCHLPWAGEGEFQVPSTLQIGEPAVVAERTGITVISNFRSRDIAAGGQGAPLVPY
                     ADYILFRHEDKTRVVQNIGGIANMTVLPRGARPQDVIAFDNGPGNMVIDAVVSEVTGG
                     CMDYDVDGAIAARGRVVEEFVAELMDHPFVRIEPPKSTGREEFGAEYVKSVMARARAL
                     GIGDEDIVASVTAFTAECIGKHLSDLVVPEYGLDELIIGGGGAYNPTLMRMIRERVDP
                     VPVVTHEAYGISSDAKEAIAFAILGYLGIHGIPANLPSATGAHHQVLLGAITPGRWSE
                     KVWDRL"
     gene            5598..6488
                     /locus_tag="PLM83_01190"
     CDS             5598..6488
                     /locus_tag="PLM83_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOK70059.1"
                     /translation="MTNDRDENRVQQTKNADYCLVTIRGRYPSLAPAEQKVAFFILEH
                     PESAAEMTSVQLAAAAGVSESTVVKCCQRLGFTGFSQFKLALVRELATSREGAFGEVE
                     PGDDVPAICDKIFTATLQAVEDTAKVLDPSEVARAAEAIHRARRLFFFGVGSSGVVAQ
                     DTQLKFMRIGMTAFSYLDTHAQLTQAALLNPEDVAVVITYSGRTREAMDVIDLARECG
                     ACTVCITNFPTSPAAVGSDIVLCTTAHEATGLRGGAMTSRLTQLAVIDCLFTAVAMKE
                     SDEAMELLMKTVDALASRKI"
     gene            6604..7857
                     /locus_tag="PLM83_01195"
     CDS             6604..7857
                     /locus_tag="PLM83_01195"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOK70060.1"
                     /translation="MINRAGLQQPEVEGWNVTGPVVKLGVTKLLEEPEKYLGTARVGL
                     VANYNAVDEYATPIIRLFAECPKIEFTRIYVAEHGLWGCEQAGVKVGHVGDLRTGVDV
                     VSLYGETVKPTPQMLEDIDVLVCDGNDIGSRYWTCLSTTALCIEAAAEAGKQIIVTDR
                     PNPIGGMAVEGNLLDIEYRSFVGYDRIPIRHGMTPGELAAMYVGGAGLDVDLLVIPMD
                     NWGREMLFPETGHFWTGTPNMPGFETALVYPGTCLFEGTTLSEGRGTTKPFRLIGAPW
                     LDAIDLADRLNTMELGGVRFRPAHFRPMFSKHSGVECAGVEVYVTDAWELDPVRLGVS
                     MLIAAREQNLDAFGWYGAGSPTGSADLARPFPVEGRHLFIDLLAGGAELRRWIEEGAT
                     SDEIMARWERETSEFMDAREPYLIY"
     gene            complement(8131..9093)
                     /locus_tag="PLM83_01200"
     CDS             complement(8131..9093)
                     /locus_tag="PLM83_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014277157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOK70061.1"
                     /translation="MTNHVLAGVMGLCVGDALGVPVEFTHRKTLKENPVVGMRAYGTY
                     NQPAGTWSDDTSMTLCLADSLIRGLDYRDIMERFLAWLNEGAYTPYGKAFDAGNTTKK
                     ALYKFSRGATPLECGGTDERDNGNGSLMRILPILFHLRSVYGPGFYDIDEAFDIIHNI
                     SALTHAHERSQIACGIYVSIASALMEGKDLKTHVETGIRRAMQYYIGQARFSHELHHY
                     ERLAHEGFNRLDEADIRSSGYVVDTLEAAVWCLLNTGSFEECVLKAVNLGEDTDTVAA
                     VAGGLAGLYYGYESIPGQWLSVIARREYVETLCNELYESFRNFL"
     gene            9757..10893
                     /locus_tag="PLM83_01205"
     CDS             9757..10893
                     /locus_tag="PLM83_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOK70062.1"
                     /translation="MGDRGARGVVVIVIALIVTAVSAMRFAPLGPEELEAYPSPSISR
                     VAKAKLSAYNSALLGTAGDTEVLIYEGSQPGGTMLVVGGTHPDEPAGTVAALILAENV
                     VVVQGKLIVIPYANASGFTHNLPQEGHPPYYTLSTPGGDRIMKYGSRLTNPVHQWPDP
                     TVYVEKVSYQKLAGTEARNLNRAYPGEKNGSLTERVAYAITRLIIDEEIDVSIDLHES
                     SPEYPVNNAVVAHERAFDLAAIATLDLEMEGVQINVEPSPPNLRGLSHREWGDNTDTL
                     ALLFESPNPVQGRLRGVTDADLIVDGVDPMYVKASERGRLYVPYTSEGTPLAMRAGRH
                     LASVQAIAAAYSMMAPDRAIVMAGLPSYGEIVANGAGAYLNPSR"
     gene            10914..11489
                     /locus_tag="PLM83_01210"
     CDS             10914..11489
                     /locus_tag="PLM83_01210"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOK70063.1"
                     /translation="MDGRMSNLRFVAVVVIAALVLTPVLAVAAPKPGKPALITSAGQS
                     TDGLILKTVMTDKATGETIPFEKLAKPEDLKGIRTLIVSVGLSSKGLGAAGVNVDQEK
                     ARVRSILDAAKKNDIFVIMVHIGGTARRGAGSDEFATLVADYAHHIFVVRAGNDDGFF
                     TRLAQKNKIPLTEVDDRIAIGPAIMKLLNTK"
     gene            11519..12562
                     /locus_tag="PLM83_01215"
     CDS             11519..12562
                     /locus_tag="PLM83_01215"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOK70064.1"
                     /translation="MRRMSRTVCAVFAAALVMAVAVAPALAEPIIHDVRPGYGVTGIG
                     WISDYHAPLRGSAVDTRVYYLDSKVEGPTTLVLGGTHSNEIAGIMAATLLVERAVVTT
                     GRLIVVPYANASGASYADTYHPEIGYYSLTTESGVRVFRYGDRLTNPEHQGPDADKYI
                     HYPSGQEFPGYEARNLDRAHPGKADGNLTQQLAYAYHQLLLEEKVDVAFDLHEAGPTS
                     RLANMVVANPKNIDFAVMAVVDLEMQGIVMNVEHSSDSFHGLSHREWGDTTPAASYLI
                     ETPNPAQAPNAINPDVVNDKVNPLSRRTATHLATIDAVLNAHELLDGKRTRWVGLPSY
                     EELVSDGLEEYLR"
     gene            12591..14312
                     /gene="ggt"
                     /locus_tag="PLM83_01220"
     CDS             12591..14312
                     /gene="ggt"
                     /locus_tag="PLM83_01220"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOK70065.1"
                     /translation="MFRPDSRKRIFAIVALLALVMVMTGVAGAELPQEVRAPNGMVAA
                     AHQLAAEAGVEILKAGGNAVDAGVATAFAVGVVEPNASGLGGEGMIVIYLPEGDRAVV
                     IDYRSTAPAAAAEALADSKMPGTGWPSVATPGLVAGLASALEQYGTMTLEQVLQPAIR
                     LAEEGFPIGATLAGVIEDRYEAVLNDPELAATFLDDGLPPQEGWILKNPGLASSLKKI
                     AAEGPDAFYRGEIAQAIDAASRAGGGYITAEDLANYKAVTRWPARGNYRGYEVFSAPP
                     PVGGATLIEVLNIVENFDLSAAPFPNAQAIHYLSESIKPAFRDYRAYVHDPDFVYVPL
                     QEMLSKDYAKERASKVNPDKMTPAGKIQAGEFAKKAALAPTGTEGAKYESPSTTHISV
                     IDKDRNMVAITQTISSFFGAGVMIEGTGVILNNEMANFSTDKTSGNYIAPNKRMRTSI
                     APTLVLREGEPFLSIGTPGAGRIVPTMAQLLVNIIDFDMGLQEAINAPRFYAREGSDV
                     FEYEPRFPEEVIDDLKKMGYPIDEKSVRGEYDLYFGGAQGVMVDPNTGELIGAADPRR
                     DGAAVGY"
     gene            14393..>15113
                     /locus_tag="PLM83_01225"
     CDS             14393..>15113
                     /locus_tag="PLM83_01225"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOK70066.1"
                     /translation="MWAQLITFVCMLGTFLLFAAALKLPPALATAGAAVVGLAVSGNI
                     GSLYHLIEGAFGYFDVILIIAMAMVFMAALEAAGILEEISYRITRQLGNKPLLLSLAS
                     MVLVMLPGMLTGSSTAAAMTTGRFVLPVLVNAGIAPARAGAFVALGSIIGMIAPPVNV
                     PVMIIGSGIDMPYVGFDIPLLLLSVPAAIAVSIWVVAGINKGPSGDKLEPGQAAPAGA
                     SERIPGFKVYIPIIALLAMMLA"
BASE COUNT         3336 a         3799 c         4658 g         3320 t
ORIGIN      
        1 aaactcttcg atgacgtggc ggcaatggat gagcgtgtcg cagaactttt gacccgggtg
       61 ggcttgtacc ccgaatacgc ccagagatat ccccatgagt tctccggagg ccagcgacag
      121 cggatcgcgg tggcgcgctc gatcgcggtc aatcctaggc ttctcatatg cgatgagccc
      181 acgtcggctc tggacgtctc cgttcagagc cagatcatca acttgctaaa ggagatacag
      241 cgggagacgg gcatgtctct catcttcatc tcccacaatc tggacgtggt ccatcacatg
      301 agcgacagga tcatggtgat gtaccttggc aatgttgtgg aatcagcgcc tgcctcggag
      361 cttttcgacg aaccggcgca cccctacacg agtgcgttga tgtcggccat ccccagctgg
      421 aatccgaaag accgcaagct ggctaaagtc aagcttgagg gggaaccgcc cagccccatc
      481 aatccgccgt cgggttgccc gttccatccg aggtgtccgc acgcgatgga gctttgtcat
      541 gagatcaagc ccgagccgca agaggtgaac gatggacacc tgtgcgcctg ccatctgcat
      601 gctcgggcgg atgcagaggt tgctgcgagg tagttgagtt aggcgcccca ggccacaagc
      661 tggcgggacc ggcgcggaaa gtggtggtgc ttgtggagaa tatggactac gctgatcttg
      721 ccacagagga aacgaacccc agatcccgtg ggctagaccg gatgagcaca gtgcagatcc
      781 ttgaggttat gaacgaggag gatcggcgcg tgcccgaggt cgtagccagg gctattccgg
      841 agatcgcaaa ggctgttgac atgatcgtgg cgcgtatggc cgcaggcgga aggctcctgt
      901 acgtgggcgc cgggactagc gggcgcctgg ggatcctgga tgccgtggaa tgcgtcccca
      961 cgttcggagt agatccaaac caggtttctg ccgtgatcgc cggcggacct ggagccgtat
     1021 tccattctgt tgaagccgca gaggatgacg agtcgtcagg ggttcgcgat gttgcggagt
     1081 caatcacgga attggatgtt gtggttggaa tatccgcaag cggcgttacc ccttacgttc
     1141 agggagcatt gaaagcggcg aaagaagtcg gcgcgggcac tgtggcgatc gtgtgcaacc
     1201 acgccgataa gctggacctt gatgtagatg ttgttatatc cctgcccgtc ggcgctgaag
     1261 tgctcacagg ctccacccgc ctcaaagccg gaaccgccca gaagctcgtc cttaacatga
     1321 tatccactgc cacaatgatc aagctgggca aggtgtacga caacctcatg gtggacatgc
     1381 aggcgaccaa caggaaactg aagcgtcgtg ctgttagaat cgtatccatc gccacggggc
     1441 tggatgaaga ggtcagcgag gatttgctgg ataaggcggg cgggaacatc aagaccgcca
     1501 tcgttatggc gttggcatca gtcaataggc gcgaggccga gcgtgctctg gagaaggcgt
     1561 gtgggcatac cagaaatgcg gtacaggcgg ccatagagcg cacgaggtga ggagaaatgc
     1621 ctgctgaacg atttcctcct ttactaggcg ttggacacgg aggcgaagcc ggactccttt
     1681 tgggtgtaga cggcggcggg acgaggacag actgtctgct cgccaccctt gacggcgttg
     1741 taataggccg aggatcctct ggcccttcca accttcaagc ggtcggaatt gatgcggcgg
     1801 tttgtgagat agaaaaggcc gtcagattgg ccatgcgcga ggcggcatgt tccggcggga
     1861 cggcgagaat cacttcggca tgcttcggcc tagcgggcgc cggccgtaag cgcgaccgcg
     1921 acatggttga aatgaggctt cgtcggctgc ttgcagaagt ctgttccccg gatgcgccgt
     1981 ttcctgcgct caccgtggtc aacgatggga tcattgcgct tgccggggcg aatgcgggcg
     2041 agcccggcgt agtagtgatc gcggggaccg gatccttggc gtatggcgtg tcgcctgatg
     2101 gccgggaggc gcgagcaggg ggatggggat acattctcgg ggatgaaggt tctgcatacg
     2161 atattggcgt taaagggctc cgtgaggcgc tccgggcggc ggacgggagg cagattccca
     2221 catgtttgac ggaagacctc gctgttgcgc tcgagatcga agagattgaa gatgttgttt
     2281 cagtggtgta ttcggggatg acgagaccgg agatcgcggc gcttgcccct gtagttgcca
     2341 aggcggccga aaagggagat catgtcgcac aggacatagt tgtcgatgcg ggccgagaac
     2401 ttggactgat ggccttagcg gtaatcgatc gactcgggat gagcggcggc gctggcaacg
     2461 ttgcagccgt cggcggcgtg ctcgagtcca acgagatggc gcttgacgcc atgcgaggca
     2521 gggtgaaggt atcggcgccc gcgtggtctg ttgagcgagg caaattttcg ccggcggttg
     2581 gcgccctgct tgtcgccgga cgggcgattg ggggcggctc gatcgacgag gaccacgcgc
     2641 cagataacgt ctgtagttga tggggcagtt gggtggctgg gtgatggaaa ggcgggggta
     2701 gaatggaact gcttgattcc atgaacattg aagagaaaat agggcagatg gtaatggtgg
     2761 gtctggatgg ccccgagctt acgtcatctg atcaggaatt tatcgacaaa tacaagatcg
     2821 gcaacgtagt cttcctcgga aggaacataa tcgatccgaa gcaggcacag tctctcacct
     2881 acgggctcca ggagaccgcc tccaggcgcc gccatccaat aggatttctt ctctccatcg
     2941 accaggaggg cggggtggtt gcccggctga caaggggcgg gactgtgttt cctgggaaca
     3001 tggccctcgg ggcgacccgg tcagaggagt acgcacgccg ggcagccaag gtgacggcgg
     3061 acgagatgct tgccttggga tttaacatga acctcgcgcc ggtaatcgat gttaacagca
     3121 atcccaacaa ccctgggata ggggtccgct cattcgggga ggacgtcgat atggtgaccc
     3181 ggctcggagt tgcgactatc gaggcttacc aggcctcggg ggtggtagcc actgcaaagc
     3241 attttccggg gaagggcgat gtcacagtag attcccacct cgacctgcct accgttcccc
     3301 ataacagaga gaggttggag agggtggagt ttgccccgtt tgcagcttcc atacgagcag
     3361 gggttggggc cgtcatgacc gcccacgtat ttttcccggc cgtggaggcg gaacctgacc
     3421 ttccggcgac tctttcccga aaagtgctca cgggcctgct cagggaagag ctggggtttg
     3481 gcggagtgct gctcacagac gacctcttca tgggcgccat cgcgaaaagg ttttccatcg
     3541 acgaggcggc agtgcgggcg ctggaggctg gggcggatat cgctctgctc tgtcacaatc
     3601 aggccgagca ggccgtggcg attgagggca tatgggatgc tgtgaggagt ggaaggatct
     3661 cagaggagag ggtggatgaa tcggtcaggc gaattttggg gctcaaggaa cgtttcggct
     3721 tgcttgctcc agagagatac ctcgtccgac gcagcctgaa gccggtagga agcccggaaa
     3781 atcgtcgtgt tgccctggag atcgcaaggg attcggtgac ccttgttgcg aatagacagg
     3841 cactcattcc catgacgccg gaggattacc cgggccttgt agtcatgtct cccgacatcc
     3901 gagggctgac acaggtcgaa gatggtcaac tggcggaatc tccgcttgct gcagcgatca
     3961 agcgctttgt tcctggggtt cgggatttcg gtttttccca gtcccccgat gaacgggaaa
     4021 tagcggaggc ggtcagtttt gcgagcgcca aagatgcggt gattatcggc acatacaacg
     4081 cccacctcta ccgagagcaa ggcagactgg tcagggcttt gctggaggcg ctcgatccaa
     4141 acatccccgt ggtggttgtg gcgctcagga atccatacga cattcaacag ttcccgtctg
     4201 cagagacgta catagccgcc tacgggttca gggagtgcac gatgcgagcc gtggcggaac
     4261 ttatcttcgg cgcgatcgcg cccaaaggga ggctgcccgt ttccatcccg gggatatgcg
     4321 cttgcggggc cgggttgact ctatagggag gagagtcggg tcgctttgtg ttcaacgatg
     4381 aaggttgtgg gccttatgtc aggcacatcc gctgacggca tagatgctgc cctagtggag
     4441 atagaggatc gggattcggg cctgaaagcg gagacgttgg cgtttggcaa ttaccactat
     4501 cctgagagag tgagagagaa gatattcgag cttttctccc ccaaaacgtc ttcagcggat
     4561 agaatatgca ggttcaactt cgtgctgggc aggctctttg cagatgctgc ggtttctgtg
     4621 atagagcagg cgggcttgga attaggagat gtagatctcg tggccatgca cggccagacc
     4681 gtatgccatc tgccctgggc aggagaaggg gagttccaag tcccttcaac gctccaaata
     4741 ggcgagcccg cggtagtggc cgagcgcact ggcataaccg tcatctcgaa cttcaggtcg
     4801 cgcgatattg cagcaggcgg ccagggggct ccattggtac cttatgccga ttacatcctc
     4861 tttcgccatg aggacaagac gagggttgtg cagaatatag gggggattgc caatatgacg
     4921 gtcctgcccc gcggcgcgcg gcctcaggat gtgatcgctt tcgacaatgg gccggggaac
     4981 atggtgatag atgcggtggt gagcgaagtc actgggggat gcatggatta cgatgtggac
     5041 ggggctattg cggcaagggg cagagtggta gaggaattcg tggcggagct gatggatcat
     5101 ccttttgtgc ggattgagcc tcccaagagc acggggagag aggaatttgg cgcggagtat
     5161 gtaaagagtg tcatggctcg ggcgcgggcc ttaggaatcg gcgacgagga cattgtcgcg
     5221 tctgtcacgg cttttaccgc tgaatgcatc ggcaagcatc tgagcgactt ggtggtgcct
     5281 gaatatgggt tggacgagct gatcatcggg ggaggcggcg cctacaatcc caccttgatg
     5341 aggatgatca gggagcgagt ggatcctgtg cctgttgtaa ctcacgaggc gtatgggata
     5401 tcgagcgatg ccaaagaggc catcgctttt gcgatcctcg gatatctcgg catacatggg
     5461 atcccggcga atctgcctag cgcgaccggc gcccatcacc aggttctgct cggagccata
     5521 acgcccgggc gctggtcgga gaaggtatgg gatcggcttt aggctacaca aggatacttt
     5581 gagggggcag gagacagatg acaaatgaca gagatgaaaa cagggtgcag cagactaaga
     5641 atgccgacta ctgccttgta actatccgcg gacgatatcc ttctcttgct cctgccgagc
     5701 aaaaagtcgc attcttcatc ctcgagcacc ctgaatccgc cgccgagatg acctcggttc
     5761 agctggctgc agctgcagga gtgagcgagt cgactgtggt gaaatgctgc caacgccttg
     5821 gattcacagg attttcccaa ttcaagttgg cgttggtcag agaactcgct accagcaggg
     5881 agggtgcgtt cggcgaagtt gagcctggag acgatgtacc cgccatctgc gacaagatat
     5941 tcacggcaac gcttcaggcg gttgaagata cagctaaggt gcttgacccc tccgaggtcg
     6001 cccgcgcagc agaggccata catcgtgcca gacggttgtt cttcttcggc gtaggctcct
     6061 caggggtggt tgcccaagac acccagctca agttcatgcg catagggatg acagccttca
     6121 gctatcttga tacccatgct cagcttacac aggctgctct gctgaatcca gaggatgtgg
     6181 cggtggtgat aacctactca ggccggacga gggaggccat ggatgtcatc gatcttgcgc
     6241 gggaatgcgg ggcgtgcacg gtctgcatca cgaattttcc gacttcgcca gcggctgtgg
     6301 gctctgatat cgtgctctgc accacggcac acgaggccac tgggctcagg ggaggggcga
     6361 tgacctctcg gctgacacag cttgccgtga tcgactgcct tttcaccgca gtggccatga
     6421 aagaatcaga cgaggcaatg gagctcctca tgaaaaccgt ggatgccctg gcatcaagga
     6481 agatctagat ggctttcgag tggtgttcgg aaggtgggga tattggcccc tgggtacgtt
     6541 tttcactgcc tgccagagaa ccggggattg gtgtgaagtg atggggctag gaggatctgt
     6601 ggcgtgatca atcgagccgg actgcagcag cccgaagtgg aggggtggaa cgtgactggg
     6661 cccgtggtca aactgggtgt gacgaagctc ctcgaagagc cggagaagta tctcggcacg
     6721 gcgagagtgg gccttgtggc caactacaac gcggtggatg agtatgcaac cccgataatc
     6781 aggctctttg ccgagtgccc gaagatagag ttcacccgga tttacgtggc tgagcatggc
     6841 ttgtggggct gtgaacaggc cggggtgaag gtaggacatg tcggtgattt gcgaaccggc
     6901 gtagatgtgg tcagtctgta cggcgaaacg gtgaagccga ctccgcaaat gctggaagat
     6961 atcgatgtgt tggtctgcga cggcaatgat attggatcgc gctattggac ttgcctttcc
     7021 accacggcac tctgtattga ggcggctgcc gaagcgggaa agcagataat cgtgacggac
     7081 aggcccaatc ccatcggcgg aatggctgtt gaaggcaatc ttctcgacat cgagtaccgc
     7141 tcttttgtgg ggtacgatcg gatacctatt cgccatggaa tgaccccggg ggaactggcc
     7201 gcgatgtacg tcggcggggc aggccttgac gtggatcttc tcgtgattcc catggataac
     7261 tggggaaggg agatgctctt ccccgaaacc ggccattttt ggaccggcac gccaaacatg
     7321 cccggttttg agaccgccct cgtgtacccc ggaacctgct tgtttgaggg aaccacgctt
     7381 tccgaaggaa ggggaacgac gaagccattc aggctgatcg gagcgccctg gctcgatgct
     7441 atcgatcttg ccgatagact caacaccatg gagcttggcg gcgtacgctt taggccggcc
     7501 cacttcaggc ccatgttttc aaagcacagc ggcgtggagt gtgctggggt agaagtttat
     7561 gtgacagatg cttgggagct tgatccggtt cgcttggggg tttcaatgct gatagccgca
     7621 agggagcaga atctggacgc ttttggatgg tatggtgcgg gaagtccgac cggctctgct
     7681 gacctggcgc gcccgttccc ggtcgaaggg cggcatctgt ttatcgatct tcttgcagga
     7741 ggcgccgagt tgagaagatg gattgaggag ggcgcgactt ctgatgagat aatggccagg
     7801 tgggaacggg agacatccga gttcatggat gcgcgggagc catatctcat ttattagcag
     7861 tggtagagca agctcatgct ttagccccta aacgcgctgt ttgccgcctg catgcggctg
     7921 attctttaaa ggatggctat acatgaaaaa tgcccctcca agtctagtgc cgcggaatat
     7981 gtttggaact agatagcacc cgatatttcc cgctccccta tttagcggag ctcgctgcta
     8041 tcggcaactt tgttgccggc tcgggcacct atgccacatc ccaaaggtga ggtatacgtg
     8101 cgcgtcagaa cgtctgacag gcaatgccgt ttacaagaag ttgcgaaatg attcgtaaag
     8161 ttcgttgcag agagtctcaa catattcccg ccttgcgatc actgaaagcc attgacccgg
     8221 aatgctttcg tacccgtaat aaagccctgc aagccctccg gcaactgcag cgactgtgtc
     8281 ggtatcttcg cctaggttta ctgccttcag aacacactcc tcgaaactgc ctgtgttgag
     8341 cagacaccaa acggcggctt cgagcgtatc gacgacgtat ccgctgcttc tgatgtcggc
     8401 ttcatccagt cgattgaagc cttcatgcgc aagcctttcg tagtgatgga gctcatggga
     8461 aaatcgggct tgccctatgt agtattgcat ggcccttctg atccccgttt cgacgtgtgt
     8521 tttgagatcc ttcccttcca tgagagctga agcgatagat acatagatgc cgcatgcgat
     8581 ctggctgcgc tcgtgggcat gggtgagggc tgaaatgttg tggatgatgt caaaagcctc
     8641 atctatgtcg taaaagccag gcccatagac agagcgtagg tgaaatagga ttggaaggat
     8701 cctcattagc gagccgtttc cgttgtcgcg ttcatctgta cctccgcact ccaaaggggt
     8761 tgctccccgg gagaacttgt acagggcttt tttagttgtg ttccccgcat cgaaggcctt
     8821 tccgtatggc gtatacgccc cttcattgag ccatgcgagg aatctctcca tgatatcccg
     8881 atagtccaat cccctgatga ggctgtcggc aaggcatagc gtcatgcttg tatcatcaga
     8941 ccaagtcccg gccggctggt tatatgtgcc gtaggcgcgc ataccgacca ccgggttttc
     9001 cttcagagtc tttctgtgag taaattccac gggaacgccc agcgcatcgc cgacgcatag
     9061 tcccattact cctgccaaca catgattcgt catgcataca ctcctcatat acgtcagtag
     9121 tcaagccgtc gcttctgtgc gaatctctcg aagttcgcct cccagatgcg actatgccaa
     9181 gcatcatcct acagttcaat tgagcgataa tccgtttggg catagattcg ttgcgcctgc
     9241 gagaggctcc tcttaggcct gttccaaatc tttgcggctt tggatttcga ttgtctcatg
     9301 tgaaagtgta attaaaatct gattggtggt gcaagtttgg agcaggatgg ggatgaatga
     9361 aaaagaggcc gcgtggtcac ggaagatgtt cctgagcggg acatcgctgt tagggcatct
     9421 ttatcgataa gctttgtggc atttctgcag gggagtgtgt agattttacg aaaacagatt
     9481 attcagggag tcctggcgac gccattaaga ggtaattggt aagggttttt tgggtttata
     9541 tatgcaaaat ggagtttatg aggccaacat gtagaagagg agacataatg cgaaacttgt
     9601 atggaaatgg gcagctcaac aatgcattgt attaatatgg tggtattgtg aaggcaaaac
     9661 caaaggagga aaaccatatt ccgacgtaga ataaatatgg aacaacgaat atggagcgcg
     9721 caaagcctgt tgactatgga tacggaggtg aaacccatgg gcgacagagg cgcaagaggg
     9781 gtagtcgtaa tcgtcattgc tttgattgtg acagcggtat ctgcaatgag gtttgcgcca
     9841 ttgggccctg aagaactaga ggcgtaccca tcgccttcaa tcagtcgtgt agctaaagct
     9901 aagctatctg catataactc ggcacttttg ggcactgcag gagatactga ggtattgatc
     9961 tatgagggct ctcagccagg cggaacgatg cttgtggttg ggggaactca tcctgatgag
    10021 ccagcgggca cggttgcagc tctgattctg gcagagaacg ttgtcgtggt gcagggcaag
    10081 ctgattgtga taccttatgc aaatgcttca ggctttaccc acaaccttcc tcaggaggga
    10141 catcccccat actacaccct atctaccccg ggtggcgatc gaatcatgaa atatggttcg
    10201 cgtttaacga atcccgtcca ccaatggcct gatcccacgg tctatgttga aaaggtatcc
    10261 tatcagaaac ttgccggaac cgaggcacgc aatctcaaca gggcttaccc cggcgaaaaa
    10321 aacggctcgc tgacggaacg cgtggcatat gccatcaccc ggctcatcat cgatgaagag
    10381 atagatgtct ccatcgacct ccacgaatca tctcccgagt atcctgtgaa caatgctgtt
    10441 gttgcccacg agcgggcgtt tgacctggca gctattgcta ctctcgattt ggaaatggaa
    10501 ggcgtgcaga tcaacgtcga gccgtctcca cccaacctga gggggctttc gcacagggaa
    10561 tggggagata ataccgatac gttggcgctg ctgtttgaat cacctaaccc cgtccaaggc
    10621 agactgaggg gagtaacaga tgctgatttg atcgttgacg gggtggatcc tatgtacgtg
    10681 aaggcatctg agcgaggacg tttgtatgtg ccatatacaa gtgaaggaac tccgcttgca
    10741 atgagggcgg gcaggcacct tgcgtcagtg caagctattg cagcggccta ttccatgatg
    10801 gcgcctgaca gagccattgt tatggcgggg ttgccgtcgt atggcgagat agttgcaaat
    10861 ggcgcaggag cctatctgaa cccaagcaga taaagaagga aggagaacaa cacatggatg
    10921 ggagaatgag caatcttcgt tttgtggctg tagttgttat cgcggcactc gtgctgactc
    10981 ctgtacttgc agttgctgcg cccaaacccg gcaaaccggc gctaataaca tctgcagggc
    11041 agagcactga cggtctgatt ctgaaaacag ttatgaccga caaggctaca ggcgagacaa
    11101 ttcctttcga gaaacttgca aagcccgaag atctcaaagg aattcgaact ctgattgtgt
    11161 cagtcggcct aagcagcaag ggccttgggg ccgcaggggt caacgtcgat caggagaagg
    11221 ctagagtccg ttcgattctt gatgcggcta agaagaacga catattcgtg attatggtgc
    11281 acataggtgg caccgctcgt cgtggcgcag ggtctgacga attcgcaaca ctagttgccg
    11341 attatgcaca ccacattttc gtggtccggg cgggaaacga tgacggattc ttcacccggt
    11401 tagcgcagaa aaacaagatt cccctgacag aagtggatga caggattgcc atagggcccg
    11461 caatcatgaa actgcttaac accaagtaac atgagagcat gtgtctggag ggtattagat
    11521 gcgcagaatg agtaggacgg tctgtgcagt tttcgcggcg gctctggtga tggcagtggc
    11581 tgtagcgcct gctctggctg agccgataat tcacgatgtg aggcctggat atggggttac
    11641 gggaatcgga tggatatccg attaccacgc cccgttgcgc ggttctgctg tggatacccg
    11701 tgtatactat ctggacagca aagtggaagg gcccacgacc cttgtgttgg gaggaactca
    11761 ttccaacgag attgccggga tcatggctgc cacattgctc gtggagaggg cggtggttac
    11821 taccggaaga ctcatagtag tcccatacgc caacgcatcc ggggcaagct atgcagatac
    11881 atatcatccc gagatcggtt attacagctt gactacagag agtggagttc gtgttttcag
    11941 gtatggagat cgcctcacaa acccggagca ccagggaccc gatgctgaca agtacattca
    12001 ctatccgtcg ggtcaggagt ttcccggtta tgaagcgcgc aaccttgaca gggcccatcc
    12061 gggcaaggct gacggcaacc tcacccagca gcttgcatac gcttaccacc agttgttgct
    12121 tgaggaaaag gtagatgtgg ctttcgacct gcatgaagcc ggccccacgt ctcgactggc
    12181 gaacatggtt gtggcaaacc ccaagaatat tgatttcgcg gtgatggccg tagtcgatct
    12241 ggagatgcag ggaattgtga tgaatgtgga gcattcgtcc gacagttttc acggtctttc
    12301 ccacagggag tggggagaca ctactcctgc tgcttcgtat ttgatcgaaa cgcctaaccc
    12361 tgcccaagcc cccaatgcca tcaaccctga tgtggtaaat gacaaggtta atcccttgtc
    12421 ccgcagaact gctacacacc ttgctacgat agatgcagtg ctcaatgcac acgagctcct
    12481 tgacggcaag cgcacgcggt gggttggatt gccttcttat gaggaattgg tttcggacgg
    12541 actcgaagaa tatctgagat agaccggaat taagaaggag ggcacagact atgttcagac
    12601 ctgattccag gaaacgcatt tttgcaatag tggcactgct tgcgttggtt atggttatga
    12661 ctggtgtggc aggggcggag ctgccacagg aagtccgcgc gccaaacggt atggttgctg
    12721 cggcgcacca gttggcggcc gaagccggag ttgaaatcct caaagctggt ggaaacgctg
    12781 ttgatgctgg agttgccact gcgttcgcag tcggagtagt ggagcccaac gcatcaggct
    12841 taggcggcga gggcatgatc gtaatctacc ttcctgaggg agacagggcc gttgtgatcg
    12901 actacaggtc gacggcgcct gcggctgcgg ctgaagccct ggcagacagc aaaatgccag
    12961 ggacgggctg gccgtcagtg gccacccccg ggctcgttgc gggcctggct tcggctctgg
    13021 agcagtacgg gacaatgacg cttgagcagg tattgcagcc tgcaattagg ctggctgaag
    13081 agggattccc tattggcgca accctcgccg gcgtcattga agaccgatac gaggccgtac
    13141 tgaatgaccc tgagcttgct gcgacattcc ttgacgatgg tctgcctccg caggagggct
    13201 ggattctgaa aaaccccggt ctcgcgtcat cgctcaagaa gatcgctgca gaaggccccg
    13261 acgcattcta tcgcggggag attgcgcagg cgatcgatgc tgccagccga gcgggcggcg
    13321 gctacatcac cgctgaagac ttggcaaatt acaaggccgt aacgagatgg cctgcacgag
    13381 gcaactacag aggatacgag gtgttctctg cgccgccacc ggtaggaggc gcaaccttga
    13441 ttgaagttct gaatattgtg gagaacttcg atctcagcgc agctccgttc ccgaacgccc
    13501 aggcgattca ctacctttcg gagtcaataa agcctgcgtt ccgtgattac cgtgcatacg
    13561 tgcatgatcc tgatttcgtg tatgtgcctt tgcaggagat gctctctaag gattatgcaa
    13621 aagagcgcgc ttccaaggtc aaccccgata agatgacccc agctggcaag attcaggctg
    13681 gcgaatttgc caagaaggct gctctggctc caactggcac cgaaggcgcc aaatacgaat
    13741 caccgtcaac aactcacatt tcagtaattg acaaagaccg aaacatggtg gcgatcaccc
    13801 agactatcag ctcattcttc ggagcaggcg taatgattga gggcactggt gtgattctca
    13861 acaacgagat ggccaacttc tcaactgaca agaccagtgg caactacatt gctcccaaca
    13921 agaggatgag gacttcgatc gctccaacgc tcgtgctgcg cgagggagag cccttcctga
    13981 gcatcggaac tccgggcgcc ggaaggatcg tgcctacaat ggcgcagctg ctggtcaaca
    14041 taatcgactt cgacatgggg ctgcaagagg ccataaatgc acccagattc tatgcgcgag
    14101 agggttctga cgtgttcgaa tacgaacccc gcttccctga agaggttatt gatgatctga
    14161 agaagatggg ttatcccatc gacgagaagt cggtacgagg tgaatacgat ctgtatttcg
    14221 gaggcgctca gggcgtcatg gtcgacccca acaccggcga actgattgga gctgctgacc
    14281 caaggcggga tggcgctgct gtaggttact gaggacataa ggaagcaaac aaaaccgggc
    14341 gcggcggtcg ggacgccgaa cgtcgcgcct ttttcatgga agagtgatgc taatgtgggc
    14401 tcagctcatt acatttgtct gcatgctagg cacgtttttg ctgtttgctg cagcgctcaa
    14461 gctgcctcca gccctggcta cagcgggcgc cgctgtagtg ggccttgctg tttcgggcaa
    14521 catagggtcc ttgtatcatc tgattgaagg ggctttcggc tacttcgatg tgattctgat
    14581 tatcgccatg gcaatggtgt tcatggccgc gctcgaggca gcaggaatcc tggaagagat
    14641 ctcctacaga atcaccagac agctcggcaa caagcctctg ttgctctctt tggcgtccat
    14701 ggtcttggtg atgcttccgg gcatgctgac cggttcatcc acagctgccg ctatgaccac
    14761 aggaaggttt gtcttgcctg tgctcgtcaa tgctggcatt gctccggctc gggcaggcgc
    14821 atttgtcgct ctcggcagca taatcgggat gattgcgcct cctgtgaacg tgccggtgat
    14881 gattatcggg tcgggaatag acatgcccta cgtgggcttc gacatcccgt tgttgctcct
    14941 ttccgtgccc gccgcgatag ctgtctcaat atgggtggtg gcagggatta acaaggggcc
    15001 ctcaggcgac aagctagaac cgggacaggc cgcgccggca ggcgccagtg aacgcattcc
    15061 cggtttcaaa gtttacattc ccattatcgc acttttggcg atgatgctcg cgg
//
LOCUS       DAOSAI010000014        14667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_2782_length_14667_cov_4.194429, whole genome shotgun sequence.
ACCESSION   DAOSAI010000014 DAOSAI010000000
VERSION     DAOSAI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14667
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2782_length_14667_cov_4.194429"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /gene="ligA"
                     /locus_tag="PLM83_01230"
     CDS             complement(<1..1013)
                     /gene="ligA"
                     /locus_tag="PLM83_01230"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOK70067.1"
                     /translation="MTDAASRVDELRELIRHHNWRYYVLDDPEITDAEYDMLMRELIA
                     LEEAHPELADPDSPTQRVGGAVSAGFEPVVHAIPMLSLDNMFDEGSLRAFDARIKRAT
                     GLAKVEYVAEPKIDGLAISLTYENGRLAVGATRGDGERGEDVTHNLRTIRAVPLVLPK
                     GAPDSVTVRGEVYMRKSEFERLNQERASRGEPMFANPRNAAAGSLRQLDPKVTASRKL
                     DAFFYYLPEAERYGIETHWDALDFIRSLGFRVNPEIILCDGLGETLEFIHRLADLRPM
                     LPYEIDGVVVKVNRLDLQRKLGFTSHSPRWASAYKYPAQQVQTRVRDIIVQVGRTGVL
                     TPTA"
     gene            complement(1035..3299)
                     /gene="pcrA"
                     /locus_tag="PLM83_01235"
     CDS             complement(1035..3299)
                     /gene="pcrA"
                     /locus_tag="PLM83_01235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HOK70068.1"
                     /translation="MGGDLLGALNEPQREAVVCVDGPLLILAGAGSGKTRVLTHRAAY
                     LISERRVPPYRILAVTFTNKAAKEMKNRVEELVGARADLMWISTFHSLCARMLREDIP
                     SLGYKKSFTILDESDQTAVVKKCLDDLNISGDMFKPQAVLAAISSAKNELIGPEELEA
                     NARDPRTRTMGQIYAAYQSTLVRGNALDFDDLITLAVRLLRECPEILEKWQDRFEYLM
                     VDEYQDTNHAQYILVKLLAARSRNLCVVGDDDQSIYEWRGADIRNILDFERDYPEARV
                     IKLEQNYRSTQNILNAANCVIANNKGRKDKSLWTSRGSGSPIFVYCAENERDEGRFIA
                     DEIQRLAALEGRAYRAFAVLYRVNAQSRAIEEVLLNRGMPYKIVGGLKFYERREIKDV
                     LAYLRVIENPEDSLALERIINVPRRGIGDATLAIVRRLSEEAGVPLYAGVELAVRASD
                     TPSRARSGLSQFLQLMEGLQSRKGEATVSQLVEMVLEKTGYIRELEREQTLEAEGRIE
                     NLRELLTVTQEFEARRMENSVEDGGGPTDIGAFLEEVSLLTDLDRADLSDDSITLMTM
                     HAAKGLEFPVVFIAGMEENIFPLARSAREERSLEEERRLCYVGITRAKDRLYLVHSVM
                     RMIYGSTMANAPSRFLGEIPKELLADAMHDRRSLWGRSDGPASPVIRAHSVEGRSGTA
                     WNRGDSSDGGGENHGGDGSAEYSAGDRVRHAKFGEGTVVAVARGGNDWVITVAFPGGG
                     IKHLAQSFAPMERI"
     gene            complement(3304..4782)
                     /gene="gltX"
                     /locus_tag="PLM83_01240"
     CDS             complement(3304..4782)
                     /gene="gltX"
                     /locus_tag="PLM83_01240"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOK70069.1"
                     /translation="MNADSTSSKDRKVRVRIAPSPTGNCHVGTARTALYNMLFARKYG
                     GTFVLRIDNTDLERSTKESEEGVLEGLRWLGIKWDEGPDIGGPYGPYRQLERMDTYKE
                     TVEKLLSEGKAYYCYCTPSELEEERKAALAAGKPPRYSGKCRELSSADRARLEAEGRR
                     PVVRLRVPDVTLSFDDLIRGHQQKDMSERGDFIIFKSDGTPVYNFATVVDEHYMEMSH
                     VFRAVEHLTNTFDQVCVYTAMGWEPPQFGHLTLMLNPDRTKISKRAGAVYIGEFREMG
                     YLPEAVINFLALLGWNPGTEQEIFSIDELVEAFSVDTLSKSDAVFDMKKLDWFNGVYI
                     RSLTVPDLASRVRPFMEAAGYSVDDTDYLERAVALITDRIGKLTEAPDLLGFLFVDDV
                     ELDPSALKTKAKFSYDDIARGLKASADALEKVSTWEHEPIEEALRGTATELGWKAGEL
                     LMPVRIAVTGRRVTPPLFESLVLLGRDRSLARIRKAIALLEE"
     gene            complement(4807..6162)
                     /locus_tag="PLM83_01245"
     CDS             complement(4807..6162)
                     /locus_tag="PLM83_01245"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOK70070.1"
                     /translation="MVGQERAQEIVEAAISACRADAVEAVLMSDVDNLTRFANSEIHQ
                     NASVSSDILYVRAICGKREGWATTNQLTSEAVRDAAALAVEIAMVQPENPDFPGLAGL
                     CRYEEAGGYDERTASFSARERAMAVKAITAIADKHELNASGAFRTRVTGMAIANSEGA
                     SAWMRGTDSAISAVIMTGYDSNAGSGYAEAASWSAGNLDARAIGERAVEKCLSSRNPG
                     ALEPGEYEAILEPAAVVDMMETLALMGINGISFIEGRSYASGRIGSKITSDMVTIEDN
                     WHDPGLLPRPFDLEGVPRRPVTLIEHGVASGMIFDRLAAKKAGTESTGHALVGGALKG
                     GYPLHLRMSPGESSIGEMVASTGRGVYVTRFNYTNVVHPTKTIFTGMTRDGTYLIEDG
                     RIVRPLRNLRFTDSILDGILANIVAVSRDTELLPVAGGRGIYRAPAIKTAKLNFIGTT
                     A"
     gene            complement(6156..7601)
                     /locus_tag="PLM83_01250"
     CDS             complement(6156..7601)
                     /locus_tag="PLM83_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOK70071.1"
                     /translation="MRVLEIALDRACATGAEYADVRALHQTEERLSVRNGVVERATSG
                     VSHGIGVRARVDGAWGFAASCVVSESEAARVAAEAVEIARASAMAKKCDVVLAPLDPV
                     VVEWESPCAIDPWSVPRAQKIDLLREACSRAMSGGSDVRYASASMNAIRDDKAYANSE
                     GARIRQRTTVTSAGIKAIALGNGEMQVRSYPGTFEGNVRQAGYEWIEEMDIAGHAAEI
                     GKQAQALLAAQQCPQIKTTLILGTNQLALQIHESCGHPTELDRVYGYEASYAGTSFLD
                     PGKLGDFRYGSDLVNIYADSTIPGGLGTFAYDDEGVRSCRTDLVRNGMFEAYLMSRET
                     AARLGKTSNGCMRASGWDRMPIIRMTNICLEPGNETLESLIRGTDDGIFMDGIKSWSI
                     DDRRLNFQFGSEIGWRIRNGQLAEMVKNPTYTGITPEFWNSCDGIAGSDEWVLWGFPN
                     CGKGEPSQVMFVGHGTAPARFRNVKVGVGRW"
     gene            complement(7606..8496)
                     /locus_tag="PLM83_01255"
     CDS             complement(7606..8496)
                     /locus_tag="PLM83_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOK70072.1"
                     /translation="MKSWRLGSYKSILADLSLVGICVIWGTSFAIVKTVLGDTNPATF
                     IAARFVLATVLLGLVAGRRLRGLDWEYCKAGFALAIPLMLGFLTQTRGLQGTTASKAG
                     FITGMHVVFTLFLEQKVLKNRIMPYQITGVGVAVAGLGLLTLDRLSPPTYGDILILVC
                     AFCFAVHIILLARYSPTMDGLLLSFTQMAWAAVFSVLAAGSDIVQIFQFDKDLVLSVA
                     YLGATGTALAYLVQTTAQKYTPPTRAALILQTEPIFTSIFARLWLGERLSVQQAIGAI
                     LILAGILLCQVGDIKRSREV"
     gene            complement(8841..9779)
                     /locus_tag="PLM83_01260"
     CDS             complement(8841..9779)
                     /locus_tag="PLM83_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70073.1"
                     /translation="MGRAIAGEPRQDAEERVRRHILGQLHSHEIDRFELIRSKDRVWV
                     FRCSFDGSPTVIKYFEKDDDLREIHNYQLLHRLGIPTMDVLEYAQRCLVLEDIAESKT
                     WRLGIPDDLRDEQIAGRLAEWYFHFHEKGINIPELPELYCETDCLTIANLNRLSERDP
                     SLKDITCYLQNHVGLFQKELAGLVRTLNYNDFYWTNMLVRKDHTAAMMFDYNYLGQGY
                     RGNDISNVKSSLSVEAGTAFEDQYDMLYHGKYGVSRECERQNEERITSIVTPLITVIL
                     AYQKPDFPAWGEEARQLVLSPQYLEHVKSFVDSCDK"
     gene            10340..10933
                     /locus_tag="PLM83_01265"
     CDS             10340..10933
                     /locus_tag="PLM83_01265"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOK70074.1"
                     /translation="MVITDGRVILRDFVREDIEDRIRWETVETEWLLWDAPWELEEEE
                     PFSPDAYRDKMLRQLSTEKDPSRMRRSFQICINNGTRTHIGWCNSYNIDANYDYTDED
                     GYCTIGIDIPEQSARRQGYATAAWKLFIEYLLSNGVEDIYTQTWSGNERVLGLIRKMG
                     FEECARQVNYRMVRGQSYDGLTFKLNISSFRKLVYGK"
     gene            complement(11333..12316)
                     /gene="guaA"
                     /locus_tag="PLM83_01270"
                     /pseudo
     CDS             complement(11333..12316)
                     /gene="guaA"
                     /locus_tag="PLM83_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
     gene            complement(12338..12889)
                     /gene="hpt"
                     /locus_tag="PLM83_01275"
     CDS             complement(12338..12889)
                     /gene="hpt"
                     /locus_tag="PLM83_01275"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOK70075.1"
                     /translation="MHIIEDIQEVLISEEQIGSKVAELGAQISKEYEGKDLLLVCILK
                     GALVFMSDLMRCISIPVSIDTMAVTSYGASTVSSGVVKIIKDLDVPIEGRHVLIVEDI
                     VDTGLTLSYLVSTLKNRKPASVKVCTLLDKPDRRQASIVPDYNGFVIPDKFVVGYGLD
                     YMEKYRNLPFVGVPKDYVWNEEH"
     gene            complement(13004..13507)
                     /locus_tag="PLM83_01280"
     CDS             complement(13004..13507)
                     /locus_tag="PLM83_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK70076.1"
                     /translation="MLKDVEKDGIVKWEDVMASKGCPSSSRAEKGPVAVIECLQEIPC
                     NPCETGCPFGAISVGDRITNLPVLNEEKCRGCGNCVPVCPGLAIFIEDLSGSGDEAKV
                     TLPYEYLPLPERGAVVKATDRAGREICDATVVDVRKPAAFNKTAVITISVPKEFVHEA
                     RGIAFLR"
     gene            complement(13513..14253)
                     /locus_tag="PLM83_01285"
     CDS             complement(13513..14253)
                     /locus_tag="PLM83_01285"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOK70077.1"
                     /translation="MRIDVSLVPGEIAAKDVSGSVVVIIDVLRATSSIATALASGCEE
                     VIPAESIEEAITLANGYKRSDYVLGGERRGVPVEGFDLGNSPQEYDGPRVAGKKVIMA
                     TTNGTSAINKYGIAREIFTACFLNADAVIGACEAYDDCDLVLVCAGQDGRFCMEDTLC
                     AGLMATRIAHDKGAEMSDSAKAASLLYQQRKGSIEDELLRCDHGAYLVGIGYEQDVRY
                     CSRNSILDVVPKVYDRRVIRRARGGPEE"
     gene            complement(14267..>14667)
                     /locus_tag="PLM83_01290"
     CDS             complement(14267..>14667)
                     /locus_tag="PLM83_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOK70078.1"
                     /translation="EDKAGGYSIDLEPGVYHMDGEMALSFVRYRQDGLGDISRTARQQ
                     EFVKAFIREAGSFRAIPSYPNMLRVAQKCADTDMTVKEVAAVAWRLRRLDSDTINSAT
                     LPGYFKGAYWDPDTQGIEALVRSLQDSAQK"
BASE COUNT         3380 a         4414 c         3365 g         3508 t
ORIGIN      
        1 gcggttggag tcaaaactcc cgtccttccc acctgcacta ttatgtcgcg aactctagtt
       61 tgcacctgct gtgcggggta tttgtatgcc gaagcccacc tcggcgaatg cgaggtaaac
      121 ccgagctttc gttgaagatc tagcctgttg acttttacaa ccaccccgtc tatctcgtat
      181 ggaagcatgg gccgcaaatc ggccagtcga tggatgaact ccagagtctc ccccaagccg
      241 tcgcacaaga tgatctcggg attcacccta aaacccaatg agcgtataaa atcaagcgca
      301 tcccaatgcg tctcgatgcc gtaacgctct gcctccggca gatagtagaa gaatgcgtcg
      361 agcttgcggg acgcggtcac tttcggatcg agttgtcgca atgatccagc tgcagcattg
      421 cgtgggtttg cgaacatagg ttcacctctg ctcgcccttt cttggttgag ccgttcgaac
      481 tcggatttgc gcatgtagac ctctccacgc accgtaaccg aatcgggagc gcctttgggc
      541 aacaccaacg gcacagccct tatggtcctg agattatggg ttacgtcctc gccgcgttca
      601 ccatcacccc gagttgcccc aactgcgagc ctcccattct cgtatgtgag gctgatcgca
      661 aggccatcaa ttttcggctc ggctacgtac tctaccttgg ccaggccagt cgccctcttt
      721 atgcgcgcgt cgaacgcccg cagcgaaccc tcatcgaaca tgttgtccag ggacaacatg
      781 gggattgcat ggaccaccgg ctcaaagccg gccgacacag cgccgccaac cctctgtgtg
      841 ggggaatcag gatcagcaag ctccggatga gcctcttcta aggcaatcag ttcgcgcatg
      901 agcatgtcgt actcggcgtc ggtgatctcc gggtcatcga ggacgtagta tctccagtta
      961 tgatgacgta tcagttccct aagctcgtca actctgctgg ctgcgtcagt caactcagcc
     1021 acccccctcg gtcgctagat tctctccatg ggcgcaaagc tctgcgccaa atgcttgatt
     1081 ccccctccgg ggaacgccac tgttatgacc caatcattcc cgcctcttgc cacggccaca
     1141 accgttcctt caccgaactt ggcatgcctg acgcgatcac cggcagaata ctctgcacta
     1201 ccgtccccgc cgtggttttc gcctcccccg tcactgctgt cgccccggtt ccatgctgtc
     1261 ccagaccgcc cttccacgga gtgagcacgt ataaccggcg aagcgggccc gtccgatctg
     1321 ccccagaggc ttcgcctatc atgcatcgca tcggccagca gctcctttgg aatctcgccc
     1381 agaaatcgcg acggcgcgtt tgccatcgtc gaaccataga tcatgcgcat cacagaatgc
     1441 acaaggtata gcctgtcctt tgcgcgcgtg atccccacgt aacaaagacg ccgttcctcc
     1501 tcgagagagc gctcctcgcg ggcggatcgc gcaaggggaa agatattctc ttccatgcca
     1561 gcgatgaaaa ccacggggaa ctcgaggccc ttggctgcat gcatcgtcat cagtgtaatg
     1621 gaatcatctg aaaggtctgc cctgtccaga tcggtaagga gtgagacttc ctcgagaaaa
     1681 gctccaatat ctgtcggtcc accgccatcc tcaacagaat tctccatacg cctggcctca
     1741 aattcctgag tcacggtgag aagctctctg aggttctcta ttctgccctc ggcctccaga
     1801 gtctgttccc gttccagctc acgaatgtag ccagtctttt cgagaaccat ctctacaagc
     1861 tgagagaccg tagcctcccc tttgcgcgat tggaggccct ccatcaactg gagaaactgc
     1921 gataacccgc tcctggctcg gctcggagta tccgacgctc ggaccgccaa ttcgacccct
     1981 gcgtagagtg gaactccggc ctcttctgag agtctgcgga ctatggcaag tgtagcatca
     2041 cctattcccc tacgcggaac attgattata cgttcaaggg caagggaatc ctcggggttc
     2101 tcaatcactc gcaagtaggc caggacatcc tttatctcgc gccgctcgta gaacttgagg
     2161 ccgccgacga tcttgtacgg catgccccta ttcaagagga cttcttctat cgccctcgac
     2221 tgcgcattta ccctgtatag tactgcaaag gcgcgatacg cccttccttc aagagctgca
     2281 agccgctgaa tctcgtctgc aatgaacctt ccctcatccc tttcattctc ggcacagtac
     2341 acaaagatcg gagagcccga acctcgggaa gtccaaaggc tcttgtcctt acgccctttg
     2401 ttgttggcta tcacgcaatt cgctgcatta aggatgttct gggttgacct atagttctgc
     2461 tccagcttga tcacacgggc ttcgggataa tcacgctcga aatccaggat gttacggata
     2521 tcggcgccgc gccattcgta tatcgattgg tcgtcatccc ccactacaca caggttccta
     2581 ctccttgctg caaggagttt gacgagaatg tactgggcat ggttagtgtc ctgatattca
     2641 tctaccatca ggtattcgaa tctgtcctgc catttctcga gaatctcggg acattcccgg
     2701 agcaacctca ctgcaagggt tatgagatcg tcaaaatcca gagcattgcc tcgaacaagc
     2761 gtgctctgat aggccgcata tatctgcccc atcgtacgag ttctgggatc gcgtgcattg
     2821 gcctcaagtt cctcaggacc tatcaactca ttctttgcag agctgatggc agcgagcact
     2881 gcctgcggct tgaacatgtc gccggaaatg ttgaggtcat cgagacactt ctttaccacg
     2941 gccgtttgat cagattcatc cagtatcgta aagctcttct tgtaaccaag tgagggtatg
     3001 tcctctctca acattctcgc gcacaaagag tgaaatgttg atatccacat gaggtccgca
     3061 cgcgcaccaa cgagttcttc tactctgttc ttcatttcct tggcagcctt gttggtaaac
     3121 gtcacagcca gtattctgta tggaggcacc cttctttccg atatcaggta ggctgctcta
     3181 tgcgtgagga ccctggtttt tccgctccct gcgccggcaa gaatgagaag cgggccgtcg
     3241 acacaaacga cggcctctct ctgcggctca ttcaatgcgc caaggagatc tccgcccatg
     3301 cccctattcc tccaaaagcg ctatggcttt tctgatccgg gccaaagatc gatcccggcc
     3361 cagcagcaca aggctctcga agagaggcgg ggtgactcgg cggccagtaa cagcaatcct
     3421 cacaggcatc agcagctccc ctgctttcca gccaagctca gttgcggtcc ctctgagtgc
     3481 ctcctctatc ggctcatgct cccacgtcga taccttctcg agcgcgtcag cagacgcctt
     3541 aagcccgcgc gctatgtcgt cgtacgaaaa cttggctttg gtcttgagag cgctcgggtc
     3601 aagttccacg tcgtcgacga agaggaatcc aagcaaatcc ggcgcctcgg tgagcttgcc
     3661 tatcctgtcg gtgatgagcg ccactgcccg ttccagatag tcggtatcgt caaccgaata
     3721 gcctgcagcc tccataaaag ggcgaactct ggaggccaaa tcaggcacgg tgagactcct
     3781 gatgtagact ccgttgaacc agtccagttt cttcatgtcg aacacagcgt ccgattttga
     3841 cagcgtgtcc acggaaaatg cctccaccag ctcatctatg ctgaatatct cttgttcagt
     3901 gccggggttc cagcccagaa gggcaaggaa gtttatgacg gcctctggca gataacccat
     3961 ttccctgaat tcgccaatat acactgcgcc agccctcttc gagatcttgg tgcggtcagg
     4021 gttgagcatt agagtaaggt gtccgaactg aggtggctcc catcccatcg ctgtgtatac
     4081 gcatacttgg tcgaaagtgt tggtcaggtg ctcgactgcc ctgaatacgt gactcatctc
     4141 catgtaatgc tcgtccacaa cagtggcgaa gttgtacaca ggagtgccat cagacttgaa
     4201 tatgatgaaa tctccccgct ccgacatgtc tttctgttga tgcccgcgta taagatcgtc
     4261 aaaagacaaa gtgacatcgg ggacacgaag ccttaccaca ggccttcttc cttccgcctc
     4321 cagcctcgcg cggtccgcac tactcagttc cctgcacttt ccggaatatc gaggaggctt
     4381 tccggcagcc agcgctgcct tgcgctcttc ctcaagctct gaaggggtgc aatagcagta
     4441 ataggctttg ccctccgaaa gcaatttctc cacagtttcc ttgtaggtat ccatccgttc
     4501 cagctggcga tatggaccgt aaggccctcc tatgtccggc ccttcatccc acttgattcc
     4561 cagccatctc aagccttcaa ggacgccttc ttctgactct tttgtgcttc gttcaaggtc
     4621 agtgttgtca attctcagga cgaatgtgcc gccgtatttc cgcgcaaaaa gcatgttgta
     4681 cagagcagtc cgagccgtcc ccacatggca attgcctgtg ggcgatggcg cgatcctaac
     4741 ccttactttt ctatctttag acgatgttga gtccgcattc atggatcaaa agtcagctcc
     4801 tcttcgctac gcagtcgtgc ctatgaaatt tagttttgcc gtcttgatag caggcgcccg
     4861 gtatattccg cggccccccg caaccggcaa cagctctgtg tcgcgcgaca cagcaactat
     4921 gttcgcaaga attccgtcga gtattgaatc agtgaacctc agattccgca gcggccgaac
     4981 tattcggccg tcttcaataa ggtaagtgcc gtcccgcgtc atccccgtaa aaatcgtttt
     5041 cgtcggatgc accacattgg tgtagttgaa ccgcgttacg taaacgcccc gcccggtgct
     5101 ggccaccatt tccccaatgg acgattcccc ggggctcatc cgcagatgca gtgggtagcc
     5161 gcctttgagg gcgcccccaa caagggcatg cccggtcgac tctgtccccg ctttcttggc
     5221 tgcaagtctg tcaaagatca tgccggacgc cacgccgtgt tcgataaggg tcacgggccg
     5281 tcgcggaacc ccttccagat cgaatggccg cggcaagagg cccgggtcat gccagttatc
     5341 ctcgatagtc accatatcgg acgtgatctt tgaacctatc cgtccgctag cataacttct
     5401 tccctctatg aatgatatcc cattgatgcc catcaaagcc agggtctcca tcatgtcgac
     5461 tactgcagct ggctcaagaa tggcttcgta ttcgccaggc tctaatgcac caggattcct
     5521 ggaagaaagg cacttctcca cagccctttc tccgatagcc cgcgcgtcaa gattccccgc
     5581 tgaccaggac gccgcttcag cgtagcctga tccggcattg gaatcgtatc ccgtcatgat
     5641 caccgcgctt attgcggaat ctgtccctct catccacgcg ctggctcctt cagagtttgc
     5701 aatcgccatg ccagtaaccc tcgtacgaaa cgccccggag gcgttgagtt catgcttgtc
     5761 agctatggct gtgattgcct tcacagccat ggctctctcg cgggccgaaa aggatgccgt
     5821 tcgctcgtcg tagccaccag cttcctcata tctacacagc cctgccaaac ccggaaaatc
     5881 gggattttca ggttgaacca tggcgatctc caccgccagc gccgcagcgt ccctaacagc
     5941 ttccgaggtc agctgatttg tagtcgccca tccctcacgt tttccacaaa tggccctcac
     6001 atacaagatg tctgacgaga ccgaagcgtt ctgatggatc tcagaatttg caaacctggt
     6061 aaggttgtca acgtcgctca tgagtacagc ttcgacagca tcagctctgc aggctgaaat
     6121 ggccgcttcg actatctcct gcgcacgctc ctggcctacc atcttccgac acctaccttc
     6181 acattcctga acctcgcagg cgcagtgcca tgcccaacaa acatgacctg gctgggctct
     6241 ccctttccgc agttaggaaa gccccacagc acccattcgt cagaccccgc gataccatcg
     6301 catgaattcc aaaactctgg agttattcct gtgtatgttg gattcttcac catctccgca
     6361 agctggccat tgcgtatccg ccagccgatc tcgcttccaa attggaagtt taagcgcctg
     6421 tcatcaatgc tccaactctt tatcccgtcc atgaatatgc catcatcagt tccgcggatg
     6481 agggattcga gggtctcgtt acccggctca aggcatatat tggtcatcct tatgatgggc
     6541 attctatccc atccgctcgc tctcatacaa ccattgctgg tctttccaag ccttgcagca
     6601 gtctccctcg acatcagata cgcctcgaac atgccatttc taaccagatc ggttcggcaa
     6661 gaccgaaccc cttcatcatc gtacgcaaag gtaccaagcc ctcctggaat cgtcgaatcg
     6721 gcgtagatgt tgaccaagtc agatccatac ctgaagtctc ccagcttgcc aggatcaaga
     6781 aaactcgttc ccgcataaga cgcctcatag ccatagactc ggtccagctc cgtcggatga
     6841 ccgcaagact catgtatctg cagtgccaac tgattggtac ccagtatcag agtggtcttt
     6901 atctgcgggc attgttgggc cgcgaggaga gcctgagctt gtttcccgat ttcagccgcg
     6961 tgtccagcaa tgtccatctc ctctatccat tcgtaccctg cctgcctcac attgccttca
     7021 aacgtcccag gatacgatct gacctgcatc tctccgttgc caagagctat cgccttgatg
     7081 cccgccgaag tgaccgtggt ccgctgccga atccgcgccc cctcagagtt ggcataggcc
     7141 ttatcatccc ttatcgcatt catggacgcc gatgcatagc gcacatcgct tccgccggac
     7201 atcgcgcggc tgcaagcctc cctgagaagg tctatcttct gagccctcgg aactgaccaa
     7261 ggatctatgg cacatggcga ctcccactcc acaactacag gatcgagtgg cgcaagaaca
     7321 acgtcgcact tctttgccat tgcgcttgcc ctggcaatct ctactgcctc cgcagcaact
     7381 ctggcagcct cgctctcgct cacaacacag cttgcggcga atccccatgc cccatccaca
     7441 cgtgccctaa cgcctatgcc atgcgagact ccgctcgtgg cccgctccac aacaccattg
     7501 cgaacagaga gcctctcctc cgtctgatgc aacgcacgaa catccgcata ttccgctccg
     7561 gtcgcgcagg ctctgtctag cgctatttcg agaactctca agccatcaca cctccctgga
     7621 tcgtttgata tcaccaacct gacacagcag aatgccggct agtatgagaa tggccccaat
     7681 cgcctgctgc acgctgagac gctcccccaa ccatagccgc gcaaagatgg atgtaaaaat
     7741 cggttcggtc tggagtatca gggccgcccg tgtaggcggc gtatattttt gagctgtagt
     7801 ctgaacgaga taagcaagcg ctgttcctgt tgcaccaagg taggctacgg agaggactaa
     7861 gtccttgtcg aattggaata tctggactat gtcggatcct gcagccaaca ccgaaaagac
     7921 cgcagcccat gccatctggg taaatgacag gagcaagccg tccatggtcg gcgaatacct
     7981 ggctaggagg attatgtgga ccgcaaagca aaatgcgcac actaagatca gaatatcgcc
     8041 atatgtcgga ggcgacagcc tgtccagtgt gagaaggcca agccctgcaa ctgcaacacc
     8101 aacgcctgtg atctgatatg gcatgatgcg attcttgagg accttctgtt caaggaaaag
     8161 cgtaaaaact acgtgcatcc ctgtgatgaa cccggccttg gacgcagtag tcccctgcaa
     8221 cccgcgagtt tgggttaaaa acccgagcat gagggggatt gccagcgcga accctgcctt
     8281 acagtattcc cagtccagtc cacgaaggcg ccgacccgca acgagcccga gaagaaccgt
     8341 agcaagtaca aatcttgccg caataaaagt ggccggattc gtgtcgccca gcactgtctt
     8401 gactatggcg aagctcgttc cccatataac gcatatccct actaggctca ggtcggccag
     8461 gattgatttg tacgacccca agcgccatga tttcaactgc tgtccctact cccactaagc
     8521 gattacagac acctaaacgt tccgattatg tcgcacaaaa gcaccattat atatccgaac
     8581 agcacctccc cactcccaga acttcgcaga agccaaatac cccttgagtt gctataccta
     8641 caactgatat agccaatcta ctgccggtgc cagtcgcccc aatcctcttt caggaagtca
     8701 gtgcaacccg catcccaatg atacccattg cagcttgcat cggaataggc attgtattac
     8761 atggctttgg gatctagcaa catttgctgc ctaagtcata gttcttcaag ctccggcaca
     8821 tgcagctcaa ttctctgccg ttacttgtca caggaatcca caaacgattt cacgtgttcc
     8881 aaatactgcg ggctcaatac cagctgccta gcttcttctc cccaggcagg gaaatcaggc
     8941 ttttggtatg ctaaaatgac cgtgatcaac ggcgttacaa tcgatgtgat cctctcttcg
     9001 ttctgacgct cgcattctct tgatactccg tattttccat ggtaaagcat atcatactga
     9061 tcttcaaaag ccgttcccgc ttcgactgac aaactagact tcacattcga aatatcgttt
     9121 ccccgatagc cttgacctag ataattgtaa tcaaacatca tggcggcagt gtgatctttt
     9181 cttacgagca tgttcgtcca ataaaagtcg ttgtagttca aagttcgcac caaccccgcc
     9241 agttcctttt ggaacaggcc tacatggttt tgcagataac aggtaatatc cttaagagaa
     9301 ggatcccttt ccgacagacg attgaggttt gctatcgtca ggcaatctgt ctcacagtac
     9361 agttcaggca gctcaggaat gttgatcccc ttctcgtgga aatgaaaata ccactcggct
     9421 aacctgccgg caatttgctc atctctgaga tcgtccggga tgcctaaacg ccacgtcttg
     9481 ctctcagcga tgtcttccaa aacaagacat cgctgtgcat actcgagaac gtccatcgtc
     9541 ggaatgccca agcgatgaag aagctgataa ttatggatct ccctcaggtc gtcatctttt
     9601 tcaaagtact tgattacagt ggggcttcca tcgaaggaac aacgaaaaac ccacacccga
     9661 tccttgctgc gaatcagctc aaaccgatca atctcgtgtg agtggagttg gcctaagata
     9721 tggcgcctaa cccgttcttc agcatcctgc cttggttctc cagcgatcgc tcgacccatc
     9781 cttttgcttc tcctcatcta ggcgatcgtc cctcaagatc cgcgccactg taaatctcct
     9841 tagcttccat cgcctagaat gccaaatgct ccttcatgca aattcaatct ctatgctaat
     9901 aacgtcatta taccatgaat ccctgagatc cgaccccggg cccaagcagt tagcccctgc
     9961 acatctcggc tgcgcaacca cgcactctgc gattgccaaa cacacccgtt ttcggacgtg
    10021 cagttcgatg cgccctgtga tactatccat gttgctggcc cctttcgcgt gtagctctgt
    10081 gtcaaggtag gctcttcccc tgggtatatc ctaccgatcg cgatagatac ctcattcacg
    10141 gccccagaaa gtgagccgga aggttgtcac aatagcgtcc cactaatccg ttttgaacag
    10201 ctttctggca tcgttcaaac ggcagaaatc gacgcaaaat gcactgaccc agagcgtacg
    10261 cgccagaaat gggcctcagt ctagtccttc aagaatctcc tcccatttgt catgaagatt
    10321 ccctattgag gaggattgta tggtaatcac agacggcaga gtgatcctga gagattttgt
    10381 gcgagaggac attgaagaca ggattcgctg ggagacagta gagacagaat ggttactatg
    10441 ggacgctcca tgggagcttg aagaggaaga accgttttcg cctgacgcat atcgagataa
    10501 gatgctgcgc caactgtcca cagaaaagga tcctagtaga atgcgccgga gttttcagat
    10561 atgcatcaac aatggaactc gaacgcacat tggttggtgt aattcataca acatagacgc
    10621 gaattatgac tacacagatg aggatggata ctgcacgata ggaatcgaca ttcccgagca
    10681 aagtgcacga cgccagggtt atgcaaccgc tgcatggaag ctgttcatcg aatacctgct
    10741 gtccaacgga gttgaagaca tttacacaca gacctggtcc gggaatgagc gcgtcctggg
    10801 cctgataaga aagatgggat ttgaagagtg cgctcgacaa gtcaattacc ggatggtgcg
    10861 tggccaatca tatgatggac tgacgttcaa gctgaacatt tcgagttttc gaaagctcgt
    10921 atatggcaaa tagcgcccac ccgtggctga gctaagccct tcatggattt tctctgatgc
    10981 cgttacttgc atgcgcagca tcagctcgcg gcgtgtgttt ttgtgaaacc tggattcagc
    11041 gcacctactg tttagccggt catataacaa gccatatgga atacgccaca tgtcagatgt
    11101 gaaattcagg tttacctgtc ctcctgattg caattccagc cctaggcaaa atacgacgcc
    11161 ggcctcctat gcttatccca tgggaagccg acgttctgtc tatattcatt ccctagctct
    11221 gaaattatcc gccttttgga ttacatcgaa gcaaatggag gcgaactaga agtagagccg
    11281 tcctaaaaag cctgaaagag tctgttttgc ctgataggtg caaatctgca cctcattccc
    11341 actcaatagt ggccggcggc tttggagtaa tatcgtagac cactcggtta actccgggca
    11401 cttcatcaac gatgcgtcgg ctgatctcct ccaatgcagc atacggaagc ctcgcccaat
    11461 cagcgctcat gccgtctggg ctcttcaccg ccctgattgc tattgggtag gaataggtgc
    11521 ggtcgctgtc gctcactccg gtgctgcgaa tcgcgggaag aaccgcgaaa tactgccaga
    11581 gctcgcttgc ctcaggaagc ttcccgattt cttcacgcac tatggcatcg gcctctctta
    11641 aaacctccag ccgttctcgg gtgacctctc caattatcct aacagccagc cccggcccgg
    11701 ggaatggctg cctcttcact atggtatcag gaagccccag ttccttgccg atgaggcgga
    11761 catcatcctt gtagagcatc ctcagaggtt ctatcagttc gaacctgaga tcctccggaa
    11821 gtccgcccac attatgatgg gacttgacct tgactccagg tttctggcta gagacactct
    11881 caaccacgtc aggaaggaga gtcccctgga ccaaatatgg aacatttccc agcttttccg
    11941 attcctcttc gaagacccgt atgaactctg cgccaataat cttccttttt gcctccgggt
    12001 ccacaacgcc ttcaagcttc ttcagaaacc gctcttttgc atccacaact atgaggttca
    12061 tgtggtaatg gccgcggaaa acgttctcca ccccttcagc ctcgcccttc ctaagcagac
    12121 cgttgtccac aaagatgcag gtaagattgg cacctatagc tttatggaca agcaccgcag
    12181 caacagatga gtcgacgccg cccgacaatc cgcaaactgc tcgctccgct cctatctgct
    12241 gcgaaattgc tgaaatcgcc tcatctatga atgagcgcgg gtcgcggcca tcctgtagac
    12301 cgcctgcatt gtgtagatgt ctctctttca tgaaatttca atgctcctcg ttccacacat
    12361 aatctttagg cacgcccaca aaaggcaggt ttcggtattt ttccatgtaa tccaagccat
    12421 aacctacaac aaacttatcg ggaattacga atccattata gtccggaaca atagacgcct
    12481 gcctccgatc cggcttatcc agaagtgtgc ataccttcac agatgctggc ttgcggttct
    12541 tcagagtcga aaccaagtaa cttagggtaa gccctgtatc gactatatcc tcaacgatga
    12601 ggacgtgtcg gccttcgata ggaacatcaa gatcctttat tatcttaacc actcctgagg
    12661 agacagtcga tgccccatag ctagtaacgg ccatggtatc aatgctgacc ggaatcgata
    12721 tgcatcgcat cagatccgac atgaatacaa gggccccttt gagtatgcac accaacagta
    12781 aatcctttcc ctcgtattcc ttggatattt gtgcccccaa ttccgccact ttggagccta
    12841 tctgttcctc tgagatcaaa acttcttgaa tgtcttcgat gatatgcatt gaaggatgca
    12901 cagacactag ccgattcctc ccatcacgtc tgcaggccgg cccgaagacc tcatacggac
    12961 cggcctgctc attaacatat tgaaacaggc aaatcacagc acactaccgg aggaaagcta
    13021 tccccctggc ctcatgcaca aattccttcg gcacagagat cgtgatgaca gcagtcttgt
    13081 tgaaagccgc aggtttcctg acatctacga ctgtcgcgtc gcatatttcc cgtccagctc
    13141 tgtcagttgc ctttacgacg gcgccgcgct caggaagagg caagtattca taggggagcg
    13201 tcaccttggc ctcatcgcca gatcccgata gatcctcgat aaagatggca agcccaggac
    13261 agacaggaac gcagttccca catcccctgc acttctcctc attcagcacg ggaaggttcg
    13321 ttattcgatc cccaacggaa atagcgccga atgggcatcc cgtttcacac gggttgcaag
    13381 ggatctcctg aaggcattcg atgaccgcca ctgggccttt ctcagccctt gaactgcttg
    13441 gacatccctt cgaagccatg acgtcttccc acttaacaat accgtccttc tcgacgtcct
    13501 tcagcaaacc actcactcct ctggcccacc acgagccctt cttatgactc tcctgtcata
    13561 gacctttggc acaacgtcaa ggatggaatt tcgtgagcaa tatctcacat cctgctcgta
    13621 accgattcca accagataag ctccgtggtc acagcggagc aactcatcct caatcgaccc
    13681 ttttcgctgc tggtacaaga gtgaagccgc cttggcagag tcggacatct ccgctccctt
    13741 gtcatgtgca atcctcgtag ccataagtcc tgcgcaaagc gtgtcttcca tgcagaacct
    13801 cccgtcttgc ccggcacaga ccagcaccaa gtcgcagtcg tcataggctt cgcatgctcc
    13861 tataacagca tcagcgttaa gaaaacacgc tgtaaaaatc tctcgcgcaa ttccatactt
    13921 attgatggcg ctagtgccat ttgtagtcgc catgatgacc ttcttcccag ccactcgggg
    13981 gccatcatat tcttgcggag aattgcccag atcgaatcct tcaacaggaa ctccccgccg
    14041 ctcccctcct agaacataat ctgacctctt gtaaccattg gccagggtta tggcctcctc
    14101 tatactctca gcgggaataa cctcctcgca accagaagcc aacgccgtag caatgctgct
    14161 ggtagccctg aggacatcaa ttattaccac tacagaccca gatacatcct tggctgcaat
    14221 ctcgcctggg accagtgaaa catctatccg cattgcggtt ctcctttcat ttctgtgcag
    14281 aatcttgaag tgatcgaacg agcgcctcaa tgccttgagt atccggatcc caataagcac
    14341 ccttaaaata acctggcagc gtagcagaat ttatggtgtc agaatcaagc cttctcaaac
    14401 gccaagccac ggcagccact tccttgacag tcatatcagt gtcggcgcac ttttgggcta
    14461 cgcgcagcat gttcggataa gatggaatag cccgaaagct cccagcctcc ctaataaacg
    14521 ccttcacgaa ttcttgctga cgcgcagttc gggatatgtc ccccaaccca tcttgccggt
    14581 atctgacgaa actgagagcc atttcaccat ccatgtggta gactccaggc tccaaatcta
    14641 tggaataccc gccagccttg tcctcat
//
LOCUS       DAOSAI010000015        14332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_2875_length_14332_cov_3.648245, whole genome shotgun sequence.
ACCESSION   DAOSAI010000015 DAOSAI010000000
VERSION     DAOSAI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14332
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2875_length_14332_cov_3.648245"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLM83_01295"
     CDS             complement(<1..392)
                     /locus_tag="PLM83_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOK70079.1"
                     /translation="MREALYYERENDKVRCLLCPHRCLITEGKGGICRQRKNHGGKLI
                     AEGYGRVSSIAMDPIEKKPLYHFYPSSSILSAGGLHCNLRCSFCQNWHIAHREKPVRF
                     IPPEDLVEIALSCGGIGIAFTYNEPVIW"
     gene            complement(418..1821)
                     /gene="amrA"
                     /locus_tag="PLM83_01300"
     CDS             complement(418..1821)
                     /gene="amrA"
                     /locus_tag="PLM83_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOK70080.1"
                     /translation="MGELVLAALAPHPPIIVPGVGGREAAKAQKTIDAMEELAREFAA
                     ARPDTVVVISPHAPVFADAIAIDGRPHLSGDLAQFGADVELDFYSNLELVQAIEDEAH
                     AAGISVAVLEPALARRYRVPSGLDHGAVVPLYFLAKEHYPFRLVAVSMGVGALNELYT
                     FGMCMASAIDKSQYRVAVIASGDLSHRLSPDAPAGYAPEGKIFDEAVVGALGTMDVAA
                     LARLDDALIEAAGMCGLNPIIMMLGSLDGIEVESEVLSYEGPFGVGYAVVALRPKGRS
                     EEARRFHELVEGGKEKTRKLRHSESAFAALARRAVEAYVCRGVVVEPPDPLPEGMQGR
                     AGVFCTLHKRGQLRGCIGTIAPAQKNIAEEIIHNAISAGTQDPRFDPVEEEELEDLVY
                     SVDVLMPPQPVKSVSELDAKKYGVIVRSGYKSGLLLPDLEGVDTPEKQIEIALKKAGI
                     RQNESYSIERFEVERHN"
     gene            complement(1809..3107)
                     /gene="murA"
                     /locus_tag="PLM83_01305"
     CDS             complement(1809..3107)
                     /gene="murA"
                     /locus_tag="PLM83_01305"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOK70081.1"
                     /translation="MELVIAGGKRLVGSVRAGGSKNAAIAIMAGSLLAEGDTIIENAP
                     GARDVSAMSAVLREMGVSIRRAGPSALRVRTHRLIHHEPPYELAKTLRASSLLLGPLL
                     ARKGKASIPLPGGDSIGSRPMDLHIKGLRALGADIEVEHGYIVAKAPRLEGARVYLDF
                     PSVGATENIMMAACLANGTSIIENAAKEPEIVDLANFLTLMGANIRGAGTDTIRVDGV
                     DSLRGVAYSVIPDRIEAATLLAAGIITHGQVKVGSIIPTHLESVLSKFSEMGASITVG
                     DDYVEAAMDGPIQPCDTKTLPYPGFPTDMQPQLSAVLSVASGVSVITESVFESRFFHV
                     DELKRMGADIRLEGRSIIVEGVPGLTGARVHAGDLRTGAALILAGLAAEGETVISGAE
                     NIDRGHDDLCSKMNSLGARVKRQDSQRCVAAGEENETWVN"
     gene            complement(3207..4463)
                     /locus_tag="PLM83_01310"
     CDS             complement(3207..4463)
                     /locus_tag="PLM83_01310"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOK70082.1"
                     /translation="MKRPVIFIALPVRTAVLIGIAVLIGVVIYAVQVLVVTTLAPVRD
                     VVSGKTVAIDAGHGGPDSGARGKSGIIEKDINLHIALELRQLLGRVAVYTVMTRESDH
                     GLIADGEDITGSRKRMELERRANLVNANSPDIFVTIHTNSFPEPQWSGAQVFYNAASA
                     EGRKLAVHIQTELVNRLGPNTRKARPADIYLLKKVKAPSALVEVGFLSNPREERLLAD
                     AAYRVKVAEAVFHGIMAYFMDSQGEAGNVLAPDDDSDEAWGLVTKVGTATSQLTPDST
                     ILYFGGPTNLDDSLMPEAREIPGLKSLNKIEALAAVVQATISGPGKDSILQPTIPRGT
                     VLRSVRLQGDTAVADFSAELVMNHWGGSRSEEMTVYSIVNALTEFSYVKKVEILVEGK
                     PLQTISGHMQIEGPLERNLSIVHFRT"
     gene            complement(4634..5245)
                     /locus_tag="PLM83_01315"
     CDS             complement(4634..5245)
                     /locus_tag="PLM83_01315"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOK70083.1"
                     /translation="MCALSEHYFTTSPTSPHERRIISYTPRGGRELSFETDAGVFSKT
                     RVDRGTDQLIQAIRISPGQSLLDLGCGYGVVGISIASLHPECEIWMTDINERACELAR
                     ANARRNGVERVTVVSGDGFDPVRGRMFHVIATNPPIRAGRAVLYRLIEEAHDHLEPGG
                     SFYAVVRTKQGAASLKAFIAQTYGNADEPEIGGGYRVIRAFRE"
     gene            complement(5349..6761)
                     /gene="ypeB"
                     /locus_tag="PLM83_01320"
     CDS             complement(5349..6761)
                     /gene="ypeB"
                     /locus_tag="PLM83_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HOK70084.1"
                     /translation="MTDGTSGARGKMRNWPQALLLVIVIVSLVFAYSQWAAKQRLEVA
                     VGNQYFRSFFDLLGNVDNVRVALSKSLASGSPRLRVGALSDVWREASNAQANLNNLPV
                     THKVLARTSAFLTQVGDFAFTTARKVANQQVMSEDDWNKLVDMKKQSEDLARGLADMQ
                     AQVSTGKVNWVRVAEQAKSEVKPKPGTSATPDVVKDGFTRVDEQVQTFPTLIYDGPFS
                     DHIEQIRPRGITGAEITADQAKEVARRFVPFDASSHSATVEENIKAKGDIDIYRVRLD
                     APKRSGKPDAVVDVTKKGGHVVLMNVARDLDKKTIDLAESVKRASAFLSKVGMVNMTP
                     TFASEVGDVSIVPFVYTQDGVVIYPDQVKVKVALDNGDILSYDALAYLTSHTMRRLPK
                     PSIDADEARELLSPALTVTESARLAVIPVETVDTPEAFCWEFRGTSYGEQFFVYINAE
                     KGTEEKVLQLITTSEGSLTM"
     gene            complement(6778..7227)
                     /locus_tag="PLM83_01325"
     CDS             complement(6778..7227)
                     /locus_tag="PLM83_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOK70085.1"
                     /translation="MRMPNARILVFVVLAVAVVIGTVAHIGRAQSRSGDVNLLARLIA
                     AEAEGEPYQGMVAVGAVMLNRVASPKFPNSISGCVFQPGAFESVSNGLIWRRSPSRQA
                     ISAARDAMNGYDPTYGCVFFWNPAKPVSGWIWSRSIAVKIGKHVFAR"
     gene            complement(7266..7976)
                     /gene="sleB"
                     /locus_tag="PLM83_01330"
     CDS             complement(7266..7976)
                     /gene="sleB"
                     /locus_tag="PLM83_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HOK70086.1"
                     /translation="MNLKTPIMGLTVGAMIFIVVLLCAATPAKAETRPTLYWGSRGRS
                     VRLVQWKLSDWGYYRGWVDGVFGANTHRAVLAFQRKNGLKVDGLVGPQTWRALGFGYT
                     VAAEAPAAYKPAPMASRSASVDLVARAIAAEARGEPYRGQVGVGAVLMNRLKNPKFPH
                     SLSAVIFQPNALESVSNGLYWRRTPDQIAYSAARDAINGFDPTYGSLFFWNPAKPVSG
                     WIWTRNIIVKIGKHVFAR"
     gene            8282..9280
                     /locus_tag="PLM83_01335"
     CDS             8282..9280
                     /locus_tag="PLM83_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOK70087.1"
                     /translation="MMHTIGVVFNLKKDFPGLVGKANSVPDLAAECEEEETVDMLSSS
                     LAKIGEKVMLFPHKSDLLERLDEARPDIVFNIAEGWAGRNRESLIPALLELLEIPYTG
                     SDALTLGIALDKAMAKRVASTVGVRTPRYIKVSSIEDLSSMNMDFPVFVKPNYEGSSK
                     GIRNSSRVRNMDELTEKVRWILETYSQPAIVEEFLPGREFTVGIIGNGRPHVLPIMEV
                     CPGNRDSADCEFVYSYETKSGNLERFRVPAPIPEHLASEIADMAVSVFEVMECRDVAR
                     IDFRLDAEERPHFLEINPLPGLSKVSLLPLQARAAGMTYDDLVKWILTSAWSRYWH"
     gene            9471..9722
                     /locus_tag="PLM83_01340"
     CDS             9471..9722
                     /locus_tag="PLM83_01340"
                     /inference="COORDINATES: protein motif:HMM:NF019199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOK70088.1"
                     /translation="MDNRVRCSVNNCHYWNEGNYCHASQIMVTSDSIANQLPDSYDAL
                     QASIAEPRPAAHIGETCCKTFAPKGTSDTTVRSDRTTRM"
     gene            11206..12957
                     /locus_tag="PLM83_01345"
     CDS             11206..12957
                     /locus_tag="PLM83_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70089.1"
                     /translation="MKRTEHLKDFFAKYWWRYVLGILTLIIVDALQLVVPKILGNVTD
                     ALREGSLTPEGLKKHVLIILAIACGIAIGRFLWRIFIMGTARLLDFELRNKLFAHLQK
                     LPARYYTEHKTGELMAHATNDIPSVRQAFGQGFILITDSIFMTTATLIILFRTVPIRL
                     AIFALLPFPLLAVSTAAFSKIINPRHLAVHEAFGELSDRAQESIAGIRVTKSFVQEDA
                     EIAKFMAVAQKNVDANIDLVKIWGLMSPLSGLATALAYAVVLRYGSSMIMYQQISLGD
                     FVAFTSYLAMLVWPMIALGWVINVMERGFTALDRLNAVLNEVPEVADEPGAIELEDVT
                     GTIEFRNLTFSYRDGVPPALSDISFKIDAGKTLAVVGRTGSGKTTIVNLLTRLYNPPE
                     GSVFIDGHDIRNVTLSSLRNQIGCVPQDTFLFSCTIGENIAFADRDFSPEEICHYAKI
                     AHVYDDIMGFPEGFDTKVGERGVTLSGGQKQRISIARALITEPKILILDDSLSAVDTQ
                     TEESILRGLSEFMHDRTSIIISHRISTVKDADEIIVLDDGKIIERGTHASLLAQAGLY
                     SEIYEKQLLEEELASRS"
     gene            12980..>14332
                     /locus_tag="PLM83_01350"
     CDS             12980..>14332
                     /locus_tag="PLM83_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70090.1"
                     /translation="MNGYEEEEQIIGKVYDSQLIRRLMSYAAPYRKTFVLGIIVIMII
                     AGTDLVRPYLIKTAMDDYISGYDAPMARYEPEAVPTGIRDNRMIHYNGHVYVRIKDLP
                     SNHADEYEPEALFQLLNISDQYVLISGALPPDHGPAQLATTDSGLVLIVDEVEHPAQI
                     IEGHENIGIFREQDLAAVSRLAIILLLVGALAFVLNYGQTVLLQRAGQRIIYEIREEV
                     FTHLQHMDVAFFDTNPVGRLVTRVTNDTETLNEMYTSVIVNLFKDVFMLVGIIVVMFR
                     LDTQLALLSLAALPLIIVSTIIFRTRARQAYRQVRTALARINSTLAENISGMKIVQIF
                     NRQQRNYDDFVNVNREYYRAGIGEMMVYAIFRPIMELIGTGALALVVWYGGRNVISGT
                     MQFGMVYAFINYVSMFFGPINDLTEKYNLMQAAMAASERLFQLMDTEIMVKDRENPVS
                     V"
BASE COUNT         3396 a         4028 c         3576 g         3332 t
ORIGIN      
        1 caccatatga cgggctcgtt ataggtaaag gcgatgccga tgccgccgca agagagcgcg
       61 atttctacca gatcttcggg aggaataaac cgaacgggct tctctcggtg ggcgatgtgc
      121 cagttctggc aaaaggagca gcggaggttg caatgaagcc cgcctgccga gagaatgctg
      181 cttgaagggt aaaaatggta taaaggcttt ttctcgatcg ggtccatcgc tatcgaggaa
      241 acccttccat atccttcggc aatcagcttc ccgccatggt ttttcctctg cctgcagatt
      301 ccgccctttc cctcggtgat gaggcagcgg tgagggcaga ggaggcacct taccttgtcg
      361 ttttccctct cgtaatacag agcttccctc atgttccaaa acctttaatt tgcgacttta
      421 attgtgccgc tccacttcaa agcgctctat agaatagctc tcgttttgcc ggatgccagc
      481 ctttttgagc gctatctcta tctgcttttc gggagtgtcg acgccctcga ggtcggggag
      541 aaggaggcct gacttatagc cgctccttac tatcaccccg tatttttttg cgtcgagctc
      601 gctcacgctc tttacgggct gtggcggcat gagcacatct accgagtaaa ccaggtcctc
      661 aagctcttcc tcttcaacgg ggtcgaaacg cgggtcctgc gtgcctgcgc ttatggcgtt
      721 atgaatgatt tcctcagcga tgtttttttg cgcaggggcg atggtgccta tacagccgcg
      781 cagctggccc cttttatgga gcgtgcagaa cacgcctgcc ctcccctgca tgccttcagg
      841 gagcggatca ggaggttcca caactacgcc tctgcaaaca taggcctcca cagctctcct
      901 agccaatgct gcaaaagctg actccgagtg cctcagcttc ctcgttttct cttttccgcc
      961 ttcaacaagc tcatggaaac ggcgggcttc ctctgatcta ccctttgggc gcagggcaac
     1021 gacagcatac cctaccccaa aaggaccctc ataggagagc acttccgatt ccacttcaat
     1081 accgtccagc gatcctagca tcatgataat cggattgagg ccgcacatgc cggcagcctc
     1141 gattagtgca tcatcgagcc gagctagggc tgcaacgtcc atggtcccaa gggcgccgac
     1201 taccgcctcg tcgaagatct tgccttcagg ggcgtatcct gccggtgcat ccgggcttag
     1261 ccggtgcgac aggtcaccgc tggcaatcac ggcaacgcgg tattgtgact tgtctatggc
     1321 agaagccatg cacatgccga acgtgtaaag ctcattcaat gcgcccacac ccatcgatac
     1381 tgcaaccaat ctgaacggat aatgttcctt agcgagaaaa tacagtggga caaccgcgcc
     1441 atggtcgagc cctgacggca ccctgtaacg cctagcaagc gccggctcaa gtacggccac
     1501 agaaatacct gcggcgtgcg cttcatcttc gatggcttgc acaagctcta gattgctgta
     1561 aaagtccaac tcaacatcgg cgccaaattg ggccagatcg cctgaaagat gcggacgacc
     1621 atctatggct atagcgtctg caaataccgg agcatgaggc gagatcacca ccaccgtgtc
     1681 cggcctcgct gcagcgaact ccctggccaa ctcctccatt gcatcgatgg tcttctgagc
     1741 ctttgcagcc tcacgccctc ccactcccgg aacgattatt gggggatgtg gagcaagagc
     1801 tgccaggact agttcaccca tgtctcattc tcctcccctg ccgcgacaca cctctgagaa
     1861 tcctgccgct tcacacgggc gcccagagaa ttcatcttcg agcagaggtc atcgtgtccg
     1921 cggtcgatat tttccgcccc agagatcact gtttcgccct ccgcagccaa ccctgccaag
     1981 atcagcgccg cacctgttct tagatcgcca gcatgtaccc tagcgccagt gaggccaggt
     2041 accccctcga ctatgatgct cctgccctca aggcgaatgt cagcccccat cctcttgagc
     2101 tcgtccacgt ggaagaaacg gctttcaaac accgactcag taatgacgct cacccctgat
     2161 gcgacggaca ggacagccga aagctgtggc tgcatgtcag tgggaaatcc tggataagga
     2221 agggtttttg tgtcgcatgg ctgtatcggg ccatccattg ctgcctcaac atagtcatcg
     2281 ccaactgtta tggaagcgcc catttcgcta aacttggaaa gcacagactc aagatgcgta
     2341 gggatgatac tgccgacttt aacctgccca tgggtgatta ttcccgctgc cagaagagtg
     2401 gcggcctcaa tcctgtcggg aattacggag taagcaactc ctctcaatga atcgaccccg
     2461 tcgacgcgaa tcgtatcagt cccagcccca cgtatgtttg cgcccatcaa ggtgaggaaa
     2521 ttggcaagat ctacaatttc aggctcttta gcggcgttct ctatgatgct tgtcccattt
     2581 gcaaggcaag cagccatcat aatgttctct gtggcgccaa cgctgggaaa atcaagataa
     2641 actctggctc cttccaggcg aggagccttg gccacgatgt atccgtgttc aacctcgatg
     2701 tcagcgccaa gggcgcggag ccccttaatg tgcaagtcca taggcctcga tccaatactg
     2761 tcgccgcctg gcaatggaat gcttgccttc ccctttcttg ccaggagagg gcctagcaaa
     2821 aggctggaag ccctgagcgt cttggcgagc tcatatggtg gttcatgatg aatcaagcga
     2881 tgtgtgcgaa cacgcagagc tgaggggccc gccctcctaa tactgacccc catctctcga
     2941 agcactgccg acatcgcgga gacatccctg gcgccaggcg cgttctcgat aatggtatcg
     3001 ccttcagcca agagtgatcc tgccattatc gcgattgcag cgttcttgct tccaccagcg
     3061 cgaaccgacc ccacaagccg cttgccacct gctataacta gttccacagt tccgacctcc
     3121 agggcacctt gcatttccaa tgcgcgccta tgaatagcta ttcttcatcc ggcgcctttt
     3181 atccttgtgc tccaacttca ccttgactac gttctaaaat ggacaattga caggtttcgt
     3241 tccagagggc cctctatctg catatggccc gagatagtct ggagaggctt cccctctacc
     3301 agaatctcaa ccttctttac atacgaaaac tcggtcaggg cattgacaat cgagtacacc
     3361 gtcatctcct ctgaacgact gccgccccag tgattcatta ccagctcagc gctgaagtcg
     3421 gccacagctg tgtctccctg cagtctaact gaacgcaata ccgtgccccg cggaattgtt
     3481 ggctggagta tgctgtcctt gccaggccct gatattgtcg cttgcacaac tgcggcgagg
     3541 gcttcaatct tgttgaggct tttcagtccc ggtatctccc tggcctcagg catcagcgaa
     3601 tcatcgagat tcgtgggacc accaaagtag aggatggtgg aatcaggagt aagctgagat
     3661 gtagcagtgc ctactttcgt aacaaggccc catgcttcat ccgaatcatc gtctggcgca
     3721 agaacattgc ctgcttcacc ctggctgtcc atgaaatagg ccataattcc atggaatact
     3781 gcttctgcaa ctttcacgcg ataggcggca tctgcaagaa gcctttcctc gcgcgggttc
     3841 gacagaaacc ctacctctac aagggcagaa ggcgccttta ccttcttgag caagtatatg
     3901 tcggctgggc gagccttccg agtgttgggt ccgaggcggt ttaccagctc agtctggata
     3961 tgtacagcca gtttcctgcc ctccgctgac gcggcattat agaagacctg tgcgccggac
     4021 cactgcggct caggaaagct gttggtgtgt atcgtgacga atatgtctgg agagttggcg
     4081 ttcacgagat tcgcccgccg ttcaagctcc atgcgctttc tggatcctgt gatgtcctct
     4141 ccgtctgcga tcaggccatg gtctgattcc cgggtcatca cagtatacac agctacgcgc
     4201 cccagcaatt gccttagctc caatgcgatg tgaagattga tgtccttctc tatgattcca
     4261 ctttttccgc gggcgccaga atccgggccg ccatgtccgg cgtctatagc cactgtcttt
     4321 ccggagacga catcgcggac cggcgccaat gtagtgacaa caaggacctg cacagcatag
     4381 atcacaacac caatcagcac tgctatacca atcaagacag ctgtgcgcac cggcaacgcg
     4441 ataaagatca caggtctttt catttcgcag gcctccaggc ggcaaggctc gcgacttatg
     4501 cggcctcagc tcctaaacag aatatgccgg aggcaccccc caaatgacct gtgtccgata
     4561 tgcctaagct ccatgctgcc cacagctttt gtccggcctg acgacctgct ccaaaatgcg
     4621 caagactgac tggtcactcc cggaaagccc ttatcactct atagcccccg cctatttcag
     4681 gctcatcagc gttgccgtag gtttgagcaa taaatgcttt gagagaagca gccccctgtt
     4741 tggtgcgaac tactgcgtag aagcttccac caggctcaag gtgatcatgg gcctcttcaa
     4801 tcaaacgata caggaccgcc cggccggcgc ggataggcgg attggtcgct ataacatgga
     4861 acatcctgcc ccgaacaggg tcaaaaccat ctcctgacac aaccgtcacg cgctccactc
     4921 catttctgcg ggcgttggcc cgggcaagct cgcaagcgcg ttcattgatg tcagtcatcc
     4981 atatctcaca ttccggatga agtgaggcga tggagatccc aacaactccg taaccgcatc
     5041 ctagatccaa gagggactga cccggactaa tccggatagc ctgtataagc tggtctgtgc
     5101 ctcgatccac acgagtcttc gagaaaaccc cggcatccgt ttcaaagctg agttccctgc
     5161 cacctcgcgg agtataggat atgattcggc gctcgtgcgg agatgtgggt gaggtcgtga
     5221 aatagtgttc agacagtgca cacacctccc tattttaggc cttttgggcc acacaggtct
     5281 gagtaattcc acaggcctca cccgtcttcc tttcgccagt cccatatggc gcgacttaag
     5341 ccgtgccact acatggtcag actcccctct gatgtggtaa tgagctgcag tactttctcc
     5401 tcggttcctt tttcagcatt gatgtaaaca aaaaactgtt ctccatagga tgtaccccta
     5461 aactcccagc agaacgcctc gggcgtgtca actgtctcca ctgggatcac cgcaagacgg
     5521 gccgactcgg ttaccgtaag cgccggactg aggagttccc gtgcctcatc tgcatcgatc
     5581 gaaggtttgg gaagtcggcg catagtgtgg gaggttaggt aggccaatgc atcataggag
     5641 agaatatcac cattgtccaa agccaccttc accttgacct ggtccggata gatcaccaca
     5701 ccatcctgcg tgtatacgaa cgggacaata gagacatcac caacctcaga tgcaaatgtg
     5761 ggagtcatat tgaccattcc aactttgctt aagaaggcgc tagcacgctt gacagactca
     5821 gccagatcta tcgtcttttt gtcaagatcc cgcgccacgt tcatcagcac aacgtgtccg
     5881 cccttcttcg tcacgtcaac aactgcgtcc ggctttccgc tcctcttcgg ggcgtcaagc
     5941 cgcaccctgt agatgtcaat gtcccctttg gcctttatat tctcttccac cgttgcagaa
     6001 tgggatgagg cgtcaaatgg aacgaatctg cgagccacct ctttggcctg atcggcggtg
     6061 atctccgcgc cagtgattcc ccttggcctg atctgctcga tatgatccga gaaaggtccg
     6121 tcatatatca gagtcgggaa agtctgaacc tgttcgtcga ctctagtgaa gccgtcctta
     6181 accacatctg gagtagcgct tgtgcctggt ttcggtttca cttctgattt tgcctgttcg
     6241 gctactctaa cccaattcac cttacctgta gatacctgtg cctgcatgtc ggcgagtcca
     6301 cgcgccaggt cttccgattg cttcttcatg tcgacaagtt tgttccaatc atcctcgctc
     6361 atcacctgct ggttcgctac cttgcgcgca gttgtaaacg caaagtcgcc tacctgtgtc
     6421 aagaaggctg aagtccgcgc caaaaccttg tgggttacag gaaggttgtt cagattcgcc
     6481 tgcgcattgc tggcctcccg ccaaacatca gagagcgcac ccacgcggag tctgggagaa
     6541 ccggatgcaa ggctcttgga tagcgctact cggacattgt ctacatttcc cagaagatcg
     6601 aagaatgacc gaaaatactg gttccccacc gccacttcca gcctttgctt ggcggcccat
     6661 tgcgaatagg caaacacaag gctcacaatt acaattacta gtaggagcgc ctgtggccaa
     6721 ttgcgcatct tcccgcgcgc tccgcttgtc ccatcggtca tttgcattgt cccctttcta
     6781 cctggcaaac acgtgtttgc ctatttttac agcaatgctt ctgctccata tccatcctga
     6841 tacgggtttg gcagggttcc agaagaatac gcacccatat gtaggatcgt aaccgttcat
     6901 agcatctctt gcggcagata tcgcctgtct gctcggagag cgcctccata tcaatccgtt
     6961 tgagacagat tcaaatgctc caggttggaa tacacatcca gatattgaat tgggaaattt
     7021 gggactcgcc actcggttga gcatgacagc gccgacagct accatcccct ggtatggctc
     7081 accttcagcc tcggctgcaa tcaggcgggc aagaaggttg acgtcgccgc tacggctttg
     7141 agctcgacca atatgcgcaa ccgttccaat aacgacggca actgcaagca ctacaaagac
     7201 aaggatccgt gcgttgggca ttctcatgtt ggcatacctc cttccgatca aaaccccgct
     7261 ttgcatcagc gggcaaaaac atgcttaccg attttgacaa tgatattccg agtccaaatc
     7321 catccagata ccggctttgc ggggttccag aagaaaaggg atccgtaggt agggtcaaac
     7381 ccgtttatcg catctcgagc ggcgctgtac gcaatttgat ctggtgttct tcgccagtac
     7441 agcccgttgg acacagattc caaggcattc ggctgaaaga tcacagctga cagcgagtgt
     7501 ggaaacttgg ggtttttcaa tcggttcatt agaaccgccc ccactccaac ctggccacgg
     7561 taaggctctc ccctggcctc agcggctatc gcgcgggcaa ccaaatctac cgacgccgac
     7621 ctagatgcca ttggcgccgg cttgtaggcc gcgggcgcct cagcagccac cgtgtagccg
     7681 aagccaagag ccctccatgt ctgcggtcca acgaggccgt ccaccttcag cccgtttttg
     7741 cgctgaaatg ccaaaactgc tctgtgggta ttggcgccga aaacgccatc cacccaccct
     7801 cggtaataac cccaatcgga aagcttccat tgaacgagtc taaccgaacg ccctctggaa
     7861 ccccagtaca gcgtcggcct tgtctcggct ttagcagggg ttgcagcgca taaaagcacc
     7921 acaatgaaga tcatggcgcc tacagtcaaa cccataatcg gtgttttcag gttcatcgat
     7981 cagttacact cctgtcctgg ttggctcgca caacgtgctg gtattattct ctcagcactg
     8041 ttccatggat atgcacggac aaagacaagc cggaagctaa ggctgaaacc gcaatatagc
     8101 gcgtgcaacc acgctagagc atgaaaaaca atgtcatgcc ttacatgatc tgcagaaacc
     8161 ctcgcctagg tcccctggtg attgaacggg ccttccagcg ttctggacgt aggagaatag
     8221 tggagatatt atcagcttga cctaggcaaa gacttgatga tgcggaggat ggtcattaag
     8281 aatgatgcat acaatagggg ttgtcttcaa tctgaagaag gacttccccg gccttgtcgg
     8341 gaaagcaaac agcgttcccg atctggctgc ggaatgcgag gaagaggaga ctgttgacat
     8401 gctctcatcc tctctggcca agatcggtga gaaggtgatg ctgtttccgc acaaatctga
     8461 ccttttggag agattggacg aggcgcggcc tgacattgtc ttcaacatag ctgaaggctg
     8521 ggctgggcgg aatcgggagt cccttattcc ggcactgctt gagctgctcg agatcccgta
     8581 cactggctcc gatgccttaa cgctcggcat tgccctcgac aaagccatgg ccaaacgtgt
     8641 ggcatcgact gtaggtgtca ggactccccg ttacatcaaa gtcagttcca tagaggatct
     8701 gtcctccatg aatatggact ttcctgtatt cgtcaagccc aactacgaag gatcgagcaa
     8761 gggaatacgt aattcgtcgc gcgtcaggaa catggatgag ctgactgaaa aagtcaggtg
     8821 gatactggag acatattcac agcctgcgat tgttgaggag tttcttccag gaagggaatt
     8881 caccgtcgga atcatcggaa acggacgacc acacgtcctc cctataatgg aagtatgccc
     8941 cggcaatcgt gactcagccg attgcgaatt cgtctattca tacgaaacca agtcaggaaa
     9001 ccttgagcgc ttcagggttc ctgcgcctat tccagagcac ctggcatcgg agatcgcaga
     9061 tatggccgtc tccgtattcg aggtaatgga atgtcgggac gtggcaagga tcgacttcag
     9121 gctcgatgca gaagaacgac cgcatttcct ggagataaac cctctgcccg ggctctccaa
     9181 agtcagtttg cttccgcttc aggcccgggc ggctggaatg acgtatgacg atcttgtgaa
     9241 gtggattctc acatcagcct ggtcccgcta ctggcactag ctggccaatc aacatggcgc
     9301 tttcacaagc gaaaagagcg cgttcgtatt tcgctcacag cgatgcgaac gtgctctttt
     9361 attgtgttac aaagcgcccg ttcccctctg cgcttattac cccgttccat cggggcatac
     9421 taacgcaggc atttcatagc cagtctcttt gagaggagaa aagaacaggt atggataaca
     9481 gggttaggtg ttcggtaaac aactgccatt actggaatga aggcaactat tgccatgcat
     9541 cacagatcat ggtaacgtcc gattcaatcg caaatcaact tccagacagt tacgacgcgt
     9601 tgcaagcttc catagctgag ccaaggccgg ctgcccacat cggcgagact tgctgcaaga
     9661 cattcgctcc caagggaacg tcagatacga cggtacgctc ggatcgaact actcgaatgt
     9721 aataggagtc cctgcgttac accgggcggg accgtgcacg gtcccgccca actcgcatac
     9781 gaatggttgc ccacgctaat ggctgacacg cttttcgaaa ctgtgtatcg cctttacatc
     9841 atgaggaaat gcccacagat agggctgaac caaaacaggg ggcacccgcg ttctaagtag
     9901 gtctcgataa gtttctgcgc gaccttggag tctgtgctgc ctgcggttca aatctagact
     9961 gcttgatttg acaaagtgat tgctcaggta tgcgccaaaa ggcatttgag cgctccctga
    10021 agcagccaaa ccccttacgc caaatagtaa tcagtcgcgc aaacgccgcc ggaagactga
    10081 gctggccttt gaggcccgga caagaagtgt gtggcgatgg ccaaggtttg ctgcgaggaa
    10141 aatcagaggt tggcaacgaa cctggccaac ttctagtcac atccaattga ggcgaactag
    10201 ctgatacctt tttatggcta tgttcactgc ataaaccgac cctcactcgc aaaaagcagt
    10261 ccaacgcgtg tcgatagagc tactccgaat aagcctgttt tcggcaacaa ttcaatctac
    10321 gcccaccaag cgacttcgaa ctgagcttta tacacgtacc cagatgatgc tttcgatgcc
    10381 tacaatgtga tcaaatgctt ctacgtcgaa aagggcttac atcctatagt ccagcatgcc
    10441 tccacacgtt caacgccaca aacgatgcaa tgtgacttga acgctcacag aacttggctg
    10501 agcgcctcag ccgtaaccga acccgacttt cgcgaagacg cctacacatc tgggtggttt
    10561 attcggcctg cccaaacttg tctccggcac ggattcgcac ggctgcctaa gcctggcagc
    10621 ctatttgaga tttacctttt gacatgcttc tcacaaactc gttggagtcc tagcgtcccc
    10681 atacacgccg gcaattgcgg agtatctgga atcatctagc gaagacgtga ctgagttgat
    10741 gcaagcgcta gaggatgaaa ggggtaatgg caggcgtcgc ctctcaacat aagactacca
    10801 agggtcttcg tcagggctaa tgaaaaacgg acagctccgc caggtttgca atctcgacat
    10861 tacgctgggc gtggaggaag tcccctgatc atcggcgcac accggatctt tttgcaatct
    10921 catgaagcat taaagagagg ttgacgagat tttcgataac tcggtaggat aataggcctt
    10981 atacatgctg tatagaagca gcctgctgcc gcctatctcg gcaaatgagg ctcttttatt
    11041 gccaggaagg aagctgctct cgctaaattg tgtaactggc atgtgcgaga aatcggctac
    11101 aacgcagatg cctcgggatt ttgcgctctt tcaaccgcaa tacctgtaga gtataatggt
    11161 atggtattct atttatgagc ttcaagagtc gggggggaag cccatttgaa gcggacagaa
    11221 cacttgaagg atttctttgc caagtactgg tggcgctatg tcttaggaat actcactctt
    11281 attatcgtcg acgctctgca gcttgtggtt ccaaagatcc tcggaaatgt tactgatgca
    11341 ctccgcgaag gttcccttac accggaaggc ctcaaaaaac atgttctcat tattcttgca
    11401 atagcgtgcg gcatagcaat aggaagattc ctgtggcgca tcttcattat gggcactgca
    11461 aggttgctcg atttcgagct acgcaataag ctcttcgccc accttcagaa gctgcccgcc
    11521 cgctactaca ccgagcacaa gaccggcgag ctcatggcac atgcaacgaa cgatatccca
    11581 tcggtgaggc aagccttcgg gcaaggcttc atactcatta ccgactccat attcatgacc
    11641 acggcgacac tgataatcct gttcagaacg gtccccattc gtctggcaat cttcgctttg
    11701 cttccatttc cgctgttggc tgtgtctacc gcggctttta gcaagatcat aaatccacgg
    11761 catctagctg tgcacgaggc ctttggagaa ctctcggatc gagctcaaga gagcatagca
    11821 ggcatacggg tcacaaaatc ttttgtgcag gaagacgctg agattgcgaa attcatggct
    11881 gtagcccaga agaatgttga cgcaaacata gaccttgtga agatatgggg gctcatgtcg
    11941 cctttgagcg gcctggcaac tgcgctcgcc tacgcagttg tccttcgata tggttcgtca
    12001 atgataatgt accaacagat ctctctcgga gattttgtag ctttcacgag ctacctggcc
    12061 atgctggtat ggccaatgat agcgcttgga tgggtcataa acgtcatgga gcgcgggttc
    12121 accgccctcg acaggcttaa cgccgtgctg aacgaggtcc cggaggttgc ggatgagccc
    12181 ggcgcaattg agctagagga cgtcacagga accattgagt tcaggaatct gaccttctcc
    12241 taccgagacg gggtgcctcc ggcgctctcc gatatttcct tcaagataga cgcaggaaag
    12301 accctggcag tagtgggccg gaccggaagc ggcaagacga ctatagtcaa cttattgacc
    12361 agactctaca atccacctga aggttcagta ttcatagacg gtcacgacat ccgcaacgtt
    12421 acactgagct cgctccgcaa ccagatcgga tgtgtgccgc aagacacgtt cctgttctcc
    12481 tgcacgattg gggaaaacat cgccttcgca gatagggact tctcgcccga ggagatatgc
    12541 cactacgcaa agatagcaca tgtatacgat gacataatgg gcttccctga aggcttcgac
    12601 acaaaagtcg gcgaacgcgg agtaacgctg tcaggcggtc agaagcaacg gatctccata
    12661 gcgcgggccc ttataacaga gcctaagatc ctcattcttg atgactcgtt gtcagccgta
    12721 gacactcaga cagaggagag tatacttcgt gggcttagcg aattcatgca tgatagaaca
    12781 agcataatca tctctcaccg catctccact gtaaaggatg ctgacgagat aatagtgctt
    12841 gatgacggca aaataatcga acgtggaacc cacgcgtcct tgctggccca ggcagggctt
    12901 tacagtgaga tctatgagaa acagctgctg gaagaggagc tggccagcag aagctgaccg
    12961 ggggagaggt gagatgtaag tgaacggtta tgaggaagag gaacaaatca tcggaaaagt
    13021 gtacgattca cagcttatac ggcgactaat gtcgtacgcc gcaccatacc gcaaaacgtt
    13081 cgtgctagga ataatagtta tcatgataat cgcgggaaca gatctagtca ggccatacct
    13141 tatcaagact gctatggacg attacatctc tggatacgat gcccctatgg ctcgctatga
    13201 acctgaggcg gttcccacag gtattcgaga taatcgcatg atacattaca atgggcacgt
    13261 ctacgtccgc atcaaagact tgcccagcaa ccatgcggat gaatacgagc cggaagccct
    13321 tttccagctt ctgaacatct ccgatcagta tgttctcata tctggagcgc tcccgccgga
    13381 tcacggcccc gctcaattgg caacaacgga ttcaggccta gtgctcatcg tagacgaggt
    13441 cgaacacccg gcccaaataa tcgaaggcca cgagaatatc ggcatctttc gggagcaaga
    13501 ccttgccgca gtatcaagat tagccattat tctgctccta gtcggcgccc tggcattcgt
    13561 cctcaactac gggcaaacag tcctgctgca acgagccggc caacggataa tatatgaaat
    13621 tcgcgaagag gtattcacac atcttcagca catggatgta gcgttcttcg acacgaatcc
    13681 cgtgggccgc ctggtgacca gggtgacaaa tgataccgag actctcaatg agatgtatac
    13741 cagcgtaatc gttaacctct tcaaggacgt gttcatgctg gtcgggataa tcgttgtgat
    13801 gttccgtctc gatacgcagc tcgctctcct gtcccttgca gccttgcccc tgataatcgt
    13861 ctcgaccatc atcttccgta ccagggccag acaggcatac aggcaagtcc gaactgcgct
    13921 ggcgcggatc aactcgacat tggctgagaa catctctggc atgaagatcg ttcagatctt
    13981 caatcggcag caaaggaact atgacgattt cgtaaatgtc aatcgcgaat actatcgcgc
    14041 gggcattggt gagatgatgg tatatgcgat attcagaccc ataatggagc tcataggaac
    14101 gggcgcgcta gctctggtcg tatggtacgg cggcaggaac gtcatctcag ggactatgca
    14161 gtttggcatg gtttatgcct tcatcaatta cgtcagcatg ttcttcggcc ctataaacga
    14221 tctgaccgag aaatacaatc tcatgcaagc ggcgatggca gcatctgaac gcctattcca
    14281 gctgatggac accgagatca tggtaaaaga ccgcgagaat cccgtttctg tt
//
LOCUS       DAOSAI010000016        13533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3082_length_13533_cov_3.781273, whole genome shotgun sequence.
ACCESSION   DAOSAI010000016 DAOSAI010000000
VERSION     DAOSAI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13533
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3082_length_13533_cov_3.781273"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..118
                     /locus_tag="PLM83_01355"
     tRNA            43..118
                     /locus_tag="PLM83_01355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Arg,seq:cct)
     gene            376..945
                     /locus_tag="PLM83_01360"
     CDS             376..945
                     /locus_tag="PLM83_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70091.1"
                     /translation="MKKGRNAWIVAAVLCVAVVAVLIIGKGMKPDKKEANVENAGPPR
                     EKEVDAVEVMLPTELMTEDGLFLLEVPEGAIFELAADEDDVVWVYGPSGTRYKIRHED
                     VSAILKHRVSMDGYDDAELKQFGFQVAAARVAAVMQSAYPEEGTQLTVGMRTEWQDKV
                     EIEMATKSGISIPQYSLMVEWAVYKGWLD"
     gene            1030..2766
                     /locus_tag="PLM83_01365"
     CDS             1030..2766
                     /locus_tag="PLM83_01365"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOK70092.1"
                     /translation="MDYAAVYESWRTSSCVDEATKVELAGIENDVAEIEERFGRELGF
                     GTGGMRGKLGAGTSMINRYTVRRVTQAYASYLRECVEGSSEAGVVIAYDSRRMSRELA
                     EEAARVLAAAGIRAMVFDGIRPTPLLSYAVREMDAAGGIMITASHNPPEYNGYKIYGS
                     DGVQLLPEEASQIAARVSGIDDYLCFAAKPVAQVEGIDAEMDERYVSRVTELIEELVG
                     DMVSRRDREGLGVVYSPLHGTGYLLVPRALSDLGFAGLNVVREQSVPDGDFPTVKLPN
                     PEDPSAFELSIDRAMSMDVRNRPAIIIATDPDCDRVGVAVWDGEGYRVLSGNQVGVLL
                     ADFLIESAKMRGIELGECAIVKTIVTTEMVRALADGEGVHVIDTLTGFKYIGAHIDEL
                     EAKGRHFLLGLEESCGYLAGDFVRDKDGVIASALVASVAAWHMAHGRTLLDRLNELYG
                     THGYYYERLVSVPIAGDGGGVEALDALRQEMPDYIAGFPVVEVHDYLTGWTKKRGAGI
                     RVPIDLPRENCIQLVLSRGSKITLRPSGTEPKLKLYVAVRAGSQLKAEAKADAIVTTA
                     MRLLPISQEGYD"
     gene            complement(2879..3658)
                     /locus_tag="PLM83_01370"
     CDS             complement(2879..3658)
                     /locus_tag="PLM83_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70093.1"
                     /translation="MRFRRIAAATAIALIFAVTLAGCGGGGQKKLMSYFPDEEGYSWE
                     YEGTDGYVHRMTLDDIYEDSSTKEVTFTITGEIEGIEEEDDDDVDDVWSAFDLEEEEE
                     EEEEDDWSWSWDDEEDDDEDDWGWGSWWDDEEDTAATAGSTTLELRYEFTKDSVYEDI
                     IDGELALPHKFTRLQVLKTPIKKGTKWTYETPDWTEVEAEIIEVGKDDDKLDYVKVQY
                     TAELYEGTYTETRVYKKGLGLVEFTADNPDGSTFSYSVISW"
     gene            4290..5906
                     /locus_tag="PLM83_01375"
     CDS             4290..5906
                     /locus_tag="PLM83_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOK70094.1"
                     /translation="MLRGKVLLAECLAAALILCAAGVICHLGPGKTGVFETQLDSCWR
                     QFGQATVAASSGSGTLSTEGWGFETYVGYGDLLSVSTKAGWENEASGLFEIAGWSSYS
                     DVVAIVRREDLGDERITWLVSEPDGWFSGTVWFVDGPGRYSVSVATRPPKKASYIVVC
                     SFCVHNTSDEIPLAPIEYVGYGHDVTIDSPRQESNVVTDLLNVRGWSRYPRVRATVEN
                     METGEIRTYYTQTAQDGTFEFPVPLDVGYGPCEVILSSCQIDAETWRGAAAYRLISEE
                     PPLYVTQPAYQRGSILATERFKVRGIARGAESVILETVSYEGDAVLRRQEAQVAEDCW
                     EAWVDLPEGEDLFRLRIGRRLSGSEGENCEVNRSYTVARVKPRDDPAPTELWMTQSVS
                     MAIRELAESIVDGVNDTYERVRAVHDWVAGNIVYDVAGARNGRPGSGDAITTLRRREA
                     VCLGYSNLTAALLRAAGYRVWVVIGSADNGVSVLRHAWNEVEVDGRILSIDATWDAGC
                     IERGVFVRRLSGALFDPSPELLAFSHFAEI"
     gene            6542..7909
                     /locus_tag="PLM83_01380"
     CDS             6542..7909
                     /locus_tag="PLM83_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK70095.1"
                     /translation="MKSASVRDLLFPTTIAWKTYVIVIAASLVPALLAVALGGRELAQ
                     ARPEGYERELSSVCRLLDAELESRHGSFAHLLETEGALAAPREKQIQLLNAELQPIVD
                     MISVAYPDVEFGYYSKDLRAELAIHPMTDAVNIYKEVLPEEFNIYRTGRPEVLRPLVS
                     EAQRGHGGMAVQTCPLYDGDELVGHVWAKMPVRIEHKRLYTAGSLVGLAAVTAAVLLV
                     SSWYIMRGLGHWEERYALSRGAMANREAAAIVHEMRNPITVSKGLVQLVLTQETDMRK
                     RMWLSNIVRQLDHMNNIASDYLQYARPRPPRVSDVSVQELVEDLVENVAPYVEESGAI
                     MEVSSDCDDLKVRCDRDQVGQVLLNLVKNAVEAGLGEPGLRVAIRWRREGGTVIIEVA
                     DNGPGIPQENLSRIFDPFFTLKPKGTGLGLAISRTLIEQQGGSLAAHSSQAGGAVFSV
                     KLPRA"
     gene            complement(8071..8412)
                     /locus_tag="PLM83_01385"
     CDS             complement(8071..8412)
                     /locus_tag="PLM83_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HOK70096.1"
                     /translation="MPVDLIKRLSNESGMIKLAYEDKILNCRDCGQDFVFTAGEQEFY
                     DQHGFQNEPSRCPDCRRARRASSGSGNRQMYEAVCSACGKPAYVPFQPSGDKPVYCSE
                     CFAAQKSMRNR"
     gene            8831..9289
                     /gene="tadA"
                     /locus_tag="PLM83_01390"
     CDS             8831..9289
                     /gene="tadA"
                     /locus_tag="PLM83_01390"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOK70097.1"
                     /translation="MKQELLDVKFMSEAMAQAHEASLAGEVPVGAVVVMGDEVIARAQ
                     NRKESDKDPTAHAEILAIREAANRIGSWRLSGATIYVTLEPCPMCAGAIVQARLDRLV
                     YGAADPKTGAAGTIFDLPRDMRLNHWVSVTGGVMAEEAGALLEKFFSNKR"
     gene            9309..9399
                     /locus_tag="PLM83_01395"
     tRNA            9309..9399
                     /locus_tag="PLM83_01395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9344..9346,aa:Ser,seq:cga)
     gene            9438..9528
                     /locus_tag="PLM83_01400"
     tRNA            9438..9528
                     /locus_tag="PLM83_01400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9473..9475,aa:Ser,seq:gga)
     gene            9551..9815
                     /gene="ffs"
                     /locus_tag="PLM83_01405"
     ncRNA           9551..9815
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLM83_01405"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            9965..11695
                     /gene="dnaX"
                     /locus_tag="PLM83_01410"
     CDS             9965..11695
                     /gene="dnaX"
                     /locus_tag="PLM83_01410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOK70098.1"
                     /translation="MTYVSLYRRWRPQTFDDIVGQGHVVRTLKNAVSTGRISHAYIFS
                     GPRGTGKTSTARVLARALNCEKGPTPDPCGECRSCVAIRDGNAMDVIEIDAASNRGIE
                     EIRDLREKVVYAPTQGRYKVYIIDEAHMLTQAASNAFLKTLEEPPSHVIFVLATTEPE
                     NILETIRSRCQRFDFNRLSVSDLADHVSRVAAAQSISIDPDAAKLIARRADGSARDAL
                     GLLEQASAWSTHVTSESVLELLGTSDFDTLYNYADAARREEPGQLLSIIRDVVESGAD
                     MRQFLQDLVVHFRNLLVAKECPDLGGLLDAPEAVQASIAEQAAFFTRERIIEILDILS
                     EAEIQVRRSPNPRLLVELAAAKICAEPREDAVAQVGRAQKDTESFAGVQAAETRRKGL
                     EQAATLPDRKGSPGAGGSLDASGAGRRDGGEEAKPQGQAKGARGTGDARQEQNAQSGY
                     VGISEEEWNEALAHIKKANLVLEAFLKPVTAVCVDGDVATIMYDPSWTVHVQKASEPD
                     NKELIARALTDVLGRPVQCRVMAKAPQTKAKPSGEGPDGGGFGDYSDVPEVLEAIKLF
                     DPVEIKVIED"
     gene            11717..12034
                     /locus_tag="PLM83_01415"
     CDS             11717..12034
                     /locus_tag="PLM83_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOK70099.1"
                     /translation="MGGNMKKMMKQVQKMQEEMERVQQELAEERIETTAGGGVVRAVV
                     NGHQEVLEIHIDPAVVDPDDVDMLEDLVVAACNEALRKAKELAEQRLAKVTGNISIPG
                     LNF"
     gene            12061..12660
                     /gene="recR"
                     /locus_tag="PLM83_01420"
     CDS             12061..12660
                     /gene="recR"
                     /locus_tag="PLM83_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOK70100.1"
                     /translation="MLLYPEPVGRLIEELRRLPGIGQKTAQRLAFHILSMPEAEVKSL
                     SDALVQAKEKTRSCSVCGNFTDSDPCAICGDGSRDQSTICVVERPEDVIALERTREYR
                     GLYHVLGGAISPMEGVGPSDIRLRELLERLKSDVVCEVILATDPNIEGEATAIYIAKL
                     LKPLDIRVTRLAHGLPVGGDLEYADEVTLARALEGRRGM"
     gene            13108..13416
                     /locus_tag="PLM83_01425"
     CDS             13108..13416
                     /locus_tag="PLM83_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70101.1"
                     /translation="MDPRAIYTRLAGSFRAVFPSRSPAVEDYGRGWLKGEIRSVCAQL
                     EQADLVFNNVTEPELIDAAIFTMRACERRLGYLFKQAKALSLAESCHNEAETKSQNWC
                     "
BASE COUNT         2983 a         3220 c         4157 g         3173 t
ORIGIN      
        1 cgcaggccgc tggttcgaat ccagccgggc gcgccatcct ttggccctgt agctcagggg
       61 atagagcagt ggtttcctaa accattggcc gcaggttcga ttcctgccag ggccaccatt
      121 gttatggcga cgcggtggag ctttctgccg cgttattttt gtatgtggac aactgtgatc
      181 caatattgcc cgccttggca atgttttcga ttgttgccga ggagctagct tttgtggata
      241 gatctttcca tggcccaatt tcctgccggg ttcaggcggt tcgaccctct cacgagggga
      301 acaagtttgt cctctttgag cacgaagatg tggtaatatt caataaggcg agcactgtct
      361 ggtggagtga tgcttatgaa aaaaggaaga aatgcttgga ttgtggcggc cgtattgtgc
      421 gttgccgtgg ttgctgtgtt gatcataggc aaggggatga agccggataa gaaggaggcg
      481 aatgtggaaa acgctgggcc tccgagggag aaggaagtag atgctgttga ggttatgctc
      541 cctacggagc tcatgacaga ggacggcctg ttcctgctcg aggtgcccga aggcgccata
      601 ttcgaactgg ctgctgatga ggacgatgtg gtatgggtat atggcccaag tgggacgaga
      661 tacaagatta ggcatgagga cgtttcggcc atactcaagc atcgggtatc tatggatggg
      721 tacgacgacg cagaactgaa gcaattcggt tttcaagtgg ctgcggctcg tgtggcggcg
      781 gtgatgcagt ctgcttatcc tgaagaaggg acgcagctta ctgtaggcat gagaactgaa
      841 tggcaggaca aggtggaaat tgaaatggcg acgaagagtg ggataagcat cccacagtat
      901 tcattgatgg ttgagtgggc agtttacaag ggctggcttg attgaagtcg gaagttgtcg
      961 aaggaacttc ggggcttttc ggagaaccat tcgctatgcg agcaaggtag ccatatgggg
     1021 tggttattga tggactatgc agcggtttat gagagctggc gcacgtcgag ctgtgtcgat
     1081 gaggcgacga aggtagaact tgcaggcata gaaaacgacg tcgccgaaat cgaggagaga
     1141 tttgggcggg aacttggctt tggaaccgga ggcatgcggg gtaagctggg cgctggcacg
     1201 agcatgatca acaggtatac ggttcgtcgt gtcactcaag cctatgcttc ataccttcgc
     1261 gaatgcgtgg aagggtcgag cgaagccgga gtcgtgatcg cttatgattc taggaggatg
     1321 tcaagggagt tagctgaaga ggccgcacgg gtgcttgccg cggccggcat cagggcaatg
     1381 gtgtttgacg ggattaggcc tactccgttg ctttcctatg cagttcgcga aatggatgca
     1441 gcaggcggga tcatgataac agcaagccac aaccctccgg agtacaacgg ttacaagatc
     1501 tatggttctg atggggtgca gttgcttcct gaggaggcta gccagatcgc agccagggtt
     1561 tccggcatag atgattatct ttgctttgct gctaaacctg tggctcaggt agagggtata
     1621 gacgctgaaa tggatgagcg gtatgtcagc cgtgtcacag agctcatcga agagcttgtg
     1681 ggggatatgg tttccaggcg cgaccgtgaa ggacttggtg ttgtatacag ccctcttcat
     1741 ggcacaggct acttgctcgt tccacgggcc ttgtcagact tgggttttgc ggggttgaac
     1801 gtggtacgcg aacaatctgt gcccgacggc gactttccaa cagtgaagtt gccgaatccg
     1861 gaagatccgt cggcttttga attgtccatt gaccgcgcca tgtcgatgga tgtccggaac
     1921 cgccctgcga ttatcatcgc aactgacccg gattgcgacc gcgtgggtgt ggcagtatgg
     1981 gatggcgagg gatatcgggt tctttcaggg aaccaggtcg gtgtcttgct tgcggatttc
     2041 ctcattgaaa gcgccaagat gagaggcatt gaactcggcg aatgcgccat agtgaagacg
     2101 atagtcacta ctgagatggt gcgggcgctt gcagacggag agggtgtgca tgtgattgac
     2161 acccttactg ggtttaagta tattggagcg cacatagatg aacttgaagc gaaagggcgc
     2221 cattttctgc tcgggcttga ggagagttgc gggtatcttg ccggcgactt cgttcgtgac
     2281 aaggacggag ttatcgcatc ggccctggtt gcatccgtag ctgcgtggca catggcccat
     2341 ggccgtacgt tgttggaccg cttgaacgag ctctacggga ctcatggata ctattatgaa
     2401 cgccttgttt cggttcctat tgctggagat gggggcggag tagaggcctt ggatgctttg
     2461 cggcaggaga tgcccgacta catcgctgga ttccctgtgg ttgaggttca cgattatctc
     2521 actggttgga cgaagaaacg tggggcgggc atcagggttc cgatcgacct tcctagagaa
     2581 aactgcatac agcttgtgct ttcgcggggc agcaagatca cgctgaggcc gtcaggcacc
     2641 gagcccaagc tcaaattgta cgtggcggtt cgtgctggat cgcagttgaa ggctgaggcg
     2701 aaagctgacg ctatcgtgac gactgctatg aggctccttc cgatatcgca ggagggctat
     2761 gattgaggga gggaccagac tcggcttcct gaggcattcg gaaagaaata taaatgaaac
     2821 cacaaggggg cggcatatgc cgccccctgt gaactacgtt gactgtgtgt atgttagatt
     2881 accagcttat caccgaatag ctgaacgtgg agccgtcggg attatcggcc gtgaactcca
     2941 caaggcctag acccttcttg tataccctgg tctctgtgta ggtgccttca tacagctcgg
     3001 ctgtgtactg aaccttaaca tagtccagtt tgtcgtcgtc cttgccgact tcgatgatct
     3061 ctgcctcaac ctcggtccaa tcaggggtct catacgtcca ctttgtgccc ttcttgatgg
     3121 gggtctttag aacctgcaac ctcgtgaact tgtgtggaag cgccagctcc ccatcgatga
     3181 tatcttcgta gaccgaatcc ttagtgaact cgtacctcag ttccaatgtg gtcgaaccag
     3241 ctgtggcagc agtgtcctct tcgtcgtccc accagctgcc ccaaccccaa tcatcctcgt
     3301 cgtcatcctc ttcgtcgtcc cagctccagc tccagtcgtc ctcttcctct tcctcttctt
     3361 cctcttcgag gtcgaaggcg ctccagacat cgtccacatc atcatcgtct tcttcttcga
     3421 ttccttctat ttcgccggtg atggtaaagg tgacttcttt agtggatgag tcctcataaa
     3481 tgtcatccaa agtcatcctg tggacatatc cgtcggtgcc ttcatactcc catgagtatc
     3541 cttcttcatc ggggaagtag gacatgagct tcttttgccc gcccccacca caacctgcta
     3601 gcgttactgc gaaaatgagg gctatggcag tagccgctgc aatcctcctg aatctcatac
     3661 acctatcagc tcctcgtata tttgctcaag ccgcccagat aggccagctg gcggcctgga
     3721 aaccaacgtc catcttccag gttttgattt aacgcagcgc cgtcatagga aggatcacga
     3781 attttcactc taatcagtca ttcgtgtctc tatgtctacc aggcggacgg gagttgccct
     3841 ggacagatgg aagttgggca taagtaccct tcaatatata taaataccac ggaccgggac
     3901 ttttgtccac cgccgcccgg cagatgggct aaccacatta tattacatat ccggcatttc
     3961 tggtagacct gccctgcttt tcagaaggat gggagcagca tggacggtct acgctggcgc
     4021 attcgagatt gcgccctttt tcgtatccag acagtatgct ccttcagcgt tcgacaggac
     4081 cttcggcaag gttcgtgatt ttcgtcgctg ccggtatcgc ctgggaacga gaacctccgt
     4141 aaaatacctc ctactcttga agacaggtcg aaatcatggg aatttcgctc tgggcccgca
     4201 gatagaaact tacatggttc gagagggcat ggctatagga tatgtggtga aaatgacatg
     4261 gcaaagcgct tgttgcaggg ggtgcgatga tgctcagggg aaaagttctc cttgcggaat
     4321 gtctcgcggc agcgctgatt ttgtgtgcgg caggagtgat ctgccactta gggcccggaa
     4381 agacgggcgt ctttgagacg cagttggatt catgttggcg tcaatttgga caggcaactg
     4441 ttgctgcttc ctcgggatct ggaacgttgt cgactgaagg atggggcttt gagacatatg
     4501 tggggtatgg ggatctgctc agcgtgtcta cgaaagccgg atgggaaaat gaggcttcgg
     4561 gcctttttga gattgccgga tggtcgagct attcagatgt agttgcgatt gttcgtcgcg
     4621 aggatcttgg agatgagcgc attacgtggc tggtttccga gcctgacggc tggttttcgg
     4681 ggactgtatg gtttgtggat gggcctggaa gatactccgt gagcgttgcg acgcggccgc
     4741 ctaaaaaggc atcatacata gttgtttgca gtttctgcgt gcacaacact tcggacgaga
     4801 ttccgctcgc ccctattgag tatgtggggt acggccacga tgtgacgatc gattctccga
     4861 ggcaggaatc gaacgtcgtg actgatcttc ttaatgtgcg gggttggtcg cggtatccac
     4921 gggtcagggc gacggtcgag aacatggaga ctggcgagat ccggacttac tacacccaaa
     4981 ccgcgcagga tggaaccttc gagttccccg ttcccctcga tgttgggtat ggcccttgtg
     5041 aggtcatact gtcttcctgt cagatcgatg cggagacatg gcgaggggct gccgcatacc
     5101 gcttgatatc cgaagagcct cctctatacg tgactcaacc cgcataccaa cgagggtcga
     5161 tactggcgac tgaaaggttc aaagtccggg gaatagcgcg tggcgccgag agtgtgattc
     5221 tggagaccgt gtcctacgaa ggagatgcgg tcttgcggcg ccaggaggct caggtggccg
     5281 aggactgctg ggaggcatgg gtagacttgc ctgaaggtga agatctcttc aggttgcgca
     5341 tagggcgtcg tttgagcggg tcagagggcg agaattgcga agtgaaccgt tcatacacag
     5401 tggctcgggt gaagccgcgg gatgatccgg cgccgacaga gttgtggatg acgcaatctg
     5461 tgagtatggc tataagggag cttgcggagt caattgtgga cggcgttaac gatacctacg
     5521 agcgtgtgcg agctgtccac gactgggttg ccgggaacat cgtgtacgac gtggcgggag
     5581 cccggaatgg ccggccgggc tctggggatg ccatcaccac gcttcgcagg cgcgaagctg
     5641 tgtgccttgg atattcgaac cttacggcgg cattgttgcg ggctgcaggt taccgtgtgt
     5701 gggttgtgat aggcagtgcc gacaatgggg tgtccgtgtt gcgacacgct tggaatgagg
     5761 ttgaggtgga cggaaggatt ctatcgatcg atgcaacatg ggacgccggc tgcatagaga
     5821 ggggagtctt tgtgaggcgg ctgagtgggg cattgtttga tccgtcgcct gagctcctgg
     5881 ctttcagcca tttcgccgaa atctagtgcc actggcacag tgcttggatg gagaatgcac
     5941 ggattgttgc tcggagcgaa aaaaggatgt aatcaacttg tagcttcaat tgttatttgg
     6001 aaagtaaatg cccgacagcc aaagatacaa gcgcagtgta tgcacggggg tttggtagta
     6061 ggcgctgagc agtataaata tgcctgatag tgcatgaaca gcatgggctc cgtgggagga
     6121 tgcgacatga cgggtagaaa agctacggga acagttttgg cgttgccctg catcgcgtca
     6181 ccatgcttgc agcggtagct catggcgcga ttgctgtgaa ggaggatggc gaaaaggcaa
     6241 tatcatcttc gggcgagatt ggctatgcca tcatacggag caaagatggt tcaaatgccc
     6301 atgctgttat ggccaccgca tcgttttgac tggcaagccg cttgtagaaa ggggataaga
     6361 cacgcagcga cagcagtgcc atcatccttt gtcccctacg gctgggtgca acagcttcat
     6421 tatgtggcaa cgagtgggat ttcccgtcag tcatgaggag tgaagaatct agggcagcca
     6481 tatggttgcc ctgttgcacc catactgatt tggacaaggt tttcgcgggg agtgtagtgc
     6541 attgaagtct gcttctgtgc gggatcttct ctttcccacg actatagcgt ggaaaacata
     6601 tgtcattgtc atcgctgcgt cactggttcc agcgctgctt gctgttgcct tgggtgggcg
     6661 agaactggcg caagctcggc ctgagggata cgagcgggag ctatcctccg tatgcagact
     6721 gctagatgcc gagcttgaat cgcgccatgg atcctttgcg catcttctgg agaccgaagg
     6781 cgctctcgcg gcgcctaggg aaaagcagat tcaactgcta aacgcggaat tgcagccaat
     6841 agttgacatg atatctgtgg cctatcccga cgttgagttt ggctattatt ccaaggacct
     6901 tcgggccgag cttgccatac atcccatgac cgatgctgta aatatataca aagaagtgct
     6961 acctgaggaa ttcaacatat atcggaccgg cagacctgag gtgctgcgtc cattggtatc
     7021 tgaggcccaa aggggtcatg gcggcatggc agtgcagaca tgtccgctat acgatgggga
     7081 cgagcttgtt ggccatgtgt gggcgaaaat gccggttcga atagagcata agaggctata
     7141 taccgcaggc agccttgtgg gacttgcggc cgtaaccgct gccgttctct tggtttcatc
     7201 atggtacatc atgcgcgggc tggggcactg ggaggagcga tacgcgctca gccgtggtgc
     7261 aatggctaat cgcgaggctg cggctattgt tcacgagatg cgcaacccaa taaccgtctc
     7321 caaaggcctc gtccaacttg tattgactca agagactgac atgagaaaga ggatgtggct
     7381 ttcgaatatc gtccgccaat tggatcacat gaataacata gcttccgatt atcttcaata
     7441 tgcccggccc aggccgcccc gagtatccga tgtttctgtg caagagcttg tagaggatct
     7501 tgtggagaat gttgcgccat atgtcgaaga atcgggcgcg atcatggaag tatcgtctga
     7561 ctgcgatgat ctcaaggtgc gatgcgatcg ggatcaggta ggccaggtat tgctcaacct
     7621 ggtgaaaaat gcagtggaag ccggattggg cgagcctggg ctgagggttg ccattcgctg
     7681 gcgccgggag ggaggaaccg tcattatcga agtggccgat aatggcccag ggattcccca
     7741 ggagaacctt tcccgcatat tcgacccatt cttcactcta aagccgaaag gcacaggcct
     7801 tggccttgcg atatcgagga cccttattga gcagcagggg ggatcccttg ctgcccatag
     7861 ttctcaggca ggaggcgccg ttttctccgt aaagttgccg cgggcgtgat cccgggaaga
     7921 tttgcttgct tcccggcagg cagaaaagtg cagtacgcct tgtgtatctt cagctgaaca
     7981 acggagaaag gaaagagtcc cggaagtatc ccgggactct ccatgcctac gtcgaatgct
     8041 tcatattcct aaacgagctt ttcgagttgc ctacctattc ctcatcgact tctgcgcagc
     8101 gaagcactcg ctgcagtata ccggcttgtc gccgcttggc tggaatggaa cgtaggctgg
     8161 ctttccacag gcggagcaga cggcctcata catctggcgg ttaccagaac cactagaggc
     8221 cctgcgggct ctcctgcaat ccgggcacct cgacggctcg ttctgaaatc cgtgctgatc
     8281 gtaaaattcc tgctctcccg cggtgaacac aaaatcctgt ccacagtctc tacaattgag
     8341 gatcttgtct tcgtatgcca acttgatcat ccccgattcg ttggatagtc tcttgatcaa
     8401 gtcgacaggc attagaatgc gtgcgcttga acaacagcca tatatatctt aaagcctttc
     8461 cggcgaactt gcaatatgct gccaacaaaa gtattgtctg acgcttcgtg cccacgtgag
     8521 tgtcaagccc acttttgcct ggtagagctg ggggttgtcc gagaacctcg ggaaattgtg
     8581 gcgaaaagaa acttcccgct cgatacctta tgtatggcca ttgttcactc ttaaccatag
     8641 atttgctgcc tgatctgagc ttctgtagga aataatgcac cgcagctttg accgattttc
     8701 caggtcctgt gcgccgatta ttgcgtttgc cgaagatgcc cgttcgccgg gagggaatgc
     8761 ggaggtagtg ttgatttacc ctcatcggta tggtaacata tgtggcgagc aaaggatgct
     8821 tcatagaact atgaaacaag aattgcttga tgtcaagttt atgagcgagg ccatggccca
     8881 ggcgcatgag gcgtcgttgg ctggcgaggt gccggtcggc gcggtagtgg ttatgggcga
     8941 tgaggttatc gcgcgcgcgc agaaccggaa ggaatcggat aaggatccaa cggcgcacgc
     9001 agagatactt gcgatccggg aggccgccaa ccgcatcgga tcatggcggc tttcgggggc
     9061 aaccatttac gtgacgctgg agccatgtcc catgtgtgcg ggtgccatag ttcaggcgag
     9121 gcttgataga ttggtttatg gagcggctga tcccaagaca ggggcggccg gtactatatt
     9181 cgacttgcca cgcgacatga ggctcaacca ctgggtgagt gtgacaggcg gggtcatggc
     9241 ggaagaagcc ggcgcgttgc ttgagaagtt cttttcaaat aagcgttgat atttttcata
     9301 tagcttgcgg agagatggct gagctggtcg aaagcgctcg cctcgaaagc gagtaggcgg
     9361 ggaaccgcct cgtgggttcg aatcccactc tctccgccat agattctgag gcctatttct
     9421 ggggctttcc atattgcgga gaggtggctg agttggtcta aggcgctcgc ctggagagcg
     9481 agtaggcggg gaaccgcctc gtgggttcga atcccaccct ctccgccatt tttgttcata
     9541 ttgactttgg ccgtgctaga cggggaggta gcggtgccct gtacccgaaa accgctatag
     9601 cggggttgac ttcccaccct aggcctgcct tctttggggc ctgcccgacg caagtggtgt
     9661 tgagggctgg gtcctgcgca acgaggctct gtgaaccccg tcaggaccgg gaggtagcag
     9721 cggtaagcag ataccctcgt gtgccgcggg agcgcctggt cggagctggc tacgtcggta
     9781 ccgtctgggg aagcttgtcg aaggtgggtg cacggcctct acatcgtcag gatggtccgg
     9841 ctgggccatc cttttttgat gtggaggaca aagagagacc tggagcgaat actacttgag
     9901 gcatgttttg ctgagcctgc tttctagggc ttgcaaaggc aaatcggatt ttggggggct
     9961 accggtgact tacgtttcgc tctaccggcg atggcgtccg caaacgttcg acgatatcgt
    10021 tgggcagggc catgttgtca gaaccttgaa gaatgcggtg tcaacggggc gcatttccca
    10081 tgcctatatt ttctcagggc caaggggaac tggcaagact tctactgcaa gggtgcttgc
    10141 tcgcgctctt aactgcgaga agggaccaac gcctgatccg tgcggagagt gccgttcatg
    10201 tgtggcgata cgcgacggaa acgctatgga cgtcatagag atcgatgcgg cttccaacag
    10261 gggcatcgag gagatcaggg atctgcgcga gaaagtggtg tacgcaccta ctcagggcag
    10321 gtacaaggta tatatcattg acgaggccca catgcttaca caggctgcgt ccaacgcttt
    10381 tctaaagacg ttggaagaac cgcctagcca tgtcatattc gtcttggcca cgacagaacc
    10441 tgagaacatc ctagagacca tcagatcgcg ctgccagcgt ttcgatttta acaggctgag
    10501 cgtctccgac cttgcagatc acgtttcccg ggtggccgca gcccagtcta tatctatcga
    10561 ccctgacgcc gcaaagctca tagccaggcg cgctgacgga agtgcgagag acgcgttggg
    10621 ccttttggaa caggcatctg catggtcgac ccatgttacg agcgaatcag ttctagaatt
    10681 gctcggaacg tctgatttcg acaccctata caactacgcc gatgctgccc gcagggaaga
    10741 gccgggccag ctgctttcga tcatccgcga tgtcgtggag agcggcgctg atatgaggca
    10801 gtttctgcag gacctggtgg tccattttcg taacttatta gttgctaaag aatgccccga
    10861 cctcggcggg ttgcttgatg cgccagaagc ggtccaggcg agcattgcgg agcaggctgc
    10921 cttttttacg cgcgagcgta taatcgagat cctggacatc ctcagtgagg cagaaattca
    10981 ggtgaggcgt tcacccaatc cccgtcttct agtggaactt gcggcggcaa agatctgcgc
    11041 cgaacctcgc gaagatgcag ttgcgcaggt cggcagggct cagaaagata cggagtcttt
    11101 tgcgggggtg caggctgccg agacgcgtag gaagggcctg gaacaggccg cgaccctgcc
    11161 tgacaggaaa ggatcgccgg gtgcaggcgg cagtcttgac gcttccgggg cgggcaggag
    11221 ggacggcggg gaggaagcga agcctcaagg acaagcgaaa ggtgcccgag gcaccgggga
    11281 tgcgagacag gagcagaatg ctcaatccgg atatgtaggg atctctgagg aagaatggaa
    11341 tgaggctttg gcgcatataa aaaaggctaa cttggtgctg gaggcctttc tgaagcctgt
    11401 aacggcggtc tgcgtagacg gtgatgtggc gacaatcatg tatgatcctt cgtggacggt
    11461 ccatgtgcag aaagcctctg agcccgacaa taaggaattg atagcgaggg ccctcacaga
    11521 tgtgctgggg aggccagttc aatgtcgggt aatggccaag gccccgcaga caaaggcaaa
    11581 gccttctgga gaaggtcctg atggcggcgg attcggcgat tactccgatg tcccagaggt
    11641 attggaagcg ataaagctgt tcgatcctgt ggagataaag gtgattgaag attgaataga
    11701 gcggggaggg gacgagatgg gcggaaacat gaagaagatg atgaagcaag tgcagaagat
    11761 gcaggaagaa atggaaagag tccagcagga gcttgcggag gagcggatag aaaccacggc
    11821 cggtggaggt gtggtgagag ccgttgtcaa cgggcatcag gaagtgctcg agatccacat
    11881 agatccggcg gttgttgatc ctgacgatgt ggatatgctt gaagacttgg ttgttgctgc
    11941 gtgcaatgag gcgcttcgga aggcgaagga attggccgaa caaaggcttg caaaggtaac
    12001 gggcaatatc tccattccgg gactcaactt ttaggctcta gccgtcgggg gaaatcagat
    12061 atgttacttt atccagaacc agtgggaagg ctcatagagg aactgaggag gcttccgggc
    12121 ataggacaga aaacagccca gaggcttgcc tttcatatcc tctctatgcc tgaagcggaa
    12181 gtgaagagcc tttcggatgc gctggttcag gcgaaggaga agacgcgaag ttgctctgtg
    12241 tgtggaaatt ttactgattc tgacccgtgc gccatctgcg gagatgggtc tcgcgaccag
    12301 tcgaccatat gcgtggttga acggcctgaa gacgttattg ccctggaacg cacgagggaa
    12361 taccgaggcc tgtaccatgt cctgggcggc gccatttctc cgatggaggg tgtggggcct
    12421 tcagatattc gcctccgcga actcctcgag cggctgaaat ccgatgttgt gtgcgaggtg
    12481 attctcgcta ctgaccccaa tatcgaaggc gaggccacag cgatctacat cgccaaactt
    12541 ctaaaacccc ttgacatcag ggtcacgcgc cttgctcacg gacttccggt aggcggggac
    12601 ttggagtatg ccgacgaggt caccctcgcc cgcgcgcttg aaggaaggcg cgggatgtag
    12661 caaggccttt ctcgatcgac caagtttctc gctgttccgc gttggccagg ccgtgtgcgg
    12721 tggcgttttt tcatagttca agccattgag taatagggct ttgccaggtg attcgagggt
    12781 ccttgccggc cacgcgtatt acgaaacagc tcagttggcg aagcaatgtc gtgggagaac
    12841 acgaatgcgg ggagtcggaa gaaatggaat taggcctaga tagggcgggg tggaagcctg
    12901 aacgtgatcg acggcatcgg gccgggcaga cgtgatgcga tccttgtaga ttatccacct
    12961 gatacgtgtc actttgattc tacgtgtttg cggtagagac aatggatttt ggacatgttc
    13021 cataggcata ctatctaaat ggacggcgcg aactatatta tctgtgattg ttccgccacc
    13081 gacattgcga agagtgaggt gtgcactatg gacccaaggg cgatctacac gcggttggcc
    13141 ggctcgttcc gggctgtatt cccgtctcgg tcacctgcgg tcgaggatta cgggcgggga
    13201 tggctcaagg gggagatacg ttcggtatgc gctcaactag aacaggccga tctcgtattt
    13261 aacaatgtta ctgaaccaga actgatagat gctgccatat tcactatgcg tgcgtgcgaa
    13321 cgcaggctgg gatatctgtt taagcaggca aaagcactta gtttggcgga aagctgccac
    13381 aatgaggcag aaacgaaatc gcaaaattgg tgttgacatg ttcgagctag ggtggtaaga
    13441 tagcaaatgt cgccccgctg cggcggggga aaatcgatgc ggcgctaaag ccgcggatgt
    13501 tccttgaaaa ccaaacagcg tgaagcttga taa
//
LOCUS       DAOSAI010000017        12812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3294_length_12812_cov_3.917692, whole genome shotgun sequence.
ACCESSION   DAOSAI010000017 DAOSAI010000000
VERSION     DAOSAI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3294_length_12812_cov_3.917692"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..4152)
                     /locus_tag="PLM83_01430"
     CDS             complement(100..4152)
                     /locus_tag="PLM83_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOK70102.1"
                     /translation="MKSKRKVLFIFASLALVLFVGTLVSTSSQAQISAYDYILRGEQY
                     LAQGAANEAIGQFEQAIYIEPTNTRAHFGLGCAYEMRSKAARVFNYRMELFTEPDSVR
                     KLGFPPLSEQGYQDVERAIQAYSKAALYSPPYAEGHYRQGVLHFISGRPGDAKVALER
                     AVAQDRTAVDPAVLLAYIYLEEGNIQAATAQIDRIISATGDRLNPVILGVSAVLEYRS
                     GRYDQAEQLLLQATSRSGAPAYLYKRLGDAMAARGLHTQALDAYRQAVQRDPESILAN
                     DAIGNIYRLSGQSAQAVQCYSAAASANPNLLRARYGLGSALLESGSYAQALQTFSELV
                     DALPTYGSDNALLLKGIAQWKTGNANAALATVNHALGINAENRIAAIYKERIEREMGG
                     YGVSPLGVQPKSIVAEGSKPDKPFSGIVINDGAEYTNSRTVRLAVYSDYSTVAFSNDN
                     STWSSWFSKPEPYGEFTWELTPGDGTKRVYVKFRGLFGIGIPGESGTIVLDSTPPVAT
                     ARLESMQTGGYGGYDGRTRVVLSANDPSGIVGFWMSYDGNDWRWFDWYGSDFAISLPA
                     GVGATPRVRFSVVDGAGNHASIEAVMTAPPSLDTQPPRIYGVQATRGPLDSAIITWTT
                     DEPADSYVEYWAYGVPQNRVGTSMLATSHSVTLSNLRAGTTYYYRVISTDAAGNRGQS
                     QDLTLVIQPVDTTPPVISRIEAKATGLDSVSITWTTDEPADSYVEYWAYGVPQNRVGT
                     SVLSTYHAVTLSNLRSGVTYYYTVTSTDAAGNRARSSEMSLRIQPPDTTPPTVRVQIN
                     GGAQYTKSRTVQLEITAQDDSGGPLEMRIWDDKSSYGSWRAYSDRVTWELTTGDGRRT
                     VYVKVRDAAGNEAQAQAVITLDTEPPIIRSVQTKNIGPDSAVITWSTDENSDSWVYYG
                     TYSPNLVMGQYDFVTSHSVALYGLSPNTDYYFRVRSADAAGNMAESSTLTFRTSQLGD
                     RNPPTGSIAINNGAQYTRYYDVTLSIAAKDDSPGPIEMRLREGSGNWTGWMQFQQAMT
                     YRLSPGDGNKTVYVEFRDIYGNQSRTYSASITLDTRPPKITNVRTANITANSATITWN
                     TDEPATSTIEYGLTGTTMKDAAGDKSVSSSEASSPAGDVGARSVIITPVVPSGSLKTS
                     HSVTLTGLRDNTTYYYQVVSKDAAGNVSVASGFSFTTGSVAPPPPIGTVNVNWAAAAN
                     GGRASASSYAPASSDGPARPASMAIDGNMKTYWEASGPITGGKTEWLEVEFSASRVIN
                     TIRTSSDVGNYPYSMTVEVEENGRWIVVGSFTKPSEYQKYMKRSGDVVSFEFQFTAMS
                     VSKVRFVVARVSNPLNALQFYEVEAFNTGR"
     gene            complement(4291..5583)
                     /gene="asnS"
                     /locus_tag="PLM83_01435"
     CDS             complement(4291..5583)
                     /gene="asnS"
                     /locus_tag="PLM83_01435"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOK70103.1"
                     /translation="MQSVRIADIREHDGEEVTIKGWLYNKRSSGGIHFLIIRDGTGFM
                     QAVAVRGEIPDDQFELCNNLTQESSILVTGRIRADKRAAGGFEMSATGVELVQLAEEY
                     PITLKEHGVDFLLDHRHLWIRNPRQHAALLVRDEVISAAEDWLRSNGFVKIDAPILTP
                     AACEGTTTLFETDYFGEKAYLSQSGQLYNEAACMAFGKVYCYGPTFRAEKSKTRRHLI
                     EFWMIEPEMAYATQEDNMVIQEEFLSYIVQRCLAKRRQELITLERDLSKLECIVPPFP
                     RLSYDEAVELLHGEGKEFEWGDDFGAPDEATISMKYDKPVFVTGYPTKVKAFYMQPDP
                     DRPEIVKCSDLLAPEGYGEIIGSSERIHDLDLLAARMREHNLPEEAYGWYLDLRRYGS
                     VPHSGFGLGIERTVAWITGSEHVREMIPFPRLINRIYP"
     gene            complement(5679..5951)
                     /locus_tag="PLM83_01440"
     CDS             complement(5679..5951)
                     /locus_tag="PLM83_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70104.1"
                     /translation="MKDIAPVGPRKTVMFFSRGPRLFWDRGREERVLCVTADRALSGA
                     RLILYSGNGQALHGHIFAAAQSFAAYAYLDVAYRCDPSGGEDRSDP"
     gene            complement(6321..7529)
                     /locus_tag="PLM83_01445"
     CDS             complement(6321..7529)
                     /locus_tag="PLM83_01445"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOK70105.1"
                     /translation="MSTKIKRCSLLAVSLIMVMVVSMISSESALAEYDVKSIIINPNP
                     PYQTSIWTDKSQYNPGERLNVYFRTSKDAYIYIFDIDTVGNVSLIFPNIYSEYNYKRA
                     GQTYSLPDNSRYNLTIGGPAGVEQLVLIATPSQIRDVEWLRRSLEQGSFGPQLNVNIS
                     ADRFMFEVKSISITPNFGQDWSSAVATFTVGSVVTPPPPPPSVKYGTLSITSKPSGAT
                     VFLDGVDQGTTPLTITGVSYGVHDIAIMKKNYYTYSRQITVNSPNPYRIDATLVKLPT
                     SSDWGDEVQLVNKSMLVKWPQTGPFTETFTFRGSTGKVVINADPILGMLSRVTGSITA
                     NAVGPVEFMQINPSGPDAPWPGRVYERIQYPFRVRVTVEDYSITTGLVFGIEYIDYVR
                     LRLEIWHIGN"
     gene            complement(7561..9039)
                     /gene="guaB"
                     /locus_tag="PLM83_01450"
     CDS             complement(7561..9039)
                     /gene="guaB"
                     /locus_tag="PLM83_01450"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOK70106.1"
                     /translation="MHNPDKIREALAFDDVLLVPGISEVLPANVDVRTEITDKIMLNI
                     PLMSAAMDTVTEARMAVALAREGGMGIIHKNMSIEEQALEVDKVKRSEHGIIVDPIFL
                     SPEASVREALEIMERYHISGVPITEDGTLVGILTNRDLRFETDYDQPVSNVMTKDGLI
                     TAPVGTTVDEAIEILKVHKVEKLPLVDEEFKLKGLITIKDIKKTRKYPNAAKDEHGRL
                     RVGAAVGVSVGTMDRVAALIDAGADMIVVDTAHGHSRGVIETVKAIRANFGDIDIMAG
                     NVATGDGVKALAEAGANCVKVGIGPGSICTTRVVAGVGVPQFSAILECAAAAAEAGIR
                     IVADGGVKFSGDMTKALAAGAHVVMVGQLLAGTDESPGDTEIYKGRSFKVYRGMGSIS
                     AMKAGSSDRYFQEGAKKLVPEGIEGRVPYKGSVSDTVFQLVGGVKAGMGYVGAANLEE
                     LREKATFVRITNAGLRESHPHDVHITKEAPNYMIAGAEYEES"
     gene            complement(9173..10981)
                     /gene="aspS"
                     /locus_tag="PLM83_01455"
     CDS             complement(9173..10981)
                     /gene="aspS"
                     /locus_tag="PLM83_01455"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOK70107.1"
                     /translation="MSYVLRTHTCGELAAGNVGETVALCGWVQSVRDHGGLIFVDLRD
                     RYGRTQVVVSPDLSEDILERARSIHRESAIRVEGIVSERPEGTKNPSISTGDVEVMAS
                     NIEIIGDAAPVLPMEVSDENLANEEVRLRYRYIDLRRPSLQANMMARHRAAMAVRNYL
                     SAQGFLEIQTPLLVRSTPEGARDFVVPSRNFPGKFYALPQSPQLYKQLLMIAGFDRYF
                     QIAPCFRDEDQRADRQLVHSQIDIEMSFCDEEDVFSVVEGIISAAFKGAIGADVKGPF
                     LRMTYDEAMERFGSDKPDMRFGMELVDLTDLVADSEFGIFKSVCATGGRVRGISAPGC
                     AGFSRKQIDDLTELARIYRAKGLITLKVEGGEIQGSVAKHISSDTLGEIASRLGSADG
                     DLILIIADTPEVSATALGHLRRYLGKELGLIPKGDYKLLWVTDFPLFEWNPDDGRWDP
                     MHHIFTMPREGDLELLESDPASVKGRQYDLVLNGTELGSGSIRINDPAIQKRVMNVIG
                     VTPEEAEEKFGFLLRAYQYGGPIHGGFAIGFDRLLAVMLGLESLRDVIAFPQNAAGVS
                     LVDDCPNEIDPKQWKELHLKLEDCAGKPDAEARSDC"
     gene            complement(11105..12490)
                     /locus_tag="PLM83_01460"
     CDS             complement(11105..12490)
                     /locus_tag="PLM83_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOK70108.1"
                     /translation="MYSRPEAKPQPARALSIIFIFLALSTILVGAITDFVVTEVMSSR
                     LNRAFQAEVENAAVTWPEDLPTGLDYGSKSVPSDCKRDLAALQPDRPRRVRALYATGW
                     TAGSGSRMKQLVDLVCDTELNSIVIEIKDDLGVISYRSDVPLATEIGASTGKIADLRR
                     LLDELYDSGVYPIARLVVFKDPQLAAARPDLAVRDSSGVPWHDSQGLMWTDPRKKEVW
                     EYNLAIAREAARAGFREIQFDYVRFPDDAGLARTNWLDLEGKNRVDVISDFLDYAGRE
                     LEPYKVVITADIFGIVCSSRSDMGIGQKLEEMAKHVDYLAPMVYPSHYRKGEYGIANP
                     DAAPRETVKRSLSDAQDRIAKAGGRAKIIPWLQDFSLGYRYTAAEVRAQIDAAAECGI
                     YEFMLWNPRNLYTAGALGRPDPEDALRPRPHLDLLAGSGAIRLEDPVIVTEWRALSAL
                     KQAGVGQILGP"
     gene            12614..>12812
                     /locus_tag="PLM83_01465"
     CDS             12614..>12812
                     /locus_tag="PLM83_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70109.1"
                     /translation="MSTVLSCGLLQMDPAQFKTVHDFQVSIAARISSLARRGAELVVM
                     PEYAAMIGAGLETAPHAPGNMF"
BASE COUNT         2855 a         3812 c         3075 g         3070 t
ORIGIN      
        1 tatgcggttc tgctcaactt agcgcctccc gtgacgctcg actgccgcac ccggatcgga
       61 tgcggcagtt gtcgccaatt atgtccaact cggtcttcgc tagcggcccg tgttaaaagc
      121 ctccacttcg tagaactgaa gagcattcaa gggatttgat accctagcaa caacgaacct
      181 aaccttcgac acgctcatag ctgtaaactg aaactcgaat gacaccacgt ctccagaccg
      241 cttcatgtac ttctggtatt cagacggttt tgtaaatgac ccaactacaa tccagcgtcc
      301 gttctcttcg acctccacag tcatggaata cgggtagtta cctacatcac tgctcgttct
      361 tatggtattg atcacccgcg aggccgagaa ctcaacttcg agccattctg tcttgccgcc
      421 ggtaattggc ccggaagctt cccaatatgt cttcatattg ccatcaatag ccatagaagc
      481 aggtcttgcc ggtccatctg agctagccgg cgcatagctc gacgcgctag cccgccctcc
      541 attggccgca gctgcccagt tcacgttgac cgtcccgatt ggcggcggtg gcgccacaga
      601 ccccgtagta aacgagaacc ctgaagccac cgaaacgttt cctgcagcgt ccttcgatac
      661 tacctgatag tagtatgtcg tgttatcgcg aagaccagtc aatgtcacgc tatggctagt
      721 cttaagagac cccgaaggca caacaggagt gattattacc gatctagcgc ccacatctcc
      781 tgcagggctc gacgcctccg aactcgacac ggatttgtcg cctgcggcat ccttcatggt
      841 cgtgcccgtc aagccatatt caatggttga agtagcaggt tcatcggtgt tccatgttat
      901 cgtagccgaa tttgcagtaa tattcgccgt ccgtacgtta gttatcttcg gaggacgagt
      961 atccaaggtt atcgacgcag agtatgtcct gctctgattc ccatagatgt cgcgaaactc
     1021 gacatagact gtcttgttcc catcaccagg acttagcctg tatgtcatcg cctgttggaa
     1081 ctgcatccac ccggtccagt tcccgctgcc ctcacgcagg cgcatctcga ttggacccgg
     1141 cgagtcgtct tttgcagcta tcgagagcgt tacgtcataa tagcgcgtat actgagcccc
     1201 attgtttatc gcaatgctgc ctgttggcgg attcctgtcg ccaagctgag acgtacggaa
     1261 cgtcagcgtg gaggactccg ccatatttcc tgcggcatcg gcggaacgaa ccctgaagta
     1321 gtagtcagtg ttggggctta agccgtacag ggcgaccgaa tggctcgtca cgaagtcata
     1381 ctggcccata accaagttcg ggctgtatgt gccgtaatac acccaggaat cggagttctc
     1441 gtcagtgctc catgttataa cagcagaatc cggtccgatg ttctttgtct gcaccgacct
     1501 gattataggg ggctccgtat cgagggttat tacagcctgc gcttgggctt cgtttcccgc
     1561 tgcatctctg accttcacat agacggtcct cctgccatcg cccgtggtaa gctcccaggt
     1621 aaccctgtca gaatacgccc tccaggaacc ataagaggac ttatcatccc aaatcctcat
     1681 ctcaagcgga ccgcctgagt cgtcctgagc agtaatctcc aactgcacag ttctgctctt
     1741 tgtgtattgc gcgcccccgt ttatttgcac tctcacagtt ggaggtgtcg tatcgggagg
     1801 ctggatccta agactcatct ccgatgaccg cgcacggttg cccgctgcat cggtggaagt
     1861 cactgtgtag tagtatgtga caccagaacg caaattggac aatgtaaccg catggtaagt
     1921 cgaaagcacg ctcgtgccca cacggttctg gggcactccg tatgcccagt actccacgta
     1981 ggaatctgcc ggctcatcgg tggtccatgt gatgctcacc gagtcgagcc ccgttgcttt
     2041 tgcctctatc cggcttatca ccggcggagt tgtatctaca ggctgaatca caagcgtgag
     2101 atcctgcgat tgcccccggt ttcctgccgc atcggtagaa attaccctgt agtaatacgt
     2161 cgtgcccgca cggaggttcg aaagggttac cgagtgggat gtggcaagca tgctcgtgcc
     2221 cacacggttc tggggcactc cgtatgccca gtactccacg taggaatctg ccggctcatc
     2281 ggtggtccat gtgattatcg ccgaatccag ggggcccctg gttgcctgta ccccatatat
     2341 cctcggcggc tgtgtgtcta aagaaggagg cgcagtcatg acagcctcga tcgacgcatg
     2401 gttgcctgct ccgtcaacca cggaaaaccg gactctcggc gtcgcaccaa cacctgcagg
     2461 caaagatatg gcaaaatcac ttccatacca gtcgaaccag cgccaatcat tgccgtcata
     2521 agacatccaa aacccgacta tcccactagg atcattggcc gaaaggacaa ctctcgtcct
     2581 gccatcataa cctccgtaac cgcctgtttg catgctctcc aagcgtgcag tggctactgg
     2641 tggcgtcgag tcgagcacta tcgtccccga ttcgccgggt atgcctatac cgaataggcc
     2701 acgaaatttc acgtatactc tctttgtccc gtctcccgga gtcaattccc aagtgaactc
     2761 tccatatggc tcaggcttcg aaaaccagga gctccaggtg gaattgtcat tactaaaagc
     2821 gacagttgag taatcactgt aaactgcgag ccgaaccgtc cggctgtttg tatattcagc
     2881 gccatcgttt atcacgattc cgctgaacgg tttgtccggt ttgctccctt ccgccactat
     2941 ggacttgggc tgcacgccaa ggggcgatac accgtaaccg cccatttcac gttcgatgcg
     3001 ctccttgtaa atggcggcta tgcggttttc agcattgatt cccagggcat ggttgacagt
     3061 ggcaagcgcc gcatttgcat tgccggtctt ccactgggct atacccttta aaagcagcgc
     3121 attatcagat ccatacgttg gcaatgcgtc cacaagttcg ctgaacgtct gcaatgcttg
     3181 ggcatatgaa cccgattcga gcagagccga accaagtccg tatcttgccc gtaacagatt
     3241 cggattggcc gacgcggcag ctgaatagca ctgcacagcc tgtgccgact gccctgaaag
     3301 cctgtatatg ttgccaattg catcattagc cagtatcgac tcgggatccc tctgcaccgc
     3361 ctgacggtat gcatccaatg cttgcgtgtg gagtccgcgc gcagccatcg catcgccaag
     3421 ccgcttgtaa agataggctg gcgcgccaga cctgcttgta gcctgcaaca gcaactgctc
     3481 tgcctgatca tatctgccac tcctgtattc caacactgca gatacaccaa gaatcacggg
     3541 gttgagcctg tcgccagttg cagagataat acgatcaatc tgcgcagtcg ctgcctggat
     3601 attcccttcc tccaggtata tataggcaag gagaacagca gggtcgactg ccgtcctatc
     3661 ctgagcgacg gccctctcca aggcaacttt ggcatctccg ggtcttccgc taatgaaatg
     3721 gagaactccc tgtcggtaat ggccttctgc atacggcggc gagtagagtg ccgcctttga
     3781 gtaggcttga atcgctcgtt cgacatcttg atatccctgt tctgaaaggg gtgggaagcc
     3841 cagctttctg actgaatccg gttccgtaaa aagctccatc cgataattga acaccctggc
     3901 agccttcgac ctcatctcgt aggcgcaccc aagcccgaag tgagcgcggg tgtttgtcgg
     3961 ttctatgtat attgcctgtt caaactggcc tatagcttca tttgcagcac cctgtgcgag
     4021 atactgctcg ccccgcagaa tgtaatcata agctgaaatc tgcgcttgcg aactagttga
     4081 caccaatgtg cctacgaaaa gcaccagggc taagctagcg aagatgaata gcaccttcct
     4141 tttgctcttc attacctctg ccctccctat ttgttggact cagtaggtat agacgctgtg
     4201 cgaagccagc ggttccaata tgcaagaacc ccgcttgcct gttcgtcacg cggggtccac
     4261 catcgagcgc tccaatagat atttcatgaa ctacgggtat atgcgattga ttaggcgcgg
     4321 gaaaggaatc atctccctaa catgctcgct gccggtaatc cacgctactg ttctttcgat
     4381 ccctagcccg aacccggaat gggggaccga tccatagcgc cggagatcaa ggtaccaacc
     4441 gtaggcttcc tcagggagat tatgctcgcg catccgcgcc gccaggagat ccaggtcgtg
     4501 aattctctca ctgctgccga tgatctcgcc gtagccctca ggcgccagca agtcagagca
     4561 ttttactatc tcgggcctat ccggatcagg ctgcatatag aaggccttta cctttgttgg
     4621 ataaccggtt acaaataccg gcttgtcgta tttcatcgaa atcgtagcct cgtcgggcgc
     4681 cccgaaatcg tctccccact cgaattcctt accctccccg tgcaacagtt ccacggcctc
     4741 atcatacgaa agccgcggga atggcggcac aatacactcc agtttcgaaa ggtctctttc
     4801 gagcgtgatc agctcctgcc ggcgtttggc aaggcaacgc tgtacgatgt atgacaagaa
     4861 ctcttcctga atcaccatat tgtcttcctg agtggcatag gccatctcgg gctcgatcat
     4921 ccaaaactcg ataagatgcc ttcgcgtttt agatttctca gccctaaagg tcggaccgta
     4981 gcagtaaact ttgccaaatg ccatgcacgc cgcctcattg tacagctggc cgctctggct
     5041 cagatacgcc ttttcgccga agtagtcggt ctcgaataag gtagtagttc cttcgcatgc
     5101 cgccggcgtc agaattggcg cgtcgatctt gacgaacccg ttgcttcgca gccagtcttc
     5161 tgccgcagaa atcacctcgt cccgcaccag caacgcagcg tgttgtcgag gattacgaat
     5221 ccacaagtgc ctgtgatcaa gaaggaaatc cacgccgtgt tcctttaggg ttattgggta
     5281 ttcctcagcc agctgtacaa gctccacgcc tgtagctgac atctcgaatc cgcccgccgc
     5341 cctcttgtca gcgcgaatgc gcccggtgac caatatcgac gattcttgcg tcaggttgtt
     5401 gcacagttca aactggtcat cagggatttc gccacgaacg gcgacagctt gcatgaaacc
     5461 tgtcccatcc cttattatca ggaaatggat gcctccactc gaccgcttgt tatatagcca
     5521 ccccttaata gtgacctctt ctccatcatg ttctcgtatg tcggcgatac ggacggactg
     5581 caataaccac acctccggat gacctcatct tcatcaagat gtttccatag taactactat
     5641 tataattgcc cagccaacgt ttctcaacca cagcccaatt acggatcgga tctgtcctcc
     5701 ccgccggacg ggtcacatct gtacgcgacg tccaggtagg cgtatgcggc aaaggactga
     5761 gcagccgcga atatgtgacc gtgaagcgcc tgaccatttc cactatagag tatcagcctt
     5821 gccccagata atgcgcggtc tgcggtgacg catagaactc tctcctccct accccgatcc
     5881 caaaacagac gcgggcctct gctaaagaac atgaccgttt ttcttgggcc taccggcgca
     5941 atgtctttca tccctctgca ataagcatat gttaaaggac ccgaggccgt cgtctggcat
     6001 ggtactgccg cgtgcaccca tcaagcttgc tagcttatag tagtctttta catgttgcgt
     6061 gccgggccgg gcggcgcatt gaacagagct agggcggcaa catggtgcat gcggcgagac
     6121 actgctggat catggatcct actggcttca acactcccta cctcagatgc actgatcgac
     6181 tcggccctgc cacacgaaat gtggcaccac atccaaacat ccacatggag tttcggatat
     6241 gagcagggtc ggggagtcta aaccccgacc ctgctctgcc gtgctgctat atttttgggc
     6301 aaccgtctcg tgctaaagaa tcagttgcct atatgccaaa tctccaaacg caaccgtacg
     6361 tagtcgatgt attcaattcc aaagaccaga cccgtcgtga tgctgtaatc ctctacagtt
     6421 accctcaccc tgaacggata ctgaatcctt tcatataccc ttccgggcca gggcgcatca
     6481 ggcccactcg ggttgatctg catgaattcc actggcccca ctgcattcgc agtgatgctt
     6541 cctgtcactc tcgacagcat gccgaggatc gggtctgcgt tgatgaccac cttgcccgta
     6601 gaacctctaa aagtgaacgt ctcagtgaac ggcccggttt gaggccattt taccagcatg
     6661 ctcttgttta caagctgaac ctcgtcgccc caatcggatg acgtaggcag cttcacaagt
     6721 gtcgcatcta ttcggtatgg atttggcgag ttcaccgtga tctggcgcga atacgtatag
     6781 tagttcttct tcattatcgc aatgtcatgc acgccatagc tcacgcccgt aatcgtcagc
     6841 ggcgtggtcc cctggtctac cccgtccaga aaaacggttg cccccgacgg cttagaggta
     6901 atgctcagcg ttccgtattt caccgacggt ggtggcggcg gcggcgttac aacactcccc
     6961 actgtaaatg ttgcgaccgc cgacgaccaa tcttggccga agttgggggt aatcgaaatc
     7021 gacttcacct caaacatgaa cctgtctgcg gatatattga catttagctg tggcccgaat
     7081 gaaccctgct caagtgaacg ccttagccat tcaacgtcgc gtatctgcga tggcgtggcg
     7141 atcagtacga gctgctcaac tcctgcagga ccgccaatgg tcaggttata tctggagttg
     7201 tcaggcaagg agtatgtctg acctgcccgt ttgtagttgt attctgagta tatgttgggg
     7261 aatatcaggc tcacgttgcc gacggtatcg atgtcaaaaa tgtagatata cgcatctttg
     7321 ctcgtcctga agtagacatt cagcctttcc cccggattat actgcgactt gtcagtccaa
     7381 atgctggtct gatagggggg attcgggttg atgattatgc tcttcacatc atactccgcc
     7441 agagcagact cgctcgagat catggacaca accataacca taattaggct tacagccaaa
     7501 agactgcacc tcttgatctt ggtcgacacc gcaagtcgtc ctcctctcgg aaaaagtagc
     7561 ctacgattct tcgtactcgg ccccggcaat catgtaattt ggggcctctt tcgtaatatg
     7621 cacgtcatgt gggtggcttt ccctaaggcc tgcgttggtt atccgcacaa acgtggcctt
     7681 ctccctgagt tcctcaagat tcgcagcccc cacatatccc attcctgcct tcacaccgcc
     7741 cactagctga aaaacagtgt cagaaacaga tcccttgtag ggcacccgcc cctctattcc
     7801 ttccgggaca agcttctttg caccctcctg gaagtatcta tcgctcgatc cagccttcat
     7861 cgcgcttata gaacccattc ctcgataaac cttgaagctt ctccccttgt agatctctgt
     7921 atcgccagga ctctcatcgg tccctgcgag gagttgccca accatgacca catgcgctcc
     7981 cgcagccagt gctttagtca tgtctccgga gaacttgaca ccaccgtcag cgactatcct
     8041 gattccagct tccgcggctg cagcggcaca ctcgagaatc gccgagaatt gcgggactcc
     8101 gacccctgcc actacacgtg tagtgcagat ggagccgggc ccgataccca ctttgacaca
     8161 gttcgcgcct gcctctgcta atgctttgac gccgtcacca gtagcaacgt tccctgccat
     8221 gatgtcgata tctccgaaat tggctctgat agccttcacc gtctcgatga cgcccctaga
     8281 atgtccgtgg gctgtatcga caactatcat gtccgcgcca gcatcgatga gagcagcaac
     8341 cctatccatg gtgccaacag agacccccac ggcagcccct acgcgaagtc tcccgtgttc
     8401 atctttggct gcgttggggt attttcgcgt cttcttgatg tccttgatgg taatgagccc
     8461 tttcaacttg aactcctcgt caaccagtgg cagcttctcg actttgtgca ccttgagtat
     8521 ctcgatagcc tcatcgacag tagtccccac gggcgcggtg atcaaaccat ctttggtcat
     8581 aacgttggac accggctgat cataatcggt ctcaaatctc agatcccggt tggtaagtat
     8641 cccgacgagt gtaccgtctt cagttattgg gacgcccgag atatgataac gttccataat
     8701 ctcaagtgcc tctcttactg aggcttcagg cgaaaggaat atcggatcga caatgatgcc
     8761 gtgttctgag cgtttcacct tgtcaacctc gagggcctgc tcttcaatgg acatgttttt
     8821 gtgaattatg cccattccgc cttcacgggc aagggcgacg gccattcgag cctctgtcac
     8881 agtatccatg gccgccgaca tcagcggaat attgagcatg atcttatcag taatctcggt
     8941 cctaacatct acattggctg gaaggacttc tgagatgccc gggacaagca aaacgtcgtc
     9001 aaaggcgaga gcctccctga tcttgtctgg gttatgcatg tcgtaatcta cctccggagc
     9061 ataagtattt agtcaaggca accctgaatc agacatcaat ggattaatca aaagcccgtg
     9121 cgccccgttc cctggaacgc acggaccaaa cggcctcctc atgccgcacg catcagcagt
     9181 ccgaacgcgc ctcggcatcg ggcttgcccg cacaatcttc aagcttcaga tgaagctctt
     9241 tccattgttt cgggtctatc tcgttcgggc agtcgtccac cagagaaacg cctgcggcat
     9301 tctgaggaaa agcgatgaca tcgcgcagac tttcaagtcc aagcataaca gctaggagcc
     9361 tgtcgaaacc gatggcgaat ccgccatgga ttgggccgcc gtattggtac gcgcgaagca
     9421 ggaatccgaa cttctcctct gcctcctcgg gagtaacccc gatgacgttc atgaccctct
     9481 tctgtatagc gggatcgtta atcctgatgc ttccactccc gagctctgtg ccattgagaa
     9541 ccagatcata ctggcgaccc ttgacagaag cgggatctga ctcgagcaac tcgaggtctc
     9601 cctcccgcgg catggtgaat atatgatgca tcggatccca tcggccatca tctggattcc
     9661 attcaaatag cggaaaatct gtaacccaaa ggagtttgta atcacccttc gggataaggc
     9721 cgagctcctt accgagatat cgccgcaaat gccccagagc cgtggcagac acctcagggg
     9781 tatcggctat gatcagtatg aggtctccat cggcgctgcc cagcctcgat gcgatctcac
     9841 cgagggtgtc agaggatata tgcttggcaa cgcttccttg gatctcgccc ccttcgactt
     9901 tgagggtaat tagccccttc gcacgataga tccgagccag ttcagtaagg tcgtctatct
     9961 gcttgcgcga aaagcctgca caaccagggg cagagattcc tcggacacga ccccctgtgg
    10021 cacacacact cttgaatatg ccgaattccg aatccgccac gagatccgtg agatctacga
    10081 gctccatccc gaacctcatg tccggtttgt cagatccgaa cctctccatg gcctcgtcat
    10141 acgtcatcct gaggaatggt cctttgacgt ctgccccgat cgcccctttg aatgctgcgg
    10201 atatgatgcc ctcaactacc gaaaacacgt cttcttcatc gcaaaaactc atctctatat
    10261 cgatctgact gtgcactaac tgcctgtcag cccgctggtc ttcatcgcgg aaacacggag
    10321 cgatctggaa atagcgatca aatcccgcta tcatgagcaa ttgcttatac aactgagggc
    10381 tctgcggaag tgcatagaac ttaccaggga aattcctgct tggcactaca aagtcgcgcg
    10441 ctccctccgg agtgctccgc accaacagcg gtgtctgaat ttcaagaaag ccctgggccg
    10501 aaagatagtt ccggacagcc atggcagcgc ggtgacgcgc catcatgttt gcctgcagcg
    10561 agggacgcct caggtcaatg tatctgtatc tcaacctgac ctcttcgttg gccaagttct
    10621 catctgacac ttccatagga agcaccgggg cagcatcccc tataatctct atattactgg
    10681 ccataacctc gacatcaccg gtagatatcg aaggattctt tgtgccttcc ggcctttctg
    10741 agacaattcc ctccacccgg atcgcgcttt cgcggtgaat gctccttgcg cgctccaaaa
    10801 tatcttctga aagatcggga ctgacaacta cctgcgtacg accgtatctg tcccgaagat
    10861 caacgaagat caatcctccg tggtcgcgca ctgactgaac ccatccgcac agcgcaacag
    10921 tctcaccaac atttcctgct gcaagctcgc cacaagtatg agtgcgaagc acgtaactca
    10981 cctcaaaacc ctcaattact tatttccacc cgcatctaca gctaatacga atttaatcca
    11041 attatagacc tgcgcctgga ctcgtgcaac caaggccgcc ccatcgtaac ccagccggcc
    11101 tggcctaagg gcccaggatc tggccgacgc cggcctgttt cagtgccgac aatgcccgcc
    11161 attcagtgac tatcacggga tcctcaagac ggatagcacc gcttccggca agcaggtcca
    11221 aatgcggcct aggacgaagc gcatcctccg gatccggacg cccaagagca ccggctgtat
    11281 acaggttcct cgggttccag agcatgaact cgtatatgcc gcactcggcg gctgcatcaa
    11341 tctgtgccct aacttcagcg gccgtatacc gataccctag ggaaaaatcc tgcagccatg
    11401 gtattatctt tgcccggccg cccgcctttg caatcctgtc ttgtgcgtcg ctaaggctcc
    11461 gttttacagt ctccctcggc gccgcatcgg gattcgcaat gccgtactcg cccttgcggt
    11521 aatgcgacgg atacaccatt ggcgccaaat agtctacgtg ctttgccatc tcctccagct
    11581 tttgcccgat gcccatgtct gatcgagaag agcagactat cccgaaaatg tccgcggtga
    11641 tcactacttt gtatggttca agttcccgcc cagcataatc aaggaagtcg gaaatcacat
    11701 cgactctgtt tttgccctca aggtctagcc aattggtgcg ggccaatcca gcatcatctg
    11761 ggaacctcac gtagtcgaat tgtatttcgc ggaagccggc tcgcgccgcc tcccgggcta
    11821 tcgcgagatt gtactcccac acctctttct tcctaggatc cgtccacatc aagccttgac
    11881 tgtcgtgcca gggcacgccc gaagaatccc gcacagcaag atcgggacgg gcggccgcaa
    11941 gttgcgggtc cttgaacacg accaggcgag ctatgggata aaccccgcta tcgtaaagct
    12001 cgtcaagcag gcgccgaaga tctgcgatct tgcctgtaga cgctccgatc tctgtcgcaa
    12061 gcggcacgtc cgatctatag cttatcactc caaggtcgtc ctttatctct atgacaatgg
    12121 aattcagctc cgtgtcgcac acaagatcga caagctgctt catccgcgac ccgcttccag
    12181 ccgtccagcc agtagcataa agagccctaa cgcgcctagg cctgtcgggc tgcagcgctg
    12241 caaggtcacg cttgcaatct gaaggcacgg acttgcttcc ataatcaaga cccgtcggaa
    12301 gatcttccgg ccatgttacg gcagcatttt ctacttcggc ctgaaaagcc cggttcaacc
    12361 ttgatgacat gacttctgtg acgacaaagt cagtgatggc accgactagt attgtcgaga
    12421 gtgccaagaa tatgaagatt atggacagcg cacgcgctgg ttgaggtttc gcctccggac
    12481 gtgaatacat atacagtgta tactctccct tccacccacc cgagttttca ttctactcac
    12541 agctttccgt tccttctcgt cggtgcagga acagtgtgga taatgtgtaa tacttcttct
    12601 gggggtgacc tgcatgtcga ctgttctttc ctgcggattg ctgcaaatgg atccagccca
    12661 gttcaaaacg gttcacgatt ttcaagtatc gattgccgca aggatatcat ctctggccag
    12721 acgcggagcc gagcttgtag taatgcctga atacgccgca atgataggcg ccgggcttga
    12781 aactgccccg cacgcccccg ggaacatgtt cg
//
LOCUS       DAOSAI010000018        12689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3329_length_12689_cov_3.958287, whole genome shotgun sequence.
ACCESSION   DAOSAI010000018 DAOSAI010000000
VERSION     DAOSAI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12689
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3329_length_12689_cov_3.958287"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..681)
                     /locus_tag="PLM83_01470"
                     /pseudo
     CDS             complement(1..681)
                     /locus_tag="PLM83_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903157.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1188..1676
                     /locus_tag="PLM83_01475"
     CDS             1188..1676
                     /locus_tag="PLM83_01475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOK70110.1"
                     /translation="MGGNAVEEAFKMMTYGVYIIGTAAAGKINGMTAAWVTRVSVDPP
                     LLLVAISKGHYTTELIRASRVFSVNLVSPQQAELAAKCGSVSGRICNKLVDEEIEYGE
                     TGAPILTEAAAWLDCEEIDEFECGDHICFVGRVVGAGKRSEQALMYRKGSMQPVPAQN
                     DA"
     gene            complement(1804..2487)
                     /locus_tag="PLM83_01480"
     CDS             complement(1804..2487)
                     /locus_tag="PLM83_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70111.1"
                     /translation="MASITAADVVSSIKAIAGTIDFASLGPYLMLNEDYLRHYLSAIL
                     NWDFRLLNLTGAIQPVQLHPEWPTYKEQTHLDYGRYGRLTNGEGETEYWPKEHGSAGF
                     IDFAIGDYESPEIGIELTMEYGWTHEEIVYDFIKLMDARNPFKVAISYNVLLRPAGFV
                     DRVDEPQHLLDNMNRAIADASARLGDGICDDSRQLVFIFTETDEDARRRHWHYDQHLQ
                     TFVKGLPNT"
     gene            complement(2658..3242)
                     /locus_tag="PLM83_01485"
     CDS             complement(2658..3242)
                     /locus_tag="PLM83_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70112.1"
                     /translation="MRKRIVTLLLFAVGLSLLTAGCPDTGIFQKKPAASYSKEDTFGP
                     YRITVSSVAKAKDTTAPEEGKITVARTGMKTVSTVFETDPVYTSWVTDLDSNDKFEVL
                     VWSVSPDEEKRGALHVYEWNEPELREQPFPDLTEAQKAGYRGHDDYSVDNNKVVRSFP
                     VFSTEGGADAQSSHKRALWYSYHNGEWKLEQESE"
     gene            complement(3800..5044)
                     /gene="larC"
                     /locus_tag="PLM83_01490"
     CDS             complement(3800..5044)
                     /gene="larC"
                     /locus_tag="PLM83_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOK70113.1"
                     /translation="MMIAYFDCYSGISGDMILGALIDAGLDPDDLLSELSRLEQGGLE
                     HFDLAVSKVLRKGIGGTHVEVITAEAHQKRDREHSHDHHHGHDHDTLFRNLSAIEKLL
                     DGSGLDAEVRETSRAIFRRLAEAEALVHGIPVDRVHFHEVGAIDTVVDVVGAVAGLRI
                     MGVERVFASPIHVGTGLVRCAHGTLPVPAPATAELLRNVPVYATGVRGELATPTGAAI
                     ITTLAESFGPIPEISIERIGYGAGRHDFEISNLLRVLIGKTMQHYSTEDGDAACDLVT
                     VECNIDDMNPEFYEHVMDLLLKRGALDAFVTPVLMKKGRPGHLITVLTPRAALDIVTT
                     TLFEETTTLGVRIQEVSRRTAQREWINVSTEYGVVRVKVARFGDRLVNVAPEYEDCRK
                     LAKEYGVPLKAVYDEVKALARE"
     gene            complement(5055..5828)
                     /gene="larB"
                     /locus_tag="PLM83_01495"
     CDS             complement(5055..5828)
                     /gene="larB"
                     /locus_tag="PLM83_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOK70114.1"
                     /translation="MDPKSILLLLESVRDGDIDPGEALEELRMLPYEDLGFAKVDHHR
                     NLRTGFPEVIYSSGKTPEQVARIAQAILDRGASVFLATRADTDTFDAVRQVAPDAVYH
                     ETAHIISVQRDLPTPTEGMVLVAAAGTSDMPVAEEAAVTAEIMGNKVERLYDVGVAGI
                     HRLLDKVDLLRSARVLIVVAGMEGALASIIAGLVDKPVIGVPTSVGYGASFGGLAPLL
                     TMLNSCAAGVSVVNIDNGFGAGYLASIINRLGEDNPDPI"
     gene            complement(5863..6777)
                     /gene="larE"
                     /locus_tag="PLM83_01500"
     CDS             complement(5863..6777)
                     /gene="larE"
                     /locus_tag="PLM83_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOK70115.1"
                     /translation="MPEHAPKGHCPGNCVSHPSHRDYSPHCSSDSGNALEAKLDNLES
                     ILAEMGSALVAFSGGADSAFLLATAHMVLRGKVVAATISSPVHSRTELDQARRFADGL
                     GIEHEVIHLDLLGIPRFASNPSDRCHTCKRAVFEALLSLAQQRGIPYVIDGSNADDAS
                     DYRPGMAALTELGIESPLRGAGLTKQEVRTLSRRMHLETANKPASPCLATRIPYGHTI
                     TQAKLDQVQLAEDFIRTLLPKDSDHMQLRVRHHGDTARVEVTPELMHCIVDHAISISE
                     FFRQLGFTYVALDLGGYSQGNLNRLLEP"
     gene            complement(6888..7220)
                     /locus_tag="PLM83_01505"
     CDS             complement(6888..7220)
                     /locus_tag="PLM83_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOK70116.1"
                     /translation="MRVSEEYDLIVTIINKGWADEVVEASRRAGAEGGTILYGRGTGV
                     HEQKTFFGILIEPEKEVVLTLIPRSRTDAVMEAVVAAGQLEKPGTGIAFVLDASKVTG
                     IVHRKDAE"
     gene            complement(7187..7957)
                     /locus_tag="PLM83_01510"
     CDS             complement(7187..7957)
                     /locus_tag="PLM83_01510"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOK70117.1"
                     /translation="MGEIIVFQGFPAVMAEVGMTLIPLAILFFIFQTFFLKLPRTFMR
                     KVVVGMISTFIGLSIFLQGVNIAFLPIGKMLGAKIGGLSNPIYALPVGFILGLLVVLA
                     EPSARVLSGEVEKASAGSIRESVLLRTMASGVAVLVGLGYYFIARGIALTWIIIPGYI
                     IALILLKWVDPAFAAIAFDSGAVATGPLTVTFLMSLGLGIAAEKLGGPTMNAGFGLVC
                     LIALASTLAVEGMSFIFKRNSKKEDGRKCACQKNTTSS"
     gene            complement(7957..8649)
                     /locus_tag="PLM83_01515"
     CDS             complement(7957..8649)
                     /locus_tag="PLM83_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOK70118.1"
                     /translation="MSTPLQEAIAEVVAAVAPLTIAVAVLSIIFLGFSWTNLIQLLIG
                     AFFTTVGLFLFLRGTRIALLPMGEMIGAYLPRHGSMALLVATSFIFTFSVTIADPSVH
                     VLVSQVASFPDAGINSDFLAIFISAGMGLLVTVAILRIVLNVPIKYIFLGAYSTAIIL
                     SFFTKPELVPFFFDSGSVSTGPMAVPCIISLGLGTASVLQRKQSLADGFGLVGLATLG
                     PILVLMIWGVFI"
     gene            complement(8808..9434)
                     /locus_tag="PLM83_01520"
     CDS             complement(8808..9434)
                     /locus_tag="PLM83_01520"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOK70119.1"
                     /translation="MDPKWLKWGLRLQAVAQNGLSYAHGPYDIERYELIRSIATEMMS
                     EQAHADPEIVRDIYTLETGYATPKIDVRGAIFADSSILLVKEKADGCWTLPGGWAEIG
                     ESPSEAVEREVFEESGYRVRASRLLALYDRGKHPHPPAPYHIYKLFFECEIIEEPSTP
                     SPDGIETDAVEFFPSDRIPNLSIPRVTPDEIRRLFQLHADPQAPTDFD"
     gene            9710..>12689
                     /locus_tag="PLM83_01525"
     CDS             9710..>12689
                     /locus_tag="PLM83_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70120.1"
                     /translation="MRNPDFHHAMSRRYKMLVFDWDGTAVTSRRGPVDHLIWRTEALL
                     GRGVWLAAVTGTNFVNLNRQFFSLLNPKVKTNLLACVNRGAEVYGFDSEGRPVRLWAR
                     EATCAENTMMDQAVTMAQRVLREQYGLATRIISDRMNRRKLDIMPFPEWEDPPKERIG
                     ELLDETQGRLRIAGLEGIESVIDLVGRCSEEVGLDGRITSDVKHVELGLTDKADSVDY
                     LVEEVAKTHGIEVSEILFIGDEFGSIGGCTGSDSKMITPQTESASFISVGQEPEGVPP
                     AVCHMGGGIESFAKILEAQIEMWRDDEENMFGRDRLTDSLLLLPAEDNWVAYETCCDA
                     ETAASYETLFALTNGYLSVRGAHEDGLYRGNAGSFVAGVFDKSSGPYDVTELVVIQDP
                     LPVEIVVDGERVGNEGVRDYRRELDMRGGLLRRFYRWSDGAGRVVRVESERFVSSVDE
                     HLLCARLSVTMERGSGRVLLRSRLDGNVSNSGAVHLKVDSVEARGDGTAEVCTVTMES
                     QAGIAMACAQQASFVEAPGKARGEPDEALGTPVYPRPFSGESAAGHDFEVRLAKGDSI
                     EICKFVAVHTSRDVAWRACKAPPAMDQLRTAAQASVEQAASRGFSGCRLEHLEEWRRQ
                     WERCDIRVDGPDRDQMAVRFAVYHLAAMGPQSDDRVSIAAKGIHGEGYKGHVFWDTEI
                     FILPFYVAQFPDVARRLLMYRYHTLPGARRKAREKGYRGAMYAWESADSGDETTPKWS
                     APHPGTGERVRIWCGELEDHITADVAYAVWNYCRWTGDRGFMKEHGMEMIVEAARFWA
                     SRAQWNEEKSRYEILGVIGPDEFHEHVNNNAFTNYMARWTLLAAFRLAEDFPIKWEAL
                     LRRLGISEAELDEMRRVTNLLYLPLPDPVSGVIEQSEGYHGLEDVDLAELKAIAEDSG
                     KSVEENFGRERFIGSKLIKQADVVMLGRLLPGLFGEDIWKANWDYYEPRTTHLSSLSA
                     SVHSTFASHLGLPEEAYAYFE"
BASE COUNT         2769 a         3296 c         3702 g         2922 t
ORIGIN      
        1 ctggctgaaa tacctgcgca atacgatcca ctcgtcgttc tgctccatca ttactacatc
       61 ggcgcgacgt ttgatctcct tgttcagcct ctcaagcggg ttggtcgagt gaatctgtct
      121 gtgatgctct ttcgggaacg ccatgtacgc gagaatttca tcttcacagg cacatagcat
      181 ctgcgcaact ttgagaaacc tttcttccag ggtggctgca acttgccgca gttgggcctt
      241 ggcagtcagc tgatcaggtt gggcgaatat cgtccgaata taggcggcga ccatttgcta
      301 ggctccttgc ggcaaagcat ttcgcatgaa atgcactctc ccatcagagc cttctgtatt
      361 gcattcttaa gaccttcatg ggcgttgtac tccgcttagc ccccgtgata taagatcctt
      421 gaggaactcg taccagaatg gctcaccctt atagcaaagc ccaggatctc gcgttcgcca
      481 ggattcgact ctgtcgatgc catctgggcc caaagctctt acaaggtcgt ctaccttgcg
      541 tgtgctgatg ccgtgaacat aagcttcctg aactactgat aggatagctt tctcggtcat
      601 cttgcgaggc tctagaaaac tcggaaagaa acttccttct ctgagcttgg gtatcctcag
      661 atcgagattg ccaactcgtg ttcccctccg attactttcg cagcttcaag ttcaatgagc
      721 ctttgggtca gaagctgcgc gccttccctc aataaatcct ggtgtagaat gtccaaaagt
      781 gacatgtctc tcttgacagg tgccatctcc cgaatttaca ccactacttg agactctaac
      841 ggctagccca gagcattcct cgtcagcgag gggcagagta tctatggttt tgtggtgtgg
      901 ttctggtctt tgcaggatta tcctttttgc ttgttgtgag gacgtcttga cgtcattcct
      961 ggcggctcta ccacagtcaa gcaatcggca tgcacgacac ttgagatcag tttaccccgg
     1021 aggtatcgct gggtggcaaa gagagatgct gcgatcatgg ttactttgat gtttgtgtgc
     1081 taacgatgct acgctgtgca aagtaagcta gaaggttaat gaagctcgcg tctagaatta
     1141 agcgtctagc agcatccgct ggggcggata gctaggagga gagaatggtg ggcgggaacg
     1201 ctgttgaaga agcgtttaag atgatgactt atggtgtgta cataataggc acggcggcag
     1261 cggggaagat caatggcatg acggctgcat gggtcacgcg agtatcggtc gatcccccat
     1321 tgctgctcgt agcaatttca aagggccatt acactacaga gctgatcaga gcatcgaggg
     1381 ttttctcagt aaacttggtc agccctcagc aggccgaact tgccgcaaag tgtggttcag
     1441 tctctggccg gatatgcaac aagcttgttg acgaagagat agagtacggt gaaacgggcg
     1501 ctccgatact gaccgaagct gccgcgtggc tcgattgcga ggaaattgat gagttcgaat
     1561 gcggagacca catatgcttc gtgggcagag ttgtcggcgc cggcaagaga tcggagcagg
     1621 cgctgatgta caggaaaggg agtatgcagc cggttcctgc ccaaaacgat gcttgaggaa
     1681 agattgatcg gggaataggc cttaagaaca ggtatgagcc ggaagggccg atggtccgcc
     1741 gcggtcaggc gacgggatac ggtcacttcc ggctttccgt tgtgttgtcg accgatccgc
     1801 tttctacgta ttcggcagac ccttaacgaa ggtctgcagg tgttgatcgt aatgccaatg
     1861 gcgccttcgg gcatcttcgt ctgtttcggt gaatatgaat acgagttgtc gtgaatcgtc
     1921 gcaaattccg tctccgagcc ttgctgatgc atcagcgatc gctctgttca tgttgtcgag
     1981 cagatgctga ggttcgtcca ccctgtccac gaaaccggcg ggacgtagga gcacgttgta
     2041 ggagatcgca accttaaacg ggtttctcgc gtccatgagc ttgatgaagt cgtacacgat
     2101 ttcttcatgg gtccacccat actccatcgt cagctctatc cctatctcgg gactctcgta
     2161 gtctcctatc gcgaaatcaa tgaaccctgc tgatccatgt tccttcggcc agtattctgt
     2221 ttcgccttcg ccatttgtca gccgcccgta ccggccatag tccaagtgtg tctgttcctt
     2281 gtatgtgggc cattcaggat ggagttgcac tggttgaata gcccctgtca ggttgagcaa
     2341 tctgaaatcc caattgagta tcgcagaaag gtaatggcga agatagtcct cattgagcat
     2401 gagatacggc ccgagcgacg cgaagtcgat ggttcccgct attgccttga tgctgctgac
     2461 tacgtcggcc gcggttatcg atgccattca tatcgccccc tggcagtagt ctaaaactgc
     2521 tatgcatgga tgggcggtgg cgatgggcat gcatgagcct gaacgcaacc gccatgttcc
     2581 tgcttcgagt tcttgttggt tcttcgccat cgagttccat ataccttcgt ccgccagggt
     2641 gagcagaccg cgcgatctta ctctgattcc tgttcgagct tccactctcc gttatggtag
     2701 ctgtaccaca aagcccgctt gtgcgaagat tgggcatctg ctcccccttc ggtcgagaaa
     2761 acggggaagg atctgacgac cttgttgttg tcaactgaat agtcgtcgtg ccctctgtac
     2821 ccagccttct gtgcttcggt cagatcgggg aatggctgct ctctcaattc gggctcgttc
     2881 cattcgtaca cgtgcaaggc cccgcgcttc tcttcatccg gggacacact ccataccagc
     2941 acttcgaact tgtcattgga gtcgagatcc gtaacccacg atgtataaac agggtcagtc
     3001 tcgaagaccg tcgagacggt cttcattcca gtgcgtgcca cagttatctt gccttcttca
     3061 ggagcagtcg tatcctttgc cttggcgact gaggacacag taatcctgta cgggccaaat
     3121 gtatcctcct tggaataact cgcagcaggc tttttctgaa aaatgcctgt atcggggcag
     3181 cctgcagtaa gaaggctaag acctacagcg aatagtagaa gcgtcacaat ccgttttctc
     3241 atttctgttc ctcccttatg catttggaat agtgcactcg cgggcacagc tattattccc
     3301 aaaagtggca cttgcataac cacagtgaag aaattgtgag ggagggggag ggaagccatg
     3361 ggatcaggat gagctggcgg ttgattggga gcatgaagct gacgttacgt gtgtgggttt
     3421 tgccatgatg tcatcttggt cgaaacccga tcgtccctcg ggcagcagaa ccatgtttga
     3481 actctgaagc tggtgccgct tcgccgagct ccagactatt tctgaaagaa gattgccatg
     3541 agttcgcatt tgggtttcac cttgcacttg gggtctgttt atgcaccggg tattgccata
     3601 aggaggcaga ctccgttttg atgctgtggg acatggctaa aacttgaaca cttgcaattg
     3661 aaaaatatgt caaagctaga catgccccca aacgtttacc ccggagcccg gcagagagaa
     3721 gctggctata tgatgatggc gctctggtcg caggtttgcg cggagaagca cctgcacaga
     3781 gataacagac gaggactatc tattcgcgcg ccagcgcctt gacttcatca tacaccgcct
     3841 tcagcggaac cccgtactcc ttagcaagct tcctgcaatc ctcatactcc ggcgcgacat
     3901 tgacgagtct atcgccgaat cgcgccactt tcacccgtac gactccatac tccgtagaga
     3961 cattgatcca ttcgcgctgg gccgtccgcc gtgagacttc ttggatgcgc acgcccaggg
     4021 tcgtagtctc ctcgaaaagc gtagttgtca caatatccag tgcagcgcgg ggagtgagca
     4081 ccgtaatgag atgtccgggg cgtcctttct tcatcagcac gggggttacg aacgcgtcaa
     4141 gggcgcccct tttcagcaga agatccatca cgtgctcgta gaactccggg ttcatgtcat
     4201 cgatgttgca ttccacagtg accaggtcgc atgctgcgtc tccatcctca gtggaataat
     4261 gctgcatcgt cttgccgatg agcacccgaa gcaggttgga aatctcaaaa tcgtgccgcc
     4321 cggccccgta tcctattcgc tctatgctta tctcaggtat gggcccgaag gattccgcga
     4381 gggtagtgat gattgccgcg ccggttggcg tggcaagctc tcctcgaacg ccggtggcgt
     4441 acaccggaac gttgcggagc agctcggcgg tggcaggcgc cggcacgggc agggtgccgt
     4501 gggcgcacct cacgaggcct gtccctacat ggatgggcga tgcgaatacc ctctccactc
     4561 ccataattcg caggcctgca acagcgccta ccacgtcaac aaccgtatct atcgcgccta
     4621 cctcgtggaa atgcacgcgg tcgacgggaa tcccgtgaac taatgcttcg gcttctgcaa
     4681 ggcgccggaa gatggcgcga ctcgtttcgc gaacctccgc atccaggccg ctcccatcga
     4741 gcagcttttc tatggccgag aggtttctga ataaagtgtc gtgatcatgg ccgtgatgat
     4801 ggtcatgaga atgctcgcgg tcgcgctttt ggtgggcttc ggccgttatg acctcgacgt
     4861 gtgtgccgcc gatgcctttc cgcagcactt tgctgactgc gaggtcgaag tgttcgagcc
     4921 cgccctgctc gagccgggaa agctctgaga ggagatcatc cgggtcaagc cctgcatcga
     4981 tgagggcgcc caggatcatg tcgccgctga tgccgctata acagtcgaaa taggctatca
     5041 tcagtgaatg cctcctaaat ggggtcggga ttatcctcgc ccaaccggtt gattatcgac
     5101 gcgaggtatc ctgcgccgaa accgttgtcg atgttgacca ctgacactcc ggcagcgcag
     5161 ctgttcaaca tggtcagaag gggagccagt ccgccgaaac tggcgccata gcccacgctg
     5221 gtggggactc cgatgactgg cttgtctacc aggccggcaa ttatgctggc gagagcgccc
     5281 tccattcctg ccacaactat gagcacccgt gctgaccgga gcagatcgac tttatcgagc
     5341 aggcggtgaa tgcctgccac accgacgtca tacaggcgtt ccaccttgtt acccatgatc
     5401 tctgcagtta ccgccgcctc ttccgcaaca ggcatgtcgg aagtccccgc ggccgctacg
     5461 agcaccatac cttcggtggg ggtcggcaga tcccgctgga cggatatgat gtgcgctgtt
     5521 tcatggtaga ctgcgtcagg tgctacttgg cggacggcat cgaaggtgtc tgtgtctgcg
     5581 cgagttgcca agaatactga ggctccccga tccaagatgg cctgggctat ccgcgctacc
     5641 tgctcaggtg ttttgccact cgagtatatt acctccggga acccggttcg aagattgcgg
     5701 tggtgatcga ccttggcgaa accgaggtct tcgtatggca acatgcggag ctcctccagg
     5761 gcttcgccgg ggtctatgtc gccgtccctt accgattcga ggagaagcaa tatgctcttg
     5821 ggatccatcg tgtgcatcgc ccctttcaac gcttcgccat agtcagggct cgagtagccg
     5881 gttgaggttg ccctgggaat agccgcccag atcgagggcg acgtatgtga aacccagctg
     5941 tctgaagaat tccgagatgg atatggcgtg atccacgatg cagtgcatga gttcgggtgt
     6001 gacctccacc cgcgccgtgt cgccatggtg gcgcacgcga agctgcatat ggtcagaatc
     6061 tttgggcaat agcgtccgga tgaaatcttc ggccaactga acctggtcga gcttggcctg
     6121 ggtgatcgtg tgaccgtagg gtatccgtgt agccaagcac gggctggctg gcttgttcgc
     6181 ggtctcaaga tgcatccttc gggagagtgt gcggacttcc tgcttagtca accctgcgcc
     6241 cctcagcgga ctttcgatcc ccagctctgt aagggccgcc atgccggggc ggtagtccga
     6301 tgcatcatca gcattggatc cgtcaatcac atagggtatc ccgcgttgtt gggccaggga
     6361 gagcagggct tcaaagacgg cgcgcttgca cgtatggcat cggtcggaag ggttgctcgc
     6421 aaacctggga atgcctagaa gatcgaggtg gatgacttcg tgttcaatgc cgagcccgtc
     6481 tgcaaatcgc cgagcttgat ccagttctgt tctgctgtga acaggggagg atatcgttgc
     6541 ggcaaccact tttcctctaa gcaccatgtg ggcagttgcg agcaggaacg cgctgtcggc
     6601 cccgccggag aaggccacga gcgcgctccc catctcagcg agtatgcttt ctaagttgtc
     6661 cagcttggcc tcgagggcat tgccagaatc agagctgcaa tgggggctgt agtcgcgatg
     6721 cgagggatgg gaaacgcaat tgcccgggca gtgccctttc ggcgcatgtt caggcatctg
     6781 tcagtgcctc ctttccaggt tggggcttgg ggccccgtaa accccctcgc agggaggatc
     6841 gttgccgttc agcctgacag atagatcatg tagggcgccg gccggtgcta ttctgcatcc
     6901 ttcctgtgga cgattccagt gactttgctg gcatccagca cgaatgcaat tccagtcccc
     6961 ggtttttcca gttgtccggc agcgacgact gcttccatca ctgcatcagt gcggctacgc
     7021 ggaatgaggg tcaaaaccac ctctttttcg ggctcaatca gaatcccgaa gaaggtcttc
     7081 tgttcatgca ccccggtgcc ccgtccgtag aggatagtgc cgccttcggc gcctgcgcgc
     7141 cgagacgcct caacaacttc gtccgcccaa cctttattga ttatcgttac gatgaggtcg
     7201 tattcttctg acacgcgcat ttcctcccgt cttctttttt cgagttcctc ttaaagatga
     7261 agctcatgcc ctccacggcg agcgtggaag cgagagcgat caagcatacg agaccaaagc
     7321 ctgcattcat ggtgggccct cccagttttt cggcggcgat tcccaggccg agcgacataa
     7381 ggaaggtgac tgtcaacggt cctgtggcca cggcgcctga gtcaaaggcg atagcggcaa
     7441 aggccggatc aacccatttg agcagaatga gagctataat gtaaccgggt atgattatcc
     7501 atgtcagtgc gatgccgcgg gcgataaaat agtagcccag tcccaccagc acagcaactc
     7561 cgctggccat tgtgcggaga aggaccgatt cgcgtataga ccctgccgag gccttttcca
     7621 cttcaccgct gagcacccgg gctgacggtt ccgcgagaac caccagaagc cctaagatga
     7681 atcccacggg aagagcgtaa ataggattgg acagcccgcc tattttcgcg cccagcattt
     7741 ttccgatagg aaggaatgct atgttcactc cctgcaggaa aatggagagc ccgatgaacg
     7801 tcgatatcat tcccacgaca actttgcgca tgaacgtgcg cggcagcttc aaaaagaagg
     7861 tttggaatat gaagaataga attgccaggg gaatcagggt catgcctact tccgccatga
     7921 ctgcggggaa gccctgaaag acgatgatct cgcccatcag atgaacaccc cccagatcat
     7981 gaggaccagg attggcccta aagtagccaa accgacgagt ccgaatccgt ccgccagcga
     8041 ttgcttgcgc tgaagaactg atgcggtgcc cagtcccagc gatatgatgc atggtactgc
     8101 catcgggcca gtcgatacgc tgcccgagtc aaagaagaac ggtaccaatt cgggtttggt
     8161 gaagaaggac agaattatcg ctgtagaata ggcccctagg aaaatgtatt ttataggaac
     8221 gttcagaact atccggagta tggcgacagt taccagcagg cccatacctg cggagatgaa
     8281 aatcgccaga aagtcggaat tgatgcccgc gtccgggaag gacgcaacct gcgacacaag
     8341 cacgtgcacc gaaggatctg caatggtgac actgaatgta aagataaagg acgtggccac
     8401 cagcagcgcc attgagccat ggcgaggcag gtaggcgccg atcatctcgc ccattggcaa
     8461 cagcgcgatt cgtgtgcccc gcaggaacag aaaaagccct actgtagtga aaaatgcgcc
     8521 gataaggagc tgtataaggt tagtccatga gaagcccaag aagatgatgc tcagtacggc
     8581 cactgcgata gtgagaggcg ctactgctgc tacaacttct gcgatggctt cttggagcgg
     8641 agtagacact gggcaatctc ctttccgagt gtaatgagtt ttgttagatg ggctactccc
     8701 ctattatcgc atatatgaga gcaaggggga aattcggagc tgttcgcttg ctcgaggatg
     8761 tcttggggtt ccacccgcca gggaggcaac gttctgatgc aacggcctca atcgaaatca
     8821 gtgggcgctt gggggtcagc gtgaagctga aaaaggcggc ggatttcgtc tggagtcacg
     8881 cgcggtatgg agagattggg aatgcgatct gatggaaaga actccactgc atcagtctct
     8941 atgccatcgg gggaaggcgt agagggctcc tcgattattt cacattcgaa aaagagcttg
     9001 tagatgtgat aaggcgctgg cggatgcggg tgcttccccc gatcatataa ggccagaagc
     9061 ctcgatgccc gtactctgta acctgattcc tcgaatactt cgcgttccac ggcttcgctc
     9121 ggagactcgc ctatttctgc ccatcctcca ggaagagtcc agcacccatc ggccttttct
     9181 ttcactagga gaattgagct atctgcgaat atggctcccc gcacatcgat tttcggggtg
     9241 gcatacccgg tttccaatgt gtagatatcc cggactatct cgggatcggc atgggcttgc
     9301 tcactcatca tctctgtggc gatggatctg ataagctcgt atctttcgat atcgtaaggg
     9361 ccatgtgcat aagagagccc gttctgtgcg accgcctgaa gtcgaaggcc ccatttcagc
     9421 cacttcgggt ccatgattcg ctcccccttc ccttaacttg caagttctcc ggattgcccg
     9481 agactcctgc cggcatgtct tcccaagtca tctgcaactg cctgaaacat cgccgagtct
     9541 gctatggggg atgggcagga aacgcagtag ggcgggagga ctcatcgctt aaacgttaaa
     9601 ggaatctgac cgaataatgg aggatatgcg tattgcgatg acgaatatct ctcctgtaaa
     9661 gcctgcatac aatgtaactt ttacgattaa gtagatgtag ggggtcccaa tgagaaatcc
     9721 tgactttcat catgcgatgt cacgccggta caagatgctc gttttcgatt gggacggaac
     9781 agcggtcacc tcccggcggg gccctgtcga tcatctcata tggagaacag aggccttgct
     9841 tggcaggggc gtgtggttgg ctgccgtcac aggaacaaat ttcgtcaacc ttaacaggca
     9901 gttcttttcc cttcttaatc cgaaggtgaa gacgaatctg ctggcgtgcg tgaacagagg
     9961 agcagaggta tacggctttg attctgaggg acgccctgtt aggctgtggg cccgggaggc
    10021 aacgtgtgcg gagaatacaa tgatggacca ggcggtcacc atggcgcagc gtgttttgag
    10081 agagcagtac ggccttgcaa cacggattat ctccgacagg atgaacaggc gcaagcttga
    10141 cataatgccc tttcccgaat gggaagaccc tccgaaggag cggattgggg agcttctcga
    10201 tgaaacccag gggcgcttga ggattgcagg ccttgaggga atcgagtcgg tgatagacct
    10261 ggtgggccgt tgctctgaag aggttgggct cgatggcagg ataacgagtg atgtgaaaca
    10321 tgtcgagctt gggctcacag acaaggctga ttccgtagat tacctggtcg aagaggtcgc
    10381 aaagacgcat ggcatagagg tgtcggagat tcttttcata ggagacgaat tcggatcaat
    10441 cggcggatgc accggcagcg attcgaagat gattaccccc caaacagaat cggcgagttt
    10501 catcagcgtg ggacaggagc cggagggcgt tccgcctgcc gtctgtcaca tgggaggcgg
    10561 gatcgagtcg ttcgcgaaga tactcgaggc ccagatcgag atgtggaggg acgatgagga
    10621 gaacatgttt ggtcgagata gactcacaga cagcctgctg cttctgcctg cagaggacaa
    10681 ttgggtcgct tacgagacat gctgcgatgc cgagactgct gcatcgtatg agacgctgtt
    10741 tgcattgacc aacggttatc taagtgtgag gggtgcccat gaagacggtc tgtatcgggg
    10801 taacgcggga tcgtttgtcg caggggtctt cgacaagtct tcgggaccgt acgatgtgac
    10861 ggaacttgtc gtgattcagg atcctctgcc cgtggagatt gtagtggacg gcgagagggt
    10921 cgggaatgaa ggggtcaggg attaccgccg ggaactggat atgcgcggcg ggctcctgcg
    10981 ccgtttttac agatggagcg atggcgctgg acgggtagtg agggtagaga gtgaacgctt
    11041 tgtatcctcc gtggatgagc acttgctctg tgcgaggctc tccgtgacca tggagcgggg
    11101 gtcggggagg gtattgctcc gatcccgcct ggacggaaat gtatccaatt caggcgcggt
    11161 tcacctgaag gtagattcag ttgaggcccg gggcgatgga acggcggagg tatgcacggt
    11221 gactatggag tcgcaagcag gcatagccat ggcttgcgcc cagcaagcaa gcttcgttga
    11281 ggctccgggc aaagcccgtg gtgagcccga tgaggcgctt ggaacccccg tttatccccg
    11341 gcctttctca ggtgagtccg ccgctggcca cgattttgag gttcggctag caaaaggtga
    11401 ttccatcgaa atttgcaagt ttgtagctgt gcatacctca cgggatgtgg cgtggcgtgc
    11461 ctgcaaagcg ccgcctgcga tggatcagtt gaggacggca gcgcaggcga gtgtggagca
    11521 ggctgcatcc cgcgggtttt cggggtgcag actagagcat ctggaggaat ggcgcagaca
    11581 gtgggaacgc tgtgatatcc gtgtggacgg accggatcgg gaccagatgg ctgtgaggtt
    11641 tgccgtgtat cacctggcgg cgatggggcc tcagagcgat gacagggtga gcattgccgc
    11701 caaagggatc catggcgaag gctacaaggg ccacgtcttc tgggatacag agatcttcat
    11761 tcttccgttc tatgtagccc aatttcccga tgtggcccgc cggcttctaa tgtacaggta
    11821 tcacacactg cccggggccc ggcgaaaagc tcgggagaaa gggtatcgag gggccatgta
    11881 cgcctgggaa agcgcagatt ccggcgatga gactaccccg aagtggtcag ccccacatcc
    11941 ggggacaggg gagagagttc ggatttggtg cggagaactg gaggaccata tcaccgcgga
    12001 tgtggcatat gcggtatgga attactgtcg atggaccggg gatcggggtt tcatgaagga
    12061 gcacgggatg gagatgattg tggaggccgc tcggttctgg gcgagccgcg cccagtggaa
    12121 tgaggagaaa agcagatacg agatactagg tgtgatcggc ccggacgaat tccatgagca
    12181 cgttaacaac aacgccttca ccaattacat ggctagatgg acattgcttg cagctttccg
    12241 gcttgccgag gattttccga taaagtggga agcgctactt cgccgactcg gcattagcga
    12301 ggccgaactg gacgagatga ggagagtgac caaccttctt taccttccat tgccggatcc
    12361 ggtttcagga gtgatagagc agtctgaagg atatcatggt cttgaggacg tggacttagc
    12421 ggaactgaag gcaattgctg aggattctgg aaagtccgtg gaagagaact ttgggcgcga
    12481 aaggtttata gggagcaagc tgatcaaaca ggctgatgtg gtaatgctgg gtcgcctctt
    12541 gccgggcctg ttcggtgagg atatttggaa ggcaaactgg gactactacg aaccgcgaac
    12601 cactcacctg tcgtcgctca gcgcgtccgt ccattcgacg tttgcgtcgc atctgggctt
    12661 gcctgaggag gcatatgcat acttcgagt
//
LOCUS       DAOSAI010000019        12609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3360_length_12609_cov_3.774016, whole genome shotgun sequence.
ACCESSION   DAOSAI010000019 DAOSAI010000000
VERSION     DAOSAI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12609
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3360_length_12609_cov_3.774016"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLM83_01530"
     CDS             complement(<1..206)
                     /locus_tag="PLM83_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70121.1"
                     /translation="MGDVSPVGMDLGTAGTMMRLSLPYGLAAGAGDRRNLAKSVELET
                     AGGSDAAAEAGARARENERLAQACS"
     gene            complement(228..1391)
                     /locus_tag="PLM83_01535"
     CDS             complement(228..1391)
                     /locus_tag="PLM83_01535"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOK70122.1"
                     /translation="MRRRRSGLFKIAAWLALLLMSCSIPRVYGAQPTEVVESQASQGE
                     GSSQPALTGSAGKDGATVRVKDITRVYGVRDNQLIGYGLVVGLKGTGDSRNTPFTART
                     LSNMLDKFKITVDPLEIKAKNVAAVMVTGVLPAFASPGDAIDVAVSSVGDARSLAGGT
                     LLLTELAGADGRVYTVAQGPVVTGSEAKATVGRVPAGATVERHVPMEMLTPDGHLVLS
                     LLNPDYTTAARIARAVCDRFGQGAAVAVDGGTVRVSVPEWLQETPTPFIAEVGELEVA
                     PDAPAKVVLNARTGVVVIGGNVKVSAAAVSYNGFNVSVGGLPGWQDGAGASSGNVVAM
                     PAQPGSTITLEAGSTIEALAAALNAVGARPTDIMNIMEALRACGALQAELQIM"
     gene            complement(1409..2083)
                     /locus_tag="PLM83_01540"
     CDS             complement(1409..2083)
                     /locus_tag="PLM83_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOK70123.1"
                     /translation="MVKIAKAFGRTRGMVMEDRSGAAAAMAVAAAVAFAAVLVFSGGV
                     VRATSLYSEDASPFASMYADRKARQVGDLVTILVAEVSYAQNRSQTNTAQGIGVTAQP
                     GVGVFDFIPAARLDVGDSSKGANETTRSGRLVGTMTAEVIEVLPNGDLVIRGSREVSV
                     NREKEIMVVEGVVRPQDIGPNNTIPSTMVANAQISMSGGVYTGQKGGFFKTLWDGLVW
                     VWNTIF"
     gene            complement(2077..3036)
                     /locus_tag="PLM83_01545"
     CDS             complement(2077..3036)
                     /locus_tag="PLM83_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HOK70124.1"
                     /translation="MKCAIPKIKEPCRRLAGKRPAVAAAIVVAVVSASVFTSGGVAMA
                     SANEAVDDVHWLSGPDLTFGPEGTVPTPSDYADMTIELLPSACSEGPQILLGEIAVLH
                     VEDDALREALRSLEVGSAPLPGSSRKVNLGQVQVRMRARKIDPAKFVFVGAPEVAVAA
                     AARRIAASDIEAAAIDAVCVFTGHALSPSDFRVARTDAPQGMLVPPGELRLEAQVAQT
                     GADMAFVRVRVRAIVDGVEARSVLVWLEITAPSAVKQLADVTLMAQTGDVRVSVPAVA
                     LQDGRPGQKIRVKNAVTGVVVWARVMDSQTVTVCAADMEGSPW"
     gene            complement(3059..3847)
                     /gene="flgG"
                     /locus_tag="PLM83_01550"
     CDS             complement(3059..3847)
                     /gene="flgG"
                     /locus_tag="PLM83_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOK70125.1"
                     /translation="MMRALWSAATGMHAKQLDMDVIANNLANVNSAGYKKSRVDFQDL
                     MYQTIRSAGSTVAQGSRVPTGIQVGLGTRAAAIQKVFSQGDFVQTENPLDMVIEGDGF
                     FQVLMPDGSIAYTRTGAFKLDADGRIVTSDGYVMEPEIVVPDNAVSISIGTDGTVSVE
                     IAGENEPQELGSIELARFLNPGGLTSMGRNLYRPTAASGEPIIGTPGLDGMGTVAQQF
                     LEMSNVKIVEEMVNMIIAQRAYEVNSRAIQASDDMLATATNLRR"
     gene            complement(3998..4729)
                     /gene="flgF"
                     /locus_tag="PLM83_01555"
     CDS             complement(3998..4729)
                     /gene="flgF"
                     /locus_tag="PLM83_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HOK70126.1"
                     /translation="MLRGIYSAACGMRAQETRQSLIANNLANVATPGFKRDVGVGESF
                     SEMVVRSGRTPVGRLGMTSAITSAYFDMSEGSVSNTDCPYDFAIEGDGFFAVETDWGI
                     RYTRAGNFKLDADSYLVTMEGHRVLGDDGPIRVDGQLVAGSDGVLYADGREIGRLLVV
                     MPSSSDALTKEGGGLFAARGEVIPAGTGEVRVIQGALELSNVSVVREMVDMIAGYRVY
                     EAAQRALLSHDACLDRAINEVGRVG"
     gene            complement(4958..5419)
                     /locus_tag="PLM83_01560"
     CDS             complement(4958..5419)
                     /locus_tag="PLM83_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOK70127.1"
                     /translation="MKAEFINPFASAVMMVFQKEFGMNVMREQLDLHSKGPLTGSDVN
                     TIIGVTGQLEGQVIYTFEEGVAIRIASALVGETVEELDEMAKSAVAELTNIMTGNAAI
                     SLSENGYSCVLTPPTLLTGKELIVTTFTPILTIPFMTDFGTVTVHVALREK"
     gene            complement(5474..6304)
                     /locus_tag="PLM83_01565"
     CDS             complement(5474..6304)
                     /locus_tag="PLM83_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70128.1"
                     /translation="MGCGDGDVVRALVPILGDRTRDVRVITDLSTKKSRAFLLCLPED
                     GSARECVMVAKIYRDEGRALREAEVLAALESAGVAVPAACGATPTVLLRRYVYGPVLR
                     DVHWTLELARKLGAWLYSCHEALRLAPQGVGGYGRTRLVGDMNLGNFVIDVAQADGDE
                     AVAWRDCGTRGIVCGIDFGDTRVGDPLEDVGEGFMRILSHRPGYTMERQACAAAFVWE
                     YGRLSGAEDMVWSEVERHVLAAFRRVAAWRRDPFMNDVAEAVGAMWGSSTPQIHCDGV
                     "
     gene            complement(6308..6988)
                     /gene="mobB"
                     /locus_tag="PLM83_01570"
     CDS             complement(6308..6988)
                     /gene="mobB"
                     /locus_tag="PLM83_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOK70129.1"
                     /translation="MRVIAVSGLHRTGKTTTVEHIVGELVRRGYAVGTVKTIHREGFS
                     VDTPGKNTHRHRRAGAKIAAALSPGETAVIIDGKLSFEDLFSHYDTDWIIIEGAHEAP
                     FPKIVCARTEHDIEERVDEHTFLICGEVASAISSWRGIPVLDATSRIGEVVDMVESRV
                     PEGADRKMIDPAAYHGFKTAVYVDGKEIPMVPFVQDFVARTILGMLSSLRGYETGSEV
                     RVEIRRGE"
     gene            complement(7298..9157)
                     /locus_tag="PLM83_01575"
     CDS             complement(7298..9157)
                     /locus_tag="PLM83_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOK70130.1"
                     /translation="MFKHTKAKLLVTFLLVVVFVLIGNVMMYLQLANYDRNMTQVVDE
                     ELLSLQLVESLRAYDLEAANAISGLIIDPGNAGFQRDYDVAWEKTENAMRAAARRLTR
                     PELVEALSEVDELGKSMSTVAVSVTGMAGLSPEMALSIFSDTYEPVRRQRAEILDGLV
                     RAERDHIGRQIASSKASVKRGTLVASLVSAIGIAAAVAMALRVSRAVSEPVERLTHAV
                     NKVAEGDLTVQSDVDSRDELGHLAQAFNKTVESLREIVGKALDVSRQVAAASVQLSAS
                     VEQSADSVRQIADTAQQISAGAEQQSRSAGETANAAEALMPTVERVAVCARGQTNSVG
                     RGSAAVDEMRSSIAQSLEGLKRVSSAAQGVGEAAREGSTATRDMAISVNLARRESARV
                     SEAARDLDSSSKEIRRIVEVINDIADQTNLLALNAAIEAARAGEHGRGFAVVADEIRK
                     LAERSLAETKAIAKLVEDLGRSTEKVAEAIGGSDSATAEVSESVDRTMEVLNRIILGV
                     QESVELIEALSETAGSLEGSSDRVRRAMAEIVAASDSSLKAAEDMNVGIGQVKESIDA
                     VAAVSFENVAAVQEVTASTEEVDAAIREMHASSQALADIAAGLEELISGFKLT"
     gene            complement(9252..11111)
                     /locus_tag="PLM83_01580"
     CDS             complement(9252..11111)
                     /locus_tag="PLM83_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOK70131.1"
                     /translation="MFSKIRGKLLASFLAVAVVMGIATGLLYFGLERYEVGVRRLATE
                     EIMGLKTAESLRTADLMLSNAVCGIISDPYEPIFAAAYEDALTKMETATREATSRFKS
                     GREKEIAAGIAAGNEERERVERKIIDCASFDRIQAAAIFTEEYNPVRVRREQVLDEFV
                     SLQERKIEEAVEKAEAASDALQMAATVLTGASAALAVILAMAASRKIAVPIVQLTADV
                     KRVAEGDLTVETRVKSRDEIGDLADAFNRTVGSLSVLVSDAVSSSEIVASASLQLSSS
                     MQESAEAMQQISDTVQQMATAALQGSDSAEQSAAAAEQLSMAIGQVAAGAESQTTSVH
                     EASVMLGDMGTALAKSANGLENVSSATESTKEAAAEGSAAVDSMVTSVESARVEAARV
                     SDAARELDESSREIGRIVEVISDIADQTNLLALNAAIEAARAGEHGRGFAVVADEVRK
                     LAERSLAETKAIAGLVEGLRDSTGRVVTAIEVSNAATAEAAENASGATGLLEKILANA
                     NETATYVQELAAYGNDLITASARLQKAVEEIVMVADGNAAAAEEMEAGVGEVKASIDS
                     VAAVSQENAAAVEEVSAAASEVRASIESVAESADALAKMARNLREAVSKFKVG"
     gene            complement(11422..12282)
                     /locus_tag="PLM83_01585"
     CDS             complement(11422..12282)
                     /locus_tag="PLM83_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOK70132.1"
                     /translation="MRIAIVTDSIADLPADVASENGIFVVPLHVIFGEKSFLDGVDLS
                     SREFFARLKASDAAPSTSQPSPASFISLYDRLLREYDYIFSIHASSKLSGTFQSACIA
                     RDTLERGSIEVIDTHQATMCQGFVALAAARAVTAGAGPDGARDAIYRGISSARLFFTV
                     DTLEHLARHGRIGKAASMLGTMMSVKPIVSIIDGEVTPVAKAYGRRSILPRVIEFMGE
                     KIGPAQPIHAAIIDADSADRADEWRVKIESLFSCREIIMTECGPVVGTHAGPGAIGVA
                     WLPCGGADLE"
     gene            complement(12236..>12609)
                     /locus_tag="PLM83_01590"
     CDS             complement(12236..>12609)
                     /locus_tag="PLM83_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70133.1"
                     /translation="VFQGGVAANIGMRRAFERALGYEVVVPEHFDVMGAIGAAMLARR
                     HIESSKRSTLFAGWEVPEQTYTTSSRECSGCPNMCEIVEVARNSAVVARWGGRCGKWE
                     DMRRADHENCDRDRQHSGSPC"
BASE COUNT         2718 a         4351 c         2893 g         2647 t
ORIGIN      
        1 gaacaggcct gcgcgagcct ctcgttttcc cgggctctag cgccagcctc agctgcggca
       61 tcagaccctc cggccgtctc gagctcgaca ctcttcgcca agttcctgcg atcgccggcc
      121 cccgcagcaa ggccgtacgg gagcgagaga cgcatcatag ttccagccgt ccccaaatcc
      181 atgccaaccg gcgaaacatc gcccatcgct gcctgcacct cccacgctca cattatctgc
      241 agttcagcct ggagagcgcc gcacgccctc aaagcttcca ttatattcat gatatccgtc
      301 ggcctcgccc ctacggcatt gagcgcggca gccaacgctt caatggtact tccagcctca
      361 agcgtgatcg tgctgcctgg ctgtgcaggc atggccacca cgtttccgct tgacgcaccc
      421 gccccgtcct gccatccggg taagcctcct acactcacat tgaacccgtt gtatgagaca
      481 gctgcggcgc tcaccttcac gttgccgcct attaccacca cgcctgtgcg cgcattcaat
      541 acaaccttgg caggagcatc aggcgccacc tcgagctcgc caacttcggc gatgaacggc
      601 gtcggagtct cctgaagcca ttctggaaca ctcaccctga ccgttcctcc atcaactgcg
      661 acagccgctc cctgcccgaa cctatcgcat actgctcgtg ctatcctggc tgcagtagtg
      721 taatccgggt tgagaagcga cagcaccaga tggccatcgg gagtgagcat ttccatggga
      781 acatgtcttt cgactgtagc tcctgcaggc accctgccca cagtcgcctt ggcctcggag
      841 ccagtcacaa ccggaccctg agccacagta tatacccgtc catcggcacc ggccaattcc
      901 gtaaggagca gcgtcccgcc tgcaaggctc ctggcatctc ctaccgatga cactgcgaca
      961 tctatggcgt cacccgggct tgcaaaagcg ggcaaaactc cagtgaccat taccgccgcg
     1021 acattcttcg ccttgatctc cagagggtcg acggtgatct tgaacttgtc caacatgttc
     1081 gatagcgttc tcgcggtgaa cggcgtgttc cgggagtctc cggtaccttt gagccctacc
     1141 acaagtccgt accctatgag ttggttatct ctgaccccgt acactctggt aatatccttt
     1201 accctaacag ttgcgccatc tttccccgcc gagccagtca gcgcaggttg actactccct
     1261 tcaccctggc tagcctgaga ctccacaacc tccgtcggct gcgcgccgta gaccctgggg
     1321 atagaacatg acataagcag caacgcgagc cacgccgcaa tcttgaacag gcctgatctt
     1381 cgtcttcgca aaaccctcac ccccaaggtc agaaaatcgt attccacacc caaaccaggc
     1441 catcccagag cgtcttaaag aatccgccct tctgcccagt gtacaccccg ccgctcatgc
     1501 ttatctgcgc gttcgccacc atagtcgaag gaatagtatt gttcggccct atatcctgtg
     1561 gcctgacaac gccttcaacg accatgatct ccttctcgcg gttgactgag acctccctcg
     1621 aaccacgtat tactaggtcc ccattgggaa gaacctcaat aacctccgca gtcatggtcc
     1681 caaccagcct gcccgaacgc gtagtctcat tcgctccctt tgatgaatca cccacatcca
     1741 acctagcggc agggataaag tcgaataccc ctacacctgg ttgagcagtg accccgatgc
     1801 cctgtgccgt attggtctgg ctcctgttct gcgcatacga gacttcagca acgagtatgg
     1861 tcaccaagtc ccccacctgc ctggcctttc gatccgcata catcgacgca aaaggagacg
     1921 cgtcttcact gtaaagagac gtagcccgaa cgacgcctcc tgaaaagaca agcaccgccg
     1981 cgaaggccac cgccgccgca accgccatgg ctgcagcggc tccgcttcga tcctccatca
     2041 ccatgcccct ggtcctaccg aaagccttcg ctatcttcac cacggcgatc cctccatatc
     2101 tgcggcgcac accgtaacag tctgcgaatc cataacgcgc gcccaaacaa caactccggt
     2161 gacagcattc ttaacacgta ttttctgccc gggacggcca tcctgcagtg ccaccgcggg
     2221 cacactaacc cgcacatccc cggtctgggc catcaaagtc acatccgcaa gctgcttgac
     2281 cgcggaaggc gcagttatct ccaaccaaac caacacgctc ctagcctcca cgccgtcgac
     2341 tatcgcccgc actctcaccc tcacaaaagc catatccgcg cctgtctgcg ccacctgcgc
     2401 ctcaagccgc aactcgcccg gcgggacaag catcccttga ggcgcatcgg tcctggccac
     2461 cctgaaatca gagggagaca gagcatgtcc agtgaaaacg catacagcgt cgatcgcagc
     2521 agcctctatg tcggacgcag caatccgcct cgccgccgcc gcaaccgcaa cttcaggcgc
     2581 cccgacaaag acgaactttg cagggtctat cttccgcgcc ctcatcctca cctgcacctg
     2641 cccgagattg accttgcggg atgacccagg aaggggtgca gaacctacct ccagcgatcg
     2701 aagggcttcc cggagcgcgt catcttcgac gtgcaacaca gcgatctcgc ctaggaggat
     2761 ctgcggacct tcactgcagg cagaaggcaa cagctcgata gtcatgtcag catagtcgga
     2821 cggcgtcggc accgtcccct caggtccaaa cgtcagatca ggcccagaaa gccaatgcac
     2881 atcgtcaacc gcctcatttg cagacgccat cgcgactcca ccggaagtga aaaccgacgc
     2941 cgataccaca gccacgacga tcgcagcagc aaccgccggt cgcttcccag ccagacgccg
     3001 gcacggctcc ttgatctttg ggattgcgca cttcatttca gatcagcccc gcccgtggct
     3061 aacgcctgag attcgtggcc gtggcgagca tgtcatcaga agcctggata gccctggagt
     3121 tcacttcgta cgccctttga gcaatgatca tattgaccat ctcttcgact atcttgacat
     3181 tggacatctc caggaattgc tgtgcaaccg tgcccatgcc gtcaaggcca ggcgtaccaa
     3241 taataggctc gccagaagcg gcagtaggcc tgtacaggtt gcgccccatc gacgtcaggc
     3301 ctccgggatt gagaaaacga gccagttcga tgctgcctag ctcctgaggc tcattctccc
     3361 ctgcgatctc caccgacaca gtcccgtcag ttccaatcga gatgctcacc gcattgtcgg
     3421 gaacgacgat ttccggctcc atcacatacc catcggacgt gacaatcctt ccgtcggcat
     3481 cgagcttgaa tgcgcccgtg cgcgtgtagg cgattgaccc gtcaggcatg agcacctgga
     3541 agaagccgtc cccttcaatc accatgtcaa gggggttttc cgtctgaacg aagtcgcctt
     3601 gtgaaaagac tttttgaatc gcagcagccc tggttcccag tcccacttgg atgcctgtgg
     3661 gcacccttga cccttgagca acagtgctgc ccgcgctcct gatggtctga tacatcaggt
     3721 cttggaagtc aaccctgctc ttcttgtatc ccgcgctgtt cacgttggca aggttgtttg
     3781 cgataacatc catgtcaagc tgcttggcat gcattccggt ggccgcactc caaagcgccc
     3841 tcatcatggc aattcactcc ttcttttgtg tgggctgcct ccgtcattca gcagcccgcg
     3901 gcccccgctg cgcggtccgg ccgcatagat tagatctcga ttttcgtcca aagcacgtcc
     3961 ctcgccgccc cgggccgcca tcacccaatg cggcagacta acctaccctt cccacctcgt
     4021 taatggccct gtcaagacat gcgtcgtggg aaagcaatgc ccgttgcgcg gcctcgtaaa
     4081 cccgataacc ggctatcatg tcgaccatct cacgcacaac actgacattc gaaagctcca
     4141 aagccccctg gatcacccga acctctccgg ttcctgctgg aatcacctct cccctggcgg
     4201 caaagagtcc cccgccttcc ttcgtcagcg catccgagga cgaaggcatc accaccaaaa
     4261 gcctgccgat ctcccgaccg tccgcgtaaa ggacgccgtc cgaacctgca actagctgcc
     4321 catcaacacg gatcggacca tcatctccta agaccctgtg accttccatc gtaaccaagt
     4381 acgaatcggc atcgagcttg aaattgccag cccgggtgta cctgattccc cagtcggtct
     4441 ccacagcgaa aaacccgtcg ccctcaatgg caaaatcgta gggacagtcc gtattggaga
     4501 ccgagccctc cgacatgtca aaataggcag aagttatagc gcttgtcatc ccaaggcgcc
     4561 ccaccggcgt ccttcccgac ctcacaacca tctccgagaa agactcccca accccgacat
     4621 cccttttgaa gccgggggtc gccacattag ccaagttgtt ggcgatcaag gactgcctcg
     4681 tttcctgagc tctcatgcca caagccgcag aatagatgcc cctcagcaaa aacatccctc
     4741 ccttcccaga tcccgcgtca agcgtctcag agagccggca cggaacctcc gatatactga
     4801 agtaggcatt atcgccctac aacatacgta agcaagatgc atgccacagg aagtcaacca
     4861 gccatagacc agctatatgc ggcaaacagg cgctcccctc aggggagcgc ctctcatctt
     4921 ggtcatgcat ttatttctcg aactgtgcaa taattgctca cttttccctg agcgccacgt
     4981 gcacagtgac agtgccgaag tcagtcatga atgggatagt aaggattgga gtgaaagtag
     5041 tcactatcaa ttccttgccg gtcagcaagg taggaggggt cagcacacag ctgtacccgt
     5101 tttccgaaag agagatagcc gcatttccag tcatgatatt ggtcagttca gccacggcgc
     5161 ttttcgccat ctcgtccaac tcttctacgg tctcccccac caaggcagaa gctatgcgaa
     5221 tagcaacccc ttcctcgaag gtgtatatga cctgcccctc caactggcca gtgacaccga
     5281 tgatcgtgtt cacatccgag cccgtaagag ggcctttcga atggagatca agctgttcac
     5341 gcatgacatt catgccgaat tccttttgaa agaccatcat caccgccgat gcaaaaggat
     5401 ttataaattc cgctttcata cgccgccccc cgttccgttc actattactt atcgaccaaa
     5461 ggcgccaaag actttatacc ccatcacaat gaatctgggg ggtgcttgat ccccacatgg
     5521 ccccaacggc ctctgctaca tcgttcataa atggatccct gcgccatgcc gccactctcc
     5581 ggaaagccgc gaggacgtgg cgctcaacct cagaccacac catatcctcc gccccggaaa
     5641 gccttccata ctcccacacg aacgcggccg cgcacgcctg cctctccatg gtgtagcccg
     5701 gcctgtggct caaaatccgc atgaacccct cacccacatc ctccagcgga tcccccacgc
     5761 gcgtatctcc gaaatcgatc ccgcacacaa tcccccgcgt gccgcaatca cgccaggcaa
     5821 ccgcttcatc cccgtccgcc tgcgccacat cgatgacgaa attgccaaga ttcatgtccc
     5881 ccacaagtcg cgtgcggccg tagccgccga cgccttgcgg ggccaatcgt aaagcctcgt
     5941 ggcacgagta aagccatgcc ccaagttttc tggcaagttc caaagtccag tgtacatcgc
     6001 gcagaacggg gccgtaaacg tatcttctca aaaggacagt aggcgtagcc ccgcaggccg
     6061 cagggacagc cacacctgcc gactccaacg ccgcaagaac ttcggcctct ctaagggcac
     6121 gcccctcatc cctatatatc ttcgccacca tcacacattc gcgcgccgaa ccgtcctcag
     6181 ggaggcagag gagaaaagcc ctgcttttct tggtggacag gtcggtaatg actcgtacat
     6241 cccgggtcct gtcgccaaga atcggcacca gtgccctgac gacatcgcca tcgccgcatc
     6301 ccatagtcta ctcgccccgc cttatctcca ccctcacttc actcccggtc tcataacccc
     6361 tgagcgatga gagcatgccg aggatagtcc gggcgacgaa atcctgaaca aacgggacca
     6421 tgggaatctc ttttccgtca acgtagaccg cggtcttgaa accatggtac gccgcaggat
     6481 caatcatctt cctgtctgca ccttccggca cccgagattc gaccatgtca acaacctccc
     6541 cgatcctgga agtcgcgtcg agcacgggaa tcccccgcca gctcgaaatc gcggaggcaa
     6601 cctcgccgca tatgaggaaa gtatgttcat cgacacgttc ttctatgtcg tgctcagtgc
     6661 gggcgcacac tatcttcgga aatggcgcct catgcgcccc ttcgataatg atccagtccg
     6721 tgtcgtagtg actgaaaaga tcctcgaaac tgagcttgcc gtcgatgatg acggcagtct
     6781 cccccggact tagcgccgcc gcaattttcg cacccgcccg tctgtgcctg tgtgtgttct
     6841 tgcccggagt gtcgacggaa aacccctccc ggtggatggt cttgacagtg cccaccgcat
     6901 agccccgcct gacaagctcg cccacaatat gctccacagt cgtagttttc cccgtccgat
     6961 gaagccctga gacggcaata acccgcatag aatgaccccc atatgccacc ctgatgagcg
     7021 ttgccggccc atcgcattcc gcatggatat gtattctata gccacccacg ctttccctac
     7081 aggcccatgc acatcccagg gtttgcggtt gtcaaacctg gccagagtct ttgcgtcctt
     7141 tccacaagtt ctccaaccgg cacccgcaac tgccgcccca cccgtacatg cccgcatcaa
     7201 cagaaagccc accgcaacac gcatcacggg cgatgcacgg tagcggcagg cttttggcca
     7261 tcagactaca tgccatgtgg gcggggagga gacatcgcta tgttagcttg aacccggaga
     7321 tcaactcctc caaccccgcc gcaatgtccg ctagcgcctg ggaagacgca tgcatctccc
     7381 tgattgccgc atcgacctcc tcagtcgacg ccgtgacctc ctgcaccgcc gccacgtttt
     7441 caaaggatac cgcggcaaca gcatcaatag attccttcac ctgtcctatg ccgacattca
     7501 tatcctccgc agccttcaga ctactgtccg atgctgccac gatctccgcc atggccctcc
     7561 gcacccggtc actcgaacct tcgagagagc ctgcagtctc cgacaacgcc tcgatgagct
     7621 caacactctc ctgtacgcct aagataatcc gattgagaac ctccatggtc cgatctacgc
     7681 tctcggacac ctccgcagtg gctgaatcgg acccgccgat cgcctcggcc accttttctg
     7741 tggatcttcc cagatcctcc acgagcttgg caatggcttt ggtttcagcc aaagatctct
     7801 ccgccagttt ccggatctcg tccgcaacca cagcaaaccc gcgtccatgc tctcctgccc
     7861 ttgcggcctc tatggcggca ttcagcgcca ggagattagt ctggtccgca atatcattta
     7921 ttacctccac aatccttcta atctccttgg aactcgaatc gagatcccgt gccgcctccg
     7981 acacccttgc agattcacga cgggcgaggt ttacactaat cgccatgtcc cgcgtggcag
     8041 tgctgccttc cctggctgcc tcgcccacac cttgcgcagc ggagctcacc cgttttaggc
     8101 cctccaaaga ttgtgcgatc gatgaacgca tctcatcgac cgcagcagag ccccgaccca
     8161 cactattcgt ctgcccgcga gcacacaccg caacccgctc cacagtgggc atgagagcct
     8221 cagcagcatt tgcagtctcc ccagcgctcc tgctctgctg ttctgcgcct gccgatatct
     8281 gctgggccgt gtcggcaatc tgccgcaccg aatccgcaga ctgttccact gatgcagaca
     8341 gctgcacgct tgccgcagcc acctgccgcg aaacgtccag cgccttgcct acaatctccc
     8401 gtaaactttc caccgtcttg ttgaaagcct gcgccaaatg gcccagttca tcccgggaat
     8461 cgacatcgga ctgaaccgtc aggtctccct ccgccacctt gttgacagca tgcgtaaggc
     8521 gctccacagg ttccgacaca gccctcgaca ccctgagcgc catggcaacc gcggcagcga
     8581 tccctatcgc cgaaacgaga ctcgcaacaa gtgtccctcg cttcacgctg gccttggaac
     8641 tagctatctg tcgccctatg tggtcccgtt ccgcgcgcac caatccatca aggatctcag
     8701 ccctttgccg ccttaccggc tcgtacgtgt ctgaaaatat cgacagtgcc atctcggggc
     8761 tgagccccgc catgcccgtt acggatactg ccaccgtcga catggacttg ccaagctcgt
     8821 ccacttccga caacgcctcg accagctccg gccttgtaag acgcctggcg gcagcgcgca
     8881 tagcattctc cgtcttctcc cacgccacat cataatcccg ctggaatccg gcattgccag
     8941 gatcgattat caatccgctt atggcattcg ccgcctcaag atcatacgcc ctgagcgatt
     9001 cgactagctg aagcgataat aactcctcgt ctacgacttg tgtcatgttc ctgtcgtaat
     9061 tcgcaagctg cagatacatc atgacattcc caatgaggac aaagacaacc acaagcaaaa
     9121 acgtcaccaa aagcttagcc ttggtatgct tgaacaagcg cgaccctcct cagttggcgc
     9181 catgttcatc cccttgccga acatggacat gaccgaagat atagactcat gctaaatgaa
     9241 agaagaacct atcaccccac cttgaactta gatacggctt cgcgcagatt gcgagccatc
     9301 ttcgcaagag cgtccgccga ctctgccacg ctttcgatcg atgctctcac ctctgaagca
     9361 gcggccgaca cttcctctac agccgctgca ttctcctggg atacggcagc caccgaatcg
     9421 atcgatgcct tcacctcgcc gacccccgcc tccatttcct cggcagctgc cgcattgccg
     9481 tctgctacca ttacgatctc ttcgacagct ttctgaagac gagcgctagc agtaatcaaa
     9541 tcattcccgt acgcagccaa ttcctgaacg tatgtagcgg tctcgttggc atttgccaag
     9601 attttttcga gaaggcccgt agcccccgat gcattctccg cagcctccgc cgtagcagcg
     9661 ttggagactt ctatcgccgt aacgaccctg cccgtggaat ccctgagccc ctccacaagg
     9721 cctgcaatgg ccttagtctc agcaagcgac ctttctgcca gttttctgac ttcgtcggcc
     9781 accacagcaa atccacgtcc gtgctcccct gctctagcag cctcgatggc ggcattgagc
     9841 gcgaggaggt tggtctggtc cgcaatatcg ctgattacct cgactatcct tcctatctcc
     9901 cgggagctct catccagttc gcgcgcggca tcactgaccc gagccgcctc aacgcgcgca
     9961 ctttccacac tagtcaccat ggaatccacg gcggcacttc cttcagccgc agcctccttg
    10021 gtgctctctg tggcagaact cacattctca aggccatttg cggacttggc cagcgccgtc
    10081 cccatgtctc ccaacatcac agatgcctcg tgcacacttg tggtctgcga ctcagcgccc
    10141 gccgcaacct gcccgatagc catgctcagc tgctctgcag ccgccgcgga ctgctctgcg
    10201 ctatcactcc cctgcaaagc tgcggtggcc atctgctgga ccgtgtcgga tatctgttgc
    10261 atggcctccg cagactcctg catcgacgat gacaattgca gactcgcact tgccacaatc
    10321 tccgatgaac tgacggcatc gctcaccaga acgctcaacg aacccaccgt cctgttgaac
    10381 gcgtccgcaa ggtctccgat ttcatccctc gacttcacac gggtttcaac agtcagatcc
    10441 ccttcagcta cacgcttcac atccgctgtg agctggacta tgggaactgc gatcttcctc
    10501 gatgccgcca tcgccaagat gaccgccaag gccgcgcttg ctcctgtcaa cacagtcgcc
    10561 gccatctgca gggcatcgct tgcggcctca gccttttcca ctgcttcttc tatctttctt
    10621 tcctgcaagg agacgaattc atccaggacc tgttcccgcc ttaccctcac agggttgtat
    10681 tcttcagtga atatcgctgc agcttgaatc cgatcaaagg aagcgcaatc gatgatcttc
    10741 cgctcaactc gttcacgctc ctcgtttcct gcggcaatcc ccgctgcaat ctccttctcc
    10801 cttcccgact tgaatcggct tgtagcctcc cgcgtcgcgg tctccatctt cgtcaacgca
    10861 tcttcatagg cagcggcaaa aatcggttca tatggatcag agattatgcc gcaaaccgcg
    10921 ttcgatagca tcaggtctgc agttctgagc gattctgcag ttttcaaacc cattatttcc
    10981 tcagttgcaa ggcggcgaac ccctacttcg tacctctcca gcccgaagta gagcaacccc
    11041 gtggcgattc ccatcaccac tgcaaccgca aggaagcttg ccagaagctt cccccgaatc
    11101 ttgctgaaca tgtgttctat gcctccctat gcaaaagcac aatgtcaaac attagaactt
    11161 atcgatcttt agtattatcg gcatgccaaa gcaaactttt acccgcagtg cacacgggct
    11221 gttatccgca gcgatacacc gaaagttctg acccgtttca tcaccctccg cccaatacag
    11281 aagacccggc ggtgcaacgt gcgatcctca tcccttaaat gagaattgat ctatccccca
    11341 aatcatcggc gctgagggat tccatctgcg tgatcgcctt gtctggagtt taaaacctgg
    11401 ctgccctaaa gacgcttaag cctattccaa gtctgctccg ccgcagggaa gccatgccac
    11461 accgatggcc ccggggccag catgtgtccc aaccaccgga ccacactccg tcataatgat
    11521 ctcacgacaa ctgaagaggg actctatctt cacgcgccat tcgtctgccc tatcagcgga
    11581 gtctgcatcg attatggctg catgtatagg ctgcgctggt ccaatcttct ctcccatgaa
    11641 ctcgataacc cgtggcaaga tggacctccg tccgtatgcc ttggccacgg gcgtcacctc
    11701 accgtctatg atgctgacaa tcggctttac agacatcatg gttcccagca tggacgctgc
    11761 cttccctatg cgtccgtgcc gcgcaaggtg ttccagcgta tcaacggtga aaaagagcct
    11821 tgcagagcta atcccccggt atattgcgtc tcttgcgcca tccggtcctg cccctgccgt
    11881 cacagcccta gccgcggcaa gggctacaaa cccctggcac atggttgcct ggtgggtgtc
    11941 gatcacctct atcgatcccc tctccaacgt gtccctggct atgcaggcgg attggaaggt
    12001 cccgctcagc ttggaagaag cgtggataga gaaaatgtaa tcgtactccc tcagcagccg
    12061 atcatagagc gagatgaagc tggctggtga cggttgcgac gtagaaggtg ccgcatccga
    12121 agctttaagt ctcgcgaaga actcccgaga tgaaaggtct acgccatcga ggaaggattt
    12181 ctccccgaag atcacatgga ggggaaccac gaaaatcccg ttttcactgg ccacatcagc
    12241 agggagatcc gctatgctgt ctgtcacgat cgcaattctc atgatccgcc ctcctcatat
    12301 cctcccattt cccgcacctt ccgccccacc ttgcaacaac ggcagagttg cgtgcaacct
    12361 ccacgatctc gcacatattt ggacaccctg agcattcccg ggaggatgtt gtataagttt
    12421 gctccggaac ctcccatccc gcaaagagag tgcttctttt ggacgattcg atatggcgcc
    12481 gggccaacat cgccgcgcct atcgccccca tcacatcgaa atgctcgggc accaccacct
    12541 cgtaccccag cgcccgctcg aaagccctcc tcatcccgat atttgcggca actccgccct
    12601 ggaaaacgg
//
LOCUS       DAOSAI010000020        12503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3408_length_12503_cov_4.223811, whole genome shotgun sequence.
ACCESSION   DAOSAI010000020 DAOSAI010000000
VERSION     DAOSAI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12503
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3408_length_12503_cov_4.223811"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..889)
                     /gene="cas6"
                     /locus_tag="PLM83_01595"
     CDS             complement(143..889)
                     /gene="cas6"
                     /locus_tag="PLM83_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOK70134.1"
                     /translation="MRLNITLNPSGPVMLPVHYNHILQSAIYANLGPDFGTFIHDEGY
                     ERGSRIFKPLTFSRLRGSYRLDRAVGTISFDGKVHLVVCSPVEEICRSLGTSLLVKGF
                     ITLAGQKIPVECIEANNPVVLTDELTVVTLSPVVVYSTLTKGDGSPYTCYYQPGEKEF
                     EELVSRNLASKYEAFLGKQAPMGTVSVKPLRQPRLHVVYYKDTVIKGYSGPLLMTGPR
                     ELLLLALNAGLGSKNAQGFGCLAVVGHSRA"
     regulatory      1464..1686
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1844..3616
                     /gene="nrdD"
                     /locus_tag="PLM83_01600"
     CDS             1844..3616
                     /gene="nrdD"
                     /locus_tag="PLM83_01600"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOK70135.1"
                     /translation="MLEAQHEIHEAGHGESKWLDLMNAIGEIIENTSRENANIGNSPS
                     AKMLQIASSASREYYLDRVIPDDIACAHRNGDIHIHDLGYYGKTLTCVHIPLGRLLAE
                     GFDTGHGYVRPPKRPASAAALAAIIIQSCQNDMHGGQSFAHFDTDMGPYMEGFDEDEA
                     YQGMEAFIYNLNTMHSRAGAQVPFSTVNIGCDTSAGGRRVTRLLLEAYERGLGRGETP
                     VFPNIIFRVKSGVNMNPKDPNYDLFKLACRVCARRMNPTFSFMDSSFNAPYGDEVAYM
                     GCRTRVMANRRGQETSVGRGNLSFTTINLPRLALMAGGNVDRFFRLLDTKLELVYRQL
                     LHRFDIQSRLKVRDMPFLMGQKIYIDSYGLGENDEIGPAIANGTLSVGFIGLAETLTV
                     LCGAHHGENEEAEQLGREIVSFMRRQIDGFAEESNLNFTLLATPAEGLSGRFVAIDRE
                     RFGTVRNVTDREYYTNSFHLPVRYPTTISRKLVVEGAFHGLTNAGHISYVELSSPPIE
                     NGEAVESIVKAMARADMGYGGINFPIDECRKCGHAGVIESSCPSCGSGDISRIRRITG
                     YLAPLDRFNDAKKRELEERVPHGN"
     gene            3606..4145
                     /gene="nrdG"
                     /locus_tag="PLM83_01605"
     CDS             3606..4145
                     /gene="nrdG"
                     /locus_tag="PLM83_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOK70136.1"
                     /translation="METSVIDKLRSHASLDFHLRLAGIVDESVVDGPGVRLVVFAQGC
                     PHHCPGCHNPHTWATDGGVSVAAEEIIKRLRANPVLSGVTLSGGEPFAQASEFGRLAM
                     AVKRMGFTVTTYTGYKWEHLVQSSYSGVHLLLDNTDILVDGPYVDNLREPGLKWRGSS
                     NQRIIDVHRSLGAGSVVLL"
     gene            4415..5308
                     /locus_tag="PLM83_01610"
     CDS             4415..5308
                     /locus_tag="PLM83_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOK70137.1"
                     /translation="MVKLERLANKPILRPVPEHGWERAAVFNCAAVYKDGIFHLIYRA
                     CNLPSHDRFGPYISTMGHAVSLNGVDFARDDKPVFSGEGPQEQRGVEDPRIVELDGRF
                     YMMYTAFGARFDGDYRISMASSPDLINWSRHGVVLDEPNKDASLFPEKVRGRYAMYHR
                     RHPNIWMAFSDDLVNWTDHKVIMEIIPDSWESERIGIAGPPFRVAEGWALIYHGVDSE
                     NRYRLGWALFDGDDPTKLIYRQREPILQPELEWEREGWVPNVVFSCGQAVVDDTVYVY
                     YGGADACIGVAAAKIEDFTAK"
     gene            5358..6398
                     /locus_tag="PLM83_01615"
     CDS             5358..6398
                     /locus_tag="PLM83_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK70138.1"
                     /translation="MGVTIEDVARRAGVSASTVSRTLNNKGRISAETRRRVIEAAREL
                     EYPVSADAGLSRRRTNRIGILFDRRLHSLVADPFYGLVMVGVEEWLRDEGYQVFFSTF
                     SDREADMAMISEFAAERPVDGLIMAGCDIDLDCIRKAVFMGMPLVLVDNNIVEPKVPC
                     VMTDNTAGAREAVEHLISLGHEEIGFVSGPMTHSSLYERYQGYRQAMEANGLLVRSDM
                     VIAYEDVADYDVNGGYKAAERLLKRSPRPTAIFAGNDSMAIGVMKAAQELGLVVPDDL
                     SVVGFDDIELAAHTTPALTTVRVHKRELGYHAARLLLESIDGDPDRIPYKIAVCTELI
                     VRESTCPRRLDA"
     gene            6420..7343
                     /locus_tag="PLM83_01620"
     CDS             6420..7343
                     /locus_tag="PLM83_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK70139.1"
                     /translation="MKEAAVEERQPSYLARLRMEYRSSDKAGYIMVLPYVLHFLVFVA
                     YPLVFAFILVFCKWDIVSPMEFVGLRNIQRLMRDTTFWTAVTNTLKFLALHIPLQIVF
                     ALLIAVVLNGPIKLRGFFRGAFFLPFVISGAVVTILWQQLYSTDAGVFNLILTKLGAS
                     PVPWLTDPSIAIYSIAVMATWKNVGFYIVLFLAGLQNVPRELYEAASIDGANRGQQFW
                     KITMPLLRPVTLLVIMLSTIGGFGLFIEPFVMTGGGPAESTLSVVLYLYKNAFQFLKM
                     GYAATIGFALALMIFLVTLVQRKLLDVEQPY"
     gene            7360..8178
                     /locus_tag="PLM83_01625"
     CDS             7360..8178
                     /locus_tag="PLM83_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK70140.1"
                     /translation="MRTFKKILGYVILTAAALTFIYPFLWMISASFKPESEIGSLSLI
                     PKNFTLAGYGAVFTQIPILKALFNSVFVSVISTACVLIFSSMVGYALARLRFIGRDTI
                     FNLVLLTMMIPGQLTLIPMYVLIVKLGWADTYKALIVPAMMSNMGIIIFRQFFMSLPQ
                     ELVDAARIDGCSDYEILFKIFWPLSKPSLVTVGITTFIGSWNEVLWPLIVIRKWDMMT
                     MPQMVTLFSVGGLAGAQVAAEMAAATMLALPVVIAYLFFQNYFIESLALSGIKG"
     gene            8317..9621
                     /locus_tag="PLM83_01630"
     CDS             8317..9621
                     /locus_tag="PLM83_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK70141.1"
                     /translation="MRTSIKSRVAAIVAALLMVSLSCGIIEAKTPVTITFWPSSNPQE
                     IEFAKIIVARWNKQNPDIQVKMEPLPASRSTEEVLLAAIAAGTTPDVCANIYPGAISQ
                     YIAGKGLYPHDKLPGFYDFVYERSPKEIVDSFTYRDGHIYQIPWKANPVMLAYNVNLL
                     RENGIDPRSLSTYSGFLKAAEKLAKDTDGDGRIDQWLYAPSTDVTWWQRFFDFYTLYI
                     AASGGKTLLDDRGRVAFEDEAAVAVFTFLRTLFDKKLVPKGMLIGDQFLGGKVATTIT
                     GPYSIPYYDQNAPEGFKYDFMPVPVPDGHKGPVVTYGDPKNIGIFANSKHPLEAWEFV
                     KFIMSRENDLLWLEITSQPPYRKDIVDDPMFAPYFKKNPMMSKFVVQGAYTRGVDDSK
                     YLIEIFDAISKQYDLCCVNGRKTPKNAVRDGARDARDILAGW"
     gene            9630..10982
                     /locus_tag="PLM83_01635"
     CDS             9630..10982
                     /locus_tag="PLM83_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOK70142.1"
                     /translation="MTERTSERYVKSKRTARHVRALASCLAAGLILAGLMLAPCGGMA
                     AGGPVSIDFWCSSNPQEIEWAKAVVGKWNATRRDIVVNLQPLPASRSTEEALLAAIAA
                     KTTPDVCANIYPGVVTQFVAAGGLYRMDRFRDFIPFMEQRLPSSIMEQHRSPDGHFYQ
                     VPWKGNPIMLAYNVKMLREAGVDPASLRTYSGFLSAAKKLTRDRDGDGRVDQWMIYLN
                     IEPIWWQRFFDFYTLYVAASCGRTLIEGNRAAFNNRAGVAAMGFLAEIFKRGYAPKST
                     FPGDVFLQEKTATYITGPFAIPFWESMKPKGFEYDFVPVPVPDFYTGKEVWSYSDPKN
                     VGIFQTSKHPEEAWEFVKFMLSEENDAMFLAMTSQIPFRKNFDKVEGFASIAKKQPHL
                     AKFMVQSRYTQGADNVKPLVEIYDAIAQEYQYCAVLGQETPEEAIKIAEERVNEILSY
                     "
     gene            11034..>12503
                     /locus_tag="PLM83_01640"
     CDS             11034..>12503
                     /locus_tag="PLM83_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70143.1"
                     /translation="MNSARRRWALAAAACAFILMVAAFGASAAESGIEYASDAHRAVN
                     EMVNLSHLDYLRDEIVRSDGTVIPIWWVYCEPSVPGDRSSQYKYVEAASEGISCVDDV
                     ARAALAYLAEYERSGDEHALDMARDCFTFIEYMRTPEGHFYNFILESGSRNLKGATSE
                     KGVNWWTTRGMWALARGIRVFDKVDPEYAAHLEELIQPSLESIWEFVTAYELSKYGEY
                     KTLHGVKLPAWLVEDGSDASGVLVMALCELYEARPNQRTAEMAKMLADGIVGFQMGTP
                     DEFPFGAHLPWGGTITNWHGWGSHQIKALAMAGRIFGNQAWIESAEYAANSFVTHMLA
                     SIGMFAHMGPAPLSYVQLSYGAQTITTGLLELYRATGKEIYAVLGGIAGSWYMGNNVA
                     NHPMYDPATGRGWDGIDPPGPERGIGVSFNAGAESTIEAVTTMIELASVPKACEYVNL
                     ANRWKYAYRVVEAESFDNPLSGRPRKIWAQWTGENTPSGE"
BASE COUNT         2946 a         3241 c         3572 g         2744 t
ORIGIN      
        1 gttcctcgcg gacaggtttc caaccgaggc agccgattgt gttgcacgtt tgcctctagc
       61 cctctgctat gcttactgaa gcaatgatcg tcaaaacgaa atggacgggg taagaacatt
      121 gtgcatacga tccatacctt tgctaggctc gagagtgtcc aacaaccgcc agacatccaa
      181 atccctgtgc gtttttcgat cccagacctg cattgagcgc aagcaacagc agctctctcg
      241 ggccggtcat aagcagtgga ccagagtacc ctttgattac agtatctttg taatacacga
      301 cgtgcaatcg tggttgtcgt aaaggtttta ctgatacggt ccccatagga gcttgcttcc
      361 ccaggaacgc ctcgtattta ctcgccaagt tcctggacac gagttcctcg aactcctttt
      421 cgccaggctg atagtagcag gtgtatggtg atccatctcc cttcgtgaga gtactgtaaa
      481 cgactactgg cgacagtgtg actacagtta gttcatcagt aagcactacg gggttgttcg
      541 cctcgatgca ttcaacaggg attttctggc cagcaagggt tatgaagccc ttaactaaga
      601 gactagtccc caatgatcgg cagatctcct caaccggcga gcacactacc agatgaactt
      661 tcccgtcaaa cgatatagtc cccactgccc tgtcaaggcg ataagagccc ctcaagcgtg
      721 agaacgtaag gggcttgaat atccgagaac cacgttcata cccttcatca tggatgaacg
      781 tgccaaagtc aggacctagg ttggcatata tcgcagactg caatatgtgg ttgtagtgca
      841 ccggcaacat caccggtcca gaaggattca gagtaatatt gagacgcaaa tcaattcatc
      901 ctctgcgaaa ttactcaata tgtgggtgtt ccatgaaaat accgttctga tggccgtgtc
      961 aactgcagca tagatatcgg ttatgaatat tcgatatatt gtctcatccc cctcagccca
     1021 gctatagcca gaatatgggc tttcggccgc gttgtcgcat tatcagaggg tggcaacaag
     1081 aatgattcga ttgccaatga tgacgatatt gtctgaatca cacattcacc tgacaggtaa
     1141 tacccactac atactagtat accccacaag tgccaattac ccttcatgca acaagatccc
     1201 aatccgcatc gaattgccgt gttttccggg atctcagctt gtgcactcat gatttccagg
     1261 actgtgtatg caccgtgttt aggacgtgat cgccatgtac aaggcctgta cgcacggtat
     1321 tctgagagag atcacgccaa gccgatgatc tgtcgccatc ttacttgata ttgagggcgg
     1381 tagtttcggt tctgcacata aactgatata ctatcgctgc aagcctatca caacaacggt
     1441 ttgagtttgg atgggcaaac tgaatatcgc aaatcctgtc aggtgccctc atgcagaggg
     1501 agaataggga agaggcagca ggctgaggtt gtgcaaccgc tcctgcgaag cccgcggtcc
     1561 cgccactgta gtcagggagc cgaacgcgag gcagcgatgc cggtgtcact ttcgcaagat
     1621 gaagggaaga tgcgtgcagg cgatgatctg acagccagga gacctgcctg acaggatgcg
     1681 agccaccttc cgtggaaaga gggggcggcg cctgctccgc atattcgctc gccgagcgat
     1741 ggggagcaga gaatgttgct ggcggcgtgg cccccgtttg accgaaaacg ggggcctgtg
     1801 gttttacagg cagccaggag acgtatttag ggggcgctgg taaatgttag aggcacaaca
     1861 cgagatacat gaggcagggc atggagagag caagtggctt gacttgatga acgcgatcgg
     1921 agagatcata gagaatacca gcagggagaa tgcgaacatc ggaaactctc catctgccaa
     1981 gatgttgcag attgcatctt cagccagcag agaatattac ctcgatcgtg taatacctga
     2041 cgacatcgct tgcgcgcata gaaatggcga tatccacata catgacctcg gctactacgg
     2101 caagacattg acttgcgtac atatcccgtt gggaagattg ctggcagagg gtttcgatac
     2161 cggccacggc tatgtccgcc cgcccaagcg gcctgcctcc gccgcggcgc ttgccgcaat
     2221 cataatacag agctgccaaa atgacatgca cggtggccaa tcatttgcgc acttcgacac
     2281 cgatatgggt ccctacatgg agggctttga tgaagacgaa gcctatcagg gaatggaggc
     2341 cttcatatac aacctaaata ccatgcacag cagagccggg gcccaagtcc cgttctccac
     2401 ggtcaatatc ggctgcgaca cgtccgccgg cggacgtcgc gtcacaagat tgttgttgga
     2461 agcttacgag cgtgggcttg gaaggggcga aacgcctgtt ttccccaaca tcatcttcag
     2521 agttaaatcg ggggtcaaca tgaaccctaa ggaccccaac tacgaccttt tcaagctggc
     2581 atgccgagtg tgcgccaggc gcatgaaccc gacgttcagc ttcatggatt cctcgttcaa
     2641 tgcaccatac ggggacgaag tggcctatat gggctgcaga acgcgggtga tggccaatcg
     2701 cagaggtcag gagacaagtg ttggaagggg caacctctcc ttcacaacca tcaaccttcc
     2761 ccgattggcc ctaatggccg gcggaaacgt cgaccgcttt ttcaggctgc tcgataccaa
     2821 gctcgagctc gtgtacagac aattgctgca cagattcgat atccaatcga ggcttaaggt
     2881 tcgtgatatg ccatttctga tggggcagaa gatatacatt gactcatatg gacttggcga
     2941 gaatgacgag attggccccg ccatagccaa tggcacgctt tccgtaggtt tcatcgggct
     3001 tgcggagaca ctgacagttc tctgcggtgc ccaccatggt gaaaacgagg aagcagagca
     3061 gcttggccgg gagatagtct ccttcatgcg tcggcagata gacggatttg ccgaggagag
     3121 caacctcaac tttaccttgc tggcaacccc tgcagaaggg ttgtcaggcc gtttcgtggc
     3181 cattgacagg gaacggttcg ggactgttag gaacgtgact gatagggaat attacaccaa
     3241 cagcttccac cttccggtga gatatccaac gactatttca cgaaaactcg ttgttgaggg
     3301 tgcgttccat ggattgacca acgcagggca catatcatac gtagaactct cgtctccgcc
     3361 tattgaaaac ggagaagcag tggaaagcat cgtgaaggca atggccaggg cggacatggg
     3421 ttacgggggc atcaactttc ccatagatga atgccgcaag tgcggacatg ccggggtgat
     3481 cgagagctcc tgcccttctt gcggttcagg agacatctcc cgcataagaa ggatcacagg
     3541 ataccttgct cccttagaca ggttcaacga cgcaaaaaag agggagcttg aggagagagt
     3601 tccccatgga aactagcgtt attgacaagc tccgttcgca cgccagcttg gatttccacc
     3661 tcaggcttgc cgggatagtg gacgaaagtg ttgtggacgg gccaggagtg aggcttgtgg
     3721 tttttgcaca gggatgcccg caccactgcc cgggctgcca caatcctcat acatgggcga
     3781 cggacggggg cgtatcggtt gcggctgagg aaataatcaa gaggctgagg gccaacccgg
     3841 tactgtctgg agtgaccctc agcgggggag agccatttgc tcaggcttcc gaattcggca
     3901 gattggcgat ggctgtcaaa aggatggggt ttaccgtgac gacatatacc ggttacaagt
     3961 gggagcatct tgtccagtcg agctacagcg gagtgcacct gctcttggat aatacggata
     4021 tactggtaga cgggccctat gtcgacaacc tcagagagcc gggcctcaaa tggcgcggct
     4081 ccagcaatca gaggattata gacgtgcata gatcactcgg ggcggggagc gttgtgttgc
     4141 tgtaggtgag agccatttgg gcgtcaggcg cccgttttcc acccgccccc gcatccatcg
     4201 cacatccgat gcggctgttt ggcgccgcgg ccagccaaac agcgttgtct caattcagct
     4261 ccatatcatc attcctcttc ccaaaccaca aaacttttgc gcaaagaagg aaacctggat
     4321 gtaatgtcga atacatattg cgcaatagca atgcaatata tatgtgcaag tgcgcaaatg
     4381 ttgcgcaaga cgtgccaatt acggaggtgc gccaatggtc aagcttgagc gactcgccaa
     4441 caagcctatt ctcaggcctg tgcctgaaca cggatgggag agggcggcgg tattcaactg
     4501 cgcagcggtc tacaaagacg gcatcttcca ccttatctac cgcgcctgca atctgcccag
     4561 ccacgacagg ttcggaccct acatttccac aatggggcat gctgtgagcc tcaacggagt
     4621 agatttcgct agggacgaca aaccggtgtt ttccggggag ggcccccagg agcaacgggg
     4681 ggtcgaggat cccagaatcg tggaactaga cggcaggttc tacatgatgt atacggcctt
     4741 tggcgccaga ttcgatggag actatagaat atcgatggca agttcgcctg acctcatcaa
     4801 ttggtcgcgc cacggagtag tcctggatga gcccaacaaa gatgcctcgc tctttccgga
     4861 gaaggtacgt ggcaggtacg cgatgtacca cagacgccac ccgaacatct ggatggcctt
     4921 ttccgatgat ttggtgaact ggaccgatca caaagtgatc atggagataa tacccgattc
     4981 gtgggaatcg gagcgcatag gcattgcagg tccgcccttc agagtggctg aggggtgggc
     5041 cttgatctat catggcgtag attctgaaaa caggtaccgc ctgggctggg cgctctttga
     5101 cggagacgat cccacaaagc tgatttatcg acaacgcgag cccatcctcc aacctgaact
     5161 ggaatgggag agggaaggct gggttcccaa cgtcgtcttc agctgcggac aggcggtagt
     5221 cgatgacaca gtctacgtct actatggcgg cgcggacgcc tgcatcggcg ttgctgcagc
     5281 aaagatcgaa gactttactg caaaataggg gcaaaaaacg tgcgcaagtt gcgcaaaacg
     5341 gaacggagag tggcgccatg ggagttacga tagaagacgt cgcaaggagg gctggcgttt
     5401 cagcgtcgac cgtgtctcgg accctcaaca acaagggcag gataagtgct gagacgaggc
     5461 ggagagtgat agaagcagcc cgcgaactgg aatacccggt gagtgcggat gcgggcctct
     5521 cccgccgacg gaccaatcgc ataggaatac tcttcgaccg acgccttcat tcgctcgtcg
     5581 cagacccatt ctatggcctg gtaatggtcg gggtcgagga atggctgcgc gacgagggtt
     5641 atcaggtatt cttttccaca ttctcagatc gtgaggccga catggccatg atctcagagt
     5701 ttgccgcgga gcgccctgtg gacgggctga tcatggcagg ctgcgacatt gacctcgatt
     5761 gcatcaggaa agcggtattc atgggaatgc ctctggtgct ggttgacaac aacatagttg
     5821 agcccaaggt tccctgcgtg atgactgaca atacagcggg ggcgcgggaa gctgtggaac
     5881 acctgatcag cctgggccac gaagagatcg gattcgtctc aggccccatg acccatagct
     5941 ccctttatga acgttatcag ggctataggc aggccatgga ggcaaatggc cttttggtca
     6001 ggtctgatat ggtcattgcg tatgaagatg tggccgatta cgacgtcaat ggaggttaca
     6061 aggccgccga gcgccttttg aagaggtcgc ccaggccgac tgcgatattc gcaggcaatg
     6121 attccatggc gatcggagtc atgaaggccg ctcaggagtt gggcctcgtt gttccggacg
     6181 atttgtcggt ggtcggattc gatgacatcg aattggcggc tcatacaacc cccgccctca
     6241 caaccgtgcg agtgcacaag agggagcttg ggtatcatgc agcccgcctt ctcctagagt
     6301 ccatagacgg ggatccagac cgcatccctt acaagatcgc ggtctgcacc gaattgattg
     6361 tccgcgaatc aacatgcccc agacgtctcg atgcatagtt ttgtgtgggg gtgacttaag
     6421 tgaaggaggc tgcggtcgag gagaggcagc catcatacct tgcgcggctg cgcatggagt
     6481 accggtcgag cgacaaagca ggatacataa tggtgcttcc ttatgtattg cattttctcg
     6541 tctttgttgc atatcccctc gtatttgcgt tcatactcgt gttttgcaag tgggacatcg
     6601 tatccccaat ggaattcgtc ggactgcgta acattcaaag actgatgcgt gatacgacgt
     6661 tctggactgc agtcacgaac accctgaagt tcttggcttt acacattccg cttcagatag
     6721 tctttgcact cttgatcgca gtggtgctca acggcccgat aaagctgaga ggtttcttta
     6781 ggggcgcgtt cttcctgcca ttcgttatct ccggcgcagt tgtcacaatt ctttggcagc
     6841 agctttactc caccgatgca ggagtcttca acttgatcct gaccaagcta ggcgcatctc
     6901 ctgtgccatg gctcactgac ccgtctatcg ccatctactc tattgcagtc atggccacgt
     6961 ggaaaaacgt cggattctac atagtgctgt ttctggcagg gctccagaat gtgccgcgcg
     7021 agctttacga ggccgcgagc atagatgggg ccaaccgggg acaacagttc tggaagatca
     7081 cgatgccgct gctcaggccg gttacgctgc ttgtgatcat gctctctacg ataggcgggt
     7141 ttggtctttt catcgagcca ttcgtgatga ccggcggtgg gccggcagag agcactctat
     7201 ccgtggttct gtatttatac aagaacgcgt tccagtttct aaagatgggt tatgcggcta
     7261 ccatagggtt tgcgttggcg cttatgatct ttttggtcac gttagtccag agaaagctgc
     7321 tcgatgtcga gcagccatat tagagcggag gcggtagcga tgcgaacatt caagaagata
     7381 ttgggctatg taatcctgac cgcggccgct ctcacattca tatacccatt cctctggatg
     7441 atctcggcat cgtttaaacc cgaaagcgag atcggcagct tgagcttgat tccgaagaac
     7501 ttcacgctcg caggctatgg ggcggtgttt acccagatcc caatcctcaa ggccttgttc
     7561 aactcggtat ttgtctcggt tatatcgacc gcctgcgtcc tcatattcag ctccatggtg
     7621 ggctacgctc tggctcggct caggtttata ggacgtgaca ccatcttcaa cctcgtgctt
     7681 ctgacgatga tgatcccggg ccagctgacg ctaataccca tgtacgtgct gattgtgaaa
     7741 ttaggttggg ccgataccta caaggcattg atagttccgg ccatgatgag caatatggga
     7801 atcatcattt tcaggcagtt cttcatgtcg cttccccagg agctagtgga tgcggcgagg
     7861 atcgacgggt gctccgacta cgagatactt ttcaagatct tctggcccct ttccaagcca
     7921 tcactggtta cggtgggcat aaccaccttc atcggctcat ggaacgaagt gctatggccg
     7981 ctcattgtga tcaggaaatg ggacatgatg accatgccgc agatggtgac tttgttcagc
     8041 gtcgggggac tggcgggagc gcaggtggct gcggagatgg ctgctgcaac catgttggcg
     8101 ttgcctgttg taattgctta cctcttcttc cagaactact tcatagagag cctggcgcta
     8161 tcgggcatca agggatagca acgaaggcta gagccttctc agagctggtg gttggaaccc
     8221 gaaacaaaac cccttgtggt acgcacttta ctcctcgcgc aggagcgcgg gttcatatga
     8281 acaaacaagc gagattactg aaatggaggg acggcaatgc gaaccagcat caaatcgcgt
     8341 gtagcggcca tcgtggctgc tctactgatg gtgtcgttgt catgcgggat catcgaggcg
     8401 aagacgccgg tcaccatcac attttggccg tcgtctaacc cgcaggagat cgagtttgcc
     8461 aagatcatag tggcaagatg gaataagcag aatccggaca tccaggtgaa gatggagccg
     8521 cttccggcgt ccaggtctac cgaagaagtg ctgcttgcgg cgatagcggc aggcacgact
     8581 ccggacgtgt gcgcaaacat ctacccgggc gcgatatccc agtacatcgc gggcaagggc
     8641 ctttatcccc acgacaagtt gcctgggttc tacgatttcg tctacgaaag aagcccgaag
     8701 gaaatagtgg attccttcac ttacagagac ggccatatat accagattcc gtggaaggca
     8761 aaccctgtca tgttggccta caacgttaac ttgctccggg agaacggcat tgaccccaga
     8821 tcgctctcta cctacagcgg tttcctgaag gcagccgaga agctcgcgaa ggataccgac
     8881 ggagacggcc gcatcgacca gtggctctac gctcctagca cggatgttac gtggtggcag
     8941 cggttcttcg acttctacac cctctacata gccgcgagcg gcggcaagac actccttgac
     9001 gaccgcggca gagtggcatt tgaagacgaa gctgcagttg cagtcttcac atttctgaga
     9061 accctatttg acaagaaact cgttcccaag ggaatgctta tcggcgacca attcttaggc
     9121 ggcaaggtag ccaccactat aacgggaccg tactccatac cttactacga ccagaatgct
     9181 ccggagggct tcaagtatga cttcatgcct gtgccagttc cagacgggca caagggccct
     9241 gtagtgacct acggagaccc taaaaacata gggatcttcg caaattcgaa gcatccgctc
     9301 gaagcgtggg agttcgtaaa attcatcatg tcccgcgaga acgaccttct ctggctggag
     9361 atcacatctc agcccccgta tcgcaaggat atcgtggacg atcccatgtt cgcgccctat
     9421 ttcaagaaaa atccgatgat gtccaaattc gtcgttcagg gcgcctacac ccggggcgtg
     9481 gatgacagca agtatctgat tgagatcttt gatgccatat ccaagcagta tgacctttgc
     9541 tgtgtgaacg ggcgcaagac tccgaagaat gcagttcgcg acggcgcgcg ggacgctcgc
     9601 gatattctgg cagggtggta gtgcacgtga tgactgagag aacttcagaa agatacgtca
     9661 aaagcaagcg gacggcccgg catgtccggg ccctcgccag ctgtctggct gcagggctga
     9721 tcctggccgg attgatgctg gcgccttgtg gcgggatggc ggcgggtggc cctgtttcca
     9781 tcgacttttg gtgttcatct aatcctcagg agatcgaatg ggccaaggct gttgtgggta
     9841 aatggaatgc gaccaggcgc gatatcgtgg taaaccttca gccattgccc gcttcgcgct
     9901 cgacagagga ggcattgctt gccgcaatag cggcaaagac taccccggat gtgtgcgcaa
     9961 acatctaccc cggagtggtc acgcaattcg tggcggcagg cggattgtat cggatggaca
    10021 gattccgtga tttcatccca ttcatggagc aacggttgcc ttcctccatt atggagcagc
    10081 atagatcgcc tgacggccac ttctaccaag tcccgtggaa gggcaacccc ataatgctcg
    10141 cctacaacgt gaaaatgctt cgcgaagcag gcgtcgatcc cgcatcgctc aggacgtaca
    10201 gcggtttcct gtcagcggcc aaaaagctca cacgggacag agacggagac ggcagagtcg
    10261 accagtggat gatctacctc aacatagagc caatctggtg gcagagattt ttcgacttct
    10321 acaccttgta tgtagccgcc agttgtgggc gaacgctgat tgaggggaac cgggcggcct
    10381 tcaacaacag ggcgggagtt gcggcgatgg ggtttcttgc tgagatcttc aagcgcggct
    10441 acgccccgaa atcgaccttc ccaggggacg tgttcctgca agaaaagacg gctacataca
    10501 taaccggccc gtttgctata cccttctggg aatcgatgaa gcctaaaggg ttcgaatacg
    10561 acttcgtgcc tgttcctgtc cccgattttt acacgggcaa ggaagtttgg agctatagtg
    10621 atccgaagaa cgtaggcatc ttccagacca gcaagcatcc tgaagaagcg tgggagtttg
    10681 tcaagttcat gctgagcgag gagaacgacg cgatgtttct ggcgatgacg agccagatcc
    10741 cgttccgcaa gaactttgac aaggtagaag gtttcgcttc aatagcgaag aagcagccgc
    10801 acctcgccaa gttcatggtc caaagcaggt atacccaagg ggcggacaac gtgaagcctt
    10861 tggtggagat atacgacgcg atcgcccagg agtatcagta ctgtgccgtt ctcgggcaag
    10921 agactcccga ggaagctata aagatcgccg aggagagggt aaacgagata ttgagctact
    10981 gagcagccaa gctgttgcat cagtcgggct cagcagcata gggggatatg aacatgaatt
    11041 cagctaggcg acgctgggca ctggcggccg cggcctgcgc cttcatcttg atggtggcgg
    11101 cgttcggggc cagcgctgcc gaaagcggta tagagtacgc aagcgatgcc cacagggctg
    11161 tgaacgagat ggtcaacctg tcgcatttgg actacttgcg cgacgagata gttcgctccg
    11221 acgggacggt catacccatc tggtgggtat actgcgagcc ttcggttccg ggcgacagga
    11281 gctctcaata caagtatgtg gaagcggcga gcgagggcat aagctgcgta gatgacgtgg
    11341 ccagggcggc ccttgcatac cttgccgaat acgagcgtag tggagatgaa cacgccctcg
    11401 acatggcaag ggattgcttc acgtttatcg agtatatgcg caccccggag ggccacttct
    11461 acaacttcat cttggaatcg ggctcccgta acctgaaggg cgctacgagc gagaagggag
    11521 taaactggtg gaccaccagg ggcatgtggg ctctggcccg gggcatcagg gtattcgaca
    11581 aggtcgatcc tgaatacgcg gcccaccttg aggagctgat ccagccgtcc ttggagtcga
    11641 tttgggagtt tgtcaccgct tatgagctct ccaaatacgg ggagtacaag acccttcatg
    11701 gcgtgaagct tccggcatgg ctagtggagg acgggtctga tgcctcagga gtcctcgtga
    11761 tggccctttg cgagctgtat gaggcgaggc ctaaccagag gactgccgag atggcaaaga
    11821 tgctcgccga cggaatagtc ggttttcaga tgggtacccc cgatgagttt cctttcggcg
    11881 ctcaccttcc atggggtgga acgatcacca actggcatgg ctgggggagc catcagataa
    11941 aggccctagc catggctgga agaatcttcg gcaaccaggc gtggatcgaa tccgcggaat
    12001 atgcggccaa cagcttcgtc acgcatatgc tggcgtcgat agggatgttc gcgcacatgg
    12061 gcccggcacc gctctcctac gttcagctgt cgtatggagc tcagaccatc accacgggct
    12121 tactcgagct ttaccgcgca acgggcaagg agatctacgc agtgcttggc gggatcgcag
    12181 gatcgtggta catggggaac aacgtggcga accacccgat gtatgatccg gccacagggc
    12241 gcggatggga cgggatagac ccgcccgggc ccgaacgcgg gataggcgtg agcttcaatg
    12301 ccggagccga atcgactatc gaggctgtaa cgacgatgat agagcttgct tcagtgccga
    12361 aggcatgtga atacgtaaac cttgccaaca gatggaaata tgcataccga gtggtcgagg
    12421 cggagtcctt cgataacccg ctatcgggcc gccctcggaa gatctgggcg cagtggacag
    12481 gggagaacac gcccagcgga gag
//
LOCUS       DAOSAI010000021        12213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3503_length_12213_cov_4.062264, whole genome shotgun sequence.
ACCESSION   DAOSAI010000021 DAOSAI010000000
VERSION     DAOSAI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12213
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3503_length_12213_cov_4.062264"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2331
                     /locus_tag="PLM83_01645"
     CDS             <1..2331
                     /locus_tag="PLM83_01645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK70144.1"
                     /translation="KLSPGASIFATTATRDLATVLLYDSVKVAEFREGLQLFDADDAQ
                     QALDRVVTYGFGTSFEAAEGIQAVLLQAGHILGAAAVLLQTEEGTLLYTGDFTTFEQE
                     TVGMQAFSGALKRGADVVITEATYGSRIHAPRSQEMQRLLDTIGETLDAGGRALVPAF
                     AVGRSQEIILALRNYMRRTKRKYPVYVDGLIRNVNTVFARNPNYLKQRYKKEVLRGHE
                     LFYTNGIEAVGSKSQRDKILASKDPCVIIASSGMLTGGVSPIYAERILEDEKNLLAIV
                     GYQDEESPGRRLLDLAELRKNERTILLNENEYRVRCKVGKFGLSAHADSLGIIASVKA
                     LRPDFVLLNHGNEESLEALSQALADELPDTRIEVAGSGGVYEYVPAPGGGKRRYSLKP
                     QVRRIALHKEEPVDPEELWQHLFQTGTLGTTLTVADLMMVWYGPREISDEEKRAFRKE
                     VSGSPRFRSAYTNPNIVYVLTEEEYVDAVTPKPMEQNEAWALIQERLSPFGLQKVGFA
                     SEGSVTLYFPTPKYAERCTDEIGELEREILRRIEVAPNSNMEFLKTKIKTDLAEEFGI
                     RVNRDPSFGAQSVTVKEPAGLAQPGIRGRTLGEYAEAFEEETGYKLLFRVEGQSLLDQ
                     VAARRSQDPWTPEGVPSHGVSEPMEQNMAKGMIDDAFAGAVNQPKVSIYRDEGRMALA
                     FITPQVGERYKEILDKLEAATGWRLEIQESARVNELADLARELLAANGLGGMKVGVHS
                     GYAEARGPVDVDEAVAQRLSKAYSELTGYELRFRRT"
     gene            2396..4021
                     /locus_tag="PLM83_01650"
     CDS             2396..4021
                     /locus_tag="PLM83_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK70145.1"
                     /translation="MKAVYGSALLTKPRPSWTLTMAWALVVAALSGHAAAGEIEAELV
                     LSPQARAIVETNRPIWPGPSGDVIEREAIELLYNYTGLEDEIDVAQIWRNQGRSVRTG
                     QSHQWHISPHYAAEEVELLFEVLKYGYAGYEYFGGDEAFEAAKDGILREIGEFAEEIP
                     MWQYIDIIAANLSFVRDGHLSIGERRICEYHQFRASPTHSFCMDNEGQFWNADGERIL
                     SIDGQSPSAFMKPSIDEAGDIVYLLGMVLPEGNGDVALKLEYADGCDEMAWLVPFSPM
                     VLDGPVYELSEVGGVPLITCGDFRDLPGQRRAIARFAEDAEGFRDEDVIILDLRSNRG
                     GRYAYGREWCRRLSGGDVGPSTYSRRLVTDVALALLSNTLPESDDNLSSLDNQDDAPW
                     MKDRSEDPELPGWSYAVVEQEARAGRTPFVVVLLDSYVASAAEVFVRRLRYCQNTVFI
                     GTNTRGAALCGNAGVMELPYSGLKVSLPTMICFDGEMQNIDGCGYFPDFWVHPDFVME
                     RAVRFIEKQLVSVQADGALGQTSEEEPGGIMRQ"
     gene            4064..5638
                     /locus_tag="PLM83_01655"
     CDS             4064..5638
                     /locus_tag="PLM83_01655"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK70146.1"
                     /translation="MRNPLHLRESRLFQALMAFCVIAIVTPVQPVLASSRTRLVLSPE
                     AKAIVEANRPELSEASREDLLARFSSMLYEYSDFEDEAYPSALRWDTAAGRESVMEEW
                     SISPARAVQEVDLLFHALKYGYAAYQFFGGDEVFCAARERMLDEIGRHYHDIPFWQYS
                     QIIIANLKFIQDGHFAFGSRTLCEWSTFRVSPYLRFSLDEDGFFINDGGKRVASVSGQ
                     APSSFLKPSIDEDGNIVYVLGMLVPEQQVDLMCEIEYADGSREIAWLVPIISMDYGGA
                     VYELGEIEGFPLVSCRSFDPDHDEGKALNRFVEDAEKLRQEDVILLDLRSNHGGSTIY
                     GMLWCQRLTKGNVGPYFFTAELATDTSIALLLNGLSERLGEELSSDQAEALWRYWKNA
                     EDPDRPGWRRIGIEQGGHVSDAPFIVALVDSNAGSATEAFVRRLRSLDNVVIIGTNTR
                     GALLCGNPGMLVLPYSGFEVRIPTMFWLDSEMHNYDGVGYSPDFWVHPDYALERAVKF
                     IRRHLVREGASVSLGG"
     gene            6215..7165
                     /locus_tag="PLM83_01660"
     CDS             6215..7165
                     /locus_tag="PLM83_01660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOK70147.1"
                     /translation="MKKLFIMGDGRLRPEWRLIVVLILELLITAMLFRVLWDLTMLSL
                     KVPPGLAPDGRLEFLMETIAGDELLGLASQLIPSIALILAILLALRFIDRKSIEDIGA
                     GFCARDRKELAFGLGLGALSMTVIFAVLLASKSITLENDLAHPMLSWNLAIGMVVFIF
                     VGLSEELYSRAYSISALSEIYNRWSAAIVSSVLFSLLHAGNANVSMGGLANVLLVGIL
                     FAYMFLRSGSVWMPVGYHITWNYFQGYVFGFPVSGAEVEGAYTVAASRNDFLTGGAFG
                     PEGGLLATTAILAGFYIVWRFTGGGNPWRTREPEGDSEFL"
     gene            complement(7368..8468)
                     /locus_tag="PLM83_01665"
     CDS             complement(7368..8468)
                     /locus_tag="PLM83_01665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK70148.1"
                     /translation="MAFDTINLSGLTAPGLVLDVGGGGEGIIGSVLGRQVVAIDILRE
                     ELEESSNDALRLVMDARSMQFLDGSFDEATCFFSLMYMSPESIPRVLSEIFRVLAPGG
                     RLRIWDVEIPPAKPGDDLFIIELKIVTGAGATTCAYGVGWPDKSQNCSAIAHVAQDCG
                     FELLGQAESGQTFYLSLLKPYSNVCEIPTKAGPMCIMRATAKHMHDAYGVLVDSCRRV
                     VERGKRVPFWLFTAEGQQHVAEKIRDCDYFIGFISGSPAAVLWIKWADSCSWEDAGSD
                     RLAGYVYGFGVKQEWAGAGVGRALLEWASSYIASKGRRLVRLECDAGNPTLCAYYEML
                     GFEDRGLTAPGNQLRKYERPSGSKAQCSIANP"
     gene            10101..10538
                     /locus_tag="PLM83_01670"
     CDS             10101..10538
                     /locus_tag="PLM83_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOK70149.1"
                     /translation="MGITRWDPFDDLVALRDAFGRVLGDTSLRTGFSGIGRWRPSVDM
                     YETNDNVVVRADLPGVDRDDLEVTVTDNTLTIKGQSRHEHEIESGNVFRRERAYGQFT
                     RTLPIDAGIRADEAKASFNQGVLEIVIPKTEESRVRTRRIDIN"
     gene            10757..11287
                     /locus_tag="PLM83_01675"
     CDS             10757..11287
                     /locus_tag="PLM83_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK70150.1"
                     /translation="MTKNVIEAIEERRSVREFKPDPIPDSIIARILECGLKAPSAGNV
                     QPWQFWVVSRPSLKSALAEAAFGQESVEQAPVVIVVLAEPSRSAARFGYRGSKLYCIQ
                     DTAAAVQNMMLAATAYGIGSCWVGAFDEKMVSKALGLSERFRPMALVPMGYPAEVSQR
                     RPRSLRPLAETVVTVE"
     gene            11486..>12213
                     /locus_tag="PLM83_01680"
     CDS             11486..>12213
                     /locus_tag="PLM83_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOK70151.1"
                     /translation="MDPRLEERHFLRPSVVYARGRVPVEAAFVARPNRFVAIVRLSCD
                     VEHAYGLDRAGAEITAHVADPGRLEELLIPGAKVYVLPAFPKGMDTMDYSPTLAGVGR
                     KTLYDLVLVDYEGILVSIDSRVPNELVHRALQAGFFPELAEYHQIVRESAFGSSRFDF
                     HLSAPGQVIGTQAAILDENISDCFVEVKSVTLVEDGLAKFPDAPTARGARHMRELAQA
                     VSAGLRGVVIFVIQRNDASNFTPN"
BASE COUNT         2741 a         2923 c         3694 g         2855 t
ORIGIN      
        1 aaactttcgc ccggggcatc catattcgcc acgacggcaa cgcgcgattt ggccacagtc
       61 ttgctgtacg attcagtcaa ggttgcagaa ttcagggaag ggctgcagct cttcgacgca
      121 gacgatgccc agcaggccct agacagggtg gtcacatacg ggttcgggac ttcttttgag
      181 gcagcagagg ggattcaggc ggtgttgctc caagcagggc acatcctcgg cgctgcggcg
      241 gtactgcttc agacggaaga agggacgctc ctttacactg gcgactttac cacatttgag
      301 caggagaccg taggtatgca ggcgttcagt ggagctctca agcgcggggc cgatgtggtg
      361 atcaccgagg ccacctatgg atcgaggatt cacgctccga ggtctcagga gatgcaaagg
      421 ctgctcgaca ctatcggcga gacgctggac gctggcggaa gggcgcttgt gcctgccttt
      481 gccgtgggaa ggtctcagga gataatcttg gctctcagaa actacatgcg gcgaacgaag
      541 agaaaatacc ctgtgtacgt tgatggtctg atacgaaacg taaataccgt tttcgctagg
      601 aacccgaatt acctgaagca aagatacaag aaagaagtcc tgcggggcca tgagcttttt
      661 tacacgaatg gtatcgaagc agtggggagc aagtcccaaa gggacaagat cctcgcatcg
      721 aaagatccgt gtgtgataat tgcgagttca gggatgctca caggcggagt gtcgccgata
      781 tacgcagaga ggattctcga ggatgagaag aatctcttgg cgatagtggg gtaccaggat
      841 gaagaatcgc cgggaagaag attgctggac ttggctgagc ttcgaaagaa tgaacggacg
      901 atacttttga acgaaaacga ataccgggtt aggtgcaagg tggggaagtt cggtctttcg
      961 gcccatgcgg actcgctcgg gatcatcgct tcagtaaagg ctttaaggcc cgacttcgtc
     1021 ctcctcaacc atgggaatga ggagtctctt gaagcgctct cccaagccct ggcagacgaa
     1081 ttgcctgaca cccgcataga agtggccggc tcgggtgggg tctacgagta tgttccggcg
     1141 ccgggcgggg gaaaacgacg atactccttg aagccgcagg tgcgcaggat agcgttgcac
     1201 aaagaggagc cggtagatcc ggaggagctt tggcaacacc tgttccagac agggactctg
     1261 ggaacgacgc tcacagttgc ggatttgatg atggtgtggt acggtccccg agagatctcc
     1321 gacgaagaga aacgggcttt ccgcaaggaa gtcagcgggt cgccgcgctt caggtccgcc
     1381 tacaccaacc ccaatatcgt gtacgtgttg actgaggaag agtatgtgga cgctgttacg
     1441 ccgaagccga tggaacagaa tgaggcgtgg gcgctgattc aggaaagact gtcgccgttt
     1501 ggacttcaga aggtgggatt tgcttcagag ggaagcgtga cgctgtattt tccgacgccg
     1561 aaatatgccg agaggtgcac agacgagatc ggagaattgg agcgggagat actgcgcaga
     1621 atcgaagttg cgccaaactc caatatggaa ttcctgaaga ccaagatcaa gacggacttg
     1681 gcagaggagt tcggcataag ggtcaaccgc gatccgtcct tcggcgcaca atcggtcact
     1741 gtgaaggagc cagcagggtt ggcacagcca ggaatccggg gacgaacgtt gggagaatat
     1801 gcagaggcgt tcgaggagga gacaggctac aaattgctgt ttagggttga aggacagagc
     1861 cttctggatc aggttgccgc acgacgtagt caggacccgt ggacgccgga gggtgtaccg
     1921 tcacacggcg tgagtgaacc gatggagcag aatatggcga agggcatgat cgacgatgct
     1981 ttcgcgggag cggtcaatca accgaaggtc agcatttaca gggatgaggg acggatggcg
     2041 cttgccttca taacccccca ggtcggggag agatacaagg agatcctgga caagctcgag
     2101 gcggcgacag gatggagact ggaaatccag gagagcgcga gagtgaatga gctggccgac
     2161 ctggccaggg agctcctggc cgccaacggc ctaggaggga tgaaggttgg cgttcattca
     2221 ggctatgccg aggcaagggg ccctgtggac gttgacgagg cggttgccca gaggctgagc
     2281 aaggcctact cagagttgac aggttacgag ctgaggttca gacggacgta gaggctgtac
     2341 cacttcgtgg ttgatgggtt ttgccgggga agcgaaacta cacgggggca ttacgttgaa
     2401 ggctgtttat gggagtgcat tattgacgaa acctcgccca tcttggactt tgacaatggc
     2461 gtgggcgttg gtggttgccg cattgtctgg gcatgctgca gcaggagaga tagaagctga
     2521 gcttgttctg tcgcctcagg caagagccat agtggagaca aacaggccga tttggcctgg
     2581 gccgtctggt gacgtcatcg agagagaggc catagagctg ttgtataact acacaggcct
     2641 ggaagatgag attgatgtag cccagatttg gcgaaatcaa ggccgatcgg tcagaaccgg
     2701 ccaatcgcac caatggcata tctctccaca ttatgctgcg gaagaagttg aactgttgtt
     2761 cgaagtattg aagtacggat atgccgggta cgaatacttc ggcggcgatg aagccttcga
     2821 agcggcaaag gacgggatac tccgcgagat aggcgaattc gcggaggaga ttcccatgtg
     2881 gcaatacata gacatcatcg ccgcgaatct cagtttcgtt cgggatggac atctctcaat
     2941 tggggagaga agaatctgtg aataccatca attccgggca agtccgaccc acagtttttg
     3001 catggataat gagggtcaat tctggaatgc cgatggcgag agaattctat ccatagatgg
     3061 ccagtccccg tctgcattca tgaaaccgtc tatagatgaa gccggcgaca tagtctacct
     3121 gttggggatg gttctgcctg agggaaacgg ggatgtcgcg ctcaaactcg aatacgcaga
     3181 tgggtgcgat gaaatggcct ggcttgtccc tttttcaccc atggttcttg acgggcctgt
     3241 ctatgagctg tccgaggttg gaggcgtgcc gctcataacg tgcggagact ttcgtgatct
     3301 tcccggccaa cggcgcgcca ttgctcgatt cgccgaggat gcggagggtt ttcgggatga
     3361 agacgtgatc atcttggacc ttaggtccaa tcgcggggga aggtatgcct atggtaggga
     3421 atggtgtcgc cggttgagcg gtggagacgt tgggccgagc acctattcca ggcgactcgt
     3481 tacagatgtg gcattggcat tgttatccaa cacgttgccc gaatcagatg ataacctgtc
     3541 tagtctcgac aaccaggatg atgcgccttg gatgaaggac agaagcgagg accccgagtt
     3601 gcccgggtgg agctatgcag tcgtcgaaca ggaggcccgg gcaggtagga cgccatttgt
     3661 tgttgtgctc ctcgattcat acgtggccag tgcagccgaa gtcttcgtca gaaggttgcg
     3721 atattgccaa aatactgtct ttatcgggac gaatacgcga ggtgctgcgc tttgcggcaa
     3781 tgcgggggtg atggagcttc cttattccgg gctgaaggtg agcctgccta ctatgatctg
     3841 ttttgacggc gagatgcaga atatcgatgg atgtggctat tttcctgatt tctgggtgca
     3901 tcctgacttt gtgatggaga gggctgtgag gttcatcgag aagcagctag tgtcggtgca
     3961 agccgatggg gccctcgggc agacgagcga agaagaacct ggaggcatta tgcggcaata
     4021 acgattatcc ggaatccata tgatttcttc gtgaggtgag gtcatgagaa atcccctaca
     4081 cctgcgcgaa tcacgtctat tccaggctct catggcattc tgcgtaatcg ccattgtgac
     4141 gcctgttcaa cctgtgttag catcatccag gacgcgatta gtcttatcgc cggaggcgaa
     4201 ggctatcgtg gaggcgaaca ggcctgagct ttccgaggca tctagggagg atctgttagc
     4261 gcgtttttca agcatgctgt atgaatacag cgatttcgag gatgaggcgt atccgagcgc
     4321 actcaggtgg gacacagctg cgggccgaga gagtgtcatg gaggaatggt cgatcagtcc
     4381 ggcaagggct gtgcaagagg tagacctctt gttccacgca ctgaagtatg gttatgctgc
     4441 ctatcagttc tttggtggcg atgaagtctt ctgcgcggcg cgggagagga tgctggatga
     4501 gataggccga cattaccatg atattccgtt ctggcagtat tcccagatca tcatagctaa
     4561 cctaaagttc atccaggatg gccattttgc ctttggaagc aggacattat gcgaatggag
     4621 cactttcaga gtcagtccgt atcttcggtt ttcactggat gaagacggct ttttcattaa
     4681 cgatggcggc aagcgcgttg cttcagtcag tggccaagct ccgtcttctt ttcttaaacc
     4741 gtcaatagat gaagatggaa acatcgtcta cgttcttggg atgctcgtcc cggagcaaca
     4801 ggttgatttg atgtgtgaga tagaatacgc agacggcagc agggagattg catggctggt
     4861 gccaatcatc tccatggatt acggcggagc ggtctacgag cttggagaaa tagagggttt
     4921 ccccttggtg tcttgtcgca gcttcgatcc tgatcatgat gaagggaagg ctctcaacag
     4981 gtttgtggag gacgcggaga agttgcgaca agaggatgtt atcttgcttg accttaggtc
     5041 aaatcacggg ggatcgacga tctacggcat gctttggtgc caaaggttga cgaaagggaa
     5101 cgtgggcccg tactttttta cagcagagtt ggcgactgac acctctatcg ctctgctcct
     5161 gaatgggctt tcggaaaggc ttggggagga gttgtcttcc gaccaggccg aggctttgtg
     5221 gagatactgg aagaatgccg aggacccaga taggcccgga tggcgtcgca taggcattga
     5281 gcagggtggg catgtcagcg atgccccgtt catagtagca cttgtggatt ccaacgccgg
     5341 gagcgcgacg gaggcttttg tccgtcgtct gcggagcttg gataatgttg taatcatcgg
     5401 aactaacacc cgaggcgctt tgctttgcgg aaatcccggt atgcttgttc tgccctattc
     5461 aggtttcgag gtgagaatac ccacgatgtt ctggctcgac agcgagatgc acaattacga
     5521 cggcgtcggg tattctccgg acttctgggt tcatccagac tatgcgttgg aacgggccgt
     5581 gaagttcatt cgcagacatc tcgttagaga aggggccagt gtctcacttg gcggttgatg
     5641 gaatcgaacg tctaaagaac cgggtcttcc cgttgcctgc tgcatgcgtt gggggattgc
     5701 tctttacatt ggcattggtc ttctcatccg ccgacacgtg tgggatgtcc gggcaagagc
     5761 tttatccgct accaacccaa aacttcgaca ttgggtcctg gccgcagaag caggatggag
     5821 agggtgcgga ggaacgccgt cgagaagctc aagggaatat gtaggtatgc gagctcgctg
     5881 atgcaatgat actcggcccg cgcctgacag gcactatagg gatgggggag atccggcggt
     5941 gttcgagtgc gctatcggcg ctacagctga ggcggggcag aaggtgtccg tcttcagctc
     6001 gaggagtgcg ggaaggagct tagtgaggcc atgacgttag atatcggtcc gcaggttcta
     6061 tgggagcact aggctttgct ggtggggcat cgcctcaaac gggaaaatcc aaaagccgct
     6121 ttatctctgc cgtggcctgt tacttgaata cgtagactgc gatcttttcc cgaggctatt
     6181 catgaaaact gcagatgact acggggggtt gtccttgaaa aagctattta tcatggggga
     6241 tggccggctc aggccagaat ggaggctcat tgtcgtattg atacttgagt tattgataac
     6301 ggcaatgctc tttcgcgtcc tctgggatct gaccatgttg tccctgaagg ttcctcctgg
     6361 cctggctccg gatgggaggc tggagttttt gatggaaacg attgccggtg acgagctcct
     6421 tgggcttgca agccagttga tcccttccat cgccttaatc cttgcgatcc tcttagcttt
     6481 gaggttcatt gacagaaaga gtattgagga cataggagcg ggattttgcg caagggatcg
     6541 caaggaacta gcctttgggc tggggcttgg cgctctttcg atgacggtca tcttcgccgt
     6601 cttgctcgcc tcgaaaagca tcaccctcga aaacgatctt gcccacccta tgctctcatg
     6661 gaatctggcg attgggatgg ttgtcttcat cttcgttggg ctgtcggagg aactctactc
     6721 gagggcgtat tcgatatcgg ctctgtctga gatatacaac cgttggagtg cggccattgt
     6781 atcgtccgtc ctcttttctc ttcttcacgc aggaaacgcc aatgtgtcca tgggcggact
     6841 ggcaaatgtg ctattggtag ggatcttgtt tgcctacatg ttcttgcgga gcggcagcgt
     6901 gtggatgccc gtgggttacc acatcacctg gaactatttc cagggctatg ttttcgggtt
     6961 tccggtgagc ggggcagaag tggagggtgc ctacactgtt gcagcaagcc gcaatgactt
     7021 tctgacaggc ggggcgttcg gccctgaggg cgggttattg gcgactacgg cgatcttggc
     7081 aggtttctac atagtatggc ggttcaccgg gggcgggaac ccgtggcgca cgagagagcc
     7141 ggaaggcgat tctgagtttc tgtagcaatg cgggaaggct tagtttgaac atgatcagcg
     7201 gatgagtctg atcaaggcga atgttattcc tgactgacca gcgaaggatt tgattgaaga
     7261 tgttgggcga tatacggttt ttgagcgtga cccaaagaag acggcaaggc tgcttgcggc
     7321 cgtgcccgga ttgtagatac tgcgaaggtc cggtactatg agttatatta gggattcgca
     7381 atcgagcact gggcctttga ccctgacgga cgttcgtact ttcgcagctg attgcctggg
     7441 gctgtcagtc ccctgtcttc aaatccaagc atttcgtagt atgcgcaaag agttgggttt
     7501 cctgcatcgc attccagtct gactagccgc ctgcccttgg aggctatata agaagatgcc
     7561 cactcaagga gggctcgccc gacgccggcg cctgcccact cctgcttgac cccgaagcca
     7621 tacacatacc ctgctaagcg gtcgctgcca gcatcctccc acgagcaaga atcggcccac
     7681 ttgatccaga ggactgctgc gggagaacca ctgatgaagc cgatgaagta atcgcagtct
     7741 cgtatttttt ctgctacatg ttgttggcct tctgcggtga aaagccaaaa gggaacccgc
     7801 tttccgcgtt ccacaactcg ccggcaactg tcaacaagga ctccataggc gtcgtgcatg
     7861 tgttttgcgg tggctcgcat gatgcacata gggcctgctt tagtcgggat ttcacagaca
     7921 ttagaataag gcttcagcaa gctcaagtag aatgtctgtc ccgactcggc ttggcctagc
     7981 aactcgaagc cgcaatcttg agcgacatgg gcaatagcgg agcagttttg ggatttgtcg
     8041 ggccagccca ctccgtaggc gcatgttgtg gcgccggcgc ctgtcacaat cttcagctcg
     8101 attatgaaca gatcatcgcc gggctttgca ggaggtatct ctacgtccca tatccggagc
     8161 cttcctcctg gcgcaagcac cctgaagatc tccgagagga cccgtgggat cgattccggg
     8221 gacatgtaca tcaggctgaa aaagcaagtc gcctcatcga aggacccgtc caaaaactgc
     8281 atgcttctcg cgtccatcac tagccggagc gcgtcattgg atgactcctc cagctcctcc
     8341 ctgaggatat cgatggcgac tacttgtctt ccgaggacag agcctatgat tccctcgccg
     8401 cctccgccca cgtccagaac gagacctggc gcggtcagcc ctgacaggtt tatggtatcg
     8461 aaagccatgg tctaatcgcc cctccatcac tcaaggatgt cgcaattgca gagcactttc
     8521 attatggttc cacttttata gtccggttcc tgcgagcata cagaatctat aagggctgca
     8581 agttctgagt agaccgcatt acatataacg agtgtgccat cagcataggc cttatcgatt
     8641 agccctttgg tagcgcatcg gggagcagga tgccgacaag agcgtctgtg tcaacagagc
     8701 tgatcacgca tgccctctca ttactctgtt ggtgtcggtg tcagcccctc tcggatatcc
     8761 cacataacga tcgaatggag aatcctcaac atacttgctg agaatgtagc cttgggggga
     8821 gtctcgtata cccgtgatcg agccctgtct ttccgcgaac gtctatagac gtgctatgaa
     8881 cggccaatag taaaaagcaa atctgcacct tgataactga acctgctggc ctccatttgc
     8941 catccggacc atatggctga ttgcgaattc aaacgaaata aaatgaggtg aactagggct
     9001 cctgttccta gacccgtgat ttttcatggc caaaagggga ttctcaaatg gccattgaca
     9061 atatgaccct acggagttct ggcgagcccc gtggttcctt gattcgctgt cgcatcgatt
     9121 tctgcagatg ccaccacggt gtatgtcacc gattttccca atgatggccg cggcgacagt
     9181 agcttctagg agccccggag catgctgcga ttgcagttcc tccatggtaa gcctgatgca
     9241 ggaaatctga ggctctatga actagatctg gctgaggatt agtttcaatt caaaggccat
     9301 ccacagctaa tcaaatagct gaaggtttct ctggcgagct cttgggtgca ccgagcgtgg
     9361 cccttgagct tattgaggtc aagcgctgcg attgtttccg gtgtagcatg gccttttagc
     9421 aactcttgag atgtctcgat gaagcaactg gcatactcag ggaagatgcg atctagaacc
     9481 cgatagcgcg ttggcctccg aggccatgaa ggtctcttca gtctggttga gacgtagcag
     9541 gttggcgagg atgaagggtc gatcttgctc tttgatatgg cgtcagagtg gatgggttaa
     9601 tcacatggac tcggtatccc tgtagcggca gccagtaggc gccgctaacg cgcttgcgct
     9661 cagctttggg ctgcgtgttc ggaatagtca tcattgacac aagcttcgtg ctgcttcttg
     9721 gcgacgtcga taccggtcaa gtcagtagta gacaacctag ctgtttactc gagacatgtg
     9781 ggtataccgc attcccaacg tgctcattcg aaaatccttg tagaggcggg gtaatactct
     9841 atcgggcttc ccagtcaatg aaactaccaa gacacgttgg ctttccgaaa catgactcct
     9901 ctttggttgt caaggatcat ctggggcctg tactagggaa cttcattctc ttcgtgtttg
     9961 agtcgcgcgc gtcggagtaa tactacctac tgcaaacatg gcgggaagcg ggcggcgtta
    10021 gccggaggcg agcagccttt ttggtcttgg ggagctttgc gggtgcaggg aaatatgatg
    10081 tggtttggga gggctgatag atgggcatca caagatggga tccattcgat gatctcgtcg
    10141 cgcttcgcga tgcgttcggt agagtgcttg gggatacgag cctgaggacg ggcttctcag
    10201 ggataggccg ttggcgtcct tcggtggaca tgtacgagac caacgacaac gtcgtagtca
    10261 gggcggattt gcccggagtc gatcgggacg acctcgaggt gaccgtgaca gacaataccc
    10321 tgacaatcaa ggggcagtcc aggcacgagc atgagatcga atcgggaaat gtctttcgga
    10381 gggagcgcgc ctatggacag ttcacgagaa cgcttcctat agatgccggg attcgggcag
    10441 acgaggccaa ggccagcttc aatcaggggg ttctcgagat agtcattcca aaaacggaag
    10501 agtcgagggt gagaacgcga agaatagaca ttaactagca gcaacgcaat gccgaacaga
    10561 ggctacggct gcccggcccg cccgtataat ccccctggca atcgcgattg gagtaaagtg
    10621 cctcaccaag cggataatgt aatgttttgc atgattgttg ctgcatatta aaggatgtat
    10681 gaaggaatct tcagaaacat ggataatcca tattatacgt gatttccagg agttgcgttc
    10741 ggtgggggtg actgtattga ccaagaacgt aattgaggcc atcgaggaaa gacgcagcgt
    10801 gagagaattc aagccggacc ctatccccga ctccataatc gcccgcatcc tcgaatgtgg
    10861 acttaaggcg ccaagcgcag ggaatgttca accttggcag ttctgggtgg tcagtcggcc
    10921 tagtctcaag tcagctttgg ccgaagccgc cttcggccaa gaatccgtgg agcaagcgcc
    10981 tgtagtcata gtggttttgg ccgagccttc gcggagcgct gctaggtttg gttaccgcgg
    11041 gtctaagctt tattgcatac aagatactgc agctgcggta cagaacatga tgttggcggc
    11101 aacagcttat ggaatcgggt cctgctgggt aggggctttc gacgagaaga tggtgagcaa
    11161 agcccttggc ctgtccgagc gatttcggcc tatggcattg gtccccatgg gatatccggc
    11221 ggaggtatcg cagcgccggc cgcgttccct gaggccattg gctgagacag tcgtcacggt
    11281 tgagtaagac gctgtcgagc gagcatcgca cgttgaagtg cctggatgcg ctcctactac
    11341 gagagctgtt ttttgcgcag aagcgtgggt taacacgaaa tgcatctgcg cgttaggaca
    11401 aatcacggtt ttggcgcggg ttagcgtggc cgatcagggc ctggagggct aacccgcgct
    11461 tggttttcga tgggaggagt cgatcatgga tccacgattg gaagaacgcc atttccttcg
    11521 accgtcagta gtttatgcta ggggccgagt gccggtggaa gcggcttttg tcgcaaggcc
    11581 caatagattt gtggctatcg ttcgtctcag ctgtgatgtt gagcatgcct acggtctcga
    11641 tcgcgctggc gccgagatca ctgcccacgt agccgatccc ggtcgtcttg aagaactact
    11701 cattcccggg gccaaggtat atgtgttgcc tgctttccct aagggaatgg atacgatgga
    11761 ttactcaccg actctagcag gagtgggaag gaagacactc tatgatcttg ttttggttga
    11821 ctatgaaggt atattggtat ccatagattc acgcgtgccc aatgagctcg tgcacagggc
    11881 tctgcaggca ggcttttttc ctgaacttgc cgagtaccac caaatcgtgc gggaatcggc
    11941 tttcggcagc agcaggtttg atttccatct ttcggctccg ggtcaggtta tagggaccca
    12001 ggctgccatc ttggacgaga acatctctga ttgctttgtg gaagtgaaat ctgtgaccct
    12061 tgttgaggat ggcttggcca aattccccga cgcacccaca gccagaggcg cgaggcacat
    12121 gagggaactc gcccaagcgg tttccgcagg cttgagaggc gtcgtcatct tcgtcataca
    12181 gcgcaacgac gcgtcgaact tcacgcccaa tag
//
LOCUS       DAOSAI010000022        12156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3519_length_12156_cov_3.999339, whole genome shotgun sequence.
ACCESSION   DAOSAI010000022 DAOSAI010000000
VERSION     DAOSAI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3519_length_12156_cov_3.999339"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /gene="smc"
                     /locus_tag="PLM83_01685"
     CDS             complement(<1..2270)
                     /gene="smc"
                     /locus_tag="PLM83_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOK70152.1"
                     /translation="MYLKKLSLFGFKSFASRVDLDFSPGLTGVVGPNGVGKSNIADAL
                     RWALGEQSPRILRGSRMQDVIFAGTESRKPLGYAEVTLVFDNSDDFLNLGFTEVTVTR
                     RLFRSGESEYVLNGVPCRLRDIIDLFSGTGLGKDSYSVVEQSKIDIILSDRSQDRRAI
                     FDEASGIMKYRARRREAERKLVEVRADQLRVGDLVAELGRQLPVLRNQAEEAERWREL
                     TERLTRLEIDLLCHDLKRLSLRRLDSASRIDDLERREHECAAESAQCEAVLESAKTRA
                     MQADLAVETLHVAAGQAAAASERESGRLKLAQERKRTLLERSQEIAAACEAEEGKHAR
                     LENEFALLQGQVDAAGLEGRDVMEQADASRRRLAEASKARADAEARVEEAKSDLFDAL
                     ALESDLRSRIGAIESSKRSNEARLARLIQQAQEKAVAQDSARRETDDLFSELERLRGD
                     LEKAEREAADAKALYERAMEAAESVSERAMEAEAKVSSAAAAHSSIDALQRQYEGYGR
                     AVRALLAGDNGRAMGLAGTVSDLIRVPKEYEIAIEAALGPSVQNIVASTSEGAERAVE
                     FLKKNRAGRATFLPLDILKPSPISSAQLPVAEPGIVGLAADLVESAPIYRKVVEYLLG
                     RVVVVRDLGCGIRLAKSGLRCRMVTLDGDIISAGGAITGGEAPDRRGGLLARSRRLEE
                     LAAELESARAESASIHRLKDEAVAEAGALRLVWEENERNKATLESVLSNVVQKIRLAE
                     AELSRFADELETVELEA"
     gene            complement(2360..2620)
                     /locus_tag="PLM83_01690"
     CDS             complement(2360..2620)
                     /locus_tag="PLM83_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOK70153.1"
                     /translation="MEVLKVSGQSNPKAVAGALAAIVRDQGSVQVNAVGAAAVNQAVK
                     AITIARGFVAPNGIDLVTVPAFSEIEIDGEERTAIKFIVMPR"
     gene            complement(2713..3447)
                     /gene="rnc"
                     /locus_tag="PLM83_01695"
     CDS             complement(2713..3447)
                     /gene="rnc"
                     /locus_tag="PLM83_01695"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOK70154.1"
                     /translation="MCATIEIDKVSAVLGLEFEDKSLLVRALTHTSYAAEHGQDRALS
                     NERLEFLGDAILKAVMAEQLMTLMEDADEGRLSKVSAQAVSGQALARAAVDLGVSDFI
                     MLGHGEESSGGRCRQRNLAGAMEAIIGAVYLDSGFDEARSFIIRALASEIHDAITGPE
                     NDHKTAFQEAVQARSLGQASYRTVKSEGPPHKPWFTVEALIADRVVGTGEGPSKRAAE
                     QEAAKDGLRMLENGESAWVNCASDDE"
     gene            complement(3422..3679)
                     /gene="acpP"
                     /locus_tag="PLM83_01700"
     CDS             complement(3422..3679)
                     /gene="acpP"
                     /locus_tag="PLM83_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOK70155.1"
                     /translation="MTEAEILAKVREIVSDKLGVAEEEICRESDFIDDLGADSLYLNE
                     IVYEIEEEFDLRVPDEDFDSMRTVGQAVDYIYNRVRDDRDR"
     gene            complement(3772..4779)
                     /gene="plsX"
                     /locus_tag="PLM83_01705"
     CDS             complement(3772..4779)
                     /gene="plsX"
                     /locus_tag="PLM83_01705"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOK70156.1"
                     /translation="MRIAVDVMGGDMGPAEMIPGALMARDQYGAQVIFVGDEETIKAH
                     LKENGRDESDIEIVHAADSISMDEDPLAAVRHKRDSSMVVAAKLVREKKAQAAVTSGS
                     TGALVAAGPLVVGRLKGASRPALATPIPTVGGHPCVILDIGANPECQAKNLLEFAIMG
                     SAYASALLGIDNPSIGLLSNGTEASKGTSVIVEANQMLSGSGLNFVGNIEARSIFYGE
                     ADVVVMDGFAGNVVLKLSEGLGSAIFDMVKEEMSKGCMVKAGALLVKPALKSVKRRMD
                     YSEYGGAPLLGLSGLLVKAHGSSNRKAFANAIRVTSELLDRGLVEKMSQSLSAMVDGE
                     A"
     gene            complement(4879..5061)
                     /gene="rpmF"
                     /locus_tag="PLM83_01710"
     CDS             complement(4879..5061)
                     /gene="rpmF"
                     /locus_tag="PLM83_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006697063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOK70157.1"
                     /translation="MPAVPKQKSGKSRTRKRRANWKAEAPTLSVCPQCKKPRLPHRAC
                     PECGYYGGRTVIDVSE"
     gene            complement(5281..5790)
                     /locus_tag="PLM83_01715"
     CDS             complement(5281..5790)
                     /locus_tag="PLM83_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOK70158.1"
                     /translation="MQIDVEGLREEKGSTMSIDMAEDFPSIDLIRSELVFVEPVRFVG
                     QATNTGRRIVVTGIAYGRARATCDRCLSEFELPLESSILETYYRSDEAPDSPDEDERE
                     YGEDNIVDIAPAIEQAFILLLPIRIVCDEECKGLCPQCGHNLNWGDCSCPPDESWGLF
                     QRAKIIHKE"
     gene            complement(5813..7024)
                     /locus_tag="PLM83_01720"
     CDS             complement(5813..7024)
                     /locus_tag="PLM83_01720"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOK70159.1"
                     /translation="MKILVINCGSSSLKYQLFDMDEEKCLARGGVERVGSPDAFMTHT
                     RIGHDKVKRDVDAPDHERALKYVFDALVDPETGVIGSLSDISAIGHRVVHGGERFASS
                     VMIDEEVMKALHECQDLAPLHNPPNIMGIEACRRLMPDLPMVGVFDTAFHQTMPKEAY
                     MYGLPYDMYVNHGVRRYGFHGTSHRYVSQRAAILMKRPIEELSLVTCHLGNGSSIAAV
                     KRGKSIDTSMGFTPLEGLVMGTRSGDLDPAVVSFLCEQLDKSASEIVLGYLNKNSGVL
                     GLSGGLSNDFRDLEKAAAEGHQLARLALDVFAYRVVKYIGAYAAAMGSIDGIVFTAGV
                     GENSSDMRRRICDRLQLFGIELDEKKNSARGVEQIISTPESRVTVMAIPTNEEIMIAR
                     DTAEIVSALGA"
     gene            complement(7127..8011)
                     /locus_tag="PLM83_01725"
     CDS             complement(7127..8011)
                     /locus_tag="PLM83_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOK70160.1"
                     /translation="MWHRFRLILLPLLAAFCVVLLLWACPGANASAVTLRKGDRGENV
                     YELQEQLALAGYLDGEADGVFGNATYAAVIRFQNDVGLTPDGIVGPNTWSSLAQKAQS
                     ASYSKYVVAKGDTMYAIAKKFGVTVEEIAAASGVLRPELIKPGQELKIPICASAASRG
                     TSGRANAELLHWDVAKTIFTARATIIDCATGLSFQVQRRGGHNHADAEPLTAADTAIL
                     KQIYGGKWSWSRRAVIVLVGDRRIAASINGMPHGGCLIKDNNFDGHFCVHFLGSRTHA
                     SALLDPDHQRMVNVAAGL"
     gene            8144..9376
                     /gene="ylbJ"
                     /locus_tag="PLM83_01730"
     CDS             8144..9376
                     /gene="ylbJ"
                     /locus_tag="PLM83_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HOK70161.1"
                     /translation="MKPSLEPNRRNAYLVAALASFLTIAIIALPEQAFQASLDGLRLW
                     WNIVFPALLPFFIASDTLMGLGVVHFIGALLEPLMKPLFGVPGVGAFALAMGLASGYP
                     IGARITARLRREGLCSRVEAERLVSFTNTADPLFMVGAVAVGMFQLPGLGPILAASHY
                     VSAILLGFLLKSYGESDKRRRTEEYQREESLLSRAAHELYTARSQDNRSFGQIFGDAV
                     KDSVGTLLLVGGCIMIFSVVIRVLTVFHITDLLSAPLIAVLAPLGVPQDLVASLVGGF
                     FEITIGTEMASRATASIAQKAIAASAIIAWSGLSVFAQVASMLFGTDIRLTPYFFARI
                     VHAVLAAIVTAIMLKIVPWAAQQTIAIPGLSSSPGFLATVSYSCTMLAKTAAIGASIM
                     AALALLSKPRAFGFRVKK"
     gene            complement(9386..9835)
                     /locus_tag="PLM83_01735"
     CDS             complement(9386..9835)
                     /locus_tag="PLM83_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70162.1"
                     /translation="MIKQSVVECLNRMETIIESSGKLPFSGKRAVDGVELLELIDRIR
                     IALPEEMRQTEALIAERSRLAAEDGVGIASAQASGADGEVFASAREEAEVLLMRAERE
                     AMEIRLGADDYAEATLASLQETLDRTSFVVRKGIEELRRRKDNPEAR"
     gene            complement(9840..10328)
                     /gene="coaD"
                     /locus_tag="PLM83_01740"
     CDS             complement(9840..10328)
                     /gene="coaD"
                     /locus_tag="PLM83_01740"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOK70163.1"
                     /translation="MLTLAVCPGSFDPVTMGHLDIITRAASIFDNVIVAILENQAKDH
                     MFSLEERLEMLRVACAHLPNVQVDHFEGLLVDYVRACGAHVVIRGLRAVSDFEFELTM
                     ALMNRKLDPSIETMYIMTSMEYSFLSSSIVKEVAGSGGDIHGLVPEPLVERVSARCRR
                     KG"
     gene            complement(10329..10898)
                     /gene="rsmD"
                     /locus_tag="PLM83_01745"
     CDS             complement(10329..10898)
                     /gene="rsmD"
                     /locus_tag="PLM83_01745"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOK70164.1"
                     /translation="MRVIGGIAGGRRLATIRGRNIRPTSDRVREAIFNIIADRVEGCV
                     VLDLFAGVGSLGIEAISRGARHCVFVDKDHAACGVIKRNLDDLGFEEASTVVEADAFR
                     AIGAGRGPVSEKAPYDLVFVDPPYAMEASEKLLSLIENARILAGDGLVLVEHSSRDIA
                     PYGSSRMTMVRQERYGDTEVSFYMFSSEG"
     gene            complement(10908..11132)
                     /locus_tag="PLM83_01750"
     CDS             complement(10908..11132)
                     /locus_tag="PLM83_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70165.1"
                     /translation="MSRLAAMTDNSCDASSNTMLDDESCINTVESESFELLKAPRSIR
                     RLVEAWDLRCWSLSRTYWGRELQLRLGPIA"
     gene            complement(11147..>12156)
                     /locus_tag="PLM83_01755"
     CDS             complement(11147..>12156)
                     /locus_tag="PLM83_01755"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70166.1"
                     /translation="LKVAREPMAVKQEIKYAYCTEFIIRGNGLDPEKIKSRLLAEIDG
                     DSMLVVGNENTVKVHMHSNVPGRILEIGIGYGSLHEIDIDNMEDQHEEFEEMSGAENG
                     VFPHSSEKEPYELKDVGIVAVAAGDGVEKILQSLGADVVITGGQTMNPSIQDIVDGIR
                     EAHAKDVIILPNNGNVVLTARQAAEVPDIEARIHVVPSKTIPQGLAALLAMTPYTSVE
                     DNVAKMNEAIGKVVSAEITYAVRDSTFNGIEIRENDIIGIVDGDIQVVGEDVDEVVRK
                     VFTEMYSEEHEIATIIYGFSVTEEEAESLADSLAEEYPDIEFEVQYGGQPLYYYLISI
                     E"
BASE COUNT         2783 a         3762 c         2761 g         2850 t
ORIGIN      
        1 gcttcaagtt ccacagtctc aagctcgtct gcgaaccgag aaagctcagc ctctgccaat
       61 cttattttct gaacgacatt gctcaggacc gactcgagag tcgccttatt gcgttcattc
      121 tcctcccaca ccaacctgag agcgccggct tctgccacag cctcgtcctt gagcctatgt
      181 atactggcgc tctctgccct cgcactctcg agttcggcag caagctcttc aagccgtctg
      241 gaccgcgcaa gcagtccccc gcgcctatca ggtgcctctc cgcccgttat ggcgcctccg
      301 gcggagatga tgtctccgtc caaagtcacc atgcgacacc tcagcccgct tttggcaagc
      361 cggatgccgc atcccaagtc tctcaccact actacgcgtc cgagaaggta ctcaaccacc
      421 tttcggtaga tgggcgccga ttccacaaga tcagccgcaa gcccgacaat tccgggctca
      481 gccacaggaa gctgcgccga agaaatgggg gaaggcttca agatgtccag cggaagaaag
      541 gtagcgcgtc cggcacggtt tttcttgaga aactccacag ctcgctccgc cccttcagag
      601 gtggatgcca ctatgttctg gactgaaggg cccaatgctg cttcaatagc gatctcgtat
      661 tccttcggga cgcgaattag atccgaaaca gtgcccgcaa ggcccatagc ccgcccgtta
      721 tcaccagcca aaagcgcgcg aactgcgcgg ccgtatccct catactgcct ctgtagcgca
      781 tctatgctgg agtgcgcggc agctgccgac gaaaccttcg cctcggcctc catggcacgc
      841 tccgaaacgg attccgcagc ttccatggca cgctcatata atgccttggc gtccgcagcc
      901 tccctctccg ccttttcaag gtcgccccgg agccgttcta attccgagaa aagatcatcg
      961 gtctcacgcc tggcgctatc ctgagctaca gccttctcct gggcttgttg tatcaaacgc
     1021 gccaaccgag cttcgttcga ccgcttcgac gactcaatag cgccaatgcg cgatctcaag
     1081 tcagattcca aagccaaagc gtcaaaaagg tccgattttg cctcctcaac ccttgcttca
     1141 gcgtcagctc gagccttgga cgcctctgcc aaccgtctcc gcgatgcatc agcttgctcc
     1201 atgacatcgc gcccctcaag acctgcggca tcaacctgac cttgcaacag cgcaaattca
     1261 ttttccaacc tggcgtgttt tccctcttcc gcctcgcacg ccgctgcgat ctcctgagaa
     1321 cgttccagga gagttcgctt cctctcctgg gcaagcttga gtctaccgct ttccctctca
     1381 gaagccgcag cagcctgacc tgccgcaaca tgtagcgtct ccacagccaa gtcagcttgc
     1441 attgccctag tttttgccga ctccagcaca gcctcgcatt gggcggactc ggcggcacac
     1501 tcatgttctc gcctttcaag gtcatctatt cgtgacgcac tgtcgaggcg acgaagcgag
     1561 agcctcttga gatcgtggca gagaaggtct atctccagtc gggtaagcct ttcggtaagc
     1621 tcgcgccagc gttctgcctc ctccgcctgg ttccgaagga caggcagctg cctaccgagt
     1681 tcagcaacta gatctccgac cctgagctgg tcagcgcgca cctccaccag ctttcgttca
     1741 gcttcccgcc tccgtgcacg atacttcatg attccagaag cttcgtcgaa aatggcccgt
     1801 ctgtcctgcg agcggtcgga caatatgatg tcgatcttgc tctgctctac tacagagtaa
     1861 gagtcttttc ccagaccagt cccggagaaa agatcgatta tgtccctgag ccgacatgga
     1921 actccgttca atacatactc cgattctccg gatcggaaaa gccttcttgt gaccgtgacc
     1981 tcagtgaacc ccaggttcag aaagtcatct gaattatcga atacaagagt aacctctgca
     2041 tagccaaggg gtttcctgct ttcagttcca gcgaagataa cgtcctgcat tcgcgaccca
     2101 cgaagaatcc gcgggctttg ctcacccaaa gcccatctga gggcatcggc aatattgctc
     2161 tttccaacac cattgggccc gacaactccc gtgagcccag gcgaaaaatc caagtccact
     2221 ctgctcgcaa atgacttgaa gccgaacaga gagagctttt tcagatacaa gttcggccct
     2281 cccatgacgt acactcagca aaagccgcgc caagcggcgc agcctttgcc gattgatctt
     2341 tcaaagttag tattgcccgc taccgcggca tcacgatgaa ctttatagcc gtgcgttcct
     2401 ctccgtctat ttcgatctca gaaaaggcag gcacggtcac caaatcgatt ccgtttggcg
     2461 ccacgaaacc ccttgcaata gttattgcct ttactgcctg gttcacagcc gctgcgccca
     2521 cagcattcac ctgcaccgaa ccttggtccc tgacaatcgc ggccagcgcc ccagcaacag
     2581 cttttgggtt tgactggcca gatactttca aaacctccaa gtcgcactcc ccccgaaacg
     2641 tacatatatg ccgacgcaca agcgccgact gtctccgctt cgccattgtc gccaggcaca
     2701 actggagagt tctcattcat cgtcagaagc gcagttgacc catgcagatt cgccgttctc
     2761 gagcattctc agcccatcct tggctgcttc ctgttctgcg gcgcgtttgc tcggaccttc
     2821 tcccgttcca actaccctgt cggcaatcaa cgcctccacc gtgaaccagg gtttgtgagg
     2881 cgggccttcg ctcttgaccg ttctgtaaga cgcctggccc aaagatctgg cctgcactgc
     2941 ttcttgaaaa gcagtcttgt ggtcgttctc aggaccggta atagcatcat ggatttcgct
     3001 tgccagtgcc cgaatgatga aagatctggc ctcgtcgaag ccggagtcga gatatacggc
     3061 gccaataatg gcctccatag cgcctgccaa gtttctctgt cgacacctgc cgccgctcga
     3121 ttcttcacca tgccccaaca tgataaaatc ggagactcca agatcgacgg cggcccgtgc
     3181 caaggcctgc ccgctcactg cctgagcaga taccttcgac aacctgccct cgtctgcatc
     3241 ttccatgaga gtcatcagct gctccgccat aacggctttg agaatcgcat cgccaagaaa
     3301 ctcaaggcgc tcgtttgata aagcccgatc ctggccatgc tctgctgcat aagacgtatg
     3361 ggtcaacgcc ctaactagaa gagatttgtc ttcaaactcg aggccgagaa cagccgaaac
     3421 cttatcgatc tcgatcgtcg cgcacacggt tgtatatata gtcgacagcc tgacctacag
     3481 ttctcatgga atcgaagtct tcgtcgggca cacgcaggtc gaattcctct tcaatttcgt
     3541 acacgatttc gttgaggtac agcgagtcgg cgccaagatc gtcaatgaaa tccgactcgc
     3601 gacagatctc ctcctctgca acaccaagct tgtcggagac gatctcacga actttagcaa
     3661 gtatttcagc ttcagtcaaa actgaccacc tccatgcaag gatattcaat cccatcttat
     3721 aaagcaatta ccttcatttc aagcccgtac ccgataccat tgcgtccagt ctcaagcctc
     3781 gccgtcaacc atggccgaaa gagactggga catcttctca acaagtcctc tatcaagcag
     3841 ttcggaagtg actctgatag cgttggcaaa cgccttacga ttggagcttc catgggcctt
     3901 gacaagcaga ccggacaggc ccaagagagg agcgccccca tattctgaat aatccatcct
     3961 cctcttgaca ctctttagcg caggtttcac gagaagagct cctgctttca ccatgcagcc
     4021 cttgctcatc tcctctttca ccatgtcaaa aatggctgat ccgagaccct cagaaagctt
     4081 gaggacgaca ttaccagcaa aaccgtccat tacaacgacg tcagcttctc catagaagat
     4141 cgaacgggct tcgatattgc ccacgaagtt cagccccgac ccggacagca tctgattcgc
     4201 ctcgacaatc accgaagttc ccttgctagc ttcagtgccg ttcgacagca aaccgatgct
     4261 gggattatct attccgagaa gcgccgaagc ataagctgat cccataatcg cgaactccaa
     4321 cagattcttc gcttgacact ccggattagc gccgatgtca aggataacgc aaggatgacc
     4381 tccaacggtc gggataggcg ttgccagagc tggcctcgat gcccccttaa gccgccctac
     4441 aacgagaggc ccggccgcaa cgagagcccc tgtgctgcca gaagtgaccg ctgcttgcgc
     4501 ctttttctca cggacaagct tagctgctac gaccattgag gagtcgcgct tgtggcgcac
     4561 agccgcaaga gggtcctcgt ccatggatat cgagtctgcc gcgtgcacga tctctatgtc
     4621 agattcatcc cgtccgttct ccttcaggtg cgcctttatc gtctcctcgt cgcccacaaa
     4681 aatcacttgg gccccatact gatcacgggc catcaacgct cccgggatca tctcagccgg
     4741 ccccatgtcg cctcccatga cgtcaacagc tatccgcata tggataaccc cccaaaaaag
     4801 ctcaacaaaa gctgaaagcg ggttcagccg aactacgcgc gtccgaatga atccgctctg
     4861 cacccttagg ttgaagaatt actctgaaac atcaataact gtgcgacccc cgtaatatcc
     4921 gcactccggg catgccctat ggggcagcct gggcttcttg cactgtgggc aaacggagag
     4981 ggtcggggcc tcagctttcc agtttgcgcg acgcttacga gtgcgcgact tgccggattt
     5041 ctgttttgga acagcaggca tattcttggt ttcgtcgagg cacaaaccgc gcgccccgag
     5101 gaaacctctc cacctccatt caatcatgat taagctatga tattgtacta ctcttggacg
     5161 aagcaggcaa gttttcttcg ccaggtctcc atactctccg tgccgttcga gggcaggaga
     5221 gtatcgccaa ccgtgtcagc atcaaagccc taccacatcc agaacgaaac agtgttgttt
     5281 tcactctttg tggataatct ttgccctctg aaacaagccc cagctctcat cgggagggca
     5341 cgaacaatcg ccccagttca agttgtggcc acattgcggg cacaacccct tgcattcttc
     5401 gtcacacact atcctgattg gaagcaaaag aatgaaggct tgctcgattg ccggggctat
     5461 atcgacaata ttatcctccc catactcccg ctcatcttcg tcgggggaat caggggcctc
     5521 gtcggatctg tagtaagtct ccagaatgga cgattccaaa ggcaactcaa attccgatag
     5581 gcatctgtca cacgttgcac gtgcgcgacc gtatgctatc ccagttacaa cgattcggcg
     5641 tcccgtatta gtggcttgcc ccacaaatct gaccggttca acaaacacga gctccgagcg
     5701 aatcagatca atcgaaggga aatcctccgc catgtcaatc gacatagtgg accccttttc
     5761 ctccctgagc ccctcaacat ctatctgcac ctcaatacca cccctgccgg gatcaagctc
     5821 ccagcgcgct aacgatctcc gcagtgtcgc gcgcgatcat gatctcctcg ttggtaggta
     5881 tagccatgac agtcacgcga gactcgggcg ttgatatgat ctgctccaca ccccttgcac
     5941 tattcttttt ctcgtccagt tcaatcccga acagctgcag ccgatcacat attcttctgc
     6001 gcatgtccga cgaattctcc cccaccccag cagtgaatac tattccatca atgctgccca
     6061 tagcagcagc gtaagctcca atgtatttta ccaccctata agcaaacaca tccagggcca
     6121 gtctggccaa ttgatgccct tccgcagcgg ccttctcaag atcccggaaa tcgttggata
     6181 gccctcccga aaggcccagc actcctgagt ttttgttgag ataccccaac actatctcgc
     6241 tcgcgctctt gtccagttgc tcacagagga aagatacgac ggcagggtcc agatcgccag
     6301 atctcgttcc catgaccagc ccttccaagg gagtaaaacc catgcttgta tcgatggact
     6361 ttccccgttt caccgctgct atgctcgatc cattcccaag atggcatgtc accagggaga
     6421 gttcttctat aggccttttc atgaggatag cggcacgttg tgagacgtat ctatgtgaag
     6481 tgccgtggaa accgtaacgc ctcactccat ggttcacata catgtcatat ggtaagccat
     6541 acatataggc ctctttcggc atggtctgat ggaaggcagt atcaaagact cctaccattg
     6601 gcaaatcagg catgagccta cggcaagcct caattcccat tatgttaggc ggattatgca
     6661 gaggtgccag gtcctggcat tcatgcaaag ctttcatgac ttcttcatct atcatgaccg
     6721 aactggcaaa cctttcgcca ccatggacta ccctgtgtcc aatcgcagag atatcagaca
     6781 ggctgcctat tactccagtc tccggatcta caagggcgtc aaatacgtat ttcaacgcgc
     6841 gctcatgatc cggagcatct acatcacgct ttaccttgtc atgtccgatc cgtgtatggg
     6901 tcatgaacgc atccggcgac ccgacccgct ccacgcctcc acgagcaaga cacttctcct
     6961 catccatgtc gaacagctga tacttcaatg atgaacttcc acagttgatt accaggatct
     7021 tcattacacc gatgacctcc taacctttga aaaagaaaac atggcataca ccatgttagg
     7081 caggcacgcc cctgacgagt tgacgcgagc ataaaggggc tctggcctac aaaccggccg
     7141 caacattgac catcctctga tgatcaggat ccagcaaagc gctggcatgg gttcgactgc
     7201 ccaagaaatg aacacagaag tggccatcga agttgttgtc cttgataagg caacctccgt
     7261 gcggcatccc attgatcgaa gcggcaattc gtctgtcccc gactaatacg ataacagctc
     7321 ttctggacca actccatttt ccgccgtata tctgcttcag tatcgcagta tcagccgccg
     7381 tcaacggttc agcgtcagcg tgattatgtc ctccacgcct ctgtacctga aacgagagcc
     7441 ctgtagcaca atcaatgata gtagcccggg cggtaaatat agtcttcgcc acatcccaat
     7501 ggagaagttc ggcgttggct ctgcccgagg tgccgcgtga ggctgcagac gcgcaaattg
     7561 ggatcttcag ttcctggccg ggtttgatca gttcaggccg caagacccca gaggccgccg
     7621 ctatctcttc cacagtcact ccgaatttct tggcaatagc atacattgtg tcgcccttgg
     7681 ccaccacata cttgctgtat gatgccgact gtgccttctg tgcaagagag ctccatgtgt
     7741 tagggccgac gatgccgtct ggcgtcagac caacatcatt ctgaaaacga atcactgcgg
     7801 cataggtggc gttgccaaaa accccatcgg cttcaccgtc gagatagcct gcaagcgcca
     7861 gttgctcttg gagttcgtac acgttttcgc ctcgatctcc ctttcgcagc gtgacagcag
     7921 aagcgtttgc ccccggacat gcccaaagaa gcaatacaac gcagaacgcg gccaacagag
     7981 gcagcaaaat caagcgaaac cgatgccaca cccttctcgc ctcccaaaat aacgccttac
     8041 ccgtatttct accgaacgcc gtaaaatcct tcagcatatg caggagctct ggcaaatata
     8101 gatctgagga cgacccacag cacgaaagga agtgtatgct cctatgaagc cgagcttgga
     8161 accgaaccgg cgaaatgcat atctcgtcgc cgcattggcc tcattcctca ctatcgctat
     8221 aatcgctctc cctgaacaag cgtttcaagc ttccctagat ggcctacgcc tctggtggaa
     8281 catagtgttc cctgcccttc ttccattttt catcgcttca gacaccttga tgggcctcgg
     8341 cgtcgtgcac ttcatcgggg cactcctaga gccgctcatg aagcccctat tcggcgttcc
     8401 tggagtagga gcattcgcct tggccatggg gctcgcatcc gggtatccta taggcgcacg
     8461 cataacagca aggctccgaa gagaaggtct gtgttccagg gttgaagccg aacgccttgt
     8521 ctctttcacc aataccgctg atccgctttt catggtgggc gccgtggccg tgggaatgtt
     8581 ccaacttccc ggtcttggcc cgattctcgc ggcttctcac tatgtttcag ctatactcct
     8641 tggctttctg cttaagtcct atggagagag cgataagagg agaaggacag aagaatacca
     8701 gagggaagag tcgctgctct cccgtgcagc ccatgaactg tacactgcta ggagtcaaga
     8761 caacagatca ttcgggcaaa tcttcggaga cgcagtgaag gactccgtag gaacgctgct
     8821 tctcgttggc gggtgcataa tgatattttc cgttgtgatt cgcgttctca cggtgtttca
     8881 catcacagac ttgctctccg cgccactcat agctgtgctt gctccactcg gcgtcccgca
     8941 ggatttggtg gcttcactcg taggcggttt ctttgagatt acaataggca cagaaatggc
     9001 aagccgcgcc accgccagca tcgcccagaa ggcaatagcg gcgagcgcaa taatcgcgtg
     9061 gagcgggctt tccgtatttg ctcaggttgc gagcatgctc tttggcactg atattcgact
     9121 cacgccatac ttcttcgcca gaatagtgca tgcagtactt gctgccatag tgacagctat
     9181 catgctcaag atagttccat gggccgccca gcagacaata gccattcccg gcctctccag
     9241 ctcgcccggt ttcttggcca cagtcagcta ctcctgcaca atgcttgcca aaaccgcagc
     9301 cataggggca tccattatgg cggcgctagc gcttctttcc aagccccggg cattcggctt
     9361 cagagtgaaa aaataggcaa tcgaattacc tggcctcggg attgtccttc ctgcgcctca
     9421 gctcttctat gccttttctt actacaaacg atgttctatc cagagtttct tgaagagaag
     9481 cgagggtagc ctcggcataa tcgtccgctc caagcctgat ctccatagct tcgcgttccg
     9541 cacgcatcaa cagcacctcc gcctcctccc tcgcgctggc aaagacctct ccatcggcgc
     9601 cagaagcctg tgctgatgcg atcccaacgc catcctcggc agccaaccgg cttctctcgg
     9661 ctatcagggc ctctgtctgc ctcatctctt caggcagcgc aatccgtatc ctgtcgataa
     9721 gctcgagaag ctctactccg tcaacagcac gttttcccga aaaaggcagc tttccgctgg
     9781 attcgattat cgtctccatc cgattcaggc attcaacaac cgattgtttg atcataatcc
     9841 taccccttcc tcctacacct ggccgacacg cgttccacca atggctcagg caccaggccg
     9901 tgaatgtctc ctccagagcc tgctacttct ttgacaatgc ttgagctgag gaatgagtat
     9961 tccatagatg tcattatgta catggtttct atggacggat cgagttttct gttcatcaaa
    10021 gccatagtca gctcgaattc gaaatccgac actgcgcgaa ggcctcttat gacgacatgt
    10081 gcaccacaag cccgcacgta gtcgaccaaa agcccttcga aatgatcaac ctgcacattg
    10141 gggagatggg cacaagcaac cctcaacatc tccaaccttt cttccaggga gaacatatgg
    10201 tctttagcct ggttttccag gattgcgaca ataacattat caaagatcga tgctgcacgc
    10261 gttataatgt caaggtgtcc catagttacc ggatcgaaac tgcctgggca tactgcaagg
    10321 gtcaacagct atccctccga cgagaacata tagaaactga cttcggtatc tccgtagcgc
    10381 tcctgcctca ccattgtcat ccttgaggat ccatatggag cgatatcccg tgatgagtgt
    10441 tctacgagaa ctaggccatc tcctgcgaga attcgcgcat tctcgataag ggacaacaac
    10501 ttttcagaag cctccatagc atacggtgga tccacgaata ccagatcata tggcgccttc
    10561 tctgatacag gtcccctacc cgcaccgata gcgcggaacg cgtcggcctc tacaactgtc
    10621 gacgcctcct caaagcccaa gtcatccaga tttcttttta tgacgccaca agcagcatgg
    10681 tccttgtcaa caaacacgca gtgccttgct ccgcgcgata tcgcctcaat accaaggctt
    10741 cccacgcccg cgaaaagatc gagtacgaca cagccctcaa cgcgatcggc aataatattg
    10801 aagatcgcct cgcgcacacg gtcagaggta ggtctaatgt tgcgccccct gatcgtagcc
    10861 aaccggcgac ctcctgcaat gcctccaata acacgcatct tacttgacta tgctattggg
    10921 ccgagcctca gctgcagctc ccgaccccaa tacgttctcg acaatgacca gcaccgtaaa
    10981 tcccacgcct cgacaagcct acgaatggac ctaggcgcct tgagcaactc gaagctctct
    11041 gattccaccg tattaatgca gctttcatcg tccaacatgg tgttggagga agcatcgcaa
    11101 ctgttgtccg tcattgcagc aagccgagac atggctcatc cccctttcac tcaatagaga
    11161 taaggtaata gtaaagcggt tgaccgccgt attgcacttc gaactcgatg tcagggtatt
    11221 cctctgccaa gctatccgcg agcgactccg cttcttcctc agtaacactg aacccgtaga
    11281 tgatagtggc gatttcgtgt tcttcagagt acatttccgt gaaaaccttg cggacgactt
    11341 cgtccacgtc ttcgccaaca acctgaatgt caccgtcaac tatgccaatt atgtcgtttt
    11401 cgcggatctc tattccattg aacgtagagt cacgcactgc atacgtgatc tcggcgctca
    11461 ctaccttgcc gatagcctcg ttcatcttcg ccacattgtc ttccacagaa gtatacggcg
    11521 tcatggctaa gagcgcagct aagccttggg gtattgtctt gctcggcacc acgtgtattc
    11581 tcgcttcgat atcaggcacc tcggcagctt gtcgggcagt gagaaccaca ttcccgttat
    11641 taggaagaat gatgacgtct ttggcatggg cttcgcgtat cccatcaacg atatcctgaa
    11701 tgcttgggtt cattgtctgc ccaccggtta tcacaacatc agcgccaagg ctctgcagta
    11761 tcttctctac accatccccg gccgccacgg caacgatgcc cacatccttc agctcgtagg
    11821 gctctttttc gctggaatgc gggaacactc cgttttccgc accgctcatt tcctcgaact
    11881 cttcgtgctg gtcctccatg ttatctatgt ctatttcgtg aagcgagcca tacccaatgc
    11941 cgatctcgag aatccgtcca ggcacattgc tgtgcatatg caccttaact gtgttttcat
    12001 tcccgacgac caacatggaa tctccgtcaa tttcggcaag aagccggctc tttatcttct
    12061 caggatcgag gccgtttcca cgaattatga actcagtaca gtaggcatac ttgatctctt
    12121 gcttcacagc cataggctct ctggcaactt tcaacg
//
LOCUS       DAOSAI010000023        12038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3554_length_12038_cov_4.168238, whole genome shotgun sequence.
ACCESSION   DAOSAI010000023 DAOSAI010000000
VERSION     DAOSAI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3554_length_12038_cov_4.168238"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..838
                     /locus_tag="PLM83_01760"
     CDS             113..838
                     /locus_tag="PLM83_01760"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOK70167.1"
                     /translation="MLAQNYLKSVQQTLDQVEKTQLEAIGRAADAIAEAIGNGGRLHI
                     FGTGHSHIIAEEAFYRAGGLVPVNAILEPALMLHDGPFKSTDMERLEGYAEIILDHSG
                     IRPGEVLMIVSNSGRNAVPVEMALTAKARGIIVVALTSLAHSKSVPSRHSSGERLFDV
                     ADIVIDNCGVVGDAVLPVNGLPTNVCPTSTVVGAAIINMIQAEVVERLGAMGIVPPVF
                     VSANVDGGDEIDRQWRGAFEKRA"
     gene            950..2212
                     /locus_tag="PLM83_01765"
     CDS             950..2212
                     /locus_tag="PLM83_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK70168.1"
                     /translation="MKRVVTAIVVGLVLAAMIVPVSAAKKVTINFWQAGGDSQTAPVM
                     RQIIKEFEAANPDISVNFQAVPWGEDPHVKFQAAIVGGTIADVFTIGDPFQHVLAASG
                     ALEPLDKYMSKEMKRDFYPQFLERCSVDGKVVALPWFGDVRAMLYRKDLFKAAGVPEP
                     NPSWTWDEFLTYAKKLTRDTDGDGVIDQYGFGTSGRYVSQYQVFLVQNGVNFIDEEKG
                     IATANTPAAIEAMQFYVDLVRKHKVTPPGITTIALQDIQKMFAEGKVAMFFDASDTAV
                     RFSKEPALAGKLGVALLPHNKAHGAFGGSDVIVMSSQSRNKAAAWKFLSFMVSREPML
                     AYCKACGFSPVLKSASTDPYVKADPIRKVYTEQMEIGGFFYFKHPKASSMTPVIRAEV
                     QQAMEGTKSVQAAMNDMQKALEDLLKSK"
     gene            2305..3225
                     /locus_tag="PLM83_01770"
     CDS             2305..3225
                     /locus_tag="PLM83_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK70169.1"
                     /translation="MKSRPVAEKKRVLYRLGEVLESYTMLAPAYVIFLVFIFVPVVWA
                     FYLSFFDYSILSMRTPEFSGIDNFRRVFSDPVFWTALKNTVRYSLGTVPTRLVLGLLL
                     ALALDQANLWGRKVFRTIYFLPVVTSMVAAAIVWSLVFSASNNGLANQFLSLFGVAPK
                     GWLSDSNLAMFSVCVLSVWKDLGYVMTIFTAGLQGIPLELYEAGAIDGATVWQRIRFI
                     TIPLLRPTTFFILVTEIISSFQVFTQTYVMTSGGPGYSTTTLINLLHNKGFVQFDMGY
                     ASALAVALFISLLFLSWVLRKVFKADEIVY"
     gene            3255..4121
                     /locus_tag="PLM83_01775"
     CDS             3255..4121
                     /locus_tag="PLM83_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK70170.1"
                     /translation="MTPKLKKKRTCREIVLAGLKYILLVAGAIVMVAPVLWMLSTSLT
                     PDSVIISYQLIPSKITFENYVRAWDFPRVFSDSVTLGTFFFNSVLVSALITGPALIID
                     SLAGYVLARRDIPGKNLLFYLALATMMIPFYVIAVPLFLVVRKLRWLDTFQGMIVPFL
                     SSGFGVFMFKQFFQSVPKELEDAALVDGCSPWRIYWSIMLPLAKPVVGTMTIFKAMWS
                     WNQFFWPLLIINNIKLKTLPLALTMFRGLNVTEWGTLCAGMTIATIPIVVVYLLMQDS
                     FQKGIVMGAVKG"
     gene            4146..5825
                     /locus_tag="PLM83_01780"
     CDS             4146..5825
                     /locus_tag="PLM83_01780"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HOK70171.1"
                     /translation="MRRTGACRADELLLSMGLREKIGQMTIVGFPERVAGELPEGIES
                     LLKDPGVGGVGIFARNARGPAEMLELTSALQDAAIRAGAKVPLFIMADQEGGMVMQVT
                     DGTTVFPGNMALAATSDAENAHEACRAIALESLAMGINVVTAPDVDVNADPDNPIIGV
                     RAYGDDPALVGRFGSAAVRGYEEGAVLCMAKHFPGHGRTAVDSHLAVPLVDSPIEEVE
                     ALDLPPFEEAVRSGVRAVMTAHIQVPALDTTGACGTLSRPILTELLRGRMGFRGLIVT
                     DCLEMGAIKDTIGTPEAAVRAVEAGADMVLICHTLELQRQAIDGMEQAVLDGRIPREA
                     IDASCRRIITTKLELEEARLRMWTDGRPPLDVVGCRDHLEAEQRIARQSITVLRNDDG
                     VLPFRGDGPKTIAVIYPATMPRLRAEDLAEGDTVLGKEFASRGVTSVEVGVSLCPTRD
                     EIDAALAAAGRADAVVIATSCKTREQEKAQGELVQMLNGAVSAPVVACGIRNPYDIRA
                     YPEVRTYLVTYGYRPCSIRAMVEVMFGEIPAKGRLPVEIPGFYPRGYGLEI"
     gene            5986..7140
                     /locus_tag="PLM83_01785"
     CDS             5986..7140
                     /locus_tag="PLM83_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOK70172.1"
                     /translation="MSTNGRLVLYCVIGCLMLACCAAPVAAKDRKTLAVWMWGSTVRA
                     QGAEAVVKSLAANNVTDVILLVRGTAGKAEYVSAIAPPAEEGKDALGDIIAVCRPLGI
                     RVHAWFVFNQDKSYTDLHPEDRIWHHGKANTDFKPYPVDDGKVCPISQSHLDYTNDMI
                     REVIENYDVDGIHLDYIRYGHAVYCFCPDHVQKATALGINVDRVREVMFETFYASNAN
                     PNRFFEAYESGDADVVAWVNMRQAEIRDAAASMRAAAKLAKPDVIFSASLMPEGAFPE
                     TNSFAMCHYGQSYEDAGELYDFVCPMAYHLDYGKTPAWVGDVARGAAKLVGDDVPVYI
                     GVQAYGEKADRDAVQKAIEAGLGAGAEGVALFRFGTMNEERWSAVTDALK"
     gene            7176..8249
                     /locus_tag="PLM83_01790"
     CDS             7176..8249
                     /locus_tag="PLM83_01790"
                     /inference="COORDINATES: protein motif:HMM:NF021354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HOK70173.1"
                     /translation="MPHPVAMGTVMAEMNVKTFSHTVVGLALIVVMGLVGVKATAQTA
                     EGEGILGLFVPVGTISDGSSAYAEQLRAYRLVNMLMRLGLPAFWIAEPFEYEDDMLPR
                     GSFWVPAPDGTPSVAALAQELADRLGVPYTIVYARRFASGEPRISAFRLRPVSVAVYQ
                     GDRTYSGAADIASSLELVGVYPRYVTDIEIRRGALDDYDILVMPGGAQDVQSYALGPA
                     GKANIRSFVESGGGYLGICAGAYLASAPQFELNLVDAPSQGRGVIGTWIAVRQRPGSD
                     PLFRGYDDMISDLYYGGGPNFVSDHRAIAVLEGVPQSWKPGLQGSAAVIEDTCGIGCA
                     ALVESSSRHCCSRMHIRPGVIAR"
     gene            8182..8919
                     /locus_tag="PLM83_01795"
     CDS             8182..8919
                     /locus_tag="PLM83_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70174.1"
                     /translation="MSNPPHATVVRACISDLESSLDEISYCIGQTCNITARLGDKTER
                     SKFFWNYCYFGWTMKEPARRIRELPAKLSRLADDYEQLEQVSATFEGEAFGFPGYSAG
                     ECMLRSWNAVEAVSRDAAVLQPLWRRISEDMAEIECDVDDLLAKEKAGATPTELDWWR
                     LNIREYLVAIELMGGLNWKYWVSGDSTEEPVRAGYDDEYGDPTQTVAASTTRGAIGTL
                     TMGAMRLDEVLDANLAALAIAEMMLGI"
     gene            8964..10037
                     /locus_tag="PLM83_01800"
     CDS             8964..10037
                     /locus_tag="PLM83_01800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK70175.1"
                     /translation="MDGPRPPLEQELTEVVELVNTVFSPEDRTMGLEFPQLFSGGNLD
                     RLRVFVDQTAGIPVAHGGYVAQELVVEGWRIPVAALGSVCTLPAYRGKGLASRLVESI
                     MDQARSEGLVMMLISGDSNVYKRLGAVETGDFVRVEAHQDQLSYLCEIARAWGDDREI
                     VCRQAGAEDVPSMAATYRREPVRYARSVKDFHELAWWRSDVKRLYAYESIQVACTGRE
                     TLAYIVTRVRTLDGGGVRLHVPEFAGPRWAVACLVGHACTAFSPNVLTMTVCSSDRDM
                     VETLRRAALPLRRTTLPGYTVMALDSSALLSSAGSIISERGGAARDDSLSRLLAISGA
                     DLVKTLFGGTDAIPLPVPGLNFI"
     gene            10052..11056
                     /locus_tag="PLM83_01805"
     CDS             10052..11056
                     /locus_tag="PLM83_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOK70176.1"
                     /translation="MDVVIGADGGGTKTACAIAQMDGNIIGLGYAGPSNHLRLPGSHA
                     QAAETVLEGVQAAFSDAGIAVIRARQAYLALGGVDVDRPNEKMAEAARQVLDAEEIAT
                     DNDAAAGLAAATGELYGVVIIAGTGSIAMAVDGEGRRRRSGGWGYRIGDEGSGQWIGR
                     LGLEAAAKAHDGRGKPTSLADRVLDHFGIETVDGLRSVLYKQPVNNRDIADFCMQVMA
                     SAREGDEVALDIVNRAGRELALAVWSAADGLGMASAEFPVVRQGSVFKAGDVIARPFE
                     EELGRLAPAATVVSAAFPPVVGAVMLALMRAGVEIDGAVRENLARSWEEVEARHERLS
                     "
     gene            11040..>12038
                     /locus_tag="PLM83_01810"
     CDS             11040..>12038
                     /locus_tag="PLM83_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOK70177.1"
                     /translation="MSVYLEAAVDMKDEIEEIWTALHGMPEPPFQETKTAGFIAYRLS
                     EAGFDVKKGLADTGIVADLGDPAAGDLTGPVIALRADMDAMRHDIDGREVWVHSCGHD
                     AHSTIVITAGLMLARRCAGARGRLRLIFQPGEETGLGAGRMIEAGAVRGVDALLGMHL
                     RPVSECRVGQATPCLWHSAGRTLSYSVVGKPSHTGRPHLGINVADAVAMATLAVNNVK
                     PDPLRCATVNVVALSAGDGPGGVIPERGSLTVNIRSESDQIADELSSKVDGAVRAVVA
                     ANGAEAGLKREKQMPAPRYDEGMIALARRAIVSVLGEGGVVEKITTPGSEDFHWYAR"
BASE COUNT         2605 a         2953 c         3770 g         2710 t
ORIGIN      
        1 gggcggggcc ggacgggtac gagcagctga tgtgcgatct tgctcctgaa gcgacagaag
       61 agatactaac ggcagtggcc agactttcga gcaaagggat gtgcgtcgac caatgttagc
      121 ccaaaactac ttaaaaagcg ttcaacagac cctggatcag gtagaaaaga cacaactcga
      181 ggcgataggc agagccgctg atgcaatagc cgaggcaatt ggaaatggcg gaagactgca
      241 tatcttcgga actggccact cccacatcat tgcggaagag gcgttttaca gggcgggcgg
      301 gcttgtgccg gtcaacgcaa tactcgaacc ggcgttgatg cttcacgatg gtccgtttaa
      361 gtccacagac atggaacggt tggagggata cgcggagatc atcctggatc actccggcat
      421 tcgccctggc gaggtcttga tgatcgtatc aaattcaggc cggaacgctg ttccggtgga
      481 gatggctctt actgctaagg cgcgcgggat tattgtagtg gctcttacat cccttgcgca
      541 ctcgaagagc gttccctccc gccattcaag cggagagcgc ctttttgacg ttgccgatat
      601 tgttatagac aactgtggtg ttgtaggcga tgccgtgctg ccagtgaacg gtctgcccac
      661 gaatgtctgc cccacatcca ctgtggtggg agctgcaatc atcaacatga ttcaggcgga
      721 agtagtggaa cgcctaggcg ccatgggcat cgtgcccccg gtgtttgtaa gcgccaacgt
      781 ggatgggggc gatgagatcg accggcagtg gcggggggcc tttgagaaaa gggcttgagc
      841 aacaattttg aggttaggag gtggatgatg gcaataggct ttgcacacgg cagttcttcc
      901 gatgcgcgat ttagtcgttt ccataaaaaa tgatcaggag gcttgaacaa tgaagcgggt
      961 tgttactgcc attgtagtag gtctagttct tgcggccatg atcgttcccg tgagcgcggc
     1021 gaagaaagtc acaatcaact tctggcaagc tggcggagac tcccaaacag ccccggtgat
     1081 gaggcagatc atcaaggagt ttgaggctgc gaaccccgac atcagtgtca acttccaggc
     1141 tgtgccgtgg ggagaggatc cacacgtcaa gttccaggca gccatagttg gtggaactat
     1201 tgctgatgtg ttcacgatcg gagatccctt ccagcatgtg ctggctgcaa gcggcgcctt
     1261 ggagcctttg gacaaataca tgtcgaagga aatgaaacga gacttctacc ctcagtttct
     1321 ggagagatgc tcggttgatg gcaaggtagt ggcactgccg tggtttggcg atgtccgtgc
     1381 catgctctac cgcaaagacc tattcaaggc ggccggcgta cccgaaccca atccatcatg
     1441 gacatgggat gagttcctaa cctatgcaaa gaagctcact cgcgacacag acggtgatgg
     1501 cgttatcgac cagtatggat tcggcacgtc aggccgttat gtctcgcagt accaggtgtt
     1561 cttggttcag aacggagtca acttcataga tgaagagaag ggcattgcta ccgcaaacac
     1621 ccctgcggca atcgaggcaa tgcagttcta cgtggatctc gtgagaaagc acaaggtgac
     1681 ccctccgggc atcaccacaa tagcgttgca ggatattcaa aagatgttcg cagagggcaa
     1741 ggttgcgatg ttcttcgacg cgtctgacac ggcagtcagg ttctcaaaag agccggcgct
     1801 cgctggcaag ctcggtgtgg cattgcttcc ccataacaag gcacatgggg cgttcggcgg
     1861 ttctgacgtg attgttatgt ccagtcagtc caggaacaag gcagctgcgt ggaaattcct
     1921 atcattcatg gtatcccgag aaccaatgct cgcgtactgc aaggcatgcg ggttctcgcc
     1981 tgtgctcaag tcggcctcta cagatccgta tgtaaaggct gacccaattc gaaaggtata
     2041 cacagagcag atggagatag gtggcttctt ctacttcaaa caccccaagg cctccagcat
     2101 gactcctgtg atcagggctg aagtgcagca ggctatggaa ggcactaagt cggtgcaggc
     2161 agcaatgaat gacatgcaaa aagcgcttga ggacttgctg aagagcaagt agaggctact
     2221 acggttgtcc tccgccggac ccacgttgac cgtggtgtcg ggtccggcgg aggacaggac
     2281 tccgggagag gggagacggt gactatgaaa tctaggcctg tggctgagaa aaagcgtgtg
     2341 ttgtatcgac tcggggaagt cctggagtcc tatacgatgt tggcgccggc ctacgtgata
     2401 ttcctggtat tcatattcgt ccccgttgta tgggcgttct atctcagttt tttcgactat
     2461 agcatcttgt cgatgaggac ccctgagttt tcagggattg acaacttcag acgggttttc
     2521 tccgatccgg tattctggac tgcgctcaaa aacaccgtaa ggtactcgct ggggacggtg
     2581 cccacgcgtc ttgtcctcgg tctgcttctt gctctcgcgc tagatcaagc aaatctatgg
     2641 ggacgaaagg ttttccgaac catctacttt cttcctgttg taacatcaat ggtcgccgcc
     2701 gcaatcgtct ggtcgctggt attcagcgca agcaacaacg gtcttgccaa tcagtttctc
     2761 agcctcttcg gcgtggcgcc caaagggtgg ctctctgatt cgaatctcgc catgttctca
     2821 gtttgcgtgc tgagcgtgtg gaaggacctt ggatatgtga tgaccatatt cacagcaggt
     2881 ttgcagggca ttcccctgga gctctacgag gcgggggcaa ttgacggcgc gacggtatgg
     2941 cagagaataa ggtttatcac tatacctcta ttgaggccca ctacgttctt catacttgtc
     3001 accgagatta tcagctcgtt ccaggtattc acgcagactt atgtcatgac ttcaggcggg
     3061 ccgggatatt cgactaccac tctaatcaac ctgcttcaca acaagggctt tgttcagttt
     3121 gacatgggat atgcaagcgc attggctgtg gcattgttca tttcattgct cttcctgagc
     3181 tgggtgctta gaaaagtgtt taaggcagac gagatcgtat actaggcttc aaagtcgaac
     3241 ggagggcgat gaacatgact ccaaagctaa aaaagaagcg cacgtgcagg gagatagttc
     3301 tggcaggact caagtatata ctgcttgtag caggcgccat tgtgatggta gctcctgtgc
     3361 tctggatgct gtcaacctcg ctaactccag actcggtcat tatctcttac cagcttattc
     3421 cttcgaaaat cacatttgaa aactacgttc gcgcctggga tttcccccgg gttttcagcg
     3481 actctgtgac tctgggaaca ttctttttca acagtgtgct tgtgagtgcc ctaattactg
     3541 gtcctgcatt gattatagac tcgttggccg gatatgttct ggcgcgaagg gacattcccg
     3601 gcaagaacct cctattctac ctagccctgg ccacgatgat gattcccttc tacgtcattg
     3661 ccgtgcctct cttccttgtt gtgaggaaac tacgttggct cgacacattt caaggcatga
     3721 tcgtaccgtt tctttcgtcg ggcttcggag tattcatgtt caaacagttc ttccagtcag
     3781 tgccaaagga gcttgaggat gcggcacttg tggacggctg ttcgccatgg agaatctact
     3841 ggtcaatcat gctgccgctg gcgaagccgg tagtcggaac gatgaccatc ttcaaggcca
     3901 tgtggtcgtg gaaccagttc ttctggcccc ttctcattat caacaacatc aagctgaaga
     3961 ctttgccgtt ggcgctcacc atgttccggg gtttgaacgt aacagaatgg ggcacgttgt
     4021 gtgccggcat gacaattgct acgatcccaa tagtcgttgt atacctgctc atgcaggact
     4081 cgttccagaa agggattgtg atgggtgcag tcaagggctg agccaagcaa aagggggatt
     4141 tcatcatgag aaggactggc gcgtgtcgag cagatgagct cttgctgtcg atgggcttgc
     4201 gcgagaagat cgggcagatg accatcgtgg gattcccgga gcgcgtcgct ggtgaattgc
     4261 ctgaagggat agagtcgctc ctgaaagacc ccggggtggg cggtgtcggc atctttgccc
     4321 gcaacgcgcg cggacccgct gaaatgctgg agcttacatc agccctccaa gatgccgcga
     4381 tcagggcagg ggcgaaggtt ccgcttttca tcatggcgga tcaagagggc ggcatggtca
     4441 tgcaggtgac agatggaact actgtcttcc cgggcaacat ggctcttgca gcgacgagtg
     4501 atgcagaaaa cgcgcatgag gcttgccggg caatcgccct tgaatctctg gccatgggca
     4561 taaacgttgt gacagctcct gatgtagatg tgaatgctga tcctgataac ccgataatcg
     4621 gggtaagggc ctatggtgat gatcctgcgt tggttgggcg gttcggaagt gccgcggtgc
     4681 gcggatacga ggaaggcgca gtgctgtgca tggccaaaca cttccccggg catggacgga
     4741 ctgctgtaga ttcccatctt gcagtgccgc tcgtagattc gcccatcgaa gaggttgaag
     4801 ccctggatct gccacctttt gaggaagctg ttcgctctgg ggtacgggcg gtgatgacgg
     4861 cgcacattca ggttccggct ctcgatacaa caggcgcgtg cgggacattg tcacgtccga
     4921 tcttgactga acttctccgc gggcgcatgg gatttcgtgg cctgatagtc accgattgct
     4981 tggagatggg ggccatcaag gacacaatag gcacgcccga agccgcggtt cgggcagtag
     5041 aggcaggagc tgacatggtc ctgatatgtc acactcttga gctgcagagg caggcgattg
     5101 acggcatgga gcaggcggtg ctggatgggc ggattcccag ggaggccata gacgcctcgt
     5161 gcaggcggat catcactacc aagctcgagc tcgaagaggc tcgcctgagg atgtggacag
     5221 acggacgtcc tcctctggat gttgtgggat gcagggacca tcttgaggcc gagcagagaa
     5281 tcgctcgtca gtcgatcacg gttttgcgaa acgacgacgg agtgcttccc ttccgcggcg
     5341 atggaccgaa aacgattgct gtcatctacc ccgccaccat gcccaggctt cgggcggagg
     5401 acttggcgga aggtgacaca gtgctcggca aggagtttgc gtcgcggggc gtgacttcgg
     5461 ttgaagtggg cgtaagtctg tgccccacta gggacgagat cgatgcagcc cttgcggcgg
     5521 caggacgcgc agatgcggta gtgatcgcaa catcctgcaa aacccgggaa caagagaagg
     5581 ctcagggtga actcgtgcag atgctgaatg gagcggtaag tgcgcctgtt gtggcgtgcg
     5641 gcattcgaaa cccctacgat atcagggcat atccggaagt cagaacttac ctcgtcacct
     5701 acgggtatcg gccatgttcc atcagagcca tggtggaagt gatgtttggc gagattccgg
     5761 cgaagggaag gcttcctgtg gagataccgg gattctatcc ccgtgggtac gggctcgaga
     5821 tctaggtgga tctcgaatag ttttgcatga tcgtggaatc tatggcatcc aacattcgag
     5881 ctttagctag caaaaacagc tcagcattgt ttgctattca ctcgatttgg cgcatctggc
     5941 gccgatcgag acgaagacaa tttgaagagg gaggctcctg aagctatgtc gacgaatggg
     6001 cgactcgtgt tgtactgcgt aatcggatgt ctcatgctgg cttgctgcgc agctcctgtt
     6061 gcggcaaagg accggaagac tttggcagta tggatgtggg gaagcaccgt gagggcgcag
     6121 ggcgctgaag ctgttgtgaa aagcttggct gcaaacaacg tgaccgacgt gattctgctg
     6181 gtgagaggaa cggctggcaa ggccgaatac gtcagcgcaa tcgcgccccc tgctgaggag
     6241 ggcaaagacg ctctgggcga catcatcgcg gtctgtcgcc cactggggat ccgagtgcat
     6301 gcttggttcg tgttcaacca ggacaagtcc tacaccgatc tgcatcctga agataggatc
     6361 tggcatcatg ggaaggcgaa cactgatttc aagccgtatc cggtggatga cggaaaggtt
     6421 tgccccattt cccagtccca cctggactac acgaatgaca tgatcagaga agtgatcgag
     6481 aactacgacg tcgacggaat tcacctggat tacatacgct acggccacgc ggtctactgt
     6541 ttttgccccg accacgttca aaaggcaact gccctgggaa tcaacgtcga tcgggtccgc
     6601 gaggtcatgt ttgagacttt ctatgcaagc aacgccaatc caaaccgatt cttcgaggca
     6661 tacgagtctg gcgacgcaga tgtcgtcgcc tgggtcaaca tgcgccaggc cgagatacgc
     6721 gatgccgcgg ccagcatgcg cgccgccgcc aagctggcaa agccagatgt gatcttttct
     6781 gcaagcctga tgcctgaagg cgccttcccg gagacaaact cctttgcgat gtgccactat
     6841 ggtcagagct atgaggacgc cggagaactt tacgatttcg tctgccccat ggcctatcat
     6901 cttgattacg gaaaaacccc tgcctgggtt ggggatgtgg cacgtggagc agctaagctt
     6961 gttggcgatg acgtgccagt ctacatagga gttcaggcct atggcgaaaa ggccgatcgt
     7021 gacgcggtgc aaaaggctat cgaggccggc cttggggcag gggccgaggg cgttgctctg
     7081 ttcaggttcg gcactatgaa cgaagaacga tggtcagcgg tgactgacgc tctgaagtag
     7141 tcgcaagcgg agcaaagagg caaggggtgg agctgttgcc ccaccccgtc gctatgggga
     7201 cggtgatggc ggagatgaat gtgaagacgt tctctcacac tgtagtgggg ttggctctga
     7261 ttgtcgtcat gggtctggtt ggggtgaagg ctacagccca aacggcggag ggcgaaggga
     7321 tactcggcct gtttgttcct gtagggacga tatctgacgg ctcttcggca tatgcagagc
     7381 agcttcgcgc ctacaggctt gtgaacatgc ttatgaggtt aggccttccg gcattctgga
     7441 tagctgagcc ctttgagtat gaggacgaca tgcttccgcg cggcagtttc tgggttcccg
     7501 cgcctgacgg caccccttca gttgcagcgc ttgctcaaga acttgcggac aggctaggag
     7561 tcccctatac aatcgtctat gccaggcgct tcgccagcgg cgaaccacgg atttcggctt
     7621 tccgcctcag gcccgtaagc gttgcggtct atcagggcga tcggacatac tcgggcgctg
     7681 ctgacatagc ttcatctctg gagctcgtcg gtgtgtatcc acgttatgtg acagacatcg
     7741 agatcaggcg tggagctctc gatgattatg acatccttgt gatgcctgga ggggctcagg
     7801 atgtgcagtc atacgctctt ggccctgcgg ggaaggcgaa catacgctcg tttgtggaat
     7861 ctggtggtgg ctatctgggc atctgtgcag gggcgtatct ggcgtcagcg ccacaatttg
     7921 agctgaacct tgtagacgct ccatctcaag gacgaggagt tatagggact tggatagctg
     7981 ttcgtcaacg gccgggcagc gatccgctgt tcaggggcta tgatgacatg atatccgatc
     8041 tttattatgg gggtggaccc aatttcgttt ctgatcatcg ggcgatagca gttctcgaag
     8101 gagttcctca gtcttggaaa cccgggctcc agggatcagc tgcggtcatc gaggatacat
     8161 gcgggattgg ttgcgccgct cttgtcgaat cctcctcacg ccactgttgt tcgcgcatgc
     8221 atatccgacc tggagtcatc gctcgatgag atcagctact gcatcggcca gacttgtaac
     8281 atcacggctc gccttgggga taagactgag cgatccaagt tcttctggaa ttactgctac
     8341 ttcggatgga ccatgaagga gccggcaaga cgaatacgcg agcttccagc gaagcttagc
     8401 cggctggcag acgattatga gcaactcgag caggtttccg caacctttga gggtgaagca
     8461 ttcgggtttc caggctacag cgcgggcgaa tgcatgcttc gctcctggaa tgcggtggag
     8521 gctgtctcca gagatgcagc tgtattgcag ccgctgtgga gaagaatctc cgaggacatg
     8581 gcagagatcg aatgcgatgt cgatgatctc cttgcgaagg agaaggcagg ggctacgcct
     8641 accgaactgg actggtggag gctgaacatc agagagtatc tcgtggcaat cgaacttatg
     8701 ggtgggctaa actggaagta ctgggtaagc ggggatagca cggaggagcc cgttcgcgcc
     8761 ggctacgatg acgaatacgg cgatcctacg caaactgtag ccgcctcaac gacgcgcggg
     8821 gcgataggta ccctcacaat gggcgccatg cggctcgacg aggttctaga cgcaaatctc
     8881 gcagctctcg ccatcgcaga gatgatgctt ggcatctgat ccttccgggg gcggccatac
     8941 gcactaagaa gggagcacat caaatggatg gacctcgtcc tcctctcgaa caagagctta
     9001 cagaggtcgt agagcttgta aacacagtct tcagccctga agatcgcacc atggggctcg
     9061 agtttccgca gctgttttct ggaggcaatc tggatcggct cagggttttc gtcgatcaga
     9121 cagcggggat ccctgtggct catggaggat atgttgcaca ggagttggtg gttgaaggtt
     9181 ggcggattcc tgtggcggcg ctcggatcag tctgcactct gccagcctat cggggcaagg
     9241 gccttgcgtc gaggcttgtg gagagcatta tggaccaggc gcggtccgag gggcttgtca
     9301 tgatgctcat atcaggcgac tccaatgtat acaagaggct gggcgctgtt gaaaccggcg
     9361 atttcgtgag ggtagaggca catcaagatc aactttccta tctgtgcgaa attgcgcggg
     9421 cgtggggtga cgacagggag attgtgtgca ggcaggcagg agcggaggac gtgccgtcga
     9481 tggcggccac atacaggcgt gaaccagtgc gatatgcaag atcggtcaag gattttcatg
     9541 aattggcctg gtggaggtct gacgtcaagc gtctttacgc ctatgagagc attcaagtgg
     9601 catgtaccgg gcgggaaacg ctggcttaca ttgttacacg cgtgcgcacc ctggacggcg
     9661 gaggagtaag gctgcatgtg cccgagttcg cggggcctag atgggctgtg gcttgtctgg
     9721 tggggcatgc ctgcacagcg ttttcgccca acgtgcttac gatgacggtt tgctcatcag
     9781 atcgcgacat ggttgaaacg ttgcggcggg ctgcattgcc cttgcgtaga acaacgttgc
     9841 ctggctacac agtgatggcg ttggattctt ctgcacttct gagtagcgct ggatccatca
     9901 tttccgaaag gggcggcgct gcgcgggatg attcgctgtc gcgactcttg gcgatctcag
     9961 gtgccgatct tgtgaagacg ttattcgggg gcacggatgc gatcccgttg cctgttccgg
    10021 gtttgaattt catatagccg gagggttttg aatggacgtt gtaataggcg ctgatggcgg
    10081 gggaaccaag accgcctgcg ccatagcaca aatggacgga aacatcattg gactgggtta
    10141 cgctggacca tccaatcatc tgcgcctgcc cggaagccat gcacaagcgg cagagaccgt
    10201 gctcgaaggc gtacaggcag cattttccga tgcggggata gcagttatcc gggctcgcca
    10261 ggcctacctt gcgcttggag gcgttgatgt tgatcgccca aatgagaaga tggcagaggc
    10321 ggcccgccag gtcttggacg cagaagagat agcaactgac aatgatgcgg cggcaggcct
    10381 tgcggctgcc acgggtgagt tgtacggtgt ggtcatcatt gccggaacag gaagtattgc
    10441 catggctgtt gacggggaag gcaggcggcg taggtctggc ggatgggggt atcgcatcgg
    10501 agatgaaggt agcggccagt ggataggacg cctgggactt gaagctgcgg ctaaggctca
    10561 tgacggccgt gggaagccaa catccttagc agacagggtg ttggatcatt tcgggataga
    10621 aaccgttgat ggactccgat ctgtcctcta taagcagccg gtaaacaaca gggacatcgc
    10681 cgatttctgc atgcaggtaa tggcttcggc ccgggagggc gatgaggttg ccctcgacat
    10741 cgtgaaccgg gcagggcgcg aattggccct tgccgtgtgg tctgctgcag atggacttgg
    10801 catggcttcg gccgagtttc cagtagttcg ccagggaagc gtgttcaagg cgggcgatgt
    10861 cattgcgcgt cctttcgagg aagagcttgg ccggctcgcg cccgcagcca cagtggtcag
    10921 cgcggcattt ccgccagtgg ttggggcggt tatgctggca ttgatgcggg caggcgttga
    10981 gattgacggt gctgtgcggg aaaacctcgc aaggtcatgg gaggaggttg aggcgcgcca
    11041 tgagcgtcta tcttgaggca gctgttgaca tgaaggacga aattgaagag atttggactg
    11101 cactccatgg gatgccggag cctccatttc aggagacgaa aactgcgggt ttcatagcat
    11161 acaggctttc ggaagccggg tttgatgtga agaagggcct tgcggatacg gggatagtgg
    11221 ctgacttagg agatcctgct gcgggtgatc tgacgggacc ggtgattgcc cttcgggccg
    11281 atatggacgc aatgaggcat gacatagatg gacgcgaggt ttgggttcat tcctgcggtc
    11341 acgacgctca ttcgacaata gtcataacgg caggactcat gctggcccgg cggtgtgccg
    11401 gggccagagg caggctaaga ttgatctttc agcccgggga agagacaggg ctgggcgctg
    11461 gcaggatgat agaagcgggc gcagtccggg gagtagatgc cttgctcgga atgcacctgc
    11521 ggcctgtgtc agagtgccgt gtggggcagg caacaccttg tctttggcat tcggcaggga
    11581 gaacccttag ttactcagtg gttgggaaac cgtctcacac ggggcggcct caccttggga
    11641 taaacgtggc ggacgctgtg gctatggcaa ccttggctgt aaataatgtc aaacccgatc
    11701 cgcttaggtg tgcgactgtc aacgtagttg cgttgagtgc gggagatggg ccaggcggcg
    11761 tcataccgga gaggggaagc ctgacggtga acatcagaag cgagtctgat cagatcgcag
    11821 atgagcttag ttccaaggtg gatggggcgg tcagggctgt cgtggctgct aatggcgccg
    11881 aggcagggct gaaaagagaa aagcagatgc cggccccacg ctatgatgag ggcatgatcg
    11941 ctctggcgcg ccgggctata gtctcggtgc tgggagaggg aggcgttgta gagaagatca
    12001 cgactcctgg gtctgaggat tttcactggt atgcaagg
//
LOCUS       DAOSAI010000024        11729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3680_length_11729_cov_3.856176, whole genome shotgun sequence.
ACCESSION   DAOSAI010000024 DAOSAI010000000
VERSION     DAOSAI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3680_length_11729_cov_3.856176"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2874)
                     /gene="rpoB"
                     /locus_tag="PLM83_01815"
     CDS             complement(<1..2874)
                     /gene="rpoB"
                     /locus_tag="PLM83_01815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOK70178.1"
                     /translation="MDIAERPMRRERISFSRIADVLEVPNLIEIQQKSYQWFLEEGLL
                     ETFRDISPVQDFTGNLVLEFTGYALGDPKYTEEECKQRDVTYSAPLKVKVRLINKETG
                     EVKEQEVFMGDFPLMTETGTFIINGAERVIVSQLVRSPGAYFDVDKDSSGRTIYKASI
                     IPNRGAWLEFETDASDIVWVHVDRTRKLPVTVLLKAIGYGSAQEILELFGENELIRTT
                     LERDNTNNEEEALIEIYKRLRPGEPPAIESARSLFESLFFDPKRYDLAGVGRYKLNKK
                     LELADRLIGHVLARPLYGDIVENTNTVRTVIDPETGEISWLDMSDGGIDSGRKPALEA
                     GEKITARRAELVRELEENVHIREASLVLPDGDPQDFIKVIGNGYPPADLKVITPADIV
                     ATVNYLVNLPYGIGTIDDIDHLGNRRLKTVGELLQNQFRIGLSRMERVIRERMTIQDV
                     EAVTPQALINIRPVVAAVKEFFGSSQLSQFMDQTNPLAELTNKRRLSALGPGGLSRER
                     AGFDVRDVHHSHYGRMCPIETPEGPNIGLIGSLCTYARINEYGFIETPYRKVEDGRAT
                     AQIRYLTADVEDRYIIAQANEPLNEDGSFVSPRVACRLKDEIVIVAADQVDYMDVSPK
                     QLVSIATALIPFLENDDAGRALMGSNMQRQAVPLIKTEAPFVGTGMEYKSAVDSGVVL
                     VAKRGGYVRHVSADHIVIERDDGVEDRYNVIKFARSNQGTCMNQKPIVKIGQRVEAGD
                     VIADGPSTDMGEMALGRNVLIAFMPWEGYNYEDAILISEKLVSDDVFTSIHIEEYECD
                     ARDTKLGPEEITRDIPNISEDALKDLDEEGIVRVGAEVRPGDILVGKVTPKGETELTP
                     EERLLRAIFGEKAREVRDTSLRVPHGEAGKVVEVHVFSRENGDELAPGVSKLVRVYVA
                     QKRKVSEGDKMAGRHGNKGVIARILPIEDMPFLPDGRPVEIVLN"
     gene            complement(3300..6731)
                     /gene="rpoB"
                     /locus_tag="PLM83_01820"
     CDS             complement(3300..6731)
                     /gene="rpoB"
                     /locus_tag="PLM83_01820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOK70179.1"
                     /translation="MKRERILFSKVPSVLELPNLIEVQHKSYQWFLGEGLREVFRDIS
                     PVQDFTGNLVLEFTGYALGDPKYTVEECKRRDVTYSAPLNAKVRLISKATGEVKEQEV
                     FMGDFPLMTEAGTFIINGAERVVVSQLVRSPGAYFDIDRDSSGREIYTASIRPNRGAW
                     LEFETDASNMVWVRVDRTRKLPATVMLRAMGLSSDEEIIEVFGDNEYIRNTLERDNTS
                     NEEEALIEIYKRLRPGEPPSIDSARSLFEFLFFDPRRYDLAKVGRYKLNKKLELADRL
                     IGQRLAEPIHYPVTNGTDPARAVVDSETGEITWLDDAGEEVDLGTKLALEAGEKVTAR
                     RAELVRELEQQGHIGEAILVAPDDAPQDSIKVIGNRYPPANLVVITPADVVATVNYLV
                     NLPYGIGTIDDIDHLGNRRLKTVGELLQNQFRIGLSRMERVIRERMTIQDVEAVTPKG
                     LMNVRPVVAAVREFFGSSQLSQFMDQTNPLAELTHKRRLSALGPGGLSWRRAGFEVRD
                     IHHSHYGRICPIETPEGPNIGLIGSLCTYARVNEYGFIETPYRRVEDGKVTEEIRYLT
                     ADMEDRYVIAQANEPMNEDGSFASQRVACRLKDEIVIVPVDQVDYMDVSPKQLVSIGT
                     ALIPFLGNDDAGRALMGSNMQRQAVPLLSPEAPLVGTGMEYKSVLDSGAVIVAKHGGF
                     VRSACADRIVVERDDGRLDTYNVTKFARSNQGTCINQKPLVRVGHRVEPGDVIADGPS
                     TDMGEMALGRNILVAFMPWEGYNYEDAILISEQLVIDDVFTSIHIEEYECDARDTKLG
                     PEEITRDIPNIGEDALKDLDEDGIIRIGAEVQPGDILVGRVTPKGETELTPEERLLRA
                     IFGEKAREVRDTSLRVPHGESGKVVEVHAFSRENGDELAPGVGKLVRVYVAQKRKISE
                     GDKMAGRHGNKGVIAKILPVEDMPFLPDGRPVEIVLNPLGVPSRMNIGQVLETHLGWA
                     AAALGVHVATPVFDGADEDDVFGYLEKAGLPANGKTILYDGRTGEPFDREVTVGYAYL
                     MKLLHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQEM
                     LTVKSDDVLGRVKTYEAIVKGDMIPEPGVPESFKVMIREMQGLCLDIQVLPADEE"
     gene            complement(7430..7810)
                     /gene="rplL"
                     /locus_tag="PLM83_01825"
     CDS             complement(7430..7810)
                     /gene="rplL"
                     /locus_tag="PLM83_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOK70180.1"
                     /translation="MTKEEILSAIKNMTVLELSELVKDLEEEFGVSAAMPVAAMASAG
                     AAGAAAAPEEEEKTDFDVILVSPGEQKIPVLKVVREITGLGLKEAKELVDNAPKPIKE
                     GVKKEEAEEIKAKIADVGGQVEIK"
     gene            complement(7829..8380)
                     /gene="rplJ"
                     /locus_tag="PLM83_01830"
     CDS             complement(7829..8380)
                     /gene="rplJ"
                     /locus_tag="PLM83_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOK70181.1"
                     /translation="MPTPKKIQTVEEIQSKLNEASCVVLTDFRGLNVAQATALRRRLT
                     EAGVEYKVYKNTLIKIAADNVGIEGLDSYLQGPTALAFSTEDPIAPAKILADFARETK
                     ILKVKAGVLDGQIIDKDRVAELAALPPKEVILGKVVGAMQGPIFGLVNVLAGPTRKLV
                     WALDAIRQQKEEAGGDPQTVQAQ"
     gene            complement(8627..9337)
                     /gene="rplA"
                     /locus_tag="PLM83_01835"
     CDS             complement(8627..9337)
                     /gene="rplA"
                     /locus_tag="PLM83_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOK70182.1"
                     /translation="MPKRGKKYQDSVKVIDRMTLYGPAEGVKLVREAAKANFDETIEA
                     HIRLGVDPRRSDQQVRGAVVLPYGTGKTKTVLVFAMGEKAREAEEAGADYVGGEDLAQ
                     KIQGGWLDFDAAVATPDMMSVVGRLGKILGPRGLMPNPKTGTVTFEVAKAINEIKAGK
                     VEYRVDKSGIIHVVIGKASFEEGMLIANLRVLSDAIVKARPAAAKGTYIKSVALAPTM
                     GPSVRLNPQLFAQMVAEK"
     gene            complement(9434..9862)
                     /gene="rplK"
                     /locus_tag="PLM83_01840"
     CDS             complement(9434..9862)
                     /gene="rplK"
                     /locus_tag="PLM83_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOK70183.1"
                     /translation="MAKKVTAVVKLQLNAGKASPAPPVGPVLAPTGINIVEFCKNFNA
                     KTADQVGTIIPVEVTVYDDRSYTYILKTPPVSFLIKKALGIEKGSGEPDTRKVGKLTL
                     DQVRQIAEVKMPDLNANDIEAAMELVKGSARSMGVEVAEA"
     gene            complement(9890..10444)
                     /gene="nusG"
                     /locus_tag="PLM83_01845"
     CDS             complement(9890..10444)
                     /gene="nusG"
                     /locus_tag="PLM83_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOK70184.1"
                     /translation="MSKMGTDKIENGRGWYVVHTYSGYENKVKTNLERRVQTMEKEDK
                     IFRVLVPTEEVTEFKDGRKKVVQRKRFPGYVLVEMVMSDDSWYVVRNTPGVTGFVGSG
                     NKPIPLTEEEMHAILRQMGVEEPKVTVTFEIGETVRVISGPFENLDGIVQEVSPERGR
                     LRVQVSMFEREIPVELDFTDVEKY"
     gene            complement(10441..10680)
                     /gene="secE"
                     /locus_tag="PLM83_01850"
     CDS             complement(10441..10680)
                     /gene="secE"
                     /locus_tag="PLM83_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOK70185.1"
                     /translation="MAQEAKPTLSTRMKGFFGKIGKFFREVRAEFKRVQWPSRKETWS
                     LTLAVIVYVLIVTVFLGLVDLGLSFLVEQIVKAAA"
     gene            complement(10722..10871)
                     /gene="rpmG"
                     /locus_tag="PLM83_01855"
     CDS             complement(10722..10871)
                     /gene="rpmG"
                     /locus_tag="PLM83_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOK70186.1"
                     /translation="MREGITLQCSECKNRNYQTAKNKRNDPDRIELKKYCKFCRAHTL
                     HKETR"
BASE COUNT         2545 a         3590 c         2672 g         2922 t
ORIGIN      
        1 attgaggact atctcgacgg gacgcccgtc cggtaagaac ggcatgtcct ctatgggcag
       61 gatgcgggcg atgacgccct tgttcccgtg gcggcccgcc atcttgtcgc cttccgagac
      121 ttttcgcttc tgagccacat agactctgac aagcttgctc acgcccggcg caagttcatc
      181 tccgttttca cgggaaaaaa cgtggacctc cacgactttc cctgcttcgc catgaggcac
      241 cctgagggaa gtatcgcgca cttcgcgcgc cttctcgccg aatattgccc ggagcaagcg
      301 ctcttcaggc gtcagttccg tctcgccttt cggcgtaacc tttcccacaa gtatgtcgcc
      361 gggcctcacc tctgcgccga ccctaactat gccctcttcg tccaggtcct tcagggcatc
      421 ctcacttatg ttgggaatgt cccgggttat ctcttccgga ccaagtttcg tatcacgggc
      481 gtcgcattcg tattcttcta tatggatcga tgtgaacaca tcatcgctaa caagtttctc
      541 tgatatgagg atagcgtctt cgtagttata cccttcccac ggcatgaacg cgatgagaac
      601 attgcgcccc agcgccatct ctcccatgtc agtggatggt ccatcggcaa tcacatcgcc
      661 cgcctcaacc cgttgaccga tcttgactat gggtttttgg ttcatgcacg tgccttggtt
      721 ggaacgggca aacttgatca cgttgtatct gtcctccacc ccgtcatctc tttcaatgac
      781 gatgtggtcc gcagacacat gacgcacgta tccgccgcgc ttggccacta gcaccacccc
      841 ggaatcgaca gcagacttgt attccatccc tgttcccaca aaaggagctt cagtctttat
      901 gagcggaaca gcctgtcgct gcatgttcga acccataagg gcacgacctg catcgtcgtt
      961 ctccaggaat gggatcaaag ccgtcgcaat agagaccagc tgtttcgggg agacatccat
     1021 gtagtctacc tgatccgctg cgactatcac gatttcatcc ttcaagcggc acgccactct
     1081 cggactgacg aaagacccat cctcgttcag gggctcattc gcttgagcta tgatgtatct
     1141 gtcttccaca tcagcggtga gatacctgat ctgcgcagtc gcccttccgt cctctacctt
     1201 tctataaggg gtctctataa agccgtactc gttgatgcga gcatacgtgc aaagcgaccc
     1261 tatcagcccg atattcggcc cttcaggcgt ctcgatcggg cacatcctgc cgtagtgcga
     1321 atgatggacg tcacgaacat cgaacccagc cctttccctg gaaagccccc cagggccaag
     1381 tgccgatagc cgacgtttat tcgtcaattc cgctagcggg ttggtctggt ccatgaactg
     1441 cgacagctgg cttgatccga agaactcctt caccgccgct accactggtc ttatgttaat
     1501 gagcgcttga ggcgtcacgg cctccacgtc ttggatcgtc atgcgctcgc gtatcactcg
     1561 ctccatgcgt gacagaccga tcctgaactg gttctgcaaa agctcgccaa cggtcttcag
     1621 ccttctattg ccaagatggt caatgtcatc aatcgtgcca atgccatacg gcagattgac
     1681 aaggtagttg actgtggcca ctatgtccgc aggcgtaata acctttaggt cggccggggg
     1741 atagccattt ccaatcacct taatgaagtc ttgtggatct ccgtccggca aaacgaggct
     1801 agcttcccgt atgtgtacat tctcctcaag ctctcgaacg agctcagccc ttcgggccgt
     1861 tatcttctcc cccgcctcca aagccggctt gcgccctgaa tctatcccgc cgtcactcat
     1921 atcaagccag gagatttctc ccgtctcagg atcaataact gttctgactg tgtttgtgtt
     1981 ctccacaata tcgccataca aaggtcgggc aagcacatgc ccgatcagtc tgtcggcgag
     2041 ttcaagtttc ttgttgagct tgtagcggcc caccccagcc aagtcatacc tcttggggtc
     2101 gaaaaagagc gattcaaaaa gagatcttgc actttcgatg gccggaggct cgcctggccg
     2161 aagtcgctta tagatttcga tgagcgcctc ttcctcgttg ttcgtgttgt cgcgctccag
     2221 agtagtgcgg ataagttcgt tctccccgaa aagctctaga atctcctgag cagaaccata
     2281 tcctatcgcc ttcaacagga ctgtaacagg gagttttctt gtgcggtcta catgcaccca
     2341 aacaatgtca gaggcatcag tttcaaactc gagccatgcc cctctattcg gtattatgga
     2401 cgccttatag attgttcttc cggatgagtc cttatccacg tcaaagtaag cacccgggga
     2461 cctcaccaac tggctgacga ttacgcgctc agccccatta atgatgaacg ttcccgtctc
     2521 ggtcatcaat gggaaatccc ccatgaagac ttcctgctct ttgacttctc cagtttcttt
     2581 gttgatgagc ctgaccttta cctttagagg agcggaatag gttacatccc gctgtttgca
     2641 ttcctcttcg gtgtatttcg gatcgcctag agcataccct gtgaattcga gcacaaggtt
     2701 tccagtaaaa tcctgcaccg gcgagatatc tcggaacgtt tcgagaaggc cctcttcaag
     2761 aaaccattgg taggacttct gctggatctc gatgaggttt ggaacttcga gaacgtccgc
     2821 aatcctggaa aacgaaatcc tttcacgcct catcggtctc tcggcaatgt ccatacaacc
     2881 caccctactt ccttgtagat tgtagatttc cgggcataac gaatagcgcg gacattaatg
     2941 acctttggcg catatcctcc agtcaaagcc gcgctgagac taatagatca tacctgcaaa
     3001 tagcatgtgt gttcatttgt ccccttatac ctcagtcctc cgtcactcag ggcgtgcccg
     3061 cgccgctcca tctcatgaaa gcttatgccc cagcagatca cggcgcatca actctgctca
     3121 tgcccttgtg acattttatt atgctaccac aagagtgacg ccccgtcaag accaatttat
     3181 ctgtttttta agcgctgatc aggtcttgtt gataacaccg cctctgctgt aaaacgccta
     3241 cctccaaggc ggacccatga atgtgaagat tccaaaaccc gccacatgca cgtaattgct
     3301 cattcctcat ccgcaggcaa gacctgtata tcaaggcata ggccctgcat ctctctgatc
     3361 atcactttga aggactcagg aacgccaggt tcaggaatca tgtccccttt tacaatcgcc
     3421 tcatatgttt tgactctacc caaaacatcg tctgacttta cggtgagcat ctcttgaagc
     3481 gtgtgggcgg ccccataggc ctccaatgcc caaacctcca tctcaccgaa tctctgccct
     3541 ccgaactgag ctttgccgcc cagtggctgc tgggtcacta gggagtatgg tcctgtggaa
     3601 cgcgcatgga tcttgtcgtc gacaaggtgt agtagtttca tcaagtaggc gtagcccaca
     3661 gtcacctctc ggtcgaatgg ttcaccggtt ctcccgtcat acagaatagt cttgccattt
     3721 gcgggcagac ctgctttctc cagataccca aaaacgtcgt cctcatcagc accgtcgaac
     3781 acaggcgttg ccacgtgaac gccgagagcg gctgcggccc agccaaggtg agtctccagc
     3841 acctgaccga tattcatccg cgaaggcact cccaacggat tgaggactat ctccaccgga
     3901 cgcccgtccg gcaaaaacgg catgtcttcg accggcaata tcttggcaat cacgcccttg
     3961 tttccgtggc ggcccgccat tttatcgccc tcggagatct tccgcttctg cgccacataa
     4021 actctgacaa gcttacctac acccggagca agctcgtctc cgttctcacg ggaaaacgca
     4081 tggacctcta ctaccttccc tgactcgccg tggggcaccc tgagcgaggt atcgcgcact
     4141 tcacgcgcct tctcgccgaa tattgcccgg agcaagcgct cctcaggcgt gagctcagtc
     4201 tcgcccttcg gcgtaaccct tcctacaagg atgtcgccgg gctgcacctc tgcgccgatc
     4261 ctgattatac catcctcatc caagtccttc agggcgtcct caccgatatt cgggatatcc
     4321 ctcgttatct cctcaggtcc aagcttagta tctcgagcat cacattcgta ctcctcgatg
     4381 tgtatggatg taaagacatc gtcgatcacg agttgctcgg atatgaggat ggcgtcctca
     4441 taattgtagc cctcccacgg catgaacgcg actaaaatgt ttcgtcccag cgccatctcg
     4501 cccatatcag tggatggtcc atcggcaatc acgtcgcctg gctcgactct atgaccaact
     4561 ctgaccaaag gtttctggtt aatgcatgta ccctggtttg atctggcgaa tttggtcaca
     4621 ttgtatgtat ccaatctgcc atcatcccgc tcaaccacga ttcgatcagc acacgcactt
     4681 ctaacgaatc cgccatgctt ggccacgatc accgccccag aatcgagaac cgacttgtat
     4741 tccatgcctg ttcccaccag cggggcttcc ggactcaaaa gcggaaccgc ctgtctctgc
     4801 atgttcgagc ccatcagagc ccgaccagca tcatcattcc ccaagaacgg gataagggca
     4861 gtaccaattg aaaccaactg ttttggcgag acatccatgt aatctacctg atcgaccggg
     4921 actattacga tctcatcctt caagcgacat gctactcgct gactagcaaa agaaccatcc
     4981 tcattcatgg gctcattcgc ctgggcgatg acgtatctgt cttccatatc ggcagtgaga
     5041 tatctgatct cctccgtcac ctttccgtcc tcaacccgcc tataaggcgt ctcaatgaaa
     5101 ccgtattcat tgacacgggc atacgtacac aaagacccta tcaacccgat gttaggccct
     5161 tcaggcgtct cgatcggaca tatcctgcca taatgagaat gatggatgtc gcgcacttcg
     5221 aacccggctc tcctccacga cagccccccg gggccaagcg ctgacaatcg gcgcttatga
     5281 gtcagttcgg ccagcggatt tgtctggtcc atgaactgcg acagctggct cgatccaaag
     5341 aactccctta ccgcggccac cactggcctg acattcatca agcccttagg cgtcacagct
     5401 tcaacatcct gaatagtcat gcgctcgcgt atcacccgtt ccatgcggga taatccaatc
     5461 ctgaactgat tctgcagcag ttcgccaacg gtcttgagcc ttcgattacc aaggtggtcg
     5521 atatcatcaa tcgtgccaat gccatatgga agattgacga gataattcac agtagccaca
     5581 acgtccgcgg gcgtaattac aacaaggttg gcagggggat atcgattgcc gataaccttt
     5641 atggaatctt gcggcgcgtc atccggagca acaagaatcg cttctcctat atgtccttgc
     5701 tgttccagct cccgaacgag ttcggctcgt ctggcagtaa ccttctctcc tgcttccaac
     5761 gccagcttgg tccctagatc tacctcctcg cccgcgtcat caagccaggt gatctctccc
     5821 gtctcggaat ccactacggc ccttgccgga tctgtgccat ttgtaaccgg ataatgtatg
     5881 ggctcggcca gcctttgacc gatcagcctg tcggcaagtt ccagcttctt gttgagtttg
     5941 tagcggccca ccttcgccaa gtcatatctc ctgggatcga agaaaagaaa ctcaaacaaa
     6001 gacctcgcac tatcaataga aggcggctcg cctggcctaa ggcgtttgta gatctcgatc
     6061 agggcctctt cctcgttact cgtgttgtcc cgttccaacg tattgcggat gtactcgttg
     6121 tctccgaaaa cctcgatgat ctcctcatct gagctaagcc ccatcgccct caacatgacc
     6181 gtggcaggaa gctttctcgt ccggtcaacg cgcacccaga ccatattcga ggcatccgtc
     6241 tcaaactcaa gccaagcgcc tcggttaggc cttatggatg ccgtgtagat ctcccttccg
     6301 gatgaatccc tgtcaatgtc aaaataggcg cctggggatc tcaccagctg gctgacgact
     6361 acgcgttccg ccccgttgat aataaatgtg ccagcctcgg tcatgagcgg aaagtctccc
     6421 atgaaaacct cctgttcttt gacctctcca gttgccttac tgatcaacct gacctttgca
     6481 ttgagtggag cagaataagt aacgtcccgt cttttgcatt cctccaccgt atatttcgga
     6541 tcgcctaagg cgtatccggt aaactcgagg acaaggttgc cggtaaaatc ctggactggc
     6601 gagatatctc ggaacacttc gcgcaggccc tctccgagaa accattgata tgatttgtgc
     6661 tggacctcga tgaggttggg gagttcgagg acactgggga ccttggaaaa caggattctc
     6721 tcgcgtttca tcggcttagt cgcaatctca atagacccca ccctactttc taacggcttt
     6781 tcagatacaa gaaacagtgc ggaccatcat gacatggacc aaacgcccat aagccagagc
     6841 cgcactgaga ctggcagatc tcacgtgcaa aacatcagcg ttttgccgcc ctctcataac
     6901 ctattcactg gacacttgcg cgaagccaaa gctcggctcc gcaagggctg ggcgcccgca
     6961 aatcacagcg cactcgatct gcccgtcctt cggcatcatc ctttatgtta acatatagcc
     7021 cgaatcgcgt caaatcatgc cagctagagc ccttacagcc attcttggat cacaaaaccc
     7081 acatttggaa tacaacgcac tccctttctt cccgcctgta gcttttggca taacgtcatt
     7141 gctccagaga gcataatatg tttactgtga agttgagttt ctcttacaac tctccgagga
     7201 tacccgatgc gaacctgaga atggaattag tgattattgc ggggaaaaga gacacgcaag
     7261 gctgcgctag gaccggttgg gaggaagctt tggcgaaaca cgagcctgct caatatccac
     7321 agcctgaaac tggcacagat agaatcgccg ggagtcggcg tgtgcgatgt ccgcattgcc
     7381 ttcccccggc atagattcga ctttttcagt gacttcaagg ctcagcagac tacttgatct
     7441 cgacctgacc tccgacatcg gcaatcttgg ctttgatctc ttccgcttct tccttcttta
     7501 cgccttcttt gatgggctta ggcgcattgt caacgagctc cttggcctct ttaaggccga
     7561 gcccagtgat ctccctgact accttgagca caggaatctt ctgctctccc ggagaaacca
     7621 gaattacatc gaagtctgtc ttctcttcct cttccggagc agcagcggcg cctgccgctc
     7681 ctgccgacgc catagcggcc accggcattg ctgcgcttac accaaactct tcctcgaggt
     7741 ccttgaccaa ttcggacagc tcgagaactg tcatgttttt aatagctgaa aggatttctt
     7801 ccttagtcat agcacatttt cctccctctt attgagcctg aacggtctga ggatctccgc
     7861 cggcttcttc cttttgttgc cttatggcat ccaacgccca tactagtttg cgcgtgggac
     7921 cagcaagcac attgaccagg ccaaaaatcg gtccctgcat tgccccgacc accttgccaa
     7981 ggatgacttc cttgggaggc aaggctgcaa gctccgcgac ccgatccttg tcaatgatct
     8041 gtccgtccaa aacacccgcc ttgaccttga ggatcttcgt ctcacgggca aaatcggcaa
     8101 ggatcttggc cggagcgatc ggatcctcag tggaaaaggc aagcgcagtc gggccctgaa
     8161 gatatgaatc tagcccttcg atgccaacgt tatccgctgc aatcttgatg agcgtattct
     8221 tgtagacctt gtactccacg cctgcctccg taagccttcg tctgagcgca gtcgcctgcg
     8281 ccacattgag accacggaag tcagtcagca caacgcagct cgcttcgttc agttttgatt
     8341 ggatttcttc aacagtctgg atctttttcg gtgtaggcac tttcacacct ccgttcgcac
     8401 gcgtacgatt cgcttagtac tggcaataga aagggacctc ggctcaggta tggccggagg
     8461 tcccgcaaat cacagacata tatttgcgtt cggaagccaa gcttccctcc agcctcggcg
     8521 ggctgacatt tcgtcactta ggccgcgttt cgtgcagcgt tgccacacta cgcgacgcct
     8581 gctgtcttgg gccaggcaca tctctctatt gtcaaagcag acaactctac ttttcggcca
     8641 ccatctgcgc aaacagctgg ggattgagac gaaccgacgg acccatcgtc ggcgccagcg
     8701 cgacgctttt gatgtaggtc cccttggcgg ctgccggacg agctttcact atggcgtccg
     8761 acaacaccct gaggttcgcg atcagcatcc cctcttcaaa tgaggccttg ccgatgacaa
     8821 cgtggataat gccactcttg tcgaccctgt attcaacctt acccgctttg atctcgttga
     8881 tcgctttggc cacttcgaac gtgaccgttc ctgttttcgg gttcggcatc aagccccttg
     8941 ggccaaggat cttacctagc cttcccacga cactcatcat gtctggagta gctacggcag
     9001 catcgaaatc gagccatcca ccctgtattt tctgagccag atcttccccg ccaacataat
     9061 cggcgccagc ctcttctgcc tcgcgcgcct tttcgcccat tgcgaaaacc agcacagtct
     9121 tggtcttgcc agttccgtac ggaagcacga ccgccccacg gacttgctga tccgaacgac
     9181 ggggatccac acccaatctg atgtgggctt cgatagtctc atcgaaattg gccttggccg
     9241 cctcgcgaac gagtttcacg ccttcggccg gtccatacag agtcatccta tcgatgactt
     9301 taacggaatc ctgatacttc ttgcctctct ttggcatagg ttttacctcc cacgtggtgc
     9361 cagcggaaca tgtcctccca cgattgtagc ccgcaacatt caagcgcagg ctgttgcgga
     9421 taggttgtaa ggcctaggcc tctgcaacct ccacgcccat acttctggcg ctgcccttca
     9481 ctagttccat ggcagcttcg atatcatttg cattgaggtc aggcatcttt acttctgcaa
     9541 tctgcctaac ctgatccagg gtcagcttgc cgacctttcg agtgtcgggt tcgcccgaac
     9601 ctttctcaat cccaagcgcc ttcttgatca agaaagacac cggaggcgtc ttgaggatgt
     9661 aggtgtatga cctgtcgtcg tacactgtga cctctactgg tattatcgta cctacctgat
     9721 ctgcagtctt ggcgttgaag ttcttgcaaa actcaacaat gttaattccg gtcggcgcca
     9781 gcacaggacc gacaggcggt gcggggctag cttttcccgc attcaactgc agcttgacta
     9841 ctgccgtgac ttttttcgcc attggctgac acctcctccc cggcgagtgc tagtatttct
     9901 cgacatcagt aaaatctagt tccaccggga tttcgcgttc gaacatagat acctgtacgc
     9961 gcaatctgcc cctttcgggg ctgacctcct ggacaattcc gtcgaggttc tcaaaaggac
    10021 cagagatcac cctgacggtc tcgccaatct cgaaggtcac cgtgaccttg ggctcctcca
    10081 cccccatctg acggaggatc gcatgcattt cctcttcggt aagtggaatc ggcttgttgc
    10141 cggagcccac aaaacccgtg actccgggcg tgtttctgac gacataccaa gaatcgtcac
    10201 tcatcaccat ctcgaccagg acataaccgg gaaatctctt tcgctggaca acctttttac
    10261 gcccatcctt gaactcagtt acttcttcgg tggggacgag cacccggaag atcttgtcct
    10321 ctttctccat ggtctgcacg cgccgttcca gattggtctt cactttgttt tcatagcctg
    10381 agtacgtatg caccacgtac cagcctctgc cgttctcgat cttgtcagta cccattttgc
    10441 tcatgcagct gccttgacaa tctgttcaac caggaaggat agtcccaggt cgactaggcc
    10501 cagaaacaca gttacaatga gcacatacac gatcacggcc aaagtcaaag accacgtttc
    10561 ctttcggcta ggccactgaa ctcgcttgaa ttcagcacga acctcccgaa agaacttccc
    10621 aatcttgccg aagaatcctt tcatgcgcgt ggagagagtc ggcttggcct cttgagccat
    10681 gaaatagatc cctcccctgt gaaacacggc tagattatac cctagcgtgt ttccttgtgt
    10741 aaagtgtgcg ccctacagaa cttgcagtac ttcttcagct cgatcctatc agggtcattc
    10801 cgcttgttct tggcggtctg gtagttgcgg ttcttgcatt cactacactg aagggtgatt
    10861 ccctcccgca tctcgttaga cctccccatt actcagttgc atcagctgtc gcccgaaggc
    10921 tcgagcagac acctatgacg ccccccgtca ggagcgccca taatcacccc taaaagttat
    10981 cataccccac cagcactgtc aatccacccg aaacaccgcc tagcgtcgat agtctgccgt
    11041 gcgcaagatg aatgcggaca ttgcagccca cactgagagt aaactgcaag cgccaactac
    11101 ccggtcatgc tgacagcagg caacgcctat gcatttcatc gcaggctagg gtgactaatg
    11161 tgcagccagt gcacctgcat cgacatgaac ggggcagatc attcacgctg aagtatcatg
    11221 gtgccaggca atccgcctag cgtcatccct ctcaaagaac cacgcctgca ggtctcatac
    11281 acggtttcta acaagcctta gtcacttcgt tccattgaaa agtgaaaaga tgcaatgcac
    11341 cgaccacgta gttggtgagc tccttcatcc ccacgattcc tttgttcctt tcttgcatcg
    11401 ctggttatat ccacagtccc gcaaggcgtt tttagcttga aggcaatcta ccccgcccgg
    11461 ctttgagcgg gcacaggacc cagcagctcc ggatctctgt cccctcagcc ttatcctcca
    11521 gccttttgcc agcttgtcgc aggctgaatc ctacagactc atctaccgcc ctacttccat
    11581 ccttgcgtct ccgcttcttt aggcctgcca gctctctgga tgatgcagag cttccccggt
    11641 tgagtttgtc tgcctcaacc gtccgttcaa ggctcccagg ttatccagct ttcgggcttt
    11701 cccatacttc gcaaggttac ccacctgac
//
LOCUS       DAOSAI010000025        11696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3687_length_11696_cov_4.234688, whole genome shotgun sequence.
ACCESSION   DAOSAI010000025 DAOSAI010000000
VERSION     DAOSAI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3687_length_11696_cov_4.234688"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLM83_01860"
     CDS             complement(<1..480)
                     /locus_tag="PLM83_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOK70187.1"
                     /translation="MKILIASIDEKDLIGAKDYGVHGIITNPTIVAEVRKPWKEAVSD
                     AAKIIEGPFHLQITEDKRDGIIAQAEEFASVLGDRLIVKMCISQESLAAMQVLQDRGL
                     KVNLTGIVSIPQSFFAIQAGADFISIYMGRADDVGGDGVGVVRSAANMAKNGGYKTEI
                     "
     gene            complement(585..1565)
                     /locus_tag="PLM83_01865"
     CDS             complement(585..1565)
                     /locus_tag="PLM83_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOK70188.1"
                     /translation="MEIEQNPSSEHELLVRVARMYYEHDLNQQEIAERVGLSRSRISR
                     LLTRAREVGIVQVTIVDEFQEEHGLSRELESMYGLKRVVVCPISESSPKPLRWHLGQA
                     AARLLKEIIRDGDVIGVTGGTTMLEMARALKPLPIKRENVKIVQLEGALSGKGEALIH
                     GNEIVAKVAAAFDAPPYFLSAPTIVESRELRDALARDPNLRRILEKGRQANIAIFSVG
                     RPSKSCILAQAGYLSAEDIDVLLSKGAVGDICSRFFTADGSVCDESLNDRTIGLELDE
                     LTQKESCILVAGGPKKIPGIAGALRGGFANMLVTDEITARTLVDSADGAL"
     gene            complement(1828..2139)
                     /locus_tag="PLM83_01870"
     CDS             complement(1828..2139)
                     /locus_tag="PLM83_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70189.1"
                     /translation="MAEEKPLGFFAAWKATDTWYSASEDEKREFDRKVEDIFSRAREK
                     GVKMHGVYDCCWSTEWKYFTFWEVPNLELLEEVMEELSEIGDINMYNAQRHFVGRKVR
                     D"
     gene            complement(2181..2678)
                     /locus_tag="PLM83_01875"
     CDS             complement(2181..2678)
                     /locus_tag="PLM83_01875"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HOK70190.1"
                     /translation="MHKVKVEELTKEAFAPYGQVVEMPEGAKKVSAVEDLWPGLVKWE
                     LDHETLEIAHGVCKKRPLIVEGLERHNTTYELLVPIKSDLIIPVASEDDRANKDAQPL
                     ARNVRGFLVKVGQAVFYDKGVWHLSPYPVEDEGPFFVVFKHKTAPDNIIMKELDEKVE
                     LEYEN"
     gene            complement(2783..3739)
                     /locus_tag="PLM83_01880"
                     /pseudo
     CDS             complement(2783..3739)
                     /locus_tag="PLM83_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(3836..4798)
                     /locus_tag="PLM83_01885"
     CDS             complement(3836..4798)
                     /locus_tag="PLM83_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70191.1"
                     /translation="MEKTQGFDYKTFIEKYGALAILALCMIVLTIISPYFLTALNLKT
                     IAIQTAVIAIIAIGQTFVIITGGIDLSVGANLALSNILTASMMTSGVPLWICLVTALA
                     FSAAFGYVNGLAITKMRVPPFIVTLGTMSIARGIALVITNGTPISRLPEALSWFGRGE
                     IVGLPVPVLIFIVAAIIFNWVLNHTLFGRYTYAVGSNMEATRLSGIDTGRTVRMVYLV
                     SGLLAGLAGIVLMGRLGLAHPTAGADYNMNSVAAAVIGGTSLTGGVGNIFGTMIGALI
                     MGVLDNGFVLLGLSSFYQEIAIGFVVIMAVYLDLLQQTKLRERR"
     gene            complement(4871..6358)
                     /locus_tag="PLM83_01890"
     CDS             complement(4871..6358)
                     /locus_tag="PLM83_01890"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013524742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOK70192.1"
                     /translation="MEYMLEMNHIDKRFPGVQALSDVTLKVKPGEVHVLLGENGAGKS
                     TLVKILAGVYRKDAGEIILGGQKVDYSSTNDASKLGISMIYQELNMIPDLTVAENIFI
                     NREHLKGWPKHIDWEKMNSEAAKALKKLNANISPTQKARRLTTAQKQMVEIARAISAD
                     SRIIIMDEPTASLTEKEIGELFRQIRLLSEENVSIIYISHRLNEVKEIGDRVTVLRDG
                     QYIGTRDIKEADTDDLIQMMVGREITQFFPKCVAKKGKEALRVENLSRKNVLHDISFS
                     VHEGEVLGFSGLIGSGRTELMRAIYGADGDVSGRVYVDGKEAKVSSPKLAVQNGIAFL
                     TEDRKNLGAILNFPIYQNITLASMDQFSRNSVIDHQAERSRSEELFSMFNIKAPSIDT
                     VTRSLSGGNQQKVLLARWFCRDAKIIILDEPTRGIDVGSKVEIYEFINELAGQGKAII
                     MVSSDLPEVLAMSDRIAVMREGRLTGILDREEATQEKIMKLATLG"
     gene            complement(6460..>7157)
                     /locus_tag="PLM83_01895"
     CDS             complement(6460..>7157)
                     /locus_tag="PLM83_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOK70193.1"
                     /translation="HAVEEALAYEEGGIDGIILENSGDYPFLAPGDVGFETVATMTAV
                     ACAVRDAIEIPFGVILNWNAAIPAMAVAKASGAAFIRVNEWVNAYVSNSGIIEGDSGN
                     ILRYRKNIGAENVKILADVNVKHGAHALVADRSISDQAWDAEYYDADVIIVTGTKTGS
                     AAPLEKIEAVRAGTSLPVITGSGLCVENAEDIMPLVDGAIVGTSLKENGVWWGRVQVD
                     RVKELVDKVSKFR"
     assembly_gap    7158..7167
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7320..8237)
                     /locus_tag="PLM83_01900"
     CDS             complement(7320..8237)
                     /locus_tag="PLM83_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOK70194.1"
                     /translation="MLDIIGVGDASVDLIIKVDHLPGHDEKVRGELLGKYPGGIIANF
                     CCAASRFGAKTGIVTTVGDDDFGRIAIRGLAEFGVDQGGLVVKEGEPTYFGVIQLDST
                     GEKALVVVETPLLVPSIEDVDVDYLNNARYVHLTSLDLELAKFVASSVNNDVRVSIDI
                     EPTADSGGLDFWEDVLRKLHIVSLNEAGLKALVRCEDICKGAKTLLDYGPQIVVITSG
                     ADGVDVFSGDDHFHVPAFKVPVVDTTGAGDCFNAVFLSGLVKGWDLVKAVNYANAAAA
                     LSIQHVGARTGLPTEAVTRSFFDSKTCNR"
     gene            complement(9210..10610)
                     /locus_tag="PLM83_01905"
     CDS             complement(9210..10610)
                     /locus_tag="PLM83_01905"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOK70195.1"
                     /translation="MKAWEYQLEMRRRAKPVDFTENPRDWSASFEAFEYRKGMLALLW
                     KSEVPGSRAPEALLHAAVQAWHNRGYDVSEAERIAERGLKVCAGGDHGKLERITAELM
                     HALENAPKDPTHPYWSYNQPETWEEIMACMPAMKESLEDHAEDVDDSDVERRILGGWT
                     GQIVGGSYGTAMEGYTGDAIREAYGDRLNYYVAEPETYNDDITYELAFLAAADEAGPG
                     LSSRDIANKWLELIPSGWSAEYFALENLRRGIYPPESGRFRNYYSEWIGAQMRTMVCG
                     LVAPGDPLRAARYAYLDSVVSHEKNGVYAGIHSAVLTSLAFVVDDTRVLLKHARDYVP
                     DGTEFASVLDDAMESVQANKDHIESYKRFEEKLKTYNWIHAYPNMVAVVHSLWYCRND
                     FDKALRILADCGADVDCNAGEVCCAMGAMNPDCIDPRWTEPFNNQLETYLPGFESMEI
                     TALARWTYEIYRKLQA"
     gene            10983..11543
                     /locus_tag="PLM83_01910"
     CDS             10983..11543
                     /locus_tag="PLM83_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK70196.1"
                     /translation="MIQTSAAALGLASALYLDLDAIRLALVQTSSNEIELLTTGVLYA
                     AEGDDETASLALTRARSMVPDEPELLAQICIGFAILDRLDKALKTAKMAVAADDGNAA
                     AHYALGMSLFGMEDYPDALSNFERAVSLRPDYYLAHAYNGKFLARMEKWPESRPIPFV
                     KTSLGQMWTAQSLCSDSGPQTSPLPV"
BASE COUNT         2668 a         3399 c         2583 g         3036 t
ORIGIN      
        1 gatttccgtc ttgtaaccgc cattcttggc catgtttgca gctgatcgca caacgcccac
       61 accatctccg cccacatcgt ccgcccttcc catgtagatg gagataaagt ctgctcccgc
      121 ttgaatagca aagaaggatt ggggaatcga cactatcccg gtcaggttga ccttaagtcc
      181 acggtcctgc agcacctgca tcgcagccaa tgactcctgg gagatgcaca tcttaacgat
      241 caaacgatca cccagaacac tagcaaactc ctcagcttgc gcaatgatgc cgtcacgctt
      301 gtcctcggta atctgcaaat ggaagggacc ttcaatgatc ttggcggcat cgctcaccgc
      361 ttctttccac ggtttcctca cttcggccac tatggtaggg ttcgtgatta tgccatgaac
      421 tccgtagtcc tttgctccga ttaggtcctt ctcatctatg ctagctatca atatcttcaa
      481 tgtagtagtc tcccttcagc tgccatgata cactcgcact taacaatgca ttccatcatt
      541 ctctggttct tgatggacag tgctgccttg cgcctaccaa aaccctatag tgctccatct
      601 gccgagtcca ccagcgttct ggcggttatc tcatctgtta caagcatgtt ggcgaagcct
      661 ccacggagcg ccccggcaat cccgggaatc tttttcggac cgcccgccac cagtatgcag
      721 ctctcctttt gggtcaactc gtcaagttca agcccaatag tcctgtcatt gagggactca
      781 tcgcaaacgg atccatctgc agtaaagaac ctggagcaaa tgtctccgac ggcgcccttg
      841 gataacagga cgtcgatatc ttccgcggaa agatagcccg cctgagccaa gatacaagac
      901 ttgcttggcc tccctacaga gaagatagca atatttgctt gtctaccctt ctcaagaatt
      961 cttctgagat tagggtcgcg ggctagggcg tctctgagtt ccctactttc aacaattgtt
     1021 ggagcagata agaagtacgg cggcgcatca aatgcagcag caactttggc cactatctca
     1081 tttccgtgga ttagtgcctc gcccttccca gaaagcgcac cctccagctg cacaatcttg
     1141 acgttttcgc gttttatggg caaaggtttg agcgctctgg ccatctccag catggtcgtg
     1201 cccccggtca ccccgatcac gtctccatct cggattatct cctttaggag acgtgcagca
     1261 gcctgcccga ggtgccatct aagaggcttg gggcttgatt cagagattgg gcataccact
     1321 actctcttca gaccgtacat ggattccaat tccctactga gcccatgttc ctcctggaat
     1381 tcatcgacga tagtcacctg aacaatgccg acttcccggg cccgagtcag cagccttgag
     1441 atgcggctcc gggacaggcc aactcgttcc gcgatctcct gctggttaag gtcatgttcg
     1501 tagtacatcc tcgctacccg aaccaaaagt tcgtgctcac tgctgggatt ctgttcaatt
     1561 tccacgacgt ttctcacttc ccaatggcat aaccaatact aacaagttat ctgtcaaccg
     1621 agacagacac gcgcatgctg gcacatctat gattgcctat gcacattctt gcactccaga
     1681 ggccttttct ccattgcaca agagactcct tcagccgcaa ggtgttaacc ttgggaagca
     1741 atgcagaccc gttgacagtg ctcatatcct ccttcgaaag cacgcaaaca acccggtacg
     1801 cgactgattc tgtcactaca gccaggacta atcccggacc ttgcgaccga caaaatgtct
     1861 ttgagcgttg tacatgttta tgtcgccgat ttcagagagt tcttccatta cttcctccaa
     1921 aagctccagg ttgggaacct cccagaaggt gaaatacttc cactctgtgg accaacaaca
     1981 gtcatacacc ccgtgcatct tgacgccctt ttcccgtgca cggctgaaga tgtcctctac
     2041 ttttctgtcg aattctcgct tttcatcctc cgatgcactg taccacgtgt cggtcgcttt
     2101 ccatgccgca aaaaatccca atggtttctc ttctgccatt acggtcaact ccagttcaaa
     2161 cgggctaagc cacacccagt ttaattttca tattcgagct cgaccttttc gtccagttcc
     2221 ttcatgataa tattgtcagg cgcggttttg tgtttgaaca caacgaagaa cgggccttca
     2281 tcctcaaccg gatagggcga cagatgccac acacccttat cgtaaaacac cgcctgccct
     2341 actttgacca agaagcctct tacattgcgc gccaagggct gagcatcctt gtttgccctg
     2401 tcgtcttctg acgctacagg aattatcagg tcgctcttga tcggaacgag caactcatag
     2461 gttgtgttgt gccgttccaa gccttccaca atgagtggac gcttcttgca tactccatgc
     2521 gctatttcga gggtttcgtg atcaagctcc catttgacca aaccgggcca gaggtcctcg
     2581 acggccgaca ctttttttgc gccttcaggc atttccacga cttgtccgta gggagcaaaa
     2641 gcttccttcg ttaattcctc gactttgact ttgtgcatga cttcttcaac tccaataccg
     2701 tcagttgctg ccttttttgc ggggttgcat ggcggaactg aacctgttcc gccatgcggc
     2761 cttcactcgt gtcggaacgc tcttatttcg ctgggtacaa caccttctga atctcaggcc
     2821 tattcatgtt ttccttggta actacggtaa tcggaacata aacatccttg ggcaccttct
     2881 tgccctgctt cgctgcgata atatattcaa cacccttgta gcccatttca aacggctgct
     2941 gcactacgag tccggctatc tcgcccttct tcagagcttc gatctcctcc gggcttgcat
     3001 cgtatgcaac aacctttacc ttgccagcaa gtcccctatt cctcagcgcc tgaatacagc
     3061 cgattgcggc gcggtcaaat gctgcaaata cgcccttaat gtcggggttg gcgttgagta
     3121 tcctctccat gtttgccatt gccactgcct gatcttctcc acgctggatc tcaagaagtt
     3181 ggaccttggg atacttcttt gccactgcta ccgctgcttc accgcgtatg tcgttgctag
     3241 ggacgcctgc agggcagttg agtatcacta ccttgccttc gccgcctatc tccttcatta
     3301 gcaattccat tgcgatgcgg ccgccttctc tgttgtctgt tccgacgtag ctcgtttttc
     3361 cgtcccagtt gagattggcg tcaatgctga ccagaggaat tccagccttc atcgcctgct
     3421 ttgccggtcc aaccagagca tctgaatcga ggggcgtcta caggatgcca tccactcttc
     3481 tgattatcag gttttctacg gcagagatct gctctgcaac gtcgttagtg cttttcgggc
     3541 ccgtgtaaat gagtttgccc ccaactttgg caagagcagc ctctgcgccc gccttaacgc
     3601 caagccagta ctcgctcgac gtagacatga cgaccagccc aattactaac tccttttcct
     3661 tggcggcaca aatcccaata ggacaagtta tcgcgaatac tagaaccagt gctaatgccg
     3721 acaacagtgt tcgtttcatc gtttcctctc ctatcgtttg atgttggcga attcgagctc
     3781 gtcgttgcct gtagatgtca tcattttggg gagcaatacc ggtcgcccgg tcccatcacc
     3841 tccgctctct cagcttcgtc tgctgcaata gatcgagata caccgccatg atcacaacaa
     3901 acccaatggc tatctcctga tagaatgaac tcaaccctag cagcacaaat ccgttgtcca
     3961 gcacacccat gatgagcgcc cctatcatgg tgccgaatat gttgccgacg cctccggtaa
     4021 ggctcgtgcc cccaataaca gcggctgcca ccgagttcat gttgtaatcc gcaccggccg
     4081 tggggtgcgc aagtccaagt ctgcccatga gtacgatgcc cgccagtccg gccaaaagcc
     4141 cagacacgag gtataccatc ctcacagtcc gtcccgtatc tattcccgat agcctcgtgg
     4201 cctccatatt gcttccaacc gcataggtat accttccaaa aagcgtatga ttgagaaccc
     4261 agttgaagat tatcgccgcg acaatgaata tcagaaccgg aacaggaaga ccgacgatct
     4321 ctccccgtcc aaaccaggag agcgcttccg gcagcctcga aatcggcgtt ccgttagtga
     4381 tgactagcgc gattcccctg gcaatcgaca tagttcccaa cgtcacgatg aacggaggaa
     4441 ccctcatttt cgttatggcc agaccattaa catatccaaa agcagcgcta aacgccaatg
     4501 ctgtaacaag acagatccaa agcggaacgc cggaagtcat catggacgcc gtcaggatat
     4561 ttgacaaagc caagttagcg ccgactgata ggtctatccc tccggtaatg atgacaaaag
     4621 tctgccctat cgcaataatg gcaataactg cggtctgtat agcgattgtc ttcaagttca
     4681 atgccgtcag gaaataagga ctgattatag tcaggacgat catgcatagc gcaaggattg
     4741 ccagagctcc atatttttct atgaatgtct tgtagtcgaa accctgtgtc ttctccattc
     4801 tctcatcagc ctccaaagga ctattcaacg agcattctca cagctaatgc cgaagtcagt
     4861 ttcttcatcg ctatcctaaa gtcgccagtt tcatgatctt ctcctgagtc gcctcttcgc
     4921 ggtcgagtat tcccgtcaat ctgccctcgc gcatcacagc aatcctatcg ctcattgcca
     4981 acacttccgg cagatccgac gagaccatga tgatcgcctt cccctgcccc gccagttcat
     5041 tgatgaactc atatatctcc accttggatc caacatcaat gccacgggtg ggctcgtcca
     5101 agatgattat cttggcatca cgacaaaacc acctggccaa aaggaccttt tgctggtttc
     5161 cgccactcag tgatcgagtg acagtatcta ttgaaggcgc tttgatgttg aacatggaga
     5221 aaagctcttc ggatctactt ctttctgcct gatggtcaat gactgaattt ctggagaact
     5281 gatccataga tgcgagggtg atgttctgat agatgggaaa attgaggatt gcgcccaggt
     5341 tctttcggtc ttcagtgaga aaggctattc cattctgaac agccagtttc ggcgaactga
     5401 cctttgcttc cttgccatcg acatacacac gtcccgaaac atctccgtca gccccgtaaa
     5461 tcgcccgcat gagctcggtc cttcctgagc cgatcaatcc ggagaagccc aggacttctc
     5521 cctcatgaac tgagaatgat atgtcatgca gcacgttctt cctgctcaag ttctctaccc
     5581 taagggcctc cttgcccttc tttgcaacgc atttcgggaa gaactgtgtg atctctctcc
     5641 caaccatcat ctgtatgaga tcatctgtat cagcttcttt gatgtcccga gttcctatat
     5701 attgcccatc cctgagaacg gtgactctat cgccgatctc cttcacttca ttaagcctgt
     5761 gagagatgta tattatcgaa acattttcct cgctgagaag ccgtatctgc ctgaacagct
     5821 caccaatttc cttttcggtc agcgacgctg tcggctcatc catgataatg atcctcgaat
     5881 ccgccgatat cgcgcgagca atctccacca tctgcttttg cgctgtagta agtctccgcg
     5941 ccttttgcgt gggtgatatg ttcgcattca gctttttcaa tgccttggcg gcttcagagt
     6001 tcatcttctc ccagtcgata tgtttaggcc agcccttgag gtgttcccgg tttatgaaga
     6061 tattctcggc caccgtaaga tctgggatca tgttgagctc ctggtatatc atgcttattc
     6121 ccagtttgga agcatcgtta gtggatgagt aatccacctt ttgtccaccc aatattatct
     6181 cgcccgcatc cttccgatag actcccgcga ggatcttgac aagagtggac tttccagctc
     6241 cgttttcgcc caaaagcacg tgcacctctc ctggtttcac ctttaaggtc acatctgata
     6301 gggcttggac accagggaat ctcttgtcta tgtggttcat ctctaacatg tattccattt
     6361 actttccctc gtttttcggt ctggtgacaa gacatttgct gaatatggag atcttcatct
     6421 cgtgcaccca tccctcaaaa tgatcgtggc ccatattgat caacgaaact tgcttacttt
     6481 atcgaccaat tccttgacgc gatccacctg aacacggccc caccaaacac cgttctcctt
     6541 caggctagta ccgacgatag caccgtctac gagcggcata atgtcctcgg cattctccac
     6601 acaaagaccg cttcctgtta tcacaggaag gctggttccc gcccgaaccg cttcgatctt
     6661 ctcgagaggc gcagccgatc cagttttggt gcctgtaacg atgatcacgt cagcgtcgta
     6721 gtattccgcg tcccaggcct ggtccgaaat ggaacgatct gcaaccaacg catgcgcgcc
     6781 atgcttcaca ttcacatctg caagtatctt gacgttctct gcgccgatat tctttctata
     6841 gcggaggatg ttcccactgt ccccttcgat tatcccagag ttggacacat aagcatttac
     6901 ccactcattg acgcggatga acgccgctcc ggaagctttt gctacggcca tagccggtat
     6961 ggcagcgttc caattgagaa taaccccgaa aggtatctct attgcatcac gcacagcaca
     7021 agctactgca gtcatcgttg ctacggtctc aaacccaaca tctccgggag ccaagaaagg
     7081 gtaatctccc gaattctcaa ggattatccc gtcgatccct ccttcttcgt aggctagcgc
     7141 ttcctctaca gcatggtnnn nnnnnnngag atcttcacct ttgtatctag gagaaccggg
     7201 aaaaggtaaa caatgaatag tgcctatcaa tggtttggga cgctcaaata tctctgatag
     7261 tgcattagcc tttggcggca cagcgctcat gcggaccaca tacctttcat cgatcagtgt
     7321 tatctgttac atgttttgct atcaaaaaag gaccttgtga cggcctctgt cggcaatcct
     7381 gttctcgccc ctacatgttg aatcgacagt gctgctgcgg catttgcata attgacagcc
     7441 ttcacaaggt cccaaccttt aactaaaccc gacaaaaata ctgcattgaa gcaatccccg
     7501 gcacctgtgg tgtcaactac cggtaccttg aaggcaggga catgaaaatg gtcatcccct
     7561 gaaaaaacat ctaccccgtc tgcgccactt gttatgacca caatttgggg gccatagtcc
     7621 agcaaagtct tagcgccctt acagatgtcc tcgcatctga ctaaagcctt caaccctgct
     7681 tcgttcagag aaacgatatg cagtttcctc aggacgtctt cccaaaaatc caacccacca
     7741 ctatcggcag taggctcaat atcgatggat actctcacgt cattgtttac gcttgatgcg
     7801 acgaatttgg caagctcaag atcgagggaa gtcaagtgca catagcgtgc attgttgagg
     7861 tagtccacat cgacatcctc aatagagggg acaagcagtg gggtttcaac aacaaccaac
     7921 gctttctccc ccgttgagtc caactggata acgccgaaat atgtaggctc accttccttg
     7981 actactagtc cgccctgatc cacaccaaat tccgccagac cgcggatggc aatacggcca
     8041 aagtcgtcgt caccaactgt tgtgactatc ccggtcttgg ctccaaacct agaagccgca
     8101 cagcagaaat tggcgattat gccgccggga tatttgccca gcaattcgcc ccttaccttt
     8161 tcatcatgcc caggcaagtg gtctaccttg ataatcaaat cgacacttgc atcgccgaca
     8221 ccaatgatat ccaacatttt gtgcccctct tgataagtat ttgtagttgc gcttcatttg
     8281 caccttcgtg cggttctagt tcttggcgca catttgtgcc cataggcgca catttgattc
     8341 ttgctagatt gtttcgccac gaaccgcaat actccttctc cgtttcaaaa agattctttg
     8401 tttgttgtac aagaaagcgg agctttttgt ggcgaaaatc cttgggcaac gttgaattta
     8461 ggaccatgcc cggggcttac ggacagtttt ggaagaggtt gaatatgatt tgcggggggt
     8521 atcgaggtgg ctgactgagc aagcaaagtg agtgttgaca agcgtgcgtt atgtaaaaca
     8581 tgcttctgcg ctattcaaga atcgtagtct tttacccata caattatgtt tatattatat
     8641 gcctctagct tcgaaaagta tctgagcatt cttaagcggc caaagtctta atctcagaca
     8701 aaagcactca tcagcgcatc tatcaaacca tagagcctgt tagctgtggc tgcagccttc
     8761 aaaagccgcc atcgcgacac tgataccacg tgcttcgcat ctcgcaatct actgccgaac
     8821 aatgttctca taggatgaac attcgggctg tcttggcgat acagtagcct tggctcgaaa
     8881 gcagaacctc cggagttgtc ggcggtgagc agctcccatc attggcttgt ggcgatttca
     8941 aggccaggag gctataagcc tttctcgccg gttacagatc gggttgtctt caatgtatat
     9001 tacctgctca aggcgaccta gattcgtgat cccaggatcg atcagctctg cgccgagcct
     9061 ctgctcggtc gaggcacggc gtacgctgtg gcgcctgcag ctgcttgagc attcatggca
     9121 gctaggaact gaaatgccaa tcctcatcca cagcatgagt gtaatctcat aacaccgttg
     9181 tcaggctatg tgcgcaagtt catcgcccct cacgcttgta gcttgcggta gatctcatat
     9241 gtccacctag ccagtgcggt gatctccatc gactcaaatc cgggcaagta agtctcgagc
     9301 tgattgttga agggttccgt ccatcgcgga tctatgcaat cgggattcat tgcccccatg
     9361 gcgcagcata cctcacccgc attgcagtca acgtctgccc cgcagtccgc caagatacga
     9421 agggccttat cgaagtcatt gcgacagtac caaagcgaat gcacgactgc gaccatgttg
     9481 ggataggcat ggatccaatt ataagtcttg agcttctctt caaacctctt gtacgattcg
     9541 atatggtctt tattcgcctg aacagactcc atggcgtcat ctagcaccga agcaaactct
     9601 gtcccatcag gcacgtaatc tcgggcgtgc ttgagaagca ctcttgtgtc atccacaaca
     9661 aaggccaatg acgtcaatac tgcgctgtga atccctgcat ataccccatt cttctcatgc
     9721 gacacaacac tgtcgaggta tgcataccgg gcagcgcgca acggatcccc cggcgccacg
     9781 agaccgcata ccatcgtgcg catctgagcg cctatccact cggagtagta attccgaaac
     9841 ctacccgatt ccggggggta tattcctctc ctcaagttct ccaacgcaaa gtactccgcc
     9901 gaccatccgg acgggataag ctcgagccac ttgtttgcta tgtcgcggga cgagaggcct
     9961 ggccctgcct catccgctgc ggcgaggaat gcaagctcat aagtgatgtc gtcattgtag
    10021 gtttcaggtt ctgcaacgta atagttcagc ctgtcgccat aggcttctct gatcgcatcg
    10081 ccagtgtagc cttccatggc cgttccgtag cttccgccga ctatctgccc cgtccagccg
    10141 cccaggatcc gcctctcaac gtccgaatca tccacatctt ccgcatggtc ttcgagactt
    10201 tccttcatcg caggcatgca cgccattatt tcctcccaag tttcgggctg attgtagctc
    10261 cagtaaggat gggtaggatc cttgggtgca ttttcaagag cgtgcatgag ctctgccgtg
    10321 attcgctcga gttttccgtg gtcgcccccg gcgcatactt tcaaaccgcg ttcggcaatt
    10381 ctttcagctt ctgatacatc gtagcccctg ttatgccacg cttgaaccgc cgcatgcaac
    10441 agcgcttcag gggctctcga gccgggtact tccgatttcc acaataacgc aagcatgcct
    10501 tttctgtatt cgaaagcctc gaagctagca gaccagtccc ttgggttctc cgtgaaatct
    10561 actggcttcg ctctcctcct catttcgagc tgatactccc atgccttcat agcaagccct
    10621 ccctatcgga ctactcgtag cccagtccct gcaaggccca aacctcattt tgggccccgc
    10681 atctgtattc ctcaaattca tcttatactc ctctgcccac gtcggcgcac tcggaatatt
    10741 cacggaactt tcacaatacc agaaaaggcc ccggcttgat gctccgctcc ttattgcata
    10801 tgccctatgc ttgcagtaca ccatagccat ggaccaagca tggatccacc cgtcctcagg
    10861 atgaattcgc ggccctgttc gaaagctcgt gccgttggta tcacttgtca tatcgatact
    10921 gccttgttga gtatctgctt tttcggggga ggaatggctc gtggcaaaga aagatagcat
    10981 cgatgatcca aacctccgca gccgcgctcg gcctggcttc cgctctctat ctcgacctcg
    11041 acgcaatccg gttggcgttg gtccaaacaa gctcaaacga aatcgaattg cttacaaccg
    11101 gagtcctata cgctgccgaa ggcgatgacg aaacagcctc ccttgcgctg actcgcgccc
    11161 gctctatggt ccccgatgag cctgaacttc tcgcccagat atgcatcggg ttcgcaatcc
    11221 tcgacaggtt ggacaaggcc ctcaaaactg caaagatggc agtggcggca gacgatggga
    11281 atgcagccgc ccactacgca ttgggcatga gcctcttcgg catggaagat tatccggacg
    11341 ctctgagcaa cttcgaaaga gctgtcagtc ttcgaccaga ctactacctt gcacatgctt
    11401 ataatgggaa attcctcgcc aggatggaaa agtggcctga gagcaggccc attccattcg
    11461 taaagacctc cctagggcaa atgtggaccg cgcaatcgct ctgttccgac tcggggccac
    11521 agacctcgcc gctaccagta tagatatgag cctggatgat gcctacgata tcggctgttc
    11581 gcatgttgac ccttgccttg attaatgatc cagacaacca cgactgcgca aatgacctta
    11641 ttgtttagtg cagactagaa ctacggggaa atgcagaatg gatttccctg aagttg
//
LOCUS       DAOSAI010000026        11638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3708_length_11638_cov_3.825434, whole genome shotgun sequence.
ACCESSION   DAOSAI010000026 DAOSAI010000000
VERSION     DAOSAI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11638
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3708_length_11638_cov_3.825434"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1524)
                     /gene="fliI"
                     /locus_tag="PLM83_01915"
     CDS             complement(181..1524)
                     /gene="fliI"
                     /locus_tag="PLM83_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOK70197.1"
                     /translation="MCADHIASDLARDIDFSRYHAKLKGLDTIKQRGRVAQVIGLVIE
                     SDGPHAQVGEICSIYPRNATLPVTAEVVGFRENKLLLMPLGEMAGIEPGSEVVAGGNF
                     LTVRVGDELLGRVLDGLGRPMDGLGPIHTVSEYPVSSSPPNPLLRPRISKLLPLGVRA
                     IDGLLTCGCGQRVGIFAGSGVGKSTLLGMIARNTRADVNVIALVGERGREVLEFIEGD
                     LGEEGLARSVVVVATSDTPALVRIKGAMVATAIAEYFRDQGLNVMFMMDSVTRFAMAQ
                     REVGLAIGEPPATKGYTPSVFALLPKLLERSGTSERGTITGLYTVLVDGDDLTEPIAD
                     AVRSILDGHIVLSRKLADANHYPAIDVLRSVSRVMDNIVSPGQRAAAGRFRDLLATYR
                     EAEDLINIGAYVAGSNPKIDESIAAIDGFNAYLRQGVGEKVSFDEAVEGLITVVG"
     gene            complement(1514..2251)
                     /locus_tag="PLM83_01920"
     CDS             complement(1514..2251)
                     /locus_tag="PLM83_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOK70198.1"
                     /translation="MIKRTLIDDVLVASPAFCVPTDRPELSFVASDEEEFVPEADGEA
                     AIEQAEARAKEIIARSRREADLILKDAKAEAESIAKRAEKEGYELGQAAGLAQVREAY
                     AEALNTIDSVADSIVAAADALVSGCECEVAKLAVAVAEKLVRKTIDEDPEAVLCMVRE
                     AVRRTEGARTVVMRVSPADLEVVARAHNELIGLSPDLRSLEIVEDARIERGGCVIEME
                     VGSVDARIDEQLREVERSFLEEAGHVR"
     gene            complement(2253..3266)
                     /gene="fliG"
                     /locus_tag="PLM83_01925"
     CDS             complement(2253..3266)
                     /gene="fliG"
                     /locus_tag="PLM83_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOK70199.1"
                     /translation="MNGARGAASGRRKAAIVLVSLDSETAASVLKHMKDDDIEQLTLE
                     IATLGKVTPEQQETALEEFYQLALAQEYIAQGGIDYAKQILEKALGTAKAMEIINRLS
                     ANLRVTPFAFARNVSPEQLTGFIEGEHPQTIALVVAHLRPDQAALVLSALPPDLQADV
                     ALRVAQMEGARPEVIKEVEEVLERKLTTFGTQEFQAAGGVKSLVEVLQNVDRSTEKVI
                     LERLEEENPEIAEEIKNLMFVFEDIVFMEDRYVQMIIREIDSKDLALALKAASDEVKD
                     KIMRNMSERAATMLQEELEYLGPVRLRQVEEAQMKIVAVIRKMEEAGQIVAIREKGDE
                     VIV"
     gene            complement(3286..4899)
                     /gene="fliF"
                     /locus_tag="PLM83_01930"
     CDS             complement(3286..4899)
                     /gene="fliF"
                     /locus_tag="PLM83_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOK70200.1"
                     /translation="MGIPDAGQDDIAQTEKSGLMSGLTKKQKIIVAAIVGGVFIVLIL
                     LIVSSSSKDYAVLYSGLSVEDAGRIVEHLKESGVKYRLTSGGHTIEVPEDRVYELRME
                     LAAKGFPQGAGAGFELFDKTSLGATEFTQKVNYTRALQNELARTIMTLTPVDQARVHI
                     VIPEERLFSEQQKPTTASIVLQMKPGAYLLDAQVRGVVHLASSAVQGLTPENVTVLDT
                     DGNLLWRASTAESTAGPMTTAQLEAKMGFEQEMQRRIETMLDRAFGLGKSIVRVYADI
                     NFDSAERASEIYQPLEGGLGIPVAVQTTQEERVGDSDAARGYAGTPSNVEGYPVLSGT
                     GSGESRYMRSESTTNYEVSKVVQKEIIAPGKVERVSVAVMLDRVMPMDRMEEMRQVIS
                     AAVGIQPERGDQIAITTMEFARAASEDEMFASAESLAKADKQAFIMRLVKSLTPLILG
                     LLVLLFAMSFLRVFREQQEYATEPAIVGRKVEEVLAEEASPEPLSPAALVALAEEKQK
                     AEMRQGVEALARKKPGDVARILETWMSED"
     gene            complement(4900..5202)
                     /gene="fliE"
                     /locus_tag="PLM83_01935"
     CDS             complement(4900..5202)
                     /gene="fliE"
                     /locus_tag="PLM83_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOK70201.1"
                     /translation="MSRIDGIGDSMALLGTPSVSSNDGTDLVRAFGDVLEQALDTVND
                     MQSRADRLTEKLAAGEISDIHQVMIAVEQVNLAIQLTMQVRNKIIESYQEVMRMQI"
     gene            complement(5432..5821)
                     /gene="flgC"
                     /locus_tag="PLM83_01940"
     CDS             complement(5432..5821)
                     /gene="flgC"
                     /locus_tag="PLM83_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOK70202.1"
                     /translation="MAPMSSFGVSASGMSAERLRLDAISNNLANINTTRTPEGGPYRR
                     IMVMLESSAGGVRVAGIERDSSPPMMVYDPGHPDAGADGCVAMPNVNPVLEMVDMITA
                     TRAYEANVAAFNAAKSMAAKALEIGKI"
     gene            complement(5825..6262)
                     /gene="flgB"
                     /locus_tag="PLM83_01945"
     CDS             complement(5825..6262)
                     /gene="flgB"
                     /locus_tag="PLM83_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOK70203.1"
                     /translation="MEFFESPTIKVLGAALDISSARHTAISRNVANANTPGYKAVDMD
                     FSETLKRSMAGAGAPRSVGVKATRPGHFGSRSHAATGAAGLSPVAVSSDPDGVSMRAD
                     GNSVDIDLEMAKMAENVILYNTVSHILASKFGMLRYVISEGRR"
     gene            complement(6469..8280)
                     /locus_tag="PLM83_01950"
     CDS             complement(6469..8280)
                     /locus_tag="PLM83_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK70204.1"
                     /translation="MGSAHARLWVPGAMFAALLAARFVYGWIVPTAGVWWVAAIDASL
                     VFCSAFIAAYYARALKSQLDAVEAVSDRAGRLELVARELVDNEAVMREDLVRGQRELN
                     ERILRLSSLLDATRELVGARNLEDALQHVADMTCKVMGADKATISVTMADEALTVVST
                     GTNCSDSLLFRSSSEIVLFGDAAGELVVEFANISPGNADAGSFLSIISAFASAAIENA
                     MLYRNLTEANEKLEVSRRYAERLIEEAPLGMVVIDDECRVLTWNRALAEITNVCRGAA
                     TGKDLHDIITGSGSERLFALIDKASAEGDSTVAENIALHIEHGDDHIFRIIVSPVERL
                     LGDNPGFIIMAEDVTEKVRLGEQLKQVERLKTLGEFAAGVVHEINNPIGIICACAEVM
                     EKKLSRHGPKMAECVKTAQIIQDEAIRCSSIVKNLLGFARQGEINPEEVDVEEVLRQV
                     VEFIKGYAAASNVEMDLKVSGDIPLIIGDVLQLEQVFLNLSINAIEAMAGGGRLEVSA
                     CCAGDEVRISFSDSGPGIEGVAAQKLFNPFFTTKSTGTGLGLSVSLGIVERHGGRIEV
                     GDSDAGGACFTVVLPLTIDAWEDHLPRSIGKHLHAGE"
     gene            complement(8360..10045)
                     /locus_tag="PLM83_01955"
     CDS             complement(8360..10045)
                     /locus_tag="PLM83_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOK70205.1"
                     /translation="MEQCLGSLQMGDNVVETGMRDDVKASARRVLIVDDETNMQVVLS
                     AAFEDAGFATGTASTGEEAIRALTQTDYDLMLLDLKLPDMTGIDVFRHAREASPGTIV
                     IMITAYSSVETAIEAMKMGAYDYITKPFNLEKLLMIAGNAIDARRCARIDSGSSAGLL
                     PSARLVRGGLERNAMRGDTAKAGSAVAFHGGKAAGTLSQADWALSGIELVAEDEDFDG
                     VVGVTPQMRQLMEMSRDIAPTNATVLIYGESGTGKELVADYIHKKSLRAGRPFIKVNC
                     AAIPEPLLESELFGHEKGAFTHAISRRLGRFELADKGSILLDEVGEMSNAMQAKLLRV
                     LQEKEFERVGGTETIKVDVRVIAATNQNLKEAVLRGDFREDLYYRLSVVPLFLPPLRD
                     RKRDIPLLCARFIDKYNKEFGREVSGVSPKVLEMFAEYDWPGNIRELQNAMERAVLLS
                     RSDVLTPDDFHLGMDPAEIISLGTGVTCRSCANSRLAGRNWDGSGADMVSDGADGYIE
                     ILDENGSPLSLEEMEKRHIKAVLDQFNWNKTQAAQALNITRRTLLNKINKYDL"
     gene            complement(10193..10690)
                     /locus_tag="PLM83_01960"
     CDS             complement(10193..10690)
                     /locus_tag="PLM83_01960"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOK70206.1"
                     /translation="MAHELFSDIASLEERIHSCREEQLALYKRLLELSHAQRGAIARG
                     DTDALLWATEEKGRLIERIDAIDIRLAGLIDDASILAGKEGLSHLCPYKEPGIDSACP
                     LNVKIAQIIKTILETDRENQAILEEAIGAAGDEIKQLDSGGKAVKAYWRRSPESPAGS
                     VDDVF"
     gene            complement(10690..11160)
                     /gene="fliS"
                     /locus_tag="PLM83_01965"
     CDS             complement(10690..11160)
                     /gene="fliS"
                     /locus_tag="PLM83_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOK70207.1"
                     /translation="MMKCRAANGRDGGSMMTSAYNRYMEVQVQTAPPENLVLMLYDGA
                     IRFATQAKTDFAEGNREAAHNNLTRAQDIMGELMGSLNMEIGEVAGNLFSIYEYMQHR
                     LIEANIRQSVECIDEVLKMLGELRQTWAEAIRQYRGQSAVSADSVGGGERGEVG"
     gene            complement(11157..>11638)
                     /gene="fliD"
                     /locus_tag="PLM83_01970"
     CDS             complement(11157..>11638)
                     /gene="fliD"
                     /locus_tag="PLM83_01970"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOK70208.1"
                     /translation="SAVRGIQTSLRRLTMASVEGQPKGFNSLLAIGISTGAPGRKVGS
                     STGTLTIDEAKLKEALTSDPQSVLRLFNEAEQGIAVQYEKYIDELVKSTSGLISQTQK
                     VLKEQTKDIDRRIEEMEARLEKRKESLIRQYTQMEKALAAMYQQQMWMDAQFKSMGV"
BASE COUNT         2571 a         3706 c         2676 g         2685 t
ORIGIN      
        1 gcctcagcca tctcggactg cccttgcccg aaattccact ccatatccgc caatacctgg
       61 cgggcctcga gctcgcgcga ttgggccttg gcaaattcct gctttgcctg gtcttccagg
      121 cgtcttctat gatccaggac cggttgcagt ttgaaagtaa aagtgtccat gccgatcccc
      181 ctatcccaca actgtaatca aaccttctac agcctcgtcg aacgaaacct tctcccccac
      241 gccctgcctg agataggcat tgaacccgtc tattgcggca atcgactcat ctatcttcgg
      301 gttcgatcct gccacatagg ccccgatatt tatcaaatcc tccgcctctc ggtatgtggc
      361 tagtagatcg cggaaccttc cagcagccgc ccgctgccca ggagagacaa tattgtccat
      421 tactcggctg acgctccgga gcacatcgat ggccgggtag tggttggcgt cagcaagttt
      481 gcgcgaaagg actatgtgtc cgtcaagaat gcttcgaact gcgtcggcaa tcggttcagt
      541 caggtcgtca ccgtccacga gcaccgtgta aagaccggtt atcgtccccc gctcgctagt
      601 ccccgagcgt tcaagcaatt ttggaagcag cgcgaagaca gaaggagtat atcctttggt
      661 cgccggcggc tcgccgatgg ccagcccaac ctcgcgctga gccatggcaa aacgggttac
      721 cgaatccatc atgaacatca cattgagccc ctggtcacgg aagtattcgg cgatcgcggt
      781 agccaccatg gctcccttta ttctgaccag cgctggagtg tccgaagttg ccacaaccac
      841 cacagacctg gccaatcctt cttccccgag gtctccttct ataaactcga ggacctcgcg
      901 tccgcgctct cccaccagcg caatcacgtt tacatcggcc cttgtattcc tggcgatcat
      961 gcccaacaga gtactcttgc ccaccccgct ccctgcgaag attcccacac gctgcccgca
     1021 gccgcacgtg agcaatccgt ctatagctcg aaccccgagg ggcagcagct tcgaaatcct
     1081 cggcctcaaa aggggattcg gtggggaact cgacacaggg tattcagaca cagtgtggat
     1141 cggccccaat ccgtccatgg gcctcccaag cccgtccaga accctcccta gaagctcatc
     1201 acccacgcgc acagtcagga aattgccccc cgccacgacc tcgcttcccg gctcaattcc
     1261 agccatctct cccagcggca tcaagagaag cttgttctca cggaacccca ccacctcagc
     1321 cgtcacaggc aaagtggcgt ttcgagggta tatggagcaa atctctccga cctgcgcatg
     1381 gggcccgtct gattcgatca caaggccaat aacctgtgcc actcttccac gctgcttgat
     1441 agtgtcaagg cctttaagct ttgcatggta tctactaaaa tctatatccc tggccagatc
     1501 ggacgcaatg tgatcagcgc acatgtcctg cttcctccaa gaaggatcgc tccacctcgc
     1561 ggagctgctc atctattctg gcatccaccg acccaacttc catttcaatc acacagccac
     1621 cacgctcgat cctggcgtct tcaacaatct ccaggctgcg aagatcgggg gaaagtccga
     1681 tcaactcgtt atgtgcccgg gcaaccacct caagatcagc aggtgaaacc ctcataacca
     1741 ctgtcctggc cccttcagtt cttcgcaccg cctcacggac catgcaaaga acagcttccg
     1801 gatcctcatc tatcgttttg cggacaagct tctcagccac tgccacagcg agcttcgcca
     1861 cctcgcactc gcatccgctc accaaggcgt cggccgccgc tactattgaa tcggcaacag
     1921 aatctatcgt gttcagagcc tcagcatagg cctcccggac ctgggcaagg cccgcagcct
     1981 gccccaactc gtatccttcc ttctcagccc gctttgcaat actctccgct tcagccttgg
     2041 catccttaag aatcaggtcc gcctcccgcc gagatctggc gatgatctct ttggcccggg
     2101 cctcagcctg ctctatcgca gcctctccgt ccgcctcggg cacgaattct tcctcatccg
     2161 aggcgacaaa ggatagttca ggcctgtcag ttggcacgca aaatgcggga gaagcgacaa
     2221 gaacgtcgtc tatgagtgtc cgctttatca ccttagacaa tcacttcgtc ccccttctcg
     2281 cgtatggcga cgatctgccc ggcctcttcc atctttctta tgactgcgac aatcttcatc
     2341 tgcgcctcct caacttggcg gagccttaca ggaccgagat actccaactc ctcttggagc
     2401 attgttgctg ccctttccga catgttccgc atgatcttgt ccttcacctc atccgaagca
     2461 gctttcaaag ccaaagcaag gtccttggag tctatctccc tgatgatcat ctgcacgtag
     2521 cggtcttcca tgaacacgat gtcctcgaag acgaacatga gattcttgat ctcttcggca
     2581 atctccgggt tctcctcctc gagcctttcg agaatgacct tttcagtgga gcggtcgacg
     2641 ttctgcaaaa cctccaccag cgacttgacg ccgccggcag cctgaaactc ctgagttccg
     2701 aaggtagtca gtttccgttc gagcacttcc tcgacttcct tgatcacctc gggccttgcg
     2761 ccctccatct gggccacccg aagcgccaca tccgcctgga gatccggggg aagcgccgac
     2821 agaaccagcg ccgcctgatc tggtctgagg tgggcaacga ccaaggctat agtctgcggg
     2881 tgctcgccct ctataaagcc ggtgagctgc tcagggctga cattgcgcgc aaaggcaaaa
     2941 ggcgtcacgc gcagattggc cgaaagcctg ttgatgatct ccatggcctt agccgtaccc
     3001 aacgccttct ccagaatctg tttcgcgtaa tctattccgc cctgggctat gtactcctga
     3061 gcgagagcca gctggtaaaa ctcctccagg gcggtctcct gctgttcagg cgtgaccttg
     3121 ccaagcgtcg ctatctccag cgtcagttgc tcaatatcat cgtccttcat gtgcttgaga
     3181 acggatgcgg cagtctcaga atcgagagac accaggacta tagctgcctt ccgccttccg
     3241 cttgccgcgc cccttgcgcc gttcatttcg acctgcaccc gcctctcaat cttccgacat
     3301 ccacgtctca agaatccttg ccacatcgcc tggtttcttc cgggctagcg cctcaacgcc
     3361 ttgtctcatt tctgccttct gtttttcctc agccaaagcg accaatgccg ccgggctcag
     3421 aggttccggg gaagcctctt ccgcgagcac ttcttcgact tttctaccta caatggcagg
     3481 ctcggttgca tattcctgct gctcacggaa gactcgaaga aaagacatgg caaacaggag
     3541 cacgagcaat ccgaggatga gaggggtaag cgacttgaca agacgcataa taaaggcctg
     3601 cttatccgct ttcgcaagac tctccgctga tgcaaacatc tcgtcctcgc tcgcggcccg
     3661 cgcaaattcc atggtggtaa tggctatctg gtccccccgc tccggctgaa tacctaccgc
     3721 agcgcttatc acttgccgca tctcctccat gcggtccatc ggcatcaccc gatcgagcat
     3781 cacagcgaca gagaccctct ccactttgcc cggagcgatg atctccttct gaacaacctt
     3841 ggacacctcg taattagtcg tgctttcgct tctcatatac ctcgattcac cgctgcccgt
     3901 ccccgaaaga accggataac cttcgacatt cgacggcgtt cccgcatagc cccgggcggc
     3961 atccgaatcg ccaacccgtt cttcctgagt cgtctggaca gctaccggta tccccaggcc
     4021 gccttctaaa ggctggtaga tctcgcttgc ccgctctgcg ctgtcgaagt tgatgtcagc
     4081 gtatacccgc actatcgact tgccaagtcc gaaagcacgg tcgagcatcg tctcgatccg
     4141 tctctgcatc tcctgttcaa accccatctt ggcttcaagc tgcgccgtag tcattgggcc
     4201 ggcagtcgat tccgcagtac tggctcgcca aagcaaattc ccgtctgtat cgagcacagt
     4261 cacattctca ggggtaaggc cctgcacagc actggacgcc agatggacca caccccgcac
     4321 ctgagcatcg aggagatatg cgccgggctt catctgaagg actatcgatg ctgtcgtggg
     4381 cttttgctgc tccgaaaaga gcctttcctc ggggatcaca atatggaccc tcgcctgatc
     4441 tacaggggta agagtcataa tcgtcctcgc cagctcattt tggagggccc gggtgtagtt
     4501 taccttctgc gtaaactcag tcgcccccag actcgtctta tcgaacagct caaatccggc
     4561 gccggcgccc tgtgggaaac ccttcgccgc caactccatc ctcagttcgt agacacggtc
     4621 ctctggaacc tcgatagtgt gccccccaga agtcaacctg tacttcacgc ccgattcctt
     4681 tagatgctcg acgatcctgc cggcgtcctc aacagacaag ccggaataaa gtacggcgta
     4741 gtcctttgag gagctggata caatgagaag aatcaggacg atgaatactc ccccgacaat
     4801 tgccgcaact atgatttttt gcttctttgt caaaccggac atgagcccag acttctcagt
     4861 ctgagcgata tcgtcctgac ctgcatcggg aatacccatt tatatctgca tcctcattac
     4921 ttcctgatat gattcgatga tcttgttcct tacctgcatg gtaagttgga tagccaagtt
     4981 cacctgctcc accgcgatca tcacctgatg gatatcgctg atttcgccgg cggcaagctt
     5041 ctcagtgagc ctgtcggcac gggactgcat gtcattgacg gtgtcgagcg cctgctcgag
     5101 cacgtcgcca aaagcacgaa ccaaatctgt cccatcattg gacgacacgg acggcgtccc
     5161 aagcagcgcc atgctgtcgc ctatcccatc gatcctcgac atccgcatgc acctccatgc
     5221 gcctgcttca agattccctt tccatctgag caaccctcag tcaaactctg tgccgcctgt
     5281 attttgcatt ttgcgaaatc cagagcagag cttttccaca ctactcaaga atgttctcaa
     5341 aatcagtctg gcagcttcca gtatctcttc gcgccatcca aaatccacgt tcaagctccc
     5401 ggatagcgtt caggcactcg tcgaaaacct actatatctt ccctatctcg agcgctttcg
     5461 cagccataga cttggcagca ttgaacgcag caacattcgc ctcgtatgcc cgggtagccg
     5521 taatcatgtc caccatctcc agcaccggat tgacattagg catggccaca cagccatcgg
     5581 ccccggcatc tggatggccc ggatcatata ccatcatcgg cggcgaactg tcccgttcta
     5641 ttcccgccac gcggactccg ccagcagacg attctagcat caccatgatc cgcctatatg
     5701 gcccgccctc cggcgtgcgc gtggtattga tattcgcaag attgtttgaa atagcgtcaa
     5761 gacgcagcct ttcagcggac attcccgacg cgctcacccc gaaactcgac atcggcgcca
     5821 ttcattaccg cctcccctcg ctgataacat acctaagcat cccgaacttg ctggccaaaa
     5881 tgtgcgacac agtgttgtac aagatcacat tctccgccat cttagccatc tcgagatcga
     5941 tatccacact gtttccatcc gccctcatgg aaaccccgtc aggatcagac gacactgcca
     6001 ccggcgaaag ccccgccgcg ccagtcgccg catgagaacg agatccgaag tgccccggcc
     6061 gcgtagcctt taccccaaca gaacgagggg cgcccgcgcc agccatggac cgcttcaatg
     6121 tctcgctgaa atccatgtcg accgccttgt atcccggggt gttggcgttc gccacattgc
     6181 gcgagatagc cgtgtgcctg gcggaggaga tatccagggc cgctccgagc acttttatag
     6241 tcggactttc aaagaattcc acggaccgaa gcctcctttc gaaaaggaaa tacgaaatcc
     6301 aataaatata gatgcaataa tgatgccagc gaaacagaac aaaagagcct ttccgcaaac
     6361 ggacgtggaa aggctatgta agctggcaat atgttcctca tccagtttca agccgaaagt
     6421 ggcgccagcg caaacgggga cccgctcatt ttctgaggtg agcaattttt actcacctgc
     6481 atgcaagtgt ttccctatgc tccggggcaa atggtcctcc caagcatcta tagtgagggg
     6541 gaggaccaca gtgaaacacg cgccgccagc atcagaatct cccacttcga tcctgccccc
     6601 gtggcgctcc acgataccaa ggctcaccga caatcccaga cctgtgccag tcgacttcgt
     6661 agtgaaaaaa ggattgaaca gtttctgggc agctacgccc tcaattcccg gtcctgaatc
     6721 agaaaaggag atccttacct catcccccgc gcagcaggca gatacctcca accggccacc
     6781 acctgccatc gcctctatgg cgtttatgga cagattaaga aagacctgct caagctggag
     6841 cacatcaccg attatcagcg ggatatcccc gcttactttc aaatccatct ctacattgga
     6901 agcagccgca tagcccttga tgaactcgac aacctgccta agtacctctt cgacgtctac
     6961 ctcttcaggg ttaatctcgc cctgtctggc aaagccgagg aggttcttga ctatggaaga
     7021 acaccttata gcctcatcct gtataatctg ggcagtcttt acacactcag ccatcttggg
     7081 cccgtggcgg gagagcttct tctccatcac ctcagcacac gcacagatta tgcctatagg
     7141 gttgtttatc tcgtgcacta ccccggcggc aaactcgcct aaagtcttca atctctccac
     7201 ctgcttgagc tgctctccga ggcgaacctt ttccgtcaca tcctcggcca tgattatgaa
     7261 accggggttg tcgccgagaa gacgttcgac cggagagaca ataatccgga aaatgtgatc
     7321 gtccccatgt tcaatatgga gggcaatgtt ctccgcaaca gtggaatccc cctctgcaga
     7381 tgccttgtca atcagggcga atagtcgctc actgccggat cccgtgatga tatcgtgaag
     7441 atccttccct gtggcggccc cccgacaaac attagtaatc tctgccaacg ccctattcca
     7501 agtgagcacg cggcattcat catctatcac caccatgcct aaaggggcct cctcaatcag
     7561 tctttccgca tatcttcgcg agacctcgag tttctcattg gcctcagtca gattccgata
     7621 aagcatagca ttttcaatcg ccgcactagc aaaagcagat atgatagaca agaagctgcc
     7681 tgcgtcggca ttgcccggac tgatgttggc gaactccact accagttcgc ctgctgcatc
     7741 accaaacagg acaatctccg aagaagagcg aaagagaagg gaatcgctgc agtttgtgcc
     7801 cgtggatacg accgtaaggg cttcatctgc catagtcaca cttatcgtcg ccttgtctgc
     7861 acccattacc ttacacgtca tatcggccac atgctggaga gcatcctcca gattccttgc
     7921 gcccacaagc tcccttgttg catcgagaag gctggagagc ctgagaatcc tctcattcag
     7981 ctcacgctgg ccgcggacga gatcctccct cattacagct tcattatcca cgagttccct
     8041 cgccacaagc tcgaggcgac ccgcgcgatc tgaaacggcc tccaccgcat ccaattgaga
     8101 cttgagagcc cttgcgtagt aagccgcgat aaatgctgag cagaacacga ggctggcatc
     8161 gattgccgcc acccaccaaa ccccggcagt cggaacaatc cagccataaa caaatctggc
     8221 cgccaaaaga gcggcaaaca tcgctccagg cacccatagt ctagcgtgtg cgcttcccat
     8281 ggctgtcaca ccctcattaa aaatacctct catcgacgct agacagcagt gactgtgtcc
     8341 gacgcgcctg ctcgagaggc tacaggtcat acttgtttat cttgttgagc agagtccgcc
     8401 tggtaatatt gagggcttgg gctgcctgag tcttattcca gttgaactga tccaaaaccg
     8461 ccttgatgtg tcgcttctcc atctcctcca aagacagcgg actcccgttt tcatccaaaa
     8521 tctcgatgta gccgtctgca ccatcggaca ccatatccgc cccactgcca tcccagtttc
     8581 gcccggccag cctcgagttg gcgcagctgc ggcaggtgac ccccgtcccc agggatatga
     8641 tttcagccgg gtccatccca aggtgaaaat catcaggcgt caacacatca cttcttgaaa
     8701 gaagaaccgc ccgttccatc gcgttctgca gctcgcgaat attccccggc caatcatatt
     8761 cggcaaacat ctccagcacc ttaggggaaa cacccgagac ctccctgccg aactccttgt
     8821 tgtatttgtc gataaacctc gcgcacagca gtggaatatc acgcttccga tctcgcagag
     8881 ggggaagaaa aagaggcaca accgacaatc gatagtaaag atcttcacga aaatcgcccc
     8941 tcagcaccgc ctctttaagg ttctgattgg tagctgcaat caccctcaca tctaccttta
     9001 tcgtctccgt gcccccgact cgctcgaact ccttctcctg gaggaccctg aggagcttgg
     9061 cctgcatagc attggacatc tcgccgactt catcgagtaa tatggaaccc ttgtcggcaa
     9121 gctcaaacct gccaagccgt ctggagattg catgggtaaa agcacccttc tcatgcccga
     9181 aaagctccga ttcgaggagg ggttcgggaa ttgcggcgca gttcaccttt atgaagggtc
     9241 tgcccgccct gaggctcttc ttgtgaatgt aatccgcaac aagctctttc cctgttccac
     9301 tttcgccgta tataagaacg gtggcattgg tgggtgctat atcccgcgac atctccatca
     9361 actgcctcat ctgcggcgta acgcccacaa ccccgtcgaa gtcctcgtct tcggctacaa
     9421 gttcgatgcc tgagagagcc cagtcggctt ggctgagggt ccctgcagcc ttgcctccgt
     9481 gaaaagccac tgcactaccg gcctttgcgg tgtccccgcg catcgcgttc cgttctaggc
     9541 caccacgcac caaacgtgca gacggcagta ggccagccga agagcccgag tctattcttg
     9601 cacatcgcct tgcatcaatg gcgtttcccg cgatcatgag gagtttttcc agattgaatg
     9661 gcttggttat gtaatcataa gcgcccatct tcatagcctc tatcgcagtc tccactgaag
     9721 aataggcggt tatcattatc acgattgtac ccgggctcgc ctcccgcgcg tgcctgaaca
     9781 cgtcgattcc cgtcatatca ggtagcttga gatcgagcaa catgaggtca taatctgtct
     9841 gagttagggc acgaatagcc tcctccccag tcgatgccgt gcctgtcgca aaacccgcat
     9901 cttcgaaagc agccgagaga actacctgca tatttgtctc atcatcaaca ataagtacgc
     9961 gcctggcaga cgccttcaca tcgtccctca tacccgtttc cacaacattg tcccccatct
    10021 gaagagatcc gaggcactgt tccatataat ggaacaacct acaaccccat tacaattctt
    10081 cagatagtta catatatcct tcttatttca gcaaaaacag catgggcacc aagggatata
    10141 tgcgagaccg tgcaaagaac ggtgagaaag tcggcagcct aacgtggaaa attcagaaga
    10201 cgtcgtcgac gctgccagca ggagattcgg gagaacgtct ccagtaggct ttgaccgctt
    10261 tgccgcctga atcgagttgc tttatctcat cgccggccgc tccgatcgcc tcctcgagaa
    10321 tggcctggtt ctccctatct gtttctagga tcgtcttgat gatctgggct atcttcacgt
    10381 tgagcggaca ggcagaatct atgcccggct ccttgtatgg gcaaagatgt gataggccct
    10441 cttttccggc caggatcgag gcatcatcga ttaggcccgc cagcctgatg tctatcgcat
    10501 cgatcctctc gatcagcctc cctttctcct cagtcgccca gagaagagca tcagtgtcac
    10561 ctctggcaat agccccgcgc tgggcatggg aaagctcgag cagtcgcttg tagagagcga
    10621 gctgctcttc gcggcatgaa tgtatacgtt cctctaatga agcgatatca gaaaagagtt
    10681 catgcgccat cagcctacct ccccccgttc gcctccaccc acagaatctg cgcttacggc
    10741 agattgtccc cggtactgcc tgatcgcttc cgcccacgtc tggcggagtt caccaagcat
    10801 cttgaggacc tcatctatgc actcaacaga ctgtcttatg ttggcctcaa tcagtcggtg
    10861 ctgcatgtac tcgtaaatgc taaaaaggtt cccagctact tcgccgatct ccatattgag
    10921 tgagcccatg agctcgccca ttatgtcctg ggcccgagtg aggttattgt gggcagcctc
    10981 cctattcccc tcagcaaaat cggtcttggc ctgagttgcg aaccttatgg caccatcata
    11041 tagcatgagg acgaggtttt caggtggcgc cgtctgaacc tggacttcca tatatctgtt
    11101 ataggcactg gtcatcatcg aaccgccatc ccttccattg gccgccctgc atttcatcat
    11161 acccccatcg acttaaactg cgcatccatc cacatctgct gctggtacat ggccgcaagc
    11221 gccttttcca tttgagtata ctgtctgatc aacgattctt ttcgtttttc gaggcgagcc
    11281 tccatctctt ctatccggcg atctatgtct tttgtctgtt ccttcaaaac cttttgagtc
    11341 tgagatatca gccccgatgt gctcttcaca agttcgtcaa tgtacttttc atactgaact
    11401 gctatgccct gttccgcctc gttgaaaagc ctgaggacag attgtgggtc acttgttagg
    11461 gcttccttca gcttcgcctc atcgatggtg agcgtaccgg tgctggagcc aaccttcctc
    11521 cccggagcgc ccgtggatat gcctatcgcc agaagcgaat tgaaaccctt gggctgccct
    11581 tccacagagg ccattgtaag acggcggaga gatgtctgaa tcccgcgcac cgccgaat
//
LOCUS       DAOSAI010000027        11436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3786_length_11436_cov_3.920130, whole genome shotgun sequence.
ACCESSION   DAOSAI010000027 DAOSAI010000000
VERSION     DAOSAI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11436
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3786_length_11436_cov_3.920130"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..1187)
                     /locus_tag="PLM83_01975"
                     /pseudo
     CDS             complement(270..1187)
                     /locus_tag="PLM83_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1633..2979)
                     /locus_tag="PLM83_01980"
     CDS             complement(1633..2979)
                     /locus_tag="PLM83_01980"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOK70209.1"
                     /translation="MKELELCDSLVERARKMGADDAEVFYIKSRQLGAVFEKNDLQVP
                     KGDDYDGLGIRVLLYGKGGAKQGFAATNMLSPDSLEETLLAAIAIANASPDDPNHWMP
                     EPSTIEPVPGRYDESAETMSLEDAVEQGLALVNSARAFDPRVTLDSAQYMVDITEKAI
                     VNSKGIRLVEKDSVFTCLAMGFARDEKEVSSFDLEVAVTCRKDEIDPTAIGRKLAEKV
                     VASLGATTVPSFKGDVIITPYAGYDLIAEPISFSCNAQNVQNGRSKWAGMLGTEVMSP
                     VISVTDDPKLPKAAGSTSFDREGVPPAPLQLVSEGTLNHYLYNCYTAKKDGVASNGRA
                     AGSDHTVPGISTTNFVVASGKHSLDEMIAGCSKGLLVSRFSGNVDPVSGDFSGLVKGG
                     HYIEAGKIVCPVKEVMISGNIYDLLRQVVDVSREYVDVGGSRMPYIQIEGCSITGK"
     gene            complement(2996..4372)
                     /locus_tag="PLM83_01985"
     CDS             complement(2996..4372)
                     /locus_tag="PLM83_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOK70210.1"
                     /translation="MLDLLKSVISGCNDYAEIRYHKRIQDIIEARNGALTKVEHRTLA
                     GAGIRVLADGGWGFVSTNDVTESGLKKALSEAQAAARAGAAVRKDKAQLAAVEPAQGE
                     LVYYDATEEPDIAEKIRALLEAEKRLRERSPLIVGSITRYTQYEDEKYIVTSDGSAVH
                     VLDNKPSFHVFATAGRDGQLETAIKSAGVTGSWADLMRKGDLNTLVDEAVDLAVKKLD
                     AEYAKGGQYIVILDPGLVGVLSHEAIGHTVEADFVMSGSVANGKIGKEVASELVTMVD
                     DGTVAGAAGMVLVDDEGVAGKRTVIIDHGILRDYLHSRESAAAFGMEPKGNARAFTYR
                     DEPIIRMTNTFILPGEDNLDEMIAGIDDGFFLKELGQGGQADATAEFMFGVAEAYRIV
                     DGKVGEMVKGITISGQAFDVLESVDAVSSDFEMAMGAGHCGKFQAAKVDAGGPYLRCR
                     VTVGGRHE"
     gene            5081..5557
                     /locus_tag="PLM83_01990"
     CDS             5081..5557
                     /locus_tag="PLM83_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOK70211.1"
                     /translation="MADKAEVLNDVLVKLFNKILSIEEEAITKAAALGVTMSEIHVIE
                     AIGTDEPKSMSEVASTLGVTMGTLTASVNRLVHKGHVSRFRPEEDRRVVLVTLTEDGR
                     LAHSIHERFHRRMINGILQDLDEESQDAIIVAAEKLYEFFERVKPEDLLLDNDSRQ"
     gene            5738..6727
                     /locus_tag="PLM83_01995"
     CDS             5738..6727
                     /locus_tag="PLM83_01995"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOK70212.1"
                     /translation="MYTKILGTGSALPKQRVTNEDLAMLVDTSNEWITSRTGISERWI
                     STGESASYLAAEAAAKALADADVEPSQIDLVISATVSPDNFTPTVSCEAQKALGLSRA
                     TCFDLWAGCTGFIFSLMTADALVKSGLFSHALVIGVEVLSKLVDWQDRSTCVLFGDGA
                     GAVVLSSASQPGLVTHVSGSDGTRGDVLKIAGIPLANPWMAQHVGESETGSQSVICMN
                     GQEVFKFAVKTMESSIRRVVELSGSSLHDISYIIPHQANNRIIESVAGRMKIPREKFF
                     TNLDHRGNTSSASIPMALDEMNRAGLLLPGMHLVLVGFGGGLTWGASMVRWTR"
     gene            6811..7050
                     /gene="acpP"
                     /locus_tag="PLM83_02000"
     CDS             6811..7050
                     /gene="acpP"
                     /locus_tag="PLM83_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014658851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOK70213.1"
                     /translation="MTLDELKAIISDQLEVSPEEISTGTRFVQDLGADSLDMVELIMS
                     IEEKLGVRIEQQEVKDMVTVGDVVSYIEAHKQAVK"
     gene            7087..8061
                     /locus_tag="PLM83_02005"
     CDS             7087..8061
                     /locus_tag="PLM83_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOK70214.1"
                     /translation="MPVRRLGEKLTRLFGIEYPVLQGGMAWVADARLAAAVSNAGGLG
                     IIASAHLSPDELREEIELARSFAGSHPLGVNIMLASPNAMDVARVCREKKVEVVTTGA
                     GHPAELIGEMKDAGILVIPLVASTAQAKRAVAAGADAVIAEGYEAGGHIGELTTMALT
                     PQVVDSVDVPVVAAGGIADGRGMAAAFCLGASGVQVGTAFLAARECTVHSNYKRKVLQ
                     AKDSDTQVTGRSLGHPVRVLKNRFSREFARMEREGADPDGITAYGMGRLAAAVRDGDV
                     EWGSVMAGQIAGLVTAERTAEEIVRGLVEDAARVLESLRVCQKGECCP"
     gene            8058..9131
                     /gene="fabD"
                     /locus_tag="PLM83_02010"
     CDS             8058..9131
                     /gene="fabD"
                     /locus_tag="PLM83_02010"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOK70215.1"
                     /translation="MKIAYVFPGQGAQYPGMAKEIYDQFASARQVFKTASRTLGYSME
                     RLCFEEGDRLNLTEYTQPALVTASCAVLAAMHESLNLSADTAAGLSLGEYSALVCAGA
                     VDFADAVMLVSKRGRFMQEACPAGAGTMATVLGLDRDCVIDICLDVQKAGAGIVEPAN
                     FNCPGQIVISGETAAVERAAELAQSRGARRVIRLNVSGPFHSSLLSAAGMRLRSELAQ
                     VDITSPTIPVYSNVAARPSMDPDEIRELLVRQVASPVLWEQTVANMAEAGIDVFIEVG
                     PGRTLTGLVKKTVPHAVAMNVEDLSSLEALREFLAEHASVHLPSQVAQVAAAALKTEP
                     ASLRKVSYATQPELVSSSKRAYR"
     gene            9128..9895
                     /gene="fabG"
                     /locus_tag="PLM83_02015"
     CDS             9128..9895
                     /gene="fabG"
                     /locus_tag="PLM83_02015"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOK70216.1"
                     /translation="MTTPRLDNRAALVTGGARGIGLAIAQRLAADGARVAINYCSSDP
                     QVVEKAVMAIESAAAGAGQKVRAYRADVSKHEEVGHMFEQAMSELGRLDIVVANSGIT
                     RDDLAVRMESQAFMDVIETNLLGAFLCAKEAARAMLRQRSGRIVLVSSIVGLDGNAGQ
                     ANYAASKAGLVGLAKSLARELGSRGITVNVVAPGFIDTDMTRALPEKMRDSCIARAAL
                     RRIGSPQEVADAVAFLASEEASYITGQVLRVDGGLVL"
     gene            9946..11184
                     /gene="fabF"
                     /locus_tag="PLM83_02020"
     CDS             9946..11184
                     /gene="fabF"
                     /locus_tag="PLM83_02020"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOK70217.1"
                     /translation="MRRRVVVTGMGAVTPVGCGWRPFWEAISSGQSGIRRIERFDPST
                     CPAKIAGEVREFDPDRYLDRKDRKRMDRFAQYAAVAASQAMEDAGFAAGGAPAPERLG
                     VAFSSGVGGMETFEEQHKRLIERGPEWVSPFFVPMMIANMAAGNIAIMFNAKGPSECQ
                     TTACAASSHSIADAYRLIAEGRADAMIAGGAEAAITPLAMAGFANMQALSRSEDPQAA
                     PCPFDRRRDGFVMGEGSGALILEELDSALARGARIYAEIIGCGFSTDAHHITSPPPGG
                     EGAARAMQEALHDASLAPGDIDYINAHGTGTPYNDLYETQAIKAVFGDDAYRLAVSST
                     KSMTGHLLGAAGAVEAIACVGAITEGFVPPTINYLEPDPECDLDYVANEGRKMEVRRA
                     MSNSFGFGGHNCCIVFTRWE"
     gene            11219..>11436
                     /locus_tag="PLM83_02025"
     CDS             11219..>11436
                     /locus_tag="PLM83_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008231618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HOK70218.1"
                     /translation="MGETGQLRSLDDALALLPHKDPFVFVDEIVYLEPGVKAVGIKRV
                     RPDEFWVPGHFPDRPTMPGVLIIEAMAQV"
BASE COUNT         2484 a         2955 c         3311 g         2686 t
ORIGIN      
        1 atcaatagaa aaccaagcat ctgttccctt ggggggtatc cgcagggaac tacgttgccc
       61 tgaggcaata caggttttgt taaattgcct tggtgttcaa gattaccgtt tatgccgccc
      121 tgcggccagt tgccatttca tttgggatta tgcacctatg tatagctata agttgcatgt
      181 aattgcgttg acatcccgtt gattcccagt gtatcccggg tctgtaaata ctattgtgca
      241 aatggtgttt tcctttcttg acggggagat cacaagtcag ttctcgtcat gtctatgcgg
      301 tcaccaaaga tcaaagcaaa ttgggacatg caaaaggccc agtcgttcac tggcactttc
      361 catttcttta gaatatccat cgtagccaga tataggactt tcctcagcga gttctcgtct
      421 ggaaacacgg acttggtctt ggtgactttc cttagttgcc tgtgatagga ctccacagcg
      481 ttagtggtgt agatcaacct cctgacatcc atggggtagc ggaagtaaga cgtaagctct
      541 acccagttct tctcccactg ccttatcgga attgggtacc cctcgcccca tttttgcttg
      601 agtgagtcaa atgcaagctc agcttgtttt atggtaagag cctggtacac ttgctttagg
      661 tcaatcatga aagccttccg attcttgctt ggcacgtatt tgagtgagtt gcggatttga
      721 tgaattacgc acagctgcat atctgttttt gggaacaccg ctttcactac ctcggagagg
      781 ccgcttagtc catctctgta gactattaag atgtcatcaa cgcttctgga tttgagatcg
      841 tcgcatactt ccagccagaa gcttgcgctt tcctcatcac caatccaagt ccctaagata
      901 tctttgtacc ccgaggagtt tatccctaat gctgtatatg cggccttatt gacgattcta
      961 ctgtcttggc ggacattgaa gtagatggcg tccagaaaaa caatgaggta aacccgatcc
     1021 aatggtcgtg actgccactc ctttatcatc ggcacgattc tgtccgtggt gctgctgaca
     1081 agcccagacg agacgttgat gccatacagc tcccttatct gatctgctat gtcccttgta
     1141 gacatatctt tggcatacat agtgattatc tggttttcaa gcctgtcaat ggccatttga
     1201 gaatcccctt ttttggccat gaaaaatccc cacgggaaga gcaacgggtc tacgagcaag
     1261 agcctcatgt cagcgactgc ctcatatatg cattctgttt ggcaatctaa aagttcatag
     1321 ttcggcaatg aaaaaagtgc ataagttgat caccgtgctc gagggcggta cggggccagt
     1381 gggctttggt aactgtgttc tgaatatatg agccgccgcg tcttaaggag tggtgcagat
     1441 agagtctgag gtacgcaggt tgaactgcag ccgtgaaata ctcaccgggc ttttgaggaa
     1501 agcccggtga tatgggaacc gagaattacc gcgccggaga aatatcaaat caggaaaggg
     1561 cagggggcga aattgcctcc tcttattcta ttttgcctgc tattggcgcc cggcgggctt
     1621 tgctatccgc agctacttgc ctgttataga gcacccctca atctggatgt aaggcattct
     1681 tgagccgcca acatcgacgt attcacgcga tacgtccacg acctgtctga ggaggtcgta
     1741 gatgttgcct gagatcatga cctctttgac cggacacacg attttgcccg cttctatgta
     1801 atgccctccc ttcaccaggc ccgagaagtc accgctcaca gggtcaacat tccctgagaa
     1861 cctgctcacc agcagtccct tggaacagcc cgctatcatc tcgtccagtg agtgtttgcc
     1921 ggatgcaacg acgaagttcg tcgtggaaat gcccggaaca gtatggtcgc tgcctgctgc
     1981 gcgcccattg gaggcgacgc catctttttt agcagtatag cagttataga ggtaatggtt
     2041 gagcgtgcct tcgcttacga gctgcaaagg agcgggcggc accccttcgc ggtcaaatga
     2101 ggtggaccct gccgctttgg gaagcttggg atcatcagtc actgaaatca ccggcgacat
     2161 tacctcggtg cccaacatgc ccgcccattt ggatctcccg ttctgcacat tctgggcatt
     2221 gcatgagaac gatatgggtt cggctattag atcgtagcct gcgtagggag tgatgatcac
     2281 gtcccctttg aaactcggca cggtagtggc cccgagcgaa gcgacgactt tctcggccag
     2341 tttgcgtcct atggctgtag gatcgatttc gtccttcctg cacgtgaccg caacttccaa
     2401 atcgaaagat gacacttctt tttcgtccct ggcaaacccc atagctaggc aggtgaagac
     2461 agaatctttt tcaacgagcc tgatgccctt ggagttgaca atggctttct cagtgatgtc
     2521 caccatatac tgcgctgaat ccaaagtaac tctgggatcg aaggcacgag ctgagttcac
     2581 aagcgctaac ccctgctcta ctgcgtcttc aaggctcatg gtttcagcag attcgtcgta
     2641 tcggccggga accggctcga tcgtggacgg ctcaggcatc cagtgattgg gatcatcagg
     2701 cgacgcattc gctatcgcta tcgcggccag gagcgtctcc tcaagcgagt cgggggagag
     2761 catgttcgtg gcggcaaagc cttgttttgc tccgcccttg ccgtaaagga gtacgcgtat
     2821 gcccaggccg tcatagtcgt cgcccttggg tacctgcagg tcgttttttt cgaatactgc
     2881 cccaagttgt ctggacttga tgtagaacac ttctgcatcg tcggcaccca tcttgcgcgc
     2941 acgttctacc aggctgtcac agagctcgag ttccttcaat gtttcgctcc ctcctctact
     3001 catggcgtcc tccgacggtt acacgacaac gcagataagg ccctccagcg tccaccttgg
     3061 cagcttggaa tttgccgcag tgtcctgcgc ccatcgccat ctcaaaatcg gaggatactg
     3121 catcgacgct ctcaagaaca tcaaatgcct ggccggagat ggtgatgccc ttgaccatct
     3181 ctccaacctt cccgtccact attcggtagg cctcggcgac gccgaacatg aactctgccg
     3241 tggcgtcggc ctggccgccc tgaccaagtt ccttaagaaa gaacccgtcg tctatcccgg
     3301 caatcatctc gtctaagttg tcctcgccgg gcagtatgaa agtgttagtc attctgatta
     3361 tgggctcatc gcgataagtg aaggcccgcg cattgccctt tggctccatt ccgaaggctg
     3421 ctgctgattc gcgggaatgc aggtaatctc tgaggatgcc atggtcgatg atgaccgtgc
     3481 gctttccggc cacaccctca tcatccacca aaaccattcc ggcggcgccg gctacagtcc
     3541 cgtcgtccac catggtcacc agctccgagg ctacttcctt cccgatcttg ccattggcaa
     3601 ccgaaccgct catcacgaaa tcggcttcta cggtgtggcc tatggcctca tgcgatagga
     3661 ctccaacgag ccccgggtcg agtatgacta tgtactgtcc gcctttggca tactccgcat
     3721 cgagcttctt tacggccagg tccacagctt catccacaag tgtgttgagg tcgcccttgc
     3781 gcatcaaatc agcccaggag cctgtaacgc cagcgctctt gatcgccgtt tcaagctggc
     3841 catcgcgccc ggcagtggcg aacacatgga agctcggctt gttatcgagc acatggacgg
     3901 cggacccgtc tgaggtgact atgtacttct cgtcttcata ctgtgtatac cttgtaatgc
     3961 tgcctacaat gagaggagat cgctcgcgaa ggcgcttttc ggcctcaagg agtgcgcgaa
     4021 tcttctctgc gatgtcgggc tcctcggttg catcgtagta gacgagctct ccttgggcag
     4081 gttcaacagc agccagttga gctttgtctt tccggactgc cgctccggcc cgggctgcgg
     4141 cttgggcttc agatagagcc tttttcagcc ccgactcggt cacgtcgtta gtggagacaa
     4201 atccccatcc tccgtccgct aaaactctga tacccgcccc cgccagggtc ctatgctcca
     4261 cttttgtgag cgcgccattt cgggcttcga tgatatcctg aatgcgcttg tgatatctga
     4321 tttctgcgta gtcgttgcag cctgagatga ctgactttaa caaatctagc attacatcac
     4381 cctccttgaa taagtactgt cttcggttcg gggcgcaata gctgggtgaa cagggtcgaa
     4441 ccgaagatgc tgggaagtgc cgagtgcttt cagagatgcc cgaaaaccat gtcacatcgc
     4501 cacaccacga cgttgccgca aaatggtcag tgcagggagg cgcgttcact ggggaggctg
     4561 cgcctgcgcc ggtctagccg gcgatacatc acctgcccgg taaagagcgg gagttctgcg
     4621 ggtttgacaa tgcgtcctct aactcagtta aggtatgtat catcgtgatt tccggtaatg
     4681 tcaggttttc atctttccct ttcagccaga tgctgtccgt gcccgcctct atgcaaggga
     4741 gtacatctat tttgatatta tctcctacat agacgcaatc tcaaatatca acattaactc
     4801 ttctccggcc tctgtccttc gagttttcgg aaagaagact cacttcataa taacgtctgt
     4861 agagtctcaa ttgtttgccg gctgagatgg ttttgacaac atgccggaac tgcttgagga
     4921 aagcgagcgc gcgctcttga gcctttttgc ccccgacgaa gcatcctaca gcgtcgcgcc
     4981 tgcccctcct gagaagaatc cctgatgctc ttcctgcaat ggccggatgt acggtaagat
     5041 ggtgttgaaa gatgcacttg gaatgaggag gggcgggtga atggctgaca aggctgaagt
     5101 tttaaacgat gtactggtaa agctgttcaa caagatattg tcgatcgagg aagaggcgat
     5161 aacgaaggca gccgctttag gggttacgat gtctgaaatc catgtcatcg aggcaatagg
     5221 tacggatgag cccaagtcga tgtcggaagt cgcctcaacc ttaggcgtaa caatgggcac
     5281 gctaactgct tctgtcaaca gattggttca caaaggccat gtttcgcggt tccgtccgga
     5341 ggaagatcgg cgagttgttc ttgtgaccct caccgaggat ggtcgtcttg ctcattcaat
     5401 ccatgagagg tttcataggc ggatgatcaa tgggatattg caggatcttg atgaggagag
     5461 ccaagatgct atcatagtgg ctgctgagaa gctatacgag tttttcgaac gggtgaagcc
     5521 tgaagacttg ctgctggata acgattcccg gcagtgaccc caatgttctg tccccaagac
     5581 attttgcccc catcaattgc acttgacaac gtcttgcttt ggccttaaac tttgaataac
     5641 aaacaatttg aagcacaaag taaagaacgc ggcatgctgc tttctggtct tgggcgaacc
     5701 tccaaggcag gagcggcagg cctcgggggc atgcaagatg tacacaaaga tcctgggaac
     5761 cggaagcgca cttccgaaac aacgcgtaac caatgaggat ctggctatgc tcgtcgatac
     5821 gtcgaacgag tggataacgt cacgcaccgg catatcggag cgatggatat ccactggaga
     5881 gagcgcgtca tacctggctg cagaagcggc agccaaggct cttgcagacg ctgatgtgga
     5941 gccttctcaa atagacctgg tgatttcggc cacagtatca cctgacaact tcactccgac
     6001 tgtgtcgtgc gaggcgcaga aggccttggg cctttcgcgg gcgacatgct ttgacttgtg
     6061 ggcgggatgc accgggttta tcttttctct aatgacagcc gatgcgctgg tgaaatcggg
     6121 cctcttctcg catgcgctcg tgatcggcgt tgaagttctt tcaaaactgg tcgactggca
     6181 agatcgtagc acatgcgtcc tcttcggaga cggggcggga gcagtggtgt taagctctgc
     6241 cagccagcca ggccttgtga ctcatgtatc cggctctgat ggaacccgag gcgacgttct
     6301 caagatagcc ggtatcccac tagctaatcc gtggatggct caacatgttg gggagtcgga
     6361 gacaggctcc cagagcgtga tctgcatgaa tggccaggaa gtcttcaagt ttgcagtcaa
     6421 aaccatggaa tcgtctatac gccgcgtagt ggagctgtca ggctctagcc ttcacgacat
     6481 ttcctacatc attccccacc aggccaacaa cagaatcata gaatcggttg cgggcaggat
     6541 gaaaatcccc cgcgaaaaat tcttcacaaa cctcgatcac agagggaata cgtcctccgc
     6601 cagcattccg atggccctgg atgagatgaa tcgagcgggc ctgctcttgc ccggaatgca
     6661 tctggtgctt gtggggtttg gcggaggtct gacgtggggc gcaagtatgg ttcgatggac
     6721 gcggtaatga taaggctccg ttgaaggggc gacaatacct tgacctaacg agaaagaaga
     6781 gagagccgca aggctgagga gggttactat atgacattgg atgaattgaa ggcgatcatc
     6841 tccgatcagc tggaggtatc cccggaagaa atatcgactg gaaccagatt cgttcaggat
     6901 ctaggagccg actccctcga tatggtggaa ctgatcatgt cgatcgagga gaagcttggg
     6961 gtccgcatag agcagcagga agtgaaggac atggttaccg tgggagatgt ggtgtcgtac
     7021 atcgaggcgc acaagcaggc tgtgaagtag gcgggccgga tatggtatga ggcgggtgat
     7081 gtaaggatgc ctgtgaggcg cctgggagag aagctgacgc gtctttttgg catagagtat
     7141 ccggtgctgc agggcggaat ggcttgggtg gccgatgcac ggctggcggc tgcggtgtcg
     7201 aacgccggag gacttgggat tattgcatcg gctcacttgt cccccgatga gcttcgggaa
     7261 gagatcgaat tggctagaag cttcgccggc agtcatcctc tgggagtcaa cataatgctg
     7321 gcaagcccga atgctatgga tgttgcgagg gtgtgccgtg agaaaaaagt tgaggtcgtg
     7381 acgaccggtg ctggccatcc agccgagttg ataggggaaa tgaaggatgc gggcatcctg
     7441 gtcatcccgt tggtggcttc aacggctcag gctaaaagag cagtggctgc gggcgctgat
     7501 gccgtgatag cagagggcta cgaggccgga gggcatatag gcgagttgac cactatggct
     7561 ttgacgcctc aggtagtcga ctcggtcgat gtgccggtgg tggctgcagg aggtatcgcc
     7621 gatggccgtg gcatggctgc cgccttttgc ctgggcgctt caggggtcca ggtaggcacc
     7681 gcatttcttg cggctcgcga atgcactgtc catagcaact acaagcgcaa ggtgctgcag
     7741 gccaaggact cagacactca ggttacaggg cgcagcctgg ggcatcctgt gcgcgtgctg
     7801 aagaatcgat tctcgcgcga atttgcccgc atggagcgcg aaggcgcaga tcccgacgga
     7861 ataacggctt atggcatggg cagacttgct gccgctgtcc gtgatggaga tgtggaatgg
     7921 ggctcggtta tggccggcca gatcgcgggg cttgtaactg cggaacggac ggctgaggag
     7981 atcgtgcgcg gtctcgtgga agatgccgcg cgtgtcctgg aatcgttgcg agtatgccaa
     8041 aaaggggagt gttgcccttg aagatagctt acgtctttcc cggacaggga gcacagtatc
     8101 cggggatggc caaagaaatc tacgatcagt tcgcgtcggc ccgtcaagtc ttcaagactg
     8161 ccagcagaac tctgggctac agcatggagc gcctgtgctt tgaggaaggc gacagactga
     8221 acctgactga gtatactcag cctgcactgg tcactgcgag ctgtgcggtt ctggcggcta
     8281 tgcacgagtc cttgaatctt tcggcggata ctgcagcagg gctaagcctg ggtgagtatt
     8341 ctgcgctcgt ttgtgcaggg gcagttgatt ttgccgacgc tgtgatgctt gtcagcaagc
     8401 gcggacggtt catgcaggaa gcctgtccgg ccggcgccgg tacaatggcc acagttctcg
     8461 gactcgaccg cgactgtgtg atagacatat gccttgacgt acagaaggca ggcgcaggca
     8521 tcgttgaacc tgccaatttc aattgccctg ggcagatcgt catctccggc gagacggcag
     8581 cggtggaacg cgcggctgag cttgctcagt cgagaggcgc tcgccgggtg ataagactga
     8641 atgtatcagg gcctttccat tcgagcctgc tttctgcggc gggaatgcgc ctccgcagcg
     8701 aacttgcgca ggtggatatc acaagcccta caatcccagt ttactccaat gtagccgcga
     8761 gaccttcaat ggacccggat gaaatccggg agttgcttgt gaggcaggtg gccagtcccg
     8821 tactgtggga gcagactgtt gccaacatgg ctgaagctgg aatagacgtt ttcatcgagg
     8881 ttggcccggg cagaacgctt acaggccttg tgaagaagac tgtgccccat gcggtggcga
     8941 tgaacgtgga agacttgtct tcccttgagg cgctcagaga gtttttggcc gagcatgcat
     9001 ccgtgcatct tccctctcag gtagcgcagg ttgcggctgc agcacttaaa acggagcccg
     9061 cgtccttgcg aaaggtttcg tacgcaaccc aacccgagct cgtgtcttct tcgaagaggg
     9121 catatcgatg actacgccga gactggacaa cagagcagcc cttgtaacag gaggggcccg
     9181 ggggataggc cttgccattg cccaacggct tgccgctgat ggcgctcgcg ttgccatcaa
     9241 ttattgttcc tcagaccctc aagtggttga aaaggccgtc atggctattg aaagcgcagc
     9301 ggccggggcc gggcagaaag tgcgggccta tagggcagat gtgtccaaac atgaagaggt
     9361 tgggcacatg ttcgagcaag caatgtccga gttgggccgt ctcgatatcg tggtagccaa
     9421 ttcggggata acccgcgacg acttggctgt gcggatggaa tctcaggcct tcatggacgt
     9481 gatcgagacg aatcttctcg gcgcttttct ctgtgccaaa gaggcggccc gggccatgtt
     9541 gcggcagcga tcaggcagga tcgtgctggt ctcgtcaatt gtcggactgg atggaaatgc
     9601 gggacaggca aactatgctg cgagtaaggc cgggcttgtg gggctggcaa agtcgctcgc
     9661 tcgcgaactc gggtcccgcg ggatcaccgt gaacgtagtc gcccctgggt tcatcgacac
     9721 cgacatgacg agggcccttc cagagaagat gcgcgattcg tgcatcgccc gggctgctct
     9781 ccgcaggatc gggtcgcctc aggaagtggc cgatgccgta gcgttcctcg cttctgaaga
     9841 ggccagctac atcacgggcc aggttcttcg agtagacgga gggctcgttt tgtaacttga
     9901 tatttctcgg gtgcccagta tgcgggcgta attgaggtgt ttgttatgag aagacgagtt
     9961 gtggttaccg gaatgggggc agtgacgccc gtgggatgcg gatggcgtcc tttctgggag
    10021 gccatcagtt ccggacaatc aggcattcgt cggattgagc ggttcgatcc gagcacgtgc
    10081 cctgctaaga ttgcagggga ggtgcgcgag tttgatccag accggtatct tgaccgcaag
    10141 gatagaaagc gaatggacag gtttgcgcaa tatgctgcag ttgctgccag tcaggccatg
    10201 gaggatgcgg gttttgcggc agggggagcg ccggcccctg agcggctggg tgtggccttc
    10261 agttcgggcg tgggaggtat ggagacattc gaagagcagc ataaaaggct catcgaacgt
    10321 ggaccagagt gggttagccc attcttcgtg cctatgatga tcgccaacat ggcggccggc
    10381 aatattgcca tcatgtttaa cgcaaaaggt ccaagcgaat gtcagacaac tgcctgcgcg
    10441 gcgtcgtcgc acagcattgc cgacgcctac aggctcatag cagagggacg ggctgatgcc
    10501 atgatagcag ggggcgccga agctgcgatc actccgcttg ccatggctgg atttgccaac
    10561 atgcaggccc tatcccgaag tgaggatcct caagctgcgc catgtccttt cgatcggcgg
    10621 cgcgacggat tcgtgatggg cgaaggatct ggcgcattaa tactggagga gcttgactct
    10681 gcattggccc gaggggctag gatatatgct gagatcatag gttgcgggtt ttccacagat
    10741 gcgcaccata ttaccagtcc tcctcctgga ggggagggag cggctcgcgc catgcaagag
    10801 gcgcttcatg acgcgtcatt ggcgcccggc gatatcgact acatcaacgc ccacggcaca
    10861 gggacgccgt acaatgatct ttatgaaacc caagctatca aggcagtctt cggagacgat
    10921 gcctatcggc tcgcggtgag ctcgacgaag tccatgaccg ggcacctgtt gggcgcggca
    10981 ggcgcagtgg aggctattgc ctgtgtaggc gcgatcacag aaggctttgt gccgccgacg
    11041 atcaactacc tggagcccga ccccgaatgc gacctcgatt acgtagcaaa cgaaggacga
    11101 aagatggaag ttcgcagagc catgagcaat tcctttggct tcggcgggca caactgctgc
    11161 atagttttta cccgatggga ataggcagaa tcggatgaat cggacggagg cgaaggaaat
    11221 gggtgagacg ggccagttgc gttcgctaga tgatgcgctt gcattactcc cccataaaga
    11281 cccatttgta tttgttgacg agatcgtcta tttggagccc ggagtcaagg ctgtagggat
    11341 caaaagggtc aggccggacg aattttgggt gcctgggcat tttccagatc ggccgaccat
    11401 gccaggggtg ttgataatag aggccatggc gcaggt
//
LOCUS       DAOSAI010000028        11174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3882_length_11174_cov_4.239950, whole genome shotgun sequence.
ACCESSION   DAOSAI010000028 DAOSAI010000000
VERSION     DAOSAI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11174
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3882_length_11174_cov_4.239950"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(617..1159)
                     /locus_tag="PLM83_02030"
     CDS             complement(617..1159)
                     /locus_tag="PLM83_02030"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOK70219.1"
                     /translation="MSFRFPRQDVRAKQNNVEPPHEAESYESRAGASGPASCVAGCSF
                     GVGRGVEGAPKKEKEAAESAEEVELLVTFPSVHHALKLERMIKTCACGQDGSGRSVDL
                     MPVPRRISSSCGLAASVCVTMGAGSTSGNGVARLGSPGTVCRSLVEELKAAGVEYQGI
                     YLPLPGGEFQLLTDGEEAGM"
     gene            complement(1122..1352)
                     /locus_tag="PLM83_02035"
     CDS             complement(1122..1352)
                     /locus_tag="PLM83_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009279219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOK70220.1"
                     /translation="MATQVDARGLSCPQPVVLTKKAMDSITSGEIEVLVDNPAARENV
                     ARFARNAGCSVEIGEERDGSYVISISKAGRKS"
     gene            complement(1366..2466)
                     /gene="yedE"
                     /locus_tag="PLM83_02040"
     CDS             complement(1366..2466)
                     /gene="yedE"
                     /locus_tag="PLM83_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HOK70221.1"
                     /translation="MNQRRVVIVAGAIVGALSIILVATGNPGNMGICVACFIRDIAGA
                     LGLHRAPIVQYIRPEIPGFIIGSMIAAMAAGEFKTRGGSSPLLRFVLGFFVMIASLVF
                     LGCPLRMVLRLAGGDLNALLGIAGFAFGIWIGTLFLKRGFTLGRSTAMHKGNGAVMPL
                     ISIALIALLVAAPAFIFFSETGPGSMHAPLILALLAGIIVGVVAQRSRLCLAGGIRDL
                     ILIRDPHLFNGFVAIFAVAALLNLLTGRFQFGFDGQPVAHTDGLWNFLSMSVVGLGAV
                     LLGGCPLRQCILSGEGDGDAAIAFLGMLVGAAFAHNFGLASSPAGVTGAGKAATLIGL
                     AVMLAIGYFGAVNAVDALSTVGGGKKTAGGSR"
     gene            2764..3744
                     /locus_tag="PLM83_02045"
     CDS             2764..3744
                     /locus_tag="PLM83_02045"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HOK70222.1"
                     /translation="MDIKQLSIFLAVANHGSFTEAAVSLFMSQPTISVQIAALERELG
                     VTLFERKSHGVTLTPAGQVLKPYAADILAMRDRAAEAIDHYKSDFSGTLTLLASSVPA
                     GYVIPEFVAEFLAEYPKVFIQLRHADSQEVWDRILNYDADLGIVGSIGNEASIESFPI
                     IEDEVILIAPPKSKYAAWGDIIDIESLLAEPIVCREPGSGTQVTFDKALQEHGIDPRG
                     LNIRARLESAEAIKAAVAADAGLGLGIVSARAAAAEIAERKLKAVRIKGIDLHRQLYM
                     INHKRKVLSPTALAFRDFVLERLLHPRESTADELEMTRKRHMMQKERDYS"
     gene            3780..4958
                     /locus_tag="PLM83_02050"
     CDS             3780..4958
                     /locus_tag="PLM83_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOK70223.1"
                     /translation="MAKIYLDNAATSFPKPPQVWEAMQQYMLNIGASPGRGGYSLSLD
                     AGRLTLNARIAAARLFRAPSEEHVVFTPNVTYALNCAIHGLLRPEDHVVTTSMEHNSV
                     IRPLCHAKDQNRVEVSIVGCDSLGRLDPDDIRKAIRPNTKMIVMTGASNVVGTILPVA
                     DVGRIAAEAGAFFVVDAAQTAGVIDLDFGALGADILAFTGHKALYGPPGTGGMIVSDR
                     AAAEMLPLAQGGTGSKSDEERQPGFLPDKFEPGTPNTVGIAGLAAGMQFVQKTGPDRI
                     RRHEAELASRFVQQAVDIGPKVRVLGPWGSGEASADDAVATVSISFEDGCIDPGRLAY
                     ELDAEFGVMVRSGLHCAPLAHKTVGTFPEGTVRFSFGWFNTIADVDRAATALAELASE
                     "
     gene            complement(5076..5216)
                     /locus_tag="PLM83_02055"
     CDS             complement(5076..5216)
                     /locus_tag="PLM83_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70224.1"
                     /translation="MYERKVGFGLFYPFKLMMDAGGTDVIHLQDVSIREMELTEKPCK
                     GN"
     gene            5311..6459
                     /locus_tag="PLM83_02060"
     CDS             5311..6459
                     /locus_tag="PLM83_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK70225.1"
                     /translation="MTIKPLSKSLRLKNPAHARHFRQSPLAITMAILALLALSGSGGL
                     CAVAQPEPLELTLDEARSYAIDHSYDYAMAVLEFEASEIQFQMAEADALIRPSVIASK
                     RAAGARRDARRALVAKEQSLMLDVDEAYYNLVTAQERANIIHASEEQARESLRIVRLR
                     FEAGLASKMDVMSAEIALEQAVTSTLSAKNHLELAQLALKRTLGMDLDTPVIAIDSAQ
                     PIAGDVDYEAALALALKNRPEIVKARETLEICELEAKFADNEYTPELTKRQLNNALAQ
                     ARLAAGQAERGVRVEARQMYLSLEESHRAISIASAATKQAEENYRIMKLRYEHGMEIA
                     NSLLGAQLSLTEARLSELQSIMSYNIARLHFDAWANYPAEEAEPGDPR"
     gene            6456..7970
                     /locus_tag="PLM83_02065"
     CDS             6456..7970
                     /locus_tag="PLM83_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK70226.1"
                     /translation="MSQTAQHKSQQRQTAFVTVPFSRRAADICICVVMLLLALAPCTF
                     AADSSLGASHADESLALTMEDVMRLAESSSPSVASAKRAVELAMLQLQAAEAQKRPSI
                     TVHAAPLQIGIGPLSVQSVVEEDSKPMGKGETSMYALSQAGGDVRIPLGGGSLSLSAT
                     ASFAFSDPALSGKSAWNGSASLSLPILGASSPGEAPSSTSSAQSQLRSAQFQLDQSIR
                     QARASAQAAYFNLLAAEDRVAAADRGLSRARTHAAFVEGRCSAGNASELDALEAKAGA
                     DRAEAAAQSARHNLAIASISFNRAIGRDLETLVHLAPAGEYVRWPEDLDLDTCTRAAL
                     ETRPEIPMAEQDVADAEAALAQAIAAKRPDVFFQGQASSGGHWRVGLEVSALLTRDYA
                     AEIAIESAKGRVEQAKANAVETRDSILLEVVEAFYNLREAESSVELARLAVAIATATL
                     DIREEQCRIGAVAYAEVSEAIDAVRKAESDLAYQLAACFMAKSRLLQSVGSDNI"
     gene            complement(8011..8724)
                     /gene="hisIE"
                     /locus_tag="PLM83_02070"
     CDS             complement(8011..8724)
                     /gene="hisIE"
                     /locus_tag="PLM83_02070"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOK70227.1"
                     /translation="MIERLESDDGESLEGWIDTLKFGADGLIPAVIQESVTGQVLMVG
                     YMNRQSIEMTLREGRTCFWSRSRQRLWTKGETSGHFQKVRAVFCDCDRDCLLIEVDQT
                     GEACHLGRKSCFFTQLWGSADAAGTGFSLDKLYETVEDRKASQPEGSYTAKLIRSGQD
                     RVLKKVAEEAGEVMLASKNDDREEIIREMADLWFHSIVVLAYHDIPPQAILDELADRH
                     SNARKAESLCTERKEGPGY"
     gene            complement(8721..9587)
                     /gene="hisF"
                     /locus_tag="PLM83_02075"
     CDS             complement(8721..9587)
                     /gene="hisF"
                     /locus_tag="PLM83_02075"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOK70228.1"
                     /translation="MRAAGSRRIIPCLDTKDGRVVKGVNFENLRDAGDPVGLAEIYES
                     QGADELMLLDLSATAEGRRTALDLVGRVASAVSIPLSAGGGITSLDDVGRVLDAGASK
                     VCINSAAVRNPQLLQHAAKAFGVDRIVSAIDASAIAEPFLDAGNRHGDRDVNDIVSIE
                     VDSKNDGNGDGCGRWVVCTFGGKQRTDLDLIEWARTVERLGAGEILLTSVDRDGSTDG
                     YDLRQLRAVTQAVGIPVIASGGAGTPEHFRDAFVEGGADAALAASVFHFGTLSVGDVK
                     RHLKREGVDVRL"
     gene            complement(9603..10292)
                     /gene="hisA"
                     /locus_tag="PLM83_02080"
                     /pseudo
     CDS             complement(9603..10292)
                     /gene="hisA"
                     /locus_tag="PLM83_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
     gene            complement(10294..10908)
                     /gene="hisB"
                     /locus_tag="PLM83_02085"
     CDS             complement(10294..10908)
                     /gene="hisB"
                     /locus_tag="PLM83_02085"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOK70229.1"
                     /translation="MPKPRLAEVHRKTSETSVELRLILDAARDHGCSGRHQANISTCC
                     GFFDHMLRLLAFHAQFSLDLHATGDIEVDYHHLVEDVGICIGEAFCEALGDKQGIARF
                     GSALVPMDDALSMAVVDLSGRPFLHFNVPMPCAKVGDFDTELVEEFMRALASNGKFTL
                     HVDLHHGWNTHHIIESVFKAVALALRQAAAAGDTSSIPSTKGVL"
     gene            complement(10910..>11174)
                     /locus_tag="PLM83_02090"
     CDS             complement(10910..>11174)
                     /locus_tag="PLM83_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70230.1"
                     /translation="FPSCTNFILFRTTMPDATMVRDALRRYGVSVRVFPDEPMLRGCL
                     RVSCGTKEEDDAFLEALEKVLAEANQTSAGLRDDDGKRIEGGE"
BASE COUNT         2629 a         3390 c         2839 g         2316 t
ORIGIN      
        1 atccgaacac gtcttccaat ctccaggttt cctctaccgg aacctccgaa cgcaatggtc
       61 tcctattcaa cccggagcca cctccaattt caatgcgcag ttccatatat tgttcttgtt
      121 actattcctc agacctcaat gatttccctg cccgtgtttg cttcaaattc cgtggtttgc
      181 tcgccacttt ccgagctcag ctcagcagcg atttgggaaa atcagaacct ccagagtagg
      241 caatcgggga gactgggcgc atccgcgctc ccctaacatt caccagctct cgctcccata
      301 ttcgaaaacg cgtccaaccg cataggcaaa ccaacttgag tgaaacagga gatgaattgg
      361 ttgagcacgc atgcgtacat gcgtcaaatc agactggatt tataggagat gggtgcaagt
      421 tggcatttaa agcaacatca atctattaat gcccgcacca gctggccaga tgaaacgctg
      481 caagcaaggt gaacttcgca gcgcaggcca ggcaaaggac ttcccatatt gggcctggga
      541 cggtttttag gtaaatcaga ataaacggcg aaactatcat ctcatctcgc cgtccgcctg
      601 gtctatcccg ggttcgctac atccccgcct cttcaccatc ggtcagaagc tgaaactcgc
      661 cgccaggcag aggcaggtat atgccctggt attctacgcc agccgccttc aactcctcca
      721 ccaaagaacg gcaaacagta cccgggctgc ccaatcgggc aacaccattc ccggacgtac
      781 tccccgcgcc catggtcacg caaacgctgg cagccagacc acagctggaa cttatccggc
      841 gtggaacagg catcagatcg actgaccgtc ccgagccatc ctgcccacac gcacatgttt
      901 tgatcatccg ctccaatttg agcgcatgat gtacactggg gaatgtaaca agcagctcca
      961 cttcttctgc cgactcagcc gcttcctttt cctttttcgg cgctccctcg actcctcttc
     1021 cgaccccaaa cgaacatccc gcaacgcaac tagcagggcc ggaggcaccg gccctgctct
     1081 cataagattc agcctcatgg ggcggctcaa cgttattctg cttagctctt acgtcctgcc
     1141 ttggaaatcg aaatgacata cgagccatcc ctctcctcac caatctccac cgaacatcca
     1201 gcattcctcg caaatcttgc gacattctcc ctggcagcag ggttgtccac gagcacttcg
     1261 atttctcctg atgtgatcga atccatagcc ttcttagtaa gaactacagg ttgaggacaa
     1321 gaaagccccc tagcgtctac ttgtgttgcc atggtccatc cccccttatc tgctgccgcc
     1381 agcagtcttc ttgccgccac cgactgtact caatgcgtca acagcgttta ctgccccaaa
     1441 ataaccgatt gccagcataa ccgcaagtcc gatcaaggta gcagccttac ctgcgccggt
     1501 cacccccgct ggcgaagacg ccaggccgaa gttgtgcgca aatgccgccc ccacaagcat
     1561 cccgaggaat gcaatcgcag catctccgtc accttcgcca gagagtatgc attgacgaag
     1621 cgggcacccg ccgaggagca ctgcaccgag tcccaccact gacatgctga ggaaattcca
     1681 caggccatca gtatgggcca caggctgtcc gtcaaatccg aactggaacc tgcctgtgag
     1741 gaggttgagg agtgcggcta ctgcgaatat ggccacaaaa ccattgaata ggtgtggatc
     1801 gcgtatcaat atcaagtctc tgattccccc tgccaagcag aggcgcgaac gctgtgctac
     1861 caccccaacg atgattccag caagcagcgc cagtatgagc ggtgcgtgca tcgagccggg
     1921 accggtctca ctgaagaaga tgaacgcagg agcggccact aaaagcgcga tcagcgcaat
     1981 gctgatcagc ggcattacag ccccgtttcc cttatgcatt gctgtgcttc ttcccaaagt
     2041 aaagccgcgc ttgaggaaga gtgttcctat ccatatgccg aatgcaaaac ccgcaatgcc
     2101 caaaagcgca ttgaggtcgc cgcctgccag gcgcaaaacc atcctgagcg ggcatcccaa
     2161 gaataccaat gacgctatca ttacaaaaaa gccgagaaca aaccggagaa gcggagatga
     2221 tccgcccctg gtcttgaact cccctgcagc cattgcagcg atcatcgatc cgatgatgaa
     2281 gcccggaatc tcggggcgaa tatactgaac tatcggagcg cggtgcaatc ccagagctcc
     2341 cgcgatgtca cgtataaagc acgcaacaca tatccccata ttgcccggat tgcccgttgc
     2401 caccagaatg atgctgagcg ctcccacgat agcgccggca actattacga cacgacgttg
     2461 gttcataacg acgacctgac ctcccttaga acatggactc caactataaa atggagaaaa
     2521 tgtagtctcg cacaatgtgg agccaattga agtatatgac catctccagg cgttcacaat
     2581 acagactatt aataattcgt atcaattgaa ttacgctatc taatatcatg ttgacaatgg
     2641 cggtgcgccg cgagatactt gcataagggg gcaagcggcc cgcgatggga cgcgtgtttt
     2701 tccctgccgt caacgattgc cgttggccat gcttaccttg aagccggaga gtgaattgac
     2761 atcatggaca taaagcaact ctctatcttt cttgccgtcg ccaaccacgg ttcctttacc
     2821 gaggccgcag tatccctttt catgtcgcag ccgaccatca gcgtgcagat tgcagcactc
     2881 gagcgcgaac taggggtcac acttttcgaa aggaaaagcc atggagtgac acttactcct
     2941 gcaggtcagg tgctgaagcc ttacgccgcc gatatcctcg ccatgcgcga tcgagcagcg
     3001 gaagccatcg accattacaa gtcggacttc tcgggaaccc taacattgct ggccagcagt
     3061 gttccagcag ggtatgtgat cccagagttc gtggccgaat tcttggccga gtacccgaaa
     3121 gtcttcatcc agttgcgcca tgcggattcg caagaggtgt gggacagaat tctcaattac
     3181 gatgcagatc taggcatcgt cggatcaatt ggcaacgagg ctagcataga atcatttccc
     3241 atcatagaag acgaagtgat attgatcgct cctccaaaaa gcaaatacgc ggcttgggga
     3301 gatataattg acatagaatc gttgcttgcc gaacccatcg tctgccgcga gccggggtct
     3361 ggcacccagg tcacattcga taaggcactg caggagcacg gcatagaccc ccgcggactc
     3421 aatatcagag cccgccttga aagcgcagag gcaatcaagg cggcagttgc agcagacgcg
     3481 ggattggggc ttggcatcgt gtcagcgcgg gccgcggctg cggagatagc ggaaagaaag
     3541 ctgaaagccg tgcgcataaa gggcatcgac ctccatcggc aactttacat gatcaaccac
     3601 aagcgaaaag tcctatcacc tactgctctg gccttccgag atttcgtctt ggagaggctc
     3661 ttgcatccaa gagaatcgac tgcggacgaa ttggaaatga cgcgaaaacg acacatgatg
     3721 cagaaagaaa gggactacag ttgaatatag acgattgtcg ctccatcaag tcttcaacca
     3781 tggcaaagat ttatctggac aacgctgcaa cttcgttccc caagccgcct caggtatggg
     3841 aagcgatgca acagtacatg ctcaacatcg gagcaagccc cgggcgggga ggctactccc
     3901 tcagccttga tgcagggcgt ctgactctga atgcgcgcat tgcggcggca aggctctttc
     3961 gcgccccttc agaggagcac gtcgtcttca cccccaacgt aacatacgcc ctaaactgcg
     4021 ccatccacgg cttgctccgc cctgaagacc acgtggtaac gaccagcatg gagcataact
     4081 cggtaatcag gcctctttgc catgcaaagg atcaaaaccg cgtcgaagta tcgatagtcg
     4141 ggtgcgattc cctaggcaga cttgacccag atgatatcag gaaagcgatc agaccgaaca
     4201 caaagatgat agtgatgact ggagcttcca acgtcgtcgg gacaatcttg ccagttgccg
     4261 atgtaggtcg aatcgcggcc gaagcaggag ctttcttcgt tgtggatgca gcgcaaaccg
     4321 ccggcgttat cgacctcgat ttcggggccc tcggtgccga catcttggct ttcacaggcc
     4381 acaaggcact atacggcccg ccaggaacag gtggaatgat cgtgagcgac agggcagccg
     4441 cggaaatgct cccactcgct cagggaggca ccggaagcaa gtctgatgaa gagagacaac
     4501 ccggatttct accggataag ttcgaaccgg gaactccgaa taccgtcgga atagccgggc
     4561 tggccgcggg catgcaattc gttcaaaaaa ccggtcctga ccgcataagg cgtcacgaag
     4621 ccgaattggc cagtcgattc gttcaacagg cggtcgacat tgggccgaaa gtccgcgttc
     4681 tcggaccgtg gggctcgggt gaggcgtctg ccgatgacgc agttgccacc gtgtcaatca
     4741 gctttgaaga cgggtgcatc gacccgggaa gactcgccta cgagctcgat gcggaatttg
     4801 gagtgatggt aaggtcggga cttcactgcg ctcccctagc gcacaagact gtgggcacct
     4861 tccctgaagg cacggtccgg ttcagtttcg gatggttcaa taccatcgct gacgtcgaca
     4921 gggcagccac ggcattggcg gaacttgcat cagagtgagc gaggcaacag ccacaggcta
     4981 tcatatagtt gctgcgtgct catgcgttgg cttatggcgg ctcccggcgg acagccagcg
     5041 atcaccctat ggggtaagct ggcgctccac cgtagtcaat ttcctttgca aggtttctca
     5101 gtcagttcca tttcccgtat gctcacatcc tgcagatgaa tcacgtctgt gccgcctgca
     5161 tccatcatca atttgaacgg ataaaacagc ccaaagccta ccttcctctc gtacatacac
     5221 ccttcatgtg cctccgaagg aggaaccggc cgtacggcga actacctatt atcagaccca
     5281 agtgattccc gttaggagga ctttggaata atgacaatca aaccgctttc caaaagtttg
     5341 aggttgaaaa atcccgctca cgcccggcat ttccgacagt cacctctagc cataaccatg
     5401 gccattctgg cgcttctcgc cctctccgga tccggcgggc tatgcgcagt agctcagcct
     5461 gagcccttgg aattgaccct ggatgaagcg cgcagctacg cgattgacca cagctatgat
     5521 tatgccatgg cagtgctcga gttcgaagca tccgaaatcc agttccagat ggcagaggca
     5581 gatgccctca taaggccatc cgtgatcgcc tccaaacgcg cagccggcgc cagacgggac
     5641 gctagacgtg cccttgtcgc aaaagaacaa tccctcatgc tcgatgtaga cgaggcctac
     5701 tacaatcttg tgacagcgca agagcgtgcc aacataatac atgcctccga agaacaggcc
     5761 agggaaagcc tgcgaatcgt tcgtcttaga ttcgaagccg gcctggcaag caagatggat
     5821 gtcatgtcag ctgaaatcgc tctggagcag gcagtaacct cgacgttgtc tgccaaaaac
     5881 catcttgaac tggcacaact ggccctaaag cgcaccttag gcatggacct tgacacacct
     5941 gtcattgcga tcgattcagc ccagccaatt gccggagatg ttgactacga agccgcactc
     6001 gcccttgctt tgaagaatcg cccggagatc gtcaaggccc gagaaaccct ggagatctgc
     6061 gagctcgaag cgaagttcgc agataacgaa tacactcccg aactgaccaa gcggcagctc
     6121 aataatgctc tggcacaggc caggctcgcc gcaggccagg ctgagcgcgg agtgcgagtt
     6181 gaagcccggc agatgtatct gtcgcttgag gaatcccaca gggcgatttc aatcgcctca
     6241 gccgcgacca aacaggcaga ggaaaactac agaataatga agctccgtta cgagcacggc
     6301 atggaaatcg ccaattcact cttgggagcg cagttgagcc tcaccgaggc caggctctcc
     6361 gagcttcaat cgataatgag ctacaacatc gcccgactgc actttgatgc ctgggcaaac
     6421 tatccggccg aagaggctga gcctggtgat ccccgatgag ccagactgct caacacaaaa
     6481 gccagcagcg gcagacggct tttgtcacgg ttccgttcag ccgtagagct gcagacatct
     6541 gtatctgcgt tgtgatgtta ctcctcgccc ttgcaccatg cacctttgct gcggattctt
     6601 ctctaggcgc atcacacgcc gacgagagtc tggctctgac catggaagat gtaatgcgcc
     6661 tggccgaatc ctcctcgcca tcggtcgctt cggccaagcg cgctgtggag ctcgcaatgc
     6721 ttcagctcca ggctgccgaa gctcaaaagc ggccttctat aacggtccat gccgcacctc
     6781 tgcaaatagg gattgggccg ctaagtgtcc agagtgtcgt cgaagaagac agcaaaccga
     6841 tgggaaaagg cgagacgtcc atgtatgcgc tttcgcaagc tggcggagac gtgcggattc
     6901 cgctgggtgg cgggagcctt tcattgtcgg ccaccgcatc ctttgcattt tcagacccgg
     6961 ccttatcggg caaaagcgcc tggaacggca gtgcaagcct gtccctgccc atactcggcg
     7021 ctagctcccc gggcgaggcc ccaagctcaa caagctctgc ccagtctcaa ttgcggagtg
     7081 ctcagttcca gctcgatcag tcgatacgcc aagcgcgtgc ttcagcgcag gccgcatact
     7141 tcaatctcct tgcagcggaa gaccgagtcg ccgccgctga ccgcggtctc tcgcgtgccc
     7201 gcactcatgc ggctttcgtg gaggggcgat gttctgcggg gaacgcaagc gaacttgatg
     7261 cactagaagc aaaggccggc gccgacaggg ccgaagcggc agctcaatct gcccggcaca
     7321 atctggctat tgcttccatc agctttaacc gtgctatagg cagagacctg gaaactttag
     7381 tgcacctcgc gccggccgga gagtatgtga gatggcctga agacctggat ctcgacacct
     7441 gtacgcgggc ggcgctcgaa acgaggcccg aaattcccat ggcagagcaa gatgtcgcag
     7501 atgccgaggc tgccctcgct caagcgattg cggccaaacg cccggatgtc tttttccaag
     7561 gacaagcgtc atccggggga cactggcgcg tgggcctaga ggtctccgca ctgcttacta
     7621 gggattacgc tgcagagatc gccatcgagt ccgcaaaagg ccgagtggaa caggctaaag
     7681 caaatgccgt cgaaacaagg gattccatac tccttgaagt agttgaagca ttctacaatc
     7741 ttcgcgaagc agagtcatcg gtcgagctgg cgcgtttggc tgtcgccatc gccacggcaa
     7801 cattggacat aagagaggag caatgccgta ttggcgcagt cgcgtatgca gaagtctcag
     7861 aagctataga cgcagtgcgc aaagccgagt ccgatctcgc ctatcaactg gctgcatgct
     7921 tcatggcaaa gtccaggcta ttgcagtccg taggctccga caacatctaa gctttcccgt
     7981 ccatgtgcag ggcccgcgga cgctcttgcc tcagtatcca gggccctctt ttctttctgt
     8041 gcacaagctt tccgctttcc gcgcattact gtgacgatcg gccaactcgt ccaggatcgc
     8101 ctgcggagga atatcgtggt atgccaaaac gacgatggaa tggaaccaca agtccgccat
     8161 ctccctgatg atctcctctc tgtcgtcgtt cttggaagcc agcatcactt cgccggcctc
     8221 ctcagccact tttttcagca ctcggtcttg cccggagcgg atcaacttgg ccgtgtacga
     8281 accttccggt tggcttgcct ttctatcctc cactgtttcg tagagcttat ccagactgaa
     8341 cccggtccca gcagcatctg cactgcccca taactgcgta aagaaacagg actttcggcc
     8401 caagtgacaa gcctcgcccg tctggtcaac ctctatgagc aagcagtcgc ggtcgcagtc
     8461 gcagaagacc gcccgcactt tctggaaatg tccggaagtc tctcccttgg tccacagcct
     8521 ctggcggctc ctgctccaaa aacatgtccg gccttcacgg agcgtcattt cgatggactg
     8581 gcgattcatg tatccgacca tcagcacctg gccagtcaca ctctcctgaa tgacagcggg
     8641 aatcagtcca tctgcaccga atttgagcgt gtctatccat ccttccaggc tttcgccatc
     8701 atccgattcc aatcgttcaa tcataacctg acgtcaactc cttcccgctt gaggtgtctt
     8761 tttacatctc ccacgctcag cgttccgaag tggaagaccg aggctgcaag ggcagcatcc
     8821 gctccgcctt ccacgaacgc atcgcggaaa tgctcaggtg ttcctgcacc gcccgacgcg
     8881 atcacaggaa tgcccaccgc ttgggtcacg gcccgcagct gccgcaagtc gtagccgtcc
     8941 gtcgacccgt ctctatccac agacgtgagg aggatttcgc ctgcccccag ccgctcaacg
     9001 gtgcgagccc attcaatcaa atcgagatcc gtgcgttgct tgcccccgaa tgtgcacaca
     9061 acccacctgc cacagccgtc accattacca tcgttcttgc tgtcaacctc gatgcttacg
     9121 atgtcgttga catcacgatc gccatgtctg ttgcctgcat ccaaaaatgg ctccgcaatg
     9181 gcggatgcat ctatggcgga gacgatccgg tcaaccccga aagccttcgc cgcatgctgg
     9241 agcaactgag ggtttcgcac cgctgcagag ttgatgcaca cctttgacgc gccggcatcg
     9301 agaactcgcc cgacatcatc gaggcttgtg atgcccccgc ccgctgaaag gggaatcgag
     9361 acggcagacg cgacccggcc cacaagatcc agggcagtcc tgcgtccctc agcagtagca
     9421 gacagatcga gcaacatcag ctcgtcagct ccctggctct catagatctc agccaatcca
     9481 acggggtcgc ctgcatccct cagattctcg aagttaaccc ccttcaccac gcggccgtcc
     9541 ttcgtgtcaa ggcaaggaat gattctcctg cttcctgccg ctctcaacgc atctcggaat
     9601 ctgatcgcgc cagtgtacag agcgcggccg atgatgactc cgctcactcc ctggctttcc
     9661 aggcctttca gctgacgaag atcctggatc gaactgactc cgcctgcaac tataacgttt
     9721 gcgcctgccg acaacagctg agcgatggaa ttaacattaa ccccggccaa agtgccgtct
     9781 cgagacacat cagtacaaat gactgtctgg atcccagagt ccagggcgcg ctcgacaagt
     9841 tctgaaggag cgatgccgga ggctcgcacc catccatctg tcataactgc accgcagttg
     9901 gaatctatac tcaccacgat cctatcctcg ccaaatctcc taacaaggtc gcggagcatt
     9961 gccgggtcgc tacaggccgc agtccctagg atgactcgcg ccgctttagc ctcaagggcc
    10021 gattcgacgt ccttcggagt tctgaaacct ccgcccactt gtacaggaag accagtgtgg
    10081 gctatgatgt cgcgcacaac ctccaagttt accggcatac cttgccgggc tccatctaaa
    10141 tcgaccacgt gcagcatctg agcgcccatg ttcgcccatc gccgggcagt ttggcacggg
    10201 tcatcggaat actcggtgac tttcgcaaag tctccctgaa acaggcgaac acactttccg
    10261 ttgtggatgt ctatagccgg aagcactagc atcctacaac acccctttcg tggacggaat
    10321 cgaagacgtg tctcctgctg ccgcggcctg cctcaatgca agagccaccg ccttgaagac
    10381 agattctatg atatggtgcg tgttccaccc gtggtgcagg tctacatgca atgtgaactt
    10441 cccgttactt gccaaagccc gcatgaactc ttccaccagc tctgtatcga aatcgccaac
    10501 cttcgcgcat ggcatgggca cattgaaatg aaggaatgga cgccccgaca gatccacgac
    10561 ggccatgctc aaagcgtcat ccattggcac aagggcgctg ccaaagcgcg caatgccttg
    10621 cttgtcgcct agcgcttcgc agaatgcctc gccgatacag atgcccacat cttccacaag
    10681 atggtgatag tcgacttcga tgtctcccgt ggcatgaaga tccaagctaa actgggcgtg
    10741 aaatgccagc agcctgagca tgtgatcaaa aaatccgcag caagttgaga tgttcgcctg
    10801 atgacggcca gagcagccat ggtctctggc agcgtcgagg atcaacctca gctcgactga
    10861 tgtctccgat gtcttgcggt gcacttcggc cagtctcggt ttgggcatcc tactcaccac
    10921 cctcaatcct ttttccgtcg tcgtcacgaa gaccggcgga cgtttggttt gcttctgcca
    10981 acaccttctc caaagcctcc aggaatgcgt cgtcctcttc ctttgtccca cagctcaccc
    11041 tcaggcaccc gcgaagcatg ggctcatcgg gaaacacgcg aacagatacg ccataccgac
    11101 gaagagcatc tctgaccatc gttgcatctg gcatcgttgt cctaaacaga atgaaattcg
    11161 tacaagacgg gaaa
//
LOCUS       DAOSAI010000029        11098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3915_length_11098_cov_4.080866, whole genome shotgun sequence.
ACCESSION   DAOSAI010000029 DAOSAI010000000
VERSION     DAOSAI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3915_length_11098_cov_4.080866"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /gene="gcvT"
                     /locus_tag="PLM83_02095"
     CDS             <1..917
                     /gene="gcvT"
                     /locus_tag="PLM83_02095"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOK70231.1"
                     /translation="AAIDHLVTNDCSKAKIHQLVYSPMCYPDGGVVDDILVYKLCEDD
                     YWLVVNAANTDKDFDWVKRNVSGYDVTVENISPQVAQIAIQGPAAQRILQKTTPCDLS
                     SIKFFWCLPDVEIAGRKALVSRTGYTGEDGFEIYCRPEDARHIWDTLLAEGKDEGIVP
                     AGLGARDTLRFEVALPLYGHELSPEITPLEAGLGFFVKLKKPDFIGKDALAAQKEKGL
                     TRKIVGLELMGRGVPREGYAVMSDGKQVGYLTSGAFSPTMNKGLALALVAMEFTEIGT
                     ELDIDIRGKACPVKVVKTPFYQKSYRKE"
     gene            925..1308
                     /gene="gcvH"
                     /locus_tag="PLM83_02100"
     CDS             925..1308
                     /gene="gcvH"
                     /locus_tag="PLM83_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOK70232.1"
                     /translation="MSSKMRFTSDHEWVELEGNIATIGITEHAAEELGDVIYVELPAV
                     GDTFAQGDNLAVAESVKAAADIFAPIGGTVTEVNDALEDEPGLVNEDPMGNGWIVRID
                     VDDVTELDDLMDEEEYEEYLKEEEQ"
     gene            1308..2666
                     /gene="gcvPA"
                     /locus_tag="PLM83_02105"
     CDS             1308..2666
                     /gene="gcvPA"
                     /locus_tag="PLM83_02105"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOK70233.1"
                     /translation="MGSPHRYIPNTDQNVADMLADIGVSSIDDLFSVIPPEFRLTEPL
                     SLPKALSEPELSAELNRVAAKCAHSMQYTSYLGGGLYEHFVPSVVRHMLTRGEFYTAY
                     TPYQAEVSQGTLQAIFEYQTMICELTEMDVANASLYDAGTAVAEAAVMSCGATRRNKV
                     VVSRAINPRYRQVLRTYAHPRGIEIVTVDYDTQSGATPPESVPKDLLDDAACFIVQSP
                     NYFGIVEDMAALSEATHRSRALFVAVVNPISLGILAPPGSYGADIALGEGQPLGNPIS
                     FGGPLLGFFATTSKLMRKMPGRLVGQTHDDQGRRGYVLTLQAREQHIRRESATSNICS
                     NEALCALAATIYLSLMGPSGLRKVAELCTWNAHYLHDKIAKIPEYEFPFDGPFFNEFA
                     VRGRRPPAERNRRLLERGIIGGKMIEADYPELSGCTLWAATETRTRQELDTLIRELEV
                     LA"
     gene            2663..4132
                     /gene="gcvPB"
                     /locus_tag="PLM83_02110"
     CDS             2663..4132
                     /gene="gcvPB"
                     /locus_tag="PLM83_02110"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOK70234.1"
                     /translation="MKGPQPLIFELSIPGRTAVSLPEPAVPARELAELFPEEYLRKDP
                     PALPEVSEVDVVRHFVNLSQLNHAVDTGFYPLGSCTMKYNPKVNEDAARRPGFAAIHP
                     DQDESTVQGAMELLYTLEEYLCEICGMDNFTLQPAAGAHGEITGLFIINAYHESRNDK
                     RTKVLVPDSAHGTNPASAASAGMKIVQVKSDDRGNVDIEDLKAKAGPDVAALMLTNPN
                     TLGLFDEHIGEIAEIVHGAGALLYYDGANANAIMGKSRPGDIGFDVIHLNLHKTFSTP
                     HGGGGPGSGPVGVKKFLSPFLPAPVVRFNEATGKFFWDSDRPQSIGRVHSFNGNFGVM
                     VKTWAYIRSMGPDGLRAASEYAVLNANYIMRKLQPYYDLPYDRACMHECVLSGSRQKK
                     SADVKTLDIAKRLLDFGFHAPTVYFPLIVDEALMIEPTETESKETLDSFIDAMIRIAK
                     EAKDDPDVVREAPHDTPVGRLDETTAARKPILRYTAEEE"
     gene            complement(5340..5588)
                     /locus_tag="PLM83_02115"
     CDS             complement(5340..5588)
                     /locus_tag="PLM83_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70235.1"
                     /translation="MKAATESAPGIKPAVLGFIVRVSSCEANPIVYRRTYPHLGSGKS
                     GNGYLTLTVIEADSSTMRRIGERLIRRGSITTDPTIFS"
     gene            complement(5924..6853)
                     /locus_tag="PLM83_02120"
     CDS             complement(5924..6853)
                     /locus_tag="PLM83_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOK70236.1"
                     /translation="MLQGADQTVSSGEYDAMIIGAGPAGLSAAVNACVRKKSVAVISR
                     RLTSPSLEKAPWIENYLGVERISGRELSARFVNHARAAGAELIESDITGVFNLGDSFV
                     ALGSDGEFSAKAVVIATGSVQEASIPGESELLGMGVSYCATCDGPLYRGKKVVVLGYT
                     EHAIEEANFLAQICSEVTYVMARSVEPGDGPAIAEGIRVVKKRVLGIRGEDKVEEVEL
                     DGEAIPADGVFVIRDSIPVERLLEGVRVADGAIVVDRDMATNVPGVYAAGDCTGRPYQ
                     IAKAVGEGLVAGLSAARHVDRMAAKHLPKSLLL"
     gene            complement(6837..7691)
                     /gene="folD"
                     /locus_tag="PLM83_02125"
     CDS             complement(6837..7691)
                     /gene="folD"
                     /locus_tag="PLM83_02125"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOK70237.1"
                     /translation="MPATIIDGKAVAASIREDAAARAAEYREKFGRAPGLAVVIVGDD
                     PASRVYVNNKKKACAGCDIYSEEHALPATTTEDELLDLVRRLNADPKIDGVLVQLPLP
                     NHISEDKVIEAITPSKDVDGFHPINVGSLMVGKPALRPCTPAGIMELIHRTGIELKGK
                     RAVVVGRSNIVGKPVSIMLLAEHATVTICHSRTEDLPSVCREADVLVVAVGRPKMVKG
                     DWIKPGAVVIDVGTNRVGGKKLVGDVDFDEAVEVAGAITPVPGGVGPMTIAMLMKNCV
                     EAAFAARS"
     gene            complement(7846..9096)
                     /locus_tag="PLM83_02130"
     CDS             complement(7846..9096)
                     /locus_tag="PLM83_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70238.1"
                     /translation="MRREGYTRIRQPDASGSRPGLASLRHPRRLSFQSLPDISVHRVL
                     MAAILFFICGLVLSLGSIAEPTQTGNSWFLPTGSSPSAPVGPVLSLDIDITNDPGEEV
                     ATSHWTSPVEGYLELHGKAGTGGQGGYKLLARVDAGPVVGLEKIVLDCGYESSPSQES
                     HRTSDSKPERASESERTTSDSMCATLILVQANYDQSVGAMSKTTVSTAYAWNGRTLVE
                     VWSATTFSEAVWNLSWGAVGNVKANSWRWGVARERALRDFTSSGGIPATAAKGFDLGP
                     GNWVRLASASTVQFSGGPVPSVVMESLHEYHSYSEKDDAFKLITSRKVTQTFCWSDRW
                     KTFVLGEGTIGREGAIGMEYPGADDGLRIDPGTDVAILESERKCVEGLVFGPPFLLRV
                     KLPNGKRCFVSEDSVLTSAVESMV"
     gene            complement(9367..9759)
                     /locus_tag="PLM83_02135"
     CDS             complement(9367..9759)
                     /locus_tag="PLM83_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOK70239.1"
                     /translation="MNDRVWSIWICRARSNLARAKHGKFSEDVLYEDLAFDAQQAAEK
                     SLKALLLYLGMDAPRTHSIGYLLKLIEDGSQMAIPPHVQEAAVLSDYAVTTRYPGNYE
                     AVSEEEYLRAVELADDVCSWVMSIIRQC"
     gene            complement(9749..10051)
                     /locus_tag="PLM83_02140"
     CDS             complement(9749..10051)
                     /locus_tag="PLM83_02140"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOK70240.1"
                     /translation="MDVLSAIVKIIVSTADPDKIILFGSRASGDQSPDSDYDILVLKK
                     GDYHKRTLAQQLYRELAVIPAPIDLVVEDPDKAEQLRDVPGLIYGEALRGRVLYER"
     gene            complement(10442..>11098)
                     /locus_tag="PLM83_02145"
     CDS             complement(10442..>11098)
                     /locus_tag="PLM83_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOK70241.1"
                     /translation="LDGESLVLRCGADGVCKDTIVMERSDIKIPGMHNVRNVLAAIAL
                     CSAVGADPSAMRHAVSSFEGVEHRLEAVGEVGGVAYINDSIATTPTRTIAALNAVERP
                     IVLIAGGYDKNLPFDEMAEVALGKVHTVVLIGVTSDKIEKALVEAARRAESEPPRIVR
                     ARVFEEAVDAARASASPGDVVLLSPACASYGMFRNFEERGRTFKEIVGRYVEVAGCKE
                     "
BASE COUNT         2638 a         3164 c         2891 g         2405 t
ORIGIN      
        1 aggccgcaat cgaccacctg gtcaccaatg attgttccaa agccaaaatc catcagctcg
       61 tctacagccc tatgtgctat ccggatggcg gggtagtgga tgacattttg gtctacaagc
      121 tttgcgagga cgattattgg ctcgttgtaa acgccgcaaa tactgacaag gacttcgatt
      181 gggtgaaaag aaacgtctcg ggttatgatg ttacggtgga aaacatatcc ccgcaagtag
      241 cccagatagc aatccagggc cccgctgccc agagaatact acagaagaca acgccttgcg
      301 atctttcatc catcaagttc ttctggtgcc tgcccgacgt ggagatcgcc ggacgcaagg
      361 ccctcgtatc aaggacagga tatacaggag aggatggatt cgagatctac tgtcggcctg
      421 aggatgcccg ccacatttgg gacacccttt tggcagaagg caaggatgag ggcattgtcc
      481 ccgccggcct cggcgcacga gacaccctca ggttcgaagt ggctcttccc ctttacggcc
      541 atgagctatc acccgaaatc accccgctcg aagcgggcct aggcttcttc gtcaaactga
      601 agaaacccga cttcatcgga aaagacgccc tggccgctca gaaggaaaaa gggttgacgc
      661 ggaagattgt tggtctggag ctcatgggtc gcggagtgcc gcgcgaaggc tacgcagtta
      721 tgtcagacgg caaacaggtc gggtacttga cgtcaggcgc attttcgccg actatgaaca
      781 aagggcttgc actagctctg gtggctatgg agttcaccga aatcggcact gaactcgaca
      841 tagacataag agggaaagcg tgcccggtaa aagtggtaaa gacgccattt tatcagaagt
      901 cttacaggaa ggagtaaggg ggatatgagc agcaaaatga gattcaccag cgaccatgag
      961 tgggtagaac ttgaaggcaa catcgctaca atcggtataa ctgaacacgc agcggaggag
     1021 cttggagacg tgatatacgt cgaattgccc gcagtcggag acacattcgc ccagggcgat
     1081 aatctggcag ttgctgagtc ggtcaaggca gcagcagaca tattcgcccc tatcggcgga
     1141 acagtcaccg aggtaaatga cgcgcttgaa gatgaacccg gtctcgtgaa tgaagatccg
     1201 atgggcaacg gctggatagt gcgcatcgac gtagacgatg tgactgagct agacgaccta
     1261 atggatgaag aagagtatga ggagtaccta aaagaggagg agcagtgatg ggctcgccgc
     1321 acaggtacat tccaaatacc gaccaaaatg tcgccgatat gctggccgat atcggagttt
     1381 ccagcataga cgacttgttt tcggttattc cgcctgaatt caggctaaca gagcctttga
     1441 gcctgccaaa agctctttcc gagcccgagc tttcggcaga gctgaacagg gtcgctgcca
     1501 agtgtgccca ttccatgcaa tacacttcat acctcggcgg cggcctgtat gagcacttcg
     1561 tgcccagtgt agtccggcac atgctcactc gtggggagtt ctacacggct tacaccccgt
     1621 atcaggcaga agtgagccag ggtacgctcc aggcgatctt cgagtaccaa acgatgatct
     1681 gcgagttgac cgagatggac gtagccaacg catcgctgta tgatgccgga acagccgtgg
     1741 cagaagctgc tgtgatgtcg tgcggagcca cccgccgcaa caaggtcgta gtaagcaggg
     1801 ccataaaccc cagataccgc caggttctgc gcacatatgc gcacccccgc ggcatcgaga
     1861 ttgtgaccgt tgattacgac acccaaagcg gcgctacgcc tccagaaagc gttccaaagg
     1921 accttctgga cgatgcagcc tgcttcatcg tccagtctcc aaattacttt ggaatagtag
     1981 aggacatggc cgccctatcc gaagctacgc accggtcacg ggctctgttt gtggcggttg
     2041 taaacccgat atccttagga atcctggccc cgcccggaag ctacggcgcc gatatagccc
     2101 taggagaagg gcagccactg ggaaatccca tcagtttcgg cggaccgctg cttggattct
     2161 tcgcaaccac ctcgaagctg atgcggaaga tgccgggacg ccttgtgggc cagactcatg
     2221 acgatcaagg acgtcgcggt tatgtcctaa cactgcaagc acgcgaacag cacataaggc
     2281 gcgaaagcgc aacgagcaac atctgctcaa atgaagcctt atgcgccctt gcagctacga
     2341 tatatctgag cctcatgggc ccgtccgggc ttcgaaaagt cgcggagctg tgcacttgga
     2401 acgcccacta cctccacgac aagatagcca aaatccctga atacgaattc ccgttcgacg
     2461 gtcctttctt taacgaattc gcagttcggg gcagaaggcc accggcagag cgtaaccgca
     2521 gactgctgga aagaggaatc atcggcggca agatgatcga ggcagattat cccgagcttt
     2581 cagggtgcac gctctgggca gcaacggaaa cccgaacccg ccaagagctc gacaccttaa
     2641 tccgggaact ggaggtgcta gcatgaaagg cccgcagccg ctaatattcg aattgagcat
     2701 acccgggcgg accgcggtca gccttccgga gccggcagtt ccggcccgtg aacttgctga
     2761 actcttcccg gaagaatacc ttcgcaagga tcctccggcc cttccggagg tgagcgaagt
     2821 tgacgttgtg agacactttg tcaatctctc gcagctcaac cacgcggtgg atacaggttt
     2881 ctatccactc ggttcgtgta cgatgaagta taaccccaaa gtgaacgagg acgccgcgcg
     2941 aaggccaggc tttgcagcaa tccatcccga tcaggatgaa tcgacggttc agggagctat
     3001 ggagctcttg tacaccctcg aagaatacct gtgcgagata tgcgggatgg acaatttcac
     3061 cctgcagccg gcagccggtg cgcacggcga gatcaccggg ctcttcatca tcaacgccta
     3121 tcacgagtcg cgtaatgaca agcggacaaa ggtcttggtt cccgactccg cccacggcac
     3181 caaccctgcg agcgcggcaa gcgccggcat gaagatcgtc caggtcaaat cggacgacag
     3241 gggcaacgtg gatatagagg atctcaaggc caaggccggc cccgatgttg cagcattgat
     3301 gttgaccaac ccaaatacct taggcctctt tgacgagcac atcggagaga tcgccgagat
     3361 agtacacggc gcaggcgcat tgctctatta cgacggtgcc aacgccaacg ccatcatggg
     3421 caaatcccgc ccgggcgaca taggattcga cgttatccac ctcaacctcc acaagacctt
     3481 ctccacgccg cacggcggag gcggaccggg atcagggcct gtgggcgtca agaagttcct
     3541 cagcccattc ctgcccgcac cggtagtaag gttcaatgag gcaacgggca agttcttctg
     3601 ggatagcgac agaccacagt cgatcggccg agtccattca ttcaacggca acttcggcgt
     3661 catggtgaaa acatgggcct acattcggtc gatggggcct gacggcctgc gtgcagcgag
     3721 cgagtacgca gtgctcaacg ccaactacat catgcggaag cttcaaccgt attacgatct
     3781 tccgtatgat cgcgcgtgca tgcacgagtg cgtgctgtca ggatcgcgcc agaagaaatc
     3841 ggctgacgtg aagaccttgg atatcgccaa gcgcctgctg gacttcgggt tccacgcgcc
     3901 cacagtctac ttcccgttga ttgtagatga ggcgctaatg atagaaccca ccgaaactga
     3961 gagcaaggag acgcttgact ccttcatcga cgcgatgatc aggatcgcca aagaagcgaa
     4021 ggatgatccc gacgtcgtcc gcgaagcgcc gcatgataca ccggtcggaa ggctcgacga
     4081 gacgaccgcg gcaagaaagc cgatcctcag gtatacggcg gaggaagagt agcaaaacta
     4141 acagcactgt cccaccatgc agggcttgtg aattgcgggt gataagtata ggctgtagtc
     4201 ggccatgaaa cccgaagcgt tgcatggaca tgaggaagca aaagcctggt ttccgaatcg
     4261 gtgtagatac ccgacggata ccaggctttt tcggtgcact gcaagcagcc ttctgtaggc
     4321 tgacatcccc tctccacctt gggaaagctc attttcatct agctaggtgg atgattatct
     4381 tacgtcagac agagactccc gcttcacgtt tcaggtggca gcggatgtcg tcaccgtgac
     4441 ccaatggaag gtgagcgacg gacaactgaa ggccctaacg gcaatccaca atgatctggc
     4501 agatgccgtg agtgataact ggctcgagga tctgttttga cagattacgc gagctcgaga
     4561 ggcacaaaga ttcctcgcgt atatcttgag aatgctcggg gacgaactga accagaggaa
     4621 tggctgatcg atagcgaaca gcaaaatcta tttacatcat gctcccagag tattgttgcc
     4681 catactttct gcccaatgta gtgcacggtc agagtcgatg tggcgcttgt tttcgggcca
     4741 gcgaatgtgt ggcctgtggc cgaaataatg gtgaaggagc gcaactgcga gatagcgaaa
     4801 acctccaagc gtgagcccat acatgactgg taggcatatg tggcggcgtg tttcgtgatc
     4861 gaggagatgc ccgagagggt gcgtcgggag attgtagagg atagtcgata gacttcttat
     4921 aggtcgtgga tgcgaggttt tctggagtca cgtggttggc tgtgcggctc gggcaaggtt
     4981 agctgacatg ccggtgggtg ggctgtggac atcagcaggg gtgtatagca gaggggtgta
     5041 tgctgggtga agtatggttg cgcatgccaa ttaatgtaat acagcgaatg gttcgtagtg
     5101 ttttttgcta cactacgaat atttcgtgga tacgattaca tttataggta atgtgtggaa
     5161 agtaatgtgt attggggtat gccaaggtcg aattgacccg tttcggggtc ggatggtttc
     5221 catcttagtc caataagata cagagtattc catatgtcgc ccagataaat caaggtgctc
     5281 tcacattatg ttaacttata ctttgaccct acgatttttt cgtagcataa gaaaaaacac
     5341 tacgagaaaa tcgtagggtc agtggtgatc gacccacgcc tgatgagccg ttcaccaatt
     5401 cgtcgcatag ttgacgagtc tgcctctata actgtaagcg tcaaatagcc attccccgac
     5461 ttcccagacc ctagatgcgg atatgttcgt ctgtagacta tggggttagc ttcacacgaa
     5521 ctcacacgaa caatgaagcc gaggaccgca ggctttatcc ccggcgcact ctcagtcgca
     5581 gctttcactc tgcctttgag ttttggcgag atgacccata tagctatcgt agatctgagt
     5641 aagttcgctt acgtcgatgc tgcgctatgc ttcgcatgac gcgtgcacct ttgagtttgt
     5701 cgggtttgtg ggttttggag gacaaagtgc gatgcacgtt aaaggctatc gggaatatgg
     5761 aagtgtggtg tgccgttgtg gcggtaatgg gagataaaac ctccgatctt ggtgctttgt
     5821 gcctgaccgc cagtggctgt gctgcaggca tcagaataag cttttgacca agtcataagg
     5881 gatgtctcgg cgtccctttt gagccatggt tgcccggcct tcgttagagc agcagacttt
     5941 ttgggagatg cttggccgcc atccgatcaa catgtctcgc agcggaaagc cctgctacaa
     6001 gaccctctcc cacagccttc gcaatctggt aaggcctacc cgtacaatcg cctgccgcat
     6061 acaccccagg cacattcgta gccatgtcgc ggtcaaccac tatcgcgcca tcagcgacgc
     6121 ggacgccttc caagagcctt tccactggaa tcgaatccct gatcacaaag acaccgtctg
     6181 caggaatagc ctcgccgtct agttcgactt cttccacctt gtcctcgccg cggatgccga
     6241 gcactctctt cttcacaacg cgaatgcctt cggcaatagc cggcccgtct ccaggttcca
     6301 cgctgcgcgc catcacatat gtaacctcag agcagatctg ggcaagaaag ttggcttcct
     6361 ctatagcatg ctcagtatag ccaagaacaa cgaccttctt cccacgataa aggggtccat
     6421 cgcatgttgc gcagtagctc acgcccattc ctaaaagctc cgattccccg ggaatagatg
     6481 cctcctggac tgagcctgta gctatcacga ccgccttggc gctaaactcg ccgtcagaac
     6541 ctaaagccac aaaggaatca ccgaggttga acacgcccgt gatgtccgat tcgatcagct
     6601 cggccccggc agcgcgcgca tgattcacga aacgagcgga aagctcgcgc ccggagattc
     6661 gctctacccc aaggtagttt tcgatccaag gcgccttctc gaggctgggg ctcgtgagcc
     6721 ttcgcgatat taccgccacc gacttcttgc gcacacaggc attgaccgca gcagaaagcc
     6781 ctgcagggcc cgccccgatg atcattgcgt cgtattctcc ggaagacacc gtctgatcag
     6841 ctccttgcag caaacgcggc ctccacacag tttttcatca gcatcgctat agtcatcggg
     6901 cccacaccgc ccggcacagg agtgatagct ccggccactt caaccgcctc atcaaaatcg
     6961 acatcgccca cgagcttctt tccgccaacc ctgttggttc cgacatctat gaccacagcg
     7021 cccggtttga tccagtctcc cttaaccatc ttcggacgtc ctactgccac caccagaaca
     7081 tcagcttccc tacatacaga aggcaagtcc tcagttcggg aatgacaaat cgtaacggtg
     7141 gcgtgttcgg caaggagcat tatcgaaacc ggcttgccca ctatgttgct ccttccaaca
     7201 accacagcgc gcttgccctt caattctatg ccggtgcgat ggataagctc cattatcccc
     7261 gcaggagtgc acgggcgaag cgcaggcttg cccaccatca ggctgcccac attgatcgga
     7321 tggaagccgt cgacatcctt tgagggggta atcgcttcaa tgaccttgtc ctcggaaatg
     7381 tggtttggaa gaggaagctg cactagcacc ccgtcaatct tcggatcagc atttaacctt
     7441 ctaaccaaat cgagcaactc gtcctctgta gttgttgccg gcaaggcatg ttcctcagag
     7501 tagatgtcac agcctgcaca ggctttcttc ttgttgttca cataaacacg ggatgctggg
     7561 tcatctccaa cgatcacgac tgcaagcccc ggagcgcgtc cgaacttctc acgatattca
     7621 gcagccctcg cagcggcatc ctccctaatc gatgcggcaa cggctttgcc gtctatgatg
     7681 gttgcaggca tcagatcaac tccccatttt ctttctgcaa cttgcaagtt gccataatga
     7741 tcccctatcc cccacacgca tgggggggaa tatagcggtg ttatgccagt acggccagga
     7801 ttccctaaag ccataactgg caaacaacca ttcgcttgga ttatattata ccatggactc
     7861 aacggcagat gtgagcacgc tgtcctcgct gacaaaacag cgcttcccgt tagggagttt
     7921 gacgcgtaga agaaacggcg ggccgaacac aagtccctcc acacacttgc gttcggactc
     7981 caaaattgca acgtcggtgc cgggatcaat cctgaggcca tcgtcggcgc cggggtattc
     8041 catgccaatg gccccttccc ttccgatagt accctctcca agaacgaacg ttttccatct
     8101 gtcgctccaa cagaatgttt gagtgacctt gcgggaggtt atgagcttga acgcgtcatc
     8161 tttctcactg taggaatggt attcgtgaag cgactccata acaaccgagg ggaccgggcc
     8221 acccgaaaac tgcacagtgg aagcgctagc cagacgaacc caattgccgg ggccaaggtc
     8281 gaaccctttc gccgccgtgg caggaatccc ccccgaactg gtaaagtcgc gaagcgccct
     8341 ctcccttgcc acgccccatc gccaggagtt ggccttcaca tttccgaccg ctccccagga
     8401 caagttccat acggcctcgg agaaggtggt cgccgaccac acttccacca gcgtccgccc
     8461 attccaggca taggcagttg ataccgttgt cttgctcatt gcgcccacac tctggtcata
     8521 gttggcctgg acaaggatga gagtcgcaca catggaatca ctagtcgttc gctcggactc
     8581 agacgcgcgc tctggcttcg aatcggatgt cctatggctc tcttgactcg gagacgattc
     8641 gtaaccacag tccaacacga tcttctcaag tccaactacg gggccggcat ccacccgcgc
     8701 cagaagcttg tagccgccct ggcctcctgt tcccgccttg ccatggagtt ccagatagcc
     8761 ttccaccggc gaggtccaat gggatgtggc cacctcctcc cctggatcgt tggttatgtc
     8821 aatatcaagg ctgagaacag gtcctaccgg cgctgacgga ctgcttcctg ttggtagaaa
     8881 ccagctgttt cctgtttgag tcggctccgc tatgctccct agggagagga cgagaccgca
     8941 tatgaagaac agaatagccg ccatcagaac cctgtgaacg cttatgtccg gcaaggattg
     9001 gaaggacaat cgacgtgggt ggcgcaaaga tgctagcccg ggccgtgaac ctgatgcatc
     9061 aggctgtcga atgcgagtat atccttctcg gcgcatgtga tacgccccct acacgataag
     9121 actgagtaaa tcctactcct gtcgggggcg aatgattcat cgatgattgc ttcggaaaca
     9181 ttgacgatgt caggcgccgt catgcccaac ctgcaggctt tcgacgccga ccggcattac
     9241 aatccgtctt tgcatttgaa caacctagcg ttctgccatc cgtatttgga aaaccgagat
     9301 cacttgcgtc atcacagcat gtattactgc ctgcggcacc gcattcttgc aacaaaggcg
     9361 cgctgctcag cactgcctga tgatggacat gacccaactg catacatcat cggccaactc
     9421 gaccgcgcgc aagtattcct cctcacttac agcctcataa ttccccggat acctagttgt
     9481 taccgcatag tctgacaaga ctgccgcttc ttgcacgtgt ggaggaattg ccatctggct
     9541 gccatcttcg atcagcttca gcagataacc gatggaatgt gtacgcggag catccatccc
     9601 cagatagagg agaagcgcct ttaggctttt ttctgcagcc tgctgtgcat caaaagcaag
     9661 atcctcgtac aacacatcct ctgagaattt cccgtgcttt gcgcgcgcaa gattgcttct
     9721 agctctgcag atccaaattg accacactct atcgttcata aagaacacgc cctctcagag
     9781 cttcgccata tatgagtccc ggaacatcgc gcaactgctc tgccttgtcg gggtcttcca
     9841 caaccaagtc gataggagca ggaatcactg ctagctcgcg ataaagctgc tgcgcaagtg
     9901 tccgcttatg gtaatcaccc ttttttagga ccaatatgtc atagtcactg tcagggcttt
     9961 gatcaccact ggccctggat ccaaacaaaa tgatcttatc gggatctgca gttgatacaa
    10021 ttatctttac tatcgccgaa aggacatcca cagtgtttca cctccgcaca atccacgtta
    10081 agtggccggt gcttttgcgg ggtatggtcc gattcgacaa ggcatgactt ccccacccga
    10141 aagggaatgt cctccacaat cgcgtgcccc atctggcaag aggcccggaa tatgctggcg
    10201 atgattttat cgtggaaacg tacagcgtac gctcaatttt gcacgatccc ttggtccaat
    10261 tgagcagcac aagctcagaa cacgagcaat gcaaactgaa aactcgcgcg gcaacttatc
    10321 acaaaattta ctcagatgct cggccgcgct ccggccggat aatacaactc tctgcctacc
    10381 cggcctcgca ctcatggcgc ttgccaagat tccgccccaa tctcagcgct tcctgccgac
    10441 gtcattcctt gcatccggca acttccacgt atctcccgac aatctccttg aatgttcgac
    10501 ctctctcctc aaagtttcgg aacatgccat agctagcaca ggccggcgag agcagaacaa
    10561 catctccagg cgaagctgat gcgcgcgctg cgtccactgc ctcttcgaac accctggccc
    10621 tgacgatcct cggcggctcc gactctgccc tgcgtgcggc ctccacaaga gccttctcta
    10681 tcttgtcgga ggtgactcca ataaggacca cagtatgaac cttcccgagc gccacctctg
    10741 ccatctcgtc aaaaggcagg ttcttatcgt agcctccagc gatgagcact atgggtcgct
    10801 ccaccgcatt cagtgcggca attgttcgcg tcggcgtagt ggcaatggag tcgtttatat
    10861 aggcaacacc acctacctcg cccaccgcct caagcctgtg ctcgactccc tcgaaagagc
    10921 tgacagcgtg tcgcatcgct gatggatcgg cgcctacagc gctgcataaa gcgatggcag
    10981 ccagcacatt cctgacattg tgcatgccag gaatcttgat atcggacctt tccataacta
    11041 ttgtatcttt gcagacccca tcggcaccgc atcgcaatac caatgactcg ccatccag
//
LOCUS       DAOSAI010000030        11013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_3954_length_11013_cov_2.770944, whole genome shotgun sequence.
ACCESSION   DAOSAI010000030 DAOSAI010000000
VERSION     DAOSAI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11013
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3954_length_11013_cov_2.770944"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..1059)
                     /locus_tag="PLM83_02150"
     CDS             complement(274..1059)
                     /locus_tag="PLM83_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOK70242.1"
                     /translation="MYVAGVDVGSTAVKAVVLLDGRIAGTNVISTGTDQEKTAFQSLS
                     AAAQDAGVVDIEDISAMVATGYGRSMVKMADRTVTEITCHARGGRAFAEAAHVIVDIG
                     GQDSKVIRLDEDGMVADFVMNDKCAAGTGRFLEVMAERLEVPLSDFGKMWRATRKPAM
                     ISSTCTVFAESEVVSLLSHGVAREPIIRGLCDAVADRLAGMVHRVGLATEVVMTGGVS
                     RNDGVRLSLERKLGLKVSVPSMSQFAGAYGAAIIAFELAEKGR"
     gene            complement(1066..2349)
                     /locus_tag="PLM83_02155"
     CDS             complement(1066..2349)
                     /locus_tag="PLM83_02155"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOK70243.1"
                     /translation="MGPRGTRSVGNSLQVRAFRRFARKPWCYGAAKQVVDIMERRGWK
                     PYQISAIRCVMDDFRQAYTGERPCIYTTAFVPTELVYGLGAVPFLPEVAAGYAAAFGF
                     AYDALGAAEAMWYSADLCSFHRAGVGAGKLGVLPRPSAVIATSHLCDGGKKSLHRMSK
                     DAGCPFYLLDVPYDRSERGQKRLRDQVEHIAFDLPEKVPGISPEGMAEAIRVSNATLD
                     DYLEVCALRQHSPSPWRGGEALNYVVMFLGAWGNPWLAEFYKDLASHIRGIIASGEYP
                     CCSERHRLLWLNLRPYYKTGLLDYLELELETSVAFEEYNWPYWSELDPDDPFASVARK
                     MLANFGWGPIERRLAAVDRMIDDYSIDGVVQFAQWGCRQSNGGASMLADHLKRRGIPF
                     LNLGGDGVDVRNGSEAQAMTRLGAFVELIDAKKAR"
     gene            complement(2339..3451)
                     /locus_tag="PLM83_02160"
     CDS             complement(2339..3451)
                     /locus_tag="PLM83_02160"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOK70244.1"
                     /translation="MLLTSCSYIPLELPLAAGLPAKRLFVLDGAEVDADGLLPRDFCP
                     YARALMREYDPGDVLAIAGSCDAMRRAYDAIRYWGLAADVFYLDVPRTADEAAVDYFA
                     GELRQMARHIGVVGPAGQVEEQSLRLACEAMNKARAAMAETARSLEEGTLRASRLLQA
                     IMDVNELMGDVARWGMTWDREEGFGREVCDALTRAVRAEIEKALSVSVQPPALAGRVR
                     VGVSGTCLLDLSVVECIEEAGLEPVFIDSCMCQRVYDFSVDVNPSYDIYMELARSYLS
                     KPGCPRMFTGDERIARLGALAAMYGAKGLVYFAPKFCDLGYYDFPDVKRGLRESFGLP
                     SLLLEGEYGAGRTGQVLTRAVAFREMLEGRGEPSGA"
     gene            complement(3581..4438)
                     /locus_tag="PLM83_02165"
     CDS             complement(3581..4438)
                     /locus_tag="PLM83_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70245.1"
                     /translation="MMWDGAFGALLAGMIVAASLKGVTEMLLRTVGPVANYRRKPVAF
                     PGGLVVPFGAVLGPILVGALGGIHVDEPGRVGAYLLVIVGFGFAGFIDDMLGGHAQSG
                     FSGHFGALSQGSFTTGVVKVFLGGAVSLGAVELLAMAPVGIRDLMDAAVVALSANLIN
                     LLDRRPGRAIKGFLLGFAALSMWFWMKDGKAGLARMLSPAGSALGAAIAFFPADLGET
                     AMLGDTGSNALGAALGYCLLQMTVGWRTSALIVLVWLTLASERMSFGKVIEDNRVLRF
                     IDRLGREKE"
     gene            complement(4885..5583)
                     /locus_tag="PLM83_02170"
     CDS             complement(4885..5583)
                     /locus_tag="PLM83_02170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK70246.1"
                     /translation="MHPRVAIVPALNEEPSVGQSVAALLQCSAADCVIVVDDGSRDRT
                     SARAKEAGATVIRMAKTVGKGAAVATGIARARKMAPALLVFADADLGPSAAEMASVVS
                     AVVEGRGDMAVAGFPQPISSGGFGLVVGLAKRGVRQLAGLDLKWPLSGQRALLAEPFY
                     EVFAGDGAHMPRLDRGFGLEVGLAIDWARAGYTIVEVPTNMTHRETGKDLAGICHRAR
                     QFVEVLEALTMRRR"
     gene            complement(5583..6395)
                     /locus_tag="PLM83_02175"
     CDS             complement(5583..6395)
                     /locus_tag="PLM83_02175"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HOK70247.1"
                     /translation="MSLRYHVVSLAAVLVALGIGILLGAAISGGEGLAAQQRTLIDRL
                     DADFYRLSQDRDALAQELDAARRYAEESVPYMIRGSLAGKTVGIVSMPDVDRGDLESA
                     RDVCEGAGARVGAVVELADVALAGANSGQLSVWAQAITSSDMDRIKSLSTQGLAKIRM
                     AERCQFSVVLALSGPAEAKEAAFLQALSAAAKSAGTPLVVGWTRAAVLKVRSDVAAGK
                     FSSQLGKALPAAEVYGVGTAPGNVAMALALAGARGVYGQPPAPAALPAQEGR"
     gene            complement(6429..7619)
                     /gene="steA"
                     /locus_tag="PLM83_02180"
     CDS             complement(6429..7619)
                     /gene="steA"
                     /locus_tag="PLM83_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HOK70248.1"
                     /translation="MGYDGVDGKQSIGAEDSAGNARVRIRGTARADRKTKNLISRLRP
                     GEIAVIAHADIDEVSGRALVEACPKMVINADRSITGRYRCLGPGILLDAGIPVLDGVG
                     PHLLEVVADGDALEIEGNKVFKEGKPIGVGVWVTRDSLEAAYIEAEENLDAELEKFVV
                     NTLQYAIKEKDIILGDIDIPPLETQIHGRHCLIAVRGPRYREDLDAVASYIREMAPVL
                     IGVDGGADALVERGFAPDIIIGDMDSVSDETLFLGAELVAHSYPDGRSPGAERLANLG
                     LSHKTFVCPGTSEDIAMLLAYEKGASLIVAVGTHSNMLDFLEKGRPGMGSTFLVRLKV
                     GSVLVDAKGVSQLYRGRLRIGHIIGILLAAVVPLAAIALFSGSVADTARLIYLKIRVM
                     LGLL"
     gene            complement(7683..8492)
                     /gene="spo0A"
                     /locus_tag="PLM83_02185"
     CDS             complement(7683..8492)
                     /gene="spo0A"
                     /locus_tag="PLM83_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOK70249.1"
                     /translation="MSQGLVRLVMVDDNVDLVEVVTDFVSTQPDMSMCGVAFNGSEAI
                     SVIREVAPDVILLDLVMPHLDGLGVIEYVNSTPLSPHPRILVITAIGLETTAAGVVAA
                     GADYYMAKPFDLEVLARRIRRLAALPPERPQELQSCASCPQSTDLQRSIYDVEVSKTL
                     HRMGVPANLKGYLYLKDAIIMALERGHGAGSMTYSVYPTIAEAHGTTPSRVERAMRHA
                     IETAWNRGDIDFINRIFGHTVDAEKGRPTNSAFIARVASKVRIDLAGQIIS"
     gene            complement(8557..8814)
                     /locus_tag="PLM83_02190"
     CDS             complement(8557..8814)
                     /locus_tag="PLM83_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOK70250.1"
                     /translation="MPQDRPELDSSAALALEKLSPREREIIGMVALGLSNQAIGEKLF
                     ISDKTVKNYVTSIRRKLGVENRIQVALYAIRCGLVDPAIDQ"
     gene            complement(8963..10279)
                     /gene="spoIVB"
                     /locus_tag="PLM83_02195"
     CDS             complement(8963..10279)
                     /gene="spoIVB"
                     /locus_tag="PLM83_02195"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HOK70251.1"
                     /translation="MRAYSSKRIAIAFAVIVGFLSLAAIGAAVARIYVSPIYIRVFEG
                     QTFSVDMGPLGRGAVVVDPPDSLLMDAAIGSKVEVTPTKAGRTRLEVRLFNAIPVKRL
                     VVDVVPQMRVIPGGHSIGILLASRGVRVMGMYPVVGSDGLRYMPARDGGIEVGDMIVS
                     VDGYEVREPRQLETLVSEGALGGRPVRMGIVRDGREFDVTVLPAPADGGYRIGVYVRD
                     NAAGVGTLTFWHPGTMRYGALGHVVVDSQTGKEIPITDGRIVSARVSGIDLSGAGRPG
                     EKIGTFAGARDNVGVVEANTEVGIYGTLDAPLPNPYYADPIPVALAAAVHEGPAEILT
                     VVTGSSIERLEIEIVKVAKQSRIAGKGIVIRVVDPRLLELTGGIVQGMSGSPIIQDGK
                     LVGAVTHVFVSDPSRGYGVFAEHMLNVAGLMEGSERAAGWIVLLAA"
     gene            complement(10583..>11013)
                     /locus_tag="PLM83_02200"
     CDS             complement(10583..>11013)
                     /locus_tag="PLM83_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOK70252.1"
                     /translation="CDARELPDIYSGKADAVLVDAPCSGLGVLGRRPDARWRRSPDDI
                     LELSELQEEILDAAARCVKPGGVLVYSTCTVGTRENRDQVQRFAVRHREFVPSPIAPY
                     MPSAAARAFCSDDGHMIELLPGISGTDGFFISRFYRKRDI"
BASE COUNT         2453 a         3676 c         2658 g         2226 t
ORIGIN      
        1 tattctccgt ccacacttgc tgcacacgac gtagtcttcg agcagagtct caaagctgtc
       61 ttctataatc tcgacatatg gcttgccgca ttcgcatgag aatttatgct cttgttccac
      121 cttgcaacaa ctcccaaaac aatttgagtc atcggaacca tttcagccta cgcccacctg
      181 cgcgcgaaaa gattgccagt caatcccggc aaaactccac tcgagggggt gcggcgaatc
      241 acgcttccag tttactaacg cgctccgccc tcttcatctc cccttctcag ccaattcgaa
      301 ggcgattatc gcggccccat atgcccccgc aaactgagac atagaaggaa ccgacacctt
      361 caagccaagc ttgcgctcca aggaaagcct cacaccgtcg ttgcggctaa caccgccagt
      421 cattaccact tccgtggcca gccccactcg gtgcaccatg cccgccagac ggtctgccac
      481 ggcgtcacac agccccctaa tgatcggctc ccgggccaca ccgtgcgaaa gcaagctcac
      541 aacctccgat tccgcaaaaa ccgtgcatgt tgaggagatc atcgccggct tcctcgttgc
      601 gcgccacatc ttgccgaaat ccgaaagggg aacttccagc ctttcggcca taacctcgag
      661 gaaccggccg gttccagcag cacacttgtc attcataacg aagtcggcaa ccatcccatc
      721 ctcatcgagg cgtatgacct tggaatcctg gccgcctata tcgacgatta cgtgggcggc
      781 ctcggcaaaa gcccttccgc ctctggcatg gcaggtgatc tcagtgaccg tcctatccgc
      841 catcttcacc atggaccgcc cgtatcccgt tgcaaccatt gctgagatat cctcaatgtc
      901 aaccaccccg gcatcctgcg ctgccgctga aagagattgg aacgcggtct tttcctggtc
      961 agtcccagtg gatatgacat tggttcccgc aattctacca tccaaaagca ccaccgcctt
     1021 aacagcagtg gagcccacat cgacgcctgc cacgtacacc ctttgctacc tcgccttctt
     1081 cgcgtctata agctcgacaa aggccccaag acgggtcatc gcctgtgcct cacttccatt
     1141 gcgcacatca accccatcgc caccaagatt gagaaagggg attcctctcc tcttcaagtg
     1201 atctgcgagc atagaagcgc cgccattcga ttgcctgcat ccccactgag caaactggac
     1261 cacaccatca atggagtagt cgtcaatcat cctatcgacc gcagcgagcc gcctctctat
     1321 cggaccccat ccgaagttcg ccaacatctt ccgagcaacg ctggcgaagg ggtcatcagg
     1381 atcgagctcg ctccagtatg gccagttgta ttcctcaaag gccactgaag tttcgagttc
     1441 cagctcgaga tagtctaaaa gaccagtctt ataatatggt ctgaggttga gccaaaggag
     1501 cctgtgcctt tcgctgcaac acggatattc gccgctggcg ataattcccc gaatatggct
     1561 tgccagatcc ttgtagaact ctgcaagcca gggattcccc catgccccga ggaacatgac
     1621 cacgtagttt agggcttccc ctcccctcca gggagacggg gaatgctgcc gcagcgcaca
     1681 cacctccaaa taatcgtcga gggtcgcatt ggacacccgg attgcctcgg ccataccttc
     1741 cggcgagatc cccggaacct tttccggaag atcgaacgca atatgctcta cttgatcccg
     1801 cagacgcttc tgtccccgct cagatcgatc gtatggcaca tcaagtagat agaacggaca
     1861 gccggcgtcc ttgctcatgc gatgaagaga cttcttgccc ccgtcgcaaa gatgtgatgt
     1921 agcaatcact gcagagggac gcggcaggac gcccaatttc ccggcgccaa ctccggccct
     1981 gtggaaactg cacaagtccg cagaatacca catcgcctcg gctgctccga gagcatcata
     2041 ggcaaagccg aaggctgccg catagcccgc agcaacctca ggaagaaagg gaaccgcgcc
     2101 aagcccgtac acgagctctg taggcacaaa agccgtcgta tatatgcacg gcctctctcc
     2161 ggtgtaggct tggcgaaagt catccatcac gcaccgtata gcgcttatct gataaggctt
     2221 ccagccgcgc cgctccataa tgtcaaccac ctgtttagcc gcgccatagc accatggttt
     2281 cctggcaaat cggcgaaatg ccctcacctg aagtgaattg cccacgctcc gcgtgcctct
     2341 aggccccact tggctctccc ctcccctcca acatctcccg aaaggccacc gccctggtga
     2401 gcacctgccc cgtccttccc gcgccgtatt cgccctccag aaggaggctg ggcaacccga
     2461 aactttccct cagccctcgc ttcacatcgg gaaaatcata gtagcccaaa tcgcagaact
     2521 tcggagcgaa gtacacaagg cctttggccc catacatggc cgcaagcgcg cccaaacgcg
     2581 caatacgttc gtcgcctgtg aacattctag gacatccggg ttttgacaga tacgacctcg
     2641 ccaactccat atatatatcg tagctcgggt taacatcgac agaaaagtcg tacaccctct
     2701 gacacatgca ggaatcgata aagactggct ccagtccggc ttcctcaatg cactctacta
     2761 cggacaaatc gagaagacac gttcccgaca cgccgacccg cacccgtcca gccaacgctg
     2821 ggggttgcac cgagactgac agcgccttct cgatctcggc gcggaccgct cgcgttaaag
     2881 cgtcacaaac ctctcggcca aacccttctt ccctatccca ggtcattccc caacgcgcca
     2941 catcccccat aagctcgttt acgtccataa ttgcctgcaa tagccgggaa gcgcgcaagg
     3001 tgccttcttc caaagacctt gcagtctccg ccatcgcagc cctcgcctta ttcatcgcct
     3061 cgcacgcaag gcgcaagctc tgctcctcca cctgccctgc tgggcctacc accccgatgt
     3121 gcctcgccat ctgccgcagt tcgccggcaa agtagtccac agcagcttca tctgctgttc
     3181 gcggcacatc gaggtaaaaa acgtccgctg caagacccca gtacctgatt gcatcgtatg
     3241 cgcgcctcat cgcatcgcaa gaccctgcaa tggccaacac gtcaccaggg tcatattccc
     3301 tcatcaacgc cctggcatat gggcagaaat cccggggaag aaggccgtcc gcatctactt
     3361 cggccccgtc taggacaaag agcctcttcg ccggtagccc ggctgcaaga ggcagttcca
     3421 gcggtatgta gctgcacgac gtcaacaaca ttctcattcc tccggcccgg ttcgcacacc
     3481 ctagtaccat cccacacggc ggcctctgat tctgcttttt gtcgtgttct acgcaatgcc
     3541 cggactttcc tgcctcgatg cagccaggtt ccaggaacgc tcattctttc tccctcccca
     3601 gccggtcaat gaagcgaagc accctgttgt cctctatgac tttgccgaaa gacatccgtt
     3661 ccgatgccaa cgtaagccac acgagaacga tcagagcgct cgtccgccac ccgactgtca
     3721 tctggagaag gcagtaacca agggctgcgc cgagggcatt ggacccagtg tctccaagca
     3781 ttgctgtttc gccaagatcc gcagggaaaa aggcgatcgc ggctccaagg gcagatcccg
     3841 ccgggctgag cattcgcgcc aaaccggcct tcccgtcctt catccaaaac cacatgctaa
     3901 gggccgcaaa ccccagtaga aaccctttta ttgcgcgccc tggccgccta tcaagcaagt
     3961 tgatcaagtt ggcagagagt gctacgactg cggcgtccat caagtccctg attcccacgg
     4021 gagccattgc cagcagctcg acagcgccaa gagaaaccgc tccgcccaag aagaccttga
     4081 ccacccccgt agtgaaggag ccctgggaca atgcgccgaa atgtccgcta aaccccgact
     4141 gggcgtgacc tcccagcatg tcgtcgataa aaccggcgaa cccaaatccg acgatcacca
     4201 ggaggtaggc tccaactctg cccggctcgt ccacgtggat cccgccaagt gctccaacga
     4261 gaatgggtcc tagaacagct ccgaatggca caacaaggcc gcccgggaag gctaccggtt
     4321 ttcgacgata gttggccaca ggtccaacgg ttcgcaagag catttctgtg acgcccttca
     4381 aggacgcggc cacgatcatc ccggccaaca acgccccgaa agctccgtcc cacatcatcc
     4441 gtgcctccga agcaatcacc tcccagcgtt cctagggcta tctttcgcca ccgcccaaac
     4501 gaatatcacg acgagcacct ccgcgctcgt gtcagatgga ccgttcactc tgtccgaaaa
     4561 aatgagaagc tcgccgcggt cgcgccccca catggcgcaa ggcgcggcga gtcacatgca
     4621 gagtccccgc aagaatggct ccggctagca ctggaaaaca cccacgagct cgtgggctga
     4681 tctgtcttga ctctaatcag gcggggaacg agaatgggca tccaccgacg aaggtgtaat
     4741 gggtgcaaga gatgccccag aaaaggccgg tgaataaaag tacccaaacc ttggagagct
     4801 cactacgcga cgacgcaaac ttcttctggc gcgtccctac acgatgcatc cacgcccgct
     4861 cggagcatgt cgtctgccac acgctcagcg gcgccgcatc gtgagcgcct caagcacctc
     4921 tacgaattga cgcgccctgt gacaaattcc agccagatcc ttccctgtct ccctatgagt
     4981 catattcgtg ggaacctcaa caatcgtgta tcccgccctt gcccaatcta tcgccaagcc
     5041 gacctcgagt ccgaaccccc tgtccaacct gggcatgtgg gcgccgtccc ctgcaaacac
     5101 ctcatagaac ggctccgcca gtagcgcccg ctgcccggac aaaggccact tgagatccaa
     5161 gcctgccaat tgcctgactc cgcgctttgc aagcccgact accaggccaa agccgcctga
     5221 actgatgggc tgcgggaatc ctgccaccgc catgtctccc ctgccttcaa ccacagcaga
     5281 cacaacggat gccatctcgg ctgcgctcgg tcccagatcc gcgtcggcaa aaacgagaag
     5341 cgccggagcc atcttccgcg ctctggcgat ccccgtagct acagccgcgc cctttcctac
     5401 agttttcgcc atccggatca ccgtcgcccc agcctccttt gccctagcag aggtgcgatc
     5461 acgagatcca tcatccacaa cgatcacaca gtccgcagcg gagcattgga gaagcgcagc
     5521 cacgctctgg cccacactgg gttcttcgtt gagcgcgggc actattgcta ccctcggatg
     5581 cactaccttc cctcctgcgc gggcagggcc gccggtgctg gaggctgtcc gtataccccg
     5641 cgggcacctg caagcgccag cgccatcgcc acattgccag gcgcagtccc aacaccgtaa
     5701 acctcggctg cgggcaacgc ctttccaagc tgactcgaga acttgccggc tgccacgtcc
     5761 gaccggacct tgagcacagc agcgcgcgtc catccaacta ccaggggcgt tcctgcactc
     5821 tttgcggcag cagacaaggc ttgaagaaag gctgcttcct ttgcctcagc aggccctgac
     5881 agagccagca ccacgctgaa ctggcacctt tcggccatcc tgatcttggc gaggccttgg
     5941 gtcgacaaac tcttgatcct atccatgtca gaggacgtga tagcctgagc ccaaacactc
     6001 agctgtccgc tgttggcgcc cgctaaagcc acatcggcca actcaacgac cgccccgact
     6061 cgagcccccg caccttcgca aacatccctg gcagattcca gatcgcctcg atcgacgtct
     6121 ggcatggaca cgattcccac tgtcttgccg gccaaagacc cacggatcat gtatggcacg
     6181 ctctcttccg cataccttcg cgcggcgtca agttcctgtg ctaaggcgtc acggtcttgt
     6241 gacaacctgt agaagtcggc atcgagcctg tcaatcaagg tcctctgttg cgccgccaat
     6301 ccttcaccgc cagatatggc agccccaagc agaatcccta tccctagagc gacgaggacc
     6361 gccgccaaag ataccacgtg ataccgcaga ctcatcacgg caatctcccc atccgatcgg
     6421 ctttcggttc aaagcaggcc caacatcaca cggattttga ggtagatcaa cctcgccgtg
     6481 tcagccacag atccagagaa caaggctata gcagccaggg gcacaaccgc tgccaaaagg
     6541 atcccgatta tgtgccctat ccgtagcctt cccctgtata gctggctcac tccctttgcg
     6601 tccaccagta ccgatccaac cttcagccgc acaagaaacg tgctgcccat cccaggcctg
     6661 cctttctcga ggaaatcaag catattggaa tgagttccca ccgccacgat gagggaagct
     6721 cctttctcgt aggcaaggag catggctata tcttcgctcg tgccgggaca gacaaaagtc
     6781 ttgtgcgaaa ggccgagatt ggcgaggcgc tcggcgccgg gggatcggcc gtcaggatac
     6841 gaatgggcca ccaactccgc gcctaagaaa agcgtttcat cggatactga atccatgtct
     6901 cctatgatga tatccggcgc gaatcccctc tctactaggg catcagcgcc tccgtcgacg
     6961 ccgatgagga caggggccat ctccctgatg taggaagcaa cggcgtcgag gtcttctctg
     7021 tatctagggc ccctcaccgc gatcaggcag tgtctgccgt gaatctgggt ctccaacggg
     7081 gggatgtcta tgtccccgag gattatgtcc ttctccttga ttgcatactg aagagtattg
     7141 acgacgaatt tctccagctc ggcatcgaga ttctcctcgg cttcgatgta ggcggcctca
     7201 aggctgtccc ttgtcaccca caccccgacg cctataggct ttccctcttt gaaaacctta
     7261 tttccttcta tttccagggc atctccgtcc gcaaccactt ccaaaagatg agggccgacc
     7321 ccatccaaca ccggaatgcc cgcatccagc agaatcccag gacccaggca cctgtatctg
     7381 ccggtgatgg aacggtcggc atttatcacc attttagggc acgcttcaac caaggctcgt
     7441 cccgacactt catctatatc ggcatgggct atcactgcaa tttccccggg tcggagccgg
     7501 gaaattagat tcttcgtttt acggtcagct ctggctgtcc ctctgatgcg aacacgggcg
     7561 tttcccgcgg aatcctccgc tccaatgctc tgtttgccat cgaccccatc gtaccccata
     7621 cacttattga ttccccgagc tgcgtaggcc attcgccaga gtctaggagc acaatgacac
     7681 aatcagctga tgatctgacc tgccaggtct atccttacct tgctggctac acgggctatg
     7741 aaggcagaat tggttggacg ccccttctca gcgtcgacag tatggccaaa tatccgatta
     7801 atgaagtcga tgtccccgcg attccacgcc gtttcgatcg catgcctcat ggcccgttcg
     7861 accctggagg gtgttgtgcc atgagcttcg gcgatcgtcg ggtagactga gtaggtcatg
     7921 cttcctgcgc cgtgccccct ctccaaggcc atgatgatcg catccttcag ataaaggtac
     7981 cccttcaggt ttgcaggcac gcccattcta tgcagagtct tgctaacctc cacatcgtag
     8041 atggaacgtt gaaggtccgt gctctgcggg catgatgcgc aggattgaag ctcttgaggg
     8101 cgctccggcg gcaatgctgc gagccgccgg atccgccgcg ccaatacttc aaggtcaaat
     8161 ggtttcgcca tgtaataatc ggctcccgcc gccactaccc cggcggcagt ggtctccaga
     8221 cctatcgcgg tgattaccag gatgcgagga tgggggctca ggggtgtgct gttgacatac
     8281 tctatgaccc ccaacccgtc aaggtgcggc atgacgagat cgagcagaat tacatcgggc
     8341 gcaacctccc tgatgacact tatcgcttcg gagccgttga aagctacacc gcacatcgac
     8401 atgtcgggct gagtcgaaac gaagtcggta acaacttcga cgaggtcgac attgtcgtcg
     8461 accatcacca accgaaccag gccttgggac attatccgca gcctccaaga acattgatac
     8521 tgtcactttc tacagcgctg agcccatctc ctgccttcac tgatcaatcg ctggatccac
     8581 taaaccacat cttatggcat acagcgcaac ctggatcctg ttctcaaccc cgagcttcct
     8641 gcgtatgctt gttacgtagt tcttgacagt cttatcggat atgaagagtt tttcacctat
     8701 ggcctggttg ctcaatccta gagccaccat tcctattatc tcccgctcac gaggagaaag
     8761 tttctccagc gcgagcgcgg cagatgagtc cagttctggt ctatcttgcg gcacaggcct
     8821 cacctctcaa ccccatcagg gcgtgtacac agacgaagca aacttgcttc tccctctggc
     8881 ggtacataat atgtggtgcc aaatctccct gtgtataggc tttttgacgg ggtctgcgat
     8941 tcggacccac atcgccgcct cctcaagcgg ccaacaaaac gatccaaccc gctgcgcgct
     9001 cgcttccttc catcaatccg gcaacgttca gcatgtgttc ggcaaaaacc ccgtatcctc
     9061 tcgaagggtc ggaaacgaag acgtgagtca cggcccctac gagcttccca tcctggatta
     9121 taggggatcc gctcattccc tgtactattc cgcctgtcag ctcgaggaga cgtgggtcaa
     9181 ccaccctgat gacaatacct ttccccgcga tccgactctg ctttgcaacc ttcactattt
     9241 cgatttccaa cctttctatg gaagacccgg tgacaacagt gagaatctcg gcagggcctt
     9301 catggaccgc cgcagccagc gcaaccggaa tcggatccgc gtagtaggga ttgggaagtg
     9361 gcgcatccag tgtcccgtat atccctactt cggtgttggc ctccacgacc cccacattgt
     9421 cccgcgcgcc cgcgaatgtc ccgatcttct ccccaggtct ccctgcacca ctcagatcta
     9481 tccctgatac gcgcgctgag acgattctcc catctgtaat tgggatctcc ttgcctgtct
     9541 gcgaatctac tacgacatgt cccaaagccc cgtacctcat cgtgcccggg tgccagaaag
     9601 ttagggtgcc cacgcctgcc gcattatctc tcacataaac cccaattcga tagccgccat
     9661 cggcaggcgc cggcaagacc gtgacatcga attcccgccc atcccgaaca attcccatcc
     9721 tcacaggcct accccccaat gcaccttcgc tcacaagcgt ctccagctgg cgcggctcac
     9781 ggacctcata tccatcgact gagacgatca tgtccccgac ctcgattccc ccgtcgcggg
     9841 ccggcatgta tctgagtccg tctgagccaa caaccgggta cattcccatc accctcaccc
     9901 cgcggcttgc gagcaggatg cctatggagt gtcccccagg aatcacccgc atctggggca
     9961 ctacgtccac aactaggcgt tttacgggga tcgcattgaa gagccgtacc tccagccgcg
    10021 ttcttcccgc cttcgtggga gtcacttcca ctttcgatcc tatggcggca tccatcagaa
    10081 gagagtctgg aggatcaacg actacggccc ctctgccaag agggcccatg tccacactga
    10141 atgtttggcc ttcaaaaacc ctgatgtaaa tgggagacac atagattctg gctaccgcag
    10201 ccccgatggc cgcaagcgaa aggaaaccga caatgaccgc aaaagcgatc gctatgcgtt
    10261 tcgacgaata cgcccgcatc cttccaccat ctcgccgcgg gcgtatctga cgcgcgcgta
    10321 cgcgccggcc cgcgcgggtt taacgtgtcc ggcacgagca aggcgtattc gcgctaaaag
    10381 atgctgtagt tggcaactag acttgatttg aaatcagact gaagaagaaa ccgattcccg
    10441 tgacgcttag tggatcaagc cacaacggca caagagtagt tgcgaaatgg agcatgcggc
    10501 agcgggatgc acgcccatga aaggcgatac gaacgtctga tagaaaaagc taacacatcg
    10561 aacagggatt tgcgtcctat tctcaaatat cccgcttcct gtaaaacctg gatatgaaga
    10621 atccatcggt gccagagata ccgggcagga gctcaatcat gtgcccgtcg tcagaacaga
    10681 atgcacgggc agcggctgag ggcatgtacg gggctatcgg cgacggcacg aactcgcgat
    10741 gacgcaccgc gaaccgctga acctggtccc gattctcccg ggttccaacg gtgcacgtgc
    10801 tgtacaccag cacccctccg ggctttacac acctagcagc cgcatcgagt atctcttcct
    10861 gcagctccga aagctccaaa atgtcgtctg ggctcctgcg ccaccgtgca tcgggtcggc
    10921 ggcctagcac acccaggcct gaacatggcg catcaacaag cacggcatca gccttgcccg
    10981 agtagatatc tggaagctct cgtgcatcgc agc
//
LOCUS       DAOSAI010000031        10690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4086_length_10690_cov_3.713775, whole genome shotgun sequence.
ACCESSION   DAOSAI010000031 DAOSAI010000000
VERSION     DAOSAI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10690
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4086_length_10690_cov_3.713775"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLM83_02205"
     CDS             complement(<1..334)
                     /locus_tag="PLM83_02205"
                     /inference="COORDINATES: protein motif:HMM:NF039908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HOK70253.1"
                     /translation="MNLLGFDFSNITLMQALTTIADIAIVAFLMYKVISFLKGTQAVS
                     LIKGLVVLFAANAISEYLKLEALHWLLRQATTMAIVGIPIVFQPEFRRVLEQLGRGRL
                     FGNPMKSLD"
     gene            complement(447..1238)
                     /locus_tag="PLM83_02210"
     CDS             complement(447..1238)
                     /locus_tag="PLM83_02210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOK70254.1"
                     /translation="MRLVAMDLDGTLLRSDKSISDAAIEAIREAHSRGVAFAVATGRM
                     FQTSQPVVDGLLDELPIIAYNGALIKYSNCGLTLLRLPIAARGGERVVRFMWEWGMVF
                     QAYIDDQLWVARVAPETVAYSKKYGAPINVMEDIEEFVNREAFKYLVLDDEERIADVR
                     KAIDGVLDPGLRTMLSSPGMIEIVRSDVSKGRALSHLASSMGISMDNVMAIGDSENDI
                     DMLRAAGIGVAMGNAGDDVKAEADYIVASNDDDGVVEALERFALL"
     gene            complement(1242..1637)
                     /locus_tag="PLM83_02215"
     CDS             complement(1242..1637)
                     /locus_tag="PLM83_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOK70255.1"
                     /translation="MAYHSNGQVGMPEWVIKWLINALALLIVSYVPYRGGTLLEIDGA
                     GAALIAAMVLGIVNTFIRPIFMMFSLPLRILSLGLFTLVINALMLCMGSWLMGNAFNI
                     PGFWAAIIASLLLSLVSSALNHLIRGDSR"
     gene            complement(1701..1892)
                     /locus_tag="PLM83_02220"
     CDS             complement(1701..1892)
                     /locus_tag="PLM83_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOK70256.1"
                     /translation="MQRRLYRSVRDRMLGGVCGGLANYLNVDPTLIRLLWVAFTLFSQ
                     APGLLLYVIAWIIVPEEPI"
     gene            complement(2036..2407)
                     /locus_tag="PLM83_02225"
     CDS             complement(2036..2407)
                     /locus_tag="PLM83_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70257.1"
                     /translation="MDQERKQVLEMVRDGKLTVDEAQQILDAMTYEEERARPAASSRR
                     NPRFLRIRVKDEDGSKVNLNIPISLAKVMWRFIPREAMNELSNQNIDIETILDAVHEG
                     AEGQLIHVKDEDGDEVEISVV"
     gene            complement(2463..3527)
                     /locus_tag="PLM83_02230"
     CDS             complement(2463..3527)
                     /locus_tag="PLM83_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOK70258.1"
                     /translation="MGEERKIVLNLLSEGKITVEEAEQLLAALEGEVESVDEADSVRV
                     ETISEGDLHGRLSKVFAEMVEAGQELPGRLSRAVESAFGGFGWSFRGVRGEKSFEEEI
                     CGPEINAIDFAANNGSIKLAVWEKPGFRVVVRATVVDADSRDEADRRLESVARLVVND
                     GKVSIDCEDKSILDSMSIEAYFPKDRKYDVFAHTRNGSITLDRLNGGHVEVCSSNGRI
                     ALDSIKVSTLAAETANGAVTASGHLGDGSIETSNGSVTAALAYESGGHLNVRTSNGSI
                     RIKVLNSSDVSYEVNAETKNGATRVLDEGAAIRSERRSTGGQVKKITVSAGDSALDDS
                     AKVTVDARAMNGSITVESCR"
     gene            complement(3559..3936)
                     /locus_tag="PLM83_02235"
     CDS             complement(3559..3936)
                     /locus_tag="PLM83_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOK70259.1"
                     /translation="MARREALGRCPVCDGILDVTRLTCTDCETVIEGRFDTCKFCQLS
                     SEQKNFIEVFIKNRGNIKEVEKDLGISYPTVRNRLDAVIAALGYAVDDSEDDVATRRK
                     EIIESLAKGEITADDAVRQLKGK"
     gene            complement(4076..4840)
                     /locus_tag="PLM83_02240"
     CDS             complement(4076..4840)
                     /locus_tag="PLM83_02240"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOK70260.1"
                     /translation="MEKDDGSITTSSCASPDRSAISVSSIGTSATEASAIIASASVAT
                     RIGIVSIEAGESGLSRVVLPRIDMETLKNLPTSPRIHPLFIDASVTLAQIGIDFPMCD
                     HSSICQMAASNSVPVYGSVSWDGVPPSEIVSAACRDIKLFFEGELDPGKLWFDYPLKL
                     PAGSDFAREVMVGMTRIAPGRVAAYGALAAAAGRPRAARATGAIVGANPLPLVVPCHR
                     VVGVGGRLTGYGGGLPLKVALLAMEQTIINRTGVLG"
     gene            complement(4945..6795)
                     /gene="uvrC"
                     /locus_tag="PLM83_02245"
     CDS             complement(4945..6795)
                     /gene="uvrC"
                     /locus_tag="PLM83_02245"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOK70261.1"
                     /translation="MALDDTLAVLPTSPGVYLMKDADGEVIYVGKAINLRNRVRSYFQ
                     TSSDHSPKVVMMVEHIADLDYIVTASELEALILECNLIKKYRPRYNVRLRDDKTYPFI
                     KVTTGEPYPGVYLTRRVVRDGSRYFGPYTDVSATRETLRLLKRVFPLRQCSKKIDPDS
                     PAPRPCLNYQIKRCLGPCAGQIDVEAYGEVVDEVVMFLEGRQDDLARSLKRRMVAAAD
                     ALNYEQAAVFRDQLQAVETVVQKQRIVSESGADQDVIAIARDQWGACAQILFVRGGKL
                     VGNDHFMLGGTDGADDAETLSSLVKEYYSQAEAGSLPREVLLESEIEDADIVAAWLSD
                     LRGRAVSVKTPKRGEKRGLVELASANAAEFLERKRAAEQREARARMDALAELSIHLRL
                     SKSPSRIECYDISTLQGTEAVGSMVVFEAGLPAKSQYRRFRIRQVEGQDDYAMMAEVI
                     RRRLVRAIGDDPDPKFARLPDLIVVDGGRGQLNAAADVLDSLGRGDIPLISLAEREEE
                     IYLREQPDPLILPRDSAALHMIQYIRDEAHRYALAYHRNLRGKRSISSELTDLPGIGK
                     KRTVALMKEFGSLEALTDATVDEIAATPGMNRVVAMTVYEHLHPDRRVDG"
     gene            complement(6782..9658)
                     /gene="uvrA"
                     /locus_tag="PLM83_02250"
     CDS             complement(6782..9658)
                     /gene="uvrA"
                     /locus_tag="PLM83_02250"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOK70262.1"
                     /translation="MSVEKIIIRGAREHNLKNIDLDLPRDRLVVVTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMNKPALDYIEGLSPAISIDQKAQGRNPRSTVAT
                     VTEIYDYMRLLYARIGIPHCPNCGREIAQQTVQQIVDRIMAMPEGSRIQVLGPVIRGK
                     KGEHRKALDDLKRDGYIRVRVDGEVMELSEEISLERYKMHSIEAVVDRLAVRPGIETR
                     LADSLETALKLGDGVVIIDVVGGKELVFSEKLACPDCGIGFGELEPRLFSFNSPYGAC
                     PVCSGLGVKIDFDPKLIFDMEHSIEDGAILPYNGNGVIADYYMGRVNQVATEKGIDTS
                     VPMSELPESAMEMIMYGCGSEKFRFRYVNSRGRTKYWESRFEGVIPNLQRRYKETTSD
                     YIRENLERFMSASPCTKCNGARLKPEALAVTVGGKSIAEVTSMSTVKALEFFEELGRG
                     LTERESLIARQILKEIGARLGFLTNVGLDYLTLHREAGTLSGGESQRIRLATQIGSSL
                     VGVLYILDEPSIGLHQRDNARLIDTLKDLRDLGNTVIVVEHDEEMIRSADYIVDMGPG
                     AGAHGGQVVAAGTVEEIEANPASITGQYLSGVRRIEVPAARRLPNGNALVIKGAREHN
                     LKNIDVTIPMGVFVCVTGVSGSGKSTLINEILYARLAHDLHRAMTRPGAHDEILGIEH
                     LDKVIGIDQSPIGRTPRSNPATYTGVFTHIREVFAKTPEARARGYKPGRFSFNVKGGR
                     CEACRGDGIIKIEMHFLPDVYVPCEVCHGARYNRETLEVKYKSKSISDVLEMTVEEAL
                     EFFANIPAIKRKLQTIYDVGLGYIKLGQPATTLSGGEAQRVKLATELSRRTNGRTLYV
                     LDEPTTGLHFADVQKLLDVLQRLVDGGDTVVVIEHNIDVIKTADYIIDLGPEGGDKGG
                     RVIAAGTPEEVAATPGSYTGEYLVKIAGVMPAQRELEAMTGGTR"
     gene            complement(9655..>10690)
                     /locus_tag="PLM83_02255"
     CDS             complement(9655..>10690)
                     /locus_tag="PLM83_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOK70263.1"
                     /translation="DESHVTVPQIRAMYNGDRSRKESLVQYGFRLPSAFDNRPLTFSE
                     FEERVNQVIFVSATPADYERTVSSQVVEQIIRPTGLVDPAVTVKPVRGQVDDLIGEIR
                     RTVDAGYRVLVTTLTKRMAEDLTDYLREIGIKVRYLHSEIQTLERVEILRGLRLGDFD
                     VLVGINLLREGLDLPEVGLVAILDADKEGFLRSDTSLIQTIGRAARNVDGRVIMYADQ
                     ITGSMREAIDETERRRSAQIQFNREHGITPESIKKEVRDLSEIQRVADRSVGSSAACG
                     GQSGVRGQGGVAAEFGCADVESMDERELARYIKALEDEMKKAAGLLEFERAAELRDTI
                     RDLRRRFVEL"
BASE COUNT         2452 a         3299 c         2432 g         2507 t
ORIGIN      
        1 gatcgaggga tttcataggg ttcccaaaca gcctcccccg acctagttgc tcaagtactc
       61 ttcgaaactc aggttgaaag acaattggta tgcctacaat agccattgta gtagcctggc
      121 gcaacagcca atgcaatgcc tcaagcttga gatactccga aatggcattt gcagcaaaca
      181 gcacgacaag gcctttgatc aaagataccg cctgagtgcc tttgaggaag ctgatgacct
      241 tgtacatgag aaacgccacg atcgctatat ccgcgattgt ggtcaatgcc tgcatcaagg
      301 ttatgttgga aaaatcgaaa ccaagcaggt tcatgagcaa acactcccga aacgggtatc
      361 gagactatac attacaacat ttcgttcttc gcgcgaatac accttcctac ataatgcaga
      421 acctcatgga agtcgagctt atctgttcac aaaagcgcga atctctccag cgcctccact
      481 acgccatcat catcattcga ggcaactata tagtcggctt cggccttcac atcgtccccg
      541 gcatttccca tcgccacacc gatgccggcg gcacgcaaca tgtcaatatc gttttctgaa
      601 tcgccaatgg ccatgacatt atccatgctt atgcccatgg aagaagcaag atgagataga
      661 gcgcgcccct tagatacatc cgatctgaca atctcgatca tgccagggct cgacaacata
      721 gtacgcaggc cagggtcgag caccccgtct atagctttcc tcacatcggc aatacgctct
      781 tcatcatcga gcaccaagta tttgaaggcc tcgcggttca cgaattcctc gatgtcttcc
      841 ataacattga tgggagcgcc atatttctta ctgtaggcca ctgtctcagg agcaacccgc
      901 gcaacccata actggtcatc gatgtaggct tgaaagacca tgccccactc ccacataaac
      961 cttaccacac gctcgccacc gcgggcggcg ataggcaggc gcaggagagt gaggccgcaa
     1021 ttgctgtatt ttataagagc accattgtaa gcgattatag gcagttcatc gaggaggcca
     1081 tccaccactg gctgcgatgt ttggaacatc cgcccggtgg ccacggcaaa tgcaacccct
     1141 cgcgaatgag cctccctaat agcctctatg gcagcatcag atatgctctt atcggatcga
     1201 agcaatgtgc catcaagatc cattgcgacc aaacgcatca actaacgact atccccccgt
     1261 atcagatggt tcaaggcgga gctcacgaga ctgagcaaca acgaagcgat tattgccgcc
     1321 caaaacccgg gaatattgaa cgcattgccc ataagccaag aacccataca cagcatcagg
     1381 gcattgatca ccaaagtgaa aagacccaga ctcagaatcc gcaggggcag actgaacatc
     1441 ataaaaatcg gccttatgaa ggtgttcacg atgccaagaa ccatggcagc tatcagggca
     1501 gcccctgctc catctatctc caagagcgtg ccgccgcgat acggaacgta tgagactatt
     1561 agcagtgcaa gggcattaat gagccatttt ataacccact ctggcattcc gacttgtcca
     1621 ttgctgtggt aagccattgt caaccctcca ttgcccccat gataggccgt aacctgacgc
     1681 ctgcaaagct ccgtctcaag ctatataggc tcttcaggca caataatcca ggcaattacg
     1741 taaagaagga gacccggtgc ctgactaaat aacgtaaaag ccacccatag caggcgtata
     1801 agagtaggat caacattcag gtagttggct agcccgccac atacaccgcc caacattctg
     1861 tcgcgaacag acctgtatag tctcctctgc attacatgga cacctccatt gcccggcgtc
     1921 ttgctgtatt cttcagcagg tatgcaattc cttcaccgca catatcccgt tccggacact
     1981 tcgcgcaagc aaacgctatc cgtgcaagat accctttacc cagcagtccc cgagctcaga
     2041 caacgcttat ctcaacctca tccccatctt cgtctttcac atgaatcagc tgcccctcgg
     2101 cgccctcatg caccgcatcg agaatggtct caatgtcgat attctgattt gagagctcat
     2161 tcatagcctc tcttgggatg aacctccaca tcaccttggc aagggatatg gggatgttca
     2221 aattcacctt tgagccatct tcgtccttta cgcgaatcct caagaacctc gggttccgcc
     2281 ttgaactcgc agcaggcctc gctcgttctt cttcatatgt catggcgtcc aaaatctgct
     2341 gcgcctcgtc aaccgtgagt ttcccatctc tcaccatctc gagtacttgc tttctttctt
     2401 gatccacaga gcgcacctcc cctcatatca ctctcatagc gcctgcaacg cggccaccgc
     2461 catcacctgc aagactcgac agttatggag ccgttcatgg cacgagcatc gactgtgacc
     2521 ttggcactgt catccaatgc agaatccccg gcagaaaccg tgatcttctt gacttgccca
     2581 cccgtcgacc ttctctcgga acgaatcgcc gcgccctcat caagcactcg cgtcgcccca
     2641 tttttggttt ccgcattcac ctcgtacgat acatcagagc tgttcaaaac cttaatccgt
     2701 atactgccgt tgctcgtcct gacgttcaaa tggccacctg attcataggc cagagcagca
     2761 gttacgcttc cattggaggt ctcgatgctt ccatcgccca aatgaccgga tgcagtaacc
     2821 gctccgttgg cggtttcagc tgcgagagtg gagaccttga tcgaatcgag cgctatacga
     2881 ccattggatg aacagacttc cacatggccc ccattgagcc tatctagcgt tattgatcca
     2941 tttcgagtat gcgcaaacac atcgtatttg cggtccttcg ggaagtatgc ctctatcgac
     3001 atcgagtcca gaatcgactt gtcctcacag tctatgctca ctttcccgtc attgacaacc
     3061 aatcttgcaa cagactcaag tctgcgatct gcttcgtctc tggaatccgc gtcaacaact
     3121 gtagctctga ctaccacacg gaatcctggt ttttcccaga cggccaactt tatactcccg
     3181 ttattggccg cgaaatcgat ggcatttatc tctggcccgc agatctcctc ttcgaaagac
     3241 ttctccccgc gcacgccgcg aaaactccat ccgaagcctc cgaacgccga ctccaccgct
     3301 ctggacaaac gcccagggag ttcctgccca gcctctacca tttcagcgaa gaccttggat
     3361 aaccggccat gcagatcgcc ctcggaaatc gtttccacac ggaccgaatc ggcctcgtct
     3421 accgattcaa cctcgccttc caaagcagcc aacagctgct ccgcttcctc cacagtgatt
     3481 ttaccttctg acagaagatt caggacgatc ttcctctctt cgcccatgga ccagacctcc
     3541 cccttttcaa ttagactcct acttcccttt tagttgacgc actgcatcat cagcagttat
     3601 ctcgcccttg gccaaagact caataatctc ttttctcctg gtcgcaacat cgtcctcgga
     3661 atcatcaacc gcatacccca atgccgcgat caccgcatca agccggttcc ggactgtagg
     3721 atacgagatt ccaaggtcct tctctacttc cttaatattg cccctgttct ttatgaacac
     3781 ttcgataaag ttcttctgct ccgaactgag ctggcagaat ttgcaggtgt cgaaacggcc
     3841 ctctatcaca gtctcgcagt ccgtgcacgt cagcctggtc acgtccaaga ttccatcgca
     3901 aacaggacat cgtcccaacg cctcccgtct tgccatgaca acacctcctt tttgcctccc
     3961 ttgcttcaca ttataaagct catcattaat gatgtcaatg ctgcgctaaa taattgtgct
     4021 attcttgcag atattcctcg gccgtacaaa cagggtctaa tattgtagac tttgctcatc
     4081 cgagaacgcc ggtgcggttg attatcgtct gttccatggc caacagtgcc accttgagcg
     4141 gaagtccccc gccatatccc gtgagcctgc cgcctacgcc aaccactcta tgacaaggaa
     4201 caaccagggg aagaggattg gcccccacaa ttgcgcccgt ggctctggca gcccttgggc
     4261 ggcctgcggc ggcggcgagc gctccgtagg ccgcaactcg cccaggagct atccttgtca
     4321 taccaaccat cacttcgcgg gcaaagtcgc ttcccgcagg gagctttagt gggtaatcga
     4381 accaaagctt ccccggatcc aactcgccct caaagaaaag ctttatgtcg cggcacgcag
     4441 ctgagacaat ctcagacggc ggcactccat cccacgatac actcccatat acaggtacgc
     4501 tgttagacgc agccatctgg caaatcgagg aatgatcaca catgggaaaa tcgatgccta
     4561 tttgcgcaag cgtaacactg gcatcgatga acaaaggatg gatccgtggg cttgtaggga
     4621 ggtttttcag ggtctccatg tctattcttg gaaggaccac ccggctcagg ccgctctccc
     4681 cggcctcaat cgagactatg ccgattctcg tagcaacact ggctgaagct ataatggccg
     4741 aggcctctgt agccgatgtc ccaatagatg aaacactgat ggcggaccga tccggcgatg
     4801 cgcacgaaga ggtggtaatg cttccatcgt ctttctccat tcacctgccc ctttcacttt
     4861 ttcaatcatc tcgttctgtt tcaaccaagg ctcagcgcta ggggcgacaa tcaggagagc
     4921 ccgagaaaag cggcatcata gaattcaacc atcgactctc ctgtcggggt gcaaatgctc
     4981 atatacggtc atcgcaacca ccctgttcat cccaggagtt gcagcaattt cgtccacagt
     5041 ggcatccgtg agggcttcca gcgaaccgaa ctctttcatc agagcgaccg ttcgcttctt
     5101 tcctatgccg ggcagatcag tcagttcaga gcttatgctc ctttttcccc tgaggttccg
     5161 gtggtacgca agagcatatc tgtgggcctc atcgcggata tactggatca tatggagagc
     5221 cgcagaatcc cgcggcaaga tcaaggggtc cggctgctct ctgaggtaga tctcctcttc
     5281 gcgctccgcc agactgataa gtggaatgtc gccccgtccc aggctgtcga ggacatcagc
     5341 ggccgcgttg agctgccctc gccccccgtc tacaactatc agatcgggaa ggcgtgcaaa
     5401 ctttgggtcc gggtcatcgc caatggctct cacaagcctt ctccgtatca cctcggccat
     5461 catggcgtag tcatcctgcc cctcaacctg cctgatcctg aaacgcctgt actgagactt
     5521 ggccggcaag cccgcttcga agacaaccat cgaccccact gcttcagttc cctgaagagt
     5581 cgatatgtcg tagcattcaa tgcggctcgg cgacttggaa agtctgagat ggatcgatag
     5641 ttcagcaagg gcgtccatgc gagcacgggc ctccctttgc tcagcagctc gcttccgttc
     5701 tagaaactcc gccgcattgg cagaagcaag ctcgaccaat ccccgcttct ccccccgctt
     5761 gggagtcttc acagatacag cccgcccccg aaggtcagac aaccatgcgg ccactatgtc
     5821 tgcatcttct atctcggact ccagtaaaac ctcacgggga agcgaccctg cctcagcctg
     5881 agaatagtat tccttaacga gggatgacaa agtctcagcg tcatccgcgc cgtcagttcc
     5941 acctagcatg aaatggtcgt tgccaacgag ttttccgcca cgcacaaaca agatctgtgc
     6001 gcacgccccc cattgatccc gtgctatcgc tatcacatct tggtcagccc cgctctccga
     6061 gactattctc tgcttctgca cgactgtctc caccgcctga agctgatcac ggaaaacagc
     6121 cgcctgttcg taattgaggg catcggctgc ggccaccatc cgccgcttga gactgcgggc
     6181 aagatcgtcc tgccgtcctt caagaaacat cacaacctcg tctacaactt caccgtatgc
     6241 ttcaacgtcg atctgcccgg cacatggacc aaggcacctc ttgatctgat aatttaggca
     6301 aggacgcggt gcgggagaat ccgggtcaat cttctttgag cactgtcgta gcggaaagac
     6361 ccttttcaga aggcggaggg tttcgcgcgt cgcagagaca tccgtgtaag ggccgaaata
     6421 acgagacccg tcacgcacaa ccctcctcgt caagtatacg ccagggtagg gctcccccgt
     6481 cgtcaccttg atgaaaggat acgtcttatc gtcacggagc ctaacattgt atcttggtct
     6541 gtatttcttg atcaggttac actcgaggat cagtgcttcc agttcggacg ctgtcacaat
     6601 gtaatccaaa tcggctatgt gctccaccat catgacaacc tttggcgaat ggtcagaaga
     6661 agtctgaaag tatgatctca ccctgtttct gaggttgatg gctttgccga cgtagattac
     6721 ctccccgtcg gcatccttca tcagatacac gcccggactc gtgggaagaa cagccaaggt
     6781 atcatctagt gccacctgtc atcgcctcca gctcgcgttg ggcaggcatt actcccgcta
     6841 tcttcaccaa atactccccc gtgtacgacc ccggggtcgc cgccacctcc tcgggcgtgc
     6901 cggcggcgat cacccgtccg cccttgtcgc cgccttcggg ccccagatct atgatgtagt
     6961 ctgcagtttt tatcacatcg atattgtgtt caatcactac gacagtatcg cctccatcca
     7021 cgagacgctg cagaacatcg aggagcttct gcacatcggc aaagtgcaag cccgtcgtgg
     7081 gctcgtcaag cacgtagagg gtccttccat tggtgcgcct cgacagttct gtggcgagtt
     7141 taacccgctg agcctcgccc cccgaaagcg tggttgccgg ttggcctagc ttgatgtagc
     7201 caaggcctac atcgtagatg gtttgcaact ttctctttat cgcaggaatg ttcgcaaaga
     7261 actccaaagc ctcctcaaca gtcatttcga gcacgtcaga tatgctcttt gacttgtatt
     7321 ttacctccag agtctcccga ttgtagcgtg ctccgtggca gacctcgcat ggcacgtaga
     7381 catcgggcaa aaaatgcatt tctatcttga tgatcccatc tccacgacac gcttcacacc
     7441 ttccgccctt gacgttgaag ctgaaacgac cgggtttgta tcccctagcc ctagcctcag
     7501 gcgtcttggc aaagacctcc ctgatatgcg taaaaactcc ggtataggtt gccgggttcg
     7561 atcggggcgt ccgaccgatg ggagactgat cgatgcctat gaccttgtcg agatgctcga
     7621 tgccgagaat ctcatcatgc gcgcccggtc tggtcattgc gcgatgaagg tcatgtgcca
     7681 aacgcgcata gagtatctcg ttgatcagtg tgctcttgcc cgaacccgag actcccgtaa
     7741 cgcatacgaa aacccccatg ggtatagtga catcaatatt cttcagatta tgttcgcgag
     7801 cgcccttaat gacaagcgcg tttccgttag gcaatcgtct ggctgccgga acttcaatac
     7861 gccttacgcc cgacagatac tggcctgtta ttgatgcggg attagcctct atctcttcaa
     7921 ccgtgcccgc ggccaccact tggcccccgt gtgcgccggc cccgggcccc atgtcgacga
     7981 tatagtcggc cgaccgtatc atctcctcat cgtgttcaac aactatgacg gtattgccca
     8041 ggtcacgtag gtctttcaag gtatcgataa gacgagcatt gtcgcgctgg tgaagcccga
     8101 tagacggctc gtccaggata tagagaaccc cgaccaagct cgagccgatc tgggtagcca
     8161 gcctgatccg ctgggactca ccgccagaca aagtcccagc ctcgcggtgg agggtaagat
     8221 agtcgaggcc tacattggta agaaacccga gccttgcacc gatctccttg agaatctgcc
     8281 ttgcaatcag cgactccctc tctgtaagcc ctcttccgag ttcctcaaag aactcgagcg
     8341 ccttcacagt ggacatcgac gtgacctcgg caatggattt tcctcctaca gtcactgcga
     8401 gcgcctccgg tttgagcctg gcaccattgc actttgtaca tgggctcgcg ctcatgaacc
     8461 gctccaaatt ttcccttatg tagtcagacg tcgtctcctt gtagcgcctc tgaaggttgg
     8521 gtatgacccc ttcaaacctc gattcccagt atttagtgcg ccctcgtgag ttaacatagc
     8581 gaaacctaaa tttctcgctc ccgcacccat acatgatcat ctccattgcg ctctctggaa
     8641 gctccgacat gggaaccgac gtatcgatcc ccttctcggt agccacctgg ttaaccctgc
     8701 ccatgtagta atcggctatt acgccgtttc cgttgtatgg aagaatagcg ccgtcttcaa
     8761 tcgagtgttc catgtcgaag atgagtttgg gatcgaaatc tatcttcact cctaacccgg
     8821 aacagacagg acatgcgccg tatgggctat tgaatgagaa gagcctcggc tccagttcac
     8881 caaagcctat cccgcaatca gggcacgcca acttctcaga gaagaccagc tcttttccgc
     8941 ccacaacatc gatgatgacg accccgtctc caagcttgag cgcggtttcg agcgaatccg
     9001 caagcctagt ctcgatccct ggccttaccg ccagcctgtc cacaacagct tcgatgctat
     9061 gcatcttgta tcgttcaaga gagatctcct cggaaagctc catcacctcg ccgtccacac
     9121 gcacacgaat gtacccgtct cgcttgaggt cgtcaagggc cttccgatgc tcgccttttt
     9181 tgcccctgat aacagggcca agcacctgta tccgcgagcc ctccggcata gccatgatcc
     9241 tgtcgacaat ctgctggaca gtctgttgcg ctatttccct gccgcaattg gggcaatggg
     9301 gaatccctat cctagcgtaa agaagcctca tgtaatcgta aatctctgta acagttgcca
     9361 ccgtcgaacg cgggtttcgg ccctgcgcct tctgatcaat ggagatagcg ggcgaaagcc
     9421 cttcgatgta gtctagggcg ggcttgttca tctgtcctaa gaactgtctg gcgtaagcgg
     9481 acagagactc cacataccgc cgttgccctt cggcatatat ggtgtcaaaa gccaacgatg
     9541 acttgccaga gcctgacaac cccgtaacga ccacaagacg atctcgcggc aagtcaagat
     9601 cgatgttctt taagttgtgc tcccgcgctc ccctgataat tatcttctct acgctcatag
     9661 ctcgacgaac ctccgtctga gatcacgaat agtgtctctt aactcggcag cgcgttcaaa
     9721 ctcgagaaga cccgccgcct tcttcatctc atcctcaagt gccttaatgt atcgagcgag
     9781 ctctcgctca tccatggatt ccacatctgc acagccgaac tcagcggcaa ctccaccctg
     9841 tcctctgacc ccagattgcc cgccacaggc tgcactgctg ccaacggatc tgtctgcgac
     9901 ccgctgaatc tcggatagat cgcgcacctc ttttttgata gattcaggag ttatcccgtg
     9961 ctctctgttg aattggatct gcgctgaacg gcgccgttca gtctcgtcga tagcctcgcg
    10021 catagacccg gttatctggt cagcatacat tattacacgt ccatctacgt tgcgagcagc
    10081 acgtccaata gtctgtatca gcgatgtatc tgacctcaaa aacccttcct tgtcggcatc
    10141 gagtatggca acgaggccta cctccggaag atccaacccc tccctgagta agttgatccc
    10201 aaccagcaca tcgaagtcgc ccaggcgaag acctcgcagg atctccacgc gttccaatgt
    10261 ctgtatctcg gaatggaggt atctcacctt gatacctatc tctctgaggt aatctgtgag
    10321 atcctcggcc atccgctttg taagggtggt aacgagaacc ctgtatccag cgtccacagt
    10381 gcgcctgatt tcaccgatca aatcatcgac ctggccccgc acaggcttca ccgtgaccgc
    10441 cggatccaca agccccgtcg gacgaatgat ctgctccacc acttggctgc ttaccgtcct
    10501 ttcataatca gccggcgtag ccgagacgaa tatgacctgg ttcaccctct cctcgaactc
    10561 gctaaacgtg aggggcctgt tgtcgaaagc agatggaagc ctgaatccgt actggaccag
    10621 gctttccttg cgcgagcgat cgccattgta catcgcccgt atctggggca cagtgacgtg
    10681 cgattcatcg
//
LOCUS       DAOSAI010000032        10667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4098_length_10667_cov_4.271862, whole genome shotgun sequence.
ACCESSION   DAOSAI010000032 DAOSAI010000000
VERSION     DAOSAI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4098_length_10667_cov_4.271862"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PLM83_02260"
     CDS             complement(<1..990)
                     /locus_tag="PLM83_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOK70264.1"
                     /translation="MKRLASVLFIVSLLVLGSLGALAADDPLLFSGKIVTVDKYGNVL
                     TDISEEAVAASGAEVGDMVIVKIGDKTLVVPFVTIYGDVDRGTPLMRTTNNAIQFAIN
                     YGNFAQTYGASEGTEVSVYLKEKGAYLSEIQIRSLKKLENRDEYDSDEAFANFREVTA
                     GNIKPGILYRVSHPSLGDERSSYAARLVEEHGINTVLNLSDTPEEFEANLAHSEYYRQ
                     LNEKGNIVAVGMGVDFAAKEFTEKFKTVLEFMIDHDGPYAVHCVEGKDRAGLVIAVLQ
                     GLAGATVEEIVEDYMVSYENYYRLEKGTRGYELVSKIMYDQLREMNGGEAVTDV"
     gene            complement(1925..3163)
                     /locus_tag="PLM83_02265"
     CDS             complement(1925..3163)
                     /locus_tag="PLM83_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70265.1"
                     /translation="MDLLTNSLFLLSLAILCGHYLGKISLARFNLGASGTLFAGMGLS
                     WLIYDRFVAPYEIAVANGAIDVPSYAVRILQNGFVDKANFDFFLLLFVASVGLLAAKD
                     VARVFRGNGAKFLLLAISITTTGAGVAYSLFRLIGKLDMHAITGLYAGALTSSPGLGA
                     ALESAACMFPDPASAAARAAQASVGMGYAIAYPFAAITVIICMQLLPKLFDVDLQQEV
                     AQLDSDLANGHTWSASKPASFCVPGIAFVSLIGYILGSISIPLGSVGKVSLGTTGGVL
                     VTGLILGDRGRLRMDERALNVTKQVALYYFLAYVGLNYGYQAATAMTGPNAMLVLVGI
                     LTTATSVLVGFLFGRYVLKMNWIILSGAICGSMTSTPGLGAAIDATGRNEAAGGYGAT
                     YPVGLLCKVVIVQVLSKLVW"
     gene            complement(3211..4134)
                     /gene="sucD"
                     /locus_tag="PLM83_02270"
     CDS             complement(3211..4134)
                     /gene="sucD"
                     /locus_tag="PLM83_02270"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOK70266.1"
                     /translation="MSILIDEQTSVLVQGITGNQGRFHTQQMLAYGTNVVAGVTPGRC
                     GMEVEGVPVFDTVRAATEEYSIDASVLFTPAAAVKDAALEAMEAGIRTLVIITEHVPV
                     HDCLEIMAYADRIGATITGPNTYGIVSPGRCKIGIAPSSIFLPGSVGVVARSGTLSYE
                     IVGNLTASGVGQSTVVGIGGDRVVGQSFIDILRLFEADSATSVVVLIGEIGGSAEEEA
                     APYITQMTKPVVAYIAGQSAPPGKRMGHAGAIIERGRGTYHHKRRALEEAGVRVAELP
                     SQVTGIVQTELSMLHADPFYGLIWSFSRPQQ"
     gene            complement(4183..5298)
                     /gene="sucC"
                     /locus_tag="PLM83_02275"
     CDS             complement(4183..5298)
                     /gene="sucC"
                     /locus_tag="PLM83_02275"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOK70267.1"
                     /translation="MRLFEYMGKQLFIDFGIRVPRGMVCDTPKTAAQIAQRLGGPVMI
                     KAQVLSGKRGKGGGIRLADGPKESKLAAEQLLQSTVQGFPVELLLIEEKLAIGQELYL
                     SLIVDGVSKKPVLIASAQGGMDIENVPEEQIIRQPIDVELGLPTFLARDIVRRLKIVP
                     SSPCGKALTQVVTALYRLFTAIDAELVEINPLAICGDTVYAADAKITVDDASLYRHAH
                     IPTVSELTLGEQEASKLGLSYVDLDGEIGVMANGAGITMATLDMITYFGSKPANFLDI
                     GGGANQEATRKGLELVLAKQPKAVLVNIFGGITRCDEVAQAIVGIRRQYGKSIPMVVR
                     LAGTNQDAGRAILEKEAGIEVFDLMQTAAQRAVELAG"
     regulatory      complement(5480..5668)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5858..6574
                     /gene="fabG"
                     /locus_tag="PLM83_02280"
     CDS             5858..6574
                     /gene="fabG"
                     /locus_tag="PLM83_02280"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOK70268.1"
                     /translation="MRETVLITGASRGIGYATAELFAVNGYTVYANYRSSPKSLELLS
                     EKLSERGCTLIPLYADVSNSNDVEHMVSQTNGVDILINNAGIAQFKQFVDMSETDWDN
                     MMSVNLKSVYLCTHCVIPHMIKRKHGAIVNISSVWGVTGGSCEVHYSAAKAGVIGFTK
                     ALAKEMAPSGIRVNCIAPGVIETDMNKELSDYDRQLIKEQTPLGTIGLPDHIAQAALF
                     LAENQFITGEILNVNGGFHI"
     gene            complement(6924..7415)
                     /locus_tag="PLM83_02285"
     CDS             complement(6924..7415)
                     /locus_tag="PLM83_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK70269.1"
                     /translation="MVVRSAFDGDDEQLVALIAAFRVELAAFKQQESNPNQDAASTEL
                     ADYRSNRFRLAVAESDEGHVIGFIACRIEDDVVWAESLYVLPEYRRVGVASRLYGYAE
                     GIADELGSDTVYNWVHPNNAAIIAFLRKRGYDVLNLIEVRRKYAGECISGDVMVGSER
                     FRY"
     gene            7981..9144
                     /locus_tag="PLM83_02290"
     CDS             7981..9144
                     /locus_tag="PLM83_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOK70270.1"
                     /translation="MDYQSISNLKMLRYAKGLTQQELADAAGISRDAYRNIETGRSDP
                     KISTLRSIAKALDAEIDDLFVPIKKPAQIRFRSSKRMNTRDMVLFDVVKWMQDYNWLE
                     QALDEQTRWALSDLSTEMSALESGEKRAILCARKSRNRLGLDEEEPIRDICGLLESAG
                     VKVYPKEVMSEAFFGLSVGPDCGGPVVVVNVWDRISVERWIFTAAHELGHLLLHMDSY
                     DVDKLDENEAEEQEANIFASYFLMPEAVFQKEWQDTRGLPFLDRVLKVKRMFRVSYQT
                     VLCRLAETMNPRGNVWRKFQVDYKKRYGKTLKRADEPQPASTDKFMASYSESYRAHEP
                     ERLSETDFIPDRLARLVRQALEDEVISLSRAADMMGIDIQSMRNMTLSWADCP"
     gene            complement(9522..9872)
                     /locus_tag="PLM83_02295"
     CDS             complement(9522..9872)
                     /locus_tag="PLM83_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOK70271.1"
                     /translation="MDLSAAREELERKIAASSDPYEEQSVVLGYVDGAEVCLMGWAER
                     PLRRDVKDVPPHANSADSMQIVFEGGMEIYYPDEQTTVVMRPGDCHVVPAGKRHSCVS
                     AMKSVVLVVVGYRA"
     gene            complement(9981..>10667)
                     /locus_tag="PLM83_02300"
     CDS             complement(9981..>10667)
                     /locus_tag="PLM83_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HOK70272.1"
                     /translation="TWPYDIKEILELKDAATLYEYWVFIQVCNVVQDLVRTRPNQASA
                     ADVAFDANAIAAMDARVGVGESLGAQMNCDRLGARLARGIRVQFPGEIEVSYNREFRG
                     YSGWFRPDVTLITPAGIWVFDAKFRFDRQSESDLSAKADDIHKMHTYRDALSGCRGAY
                     AVYPGDAAIMYSAPTPACPTDVNPSVAGRGGIGSVANRVADGVGAIPLRPCSDVSSLS
                     SVIRSLLSLE"
BASE COUNT         2655 a         2927 c         2579 g         2506 t
ORIGIN      
        1 gacatcggtg accgcttcgc cgccattcat ctcacggagc tggtcgtaca ttatcttgga
       61 gactagctca taccctctgg tgcccttctc cagtctgtag tagttctcat aggagaccat
      121 gtaatcttcg acgatctctt cgaccgtcgc tccggcaagg ccctggagaa ccgctatgac
      181 caatccagcc ctgtccttcc cttccacgca gtggacggcg tacgggccat cgtggtcgat
      241 catgaactca aggaccgtct tgaacttctc ggtgaactct ttcgccgcga agtccactcc
      301 cataccgact gcaacgatgt ttcctttctc gttgagctgg cgatagtact cggagtgcgc
      361 gaggttcgct tcgaattcct ctggggtgtc tgacaggtta agcaccgtgt tgatgccgtg
      421 ctcttccacc agcctggctg cgtatgagga acgctcgtcg ccgagtgacg ggtgggagac
      481 tctgtacaag atgcctggct tgatattccc tgccgtgact tctctgaagt tggcaaaggc
      541 ctcgtcactg tcgtactcgt cgcggttctc aagtttcttg agactgcgga tctgtatttc
      601 agacaggtaa gcacctttct ccttcagata gaccgatact tcagtcccct cggaagctcc
      661 gtatgtctgt gcaaagttgc cgtagtttat ggcgaactgg atagcgttat ttgtcgttct
      721 catcaagggg gttccccggt cgacatcccc atagatagtg acaaagggaa ctacaagtgt
      781 tttgtcccct atcttcacga tgaccatgtc ccctacttca gcccccgaag ctgcaacagc
      841 ctcttcgctt atgtcggtta gcacattgcc atacttgtca actgtgacaa tcttgccgct
      901 gaaaagaaga ggatcatctg ccgcgagtgc gcctagcgaa cccaagacca aaagggaaac
      961 gatgaacaga acagacgcca gtcgcttcaa tcttaatacc tccgcttcgc ctgatttttg
     1021 ggagcttatg cctggaccgg gacttccttt ttcgaattga gatagatcca agccgcccca
     1081 ggaattgact ctagcaagtc ctattatagc atgataggcg gcgacatatc gggttttggt
     1141 cggcggcacc cggtagatgt tgcccaatta tctgccagat gtaggaccgg ggagaagcaa
     1201 gcgagcccgt ttgtgtgtta accatactag tgctcgcgct tagacgcgaa agggctgagt
     1261 ttcctttcca atgctctgtg cctcgataca ccagcatcaa tgttgcgctg ctcagcatgc
     1321 gtcaggtaaa atcatggaac aacccagcag ataaccgttg gcatttgggg atttgccatc
     1381 tatatcgggc atcacagtga aatgcctaaa cttcccgatg tatcacatga tgggtcgaaa
     1441 ggtcgatctg tatacctgga acttcgatcg catctaagat caagcaggtt ctcgggcttc
     1501 actacgagtc agattgcgcc aaatacgagt ttggccggga ctccccttgc attctccaaa
     1561 gggtttggct ctagcaaaat caacagcgca ggtgaatgat gcgaaagtag aaattaactc
     1621 caaattctcg caaggtctcc gatcccagtg actctgtaaa cttcacgggt ttcaacagcg
     1681 tagctgctgg atggcggctg aaggtgagtc gcgtagacaa caccgagcac agctttgatc
     1741 aggcgtgggc gaaggataac tccctgacct tctgccatgt cgattagcac cgcattcagc
     1801 tggcgtttag cgtccttcga tagaaccggg gcctagtgcc aaactggctt aaacgctcca
     1861 ggttcagctc aattcgcaac cgatggctga ttatgattac tgcttattcg cttttccacc
     1921 ggccttacca gactaacttg ctcaatacct gcactatcac caccttgcag agcaacccca
     1981 caggataggt agcgccataa ccaccagcag cttcgttacg cccagtagca tcaatagctg
     2041 cgcccaggcc aggagtggaa gtcatgctgc cgcatatagc gccggacaga atgatccagt
     2101 tcatcttcaa cacataacgt ccgaacaaga agccgacaag aacggaagtc gctgtagtca
     2161 ggataccgac cagaaccaac atggcattcg gtccagtcat ggcggttgca gcctgatatc
     2221 cgtagttaag gccgacataa gccaagaagt aatacagagc tacttgtttg gtcacgttga
     2281 gggcccgctc atccatacgc agacggccac ggtcgcctag aatcaggccc gtaaccagca
     2341 ctcctcccgt ggtgcctaaa ctgaccttgc ccacggaacc caagggtata ctgatgctgc
     2401 ccagtatata tccaattagg gaaacgaatg ctatgccagg tacgcaaaac gaggcaggct
     2461 tggatgcaga ccaggtgtga ccattggcca ggtcgctatc tagttgggca acttcttgct
     2521 gcaaatccac atcgaaaagc ttcggaagca gctgcatgca tatgatgact gtaatcgcgg
     2581 caaacggata ggcgatggca taacccatgc ctacgctggc ttgcgcagca cgagctgccg
     2641 ctgatgcggg atccgggaac atgcaggcgg cagattccag agccgcacca agcccaggcg
     2701 agctggtcag cgcaccagca tacagtccgg ttatagcgtg catatccagc tttccgatca
     2761 ggcgaaaaag cgagtaggcc acgccagcac cagtagtggt tatggaaatg gctagtagca
     2821 gaaacttggc tccattaccc ctaaaaactc ttgctacatc tttggcggcg agtaatccaa
     2881 ctgaagccac aaatagaagc aggaagaaat cgaagttggc cttgtccaca aatccatttt
     2941 gcagtatgcg cactgcatag gaaggaacat caatagcccc gttcgccacc gcgatctcat
     3001 agggagccac gaaacggtcg tatatcaacc agctcaaacc catacccgca aagagtgtac
     3061 cggatgcgcc caaattgaaa cgtgctaggc tgatctttcc caagtaatga ccgcaaagga
     3121 tggccaagct caatagaaaa agtgaattcg tcagcaagtc cacgtgtcat tcatctcctg
     3181 ccttggtcac gccgtgcaaa gtgtttcact ttactgctgc ggtcggctga aagaccagat
     3241 cagcccataa aaaggatctg catgcaacat agacaactct gtctgcacaa tgccggtcac
     3301 ttgcgagggc aattcggcta cccgtacacc ggcttcttca agcgccctgc gtttatggtg
     3361 ataagtgccc ctacctcgct cgataatagc tccagcgtgt cccatacgtt taccaggggg
     3421 agcactttgg ccggcgatgt aggcaactac aggcttggtc atctgagtga tatagggtgc
     3481 cgcctcctcc tcggcactgc ctccgatctc tccaattaag acaaccactg aggtagcaga
     3541 gtcggcttca aaaagccgca gaatatcgat aaatgattgc cccacgacac gatcgccacc
     3601 aatacccact accgttgatt gaccaacgcc agaagccgtg agattgccaa ctatctcata
     3661 ggatagcgtg ccagagcgag cgaccacacc tacactcccc ggcaaaaaga tagagctagg
     3721 tgcgatgcct atcttgcatc ggccgggaga gacaatcccg tacgtgtttg ggcctgtgat
     3781 cgtggcccca atacgatctg cataagccat aatctccagg cagtcatgta ccggtacatg
     3841 ctcggttatt atcaccaatg ttctgatgcc ggcctccata gcctccagtg ctgcatcttt
     3901 aactgcagct gccggtgtaa agagcaccga agcgtcgata gaatactctt ctgtcgctgc
     3961 acgtacagta tcaaaaacag gaacgccttc tacttccatg ccacaacggc caggagtgac
     4021 tcccgccact acattagtgc cgtaagcaag catctgctgg gtgtgaaaac ggccctgatt
     4081 acccgtgatt ccctgtacca acaccgaggt ctgctcatca atcaggatag acatgctaaa
     4141 cctcctcaat atagtagaag caatgctctg taactggttg acctaacctg ctaattccac
     4201 tgctcgctga gcagcagttt gcatgagatc aaaaacctcg attccggctt ccttctcaag
     4261 aatggcacgc ccagcatcct gatttgtgcc tgctaaacga accaccatgg gaattgattt
     4321 gccgtattgt ctcctgatgc cgacaatggc ctgcgccact tcgtcgcagc gcgttatgcc
     4381 tccaaagata ttgacgagta cggccttggg ctgcttagcc aagacaagtt ccagtccttt
     4441 tcgggtagct tcttggttag ctccgccgcc gatgtccaga aaattagctg gtttgctgcc
     4501 gaaataggtt atcatatcga gcgtagccat ggtaattcca gctccattgg ccatcacacc
     4561 tatttcaccg tcaagatcca catatgatag tcccaacttg ctagcttctt gctcacctaa
     4621 agtgagctca cttaccgtcg gaatatgggc atggcgatac agcgaggcat catcgacagt
     4681 gattttggca tcggctgcat agacagtatc accgcagatc gccagcggat tgatctccac
     4741 cagttcagca tctatggcgg taaacagtcg gtaaagcgcc gtgactactt gcgttagtgc
     4801 ctttccgcag gggctgctgg gcacaatttt aagcctacgg acaatatctc gcgccaagaa
     4861 agtaggcaat ccaagctcta catctatcgg ttgtctgata atctgctctt ctggcacatt
     4921 ctcaatgtcc atgccgcctt gggcagaggc aatgagcacg ggtttcttac ttacaccatc
     4981 aactatcaag gacaaataca gttcctgccc gatggccagt ttttcctcta tcaaaagcag
     5041 ctccacaggg aagccttgca ctgtagattg aagtaactgt tcagccgcaa gttttgattc
     5101 tttggggcca tcagccagcc gaataccccc gcctttgcct cttttacccg aaagaacctg
     5161 agcttttatc attaccgggc cacccaacct ctgtgctatc tgcgcagcgg tcttgggagt
     5221 gtcgcacacc atacctcggg gcacacgtat gccaaaatcg atgaataact gttttcccat
     5281 gtattcgaat aatctcatct gatcatcccc ctcgtgtctg aataactcct actctcgtta
     5341 caagaacact acatgagctg attgagctag ctaaataaga aagtccctca cgccgtgaag
     5401 gcataaggga ccgactaacc taggccatct atcttgcatc cgatgcctgg tatatcgcac
     5461 tcctcctttc ctccgaaggc ggcgtacttt ctggaagggc aggtctcctg gcttacgctc
     5521 atcgttctgc tgacccttcc cgtctatcat gcattagaca gtggcatgcc agcgaactca
     5581 cgcttacagt ggcgggaccg ctcaggactt ccacctgatt ccctattctc cccgggaact
     5641 tcggggcacc acttccatta gatgtttagt tttcatgttt aattatatca ttaccatttg
     5701 aaactaacaa tgagttggaa actgtatatt gggcatctgt ggtattgccg cccatgaaca
     5761 aggaggacat tatattggag gagaaaaagc tacaatactg aatagagaca tttctgagga
     5821 aacacagcca taggcggcct ggaggttaca tagagccatg agagaaactg ttctgattac
     5881 cggagcgtca cggggaattg ggtatgccac tgcggagttg ttcgcggtca atggatacac
     5941 ggtctatgcc aactatcgca gcagccccaa aagcctggag cttctttccg agaagctctc
     6001 cgagcgcgga tgtaccttga tccctcttta tgcagatgtt tcgaatagca acgatgtgga
     6061 gcatatggtc tcgcagacga atggagttga tattctcatc aataatgcag ggatagcgca
     6121 gttcaagcaa ttcgtcgaca tgtccgagac tgattgggac aacatgatgt ctgtgaacct
     6181 gaaatccgtc tatctgtgca ctcactgcgt cattccacac atgataaagc gcaaacatgg
     6241 agctattgta aacatctctt cagtgtgggg tgttacgggc ggttcttgcg aggtacacta
     6301 ttccgcagca aaagccggag tgatcggctt cactaaggcc ctggccaagg agatggcccc
     6361 atccggaata cgcgtgaatt gcatcgctcc aggagtaata gaaacagaca tgaacaaaga
     6421 gctatccgac tatgacagac agttgataaa agagcaaacg ccattaggaa cgatcggcct
     6481 tcccgatcat attgcacagg cagccctctt ccttgcagaa aatcagttca ttaccggaga
     6541 aatactcaat gtcaacggag ggtttcatat ctgacatgtc gcatacgcct gaaatcgtgt
     6601 tccctctgca aactgttctg ctgggcgaaa ggtacgatgg cccttaaagc tgggttccgg
     6661 aacagatcat ccaggcccat ggaattacgg ggttgacact cgatggctgc cgctcggcaa
     6721 tccagggcca ttgacatgac agggaaccta ccgggctccg tagacctgca caatctcaag
     6781 gcacttgata acctccaccg aaccatcgca gcctctctct tcagccaatt acctttgtcc
     6841 ggcaatatca gtatgcaaac tgaaaagctc ccggcatccc tattcgattc ctggcggttc
     6901 gcggccacag acgccgcgtc cggtcagtaa cggaaccgct ccgaaccgac cataacatca
     6961 ccactgatac attcaccggc atatttccgc ctgacctcta taaggttcaa tacatcatag
     7021 ccacgctttc tgagaaaggc aataatggcg gcgttgttcg ggtgaaccca gttgtacacc
     7081 gtgtcggagc caagctcatc cgcaatgccc tctgcatatc cgtagagcct tgaggctact
     7141 ccaacccgtc tgtattccgg caagacatac aacgactctg cccaaacgac atcatcttcg
     7201 atacggcaag ctatgaagcc gataacatgg ccctcatctg actctgctac tgccaggcgg
     7261 aaacgattgg atcgatagtc tgccaattcc gtcgaagccg cgtcttggtt aggattcgat
     7321 tcttgttgct tgaacgcagc caactcgacg cgaaacgccg ctatcaaggc tactagctgc
     7381 tcgtcatctc catcaaatgc actgcgcacc accactctct atcaccaccc tacagacatt
     7441 cttgccggtc actgaccgga gttaggcttc ctcagccaga acccacagtc cgccgagcat
     7501 cccttgtctg ctgcatctac aaccattggc cggtctgaat gcgattctat gtaggcagca
     7561 ctggccccat aacaacatac tgaaacttgc ccgtcttgtc catactgctc tcgtcgactg
     7621 tggcgttttc cgatcacagg ccctgaaggg taactaaggc ttggatatcg attttccacg
     7681 tttccatctt cgttcttaac ttaagcctcc gtcactaaag gttcgagtcc gggtttatcc
     7741 ttgcgtgtga ccatgtcgag ttcctagtcc agtcactctg ctatcagttt gactctgaca
     7801 aggagagtgc taaagaccga gtacatggtg cgaactgtct atcgcaggca atagggcgta
     7861 ttcatcttga ttctagatgc tatacttatc tttccatact acgaaactat gataagtaca
     7921 atgcgtatgt tttgcaattg tgtatattgg ggtataatgt gtgtggaggt gctgacggta
     7981 atggactacc agagcatttc gaatctgaag atgcttcgct acgccaaggg tctcacccaa
     8041 caggagttgg cagacgctgc agggatttcg cgagatgcct atagaaacat tgaaaccggt
     8101 agatccgatc caaagatatc aactctgaga agcatagcaa aggcactcga cgcggagatt
     8161 gatgaccttt tcgtcccgat aaagaagcct gctcagatta gatttcgatc ctccaagcga
     8221 atgaacacca gagatatggt gctgttcgat gtagtgaaat ggatgcagga ctacaactgg
     8281 ctggagcaag cccttgacga acaaacccgg tgggcattga gcgacctatc aaccgaaatg
     8341 agcgcgcttg aaagcggaga gaagcgggct atactgtgtg cccgcaagtc ccggaaccgg
     8401 ctcgggttgg atgaggaaga accaatccgg gacatatgcg gactgctgga gtccgcaggg
     8461 gtgaaggtat atccgaaaga ggtaatgtcg gaagccttct ttggcctatc agttgggccg
     8521 gattgcggag ggcctgttgt agttgtgaac gtatgggata ggatctcagt ggagcgttgg
     8581 atattcaccg ctgcgcatga acttggccat ctcttgctcc acatggattc gtatgacgta
     8641 gacaaactcg acgagaatga ggcggaagaa caagaagcaa acatctttgc aagctacttt
     8701 ctcatgcctg aggcagtatt tcaaaaggag tggcaagata cccgagggtt gccattctta
     8761 gaccgcgtcc tcaaagtaaa gaggatgttc agagtcagct accagaccgt gctctgtaga
     8821 cttgcagaaa ccatgaatcc acgcggtaat gtgtggcgca agttccaggt tgattacaaa
     8881 aagcgctatg ggaaaaccct gaaaagagca gatgaacccc agcctgcatc aactgacaag
     8941 ttcatggctt cctactctga gtcttatcgg gctcatgaac ccgaaaggct cagcgaaacc
     9001 gacttcattc cagatagact tgctcgactg gtaaggcagg cactggaaga tgaggtgatc
     9061 tctttgagtc gcgctgcaga catgatgggc atagatattc agtcgatgcg gaacatgact
     9121 ttgtcatggg cagactgtcc atgagcatgg aacatagctc tcagtcaatc gtgatcgata
     9181 gattgacttt ctcgacagcg acaggtccat cgtgcctctg atttcaaaac accttgggaa
     9241 gctgcatgta ataaaacctg tattctaaga ggttaacggc ctccatgaat cccaatgcga
     9301 gccgttgggg atcaccatag tcgacctcac acttgatcag tataatgaag ccggttcatt
     9361 tgtgggtggc cttgcacgtg cttcgttctt gcacgccaca atggctggct gtgcattaca
     9421 aatgataagc gcttgcgaag tgagtgttgc cggcactgca ttaatgcaga ctacccccaa
     9481 ccgataccta gttccaagac ccgcgcctcc tatcatgtct cttaagccct gtaaccgact
     9541 acaaccagca cgacagactt catcgccgac acgcaggaat ggcgcttgcc agcggggaca
     9601 acatgacaat ccccgggccg cataacaacc gtagtctgtt cgtccggata gtatatctcc
     9661 atgccgccct cgaaaacgat ctgcatggaa tctgcggaat tggcgtgagg aggcacgtcc
     9721 ttaacatccc ggcgcaaagg cctctcggcc catcccatca aacagacctc tgccccatcc
     9781 acgtatccaa ggacaacgct ctgctcctca tatgggtcgc tgctggctgc aatcttcctc
     9841 tccaactctt ctctagcagc agacaagtcc atacacaaca ccccaattca ttggtggatc
     9901 gttgacttgc ataacactag cattcaaacg agcagcgtag catgaccact agcgtttcgc
     9961 catccttgtg cctggctcac tcattccagg ctcaacaggc tccgaatcac actggatagg
    10021 ctgctcacat cggaacacgg cctcaaaggg atggcgccca cgccatcggc gacgcgattg
    10081 gcgacactgc cgatgccacc gcgcccagct actgacggat tgacatctgt gggacaggcc
    10141 ggggtaggag cagaatacat tatggctgcg tcacccggat agactgcata ggcccccctg
    10201 catccggaca atgcgtcgcg ataagtatgc atcttgtgga tgtcgtctgc cttcgcactg
    10261 aggtcggatt ccgactgcct gtcgaaacgg aatttggcgt caaacaccca tattcccgcg
    10321 ggtgtgatta gggttacatc cggacggaac cagcccgagt atcccctaaa ctcccggttg
    10381 tagctgacct cgatctctcc gggaaactgg acgcgaattc ctctggcgag tctcgccccg
    10441 agccgatcgc aattcatctg tgcgcccaga ctttcgccta cacccacgcg tgcgtccatg
    10501 gctgcgatcg cgttcgcatc gaaagccacg tcagccgcgg atgcctggtt gggacgggtc
    10561 cgtacaagat cctgaacaac gttgcaaacc tgtatgaaca cccaatactc gtagagcgtg
    10621 gcagcgtctt tcagttccag gatttccttg atatcataag gccaggt
//
LOCUS       DAOSAI010000033        10545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4166_length_10545_cov_3.761773, whole genome shotgun sequence.
ACCESSION   DAOSAI010000033 DAOSAI010000000
VERSION     DAOSAI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10545
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4166_length_10545_cov_3.761773"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLM83_02305"
     CDS             <1..772
                     /locus_tag="PLM83_02305"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOK70273.1"
                     /translation="DTVLFGGELRCASCGRIADRSGMCSQCKERIAYVLPPVCSLCGT
                     PLRLLGPKTRGEPASNPENQVCSGCAMTRHYFERARAVAVYNGAARDHIHELKYRART
                     ELVAPLARMMAEVAIGDGMAGECQCVVPVPLHPEKAARRGFNQAQLLAEDVGRLLGIP
                     LEVKALQRQRSAGSQTFLDRRDRRANVIQAFLCPFPALVSQRNVLLVDDVLTSGATAD
                     GCSRALLMAGAAKVYVLTFGVSVADERDWFEAPGSSRA"
     gene            1254..4034
                     /locus_tag="PLM83_02310"
     CDS             1254..4034
                     /locus_tag="PLM83_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOK70274.1"
                     /translation="MTPDTAKPWHAVVYGEALVELRTSRQSGLSDEEVRARLEEYGPN
                     MLAEPPRRTIFQQFLAQFKDFLVILLLISAGVSGVVGEVEDALVIIAIAIVNAVIGVI
                     QEQRAENALEALKKMASPKARVIRSGEPRVIDASTLVPGDIILIEAGDSVPADARLIE
                     SGMLKVDESSLTGESEPVELCASAVLDEDAPLGDRVNMVYMGSSVSYGRGVAVVVSTG
                     MSTEMGKIAALLQEGKDEPTPLQVKLEAVGKKLGIAAVLLCVGVFAAGVFRGEAAFDM
                     FLTAVSLAVAAIPEGLPAIVTIVLSIGVQRMAAKNAIIRHLPAVETLGSADVICSDKT
                     GTLTTNQMTVRRVYVPGRVLEVSGDGYSAEGAFIESGTEIDVSEDPSITRMLASAVLC
                     NDARVRSVEIETFGSEESLDDSPVRLEMIGDPTEGAIVVAGLKAGVDKVNLTQWAPRI
                     EELPFESERKRMTTIHRLPEAYLSCTKGAPDELLKVCSSVYDGTEIIPLTEALREEIR
                     RVNAEMASSALRVLAVAQREFDELPPKISVDTIENGLVFLGLIGMQDPPRAEAKTAVQ
                     ECFDAGITPVMITGDHKVTAVAVARDVGIMTPDTIAITGAELSQMSNEELDDAVDRVR
                     VYARVAPEHKVRIVDAWKRKGHIVAMTGDGVNDAPALKRADIGVAMGITGTDVAKSAA
                     DMIITDDNFATIVTAVREGRVVFDNIKKSVQYLVSCNVGEIIVIFAAIMLGLPRPLLP
                     IQILWLNLVTDGLPALALGVDPPAPDLMRRKPRRGNDDVLGHAAVSRLVVYGISVALL
                     GLSSFAMGLGWPGPLDTSAASLAEARTMCLLTMSFCQLVHAFNFRSELDSLFKVGMFT
                     NRRLVMAFVASAILQLAVVYMAPIRGFFDLVDVHGEKLVNSLALALIMLVVGEVTKFV
                     SREVSWRRGHEA"
     gene            4176..4721
                     /gene="raiA"
                     /locus_tag="PLM83_02315"
     CDS             4176..4721
                     /gene="raiA"
                     /locus_tag="PLM83_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOK70275.1"
                     /translation="MEITVVGRNIAVTDALRAYAEKKVDKLQKYFEREIVDAQVTMAV
                     ERGIHTADVTIQVDGLLLRGEERTGDMYASIDGAVDKIERQIRKHKTRINRKLRQDGN
                     RLVESAVGHGAAADASEPSELKVVRVKRFAMKPMSVEEAIMQMELLGHDFFVFMDDST
                     EDVNVVYKRKDGNYGLIEPEF"
     gene            4814..5950
                     /locus_tag="PLM83_02320"
     CDS             4814..5950
                     /locus_tag="PLM83_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70276.1"
                     /translation="MDGLVALVFEGGRRQGEISGWVERVREELAISLCRKLASSGVFD
                     DVVLITDGPALADRAGAIGGVSVELDRCCDNYHFGARLAQALQARAAMGFVYMGGGSG
                     FFMTVPEIVNFAEMVKNNPEAIVANNLFSADMFGTASSDAIARLNLPSNDNSVPMIAG
                     AAGMDLVALPPSVGSVFDVDTPSDLLVLASVIPQLAPEEAAVLDAIRSGPIEPAMSRL
                     AEAREVLGRDFAEVGLFGRVSPATTMDLNTCTRCRLRVFSEERNMRALGRDVPGGARS
                     LIGKHIEAVGPEAFFADLAWCCDAAFIDTRVLFFHMRADLSQEERFASDLFLYDRLAD
                     NGVARFVKAAAEAGLPVTLGGHSLVSGGVRLLAAAEHGGRCDVV"
     gene            5989..8550
                     /gene="secA"
                     /locus_tag="PLM83_02325"
     CDS             5989..8550
                     /gene="secA"
                     /locus_tag="PLM83_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOK70277.1"
                     /translation="MLNLFKRIFDVNAKELRDLGARVDAVSALAPEAQRATDEQLRAR
                     TGEFKGRIERGEPLDSILPEAFATVREVARRTIGLYPYDVQVMGGIVLHEGKIAEMKT
                     GEGKTLVATMPTYLNALEDRGVHIVTVNDYLARRDAEWMGPIYNALGLTVGVIVHDQQ
                     GTARKDAYMADITYGTNNEFGFDYLRDNMVTSMDGRVQRGHNYAIVDEVDSILIDEAR
                     TPLIISGQAEESTEKYFVFAKIAQSLKEEEDYTVDEKAKTVSITESGVARVERWLGVS
                     NLYDEENIELTHHLNAALRAKALMRRDRDYIVKDNEVIIVDEFTGRLMFGRRYSDGLH
                     QAIEAKENVHVQRESQTLATITFQNYFRMYQKLAGMTGTAVTEESEFIKIYNLPVVQI
                     PTAKPMIREDLPDAVYKTERAKFQAVVDEICERHATGQPLLVGTVSIEKSELLSDMLS
                     KRGVPHQVLNAKNHEKEAEIIKLAGQPGAVTIATNMAGRGTDIVLGPGVPELGGLHVI
                     GTERHESRRIDNQLRGRSGRQGDPGSSRFYVSLEDDLMRLFGGDYISGMMERFGWTED
                     EAIEHPTLTKAIERAQQKVEGRNFEIRRQVLEYDDVMNKQREAIYARRAAILEGTDLR
                     DQAIELVGTMVEGLVRAYADEKLGHGEWNLRGLSMEIASILNVRSGPDLEAREFHSWT
                     ELHEAVLTIFMRAYEQKEAAVGAERMRELERAIMLYLIDSKWVDHLQAMDMLRDGIGL
                     RAYGQLDPLVEYKRESYMMFNDLLRSIGEDFVRYVFRVRIRIEGESESASPQDTGAAA
                     AAAGLRRPKYAVSTNRGTDSSTKAPARRDGKKIGRNDPCPCGSGKKYKKCCGAQK"
     gene            8573..9695
                     /gene="prfB"
                     /locus_tag="PLM83_02330"
     CDS             join(8573..8629,8631..9695)
                     /gene="prfB"
                     /locus_tag="PLM83_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076737.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOK70278.1"
                     /translation="MRAELEQPIELLSERIDEMGPIFDIPGKLERLAQCEARAAEPGV
                     WEDRDSAQSLMQKISRLQAEIASYDSIVSRFEDLKILAELADEEDDEDAIGECFEEIE
                     ALESAVDKVEISHLLSGPHDSANAIMSIHPGAGGTESQDWAEMLMRMYARWAERNGYT
                     VEVADLLPGEEAGIKSATLIMHGHNAYGYLKNEKGVHRLVRISPFDASARRHTSFASV
                     DVVPEMDEEDVEIDMADLKIDTYRSGGAGGQHVNKTDSAVRITHLPTGIVVQCQNERS
                     QHSNKETAMRVLRARLAELQREEKEKELSAMRGEQHEIAWGSQIRSYVFQPYTMVKDH
                     RTELETGNVDAVMDGEIDEFIEAQLRLSAASANRDAGSE"
     gene            9697..10422
                     /locus_tag="PLM83_02335"
     CDS             9697..10422
                     /locus_tag="PLM83_02335"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HOK70279.1"
                     /translation="MARRVSPRRIPRIAIIMLLVVAATEVAVPFYVEKKVESAVRSGF
                     ASAESISVAARAMPALKLLYGHFDRLSIDIEEAVSSGFVIRRISLDSSDARIPLGRFE
                     PGSSRWARALGNSQLEVIIDESNVNDYLGHRDDYLRAFGVRFHPGIAELAGRLFLGGF
                     SVGVESQGHFSIGDGVKLVYVVDKLIVEKSEVPAFIVDMFEDGIAIGIDLSDLPFDIE
                     LESVEVGEGMVRLLGRTSEGGSL"
BASE COUNT         2468 a         2405 c         3266 g         2406 t
ORIGIN      
        1 ggacacagtg ctgttcggag gggagcttcg ttgcgcgtct tgtgggcgga tagctgatag
       61 aagtggaatg tgcagccagt gcaaggaaag gatcgcttat gtccttcccc ccgtctgttc
      121 gctatgtggt actcccctca ggctgctggg gccaaaaaca cgaggcgaac ctgcttcaaa
      181 cccggagaat caggtatgtt cgggatgtgc gatgactcgg cactacttcg agcgcgcaag
      241 ggctgttgca gtatataatg gcgcggcgcg ggatcacatt catgagctga aatacagggc
      301 gcgaacggaa ctcgttgcgc cgcttgcccg aatgatggcg gaggtggcca tcggcgacgg
      361 gatggccggg gaatgccagt gtgtagtccc ggttccgctt caccctgaga aggcggctag
      421 gcgcggtttc aaccaggctc agctgcttgc agaagatgtg ggacggctct tggggattcc
      481 gttggaggtc aaagccctcc aacgtcagcg aagcgcgggc agccagacat ttttggatag
      541 gcgtgatcgc cgagcaaacg tgatacaggc tttcttgtgc ccttttccag ctctcgtttc
      601 ccaacgcaat gtgcttctcg tagatgacgt tctgacttct ggcgccactg ctgacgggtg
      661 ttccagggct ctcctgatgg cgggggcggc gaaagtatac gttctgacat ttggcgtgtc
      721 agtggctgat gagagggatt ggtttgaggc tccgggaagc agccgagcat gagggttaca
      781 ggccttactt tcgagtctct ctttgagggg cacggagatt aggtttagca gttcatcatg
      841 tcgaagcgca actcagatcg gaaaatgaac tgtgggcggt attctctttc cagtatcaga
      901 tggctcctgt caatgcggcc ttttgcgatc ggcccgagcc tggagatcat agctggtcta
      961 tcagcggcta gcggtggccc agtactggtt ttggttaaga cattggttgc gcacaggact
     1021 tctcaggata tataattaac tccaaatcca gccgtgagcg cgcttggggc gcgcagattg
     1081 cttttgaacg caggcatcat catggtattg gcaatttcgg catgagggtt tgttggtcca
     1141 ttcaggatga acgagcccgt caactccgtt gccatcagtt tttctcaaga gacggttggc
     1201 gcggtggcgg gcataatgtt atctatagac atggttatat ggaggtatga ttgttgacac
     1261 cggatacggc aaaaccgtgg cacgcagttg tttatgggga agctctcgta gaactaagaa
     1321 cctcgcggca aagtgggctt tcggatgagg aggttagggc caggcttgaa gagtatggcc
     1381 caaacatgtt ggcagagccg ccgcgacgga cgattttcca acagttcttg gcgcagttca
     1441 aagatttctt ggtgattctt ctcctaatat cggctggtgt gtccggagtt gtgggcgaag
     1501 tcgaggatgc tttagttatc attgcaatag ccatagtaaa cgccgtcatc ggggtaattc
     1561 aggaacaacg agcggagaat gcgcttgaag cgctgaaaaa gatggcatca cctaaggcca
     1621 gagtcattag gtccggtgag ccgagggtta ttgacgcgtc gactctggtt ccgggcgaca
     1681 taatccttat cgaggctggt gacagcgttc ctgcagacgc gaggctcatc gagtccggca
     1741 tgttgaaggt ggatgagtcg tcgcttacgg gggagtcgga acctgttgag ctttgtgcat
     1801 cggcggtgtt ggacgaagat gctccgctag gcgacagggt gaacatggtt tacatgggca
     1861 gttctgtgtc gtacggaaga ggcgttgcag ttgtagtgtc cactggaatg tctactgaga
     1921 tggggaagat cgctgctctt ctgcaggaag gcaaggacga gcccacccct ctccaagtca
     1981 aacttgaagc ggttgggaag aagctgggaa tcgcagctgt tctgttgtgc gttggggttt
     2041 ttgccgccgg tgttttccga ggagaggccg ctttcgacat gtttctgaca gcagtgagcc
     2101 tggcagttgc tgcgatacct gagggccttc ctgctatagt aacaatagtg ctttccatag
     2161 gagttcaaag aatggcggcc aagaacgcaa ttataagaca tcttcctgca gtagagacat
     2221 tgggaagcgc cgatgtaatc tgctctgaca agacggggac attgaccacc aatcagatga
     2281 cggttaggag ggtctacgtg cccggcaggg ttctggaggt cagcggggac ggatattccg
     2341 ctgagggagc attcatagaa tcaggcacag agatagatgt ttctgaggat ccctcaatca
     2401 cgcggatgct tgcgagcgcg gtgttgtgca atgatgctag ggtgagatct gtcgagatag
     2461 aaacttttgg gagtgaggaa agtcttgatg actcgcccgt taggctggag atgatcggcg
     2521 atcccacaga gggggcgata gtcgttgcgg gcctcaaagc cggagtcgac aaagttaacc
     2581 tgacccagtg ggcgcctagg atagaggagc tgccatttga atctgaaagg aaacggatga
     2641 ccaccattca tcgacttccc gaagcctatc tcagttgtac taagggtgcg cctgatgaac
     2701 tacttaaagt ttgctcttca gtatatgatg ggacggagat aatcccgctt actgaggctt
     2761 tgcgcgaaga gatccgtaga gtcaacgccg agatggcctc atctgccctt cgggtgttag
     2821 cggtggctca aagagaattc gacgagctgc ccccaaagat cagcgtagac actatcgaga
     2881 atggcctcgt ttttcttggc ttaattggca tgcaggatcc gcctcgggct gaagcaaaaa
     2941 ccgctgttca agaatgcttt gatgccggga taacgccggt catgatcacc ggggatcata
     3001 aggtgacggc agttgcggtg gcaagagacg tggggatcat gacgcctgac acaatcgcta
     3061 tcaccggcgc cgagctttcg cagatgtcta atgaggaatt agacgatgcc gtagaccgag
     3121 tcagggttta cgccagggtt gcgcctgaac acaaggttcg cattgttgac gcatggaaac
     3181 gcaagggaca tatcgtagcg atgaccggtg acggagtcaa tgacgctcca gccttgaaac
     3241 gtgccgatat tggcgtggct atgggtatta caggcacaga tgtcgcaaag agcgcggctg
     3301 acatgataat cacagacgac aacttcgcca cgatagtgac tgcggttcgc gagggtcggg
     3361 tggtctttga taacataaaa aagtcggtgc agtatctggt atcgtgcaac gtgggagaga
     3421 ttatagtaat atttgcagct atcatgctcg ggcttcccag gccactgttg cctattcaga
     3481 tactctggct caacttggtg acagacggtc tgccagcgct ggctcttgga gttgatcctc
     3541 cggctcctga tttgatgagg agaaagcctc gcagaggaaa cgatgatgtt ttggggcatg
     3601 cggccgtcag caggctagtt gtgtatggga tatcagtagc gttgctgggc ctgtcgtcgt
     3661 ttgctatggg gcttggatgg cccggaccgc tcgatacgtc tgcggcaagt ctcgcggagg
     3721 ctcggactat gtgtcttctt actatgagtt tctgccagct agttcacgcc ttcaatttca
     3781 gatcggagct tgattcgttg ttcaaagtgg ggatgtttac caacaggcgg ctagtcatgg
     3841 cttttgttgc atcagccata ctacagttgg cagtcgtgta catggcgccc atccgcggct
     3901 tctttgatct tgtggatgtg cacggggaaa agctcgttaa ctctctggca ttggctttga
     3961 tcatgctagt agttggggag gttaccaaat tcgtttcccg agaagtgagc tggcgacgcg
     4021 gtcacgaggc gtgagagctt cgagtgctgt cacggctgaa cgtctgcatt ggaaggacac
     4081 gcatttctcc gactgccgca ggatttttcc cacaaagggt gaagtactga agcagccgat
     4141 gtgcgccaca actagttcgg aaggggctgg tatgaatgga gatcacagtt gtaggtcgca
     4201 atattgctgt cacagatgct ctacgggcct atgccgagaa gaaggttgat aagctccaga
     4261 agtacttcga gcgcgagatt gtggatgctc aggttaccat ggcagttgaa cgaggcattc
     4321 acacagcgga cgtaacgatt caagtcgatg gtctgctgct ccgtggtgaa gaacgaaccg
     4381 gtgacatgta tgcatcgatt gatggcgctg tggacaagat tgaaaggcag ataagaaagc
     4441 acaagactag aataaatagg aagctaaggc aggatggcaa taggctggtg gaatccgctg
     4501 tcggtcatgg ggctgctgcg gatgcgtctg aaccctccga gctcaaagta gtcagggtta
     4561 agagatttgc gatgaaaccc atgtcggttg aagaggccat tatgcagatg gaactcttgg
     4621 ggcacgactt tttcgttttc atggatgact ccactgaaga tgtgaatgtg gtctacaagc
     4681 gcaaagacgg gaactacggt ttgatagaac ccgaattcta aaccgcgcgg ggatcctgcg
     4741 tagcggggcg gtgcatattg catcgccccc gatatatttg gaagtcattt ggcgtgtgac
     4801 gggggtggtc atcgtggacg gactggtggc actggttttc gaaggcggga ggcggcaggg
     4861 cgaaatcagc ggatgggttg agcgggtcag agaggagctg gcgatatctc tctgcagaaa
     4921 attggcgtcg tcgggggtat tcgatgatgt cgtgttgata actgacggac ctgcgcttgc
     4981 cgaccgagct ggtgctatcg gcggagtatc tgttgagctc gaccgttgct gtgacaacta
     5041 ccatttcgga gcaaggcttg cgcaggcgtt gcaggcccgg gccgccatgg gatttgtcta
     5101 catgggtgga ggttcgggct tcttcatgac tgtcccggaa attgtgaact ttgcggaaat
     5161 ggtcaagaat aaccctgagg ccattgtggc caacaacctc ttctcagccg acatgttcgg
     5221 aaccgctagt tcagatgcta ttgctcgcct aaatcttccg tcgaatgaca attctgtgcc
     5281 tatgattgcc ggtgccgccg gaatggatct ggtggcgttg ccgccttcag tcggatcggt
     5341 tttcgacgtg gacacgccct cggatttgct ggtgttagcg tcggtcatcc ctcagcttgc
     5401 ccccgaagag gcggctgtac tcgatgcaat acgctcaggc ccgatagagc ctgccatgag
     5461 tcgtcttgcc gaggccaggg aggtgttagg cagagatttt gccgaggttg gcctcttcgg
     5521 acgagtctcc ccggctacga caatggacct gaatacttgc acccgttgca ggctgagagt
     5581 gttttctgag gagcgcaaca tgagagctct tggccgggac gtgcctgggg gcgcgcggag
     5641 cctcatcggc aagcacatag aagcagttgg accggaggct ttcttcgctg atcttgcatg
     5701 gtgctgtgac gcagcattca tcgatacgcg agttcttttc ttccacatgc gggcggactt
     5761 gtcgcaggag gagagattcg cgtccgatct tttcctctat gataggttgg cggataatgg
     5821 cgttgcgcgg ttcgtgaagg cggctgctga ggccgggctg ccggtgacac ttggaggcca
     5881 ctcccttgtc tctggcggag tcagactgct tgctgcagca gaacatggcg ggagatgtga
     5941 tgtggtataa ttgcttagtc ggcaaagaca caagcgaggt ggccatgggt gttaaacctt
     6001 ttcaagcgga tatttgatgt gaatgccaag gaactgaggg atctgggcgc cagggtagat
     6061 gcggtatcgg ctctcgcccc cgaggcccag cgggccacag atgaacagct gcgcgcccgc
     6121 accggagagt tcaaaggcag aatagagcga ggggagccac tggactcgat tcttccagag
     6181 gcttttgcaa cagtccgcga ggtcgcccgg cgaaccatag gactttaccc atacgatgtg
     6241 caagtgatgg gcggcatagt gctgcacgag gggaaaattg ccgagatgaa gactggcgaa
     6301 ggcaagaccc tagtggccac catgccaact tacctcaatg cccttgaaga ccggggtgtg
     6361 cacatcgtca ctgtcaacga ttatctggcc cgtcgcgacg ctgaatggat gggccccata
     6421 tacaatgcct taggcctcac cgtaggagtg attgtccatg accaacaggg aactgcgcgc
     6481 aaggacgcct acatggcaga tatcacctac gggacgaaca acgagttcgg attcgattat
     6541 cttcgtgaca atatggttac ttcgatggat gggcgtgtcc agcgcggaca taactacgcg
     6601 atagtcgacg aagtcgactc gatcctcatt gatgaggctc gaaccccttt gatcatctct
     6661 ggacaggcag aagagtccac ggagaagtac ttcgtctttg caaagattgc tcaaagcctc
     6721 aaagaggaag aagactacac ggtggacgag aaagccaaaa cggtgtcgat cactgaatca
     6781 ggggttgcgc gggtggaaag atggctaggc gtgagcaacc tgtatgacga ggagaacata
     6841 gagctgacgc accatctgaa tgcagcttta agagctaagg ctttgatgcg gcgggataga
     6901 gattacattg tcaaggacaa cgaagtcatc atcgtagatg aattcacggg aaggctcatg
     6961 tttgggaggc gttacagcga tggactgcac caggcgatag aggctaagga gaacgttcat
     7021 gtccagcgcg agagccagac attggcgaca ataaccttcc agaactactt ccgcatgtac
     7081 caaaagttgg caggtatgac aggcactgca gtcactgagg agagtgagtt cataaagata
     7141 tataatctgc cggtggtgca gatacctact gcgaagccca tgatcaggga ggacctgcct
     7201 gatgcggtct acaagactga gcgagcgaag ttccaggccg tggtcgatga gatttgcgag
     7261 aggcatgcca ctgggcagcc gcttcttgta gggaccgtct caatcgagaa atccgaattg
     7321 ctttcggata tgctttccaa aagaggcgtc ccccatcagg tgctcaacgc gaagaatcac
     7381 gaaaaggaag ccgagatcat aaaacttgcc gggcaacctg gcgcagttac tattgcaaca
     7441 aatatggcgg gacgcggaac ggatattgta ctgggaccgg gcgtcccaga gcttggcgga
     7501 cttcatgtaa tcggcactga gcggcatgag agccgtagaa tcgataatca gctgcgcggg
     7561 agatcaggga ggcaaggcga tcccggctct tccaggttct atgtgtctct cgaggacgac
     7621 ttgatgcgcc tcttcggagg agactacatc tccggaatga tggagcgttt cggctggacc
     7681 gaggatgagg cgatcgaaca tcccaccctg acaaaggcga tcgagcgcgc tcaacagaag
     7741 gtggagggac gcaacttcga gatcaggcgg caagttctcg aatacgacga tgtcatgaac
     7801 aagcagcgcg aggcgatata tgccaggagg gcggcgatcc tcgaaggaac cgacctgagg
     7861 gaccaagcca tagagctcgt aggcacgatg gtggaaggtt tggttagagc gtatgccgat
     7921 gagaagcttg gacacggcga atggaacttg cgagggcttt ccatggaaat agccagcata
     7981 ctcaatgtga gatccgggcc cgatcttgaa gcgcgggagt tccattcctg gacagagctt
     8041 cacgaagccg tgctcactat attcatgcgg gcatacgagc aaaaggaggc tgcagtaggc
     8101 gccgaacgca tgcgtgagct ggaacgtgct atcatgctct acttgataga ttccaaatgg
     8161 gtggatcatt tacaggctat ggacatgctc cgagatggaa tagggcttag ggcctatggt
     8221 cagctggatc cccttgttga gtataagcgg gaatcataca tgatgttcaa cgatctactg
     8281 aggagcattg gtgaagattt tgtgaggtac gtattccgag tccgaatccg cattgagggc
     8341 gagtcggaat cggcgtcgcc tcaggatacg ggtgcggctg ccgcagccgc tggcctccgt
     8401 cgcccgaagt atgctgtatc taccaacaga ggcactgatt catcaaccaa agcgccggcg
     8461 agaagagatg gcaagaagat cggaagaaac gatccctgcc catgcggcag cggcaagaaa
     8521 tacaagaagt gctgtggcgc ccagaagtaa atcagataag ggtgatgagt atatgcgagc
     8581 tgagcttgaa caacccatag agctgcttag cgagagaatc gatgaaatga ggacctatct
     8641 ttgacatacc tggtaagttg gagcgccttg ctcaatgcga ggctcgagct gctgagccgg
     8701 gggtgtggga agatcgtgat tcagcgcagt cgctgatgca gaagatatcg agattgcagg
     8761 cggaaattgc ctcttatgat tcgatcgttt caagatttga agacctgaag atactggctg
     8821 aacttgcaga tgaagaagat gacgaggacg cgatagggga gtgctttgag gagatcgaag
     8881 ctcttgagtc agcagtggac aaggtggaga tttcccatct gctttcaggg cctcatgatt
     8941 ctgcaaatgc catcatgtcg atacatccgg gcgctggggg cactgagagc caggactggg
     9001 ccgaaatgct tatgcgaatg tatgcaaggt gggctgaacg caatggctat accgttgagg
     9061 tagccgatct gctgcccggc gaggaggccg gcataaagag tgcaacattg atcatgcatg
     9121 gacacaacgc ctatggatac ctgaagaatg aaaaaggagt ccacaggctg gtgcggatat
     9181 ctccgttcga tgcttctgca cgtaggcata catcctttgc ctcagtcgac gtagtgcctg
     9241 aaatggatga agaggacgta gagatcgaca tggcagacct caagatagat acgtatcgtt
     9301 caggaggagc tggtgggcag cacgtcaaca agacagacag tgctgtgagg attacgcacc
     9361 ttcccacagg cattgttgtt cagtgccaga acgaacggtc gcagcacagc aacaaggaga
     9421 ctgcaatgag ggtgctcagg gctcgactgg cggagcttca gagggaggag aaggagaagg
     9481 aattgtccgc catgcgtggc gaacagcacg agatagcctg gggaagccag atcaggtcat
     9541 atgttttcca gccctatacc atggtcaagg atcacaggac agagttggaa actggaaatg
     9601 tggacgccgt tatggatgga gaaatcgacg agttcatcga ggcgcaactg agattgagcg
     9661 ctgcctctgc taaccgagac gcagggagtg agtagcatgg ctcgtcgcgt cagcccccgg
     9721 agaatcccgc ggatcgccat catcatgctt cttgtggtgg ccgccacaga ggtggcagtg
     9781 cctttctatg tcgaaaaaaa ggtggaatcc gcagtaagaa gcggttttgc ttctgccgaa
     9841 tcgatttctg tggcggcacg agcgatgcct gctctgaaac tcttatatgg acactttgac
     9901 aggctttcaa ttgatattga agaagctgtg tcctcgggct ttgtgatcag gcgaatttcg
     9961 cttgactcgt ccgatgctcg aatccctctg ggaagattcg aaccgggatc tagccgctgg
    10021 gctcgtgctc tcggcaattc ccagctggag gtcattatag atgagagcaa tgtcaatgac
    10081 tacttaggtc acagggacga ttaccttcgc gccttcggtg ttcggttcca ccccggaatc
    10141 gcagaattgg cgggacggct tttccttggc ggcttcagtg tgggcgttga atcgcaggga
    10201 cattttagca taggagacgg agtcaaacta gtctacgtag ttgacaagct cattgtggag
    10261 aagagtgaag ttccagcttt tattgtggac atgttcgaag atgggatagc tattgggatc
    10321 gacctttcgg atctcccctt tgacatagag ttagaaagcg ttgaagtggg cgaaggcatg
    10381 gtccgtctgc tgggtcgcac ttcggaggga ggcagtctgt gatgggccac aggaaacctc
    10441 ggtctattgg gtggctgatt gcgctgatcg tgttggcttc agtatcatct gcttggatag
    10501 cctggcaaag aaatcgtgtt gagaaggcta acagtgttgt tgagc
//
LOCUS       DAOSAI010000034        10532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4170_length_10532_cov_3.847953, whole genome shotgun sequence.
ACCESSION   DAOSAI010000034 DAOSAI010000000
VERSION     DAOSAI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4170_length_10532_cov_3.847953"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..383
                     /gene="flgM"
                     /locus_tag="PLM83_02340"
     CDS             60..383
                     /gene="flgM"
                     /locus_tag="PLM83_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOK70280.1"
                     /translation="MKISGSRGFDRINLYSQLAKKAERAERTGEVKPDEVEISDRGKE
                     IHHAMQALSSVSDVRDDKVEAVRSRIESGTYEVSGKDVARKLFAGAVRRGDLGGLRKG
                     NGEDS"
     gene            349..849
                     /locus_tag="PLM83_02345"
     CDS             349..849
                     /locus_tag="PLM83_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOK70281.1"
                     /translation="MDYAKEMARILNREFALCSELLYVARAQRSVITSGDVAALAELV
                     SRAEEIIGAIRDEEIGLADLAKRWADETEVEVEDAEQALAVMIASFKEEEMAEFEVLR
                     DSIAGILAEIDSANAINAMLIQDAIEYIENTVRLIAEADGTNSVYSRLGRMDKKAVSS
                     AVDDTA"
     gene            875..2203
                     /gene="flgK"
                     /locus_tag="PLM83_02350"
     CDS             875..2203
                     /gene="flgK"
                     /locus_tag="PLM83_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOK70282.1"
                     /translation="MYSTFVGLETARRALYAQRAAIDITGHNVSNVNTPGYRRQRPVI
                     EATPPAPGALQPGTGVKVAGIERMRDQFVDTQIRHTLASLGRWEARQEILEEIQEVFK
                     EPSDIGISAMMDRFWISWQELSGNATDPGRRTSVQQEAISLASAFNLAAAQLNETKAD
                     IDQNIKSLVHRVNTTASSIAELNREIVRGNVVGENVADLLDRRDYLVQQLASDVDVAV
                     VEDNSGLVHVTVGGRILVAGTDARELAEDALITGGRIKGLNEAANMIIPGYLKELDDL
                     ANALIEKINEQHMAGYDLFGNSGDVFFEGEGAAGIRLSEEIEANPGLIAAADQTDSPG
                     NGRNALAMAQLKDGLTMVGGTATFRDFYGSILTGLGLETQESMRSREVYEGVLEQQKA
                     RQESVSGVSLDEEMIMLIKYSSAFDAAARLVTAVDEMLDRLINGTGLVGR"
     gene            2280..3188
                     /gene="flgL"
                     /locus_tag="PLM83_02355"
     CDS             2280..3188
                     /gene="flgL"
                     /locus_tag="PLM83_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOK70283.1"
                     /translation="MRVTNNVMMERAMRNVNVAFERYVKRQEQLASGCSINRLSDDPH
                     GAAKAVRLRSTLKGLEQYKTNADIADTKLRAYDGVLDKVGSIMNRLRDLVGRAASDTV
                     TPTERDGIAVEVRELFEEVVNAGNEMHNGEHMFAGHKVGQAPFKVERDADGRVTGVLY
                     HGDSGKDAIEIGPGIVISGSLAGDEVFMADGANVFDAMIDIERHLRENDVESLSEEDL
                     GRVDAVVDNILKHRAEIGGKGKRLELVMDRLESDSISIEGLLSKTEDIDFAEAIMRLC
                     EQEIVYYAALSAASKSVQAGLLQFLR"
     gene            3202..3684
                     /locus_tag="PLM83_02360"
     CDS             3202..3684
                     /locus_tag="PLM83_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOK70284.1"
                     /translation="MEHGFDAEEIQLVVFELADESYGVEISRVQDINRMQEITEIPHA
                     PESVVGVINLRGRVIPVVDLRTRFGLPPAEHTKSTRIVVVQMGEEPIGMIVDAVSQVL
                     RIPTKIIEPPSPVLSSVDSRYLRGIAKLEDELVILLDLDYVLSKDEQEFISEAMAESV
                     "
     gene            3696..5867
                     /locus_tag="PLM83_02365"
     CDS             3696..5867
                     /locus_tag="PLM83_02365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOK70285.1"
                     /translation="MLEFPGMTADDIKVFLIEAEEQLQSLEDGLLELEQAGEDDEVIG
                     QIFRAAHTLKGSSATLGHDKMANLTHSMESVLDLVRKSQLEVSPDLMDVLFECLDVLR
                     VLNREVETGQESGEDITVLTGRLLDIIAFAEEGGSGCISGESEAGGSGAQSTVAAVDG
                     GIMHGPGDVELCIAFADTCQMPSVRAYQVVDRLSVMGNIIYTRPSMELIEAGQDIGEL
                     TVAIEWDGDSPQPLVEAAMDVSDVESALVVQVRPAGSLKGEADAAAEASRATVASGPK
                     ESEPTGVEAGRKAGVNRGSRSATSTRATTVRVDVARLDALSNLVAELVIDRTHLAQLE
                     SKLAEKHGGDELVAELNRTSTHIGRLTTELQEAINKARMFPIDNVFRRFPRMVRDLAQ
                     SQGKEIDFIIEGEDTELDRSVSEEIGDPLIHLIRNAVGHGIETPEERVAAGKAPKGTV
                     RLSAFHQENYIVIEIADDGRGIDPEAVKESAVRKGLISEEAAARMSDRDAVNLIFSPG
                     LSTSKKVDDVSGRGVGMDIVRKNIERLNGSISINTRLGEGSTFTVKLPLTLAIIRALL
                     VAYDSRIYAIPLASVMEITRIEPTDIHTVHKREAIRLRGDVIPLLWLGEIFSKRGEER
                     GAAEWMAADGRPEDGAAGEAQSRRMFAVITSSHEGQVGLVVDSLVGEMEIVIKSLGSF
                     IGNIPGVSGVTILGDGRVALILDIPSLVRRVVEERNGAERG"
     gene            5848..6357
                     /locus_tag="PLM83_02370"
     CDS             5848..6357
                     /locus_tag="PLM83_02370"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOK70286.1"
                     /translation="MALNGAKDVVPIGIGEMHVSDKPGDVLVAYGLGSCVGVVIHDPI
                     GKVGGMVHVALPESLSAPRSEERPDARYADVGVPMLVGEVLRLGGMKARLVARIAGGA
                     KMFDVTPELEFMDIGARNSVAVRAELEAMGIPIVAEDIGGADGRTLRYVVGREQVFVR
                     KLGMREVEL"
     gene            6380..6736
                     /locus_tag="PLM83_02375"
     CDS             6380..6736
                     /locus_tag="PLM83_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK70287.1"
                     /translation="MPTVLVVDDAAFMRMRAGKLLSQSGYEVIEAENGLDALNKYTEY
                     EPDLVLMDITMPVMDGLTAVREIRQVDPEAKIIMVSAMGQQAMVIEAIKLGAKDFIVK
                     PYEPDNVLASVKKLIG"
     gene            6736..7803
                     /locus_tag="PLM83_02380"
     CDS             6736..7803
                     /locus_tag="PLM83_02380"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOK70288.1"
                     /translation="MMPISVLVVDDSAFMRKVVSEMIASDPELSVAGTARDGKDAIEK
                     ARSVRPDVITLDVEMPVMDGLEALPVLAGELGIPVVMLSSLTQEGAEVTLRALGLGAI
                     DFVPKPSGAISLDIDVVREDLLAKVKAAAKVSSARVRGLARDARVPLTGLGREVKEAK
                     SGARAARARNVVVIGTSTGGPRALTQVVPWLPPNLPAGVLIVQHMPAGFTKSLADRLN
                     QTSKIRVLEAQGGEAITEGAALIAQGGLHMVVEPDGTVRLDSSPPMHGVRPAVDLTMN
                     SAVDLFGKKTVGVIMTGMGSDGADAMARVKRMGGKTVCEHESTCVVYGMPRAVVEQGD
                     ADRVVPLPKIADEIVDAVLSL"
     gene            7845..8657
                     /locus_tag="PLM83_02385"
     CDS             7845..8657
                     /locus_tag="PLM83_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOK70289.1"
                     /translation="MSLVDNADYEVFRRKVLALTGIDLGLYKSQQMQRRIGSLMERAG
                     VSTFAAYGDLLGRDRAELDRFCDYITINVSEFFRNPEKFDELEREILPELLARSRNLS
                     IWSAGCSNGAEIYSVAMILQEIAPRGYHRLLGTDIDTGILSKALAGVYGPQDVRNVSA
                     ARMAKYFKADGESYVLLPEIRSKVQFKMHNLLSDPFERGFDLIICRNVVIYFTDEAKQ
                     DLYGKFWESLKDDGVLFIGGTETILNAKDIGFAPMASFFYKKVPVRKEVGVG"
     gene            8654..9694
                     /locus_tag="PLM83_02390"
     CDS             8654..9694
                     /locus_tag="PLM83_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOK70290.1"
                     /translation="MRRIAVEHMKPGMTLAEGIYNGSGVTQVSPGSVLSETAIARIRQ
                     LGISHVFIEDPRTDDLVIRDAVPWNVAARGRELLRDLAERVREAGGPDHVKVPFDDLL
                     ELVHDIEDGLQGTDLNVLEIPLVTRPEDYRYAYPINVAALSMFVTAKAGLPRRTTDVG
                     LAALLLDIGMELAPEEPEKHPEMSLAVLRQDERFSAYSRAIVFQHEERHDGSGYPRGI
                     AGDDIDPLAKIVSVADMYCSLISEGNPMGERLAPQEAFDYVTSAAGFEFERTTVLGVM
                     QYVALYPVGTMVRLNTGECGVVTRVYKGLTTRPTVRVLYGADGVEVARPYEMVLAEPE
                     HQTVLVAGVCED"
     gene            9716..10183
                     /locus_tag="PLM83_02395"
     CDS             9716..10183
                     /locus_tag="PLM83_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOK70291.1"
                     /translation="MKVQSSRFGTLTVDGDKIMSFPKGIPGFEDSRRFFLLDHPGSAK
                     VKWLHSADDPGLALVVASPFELFASYEPEVPDGVAEALGAEGPEDMLVLTVLSVHAGN
                     PPKVTANLLAPIVVCSATMRGMQVVMEAGKYGVRHEMELVPEKCEAGAKSSVG"
     gene            10302..>10532
                     /locus_tag="PLM83_02400"
     CDS             10302..>10532
                     /locus_tag="PLM83_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOK70292.1"
                     /translation="MRINHNLSALNAWKNMSVNDSGQNKSLEKLSSGLRIGRAADDAA
                     GLSISEKMRGQISGLNQASRNAQDGISLLQTAE"
BASE COUNT         2376 a         2373 c         3545 g         2238 t
ORIGIN      
        1 ctaaagtaag ggcggggcca tgccgataca taagttagga ctcaagagag gtgatggcct
       61 tgaaaatatc cggaagccgg ggatttgatc ggatcaacct atactcccaa ctggcaaaga
      121 aggctgaaag ggcagaacga acgggcgagg tgaagcccga tgaggttgag atctcggata
      181 ggggcaagga gattcaccac gccatgcagg ctctgtcttc cgtgtccgat gttcgggacg
      241 acaaggttga ggctgttcgt tcccgcattg aatcgggcac atacgaggtc agcggcaaag
      301 atgttgctcg caagctcttt gccggggctg tgagacgagg tgacctgggt ggattacgca
      361 aaggaaatgg cgaggattct taatcgcgag tttgcgctat gttcggagtt gctctacgtg
      421 gccagggcgc agcgcagcgt tatcacgtca ggggatgttg cggccctggc tgagctagta
      481 tcgagggcgg aagagataat aggagccatc agggatgagg agatcggtct tgctgatctg
      541 gcaaagcgtt gggcagacga gaccgaagtc gaagttgaag atgctgaaca agctctcgct
      601 gtcatgattg catcgttcaa ggaagaggag atggcggagt tcgaggtcct tcgggattcg
      661 atcgccggca tcctggcgga gatagactcg gcgaatgcaa tcaatgcaat gctcatccaa
      721 gatgcaatcg aatatataga gaataccgtc aggctgatag ctgaggcgga tgggaccaat
      781 tcggtctaca gccgcttggg acgcatggac aagaaggctg tgtcttcggc agtggacgat
      841 acggcttagc tgtgtcagat ggggtgaagc tcaagtgtac tctacattcg ttggactgga
      901 gactgcgcgc agggctctct acgcccagcg ggcggcgata gacataaccg ggcacaatgt
      961 ctctaacgtg aacacgccgg gctacagaag acagaggccg gtcattgagg cgactccccc
     1021 cgcgccaggg gcgcttcagc ccggaacagg ggtgaaggtg gccgggattg agcggatgcg
     1081 cgaccaattc gtggacacgc agataaggca taccctggca tctttgggca ggtgggaagc
     1141 gcgccaggag attctcgagg agatccagga agtattcaag gagccgagcg atattggcat
     1201 ctcggcgatg atggaccggt tctggatatc atggcaggag ctttcgggca atgccactga
     1261 tccggggagg cggaccagtg ttcagcagga ggctatttcg ctggcgtccg ccttcaactt
     1321 ggctgccgca cagctcaatg agacgaaagc agacattgat cagaatatca agtcgcttgt
     1381 acatcgagtc aacacgaccg cttccagcat tgcggagctc aaccgggaga ttgtcagggg
     1441 aaatgtagtc ggcgaaaacg tagcggactt gctggacaga cgggattatc tagttcagca
     1501 gcttgcgtcc gatgtcgacg ttgcggttgt ggaagacaat agcgggctcg tgcatgttac
     1561 tgtcgggggc aggatcctgg tggccgggac tgatgctagg gaattggctg aagacgccct
     1621 gattacgggt ggaaggataa agggcctcaa tgaggcggcc aacatgataa tccccggata
     1681 tctcaaggaa ctagacgatc ttgctaatgc gctcattgag aaaatcaatg agcagcacat
     1741 ggccgggtat gacctctttg gaaactccgg ggacgtcttt tttgaaggag agggcgcggc
     1801 ggggattcgt ctttcagaag agatagaggc gaatccgggg ttgattgcgg cggcagatca
     1861 gactgacagc cccggcaatg gcaggaacgc gttggccatg gctcagctca aggatgggct
     1921 cactatggtt gggggaacgg ccacgttccg cgacttttat ggttcgattc tgacaggcct
     1981 cgggcttgag acccaggaat cgatgaggtc gcgagaggtc tacgaaggtg tgctggagca
     2041 gcaaaaagcg aggcaggaat cggtatccgg agtttcgctg gatgaagaga tgatcatgct
     2101 cataaagtac tcatcagcgt ttgacgcggc tgctcggttg gtgactgcgg tagacgagat
     2161 gctcgacagg ctcatcaacg gaacggggct tgtgggaagg tagaggcggg cgcagagtgg
     2221 cacaggccct ctggcgcggg tttgagcgca gcacatagat gagaaagggt gttagggcca
     2281 tgcgggttac gaacaatgtc atgatggagc gggccatgcg caatgtgaat gtggcctttg
     2341 aaagatatgt gaagcgtcag gagcagctgg catcgggctg ctcgataaac aggctttctg
     2401 acgacccgca cggcgctgca aaggctgtaa ggcttaggtc taccctcaaa gggctcgaac
     2461 agtacaagac caatgcagac atcgcggata cgaagctcag ggcttatgac ggcgttcttg
     2521 acaaggtagg cagtattatg aaccgcctta gggatcttgt ggggcgcgcc gccagcgata
     2581 ctgtgactcc gacagaacgg gacgggatag ctgtcgaggt tagggagctg ttcgaggagg
     2641 ttgtcaacgc aggaaacgag atgcacaatg gggagcatat gttcgccggg cacaaggttg
     2701 gacaggcccc cttcaaggtg gagcgggacg cggatggcag ggtgactggc gtgttgtacc
     2761 acggcgattc cggcaaggac gcaatagaga taggccccgg gattgtgatc tctggaagcc
     2821 ttgccgggga tgaagttttc atggcggacg gtgccaatgt tttcgatgcc atgatcgaca
     2881 ttgaaaggca cctccgggaa aacgatgtgg agagcctgtc ggaagaggat cttgggcgtg
     2941 tggatgcagt agttgacaac attctcaagc atagggccga gatcggcgga aagggcaagc
     3001 gccttgaact cgtaatggac aggcttgaga gtgattccat atctattgaa ggccttctca
     3061 gcaagactga ggatatcgat ttcgcagagg ctataatgcg cctgtgtgag caggagatag
     3121 tttattatgc tgctctgtcg gcagcttcga aatcggtgca ggcaggcttg ctgcaatttc
     3181 tgagataggg gggcgtcaca agtggaacat ggattcgatg cagaagagat tcagcttgta
     3241 gtcttcgagc tggctgatga atcctatggc gtggagatct cccgggtgca ggacatcaac
     3301 aggatgcagg agataacgga gatacctcat gcgccggaat cggtagttgg cgtcatcaat
     3361 cttcgcgggc gggttatacc cgtagtcgac ctcaggacga ggtttgggct tcctcctgcg
     3421 gagcacacga agtctacgag gatagtcgtt gtgcagatgg gagaggagcc gatagggatg
     3481 attgtggatg cagtctccca ggtgcttcgc atacccacga agataatcga accgccttct
     3541 cctgttcttt ccagcgttga ttcgagatat ctgcggggta tagcgaaact cgaggacgag
     3601 ctcgtgatat tgctcgacct cgactacgtg ctttccaaag acgaacagga gttcatcagc
     3661 gaggcaatgg ccgagagcgt atagggggca gtccgatgct ggaatttccg ggcatgacgg
     3721 ccgatgacat caaggtgttt ttgatagaag cagaagagca acttcagtcc ctcgaagacg
     3781 gtttgctcga gttggagcag gccggcgagg acgatgaggt cattgggcaa atattcagag
     3841 ctgcgcatac tctcaagggt tcgtctgcca cacttggcca cgacaaaatg gctaatctga
     3901 cacattctat ggagagtgtg ctcgacctgg ttcgaaaatc gcagctcgag gtgagccctg
     3961 atctcatgga tgtcctcttc gaatgcttgg acgtgctcag agtgctcaat cgtgaagtcg
     4021 agacgggcca agagagcgga gaggacatta ccgtgcttac cgggcgcctg ctcgacataa
     4081 tcgcatttgc cgaagaggga ggctcgggtt gcatcagtgg tgaaagcgaa gcggggggat
     4141 ccggcgccca gtctacagtg gctgccgttg atggcggcat tatgcacggc ccgggcgatg
     4201 tggagctttg catcgctttc gcagatactt gtcagatgcc ttcggtcagg gcttaccagg
     4261 ttgtcgatag gctgtcggtg atggggaata taatctatac caggccttcg atggaactga
     4321 tcgaggcggg gcaggacata ggtgagctga ctgtggccat tgagtgggac ggcgattcgc
     4381 cgcagcccct tgttgaggcc gctatggatg tttcagatgt agagtcggct ctggtagttc
     4441 aggtgaggcc cgcaggctcg ctaaagggag aggccgatgc tgctgcggag gcgtcgcgcg
     4501 cgactgtggc ttcagggccc aaggaaagtg agcccacggg tgttgaggct gggcgtaagg
     4561 ccggtgtgaa taggggttca aggtctgcaa cgtcgactcg ggccactact gtgagggtgg
     4621 atgtggcgcg gctcgacgcg ttgtcgaatc tcgtggcgga gttggtgatc gaccgcacgc
     4681 acctggctca gctcgaatcg aagttggcgg agaaacatgg cggggacgag cttgtggcgg
     4741 agctgaacag gacatccact cacataggga ggctcaccac tgagcttcag gaggccataa
     4801 acaaggccag aatgttcccg atagacaatg ttttcaggcg ttttccgaga atggtgaggg
     4861 atctcgcgca gagccaggga aaagagatcg acttcatcat agagggtgaa gatacagagc
     4921 tcgacagatc agtctcggag gagatagggg atcctctgat ccacttgatc cgaaacgctg
     4981 tgggccacgg tatagagacg ccggaggaga gagtcgccgc gggaaaggcg cccaaaggga
     5041 ctgtgaggct ctctgctttt catcaggaaa attacatagt catagaaatt gccgatgacg
     5101 gacgggggat agatcctgag gcggtcaagg aatccgctgt ccgaaaaggc ctgatctcgg
     5161 aggaggcagc ggcgaggatg tctgaccggg acgcggtcaa cctcatcttt tcgccggggc
     5221 tttccactag caagaaggtg gatgatgtct ccgggcgtgg cgtgggcatg gatattgtcc
     5281 gaaagaacat agaacggctc aatggctcca tttccataaa tacacgtttg ggagaaggct
     5341 ccacttttac tgtgaaactt ccgctcaccc tggcgataat cagggcgctg ctggtcgcct
     5401 acgattctcg aatttacgcg attcctctgg cttcggtgat ggagatcact aggatagagc
     5461 ctactgacat ccatacggtg cacaagcgcg aggccatacg gctgagggga gatgtaatac
     5521 ccctgctatg gctgggtgag atcttttcca aaagaggcga ggagcgcggg gcggctgaat
     5581 ggatggcagc tgacggtcgg cctgaggatg gcgctgcagg agaggcgcag tcgagacgga
     5641 tgttcgcggt gatcacctcg tctcatgaag gccaagtcgg cctcgttgtc gattctttgg
     5701 tgggcgagat ggagatagtc atcaaaagcc tgggttcttt tattggaaac attccggggg
     5761 tatccggcgt tactatcctg ggagatggga gggtagcgct gatactggat attcccagcc
     5821 ttgtcaggag agtggtggaa gagagaaatg gcgctgaacg gggctaaaga tgtagtaccg
     5881 atcggaattg gcgagatgca tgtgtcggac aagccagggg atgttcttgt tgcatacggg
     5941 ctcggatcgt gtgtgggcgt ggtcattcat gaccctatcg ggaaggtcgg aggaatggtc
     6001 catgtggctt tgcccgagtc gctttccgcc ccaaggagcg aggaacgtcc tgatgcgaga
     6061 tacgcggatg tgggcgttcc catgctcgta ggcgaggtgc tgaggctcgg aggaatgaag
     6121 gcgaggctcg tggcccggat cgccggaggg gcaaagatgt ttgacgtcac acctgagttg
     6181 gaattcatgg atatcggggc gcgcaattcc gtagctgtaa gggcggagct tgaggcgatg
     6241 gggattccga ttgtcgccga ggacataggc ggggccgacg ggcgcacact tcgctatgtt
     6301 gtcggcaggg agcaggtttt tgtccgaaag cttgggatgc gagaggtgga gctttagctt
     6361 cagtgggggt gtttggcagg tgccgactgt ccttgttgta gatgatgctg ctttcatgag
     6421 aatgagggcc gggaaactct tgagccagag tggctacgag gtgattgagg cagaaaacgg
     6481 gctcgatgcg ttaaacaagt acacggagta cgagcctgac ttggtcctta tggacataac
     6541 catgcctgtt atggacggtc tgaccgcggt gcgggagata cgccaggtcg atccggaggc
     6601 gaagatcata atggtatctg ccatggggca gcaggctatg gtgatcgagg cgatcaaatt
     6661 gggggccaag gatttcatcg tgaagcctta tgagcctgat aatgtcctgg cttcggtgaa
     6721 gaagttgata ggctaatgat gcccatatca gtgctggttg tcgatgactc tgcattcatg
     6781 cgcaaggtcg tatcggagat gattgcctcc gatccggagc tttctgtggc gggcacggcc
     6841 agggacggca aggacgccat agagaaggct aggtccgtga gacctgatgt gatcactttg
     6901 gatgtggaga tgcccgtcat ggacgggctc gaagctctgc cagtgctagc cggggagctt
     6961 gggataccag tggtgatgct ttccagcctc acgcaggagg gggcggaggt aacccttcgg
     7021 gcgctcggcc ttggagccat cgattttgtg cccaagcctt caggcgcaat ctccctcgac
     7081 atcgacgttg tcagagaaga cctgctagcc aaggtgaagg cggcagcgaa ggtgtcgtct
     7141 gcacgggtgc gagggcttgc gagggacgcg cgggttccat tgacggggtt gggccgcgag
     7201 gttaaagagg ccaagtccgg ggcgagggcg gcccgggcaa gaaatgtagt tgtgatcggg
     7261 acttcaaccg gaggtccgag ggcccttacg caagtggtgc catggcttcc tccaaatctg
     7321 cctgccgggg tgctcattgt gcaacacatg ccggcggggt tcacgaagtc tttggccgac
     7381 aggttgaacc agacgtcgaa gataagggtg cttgaggccc aggggggcga ggccataact
     7441 gaaggtgcgg cgctcatagc tcagggagga ctgcatatgg tggtggagcc tgatggtact
     7501 gttaggctag actcgagtcc gcccatgcat ggtgtgaggc ctgccgtcga tttgactatg
     7561 aattccgccg tcgacctttt tgggaaaaag acggtgggtg tgataatgac cgggatggga
     7621 tctgacgggg ccgacgccat ggcgcgggta aagcggatgg gcggcaagac cgtgtgcgaa
     7681 cacgagtcca cttgcgtggt atacggcatg ccaagggcag ttgttgagca gggagatgcc
     7741 gacagggtgg ttccgctccc taagattgcc gatgagattg tagatgctgt tctttcatta
     7801 taatcactga agttatgctt gagtgagccc gtgaggtatt gcatatgagc cttgttgaca
     7861 acgcagatta cgaggtcttc cgcaggaagg tacttgcgct gaccggaatt gacctcggac
     7921 tttataagtc ccagcagatg cagaggcgca tagggagcct gatggaaagg gctggtgttt
     7981 ccacgttcgc tgcctatggc gacctattgg ggcgcgatcg ggcagagctg gatcggttct
     8041 gcgactacat aacgataaat gtgtcggagt ttttccggaa tcccgagaaa ttcgatgaac
     8101 ttgagcggga gatactgcct gagctccttg cgaggtctcg aaacctcagt atttggagcg
     8161 cagggtgttc caatggtgca gagatctatt cggtggccat gatccttcag gagatagctc
     8221 ctagaggata ccatcgcctc ctcggaaccg acatcgatac cgggattctt tcaaaggcac
     8281 tagcaggagt ctatggccca caggacgtgc gaaacgtgtc ggcggcgagg atggccaagt
     8341 actttaaggc ggacggggag tcgtacgtgc ttttgcctga aataaggtcg aaggtgcaat
     8401 ttaagatgca caacctgttg tcggatccct ttgagagagg ttttgacctg ataatctgca
     8461 gaaatgtcgt gatctacttc acagatgaag cgaagcaaga tctttacggc aagttctggg
     8521 aaagcctcaa ggatgacgga gtgttgttta tcggagggac tgagactatt ctcaatgcaa
     8581 aagacatagg atttgcgccg atggcgtcgt tcttctataa gaaggttcct gttcggaagg
     8641 aagttggcgt gggatgagaa ggatagcagt ggagcatatg aagccaggaa tgacgctggc
     8701 tgaagggata tacaacggct cgggcgtgac tcaggtttcc ccaggctcgg ttctgagtga
     8761 aacggcgatc gccaggatcc ggcagcttgg catttcacat gtcttcatag aggatcctcg
     8821 cacggatgat ctggtcatac gggatgcggt cccgtggaat gtggctgcta ggggcaggga
     8881 gttgctgcgg gatctcgcgg aacgcgttcg ggaggccggg ggtccggacc atgtcaaagt
     8941 gcccttcgac gatctgctcg agctggtcca tgatatcgag gacgggctgc agggtaccga
     9001 cttgaatgtc ttggagatac ccctggtaac ccgaccagag gactatcggt atgcttatcc
     9061 cattaatgtt gcggcgttgt ccatgttcgt cactgccaag gcggggttgc cgcggcgcac
     9121 gaccgatgtg gggctggcgg cgcttctgct cgacataggc atggagcttg cgcccgagga
     9181 gcccgagaag catccggaga tgtcccttgc agtgctcagg caggacgaga ggttttcggc
     9241 ctacagcagg gccatagtat ttcagcatga ggagaggcat gatgggagcg gatatcctcg
     9301 ggggattgcc ggagacgaca tcgaccccct ggcgaaaatc gtatcagttg ccgatatgta
     9361 ctgtagcctc atttctgagg gcaaccccat gggcgagagg cttgccccgc aggaagcctt
     9421 cgattacgtg accagcgcgg ccgggttcga gttcgaacgc actacggtgc tcggggtgat
     9481 gcagtatgtg gcgctgtacc cggtgggcac gatggtgcgg cttaacaccg gggagtgtgg
     9541 tgtagtaacc cgggtctaca aagggctgac aacgaggccc acggtgcggg tgctctatgg
     9601 tgcagatggt gtggaggttg cgcggcccta tgagatggtg ttggcggagc cggagcacca
     9661 gacggtgctg gttgcagggg tctgcgaaga ttagaaaggg cttgggggga tcgcaatgaa
     9721 ggttcagagc tcccggtttg gcaccctgac cgtggatggt gacaagatta tgagttttcc
     9781 aaaagggata cccgggtttg aggactctcg aaggttcttc ctattggatc atccggggag
     9841 cgcgaaggtg aagtggctac acagtgcaga cgatccgggg ctggcgttgg tggtggcaag
     9901 tccctttgag ctttttgcaa gttacgagcc ggaggtgccg gatggggtag cggaggcgct
     9961 cggggccgaa gggcctgagg acatgttggt gcttacggtg ctctcagtgc atgcggggaa
    10021 cccgcccaag gtgacggcca atctcttagc tccgattgtg gtatgctccg ctaccatgcg
    10081 gggcatgcag gttgtaatgg aggccggcaa atacggcgtg aggcacgaaa tggagcttgt
    10141 accggaaaaa tgtgaagcgg gggctaaatc ttcggtcggt tagtgccgat agagatattg
    10201 aaggcggcta tagagcctaa gtcctgcagg caaaggcata agccgtggcc tgccgcaagg
    10261 atgcggcggc cagtatagac actacaagga ggtagacata attgaggatt aaccacaacc
    10321 tttctgcgct caatgcgtgg aagaacatga gtgtgaacga cagcggtcag aacaagtcgc
    10381 tcgagaaatt gtcaagtggt ttgaggattg gaagggcggc ggacgacgca gctggactct
    10441 cgatttcaga gaagatgcgt ggtcagattt caggcctaaa ccaggccagc cggaacgccc
    10501 aggacggcat ctccctcctg cagaccgcag aa
//
LOCUS       DAOSAI010000035        10188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4329_length_10188_cov_3.861739, whole genome shotgun sequence.
ACCESSION   DAOSAI010000035 DAOSAI010000000
VERSION     DAOSAI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4329_length_10188_cov_3.861739"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PLM83_02405"
     CDS             complement(<1..699)
                     /locus_tag="PLM83_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK70293.1"
                     /translation="MGRLELTIIGNQGPYPGPGGACSGYLVRSQGATVLIDCGPGVMP
                     RLQQHIDVSSLDAVVISHFHPDHYSDFLTMRYAFQYIESTGRMGCPVTLALPAVDAEW
                     FLREIIGEDYKPLFRIVPIEHGSRLEIGGRVRGGDCGLVLDFRLMSHAIPSFGTIVYA
                     SQPEHGSRAPLAYTSDTGLCESLEEMAASSHTLLVEASMQEGDAQRSSAGHLAAAEAG
                     RIASEHGVARVLLTH"
     gene            complement(720..1571)
                     /gene="murI"
                     /locus_tag="PLM83_02410"
     CDS             complement(720..1571)
                     /gene="murI"
                     /locus_tag="PLM83_02410"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOK70294.1"
                     /translation="MQYRSVGVFDSGVGGLTVARAIMEVMPGQDIVYYGDTARMPYGD
                     KPPEEIVRYARQIISFLVERGVSAVAVACNTSSAIAVPVIGRDFDVPVVGMIQCGAAA
                     AAEASRSGRIGVVATANTVRSGAYSRAIANLRPCASVFEQACPMLVPMVERGEVSGPR
                     VREVVAGYLDPLLEAGIDTIVYGCTHYPFLEDEIRAVAGSSVALVDPAIKVASAVKTA
                     IGEDGQMVKMRGGLPSFVTSGDPCAFSQLVRSLLGWNVQEVWHVDLDAAEASAGKCSL
                     PGRADLE"
     gene            complement(1625..3580)
                     /locus_tag="PLM83_02415"
     CDS             complement(1625..3580)
                     /locus_tag="PLM83_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HOK70295.1"
                     /translation="MRCIERRLRVLIIMLTAFVCFSGSALADNPWVLRIAQGNGFVEI
                     ADGIVCVDNGPFVLMRSLAAGLGGDLTWIESTKESVFTTDRTVAVIKAGQRTAYINGQ
                     EVELSSLPDLSTGRIAISVKDLALLGLKAGVDFDLGTVTVAYQRTSVSSASFSVEDGR
                     VTIIVEADGGFECSDFILRQPDRVVVDIHDAVLSPSFQGFDVSYGAVQRVRAAMNRPG
                     VVRVVVDLNAPLGYTISYEPGMPSRLAIRFNTLVGEIRLDQDTSIPRLRVKGDAPIEH
                     GSPVDDGGGCWHIDMKGTTAAGGITALRDEIGPVNWVKASQPEPTTARLEFGLKKGYS
                     PIIRSPESNANELLIDFAITVSKMDVFEEPGCTVIQIVTSGPVEFKTFRLRSPERIGV
                     DLPGVWAGGYGSLPVESAGVEKIRFAQFTADVGRIVLDLQPEATRWAHSVEISADRTT
                     ATIRVGSSPIYGRTVMLDPGHGGSDPGTVHDGLYEKEINLDIALRARDLLVKAGATVV
                     MTREDDSTVPLGDRPRMANEVKPDAFLSIHCNSVLNVFPCGTETYYYNMVDYSQELAT
                     QIHYCLLKEIMLVDRKVRRKDYYVLKETQVPAVLVEVGYMSTSEENEKLRQPEFRQKA
                     AQGLVNGLAAYFDSDVYARWRTEVRAK"
     gene            3767..4840
                     /locus_tag="PLM83_02420"
     CDS             3767..4840
                     /locus_tag="PLM83_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOK70296.1"
                     /translation="MKVGFSGRPAPPREMRAPFGSTPLERMQRYVSACSEMELGCLEV
                     PATLVFGKGDWRNQFLSACTEPVRAEPKRNPSPVDVLVRVRFTDGVDPRAAESSPCTA
                     ATLTYARHLIRLFGGALVTLQFKPSHASLIGKAPVPTGESGAAKVGLVVDFADQSGVQ
                     AAARIVRKSRHYIMLPIVAITLDNMIKAERQIMRALTVCYNENGGGEIFVYLALPQSG
                     AAAGRVASRNWEAILETIANFECSRGSDVGLLFSGPGSEKHARAMAQMVSALYPPRCG
                     AGVESDLVTRIGSTVRIRDVELGLDMEYFIVAPEQASAKKGFLSSESPVGRAIIGQPR
                     GSTVQVAAPGGPILYELLEVLNN"
     gene            4940..5068
                     /locus_tag="PLM83_02425"
     CDS             4940..5068
                     /locus_tag="PLM83_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70297.1"
                     /translation="MQIEAALFTVMIIDTKLGKTSPETPVGIAEAGFQFQSDVDYT"
     gene            complement(5157..5567)
                     /locus_tag="PLM83_02430"
     CDS             complement(5157..5567)
                     /locus_tag="PLM83_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70298.1"
                     /translation="MPGQSEFEKIISAASLQVESISDNVYFGEGHDWKEIVRQLQEVA
                     GNLDIVLYGRNVPWSFYKRAQELRAEISYAINIANQRERYDKILELEEVLRSETSDAG
                     WRAYLSIDVLSLDPIVWEPVCMATCCFQRRHSLM"
     gene            5816..6196
                     /locus_tag="PLM83_02435"
     CDS             5816..6196
                     /locus_tag="PLM83_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOK70299.1"
                     /translation="MNERLINVGKRIREARLRQGMSQEELSEKCCMNPSYIGQIERGE
                     KSPSLNALYSIADGLDVNIRSFFESPETEEELAIQRLIDTIANKPPYQIDKIRRISEI
                     LLEDEVGMPSSTKPQTRDHSKPSR"
     gene            complement(6346..7512)
                     /gene="glgA"
                     /locus_tag="PLM83_02440"
     CDS             complement(6346..7512)
                     /gene="glgA"
                     /locus_tag="PLM83_02440"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOK70300.1"
                     /translation="MKSVNSLGVTMMTREFPPNIYGGAGVHVDYLSTHLADLMNVEVR
                     CFGEGAQTEDAIRIRSYSPWLEMSKGSDPKLQKVLDPLSVDLAMVKDPIETEIVHCHT
                     WYTFFAGYLAKTMYGSKLVTTVHSLEPLRPWKEEQLGRGYSVSSWMERIGIENSDCVI
                     AVSKEMRQDVIEHYDVEPEKVVVIHNGIDLSGYRRTDDTSALEQYGIEEPYVLFVGRI
                     SRQKGIMYLLDAAPMIRPGVKLVLIAGTPDTKEIEEELAARVKDLPNVVWINRMLPRE
                     HVIQLYSHASVFACPSVYEPFGIINLEAMACEIPVVASAVGGIPEVVVDGETGFLVPP
                     GDPESLASAINRVIDSEKLHTQFGKAGRRRVERFFSWEAIAEKTKDMYLEILSE"
     gene            complement(7509..9086)
                     /locus_tag="PLM83_02445"
     CDS             complement(7509..9086)
                     /locus_tag="PLM83_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOK70301.1"
                     /translation="MAEFSPEKAYSAVKPSEFARQRWFRHKLSHVTDFEILDCAEFES
                     GVTADQCLVLGILRFEGAGDYFVPVVAESLLPGSGEKCPKAYGVIEDGSSGEKAVVYD
                     GAFHPMYVNTLLDLIESDATIPAVKGQFRFRKIVSSRAERGWEISQVGDAYTNSVVIV
                     QRRSALKTFRVMTPGINPDVEVGIALATETDFRETPRVQGEVSYVDSCGVEYSVAVQT
                     EEVPNIGDAWELVEIALTEAMKSVAHGLKEGDAKFSDFDGILSADASRRLGATIGRFH
                     QAMASIDAPGFGVRRAGPGDIDMWRRNARARACGALADIIHALDCGLLADDADAAYAA
                     ERVLAAEESILEAAERMLWGLAPDLNGAGMVIRCHGDLHLGQILVRPDGSYAILDFEG
                     EPMASLEERRALHSPARDVAGMLRSYSYAANAAMAAARANCEAECLCKLESLVNEWES
                     RARTAFLEGYFGEACSGVGNLLPGDFRARCALVSYFELDKAMYEAGYELNNRPDWVVI
                     PLKGILQIMERRGGNTR"
     gene            complement(9196..>10188)
                     /locus_tag="PLM83_02450"
     CDS             complement(9196..>10188)
                     /locus_tag="PLM83_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOK70302.1"
                     /translation="YSTIEGYTPLREWLASAVAERGMKATTEQVLITSGPQQGIDLAA
                     KCFINPGDCVLVERPTYLAALTIFKTYEVEFGCVDSDDYGIIPESFEEAIVRHNAKML
                     YVVPTFQNPTGVTLPAERRKAIAEIAGKHGVIVVEDDPYAKLRYDGEPVPSIASYDEN
                     DVVIFLGSFSKFISPGLRVGYAISPPEIHEKLAQGKQTTDFHTSNLSQRMIYEFCTRG
                     YLEPHLKSIRKDYAIRRDAALEALEKYFPEGTRWTHPQGGLFIWVVLPEHIDTTELLP
                     KAAAKNVAYIPGVPFYADGGGKNAMRLNFSNASLEQLDVGIKCLGEVISEAMKA"
BASE COUNT         2432 a         3017 c         2391 g         2348 t
ORIGIN      
        1 atgcgtgaga agcactcggg cgactccatg ttctgaagca attctgccgg cttccgcagc
       61 ggcaagatgg cctgcagaac tccgctgtgc gtcgccttcc tgcatagatg cctcaaccaa
      121 tagtgtatgc gagctcgcag ccatttcctc caaagactcg cagagtccgg tgtctgacgt
      181 gtaggcaagc ggcgcccgag acccatgctc aggttggctt gcatacacga tcgtcccaaa
      241 gctagggatg gcgtggctca tcaaccgaaa gtccaggacc agcccgcaat ccccgccgcg
      301 cactctgccg cctatctcca gacggcttcc atgttctatg gggacgatgc ggaagagcgg
      361 cttataatcc tccccaatga tctcccgcaa gaaccactcg gcatctactg caggaagcgc
      421 cagggtcaca ggacagccca tccggccggt tgactcaatg tactgaaacg catatcgcat
      481 tgtgagaaaa tcggagtaat gatcggggtg gaaatggctg ataacaacgg catccagact
      541 tgaaacatcg atatgctgct ggagcctggg catcaccccg ggtccgcagt cgattaggac
      601 cgtggcgccc tgggatctca caagataacc cgagcatgcc ccgccggggc ccggatatgg
      661 gccctgattg ccgatgattg tcagttcaag cctgcccaac tccatacctc ccggtgtttc
      721 tactctagat ctgctcgccc aggcaaactg cattttccag cgctggcctc agccgcgtca
      781 agatccacat gccacacttc ttgcacgttc caaccgagca gagagcggac tagttgggaa
      841 aaggcacacg gatcgccact ggtcacaaac gagggtaatc ctccacgcat tttcaccatc
      901 tgcccatctt ccccaatggc tgtcttaaca gccgacgcaa cctttatggc cggatcaacc
      961 agggcaactg atgaaccggc cactgccctt atctcatctt ccaaaaatgg atagtgggtg
     1021 caaccgtaga cgatagtatc aatgcccgct tccaacagcg gatctaggta tcctgcaaca
     1081 acctctctaa cacggggacc tgaaacttct ccacgctcga ccatgggcac gagcatcgga
     1141 cacgcctgct cgaaaacact ggcgcagggg cgaaggttag caatcgcccg cgaatatgcc
     1201 ccgctccgca cggtgtttgc tgtcgcgaca acgcctatgc ggcccgatcg agaggcttct
     1261 gccgctgcag ctgctccaca ttgaatcatc cccacaacgg gaacatcaaa atcgcgcccg
     1321 ataactggca cagcgatggc agacgacgta ttacatgcta ctgccacagc gctgacgccg
     1381 cgttcaacca agaacgagat tatctgccgc gcatagcgaa ctatctcctc gggcggttta
     1441 tccccgtagg gcatccgcgc agtatcccca taatagacta tgtcctggcc gggcatgacc
     1501 tccataatag ccctggcgac ggtgagaccg cccactcctg aatcaaaaac gcctacactt
     1561 ctgtactgca tgtgattatt atactcctgc cgcgcaaaga gttacaacgt catccaccag
     1621 accctcactt ggccctaact tcagtccgcc accgagcgta gacgtcactg tcgaagtaag
     1681 cagcgagccc atttaccaac ccctgagcag ccttctgcct gaattcaggc tgacggagct
     1741 tctcgttttc ctcacttgta gacatatatc ccacttccac cagcacagct ggcacctgtg
     1801 tctccttgag aacgtaatag tccttgcggc gaacctttct gtcaaccagc atgatctctt
     1861 taagcaagca atagtgaatc tgtgtggcta gctcctggct gtaatcaacc atattatagt
     1921 agtaggtctc agttccgcac ggaaaaacat tgagcaccga gttacaatgg atcgataaaa
     1981 atgcgtcagg cttcacctca ttggccatgc ggggcctgtc gccaagggga actgtagagt
     2041 catcttcgcg cgtcataacc acagtggccc cggctttcac caataggtct ctcgcacgga
     2101 gcgcaatgtc aaggttgatc tccttctcat agaggccgtc atggactgtg cccggatctg
     2161 atccaccatg gcccggatcc agcatgacag tccgtccata aatgggtgag ctccccactc
     2221 tgatagtggc cgtcgtcctg tcggccgaga tctcgactga atgcgcccac cgagtcgcct
     2281 cgggctgcag gtccaacaca attctgccta cgtcagcagt aaactgagca aatctgattt
     2341 tctccacccc agcggattcc accggcagcg aaccatatcc ccctgcccac acacccggaa
     2401 gatcaacccc aatacgttcc ggactcctga gcctaaaagt cttgaactcc acgggccccg
     2461 aggtcacaat ctggatcact gtgcacccag gttcctcaaa cacatccatt ttcgatacag
     2521 tgatggcgaa atcgatcagt agctcgttgg cattggattc aggtgaacga ataattgggg
     2581 agtatccctt cttcaaaccg aattcgagtc ttgccgtggt tggctccggc tgagatgctt
     2641 taacccagtt aacagggccg atctcgtccc gaagagcggt gatcccacct gccgccgttg
     2701 tgcctttcat gtcaatgtgc caacaaccgc ccccgtcatc cacaggagaa ccgtgctcta
     2761 ttggagcgtc gcccttcact cgcaacctgg gaatgctcgt atcttggtcg agtcggatct
     2821 ccccgacgag cgtgttgaag cgaatggcca atcgggacgg cattccgggc tcatacgata
     2881 tggtgtatcc caatggagca ttgagatcca cgaccactcg gacaacccca ggcctgttca
     2941 tggcggctct tacgcgctgc actgcaccgt aggacacatc gaacccctga aaggacgggg
     3001 acagcacagc atcatgtatg tccactacca cccgatccgg ttgacgcaga atgaagtcag
     3061 agcattcaaa accgccgtcc gcctcgacaa tgatcgtcac tcttccatct tccaccgaaa
     3121 acgaagcgga tgaaacagaa gttctctggt aggcaactgt gaccgtcccg agatcgaaat
     3181 cgacgcctgc tttcaagccg agcaacgcta ggtccttgac gcttatcgct attcgcccgg
     3241 tggacaggtc aggcagcgac gagagctcaa cttcctgccc gttgatgtaa gcagtccttt
     3301 gtcccgcctt tatcaccgca acagttctgt ccgtagtaaa caccgattct tttgtcgatt
     3361 ctatccaggt tagatctcct ccaaggcccg cggcgagaga acgcatgagc acgaatggtc
     3421 cgttgtcaac gcagactatg ccgtccgcta tttcgacaaa gccattgcct tgcgcaattc
     3481 gaaggaccca tggattgtcg gctaacgcag atcctgaaaa acagacgaac gctgtgagca
     3541 tgatgatcaa caccctcaag cgcctctcga tgcaacgcaa tcgcacagcc ccctctgtga
     3601 acgtcctcaa tcactttttt ctactctttt cggcataatc ctctgcaccc acgatcaatc
     3661 gcttctggac gcttggcaat cgtggaacca cagatacaaa actgtgcgat tcaaatgact
     3721 atttctcatt tcagctggac atcataggct aaaggaggcg acaaaaatga aagttgggtt
     3781 ttcaggaagg ccggcgccgc ccagagaaat gcgggcgcct ttcgggtcaa cgccactaga
     3841 gcggatgcaa cgatatgtat cggcctgctc cgagatggag cttgggtgtt tggaagtgcc
     3901 agccaccctc gtgtttggca aaggagattg gcgcaatcag tttctctctg cgtgcacaga
     3961 gccagttcgc gcagaaccca agagaaatcc ttcgcccgtg gatgttctcg tacgtgtcag
     4021 gttcactgac ggagtcgatc ctcgggcagc ggagtccagt ccgtgcacag ctgctaccct
     4081 aacatacgcc agacacctaa tacgcctgtt cggcggcgct cttgtcacat tgcagttcaa
     4141 gccatcccat gcttctctta ttggaaaagc cccagttccc accggagaat ccggagctgc
     4201 aaaagtcggg ctggtcgttg acttcgctga ccaatcagga gtgcaagcag ctgccagaat
     4261 cgtcaggaaa tcgagacact atataatgct gcccatagtg gcgatcacct tggacaatat
     4321 gatcaaggca gaacgtcaaa taatgcgggc actaacagta tgctataacg aaaacggagg
     4381 aggcgagatc tttgtctacc ttgcgcttcc acagtctggc gcagcagctg gtcgtgtagc
     4441 ttctcgtaat tgggaagcca ttttggaaac gatagctaat ttcgaatgct ctagaggctc
     4501 tgatgtgggc ttgctttttt ccggtcctgg atcggaaaaa cacgcgcgcg caatggctca
     4561 gatggtctcc gccctttacc cgccccgttg cggagctggc gtcgaatctg acctcgtaac
     4621 gcgcataggc tcaacagtaa gaatacgcga tgtcgagctc ggattggaca tggagtactt
     4681 catcgttgcg cctgaacaag cgtcagccaa gaaggggttc ctctctagcg aatcaccagt
     4741 aggacgtgca ataatcggac aacctcgcgg ctccacagtg caagtggcgg ccccaggcgg
     4801 gccgatacta tatgaactgc tagaagtgct caacaactga caaagctaga tcttgcccgc
     4861 caggctaaac aagccatagg caagcaacgc gagaatgaac acctggctca ggggatttgt
     4921 gcatactcgg aagtgccagt tgcagatcga agcggccttg ttcacggtca tgatcatcga
     4981 taccaagctc ggaaaaacct cgccggaaac accggtgggc atcgctgaag cgggatttca
     5041 gtttcagtct gacgttgatt acacataagc agatctgact ggacttgcgc atctcagaca
     5101 tggcttgcct tcggccatgg ttatgcagcg gtcatccggc agttgtattc gacagttcac
     5161 ataagactgt gccttctttg gaagcaacac gtcgccatgc acactggctc ccaaacaatc
     5221 ggatccaaag agagcacatc tatgctgaga taagcacgcc accctgcatc ggacgtttcc
     5281 gacctgagca cctcctcaag ttccaggatc ttatcatagc gctcgcgctg gttagctatg
     5341 ttgatcgcgt aggagatctc tgcccgcagt tcctgtgcac gcttgtagaa gctccaaggc
     5401 acgttgcgac cgtacaaaac tatgtcaagg ttccctgcca cctcctgcaa ttggcgtaca
     5461 atctccttcc agtcatggcc ttcgccaaag tagacattat cactaatgct ctctacttga
     5521 agtgatgccg cactgattat cttttcaaac tcggactgcc caggcacttg aattcctccc
     5581 gtctccaatt ctttcatgct gatcctatca caaggcaata taatcttgta tcgcctcctt
     5641 cctaataccc atgtatgata tttggagtga ttttagccct ttccttacta gttctgtcat
     5701 atcgcttcaa gcaggccgta ggtgcctgtg gcactttttt tgccagcaat atgtgctgcc
     5761 agctgcttcc gccagattct tgtccgagtt accatcatca tggggaatgg ataaattgaa
     5821 cgaacgattg ataaacgtag gaaaacgaat acgggaagca aggcttcggc agggcatgtc
     5881 gcaggaggaa ttgtccgaga agtgctgcat gaaccctagc tacatcggac agattgaacg
     5941 gggtgaaaaa agtccgtcac tcaatgctct gtattccatt gctgacggtc tggatgtaaa
     6001 catcagatct tttttcgaat cgcccgaaac tgaagaggaa ttggcaatcc agcgcctcat
     6061 tgacacgata gcaaataagc ctccatacca gatcgacaag atcaggagaa tctcggaaat
     6121 cttgctcgag gatgaggttg gaatgccttc tagcactaag ccccagacca gagaccacag
     6181 caaacctagc cgttgatggt tttcccacat attgtccaca acaaaactag cctgggccat
     6241 tacataaccc aggctttttt ctttttcatc cgcgccaatt cttcttaata aagccggtcc
     6301 tcaattagac tcagctgagc agtttttctt ctccctctca gcgcttcact cgctcaggat
     6361 ttccaggtac atatccttcg tcttctctgc aattgcttcc caagagaaga agcgctcaac
     6421 gcggcgcctt ccagccttgc caaattgagt gtggagtttt tcagaatcga tgacacggtt
     6481 tattgccgac gcgaggctct caggatctcc cggaggaaca aggaaaccag tttccccgtc
     6541 tacaaccact tcggggattc cacctactgc tgatgctacg acaggaatct cacatgccat
     6601 ggcttccagg tttataatcc cgaaaggctc gtacacagac gggcatgcaa atactgacgc
     6661 gtggctatag agttgaatta cgtgttcacg cgggagcatc ctgttgatcc atacaacatt
     6721 tggaagatct tttacgcgcg cagcaagctc ctcttctatt tcttttgtgt caggagtgcc
     6781 cgcaatcagc acgagtttca ctcccggcct tatcatcggc gcagcgtcaa gcagatacat
     6841 aatccctttc tgcctcgata ttcttccaac gaagagcacg taaggttcct ctatcccata
     6901 ctgctccaga gcgctagtat cgtcggtccg ccgatagccg ctaaggtcaa tcccgttgtg
     6961 tatcacaaca accttctcag gttccacgtc gtagtgctca atcacgtcct gacgcatttc
     7021 ttttgagact gcaataacgc aatcggagtt ttctatcccg atcctctcca tccacgagct
     7081 gacagaatat cctctgccca attgctcctc tttccatggg cggagcggct caagggaatg
     7141 cactgtagta acgagcttcg agccatacat cgtctttgca agataacccg caaaaaacgt
     7201 gtaccacgta tggcaatgga ctatctctgt ctcaatgggg tcctttacca ttgccagatc
     7261 gactgaaagc ggatccaaca ccttttgcag cttcggatcg gatcctttgg acatctccag
     7321 ccaggggctg taactgcgaa tccgtatcgc atcttccgtc tgagccccct cgccgaagca
     7381 cctcacctcc acattcatga ggtctgcaag atgcgtcgaa agatagtcga cgtggactcc
     7441 tgccccaccg tatatgttcg gcggaaactc acgcgtcatc atcgtaaccc caagcgagtt
     7501 gacgctcttc atcttgtgtt gcctccccgt ctttccatga tctggagaat acctttgagg
     7561 ggtatcacga cccaatcagg cctgttgttc agctcatacc cagcctcata catggccttg
     7621 tccaattcga aataggatac taaggcacat cgtgcgcgaa aatcccctgg aagcaaattg
     7681 ccgaccccag aacacgcctc accgaagtat ccctcaagaa atgcggtcct cgcacggctc
     7741 tcccattcat taacgagact ctctagcttg cacaggcatt ccgcttcgca attcgcccgg
     7801 gcagctgcca tagcagcatt cgctgcatag ctgtacgacc ttagcatgcc ggccacatcc
     7861 cgggccgggc tgtgcaaggc gcgcctctct tccagcgaag ccatgggttc gccctcaaaa
     7921 tcgaggatag cataagaccc atccggccgc acaagaatct gcccaagatg aaggtcgcca
     7981 tggcatctaa tgaccattcc tgccccgttc agatcaggcg cgagtcccca tagcatcctt
     8041 tccgctgcct cgagtatcga ttcttcagcc gccagcacgc gctctgccgc ataagccgca
     8101 tcagcatcgt ccgccaacaa accacagtcc aaggcgtgta taatatcagc caaagcccca
     8161 cacgcgcgcg ctcgtgcatt tcgtctccac atatcgatat cgcctggtcc cgcgcgccgc
     8221 acgccaaagc ccggagcatc tattgaagcc atagcctgat gaaacctgcc aatagttgcg
     8281 ccaagacgcc tcgatgcatc cgcagacaga atcccgtcga aatccgagaa cttggcatct
     8341 ccctctttta gtccatgcgc aaccgacttc atcgcttcag tcagagcaat ctcaacaagc
     8401 tcccaagcat cccctatgtt cgggacttcc tctgtctgca cggccacaga atactccacg
     8461 ccacagctat ccacgtagga gacttcccct tggacccggg gtgtctcacg aaaatccgtt
     8521 tcggttgcaa gggcaatccc gacttccaca tctggattga tccccggagt catgaccctg
     8581 aacgtcttga gcgcgcttcg gcgctggaca ataaccacgc tgttggtata ggcgtctcct
     8641 acttggctta tctcccagcc tcgctcggcc cggctgctga cgatttttct gaatcgaaac
     8701 tgccctttca cagctgggat agtggcgtct gattctatga gatccagtag tgtgttgaca
     8761 tacatgggat gaaaagctcc gtcgtaaaca acagctttct ctcccgagga tccatcctca
     8821 attacgccat aagccttagg gcacttttct ccagaacccg gcaggagact ttctgcgacc
     8881 acaggaacaa aatagtcgcc tgctccttca aaacgcaaga ttccaagaac caggcattga
     8941 tcggcagtta caccggactc aaattcggcg caatcgagaa tctcaaaatc ggtcacatgt
     9001 gacagtttat ggcgaaacca ccgctgacgc gcaaattccg agggtttgac cgccgaatat
     9061 gctttctctg gcgagaattc cgccaaagca atacacctcc gcctaattgc aaaggacccg
     9121 gacacatccc gtccggcggg cgggcgcacc cgggtccata gctcttcaag atctagtctt
     9181 tccctcagca gcgttttacg ccttcattgc ttctgaaata acctcaccaa ggcacttgat
     9241 tcccacatca agctgctcca agcttgcatt gctgaagttg agacgcatgg cgttcttccc
     9301 accaccgtct gcataaaatg gcactccagg tatgtaggct acgttcttcg cagccgcctt
     9361 cggcagcagt tcagtggtat caatatgctc aggaagcaca acccaaatga aaaggccacc
     9421 ttgtggatgc gtccatcgag tcccctcagg gaaatacttt tcgagggcct ccagcgctgc
     9481 gtcgcgcctg attgcgtagt cctttctgat cgacttcaga tgcggctcga gatacccgcg
     9541 agtacagaac tcgtagatca tcctctgaga caggtttgaa gtgtggaaat cagttgtctg
     9601 cttgccctga gccaatttct cgtgaatctc cgggggcgat atcgcatatc ccacgcgaag
     9661 tcccggagag atgaacttcg aaaagctccc taggaatatg acgacatcat tctcgtcata
     9721 gcttgctatt gagggaaccg gctccccatc gtatctcaac ttagcatagg ggtcgtcttc
     9781 aaccacgatc acgccatgct tgccggcgat ttcagcaatg gcttttcttc gttcagctgg
     9841 taacgtaact ccggtcgggt tttggaacgt aggaaccaca tacaacattt ttgcattgtg
     9901 gcgcactatc gcttcttcga aagactccgg aattattcca tagtcatccg aatccacaca
     9961 accaaattcc acttcatacg ttttgaatat tgtgagtgct gctagatacg taggtcgttc
    10021 caccaacacg cagtcgccag ggttaatgaa gcatttcgcg gccagatcga tgccttgttg
    10081 cggtcctgaa gtgattaaca cttgttcagt ggtcgccttc atcccccgtt cagcaacggc
    10141 gcttgcaagc cattctctga gcggtgtata gccctcgatc gtcgaata
//
LOCUS       DAOSAI010000036        10071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4389_length_10071_cov_3.771765, whole genome shotgun sequence.
ACCESSION   DAOSAI010000036 DAOSAI010000000
VERSION     DAOSAI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10071
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4389_length_10071_cov_3.771765"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1438)
                     /locus_tag="PLM83_02455"
     CDS             complement(59..1438)
                     /locus_tag="PLM83_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK70303.1"
                     /translation="MRASVGRRMPIFIIMLILAASSGATADGTFGARWSIDTFADAAV
                     NVWKNSPQARQPLELALIGEGLSVPVRSASITISSGMSSASQLTESTGDRGDAEKVSA
                     KLPITDRISLNAAYDLNKSSGSLKLSYTLPHTWLAGSDEGIAQRLFGHSGASEDAKVS
                     TDMARMKLDASESSIKLKAQKAYIGALKSQKNRETAEEEWRLAQTALEIATKRCQVGL
                     STERDVEQAELSVLDAEIALVKAENEERWARRELSKLAGVDMLEAELEDLPQFSLDMP
                     SLDVMIEAAFESDVELAQAELGLDSSIRELEAIKSLLPTVSAEVQFPSGSSVPKFVAS
                     ATWSISLSRSLEAQKAEINVDQKRQSVTERREALAENVERSLEQLHVSVWSAAKWVKQ
                     LEQSQESYDTALKSYEDGELLLVDLERAQLNLKKARNSYIANWYGVWELWYTLANTCR
                     VEDLGMGNR"
     gene            complement(1461..2633)
                     /locus_tag="PLM83_02460"
     CDS             complement(1461..2633)
                     /locus_tag="PLM83_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70304.1"
                     /translation="MRPADYLRLALGGVTSNPFRSILTALGVIIGVSAVIITVSIGTG
                     AKVQTQSQIQRLGSNLITISSGYRSLRSVITSDILPIIENINTVGEVVPQVQRQSTVV
                     HGAEGLSTTVTGTSANYPSVRNLKLAQGSFLTDADIDKGSWNCVLGADLAEELFGDDN
                     PLGQKVRLDRVRFTVIGVAQRQGDSGFASSDNAMYVPYTTMQRRITGSKNINTIIAQA
                     VDASSMDLAYSQIYAMLMMATGDEDSFRISNQADMLEVASDVTDTMTLLLSGIAAVSL
                     VVGGIGIMNIMLVSVTERTREIGIRKAIGAKEHEILSQFMLEAAALSLAGGLLGIVVG
                     LVGSRIVSQVLSWPTAVDMKSVALGFTLSLIVGVFFGAYPAYKGAKLDPIVGLRYE"
     gene            complement(2630..3337)
                     /locus_tag="PLM83_02465"
     CDS             complement(2630..3337)
                     /locus_tag="PLM83_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70305.1"
                     /translation="MSLIELKEVSKTYSMGKGSIEVRALKAVDLAVDEGEFVSIMGQS
                     GSGKSTLLNIVGCLDKPTSGEYWLDGCLVGSLGSDELAEVRNKIMGFVFQSFNLLPKL
                     TAVGNVELPLVYAGVHPRIRREEAIRALDMVGLADRAFHRPNELSGGQQQRVAIARAL
                     VSNPRVVLADEPTGNLDTKSSYEIMDILADMNKRGITVVIVTHDPDIAAYTPRVLTFR
                     DGECIEDVRALEGGVDK"
     gene            complement(3349..4545)
                     /locus_tag="PLM83_02470"
     CDS             complement(3349..4545)
                     /locus_tag="PLM83_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK70306.1"
                     /translation="MKRSKTAVIIVLLIAVAGGAGFMWWNRSRSKPGAASNPYIPIEV
                     RRGELSTDIYATGNVQTIRTVDVYPTKAGIVAQVDVDEGTEVRAGDVLIRLERDELEV
                     QQTEAEVRQKRENLASSEVALEKIRSLHAKGAATEKELRSAESDVLQAKESYNAAALK
                     LDRLVTKASDSVIRAPISGVVSSVKATVGAGVSTSNPVAVITDLSDVVVRVTVDEYDI
                     GSVEVGQGAEVVMDALDDRKYAGNVCFVGKIGQNQSGVVVFNVDIRLDNPTQDVRPGM
                     SAEASIMVDRVENALIVPNSVLETRRGETVARIYNADGSVQLRPVIVGLATDGGTEIV
                     DGLNLGDMVAVPNPRAVSSNEDQGSAGREPEQGMPGMMPGMPGEAPTGVPGMGGPSGG
                     FAPPQR"
     gene            complement(5735..6241)
                     /locus_tag="PLM83_02475"
     CDS             complement(5735..6241)
                     /locus_tag="PLM83_02475"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOK70307.1"
                     /translation="MDAEYFVDLALEWGADHAVPFEISDIVFDPRTILKCAFGCGDWG
                     YGHTCPSRPLSLRPWEYEQVLRRYSWGIIIHSTDKRISQDVSFGIEREAFLRGYYLAF
                     SLSDCAICGQCAGFVGKPCANPQKARPAFHSVGIDVFKTVHRFGLPLDTLKSEDETPN
                     WYSAVFIE"
     gene            7173..7331
                     /locus_tag="PLM83_02480"
     CDS             7173..7331
                     /locus_tag="PLM83_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70308.1"
                     /translation="MRTLFGSRLRDTKHRRLLKAVVTANRLYGLMDTLMSAFLNNLTP
                     SEIQTLAA"
     gene            7527..8651
                     /locus_tag="PLM83_02485"
     CDS             7527..8651
                     /locus_tag="PLM83_02485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOK70309.1"
                     /translation="MIRRKTFPLVLISLSLILVLWAQPGAPGMSSRFSFAAVGDALIH
                     QGVYNAARVEDDAFDFRPMLRLIADRIARHDLAFYNQETILGGVELGLSTYPRFNSPQ
                     EVGDAFIDTGFNLVSLANNHTLDRGENAVLCSLEYWSGKESVLTAGSYFSQTDRNEIQ
                     TVEVNGITCAFLAYTTLTNGLRPPNGKAYYVNVYDPETARADVEKAKANADVVIVSMH
                     WGDEYVFAPTKEQTEIAEHLAELGVHIIVGHHPHVVQPVTMVGDTLVIYSLGNFLSAQ
                     KELFKLVGLLVSLDVVKTDTPEGVKISLENVTGTLLYNPRHSPLGRYVVVPFDMMTEA
                     ILKDFDKVHSRFSAYVKKDDTIAIEPPADAHEQECVDCGS"
     gene            complement(9166..>10071)
                     /locus_tag="PLM83_02490"
     CDS             complement(9166..>10071)
                     /locus_tag="PLM83_02490"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOK70310.1"
                     /translation="RTVIAHVVSYRFAEQIPTRGDLARGVGGPLMALWIIGVTIGVAG
                     VAIAYAKARSMALRSVSVRREELDAMVARICRNMKISCLPELVVAGWASVPMTLRRRS
                     RHVVVIPLKFLAPERQSDLELALAHELAHIKRHDLNWNWLPTAAKILFFFNPLIWVVD
                     RELGIAQEMACDEMAVRATRSKAARYGRVLLTMVRPDREAGSTWRVATASAGASAEVI
                     RRRLVELGHMQDPPARRVMASIVTLLFMCLLVMPFQIVEAPRDGLPRPGVSRIRDKIY
                     ISEPWGEKASLDGVTIGIDFEPSGK"
BASE COUNT         2328 a         2973 c         2398 g         2372 t
ORIGIN      
        1 cccatgaccg atggcctagt tccggcggaa gttcacacct ccggatcgtt tctgatgact
       61 atcggttccc catgcccaga tcttcaaccc ggcacgtatt ggccagcgtg taccagagct
      121 cccacacacc ataccagttt gcaatatagc tattgcgagc ttttttcaaa ttgagctgcg
      181 ctcgctccag gtccactaac agcaattcgc cgtcttcata cgacttcaaa gctgtatcat
      241 aggactcttg ggattgctca agctgcttca cccatttggc tgccgaccat accgaaacat
      301 gcaattgctc aagacttctc tccacattct ctgctaatgc ctcacgcctt tcagttaccg
      361 actgcctctt ttgatccaca ttgatctctg ccttctgtgc ttcgagagag cgcgaaagcg
      421 agatgctcca tgtggccgac gctacaaact tgggaacgct gctccctgac ggaaattgaa
      481 cctctgcaga caccgtagga agcagacttt tgattgcttc aagttcccgg atggacgaat
      541 ctaggcccaa ttctgcctga gcaagctcga catcgctctc gaacgctgcc tcgatcatca
      601 catcaagcga aggcatgtcc aacgaaaact gcggaagatc ttccagctct gcctctaaca
      661 tgtcgacacc ggccagcttg ctcagttctc tcctagccca ccgctcctcg ttttccgcct
      721 tcaccagagc gatttcagca tcgagtacag aaagctctgc ttgctccacg tctctttccg
      781 tagagagccc aacctggcat cttttcgttg ctatctcaag tgcagtctgg gcaagccgcc
      841 actcctcttc agccgtctcc cgattcttct gggacttcag cgcgccaata taggctttct
      901 gcgccttgag ctttattgac gactctgatg catccagctt catccttgcc atatccgtcg
      961 ataccttggc gtcctcactg gctcccgaat ggccaaacag tcgctgagcg ataccttcat
     1021 ccgaacccgc aagccaagtg tgtggaaggg tatatgatag cttgagagaa cccgaagact
     1081 tgttcaaatc gtaagctgcg ttcaagctga tcctgtcagt tatcgggagt ttggcggaaa
     1141 ccttctcagc atcgccgcgg tctcctgtgg attcagttaa ctgactagcg ctcgacattc
     1201 cgctggagat ggttatggat gctgaacgaa cgggcacgct cagtccctcg ccaatcagcg
     1261 caagttccag cggctgtcga gcttgcggac tgttcttcca gacgttgaca gcagcatccg
     1321 caaacgtgtc gattgaccag cgtgccccga atgtcccgtc agccgttgcc ccagaagagg
     1381 ccgccagaat cagcatgatg atgaaaatcg gcattcttct gccaacgcta gccctcatgc
     1441 gccatccctc acattcctta tcattcgtat ctcaacccca cgatgggatc gagctttgcc
     1501 cccttgtatg cagggtatgc cccaaagaac actcccacta tcaatgacag agtaaatccc
     1561 aacgccactg atttcatgtc caccgcagtg ggccagctta acacttggct cacgatgcgg
     1621 gacccgacca aaccgacaac gattccaaga aggcccccag caagactcaa agcagcggcc
     1681 tccaacatga actgggacaa gatctcatgc tcctttgctc caatagcctt gcgtatgcct
     1741 atctcgcgag tccgttcagt cactgaaact agcattatgt tcatgatccc gatacccccc
     1801 acgaccagcg acaccgctgc aatgcctgac aacagcaatg tcatggtgtc agtcacatca
     1861 gatgctactt ccagcatgtc ggcttggtta gatatcctga atgagtcttc gtcgccggta
     1921 gccatcatga gcatggcgta tatctgactg taggcaagat ccattgatga agcgtctact
     1981 gcttgggcaa tgattgtgtt tatgttcttg ctcccggtta tacgacgctg catcgtcgta
     2041 tacggcacat acatggcgtt atccgaactt gcgaatccgc tgtcgccctg gcgttgcgcg
     2101 acgcctatca ccgtgaaacg cactctgtca agccgaactt tctgacccag gggattatca
     2161 tctccgaaga gttcttccgc cagatccgcc ccgagaacgc aattccatga acctttatcg
     2221 atatccgcat cagtcagaaa agagccttgc gctaacttca ggttccttac cgaaggatag
     2281 tttgctgagg tgccagttac ggttgtgctg agaccttctg ctccgtgcac tactgtgctc
     2341 tgcctttgca cctgtggcac tacctcgcca acggtgttga tgttttcaat gatcggcaga
     2401 atatcggagg taataacaga tctcaagctt ctatatccgc tgcttatggt tatcagattc
     2461 gaacctagtc tctgtatttg cgactgcgtc tgcaccttag ccccagtgcc tatcgatacg
     2521 gtgatgatca cagccgatac accgattatc acgccgagag ccgtcagtat tgaacgaaac
     2581 ggattacttg taaccccgcc caaggcgagt ctcagatagt cggcaggcct cacttgtcta
     2641 cacccccctc caaagcgcga acatcctcaa tgcactcgcc gtccctgaac gtcagcactc
     2701 taggggtata ggctgcaatg tcaggatcat gggtaacgat taccacagtt atcccccgct
     2761 tgttcatgtc ggcaaggatg tccatgatct cgtagctcga tttggtgtcc agatttccag
     2821 tgggttcgtc tgccagaact acgcggggat tgcttaccag ggcacgggcg atggcgaccc
     2881 tctgctgttg gcctccagaa agctcatttg gtctgtggaa tgccctgtcc gcaaggccca
     2941 ccatgtccaa ggcgcggatg gcttcttccc gtctgatgcg aggatggact cccgcatata
     3001 ccaacggcag ctccacattg cctacggcag tcagtttggg cagcaagtta aaggactgaa
     3061 agacaaaacc catgatcttg tttctaactt ctgccagctc gtctgagccg aggctcccaa
     3121 ctaagcagcc atccagccag tattcgccag aggtgggctt gtcaaggcat ccaactatgt
     3181 tcagcagggt agacttgccc gatcccgact ggcccatgat cgacacgaac tcgccttcgt
     3241 ccactgccaa gtcgactgct ttcaatgcac gaacctctat agatcccttt cccatggagt
     3301 aggttttgct gacttccttc aattcgatga gactcattgc catcgcccct atctctgagg
     3361 tggagcgaat ccgccgctcg gaccgcccat gccagggact ccggtcggcg cctcgccggg
     3421 catcccaggc atcatccccg gcatgccttg ctctggttca cgtcctgcag agccctgatc
     3481 ctcatttgat gatacagccc taggatttgg aactgccacc atgtctccga gattcagacc
     3541 gtccacaatc tcagttccgc catcagttgc gagaccgacg ataacagggc gcaactgtac
     3601 actgccatca gcattgtaga tgcgggccac agtctcgcct ctgcgcgttt ccaaaacact
     3661 gttggggacg atgagcgcgt tctcgactcg atccaccatg atgctggcct cagcgctcat
     3721 ccccgggcgt acatcttgag tggggttgtc cagtctgatg tctacattga atacaaccac
     3781 gccggactgg ttttgaccga tttttcccac aaaacacacg ttgccagcat atttccgatc
     3841 gtccaatgcg tccatgacaa cctcggcgcc ttgccctacc tcgacagagc ctatgtcgta
     3901 ctcatcaacg gtcactctga ccactacatc gctgaggtct gtaattacgg cgacaggatt
     3961 ggatgtagac acgcccgcgc cgactgtcgc cttcacgctg cttaccaccc cactgatcgg
     4021 ggcccggatc acactgtcgc tggctttggt aacaaggcga tccagcttta gcgccgccgc
     4081 attataggac tctttggcct gcagcacatc tgactcggct gacctgagtt ccttctcagt
     4141 cgccgcccct tttgcatgga gactccggat tttctcgagg gcaacttccg aagacgccaa
     4201 gttctcccgc ttctgcctca cttcagcctc agtctgctgc acctccagtt catcgcgttc
     4261 caacctgatc aacacgtccc ccgctctaac ctcggttccc tcgtccacat ccacctgggc
     4321 gacgatgccc gccttggttg gatatacgtc gacagtccga atggtctgca cattgcctgt
     4381 ggcatagatg tcagttgaga gctctccccg tctgacctcg ataggtatgt atgggttgct
     4441 cgcggctcct ggcttcgacc tggagcgatt ccaccacatg aatccggcac cgcccgccac
     4501 ggcaatcagc aacacaatga tcacagcagt tttgcttcgt ttcatgcctt cgacctccat
     4561 cactcaccgc ctgagcacag attaccagcg tcatgtgtcg ggaatgtgaa cacatgatga
     4621 acttttgcct tctcttccag cctctagggt tcccggacgg cttcgaagag acactgaaca
     4681 cttctgagtg ccaaatgtcc gaaagcagaa aatgggcgat gtgaatgtga agatattgga
     4741 gggataagta aaaacgtgcc tcagggaaga ctggatagtg caacgcatca gcgctcttca
     4801 tgtaatcttg atctgtggtg ggtcaggatt gtggccctta ggcgggttta ggaagcctgc
     4861 gcaccagctt tgtagaacaa ccaattacag tgtgcggaaa ggaggcgatt gggctagatc
     4921 ggattcaaac taagaatagc actagacccc gtttcatgga tgcatacctg tgacctgtga
     4981 atttcccgac acggttttgt catagcgcca gtgattacca tattgctgta tggccagtca
     5041 aagagcgtga tggaggggtt cttagctgca atctcaatca tgtgatgtgc gagcctgaag
     5101 cggggattgt cgataagtgt ctaagccctg accaaatggc ccgtactgag tcatctatcg
     5161 cctagaacgt gaagcgggag tttccgttgg acggacgata atcctcaatc agcaaagtga
     5221 atccatgatg ccccaaatta cgatctggcg tccacacacc ttctcatttt gcttggtgtg
     5281 ttgtacgcgt atacgaaggc tccctgttaa ctgcatacgc actgtttcgg gcacagtatt
     5341 ctcggggccc ggcccacatg ttctttttcg ggttcagctc tggagaatac gaatgtccca
     5401 actgtttttg ctgtattcat gaccctttga actacggttt gtaaccgcat cgtctatatc
     5461 cttcggtgaa cggacttctg ctgccgcttc tgtgatccgt agcagcctcc atgacttgct
     5521 tgttccgcca atctacgtat cttcagacaa agatactgga acatccaagc atctaggctt
     5581 tcgtaacctt aagctgtcag ccatcatatt agctaacttg ggctatggaa gctgaaaact
     5641 gttcactgtc gggttgctca gctataacat ccccgtggga agccaagcat gcctgattgc
     5701 cgtgatagac aactctcccg gcacaatcca gttatcactc gatgaatacc gctgaatacc
     5761 agttgggcgt ttcatcctcg ctcttgagag tatccaacgg caggccgaag cggtgcacgg
     5821 tcttgaaaac gtcaatgccc acactgtgaa aggccggacg cgccttttgg ggattggcgc
     5881 acggcttacc gacaaatccg gcgcattgcc cgcatatggc gcaatcgcta agtgaaaagg
     5941 ccagataata tccgcgcaaa aaggcttcgc gttcaatgcc gaaggagaca tcctgcgaaa
     6001 tacgtttatc cgtagaatga atgataatcc cccaagaata acggcgcaac acctgttcat
     6061 actcccaagg cctaagagaa agaggccgag aggggcaagt gtgcccatag ccccagtcac
     6121 cgcaaccaaa agcgcacttt aagatagtgc gcggatcgaa tacgatatca ctgatttcaa
     6181 aggggacggc atgatcagct ccccactcca aggccagatc cacgaaatac tcggcatcca
     6241 taagccacta cacctctttg gaagtctcaa cttggtccgt ctccaagata ggattcctca
     6301 acttaatccc gtaaacaatg ccacggcctc ctcaggcaaa aacaaatccg acgctaaccg
     6361 tcgggctcgt ttgagacaag ccggggcact catcctattg ccacagtatc cgtaaagcct
     6421 cttccagctc gacattttct ccgcttcatt ctcatctagc gacttcccgt acggatggct
     6481 tgccacctag gagatcccga attcccatcg ccaacgctca cgcgcttcga gttgatgagc
     6541 gcaatgacag gtccgcacgg caagttagtt gtaatatctt tcctctggtc cgaaacagga
     6601 cgtattgcca ccgatggaaa aggccactgc aatcacgata gataccgcaa gtaacatccc
     6661 cctcggactg aaatccgagg attccctgga catgccccag gctcgttggc agctgccttg
     6721 tctggaatgc atttgtgatc cacagaccgt tttcgtccct ctgatatggc agacagcatc
     6781 aagattaatc aaaccataga catgcgaata gcttctgcca cagttgtccc catcctccca
     6841 ccggatttcc gcttcaagct tgtatttatc aataaatcct gtagttcgga tcttgtaaga
     6901 tgctgctcga ccaggctagg gctcggcgca aagcttatcg atcttgggat cacggatcca
     6961 ggttgcatgg aattgcatca tgatagtcct tatgactcat ttagtttctc attccagcgt
     7021 tttccggcct gcccttgtag aaaaactcgc catgtcctca aaaacatgtc cacagaatag
     7081 cagatgggcg ccgcctcacc gacggcaact ccgaaggttc tactttcgag ccaaagctac
     7141 tttatcgcga agacagcccg gatgttcagc ctatgagaac actgtttggc agtagattgc
     7201 gcgatacgaa gcacaggcgg cttttgaaag ctgtagtcac agctaacagg ctctatgggt
     7261 tgatggatac gctgatgagc gcttttctca acaacctgac tccgtctgaa attcagacct
     7321 tggccgctta ggaatgctca gaatacccca gtgcctcggg catgaagccc tgctgatgtt
     7381 atgattatac caaactcaag gaggttagag gagaaagctg tgtcaggatc ccccatcatg
     7441 acaacacccg gctaacgcga agaggagtat gtcctgagcg gggcgaattg agctattgat
     7501 tgaaacgcat gctcatggag gagatcatga tccgaagaaa gacctttccg ctcgtcctta
     7561 tcagcctttc cctcattctc gttctctggg cacagcccgg ggccccggga atgtcttccc
     7621 gtttctcctt tgctgctgtt ggagacgccc ttatccacca aggtgtgtac aacgccgcca
     7681 gagtagagga cgacgccttc gattttcgcc ctatgctccg cctcatcgcc gaccgcattg
     7741 cccgccatga cctcgccttc tacaaccagg agacaatcct aggcggcgtc gagctcggtc
     7801 tgtcgacgta cccacgcttc aactcccctc aggaggtagg ggacgccttt atcgacaccg
     7861 ggttcaacct cgtatcgtta gcgaacaacc acaccctgga ccgaggggaa aatgctgttc
     7921 tgtgctctct ggagtattgg agcggtaaag aaagcgtcct cacagccgga agctacttta
     7981 gccagacgga tcggaacgaa atacagacgg tggaggtaaa tggcatcacg tgtgcgttcc
     8041 ttgcctatac caccctcacc aacggcctga gaccccccaa tggcaaggca tactacgtga
     8101 acgtctacga ccccgagaca gccagggccg atgtggagaa agccaaggcc aatgctgatg
     8161 tggtcatcgt ctccatgcac tggggtgatg aatacgtttt cgcacctact aaggagcaga
     8221 cggagatagc ggagcacctc gcggagctcg gcgtccacat aatagtagga caccaccctc
     8281 acgtagtgca gccggtaacc atggtaggcg atactctggt catctactcc ctgggcaact
     8341 ttctctccgc ccaaaaggag ctgtttaaac tggtcggcct tcttgtctcg ctcgacgtag
     8401 tcaagacgga cacgcccgag ggcgtgaaga tcagtctgga aaacgtcacc gggaccctcc
     8461 tatacaatcc ccgtcactcc cctctagggc gctacgtggt agttcctttc gacatgatga
     8521 ctgaagcgat cctcaaggat ttcgataaag tccacagcag attctcagca tacgtcaaaa
     8581 aggacgacac catcgccatc gagccccctg ccgacgcgca tgaacaggag tgtgtagact
     8641 gcggttcatg agacagcctc tggacgttca ataccttggt gcgggctcac atacgagctt
     8701 caactcgcac gccaagaccc tggaccacaa cgtcaaggcc gtccgtttgg cagtctatag
     8761 cgccctgcag gcctccctac cttcacttcc tgattcttta gggaataccg attcttccta
     8821 aagaccgtgc ctcttgcacc atgcggctct gcgaacacgc gtgtcctcgc aacagaagcc
     8881 gtggcgatgg ttatgagtaa ccaaatattg gaatgcagat gagcaagagg cccgactagg
     8941 ctgcgaagat acaagttgct cgctaccctg catactacca aaactacatg ctcagcgcga
     9001 ttttatcgac acagtctgaa gcatctgtcg gcgaacggtt caaaacccaa tcgtccgcaa
     9061 aaatgcccga agttggccag tggatctctt atggaagtgt tgagggcaac cggtagcgtc
     9121 agtcggctgc gacttgatgg ccatggcaat gtcttctttg aaccgtcatt tgcctgaagg
     9181 ttcaaagtcg atgcctatag tcactccatc gagggatgct ttttcgcccc aaggctcgga
     9241 tatgtatatc ttgtctctga tcctcgacac tccaggacga ggcaggccat cgcgtggagc
     9301 ctctacaatc tgaaatggca tcaccagcag acacatgaag agcaacgtca ctatagatgc
     9361 catcactctt cttgcaggcg ggtcctgcat gtgacctagc tcgaccaggc gtctccgtat
     9421 tacttcggca gacgcccctg cagacgccgt cgctactcgc catgtggagc ctgcctcccg
     9481 atccggtctg accatcgtga ggagaaccct gccgtacctc gcagctttag accgagtagc
     9541 gcgcacagcc atctcgtcgc aagccatttc ctgagcaata ccgagctcac gatcgaccac
     9601 ccagatcaga gggttaaaga agaatagaat cttggccgcg gtaggaagcc agttccagtt
     9661 caagtcgtgc ctctttatgt gggccaactc atgggccaac gccaactcca ggtcgctctg
     9721 acgttcggga gccaagaact ttagagggat aaccacaaca tgcctgctcc tccttcgcaa
     9781 tgtcatcggg acagatgccc agcctgcgac tacaagctcg ggcaggcagc tgattttcat
     9841 gtttcggcat atccgagcca ccatggcatc gagctcttcc cgacgtactg acacgctgcg
     9901 caacgccatg gagcgcgcct ttgcataagc tatcgcgacc cctgccacgc ctatggtgac
     9961 cccaataatc caaagagcca tcaaaggccc gccaaccccc cgggccagat ctccgcgggt
    10021 gggtatttgc tcagcaaaac gatatgacac gacatgtgca attacggttc g
//
LOCUS       DAOSAI010000037         9990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4435_length_9990_cov_3.471163, whole genome shotgun sequence.
ACCESSION   DAOSAI010000037 DAOSAI010000000
VERSION     DAOSAI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4435_length_9990_cov_3.471163"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLM83_02495"
     CDS             complement(<1..832)
                     /locus_tag="PLM83_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HOK70311.1"
                     /translation="MTTKRVGGSTIVYSTPPVIVSAATVAGPKESEGQMAQWFDELVP
                     DDMFGERTPEKAERRFMEDAIKIALGSARVLPEQVNMIFAGDLLNQIITSSFTAEGFG
                     IPFVGLYGACSTMAQSLTMASAMVDGGYADLALAVTSSHYQTAERQFRYPIELNIMRK
                     STSQYTVTGAGAALVGRSGGKVKVTEATIGKVIDMGIKDPNQMGPAMAPAAADTIFAH
                     LADTGRDPGYYDIILTGDLGRVGAEILRQLMAEKGVPLGDNYSDAGILMFEGVRGTGV
                     G"
     gene            complement(836..1303)
                     /gene="spoVAC"
                     /locus_tag="PLM83_02500"
     CDS             complement(836..1303)
                     /gene="spoVAC"
                     /locus_tag="PLM83_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HOK70312.1"
                     /translation="MASPGAKKQKADERAKYQRLAASIVPKPPLVRNMVVAFVVGGLI
                     CTVGQGVINAFMSRGMTQDEATAPTLATMILIGSVLTGLSAYHHIGEFAGAGAAIPIT
                     GFANTIVAAAMDFKREGYILGMGAKMFIIAGPVLVYGILSGVLVALIRWLYRG"
     gene            complement(1349..2059)
                     /locus_tag="PLM83_02505"
     CDS             complement(1349..2059)
                     /locus_tag="PLM83_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK70313.1"
                     /translation="MMWNADTQGGSAFLSSREAEARRHAGLVMSLARRFAGRAEIADL
                     YQAGCVGLLRAIDRYDQSRGTTFSTFAVPIILSEIVAYLRGSGPIHVPRPVRELAQKC
                     RHAQDSLRAELSREPQATEIAARLGIDAADVVYAMESEAHVYSLFDQVSFDHESLELH
                     EVLPGDLGVETSVCESIALSDALSALSEIERTVVAMRFFEERTQAETASLLGISQPQV
                     HRIEKAALLALRRSMEGE"
     gene            complement(2056..2493)
                     /gene="spoIIAB"
                     /locus_tag="PLM83_02510"
     CDS             complement(2056..2493)
                     /gene="spoIIAB"
                     /locus_tag="PLM83_02510"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HOK70314.1"
                     /translation="MHAKVNHAKVEFDAIPGNLGFARVAAASFAAGLGFTVEDLEDIK
                     LAVSEAVSNCIIHAYPGPGEARPTVLMVMEARESEFVVSIEDKGIGIADVAMARKPGV
                     TTDSERLGMGFTLMEALADQVDVKSELGVGTRVEMIFSPERGG"
     gene            complement(2478..2840)
                     /locus_tag="PLM83_02515"
     CDS             complement(2478..2840)
                     /locus_tag="PLM83_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HOK70315.1"
                     /translation="MIEWEVSGNSLVVEIAGELDLTCADEFRRVLDSLIDSHPGVARV
                     ILCMDRVAFIDSSGIGVLFGRHRRMALRGGTLAIASPSPQAMRALDLLGVPSVIHVYD
                     TLKQALAAEAEVRKCTRK"
     gene            complement(2935..4149)
                     /locus_tag="PLM83_02520"
     CDS             complement(2935..4149)
                     /locus_tag="PLM83_02520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOK70316.1"
                     /translation="MSYIRCGRACRAALALVALLLVVKVGICGAQSAPLAAMSLDLDK
                     KITAPSAILMEASSGRVLWEKNADVARPIASVVKVMTLTLVMEALEAGRIDLDDIYIT
                     SEHAASMGGSQIWLEPGESMAVSELVKAVAIESANDAAVALAEHVAGSEPAFVALMNE
                     KAQALGCKNTHFINATGLPGKSPSEDCVSSARDVAIMSRELLKHPRIHEWLTIWIGYV
                     RDGQNMLTNTNRLIRFYPGADGVKTGYTERSRYCLSATAVRNNVRVIGVVLGVPTSAV
                     RFSEASTLLDYGFKMCEAHVIARSGQEMGRVRVLRGIDTEVGVTVDSDFTVVTLRGEG
                     DGVDARLELPQYVSAPVERGQRVGKIVAYVEGVEINSADLVAVDDIPKAGAFTLVVRS
                     TREFVRSLFDFR"
     gene            complement(4224..5531)
                     /locus_tag="PLM83_02525"
     CDS             complement(4224..5531)
                     /locus_tag="PLM83_02525"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOK70317.1"
                     /translation="MRVPDIIMKKRDGGELSPEEIFFLIDGMVSGDVPDYQMSAFCMA
                     VYFQGMTPAETTALTLAMARSGEIVDLSAIRGTKVDKHSTGGVGDTTTLVLAPMVAAT
                     GVPVAKMSGRGLGHTGGTLDKLESIPGMRVSLSEEEFIEQVNRIGIAIVGQTEDVAPA
                     DKILYALRDVTATVESIPLIAASIMSKKLATGADAIVLDVKTGTGAFMHDLSQAEALA
                     GLMVDIGRMARRKMAAVISDMNQPLGHGVGNTLEVIEAVQTLAGEERGRLFDLCLELG
                     ARMVVMGGAESSIEAARARLNDVVDSGAALEKLREMVVAQGGDPAYIDDPRRLPVAPY
                     SEYITAPCSGYVASVDAMTLGKAAMSMGAGRARKEDSVDLECGLVTLVSIGDPVEQGQ
                     PVAKLYASSTSLLTEGLSVAAKAYTYSAEPIEPPPLVYRTIES"
     gene            complement(5561..6757)
                     /locus_tag="PLM83_02530"
     CDS             complement(5561..6757)
                     /locus_tag="PLM83_02530"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOK70318.1"
                     /translation="MSMRALIIVFDGVGIGELPDAGEYGDLGSNTLVNTSRAVGGLNL
                     PNLEKLGLGNLAAAAGHEICGVAAIEHPLASYGTMAEKSPGKDTTTGHWELAGIQLDQ
                     AFPTYPKGFPPEIILPFQEAIGRKVIGNIPASGTKIIEELGQEHIRTGSPIVYTSADS
                     VFQIAAHEEVIPPAELYEMCKIARELLSGPHAVGRVIARPFIGVPGNFIRTERRRDFS
                     LVPPGPTLLDLVAREGLEVMACGKIDDIFANQGITRSKHVAGNDAIFDAVLEFLDSGH
                     SGLIFANLIDFDMKWGHRNDVEGFARGLEHIDSRLPELLERLGESDLLAICADHGNDP
                     TTPSTDHSREYVPVLAYSPARQRKGRCGAHVGVRETFSDLGATVADFLEVGWTLRGES
                     FLNETL"
     gene            complement(6754..7680)
                     /gene="xerD"
                     /locus_tag="PLM83_02535"
     CDS             complement(6754..7680)
                     /gene="xerD"
                     /locus_tag="PLM83_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOK70319.1"
                     /translation="MLPSVEEYINYLAVERGLSENTLEAYARDLSQFAEYAAKSGRQS
                     DEPIDDDILTASQSMVASFIARSIESGGSPSSAARRLTSLRRYYRFMLQEGYVKADPT
                     ANLESPRQPQRLPDVLTEEEVNRLLSSAPGYDPQSLRDTAMLELMYASGLRVSELISL
                     TTEDVDMELGYVRCVGKGDRERIVPVGRRAIEAIERYLAFGRRELAGVCKDNYLFITN
                     RGTAMTRQGFWKMIKRRALKVGISTDITPHTLRHSFATHMLENGADLRSVQELLGHAD
                     IRTTQVYTHLTQVRLREVYNKHHPRALGEGDA"
     gene            complement(7783..8004)
                     /locus_tag="PLM83_02540"
     CDS             complement(7783..8004)
                     /locus_tag="PLM83_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70320.1"
                     /translation="MDPESGGPRIIGLRLFRKAYYKGGDFRIVLEKTWTVRLFASGSM
                     QDYTDQRRRDRLEIGRYGYADTGGSYYPA"
     gene            complement(8260..8895)
                     /gene="spoIIM"
                     /locus_tag="PLM83_02545"
     CDS             complement(8260..8895)
                     /gene="spoIIM"
                     /locus_tag="PLM83_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOK70321.1"
                     /translation="MARARRTSRSVLSHARSNMVGYWLTFVVFITGVSCGAVQASMLK
                     EGRTELFSYLEAGLASLRHTLPVAPGKALAYSLGSNLASVGLIWLSGLTILGFPGALV
                     ILFVRGFSIGFTVAFMVRELSGPGAALAVASVLPHNILAVPALILACFVSMSFSWAVI
                     GTNLIGMASDVKLAFSRSVAGVAISAILLSAASFVQAYVSPAILILAARFV"
     gene            complement(8959..>9990)
                     /locus_tag="PLM83_02550"
     CDS             complement(8959..>9990)
                     /locus_tag="PLM83_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOK70322.1"
                     /translation="ASTRVLKDIEDLLESGDMTEAPGGGFRYLDKTTVIPGAEVESAC
                     PGSARGAHYLSLFPTLTALRQYSRSLSRYVSNMDLSTQRARLSPVELAQLAADSGGLF
                     LVAHAFTPHKSLYGTCARRMSEVFGEDVRKLVDGVELGLSADTDMADRIGELSHYTFT
                     SNSDAHSLPKIGREYNVMRMKAPSFSEVRLALARLDGRRVVANYGLDPRLGKYHLTAC
                     EECGHRFKAWPNSCACPVCGSVRTVRGVADRIDELSDTAEPIHPPHRPSYNYQIPLEF
                     VPGLGPARIGALIDAFGTEMTVIHETIESDIAEVVGEEIAHRICLAREGRLGIQSGGG
                     GAYGKAVVE"
BASE COUNT         2244 a         2985 c         2546 g         2215 t
ORIGIN      
        1 cccctacccc agtacctctt acaccctcga acatgaggat gcctgcatct gagtaattgt
       61 ccccgagggg cacccctttc tccgccatca gttggcgaag tatctccgct cccacgcgcc
      121 ccaaatctcc ggtaaggatg atatcgtagt atccggggtc ccgtccggta tcggcaaggt
      181 gtgcgaagat agtgtcggcc gcggcaggag ccatagcggg ccccatctgg ttggggtcct
      241 ttatgcccat gtcgatcact tttccgatag tcgcctcagt gaccttgact ttgcctcctg
      301 atctgcccac caatgcggcg cctgccccgg tcaccgtata ctgcgaagtg ctctttcgca
      361 taatgttcag ctctatcgga tagcgaaatt gcctttcagc ggtttggtaa tggctgcttg
      421 tgaccgcaag ggcaaggtcc gcataacccc cgtccaccat tgcggaagcc atggttaagc
      481 tctgcgccat agttgagcaa gccccataca gcccaacgaa aggaatgcca aaaccctctg
      541 cggtgaagct tgaggtgatt atctgattga gcagatcgcc ggcgaatatc atattgactt
      601 gttcgggaag gacacgggca ctgcccaggg cgatcttaat ggcatcttcc atgaatcgcc
      661 gttccgcttt ctcgggcgta cgctccccga acatgtcatc gggcaccagc tcgtcgaacc
      721 actgagccat ctggccctcg ctttcctttg ggccggcaac tgtagcagcg ctaacaatta
      781 cagggggagt ggaatagact attgtgcttc cgcctacacg ttttgtcgtc atagcttacc
      841 ccctgtagag ccacctgatg agcgcaacca gcacgccgga aagaatcccg tatacgagga
      901 ctgggcctgc aatgatgaac atcttggcgc ccatgcccaa tatgtagccc tcacgcttga
      961 agtccatggc tgccgccaca attgtgttgg caaatccggt aatgggaatg gccgctccag
     1021 cacccgcgaa ttcgcctata tggtgatatg cagaaaggcc tgtcagtacc gagcctatca
     1081 gaatcatagt ggccagcgtg ggtgcggttg cctcgtcctg agtcattcca cgggacatga
     1141 aagcattgat cacaccctgt cctactgtgc agatgaggcc tcccacaacg aatgccacca
     1201 ccatgtttcg gacaagaggg ggcttgggaa cgatagatgc cgcgagcctc tggtactttg
     1261 ctctttcgtc agctttctgt ttctttgctc ccggacttgc caagatgcac acccccacat
     1321 gggctagtat gcaaccgggc cggaggtttc attcaccttc catgcttcgc ctcaatgcaa
     1381 ggagggcggc tttttcgatc ctgtgcacct ggggctggga aattcccagc aagctggctg
     1441 tctcagcctg agtgcgttct tcgaagaagc gcatcgccac caccgtgcgc tcgatttctg
     1501 acaaggcgga aagagcatcg gagagggcaa tggattcaca caccgatgtt tcaacgccca
     1561 aatcgcctgg aaggacctcg tgcaattcga gggattcatg atcgaagctt acctggtcga
     1621 aaagggagta gacatgtgcc tcagattcca tggcatagac gacatccgct gcgtctatcc
     1681 ccagacgagc ggcgatctct gttgcctgag gctccctgga aagttcggcc ctgagagaat
     1741 cctgtgcatg gcgacatttc tgcgcgagct cacgcacagg ccggggaacg tggatgggac
     1801 ctgagcctct caggtaggcc actatttcgg agagtatgat aggcacagca aaagtgctga
     1861 aagtcgtgcc tcgcgactgg tcgtaccggt cgattgcgcg gagcagacca acacatcctg
     1921 cttggtagag atctgcaatc tccgcccgac ccgcgaatct tcgtgccaac gacatcacaa
     1981 gtcccgcgtg tcgccgggct tcggcttcgc gggatgacaa gaatgcagat ccgccttgcg
     2041 tatcggcgtt ccacatcatc ctccccgctc cggagagaag atcatctcaa cgcgcgtgcc
     2101 tacgcccagt tccgacttta cgtcgacctg atcagccaag gcttccatca aggtaaatcc
     2161 catgccaagt ctctcggagt cagtagtcac gccaggcttt cgcgccattg caacatcagc
     2221 tatgcctatt cccttgtctt caatggagac cacaaactct gattcacgtg cctccatgac
     2281 catcaatact gtcggccttg cttcgccagg accggggtaa gcatggataa tgcaatttga
     2341 cactgcctct gagacggcaa gcttgatgtc ttcaaggtct tctacagtaa aaccgagtcc
     2401 agcagcaaag ctggcggcgg caacgcgggc aaagccgaga ttgccaggga tcgcatcaaa
     2461 ctcaaccttt gcatggttca ctttcgcgtg cattttctga cctccgcttc cgccgcgagg
     2521 gcctgcttca gtgtgtcata gacatgtata accgaaggca cacccagaag gtccaaagcc
     2581 cgcatcgcct gcgggctcgg agaggctatc gcgagcgttc ccccgcgcaa tgccattcgc
     2641 ctgtgcctgc cgaacaacac ccctatgcca gaactgtcaa taaacgcaac tcggtccatg
     2701 cacagtatga cccgggcaac acctggatga gagtctatta atgaatcgag aaccctgcga
     2761 aactcgtcag cgcaagtcag gtcgagctcg ccggcgatct ctacgacaag actgttgcca
     2821 gaaacctccc attcgatcaa ccgccatccc ccgcctcccc attggcttga ctcagagatt
     2881 ctctgccaaa gcggcttctc cttccagcta atggccgaca agggcacgac agaatcagcg
     2941 aaaatcaaag agagacctga cgaattccct cgtcgacctt acaaccaagg taaacgcgcc
     3001 tgctttcgga atgtcatcga ccgccactag atcagccgag tttatctcca caccttcaac
     3061 ataggccaca atcttgccaa cacgctgccc ccgttcaacc ggagcgctaa catattgggg
     3121 aagctccagc ctggcatcga ccccatcgcc ttctcctctt aaagtcacca ctgtgaaatc
     3181 tgaatcgacc gtgacgccga cttcggtatc gattcctcgc aggacgcgga cccgccccat
     3241 ttcctgcccg gagcgggcga tcacgtgagc ttcgcacatc ttgaaaccgt aatcgagcaa
     3301 cgtagaggct tcagagaacc tgacagcgct ggttggaaca ccgagcacta cccctatgac
     3361 tctgacattg tttctcacag ccgtagccga gaggcaatac ctagatcttt cggtatatcc
     3421 agtcttcact ccgtccgcgc ccgggtagaa tcgtatcagc ctattggtgt tggtcaacat
     3481 gttctgaccg tccctgacgt agccgatcca tattgtcagc cactcatgaa tacgcgggtg
     3541 cttgaggagt tcgcgggaca ttatcgccac atcccgcgcg ctggagacac agtcttcact
     3601 tgggctcttg ccgggaagcc ctgttgcgtt aataaaatgc gtattcttgc atcccagagc
     3661 ctgcgccttc tcgttcatca aggccacaaa ggcgggctcg ctcccggcca cgtgttcagc
     3721 tagagcgact gcagcatcat tagccgattc tatggccacc gccttcacaa gttcagatac
     3781 agccatagac tctccaggtt caagccagat ctgggatcct cccatggagg ctgcgtgctc
     3841 ggaagtgatg tagatgtcgt ctaaatctat ccgacctgcc tcaagagcct ccatgacaag
     3901 ggtgagcgtc atcaccttta ccacgcttgc gatcggacgt gccacatcag cgttcttttc
     3961 ccacaagaca cgtccgctgc ttgcctccat cagtatggcg ctgggagccg taattttctt
     4021 gtcaagatcc agcgacatgg cagcaagagg ggcgctctgc gcgccgcaaa tccctacctt
     4081 gacgactaac aacagggcaa cgagagcaag tgccgcccta catgccctac cgcagcgaat
     4141 gtatgacaag aagcatgcct ccctccttca cttctctcaa aagagcatat tcggaggcat
     4201 gccctgttat acagttcccg cgttcaggac tctatggttc gatacacgag cggcggaggc
     4261 tcaataggtt cggcggaata agtgtaggct ttagccgcca ctgacagtcc ttccgtcaac
     4321 agagaagtgc tgctggcgta cagttttgca actggctgtc cttgttcaac aggatcgccg
     4381 attgagacca aagtcaccaa cccgcattcg agatctacgc tatcttcctt tcgcgcgcgg
     4441 cccgcgccca tggacatcgc tgcctttcca agggtcatcg cgtcgacaga ggccacatat
     4501 ccgctgcagg gagcggtgat atactctgag tagggggcaa caggcagacg ccttggatcg
     4561 tcaatatagg ctggatcgcc gccctgggcc actaccatct cgcgcagctt ctcgagggct
     4621 gctcctgaat caaccacatc gttcagtctc gcgcgcgctg cctctatgct cgattctgcg
     4681 ccgcccatca ctaccatcct tgccccaagc tccaaacaga gatcaaaaag gcgcccgcgc
     4741 tcttcgccgg caagagtctg aacagcttca attacctcaa gagtatttcc caccccatgg
     4801 ccaaggggct ggttcatgtc ggagatcacc gccgccatct tccgccgggc catccgcccg
     4861 atgtccacca taaggccagc gagtgcctct gcctgagaaa ggtcgtgcat gaacgcgccc
     4921 gttcctgtct tcacatcgag gactattgca tctgctcctg ttgccagttt tttactcata
     4981 atgctggcgg caatgagagg gatgctctcg accgtggcag taacatctct cagcgcgtaa
     5041 aggatcttgt cggcaggggc cacgtcctct gtttgaccaa ctatagcgat gccaatgcgg
     5101 ttgacctgct cgatgaactc ctcttctgat aaagacaccc gcattcccgg aatggactcg
     5161 agtttgtcca gggttccgcc tgtatggccc aaccctcttc cggacatctt cgccaccggc
     5221 actccggttg cagcaaccat gggagcgagc acgagtgtcg tggtgtcccc cacgccccct
     5281 gtgctgtgtt tatctacctt tgtcccgcga atagccgaaa gatccactat ttctcccgac
     5341 cttgccatgg ccagagtgag ggcagtggtc tcagcgggcg tcatgccctg aaaatagacg
     5401 gccatgcaga aagccgacat ctggtagtcg ggcacatccc cggaaaccat gccgtctatc
     5461 aagaaaaaga tctcttccgg gctcagttcg ccaccgtctc gctttttcat gattatatcc
     5521 gggactctca tttaaactcc tccgctcttg ccgcgcatag ctacagtgtt tcgttgagaa
     5581 agctctctcc cctcagtgtc catcctacct cgagaaaatc cgcgacagtt gcaccgagat
     5641 cagaaaaagt ctccctcacg cccacatggg cgccacatcg ccccttcctt tgcctggcag
     5701 gagaatatgc gagcacggga acgtattccc tggaatggtc tgtgcttggc gtagtggggt
     5761 cattgccatg atccgcacag atcgccagaa ggtccgattc accgagtctt tcaaggagtt
     5821 ccgggaggcg ggaatcgata tgttccaacc cgcgcgcaaa gccctccaca tcattgcggt
     5881 gtccccattt catgtcgaaa tctatgaggt tggcgaatat gaggcccgaa tgcccactgt
     5941 caaggaattc gaggacagca tcgaatatgg cgtcatttcc tgccacgtgc tttgaacgcg
     6001 taatcccttg gttcgcaaag atatcgtcta tcttgccgca cgccatcacc tctagcccct
     6061 cgcgggcaac gagatccaac aaagtgggcc ctggcggcac cagtgaaaaa tcgcgccttc
     6121 tttcggttcg gataaagttg ccgggcacac cgatgaacgg acgggcaatt accctgccca
     6181 cggcgtgagg ccccgaaagc aattccctgg cgatcttgca catctcatac agctcggccg
     6241 gtgggatcac ctcttcatga gcggcaatct gaaagactga atcggcggag gtatagacta
     6301 ttgggcttcc tgtgcggatg tgttcctggc ctagttcctc tatgatcttg gtgcccgatg
     6361 ccgggatatt tccgatgacc ttcctgccta tagcttcctg gaacgggaga atgatctcgg
     6421 gcggaaaccc tttgggatac gtggggaatg cctgatccag ctgtataccg gccaattccc
     6481 aatgtccggt cgtagtatcc tttccgggag acttttcggc cattgtgccg tacgaggcca
     6541 gaggatgctc gattgcggca actccgcaaa tctcatgtcc ggcagctgcc gcaaggttgc
     6601 ccaatcccag tttttcaagg ttcggaagat tcaaccctcc tactgcacgc gatgtattca
     6661 ccaaggtatt gcttcctaag tcgccatatt caccggcgtc gggaagctct ccgatcccta
     6721 caccgtcaaa cacgatgatc agtgctcgca tactcatgcg tctccttccc caagtgccct
     6781 cgggtggtgc ttattgtata cctccctcag tctcacctga gttaagtgag tataaacttg
     6841 cgtagtgcgg atatccgcgt gtcccagcaa ctcctggacc gacctcaagt ccgccccgtt
     6901 ttcaagcatg tgcgtggcga atgagtgcct gagcgtgtgg ggagtgatgt cagtggagat
     6961 gcccaccttg agcgcacggc gttttatcat cttccaaaac ccctgccgag tcatggcagt
     7021 tccgcggttc gttatgaaaa ggtagttatc cttgcagacg ccggcaagtt cgcggcgtcc
     7081 gaaagcaagg tatctctcta tggcctctat tgcgcgtctt cccactggaa cgattctctc
     7141 tcggtcaccc ttgcccacac agcgcacgta accaagctcc atgtctacat cttcagtggt
     7201 caaagagatc agctccgaga ctctcagccc agacgcgtac atcagttcca acatggccgt
     7261 gtcgcgaagg gactgaggat cataccccgg cgccgatgag agtaacctgt ttacctcctc
     7321 ctcggtgagc acgtcaggca gccgctgtgg ctgccgaggc gattccaggt tcgcagtggg
     7381 atccgccttc acgtatcctt cttgtagcat aaacctgtag tatctgcgta gtgatgtaag
     7441 tcgcctcgca gctgatgaag gtgatccgcc tgactctatg gagcgtgcaa tgaacgacgc
     7501 taccatggat tgactcgccg taaggatatc gtcatcaata ggctcatctg attgacgccc
     7561 ggatttcgcc gcatactccg caaactgcga gaggtcgcgg gcgtacgcct ctagcgtgtt
     7621 ttccgataga ccccgttcaa ccgctagata gttgatgtat tcttctacag atggaagcac
     7681 agaacactcc ctcctgttcg atctatagcc gctacagcag gagcggcaag ctatgccgcc
     7741 tgccaactca cgagctgatg cattaattcg gtgcgtcccc attcatgcag ggtaataact
     7801 tccgcccgta tctgcatatc catatcgtcc gatttctaaa cggtctcttc gacgttggtc
     7861 agtgtagtcc tgcatggatc ctgaggcaaa gagcctcaca gtccatgtct tttccaatac
     7921 aattctgaaa tccccaccct tgtagtaggc tttgcggaag agtcttagcc cgatgatgcg
     7981 cgggccgcca gactctggat ccacggatcc ccattctgca accacaaccc atatctaggc
     8041 cgaatagcgg cggcctcaga tccaaaatgt ctccttgttc tgcctcggtg gggcaaagac
     8101 acgagaaaac tggcgcatgg caacacatgc ggaaaaggag tgaacggatt gaggagggtt
     8161 cgccataaga agccaggtga agtgggccct cgaattcgcg gaacacgttg ttctgcgcac
     8221 tagccgctcg attaggcaaa aatacgtgtt gcgagttggt tagacgaatc ttgcagcaag
     8281 tatcaagatt gccggggata cataagcctg gacgaaagag gccgccgaca ggagaatagc
     8341 agagattgcc acccctgcaa ccgatcgaga gaaggcaagt ttcacatctg aagccatgcc
     8401 tataagattg gttccgatta ccgcccatga aaacgacatg gagacaaagc aggcgagtat
     8461 gagggctgga acagctagga tgttatgggg aagcactgat gccacagcaa gtgccgcgcc
     8521 cggccccgac aactcgcgca ccatgaaggc cacggtgaac ccgatggaaa aaccgcggac
     8581 gaaaagaata acaagcgccc cggggaagcc taggatcgtg aggcctgaaa gccatatgag
     8641 ccctaccgaa gccagattcg agcccagact atatgctaac gccttgccgg gcgcaacagg
     8701 aagtgtatgt ctcaggctgg ccaggccggc ctcaagataa gagaacagct cggttctgcc
     8761 ttccttcaac atcgaggctt gaactgcgcc acagcttact cctgtaatga atactacaaa
     8821 cgtcagccag tatccaacca tattcgaacg agcatggctc aacacagacc gggatgtgcg
     8881 acgtgctctg gccatcttgt acaccccctc catagagcat ggatatgcgc tacagggagg
     8941 atcaatgcca agatggatct attcgactac agcctttccg tatgcaccgc ctccaccaga
     9001 ttgaatgccg agacgccctt ccctggccaa gcatatcctg tgtgcaatct cctcccccac
     9061 tacctccgca atgtccgatt ctatagtctc atggataacg gtcatctctg tgccgaaggc
     9121 gtcgataagc gcccctattc tcgccggtcc caggcctggc acgaactcca ggggaatctg
     9181 gtaattgtac gaaggacgat ggggtggatg aatcggttcc gcagtatcgg aaagctcgtc
     9241 gattcggtcg gcaacacccc gaaccgtccg aacgcttcca cagacggggc aagcgcatga
     9301 atttggccat gccttgaatc gatgcccaca ctcttcgcac gccgtgagat gatatttgcc
     9361 aagacgcgga tcgaggccgt agttggccac gactctgcgc ccatcgagac gcgcaagggc
     9421 cagccggacc tcggaaaagg atggcgcttt catacgcatg acgttgtatt cccgtccaat
     9481 cttcggcaac gaatgggcgt ctgaattcga ggtaaatgta tagtgcgaca gctcccctat
     9541 cctgtcagcc atgtcagtgt cagcagaaag tcctagctct actccgtcaa ctagttttcg
     9601 cacatcctct ccgaacactt ctgacattct gcgggcgcag gttccgtaca ggctcttgtg
     9661 tggggtaaat gcgtgggcaa ccaagaaaag tcctccacta tcggcagcga gctgcgcaag
     9721 ctccacaggc gataaccgcg ctctctgggt ggaaagatcc atgttagaca catatcgtga
     9781 aaggctgcgc gaatactggc gcaaggcagt caaggttgga aagagactga gatagtgggc
     9841 gcctctagcc gatcccggac aggcggattc cacctctgca ccgggtatga ccgtagtctt
     9901 gtcgaggtat ctaaagcctc cgccaggcgc ctcggtcatg tcgcccgatt caagaaggtc
     9961 ctctatatct ttgagaaccc ttgttgatgc
//
LOCUS       DAOSAI010000038         9912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4476_length_9912_cov_3.482906, whole genome shotgun sequence.
ACCESSION   DAOSAI010000038 DAOSAI010000000
VERSION     DAOSAI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4476_length_9912_cov_3.482906"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1000)
                     /locus_tag="PLM83_02555"
     CDS             complement(41..1000)
                     /locus_tag="PLM83_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOK70323.1"
                     /translation="MRSSFIKRTMASIVLALVVILAFSPVPLEASLHADGVDYAASTL
                     SSGFPVTIVDDTGYRLVIKKAPARIVSLAPSNTEILFSLGLGDRLVGVTTACDYPQAA
                     AKIDKIGDYTINVEAVVAKRPDLVVAQSDLQMPVVEKLRELGITVLAVAPKTMDEVFR
                     SMEMIGAAAGEGVRARKVVSSLETRVSAVEKAVAGLPESKRPVAFVEIWNDPLMTAGA
                     GTFIDDLITRAGGRNLAGDVDGWPQISPELVVASKPDVIILTCFNKAEVMARPAWQKI
                     PAVVRGAVYELVPDLLVRPGPRLVDGLEALAAIFHPSLFGLRK"
     regulatory      complement(1186..1402)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1457..3364)
                     /gene="selB"
                     /locus_tag="PLM83_02560"
     CDS             complement(1457..3364)
                     /gene="selB"
                     /locus_tag="PLM83_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOK70324.1"
                     /translation="MRYAIIGTAGHVDHGKTTLIKALTGVDADRLRQEQERGMTIDLG
                     FAPIMLPSGRVAGVVDVPGHERFIKNMLAGATGVDVVLLVVAADEGVMPQTEEHIDIL
                     QLLGVREGVVAITKADLVDDDEWMELVVEDVKERLASTFLANAPIICVSAATRTGLDT
                     LLAAIDSAVGRAVLQEEWSFTRLPIDRVFAISGHGTVVTGTLTGGEVKPGDKLELLPA
                     GKSVRVRQVQVHDSAVDEAVRGQRVALNLAGIDRANIERGMVLAKPGMIEPARIFDAE
                     LTLLESADVIEHGERMHLHIGAAEAVCRVRTIGTDGACPGDRVFVQLELEEEIAATRG
                     DRFIVRTYSPMKTAGGGRVLAVSRGRQRRFKEETLHWFETLSGDDESATARLFASSII
                     AQKSAPASAFDVARSMCADPEHVRDIFEEGVALGWAVAFGESPGTRTYMPRDDWDKAT
                     DAIIKALSEYHRREPLRAGMAKEELRTRTMSSWDGKPFSMLIAAAARDGLIEERGTLV
                     ALPWHTVSLSPKQRDEAERLEAAYSSSLMSPPERDQALAGETSEAILDYLIDEGILVR
                     VGPELLFHRDALSRAVEVARSLGEGGKPFTAAQFRDACGNTRKYAVALLEYLDSNRIT
                     VRVGDERVLAG"
     gene            complement(3364..4854)
                     /gene="selA"
                     /locus_tag="PLM83_02565"
     CDS             complement(3364..4854)
                     /gene="selA"
                     /locus_tag="PLM83_02565"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOK70325.1"
                     /translation="MNELMRAIPSISDVLSDPAAVELMAAYPRWAVRNAARELIDSER
                     RRFSSVAGQGVCSIDVQRQDVLARVVANLPSATVLAAAPGPRYVINATGIVLHTNLGR
                     APLPEYAARAVAECAMGYSDLEYDLEGGGRGSRQDHIARAMAELTGAPAAYVVNNNAA
                     AVLLCLDTLARGRKVLVSRGELVEIGGSFRVPDIMERSGATLVEVGTTNRTRLSDYRR
                     AIDLEAGLLLKVHTSNFRVMGFTEEVQLAELVELGRECGLPVMYDLGSGLVSDLGRFG
                     LHAEEPTVIEAVASGADLVTFSGDKLFGGPQAGIILGREDLIRKISVNPLARALRIDK
                     LTLSALAAVAAAWENPDDAGESIPVLSMLTQNTDELRERAASLMGLIEGGLDEREIPR
                     NLVDLAIVDDPSEAGGGSMPTVSIPGASVSVTSRCIPADQLQTRLRRCEPPVVARIAE
                     GSVRFHMRTLFERDLKILAEHVCDILASPATFGGDTDLDFSEKEQD"
     gene            complement(4894..5916)
                     /gene="selD"
                     /locus_tag="PLM83_02570"
     CDS             complement(4894..5916)
                     /gene="selD"
                     /locus_tag="PLM83_02570"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5896..5898),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOK70326.1"
                     /translation="MSTKAGUAAKIGPGDLQMAVRGLVVRKDPLLIAGFDGSEDAGVY
                     KLTPDIAIIQTVDFFTPIVDDPYEFGQIAAANSLSDVYAMGGVPIVAMNVVCYPCSLG
                     MEVLRDILRGGADKVYESGAVLVGGHSVDDKEPKYGLSVTGTVHPDKVVLNKGARPGD
                     VLVLTKPLGTGVISTALKGEMVDPHVERYAYTVMSTLNASASAVMQEIGVHACTDITG
                     FGFLGHLAEMADASDVSFTVYADAVPVIDGALDYIDMGLAPAGLHRNWEHVGPVVEVG
                     GNVDRNIVDAMCDPQTSGGLLIALASDKADELVRRLKDAGALAAAIVGEACRRGPEGP
                     TITVVS"
     gene            complement(6104..6200)
                     /locus_tag="PLM83_02575"
     tRNA            complement(6104..6200)
                     /locus_tag="PLM83_02575"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6164..6166),aa:Sec,seq:tca)
     gene            complement(7216..8397)
                     /locus_tag="PLM83_02580"
     CDS             complement(7216..8397)
                     /locus_tag="PLM83_02580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOK70327.1"
                     /translation="MLTHRAFKVLTSFVIGVVIGVCLRTACPVEAPALSPPYLSPPGR
                     PELIYHRAFREGPAVSARAAILVEASTGIVLYAKNEHEQREQASITKAMTAIVAIERG
                     DLADIVRVSPNAAKVPGSTLNLVPGQDYKLEELLRATMLRSGNDGATAVAEHVGGSVE
                     DFVTLMNARAASMGLKNTHFANPHGLSAPGHYSTAYDIALMCMWGLRLPKFADIVGCP
                     ELWTSSVESGRAKVVYNTNRLLWGFSGADGIKTGTTSKAGHCLAASATRDGLQFIAVV
                     LRSNARFKDAANLLEYGFSTFTRIRAAEAGVAVGDVLVRRGLDRSVEVATAKDVDVVV
                     RVDEVPGISVEIRVPESPEAPVCPGDRVGWLDVLYQGDLIARQQLYAMKAVDRRPIWA
                     R"
     gene            complement(8463..8927)
                     /locus_tag="PLM83_02585"
     CDS             complement(8463..8927)
                     /locus_tag="PLM83_02585"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HOK70328.1"
                     /translation="MVAAQPERDELLNGKRLACIVLECVDSIPQPVGRTKIALLLKGS
                     RSQQIRTIDIRQVRHFGALGHLTIAQIQGIIEQLLSEGYLGSSYGKRPVIHVTSLGRT
                     TMITGDMPEVTIPDELKLYVARERCRKCPHNPYITDGPGAGPNTPNDIPPPF"
     gene            complement(8997..>9912)
                     /locus_tag="PLM83_02590"
     CDS             complement(8997..>9912)
                     /locus_tag="PLM83_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70329.1"
                     /translation="LAPVPVVWLGCKAVVGVGNRIIADLLLVPIFLCLLVAALALPYA
                     IAILVLATEPRGRRGTFYSPGNLFYDIAPGTTLREYRVAPGSVPESRKRRPSARKTFM
                     WILGVLALILTASLFLYPSYNSRRPQPVLVTHVVDGLGGHSLVFESNDNIKGISVVSR
                     SGERGPEKIDARSTSHLIPYSFSAPRLSLVAEMDGPALRVRLLSSAPIRSVKICIVGP
                     QGMAVLSSNRRCLIEEDGDMVRISTVLSGEEPIEAQFELAFPPDAPLSVEATGSFWEN
                     PAPIIISGDGKRFYETNQFVVATPITAK"
BASE COUNT         2162 a         3033 c         2585 g         2132 t
ORIGIN      
        1 gggactgtcc cgcaaatcgg ttggcgcggt tgcttgcagc ctattttctc agcccgaata
       61 ggctggggtg gaatatggcc gccagcgcct caagcccgtc gaccaaccgt gggcctggcc
      121 taacaaggag gtcgggtaca agctcgtaca cggcgccacg gaccacggcc gggattttct
      181 gccaagcggg cctggccatg acctccgcct tgttgaagca tgtcagtatg atcacatcag
      241 gcttcgatgc gaccacaagc tcgggactga tctgaggcca gccgtcaacg tctcccgcaa
      301 ggttgcgtcc gcctgcacgt gtaataaggt catcaatgaa tgtcccagct cccgcagtca
      361 tcaacggatc attccatatc tccacaaacg caacaggcct cttcgactcg ggcagaccgg
      421 caacagcctt ttccaccgca gacactcgcg tttcaaggga cgatacgacc ttccgcgccc
      481 gcacgccctc gcctgccgcg gccccgatca tctccatcga tctgaaaacc tcatccatgg
      541 ttttcggggc gaccgctagc accgtgatcc cgagctccct gagtttttcc acaacaggca
      601 tctgcaagtc agactgagcc acaactaagt ccggcctctt cgccaccacc gcctctacgt
      661 tgatggtgta atcgccgatt ttgtcgatct tggcagccgc ctgcgggtaa tcacatgctg
      721 tcgtgacccc aacaagccta tcaccaagcc cgagactgaa aaggatctcg gtgttgctcg
      781 gagcaagcga cacgatcctg gcaggcgcct tcttgatcac gagtctgtat ccggtgtcat
      841 ctactatcgt gacgggaaat ccgcttgaca gagtggaagc agcataatcc accccgtccg
      901 cgtgcagcga cgcctccaga ggcactggcg aaaacgccag gataacaacg agagcaagca
      961 cgatgcttgc cattgtccgt ttgataaatg acgatctcat tgttggacac tcctttccgg
     1021 tttggcgcat gtgcgcccgt ataggccggc gtgagccgca gcatctcgtt gcgcataaaa
     1081 aaacctgttc acccatgatg aacagggaca atcttcacgc acggcacagg ttgcagcggt
     1141 caaccgccac tgcgccatcc gcatcctccc ttttcctcga gggcggttac ggcaagctta
     1201 gtgggcaggt ttcctggctt ccggttcata gcttcggcgc gtcttcccat cgcatgagcg
     1261 aaagtgacat gcggctcgca tcagctcccc ggctacagtg gcgggaccgc gggcttcgcc
     1321 cggcgcattc tcccatgccc ggcgcctggc tgccacttcc ctttcaaccc gcatagcagg
     1381 cacccaccag ccttctatgt tcagttcgct gcgtcaattc tagcacacca catacaaaca
     1441 tccaagaccg caaggctcaa cctgccaaga cgcgttcatc ccctacccgc acagtaatcc
     1501 tgttggagtc gagatactcg aggagagcca ccgcatactt ccgcgtattt ccgcaagcat
     1561 ccctgaactg ggcggcagtg aatggcttgc caccttctcc gagactgcgc gctacttcca
     1621 ccgccctgga cagtgcatcc cggtggaaca aaagctcagg gcctactctt accagtattc
     1681 cctcatcgat caggtagtcg agtatggcct ccgaggtttc cccggcgagc gcctggtccc
     1741 gttcgggggg agacatcaat gaagaagaat acgccgcttc gagcctctct gcctcatccc
     1801 gctgctttgg gctgagtgag acagtatgcc aaggcagtgc caccaaagtg cctcgttctt
     1861 cgatgagccc gtctcttgcc gcagctgcga tcagcatcga aaatggcttg ccatcccaag
     1921 aggacatggt cctcgttcgg agctcttcct tcgccatccc tgcccgtaat ggttcgcggc
     1981 gatgatattc ggaaagtgcc ttgataatgg catctgtagc cttgtcccaa tcatcccgtg
     2041 gcatatacgt gcgggtgccc ggcgattcgc cgaaggccac agcccatccg agagccactc
     2101 cttcttcgaa gatatcgcgc acgtgctctg gatctgcgca catcgaccgg gctacgtcga
     2161 atgcagacgc aggcgcagac ttctgggcga tgatggagga agcaaagagt cgcgcggtgg
     2221 cggattcgtc atctcccgac aacgtctcaa accaatgcag cgtctcctct ttgaaccggc
     2281 gctgacgccc tctggacacc gcgagcacgc ggcctcctcc ggccgtcttc ataggagagt
     2341 aagtgcggac tatgaatcgg tcgccgcggg tcgcggcaat ctcctcctcc agttctagtt
     2401 ggacaaagac gcggtcgccc gggcatgccc catcggtccc gatcgtcctc actctgcaaa
     2461 cagcctccgc tgcaccgatg tgaaggtgca tcctctcgcc atgttcgatc acatccgctg
     2521 attcgaggag agtaagctcg gcatcgaata tccgagcagg ctcgatcatt ccaggcttgg
     2581 ccagaaccat cccgcgctct atgttggcac ggtcgatgcc agcgaggttg agagccacgc
     2641 gttgcccgcg cacggcttca tccacagcac tgtcgtggac ctgtacttgc cggacccgga
     2701 cgctcttacc cgcgggaaga agctccaact tatcgccggg cttcacctct ccgcccgtca
     2761 gcgtccctgt aaccacggtg ccgtgtcctg agattgcaaa aactcggtct atggggagcc
     2821 tggtgaaact ccactcctct tgcagcacag cgcggccaac tgcggaatct atcgcggcaa
     2881 ggagcgtgtc gaggccggta cgtgtggcgg ccgatacaca gattatgggg gcatttgcaa
     2941 gaaaagtgct tgccaggcgt tccttaacgt cttcaacaac tagctccatc cattcatcat
     3001 cgtccaccag atccgccttg gttatagcca caacgccctc gcgcacgccc agaagctgga
     3061 gtatatcgat atgctcttct gtctgaggca tcacgccttc atcggccgcc actaccagca
     3121 agaccacgtc gacgcctgtg gcccccgcca acatgttctt gatgaaccgc tcatgcccgg
     3181 gcacatcaac aacgcccgca acccgcccgg aaggcagcat gatcggagcg aagccgaggt
     3241 cgatcgtcat gcccctctcc tgctcctgtc ggaggcggtc ggcatcgacc ccagtcagcg
     3301 ccttgatgag cgtggtcttt ccatgatcaa catgcccggc agtgccgatt atcgcatatc
     3361 tcatcagtct tgctccttct cgctgaagtc aagatcagta tcgccaccga aagttgccgg
     3421 cgaagccagg atatcacaca cgtgctcggc cagaatcttc aagtctcttt cgaaaagcgt
     3481 tctcatgtga aaacgaacag agccttcggc aatgcgcgca accactggcg gttcgcatct
     3541 tctgaggcgc gtctgcagct ggtcagcggg aatacagcgg gatgtcacgg aaacgcttgc
     3601 ccctgggata gacacggtag gcatagagcc tcctcctgcc tcggacgggt catcgactat
     3661 cgcaaggtct accaaattcc gaggtatctc cctctcgtcg agtccgccct cgatcagccc
     3721 catgagcgaa gcagcccgct ctctgagttc atctgtgttt tgggtgagca tggataagac
     3781 cggtatggat tcccctgcgt catcagggtt ctcccatgcc gccgccacag cagccagggc
     3841 agacaaggta agcttgtcta tccgcagagc ccgcgcaagc gggttgacag atatcttgcg
     3901 gatcagatct tcgcggccga ggatgatgcc ggcctggggc cccccaaaga gtttgtcacc
     3961 gctgaacgtg acgagatccg ccccggatgc caccgcttct atgacagtag gctcctccgc
     4021 gtgcagaccg aatcgtccga gatcagacac aagacccgaa cctaagtcat acatgaccgg
     4081 gagcccgcac tctcttccca gttctacaag ctccgccagc tgcacctcct cagtaaatcc
     4141 cattacccgg aaattcgagg tgtgcacttt gaggagaagc cctgcctcta aatcgatggc
     4201 gcgacggtag tccgaaagac gagtgcggtt ggtcgttccc acttcgacca gggttgctcc
     4261 cgacctttcc atgatgtcgg ggactctgaa ggatccgcct atctccacta gctcgcctcg
     4321 gcttaccagc actttgcggc cacgcgccaa cgtgtcaaga cacagcagga ctgctgcagc
     4381 attgttgttt actacatagg ctgcaggcgc acctgtaagc tctgccatgg cgcgggctat
     4441 gtgatcctgt cgagagcctc gtccgccgcc ttcaaggtca tattcgagat cagaatagcc
     4501 cattgcgcat tctgccacgg cccgcgccgc atattccgga agcggagcgc gccccagatt
     4561 ggtatggagg actattcccg tggcgtttat cacataccta ggtcctggcg ctgcagccaa
     4621 gacggttgca gacggcaggt tcgccacaac acgggccaat acgtcctgcc tttgcacatc
     4681 tatggagcag accccttggc cggccacgga agaaaacctc cgcctctccg aatctataag
     4741 ctcacgggcg gcattccgca cagcccacct gggatacgcc gccatcagtt ctacggcggc
     4801 aggatcggaa agcacatccg atattgacgg aatcgcgcgc atcaattcgt tcaattcata
     4861 ttgcacctgc cgttcacaaa gtatctcggc gggtcaggag accaccgtta tcgttgggcc
     4921 ctccggaccc cgccggcacg cttctccaac tatcgcggcc gccaaggcgc ccgcgtcctt
     4981 taatcggcgc acaagctcat ctgctttgtc agacgccagg gcgatgagga gcccgcctga
     5041 tgtctgcggg tcgcacatcg catcaacgat attccggtca acattgcccc ccacctccac
     5101 tacgggccca acatgctccc aattgcggtg aaggcctgca ggggcgagtc ccatatctat
     5161 gtagtcaagt gcgccgtcaa taaccgggac ggcatcggca tagacagtga atgacacgtc
     5221 tgacgcatct gccatctccg caagatggcc tagaaagccg aagcccgtaa tgtcggtgca
     5281 cgcatggaca ccgatctcct gcatgacagc ggacgcgctg gcattgagcg tggacatcac
     5341 agtataggcg taacgctcta catgagggtc caccatctcg cctttgaggg cggtggaaat
     5401 cacgcctgta cccaagggct tcgtaaggac aaggacatct ccagggcgcg cccccttgtt
     5461 gagcaccacc ttgtcggggt ggaccgttcc cgtcactgaa aggccatact tgggctcctt
     5521 gtcatccacg gagtggcccc caaccagcac cgcgccggac tcgtatactt tgtccgctcc
     5581 gccccggagg atgtctcgaa gaacctccat cccaagcgag caaggataac atacaacgtt
     5641 catagcgact atggggacgc ctcccatggc atacacgtca gagagagaat tggcagccgc
     5701 tatttgacca aattcatacg gatcatccac tatcggggtg aagaagtcga cagtctggat
     5761 gatggctatg tcgggagtca gcttgtagac gccggcatct tccgatccgt cgaaccccgc
     5821 gataagcagc ggatccttgc ggacaacgag ccctctaaca gccatctgta ggtcacccgg
     5881 acctatcttg gcagctcagc cggcttttgt tgacattgtg gtaagtctga tgcgatcagt
     5941 ctctgtcacc ttctgtcact cctcgcaaaa caaacttgaa cccataccct actctagttt
     6001 acccgcgcca gtctgtccat ctcttgggca aataggccgg tgtgccacgg acgcgcatcg
     6061 aggaccgcgg ggatcagcta gtggggcaca ttgggcatga aaatggcggg agtacgtggg
     6121 aatcgaaccc accgcggcca ggttagctgc ccgccactgg atttgaagtc caggaggccc
     6181 accaggacca atctactccc gcgtgaaaga ctgagatgtg tatgttccca ttataaccgt
     6241 acatcctttc ctcgtcgagg gggcaccgaa tctaagccct tacgcgctgt cccgttttgc
     6301 gattggcaaa ggccgaaaat gccaggctgg tgccgaggta tctgtgcgga gcaaactgcc
     6361 tgggtcgccc ggggtgattc cggcaatcct ccttcacttc catgccaaaa tccttgtccc
     6421 caacgcattc ttatacgcat tagcatgact tgagtaggtc tcgacaagtc tctgcacgat
     6481 tccgaagtcc gctttacatg cggtttgaac ctgaacccca ctttatcagg acacgatgct
     6541 gctcaggcat gtttccggag tgtatgggga gaagtagagg ttggcaacga acctggtcaa
     6601 ctttgagtca catcccattg aggcgaacca gcggggattc tcgtggatat gttcaatgcg
     6661 taacgctcgc aaaagccagt ccaacgcgtg ccaacaggct ttttttgagt tttccccaaa
     6721 agcccatacg tgtgcaggca aaacccaaga tcaagctgtg ccgattactt gacctgcctt
     6781 cgctaaaaac aacctcgaac tgagctttat acacattccc atcgggcaac tttccctgtg
     6841 cggtatccac gcgataggcg tttgaaaggg ttcagctact ctttcaaagc tacttgttgt
     6901 cgcagaccgt tgcgggacca attcgcgctc ttatgcacat tcagctgact gacatcaccg
     6961 tcaaattggg cagcacaaag ctgtaaaccg ccaacaagct atctgcgcca cacggaacgc
     7021 gtagtcttgg atagcagaat aacgctttac agtatgttcc tttacttcaa ccccaagcca
     7081 tccagcgcga ttcttgaccg gccgcacagt cagtaacagt cagccgaagc atgcaggaga
     7141 accaattgcc cttgccatca gtcctcatgc ggatctttga ctggaagccg cttgcggccc
     7201 gcagtttggc agacatcaac gtgcccatat aggcctgcgg tcgacagcct tcatagcata
     7261 cagttgctgg cgggcaatga ggtcaccttg gtatagcacg tcgagccacc ctacgcgatc
     7321 ccctgggcat actggcgctt ccggcgattc gggcaccctt atttccacgg atattcccgg
     7381 cacctcgtca acgcggacca ctacatcgac atccttcgca gttgcaacct ccacagaacg
     7441 atccagtcca cggcggacga gaacatcgcc cacagccact ccggcttcag ccgcccttat
     7501 ccgcgtgaac gtggagaaac cgtattccaa caggtttgcc gcatctttga atctggcatt
     7561 ggagcgaaga acgactgcta tgaactgcaa cccatcccgg gtcgcagagg ctgccaagca
     7621 atgtcctgct ttggaagtag ttcccgtctt gatcccatcg gcgccagaaa aaccccaaag
     7681 aagcctgttg gtgttgtata ctactttcgc cctcccgctt tccacagaag atgtccaaag
     7741 ttcagggcat cccacaatgt cggcgaattt gggaagcctc aacccccaca tgcacatgag
     7801 ggctatgtcg tacgctgtgg aatagtgtcc aggagcggat agtccgtgcg ggttggcgaa
     7861 atgggtgttc ttcaagccca tgcttgctgc gcgggcattc atcaacgtca cgaaatcttc
     7921 taccgatcca cctacatgct ccgccacagc cgttgcgccg tcatttcctg acctcagcat
     7981 ggtagcgcgg agtaattcct caagcttgta atcttgccca gggacaaggt tgagtgtcga
     8041 tccgggaacc ttggctgcat tcggtgatac gcgcacgata tcagccagat cgccccgttc
     8101 aatggcgact atggctgtca tggccttggt tatgctcgcc tgttctcttt gttcatgttc
     8161 gtttttggca tagagcacga tcccggtgga ggcttcgact agtatggcgg ctcgggcgct
     8221 caccgcaggt ccttcgcgga aagcgcggtg gtagatgagt tcgggacgcc caggtgggct
     8281 gaggtatggg ggcgacagtg cgggcgcctc aacgggacag gctgtgcgca gacagactcc
     8341 gatgaccacc ccgataacaa atgatgttaa aaccttgaag gctctatgag tgagcaattt
     8401 ggcccagcgc ccccgatcag cccaagttga ctgccttaat tcttacgcca agcgaagtct
     8461 agctagaacg gaggaggtat gtcattgggt gtgttagggc ctgcacccgg gccgtcagtg
     8521 atatagggat tatgagggca tttgcgacat cgttcccgtg ctacatagag tttgagctcg
     8581 tcagggatgg tgacctcggg catatcgcct gtgatcattg ttgtacgtcc caacgacgta
     8641 acatggatca ccggtcgttt cccataggat gatccgaggt atccctctga aagaagctgc
     8701 tcgattatcc cttggatctg ggcgattgta agatggccca aggcaccaaa atgcctgacc
     8761 tgtctaatgt cgattgtgcg aatctgttgt gatctagacc cctttaacaa gagagctatc
     8821 ttggtgcgtc cgacaggctg aggtattgag tccacacatt ccagcacgat gcaggcaaga
     8881 cgcttgccat tgagcaactc gtcacgctcc ggctgtgccg caaccacatc gtcacctcca
     8941 gtataagtta ttgcaccaac agattaccat aggtcccttt agatgtcaac tgtatcctat
     9001 tttgcggtta taggcgtagc aactacaaac tgatttgttt cataaaacct cttaccatct
     9061 ccagatatta taattggagc agggttctcc cagaatgatc ctgtcgcttc cactgagagc
     9121 ggtgcgtcgg gcggaaacgc caattcgaac tgggcttcga tcggctcttc tcccgataac
     9181 acggtgctga ttcgcaccat atcgccatct tcttcaataa gacagcggcg gttgctggag
     9241 agtaccgcca tcccttgagg acctacaatg catatcttaa cggaacggat aggggcactc
     9301 gataacagcc taacccggag cgcgggaccg tccatctctg caacgaggct cagccgcgga
     9361 gcactgaatg aatatggtat cagatgcgag gtggatctcg cgtctatctt ttcggggcct
     9421 ctctctccag agcgggaaac gacagatatc cccttgatgt tgtcattgct ttcaaatact
     9481 aggctatgcc ctccaagtcc gtccactaca tgcgtaacca atactggctg gggccttctc
     9541 gaattatagc tggggtacaa aaacaggctg gcggtcaata tcaaagccaa cacacccaga
     9601 atccacatga aagtcttgcg agcggagggc ctcctcttcc tgctttctgg gactgaacct
     9661 ggagcaactc tgtattctcg cagtgtcgtg ccgggggcaa tgtcgtagaa caggttgcca
     9721 ggggaataga aggtccctct ccttcccctc ggttccgttg ccaggacgag tatggcgatt
     9781 gcatatggca gcgcgagcgc cgcaacgaga aggcagagga atatgggaac caacaagagg
     9841 tccgcaataa tccggtttcc cacgccaaca acggccttgc acccaagcca cactacgggc
     9901 accggagcaa gg
//
LOCUS       DAOSAI010000039         9758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4566_length_9758_cov_3.792848, whole genome shotgun sequence.
ACCESSION   DAOSAI010000039 DAOSAI010000000
VERSION     DAOSAI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4566_length_9758_cov_3.792848"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLM83_02595"
     CDS             complement(<1..292)
                     /locus_tag="PLM83_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70330.1"
                     /translation="MALPQPNKESDSIVVVKGLTKHFSVGRGLFRREQARLHAVDDVS
                     FDVARGEVFGLVGESGCGKTTTGRMLVKLTVPTSGDIFVDNEDISAISGQGMR"
     gene            complement(273..1277)
                     /locus_tag="PLM83_02600"
     CDS             complement(273..1277)
                     /locus_tag="PLM83_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70331.1"
                     /translation="MDALLKIENFTMHYRTRAGHVKAVDDVTFSLAHGQSLGLVGESG
                     CGKTSVAMSIMRLLPYNSEIKSGTIVLHGQDIYSMREEEFRKLRWKEVAMIFQAAMNA
                     LNPVYTVGSQITEVILEHEPEVSEREARQRVTELFSTVGIPEERMDHYPHEYSGGMKQ
                     RAVIAMALACNPSLIIADEPTTALDVIVQDKILREMEEIRRKTGMSMMYISHDVSVIA
                     EVSDIVGVMYAGKLVEIGPADDVFKRPRHPYTYGLMNAYPSILGEKRDLATIPGEPPN
                     LMFPPLGCRFHPRCDWATDECGQVEPRARYVGPDHSVACHNPLRAPSSGGEPIGVATA
                     "
     gene            complement(1285..2280)
                     /locus_tag="PLM83_02605"
     CDS             complement(1285..2280)
                     /locus_tag="PLM83_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012004036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70332.1"
                     /translation="MMTDPAKNPNIHPSSGMGPTAHSRVIREPLWKIRWRLNMRSLAQ
                     NWAVFKESRIGLAGLAIIVCFGVFGLMWPIMIGNLWDVHIYHPVVGFDPAMMHPAPPC
                     RGHVLGTDPIGRDVLAQLMFSTGREFLLGVLSAVISVVVGTFVGAIAAYYGGLTDSFF
                     MRLADIIMLFPFVPLLVVVGSLVRLDMFSFAIILGFLEAFGGITIILKAQALQVNVRP
                     YIDAAKVAGGDDLHIIRSHIIPNVLPLSFLYMMFNVTAAILSEATLSFFGVLNIDMSW
                     GMMIHTAETAGYLFDYGRYWWLWLPSGLAITLFCAAFYMVGRGLDEVVNPRLRKR"
     gene            complement(2288..3466)
                     /locus_tag="PLM83_02610"
     CDS             complement(2288..3466)
                     /locus_tag="PLM83_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70333.1"
                     /translation="MRRYVLSRVGQLVITLLIYITLVYFLLQAMPGDISMIYLQNPRI
                     PQAAREELKKQLGLDRSPVMQYFSYMKNFFTGNLGVSFSQYPRPVWDILIERLPRTLV
                     LFLTANSLSFYVGFVLGRVIAWKRGASIDHVATVVGITFWTAFYPLLALVLIWLFAYT
                     LKWLPLNQFIEPSLWVHAPWPSNTVFGFMVLGVVLISVSIFIIFWRLTRGQRKTRRNI
                     GLAWGLSAAVVAIALGVAAKAGMLIYIWDIVRHMILPVVTLTLINFAGTMLLMRDSML
                     ETVEEDYVMAARAKGLPDAVVRDRYAARNALLPTVTSLVLSLASSMSGGLVTETMFSW
                     PGLGQVLFRSALANDYPLATGALVFTGIFLLIAHFVADMLYAALDPRISYTGEPAAEG
                     "
     gene            complement(3581..5455)
                     /locus_tag="PLM83_02615"
     CDS             complement(3581..5455)
                     /locus_tag="PLM83_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK70334.1"
                     /translation="MKVVRCIITVLLFMVVVFGMGLIGQAAAGGVYEMAIFEDPTTVN
                     VWAALGPDATVWNAFVGYQQSYIGLYGNAAPDFVFCPVAASGMPSPFKEEKIGDKTYY
                     TSVVNIRKELKWSDGRPLTADDVVFSYMAPLALDPNRLGGNWPGNIDPEILAKVEKVG
                     EYSVKFYLTELPGLAQWQYGMLQALILSKAYWDPVVSRALKAADPVKELLAHDPKGEP
                     VAGPWVRDKWEKGAFVTHVPNPYYAMKGDTNTFYKNGAFVTENKKTGFSYVLGDPKGD
                     VKLSVTEGPYANRLMFRILQNQNAAVLSLMAGEVDFVLNSLGLQRGFQDQLKKAANVE
                     VVENNVNGFRYMAFNLSKYPFGIKEFRQAVSVLIDREYICERVLQGVAFPQYSVVPPG
                     NAYWFNSNVPMWGKGMTRSQRITEAVRLLKSAGFSWEVEPQVDLAKDKVLRRGKGLIM
                     PNGKKMQPFEFNVMTAGYDPLRYTFGLNIATWMQEIGIPVEVVPTEFNVVISKTDERD
                     FDCFMLGWSLSLYPDHMYWFFHSSQCPAGGFNYEMYNNPKFDKMVEDFLKESDMETAR
                     KMAFKLQEILADELPYIVLFDTPLIEAYRSDRLAYPYTKTLSGIQYNGGMTSTVRLKN
                     "
     gene            complement(5567..6094)
                     /locus_tag="PLM83_02620"
     CDS             complement(5567..6094)
                     /locus_tag="PLM83_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOK70335.1"
                     /translation="MCNMRRRTMGPAIALMSMLMLLAGHGSTVLAQPSTLVGSRAPEI
                     KAVGVRNESVQVPARGGITVLVFWNSKYRLSLDALAAIQGIWEAYGARGVVCVGLNDL
                     GEEDRAVEQLAERMNIGFVLVSGRTASSAAVAYRLRGVPAVFVVDRSGVIVYVREGWD
                     KKAESEIREIISKAL"
     gene            complement(6341..7393)
                     /locus_tag="PLM83_02625"
     CDS             complement(6341..7393)
                     /locus_tag="PLM83_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOK70336.1"
                     /translation="MVDRIAKTQYASELRPGTVVNSVFLCADVKVRPTKSGSQFADVR
                     LVDRTGEISLKLWNYSENVAGALAPGAFVKIRNVPVTEYNGSLQLSCETAQFGGAITL
                     CSSSECNLADYVPSTVRDVDSMIGEIDNAICNIKPGPIKCLLQSFFGMPEFRGEFMEW
                     PAAVRHHHAYSGGLLEHTTGVLRLCLCAADQYEFVDRDILIAGALLHDVGKLRSYDYD
                     RTHGAATMSLPGVMTDHIVIGVEIVNRRIADLLNGCENKGSCAGPCWTEQATLHITHL
                     IASHHGSLEWGSPVQPATIEACILHHADNMDAQVNKYERTIREMLGTGDGQCRYSEHV
                     GKMVWAPPGSFEDDRS"
     gene            complement(7403..7825)
                     /gene="ndk"
                     /locus_tag="PLM83_02630"
     CDS             complement(7403..7825)
                     /gene="ndk"
                     /locus_tag="PLM83_02630"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOK70337.1"
                     /translation="MERTCVLIKPDGIARGLVGEIVSRFERRGCRIVMATTVNMTEEL
                     LAEHYAHLIGKPFFADLKDYMMSGPVIALVLEAPNAVEAVRQIIGATDPIKAGAGTVR
                     GDFATSLHRNLVHASDSLDAAAAEIARFFPRVALQNTK"
     gene            complement(8012..9202)
                     /locus_tag="PLM83_02635"
     CDS             complement(8012..9202)
                     /locus_tag="PLM83_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70338.1"
                     /translation="MKQGLFASGLFLLAAAIVVAYTPAAWAEEALALDAETSTSEGGS
                     SCRTIGVGEFFTAAMSCDPGFLSAWIDTNETRASQAAGQSWTHSTTLWGSDSAIRGSI
                     LATGRSGGRVEFSYDCESSIDSHVQGARIGVEVPLSALADGVLCRRSDSIPTHLHVVE
                     ARLARAEGEAVAGALGCLRRAQIASFMGDMNEAQRAHVELSLRLGCRDGEEIIQALCQ
                     EEFDHVAAMIGSIINSDAGALCEEWIRRDPKVLEAKIDLAAKHESGLAEMLQATSFVA
                     EAKWHALEPNRRCAIGFSLNGRGLARTVGASAEMLEARVEAEQARSTIDFLQVFEAVA
                     EAEAACGFLHGVEGMEADYLEAVLRLEQNGVRLMAGLGMLPYSFLANPHVSPAQRILA
                     GCVK"
     gene            complement(9254..9505)
                     /locus_tag="PLM83_02640"
     CDS             complement(9254..9505)
                     /locus_tag="PLM83_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70339.1"
                     /translation="MDRWLDNIVMAELCPAIIDVKIGVFCFCLAVIFGYPCPSDAELR
                     HHPGCNARFTAAKRHAAASAKPACGCGTGKITLKGDGKT"
BASE COUNT         2423 a         2919 c         2260 g         2156 t
ORIGIN      
        1 cgcgcattcc ctggccactg atggccgaaa tgtcctcgtt atccacgaat atatcgccgg
       61 atgtagggac agtaagcttg accagcatcc ttccggtagt cgtcttgcca cacccggatt
      121 cgcccaccaa gccgaagact tccccgcgag cgacatcgaa actcacgtca tccactgcat
      181 ggagacgagc ctgctctctt ctgaacaatc ctcggcccac agagaagtgt tttgtgagtc
      241 ctttgaccac tacaattgaa tcagattcct tgttaggctg tggcaacgcc aatgggctca
      301 cctcccgacg atggagctcg aagtggatta tggcatgcaa ccgaatggtc cggtcccacg
      361 taccgcgctc gcggttctac ctggccgcat tcatcagtcg cccaatcaca cctgggatga
      421 aacctgcatc ctaggggcgg gaacatcaag ttggggggct cgcctggaat cgttgccaga
      481 tcgcgttttt ccccaagtat gctgggatac gcattcatca gcccgtaagt gtacgggtgc
      541 ctcggacgct tgaacacatc gtctgcaggt ccaatctcca caagttttcc ggcatacatc
      601 acgcctacga tgtcgcttac ttcagctatt accgacacat cgtgagagat gtacatcatg
      661 ctcatgccgg tcttgcgcct gatctcctcc atctcgcgca aaatcttgtc ctgaacaatg
      721 acatcaagcg cagtagtggg ttcatcagct ataatcaagc ttgggttgca ggctaaagcc
      781 atggcaatca cagcgcgctg cttcatgccg ccgctatact cgtgcgggta atgatccatt
      841 ctctcttccg gaattccaac agtgctgaac aactcggtta cccgctgccg agcctctctt
      901 tccgagactt ccggttcatg ttcgagtatg acctctgtta tctgacttcc aaccgtatag
      961 acaggattga gggcattcat ggctgcctgg aatatcattg caacctcttt ccatctgagc
     1021 ttgcggaact cttcttcgcg catcgaatat atgtcttgcc cgtgaaggac aatcgtaccg
     1081 cttttgatct ccgaattgta agggagcaac cgcattatcg acatggcaac cgaggttttc
     1141 ccacatcccg actcgccaac aaggcccagc gactgcccgt gcgcaagcga gaacgtcaca
     1201 tcatccactg ccttcacatg tcccgccctt gtccgataat gcattgtgaa gttctcgatc
     1261 ttcaaaagag catccattct cagctcacct ctttctgagt cggggattaa cgacctcatc
     1321 aaggccccgg cccaccatgt agaaagcggc gcagaacagc gtgatcgcca gcccagaagg
     1381 gagccacagc caccagtatc ggccgtagtc aaacaggtac ccggccgttt ctgccgtgtg
     1441 tatcatcatc ccccagctca tgtctatatt cagcacgccg aagaaagaca gagtagcctc
     1501 gctcagaatc gcagctgtaa cgttgaacat catatatagg aaggaaagcg gcaggacatt
     1561 gggtatgata tgtgatcgaa ttatgtgcaa gtcgtctccg ccggcaacct ttgccgcgtc
     1621 tatataaggc ctcacattaa cttgcaatgc ctgagccttg aggatgatag taatgccgcc
     1681 aaacgcctcc aagaatccca aaataatggc gaatgagaac atatcaagcc tgacgaggga
     1741 tcccaccact accaagagag ggacaaacgg aaaaagcatg atgatatccg caagcctcat
     1801 gaaaaaggaa tccgttagcc ccccgtagta ggcagcaata gctccgacaa aagtccctac
     1861 aactacacta attactgcgg acagtactcc cagcaagaat tcccggcctg tgctgaacat
     1921 gagctgcgcc aatacgtccc ggccaatggg gtcggttcca agaacatgtc cgcggcaagg
     1981 cggggcggga tgcatcatcg caggatcgaa acccactacc ggatggtata tgtgcacatc
     2041 ccataagttc ccgatcatta tcggccacat cagcccgaaa actccgaagc aaactatgat
     2101 ggccaggccc gcaaggccaa tccggctttc cttgaaaacc gcccaattct gcgcaagcga
     2161 gcgcatattc agcctccacc tgatcttcca tagcggctcc cttatcaccc gagaatgcgc
     2221 cgttggcccc atacccgagc taggatggat gttcggattc tttgcaggat cagtcatcat
     2281 tactcaccta cccttcagcc gccggttccc cagtatagga aattctcgga tccagagcag
     2341 cataaagcat gtctgcaacg aagtgggcta tcaaaaggaa tatgcctgta aacaccaatg
     2401 cccctgtggc aagcgggtaa tcattggcca aagccgaacg gaaaagaacc tgacccagcc
     2461 cgggccatga gaacattgtc tcagtcacca agcccccact catcgaggat gcaagcgaaa
     2521 gcaccaagct cgtaacggtc ggaaggagag cattcctcgc ggcataccta tcacgcacca
     2581 cggcatcggg gagccccttg gcccgggcgg ccatcacgta atcctcttcc acagtctcaa
     2641 gcattgagtc acgcattagc aacatagtgc cagcaaagtt gatcagagta agagtgacaa
     2701 cgggaagtat catgtgccgc acaatatccc agatatatat aagcatgccc gccttagcgg
     2761 caaccccgag cgcaatggcg actactgcgg cggacagtcc ccaagccaag ccgatgttcc
     2821 gtcttgtctt gcgctgccct cgagtaagac gccagaatat gatgaatatg gacacagaga
     2881 tcaagactac tccgagaacc ataaacccaa agactgtgtt tgaaggccac ggagcatgca
     2941 cccataaact aggttcaatg aactggttaa gcggcaacca tttcagtgta taggcaaaca
     3001 accagatcag gaccaaagca agcagcggat agaaagcggt ccaaaacgtt attcccacaa
     3061 cggtggccac atggtcgata ctggctccac gcttccaggc aatgaccctg cccaacacga
     3121 accctacata gaacgacaat gagttcgcag taaggaataa caccagtgtc ctaggcaacc
     3181 gttcgatgag tatatcccag acaggcctcg gatactgtga aaacgaaacc cccaaattgc
     3241 ctgtgaagaa attcttcatg tacgagaagt actgcatgac cggcgaccga tccaaaccca
     3301 gctgtttctt gagttcctcc cgcgccgcct ggggtattct cgggttctga agatatatca
     3361 tggagatgtc tcccggcatc gcctggagca agaagtagac tagtgtaatg tagatgagca
     3421 aagtgattac cagctggccc acacggctaa gaacatatct gcgcatcttg atcacctatg
     3481 ctttccggtg ttctttggga aacgaggcgc gggccgagag gcctgaggaa ggcctcggcc
     3541 cgccccgcgc atagccagga accatatgcc attccctgga ctagttcttc agcctgaccg
     3601 tgctggtcat gcctccgttg tactgaatgc cacttagggt cttggtgtag gggtatgcaa
     3661 ggcgatcact tcgatatgct tcgatcagag gagtgtcgaa taggacaata tacggaagct
     3721 cgtcggccag gatttcctgg agcttgaacg ccatcttgcg agcggtctcc atgtcagact
     3781 ccttcaggaa gtcctctacc atcttgtcaa acttcggatt attgtacatt tcatagttga
     3841 agcctccggc cggacactgg ctgctgtgga agaaccaata catatgatcc ggatagagcg
     3901 ataggctcca accgagcatg aagcagtcaa agtcacgttc atcagttttg gatataacaa
     3961 cattgaactc agtgggaact acctccaccg gaatgccaat ctcctgcatc caggtggcaa
     4021 tgttcaaccc gaaggtgtag cgaagcgggt catatcctgc agtcatcacg ttgaactcaa
     4081 atggctgcat cttcttgcca ttcggcataa tcaggccctt gccgcgtctg agcaccttat
     4141 ctttggcaag atcaacctga ggttccactt cccacgaaaa cccagcgctc ttgagcaacc
     4201 taacagcctc agttattctc tggctacgag tcatgccctt cccccacatc ggaacattgg
     4261 agttaaacca gtacgcattg ccaggaggca caactgaata ctgggggaat gcaacgccct
     4321 gcagcactct ctcgcaaatg tactccctgt cgatcaggac tgacacggcc tgccgaaatt
     4381 ccttgatgcc gaaaggatac ttgctcaaat tgaacgccat gtatctaaac ccgttaacat
     4441 tattctccac gacctcgacg ttggccgcct tcttcagttg atcctggaat ccccgctgaa
     4501 gtccgaggga gttaaggaca aagtcgactt cgccggccat gagggatagc accgcagcat
     4561 tctggttctg caatatccgg aacatcaacc tgttggcgta tgggccctcg gtcacagaaa
     4621 gcttcacgtc gcccttggga tcccccagca catagctgaa tcctgtcttc ttgttctccg
     4681 tcacgaacgc gccatttttg tagaaagtat tggtgtcgcc cttcatggcg tagtaaggat
     4741 tgggaacatg ggtaacgaaa gctcccttct cccatttgtc cctcacccac ggcccggcaa
     4801 caggctcacc cttaggatca tgagccaaga gttccttcac agggtcagcc gctttgagcg
     4861 cgcggctcac tacaggatcc cagtaggcct tggacaaaat aagggcctgc aacattccgt
     4921 attgccactg agccaagcca ggaagctctg tcagatagaa cttgacactg tactcgccga
     4981 ccttctccac cttcgcaagg atttcggggt caatattccc aggccagttt ccgcccagcc
     5041 tgttcggatc cagagcgaga ggcgccatgt agctgaagac gacgtcatca gcggttagtg
     5101 gtctcccatc gctccacttc agctccttgc ggatgttcac aactgaggtg tagtaggtct
     5161 tgtcgccaat cttttcctct ttaaaaggag atggcattcc cgaagcggct acagggcaga
     5221 acacgaagtc aggagctgca tttccgtata gacctatgta gctctgctgg tacccgacaa
     5281 atgcgttcca taccgtagcg tcggggccca aagcggccca aacgttcacc gtagtcgggt
     5341 cttcgaagat ggccatctcg tagactccgc cggcagccgc ctgcccgatt aagcccatgc
     5401 cgaatacgac caccataaag agcaggaccg taattatgca ccgcacgact ttcattgccc
     5461 aactgcctcc ttgtcgcggt aatagtctcc tgctcttgtc tattcggcct tcgtatggaa
     5521 atcccttccg aatattatcg taaataatcc atattatcca ttataattag agggcttttg
     5581 atattatttc cctgatttcc gactccgcct tcttgtccca tccctccctt acatatacga
     5641 tgacgcccga tctgtccacc acaaacactg caggaacgcc gcggagcctg taagcgactg
     5701 ccgccgacga tgccgtacgc ccactcacaa gcacgaagcc tatgttcatg cgttctgcaa
     5761 gttgctccac agcacgatct tcctcaccca agtcattaag gcccacacac acgacacccc
     5821 gggcgccgta cgcctcccat atcccctgga tggccgcgag ggcatcaagc gacaatcggt
     5881 atttgctgtt ccagaagacg agaactgtaa ttccgccgcg cgctggcacc tgaacgcttt
     5941 catttctgac cccaaccgcc ttgatttccg gggcccgaga ccctacaagg gtggaaggct
     6001 gcgccagaac agttgaccca tggccagcaa gtaacatcaa catgctcata agagcaattg
     6061 caggccccat agtgcgcctg cgcatgttgc acattgcaaa tcacctcaaa gcaacgtctt
     6121 ccttcaatct aaggacattt gtttacacta agtaagacgc cccaacccgc atggagtttc
     6181 gaagcaaacg cgttttttta cagacgtttt cccccataat agcaacccgc tactagatta
     6241 gtcgacaagc aagcgcgaag ccatcagcat ccagagattg gaacgcccgt acgcacaaac
     6301 agggccccca tcgaaatgag agccctggac gcatcagatc tcagcttcta tcatcttcaa
     6361 aagaaccggg aggcgcccat accatcttgc caacatgctc agaatatctg cactgcccgt
     6421 cgcccgttcc aagcatctct cttatagtcc tttcatactt gtttacctgg gcgtccatat
     6481 tgtcggcatg atgaagaata catgcttcta tggtagccgg ttgcactgga gatccccact
     6541 ccagacttcc atgatgactc gcaatgaggt gcgtaatgtg cagagttgcc tgctcagtcc
     6601 agcatggccc ggcacatgac cccttattct cacacccatt gagaagatca gctattcgcc
     6661 tgttcacaat ctcaacgcct atgacaatgt ggtcagtcat cacgccaggc agagacatgg
     6721 tggcggctcc atgggtcctg tcatagtcgt aacttcgcaa cttgcccacg tcatggagaa
     6781 gggcacctgc aattagaata tcgcgatcga caaactcata ctgatccgct gcgcacaagc
     6841 acaaccttaa aaccccggtc gtgtgttcga gcagccctcc cgaatatgca tgatggtgcc
     6901 tgactgctgc aggccattcc atgaactccc ccctgaactc aggcatgcca aagaaggact
     6961 gaagcaggca tttgatggga ccaggtttga tgttgcatat agcattgtcg atctccccaa
     7021 tcatcgaatc gacatcacgc accgtagaag gaacatagtc ggccagattg cactctgatg
     7081 aactgcagag agtgatcgcc cctccaaact gagctgtttc acatgataat tgcagggagc
     7141 cattgtattc ggttacaggc acatttctga tcttcacaaa ggcgcctgga gccagcgctc
     7201 ccgcaacatt ctcagagtaa ttccaaagct tcagactaat ctcgccagtc cggtccacaa
     7261 ggcgcacgtc ggcaaactgt gagcccgact tcgttggcct caccttcaca tcggcgcaga
     7321 gaaagaccga gttcacaacc gtacccggcc ggagctccga tgcatactgc gtttttgcaa
     7381 tgcgatcaac catctgaaaa cctcatttcg tattctgcag cgcaacacgg gggaagaaac
     7441 gcgcgatctc agcggccgca gcgtcgagac tatccgaagc atggacaagg ttacggtgta
     7501 atgatgtcgc aaaatcacca cgcacagtac ctgcacccgc cttaatcggg tcagttgctc
     7561 ctattatctg tctgaccgct tccaccgcat tcggcgcttc cagcactaac gcaatcacag
     7621 ggcccgacat catatagtct ttcaggtcgg caaaaaacgg ttttcctatc agatgggcat
     7681 agtgctccgc caacaactct tccgtcatgt tcacggttgt cgccattaca atccgacaac
     7741 ctctgcgctc aaacctagac actatttcgc ccaccaaacc ccttgctatt ccatctggtt
     7801 ttattagaac gcacgtacgc tccacagcac acctccatga acacaccgga tctttcgtct
     7861 attgggtaaa gtgtgcccaa ttccgcatcg gcacacgcca cctgaggtgc aaagcccgcg
     7921 tggccacgac tcgccccctg ttgccgcgca aatttgccca accgattcca tgctcggtcg
     7981 gcttacacgc atctgccagc atatgtagac gttatttgac gcaaccggcc aaaatcctct
     8041 gtgccggcga cacgtgagga tttgcaagaa aagagtacgg cagcattccc aatccggcca
     8101 tgagacgcac tccgttctgt tcaagtctga gcacagcctc gagataatcg gcctccatcc
     8161 cctccactcc atggaggaat ccacaggcag cctcggcctc ggcaacagcc tcgaacacct
     8221 gaaggaagtc tatggtgctc cgggcctgtt cagcctcgac cctggcttcg agcatctccg
     8281 cagaagctcc gacggtccgt gcaaggccac ggccgtttag ggaaaaccca atggcacatc
     8341 gtctgttcgg ctccaatgca tgccacttgg cctctgctac aaaagatgta gcctgcagca
     8401 tctctgctaa gcccgactcg tgtttggcag caagatcaat cttcgcttcg agcacctttg
     8461 gatcgcgccg gatccactcc tcgcaaagcg ccccggcatc agagttgatg atagagccta
     8521 tcatcgccgc cacatggtcg aactcttctt ggcacaacgc ctgaataatc tcctccccat
     8581 cgcggcaacc aagcctcaaa gaaagctcta catgagctct ctgagcttca ttcatgtccc
     8641 ccatgaaaga tgcaatttga gcccgcctca aacaacccag agcgccagcc acggcctcac
     8701 cctccgccct agccagcctc gcctctacaa catgcagatg tgtagggata gagtcagagc
     8761 gtctacaaag caccccatca gccagtgcag acaggggaac ttcaacgccg attctcgctc
     8821 cctggacatg actgtcaatg ctgctttcgc aatcatagga gaactctacc cttccgcctg
     8881 accttcctgt cgccaaaatc gagcccctta ttgctgaatc cgatccccac agcgtcgttg
     8941 aatgtgtcca cgattggcca gccgcttgac tagcccttgt ctcattcgta tctatccacg
     9001 ccgaaagaaa acccggatca cacgacatgg ccgcggtgaa aaactcccca acccctatgg
     9061 ttcgacatga agaaccgccc tcgctggtac tggtctccgc atccaaggct aacgcctctt
     9121 cagcccaggc cgcaggcgtg tacgcaacca cgatggcagc agccaacagg aagaggcccg
     9181 aagcgaatag gccttgtttc acttccgaat tcccccttaa tgccattagt atatcaaagg
     9241 ataggtgaat tcttcacgtt ttgccatcgc cttttagggt gattttcccg gtcccacatc
     9301 cgcaggccgg ctttgctgac gcggcggcat ggcgcttcgc agcggtaaag cgggcgttgc
     9361 atcctggatg atgccgcaat tcggcgtcag aaggacatgg ataaccgaag atgactgcga
     9421 ggcaaaagca aaagactccg atcttcacat caataatggc ggggcatagc tccgccatta
     9481 caatattgtc caaccaccta tccacgggca gaacctgcgc cacctaagta ggtctcgata
     9541 agtttctgtg cgtttctaga gcttgcattg cctgcagtca cagccaagag gctttatggt
     9601 ttgatggatg cgctgatcga tgctgtgagc gcttttctcg acaacctgac tacgtctgaa
     9661 attcagacct tggccattta agaatgctca gatgcttttt cgaagttgct caggatccag
     9721 aaaaccgaag ccaaaccgta gccaaccgaa cctgaagt
//
LOCUS       DAOSAI010000040         9708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4591_length_9708_cov_3.328188, whole genome shotgun sequence.
ACCESSION   DAOSAI010000040 DAOSAI010000000
VERSION     DAOSAI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4591_length_9708_cov_3.328188"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PLM83_02645"
     CDS             complement(<1..872)
                     /locus_tag="PLM83_02645"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOK70340.1"
                     /translation="MNPGPMRKAYYFSTRDLVLIAAISAAGGVLSTYVGYLGNLMNKA
                     FGVPFGAGQWMAGLHVFWFVFARAIVGRTGAASLTGILKGIVEMFAGSTHGFPIVIVS
                     AVEGILVDVVLLPSGSNPSLGAMCLAGGISSASNVLIFQALYFSGVSMGYVGLITAFA
                     FASGAAFGGYFTAGVLDAVSSAHILKVDEERRSVHRPPVVRMGVAIALVLVLAGGAVY
                     YYACVFEPFWNGPTCRVEGTVAQAYNYRPIDFEDDNVTVVAELKGAVTHVPPTDYTGV
                     PLTAILERAGVGESA"
     gene            complement(946..1941)
                     /locus_tag="PLM83_02650"
     CDS             complement(946..1941)
                     /locus_tag="PLM83_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70341.1"
                     /translation="MRKNRLALVVFLAFVVGFMAMPVFWMPAQAQNNWKVEVVSGQRS
                     VTLTETDFGRMKPVSAKAAFLRTTGRIEGPALYKGVPMEEILKKVGGIDQGQAIRVTS
                     RDGYAMTYSYAQVCGGILTYDNQQRALRIGGVAMYLAYESDRDSADKLPRIVFMDKEK
                     TAITDGHFWAKSVAKIEVVPGVDDWMIKLNGIEKTYFDRATFESTVNCPSTPHPGVKW
                     ETTEKDGSNSTYEGLPLWIWVAMMDGGDLPAGHYRFNDSLARKGYRVVVVSADGFSAE
                     LDSGLMARNDRIILAYKKNGQYLSKNDGPLRLVGEDLPSKKHQVKQVVEIRLEGI"
     gene            complement(1988..3295)
                     /locus_tag="PLM83_02655"
     CDS             complement(1988..3295)
                     /locus_tag="PLM83_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOK70342.1"
                     /translation="MEEYKGRQGERREYGSRRERQIALIVFAAICIIAMAFIFLWPRF
                     GSRSYVFAVVGDNQGRNEVWNVVMDGVNSDRPDFVLHCGDMVASGTRAQYDDFLLGAE
                     KLRMPFYVVPGNHDVRGEGRECFAQLVNDPPYYSFEHEGYRFIGLDASEGSIDTEQTE
                     WLRNELSKDGHKFVFMHVPLYDPRPGGDHCFLDSEQAFELEKLFRESGVLAVFSGHVH
                     MFSHEDRDGVEYVTTGGAGALLYAPEEEGGFYHYVLVKVDGDQVDIEAKKVEVEFEEP
                     SLTLVGPAGEIELSLGELALMETLEVEAAFENKFENLGGKGLYVGVPVSRLVEMVGGM
                     VPGQTLVVTCADGYAQDYAYENLYPSELWAEIQGPMILAYEKDGATSPKWPESPRLVM
                     ASPDGVYSNEDCADTSMPGQGWNVYESAGARWARSVRRVEVKD"
     regulatory      complement(3435..3566)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(3758..4054)
                     /locus_tag="PLM83_02660"
     CDS             complement(3758..4054)
                     /locus_tag="PLM83_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70343.1"
                     /translation="MFERARSTAWTFQSRAHHINDGHVLRWLRLPMLMQSAPDCFTVH
                     MQKDEAARLGHNMTILGYLPQTSGAPPLIDHETSIVALESGVGMAISSVERDTN"
     gene            complement(4240..5229)
                     /locus_tag="PLM83_02665"
     CDS             complement(4240..5229)
                     /locus_tag="PLM83_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70344.1"
                     /translation="MPDRDQSGYRINPVWYGAVLLIAIIITLIALSVFGRSDMHPTDH
                     DETGVSESAFCWQADLPEGWRMVPNVDGGVRFVAEGFTVGGIERLPELPQGERDLTSL
                     LSENWVILSSMQVAVAAGWGAVFDISVPVHSDESASERGFETGESQSYIAAPTRPETH
                     CLVPLDGVTLDVWTRENTGFPPKEARDAAFSILQSLGLSGEYGEMQNRLPGEVMMIAA
                     ISDEAYPDGRIWGAFDVEATVRNVSDEDASDFKVLITKAVSNPAYLESDTEVDTSLDR
                     ISSLDPKADYILRIPGLEMSLPEKDYEVTVDIIGGGRPLKLGVRMAFPPGSED"
     gene            complement(5276..5521)
                     /locus_tag="PLM83_02670"
     CDS             complement(5276..5521)
                     /locus_tag="PLM83_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOK70345.1"
                     /translation="MRSTGIVRKVDGLGRIVIPMEVRKSLEIHEDDPVSISVDGETIV
                     LKRYRPFCTFCGNTEGIRTFKGKHVCGDCLSLIRKIG"
     gene            complement(5797..6804)
                     /locus_tag="PLM83_02675"
     CDS             complement(5797..6804)
                     /locus_tag="PLM83_02675"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOK70346.1"
                     /translation="MRRSDSWTLILGVVLMVIGSVLLLNNLGVTSVDLSYIISNFWPL
                     LIVGLGLYWVLKPDGSESGEVGEAERQATSEMPTSSVEPEPVDGEGCDECVEDAGEAD
                     ADSPRLTEVHTEESGLQEDVQSEATYDWGADTVDVADCQREGGPANGGEDEAELMRRR
                     SAMGWIIAILGLLILGSKTGRSPWSTGYAGGVFWAIVAIFIGLAMVRSSGEPAPEGKT
                     NWAVLSRIDHGQPGLELRSSSYMAILGSVVLNLSASRIPDEETRLDITAVLGCVHVVL
                     PPELSVKCEGTAVLGNVEAFGHSACGGLVNRDFVRSVPGGSSKSLHIKCRSILGSIDI
                     R"
     gene            complement(6826..8469)
                     /locus_tag="PLM83_02680"
     CDS             complement(6826..8469)
                     /locus_tag="PLM83_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HOK70347.1"
                     /translation="MADFGYNSIYPSRRSPVMGRRGAVATSHPLAAQAGIGMLAAGGN
                     AMDAALATAAALTVLEPTSCGLGGDAFAIVWDEGKMYGLNASGKAPMSLSADRLRAAG
                     MREVPKEGWASVTTPGVVSAWSALYDRFASMPFCEIMAPAIEYAAEGAPVPPVVAGYW
                     ADSEKRFGGRKGLYAEFERVFLPGGRAPRAGEIFRNPDLAETLRSIAGSYGRSFYRGS
                     LARAIADYSQDTGGYISEEDLREHAPQWVDPVSANYRGFDVWEIPPNGQGIVALMALN
                     IIEGYELESMDLMSPEVLHVVIEALKLAFADAHGYIGDPLCVDIPTDELLSKGYADRR
                     RRLIRHDRALERPAPGNPGRAHGRWEGSDGDTVYLCTADCYGMMVSYIQSNYMGVGSG
                     IVIPGTGISMQNRGAGFSLEEGHPNELAPGRRPFHTIIPSFITRDGIPIAAFGVMGGD
                     MQPQGHVQLVSAIIDGKLDPQTAIDRPRVRVLGSQGVAAEPSIGENVADALRRLGHEV
                     RLESEPAGFGGGQMIWRDPETGVLIAGSEPRKDGCAIAL"
     gene            complement(8497..9048)
                     /locus_tag="PLM83_02685"
     CDS             complement(8497..9048)
                     /locus_tag="PLM83_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOK70348.1"
                     /translation="MTIKIETPLSDSVVSSLRAGDEVSVSGVMYIARDAAHKRLVSLI
                     ESGEALPFDPRGQIIYYAGPTPPKPGQVIGSVGPTTSGRMDPYTIPLLELGLKGMIGK
                     GYRSAAVRKGLIEHRAVYFAAIGGAGVLAGQCMKSAKPIAYEELGPEALWRVEAEDFP
                     AFVVYDAYGGDLYEEGTSKYRVC"
     gene            complement(9070..>9708)
                     /locus_tag="PLM83_02690"
     CDS             complement(9070..>9708)
                     /locus_tag="PLM83_02690"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOK70349.1"
                     /translation="GIAVVFIELGQDVHVCGGDLNQAIDEGVRRGYSEAYLRKSVVHD
                     PLFGRVNTKDNTPAIVHLQMVPGCSLKITVVAKGGGAENMSGLAMLTPASGVEGVRNF
                     VVDQVRKAGPNACPPVIVGVGVGGNFETVAFLAKKALIRPVGERNPNPKYAALEEELL
                     REINGLGIGPQGFGGSVTALAVHIEAYPCHIASLPVAVNLNCHVAPHSTVIL"
BASE COUNT         2321 a         3018 c         2203 g         2166 t
ORIGIN      
        1 gccgactcgc ccacgcctgc gcgttcaaga atcgcagtca agggcactcc tgtgtaatcc
       61 gtgggaggta cgtgggtgac tgcacccttg agctcagcga cgaccgtgac attgtcgtct
      121 tcaaaatcaa taggtctgta attgtaagct tgagccaccg tgccctcgac acggcatgta
      181 ggcccattcc agaaaggttc gaagacgcat gcatagtagt acactgctcc gccggccaaa
      241 accagcacta gcgcaatggc aacgcccatg cggacaactg gcggtcgatg gacgctcctt
      301 cgctcttcat cgaccttaag tatgtgggcg gatgacacag catcaagcac gcctgcagtg
      361 aaatatcccc cgaacgctgc ccccgatgcg aaggcaaaag cggttatcag ccccacatag
      421 cccattgata cccctgaaaa gtacagggcc tggaatatga gcacgttgga agcagacgaa
      481 atcccgccag cgaggcacat tgccccaagg gaagggttac ttcccgaagg caacaacacg
      541 acatcaacaa gaattccctc aacagctgac acaattacaa ttggaaatcc gtgggtgctg
      601 ccggcaaaca tctcgactat ccctttaagg atcccagtaa gggatgccgc tcccgttcgc
      661 cccacaatcg ccctggcaaa cacgaaccag aatacatgga gtcctgccat ccattgaccg
      721 gcaccaaagg gcacaccgaa agccttgttc atgaggtttc ccagataacc aacgtaagtg
      781 ctgaggacgc ccccagcggc gctaatagcc gcaataagga cgagatctcg cgtactgaaa
      841 tagtaggctt tccgcattgg tcctggattc atccatgcca gccctccaca caatactgcc
      901 ctgccgaacg gttaccaata ttgcagatca acaaagtact gtgcgctata tgccctctaa
      961 cctgatttca actacctgct tcacttggtg tttcttcgaa ggcagatctt ctccgacaag
     1021 ccgcagcggg ccatcgttct tcgacagata ctgaccgttt ttcttgtagg cgagtattat
     1081 tcgatcgttt ctcgccatca ggcccgaatc gagttctgca gagaaaccgt ccgctgaaac
     1141 cactacaact ctgtaaccct tgcgtgcaag agaatcattg aagcggtaat gcccggcagg
     1201 caagtcccct ccgtccatca tggccaccca gatccagagc ggaagtccct cgtacgtcga
     1261 attgctaccg tctttttcag ttgtttccca cttgactccc ggatgaggag tgctaggaca
     1321 attgacggtg ctctcgaaag tggctcggtc aaaatacgtc ttctctattc cattaagctt
     1381 aatcatccaa tcgtccaccc cgggaacgac ttctatcttg gccaccgatt tcgcccagaa
     1441 atgtccgtca gtgatcgcgg ttttctcctt gtccataaag actattctcg ggagtttgtc
     1501 agcggagtcc cgatccgact cataggccaa atacatggca actccgccga tacgcaaagc
     1561 cctctgctga ttgtcatatg tgagaattcc tccgcaaacc tgagcatagc tgtaagtcat
     1621 cgcgtacccg tcacgagatg tgacgcgaat ggcttggcct tgatcgatgc ctccaacctt
     1681 cttgagtatt tcttccatcg gtacgccttt gtaaagcgca ggcccctcta ttcgccctgt
     1741 agttctaagg aaagcagctt tagccgacac aggtttcatc ctgccaaaat cggtctctgt
     1801 caaagtgacg cttctctgtc ccgatactac ttcgaccttc cagttgttct gggcctgtgc
     1861 aggcatccag aacacgggca tcgccataaa cccgaccaca aaagcgagaa acacgaccaa
     1921 cgctagacgg ttcttcctca tacaataacc tcctccattt tcggttacca acacacgcaa
     1981 acgaaggcta atccttgacc tcaacacgtc ttacactccg cgcccatctg gcgccggcag
     2041 actcatacac attccacccc tgcccgggca tggatgtgtc tgcacagtcc tcattgctat
     2101 ataccccgtc cggagacgcc ataacaagcc tcggagactc cggccacttt ggcgaagtgg
     2161 cgccgtcctt ttcgtaagcc aaaatcatcg ggccctgtat ctctgcccac agctcagaag
     2221 ggtagagatt ctcgtatgca taatcctgag catatccatc agcacatgtc accacgaggg
     2281 tctgtcctgg aaccatcccc ccgaccattt cgacgagacg gcttacagga acccccacgt
     2341 aaaggccctt cccaccaagg ttctcgaatt tgttctcgaa agcggcctca acctccaagg
     2401 tttccatcag cgccagctcg ccgaggctca actcgatctc cccagcgggg cctaccaacg
     2461 tcaaagaagg ctcttcaaac tccacttcca ctttcttggc ttctatatcc acctggtcac
     2521 cgtcaacctt gaccaagaca tagtggtaga atccgccctc ttcttccggg gcgtataaga
     2581 gagctcccgc gccgcctgtt gtcacatact cgaccccatc gcgatcttca tggctaaaca
     2641 tgtggacatg cccactgaac acggccaaaa caccgctctc tctgaacagc ttctccagct
     2701 cgaacgcctg ctcggaatcg aggaagcagt gatctcctcc aggacgaggg tcatacagcg
     2761 gcacatgcat gaagacgaat ttgtggccat ccttggacag ctcgttcctg agccactccg
     2821 tttgctccgt gtcgatgctc ccctcggagg catctaagcc tatgaacctg tatccttcat
     2881 gttcgaacga gtagtacggc ggatcgttga cgagctgagc aaagcactct cgtccttctc
     2941 cgcgaacatc gtgatttcca ggcacaacgt aaaaaggcat tctgagcttc tccgcaccga
     3001 gcaagaaatc atcgtactga gcccttgtgc ccgatgcaac catgtcgcca caatgaagca
     3061 cgaaatcagg acggtcggag tttaccccgt ccatcacgac attccagact tcgttccgcc
     3121 cctggttgtc accgacgacg gcaaagacat agcttcggct accaaaccga ggccacaaga
     3181 aaatgaatgc catagctata atgcagatgg cggcgaatac gatgagtgcg atctgtctct
     3241 ccctacggct cccatattct ctgcgctcac cttgccgtcc tttgtattcc tccaccttcc
     3301 cacctccgct tacagccgtt ctgtaggggt cttgcaatgc cccaaaagtc tcctggaacc
     3361 gcagcaggca aatgtcgata gtttaatgac agaatgggcc gggcgccaca gaaaggtggc
     3421 agaccccggc ccgagaatcc ggtgtttgct ctcctttcca aatcgggaag cacacccatt
     3481 tccagatacg ccctatcggc ccacacgccg cagctccgtt tcaggcctga gcccaaggcc
     3541 tgtgggctcg gacaagcaat ctcattgact attcgacggc gtaaccacac gtccctacaa
     3601 tcgcaacgct tcttgcctct gcatcgattc acttctgcct tcatggatag aaaaacacgc
     3661 accctgcaaa agcccttcaa tgaaatgccc tgcgccatag gccttcacag acttcttcta
     3721 agcaacaatg ccattgtcgg gccacaaatg aattttccta attggtgtcg cgctcaaccg
     3781 aactgatcgc catgcctaca ccagattcca aggcaacaat ggatgtctcg tggtcaataa
     3841 gaggtggcgc acccgaagtc tgaggcagat accccaggat ggtcatgttg tggcccagcc
     3901 tagcagcttc gtctttctgc atgtggaccg taaaacagtc tggcgcagat tgcatcagca
     3961 taggtagccg cagccatctt aacacgtggc cgtcattgat gtgatgagct ctggattgga
     4021 aggtccacgc agtagaacgc gcccgctcga acaagattgc tgcctcacct tacttgagcg
     4081 ggcgaccttt ttcagtctga agtagtggcg cagatcacgt cggaaaatgc gtccgccttt
     4141 ccaggtgccc cctgtctatc gcctgcacca tattgaaact cgcgttaccg caaagctgga
     4201 tcttgctagt ttgaaactgg agtgctgatc gaaggctatt cagtcttctg aacccggcgg
     4261 aaaagccata cgcaccccga gcttgagtgg gcgccctccg ccgatgatat cgacagtcac
     4321 ctcgtaatcc ttctccggaa gactcatctc cagacccgga attctcagga tgtaatctgc
     4381 tttcgggtca agagaagaga tcctgtccag gctagtgtca acctccgtgt ctgactcgag
     4441 ataagcagga ttcgagactg cctttgttat caacacctta aaatcgcttg catcttcatc
     4501 agatacattg cgaactgttg cctctacatc aaaggctccc cagatccgtc catctgggta
     4561 cgcttcatcc gatatggcgg cgatcatcat cacttctccg gggaggcgat tctgcatctc
     4621 gccatattct ccacttagtc ctaagctttg cagaatggaa aaggcggcat ctctggcctc
     4681 tttaggagga aagccggtgt tctccctggt ccatacatct agcgttacac catcgagcgg
     4741 aacaaggcag tgggtttcag gcctggtagg cgcggctatg tacgattgag attccccagt
     4801 ttcaaagccg cgctcagaag ctgattcatc tgagtgcacc ggcactgata tatcgaaaac
     4861 agctccccat ccggccgcaa cagccacctg catggaggag agtatcaccc agttttcaga
     4921 gagcaagctc gtaaggtccc gttctccctg aggcagctct gggagcctct ctattccacc
     4981 cactgtgaat ccttccgcta caaaccttac tcctccatcc acgttcggga ccatccgcca
     5041 accttcggga agatccgctt gccaacagaa cgcagattca gaaacgccag tctcatcgtg
     5101 atccgtcgga tgcatgtccg aacgtccgaa caccgacagc gcaattaagg taattatgat
     5161 tgcaatgagc aaaactgcgc cgtaccagac tggattaatt cgatatccgc tctgatcacg
     5221 atctggcata aagatcctcc cttcatgacc attctacgcc atgtgctaaa ctccgctagc
     5281 ctatcttcct tatcaatgat aggcaatcgc cacacacatg cttccccttg aacgtcctta
     5341 ttccctcagt attgccgcag aacgtgcaga agggcctgta cctcttgagc actatggtct
     5401 ccccatcaac actgatggac acagggtcat cttcgtgaat ctccagcgac tttcggactt
     5461 ccataggtat cacgattcgc cccaacccat caacctttct tacaatgcct gtagaacgca
     5521 attgtaatcc cccctatact ggctagcttg cgccatatgc gcgcaggtcg acaaagcctg
     5581 gatcattggg ttcgccgcgt ctttacgcat gcatataccc catctcgaac aatttcgcta
     5641 attctctcgt gactcctccc gagaggatga gaaaacatgc aacagtccat cgcaatttag
     5701 cggcggcgtc gatatctcaa accccggacc tcgtcacaac aattccaggc ccccatcgtt
     5761 ctccggctgt cagcacagga gccctacaat agggcgttat cggatatcta tagaacccag
     5821 tattgaacgg cacttgatat gaagcgattt gctcgaaccg cccggaacac tgcgcacgaa
     5881 atcacggttg accaatccac cgcaggcaga atgtccaaat gcctccacat tgcccaaaac
     5941 agccgttcct tcacatttga cagacagctc aggcgggagc accacatgta cgcagccgag
     6001 aactgctgtg atgtcgaggc gtgtttcttc gtcaggaatc cgtgaagccg acaaattcag
     6061 cacaacactg ccaaggatcg ccatgtacga actgctccgc agctccaaac ctggttgccc
     6121 atgatcgatt ctgctcaaca ccgcccagtt tgtcttgcct tcaggagcag gttcacccga
     6181 tgatctcacc attgccaggc cgatgaatat ggcaacaatc gcccagaaca ctccgccggc
     6241 ataccccgtg ctccagggag aacgtcccgt tttgctgcct aagatgagca agccgagtat
     6301 ggctatgatc catcccatgg cggaacgacg cctcatcagc tcagcctcat cctcgcctcc
     6361 atttgcaggc ccaccttctc gctggcaatc agcaacgtcg accgtatctg cgccccagtc
     6421 atacgtagct tcgctctgca catcttcttg aagtccactc tcctcagtat gcacctctgt
     6481 caggcgcggg gagtcagcat cagcctcgcc agcatcttca acgcattcat cacatccctc
     6541 tccgtccaca ggctcaggtt ctaccgacga ggtaggcatc tccgatgttg cctgcctctc
     6601 tgcctcgcca acctcgccag attcgcttcc atcaggcttc aacacccagt atagtcccag
     6661 gccaacaatg agcaaaggcc agaaattgct gataatgtag ctaagatcaa cactggtaac
     6721 gcccaagtta ttgagcaaga gcacagatcc aataaccatg agcacgactc cgagaataag
     6781 cgtccatgaa tcagatcttc tcatttcacg cccccccatt cccgatcata gcgcaatcgc
     6841 gcatccatcc tttcgcggtt cgctccctgc gatcaatacc cctgtttcgg ggtcacgcca
     6901 gatcatctgg ccaccgccga atccagcagg ctcactctca agcctaacct catgccccaa
     6961 gcgacgtaac gcatccgcaa cattctcacc gatgctcggc tctgcggcta caccttgact
     7021 gcccagaacg cgcacgcggg ggcggtctat ggccgtctgc gggtcaagct tgccatcgat
     7081 gatagcagaa actagctgga catgtccttg aggctgcatg tctcccccca tcactccaaa
     7141 agcagcaata ggaataccat ctcgagttat aaaggacggg attatggtgt gaaatggcct
     7201 cctgccagga gcaagctcat tgggatgccc ttcttccaat gagaaaccag ctccgcgatt
     7261 ctgcatagag attccagttc ccggaattac aatgccggag ccaaccccca tgtaatttga
     7321 ttgtatgtaa gagaccatca tcccgtaaca atccgctgtg caaagataga ctgtatcccc
     7381 atctgaaccc tcccatcgtc catgagctct tccgggattg ccaggggctg gacgttcaag
     7441 cgccctatca tgacggatca gtctccgtct gcggtcagca tatccttttg atagcagttc
     7501 atctgtggga atatccacgc aaagcggatc acctatatag ccatgggcat cagcaaaagc
     7561 cagcttcagg gcttcaatga caacatgaag cacttcagga gacatcaggt ccatagattc
     7621 caattcataa ccttcaatga tgttgagagc catcagtgcc acaatgcctt ggccattggg
     7681 aggaatctcc catacatcga atccccggta gtttgcggac acaggatcaa cccattgtgg
     7741 agcatgttca cgcaaatcct cttcagatat gtagcctcca gtatcctgcg agtaatcggc
     7801 aatcgcccgc gccaagctgc ccctgtaaaa ggatcgcccg tatgaacctg caatcgatct
     7861 taatgtctct gccagatcgg ggtttctgaa gatctccccc gcacggggcg ctcgcccgcc
     7921 cggcagaaaa accctttcga actcggcgta aagtcctttg cgcccgccaa atcgcttttc
     7981 ggaatctgcc cagtaacctg ccacgacagg tgggacaggc gcgccttcag cggcgtattc
     8041 aatggccggc gccatgattt cgcaaaatgg catggaagca aagcgatcat acagcgcact
     8101 ccacgctgaa accacgccag gcgtagtgac agatgcccaa ccctccttgg gcacctctct
     8161 cattccagcc gctcgaagcc ggtctgctga aagagacatc ggggccttgc cggacgcgtt
     8221 cagcccgtac atcttccctt catcccagac tatagcaaat gcatccccgc ccagtccgca
     8281 cgatgtaggc tcaagaactg tcaaagccgc tgccgtcgcc aaagccgcat ccatagcgtt
     8341 gccccctgcg gcaagcattc cgatgcctgc ctgagcagcc aatggatggc tcgtggccac
     8401 cgctccgcga cgtcccatta cagggctccg ccgagacgga taaatcgaat tatagccgaa
     8461 atcagccatc ctgtatcccc cttcatcgag gcagcgctag cacacccgat atttgctagt
     8521 cccttcttca tagaggtccc cgccatatgc gtcgtagaca acgaatgcag gaaagtcctc
     8581 cgcctccacg cgccacagag cctcagggcc aagttcctcg taggctatgg gtttggcaga
     8641 cttcatgcac tgccctgcaa gtacccctgc tcctcctatt gcagcaaaat agactgcacg
     8701 atgctctatt agacccttgc gaacagcagc ggagcggtat cccttcccta tcatgccttt
     8761 taggcccagc tccagcaagg gaatggtgta tgggtccata cgtccgctcg tagtaggccc
     8821 cacagaacct atcacctgcc caggtttggg cggcgtaggg cctgcatagt aaattatctg
     8881 gcctctagga tcaaatggga gagcctcgcc tgactcgatg agcgaaacca aacgtttgtg
     8941 cgctgcatcg cgagcaatgt acataactcc gcttacgcta acctcgtccc ctgccctgag
     9001 agaggagacg acactatccg aaagcggagt ctcaatttta atggtcatct aattaatccc
     9061 tccggcaaat tacagtatta ctgtggagtg cggcgccacg tgacagttga gattgaccgc
     9121 tacaggaagg cttgctatgt ggcaagggta cgcctctata tggactgcaa gggcggtaac
     9181 ggatcctcca aatccttgcg gcccaatccc cagcccgttt atctcgcgca acaattcctc
     9241 ttccaatgct gcatactttg gattcggatt ccgttcccct acgggccgaa tcaaagcctt
     9301 cttggccaga aatgccactg tctcaaagtt gccgcctaca ccaactccta caatcacagg
     9361 aggacatgca ttagggccgg ctttccgaac ctggtccaca acgaaattgc ggacaccttc
     9421 tacgccagat gccggagtaa gcatggcaag tccgctcatg ttctcagccc cgccaccctt
     9481 ggcaactacg gtaatcttga gggagcatcc cgggaccatc tgaagatgga ctatggcagg
     9541 ggtattgtcc ttagtattga ctctgccaaa cagggggtcg tgcaccaccg attttctgag
     9601 atatgcctcc gaatatccgc gtctgactcc ttcgtcgatc gcctggttta ggtcgccccc
     9661 acacacgtgc acatcctgcc caagctcgat gaagactacg gcaatgcc
//
LOCUS       DAOSAI010000041         9614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4652_length_9614_cov_4.101057, whole genome shotgun sequence.
ACCESSION   DAOSAI010000041 DAOSAI010000000
VERSION     DAOSAI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4652_length_9614_cov_4.101057"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1093
                     /locus_tag="PLM83_02695"
     CDS             140..1093
                     /locus_tag="PLM83_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HOK70350.1"
                     /translation="MLAPRPVIDILRRYGMTFILLAMIAALSFMNPRFFSVSNFLTIM
                     RQVAINGALAAGMTMVILTGGIDLSVGGNVALAGCFAAGALQSTGNPWIAIVVALGVG
                     LMIGMVNGFFVSYAKLPPFIVTLATLALARGFTLVYTEGRPILVDSPIFEVIGGGFLA
                     GVPVPVLIVAVVYLIVGFVLNRTKFGRLVYAIGGNEQACRLSGVNVERTKMLVYVWCG
                     LLAGLSSVILSARLLSAQPTAGTGYELDAIAAVILGGTSLSGGEGGVGGTLVGAFIIG
                     VLANGLNLLNVSPFYQDVAKGAVILAAVLMDKAFHSTANAR"
     gene            1121..1540
                     /gene="rbsD"
                     /locus_tag="PLM83_02700"
     CDS             1121..1540
                     /gene="rbsD"
                     /locus_tag="PLM83_02700"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HOK70351.1"
                     /translation="MKKSGIINPDLCRAIASLGHTDSIVICDAGLPIPQGVERIDLSL
                     VRGVPSFDQVFQEVVRELQVERAIVAEEALRENPHVIRVITEALGEGRVEFVPHLRLK
                     ELTEYAKTVVRTAEFRPYANVILYSGVEGLFEDGLPD"
     gene            1647..2546
                     /gene="rbsB"
                     /locus_tag="PLM83_02705"
     CDS             1647..2546
                     /gene="rbsB"
                     /locus_tag="PLM83_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HOK70352.1"
                     /translation="MRSNRILSVFLCVLMIGSMFASMASAAAPKIGLAISTLNNPFFV
                     DLRDGALEEAKKAGLGITVADAQNDPNRQLSQVENFIQQGVNIILVNPCDSAAIVSAI
                     KAANRAKIPVITVDRGADGGEVICHIASDNVQGGQMAGEYLAKLIGYKGKVVEIEGIP
                     GASAARDRGKGFNEVMKKYPNIKIVARQEAGFDRAKGMTVMENILQAQPDIDGVFCHN
                     DEMALGALRAIEAAGRLSAIKIVGFDATDDAIKAVQDGKLVATVAQKPRAMGSMAVTV
                     AKRVLDGAKVDAFIPVPLELVAK"
     gene            3825..4559
                     /gene="cysE"
                     /locus_tag="PLM83_02710"
     CDS             3825..4559
                     /gene="cysE"
                     /locus_tag="PLM83_02710"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOK70353.1"
                     /translation="MLKDIGSYIDAAMARDPAARNRLEVLLLYPGIHALIWHKLSHWL
                     YVNKAFGLARLTSQLVRFFTGIEIHPGATIGRGVFIDHGMGVVIGETAEVGDDVTIYQ
                     GVTLGGTGKETGKRHPTLEEGVVVSAGAIVLGSITVGSNSKIGAGAVVIRNVPPDSTV
                     VGVPGRVVARAGVRVREADLDHGDLPDPIGRALDLMALRVERLEAELETLRRISRSGQ
                     AVGYGGAPCEPLPESEEEEEAIEYCI"
     gene            4543..5469
                     /gene="cysK"
                     /locus_tag="PLM83_02715"
     CDS             4543..5469
                     /gene="cysK"
                     /locus_tag="PLM83_02715"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOK70354.1"
                     /translation="MSIASDVTGLIGHTPMVRLSRFGGGGRGEIIAKVESANPGGSVK
                     DRAALYMVDAAERAGVLAPGGTIIEPTSGNTGVGLAMIAAARGYRIVLVMPDSMSIER
                     QKLLAAYGAELVLTPGSHGMAGAVARARELAAEHSDWFMPLQFENPENCRAHEETTAR
                     EILADVEGRLDAFVAGIGTGGTVTGVGRALKREIPSVLVVGVEPQESAVISGGEPGLH
                     GIQGIGAGFVPSVLEMSILDEVISVSSDDAYRATRALAACEGLLVGISSGAVAVAALE
                     VARRLGEASRVVVLFPDTGERYLSVPGLWRVG"
     gene            5784..6716
                     /locus_tag="PLM83_02720"
     CDS             5784..6716
                     /locus_tag="PLM83_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK70355.1"
                     /translation="MNRRTNDDGSSALQKGFDAIARQNWFEAEEVFADFVDSEDASVE
                     AWLGWAIVQQSMGFRDEAASAVRQALAVLADDELEIQVLGELYSSIGLEQDALPFLLR
                     AADAASGDLDELERIAGLMLKIGYVRWGVDIAERVLAVDPERPEAWLIIGLSRFIDSR
                     WDEAVKAFEESLVRNPAHPMTHLSLGAARLEQNLLEQALEHLDFVIALPRNADEDLRY
                     ACSAMIRRARVLLLMGRRAEAVDTISEAAEIAGDSPTDLFDIGLFYLDDMGSANEALP
                     FLRKAAALDPYDSFKQATLAHAQLEAGEGVDSPR"
     gene            complement(6745..7035)
                     /locus_tag="PLM83_02725"
     CDS             complement(6745..7035)
                     /locus_tag="PLM83_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK70356.1"
                     /translation="MFDEAGFVVRATLATWTATDDGDDEPGKFKIRAIMPGNYSLRAT
                     AEGYIPAEVSGLVVHEGADLELEEIWNEDCIRITGGEFDEEGRIVLLTHANR"
     gene            complement(7028..7600)
                     /locus_tag="PLM83_02730"
     CDS             complement(7028..7600)
                     /locus_tag="PLM83_02730"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HOK70357.1"
                     /translation="MQTGKWLPSTDTLAICLADAPIDTCEDVNIGIKKVFVKPFNYSD
                     QIIVAALPGPARVNLLDLRINEFVMTASISAGIYEDTCLLLDAEGYVKLRGEDFAYPL
                     VAGDAEELDEDDDEQAIEIEYPFEVCSSRVTEILIDFDLAKVLQPNELGNGYVLDPDE
                     IEAVDKLDVGEIEGEVVATDHRQASCCRVV"
     gene            8163..9212
                     /locus_tag="PLM83_02735"
     CDS             8163..9212
                     /locus_tag="PLM83_02735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOK70358.1"
                     /translation="MSRTRIYELDVFRAICTITVLLGHVSSYPVGVLSTDSRFYGLYL
                     GVNSFCRFAIQAFLFLSAAVLVHSYADRWQGPESALAMWRKRLIDPGIPYVVWSVIYT
                     LLAIRRGRHIDFPTFLRLLATGKAWDHLYFIVLIFQFYLLFPLLLRALRSLSCRPVLW
                     LGIGALIQAGFHMWDQRVFAIPNRASWAARFGFYLFAGMLAGLDFDVLQDWTRRNRWA
                     IAAVWAVSAVLNCSVSAAIRLGTPHRLSGYAELIVNVYAVASCLFFLAVSQWVTASNG
                     WPMRAAMGLSNASFGIYLSHPLGLAMWRRLTATGNPILFHLSVWAGAVVVLALSWAVT
                     LWLKRFKFGWTLVGK"
BASE COUNT         2019 a         2266 c         3017 g         2312 t
ORIGIN      
        1 ctgatttcct ctgagatacc cgaggttgtt ggaatgagcg atagagtttt ggtgttgcat
       61 tcgggcagag tcgtaggcga actgatgggc gatgagtgca cacaggaagc ggtattgaga
      121 ttggcgactg ggggtagtgt tgttggcgcc taggcctgtt atcgatattc tcagacgtta
      181 cgggatgact ttcatacttt tggccatgat cgctgccctt tcattcatga atccgcggtt
      241 cttctctgtt tcgaactttt tgacaataat gaggcaggtg gcgatcaacg gagcactggc
      301 ggcaggcatg accatggtga tacttacggg agggatagac ctttcggtgg gcggaaatgt
      361 ggccttagcg gggtgttttg cagcaggcgc tctgcaatcc acaggcaacc cttggattgc
      421 gattgtcgtg gcgcttgggg tggggctcat gatcggcatg gtcaatggct tctttgtttc
      481 ttatgcgaag ctcccgccat ttattgttac tttggctacg ttggcgcttg ccagaggttt
      541 tacgttggtg tacacggagg gcaggccaat actggtcgat tctccaatct tcgaagtgat
      601 aggcggcggt ttcttggctg gcgtgccagt gccagttctt atcgtggcag tggtctattt
      661 gatagtcggc ttcgtgttga accggacgaa gtttggacgg cttgtttacg ctataggcgg
      721 aaatgaacag gcctgcaggc tgtcgggagt aaacgttgag agaaccaaga tgttagttta
      781 cgtatggtgc ggccttctgg cagggctgtc atcggtgatt ctatcagcga ggctgctttc
      841 agcccagccc actgcgggga cgggttacga gcttgatgcc attgctgcag ttatccttgg
      901 gggcacgagc ttgtccgggg gtgaaggcgg cgtaggaggg acgcttgtcg gcgctttcat
      961 tattggagtg ttggcgaacg gactcaatct gctcaatgtg tctccgttct accaggatgt
     1021 ggccaaaggc gctgtgattc tggcggctgt gctcatggac aaggcctttc acagcactgc
     1081 caatgccagg tgatagacga aggatggagg acgatagaga atgaagaaat ccggcataat
     1141 caaccccgat ttgtgtaggg ctatagccag ccttgggcat acagactcaa ttgtcatctg
     1201 cgatgctgga ctgccgattc cgcaaggcgt tgagaggata gatttgtcat tagtgcgcgg
     1261 tgtgccgtct tttgatcaag tgttccagga ggttgttcgg gagttgcagg ttgaacgagc
     1321 gatagtggca gaggaagcgc tgagggaaaa tccgcacgtg atcagagtta taacggaagc
     1381 tttgggcgaa ggtcgagtcg agttcgtgcc ccatcttagg ctgaaggaac tgacggagta
     1441 cgcgaagacg gttgtcagga ctgctgagtt taggccctat gccaatgtca tcttatactc
     1501 gggtgttgag ggcctgttcg aggacggatt gccggattga ctgggaggtg agcataaagg
     1561 tcatacacgg gtcataccgg gcatactgtg gatgtgttgg gagtttatag gagactatgg
     1621 cactatgata aggggaggcg ctttgaatga gatccaatag gatactttcg gtctttctgt
     1681 gtgtgctgat gatagggagc atgtttgcgt caatggcgag cgcagctgct ccaaagattg
     1741 gtcttgcgat ttcaactctc aacaacccgt tctttgtgga tctgagagat ggagctttgg
     1801 aggaggccaa gaaggctggg ctgggcatta ctgtcgctga tgctcagaat gaccccaaca
     1861 gacagctatc gcaggtggaa aacttcattc agcaaggggt caacatcatt ttggtcaacc
     1921 cttgcgattc tgcagccata gtttcggcca taaaggcggc aaatcgagcg aagataccgg
     1981 tgatcaccgt tgatcgcggc gcagatggcg gtgaagtcat ctgccatatt gcgtccgata
     2041 acgtgcaggg cggccagatg gccggcgaat acttggcaaa acttatcggc tacaagggaa
     2101 aggtagtaga gatagagggc atacctggcg cttctgctgc gcgcgaccgg gggaaaggct
     2161 tcaatgaggt gatgaagaaa tacccgaaca tcaagattgt cgcgagacaa gaagcgggat
     2221 ttgacagggc gaagggcatg acagtcatgg agaacattct ccaggcccag ccggacatag
     2281 atggggtgtt ctgccacaac gacgagatgg cgttgggcgc gctcagggcc atagaggctg
     2341 ctggcaggct gtctgccatt aagatagtgg gatttgacgc tacggacgat gccataaaag
     2401 cagtgcaaga cggcaagttg gttgccactg tagcccagaa gcctcgcgca atggggagca
     2461 tggcagtgac agtggcaaaa cgcgtgcttg atggggctaa ggtggatgcc ttcatacctg
     2521 ttccgctgga gctcgtggct aagtagtcat cgagttgcat agagaatagg ttgtagactg
     2581 tggagcggtt gtggccaagg ccacttggct gcagccgctt catgtcgcct acctaacgcc
     2641 tataggcgct acccaagtca gttgttggct ggcgcgcaag gcattggttt gatctgcatg
     2701 tgctatgtta gcgtcgcagc gtcaaagact gtcatccgga gacaatgcta agtaggtctc
     2761 gatacgtttc tgtgcgatgt gttacctgcg gttcaaacct ggaccgtttg atctggacat
     2821 gatgctgctt gtgattgctc aggtatgtcc aaaaaagcgt ttgagtgttc cccggggcag
     2881 ctgaggccct tgcgccggat agcaatcgag ttcaacagtc gcgcaaacag cgccgcaagg
     2941 ctgggctgcc ctttgagacc cggacaagaa gtgtgtggcg ggggagaaag ggggtgtcaa
     3001 cggacttggc caacttcaag tcacatcgcg atcgcaagtg ttcaagtctt agacacatcc
     3061 cattgatgcg aaccagcgga taccttgtta tggatatgct cactcgcaaa aaacggtcca
     3121 acgcgtgtca acaggctgtt cccgagctaa gtatacgtgt gcaggcaaaa tccaagacca
     3181 agctgtgccg attacttgac ttgtctgaag taccgagaaa gccgcctatc ggcaagaatt
     3241 tttccaaagc tacttgggcg ttgtcttgag gcagggatgg tgggattaga atggaaggct
     3301 gaacgttcag atgggcccga cgggatcatg tgctaggcca ataatttgga atactgggct
     3361 tttggctatc ttgacagcag acgtcggaag acatatgcta tatcccaaac agcaaagatg
     3421 caagcggcta tgagagggag gtcgggggcg gactgcccag agagagggaa cgtcaccggg
     3481 tgcaagcgtt cccgggcaag cgcttcttga gtgcgccctt gagcctggcg ctgaaactgt
     3541 ctgcaggcag cgggtgggag taggtggcca cgggtgcgcc cgttatcgcg tgttccccat
     3601 ttcgagcaag gctttgcctg ccaaagtggg gagcctgagc tgcggttggc cgagcgcgca
     3661 ttgtcggata tcggccatcc gtgagcagag tgggaccacg gacagatgag ccgtctctgt
     3721 aaagaggcgg cttttgtgtt atataggcct tttcctggca gggtcgcctc tgcagactcg
     3781 ttgctgttta acacattacg gcacagccga ggagttgcat gaaaatgctg aaggatattg
     3841 gttcatacat cgatgctgct atggcgcgtg atccggcggc cagaaacagg ttggaagtgt
     3901 tgcttttgta cccgggcatt cacgctttga tatggcataa gttgagccat tggctatacg
     3961 tgaacaaggc attcgggctt gccaggctca cttcacagct ggttcgcttc tttacgggga
     4021 tcgagataca cccgggagcg accataggcc gaggtgtctt tatcgaccac ggcatggggg
     4081 tggttatcgg agagactgct gaggtcggag atgacgtcac catctaccag ggcgttacat
     4141 tgggaggtac cgggaaggag accggaaagc gccaccccac tttggaagaa ggggtagttg
     4201 tgtcggcagg cgctatagtg cttggttcga taacagtggg gagcaattcg aagatcggtg
     4261 ctggcgctgt cgttattcgt aatgttccac cagattcgac ggtagtgggc gttccaggcc
     4321 gtgttgttgc aagagcaggc gtgagggttc gcgaggccga tctcgatcat ggcgatttgc
     4381 cagaccccat aggacgcgct ctcgacctca tggcgctcag ggtggaaagg ttggaggcag
     4441 agcttgagac attgcgcaga attagccgtt ctgggcaggc cgtgggatac ggaggggctc
     4501 catgtgagcc gctgcctgaa tcggaagagg aggaagaggc aattgagtat tgcatctgat
     4561 gtgaccggat tgatcggcca cacgcctatg gtgcggctta gccggtttgg cgggggcggt
     4621 cggggcgaga ttattgctaa ggtagaatcg gccaaccccg gagggagcgt gaaagacagg
     4681 gctgcacttt acatggtaga tgctgcggag cgtgcaggcg ttctggcgcc gggaggcaca
     4741 ataatcgagc cgacgtctgg taatacgggg gtggggcttg ccatgattgc tgcggccaga
     4801 ggctacagaa tagtccttgt tatgcccgat tctatgagca ttgaaaggca gaagcttctt
     4861 gctgcatacg gggcggagct tgtgctgacc ccaggctcac atggcatggc gggcgctgtg
     4921 gccagagctc gcgaattggc ggccgaacac tccgactggt ttatgccgct gcaatttgaa
     4981 aatcccgaaa actgccgggc tcatgaggaa acaacggccc gagagatact tgcagacgtc
     5041 gaaggtcggc tagacgcatt cgttgccgga attggaaccg gcggaactgt gaccggtgtg
     5101 gggcgggcat taaaacgtga aatcccgtca gtactcgtgg tgggagttga gccgcaggag
     5161 tcagctgtta tatccggcgg cgagcccggc ctccacggca tacagggcat tggagcgggt
     5221 tttgttccca gcgtgctgga gatgtcgatc ctcgatgaag taatctcggt tagctctgac
     5281 gatgcctatc gcgcgactcg ggcacttgcg gcctgcgaag ggttgttggt gggaatatcc
     5341 tctggagctg tggccgtggc cgcgcttgag gtagcgcgca gactgggaga ggcgagccgg
     5401 gttgttgtct tattcccaga cactggcgag cggtacctga gtgttcccgg attatggcga
     5461 gttggatgag ctagccggtt ctgaatgcga gacctttggg atctgttgtt taggagaggt
     5521 tgagcaaaca aggcagtcaa gccgtgcttt tcgcaccgct tggcccatag atcaaagtgg
     5581 acaatgacgc gattgtcgga ggaattcaat gcctcctaat cgccacttga aggagaatca
     5641 tgttctgcgg ggaaatttag ataggcccga tttcgatgcc cggttgtggg gacgaacagg
     5701 ttcggtgtcc gaaaagaact gctgtgcccg ctcagtctgg agatgggggt aggcagcgga
     5761 tagcggccga ggaggagaca acagtgaaca ggcgcacgaa tgatgatggc agcagcgccc
     5821 tccagaaggg ctttgatgct attgcccggc agaactggtt tgaagcagaa gaggtttttg
     5881 cagacttcgt ggattccgaa gatgctagcg ttgaagcatg gttgggctgg gccattgtcc
     5941 agcagagcat gggatttagg gacgaagccg catctgcagt caggcaggcg ttggctgtcc
     6001 ttgccgatga cgagttggaa atacaggtgc tgggagaatt gtattccagc atcggcctgg
     6061 aacaagatgc gctcccattt ttgctcaggg ccgcggacgc cgcctccggc gatctggatg
     6121 agctggagcg cattgccggt ttgatgctga agatcggcta cgtgagatgg ggagttgaca
     6181 tagccgagcg tgttctggcg gttgaccctg aaagaccgga ggcttggctt attatcggcc
     6241 ttagccggtt catcgattcc cgctgggatg aggctgtgaa ggctttcgag gagagcctcg
     6301 tgagaaatcc cgctcatccc atgacccacc tctccctcgg tgcggcaagg ctggaacaga
     6361 accttttgga gcaggctttg gaacatctcg attttgtcat agccctgccg aggaatgccg
     6421 acgaagactt gaggtacgca tgttcagcca tgatcaggcg tgctagagtc ctgctgttga
     6481 tgggccgcag agcggaagct gttgatacta tttccgaggc cgcggagatt gcaggggaca
     6541 gtcctactga cttattcgac ataggccttt tctacttgga tgacatgggc tctgccaatg
     6601 aggctctgcc gttccttcgc aaagctgcag cccttgatcc atatgattca ttcaagcagg
     6661 ctacccttgc ccatgcgcag ctggaagcgg gcgagggtgt tgacagcccc agataggcat
     6721 ttgccgttcc aatgtccgca gacattatcg gttcgcatgg gttaggagaa cgatccgtcc
     6781 ttcctcgtcg aactctccgc ctgtgatgcg tatgcaatct tcattccata tctcttccag
     6841 ctcgagatct gcaccttcgt gcacgacgag tccggatact tcggcgggaa tatagccttc
     6901 cgctgtggcc cgaaggctgt aattgcctgg catgatggct ctgatcttga acttgccggg
     6961 ctcgtcatca ccatcatccg tcgctgtcca agtggctaag gttgcgcgaa cgacgaatcc
     7021 agcttcatca aacaactcta caacaactgg cctgtcgatg atcggtcgcc acgacctcgc
     7081 cttcgatttc tccgacgtcg agcttgtcta cggcttcaat ttcatcagga tctagcacgt
     7141 aaccgttgcc gagttcgttt ggctgaagaa ctttggcgag gtcaaaatcg ataagaatct
     7201 cggttaccct gctgctgcaa acctcaaatg gatactcgat ttctatggct tgttcgtcat
     7261 catcctcatc aagctcttct gcatcaccgg cgacaagggg atatgcaaaa tcctctcctc
     7321 gaagcttcac atagccttct gcatccagca ataggcaagt gtcctcgtaa atgccagccg
     7381 aaatagatgc ggtcatcacg aactcgttga tcctcagatc gagcagattg actctggcgg
     7441 ggcctggcaa tgctgctact ataatctggt ccgagtaatt gaaaggcttc acgaagacct
     7501 ttttgattcc gatgttcacg tcttcgcaag tgtctatggg agcgtctgca aggcagatag
     7561 ccagggtgtc ggtggatgga agccatttgc cagtctgcat cctgggagca atgccaggca
     7621 accgcagaca attgtgagta gcaacatagc cgcaatcgag tgttgtttca atcgaaacgc
     7681 ctccttgatc agcatgatac ctgcccattg tttaatgcga agggaagagg atcgctgatt
     7741 ctgctgttgc cgcgttggca ggtggcttag ggcatgatgc agaattcgtc ctgagggatg
     7801 gacgaacgtg agggcttttg cgtatcaagt tgccgctttc aggtttggca ggtataactt
     7861 atcgatccgc tgacatggtt ggattgctcc agtccctctt tggcggtggc ccgccggaac
     7921 atggtctttc gtgcggaagg acgggcgcat tcaggtgaaa ttagcgccct tcgagccgct
     7981 ccctcgcccg gttggggaag gcaaaggacg aggcccagaa tgtgggcggg tttttgcaca
     8041 tggaatgtga tattctctga gaacagagga aaggttcgag atcgccggcc gtggcaggga
     8101 gctcaagccc tgctcggcca gattcatctt attcagtgcc agttgatgtg gagtgtgttg
     8161 cattgagcag aaccaggatt tatgaattag acgtatttcg cgcaatctgc accataactg
     8221 tgctcttagg gcatgtctca tcgtacccgg ttggcgtttt gagcacggac tccaggtttt
     8281 atggccttta tctaggcgtc aattccttct gtcggtttgc tatccaagcc ttcctctttc
     8341 taagcgctgc ggtgcttgtc cacagctatg ccgatagatg gcaggggccg gagagtgcgc
     8401 ttgcgatgtg gcgaaaaagg cttatcgatc ctggaattcc ttacgtggtc tggtctgtga
     8461 tctatacctt gctggctatt cggcgagggc ggcacataga ctttcctacc tttcttcgcc
     8521 tcttggccac gggaaaggcc tgggaccatc tgtattttat cgttttgata ttccagttct
     8581 acttgctctt ccccctgttg ttgagggctt tgcgatcttt gagctgcagg cccgttttat
     8641 ggctgggcat cggggcgctt attcaggccg ggttccacat gtgggatcaa cgggtatttg
     8701 ccatacccaa cagggcgagt tgggcggcga ggttcggatt ctacttgttc gccgggatgc
     8761 ttgcgggtct cgatttcgat gtcctgcagg attggacgag gcgaaatagg tgggcaattg
     8821 cggcagtgtg ggcggtgagc gccgtgctca actgttcggt cagtgcagcc atacgcctag
     8881 ggactcccca tcggctgtcg ggatatgctg agctgattgt gaacgtgtat gccgttgcca
     8941 gctgcctctt cttcctggcg gtgtcgcaat gggtgacggc gtcaaacggg tggcctatgc
     9001 gcgcggcgat gggtctcagc aatgcgtcgt tcgggattta tctctcgcat ccattgggat
     9061 tggccatgtg gaggcgtctg actgccactg gcaaccccat actcttccac ctgtcggtat
     9121 gggccggggc agtggttgtt ttggcgctgt cgtgggctgt taccctctgg ctcaagcggt
     9181 tcaagttcgg ctggacgctg gtgggaaagt aggggcactg cccctagagg gtttggtata
     9241 tgaccttctt ggccagctca gggtctgcaa taagtctgcc tagccagact tggaaccgag
     9301 tttgtggcct tgccctgtca gagacagagg ccggatgcat atgagtagcc acatcgctcc
     9361 tgcgatgacg ccggcagctg cctctgatgg ccactgaaag cccatgtaga ttccggaaaa
     9421 gcagatcaag gccatgatca cgccggctgc tgccctaatg gcccgccgcc gggctcgtga
     9481 tcgggtgcgg ctgcccagca tgtgggttgc cataccgtag aagggcagcg ctacggggat
     9541 gggctgagcg gcgaagctgc gacaggatat cgggaaccgg aagcgcaagc tctggtcctg
     9601 gaaggctgta cgcc
//
LOCUS       DAOSAI010000042         9508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4723_length_9508_cov_3.239289, whole genome shotgun sequence.
ACCESSION   DAOSAI010000042 DAOSAI010000000
VERSION     DAOSAI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4723_length_9508_cov_3.239289"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLM83_02740"
     CDS             complement(<1..205)
                     /locus_tag="PLM83_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HOK70359.1"
                     /translation="MPIRFILFDLDETLYPASNGLMNLVRDRIIEYIMLRLGVSRREA
                     EEYRLRYFMEYGATLGGLYHEHGV"
     gene            complement(484..1128)
                     /gene="sigH"
                     /locus_tag="PLM83_02745"
     CDS             complement(484..1128)
                     /gene="sigH"
                     /locus_tag="PLM83_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HOK70360.1"
                     /translation="MSVNLQYEIYLHYDELADEEIVELAKQGDSDAEEYLIHKYKNFV
                     RAKARSYFLIGADREDIIQEGMIGLYKAIRDFKPDKLASFRAFAELCITRQIITAIKT
                     ATRQKHIPLNSYVSLNKPIYDEESDRTLLDVLSGNKITDPEELVISREEFNDIEDKMD
                     EFLSDLERDVLNAYLDGKSYQEIAIELNRHVKSVDNALQRVKRKLERYIKKRDG"
     gene            complement(1499..2257)
                     /gene="rlmB"
                     /locus_tag="PLM83_02750"
     CDS             complement(1499..2257)
                     /gene="rlmB"
                     /locus_tag="PLM83_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOK70361.1"
                     /translation="MDDEFIIWGRQPVMEALESDREFNKIFVARGGHDKRIVAAARER
                     RIPVVEVDKAALDRMTASETGVPVVHQGVVAQLSPILYAELDDLAAAAQARREDLFVA
                     VLDGITDPQNLGSIIRTANAVGAHGVVIPARRSSQVSPVVAKASAGAVAYTPVARVGN
                     LSQAIDRLKKMGAWVVGTAADGDRRFFEADLTGPIAVVVGSEGKGMSRLVEESVDFTV
                     SIPMAGEVSSLNAGVAWAVIAFEVARQRLGVNRG"
     gene            complement(2250..2648)
                     /locus_tag="PLM83_02755"
     CDS             complement(2250..2648)
                     /locus_tag="PLM83_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOK70362.1"
                     /translation="MSLLNLAYVGDAVWELFVRSQLAAEGGACIRVNALHRSAEEMVN
                     AEAQSQIVHKLVDFLSPEERDVVRRGRNVKPGHVFRGPDPIDYRYATGFEALLGYMYL
                     AGKDERLREIMEEAMRLAQQKDGVEEPANG"
     gene            complement(2665..4197)
                     /gene="cysS"
                     /locus_tag="PLM83_02760"
     CDS             complement(2665..4197)
                     /gene="cysS"
                     /locus_tag="PLM83_02760"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOK70363.1"
                     /translation="MAIRIYNTLTRRKEEFVPVVPGKVGIYSCGPTVYDYFHIGNARA
                     FVVPDVIRRYLRYRGYNVTLVQNITDIEDKIIKRAAERGVAPQNIVDEFTTAYIEDRE
                     ALGVQAPDIQPRATEHVGDMIDMIKILVEKGLAYVGDGDVYYDVSRFEPYGKLSNQNI
                     DDLVAGARVDVGEAKQDPLDFALWKAAKPGEPSWDSPWGPGRPGWHIECSAMSTKYLG
                     KTFDIHTGGVDLVFPHHENEIAQSHGANGVAPVRYWVHNGYINVDGQKMAKSLGNFKT
                     VRDILQQYPGKVVRYFLISNHYRKPINFSDDELRMCERALSRLEDAIVNAAFTASIDL
                     DNREEMVRLAAAKSGKAWADDSAEARLDGVVKEVRRRFEESMDDDFNTAGAIGSLHDL
                     ATALNTYVNREMPSPPNEEARVAISGAVLALMDLGDVLGIIDPSSMKPAAGNAKEDEG
                     ELANAEKIVEGLVELLLDVRARAREAKEYGVSDMIRDRLAEMDIIVEDTRDGARWKFA
                     SK"
     gene            complement(4250..5539)
                     /gene="ispD"
                     /locus_tag="PLM83_02765"
     CDS             complement(4250..5539)
                     /gene="ispD"
                     /locus_tag="PLM83_02765"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOK70364.1"
                     /translation="MGARLRVTAIVPAAGSGTRMGLAVPKQYLQLRGIPIVAHTVSRL
                     ARSGAVDEIIVSVPPGDEEVFERDIVERYSLSSLCRLKVVAGGSDRQQSVSLALRAME
                     QDLASSATGTEGRGAGVEEAGVDAETCHIVLVHDGVRPFVSKAQIVAVLEAAAVHGAA
                     TCAVAPKDTIKLVGDNGLVAMTPDRSGLCLVQTPQAFHLSILACAHRRAEEEGYVGTD
                     DCSLVERLGLPVALTPGSYDNIKITTPEDLALAEVLLAREDSDCRGESVVSVVRVGQG
                     YDVHRLVHGRDLILGGVRVPYEKGLDGHSDADVLTHAICDAILGAASLGDIGMHFPDT
                     DQEYAGICSVALLERVVALAADAGYRVLGVDATIIAQRPKIAPFVPQMRESLAYAMNV
                     PVEYVNIKGKTTEGLGYIGSGEGIAALAVVSLSPASS"
     gene            complement(5591..6709)
                     /locus_tag="PLM83_02770"
     CDS             complement(5591..6709)
                     /locus_tag="PLM83_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOK70365.1"
                     /translation="MSVRNKMTRVLMLGAGVIFGYLIYRAMPQTGAALSVEGGELRVM
                     VGAMTGGGLVGFAVAPVISRFGLWFGRYVDARIRKVSTHGVISGAVGMIVGLIIANLL
                     AIPIASIPEFGSYIGIALNVFFGVSGLIVGYHKREEIIPMLAPLPRSGENVHVSAPAR
                     DRGIARAYVGKATPKILDTSVIIDGRIADIADSGFIEGTLIVPVFVLEELQHIADSSD
                     VLKRNRGRRGLDILNRMQKGQNVTVQIVDETFDDPEVDSKLIRLGQAMDATILTNDFN
                     LNKVAELHGVEVLNINELANALKPVVLPGEEMTVHVIKDGKESGQGVAYLDDGTMVVV
                     DGGRRYIGDYVDVLVTSVLQTSAGRMIFAKMKSAERVS"
     gene            complement(6796..7272)
                     /locus_tag="PLM83_02775"
     CDS             complement(6796..7272)
                     /locus_tag="PLM83_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOK70366.1"
                     /translation="MYSVGDKVVYPMHGAGVIEAIEEKEVLGEKDYYYIMRLQMGDMK
                     VMVPRGSADEVGLRPVIDCDEVPKVYEVLKAEATPMSQNWNRRYRANMEKMKTGDIFI
                     VAEVVRNLGIRDRDKGLSTGERRMLDNAKQILASELAMVEKRPLEEVANVIDEILS"
     gene            7519..7914
                     /locus_tag="PLM83_02780"
     CDS             7519..7914
                     /locus_tag="PLM83_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOK70367.1"
                     /translation="MADLSCGEFQETVSEYLVRHRSVLDVMSKLQEAGARVNRAVAKS
                     VTSCGCLEVSAQKQKFPENATLADLKRMINSHISGELCPECREALETELGQMLFYIAA
                     LCAITGLDLDEIMMKEHDRLRMLGLFNLT"
     gene            complement(7972..9030)
                     /gene="disA"
                     /locus_tag="PLM83_02785"
     CDS             complement(7972..9030)
                     /gene="disA"
                     /locus_tag="PLM83_02785"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HOK70368.1"
                     /translation="MSERMRKIFQMVAPGTALYDGLENILRARTGGLIVVGDTPEMMK
                     LVNGGFRLDAEFTPAAVYELAKMDGAIILSEDAERIMYANATLTPDPDIPSRETGLRH
                     STAERVSKMTGQLVIAISQRRSVITVYYGHTRYVMRDIGVVLAKANQALQTMANYRDS
                     LVKALTNLTALEFIEEVTLVDVARVVQRYELMRRVGEEVSGYIAELGTEGRLVKIQAD
                     EVMSGMAEEVLFVAMDYMPDGGQVEAEKLMNEIAGWAVDEVLDLATLSRALTQNPSAT
                     PESSMLPRGYRALSKISRLPLSVIGNVVEGFGSLKAIRNATVEELDEIEGIGEVRARA
                     VREGLARLRDQVVRDLVI"
     gene            complement(9030..>9508)
                     /locus_tag="PLM83_02790"
     CDS             complement(9030..>9508)
                     /locus_tag="PLM83_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOK70369.1"
                     /translation="LVEVQALVARSVTGTPRRTTTGVDYNRTAIIIAVLDRRAGFAIG
                     TEDIYVNVAGGVTLTEPAADLGIAAAIVSSFRNIPAAPKTAVFGEGGLAGELRGVSQA
                     DMRVNEAKRLGFKRCVLPSHNVKSMTRRQLAGSGVEIVGASTLREALEAVFAVVDG"
BASE COUNT         2204 a         2959 c         2099 g         2246 t
ORIGIN      
        1 cgaccccgtg ttcgtgatat agccctccca gggtagctcc gtattccata aaatacctca
       61 accggtattc ctcggcctcc cgccgagaga caccgaggcg aagcattatg tactcgatta
      121 ttctgtcgcg cacgaggttc atcagcccat tggacgccgg gtatagtgtc tcgtctagat
      181 cgaacagtat aaatcgaatg ggcacaacca ctcaccccaa gcaaggatat cactgcaggt
      241 taagcctgcg tgggcaggca gttttcgaga ttcacacgat gccattgtca agacgataat
      301 ggcattggct cgcaaaacag agatagagag acgcgccgcg gtcggggaac ctgcatcctc
      361 ccgttccgca gcgcgctgag gaaaatcgat agatgatacg ggcacaaaag ccaatctaag
      421 agcaacatac gcctctccag acttacctcg agagatgggt gcttaagcga gtgcccatcg
      481 cagctatcca tcacgtttct ttatgtaccg ctccagcttg cgtttgactc tttgaagcgc
      541 attgtcaaca gactttacat gacggttcag ttcgatcgct atttcctgat atgatttgcc
      601 atcaaggtat gcgttgagca cgtctctctc caggtcgcta aggaactcat ccatcttgtc
      661 ctcgatatcg ttaaactctt ctctgctgat aacgagttct tccgggtcag taatcttgtt
      721 gcccgacaaa acatccaaca gtgtacgatc tgattcttca tcatatatcg gtttattcag
      781 ggaaacatat gagtttagtg gaatatgttt ctgtctcgtc gctgtcttaa tagcagtaat
      841 aatctgtcta gtaatgcaca gttcggcaaa agcacgaaat gaagctagtt tgtcaggctt
      901 gaagtctcgg atggccttgt agaggcctat cataccctcc tgaattatgt cctccctgtc
      961 tgcgccaatc aaaaaatagg accgggcctt cgccctaaca aagttcttgt acttgtggat
     1021 gagatattcc tcggcgtcgc tgtcgccctg ctttgccaat tctacgattt cctcatcagc
     1081 cagctcatca tagtgcaaat agatttcata ttgcaggttt acgctcaaat gcgtcccctc
     1141 ccgacacaac aagcttagca acgggatatc ttgatacccg agactggctg tgaacaatca
     1201 actaccggct aataaacaag ccggccactt tgacaagagt ttagaaaagc ccacttcggc
     1261 aggtattaag cctgtaccag ctccaaagac tacggccatt atatatcagc gacttcacac
     1321 gagtcaagca aagcgcctgg tagccgctta catatacaat agctatcttt ggcaatcacc
     1381 accttcaacc cacattccaa ccccatttgc catgtacagc cgatggtgcc aactgcggtc
     1441 tgcagcagac gtcatcaagg cagcctctct tgccacaaag cacagcccta tctggctctc
     1501 agcctctgtt gaccccgagt ctctgcctgg caacctcaaa cgctatcact gcccatgcaa
     1561 ctccggcatt aagggaagac acctcacccg ccatcggtat tgagactgtg aaatcaactg
     1621 actcttctac aagtctcgac atgccctttc cctcgctgcc aacaacgact gctatagggc
     1681 ctgtaaggtc tgcctcaaaa aagcgacgat ccccgtccgc agcagtgcca acaacccagg
     1741 ctcccatctt cttaagcctg tcgatggctt gtgagaggtt gcccacacgg gcgacagggg
     1801 tataagcaac tgcgccggca gaggctttcg ccacaacagg gctcacctga gaactccttc
     1861 tagccggtat caccactcca tgtgcgccca ctgcattggc agtgcgaata atggatccga
     1921 gattctgcgg atcggtgatc ccgtccagca ccgccacgaa caagtcctct ctgcgcgctt
     1981 gtgccgctgc cgcgaggtca tcaagctctg catagagtat tggcgaaagc tgcgccacaa
     2041 ctccctgatg aactactgga acacccgtct ccgacgctgt cattcgatcg agcgccgcct
     2101 tgtccacttc caccacagga attctccgct cccgcgccgc cgcaactatt cgcttatcgt
     2161 gtccgcctcg agcgacaaat atcttgttga actctctatc cgactccagc gcttccatta
     2221 caggctggcg tccccatatg atgaactcat catccattgg ctggttcctc gactccatcc
     2281 ttttgttgag ccaatctcat ggcttcctcc atgatttcgc gcagcctttc gtccttacct
     2341 gcgagataca tgtatccaag caaggcttcg aacccggttg cataccgata gtctatagga
     2401 tcagggcccc gaaaaacatg cccgggtttc acatttctgc cccgtctcac cacgtctctc
     2461 tcttctggag agaggaagtc cactagcttg tggacgatct gcgactgcgc ctcggcgttg
     2521 accatctctt ctgccgaacg atgaagagcg ttcacgcgaa tgcacgctcc gccctcagct
     2581 gcaagctgcg aacggacaaa aagctcccaa acggcgtccc caacataagc cagattaagc
     2641 agggacatct cacgcacttg accctcattt agatgcaaac ttccacctcg ccccgtctct
     2701 tgtatcctcg acaattatgt ccatctcagc gagacgatca cgaatcatgt ccgatacgcc
     2761 atactcctta gcctcgcgcg ctctcgccct cacatcaaga agaagctcca ccaaaccttc
     2821 cacgattttc tccgcatttg ccagctctcc ttcatcttcc ttcgcattgc ccgctgcagg
     2881 cttcatggat gagggatcta ttatgccaag aacatcgccc aaatccatca gagccaacac
     2941 cgctcctgag atggccaccc gggcttcttc attgggaggc gatggcatct ccctgttcac
     3001 ataagtattt agagcagttg caagatcgtg tagcgatcct attgcgcctg cggtattgaa
     3061 atcgtcgtcc atcgactcct cgaatcttct tcgaacttct ttcacaactc catccaatcg
     3121 ggcttcagcg ctgtcatccg cccaagcctt gcctgatttt gccgccgcca atcgcaccat
     3181 ttcttcccga ttatccagat caattgacgc tgtaaatgcc gcgttgacaa tcgcatcttc
     3241 caatcgcgaa agtgcccttt cgcacatgcg cagctcatcg tcgctgaaat tgatcggctt
     3301 ccggtagtga ttggatatga ggaaatagcg caccaccttg ccggggtatt gctggagaat
     3361 atcccgaacg gtcttgaagt tgccaagcga tttagccatc ttctggccgt ctacattgat
     3421 atagccattg tgaacccaat accgcaccgg cgccactccg tttgcgccat ggctctgagc
     3481 tatctcgttt tcatgatggg gaaagaccag atcaacaccg cctgtatgta tatcaaatgt
     3541 cttgcccaga tatttcgtag acattgcgct gcattcgatg tgccatcccg gccggccggg
     3601 cccccacgga ctatcccacg agggttctcc cggctttgcc gccttccaga gagcaaagtc
     3661 gagcgggtcc tgtttggcct cgccaacatc gactctcgca cctgccacca aatcatctat
     3721 attctgattc gacagctttc catatggctc aaacctggag acatcgtagt aaacatcgcc
     3781 atctccaaca taggccaaac ccttttcgac aaggattttt atcatgtcaa tcatatcgcc
     3841 aacatgttct gtggccctag gctgaatgtc aggagcctga acaccgagtg cttctctatc
     3901 ctctatgtag gcagtggtaa actcgtcgac aatattctgg ggagccacac ctctttcagc
     3961 agccctcttg atgatcttgt cttctatgtc ggttatgttc tggaccagcg ttacattata
     4021 ccccctgtag cggaggtatc tccgaatgac atcaggcacc acaaaggccc gcgcattacc
     4081 aatgtgaaaa tagtcgtata ctgtaggccc gcagctatag atgcccacct tgcccgggac
     4141 aacaggcacg aactcctctt tacgccgtgt taaggtatta tagattctaa tcgccatggt
     4201 ccagcccccc tccaaagtgc atggcataac ttttgccaga tccgccgagt caactcgacg
     4261 cagggctgag cgacacaaca gccagggccg cgataccttc gcccgaccct atatacccca
     4321 gcccctcagt ggtctttccc tttatattaa catactcaac aggcacattc atcgcatacg
     4381 ccaagctctc acgcatctgc ggcacaaaag gtgctatctt cggacgttga gcgattattg
     4441 tggcatcaac ccccaagacg cgatagcccg catcggccgc caacgcaaca acgcgctcca
     4501 aaagcgcaac gctgcatatt cctgcgtatt cctgatcagt atcgggaaag tgcattccga
     4561 tgtcgccaag gcttgccgcc cccaaaatcg cgtcacatat cgcgtgggtc aacacgtctg
     4621 catctgagtg cccatccagt cccttctcat acggcacccg tacccctcca aggatcagat
     4681 cccgcccatg cacaagtcta tggacatcgt atccctgccc cactctgacg acgctcacaa
     4741 cgctctcacc tcggcaatcc gagtcctccc gcgcaagcag aacctctgcg agcgccagat
     4801 cctccggcgt agtaatcttt atgttgtcgt acgatccggg cgtcagcgca accggaagcc
     4861 ccaacctttc aacgagcgaa caatcatcgg tgccaacata gccttcttcc tctgcacgcc
     4921 tgtgagcgca cgccaagatc gatagatgga atgcctgtgg cgtttgaaca aggcacagcc
     4981 cggatctatc gggagtcatg gcaacaagcc cattatctcc cacgagcttt attgtgtctt
     5041 taggggccac agcacaggtg gcagcgccat gaaccgctgc cgcctccaaa accgcaacga
     5101 tctgagcttt cgacacgaat ggccggactc cgtcgtggac caatactatg tggcaggttt
     5161 cagcgtcgac cccagcctcc tcaacgcccg cacctctgcc ctcagtccca gttgccgaag
     5221 aggcaaggtc ctgctccatc gctctaaggg ccaacgaaac cgattgctgc cgatcggagc
     5281 cacctgctac caccttcaag cggcataacg aagagaggga gtatcgctct acgatatccc
     5341 tctcaaaaac ctcctcatcc ccgggaggaa cagacacgat tatctcgtca acagccccgg
     5401 aacgcgccaa tcgtgatact gtgtgagcca ctatgggaat gcccctaagc tgtaaatatt
     5461 gttttggaac tgccaggccc atccgcgtgc ccgatccagc cgccggcact atggcggtaa
     5521 cgcgcagtcg cgctcccatt ctggctacac ctcgcaagag aatgatcttg ttgagcgaaa
     5581 ccagctaatc tcagctgact cgctcggccg atttcatctt ggcaaatatc atcctgcctg
     5641 ctgatgtttg gagcacactc gtgacaagca cgtcgacgta atcaccgata tagcgcctgc
     5701 ctccgtctac cacgaccatc gtgccgtcgt ccagatacgc tacaccttgg ccagattctt
     5761 ttccgtcctt gattacgtgc accgtcattt cctcaccagg cagtacaacc ggcttgaggg
     5821 cgttcgccag ctcattgatg ttgagcactt ccacaccgtg aagttccgcc accttgttaa
     5881 ggttgaagtc attggtcaga atagtggcgt ccatggcttg ccccaacctg atgagtttag
     5941 aatcgacttc cgggtcatcg aatgtctcgt ctacgatctg gacagtcaca ttctgaccct
     6001 tttgcatccg gttaaggata tcgaggcccc tgcggccgcg atttctcttg agcacatctg
     6061 acgaatccgc gatatgctgc aactcttcaa gcacgaatac gggaacaatc agcgtccctt
     6121 caatgaagcc gctgtccgct atgtcggcta tcctgccatc tattattacg ctggtgtcga
     6181 ggatcttcgg cgttgctttg cctacatatg ccctggcgat gccgcgatcc cgagctggag
     6241 cgctaacgtg gacattttcg ccgctgcgtg gcaaaggcgc aagcatcggg atgatctctt
     6301 cacgcttgtg ataccctaca atcagtcctg acaccccaaa gaagacgttg agggcaatac
     6361 caatatagct tccaaactca ggtatggatg caatcggaat cgcaagcaag ttagctatga
     6421 taaggcccac tatcattccg acagcacccg aaataacccc gtgcgtagac acttttcgga
     6481 tcctggcatc aacatatctg ccgaaccata acccgaatct agaaatcacc ggcgccactg
     6541 caaaaccaac aagcccgccg ccagtcatcg ccccgaccat tactcgcaat tccccgcctt
     6601 ccactgacag ggcagcccca gtctgcggca ttgcgcggta aatgaggtaa ccgaaaataa
     6661 cgcctgcccc aagcatgagc actcgcgtca tcttgttgcg cactgacaca ctcacctcct
     6721 cgcatttgga gggggctgtt ccctccatta tagtctagtc tcccctgggt tcccggggag
     6781 tatgtggagc tcctgttagc taagtatctc gtcgataacg ttcgccactt cttccaaggg
     6841 cctcttttcc accatggcga gctcgctcgc caggatctgc tttgcattat ccagcatacg
     6901 gcgttcgcct gtggaaagcc ccttgtccct gtcgcgtatt ccaagattgc ggacaacctc
     6961 ggcaacgatg aagatatccc cagtcttcat cttctccatg ttcgccctgt atcttcgatt
     7021 ccaattctgg ctcatcggcg tcgcttcagc cttaagcacc tcataaacct tgggaacctc
     7081 gtcacaatct ataaccggcc tgaggcctac ttcatctgcg ctgccgcgag gcaccatcac
     7141 cttcatgtcg cccatctgca agcgcatgat gtagtaatag tccttctccc cgaggacttc
     7201 cttctcctca atagcctcga tcaccccagc accatgcata gggtagacca ccttgtcacc
     7261 aacactgtac acctcttgca tcccttcttc ctcggagcgc cttctctgaa ttgtaccatt
     7321 gcctggcaaa gcagtcaatt ccgccgcata gatccctcaa ccaccagcca tccccgtgaa
     7381 aaaggcacag ccctttgcag acaaataatc cagccacacg gagaagcgga ttgacaatac
     7441 aattgcccaa acgtataatc aagatgaatt ttcagcggcc atatcacctg tgttttccgc
     7501 ttttggaggt gaatcgcctt ggccgaccta tcatgcggcg agtttcaaga gacggtctcc
     7561 gaatatcttg tccgtcatag aagcgtcctc gacgtaatgt caaaacttca ggaagccggg
     7621 gccagagtca accgtgcagt cgcaaaatcg gtgacttcct gtggttgtct ggaagtttcc
     7681 gcacagaagc agaaatttcc ggagaatgcg accctggcgg acctgaaacg catgatcaac
     7741 tcgcacatca gcggcgagct ctgcccagaa tgcagggaag cccttgaaac cgaattagga
     7801 caaatgttat tctacattgc agccctatgc gccataacag gacttgatct tgacgaaata
     7861 atgatgaaag aacacgaccg cttgcgcatg ctgggcctgt tcaatctgac gtaattgagg
     7921 gggagcggta tcgctgcccc gtcgcctatt cgcagggcag attttccgca tctagataac
     7981 caaatcacgc actacctgat cgcggagacg ggctagtcct tcccgtactg ccctcgctcg
     8041 cacttcgccg atcccttcga tctcatcaag ttcctcgacg gtggcgttcc ttatggcttt
     8101 cagcgatcca aacccttcaa ctacgtttcc gatgacagac agtggcaacc gagatatctt
     8161 cgacaacgcc cggtatcccc ttggcaacat cgatgactca ggagttgcag acggattttg
     8221 agtcagggcc ctcgacaagg tggccagatc caaaacttcg tctactgccc aaccggcaat
     8281 ctcattcatc agcttttcgg cctcaacctg ccctccatcg ggcatgtagt ccatagctac
     8341 gaaaagaacc tcctcagcca ttccgctcat gacctcgtca gcctggattt tcaccaatct
     8401 gccctcagtg ccaagttcgg caatatagcc agatacctct tcgccaaccc gcctcataag
     8461 ctcgtatctc tgaactactc tggcaacgtc aaccaatgtg acttcctcta tgaattcgag
     8521 agcagtcagg ttcgtgagcg ccttgaccaa tgaatcacgg tagttggcca tagtctgcaa
     8581 tgcctgattg gccttggcca gcacaacacc tatatcccgc ataacatatc tggtgtgtcc
     8641 gtagtagacc gtaatcaccg accgcctttg ggaaatggcg atgactaact gcccagtcat
     8701 tttcgatacc cgctccgccg tgctgtggcg aaggcccgtt tccctcgatg gtatgtccgg
     8761 atccggagtg agggtggcat tggcgtacat gatccgttcg gcgtcttccg aaaggattat
     8821 agccccatcc atcttggcca attcatacac ggccgccggg gtaaactccg catcaagcct
     8881 aaagccccca ttaactagct tcatcatctc aggcgtatcg cctacaacaa ttagcccccc
     8941 agtcctcgca cgaaggatgt tctccagtcc gtcatataga gccgttccgg gcgccaccat
     9001 ctgaaatatc ttgcgcattc tctccgacat cagccatcca ccaccgcaaa taccgcctcc
     9061 aatgcctctc taagagtgga agcccctact atctccaccc cggatcctgc caattgccgt
     9121 ctggtcatgc tcttcacatt atggctcgga agaacgcatc gtttgaagcc gagcctcttg
     9181 gcctcattga ctctcatgtc agcctggcta acgccgcgca actctccggc caatccgcct
     9241 tcgccgaaga ccgctgtctt tggagctgca gggatgttcc taaagctcga cacgatggcg
     9301 gccgcaattc caagatctgc ggcaggctct gtcagcgtga ctcctcctgc cacatttacg
     9361 tagatatcct ccgtgcctat ggcgaatccc gcacgcctgt caagcaccgc gatgatgatc
     9421 gcagtcctgt tgtaatcgac acccgtagta gtcctgcgcg gcgttcctgt aacagaacgc
     9481 gccaccaatg cttggacttc gacgagca
//
LOCUS       DAOSAI010000043         9457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4761_length_9457_cov_3.931078, whole genome shotgun sequence.
ACCESSION   DAOSAI010000043 DAOSAI010000000
VERSION     DAOSAI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4761_length_9457_cov_3.931078"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /gene="rsgA"
                     /locus_tag="PLM83_02795"
     CDS             <1..727
                     /gene="rsgA"
                     /locus_tag="PLM83_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOK70370.1"
                     /translation="VIEEVMPRKTQFSRKVAGKRRDEQVIAANLDQVVIVFAAANPPM
                     KETAINRYLTTAEYCGLRSVVCINKADLVSIEVLGEKMSVYSDIGYDVIIASTVSGVG
                     ISTLRETLSGKMSALVGPSGVGKSSLISAVEPGLDLAVGDVSGSTGKGRHTTTSGRLI
                     KLAGGGYVADTPGMREFGFYRIPRQELAWCFREFRPHIDACKFWDCTHTTEPGCRLRE
                     AVEQGLVSQARYEHYVRLMEEMR"
     gene            complement(787..1419)
                     /locus_tag="PLM83_02800"
     CDS             complement(787..1419)
                     /locus_tag="PLM83_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70371.1"
                     /translation="MAQDISIAALLAEEGFNTLAASRSARETLVRNGLTNHRKKNISV
                     QKLSRVREVLARELYRVCDDPWCQAIAGMEARGREIVTVAEDGCQVCRGAKSCRSLKA
                     AALVFERRQIRRVVIVGGTKAGHNEIAAHACGSGVEFRFVDGTKPGRASQLAAEYARW
                     ADLMIVWANTPIKHTVSYTYKGACPKSTRLVYATRTGVAGMLYEVISQIA"
     gene            complement(1553..1786)
                     /locus_tag="PLM83_02805"
     CDS             complement(1553..1786)
                     /locus_tag="PLM83_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70372.1"
                     /translation="MRLKVLTVLVIEDDHNVRRLLSRYFEAVASRCVNGQLEMDIKWV
                     EEDLLIVETLPRQQAQVDWGQCGKSGRQHHGLA"
     gene            complement(1923..2585)
                     /locus_tag="PLM83_02810"
     CDS             complement(1923..2585)
                     /locus_tag="PLM83_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70373.1"
                     /translation="MNGGTHGFPTMQQYFAKRYRVWAIISFVIVLVLSLVAVDSIISK
                     SAARTMLRDLEAGIRKGERGLLGGLLAKDRGGTLNIAPDAVGDYVDTWPDVQRVQDRG
                     KGSGHGNVPWTSGSVVWAARKWHDGTIEVTWTRLLGVRKDATGIYLLVTAAVAVSFWI
                     SAVIAKQGAKRISQMVGSAAEASLKISEGDMELDMEFRATQELSDISDAVNRLASKLR
                     RE"
     gene            complement(2782..3096)
                     /locus_tag="PLM83_02815"
     CDS             complement(2782..3096)
                     /locus_tag="PLM83_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006824501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOK70374.1"
                     /translation="MRKTYDKLVRDRIPEIIEASGKQAVTRIADDSEYEMYLKQKLME
                     EVNEYLASGNPEELADIMEVVAALGQASGIKFEDLVMMAEKKRHSRGGFDSKIVLLHV
                     DD"
     gene            complement(3099..3485)
                     /locus_tag="PLM83_02820"
     CDS             complement(3099..3485)
                     /locus_tag="PLM83_02820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOK70375.1"
                     /translation="MPQCIFCNLPKEKVIAENDLALAFYDAYPVNEGHVLIIPKRHVE
                     TYFDATQDEKNAISLLLAKAKEKLDERFHPDGYNIGVNVGAAAGQTIFHLHVHLIPRY
                     AGDVPDPRGGVRKIKKSLVPYAEEGE"
     gene            complement(3506..5830)
                     /locus_tag="PLM83_02825"
     CDS             complement(3506..5830)
                     /locus_tag="PLM83_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOK70376.1"
                     /translation="MPRLEAAIGRADRIRLIVAFVMESGVKLILPSLQQAASRGVSIE
                     LLTGQYLSITQPSALYLLYDRLGGSIDMRFYNGSIEAFHPKTYIFDHGDEAEVFVGSS
                     NLSRSALTTGIEWNYRFFRSEHPSEYHKFSDTFDELFHKYAQPATAEVLKEYALAWKK
                     PALIRDVETSTQTVWTGKPEPRDAQIEALYYLKQARDEGASKGVVVAATGVGKTHLAA
                     FDSLEYERVLFLAHRQQILDQAYEVFRSVRPESHFGLYAGEQKDEGACIYFSTVQTLS
                     RDEHLDVFPKDYFDYIVVDEFHHAAADTYRKVMEHFEPRFLLGLTATPFRMDNKDIFS
                     LCDDNVVYEISLKQSIERDLLVPFHYYAIYDPTNYDDVRIANGSYVIEDLEKQLSHVE
                     RADLVLEHYRKLAGQQCLGFCVSIGHAEYMAEYFSAHGIPSAAVHSGELGNGYALERD
                     NAIEALEEGRIKVIFAVDMFNEGVDVPSLDTVMFLRPTESYVIFLQQLGRGLRKYPGK
                     HHLTVIDFIGNYKRAHHIPLLLAGDNPWTHEFSRCLRIEDYEFPEGCTVNFDLRVIDL
                     FDELAARDPLPVRMQDTYWKIKHSLGKRPNRVDIYEGSDIPMREYLRDGWLRFLESVG
                     QLEPEEASWLDTPAEAFLVEIEQTRMTKAYKLPTIAAFLRDGDIVAQARLDEIGERMM
                     RFYVDHPVHQKDFRDKSNRKWRQWTADDFAELARKNPVRFLSRGQFFHYDEINRVMYI
                     DSQVQPYLSPHLAEHVQDILSYRRIDYFRRRFKD"
     gene            complement(5928..6788)
                     /locus_tag="PLM83_02830"
     CDS             complement(5928..6788)
                     /locus_tag="PLM83_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOK70377.1"
                     /translation="MHVQTVEYKRVDGCSICADVYGSSIPSSPAVVYIHGGGLIWGSR
                     KRVVPEQISAFVGQGYTVISIDHRLAPQTKLPSIVEDVCDALSWVLDDCPRFFGIDPD
                     RVAVFGRSAGAYLALLSGTLARKPKAVVSYFGFGDLLTDWGNKPSGYHLSMPEVSKKR
                     AYECIGSNVISGSGEERLPFYIYCRQQGIWMREVVGLNRDDRVLKEYSPMHNVRSDYP
                     PTLLMHGEEDKDVDCTESVKMANALTHAGVKNELITLPGLGHSFDRQMGDARVRESLE
                     SMLAFLRDNL"
     gene            7209..8510
                     /locus_tag="PLM83_02835"
     CDS             7209..8510
                     /locus_tag="PLM83_02835"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOK70378.1"
                     /translation="MPYVGLAVGTGLLLIAVRHKVNVGLAATIAALVVTAMSGNGIAM
                     LAGALHDTFIDPNGLTLLVSIALITMLGSVMRTSGATDALAQGIRSLVKDDRWVVFLI
                     PSLMGFLSVPGAAVFSAPVIDSVGDALDMSREQKVTANIMFRHAWYYIYPLSTTLLLT
                     QQVSGVPYSAFALPGSIACIIVILSTAKLVFPQEAPPMEPSGTHSRTAFGLDAQSRLP
                     SAVISRARTTFPMILISIAPIIVVLLFALATPVSFPLAVAFSVATGVFVVQPGKPYWQ
                     EVNRRFREGVVRGIDWPVVATVAGTVLLGKTVANSPALDIASASMQTSPASLLLFGIA
                     MPIAAAFVLGSHTAAVAITAPLLLPLIADLPTPFGFVVALFMSSFSGYLVSPAHVCLT
                     ATTQYLGADLGESWRLLIIPSGLSLLFTLAYWGWNALFNLM"
     gene            complement(8619..>9457)
                     /locus_tag="PLM83_02840"
     CDS             complement(8619..>9457)
                     /locus_tag="PLM83_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70379.1"
                     /translation="DFAFWRDGEGRIVGCHDKGTVMIDYDDERVAYSYEGSDLFGYVD
                     LGYSGEALTDSEWLDLTARCQWPAVPVQIVGYMQGDYSGDVVVVTNPPKGMPNASGFV
                     ARHKGVAASDCTVPVLLVGPDINLDPVPDSIWLQTLYSDVLGIDTVNTLRAEREPHSL
                     SLSSAGVGCTLSPAPSLVLQLELGKGSSDFLCEIDVFRTFNTRIWIGAGIVADRASSL
                     SPCGTFSTRSELFSGRMVFELRKLRFDHKVEQQTSVRYDVGNGITLRWTYPDQFSLGV
                     TW"
BASE COUNT         2235 a         2726 c         2256 g         2240 t
ORIGIN      
        1 cgttatcgag gaagtgatgc cgcgcaaaac tcagttttcg cggaaagtgg caggcaagcg
       61 ccgagatgaa caggttatcg ccgcgaactt ggatcaggtg gtcatagtgt tcgccgcggc
      121 caacccgccc atgaaagaga ccgccataaa cagatatcta actacagcgg aatactgcgg
      181 cctcagatcc gtcgtatgca tcaataaggc ggacctggta tccatagagg ttctcgggga
      241 aaagatgtcg gtctacagcg atatcgggta tgacgtcatc atagcatcta ccgtgtcggg
      301 tgtcggcatc agcacgctgc gcgagactct ttccggcaag atgtcggctc ttgtcgggcc
      361 gtccggggta ggaaagtcgt cgctcatcag cgccgtcgag ccgggcctgg atcttgcagt
      421 aggtgatgta agcggaagca ccggaaaagg cagacatacg acaacttctg gacggctgat
      481 caagctggca ggcggcggat acgtggcgga caccccaggg atgagggaat tcgggttcta
      541 ccgtatccca cgccaagaac tagcatggtg tttcagggag ttccggccgc acatagatgc
      601 gtgcaagttc tgggactgca cccacacaac agaaccaggg tgccgcctcc gagaagccgt
      661 agaacaaggc ctagtttcac aggcacgata tgaacattat gtgagactta tggaagagat
      721 gcggtagggc attcgccagc cctctgcgca ggttgtggag agtacgaccc cgcagagggc
      781 tgtttttcaa gctatctggc tgatgacttc atatagcatt cctgccaccc cggtccgcgt
      841 ggcatataca agccgggtgg actttggaca cgcaccttta tatgtgtagc tgacagtgtg
      901 tttgatcggg gtgttggccc agactatcat caaatcggcc cagcgggcgt attctgcggc
      961 gagctggctg gcccggccgg gtttggttcc gtcgacgaac cggaattcta caccgctacc
     1021 acaagcgtgg gcagctatct cgttgtggcc ggccttcgtg ccgcccacga taacgacccg
     1081 gcggatctgc cgcctttcaa agaccagcgc tgctgccttt agcgacctgc atgattttgc
     1141 gcccctgcac acttggcacc cgtcttctgc aacagtgacg atttcccgcc cacgagcctc
     1201 catgcctgca atcgcctggc accatggatc gtcgcacaca cgatacagtt cccttgcaag
     1261 aacctcacgg acccgggaca gcttctgcac gcttatgttc ttcttccggt ggtttgtgag
     1321 gccgttgcgc acgagcgtct cccgagcaga tcgactggca gctaaagtgt taaacccctc
     1381 ctccgccaac aaagcagcga tggaaatatc ctgtgccatc tgcgtcagcc tccctccgta
     1441 gttccggtaa gacaaggata acagatagcc ccggcacaga cttgtcagtt acgtccgata
     1501 taatgctgta aaaaggccac tggttctcta gaggtaacga attgaatgcg cctcacgcga
     1561 gaccatgatg ttgacgcccg ctctttccac attggcccca atctacctga gcctgttgtc
     1621 taggaagggt ctctactatc aataagtctt cttccaccca ctttatgtcc atttcaagtt
     1681 ggccattaac acatctactc gcaaccgcct caaaataccg ggaaagcagg cgtcggacgt
     1741 tgtggtcgtc ttctattaca aggacagtca gtaccttcaa gcgcaatacg gtgtgccttc
     1801 ttcaggtacc cggttgaggt ggacaacgag tcgttttgaa acaaggcagc acattctgcg
     1861 ctggcgacac cagcatttaa tccatgcatt cgagtaaggt gaggccgcgt ctcaagctgc
     1921 atctactctc ttcgaagctt tgaagcaagc ctgttgacgg cgtctgatat gtctgaaagc
     1981 tcctgggtcg cccggaactc catatcgagc tccatatcgc cctctgatat cttcaagctc
     2041 gcctcggcag ctgaccccac catctgggat attctctttg caccctgctt ggctatgacg
     2101 gcactgatcc agaaagaaac cgccactgca gcggtaacaa gtagatagat gccagttgca
     2161 tccttcctaa cgccgagcag gcgggtccac gtgacctcaa ttgtgccgtc atgccatttt
     2221 cgggccgccc agaccactga cccgctggtc caagggacat ttccgtggcc ggatcccttt
     2281 ccccgatcct gcacccgctg aacatccggc catgtgtcaa catagtctcc aactgcatca
     2341 ggggctatat tcaaggttcc accccggtcc ttggcaagca agccgcccaa caggcccctc
     2401 tctcctttcc ggatgccggc ctccagatcc cgaagcatag tgcgggctgc tgatttcgat
     2461 atgatagaat caacagcaac aagcgagagt actagaacaa tgacgaaact tataattgcc
     2521 caaacgcgat accttttcgc aaaatactgt tgcatggtag gaaacccgtg tgttcctccg
     2581 ttcatgaaaa tccactcctt tgcatgatcc cacattctgg cctagccaag cccgtgtcgc
     2641 agaagtaaga ctgtcaatca gttcttcttt ggataatcca tccataccgc atctgtactg
     2701 ataaatagca caaaggccaa cagatatcct ccattggccg gcgacaaata tacaattgtt
     2761 gcagtttaaa tgcacaacgg gctaatcgtc gacgtgaagc agcacaatct tggaatcaaa
     2821 gccacctcgg gagtgtctct tcttctcagc catcattact agatcctcga acttaattcc
     2881 agacgcctgc cccagagccg cgacaacttc cattatatct gcaagttctt caggatttcc
     2941 actggccaga tactcattga cttcctccat cagtttctgc ttgagataca tctcatactc
     3001 actatcatcg gcgattcgag taaccgcctg tttccctgac gcttcgatga tctctgggat
     3061 tctatctcta accagcttat cgtaggtttt gcgcaaagct actcgccctc ctcagcgtat
     3121 ggaaccagac tcttctttat ctttctgacg ccgccccgcg gatccggcac atcccctgcg
     3181 tatctgggaa tgagatgaac gtggagatgg aagattgtct gtccagcagc agcacccaca
     3241 tttaccccta tattgtagcc atccggatga aaacgttcat ccagtttttc tttggccttt
     3301 gcaagcagaa ggcttattgc gttcttctca tcttgagtgg catcgaaata ggtctcaacg
     3361 tgccgtttgg gaatgatgag aacatgcccc tcattgaccg gataggcatc gtagaatgca
     3421 agagccagat cgttttcggc aatcaccttt tccttcggca aattgcagaa tatgcactga
     3481 ggcaagaacc ccacctcttt ctagttcagt ccttaaaccg gcgtcggaag tagtcgattc
     3541 gtcggtatga gagaatatcc tgcacgtgtt ctgcaaggtg cggcgaaaga tacggctgca
     3601 cctgagaatc gatatacatc acacgattta tttcatcata gtggaaaaac tggcctcgcg
     3661 acaagaatct gaccgggttt ttcctggcca gttcggcgaa atcgtcagct gtccattgac
     3721 gccacttgcg attgctctta tccctgaaat ccttctgatg cacgggatgg tcgacgtaga
     3781 aacgcatcat tctttcaccg atctcgtcca gtcttgcctg cgccacaata tcgccgtcac
     3841 gcaaaaatgc cgctattgtc ggcagtttgt atgccttagt catccgggtc tgttcaatct
     3901 cgacgaggaa tgcctctgcc ggcgtatcga gccaactggc ctcctcaggt tcaagctggc
     3961 caacggattc gaggaacctg agccatccat ccctgagata ctctctcatc ggtatgtcac
     4021 tgccctcata gatgtccact cggttcggcc ttttacccag ggagtgtttg attttccaat
     4081 aagtatcctg cattctcact ggcaaggggt cgcgcgccgc caattcatcg aacaagtcga
     4141 tgactcgtaa gtcaaagttt acagtgcacc cttccgggaa ttcgtaatct tctattcgca
     4201 gacaccttga aaattcatgg gtccacgggt tatccccggc aaggagaagg ggaatatggt
     4261 gagccctctt gtagttgccg ataaagtcga tcaccgtaag atgatgtttg cctggatatt
     4321 ttcgcaatcc cctgcctagc tgctgaagga atatcacata tgactcggtg ggccgcagaa
     4381 acatcacagt atcgagggaa ggaacatcaa caccttcatt gaacatgtcg acggcaaata
     4441 tcactttgat tcggccttcc tctagtgcct ctatcgcgtt atctctttca agcgcgtatc
     4501 cattgcctag ctctccacta tgaactgcag ccgaaggaat cccgtgagca gagaagtatt
     4561 ccgccatgta ttccgcatgc ccgatggaaa cgcaaaagcc caaacactgc tggcccgcga
     4621 gtttgcgata gtgctccaaa accaaatctg ctcgctctac atgcgatagc tgcttctcaa
     4681 gatcttcaat aacataagaa ccattggcta ttctcacatc atcatagttt gtagggtcat
     4741 agatcgcata gtaatggaac ggcaccaaca agtccctttc gatggattgc ttgagtgaga
     4801 tctcatagac gacattgtca tcacataaag agaagatgtc cttattgtcc atcctaaatg
     4861 gcgtggcagt caggccgagc aggaaacgcg gctcgaaatg ctccattacc ttgcggtagg
     4921 tatcggctgc ggcatggtga aactcgtcca ctacgatgta gtcgaagtag tccttgggga
     4981 acacatccaa atgctcatca cggctgaggg tctgcacagt ggaaaaatag atgcatgcgc
     5041 cctcatcttt ttgctcgcct gcgtaaagcc cgaagtggga ttcgggccgg acggacctga
     5101 atacttcgta ggcctgatcg agtatttgct gccgatgcgc gaggaaaagc actcgctcgt
     5161 attcgagcga atcaaaagct gccaggtgag tcttgccgac gccggtggct gcaacaacca
     5221 ctcctttgga tgcgccttca tcgcgtgcct gcttcaggta gtatagagct tctatctgag
     5281 catcccgggg ctcaggcttc ccagtccaga ccgtctgggt actcgtctcg acatcccgaa
     5341 tcagcgctgg tttcttccag gctaaagcgt attcctttag cacttccgca gtggccggct
     5401 gcgcgtattt gtggaataac tcgtcaaagg tgtcgctgaa tttgtggtat tcgctcgggt
     5461 gttcgctacg gaagaaacgg taattccact cgatcccagt ggtcagggcg gagcgtgaga
     5521 ggttggatga tcccacgaaa acctctgcct cgtccccgtg gtcaaaaatg taggtcttgg
     5581 gatggaaagc ttcaatgctc ccgttgtaaa atctcatgtc tatggagccg cctaatctgt
     5641 cataaagaag gtacagggct gaaggttgcg ttatggacag atactgccct gtcagaagct
     5701 ctatagacac gcctcggcta gccgcctgct gaagacttgg caggatgagc ttcaccccag
     5761 attccatgac aaatgccaca atcagccgta tcctgtcggc tcttccgatt gccgcctcca
     5821 aacgtggcaa taggtgatca tccagaccag ttatggcact gatcacaaaa cccacctcac
     5881 aacagactca agcacactcc atgagtcgaa tagctgcatc cacctgtcta tagattatcg
     5941 cgcaagaaag ccagcatgga ctcgagtgac tccctaaccc gcgcgtcccc catctggcgg
     6001 tcgaaggagt gccctaggcc gggaagggtt atcagctcgt tttttactcc tgcgtgagtc
     6061 agtgcattgg ccatttttac ggactccgtg caatccacat ctttgtcttc ctcgccatgc
     6121 atcaacagtg taggggggta atccgacctt acattgtgca tgggcgagta ttccttcagg
     6181 actctgtcat ctcggttgag gccaaccact tcgcgcatcc agataccctg ttggcggcag
     6241 tagatataga acggcaaacg ttcttcaccg cttccggata taacattgct tcctatgcat
     6301 tcatatgccc gtttcttcga aacttcgggc attgacagat gatacccact tggcttattc
     6361 ccccaatcag tgagaagatc gccaaatcca aagtacgaaa cgacagcttt cggctttcgg
     6421 gcaagggtcc cgctgagcaa cgcgagatac gcgcctgccg acctgccgaa tacagcgact
     6481 cggtcagggt cgatgccaaa gaacctgggg cagtcatcca atacccagct cagagcgtcg
     6541 cacacatctt caacaatgga cggcagcttt gtttgggggg caagacggtg gtctatggat
     6601 atcacagtat acccctgacc cacaaaggca gagatttgct ccggcacaac ccgcttccgc
     6661 gatccccata tcaaaccccc gccatgtatg tacactacag cgggagagct tggaatggat
     6721 gaaccataga catcagcaca tatgctgcat ccgtcaacgc gtttgtattc cacggtctgg
     6781 acatgcattc caaacgaaac ctccctcatc ttgagccatc agtctacatt ctcgcctacc
     6841 cgtcccactc cgcaaccatg aacttcacat acaccgctcc cggccgaagc aaggcttatg
     6901 taaaatctgt aacaaccaca caaggctgca caatactgag catcctagtg gttgcgctac
     6961 aacctcaatc cgcccacttg cgcaaagcgt aagatggggc cgctagcctg cgggagaatc
     7021 tgaacagtct ttgctacata gcccactgca gctccacagt ccctaggcat acatactcca
     7081 cggtgttctc aattccttta ctaacgcccg aatatcgaaa ctgccttcct gacacgttca
     7141 gcattgaacc agtgccggat gtcgaaatat actgggatgt ggtagcatgt tctcggaggt
     7201 gccagttgat gccttatgtg gggctcgcag taggaactgg cctattgctc atcgccgtca
     7261 ggcacaaagt aaatgtggga cttgcagcca caatagccgc gctggtcgtt acagctatga
     7321 gcggcaacgg aatcgccatg cttgctggag cattgcacga tacatttatt gatccgaacg
     7381 gcctcacctt gcttgtttcc atcgccctca tcaccatgtt agggagtgta atgagaacct
     7441 cgggagcaac cgacgccttg gcacagggaa taagaagcct agtcaaagat gatcgctggg
     7501 ttgtcttcct catcccttca cttatgggct tcctcagcgt tccaggggct gcagtcttct
     7561 ccgcgccagt catcgattct gttggagatg ccttggacat gagccgagaa cagaaagtga
     7621 ctgccaacat catgttcaga catgcatggt attacatcta tcccctctcc acaacccttc
     7681 tcctcaccca acaagtctcg ggcgtaccat atagcgcctt cgcattgcca ggctccattg
     7741 cctgcataat agtaattcta tccacagcca agctggtgtt cccgcaggaa gcaccgccaa
     7801 tggagccttc gggtacacac tcaagaaccg cctttggctt agacgctcaa tccaggctgc
     7861 cgagcgcagt aatatcccgg gcccgtacaa cattcccgat gatcctgata tctatagctc
     7921 cgatcatcgt agttctgctt ttcgccctgg caaccccagt cagtttcccg ctggctgtag
     7981 catttagcgt ggcaacagga gtctttgtgg tgcaaccggg caaaccctac tggcaagagg
     8041 taaacagaag atttagggaa ggcgtagtgc gcggcatcga ttggcccgta gttgcgacag
     8101 tggcaggcac cgttcttttg ggcaaaacag tggccaactc gccagcccta gatatagcaa
     8161 gtgccagcat gcagacaagc ccggcatccc tgctcttgtt cgggatcgcc atgcccattg
     8221 cagctgcctt cgtgctaggc tcacataccg ccgccgtagc catcacagcc ccccttctcc
     8281 tgcccctaat cgcagacctg ccgactccct tcggcttcgt cgtcgccctc ttcatgtcat
     8341 cgttttcagg gtatctcgtc tcccccgcac atgtctgttt aaccgctaca acacaatatc
     8401 tcggggctga cttgggggaa agctggcgac tattaataat accgagcggt ctcagccttc
     8461 ttttcaccct tgcttactgg gggtggaatg cgcttttcaa cctcatgtag gctcctctgt
     8521 tccttaccac tccactgccc cacatgttgc gcaccccttc gctctagaac actctcacat
     8581 gctcaacacg gccatgggtt tctctcgcaa aaccatatct accaagtcac tcccaggctg
     8641 aactgatccg gataagtcca cctcaatgtg ataccattac caacgtcata cctgacagat
     8701 gtctgctgct caaccttatg gtcgaatctg agtttgcgca attcaaaaac catcctgcca
     8761 gaaaaaagtt ccgacctggt cgaaaaggtg ccgcatggtg ataatgaaga tgccctatca
     8821 gcaactatcc ccgccccaat ccagatcctc gtgttgaaag tacggaacac atcgatctca
     8881 cacagaaaat cgctagatcc cttcccgagc tctaactgca gcaccaagct cggagcaggc
     8941 gatagggtac acccaacccc ggctgacgaa agagacaagc tgtgtggttc cctttcggca
     9001 cgcagagtgt tgactgtgtc aatgcccaac acatcgctgt acagagtctg cagccagatg
     9061 ctatcaggca caggatcgag gttgatgtca gggcccacaa gtagcaccgg cactgtgcag
     9121 tcagacgctg caacaccttt gtgacgggca acaaatccgc ttgcgttagg catccctttc
     9181 ggaggattag tcactacaac cacgtctcca ctgtagtcac cctgcatgta tccgacaatc
     9241 tggactggta cagccggcca ttggcaccgc gcggtcagat ccaaccattc cgagtcggtt
     9301 agagcttcac ccgaataccc gaggtcaaca tagccgaaca gatcgcttcc ctcataggag
     9361 taggcaaccc gctcgtcatc ataatcgatc atcactgtcc ctttgtcatg gcagccgact
     9421 atccggccct ctccgtcacg ccaaaaggca aaatcaa
//
LOCUS       DAOSAI010000044         9434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4772_length_9434_cov_3.080179, whole genome shotgun sequence.
ACCESSION   DAOSAI010000044 DAOSAI010000000
VERSION     DAOSAI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4772_length_9434_cov_3.080179"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..439
                     /locus_tag="PLM83_02845"
     CDS             305..439
                     /locus_tag="PLM83_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70380.1"
                     /translation="MTVGDVIKAAPDPGPFGCGFGSWGMLLILLAIIVLAAFLPRFCW
                     "
     gene            764..1126
                     /locus_tag="PLM83_02850"
     CDS             764..1126
                     /locus_tag="PLM83_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOK70381.1"
                     /translation="MKSLDDLKKIKERAQAMTELREGNEEVRIVIGMGTCGIAAGARD
                     TLAAVVDEINTRNLRHVAVTQTGCIGMCEYEPIVDVIRQGEPKVTYGRVTPEKARQII
                     ASHVVNGQVVGDLVISSK"
     gene            1255..>1489
                     /locus_tag="PLM83_02855"
     CDS             1255..>1489
                     /locus_tag="PLM83_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOK70382.1"
                     /translation="MLKMRGPVGPEGPGVVCECDDVFLTEVQRVVSVYKERPEDLVQA
                     LHDVQSLRNYLPREALRVVARTLDVPESRVYGVA"
     assembly_gap    1490..1499
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1500..1769
                     /locus_tag="PLM83_02860"
     CDS             <1500..1769
                     /locus_tag="PLM83_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOK70383.1"
                     /translation="MFSTSPRGRHIIRICESAPCHVAGAAEIINSLLNELGVEMGGTT
                     SDGIFTVETSSCLGVCGVAPAIMIDDTVYGNLVSEDLPAVLRRYR"
     gene            1807..3480
                     /gene="nuoF"
                     /locus_tag="PLM83_02865"
     CDS             1807..3480
                     /gene="nuoF"
                     /locus_tag="PLM83_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOK70384.1"
                     /translation="MEFYRAHVLVCGGFPCVAAGCRAVRGEIERSVKEHGLEREVRIV
                     ETGCLGPCDLGPIVVVYPEGTVYSRVSVDDVSEIVGEHLMKGRPVRRLEYTERLPSVS
                     QADHKRPDYFGIQERIVLENVGLIDPDSIEEYIARDGYLGAGMALTSMSPDEVIGAIK
                     ASGLRGRGGAAFPTGVKLGFTARVEADQKYIICNADEGEPGTFKDRLILEGDPHKVIE
                     GMIILGYSVGATMGYIYIRGEYYLSIERIERAIAEARRYGLLGHDLFGSGFSFDIEVK
                     KGAGAYVCGEETALIESMEGKRGEPRQKPPYPGTCGLWGKPTVVNNVETIANVPPIVR
                     NGADWFRQFGTPTSPGTKVYTLTGDVNNKGLIEVPMGITLREVIYGVGGGIPGGRRFK
                     MAQTGGTAGGCLSETHLDIPMDYESLQEAGSALGSGALLIIDDSHCVVDIACALLRFF
                     EHESCGQCTPCREGTRRLHDIFRKTRELAATEKDLKLARELAGVMQASSLCALGQSVA
                     VPLFTIMDHYADEIEAHLEGRCPAGVCRPNPANADESAYETLQAGNGIR"
     gene            3500..5216
                     /locus_tag="PLM83_02870"
                     /pseudo
     CDS             3500..5216
                     /locus_tag="PLM83_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
     gene            5420..6760
                     /gene="rsxC"
                     /locus_tag="PLM83_02875"
     CDS             5420..6760
                     /gene="rsxC"
                     /locus_tag="PLM83_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOK70385.1"
                     /translation="MPKGSFRGGVHPPENKERTATLRTVVARRPSQVAIALHQGSNCP
                     LTPTVAKGDYVRMGQKVADTDEKWGVPAHSPVSGKVVSIGLVRTASGVEDQAIFIEPD
                     GEDALDESCVPVDNVFSRSPEEIIRIIRDAGIVGMGGAEFPTHIKLSLPPGANVDTLL
                     LNGAECEPYLTADHRLMVERPRDIIRGAQIMMRAIGVSRTLIGIEDNKPDAIRAMGDA
                     ASSAAGVEVVAVKTRYPQGSEKHLIKSVLGREVPPGGLPFTVGVVVSNVGTCAAVSNR
                     FDRGLPLIERIVTVTGSAVAKPANLVAKIGTSASDLIEECGGFVGQPGKIVFGGPMMG
                     MAVSSVDVPIGKGTSGILVMSREESEDLKEMPCIKCGQCVDACPMRLEPVFLHQWSNA
                     GLWDEAEAYHAMDCIECGVCTYVCPSRRNLVQAIKRAKFEISARRRAAKERGSK"
     gene            6760..7740
                     /locus_tag="PLM83_02880"
     CDS             6760..7740
                     /locus_tag="PLM83_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOK70386.1"
                     /translation="MGNTKLIVTPAPHIRSGDSTPAIMRDVVIALLPAAISAVYFFGA
                     QAAVSMVCSVAGAVGAEYIFEKIAKKPVTIGDWSAAVTGLLIAMVLPPHVPVWIPVIA
                     GFFAIGVVKLLFGGLGHNVFNPALAGRAFAIAAWPALTTSGYLWPASAEGAIWAVRGF
                     DAMSTATPLTLWKMGAIGGASMSKFYQSLFVGNVAGSLGETSALALLIGAVYLLCRGR
                     ISLRIPLSYILTVAVITGLYGQDPLFHVLSGGLILGAFFMATDYVTSPMNPKGQIIFG
                     IGCGILTALIRMFGGYPEGVCYSILLMNAAVPLIDRLTRPKRFGEVTSRA"
     gene            7733..8320
                     /locus_tag="PLM83_02885"
     CDS             7733..8320
                     /locus_tag="PLM83_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOK70387.1"
                     /translation="MRDALKLGLVLMVICAVSGGLLAYVHGVTSEIIDERKAQEVADA
                     MRVVLPSAESFEKLSDDELASIKADPKFVDVDEVYEGAHDGSLVGIVAKVQSPGYGGK
                     ISLLVGMDPDLTVTGLQVLGHSETPGLGANIAKPEFISEFIGRGGSHPLAVDKDGGEI
                     SAISGATISSRGMVNGVNLVRDLVNALGIAGGVGR"
     gene            8317..8943
                     /locus_tag="PLM83_02890"
     CDS             8317..8943
                     /locus_tag="PLM83_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOK70388.1"
                     /translation="MSPLKAFTNGIYKENPIFKLLLGMCPTIAVSLAVSNGIGMGLAA
                     TFVLVGSNLIISLLRKFIPSEIRIPCFIVVIATFVTIVDLSMEAYLPQLHQVLGVFVP
                     LIVVNCIILARAEAFASKNGPVASIMDGLGMGLGFTLALVVISLIREPLGTGVIFAAA
                     DLGFDGIKLFPAEYAASVVTQPVGGFITLGLLLGLVNLITNKKKDKQG"
     gene            8959..>9434
                     /locus_tag="PLM83_02895"
     CDS             8959..>9434
                     /locus_tag="PLM83_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOK70389.1"
                     /translation="MRQLLVILFSQALVNNFMLSRILGTCPFIGVSQQVETATGMSLA
                     VVFVMTVASAITWLVQNFILNPLNMAYLQTVAFILVIAALVQLIEMVIKKSSPSLYRA
                     LGIYLPLITTNCAVLGVAIINYQANYNLLESAVSGAAGGLGWDLAIILFAAIRERQ"
BASE COUNT         2035 a         2270 c         2893 g         2226 t
ORIGIN      
        1 gatcgtgttc agctgggtat tgcccaggct ttttgccatg tgcgtgacgc gcaactgacg
       61 ccaatttcgt cgtggagcga cacaaaagcc ttattctcat actatatatc agtctgcggg
      121 catagcccgc ggatggaaaa ctttcacggg aggagcgatt gcggtggaaa gcgcagtatt
      181 cgccggcggt ttcaagggtt tcttcgcctg gaacctagca ttgattcttc taggcataat
      241 agtgatcgca gtgttctggt tcccctggta attcaaggca tggcctaaaa aggaggacac
      301 gcacatgaca gttggcgatg ttataaaggc tgctccagac ccagggcctt ttggatgtgg
      361 gtttggatca tggggcatgc tcctaatcct gcttgccata atcgtattgg cagccttcct
      421 gccaagattc tgctggtaga acgaggcagt ctctgcagtc aaccgtgccg attttggcac
      481 ggttgacccc ctgctttcct gtaaaggcca catttcttca cggataaaga ctctgcaaat
      541 tagtgcccat gccatcgttc gtcacaagga cttttgagtt gcccacacaa ttaagccggt
      601 catagctggg taaaccctaa ttggtgatgg gcggtgtgtt atggatgcaa gttgacgcat
      661 cggacgcatc tttggatcat ggatgcgaag cattgttctg caatgattca tactgtatac
      721 tcatggtgtg gtcactcaac atttgaaggg gagtgctggc aatatgaagt cgcttgatga
      781 cctcaagaag atcaaggaac gcgctcaggc aatgacggag ttgcgtgagg gcaacgagga
      841 ggtaaggatc gttattggca tgggcacctg cggtatcgcg gccggcgcgc gcgatacgct
      901 cgcagcggtc gtagatgaga tcaacacgcg caaccttcgg catgtggcag tgacgcagac
      961 gggttgcatc gggatgtgtg aatacgagcc gatcgtggat gtaatcaggc aaggtgagcc
     1021 aaaggtgaca tacgggcgtg ttacgccgga gaaggctcgc cagattatcg ccagtcatgt
     1081 agtgaatggg caggtcgtag gcgacctcgt gatatcctcc aaataggcaa gggctttagc
     1141 gatgggtgcc gatagggaga gcctcacgag gtatttagtt gcggaacttt gtgaaaaaca
     1201 gcacaatctt gaaacgcccg atgttagtaa ggacagaagg ggaggggtcc gagcatgctc
     1261 aaaatgcgcg gtcctgttgg cccggaaggg cctggtgttg tttgtgagtg cgacgacgtc
     1321 ttcttaaccg aagtgcagag ggttgtatct gtctacaagg aacgaccgga agacctagta
     1381 caagctcttc atgatgttca atcgctccga aattaccttc cacgcgaggc tttgcgtgta
     1441 gtagcgcgca ccctcgacgt gcccgaaagc cgagtttatg gagtagctan nnnnnnnnna
     1501 tgttctctac aagtcctagg ggacgccaca taatcaggat ctgcgagtcg gctccgtgcc
     1561 acgtagcggg ggctgctgag ataatcaatt cgctcttgaa cgaactcgga gtggagatgg
     1621 ggggcactac atctgacggg atatttactg tggagacgtc gtcctgtttg ggtgtatgtg
     1681 gtgttgcacc tgccatcatg attgacgaca cagtctacgg taatcttgtc tcggaagatc
     1741 tgcctgcagt tctgcgccgt tatcgttagg tcccggtttt ctgaacgaag cttggggggg
     1801 atccacatgg agttctaccg agcgcatgtc ctggtgtgcg gcggattccc gtgtgtcgcc
     1861 gctggttgcc gggcggtgcg tggtgaaatc gaacgatcgg tcaaggagca tggtcttgag
     1921 cgcgaggtcc gtattgttga gacgggctgc ttaggtccgt gcgaccttgg gccgatcgtt
     1981 gtcgtgtacc cggaaggaac cgtatattcc cgcgtcagcg tggatgatgt aagtgagatc
     2041 gtaggcgaac atctgatgaa ggggaggcct gtcaggcgtc ttgaatacac agagcgtctt
     2101 ccttcggtat cgcaggctga tcacaagcgc cccgactact ttgggatcca agaaaggata
     2161 gtccttgaga atgtggggct gatcgaccca gacagcatcg aggagtatat cgcccgtgat
     2221 gggtatctgg gcgcgggtat ggctcttaca tctatgtcgc ctgatgaagt cattggcgct
     2281 ataaaggctt caggccttcg cggccgtgga ggcgcggctt tccccaccgg cgtgaaactg
     2341 ggattcactg cgcgggtgga ggctgatcag aaatacataa tatgtaatgc cgatgaagga
     2401 gagcccggaa cattcaagga ccgcctcata ctcgagggtg acccgcacaa ggtgatcgaa
     2461 ggcatgatca tcttggggta ctcagttggc gctaccatgg gatacatata catcagaggc
     2521 gaatactatc tctccataga gcgcatagag cgcgcgatcg cggaggcgcg caggtatggg
     2581 ctcttaggtc atgacctatt cggctccggc ttcagtttcg atattgaagt gaagaagggc
     2641 gccggggcgt acgtgtgtgg cgaggagact gcgctgatcg agtcgatgga aggcaagcgg
     2701 ggtgaaccta ggcagaagcc tccttatccg ggaacctgtg gtctgtgggg caagccgacc
     2761 gtagtcaaca atgtagagac catagccaac gtcccgccta tagttcgcaa cggggccgat
     2821 tggttcagac aattcggaac ccctactagc cccggcacaa aggtatacac ccttacagga
     2881 gatgttaaca acaagggact gatagaagtc cccatgggta ttacgctccg tgaggtcata
     2941 tacggggtgg gtggcggaat ccctggggga cgtcgtttca agatggctca gactggcgga
     3001 acagcagggg gctgtctgtc agagacgcac cttgacatcc ctatggatta cgagagtctc
     3061 caggaggcag ggtccgctct cggctccggt gcgctcctga taatcgacga cagccattgc
     3121 gtggtggaca tagcctgcgc gttattgcgc ttttttgagc atgagtcgtg cgggcaatgc
     3181 acgccttgca gagagggcac acgacgactg catgacatct tccgcaagac gcgggagctg
     3241 gccgcaactg aaaaggatct aaagctggca agggagcttg caggggtaat gcaagcctct
     3301 tccctttgcg cgttgggcca atcggttgca gttccgctct ttactatcat ggatcactat
     3361 gcggatgaga tcgaagctca ccttgagggg cgttgccctg cgggcgtatg caggccaaat
     3421 cctgcaaacg cagacgagtc ggcatacgag acgctccagg cgggcaacgg gatcaggtga
     3481 gaggaggctt gacggggaaa tgtctgacat tactttgacc atcgatggca agtctgtctg
     3541 cgcgtcgcct gagatgacaa tcctcgaggc cgcgcgcact gtgggaatca agatcgcaac
     3601 gctctgttgg catgaagact tgggaagccc gtcggtctgc cgcgtctgcg tggtggagat
     3661 agagggccag aacacgctac agccagcctg ctcgtaccct gtaagccagg gtatgacagt
     3721 gcgcaccaat acgccccggg tcagaaatgc cagacgcatg gccatcgaat tgctgcttgc
     3781 gcaccatcct gacgactgcc tttcatgcca gcggaacctc aaatgtgagc tgcaacagct
     3841 tgcggccgat tttgggataa gggagataag gttcgagcgt gtttcaagga agatccccag
     3901 agacgaatct actccttcgc tggtgcgaga tgccgataag tgcattaact gcagaaggtg
     3961 catcgaagct tgtgagaaga ttcagggcgt ggccgtgttg tccactgtca accgcggatt
     4021 tgactcggtg gtgctgcccg ctttcggtga tgatctcgat tctgttgtct gcgtgctttg
     4081 cggacagtgc accttggcct gccctaccgg ggccatagtg gaacgggacg atacgggaaa
     4141 ggtctgggat gcgttggctg accccaatat gcacgtcgtg gtccaaactg ctcctgcaat
     4201 cagggcttct ttgggggagg aactcggact gcctgctggc tctatagtca ctggcaagct
     4261 ggtggctgcc ctcaggaaga tcggttttga cagggtcttt gatactgact ttgctgctga
     4321 cctcacaatc atggaagaag ggcacgagct cctggatcga atacagaagg gcaatgctcc
     4381 gttgccgctc ttgacttcgt gcagccctgg ctggatcaac tttgttgaac gtttctaccc
     4441 tgacctgctt ccgcacgtgt ccacgtgcaa atccccacag cagatgtttg gggccgtggc
     4501 caagacatac tatgccgagc gtgctgacat cgaccctgct cggatcttcg tcgtgtcgat
     4561 aatgccttgc actgcgaaaa agtatgaatg ccagaggccg gagatgagat cgagcgggtt
     4621 tcaagatgta gacgcggtcc ttaccactcg ggaactgggc aggatgatca gggagatcgg
     4681 catagatttc gattccctgc ctgaagagga gtatgatgcc cctatgggga tttctacagg
     4741 tgccggggcc atattcggcg ccactggcgg ggttatggag gcagcgctgc gcacggtgta
     4801 tgccgtggtg actgggcgag agctttccga agatggtctg gaattccatg atgtaagggg
     4861 ccttgagggt atcaaggagg ctgaggttga ccttgaaggc gccaccgtga gggttgcgat
     4921 cgctcacggg ctcggcaatg cgagaaaggt aatggagacg atcaggacgg gtgaagctcc
     4981 gtggcagttt gtggagataa tgtgctgccc gggtgggtgc gtcggaggcg gcgggcagcc
     5041 gatcggaact gtgatgtcga gccgggctga gagaggccaa gctctatacg aggttgacag
     5101 ggagatgccc ttgagggagt ctcacaagaa tcctatatca ggaattcctg gactggccac
     5161 tctcggagcg cagccatgag ctgctgcata cgcattatac tccgaagaaa tcctgaagag
     5221 ttcggttttc ttgcacaagc ttgtttcgtg gtgtacaatt agaccggttt taggcaccgt
     5281 ggctaaagtg ctgttggggt gcgtaaagcc ggtctattca ccggacaggc tttgttattt
     5341 tatgcctcac gtgaggcttt ttgtgctgtg aagttccaag tccataggca ggtttgtaga
     5401 agcaaaagga gtgaggtgca tgcccaaggg atcgttccga gggggagtcc atcctccgga
     5461 gaacaaagag agaacggcca cattgcgcac tgttgttgcc aggcggccgt ctcaagtagc
     5521 aatagcgctt catcagggct ccaactgtcc cttaacgcct acggtcgcca aaggagatta
     5581 cgttcgcatg gggcagaagg ttgccgatac tgatgaaaaa tggggggtac cagcgcattc
     5641 tcccgtctca ggcaaggttg tctccatcgg cctggtgcga accgctagcg gcgtcgagga
     5701 ccaggccata tttattgaac ctgacggaga ggatgcattg gacgaatcgt gtgtgcctgt
     5761 cgacaatgtc ttttcccggt ctcctgaaga gattatcagg ataattcggg atgctggcat
     5821 agtcggaatg ggaggggcgg agtttccaac ccatattaag ctttcgctgc ctccgggggc
     5881 taatgtggat actctgcttt tgaacggcgc tgagtgtgaa ccatacctca cagccgacca
     5941 caggctgatg gtggagcgtc cgcgcgacat tatcagggga gcacagatca tgatgcgtgc
     6001 catcggtgtt tctaggacgc tcataggaat agaagacaac aagcctgatg cgattcgcgc
     6061 catgggggac gctgcttcaa gcgctgcagg cgtagaagtc gtggctgtaa agactagata
     6121 tcctcaaggg tctgaaaagc acctcataaa gtcggtgtta ggtcgggagg tcccacctgg
     6181 aggcctaccg tttactgtgg gagtagttgt cagcaatgtt ggcacttgtg cggcggtgtc
     6241 gaatcgcttc gatcgtggac tcccactgat tgagagaatc gtgactgtga ccggatccgc
     6301 tgtggccaag cctgcgaatt tggttgccaa gatagggact tctgccagcg accttataga
     6361 ggaatgcggc ggttttgttg gccaacctgg aaagatcgtt tttggaggcc caatgatggg
     6421 catggcagtg tcgtcggttg atgttcccat cggcaagggc acatcgggca tcttggtcat
     6481 gagcagggaa gagtcggaag acctaaagga aatgccatgt atcaaatgcg gccaatgcgt
     6541 cgacgcatgt cccatgcgtt tggagccggt gttccttcat caatggagca atgcgggcct
     6601 gtgggacgaa gcagaggctt accatgctat ggattgcatc gaatgtggtg tgtgcactta
     6661 tgtatgccca tcccggcgca acttggtgca ggcgatcaag cgagccaagt tcgagatctc
     6721 ggctcggcgt cgggccgcta aggaacgggg gagtaagtga tgggaaacac taagctcata
     6781 gtgacgccgg cgccgcacat aagatccggg gatagcacgc ctgcaataat gcgtgacgtg
     6841 gtgattgctt tgttgccggc agcgatctct gcagtctact tctttggtgc gcaagcagcc
     6901 gtatctatgg tctgcagtgt tgctggagcg gtgggggcgg aatacatctt tgagaagatc
     6961 gcaaaaaagc cggtgacgat aggggactgg agcgctgctg taacaggcct cttgatcgcg
     7021 atggttcttc cgccgcacgt gcctgtttgg ataccggtca ttgcaggatt cttcgccata
     7081 ggcgtcgtta agctgctttt tggagggcta ggccataatg tattcaaccc ggctctcgca
     7141 ggccgtgcct tcgcgattgc cgcttggcca gctttgacca ctagtggtta tctctggcct
     7201 gcatctgcag aaggcgccat ctgggctgtg aggggctttg acgccatgag caccgcaacc
     7261 ccgctcacac tgtggaaaat gggggcaata ggcggagctt cgatgtcgaa gttctatcaa
     7321 tcgctatttg tgggcaacgt agcgggatca ttgggcgaaa catctgcgct ggcgttgctt
     7381 attggcgcgg tttatcttct ctgtagaggg cgcatatccc tgagaatacc tctttcctat
     7441 atattgacag tagccgtaat cacgggccta tacggtcaag acccgctctt ccacgtgcta
     7501 tcaggcgggc tgatccttgg agcgttcttc atggccactg actacgttac atctccgatg
     7561 aatccaaagg gccagataat cttcggtatc ggatgcggga tcctgacggc gttgataagg
     7621 atgttcggcg ggtatcctga gggtgtctgt tattcgatcc tgctcatgaa tgcggcagtg
     7681 cccctgatcg acaggctcac tcgccctaag cggtttgggg aggtgaccag ccgtgcgtga
     7741 cgcgctcaag ctgggacttg tgttgatggt catatgtgcg gtttctggcg ggcttctcgc
     7801 ctacgtgcat ggtgtgacca gtgagataat tgatgaacga aaggcgcaag aggttgcgga
     7861 cgcaatgcgc gtcgtgcttc cctctgccga gagctttgag aagctcagtg atgacgagct
     7921 tgcgtctatc aaggctgacc cgaagttcgt tgacgttgac gaggtatacg agggagctca
     7981 cgacggcagc cttgttggaa tagtagccaa agtgcagtcg cctggatacg gaggcaagat
     8041 cagccttctc gtgggcatgg atccggacct caccgtcaca ggactgcaag ttcttgggca
     8101 tagtgagact ccgggacttg gcgcgaatat cgccaagcct gagttcatat ctgagtttat
     8161 aggcagaggc ggctctcatc cgcttgctgt cgacaaggac ggcggcgaga tctccgccat
     8221 atcgggagcg actatttcgt cgaggggaat ggtgaacggc gtcaatctgg tccgcgatct
     8281 tgtaaatgcc ctcggcatag ctggaggtgt tggcagatga gtcctcttaa ggccttcacc
     8341 aacggaatat acaaggagaa cccaatattc aaacttctgt taggaatgtg tcctacgata
     8401 gcggtctccc ttgcagtgtc aaacggaata ggtatgggcc ttgctgcaac attcgtcctt
     8461 gtggggtcga atttgataat atcgcttctg cggaagttca taccttccga gattcggata
     8521 ccttgcttca tcgtggtgat cgcgaccttt gtcacgatag tcgacctgag catggaagcc
     8581 tacttgcccc agctccacca ggtgcttggc gtgttcgttc cgttgatcgt ggtcaactgc
     8641 atcattctgg caagggctga ggcttttgcg tctaagaacg ggcccgtggc atcgatcatg
     8701 gatgggctcg gcatggggct cgggtttacg ctcgccctcg tggtgatctc acttatcagg
     8761 gagcctctgg gaacgggcgt catattcgcg gcggctgact tggggtttga cggaataaag
     8821 ctgtttcccg ccgagtatgc ggcgtctgtg gtgacgcaac ccgttggagg cttcatcact
     8881 ctcggtctct tgcttggcct agtcaatctg atcacgaata agaaaaagga caagcaaggc
     8941 tgaaaggggt gtttgtaggt gcgtcagctg ctggtaatct tgtttagcca ggctcttgtg
     9001 aacaacttca tgctctcgag gatcctgggg acatgcccgt tcataggcgt gtcccagcag
     9061 gtagagactg caacaggtat gagcctcgct gtggtgttcg ttatgactgt ggcgtctgcc
     9121 attacatggc tggttcagaa tttcatcctt aacccgctta acatggcata tctccagaca
     9181 gttgcattca tattggtcat tgcggccctt gtgcaactca tagagatggt tataaagaaa
     9241 tccagccctt ctttgtaccg ggctttgggc atatacctcc cgctcatcac tacgaactgt
     9301 gcggtgttgg gcgtggcgat catcaactac caggccaact acaatctgtt ggaatctgca
     9361 gtgagcggcg ccgccggagg gctcgggtgg gatcttgcga ttattctctt tgcggccatc
     9421 agggaaaggc agag
//
LOCUS       DAOSAI010000045         9425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4780_length_9425_cov_3.605870, whole genome shotgun sequence.
ACCESSION   DAOSAI010000045 DAOSAI010000000
VERSION     DAOSAI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4780_length_9425_cov_3.605870"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..782)
                     /locus_tag="PLM83_02900"
     CDS             complement(258..782)
                     /locus_tag="PLM83_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70390.1"
                     /translation="MDDDQTIAKSQKTCKHVTAPTSKQTVSYPQLACNERRLETVVKA
                     IRKDQVDKEELKRLVHSISRVLPPEGEIVTEMGVALGAISIEPDRFGWASCSQYVAIV
                     PTLPALVLSCQHLDRPWSWLVWLWVLGSGAGALGGGQGSAFHGRFSGGRGLCSSILSF
                     VMFGLGVLSVYMNF"
     gene            complement(1152..2384)
                     /locus_tag="PLM83_02905"
     CDS             complement(1152..2384)
                     /locus_tag="PLM83_02905"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOK70391.1"
                     /translation="MRVLFASSEVNPYMKVGGLADVAGSLPGFLAEAGCDVHLVMPKY
                     ETAKWNGIAFQEWGSVYPPMGYGREEAQVYYSKISRNLTLYAVRNGAYFAREKPYDSP
                     DDMRRFMFFAKACYELARALEVDVVHANDWHTGLLPVFCRVYGCPKDPGTVITIHNLA
                     FQGEGDWDDFLYSSLPWDTFNPGGVEYYGKFNILKAAMMYADRITTVSPTYSREIQTP
                     EFGMGLEKVLMARRDAIRGILNGIDYDEWNPMTDALITSNYDVERLDKRIENKRSLAA
                     RFGLEYRPETPVFGFVGRLFEQKGVDILIEATKRLVEQDVQVVVLGTGAPQYEEALRS
                     LSHAHPGKVGAKLAFSNLLAHEIYAGADFFLMPSKFEPCGLGQLIAMRYGAIPIVRAV
                     GGLRDTVTEPVTGLVFWE"
     gene            complement(2463..4052)
                     /locus_tag="PLM83_02910"
     CDS             complement(2463..4052)
                     /locus_tag="PLM83_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOK70392.1"
                     /translation="MARKRPWFVMVLHSHLPWVMGYGRWPFGEEWIYEIAWDCYIPLI
                     RAFRNLERDGIPANITLSVTPVLADQLASESFKSGLVEYLGDRRRRVADDRAEFLARG
                     NTDLARLTIWIEEQIDRALQDFEDMGRDIPGALADLAARGCIELATSAATHGYLPLLK
                     HEKTKRLQIRMGKVGFKEVFGMEPRGFWFPECAYSPGLEELVAGEGYAYTVLDAMAVQ
                     GGKAMSQYGESTRTVPPTGRSVETVYKCCNSELAVFPRHPELCAQVWSRWTGYPGDFA
                     YREFHKQKPRSGMRYYRVTDSAGDLRTKKPYDPTVAAARAREHAAHYVGRIEEAADES
                     RSEVPILTTAFDTELYGHWWHEGPIFLEEVARLLAQSDAVNHATPSRILEEIDWEKVD
                     RIEPLESSWGVNCDHTVWMNERTRPMWEKIWELEDIVNDSWALTPFGPWPGDFWELQS
                     SVDTFLREFLLMMASDWEFLMYTGTAGDYPERRFEGHRKNCAEAYGRLRAALKDHQLP
                     EYLYEPLYDRRDADLESPEGC"
     gene            complement(4100..5302)
                     /locus_tag="PLM83_02915"
     CDS             complement(4100..5302)
                     /locus_tag="PLM83_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOK70393.1"
                     /translation="MGYDFDQVLDRRNTNCEKWDYLEEHFGRADVLPMWVADMDFPSP
                     PEVVQAIQERAKHPSYGYTGTSDSFFDSIVGWMDRRHGWAIEREWISTTPGVVPGLAA
                     SVLAFTEPGDNVIVQSPVYPPFFRVVENNGRRLLNNELVRVGDRYEMDWDDLEAKLGE
                     GAKLFLLCSPANPVGRVWSEEELARISEMCVAHGTVIASDEIHSDIIYSWARHTPFGA
                     LSPEAAENSVTFVAPSKTFNVPGLSTSVAIIPNSDLREGFNRVLSGLGLGTNLFGITA
                     LEAAYTYGDRWLDDAIAYIEENLRFMVEFLGRQVPEIRVVPPEGTYVIWLDCRGLGLD
                     PDALHRFMVEEARVGLSDGTFFGLGGEGYQRINIACPRPILREGLERIASAAVRLRGG
                     VKGTKTGA"
     gene            5435..5830
                     /locus_tag="PLM83_02920"
     CDS             5435..5830
                     /locus_tag="PLM83_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HOK70394.1"
                     /translation="MLEELLSHLEKTSFGIEGFRDHVHRVVGTTVSESEHVHRIDIVT
                     GPPWPGPGGHTHRLLGETDLDDSGHSHQIRGWTRPSSDTSIDHTHQIELQTREIRAHG
                     HKVQGTTASYSPDRWTAMHTRVEDGLRPK"
     gene            6039..7169
                     /locus_tag="PLM83_02925"
     CDS             6039..7169
                     /locus_tag="PLM83_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOK70395.1"
                     /translation="MAEALSEFVMSTGFASLGWQHLVMIAISLILMYLAIEKKFEPML
                     LLPISFGMLLANLPATGLTSPPTGDTPGGLLYYLNFGAETGLYPLLVFFGVGAMTDFG
                     PLLANPSTMLLGAAAQSGIFITFFVSLLLGFEPLESACVGVIGAADGPTSIYITSKFA
                     PHMLGPVAVAAYSYMALVPIIMPPIMRGLTTKEERSVIMTQSRPVSKAERILFPIITT
                     VVSALLLPSTAGLIGSLMIGNLFRESGAVERLNKTAQNELTNIVTIFLGLAVGSSATA
                     EKFLSVKTLQILFLGLFAFSAGIACGVFLGKLMYKLSGGKVNPLIGAAGISAVPMAAR
                     VCQQVGLEEDPNNHLLMHAMGANVAGQIASAVAAGVMMAIFG"
     gene            complement(7128..7460)
                     /locus_tag="PLM83_02930"
     CDS             complement(7128..7460)
                     /locus_tag="PLM83_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOK70396.1"
                     /translation="MQGRGRFHCLSCPVIGSTLAAGADGNLQDEDGWTALMIAAAYCD
                     NPDVILVLLEADADGKLKSKDRMTAFDYARELTCTSSSTRRDSDDSTAAHRLHLAKYR
                     HHDPRGHC"
     gene            complement(7831..>9425)
                     /locus_tag="PLM83_02935"
     CDS             complement(7831..>9425)
                     /locus_tag="PLM83_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOK70397.1"
                     /translation="GMETVLKRVLTAGLVASLLCLAFAASAAASLLPFDPEWTAAEVG
                     YLVAAGAEVNARDENGWTPLMFAAAWADIETVSVLFEAGADVNASSTSGWTALMSAMA
                     CGNWPQVVGEFRLVGLEEDWSWDWGDEYAGDDWGWDDWWDDDDDWEWDDEEDWSWESW
                     YDDDEDWPEEAASPWREPNPLVIRSLVAAGADVNACTEDGWTPLMIAVLCNADCEIVE
                     DLIAAGAEVNARNEFGQTPLILAVENDTDIEVVAELIGAGARVNAKTKDGMTPLMLAV
                     HNDCPDTIRLLVAAGACANAEGKDGGTPLMHAARFNPNPDVIDILIQAGARVNHRGID
                     GLTPLMFATFNVNPDVFSLLIAAGADVNARDDEGWTPLMFAAAGSMNHTAITSLLRAD
                     TNVNARTVDGVTALMIAAAETEDVEVVRTLIAAGADVNAKDMCGWTPLMYAAFNVDPK
                     VIAVLVAAGAHVNDRNVEGFTTLMIAADCCENPEVILALLEAGADGKLKSGEGKTAFD
                     YANDNEIIKGTNVYWLLNDARF"
BASE COUNT         2177 a         2934 c         2210 g         2104 t
ORIGIN      
        1 aaacttcaag tatatctcaa tgtcggcttc aagtcgctgg gcgagattgc cagcgggcag
       61 gaggggagta ttcccttcca gcaacgtcac taccggcatg tctcgttcaa tcgggagata
      121 ctctctatac tcctcaataa tccctctcca tgccctcaaa tagaccacat cctttccacc
      181 gtccgaataa ctacaactcg atgggccgcg ccagcagcca atgccacatt actgcgaagg
      241 cccccacggc ccatctccta gaagttcatg tagacagaaa ggacgcccag cccaaacatg
      301 acaaaactta atatcgagga acagaggccc cgccctcccg aaaaccggcc atggaaggct
      361 gagccttgcc caccacctaa cgcgccggct ccgctcccca gaacccataa ccacacgagc
      421 caagaccaag gacggtcgag gtgttggcag ctcaacacaa gagccggaag cgtcggcact
      481 atggcaacat actgagagca actggcccat ccaaatctgt caggctcgat agaaatagcc
      541 cctaacgcga cacccatctc ggtcacgatt tcaccctctg gaggcaacac cctggatatg
      601 ctgtgcacga gccttttgag ctcctctttg tcaacctggt ctttacggat ggccttgacg
      661 actgtctcaa gacggcgctc gttgcaggca agctgcggat aggaaacggt ctgcttactg
      721 gttggggcgg ttacatgctt gcacgtcttc tggcttttcg cgatggtttg atcgtcatcc
      781 aagctattcg actcttgatt ctgcgcgtgc gcctgttcac tgttggattc ggggttgagt
      841 tgatgggttt gctgccgcta caaatcgagt cttgcccgga ggctcaacag gttaactcac
      901 agcagcgcaa tacgattcgc cgccaccctg cctccgggca agcatttctt atcaaccata
      961 taattggccc ccgtgttgcg gaggaactat cgcccaggcg cgatctcctt gtaaagcgag
     1021 acatactcgt cccgcgacct tccccaggaa taatccgctg tcatgcaacg gatgcgcata
     1081 gcgagaaacg cctcgggatt atcatatatt tccagagccc tttccatggc agcatgcaat
     1141 tctgacgcag actactccca aaagacgagg ccggttacgg gctcagtcac agtatcacgc
     1201 agaccgccca ctgccctcac tatcggaatt gctccatatc tcattgcaat aagctgcccg
     1261 aggccacaag gttcgaattt cgacggcatg aggaagaaat ccgcccctgc gtaaatctcg
     1321 tgagccaaca gattgctgaa cgccagcttc gcgccgacct tcccgggatg cgcatgggat
     1381 aggctcctca gcgcctcttc atactgggga gcacctgttc ccaagacaac tacctgaaca
     1441 tcctgttcca caaggcgctt agtggcctct atcagtatat caacgccctt ctgctcaaag
     1501 agccgaccca caaagccgaa aacgggagtc tcaggcctgt attccaaccc gaatcttgcc
     1561 gcaagggatc tcttgttctc gatccgctta tcgagcctct caacatcgta attggatgtt
     1621 ataagagcgt cagtcatcgg gttccattca tcgtagtcta tgccattgag tatcccccgg
     1681 atcgcgtcgc gcctcgccat cagaaccttt tcaagcccca taccgaactc cggggtctgt
     1741 atctctcttg agtaagtggg agataccgta gttatcctgt cagcatacat catcgcagcc
     1801 ttcaggatat tgaatttccc gtagtattca acgccacccg ggttaaatgt atcccacggg
     1861 agagacgaat agagaaaatc gtcccagtcc ccttctccct gaaacgccag gttatgaatg
     1921 gttatcacag tgccggggtc cttcgggcaa ccgtacaccc ggcagaacac gggcaaaagc
     1981 ccggtatgcc agtcattggc gtgtaccacg tcgacttcaa gggccctggc caattcgtaa
     2041 cacgctttcg cgaaaaacat gaaccttctc atgtcgtcag gggaatcata cggcttttca
     2101 cgggcaaagt aggcaccgtt gcgcaccgca tagagcgtca gattcctaga aatcttcgaa
     2161 taatagacct gtgcttcttc ccgcccatac cccattggag ggtacacaga tccccactct
     2221 tgaaacgcaa tcccgttcca tttggcagtc tcgtacttgg gcattacaag atgaacatca
     2281 catcccgctt cagccagaaa ccctgggagg cttccggcaa cgtcagcaag tcctcctacc
     2341 ttcatatacg ggttgacttc cgaagaagca aagagaactc tcattgctag acagtccccc
     2401 ttcttagtaa ttcgtgttgc atatagtgta gggcaaatca gaggccaacg ccgctgatcc
     2461 ggctaacagc cttcggggga ctcaagatcc gcatcccttc tgtcatacaa gggttcgtaa
     2521 agatactcgg gcaactgatg atctttcaaa gccgcgcgaa gcctgccata ggcctctgcg
     2581 cagtttttcc tgtgcccttc aaagcgccgt tcaggataat cccccgcagt cccagtatac
     2641 ataagaaact cccaatccga agccatcatt agcaggaatt cgcggaggaa tgtatcaacg
     2701 ctggactgta attcccagaa gtcgccgggc cacggcccaa aaggagtcaa tgcccagctg
     2761 tcgtttacta tgtcttcgag ttcccatatc ttttcccaca tcggccgtgt gcgctcattc
     2821 atccacacag tatggtcgca attaacgccc cacgacgatt ccaagggctc tatcctgtca
     2881 accttttccc aatcaatctc ctcaaggatg cgcgacgggg ttgcatgatt caccgcgtca
     2941 ctttgggcaa ggagccgtgc cacctcttcc aggaaaatcg ggccctcatg ccaccagtga
     3001 ccatacagct ccgtgtcgaa cgcggtcgtg agtatgggta cttccgaccg gctctcatca
     3061 gctgcttcct cgatgcgccc cacgtaatgt gcagcatgct ccctggccct ggctgccgca
     3121 accgttgggt cgtatggttt cttagtgcgc aaatcgcctg cggaatcagt cacgcggtag
     3181 tatctcattc cagatcgcgg cttctgcttg tggaattccc gataggcgaa gtctcccggg
     3241 taccccgtcc atctggacca tacctgggcg cacaattccg ggtggcgtgg gaagacggct
     3301 agttcagaat tgcaacactt gtagaccgtc tccacagacc tgcctgtagg cggcaccgtt
     3361 ctggtgcttt cgccgtactg gctcattgcc ttcccgccct gcacagccat ggcatccagc
     3421 acggtataag cgtaaccttc acccgctacg agctcttcca agcccggact gtaggcgcac
     3481 tccggaaacc agaatccccg gggctccatg ccgaagactt ccttaaaccc caccttcccc
     3541 atgcgaatct gaaggcgctt agtcttctca tgtttgagca acggcagata cccgtgggta
     3601 gccgcggatg tcgccagttc aatacagccc cgggcagcaa ggtcggccaa tgcaccggga
     3661 atatctcggc ccatgtcttc gaagtcctgc aaagcccggt ctatctgctc ttcaatccaa
     3721 atcgtcagtc tcgccagatc cgtatttccc cgggcgagga attctgccct gtcgtcagcg
     3781 accctgcgcc gcctgtcgcc caggtattct acgagccccg acttgaaact ctccgatgca
     3841 agctggtccg caaggactgg ggtgacactc agcgtgatgt ttgcgggaat gccgtcgcgc
     3901 tcaagattcc taaacgcccg gatcaacgga atgtagcaat cccacgcaat ctcatagatc
     3961 cactcttcgc caaaaggcca tctgccataa cccatcaccc acggcaaatg actgtggaga
     4021 accatcacaa accagggacg ctttctcgcc accatcttca cctcccggca tgattcacgc
     4081 gtccgcagca tcgagcctcc tacgcccctg tcttcgtgcc cttcactccg ccccgcaatc
     4141 tcactgccgc ggacgcaatt cgctccagcc cttcgcgcag gatcggcctg gggcacgcta
     4201 tgtttatccg ctgatatccc tcgcctccta aaccgaaaaa tgtcccgtcg ctcaggccta
     4261 ctcgcgcctc ctctaccatg aaccgatgca aagcatcggg atcgagcccc agccccctgc
     4321 agtcaagcca tatcacatat gttccctcag ggggaaccac tcggatctcc ggcacctgac
     4381 ggccgaggaa ctccaccatg aacctgaggt tctcctcaat ataggcaatg gcgtcatcca
     4441 gccaacggtc tccgtaggta tacgcagctt ccagggcagt aataccaaac agattcgttc
     4501 cgagtccgag cccgctaagg acgcgattga aaccctccct caagtcggag ttgggtatga
     4561 tcgcaacaga agtcgaaagc cccggaacat tgaaggtctt actgggagcc acaaaggtaa
     4621 cggagttttc agccgcctcc gggctcaggg cgccaaaagg agtgtgtctt gcccatgaat
     4681 aaatgatgtc gctgtgtatc tcgtcagatg cgatcacggt gccatgggcg acacacattt
     4741 ccgaaatgcg cgccagctct tcctctgacc agactctccc cacaggattg gccggactgc
     4801 acaacaggaa aagcttggcc ccttcgccga gcttcgcctc caggtcgtcc cagtccatct
     4861 cgtaacggtc tcccaccctt acgagctcgt tgttgagcag cctcctcccg ttgttctcca
     4921 ccacccgaaa gaacggcgga tataccggag actgcacaat gacgttgtcg cctggctccg
     4981 taaaagcaag gactgatgct gcgagcccag ggaccacccc gggagtagtc gagatccact
     5041 cacgctctat cgcccatccg tgcctcctgt ccatccaccc tactatcgaa tcgaaaaagg
     5101 aatcagaagt ccccgtgtag ccgtatgacg gatgctttgc cctttcctgt atagcctgta
     5161 ccacctctgg gggagaaggg aaatccatat ccgcaaccca catgggcaag acatctgccc
     5221 tgccaaaatg ctcctcgaga taatcccatt tctcgcaatt agtattccgt ctgtcaagca
     5281 cctgatcgaa atcgtatccc atacaaagca ccatcccatc tggaatatct ttgacgcttt
     5341 ggtatttctc ctttccgcgc cattgtcctg tgtgtgccgt gggcacaaca cccctcttgt
     5401 gataagagta taagatgtag cgaggtgatc ggacttgcta gaggagttgc tttctcatct
     5461 ggaaaaaacg agttttggca tagaaggatt tagggatcac gttcacaggg tggtcgggac
     5521 cacggtcagc gagagcgaac acgttcaccg aatcgacata gtgacggggc ctccgtggcc
     5581 agggccggga ggacataccc acaggttgtt gggggaaaca gacctcgatg acagcggaca
     5641 ctctcaccag atccgcggat ggaccagacc ttcctctgac acaagcatcg accataccca
     5701 ccaaatagaa ctgcagacca gagaaatacg agcccacggc cacaaggtgc aaggaaccac
     5761 cgcctcctat tcgcccgatc gctggacagc catgcacacc agggtagagg acggcctgcg
     5821 gccaaagtag aaccgatctt ttggcagaac aggcggtagg aaccgccgcc acgcaccgcc
     5881 aatcacctcc acatgttctc ttgtgaacgc cacggaggtg attggccttt tctgtcgaag
     5941 aataaaaaag aacatgatac ctttgctatc acattcttct ggacagcgct gcagatcggc
     6001 gcagaataca cctgtcaagc tgaaggagga tcctctagtt ggccgaagca ctgagcgagt
     6061 tcgttatgag cacgggtttt gcaagtctcg ggtggcaaca cctggtgatg atcgcgatat
     6121 cgttgatact gatgtacctg gcgatagaga aaaagttcga gcctatgctg cttcttccca
     6181 tatccttcgg gatgttgctg gccaaccttc ccgcaacagg ccttacatct ccgcctacag
     6241 gcgacacccc tggtgggctc ctgtactacc ttaatttcgg cgccgagaca gggctttacc
     6301 ccttgctcgt cttcttcggg gtcggagcga tgaccgactt cgggccactg ctggccaacc
     6361 cctccacaat gctgctgggg gcagcggcgc aatcgggaat attcattacc ttcttcgtat
     6421 cattactgct tgggttcgaa ccgctcgagt cggcatgcgt cggagtgata ggcgccgcag
     6481 acggtccaac ttcgatctac atcacttcca agtttgcccc tcacatgcta ggacccgttg
     6541 ccgtagccgc ctactcctac atggccctcg tgccgatcat catgccgccc ataatgcggg
     6601 gcctcaccac aaaggaagaa cgttcggtca tcatgacaca gtcccgtccc gtatcaaaag
     6661 cggaaaggat actctttccc ataatcacaa ccgtagtttc agcacttctt ctgccgtcca
     6721 ccgcagggct aataggctcg ctcatgatcg gcaacctctt tcgcgaatca ggggcagttg
     6781 aacgcctgaa caaaaccgcc caaaacgaat tgacgaacat agtcacgatc ttcctcggtc
     6841 tggccgtggg atcctcagcc acagccgaaa aattcctgtc agtaaagact ttgcagattc
     6901 tatttctagg cctatttgcc ttctctgccg gcatagcttg cggcgtattc ctgggcaagc
     6961 tcatgtataa actctccgga ggcaaggtca acccgctcat aggcgcagcc ggcatatctg
     7021 cagtcccaat ggccgcccgc gtctgccagc aagtaggact tgaagaagac cccaacaacc
     7081 acctcttgat gcatgccatg ggcgccaacg tagcagggca gatagcatca gcagtggccg
     7141 cgggggtcat gatggcgata tttggctagg tgcaaacgat gcgcagccgt ggaatcgtca
     7201 gaatcgcgcc tcgttgagga gctagtacac gtcagttccc tcgcgtagtc gaaagctgtc
     7261 atcctgtcct ttgacttcag cttgccgtca gcgtccgctt caagcaacac aaggatgaca
     7321 tctgggttat cgcaatatgc ggctgcgatc atcagcgcgg tccagccgtc ttcgtcttgc
     7381 aaattcccgt cggcacctgc ggccaaagta gagcctatca ccgggcaaga taggcaatgg
     7441 aaccggccgc gtccttgcat cctcgtgaga cgccacggag acgatcggtc ttttccgttg
     7501 aagaacaaga agacatgttg attctgctat cccattcttt ccggcagcgt caaagatcgg
     7561 cgcagaagga tcttccctgt cacgctgaag ggggatcctc taagtgagct tcgaaaagtt
     7621 tctgcactat catcgagccc ttgcggatcc gtattctccc caattggccg gtaatggctc
     7681 tgggcaaatg ccaaataccg ctgtaccgcc tggcgctcag ccctagctat ttgggatcac
     7741 ccaagtactt cttcgaacct actcattaca tcgaacatcc cgcatgcgag agaatccatt
     7801 tgctatcaag catgctgtct caatgcatgt tcaaaaccgt gcatcattga gaagccaata
     7861 tacattggtg cctttgatta tctcgttgtc attcgcatag tcgaaggctg tttttccttc
     7921 tcccgacttc aatttgccat cggcacctgc ttcgagcagc gcaaggatga cttcagggtt
     7981 ctcgcagcaa tctgctgcta tcattagcgt cgtgaagccc tccacattcc tgtcatttac
     8041 gtgagcgcct gccgcaacga gcaccgctat caccttcgga tccacgttga aggccgcgta
     8101 cattagcggt gtccacccac acatgtcctt tgcattcacg tcggcgcctg cggctatcaa
     8161 tgtcctgacg acttctacat cctctgtttc ggcagctgca atcatcagag ccgtaacacc
     8221 gtccaccgtt cgtgcattca cgttggtgtc tgctctcaga agcgaagtaa tcgcggtatg
     8281 attcatgctg cctgctgctg caaacattag cggagtccat ccctcatcat cccttgcatt
     8341 cacatcagca ccggccgcta tcagcagact gaatacgtca gggttcacgt tgaaagtcgc
     8401 aaacataagc ggagttagtc catctatccc tctgtgattc acgcgagcac ctgcttggat
     8461 aagtatatca atgacgtcag gatttgggtt aaatctcgcc gcatgcatca acggggtccc
     8521 accatctttg ccttctgcat ttgcacaagc accagcagcc acaagcagcc taatagtatc
     8581 cggacagtca ttatgtaccg caagcatcag aggcgtcatg ccatcttttg tcttcgcgtt
     8641 aaccctagcg cctgcgccga tgagctcagc aacgacttca atgtccgtat cattctctac
     8701 tgcaagtatc aatggcgtct ggccaaattc gtttcttgca ttcacttcgg cgcccgccgc
     8761 aatcaggtcc tcaacgattt cacaatcagc attgcacaat actgcgatca tcagcggcgt
     8821 ccagccatcc tctgtgcacg cgttcacgtc agcacccgca gctaccagcg acctaataac
     8881 caggggattc ggttcgcgcc atggtgaagc tgcctcttct ggccagtctt catcatcgtc
     8941 ataccagctc tcccaagacc agtcctcttc gtcgtcccat tcccaatcgt cgtcatcgtc
     9001 ccaccaatca tcccagcccc agtcatcacc ggcatactcg tcaccccagt cccaagacca
     9061 atcctcttct aggcccacca acctgaattc cccaaccact tgaggccagt tgccacatgc
     9121 cattgcagac atcagtgccg tccaaccact agttgaactg gcatttacat ctgcacccgc
     9181 ctcgaacaac acggaaacgg tctcaatgtc tgcccatgct gctgcaaaca tcaagggcgt
     9241 ccaaccattc tcatcccttg cattaacttc tgcgcccgcc gccactaagt agcccacctc
     9301 agccgcagtc cattcaggat caaacggcaa gagagaagca gcagccgaag cagcaaacgc
     9361 caagcacaag aggcttgcga ccaagccagc ggtcaatacc cgtttcaaca ctgtctccat
     9421 cccta
//
LOCUS       DAOSAI010000046         9297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4871_length_9297_cov_4.488531, whole genome shotgun sequence.
ACCESSION   DAOSAI010000046 DAOSAI010000000
VERSION     DAOSAI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4871_length_9297_cov_4.488531"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PLM83_02940"
     CDS             <1..807
                     /locus_tag="PLM83_02940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOK70398.1"
                     /translation="INRNNRIIDHNHSLTHIVAGNSDDYVGERLSDVFSSHPQVVDFV
                     TNHANGQTNVALQDGSGRVYRVRVRTVQGRRKGAFVKCLTMSDMTREMNVMRDLKDLA
                     HRDALTNIANRRALYCQAEDVLRRCGTGMAVSSIMLDIDDFKQVNDSYGHEAGDMVLK
                     EVALLLKQSIRAQDILARYGGEEFVLIMPDTEVDAAFALAERLRKKIAGAEIFYNDQR
                     IELTVSMGVAGEICSPDLQVDQLIKNADAALYEAKRSGRNKVALYRDCDG"
     gene            1493..3307
                     /locus_tag="PLM83_02945"
     CDS             1493..3307
                     /locus_tag="PLM83_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOK70399.1"
                     /translation="MSLWDRSMCEFMQQVAEAVSTVLQLDVTIVERGLTRVAGTGRYS
                     RLVGQKVASSSVFAESMRTGKPRLITDPRRNVACGKCEVAEKCIETCHMVYPLMMDGE
                     PLGVVGLICFTEDQRRRVVNCSDEYVAFIEQMARLVESGAKNAYVTQEVKKSRDQLTG
                     IVNAVDEGIIAVDDDGIVLCCNRAASKVLGLDGVELVGKKLGDVVADAPILEVVQTRT
                     PYKGKEVIVSVPAGRIQYVSSGSVLRNGGCVVGAVELLTSSKNARRFAYQVSNSQPDQ
                     PIDNILGTAPALMEAKKMALSTAASFSTVLLIGESGTGKELFARAIHYHSARRYGPFV
                     TVNCAAMPEELLDSELFGYEEGAFTGARKGGKPGKFELADEGTLFLDEVADCSLRLQA
                     KLLRALDDGEICRIGGTQTLTSNARIIAATNRNLEQMVRQREFREDLYFRLNVIPIRI
                     PPLRERKTDIIPLFSYFLNKHAILSQRDIPVLCEEVEQILLVYDWPGNIRQLENATQY
                     VLHTCLGHTVEPHHLPAQVTKGTMEHGGSSTGDIANETGSQAFNWFVSKSEWERMAIA
                     EGLRQLGHSGKAKETIAERLGISRATVYRRIREYDLGG"
     gene            3534..9266
                     /locus_tag="PLM83_02950"
     CDS             3534..9266
                     /locus_tag="PLM83_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOK70400.1"
                     /translation="MAKARRPNWASMAIIMVIIVFFAASVESASTASPVDAGNAWAQQ
                     ELAGEAFEDGEYLVRFIDGVGSSEVTGSLDRIGMKIVKPIWFKPSDAFPQGLTIYRVK
                     VCDGQRINAESVVEKLGANPQVMYVSPQRKVYAYDSPSAVIPDDPLFPRMWGLNNTGQ
                     ESYPGLVGTPDADIDAPEAWAIRHDVPEIVVAVLDTGIEWDHPDLMDNIWINSGEIPG
                     NGADDDGNGYIDDIYGWDFANDDNSVYDGLDSHGTHTAGTIGAVGNNGLGVTGIAWNT
                     KIMCLKFLEDGVGDTGNAIEALAYAWNNGAHITSNSWGYSGPLDPALEDAFRACDLLH
                     ICAAGNTGSDNDSKYPKQSHYPSSFPLDNIIAVAATDCNDRLAAFSCWGKTTVDIAAP
                     GHRILSTLPTDQTPEGYLPYAWMNGTSMATPHVAGACAVLMAEFPDMLFYPWGSDWQD
                     DQVTIKELILSTADKKPDLEGVVLSGGRLNICNALRQQVPPKIANVSGFPPYGSPPLA
                     VTFAATVEDEANILDAWWDFGDGSEAVHAYTASHVYGQTGMYTSTFYAIGRNGIGANA
                     SVRVTVYDEGSVIFVDDDGGASDSEVFLNALNGIGVPWVLVEPPVPFSPDISNPVIWT
                     TGRTMQRTLTSEDQIWLSQYLDNGGRLFLCGQDIIWDLGRDNAFVQQYLHVAEYVKDV
                     RTDNVYGIPDDPLTDGMVIKLDYPFKDYSDTVLPDKYATPIFSNDSGRPCALKYYGPD
                     TYRLVFFVFPFEAIPDIKWTDGENADTGIEATATDVMAAIYEFISEEGDPKASVCPES
                     FDITLARGSSASEVLAISNTGESRFFFELGIDRSDAGNPGGDGPFGRRDTYGYIWRDS
                     DSPEGPEFKWVDITDRGTLGVLGDDYSIDIELPFEFMFYGEAKNRVTISADGFLTFGV
                     TGSAAVNTALPDRALPNDLIAVFWDDLQVTDGGVYYYYDAANERFIVSWVGAHRIENK
                     VPEGCFNFQAILYRNGKIVYQYASMDGTMTNATVGIEDRSGEVGLQVICNAEYLHSNM
                     AVELIHEADGISFGRRIGAVRPGEMIDIEVVIDAQMLDEGKYARQILVNIDDPVNPVI
                     AVPVNIAVTGNELPVVTKATVTPDHGEPPLTVMLEAGAYDSDGELADLWWDFGDGTPP
                     EHDLQTTHVYENPGFYEASFHAVDNLGASTSVTVPVRVEGAAYASVKPDALVVSVIPH
                     STATRRLEIQNSGTSPLRFMVTGFKTNEANFTGYERKAVLDGWDPNAYDSGDQFVIED
                     RTITKLDIGDKVQSWPINGVFRAWGCGYDGENVWFTDLRKRDWQFTTEGEKTGVTFEL
                     PWVGQWAADLAWDGEYLWHVNVGGDNRIYCIDPADGEVVTSIRDPYGIWDATSQRGIA
                     YDPVEDVFYIGGWNQLVIYKIAGLSWPRPGEILRTYDDSTLPVAGIARVGRYLVLTLN
                     GRPDMVAIMDSSTGEMLTWFQHPSGSRNKAGGCEADEHGNVWMTDLNNTAYLLDMGFL
                     LEDMPVWLSADPVEDIVEPGAASHVTLAFDTDRMSVGDVCSTSIAVTSNALDNPLIVV
                     PVTLRVDGLRASFEWREDEPNTVTFTDKSVDEDGGEIVSWLWDFGDGGSSSEQNPVHT
                     YAMNHTYSVTLTVTDSDGAVASDTRQVVLVNAEPVVDFEYRFVEDLAVHFIDKSVDPD
                     GIISSWAWDFGDGTVSSEQHPIHVYSELREYEVTLTVVDNDGAPATIARTMKPDMLPR
                     VTLLAPVGGELWVGKHEIVWIATDVDDPDDTLTIKIEQSSDGGESWMTIADGENNEGL
                     YIWDTTLLNKGGCYIVRITATDPKGGAGTDTSEEFTIIVISRSVVAAPNPAEDNVIFY
                     YDIEADGRLYVYDVAGRLVYEAELPAAANAHHWNLMAGDRPISNGLYLYVLVTETGER
                     SEVGRLVIQR"
BASE COUNT         2302 a         2099 c         2696 g         2200 t
ORIGIN      
        1 atcaacagaa acaatcgcat tatcgaccat aaccattcct tgacgcatat tgtggcaggc
       61 aattcggacg attacgtcgg ggagcgcctg tctgacgtct tcagttccca ccctcaggta
      121 gtcgattttg tgacgaacca tgcaaatggc cagaccaatg tggctcttca agatggttcc
      181 ggccgcgtgt acagagtcag agtccgcacg gtgcagggca ggagaaaggg cgcatttgtg
      241 aagtgtctga ctatgagcga catgactcga gagatgaacg tgatgaggga cttaaaagac
      301 cttgctcatc gtgatgccct cactaatatt gcgaacagaa gggcgctcta ctgccaggcg
      361 gaagatgtat tgaggcggtg cgggactggc atggccgtct cttcaatcat gttggatatc
      421 gacgacttca agcaggttaa tgatagctac ggacatgagg cgggagacat ggtcctgaag
      481 gaagtggctt tgcttctgaa gcagagtata cgggctcagg atattctggc tcgttacggg
      541 ggcgaggagt ttgtgttgat aatgccggac accgaagtgg atgccgcctt tgcgctggcg
      601 gaaaggttgc gaaagaagat agctggcgct gagatattct acaatgatca gaggattgag
      661 cttactgtca gcatgggggt agcaggcgaa atctgctcgc cagatctgca agtcgatcag
      721 cttatcaaga atgcagatgc cgcgctttat gaggccaagc gctccggacg gaacaaagtt
      781 gcgctctatc gtgattgtga tggataaagc cggtgttctg ccatgatggc attgatcttg
      841 ccagcgattt ggggtgagag gcatatgatg tcctcgcctt cgagatagtg taccactggg
      901 tctgtatggt tgtgagaagg aggaaggcca aatcgcagca ttgagcgaag acgcgcgttg
      961 ccatccgccg cctgggccga catccccaaa actgcgattg acaattgttc tgggtatggt
     1021 tagctcccag acgatccaac cataggcatc aaacccatac aatgcttcac tgcatcctaa
     1081 ggcctggtaa aaggtgcatc aaccccacat tgttgtgagg caatggtgac cgatttcaaa
     1141 gggattccag attgttctga cgaatagatt agagaaatgc cttatctggg ggaagccccg
     1201 agcttctccg atcatgcagg ccatgccata agaagtggga gggcttcgac gggtcgacat
     1261 gggactgttt gctatgactg aaatgcgcaa cataccgacg cactacctaa tggaccagat
     1321 gttgttggct tcaacatcgc aatattgatc tagcacattc gcccacattg cctgccttcg
     1381 ccgagctagg gaacttgcag cagacatgtt gcatcatcat tacagctgac tggggtgtga
     1441 tgagcatgca caaggactgc gactaaagat agattccaag ggtggttgcg tgatgtcatt
     1501 gtgggacaga tcgatgtgcg agtttatgca gcaagtagct gaggcagtgt ctactgtttt
     1561 gcagttggat gtcactatag ttgaacgcgg gttgacgcgg gttgccggca ctggacgata
     1621 ctccagactt gttggtcaga aggttgcctc gtcgtccgtt tttgccgagt caatgcgaac
     1681 tggcaaacct cgcttgatca cggacccgag gcggaacgtc gcctgcggga agtgcgaggt
     1741 cgcagagaaa tgtatcgaga catgtcacat ggtttatcct ctcatgatgg atggcgaacc
     1801 tttaggggtg gtaggcctca tatgcttcac ggaagatcag cgccggcgtg ttgtgaactg
     1861 ttctgacgag tatgtggcat ttatagaaca gatggccagg ctcgttgaaa gcggtgcgaa
     1921 gaatgcctac gttacacaag aagtcaagaa atctcgtgac cagcttactg gtatagtcaa
     1981 tgctgttgat gaaggcataa ttgcagtaga cgacgacgga attgttctct gctgcaatcg
     2041 agcagcatca aaggtgcttg gcttagacgg agtcgagctt gtaggaaaaa aactcggtga
     2101 cgtggttgct gatgcaccca tactggaagt tgtgcaaacc cggacgccat ataaagggaa
     2161 agaggtcata gtctcggtcc cggcggggcg aatccagtat gtcagcagtg gatcagttct
     2221 gcgcaacgga ggttgtgtcg tgggcgctgt ggagttgctc accagctcca aaaatgcaag
     2281 acgttttgca taccaagttt caaatagtca gccagaccag cccatcgaca atatacttgg
     2341 cacggcgcct gcactcatgg aggctaagaa gatggccctc agtacggcgg ccagcttctc
     2401 cacagtcttg ctgattggcg agagcggcac aggtaaggaa ctctttgctc gcgctattca
     2461 ttatcacagc gctcgacggt atggaccatt tgtcacagtg aattgtgctg ccatgcccga
     2521 ggagctgctc gattcggagc tgtttggtta cgaagaaggt gcgtttaccg gcgcccgcaa
     2581 agggggcaag cccggcaagt ttgagctcgc agacgaagga accttgttcc tcgacgaggt
     2641 tgcagattgc tcgctgaggc ttcaggcgaa actccttcgt gcactggacg atggagagat
     2701 ttgtcgtatc ggtggaacac agactctgac ttcaaatgcg cgaatcattg ctgcaaccaa
     2761 tagaaatctc gaacaaatgg ttcgacagcg cgagttccga gaggacttgt atttccgctt
     2821 gaacgttatt cccatccgca ttccgcctct aagagaacgt aagaccgata ttattcccct
     2881 cttcagttat ttcctgaaca agcatgctat tttatcgcag cgggacattc ctgtgctttg
     2941 cgaggaggtc gagcagattc tgcttgtcta tgattggccg ggcaacataa ggcaattgga
     3001 aaacgcgact caatatgttc tccatacgtg cctaggacac actgtagaac ctcaccacct
     3061 cccggcacag gtgaccaaag gcacaatgga acatggaggc tcgtcgactg gtgacatagc
     3121 caatgaaaca ggaagtcagg ccttcaactg gttcgtatca aaatcggaat gggaacggat
     3181 ggctatagcc gagggcttga ggcagctagg gcatagtggc aaagcaaagg agactatcgc
     3241 ggaacgactg ggtatcagtc gtgccactgt atatagaagg atccgtgagt atgatctggg
     3301 cggataactg tcaagcataa agtagccagc tatctcccgt taattgtctg cctgttttgt
     3361 tgccttagcg tggtctcgag gtgagaccaa tctcagattg agattagggc acgtgccatc
     3421 cccctctggc ttctgccagc ggatacgtgt tgatcaaatc atggcacaga tattgcaaat
     3481 aaatctgagg tactggtttg tgattattat ctgaatgaag tggagggaga ttagtggcca
     3541 aggctaggcg cccaaattgg gcaagtatgg caattatcat ggtcatcatt gtattctttg
     3601 cggcttcggt cgagtcggca tctacagcta gcccggtaga tgctgggaat gcctgggccc
     3661 agcaggaact ggctggggag gcgtttgagg atggcgaata tctggttaga ttcattgacg
     3721 gtgtaggttc ttcggaagtt acgggcagct tggaccgtat cgggatgaag attgtaaagc
     3781 cgatatggtt caagccgagt gatgcatttc ctcaaggact gactatttat cgggtgaagg
     3841 tctgtgatgg ccagagaatc aatgcggaaa gcgtggtcga aaaactcggg gcaaacccgc
     3901 aagtaatgta cgtatcgcca cagagaaagg tttatgcgta cgattcgcca tcagcagtga
     3961 tcccggacga tcccctgttt cccaggatgt ggggcctgaa caataccggt caagaatcgt
     4021 acccggggct cgtcggtact cctgatgctg acatagatgc gcctgaggcg tgggccatca
     4081 ggcacgatgt gccagaaatc gttgttgccg ttctcgatac cgggatagag tgggatcatc
     4141 cggatcttat ggataatata tggattaatt ccggggagat cccgggcaat ggtgccgatg
     4201 atgacggaaa cgggtatatt gatgatattt atggatggga tttcgcaaac gatgacaaca
     4261 gcgtgtatga tggcttggat tcacatggaa cgcataccgc gggaaccata ggcgctgtag
     4321 gcaacaatgg ccttggagtg accggaattg cctggaatac caagattatg tgtttgaagt
     4381 ttcttgaaga tggtgtcggg gacactggta atgctattga agctctagcg tatgcttgga
     4441 acaacggagc gcatatcacc tcgaattcct gggggtacag cgggcccctc gatccagcgc
     4501 tcgaggatgc cttcagggcc tgtgatttgc tccatatatg cgctgcggga aatactggga
     4561 gcgataatga ttctaaatat ccaaaacaaa gccattaccc gtccagtttt ccactggata
     4621 acataatcgc ggtcgccgca accgactgca atgataggct ggcggctttt tcgtgttggg
     4681 gaaagaccac agtggatatt gccgctccgg gccaccgtat tctttcgaca ctgcctacag
     4741 accaaacccc ggagggatat ctgccatatg catggatgaa tggcacatcc atggcgactc
     4801 cacacgttgc gggagcctgc gcagtgctaa tggccgagtt tccggatatg cttttttacc
     4861 cttggggtag tgattggcag gatgaccagg tgacaataaa agaactcatt ctgtcgactg
     4921 ctgacaagaa gcccgatctc gagggcgtag ttctatctgg tgggcggcta aacatctgca
     4981 acgccctgcg acagcaggtt ccgccgaaga tcgctaatgt aagtggtttt cctccctatg
     5041 ggagccctcc attggcggtt acctttgcgg ctacggtgga agatgaagcg aatattttgg
     5101 atgcgtggtg ggatttcggg gatggcagcg aagcggtgca tgcatatacg gccagtcatg
     5161 tatatggtca gacgggcatg tatacttcaa ctttctacgc catagggaga aatggtattg
     5221 gggcaaatgc gagcgtgagg gttaccgtgt atgacgaggg ctccgtcatc tttgtggacg
     5281 atgatggtgg ggcgagcgat agcgaggtat tcctcaatgc tttgaatggc attggagtgc
     5341 catgggtatt agtggaacca cctgtgccat tttcccctga tatcagcaat cctgtgatat
     5401 ggaccaccgg cagaacaatg cagcggacat tgacctccga ggatcaaatc tggctttcgc
     5461 agtatcttga taacggcggc agattattcc tatgcggtca ggacatcatc tgggacttag
     5521 gtcgtgataa tgccttcgtc cagcagtacc tacacgtggc cgagtacgtg aaggatgtta
     5581 gaacagacaa cgtgtacggc attccggatg atccgctcac tgatgggatg gttatcaagc
     5641 tggattaccc gttcaaagac tacagcgaca cagtacttcc tgataaatac gcaaccccga
     5701 tattcagtaa cgattcgggg cgcccctgtg cgctgaagta ctatggaccg gatacgtaca
     5761 ggttggtttt cttcgtcttc ccgtttgagg cgattcctga tatcaaatgg accgacggag
     5821 agaacgcaga caccgggatt gaggcgactg cgacagacgt catggcggca atatatgagt
     5881 ttatctctga agagggagat ccgaaggcgt ctgtttgccc cgagtctttc gatataaccc
     5941 tcgcccgcgg gtcatcagcg tctgaggtcc ttgccatatc caatacggga gaatcgaggt
     6001 tcttcttcga gcttggcatt gaccgatcgg acgctggcaa tccgggcggc gatgggccat
     6061 tcggcagacg cgatacgtat ggctacatat ggagagatag cgatagcccg gagggacccg
     6121 agttcaaatg ggttgacatc acggatcggg gtaccctcgg cgtactgggt gatgattact
     6181 ccatagatat cgagttgccg ttcgaattca tgttttatgg cgaagcgaag aatagggtta
     6241 cgatttccgc tgatgggttc ctcacttttg gcgtcacagg ttccgcagct gtgaacacgg
     6301 ccctgcccga tcgtgccctt cccaatgatc taatagctgt tttctgggat gatctgcagg
     6361 ttactgatgg cggggtctat tactattacg acgctgcaaa tgaaagattc attgtgtcgt
     6421 gggtgggcgc ccatcgcatc gaaaacaagg tcccagaagg ttgcttcaac ttccaggcaa
     6481 tactgtaccg caacgggaag attgtgtacc agtatgccag catggatgga accatgacca
     6541 atgccacagt gggcattgaa gacagatctg gcgaagtggg cctccaagtc atctgcaatg
     6601 ccgagtacct ccatagcaac atggcagtgg agctgatcca cgaggcagat ggcatttcgt
     6661 tcggtagaag gattggcgcg gtgaggcccg gtgagatgat cgacatagaa gtagtcatcg
     6721 acgcccagat gctagatgaa ggcaaatacg cgcggcagat attggtgaac atcgacgatc
     6781 ctgtcaaccc ggtcatagcc gtgccggtga acatcgcagt gacaggcaac gagttgcctg
     6841 ttgtaacaaa ggccacggtc actcccgatc atggcgaacc gcccctaacg gtgatgcttg
     6901 aagcgggagc gtatgactca gatggagaac tggccgattt gtggtgggat tttggagatg
     6961 ggacccctcc cgagcacgat ctccagacca ctcatgttta tgagaatcct ggattctatg
     7021 aagcctcctt ccatgccgtg gacaacctgg gagcaagcac ttcagttaca gtgccagtca
     7081 gggttgaggg ggctgcttat gccagtgtca aacctgatgc tctggtcgtt tcagtaatcc
     7141 cacacagcac agcgacacgt cgactggaaa tacaaaatag cgggacttcg ccattgagat
     7201 tcatggtgac gggttttaag acgaacgaag ccaatttcac gggctacgaa agaaaggcag
     7261 ttttggatgg ttgggatccg aatgcgtatg attctgggga tcagtttgtt attgaagaca
     7321 gaaccatcac aaaactggat attggcgata aggtgcaatc atggccgata aatggagtat
     7381 tcagagcatg gggttgtgga tacgatgggg agaacgtgtg gttcactgat ctcagaaagc
     7441 gcgattggca attcactacc gagggagaga agacaggcgt aactttcgag ttgccctggg
     7501 taggacaatg ggcggccgac ctcgcttggg atggagaata tctctggcac gtcaatgtgg
     7561 gaggcgataa caggatatac tgcatagacc ctgccgatgg cgaagtggtc acatcaataa
     7621 gggatcccta cggtatttgg gatgccacct cccagcgggg aattgcatac gatcctgtgg
     7681 aggacgtgtt ctacatcggc gggtggaacc agctggtaat ctacaaaatt gcggggcttt
     7741 catggcccag acccggtgag attcttcgga cgtatgacga tagtactctg ccagtggcag
     7801 gcatcgcacg agtcggcagg tacttggtct tgacgctaaa tggtcgaccg gacatggtgg
     7861 ccatcatgga ttccagcact ggtgaaatgc tcacttggtt tcagcatccg agtggcagca
     7921 ggaacaaagc tggcggctgc gaggcagacg agcacggcaa cgtttggatg actgatctaa
     7981 acaatacggc atatctcctg gatatgggtt tcttactaga agacatgccc gtgtggctga
     8041 gcgccgatcc ggtcgaagat attgtggagc ccggagcagc ttcacacgtt actctggcat
     8101 ttgacacaga tagaatgagt gtgggcgatg tttgcagcac gagcattgcc gtaaccagca
     8161 acgctttgga caatcccctc atagtggttc ctgtgacctt acgagtggac ggactgaggg
     8221 cgagttttga atggcgcgaa gatgaaccca atacggttac gttcacagac aaatcagtgg
     8281 atgaagatgg cggggagatc gtcagttggc tatgggattt tggagacggc ggatcttcgt
     8341 ctgaacagaa tccggttcat acctatgcga tgaaccacac gtactctgta accctcacag
     8401 tgacagattc cgatggagca gtggccagtg atactcggca ggttgttttg gtcaatgctg
     8461 aaccggtggt cgattttgaa tacaggttcg tggaagattt agcggttcat ttcatcgaca
     8521 aatcggtcga tccggacggg ataataagca gctgggcatg ggattttggc gatgggactg
     8581 tgtcttcaga gcaacatccc atccatgttt atagtgagct tagagaatac gaagtcaccc
     8641 ttaccgtggt cgataatgat ggagcgccag ctaccatcgc aaggaccatg aagccggaca
     8701 tgcttccacg ggttacactg cttgcgcccg ttggaggaga attatgggtg ggcaagcacg
     8761 agatcgtatg gatagctact gatgtagatg atcctgatga tactctcaca atcaagatcg
     8821 aacagtcttc tgacggcggc gaatcatgga tgacaatcgc tgatggagag aacaacgagg
     8881 gcttatacat atgggacacc acacttttga ataagggtgg gtgctatatt gtgcggatta
     8941 ctgctactga tcccaagggc ggagcaggaa ccgatacaag tgaggagttc acaatcattg
     9001 tgatatcgcg gtctgttgtg gctgcgccaa acccggcaga agacaatgtg atcttctatt
     9061 acgatatcga ggcagatgga aggctatacg tatatgatgt agccggaaga ctcgtctatg
     9121 aggcagaatt gccagcagcc gctaacgcgc accattggaa cctcatggcg ggggataggc
     9181 caatttccaa tgggctgtat ctctatgtgt tggtgactga gacaggcgag aggtccgaag
     9241 tgggacggct ggtcatacag aggtagacaa ctcaaaaagc aatgaatgtg cctctta
//
LOCUS       DAOSAI010000047         9290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4874_length_9290_cov_4.533730, whole genome shotgun sequence.
ACCESSION   DAOSAI010000047 DAOSAI010000000
VERSION     DAOSAI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4874_length_9290_cov_4.533730"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..882
                     /locus_tag="PLM83_02955"
     CDS             307..882
                     /locus_tag="PLM83_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOK70401.1"
                     /translation="MAPQNGSDEPVKPAPSGAAAAAAVQSPIEFSATPVAQNWYENAI
                     CILRNSGCKLTEQRRLILLLFEHGPGHLTAQQVFESVTRSQPDISLVTVYRTLELLKD
                     VGVLSKINFNDGADRYERNLGNAGHHHHLICTGCGRVVEFGDCEISSLARKLEQATGF
                     TIDSHWLKLTGQCATCRLAAPASTPGTNKEE"
     gene            886..1692
                     /locus_tag="PLM83_02960"
     CDS             886..1692
                     /locus_tag="PLM83_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOK70402.1"
                     /translation="MSHLHIPDGVLPLPWILAGFGATAILLLFAVSSIRRQDRARTVP
                     RIAILSAVMLLAMSLPIPVLHYHVNFTVLTGMMAGPAEGFIAGFITNLILALGAHGGI
                     TVIGLNTLITGLEITLGWLLWSLFGSRKGRPFVKTAIITIIVLAVSTTAMLGIVAGTE
                     INPSLVMHHHHTHTVHEEEEPFDEADHLLPDHDSHEHGPDSDTRLASFKTFATIVYTA
                     GIVGWAIEAVITGSVVQFISESRPDLIGMDTLPVPGDDDDEEEPRHTGEA"
     gene            1695..2399
                     /locus_tag="PLM83_02965"
     CDS             1695..2399
                     /locus_tag="PLM83_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOK70403.1"
                     /translation="MDIRFIDELAANGRTPLHAALPWAKVLASALLLTSTIASSSPAM
                     AGLVAGAVVLLACATKQPLWYTARIAIYPVVFAALFAIGSVWSNPLYAITIVTKAFTA
                     GLIAATLITTTSFVDVFSIISHVLPSLVADALFMGYRAFFILVDELQGLMVAVRLRGG
                     VSGTLPQQLRTYGQMLGVLVIHSADMTERMYRVMQVRGYSQRISATSEHRPPNAMDFA
                     LLAYSALTLVGVFLYR"
     gene            2396..3235
                     /locus_tag="PLM83_02970"
     CDS             2396..3235
                     /locus_tag="PLM83_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HOK70404.1"
                     /translation="MSDEIIAEIKCLRHVYPDQTEVDLCGLDFSVRRGQRVAVLGANG
                     SGKSTLIFHLLGLLAPVDGKVSVFGLSPSKQFKEMRNRVGVVLQNVDDQIIGPTVWDD
                     VTFTPRALGWPRDEIEAKGKAILNRLGIWHLRRKVPHYLSGGEKRKVALAGALITEPE
                     LLIMDEPFTGLDPRSRTELASLLAQLHAESGITYIVSTHEIDYVPAIADWVYVLAGQG
                     KMALSGTPEEVFAQTDALRDAGIEPPVLADLFESLKKRGWDVSVPLTVDQALEELDYA
                     RVR"
     gene            complement(3330..4979)
                     /gene="hutU"
                     /locus_tag="PLM83_02975"
     CDS             complement(3330..4979)
                     /gene="hutU"
                     /locus_tag="PLM83_02975"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOK70405.1"
                     /translation="MARVIRAPRGTELSCKGWQQEAAMRMLMNNLDPEVAERPEDLIV
                     YGGAGKAARNWECFDAIVDSLKELEGDETLLVQSGKPVGVFRTHPDAPRVLISNAMLV
                     PHWATWENFWELEAKGLTMYGQMTAGSWIYIGTQGILQGTYETFAAAAKKHFGGSLKG
                     RLILTAGLGGMGGAQPLAATFNDAVVIAVEVDRERIERRLKTDYCEMVTDSIDEAIAL
                     AREAQEKGIPRSIALLGNAAETHPEFVRRGIIPDLVTDQTSAHDPLNGYIPAGLTLEE
                     AGELRKSNPEEYKRRAMESMAIQVRAMLDMKDRGSVVFDYGNNIRQRAFDAGVKDAFD
                     FPGFVPAYVRPLFCEGKGPFRWVALSGDPEDIYVTDEVIMEHFAYDEHLVKWIQMAHD
                     KVAFQGLPSRICWLGYGERARFGLMINDLVAQGKIKAPIVIGRDHLDCGSVASPNRET
                     EGMKDGSDAIADWPVLNALLNAVNGATWVSFHHGGGVGIGYSLHAGMVVVADGTPEAA
                     VRLERVLTGDPGTGVMRHADAGYEKAQAFAAGHGIKIPKIG"
     gene            complement(5053..6582)
                     /gene="hutH"
                     /locus_tag="PLM83_02980"
     CDS             complement(5053..6582)
                     /gene="hutH"
                     /locus_tag="PLM83_02980"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOK70406.1"
                     /translation="MVHVAVKGKDLTVREVVAVAREGATVSLSSEARFRIAEASGFVE
                     SLLERDRPVYGVTTGFGKFADVVISPQDAEELQRNLLLSHASGVGETLPREVVRGILL
                     LRANALACGYSGVRVEVVETLLSMLNAGIHPVVPSQGSVGSSGDLAPLAHTMLVMIGE
                     GEAEYEGQVMDGASAMSKARIPIVALRAKEGLALINGTQVMTSIGALAVWDVARLAKV
                     ADIAAAMSAEALRGTTAAYDSRVHAVRPHPGQAASARNLLRLMEGSGIRASHMDCPKV
                     QDAYSLRCAPQVHGAARDAIDYALRAVETEMNSVTDNPIIFADDGDVISGGNFHGEPM
                     AMAMDFLGIAASELASISERRTERMVNPQLSELPPFLTEHGGLNSGMMIAQYTAAALV
                     SENKILASPAVVDSIPTSGNQEDHVSMGTIAARKARQIATNVANVLAIEIVCAAQGID
                     FRAPLEPGTGTRAAHRAVRERVAHLDADRILAVDIARVREMIESGDLLREVEKAVGEL
                     E"
     gene            complement(7023..8507)
                     /locus_tag="PLM83_02985"
     CDS             complement(7023..8507)
                     /locus_tag="PLM83_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOK70407.1"
                     /translation="MSEPDSGRLPRVYWGTAIAAGAFAVARFVPLPDVVAASGEILSL
                     TATGRSALGIVMFTAILWALEVFPFPVTALLGAVMLPLFGVMGWTDVIKSGAGSDIVP
                     FLLGIMILSTGVVESGLALRVSKRIVRMAGSDPKRVVLAFLLFSGFCAAWLGNLAVAA
                     ILQPIAVAIIVSNGLVPGHSNFGTALSIGCAWGALIGSIATPAGGASNLIAIGFLRNL
                     AGVDLGFTQWMAVGFPAAMLMMAPAWAILIMAFPPEIEELRVDSADADDGECGSEGKS
                     RGAIVANGSPLTAHEIWALVGLGVMLTLWFLSDWLKKAFGMDMPMSLGAIIGAVVYLL
                     PVNGTSSWDVLEHKINWGNLVLVAVGMSLGAGVYDSGAAAWLANAVFGRMAALQPFPR
                     ALLLSTAIMLFKLMFSSNTATASIVMPVVLAAINGAAVDPWTFLAPAALASPLAFILV
                     TSAPANLVFHDSECFSVRDMAVVGIPMTIVAAICIAFVCTLVLG"
     gene            complement(8698..>9290)
                     /locus_tag="PLM83_02990"
     CDS             complement(8698..>9290)
                     /locus_tag="PLM83_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HOK70408.1"
                     /translation="YATKNEMFNEIVVALGGRVAEEIVLGEISTGAQNDLERASKTAR
                     KMVMEYGMSDLGPITYGRSHEEQIFLGRDIARDRNYSEEVASRIDAEVHRMVTEAYNK
                     ATEMLTQNMDKLHLLATALKERETIEGEELDALLSDDNEAQDSDYGADKSDAGLGSAA
                     VVGGSQPEEAKSGIAAIEERLRAGKQRDPGPEGAPA"
BASE COUNT         2087 a         2829 c         2376 g         1998 t
ORIGIN      
        1 cgaaaaaggc agttcaacgc gtgccaataa gctgcttagg gctcaacgat aaagcgacct
       61 caaccgcgag acatacctcc tgaagcaata agcaattctc aatagcatcc gacgcgccgt
      121 ctgcaccctc tgcccgcagt atcaatacaa cagctctgcg ggatttacat atcaatgcac
      181 actcaccaca aaggtgagac attgcacttg gcttatcttc tattgcgctt ggcaggggga
      241 gaagagtata atgtgagaga taattgcaaa tgactttcat ttagattctt gtgtggatgt
      301 gatgagatgg caccgcagaa tgggtcagat gaacccgtca aacctgctcc atccggagct
      361 gcggcagcag ccgctgtgca aagtcctata gagttttctg caaccccagt ggcccaaaac
      421 tggtatgaaa atgccatctg catactccga aactccggat gcaagctgac cgaacaacga
      481 aggctgatcc ttcttctctt tgagcacggc cccggacatc tgacggcaca acaagtgttc
      541 gagtcggtaa cgcggtctca accggatata agccttgtca cagtctaccg caccctcgag
      601 cttcttaagg atgtgggagt tctgagcaag atcaacttca atgatggcgc cgaccgatat
      661 gaacggaacc ttggcaacgc cgggcaccat catcacctca tatgcaccgg atgcggcaga
      721 gtggttgagt tcggcgactg tgagatttcg tcgttagcgc gcaagctcga acaagccacc
      781 ggcttcacca tagacagcca ttggctcaaa ttgaccggac aatgcgccac ctgccggctg
      841 gcggctccag cttccacacc cggcacaaac aaggaggagt aaaagatgtc acatctacat
      901 attccagatg gggttcttcc actgccatgg atattggccg ggtttggggc aacagcaatc
      961 ctgcttcttt tcgccgtgtc ctccataagg cgccaagacc gggcacgcac cgttccccgg
     1021 atcgcgatac tctccgccgt catgttgctg gcgatgagcc ttcccatacc agttctccac
     1081 taccatgtta acttcacggt gctgactggc atgatggcag ggcccgctga gggattcata
     1141 gcaggattta tcacaaactt gattttggca ttgggtgccc atggcggaat cacagtaatc
     1201 ggactcaata ctctaataac gggcctggag atcaccctcg gctggctcct atggagcctg
     1261 tttggttccc gaaaaggccg tccatttgtg aaaaccgcca tcatcaccat tatcgtcttg
     1321 gccgtgtcca ccacagccat gctaggaata gttgccggca ccgagatcaa tccgtcgctt
     1381 gtgatgcacc accatcacac ccatacagtc catgaagaag aggagccatt cgatgaagcc
     1441 gaccatctcc ttccagatca cgattcccac gagcatggcc cagatagcga tacccgtctt
     1501 gcctctttca agacatttgc cacaatcgtc tacacagccg ggatagtagg ctgggccata
     1561 gaagcagtga ttacagggtc tgtcgtccag ttcatctcgg agtcccgtcc agaccttatc
     1621 ggcatggaca ccctcccagt acccggggac gacgatgacg aagaagaacc cagacacaca
     1681 ggggaggcct agacttggat atccgattca tcgacgagct ggcagcgaac ggtcgcactc
     1741 ctttgcacgc agcgctgccg tgggcgaagg ttcttgcatc ggccttactc ctcacatcaa
     1801 caatagcgtc atcatcgccc gccatggcag gcctcgtggc aggtgcggtc gttctgctag
     1861 catgcgctac aaagcagccg ctctggtaca cggcgcgcat tgctatttac ccggtagtct
     1921 ttgccgcact tttcgccata ggatcagtat ggtcgaatcc gttgtacgcc attaccatag
     1981 tgactaaggc tttcacagcg gggttgatag ccgcgactct catcaccacc acatcgtttg
     2041 tggatgtttt ctccattatt tcccatgtac tgccgagcct cgtggcagat gcgttattca
     2101 tgggctaccg ggctttcttc atcctcgtag atgaactgca aggactcatg gtcgccgtcc
     2161 gactgcgggg cggggtcagc ggaacccttc cacagcagct tcgtacatac ggccaaatgc
     2221 ttggagttct agtgatccat tcagccgaca tgactgagcg aatgtaccga gtaatgcagg
     2281 ttagaggcta ttcacagcgc atttccgcca caagcgagca tcggcctccc aatgcgatgg
     2341 actttgcctt gctcgcatat tccgcactta ctctagtggg ggtgttcctt tacagatgag
     2401 cgatgagatt attgccgaga taaagtgctt aagacacgtc tatcccgacc aaacagaggt
     2461 ggacctgtgc ggactcgact tttccgtccg caggggccag agagtcgcag ttctcggggc
     2521 aaacgggagc ggcaagtcaa ccctcatatt ccacctcctc ggcttgcttg cgccagtcga
     2581 cggcaaagtc agcgtcttcg ggctcagccc gtcaaagcag ttcaaggaga tgcgcaaccg
     2641 agtgggcgta gttctgcaaa acgtcgacga ccaaatcatc ggacctacag tatgggatga
     2701 tgtaacgttt accccgcggg cactggggtg gccgcgagat gagatagagg ccaagggcaa
     2761 ggctatcctc aacaggctgg ggatatggca tctgaggagg aaagtgccac actacctgag
     2821 cggaggcgaa aaaaggaagg ttgccctggc cggagcgcta atcaccgagc ctgagcttct
     2881 cataatggac gagcctttca ctgggcttga tccgcgttcc cgcacagagc tggcctcgct
     2941 cctcgcccag cttcatgcgg aaagcggaat aacatacatc gtctccaccc acgagatcga
     3001 ctacgttcca gcaatagcgg actgggtata tgtgctggca gggcaaggca agatggccct
     3061 ttccggcacg ccagaagagg tttttgccca gaccgacgcg ctaagggatg cagggatcga
     3121 gccccctgtc ctggcagatc tcttcgaatc cctgaaaaaa agagggtggg acgtttccgt
     3181 cccactcact gtagatcaag ccctcgagga gctggactat gccagagtgc gttgaccgca
     3241 cggcctgcag tgcggcaagc gccatttcct ggctgtcctg acttgagcaa gtcacaatgg
     3301 aggatcgcgc aggatactgc gaagcttgct caaccgatct taggaatctt gatcccgtga
     3361 ccggcggcga aagcctgggc cttttcataa cctgcatctg catgacgcat gaccccggtc
     3421 cccgggtctc cggtgagcac gcgttccagc ctgactgcgg cctccggggt tccatccgcc
     3481 accactacca ttccggcatg gagggagtag cctatgccga caccgccgcc atggtgaaat
     3541 gaaacccacg tggcaccgtt taccgcgtta agcagtgcat tgagcacagg ccagtctgca
     3601 atcgcatcag acccgtcttt catcccttcg gtctcgcgat tgggcgatgc aactgatccg
     3661 caatccagat ggtcgcggcc gatcacaata ggtgccttga tcttgccctg ggccacgaga
     3721 tcgttgatca tcaacccgaa tctggcgcgt tctccgtagc ccagccaaca aattcgcgag
     3781 ggcaagccct ggaacgccac cttgtcatgg gccatctgaa tccacttgac caggtgttca
     3841 tcgtaggcaa aatgctccat aatcacctcg tcagttacgt agatgtcctc cggatcgcct
     3901 gaaagcgcca cccacctgaa ggggcccttt ccctcgcaaa agaggggcct tacgtaggcg
     3961 ggaacgaagc cagggaaatc aaaggcatcc tttacgccag catcgaacgc gcgctggcga
     4021 atgttgtttc cgtagtcaaa aacgactgaa ccgcgatctt tcatgtcgag catggccctg
     4081 acctggatcg ccatggactc catcgcccgt ctcttgtact cctctggatt cgacttccga
     4141 agctcgccag cctcctcaag agtgaggccc gcaggtatgt atccgttaag aggatcatgg
     4201 gcagacgtct ggtctgttac gagatcaggt atgatcccgc gcctcacgaa ttcggggtgt
     4261 gtttctgcag cattgcccag aagggcgatg gagcgcggaa tccctttctc ctgggcttcc
     4321 cgggcaaggg cgatggcctc gtctatgctg tcggtcacca tctcgcagta gtcagtcttg
     4381 agtctgcgct ctatcctctc ccggtccact tcgaccgcaa tcacgactgc atcgttgaag
     4441 gtagcggcca acggttgcgc gccgcccatt cctccgagcc cggcagtgag aatcagcctg
     4501 cccttgaggc tcccaccaaa atgcttcttc gcagcggcgg caaatgtctc ataggtcccc
     4561 tgaagtatgc cctgggtgcc tatgtagatc cagcttccgg cggtcatctg tccatacatg
     4621 gtgaggccct tggcctccag ttcccagaag ttttcccatg tggcccagtg aggaaccaac
     4681 atggcgttgg aaatcaggac ccgcggagca tcggggtgag tcctgaatac gcctaccggt
     4741 ttgcccgact ggacaagaag cgtctcgtct ccttctaact ctttcaatga gtccactatg
     4801 gcatcgaagc actcccagtt gcgtgcggcc tttccagctc cgccgtatac gatcagatct
     4861 tcggggcgct cagccacttc cgggtcaagg ttgttcatga gcattcgcat ggcagcttcc
     4921 tgctgccacc ctttgcagga cagctcggtc ccacgcgggg ctcttattac tcttgccatt
     4981 acactatccc tccagccctg catatttgtg caaccttgtg ccttatgcct cgcacaagcc
     5041 tctcccaatc agtcactcca gttcgccgac tgccttttcc acctcgcgca acaaatcgcc
     5101 cgactctatc atctcccgca cccgcgcaat gtcgaccgcg agaattctat cggcgtcaag
     5161 atgtgcaacc ctttcccgca ccgcacgatg ggctgcccta gttcccgtgc caggctctaa
     5221 aggtgcccga aaatcaatgc cctgggccgc acagacgatc tctatggcca gtacattagc
     5281 gacgtttgtc gcaatctgcc tagccttgcg cgccgcgatc gtccccatgc tcacatggtc
     5341 ctcttggttg cctgacgttg gaatggagtc gaccacagcc ggagacgcca atatcttatt
     5401 ctccgagaca agggccgcgg ccgtatactg ggctatcatc atcccagaat tgagtccgcc
     5461 atgttcagtg aggaacggag gcaattccga aagctgcggg ttgaccatcc gctcagtgcg
     5521 ccgctccgag atgcttgcaa gctcagatgc ggctattccc aagaagtcca tagccatggc
     5581 catcggctct ccatggaaat tgcccccgga tatcacgtcg ccgtcatcgg cgaagatgat
     5641 gggattgtct gtcaccgagt tcatctctgt ttccacagcg cgaagggcat agtctatagc
     5701 atcgcgagcc gctccgtgaa cctggggtgc gcatctcaat gaatatgcgt cctgaacctt
     5761 ggggcaatcc atgtgtgatg ccctgatacc gcttccttcc atgagacgca gcaggttcct
     5821 ggctgatgca gcctgcccgg ggtgggggcg aactgcatga actctgctat catacgccgc
     5881 tgtagtgccg cggagagcct cggcgctcat ggcggccgca atgtcggcaa ccttggcaag
     5941 ccgagcaacg tcccacacag caagagcgcc tatggacgtc atcacttgcg tcccgttgat
     6001 gagagcaagc ccttccttgg ccctcaaagc cacaatgggt atccgggctt tcgacatcgc
     6061 actggcccca tccataactt gcccttcgta ttctgcctcg ccctcgccga tcatgaccaa
     6121 catggtatgg gcaagagggg ccaagtcgcc actggacccc acagatccct gtgacgggac
     6181 tacgggatga atcccagcgt tcaacatgga aagcagagtc tccacaactt caactctgac
     6241 tcccgaatat ccgcatgcga gcgcattagc cctgagcagc aaaatccccc tgacaacttc
     6301 ccggggcagc gtctcaccta ctccgcttgc atgcgaaaga aggaggttgc gctgcaattc
     6361 ctcggcgtct tggggggata taaccacatc tgcaaacttt ccaaaaccag tcgtcacgcc
     6421 atatacaggc ctgtcacgct cgaggagcga ttctacgaaa ccgctcgcct cagcaatcct
     6481 aaagcgggct tcagaggaga gtgatacggt agccccctca cgcgcaacag ccacaacctc
     6541 ccgcactgtc aggtctttcc ctttaacggc tacatgcaca atggtccctt cctttcgcca
     6601 ataccttaca gctgaaacat cacaacctca gaacggcggc aaaccacagg gcaattatcc
     6661 acagttgggc agcggattta caacttataa aaaagcccaa gctgcgatcg ggcaggattg
     6721 atgtgaaccg caataaccat accatataaa ttttgtcgtc gagcacgcgt tttcctgcca
     6781 gggcgccccg gctaagaaga actattaatg cttcactcct gcacccgcat tcccgattgg
     6841 gcattggtat cgcgagcccg caagcgctcc gttatctccg gaaaacaatg tcatgcatga
     6901 cagggcctgt ttcggcccag atgctgtagg gagcggagat tttcagagcc cggtacatga
     6961 agacacatct cgcctttccc atttgcggcg gcaagcgggc caatcccacc tacgccctaa
     7021 tttcaaccaa gcacaagtgt acatacgaag gcgatgcaga tggccgcaac aatggtcatt
     7081 gggattccta ctacagccat gtcgcgtacg ctgaagcact ccgagtcgtg gaataccagg
     7141 ttcgccgggg cagacgtcac cagtatgaac gcaagcggcg aagccagcgc cgccggggca
     7201 agaaacgtcc agggatcaac ggctgctccg ttgatggctg cgagaactac cggcatcact
     7261 atcgaagccg ttgcagtgtt gctcgagaac atgagtttga agagcattat cgcagtggaa
     7321 agcaggagcg ccctgggaaa cggctgcaaa gctgccattc tcccaaacac tgcgtttgca
     7381 agccatgccg cagcccctga atcatatact ccggctccca atgacatgcc cacagccacg
     7441 agaaccagat tgccccagtt gatcttgtgt tcaaggacat cccatgaact agttccgttg
     7501 acgggcagaa gatatacgac agcgcctatg atcgcgccca gcgacatagg catgtccatg
     7561 ccgaatgcct tcttcagcca atcactgaga aaccagagcg tgagcatgac tcccaatccg
     7621 acgagagccc aaatctcatg ggccgtcaac gggctgccgt ttgccacgat agcgcctctg
     7681 ctcttgccct cgctcccgca ttcaccgtca tcagcatccg cgctgtctac acgcagctcc
     7741 tcgatttcgg gagggaaggc catgattaat atggcccatg ccggcgccat catgagcatg
     7801 gctgcaggga accccacagc catccattga gtgaaaccaa gatcaacgcc ggcaaggttt
     7861 cgcagaaacc cgatggcaat cagatttgaa gcacctcctg caggcgtggc gatgctccct
     7921 atgagggcac cccatgcaca ccctatggag agggccgtgc cgaaattaga atgtccggga
     7981 acgaggccat tcgagactat tatggcaaca gctatcggct gaagtatcgc ggcaactgca
     8041 aggtttccca gccacgccgc acagaagccg ctaaaaagca gaaaagccag gactaccctt
     8101 ttcggatcgc tcccagccat tcttacaatt ctcttcgaca cgcgcaaggc aagtccggac
     8161 tcgactacac ctgttgaaag tatcatgatg ccgagcagaa agggcacgat atccgaaccg
     8221 gctccggact tgatgacgtc tgtccacccc atcacgccga ataaaggaag catcacggcg
     8281 cccaggaggg cggtcactgg aaaggggaaa acctcgaggg cccacaggat cgcagtaaac
     8341 atcacaatcc ccagcgccga ccttcccgtt gcagtaagac tcaggatctc tcccgaagcg
     8401 gccaccacgt ctggcaatgg cacgaatctg gcaaccgcaa aggcaccggc tgcaatagcc
     8461 gttccccagt agaccctggg caatctacca ctatctggtt ctgacatcta ttacatcctc
     8521 tcagctgcat gttctctttt cacatccggt tttgcccacg gttttatgct cgccagagac
     8581 ggcattactc gtagatcgca gcgcgacctt ccgaatggct gcctcgcgca taatactgcc
     8641 ctgacgctgc accgcgccag ggcagttaaa tgttgcggac cacaaagccg cctcttttta
     8701 cgcgggggca ccctcaggcc ccggatcgcg ctgcttgccg gccctgagcc gttcttcgat
     8761 tgcagcgatt ccggatttgg cttcctcagg ctgggaaccg ccgactaccg ccgccgagcc
     8821 gagcccggca tcggacttgt cagccccata gtccgaatcc tgagcctcat tgtcatcgct
     8881 caggagcgca tcgagttctt cgccttctat agtctccctc tccttcagcg cagtcgcgag
     8941 cagatggagt ttatccatgt tctgggtcaa catctcagta gccttgttgt aagcttcagt
     9001 taccatgcga tgaacttccg cgtcgattct tgacgccact tcttcgctgt agttccggtc
     9061 gcgggcgatg tctcgtccga ggaatatctg ttcttcatga cttcgcccgt aggttatggg
     9121 gcctagatcg ctcatgccgt actccatcac catcttgcgc gcagtcttgc tagcgcgctc
     9181 caaatcgttt tgggccccag tgcttatctc gcccaacacg atctcttcag cgactctgcc
     9241 gcccaatgca acaacgatct cgttgaacat ctcgttcttg gtggcgtaac
//
LOCUS       DAOSAI010000048         9160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4955_length_9160_cov_3.329379, whole genome shotgun sequence.
ACCESSION   DAOSAI010000048 DAOSAI010000000
VERSION     DAOSAI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4955_length_9160_cov_3.329379"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="PLM83_02995"
     CDS             complement(<1..1187)
                     /locus_tag="PLM83_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70409.1"
                     /translation="MEPVSILHTSDLHLDHVLTEYRRDVRAVRRRELVDTFNFIVSEA
                     IRLNVRLMLIPGDLICADSVADSVVEEVRNGFARLGRSSIFVAVTPGEAEENGGLELL
                     REACSGENVHLFMAEEWSSVSPFPGVTVWGLRTTSRNSTMPVLSNLVVEGSGMHVGLM
                     HGTFAGTPGLDSSICPISPDALSQSGLDYLALGHYHNMVNCSVGKATCWYSGSPVHLN
                     FDTRGERHALLVSLREDGTRVNPMKVPSRQHRVITVDIAGKSGAEIRERLSDMANAEL
                     CLRVELEGRVDPALRPILAGLAREFEDRFFHLEVADRTELVVRGPRGEDRRRPGRGST
                     RKEVRLPRTDQPAGKNYHANCTGMDALERDLLEAEQTFTAKMIALLTQSEEANAAAPG
                     SFAG"
     gene            complement(1541..2659)
                     /locus_tag="PLM83_03000"
     CDS             complement(1541..2659)
                     /locus_tag="PLM83_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOK70410.1"
                     /translation="MWEAGAQEVLRDEMFWRIIRVINLLLASGKGMSVAELAAACEAT
                     PEVIRDDIQRLSEYMRAPLMSNLDQGCDACDDDDPLCPDQEVWSLASKDFAFPITSLS
                     VKEARTLLAILKGSEPSCGAATSACGGDAKRDGMGGAERAVSKLEASLIKAGSSDEVA
                     LAASRRVVKAGRTMYGEGQEESKLDALGLFVARREPIHISYVNASGQRSERDTCPVAL
                     VYDWRTCAWYLYGYNERHGGYRHYRVSRILGYGPSSVEIDAPSDEEVERHVKTCWGVE
                     CSCEQVEVVVRFLNDFNVIERMYADTADRPDAVYEVQTDGSVIYRDMMPGWNEFRTWV
                     ATFGESAEILAPPELRQSMAEAVDRVLDRYEKVGMLLG"
     gene            complement(2622..3956)
                     /locus_tag="PLM83_03005"
     CDS             complement(2622..3956)
                     /locus_tag="PLM83_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOK70411.1"
                     /translation="MAGGRDTRLLSILEMVKSSGGSCQVDTILEAFDISMRTLQRDLA
                     KLRMVGAKIEIRDALVVCEDDGGGYHARSSEAAMRQLAILTYLTGGPSTITSIVTYLN
                     DPIRDFSIDERTVRRDVDYLESRGLVEAPGQWSEPGGKTGPSCRGECVDRSGAYRLSS
                     AFMPRFSLTCDELVAVVLALESAPEAVADHATVVSIRDKLLASVAPQTGPAHYVTRRR
                     FTKGRTRRSDPAILRKVDTLELASLEGRVVRIRYRGTGDGASKVRDVEPLGVVYYWFH
                     DAWYLVARCRRADELRHFRVDRIDGMMLTSDRYEYPEDFDLSDYTSEMWGVYAGEPTS
                     VKVLFFDEMNVVDRLHAEVLGRPSARLCQVDDGVWELVDTVMGLSEFRTWLRTFGSSA
                     VVIEPENLRNQLIASAERMKEIYSGPSVMAGAGEAKEDAIHVGGRRTGSPEG"
     gene            complement(3985..4623)
                     /locus_tag="PLM83_03010"
     CDS             complement(3985..4623)
                     /locus_tag="PLM83_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK70412.1"
                     /translation="MQNVALLPELQLIAAVQEENCGEAFAELLDRYSRAIYVWTKPFF
                     NEDVVSRDDLLQEARLGFLAAVRAYNPKLASFFTFAELCVNRRLCSVARSFRRSKNTP
                     PAPVLPLWGSARSWDSDDRNLIESVADTDAVDPVEEAIAHDSAARIMNVASSALSTLE
                     LEVFKGLAEGASYRELAVNMGVETKTVDNAAFRARRKLRELMFQEIERDVTA"
     gene            complement(4737..6245)
                     /gene="scfB"
                     /locus_tag="PLM83_03015"
     CDS             complement(4737..6245)
                     /gene="scfB"
                     /locus_tag="PLM83_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HOK70413.1"
                     /translation="MPDLLPVCLQDIVYAWEPGLSEIADILDIERRRPSAAGIRRNWH
                     WFQAADARFLLDVQSGSLHAIDDAAWDVLEALVSSCDQAGTRECAAADLAAAAGVDVS
                     QTEEILHDLAVLIDEGTLAWPDDEDPVPSALGPGVVKAMCLHVAHDCDMRCGYCFAGT
                     GGFGGRRSLMPPEIACAALDLLFDISGHVKHLDVDFFGGEPLLAMDTVVRAVEHGRKL
                     EAETGKVLKFTITTNATRLTPEIGKFLNDNQIAAVLSLDGRPQVHDAVRKGASGESTF
                     DRCSANALSFVKTRPEGPDGEYYARGTYTRKNTDFVEDVLFLADMGFASISFEPVVSP
                     PSSPYSIRESDLDEIYRSYDRLVREMDARSEEGRGFRFFHFDLDVAKGPCLAKRLLGC
                     GAGREYVAVTPDGELYPCHQFVGREGFVMGDVFHGIEKPEIGERFGAAHIYAKEACRD
                     CWARYLCSGGCHANAHQMNGTLMEPYAIGCMITRKRLESALAFAGLESLRCA"
     gene            complement(6176..6337)
                     /gene="scfA"
                     /locus_tag="PLM83_03020"
     CDS             complement(6176..6337)
                     /gene="scfA"
                     /locus_tag="PLM83_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOK70414.1"
                     /translation="MNTDSKKNTKHVKTIIASDLKSTVSSGGCRECQTSCQSACKTSC
                     TLGNQACRR"
     gene            complement(6368..7396)
                     /locus_tag="PLM83_03025"
     CDS             complement(6368..7396)
                     /locus_tag="PLM83_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOK70415.1"
                     /translation="MKDLVKRLTEAYGPSGSEDKVREIIKAEVEKFADEIRVDSLGNL
                     ICRVAPKSPADGSDPKRIMVAAHMDEIGVLVSYIDDKGFLRFSPVGGLSPYVLLGQKV
                     IFENGTVGVFGTEKLEDMKSLAFDKMFIDIGASSKDEAEKKVRVGDSAVILREFSDLG
                     DRVTSKTMDNRISCAVVIEAIKSVRPERHEAYFVFTVQEELGLRGAKAAAYGINPDVG
                     IAVDVTTWGDTPECAPFNVVLGKGVAIKAKDSSVIAHPKVKNLMIDVAEKKGILYQME
                     VLERGGTDAGAIHLTREGVPSGTISIPCRYVHTPCEMVDMRDVDAATRLLVGVVEELA
                     SSSGGGIL"
     gene            complement(7368..8420)
                     /locus_tag="PLM83_03030"
     CDS             complement(7368..8420)
                     /locus_tag="PLM83_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOK70416.1"
                     /translation="MLLAKLSEASGISGQEGEIRATIRNEIAPYVDEIKIDPIGNLVA
                     LKRGRTGKARVMLAAHMDEVGLMVVGFESNGLLHVDSVGGVDPRVLVSKMVYVGRNKL
                     PGVIGSKPIHLLEREDMDRATKMDALLVDIGAKNKEDAEKLVKLGDPVVFATKCEPLG
                     DGFMKGKAFDDRVGCCAIIEALKVQHDFDLYGAFTVQEEVGLRGAGVAAFQIEPDLAI
                     VLEGTTAHDVPETPEHSVSTVPGKGPAIVYMDRSWVGDKRIIDRLVETAEAASVPYQF
                     KRTVTGGTDAGRIGLTREGIPAAVVSMPCRFIHSPVSVINLSDFENYVKLAGLFLDSL
                     DERGLPIDEGSCKKTN"
     gene            complement(8411..>9160)
                     /locus_tag="PLM83_03035"
     CDS             complement(8411..>9160)
                     /locus_tag="PLM83_03035"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOK70417.1"
                     /translation="VHGRRDISGVIGAIPPHLQDPKDKDKSWKWEELVVDCGMSAERL
                     RELVRVGDVVSFDRTGVELANDRISGKSMDDRAGVAVMYECAVELQKLKHDCDVYFVA
                     TVQEEVGYGGSITSTYGITPDIGIAIDVGHGDMPGLPEHHTLKLGGGPAISYGPHVHP
                     HIFSKLKEIAEERGIAYQVEAGTSPRGTDAYAMQMTRSGVATGLISVPLRYMHTSVET
                     LSVEDVKKSGQLLAFFASAVDSRFREGLTCF"
BASE COUNT         2104 a         2921 c         2107 g         2028 t
ORIGIN      
        1 cccgcaaaag acccgggagc tgccgcatta gcctcctcgg actgcgtcag taatgctatc
       61 atcttggctg tgaaagtttg ttccgcttcg agtagatccc tctcaagcgc gtccatcccg
      121 gtgcaattgg catgatagtt cttccctgca ggttgatctg tcctgggcag acgcacttcc
      181 ttccgtgtac tgccacggcc cggtctcctc ctatcttcgc ccctaggccc cctcaccacc
      241 aattctgtgc ggtcggcaac ttcgagatgg aaaaagcgat cctcaaattc tctcgccagc
      301 cctgccaaga tgggtctgag agccggatcc acgcgtccct ccagttcaac ccttaagcac
      361 agctcagcat tggccatgtc actcaggcgt tctcgaattt ccgctcccga ctttcccgca
      421 atgtcgacag taatgactcg atgctgccga ctaggaacct tcatggggtt tacgcgagtc
      481 ccatcttcac gcagactcac gagaagtgcg tgccgttcac ctctcgtatc aaagttgagg
      541 tgtaccggtg accccgaata ccagcacgtc gcctttccca ccgagcaatt gaccatgtta
      601 tggtaatggc ctaaggccaa gtaatcgagg ccagactgtg aaagcgcatc gggcgaaata
      661 gggcatatcg aagaatcaag cccaggagtc ccagcgaaag taccatgcat caaccctaca
      721 tgcatccccg agccctccac aaccaggttg gaaagaacgg gcatagtgga attccgagaa
      781 gtagtcctca gtccccatac agtgaccccc ggaaaaggcg aaacggatga ccattcctct
      841 gccatgaaaa gatgaacatt ctccccagag caagcctccc gaagcagctc caaacctcca
      901 ttttcttctg cttctcccgg ggtaaccgcg acaaaaatcg aactcctgcc aagccttgca
      961 aacccattac ggacctcttc aactaccgag tcggccactg aatccgcaca gatcaaatcc
     1021 cccggaatca gcatcagtct cacattgagc cggattgctt cactcacaat gaaattgaat
     1081 gtatccacca gttcccgacg gcgtacagcg cgaacatctc gtctgtactc ggtgagcacg
     1141 tggtcgaggt gaaggtcgga cgtgtgcaga atgctcacag gttccaaaac cctgccctcc
     1201 cgcagttgta gaatgtgatg aaccgcaaca cagaagatcg taatacgccg caattcacga
     1261 aatagcgacg ttaatgttgg ttatgcattg ccattaccat atgtataagc ttagctttca
     1321 aatgctaact cgcactgtct atttcttcgt tacgccccct ttcccttcat aactcttccg
     1381 gcataaagcg aggcgcgccc cgaagggcgc gcctgttaat gcttgcagtg ccgacaatat
     1441 tcttcattca gatgccgaat cacgctcggc gcgaccatgg aaagcgccaa cagcccgatt
     1501 ctccccgccc gctcccaaga tcatgtctgt ccacgtctct ctatcccaac agcataccga
     1561 ctttttcata cctatccaac accctatcca cagcctccgc catactctgc ctcaactccg
     1621 gaggcgcgag aatctcggct gattccccga acgtggccac ccaggtgcga aactcgttcc
     1681 agccgggcat catgtctcga tagatcaccg acccatccgt ctgaacctca tacacagcat
     1741 ccggccgatc agccgtgtcg gcatacatcc tctcgatgac attgaagtcg ttgaggaagc
     1801 gaaccacaac ttccacctgt tcgcagctgc attccactcc ccaacacgtc ttcacgtgtc
     1861 tttcgacttc ctcatcggat ggcgcatcta tctcgacact tgacggacca tatccgagga
     1921 ttcgcgatac cctgtagtgt cgatatcctc cgtgcctctc attgtatccg tacagatacc
     1981 acgcgcacgt cctccaatcg tagacgaggg cgacaggaca ggtgtcacgc tcacttctct
     2041 gccctgacgc attcacatac gagatgtgta tgggttccct cctcgccacg aagaggccca
     2101 aggcatcaag cttcgattcc tcctgtccct cgccatacat ggtccgtcct gccttgacca
     2161 cccgcctgct cgcagcaaga gcgacttcat cagatgaacc ggccttgata aggctcgcct
     2221 ctagctttga cactgcccgc tcagcgccac ccattccatc gcgtttcgca tcaccgccac
     2281 aagcggacgt agcagccccg catgaaggct cagacccctt aagtatggcc aagagagttc
     2341 ttgcttcctt cactgaaaga gacgtaattg gaaaagcgaa atccttagag gcaaggctcc
     2401 atacctcctg atcgggacag agaggatcgt cgtcatcaca ggcatcgcac ccttgatcca
     2461 agttgctcat caatggggca cgcatgtatt ctgacaggcg ttggatatca tcgcgaatta
     2521 cctcgggcgt agcctcgcat gcagctgcaa gttctgccac gctcatgccc ttgcccgagg
     2581 ccaacagcag attaatcact cgtattatgc gccagaacat ctcatccctc aggacttcct
     2641 gtgcgcctgc ctcccacatg gatagcgtcc tccttcgcct ccccagcccc tgccatgacc
     2701 gaagggccag aatagatctc cttcatcctt tcagcactgg caataagctg atttcttaag
     2761 ttttcaggct cgataaccac cgcagaactc ccgaatgttc gcagccacgt tctgaactcg
     2821 gaaagcccca tgaccgtgtc aacaagttcc cataccccat cgtccacctg gcatagtcga
     2881 gcggaaggcc ttcccaacac ctctgcatga agcctatcca ccacattcat ctcatcaaag
     2941 aacaaaacct tcactgaagt aggttcgcca gcgtagactc cccacatctc ggaggtgtaa
     3001 tccgataaat caaaatcctc tggatactcg tatctgtcgg aagtcagcat catcccgtca
     3061 atccgatcca ccctgaagtg ccttagctca tccgcccttc ggcagcgagc aacgagatac
     3121 catgcatcat gaaaccagta gtagactacc cccaacggct ctacgtctct gaccttgctc
     3181 gccccatcgc ccgtgccccg atacctgatc ctcacgaccc ggccctccag cgacgccaac
     3241 tccagggtat caaccttcct aagtatggcc ggatccgacc ttcgagtccg gcccttggta
     3301 aaccttcgtc tagttacata atgagcaggc cccgtctgcg gcgccacact cgcaaggagt
     3361 ttgtcgcgga tggaaacaac agtagcgtgg tcagccaccg cctccggcgc agactccagc
     3421 gcaagcacca ccgccaccaa ctcatcgcag gtcagcgaaa atcttggcat gaatgcactg
     3481 ctcagcctgt acgcgcctga tctatcgaca cattcgccgc ggcaggatgg cccggttttc
     3541 ccaccgggct cgctccactg gccgggagcc tccacaaggc cgcgagactc caggtaatcc
     3601 acgtcgcgcc tcacagtccg ttcgtcgatg ctaaagtcgc gtatggggtc attcagatac
     3661 gttactatgc ttgttatggt tgaggggcca cctgtaaggt atgtgaggat ggccagctgt
     3721 cgcatggcag cttctgagga cctagcgtgg tatccgccgc catcgtcttc gcagacgact
     3781 agggcatcac gtatttcaat ctttgcccca accatcctca gtttcgccaa atcgcgttgg
     3841 agagttcgca tggatatgtc gaaagcttcg agaatggtgt caacctggca tgacccccca
     3901 gacgacttca ccatctcgag tatcgataat agcctggtat ctcttcctcc agccatatgc
     3961 aatcacccag aggacaattc gacatcaggc cgtcacgtcc ctctctatct cctggaacat
     4021 aagctcccgc aacttcctcc ttgcccggaa tgctgcattg tcgactgtct tcgtttccac
     4081 acccatgttt acggcaagtt ctctgtagga cgccccttca gccagcccct tgaagacttc
     4141 aagctccaac gttgagagcg cactagacgc cacattcatg attcttgctg cagaatcatg
     4201 cgcaattgct tcttcgactg gatcgacagc atcagtatcg gctacagatt caatgaggtt
     4261 ccggtcatct gaatcccacg atctagcaga accccatagc ggaagaaccg gcgccggagg
     4321 ggtgttcttc gaacgccgga atgaccttgc aacgctgcac agtcttcgat tcacacacaa
     4381 ctccgcaaat gtgaagaatg atgcaagctt aggattgtat gcgcgcaccg ccgctaggaa
     4441 tccaagccgg gcttcctgta acagatcatc cctgcttacc acatcctcgt tgaagaaagg
     4501 cttagtccat acgtagattg ccctggaata cctgtcgagc aactcggcaa aagcctcgcc
     4561 acaattctcc tcctgaacgg ctgcaatcaa ctgaagctcg ggcaacaaag caacgttctg
     4621 cattgccata cctcccagaa tgtcgaagtc ctcggctaga caccgaggac atggtaacat
     4681 tccggtccgg cacaaacctg tcacatcagc aaaaccttgc ctggcggcgc catcactcag
     4741 gcgcagcgca acgattcgag gcctgcaaaa gcgagagcgc tctccaggcg tttgcgcgtt
     4801 atcatacatc ctatggcgta tggctccatt aaggttccgt tcatttggtg cgcgttagca
     4861 tggcacccgc cggaacacaa ataccgcgcc cagcaatcgc ggcacgcctc cttagcatat
     4921 atatgcgcag cgccgaacct ctcgcctatc tcaggctttt caatcccgtg gaacacatct
     4981 cccatcacga agccctccct gcccacaaac tgatggcatg gatagagctc cccgtctggg
     5041 gtcactgcca cgtattcccg cccggccccg cacccaagca accgtttggc aaggcacgga
     5101 cccttagcca cgtcaaggtc gaaatggaaa aaacgaaatc cccgtccctc ctcacttctc
     5161 gcgtccatct cgcgaacgag tctatcgtat gacctataga tctcgtcgag atcggattcg
     5221 cgaatcgaat aaggagatga tggcggcgaa acgacaggct cgaaagatat acttgcaaaa
     5281 cccatgtccg cgaggaaaag cacgtcttcc acgaaatccg tgttctttct cgtatacgtg
     5341 ccccgcgcgt agtactcgcc atcagggccc tcagggcgag tcttaacgaa actgagcgca
     5401 tttgcagaac agcgatcaaa tgtagattcg ccggaggcgc ccttgcggac tgcatcatgg
     5461 acctggggcc ttccatcgag gctgagcacc gccgcaatct gattgtcatt gagaaacttc
     5521 ccaatttcag gcgtgaggcg agttgcgttc gtcgtgatgg taaacttcaa tacttttcct
     5581 gtctccgcct ctagtttccg gccatgctct accgctcgaa ccaccgtatc catggccaga
     5641 agcggctctc cgccgaaaaa gtcgacatca aggtgcttca cgtggccgct aatgtcgaag
     5701 agaagatcga gcgcagcgca tgcgatctca ggcggcatca ggcttcgcct tcctccgaac
     5761 cctcccgtac cagcaaagca gtatccgcac ctcatgtcgc aatcatgcgc cacatggagg
     5821 cacatggctt tgaccacccc ggggccaagt gccgacggga ctggatcctc atcgtcaggc
     5881 catgcaaggg ttccctcatc aatcagcaca gctaggtcgt gcagaatctc ctctgtttga
     5941 ctgacgtcaa cgcctgccgc ggcagcaaga tctgccgcag cgcactcccg agttccagct
     6001 tgatcacagg aggatacgag cgcctccaaa acgtcccatg ccgcatcatc aatggcatgc
     6061 aagctgccag actggacgtc aagaaggaaa cgagcgtccg ctgcctggaa ccagtgccaa
     6121 ttgcgcctga ttccagctgc cgacggacgc cttcgctcaa tatccagtat gtcggctatc
     6181 tccgacaagc ctggttccca agcgtacacg atgtcttgca agcagactgg caggaggtct
     6241 ggcactccct gcaaccgcct gacgacaccg tagacttgag atcagatgct atgatcgtct
     6301 ttacatgctt cgtattcttc ttgctgtcag tattcacgct tattcccccg tttgaccgat
     6361 tacacgtcta gagtatacca ccgccagaag aggatgccaa ctcttccacc acacccacca
     6421 gcagccgggt tgcagcgtca acgtccctca tgtccaccat ctcgcaggga gtgtgaacat
     6481 atctgcacgg aatagagatc gtccccgaag gcacgccttc acgcgtcagg tgaatagcgc
     6541 ccgcatccgt ccctccgcgt tccaggactt ccatctggta tagaatcccc ttcttctcgg
     6601 caacatctat catgaggttt ttgacctttg gatgagcgat tacacttgaa tccttcgcct
     6661 tgatagccac gccttttcca agcacgacgt taaaaggcgc gcattcagga gtatcacccc
     6721 aggttgtaac atccaccgct atcccaacat cgggattgat gccatatgcc gctgccttgg
     6781 caccacgcag acccagctct tcctggacag tgaaaacaaa ataggcctcg tgacgctctg
     6841 ggcggacgga ttttatagcc tctatcacca ctgcacagga gatccggttg tccatagtct
     6901 tcgaggtcac tctgtccccc aaatccgaga attcccgtag tatgacggct gagtccccca
     6961 ccctgacttt cttctcggct tcatccttgc ttgatgcacc gatatctatg aacatcttgt
     7021 cgaacgccaa agacttcatg tcctccagct tctcggtgcc gaacacgccc acagttccgt
     7081 tttcgaagat gaccttctgc cctaagagta cgtatgggct gagcccgccg actggagaga
     7141 accgcagaaa tcccttgtca tctatgtatg aaacgagcac tccgatctca tccatgtgag
     7201 cggcgaccat gatccgtttg ggatcgctgc cgtctgcagg cgacttcggg gccacgcggc
     7261 agatgaggtt gccgagggag tcgactcgga tttcgtcggc gaacttttcg acctcagcct
     7321 tgataatctc cctaaccttg tcttcagacc ctgacgggcc gtatgcttca gttagtcttt
     7381 ttacaagatc cttcatctat cggcaaaccc ctttcgtcaa ggctgtcaag gaacagcccg
     7441 gcaagcttta cgtagttttc gaaatcagag aggtttatca ccgaaaccgg cgagtggata
     7501 aaccgacacg gcatcgagac tactgcagca ggaattcctt cccgtgtcag gccgatgcgt
     7561 ccggcatcgg tgcctcccgt cacagtacgc ttgaactgat acggaacact tgctgcctca
     7621 gctgtctcga ccagccggtc gattatgcgc ttgtcgccca cccatgagcg gtccatgtat
     7681 acgatggccg gacctttacc cgggaccgtg gagacactgt gctccggggt ctcaggcaca
     7741 tcatgggccg tggtgccctc caacacgata gcaagatcgg gttcgatctg gaaggcggca
     7801 actcctgccc ctctgaggcc tacctcttcc tggactgtaa aagccccgta aaggtcgaag
     7861 tcgtgctgga ctttgagagc ttctataatc gcacagcaac cgacgcggtc atcaaatgcc
     7921 ttgcctttca tgaacccatc gcccaatggc tcacacttag tcgcaaaaac cacagggtca
     7981 cctaatttca cgagtttctc ggcatcttcc ttgttttttg cgccaatatc cacgagaaga
     8041 gcgtccatct tagtggcgcg atccatgtcc tcccgttcca atagatggat cggcttggat
     8101 ccgataacgc cgggcagttt gttgcgcccg acatacacca tctttgagac gagaactcgc
     8161 ggatccacgc ctcccacaga gtccacgtga agaaggccat tggattcaaa acccaccacc
     8221 atcaagccga cttcgtccat gtgcgcagca agcattactc tggcctttcc cgtcctgcca
     8281 cgcttcaaag cgacaaggtt gccaatcgga tcaatcttga tctcgtcaac atagggagcg
     8341 atttcgttcc ggatagtggc gcgaatctcg ccctcctgac cggaaatgcc cgaagcctct
     8401 gaaagcttgg ctagaagcaa gtcaatccct ccctgaaccg ggagtcaact gcgctcgcga
     8461 agaatgccag caactgcccg gattttttca catcttcaac cgagagagtt tcaacagatg
     8521 tatgcatgta gcgaagaggc acggaaatca gaccggtggc cacgccagaa cgtgtcatct
     8581 gcatggcata ggcatcggtc ccccgcggag aagtgcccgc ttccacctga tacgctattc
     8641 cgcgctcctc tgcaatctcc ttcaacttgg agaagatgtg gggatgcaca tgcgggccat
     8701 agcttatggc aggccctccc cccaacttca gcgtatgatg ctccggcaac cctggcatgt
     8761 ctccatgacc aacatctatg gcgataccta tatcgggcgt tatcccatag gtgctcgtga
     8821 tgctgccccc gtaaccaact tcctcctgaa ctgtggcgac gaagtaaacg tcacaatcgt
     8881 gcttgagctt ttgcaattca accgcgcatt cgtacatcac cgcaacaccg gcccggtcat
     8941 ccatgctctt accggaaatg cggtcgtttg ccaattccac acctgttcga tcgaagctga
     9001 caacgtcgcc cacgcgcaca agctcgcgca gcctctctgc ggacattccg cagtccacaa
     9061 cgagctcctc ccatttccac gacttgtcct tgtctttagg atcctggaga tgcggcggga
     9121 tcgcacctat gaccccggaa atatcgcgtc ttccatgcac
//
LOCUS       DAOSAI010000049         9154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_4958_length_9154_cov_4.548522, whole genome shotgun sequence.
ACCESSION   DAOSAI010000049 DAOSAI010000000
VERSION     DAOSAI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4958_length_9154_cov_4.548522"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..1005)
                     /locus_tag="PLM83_03040"
     CDS             complement(292..1005)
                     /locus_tag="PLM83_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HOK70418.1"
                     /translation="MEIDKSANLPVREMLEGFCTSYIAKTPNPVDISFGFLINDGQWW
                     TVSIQKEGSYTIANCKPSKPTFYLVSTAKTLEDIYLGKMHFLTAAGRAKMNDYAPLDV
                     RFIDGYSPPKDLDLMDFAFHFFVIGEPEKILFGKDHARVVHGGYAVPLVYTSGLRTAW
                     YRVEQGMVINEKEDDQYNPFNTLVIGTKGEGSIKLGHTEYRFAEGEAYIIPANTAHSF
                     WTDDEDGLEFVIIMYGEGA"
     gene            complement(1045..2175)
                     /locus_tag="PLM83_03045"
     CDS             complement(1045..2175)
                     /locus_tag="PLM83_03045"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK70419.1"
                     /translation="MDLISGLQQMGLTEYEAKIYAALVKQPSITGYEASKLSGVPRAK
                     VYETLESLVRKKAAYSLSVDGKQYYTPISHHSLIARFQDELLDLTTYLSVELDRLSKG
                     RTEDPFSVVTGYDLVTETAERMCARSQRRLYISVFAEDLPRLAGEIAKAKSRGVKVFT
                     LVYGEGKGIVEDAVEHYVSPLQYLQAAVYGRWMGLVADQDEGLMAQIAGPDDTYGLSS
                     TLPGIIMPITLWIQHDIAAYVVAERLNLPPTVTDGLEDLWSLAPEEGPQAPEHGNKSL
                     DDLFEVAKRKLEHNAGTLKDGIIEICFTDDGITYHVSKGQHKVEIEQGAKEEADLRLA
                     LSKADFEGLMTGFLSPEAFMTRGRIEIDGDLGMVAYLNAMFR"
     gene            2698..3213
                     /locus_tag="PLM83_03050"
     CDS             2698..3213
                     /locus_tag="PLM83_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70420.1"
                     /translation="MASAYREIRPPAKRFERSCVSVLLWFVGRGFQAAAHVDKHVQDE
                     VCGWKDGTIIAIGVAPCGPHMSVRKTGNRLRFLGNVKAPDADAVIEFKSLDAALLVLT
                     GQIGIDRAYAEHRFSLKGDLFSIGMPLVRCLYIVESYLFPKSIAKKILKRWPKRSSSG
                     FAVYFHTIFGV"
     gene            3231..4412
                     /locus_tag="PLM83_03055"
     CDS             3231..4412
                     /locus_tag="PLM83_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOK70421.1"
                     /translation="MIPSYYEFINSVKILSGKNALENIPAELRGFEARKPILLTDKML
                     VKIGLAQQVVDACGGSEIVIGAMYDDIPPDSSVEVVNDIVRVYREKGCDSVIAVGGGS
                     VIDTAKGVCICLTEGTDDLLDHMGVDILRRRKTPFVVVPTTAGTGSEVTGVAVIANPA
                     KNVKMEFVSANILPDVAVLDVRMTKSLPPRLTASTGMDALVHAIEAYTCLQRNPLSDA
                     HAHAAINLIRKYLPRAVRDGKDEEARLAMANASLLAGVAFSNSMVGIVHAVGHACGGV
                     LRAPHGDCMAILLPYGMEYNLSAVSERYAELLLPLAGPEVYARTSASQRAEKSIETVR
                     EMSAEYSRICGLPTKLREVGVKKEDFSAIARAAVNDGAMIVNPVEADEDDVLGILEKA
                     Y"
     gene            4431..6335
                     /locus_tag="PLM83_03060"
     CDS             4431..6335
                     /locus_tag="PLM83_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOK70422.1"
                     /translation="MEGRYRGYAGETLLIDLGSRQVSTYPWTDLERELYLGGKIMAAK
                     IMHDIVDPEVEPFSPENPLVVTTGPLTGSGAPSSSRFNISTLSPLTNLLTSSNCGGNF
                     GFHLKKAGFDGVIITGKSPEPVWIEIDDGKVVFHDAGELWGKTTGETQEALPKNAGKM
                     VIGPAGENLVLYASIVSQERVAGRAGVGAVMGSKNLKAVTARGSRRMVLYDEEKAKEI
                     FKEWVKALRGHPLTGVQLPQLGSAGLVAPMQYNKILATRNFARGRFDEFEAVSGETLR
                     EKHLIRNKGCITCPIQCGRVVEVDGKEVKGPELETLGLLGPNLENSNLELILEWNYLL
                     DELGMDTISAGSTVGFAMELNEKGLWDNGLAFGEVDNLADVFRQIACREGIGDLLADG
                     TKRLSERFGGREFAIHSKGLELSAYEPRRSVGMGLGYATSNRGGCHLNGGYLVLLEGL
                     ALNIDPLTTNSKAALTILFQNIMEAVSAGGSCLFTCYLAMPAFMLEDANAWTTRWINR
                     ALTRHGHLMGAVAKMPGWMMPFHFPGLWHTQALTAVTGMKMTFGRFKEIGERGYNLER
                     MFNAKRGVTAADDALPKRLTDDLQDPEVPGSRVPLDDLKKEYYKLRGWDFNGLPKKEK
                     LRKLGLAGVD"
     gene            6339..6596
                     /locus_tag="PLM83_03065"
     CDS             6339..6596
                     /locus_tag="PLM83_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOK70423.1"
                     /translation="MIEVRIYGPLRERLGTGSLEVEASRAGSVGELLAVVSEVTGAAS
                     LSELRKASVFVNGTNIVDLRLFRTALLPGDEVVLLSPSGGG"
     gene            complement(6860..8722)
                     /locus_tag="PLM83_03070"
     CDS             complement(6860..8722)
                     /locus_tag="PLM83_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOK70424.1"
                     /translation="MKLSIRKKLIFSYVIVLALAVGVAAAGIWSNQRTAQRYTDIIKL
                     SLPLETQILTVRAHMLERSVVLRDYILTQDEFCISEYESINESIDKALQAMDDYDLHA
                     QSIEYRDAIMDFESMYRDMANRMFIAISDGSVDQAVNLLIDEETFANAALTDSINSWR
                     DYIVNVNDEWIAEANRIARNGLIIIGICLVIALAAVIALTIILSKNIAVPVERIQAAA
                     DLMSMGDLTVAIPSIETGDEIEALNESVRRMTENLASLVREVQGTAEQVAGSSSQLSA
                     AAGESAQAVDQIAATVQQMANAAEQESSSANETAAASEQIMASVNQVASGAETQMMQV
                     KNTSELVDRMTEELSKVTGFLKELQKDMEITVKSAEEGNASVQEVEYIMNQIRQASAE
                     VESAAKGLDESSRQIGQVVQVISDIADQTNLLALNAAIEAARAGEQGRGFAVVAEEVR
                     KLAEGSLEETKTISKLIERTVADTGRVAKAIVASGELIEKSVPVVSAATASLEVIRKN
                     AQNNLAVAGSTATLGEALMNDTIQVNQGMSQVVAVSDENAAAAQQMAAGMAEVQKSVE
                     NIAAISEENAASIEEVSASSEEVNASIREIHAASELMADMAHKMMNDVAKFKVA"
BASE COUNT         1985 a         2190 c         2630 g         2349 t
ORIGIN      
        1 gtggtcgttt cttaggggga gtaacacgca cccggtcatt accccccgaa actgactcca
       61 tgttaatcac tcctcctccc gggcgtactg tacgtagctg gctgacccgg gctttccgtg
      121 ttggggtggt gagtggccca ctggcggtca ttctgcgaac gaaatgaatg ccagagagtc
      181 gtacttcgcc taaattgacc ctttgcgggt catttatcag gtgagctccg gaaactctcg
      241 tccatcccca tccaactgat atatgttcga gcaggcatct cgggctggtg tctaagcgcc
      301 ttcgccgtac atgattatca caaactcaag tccatcttcg tcatctgtcc agaagctgtg
      361 ggcggtgttc gccggtatta tgtatgcttc tccttctgcg aacctgtatt ctgtgtgtcc
      421 gagctttatg gagccctctc cttttgtgcc gatgacaaga gtgttgaagg gattgtactg
      481 gtcgtcctct ttttcgttga tgaccatgcc ctgttccact ctgtaccagg ctgttctgag
      541 gcctgaagta tataccaaag gcactgcgta tccgccatgc accaccctgg cgtggtcctt
      601 gccaaatagt atcttctccg gttctccgat tacgaagaag tgaaatgcga aatccattag
      661 gtccaagtct tttggcgggc tatagccatc gatgaacctg acatcgagcg gggcgtagtc
      721 gttcatcttg gctcttcctg cggcggttag gaaatgcatc ttccccaagt agatgtcttc
      781 cagtgtttta gctgtgctca caaggtagaa tgtgggcttg gaaggcttgc agttcgcaat
      841 cgtgtagctg ccttccttct gtatagagac cgtccaccat tggccgtcat tgatgaggaa
      901 accgaaggag atgtcaaccg ggtttggggt tttggcgatg taggacgtgc agaaaccctc
      961 caacatctcc cgaactggca gattggcgct cttgtcaatt tccatgtgga tcgctccttt
     1021 tgcatgagtt taagctcttt tttctcatct gaacatcgca ttcagatagg ccaccatgcc
     1081 gaggtcgccg tcgatctcga tgcgcccccg cgtcatgaat gcttcgggag agagaaagcc
     1141 tgtcatcagg ccttcaaaat cggctttgct caatgcaagc ctgaggtctg cctcttcctt
     1201 tgcgccttgt tctatctcca ctttgtgttg gccttttgaa acatgatagg ttatgccatc
     1261 atcggtgaaa catatttcga tgattccgtc cttcagtgtg cctgcgttgt gctccaattt
     1321 gcgttttgcc acttcgaaca gatcatcgag tgacttgttg ccgtgctctg gtgcttgagg
     1381 gccttcttca ggggcgagcg accacagatc ctccagtccg tcggtgacgg tcggagggag
     1441 gttgagcctt tcggccacta cgtatgccgc gatgtcatgc tggatccaca aggttattgg
     1501 catgatgatc cccggcaggg ttgaggagag tccgtaggta tcgtctggtc cggcaatctg
     1561 cgccatgagg ccctcgtcct ggtctgctac aaggcccatc catctgccat acaccgctgc
     1621 ttgcaggtat tggagggggc tgacgtagtg ctctacggcg tcctctacta tacctttgcc
     1681 ttccccgtaa acgagggtga aaactttgac gcccctcgat ttcgcttttg cgatttcgcc
     1741 agccaggcgg ggaaggtctt cggcaaatac cgatatgtac agccttctct gtgatcttgc
     1801 gcacattctc tctgcggttt ccgtcactag atcgtatcct gtgacgaccg aaaaagggtc
     1861 ttctgtccgc cctttgctaa gcctgtccaa ttcgacggag agataggtgg tgagatccaa
     1921 tagctcatcc tgaaatcggg ctataaggct gtggtgagag attggagtgt agtattgctt
     1981 cccgtcgact gatagtgaat atgcggcttt ttttcgcacc aatgactcaa gggtttcgta
     2041 tactttggct cgtggcaccc ccgacaattt gctggcctcg tatcctgtaa tgcttggctg
     2101 tttgacgagg gcagcgtata tcttggcttc gtattcagtc aggcccattt gctgaagacc
     2161 gcttatcaga tccatggcaa cctcccagcg gatagtaact actaaagtag ttactaaact
     2221 gccatttcat tatctatcag cgccaagcag atgtcaagca ctagaacttg cgttttatgc
     2281 ctaggagccg atatgtaaat tgaagggatt tgaggtagag tagtacttag ttgacatgta
     2341 tatgtgccgt gatacattct aatcagctgg gggaaatacg gatgggggag gtggcgtgac
     2401 aggattgagg cgtcttttca aggaagacaa gctatatata ggcaactcgg actggagctg
     2461 agagcatccg ctagttccga taatgaggat gctcattagt ggcctgctga ttgtgtggta
     2521 gggtgttgcc gagcacgata gtgatcagac gggcaactgt cactttggtg tgcagataaa
     2581 tcgctaaaac ggggtgttct ttcccgcgtt gtccgcctat atccagctat gcagcgggca
     2641 cgatgcgcgg tatgggcgag agcgagtata cacaaaggaa agagaggggt cagcgatttg
     2701 gccagcgctt atcgggagat aaggccaccg gcaaagaggt ttgagagaag ctgtgtttcg
     2761 gttctgctgt ggtttgtggg cagagggttt caggcggccg cccatgtaga caaacatgtg
     2821 caggacgagg tgtgcggatg gaaagatggg actattatcg ctataggagt tgcgccttgc
     2881 ggcccgcaca tgagcgtcag aaaaactggc aacaggttgc gtttcctcgg caatgtgaaa
     2941 gctccggacg cggacgctgt catcgaattc aagagcctcg atgccgctct gctcgtcctt
     3001 accggacaga tcggaatcga ccgggcttac gcggaacata gattctcttt gaaaggggat
     3061 ctgttttcca taggcatgcc gctcgttagg tgcctctaca ttgtggagtc gtacctcttc
     3121 cccaaaagca ttgccaagaa gattctgaag agatggccaa agaggagttc ctctgggttt
     3181 gcagtctatt tccacaccat tttcggggtg taatagaagg aggctgaaga atgattcctt
     3241 cttattatga attcataaac tctgtgaaga tactatcagg caagaatgct cttgagaata
     3301 ttccggcaga actcagggga ttcgaggccc gaaagccaat cctgctcacc gacaagatgc
     3361 tggtcaagat aggcctggcg cagcaggtcg tagatgcgtg cggcggatct gaaattgtga
     3421 tcggcgccat gtatgatgat atcccccctg attcctcagt ggaagtggtc aatgacatag
     3481 tccgtgttta tcgcgaaaag ggatgcgatt cggtcattgc tgttggggga ggttcggtga
     3541 tagataccgc taaaggcgta tgtatttgct taaccgaggg gactgatgac cttctcgacc
     3601 acatgggtgt cgatattctg aggagacgta agacgccttt cgtggtagtg cctaccactg
     3661 cggggacagg ttccgaggtt accggggtcg ctgtgatcgc aaaccctgca aagaatgtga
     3721 agatggaatt tgtatcggcc aacattctgc ctgatgttgc tgtcctcgat gtgaggatga
     3781 caaagtcgct cccgccacgt ctcactgcgt ccactggcat ggatgctctt gtgcatgcta
     3841 ttgaggccta tacgtgtctg cagaggaatc ccttgagcga tgcacatgct catgcggcca
     3901 tcaacctgat ccggaagtat ctgccccgag cagttcgaga cggcaaggac gaggaagccc
     3961 ggcttgccat ggccaatgca tcgcttctgg caggtgtcgc tttctccaac tccatggtgg
     4021 gcatagtcca tgccgtgggc catgcatgcg gaggggtgtt gcgggcgcca cacggagact
     4081 gcatggcgat attgcttcct tacgggatgg aatataatct gagtgcagtg tcggagcgct
     4141 atgcggaact ccttctgcca ttggctggac cggaagtata tgcgcgaact tctgcgtcgc
     4201 agagggctga aaagtcgatt gagactgttc gggagatgag tgcagaatac agcaggatct
     4261 gtgggcttcc gactaagctg agggaggttg gcgtgaaaaa ggaggatttt tcggcaatag
     4321 ccagggcggc tgtgaatgat ggcgctatga tagttaatcc ggttgaagcg gatgaagacg
     4381 acgtgctggg catcttggag aaagcctatt gatgggaggc tggagaggct atggagggtc
     4441 gatatagggg ctatgcagga gagactttgt tgatagactt gggttcgcgc caagtgtcaa
     4501 catatccatg gaccgacctg gaacgggagc tgtatctggg cggcaagatc atggcggcta
     4561 agataatgca tgacatagtg gatcccgaag tggagccgtt ttcccctgaa aacccgctgg
     4621 ttgtgaccac aggccctctc actgggtcag gggcgcccag ctccagcagg ttcaacatct
     4681 ccacattgtc gcctctcaca aaccttttga catcgtccaa ctgcggcggg aatttcgggt
     4741 tccacctgaa gaaggctggg ttcgacggcg taataattac tggtaaatct ccggaacctg
     4801 tctggataga gatagacgat ggcaaggtcg ttttccacga cgccggagag ctttggggaa
     4861 agacgacagg ggaaactcag gaggctcttc cgaagaacgc agggaagatg gtgataggcc
     4921 cggctggcga aaacctcgtg ctttatgcat ctattgtgag ccaggagcga gttgccgggc
     4981 gggcgggggt tggggcggta atggggtcga agaacttgaa ggctgttact gctcgcggaa
     5041 gcaggaggat ggttctttac gatgaggaga aggcaaagga gatattcaaa gagtgggtca
     5101 aggcactcag ggggcatcca ttgacagggg tgcaacttcc tcaactgggg tcggcggggc
     5161 tcgtggcccc gatgcagtac aacaagatac ttgcgacgag aaactttgct cgcggccgat
     5221 ttgacgagtt tgaggccgta tccggagaga cgctgcggga aaagcatctt ataaggaaca
     5281 agggatgcat tacgtgcccc atccaatgcg gaagggtggt ggaggtggac ggcaaggagg
     5341 taaaaggccc tgagctcgaa actttggggc tgctcgggcc gaacctggag aactccaatc
     5401 tcgagcttat tctggaatgg aactatcttt tggacgagct cggcatggat accatatccg
     5461 ccggcagcac tgttggattt gccatggagc ttaatgaaaa gggtttgtgg gacaatggcc
     5521 tcgcctttgg cgaggtggac aaccttgcag acgtgttcag gcagatcgcc tgcagggaag
     5581 ggattgggga tcttctggcc gatgggacca agcgcctttc cgagaggttc ggcggccggg
     5641 agtttgctat acactccaag ggcttggagc tgtcagcgta tgaaccgcgg cgttcagtcg
     5701 ggatggggct gggctatgcc acatcgaaca ggggaggatg ccatctaaac gggggatacc
     5761 tggttctcct tgaagggctg gcactcaata tcgacccgtt gacgacgaac tcaaaggcgg
     5821 cgttgaccat tctttttcag aacattatgg aggcggtgtc ggcgggaggg agctgtctgt
     5881 tcacgtgtta ccttgccatg cccgcgttca tgctggagga tgcgaacgca tggacaacta
     5941 ggtggatcaa tcgcgcactc actcgccacg gccatctgat gggagctgtt gcgaagatgc
     6001 cgggctggat gatgccgttt cactttcctg gcctttggca tacgcaggct cttactgctg
     6061 taaccgggat gaagatgacc ttcgggaggt ttaaggagat aggagagaga ggatataatc
     6121 tcgaacgcat gttcaacgcg aaaaggggag tgacggcggc agatgatgcg ttgccgaaac
     6181 gcctcaccga tgacctgcag gatcctgagg ttcccggaag cagggtcccg cttgatgacc
     6241 tgaaaaagga gtactacaaa ctgagaggtt gggattttaa cgggctgccc aagaaagaga
     6301 agctgcgcaa gcttggcctg gcgggggtgg attgattcat gattgaggtg cgcatctatg
     6361 gcccgctcag ggagagattg ggtacgggtt cgctcgaagt tgaggcatcg cgggcgggct
     6421 ccgtcggcga actgcttgcc gtggtaagcg aagtcactgg agcggccagt ctgtcggagc
     6481 tgcgcaaagc gtcagttttc gtgaatggga cgaatatcgt agatttgagg ctgttcagga
     6541 ctgctctgtt gcccggggat gaagtggtat tgctttcgcc gtcgggaggc gggtagaagg
     6601 caggagcggt gcccaaggtt cacctcaaaa agagatgaca aggcccttac gttcagatgc
     6661 cctcaggtct gtttggtgaa tcttgatatt gagtccggca tgatttttag ccggccttac
     6721 agttgcagaa ggtgttgggg attggcgatg tccccatcgg agcatggccg aacggttcac
     6781 ttggagaggc aaattgctaa tagaagagcc ctgcaggagc agggctctct aacacaacgt
     6841 agcctagttc aaagcgcaac taggcgacct tgaacttggc gacgtcattc atcatcttat
     6901 gcgccatgtc ggccatcagc tcggacgcgg catgaatttc acgtattgag gcgttcactt
     6961 cctcggagga tgcggatacc tcttcgattg aggctgcgtt ttcttcggat atggcagcga
     7021 tgttctctac ggatttttgc acttctgcca tgcctgccgc catctgctgg gctgcagcgg
     7081 cattttcatc ggatacggca accacttggc tcatgccctg attgacttgg attgtgtcat
     7141 tcatcagtgc ttctcctagt gtggctgtcg agcctgcaac agcaaggttg ttctgagcgt
     7201 tctttcgtat gacctctaaa gaggcggttg ctgctgatac tacaggcacg cttttctcta
     7261 tcagctctcc tgatgcaaca atagctttgg caacacgtcc agtatcagct actgttctct
     7321 ctatcagctt ggagatggtc ttggtttctt caagcgatcc ttctgcaagt ttgcgcactt
     7381 cttcagcaac taccgcaaaa cctcgaccct gttcgccggc gcgcgccgcc tctatggctg
     7441 cgtttagggc caacagattg gtttggtctg cgatgtcgct tattacttga acaacctgcc
     7501 caatctggcg tgagctttcg tctaggcctt tcgctgcgct ttccacttcg gccgaagctt
     7561 gtcggatttg gttcattatg tattcaacct cttggacaga tgcgttacct tcctcagcag
     7621 acttgacggt aatttccata tccttttgca gctctttgag gaaacccgtc actttgctga
     7681 gttcttcggt catgcgatct accagttcgg atgtgttttt cacttgcatc atttgagttt
     7741 cggcgcctga cgcaacctga ttcacgctgg ccattatctg ttcgctggcg gctgctgtct
     7801 cgtttgcaga agagctttcc tgctcggcgg cgtttgccat ttgctgcacg gttgctgcta
     7861 tctgatccac ggcttgggct gattccccgg cggccgctga cagctgggag cttgatccgg
     7921 caacctgctc tgcagtgccc tgaacttcgc ggacaaggct tgcgagattc tccgtcattc
     7981 gacgcacaga ctcatttagg gcttcaattt cgtcccctgt ctcgatcgat gggatggcga
     8041 cagtaaggtc acccattgac atgaggtctg ctgctgcctg tattctttcg actggtacag
     8101 caatgttctt cgagaggatt atggttaggg caattacagc cgctaatgcg attaccaggc
     8161 atatcccgat gatgatcaga ccgtttcggg ctattctgtt tgcttctgca atccattcat
     8221 cgttcacgtt gacgatgtaa tcgcgccagc tgttgatcga atcggttagt gctgcgttgg
     8281 caaaggtttc ttcgtcgatg agcagattca cggcctggtc cacagagcca tcgctgattg
     8341 cgatgaacat tcggtttgcc atgtccctgt acatggattc aaaatccatt atggcgtctc
     8401 tatattctat ggactgggcg tgtagatcat agtcatccat ggcctgcaga gccttatcga
     8461 tgctctcatt gatggactcg tattctgaga tgcagaattc gtcctgggtg aggatgtagt
     8521 ctcggagcac gacgcttctt tccaacatgt gtgcacggac cgtcaatatc tgtgtttcaa
     8581 gagggaggct tagcttgatg atatctgtgt agcgttgagc cgttctctgg tttgaccaga
     8641 tgcctgcagc tgcaacgcca acggccagtg ctaacactat tacgtaagag aagatcagtt
     8701 ttttgcgaat gctcaatttc aaccgcattg cacctccatg gcttcgctta atacctttat
     8761 cggcaggttt gagcaggatc ttcacaggaa aaaatgtgtc ggaaggacac tacatggatg
     8821 gtatagaccg gatatagcca aacattcaag tgtgtgggga aaccaagata gatgttggta
     8881 ttatgcctgc aacgagttgc attccggctc ggggcgcggc atcacgcaag gagcaaatgt
     8941 tgtgaaaaac accaggattt cgagaaggac atatggcgca ggacgataat atgtaatgtg
     9001 actagaggcg tgccggtatg cgctttccgg tattagacag cctgccaaca cgtactatga
     9061 cgacacaaaa tggttccact cattctcaca cttgacatgc taaccaacct ggtgcattaa
     9121 gcagcttgaa ccggaaccgt gtgaattgcc cggt
//
LOCUS       DAOSAI010000050         9031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5046_length_9031_cov_4.052028, whole genome shotgun sequence.
ACCESSION   DAOSAI010000050 DAOSAI010000000
VERSION     DAOSAI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5046_length_9031_cov_4.052028"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="PLM83_03075"
     CDS             <1..1484
                     /locus_tag="PLM83_03075"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOK70425.1"
                     /translation="ANKVYAAADWEWMMNEGDTLSMGWKPEIGFLSARWNSFNEGLLA
                     YVLAIGSPTHPISASAWDNIFRPVNENYICLPQETLFVYQYPAAWIDFRNKEDSYANY
                     FNNAVTATRMNRLFAVLRRFNYSTYDMDIWGISACDGPAGYKPYGASEGNHDGTVAPY
                     ASIASMPFTPELSMAAIRAMLEREGGLVWGEYGFVSGFNVDQNWYSDQYVGIDQGIIL
                     LMLENYRSELIWKHFMAHPAIEEAMERIGFVKSDSDYAVTPAYLDAWERMRLAPSEKK
                     AVAKRAATPVVIDGDLSEWKGLEGYVVDEDMNVPAGGIEKVDKKKQVLNSTFYVQYDE
                     EYLYIAANVADEYIVINIKPEDKAGYYRTDSVEFYIDPSRAGSGAGLMKLAILPFDTE
                     GNVQAVRHEDTKPGPIAKTNPRIRVASSRTERGYAIEAAIPLADLGIKAEPGTKLGFC
                     HTVHNSNDREAKIGQYVRTNVIAWNNLPEIWANPDLWGELVFE"
     gene            1538..2752
                     /locus_tag="PLM83_03080"
     CDS             1538..2752
                     /locus_tag="PLM83_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOK70426.1"
                     /translation="MRRAASMILCGALLLGIMVSSAAAAPVRLTVWAMGAEAQKIGEM
                     TAKFEQANPDIRVTVQAIPWSAAHDKIITAVAGGTGPDVAQMGTTWMAEFGSIGVFEE
                     LSRYMSKSKVVDKSQFFSGSYGTCVVGGKVYGVPWYVDTRALFYRKDLLAQKGFKQPP
                     QTWDELRAAAAALAADGKGYGMALSTNNYQEFLPFVWQNGGRILDEKGRPQVTEPEFV
                     EALDFYAKLFHDKIAPIDSGGSDLFQEFAKGTMPMYFSGPWMINLTREQTPEIDGKWS
                     VALCPKKKTRTSFVGGCNLVVFKDSRNKDAAWRFVEFMSDPRTQLEWFRVSGCLPAVK
                     KSWDARDLAGNPMMAVFREQLDDARAPENVPQWAQIEVAVQRRVQEACYGKKTAMEAA
                     RALEADIKAIMR"
     gene            2952..3833
                     /locus_tag="PLM83_03085"
     CDS             2952..3833
                     /locus_tag="PLM83_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK70427.1"
                     /translation="MQRNAKVAYMFLAPALVLMMVFVVIPVVASFVISLTNFDIFALA
                     DWSNAKFIGFDNYVRLFQDELFWKALSNTLYFVAVGVPLAIVLSLILAVLLNSPFNHA
                     KDLFRVGYYLPSITETVAIAVVWRWVLNPRYGILKRALEIFGLPSPNWLGDPRWAMPA
                     IIMMAVWKGLGHNALIFLAGLQSIPSTVYEAAQIDGANGWEQFRFITLPLLAPTTFFV
                     AIMTLIGYLQLFAEPYTLTDGGPLDATLSIVLYMYRHGFKFFNLGYASAMAYVLFAMI
                     FVATLLSTRSRAEEVTY"
     gene            3883..4713
                     /locus_tag="PLM83_03090"
     CDS             3883..4713
                     /locus_tag="PLM83_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK70428.1"
                     /translation="MKAARNIPMTVLVNFLLIVGLVGMAAPFVWMLSTSFKGTKDIFR
                     YPPQWIPQEPTLDNYRKLFRTVNIGRPLINTVITAVSITGLSLIICSMAGYAFAKFEF
                     PGKNVLFMGVLGTLMVPGQITMIPVFLLLKKLGLLNTYSGLVLPGIASAFGIFFMRQF
                     MVTLPNELLEAARIDGAGEFFIFNRIVLPLCKPALATLGILNFTGSWNNFLWPLIIST
                     DESMYTLPVAVANLAGQYATEYGLQMAGSVMVIMPIVIFFLFAQQWFMEGVAMTGMKG
                     "
     gene            complement(4786..5055)
                     /locus_tag="PLM83_03095"
     CDS             complement(4786..5055)
                     /locus_tag="PLM83_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70429.1"
                     /translation="MNQFTLHLDKEFLDAVERTVAEACEEMRRSSKRNSAGANWAFFA
                     KSAWDEAIEEAIGHVLDQRAGSVLGLMRPASQQVARSRDRTLNRL"
     gene            5368..>5903
                     /locus_tag="PLM83_03100"
     CDS             5368..>5903
                     /locus_tag="PLM83_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70430.1"
                     /translation="MSFIAGFVCRRPWVVIAAVIAITCISLLGTMRIRVSSGLREFYA
                     QTDPQIKSMDLIEETFGGSEYIMIAFPSEQVFTVRGLRALYALSEGMEEIEGIARVRS
                     ITNIAEVTGTPWGLEVSQTLEDMPETEEEAEAFRVRIEESSEISGALLSGDGDHVLTL
                     VGLSPGADRRQHRIAGYC"
     assembly_gap    5904..5913
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5914..7809
                     /locus_tag="PLM83_03105"
     CDS             <5914..7809
                     /locus_tag="PLM83_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOK70431.1"
                     /translation="PGADAGRVAKSVQEATGRLAPDFSPHYAGGPVLAGAADEFLKTD
                     IKKLLPVCTIVIVVVLFASFRTVAGVVLPLATVMLSVVWTMGLMGFADIPFTQITSAL
                     PVVLLATGSAYGIHIMHRYYENHESGADGSSAASSAVESVGVAIVLAGITTIAGYGSN
                     AASSVVRIREFGLLAGFGVSAGLVIALTFVPAVLSIFRPAPRKVALEGSGCPGGGCPP
                     VVARIGRYGSTVNDSSGCASKEHPVGAGEAERSWRDSLFTALSRLVARRGWVTGLIAA
                     ALAVFALAGVARIQVDTSPVTFFPSDSVERRDFDLIRSEFGGVDTVQIMVGGDILDPK
                     TLSAIERVHGEFESVGGYGRALSIVNVLKQASRALHDNDKAWERLPSTRKEAAQYVVL
                     LSMSGDVGLDEMITIDNRKARIEVMADTVDNSTNKASRLQSAVEIVKANLSGVSTISS
                     LSITGMPFLEQSMGEMVMSSQKMSLLFAAIAVAFIVYMSFGRSWDSFLCLLPVALTIL
                     ANFGMMGWAKISLNLATALVSSIAVGIGIDYSVHVYSRFRQGIMEGYSPEDAMRATIA
                     NTGRAVALNALAVAAGFAVMLLSEFPPLQQFGALIAFTMVVSAGGALMILPASLLAVA
                     RRRSLAP"
     gene            7837..8763
                     /locus_tag="PLM83_03110"
     CDS             7837..8763
                     /locus_tag="PLM83_03110"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOK70432.1"
                     /translation="MTSQSFPGSGRSNVLSKHAARSSQRVDLSRLGICRGKVGALAGI
                     AIVLFAAVACAALLCAGAVAAAGLQVRGDLTAKQILDSIEGKGSLAGNGCAVVEMVVE
                     RGKSQKLNRVEVMRSDDGNGTIKQLVEFLAPADVRGMKFLSITAPGEEDQMWLYLPAV
                     GSERRIAGSAASGKFMGTDFTFEEISASAKTWEPYKPERMPDDTVDGQKCYVLRLMPE
                     SGNLPYGAVTIWIWQENVLPLRIEFFNNSKKLQKVMTFSDLKKNKSGEWQPHTIVLQD
                     ILGGSLTTVRILEMDDRPVPDEVFTLRYLRKR"
     gene            8844..8972
                     /locus_tag="PLM83_03115"
     CDS             8844..8972
                     /locus_tag="PLM83_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70433.1"
                     /translation="MTALLIAVIVCTCASHAALASEWDPPEITGSAEWQIDYLPGE"
BASE COUNT         2022 a         2164 c         2712 g         2123 t
ORIGIN      
        1 tggcgaacaa ggtttacgcg gccgcggact gggagtggat gatgaacgaa ggcgacaccc
       61 tgtcgatggg ctggaaaccg gagatcgggt tcctctcggc aagatggaac tcgttcaacg
      121 aaggcttact cgcctatgtt ctggcgatag gctctcccac gcatcctata tcggcttcag
      181 catgggataa catcttccgt ccggtaaacg aaaactacat ctgtcttcct caggagacgc
      241 ttttcgtcta ccaataccct gctgcatgga tcgatttccg caacaaagag gacagttacg
      301 caaactactt caacaatgcg gtgacggcca caaggatgaa ccgcttgttt gccgtgttgc
      361 gcaggtttaa ctattcaact tacgacatgg acatatgggg aatctccgca tgtgacggac
      421 ctgcagggta caagccttat ggtgcatccg aaggaaacca cgatggcacc gtggctccgt
      481 acgcgtccat cgcatcgatg ccgtttacgc ctgagctttc tatggctgca atcagggcca
      541 tgctagaacg tgaaggcggg ctggtgtggg gcgagtacgg cttcgtgtcg ggattcaacg
      601 tggaccagaa ctggtattcc gaccagtatg tggggataga ccaagggatc atcttgctga
      661 tgctggagaa ctaccgttcg gagttgatct ggaagcactt catggcccat cctgccatcg
      721 aagaggccat ggagagaatc ggattcgtga aaagcgattc ggattacgca gttaccccgg
      781 cataccttga tgcatgggag aggatgcgcc tcgctccttc ggaaaagaag gcggtggcga
      841 agcgggccgc cacgccagtc gttatagacg gggatctttc ggaatggaaa ggcctcgaag
      901 gctacgtggt ggatgaagac atgaatgttc ccgccggtgg catagagaag gtcgacaaga
      961 agaagcaggt gctcaattcg actttctatg tgcagtatga cgaggaatac ctgtacattg
     1021 ccgcaaatgt ggcagacgaa tacatcgtaa tcaacatcaa acccgaggat aaggcgggct
     1081 actaccgtac agactcagtc gagttctata tagatccttc aagggctgga tctggtgctg
     1141 gattgatgaa actcgccatc ctgccttttg acacggaagg caatgttcag gctgtgcggc
     1201 atgaggacac caagcctggc ccaatcgcca aaacgaaccc ccggatccgt gtggcttcga
     1261 gccggacgga gcggggatac gcaatagagg cggcgatacc ccttgctgat ctggggataa
     1321 aggctgaacc agggacgaaa ctcgggttct gccatacggt gcacaatagt aatgacaggg
     1381 aagcgaagat agggcagtac gtgagaacta acgtgattgc gtggaacaac cttcccgaga
     1441 tctgggccaa cccggacctg tggggagaac tcgtattcga gtagcgatag attgaggaga
     1501 gcggcgtcga ctgtataaat caaggaggga catgaatatg cgaagagcgg catcaatgat
     1561 tctgtgtggc gcgttgcttc ttgggatcat ggtgtcatcc gctgctgcag cccctgttag
     1621 actgacagtt tgggcaatgg gtgcggaggc tcaaaagatc ggggagatga ccgccaagtt
     1681 tgagcaggcc aatcccgaca ttcgcgtgac agttcaggcc ataccgtgga gcgcagctca
     1741 tgacaagatc atcactgcgg tggcaggcgg aaccgggcca gatgttgccc agatgggcac
     1801 cacgtggatg gcggagttcg gctccatagg agtattcgaa gagctgtccc ggtatatgtc
     1861 gaaatcaaag gtagtagaca agagccaatt cttctcagga tcttacggca cgtgcgttgt
     1921 gggcgggaaa gtgtacggtg tgccttggta cgtagatact agggccctgt tttacaggaa
     1981 ggacttgctt gcgcagaaag gcttcaagca accccctcag acctgggacg aactgagggc
     2041 ggccgcggcg gctctggctg cggacggaaa gggatacggc atggcccttt caaccaataa
     2101 ttaccaagag ttcttgccgt tcgtatggca gaacggcgga cggattctgg atgagaaggg
     2161 ccgaccgcag gtgacggaac cagagttcgt ggaagcgttg gacttctacg ctaagctctt
     2221 ccacgacaag atcgccccga tcgacagcgg cggcagcgat ctttttcagg agttcgcgaa
     2281 gggcactatg ccaatgtatt tcagcggacc atggatgatc aatctcactc gtgaacagac
     2341 gccggagatt gatggcaagt ggtcggtagc gctatgtccc aaaaagaaga cgagaacgtc
     2401 atttgtgggc gggtgcaatc ttgtagtatt caaagactcg cggaacaagg atgccgcgtg
     2461 gcgtttcgtg gagtttatga gcgatcccag gactcagctg gaatggttca gggtttcagg
     2521 atgccttcct gcggtgaaaa agtcgtggga tgctagagac ctagcgggaa atcccatgat
     2581 ggcggtcttc cgggagcagc tggatgacgc ccgtgcgccg gagaacgtgc cgcaatgggc
     2641 ccagatagag gtcgctgtac aaaggagggt gcaggaggcg tgctatggca agaagactgc
     2701 catggaagct gcccgtgctc tcgaagcgga tataaaggcg atcatgaggt agatcacgtg
     2761 ggcaagtgag gcggaactcg ccgccacgct cacccacgcg tagcctgcgc cgaggggcat
     2821 gaacctctcg gcgcaggcga tccggccctg atcttgaatt ttggatccgg ttttggtttt
     2881 cgttattagc cgcgctgcgt gaagccgatt gctggctggc aaggcatgtg cggcgtttgg
     2941 aggtcgttgc catgcagcga aatgctaagg tggcatacat gttcttggcg cctgctctgg
     3001 tattgatgat ggtgtttgtc gtaataccgg tggttgcttc tttcgtgata agcctgacca
     3061 atttcgacat ctttgcgctg gccgattgga gcaatgctaa gttcatcggg ttcgacaact
     3121 atgtccggct tttccaggac gaactgtttt ggaaggcttt gagcaacacc ctttacttcg
     3181 tggcagtggg agttcccctc gcaatagtgt tgtcgcttat tctcgcagtc cttctcaact
     3241 ctccattcaa ccacgcgaag gacttgttta gggtgggata ttaccttcct agcattactg
     3301 agactgtggc catcgccgtg gtgtggagat gggtcctcaa tccgaggtat ggaattctca
     3361 aacgtgccct ggagatcttc gggttgcctt cgcccaactg gctcggggat ccacgatggg
     3421 cgatgcctgc aataatcatg atggcggtgt ggaaaggact gggtcacaac gctctgatat
     3481 ttctcgccgg ccttcagagc atcccgtcaa cggtatatga agcagcgcag atcgatgggg
     3541 caaacggctg ggagcagttc aggttcatca cattgccgct attggcacct accacgtttt
     3601 ttgtggcgat aatgactcta atcggctacc tgcagctctt tgccgaacct tacaccctta
     3661 cagacggtgg gccattggat gcaaccctgt ccatagtgct ttacatgtac aggcacgggt
     3721 ttaagttctt caaccttgga tatgcctctg ccatggctta cgttctgttt gccatgatat
     3781 tcgttgccac actgctatcg acgcggtcaa gagccgaaga ggtgacttac tgatgaggga
     3841 gctgtttgaa tcgttaaagg acaggaatgg agggaatcgg ggatgaaggc agcacgaaac
     3901 attcccatga cggtcctcgt caacttcttg ctcattgtgg ggcttgtggg catggccgcg
     3961 cctttcgtgt ggatgttgtc gacgtcgttc aaagggacga aggacatctt ccggtaccct
     4021 ccccaatgga taccccaaga gcccacactc gacaattaca gaaagctgtt cagaacggtc
     4081 aacatcggca gacccttgat aaatacggtg attactgccg tatcaatcac tggactttct
     4141 ctgattatct gctcgatggc gggatacgct tttgccaagt tcgagtttcc cgggaagaat
     4201 gtgcttttca tgggcgttct cggaacgttg atggttccag ggcagatcac catgatacct
     4261 gtcttcctcc tcttgaagaa actagggctc ctaaacacct attcgggcct tgttctgccc
     4321 ggcatagcca gcgctttcgg gatatttttc atgagacaat tcatggtgac gctgccaaat
     4381 gagcttttgg aggcggcgcg gatagacggg gcgggggagt ttttcatatt caaccgcata
     4441 gtattgcctc tgtgcaaacc ggcccttgcg accctgggaa tcctcaattt cactggctca
     4501 tggaacaact tcctgtggcc gttgatcata tcgaccgatg agagcatgta tacattgcct
     4561 gttgccgttg ccaatctggc agggcaatac gcgaccgaat acggattgca gatggcaggc
     4621 tctgtgatgg tgataatgcc tatcgtgata ttcttcttgt ttgcgcagca gtggttcatg
     4681 gaaggggttg cgatgacggg aatgaagggc tagaactggt tgaggttgag cagtcgggac
     4741 ttggtgcaat tgtctcacgc taagcacgac gctcaacccc tatcttcaga gccggttcaa
     4801 tgtccgatcc cgactgcgag caacttgttg agaggccggg cgcatcaatc ccagaacgct
     4861 gcctgctctt tggtcaagaa catggccaat agcttcttcg atcgcttcat cccaggcaga
     4921 cttcgcaaag aacgcccagt tcgccccggc gctgttcctc ttggaagatc tccgcatctc
     4981 ttcgcaggct tccgccacgg tgcgttcgac ggcatcgaga aactccttgt ccaagtgcag
     5041 cgtaaactgg ttcacggatg ttccaccttt ctctgttgca gcatggaaat cgtcgtttgc
     5101 attatccaag ggcacgagct acagggagcc gtaggcttcc ggcgactgcg ttgtgccacg
     5161 tttcatctcc attagaattc tctcagcggc tgtgaatgtc cttcttggat aatggtaatt
     5221 gcgccgactg cacgtgcata tctgtgtatt gacagagagg tgaaacaacc ctatcatatg
     5281 actgactggt cagtcatatg atagggcggt taaccaacgc aatgcttcaa aaccctgaca
     5341 aaggggttac taggatggag tgagataatg agtttcattg caggattcgt gtgccgccga
     5401 ccgtgggttg tcattgccgc tgtaatcgcc ataacgtgca tatcactctt aggcacaatg
     5461 cgcatcagag tcagttccgg actacgtgaa ttctacgcgc agacagaccc gcaaatcaaa
     5521 tcgatggacc tgatagaaga gactttcggc ggatccgagt acatcatgat cgccttccct
     5581 tctgaacagg tgttcactgt aagaggcctt cgggctttgt acgcactctc cgaggggatg
     5641 gaggagattg aaggaatagc aagggtgagg tcgattacca acatcgcaga ggttacaggt
     5701 acgccgtggg gcctggaagt ttcccagaca ctcgaggaca tgcctgaaac cgaggaagag
     5761 gcggaagcct ttagggtgcg aattgaagag agcagcgaaa tatcgggcgc cttgctttcc
     5821 ggagatggag accatgtcct tacccttgtc gggctcagtc ctggggcaga caggcgccaa
     5881 caccggattg cagggtactg tgannnnnnn nnncctgggg cagatgccgg tcgggtcgcc
     5941 aagtcggtgc aggaggctac tggaagattg gcaccggact tttcccctca ttatgccggc
     6001 gggccggttc ttgccggtgc tgcagacgaa ttcctcaaga cagacataaa gaagcttctt
     6061 ccagtgtgta caatagtgat cgtcgtcgta ctctttgcga gcttccgcac tgtggcaggg
     6121 gtggtgttgc cgcttgccac tgtgatgctg agcgttgtat ggaccatggg cctcatgggg
     6181 tttgccgaca tcccgttcac acagatcacg tcagcccttc cggtggtcct tcttgccaca
     6241 ggaagcgcgt acggaataca cattatgcac agatactacg aaaaccacga aagtggcgcc
     6301 gacggcagtt cggctgcgag ttctgctgtg gaatcggtcg gtgttgctat tgtgctggca
     6361 ggcattacca cgattgcagg ctacggctct aatgccgcca gcagcgttgt gaggattcgc
     6421 gaattcgggc ttcttgcagg gttcggcgtg agcgccgggt tggtgatagc attgactttc
     6481 gttccggcag tcctgtccat ctttcgaccc gcccctcgca aggttgcgct ggaggggagt
     6541 gggtgccctg gaggcggctg tccccctgtg gtcgcccgaa tagggcgata tggctctacg
     6601 gtaaacgata gcagtggttg cgcttccaaa gagcatccag tgggggctgg agaagctgag
     6661 cggtcgtgga gggactcatt gttcacagca ctcagccggt tggtggcgcg ccgagggtgg
     6721 gtaaccgggc tgattgccgc agcacttgcg gttttcgccc ttgcaggtgt tgcccgcatc
     6781 caggttgata caagcccagt cacattcttc ccatccgaca gcgtcgaaag gcgcgacttc
     6841 gatctgattc gatcggaatt cggcggcgtt gacactgtac agataatggt tggcggcgat
     6901 atcctggatc ccaagacctt gagtgcgatc gagcgcgtcc atggagagtt tgagtcggtg
     6961 ggcgggtacg gccgagctct gtcaattgtg aacgttctca aacaggcatc ccgagcgttg
     7021 cacgacaatg acaaggcctg ggagaggctg ccttccacac gaaaagaggc tgcccaatac
     7081 gtggtgttgt tgtcgatgtc aggagatgta gggctagatg aaatgatcac catcgacaat
     7141 cgcaaggcca ggattgaggt gatggcggat actgtagata actcgacgaa caaagcgagt
     7201 aggctccaat cggcggtgga gattgtcaag gcgaatctgt cgggagtgtc gactatttcg
     7261 agcctttcca taaccggcat gccatttctt gagcagagca tgggggagat ggtcatgtcg
     7321 agtcagaaaa tgagtttgtt gtttgcggca atagccgtcg cattcatcgt ctacatgagc
     7381 ttcgggcggt catgggattc cttcctttgc ctgcttccag tggcccttac cattctggcg
     7441 aatttcggaa tgatgggatg ggctaaaatc tctctaaacc tggcgactgc cttggtgagc
     7501 tctattgcag tgggaattgg aatcgactat agcgtacacg tctattctcg tttcaggcaa
     7561 gggatcatgg aggggtatag tcctgaggac gctatgcgcg ctactatagc caatacagga
     7621 agggctgttg ctctgaacgc gttagcagtt gcagcaggtt ttgcggtcat gttgctatcc
     7681 gagtttccgc cacttcagca gttcggagct ttgatagcct ttactatggt ggtatccgcg
     7741 ggcggagctt tgatgatctt gcccgccagc cttctagctg ttgcgcggag gcgcagctta
     7801 gcaccgtaag tctgttcttt aaaggagggc attgatttga catcccagag tttccccgga
     7861 agtggcagga gtaatgttct ctctaaacat gcggcacggt cctcgcagcg ggttgacctg
     7921 tcccggctgg gcatttgccg gggcaaggtg ggagccttgg ccggaatcgc aatcgtgctt
     7981 tttgcggcag tagcgtgcgc tgctctattg tgtgcggggg ctgtcgcagc ggctggcttg
     8041 caggttagag gagatcttac ggccaagcag atcctggact ctattgaagg gaagggatct
     8101 cttgccggca atggctgcgc cgtggtggag atggttgttg agaggggcaa gagccagaag
     8161 ctgaaccggg tagaagtgat gaggtcggat gatggcaacg gcacaatcaa gcagcttgtg
     8221 gagttcctag cgcccgctga tgtgaggggg atgaagtttc tgagcattac ggctcccgga
     8281 gaagaggatc agatgtggct ttacctccct gcggtgggca gcgaaagacg aattgcaggc
     8341 agtgctgcat caggcaaatt catgggaacg gacttcacct ttgaggaaat ctctgcaagc
     8401 gcgaaaacgt gggagcctta caagcccgag cggatgccgg atgatactgt ggatgggcag
     8461 aagtgctatg tgctacggtt gatgccagaa agcggaaact tgccgtatgg agctgtaacg
     8521 atctggatct ggcaggagaa tgtcttgccg ctgaggatag agtttttcaa caattcaaag
     8581 aagctccaga aagtgatgac cttttccgat ctgaagaaga acaagagcgg agaatggcag
     8641 ccgcatacca tagtccttca ggacattttg ggagggagcc tgactaccgt tcggatactc
     8701 gagatggatg ataggcctgt gccggatgag gtatttactt tgagatattt aaggaagagg
     8761 tgaagcttgt gaccagtggg ctgttgaggc gttgggtgaa acaggttgag agagtgtgac
     8821 ttgctgttgg cgcggtgaag ttgatgacgg ctcttttgat agcagtgatc gtctgcacgt
     8881 gtgcctctca tgcggctttg gcgtcggaat gggatccgcc ggagataacc gggagtgccg
     8941 agtggcagat tgactatttg ccaggagaat aaaaagttgc ggcaaacgga ctcagtctca
     9001 aagtcaacct tgatgcctac ttcattgact c
//
LOCUS       DAOSAI010000051         9003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5065_length_9003_cov_3.900201, whole genome shotgun sequence.
ACCESSION   DAOSAI010000051 DAOSAI010000000
VERSION     DAOSAI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5065_length_9003_cov_3.900201"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLM83_03120"
     CDS             complement(<1..717)
                     /locus_tag="PLM83_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70434.1"
                     /translation="MSQPEYASPSLEVWKDLYEAAAQFKRIESWNWLDDSSTFGVQDP
                     VTKTIAYCSVMGALGELYGLAAYVGTEGLLALQKMAFGETDYSDINLGKDMRCLMTTF
                     ESRNDLDKPDIEVIRQLGLSFRGKHNWPRFRSYEPGFFPWYLDAHEAMFVTTILQQAV
                     DVCLRAKDDPDLIPNWDDEVYLVRVAEEKDGELVWEDRMLSPELLPEPELEDSVHIDE
                     LQLMKIRNSAKTTDAVWDADL"
     assembly_gap    1065..1164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1353..2750
                     /locus_tag="PLM83_03125"
     CDS             1353..2750
                     /locus_tag="PLM83_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70435.1"
                     /translation="MTRSKLWRAIMFVLMVLAVSGLAVVAQGGQPPGGGPGTPPEPPG
                     DGFRPGMPGPGGSEIAPPPVGSTYITGTGVYTLSSGSAAMSSETLTASADDQSAIIVS
                     DGAQLNLSGMTIKTSGNTSSMDSSSFYGLNAAILAQAGSRVSITDSTIITSGTGANAV
                     FACGAGASIDMANVDIACEAPGAHGVDATMEGTITMRDVNITTAGNGAAAAIATDRGG
                     GTITASGGTVVTTGTKSPAIYSTGSITVSGATLRSIASEVAVIEGKNSITVNDCVMES
                     RSNFGVFIYQSMSGDASPGTGTFTMTRGSLTAEEGPLFYSTNTDAVIKLSGAVLNCKS
                     GVLLKAGADQWGNVGANGSNVTLIADSQKLKGDIVLDAISTSSVILKNGSLLEGAVNE
                     TGTAKSVSVAMDSSSVWIVTADSKVSSLSDDDTSLANIRSNGHSIYYDKTQEANAWLG
                     GRTVSLQGGGYLAPM"
     gene            complement(3031..4371)
                     /locus_tag="PLM83_03130"
     CDS             complement(3031..4371)
                     /locus_tag="PLM83_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK70436.1"
                     /translation="MKRLDIDHIREIERESNRLLNEKKWAEAEMVLRKALEVTPILVP
                     AINNLAFCLFMQDNPEEALEVLRPNLSADVPNPFASALASQICVALGRMDEAEKHLET
                     AVRSFDAGLPQMRLAPSAISNPWYEYTVMLKRAAGDLGRHQQVIDMYRRHEAYHTTPE
                     DRYLAGVASFNLGRMGRAISYWSKLPWQLGLDFAKVAQSVETGMIPPFSLEYRVPFFD
                     GNTPDMASGIGHMMLSHALLEGGLDNEDRSTAYSMLDLAVKEDRDWGLQLAENILQYP
                     AVSDKVKSAALSILVNHGVCNEGQAVDVVGQDGEPITISLKKITMTCNIAEDEREIIR
                     EAARLVENDGFDEAIAILEEAITNSQEISLYMLLAQAATYTYKGDLDKAYEILSMLRD
                     AAPDDPLVLFRLAEYYCESGNAQEALRCLDQIELEDVSPALQRGIANIRESLYE"
     gene            4978..6219
                     /locus_tag="PLM83_03135"
     CDS             4978..6219
                     /locus_tag="PLM83_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK70437.1"
                     /translation="MANVVERAVQFISRQPRNWKVTACRASIDRCVYQMVFPYLSVYI
                     ALLGATGTQLGAINSAGMVASAVLGLLAGTAVARVGTKKVYSAGVALVALSYLIIGLA
                     QNWVLALAGTMLWWCGSTAAGYGCAIVCGTSLANRDRATAMGCCESLTQGVMGFIGPV
                     IGAMLVAGFGGVSVRGIRPVFFIASIMCLGAWLFVSTQLSETECGIGNGGSNPFLLLR
                     DRPDLVRFVAVSCLTSLPTGMVLPFTQVYASQVKMADQYVLGTMVTAAAVIGLLLGVP
                     LGRLADRMGRKRILFVLAPVYWIGNLLLVWSSRPASLVLVGVFQGAFTMTSMLTQAIA
                     VELAPSERTSDWLAVTKFFKMVAVAAMASVSGLIWDHVGPEYVFLLAVAIDAAVRIPL
                     LLGVPETLELPGRSGESSVRG"
     gene            complement(6491..8023)
                     /locus_tag="PLM83_03140"
     CDS             complement(6491..8023)
                     /locus_tag="PLM83_03140"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOK70438.1"
                     /translation="MPVPNVRSPQDSIAKTAGKVTKRLLTLIVLALLAYHFTGYPAIR
                     RPRPDRTLSPWLAERPIESLLVIAPHPDDETLGCAGVIMYALDQGARVCVALITNGDG
                     SSVPAHTSGKGPLLAPSRYISLGNRRQNETLAALTSLGLEPEDVCFLGYPDGGVSALW
                     SDFWDYSDLFRSQYTKANMSPYSNSFTPNAPYCGLSVVNDICSIIRTFNPSHILLPHP
                     NDTHPDHWGTFALVTYALQRLVCEQELQNPPEMLAYLIHRGDWPVPRLYSPRWPLDPP
                     NTVLRLRAADEWAYQPLTNEDRAQKRQAILEHKSQTAMMRMYMLSFVRSNELFAVLRS
                     PVIAQLPSHQSRSSASRDSHWQTAEPTIWDPQGDTLTRLVSGGADLQAVYAATGNGML
                     YIMAKPKGRPSDNVRYVLDISDFGNPRTQISVSYGPKQSVRTSSSSNLNPDDVQIRRT
                     VDGIEFAIPLTLFQTDRGTGTTAPSAVLLAIHSAMIGDIVVDRTGWTVLRLSEDQPDI
                     FR"
BASE COUNT         1895 a         2259 c         2659 g         2090 t
ORIGIN      
        1 aagatcggca tcccagactg catctgttgt ctttgcagaa ttccggatct tcatcagctg
       61 gagctcatcg atgtgaacgg agtcttccag ctcgggttcg ggcagtagtt cgggcgacaa
      121 catccgatct tcccaaacaa gctcaccatc cttttcctca gccactctta caaggtagac
      181 ttcgtcatcc caattaggga tgagatcggg gtcgtctttg gcccgaaggc agacgtctac
      241 agcttgctgt aagatcgttg ttacgaacat cgcctcgtgc gcatctagat accaagggaa
      301 aaacccaggt tcgtaacttc tgaaccttgg ccagttgtgc ttgccgcgaa acgagaggcc
      361 gagctggcgt atgacttcaa tatcaggctt gtccaaatcg tttcggctct caaaagtggt
      421 catcaggcac ctcatgtctt tgccgagatt aatgtcgctg tagtcggttt cgccaaacgc
      481 catcttctga agcgccagaa gtccctcagt gccaacatac gccgccagac cgtaaagctc
      541 tccgagggca cccatgaccg agcaataagc tatcgtcttt gtgacgggat cttgaactcc
      601 aaaggtggac gagtcgtcga gccaattcca actctcaatg cgcttgaatt gcgcagcggc
      661 ttcgtacaga tccttccaga cctcaagcga aggagacgcg tattccggtt gactcaccca
      721 tattcacctc attccacacg ggcgtgtgtg atattgtact gatatcgaca cgtgattcac
      781 tgggcttttg ctacgggaaa actgccatat ttgcatcgcc tgatgttcgg gacttggcta
      841 ctcgactgtg gttacttagc cggtagaagc atgatcggct caagctggcg gcttgcttct
      901 tcgagcccac atgaaagctt gctctaagtg ggctgctgct agttttccgc attctgcttt
      961 ccagggctca ttttttcgct caagaccagc cgtgtcatca tgtttatcag tgtaccatta
     1021 cgttttgtcc gcggtcaata ggattcacct tcgaggcatc tgcgnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnttatcg cgccttttta gatctatgaa tcaaagcccg
     1201 caggaggtat atgggcccgg gtaccggaac gcctgccccc gttgcggcgt tgaagtaagg
     1261 gggcgttctt gtgaagctgt gcggctaata ctatcgtccg gcggcagcag cggatcggat
     1321 cgtacccatc aatgacgagg agatgtttgc atatgacaag gagtaagctc tggcgggcca
     1381 ttatgttcgt cctgatggta ctggcagtgt cgggcttggc ggtggtcgcg caggggggcc
     1441 agccgcctgg cggggggccg ggcacacccc cggaaccgcc cggtgacgga tttcggccgg
     1501 gcatgcccgg gccaggcgga agcgagattg ccccaccgcc cgtcggcagc acgtacatca
     1561 ctggcacagg ggtctacacg ctaagcagcg gatcggcggc gatgtccagt gagacgctga
     1621 ccgcctccgc agacgaccag tcggcgataa tcgtgtcgga cggagcacag ctgaatctct
     1681 cgggcatgac gatcaaaacc tccggcaaca cctcgtccat ggacagcagc agcttttacg
     1741 gcctcaacgc ggcgattctg gcccaggcgg gtagcagggt atcaataacc gactcgacca
     1801 tcattacctc cggaaccggt gccaatgccg tcttcgcgtg cggtgccggg gcttcgatag
     1861 acatggccaa cgtggacatt gcatgcgagg cgcccggagc ccatggtgtg gatgcgacca
     1921 tggaaggcac catcaccatg agggatgtca atatcactac ggcagggaac ggcgcggccg
     1981 cagcgatcgc cacagacagg ggcggaggaa cgatcaccgc ctcaggcggg acagtagtca
     2041 ccacgggcac caagtcgccg gccatctact ctacgggcag catcactgtg agcggagcga
     2101 cactgaggtc gatagcttcc gaagtggctg tgattgaagg aaagaactcc atcacagtga
     2161 acgactgcgt catggagagc aggagcaact tcggggtgtt catctaccag agcatgtcgg
     2221 gtgacgcaag cccgggcacg ggcacgttca ccatgacccg tggctcgttg actgctgaag
     2281 agggtccgtt gttctactcg accaataccg acgctgtcat caagctgagc ggcgccgtac
     2341 tcaactgcaa gtcgggcgtt ctcctcaagg ccggtgctga ccaatggggt aatgtcgggg
     2401 ccaacggatc caacgtcaca ctcatcgccg actcacagaa actcaaggga gacatcgtgc
     2461 tggacgccat cagcaccagc tcagtcattc tcaagaacgg ctcgctgctg gaaggtgcgg
     2521 tgaatgaaac ggggactgcg aaatccgtat cggtggccat ggacagctcg agcgtgtgga
     2581 ttgtaacggc cgactcgaaa gtgtcctcgc tgagcgacga tgatacttcg ctggcgaata
     2641 tccgaagcaa tggccattca atctactatg acaagacaca ggaggccaat gcctggctcg
     2701 gcggcagaac cgtcagtctt cagggtgggg gatacctggc tcccatgtag gccatgccga
     2761 agtggccgta tggatctatc tggggaggga cgatggtgtc cttccccggt tcgttgttgc
     2821 gcggaggcga cgatttgagg aacgccatgc cggagcaaaa gtccatagtt cagcggtcta
     2881 aatgtgcatg ggctaaccag catgccgggg gccggatttc agggccctat cggtatccat
     2941 cgtggactgc ttctccccag gaatggattt gccctcgggc tccttagccc gcttttgggg
     3001 catgttcagg cctcagtttc aactcacgat ttattcgtag agagattccc gaatgttggc
     3061 gattcctcgc tgcaatgccg gggacacgtc ttcgagctca atctggtcca ggcaacgaag
     3121 tgcttcttgt gcattcccag attcacagta gtactcagcc aggcggaata gaaccaaggg
     3181 gtcatcaggc gccgcatcac gaagcatgct aaggatttca taggctttat caagatcgcc
     3241 cttataagta tacgtagctg cctgggcaag aagcatgtaa agactgattt cctgagaatt
     3301 cgttattgct tcttccaaga tagctatggc ctcatcgaac ccgtcgttct caacgagcct
     3361 ggctgcctcg cggataatct cccgttcgtc ttcggcgata ttacaggtca tggttatctt
     3421 tttcaatgat atggtgattg gttccccatc ttgacccact acgtctacgg cttgaccttc
     3481 gttgcaaacg ccgtggttta ccaggatgct aagggcagcc gatttcacct tatcggatac
     3541 tgctggatac tggaggatgt tctctgcaag ctgcagtccc caatcccggt cctctttcac
     3601 agccagatcg agcatagagt aagcagtgct tcgatcctca ttgtccaacc ctccttcgag
     3661 cagagcgtgg gagagcatca tatggccaat gcctgaagcc atatcggggg tattgccatc
     3721 gaaaaaaggc acacgatact cgagcgaaaa tgggggaatc atccccgtct ctactgattg
     3781 ggctaccttg gcaaagtcga gccccaattg ccaggggagc ttgctccagt acgaaatggc
     3841 tctgcccatt cgaccaaggt tgaatgaagc aacgcctgca agatagcgat cttccggtgt
     3901 tgtgtgatag gcttcgtgac gacggtacat gtcgatcacc tgttgatgtc gaccgagatc
     3961 gccagctgct cttttgagca tgactgtata ctcataccag ggatttgaaa tcgcactcgg
     4021 ggccagacgc atttgcggaa gacccgcatc aaaactgcgg acagcggttt ccaggtgttt
     4081 ttcggcttca tccatgcgcc caagcgccac gcatatttgg gacgcgagcg cgctagcgaa
     4141 ggggttaggg acgtcagccg aaagatttgg tcggagtact tcaagtgctt cttcggggtt
     4201 gtcctgcatg aagaggcaaa aagccagatt gtttatggcg ggcaccagta ttggcgtcac
     4261 ttccagcgcc ttcctgagca ccatctcggc ctcagcccat ttcttctcgt tcagcaggcg
     4321 attgctttct cgttcgatct ctcgaatgtg atctatatct agtcttttca cgacatcgct
     4381 ccgtttcatc agtgaggttc gccaatgcag taaaggcact gtggcataca gcctatgttc
     4441 ataagtctac ttgatattct gccccgctgc caatctggtt tcaaacaatt cgctttccaa
     4501 cggtgggccc aagcttgccc ggctgagaga aatcaatccc tacatgcagg caggggaagg
     4561 gccgattttc cagtctgaga gtgccagtgc tcaatacgtt gctcgtggat ataacactca
     4621 tagactagac aggcacagtg aggttgtttt cccgtaataa gggccggaca ttgtcattgc
     4681 cctgccagcg tacacgcggt aaagaagctg ttggctagtt tgaggaatgg ggaaaaggat
     4741 gctgaaaagt tctggcttga aggcgagaag aggttgattg aagaataatg aggctgtcga
     4801 ctgaacgtgg gggacataga tgcggaaatt gcttatttct tgttaggcga aatgcaatac
     4861 tcagactgga cgtgcggttt tccatgtcat gacgagttgt gccctcttgt gggagatcgg
     4921 cgcggcaggg aagagaagtg tctgtgatgg gccatgggcc ggtgagggga tgaatgggtg
     4981 gctaatgtgg ttgagcgggc tgtgcagttc atatcgaggc agcctcggaa ctggaaagtt
     5041 acagcatgca gggcatcgat tgacaggtgc gtgtaccaga tggtcttccc gtatctcagt
     5101 gtctacatcg cgttgctagg ggccacaggc acccagttgg gcgcgatcaa cagcgcaggg
     5161 atggtcgcaa gcgcagtgct cggtcttttg gctggaaccg ccgtggcacg agtcggcacc
     5221 aagaaagtgt attctgccgg cgtggcgctg gtagcccttt cgtatctgat aatagggttg
     5281 gcccaaaact gggtattggc cctggcaggc actatgctct ggtggtgcgg atctaccgca
     5341 gcaggatatg gctgcgcaat cgtgtgcggg acttcgctgg caaacaggga ccgggcaact
     5401 gcgatgggct gttgcgagag ccttacacag ggagtgatgg gtttcatcgg cccagtaatc
     5461 ggagccatgc ttgttgccgg gttcggaggg gtgagcgtga ggggaatcag gccggtgttc
     5521 ttcatcgctt ccatcatgtg cctcggggct tggctttttg tgtctaccca gctgtcggag
     5581 acggaatgcg gcatcggcaa tggaggcagc aatccttttt tgctgcttcg ggataggcca
     5641 gacctagtgc gttttgttgc cgtgtcatgc ctcaccagct tgccaacggg aatggtgctt
     5701 ccattcaccc aggtgtacgc gagccaggtg aagatggcgg atcagtatgt gctcggaacg
     5761 atggtgaccg cagctgcggt gataggcttg ttgcttggag ttcctctggg gaggctcgcg
     5821 gacaggatgg gccgcaagag aatactcttc gtgttggcac cggtctactg gatcggcaat
     5881 ctgttgcttg tgtggtcatc ccgtccggcc tctctcgtgc ttgtaggcgt gttccagggt
     5941 gcgtttacca tgacaagcat gcttacgcaa gcaatagctg ttgaactggc gccatccgaa
     6001 aggaccagcg attggctggc tgtgacgaag ttctttaaaa tggtcgccgt ggccgccatg
     6061 gcttctgttt ctgggcttat atgggatcac gtgggccctg agtacgtatt cctattggca
     6121 gtcgcaatcg atgcagccgt ccgaatcccg ctcttgctgg gcgtgcccga aacgctcgaa
     6181 ctgccgggcc ggtctgggga aagcagtgtg aggggatgat tccctgggct gttcgacatg
     6241 gattgaggtc aacttactca cgccctaacc aggtctcgat aagtctctgc gcgagcttcg
     6301 agtctgtgct gcctgcagtt tgagcacctg attaaggaat gcttcaaaaa gtccatgcaa
     6361 ttgctctttg gatatgctca tctgatatgt agccattggt tctcctccaa ttccaataga
     6421 gaactagtgg cctttccata cccactacaa cagggacacg gcctttttac agcatcatat
     6481 cggacgtgac tcatcggaat atgtcgggtt ggtcctctga gagtctcagg actgtccagc
     6541 ccgttctgtc tacgacgata tctccaatca tcgcggagtg tatggccagg aggacggcgc
     6601 tgggggcggt tgtgcctgtt cctctgtcgg tctgaaagag tgtgagtgga atcgcgaact
     6661 caatgccgtc gacagtgcgc cgaatttgca cgtcgtcggg gttgagattg ctcgagctgg
     6721 aggttcgcac agactgcttt ggcccgtatg atactgagat ctgtgtgcgg gggttgccga
     6781 agtcggatat gtcgagaacg tatcggacgt tgtctgaagg gcgtcctttg ggttttgcca
     6841 tgatgtatag catgccgttg ccggtggccg catatacggc ctgaagatct gcgcctccgc
     6901 ttacgagacg cgttagggta tcgccttgtg gatcccagat ggtgggttcg gcggtctgcc
     6961 agtgcgagtc gcgggaagca gaggatctgc tttggtgcga tgggagctgt gctatcacag
     7021 gagagcgtag gacggcaaag agttcgttgc tccgcacgaa gctcagcatg tacatacgca
     7081 tcatagcggt ttggcttttg tgttcgagga tggcttgacg tttctgcgcg cggtcttcat
     7141 tggtcagcgg ctggtatgcc cattcatcag cagcgcgcaa acgcaagact gtgttggggg
     7201 gatctagggg ccagcgcggg ctatacagcc gcgggactgg ccagtctccg cggtggatta
     7261 agtacgcaag catttcagga ggattttgga gctcctgctc gcatacgagc ctttgcaatg
     7321 cgtaggtgac cagggcgaag gtcccccaat ggtctgggtg ggtgtcgttg gggtggggga
     7381 ggagtatatg agatggattg aatgttctga tgatgctgca tatatcgttg acgacagaaa
     7441 gtccgcagta aggggcgttc ggggtgaagc tgttggagta aggactcatg ttcgccttgg
     7501 tgtactgaga tctaaatagg tcagaatagt cccagaaatc tgaccatagt gccgacacgc
     7561 cgccatcagg atagcccagg aaacagacgt cctcaggttc aaggccgagt gatgtcagcg
     7621 cggcaagggt ttcattttgc ctccgattgc ccagtgatat gtagcgcgaa ggggcaagga
     7681 gcggtccttt gccgctggtg tgtgcgggca cagaagaccc gtcgccgttg gtgatgagcg
     7741 cgacgcaaac gcgcgcgccc tgatccagag catacatgat gacgcctgca catcccaggg
     7801 tttcgtcgtc agggtggggt gcaattacaa gcaatgattc gatgggccgc tcggccagcc
     7861 acggagagag cgtccggtcg ggccgggggc ggcggattgc ggggtacccg gtgaaatgat
     7921 acgcaagcaa ggccagaaca atgagggtaa gcaggcgctt tgtgactttg cccgcagtct
     7981 tggcgatgct gtcttgggga gagcggacat tgggaacagg cattacaaag atccctccga
     8041 caggccaata tatgcaggcg gagcatggat ggggcgctgc cagctggggc tggcaggagg
     8101 ttccgcttca agcctttatt cacgcccaaa ttgttgaaaa aagctgtggg cgaatgagcc
     8161 ggcatagcgc cagcgattag ggaacccggg acgggagaac cgcatgctgg gtgctgttag
     8221 agggaagttc taaggaaatg gcattaccaa agaagtctta atcagcaggg gcgtacttgc
     8281 attccactca gtactggatc gtgtcccgcc gtgcagcgta gttgatgtat gtttaacgct
     8341 ggccgtggtc tttatgagtt taatgccttt ccgagcgtac aaacacgctt tgaggttgtc
     8401 atgcatcatc tggcatgttc ggagactgta gtgctgtggc tcctccctat catcatgtgc
     8461 agcatgtaat tcataggcca tttacctagg ttggctacat tgctatgcgt gcgcgtgaat
     8521 ttgtccgcga aggcttgctc aacatctagg ggaatttagg atgcagtact ctgttggctt
     8581 gcggaacatc acctgcccaa tcaagttgcg caaggcgacg agctatcccg gggaatccga
     8641 gatatcccgg ccgtttcccg catggaccct attagccaag gttacatgtg cccacgaacc
     8701 ccgatatccg tcggggtcag agggatatca tggtcatgat atagcgcaat atctgtgacc
     8761 tgcggccaag taggttgcaa aaagcatctc agcctgatta ggagggggac aatgtctaag
     8821 tagaccgcaa caaatctctg cgcgatcctc tagtttgtgc tgccgctgtc tggaatttag
     8881 tcggggattg ctaaggtctt tagttgtaga tttggccttc ggcagaatcc gggggaagcg
     8941 ccgtgggacg tggagccggc caaggttcgc cgcgggggcg agcaggtttt gcagaccagt
     9001 cta
//
LOCUS       DAOSAI010000052         9002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5066_length_9002_cov_3.885995, whole genome shotgun sequence.
ACCESSION   DAOSAI010000052 DAOSAI010000000
VERSION     DAOSAI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5066_length_9002_cov_3.885995"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLM83_03145"
     CDS             complement(<1..231)
                     /locus_tag="PLM83_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013282579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK70439.1"
                     /translation="MRQICKRFGGTLALDNVDFAVSKGEVHALIGENGAGKSTLIKIL
                     SGIYSADSGTIAVDGETVEIHSPQDAQRLGIAT"
     gene            complement(269..1231)
                     /locus_tag="PLM83_03150"
     CDS             complement(269..1231)
                     /locus_tag="PLM83_03150"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOK70440.1"
                     /translation="MTRHTKKTAPTITVVGSFTVDMMMRIPRLPVYGETLLGSSFLQA
                     PGGKGANQAVACARLGANVNYVGVLGMDSMAEAALELFRQEGIGTECLFRTDEAATGV
                     GFVLQFPDGNNVCLTDMAANQHLSADMVDRAASLIEESDAVLTCLEIPTDAALRSMEL
                     ARKHGVCSILNPAPAIAGIEKLKGHVDVLTPNHVEAAALLNIDPDAATSADAVAIART
                     LREEGFPAVIITCGEDGAVIADGCGEMKVLGAKVQAVDTTGAGDTFNAALAVAIAEGK
                     PLAEAVMFANCAGALACMKVGCVAAIPYRDEVEERFRSTYSAMN"
     gene            complement(1257..2147)
                     /locus_tag="PLM83_03155"
     CDS             complement(1257..2147)
                     /locus_tag="PLM83_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOK70441.1"
                     /translation="MQQDFKYPKTAEDVTAEMLAMSPEELEKVSRYPLYIHETRDDLY
                     KWIARLMADELKENNAKGEPTRWVLPIGPKGQYPLLAKICNEERISWKNVWAFHMDEY
                     LDWQGRPISYDSPFSFRRYADEHLYKLIDDELLPPKEQIIFPDVYDIDAFTAKIDEVG
                     GVDSTFAGFGYRGHLAFNESPCNRWQKISADEFAAGKTRVVHLLDDTIIALSQRMLGG
                     HSDPIPQMAITIGMADILRSSKIYLLTDGAGWKQWMLRVFLLTTERDPRYAVTLCHGH
                     NGVIVGVDAASAAPITLGIV"
     gene            complement(2147..2932)
                     /locus_tag="PLM83_03160"
     CDS             complement(2147..2932)
                     /locus_tag="PLM83_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK70442.1"
                     /translation="MTSTEPMKPAGALYIGIASQLKNDIVRGAFPDNRLPTEEELSQR
                     FGVSRNTIREALSMLEMDGLITRRQGVGTFIERDKVHVGSRTIGFTESMRLAGRNPGT
                     LMMSFDWEPADQHMSSMLDMRVGEAMGVLKRVRSLDGVPAYYQIERLPAHVIGEDFNP
                     DAMGESLFEYLNKQRDIEVGGSEFSLRATVPNPVIAGMLGMDIGLPLLMIEDLYRSRE
                     DKPLLWSVNYYRSDLYCFRAVERNGTFEMLTVQEERISHREEY"
     gene            complement(3299..5779)
                     /gene="lon"
                     /locus_tag="PLM83_03165"
     CDS             complement(3299..5779)
                     /gene="lon"
                     /locus_tag="PLM83_03165"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOK70443.1"
                     /translation="MARADKLSHKDRENVAQSLPLLPLRGVVVFPNTTLPLEVGREKS
                     VAALEEAMSCGKLIVLAAQKVAKVNEPSPDDIYTVGTVSAIKQLVKLPDGTIRVVVEG
                     VERAVIHEYVQSDPCYMVQVELMPTVEVCDREIEALARNALREFEQFVKLSKKMPVEI
                     MLAIGGTDEPGRLADDIAAHMPLSVEDKQELLSCFSVRTRLERLAVILRRELEILELE
                     RKIHLRVRKQMEKTQKEYYLREQMRAIQKELGDRDDPSSEAAELRQKIEEANFPPEVL
                     EKAVRELDRLESMPPMAAESVVVRNYLDWLLAVPWGVMTEDRLDIERAEAILDEDHWG
                     LDKVKDRILEFLAVRQLSDELRGPILCLVGPPGVGKTSLGKSVARALNRNFVRFSLGG
                     VRDEAEIRGHRRTYVGAMPGKIVQLIKQAGSMNPVILLDEIDKMSADFRGDPSSALLE
                     VLDPEQNSEFGDHYMEVTVDLSNVLFITTANNLYSIPRPLQDRMEIITLPGYTEEDKV
                     QIATHFLIRKQLSENGLSEENVVIQEDAVREIIRCYTRESGVRSLERQIGAICRKIAR
                     EVVAGAEGPFVVGRADLQEYLGVPQYRYGVADLDSKVGVAVGVAWTETGGDTLDIEVS
                     AMKGSGKLILTGKLGDVMRESAQAGYTYVRSRARELGIPEDFYEKMDVHIHVPEGAIP
                     KDGPSAGITMATALASALSGVPVRNDVAMTGEITLRGRVLPVGGLKEKTLAAHRAGIR
                     TMIIPDENKKDLEELPENVAGDMTFIEVSHMDEVLAIALLSAKDDEKSSFGADYAVGE
                     AGDEQGGTMRDVPLPHVVPNTPQPPAVC"
     gene            complement(6433..8175)
                     /gene="lonB"
                     /locus_tag="PLM83_03170"
     CDS             complement(6433..8175)
                     /gene="lonB"
                     /locus_tag="PLM83_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HOK70444.1"
                     /translation="MSNLGVIGLINLFFGIVIGLYFWNLLKVQQGSKTAVDRDSRREM
                     DKLREMRSLSLTRPLTELTRPKRFEDVVGQKDALLALKAALCGPNPQHVILYGPPGVG
                     KTAAARVVLEEAKKSPLSPFRADAVFIEIDANTARFDDRGIADPLIGSVHDPIYQGAG
                     PLGIAGVPQPKPGAVTKAHGGVLFIDEIGELHPIQMNKLLKVLEDRKVFLESAYYSPD
                     DRNIPMYIHDIFKNGLPADFRLVGATTRPAHDIPPAIRSRCLEIYFKALTPEEVGIIA
                     SQAVDKMNMRVEDGVIQIVTKYAQNGRDAVNMVQLAAGVAITEGRKMVMRSDMEWVVE
                     SGQYSPRPERKVPDAPQIGIVNGLAVWGPGMGSVIQVEASAVPAEPGTGSFIVTGIVE
                     EEEQGDGSKILRRRSMARSSVDNVSTVLRNRHALQVGDYHIHVNFPGGAPVDGPSAGI
                     AMATAVFSAARQIPIDNRVAMTGELSVHGKVKPVGGIVAKVEAAVSAGATRVLIPAEN
                     WNERFADRADVQVIAVEDIDEVLRIAASCNAAETESEAACTPWPEVAAAASCRRGDAP
                     GGTGSMPPTVQITG"
     gene            complement(8314..>9002)
                     /locus_tag="PLM83_03175"
     CDS             complement(8314..>9002)
                     /locus_tag="PLM83_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK70445.1"
                     /translation="SITRDVSGEGVQQALLKILEGTVASVPPQGGRKHPHQEFIQLDT
                     TNILFICGGAFDGLDSIIENRIGRKTLGFGAEVKPKVDKNIGDILRQVMPEDLLKYGL
                     IPEFIGRLPVVVPLDALDEESLVRILTEPRNALVKQYRKLFELDGVELEFTDDSLEAV
                     ARKAMERNTGARGLRSIMEHIMLDIMYDIPTRTDVKKVVITKETVADDGKPTMVLAEK
                     KPARKKEETA"
BASE COUNT         2018 a         2700 c         2180 g         2104 t
ORIGIN      
        1 tgtggctatt ccgagtcgct gtgcatcttg aggggaatgt atctcaaccg tctcgccatc
       61 aacggcaata gttccagagt cagcagagta aatgccactg agtatcttga tgagggtgct
      121 tttaccagcg ccgttttccc cgatgagggc gtgcacctcg cctttgctca ccgcaaagtc
      181 gacgttgtca agggcaagcg tgccgccaaa gcgcttgcag atctgcctca tttccaacac
      241 gtattcatcg gacaatccga acagccccct agttcatcgc agagtaggtc gatctaaacc
      301 gctcctcaac ttcatctctg taaggtatag ctgcaacaca tccaactttc atgcaggcta
      361 atgcgccagc acaattggca aacatcaccg cttcagcaag cggcttccct tcagcgatcg
      421 caactgccaa ggcagcgttg aaggtgtcgc cagcgcctgt agtgtcaaca gcttgcacct
      481 ttgcgcctaa aactttcatt tcaccgcagc cgtcggcgat tacggcacca tcttctccgc
      541 aagtgatgat caccgcaggg aagccttctt ctctaagtgt acgcgctatg gccactgcat
      601 ccgccgaggt cgcagcgtct ggatctatat tcaagagagc ggctgcttcc acatggttgg
      661 gggtaagcac atccacgtgc cccttgagct tctcaattcc cgcaatagcg ggagccggat
      721 tgagtatgct gcacacaccg tgtttccgag ccagctccat tgacctaagg gccgcatcag
      781 taggaatctc caggcacgta agaactgcgt cgctttcttc aatcaatgat gcggcccggt
      841 caaccatatc agcactgaga tgctgattgg ctgccatgtc agtgaggcat acattgtttc
      901 cgtcaggaaa ctggagcacg aagccgacac cggtcgccgc ctcgtcagtg cgaaataggc
      961 attcggtgcc gatgccctcc tgtctgaaca gttccaatgc tgcctcagcc attgagtcca
     1021 tgccaagcac gcctacatag ttgacgttgg caccaagacg cgcgcaggcc actgcctggt
     1081 tagcgccttt cccgcctggc gcctgcaaaa agctggagcc aagaagggtt tctccataca
     1141 caggcaatct gggtattctc atcatcatgt ctacagtaaa gctgccaacc acggtaatcg
     1201 tgggcgcagt tttctttgtg tgcctggtca tgatcaatat ctcctcaatg gcaaaatcac
     1261 acgataccca gggtaatcgg ggctgcgctt gcagcatcta ctcccacgat aaccccatta
     1321 tgcccgtggc acagggttac tgcgtagcgt ggatcgcgtt cggtagtaag caggaacacg
     1381 cgcaacatcc actgcttcca tccggcccca tcagtaagca gatatatctt gctggatctc
     1441 agaatatcgg ccatcccgat agtgatcgcc atctgcggaa tgggatccga atgtccgccc
     1501 aacatgcgct gggaaagcgc gatgatagtg tcatcgagca gatgaaccac acgggttttc
     1561 ccagcggcga attcgtctgc agaaatcttc tgccatctgt tacagggcga ctcgttaaag
     1621 gcaagatgtc cgcgataacc gaagccggca aaggtgctgt ctacaccgcc cacctcatca
     1681 atcttggcgg taaaggcgtc tatatcgtaa acgtcaggaa atatgatctg ttcttttgga
     1741 gggagcaatt catcatcaat cagcttgtac aagtgttcat cagcatagcg cctaaaacta
     1801 aagggactat cgtaagaaat gggacggccc tgccaatcga ggtactcgtc catgtggaat
     1861 gcccacacgt tcttccaact gatacgctcc tcgttgcaga tcttggcaag caaggggtat
     1921 tgacccttag ggcctatagg aagaacccag cgggtgggct cgcctttggc gttattctcc
     1981 ttgagttcat cggccatcaa cctagcaatc cacttgtaaa gatcatcgcg agtctcgtga
     2041 atgtacagcg gatagcgtga aaccttctcg agctcctcag gcgacatggc tagcatctct
     2101 gctgttacat cttctgccgt cttcggatac ttgaaatcct gctgcatcag tattcctccc
     2161 tgtgtgatat cctctcttcc tgcaccgtaa gcatctcgaa cgttccatta cgttcaacag
     2221 cgcggaagca gtataaatcg cttctgtagt agttcaccga ccacagcagc ggcttatctt
     2281 ctctagaacg gtaaagatct tctatcatca aaagaggaag tccaatgtcc attccaagca
     2341 ttccggcgat caccggattg ggaacagttg cgcgaaggct gaattcagac ccgccaacct
     2401 ctatgtctct ctgcttattc aagtattcaa atagtgattc gcccatggcg tcaggattga
     2461 agtcttcgcc tatcacgtgc gccggcaatc tctctatctg gtaataagca ggcactccgt
     2521 ctagcgatct caccctctta agcactccca tggcctctcc gaccctcata tctaacatgc
     2581 tggacatatg ctgatcagca ggctcccaat cgaaggacat catcaaggtg cctggattgc
     2641 gtcccgcaag tctcatgctc tcggtaaagc caatagtcct ggagcccaca tggactttat
     2701 cacgctctat aaaggtcccc acaccctgtc gcctagtaat gaggccatcc atctccaaca
     2761 tgctaagagc ctcgcgaatg gtgtttcggc tcacaccgaa gcgctgtgat agctcctcct
     2821 ctgtaggcaa gcgattgtcg gggaaggccc cgcgaactat atcatttttg agttgggacg
     2881 ctatgcctat gtaaagagcg ccggcaggtt tcataggctc agtagatgtc atgagtcatt
     2941 caacctctct tcattacttg ctcgacatag ttgttcaaca actcaggaaa agcatacact
     3001 caatttgtcc caatgtcaac agtgcaaaaa tggtttatac tgcgtgttgt cggcatatca
     3061 gcacctccta tcttgttgga ccattgttcc tgcccgatta cgcaccgcaa tgcacacgta
     3121 aaattgccct tccatgcagc ataaggcctg ccttttgtat gcacatcgag cgccttcctt
     3181 gttggtaata gcaactgcgg acgctatata cgtcctacaa taagcccagg gcgccccgtc
     3241 ggggcgccct agttcgcatc gtatcattaa tctaatctat gttgggcctt cgcccaattc
     3301 agcacacagc cggcggttga ggcgtattgg gaacaacgtg aggcaatggc acgtctctca
     3361 tggttccacc ctgctcgtcc cctgcttcgc ccactgcgta gtcggcgccg aatgatgact
     3421 tttcatcgtc cttagcagag agaagggcga ttgccagcac ctcatccata tgggagactt
     3481 caatgaatgt catgtcgcca gcaacatttt ccggcaactc ctcgagatcc ttcttgttct
     3541 cgtcagggat tatcatcgta cgaatgcctg ctctatgggc agctagtgtc ttctccttaa
     3601 gcccgcccac tggcaatact ctcccacgca gcgttatctc gcccgtcatg gccacgtcat
     3661 ttctcacagg gacgcccgag agagccgagg ccaaagcagt ggccatggtt atgcctgcag
     3721 acggcccgtc tttgggaatt gcaccttccg gaacatggat atggacgtcc atcttctcgt
     3781 agaaatcctc tgggatgcca agttcacgag cgcgcgacct gacataagta tatccggcct
     3841 gggctgactc acgcataacg tcgcccagtt ttccggtgag gatcagctta ccgctgccct
     3901 tcatggccga aacttcgatg tccagggtgt cgccgccagt ctcagtccag gcgacgccta
     3961 ccgccacgcc aaccttgctg tcaaggtcgg caacgccgta acgatactga ggcacaccga
     4021 gatactcctg aagatctgcg cggccaacga cgaaaggccc ttctgccccc gccactactt
     4081 ctctggcaat cttgcggcaa atcgctccta tctggcgttc gagacttcgc acgccagatt
     4141 cccgcgtata acagcggatg atctcgcgca ccgcatcttc ctggataacg acattctctt
     4201 ccgaaagacc gttctccgac aactgcttgc ggatgaggaa atgcgttgca atctgcacct
     4261 tgtcctcttc cgtatagccc ggaagcgtta taatctccat cctatcctga agagggcggg
     4321 ggatggaata caggttgttg gcggtcgtga tgaaaagcac gttggacaaa tccaccgtga
     4381 cctccatgta gtggtcgcca aactccgagt tttgctcggg atcgagcacc tccagcaagg
     4441 cagatgacgg atctccgcga aaatcggcag acatcttgtc gatctcgtcg aggagaatca
     4501 ccgggttcat gctgcccgct tgcttgataa gctgaactat tttgcccggc atcgccccta
     4561 cataagtgcg cctatggcca cgtatctctg cctcatccct gaccccgcca agagaaaacc
     4621 tgacgaagtt cctgttgagg gctctggcaa cagacttgcc cagtgaggtt ttgcccacac
     4681 caggcgggcc cacgaggcag agaataggtc cgcgcaactc gtcagagagc tgccgaacag
     4741 cgagaaattc caaaattcta tctttgacct tgtcaaggcc ccaatgatcc tcgtcgagga
     4801 tggcttcagc ccgctcaata tcgagcctat cctctgtcat tacaccccac ggaacagcga
     4861 ggagccagtc gaggtagttt cggaccacga cagactcggc cgccatcgga ggcatactct
     4921 ccagcctatc aagctcgcga acggccttct caagcacttc gggagggaag ttggcctcct
     4981 caatcttctg cctgagctct gcggcttcag agctggggtc gtctcggtca cccaattctt
     5041 tctgtatagc gcgcatttgc tcgcgaaggt aatattcctt ctgggtcttt tccatctgct
     5101 ttctgactcg caaatggatc ttgcgctcga gctccaggat ctcaagttct cgtctgagga
     5161 tcactgcaag cctctcaagc cgtgttcgaa cgctaaagca cgagagaagc tcctgcttgt
     5221 cctcgacact gagcggcata tgagccgcta tatcgtccgc cagcctgccg ggctcgtccg
     5281 ttcccccgat agccagcatt atctctaccg gcatcttctt tgaaagtttg acgaattgct
     5341 caaattcacg caatgcgttg cgggcaagag cctcgatctc gcggtcgcat acttccacag
     5401 tcggcattag ttcgacttga accatgtaac atggatcgga ttgtacatat tcatggataa
     5461 cggcccgctc gacgccttcc acaacaacgc gtatagtgcc atcaggcaac tttactaact
     5521 gctttatcgc tgacactgtg cctaccgtgt agatgtcgtc gggagatggc tcattaacct
     5581 tagcgacctt ctgagcggca aggacaatca gcttcccgca tgacatcgcc tcttcgagcg
     5641 cggccaccga cttctccctt ccaacctcaa gcggaagggt agtattagga aagaccacaa
     5701 ctcccctgag aggaagaagc ggcagactct gtgcaacgtt ttctctatct ttatgtgata
     5761 acttgtccgc tctagccaag ggattaagca cctcctacat gcattctacc tattctcctt
     5821 gcctctttgc attccttcaa gaaaagcgcc tcggtaaatg caatacgtgg cagaactggg
     5881 caagtgaaaa actgcccttt tcatggagat aagcggaata ttcgaaaagg atacaggcga
     5941 gttggagatt agcccactct gggagcatga gagagtaagg attgcaacat atccacccaa
     6001 aaagccgcag gttaacacgc cagctttgtt ttgccgctgg agaaaggatt aggaccgtaa
     6061 tgtaatcata ccatattggg gattagggga gcgatctgcg tcaccataat actccgagac
     6121 tggtgggcaa ccttgatttg caaagttggc gaaacatccc cacccgcgtg gggacaagac
     6181 ttgacttagc tgaaactctt cagctggcca cttggcgtgc caattctatc ccatttgcca
     6241 gcacctagct gttttcagcc tgtccgctca accccgatgc tttcagattt cgcatatact
     6301 ctaccacatg cacaatgcaa aaggctgtca ccgccccagg cccgcctatc gcggacaatc
     6361 gaggcggcga cagtcataac cgcaaagctg ggcatgcccg cccggcaccc atcttgagca
     6421 gcacagacac actcaccctg taatctgcac tgtgggaggc atcgaacccg ttcctcccgg
     6481 cgcatcccct ctgcggcaac tagctgcggc agcaacctcg ggccaaggtg tgcatgccgc
     6541 ttcagattcg gtctcagctg cattgcagga agccgctatt ctcaaaacct catcgatgtc
     6601 ctccactgcg attacctgca cgtctgcacg gtccgcaaac ctctcgttcc agttctccgc
     6661 agggatcagc acacgggtag cccccgcgct cactgcggcc tcaactttgg cgacaatacc
     6721 ccccaccggc ttcaccttcc cgtgcaccga aagctcaccg gtcatcgcaa ccctgttgtc
     6781 gatgggtatc tgtctcgcag cggagaacac ggcagtagcc atggcaattc ccgccgacgg
     6841 gccatctact ggcgcccctc cggggaaatt gacatgtatg tggtagtccc cgacttgcag
     6901 cgcatgccgg tttcgcagga cggtcgacac gttgtctacc gaactccgcg ccatgctgcg
     6961 acggcgtagg atcttcgagc cgtccccctg ttcttcttcc tccacgattc cggtcacaat
     7021 gaaacttccc gtcccaggct ctgcaggcac agcagaggcc tcgacctgga tcaccgatcc
     7081 cattcccggg ccccaaacgg cgagtccgtt gacgatgcct atctgcggag catcgggcac
     7141 cttccgttcc ggccttggcg aatactgccc gctttctacc acccattcca tgtcagaccg
     7201 catcaccatt tttcggccct cagtgatggc gacgccggcg gcgagctgaa ccatattcac
     7261 agcgtccctg ccgttctgcg catacttggt cactatctga ataacaccgt cttccacacg
     7321 catgttcatc ttgtcaacag cttgcgacgc tatgatgccg acttcctcag gggtcaacgc
     7381 cttgaagtat atctccaggc acctcgatcg aatggcggga gggatatcgt gggcagggcg
     7441 agtagttgcc ccaacaagcc gaaagtcagc aggcagcccg ttcttgaata tgtcgtgaat
     7501 gtacatgggg atattgcgat catcagggct gtagtacgct gactccagga agaccttcct
     7561 gtcttccaac accttaagaa gcttgttcat ctgaatcggg tggagttctc cgatctcgtc
     7621 tatgaaaagc acacccccgt gagccttggt tacggcgccc ggcttgggct ggggtacccc
     7681 tgctattccc agaggacccg caccctgata gatagggtcg tgcacggatc cgatcaatgg
     7741 atctgcgatt ccacggtcat cgaaacgggc tgtattggca tcaatttcaa tgaaaacagc
     7801 atcggcccgg aaaggcgaaa gcgggctctt cttggcctcc tcaagcacaa cccgagcagc
     7861 cgccgtcttg ccgacgccgg gaggaccgta gaggatcaca tgttggggat tgggcccgca
     7921 tagcgccgcc tttagggcaa gaagcgcatc cttctgtccg accacgtctt caaatctctt
     7981 gggcctagtc aactccgtaa gcggcctggt aaggctgagg ctccgcattt cgcggagctt
     8041 atccatttca cggcgggaat cgcggtcaac cgcagttttc gatccctgct ggaccttgag
     8101 gagattccaa aagtaaagac cgatcacgat tccgaaaaag aggtttatca acccaatcac
     8161 gccgaggttg ctcaccgaac atccctcctg tccacggtgt agcatgcccc ggccaacaac
     8221 aactatcccg gcggacgccg tgcgccacgc cgggattatc agcaacgtct ataaaggtct
     8281 tgtcaactcg cgcattcccc aggaaaaccg tccctacgcc gtctcttcct tcttgcgcgc
     8341 cggcttcttc tcggccaaca ccatggttgg cttgccgtcg tcagccacgg tctccttggt
     8401 tatgacgacc ttcttcacat ctgtgcgagt aggtatgtcg tacattatgt caagcataat
     8461 gtgctccatg atcgacctca atcctctggc gcctgtattc ctctccatgg ccttgcgagc
     8521 cacggcctcc agcgaatcgt cggtgaactc gagctccacg ccgtccaact cgaacagctt
     8581 cctatactgc ttcaccagcg cattccgcgg ctcagtcagt atccgaacca acgactcctc
     8641 atcaagggcg tcgaggggaa cgaccactgg aagcctgccg atgaactcag ggatcagacc
     8701 atacttcagg agatcctcgg gcataacttg acggaggata tcgccgatgt tcttgtcgac
     8761 ctttggcttt acctcagcgc caaatcccaa agtctttcga ccgatgcggt tctcgattat
     8821 gctatcgaga ccgtcaaatg cgccgccgca tatgaagagg atattcgtcg tgtcaagctg
     8881 gataaactcc tgatgtgggt gctttcgacc cccttgaggg ggaacgctcg ccactgtccc
     8941 ctcaagtatt ttgaggaggg cctgctggac cccttcaccc gacacatccc ttgttatgga
     9001 cg
//
LOCUS       DAOSAI010000053         8888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5144_length_8888_cov_4.605004, whole genome shotgun sequence.
ACCESSION   DAOSAI010000053 DAOSAI010000000
VERSION     DAOSAI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5144_length_8888_cov_4.605004"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..384
                     /locus_tag="PLM83_03180"
     CDS             262..384
                     /locus_tag="PLM83_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70446.1"
                     /translation="MAIGNYLCLCVSMHEPHVKRLKAGHEEAANELVDMYTDGL"
     gene            551..958
                     /locus_tag="PLM83_03185"
                     /pseudo
     CDS             551..958
                     /locus_tag="PLM83_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
     gene            1274..1642
                     /locus_tag="PLM83_03190"
     CDS             1274..1642
                     /locus_tag="PLM83_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70447.1"
                     /translation="MRRSSKCSKMKTGFGRKRMSSPEILIPLRLRANPLGSTARQSTL
                     DALEGADGGAGMGEIKGIVLLDGKPLPGARIIVREMKETTAFSTDCTGLPRHTVGDDG
                     EFCFNLPAGRRYEVGTALGH"
     gene            1698..1985
                     /locus_tag="PLM83_03195"
     CDS             1698..1985
                     /locus_tag="PLM83_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70448.1"
                     /translation="MRFVEPVEILEPGTGFVFHSISYGEHGYDSMDISSMRTADTAFE
                     INSIPEVQFGGYASDTSHLPDRSGFWPSTSTAMSYPPATACILEQALIQKD"
     assembly_gap    2201..2210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2412..3287)
                     /locus_tag="PLM83_03200"
     CDS             complement(2412..3287)
                     /locus_tag="PLM83_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOK70449.1"
                     /translation="MRVHLDCIACTLRQALEAARLATADIDVQERIIRRSLETLADYD
                     LYRTPAELGSAVHELVKECSGNPDPYRNVKKATIEMAHAIYPGLKRFVEEQDDRLLVA
                     LEVSAVGNLLDAGVYGDLRTVDLEETLKEELSRGFAVCDIDFLRADLLGATKVLIIGD
                     NAGETVLDRILISEIKRACGKEDKVNVFYGVRSAPMINDATAEDAVASGLDHDATIVD
                     TGCCTPGIVLRNATPNFLKLYEEADVVISKGQGNYETLSDSAGRTIHFVLKAKCRAVA
                     EDTEVNIGEYVLLRR"
     gene            complement(3478..5082)
                     /gene="murJ"
                     /locus_tag="PLM83_03205"
     CDS             complement(3478..5082)
                     /gene="murJ"
                     /locus_tag="PLM83_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOK70450.1"
                     /translation="MSGDASKTLAKSAGIVALGSVLSKILGFLRESALASEFGASYAT
                     DAYLMAMIIPTLMLFGVGPAVTTTLIPVFTDLEKRKGRDAAFKSVSCIINACVVVAAA
                     VTVAGFVFAEPLVSFVAPGFSGETYELTVDLTRLLFPIAIFTVITHCITGVLHALGRF
                     AVPAMTGLVQNVIIITSILVFGPKYGITAVAVGTALGAASMFLVQLPALFSSGFRYHF
                     ALDWHDQGLRQVGKLIGPIVLGSVAGQAGTVIIRILASRLPTGSITYLNYSQRLVGLP
                     VGVFGTALVTVLYPTFARMHSEGTREDFAKTFRRSVGVVFFTLVPMAVGLAVLATPVV
                     RLAFERRAFTPEATAATAAALVYACVGIPAMSLSDLSSKAFYAMQDTITPVLVNMGAV
                     GVNVALSLALVEPMGHTGLALAGACQPIASFLMLQAVLRYREKKWTRKARTDGVADNY
                     SLAASIAKSVVSVVVMWFAVSYFDAWITEVFPGMGTGRQIVRLAASVGVGAVVYFAMA
                     AMLKSQELSFAFSTAKKRVKERKTAV"
     gene            5541..7364
                     /locus_tag="PLM83_03210"
     CDS             5541..7364
                     /locus_tag="PLM83_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOK70451.1"
                     /translation="MRGIGVDFGTANSMLATFDGKSAEALLADGHGHPSLVWYQKRRQ
                     PRVGTEAKDAYSSLLGDPDHAFIKSVKRRLASDDTVTLATRTISTETATEVIFNFLLK
                     EAKISPEQDDRFVIAIPNSLTRDQRKEIMRAAKAAGFSPSFLVSEPLAALVGYLEREP
                     IDASERRRVVVADWGAGNVEIALVIMTDDCLVEVASCSLDDVAGDDIDALLAEEALKR
                     FMKRNGLSRQKLEEAMPGLAPEEALLSAAEQAKIALSSDSETQIAKAGFCSVDGVDYD
                     IEESFSKAEFEDLIRPIVDRVLQATEEMLSSAGAKPSDIDLCLLIGESTNLPPVVEGF
                     GRLMTPDRIVRPSASSTLIAEGAAIMAYRNCIPRLVSDISLLLADGSEHELIPAGTAM
                     TPKEESEAPEEFICVDPRQGKIVTAVRANPSVLSSVSDICDRSEHLVELEIDYVQTYR
                     VYNLPRLFVEAGFDETCMLRIDFSSSEAKAEEGLEVEAASPRAALFNWSKNDDGKLDS
                     PSADACRVIPSPTTCESISNILPITAGKRRIWASVPVELLIELEPEIRDVRLMEKYLT
                     PIQRDEYLNSLSSIPAYSGHHEPADSACESDGEPESKSSNG"
     gene            complement(7463..>8888)
                     /gene="pepF"
                     /locus_tag="PLM83_03215"
     CDS             complement(7463..>8888)
                     /gene="pepF"
                     /locus_tag="PLM83_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOK70452.1"
                     /translation="PGLETFRRRLELILSDRPYVLSAETETAIAALREVLDAPEMVYE
                     WTKTSDISFEPAVDSEGHEVPVSFATYEEILEVSPDAALRRSAYTSFSRGLARYQNAL
                     AGTFAAEVKKNVALARIRGYESATHMFLHQQEVPVEVYHNLLDVIEEGLAPHMRRYVD
                     LRRRVLGLDKIMYCDIEAPLDPDFMPEISYEEGARLIIDSVAVMGAEYADIIRVALED
                     RWIDRADNIGKSTGAFCTSTYDVHPYILATWGESMRSVFTIAHELGHAVQSVLASSNQ
                     RFVNVEPSLVFIEAPSTLNELLLAEHILESADDFRMRRWVLMQLLGTYYHNFVRHLLE
                     AELVKRIYAHSEAGRPITAALLSAEKGDILECYWDGVVEIDDSAKLTWMRQPHYYAGL
                     YSYTYSAGLTCATVVAQSVREEGSRAAERWVNVLKQGGSMAPLELMKTAGVDLEDAAT
                     IGDAVNYVGRIVDEVDGGFGGQPH"
BASE COUNT         2199 a         2631 c         2189 g         1859 t
ORIGIN      
        1 ggggggtaat gcccgggtgc gtgttacccc ccctaagaaa cgaccaccat cccctccccc
       61 ctccccgcca caaccccccg cctgctgcaa catgccgcca cgcgcaaaac gcaatgggcc
      121 atggacaatc accacctctg ctctcctaat gccgtaacat actaacattc ctctacaaaa
      181 gcaagaacgc taatataaac atagtggagt gcaacctttt cgcccccgcc catctcttaa
      241 taggtggagg ggggaagagc tgtggccata gggaactatc tatgcctatg cgtctcgatg
      301 cacgaacctc atgtgaagag gcttaaggca ggccatgagg aagcggcgaa cgagttggtc
      361 gacatgtaca ccgacgggct gtgactctca tcctgggcga tcacttccgt ggcgaatcca
      421 cactctactc atggatgtat acaatcctca taaggcactg ccgccattat cagcgtcacc
      481 gacggaaagc ccaaagcacc tccaagctct tgcctcctga ggatttttca cacattcccg
      541 agccgtcgaa ggccttccgc cccgataccg ggaagtcatt gtgatgttct actacgaaga
      601 attttccatc cctgaaatat cggatatcac aggcaagcct gtcggcacaa ttaagagcct
      661 cttgcacaca ggcaccgcct gcagtacccg gcgcgaccgc ccttcaaaag cccaatcaca
      721 agtcaacatc taccacgtgg tggacggggc ctacttgctc aagatagccg agcgtgcgcg
      781 cgaaggcaat gatccggtca aggcacttgc ctactatgac aagttcatct ccgagtatct
      841 ggacaatgag aaaataccgg atgcctcata ctggtcagcc tctatcctgc cggacgaccg
      901 aagcttcata gctatcatct tcccgtgcag atgtggacaa cgcggagcca taatgtgatc
      961 gacgagattc ccgaaggaac gttgaccaaa taggagaggc tgctcaagat ctgcaccgac
     1021 catcccgatc actggtcggc ccctgacgcc acgcatcagc tggcggtttg taccattcag
     1081 gtgatccgag agctaaggaa gaaacatagg caaccgcatt gaagccgcct ttctcctcgt
     1141 cgacatgtac gtcgcggaag gccggtttga cgatgcagag gccatgccgg attaccaatg
     1201 cccacagccc gccgcgaaga gaggggcgat cttctggttg caatgggaga ctatcaaagg
     1261 gcaagagcag cctatgagaa gatcctcgaa atgttcgaag atgaagaccg gcttcggcag
     1321 gaaacgtatg agcagcccgg agatcctgat tccacttcgg ttaagagcaa acccattggg
     1381 atctacggcg aggcagtcaa ctctcgatgc cctagagggc gcagacggcg gtgcaggcat
     1441 gggagagatt aaaggcatcg tgctccttga cgggaaaccc ttgccgggtg cgcgcatcat
     1501 tgtgcgcgaa atgaaggaga ctactgcttt ctccacagat tgcaccggcc tcccgcggca
     1561 caccgtcggc gatgatggcg agttctgttt caacctgccc gcaggacgcc gatacgaggt
     1621 gggaactgcc ctaggtcatt gaggggcagg tcggcgctga actcgccctt gaatcaggga
     1681 aaacaagaga agtcgccttg cgtttcgtag aaccggtaga aatcttggag ccggggacag
     1741 gcttcgtctt tcattcgatc agctacggcg aacacggcta cgattccatg gacataagtt
     1801 cgatgaggac tgctgatacc gcctttgaga tcaacagcat tcccgaagtt cagttcggcg
     1861 ggtacgcctc cgatacttcc catctacctg acaggagcgg attctggccc tcgacgagca
     1921 cggcaatgtc atatcctcca gcaacggcct gtattttgga gcaggcccta atccaaaagg
     1981 actaatagaa gttgagccgg gagaactccg ggaattggag atcttgtccc gcgtctattt
     2041 cacgggcacg tactacacaa ccgaggatca gtccatcgag aagcggagca gagagattct
     2101 tgaggccctc gagccaagcc gagagcacct cagttgtatg caccaacctg aacgacaggg
     2161 agggcgccgc cagcgcctat aaaacgatgc tggcatgcgg nnnnnnnnnn ccttaatacg
     2221 ctggcctact aagccctcac ggtcgaggat gactacgcag agcgagcgaa aggcagagag
     2281 cgcctgttgt gaatggaata cgtgtgattc atgtggcaga ggccgatgct aaaagcatcg
     2341 gcctctcaga ttaacagtcc caaactgccc gcaaactcag gtggaaagca acaggtcaaa
     2401 aatagaattg ctcaccgccg aagcagcaca tactcgccta tgtttacctc ggtatcctcg
     2461 gccactgccc tgcacttcgc cttcagcacg aagtgaatag tgcgccctgc ggaatcgctg
     2521 agcgtctcat agttcccctg acccttactg atcacaacat ccgcttcctc atacagcttc
     2581 aagaagttcg gcgtggcatt tctaagaacg attccaggtg tgcagcagcc agtatccaca
     2641 atagttgcat catgatccag ccctgatgcg acagcatctt ccgctgttgc atcatttatc
     2701 ataggggcgc tgcggactcc atagaacaca ttcaccttat cctcttttcc gcaagccctt
     2761 ttgatctcag atatcagaat ccgatcgagc actgtttcac ctgcattgtc cccaatgatc
     2821 agcaccttgg ttgcgccaag caaatcggcg cgaagaaagt caatatcaca cacggcaaag
     2881 cccctgctca gttcctcttt gagcgtctct tccagatcaa cagtgcgcag atccccatat
     2941 acccccgcat ccaacagatt ccccaccgcc gaaacttcca atgccacgag caatctgtcg
     3001 tcctgctcct caacaaacct tttcagccca gggtaaatgg catgggccat ttcgatggta
     3061 gctttcttga cattccggta aggatccggg ttgcccgaac actccttgac aagctcatga
     3121 actgcactgc ccagctcggc cggggtccta tataggtcat aatccgcaag tgtctcaagg
     3181 gatctgcgga ttatcctttc ctgaacatcg atatcagcgg tagcgaggcg ggccgcctct
     3241 agagcctgcc tcaaagtaca agcaatacaa tctagatgaa ctcgcatatc cgacctccgt
     3301 caattgcccc aaatcagctg cgcctagcat ctgcagaccg agtcgcgcca tgggcaaaaa
     3361 gcgcagccca tgggtacgac tgatgcactc aacacaacat aatacactgg attaataggt
     3421 ccctccgggc aatatccgac tggcaaaagc catagccagc atcaagatta cccctgccta
     3481 cactgctgtt ttcctctcct tgaccctctt ttttgcggtg ctgaaggcaa atgagagttc
     3541 ctggctttta agcatcgcgg ccatcgcaaa gtagacaact gctccaacgc cgactgacgc
     3601 agccagccgc actatctgcc gccctgtccc catacccgga aacacctctg ttatccaagc
     3661 atcaaaatac gatacagcaa accacatcac aacaacagac acaacggact tggcgattga
     3721 cgcggccagg ctgtaattgt cagcgacacc atctgttcta gccttcctcg tccatttctt
     3781 ctcgcgatat ctcaaaacag cttgaagcat caaaaaggaa gctatcggct ggcatgcgcc
     3841 ggccaaagcc aatcccgtat ggcccatagg ctctacaagc gccaaactga gtgccacatt
     3901 gaccccaaca gcccccatat tgaccagcac aggcgtgatt gtgtcttgca tggcataaaa
     3961 agccttcgat gacagatcag acaggctcat agccggtatc cccacgcaag catagaccag
     4021 tgctgcagct gtggcggcag tggcctcagg cgtgaacgcc cgccgttcga aagccaggcg
     4081 gaccacgggg gttgcaagga ccgcaagccc gaccgccatg ggcacaagcg taaagaatac
     4141 tacgcccaca gatcggcgga atgtcttcgc aaagtcctct ctagtcccct cagaatgcat
     4201 ccgcgcgaaa gtcgggtaca agaccgtaac cagcgcagtc ccgaaaacgc ctacaggcaa
     4261 gcccacaagg cgctgagagt aattgagata cgtaatagac cctgtaggga gcctcgatgc
     4321 caatatccgt ataatcaccg tgccagcttg tccggcaaca gagccgagga cgatcgggcc
     4381 tatcaacttg cccacctggc gaagcccttg atcatgccag tcgagcgcga aatgatatct
     4441 gaacccagat gagaataacg ctggcaactg caccagaaac atcgatgcag ccccgagcgc
     4501 cgttccaaca gccaccgccg ttatcccata cttcggtcca aacaccaata tcgaagtgat
     4561 tattatcaca ttctgcacca ggccggtcat tgccggcacc gcaaacctgc ccagtgcgtg
     4621 cagcaccccg gttatgcagt gggtgataac cgtaaagatg gctattggaa acaacagcct
     4681 ggtcaagtcc actgtcagtt cgtaagtctc ccctgaaaat ccaggcgcaa cgaaacttac
     4741 caacggctcg gcaaacacga atccggcaac ggtcacagca gctgccacta cgacgcaggc
     4801 atttatgatg caactgactg acttgaatgc cgcatcgcgc cccttgcgct tctccaagtc
     4861 ggtaaagaca ggtatcaatg tggtggtgac tgcaggtccc acaccaaaca gcatcaacgt
     4921 cggtatgatc atggccatca gatatgcgtc cgtcgcatag ctggcgccga attccgatgc
     4981 gagagcgctc tctcgcagaa agccaagtat cttgctgagt acagagccaa gggccacaat
     5041 ccctgccgac ttggccaaag tcttgctagc atcgcccgac aatactaaca cttcctagcc
     5101 gtgatgatgt atttgagtga catgcaccca taagtctgca gtcgctcccg cagatgtgat
     5161 aacgccgcgc agcgaggcta ggaagcgctt tcgccctccc cgcagagctt cgtcttcgcg
     5221 actgccacta gcaacttcag ctagacaata gtctaccaca tgagaatcac aacaacatcc
     5281 ttcatccatg ttttcctggc acgagaacac tgccagtgcc tgccccgctt catcgattct
     5341 tcacttttac cggccctcaa ctggcgttct taagacccgt cccgcaaatt ccggtcaatt
     5401 ccatccgcta ggacaaaagc cgattatgta ttgctttctt ggaccgggtt tcttatttcg
     5461 gatattgatg gtactatagt aatatacgcc cagaaaccct ttcatgggca gcaaacatgt
     5521 taagaggtgg aactgtgctg atgcgtggta taggagtaga ttttggaaca gctaattcta
     5581 tgttggcaac gtttgatggg aaaagtgctg aagccctgct cgcagacggc cacgggcatc
     5641 cgtcgcttgt ttggtatcaa aagcgcaggc aaccgcgtgt gggaaccgaa gcgaaggacg
     5701 cgtattcgtc ccttctcgga gaccctgacc atgcattcat caaatccgtc aagaggcgtc
     5761 ttgcatcaga cgacaccgtc actctggcaa cgcgcacgat atccacagaa acagcaactg
     5821 aagtgatatt caacttcttg ctgaaagaag cgaaaatctc gcccgagcaa gatgatagat
     5881 ttgtcatcgc aatcccgaac tctctcactc gggatcaacg caaagagatc atgagggcag
     5941 ccaaggctgc aggttttagt cccagcttcc tcgtgtccga gcccctcgct gcgctcgtcg
     6001 gatatctcga acgcgagccc atcgatgcat ccgaacgccg cagagtcgtc gtggcggact
     6061 ggggcgccgg gaatgtggaa atagccttgg tcataatgac cgatgattgc ctcgtcgaag
     6121 tagcaagttg ctccctcgac gatgtagccg gcgatgatat cgatgccctc ctcgcagaag
     6181 aagcgctcaa acgttttatg aagcgcaacg gcctatctcg tcagaaactg gaggaagcga
     6241 tgcccggttt ggctcctgaa gaggcactgc taagcgcggc agaacaggcg aaaatcgctc
     6301 tcagctcgga tagcgagaca cagatcgcaa aggccgggtt ctgcagcgtg gacggggttg
     6361 actacgatat cgaagagtct ttctccaaag ccgagtttga ggatttgatc aggcccattg
     6421 tcgaccgtgt ccttcaggcg acagaagaga tgctctcatc ggcaggcgcc aagccatccg
     6481 acatagatct ctgcctgctc atcggagaaa gcacaaatct gcccccggtt gtagagggct
     6541 ttggacgcct gatgaccccg gatcgcatag tgaggccttc cgcttcctca accctgatcg
     6601 ccgaaggggc agcgatcatg gcctatcgca actgcatacc caggctggtc tctgacatat
     6661 cccttctttt ggctgacggc tcagagcacg aactgatccc tgcgggcaca gcaatgacgc
     6721 ccaaagaaga gtcggaagcg ccagaggagt tcatctgcgt ggatcctagg caggggaaga
     6781 tagtgaccgc tgttcgcgcc aacccttctg tcctgtcatc tgtgtcagac atatgcgaca
     6841 ggtcggaaca cctcgttgaa cttgaaatcg attatgtgca aacttatcgc gtgtacaacc
     6901 tcccccgtct ctttgtggaa gcgggatttg atgaaacttg catgctccga attgactttt
     6961 cgtccagtga ggcgaaggcc gaagaaggac tcgaagttga ggccgcaagc cccagggcag
     7021 cattgttcaa ctggagcaag aatgatgatg gcaaactcga ctcgccttca gcagacgcct
     7081 gcagagtcat cccttcgccg actacatgtg agtctatttc gaatattctc cctattacgg
     7141 caggcaaaag aagaatatgg gcgagtgttc ccgtcgagct cctcatcgag ttagaaccgg
     7201 agattcgcga cgttcgcctc atggaaaaat acctcacccc aattcagcgg gacgagtatc
     7261 tcaattcgct ttcgtctatt ccagcctaca gtggtcacca cgagccagcg gactctgctt
     7321 gcgagtcaga cggcgagccc gaaagcaagt ccagcaatgg ctaatagcca gggagcccgc
     7381 gaccagtttc tgcactgtcg cggcgcttcc ttcttcaata tccgccccct atcccgtcat
     7441 ggccttgacg aagctgagcg gctcagtgag gctgtcctcc aaaacctccg tccacctcgt
     7501 ccacaattcg ccccacatag ttcactgcat cgccaatagt agccgcgtcc tccagatcga
     7561 cgccggccgt tttcatcaat tccaaaggtg ccattgagcc cccttgtttc agcacattca
     7621 cccatctttc agcagctcgc gagccctcct cgcgcacaga ctgcgccaca acagttgcgc
     7681 aggtcagacc tgcagaataa gtgtacgagt aaagaccggc gtaatagtgg ggctgccgca
     7741 tccatgtcag ctttgcacta tcgtctatct ctacaacccc atcccagtag cactccagaa
     7801 tatcgccctt ttccgctgat aacaacgctg ccgttatcgg ccgacccgcc tcggaatgcg
     7861 cgtagatgcg ctttacaagc tccgcttcca acagatggcg gacgaagttg tgataataag
     7921 tccctagaag ctgcataagc acccaacggc gcattcgaaa gtcatcggca ctctccagga
     7981 tgtgctccgc gagaagcagt tcattcaagg tcgatggcgc ttctatgaaa accagggacg
     8041 gttcgacgtt tacgaaccgc tggtttgaac ttgccaaaac cgactgcact gcatggccaa
     8101 gctcatgagc gatggtgaag actgaacgca tgctctcgcc ccatgttgca aggatataag
     8161 ggtgcacgtc gtacgtagac gtacagaaag ccccggtcga cttgccgata ttatccgcac
     8221 gatcgatcca cctgtcctcc agagcgaccc gaatgatatc cgcatactcc gctcccatga
     8281 ccgcaacaga atctatgatg agcctagctc cctcttcata gctgatctca ggcatgaagt
     8341 cagggtcaag gggtgcctcg atatcgcagt acattatctt gtccagcccc agcacgcgcc
     8401 ttcgaaggtc tacataccgt ctcatatgcg gcgcaagacc ttcctcaatc acatcgagca
     8461 gattgtggta tacctccacc ggcacctcct gctgatgcag aaacatatgg gttgccgact
     8521 cgtagcccct gatcctcgcc agcgccacat tcttctttac ctctgctgcg aacgtgccgg
     8581 ccaaggcatt ctgatagcgc gccagaccgc gcgagaaaga agtatacgcg ctgcgcctca
     8641 acgcagcatc gggcgagacc tcgagtatct cttcatacgt tgcaaaggag acaggaacct
     8701 catgtccctc gctgtctacg gcaggttcaa atgagatgtc cgacgtcttt gtccattcgt
     8761 acaccatctc tggagcgtcg agaacctccc taagtgctgc gatggcagtt tcggtttcag
     8821 ctgacagaac atatggcctg tcggagagga ttagctccag acgccttctg aacgtctcaa
     8881 ggcctggt
//
LOCUS       DAOSAI010000054         8842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5167_length_8842_cov_4.154205, whole genome shotgun sequence.
ACCESSION   DAOSAI010000054 DAOSAI010000000
VERSION     DAOSAI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8842
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5167_length_8842_cov_4.154205"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            518..1282
                     /locus_tag="PLM83_03220"
     CDS             518..1282
                     /locus_tag="PLM83_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOK70453.1"
                     /translation="MHSVHSPDSDATMDEMCEAAIDAGLRGICFTEHVDLDPHYCSYG
                     SYDYAETMKDTARCREKYGDRLIIKSGVEITYLTEYADDVRQFLTEHQFDYVLGSIHI
                     IDHVFVGSEEHFIGKTEQEAYEPYWREMIAMMESGLIKRVGHMDYIKNHRPKEYGPFD
                     LKKWEDYIRHILHLVVENGATLEVNTSAIRRGFPEPYPSWDILELYREVGGTQVTIGS
                     DAHKPDDIGQYFADVAKRLDEMGFVVCGEEIFSCRV"
     gene            1396..1929
                     /locus_tag="PLM83_03225"
                     /pseudo
     CDS             1396..1929
                     /locus_tag="PLM83_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
     gene            2510..3679
                     /locus_tag="PLM83_03230"
     CDS             2510..3679
                     /locus_tag="PLM83_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOK70454.1"
                     /translation="MSTSEYGFDYADLRESMVEEFCELVRIDAESGQEAELASVVAAK
                     LENLGFDVTQDDAGSKFGGNAGNVIGRFPGVPGLEPILLSAHLDRVTPGKGIVPSIDG
                     DRIVSGGDTVLAADDLGGVVAILAGIRMARAACKRLPPLEVVFTVSEEKGLRGARCLD
                     YSQIQSKTGYIFDASSPVGVVVVQSPTHISVDATITGRAAHAAVNPEGGISAIRVAAE
                     AIGKFPFGRVDDRTTANIGIISGGTATNIVCEKVSFKGEIRSLDTQRALDLAKEVAEQ
                     IKTTAGGYEASAEVTTTVDYYGYTIPRDAQVVRRVEKALAMSGREAVLDSTMGGSDGN
                     IFNTKGIESVVIGMGAMDVHSTKESLPISELVAAGELARNIILLASGMQDASLAD"
     gene            4770..5756
                     /locus_tag="PLM83_03235"
     CDS             4770..5756
                     /locus_tag="PLM83_03235"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOK70455.1"
                     /translation="MFKARLLCPGLLMLVVIALTIPMSFFEYVPWMEGDMASRVMEPV
                     LVCEEIIYKEIGEMSLGFDIYRSSEISASPHMAPVVVYVHGGAWVVGDKRGGLRLAWP
                     MLEALCSKGFVFVSTDYRLANTYTKFPAPVEDVRDTVRYLKKNTDQLGIDSERIALMG
                     VSAGGHLALMAALAPDDSLGGDPKLCTYSGRVRAVVSWCGPTDLRIEQHRTPIREMLV
                     TGLVGQPFQSGSYLYSAASPAAYVSSTSPPILLLHSEEDSLVPYGQAQCMYEQAMVVG
                     ADVMLISIENSGHVYKKIDDREIQPELEDILKLIADYIERQLGLAFGPCEHR"
     gene            6184..6489
                     /locus_tag="PLM83_03240"
     CDS             6184..6489
                     /locus_tag="PLM83_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOK70456.1"
                     /translation="MACEKQDRRSGQPEAKRVGVAAIIIQDRLNAAPAVNKILSEYGE
                     IIVGRMGIPYTDRHVSVIALIVDGDTDAIGAMTGKLGSIPGVKSRVTLVPSDNESGE"
     gene            6515..7693
                     /locus_tag="PLM83_03245"
     CDS             6515..7693
                     /locus_tag="PLM83_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK70457.1"
                     /translation="MAVSHPEELGLNEVIALLNPPGPAQEMALWQAAHSVRVSVMGMC
                     VHMRAIIEFSNYCRQDCLYCGLRRSNNRISRYRMSPDEIVEAAIEAHSIHPFGTFVLQ
                     SGEDPGYAADQLAVAVRRIAETTGSAVTLSIGQMPDEDYRVLREAGADRFLLKFETSD
                     ERLFRQLKPTTSLEHRLGCLRSLRRMGYQVGSGIILGLPGQDLSSVAADLLLCRDEDY
                     EMVSIGPFIPHPDTPLGNGESGVAGGSPKAGFDTNGTEASTGAAVLASVRTTIDAVAV
                     ARLLVPRAHMPATTALAVMGGNDASWKEIAPSPRLTDARSLALVSGANVLMLDVTPAK
                     YREFYTIYPGKSGADGDVISDFVRQAKEDIDALNMTICPGKGDSPKMPWGRGGKGVDR
                     "
     gene            7683..>8842
                     /gene="hydG"
                     /locus_tag="PLM83_03250"
     CDS             7683..>8842
                     /gene="hydG"
                     /locus_tag="PLM83_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOK70458.1"
                     /translation="MTGRTECTFIDDEKIRGALEEGARYSPAAVRDVLAKARELQGLE
                     LEELAALLWVEDLALEEEIYAVARKIKETIYGKRLVFFAPLYVSDYCVNNCRYCAYKR
                     DNRQPRRRLSMDEVRQEVLAIEDMGHKRIALEAGEDPVNCPVDYITEVMETIYDTHSG
                     NGSIRRINVNIAATTVDDYRKLKDAGIGTYVLFQETYHRDTYVAVHDGPKSDYDWHAT
                     AMDRAIEGGIDDVGIGVLYGLYDWRYETVAMLAHAHHLDETYGVGPHTISFPRLRPAA
                     GVSLENFPSLVSDNDFKKIVACLRLAVPYTGMILSTRESPEFREEVIALGISQISAGS
                     CTGVGEYSRRKAHDTPQFEVNDRRDLDEIIRDLLPSGYVPSFCTACYRSGRTG"
BASE COUNT         2009 a         2195 c         2652 g         1986 t
ORIGIN      
        1 aaacggaatt gctgggcaca tggggcaatt tcggccgtgc gcgttccatc gcacagctga
       61 cagacggcag ccctgttgca gaggcagaac tcatgtttgc actgggagat ttggatggag
      121 gagatctatg agggtcccgt gctattctgc gttctgatgg atgtgccatt tggattttcg
      181 cttacaagat tgatggagag ggcattgcgt agcatttgct gagagccacc gccggttggg
      241 cgagaagagt agagactagc ttgggctcga cgcttgtttg gagtgtgaat tcagattccc
      301 gtgactcgtc gacagagaag ataaggcagt ttcctattgc gttggcctgt tgtacttttc
      361 ggacaggctc tggccgcgca aagatccgca tattcgaaga gagagtgtat agtctgtccc
      421 ctcgaagagg cacccctttc ccgcaaagtc tgtgctatac taaaggctgt gctacagaaa
      481 gagaggtgcc ctattagaag aattgatcga ttgtcatatg cactctgtac attcacccga
      541 ttctgacgcc acgatggacg aaatgtgtga ggctgcgatc gacgcaggac tacgcggaat
      601 atgtttcaca gaacatgtcg atttggaccc gcactattgc tcctatggca gttatgacta
      661 cgctgaaact atgaaagata ccgcgagatg cagagaaaaa tacggtgacc gcctgataat
      721 caagtcggga gtggagatca cctatctgac tgagtacgcc gatgatgtcc gacagttcct
      781 tacggagcac cagttcgatt atgtcctcgg ttcgatacac ataatcgacc acgtcttcgt
      841 aggaagcgaa gaacatttta tcggcaagac tgagcaggaa gcctatgagc cttattggcg
      901 agagatgatt gccatgatgg aaagcggcct catcaagaga gtaggccaca tggattatat
      961 aaagaatcac aggcctaaag agtatggtcc tttcgatttg aaaaaatggg aggattacat
     1021 caggcatata ctccatttgg tcgtggaaaa cggagctact ctcgaggtca atacgtcagc
     1081 cataagaaga ggctttccgg aaccctatcc cagctgggac atactcgagc tctaccggga
     1141 ggtcggggga acgcaggtga cgataggatc ggatgcacac aaacctgacg acattggaca
     1201 gtatttcgcc gatgttgcca aacgcctaga tgaaatgggt tttgtcgttt gtggagaaga
     1261 gatcttctcc tgtagggtct gacaacagca tatgtaccca tagcgccggc gcagactgtg
     1321 ggcggcacgc atatggtgca gtggactcta tccaggggag tgatctcaat gccagatgat
     1381 agggaagagt caggaatgcg ccaggcgccg cagtgccctg gcgggttcct ataccagatc
     1441 agatctggtg ataccctcta cgcgcttgcg ggccgttacg ggacaacagt agaagctata
     1501 acaagggcca acccaggtat agaccctaac agcctccgcg ttgggcagct tatctgcatc
     1561 ccggttgccg cggctccgcc cggaccgcct gcaggggctt gtccagctgg atccacgcct
     1621 tatgtaatcc gctcgggaga cactttctac agtattgctc ggcgctttaa caccaccgtt
     1681 gctgctctta ctgcagccaa cccgcgcgta aacccgaaca acctccgtgt cggccagttg
     1741 gtatgcatcc caactgctcc gccgccaact gcggcttgtc cgccgggttc tacgccctac
     1801 gcaattcgct cgggcgatac gttttacagg attgcccaaa gggcgggaat tagtgtaagc
     1861 gctctagttg cggccaaccc gggcgtcgat cccaatagac tccgcgtggg ccaggtcata
     1921 tgcattccag gcgcaccgcc tcctagacgt gtttcgtgca cgatgaatct tctggcgcca
     1981 gccgggagtc ctgcgcccgg tgccacgggt agattgtgga tagacacgaa cgctgcaggc
     2041 aacttgcaga taacagtggc aggcgtggat ctccctgctc ctgccacctt aggagccaac
     2101 atctacactg cagtattttc cgcggacggg gtgcggtttt ccgttcctat ggtcgctacg
     2161 gtcgagggtc ggtggaccgg gactatggtt cagccgcctt caactgcatt gctgacgcgc
     2221 gggcgcgtgg acatatatcc aggccctgtt ttaagtggcc tattggcaaa ttgcaggtag
     2281 aagcctaaac cagcctgcga tgcgtgagat ggtaagagca tcggcccgcc tctcgccctt
     2341 tgcgccctga gcgcggaggg tgcaggggtg ggccggcgcg ttaaggtcag catgtggacg
     2401 gaggagtcgg tatggcgcga gcagaatcct tcggggcaag cgaagtactg aaagaggtca
     2461 ggccgcgcgt tgaagcgcgg ggatcgaatt tggaaagtgg tgagtctgta tgtcgacttc
     2521 agaatacggc tttgactacg ctgatctgcg cgaatcgatg gtggaggagt tctgtgaact
     2581 cgtgaggatc gacgcggaat caggtcaaga ggcggagcta gccagtgtag tggctgcgaa
     2641 actggagaac ttggggtttg atgtaacgca ggatgatgcc gggagcaagt tcgggggaaa
     2701 cgcaggaaac gttattggac gttttccagg cgtgccaggc cttgagccta ttctgctttc
     2761 cgcccacctt gacagggtga ctccgggcaa gggcattgtg ccgtcaatcg acggcgatcg
     2821 aattgtgtcg gggggagata ctgttctggc ggcagacgac cttggcggag tggttgcgat
     2881 tcttgccggt ataagaatgg cgcgcgcagc atgcaagaga ttaccgccgt tggaagtagt
     2941 atttactgtg agcgaggaaa agggcctcag aggggccaga tgcctcgatt actctcagat
     3001 acaatcgaaa acgggctaca tcttcgatgc ctcgagcccg gtaggcgtgg ttgtcgttca
     3061 aagccctacg cacatcagtg ttgatgcaac gattacgggt agagcagccc atgcagcggt
     3121 aaatcccgaa ggcggcataa gcgcgatacg cgtggctgct gaggctatcg gtaagttccc
     3181 gtttggacga gtcgatgacc gtacgactgc gaacatcggt ataatctcgg gcggaacggc
     3241 cacgaacatc gtctgcgaga aggtctcgtt caaaggagaa atacgcagtt tggatacgca
     3301 gcgcgcactc gatcttgcga aggaggtagc ggagcagata aagactactg ccggaggata
     3361 tgaggcttcg gcggaggtca caactacggt ggactactac gggtacacca taccccgcga
     3421 tgcccaagtg gtgaggcgag tcgaaaaggc gttggctatg tcaggccggg aggctgtttt
     3481 agattcgacc atgggcggca gcgatgggaa catattcaac accaagggca tagagagtgt
     3541 tgtcattggc atgggggcca tggatgttca ttcgacaaag gaaagtctgc ccatttccga
     3601 gctggtggcc gccggcgagc tggccaggaa cattatactg ctggcgtcgg gcatgcagga
     3661 tgcatcttta gccgactgag cagcttttat tggcgctcgg ggcacgtttt tcgggggaaa
     3721 gctcagaagc agcctattga cacgcattgg actcgttttt gtgagttcgg gccggtttat
     3781 gcattgagct tgtccacaaa aaggtatccg ctggttcgcc tcaatgggat gtgtctcgaa
     3841 gttggccagg ttcgttgaca acccccgctt ctcttcgcag cagaccttgg ccatcgccac
     3901 acacttcttg tccgggcctc aaagggcagc cgagcatttc agcgctcttt gcgcgactgt
     3961 tgaatcgcgc tcgccatccg gcgtaagggc ttcagccgct aggatttggt gagtatcgcg
     4021 gctatgttag gcggtgggtg tgcaaggcgg gcaagggaat agccattgca aacactcacc
     4081 gccgttgcct acagatgcag catatatccg cagctcagac cttggttgtt tgatccgggc
     4141 aagatacttc ttccaagctt aggcgctaac catcttagac tgcgatgtgg aatccaggtt
     4201 cttctagaac atgggccttg tcatgccctt gattattgtc aaggagagga ccgcggcgcg
     4261 tatgcaacgg cacgacagat cttgctgcgg tctgccataa caggcacgcc ctgttggaaa
     4321 agtttaagag cagccctgtg ttgcgaagat tcccgccgaa ctgtctgatg atcgcgtcta
     4381 cgatgaatac ggcgcattaa cggccacgcg aaagcagaac atctccttca ttgacctagt
     4441 accctactcc tttcttccat gcatcttcat atatctcggg gcccttcggg cagaatccga
     4501 tcgctcggga tcatggaatt cacctgctca ttgcatgttt taactatact gcacaacctt
     4561 tggctctggt gcattagccc ctctcttcaa ccgctttatc tctgacccgc ccgctatctg
     4621 ccgagtgata acacgaacat gtcaatgaat agatctgata tcggcgtcaa ataacgtggc
     4681 gggcggcatt gttctcgcgg gggctgctga tgaccgtggc ggcgaagctg ttcaattgca
     4741 cgccgcgatc gagagggatg aaaggcataa tgttcaaagc caggctcctc tgtcccggtc
     4801 tgctcatgct ggtagttatc gcccttacaa ttccgatgtc gttcttcgag tatgtgccat
     4861 ggatggaggg ggacatggca tcgcgggtaa tggagccagt attggtctgc gaagagatca
     4921 tctacaaaga gattggcgaa atgtcattgg ggttcgacat ctacagaagc agcgagatat
     4981 cggccagccc tcacatggcg ccggtggtgg tatatgtcca cggcggcgcc tgggtggtcg
     5041 gggacaagcg gggcggactc cggcttgcct ggcccatgtt ggaagcgctc tgcagcaagg
     5101 gtttcgtctt cgtaagcacc gattaccgtc ttgcaaacac ctacaccaaa ttccctgccc
     5161 ctgttgagga cgtaagagac accgtgcgct atctcaagaa aaacaccgat caactcggga
     5221 tcgattccga gaggatagct ctcatgggcg tatcggccgg cggacacctt gcgctcatgg
     5281 cggcgttggc gccggacgat agtctcggag gcgatcccaa gctatgcacg tattccggca
     5341 gggtgcgtgc tgttgtatcg tggtgcggtc cgacagattt gcgtatcgaa cagcatcgca
     5401 cgcctatccg cgaaatgctg gttacagggc ttgtgggtca acccttccag agcggctcgt
     5461 atctgtatag tgcagcgagt ccagcagcgt atgtctccag cacaagtccc ccaattctcc
     5521 ttttgcattc cgaggaggat agccttgtcc cctatgggca ggcgcaatgt atgtacgaac
     5581 aggccatggt tgtgggagcc gatgtgatgc tgatcagtat cgagaattcg ggacatgtat
     5641 acaagaagat agatgacagg gaaatccagc cggagctcga agatatactc aagttgattg
     5701 ccgactacat agaacggcag ctggggttag cattcggccc ctgcgagcat cggtagcttc
     5761 aaacggagca ggcattctgc tgtcagacga aaaggaattc ttcctggcgg cgcgagctgc
     5821 cctgtgatac aatggttttg ggtaaagcca taaggtttgg ctgagctatt gacacaactg
     5881 aataggtatg cctgcgggta gggttaacgc ctcaacctag ggctggtgat ttttgtgtgt
     5941 gcctgtccga agcttttggg gaatgcttaa aacgggttcc tgaagctagg gggcgcgcta
     6001 tccatctgtg atatccgatc ttaagcacca aggatcatta gcccgtcctc gcgacgggtt
     6061 ttttgattgg tgcataccta gcggagtctg gcaacgagcc gctgatcaac gttcccttcc
     6121 cgaaggtatt acctgagaaa acgtcagatc ttatctgaca gttccaaagg aggttcaact
     6181 gaaatggcgt gtgaaaagca agataggaga tccggccagc cagaggccaa gagagtgggt
     6241 gtagccgcca tcataattca ggaccggctg aatgcggcgc ccgctgtaaa caagatcctg
     6301 agcgaatacg gcgagatcat agtgggacgc atgggcattc cctatacaga caggcacgtc
     6361 tctgtgattg ccctcatcgt tgacggcgac acagatgcga tcggggccat gaccgggaaa
     6421 cttggctcga tccccggggt caaaagtcgc gtcacattgg tcccttccga caacgagagt
     6481 ggtgaatgac gcagatttca cagaggtttt ggggatggcc gtgtcccatc ctgaagagct
     6541 tggcctcaac gaggtgatag cccttttgaa ccctccggga cctgctcagg agatggcgct
     6601 gtggcaggca gcccattcgg tcagggtttc cgtgatgggc atgtgtgtac acatgagggc
     6661 gataatcgag ttctcaaatt actgccgcca ggattgcctc tattgcggcc tccgccggag
     6721 caacaatagg atttcgcggt acaggatgag cccggacgag attgtggagg ccgcaataga
     6781 agctcacagc atccacccat tcggcacatt cgtgctgcag tcgggggagg atccgggtta
     6841 tgctgcagat caactagcgg ttgcggttcg aaggattgcg gaaactactg gatccgcggt
     6901 cactctcagc attgggcaga tgcccgatga ggattaccgg gttctcaggg aagcgggggc
     6961 cgaccgattt ctcttaaagt tcgagaccag cgatgaacgg cttttccgac aactgaagcc
     7021 caccacctcg ttagagcatc gccttggatg tctaagatcc ctgaggagga tggggtatca
     7081 agtaggatcc ggcattatcc tcggccttcc tgggcaggat ctctcttctg ttgcggccga
     7141 tctcttgctc tgccgggatg aggattacga aatggtcagc atagggccgt tcatcccgca
     7201 tcccgacact ccgctgggca atggggaaag cggtgtcgcc ggtggcagcc caaaagcggg
     7261 ctttgacacc aacgggacgg aggcgtcgac aggggcggca gtgttagcct cagtgcgaac
     7321 aaccatagat gccgtagctg tggcccgtct tctcgttccg cgtgcccata tgcctgccac
     7381 cacggctctt gcggttatgg gcgggaatga tgcttcgtgg aaagagattg cgccatctcc
     7441 tcgactgaca gatgcccgga gtctggccct tgtatctggt gcaaatgtgt tgatgttgga
     7501 tgtgactcca gcgaagtatc gcgagttcta cacgatctac cctgggaaat ctggagccga
     7561 tggtgatgtg atttcggatt tcgtgaggca ggcaaaagag gatattgacg ctttgaatat
     7621 gacgatttgc cctggcaagg gcgacagtcc gaaaatgcct tggggaaggg gaggtaaagg
     7681 tgttgacagg tagaaccgag tgcaccttca tagacgatga gaagatccgt ggagccctcg
     7741 aggaaggggc aaggtacagt ccagcggcgg tcagagatgt tttggcgaag gcgcgagaat
     7801 tgcagggctt ggaacttgag gagctggcag ccttgctctg ggtcgaagac ctggccctcg
     7861 aagaagagat ttacgccgtt gctagaaaaa tcaaagagac catttatggg aaaagactag
     7921 tttttttcgc ccctctgtat gtgagcgact attgtgtgaa taattgcaga tactgtgcct
     7981 acaagaggga caatcggcag cctaggcgaa gattgagtat ggatgaggtc agacaggagg
     8041 tccttgccat tgaggacatg ggccacaagc gcatcgctct ggaggccggc gaggacccgg
     8101 tcaattgtcc cgtcgattac atcaccgagg tgatggagac catatacgac acgcattcag
     8161 ggaacgggag cattcgccgg ataaatgtga acattgccgc tacaacagtg gatgactacc
     8221 ggaagctgaa ggacgccgga atcgggactt acgtactgtt ccaggagacg taccaccgcg
     8281 atacgtatgt ggctgtccat gatggaccaa agtccgacta tgattggcac gctacggcca
     8341 tggacagggc gatagaaggc ggaatcgatg acgtgggaat aggcgtgctc tacggcttgt
     8401 atgactggcg ctacgagacc gtcgccatgc ttgcccatgc ccaccatctt gatgagacat
     8461 acggggttgg accgcatacc atttcattcc cgcgactgag gcctgcagca ggcgtgtcgc
     8521 tcgagaattt tccaagcctc gtctccgata acgacttcaa gaagatagtg gcgtgcctga
     8581 ggctggctgt gccatataca gggatgatcc tgtcgacaag ggagtcgccg gagttccgcg
     8641 aggaagtgat agctctgggg atttcgcaga taagcgccgg ttcttgcact ggcgtaggcg
     8701 agtatagcag gcgcaaggcg catgatactc cccagttcga ggtgaacgac cgtcgtgatc
     8761 tggatgagat aatcagggac cttttgccgt caggttacgt acccagtttc tgcactgcat
     8821 gttacaggtc gggtaggacg gg
//
LOCUS       DAOSAI010000055         8787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5226_length_8787_cov_3.382501, whole genome shotgun sequence.
ACCESSION   DAOSAI010000055 DAOSAI010000000
VERSION     DAOSAI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5226_length_8787_cov_3.382501"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="PLM83_03255"
     CDS             <1..1619
                     /locus_tag="PLM83_03255"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOK70459.1"
                     /translation="PSVVITAPVGGAELHGTCEVEWTATDPDDDGDNLLIDLAWTRDG
                     VEWHDLGTGLSNTGAFEWNTIEVGKAGDSFRLRARVTDPTGAYHEFVTGEFTIINNAP
                     TADFGFTPSPATRRDVVKFTDKSTDDGRIVAWHWEFGDGTESSEQNPEHRYAEKGEFT
                     IALTVTDNGELKGTVEKSIVVGNAAPIAAFSFSPESPKAGEVVTFTNESTDDAGIIAY
                     FWEFGDDTTSVAPEPTHTYATSGIFTVKLMVIDDDYTASSVEHDVEVVNAAPVAAFSF
                     TPASPTIHDNVEFIDESTDDGPIAAWHWTFGDGTESDQQNPSHKYASKGKYTVTLTVT
                     DNGGLWGTAEGEITVVNLPPEVTIVKPEVGTVWTGMQTIEWEAVDPDDDANTLKITLE
                     YKPAGGQDWQTIAANQANTGTCVWDTSSVKQGGRYRIKVTATDPSDATGQAMSDEFTI
                     VVLTHAIVAAPNPAKDGVTFYYDLAADATLYVYDIAGRLVYSAELPAAAHAHEWNLRS
                     GDRPIANGVYLYIAVSGQEKTEVGRLVISR"
     gene            1718..2596
                     /locus_tag="PLM83_03260"
     CDS             1718..2596
                     /locus_tag="PLM83_03260"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOK70460.1"
                     /translation="MRVRISALAFTLILALTMSASALAADDPVGTAAMMDIGMGARAL
                     GMGGAHIAVADDSAAVYYNPAGLAFMSERHVGSLYTQQYGAAGFMAAAYAQKHVGVGG
                     LRLDASGIEGTDEFANETGVFGVSDFTVIVCGGIEALPNLGIGAALKYYSQALPDNPG
                     KGVTGDIGLLFIAPSKKLRVGAVVKNVGGELKYSSGYADAFDRSVGAGVAFYPVDRLL
                     LAADLIYKDELETRIGGEFQLGRILLRAGGAFGSKQNSLTAGAGFSASMFTIDYAYQT
                     HSILPDSHRLSLGMKF"
     gene            complement(2717..5263)
                     /locus_tag="PLM83_03265"
                     /pseudo
     CDS             complement(2717..5263)
                     /locus_tag="PLM83_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515253.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
     gene            5646..5906
                     /locus_tag="PLM83_03270"
     CDS             5646..5906
                     /locus_tag="PLM83_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOK70461.1"
                     /translation="MDTDHQKDNLCPLRDMPISAEASIVELRATGLARRRLLDLGLVP
                     GTKITPIRRSPFRDPTAYLVRGTIIALRQEDSAQIIVRKSDC"
     gene            5906..6529
                     /locus_tag="PLM83_03275"
     CDS             5906..6529
                     /locus_tag="PLM83_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOK70462.1"
                     /translation="MKKAMPTPHTLSKGLRSLSRPEGDIPIVGLAGNPNTGKSTVFNA
                     LTGLRQHTGNWPGKTVGQASGLLRYSGDTIRLVDLPGTYSLFATSEEERIARDFICSN
                     TASAIVIVVDATCLERNLNLVLQVLEITAKAILCVNLIDEARQKGIRVNREVLSRELG
                     IPVVLTAARSGEGLDELKRTILHSVGRGEPVAANHQTSVRAREAVIP"
     gene            6526..7890
                     /locus_tag="PLM83_03280"
     CDS             6526..7890
                     /locus_tag="PLM83_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOK70463.1"
                     /translation="MNPWPKMPETDETHDEAVIELYQRSRDIAELAVTVESSPHADFD
                     RKLDDALTSRALGIPIMIALLGAVFWITVVGANYPSQVLAKALFWGEAKLSAAFITIN
                     APQWLHGVVVLGAYRTLAWVVAVMLPPMAIFFPCFTLLEDLGYLARITFNMDWLFKKC
                     GAHGKQALTMAMGFGCNAAGVTACRIIDSPRERLIAILTNNFAPCNGRFPTLIVLASF
                     ITAAITGPRLRSGMASLLVVAAVALGICTTLIVSWALSKTLLVGMPSSFILELPPYRK
                     PQILPVLIRSIYDRTLHVLWRAMVVAAPAGAIVWILGNLVIEGRPAIIHMSTFLQPLG
                     HAMGLDGFILTAFILGLPANEIVLPLLIMCYTSSSMLVEVEGMIHLSETLAANGWTWL
                     TAVCTMVFSVLHYPCSTTLITIWRETESRKWTVLSALIPLATAVVTCCIIASAARFCG
                     LAPL"
     gene            complement(7937..8362)
                     /locus_tag="PLM83_03285"
     CDS             complement(7937..8362)
                     /locus_tag="PLM83_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOK70464.1"
                     /translation="MLTPSLEDYLEELCRHLDCHGTVRPTDIALKLGVSMPSVTRALR
                     RLADMGMIAYRSRSDLLLTEEGRIAGDYLVRRNHALCKFLRAIGCDVCIEDQAEAMEH
                     YLMPETIDRIELLTQFLQDNENKTRFRDFCNGASNIVST"
     gene            complement(8646..8732)
                     /locus_tag="PLM83_03290"
     tRNA            complement(8646..8732)
                     /locus_tag="PLM83_03290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8696..8698),aa:Leu,seq:caa)
BASE COUNT         2088 a         2578 c         2245 g         1876 t
ORIGIN      
        1 cacctagcgt cgtcatcacg gcgcctgtcg gcggagccga actgcacggg acatgtgagg
       61 tcgaatggac ggcaacagac cctgatgatg atggagacaa cctcctgatc gaccttgcct
      121 ggacccgcga cggcgtcgag tggcacgacc tcggaaccgg actttccaac acaggcgctt
      181 tcgaatggaa caccattgaa gtaggcaaag ctggcgacag cttccggttg cgagcaagag
      241 tcactgaccc cacaggcgcg taccacgagt tcgtcacggg tgagtttacc atcatcaaca
      301 atgccccaac cgccgacttc ggcttcactc cgtcgcctgc tacaaggcgc gacgttgtga
      361 agtttaccga caaatcgaca gacgacggtc ggatagtggc atggcactgg gaattcggag
      421 acggcacgga aagctctgaa cagaacccgg agcatcggta cgccgagaag ggcgagttca
      481 caatcgcact caccgtcacc gacaacggcg agctgaaagg aaccgtggag aagagtatcg
      541 tggtggggaa cgcagcgccg atcgcagcgt tcagcttctc accggagtct cccaaggcag
      601 gcgaagtcgt gacattcacc aacgaatcca cagatgatgc cgggataatc gcgtacttct
      661 gggagttcgg agacgacacc acgagcgtcg cacccgagcc cactcacaca tacgcaacat
      721 cgggcatctt cactgtgaag ctcatggtga tagatgacga ctatactgca agcagcgtcg
      781 aacatgacgt ggaggtagtc aacgcggcac cagtggctgc gttcagcttc acgcctgctt
      841 cgccaacgat tcatgacaat gtggaattca ttgacgagtc cacggacgac ggccctatag
      901 ccgcctggca ttggacgttc ggcgacggca cggaaagcga ccagcagaac ccgagtcaca
      961 agtatgcctc caaaggcaag tacacagtga cactcactgt aactgacaac ggcggtcttt
     1021 ggggcaccgc tgaaggggag ataacagtcg tgaatctccc gccagaggta acaatagtca
     1081 aaccggaggt cggcacagtc tggacgggca tgcaaaccat cgagtgggag gctgtcgacc
     1141 ctgacgacga cgcgaacacg ctcaagatca ccctcgagta caaacccgcg ggaggtcagg
     1201 attggcagac catcgccgct aatcaggcaa ataccggtac atgcgtttgg gatacctcca
     1261 gtgtcaagca gggcggacgc tacaggataa aagtcacggc caccgatcca agcgatgcta
     1321 ccggacaggc catgagcgac gagttcacca tcgttgtact aacacacgcg atcgtggcag
     1381 ctccgaaccc ggcgaaggat ggcgtgacct tctactatga tcttgccgcc gacgcaacgc
     1441 tctacgtgta cgacatcgca ggacgccttg tttacagcgc agagcttccg gccgcggcac
     1501 atgctcatga gtggaacctc aggtcaggcg acagaccgat tgccaacgga gtctatctct
     1561 acatcgcagt aagcgggcaa gagaaaaccg aagttggaag actggtaatc agccgctaaa
     1621 cccggcttga acagtatggg cgcgggcggt cgcccacgcg gccgcccggt ggcccagcaa
     1681 ctcccgaaca ctacactcac ctaaggagga gcggctaatg cgagtgagaa tatcagcgct
     1741 ggcgtttaca ctcattctcg ccctgaccat gagcgcgtcg gcgcttgccg ccgatgaccc
     1801 ggtaggaacg gcggcgatga tggacatagg gatgggggca cgggcgcttg gaatgggcgg
     1861 cgcgcacata gctgtggcag acgattccgc cgcggtttac tataaccccg caggtttggc
     1921 cttcatgagc gaacgtcatg tcggttctct atacacacag cagtatggag ctgcggggtt
     1981 catggctgcg gcatatgccc aaaagcatgt gggcgtggga ggattgaggc tcgacgcgtc
     2041 gggaatcgag ggaacagacg agttcgccaa cgagactggc gtgttcggcg tgagtgactt
     2101 tacagtcatc gtctgtggtg gaatagaagc tctccccaat ctagggatag gagcggctct
     2161 caagtactac tcccaagcct tgcccgacaa tccgggaaag ggagtcaccg gcgacatcgg
     2221 tctcctgttt atagctccaa gcaagaaact gagagtcgga gccgtcgtga aaaacgtagg
     2281 tggcgagctg aaatactcgt cgggttacgc cgatgcgttc gaccgatccg taggtgcagg
     2341 agtcgccttc tatcccgttg acagacttct tttggcagct gatctgatat ataaggacga
     2401 gcttgaaacc agaataggcg gtgaattcca gcttggccga atcctgctaa gggcaggggg
     2461 agctttcgga agcaagcaaa actccctcac agccggcgca ggcttctcag ccagcatgtt
     2521 caccatcgac tacgcatatc agacccacag tatcctgccg gatagccaca ggctatctct
     2581 tggaatgaaa ttctgataac cagcaaacat ctcatacacc cattgcacca aagcgtagga
     2641 aatgcggggt tacgcactca agcagaaccc cgcatttccc tttctgaaat tcagcgtcat
     2701 tggccgggtc atcccactaa ctgactacat ccatgcgcag cgccttgaat acctcgaacc
     2761 atacaatact caatgtccct gtcgcaaagc agatagcaat atccacagga tgcaatacgg
     2821 taaaatggaa caaggtcctc aacgcgggaa cgcttaaaac tgtcgcaagg aacacgatag
     2881 ctccaccaac aacccacttc aaggccgggt taggccttct ccaccctgcc atcgttgtgg
     2941 attcccacga acggttggca agaatcagtc caagattcgc cagaacaagc gttgtgaagg
     3001 tcagactccg tgcctccatc tctccgcgat ccaagaggaa cagggagatg atgtagaccg
     3061 cagtcacgaa cgccatgacg ctaaggcctt gggcaattcc gatcctcatg agctcggcgc
     3121 cgaacaaggg tttggcagga tcccggggcg gcctcctcat cacgtcggcc tcttcctgct
     3181 ccatctcgaa tactacggag catgccggat cgatgatgag ctccaagaat gccacgtgaa
     3241 ccggaagaag cacgagcggc caaccaaaca acgctgggag cagtgaaagc ccggctatgg
     3301 gaatatgtat cgcgaaaatg taagccgtgg cctttctgag gttgtcgtag attctgcggc
     3361 cctgcctcac agccctaaca atggaggaaa aatcgtcgtc tagcaggact agcgaggcgg
     3421 cctcgcgcgc aacatcagtt ccccgtcctc ccattgcaat tcctatgttg gcagccttga
     3481 gcgctggcgc gtcgttcacg ccatctcccg tcattgccac gacctcaccg ttaaccttga
     3541 gcgcttccac taacctcagt ttctgctcgg ggaccatgcg tgcaaagatg caacacgtct
     3601 tcaccatctc cctcagctcc tcatcggcaa tctcccgcag ctcagatcct gtgatcacta
     3661 catccgacgg tctgaggcca atctcccgcg caatgtgctg cgcagtgcca ggataatcac
     3721 cggttatcat tacaactctg atacccgcct ggtaacactc gccaattgca tcgggaacag
     3781 ccggccttat tgggtctgca agccccacta atccgaggag ttcgaactcg aaatcatgct
     3841 gccgctcagg cagatcgccc accccatact cagccttggc tacgccaagc actcgaagac
     3901 cgtcagcggc catacggctg acctcgtcca ttaagccaca ggttttcgcc tcatcgaggt
     3961 gacacaggtc tgcaatggct tcaggagccc ccttggcagc cactacatag aaggctccgt
     4021 cggcatgtga agacatccaa acatgcgaca atgccaagaa ctcgcgggag agcgcatatt
     4081 cctccaccaa tgcccaatca tcgtgcagat gctctgtatt gatgagatgc tcatggccga
     4141 actccgttag ggccttctcc atggggtcaa acgggccttt ctcgctcgct aggatggcaa
     4201 attccacgat ctcgtgaaag aacctccacc cgcgccccta cggcctcgta gagttctcct
     4261 gcaaccgaca gtctgcttac agacatccgg ttgttccggt cttgtccaca cagagcacag
     4321 tggcggcgcc cagtgtttct atagcgggca tacgccgtgt cagcacgttt ttttgcgaca
     4381 ttctccatgc cccgagggca aggaatatcg tcaagaccac tggaaattct tcgggcagca
     4441 tagccattgc aagcgttatt cctgccagaa tcccatcaat ccagtttcct cgcgtggcgc
     4501 cgtaagccaa gactacgatt gcgaagagca cgaagcccag tatcgccagt ttgcgcacca
     4561 ttcgccctgt ttcgcggcta agggcagtct cctctagctc gactgtctgt aatgccttcc
     4621 ctatttgccc cagctcagtc tgaatgccaa tggctttaac caaggcaact ccttggcctt
     4681 gcacaaccag agttcccgag tacagatgcg gggtttcttc tcctcccgga cgtcccatgt
     4741 cgatcgactc aggccgccct tcagctgtaa ccttcctcac aggaagagac tctcccgtca
     4801 gcaacgattc atttacggac agattaattc cccatataag aacagcgtct gcaggaatgc
     4861 gatctccttc agagactagg aatatgtccc cgcacactac ctctcgtcct ggtatcctca
     4921 attgtcgccc gtcccttatg accagagcgc gtggactaga gagatctctc agggcctcca
     4981 aagcgttttc ggtcttccgt tcctggtaga tcgttatccc cattacgacg aatacgaagc
     5041 ccatgagcat agccgcctct ttagtgtcgc cgagcaagag ataaagaacg ccgcacccga
     5101 ccaagagcat gaacatcggt tctcccacta cttccaggac tattgcccat attgaccact
     5161 tcttgctagt cggaagctcg ttcggtccct cctctgccag cctcctcgcg gcttcatcgc
     5221 ccgacaagcc agtcaggttt ttcacatcgg cctcgcttgg cacattacta cctccatcat
     5281 ctttattcgg tcggtgttct ggcaggataa aaacccgagc atcgcagtcc cgcaggtctc
     5341 ttttctgctg ccagcatacg acgttgcatc tacagttacg tttgaagcac gtagagcagc
     5401 taaaaccgca cgccgacgat gtcacataga aaggatctga gcctccgccc ttctctggtt
     5461 ttgccctgta tcgacccaca aacgcgcaat gcggcaacgc ttccttcgtc tgaccgtgac
     5521 atatgcacaa tcgacaaact accccggccc gatggtgcac aagcgcgcat ctctgctcat
     5581 attctgtcaa cgcaacacga acttagcccc ggctaagtga cgcctgatgc ggaggcaacg
     5641 gcaaaatgga cactgatcat caaaaagaca acttgtgccc acttcgagat atgcccatta
     5701 gcgcagaagc ttccattgtc gaactgagag caaccggcct tgccagacgc aggttactcg
     5761 atttgggact ggttccaggc acgaagataa cgccgataag aagaagtcca ttccgcgacc
     5821 ccacagccta cctcgtcaga ggaaccatca tagcccttcg gcaagaagac tctgcccaga
     5881 ttatcgtcag gaagagtgac tgctgatgaa aaaagccatg ccaactccgc atactctatc
     5941 gaaagggctg cgatccttga gccggcccga aggtgatatt cccatagttg gccttgcagg
     6001 caaccccaat acgggcaaga gcactgtgtt caacgccctc acaggccttc gccagcatac
     6061 aggcaactgg ccagggaaaa cggttgggca ggcgagcgga ttgcttagat acagcggcga
     6121 tacaatccgc ctggttgacc tgccagggac gtattctctg ttcgccacgt cggaagagga
     6181 gagaatcgcc cgggatttca tatgttccaa tacggcctca gcaatagtga tagtagtgga
     6241 tgccacctgt ctcgagcgca acctcaacct cgtcctccaa gtgctcgaga ttacagccaa
     6301 agcaatcttg tgtgtcaatc tgatagatga agctaggcaa aaaggaatcc gcgtgaaccg
     6361 agaagtcctc agtcgcgaac taggaattcc agtggtcctg accgcggccc gctccggaga
     6421 gggattagat gagctgaagc gcacgatact gcattcagtt ggccgaggag agcccgtggc
     6481 tgcaaatcac caaacctccg ttagggcccg agaggcggtg attccgtgaa tccgtggcca
     6541 aagatgcccg aaaccgatga gacgcatgat gaggcagtga tcgagctgta ccaacgttcg
     6601 cgcgacatag cagagctagc cgtaaccgtg gaatcatccc cccatgcaga ctttgacaga
     6661 aagctcgatg acgcactcac atcgagagct ctgggcattc ctataatgat cgctcttttg
     6721 ggagcggttt tttggattac agtcgtaggc gccaactatc cctctcaggt gctggccaag
     6781 gctttgtttt ggggagaagc gaagctcagt gcggccttca taaccatcaa tgctcctcaa
     6841 tggctgcacg gggttgttgt gctcggcgcc tatcgcaccc ttgcatgggt ggttgccgta
     6901 atgcttccgc ccatggcgat cttcttccct tgctttactt tactggaaga cctgggctat
     6961 cttgcgcgga taactttcaa catggactgg ctcttcaaaa aatgcggcgc ccatggcaaa
     7021 caggccctca ccatggccat ggggtttggg tgcaatgctg caggagtaac agcttgcaga
     7081 atcatagact cgccacgaga gcgattgatt gccatcctca cgaataattt cgcgccttgc
     7141 aatggccgtt tcccaaccct catcgtcctg gcctcgttca tcaccgccgc catcactggc
     7201 ccgcgattgc gctcgggcat ggcttcgctt ctggtagttg ccgcagttgc gcttggaata
     7261 tgtacgacat tgatcgtttc atgggcactg tcgaaaacgc tccttgtggg gatgccgtct
     7321 tcattcatct tggaattgcc gccctacaga aaaccgcaga tactgccagt tctcatccgg
     7381 tcgatctatg atcgaacgct acacgtgctc tggcgcgcga tggtagtagc tgcgcccgca
     7441 ggcgcaatcg tatggatcct tggcaatctc gtaatagaag gccggccagc aattattcac
     7501 atgtccacgt ttttgcagcc tctcggacac gccatggggc tagacggttt catactcaca
     7561 gctttcattc tgggtttgcc cgctaacgaa atagtgctcc ccctcctgat catgtgttac
     7621 acgtcttcat cgatgctagt ggaagtggaa ggcatgattc acctgtcaga aacgctcgct
     7681 gcaaatggat ggacatggct gactgccgta tgcaccatgg ttttctctgt tcttcactat
     7741 ccatgcagca caaccttgat aacaatctgg cgggaaaccg aaagccgaaa gtggacagtt
     7801 ctttcagccc tgattccgct ggctacagcc gtcgtaacct gttgcattat cgcttctgca
     7861 gcgcggtttt gcggccttgc acctctctga cagcactatg ttcagcgaaa gcttagccaa
     7921 acaaggaaac cgggggctat gtggacacga tattcgatgc tccattgcag aagtccctga
     7981 acctcgtctt gttttcgttg tcttggagaa actgggtaag aagctcaatc ctatctattg
     8041 tttcaggcat aagatagtgt tccatggctt cagcttgatc ctctatacaa acatcacatc
     8101 ctatcgccct gagaaacttg cacagcgcgt gattccggcg cacaaggtag tcacctgcta
     8161 tcctcccttc ctctgtcaac aacaagtcgc tccgggatct atatgcgatc atgcccatgt
     8221 cggcaagcct tcggagtgcc ctggttacag atggcataga tacacctagc tttagtgcga
     8281 tgtctgtcgg gcgcactgtt ccatggcaat caagatgacg gcacaattcc tcaagataat
     8341 cctctaggct aggcgtgagc atttcatcca cctacctctc ccaatcagac tctatttctg
     8401 acagtcactc tatgcgcatc ctccgattca gttcttgaaa atcgatgccc atagcttact
     8461 ctgctgtcgc ctaacagatg gtccgtcttt gtacatcaga atccccggcg ctcgtgtctg
     8521 cattacgcgc aaaccggcta caacgttccc tcctgaaagg aataacgaag agacggacac
     8581 cacatggcta ttcgccaata gtgtccgtca cagtctgcac atccacatat gtaattcgaa
     8641 aaagatggtg ccgaaggggg gactcgaacc cccatgacct cgcgatcgga tgattttgag
     8701 tcatctgcgt ctgccgattc cgccacttcg gcgcgtcact attctaccac gggcgcccgc
     8761 ttggtgcaat aggacgcagc agtcgag
//
LOCUS       DAOSAI010000056         8677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5312_length_8677_cov_3.658200, whole genome shotgun sequence.
ACCESSION   DAOSAI010000056 DAOSAI010000000
VERSION     DAOSAI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5312_length_8677_cov_3.658200"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PLM83_03295"
     CDS             <1..921
                     /locus_tag="PLM83_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOK70465.1"
                     /translation="KAILVAEKLAMDRSVIGVIGPMNSATAQAALPTYQRAGLAVISQ
                     SATNPDLTEMGYTVMHRICPRDDAQGPASARFIIEVLKAKTVYIIDDKTTYGQGLSDQ
                     VEPALKAAGVRVLRGQISMEDRDFSPILTRVKSSAPDLVYLGLANPAQAASLIKQAAG
                     LGLKPVFMGGDGLKERDQLIDGAAGLAEGMYVTSIGRDIREVPEARNFIRKFESKYGA
                     LSVFGGQSYEAANILIDAARRAHSDPMKITRANVLAALGKTRGYKGILGFPISFDSKG
                     DVVGASIFVYQVKGKEFVPVKEYPATVRAK"
     gene            1010..1936
                     /locus_tag="PLM83_03300"
     CDS             1010..1936
                     /locus_tag="PLM83_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOK70466.1"
                     /translation="MEQFLAQVPQQIVNGITLGSVYALLALGYSMVYGVLKMLNFAHG
                     DVFMVGAFAGWAVLSLFEQNGLIAMPALALAAMALGAIVATALLGAAIERFAYRPLRG
                     GSRLAPLISALGVSIILENAAMLLTSGRAKTFPTELLIPQDMVVHVAGAAISATRLAM
                     LGTSILAMVILDAVVLRTKFGRAMRATSQDAEAAEYMGIRTSLVISATFMLGSALAGL
                     AGVMIGLYYTQVDFMIGFSAGMKAFTAAVLGGIGSFRGAVVGGLLLGLAESFGVLIAP
                     PVYKDAIVFAVLILTLILRPHGLYGKPQQARA"
     gene            2006..4885
                     /locus_tag="PLM83_03305"
     CDS             2006..4885
                     /locus_tag="PLM83_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HOK70467.1"
                     /translation="MSNECVNTTEKHPHSASLRQTEAEQAQVAPEAATRASNSAKAAD
                     GPNLAGGRLRIGLLERLGLWQSGRVPIERALVFLGYAVLLPVPLITKNYYLMRAGGSV
                     GIYLMLAAGLSIVAGQAGLLDLGYAGFYGIGAYVYALLASPQFGIHMPFFVAAAASVA
                     AAVIAAFAVSLPTLRLHGDYLAMVTLGFGQIVRILLNNMDRPINITGGPNGIVAIDPP
                     HIFGSSLASMESQYILIWIFAAAVLIGISRLAGSKVGRAWNALRENETAAECMGVNVR
                     KYRVSAFAGAAAIAGLAGALFASWQGAVFPQNFTMSETIALYCMLVLGGTRSVPGIML
                     GVLLLQVLPEVLRAYSVYRMLIYGFALVLLAIFRPQGLIATGERLVGVDPTQAHGAHP
                     GPGICLGTNANAGLAERTTDDAVSASSCPIPDNGGEPEAIRSASTSASPGQATVLEVT
                     GLSCRFGGVQALSDVSFTVKRGEILGIIGPNGAGKTTLFNLITGLANPSAGDIRLYGQ
                     SIVGKSPHEVAAMGAARTFQNIRLYETQTVLENVLAGRHLKQDAHLWEVLFRTKSHVR
                     KEEEDILCVRHILSDMDCGLEKRESDIVAELSYPDKRRVEMARALATGAKFILLDEPA
                     AGMTSEEIAAMAREIVALRDRGYTIVVIEHHMDLIASACDRVIVLDHGEKIAEGSCSE
                     VAKDPEVIRAYFGSSAAPDRKPAETLASNAMSTSSAATSGLPTPLLELIDVNSSYGPA
                     VVLQGANLYVNPGEAVALLGVNAAGKSTLLKTIAGRLRPTRGEIRFMGERIDGRETNE
                     IIRMGMGVVPEGRRVFQELTVMENLEVAADALGDPEGAREGIDQALSLFPVLADRRSQ
                     LAGTLSGGEQQMLAIARALVTSPRLICMDEPSMGLAPIMVERVMGAIAEINRAGTAVL
                     LVEQNAKAALSVADRAYILKDGRTSAPSNPNQILSGEAGKVASIF"
     gene            complement(4984..5769)
                     /locus_tag="PLM83_03310"
     CDS             complement(4984..5769)
                     /locus_tag="PLM83_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOK70468.1"
                     /translation="MEAYEQNALSSTEHVIGVMSGKGGVGKSSVTALTAVALARMGYK
                     VGVMDADITGPSIPKMFGVHGKLDSAETGLFPAQTERLGIRIVSLNLLLDREDRPVVW
                     RGPLLAGAVKQFWTDVSWGDLDFMIIDLPPGTGDIPLSVMQSIPVEGLITVSSPQDVA
                     ALVVRKAVNMAEMMDKPIIGLIENMSYIKCPGCGETMYPFGKPAGAGFASEMGIPFIG
                     EMPIDPALSEAADRGEIEMYESPIMQGIAEAVVRHVQASAQEE"
     gene            complement(5876..6460)
                     /locus_tag="PLM83_03315"
     CDS             complement(5876..6460)
                     /locus_tag="PLM83_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOK70469.1"
                     /translation="MGRPPKVRCVEMIPPVRTFCPCGNWPECRAHRGADERIDMTVDE
                     MEAIRLKDLEGCEQTECADRMNISRGTFQRILSSARWKVAKALTEGLILQISGGEYCL
                     AQSTIICLDCGHAWPVCRPNADSKDECEETVTAEDGTVSGSCPECRGTNLVEKGGECS
                     GPGGREDCPRRRERGRRGCSHRSGAEESAAKSTD"
     gene            complement(7066..7434)
                     /locus_tag="PLM83_03320"
     CDS             complement(7066..7434)
                     /locus_tag="PLM83_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOK70470.1"
                     /translation="MDKPVTTAHARSELMQLLLVFRDEREWGQFHTPKNLAIALVAEA
                     SELLSLFQWTKDAELDERCREKRTEIEQEIADIYIYLLFLANELGIDLDEVAAEKMKL
                     NAEHYPANKAKGNARKYTEL"
BASE COUNT         1835 a         2511 c         2386 g         1945 t
ORIGIN      
        1 aaggcgattc tggtagcgga aaaactcgcg atggaccgtt cagtcatcgg tgttatcggg
       61 cccatgaaca gcgccaccgc ccaggcagcc ctcccaacgt atcagagagc aggccttgcc
      121 gtcataagcc agtcggcaac gaatcccgac ttgactgaga tgggctacac ggtgatgcac
      181 aggatctgtc cgcgcgacga tgctcagggg ccggcttcag cccgcttcat aatagaagtc
      241 ctaaaggcaa agacggtata catcatagat gacaagacca cctacggcca aggcctttca
      301 gaccaggtgg aaccggccct caaggctgca ggcgttaggg ttctccgagg gcagatatcc
      361 atggaggaca gggacttctc ccccatcctc acccgggtca aatcatccgc gcccgatttg
      421 gtctacctcg gcttggccaa ccccgcacag gcggctagct tgatcaaaca ggccgcgggc
      481 ctcggactca agccggtatt tatgggcggc gacgggctaa aagagaggga ccagctgatc
      541 gacggcgcag cagggcttgc cgaaggaatg tatgtcacct ccataggccg agacatcagg
      601 gaggttcctg aagcccgcaa cttcatccgg aagttcgagt cgaaatacgg ggccctgagc
      661 gtcttcggcg ggcaatcgta cgaggccgcc aatattctga tagatgcggc caggcgagcc
      721 cattccgacc cgatgaagat aacccgggcc aatgtcctcg cagcgctggg caagacccgc
      781 ggctacaagg gcatcctcgg attcccgata agtttcgact caaaaggcga tgtggtggga
      841 gccagcatct tcgtctatca ggtgaagggc aaagagttcg tgcctgtgaa ggaataccct
      901 gcgacggtgc gagccaagta ggccgacgct acgctcgtcc tcaaaggtta gcccaagggc
      961 ccaattccgt aaaacgcggc gtccgcaaag ctcaattgag gtgaatttca tggaacagtt
     1021 tctggctcaa gttccgcaac agatagtaaa cgggatcacg ctcgggagtg tatacgccct
     1081 cctcgccctg ggctactcca tggtttacgg ggttttgaag atgctcaact tcgcccacgg
     1141 tgacgtcttc atggtcgggg ccttcgcggg ctgggcagtc ctctccctct tcgaacagaa
     1201 tgggctcata gccatgcccg ctctggctct ggctgcgatg gcgctggggg ctatagtcgc
     1261 gaccgctctt ctcggcgcgg caattgaacg gttcgcttat aggccgctcc ggggcggttc
     1321 tcgcctcgcc cccctcatct cagcccttgg cgtttccatc attcttgaga acgcagcaat
     1381 gctcctcacg tctgggaggg ccaaaacgtt cccgactgag ctcttgatcc cgcaggacat
     1441 ggtcgtccac gtcgcaggcg cggccatctc tgcgacacgt ctcgccatgt tgggcacgtc
     1501 catcctggcc atggtcatcc tcgatgccgt ggtgctgagg actaagttcg gccgggcgat
     1561 gcgagcgacg agccaagacg cggaggcggc ggaatacatg ggaatccgaa cgtcgctggt
     1621 tatatccgcc acattcatgc tcggctcggc cctagcgggg ctggcggggg tgatgatagg
     1681 cctgtattac acccaagtag atttcatgat cgggttttcg gcaggaatga aggccttcac
     1741 cgcggcagta ttggggggaa tagggagctt ccgtggagct gtagtaggag gactcctgct
     1801 cgggctggcg gaaagcttcg gggtgctgat cgcgcccccg gtctacaaag acgccatcgt
     1861 ctttgctgtg ctaatactca ccctcatcct ccgcccccac ggactctacg gtaaacccca
     1921 gcaagccagg gcgtgaggag caagcacgat ggccaaccga tgaggcaacg gtatacaatc
     1981 actaatctga aaggcggcat agccgatgtc caatgaatgc gtgaatacca ctgaaaagca
     2041 cccccacagc gcatcgctcc ggcaaacaga agccgagcag gcgcaggtgg caccggaagc
     2101 ggccactcgg gcgtcaaatt ccgccaaggc agccgatggg cccaatctcg ccggcggacg
     2161 gcttcgaatc ggacttttgg agcggttggg cctgtggcaa agcggacgcg tcccgatcga
     2221 gcgcgccctc gtcttcttgg gctatgccgt cctcttgccc gtccccttga taacgaagaa
     2281 ctactacctg atgcgtgccg gagggtctgt cggcatatac ctgatgctgg cagcggggct
     2341 ttccatcgtg gcgggacagg caggcctcct cgatctgggc tatgcgggat tctacgggat
     2401 cggagcatat gtatacgcgc tgctcgcatc cccccagttc gggatacaca tgcccttttt
     2461 cgtggcagcc gcggcatctg tggcggcggc ggtgatcgcg gcatttgccg tgagcctgcc
     2521 gacccttcgt cttcacggtg actacctggc catggttacc ttggggttcg gccagatagt
     2581 ccgtatactc ctgaacaaca tggatcgccc tatcaacatc acaggcggcc caaacgggat
     2641 agtcgcaata gatccgccgc acatcttcgg ctcgtcttta gcgtcaatgg aatcgcaata
     2701 cattctcatc tggattttcg ccgcggcggt gctgataggc atatcgaggc tcgcaggttc
     2761 caaggtgggc cgggcatgga atgcgctgcg tgagaacgag actgccgcgg aatgcatggg
     2821 cgtgaacgtg aggaaatacc gagtgtcggc cttcgcaggc gctgcagcaa tcgccggact
     2881 tgccggagca ctctttgctt cctggcaggg ggcggtcttt cctcagaatt tcactatgag
     2941 cgagaccatc gccctctact gcatgctcgt gctgggcggg actcgaagcg tgccagggat
     3001 aatgctggga gtgctgcttc ttcaggtgct gcccgaggtt cttcgcgcat acagcgtcta
     3061 tcggatgctc atatacggct tcgccctggt actgctcgcg atcttccgcc cccaaggctt
     3121 gatcgccaca ggcgaaagac tggtcggggt cgacccgacc caagcacatg gcgcacaccc
     3181 tggcccaggt atatgtctgg gcacaaatgc gaacgcaggt cttgccgaaa gaacaacaga
     3241 tgatgccgtg tctgcttcat cctgccccat acccgataat ggcggcgagc cggaagctat
     3301 ccgcagcgcg agcacatcgg cgtcgccagg gcaagccaca gttctcgagg ttacggggtt
     3361 aagctgccgt ttcgggggag tgcaggctct ttcggatgtg agtttcacgg tcaagcgcgg
     3421 cgaaatcctc gggataatcg gtccaaacgg agcgggtaaa acgacccttt tcaacctgat
     3481 tacaggcctt gcgaacccaa gtgcgggcga catcagactg tacgggcaat cgatcgtagg
     3541 caaatccccg cacgaagtgg cagcgatggg agcggcgcgg acgtttcaga atataaggct
     3601 ttatgaaaca caaaccgtcc tcgagaacgt cctcgccgga cgccacctga agcaagacgc
     3661 acacctttgg gaggtgcttt tcaggacaaa gtcccatgtt cgcaaggaag aggaggacat
     3721 cttgtgcgta aggcacatcc tttcggacat ggattgcggc ctcgagaagc gggaatctga
     3781 catcgttgca gagctttcat accctgacaa acgccgagtg gagatggccc gagcgctggc
     3841 cacaggtgcg aagttcatcc tcctcgacga acctgccgcc gggatgacgt cggaggagat
     3901 cgcggcgatg gcgagagaga tcgtggcgct tcgggaccgc ggctacacca tcgtcgttat
     3961 cgagcatcac atggacctga tcgcgtcggc gtgcgatagg gtgatcgttc tggaccacgg
     4021 cgaaaagata gctgaaggtt catgtagcga agtggcaaag gatccggagg tcatccgcgc
     4081 ctactttggc agttccgcgg cgccagatcg aaagccggct gaaaccttgg catcgaatgc
     4141 catgtcgacg tcgtcagctg ctacgtcggg gcttcccacg cccctcctcg agctgataga
     4201 tgtcaattca tcatacggcc ccgcagtggt cctgcagggt gcaaacctct acgtcaatcc
     4261 cggcgaagca gtggcgcttt taggagtgaa cgctgcaggc aagagcaccc tgctaaagac
     4321 tattgcagga aggctccgcc ccactcgggg ggagatccgg ttcatgggcg agcggataga
     4381 cggacgggaa accaacgaga tcatcaggat gggcatggga gttgtgccgg aaggacgaag
     4441 ggtctttcag gaactcacgg tgatggagaa cctggaagtg gcggctgatg ccttaggcga
     4501 cccggaaggg gcccgggagg gcatcgacca agcgctctcc ctcttccctg tcctggcaga
     4561 caggagatca cagctcgccg gaaccctgtc cgggggcgaa caacagatgc tggccattgc
     4621 aagggctctc gtcacatcgc cccgcctcat atgcatggat gaaccctcca tggggctagc
     4681 acctataatg gtggaaaggg ttatgggggc cattgccgag ataaaccggg ctggaaccgc
     4741 ggttcttctg gtggagcaga acgcgaaggc cgctctttca gttgcggacc gagcctacat
     4801 tctcaaggac ggcaggacat cggcgccttc caatccgaac cagattctgt cgggcgaagc
     4861 tggaaaggtt gcaagcatat tttgattgat ccaagaagca gggctatctt tgcagaccgc
     4921 cctgcttcac tctctctttt caggagaaac ttgtgtgaat ctctctcgcg cagcgccgac
     4981 gcgctactct tcttgcgcac tcgcttgtac gtgccgtaca acagcttcgg cgatcccctg
     5041 catgattgga ctctcataca tctcgatttc gcccctatcg gctgcttcag atagagcggg
     5101 atcgataggc atttccccga tgaacggaat tcccatttca gaggcaaatc cagcgcctgc
     5161 cggcttccca aagggataca tcgtttcgcc gcatccaggg cacttgatgt agctcatgtt
     5221 ctcgataagc ccgattatgg gtttgtccat catctcggcc atgttcaccg ctttgcgaac
     5281 gacgagagcc gctacgtcct gcggggatga aaccgttatc agcccctcga ccggtatcga
     5341 ctgcatgact gaaagcggaa tgtcgcccgt tcccggcgga aggtcgatta tcatgaaatc
     5401 gagatcaccc cagctaacgt ctgtccaaaa ctgctttact gctccggcaa gaagaggacc
     5461 gcgccatact actggcctgt cttccctgtc caaaagcaag ttgagggaga ctatcctaat
     5521 ccccagcctc tctgtttgcg ccggaaagag ccctgtctcc gcagaatcaa gtttgccatg
     5581 aacaccgaac atcttgggta tgctagggcc ggtaatgtct gcgtccatta cgcctacttt
     5641 gtatcccatt cgggccaaag ccactgcagt gagcgccgtc actgacgatt tgcctactcc
     5701 gcctttgccg ctcattactc caatgacatg ctctgtgctg gatagcgcat tttgctcgta
     5761 tgcttccatt cttccgtcct tcttgcgtct ttagtttcgg tatacccacc ctcgtggggc
     5821 agcacaacca atacttcagc cactatcacc gcttccgtag atcgagcaat gtcgatcagt
     5881 cggtgctttt ggctgctgac tcttcggcgc ctgagcgatg cgagcaacca cggcgtccac
     5941 gctctcttct gcgcggacag tcttccctac cgccaggccc cgagcattct ccgcctttct
     6001 cgacaaggtt ggtccctctg cactcaggac atgaacccga tactgtgccg tcctcggcag
     6061 ttacggtctc ttcacattca tctttgctat cggcgtttgg gcgacaaaca ggccaagcat
     6121 gcccacagtc gagacagatg atggtcgatt gggccaggca gtattcaccg ccggatatct
     6181 gcaagatcaa gccttctgtg agagccttcg ccactttcca gcgggcagac gagagtatcc
     6241 tctggaatgt gcctctcgag atattcattc tgtctgcaca ctctgtctgt tcgcagcctt
     6301 ccagatcctt gaggcgaata gcttccatct catccactgt catatcgatt cgttcatctg
     6361 ccccacgatg agcacggcat tccggccagt tgccacacgg gcaaaaggtt cttacaggag
     6421 ggatcatctc cacacatctc accttgggag gtctgcccat gttctcgccc ctcttaagat
     6481 gcatttgtga aatcgcgcac attccacaag gatactacat gcattttgtg catatgtcaa
     6541 taacatcgag caagtatatg cacgctggtt ctagttttgc tggcaggacg cgacgtgatt
     6601 ccattttggt aggcggcagc gagccctaat ccgggttgga tgaggtcttg ttaattgaag
     6661 ctcgtgcctt gcacaacggg cctcaagtcc ctcttcgcgg ataatcctga ggctttcgta
     6721 cagggcgagc agtgaggtgg cagtctcctt cgcccagtgg ctgtgagcat ggcaatgtcc
     6781 agataccagc ggtcttccgc gacctgagga tcccaccggg caatcgccta tggtaatagg
     6841 cgcaagctct gacgggtaca acagacactt ctgtgttccg gtgtagcggc gtccgctcca
     6901 catcctgcat tatgtcagcc gtgcgctaac cggacagccc ctttacgcag acgaagacct
     6961 gatcttcttg ttcgatgaag cctaccaggg atctttccat gcccgtggac cagcgttaac
     7021 accccctggc gactgaaggc aaaagctgca tcgagccgtg cattgctaaa gctccgtata
     7081 tttcctggcg ttgccctttg ccttgttagc gggataatgc tcggcgttga gcttcatctt
     7141 ctctgcagcc acttcatcaa ggtcaattcc aagttcgttg gcgaggaata gaaggtatat
     7201 gtagatatcg gcgatctcct gttctatttc ggtccgcttt tcccgacatc tctcatcgag
     7261 ctccgcatct ttggtccatt gaaagagcga aagcagctcc gacgcctccg ctacaagcgc
     7321 tatcgctagg ttcttggggg tatggaactg gccccactct ctttcatcgc ggaacacgag
     7381 cagcaactgc attagttcgc ttcgggcatg tgctgttgta accggtttgt ccatcaaccg
     7441 aaaagagcct cctcaaaatg gtttcgggtt gcctggtcga ggaagtatac gtaggtgcct
     7501 ttcatgccgc gggatagagg tgtccctcca agactcatcg gctgttcttc gcgtggcatg
     7561 gatctacttg cccgctactt ccttatagat ggtctgaaag tacctcccat tgagctatct
     7621 tctggactgt gggttatcgt tgtactgctg gtgaagttgc aggcagtccc gagagcatgc
     7681 atgccccaat gcgcgttcat gctcaatctg ggcattctga taggccctac aagctgcgac
     7741 ctctgcagac atttccacgg caagcaagcg cctgccatgt cttgttcatc caacccacgt
     7801 agcggcagag cacccacaac tgccgcacat gatcctgcac acgtttttgg ggcaagcctc
     7861 cttacgccag ctgctcatct tgtgcgccga gtcataacaa tacctgccag cgtcaaggca
     7921 attcccgtta ttgcaatcgg aatatagata tgatctgcga tgcttaccac aaacctatca
     7981 attgtagaaa gtacaacata tatcagcacc cctgctgatg tagtgagact cgggttcttc
     8041 ttcactgctg cttcaccttc accaactgca ttatttacgc acaaaccaag gaatctgccg
     8101 cttcagtata gcagaacagt ctagggcgtc aaacaggctc cacctggagc ttcaatcagt
     8161 ctcgatgtct ctatcggccc catgtttctt ggattgagca acgtccttgc ggcgcccatc
     8221 tctgaacgtc tgtgcttata cgatattctc gataataaac agcaataatg ccatcgggca
     8281 ttgggctcca atgcctagag ccactatctc ttgaaggatt caatcattat ttatctaatt
     8341 ttgtaatgta cgcttgcgtc ttgcaatcgt ccctaagtct gctcttgatg gatggtgttg
     8401 gtatgtcgac ttctagttca gatatggcaa atgccactca acgctatatg gcaaggattc
     8461 acgggatgcc tctcctcttc cccgagagcc gtctcactgc gcaactgcgt ctctcgggac
     8521 tgtctgatga ggagattatg gagcatgtct atgtcaataa ttcgttccaa tttcccacta
     8581 ggcgcaagat acgcgggtca ctcgggtgat tttatcgcgt ctcgacgcgc tcagccctga
     8641 gctcttgaaa gtgcttgccc acggcccttc cgacttg
//
LOCUS       DAOSAI010000057         8501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5436_length_8501_cov_3.288184, whole genome shotgun sequence.
ACCESSION   DAOSAI010000057 DAOSAI010000000
VERSION     DAOSAI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5436_length_8501_cov_3.288184"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="PLM83_03325"
     CDS             complement(<1..1425)
                     /locus_tag="PLM83_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOK70471.1"
                     /translation="MRNRGLGRRAAAALVLILCLAFAGQAAFGASERRVDLDLVDAEL
                     ADVFRALAELGDMNIVLDPAVRGTLTIRLHGLAVEDALRLVAYTTGVKYRVEGSTMIV
                     LPPGAVSTSLEPLGIEKFSLTYARTTDVIGPARLVAGGAKLEADERTNSIIVCGSAEE
                     LRAIGDVLRMLDVPVQLPPEAIASLADAGLSSGHESLEIIRLQHAPAQGVADLLALVV
                     PADRMRVDSRTNTIVVIIDEASRERAMKIVSEVDVAEPADDGGFSSVPRKGVNLQTEA
                     VEAETVKVIKLAWAPVDKVREALSVIIDLSKVGMDSRTNSLIVNATPEQIARIEEIVT
                     LLDVAVVAAEKQPAPAHDGIHMFKLEHASAKSVRDVLTMILPPEKMQVDERTNTVVIM
                     ADDDTCARVVELVETLDVETAVDQALIGEEKIVETIPLAHASLDAIRDALALVVPKEA
                     VVIDERTRSVIVKGTRSQRERAREI"
     gene            complement(1422..2036)
                     /locus_tag="PLM83_03330"
     CDS             complement(1422..2036)
                     /locus_tag="PLM83_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70472.1"
                     /translation="MALDLSRESRNEWPSPDGETEAKKRKYRWTALIVALLLVASFSG
                     YMWFLKNRKPDRYPGGSDVVTGGRVAARLSALLDQSHEYGWSRVEEEFSPVGHGRWDP
                     FKTLLPDPVQIVPVEPRTPIPALPEPEDSAPCEPALPKVRLTGVLKSGDRAIALLSVD
                     GRSVMTRVGDAPFDGAEVRSIDDKSITISFGGTDFTYQLGGERR"
     gene            complement(2020..2640)
                     /gene="pilO"
                     /locus_tag="PLM83_03335"
     CDS             complement(2020..2640)
                     /gene="pilO"
                     /locus_tag="PLM83_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOK70473.1"
                     /translation="MSLSTEVNPKQAKTFIVLIILAAAGLLYMYVFAPQARKLADART
                     RLDEANSVLTVDLAKIRGAGRSEEELELAMAQLENFERLIPREDRIHYVLRDMESVAL
                     SNHVAIDSLTIASGMPRGQYVEIPMTLNVSGTYNNIIGFLDGIADIERVMNVHSLSFG
                     GGRELWREGGETQDRAVRSPDAYISSTIQVTTYARPRGDDQVGAGS"
     gene            complement(2646..3191)
                     /locus_tag="PLM83_03340"
     CDS             complement(2646..3191)
                     /locus_tag="PLM83_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOK70474.1"
                     /translation="MSKINLLPQREPEESGISPAKLGLIIVLEIAIVGASALYAYRHF
                     FIQQLEEDIDRIEAQIGAIRADVEAVEKVQRRTADIRKRIDEAEAISAEGLASGRVMR
                     EVRDIIPRDIWLSSLSINPDGGVSLAGDTFSIESVARFALAIEDSPMFGEAHVGSVSL
                     AAEGDYEVYDFNIDCMLDARR"
     gene            complement(3191..4318)
                     /gene="pilM"
                     /locus_tag="PLM83_03345"
     CDS             complement(3191..4318)
                     /gene="pilM"
                     /locus_tag="PLM83_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOK70475.1"
                     /translation="MPLRLFSRKSRSMVGVDIGTSRVKVVELVGRGDSVELTRAAIEL
                     APPGAIRDGQIVSPELVAEVIQRGFAKGRIKPGEVVAAIAGRGVVVRHVKFPRMPEEE
                     LREAIKWEGQQHIPMPIEEAVVDFEIIDDYSGSDEAEMEVMLVAAPRKLVESHVQAIE
                     LAGLTPIAIDVQPFAVLRALRYEPGDKTLHPNDRSATAYVDIGAGTTDILIAQGERLR
                     FTRIVPLGGNTFTNAIAETLGVSFEEAEAIKIAKGRVCVDGCGEPCADREEQDISDAI
                     ANVAADVAMDIRRSLDYHDLQLEGRSQDAGVTRVVLTGGASVMEGLDCYLESELGLAT
                     VMGDPLKSILVNQRLANKEELSRLGPMFAVGIGLALREVVD"
     gene            complement(4383..5126)
                     /locus_tag="PLM83_03350"
     CDS             complement(4383..5126)
                     /locus_tag="PLM83_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOK70476.1"
                     /translation="MISLFSFILGLVIGSFLNVCICRLPSGGSIVNPGSSCPYCGTPL
                     APRDLIPVASYLVLGGKCRYCGEPISIQYPVVEVATGLLYAAIVWANGLTFESLSLLV
                     FVSLVIICAGTDLNTGLILNSVTFPGVVIGVALSFIVPSMTPLQSLLGLLVSGGVVWI
                     MYVVTRGRGMGGGDVKFMAMVGAFLGWDRGLLTLFLGALIGSVVGIARIVSGKQERRD
                     PIPFGPFLSIGAVISVFFGAKLLGFLHLW"
     gene            complement(5364..5711)
                     /locus_tag="PLM83_03355"
     CDS             complement(5364..5711)
                     /locus_tag="PLM83_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOK70477.1"
                     /translation="MKRDQRGFTLMELMIVIVIIGVLAAIGVPAYNNYVKKAKAATCQ
                     ANRRTLDTAVGLYYAEHGDYPTADGTEGTSTLQSVLKEYLDNADELKCPEDGQYSLDG
                     ASHKVVCSLAGHE"
     gene            complement(5918..6400)
                     /locus_tag="PLM83_03360"
     CDS             complement(5918..6400)
                     /locus_tag="PLM83_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOK70478.1"
                     /translation="MRPIATTVGMLRSIASCILAKEYEVIGMRQDARGFTLVELMTVI
                     VIIGVLSAIGIPSYKGTVDKAKTEACKANRRILSTAVATYYLEHSTYYKLDSAGNPRD
                     EVIEVADLTDYVENAEELKCPAGGNYTIIPGNDGKASSVVIRCDHEGHGDFVVFGTSD
                     "
     gene            complement(6408..7643)
                     /locus_tag="PLM83_03365"
     CDS             complement(6408..7643)
                     /locus_tag="PLM83_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOK70479.1"
                     /translation="MASLSWSYRGRDMSGQVVEGTMEAENPETVAERLRSEGYVPVMI
                     TEAKRSVFSADASAASGKKVQAATSLDTMQLSLISRQMSTMVAAGIPLVQTLAILSQQ
                     IEDPRIAELINEVRRSVESGETFAASLAKHPGCFPPLFVHMVEAGEASGTLDIVMNRV
                     ANYYEQQVKIRSQLKNALVYPIILVAVALVVVVILMAFVLPTFIDMFEGFDMELPWVT
                     RAVFGYGQSVQRAWYLHFGIPIVAIVWFVSYVKGAGKSWWDHTAIRLKISGPIIRKSQ
                     TAQFARTFAMLTQSGVPMLQSLDILERLSDNAVVRETVEASKISVRDGAGLARPLRQS
                     QIFPPMLAHMVAVGEETGNLDGMLNKTADFFDQEVEYEVQRVTTAIEPIMTLIIGLFV
                     ALIVASVMIPMFNMASFGM"
     gene            complement(7662..>8501)
                     /locus_tag="PLM83_03370"
     CDS             complement(7662..>8501)
                     /locus_tag="PLM83_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOK70480.1"
                     /translation="RFRVNAYTQRGSVGAAFRVIPTRIRSLEELGLPPVVKTFADKTR
                     GMVLVTGPTGSGKSTTLASLIDIINSERACHVITLEDPIEYLHKHRKSMINQREVGTD
                     TKSFAAALRAALREDPDVILVGEMRDLETTSIALTAAETGHLVFATLHTNDTVQTVDR
                     IIDQYPPHQQQQVRVQLAAVLQGIVSQILLPRADGQGRIVAVEVLVANPAVRNLIREG
                     KTHQIYSVLQTGAKQGMVSLDMYLVDLAKRGIITYDDALSHATNPDEMRRLGQFAQPI
                     MRL"
BASE COUNT         1946 a         2601 c         2063 g         1891 t
ORIGIN      
        1 gatctcgcgg gcacgctcac gctgcgaccg ggtgcctttc acgattaccg accgcgtcct
       61 ctcatcgatt acgacagctt ctttcggcac caccaaagcc agggcatctc gaattgcatc
      121 caaagaggca tgggccaagg ggatagtctc aacgatcttc tcttccccga tgagtgcctg
      181 gtcgaccgca gtttctacat ccaaagtctc aacaagttcc acaactcgag cgcaagtatc
      241 atcatccgcc attatcacca cggtgttcgt cctctcgtcc acctgcattt tttcaggggg
      301 caggatcatg gtaaggacgt ctcgcactga tttggctgat gcgtgctcga gcttgaacat
      361 atgtatgcca tcatgggctg gtgcaggctg cttttccgcg gctaccacag ccacatcgag
      421 aagagtcaca atctcttcaa tccgggctat ctgttcaggc gtcgcattta caatgagcga
      481 atttgtgcgt gaatccattc caaccttgga aaggtcgatg atgacactga gagcttcacg
      541 gaccttgtcc acgggggccc acgcaagctt gatcaccttc accgtctcgg cctcgactgc
      601 ctcagtctgc aggtttacac ccttcctggg aacactcgaa aaacctccat catcagcggg
      661 ctctgccacg tccacttcgg atactatctt catcgctctt tcgcgggagg cctcgtcaat
      721 gatgaccact atggtgttcg tgcgtgaatc tacccgcatc ctgtcggcag gaaccaccaa
      781 ggccaacaga tcggccaccc cctgggcggg ggcatgttgg agcctaataa tctcgagaga
      841 ctcgtggccc gaagacaacc cagcatcggc aagcgaagcg atggcctccg gaggcaactg
      901 gacaggaacg tcaagcatcc tcagtacatc acctatggcc ctgagctctt cggctgaccc
      961 acagacgatg atggagttgg tccgctcatc cgcctccagt ttcgctccgc ctgcaacaag
     1021 ccgtgcaggg ccaataacgt cagtcgttct ggcataggtg agcgagaact tctcgatgcc
     1081 cagcggctcc aggctggttg aaacagcgcc cggcggaagg acgatcattg tagagccttc
     1141 gaccctgtat ttcacgccgg tagtgtaggc gacgagccta agcgcgtctt cgactgcaag
     1201 gccatgaagg cgaattgtga gggttccgcg taccgcgggg tcgagcacta tattcatatc
     1261 tcccagttcc gctagggcgc gaaacacgtc tgcaagttca gcatctacca ggtcaagatc
     1321 gacgcggcgc tccgaggcgc caaatgcagc ctgcccagcg aaggccaggc acaagatgag
     1381 aaccaatgct gccgccgctc gccttcccaa tcccctattt ctcatctccg ctcacctccc
     1441 agttggtaag tgaaatcagt cccgccaaac gaaatcgtta tggacttgtc gtcgatagag
     1501 cggacctctg cgccatcgaa gggcgcgtct ccgacgcgcg tcatcacgct tctcccgtcc
     1561 actgaaagca atgcaatcgc cctatcgccc gactttaaga ctccggtcag ccgcaccttg
     1621 ggcaaagccg gttcacaagg cgctgaatct tcgggctcag gcaaagctgg aatcggcgtc
     1681 ctcggttcca cggggactat ctgcaccggg tcgggaagca gagtcttgaa cgggtcccaa
     1741 cgaccgtgcc ccaccggaga aaactcctct tccactctgg accaaccata ctcgtggctc
     1801 tggtcgagca gagccgagag cctggcagca acacgccctc cggtaaccac gtcagaccca
     1861 ccaggatacc gatcgggctt tcgattcttc aggaaccaca tgtagccgga aaatgacgcg
     1921 acgagcaaca gcgcaactat gagggcggtc catctgtatt tcctcttttt agcttcggtt
     1981 tcgccgtctg gagacggcca ctcgttccgg gattctctgc taagatccag cgccaacctg
     2041 atcatcaccc ctcggcctgg cgtatgtcgt tacctgaatt gttgaagata tataagcatc
     2101 tggcgaacgg actgctcgat cctgggtttc accgccttcc cgccagagtt ctcgcccccc
     2161 tccaaaagaa aggctgtgca cattcatgac ccgctcaatg tctgcaattc cgtccagaaa
     2221 gccgattatg ttgttgtatg ttccggaaac gttcaaggtc attggtatct cgacgtactg
     2281 cccccgcggc atgccggagg cgatggtgag gctgtcaatg gcaacatggt tggacagggc
     2341 gactgactcc atatcgcgga ggacgtagtg tattctgtct tctcggggaa taaggcgctc
     2401 aaagttctcc agttgggcca tggcaagttc aagttcttct tcggacctcc cggcgcctcg
     2461 aatcttggcc aagtctactg tcagcacgga gttggcctcg tcaagacgcg ttctggcgtc
     2521 cgccagtttt cgcgcttggg gggcgaacac gtacatataa aggaggcccg ccgcagccaa
     2581 tataatcagc acgataaatg tcttggcctg cttggggttg acttccgtcg aaagagacat
     2641 gcctgtcacc tccgagcatc aagcatgcag tcgatgttga agtcgtacac ctcgtaatct
     2701 ccctcagctg ctaagcttac agaccccaca tgagcctcgc cgaacatggg cgaatcctcg
     2761 atagccagtg caaagcgggc cactgattct atggagaagg tgtctcctgc aaggctcact
     2821 ccgccatcgg ggttgattga aagagacgaa agccagatgt ccctgggaat gatgtcccga
     2881 acttctctca taacacggcc cgatgcgagg ccctcagcgc tgatagcttc agcttcatct
     2941 attcgtttcc tgatatcggc agttcgccgt tgcacctttt ccacggcctc gacatctgcc
     3001 cgaatcgctc caatttgggc ctctatccta tcgatatcct cctccaattg ctgtatgaag
     3061 aagtgcctgt aggcatagag cgctgaagcg cccactatgg caatctcaag gacgattatc
     3121 agtccgagct tggccgggct tatcccggat tcttccggtt cccgctgcgg aagcaggttg
     3181 atctttgaca tcagtcaacc acctcccgaa gggcaaggcc tatacccaca gcgaacatgg
     3241 ggccaagtct cgacagttcc tccttgttgg ctagcctctg gtttacaagg atgctcttca
     3301 gtgggtcgcc catcacagtc gcaagaccca gctcgctctc gaggtagcag tcaaggcctt
     3361 ccattactga tgcaccacct gtgagaacta ccctagtcac tccggcatcc tggcttcttc
     3421 cctcaagttg caggtcgtgg tagtcgaggg aacgtcttat atccatagca acgtctgcgg
     3481 cgacattcgc aattgcatcc gagatgtcct gttcctccct gtctgcacaa ggttcgccgc
     3541 aaccgtcgac acaaaccctt ccctttgcaa tctttattgc ctctgcttcc tcgaagctaa
     3601 ctcccaacgt ctcagcgata gcgttggtga aggtgttgcc gcctaagggg acgatccttg
     3661 tgaacctgag tctttcgcct tgggcgatga gtatatcggt agtgccggcg ccgatgtcga
     3721 catatgccgt tgctgaccta tcgttgggat ggagcgtctt atccccaggc tcatatctta
     3781 aagccctgag aacagcaaat ggctgaacat ctatggcaat gggtgtaagt ccagccagct
     3841 cgatagcttg tacatgcgat tccaccagct tccgcggggc cgcaacaagc atcacttcca
     3901 tctcggcctc atcagaaccc gagtagtcgt ctatgatctc gaaatcgact acggcctcct
     3961 caatgggcat ggggatgtgc tgttggcctt cccacttgat cgcctctctc agttcctctt
     4021 cgggcatcct aggaaacttc acgtgcctta cgactactcc ccgaccagct attgcagcga
     4081 ctacttcgcc aggcttaatc cgacccttag caaagccccg ctggataacc tccgccacca
     4141 gttcaggcga gactatctgg ccgtcccgga tggccccggg cggcgcaagc tcgatggcgg
     4201 cgcgggtcag ttcaacactg tcgccgcggc ccacaagttc cacaaccttg acgcggctgg
     4261 tgccgatatc cactccgacc atgcttctgc tcttccggga aaagaggcgc aacggcactc
     4321 atacgcaccc cctcttgaaa agactcgtat caagatactc ttcgactgta atctgaaaac
     4381 tcctaccata agtgaagaaa cccgagcagc tttgcaccga agaagacaga aatgacagcc
     4441 cctattgaca agaacggtcc gaacggaatg ggatcgcgcc tctcctgctt gcccgagaca
     4501 attcgggcta tccccaccac cgagccaatc aaggctccaa ggaaaagcgt gagcagcccc
     4561 ctatcccagc ctaagaaggc tccgaccata gccatgaact tgacgtctcc cccgcccatt
     4621 ccgcgacccc gggtaaccac gtacatgatc caaacaacgc ctcctgagac cagcaatccc
     4681 aacagcgatt gaagaggagt catgctcgga acgatgaatg atagagcgac gcctatcaca
     4741 actccgggga aagtgacgga gttcagtatg aggccagtgt tgagatcggt cccagcgcag
     4801 atgatgacca gcgagacaaa aacaaggagt gaaaggctct caaatgtgag accgtttgcc
     4861 catacgattg cagcgtaaag caacccagtc gccacctcga cgacgggata ctgaatcgag
     4921 atcggctccc cgcagtatct gcacttcccc ccaagcacca ggtagcttgc caccgggatc
     4981 aggtcgcgcg gagcaagcgg cgtgccgcag tatgggcacg aggaacccgg gttcacgata
     5041 gagcccccgg agggcaggcg gcagatgcat acattgagga agctgccgat cacgaggccc
     5101 agtatgaatg agaatagaga gatcatgtac ggcaaagaaa aacatccttt gcgattctaa
     5161 gtgggagtaa acagtcttat cagaacatat tcaatcagca tctaggataa gagacccgaa
     5221 ccacctggag caattcgggt ctcctagttg ccatcgcgaa ttcgcactag actcgtgact
     5281 gttagctggg gaacacatca gatcgtgcca actaacccaa acccgatttg gtagcggggc
     5341 acacgatgac aaatcacgaa ttgctattcg tgcccagcga gactgcaaac aaccttatgg
     5401 cttgccccat cgagcgaata ttggccgtct tccggacact tcagctcatc tgcattatca
     5461 aggtactcct tcaataccga ttgcaaagtg ctcgttccct ctgttccatc tgctgtaggg
     5521 taatcaccat gctcagcgta gtaaagccct acagcagtat caagagtcct cctatttgct
     5581 tgacatgtcg ccgccttagc cttcttgaca taattgttgt acgcaggcac tccgatcgcc
     5641 gccaacacgc cgatgatcac gataacgatc atcagctcca tcagtgtgaa gccgcgttga
     5701 tccctcttca ttgacataat cctccccctc aattctgtaa cactctgaca atctttgaag
     5761 ccacttaaac ccaaggcttg tccacccgaa ccgtccctgt gcagcgccga cgtttggcta
     5821 aagctatata tgtaaattcg tctagctgcc aacctcgcag tctctcaatt accaaaggcc
     5881 gccgcatcca caccatgcca gctggcgccc ggcaatacta atcactcgtt ccgaaaacta
     5941 cgaaatcccc atgcccttca tggtcgcaac gtataaccac actcgaggct tttccatcgt
     6001 taccgggtat tatcgtgtaa ttcccgcctg cagggcactt cagttcctca gcgttctcca
     6061 cataatctgt aaggtcagca acttcgatta cctcatctcg cggatttccc gccgagtcga
     6121 gcttgtaata tgtcgaatgc tccaaatagt aggtggccac ggcagtgctc aagattcttc
     6181 tgttggcctt gcatgcctcc gtctttgcct tgtcgactgt gcctttgtat gagggtatgc
     6241 caatcgccga cagtacgcct attatgacga tcacagtcat cagttcaacg agagtgaatc
     6301 ctcgcgcatc ctgcctcatt ccaatcacct catactcttt ggccagaatg cagctggcga
     6361 tcgaccgcag cattccaaca gtggtcgcaa tcggcctcac ctgcacccta catcccaaag
     6421 ctcgccatgt tgaacatcgg aatcatcaca gatgccacta tgagagccac aaaaagcccg
     6481 attatcaatg tcataatggg ctctatcgcc gtggtaactc gttggacctc atattcgact
     6541 tcttggtcga agaaatcagc agttttgttc aacatgccgt cgagattacc cgtttcctcg
     6601 ccaactgcga ccatgtgcgc aagcattggc gggaatattt gcgattgtct gagaggtctg
     6661 gcaagtccgg cgccatcacg aacagatatc ttggacgctt ccactgtctc cctgactacg
     6721 gcattatctg acaacctttc aagaatgtca agagattgca acatgggcac gccactttgg
     6781 gttaacatgg cgaaagtcct tgcaaactgg gcagtctggc tcttccggat aatggggccc
     6841 gaaatcttca gccggattgc agtatggtcc caccagctct tccccgctcc cttcacatag
     6901 cttacaaacc aaacaatggc gactattggg ataccgaaat gcaaatacca cgcccgttgc
     6961 acgctctgcc catacccgaa tacggctcga gtaacccacg gcagctccat atcgaacccc
     7021 tcgaacatgt cgatgaaagt gggcaacacg aaagccatca gaataacgac gaccaccaaa
     7081 gctactgcca caagtattat cggataaacc agcgcgttct taagctggct tctaatcttc
     7141 acctgttgct catagtagtt ggccacgcgg ttcatgacta tgtccaaggt tcccgacgct
     7201 tcccccgcct ccaccatgtg cacgaaaagc ggcgggaagc acccagggtg tttcgctaag
     7261 gatgcagcaa aagtctcccc cgactccacg ctgcgcctca cctcgttgat aagctcggcg
     7321 atacggggat cctctatctg ttgtgagagg atggccaacg tttgaaccag gggaattccc
     7381 gcagccacca tggtggacat ctgacgggaa ataagcgaaa gctgcatcgt atcaaggctc
     7441 gtcgcagcct gcaccttctt gcctgaggcc gccgacgcgt cagctgagaa caccgatcgt
     7501 ttagcttcag tgatcatcac gggaacatag ccctccgacc gaaggcgttc ggcaactgtt
     7561 tccggattct ccgcctccat ggtgccttca accacctgcc cgctcatgtc cctcccgcga
     7621 tacgaccaag aaagactagc cacttcccct cacccccttg cctaaagcct cataatcggc
     7681 tgagcaaact ggcctagccg cctcatctca tcagggttcg tcgcatggct cagcgcatca
     7741 tcgtacgtga tgatcccgcg tttcgccaaa tcgacgaggt acatatcaag gctcaccatc
     7801 ccctgctttg cgcctgtttg gagcaccgaa taaatctgat gagtcttccc ctcgcgtata
     7861 aggttgcgaa cagcgggatt tgccacgagg acttccaccg ccacaatcct tccctgtccg
     7921 tctgcgcgtg gcaacagaat ctgggatact ataccctgca gcaccgccgc cagctgaacc
     7981 ctgacctgtt gttgctggtg aggaggatac tggtctatga tcctgtccac agtctgaacc
     8041 gtatcgttgg tgtgcaacgt ggcaaacacc aaatgcccag tttcggcagc ggtaagagct
     8101 atagaagtcg tttccagatc ccgcatctcc cccacgagga taacatcagg atcctccctg
     8161 agggcagccc tgagcgccgc cgcaaaagac ttggtgtccg tccccacttc cctctggttg
     8221 atcatgctct ttcggtgctt gtgaaggtac tctatggggt cctccaaggt gatcacgtgg
     8281 cacgcccgct ccgaattgat tatgtcaatg agagaggcca gggtcgtcga ctttccgctt
     8341 cccgtgggac cggtaaccag caccatcccc ctggtcttgt cggcaaacgt cttcaccaca
     8401 ggcggaaggc caagttcctc caaggacctg atccttgttg gaatgaccct gaacgctgcc
     8461 cccacggagc cacgctgcgt ataagcgttc accctgaacc t
//
LOCUS       DAOSAI010000058         8444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5484_length_8444_cov_4.215759, whole genome shotgun sequence.
ACCESSION   DAOSAI010000058 DAOSAI010000000
VERSION     DAOSAI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5484_length_8444_cov_4.215759"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLM83_03375"
     CDS             <1..334
                     /locus_tag="PLM83_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOK70481.1"
                     /translation="GKIDNIDAVLVSSCAEAGDPTATAILRNAFEYLGIVVANIVNLL
                     NVEMVVIGGGVSQIGGMLFDTVRRVVDLRAFPFMAKDAKIVPAALGVDAGMLGAACYA
                     MEKQSGED"
     gene            374..1303
                     /locus_tag="PLM83_03380"
     CDS             374..1303
                     /locus_tag="PLM83_03380"
                     /inference="COORDINATES: protein motif:HMM:NF021500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HOK70482.1"
                     /translation="MKRLCLALIIVFLPALAVMMWSARTSAAHFPAGVGQRLIWEADD
                     PEGDDYGPGTYVYPTNPAFAPFSGLFDLIGFRVSCDDENVYFDTKLAKISNTWNAPEG
                     FSHQLINIYIDTTSGAGRTDTLREGAFVSFAPEYAWDVNIKIMGWGGSRVFFASDNPA
                     SAGIIEGLSVEALPDGNTIRATVSRQVIGEPLDSWKYYVLVSSQDGYGLDNFRPVMGE
                     AGPWVFGGGSDLNINPNVIDMLAPESGVYSQENQLGSWDLSERRYARIVPANAQLHRR
                     HLTVRTIILIIAVGGAAALAYIYFRGRSNVGRG"
     gene            1318..3849
                     /locus_tag="PLM83_03385"
     CDS             1318..3849
                     /locus_tag="PLM83_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70483.1"
                     /translation="MYAKSTSRICAFAIALFMLMFGVPAQSSLGWPIWEWDWDTEPAE
                     PGEPHVPAEAMLSEEDPSEDDTERILDVVFAFGLMGNVSPDSAGQASATCERLLDVLL
                     SYPDMRFSVGISGTLLTGLAWEGSRAVQLLRDGIGRGVIEPLGATYSEGVLASMEPWD
                     AQLAVSLGSEAVSSFLGEKPTGFWNPQGVWRQDVIVPVAIGGYTHTILEDTIVEASGL
                     GMLPPNAPMRVSWGGREAVLFQADTDFGRFVDNAIRTGSTRPVIDYLQQLYEGDKQDS
                     AVVVYAKELMVTEDKTEWEGLSLLLRALADEEWIRVTTLRERVEAGPPPGSVIQIADG
                     VPRGFQKALSDEGFSDWAAFNSSRNLEALRVLYSDIRARIHSVEAAVRKAEASGKDTS
                     SAQKLLDDAKMVFISSQYGFGRPGSAGAVSGDANWEMGRAAYVSALAAWQCVNPTVSA
                     YQEDINRDGVEEVIMITDTDMFVLSPIGAKLLYWYDLENGEELVGSAVQQYVGEIFVN
                     ESMAGGGRQRALVDSISGPAAVGIDLSSAEFGVEIGEDSPSATFVFKDGGTAIEKSVA
                     AKEGALEVDYTVRSRGRISFAVSNGLCPDAHWTRIGGKDSLVYYDPNGAVASSLRQGK
                     SFGLMNVVTGTVVRLTVGGDRDDGVPLSRVSSTLDSFVRRLDLEYKFAMAPGASANFG
                     FELQRSKAWPSPSAVLWIQEVDNEIVVGLPTYTRACAIRQFVDGYVTDTPMVAFSSTL
                     KKARKGQEKSALEVGAPLPKGRYDLVAVLVTDRKALIENAGSYVSGTGKHEFFPEVRD
                     SVVEITPGYARVGGTVVMWVAIGVGAGIVVAGVIVYLRRRKVSGA"
     gene            4328..5524
                     /locus_tag="PLM83_03390"
     CDS             4328..5524
                     /locus_tag="PLM83_03390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOK70484.1"
                     /translation="MRILMFSWEYPPRVIGGLARHVCELARAMARCAEVHVITCSAEG
                     AQSMDRDRGVWVHRVDPVGPCTDDFVTWAMQLSYGMLARAACLVSEGGAFDIVHAHDW
                     MTSFAGVGMKRAYATPLVATIHATEWGRNGGLHNATQRRISDIEWFLCYEAWRVIACS
                     RHMQAELAGIFGIPADKLRIIPNGVYSEQFQDADADIARRMYGLSEEDSVILFVGRLV
                     NEKGVQVLIEAMPKILSYQPTAVLVIAGQGPCEGDLRELAANMGLGGKVKMVGFVSDE
                     ARNALYRLARVAVVPSLYEPFGITALEAMAAKTPLVSSDIGGLSEIVRHGENGWKCYA
                     GNANSLADAILHVLYSPELARQMAETAYRDVQTIYDWRAIADRTLRVYEEVLREARHA
                     EEAMVR"
     gene            5521..7977
                     /locus_tag="PLM83_03395"
     CDS             5521..7977
                     /locus_tag="PLM83_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOK70485.1"
                     /translation="MKAVIMAGGKGTRLRPLTCSMPKPMVPVVNRPMMEHVVDLLVAH
                     DFRDLACTLCYMPEAIQEHFGDGSSVGCTMTYSVENTPLGTAGSVRALGDFLDDTFLV
                     ISGDALTDIDLTSAVTFHRERGAAVTIVLTRVATPLEYGVVIVDGDARITRFLEKPSW
                     GEVFSDTVNTGIYVLEPEIMQRVDPSCEFDFSKNLFPLLLAEGYPMYGYIADGYWCDV
                     GNLDQYRKTSQDILEGKVRVKIPGDLVDEGIWVSEGAEIGNIAALRPPVVIGAGAKIE
                     AGAAVGEYSVVGPSCIITEGASVRRSILWTGCFIGQNAEVHGAILGSRVSAKAGVLIQ
                     EGAVIGSGCSMGERAQVRPGVKIWPDKTLDGGAQISASLVWGASWSKRLFGRLGVTGL
                     ANIEITPDFAARLGAAYGSCLSSGVVAVSCDAHPASDMIKRALASGILSSGISVADLG
                     SGSTAVTRYAVPAIGASGGVHVRVSPTGGNASLIEFLDASGINIDKATERKVENAFLA
                     EDFKRANSDKVGSTITTEGMVGRYLGAISSSIDADTVRRRGLKVVGGYDSEYVGALID
                     AFCNACGCDLHHIEISNTAWAPSEVADAVAVTEADLGFLLDRNAERLALVDETGSNIS
                     NEEMLALICVALLTSGRETTMAVPVTASSAIERLAAAFRGYVVRTQANPRAMMEKAIE
                     ARVRMGEASLPHFQPVMDGVFALAVILEYLAREKTTLSAFAQIAPRSYMTKQVVDCPW
                     EDKGRVMRRLIEETDGEEVQLIDGIKVYSDHGWTLILPDSEEPLFHVYSEAHSQEIAE
                     EIAGIYVSRIAETISDAGAV"
     gene            complement(8151..>8444)
                     /locus_tag="PLM83_03400"
     CDS             complement(8151..>8444)
                     /locus_tag="PLM83_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOK70486.1"
                     /translation="LARSYPHPQPELTRRALAQAARELLLAQSSDWAFIMKAGTCIDY
                     AKKRFETHTAHFLGLYDDIKSSNIDPEWLSELEEQSNLFPNIDYMVYAGRATM"
BASE COUNT         1885 a         1914 c         2616 g         2029 t
ORIGIN      
        1 tggcaagatc gacaatatcg atgcggtact cgtatcctct tgtgctgaag caggagatcc
       61 tacggcgact gcaatattgc gcaacgcttt cgaatatctt ggtattgtgg tcgcgaatat
      121 agtgaacttg ctcaacgtgg agatggtggt tatcggcggg ggggtatccc agataggcgg
      181 catgcttttc gacacggtca gacgggtagt ggatttgcgc gcttttccgt tcatggctaa
      241 agatgccaag atcgttcctg ctgctttagg ggtagacgcg gggatgttgg gcgcggcgtg
      301 ctacgccatg gagaagcagt ctggggaaga ttgagcccag agcggtagga tatgattatc
      361 cggggggaag acagtgaaaa gactatgcct ggcacttatc attgtgttct tgccggcgct
      421 ggcggtgatg atgtggtcag cgcgcacgtc cgcggcgcat ttcccggcag gggtggggca
      481 gaggcttatc tgggaagctg atgatccaga aggcgatgat tatggaccgg gaacatatgt
      541 ttatcccacc aacccggcat tcgccccgtt ctccggatta ttcgacctaa tagggttcag
      601 ggtttcctgc gatgatgaga atgtttactt cgacaccaaa ctggccaaga tttctaacac
      661 ttggaacgcg cccgagggct tcagtcatca gctcatcaat atatatatcg acacaacctc
      721 tggcgctggc cgaaccgata ctctaaggga gggagccttc gtctctttcg cgccggagta
      781 tgcatgggat gtgaatatca agataatggg gtggggcggc agccgggtat tcttcgcatc
      841 tgataatccg gcatcggctg gtatcatcga aggattgtca gttgaggctc ttcccgatgg
      901 aaatactata cgcgccaccg tgtcgcgaca agtgatcgga gaacctcttg actcctggaa
      961 atactacgtg ctcgtttctt ctcaggatgg ctatgggctc gacaacttca ggccggtcat
     1021 gggggaagcg ggtccgtggg tttttggtgg aggttctgat ctcaacatca accccaacgt
     1081 aatagacatg ctcgccccgg agagtggtgt atattcccaa gaaaatcaac taggttcatg
     1141 ggatctgtct gagcgtcgat acgccaggat tgtgccggcc aatgcccagc tgcacaggag
     1201 gcacctaacc gtcagaacca tcatcctcat tatcgctgtg ggcggcgctg cggcgctggc
     1261 atacatctac tttaggggca ggagcaacgt tggaaggggt taagggggag cacgcctatg
     1321 tacgctaagt ctacttcaag gatctgtgca ttcgcaatcg ccttgtttat gctgatgttt
     1381 ggtgtgcctg cgcagtccag tctaggctgg ccgatctggg aatgggactg ggatacggag
     1441 ccggcagaac ctggcgaacc gcatgtgcct gctgaggcca tgctcagtga ggaggatccc
     1501 tctgaggatg acacagagcg gatcttggat gttgtatttg cttttggact catggggaac
     1561 gtatctcctg acagcgccgg ccaagcatct gcgacctgtg aaaggttgct cgacgtcctc
     1621 ctttcatatc ctgatatgag gttttcggtt ggaatatccg ggacgcttct gacagggttg
     1681 gcatgggaag gtagtcgcgc agtccagctg ttgcgtgatg ggatcggcag aggagtcata
     1741 gagccgcttg gcgccacata ttccgaggga gtcttggcat ccatggaacc ctgggatgct
     1801 cagcttgcgg tatcattggg gtctgaggct gtaagcagct tcctgggcga aaagcccacc
     1861 gggttttgga atcctcaagg agtgtggaga caggacgtga ttgtgcctgt ggccatcggc
     1921 ggatacaccc atacgatatt ggaggatacg atagtcgaag ccagtggact tggaatgctg
     1981 cctccaaatg ctccaatgcg cgtctcatgg ggcggaaggg aagcagtgct ctttcaggca
     2041 gatactgatt tcggtcggtt tgttgacaat gcgattcgca ccggatccac acggccggtc
     2101 atcgactatc ttcagcaact ttatgaaggg gacaagcagg actctgcggt ggtggtgtac
     2161 gccaaggagc tcatggtgac tgaggacaag actgaatggg aagggctctc gcttcttctc
     2221 agagccttgg cggatgagga atggatcagg gtgactacat tgagagaaag agtggaagct
     2281 ggtcctccgc caggcagcgt gattcagata gcagatgggg tgccccgggg atttcagaag
     2341 gctctgtcgg atgagggatt ttcggattgg gcggcgttca attcttctcg taatcttgag
     2401 gcgttgcgcg ttctctattc tgacattaga gccagaattc attctgtaga agcggctgtc
     2461 agaaaagctg aagcatccgg gaaagacacg tcttcggctc aaaagctgct cgacgatgca
     2521 aagatggtct ttatatcatc ccagtacggg tttggtcgcc ctggttctgc aggggcggtt
     2581 tccggggatg ccaactggga gatgggcagg gccgcgtacg tgtcagctct cgccgcgtgg
     2641 cagtgcgtaa accccacagt ttcagcttat caagaggaca tcaaccgcga tggtgtagaa
     2701 gaggttatca tgatcaccga tacagacatg ttcgttctct cgcccatagg cgcaaaactt
     2761 ctatattggt atgatttaga gaacggcgag gagcttgtag gaagcgcggt acagcagtat
     2821 gttggggaga tatttgttaa cgaatccatg gctggcggcg gccgtcaaag agcgctggtt
     2881 gattcgattt caggtcctgc tgcggtcggg attgatctct cttcagccga atttggtgtc
     2941 gagataggtg aggattctcc cagcgcgacg tttgttttca aggatggcgg caccgcaatt
     3001 gaaaaatcgg tggcggcaaa agaaggggct ctcgaagtcg actacaccgt cagatcgcgt
     3061 ggtcgcatca gctttgctgt atcgaatggc ttatgcccgg atgcgcattg gactaggata
     3121 ggcggtaagg attccctcgt ttattatgat cccaatggag cagtggcaag ttctcttagg
     3181 caggggaaga gttttggcct gatgaacgtg gttaccggaa ctgtcgtgag actcactgtc
     3241 ggtggtgatc gcgacgatgg tgtgccccta tcccgggtct cttccacgtt ggacagcttt
     3301 gtcagacgtc tcgatcttga gtataaattc gccatggcgc caggtgcttc tgcgaatttc
     3361 ggctttgaac ttcagcggag caaggcatgg ccttctccat ctgcggtcct ttggattcag
     3421 gaagtagaca atgagatagt tgtcgggctt cccacctata cgagggcatg cgcgattcgt
     3481 cagtttgtcg acggatatgt tactgatacg ccaatggtgg ctttttcctc gacgctcaag
     3541 aaggcaagga aagggcagga gaagtcagcg ttggaagttg gggcgccctt gccaaaaggc
     3601 agatatgatc tggttgcagt cttggttaca gacagaaagg ctttgatcga gaatgcaggt
     3661 tcttatgtca gtggcacggg caagcacgag ttctttcccg aagtgcgcga ttctgtggtg
     3721 gagataacgc ctggttatgc cagagtcggc gggacagttg tgatgtgggt tgccattggc
     3781 gtaggtgccg gaatagtggt ggcgggtgta atagtctacc ttcgaagacg caaggtttcc
     3841 ggcgcataat tcatccttca tagccaatta taagactgcg gtttgaagtt cagaccgcag
     3901 tcttgtggtt cgccgaacat gttttgcgag ccgaagttgg cctgtcaggt gggcaaccct
     3961 gtgctgagtg gaaaagcctg aaaacgggat aacctgaggg ttcgggcgga cagacttaac
     4021 aaggggagcc ctatatcatc cataacgcta caaatcataa acaaagaggg atgcaaggac
     4081 aggaagacat ggtgcatagc ggcagatgag gccgtggtag tcattgatcc taaagctgat
     4141 aggaacggag gagccgggcc tgttcagtag agcaccaaag cctggcgggg tagattccct
     4201 tcgagctaga gatgcctcac aaaatacgag gtcggcatct tgcatctctt caatgaagca
     4261 aagcgaccaa gggttgccta cctggcgcca ccttactcga tctatgtcct tcaaagaggg
     4321 gtgttttatg cgtatactga tgtttagctg ggagtatccg cctcgtgtta ttgggggact
     4381 ggcccgccat gtatgtgagc tcgctcgcgc tatggcgcgt tgcgccgagg ttcacgtaat
     4441 cacgtgcagt gcggaagggg cgcaatccat ggaccgcgat cgcggagtgt gggtacacag
     4501 agttgatccc gtaggtccct gcacagatga ttttgtaaca tgggcaatgc aattgtccta
     4561 tggaatgctt gcccgggctg cgtgccttgt ttccgagggc ggcgcgttcg atatagtgca
     4621 cgcacacgac tggatgacgt cgtttgccgg tgtcggcatg aaaagagcct atgccacgcc
     4681 tctcgtagcc acgattcatg ctactgagtg gggaaggaat ggcggccttc ataacgccac
     4741 tcaacgacgg atatccgaca tagaatggtt cctctgttat gaagcgtggc gagtcattgc
     4801 atgttcacgc catatgcagg ctgaacttgc gggcatattc ggcattcccg ccgataaact
     4861 gcgaatcatt cctaacggtg tttattccga gcagtttcag gatgctgatg ccgatattgc
     4921 gcggcggatg tatgggcttt cggaagaaga cagcgtgatt ctctttgtcg ggcggcttgt
     4981 gaatgagaag ggcgttcagg tattgatcga ggccatgccg aagatcctga gctaccagcc
     5041 tactgctgtg ttggtcattg caggtcaggg tccatgtgag ggcgatcttc gcgagcttgc
     5101 ggccaacatg gggttgggcg gcaaggttaa aatggtgggt tttgtctcag atgaggcaag
     5161 gaatgcgttg tacaggcttg ccagggttgc tgtcgtgccg agcttgtatg agccgttcgg
     5221 gataactgct ttggaggcaa tggcagcaaa aacacctctt gtctcgtctg atatcggtgg
     5281 cctttctgaa atagtcaggc acggcgaaaa cggatggaaa tgttacgccg gcaatgcgaa
     5341 ctcgcttgcc gatgcgattt tgcacgtgct ttacagtccc gagcttgcca ggcaaatggc
     5401 agagacggct tatagagacg tgcagactat ctatgactgg cgcgccatag ctgatcgtac
     5461 cctcagggtg tatgaagaag tgctcagaga ggccagacat gcggaggagg ccatggtcag
     5521 gtgaaagctg tgataatggc aggaggaaaa ggaactaggt tgaggccttt gacgtgcagc
     5581 atgcccaagc cgatggttcc ggttgtaaat aggcccatga tggagcacgt cgtggatcta
     5641 cttgtcgccc atgatttccg agatctggca tgtaccctct gttacatgcc agaggcaata
     5701 caggaacatt ttggggatgg gtcgtctgtg gggtgcacaa tgacctattc agtagagaat
     5761 acccctctag gcactgctgg aagcgtgaga gctcttgggg attttctcga cgatactttt
     5821 ttggtcataa gtggcgatgc tctcactgac atcgatctca cttccgcggt tacctttcac
     5881 agagagaggg gggcggcagt taccatcgtt ttgacacggg ttgcaactcc attggagtat
     5941 ggagtcgtaa tagtggacgg cgatgcgcga atcaccaggt ttcttgagaa accgagctgg
     6001 ggcgaggtgt tcagcgacac tgtaaacacc ggcatttatg ttctggaacc tgaaattatg
     6061 cagagggtgg acccatcctg cgagtttgat ttcagcaaga atctttttcc gcttctactt
     6121 gctgaaggat atccgatgta tggctacata gctgatggat actggtgcga tgtgggcaac
     6181 ttggatcagt atcggaagac gtcccaggat atcttggaag gaaaggtccg agtgaagatc
     6241 cccggagacc tcgtcgatga gggaatctgg gttagcgagg gcgctgagat agggaacatc
     6301 gctgccttgc gtccgcctgt agtgatagga gcgggagcaa agatcgaagc tggcgcggca
     6361 gtcggggaat actcggttgt tgggccgtct tgtatcataa cagaaggggc atctgtccga
     6421 agatcgatac tgtggacagg ttgtttcatc ggacaaaatg cggaggtgca tggcgcaatc
     6481 cttggaagta gggtgagcgc caaagcaggt gtactaatac aggaaggagc ggttataggt
     6541 tccggctgct ccatgggtga acgagcacaa gtgcgccccg gcgtgaagat atggcctgac
     6601 aagacattag atggcggcgc acagatcagc gctagcctag tgtggggagc ctcctggtcg
     6661 aagcgcctgt ttgggcgtct tggtgtgacc ggacttgcta acatcgagat cactccggac
     6721 tttgcggccc ggctaggcgc tgcctatggc tcgtgtttat ccagcggggt agtggcggtg
     6781 tcctgcgatg ctcatcccgc gtccgacatg ataaagcggg cattggcgtc agggatcctt
     6841 tcctcaggca tctcagtggc cgacttaggc tcaggctcaa ccgctgttac cagatatgcg
     6901 gttcctgcta ttggggctag tgggggagtg catgtgcgcg tgtctccaac tggcgggaac
     6961 gcgagtctca tagaattcct ggatgctagc gggatcaata tagacaaggc aactgaaagg
     7021 aaagtagaaa atgcctttct ggcagaggac tttaaacgcg cgaattctga caaggtgggc
     7081 agcactatca ccaccgaggg gatggtaggt agatatctcg gtgcgatcag ctcgtcgata
     7141 gatgctgaca cggttcggag gcgaggcctg aaggtggtag ggggctatga ctcggagtac
     7201 gtgggagcac ttatcgacgc cttctgcaat gcttgcggat gtgatctgca ccatatagag
     7261 atctccaata ccgcctgggc accgtcagag gtggcggatg cagtggccgt gacagaggcg
     7321 gacctcggtt ttctgcttga ccgaaatgca gaaaggcttg cgcttgtcga cgaaacgggt
     7381 tcgaatatct caaacgaaga gatgctggcg ctcatatgtg tggccctcct tacatcgggt
     7441 cgtgagacta ccatggctgt gccggttaca gcgtcctcag cgatagagcg gctggctgct
     7501 gcgttcagag gatatgtagt ccgaacccaa gcgaacccca gagccatgat ggaaaaagcc
     7561 atcgaggcgc gagtaagaat gggagaggcc agtttgcctc actttcagcc tgtaatggat
     7621 ggggtttttg ctcttgcggt gatccttgag tatcttgcga gggagaagac tactctttcg
     7681 gcgtttgcgc aaattgcgcc gagatcatat atgaccaaac aagttgtgga ttgtccctgg
     7741 gaggacaaag gcagggtgat gaggaggttg atagaggaga ctgatggtga ggaggttcag
     7801 ctgatcgacg gaataaaggt ctacagtgac catggctgga ctttgattct tccagactcc
     7861 gaagagcctt tgttccacgt gtacagtgag gcgcatagcc aggagattgc ggaggaaata
     7921 gccgggatct atgtcagtag gattgctgaa accatatcag atgcgggtgc ggtctaaaga
     7981 gcgcgcaacg cgcaattatg cgtagcagcc tgattaaccc gacattgata ttgaagcagg
     8041 cccacaagct tgagggcgat ggggcttgaa aagttcaaat gtgagtgttg cttcatgaaa
     8101 gtatggcgcg cggcgagcgc ccgcggcttc cctctgtcga gcacctgggc ttacatagtt
     8161 gctctgcctg cgtacaccat gtagtctatg ttgggaaaca ggttactctg ttcttccagc
     8221 tcagaaagcc attccggatc tatgttcgat gattttatgt catcatacag ccccagaaag
     8281 tgtgcggtat gcgtttcaaa ccgctttttt gcataatcga tgcaggttcc ggctttcatg
     8341 atgaaagccc agtcggagct ctgagccaac agcagttcgc gtgcggcttg agctagggcg
     8401 cgccgcgtca actccggttg gggatgtggg tatgagcggg ccag
//
LOCUS       DAOSAI010000059         8372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5548_length_8372_cov_4.172899, whole genome shotgun sequence.
ACCESSION   DAOSAI010000059 DAOSAI010000000
VERSION     DAOSAI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5548_length_8372_cov_4.172899"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1349)
                     /locus_tag="PLM83_03405"
     CDS             complement(252..1349)
                     /locus_tag="PLM83_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK70487.1"
                     /translation="MTHQFAVPFSDPKPDFEGFRQVLEGKRDANRVHYAELFADREIV
                     EAILADYMGLTPVPSPEDDFEEYWRQQIEYWYKMGYDYIRVAGGLEIPRAKHRIAEDT
                     AALSRGERGWIEQGIGEIASWADFEEYPWQTVEDMDFSAYEFVAKNLPEGMKMLVCPS
                     SGVFEISSEYMLGFEGMSMMLYEDPELVEAVFLRVGQMLFDFYRNAVTIDNVAGIFQG
                     DDLGFKTSTFLSPTHLRKLVFPWHKRYAELAHERGQVYWLHSCGNLQNVIDDLIDDVG
                     IDAIHSFQDEIMPVADFKDEYGHRVGVLGGIDMDKLCRLDEADLRSYVRTTIDRCMPR
                     GWALGSGNSIANYVPVRSFLIMMDEGAKWRG"
     gene            complement(1511..2716)
                     /locus_tag="PLM83_03410"
     CDS             complement(1511..2716)
                     /locus_tag="PLM83_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK70488.1"
                     /translation="MKVKRGLLAVVCGALILSLAIIPSMAADPLVVWCEPQKMGTIEP
                     LAAQWSKQTGIPVKVEPVGVLETANKVQLAGPMGKGPDVFCCLSGQLGMLVVTGTVAP
                     VDPKVFDLSNFMKVGVDAATFDGKLYGVPYDISSVAMIYNKKIWPEAPKTFDEMIARS
                     RDLQKKGMFGILWPLENFYFTYAIMAGYGGYVFAPTKSGWNTKDIGLANPGSVKAIKL
                     LKTLRDEGLIPVGTDHVVAPAKFSEGKAAAIIDGSWALPGIKQAGIDYGVAPIPRLDN
                     GTYPAPFVSLKWWHVSSYSQHKAEAAKLIAYLTTKDAMYKSFKAAEGIPPRLDVLEMP
                     DVKVMPEVAGYGAQANFGSILPDIPEMNPVWVPMNNAIEIALRGDKSVEAALADAVKI
                     IEQNIAELK"
     gene            complement(2834..3700)
                     /locus_tag="PLM83_03415"
     CDS             complement(2834..3700)
                     /locus_tag="PLM83_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK70489.1"
                     /translation="MSANTATGKNARKAKRTVGTVLIYAGLIIACIIVLYPLLWVLSC
                     SFKPTAGLVGSSLIPSNPTLANYRKIFTDPNVNFSRWFFNTLKISVVSALLTLVLTTP
                     AAYAFSRMRFPGRRQTLIGFMICQMFPGFMAIVALYTLLGWAGLIDTHLGLIILYAGG
                     AIPFSIWLLKGYFDSVPVEIEESAMIDGATKFQAFTRILLPLAKPILYVVGLINFLGP
                     YAEYLLAQVVITSGSKWTLAMGMRSLTVSQFATDWPIFSAVSVLTAVPIVIVFLSAEK
                     YIVSGLTRGAIK"
     gene            complement(3716..5011)
                     /locus_tag="PLM83_03420"
     CDS             complement(3716..5011)
                     /locus_tag="PLM83_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK70490.1"
                     /translation="MATANPHPQVAGILSALLAGAGQAYNGQRTKGIAYMAVEAVFLV
                     LLFTVLRDPLYGLVTLNPVGRFVDSRHILLAGIIAAFVVAIYAWFHIANIVDAVRTAS
                     SIRKRHALPDDMQRIHPEYRTPAGTIAPYLYIAPSLLVAFFVIVIPLAFGIALAFTNY
                     NLYHCPPAGRFDWIGLENFRRLLKPGSLWKDQLDMVLVWNIVYALLGTVLAFLCGLAL
                     SLMLENRHIRFKGAFRTILMLPWAVPATVSIMVFFGLFNTTFGPINEILKNIGLKAVP
                     WFQDRTWARVSVVLTHVWISTPFNLSVISAALQSIPEEIYEAATVDGASPWQSFSKIT
                     FPLLMSVVAPILVLTLAGNFNNFGIIYLLTGGGPAVAGSKGAGATDILMTWVYDLGFQ
                     QLQWSIASALAVLVFIFVVVFSLINFRLSGVLRQLREEE"
     gene            complement(5197..6294)
                     /locus_tag="PLM83_03425"
     CDS             complement(5197..6294)
                     /locus_tag="PLM83_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK70491.1"
                     /translation="MFHDISYYKPDLHDCINRVRDFFSLEPGAPQALIYTFPSVLTLR
                     GPALNQYDFEHDMQSYLDDYLEYQAQLMEIRLGIEDDWILSVVPYMGIGEFSAFVAGE
                     IEFGEDTSWAAPVINEKSDLETLCLDPNNKWFRKLNEATRYMVRKIAPYRIPLGRGYY
                     SPLDLAWALRGEAIYMDFYEDPQFVHNLMEFALSATEWFARAQAAEIFAPDVVHELSA
                     WHCGPNRVAVSEDISSLISPSHYAQYGAPYTQRLFDSFGIGEVHCHSAGPHVVPEFLK
                     LKRVRQIQIVADPNTRRPVEILADLMDKNPELFGKSPDVPVIAVDASPQEAVDYYDLS
                     RQTRIVFSVVTENAEEARHAVDIFRAKQKQC"
     gene            complement(6508..8190)
                     /locus_tag="PLM83_03430"
     CDS             complement(6508..8190)
                     /locus_tag="PLM83_03430"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOK70492.1"
                     /translation="MEKILMMTGGGFHPFHLCSSIAKDFLEQTGIFTVEITEDRDKFL
                     DLSNYSAVIVYTQGGELTAKQENGLLTYVRSGGGIVGIHSAADSWTNNKGYMEMIGAQ
                     FREHGPMTDITVTYSKEADAVIPRADKSFVIHDEFYMLKPHAEEALKPFMYGSWHQAQ
                     EILGYVRDYGKGKVLYTALGHDEKAFNVPQFQELIIKGLMYVIGKISDETIRFGIVGY
                     GPLYGMGKYHADSINNTTGLITTAICDKDPARLRAAKNELGDIPTFTDASEMAQSGLV
                     DAAVVAVPPNAHAEVVLALIESGLHVVSEKPFAITTAEVDQMIEAAQCRGVVLSVFHN
                     RHWDSDIVTLRQIMDLGLIGEVYSVECNMVSYQMPYPGWRSQKEVSGGLLYDMGVHQF
                     EKAFQIAAYNWGQDCRSRKAFVYGNRLKKAWHHVNVEDYWRAYVKFESGLEMQVVQSN
                     LCAAPKPLWVVLGTRGAAVIESWDWEKPAIVNTYVNNRLTRVEYPIIPPCSIPDYFDR
                     LAEKYYRNLADHVLMDLPLIITPEWARNPVAAIELSEKASLENRVLEAEFIF"
BASE COUNT         1931 a         2165 c         2173 g         2103 t
ORIGIN      
        1 tttctcctgt gacattttcg ctgccgttct ctgtatttct attatgccga aaacatgttt
       61 gcctcctcaa atcgagtctc tccggactcg tttatttggt gagaaatatg cttacacaac
      121 tgtagcacaa aggcatgccc gcagcaagcg acatgatgtg gaaatcgata aagttatcga
      181 gctaatcgca gatctccaag aaggtgtgtt gcttcagttt ctgctgcccg atggcgtcgc
      241 gctcaagtgc cttatcctct ccatttcgcc ccctcgtcca tcattataag gaagctgcga
      301 acaggcacat aattggctat ggaattgcct gagccgagag cccaacctct gggcatgcac
      361 ctgtctatcg tggttcgcac gtagcttctg aggtctgctt cgtccaaacg gcacagtttg
      421 tccatgtcta ttcccccgag gacccccacc cggtgcccat actcgtcctt gaagtcggca
      481 acaggcatga tttcgtcttg gaaggagtgt attgcatcta tacctacatc gtcgatgagg
      541 tcgtctatca cgttctgcaa gtttccgcag gaatggagcc agtatacctg cccgcgttcg
      601 tgggccagct cggcatagcg cttgtgccag gggaagacga gcttgcgcag atgtgtgggg
      661 gagaggaatg tcgaggtctt aaaccccagg tcatctcctt ggaagatgcc agcgacattg
      721 tctattgtga ctgcgttgcg gtagaaatcg aacagcatct gtccgacacg aagaaacacg
      781 gcttctacga gctccggatc ttcatacagc atcatgctca tgccttcgaa tcccagcatg
      841 tattctgacg atatctcgaa tacaccgctc gaggggcaga ctaacatctt catcccttcg
      901 ggcaagtttt ttgcaacgaa ttcgtaggcc gaaaagtcca tgtcctccac agtttgccag
      961 ggatactcct caaagtcggc ccaggaggct atttcaccga ttccctgttc gatccagccc
     1021 cgttctcctc tcgaaagggc tgcagtgtct tcggctattc ggtgcttggc ccgtggtatc
     1081 tctaggccgc cagcgacacg aatgtagtca taccccatct tataccaata ctctatctgc
     1141 tggcgccaat actcttcaaa gtcgtcttct ggggagggga cgggggtgag ccccatgtag
     1201 tccgcgagga ttgcctcaac tatctcgcgg tcggcgaaga gttcagcata gtgaactcgg
     1261 tttgcgtccc gctttccctc aagcacttgt cggaatcctt caaaatcggg tttgggatcg
     1321 gaaaagggca ctgcaaattg gtgggtcatg ttcgtatccc tcctcagacg aaatgggcag
     1381 atgggcccgc cctctggcgt ctgtctggat ctggcgccgg gcgggccccg tcagctcata
     1441 ttgaatcatg tctctagcaa ccagttgctt tatactgcat cttgcggcgc gtggccgccg
     1501 acttttactc ctacttgagc tcggctatgt tctgctcgat gatctttact gcatcagcaa
     1561 gggctgcctc tacactcttg tctccgcgca gggcgatctc gattgcgttg ttcatcggta
     1621 cccatacagg attcatttcc ggtatgtccg gcaggatcga tccgaagttc gcctgcgcgc
     1681 catagcctgc gacttcaggc atcaccttga catcgggcat ttcgagaacg tcgagtcgcg
     1741 gagggatgcc ctcggcggcc ttgaaggact tgtacattgc gtccttcgtt gtaagataag
     1801 caatgagctt cgcagcctct gctttgtgct ggctgtagga gctcacgtgc caccacttga
     1861 ggctcacaaa cggcgccgga tacgtgccat tgtcgagcct cggaatgggc gccactccat
     1921 aatcgatgcc agcctgcttg attccgggca gagcccagga gccgtcgatg atggcggctg
     1981 cctttccctc actgaacttg gcaggagcaa ccacgtggtc ggtgccgaca gggatgagcc
     2041 cttcgtctct gagcgtcttg agcagcttga tcgctttcac agatcctggg ttggcaagtc
     2101 cgatatcctt cgtgttccat cctgatttag tgggggcaaa gacatacccg ccatagcccg
     2161 ccattatcgc ataagtgaag tagaagttct ccaggggcca caagattccg aacatgccct
     2221 tcttttgcaa gtcgcgggac ctggcgatca tctcgtcaaa ggttttgggt gcttcaggcc
     2281 agatcttttt gttgtagatc attgccacac tggaaatgtc gtacggcacg ccgtacagtt
     2341 tgccatcgaa ggtggctgca tcaacgccta ctttcatgaa gttggacaga tcgaagacct
     2401 tgggatcgac tggggccacg gtccctgtga ccactaacat gcccagctgg ccagaaagac
     2461 agcagaacac atctggccct ttgcccatcg gcccggccaa ctgcactttg ttggcggttt
     2521 cgagaacgcc tacgggttca accttcacgg gaatgcctgt ctgtttgctc cactgggctg
     2581 caagaggctc tatggttccc attttttgtg gctcacacca tactacaagg ggatctgccg
     2641 ccatcgacgg gatgatggcg agagatagaa tcaatgcgcc acaaacgacg gcgagaagcc
     2701 cacgcttaac cttcattcta cctttcctcc tttatggttt tggatctcaa agatatttga
     2761 gccaacaatt cgcgtccctg cttgggggaa gcaggtcaat ctgattcgat acactgcgtc
     2821 ggctatgtca tgcctacttt atcgctcccc gggtgaggcc tgagacgatg tatttctctg
     2881 ccgagaggaa cacgatgacg atcgggactg ccgtcaacac cgacactgcg gagaatattg
     2941 gccagtccgt agcgaattgc gataccgtta ggctcctcat tcccattgcc agtgtccact
     3001 tgcttcctga agttatcacc acctgtgcaa gcagatactc agcgtatggt ccgaggaaat
     3061 tgatgagtcc gacgacgtag aggattggtt tggccaacgg caagagaatt ctggtgaacg
     3121 cctgaaactt cgtggctccg tcgatcatcg cagactcctc aatttccacg ggtactgagt
     3181 caaaatagcc tttgagcagc cagatggaga agggtattgc acccccagcg taaaggatta
     3241 tcaatcccag atgggtatct attagcccag cccagcccag aagggtgtag agggcgacaa
     3301 tcgccatgaa cccaggaaac atttggcata tcatgaaccc tatcagggtt tggcgtctgc
     3361 ccggaaagcg catgcgcgaa aaggcatacg cggcaggtgt tgtcagaacg agcgtcaaca
     3421 atgcgctgac aacggatatc ttgagcgtgt tgaaaaacca gcggctgaag ttgacgttgg
     3481 ggtcggtgaa tatcttcctg tagtttgcca atgtgggatt agatgggatc aagcttgatc
     3541 ccacaagccc ggctgtgggt ttgaatgagc acgacagaac ccagagaagg gggtagagca
     3601 ctatgatgca ggctatgatg agccctgcat atatcagaac cgtgcccaca gtgcgctttg
     3661 cctttctcgc gttcttccct gtagctgtgt ttgcggacat ctgattcgcc tcccctcatt
     3721 cttcctctct gagctggcgc agcacgccgg agagtctgaa attgatcaag ctgaagacaa
     3781 ctacgaatat gaagaccaat actgcgagtg cggacgcaat cgaccattgg agttgctgga
     3841 aaccaagatc gtagacccat gtcatgagta tgtcggtggc tcctgcgcct ttgcttcccg
     3901 ccactgcagg cccgccgccg gtcaaaaggt atatgatgcc gaagttgtta aagttgcctg
     3961 ccagtgtgag aactagtatc ggagcaacta ccgacatgag cagggggaag gttatcttgc
     4021 taaacgattg ccaaggtgat gctccgtcga ccgtggctgc ctcgtaaatc tcctccggga
     4081 tgctttggag tgcggctgat atcaccgaca ggttaaatgg agtcgatatc catacatggg
     4141 tcagcaccac gctcacccga gcccatgtgc gatcttgaaa ccacggcacg gccttgagcc
     4201 cgatgttttt caggatttcg tttatcgggc caaacgtcgt attgaacagg ccgaagaaca
     4261 ccatgatcga tactgtggcg ggaactgccc acggaagcat cagtatggtg cgaaaggcgc
     4321 ctttgaatcg tatgtgtcgg ttctcgagca tgagggatag ggctagcccg cagaggaacg
     4381 cgagaaccgt gcccaatagc gcgtagacta tgttccatac aaggaccatg tcgagctggt
     4441 ctttccacag agatccgggc ttgagcagtc tgcgaaaatt ctcgaggcca atccaatcga
     4501 atctgcctgc gggagggcaa tggtacagat tgtaatttgt gaaagcgagc gcaatcccga
     4561 acgcaagggg tatcacgatt acgaagaagg ccaccaagag cgagggcgcg atgtacaaat
     4621 agggcgctat cgtgccagcg ggggtccggt attcggggtg tatgcgctgc atgtcgtcgg
     4681 ggagcgcatg gcgttttctg atggatgatg cggttctcac agcgtctacg atatttgcta
     4741 tgtgaaacca tgcgtagata gcaacaacga atgctgcaat gatccctgcc agaaggatgt
     4801 gcctcgaatc aacgaacctt cctacagggt tgagagttac gagcccatag agagggtcgc
     4861 gtagtacggt gaaaaggagc acgagaaaga cggcctccac cgccatatag gcaataccct
     4921 tcgtgcgctg tccgttgtat gcctgcccag cgccggccag cagggccgag aggatcccgg
     4981 ccacttgagg atgtgggtta gctgtggcca ttgagaagtc cctccgtatg ctttgaactt
     5041 gaaatgacag ggcaagctga taagttgaac ggcgttgggg cttgggcaac ggtgtttcac
     5101 atgggctatg gttgtagatg cctttgcctt ttgtggacct tgtgtgttgt ggctgttcct
     5161 ttcgacgatg atgccccggt aacctctaat aaccgatcaa cattgcttct gtttcgccct
     5221 gaatatgtca accgcatgtc gcgcttcttc tgcgttttca gttaccacag aaaaaacgat
     5281 gcgggtttgt cgagacagat cgtagtagtc gacggcctcc tgtggcgagg cgtcaactgc
     5341 tatcacagga acatcaggtg atttgccgaa taattcagga ttcttatcca tcaggtcagc
     5401 gaggatttcc accgggcgtc gggtgtttgg atcggcgacg atctggatct gccgcactct
     5461 cttgagcttc agaaactcgg ggactacatg tggcccggct gaatggcaat gtacctcgcc
     5521 tatgccaaag gagtcaaaga gcctctgcgt gtaaggcgcc ccatattgtg cgtaatggct
     5581 tggagagatg aggctcgaaa tatcttcgga aactgcaacc ctattgggtc cgcaatgcca
     5641 tgccgaaagc tcatggacca cgtccggcgc aaagatctcg gctgcttgag cccttgcaaa
     5701 ccattctgtt gctgatagag caaattccat caagttgtgg acgaactggg ggtcttcgta
     5761 gaaatccatg tagatggctt cgcccctgag ggcccaggca aggtcgaggg gggaatagta
     5821 gccgcggcca agcggaattc ggtaaggcgc tatcttgcga accatgtaac gggtagcctc
     5881 gttaagcttt ctgaaccact tattgttagg atcaaggcaa agcgtttcaa gatcagactt
     5941 ttcgttgata acaggagcag cccagctcgt atcctcgcca aactctatct cgccggctac
     6001 gaaggcgctg aactccccga tgcccatgta ggggacgacc gacaatatcc agtcgtcttc
     6061 gatcccgagg cggatctcca ttagctgtgc ctgatactcg agataatcgt cgagatatga
     6121 ttgcatgtcg tgctcgaagt cgtactggtt tagggcaggt ccgcgaaggg tcagcactga
     6181 ggggaaggtg tatatcagcg cctggggtgc tccaggctcc aggctgaaaa aatcgcgaac
     6241 ccggtttatg cagtcatgga ggtcgggttt gtagtacgag atgtcgtgaa acatgtgaac
     6301 accccgcttg taatggctta agatcgtgct caggaagaca tgtctcctta tcggttactt
     6361 agtagtcatg acaccaacta ctataataat attcgtatac atccccgatg tcaatatcgc
     6421 caattaggag gcagcgtctg taacctgagc atgtatgtgg aatcatgagc cgaaatggaa
     6481 gaatatccat gaaaatctgt attggcctta gaaaatgaat tccgcttcga gcaccctgtt
     6541 ttccagagaa gctttctctg aaagctcaat agctgcaacg ggatttcttg cccactcagg
     6601 agtgatgatc agaggcagat ccataagcac atggtctgca aggtttcgat agtatttttc
     6661 agccagcctg tcgaagtaat caggaatact gcaaggcgga ataatcggat attcaactct
     6721 cgtaagcctg ttgttcacgt atgtattgac gattgcaggt ttctcccaat cccaactttc
     6781 tatgacggct gctccccggg ttccaagtac aacccacaag ggctttggtg ccgcgcagag
     6841 atttgattga acaacctgca tctccagacc ggattcgaat tttacatatg ctctccagta
     6901 gtcttcgacg ttaacatgat gccaagcttt cttgagacgg ttgccataaa caaaagcttt
     6961 gcgacttcgg cagtcttgtc cccaattgta cgcagctatc tggaacgctt tttcaaattg
     7021 gtgaactccc atatcgtaca gcaaaccccc ggaaacttcc ttctgacttc gccaacctgg
     7081 atatggcatt tgatatgaga ccatattgca ctcaacggag tacacttcgc cgatcaagcc
     7141 taggtccata atctgcctga gcgtgacgat atccgaatcc cagtggcggt tatggaagac
     7201 ggagagaact acacccctgc attgggcagc ttctatcatc tgatcaactt cagctgttgt
     7261 tatcgcaaaa ggtttctccg atactacatg gagcccgctc tctatcaaag ccaacacaac
     7321 ctcggcatgc gcattggggg ggactgcaac tactgcggca tcaacaaggc cgctttgggc
     7381 catttccgag gcatccgtaa atgtggggat atcaccgagc tcatttttgg ccgctcgtag
     7441 gcgggcaggg tctttatcgc atatagctgt tgttatcaac cctgtagtat tgttgatgct
     7501 atctgcatga tacttgccca ttccatatag gggtccataa ccaacaatgc caaaacgaat
     7561 tgtttcgtcg cttatcttgc caataacata cataagaccc ttgataatca gttcttgaaa
     7621 ctgtggaacg ttgaaagctt tctcatcatg tcctagtgca gtataaagca ctttcccttt
     7681 tccgtagtcg cgaacatagc ccaggatctc ttgtgcttga tgccagctac catacatgaa
     7741 tggctttagc gcttcttcgg catgcggctt aagcatgtag aattcgtcat gaatgacaaa
     7801 gcttttatca gctctaggga taacggcatc agcttctttg ctgtaagtga cggtgatatc
     7861 cgtcatcgga ccatgctctc taaactgagc gccgatcatt tccatatagc ctttattatt
     7921 ggtccaagaa tcggcagccg agtggatacc tacgatgccg ccaccacttc gaacataagt
     7981 gagcaggccg ttttcctgtt tggcggttag ctcaccgccc tgagtataga ctatgacggc
     8041 actgtaattt gacaaatcta ggaacttatc tcgatcctca gtgatttcaa ctgtaaatat
     8101 ccctgtttgc tccaaaaagt cttttgctat gctgctgcac aggtgaaacg ggtgaaatcc
     8161 tccaccggtc atcataagta ttttttccat atagcatact ccttccgaag atactgcggc
     8221 cttctgctag gactaagcgg gtagttaggc ccgatgcgcc ggcatctatt tttgctgctc
     8281 tgacggattt acagatgcca gcgcatcggg cgggttgtgt caaattctgt tcggtaactg
     8341 cccggctcta tttgagctca gctatgtctt gt
//
LOCUS       DAOSAI010000060         8104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5767_length_8104_cov_3.388123, whole genome shotgun sequence.
ACCESSION   DAOSAI010000060 DAOSAI010000000
VERSION     DAOSAI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5767_length_8104_cov_3.388123"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLM83_03435"
     CDS             complement(<1..691)
                     /locus_tag="PLM83_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70493.1"
                     /translation="MDGLSPVNAVTIDISKDETITLRHMDFGALSDGLKLYDEDISAR
                     EFTVRLLHPYLIEPEISLDSMSKWKDNVILHVASEWYTKTSGFKDSDEISLTTFEDFR
                     SLLANQAKKTAEHLRGAMASPFHNRGAIYEALAQNMFGPINVVNTSLLEARRLLVDTA
                     QQTTNAYVQALVNQPSALSQLERSITSVSSCLRNSISTTISNLQSLQSILDNSRKASQ
                     GADALKEHDYGF"
     gene            complement(782..2818)
                     /gene="brxL"
                     /locus_tag="PLM83_03440"
     CDS             complement(782..2818)
                     /gene="brxL"
                     /locus_tag="PLM83_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HOK70494.1"
                     /translation="MDELDKLLNQHFAGRVVRKDLTKQIKEGANVPVYVLEYLLGMYC
                     ATDDEEAIEAGISTVKNIIADNFVRPDEAEKIKSKIRELGQYTIIDNITVKLNEKRDV
                     YEAEFSNLGIRGVDVPVNYVKRYDKLLGGGIWCLAKMEYYYDEFDRNRSPFVLVNLNP
                     IQLPTMDIDEILEARKYFTKEQWIDVMMRSIGLEPSQLDYNVKWHMLLRLVPLVENNY
                     NLCELGPRSTGKSYVYKEISPNSILVSGGQTTVANLFYNMNTRQVGLVGLWDCVAFDE
                     VAGIKFKDQDGIQIMKDYMASGSFARGKEEKHANASMVFVGNINQSVEAILKTSHLFE
                     PFPDAMANDTAFFDRMHYYLPGWEVPKMRPEHFTDNYGFIVDYISEFFREMRKRPSYA
                     DCIDHYYKLGRDLNQRDVIAVRKTVSGMAKLIYPDGNFGKEETEELLQYALVGRRRVK
                     EQLKKIGGMEFYDVRFSYVDKETLEENYVSVPEQGGGKLIPEGTMKPGHIFTAGRGSN
                     GNMGVFKIETEAIAGAGRFECAGLASGGRDARENVKLAFKYFEVNAKHISGAIAVQRE
                     DYMMHVIDLQGVGVTSELSLAAFIALCSVALGRPVQPEMAVLGSMTIGGTITKVEELA
                     NLMQVCFDAGAKRILLPMASAVDIATVPAELFSKFQLAFYQSPEDAVVKAVAAD"
     gene            complement(2832..5390)
                     /gene="pglZ"
                     /locus_tag="PLM83_03445"
     CDS             complement(2832..5390)
                     /gene="pglZ"
                     /locus_tag="PLM83_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HOK70495.1"
                     /translation="MKLSEVEKQLNSIFSSPPKSGSNRHIVFWYDPDAEFADDIDAIE
                     LQEVRLWKLTGKNNYVTKRLLEAGDTQSNFLIYCPGPRPEPRKNWLLDIELYSERFTA
                     DRTEAIMRELGIEDPSLRDAIKNSSKFFNSQERISKFKALGLTEHTEEAIEIGILSVL
                     AKEKVADSGGVFKALLREGLDAESNETYQAIEKMGCEALLVKHAAREFGYEDTSFDLE
                     KFAITLFVSALEHQSDVDMPKTWEPFISKEKANCYILIDSLGKQSDAREDYRCLAAHV
                     ANKLNFLGYVEEWDPGDYQDCGIFREFDMEIISRIAKGIQAGIDDFEYWRELIASRRT
                     TRWFPDFKHTYDAFDDAIDIFELKAKWDAIPTHLDAQSLFHAYTERYYKMDRAYRRFY
                     TSYDAANKPDILNEVKARVEAVYTNWFLQELGLAWSRRVEEELADGWSMVGVPQQDSF
                     FHVIKEHLERDARVFVILSDALRYEVAQELAERIQSEIPGTTKLEAVQGILPSRTSVG
                     MAAALPHTSLAIAEDGSGVVDGEHTEGTEQRQALLAKAGYPAAVFTFEELTDLTTEQM
                     RARLDGIKIVYIYHNRIDATGDKAASERNVFQACEESIDEIIDAIVALRNKVSATNIY
                     ITSDHGFIYTRDPLEESDKITQMAAGGKKPLGRRFTLAPAPVSDKGVLSIKSPHKSPN
                     GEDLYVITPKGYIRFKIQGPGINYTHGGASLQEIVVPRIWHRSERTARRGANKPEIEL
                     RSTVNRITNRFFTLEFFQKQPAVGPVQPRTVTARFVDASGSTISDEAIIVADSTSADP
                     NERLIRTRFTLTGDRFDKNAEYYLVLEDQEETVEKILLKRRFIIDLGISAAFEF"
     gene            complement(5387..>8104)
                     /gene="pglX"
                     /locus_tag="PLM83_03450"
     CDS             complement(5387..>8104)
                     /gene="pglX"
                     /locus_tag="PLM83_03450"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HOK70496.1"
                     /translation="DEAEQDPKVIAELDKIARKNIDPAEITFIDPACGSGHILVYAFD
                     LLYEMYLEAGYPETSIAAMILEKNLYGLDIDERAVQLASFALLMRARSKDPQILSKGI
                     LPNVIVIEESNNISPEMIDLLVSSEASHDEQKQQRHELECLLQVFEDATEYGSLIRVP
                     ELNLEWLPEATAWAIERASSDRADDTATLDGPTIEEQAKAISRLARQAKVMSEKYDVV
                     VTNPPYMYTRNMNKTLRDYLKAAYKSCGSDLFASFMDQCMDSTKPRGFTAMITQHSWM
                     FLSSLRALREAIVKDKTIYSMLHLGARTFESLGGEVVQSTSFVLRNVHVPSYYGTYIR
                     LVEFDTPEEKINKLDNAANHYTFSTDEAARIPGSPIAYWASRNVIRAFEEGTRLGEIA
                     PPRQGMATSDNDRFLRLWHEVKETDVGFDMASRTEAKESGCRWFPYNKGGEFRKWYGN
                     NDYLVNWANDGVEIKGAVISAYPYLNGKYQWVVKNEEFYFLPSITWTFVSSSRFGARS
                     SPEGFLFDVGGSSAFPDEEDKLYVTAFLCSKLAFEFLKMLNPTLNFQVGNIRDLPLLF
                     PSSPDVRSRIESLAAECIDIAKRDWDSFETSWDFVRHPFLVYGAGADTIEEAYARWEK
                     FADGEFERMRANEEEINRHFIDIYRLGDEMTPEVSDDDVTINRADRDRDVRSFISYAV
                     GCIFGRYSLDVDGLAYAGGEFEWDNYETFIPDTDNIIPVLDDAYFPNDIVRRFTEFVG
                     ACFGKKHLDKNLDWIAETLGKRSGETAADTIRRYFINDFYRDHTQTYKKRPIYWMFSS
                     GKQKGFNALVYMHRYQRDTVARLRTKYLLELESRLQAAARSLRDPETSSAPRGQTLRE
                     LEKLERQLAEIKEYDEKVHHLADRMIEIDLDDGVVVNYAKFEDVLEAIR"
BASE COUNT         1617 a         2125 c         2086 g         2276 t
ORIGIN      
        1 cgaatccgta atcatgttct ttaagcgcgt ccgcaccttg tgatgctttt ctcgaattgt
       61 ctagaattga ctgcagagac tgcaagttac ttatggtcgt actgatgcta tttcttaggc
      121 atgacgatac agacgtgatg ctcctctcca gttgagataa cgctgaaggt tgattaacta
      181 aggcctgcac gtatgcgttg gttgtttgct gagctgtgtc tacaagtaag cgtctcgcct
      241 caagtaagga tgtgtttact acgttgattg ggccaaacat gttttgtgcg agcgcctcat
      301 atatagctcc gcgattatgg aacggtgagg ccatggcccc acggaggtgc tcagctgttt
      361 tctttgcttg attagccagc agactcctaa aatcttcaaa ggtggttaac gatatttcat
      421 cgctatcttt gaatccagat gtttttgtgt accattcgga ggcaacgtgc agtattacat
      481 tgtctttcca cttggacatg gagtcaagag agatctccgg ctctattagg tatggatgta
      541 gcagcctaac cgtgaactct cttgccgata tatcttcatc atacaattta aggccatccg
      601 ataacgcacc gaagtccatg tgcctcaatg taatggtctc atctttgctg atgtcgatag
      661 tgacagcatt tactggcgac aaaccgtcca agtcgagaaa cttctgagtc atgttaccat
      721 tgtccctatc tatctcaacg cacccccata acatgagttg acccggcgaa cattttcgct
      781 gctaatccgc cgccactgcc ttgacgactg catcttctgg gctttggtaa aatgccagct
      841 ggaacttgct gaatagttca gctgggactg ttgctatgtc aacggcggag gccatgggca
      901 acaggattct ctttgctccg gcatcgaagc aaacttgcat gaggttagcc agctcctcca
      961 ctttcgtgat ggtgccgcca atggtcatgg accccaacac tgccatctca ggctggactg
     1021 gtctgcccag ggctactgaa cagagggcta tgaatgctgc gagtgagagc tctgatgtaa
     1081 ctcctactcc ctggagatcg attacgtgca tcatgtagtc ctctcgctga acagcgatgg
     1141 cgccgcttat gtgcttggcg ttgacttcga agtatttgaa agccagtttg acgttttcgc
     1201 gagcgtctcg ccctccggat gcgagacctg cgcactcgaa tcttccggcg cctgctatgg
     1261 cttctgtctc tatcttgaag actcccatat tgccattgct gccacggccg gctgtgaaga
     1321 tatgtccggg cttcatggtc ccttcgggta tgagcttgcc tccgccctgt tcgggaactg
     1381 atacatagtt ctcttccagg gtctctttat ctacatatga aaagcggacg tcgtagaact
     1441 ccatgccgcc gatcttcttg agctgttcct ttacgcgtct tctaccgacc agggcatatt
     1501 gtagtaattc ctcggtctct tccttgccga agttgccatc tgggtatatc agtttcgcca
     1561 ttcctgaaac ggtcttcctg actgctatca catcgcgttg gttcaggtcg cgccccaact
     1621 tgtagtagtg atctatgcaa tcggcatagg atgggcgctt acgcatttcc cggaagaact
     1681 ctgatatgta gtcgacgata aacccgtagt tgtccgtaaa gtgttctggg cgcatcttgg
     1741 gaacctccca tcccgggagg tagtagtgca ttctgtcgaa gaaggcggtg tcgttggcca
     1801 tggcatcggg gaagggctcg aaaagatgcg atgtctttaa gatggcctcg acgctctggt
     1861 tgatgttgcc cacgaatacc atggaggcgt tagcgtgttt ctcctccttg ccacgggcga
     1921 aggagccgct tgccatgtaa tccttcatta tctgaatgcc gtcctggtcc ttgaatttga
     1981 tgccggctac ttcgtcaaag gccacacagt cccatagtcc cactagcccc acttgtctgg
     2041 tattcatgtt gtagaagaga ttggctacgg ttgtctgtcc gcctgagaca agtattgagt
     2101 tcggggatat ctctttgtaa acgtacgatt tacccgtgct tcgggggcca agttcacaca
     2161 ggttatagtt gttttcaact aagggcacga ggcgcaggag catgtgccat ttcacattat
     2221 aatccagttg cgacggttcg aggccgatag agcgcatcat cacatcgatc cactgctctt
     2281 tggtgaagta ctttcgcgct tcgaggattt catcgatatc catcgtcggc agttgaatcg
     2341 ggttgaggtt caccaagacg aatggactcc tgttgcggtc gaactcgtcg tagtaatact
     2401 ccattttggc aaggcaccag atgccgccgc ccaggagctt gtcgtaacgt tttacgtagt
     2461 tcacagggac gtcgacgccg cggatgccga ggtttgaaaa ctcagcctcg tagacgtctc
     2521 gtttttcgtt gagctttact gtgatgttgt ctatgattgt gtactggcca agttcacgga
     2581 tcttcgattt gatcttctcg gcctcgtccg ggcgcacgaa gttgtcggcg atgatgtttt
     2641 tcacggtgct gatgcccgcc tctatggctt cttcgtcatc cgtggcgcag tacatgccca
     2701 gcagatattc gaggacgtag actgggacgt ttgcgccctc tttgatctgc tttgtaaggt
     2761 cttttcgcac tacgcgaccg gcgaaatgtt ggttgagcag tttatcgagt tcatccacgt
     2821 ctgatcgcct ctcagaattc aaatgctgcg cttatgccaa ggtctattat gaacctgcgc
     2881 ttgagcagaa tcttttccac tgtctcctct tgatcctcta gcaccaggta gtattcggca
     2941 ttcttgtcga acctgtcgcc tgtaagcgtg aacctggttc tgatgagcct ctcattggga
     3001 tccgctgagg tgctgtcggc cactattatg gcttcgtctg atatcgtgga ccctgatgcg
     3061 tcgacgaacc tcgcggtgac ggtgcgtggt tgcaccggtc ccacggcggg ctgcttttgg
     3121 aagaactcaa gggtgaagaa tctgtttgtg atcctgttca ctgtggatct gagttcaatc
     3181 tctggtttgt ttgccccgcg tcgtgcggtg cgttcgcttc tgtgccatat tcgcggcacg
     3241 acgatctcct gaaggcttgc gccgccatgg gtgtagttga tccctggccc ctgtatctta
     3301 aatcttatat acccttttgg ggtgatgaca tagagatcct ccccattggg gctcttgtgg
     3361 ggcgacttta tggaaagtac tcctttatcg gaaactggag cgggtgccag tgtgaatctt
     3421 cgtcctagcg gcttcttgcc tcccgctgcc atctgagtta tcttatcgct ttcctccaat
     3481 ggatcacggg tgtagatgaa gccgtggtcg gaggtgatat agatgttggt agcgctcact
     3541 ttgtttctca atgcgacaat ggcgtcgatt atctcgtcga tcgactcttc gcatgcctgg
     3601 aagacgttgc gttcggatgc ggccttgtct ccggtggcgt ctatgcggtt gtggtagatg
     3661 tagactattt ttatgccatc gaggcgggct cgcatctgct ctgtcgtcag atctgtcaac
     3721 tcttcaaatg tgaagactgc agccgggtat cctgcttttg ccagaagagc ttgtctctgc
     3781 tctgttcctt cggtatgctc tccgtctacc acaccagagc cgtcttcggc tattgcgaga
     3841 ctggtgtggg ggagggctgc ggccattccc acggacgtcc ttgaaggtag gattccctga
     3901 actgcttcga gctttgttgt tccggggatt tcgctttgga tgcgttcagc cagctcttga
     3961 gcaacctcgt aacggagtgc gtcggatagg atgacaaaga ctcgcgcatc cctttcgaga
     4021 tgctccttta tcacatggaa gaatgaatcc tgctggggca ctccaaccat tgaccatcca
     4081 tccgcaagct cctcttcgac tcgtctgctc catgcaaggc ctagctcctg aagaaaccag
     4141 ttcgtgtaga cggcttccac tcgggctttc acttcgttta gaatatccgg cttgttggcg
     4201 gcatcgtagg aggtgtagaa tcgtcggtat gctcggtcca ttttgtaata gcgttcagtg
     4261 tatgcgtgga acaagctctg agcatcgagg tgcgttggaa tcgcgtccca tttggctttc
     4321 aactcgaaga tgtcgatggc atcgtcgaat gcgtcgtatg tgtgcttgaa atctgggaac
     4381 cagcgtgttg tgcgcctact ggcgatcagt tcacgccagt attcgaagtc atcgattccg
     4441 gcctgaatcc cttttgctat gcggctgatg atctccatgt cgaattcccg aaagattccg
     4501 cagtcttgat agtctccggg atcccactct tccacatagc ccaagaagtt tagcttgttg
     4561 gccacgtgcg cagccaagca tcgatagtct tcccgtgcgt cgctctgctt tcccaggcta
     4621 tcgattaaga tatagcagtt ggctttttcc ttggagataa agggctccca cgtcttcggc
     4681 atgtcgacat cggactggtg ctccagggcc gagacgaaga gggttatggc gaacttctct
     4741 agatcgaacg atgtatcttc gtagccgaac tcgcgcgccg cgtgtttgac cagaagcgcc
     4801 tcgcaaccca tcttttctat ggcctgatat gtctcgttgc tttctgcgtc caatccctct
     4861 cgcaacagtg ccttgaagac tccgccactg tctgcgacct tctcctttgc gagcacggac
     4921 agtattccta tctctattgc ttcctcggtg tgctcggtta ggcccaaggc tttgaatttg
     4981 gatatccgtt cctggctgtt gaagaacttg ctggagtttt tgatggcatc gcgcaacgag
     5041 gggtcctcta tccccagctc gcgcatgatc gcttctgtcc tatccgccgt aaaccgctct
     5101 gaatacagtt cgatgtcgag cagccagttc ttgcgcggct caggtcttgg cccgggacag
     5161 tagataagga agttgctttg ggtatcgcct gcttccaata ggcgttttgt gacgtagttg
     5221 ttcttgccgg taagcttcca caggcgtacc tcctgaagct cgatggcgtc tatgtcatcg
     5281 gcgaattcgg catcaggatc gtaccagaag acgatatgtc tgttgctgcc gcttttgggc
     5341 ggactggaga atatggagtt gagctgcttt tctacctctg aaagtttcac cttatcgcct
     5401 ccaggacgtc ctcgaatttg gcgtagttga ctacgactcc gtcgtccagg tctatctcga
     5461 tcatgcggtc ggccagatga tgaacctttt catcgtactc tttgatctcg gcaagctgcc
     5521 gttcgagctt ctccagttcc ctcaatgttt gtccgcgggg tgctgaactt gtttcggggt
     5581 cgcgcaggct gcgagctgcc gcctgcaggc ggctttcaag ctccagcagg tacttggttc
     5641 tcagcctggc cactgtatcc cgctgatacc ggtgcatgta caccaatgca ttgaacccct
     5701 tctgcttccc agaagagaac atccagtaga ttgggcgttt cttgtacgtt tgggtatggt
     5761 cgcggtagaa atcgttgatg aagtaccggc gaatcgtgtc tgcagccgtc tctcccgatc
     5821 tcttgcccaa ggtctccgcg atccagtcga ggttcttatc gagatgcttt ttgccaaagc
     5881 aggcccccac gaactccgtg aatcttcgta ctatgtcgtt ggggaagtag gcatcgtcga
     5941 gcaccgggat gatgttgtcg gtgtcgggga tgaacgtctc ataattgtcc cactcgaatt
     6001 cgcctcctgc ataggcaagc ccatccacgt ccagcgagta tctgccgaat atgcacccaa
     6061 ccgcgtacga gatgaatgaa cgcacgtccc gatccctgtc agctctgttg atggttacgt
     6121 catcatctga aacctccggg gtcatctcat ccccaagcct gtagatatcg atgaagtgcc
     6181 tgtttatctc ctcttcattc gccctcatcc gctcgaactc gccatcagcg aacttctccc
     6241 accgcgcgta agcttcttct atcgtgtcag ctccagcccc atacaccagg aacggatgcc
     6301 tcacaaaatc ccacgaggtt tcgaacgaat cccagtccct ctttgcgatg tcgatgcact
     6361 ccgctgcgag ggattcgatg cgggatcgga catcggggga ggaggggaat aggaggggga
     6421 ggtctctaat attgccaacc tggaaattca gggttgggtt caacatcttg agaaattcaa
     6481 atgccagttt cgagcacaaa aacgcggtga catagagctt gtcttcttcg tcaggaaatg
     6541 cagagcttcc gcctacatca aagaggaagc cttcaggact tgaacgtgct ccgaagcggc
     6601 tggagcttac gaaggtccag gtgatggagg ggaggaaata gaactcctca ttcttgacga
     6661 cccactgata cttgccgttt agatacggat aggcactgat gacggcaccc ttgatctcta
     6721 cgccatcatt tgcccagttg acgaggtaat cgttgttccc ataccatttt ctgaactctc
     6781 cacccttgtt gtatgggaac catctacagc cactttcttt ggcctctgtt cgtgacgcca
     6841 tgtcaaagcc aacatcggtt tccttgactt catgccatag ccggagaaag cgatcattgt
     6901 cagatgtggc cattccttgc cggggtggag cgatctcgcc aagtcgtgtt ccttcttcaa
     6961 atgccctgat cacatttcta ctggcccagt aggctatggg ggagccgggg attctcgcgg
     7021 cctcatcagt actgaatgta tagtgatttg ccgcattgtc gagtttgtta attttctctt
     7081 caggagtgtc gaattcaacc aggcgtatgt aagttccgta gtagctagga acatgaacgt
     7141 ttctgaggac gaaagaagtt gactggacaa cttctcctcc gaggctttcg aaggtgcggg
     7201 cgcctaagtg caacatggag tatatcgtct tatctttgac tattgcttct cgcaaggccc
     7261 tcaagctgga caagaacatc catgaatgct gtgtgatcat tgcagtgaag ccacgtggtt
     7321 tggtcgagtc catgcattgg tccatgaagc tagcgaacag atcggatccg caggacttat
     7381 atgcagcttt cagataatct cttagcgtct tgttcatgtt gcgagtgtac atatatggcg
     7441 gattcgtaac cactacatca tacttctcac tcatcacctt ggcctgcctt gccagcctcg
     7501 atatggcctt cgcctgttcc tctattgtcg gcccatccag cgtggctgta tcgtccgctc
     7561 tgtcgctgga ggctctctct atggcccagg ccgtcgcctc gggcagccac tcgagattca
     7621 gctctgggac gcggatgagc gagccatatt ccgttgcatc ttcgaatacc tggagcagac
     7681 actcgagttc atgtcgctgc tgcttttgct catcatggct tgcttcggag ctcaccagga
     7741 ggtctatcat ctctggcgat atgttgttgc tctcctctat gacaatcaca ttgggaagaa
     7801 tgcctttgga cagaatctgt ggatccttcg acctggctcg catcaggagc gcgaacgaag
     7861 cgagttggac tgcgcgttca tctatgtcca gcccatacag gttcttctcc aggatcatgg
     7921 ctgcgatgct ggtttcgggg tacccagcct ccagatacat ctcatagagc aagtcgaatg
     7981 cgtagaccag tatatgtccg cttccgcagg cagggtcgat gaaggtgatc tcggcagggt
     8041 cgatgttctt tctcgctatc ttgtccagtt ctgctatcac ctttggatcc tgctcagcct
     8101 cgtc
//
LOCUS       DAOSAI010000061         8091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5782_length_8091_cov_3.607018, whole genome shotgun sequence.
ACCESSION   DAOSAI010000061 DAOSAI010000000
VERSION     DAOSAI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5782_length_8091_cov_3.607018"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLM83_03455"
     CDS             <1..592
                     /locus_tag="PLM83_03455"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOK70497.1"
                     /translation="VPARVGHRVPVQPRQVVESMDVNQGSLVIGYWTGVNMSDPDYPA
                     ILMANGIYGGYAHSKLFQNVREGESLAYSVYSGIDQNKGVMNVWAGIAPEAMDKAVEI
                     IEEQLDDVRSGRISDDEMDKTRRAITRDIRMMGDSPGAQIDYAIVSRINGVGEKPDEL
                     LRRIEAVSMDDIVRVASKIELDTIYFLRNEEDEAGA"
     gene            594..1880
                     /locus_tag="PLM83_03460"
     CDS             594..1880
                     /locus_tag="PLM83_03460"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOK70498.1"
                     /translation="MEIARSELLREEVSTTRLDCGLEVCVQRKAGYAKKYALIGTRYG
                     SLDTKFRRIDTGETVDVPDGLAHFLEHKLFAEESGSIDDVFAELGAYSNAFTDYTMTA
                     YLFSCVDNFGPCLDTLLDFVMRPHFTEENVENEKGIIEQEIKRDEDSPAWQAMYGMLK
                     GMYQCFPVRLEVAGTVDSIRSIDVDILTRCYNTFYHPSNMTLLVVGDVDPNEVADRAS
                     AHLDDKLHGPVPAVERLYPHEPREVGMKRVERRMPVSEPLVCFGFKDNDVGYEGAQLL
                     RKTVCMEMLLYTLIGRSSALYNELYEAGLLDHTFDWNFDCEKDYSFVIFEGKSNSPDA
                     LVDRVCKGINDSIAAGIDRELFERARRSFIGSALRAFNSLEFIAYNFLAYHFRKMRLL
                     DRVGVAESISLDEVMDLGKQLFDPEMLVVSVVNPMQ"
     gene            1907..2575
                     /locus_tag="PLM83_03465"
     CDS             1907..2575
                     /locus_tag="PLM83_03465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOK70499.1"
                     /translation="MTKLLLIRHGETMFNRARRLQGCKDVPLSDLGTRQADACANYIA
                     SLNRGTITALYSSPLARAYMTASAIGKAVGLDVVTDDRLKEIDVGALEGMTWDEVQLA
                     YPDFMVEHRTDPIGTRYPGGESVADVSARARDLADGFVCDHGDETIVVVGHAIVLKAL
                     ICRVLNLDVANHLRLTVGNASLSIASVSRVDDDVKGKVLLLNDRHFLEALGLDAPRKE
                     GHEE"
     gene            2897..3040
                     /locus_tag="PLM83_03470"
     CDS             2897..3040
                     /locus_tag="PLM83_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70500.1"
                     /translation="MAYNGEPVRCGGGVSFFIGEHVGDIGLQAAFYDCAFSVEEAQES
                     AEK"
     gene            3081..4241
                     /locus_tag="PLM83_03475"
     CDS             3081..4241
                     /locus_tag="PLM83_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOK70501.1"
                     /translation="MCGVSESSRPRAREIGIHTGIFSPGEFNAITDVDGVMVGHRTVV
                     HGEGELVPGKGPARTGVTAIRPHGGNVFREKAPAAAYVFNGYGKSVGIHQINETGVLE
                     TPILLTNTLNVGLVADALIEWSVERNGDIGISTSTVNPVVGEVNDGYLNDIQGRHVRK
                     EHVYDALESAKTGLVEEGAVGAGTGSSCMGWKGGIGTASRVVPNSLGGYTIGVLVQSN
                     FGGVLTVNGAPVGRELGHYTYQEHFDNGSREQLDDGSVMVVVASDAPLDQRQLRRLAA
                     RASLGLARTGFYGSNGSGDFFIAFSTAFRVPHSEPATLSAEVVAGDAISALFFAVVEA
                     TEEAVLNSMFKARTVVGRDGNVVEAIDIGDLLSVMRRYNSLDWDKSLPPYIS"
     gene            5174..5434
                     /locus_tag="PLM83_03480"
     CDS             5174..5434
                     /locus_tag="PLM83_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK70502.1"
                     /translation="MTSAESPEVPVLVRQEWCKGCGICIAMCPKKVLEAAADGKVVLA
                     RPEDCIKCETCEIMCPDFAIRVVGGRSKEVARGREDVADARA"
     gene            5421..6563
                     /locus_tag="PLM83_03485"
     CDS             5421..6563
                     /locus_tag="PLM83_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOK70503.1"
                     /translation="MPGHNGRDLRLLQGNECCAEAAILAGCRFFAGYPITPSTEIAEL
                     LSEKLPQVGGKFIQMEDEIASMAAVIGASLTGAKSMTATSGPGFSLKQENIGFASFTE
                     VPCVIVNVQRTGPSTGLPTAPAQADLMQARWGTHGDHPIVALYPSTVPEVFDLTIKAF
                     NIAEQLRVPVILLMDEVVGHMREKVAIPHQSEIEIVSRPQPDVPPEEYLPYAAGPDGV
                     PPMAPFGTGYRWHVTGLYHDETGFPTTAAPKVDRLMKRLYSKIESAADEITFTSDEFL
                     DDADTVVVACGITARSARRSVRLAREKGIRAGMVQLKTIWPFPYDLVGDIAGRVDRII
                     VPEMNMGQLVCEVERAAKGRCEVVGYSRVDGEPIAPAEIVAKIEEV"
     gene            6564..7391
                     /locus_tag="PLM83_03490"
     CDS             6564..7391
                     /locus_tag="PLM83_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOK70504.1"
                     /translation="MVAVPIDDYLRTQFMPHIWCPGCGHGIILGAVLRAIGRLDLPKD
                     EVCIVSGIGCASRAPGYLDFNTVHTTHGRALAFATGIKIARPDLHVIVLTGDGDAAAI
                     GGNHLIHAARRNVGLTTVMFNNSIYGMTNGQYSPMTPRGRYASTAPYGNVERNFDMCE
                     LVRAAGGNYVARATTAHARQLSNYVENAIQTPGFAFVEAVTQCPTYFGRRNKMGTPVD
                     MIEWFRKNSINAAAAAKLDPADLDGKIIIGEFYRGDAPEYTEVYDEVIERAKEAAAR"
     gene            7388..7933
                     /locus_tag="PLM83_03495"
     CDS             7388..7933
                     /locus_tag="PLM83_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOK70505.1"
                     /translation="MRREIRLSGYGGQGLILAGIILAEAAMHDGHEVVQSQSYGPEAR
                     GGASRAEVIISDEPIDYPKVTNPDVLLLMSDQACKRFAGSACQGGTVIVDSGLITSIP
                     ETSGVVYKFPITDIAVEATGRSISANVAALGVLNTVAKLVSPESLKNAVSSRVPRGTA
                     EVNLAALRAGIAAAEAAGAER"
BASE COUNT         1924 a         1838 c         2434 g         1895 t
ORIGIN      
        1 ggtgcctgcc agggttgggc atcgagtgcc tgtacagccg agacaggttg tagagagcat
       61 ggatgtgaat caaggaagcc tggtcattgg ctattggact ggcgtcaaca tgtccgaccc
      121 cgactatcca gcgattttga tggccaatgg catttacgga gggtatgcgc actcgaaact
      181 ctttcagaat gtccgcgaag gtgaaagctt ggcctactcc gtctattctg ggattgatca
      241 gaataagggc gtcatgaatg tttgggcggg gatagcgcct gaagccatgg ataaggcggt
      301 cgagataatc gaggagcagt tagatgatgt gaggtctggc aggatctccg atgacgaaat
      361 ggacaagact cgcagggcaa ttacgagaga catacgcatg atgggggaca gtccgggcgc
      421 tcagattgat tacgccatcg tctccaggat aaacggcgtt ggggagaaac ctgacgagct
      481 attgcggcgc atagaagcag tctctatgga tgacatagtc cgtgttgcga gcaaaataga
      541 gcttgacaca atctacttcc tgcgcaatga ggaggacgag gctggtgcgt agtatggaaa
      601 ttgcaaggtc agaactgctc cgggaggaag tatccactac ccgtctcgac tgcggcctgg
      661 aagtgtgcgt ccagcggaag gcggggtacg caaagaagta cgcccttatt ggcacgagat
      721 atggatcgct cgacaccaag ttccgcagga tcgatacggg agaaactgtg gatgtccctg
      781 acggattagc ccattttctc gagcacaagc tttttgcgga ggagtcagga tcgattgatg
      841 acgtctttgc ggagctgggt gcctactcaa acgctttcac agactataca atgactgcct
      901 acttattttc ctgtgtcgac aatttcgggc cttgtctcga taccctcctg gattttgtga
      961 tgaggcctca tttcactgag gagaatgtgg agaatgagaa aggcataatt gagcaggaga
     1021 tcaagaggga tgaagactcc cccgcctggc aggcgatgta cggaatgctg aaaggcatgt
     1081 atcagtgttt ccctgtccgc ttggaggttg cggggacagt cgattcaata cgctctatcg
     1141 acgtggacat tctgacaagg tgttacaata cgttctatca tccgtccaac atgacattgc
     1201 ttgttgtcgg cgacgttgat ccgaacgagg tggctgaccg cgcctcggcg catctggatg
     1261 ataagcttca cgggccagtg ccagcagtag aacgtctgta tccgcatgaa ccgcgagaag
     1321 ttgggatgaa acgtgtggag cgcagaatgc cggtatcgga gccattggtc tgttttggat
     1381 tcaaggataa cgatgtgggg tatgaaggcg ctcaacttct gcgcaaaact gtgtgcatgg
     1441 aaatgttgct ttacactttg atagggcgca gctccgcact gtacaacgaa ctttacgaag
     1501 cagggttgct cgaccacacg ttcgactgga atttcgattg cgaaaaggac tacagttttg
     1561 tgatattcga ggggaagagc aattctccgg atgctctggt ggaccgcgtt tgtaagggga
     1621 taaatgactc cattgcggca ggcatcgaca gagagctgtt tgagcgcgct cgccggagtt
     1681 tcataggctc tgcgctcaga gcattcaact ctttggagtt catagcctac aactttcttg
     1741 cttaccactt cagaaagatg agattgcttg acagagttgg ggttgctgag agcatttccc
     1801 tggacgaagt gatggatctc ggcaagcagc tgttcgatcc cgagatgctt gtagtatccg
     1861 tggtgaatcc catgcaatag agtttctggc aatgaggggg acaaacatga cgaaactgct
     1921 tttgatcaga cacggagaga ctatgttcaa tcgcgcaagg cgattgcagg gatgcaagga
     1981 tgttcccctc tcagatctcg ggacccggca ggcggacgcc tgtgctaact acatagcttc
     2041 cttaaatcgc ggcacgatta cagctctata ctcgagtcct cttgccaggg cgtacatgac
     2101 tgctagcgcc attggcaagg cggtcggctt agatgtggtg accgacgatc gactcaaaga
     2161 aatcgatgtt ggcgccctcg aaggaatgac ctgggacgag gtgcagctgg cttacccgga
     2221 tttcatggtc gaacatagaa ctgatcccat tgggaccagg tatcctggag gggaaagcgt
     2281 cgcggatgtc tcggcgcgag cacgcgatct tgccgacgga tttgtctgcg atcatggcga
     2341 tgagacaatc gtcgtcgttg gacatgctat tgttctgaaa gctctgattt gccgcgtgct
     2401 caatctggat gttgcgaatc acttgcggct cacagtgggc aatgcgtctt tgagcattgc
     2461 aagtgtttct cgggtggatg acgacgtaaa gggtaaggtt ttactgctca acgataggca
     2521 cttcctcgaa gcattaggac ttgacgcgcc gcggaaagag gggcatgagg aatgaaggct
     2581 gtgttatgaa aactgtgtgt cccttggtcg atacctgtgg acttatggca atggtgtcgg
     2641 agggtcgtgt agtcagaata aaaggagatc ccggccactc aattatgcgt gagttcatag
     2701 acaagaactc cgccgtgaca aaatatccac atgcttccgc ttatccggga ggcgtgatga
     2761 ttgggccaag gttgtgctca tttacggaat cgtcgtgcgg atccgttgcg acggagatat
     2821 gctgcgcttc gctatgcatt agggcgaggt gtttttttcg gctaggctcg acagtcgatc
     2881 cagacaggat gtggtcgtgg cttacaacgg tgagcctgtc aggtgtgggg gaggggtcag
     2941 ctttttcata ggagaacacg tgggcgatat cgggcttcag gccgcatttt acgattgcgc
     3001 cttcagtgtt gaggaagcac aagaatcagc agagaagtga aagtgcaaaa ctgagaatcc
     3061 acgggcaggt gatgttttca atgtgtggcg tttcagaatc aagtaggcct agggccaggg
     3121 agatagggat acacacgggc atcttttcgc ccggagagtt taatgccatt acagatgtgg
     3181 atggggtcat ggttggccat cgcactgtag tgcacggcga gggcgagctt gttcccggga
     3241 agggtcctgc cagaacagga gtgacggcaa ttcgtcccca tgggggcaat gttttccgtg
     3301 agaaggcgcc tgcggcagcc tatgttttca atggctatgg caagtccgta ggcattcacc
     3361 aaattaacga gacaggcgtg ctggaaacgc ctatattgct cacgaatact ctgaacgtag
     3421 gtcttgtcgc cgatgcattg attgaatggt ctgttgagcg aaatggtgat atcggcatct
     3481 caacgagtac tgtcaacccc gtggttgggg aggtgaatga cggctacttg aacgatattc
     3541 agggaaggca tgtccggaag gaacatgtgt acgatgccct tgaatctgcg aagactggac
     3601 tggtcgaaga gggagcagtg ggcgcaggca caggaagcag ttgcatgggg tggaaaggcg
     3661 gaattggcac ggcgtccaga gtcgtcccga acagcctggg cggatacacc atcggagtgt
     3721 tggtccagtc caacttcggc ggagtcctta cggtaaacgg agcgcctgtc gggcgggagc
     3781 tcggacatta tacctatcag gagcatttcg acaatggctc tcgggaacaa cttgatgatg
     3841 gctctgttat ggtggttgta gcttctgatg ccccattgga ccagcggcag ctgaggcgac
     3901 tggcggcaag ggccagcctc ggccttgccc gaacagggtt ctatggaagc aatgggtcag
     3961 gcgatttctt catagccttc tcaacagcct tccgtgttcc acattcagag cctgcaacgc
     4021 tcagtgcaga ggtcgtcgcg ggcgatgcga tatccgcact cttttttgca gtggttgagg
     4081 cgactgaaga ggctgttctc aactcgatgt tcaaggccag aacagtggta gggcgcgacg
     4141 gaaatgttgt tgaagccata gacataggtg atcttctcag tgtcatgcgc agatacaact
     4201 cgctagattg ggataagtca ctgccgccat atatctctta acgcatgcgt taagacggga
     4261 aacgtcatgg gaagttgaaa aagaggagct tacgggcgaa agcggcctct tcttctttat
     4321 gctcgctatg gtccaaagaa tcgcatgcaa ctgatgttgt ccttacaggc taggccttgc
     4381 ggagagcacc caagtactat gacaaatcgg cttatgttct ttagcattgt tactatggtg
     4441 ttggcagcca aggcaggttc atacaagatt gcatatgtcc gcatcaaagt gatagaattg
     4501 aagaggtgaa gtatacagtt gttctcattc ctcggttgcg tagtgaatgg ggccttttgc
     4561 atgaccttgc cattataaca tgtaggcagg caaaaacgag tttgggggta ggctggccta
     4621 tggccgtaac tgccgctgct gacgcgaggc gaagaaaaat gaaatggttg atgccctgga
     4681 ctggtttgaa agtaaaaatg attcccgtct atcccgtaca gaaggcaatc ggttttcctg
     4741 gcctaggtaa acacggggat gttggggcgt taatcgccag tgcacaaggg gttactgaga
     4801 tgatcgaatg cccggacgca tgtgcaaccg aattgaggat gacttgcagg attgtacgct
     4861 atacgataca gaggcgttct gtctgtggct gtgacctttc cttgtctgtt tggcccgcac
     4921 tcgtttgcgg gctgtcttta tttctgtgag gatttgttgt tggatcgaac tgcgtgaaca
     4981 tcgtgaacat atggaatgat gaacgctggc aggcgatgtc ctgaacatgt tctgcagcta
     5041 tatgccggat agggtagagc gccccttggc tgttggcaaa agctgtcgca gagggctgct
     5101 cggccgagat cccacatccg agttgcagac attcgttctc ggccaactaa ccaaaagatg
     5161 tggaggggag tccatgactt ctgcggaaag ccccgaggtt ccagtcctcg tgaggcagga
     5221 atggtgtaag ggctgtggta tatgcatcgc catgtgccca aagaaagttc ttgaggcagc
     5281 ggcagacgga aaagttgttt tagcaagacc tgaagattgt atcaagtgcg aaacctgtga
     5341 gattatgtgc cctgattttg ccatacgtgt ggtgggcggc agatccaagg aagttgcacg
     5401 gggaagggag gatgttgcgg atgcccgggc ataatggcag ggatcttagg ctattgcaag
     5461 gcaatgaatg ctgcgcggaa gctgcgattc tcgcaggatg caggttcttt gcaggctatc
     5521 cgataacgcc gtctacggag attgccgagc ttctctcgga aaagctccca caggtcggcg
     5581 ggaagttcat acagatggaa gacgaaatag ccagcatggc agcggttata ggggcttcac
     5641 tcactggtgc caagtcaatg acggctactt ctggtcccgg cttttctctg aaacaggaga
     5701 atatcgggtt tgcttcattc accgaggttc catgcgtaat agtgaatgtt cagcgcacgg
     5761 gaccgagcac gggcttgcct actgccccgg cgcaagcgga tctgatgcag gcgagatggg
     5821 ggacgcacgg tgaccatccc atagttgccc tctacccatc cactgttcct gaggtatttg
     5881 accttacgat caaggcgttc aacatagcgg agcagctcag ggtaccggtt attcttctca
     5941 tggacgaagt tgtggggcac atgagggaga aagtggcgat tcctcatcag tctgagatcg
     6001 agatagtcag caggcctcag cccgatgttc cgcctgagga atacttgcca tatgcggcag
     6061 gccctgacgg cgtgcctcct atggcgccat ttggaacggg atacaggtgg catgttacgg
     6121 gattgtatca tgacgagacc gggtttccaa caacggcagc gccgaaagtt gaccgactta
     6181 tgaagcggct ttactcaaag atagagtctg ccgcggatga aatcacattc acatcagacg
     6241 agttcctgga cgatgcagat actgttgttg ttgcctgtgg cataactgct agatccgctc
     6301 gccgaagtgt aaggcttgca cgtgaaaagg ggattagggc aggaatggtt caactgaaga
     6361 cgatttggcc gttcccatac gacctcgttg gcgatatcgc aggcagggtt gacagaatca
     6421 tagtgcctga gatgaacatg gggcaattag tctgtgaagt tgagcgtgca gccaagggca
     6481 ggtgcgaggt tgtgggttac tcccgagttg acggcgaacc catagcccca gctgaaatag
     6541 tagccaagat agaggaggtg tgactggtgg cagtgcctat tgatgattat ctacggactc
     6601 aattcatgcc gcatatctgg tgcccaggat gcggacacgg cattattctc ggcgctgttc
     6661 tgagagccat cggcaggttg gatcttccca aggacgaagt gtgcatagtg tcagggatag
     6721 gctgtgcttc tcgtgctccc gggtatctgg atttcaacac ggttcacaca acacacgggc
     6781 gggctctggc atttgccact ggaataaaga tcgccaggcc ggatctgcat gtgatagttc
     6841 tcacaggaga cggcgatgct gccgccatcg gcggcaacca cctgatccac gcggccagga
     6901 ggaacgtcgg cctaaccact gtcatgttca acaactcgat ctatggcatg accaatggtc
     6961 agtattcgcc tatgacccct aggggaagat acgctagcac tgctccatat ggcaacgtgg
     7021 aacgaaattt cgacatgtgc gagctcgtca gggccgctgg cggcaattat gttgctcggg
     7081 ccacgacagc ccacgccagg cagttgtcga actacgtgga gaacgctatt cagactccag
     7141 gatttgcgtt cgttgaggct gtcacccagt gtcccacata ctttggacga aggaacaaga
     7201 tggggacccc tgttgacatg atagagtggt tccggaaaaa ctccattaat gcagcagccg
     7261 ctgcaaagct ggacccagct gatctagacg gaaagataat aataggtgag ttttatcgag
     7321 gagacgcccc cgagtacacc gaggtgtacg acgaagtaat cgagcgggca aaggaggcgg
     7381 ccgccagatg agacgtgaga ttaggctctc aggctatggt ggacaggggc tgattctggc
     7441 cggaataatc ttggcagagg ctgcaatgca cgacggccat gaggtagttc agagtcagag
     7501 ctatggaccg gaggcacgcg gcggcgcttc cagagcagag gttatcatta gtgacgagcc
     7561 gatagactat ccgaaagtga caaaccccga tgtgcttctt ctcatgtcag accaggcatg
     7621 caaacgcttt gcgggaagcg cttgccaggg aggaacagta atagtcgact ctggacttat
     7681 cacttccatt ccggagactt ccggggtcgt ttacaagttc cccattaccg acattgctgt
     7741 ggaagctaca ggcagatcaa taagcgccaa cgtggcagct ctgggcgtac tgaatactgt
     7801 ggccaagttg gtttctccgg aatcgttgaa aaacgcggtt tcgtctagag tgcctcgggg
     7861 cactgccgag gtcaacctgg ctgcattgag agcagggata gctgccgctg aggctgcagg
     7921 tgcagaaaga taggtggaga atactagatt tgggccgaca tgatccaaca tagttaggag
     7981 agtgatgatc cattggagaa ggttgcattc gcaactagga tgcacgatct cggcggtgat
     8041 gtcgttcgca acatgttttt gctcaccgag aacccggaca tcatctcttt t
//
LOCUS       DAOSAI010000062         8076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5793_length_8076_cov_3.922204, whole genome shotgun sequence.
ACCESSION   DAOSAI010000062 DAOSAI010000000
VERSION     DAOSAI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5793_length_8076_cov_3.922204"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1490
                     /locus_tag="PLM83_03500"
     CDS             171..1490
                     /locus_tag="PLM83_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOK70506.1"
                     /translation="MMEDGSLPASAQGTARPIRETERSLLGIVMLMSLTAGFSLSLAR
                     NGTLTGEEAALCFVSSAPFAMTHLVFVAKGFDGDELILPLAAAATVMGYPVVYRLRPD
                     LAVRQLAFIWLGSAVMAVLAILQNQKKLEGWGVPALGGSIALLVLTLMVGEERGGARS
                     WLFLGDMGFQTSELAKITCILAGAEGLSKMHTADSMATARASAREIWECSQARRVIVM
                     WAAVMLLTVAQRDVGTAMVIYISCVAMAYVATGNRLLVLVSIGIGAAAVAVAGRVFPH
                     VGVRFSTWLNPWADAAGSGYQVTQSIYALASGGILGRGYGCGSPWLIPAAVTDMLFSA
                     SAEEIGLAGSIGLIAIYFLMVGHGYACAQRADSRYSALVAVGAANMIAVQTFVIIGGN
                     LGVLPLTGVTLPLASYGGSSMLTSLAIVGILLGSTRRLHSIDGGALS"
     gene            1487..2893
                     /locus_tag="PLM83_03505"
     CDS             1487..2893
                     /locus_tag="PLM83_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOK70507.1"
                     /translation="MNRRIRRLVQAWIAGCAILVVAATYYQAISADKLSSSQANPRLA
                     NLERDVIRGPILDRNGGVICDSVGPGLSTRVWRGPDSLASLVGYVNPVLGKSGLEAAY
                     DVWLSGRRGPYDVTGLLATLAGQPDHGCAVRLTIDANVQKACESALRGRRGAAIVINA
                     GTGELLAVASSPWYSMKGLELSWDNIVSRNDSPLVCRPINGMYPPGSTIKMAIAAIAL
                     DSGVTDPNEVYECEGSIRAGGSVVHDPTGAHGAIDIVDALAVSCNTTFVDLGLRLGPK
                     LYEELRRFFFDRSVPLDIPTPPANLDVRNADVSGDAIGQLAIGQGNLLVTPMHVAMMA
                     AAVANRGIMMRPYVVADISAVDGVPVMVASERALASVMTPSSADIVARGMRQAVKRGT
                     ARSAARWGYDIAGKTGTAQNPHGNPHAWFAGFGVNGNNRVAAAIILENAGSGGTAAAP
                     LGGDIIRVALDSMDRARR"
     gene            2898..4895
                     /gene="pknB"
                     /locus_tag="PLM83_03510"
     CDS             2898..4895
                     /gene="pknB"
                     /locus_tag="PLM83_03510"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOK70508.1"
                     /translation="MTGEILGGRYSILDTIGEGGMAIVYRAKDMLLNRVVAIKVLRGQ
                     YASDSEFRERFRREAQSAAALSHPNIVNVYDVGEDSGSNYIVMEWVDGQTLNDIIMRD
                     GRLTVDVVADYSVQILDALDHAHRSGVIHRDIKPHNILITRDGRVKVTDFGIARAASA
                     SALTEAGQVIGTVNYTSPEQARGASAIAESDIYSLGVVMYEMLTGRLPFVGDTPVAVA
                     LKHVQEDALPLSVYNKAVPVEFERVVMKALAKDPQDRWHTARAFKNAILRAHPTEVKK
                     VPDGQQVEDLPTASGSEGAATPHSRITNGVDDDLNITRPFDKVGEAFELENDQETPKR
                     ADRQAKTWTTIIVVMLVVAGAVLGALYSFRQWIQVEEVVVPDVVNMTLPDAESLARRS
                     RLALDSSVKRYDNKVEYNRIIEQDPPAGAKRKVHSPIKVIVSLGPEMVTVPDLFNKSE
                     REARLELERSNLVLGAKSQDYHEDVRPGRIVDQEPEVGTYVPRGSTVNYTVSIGPPPP
                     PPEVPLVIGMTLERATDTLKRAGFAVGEVRKRISILYEDGTVIEQNPGPYQMAESGTT
                     IDLVVAQRSGFPSASPSPSDDGGGQIDMSYRRYDVTYQVQPGSEVQEIVIKVNDSYGE
                     RTSYGPKLHRPGDWIRHTVEVYGPGKIEVWVDGYPVKTENV"
     gene            4888..5775
                     /gene="rsgA"
                     /locus_tag="PLM83_03515"
     CDS             4888..5775
                     /gene="rsgA"
                     /locus_tag="PLM83_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOK70509.1"
                     /translation="MYDIGRIVRISGGLCWVLPDGEQETVPCAARGNVKRREEGVAVG
                     DRVRMLRQNGTGVVEEVLPRRTYLVRPTVANVDQAIVVAAAAQPDPNPCLLDRLLVMS
                     EYAGLDAVVCINKADLVTRDECESLLRLYSDIGYKTILASARTGLGLEALSAALAGKT
                     SVFAGPSGVGKSALLNMVQPGHDLVEGEISEKIGRGRHTTREVRLIPLDVGGFVADTP
                     GFSSLDITSIAPRELTHVFPEISRLASECKFVGCLHDKEPDCAVKEAVERGVVDPGRY
                     AHYLSFLEELREAEKRQYR"
     gene            5910..6599
                     /gene="rpe"
                     /locus_tag="PLM83_03520"
     CDS             5910..6599
                     /gene="rpe"
                     /locus_tag="PLM83_03520"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOK70510.1"
                     /translation="MIKIAPSILSADFGILAEEIRGIANDADMIHIDVMDGSFVPNIT
                     LGQPVVRRIRQATDLPFDVHLMIVNPEEHIDSFTDAGADAITVHAEASRHLHRTLEGI
                     RQRGVRAGVALNPATPVCAIEHVLHMVDMVLVMTVNPGFGGQSMIVEALNKVEAIAAM
                     ARRNGLSFDIEVDGGINDETIGAAARAGANVFVAGSYVFGAENPREAIGVLRAAAIAA
                     AESSQRTQQMI"
     gene            6753..7355
                     /locus_tag="PLM83_03525"
     CDS             6753..7355
                     /locus_tag="PLM83_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70511.1"
                     /translation="MKLPGINIKVVLASVAVAAVLLFGGREVFLRTSVAMPMDRAFAS
                     MSEIESYSFERSAYGAVVEVKLGNVEDLSETVRKVEAAVAKLPADGRLWVRISDTRDV
                     LLQDVYYRMHFDIEEAIATGSFSDLKGRADEKARQAGLSGCVVQVDADNVYVSLENGE
                     HRLYAVVSRASGAVQGGTVVRSDARWLSTCSPRGKAGVGR"
     gene            7352..>8076
                     /locus_tag="PLM83_03530"
     CDS             7352..>8076
                     /locus_tag="PLM83_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK70512.1"
                     /translation="MIRDVLIGTGIAAFLFCIVYGYDVTPIIFAGGLYAAFELMMSGR
                     VGDRKFEVIQEAESGGRVSKLTFGDVGGQETAKRELVEALEFINNEEKAVKLGIRPLR
                     GILLSGPPGTGKTLLAKAAANYTDAVFISVAGSDFIEMYAGVGAKRVRDLFGTARSTA
                     RACKKKTAVIFIDEIDVLGGVRGTHQGHLEYDQTLNQLLVEMDGLTSASREGVRVLVI
                     GATNRADLLDPALLRPGRFDRLV"
BASE COUNT         1716 a         1901 c         2590 g         1869 t
ORIGIN      
        1 gagacgatca agaggttcca tgaccctagg gaggcatgca tggagcttgt cagattagcc
       61 cgtgcgcgcg gcgggcatga caacataact gtagtccttg tccgagtcga ggaatcggat
      121 gtctcatcgt cttcgtcttc tgcccgcgcg tgttcagggg gactagtgat atgatggagg
      181 acggcagtct gccagcgagt gcccaaggca ctgcgcgtcc cataagggag acggaacgtt
      241 cgctattggg gatagtcatg ctcatgtcgc tgacggctgg attctctttg tcgcttgcta
      301 ggaatgggac gctgaccggc gaggaagcag cgctctgttt cgtgtcatca gcgccatttg
      361 ccatgactca tctggttttt gttgctaagg gtttcgatgg cgatgagtta atactgccgc
      421 ttgcagctgc ggcgacagta atgggttatc ctgtggtata caggctcagg cctgacttgg
      481 cagttcggca gcttgccttc atatggcttg gatcggctgt tatggctgtt cttgctattc
      541 ttcagaatca aaaaaagctg gagggctggg gagttccggc tttgggcggg tcaattgctc
      601 tgctggtcct cactttgatg gtaggagagg aacgcggagg agctcgctca tggctgttct
      661 tgggcgatat ggggtttcaa acgtctgagc ttgccaagat cacatgtata ttggcgggtg
      721 cggaagggtt gtcgaagatg cacactgccg attccatggc gactgccaga gcgtccgcaa
      781 gggagatatg ggagtgttct caggctagaa gggtgatagt gatgtgggcg gctgtgatgt
      841 tgctcactgt ggcgcagcgc gatgtgggca ctgccatggt catatacatt tcctgtgttg
      901 caatggccta cgtggccacg ggaaacagat tgttggttct agtttcgatt gggattgggg
      961 cagctgctgt ggcggtggca gggagggtct tcccacatgt tggcgtcaga ttttccacgt
     1021 ggctgaaccc gtgggctgat gcagcaggtt caggttatca ggtaacgcag tcgatctatg
     1081 ccctggccag tggcgggata cttgggcggg gatacggatg cgggtctcct tggttgatcc
     1141 cggctgcagt taccgacatg ttgttctcgg catctgctga ggagataggg cttgctggat
     1201 cgatcggctt gatagcgata tactttttaa tggtagggca cggttatgca tgcgctcagc
     1261 gggcggattc aagatactct gcactggttg cggtgggtgc tgccaacatg atagccgtgc
     1321 agacatttgt cattatcggg gggaatctgg gtgtattgcc cctcacaggc gttacgcttc
     1381 ccttggcgag ttacgggggg agctccatgc tgactagcct ggccatcgtc ggaattctgc
     1441 tcggctctac gcggcgcctg cattccattg atggaggcgc tttgagttga acaggaggat
     1501 acgcaggctt gtccaggcgt ggattgcggg atgtgccatt ttggtggttg cggcaacata
     1561 ttatcaggca ataagcgccg ataaactctc ctcaagtcag gctaatccgc ggttggccaa
     1621 ccttgagcgg gatgtgattc gcggaccgat tctggaccgt aacggtggtg tgatatgtga
     1681 ttcggtcggc cctgggctct cgacgagagt atggcggggg ccggattcgc ttgcgtcgct
     1741 tgtaggctat gtcaatcccg tgctgggcaa gtcggggctg gaggccgcat atgatgtttg
     1801 gctttccggc aggcgtgggc catacgatgt gacaggtctg cttgcgaccc ttgccggcca
     1861 gcccgatcat ggttgtgcgg ttcggttgac gatcgatgcc aatgtgcaga aggcgtgcga
     1921 gtcggcattg agagggcgca gaggagctgc gattgtgatc aatgcgggga ctggagagct
     1981 cctcgctgtt gcgagcagcc cgtggtattc tatgaaaggg ttggagctgt cgtgggacaa
     2041 catcgtttcc cggaatgatt cgcctcttgt ttgtcgccca atcaacggga tgtacccgcc
     2101 tggatccaca atcaagatgg ccattgcagc catagccctc gacagcgggg taaccgaccc
     2161 taatgaggtc tatgagtgcg agggttctat cagggcgggg gggtctgttg ttcacgatcc
     2221 aacgggcgct catggggcga tcgatatagt tgatgccttg gctgtctcct gcaacaccac
     2281 ttttgttgat ctcgggctta ggctgggtcc gaaactatat gaggagcttc gacgtttctt
     2341 ctttgatagg agcgtacctt tggatatccc gacaccgcct gcgaaccttg atgtgcgcaa
     2401 tgctgatgtc agcggagacg ccattgggca gttggccata gggcagggga atcttcttgt
     2461 gactcccatg cacgtggcaa tgatggcagc tgccgttgcc aataggggaa taatgatgcg
     2521 cccctatgtt gtggccgata tttctgcagt tgacggcgtc ccggtcatgg ttgctagtga
     2581 aagggctctt gcatccgtca tgacgccatc atccgctgat atagttgcca gaggcatgag
     2641 gcaagcggtg aaacgaggga cggcccgttc agcggcgagg tggggatatg atattgccgg
     2701 caagaccgga acagcgcaga atccgcatgg aaatcctcat gcatggtttg ctggttttgg
     2761 cgtgaacggc aataacagag ttgctgctgc gataatcttg gaaaacgccg gctcaggcgg
     2821 gacggcagcg gcgccgctgg gcggcgatat tatcagagta gccctcgatt ctatggacag
     2881 agctaggagg tgagcggatg accggtgaga ttctgggcgg acgatattcc atactcgaca
     2941 ccatcggcga aggtggcatg gccatagtgt atcgggcgaa ggatatgttg ctcaacagag
     3001 tagttgccat caaggtgttg cggggacagt acgcctcgga ttctgagttc agggagaggt
     3061 ttagacgaga ggcccagagc gcggcggctc tgtctcaccc taacatagtc aacgtatacg
     3121 acgtagggga ggattccggt tccaactaca tcgtcatgga gtgggtggac ggccagacgc
     3181 tcaacgatat cattatgcgt gatggtcggc ttaccgtgga tgtagtcgca gattacagcg
     3241 ttcagattct tgatgcattg gatcatgcgc accgcagcgg cgttatccat cgcgacatca
     3301 agcctcataa cattctaata acgagggatg gtcgcgttaa ggtgacggac ttcgggatag
     3361 cgcgcgccgc atcggcaagt gctcttaccg aggcagggca ggtgatcggc actgtcaact
     3421 atacttcgcc ggagcaggcc cgtggagcct ccgccattgc cgagtcagac atatactcgc
     3481 ttggcgtagt gatgtatgag atgcttacgg gtaggcttcc atttgttggg gatactccag
     3541 tggcagtggc gctcaagcat gtgcaggaag acgcattgcc cctttcggtg tacaataagg
     3601 cggtgcctgt ggagttcgag cgagtggtga tgaaggcgtt ggccaaggat ccgcaagaca
     3661 gatggcatac ggccagggca ttcaagaatg ccatattgcg tgcccatccc acagaggtca
     3721 aaaaggtgcc cgatggccaa caagttgagg atcttccgac ggcttccggc agtgaagggg
     3781 cagctacacc ccattcgcgc attaccaatg gcgtagatga tgacctcaac attaccaggc
     3841 cgtttgacaa ggttggggag gctttcgagt tggaaaacga tcaggagacg cctaaacggg
     3901 cagatcgcca ggcaaagaca tggaccacga ttatcgttgt catgttggtc gtggccggtg
     3961 ccgttctggg cgcgctctat tccttccgtc aatggattca ggtggaagaa gtcgtagttc
     4021 ctgacgttgt taacatgacg ttgccggatg ccgaaagcct ggcgcgtcgg agtaggcttg
     4081 ctttggattc atcggtaaag cgctacgaca acaaagtcga gtacaatcgc attatcgagc
     4141 aagatccgcc tgcaggcgcc aaaaggaagg tccattcgcc gatcaaggtg atagtaagtc
     4201 ttgggcctga aatggtgacg gttcccgatc tctttaacaa gtccgagagg gaagcccggc
     4261 ttgaactgga acgttcgaac ttagttcttg gcgccaaaag ccaagattat catgaggatg
     4321 tgaggcccgg gcgcatagtc gatcaggagc cggaggtcgg cacatacgtg ccgaggggct
     4381 cgacggtgaa ctatactgta tccataggcc cgccaccgcc tccgcctgag gttccgctcg
     4441 taataggaat gactctggag cgggccacgg atactctgaa gcgggcgggc tttgcagtgg
     4501 gcgaggtaag gaagaggata agcatcctct acgaggatgg cacagtcata gagcagaacc
     4561 ctgggccgta ccagatggcg gaaagcggaa ccacgatcga cctcgttgtg gctcagagat
     4621 ctgggtttcc gtctgcttcc ccttctccat cggacgatgg cggtggccag atagacatgt
     4681 cgtatcggcg ttatgatgtg acctatcagg ttcagccggg gtctgaggtc caggagatag
     4741 tgatcaaggt gaatgatagt tacggggagc gaacaagcta tggccccaaa ttgcacagac
     4801 ctggggattg gatacggcac actgtggaag tttacgggcc cggcaagatc gaagtgtggg
     4861 tagacggata ccctgtgaag acggaaaatg tatgatatcg gtcgcatcgt aaggatttct
     4921 ggcgggctgt gttgggtgtt gcccgacggt gaacaggaga cagttccgtg cgccgcgcgg
     4981 ggcaatgtga agcggcgcga agagggtgtg gcggtcggag atcgcgttag gatgctgcgg
     5041 caaaacggca ctggcgtggt tgaggaagtg ttgccccgac gcacatacct tgttaggccg
     5101 accgtggcca acgtggacca ggcaatagtc gttgcggcag ctgcacagcc cgaccctaac
     5161 ccgtgtcttc tggacaggct attggtcatg tcggagtatg cgggccttga tgcggtggtt
     5221 tgcataaaca aagcggatct tgttacgcga gacgagtgcg agtcgttgct tcgtctatac
     5281 tcggacatcg gctacaaaac catcctcgcc agcgcccgga ccggccttgg gcttgaggct
     5341 ctctctgctg cacttgccgg aaaaacatcg gtctttgccg gaccgtcggg ggtgggcaaa
     5401 tccgcgcttc tcaacatggt tcagcccggg cacgaccttg tcgaaggaga gataagcgag
     5461 aagatcgggc gaggacggca taccacaagg gaagtgcggc tcattccgct cgatgtggga
     5521 gggtttgtag ccgacactcc tggtttctcc tctttggaca ttacctcaat tgcgcctcgc
     5581 gagcttaccc acgtgtttcc cgagatttcc cgtcttgcat cggaatgcaa gttcgttgga
     5641 tgcctccacg acaaggagcc ggattgcgcg gtgaaggaag ctgtagagcg cggcgtcgta
     5701 gatccgggtc ggtacgcaca ttacctcagt ttcctggaag agcttcgcga ggcggagaaa
     5761 cggcagtata gataattgat gcacccttga tgtatggggg cttctggctc tttgagccgg
     5821 cggcgcgcca gtgttgcgta tcgcgttgca ggggggatgg acgcgaggcg taaagcctga
     5881 ggcatggacg atgaaagcag gtgttggatt tgataaagat agctccatct attctttccg
     5941 ctgattttgg aatcctggcc gaggagatca gagggatagc caatgacgcc gatatgatcc
     6001 acattgacgt aatggacggt agttttgtgc caaacatcac acttggacag ccggtcgtaa
     6061 ggcgtatccg acaggctaca gatcttcctt tcgacgtgca cctcatgatc gtcaatcccg
     6121 aggaacacat agattcgttt acagatgccg gcgcagatgc gataactgtt catgcagagg
     6181 cttctcgcca cctccatcgc actttggaag ggataaggca gagaggcgtc agggcgggtg
     6241 tggccctcaa tcccgctacg cctgtctgcg ccatagagca tgtcttgcac atggtggaca
     6301 tggtgctcgt catgactgtg aacccaggtt ttgggggcca gagcatgata gttgaggctc
     6361 taaacaaggt ggaggccata gcggcaatgg ctcgcaggaa tggcttgagc ttcgatatcg
     6421 aggttgatgg gggaataaat gacgagacta taggtgctgc tgcccgggct ggggcgaacg
     6481 tctttgttgc gggcagctat gtatttggcg cggagaatcc tcgggaggcc atcggtgtcc
     6541 tccgggcggc agccattgcg gctgcggaaa gctcgcaaag aacccagcag atgatctgat
     6601 tgcttgaaga gcggctaatt tcgctgtacc tgcttgcgcc cctctgtaga tgtacggagg
     6661 ggtttccacg tacttacaga gatattgcaa agagtgtatg ttataatcat ccgtgcagct
     6721 aaaaggccat atgccagaaa ggaggcgggg ccttgaagtt accgggaata aatatcaaag
     6781 tagtgctggc aagcgtggcg gtggccgcgg ttcttctttt tggagggcga gaagtgtttc
     6841 tcaggacctc tgttgcaatg ccaatggata gggctttcgc ttctatgtcc gagatcgaat
     6901 cgtattcatt tgagcgttcg gcttatggcg cagtagtgga agtgaagctt ggcaacgtgg
     6961 aagacctttc agagactgtg aggaaagtag aagcggccgt cgcgaagctg cctgccgacg
     7021 gtagactgtg ggtgaggata tcggacacgc gtgacgtgct tttgcaggat gtgtactaca
     7081 gaatgcactt cgatatagag gaagcgatag ccactggatc tttctcggac ctaaaggggc
     7141 gtgctgacga gaaagctcgg caggctgggc tatcaggttg tgttgttcaa gtggatgcag
     7201 acaatgttta tgtcagcctt gaaaacggcg agcaccggct ttacgcagtt gtatcgcgtg
     7261 cgtctggagc agtccagggc ggaacggttg tccgatccga cgcaagatgg ctctctacct
     7321 gttcgccccg gggaaaggca ggcgtgggca gatgataaga gatgtgttga taggcacagg
     7381 catcgctgcc ttcctgttct gtatcgtgta tgggtacgat gtgaccccta tcatctttgc
     7441 gggcggtcta tacgctgcat ttgagttgat gatgtcaggg cgggttggcg atcgcaagtt
     7501 cgaggtcatt caagaggcag aatctggcgg cagggtatcc aagcttactt ttggagatgt
     7561 tggaggtcag gagactgcca agcgagagct agtcgaagcc ttagagttca taaacaacga
     7621 agaaaaggcg gtcaagcttg gcatcagacc cctcagaggc attttgctaa gcggtcctcc
     7681 tggaacaggc aagacgctcc tggcgaaggc cgcggccaac tacacagacg cagtgttcat
     7741 ttcagtggcc gggtctgact tcatagagat gtacgcagga gttggagcca agcgcgtccg
     7801 ggatctgttt ggaaccgcgc gctccactgc gcgtgcgtgc aagaagaaga cagcagtgat
     7861 attcatagat gaaatagatg tcttaggcgg agtacgcggc acgcatcagg ggcaccttga
     7921 gtatgatcaa accctcaacc agctccttgt agagatggac gggctgacat ctgcttcccg
     7981 cgagggtgta cgggtcctgg tgatcggggc gacaaaccgg gcggacctcc tcgatcccgc
     8041 gcttcttaga cctgggagat tcgaccgcct ggtaga
//
LOCUS       DAOSAI010000063         8056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5814_length_8056_cov_4.007249, whole genome shotgun sequence.
ACCESSION   DAOSAI010000063 DAOSAI010000000
VERSION     DAOSAI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5814_length_8056_cov_4.007249"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1077)
                     /locus_tag="PLM83_03535"
     CDS             complement(214..1077)
                     /locus_tag="PLM83_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOK70513.1"
                     /translation="MQIVTDYAADISPEQLEGLEVHYLPLMITVDGKTYRSGVDITPD
                     QFYELQATAVEIPTTSQPSPGEFADLYRSLAQTDPDILSIHISSGLSGTVNVARIGAK
                     MVPEADVEVYDTKTLSAAQGWHVEMAAKAVRAGWEKSRIVAMLKQVTEASETLFTLST
                     LKYLIHGGRISHLKGLVASLLNIKPIIGVEKAGGTYVTRGQARTMTRAIDALADIVSS
                     MYPPETPMRAQILHSQNPTGVSRLQDKLDKVFVCNWLPTGPIAPVLGAHTGPGLVGIA
                     IAPEALFPQMP"
     gene            1537..2667
                     /locus_tag="PLM83_03540"
     CDS             1537..2667
                     /locus_tag="PLM83_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK70514.1"
                     /translation="MKGSVVMEIGGDFCFADIRCANGEPSYQNVLDGLSKGRPVSMLA
                     SGRTAYLNAWLNLKKGGSLVRRALLPSYLCYSMLSPFHRMGVETEHYRVFSDLSVDTD
                     DLMEKAFDDIQGTLVVALNYFGFPDSVDLLSALAEIRRRGGAVVYDATHSALCERPWP
                     HTTFEPNLCIMSLRKSLPVVDGAVCVWLDGSEPAFCSNEAQLSGFHSARALAMMMKSA
                     YVADGFGNRRDYVLLFAQAEAALDSSFSASAAMSPVSRSILKRVGIEEIRRVRRRNFR
                     RLLEELATCGGVHALISELPEDAVPLGFPVVVEDRDLLWRHLEHYGIHADIHWELPPE
                     VPEEEYPESAWLARRILTLPCDQRYSEEDMARMARVLKEYRP"
     gene            complement(2740..4077)
                     /locus_tag="PLM83_03545"
     CDS             complement(2740..4077)
                     /locus_tag="PLM83_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOK70515.1"
                     /translation="MSKFLHYTVPSVMGCLVTSLYLVVDGVFIARGVGETAVAAVTLV
                     LPLTMAFVALSMIFSVGGGNLVSTSRGAGDQARAVNVFRESIYMLLSIGIIFSILGTL
                     FADEIAIMLGATGELVSPAAEYTKYYMLFTVPVLLAIALSTFIRHDGSPKLSMRSMLY
                     GAVTNIVLDYVFVFPLRMGLRGAAIASGLGQIVTVITCMKHFIERRGILRIGKASFEW
                     EDIKDIFVLGFPSFLTEVSYSVMMYLHNLAVISQVGEIGVTAYGIVNYINNISYMALF
                     GIGQGIQPMVSYYYGRGKFDKGRQQYIYGIKASLVMSVLFLVICLFVGRPLIMIFASS
                     EDVIDLGYTILTYLNLAYVLLGVNLTTQMYLQSMHMPRYANIICVLRGFVFVKIGLAL
                     LPKVFGDAGIWGTLLFAEGMSLITALVIVSASAEPEQYPSPIGNSQTDHASRP"
     gene            complement(4149..4976)
                     /locus_tag="PLM83_03550"
     CDS             complement(4149..4976)
                     /locus_tag="PLM83_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK70516.1"
                     /translation="MIPIRAYYSIGEISRLFGMSIQTLRHYDRIGLLRPAYVNHDNNY
                     RYYSHEQFLQIKLIKYLKTLGFSLAQIRDIVNQDLSSEVIIELLREQEEAVNNRIDQL
                     VALRQDMRAFEERLAGLAERTFGEAFLDSKPERKVIAYDYINKPNANLAVGMRKILAD
                     IEREHSVLKYEPGMLVSYTEFAASGRVEFKKIIVELDDELDYPHHNILRLPAGPHATI
                     IYEGATEEGQKYYGKIVEFISDHGLSPKGDFIHAIAVSYVNSNGTGVSLHEVSVLTE"
     gene            5293..5940
                     /locus_tag="PLM83_03555"
     CDS             5293..5940
                     /locus_tag="PLM83_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70517.1"
                     /translation="MDTKRAFSAIAVASVLLLGVVGCFRQKASSNELRSYFPEKTGFT
                     WVYSGFAEYGHRMILDSITEVRSKGDVVHQISGMVEDMSGGESEQDFGFQLQYVFGGD
                     EVVERILHKGVVFPHRIDGLVMLKAPLEKGQKWSFESVNGNRVDAEITETGVDAEDRL
                     DYCKVEYTAPDPEMPDGLYHETRVFKKGIGVVSFTSTIMPDIEFSYTLTSVSSPD"
     gene            complement(6096..6617)
                     /locus_tag="PLM83_03560"
     CDS             complement(6096..6617)
                     /locus_tag="PLM83_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOK70518.1"
                     /translation="MFSENMLAELNKQYNYELYSGYLYMAMEAYFHGKGLSGFANFFK
                     VQTEEERAHARIFYEFIIRKGGTITLDSIEKPEADFDSPLDLFKKALAHEEFVTSRIN
                     KLVDIALEDKDHATNSFLQWFVDEQVEEEENFQGIVQKLELIGNDVHALLMLDAELGQ
                     RVFTVPAPLAQAQ"
     gene            6998..7432
                     /locus_tag="PLM83_03565"
     CDS             6998..7432
                     /locus_tag="PLM83_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70519.1"
                     /translation="MVLNTSLAIGPVMWDNFNCDSASWRENTLRFTRTLSDHGLGQLV
                     YDLLMEAFKADQAGMYLHSHSFLATLEDLLVQEGELESFIYTMGGKARPADPGDDAPL
                     PIPGPSPGPKMTGGDGPCDNDELCEQCVAGFCVYSICQSCSG"
     gene            7477..8034
                     /gene="grpE"
                     /locus_tag="PLM83_03570"
     CDS             7477..8034
                     /gene="grpE"
                     /locus_tag="PLM83_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOK70520.1"
                     /translation="MDAGQGRMDDDTIGLADVQTPCIHEKLDVLIAMFEEKIEYDEAK
                     DKAFNKLYEELDMFKNDFVDRRMKEVYADLLLLYDNIKSYISRDDVQAPENEVARECL
                     EYVLEEVLEVLYRRGIERMACESDAFDPQCQRAVRVERANSPGEDGKVVKVMKDGFIK
                     DDRVLRHQMVAVARWDGDGHERVDD"
BASE COUNT         1836 a         1939 c         2279 g         2002 t
ORIGIN      
        1 ctgcctaaac tgatgttggc tgtgatcgat attccctggc atagaacggt tatcggatct
       61 gctccgaggc ccgcttttac gaacatcgct atccccaagc ctatcaagga caggccggaa
      121 atcatcatga gaattcgaag cgcaagatcg cggagtttca tatgctcgct cctaacatgg
      181 tcatgattgt tcgctgcaga cggtgatagc ctcctatggc atctgcggaa atagtgcttc
      241 gggagcaatg gctatcccca caaggcccgg gcctgtatgc gcaccgagca ccggagctat
      301 cgggcctgtt gggagccagt tacaaacgaa gaccttatcg agcttgtcct gaagccgaga
      361 tacgcctgtc gggttttgcg aatggagaat ctgcgcacgc atgggcgtct caggaggata
      421 catcgaactg acgatatctg ctaacgcgtc aattgcccta gtcatcgtgc gtgcctgtcc
      481 acgtgtgacg tatgtgccgc ctgccttttc tacgccgatt attggtttta tgttgagcaa
      541 cgatgctacc agtcctttga gatggctgat tcgtccgccg tggattagat acttcaatgt
      601 tgagagggtg aagagggtct ctgaggcttc agtgacctgc ttgagcatcg ccactatccg
      661 ggatttttcc catccggcac gcacagcctt tgcagccatc tcaacgtgcc acccttgggc
      721 ggctgaaaga gtctttgtgt catatacttc cacatcggct tcaggcacca tttttgcccc
      781 gattcttgcg acattcactg taccgctcag cccggaggat atatgaatgg acagaatatc
      841 cggatctgtc tgcgcaaggc tgcgatagag atcggcgaat tctccgggcg aaggttgaga
      901 cgtggtaggt atctccactg ccgttgcctg taactcgtag aattggtcag gagtgatgtc
      961 aaccccgctg cggtacgttt tgccatctac cgtgatcatc aagggaaggt aatgtacctc
     1021 caaaccttca agctgttcgg gggagatgtc ggcggcgtag tctgttacaa tctgcatcaa
     1081 agcttcctcc tcggcgcacc tgtatttggt gagtgcgcta ttcgtgcaga cactctgcgg
     1141 tgcatgaatg catgcaactg acgcttgccg gcggatgttg tcgatgcctt tcggccccgc
     1201 attttcatgt ctatacctgt tctcaatgtt accattcaaa tgaccgcggc gccagcagat
     1261 ttgattgtgc gcgaataccg gcagctaggg accgggcatc agacaatgcg agtttggcga
     1321 cgactcaaat gcacataatc ggcatcggat tagactcgta gggatccata ttctcatctt
     1381 gaatgcaagc ctttgtcatt cgtcgcaaag actccgttcc aacccaattg ccccgatata
     1441 gtgctgtaaa ggggctttcg acctgttggg gtggaatagt aaggtcataa tgcgcgatgg
     1501 atggtgcgga gttatactta acatgcagtg cttgtggtga aagggagcgt cgtgatggag
     1561 atcggcggag atttctgttt tgcggatatt cgatgtgcga atggcgaacc gtcatatcaa
     1621 aacgtactgg acgggctgag caaagggcgt cctgtaagca tgcttgcttc agggcggact
     1681 gcctacctca atgcatggct caacctgaaa aaaggcggaa gtctggtgcg aagggcgctc
     1741 ttgccatctt acctttgcta ctccatgctc agcccgtttc acagaatggg agttgagacc
     1801 gagcattatc gggtcttctc agatctttct gtcgataccg acgatctcat ggaaaaagcg
     1861 tttgacgata tccaaggcac actcgtggtg gccctcaatt acttcgggtt tcctgactca
     1921 gtagacctgc tttccgctct cgctgagatc aggcgaaggg gcggtgcggt ggtttacgat
     1981 gcgacccaca gcgccctgtg tgaacgccca tggccgcaca ctacctttga gccgaatctg
     2041 tgcataatga gcctcaggaa gagccttccg gttgttgacg gtgccgtctg cgtctggctg
     2101 gacggaagtg agcctgcatt ctgtagcaac gaggcgcagc tgagcggatt tcactcggcg
     2161 agggcgttgg caatgatgat gaagagcgcc tacgtggctg acggattcgg gaatcgccgg
     2221 gactacgtgc tcctgtttgc tcaggctgag gcggcgctgg atagcagttt cagcgcgagc
     2281 gccgccatgt cgcctgtttc caggtcaata ctgaaaaggg tcggaatcga agagatcagg
     2341 cgggttcggc gcaggaattt tcggagactc cttgaggagc tggcaacatg tgggggcgtg
     2401 catgcactca tctctgaact cccggaagac gcggtcccgc ttgggtttcc cgtagtggtt
     2461 gaagatcggg acctgctctg gcgccatctg gaacattacg ggatacatgc agatatccac
     2521 tgggaattgc cgccagaggt ccctgaggag gagtacccgg agagtgcgtg gttggcacgc
     2581 cgcatcttga cgctcccttg cgaccagcga tattcggagg aagacatggc caggatggcg
     2641 agggtattga aggagtatcg tccatgatca ctccatactc ggcatacgtg gtgcaaagtt
     2701 cggcgctggt gcggggtttt aggcgaatcg cgccggtttt cagggtctgc tagcatgatc
     2761 tgtctggcta ttccctatcg gagatggata ttgctccggt tcggcgctgg cgctgactat
     2821 gacgagcgct gtaattaggg acattccttc ggcaaacaat agtgtgcccc agattcctgc
     2881 atccccgaac accttcggca gtaaggcaag gccaatcttg acgaatacga atcctcttag
     2941 cacacagatg atattggcgt atctgggcat gtgcatggat tgcagataca tctgcgtagt
     3001 cagattgacg cccagcagaa cgtaggccag gttcagatat gtcagtatcg tgtaacccag
     3061 gtcgatcacg tcttccgacg aggcgaatat cattataagt ggtcttccga cgaataagca
     3121 gataaccagg aacaagactg acatcacaag agacgctttt atcccgtata tatactgctg
     3181 gcgtcccttg tcgaacttgc ctcgtccgta gtagtagctg accattggtt gaatcccctg
     3241 gccgatgccg aacaaggcca tgtaggagat gttgttgatg tagttgacta taccgtaggc
     3301 ggtgactcct atctcgccga cttggctgat tactgccaaa ttgtgcaggt acatcattac
     3361 ggagtaagat acctcagtga gaaaggaagg gaacccgagg acgaatatat ctttgatatc
     3421 ttcccattcg aatgaggctt tgcctattct caggatcccg cgtcgctcga tgaaatgttt
     3481 catgcacgtg attacggtga caatttggcc cagccctgaa gctattgcgg cgccgcgcag
     3541 ccccatccgg agggggaaca cgaaaacgta gtcgagcacg atgttggtga ctgctccgta
     3601 cagcatagaa cgcatcgaca actttggaga tccgtcgtgt cgtatgaagg tgctcaatgc
     3661 gatggcgagc aggacgggca cggtgaaaag catgtagtat ttcgtgtatt cagcggccgg
     3721 gctaacgagt tcacccgttg cacccagcat tatcgctatt tcatccgcga acagggttcc
     3781 taggatgctg aagataattc ctatcgaaag cagcatgtat attgactctc tgaaaacatt
     3841 gaccgcccgg gcttggtcgc ctgcgcctct cgaagttgat acgagattgc ctccgcccac
     3901 agagaagatc atgctcaatg ccacaaaggc cattgtgaga ggcagcacta atgttacggc
     3961 agctactgct gtttctccaa ctcccctggc aatgaagacg ccgtctacga cgagatacaa
     4021 tgaagttact aaacatccca tcacggatgg gacggtataa tgcaagaatt tgctcacgac
     4081 tgaaaggcct ccctaacagg aaagcttaca gtctattgtt ggtatagagt caagctgacg
     4141 ggggaagatc attccgtgag aaccgatacc tcgtgcagag acactcctgt gccattgctg
     4201 ttaacatagg agacggctat agcgtgaatg aaatcgccct tcgggcttag gccatggtcg
     4261 gatatgaact ctactatttt gccgtagtat ttttggccct cttctgtggc gccctcatat
     4321 atgatagtgg cgtggggacc tgcgggaagt ctcaatatgt tgtggtgagg atagtcgagt
     4381 tcatcgtcta actcgactat gatcttcttg aactctactc ttcctgaggc tgcgaattcg
     4441 gtatatgata ctagcatacc cggctcatac ttgagtacac tgtgttctct ctcgatatcc
     4501 gcgagaatct tcctcatgcc aacggccaag tttgcattcg gtttgttgat gtaatcgtag
     4561 gctatgactt ttcgttcagg cttgctatct aagaaagcct ctccaaaggt ccgttctgcg
     4621 aggccggcca gcctttcttc gaaggcgcgc atgtcttgcc gcaaggcgac gagctggtct
     4681 attctgttat tgaccgcctc ttcttgctca cggaggagtt cgattatcac ctcggacgac
     4741 aggtcctggt tgacgatgtc ccggatctgg gccaatgaga agccgagggt tttcaggtac
     4801 tttatcagct tgatctgaag aaattgttca tgggagtagt acctgtaatt gttatcatgg
     4861 ttcacatagg ctgggcggag gagcccaatt ctgtcgtagt gccttagggt ttgtatcgac
     4921 atgccgaaaa gcctcgatat ttccccaata gaatagtagg ctctgatcgg tatcacctca
     4981 tgcccgcttt attggcttac ctcgtcagtt tatcgacgcg gaagcctgtt gtcaaaccat
     5041 ggccctagca ggcttgtagt gtctaagttg gcggcagaga ttgggcaagg tcgaagcgca
     5101 tatggattcg tgtggggcac cttatcagag ccattggatc cggttgagta gtggggactg
     5161 caagagcacc cgtgcagtgg gattacaaaa tcaaggggtc gcgccggaca atgaataaga
     5221 ggaaggcttc gcggcagcat agttgtggat tggctcaggc aattatccat tggatgaaag
     5281 gcgggtttgg ggatggacac caaaagggct ttctcagcca tcgctgtggc tagcgtgttg
     5341 cttttgggcg ttgtagggtg tttccggcag aaggcttcca gcaacgaact gaggagctat
     5401 tttccggaga aaacgggctt cacctgggta tactcaggat tcgccgaata tgggcacaga
     5461 atgatcttag atagcatcac ggaagtgcgc tccaaagggg acgtggtaca ccagatatca
     5521 ggcatggtgg aagacatgtc cggaggcgag tcagaacagg acttcggctt tcaacttcag
     5581 tatgttttcg gaggggatga ggttgtggag cgcatactcc acaaaggcgt tgtgtttccg
     5641 cataggatcg acggcctcgt gatgctcaaa gccccgctgg aaaaggggca gaagtggtct
     5701 tttgaatcag tgaatggaaa ccgtgtcgac gctgagatta ctgagaccgg ggtcgatgcc
     5761 gaggacaggt tggactactg caaggtggag tatactgctc ctgatcctga gatgcccgat
     5821 ggcctctatc acgagacgag ggtttttaag aagggtatcg gagttgtctc cttcacatcc
     5881 accatcatgc ctgatatcga attcagctac actctcacat cggtttcatc ccccgattag
     5941 cagactgcgc aaccgatatg cctagggcag atcaggagag aaagaggcac agcctttgcc
     6001 aacatgtcga ccacattggc gctttgggct gtgcctcaac tcattctagc tttgaatgcc
     6061 cgcaattgat ggacagctga taaaaccttg ggcccttact gtgcctgggc cagaggagcg
     6121 ggcactgtga agaccctctg cccaagttcg gcatcgagca tcagaagggc gtgcacgtcg
     6181 ttgccgatta gctcgagctt ttggactatg ccttggaagt tctcttcctc ttctacttgt
     6241 tcatccacaa accactggag aaagctgttg gttgcgtggt ctttgtcctc aagggcgata
     6301 tctaccagct tgttgattct ggaggtgaca aactcctcgt gcgctagggc tttcttgaac
     6361 agatcgagcg gactgtcgaa gtcagcctcg ggcttttcga tggaatcaag ggtaatggtc
     6421 ccacctttgc gaatgatgaa ctcgtagaag atgcgcgcgt gggcccgttc ttcttcagtt
     6481 tgtaccttga agaagttggc gaaacctgat aggcccttgc cgtggaagta ggcctccatg
     6541 gccatataaa gatagcctga gtatagttca tagttatact gcttgttaag ctctgcaagc
     6601 atgttttcgg aaaacatgat catgcctcct cgtatcctga gcacgtggtt gctcaggcaa
     6661 tttgtggact agcaattcta agtgtacact gccagcgcga gaaaattcaa gcggtgtttg
     6721 cttctgcagc catagcatga acgtaggtta gcgggtctgg cggaagggag tattgccaag
     6781 accttgcctg tcgaggatca agctgcatga gtgaagagcg cggggaccca tcctggttga
     6841 acaggcagct gatttatggg caactctgcc tgcgtccgtc atgttctgct ttgcctttgg
     6901 acgcaaatag tgaaggattc catagctggt tgtagaaacc ataaactagt tatgaaatct
     6961 gtgaagtgcc cttgggaggc ggacggagga tggagttatg gttctgaaca cgtcgttggc
     7021 gataggccct gtaatgtggg acaattttaa ttgcgattcc gcctcgtggc gtgaaaacac
     7081 gttacgtttc acccgcaccc tttccgacca tggactgggg cagctcgttt atgatctgct
     7141 catggaagcc ttcaaagccg atcaagcggg catgtatctg catagccact cgtttctcgc
     7201 taccctagaa gacctgcttg tccaggaagg agagctcgag tcctttatct ataccatggg
     7261 gggcaaggca cggccggctg atcccggaga tgatgcgccg cttcctatcc ctgggcctag
     7321 tcctggtccg aaaatgacag gaggcgatgg accctgcgat aatgatgaac tgtgtgagca
     7381 gtgcgttgcc ggattctgcg tgtattcgat ctgccaatcc tgttcgggat aggggcgcaa
     7441 aagcatgtgc aactcatggg gaaaggagaa ttctgagtgg acgctggcca aggcagaatg
     7501 gacgatgata cgattggtct tgcggatgtg cagacgcctt gcattcatga gaaactcgat
     7561 gttctcatcg ccatgttcga ggagaagatc gagtatgatg aggctaaaga caaggctttt
     7621 aacaagctct atgaggagct tgacatgttc aagaatgatt tcgtcgatag gcgcatgaag
     7681 gaggtctatg ccgacctgct tttgttatac gacaatatca agagctacat ctcgcgcgat
     7741 gatgtccaag cacccgaaaa cgaagttgcc cgggagtgcc ttgaatatgt cctcgaagag
     7801 gtcctcgaag tcctctatcg gcgcggcata gagcgcatgg cttgcgaatc ggacgccttt
     7861 gatcctcagt gccagcgagc tgtaagggtg gaacgcgcga acagccctgg agaagacggg
     7921 aaggttgtca aagtgatgaa ggacggcttc atcaaggatg acagggtttt gcgccatcag
     7981 atggttgcgg ttgcccgctg ggacggggat ggccacgaaa gggtggatga ctagatgggc
     8041 cgggcggtag ggattg
//
LOCUS       DAOSAI010000064         7988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5872_length_7988_cov_3.518215, whole genome shotgun sequence.
ACCESSION   DAOSAI010000064 DAOSAI010000000
VERSION     DAOSAI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5872_length_7988_cov_3.518215"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLM83_03575"
     CDS             <1..502
                     /locus_tag="PLM83_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOK70521.1"
                     /translation="DPEEKKKVLSEVARAVEAMSKEYIGALIVLEGETGLEDIAASGV
                     KLDSTVSAELLINVFTPNTPLHDGAVILRGNRILAAACILPLSDRHVTGRKLGTRHRA
                     ALGLSERTDAVAVVVSEETGAISLANDGEIVRGLRPEELRDRLESLFDHNTKDTVIRN
                     LRSNGR"
     gene            499..1407
                     /locus_tag="PLM83_03580"
     CDS             499..1407
                     /locus_tag="PLM83_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOK70522.1"
                     /translation="MKPVSPEAKDTSIKVLAVVLAIAIWAVAISDKQRTSPSPKIELV
                     VKAPVSLRNLPDDLIVTDLPETVVIRLRGPKDLESTALESTVAVVDLEGAKPGQNLFP
                     IEVVAPNGFELVRASRAVADLTLEKRAVVSLPVDIAIVAAPHFNVELEHDLTGALHLF
                     FAQKVPDDFRSEAESLAEGGASLGQGLSEGGTEPEKAADEEMGASAGSASGLHGGRLE
                     GSESELVDGLRSSVSVSVSPAEVSIEGPETAVSRVARAVAMVAVGHESTVVVPVLALD
                     IHGQTVSDVIVYPETVTVDFYSKAIW"
     gene            1457..2836
                     /gene="glmM"
                     /locus_tag="PLM83_03585"
     CDS             1457..2836
                     /gene="glmM"
                     /locus_tag="PLM83_03585"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOK70523.1"
                     /translation="MGKLFGTDGVREVANTGLTPELAFRLGRAGASSVLSRSGARPTI
                     IVGGDTRRSTGMLESALSAGINSAGVDVLNVGVIPTPAVAYLVVKFGADAGIVVSASH
                     NPAEYNGIKFFGPDGFKFPDEVEDIIEKRALAGGSGEMGSVLWSEDDGLPRPSGSGLG
                     MRKEAPDAWERYVEHAVESASCRLDGLRVVLDCANGASCLTTPAAFRELGAEIIVINA
                     SPDGDNINCACGSTHPDELAAVVVERGADMGFAHDGDADRVIAVDADGRIVDGDQIMA
                     LSAIHRHSRGGLPGRAIVATQYSNLGLTHAMESIGCSVLTADAGDRYVLQMMLEKNLV
                     IGGEQSGHVIFLEKATTGDGLITAIEVAGIAVETGKPLSELAAVMTKYPQIMVNVRVS
                     RKDDYYHSDTIAEAVKAAAAELGSSGRLLVRPSGTEPVVRVMGEGKDEAKVDRIVQSL
                     AEIIQSELC"
     gene            2868..3278
                     /gene="acpS"
                     /locus_tag="PLM83_03590"
     CDS             2868..3278
                     /gene="acpS"
                     /locus_tag="PLM83_03590"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOK70524.1"
                     /translation="MCCQRVLGIGVDIIEIRRFHRIAAVAPQGVAQRLYTAEELSDGR
                     IGRPEYLASRFAAKEAVMKSLGCGMDSIAFTDIEVFQDEYGQPHVRLTGSARARADSI
                     GARTVMISLSHSDEMVCAFAVSVGGGCNEGCDSR"
     gene            3247..4938
                     /locus_tag="PLM83_03595"
     CDS             3247..4938
                     /locus_tag="PLM83_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOK70525.1"
                     /translation="MVATRVATPDESRRIDRDAIELYKIDGIVLMENAGAAVARVAVE
                     IGAERSGWSAGRPSVCIFAGKGNNGGDGFVAARHLVGCGVDVSVYVTGSLDGISGDAR
                     TNLDILRNMGVDVVGLTCEEAWSAAADDLAVADVAIDAILGTGAKGAPCGEPARAISM
                     MSKSPVPIVAVDVPSGVDAGSGAVAGACVTAHTTVTFGLPKTGLLMYPGAAHVGYLLV
                     DRIGLPPALTDSPKIKTCIVCPDDVSAALPQRRPDAHKGDCGRVLVVAGSRGMVGAAA
                     LASMAALRAGAGLVYLAAPEGVAAQACAGLTEVIVRPLPETSSGSIAGIAVDRVLDEA
                     AKCDAVVVGPGLTCSDDSRLIVEALIAEGQTPMVIDADALNLLSGRVDTLQRASAPVV
                     LTPHPGEMSRLVEAGVEEINADRLEWARRVAHRSGAVVALKGARTVTATPDGIAYLNL
                     TGNSGMATAGSGDVLAGVIGSLMAQGVPPQWAAVSGVYLHGLAGDYAAREVGLHGMVA
                     GDILLRIPKAYHDIVEESAGLWDMMSVRSAQYKKEVVNPAARGLSARNEANVGRS"
     gene            4913..6031
                     /gene="alr"
                     /locus_tag="PLM83_03600"
     CDS             4913..6031
                     /gene="alr"
                     /locus_tag="PLM83_03600"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOK70526.1"
                     /translation="MRPTWVEVNTGNIARNLRQIKSTLPKDTQILAVVKADGYGHGAA
                     PVAIRALEEGVSFLGVALVEEGVALRRAGIAAPILFMEDLFPWQALDLVRYGLTATIA
                     SYESAEMLSEAAMALGKKAKAHVKVDTGMGRLGLPPNEIANLVRRVAELPGIEIEGIF
                     THLACAECNSEMTGRQLEQFDDITEKLRREGHEIPIRHVANSAAMLSLPEAAYDMVRV
                     GGIMYGLSPLGTVAPEKPWVPALSFHTRVSYVKLVGPGTPVSYGATYVTSGREIIATL
                     PVGYADGYPRALSNVGEVLIGGERCPVVGRVCMDQTMVSLPADSDVRVGDEVTLIGAQ
                     GGDRIFAEELAKAAGTIGYEIVCAISKRVPRVYVAGDE"
     gene            6270..6545
                     /locus_tag="PLM83_03605"
     CDS             6270..6545
                     /locus_tag="PLM83_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOK70527.1"
                     /translation="MTLLRKIVVSMPAGLLEELDVCARQSGRNRSEVVREAMRSYLSQ
                     HARSDLREKLKHGYIEMASINLELARECAAAEDEAFAVSERFLAGGD"
     gene            6548..6898
                     /locus_tag="PLM83_03610"
     CDS             6548..6898
                     /locus_tag="PLM83_03610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOK70528.1"
                     /translation="MDVKRGDVFYADLNPVVGSEQGGLRPVLVIQNDIGNYYSPTTIV
                     AALTSRIKRAKLPTHVELPAGQGYLAVDSVVLLEQLRTIDKTRLHDRICTLDEEIMKR
                     VNRAAEISLGLLEI"
     gene            6963..7688
                     /locus_tag="PLM83_03615"
     CDS             6963..7688
                     /locus_tag="PLM83_03615"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOK70529.1"
                     /translation="MPPLILISSDFESGTNGKIAVGVEYAKAIYRSGGMPLILAPPMG
                     CADSNRVAHGILSDACGLLLTGGDDVDPRFYGESHAHPKIGAINPVRDEFEIALAKAA
                     LKLDIPVLGICRGAQILNVAAGGGLIQDIASCVDGAHAHRVSAPRWHPTHRVMLARNS
                     ILFGIFGEETIWVNSFHHQAVGPVAPGFSVTGVADDGVREAIEKPGSRFVMGVQWHPE
                     GLAERDAQSLRIFTEFVNAVRGV"
     gene            7702..>7988
                     /locus_tag="PLM83_03620"
     CDS             7702..>7988
                     /locus_tag="PLM83_03620"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOK70530.1"
                     /translation="MSAPLVQVTRGELVESLHRGHIAVVDCRGNVLWQAGDPMYVTYF
                     RSSAKPFQVLPIIESGAVDRFGITERELAVMAASHHGEDCHVEAVMSILGK"
BASE COUNT         1714 a         1814 c         2634 g         1826 t
ORIGIN      
        1 cgatccagag gaaaagaaaa aggtgctgag cgaagtggct agggccgtgg aggccatgtc
       61 gaaggagtat atcggagccc tcattgtctt ggagggagag accggacttg aggatattgc
      121 agcatcagga gtgaagcttg atagcactgt atctgctgag ctgctgatca acgtctttac
      181 tcctaacact ccgcttcatg atggggctgt aatactgcgt ggaaaccgaa tactggcggc
      241 tgcatgcatc ttgccgcttt cagaccgcca tgtgactggg cgcaagttgg gcactaggca
      301 cagggctgcg cttggtctta gtgagcggac tgatgcggta gcagttgtag tgtcagagga
      361 gacaggcgca atatcgctgg ccaacgatgg ggagatagtt cggggtttga gacctgaaga
      421 gcttcgcgac aggttggaaa gtttgttcga tcacaacacc aaggatactg tgatacgtaa
      481 tctaaggagc aatggcagat gaaacctgtt tcaccggagg ctaaggatac gtctatcaaa
      541 gtgttggctg tggtgttggc catcgcaata tgggctgttg caatcagcga caagcagagg
      601 acttcgcctt caccaaagat tgaactggtg gtgaaagcgc ctgtttcgtt gcgaaatctc
      661 ccggacgacc tgatagtaac ggatttgcct gagactgttg ttataaggct cagggggccg
      721 aaagatctgg agtcgaccgc tttggaatct accgttgccg ttgttgacct tgaaggggct
      781 aagccgggcc agaacctatt tccaatagag gtggtggcgc ctaacgggtt cgagcttgtg
      841 agggcgtcgc gagcggtggc tgacttgact ttggagaagc gtgccgtcgt ctcgcttccg
      901 gtggacattg caatagttgc ggcgcctcat ttcaatgtcg agcttgagca cgatctcact
      961 ggcgcgttgc acctcttttt tgcgcagaag gtgcccgatg acttccgttc tgaagcagaa
     1021 tctcttgccg aaggcggggc gtcattggga cagggactgt ctgagggagg aacggagccc
     1081 gaaaaggcgg ccgatgagga gatgggcgca tccgctggaa gtgcctccgg tctgcatggg
     1141 gggcggcttg aggggtcaga gtcggaactg gtcgatgggc taagatcgtc cgtttcggta
     1201 tccgtgtctc ctgcggaagt aagtattgaa ggcccagaga cagcggtgtc cagggttgcg
     1261 cgtgctgttg ccatggtggc tgtcggacat gagtcaacag ttgttgtccc ggtgctggct
     1321 ctcgatatcc atggccagac ggtgtctgat gtgatcgtct atccggagac ggtgacagtc
     1381 gatttttact ctaaggcaat atggtaaagc ttgtagccgg cgaatgcggc ctggcgcaag
     1441 agacgggggg ctcaaattgg gcaagctttt tggaacagat ggggtcaggg aggttgcgaa
     1501 tacgggactc acgcctgagc tggcattcag attggggaga gcaggcgcct cgtcagtcct
     1561 gtcccgaagt ggggcgagac cgacgataat cgtcggcggc gatacgcgga gatccactgg
     1621 aatgcttgag tccgcgttgt ccgctggcat aaactcggct ggcgttgatg tactaaatgt
     1681 gggtgttatt ccgacgcctg cagtagctta tttagttgtc aagttcggag cggatgcggg
     1741 aattgtggtg tcggcgtcgc acaatcctgc cgagtacaac ggtatcaagt tcttcggccc
     1801 ggatggcttc aagtttcccg acgaagtcga ggacatcata gagaagaggg cattggcggg
     1861 cgggagcggg gagatgggtt ctgtgttgtg gtcggaggac gatggactgc cccgaccatc
     1921 gggttcgggt ttgggaatgc gcaaagaggc gcctgatgct tgggaacgat atgtggagca
     1981 cgcggttgag tcggccagct gcaggctgga tggattgcgc gtcgttttag attgcgctaa
     2041 tggtgcttca tgcctgacga cgccagccgc ttttcgtgaa cttggcgccg agattatcgt
     2101 cataaacgca tctcccgacg gtgacaatat caactgcgct tgcggatcta cgcatcctga
     2161 cgagttggcg gcggtcgtag tggagcgcgg tgctgatatg ggatttgctc atgatggaga
     2221 cgcggacagg gtgatcgctg tcgatgctga tggacgcatt gtagatggcg atcagataat
     2281 ggcgctatcc gccatccaca ggcattctcg agggggcctt ccggggcggg ctatcgtcgc
     2341 tacccaatac agcaacctgg ggcttactca tgccatggaa tctatcgggt gttctgttct
     2401 cacagctgat gcaggagata gatacgtgct ccagatgatg ctcgagaaga acctcgttat
     2461 tgggggagag caatcggggc atgtcatatt cctggaaaag gcgacgactg gggatgggct
     2521 tattacagcg atagaggtcg caggaatagc tgtggaaaca ggaaaacccc tttcggagtt
     2581 ggccgctgtg atgaccaagt atccgcagat aatggtgaac gtccgcgtaa gccggaagga
     2641 cgactattac cactcagaca cgatagcgga agcggtaaag gcagccgcgg ccgagctggg
     2701 ttcgagtggt cgactcttgg tgcgaccttc gggaactgaa cccgttgttc gggtgatggg
     2761 tgaaggaaag gacgaggcta aagtcgaccg gatagtgcag tctcttgccg agattatcca
     2821 atcggagctc tgctagacgg gctacggcgg gttgaggagt ctcaaggatg tgttgccaac
     2881 gcgtgctcgg tataggagtg gacattatcg aaatccgaag atttcatcgc attgctgctg
     2941 ttgcgcctca aggggtcgcc caaaggcttt acacggcaga ggagctctca gacgggcgca
     3001 ttggtcgtcc cgaatacctc gcctctaggt tcgcggcgaa agaggcggtg atgaaatccc
     3061 tagggtgtgg aatggattcg atagcattca ccgatataga ggtttttcag gatgaatatg
     3121 gacaacctca tgtgaggttg actgggagcg cccgtgcgcg tgcggattct atcggggcgc
     3181 gcacggtcat gatatccctt tcgcattcag acgaaatggt gtgtgctttt gcggtttcag
     3241 tggggggtgg ttgcaacgag ggttgcgact ccagatgagt caagaagaat agacagggac
     3301 gctatcgagc tgtacaaaat agatggaata gttctaatgg agaatgccgg agccgctgta
     3361 gcaagggtag cagttgagat cggagccgag cgtagtggct ggagtgccgg aaggccctct
     3421 gtctgcatat ttgcgggaaa gggtaacaat ggcggcgatg gcttcgttgc ggcgaggcat
     3481 cttgtgggct gtggtgtgga cgtctcagtc tacgtaaccg gatctttgga cggcatatca
     3541 ggagatgccc gcacaaacct ggacatactt aggaatatgg gagttgacgt tgttgggctc
     3601 acttgcgaag aagcttggtc ggcggcggcg gatgatcttg ctgtagccga tgtagcaatc
     3661 gacgccatac ttgggactgg cgctaagggc gcgccttgcg gggagcctgc gcgggccata
     3721 tcaatgatga gcaagtcgcc tgtgcctatc gtggcggtgg atgttccaag tggcgtagat
     3781 gcggggagcg gagctgttgc cggggcctgc gtaacggctc acacaactgt gacatttggg
     3841 ttgcccaaga cggggttgtt gatgtaccca ggagcggcac acgtaggtta tctattggtg
     3901 gacagaatcg gattgccgcc tgcgttgaca gattcgccca aaatcaagac gtgcatagtt
     3961 tgccctgacg atgtgagtgc agctttgccg cagcggaggc ctgatgctca taaaggcgac
     4021 tgcggacgtg ttctggtggt ggcgggttcc cggggaatgg ttggcgctgc ggcgctagct
     4081 tcaatggcgg ctcttcgcgc cggagcgggt ttggtttacc tggctgctcc ggaaggcgtg
     4141 gcagctcagg cgtgtgcagg gctcacggaa gttattgtga ggcctctgcc tgaaacgagt
     4201 tcgggatcga ttgccggaat tgctgtggac agagtcttgg acgaggctgc caagtgcgat
     4261 gctgtagtgg tcggcccggg gctcacttgc tctgacgact cgaggctgat cgtggaggct
     4321 ctgatagcag agggccaaac gcccatggtg attgacgcag atgcgctcaa tctgctttca
     4381 ggtcgggttg acacgcttca gcgggcgtcg gcacctgtcg tgcttacgcc tcaccccggc
     4441 gagatgagcc gactcgtgga ggcaggggtt gaagagataa atgcggacag gctggagtgg
     4501 gctcggcgcg ttgcccatcg ttcaggggcg gtagtcgcac tcaagggcgc ccggactgtg
     4561 acggcgactc cggacgggat tgcctacctg aaccttacgg gcaattccgg catggcaacg
     4621 gctggctcag gagatgtgct ggcaggagtt attggatcat tgatggcgca gggggtccct
     4681 ccgcagtggg cagctgtgtc cggcgtgtat ttgcacggtt tggccgggga ttatgctgcg
     4741 cgtgaagttg gattacatgg aatggtagcg ggtgacatat tgcttcgtat ccccaaggca
     4801 tatcatgata tcgttgagga atctgccggg ctgtgggaca tgatgtcagt aaggagcgcc
     4861 caatacaaga aagaggtggt gaatcctgct gcaaggggtt tatcagcccg gaatgaggcc
     4921 aacgtgggta gaagttaata caggaaacat tgcaaggaac ttgagacaaa tcaaatcgac
     4981 gttacccaag gatacgcaga tactggcggt ggtaaaggca gacggatatg ggcatggagc
     5041 tgctccagtc gccatccgag cgctcgagga gggagtctcc tttttggggg tggccctggt
     5101 tgaagaggga gttgcgttgc gcagggcagg gatagctgcg cccatccttt tcatggagga
     5161 cttgttccct tggcaggcgc ttgacttagt aagatacggg ttgactgcca caattgcgtc
     5221 ctacgagtcg gcggagatgc tttccgaggc cgcaatggcc ctggggaaga aggccaaagc
     5281 acatgtgaag gtcgataccg gaatggggag gcttgggctt ccgccgaacg aaatagccaa
     5341 ccttgtgagg cgtgtggcag agttgcctgg gatcgagata gaggggatct ttacgcacct
     5401 ggcttgtgcc gagtgtaact cggagatgac gggacggcaa cttgagcagt ttgatgacat
     5461 aacagagaag ttgcgcagag agggacatga gatacccatt cgccacgttg caaacagcgc
     5521 tgcgatgctc tctctgccgg aggcggcata cgacatggtg cgcgtaggag gcatcatgta
     5581 tggtctttcg ccgttgggaa ccgttgctcc tgaaaaacca tgggtgcctg cgctttcttt
     5641 ccatactagg gtgtcatatg tcaagctggt tggccctggc acaccggtga gctacggggc
     5701 gacgtatgta acatcaggtc gggagatcat tgctaccttg ccggtaggtt acgcagatgg
     5761 ctatccgcgg gccctctcca acgtcggaga agtgctgata ggcggggaac gatgccctgt
     5821 ggttggcaga gtatgtatgg atcagaccat ggtttcgttg cccgctgatt cggacgtaag
     5881 ggtgggtgac gaagtgacgc tcatcggggc tcaaggtggg gatcggatat tcgccgaaga
     5941 gctggcgaag gctgcgggaa ctattggata cgagatcgtt tgcgcgataa gcaagcgcgt
     6001 gccaagagtc tacgtggcgg gcgatgaata ggagcctaga taagagcatt gcgctgggac
     6061 gggcagaatg cgggctcatt tgctgagctt gcgggcggca ggcatatgtt gggcatggac
     6121 ccttgtttgg atctatcccc agccagaagg ggtaatcgaa ggcttctttc catctgttcc
     6181 ttgggttgac agtgaaatgt gcacgaatat ataattggct tgtatgtgaa catatatcag
     6241 gaagcacaac tcgggtgggg gtgtaggcca tgacacttct tagaaagata gtggtaagca
     6301 tgccggccgg gcttctggaa gaactggatg tgtgtgctag gcagtcaggc cgcaaccgca
     6361 gcgaagttgt gcgcgaggca atgagatcgt acctgtccca gcatgcccgt tccgacctgc
     6421 gtgagaagct gaaacatgga tatattgaga tggcctcgat caatctggag ttggcgcgcg
     6481 aatgtgctgc tgctgaagac gaggcctttg ctgtttctga acgcttcctt gcaggcggtg
     6541 actagctttg gatgtgaaga ggggcgatgt cttctatgcg gatctcaatc cagtggtggg
     6601 gtcggaacaa ggggggctcc gtcctgttct ggtgatccag aacgatatcg gcaactacta
     6661 cagcccgacc acgatagtcg ccgcacttac ttcccgaatc aagcgggcga aacttccaac
     6721 acatgtagaa cttcctgccg ggcagggata ccttgccgtg gactctgttg tgcttttgga
     6781 gcagttgcgc acaatcgaca aaacgcgtct gcatgaccga atctgcactc tcgacgagga
     6841 gattatgaag agggtgaaca gagcggctga gatcagcctg gggcttctgg aaatctgaat
     6901 ctgatctcac cccggccata ttgcgaggcc gggatcatta tgtaccttcg aggtgagctc
     6961 ctgtgccgcc tttgatcttg atttcgagcg actttgagtc cgggaccaac ggcaaaatcg
     7021 ctgtaggagt cgaatacgcg aaggcaatct accggtcggg agggatgcct ttgatcttgg
     7081 cacctccaat gggttgcgcc gactccaaca gagttgccca tggcattctg tccgatgcct
     7141 gtgggctgct gctcacgggc ggagatgatg ttgatcccag gttttatggt gaatcccatg
     7201 ctcatcccaa gataggcgct atcaatcctg tgcgggatga gttcgaaata gctcttgcga
     7261 aagccgcact gaagctcgat atccccgtgc tggggatatg taggggagcg cagatactaa
     7321 atgttgcagc ggggggcgga ctgatccaag atattgccag ctgcgtagat ggtgcccatg
     7381 cgcaccgggt ttctgcgcca aggtggcatc caacccatcg agtcatgctt gctcgcaatt
     7441 ctatattgtt cggaatattt ggagaagaaa caatctgggt aaacagcttc catcatcaag
     7501 ccgtcggtcc tgtagcacca ggattttcgg tgaccggtgt ggctgacgat ggagttcggg
     7561 aggcgataga aaaacccggt tcccgctttg taatgggggt tcagtggcat ccagaaggtc
     7621 tcgcggagag ggatgcgcag tctttgcgca tattcaccga attcgtcaat gctgtacgag
     7681 gagtgtagtg aattgcctac aatgtctgcg ccgctggtgc aagttaccag aggagaactg
     7741 gttgaaagcc tccacagggg ccacatagct gtagttgatt gccgcggcaa tgtgctgtgg
     7801 caggcaggag accccatgta tgttacgtat ttcaggtctt cggctaaacc gttccaagtg
     7861 ttgccaatta tcgagtctgg cgcggttgac cgattcggca ttaccgagag agagcttgcg
     7921 gttatggcgg cctcccatca tggggaagat tgtcatgtgg aagctgtaat gagcattctg
     7981 ggcaagat
//
LOCUS       DAOSAI010000065         7852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5988_length_7852_cov_3.882134, whole genome shotgun sequence.
ACCESSION   DAOSAI010000065 DAOSAI010000000
VERSION     DAOSAI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5988_length_7852_cov_3.882134"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLM83_03625"
     CDS             <1..516
                     /locus_tag="PLM83_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOK70531.1"
                     /translation="GRFETVDEGQDFAVVVDYAHTPDSLANVLATARDISDGRVICVF
                     GCGGDRDRSKRPLMGAIAQRMADYSIVTSDNPRGEDPADIVREIVQGMSEHEAGVEGK
                     AGLEGREKWKIILDRGEAIREAVRCARRGDVVLIAGKGHETYQIFRDETVHFDDREEA
                     RAALRELRMDA"
     gene            509..1915
                     /gene="murF"
                     /locus_tag="PLM83_03630"
     CDS             509..1915
                     /gene="murF"
                     /locus_tag="PLM83_03630"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOK70532.1"
                     /translation="MLDYTLKDVAYAMGGRLAQGDPDQRVSSVVIDSRVAAPDALFFA
                     LPGERVDGHDFLVDAAAGGCCGAVVSRVPVPGQGLPAQFGLIVVDDVLEGLGAFAGRH
                     RMSIDPTVVGVTGSAGKTTTKDMTAAVLAAGFKVIATSGNRNNEIGLPLTLLEMTPEH
                     QIVVVEMAMRGPGEIAALSRIACPDVGIITNIGYAHVGLLGSKENIAAAKAELIEALP
                     PDGFAVLNGDDALVRRMAGLVLGRAWLYGLSEECSVRAESIEPEADSISFSLVIEGHR
                     VDGVRFSVPLPGLHNVYNALAALTVGWRLGLSPDVMATGLASFSPSPMRMEFASSPAG
                     YTVINDAYNANPASVKCAIDAALQYARGRRVILAFGDMLELGETSAVAHEDVGRYAAQ
                     AGVAGMVSVGGMAALAAGAAARSSVEAEACSSNSEALRVLGRMARPGDVVLVKGSRGM
                     QMEEIVGGLLKAPDSGEV"
     gene            1912..2880
                     /gene="mraY"
                     /locus_tag="PLM83_03635"
     CDS             1912..2880
                     /gene="mraY"
                     /locus_tag="PLM83_03635"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOK70533.1"
                     /translation="MMSEVVVRASIAAGIAFAVAMLIGPITIKNLRKLKAGQVVRDDG
                     PQSHLKKSGTPSMGGVIIIVSVLVGSAAASPSYAMLPWALFVTTGCGLVGMLDDYISI
                     VARRSLGLRARQKILFQLVFGLILGAWAYSNPGIGSGIIIPFTHGHVVDLGMWYIPFV
                     MLVLVASTNAVNLSDGLDGLAAGTMAIASFAYVAVSSALGHGEASAFAGAVGGACLGF
                     IWWNAYPASVFMGDTGSLALGAALASMAVITRTELLLLVIGGIYVVEAMSDVIQVTYF
                     KLTGERVFRMAPIHHHFELKGVAEPKIVVRFWIVAAFLAALGFAAL"
     gene            2901..4007
                     /gene="ftsW"
                     /locus_tag="PLM83_03640"
     CDS             2901..4007
                     /gene="ftsW"
                     /locus_tag="PLM83_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOK70534.1"
                     /translation="MLNDRGGLPDLVIFVATLALLSIGIVMVFSASSVTAYHELGDPY
                     YYLKRQSLWAIIGIAAMIMCMNIDYHVWERLAGLLMVLSLGALVLVLVPGVGIVVSGS
                     RRWLGVESIRVQPSEFAKLSLVVFLASYLSASQARTKSFFRGFLLPMMIIGACSVLVL
                     KEPDLGTAIGIAGVGWFMLFAGGSNMASLIATVAAAIPAVYLFARQDPVRLRRLTSFL
                     DPWADPLDSGFHIIQSLLALGSGGLVGVGLGLSRQKFHYLPEQHTDFIFAVIGEELGF
                     LGAASVVLLYMIFGLRGFRTSLNAPDSFGSLLAAGITIMVTLQAMMNIAVVSGMMPIT
                     GITLPLISSGGSSLVPMLAGIGILLNVSKHVNQR"
     gene            4041..5201
                     /gene="murG"
                     /locus_tag="PLM83_03645"
     CDS             4041..5201
                     /gene="murG"
                     /locus_tag="PLM83_03645"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOK70535.1"
                     /translation="MKSEKSAQKRFLLAGGGTGGHIYPAISIARELLSRYPDANILFV
                     GGKRGLEGDLVPKAGFEFQTITVSGFERRLSLDTFATAGRAVAGLFQSIAIVRKFRPS
                     VAVGTGGYVSGPVMLAAAMLGVPVVIHEQNVVPGATNRLLSRWAKTVAVSWEESKQYL
                     ARPERSVLTGNPIRPEIMSAQREESAASFGLDPRRPIVLAFGGSQGARRVNTAFIRAL
                     PSLSGKHDAQFILATGSDKFEESMALAAESGIRASISSDGIGRSDRGDIIIMPYIYNM
                     PQALACADLAICRAGAITLAELAARGVAAVLVPHPHVPDNVQEKNARAMEARGAARVI
                     LDSELEPEVLGREIDSLLSSRPVLASMSRASRNLGRPDAAARVADCVVKAAR"
     gene            5390..6826
                     /gene="murC"
                     /locus_tag="PLM83_03650"
     CDS             5390..6826
                     /gene="murC"
                     /locus_tag="PLM83_03650"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOK70536.1"
                     /translation="MSSMRVHFVGIGGSGMSPLARILIGHGAEVSGSDIRYSPHLEDL
                     AALGADVYQGHDPSRIDGVDLVVASAAVPQDDPELQAAHERGIRVVSRAELLGEIMRP
                     RRGIAVTGTHGKTTTSGMIAHILNEAGLDPTVAVGAGLPWTPSGGHAGEGEFMVVEAD
                     EAYGSFLKLDPEIAVVTNIDDDHRDYYETFEGIMEGFTQFLRRIKPGGFAVLCADDHN
                     VLRAAEGLDRRIIYYGISSEADYTSADYSQLGFGSRFSVMRRGACLGQIELSVPGMHN
                     MSNAIAAVAVCDQLGIDFSIISRGLAGYMGAERRCQVLAKTPEMTLVDDYAHHPVEIR
                     ATLAALRKAAEGRLVVVFQPQRYTRTKYLRDEFARSFSLADVIAITDIYYEGTGESPI
                     PGVSGRDLAERIAAYEGKPVTYLADRAALDDFLRNSTGPGDTVVTMGAGDIYRSSRRF
                     AEELARPEAGFRLSHDGRWKLDDEHGHR"
     gene            6804..7721
                     /gene="murB"
                     /locus_tag="PLM83_03655"
     CDS             6804..7721
                     /gene="murB"
                     /locus_tag="PLM83_03655"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOK70537.1"
                     /translation="MTSMVTDEQARQLAKSFNGRIALNEPMKKYTSFQIGGPADMLVQ
                     PRDLQSLSVVLRWAWNEGLPLFVLGNGTNLLVADEGIRGVVVRIGPGLDSVSFDGERV
                     RAMAGVSMPALARACAERGLAGLEWAVGIPGSLGGAMAMNAGAYSHTVGDFTREVTTM
                     LLDGSISVKSCEEMQFAQRSSRLSHGDLIACEAVLVLEPGDPESIRCQMSEYMEDRRI
                     KQPLHLPSAGCVFQNPSGRGAGRYIDGAGLKGLRVGGAEISRVHANFIVNVGDATARD
                     VLILMNVARRTVYEKFGVELVPEIRVVGG"
BASE COUNT         1591 a         1855 c         2547 g         1859 t
ORIGIN      
        1 ggaaggttcg aaactgttga cgagggccag gattttgcgg tcgtcgtcga ttatgctcac
       61 acccccgatt ccctcgcgaa cgtgctagcg accgcccgtg atatttccga tggaagggtc
      121 atatgcgtct tcggatgtgg cggcgacagg gatcgatcga aacgtccgct gatgggcgcc
      181 atagcgcaga ggatggctga ctattccata gtaacgtctg ataacccccg cggtgaagac
      241 ccggcagaca ttgtcaggga gattgtccag gggatgtcgg agcatgaggc cggcgtcgaa
      301 ggcaaggcag ggctggaggg gcgggaaaaa tggaagataa tcctcgaccg cggggaagcc
      361 atacgcgaag cagtgcggtg tgcccgccgt ggcgatgtag tgcttattgc agggaagggg
      421 catgagacat accagatctt ccgggacgag acggttcatt ttgatgacag agaagaagct
      481 cgtgcggcct tgagggagct gaggatggat gcttgattac acgctcaaag atgtggccta
      541 tgccatgggg gggcgcctgg ctcagggaga cccggatcag agggtgagct cggtcgtgat
      601 tgacagcaga gtggcggcac cggatgcgct tttctttgcc ttaccaggcg agcgagtcga
      661 tggacatgat ttccttgtag acgcagctgc tggcggatgc tgtggcgcag tggtgtcgag
      721 ggtgcccgtg cctgggcagg gtttgccagc tcaatttggg ctcatagtag tcgacgatgt
      781 tcttgaaggc cttggagcct ttgcagggcg tcacaggatg agcattgacc caactgttgt
      841 gggggttacg ggcagcgctg ggaagacgac aacgaaggat atgacggccg cagtgttggc
      901 ggcgggattc aaggtgattg ccacctcggg caataggaat aacgaaatcg gtctgccgct
      961 gacgctgctg gaaatgacgc ctgaacacca gatagtcgtg gtcgagatgg cgatgcgcgg
     1021 ccctggggag atagctgcgc tatcgaggat tgcatgccca gatgtgggca tcataaccaa
     1081 tatcggttac gcgcatgtgg gccttttagg atcgaaggag aacatagctg ccgcaaaggc
     1141 cgagctgata gaggcgctcc ctcccgatgg gtttgcggtt ctcaacggcg atgatgctct
     1201 tgtcaggcgt atggcagggt tggttttagg ccgcgcctgg ctctatggcc tgtctgagga
     1261 atgctctgtg agagcggaga gtatcgaacc ggaggccgac tcgatcagtt tcagccttgt
     1321 tattgaagga catagagtgg acggagtgcg tttttcggtt ccgcttcccg gcctccataa
     1381 tgtgtataat gccctggcag ccctcaccgt gggctggcga ttggggctca gtcccgacgt
     1441 aatggcaacg ggccttgctt cattcagccc ttccccgatg agaatggagt ttgcgtcatc
     1501 tcctgccggc tatactgtga taaacgatgc ctacaatgcc aatcctgcat cggtaaagtg
     1561 cgcaatcgat gccgcgttgc aatatgcgag ggggcgtaga gtgattctgg cctttggtga
     1621 catgctcgag cttggcgaga ccagtgcggt tgcccatgag gatgtgggca ggtatgcggc
     1681 gcaggcaggc gttgccggaa tggtgagtgt gggcggaatg gctgctcttg cagctggggc
     1741 tgcggcgcgt tcttctgtgg aggcagaggc atgtagcagt aactcggagg cgttgcgagt
     1801 tctcggaaga atggcccgtc cgggggacgt cgtattggtc aaggggtcaa ggggcatgca
     1861 gatggaagag attgtgggcg gccttctgaa ggctccagac agtggtgagg tatgatgtcg
     1921 gaagttgtgg ttcgcgcatc gatagcagca gggattgcat tcgctgttgc catgttgata
     1981 ggtccgataa ccataaagaa tcttcggaaa ctcaaagcgg gtcaagttgt tcgggatgac
     2041 ggaccccagt cgcacctgaa aaagtcgggc acgcccagca tgggcggggt gattatcata
     2101 gtctctgtac ttgtgggttc agcagcggct tcgcccagtt atgctatgct gccatgggcg
     2161 ctttttgtta caacgggctg tgggcttgtg ggtatgcttg atgattacat ttcgatcgtc
     2221 gctcgtcgtt ctttgggctt gcgtgccagg cagaagatcc tgtttcagct ggtgttcggg
     2281 ttgattttag gggcatgggc ttattcgaat ccagggattg gatctggcat tatcatcccg
     2341 tttactcatg gacacgtggt tgatcttgga atgtggtata taccctttgt catgctagtt
     2401 ctggttgcct cgacgaatgc cgtcaacctg tcggacggcc tcgatgggct tgctgcaggg
     2461 accatggcaa tcgcttcatt cgcgtatgtc gctgtatcat cggcgctcgg ccacggagaa
     2521 gcaagtgcct tcgcaggcgc tgtgggcggg gcgtgcctcg ggttcatctg gtggaacgct
     2581 tacccggcat ctgttttcat gggcgatacc ggatcattgg cattaggcgc tgcgctcgct
     2641 tccatggcgg tgattactcg gacggagctc ctcttgctcg tgataggcgg gatatatgtt
     2701 gttgaagcca tgtccgatgt catacaggta acctacttca agctcaccgg cgagcgtgtg
     2761 tttcggatgg ctcctatcca ccaccatttc gagctgaagg gagtggcgga accgaagatt
     2821 gtggtgagat tctggatagt ggctgcgttt cttgcggctt taggatttgc ggcgctttag
     2881 gcgtacgggg tgattttggc atgttaaacg accgcggagg gcttcctgat cttgtcatct
     2941 tcgtcgcaac gctggctttg ctcagcattg ggattgtgat ggtcttcagc gcgagctcgg
     3001 taacggccta tcacgagctt ggagacccct attattatct gaagcgccaa tccctctggg
     3061 cgataatcgg aattgcagca atgattatgt gtatgaacat agattaccat gtatgggaga
     3121 ggcttgcagg ccttcttatg gttctttcac tgggagcgct tgtcctcgtt ctggtgcccg
     3181 gggttggcat tgtcgtgtca gggtctcgaa gatggttggg agtcgagtcc attcgggtgc
     3241 agccttccga attcgcaaag ctgtcattgg tcgtgttcct ggcgtcgtat ctttcggctt
     3301 cccaggctag aacgaagagc ttctttcggg gtttcttgct gccgatgatg attatcgggg
     3361 cttgctctgt tcttgttctg aaagaaccgg accttggcac agcgattgga atcgcgggag
     3421 tgggatggtt catgctcttt gccgggggat cgaacatggc atcgctgatc gccaccgtag
     3481 ccgcggcgat tcccgccgta tatctttttg caaggcagga ccctgtaagg ctcaggagac
     3541 tgacttcgtt tcttgatccg tgggccgacc ctcttgattc cggattccac ataatccagt
     3601 ctcttctcgc actgggctcc ggagggcttg tcggcgttgg actggggctg tcgaggcaga
     3661 agtttcatta ccttcccgag caacacaccg acttcatctt cgccgtcatc ggagaagaat
     3721 tggggttttt aggggcagct tccgttgttc tcctttacat gatatttggc cttcggggtt
     3781 ttcgaacatc gctcaatgcc cctgactcgt ttgggtcgct tcttgctgct ggcattacaa
     3841 taatggtcac tttgcaggct atgatgaata tcgccgtggt ttccggaatg atgcctatta
     3901 ccggaatcac gttgccgctg ataagttcgg gtggctcgtc gctcgtgccg atgttggcgg
     3961 gcataggaat actgctcaat gtatcgaagc atgtcaacca gagatagggt agggttctct
     4021 aggtcggggg aggtagctcg ttgaagagtg agaaatcggc gcagaagaga ttcttgttgg
     4081 cgggaggcgg cacggggggc catatatacc ccgccatctc catagccagg gagttgcttt
     4141 cgcgatatcc tgatgccaat attctgtttg tagggggaaa gcgcggcctg gaaggggatc
     4201 tcgttcccaa ggcgggtttc gagttccaga cgatcaccgt ttcgggattc gaaagaaggc
     4261 tctcactcga cactttcgct accgcaggac gcgcggtggc aggccttttc caatccatag
     4321 ctatcgtgag gaaattcagg cccagcgttg ccgtggggac agggggatac gtttcgggcc
     4381 cggtgatgct tgcggcggcg atgctagggg tgcctgttgt tatacatgag cagaatgtgg
     4441 ttccgggtgc taccaaccgc ttgctttcac gatgggcgaa gacagtggcc gtgagctggg
     4501 aagagtcgaa acagtatcta gctcggcccg aacgttcagt ccttaccggg aacccgattc
     4561 gtccggaaat aatgtcggcg caacgagagg agtcggcggc atcgtttggg ctggatccga
     4621 ggcgtccaat tgtgttggcg tttggcggga gccaaggcgc caggagggta aatacagctt
     4681 ttatccgagc tcttccgtcg ctatcgggga agcacgacgc acaattcata ctggctacag
     4741 ggagcgataa attcgaggaa tctatggcgc ttgcggctga atcaggcatt agggcaagta
     4801 tttcctctga cggaataggc aggtcggatc gtggggacat tattattatg ccatacattt
     4861 acaatatgcc ccaggccctt gcctgcgccg acctggcgat ttgccgggca ggggcgatca
     4921 cccttgctga acttgcggca cggggcgtgg ctgcagtgct cgtccctcat ccacacgtcc
     4981 ccgacaatgt gcaggagaaa aatgcccgcg ccatggaagc caggggtgct gccagagtga
     5041 tactcgacag cgagcttgaa ccggaggttt tgggacgtga aatagactcg cttttgtcgt
     5101 caaggcctgt actggccagc atgtccaggg cttcgcggaa tctgggaaga cccgatgctg
     5161 ctgcgagggt ggcagattgt gtggtaaagg cggcgcgata gacggcacat tacgataaga
     5221 tcaaaccttg aatgtgattt gtccggaggc atcgtggtcg actcattcag catctgggcg
     5281 cgccgcagca agatgcattc tatgggcagg acatgctctc cgagggggca gaaggcatac
     5341 aatagcatcg tccgcgggag cgtggcgtgt cgggaaaggt gcgggtcata tgagttcaat
     5401 gcgcgttcat ttcgtgggaa taggcggcag tggcatgagt ccgcttgcca gaatactcat
     5461 tggccatggg gccgaggtga gtggatcgga tatacgctac tctcctcatc tcgaggatct
     5521 ggcggctctt ggcgctgatg tatatcaggg tcatgatcca tcacggatcg acggggtcga
     5581 cttggttgta gcgtcggctg cagtgcctca ggatgatccc gaacttcaag ctgcgcacga
     5641 gcgtggaata agggttgttt cgagagcaga gctcttgggc gaaatcatga ggcctcgacg
     5701 gggaatagcc gtaacaggaa cacacggaaa gactacgaca agcgggatga tcgcgcatat
     5761 tctcaacgaa gcgggcctcg atcccacggt ggcagtagga gccggtctgc catggactcc
     5821 gagcggaggc catgcaggcg agggggagtt catggtagtc gaggcggatg aggcgtatgg
     5881 atccttccta aaactcgatc cggaaatcgc tgttgtaacc aacatcgatg atgatcatcg
     5941 ggattactac gaaacatttg aaggaataat ggagggtttt acccaatttc tccgaagaat
     6001 taagcctggg ggatttgcgg ttctgtgcgc tgacgaccac aacgtgttga gggccgctga
     6061 gggacttgac cggcgaatca tctactatgg catctcttcc gaggccgatt acacttcggc
     6121 cgactattcg cagctcggct tcggatcgcg tttttctgtt atgcgccgcg gagcatgcct
     6181 cgggcagatt gagctttccg tcccgggaat gcataacatg agtaatgcca tagctgccgt
     6241 tgccgtctgc gatcagctgg gaatagattt ttcgatcata agcagggggc ttgccggtta
     6301 catgggtgct gagcgcagat gccaggtgct ggccaaaacg ccggagatga cattggtgga
     6361 cgattatgcg caccatcctg tggaaatcag ggcaactctg gccgctctgc ggaaggcagc
     6421 cgagggacgg cttgtggtcg tatttcaacc gcaacgctac acaagaacga agtatcttcg
     6481 cgacgagttt gcccggtcgt tcagtcttgc cgatgtcata gccattaccg atatctacta
     6541 tgagggcaca ggtgagtcgc ctataccggg agtttccggg agagaccttg cagagaggat
     6601 tgcggcgtat gagggaaagc ctgtgaccta tcttgccgac agggctgctc tggatgactt
     6661 tctcagaaat tcgactggcc ctggcgacac agttgttacc atgggagcgg gcgacatcta
     6721 cagatcgtca cggcgcttcg ccgaggagct ggctcgtcct gaggctggct ttaggctatc
     6781 gcatgatggg cggtggaaac ttgatgacga gcatggtcac cgatgaacaa gcaaggcagc
     6841 ttgccaagtc gttcaacggg agaattgcgc tgaacgagcc aatgaagaaa tatacgtcgt
     6901 ttcagatcgg tggtccagcc gatatgcttg tgcagcctcg cgatctgcag agcctgtctg
     6961 tggtattgcg atgggcctgg aacgagggat tgcccctttt tgttctgggc aatggtacga
     7021 accttctagt tgcggacgaa ggaattcggg gcgtagtagt gaggataggt ccgggcctgg
     7081 acagcgtcag ttttgacggc gaacgggttc gcgcaatggc aggcgtcagc atgccggctt
     7141 tggcccgtgc ctgcgccgag cggggccttg ccgggctgga atgggcagtg ggaataccgg
     7201 gttcactcgg cggggcaatg gcaatgaatg ctggcgcgta cagtcacact gtgggagact
     7261 ttactcgcga agtgaccacc atgttgcttg atgggtcaat ttcagtgaag tcatgcgagg
     7321 agatgcagtt tgcccagagg tcgagtcggc tttcccacgg agatctcatc gcctgcgagg
     7381 ccgtgctcgt gttggaaccg ggcgatccgg aaagcatacg ttgccagatg agcgaataca
     7441 tggaggatcg cagaataaag cagcctctgc accttccgag cgccgggtgt gtattccaga
     7501 atccttcggg gagaggggcc ggtcggtaca tcgatggagc gggtctcaaa gggcttcgcg
     7561 taggcggggc agagatatcc cgagttcatg caaacttcat agtgaacgtg ggcgatgcga
     7621 ctgcgcggga cgtgttgata ctgatgaatg tggcgaggcg gaccgtttat gagaaattcg
     7681 gagtcgaact tgttcctgag atcagagtag tcggtggtta ggcctggtgt cgttatagat
     7741 taagacgcgc gaggtggctg agatggagac tttgaggata gtcggcgggc gtgaactgag
     7801 aggcagcata gcgacgccgg gcgcaaagaa ctcctgcctg aaactgcttg cc
//
LOCUS       DAOSAI010000066         7851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_5989_length_7851_cov_3.716521, whole genome shotgun sequence.
ACCESSION   DAOSAI010000066 DAOSAI010000000
VERSION     DAOSAI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5989_length_7851_cov_3.716521"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PLM83_03660"
     CDS             complement(<1..1307)
                     /locus_tag="PLM83_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOK70538.1"
                     /translation="MLSEIDVRNYALIERLSLRFGKGFNVLTGETGAGKSLVIDCVGL
                     VIGGRASSESVRAGCDSAVVEAVFNISDVPDVEALLLEQGIDPEPDGTLIVTREVSRQ
                     GRSRCRLNGRIVTLTALSAIGERLVDIYGQHEYQSLARPSRHIGLLDAFGGARLEQVR
                     DSVRQLYEEWRECEEELAELRQHARERAQRADLLAFQVNEIESANLEPGEDVHLVAEQ
                     TVLANAESLYDAVVRAYSLIYESSYPDIASARDMLGEALEELDNASRIDGTLTSLAES
                     LSALCDHTTEISRALREYRDSIEFDPERLAEVEERLSAISRLKRKYGDSLADVIDHGQ
                     LAREELESMTGAGRRVDELEGRSAALSEELGSCASKLSAMRCEIAAELEKEVSRELRD
                     LSMPHAVFKVDVGRKRDGHEIVVDGTPVVIGPDGFDDVEFLFSA"
     gene            complement(1369..1818)
                     /gene="argR"
                     /locus_tag="PLM83_03665"
     CDS             complement(1369..1818)
                     /gene="argR"
                     /locus_tag="PLM83_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOK70539.1"
                     /translation="MKARRHQRILDIVRSQTIKTQEELAEELAREGITVTQATISRDI
                     KELRLIKAPVGDGTYRYTIPTDRSIEDINRRVERIFREVIISIEDSENIIVIKTLEGA
                     AQAVAAVLDDLAWREIVGSLAGDDTILLIIKPVQSVPDVMSRLNKFR"
     gene            complement(1862..2701)
                     /locus_tag="PLM83_03670"
     CDS             complement(1862..2701)
                     /locus_tag="PLM83_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOK70540.1"
                     /translation="MKGGGLTKRAGIVVHAAKPEARRLAGHVEEWMRGAGVDVVIDDC
                     LDRPLPEDIDCAIVLGGDGTLLSAARRAATRGIPILGVNLGNLGFLTEVEPSQLEPAL
                     RALVDGNYTVEERTMVEAVVFRNGQAVKTLCGLNDVVITKGAFARIVQLGVFVNDAYF
                     ATFPADGVIISSPTGSTAYSLSAGGPLINPNIDVLLITPICPHSLFSRSLVIAGSDKV
                     RVVVETAHEDVAVTVDGQIGFELQSMDEIVVGRAPYVTRFVRLETRNFYGILRERLKR
                     DRL"
     gene            complement(2762..3670)
                     /locus_tag="PLM83_03675"
     CDS             complement(2762..3670)
                     /locus_tag="PLM83_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOK70541.1"
                     /translation="MSQQAKIRLDQLVVERGLAASREAGKREILAGNVFVDGVREDKP
                     GTMIRADAKISLERRGPRYASRGGEKLAHALKTFAINVNGRVAIDIGASTGGFTDVLL
                     KAGASRVYCVDVGYGQLAWSLRQDPRVEVFDRTNARYITPDMFDPRPCFASVDVSFIS
                     LSKILPALYAVLQGRAEAVCLVKPQFEAGRRNVGKRGVVRDPAVHAEVLRAVYGHARD
                     AGFEVLEYTWSPITGPEGNIEFLVHLRKQDANTQRICSCPHAGADAPTSCSLDRELCG
                     IAEAQLDRAIKSTVQQAHVAHGRSGD"
     gene            complement(3654..5573)
                     /gene="dxs"
                     /locus_tag="PLM83_03680"
     CDS             complement(3654..5573)
                     /gene="dxs"
                     /locus_tag="PLM83_03680"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOK70542.1"
                     /translation="MNSILQSIDSPSDLRRLSDREIRQLAEEIRHYLVHTVSRTGGHL
                     APNLGVVELTIAIHLALDCPRDKVIWDVGHQSYVHKLLTGRRGAFASLRQQGGISGFP
                     KPSESEYDAFATGHSSTSISAALGMALARDAAGENYTVMAVIGDGALGGGMALEAMNH
                     AGHTKTDLIVILNDNEMSISRSVGALAGVLGSARTHPGRRRFKYGFDRFVMGIPVIGK
                     ALKAAGYRLKKSVKSFVLPGMVFEELGFTYLGPIDGHNVKAVSRAIDDAKRAGGPVLI
                     HVITRKGKGYAPAEQDPAKFHGTGPFVVETGTQISSDRPTFTSHFSEFMLRAAEEDDR
                     IVAITAAMAEGTGLRSFAEAYPGRFYDVGIAEQHAVTFAAGLATAGLRPVVAIYSTFL
                     QRAYDQIVHDVALQGLPVILAVDRGGIVGEGGPTHQGLLDVGYLRQIPGMTVMAPKDG
                     AELQSMFGTALEHDGPSAIRYPKSACPEREVEWPARPIPIGKAEVMRDGLHCAIFALG
                     PMVWPALEASRILSTRGVSCAVVNPRFVKPIDVDTLLEYAGRTGIIITCEDSCLAGGF
                     GSAVLESLSEAGVFDVHVVRLGAPDDFVGQGKRDDVLSDLGLDAKGIADAVMQALRGR
                     EGERAVGGGLYVSTS"
     gene            complement(5651..6235)
                     /locus_tag="PLM83_03685"
     CDS             complement(5651..6235)
                     /locus_tag="PLM83_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70543.1"
                     /translation="MPRDFGRIIAVGLWTFALSAALSYLSGSAAGNLSPWFSVAVLMA
                     IIATGILFDIVGTAAAAASEAPINAMAANRVYGASEALRLVRNSPAVASICNDVVGDI
                     CGTVSGAVGATIILRIAASTGGWLAESASVLVIALISAVTVAGKAIGKRVAIESANDV
                     VLSVGRIIATYERFTGRSKDAKGRKRNNGRSSKD"
     gene            complement(7143..7607)
                     /locus_tag="PLM83_03690"
     CDS             complement(7143..7607)
                     /locus_tag="PLM83_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOK70544.1"
                     /translation="MFAPDTGLFSNRILWTSILAWAIAQTLKVFTCIWAEGKPDWSKI
                     VGPGGMPSSHSAFVTSIAVGVGIQEGWNSALFAVTVGFALVVMYDAAGIRRAAGEHAQ
                     VLNQLIERAFREGYLESTKLRELLGHTPLEVAMGAFLGIVVSWLMIGWALKG"
     gene            complement(7618..>7851)
                     /locus_tag="PLM83_03695"
     CDS             complement(7618..>7851)
                     /locus_tag="PLM83_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOK70545.1"
                     /translation="IAADIGLAFQIRDDLEDADSQRDSGRSGFLTAVSVWGIDGARRQ
                     FAALMDSARTKCAVLGDAASELTDIIDIVDREW"
BASE COUNT         1790 a         2483 c         1884 g         1694 t
ORIGIN      
        1 gctgaaaaca gaaattccac atcgtcaaag ccgtcaggac cgattacaac gggcgtgcca
       61 tccacaacta tctcgtgtcc atcccttttt ctgcctacat caactttaaa gactgcatgg
      121 ggcatggaca ggtcccgcag ctccctggag acctccttct caagctcggc cgctatttcg
      181 catctcatcg cagagagctt ggaggcgcag ctgcccaact cctccgagag ggccgctgaa
      241 cgaccttcca gctcgtccac gcgacgccct gctcctgtca tggactccag ctcctcacgg
      301 gcaagttgcc cgtggtctat aacatctgcc agcgaatcgc cgtacttccg tttcaacctc
      361 gaaattgcgg aaagcctctc ttccacctcg gcaagtcttt ccgggtcgaa ttcgattgag
      421 tcgcggtatt ccctgagggc gcgggatatt tctgtggtgt ggtcgcacaa tgcagacagg
      481 ctctcggcaa gagaagtcaa tgtcccgtcg attcgagacg cattgtccag ttcctcgaga
      541 gcctcgccca acatgtcgcg ggctgatgcg atatcaggat aagaggattc atagatgagg
      601 ctgtaagcgc gaacgacggc gtcgtaaagg ctttcagcgt tggctagtac tgtctgctcc
      661 gcgacgaggt gaacatcctc gccgggctcg agattggcag attctatttc attgacttga
      721 aaggcgagaa gatcggcgcg ctgtgcgcgc tcccgagcat gctggcgcag ctcggccaat
      781 tcctcctcgc attcgcgcca ttcttcataa agctggcgga ctgaatcccg aacctgctcc
      841 aaccgggctc cgccaaaggc gtcgagcagg ccgatatgcc gggatggtct ggcgagagat
      901 tggtattcat gttgaccata tatgtcgacc aagcgctcgc ctatggccga cagcgcggtc
      961 aaggtcacaa tgcggccatt cagcctgcaa cgtgaacgcc cttgcctaga aacttcccga
     1021 gtcacgatga gcgtcccatc aggctcagga tctatgccct gttcgagcag gagcgcttcc
     1081 acatcgggca catccgatat attgaaaaca gcctccacca cagcagaatc gcagccggca
     1141 cggacagatt cagaggaagc ccttccacca atgacaaggc ccacacagtc tataaccagc
     1201 gattttccgg cgccggtctc ccctgtgaga acattgaatc cctttccgaa tctcagtgac
     1261 agccgttcta taagcgcgta gttccgcaca tctatttccg aaagcatcgc cttgccccca
     1321 aagacgcggc gaccagcaac agcctgctca cacaaccggc ctgccctatt atcggaactt
     1381 gttcagccgg gacatcacat cgggcacgga ctgaaccggt ttgataatga gcaatattgt
     1441 gtcgtcgccc gccaaagacc ctactatttc ccgccatgcc aggtcatcca ggaccgcggc
     1501 aaccgcctga gcagcgcctt ccaatgtttt tatgactata atgttttcgc tgtcttcaat
     1561 gcttattata acttcgcgga agatgcgttc tacccgcctg tttatatctt ctatgctgcg
     1621 atcggtcggg atcgtgtacc gataagtacc gtcgcccaca ggcgctttta tcagccggag
     1681 ttcttttatg tcgcgcgaga ttgtggcctg cgtaacagta ataccctctc gcgcaagttc
     1741 ctcagccaat tcctcctgag tctttatcgt ttgcgatctg acaatatcca gaatcctctg
     1801 gtgtcttctt gctttcacct ccgacgcctc ccgcacatac cttttcccct gcggcccgcc
     1861 attacaagcg gtcccgcttg agcctttccc tcagtatccc gtaaaaattc ctggtctcaa
     1921 gcctgacaaa cctcgtcacg tacggagccc tgccaaccac tatttcgtcc attgactgga
     1981 gttcgaaccc gatctgaccg tcgacggtaa ccgccacgtc ttcatgggcc gtctccacca
     2041 caacccgcac cttatcgctc cctgcaatca caagagaacg cgagaaaagg ctgtgcggac
     2101 agattggagt gatgagaaga acatcgatgt tgggattgat cagaggtccg cctgccgata
     2161 gtgagtaggc agtggatcct gtagggctcg atatgataac cccgtccgcg gggaaagtgg
     2221 cgaaatatgc atcattcacg aataccccaa gctgaactat tctggcaaaa gcccccttgg
     2281 taatgaccac gtcgttaagg ccgcaaagag tcttgacagc ctgtccattg cggaaaacga
     2341 cagcctcaac cattgttcgc tcttcgacgg tgtagtttcc atcaacaaga gctcgaaggg
     2401 ccggttcgag ctgcgatggt tccacctccg tgagaaaacc aaggttcccc agattaaccc
     2461 ctaaaatcgg aattcccctg gttgcagccc gcctagcagc agagagaagg gttccatctc
     2521 ctccaaggac aatagcgcaa tcgatatctt ccggaagcgg tcgatccaga caatcgtcga
     2581 tgaccacgtc gacccctgcc ccacgcatcc actcctctac gtggccagcc agccttcttg
     2641 cctccggttt tgccgcgtgc actacgattc ccgccctctt cgtaagaccc ccccctttca
     2701 tcagtccaga cccgaagccc tatctacagc caatgaccgc gcggacacgt tgctgttggg
     2761 gctagtcgcc gcttcggccg tgggccacgt gcgcctgctg aacagttgat ttgattgccc
     2821 tatcgagctg agcttcggct attccgcaca attccctatc gagactgcaa gaagtcggtg
     2881 cgtcggcgcc tgcgtgggga caactgcaga tccgctgcgt gttcgcgtct tgtttgcgca
     2941 gatggaccaa aaactcgatg tttccctcag gtccagtaat aggagaccac gtgtactcca
     3001 acacctcaaa acctgcatcc cgtgcatgcc cataaaccgc ccgaagaact tcagcatgca
     3061 ccgcagggtc acgaactacg cctcgcttgc caacgttgcg gcgccccgcc tcgaactggg
     3121 gcttcaccaa gcacacagcc tcggcccgtc cttgcagcac cgcatacaag gcgggcagta
     3181 ttttggacaa agatatgaag gacacgtcta cagaggcaaa acaggggcgc ggatcgaaca
     3241 tatcaggcgt tatgtagcgc gcgttcgtgc gatcaaagac ttccacgcgc gggtcctgtc
     3301 tcaggctcca ggcaagttgc ccatagccca catccacaca ataaactcgc gacgcccccg
     3361 ctttcaagag cacgtcggta aatccgcccg tagaggcgcc tatatctatg gcaacgcggc
     3421 catttacatt gatagcgaac gttttcagcg cgtgcgccag cttctctccg ccccgactcg
     3481 cgtaccgcgg gccacgccgt tccagggata tcttggcgtc agcccgaatc atcgtccccg
     3541 gcttgtcctc gcgcactccg tccacaaaga cgtttccggc tagtatttca cgctttcctg
     3601 cctctcggct tgctgccagc ccacgctcta caactagctg atcgagtctg attttagctt
     3661 gttgagacat acaaaccacc gccaaccgcc ctctcgccct ctcgccctct tagcgcctgc
     3721 atcaccgcat cagctatgcc tttggcatcc aaaccgagat cagacaagac atcatcccgt
     3781 tttccctggc ccacaaagtc gtctggcgct ccgagcctca caacatgcac atcgaaaacg
     3841 cccgcctctg acagcgactc cagaactgct gaaccaaacc cacccgccag acaagagtcc
     3901 tcgcatgtga taatgattcc cgtcctgcca gcgtattcca gcaatgtatc aacatcgatc
     3961 ggcttcacaa acctgggatt caccactgca caacttactc cacgggtcga cagaatcctg
     4021 ctagcttcga gagcaggcca taccatgggc ccaagggcga atatggcaca atgcaagccg
     4081 tcccgcatga cttccgcctt gccaatggga ataggccttg caggccactc gacctccctc
     4141 tccgggcaag ccgacttagg ataccttatg gccgatggcc catcatgttc cagagcagtc
     4201 ccgaacatgc tctgcagctc cgcaccatcc ttgggcgcca tcacagtcat cccgggtatc
     4261 tgcctcagat atcctacatc aagcaggcct tgatgagtgg ggccgccttc gcccactatc
     4321 cctccgcggt caacagcaag aattaccggc agaccttgca atgccacatc gtgcactatc
     4381 tggtcatagg cccgctgaag aaaggtggaa tatatggcca caacgggacg gagaccagct
     4441 gtagcaagcc cagcagcaaa agtgaccgcg tgctgctcag caatgcccac atcgtaaaag
     4501 cgcccgggat aggcctccgc aaaactgcgc agaccagtgc cctcggccat ggcagccgtt
     4561 atcgccacaa tcctgtcatc ttcttcagca gcgcgaagca tgaattcgct gaagtgcgac
     4621 gtaaaagtgg gccggtcact ggaaatctga gttccggtct caacaacgaa cggaccggtg
     4681 ccatggaatt tcgcggggtc ctgctcagca ggcgcatagc ccttgccttt tcttgttatg
     4741 acgtgtatca agaccggccc acccgcgcgc ttggcgtcgt ctatggcgcg gctcacggcc
     4801 tttacattgt gtccatcgat tggccccaga taagtgaagc ctagctcctc aaagaccatt
     4861 ccaggcagga caaagctctt gacgcttttc ttcaacctgt atccagctgc tttgagtgcc
     4921 ttcccaatta ccggtatgcc catgacaaac cggtcaaatc catacttgaa gcgtcgtctg
     4981 cccggatggg ttcgagcgga gcccaacacc cctgccagcg ctccgacact tcgggaaatg
     5041 gacatctcgt tatcattgag tatgactatg agatcggtct tggtgtgccc ggcatgattc
     5101 atcgcttcaa gagccatccc gccgccgagc gcgccgtctc caattaccgc cattacagta
     5161 tagttttcac ctgcagcatc acgcgccaag gccatgccga gggcggcaga tatggacgta
     5221 ctggagtgtc cggtggcaaa ggcgtcgtat tcgctctctg acggtttcgg gaaaccgctt
     5281 atgcccccct gctgccgcag agaggcaaaa gccccacgcc ttccggtgag aagcttgtga
     5341 acatatgact ggtggcctac atcccatatc accttgtcgc gaggacaatc gagagcaagg
     5401 tgaattgcta tcgtaagctc aactacaccc aggtttggcg ccaagtgccc gccggtgcgg
     5461 gaaacagtat gtaccaggta atgccgaatc tcctccgcaa gctgccgtat ctccctgtct
     5521 gagagccttc gcaggtcgga aggtgaatcg atgctctgga gaatgctgtt catagggaac
     5581 ccaccgtccc ttgaccgtcc gctccacatt tgggaggatc ggcaataatc attgatgctt
     5641 cagcgtgccg tcaatccttg ctacttcttc cattgttccg ctttctgcct ttggcatcct
     5701 tggaccgtcc agtaaacctt tcgtatgtcg caattatccg ccccactgac aacacgacat
     5761 cgttggctga ttctatcgct acacgcttgc caatggcctt gcctgcaact gtgacagccg
     5821 aaatcagagc gatgaccaac acagatgcgg attctgcaag ccatcccccg gtcgaagcag
     5881 ctatgcgcag aatgatcgta gcgccgaccg ctccgctcac agtgccacag atgtcgccga
     5941 caacgtcatt gcagatacta gcaactgccg gagagttccg gacgagcctt agcgcctcac
     6001 ttgctccgta cacacgatta gcggccatag cgttgatcgg agcttcgctg gccgcggctg
     6061 cagcagtccc cacaatatcg aagagaatcc cagtggcaat tattgccatc aaaacagcga
     6121 ccgaaaacca ggggctgagg ttcccagccg cagagccaga aaggtagctc aaagccgccg
     6181 atagggcgaa tgtccatagc cctactgcta tgattcgccc aaaatcacgt ggcaagtcat
     6241 gttcacctca aaaaatatgg gcttcagcct gaatgcgcat aatatggatc cgaaatagtc
     6301 ggatgaataa ataaaccgat aggtggcagc gccctgagta aatattgtga cttgaggtcc
     6361 agcaggtttt cccccctgtg cgccctaccg tcgccggcgg gcggtttccc tttacgacag
     6421 gttctcctcg tcgccgaagg agcaactgga ttgccactta gatcacaccg taatccccag
     6481 agcgccatgc aatgcaaaca gcctaggagt tttcctcggc aatgctggcc attctctatc
     6541 ctcttttgca gatgggcttt caagcggcaa aaggtcatgt tccttggccg gggagacaat
     6601 gacttggcac ccgccatccc gcacatccac tcaaggcagg ctacgctgca cccagcacca
     6661 ttgggcccac cagggccagt accgacatgc aggttcaccc caagccgtaa agcttttcaa
     6721 ccccacgaac ttgggtctcc acggaagcag ggtcaacgcc cacatgccat tgcggatcgc
     6781 cccacaacct taaccccctg caccaacccc caactgggcg tcaaaagcca gcacaaggga
     6841 cttcatcgat gtgcccttaa cggtatttta tccccgtctt cgagaaagcc aacaccgact
     6901 aggatactgc gccacctatc ctgttcgatc aacattatac cgcaaaacct agacccattc
     6961 aatggcagct gcgcgagcgt gtcggtcggg agctgtcagt catccatatg gctgacaatg
     7021 ccaggatcga cctggcttga acggctcaac acttccggca acgcaggttc caagccacag
     7081 agatttctcg acagacttct atattaaacg gatcgagcga agcgtgcatc gtccaaaaac
     7141 ggctagcctt tcaatgccca tcctatcatg agccaagaca ccacaatccc gaggaaggcg
     7201 cccattgcca cttcgagcgg tgtgtgtccg agaagctccc gtaacttcgt agactccaga
     7261 tacccctccc gaaacgccct ctcgataagc tggtttagga cttgcgcatg ctccccggca
     7321 gcccgcctga tacctgcggc atcgtacatc acgacaaggg caaatcccac cgtcaccgcg
     7381 aacagagctg agttccatcc ttcctggatc cccactccca ctgcgatact tgtaacgaag
     7441 gccgaatggg aactgggcat gcctcccggg cctacaatct ttgaccagtc gggcttgcct
     7501 tcagcccata tgcatgtaaa taccttgagt gtctgggcta tggcccaggc caatatggat
     7561 gtccacagta ttctgttgct aaacaatcct gtatcgggcg caaacatcag ctcaccacta
     7621 ccattccctg tcgacaatgt caattatgtc tgtcaactct gaagcagcgt ctcccaaaac
     7681 tgcacatttc gtccttgcag aatccattag agccgcaaat tgccgtcgcg caccatcgat
     7741 tccccatacc gagaccgcgg tcaggaatcc acttcttcca ctgtcccttt gagaatctgc
     7801 atcttcgagg tcgtcacgga tctgaaaagc aagcccaatg tcggcagcga t
//
LOCUS       DAOSAI010000067         7803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6029_length_7803_cov_4.036655, whole genome shotgun sequence.
ACCESSION   DAOSAI010000067 DAOSAI010000000
VERSION     DAOSAI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6029_length_7803_cov_4.036655"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLM83_03700"
     CDS             <1..405
                     /locus_tag="PLM83_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK70546.1"
                     /translation="TGSSGERTERLESLLEQFDLVGLRRQMGFTLSGGERRRVEIARA
                     LAAEPRYILLDEPFTGVDPIAVGELQRIVENLKEAGLGVLITDHNVRDTLAIVDRAYI
                     MHEGRTIVSGTAREVSEDAFARRFYLGERFTL"
     gene            434..1756
                     /locus_tag="PLM83_03705"
     CDS             434..1756
                     /locus_tag="PLM83_03705"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HOK70547.1"
                     /translation="MGGVVLIIVLTIAGGVIAYVGDRIGMKVGRKRLSIFGLRPKYTS
                     MVITIITGVIIAGCTLLVLSLASENVRIALFEIRELQDSLATANNDLEAKRAEVDVLS
                     ARVREVADEYDALESKFEAANRELDRVSHERSRALQELAKVESKLRDSESRLKETETR
                     YKQAGADLARASGELERARDEIASLESEKMSLERNVSSLSAAQASLEKSLEQMKLDMT
                     AQAATSALIMAEAEKSSWYGEVVFRAKEVILGEVVDCSRPRTEVNNQVTEILLKVNAI
                     ASKRGARSSRDDDPFVLLFDEGNYLAAIKDLHEAEGKMIFRVLSYKNSWYGDPLVVEV
                     EVFPDAKIYSAGDVIVSRTINGAAGGAEVQKELLALMEDVNAACIGKGMTSDEEGRIG
                     TIMTISDFTSAIVEIVKSNANVKVEAVARRDIWRSHKSPPVELVMQGI"
     gene            2045..>3339
                     /locus_tag="PLM83_03710"
     CDS             2045..>3339
                     /locus_tag="PLM83_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOK70548.1"
                     /translation="MKKLLAVLVATALIGIIAIPVLASPFSDLPEESHWALDALTMLA
                     ALGIVEGYPDGSFKGPQPATRYEVALIVARALEYLDRDIRQMSEKMFDLEGRLDAGVV
                     QQEAKPEAEPVLQPSASPDATILEQVIYEKIAAMSDEQWVNFEEKLYELVARVDDLSE
                     DNKAEHAKLWEAVEALEALKEARTTTVVVSEGADIGLALDSQKAEIMKALSDAVNAEK
                     DERQKAIDVLGASMDAALSEQKGEIMMALYDSLEAAKDEGSREMAELEAKLQLALDEQ
                     NAALTMAFYDSLKAAAEGDEQALQGLSGAWQLALDEYNAELTMAFYDSLKASKEETDR
                     AIEDVLAKLDVALAEQNAELTMAFYDSIEAAKDEQDRKMAAMEAEWQLALDEQNAELM
                     MAFYDSIRAAQNDNAADLEALKAEWQLALDEQNAELMMA"
     assembly_gap    3340..3439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3440..5625
                     /locus_tag="PLM83_03715"
     CDS             <3440..5625
                     /locus_tag="PLM83_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70549.1"
                     /translation="AEWQLALDEQNAELMMAFYDSIQAQREDIMAMFEDTESRFELAL
                     DELRAELMMAFYDSVEAEADERARAIDELAEDFDLKLMELHAEVAMETYDSVFAAREE
                     CLAAVDAVVATIDALQAEFSRELAALNLRVDAVERELAVVRGMADQNAKDIAETYHRI
                     KTEDIAPLEERVAKLEGKAAEHDEKISGIWDDLNRFRFTGTNTAQFTDIDLRGEGFYQ
                     DPWNPAGKTYAPTSVLQNKLELELNIVPEKGVSIDVGLSAVTDIFGKDDSEVGLFDGG
                     LNLDVTTPSGNAKVLAGDLARPRNFTKYQISRKVFGDDGVEGLSLDLTTGPFVTTGMI
                     AKIEPAEYEMQYVLGGGASYTLGSLKVDGRLLKITDDPESGLEAVTPNESVAGTGFAL
                     KLGEGWKLDGEISSWNKDNEATGLLARNLHAAGKAGVLNLTGYFEQVDDGYAPKYLGT
                     ASNDPDRVVADTRKAGFEVKTDSLNGLTITGSIDRAGDAWYASKDVVTYATDAEYEME
                     LGPIDVTLRGGLAQDHNGYPTVNNPRFATTTDLGFDVAFSPLKAGFTWEHEVEANATS
                     YIGYADLNMPLAADTLALKGNWKQSFGSNEYYTYGLGIDMKMPITENVSTMGGTMSYG
                     KSSGMARDKDNYARFLVGADLEWKLRPATTFTGKASYELRDYEKDTQPSGEYLQYGLA
                     LSHEIYKSTTLKMNYDFKDVYYSPRNNELTNYSARIIGLSLKTVF"
     gene            5692..6093
                     /gene="ruvX"
                     /locus_tag="PLM83_03720"
     CDS             5692..6093
                     /gene="ruvX"
                     /locus_tag="PLM83_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOK70550.1"
                     /translation="MDEIVLAVDPGREKAGIAVVSRERGTMHKCVIEASECVEQVCEL
                     AEKHGVARIVIGDRTGSSEIIEALGHVCDLEIIAVDEHMSSMEARERYLRENPGKGIA
                     RFLPIGLRTPKEPYDDYVAEILAARYFDSVS"
     gene            6176..7627
                     /locus_tag="PLM83_03725"
     CDS             6176..7627
                     /locus_tag="PLM83_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70551.1"
                     /translation="MSNHCRAGKIQCSACFILALSVFILAFSAHTIASSPLVPSEDWR
                     YRALAELGRGGLLYEDDAARYASGIPVTEKELSASVERTIYRLNQANGDSTSVVGATA
                     GPLKLSGGPSDTLRLSARQICLVADLVAEFGSSFSDPSLTRLPGSLPLDYEDGLALTL
                     RHATHGNAELHDILKLTANTGSVGYPIRTIEAVTTLDSLEAMLKQAVAHQEPQAQAKM
                     EMGVNSPIGMLAIGLSSEAAQRSTVPARDYSEPATAQGFDLSTSVQLSDILRFTASFA
                     TDDKAVERPTSTAVGVSIGEQEGSGVSVGHRVTDLAGPAPNSLRETITSVDVKYSLPD
                     AGGKASGPDSLTVRAGYELYGREPITLDGEKNSLQATTSLAIDYRLLFGEAASLQAAY
                     RYEHVRDLVASGAVWARDGLYETSLYGWPSEGSLKLVEGSDAVTRTVASIDFSYRLMN
                     DASVMLGYKLIDFTEINALDPKNMATAEVTIRF"
BASE COUNT         1837 a         1783 c         2366 g         1717 t
ORIGIN      
        1 acggggagtt ccggtgagcg gacagagcgc ctggaaagtc ttcttgagca gttcgattta
       61 gtcggtttgc gtcgacagat ggggttcaca ttgtcaggcg gagagcgcag gcgagtggag
      121 atagcgcgcg cgcttgcggc ggagcctcgg tacatattac tggacgaacc cttcactgga
      181 gtcgatccaa tagccgtagg agagttgcag cgcatagtgg agaatctgaa ggaagcaggc
      241 cttggggtgc tcattactga ccacaatgta agagatactt tggccattgt ggatcgggcc
      301 tacatcatgc atgaaggccg cacgattgtg tcagggaccg cgcgggaggt ttcggaggac
      361 gcttttgcaa ggcgtttcta cttaggagag cggttcaccc tctaagcctt gttcttgccg
      421 ggagggatac gtgatgggcg gagtcgtact gataatcgtc ctaacaatag ccggaggcgt
      481 gattgcctat gttggcgatc gtatcggcat gaaggtgggc cgaaaacgcc tttcaatctt
      541 tgggctgagg cccaagtaca cttctatggt gattaccatc atcactggcg tgatcattgc
      601 cggatgcact cttttggttc tttctctggc ttctgagaat gtaagaatcg ccctcttcga
      661 aatccgtgaa ctccaggatt cgctcgctac tgccaacaat gacctcgaag ccaagagggc
      721 tgaggttgat gtcttatccg ctcgagttag ggaggtagcc gacgaatacg atgctctgga
      781 atccaagttt gaagcggcca atcgagaact ggatcgagtg tcgcatgaac ggtcgcgggc
      841 tttgcaggag cttgccaaag tagagtccaa actgcgtgat tccgaaagcc gactgaaaga
      901 gaccgagact agatacaagc aagcaggcgc agatcttgct cgcgccagcg gcgagcttga
      961 aagggcgcgc gatgaaatag cgtcgctcga aagcgaaaag atgtcgctgg agcggaacgt
     1021 atcttctctg tctgcggcgc aggccagcct cgaaaagagc ctggagcaga tgaagctcga
     1081 catgacagct caggcggcca caagcgccct cataatggcg gaagcggaga aatcgtcgtg
     1141 gtacggagag gttgttttcc gagccaaaga ggtgattctc ggcgaagtgg tggattgctc
     1201 gcggccaagg acagaggtga acaaccaggt aacggagatt ctgctaaaag tgaatgcgat
     1261 agcttccaag aggggagccc gttccagcag ggatgatgat ccatttgtcc ttctgttcga
     1321 tgaaggcaac taccttgctg ccataaagga cttgcatgag gctgaaggaa agatgatctt
     1381 tagggtgctg tcgtacaaga attcctggta tggagatccg ctcgttgtag aagtggaggt
     1441 atttcccgat gccaagatct actcagcagg ggatgttatc gtctccagga cgattaatgg
     1501 tgccgccggc ggagcggagg tccaaaaaga gcttttggcg ctcatggaag acgtcaacgc
     1561 agcctgcatc ggaaagggaa tgaccagcga tgaggaaggc cggatcggga ccatcatgac
     1621 aatctccgat tttacttctg cgatagttga gattgtaaag agcaatgcca atgtaaaagt
     1681 ggaggccgtg gcgcgtcgag acatatggcg gtcgcacaag tctcccccag ttgaactggt
     1741 gatgcagggc atttagcctg tgggcggctg gtggagtatt caaaaatctt ctgattcatg
     1801 aaggagttga ccagtgtttg gagaatatga aattgcaggt atactcttgg tcatacctat
     1861 gttctgatcg gccctaacaa cggcaggaga aagctgtggt tgtttggccg tattgtacgc
     1921 aaaagggctc gtccccgttt tggttgcagg gcgaggaaac atgagcactc gtcctggtca
     1981 cctctatcgg cattgagttc ttagcgtcta gttgatcagg atcaatttga ggggggaagg
     2041 actaatgaag aaactcctgg ccgttctcgt tgccaccgcg ctgattggca tcattgccat
     2101 cccggtgttg gcgagtccct tctccgattt gccggaggag tcgcactggg cacttgacgc
     2161 actgacgatg ctagcagcgt tggggatcgt cgagggctac cctgatggat cattcaaggg
     2221 accacagcct gcgacaaggt atgaagtggc cttgatagtg gctagggcac tggaatacct
     2281 cgacagagac atcaggcaga tgtccgagaa aatgtttgac cttgaaggca gattggatgc
     2341 tggggttgtt cagcaggaag cgaaacctga agcggaacct gtcttgcagc cttccgcgtc
     2401 gcccgatgca accatcctag agcaggtgat ctatgagaag atcgctgcta tgtctgatga
     2461 gcagtgggtg aattttgagg agaagcttta tgagctagtt gcgcgggttg atgacctcag
     2521 cgaggataac aaggctgaac atgctaagct ttgggaagct gttgaggctc ttgaggctct
     2581 taaagaggcg cgcactacca cagtggttgt gagcgagggt gccgatatcg gattggcttt
     2641 ggatagtcag aaagccgaaa ttatgaaggc gcttagcgat gctgtcaatg cggagaaaga
     2701 cgaacgccag aaggcgattg atgtcctagg cgcgtcgatg gatgccgccc tatccgagca
     2761 gaagggcgag atcatgatgg cgttgtatga ttcgctcgaa gctgctaagg atgaaggaag
     2821 tcgtgagatg gcagagctgg aggccaaact ccaactagct cttgatgagc agaatgccgc
     2881 tttgactatg gccttctatg attcccttaa agctgcggct gaaggcgatg aacaggcgct
     2941 tcagggtctg agcggtgctt ggcagctggc tctggatgaa tataacgccg agcttactat
     3001 ggccttctat gattctttga aggcttccaa agaagaaact gatcgcgcca tagaggatgt
     3061 cctggcaaag ctggacgtgg cgctcgcgga gcaaaacgcc gagctcacca tggctttcta
     3121 tgattccata gaggccgcca aagatgagca agaccgcaaa atggctgcta tggaggccga
     3181 gtggcagttg gctctcgacg agcagaacgc cgagctcatg atggccttct atgattcgat
     3241 ccgcgctgca cagaatgaca acgcggctga tctcgaggcc ttgaaggccg agtggcagtt
     3301 ggctctcgac gagcagaacg ccgagctcat gatggccttn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnna ggccgagtgg cagttggctc tcgacgagca gaacgccgag
     3481 ctcatgatgg ccttctatga ttccatccaa gctcaacgag aagacataat ggccatgttt
     3541 gaggatacgg agtccagatt tgagctcgcg ctcgatgaac tcagggccga gctgatgatg
     3601 gctttctatg attcggttga agctgaagcc gatgagagag caagagccat tgatgagttg
     3661 gcagaggatt tcgatctcaa gctcatggag ctccatgccg aagtcgccat ggagacctat
     3721 gattcggtct ttgcggctcg agaagagtgt ctggcagcgg tcgacgccgt tgtggcaacc
     3781 atcgatgctc tgcaggctga gttcagccgg gaactggctg ctcttaacct tcgcgtagac
     3841 gcggtggagc gggaattggc tgtagtccgc ggcatggctg accagaacgc gaaggatatt
     3901 gccgaaacat atcacaggat aaagactgaa gacatagctc cgctcgagga gcgcgtcgcc
     3961 aagcttgaag gcaaggcggc agagcatgat gagaagatct cagggatctg ggatgacctg
     4021 aacaggttca ggtttactgg aaccaacacg gcgcagttca cagatattga tctgcgcggc
     4081 gaagggttct atcaggatcc ctggaacccg gctggcaaga cgtatgctcc tacatcagtt
     4141 ctccagaaca agctcgaact cgagctgaac atagttccgg aaaagggcgt aagcattgat
     4201 gtagggctca gcgctgtaac tgacattttt ggcaaagatg acagtgaggt tggcctcttc
     4261 gatgggggtc tcaatctcga tgtgaccacg ccgagcggca atgccaaggt gctcgcaggc
     4321 gatttggcca gaccgaggaa cttcactaag tatcagatca gccggaaggt atttggggat
     4381 gatggtgtag agggtctcag cctcgatctg accaccgggc cttttgtcac tacaggaatg
     4441 attgcgaaga tcgaacctgc tgagtatgag atgcagtatg tgctgggcgg cggagccagc
     4501 tacacattgg gaagcctcaa ggtggatggc aggctgctca agataactga tgatccggag
     4561 tcggggctcg aagccgttac gcccaacgag agtgttgctg gcacagggtt tgcgctgaag
     4621 ctcggcgaag gctggaagct tgacggagag atctcttctt ggaacaagga taacgaggcc
     4681 acgggcctct tggctcgcaa cctgcatgca gcaggcaagg ctggggtttt gaacctcaca
     4741 ggctacttcg aacaggttga cgatggctat gctccgaagt atttgggcac ggccagcaac
     4801 gatcctgaca gagtggtcgc cgacacgcgc aaagcaggct tcgaagttaa gactgactcg
     4861 ctcaacggtc tcaccatcac cggcagtatc gatcgcgcag gtgatgcgtg gtacgccagc
     4921 aaggatgtag tgacttacgc aaccgatgcc gagtatgaaa tggagcttgg acctatcgac
     4981 gtcaccctca ggggcgggtt ggctcaggac cataatggct atcccacggt gaataacccc
     5041 aggttcgcta ctacgactga tcttgggttc gatgtggcat tcagccctct gaaggcaggg
     5101 ttcacctggg aacatgaagt ggaagccaat gcaacttcgt acattggcta tgcggacttg
     5161 aacatgccgc ttgcagctga tacgttggcc ctcaagggta actggaagca gagcttcggc
     5221 agcaatgagt actacaccta tggcttgggc attgatatga agatgccgat caccgagaat
     5281 gtctcgacaa tgggcggaac catgtcttat gggaagagca gtggcatggc cagggataag
     5341 gataactacg cgagattcct ggtgggcgca gacctcgaat ggaaactgcg gccggcgact
     5401 acctttacag ggaaagcctc ctacgagctg cgcgactacg aaaaggatac gcagccttca
     5461 ggcgagtatc tacagtacgg gttggcgctc tcgcatgaga tctacaagtc cactacgctc
     5521 aagatgaact acgacttcaa ggacgtctac tattcgccaa ggaacaacga attgacgaat
     5581 tactccgcga ggatcatagg cctatccttg aagacggtat tctaagaata gccgttggat
     5641 gccccgggga gcgtatgtcc cccggggcat tttcatagga ggtgatgtgc tgtggacgag
     5701 atagtgcttg ctgtagaccc agggcgggag aaagcaggaa ttgcagtagt atcacgggaa
     5761 cgtgggacga tgcataaatg cgtgatagag gcgtcggaat gtgtggagca agtgtgcgag
     5821 ttggccgaaa aacatggcgt tgcgaggatc gtgattgggg acaggactgg atcgagtgaa
     5881 ataatagagg cgttaggaca tgtttgcgac ctggaaatta tcgccgtcga cgagcacatg
     5941 tcctctatgg aggcgcggga acgctatctt cgagagaatc ctggaaaggg aatcgctcgc
     6001 tttttgccga tcgggctgag gaccccgaaa gaaccttatg acgattatgt agccgagatc
     6061 cttgcggctc gatattttga cagtgtatca taggagagga atcgtaggat ttgtgaagaa
     6121 tatagtacaa actgcggagg atagcgcagt gtagagcaga gggggcgaaa gaagtatgag
     6181 caatcattgt cgggctggga agatacagtg ctctgcctgt ttcatccttg ctttatccgt
     6241 cttcattctt gcgttttctg ctcatacgat cgcctcgtcg cccctggttc cttctgagga
     6301 ctggagatac agagcccttg cggaactggg gcgcgggggg cttctgtacg aagatgacgc
     6361 tgcccgatat gcgagcggta ttcccgtcac cgaaaaagag ctgtctgcca gtgttgaaag
     6421 gaccatttac cggctgaacc aggctaatgg cgattccaca tcggtagtgg gcgctacagc
     6481 tgggcctttg aaactttcag gcggcccgtc ggatacgctg aggctatcag ccaggcagat
     6541 ctgtcttgtt gcagatctcg tggcggaatt cggatcgagc ttctcggacc cgtccctgac
     6601 gaggcttcca ggaagccttc cactagacta cgaggacggg ctggcgctca cgcttcgcca
     6661 cgctacgcac ggaaatgctg agctgcatga tattctcaag ctaacggcga acacaggcag
     6721 tgttggctat cctatcagaa ccatagaagc ggtcacaacg ttggatagcc tggaggctat
     6781 gcttaagcag gcggttgcac atcaggagcc tcaggctcaa gccaagatgg agatgggagt
     6841 caacagcccg attggaatgt tggcgatcgg gctgtcgagt gaggccgctc aacgttcgac
     6901 tgtgccggcc cgggactact ccgagcccgc gactgctcag ggttttgatt tgtctaccag
     6961 cgtgcagtta tccgatatcc ttcgattcac cgcaagcttt gcgacggacg acaaggctgt
     7021 tgagaggcct acatccacag cggtaggagt ctccatagga gagcaggagg ggtccggggt
     7081 gagcgttggt catagggtaa cggatcttgc cggccctgcc ccgaatagtt tgcgcgagac
     7141 tatcacatcg gtagatgtga agtattcgct tcccgatgcc ggcgggaagg cttcagggcc
     7201 tgattctttg accgtgaggg ctggctacga gctctatggc agagagccta tcacgcttga
     7261 tggtgagaag aatagcttgc aggcgactac ttctttggcc atcgattaca ggctgctttt
     7321 cggggaggct gcatcactac aggcagcgta caggtacgaa catgttcgcg accttgttgc
     7381 atccggagca gtgtgggcga gagatggcct gtacgagacg agtctgtatg gttggcctag
     7441 tgaaggctca ttgaagcttg ttgagggttc tgacgccgta actcgcactg tggcgtcgat
     7501 cgacttcagc tataggttga tgaatgacgc ttcggttatg ttaggctaca agctgatcga
     7561 ttttactgag ataaatgccc tcgatcccaa gaacatggcg acggccgaag tcaccatcag
     7621 attctaaaga gtctttcatc gtaccagtaa ggggaggtag ctcaatgaga atgtcgaagg
     7681 ctcaagtcgt aaccatgatt gtattatgtg tggtgctatg tcttggcggg ggttatgccg
     7741 taggcaattc aagtcctgtg gagcgcctta tcggcatgga acggctccta tacggcgggc
     7801 ctc
//
LOCUS       DAOSAI010000068         7797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6034_length_7797_cov_4.416430, whole genome shotgun sequence.
ACCESSION   DAOSAI010000068 DAOSAI010000000
VERSION     DAOSAI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6034_length_7797_cov_4.416430"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="PLM83_03730"
     CDS             <1..1472
                     /locus_tag="PLM83_03730"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOK70552.1"
                     /translation="PSERREIERQLFSGELLGVVSTNALELGIDIGSLDASIIVGYPG
                     TIASTWQQAGRAGRKGEESLSVFIAHDLPIDQYLMGHPEYFFDRSPEHAVVNPANPYI
                     LVGHLRAAAMELPISPLELADFGEYAPALIELLEEKGEVLRTRLGWRWIGRGFPANDV
                     KLRNMSENTYTIVDTSSDHGNRVIGTIDELSAFEQVHPEAVYMHEGETYLVSDLNLVE
                     KVAYIHKADVDYFTQSVTETRVQIEEKEQNKMWRKSEVEFGDVTVTNLTYMFRKIKFY
                     ERDSIGYGKVNLPENNLPTTAAWIMLPESAAKLVASLGRIPAEGLIGIGNAASAVIPL
                     FAMCDSMDIGTAVDSSNTGVPTLFIYDRHQGGVGFAQKAYKMIDEVMEACLNLIQNCP
                     CEDGCPSCVGSPIPPYAQNDPDATSRGRIPDKDTALIILHNLLEKEPYVPAGPPKWAE
                     GSDNADGACAGYSEEDFRIDVKPLPDGVERRIRKMINSR"
     gene            1906..2649
                     /locus_tag="PLM83_03735"
     CDS             1906..2649
                     /locus_tag="PLM83_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOK70553.1"
                     /translation="MKRLLALACLCSLVVVAFAAPGVGAAKPVLKVGTSADFPPFEFQ
                     DEKTGAYLGFDLDLIRAIGDAIGMDVQIVNTAWDGLIPGLITGNYDCIVSAMTITDER
                     LKAVNFSDPYFSAGQVVVTMAKDTKISSIADLEGKKISVQIGTTGDLVASEIKGATVK
                     RFNLAPDAVQEVRNGAAHACIIDLAVAAEIAKEYKDIKYGAPFTMEYYGIAIKKNNTA
                     LLKNINRGLAMIKASGKYDEIYNKWFAGN"
     gene            2833..3468
                     /locus_tag="PLM83_03740"
     CDS             2833..3468
                     /locus_tag="PLM83_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOK70554.1"
                     /translation="MPALLAGAKMTIELAVISVAIGFVIGSLVGIARVSQNRALRVIA
                     GIYVDFIRGTPLLVQLFLVYFGLPTLIGRPVPPFIAAISAMGINSGAYVAEIVRAGIQ
                     SIDRGQTEAARSLGLTSSQTMQYIIFPQAFRRIIPPLGNEFIAMLKDSSLVSVIALED
                     LLRKGQVVITRTFRPFEVYTVVALIYLVMTFVISKLVSWSERKLKAVGQID"
     gene            3461..4183
                     /locus_tag="PLM83_03745"
     CDS             3461..4183
                     /locus_tag="PLM83_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOK70555.1"
                     /translation="MIKTVGLRKSFGHNEVLHGIDATINPHEVVVVVGPSGSGKSTFL
                     RCLNLLEKPTAGEIYFDGQLITAPGVNVNEIRREMGMVFQSFNLFPHLTVLDNIALAP
                     KIVRHVSKDEAEETARDLLRKVGIADKESAYPQELSGGQQQRVAIARALAMKPMAMLF
                     DEPTSALDPEMIGEVLDVMKALAVEGMTMVVVSHEMGFAREVADRVLFMDAGLIVEEG
                     PPAQVLGDPKNPRTKAFLGKVL"
     gene            5033..5794
                     /locus_tag="PLM83_03750"
     CDS             5033..5794
                     /locus_tag="PLM83_03750"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOK70556.1"
                     /translation="MKGRHFGSRLSLLAVICAFCLTTSGCNMGDYTFVDLPVRVAWEP
                     DQLIRVIDSEKVFGHRLGRGDGLRVWLDLGPLPCDLEPESIERATLKLPCQSFSRVAG
                     AVTFEIYMVKWTYDSGMGEWPDIPEFLHSSELPNDVQPISSARLSHDVGWETQHSNGY
                     VYSSQYIDTEVKYLSFDITEAIKAWVSGTPNAGLRIKQKSPARPDGPVWSLLMAPDKR
                     LKDIHKALDELPKSANGPSDQPIAIVCIKKAGEAS"
     gene            5903..6667
                     /locus_tag="PLM83_03755"
     CDS             5903..6667
                     /locus_tag="PLM83_03755"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOK70557.1"
                     /translation="MEQAKRVFVRTKTADRASGATERASSEGHKRRWKCDWAAPRNRL
                     KSCPRGMRLSQRPDSGKGVILTLARIGAWFVLIGLILGVVSGGLFVGPFGGYIKKPPR
                     APLDSEHGDGLLPVKPSPGYRAPNFVGRLPDGTIISLDDCAGMPIILVFFRITSAPSI
                     AQMPAVDRLYRERQDRCCVIAVAVFEREDDVGRYMAANDLDIPMIADIRGEIAQAYDI
                     TALPTTFIIDGQRIIKDLRIGAMTYAELERALKPLY"
     gene            complement(6651..7535)
                     /locus_tag="PLM83_03760"
     CDS             complement(6651..7535)
                     /locus_tag="PLM83_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOK70558.1"
                     /translation="MTGSLVFEFFRRIATPGLDRVVKIITDLGSISFYMIAIPIIYWC
                     IDKSVGFNLGMVTSISSCINVGLKFVFKVPRPYVAYPHLDAPNFLLDTGTGWSFPSGH
                     AQGTSTFWAYSAWHVKRAWFTALSVIVVAVVGLTRIYATVHYPKDVLVGTALGLIIVQ
                     VYMLVEESLANREKKTSVNTAVAASIVIPGVIFALSSLSFELARESASLLGFTMGLGV
                     GYALEKEYVKYDVRASFFIQVIKVVFGLAVLMGIRYGLKAILPEGSFWQMLRYAVMAL
                     WGTYGAPLVFKAMFSKEA"
BASE COUNT         1929 a         1877 c         2205 g         1786 t
ORIGIN      
        1 ttcctagtga acgaagggag atcgaaaggc agcttttctc gggagaactg ttgggagtgg
       61 tgagcactaa tgctctggaa cttgggatcg atatcggttc cctcgacgcg agcatcatag
      121 taggctatcc cggaacgata gcgtccacat ggcagcaggc tgggcgcgct gggcgaaagg
      181 gcgaagaatc actctcggta ttcatcgccc acgatctgcc tatcgaccaa taccttatgg
      241 ggcacccgga atactttttc gaccgttctc ccgagcatgc agtcgttaat cccgcaaacc
      301 cctacattct cgtgggccat cttcgcgccg cggcgatgga actccctatc agtccgctgg
      361 aacttgcgga ttttggagag tatgcgccag ccctcataga gctgcttgaa gagaagggag
      421 aggttctcag gactagactg ggctggcgtt ggattggccg gggctttccg gccaatgatg
      481 tgaagctccg taatatgtcg gagaatactt acaccatagt ggatacctca tctgaccatg
      541 ggaaccgggt tatcggcacc atcgacgagc tgagcgcatt cgaacaagtc cacccggagg
      601 ccgtgtacat gcatgaaggt gaaacatatc tagtgtcaga cctcaatctt gtggagaagg
      661 tggcctacat ccacaaggcg gatgtggatt atttcaccca atcggtgacg gagacgcgcg
      721 tccagatcga ggagaaagag cagaataaga tgtggaggaa gagcgaagtc gagttcggtg
      781 acgtaacggt caccaatctg acatatatgt ttcggaagat caagttctac gagcgcgaca
      841 gtatagggta tggcaaggtc aacttgccag agaacaacct tcccaccact gcggcatgga
      901 tcatgctccc tgaaagtgcc gcgaagctcg tggcgagcct cggcagaatt ccggcggagg
      961 gcctaatagg aatagggaat gctgcatctg ctgttatccc tctatttgca atgtgcgatt
     1021 ccatggacat aggcacggcg gtggattcct cgaacacggg cgtgcccacg ttgttcatat
     1081 acgataggca ccagggtgga gtggggtttg cgcagaaggc ctacaagatg attgacgagg
     1141 ttatggaagc ctgcctaaac ctgatacaga attgcccgtg tgaagatgga tgtccttcgt
     1201 gtgtaggttc acccatacca ccgtatgccc aaaacgatcc ggatgcaaca tcgagagggc
     1261 gcattcccga caaagatacg gccctcatca tcctccataa cttgctcgag aaagagccgt
     1321 acgtcccggc aggtccgcca aaatgggccg aaggctccga taacgcggat ggcgcctgcg
     1381 cgggctacag cgaggaagac ttcagaatag atgttaagcc cttgcccgac ggtgtcgagc
     1441 ggcggatcag gaagatgatc aacagcagat gacgcccgag gttggccttg aaggctcgat
     1501 catgtagttg gtttgccatg atatcttagg cgacgaggat gagccggttg aatcgggcaa
     1561 ctcgaatatg ttgcacgcgt tgtgcggata acggacacaa gatctgacga attcatgaaa
     1621 cgacactccc catgcccatc tcctgccaca attgatccga aagagcgatg cttatgcaag
     1681 acccgaatat tataaatata cctcaaataa ctacgaaaaa ctgaaggcgg atgctgcatc
     1741 gatcggctga tgtgaaaacg agataaccca catatacgtg gggatattac gctcattacg
     1801 aatcgggcgc cctgaaattc tgacgcgtgc tattgacaat aggcctccga gcagtgtata
     1861 ctcatgcagc agatacgaat tatggtggag gagaggtcag ctgtcatgaa aagactgtta
     1921 gcccttgcat gtttatgttc gttggttgtg gtggcatttg cagcacctgg tgttggagcg
     1981 gccaagccag ttctcaaggt gggaactagc gccgacttcc cgccttttga gtttcaggac
     2041 gagaagaccg gggcatatct gggattcgat ctcgatctga tcagggcgat tggggacgcg
     2101 atcggaatgg atgttcagat tgtgaacact gcttgggacg gcttgatacc tggacttatc
     2161 actgggaatt acgattgcat tgtctcagca atgaccatta ctgacgagcg tctcaaggcc
     2221 gtcaactttt cggacccgta tttcagcgcc gggcaggttg tagtcaccat ggccaaggat
     2281 accaagatat cgtcaattgc cgaccttgag ggcaagaaga tatcggttca gataggaacc
     2341 actggggatt tggtggcatc agagattaag ggggcaacag tgaagcggtt caatctggct
     2401 cctgatgcag ttcaggaagt gaggaatggc gcggcccatg cctgcataat cgatttggca
     2461 gtggctgctg agattgccaa ggaatacaaa gacataaagt atggcgcgcc tttcacaatg
     2521 gaatactacg gaatcgccat caagaaaaac aataccgctt tgcttaaaaa cattaatcgg
     2581 ggattggcaa tgatcaaggc ttccggaaag tacgacgaga tatacaacaa gtggtttgcc
     2641 ggcaactagt ggcaggctaa tgccttgcgt ggaagatatt gaggccgtgc gcgcttggag
     2701 agatgacctc tccagcagct cacggcccat tttaggcgct tgggacttca tttggttctc
     2761 aagcgataga ttagaagatg acactttggg ggagttgccg tggctctcga tttttctata
     2821 ataccaggta tcatgcctgc cttgctggcc ggagccaaaa tgaccataga gcttgcagtg
     2881 atatcagttg cgatcggatt tgttataggc tcattagttg gaatagcgcg ggtcagtcag
     2941 aacagggcgc tcagggttat tgcaggcata tacgtagact tcattcgagg gacgcctctc
     3001 ttggtccagc tgttcctcgt ctattttgga ttgccgacac tcataggtcg gcccgtgccc
     3061 cccttcatag ctgcgatatc ggccatgggc ataaacagcg gtgcatacgt ggctgagata
     3121 gtgagggcag gaatacagtc gatagacaga gggcagacag aggcggccag atctttagga
     3181 ctgaccagtt cgcagacgat gcaatacatc attttcccac aagcgtttcg acggataatc
     3241 cccccattgg ggaacgaatt cattgcaatg ctgaaggact cttcgcttgt atccgtgatt
     3301 gcactagaag atcttcttcg aaaagggcag gttgtcatta ccagaacatt caggccgttc
     3361 gaggtttata ccgtagtggc tctcatatac ctcgtcatga catttgtcat ttccaagctt
     3421 gtcagctggt cagaaagaaa actgaaggcg gttgggcaaa ttgattaaaa ctgtcgggct
     3481 cagaaaatca ttcggacata acgaggtgct gcatgggatt gacgcaacaa ttaaccccca
     3541 cgaagtggtg gttgttgtgg ggccgtcagg ttccggcaag agcacctttc tgcgttgtct
     3601 caacctgttg gaaaagccga ccgcagggga gatatatttc gacggccagc tcataaccgc
     3661 acccggagtg aatgtcaacg agattcgccg ggagatgggg atggtgttcc aaagctttaa
     3721 cctcttcccg cacctcaccg tgcttgacaa catcgcgctt gcccctaaaa tagtgaggca
     3781 tgtctcgaaa gacgaggcgg aagagacagc tcgcgatctg cttcgcaagg tggggatcgc
     3841 cgacaaggag tctgcctatc cgcaggagct gtcaggcgga cagcagcagc gcgtggctat
     3901 tgcccgcgcc cttgctatga aacccatggc tatgctcttt gacgagccca cgtctgcgct
     3961 agacccagag atgataggag aggtcctcga tgttatgaaa gccctcgccg tagaaggtat
     4021 gaccatggtt gtggtcagcc atgaaatggg ttttgctcga gaagtggcgg acagggttct
     4081 tttcatggat gccgggttga tcgtggaaga aggaccaccc gctcaggtgc tgggcgatcc
     4141 gaagaatccg aggaccaaag cgttcctggg aaaagtattg taggagtgct ttgctctttc
     4201 gggggtttgt gaggtacggg ccatgccatt ttttctccta cgacaggggt aaacgcagga
     4261 ggaaaagagg gcatggccca ttgaatttcc actgctttgc gactgcaggc tgcaatccaa
     4321 ccgatcgctt ggcacttggg tccgatatgg atccaagccg gggccatgcg ttcagccacg
     4381 gccccggtaa cggcggtgaa ttatctgcct gccgatgttg ggccgaaggc cattgtcaga
     4441 gcttcagtga aattgccgtg taggttcttt tccaaagtcc ccggaactgc cagatgtagc
     4501 ctggtgcttc ttccttcccc atccctggcc gggtgcattc caacgcagac cccatcttta
     4561 gctgcagcct ttcacgtgat gcaaggataa aaacgtggta cacgggcaca tatctcccta
     4621 tccggatttc gatggagcac atcgatactt gagattgaca ttactcaagc cgtgaaggcc
     4681 ttaatgtcgg gggcatcctc gaagagtgtg gcgacagcga ctgccaaggt ctgcatagtc
     4741 aaagcgggaa gggggcgccc ctagtgttgc agggtcagct gtgccgggaa gtcgctgatg
     4801 gttgtgttgg gctcatagtg agacaggact actgatgtgc atgccttgtg gggcaggtgt
     4861 tgtatgctct ttctgctttc gtgtggccca acatgaagga cgggccacgc catccttcag
     4921 tgaaatatga ggcatctatg gcaggataaa tagtatggta tgtcaaagca ctgggaaagt
     4981 gatacaatcc ggacactgta ggtattgacc aatgctacag ggaggttatt gaatgaaggg
     5041 aaggcatttc ggttcgcgcc tcagcttgct agctgttatt tgcgcgtttt gcctgacgac
     5101 gagcgggtgc aatatgggag attacacctt cgtagacctt cctgtcaggg ttgcttggga
     5161 gccagatcag cttatcagag tcatcgactc ggaaaaagta ttcggtcaca ggttggggag
     5221 gggcgacggg ctgagagtgt ggctcgacct tggtcctctg ccctgtgatc ttgagccaga
     5281 gtccattgaa agagccacat tgaagttgcc ctgccaatcc ttttcgagag tggcaggtgc
     5341 cgtcacattt gagatataca tggtcaaatg gacgtatgat tcgggaatgg gcgaatggcc
     5401 cgacattcct gagttcttac actccagcga acttcctaac gatgtacaac ccatctccag
     5461 cgccaggctt tctcatgacg tagggtggga gacgcaacat tcgaacggat atgtctatag
     5521 ctcccaatac atagatactg aagtcaagta tctcagtttc gatattaccg aagcgataaa
     5581 ggcgtgggtt tccggcactc ccaatgcggg ccttaggatt aagcagaagt ctccggcgcg
     5641 cccggatgga cctgtatgga gtctcttgat ggccccggat aaacgtttga aggatattca
     5701 taaggcatta gatgagcttc cgaagagtgc gaatggcccg tctgaccagc cgatagcaat
     5761 agtgtgcata aagaaagccg gagaagcttc ctgaatcaca gagcacctag ttatgtccga
     5821 tataatgctg taaaaaaggc cagagacagt tgtaatagtg gcctagcctc cgttcgtaca
     5881 cattttagcg gagggatctg caatggaaca agcgaaacgg gttttcgtga ggactaagac
     5941 tgccgacagg gcttcgggtg ctaccgaacg ggcgagctcg gaagggcaca agcggagatg
     6001 gaagtgcgat tgggctgcgc ctcgaaatag gctgaaaagt tgtccccgag gcatgaggct
     6061 ttcccagcgc cctgatagcg gaaagggagt catcctcaca ttggccagaa taggcgcttg
     6121 gtttgtgctg atcgggttga tacttggagt tgtatcaggc gggctatttg tggggccctt
     6181 cggaggctat atcaagaagc caccgcgtgc gcccctcgat tcggagcacg gcgatggctt
     6241 actccctgta aagccctccc cgggttatcg tgcgcctaat ttcgtaggca ggctgcctga
     6301 tggaactata atatccctcg acgattgcgc aggcatgccc ataatactag tcttcttcag
     6361 aatcacgtct gccccgtcca ttgcccagat gccggctgtg gaccgacttt accgggagcg
     6421 gcaggaccga tgttgcgtca tagcagtcgc cgtcttcgaa agggaggatg acgtaggccg
     6481 ctacatggca gcaaacgatc tcgacattcc catgatcgcc gatatccgag gcgaaatagc
     6541 tcaagcctac gacatcaccg cgcttccgac cactttcatc atagatggcc agaggataat
     6601 aaaggatctc agaataggcg caatgaccta tgcggagttg gaacgagccc tcaagcctct
     6661 ttactgaaca tggctttgaa cactaggggt gctccatatg tgccccacag cgccatcaca
     6721 gcgtagcgca gcatctgcca gaaagaaccc tcgggtaata tcgctttgag accgtaacgt
     6781 atccccatta gaactgccag gccaaatacg actttgatca cttgaatgaa aaaacttgcc
     6841 cttacatcgt acttcacata ttccttttcg agagcgtatc caactccgag gcccatggtg
     6901 aatccaagaa ggcttgcgga ctcgcgggcc agttcgaagc tgagggatga gagggcgaag
     6961 atcacgcctg gaattactat agacgcggcg acagcggtgt tgacagacgt cttcttctcc
     7021 cgattggcca aggactcctc gacgagcatg taaacctgaa caatgatgag tcccagagcc
     7081 gtgcctacaa gtacatcttt ggggtagtgc actgttgcat agatccgtgt gagacccact
     7141 actgcgacta caatgacgct gagcgcggtg aaccaggcgc gcttgacatg ccaggcagag
     7201 taagcccaga aggtggacgt tccctgcgcg tggccgcttg ggaaagacca tccggttccg
     7261 gtgtcgagca ggaagttcgg ggcatcgaga tgagggtatg caacataagg tcgaggcact
     7321 ttgaagacaa acttgaggcc cacgttgata cacgaggaga tggaggtcac catgcccaag
     7381 ttgaatccta cagacttgtc aatacaccag tagataatgg gtatcgcgat catgtagaag
     7441 gaaatggagc ccagatcggt tatgatcttt actaccctgt cgaggccggg agttgcgatt
     7501 ctcctaaaga attcaaaaac tagagagcct gtcaagattg accacctcca gctcagaatt
     7561 tcgccaaggg ggagccgaat cctctgagat gtgcatccat aggatgtgtc ccaacatatg
     7621 tgtggcggaa ggttcacatg aatggtaaaa tatagttgca aattcttctg aagggatttg
     7681 ccaatgaggg ccttatcttt ccgcacacgg tttgacgagg agaaaattgg caccataagc
     7741 gcagcgtgaa ccgaattcta tggaccatta gggtattcgc gacctttgct gtctgac
//
LOCUS       DAOSAI010000069         7743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6088_length_7743_cov_3.462149, whole genome shotgun sequence.
ACCESSION   DAOSAI010000069 DAOSAI010000000
VERSION     DAOSAI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6088_length_7743_cov_3.462149"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..980
                     /locus_tag="PLM83_03765"
     CDS             189..980
                     /locus_tag="PLM83_03765"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK70559.1"
                     /translation="MRKAQIGRFLRVGLFSLIILAMAFTGAGCTWFGPKVVVNGRVIA
                     TDANGKEIPIYRASVTIGKVSTAVTDYDGRFQISGVKAGRQTVTVQKEGFQTLTKAVE
                     VKADGSDIGDLEIVPEEGTETIMIEPEWTLLPIGSEAIAELEAKGVLDDIPEGTVIAE
                     ATIDVTLEQDGGKRYTAGARMSLERTASGVEVVDEGALIIMGIQHQAGAGYTFAVTGK
                     VGSDLDRFKQKNIAVSGSGRIEATKFEGGKSYGVTISLHGFLENR"
     gene            2421..4034
                     /locus_tag="PLM83_03770"
     CDS             2421..4034
                     /locus_tag="PLM83_03770"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOK70560.1"
                     /translation="MLSRLARKIMRAIAGDRQRGKNIISSEGDSAQLSSDLENNIAYI
                     VKAFGSSSDLVVRRLRAGKGGSVSAAVIHLDGLVDGLLVAQSVVRSITVMTESLDSPE
                     SVLDELHDGLISVTGVDKVVDGYEATLRIAEGSCVVLIDSLPTALACAVQGWEQRSPE
                     EPNTEATVRGSKEGFVESIRVNTSILRRRIAHPCLRIEECRLGRLTRTSVVIAYVQGI
                     ARPSIIQEVRSRLAAIDIDSIQESGQLEELIEDAPFSPFPTIARTERPDRIVGALLEG
                     RVAILLDGTPFALIVPVTLTMLLSTPEDYFERFYAGTLIRLIRVLSVFVSLLMPSFYV
                     AVTTYHQEMLPTSLILSIAAQREGVPFPAFLEAFIMEVMFELIREAGIRLPRVIGPAI
                     SIVGVLVLGDAAIRAGLVSPIMVMVVAATGIASLATPAFSLAIGIRVLRFAFLFLAAF
                     LGFFGIAIGALALFAYLTSLESFGVPYMSPIAPVIDAGLRDAILRVPWRAMKERPEWL
                     TWYNQTRMDSAANHKDEHSTFREGRRKEQ"
     gene            4037..6424
                     /locus_tag="PLM83_03775"
     CDS             4037..6424
                     /locus_tag="PLM83_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOK70561.1"
                     /translation="MSHKLTARQLGSILFLQRALPVTLTFPMMIGMQSSHGLWIAGAL
                     GTLISLPLLVWMASLSRAEGLDDIIQISRRRLGTTVGGAVGWLFVFYWMLLAALQVRS
                     VGEAYVIGTMPETPIVVFMVLTALVSSGIARRGIKLIAMMSELTAALILLGLLLTFTL
                     PADVMQFRNLLPLLPEDLSSLALPTGTAVSLFLDLNVLMMIAPYMKSGRDLMRGTVYS
                     ALISGAILILLAVVVTAVFGPLATSLELPALSLTRMISLGEFFERLELITVASWTSGA
                     GLVLSTSLWAAAEASANLLGLKRYEPLVYPLGGLAVIMGLGMWPNMGAFDRSASAKSG
                     SLVTAVFVIAVLVVLTGARWLNRRKGEGPGGTRMIAAILALGLTAFLATGCWSHREIE
                     SLGFVNAVGVDTALGKTHWELPGEERDPGELIQVTAHVVKPSAIVSGERGPAPEKPFW
                     VISATGYTIFEAVRNVSELSPRRLSWPHSRWVLFGEEFAKGGVARAVDFLVRDQETRR
                     RAVLGVASGARAWDLLQSEFELERVPGEAGMGIAMNASKSTSTIVIASVNDFVMALES
                     EGIDPIALRIEVMPYTYPYEITGDVTREQIKSVARLTGAAVFRSDKLVGWLDGREARG
                     YNWITGKTKSGILVIDAPELSLGQASLGSRVGLEIIRSSGSFRPKIEDNGKTIGIVIK
                     IKAEANISDVQPYVDLYAHPGLWESMERLMAKAIEDEIMAAVKKAQDLRADVFGFGRE
                     IHRTRPKLWKEIRDGWYDIFAEIEPEIEIEATLVRSGLTVRSVKLNEMGGSGGQQ"
     gene            6421..6639
                     /locus_tag="PLM83_03780"
     CDS             6421..6639
                     /locus_tag="PLM83_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70562.1"
                     /translation="MNIVLLVLAVVVLGFYEAPDLIRNKLWRELAVFVFAVGLDFTLA
                     LLMLLGVNIPNPAIWIDKAAKWMLSLFR"
BASE COUNT         1738 a         1774 c         2336 g         1895 t
ORIGIN      
        1 tatgggaagc gacaaacagg agacgcaagg agatgggaat caggcatagg gaaatcttat
       61 agtcaaagcc agtttcaaac tggtgagtgt attgagcaat caaagaggat tgggcgtctt
      121 ccttgacgaa ggcatcaatc cagtgccgaa attttggcag aagcgaagaa gtgataggag
      181 gtaatcaaat gcgcaaggcg caaattggta ggttcttacg agttggtctg ttttcattaa
      241 taattcttgc aatggcattt acaggggccg ggtgtacgtg gttcgggcct aaagtcgtcg
      301 taaatggaag ggtaattgcc acggatgcca atggaaagga aattcctata tacagagctt
      361 ctgtgacgat cggaaaggtt agcacagccg ttacggatta tgatgggcgt ttccagatct
      421 ctggcgttaa ggcggggagg cagactgtta ccgtccagaa ggaaggcttt cagacgctta
      481 ctaaggctgt cgaggtcaag gcggacggtt ctgatatcgg cgatctggaa atagtgccgg
      541 aggaaggcac ggagacgatc atgatcgagc cggagtggac attgcttccg atagggtcag
      601 aggcaatagc ggagctggaa gcgaaaggcg tgttggatga cattccggag ggcaccgtca
      661 ttgcagaggc aactatcgat gttacgctgg agcaggatgg cggaaaacgt tatacggccg
      721 gcgcaaggat gagcctggag cgaactgcct caggggtgga agtcgtagac gagggcgccc
      781 tcatcatcat gggaattcaa caccaggctg gcgcaggcta cacatttgcc gtgacgggca
      841 aggtaggaag cgatctggat aggttcaagc agaagaatat tgccgtcagt ggcagtggca
      901 ggatagaggc caccaaattc gaaggcggca aatcctacgg tgtaacgata agcctgcacg
      961 gtttcctcga aaatcgctag caacagaaga ggtaatcgca acgtgcaact tctgtctcct
     1021 ggatgcatgt tttttcgcag tgaggggatt tgatctgtcc atggctgccg cccatgttcc
     1081 tcattgactc taacgaatga cctggagagg agcgacatct tgatcatatg ggctcatgtg
     1141 gcttccccca ggattacggc aagcgtcgaa ccaggatgga gctgttaatc aaggctgatt
     1201 tggagtggga tagcgcacca gctaggcggc atagacgaaa gcctatactg tgccggatgg
     1261 tgcgcctact ctatccgcgt tctcttcaag agctctaagg cgaatgccca tctgatctaa
     1321 ctgggatcat atcgtgatca acccgctggc tcttttcgat tgcggccggg cgttgattta
     1381 gcttggcgag tttttcagta gcgaatatcc gaaactcgtg ggactcatcg aatttctcaa
     1441 gggccagggc ttgaaactga tcgagtctgt cgaacctcgc gaaggccaca gcctattggc
     1501 aaaatgcttg agcacctttt gaattgtgtc aagagcattt ggaaaaaccc catagtaccg
     1561 gcccctttca catgtagctc tgcactaagg caggctttcc ctttagtgta acctaagacc
     1621 tgtgaaaacg gggccggcct tgtttattat taccagtaat atcaagatgc ttcgtgggga
     1681 tgaagtggtc ccggggagag gctcgtacct cgtctatcca gcacctccta caccactttc
     1741 ggttactggg gtccaatctg agtaaaaatg gctttgggtg gtcatctagc gtgagccctt
     1801 acaatggggc ctgggatgag acaaccatag gcagtccgca agccggagtg gtattgccac
     1861 gtctcgccaa tatctatctt gactgctttg atcaaaaagc gaagttcaag ggaactgccg
     1921 gcaaactgat tctacgtagc cacgggatga gtgttgagga ttcatccagg agatgaaaat
     1981 ccggcggggt atgcataaag tgctccggga gcggggttgt ttagctgagt tcctgaagat
     2041 atcgatgaga cgatagaggt ttcggtcaac atgtcgtttt ttgaggcttg gtttgatggg
     2101 atagacctta tcaaatacac gaggtcgtcg tcttgcattt gttctacgaa gcgtggtgat
     2161 taaggtttgc aggagcagta tacgaggttc gtacgaacga ttctgtcaga ggtgtgatac
     2221 caggctgatc atctggcgcc atcctacctg atttgggtgg tgtcgtttct gcgagcgagg
     2281 atgcttgccc cgataattgc tcgtgcctct ggcacatgcg ggatggcagg ctctggcagg
     2341 ggagggtaga ggattatcga ataccgctgc tatcatggag aaagaatgta tacaggattt
     2401 gttggcaggg tgggaaagag ttgctttcaa gacttgcaag gaagatcatg cgagcgatag
     2461 caggtgacag gcagcggggc aagaacatca tttctagcga aggcgattcg gctcaactca
     2521 gctccgacct ggagaacaac atcgcttaca tcgtaaaggc cttcggcagc agcagcgacc
     2581 ttgttgtacg ccggcttcga gcaggaaaag ggggctcggt gtcggccgct gtcattcatc
     2641 tcgatggtct agtggacggg cttcttgtgg cccaaagcgt agttcgttct attacggtga
     2701 tgacagagag cctcgattcg cccgagagtg ttttggacga gcttcacgat ggtctgatat
     2761 cggtgacagg cgtcgacaag gtcgtggatg gatatgaggc gacgttgcgg attgctgaag
     2821 gctcatgcgt agtgttgata gactccctgc caactgcgct cgcctgcgcc gtgcaagggt
     2881 gggagcagcg cagccccgaa gagcctaaca cagaggcgac ggtgcgtggt tcgaaggaag
     2941 gcttcgtaga gtctatacgg gttaatacct cgatactgag gagacgaatc gcccatccct
     3001 gtctgcgtat cgaagaatgc cggctcggca ggctgactcg gacgtcagtg gtaatcgctt
     3061 acgttcaggg aatagctcgt cccagcatca ttcaagaggt gagatcgagg ctagcggcga
     3121 tcgatatcga ctcgattcag gaatccgggc aattggagga actcatagaa gatgctccgt
     3181 tttctccctt tccaactata gcccgaaccg agcgacctga cagaattgtt ggagctctcc
     3241 ttgaggggag ggtggccatc cttcttgacg ggaccccatt tgcgctgatc gttcctgtta
     3301 ccctcacgat gcttctgtca actcctgaag actatttcga acggttttac gctgggacac
     3361 tgatcaggtt gatcagagtg ctatctgtct tcgtctcgct tcttatgccc tcgttctatg
     3421 ttgcagtaac aacctatcat caggagatgc ttccgacttc tttgatcctt agcattgccg
     3481 ctcaaagaga aggcgttcct tttcccgcct tccttgaagc cttcataatg gaggtcatgt
     3541 ttgagctgat aagggaggct ggcatacgcc ttcctcgtgt cattggcccg gccatcagca
     3601 tagtgggggt gttggtgttg ggtgacgctg ccattcgagc aggtctcgta tcgccgatca
     3661 tggtaatggt cgttgcagca actggcattg catctctcgc aacacccgca tttagccttg
     3721 ctataggaat cagggtgctt cgtttcgcct tcctctttct cgcggctttt cttggctttt
     3781 tcggaatagc cataggcgcg cttgccctct tcgcttacct cacatccttg gagtctttcg
     3841 gggttcccta catgtcgccc atcgcgccgg taattgatgc aggacttagg gacgcgattt
     3901 tgcgggtccc gtggcgggcg atgaaggagc gtccggaatg gctgacttgg tacaaccaga
     3961 cgaggatgga ttcggcggca aaccacaagg acgaacatag cacgtttcgg gaaggccgca
     4021 ggaaggagca atgactatgt cccataagct gaccgcgcgc cagttagggt cgattctttt
     4081 cttacagagg gctttgcctg tcactctgac gttcccaatg atgataggca tgcagtcttc
     4141 ccatgggttg tggatagcag gggccttggg tactctcatc tccctgcctc tgctggtgtg
     4201 gatggcgtcg ctgtcaaggg cggaagggct tgatgacatt attcaaatat cccgccgccg
     4261 tcttgggacg actgtaggtg gagcagtagg ctggctcttc gtcttctatt ggatgcttct
     4321 tgcggctttg caagtgcgct cggtgggaga ggcttatgtg attggtacca tgccggagac
     4381 tcctatcgta gtcttcatgg ttctgacggc gctggtcagt tcgggcatag ctcggcgcgg
     4441 cataaagttg attgctatga tgtcggagct tacagccgct ttgatccttc tgggattgct
     4501 gcttacgttc actcttcctg cagacgtcat gcaatttaga aacctgttgc cgcttcttcc
     4561 tgaagacctg agttcattgg cgctcccgac tggaactgca gtcagtctct tcctcgacct
     4621 taacgtgctc atgatgatag cgccctacat gaaaagcggc cgggatctta tgagaggcac
     4681 agtctattct gccctgataa gcggcgctat cctgattctt cttgcagtgg ttgttacagc
     4741 ggtcttcggg cctcttgcca cgtcacttga gttgcctgcc ctcagcctta cccgcatgat
     4801 cagccttggg gaattctttg aaaggctgga gctgattact gttgcctcat ggacctccgg
     4861 ggcgggcctt gtgctttcca catcgctttg ggcggcggct gaggctagtg cgaatttgct
     4921 tggcttaaag cgctacgaac cactagtcta ccccctggga gggttggcgg tgatcatggg
     4981 attgggtatg tggcccaaca tgggagcatt tgacaggtcc gcttctgcca agagcgggag
     5041 ccttgtcacg gcggtcttcg ttattgctgt gctggttgtg ctgactggag caaggtggtt
     5101 gaatcgacgg aaaggcgagg ggcccggcgg gacgagaatg atagcggcaa tactagctct
     5161 agggcttact gcatttcttg ctacagggtg ttggagtcac agggagattg agagcctggg
     5221 atttgtcaac gcagtgggag tagataccgc attgggcaag acccactggg aattgccggg
     5281 tgaagaacgc gatcccggcg agttgattca ggtcacggcc cacgtagtca aaccttccgc
     5341 tatagtcagt ggcgaacgtg ggcctgcacc tgagaagccg ttttgggtaa taagcgcgac
     5401 aggatatacc atctttgagg cagtgcgcaa tgtgtcggag ctttcgccgc gcaggctctc
     5461 ctggccgcac agtagatggg tgctattcgg cgaggagttc gcaaagggcg gagtggcaag
     5521 ggcggttgac tttttagtgc gcgaccaaga aactcggcgg cgagccgttt tgggcgtggc
     5581 ctcaggcgct agggcatggg atctgctcca atcggaattt gagttggagc gagtgccagg
     5641 ggaagcgggg atgggcattg cgatgaacgc ttcgaagagc acgtcgacta ttgttattgc
     5701 ctcagtcaat gacttcgtta tggcgttgga atcggaagga attgatccaa tagctttgcg
     5761 catagaggtc atgccgtata cgtatcctta cgagatcacg ggagacgtca cacgggagca
     5821 gattaaatct gtggctaggt tgacgggcgc agcagtattt cgtagcgata agctggtagg
     5881 atggctcgat ggacgcgaag ccagaggata caattggata acgggaaaga ccaagtccgg
     5941 gattctggta atagatgcgc ctgaactgag cttgggtcag gcttcgttag ggtctcgcgt
     6001 tggacttgaa atcatcagat ctagtggatc cttcagaccc aaaatcgaag acaatggaaa
     6061 aacaataggg atcgtcatta agataaaggc agaggctaac atatcggatg tgcagccgta
     6121 cgtcgacctg tacgctcacc ctggactgtg ggagtcgatg gagcggctga tggcaaaggc
     6181 gatagaggat gaaatcatgg ccgctgtgaa gaaggctcag gatcttcgag cagatgtatt
     6241 cggttttggg cgcgagatcc atcgaacccg tccgaaactt tggaaagaga tccgggacgg
     6301 ttggtacgac attttcgctg aaatagagcc ggagatcgag atcgaggcaa cgctggtcag
     6361 gagcgggctg actgtgcgca gcgtcaagct gaatgaaatg ggaggatcgg gtggacagca
     6421 gtgaatatag tgctgctagt cttggcggtc gtggttttgg gattctatga ggcgccggac
     6481 ctcataagga acaagctctg gcgggagctg gccgtgtttg ttttcgctgt agggctcgac
     6541 ttcacgctgg cgcttttgat gttactcggg gtgaatattc cgaatccggc gatctggata
     6601 gacaaggccg caaagtggat gctttctctc ttccgttagg gaggtgtcgg ttgcaggcca
     6661 agcacggtca gtgttttgag aggccaaaca gccggtatgg cacgattcgc cattcgttcc
     6721 agccaaagac gcattcaagt tgtttgtctc atgcctatcg gatctatgtg ccaggcgaat
     6781 tttggtatag gcctgtaaaa actcttaggc acgcctggca agctctatat gaggagagaa
     6841 gaggaatacg gtcgcaggtg gagaatgacg cagcgagcgc gcaggctcat aaatcgtctc
     6901 cagaacctgt atgatgcgcg ccttgccgcg cagaaaatcg actcggtctc agagtggcag
     6961 agcacattaa tggggtcaat gatcaagtgg agaagttgag aatgttttct gagaccaccc
     7021 gaccggtgct ccgcattcaa ccttgggatg agctattgcc tcatgtgccg actgttttag
     7081 agctgagatc cctgcctttt gggacaagtc ttccgagcac gaaggtcctg acaagcttgg
     7141 ctaggtcatt gataggggca cataaggcac ctgcgctggc gtttactgca attcaggcgg
     7201 tttcgcctat gctgttcgtt agatcgaaca cgtggaaagc cgtacccgaa ggaatggtcg
     7261 ggctttacgt cacgacaagg gcgtccttgc attggatgtg ggattatcct ctcgggactc
     7321 gaatgccgac gacatgatat tcccatgctg gtacagaaaa tttccaagtt tattcggaga
     7381 agtgcgaacg tgaggtgtga gccacataaa gcaggctacc caaaggcctc tttctgcaag
     7441 cagctgcaga cgatatctgc tctaagcatg accggtatgc cgtatgtgct ccatctagcc
     7501 actgaactct gccatattct atgaaacacg ttgcccgata gcgtttcccg aatcggtgcg
     7561 agaacagccc gttctcgcgt aggtttgaca ttgtcagcgt tgggcaagaa tgtgggcgga
     7621 tgctccagtt gaagctgtac aaggggagat ggtcggatat catggtgggc agagctctgc
     7681 tctttgcagg attggtggcg gttgcggggt acgtggtggt cactgtggtc ggagcggaga
     7741 tga
//
LOCUS       DAOSAI010000070         7695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6131_length_7695_cov_3.722120, whole genome shotgun sequence.
ACCESSION   DAOSAI010000070 DAOSAI010000000
VERSION     DAOSAI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6131_length_7695_cov_3.722120"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2443
                     /locus_tag="PLM83_03785"
     CDS             <1..2443
                     /locus_tag="PLM83_03785"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOK70563.1"
                     /translation="SGKLLPSEADLDVAARLAARGVGFWIVNNDVSQIRSVTDPLANQ
                     AGSIVIDPADIRHIFSLDGPLSAAFEDYEVRQGQIDMALAVSSAFSDSACLVVEAGTG
                     TGKSLAYLVPAFLWAKRNNARVVVSTNTINLQEQLIQKDIPALEAAIGEPLPAALVKG
                     RGNYLCMRKLNRVLEDEESSIEPEYRDGFAQVLQWAMETETGDRSDMQFEPDSEVWGM
                     VCSEGDMCLRTKCPNYGQCCFHRARQAMEKAEVLVVNHHILFADAFIRSQMGPEADRA
                     VLPRYNVVIVDEAHNAADVATQHFGRRVSRVSLVRLTNSIYRRERAAAQSVGTASTGD
                     YGALVRLRSSLYSSWPGESGRARAEACLDLIHIDAIPAVIRVREAGEAFFDEVRRMLM
                     DVPGDGVERTLRITGDVKQREDWDARIFPAHERFAAALEGLADCLERLSVLLRGDDDE
                     ALLESAKRLTFEAESGSGVAADEDGVDLGPGFDEADFPDIVELAAYAGRAREFASSLR
                     FAVTADAPGHVFWAEASGAGPRSNVRVVAAPIEAGKAIAEHVLSMQESVVFTSATLAV
                     GTSFDYIIHDLGLDLLDGDRVRQMAIAAPFDYGRQALLAVVDDIPNPDSHGWEAAVAE
                     AVASILDASDGRAFVLFTSYKLLSEVARAVEPRLAQRNIALLRQGNAPRHALLAEFRR
                     DVRSVLFGTDSFWEGVDVPGEALSCVVMPRLPFAVPTAPVVEARIEHIAESGGNPFNQ
                     YTLPAAVLKFKQGFGRLVRSSSDRGAVVVLDSRIVRRHYGRKFIDALPGCAKYVGGFE
                     DVSERLYEWLINPSP"
     gene            2621..3487
                     /locus_tag="PLM83_03790"
     CDS             2621..3487
                     /locus_tag="PLM83_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOK70564.1"
                     /translation="MVFFRILSKWRTFLDVACVAFVLSFVLTLFGNATAEVGMGVISA
                     VSTGGRLVPIYSVARDDKTVSVTVDATWGDDKTLALLDLFDKHEIKVTFFLAGNWIKS
                     YPEKVKAIAARGHEVGNHSWTHPHMGGLSSAQIREELERTQSALCDLTGVRPTVFRPP
                     FGEYTNSVITIAEECGCKTIQWSIDSLDWKDLTADQIYDRVMKRVGPGSIILFHNAGK
                     HTPEALDRIIRDLKAQGYAVVPVSELIYSSDYYIDRNTGEQRPVKVRTRAEPSLPQQS
                     PPTSDSSSGLRR"
     gene            3583..4581
                     /locus_tag="PLM83_03795"
     CDS             3583..4581
                     /locus_tag="PLM83_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOK70565.1"
                     /translation="MIFITIRRRTLILIVLGVILAIVAAYIVVSMPRMRRAALGAKPG
                     VRVRDVDVGGLYKEEVRSIVLGLAGKVERSPKEAMYHAETGEIVPEEPGIIVDIDNTV
                     TSVMAAPRGAVLDLVTRQVPPAVTAAMLKPIYRVDTSEPAVALAFNVAWGEEWVPAIL
                     EILAEAGAKSTFFITGTWAAKFPHLVQAMVAAGNEIANHGYDHPHPEKLSESELVALI
                     EKNETLLRELTGVRTRLFAPPYGEVNQRIAAVAGRLGYATIMWTVDTIDWQRPEPGVI
                     VERAERRLAGGNIILVHPTEPTVAALPVILERFAEKGYKAVTVSRLLELGPAVHTR"
     gene            complement(4595..5932)
                     /locus_tag="PLM83_03800"
     CDS             complement(4595..5932)
                     /locus_tag="PLM83_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOK70566.1"
                     /translation="MQPNTTATALVILMYIASSIAGMRTNKTNPGVQTPPGIQTTSPT
                     PQTEKNAVLDLASRSAAISETANVRSGPGTNYPKTGKLAKNTPVRLVSQANGWYQVSL
                     PNGGYGWVAGSLLRIGQPSGAPAQLKHDVVGYYTVNYPGDKSSYNVLDSHSRSLTAIA
                     PFSYAVDRAGNVTGEHFHDAMEIASSRGLSNLALVHNLNGKSFNQSDIHSMLNSKAAR
                     ERAIRDIYDLVKKNGYDGVNIDFENVPPGDRRVLTQFMQELRSKLGPAGYKVTMSVPA
                     KYKDSPSSAWIGAFDYYALGKVCDQIMLMTYDQHTSGTGPGPVASVQWVEKVIKYAMT
                     QMPKKKILVGIAAYGYDWNTSTNRAKAISYSQAIRTAERHGTKISWDNASQSPYFRYK
                     SGNVTREVWFESADSMSAKLKLVKKHDLGGIAIWRLGQEDAGYWKVIERELQN"
     gene            6331..6795
                     /gene="dut"
                     /locus_tag="PLM83_03805"
     CDS             6331..6795
                     /gene="dut"
                     /locus_tag="PLM83_03805"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOK70567.1"
                     /translation="MPVIDERRPCPSLPGYMSGSAAGIDLMACLEDNVELAPGEYRMI
                     PTGLKMAIPEGYEGQVRPRSGLAAKFGITVLNSPGTIDADYRGEIGVILINHGFEPYT
                     IANGDRIAQLVIAPVVKVELLETAELDETERGEGGFGSTGVSDVDGKRCTDG"
     gene            6788..6985
                     /locus_tag="PLM83_03810"
     CDS             6788..6985
                     /locus_tag="PLM83_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70568.1"
                     /translation="MAEAIAARLEQLKTEIEELRAELRETATREGAIADLDRACSVSD
                     RLDALILDFLRVKDQVGHTVD"
     gene            7153..7638
                     /locus_tag="PLM83_03815"
     CDS             7153..7638
                     /locus_tag="PLM83_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70569.1"
                     /translation="MSKVIGTFASRDAAEFCIRELRNSGFDYNRISLVARESGKGGEQ
                     QGGGPTMAGGDAALEGTATGGAIGGMAGLLAGLGALAIPGIGPIVAAGPIAATLTGAV
                     GGGLVGGLVDMGIPSERGEFYEGKVKAGRILAVVDAGSDKVNDAARIMREYGASDVET
                     H"
BASE COUNT         1623 a         1947 c         2339 g         1786 t
ORIGIN      
        1 gtctgggaag ctcttgccgt ccgaagctga tttggacgtt gccgcaagac tggctgcccg
       61 gggcgtaggc ttctggattg taaacaacga cgtctcccag atacgctcgg ttactgatcc
      121 tctggcgaat caggcgggct cgatcgtgat tgaccccgcc gatatcaggc acatcttctc
      181 gctagacggc ccgttgtcgg ctgcgtttga ggattacgag gtgcgccaag gccagataga
      241 catggcgctc gcggtttcgt cagcattctc cgactccgcc tgccttgttg tcgaagccgg
      301 tactggaacc ggcaagtcgt tggcatacct cgttcccgca tttctttggg cgaagcggaa
      361 caacgcccgc gtcgttgtat ctacgaatac gatcaacctt caagagcaat tgatacagaa
      421 ggatatacct gcgcttgagg cggctatcgg agagcctctc cccgctgcgc tcgtgaaggg
      481 acgcggcaac tatttgtgca tgagaaagct caatcgcgtg ctagaggacg aagagtcttc
      541 gatcgaacct gaataccgcg acggatttgc ccaggttctc caatgggcga tggagaccga
      601 gactggggat agatcggata tgcaattcga gcctgactcc gaggtgtggg gcatggtatg
      661 ttctgaaggt gatatgtgtc ttcgaacgaa gtgtccgaac tacggccaat gctgtttcca
      721 cagggcaagg caggcaatgg aaaaagcgga ggtgttggtg gtaaatcacc atatcctctt
      781 tgcagacgcg tttatcagaa gccagatggg tcccgaagcc gatcgggcag ttttgccccg
      841 ttacaacgtg gtgatcgtcg atgaagccca caatgctgcg gacgtcgcca cacagcattt
      901 cggcaggcgg gtatcgcggg tttcccttgt gcgccttacc aacagcatat atcgtcgtga
      961 acgcgctgca gcgcagtctg ttggcacggc ctccacaggt gattacggcg cgcttgtgag
     1021 gcttcgttcg agcctgtatt cttcatggcc cggggagtca ggccgtgcgc gagccgaggc
     1081 ttgcctcgac ttgatccaca tcgacgcaat tcccgctgtc attcgagttc gagaggcggg
     1141 ggaggctttc ttcgatgaag tccgccggat gctcatggat gtgccggggg acggcgttga
     1201 gcggacactt cggatcaccg gggatgtgaa gcaacgcgag gattgggatg ccagaatctt
     1261 tcctgcccat gaacgttttg ccgcagcttt ggaggggctt gcagactgcc ttgagaggct
     1321 ctctgtgctt cttaggggtg atgatgacga ggcattgctg gaaagtgcta agcggttgac
     1381 gtttgaggcc gagtcaggat ctggggtggc tgccgatgaa gatggagttg acctgggtcc
     1441 gggttttgat gaggctgact tccctgacat tgtggagctt gcagcctacg ccggccgggc
     1501 tagggagttt gcctcctcgc tccggtttgc ggttacggct gacgcgccgg gccatgtctt
     1561 ctgggcggag gcttccggcg caggcccgcg atcgaatgtg cgggtggtgg ctgcgccgat
     1621 tgaagcgggg aaagccattg ccgagcacgt gttgagtatg caggagtccg tggtcttcac
     1681 ttctgcgact ttggccgtgg gaaccagctt cgactacatc attcacgacc taggcctcga
     1741 tcttttggat ggtgaccgcg tccgacagat ggcgatcgcg gcgccgtttg attacgggcg
     1801 gcaagccctt ctggccgtgg tggatgatat tcctaaccct gactcgcacg gatgggaggc
     1861 ggctgttgct gaggccgttg cgtccatctt ggatgcgtct gatggtcggg cattcgtcct
     1921 gttcacttcg tacaagctac taagtgaagt cgcgcgggcg gtggagccac gccttgccca
     1981 gcgtaacatc gcgcttcttc gtcagggaaa cgcgccgagg cacgcgctct tggccgagtt
     2041 taggcgggac gttcgttctg tgctctttgg caccgatagt ttctgggaag gtgtggatgt
     2101 cccgggcgaa gcgctctcgt gcgtcgtcat gcctaggctg ccattcgccg ttcccacggc
     2161 gccagtggtg gaggcgagga tagagcatat tgccgagtca ggcggaaacc catttaatca
     2221 gtacacactt cccgctgcag ttctcaagtt caagcaggga ttcgggaggc tagtgcgttc
     2281 gtcgagcgac cgcggagctg tggtcgttct cgattcacga attgtccgac gccactacgg
     2341 ccggaagttc attgacgcgt tgccaggctg tgcgaaatac gttggcggct ttgaagatgt
     2401 tagcgagcgg ctttatgaat ggctcatcaa tccctcacct tgaggagata gaccgcagtc
     2461 aggttagaac tcgattttcg cgaccatggc agtctcttcc cccatgccct ccgaggcctc
     2521 gccgcgtcat gatctcgcgg cactctacat tatctcatat tctatacccc gtccctttgc
     2581 atatgtatta gtggcaacaa tcgcgagaag ggtgatttca gtggtgttct tccgtatatt
     2641 gagcaaatgg cggacgttcc ttgacgtggc atgtgtggct tttgttttat ctttcgttct
     2701 aaccctcttt ggaaacgcta ctgccgaggt gggtatgggt gtgatctctg cggtgagcac
     2761 aggcggcaga ctcgttccca tatactccgt tgctcgcgac gacaagaccg tatcggtaac
     2821 ggtcgatgct acatggggcg acgacaagac actggctctc cttgacctct tcgataagca
     2881 cgagatcaag gtcacattct tcctggcagg caactggata aagtcttatc cggaaaaggt
     2941 caaggctata gccgcgcgcg gccacgaggt gggtaatcac tcctggaccc atccacacat
     3001 gggtggactg tcatctgcac aaatcaggga agaactggaa cgcacccaat ctgctctgtg
     3061 cgacctgact ggggtgaggc ctaccgtgtt tcgtcccccc ttcggcgaat acacgaactc
     3121 cgtaataacc atcgccgaag aatgcggatg caagacgatt caatggagta tcgattctct
     3181 agattggaaa gacctgactg cagaccagat ttatgatcgg gtaatgaaac gggtggggcc
     3241 tggatcgata atcctctttc acaatgccgg gaaacacacg ccggaggcgc tcgacaggat
     3301 aatccgggat ctcaaggccc aaggctacgc ggtggttccc gtatcggagc tcatctactc
     3361 aagcgactac tacatagatc gaaacaccgg ggaacaaagg ccggtcaagg tgagaacccg
     3421 cgcagagccg agtttacccc agcaaagccc accgacatct gattcgtcat cggggttgcg
     3481 tcggtagagg aagtggcggg caatgaacga cgaaagcctg taggaatcga tgatgcgagc
     3541 ggagagcgcc caatccaaat agagggtggg aggaacggcg acatgatttt catcaccatt
     3601 aggcgacgga cgcttatcct catagtcctc ggggtcattt tggctatcgt ggctgcgtat
     3661 atagtggtaa gcatgcctcg aatgcgtcgg gctgcccttg gagcaaagcc tggggtgaga
     3721 gtgcgcgatg tggacgtggg cgggctgtac aaagaagaag taaggagcat cgtgttgggg
     3781 ttggcgggca aggtcgaaag aagccccaag gaagcgatgt atcatgctga gacaggcgag
     3841 atagttccgg aggaacccgg cattatagta gatatcgaca acacggtgac gtcagtcatg
     3901 gcggcgccgc ggggggccgt tttagacctg gttacgcggc aggttccccc cgcggtaacc
     3961 gccgcgatgc tcaaaccgat atacagggtt gatacgtcgg aacccgcggt ggctttggca
     4021 ttcaacgtgg catggggaga agaatgggtt ccggcaattc ttgagattct tgctgaagct
     4081 ggcgcgaaaa gtacattctt cattaccgga acatgggcag caaagtttcc ccatttggtc
     4141 caggcgatgg tcgcagcagg gaatgagatt gccaatcatg gctatgatca tccgcaccct
     4201 gaaaagctct ccgagagcga gttagtggcg ttgatcgaga agaatgagac tctcttgaga
     4261 gaactcactg gagtgcgcac caggctcttt gcgcctccct atggagaggt caatcagaga
     4321 attgcggctg tggcgggccg attgggttat gccacgatca tgtggactgt tgacaccata
     4381 gattggcagc gcccggaacc gggggtcata gttgaaagag cggagcggcg gttagcaggg
     4441 ggaaacatta tcttggtcca tcccaccgag cctactgttg cagcgcttcc cgtgattctg
     4501 gagcgatttg cggaaaaagg ctacaaggca gtgactgtgt cacgcctcct cgagttaggc
     4561 ccggcggtac acacgaggtg aaccgcacgc cccgctagtt ctgcaattcc cgttctataa
     4621 ccttccagta acccgcatct tcctgcccga gtcgccatat ggcgatcccg ccaaggtcgt
     4681 gctttttgac aagcttcagc ttggcgctca tgctatctgc gctttcaaac catacttccc
     4741 ttgtcacgtt gcccgacttg tagcggaagt atgggctctg tgaagcgtta tcccatgaaa
     4801 tcttcgtccc gtgtcgttct gccgtcctaa tggcctggct gtaggagatc gctttcgcac
     4861 ggtttgtact ggtgttccag tcatagccgt aggcggcgat gcccaccaag atctttttct
     4921 tcggcatctg tgtcatggcg tacttgatca ccttttctac ccattgcaca gacgcgacag
     4981 gccctggccc cgtgccggac gtgtgttgat cgtaggtcat cagcattatc tgatcgcata
     5041 ctttgcccag ggcgtagtag tcgaatgcgc caatccatgc agatgacgga gaatccttat
     5101 acttcgctgg caccgacatt gtcaccttgt atcccgccgg ccccagcttc gacctgagct
     5161 cctgcatgaa ctgggtcagc actcttctgt ctccaggagg aacgttttcg aagtcgatat
     5221 tgactccatc atatccattt ttcttaacaa gatcatagat gtccctgatc gcccgctctc
     5281 tcgcagcctt cgagttgagc atcgaatgga tatcgctctg gttaaacgat ttgccattaa
     5341 gattgtggac aagagcgagg ttgctaagtc cacgggatga ggctatttcc atggcgtcat
     5401 gaaaatgctc gccagtgacg ttcccggctc tgtccacggc gtatgaaaac ggcgctatgg
     5461 ccgtcagcga ccttgagtgg gagtcgagga cgttgtatga cgacttgtcg cctggatagt
     5521 tcacagtgta gtatcccaca acatcgtgct taagctgcgc gggcgcacct gagggctgtc
     5581 caattcgcag caatgatccg gcgacccatc catagcctcc gtttgggagc gatacctgat
     5641 accacccgtt ggcctggcta accaggcgca caggggtgtt cttggcgagc ttgccggttt
     5701 tggggtaatt cgttcccggg ccggaacgga cgttggccgt ttccgaaatg gctgcgctgc
     5761 ggcttgcaag atccagtact gcgttttttt ctgtttgggg ggtgggtgat gtagtctgta
     5821 tacccggagg cgtttgaacg cccggatttg tcttgttggt gcgcatgcct gcaatagagc
     5881 ttgcaatgta catgagaatg accaatgctg ttgcagtggt gttaggttgc accgggcgcc
     5941 ctcctctcca ggtagacgta atattgtatc gtctgccgca gcgcagctga gagaggattt
     6001 cgtcgctgcc tccttatttc cttcaagtaa ataggggctg gaatagagat agctggaaat
     6061 atcaatcgac cgttagcgca cgaaaaaggc tgtcggagca cacatctttt tcgatgtgtt
     6121 tatccgacct tcgcaggact tctgctcggc agaaagaatt caaatctgtt ggggatgcgg
     6181 tatctcggag gtttttgcct ccctttgcca caaatcttcg catattgcac gtgtcaagga
     6241 agtgaattct aattgtcaga acgccaagac tgctatggag aaagctctaa gctttgcaac
     6301 gctcaggact cgcgtcctgt tgtaagggtg atgcccgtta tagatgaacg gaggccttgt
     6361 ccgtcacttc caggatacat gagcgggtcg gctgcgggaa tcgatcttat ggcgtgcctt
     6421 gaggataacg tagagcttgc gcccggagaa tacaggatga ttccgacagg cctcaagatg
     6481 gctattcccg aagggtatga gggtcaggtg cggcctagaa gtgggctggc tgctaagttt
     6541 gggatcacgg tccttaattc gcctggaacc attgatgcag attaccgcgg cgaaatcggt
     6601 gtgatactaa taaaccatgg atttgaaccg tacactatcg ccaatggcga ccgaatagcg
     6661 cagcttgtga tcgctccagt tgtcaaagtg gagctcctgg aaacagctga gctggatgaa
     6721 acagagcggg gagaaggcgg attcggaagc acaggcgttt ccgatgttga tggaaagagg
     6781 tgcactgatg gctgaggcca tagcagccag acttgaacag ttgaaaacgg agatcgagga
     6841 attgagggcc gagctgcgtg agacagcgac gcgtgagggt gccatagccg atctggacag
     6901 agcatgttca gtatcagatc ggctggacgc gcttatcctc gacttcctta gagtgaagga
     6961 tcaggttggt cacactgttg attgataagc gtcactcgat tgccatagcg tccccgagtc
     7021 cctgcggcga aggattcgaa tctaaggata tcgcctgcgc gcagcggcag aagcggttca
     7081 gaatgaacca tgacctcctg gttaaactaa gcgcagtgct caagcttttg caggatacaa
     7141 ggaggtcgaa tcatgtcgaa ggtaataggc actttcgcgt cgcgggacgc cgcggaattc
     7201 tgcattcggg aactgcgaaa ctcgggtttt gactacaacc ggatatcgct ggtggctagg
     7261 gagtctggaa aaggcggaga gcagcagggt ggcgggccca ccatggctgg gggcgacgct
     7321 gcgctggagg gtaccgctac cggtggagct attggaggaa tggcaggtct cctcgcgggc
     7381 ctgggcgcct tggcgattcc ggggatcgga cctattgtgg ccgccgggcc tatcgcagcg
     7441 acccttactg gggctgtagg aggaggcctg gttggaggat tggtcgatat gggcatcccc
     7501 tcagagaggg gagagtttta cgaagggaag gtcaaagcgg gccgaattct agccgtggtc
     7561 gatgcgggtt ctgacaaggt gaatgatgct gcgcgcatca tgcgcgagta cggcgcgtct
     7621 gacgtagaaa cacactaagg aaggcggctc tctggaaggg tggcatatct cagcagaact
     7681 gggggagcgt ccagg
//
LOCUS       DAOSAI010000071         7617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6220_length_7617_cov_3.761307, whole genome shotgun sequence.
ACCESSION   DAOSAI010000071 DAOSAI010000000
VERSION     DAOSAI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7617
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6220_length_7617_cov_3.761307"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLM83_03820"
     CDS             complement(<1..611)
                     /locus_tag="PLM83_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOK70570.1"
                     /translation="MLEGRVDLHCHTTKSDGTFTPTELVGEAARRGLAAVAITDHDCV
                     DGIGEALEAVEENCVDVVPGVEISTDVSDGEIHILGFFIDYEDEDLLKFLEAQRESRV
                     RRVERMLRKLAALGIDLSMEDVLEHAGDGAVGRPHIAAAMVKAGYVLSWDEAFAHYIG
                     LHAPAYESRSKLTPYEAIEAVLAAKGVPVLAHPGLSGKDDMIPS"
     gene            complement(592..3714)
                     /gene="ileS"
                     /locus_tag="PLM83_03825"
     CDS             complement(592..3714)
                     /gene="ileS"
                     /locus_tag="PLM83_03825"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004443517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOK70571.1"
                     /translation="MFKKVDSAMDFPRMEEEILEVWREEKIFEKSVDRREKGPRFVFY
                     EGPPTANGLPHPGHVLTRAMKDVVLRYKTMKGFYVPRKAGWDTHGLPVELEVEKELGI
                     SGKPQIEEYGVEEFVQRCKESVFKYESEWRRMTERLGFWIDMDDPYVTFHDQYIESVW
                     WAVKTIWDKGLMYHGYKVVPYCPRCGTALSSHEVAQGYKEVEETSIFVKFKVKGEENV
                     SLLAWTTTPWTLPSNVALAVSADYDYAYVERDGETLILAKDLVESRVKGDYRIVRVVK
                     GSELMGMEYVPLYTYENPEAPAYMVVEGDFVTLSEGTGIVHIAPAFGEDDMRVAQNNG
                     LPIIQLVDSQGRFDDRARDWSGMFVKDADPLIVQDLKARGLLFEEMDYTHSYPYCWRC
                     DTPLLYYARGSWFIRMSDLREQLLANNAKISWYPEYIRDGRFGNFLENAIDWAVSRER
                     YWGTPLPIWVCEECGHEHAVGSVEELKSMAKSLPDRLELHRPYIDQAVLACPECGGDS
                     KRVPEVMDCWFDSGSMPFAQWHYPFENAQAFSAAFPADFITEAVDQTRGWFYTLLSIS
                     TLLFDRPPFENCVVLGLVLDEAGLKMSKSKGNVVDVWKLFDDHGADAVRWYLYTVNAP
                     WNPTRFYEDGVIEAKRKFMGTLWNVYAFYVLYANIDEFDPSQYHVAPEDRSVIDRWLL
                     SKLSGLVGEVTEAMDCYDITAAARRIEQFVDDLSNWYVRRCRRRYWASEMTVDKQAAY
                     LTLHETLTTIAKLIAPFTPFLAEEIFTNLEPGSGSVHMQDYPKADEWARDVDLERQMD
                     LARQVVFLGRAARNEVNIKNRQPLLKVVVGATPEEKKDLRLVEDLVKDELNIKRLEYA
                     QDMSEFLSYRVKPRYDLLGPKHGKLMKAIAEGIAEADASQIAGKVLKGREFGLVLSDG
                     TEVSILPEEVSIQTVAAEGYYVQSEGGRAIALDTVITEELKLEGFARELVNKIQTTRK
                     EAGFDVSDRICTVILGGPDSMRALEVHRDYLMKETLSVSLAHGEPEGEYVREWDVNGE
                     KVAIGVRRAS"
     misc_binding    complement(3790..4051)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4174..4752)
                     /gene="thiT"
                     /locus_tag="PLM83_03830"
     CDS             complement(4174..4752)
                     /gene="thiT"
                     /locus_tag="PLM83_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOK70572.1"
                     /translation="MSTTRSRKPTPTIVLTEVGMAAALSWVLGMIRLFKMPQGGSISL
                     EMVPILYMAFSRGVYPGLLSGLIAGVLDLVTATSEIIHPMQAILDYILASTALGLAPW
                     FSFGRGWWRNLTGAIGGTLVGLMCHVLSGHIFFGQYAPAGTPAWLYSLTYNATYYLPK
                     MIITIVVVVILMQKGVLRPENGGRGNGNRSQG"
     gene            complement(5094..5297)
                     /locus_tag="PLM83_03835"
     CDS             complement(5094..5297)
                     /locus_tag="PLM83_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70573.1"
                     /translation="MEVTNIKGVLVATLVMDEGDADEIGTMSESEVIRRARKAVGTVI
                     PEHLIREILVGHDGDSLHVAFSI"
     gene            complement(6059..6592)
                     /gene="spoVT"
                     /locus_tag="PLM83_03840"
     CDS             complement(6059..6592)
                     /gene="spoVT"
                     /locus_tag="PLM83_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HOK70574.1"
                     /translation="MKATGIVRRIDDLGRVVVPKEIRRTLRIREGDPLEIFTDREGSV
                     ILKKYSPIADLGEFAQEYADSLYEATRHIVLIADQDEVVAVAGASRKSFYERRITEHV
                     EEVMRNRKPFLSNNANENPPIEDLELSSQVIAPIIVEGDIAGAVIMLSQEGKSMGELE
                     LKLAETAAGFLAKQLGQ"
     gene            complement(7374..>7617)
                     /locus_tag="PLM83_03845"
     CDS             complement(7374..>7617)
                     /locus_tag="PLM83_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOK70575.1"
                     /translation="FSEVDDKETELIRRSRYTNDLHCRLIALMEQVEEIAEAGIDDDL
                     DPGCVAVFKRAREEAWIIGQSAKAEIRAHIAKGKWS"
BASE COUNT         1766 a         2335 c         1677 g         1839 t
ORIGIN      
        1 gaaggtatca tatcgtcttt tcctgaaagg cctggatgag ccagaacagg cactcctttc
       61 gcagccagaa cggcctctat cgcctcatag ggagtcaatt tggacctgct ttcataggct
      121 ggcgcatgaa ggcctatgta atgcgcaaac gcctcatccc aactgagcac gtagcccgct
      181 ttgaccattg cagccgctat atggggcctg ccgactgcgc catcacccgc atgctctaac
      241 acgtcttcca tgctcagatc gattccgaga gccgcaagct ttctgagcat ccgctccaca
      301 cgccgaaccc tgctctctcg ttgtgcttcc aggaatttca gcaaatcctc gtcttcgtag
      361 tctatgaaaa accccagtat gtggatctcg ccatcactga catcagtgga tatctccacg
      421 ccgggcacaa catcaacgca attctcctca accgcctcaa gcgcttcccc tatgccgtcc
      481 acacaatcat ggtctgttat ggcaacggcg gcgaggccgc ggcgggcggc ctcgccaacc
      541 aactcagtgg gcgtgaacgt accgtctgac tttgtagtat gacagtgcag atcaactcgc
      601 ccttctaaca cctatagcca ccttctcgcc attgacgtcc cattcacgca cgtactcccc
      661 ttcaggctcg ccatgggcaa gcgaaaccga gagcgtctcc ttcatgagat aatccctgtg
      721 aacctccagc gcgcgcatgg agtcgggccc acccaagatc actgtgcaaa tgcggtcaga
      781 cacgtcaaat cccgcctcct tgcgggtcgt ctggatcttg tttactagct ctcttgcgaa
      841 cccttctagc ttgagctcct cagtgatgac tgtgtcgagg gctatcgctc gtccgccctc
      901 ggactgtacg tagtatccct cggcagcaac agtttgaatg gacacttctt caggaaggat
      961 ggatacctca gtcccatcgc tcaagaccaa cccaaactcg cgccctttta aaacctttcc
     1021 agctatctgc gaagcatctg cttcggcgat gccttcggca atcgccttca tcaacttgcc
     1081 atgcttcggt cccagaagat catatcttgg tttcactcta tacgaaagaa actcgctcat
     1141 atcctgagca tactccagtc tctttatgtt cagctcgtcc ttgacaagat cttcaaccaa
     1201 cctgagatct tttttctcct ctggagtggc acctacaact actttcaaca gaggctgcct
     1261 gttcttgata ttcacctcgt tgcgagccgc cctgcccaag aacacaacct gcctggccag
     1321 atccatctgt ctttccagat cgacatcgcg cgcccattcg tctgctttag gatagtcctg
     1381 catgtgtacg cttcctgagc caggttctag attggtgaat atttcctccg ccaagaaagg
     1441 cgtgaacggc gcaatcagct tggcgatggt tgtcagagtc tcgtggagcg tgagatatgc
     1501 ggcttgcttg tcgacggtca tctcgcttgc ccaatatcga cgacgacatc tgcgcacata
     1561 ccaattggat agatcgtcca cgaactgctc gattcgcctc gcggccgctg tgatgtcata
     1621 gcagtccatc gcctctgtaa cttcgcccac aagcccggat agcttcgaca ggagccacct
     1681 atctatcaca gagcgatcct cgggggcaac atggtattgg ctaggatcaa attcatcgat
     1741 gttcgcgtat agcacgtaga acgcgtatac gttccatagc gtccccatga atttgcgttt
     1801 ggcctcgata actccatcct cgtagaatct cgtcgggttc catggcgcgt tcactgtgta
     1861 cagataccac cttacggcat ctgctccatg atcatcaaag agcttccata catccaccac
     1921 attgcctttg ctcttgctca tcttgagccc tgcctcatcc agaacaagcc cgagcacgac
     1981 gcagttctcg aagggaggcc tgtcaaacag gagcgtggag atggacaaga gcgtgtagaa
     2041 ccatccgcgg gtctggtcca ccgcttcggt tatgaaatcc gccggaaagg ctgcagagaa
     2101 agcttgagca ttttcaaacg gatagtgcca ttgcgcaaac ggcatagagc cagaatcaaa
     2161 ccaacagtcc attacctcag gcacacgttt ggaatctcct ccacactcgg ggcatgccaa
     2221 aactgcctgg tcgatgtacg gtctgtgcag ctctaaacgg tcgggcaacg atttcgccat
     2281 gctcttcagc tcttcaacac tgccaacggc gtgttcatgg ccacactcct cacataccca
     2341 gatcggcaac ggcgttcccc aatagcgctc acggctgacc gcccaatcaa tggcgttttc
     2401 aaggaagttg ccgaatctgc catctcgtat atactcggga taccaactga tcttggcgtt
     2461 attagccaat agctgctcgc gcaggtcaga catgcgtata aaccaagagc cccgcgcgta
     2521 atagagcagc ggcgtatcac atcgccagca ataggggtag ctatgtgtat agtccatctc
     2581 ttcaaacaaa aggccgcgcg ccttaagatc ttgcacaatc agaggatccg catcttttac
     2641 gaacatcccg ctccaatcac gagccctgtc atcaaatctg ccttgggaat ccactagctg
     2701 tattatgggc aagccattgt tctgtgcaac gcgcatgtcg tcttcgccga acgcaggtgc
     2761 tatgtggaca attcccgtgc cctctgaaag agtgacgaag tcgccctcta caaccatgta
     2821 ggcaggcgct tcaggattct catacgtata aagaggcacg tattccatgc ccataagctc
     2881 ggaccccttg accacccgca cgattctgta atcgcctttg accctcgact cgaccaaatc
     2941 ctttgcgagt atgagcgttt ccccatccct ctcgacatag gcataatcat agtcagccga
     3001 cacagccaat gccacgttag acggcaaagt ccacggcgta gtcgtccatg ccagaagcga
     3061 tacattctcc tcgcctttga ccttgaactt cacgaaaatg gatgtctctt caacctcttt
     3121 gtatccttgc gccacttcat ggctggatag cgcagtgccg catcggggac aataggggac
     3181 caccttgtat ccgtggtaca tgaggccctt gtcccagata gtcttcacag cccaccagac
     3241 agattcgatg tactgatcat ggaaagtcac ataagggtcg tccatgtcta tccaaaagcc
     3301 tagcctttca gtcatgcgac gccattcact ttcgtatttg aagaccgact ctttgcaccg
     3361 ctggacgaac tcctctaccc catactcctc aatctggggc ttgccactta tgcctaattc
     3421 cttctcgact tcgagctcaa caggcaaacc atgagtatcc cagcctgcct tgcgtggcac
     3481 gtagaagcct ttcatggttt tgtagcgaag tacgacatct ttcatggcgc gcgtaagaac
     3541 atgtcctgga tggggcagac catttgctgt tgggggcccc tcgtagaaga cgaaccgagg
     3601 gcctttttcc ctccggtcga cgctcttctc gaatatcttt tcttcccgcc aaacctccag
     3661 tatctcttct tccattcgag ggaagtccat agccgaatcg actttcttga acattcgcag
     3721 acataacctc cttgtcttct gtgtcacgga catgtagcag cccaaaacag aaaagataaa
     3781 gccccccgtc caagggacga ggggttaact cgcggtacca ccctaaatta gccgacacat
     3841 ggcctggttt caccaggcgg caacggaacc gtcgcagaaa tgtcggccct ctcgttagcc
     3901 ccgataacgc agggatgcgg cacagcttac tcgttccctt tcagcctgca actcgggggc
     3961 gattttcccg acccgaccag catggagatc ccaccatcct ccactctctt tggccgtccc
     4021 gatcgatact cttccccgtc atcgtatttc gcctattcaa ctaggatcaa tctaccacac
     4081 agctgcatac agcgtcaact catggccgat tcttgacggg aaaacagggt tgccccgtct
     4141 gcgaccctcc ctgtctcctc aaaccttcca tggtcaccct tggctccggt tcccattgcc
     4201 ccttccgcca ttctctggac gcaagacgcc tttttgcatc aggataacca caaccactat
     4261 tgtaattatc atcttgggca aatagtaagt agcattgtat gtcaacgaat agagccaagc
     4321 aggcgttcct gctggcgcat actgtccaaa aaagatgtgc ccggagagga catggcacat
     4381 caacccgacc agcgttcctc ctatagcccc agtcaaattt cgccaccagc cacgcccaaa
     4441 cgaaaaccaa ggagcaaggc caagagcggt cgaagccaga atatagtcaa gaatagcctg
     4501 catgggatgg atgatttcag atgtagctgt cacaaggtca agcacgcctg ctattaggcc
     4561 cgagagcaag cctggataga ctcccctcga aaaagccatg tagagtatgg ggaccatttc
     4621 cagggagata gaccccccct ggggcatctt gaacagcctt atcatcccca aaacccacga
     4681 aagcgcggca gccatgccta cctccgtcag gactattgta ggagtaggct tgcgtgaacg
     4741 agttgttgac ataacactga tcctccttgt gcgccgcgcc gcggggcact aaacagaaaa
     4801 accgcggtca ccataaatga tccgcagtca catagagact taaccgcttc cctacgctgg
     4861 cattacccag ggcgggagag aattcccccg ctcgatcagg ttccagggta tcgggggtgc
     4921 gtcacaaaac aaggcaagac ttcgtctacc atcaatgcaa gcctgagctc gtgcaacaca
     4981 ccatcccgct ctcagcctgt tggctcccct agcggatcgg cacagtatta agtttgaaca
     5041 tagccatata tcttattcat ttgacgcgcg aacgcgaaat cctcctgtgc ccttcaaatg
     5101 ctgaaagcca cgtgcaagct atcgccgtca tgcccaacta gaatctctcg tatcaggtgt
     5161 tcaggaatta ccgttcccac tgccttccta gctcttcgga tcacttcgct ctccgacatc
     5221 gttccaatct catcagcatc gccctcgtcc atcaccaaag tggccacgag tacacccttg
     5281 atattagtga cctccataca gattgcctcc tcccatgtcg aagttgtgat tcgacgcata
     5341 gaggagccat cctgcggctc acggtcagcg atcttccgat aaaaggccga tataaccaac
     5401 gaatatcaga tcatgatatc gaagatccgt aagatactga tgacaaaact aggatcattt
     5461 cagttccctc gaaagatcac ggcaaaacaa cgatcgtccg taccccgctt ggggccattt
     5521 tgcgaatatc gagtggggtg gcgccaggta gaggcattcc tctcacataa ccgtacgcac
     5581 ggacctctgc ttctcactta catggctgca gtttttccag agccttgcga ggcgtttcca
     5641 gctcaaagcc gatctacccc gcgttggtct tcgtcccttc gcaactgcat cggcttttga
     5701 ctcatgcagc caggctgctg ccatctcagt tttgttctac agacttccgt ccttgcgtcc
     5761 ccggtcaagt ttgtctgcct caacacgaat ccgtccttcc taacttaccc ggccctcggg
     5821 ccttcccgta tcgcgcaagg ttgcctactt taaaggccaa cttcggttcg caatcgctac
     5881 gctccgtgtt cacccttgta acttccttca gaccccacca ctttaggcag cgcccttgtc
     5941 tatggattgt cttcccgcta aaaaggacct ttcgggctca atagatatgc cgggcgaaca
     6001 caaaaacggg atcgcaactc gggcgatccc gcattaccct aatgcacagc ctcttctcct
     6061 attgtcccaa ctgtttggcc aagaatccag ccgctgtttc agcgagtttc aattcgagct
     6121 cgcccatgga cttgccctct tgagacaaca tgatcacagc tccggcgatg tccccttcga
     6181 caattatggg agcaattacc tggctagaca actccaaatc ctcgattggc ggattctcgt
     6241 tggcattgtt ggaaaggaat ggcttcctgt tgcgcatgac ttcctctacg tgctcagtaa
     6301 tccgacgttc atagaaactc ttccttgagg ctcctgctac cgcaacaacc tcatcttgat
     6361 ctgctatgag cactatgtgc ctcgttgctt cgtacagtga atccgcgtat tcttgggcaa
     6421 actctccaag gtccgcaatg ggggaatatt tcttaaggat caccgatccc tcgcgatcgg
     6481 tgaatatctc aagcggatct ccctcgcgta ttcgaagggt cctacggatc tctttgggaa
     6541 cgactactct gccaaggtca tcgattcttc ttactatccc tgtcgccttc acgcacatcc
     6601 ctcccttgtt attattttac cttggcccaa ctcctcttat gctctataac caattccgct
     6661 ttcggtcaaa tctgggtgtg ttcgtgaaga ctcgagcagc attagcagga aagacaggcg
     6721 aaaagcaaaa aggaggctca cgacgatctg ctctccggct gctgcatccc gatttgcgac
     6781 tacccaacac ctttcggcac tgagcagccg gttcacggaa tgcaacaacc tttgatcgac
     6841 caacgtccga acttgggcat catgctgcag tctctcgact gcaacaccac actttcgctc
     6901 gtacgctgat cttcgctcga ccgctgcaga taggtttccc tacaacgacg atctcatcaa
     6961 ccgccgccat aagttggcca acctgcaacg atcttagcgc cggccagttg cagtcctgct
     7021 taaagccgca actgaccttt gctcgacgca caatggaccc gacaccttgc gatatgatca
     7081 gatccattgt gggtcttcgc tcgaccgccg taaacctcgt tagatatcat ggccgattac
     7141 cgtggttata tgcatcagtt tcataacata ttacgacgtg actcggaatc ttttccttgt
     7201 ggtccataag attccctagc gttacagctc atccggaggt aacgtaggac cggcaataca
     7261 cccatggatt gaaccatgcc atcgcctgaa cctcgcatcc cggcctatgc tcaccatttg
     7321 cccgtaaaag cctgtagccg ccccctcgag cggccacagg acagaaacaa aggctagctc
     7381 cacttgccct tggctatatg agcccggatc tcagctttag ccgactgtcc gattatccac
     7441 gcctcttctc gtgcacgctt aaatacagca acacagcctg gatcaagatc atcatctatt
     7501 ccggcttcag ctatctcctc aacctgttcc atgagtgcga tcagcctgca atgaagatca
     7561 ttggtataac gggaccttct gatcagttcc gtctccttat catctacttc ggagaat
//
LOCUS       DAOSAI010000072         7562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6298_length_7562_cov_3.282003, whole genome shotgun sequence.
ACCESSION   DAOSAI010000072 DAOSAI010000000
VERSION     DAOSAI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6298_length_7562_cov_3.282003"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLM83_03850"
     CDS             <1..286
                     /locus_tag="PLM83_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOK70576.1"
                     /translation="PKVVRALAERGGFEVSDIDLAIFTQVNWYSIDIVMADLGLPIEK
                     AHRIMDRMGYTGSACIPMALDDARKQGRINPGDVGSGVGYNQAGVALRMW"
     gene            487..1665
                     /locus_tag="PLM83_03855"
     CDS             487..1665
                     /locus_tag="PLM83_03855"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOK70577.1"
                     /translation="MREVVIASAARTAIGTFGGTLATVPAPRLGTIAVKAAIERAGIR
                     NDAVDEVIMGCVLQAGQGQNPARQAAIGAGLPVEIPAMTVNKVCASGLKSVALAAQAI
                     MVGDAEIVVAGGLENMNMAPYILDKARTGYRMGNAEIIDEMVHDGLWCIFGNTHMGIT
                     AETVAEKYGISREEQDEFAAKSQQKAAEAIEAGRFAEEIVPVQIPQRKGDPIVFAQDE
                     HVRPGTTVEVLAKLRPAFKKDGTVTAGNASGINDGAAAVVVMSAERARDLGIEPLAKI
                     RSYASIGVDPSVMGIGPVPATRKALAKAGLSLEDIDLIEANEAFAAQSIAVGRDLAWD
                     PDKVNVNGGAIALGHPIGASGTRILVTLLHEMKRRDAKLGLATLCIGGGMGMAMIVER
                     "
     gene            1884..2105
                     /locus_tag="PLM83_03860"
     CDS             1884..2105
                     /locus_tag="PLM83_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70578.1"
                     /translation="MTRETVIASFVIRFVKPAVSMGPLERADMDLDDGAPNLRIAVRH
                     IQGGYERRFADLDDAIGFIRDEIQLLELE"
     gene            2118..2987
                     /locus_tag="PLM83_03865"
     CDS             2118..2987
                     /locus_tag="PLM83_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOK70579.1"
                     /translation="MFGQQVVSGLSAGCLYALAALGLVLVYRTMEIVNFAHGEMAMVS
                     TFVAHTLLIKAGLPYILAMIGALIFAFIFGMAVERVFLRPIQEGPLISMMIMTLGLFM
                     VFNGAAGWIWGFDPVSFPYAVKGRPILIGDIIVTRDSLLVLAVTVTIAGVLYAALKFT
                     MAGIAIRAASQNPRAARLMGVPVPKVYSLTWGISAVLGAVAGILIAPTTFLSPSMMAE
                     VQIKAFTAAVLGGFSSLPGAVVGGLLLGVLENLVAGYISTQLKSTFAFALIVIVLLIR
                     PSGIMGATEKRKV"
     gene            2984..4015
                     /locus_tag="PLM83_03870"
     CDS             2984..4015
                     /locus_tag="PLM83_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOK70580.1"
                     /translation="MRLVKDKPKGTRCLLKMPGGRSVLLGAALLLLPLVTMAFGRTYV
                     VYLYSLVMVYVIVSLGLNILTGYTGQISIGHAGFMGIAAYASAIFTGRLGIPFVPAMM
                     LSGLLAAVIGLGLGVPALRLHGHYLAICTMGFGVAVNQLSAVWDGLTGGYQGMKVPEA
                     SIFGYRFESDLSYYYLALAVTWLMVVAARNILRSRPGRALMAVRDSEVAAEAMGINLA
                     KYKVAAFAISAFYAGIAGSLYAHLVKYVSPYDFNMGVSMTLLASICVGGLGSVPGSIL
                     GAIFMTLMPHAFSRVKNLPLVLSGISLILVVLFLPYGLASLPWKIKSARSARSTGGTA
                     GRVETSGDA"
     gene            4002..4781
                     /locus_tag="PLM83_03875"
     CDS             4002..4781
                     /locus_tag="PLM83_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70581.1"
                     /translation="MAMLELEGITVRFGGLVAVDSLSFGVREGTIHGLIGPNGAGKST
                     VFNCISGFVRQDEGSISFRGQRLACEPHDVIARGIARTFQNVELFRGQTVLDNVLIGL
                     HTAGTANFITGAFQTRSARKDEERLREQAMKTLDFLGIGEYAGRIAYGLPYGVQKLVE
                     LARALALRPSLLLLDEPVAGMNPTERGELAEIIRNIKKEFGMTVLLVEHDMSFVMGLC
                     DEITVMNFGRKIAEGTPSEVQQDPQVIQAYLGEDAGDAEAI"
     gene            4765..5508
                     /locus_tag="PLM83_03880"
     CDS             4765..5508
                     /locus_tag="PLM83_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70582.1"
                     /translation="MLRLSDVDVYYGMVGALRNVSMEVRQGEIVALLGANGAGKTTTL
                     RAISGIVRPASGSIEFEGRAIHGLSPERIVSLGIVQAPEGRQVFPELSVKENLLIGTY
                     SRRDRAKIPEDFAHVYDHFPVLKERESQPAGTLSGGEQQMLAIGRALMASPKVLLLDE
                     PSLGLAPLATKQIFNIIEALNAEGTTILLVEQNARLALAVSNRAYILQTGSVALSGPS
                     DELLASEDVKRSYLGGSTGGRDIAEVERA"
     gene            5738..6934
                     /locus_tag="PLM83_03885"
     CDS             5738..6934
                     /locus_tag="PLM83_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK70583.1"
                     /translation="MKRHWTLVLASLMVVLLMGIPGPVTAAPEPGVTANQVLIGAFQD
                     QSGPAAVVGINMRKGMEAYFNWINSKGGVNGRKIRFIVEDDGFQPSRSIAAAKKLIEQ
                     DKVFALVGTLGTPGVQATIDYIQSKKVPSIYQGSGISELAFPPKRYFFPVQPNFISEG
                     RIIAQYVVEGLKCKRIAVACEQTDIGSEGLRGIKEQLAVYKMQPVEVVNFGAADVDFS
                     SQVLKLIKAKPDAVIIYSTLKPCAGFLKQADLMGLDAQFLTTYVNADPIQMPALAGEA
                     CVGLIAPGWVPVLGNDEDSVKYLEIYQATYPNEMPSAFAAAGFIAAEVFCEGLRRCGA
                     NPTREGLVAAMETFRDWNGIMAKGITYTQEWRSGKASMYFMRFAKPKFEEDSIEVISG
                     WVKLKI"
BASE COUNT         1675 a         1911 c         2383 g         1593 t
ORIGIN      
        1 gcccaaagta gtgcgggcgc tcgccgagag gggcgggttt gaagtctccg atatagacct
       61 ggccatattc acccaagtaa actggtattc catagacata gtcatggccg acttgggcct
      121 tcctatcgaa aaggcacatc gcatcatgga cagaatggga tacaccggat ctgcatgcat
      181 tcccatggct ttggacgatg cgaggaaaca ggggaggatt aaccctggcg acgtgggttc
      241 cggagtaggc tacaaccagg ccggagtcgc actgcgcatg tggtagcggg ggagccacga
      301 accccagccg aatcgggggc ttggggcgct gccatggatg gtattgggac tggttcccta
      361 actgagaaag gctgggcggg ggtggaaggt tcaggcccaa cttcgttgaa tcttggaatg
      421 tgcaggggtc tcggcgaatg ctgccttgac cttgtgcgac cagaattgca atggaggtat
      481 agaggcatga gagaggtagt catagcttct gctgcgcgaa ctgccatagg cacgtttggg
      541 ggaactctcg cgactgtgcc cgcgccccgt ttgggcacta ttgcggtaaa ggcggctatc
      601 gagagggcag ggattcgaaa cgacgcggtg gatgaagtga tcatgggttg cgtgctccag
      661 gccggacaag ggcagaaccc tgcgcgacag gcggcgatcg gggccggatt gccagtggaa
      721 attcccgcaa tgaccgtgaa caaagtctgc gcttcagggc ttaagtcggt ggccttagcc
      781 gcccaggcga tcatggtggg tgatgccgag atcgtagtgg cgggcgggct ggagaacatg
      841 aacatggccc catacatcct cgataaggcg aggaccgggt atcgcatggg gaacgctgag
      901 atcattgacg agatggttca tgacgggctg tggtgcatct tcggaaacac ccacatgggc
      961 atcaccgcgg agaccgttgc cgaaaagtac ggaatttccc gcgaagagca ggacgagttt
     1021 gcggccaaga gccagcaaaa agcggccgag gccatagagg ccggcagatt tgctgaagag
     1081 atagttcccg tacagatccc ccagagaaag ggagatccga tcgtatttgc ccaggatgag
     1141 cacgtaaggc caggtacgac agtggaggtt ctcgcaaagc tcaggccggc ctttaagaag
     1201 gacggcacgg taactgctgg aaacgcctcg ggcatcaacg acggcgcggc ggcggtagtg
     1261 gtcatgtcgg ccgagcgcgc acgcgacctg gggatagagc ctctggccaa gatcagatcc
     1321 tacgcctcta taggtgtcga tccgagcgta atggggatcg gaccggtgcc tgccaccaga
     1381 aaggcgcttg caaaggccgg actgtccctg gaagatatcg acctgattga agcgaacgaa
     1441 gcctttgctg cgcagtccat agcggtcgga cgcgacctgg catgggatcc tgacaaggtg
     1501 aacgtcaacg gaggcgctat tgcccttggc catcccatcg gcgcaagcgg aacgaggatc
     1561 ctcgtcaccc tcctccatga gatgaaacgg agagatgcca agctcggtct tgctaccctt
     1621 tgcatcggcg gaggcatggg catggcgatg atagttgaga ggtaatctct acaggcaatc
     1681 actttgccgg gtggtccgaa agttcggagg cttgggaact tccgaggcaa gcctcgtcag
     1741 ctggcgagcg atgcaaagca gaacattggg aatttgagat ggacgcgggt ggggccggat
     1801 gacgaccctg cgcggcaata gcctctggga aggacggtcc cgcatctatt acacttaaca
     1861 gaaatagcgg aggagttcga gaaatgacgc gcgagacagt gattgcgtcg ttcgtgatca
     1921 ggttcgtcaa gcccgcggtg agcatgggcc cgctggagag ggcagatatg gacctggacg
     1981 atggggcgcc aaacctcaga attgcggtca ggcacatcca agggggatat gagcgcaggt
     2041 tcgcggattt ggatgatgcc atcggcttca tccgagatga gatccaactg cttgagctgg
     2101 agtagggggt ggggaatgtg ttcgggcagc aggtagtaag cggcctttca gctggatgcc
     2161 tatatgctct ggcagcgttg gggctcgtac tggtctaccg aaccatggag atcgtcaact
     2221 tcgcccacgg cgaaatggcc atggtatcta ctttcgtggc gcacaccctg ctgattaagg
     2281 caggactgcc atacatactt gccatgattg gggcattgat attcgcattc atcttcggta
     2341 tggcagtcga gcgggtattc ctcaggccga tccaagaagg gcccctcatc agcatgatga
     2401 tcatgacact cgggcttttc atggttttca acggagcggc gggatggata tggggatttg
     2461 acccggtaag ctttccatac gcggttaagg gacgccccat tctcatcggg gacatcatcg
     2521 taacccgcga cagccttctc gtgttggcag tcaccgttac aattgccgga gtgctctatg
     2581 cggcgctcaa gttcaccatg gccggcattg ctataagagc tgcctcacag aacccgcgcg
     2641 ccgcgaggct catgggagtc ccggtgccga aggtctattc gctcacctgg ggcataagcg
     2701 cagttctagg cgctgttgcc ggaatactga tcgctccaac cacgttcttg agccctagca
     2761 tgatggcgga ggtccagata aaggccttca ccgctgcggt gcttggagga ttctcgagcc
     2821 tgcccggggc cgtggtcggc ggattgctct taggcgtgct tgagaacctg gttgcgggtt
     2881 acatctctac acagctcaag agcacttttg cctttgctct tatagtcata gttctcctga
     2941 tccgtcccag cgggatcatg ggcgcgaccg agaaaaggaa ggtgtgaggc tcgtgaaaga
     3001 caagcccaaa ggaactcgtt gcttgctcaa gatgcctggc ggtcgcagtg ttttgctcgg
     3061 cgcggccctc cttctgttgc ccctcgttac aatggctttc ggcaggactt acgtagtata
     3121 tctttacagc cttgtcatgg tctacgtcat tgtctcattg gggctgaaca ttctgacagg
     3181 ctatacagga cagatttcca tcgggcacgc tggtttcatg ggaatagcgg cctacgcttc
     3241 ggcgatattc accggcaggt tgggcatccc gtttgttccc gcaatgatgc tttcggggct
     3301 actggcggcg gttatagggc tgggccttgg ggtgcctgcg ctgcggcttc atgggcacta
     3361 cctcgccata tgcaccatgg gctttggagt cgccgtaaac cagctgtctg cggtctggga
     3421 cgggctgaca ggcggatatc agggaatgaa ggttcctgag gcatcgatct tcggctacag
     3481 gttcgagagc gacctcagct actactactt ggccttggca gtgacttggc tgatggttgt
     3541 tgcagcccgg aatatcctcc gttcgcgccc gggtagagcc ctgatggccg tgcgcgacag
     3601 cgaagtcgcg gcagaggcca tggggataaa ccttgccaaa tacaaagtgg ccgcctttgc
     3661 cataagcgcg ttctatgcag ggattgccgg gagcctctat gcgcaccttg tgaagtacgt
     3721 gagcccgtac gatttcaaca tgggggtttc tatgacattg ctcgcttcga tctgcgttgg
     3781 gggcctgggc agtgtgccag ggtctatcct aggggcgatc tttatgacgc tgatgcccca
     3841 tgctttcagc cgagtgaaaa acctgccgtt ggtcctatca ggaatctccc tgatactggt
     3901 agtgctcttc ctgccctatg ggctggcgag cttgccttgg aagataaaga gcgcaagaag
     3961 tgcaagatcc acagggggta cagcggggag ggtagagacg agtggcgatg cttgaacttg
     4021 aagggataac cgtgaggttc gggggattgg ttgcagtcga ctccctcagt tttggggtgc
     4081 gcgaggggac tatccacgga ctgataggtc ccaacggagc cggaaagagc accgtcttca
     4141 actgcatatc tgggtttgtg aggcaggacg aagggagcat atccttccgc gggcagcggc
     4201 ttgcatgcga gccccatgac gtcatcgcac ggggtatagc ccgaacattc caaaacgtcg
     4261 agcttttccg cggacagacc gtactcgaca atgtcctgat cgggctacac acagcgggaa
     4321 ccgctaattt catcaccggg gcattccaga cccggtcggc gaggaaggat gaagagcgcc
     4381 tccgggagca agcaatgaaa accctcgatt ttttgggaat cggcgaatac gccggcagga
     4441 tcgcctacgg tctcccctat ggagtccaaa agctggtgga gctcgccagg gcattggcgc
     4501 tcaggcccag tctgctcttg ctcgacgagc ctgttgcagg catgaaccca accgagcgag
     4561 gggagctggc agagatcata aggaacatca aaaaagagtt cggcatgacg gtgcttttgg
     4621 tcgaacacga catgtctttc gttatggggc tctgcgacga gataactgtg atgaattttg
     4681 gccggaagat agcggagggc actcccagcg aggtccaaca ggacccgcag gtcatccagg
     4741 cctatctcgg ggaggatgca ggagatgctg aggctatctg acgtcgatgt ttactacggc
     4801 atggtcggag cgctccgaaa tgtgtcgatg gaggtgcgcc agggcgagat cgtggccctt
     4861 ttaggggcga atggtgctgg aaagaccaca acgctgcggg cgatctccgg gatcgttagg
     4921 cctgcttccg ggtcaatcga atttgagggc agagcaatcc acggactttc tccggagcgc
     4981 atagtctccc ttggaatcgt tcaggccccc gaggggcggc aggtctttcc ggagctttcg
     5041 gtaaaggaaa atcttctcat tgggacatat tcccgccggg accgcgctaa gattccagag
     5101 gacttcgctc acgtttacga tcattttccc gtcctgaagg agagggaatc tcagccggca
     5161 gggaccctca gcggagggga gcagcagatg cttgcgatag ggcgagcctt gatggcaagt
     5221 cccaaggtct tgcttctcga cgagccttcg ctgggattgg ccccactggc aacgaagcaa
     5281 attttcaaca taatcgaggc actcaatgcc gaaggcacaa cgatactcct ggtggagcag
     5341 aacgccaggc ttgccctagc ggtatcaaac agagcatata tactgcagac gggcagcgtt
     5401 gccctttcgg ggcccagtga cgagctgctc gcaagcgagg acgtgaagcg ctcctacttg
     5461 gggggaagca ctggaggcag agacattgcc gaggttgagc gagcttgatg tatcgcgaag
     5521 tttcagcgtg tagccgaagt cggcccgggc ttggcgattt ccaggactga gtaggcgagt
     5581 cgcggttccg actcgctggc gaagacaacc gagatcagga tactcgggat tgagcggttt
     5641 gagactgtat caggctgcac cttgccgaca agagcgaaga tatgcgcggt gcggtatggt
     5701 gctgaccata cgtcaaaaag ctgaggaggt tagcaggatg aaaaggcatt ggacgttggt
     5761 gctcgcatcc cttatggttg ttcttcttat gggaatcccg ggaccggtca cggccgcccc
     5821 ggagcccggc gtgaccgcaa atcaggttct cataggcgcg ttccaggatc aatcgggccc
     5881 ggccgctgtg gtcggaataa acatgcgcaa gggcatggaa gcctacttca actggatcaa
     5941 ctcgaagggc ggagtgaatg gacggaagat ccggttcata gttgaagacg acggttttca
     6001 gccgtctagg tcgattgcgg ccgccaagaa actgattgag caggacaagg ttttcgcgct
     6061 cgtcggaacg ctggggactc ctggcgtgca ggcgaccata gactacatcc aatcgaaaaa
     6121 ggttccgtca atataccaag gcagcggtat cagcgaatta gcgttcccgc cgaaaaggta
     6181 tttcttcccg gttcagccca acttcatcag cgagggccgc attatcgccc aatacgtggt
     6241 agaagggctg aagtgcaagc ggatcgcagt ggcatgtgaa cagactgaca ttgggtcaga
     6301 aggccttagg ggcatcaagg agcagttggc cgtctacaag atgcaaccgg tcgaagtcgt
     6361 caatttcggc gcagcagatg tcgacttcag ctcccaggtt ctcaagttga tcaaggccaa
     6421 gccggacgcg gtgataatct attccaccct caaaccgtgc gcaggcttcc tgaaacaggc
     6481 tgatctcatg gggcttgatg cgcagttcct cacgacatac gtgaatgccg atccaattca
     6541 gatgccggcg ctcgccggtg aggcatgtgt ggggctgatc gccccgggtt gggtcccggt
     6601 gcttgggaac gatgaggatt ccgtcaagta cctcgaaatc taccaggcca cctatcccaa
     6661 tgaaatgccg agtgctttcg cggcagccgg attcattgcg gctgaggttt tttgtgaggg
     6721 gttgaggaga tgtggggcca atcccaccag ggagggccta gtggctgcaa tggagacatt
     6781 cagagactgg aatggaatca tggctaaggg cataacctac acccaagaat ggcggtcagg
     6841 caaggcttcg atgtacttca tgaggtttgc caagcccaag ttcgaggagg actcgatcga
     6901 agtcatatca ggctgggtta agctgaagat atgacgccgg ggcacagctt gcgtccggag
     6961 ggtcgaaagc aggcgccgaa aacggcaaca agggctaagc aagtcgtcta aagcgtattg
     7021 gcagcaaggc tcaaacctca atcagggaaa acgggatgat tgcgcaaaga ctatctgaga
     7081 attgctaaaa agtatagtct gaagatacta cggttttttt cgtagtatct tttcttatgc
     7141 tgcgaaacaa tcgcagtgtt gcaaagaaat atcacggggc cacagtatct gctctgaccg
     7201 gctcccaatg acccttccca tcggcctccc gtttctgctg ccttcttatg gagtatgccc
     7261 catcgatctg ggcaggtggc ctttgcgagg ctggcgattc tctgatctcc tggaactgga
     7321 tcctaagtgg tggggggtgc tcgagtcctc tctgagatgt gctcagatga aaggtataac
     7381 gtgtgaaatg ttgaaccaat atgaagcgtc aggttactgc tgaagtagga tgtcatccgc
     7441 gggcgcgggg gctggcctga acgtccgcag cgggttggca tgcgcaacag agcacgacgt
     7501 tgacgcgcct tcgcgacaag acaggtaggg caatcgtata cgttcctatt tcacagagga
     7561 gg
//
LOCUS       DAOSAI010000073         7340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6508_length_7340_cov_3.521620, whole genome shotgun sequence.
ACCESSION   DAOSAI010000073 DAOSAI010000000
VERSION     DAOSAI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6508_length_7340_cov_3.521620"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1061
                     /locus_tag="PLM83_03890"
                     /pseudo
     CDS             1..1061
                     /locus_tag="PLM83_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162180.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1641..3509)
                     /locus_tag="PLM83_03895"
     CDS             complement(1641..3509)
                     /locus_tag="PLM83_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70584.1"
                     /translation="MIAEANVEKNERSGVAGVRQGIRLLGRLDDTLARTSVILGIFEA
                     AVPLVAVYMGKRVIETLSSGTGEVMRVVLWAIFLNLGLTLVSKWLQVQLSYGQAEMWR
                     KKDIAVNNKLMEVDYEVVEDPRIHEKLAWLQQTEKMLDRGVPAQADYLKNMARGTFTL
                     VAGLVMSLGVFSVPLPGTDMPLFLQNTLFLALIIALQLYSLWAGARNARGINEISESV
                     TLPMNMLFSAYWPHWQEYSYGKDTRLYTGKALNNALRQMPAVFDRIYDAFMGKYTSIG
                     VRTVANDILTMGLVYGFVGVKAYYGLITPPDIVLYAGTVTLMMSGLTNLAQNYSKAVR
                     NIPYLTVLGDFLDIESERYQGTLPVEKRDDNDYVFEVKNVSFKYPGSDKYALKDVNLK
                     LSVGEKLAVVGMNGSGKTTLIKLLTRFYDPTEGEITLNGVDIRKFNYDEYLELFSVVF
                     QDLKLFSFDIAQNVAAGISPDEERVTEALHMAGFDERLAEMPEGIHTYLYKNFAESGV
                     EISGGEAQKIAMARALYKDAPFVILDEATAALDPISEFEIYTNFDNMVRGRTAVYISH
                     RLSSCRFCDDIAVFDEGRMVQRGSHDELLRDTGGKYYELWNAQAQYYKQEEIDALL"
     gene            complement(3506..5452)
                     /locus_tag="PLM83_03900"
     CDS             complement(3506..5452)
                     /locus_tag="PLM83_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70585.1"
                     /translation="MEANEAKGALSEKAPKRYSSWQNMGYLLKNIARTDKWYLTFMIL
                     MAPVQIVVIAFEAYIPKAVIGGIGQVTTLQAYLWSIFYPVLALLAAKCLSVILDSQLV
                     KGGANQRALYMSDAYTKSMDMDYQFLASDQGQAMVDKGLTLLDQGDMTLIVKFGQRCS
                     GLWAGLGGIIFFAGLVVRIHPCLLIFIIVSGILQYYYGKHVANFKEKMVGETRGLRAK
                     MRYMRFRAGNYEYAKDMRMYRMADWFSDVDKDLEDEWSSYRREEKKRDYGGFVLTAFM
                     IFLRDGLTYFYLATMFLAGSIGVADFVFLLGITRGISGWLMDIIAEVTAIHKDMAPLN
                     DMREFFEYKDALNRDEGVPVPCRHDIQFKDVWFKYPGSEDQAIKGMNFHIKDGEKLAI
                     VGVNGAGKSTIVGLIIGFLHPDKGEILIGGTNAEKMNIYDRYALFSAVFQDVHVFPDT
                     VGNIVMGNDYEALDFMQNTGLGAASDSRMVSADTDARRKRVEEALEKAGLMETVKALP
                     HGIDTYLNKMSRQGAVDLSGGQNQRLMLARAIYRDAPITVLDEPTAALDPIAESEIYG
                     SYSRMVRGKTAVFISHRLASTRFCDRILFIEDGQIAEEGSHEELMRLGGKYREMFDIQ
                     AYYYQDNLGDESGVAASAAGGETA"
     gene            complement(5715..6494)
                     /locus_tag="PLM83_03905"
     CDS             complement(5715..6494)
                     /locus_tag="PLM83_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOK70586.1"
                     /translation="MVPVISLSYAHLVFIVAALIAFFWWFYKVALTRTVDRRIRNYQK
                     DLLERHFEEVNEVYGKMRGFKHDFHNHVQVMEACLELGKYDEMAAYLSQLSEDLEEID
                     WIIRTGNLMLDSILNVKLTLAEKRGINVNARASVPEALTDRGGTIITDVELCTVIGNL
                     MDNAIEGCLTLDDSEARFIRLLIRPMKRNLYIYVSNSYGGELRRIGDAYATTKEGHDH
                     GFGIRSVDGIVAKYGGSVNRQSEPGVFATEILLPLGDTGPS"
     gene            complement(6482..>7340)
                     /locus_tag="PLM83_03910"
     CDS             complement(6482..>7340)
                     /locus_tag="PLM83_03910"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOK70587.1"
                     /translation="NSDENTRRRKKPMFPNERICEVHCNVAAKPRTLRIGICEDSIPD
                     MELIRTYLSGWKARVNSGDRPQAVIPAGIELRIFEFPDAEAFYFNYPDNPRFDVLLLD
                     IHMGDMDGFELAKRIRQKDRRIAVVFITGDPDYASKGYDVDAAGYLIKPVRREELYAV
                     MDKCILSLKRGRMELLWETDGELRKIYQEDILYFESAGHMITAVTADASFESRMSWAE
                     LESLISPDMFARTHRSYIIGLGHVDVLGKTEVTLDNGIVLPVSRRLERELREKFVGYY
                     SGGDGVWFP"
BASE COUNT         1730 a         2027 c         1787 g         1796 t
ORIGIN      
        1 cttcttcgtc gctaacccgt tccctctccc cgtcctactc taaagttaca ccatctggaa
       61 tccctcagtt tagaaagggt aagctgaata cgaatctttt cgaccgttac cagcgcagcg
      121 aacaggcctt gattactaca ttggttgaaa tggtagtaaa cggggtgtct acccgcaaga
      181 tccgaaagat aactcaagag ctatgtggcg tggagatctc aaaatcgaca gtttcagaca
      241 tggtgaaaac gctagaccct gtggttcgtg aatggcgtga acgtcctctc gataaagggc
      301 catacccatt tctgatagtt gatgccatag tgttcaagat acgtaaggga ggtagggttt
      361 ggaaccatag tgcgttgatt gctgtaggca tagactctga aggcatgcgt gagattctcg
      421 ggttcatgct tgcagatagt gagaaggaaa actcgtggcg caatttcttc agggccttga
      481 aagcgagggg cctttcgggc gtggatcttg tggtctcgga tgatcacggt gggttggtca
      541 atgctgtggc catagaattt caggggggca tcatggcagc gttgccagac tcattttacc
      601 aaaaacatcc tggatgcgtg ccccaaggcg cttaagaatc agcttcactc ccgactccga
      661 ctcatgtttg aagcgcccga tatgaatacg gccagagaat tgcttaacgg gatcgtgctg
      721 atttcggaga tctggctccc aaggcaattg aaagacttga agagggcttt gacgatgcga
      781 tggccgtaat ggtcctgcca gagtgtctcc gcaaaaggct gcgaacaacc aactgtgcag
      841 aaaggctcaa cgaagagatc cgaaggcggg aacgagtgat aaggatattc ccaaatgatg
      901 actccgctgt gaggttgatt ggcgctgtgc ttatcgaaaa ggatgaggat tggtctactg
      961 gtcgtaggta cctgaacatg gcggagtatt ggcagtggaa gcaggcttgt gaatcgggcc
     1021 aggcagaatc cttgcctgaa gctgccctcc gcgttgcctg aggctcagcc cttcttgtca
     1081 taatccaagc cgctagccgc cgcccactcc ggctcaggct gagaggcaag cagggtttag
     1141 ccatggagga atatcacggc cttgggcata acttgccgcg aggccgagcc ggagtacaat
     1201 actcactcgg cttggataaa aagtgaaaac caagcgaatg tgtccaacgg agcaaggacg
     1261 aggaattgga tcggattcgc tggcaggttc gattacgtat ctgtataggc atgtttcaac
     1321 gacctagaac catttttaca gcatatctcg gacttgatcg aatcaatcca agtccataag
     1381 ctcttgtata atcccaccat ccctcagaat tcatagcaga atcactatcg caatgaaaca
     1441 atgcattgac aatgcctctg actgaaagga tatcgccatc cggcaggaaa agagtatcta
     1501 ccatcagatt gactgcttca cacggggtga agtggtcccc gacttcctca ttgctagctt
     1561 cattgaacag gcagatgagc tcttcaaggc attcaggtca atacctattg caggcaaata
     1621 gcaccacaga gcaggcaagc ctacagcaaa gcgtctatct cctcctgttt gtagtattgg
     1681 gcctgggcgt tccaaagctc gtagtatttg ccgccggtat cccgcagaag ctcgtcgtga
     1741 ctgcccctct gcaccatacg gccctcgtca aacaccgcga tatcgtcgca gaacctgcac
     1801 gacgaaagcc tgtgagagat gtagaccgca gtcctgcccc gcaccatgtt gtcgaagttg
     1861 gtatatatct cgaattcgga tatagggtca agcgccgcgg tggcctcgtc cagtattacg
     1921 aaaggcgcat ctttgtataa agcgcgcgcc atggctatct tctgcgcctc gcctccggag
     1981 atttccacgc cgctttcggc aaaattcttg taaaggtaag tgtgtatccc ttcgggcatc
     2041 tctgccagtc tctcatcgaa ccccgccata tgcaaagcct ctgtcacccg ctcttcgtca
     2101 ggtgatattc cggcggcaac gttctgagct atgtcgaaag aaaacagctt caaatcctgg
     2161 aacaccacgg aaaacagctc tagatattca tcgtagttga actttcggat atctaccccg
     2221 ttgagcgtga tttccccttc cgttgggtcg taaaacctgg tgagcagctt tattagcgtg
     2281 gtcttgccgc tgccgttcat gcccaccacg gccagctttt cgcccacact gagcttgagg
     2341 ttcacgtcct tgagcgcata cttgtcagaa ccaggatact tgaagctcac gttcttgact
     2401 tcaaacacgt aatcgttatc atcccgcttc tctacaggaa gcgtgccttg ataccgctcg
     2461 ctttcgatgt ccagaaaatc gccaagaacc gtcaggtacg gtatgttcct cacagctttg
     2521 ctgtaattct gggcaagatt cgtaagccct gacatcatca aagtcaccgt gccggcgtag
     2581 agcacgatgt ccggcggagt aatgaggccg taatacgcct tgacgccgac gaagccatag
     2641 accaacccca tagtgagtat gtcgtttgcc acggttctta caccaatgct tgtgtactta
     2701 cccataaaag cgtcgtatat ccgatcaaac accgcaggca tctgccgaag agcgttgttc
     2761 agcgctttcc cagtgtagag cctggtgtct ttgccatagg aatattcctg ccagtgcggc
     2821 cagtaagcgc tgaacagcat attcatggga agcgtcaccg actctgatat ctcatttatc
     2881 ccccgcgcat tgcgcgcccc agcccacaga gagtagagct gcaaagcgat tatcaaagca
     2941 agaaacaagg tgttctgcaa gaagaggggc atgtcggtac ccggcagggg aacagagaaa
     3001 actccaaggg acatgacgag tcctgccacc agggtaaaag tccctcttgc catgtttttc
     3061 aggtaatcgg cctgggcggg caccccgcga tccaacattt tctctgtttg ctgcagccat
     3121 gccagctttt cgtggatgcg cggatcctcc accacttcgt agtcgacttc catcagcttg
     3181 ttgttgacgg caatatcctt cttgcgccac atctccgcct gaccgtagct caactgcacc
     3241 tgcagccatt tggatacgag ggtaagcccg agattgagaa agatggccca caacacgacc
     3301 ctcatgactt cgcccgtgcc tgaggaaagc gtttctatga cccttttccc catgtacacc
     3361 gccacaagag gaaccgccgc ctcgaaaatc cccaggatca cggaagttct ggccagcgta
     3421 tcatcgagcc tccccaaaag cctaatgcct tgtcgcactc cggctacacc gctccgttcg
     3481 tttttctcca catttgcctc tgcgatcatg ctgtttcacc tcccgctgcc gatgcggcga
     3541 ctccgctttc gtcaccaagg ttatcctgat agtaataggc ctggatgtcg aacatctctc
     3601 tgtacttgcc gccaagtctc atcaactctt catgactgcc ttcttctgct atctgtccgt
     3661 cctctatgaa cagaattcta tcgcaaaacc tggtggatgc caggcggtgg gatatgaaca
     3721 cggccgtctt gccacgcacc atccggctgt aacttccgta tatttcgctt tcagctattg
     3781 gatctaatgc ggcggtaggt tcgtccaaca ccgttatggg ggcatcacgg taaatggccc
     3841 tggccagcat gagtctttga ttctgtccgc ccgacaggtc cacagcgccc tgtctggaca
     3901 tcttgttgag atacgtgtcg atgccgtgtg gcagcgcctt caccgtttcc atcagccccg
     3961 ccttttccag agcctcctca accctcttac gcctagcatc agtatccgca gaaaccatcc
     4021 tggagtcgga cgcggctcct aaccccgtgt tctgcatgaa atccaatgct tcataatcat
     4081 tgcccatcac gatgttgccc accgtatccg gaaacacgtg cacatcctga aacaccgccg
     4141 aaaacagggc gtaccggtca taaatgttca ttttctcggc gttggttccg ccgatgagta
     4201 tttcgccttt atctgggtga agaaacccaa tgatgagacc gactatagtg ctctttcccg
     4261 cgccgttgac gcccactatg gccagctttt cgccatcttt tatgtgaaaa ttcatgcctt
     4321 ttatcgcctg gtcctcggaa cccggatatt tgaaccacac atctttgaac tgtatatcgt
     4381 gccggcaagg cacgggaacg ccctcgtctc ggttaagcgc gtccttgtac tcgaaaaact
     4441 cgcgcatatc gtttagggga gccatgtctt tgtgaatggc cgtgacctcg gcgattatgt
     4501 ccatgagcca ccctgaaatt cctcgggtta taccaagaag aaacacgaag tctgcaaccc
     4561 caatactgcc tgccaggaac atggttgcca gataaaagta ggtgagaccg tcccttaaga
     4621 agatcatgaa ggctgtgaga acgaatccgc cgtaatcccg ttttttctct tctcttctgt
     4681 atgaactcca ttcatcttct aggtccttgt ccacatcaga aaaccagtcc gccatcctat
     4741 acatgcgcat gtccttggca tattcgtaat tgcctgcgcg aaaacgcatg tacctcatct
     4801 tggccctgag cccccgggtt tcacccacca ttttttcttt gaagttcgcc acgtgtttcc
     4861 cgtaataata ctggaggata ccggaaacta ttatgaaaat caaaaggcag ggatgtatcc
     4921 tcactaccag gcccgcaaaa aagattatgc cgcccagtcc agcccagaga ccggaacaac
     4981 gctgcccgaa cttgacgata agggtcatgt ccccctggtc cagaagcgtc agacctttat
     5041 ctaccatggc ctgcccttgg tcagatgcta agaactgata atccatatcc atgctcttgg
     5101 tgtaagcgtc gctcatatag agggctcgtt gattagcgcc gccctttact aattgagaat
     5161 ccagaatcac ggacaaacat ttggcagcca gaagtgcaag cacggggtaa aatatgctcc
     5221 aaaggtatgc ctgcagtgtc gtcacttgac cgataccacc tataaccgcc ttgggtatgt
     5281 aggcttcaaa cgctatgacg actatctgta caggcgccat cagtatcatg aacgtgagat
     5341 accatttgtc ggtgcgggct atgttcttga gcagatagcc catgttctgc cacgacgaat
     5401 atcttttcgg cgctttttcg gacagtgctc cctttgcctc gtttgcttcc atggtacgca
     5461 cctcctgccg ctgagtgatc tatgaacgcc aaaagcaata tatcatggaa aatgcgagat
     5521 aaatcgtatg tgacatgagc ggtatcatat tcgtcacgaa cggcagaaga cgggacgtgg
     5581 tttacgtgag gcaaggacag tagagccatc actccgtaca accatccaaa gcacctgttt
     5641 gagaagctgc cttcagtaga tatccatgac gggaaagctg tgaactcgta gggcgtcatg
     5701 cgacgcgggg tgaatcatga tgggccagtg tcgcccaagg gcaacagtat ctcggtggcg
     5761 aacacaccgg gttcgctctg gcggttgacc gaaccgccgt atttggcaac tatgccgtcg
     5821 acgcttcgta tgccgaaccc gtggtcgtgg ccttcttttg tggtagcgta ggcatcacct
     5881 atgcggcgga gctccccgcc gtaggagttg ctcacgtata tgtaaaggtt gcgcttcatc
     5941 gggcgtatca agaggcgtat gaagcgcgct tccgaatcat ccagagtaag acagccttct
     6001 atggcgttat ccattaggtt gcctatcaca gtgcaaagct ccacgtctgt aataatcgtc
     6061 ccgcccctgt cggtcaaagc ctcgggcaca gaagccctgg cattcacgtt tatgcccctt
     6121 ttctctgcca gggtaagctt cacattgagt atggaatcca gcatgagatt gccagtcctg
     6181 attatccaat cgatctcttc aagatcttcg gaaagctgag agaggtaggc agccatctcg
     6241 tcatatttcc ccagttcgag gcatgcttcc atgacctgca cgtgattgtg aaaatcgtgc
     6301 ttgaacccgc gcattttgcc gtatacctcg ttcacttcct cgaagtgccg ctccagcagg
     6361 tccttctggt aattgcgtat gcggcgatcc acagtgcgag tcagagccac cttgtagaac
     6421 caccagaaaa atgcgattag ggcggcaacg atgaatacaa ggtgtgcata gctcaaagat
     6481 atcacgggaa ccacacccca tcgcccccgc tgtagtaccc cacgaatttc tcccgcagct
     6541 ctcgttcaag cctgcggctc acaggcaaaa caatcccgtt atccagagta acctcggttt
     6601 tcccgagcac atcaacgtga cccaacccta tgatatacga cctgtgcgtg cgcgcgaaca
     6661 tgtcgggcga gatcaaagat tccagctccg cccagctcat tctgctctcg aaactcgcat
     6721 cggccgtaac cgcagttatc atgtgcccag cagactcaaa atagaggatg tcttcctgat
     6781 agatcttgcg cagctcgccg tctgtctccc atagtagttc cattctgccc ctcttgagcg
     6841 acagaatgca cttgtccatg acggcgtaaa gctcttcccg gcgcacaggc tttatgagat
     6901 accctgcggc gtccacatca taacccttgc tggcgtaatc agggtcgccg gtgatgaaaa
     6961 ccacggcgat cctgcggtct ttttggcgta ttcttttggc cagctcgaag ccgtccatgt
     7021 ctcccatgtg gatgtccaac agcagaacat cgaagcgcgg gttatcgggg taattgaaat
     7081 aaaacgcttc ggcgtcggga aattcaaaga tgcgaagttc aatgccagcg ggaatgaccg
     7141 cttgcgggcg gtcgccgctg ttgactctag ccttccaccc ggaaagatac gtcctaataa
     7201 gttccatgtc gggaattgag tcttcgcata tgcctattct cagtgttctc ggcttcgccg
     7261 ccacgttaca atgcacctcg cagatgcgct cgttcggaaa cataggtttc ttcctgcgcc
     7321 ttgtgttttc gtcgctgtta
//
LOCUS       DAOSAI010000074         7307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6539_length_7307_cov_3.827358, whole genome shotgun sequence.
ACCESSION   DAOSAI010000074 DAOSAI010000000
VERSION     DAOSAI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6539_length_7307_cov_3.827358"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLM83_03915"
     CDS             <1..263
                     /locus_tag="PLM83_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOK70588.1"
                     /translation="LDPTAFAIADISKTHTCRMARSVRQGLRRRGIETGLTVVFSDEP
                     PVVKSSGPIGSISTVPPAAGLAMASVIIRQLASDSGGYGVFQ"
     gene            266..1798
                     /locus_tag="PLM83_03920"
     CDS             266..1798
                     /locus_tag="PLM83_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOK70589.1"
                     /translation="MMKMVGARRRVLTLLIAGLVAALGLSPGAGGAPKQEEAATIQTF
                     AGYGDLLTLSSPPVSGISVPGTIQVSGRSCCSTVLIRCSRQDDPAKFANTFVTVGDDG
                     GFSATVWFFDGPGRYVMDIAAVPRGETVFRVTCRLVVDNTLSTIPLAPRQYSGYGSDL
                     EINSPIASSNRVSDFVTITGKSAFPAVWTSIENAKTGQRRDHIAETLPNGDFAITIPL
                     DVGEGECRISVASRLIAPGTYNTVAEYLVVVDPAPIDMIEPAYRGGTILACDRFLIKG
                     VANADGVRVDVERDGKSAASLKRSRAGAFGLWIDPPAIGEECLVTISSLHRDTNRCVP
                     RRKYRVVRVPKIPLLDDSEKRQIQWDDAEIRMLAESIAPDARDPYDRLRAIHDWIASN
                     LAYDVRAAASGDFGPTDAISVIARGGSVCQGYANVTAALAAAAGLRVRVVGGEGIQEG
                     IRESHAWNEAEVNGRIVLLDTTWDAGHVKDGRFVRMPAAIWFDSSPEEFSLSHSGGLI
                     IP"
     gene            complement(1788..3035)
                     /locus_tag="PLM83_03925"
     CDS             complement(1788..3035)
                     /locus_tag="PLM83_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70590.1"
                     /translation="MSRSRLLTLLCVALICATLIGHGPNTAVAMPSHQIASRGQTLVE
                     RESDSQSILTLFPELKSLPAPLWVQEGLRAYYRTSAATVPNPLSGPNKDAVKPPIMHP
                     EELSPAAPALVRTDVIALATRFSATCSTLFIGDLMIPWEDVTRAAYAGAGDFWINPIV
                     FKDIAKRAPEHLSISEIQFEAGGTKYDSIRFDYRPTGSNKHYVWIFQSSTGLLIYQAQ
                     WLTLSDQRLAYVSAAEFVTKRNISLPWSGEAAPPWAKPGAKLTYQGTIRTWLPTTGHN
                     IPIPALLEVKIEDGGRNWTVAKVTRVFSDDRGQTGAAIKVYSGTGQIGGFWLPKAGLA
                     KLKNGNKLDPFDPLVGSSVEVSYVGKTPSGISVAAIFEWGAKYKRVWIYRSTDGMLLY
                     WLDEKVVDPVTGMVQQAEWQLME"
     gene            3242..4459
                     /locus_tag="PLM83_03930"
     CDS             3242..4459
                     /locus_tag="PLM83_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOK70591.1"
                     /translation="MKLRRTAAVLAAILVTSLLVAGCGPISSTTTGSIAGCVVKHSMA
                     RSLSSPELQDKAVIIVPEPPADSSYVGLPGVRVVAESTGYPSRTYIATTNRYGDFRMS
                     QVEPGEYVVKIADDRFILGYAIQCRVQAGKPTYVGTVPMGSLHLLSVGVSTYRNPSLA
                     PNLTYAASDAGLIAQVLGVDNKLVARTVRLCDYDATKAGILGAIASMGREMVPSDTFI
                     MFFSGHGYQNASTNTEYIVPHDYDGYLTSLIADYELNDYLDRYIPAIHKVFVFDSCYS
                     GGMFKVLDQSMPAGFTRSTGFEIMAKNIVGPGKIVITACDKNEQSIEGPQWNGGLFTW
                     LFAGGMEPYGQVPYPADGCFNHVRDGIITTQEIFDYVEYYVPKEAERVSHTQNPVIYR
                     GPDGNGVWPLFTY"
     gene            complement(4523..5044)
                     /locus_tag="PLM83_03935"
     CDS             complement(4523..5044)
                     /locus_tag="PLM83_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70592.1"
                     /translation="MASSIYGRVGGVIDGLSMNCATDSGSIHGRIGGDIFGADIDLAV
                     DELRGTVSGRIGGVIIGRSVDAKYHAGSNASLCGRVGGIIDGNDISLSGADRITGRCG
                     GTIFGFDCDLKVTGTEMTGRLGGGIIGKSVATRISQDLPPILSALVAVLTYYYYLINS
                     RDSHSGGAGGSGR"
     gene            complement(5090..6292)
                     /locus_tag="PLM83_03940"
     CDS             complement(5090..6292)
                     /locus_tag="PLM83_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70593.1"
                     /translation="MGFLETIGLAFRNLWANRLRTMLSALGIVIGVASVITMISVGTS
                     AERRIASEIAGLGSNMILVTPKIGRGAKSASSPSLTIEMAEELKAAAPAVSAVSPVAQ
                     IEGQLRWASSSTNTSIIGITSDYMRIANQSIVRGRSLIPAEVDSAAKSIILGAKTASA
                     LFEDEDPIGREIGVVAAGRRFDFTVVGVVAAKSDMLFMNSDSQAFVPITCLLYRMMPI
                     RGISMYYCQAQSPEMAGPAVAQVERYLASRTGNPGAFNVGSQEALLDAVHQVTSTMTL
                     LLGIIAGISLLVGGIGIMNTLLTSVAERTREIGIRKSLGARNRDILLQFLVEALALSL
                     IGGAIGMILGWMAGSGISRLAGWPPSFSMTSVAAAVGFSSAVGLIFGIFPAVKAAQMN
                     PVDALRYE"
     gene            complement(6279..6983)
                     /locus_tag="PLM83_03945"
     CDS             complement(6279..6983)
                     /locus_tag="PLM83_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70594.1"
                     /translation="MVDLENVTKIYSMGSFEVTAIQDITLSIADADFVSIMGPSGSGK
                     STLMHVLGCLDRPTSGRYRLCGQDVSRLDDASLAQIRNKRIGFVFQQFNLLPRETILS
                     NVEVPLIYAGVKAKERRRIATEALERVGLSDRMRHRPSEISGGQKQRAAIARALVNNP
                     SLILADEPTGNLDSKTGHEILDILSQLNSEGATVVLVTHDREIAERANRIVHLRDGAI
                     ELVEELRKGGGADGVS"
     gene            complement(7002..>7307)
                     /locus_tag="PLM83_03950"
     CDS             complement(7002..>7307)
                     /locus_tag="PLM83_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK70595.1"
                     /translation="LKPGLTASVKILSRTLSDVVKIPIRSWIDHEGEPSAIRIEDDIP
                     IFVPLELGLSDGEYTHVLSGLEPGDIIVLDAVAVQEKLRRAAGQDRFITFDVQRIDD"
BASE COUNT         1624 a         1934 c         2052 g         1697 t
ORIGIN      
        1 ggcttgatcc tacggcgttc gcaatagccg acatatcgaa gactcacacg tgccgtatgg
       61 ccaggagtgt cagacagggg ttgcgcaggc gcgggatcga aaccggcctc acagtcgtct
      121 tttctgatga accgcccgtt gtcaagagca gtgggccgat aggcagcata tcaacagttc
      181 caccggctgc tggacttgcc atggccagcg tgataatacg ccagcttgca tcggatagtg
      241 gcggctatgg agtgtttcag tgaatatgat gaagatggtt ggcgcgagaa gaagagtttt
      301 gacgttattg atagcagggc ttgttgcagc cttaggattg tctcccggag ccggcggtgc
      361 cccgaagcag gaggaggctg cgacaatcca aaccttcgct ggatacggcg atctgctcac
      421 gctcagctca cctcccgtat ccggcatttc ggttcctgga accatccagg tgagcggaag
      481 gtcatgctgc agcacagtct tgatcaggtg ttcccggcag gatgatccgg cgaagtttgc
      541 gaacactttt gtgactgttg gcgatgatgg cggattctca gcgacagtgt ggtttttcga
      601 tggcccgggt cgatacgtaa tggacatagc cgcggttccc agaggcgaga cggttttcag
      661 ggtaacctgc cgtcttgttg tcgacaatac tttgtctacg attcctttgg cgccgagaca
      721 atattcgggc tacggctcgg atctcgagat caactctccc atcgcatctt ccaacagagt
      781 ctctgatttc gtgacgatca caggcaagag tgcgttccca gcagtgtgga cgagtatcga
      841 gaatgccaaa acggggcaga gacgggatca tatcgccgaa acactgccga acggagattt
      901 cgcgatcacc attcctctgg atgtaggaga gggagagtgc cgaatatcgg tggcgtccag
      961 gttgattgct cctggcactt acaacacggt tgccgagtat ctggtcgtag tcgatcctgc
     1021 gcccatcgac atgattgagc ctgcataccg aggtggaacg atactggctt gcgacagatt
     1081 ccttattaag ggcgttgcca acgctgacgg cgtcagggtg gatgttgagc gtgatgggaa
     1141 aagtgcagcc tctctaaaac ggtccagggc aggcgcattt ggactttgga tagatccgcc
     1201 cgcaattggc gaggagtgcc tggtaaccat atcttcgctt catcgcgaca cgaacaggtg
     1261 tgtgccccgg cgcaagtaca gggtagttcg ggttccgaaa atccctcttc tcgacgattc
     1321 ggagaaaaga cagattcaat gggatgatgc cgaaatcagg atgcttgcgg aatcgatagc
     1381 gccggatgcg cgtgatcctt acgacagact ccgggccata cacgattgga ttgcttccaa
     1441 ccttgcctac gacgtccgcg ctgctgccag cggcgacttt ggaccgactg acgccatttc
     1501 cgtcattgcc cgcgggggaa gtgtgtgcca gggatatgca aatgtcacag ccgctcttgc
     1561 tgctgcggct gggcttcgcg tgcgcgtagt cgggggtgaa gggattcagg aaggaatacg
     1621 agagagccat gcttggaacg aagcggaagt gaatggcagg atagtgttgc tcgatacgac
     1681 ttgggacgcc ggacatgtaa aagacggcag gttcgttcgc atgcctgccg ccatctggtt
     1741 cgattcttct ccggaggaat tcagcttgag ccattcgggc gggctcatta ttccatgagt
     1801 tgccactcgg cttgctgaac catgccggta acggggtcca ccactttttc gtcgagccag
     1861 taaaggagca ttccgtcggt ggaacggtat atccagactc tcttgtattt cgcgccccat
     1921 tcgaatattg ccgccactga aatgccgctc ggggttttgc ccacatagct tacttcgacg
     1981 gaacttccaa cgagcgggtc gaaggggtca agcttgttgc cattcttaag ctttgccaga
     2041 cctgccttgg gtagccagaa tccgcctatt tggcccgttc ccgaatatac tttgatcgcg
     2101 gcgcccgtct gccccctatc atccgagaag actcgggtaa ccttggcaac cgtccagttt
     2161 cggcccccat cttcaatttt aacttcgagc aaggcgggta taggaatgtt atgaccggtt
     2221 gtgggtagcc atgtccttat ggtgccctga taggtcagct tggcgccggg ttttgcccag
     2281 ggaggcgcgg cttctcctga ccaaggaaga gagatgttac gtttcgtcac aaactcggct
     2341 gcggaaacgt aagctaatct ttgatccgag agtgtaagcc attgcgcctg atagatgagg
     2401 agccccgtgc tggactggaa tatccagaca taatgcttgt tgctgcctgt gggcctgtag
     2461 tcgaatctga tggaatcgta ctttgttcca cctgcttcaa attgaatctc ggagatggaa
     2521 aggtgctcgg gagcgcgttt ggcaatatcc ttgaacacta tggggtttat ccagaaatca
     2581 cctgcgcctg cataggcggc ccgtgttacg tcttcccacg gtatcattag gtcgcctatg
     2641 aatagggtgg agcaggtggc cgaaaaccgc gtagcgaggg cgatcacatc agttctgacc
     2701 agagcggggg ctgcagggct gagctcctct ggatgcatga ttggaggctt cacggcgtcc
     2761 ttgttcggcc cactcaatgg gttaggcacc gtcgccgccg atgtccgata gtaagctctt
     2821 agcccttcct gcacccacag gggggcagga aggctcttca actcgggaaa gagggtgaga
     2881 atcgattgag aatcagattc cctttctacc agcgtctgtc ctcggctggc aatctgatga
     2941 gagggcatag ccacagccgt gtttgggcca tgaccaatca gcgtcgcgca aatcagtgcg
     3001 acgcacaata gagtaaggag tcgcgatcgc gacatagcca catcacccca gcatagctca
     3061 aagcaattca ttagaaacaa gttatatatt cctcagctgt gcatcattct ccttcacagc
     3121 gctatatggg caattgcaca gaagatgatt tctaaggaac agattgctgc atatgctcgt
     3181 ccaataggca tacacaatga caacatcgat gttgtgaaac atggggggtg agataagcat
     3241 catgaagctg agaagaacag cggcagttct tgctgcgatt cttgtgacct ctttattggt
     3301 ggccgggtgt ggtccgatat cttcaacaac tacagggtca atcgcgggat gtgtcgtgaa
     3361 acattcaatg gcaaggtccc tttccagccc agagctgcaa gataaagcag taatcatcgt
     3421 gccggagccg cctgcggatt cgtcctatgt tgggctgccg ggcgttaggg tggtagctga
     3481 gagcacagga tacccgtcgc gcacgtatat tgcgacgaca aaccgctatg gcgacttcag
     3541 aatgtcgcag gttgaacccg gagagtacgt agtaaagata gccgatgatc gcttcatctt
     3601 gggatacgca attcaatgtc gggtgcaggc cggaaagccc acatatgtcg gcacggtgcc
     3661 tatgggctcc ctacacctgc tttccgtagg agtaagtacc tacagaaatc cgagcctggc
     3721 gcctaatctg acgtacgcgg cgtccgatgc cgggctcatt gcgcaggtgc ttggcgtcga
     3781 caacaaactg gttgcaagga ccgtgaggct ctgcgattac gacgcgacga aggccgggat
     3841 actcggtgcg atagcatcga tggggcgcga gatggtcccg tcagacactt tcataatgtt
     3901 cttttcgggc cacggatatc agaatgcaag tacgaacacc gaatacatcg ttccgcatga
     3961 ctacgatgga tacctgactt cacttatagc agactatgag ctaaatgatt atctggacag
     4021 atacatccct gcaatacata aggtattcgt attcgattct tgctactcag gcggaatgtt
     4081 caaggttttg gatcaatcga tgcccgcggg attcacccgt tcgacgggct ttgaaatcat
     4141 ggcaaaaaac atcgtgggac cgggcaagat cgtgattaca gcgtgcgaca agaacgagca
     4201 gtcaatcgaa ggaccccaat ggaacggcgg cctgtttaca tggttatttg ctggcgggat
     4261 ggagccgtac ggccaggtgc cgtatccggc ggacggatgt ttcaaccatg ttcgggatgg
     4321 aatcataact acgcaggaga tattcgacta tgtcgagtac tatgtgccga aggaggcaga
     4381 gcgggtatct catacacaga accctgtgat ctatcgtggg cccgacggaa acggcgtatg
     4441 gcctctgttc acgtactgag aatctgagat gagcaatatg gcggcggggt gggcatgcaa
     4501 catgcgataa tcctgccgcc tgttacctcc cgcttccgcc cgcccctccg ctgtggctgt
     4561 ccctgctgtt aatcaggtaa tagtagtaag tcaggactgc cacgagggct gaaagtatag
     4621 gcggcaggtc ttgggatatc cttgtggcta ctgattttcc tatgattccg cctccgaggc
     4681 ggccggtcat ttcagtgcct gttaccttga ggtcgcaatc gaacccgaaa atcgtgccgc
     4741 cgcaccttcc ggttatccgg tcagcgcctg agagagatat gtcgtttcca tcgatgattc
     4801 cgcctacgcg cccgcaaagg cttgcgttgc tcccagcatg atacttcgcg tccacggacc
     4861 ttccgatgat gacgccgcct atgcggcctg agactgttcc cctaagctcg tctactgcaa
     4921 gatctatgtc tgcgccgaat atgtcgcctc cgatgcgtcc atgtattgat ccggagtcag
     4981 ttgcacagtt catactaagt ccgtcgatga ctccgccaac ccttccatag atcgaagagg
     5041 ccatattgta ctcagctccc atgcccaaat atgttctgtc gtaacgcgtt cattcgtatc
     5101 gcagagcgtc aacggggttc atctgcgctg ccttgactgc agggaagatg ccgaagatca
     5161 gccctacagc agaagagaaa cccacagctg ccgccacaga tgtcatgctg aatgagggag
     5221 gccacccggc cagccgtgaa atgcctgacc ctgccatcca gcccaaaatc atgcctatag
     5281 ccccgccgat caggctgagg gccagagctt caacgaggaa ttgaaggagt atgtcacgat
     5341 tgcgggcgcc gagagatttc ctgatcccga tctcccgggt gcgctctgcc actgatgtga
     5401 gcagagtatt cattatccct attccgccta ccaacagact gatgcctgcg ataattccga
     5461 gaagcagagt catcgtggat gttacctggt gcactgcatc tagaagtgct tcctgtgaac
     5521 ctacgttgaa cgcgccgggg ttgccggtgc gggatgccag gtatctttcg acttgggcaa
     5581 ctgccggtcc tgccatctcc ggcgactggg cctggcaata gtacatgctt attccgcgga
     5641 tgggcatcat cctgtacagc aggcaagtga ttgggacgaa tgcctggctg tcggagttca
     5701 tgaacagcat atcgcttttg gcggctacca cgcccaccac cgtgaaatcg aaccgacggc
     5761 ctgctgccac cacgccaatc tcgcggccta tcggatcttc atcttcgaac agagcagagg
     5821 cagtcttggc gccaagaatg atggatttgg ccgcagagtc cacttcagcg gggattagac
     5881 ttcttcccct gacaatactc tggtttgcta tcctcatgta atccgaggtg attccgatga
     5941 ttgaagtgtt tgtgcttgaa cttgcccagc gaagctgccc ttcgatctgc gccacaggag
     6001 atacggcaga caccgcaggc gccgcggcct tcagctcctc agccatctcg attgtgagag
     6061 atggcgaact tgcggacttg gctcctcgcc cgatcttggg ggttacaagg atcatgttcg
     6121 atcccaagcc tgctatctcc gaggcaatgc gccgctcggc gctggttcct actgaaatca
     6181 tggtgataac cgaagcaacg cctattacga tgccaagggc ggagagcatg gtgcgaagac
     6241 ggtttgccca caaattccgg aaggcgaggc ctattgtctc aagaaacccc atccgcaccg
     6301 cctcctttac gaagttcttc gacgagctca attgccccgt cacgcaggtg gactattcga
     6361 ttggcccgtt cagctatctc gcggtcgtgc gttacaagga ctaccgtcgc tccttcggaa
     6421 ttcagctggg atagtatgtc cagtatttca tggccagtct tcgaatccag attccccgtg
     6481 ggctcgtcgg cgagaataag ggaaggattg tttacaagtg ctctcgctat ggccgccctc
     6541 tgcttttgcc cgcctgatat ctcagaaggg cggtgacgca ttcggtcaga aaggcctact
     6601 cgctccaaag cttccgttgc gatccgccgc ctctccttgg ccttcacccc ggcatatatg
     6661 aggggaacct ctacatttga gaggattgtt tcgcgtggaa gcaggttgaa ttgctgaaac
     6721 acgaatccga tccgcttgtt gcggatttgg gccaggcttg catcgtcaag gcgtgacaca
     6781 tcctgcccgc ataggcgata ccggcctgag gtgggccggt cgagacagcc aagcacgtgc
     6841 atgagcgtgg atttgccaga tcctgatggc cccattatgg atacgaagtc ggcatcggca
     6901 atgctgagag tgatatcctg gatagctgtg acctcgaatg aacccataga gtagatcttg
     6961 gtcacgtttt ccagatcaac cacgaaaacc gcctccctgc ttcaatcgtc gatgcgctgg
     7021 acatcaaagg tgatgaaacg atcttgcccc gctgcgcgcc tcagtttttc ctggacggcg
     7081 actgcatcca gtacgatgat atcgccaggc tcgaggccag agagaacatg agtgtattcg
     7141 ccatcagaca acccaagttc gagagggacg aatatgggaa tatcatcttc aatccttatt
     7201 gccgacggtt cgccttcgtg atcgatccaa gagcgaatcg gtatcttgac tacgtcagaa
     7261 agagtgcgcg acagaatctt tacgctcgcc gttagcccgg gcttgag
//
LOCUS       DAOSAI010000075         7220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6634_length_7220_cov_3.659874, whole genome shotgun sequence.
ACCESSION   DAOSAI010000075 DAOSAI010000000
VERSION     DAOSAI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6634_length_7220_cov_3.659874"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PLM83_03955"
     CDS             <1..859
                     /locus_tag="PLM83_03955"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOK70596.1"
                     /translation="LAQPALEQVAQVEDADDADLIGKRVVGEINVSCSECPTCRGGNP
                     RHCEHVKAMGINGWPGCFADYVVLPECNLHVVPDSVSDHQAVFVEPLAAGVEVMEQVH
                     IRPSDEVVVIGDGKLGLLTAQAIHACGNRVTVLGKHVEKLQIARDLGMDVSLVADFVD
                     TVDVVVDCTGNPSGMETAMAMVRPKGTIVLKSTFKQGAPLNLTPIVRNEVTCVGSRCG
                     PFPPALRLLETGAIVTEPLIEAIYPAHQMEAAFERACTRGSLKVLIDFTCSQGSGEVG
                     AGKMERLHF"
     gene            1348..4410
                     /locus_tag="PLM83_03960"
     CDS             1348..4410
                     /locus_tag="PLM83_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HOK70597.1"
                     /translation="MRRLYGVLTLCTLVAMALWLAPASVGSGEPLYVALIWHNHQPFY
                     KNTATDMYMMPWVRMHAVKDYYDMASILKDYPNVHVTFNLVPSLILQLDEYADGAQDV
                     QLLLSLKPASELTDDDKDFILRRFFDANWDNIIKKYPRYWQLLNKRGQGVTDESIAAA
                     IAAYDEQDFRDLQVWFNLAWLDPDFIDEDPSIEALVRKGRGFTEEDKRVVLDKHNEII
                     RNVIPIYREMQNAGQIEVTTTPFYHPIMPLIYDVELARVASPKLELPSEPFAYPEDVE
                     AQLSKAVDFYKDHFAVAPRGLWPSEQAVGQDIVGIVHEAGFQWMVSSEGVLAQSLGIG
                     LRDGAGRIVRPDVLYKPYIVEQDGKEVTILFRDIVLSDKIGFSYSGMSGRAAARDLTD
                     YLRSVKRSLASTPGPHVVTIALDGENCWEYYENDGKEFLHTLYEALNDDPDFKAVTVE
                     EYLAAHPATERIPMLHTGSWISDDLETWIGEVEENRAWDYLSKARTALAGFTAEHADS
                     ADDEVMTALERAWEELYAAEGSDWFWWYGDDQDSGNDEAFDELFRIHLQNIYSYIGLE
                     VPNYLYMPIIPKRAAIPEERITGLMHEPVMDGFIDWGEWEKSSFYPKDDAAVFSWGEG
                     ENIFSSLRVGVDNENLYIRIRTQDGFDELYGKDIKIAAYLTNPRRHEVNTIPRGSASG
                     RDATVLGFAPGTEILVDFAGIERAGRATALLSVAAGDDTWTEVSRINTVGVGEALEIS
                     VPFEVLRMKPADVAHIVVVASSKDQNIDMIPTSGPAEFKAPVVTVGRRLFRVEDPRGD
                     DHGPGTYNYPTNPVFTPGAFDMVSFEVIDSGEEVVFNVELAGEITNPWNSGIGLSVQT
                     IDIYIDTDNKASSGSTKALGGRRVEFEPECAWEYAIWVEGWNQRVFMPEGGDVGGVTA
                     TVDTIGNLVSISVPKSIIGQPEPGWGYQVFVLGQEGYPSEGNLRVREVMEQAAEWRFG
                     GGDNSVYDPNVIDMLVPTGRTQEEILSAYDVSAKKLAVVPMIYPDWEY"
     gene            4425..6629
                     /locus_tag="PLM83_03965"
     CDS             4425..6629
                     /locus_tag="PLM83_03965"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70598.1"
                     /translation="MAAPYVFALKANADRFLISTERTGCTVSERVSFWSAIIVVLALA
                     SPAFAAERIPVAFSFTPPMAANTVAVAGTFNDWNPTANLMTGPDEDGIWTAVIHLQPG
                     RYEYKFVVDGKTWHHDPNAVEQVADPYGGKNSVVYVGVVPVAESSIAFDGWFESKLAK
                     EAGEPFSLSGDAYLKFSGELRPCLETYTELHAWKSLSLGGDAGWHLMPPIDGLEVNQS
                     VATFRLSDGIAAKLFYRGHAGNTKDPLTLIASEGSDNRIWDRRAAEILCSKGIVDGRI
                     GAATLAGNKTLVYGEMEAGLTDRITLGGVATYERWATSAREDGEDHRANIGAYAVAKP
                     TEALTVRGQLLQTHGTKIVSDSTVPVEVEFVYPAERESSGADVIYIVGSFNNWSTEDG
                     LPLAKDADGNWRGSIELLEGEYEYKFWYPDPDNDPNWGHWMGQGTGGDNLVRHVGGDG
                     PSFQDFYFVEPAEHQVYIRGSWDWSKDHELVRDPLTGYWKHTMRLDPGRYEYVFHYND
                     GSQDHWFGQGGDNVRFVAGAGGREVDYSALAYFAEASYQADRYGVTLGLKGAQDGVVA
                     SYGHVGADYRTVYGKAWAQAAEKIKLTLDTSYSTDAAGTKDSATLTVRPGLEILEPAQ
                     HVAFLKGSYEYRKGKSAGGVASLSTRIGSAGLAAEFAHAPLGPSSFKLSVANDFPYDL
                     SAKAEYWKKLKTDDYSLTIEATKKIDWCDGTTIGVSCALPGRKLGAKLSVDF"
     gene            6853..>7220
                     /locus_tag="PLM83_03970"
     CDS             6853..>7220
                     /locus_tag="PLM83_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOK70599.1"
                     /translation="MARFGIHLSISGGLTNATSRAVELGCDCFQIFSRNPRTLKAKPL
                     DPGEANSFRAAVVEAGLKPVVIHANYLINVASPKDETYEKSVSALTDELRRAEILGAD
                     CLVLHPGNHLGSGVEAGSRR"
BASE COUNT         1696 a         1770 c         2138 g         1616 t
ORIGIN      
        1 cctcgcgcag ccagctcttg agcaggtcgc gcaggtagag gatgccgatg atgccgacct
       61 cattggtaag cgtgtggtgg gagagataaa tgtgagctgc agcgagtgcc cgacatgccg
      121 tggcggcaac ccgcgccatt gcgagcatgt aaaagccatg ggcataaacg gttggccggg
      181 ttgcttcgct gactatgtgg tgctgcccga atgcaacctc catgtcgtcc cggactcggt
      241 ctcagaccat caggcggttt ttgtggagcc attggctgca ggggttgaag tgatggagca
      301 ggttcacatc aggccgagcg acgaagttgt ggtgatcggt gatggcaagc tgggcctcct
      361 cacggcccag gccatacatg cctgcggaaa ccgcgttact gtgttgggaa agcacgtgga
      421 gaaactgcag attgcaaggg atctcgggat ggatgtatca ctcgtagccg acttcgtcga
      481 tactgtcgat gtcgtagtcg attgcacggg gaatccatct gggatggaaa ctgctatggc
      541 catggtccgt cccaagggga ccatcgtact taagagcact ttcaagcagg gggccccgct
      601 gaaccttaca ccgatagtcc gcaacgaggt cacttgcgtt ggttcgcgat gcggaccatt
      661 tccgccagcg ctgaggttgc ttgagacggg ggcgatcgtt actgagccgt tgatcgaggc
      721 gatctacccg gctcatcaga tggaggcagc gtttgaaaga gcatgcaccc ggggaagcct
      781 gaaggttttg atcgatttca cgtgctctca aggctcagga gaagttgggg cggggaagat
      841 ggagcgactg catttctagt caggttcgca tcgtgtgatc atgcgcattt cagggagttt
      901 ctgacggctg ttacgttttc acagagccgg tacaggagct ccctaatctc tttggcaagg
      961 gctttatgct cttcctaaat ggtacgctta tcatcaaggc ttccgtaagc ttttgccaag
     1021 aggcagcata cttgctcaaa caggggattc atctgtagca atgttccacc gttttgcagc
     1081 aaaaagttaa gttcctaagc catgaaggaa cggtttgccg agaagttgag ggcaaagctg
     1141 taattcctgt gaaggcttac gtctcggatt gcgaaaggcg gttgcccatt tcgtcgcgtc
     1201 tttggacgtt acagcatcgg taccgacaaa ggatttgcca gatcgatata gaaacctagc
     1261 acgtagtggc acatatgggc tttcgcgcca agtgcggagg tcggaaatgg cggtacacag
     1321 aatccggtat taaaaggaga gatggggatg agaagactat atggcgttct cacactctgc
     1381 acgctggtgg caatggcctt atggctggcg ccagcgtcag tgggctcagg cgaacccctc
     1441 tacgttgcgt tgatatggca taaccaccag ccattctaca aaaacactgc aaccgatatg
     1501 tatatgatgc cttgggtgcg catgcatgca gtaaaagatt actacgacat ggcatcgata
     1561 ctaaaggatt atcccaacgt tcatgtcacc ttcaacctgg tgccgtcact catccttcaa
     1621 ctagatgagt acgcggacgg ggctcaagac gtgcagctcc tgctttctct caaacctgca
     1681 tcggaattga ccgatgatga caaagacttc attctgagaa ggtttttcga tgccaactgg
     1741 gacaacatca tcaaaaagta cccccgatat tggcaacttc tgaacaagcg gggacaggga
     1801 gtgaccgatg aatccattgc cgcggctata gcggcctacg acgaacagga ctttcgcgac
     1861 ctgcaggtct ggttcaactt ggcctggctc gaccctgact tcattgatga agatccgagt
     1921 atcgaggcgt tggtcagaaa gggccgaggc ttcaccgaag aggataagcg agttgttctc
     1981 gacaagcata acgagataat tcgcaacgtc attccgattt atcgggagat gcagaacgca
     2041 ggacagatcg aggttaccac cactccattc taccatccga taatgccgtt gatctatgat
     2101 gtcgagcttg ctcgggttgc tagccccaag ctggagctgc cgagtgaacc gttcgcttac
     2161 cctgaagatg tcgaggcgca gctttcgaag gccgtcgatt tctacaaaga tcactttgct
     2221 gtggctccac gcggactctg gccgtcggaa caggcagttg ggcaggacat agtcggcatc
     2281 gtgcacgagg cgggtttcca gtggatggtt tcgagtgaag gcgtgctcgc gcaatcccta
     2341 ggaataggtt tgcgggacgg ggcaggcagg atcgtccggc ctgatgtcct ctataaacct
     2401 tacatcgtcg agcaagacgg caaagaagtc acgatactct tcagagacat cgttctttcc
     2461 gacaagatag gattctcata ttcagggatg agcggtcgcg cagctgctcg cgatctcacg
     2521 gactatctgc gaagcgtaaa gaggagcctt gcctcaactc ccggcccgca tgtagtcacg
     2581 atcgccctcg atggcgagaa ttgctgggaa tactatgaaa acgacggcaa ggagttcctg
     2641 cacacgctat acgaagcctt gaatgatgat ccggatttca aggcagtcac agttgaggaa
     2701 tatcttgccg cgcatcctgc aacagagaga attcccatgt tgcatactgg ctcatggata
     2761 tctgacgacc ttgagacatg gataggcgag gtggaagaga atcgtgcatg ggattatctg
     2821 tcgaaggccc ggacggctct tgccggcttc acagcagaac acgccgattc ggctgatgat
     2881 gaggtgatga ccgcccttga acgagcatgg gaggagctct acgcggctga gggctctgac
     2941 tggttctggt ggtacggtga tgatcaggat tcgggcaatg atgaagcctt cgacgagctc
     3001 ttcaggatcc acttacaaaa catctactcc tacatcggcc ttgaggttcc aaactatctt
     3061 tacatgccca taatcccgaa gcgtgctgca atcccggaag agcggattac cgggctcatg
     3121 catgaacccg tgatggacgg gttcatcgat tggggcgaat gggagaagtc gtcgttttat
     3181 cccaaagacg atgctgctgt gttcagttgg ggtgaaggcg agaatatctt ctcgtctctg
     3241 cgtgttggag tggataacga gaacctctat atccgcataa gaacgcaaga cggatttgat
     3301 gagctctacg gcaaagacat caagatcgct gcatatctca ccaatccaag gcggcacgag
     3361 gtcaacacta ttccccgcgg gagcgcatca ggacgcgatg ccacggtatt gggattcgct
     3421 ccgggaacag agatactagt ggactttgca ggtatcgaga gagctggccg cgcgaccgcg
     3481 ctcctgtcgg tggcggcagg cgacgatacc tggacagaag tgagtcgtat taacacagtc
     3541 ggagtggggg aggcccttga gatctccgtg ccatttgagg ttttgcggat gaaaccggcc
     3601 gatgtagctc acattgttgt tgtagcatct agtaaggatc aaaacatcga catgatccct
     3661 acatcggggc cggcagagtt caaggccccg gtggtcaccg ttgggcgtcg gctcttcagg
     3721 gtcgaagatc cgcggggtga tgatcatggt cccggaacct acaactatcc caccaacccc
     3781 gtgttcacgc cgggagcctt cgatatggtt tcgtttgaag ttatcgattc cggtgaggag
     3841 gtcgtcttca acgttgagct tgcaggagag ataacaaatc cttggaactc cgggatcggg
     3901 ctttccgtgc agactatcga tatctacatc gatacagaca acaaggcaag ctcgggatcg
     3961 actaaagcgt tgggtggcag aagagtcgag tttgaaccgg aatgtgcgtg ggaatacgcg
     4021 atctgggtcg aaggatggaa ccaaagggtc ttcatgcccg agggtggcga tgttggtggc
     4081 gtcactgcca ccgttgacac cataggcaat ctggtttcga tttcggttcc taagtccatt
     4141 ataggccagc cagagcccgg ttggggctac caagtgttcg tgcttgggca ggaaggttat
     4201 ccatctgagg gcaatctgcg tgtccgcgag gtgatggaac aggccgcaga gtggcgtttc
     4261 ggtggaggcg acaacagcgt gtacgacccg aacgttatcg atatgcttgt tcccacagga
     4321 cgtactcaag aggagatact ttccgcctat gatgtttccg ccaagaagct cgcagtggta
     4381 ccaatgatat accctgactg ggaatactag ctataggggg ccgcgtggcg gccccctatg
     4441 tatttgcgct caaggcaaat gccgacagat ttctgatatc aaccgaaagg acgggttgca
     4501 cagtgtcaga gagagtatcc ttttggtctg caattatcgt ggtcttggca ttagcgtctc
     4561 ctgcttttgc ggcggagagg attcctgttg cattttcatt cactcctcca atggcagcaa
     4621 acacagtggc ggtggcgggc acattcaatg attggaaccc tactgcaaat ctcatgacag
     4681 gccccgacga agatggaatt tggaccgccg taatccatct acagcctgga cgttacgagt
     4741 acaagtttgt agttgatggt aagacgtggc atcatgaccc caatgcggtg gagcaggttg
     4801 cggacccata cggcggcaag aactccgtag tctacgtcgg agtcgttccc gtggccgaat
     4861 cttccattgc ttttgacggg tggttcgagt cgaagctggc gaaggaggca ggagagcctt
     4921 tttcgctctc aggcgacgct tacctcaagt tcagcggaga actccgcccg tgccttgaaa
     4981 cctatactga gctccatgca tggaagtccc tctcgctagg aggcgacgca ggttggcatt
     5041 tgatgccccc cattgacggt cttgaagtga accagtctgt ggccactttc cgcctctccg
     5101 acgggattgc ggccaagctt ttctacagag ggcatgcagg aaacactaag gatcctctca
     5161 cccttatagc ttcagaaggg tcagacaaca ggatatggga ccgtcgtgca gccgagattc
     5221 tctgttctaa ggggattgtt gacggaagga taggcgccgc aacccttgcc ggcaacaaga
     5281 cgttggttta tggggagatg gaagccggcc tgacggatag aatcacattg ggaggagtgg
     5341 ctacatatga gagatgggcc acgagtgcta gggaggatgg ggaagaccac agggccaaca
     5401 tcggagctta cgcagttgca aagcccacag aagccttgac cgttcgcggt cagttgctcc
     5461 agacccacgg cactaagata gtatcagata gcacagtccc tgtggaagtc gagttcgtct
     5521 acccggcaga acgcgaatcg tcaggagctg acgtgatcta catagtcggt tcatttaata
     5581 actggagtac ggaagatggc ttgccccttg ccaaagatgc tgatggcaat tggaggggct
     5641 caatcgaact actcgaaggg gaatacgagt acaagttctg gtaccctgac cctgacaacg
     5701 atccgaattg gggccactgg atgggacagg ggacgggcgg agataatctg gtaaggcatg
     5761 tcggcgggga cggcccgtcc tttcaggatt tctactttgt ggagcctgcc gagcatcaag
     5821 tatacatccg gggatcctgg gattggagca aggaccatga gctcgtgagg gaccctctta
     5881 caggctactg gaaacatacc atgaggctcg atcccgggag atacgagtac gttttccact
     5941 ataacgatgg aagccaggac cattggttcg gacagggcgg cgacaacgtg cgctttgtgg
     6001 ccggagcggg cgggcgtgag gtagactatt ctgcgcttgc ttattttgcg gaagcaagct
     6061 atcaggcgga cagatatgga gttactttgg gccttaaggg tgcacaagac ggcgttgtag
     6121 catcctacgg gcacgtaggc gcagactaca gaaccgtgta tggaaaggcc tgggcacagg
     6181 cggcagagaa gataaaattg acactggata cttcatactc caccgatgcc gcgggaacca
     6241 aggattcggc gactttgacg gtcaggcccg gcttggagat tctagagccg gctcaacatg
     6301 tggccttcct gaagggatca tacgaataca ggaaagggaa gagcgctggc ggtgtcgcgt
     6361 cgctttccac taggataggt tctgcgggtc ttgcagcaga attcgcccat gcccctctgg
     6421 ggcccagcag cttcaagttg agcgtagcca atgatttccc atacgactta tcagcgaaag
     6481 cggagtactg gaagaagctg aagacagacg attacagcct gaccatagaa gcgaccaaga
     6541 agatcgattg gtgtgacgga accactatag gtgtatcttg cgccttgcca ggcaggaaat
     6601 tgggggccaa gttgagtgta gacttctagg ttagcgcatc taagatagtg gcaggaaggg
     6661 tccgtgcccc ataaaggcgc ggacctttgc gttgtccgct catgggcggt gtcccactgc
     6721 acggtcgaag cttgtgccaa acaaagagcg ctttgcttgc caacctgacg tgggacacgc
     6781 tatggaggcg gttgtaagcg cgaggcccta atgtgtagaa tagagacagg cttaccgaag
     6841 ggagataata taatggcccg cttcggcatt catctttcta tttccggcgg tctgaccaat
     6901 gcaacttcga gggcagtgga gctgggttgc gattgcttcc agatcttcag ccgcaatcct
     6961 cgtactttga aggcgaaacc tctcgatccc ggcgaggcca actcatttcg cgcggctgtt
     7021 gtcgaggccg gactgaagcc ggtcgtgatt cacgccaact acttgatcaa cgtggcaagt
     7081 cccaaggatg aaacgtatga aaaatctgtc tcagccctca cagatgagct gcgcagggcg
     7141 gagattttgg gcgctgattg ccttgtcctc catcccggca atcacctggg ttctggagtg
     7201 gaggcggggt ctcggcgaat
//
LOCUS       DAOSAI010000076         7218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6637_length_7218_cov_4.976965, whole genome shotgun sequence.
ACCESSION   DAOSAI010000076 DAOSAI010000000
VERSION     DAOSAI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6637_length_7218_cov_4.976965"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="PLM83_03975"
     CDS             complement(<1..1242)
                     /locus_tag="PLM83_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70600.1"
                     /translation="MFTVGAGLNYIKMPYPVPLAGYQARAELAETQNDPLCARLLTVE
                     GDGGSVAIVVLDVLYVDSDLTDLLRAAVSAQTSIPPSSVMVCSTHTHSGPGLIFPNSN
                     RYDSSIVDSIITAVSEAAGRAWSMRTACELSWASGDIVGVASIRNGVTGEAGPANQAL
                     RVAEFRSVNRNLAGVPRFVARAINFGCHATVLGPGNALVSRDWPGFMVDACEEQLRRA
                     GVQRPFVIFLNGAAADVSARYTRKAQTFEEAARLGRMSADQVFDVFASSGPSRTCHVC
                     LKHESISIPRRQLPSEHCALAAISREEREAHRLASCGGSPLALKEALDRAAAWRIVLN
                     RIRHEHGRPRNPLVAHLCLLRLGDLAMVFVPGEAPAKLGDKIRATVAEGASAPEESVW
                     VVGYSNGHLGYISPMRAGPDGY"
     gene            complement(1257..2147)
                     /locus_tag="PLM83_03980"
     CDS             complement(1257..2147)
                     /locus_tag="PLM83_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOK70601.1"
                     /translation="MSQSQYDDELADAILPRPGCLALIRRMFDSLRPAEKKVASYILD
                     NGGQVVYQSITELASATGTSDATIIRFANALGFSGYQELKIALARELVSPGKNIHDDI
                     EPGDSLPVAVNKAFQANIQAVSDTLNVLDIESLQQAVGAVVGARHVYLYGVGTSALSA
                     QDAYYKLLRVGIHANFYADAHMQAISTALITEDDVVIGFSHSGSTKDVVDVLRLAKER
                     GACIVCITNRARSPMAKLADVVLRTASEETPFGSGGMPSMMAQLSVVDALFVGVSLSM
                     YDRAIEFIERTGETVKDKKY"
     gene            complement(2619..2765)
                     /locus_tag="PLM83_03985"
     CDS             complement(2619..2765)
                     /locus_tag="PLM83_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70602.1"
                     /translation="MSRLLPRLAQIMPWVPMDELPEALASPLVAAGARPGARRSAWPQ
                     ILVI"
     gene            complement(2910..3557)
                     /locus_tag="PLM83_03990"
     CDS             complement(2910..3557)
                     /locus_tag="PLM83_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOK70603.1"
                     /translation="MTKDQIKQEIIRYGNMLIEKGLCTGTGGNISVRMPENNGFMITP
                     SGILYSEIVPDDIVTMDFEGNIIEGSRTPSIERNMHRAIFNARPDVNAIVHTHQNFAS
                     AIAATRQDFPPILDAFVAVFGGGLKCAKYGSIGTEELARNVVEGLGNRNGVLLANHGV
                     LCTGKDLAMAFGNCEFLEASAITYCFANIIGKPVVLDHEIVEKEAADLARRYGQR"
     gene            4704..5036
                     /locus_tag="PLM83_03995"
     CDS             4704..5036
                     /locus_tag="PLM83_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70604.1"
                     /translation="MPVKSLSGALFSIAQPTMVEDFQNGLANRWDPVAVVGGDFGRFA
                     RFEGGKLVVNVPEGNSWRKTGIQSKQPIFTVERSFEAQPGLILIQTDPKLTTGFVVTL
                     ASDRMLLA"
     gene            complement(5464..6342)
                     /locus_tag="PLM83_04000"
     CDS             complement(5464..6342)
                     /locus_tag="PLM83_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOK70605.1"
                     /translation="MAVKILSDSACDLPDEILEEYNIDTLPILVSKGEEEYLDKVTLD
                     PKTMYDEMRKGVVYKTAQIPPQMFSEKFEEIAKNNESVIYVCFSSGLSGTYNTSLIVR
                     DETKRKYPDLDIDIVDSRSASVGFGLLVYKAAKMAKEGKSKEEILKMLDFYVKHIEHI
                     FTVDNLEYLFRGGRVTRAQAFVGGLLNIKPILDIPEDGTLRPIENVRGRKKLLNRMID
                     IMEERGGNADLTHQTIGINHGDDLEGALTLKKMIEERYGCTDFVVNIIGCAIGAHAGP
                     GTLSVFFLNEKPDPEF"
     gene            complement(6624..>7218)
                     /locus_tag="PLM83_04005"
     CDS             complement(6624..>7218)
                     /locus_tag="PLM83_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70606.1"
                     /translation="ILEANYGPRESQAIVEERYSSALAQAVYPDLREFVMAVDEAVWD
                     LMNPDQAVRVPLGIPVFEPRPDEMMAPGPAHDLHDILHSMLVAGASILGVSELDEDGI
                     EIVWTKRLIKGWYAMAYYNLIGEYGEGLIRVNVLLDSPDISRETLEFLLWHEFLHLWL
                     KQGHTREFRRLEMTWPTFIQCKRELDTLGERYGVSFW"
BASE COUNT         1631 a         2055 c         1771 g         1761 t
ORIGIN      
        1 gtacccgtcc ggccccgccc gcatcggcga tatgtatccc agatggccat ttgaatagcc
       61 aaccacccac acactctcct ctggtgcgct ggcaccttca gccaccgtgg cgcggatctt
      121 gtcgcctaac ttggcaggcg cttcacccgg cacgaaaacc atcgccagat cgcccagcct
      181 aagcaagcac aggtgggcaa cgaggggatt acgcggtctg ccatgctcgt gccttatcct
      241 gttgagcacg atcctccagg ctgcggcgcg gtccagtgcc tcttttaggg ccaaagggct
      301 cccgccgcac gaagcgagtc tgtgcgcctc tcgttcctcc cgggagatag ccgctagtgc
      361 gcaatgttcc gaaggaagct gtcgtctggg aatactgatg gattcatgtt tgagacatac
      421 gtggcatgtc ctggagggcc cggaggaggc aaacacgtca aagacctggt ctgctgacat
      481 acgcccaagc cttgcagcct cctcaaaggt ctgggctttt ctggtgtatc tggcgcttac
      541 atcggctgcc gcaccattga ggaaaatcac gaatgggcgc tgcactcctg cgcggcgcaa
      601 ctgttcttcg caggcgtcta ccatgaaacc aggccagtcc cgggagacta atgcgttgcc
      661 cgggcctagc accgtagcat ggcacccaaa gttaatcgct cttgccacga atcttgggac
      721 gcctgcaagg tttcgattga ccgacctaaa ttcagcaacc cgaagagcct gattggccgg
      781 ccctgcctca cctgtgacgc catttcttat gctggcaacg cccacaatat ctcccgatgc
      841 ccagctcaac tcacacgcgg ttctcatgct ccaggcgcgt ccggcagctt ctgatactgc
      901 tgtgattatt gaatccacta tcgaggagtc atacctattg gaattgggga agatcagtcc
      961 cgggccgctg tgtgtgtggg ttgagcagac cattacagat gacgggggaa tggaagtttg
     1021 ggcagatact gcagcgcgca acaagtcagt caaatccgaa tccacgtaca gcacgtcgag
     1081 caccacaatg gctacagatc caccatcgcc ttcaacagta agcaacctgg cacagagagg
     1141 atcattctgt gtctctgcca attcagccct agcctgatac cccgcaagag gcacaggata
     1201 cggcatcttg atgtaattga ggcccgctcc gacagtaaac acctagcgta cccccgtcag
     1261 tatttcttgt ccttaacagt ctcgcccgtt cgctcgataa actcgatggc tctgtcgtac
     1321 attgacagcg atactccgac aaagagcgcg tcaaccactg aaagctgggc catcatagat
     1381 ggcatgccgc ctgacccgaa aggcgtctct tcagaggctg tgcgaagaac tacgtcggca
     1441 agcttagcca tgggcgaacg cgcccgatta gtaatacata cgatgcaggc gcccctctct
     1501 ttggcaagtc tgagcacgtc cacgacgtcc tttgtgcttc cggaatggga aaaccctatt
     1561 acaacgtcgt cttctgttat caaagccgtc gaaatcgctt gcatgtgcgc gtctgcatag
     1621 aagttggcat gtatgcctac ccttagaagc ttgtagtatg catcttgggc tgaaagcgct
     1681 gacgtaccaa cgccatatag atagacgtgc ctcgccccga ctaccgcgcc aactgcctgc
     1741 tgcagtgatt caatgtcgag cacattcagc gtgtcgctta cagcttgaat gttggcctga
     1801 aatgccttgt tgacggccac tggcaatgaa tcgccgggct cgatgtcgtc atggatgttt
     1861 ttgccgggtg atacgagttc tctagccagt gcaatcttga gttcctgata accactgaat
     1921 cccagagcat tggcaaacct gattatcgtg gcgtctgacg tgcctgtggc gctggctagt
     1981 tcagtaatgg actggtatac tacctggccc ccattgtcca agatgtatga ggcgaccttc
     2041 ttctctgccg gccgaagcga atcgaacatt cggcgtatga gagccaaaca acctggcctg
     2101 gggagaatag catcagcaag ttcgtcatca tattggctct ggctcatgta gctcaccccc
     2161 tctgcgctat ttcgtcggac accgtgtatg gagtattgct tcatatgtgt cagtgtctta
     2221 gtggagatat tctgcatatg ttagtggatt ccttccacag aatacgaata atttctgtag
     2281 attatcttca aggtgtgcta gagcgtgagg cagcagagag atggaagggg agcgcgttat
     2341 gacaaagcag ctgctagaga tagctgaacc gagggcttgg cccacatttc gcatgttgcg
     2401 tgttctatat agccatttgg tgcatgtaag gccaaacagc atgtttgaag gccctggact
     2461 atgcataagc atgtctcatg aagttcaagc aagccacaag gagtgtccta gccctatcag
     2521 gcgaccaaag cccaaactgc gggcggcgcg gactcgagga ccgcgcaaga acttgttgaa
     2581 acttgtctag gccaccataa ggttcccatg gagtttgact aaatcacgag tatctgaggc
     2641 catgcgctcc gcctagcgcc cggcctggcg cccgccgcca ccagtgggct cgcaagtgcc
     2701 tcggggagct cgtccattgg cacccacggc attatctggg caagtcttgg caaaagtctt
     2761 gacaagtttc tgcgcgatcc tcggggccgc cgcatcatct gcagtccaaa ccctggccgc
     2821 ctaattggat taagatactt actgcaagtt atttagcgcg gttgtccgcg cgggttacgt
     2881 tacagatgca agtcaccggg tgaatgaacc tatctctgtc cgtagcgtct agcgaggtct
     2941 gccgcctcct tctccactat ctcatgatca agcactacag gcttgccaat aatgttcgca
     3001 aagcaatacg ttattgcgct tgcctccagg aactcgcaat ttccaaatgc catggccagg
     3061 tccttgccgg tacaaagcac accgtggttg gccaagagaa ctccgttcct gttgcccaag
     3121 ccctccacta cattgcgggc aagctcttcc gttccaatgc ttccatactt ggcgcacttg
     3181 aggcctcctc caaaaacggc cacgaaagca tcgaggatcg ggggaaagtc ctgccttgtg
     3241 gccgctattg ctgaagcgaa attctggtgt gtatggacga tggcgttaac atctggccgc
     3301 gcgttgaaga tagccctgtg catgttccgc tcgatgcttg gggttcggct gccttcgatg
     3361 atgtttccct caaagtccat ggtgacaatg tcatcgggga cgatttccga atacaaaatc
     3421 ccgctcggcg tgatcatgaa gccattgttc tccggcatac ggacgctgat gttcccgcct
     3481 gtccccgtac aaagcccctt ctcgatcagc atgttgccgt atctgatgat ttcctgtttg
     3541 atctgatctt tggtcatttc tctggttcct cccgccactt tcattctgta taactcacaa
     3601 tcaactatat ccaatacaga ctcgaaaatc gcccaagagc tcatcgcaac tttccaaggc
     3661 tcctctcccc cacgcccggc agcccagttt ctggaggcag tgcatacgcc cttctgcgca
     3721 ttccctcccc gatctcggca gccctatctt gctcttgcca gggggaaacc aggacccagc
     3781 ttgccacggc gatcgccgtt gtccgccacc tactcctctt cggcgggtcg ccaagctttc
     3841 acggtcacag acaaatcaga gtccggaacg cgtcgatcca tcgctcgatg atgcaactgt
     3901 agatccacat catgacatct acaaggtctc taagtgactt cgggaaaaca gtcaatcgct
     3961 ttcagactaa cacgccttgg gcaccgctcg atatgagtgt gcacatagcg caaggcttag
     4021 ccagcagttg tttcggcgcg tttgcatggt gagtcaaaaa actggcataa cttaatcttt
     4081 ctcctgccta catatgtatc cttttcaatg gacccgcgtt tggattgttt ttttgcaagc
     4141 aagggccagt ttatgcattc agtatggcca taaaaagatg accatcgact tgccgtctga
     4201 tctgcagatc ctcgccccgg atcctgcggc catagccgcc tcgaaggctt atcttctctc
     4261 tgcaaagggg cattccacaa gccctgcccg tactcgccgt tttttctctt ccactacccc
     4321 caacccctcc ccattactca aattcaatct gagcaaaaac gactatccag tgtgagccat
     4381 tacaccattt tctatttggc ttgcaacggt tccacgtgtc gggctatgcc gcgggagaga
     4441 cgggtttgta gaaccatgct ttatcaaaat cggcaagctt atgccgactt gaagagggaa
     4501 tccgcataag gtatcacgac cgagatgcat acaggaagca cgagctgaca aagagctctt
     4561 gttcctagac ccgttgctct tcccgtgggg atttttcatg gccaaaaaag gggattctca
     4621 aatggccatt gacacatagt gttccatgat ggtacctgag taacgtacga tacagacaaa
     4681 acaagttctc cggcacgtcg atagtgcctg tgaagtcact gtccggagct ttgttctcca
     4741 tcgctcagcc gacgatggtt gaggacttcc agaacggttt ggccaatcgg tgggatcctg
     4801 tcgccgtagt agggggcgac tttgggagat tcgcacgatt cgaaggggga aaactcgttg
     4861 tcaacgtgcc cgagggcaat tcgtggcgaa agaccggtat ccaatccaag cagcccattt
     4921 tcaccgtaga gcggtcattt gaagcacaac caggcttgat actgatccag accgatccga
     4981 agcttacgac aggcttcgtg gtcacgctcg cctcagaccg tatgttgctc gcgtgactga
     5041 tgaaggcgaa ggctacctcc cgatcaaacc gattgaagct tccgctgtca tagatatggg
     5101 ctagatcaac gaagggacat cagtgtatct gcaggccttc actcaccccc gcgcaaaatg
     5161 gagaccagcc gccatagtca tcgatcagat tcaggtgggc agataggctg cctagatggc
     5221 aggttcatgt tgagcttcac ctagcttgaa gatcgcaaac tgaccgacag cccttacgaa
     5281 agtgccccat gtggcagaaa tgtacgacgg caggcgtcca ggggccagtt ggatgcgatt
     5341 tcctgcccca actaaagcga gttgcgatag gcgagtcaga taatgagcgc ctccacgagg
     5401 caatccggag gcgcccatta gacagtatcg tttgtatata gatcagtctc tgcccacgtg
     5461 ggctcaaaac tccggatccg gcttttcgtt taagaagaat actgatagtg ttcccggacc
     5521 tgcatgtgcc cctattgcac agcctatgat gttcacgaca aaatcagtgc atccatatct
     5581 ttcctcaatc atctttttga gcgtgagcgc gccttccaag tcatcgccat ggtttatccc
     5641 aatcgtctga tgagtgagat cagcgttccc tccacgctcc tccatgatat cgatcatcct
     5701 gttcaggagc ttctttcttc cccgcacatt ctcgatgggc ctcaacgtgc cgtcttccgg
     5761 tatgtctaag atgggcttta tgtttaacaa cccgccaacg aaagcttggg ccctcgtgac
     5821 ccttcctccc ctgaagaggt attctaagtt gtcgacggtg aatatgtgtt cgatgtgttt
     5881 tacgtagaaa tccaacatct tgagaatttc ctctttagac ttgccttcct tggccatctt
     5941 ggctgccttg taaaccaaga gcccaaagcc cacagaagca gacctgctgt caactatgtc
     6001 aatatccaga tctggatact tgcgtttggt ttcatccctt actatcaatg aggtattata
     6061 agtccctgac aaacctgatg agaagcacac gtatatgacg ctttcattat tcttggctat
     6121 ttcttcgaac ttttcgctga acatctgcgg cgggatctgc gcggtcttgt acactactcc
     6181 ctttctcatc tcgtcataca ttgtcttggg atccaaagtg actttgtcca gatattcttc
     6241 ctcgcccttg ctcacaagta tgggtaaggt gtcaatattg tattcttcca gtatttcatc
     6301 gggcaagtcg caagcactgt cggatagaat cttcacagcc ataaaactcc ccccattctt
     6361 ttagttgatt ataatataca actgccgttc tgtgtgattc ttggcatcat ctggccgtgc
     6421 cagtccaatc ccttctcagg aatgccgcct cagcctgcca ggaacatcgc accataacac
     6481 tgattaccaa catcttgaca catgttcgca cagtcgacaa gatcatgact gcagcttgct
     6541 gcaaaaggcg gcttgagcct cggaacgatg gagtggtctt gtgttcttca ttggctcccg
     6601 ctctccggac atccgatcgc actctaccaa aagctaacgc cgtaacgctc cccgagcgta
     6661 tccaactccc tcttgcactg gatgaacgtg ggccatgtca tctccagtct gcggaattca
     6721 cgggtgtggc cctgtttgag ccagaggtga aggaattcgt gccagagcaa aaactctagg
     6781 gtctcccggg aaatgtccgg gctgtcgagc agcacattta cacggatcaa tccctcgccg
     6841 tattctccta taaggttgta atacgccata gcataccagc cctttatcag cctctttgtc
     6901 cacacaatct cgatcccgtc ctcatcaagc tccgaaaccc ccagtatcga agcgccagcg
     6961 accagcatcg aatggagtat gtcatggagg tcatgtgcgg gaccgggcgc catcatctcg
     7021 tctggtcgcg gttcaaagac agggatacct aggggcactc tgacggcttg atctgggttc
     7081 attaagtccc acacggcctc gtcgactgcc atcacaaact cccgcaggtc agggtaaacg
     7141 gcctgggcca gcgccgagct gtaccgttct tccacgatgg cctgggattc ccgcggccca
     7201 tagttggcct ctaggatc
//
LOCUS       DAOSAI010000077         7207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6649_length_7207_cov_3.651566, whole genome shotgun sequence.
ACCESSION   DAOSAI010000077 DAOSAI010000000
VERSION     DAOSAI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6649_length_7207_cov_3.651566"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLM83_04010"
     CDS             <1..430
                     /locus_tag="PLM83_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HOK70607.1"
                     /translation="SSEFHMGMEYSPETLHGLSHREVGDFTDALAFEIEVPEPFLDRV
                     RGVTDEKLLLEGKDEFVMKAGEHGLLYESIDENGWPIDERVGRTNSTIDMVITMFNEF
                     EPGREIGYVNLPLRDEVVANGLGSYLLDPNSAAPDRVVFD"
     gene            701..2020
                     /locus_tag="PLM83_04015"
     CDS             701..2020
                     /locus_tag="PLM83_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOK70608.1"
                     /translation="MIEIGRHCNSRFWVPPELVEYDALSGDVNLDRTALPLSSEHIVF
                     RRYGALRRAVNALNALPTGSNVLAVVGASSSKAPQVCSMIEGLQSMEEPRVRLLDVRG
                     HADHAIIREGIDIARKTDAALVMAIGGGTTLDVGKAIAGLALQDGGSDIASFQLKKRS
                     INPKQALPWIAVPTTSGTGSESSNNAVVELGEEKRSIRPIPPPFMILADPSLTDSLPF
                     GPTVVSAIDALAQSLEVFTSAGAPMGVQSVALAAFLNLAEGLKALSCATERPESDVRD
                     RLCWGSLLMGIAFAHAHLGLPHALVHHCLRFGLSHGNMVGILLAPGLEIQAAADKETA
                     KRLCKAEQALLALDQDDAFELEGIREGMASNERCTVPDRLIEWLEDLIDQLFTAADLP
                     TSLGSAGLTRCDLDWIASNELESGASFGIPNRPATYGEIMEVLERAF"
     gene            complement(2064..2138)
                     /locus_tag="PLM83_04020"
     tRNA            complement(2064..2138)
                     /locus_tag="PLM83_04020"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2104..2106),aa:Gly,seq:ccc)
     gene            complement(2532..3431)
                     /gene="sdaAA"
                     /locus_tag="PLM83_04025"
     CDS             complement(2532..3431)
                     /gene="sdaAA"
                     /locus_tag="PLM83_04025"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOK70609.1"
                     /translation="MAITTLAELVEEASSRGVSVGAIVREREAEQSGRPEDDLDADMR
                     ERLGVMRSAVDRGLAGVRSRSGLTGGDGPRMASARMNGETICGEPFDSALAYALAVAE
                     VNASMGRIVAAPTAGSCGVLPGVLLSVGERLCVDDLALVEALFASGGVGAVIERSSTL
                     SGAAAGCQAECGSAAAMASAAAVQLAGGSPEACASAVAIAFKGLLGLVCDPVAGLVEV
                     PCIKRNATSVAVALAAADMALAGIRSVIPADEVIEAMGSIGRALPPSLRETALGGLAV
                     TPTGKKVASGISVLGHGNCGLDV"
     gene            complement(3444..4118)
                     /gene="sdaAB"
                     /locus_tag="PLM83_04030"
     CDS             complement(3444..4118)
                     /gene="sdaAB"
                     /locus_tag="PLM83_04030"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HOK70610.1"
                     /translation="MRTVFNIIGPVMVGPSSSHTAGAVRIGLAARAIIGGQPSRAVIT
                     LYGSFATTYGGHGTDLALIAGLLGMSPDDERIPDARRLAAQSGLDVKFEVAEASPDIH
                     PNTAKLELVGPFGATTSVVGSSIGGGSILITKIDHFEVDFSGESHAIVVAYRDQPGII
                     AKITSLLATEDVNIAAMRVSREAKHAKALAIIEVDQPVSDEARALISRIVGVDTAISL
                     PPVSLA"
     gene            4356..5075
                     /locus_tag="PLM83_04035"
     CDS             4356..5075
                     /locus_tag="PLM83_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOK70611.1"
                     /translation="MGNSREGILNGFDLKLIAAALMVLDHVLEYFPSAPIWFGYLGRI
                     VAPIFFYLTVEGFFHTRSRVNYMARLFLGAAVMYVGSRILTHFMPTPVGIPNNIFVSL
                     GLGVALMSAFDWTRETGKYWTGIPMIIAIAAVSLSTEASLYGVAMISVFYFCRNNTKM
                     MAIMYIATSLMISWPSPALNKTHILLYDYQWMMVFATPFFFLYNGKRGPGGEAAKWFF
                     YIFYPAHLWIIYVIQYFTFWV"
     gene            complement(5221..5817)
                     /locus_tag="PLM83_04040"
     CDS             complement(5221..5817)
                     /locus_tag="PLM83_04040"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOK70612.1"
                     /translation="MGQNSRAQYRRIATMGVLTALSIVMLLVLRIPFPPAPFLIYEPA
                     DVALLVAGFALGPVSGLVCTAVACVFQALLLSSDGWFGGLMHLISSGALVAVSAMIYS
                     RNKTKRGAYLALLAGGVAMVAAMIPANIFLIPMFYGMPRQVVVDMIGWIVLFNVLKAG
                     INSVLTALVYKPISGLIKAVPSGSGRSTVKAASKETWR"
     regulatory      complement(5902..6063)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6146..6266
                     /locus_tag="PLM83_04045"
                     /pseudo
     CDS             6146..6266
                     /locus_tag="PLM83_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630198.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6480..>7207
                     /locus_tag="PLM83_04050"
     CDS             6480..>7207
                     /locus_tag="PLM83_04050"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOK70613.1"
                     /translation="MSGDEKQRHRKRWPWIVAILVIAVITVIVSSGKHDRDGVSTRLA
                     AAVAGQETIAVSTGDVHEFVSAAGHLMPIATKTLSFDMPGRITRLKVEEGSRVTAGSV
                     VCELEANSSASLPGDYLRSLQEWVPTPDTGAAEKKLASIRAPFDGVAVSVNASVGSYV
                     TAGQPVITVIDTSSHVVDIEIDEIDLTRVSPGMEATITFDAIPGLKLPGKVDFIAQMA
                     VPKGGIVTIPVRISVDGWHELLKPG"
BASE COUNT         1690 a         2122 c         1882 g         1513 t
ORIGIN      
        1 ttccagcgag ttccacatgg gcatggagta ttctcccgaa acactccatg gattatccca
       61 tagagaagtg ggcgatttca ccgatgctct agcatttgag attgaggttc cagaaccctt
      121 cctcgataga gtgcgcggag tcaccgatga gaagctgctt ctggaaggga aagacgagtt
      181 cgtgatgaaa gccggcgaac acggtctgct ctacgagagc atcgacgaaa acggatggcc
      241 catcgatgag cgcgtcgggc gaaccaactc gactatagac atggttatca ccatgttcaa
      301 cgagttcgaa ccgggcagag aaatcggtta tgtgaacctg ccgctcaggg acgaagtggt
      361 agcaaacgga ttgggcagct acctcctcga ccccaacagt gcggcgccgg acagagtagt
      421 cttcgactga gccgacatag ctcaagtcgt tatgcataat gcaggaacac attacgccac
      481 gcctagcact ttcaaggtgc acggcgtggc gtatccgttc ccgctcgatt cgggttgggc
      541 gaacgcccca taatccatga agacatgcat ccaaatagcc ctggggatgg ttgctcaagg
      601 cttgctaaca tgtgcaacac gaggaaaatc agaccgctgt gtggaacagt agtaccaagc
      661 ataccatgcc ttgcatgatc aagaagttgg tggagaaatt atgatcgaaa tcggcagaca
      721 ctgcaatagc aggttctggg tcccaccgga gcttgtggaa tacgatgcac tgtcaggcga
      781 tgtgaacctt gaccgtacag cgctcccctt atcgtccgaa catattgttt ttcggcgcta
      841 cggcgcgttg cgacgggcag tcaatgccct caacgccttg cctacaggat ccaacgtcct
      901 cgcagtagtc ggcgcgtcaa gttcgaaggc gccccaagtc tgctcaatga tcgaagggct
      961 acaatcgatg gaagaacccc gtgtgcgcct cctcgatgtc cggggccacg ccgaccatgc
     1021 tatcatccgt gagggaatag acattgcccg caagaccgat gccgcactgg ttatggcgat
     1081 cggcggcggg actaccctcg atgtaggcaa ggcgatagca ggtttggcgc tccaagacgg
     1141 aggtagtgat atagcttcct ttcagttgaa aaagaggtca atcaacccca agcaagctct
     1201 cccttggatc gcggtgccca ctacatcagg aactggctcc gaaagctcca ataatgccgt
     1261 tgtggaactg ggcgaagaga agcggagcat ccggcctata ccccctccgt tcatgatact
     1321 tgcggatcct tctctcacag actccctgcc gttcggccct acagttgtgt ccgcaatcga
     1381 cgcgctggca cagtccctgg aggtttttac cagcgccgga gcacccatgg gggtgcaatc
     1441 tgtggccctc gcagcctttc tcaaccttgc ggaaggcctg aaggcgctct cctgcgcaac
     1501 agagagacca gaatcggatg tccgcgaccg actctgttgg gggagcctcc tgatggggat
     1561 agctttcgcc catgcccacc tcggccttcc ccacgcccta gtccaccatt gcctcagatt
     1621 tggcttgagc cacggcaaca tggtgggaat actcctagcc ccggggctcg agattcaagc
     1681 agctgccgac aaagagactg caaaacggct atgcaaagcc gaacaggcgt tgctggcatt
     1741 ggatcaagat gacgcttttg aactggaagg tattagagaa ggcatggcat caaacgagcg
     1801 atgcactgtg cctgatcggt tgattgagtg gctggaggat ctcatcgatc agcttttcac
     1861 cgccgccgat ctgccaactt cacttggaag cgcggggctc accaggtgcg atctggattg
     1921 gattgcctca aacgagcttg aaagcggagc ctcatttggg atacctaacc ggcctgcaac
     1981 ctacggtgaa ataatggagg tgctggaaag agcattctaa gtaagccttg aacatgtgac
     2041 ggaaaagccg cagggaaaat aaatggagcg gatgacggga ttcgaacccg cgactcacgg
     2101 cttgggaagc cgttgcccta ccactgggct acatccgctc gtaatctaca gtatggatct
     2161 tcactacact tattatacgc atcccgagac ggatatgcaa gtgtctgtcc gtgtccggtc
     2221 tccgaattgc tgtcaaatgc tgcccagacc gctccaccac tccctcgggg atctcttaca
     2281 aaggccctca ggaaagaaac aacatcacac gagtcaaatc atgctgcctt caattacgac
     2341 ccggcatcca ctcgcttatc actttgcttc gtacgacgta ttcgctatgt cccattatgg
     2401 tcgaggctaa aactgaaagc gtcggcgcgt agcgcctccg agccccggac gaggctgctt
     2461 gcctacagtt gtgactctcc cggatagcgt ttaacgcgtg aaaccgcgat gccttcgccc
     2521 gaactgcccg tctacacatc aaggccacaa ttcccatgac cgagaacgga gatacccgat
     2581 gcaaccttct tgccggtcgg agtcaccgct aatccgccaa gggctgtttc gcgcagcgac
     2641 ggcggaaggg cgcggccgat cgagcccatt gcttctatga cctcgtctgc tggaataaca
     2701 cttctgattc cggcaagagc catgtccgcg gcagcgaggg caaccgcaac tgatgtcgcg
     2761 ttccgcttga tgcaaggcac ttcaaccagt cccgccacgg ggtcgcagac gaggccgagg
     2821 aggcccttga aggctatcgc aacggcactg gcgcaagcct cgggggagcc tcctgcaagc
     2881 tgcacagcgg ctgctgaggc catggctgca gccgacccgc attcagcctg gcatccggcg
     2941 gccgctcccg aaagcgtact gcttcgctcg atcacagcgc caacgcctcc cgaggcaaat
     3001 agggcctcca cgagagctaa atcatccacg catagcctct ctcccactga gagcaacaca
     3061 cccggaagca ccccgcacga tccggcggtc ggcgcggcga caatccgccc cattgatgcg
     3121 ttgacttccg ccacagccaa agcataagcc agggccgaat cgaatggctc tccacagatc
     3181 gtctccccgt tcattctggc agatgccatg cggggcccgt cccccccagt aaggccgctt
     3241 ctggaccgca ctcccgcaag cccccggtcc acagctgacc tcataactcc aagccgctcc
     3301 ctcatatcgg cgtcaagatc atcttccggg cgtccgctct gctcggcctc tctctcgcgc
     3361 acgattgcgc cgacgctcac gccacggcta gaagcttcct ccactagctc cgcaagggta
     3421 gtaattgcca tgtaaattcc ctcctaggcc aatgacaccg gcggcagcga tattgctgtg
     3481 tccacaccaa cgatgcgcga gatcaacgcc cgtgcctcgt ccgaaaccgg ctggtcaact
     3541 tcgataatcg ccaacgcctt cgcgtgtttt gcttcacgag acacgcgcat ggcagctata
     3601 ttcacatctt cagtagccaa caaagaggtg atcttcgcga ttatgccagg ctgatctctg
     3661 tatgcgacga caatagcatg gctttcaccg gaaaagtcaa cctcgaaatg gtcgatcttg
     3721 gtgatgagga tactacctcc cccgatagat gagcctacta ccgaagtggt cgcgccgaaa
     3781 ggcccaacca gctccagttt ggcggtgttc ggatggatat ccggcgacgc ttccgcgacc
     3841 tcgaatttca catccagtcc gctctgagct gccagacgcc gggcgtccgg aatgcgctca
     3901 tcgtccggac tcatccccag caagcctgca atgagcgcca agtcagtacc gtggcccccg
     3961 tatgtcgtag caaaggagcc gtacagggtt atcacagccc gggaaggttg gccgccgata
     4021 atggcgcggg ctgcaagtcc gattcgaacc gcgcctgcgg tgtgcgaaga agagggccca
     4081 accatcacgg ggccgattat gttaaagaca gttctcatgc gctcgccctc caagttgccg
     4141 acagatgcct ggcccgagca tctttagctc caccgatatc tacatcctcg aaacgattga
     4201 acgcgaggag tgctggtgat aagattgttc ataccaacct cgatcgaacc aaatcttctg
     4261 gagacatatt cccttgtcct cggcccagca tgtgcactgg caagtctgac ttaattatgt
     4321 cagcctatcg cctataagtc gagaggagaa tctccttggg aaatagcaga gaaggcattc
     4381 tgaatggctt cgacctgaaa ctgatcgctg cggccctgat ggtgctcgac cacgtactcg
     4441 agtatttccc tagcgcccca atatggtttg gctatctcgg gcgtatagtt gccccaatat
     4501 tcttctacct gactgttgaa ggcttttttc acaccagaag cagggtcaac tacatggcca
     4561 ggctgtttct gggggcagcg gtgatgtatg ttggctcccg cattctgacg catttcatgc
     4621 cgactccagt ggggatcccg aacaacattt ttgtttcgct tgggcttggc gtcgctctca
     4681 tgagcgcatt cgactggacc cgggagacgg gcaagtactg gacgggaatt ccgatgatta
     4741 ttgcgatcgc tgccgtatcg ctgtcaacag aagcatcttt atatggcgtc gcaatgatct
     4801 cagtattcta cttttgccgc aacaacacca agatgatggc aatcatgtac atcgcaacct
     4861 cgctcatgat ctcgtggcca tccccagctt tgaacaagac gcatatcctg ctgtacgatt
     4921 accaatggat gatggtattt gcaactccat tctttttcct ctacaacggc aaacgaggcc
     4981 ccgggggcga ggcggcaaag tggttctttt acattttcta tccagcacat ctatggataa
     5041 tatatgtgat tcaatatttc acgttctggg tataaagtcg acaacaggct agaagctttt
     5101 tgggccaacc gccgcagatg cagcgcgggc ccaaaaagca ccgattacgt ccccgcctcc
     5161 acaccttccg gtccgttcga ccattcaatc cggaaacagg ttgaacagga cctcagcctg
     5221 tcacctccaa gtctccttgg aggccgcctt caccgtcgac ctcccggatc ctgaaggaac
     5281 cgcctttatc aatcctgaga tgggcttgta tacgagcgcg gtcaaaaccg agttgatccc
     5341 cgctttaagc acgttgaaga gcacaatcca cccgatcatg tccacaacaa cctgtcttgg
     5401 catcccatag aacatcggaa tcaggaagat gttagccggg atcatcgcgg caaccatagc
     5461 caccccgccg gcgagcaatg ccaagtaagc gcctctcttc gtcttattcc tgctgtatat
     5521 catcgcagag actgctacta aggcgccgga cgagataaga tgcatgaggc ccccaaacca
     5581 tccatctgat gagagcagca aagcctgaaa cacgcaggcg actgcggtgc acaccagtcc
     5641 cgacacaggc cccaaagcaa acccggctac gaggagcgcc acatcagccg gttcatagat
     5701 gaggaatggc gcaggcggaa aaggaatcct gagcaccaga agcatcacaa tggaaagagc
     5761 cgtaagcacc cccattgtcg ctatgcgtct gtactgtgcg cgactattct gacccatgac
     5821 gatacccccc cgtggaaaaa tgtatcgaaa aagcccttgg gttgtggccc aggggcttaa
     5881 ggtcgactcg cttttgcaag acgccgcctt ctttcatccg gactcttacc gtcggtcctg
     5941 gattctaacc aggtcagccg cattgcggtt cgtggactcg cctctagtac ctcgcatctt
     6001 ccgccagatg cgccgagaag cttcaccacc ggtcgggaat tacaccctgc cctgaaggcc
     6061 accgtattcg ctttttcaat caatatgata gcacacgccc gatagatagg caattggagt
     6121 tgaccgagcg ttagaatgct gtaggcacct gtcgaacttg aggacggaaa aggccaaagc
     6181 aatccggttt tccacgcttg aggccatcat tggattgccg gccaggcgac atcttaaaac
     6241 actcacctga tgacgaaggg acataggccc tgctcgttgc ggccccgaac aggatgatca
     6301 cccgtatgca gccaaatatc atccctgggg gctataagca ccgctcagaa gtatcctgta
     6361 taataagtac cgaccttcta atacaccttt cactttgcaa gcatacaaat atcggcccaa
     6421 tttggcagga gcctacattc ttgcccaggc aacaagcgag tctcaggagg tgtttacttg
     6481 tgtcgggaga tgaaaagcaa cgccatcgca agcgttggcc ctggatcgtt gctatattag
     6541 tgatcgccgt catcactgtc attgtctctt ctggcaagca cgaccgcgac ggagtgtcga
     6601 cgcgcttggc cgcagccgta gccggccagg aaaccattgc cgtatcaact ggcgatgttc
     6661 atgagttcgt ttcggccgcc ggccacctca tgcccattgc cacaaaaact ctctcatttg
     6721 acatgccggg aaggatcaca agactcaaag ttgaagaagg ttcccgtgta accgcgggat
     6781 cggtcgtatg cgagctggag gccaatagta gcgcctctct gcctggcgac tacttgcgtt
     6841 cactacagga gtgggttccc acgcccgaca ccggcgccgc ggagaagaaa ctcgccagta
     6901 tacgggcacc cttcgacgga gtagcggtgt cagtcaatgc gagcgtggga agctacgtaa
     6961 cggcaggcca acccgtgatc acggtgatcg acacatcctc gcacgtagtc gatatagaga
     7021 tagatgagat agacctcact agggttagcc caggcatgga agcgactata acattcgacg
     7081 ccatccccgg gctgaagctg cccgggaagg tcgatttcat cgcccaaatg gctgttccca
     7141 aaggcggaat cgtgaccatc cctgtaagga tatctgttga tggatggcac gaactgctca
     7201 agcccgg
//
LOCUS       DAOSAI010000078         7167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6703_length_7167_cov_3.240439, whole genome shotgun sequence.
ACCESSION   DAOSAI010000078 DAOSAI010000000
VERSION     DAOSAI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6703_length_7167_cov_3.240439"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLM83_04055"
     CDS             <1..439
                     /locus_tag="PLM83_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HOK70614.1"
                     /translation="SILEIADGRWKLYNGNYSYYREKKTRDEAEAARVAAENAEAALE
                     ARLKASGVAPMRAKARAGASGRDGVGHAQAVVEAIEAEIELKETQVGKVEAELASEEL
                     YVDGERAREAVLIHRQLTEELEALYEAWEKALDEVDVTGGCED"
     gene            436..1926
                     /locus_tag="PLM83_04060"
     CDS             436..1926
                     /locus_tag="PLM83_04060"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOK70615.1"
                     /translation="MTLRVGAYTEACNRAMETARRLGVYAIVRIQDKTERAVGVMNGQ
                     TDLLSIEELSGVGVQIFTFEGYSGFASSDMITLESLEHLVESATNLAKASAKYGAQPS
                     REINQARPLIRDIDVPVPMGLDSISHDEEERLVREVCETAMKIDPRLAVRSFYRIANE
                     QWRVVRSDGTDTMFSMPRSAAMNMITARDGGRAASANASLAGADASIIASSDGRSRLD
                     KRTRKAALTALALLDAPRVAAGPYRVVIDYALAKGLAHEAFGHAAECDGMETSILGLN
                     GKFRKGHRVASDIVNIVDGPVEGDYAYQPISANGIFRATVDIVRRGQLCCGLADIFSA
                     ERAGVPVTGAGRAESFRHVPVPRMTNIRITVENPLPIHKEFEDVEPEDVRDVLARAGL
                     LLDDEPVLYLSGYKGGQVNPAKGDFVFNCSAIYELTHDDIRICQPAIFSGKVLSALGS
                     IIAGIGEPMIDAMGTCGKSGQSVPSSGGGNMFLVIDGNDEITIGGV"
     gene            1928..3238
                     /locus_tag="PLM83_04065"
     CDS             1928..3238
                     /locus_tag="PLM83_04065"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOK70616.1"
                     /translation="MSSVTEIAEALEKTLSQARGWRFVAKSTESVDIGVDDSKLGGAY
                     SPPRSSSEISGNVFIVWEDGRCSRGRVDSKILDRLPRELDLWRSLAYRDDDAAYIWDL
                     ESPADVKLFDPKVAGVVQGDPTPAFEGIGRIVEAARDSGSMVVDARVCCSAGETVVAT
                     SNGLWAKYSETGAWCYWYLDKTLGDGRYGRTTGTWEALDESVEWTARIVDAARIPASS
                     NSGTMRVIMSPSVVDSFIGHYIAFNLSGENVAEGRSRYEVRQFRGGEPALRDDMQVAL
                     DTAVPMGPGSYPCTVEGVASGRADLIKDGRLVTPVLNLKYARRCNMQPTPLPSRGLSP
                     GYAGMLLRTENMGAAESLLEECQDALLVCSVLGMHTQDPVSGNFSLAAPEAIVVKGGE
                     MLGSVKAVISGNFFDALNSRQTRLSLDDQHPNPWMECLCHVEVG"
     gene            3371..4561
                     /locus_tag="PLM83_04070"
     CDS             3371..4561
                     /locus_tag="PLM83_04070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOK70617.1"
                     /translation="MKLSERARAIKPSPTFAVEAKAREMRARGIDVIGFGAGEPDFDT
                     PEHIRDAGIDAINSGFTRYTPTAGIPELKEAIVAKLRRDNGLDYEPSQIIVSCGAKHS
                     LYNAFQALCGPGDEVIIPAPYWVSYPDQVRMSGARPVIVDTSSTDFKITPRVLADAIT
                     PRTRAIILNSPSNPTGVMYSQDELSAIAEIVVENDLFVVSDEIYERLLYRGAEFVSIA
                     ALGEEIKARTLVVNGVSKTYAMTGWRIGYAAGDSELISAMNKIQSHSTSNPCSISQRA
                     ALAAITGPDEPVQYMVEQFRVRGEYMHERLSGMPGIECVEPVGAFYCFPKVSSYYGKT
                     LAGEVVKDSVSFADAILEKAHVAVVAGAGFGTEDYVRMSYATSLEKIKEGLDRLEQAL
                     SEMK"
     gene            4632..5444
                     /gene="mtnP"
                     /locus_tag="PLM83_04075"
     CDS             4632..5444
                     /gene="mtnP"
                     /locus_tag="PLM83_04075"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOK70618.1"
                     /translation="MRIAIIGGTGVYDPRILSNVREETIDTPYGDAGLRIGEYRGEEI
                     AFLARHGFGHTVPPHMINYKANIAALKMLGVERVIATSAVGSLNPDMPPGMFVFFDQF
                     IDFAKSEVRTFFDGGEHGVVHLDYGEPYCPEIRGVLYEKARKLGIEAINGGCYVCTDG
                     PRFETPAEIKMFETLGGDLVGMTSVPETVLAREAQLCYSSIAMITNWAAGISPTPLTH
                     QEVVDIMEANGEKLRSLIMATIEDLPQERGCGCAAAGVDIPWLDEYKKTHWR"
     gene            5468..6733
                     /locus_tag="PLM83_04080"
     CDS             5468..6733
                     /locus_tag="PLM83_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOK70619.1"
                     /translation="MGDSVIRDAGLAPEGRIKIEWARAHMPVVEAIEKEFARGMSLEG
                     LNIVVCCHLEAKTARLAMALAAGGARVAVTGSNPLSTQDDVAAALAQEDGISVYAWHG
                     ATGEEYLQFLDMALDLRPDVLMDDGGDLVGRVHTARRELIPKILGVCEETTTGVHRLC
                     SMEAAGELAFPAVAVNDARMKRLFDNRYGTGQSAWDAIMRTTNLSIAGRCVVVAGYGW
                     CGKGLAMRARGLGAEVIVTEVDPVAACEASMDGFRVMPMERAAGEGQFFVTATGCRDV
                     IRREHLERMRDGAILANAGHFDVEISKPDLASISTGSPTQVRPNIEEYRMPDGRRLYL
                     LAEGRLVNLAAGDGHPVEVMDMSFGVQALSILHIAEHSKELEPKLYSVPAEVDRRVAV
                     LALEAAGICIDSLSEGQRQYMASWQTGTA"
     gene            6836..>7167
                     /locus_tag="PLM83_04085"
     CDS             6836..>7167
                     /locus_tag="PLM83_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK70620.1"
                     /translation="MGALLIEDVTVITMDKYRRIIPKGAVLAESGKITFVGSVDEVPN
                     DLPANLGRISGRGRIAMPGFVNTHTHAAMTLFRGYADDLPLQEWLETKIFPIEARLSA
                     EDVYWGTLL"
BASE COUNT         1621 a         1659 c         2309 g         1578 t
ORIGIN      
        1 ttccatactt gagatagctg atggccgctg gaagttgtat aacggcaact atagctacta
       61 ccgtgagaaa aagacccgag acgaggcaga agccgcccgt gttgctgcag aaaatgccga
      121 ggctgcgctg gaggcgaggt tgaaggcttc gggagtggcg ccaatgcgtg ccaaggcacg
      181 ggcaggcgct tcaggacgtg atggagtcgg tcacgctcag gctgttgtgg aggccattga
      241 ggctgagatc gaactcaagg agacacaggt tggcaaggtc gaagcggagc ttgcttcaga
      301 ggagttgtat gtcgacggcg agcgtgcgcg ggaggcagtg ctgatccatc ggcagctcac
      361 ggaggagctt gaggctttgt acgaggcatg ggaaaaggca ttggacgaag ttgatgtgac
      421 tgggggatgt gaggattgac attgagggtt ggggcctaca ccgaggcatg caacagggca
      481 atggagacgg ccagaaggct tggagtttat gctatcgttc gtattcagga caagactgag
      541 cgtgcagtgg gcgtaatgaa tgggcagact gacctgttga gcatcgagga gctttcaggc
      601 gtgggggtac agatctttac ttttgaaggt tactcggggt ttgcatcgtc cgatatgata
      661 accttagagt ctctcgagca tcttgttgag tctgcaacga atctagccaa ggcgtctgct
      721 aaatacggag ctcagccttc tcgcgagata aatcaggcga ggccgttgat acgcgatatc
      781 gacgtgcccg tccccatggg ccttgacagc atatcccatg atgaagagga acgccttgtc
      841 cgcgaggtgt gcgagacagc aatgaagatc gatccaaggc tggctgttcg ctctttctac
      901 cgcatcgcca atgaacagtg gcgcgtagtc agaagcgatg gcaccgatac aatgttctct
      961 atgccccggt cggctgcgat gaacatgatt accgccagag acggcggacg ggcggcaagc
     1021 gctaacgcct cgctcgcagg ggcggacgca agtattattg catcttcaga tgggcgaagt
     1081 cgacttgata agcggacgcg gaaagcggcg ctcaccgctc ttgcgcttct cgatgcgccg
     1141 cgcgttgcag cgggcccata cagggtagtg atagactacg ctttggcgaa ggggcttgca
     1201 catgaagctt ttgggcatgc ggctgaatgc gatggaatgg agacctcgat tttgggtcta
     1261 aacgggaagt ttcggaaggg gcatcgcgtt gcatcagata ttgtgaacat agtcgatggt
     1321 cccgttgaag gggattacgc gtaccagccg atatcggcga atgggatctt ccgcgccact
     1381 gtcgacattg tccgccgagg ccagctctgt tgcgggctgg cagatatatt ctccgctgag
     1441 cgcgcaggcg ttccagttac gggagcggga agagcggaga gcttccgcca cgtgcccgtg
     1501 cccaggatga ccaatatacg aataacggtg gagaatcctt tgcccattca caaggaattc
     1561 gaagacgtcg agcctgaaga cgtgagagat gttcttgctc gcgcgggcct gctcctagat
     1621 gatgagccag tgctgtatct ttcgggatat aagggaggcc aggtcaaccc ggccaagggt
     1681 gatttcgtct tcaattgttc tgccatctac gagctgacgc atgatgacat tcgcatctgt
     1741 cagcccgcca tattcagcgg caaggtcctc tcggcccttg gctcgatcat tgcaggtatc
     1801 ggcgagccga tgatagatgc gatgggtacg tgcgggaaat cggggcaatc tgtcccctca
     1861 agcgggggcg ggaatatgtt ccttgtaatc gatggaaatg acgagataac gatcggggga
     1921 gtatgaaatg tcctctgtca ctgaaattgc tgaggcgctg gagaagactc tttcacaggc
     1981 gagaggttgg cggttcgtag cgaaatccac ggaatccgtg gacataggtg tcgacgattc
     2041 gaaactgggc ggagcttaca gtcccccgag gtcatcatcg gagatttccg ggaatgtctt
     2101 cattgtatgg gaagatggcc gatgcagtcg cgggagagtc gacagcaaga tcttggaccg
     2161 tcttccgcgt gagttggatc tttggagatc cctcgcctac cgtgatgacg atgctgccta
     2221 catatgggat ctggagtccc ccgctgatgt aaagctattc gatcctaaag tcgctggggt
     2281 ggtccagggt gatcctacgc cggcgtttga aggtatcggt aggatagtag aggctgcccg
     2341 agacagtgga tcaatggtgg tggatgcccg ggtgtgctgt tcggcagggg agacagttgt
     2401 ggcaacatca aacgggctgt gggcgaagta ctctgagaca ggcgcatggt gctactggta
     2461 tctcgacaag actctcggag acggtagata cgggaggacg accgggacgt gggaggcgct
     2521 ggacgaatcg gtggagtgga cggcaaggat cgtggatgcg gccaggatcc ctgcttccag
     2581 caattcagga acgatgcgcg tgatcatgtc gccgagtgtt gtggactcat ttatcggcca
     2641 ttatattgcc ttcaatctga gtggggagaa cgtcgctgag gggagatcca ggtacgaggt
     2701 cagacagttt cgcggtggtg agccggcgct aagagacgat atgcaggtag ctttggatac
     2761 tgctgttcca atgggccctg gctcgtatcc gtgcacagtg gaaggagtcg cctcaggcag
     2821 ggctgatctg atcaaggatg gtaggcttgt tacgccagtg ctcaatctga agtatgctcg
     2881 tagatgcaac atgcagccaa ctccgctgcc atctcggggg ttgtcgccgg gttatgccgg
     2941 gatgttgctg aggacagaga atatgggagc ggccgagagc ttgctcgagg aatgccagga
     3001 cgcgctgctt gtttgcagcg ttctgggcat gcatacccag gaccctgtct caggtaattt
     3061 ttccctggcg gcgccggagg cgatagtcgt gaagggtggt gaaatgctcg ggtctgtgaa
     3121 ggcggtcata tccggcaact tctttgatgc cctcaactca aggcaaactc gtttgagtct
     3181 ggatgatcaa catccaaatc cgtggatgga atgtctgtgc catgttgaag tggggtagac
     3241 aagagggagc tctcacaaga atgttcagtc ctgctttgcg ccaagaggcg atggggagcg
     3301 cgactgcttt cgaagatgtc gaaggaagtc gacgctgatg cataagagta ttcgaagtgg
     3361 agggttgatg atgaagctgt cagaaagggc tagggcgatc aagccgtcgc ccactttcgc
     3421 ggttgaggcg aaggcgcgcg agatgcgtgc tagggggatc gatgtgattg gattcggtgc
     3481 cggggagcct gactttgaca ctccagaaca catccgcgat gccggaattg acgcgattaa
     3541 cagcggattc actcgctaca ctccgacggc ggggattcct gagttaaagg aggccatcgt
     3601 cgcgaaactc cgacgcgaca acggactaga ctatgagcct tcgcagatca tagtctcctg
     3661 tggagccaag catagcctat acaatgcgtt tcaggcgctg tgcgggcctg gggacgaagt
     3721 catcattcct gctccatact gggtgagcta tccggatcag gtgaggatgt cgggggctcg
     3781 gccggtgatc gtcgatacat catccactga tttcaagatc acaccccgtg tgctagcgga
     3841 tgcgattacg cctaggactc gggcaatcat cttgaactcg ccgtcaaacc caacgggggt
     3901 gatgtactca caggatgagc tttcggctat cgccgaaatc gttgtggaga atgatctctt
     3961 tgtggtttcg gacgagatct atgaaaggct actatacagg ggagccgaat ttgtttctat
     4021 agccgctttg ggcgaagaga taaaggcacg gacattggtg gtcaacgggg tttcgaaaac
     4081 ttatgctatg acagggtggc ggataggcta cgctgcggga gattccgagc tcatttccgc
     4141 catgaacaaa atccagagcc acagcacatc caatccttgt tcgatttcgc agagggcagc
     4201 cttggccgcg atcacaggac cggatgagcc tgtccagtac atggtggaac agttccgggt
     4261 gcgcggggag tacatgcacg agagactgag tgggatgccg ggaatcgaat gtgtcgagcc
     4321 tgtcggggcc ttttattgct ttccgaaggt atcaagttat tacggaaaga ctttggccgg
     4381 tgaagttgtg aaggattcgg taagttttgc cgacgccata ctggagaagg cccacgtcgc
     4441 tgtggtggct ggcgcgggtt ttgggacaga ggattacgta aggatgtcgt acgcaacttc
     4501 cctggagaag ataaaggagg ggcttgatag gttagagcag gctctctccg agatgaagta
     4561 ggttctttag catacaggtc gcgcatggat cttcccgaat tggcgtgacc tgtcaggtgg
     4621 aggtggagtt gatgagaata gccataatag gcggaaccgg tgtatacgac cctcgcatac
     4681 tctcaaatgt cagagaggag accattgata ctccttacgg cgatgctggc ctgagaatag
     4741 gggaatatcg gggtgaggaa atcgctttcc tcgcgcggca cggattcggt cataccgttc
     4801 cgccacacat gatcaactac aaggcgaaca tcgcggctct gaagatgctg ggcgttgaaa
     4861 gagtcatcgc cacgtcggcg gtgggctcgc tcaaccctga tatgcctccg ggcatgttcg
     4921 tctttttcga tcagttcatc gattttgcca aatccgaggt gcggactttc tttgatgggg
     4981 gcgaacatgg agtagtccac ctcgattacg gcgagccgta ttgtccggag ataaggggcg
     5041 tactgtacga aaaagcaaga aagctcggga ttgaagccat caatggcggg tgctacgtat
     5101 gcaccgacgg tcctagattt gagactcctg cggagatcaa gatgttcgag actttaggcg
     5161 gcgacctggt gggaatgacg agcgttcccg aaacggtgct ggcccgcgag gcccaactat
     5221 gctactcatc cattgccatg attacaaact gggcagcggg gatctcaccg acccccctta
     5281 cccatcagga agttgtggat atcatggagg ctaacggaga gaagttgcgt tcgctcatca
     5341 tggcaacgat agaagatctg ccgcaagaga ggggatgcgg gtgtgccgca gcgggggtcg
     5401 acatcccatg gctcgacgag tacaagaaga cgcattggag atagcgtggg gggagtgggg
     5461 gtagagcatg ggcgattcag ttatcagaga cgcgggcctc gcgcctgaag gacggatcaa
     5521 gatagagtgg gcgagggctc acatgcctgt tgtagaggcc attgagaagg aatttgcgcg
     5581 tggtatgtca ctagaaggtc tgaatatcgt agtctgctgt catctggagg ctaaaaccgc
     5641 caggcttgcc atggccctcg cggcaggcgg cgctagggtg gccgtgaccg ggagcaaccc
     5701 attgtcaacc caggatgatg tggctgccgc ccttgctcaa gaagacggaa tctcggtata
     5761 tgcctggcat ggggcgaccg gagaagaata cctgcaattc ctcgacatgg ccctcgacct
     5821 ccggcctgat gtactcatgg atgatggcgg tgacttggtg ggcagggttc atacggcaag
     5881 acgagagctg attccgaaga tccttggagt gtgcgaagaa acgacgactg gggtgcaccg
     5941 gctttgctcc atggaggccg ctggagagct tgcctttcct gcagtggctg tgaatgatgc
     6001 gaggatgaag cgactcttcg acaacaggta tgggactggc cagtctgcgt gggatgcgat
     6061 catgcgcacg acgaaccttt ctatagcagg ccgttgtgtg gtggttgcgg ggtatggttg
     6121 gtgcggaaag ggccttgcga tgagggcacg cggccttggc gccgaagtga tagtcaccga
     6181 agtagatcct gtagccgcgt gcgaggcgtc gatggatgga ttcagggtaa tgcccatgga
     6241 acgtgctgcg ggagaaggcc aattcttcgt caccgccaca ggatgccggg acgtaattcg
     6301 aagagagcat cttgagcgga tgcgcgacgg cgctatcctg gccaatgctg gacatttcga
     6361 tgtagaaata tcaaaacccg atcttgcgtc gatttcaaca ggaagcccga cgcaggttcg
     6421 gcccaatata gaggaatatc gcatgcctga tggtcgtcgc ctatatctgc ttgcagaggg
     6481 gcgtctggtt aacctagccg caggggatgg gcaccctgtt gaagttatgg acatgagctt
     6541 cggtgtgcag gccctctcca tattgcatat tgcggaacac agcaaggagc ttgagccgaa
     6601 gctttactct gtgccggctg aggtggatcg acgggtggca gtgcttgcgc tcgaggcagc
     6661 cggtatttgc atagactctc tcagcgaagg acagagacaa tacatggcaa gctggcagac
     6721 tgggacggca taagcctttt tgcgatggca aaccgtccct tctgtggccc gccgatagtc
     6781 gcgaagcaga gcaaagtggg gcgtaatgaa atcaagcagg aggtgcgaat gctcaatggg
     6841 cgcgctgttg attgaagacg taacagtgat taccatggat aaatacaggc gcataattcc
     6901 caaaggcgct gtgcttgcag agtcggggaa gataacattt gtgggttcgg tcgacgaagt
     6961 ccccaatgat cttcctgcga accttgggcg aatctctgga cgtggtcgaa ttgccatgcc
     7021 tggattcgta aacacacata cccatgctgc aatgacatta tttagaggat atgctgatga
     7081 tctgccattg caggaatggc ttgaaacgaa gatctttcct atagaggcta ggctctcggc
     7141 cgaggatgta tattggggaa ctctcct
//
LOCUS       DAOSAI010000079         7137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6733_length_7137_cov_3.265462, whole genome shotgun sequence.
ACCESSION   DAOSAI010000079 DAOSAI010000000
VERSION     DAOSAI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6733_length_7137_cov_3.265462"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLM83_04090"
     CDS             complement(<1..704)
                     /locus_tag="PLM83_04090"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOK70621.1"
                     /translation="MKLRTVKSLSGQAFRSLKKTPSVTSIIVVMACMLVLSSFMLVAV
                     NLTRIGNEFQAGVQIRAFLSPDMPPDAVDRTHAAIQAIPGVASVKYVGKDEALQRLGK
                     QLGENNFFLGDLPRNPLPESFDISLLRAADADRIAAAVLELDEVSDVRYAPKLVDRLV
                     GVLRIFWVVTGIIAVFVLVGASLIVSNTIKLTVFARRKEIEIMKLVGATDAFILFPFV
                     LEGIIIGLLGAGAAAV"
     gene            complement(694..1380)
                     /gene="ftsE"
                     /locus_tag="PLM83_04095"
     CDS             complement(694..1380)
                     /gene="ftsE"
                     /locus_tag="PLM83_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOK70622.1"
                     /translation="MVNMYGVTKVFPGNVEALRGVNLNIDKGDFVFIVGPSGSGKSTL
                     LSLIYRGTVPTRGHVLVAGRNVARLRWRDVPMLRRRIGVIFQDYKLLPDRSVYDNVAF
                     ALEVIEASPKEIEKKVPQALSMVGLAHKASAMPEELAGGEQQRVAIARAIVNDPLILV
                     ADEPTGNLDPDTTLDIMDLLCDINVRGTTVVMATHNHVVVDRMRKRVVQLERGMIVRD
                     ERWGAYCHEA"
     gene            complement(1521..2465)
                     /locus_tag="PLM83_04100"
     CDS             complement(1521..2465)
                     /locus_tag="PLM83_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOK70623.1"
                     /translation="MTKMIATRVAYGEALAELGGINSDIVVLDADLSGSTKTAGFASK
                     YPERFIQMGIAEADMMGTAAGLAATGKTAFASTFAVFATGRAFDQVRQSIAAPGLNVK
                     ICATHAGLTVGEDGGSHQSIEDIALMRVLPNMTVIVPSDAVQTGWAVRIAAEQKGPFY
                     LRLGRASVPTLYDEDADFELGKAKTLREGQDVTIIACGVMVSRALEAADRLEERGIRA
                     RVLDMHTIKPLDEEAVRAAAVETGAIVTVEEHSVIGGLGGAVCETVCSRAPAPVIRVG
                     VADMFGKSGKPDELLEAYGLTVENISFAAKRAIEMKRA"
     gene            complement(2462..3316)
                     /locus_tag="PLM83_04105"
     CDS             complement(2462..3316)
                     /locus_tag="PLM83_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOK70624.1"
                     /translation="MKRVVLSDPEELAAKACEIRKHVIRMVGAASSGHPGGSLSAADI
                     VTVLYFNKMRIRPDDPTWSGRDRFVLSKGHAAPVLYAALAEAGYFPVDDLITLRRIDS
                     YLQGHPDMNQTPGVDMSTGSLGQGLSAACGMAIAAKMDGADWRVYAVLGDGEIEEGQV
                     WEAAMTAVHRKLDNLTIFVDWNGLQIDGPIREVKSVDHIGERFAAFGWHVIEIDGHDI
                     PAIMAAIDEAQRTSGRPTAIVARTVKGKGVSFMENEAGWHGTAPKPEQVEAALAELEA
                     SRKGGVAQ"
     gene            complement(3322..5181)
                     /gene="csaB"
                     /locus_tag="PLM83_04110"
     CDS             complement(3322..5181)
                     /gene="csaB"
                     /locus_tag="PLM83_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOK70625.1"
                     /translation="MRVLVSGYHGMGNLGDEAVLAAFVQHLKEIDPKAECVALSGDPS
                     ETSAAHGIEAVPRASLGAIIREIRRSDIVISGGGSLLQDATGKKSVPYYLGIIALARM
                     MGRPVSVYAQGVGPLNRAFSRWLVKRALPRASVITVRDEASARLLQEVGVVKPEVMLV
                     ADPAFGLVPCDPPDAMAVQAPNTVMFCLRPWPGLDGREAEYALAIDRAVSELGICPIF
                     LAFQPDRDLEVAHRIADQCRAGEKSPAVISCDMVPEEVLGLISRAEMVVGMRLHSLIF
                     AANAGVPAVAIDYDPKIRAHCQRVSEPYIVEVGASADQLYAAIAGAWASRDETRARLR
                     EIMPTIGEQAKAAASISLSPILDKPVTERMKAQAEEGKDYMRASVLGSPVDCIGMDDA
                     VSILMERAALGLGAHVVTINPEMALAAKRDAELADVIRGADLVVPDGVGVVRGLRILG
                     YKPPGRVPGIELATNLMRSAAGGEMSFFLVGAAPGIAEAAAENMSKEFPGLKIAGTWH
                     GYFSDAEEEEVLRCIRDAAPNFVFVGMGAGKQEKWITKARQMAPCAVWIGVGGSFDVM
                     SGSVKRAPAAFQKLGLEWLYRLVSEPRRAKRMTALPAFVFAVMGEAFRKSRRS"
     gene            complement(5224..>7137)
                     /locus_tag="PLM83_04115"
     CDS             complement(5224..>7137)
                     /locus_tag="PLM83_04115"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOK70626.1"
                     /translation="SEIALYSRRVGINIEDCLRGLAEEGIVSVALEEDTLGSMETAGE
                     IVIIRGSEALALGSNGSPYRDILLAVAEMEVFSPSDTIVIPCNVDAATFLANRLTLRT
                     CDGPSISVIRAGDATGYAVSLPVDEARKLNLGIRPSKTAQVQAAGLRVVVRFSNFPNV
                     SEKWIDSVMTLSAVGAIPDCVIFAGTEVLGYPRFVSATVCGLDRLGTVYGDIEFQNQA
                     GGRQLARALDYKTVRVHSISRAEVDRDLSVAAMRDRYARAVRERNIRVLYMRPMMKPI
                     DERSLPEVNQSLVAEVVSAITDQGFSLGPAAPFDQLDPGLLLTLLVVLGVAAGCVVLL
                     DAIWDLPFAICIGLVAVFAAGFAALWGLGYPLLARQSVALAAAIAFPSLAAFVLFCPD
                     IPGKTRDCDTCDDGALLASKDWLGSSGTKFLTASMLCLAGGLMLAACLTSRAFMVKGE
                     QFLGVKAMHIFPMLFVLVAYWKRYARRGNESLIASLARLNRSNILVWHVIVIAILGVA
                     GLIYIARTGNTSSVPVAVPQWEQKMRAALENIFPARPRTKEFLIGHPAFVLAGALVAL
                     GETGLLLPISILGLIGQISLTNTFAHIHTPISLTLARVIIGLGLGFAIGLVATVVYRA
                     VVAQIRRAAGREG"
BASE COUNT         1665 a         2260 c         1729 g         1483 t
ORIGIN      
        1 atcaccgcag cggcgccggc gcccaaaagg cctatgatta tcccctcaag cacgaagggg
       61 aaaagtataa acgcgtcagt tgctccaacc aacttcatga tctctatctc cttgcgccgc
      121 gcaaataccg tcagctttat cgtgttggag acaatcagac tggccccgac caaaacgaag
      181 acagctatta tccctgtcac aacccagaat attcgaagaa cgcccacaag tcgatctacg
      241 agttttgggg catacctcac atcagaaact tcatccagtt cgagcacggc agcggcaatc
      301 ctatccgcgt ccgcagcgcg caacagagat atgtcgaagg attcgggtag cgggttgcgg
      361 ggaagatcgc caagaaagaa gttgttctcg cctagctgtt tccccagcct ctgcagagcc
      421 tcgtcctttc ccacgtattt caccgacgct actcccggaa tagcctgtat ggcggcgtgc
      481 gtccggtcaa cagcatcggg cggcatatcc ggcgacaaaa aagcccttat ctgtacgcca
      541 gcctgaaact cgtttcctat gcgcgtcaga ttgaccgcaa ccagcatgaa agagctgagg
      601 accagcatgc atgccattac cacgataatc gatgtcacag atggtgtctt cttcaggctt
      661 cggaaagcct ggccgctgag ggacttcacc gtcctaagct tcatggcagt aagctcccca
      721 cctctcgtcg cgcacgatca taccacgttc caactgaact actcgctttc tcatcctgtc
      781 caccactaca tggttgtgcg tggccatcac tacagtagtg cctcgcacat tgatgtcaca
      841 taaaagatcc attatgtcca atgtagtatc cgggtcaagg ttccccgtgg gttcatcggc
      901 tacgagtatg agggggtcgt tgacaatcgc cctcgctatg gccacacgtt gctgttcgcc
      961 gccggcaagc tcttcgggca tggccgaagc cttgtgggca aggcccacca tggaaagggc
     1021 ctgcggcacc ttcttctcta tctccttcgg agacgcctct atgacttcga gggcaaaagc
     1081 cacgttatca tagacggagc gatcaggcag caacttgtag tcctgaaaga taaccccgat
     1141 ccgacgccgg agcatgggaa cgtcgcgcca tctgagccgg gcgacgttgc gcccggcaac
     1201 caggacgtgc ccgcgcgtag gcaccgtccc ccggtatatc aacgaaagga gcgtggactt
     1261 tcccgagcca ctagggccca caatgaacac gaaatcccct ttgtctatat taaggtttac
     1321 cccgcgcagc gcttccacgt tgcccggaaa caccttggta accccgtaca tattgaccaa
     1381 ggtcaaccac cccatcttca gctggctata acagcatacc gtacatcaaa aacaggttct
     1441 ccttaagagg cgcaaattcc tgctgaagcg aagcggaaaa catgacgctt ccggccaaca
     1501 tctgctttta taatgtattt ctacgccctt ttcatctcta tggcgcgctt tgcagcaaat
     1561 gatatgttct caacagtaag cccataagct tccagaagtt cgtcgggctt gccggacttt
     1621 ccgaacatat ccgctacgcc cacgcgtatc actggcgccg gagcacgtga acacacggtt
     1681 tcgcataccg cgccaccaag gcctcctatg actgagtgct cttccactgt gacgattgcg
     1741 ccggtttcga ccgccgccgc acgcacggcc tcttcgtcca agggctttat ggtatgcatg
     1801 tcaagcacgc gcgcccgaat ccctctttcc tcgagcctgt cggcggcttc gagggcccgc
     1861 gataccatga ccccgcacgc aattatcgtt acatcttgcc cttccctcag cgtcttcgcc
     1921 ttgccaagct cgaaatcggc gtcttcatcg taaagcgtcg gcacggaagc ccggcccagt
     1981 ctgaggtaga acggtccctt ctgttccgcg gcaatacgaa ctgcccaccc agtctggact
     2041 gcatcagaag gcactatcac agtcatgttg ggaaggactc tcatcaacgc gatgtcctcg
     2101 atagactggt gagaccctcc atcctctccc actgtaagcc cggcatgggt cgcgcatatc
     2161 ttcacgttca gtccaggcgc ggcgatgctc tggcgcacct gatcgaaagc gcgcccagtg
     2221 gcaaatactg caaacgtgct tgcaaacgct gtcttgcccg tggcagcgag acctgcagca
     2281 gtgcccatca tgtcggcctc agcaattccc atctgtatga agcgctccgg gtactttgag
     2341 gcaaacccgg cagtcttggt cgatccagaa aggtcggcat cgagcactac gatgtccgag
     2401 ttgatcccgc ccaactcggc aagggcctct ccataggcca ctctggtagc tatcatcttt
     2461 gtcattgcgc cacaccgcct tttcgtgatg cttcaagttc cgccaatgcc gcctctacct
     2521 gttcgggttt gggagcagta ccgtgccatc ctgcttcgtt ctccataaag gagactcctt
     2581 tgcccttaac ggttcgagcg actatcgccg taggcctccc gctggttcgc tgggcctcgt
     2641 ctatagccgc cattatcgcg ggtatgtcgt ggccgtctat ctctattaca tgccatccaa
     2701 acgcggcaaa ccgttcacct atatggtcaa cagacttcac ttcgcggata ggcccatcaa
     2761 tctgaagtcc attccagtcc acgaatatag tcaggttgtc cagcttcctg tggacagcgg
     2821 tcatagcggc ctcccacacc tgaccctctt cgatttcgcc atcacctaag acggcataga
     2881 cgcgccaatc agcgccatcc atcttcgcgg caatcgccat gccgcacgct gcagagagcc
     2941 cctgtccgag ggatcctgtg gacatgtcca cgcccggcgt ctgattcatg tcgggatgcc
     3001 cctggagata actgtcgatc cttctcaaag taatcagatc atccaccgga aagtaacctg
     3061 cttcagccag tgcggcataa agcacgggag cggcatgtcc cttcgacaac acgaatctat
     3121 cgcggccaga ccatgtcgga tcatccggac gaatcctcat cttgttgaag taaagcacag
     3181 tcacaatgtc agcagccgac aatgagcctc cagggtgacc agatgatgcg gcgccaacca
     3241 tgcgaattac atgcttgcgg atctcgcacg ccttagcggc gagttcctct gggtctgaga
     3301 gcacgactct tttcaacgtc atcaactcct tctgctcttg cgaaaagcct cacccatgac
     3361 tgcaaagacg aatgcgggta aagccgtcat cctctttgct ctgcgaggtt ctgatactag
     3421 cctatataac cactccaagc ccaacttctg aaaggcagcg ggcgcccgct tcacacttcc
     3481 ggacatcaca tcgaaggagc ctccgactcc tatccatacc gcgcatggcg ccatctggcg
     3541 cgccttcgtt atccattttt cctgctttcc cgctcccatt cccacaaaga cgaagttagg
     3601 ggcggcatcg cgaatacagc ggagaacttc ctcctcctcg gcatctgaga aataaccgtg
     3661 ccaagttcct gcaatcttga ggccagggaa ttccttgctc atgttctctg cagctgcctc
     3721 ggcgatgcca ggtgccgcgc ctacaaggaa gaatgacatt tcgcctccag ccgccgagcg
     3781 catcaaattc gtcgccaact ctatcccagg caccctcccc gggggcttgt atccgaggat
     3841 tctcagcccg cgcaccacac ccacgccatc tggaacaacc aagtcagccc cgcggataac
     3901 atcagcgagt tcagcatcgc gcttggctgc aagcgccatc tcggggttta tcgtcaccac
     3961 atgggctccc aagcctaaag cagcacgctc catcaatatg gatactgcat catccatgcc
     4021 aatacagtcc accggcgacc caagaactga cgccctcata tagtctttgc cttcctccgc
     4081 ttgggctttc attctttctg tcacgggctt gtccaatata ggggaaagcg agatgctcgc
     4141 agccgccttc gcctgctctc ctatggtcgg cataatctcc ctcaacctcg ccctcgtttc
     4201 atccctagat gcccacgcac cggcaatcgc cgcgtagagt tgatcagctg acgcccccac
     4261 ttccactatg tacggttccg acacgcgttg gcagtgcgcc cgaatcttag gatcgtagtc
     4321 tattgcgaca gcgggcaccc ctgcatttgc ggcaaatatc agagagtgca gcctcattcc
     4381 caccaccatc tcagctcggg atatcaaccc aagcacttcc tccggcacca tgtcacacga
     4441 gataacagca ggactttttt cgccggccct acattgatct gcaattcggt gagccacctc
     4501 aagatcgcgg tcaggctgga acgccaggaa aatagggcat atgcccagct ccgatactgc
     4561 acggtcgatg gctaacgcat attcagcctc gcgaccatca agccccggcc atggcctcaa
     4621 acagaacatc accgtattcg gtgcttgaac agccatggcg tccggcgggt cgcagggaac
     4681 aagcccaaaa gcgggatcgg caactagcat gacctcaggc ttcacaacgc cgacctcctg
     4741 cagcagacgg gcagaggcct catcccgcac agttatgacg gaggcccgag gaagcgcgcg
     4801 cttcaccagc catctcgaaa aagcacgatt caaagggcca actccctgtg cgtatactga
     4861 aaccggccga cccatcatcc gcgcaagggc aatgatgcca agatagtatg ggaccgattt
     4921 cttgcctgtt gcgtcctgca agaggctgcc cccgccgctt atgacgatgt cagaccggcg
     4981 aatctcgcga atgatagctc caagcgacgc cctaggcacc gcctctatgc cgtgtgccgc
     5041 gctagtctct gatggatcgc cagagagcgc cacacactct gccttcggat ctatttcctt
     5101 gaggtgttgg acaaatgcgg caagcaccgc ctcgtctccg agattcccca ttccatggta
     5161 gccggagacc aagacccgca tactgcactc acctgctttc ccacgcacga cacttactca
     5221 agatcatcct tcccggcccg cagcccgacg tatctgggca actacggccc tatagaccac
     5281 ggttgcaaca agaccgatcg caaacccgag ccccagtccg ataatgaccc tggccaatgt
     5341 gaggcttatg ggagtatgga tatgagcgaa cgtgttcgtc agcgaaatct ggcctatgag
     5401 gcccaaaatg gagatgggca acagtagacc agtctccccc aaagcaacaa gagcgccagc
     5461 aagaacaaaa gccgggtgcc cgatgaggaa ttccttagtt cggggccttg cggggaagat
     5521 gttctccaaa gcagccctca ttttctgctc ccattgggga accgctaccg gaacagacga
     5581 tgtattgcca gttctcgcaa tgtatatcag ccctgcaaca cccagaatcg caatgacaat
     5641 cacgtgccac acgaggatat tggatctgtt gagcctagcc aatgaggcta tgagcgattc
     5701 attgccccgt ctcgcatacc gcttccaata agccactaac acgaacaaca ttgggaagat
     5761 gtgcattgcc tttaccccca aaaactgttc gcctttgacc atgaacgcgc ggctcgtaag
     5821 gcatgcggca agcatgagcc caccggcaag gcatagcatc gatgcggtaa ggaatttggt
     5881 tcctgaagat cccagccagt ctttgcttgc caagagcgcc ccatcgtcgc aagtatcgca
     5941 atccctggtt ttcccgggaa tatcggggca gaacagaaca aaagcagcga gcgagggaaa
     6001 cgcaatggct gcagcgagcg caacagattg cctcgccaag agcggatatc ccaatcccca
     6061 aagagcagca aatccagcgg caaacaccgc aacaagccct atgcatatgg caaacggcag
     6121 atcccaaatg gcatcaagca acactacaca tccagccgca acacccaaga ctaccagaag
     6181 cgtcagaaga agcccaggat caagttgatc gaatggtgca gccgggccaa gcgaaaagcc
     6241 ttggtccgtg attgcgctaa ccacttctgc caccaaagac tggttaacct cggggagact
     6301 gcgttcgtca atgggcttca tcataggacg catgtagagc actcgtatgt tgcgctcccg
     6361 gacagccctg gcatacctgt ctctcatcgc agctacagaa agatcgcgat cgacttccgc
     6421 cctgctaatg ctgtggactc gcactgtctt gtaatcgaga gcccgggcca gctggcgtcc
     6481 gcctgcttga ttctggaatt ctatgtcgcc ataaactgtt cccaagcgat ccagtccgca
     6541 cactgtagcg gatacaaatc gcggataacc caggacttca gttccagcga atatgacgca
     6601 atccggaata gcaccgacag ctgacaatgt catcacagag tctatccact tttccgacac
     6661 attggggaag ttggaaaatc taacgaccac acggagcccg gcagcctgca cttgcgccgt
     6721 cttcgacggg cggattccca gattgagctt gcgtgcctca tcgacaggaa gagagactgc
     6781 gtatcccgtt gcatctcccg ccctgatcac ggagatggaa ggcccatcgc acgtacgcaa
     6841 agtcaagcgg ttcgcaagaa aagttgcggc gtccacattg cacgggatga caatagtatc
     6901 cgaaggtgaa aagacctcca tctctgcaac cgccaacagg atatccctgt acgggcttcc
     6961 gttcgatccc agtgcaagcg cctcggagcc cctgatgatc acgatctccc cagccgtctc
     7021 catcgagcct agggtatcct cctccaaggc gaccgagaca ataccctcct cagcgaggcc
     7081 tcgcaggcag tcctcgatat tgatgccaac gcggcgactg taaagagcga tttcgct
//
LOCUS       DAOSAI010000080         7079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6810_length_7079_cov_4.534880, whole genome shotgun sequence.
ACCESSION   DAOSAI010000080 DAOSAI010000000
VERSION     DAOSAI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6810_length_7079_cov_4.534880"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1558..2508
                     /locus_tag="PLM83_04120"
     CDS             1558..2508
                     /locus_tag="PLM83_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOK70627.1"
                     /translation="MKVGDIEKIGVAGAGTMGSGIAQVFASKGYEVVVTDIAEEQLRR
                     SAKLVDIFNSSLIEEGIMLPEEAQKVKGLISYSTDKRDFSDADLIIEAIVEKMNIKQD
                     FWHETEAIAKEDAFFATNTSGLSINGICEKVENKERFIGMHFWNPSHIIPLVELTKAD
                     ETSDETVEELKKLMATIEKEAVVVQKDAPGFIGNRLQFAVFREALYIVDEGIATFEDV
                     DKAMKYGPGFRYPVIGPLQTADLGGLDTFYYISDYLFEELSDMKKPPAMLQSMMDAGR
                     LGVKSNKGFWDYSGDKQAEAIKTRDKMLFDMLKWSKKVRK"
     gene            2719..2922
                     /locus_tag="PLM83_04125"
                     /pseudo
     CDS             2719..2922
                     /locus_tag="PLM83_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589169.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     gene            complement(3063..3521)
                     /gene="bcp"
                     /locus_tag="PLM83_04130"
     CDS             complement(3063..3521)
                     /gene="bcp"
                     /locus_tag="PLM83_04130"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOK70628.1"
                     /translation="MAKLEIGQPAPDFALPNQDGKMVRLSDFRGRKLVAYFYPRDNTP
                     GCTREAEGFRDLHDQIVAAGAEVVGVSKDSVSSHAKFHSKLQLPFDLLSDPNHSVLEA
                     YGAWGTRTMYGKTSEGTIRSTVIVDEEGRVAAVFPKVKPDGHAQEVLETI"
     gene            complement(3935..4363)
                     /locus_tag="PLM83_04135"
     CDS             complement(3935..4363)
                     /locus_tag="PLM83_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70629.1"
                     /translation="MDRKDVLTRILAILGTVLVWTPILFTVLTSIVGTISSRVFRFDY
                     LMPAELFPIAFVGALLLLWAARRAKSHQKLIGWGLGGSVAFLAGGQLYAVITGLASGV
                     TEPAGFVWAVALASIALYSLALVMTGIAGMSLVRWLYLSQ"
     gene            5408..6706
                     /locus_tag="PLM83_04140"
     CDS             5408..6706
                     /locus_tag="PLM83_04140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOK70630.1"
                     /translation="MNRSAHRFPFLRAMDDPGHIQNVKNPKYVELLITTKLEGIDVVP
                     QAVYFLSFAAIGVPLCINTMLTRPLPEPATSVLLLVLGLISYHVPVTFPSNVQFHPGF
                     PLLMGALYCHGCGAANLVIMPSTLLHVFTRNHGLFNCFFNVGQFALCICAAETVGFHL
                     GWEPGAPATATDLLPISLMMLTFDLLNVLFVSVSRSIENKEPWKKCFTKLLYTDRKAV
                     LPQRTFLTVVSMLLSSHMGDIAFVIVFIGVISLRFQNAFQRELLEKTEEAETDLLTGV
                     YNIRYLQKWLNTKCKAATGAEGVCSFIFADVDGLKTINDEYGHEIGNKLLIHVARLLL
                     ANVRSKDRVVRYGGDEFVIAFPGIGLPQSTAAAMRILQASDSAPFSVNGAEVDVGISI
                     GVASWPEHGETVFDVIRIADRAMYVAKRSGGNRVQSAADL"
BASE COUNT         1774 a         1665 c         1891 g         1749 t
ORIGIN      
        1 ccttcggagg tctgttttgg aaattctctg cctccttatt aactctttcg tattaatggc
       61 taacgagtcc tcttttttcc tttgtagaga ctctaatcat atgatgaggc gccctgaaaa
      121 cccttgcagc aacaatgaaa tcctatgtgc tccaatgggc attgcaacga gcgctattgc
      181 catccagcgc caacagggcg atgtcgctac aagatggatt atgcgtcgaa aacccagtca
      241 taccatgtga ttccgtatat gtctgaacac cgcaaattta cagtttgccg tttctcgaaa
      301 ttgtgcttgc ctgaagctgc ttctgaagcg ctttttcttc cttgcccagc agtctgtcgg
      361 ggcttttggc aaatgaacgc agtcttttag caagggttga tgctcctgcc aatgtggtct
      421 tactgcattg ttgatcgcac caatatcttg gagccggaaa cctggcgaca gatgaaagca
      481 agtctgcggg gatgaggaaa gaagaacctg tgcttacaca cagcgacagg tagccttcag
      541 tcgtatcgat ccccaaccac aggatctaga tctaaataca ctggggattt caggtttcga
      601 acgcaagccc tgcgagatcg tcattatgca gaagcgttaa ctggctcttc agaggcgata
      661 aaaggggcaa tatgaaggac ttgactcaat cattgcaggc agggatactg ccaaatttgt
      721 ggaagaactc gtatgatgag ggattctgtc agaggaacta cgttccataa atgtaattgg
      781 ataacgtctc ctcgaatcca tgagacagat cgcgattgtc acgccgatta tggcaattgc
      841 tgattcatag ggtgcaaccg ttgcctggag gttcaaacag ctcaaaatgg gagatatttg
      901 tggtcggcgc ggtgggatct gcgattctta tcagtagcgg gaaaaccctt attcgacttg
      961 ttggcgccag cataatggcg cctttttcat gggtagccgt tgtttgcctc ggcattcatg
     1021 ccatacagct cgttttggca tagtcaattt ctctaagtaa gtagctcata tcccgggcta
     1081 tccgttgagt tcactgctat tgcccgtctc tagttggctt gttgcggttc ggatctgatt
     1141 gggcggctcg atggtccgaa ggatggaaac gagcgggcga ggagctgtga gtgcttacta
     1201 tacgcggtat cgatagttac aggcaagcaa cctcaggctg aaactgaaag catcggtgaa
     1261 tcccacttca gtatggcctg tggattttcc gggacagcct cagagcgcgc gatagagacc
     1321 attacgactt gttcattaag attgcaaatg acgataactg ttcttgattg ggttgccgat
     1381 tttctatttc atgcaaggat tcagcttttg ccggacagta aatcgcacct atggagaata
     1441 tgatgatcag cttatggggg tatgcgggag aaagcttctt gactaatgct atcgtcagaa
     1501 tcggctgcta taggcttgcc ttcaacgaga gatgtgacga cgaggagagt gattgtaatg
     1561 aaggtcggag acatagagaa aattggcgtt gccggagccg gcaccatggg ctcgggaata
     1621 gcgcaagttt tcgctagtaa aggctacgag gtagtcgtaa cggatatagc cgaagaacag
     1681 ctgcgaaggt cggccaaact tgtagacatt ttcaacagca gtcttatcga agaagggata
     1741 atgcttcccg aagaggcgca aaaagttaag ggactcatat cttatagtac agataaacgg
     1801 gatttcagcg acgcggacct cattatcgaa gccatcgttg aaaagatgaa cataaagcaa
     1861 gacttttggc atgaaacaga agcgattgcg aaagaagatg ctttctttgc tacgaatacg
     1921 tcagggctca gtataaacgg tatatgcgaa aaggttgaga acaaggaaag attcattggc
     1981 atgcactttt ggaacccgtc ccacataatc ccattggtcg aactcaccaa ggcagatgaa
     2041 acatctgacg agactgtcga ggaactgaaa aaactcatgg cgaccataga aaaggaagct
     2101 gtcgtcgtgc aaaaagacgc ccccggtttc atagggaata ggctccagtt tgcagtcttc
     2161 agagaggctt tatacatagt ggatgaaggg atcgccacct tcgaagacgt tgataaggcc
     2221 atgaaatatg ggccgggctt caggtatccg gtaatagggc cattgcagac tgcggattta
     2281 ggaggactag atactttcta ttacatatct gattaccttt ttgaagaatt gagcgacatg
     2341 aagaaaccgc cggcgatgct tcagtcaatg atggatgcgg gacgattggg ggtcaagtca
     2401 aacaaggggt tttgggacta ctcaggtgac aaacaggcag aggccataaa gacaagggat
     2461 aaaatgctct ttgacatgtt gaagtggagc aaaaaggtgc ggaaataggt tagcagtcta
     2521 caactttgcc cacctagggc gtatttgttc aagaagattt gcgggtgggc attattatct
     2581 ctctggtgct gctcatgttt ccagcctaga caggtcattc ctgttcccat tgtcgttgct
     2641 acaggcgttg aggacgccta catgactgga tacgccgcgg tgccatcttc ggccaattaa
     2701 tggactatgc tggagaggct caccagaata tctgggcttg aaatagccag ctgaggagtg
     2761 atcatcagca gcgctgtgct tacatatgac gaaatcagcc tgtttgtggt cgtatttgcc
     2821 atgtatacgc tggcacttgc gctgtttggg gaggctaaca tctcaaggac tctaatctta
     2881 tgttcaattg gactgggctc attcacgttt accatgaccg ccgcggtaat aggcctggtg
     2941 gccgatggtt tagggttagc accgtagtct acgttggctt tgaaacagag ttctctgtca
     3001 gacgacggcg tggacagctg atttgacact gtttcggaca ggggcaggca acgtgtggct
     3061 gctcagatag tctccagcac ttcttgggcg tggccgtcag gtttcacctt gggaaagacg
     3121 gcggctacgc ggccttcctc gtctactatc acagtggacc gtattgtacc ctcagatgtc
     3181 ttcccataca tcgtcctcgt gccccaggcc ccgtatgctt cgagaacgct gtgatttgga
     3241 tcgcttagaa gatcgaaggg cagctgtagc ttgctgtgga atttggcatg ggatgacacc
     3301 gagtctttgc tgaccccgac aacttcagcg ccggccgcga cgatctggtc gtgaaggtcg
     3361 cggaagccct cggcttcacg ggtgcacccg ggtgtattgt ctcttggata gaagtatgcg
     3421 acgagtttac ggccccgaaa gtccgagagg cggaccatct ttccgtcttg gtttgggagc
     3481 gcgaagtctg gagcgggttg tcctatttcc agtttggcca tgcatgaact gcctcccttc
     3541 atgttctgac tgtagtgtag cagagtccgg acatgcaggg caatcgcctg atttactttg
     3601 catttgccgc agtccatgct tgacgaagcc gagggagttt ttgtgagccg tgtaggtttt
     3661 ctgacgcgct cagcaatctg acccgaagca tttggctatc ccccggtcgt caccctgaac
     3721 aattctcata tggcatctgc gtgataggat aatctgttgc gcctgtggac aatacttgaa
     3781 cgttgcccaa ggccgctcag ttgaccgggt ggaggtggtt tggcttggga tggattctgg
     3841 caagaaacag atatagcagg agtgcggtga aaaaaagcag cctagaaaga aattgggctc
     3901 aagcggtagt tgcagataac cctgtcttcg gatgctactg ggaaagatac agccatctca
     3961 ccagagacat gccggctata cctgtcataa caagcgctag cgaatagagg gcgattgaag
     4021 cgagggcaac tgcccaaacg aatcccgccg gctcagttac gcccgaggcg agccctgtaa
     4081 tgacggcata gagttggccg ccggcaagaa acgccacaga gccgcctaaa ccccagccga
     4141 tcagcttctg atgagacttc gcccgcctcg cagcccacaa cagcagcagg gcgccgacga
     4201 aggcaatggg aaaaagctcg gctggcatca gatagtcgaa ccggaatacg cgggaagata
     4261 tcgttccaac tatggacgtg aggaccgtga acaagatggg ggtccacacg agcaccgtcc
     4321 ctaagatggc cagtattctg gtgagcacat ccttgcgatc catttgtggt ccctccctct
     4381 agggcttcaa ggttccattg caggcgcctt tctaccttct attatacata tgccgcaagc
     4441 caaagacgaa gaagggccga aaagaggcta aggagcgccc tcatcaatga ggaagcattt
     4501 gggacgttgg ccagtccaca gcgggattga gattgagaaa tgcgccggcg agttgatttc
     4561 ggtgcgcagt tgtgcctgag aacggttgca gacatgtcag atcatgaaga cgggatgaga
     4621 tggtgctgca gacactgtgt atcaaggacg tagccgcggg ggcacattca tcttgagaat
     4681 catgcaaggc aacaagactt gtttgttcag gtctcgctct aagggatgat tctgcgcgtg
     4741 gcgcgtgctt cttctcacag tgtgtctgtc caatctgtgc ggtgcctttg cggaacgagg
     4801 tccatatcga ggcgcctgtc gtctgcagaa tgttatcccc aactacgtat acgagccgat
     4861 gcaaagacga gtatggaaaa cgccgtaagc tgcctgcaac gccagagcaa gcgcacaata
     4921 cttaatgccg tgcctgtgca gcttctgacg aatgtcctgc gccatctctc aaaactgcga
     4981 aaatggcaat gcaaaccgtg cgctcatgcc aattggtccc atgtcagcac ttgaaatatt
     5041 ccaatgatag taatacgata accgtaacga aagtcactat cattggagaa aaacactatt
     5101 cttgccaaca cacaacaatc tgctgcaaaa agtgagcgtt tggcatgtcg agtggcctga
     5161 ggcaagcacg caataccgga ttgaacatct cccttagcgg ggactggttt gtgttcggac
     5221 gtgctagttg tcggtcgaaa ctgctttaat cacagccgtg atgatcacta cagtgcttac
     5281 caaaaccctg ctgtaaaatc tattagtcac aggtgagccc ctcaagcggg attcatcgat
     5341 gcctcaagtc tgagtcagag tttgcttacc aatagcatgt tcgcataaaa aaggagggac
     5401 aagaggtttg aatcgttcgg cccatcggtt cccgttcctg cgggccatgg atgatccagg
     5461 ccatattcag aacgtgaaaa atcccaagta tgttgagctt ctcatcacaa ccaaacttga
     5521 gggcatcgac gtagtgcctc aggctgtcta ctttctgtcc tttgcggcta ttggtgtgcc
     5581 tttgtgtatc aatacaatgc tgaccaggcc cttgcccgaa ccagcaacct ccgtgttgct
     5641 gttggttctt gggcttatct cgtaccacgt cccagtcaca tttccgtcaa acgtccagtt
     5701 tcacccgggc tttccccttc tcatgggcgc gctttattgc cacggttgtg gcgcggcaaa
     5761 cctcgtgata atgccctcga cgctgcttca cgttttcacc agaaatcatg gactctttaa
     5821 ctgctttttc aatgtaggcc aattcgcttt gtgcatctgc gctgcggaaa ccgtgggttt
     5881 ccacttgggc tgggaaccag gtgctcccgc gacagccacg gatctcttac caatatcttt
     5941 gatgatgctg acatttgacc ttcttaacgt cctgttcgtc agcgtatcaa ggagcataga
     6001 aaacaaggaa ccttggaaga aatgctttac caagttgctg tacacagaca ggaaagctgt
     6061 attgccccaa cgtactttct tgactgttgt ttccatgctg ctctcatccc acatgggaga
     6121 cattgcattt gtcatcgtgt tcataggtgt aatatctctc aggtttcaga atgcattcca
     6181 aagagaatta ttggaaaaga ccgaggaggc agagaccgat ctgcttaccg gagtctataa
     6241 cattcgctac ttgcaaaaat ggctcaacac caagtgtaag gcggcaaccg gcgctgaggg
     6301 cgtctgttcc ttcatattcg cagacgttga tggactgaag actataaatg atgagtatgg
     6361 acatgaaata gggaacaaac ttctcatcca tgtggcgagg ctccttcttg ccaacgtccg
     6421 aagcaaggat cgggttgtaa gatatggggg agatgaattc gtcatagcct ttcccggtat
     6481 aggcctcccg cagtctactg ctgctgccat gaggatcctg caagcttcag acagcgcacc
     6541 attttcggtt aatggcgcag aggtggatgt tggcataagc attggagtgg catcatggcc
     6601 ggaacacggc gaaacagtat tcgatgttat ccgcattgct gacagagcga tgtacgtggc
     6661 caaaagaagc ggagggaaca gagtgcaatc agctgctgat ctgtgatgag actgaccagt
     6721 ttcatggttt gcctggggcg gataggcagt cacagcatcc agccccaggc aaaccagaca
     6781 atacatagga agcgtcagca ctcaagaaga gcgaatgggt tccaacgagc ctaacccgag
     6841 atcgtgtgaa taggattgcg ctggtggttc gaagaggaga gccagggatg cggtcgcctc
     6901 aaacgccaga tcgggcaagc tcttcggcga tctgaagggc cgcccgatgc tggctgtggt
     6961 ttgcaaatta taggtagaca acctgaaatc agaatgcctt caaatatgac ctggcatcca
     7021 tgcgtcttct cacctagtgt atcctctttg gctgcaaggg taagggttgc aatcctcaa
//
LOCUS       DAOSAI010000081         7075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_6818_length_7075_cov_3.947721, whole genome shotgun sequence.
ACCESSION   DAOSAI010000081 DAOSAI010000000
VERSION     DAOSAI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6818_length_7075_cov_3.947721"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..>2501)
                     /locus_tag="PLM83_04145"
     CDS             complement(194..>2501)
                     /locus_tag="PLM83_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOK70631.1"
                     /translation="WPNPQVSIEDTPESVAISTPAVIVDISKDPLRIAFYNPDGALIC
                     SDSAPRGMGWDELGAGCYKSFHPHTHFYGFGERTGGLDKRGTTMTLWNTDNCPYTPGT
                     DPLYISIPFFIAFSGESAYGLFMDTAARSTFNMGKPHPMLNSSKIPPQPTGIPEVPEG
                     EYWFGTSQKKLDYYFFAGPSIPDVVRGYAALTGYMKLPPLWALGYHQCRFSYCPDSQV
                     LEVAKRLREADIPCDAIYFDIDYMDGFRVFTFEPEDFPDPKELISQLSDMGFKVVAIV
                     DPGVKVDPDYSVYDKGRANQYFVKKADGKDYKGTVWPGTVSYPDFARADVRSWWAENH
                     SALFDAGVRGIWNDMNEPSNFDTESKTMDEDALHGSRGEEVAHAYVHNAYGNLMSEAT
                     QEAFRRLLPKTRPFIISRAGCGGIQQSACVWTGDNSSWWEHLLMSIPMCLNLSLSGVS
                     FVGADVGGFLYDSDPELVTRWTQLGTFIPLFRNHSAIWTVPQEPYSLGEPYASICREF
                     IKIRYRLIPYMYTLMKECADDGTPVMRPMVFEFPDDEKTHTMSDQFMLGPDMMIAPIY
                     MPGADCRPVYFPRGSWANLFNGNVISSLGESVLVGASLGQIPVFLREGAIIPWGMEMS
                     YVGQHRQALEIIDIFPEEGPWERTFSLYVDDVETLGYQEGKFGSVEISCRTSERETGR
                     VLEISIDAQEDDYACAMAVRTLRIWNVAEEPVEVSVDGQRLPMLGNVCEVAGGTGQTG
                     YSYDEAGSRLCIGVHKISRNLEIVVAYR"
     assembly_gap    2502..2511
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2952..4112)
                     /locus_tag="PLM83_04150"
     CDS             complement(2952..4112)
                     /locus_tag="PLM83_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70632.1"
                     /translation="MSVLNRNALLARKFYRAKLTAQHADNMIKISFTRIRGCTIVLVR
                     DSTISMSSRYARMRQESVSESVRVRIGPETRPSAATSLVRDHVSISDSARAALARADN
                     SMSTDKSQALKGIPTDEASNDPKIRTIKRIIELLTGIKIETIELSEVVRSRQTQTPQS
                     MPEPEWSIEYDREYSYHDFQYMSFEAEGWITTEDGEQIKFSLSLSMRHEESAYSATSI
                     HIQNGRKIDPIIVNLDGEAAQLSDELFEFDLNADGKTEMIPGLHRGSGFLVLDRNGDG
                     IVNDGTELFGPSTGNGMNELAELDSDGNRWLDERDPAWTQLYIWTGGNPGDEPLVSLA
                     EAGIGAIYLGSVGAQFQMKNSANRPVGELARMGLYVRQNKTVGTMEQIDLIV"
     gene            4318..4515
                     /gene="rpmE"
                     /locus_tag="PLM83_04155"
     CDS             4318..4515
                     /gene="rpmE"
                     /locus_tag="PLM83_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOK70633.1"
                     /translation="MKDNIHPKFYITTVTCACGESFETGSTRENMRVDICSKCHPFST
                     GVQRVVDTGGRVEKFRKKHNL"
     gene            4640..>4796
                     /locus_tag="PLM83_04160"
     CDS             4640..>4796
                     /locus_tag="PLM83_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOK70634.1"
                     /translation="MVGTGWIEVICGSMFSGKTEELIRRVKRARIARQNVVAVKPALD
                     IRFSSKDV"
     assembly_gap    4797..4806
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4807..5258
                     /locus_tag="PLM83_04165"
     CDS             <4807..5258
                     /locus_tag="PLM83_04165"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOK70635.1"
                     /translation="VVSHDGDKIGCLPVKDSDEAEAIIPDDVQVVAFDEAQFFDSGIV
                     DLCVRLADRGVRVIVAGLDCDFRGEPFGSMPELLARAEYVTKLSAICVKCGNPATRTQ
                     RIIDGRPANYSDPQVVIGASEMYEARCRRCHQVPGKPTRNRTGGNQG"
     gene            5255..6142
                     /locus_tag="PLM83_04170"
     CDS             5255..6142
                     /locus_tag="PLM83_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOK70636.1"
                     /translation="MNPRYQYGGQAVIEGVMMRGRDSAAIAVRRPDGDISVKMRDIDS
                     SKRPAILKAPFIRGVVTLIESLVVGMESLMYSANESSEEEEQLSGGEVVLSLVMAFGL
                     FILLFIVLPNVIVAFMQKHIPSVVLVNLLEGVLRIAIFLVYIVAISKMEDIQRVFAYH
                     GAEHKTIAAWEAGEELTVDNARKYSTLHPRCGTSFLLIVMVLSILVYSFLGEQTLGER
                     ILSRLILLPVLAGLSYEVLKLAGRPNPSVFVKWLSAPGLALQKLTTREPDDSMLEVAI
                     ASLNAVIEKDQASSEAAVI"
     gene            6144..>7075
                     /gene="prfA"
                     /locus_tag="PLM83_04175"
     CDS             6144..>7075
                     /gene="prfA"
                     /locus_tag="PLM83_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOK70637.1"
                     /translation="MIGKLDEIEARYVELEQQIADPAVIADQITYRKLAKAHSDLEEI
                     VGKYRKLKDVMAEIVSVKEMLDGHLEPDMRELAEAELDELESRRGALESELRVLLLPS
                     DPRDEKNVIVEIRAGTGGDEAALFVGDLFRMYARYAERHGWELESMASSPTELGGFKE
                     IIFMISGDKVYSRLKYESGVHRVQRVPETEASGRIHTSAATVAVLPEAEEVDVEIDPN
                     DLRIDVFRSSGHGGQSVNTTDSAVRITHIPTGMVVTCQDEKSQHKNRDKALRVLRARL
                     LEQLTEEQEADIAETRKSQVGSGDRSERIRTYNFP"
BASE COUNT         1558 a         1762 c         2040 g         1695 t
ORIGIN      
        1 gggcgcgggt gtctaagttt tagactggtc tgcaaaacct gctcgccccc gcggcaaacc
       61 ttggccggct ccacgctgca cggcgcttcc ctgggctttt acccaaggcc aaacttacaa
      121 gttcagcccc cggctaagtc ccagacagcg acagcacaac tcgaggatcg cgcagagatc
      181 tgtcgcggtc tacttagcga tatgccacga ctatctccag attcctagag atcttgtgga
      241 cgcctatgca aagcctactt cctgcctcgt cgtaggaata ccctgtctgt ccggtgcctc
      301 ctgcaacctc gcacacattg ccgagcatag gtaggcgttg accgtctacg ctcacctcaa
      361 ctggttcttc agccacattc catatgcgca gggtccttac ggccatcgcg catgcgtagt
      421 catcttcttg ggcgtcgatg gagatttcta ggactcggcc ggtttccctt tctgatgtac
      481 ggcaggatat ctccactgaa ccgaatttgc cctcttgata gccgagggtc tctacgtcat
      541 ccacatatag tgaaaacgtg cgctcccaag gaccttcctc tgggaagata tcgatgatct
      601 ccagcgcctg tcggtgctgg ccgacgtaac tcatttccat cccccacggg attatggcgc
      661 cttcgcggag gaagaccggg atctggccga gggaggcccc cacgaggacg gattccccaa
      721 ggctcgatat cacgttgcca ttgaacaggt tggcccaact gccccttggg aagtagacgg
      781 gtctgcaatc agcgcctggc atgtagatag gcgctatcat catgtcggga ccgagcatga
      841 actggtcgct cattgtgtgc gtcttttcgt cgtcagggaa ctcgaacacc attggcctca
      901 ttacaggggt tccgtcatcg gcacactctt tcatcagtgt gtacatatat ggaatgagcc
      961 tatagcggat cttaatgaat tcgcggcaga ttgacgcata cggttctccc agagaataag
     1021 gttcctgcgg gacagtccag atggctgaat ggtttcggaa gagcggaatg aatgtgccaa
     1081 gctgagtcca acgggtgact agttcagggt ctgaatcata caggaaccct cctacgtcgg
     1141 cgccgacaaa tgacactccc gaaaggctta gattgaggca catcggaatg gacatgagga
     1201 gatgctccca ccagctcgag ttgtctccag tccagacgca cgcgctttgc tggattcctc
     1261 cgcagccggc tcgtgagata atgaatggtc gagttttagg caggagcctg cggaatgctt
     1321 cctgtgtagc ctcgctcatg aggttgccat aagcattatg gacgtaggcg tgagccacct
     1381 cctctcctcg agagccgtgg agagcatctt cgtccatcgt tttgctttct gtgtcgaagt
     1441 tagacggttc attcatgtca ttccatatgc cccgcactcc tgcatcgaaa agggcggaat
     1501 ggttctcggc ccaccaggag cgcacgtcgg cccgggcaaa atcggggtat gagactgttc
     1561 cgggccacac tgttcccttg taatcttttc cgtcggcctt tttgacgaag tattgattgg
     1621 ctcggccttt atcgtaaaca gaataatcgg gatcaacctt gactccggga tcgactatgg
     1681 cgactacttt gaagcccatg tcggaaagct gagaaatgag ctctttggga tccggaaagt
     1741 cttccggctc aaaagtgaat actcgaaaac cgtccatgta atcaatgtca aagtatatgg
     1801 catcgcaagg aatatctgct tccctcaatc gtttggcgac ctccagaact tggctgtcag
     1861 ggcagtagct gaacctgcac tggtggtagc ctaatgccca cagaggcggc aacttcatgt
     1921 agccggtcag tgctgcgtag cctcggacta cgtctggtat gctagggcct gcgaagaagt
     1981 agtagtccag cttcttttgt gacgttccga accagtattc gccctctggt acttcgggaa
     2041 tacctgtagg ctgaggcgga atcttggagc tgttcaacat gggatggggt ttgcccatgt
     2101 taaatgtgga ccttgctgct gtgtccatga acaggccgta ggcggattcg ccggagaaag
     2161 ctatgaagaa tgggatagat atgtaaagtg ggtctgtgcc cggcgtgtac gggcagttgt
     2221 cggtgttcca aagggtcatg gtcgtgcccc gcttgtcaag gccgcctgtc cgttccccaa
     2281 acccatagaa gtgagtatga ggatggaagg acttgtagca ccctgcgcca agttcatccc
     2341 agcccattcc tcggggggcg ctgtcgctgc aaatcagtgc tccgtccggg ttgtaaaatg
     2401 caattctaag agggtctttc gagatgtcca cgatgactgc aggcgtcgaa atggccactg
     2461 attcgggagt gtcctcgatg ctcacttggg gattaggcca tnnnnnnnnn nggatcctgg
     2521 aagccgggga tgggctggtt caagcaaagt catgctcact cgcacgaggt cgtcggcgat
     2581 gacagataca tccacgcgcg aatccagggt ttccatagag acttgattgt cgccaatgtg
     2641 caggttgaga acccggccga gtggggtgaa acctttgtgt gacattttgt gatcctccat
     2701 gcaaaatctg tggcattaga gcgttttggc taatgacatt tgcgtagttc tagaccgatc
     2761 tccaataccc tcccgggtgt gcgagtgggc cagtgtccaa gtatggggtt gattggagcg
     2821 atgacggact acaaggtagt gaaggggtgt atcagcggcc aaacagggag agggcatagc
     2881 aggcaaagtt gtgcgtttta acggggcctc tattgccctt ccttgcggcg gagatcgcat
     2941 tcggaatttg attagactat gaggtcgatc tgctccatgg tgccgacggt cttgttttgg
     3001 cggacataaa gtcccatgcg ggccaactct cctacagggc ggttggcgct gtttttcatc
     3061 tggaattggg cgcccacgga accaagatat atggctccga ttcctgcttc ggctaaggat
     3121 acgaggggtt catcgcctgg attgcctcca gtccagatat acaactgggt ccaggccggg
     3181 tcgcgttcat cgagccatct gttgccgtcc gagtcgagct cggccagttc attcatgccg
     3241 tttccggtcg acgggccgaa tagctcggtt ccgtcgttta ctatgccatc gccgtttctg
     3301 tcgagaacaa ggaaaccgct gcctcggtgg agtcccggga tcatctctgt cttgccgtcc
     3361 gcattcaaat cgaactcgaa cagctcatcg gagagttggg cggcttcgcc gtcgagattt
     3421 acgatgatgg gatcgatttt tctgccattt tggatatgaa tcgacgttgc gctgtatgct
     3481 gattcttcgt ggcgcatcga aaggctaagg gagaacttta tttgctcgcc gtcctcagtg
     3541 gttatccagc cctctgcttc gaaggacatg tactggaaat cgtggtatga atactccctg
     3601 tcatactcaa tgctccattc gggctccggc atggactgag gagtctgagt ttggcggctt
     3661 cttactactt cggaaagctc gattgtctca atcttgatac ctgttaacag ttctatgatc
     3721 cgcttaattg tgcggatctt cggatcgtta gaggcttcgt ccgtgggaat ccctttcagc
     3781 gcctgcgact tgtcggttga catggagttg tctgctcgag ccagagcggc gcgggcacta
     3841 tcggatatgc tgacatggtc acgaaccaaa ctggttgctg ccgatgggcg ggtttcgggg
     3901 cctatccgca cccgaacgct ctccgacacg ctctcttgtc gcattcgcgc ataccgggaa
     3961 gacatcgata tcgtcgagtc tcttaccaac actatggtac accccctaat gcgcgtgaaa
     4021 gatatcttta tcatgttatc ggcatgttgc gccgtgagct tagctctgta gaacttgcgg
     4081 gccaaaagcg cgtttcggtt caggacagac acgcgagggc atcaattatt gccagtaacc
     4141 gtcagatttg ctgctctcag cctgttgggc tgcatgttct ctgttccgag gccggactgc
     4201 aactagtcaa atgtggtccg tcttcgagtg cagggcctat ttgaatatgg cttttctctg
     4261 tgctatactg agctagtcaa ggacaactgt gaggtctatg cctcggaggt gaagaggttg
     4321 aaggacaata tccatcctaa gttctatatt acgactgtga cctgcgcttg cggagagagc
     4381 tttgagactg ggtctacccg cgagaacatg agggttgata tttgctcaaa gtgccatccg
     4441 ttttccaccg gcgtgcagag ggtcgtcgat accggcggtc gggttgagaa gttccgcaag
     4501 aagcataatc tttagccagt tttgcttggg ggcggcttca gtatatgggg ccacccccaa
     4561 gaggcatgta cagtcggagc cctgccgggc tccggttttt tgagtctccc tgcgaggagg
     4621 gtatgctttg caaggaagca tggtgggtac aggctggatt gaagtgatat gcggctccat
     4681 gttcagcggc aagactgaag agctaattcg tagagtgaag cgtgcaagga tagctaggca
     4741 gaacgtagtg gctgtcaaac ccgctttgga tattcggttt tcatcgaaag atgtggnnnn
     4801 nnnnnnatgt ggtgtcgcac gatggcgaca agatcggatg cttgcctgtt aaggattctg
     4861 acgaggctga agctatcatc cccgatgatg tgcaggttgt ggctttcgac gaggcgcagt
     4921 ttttcgattc tggcattgtc gatctatgtg tgcgactcgc cgacagaggt gtgcgcgtta
     4981 tcgtagcggg gctagactgc gatttcagag gcgaaccctt cggctccatg ccggagcttc
     5041 tggcgcgcgc ggagtatgtg acgaaacttt cagcaatatg tgtcaaatgc ggtaaccctg
     5101 caaccagaac tcagaggatc atcgacggca ggcccgcgaa ctactcggat ccacaggtgg
     5161 tcataggggc cagcgagatg tatgaggcaa ggtgccgcag gtgtcaccaa gtgccaggga
     5221 aacctactcg gaaccggaca ggaggtaacc aaggatgaat cctcgttacc agtatggagg
     5281 ccaggctgtc atcgagggcg tgatgatgcg aggccgtgac agcgcggcga ttgccgtccg
     5341 tcgccctgac ggcgacatat cggtaaagat gcgcgatatt gatagttcca agcgcccagc
     5401 tatactcaaa gctccgttca tacgcggagt agtgacgctc atcgaatccc tcgttgtggg
     5461 aatggagtca ctgatgtact cggccaatga gtcatcagaa gaggaagagc agctgtcagg
     5521 gggagaagtc gtcctcagcc tagtaatggc gttcgggctc ttcatccttc ttttcatagt
     5581 gcttcccaat gtcattgtcg cttttatgca aaaacacata cccagcgttg tgttggtcaa
     5641 cctgttagaa ggagtcctca ggattgcgat attcctggtc tatattgtgg ccatttcgaa
     5701 aatggaggac atacagcgtg tttttgcata ccatggcgca gagcataaga ctattgctgc
     5761 atgggaggca ggcgaggagc tgactgtgga taacgcacgg aagtacagta cgctccatcc
     5821 gaggtgtggg acgagttttc tgctcatcgt catggtcttg tccatcctcg tatacagctt
     5881 tcttggcgag cagaccctag gcgaacgaat actgagtcgg ctgattctct tgccagtgct
     5941 tgcgggacta tcatacgaag ttcttaagct ggcaggcaga cccaacccgt ctgtgttcgt
     6001 taagtggttg tccgctcccg gcttggcgtt gcagaagttg accactcgcg agccggacga
     6061 ctccatgttg gaagtggcga tagcttcgct aaatgccgtg atagaaaaag atcaggcctc
     6121 aagtgaggcg gcggtgatat aagatgatag gaaagctcga tgaaatagag gcgagatacg
     6181 tggaattgga gcagcagatc gctgatccgg cggtcattgc tgatcagatc acgtatcgta
     6241 aactcgctaa ggcccattca gatctcgagg agatagtggg caaataccga aaactcaagg
     6301 acgtaatggc ggagatcgtg tctgtcaagg aaatgctcga cgggcatttg gaaccggata
     6361 tgcgcgagct cgccgaggcg gagttggatg aactcgagtc gaggcgaggg gcgctcgaaa
     6421 gcgagctccg tgttcttctt ctcccatctg atcccagaga cgaaaaaaac gtcatcgtgg
     6481 agatacgggc gggaacaggg ggagacgagg cagcgctctt tgttggcgac ctgttccgta
     6541 tgtacgcaag gtatgctgag cgccatgggt gggagttgga gagcatggct tccagtccca
     6601 ccgaacttgg aggcttcaaa gagatcatat tcatgatttc cggggacaag gtctacagtc
     6661 ggctcaaata cgaaagtggc gtccacagag tgcagcgtgt gcctgagact gaagcgtctg
     6721 gccgcataca cacttctgcg gccactgtag cggttctgcc ggaggccgag gaagtcgacg
     6781 tggagatcga tccgaacgat ctgaggatcg atgtgttcag atcatcgggg cacggcgggc
     6841 agagcgtcaa tactaccgac tctgccgtga ggattacgca cattcccacg ggcatggttg
     6901 ttacatgcca ggacgaaaag tcgcagcata agaatagaga caaggctctc agagtcttga
     6961 gggccaggct tcttgagcag cttacagagg aacaagaggc cgacatagcc gaaacgcgca
     7021 aaagtcaggt agggtcggga gatagaagcg aacggatcag aacctacaac ttccc
//
LOCUS       DAOSAI010000082         6926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7035_length_6926_cov_3.710522, whole genome shotgun sequence.
ACCESSION   DAOSAI010000082 DAOSAI010000000
VERSION     DAOSAI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7035_length_6926_cov_3.710522"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLM83_04180"
     CDS             complement(<1..945)
                     /locus_tag="PLM83_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOK70638.1"
                     /translation="MKRHTRWLLVVTVLVAVAALGIGWSAMANERSAVALDPEQWAPA
                     WQEGAEEFVPGEYLVRFVPGVQTVEAENIMGRSTMSIIKEIKFDPCKAFPEGLTLYHV
                     KVEPGVVSAEAVCALEADPRVEYVEPNRKIFAAETVIPDDEYFNLLWGLHNIGQTFIG
                     NVSGVEDADIDAPEAWAHRREAPEVIVAVLDADIQVDHPDLAPRMFINEGEIPGNGID
                     DDGNGFIDDVCGWDFHHNDNTLYDGESHHGTHTCGTIGAVGNNGIGVVGVAWNVKILP
                     IKFIGPSGGSLADGAAALAYARQMGATITSNSWGYSGGP"
     gene            complement(1548..2273)
                     /locus_tag="PLM83_04185"
     CDS             complement(1548..2273)
                     /locus_tag="PLM83_04185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOK70639.1"
                     /translation="MIKTVMFDLDGTLAHFEFEEFFRAYIEKIVESLSDVMEPKALTE
                     ALMASVEAMVSCEDPALTNRDVFVVDFFGRTGRSEAELMPLFDAFYLAEDGFPSIKQM
                     LGVTPHPSARGMIENLLDRGYEVVIATNPLFPLVAIEERMRWGDVDGLPYALVTSYET
                     SHFCKPNLKYYEEILSVLDRSPEECLMVGNDTWEDLVAGDLGITTYLVEDYILDRDPV
                     RRDPDFRGSFDDLVEFLSAEEFL"
     gene            complement(2389..3417)
                     /locus_tag="PLM83_04190"
     CDS             complement(2389..3417)
                     /locus_tag="PLM83_04190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK70640.1"
                     /translation="MTAKEIKLVAAIMLTLLAFSAGVGAEEAQTTFLVVTLKDGSSVV
                     YNIPVDGDKIDSAALAFSDVGLHELEGKHNLVALINNEPITTGAFYDEISNVVGYQAL
                     SYIIDETLVRQAAKSAGITVTQAEVDREFEMVREATGSDFDAVLAQYGMTQESFRKSL
                     EMSLLVFKVSTKDVKVSAEDIAEHYNSHIADYTIPEQVRASHILVGAEKEAEEILQLL
                     ESGADFGELAKERSEDTYSAKAGGDLGYFPRGAMVPEFERAAFSTPVGETSGIVKSQY
                     GYHILQVTGKMDARTIPMEDVWESIEWAIKSKYALDFKQLVAELKRDSAIIVFDPRFA
                     DLGTTTLP"
     gene            complement(3414..4370)
                     /locus_tag="PLM83_04195"
     CDS             complement(3414..4370)
                     /locus_tag="PLM83_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70641.1"
                     /translation="MLFVDGLERLGRDEKIWLMAQINALTSANLVMDGVEKSARAVKD
                     IAVDQVARATRRALPSRSPRIEEWRQDFDWDPEVINRKVAAEIEALSELADAQLDSEL
                     HLQLRRIANVNSEADCDEIAEGVILRAARSLGISAEEYGSLEDVEAAVFVACVAKQID
                     LLLELVGEMQADEAESAAAKLGEELESLAPDEIAAVCEAIGIESISEEAVAAGLRFCR
                     AVPMAQMVCDDFGYASTKFLVSLCKAVGFALGCAFLPGVIGGVDRLMACALSPFIAVL
                     SGRAALATSDRMLYDELARFLIVIGHGVVIEDRRNDWRQALS"
     gene            complement(4401..4859)
                     /locus_tag="PLM83_04200"
     CDS             complement(4401..4859)
                     /locus_tag="PLM83_04200"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOK70642.1"
                     /translation="MRRNCDMGGSRGSAWIWWALALAYPAFLLIASVRPEFGAEATRA
                     FIEKILPHLDPSQVHVVVVAFRKACHFLGYGFFAIVLANAIHTLRKGVRHRKWAVASC
                     IIAALIALGVAVLDEQIQSVVPHRTGASRDVALDFLGIVVGMLVKVGRGF"
     gene            4969..5415
                     /locus_tag="PLM83_04205"
     CDS             4969..5415
                     /locus_tag="PLM83_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70643.1"
                     /translation="MSYTGEEMNGRVLLLLTSATVILILTCASGSALCAPQLESLHAS
                     WIVVDGINLGCRMRITDTWRFALNFDMCHNSAYTTGSAICMYKNPIFVLTAGYVGAGI
                     SYELQSSRFYPNVLAGAEFGAAFAEYEWMPSPKNYGKLRCGMRVSF"
     gene            complement(5460..6692)
                     /locus_tag="PLM83_04210"
     CDS             complement(5460..6692)
                     /locus_tag="PLM83_04210"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOK70644.1"
                     /translation="MGRTLCPPDVKQRASGAKAVLVWAGLLALIFAALARPAGSLAAS
                     LDASGDVRIYLVDPSDAEPAFAIEHDGLELIAEFGSDENSLFVGAGSLRWGPGRSGSL
                     MMSGRAEPMPLVGYHLSRGNSDYVRFIGLMESDEARRLIGHRLEYTPTPDVVLGVSET
                     AVVSQNASRALYLPFPGFPLYALQRVVSQQDRAQDSLININLGADFSLFWDLMSGDFA
                     KWGGFAPRMELYGEVFIDDAQGHIPNRAYVPDMVGGLVGIDLPKLPWNLRFGANIEYV
                     AIANFVYSHRNPNNDYVYRGVMLGHPLGPDSDMLVLTLSYCPDKCAKLTLSGAVERHG
                     EGRAGQSWSHQHEPEDWFLSGVVETCARAVFGIERELAPMARLMGSLEYATKSNSGNV
                     LGQSSDEWNMRIGVGVKL"
     gene            complement(6694..>6926)
                     /locus_tag="PLM83_04215"
     CDS             complement(6694..>6926)
                     /locus_tag="PLM83_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70645.1"
                     /translation="FGLPQHETFDACGRNPLNLVARKKGYLRVELVFVNMLDGSGRLR
                     GKTPDELKYTNTVMQRENPDVMEAPEDIQRGD"
BASE COUNT         1661 a         2162 c         1555 g         1548 t
ORIGIN      
        1 cggaccgccc gagtatcccc acgagttgct cgttattgtg gcgcccatct gcctggcata
       61 agcgagcgca gctgcaccgt cagccaagct gccgccgcta gggcctatga acttgatagg
      121 cagaatcttc acattccatg ccacaccaac aacgcctatt ccgttgttcc ccacagcccc
      181 gatagtaccg catgtatgtg ttccgtggtg agattcccca tcatacaacg tgttgtcgtt
      241 gtggtggaag tcccagccac atacgtcgtc aatgaaccca ttgccatcgt catcgatgcc
      301 gtttcccgga atctctcctt cgttgatgaa catgcgagga gcaaggtcgg gatggtccac
      361 ctgtatgtcg gcatccaaga ctgcgactat tacctccggc gcttcccgcc tgtgcgccca
      421 ggcctcaggc gcatctatgt ctgcatcttc aaccccggac acatttccga taaaggtttg
      481 tccgatatta tgaaggcccc acagaagatt gaagtattca tcatcaggaa tcacagtctc
      541 agcagcaaat atctttctgt taggctccac atattccacc ctaggatctg cttccagcgc
      601 acataccgcc tccgcagata ccacaccagg ctctactttt acgtgataga gggtaagccc
      661 ttccgggaat gctttgcagg gatcaaattt gatctccttg ataatactca tagtcgatct
      721 gcccatgatg ttctctgctt ctacagtttg caccccggga acaaatctca ccaagtattc
      781 gcctggcacg aactcctccg ccccctcttg ccacgcaggg gcccattgct caggatccag
      841 cgcgaccgcg gaacgttcgt ttgccatggc tgaccagcca atgccgaggg ctgccactgc
      901 aaccaacaca gtgacgacta acagccatcg agtatgtcgc ttcattgcaa aatcgcatcc
      961 tcctctattt cgatgtcaca tacgataaaa aatacatcta cccagcaacc acagatcagc
     1021 atctattccc catgttttgc aggaaagcat cgctccctcc aatccgttca actctcattg
     1081 agttttcact caacaaacgg gcgttccatg aatctgtctg cttgataata taaagaacag
     1141 gtcactcgcc acagtgccct cagcggccct ctggaagtga cccgcgatac gtgcctgaat
     1201 ggcccgcatg gcgtctagat ctcgttgatg ccttgtcaca cataaaaaac tctgttgcaa
     1261 gacgccaggc tcaaaaaagc agagcccttt tgctcaagaa gatgcgttcg tttggatacc
     1321 aagctggacg gccaatccag gcaagctgga taacgcggtc agcgcaactg tcttcgctgc
     1381 tactgatctt gccactacca atgccacaaa ccaagcgcac attcactaga cagactccgg
     1441 tctcactaat gtttctacct ggggcggcga attccttcca tttgtgcatg gattggtaaa
     1501 actgccaggc cgtttaggca attatggcgc cccgagcaac acatgcacta caggaactcc
     1561 tccgccgaga ggaactccac aagatcgtca aaactgcccc tgaagtcagg atctcgcctt
     1621 acgggatcac gatctaagat gtagtcctca accaggtaag tcgtgatccc aagatctcct
     1681 gccacaagat cctcccaggt atcgttccct accatcaagc attcttcagg ggatctgtcc
     1741 aaaacagaga gtatctcttc gtaatatttc agattaggtt tgcagaaatg tgaagtctca
     1801 tacgatgtca caagagcata aggaagaccg tccacgtcgc cccaacgcat cctctcttca
     1861 atggctacca gaggaaagag cggatttgta gcgatcacaa cttcgtatcc cctgtcaagc
     1921 aggttttcta tcatgcctct ggcagaaggg tgcggcgtga cgcctagcat ctgcttaatg
     1981 ctgggaaacc cgtcttcagc aaggtaaaaa gcatcgaaca gcggcatcag ctcggcctcc
     2041 gatcgccctg tcctgccgaa aaagtcgacc acaaagacat cccgatttgt aagggcggga
     2101 tcttcacacg agaccatcgc ctcgaccgat gccatcagag cctctgtaag agccttcggc
     2161 tccataacat ctgacaggct ctctacgatc ttctctatat acgccctgaa aaactcctcg
     2221 aactcgaaat gtgcaagagt gccatcgagg tcgaacataa cagtcttaat catgtaatca
     2281 cctcggatat gaaagctagt gtacggcctc cggcacatgg ctagacgatt cgccctatgc
     2341 agccccagcc ttgccaaggc cacggcagca cccaactgag taaaatgtct atggcaacgt
     2401 cgtcgttcca agatctgcga aacggggatc gaacacgatt atggcgctgt cacgcttcag
     2461 ctctgcaacg agctgcttga aatcgagagc gtatttgctc ttgatcgccc actcaatgct
     2521 ctcccaaaca tcctccatgg gaatggttct ggcatccatc ttcccagtca cctggagaat
     2581 atggtatcca tactgtgact tcacaattcc gcttgtctcc ccgaccggag tcgagaatgc
     2641 ggcccgttca aactcgggta ccatggcccc ccgggggaaa tagcccaggt caccgccggc
     2701 cttcgccgaa tacgtgtcct cagacctttc cttggcaagt tccccgaagt ccgcccccga
     2761 ctcgagcaac tgcaggatct cttctgcttc cttctccgcg cctacaagga tgtggctcgc
     2821 cctcacttgc tccggaatcg tgtagtccgc aatgtgagag ttgtaatgtt cggcaatatc
     2881 ctctgccgac accttcacat ccttcgtaga gaccttgaat accagaagac tcatctctag
     2941 actcttgcga aaactctctt gcgtcatgcc gtattgggct agaacagcgt cgaagtcaga
     3001 acccgtcgcc tcgcgcacca tctcgaactc acgatctacc tcggcctgtg tcaccgtaat
     3061 ccccgcgctc ttggctgcct gcctgacaag ggtctcatca atgatgtagg aaagcgcctg
     3121 atagcccacg acgttcgata tctcatcata aaaagcgcct gtcgtgatcg gctcattatt
     3181 gatcaaggca acaagattgt gcttcccctc gagctcatga agcccgacat cggaaaaagc
     3241 gagtgccgcc gagtctatct tgtcgccgtc aacaggtatg ttgtagacca cagatgagcc
     3301 atccttcagc gtcaccacaa gaaaggtcgt ttgagcttcc tcagcgccaa ccccggctga
     3361 aaaagcaaga agtgtaagca taatcgccgc gaccagttta atttcctttg ctgtcatgat
     3421 aatgcctgcc tccaatcgtt ccttcggtcc tctataacca caccgtgtcc tataacaatc
     3481 aaaaaccgag ccaactcgtc gtagagcatt cgatcggacg tggctagcgc tgccctgccc
     3541 gagagaacgg ctataaacgg cgacaacgcg cacgccatta acctatcgac tccgccaatt
     3601 acccccggca agaacgcgca tcccagagca aacccaaccg ccttgcagag gctcacaagg
     3661 aacttcgtag atgcgtaacc gaaatcatcg cacaccatct gcgccattgg cacagcccgg
     3721 cagaacctga ggcccgccgc aaccgcctcc tcagaaatgg actcgatccc tattgcctca
     3781 cacaccgccg cgatttcatc cggcgccaga ctttccagtt cctcgccgag cttggctgct
     3841 gctgattccg cctcatctgc ctgcatctcg ccaactagtt caagcaggag atctatctgc
     3901 ttcgccacgc aggcgacaaa taccgcggcc tccacatcct ccaaactccc atactcctcg
     3961 gcactaatcc ccaggctacg tgctgccctc agtatgacac cttccgctat ttcatcgcaa
     4021 tcagcctcgc tattcacgtt ggctatgcgc cgcaattgga gatgaagctc cgagtcaagc
     4081 tgtgcatcgg caagctccga cagcgcctca atctccgccg caaccttgcg gttgattacc
     4141 tcaggatccc aatcgaagtc ctgccgccac tcctctattc tcggagaccg cgatggcaag
     4201 gcacgcctag ttgcacgcgc aacctgatcg acagcgatgt ctttcacggc tctggcgctt
     4261 ttctcaacac cgtccattac aaggttcgcg cttgtcaagg cgttgatctg agccatcagc
     4321 cagatcttct catccctacc tagacgctct aaaccgtcaa caaaaagcat gaccacgtcc
     4381 cccaaagcgc ctttggaaga tcaaaacccc ctgcccacct tgaccaacat tccaacgacg
     4441 attcccagaa aatcaagggc aacatcccta ctcgcgcccg tcctatgggg aacgaccgat
     4501 tgaatctgtt catccagtac agccactccc aaggcgatca gagccgcgat tatacaggag
     4561 gcaaccgccc atttcctatg gcgcacgcct tttcgcagag tatggatggc attagccagc
     4621 acaatagcga aaaatccgta tccaagaaag tgacatgcct tgcgaaaagc caccacaacc
     4681 acgtgcacct ggcttggatc gaggtgtggc agtattttct ctatgaatgc gcgggttgct
     4741 tcagcgccga actcaggccg caccgatgca ataagcagga acgcgggata tgccaacgcc
     4801 aaggcccacc atatccacgc cgagcctcgg gaacctccca tatcgcaatt tcgcctcaat
     4861 ccaatatcct ccatccgttg ccgaactgcc gatcaggagg attcctggta cattcggcta
     4921 attacgcagt aagatgatat cccctgatat ggggatgcat tgaacattat gtcatataca
     4981 ggtgaggaaa tgaatggacg agttcttctt ttattgacat cagccacagt catcctcata
     5041 ttgacgtgcg catccggatc cgctctatgc gccccgcaac tcgagtcgct acacgcatcg
     5101 tggatagtgg ttgacggcat aaacctcggt tgcagaatgc gcattacgga tacatggcga
     5161 tttgctctta atttcgacat gtgccacaat agcgcctaca ccactggttc cgcaatctgc
     5221 atgtacaaaa accccatctt cgtgctgacc gccgggtatg ttggggcagg gatcagctac
     5281 gaactccaaa gcagccgatt ttatccgaac gtattggccg gcgcggagtt cggagccgca
     5341 ttcgccgaat acgagtggat gccgtctccg aaaaactacg gaaaactcag atgcggcatg
     5401 agggtatcgt tctagggggg cggaccaccc gccccttttc ccttgcacgg atgggccccc
     5461 taaagcttga cccccacccc tatcctcata ttccactcgt cagacgactg tcccaataca
     5521 ttgccgctgt tggatttggt cgcatattct aagctgccca taagacgcgc cataggggcg
     5581 agttctcgct ctatcccaaa cacagccctg gcacaggtct ccacaacccc tgaaagaaac
     5641 cagtcctccg gctcgtgctg gtgcgaccat gactgccctg ccctgccctc tccgtgcctt
     5701 tccaccgcac ccgaaagcgt cagtttcgca catttatcgg gacagtaaga gagagtgaga
     5761 acaagcatgt ccgaatcagg ccccaaagga tgccctagca tgacgcctcg atatacataa
     5821 tcgttgttgg ggtttctgtg cgaatagacg aaatttgcta tcgctacata ctcgatgttg
     5881 gcgccgaacc gcaagttcca tggcagcttg ggaaggtcga tgccgaccaa cccgccgacc
     5941 atgtcgggaa cgtaggctcg gttgggtatg tgcccctgcg catcgtcgat gaagacctcg
     6001 ccgtacaatt ccatgcgggg cgcaaagccg ccccatttcg caaaatcgcc cgacatcagg
     6061 tcccaaaaca gactgaagtc ggcccccaag ttgatattga tcaggctatc ctgggcccgg
     6121 tcctgctggc tcaccacacg ctgtaatgca tacagtggaa acccgggaaa cggcagataa
     6181 agcgccctcg acgcgttctg cgagaccaca gcggtttcac ttactcccag cacaacatcg
     6241 ggggtggggg tgtattccag tctatgacct ataagccttc ttgcctcatc ggattccatg
     6301 agtccgatga acctaacata gtcggaattg cctctgctga ggtgataccc cactaaaggc
     6361 ataggctccg cccttcctga catcatcaaa gaccctgatc tgcccggccc ccatctgagc
     6421 gagcctgccc ccacaaacag gctgttctca tcggaaccga actcggcaat gagctcaagt
     6481 ccatcatgct cgattgcaaa agccggctct gcgtcagatg gatcaacaag atatatacgg
     6541 acatcgccac ttgcatcgag cgaagccgca agactccctg cgggccgcgc cagtgccgca
     6601 aaaatcagtg ctagtagtcc cgcccacacc aacacggcct tcgccccgct cgccctctgt
     6661 ttcacgtccg gtggacatag cgttcttccc atatcaatcc ccccgctgta tatcctctgg
     6721 agcttccatt acatcgggat tttcccgttg catcactgtg tttgtatact tcaactcgtc
     6781 tggcgtcttc cctctcaatc ttcctgaccc atccaacata ttcacaaata ccagctcaac
     6841 ccggagatat ccttttttac gggcaaccag gttcaaagga tttcgcccac atgcgtcgaa
     6901 ggtttcgtgt tgaggcaggc cgaagg
//
LOCUS       DAOSAI010000083         6786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7219_length_6786_cov_3.202199, whole genome shotgun sequence.
ACCESSION   DAOSAI010000083 DAOSAI010000000
VERSION     DAOSAI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7219_length_6786_cov_3.202199"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLM83_04220"
     CDS             complement(<1..312)
                     /locus_tag="PLM83_04220"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70646.1"
                     /translation="MRADLEQAIRAGRIAPIYILYGEDSYSRQVNADELAQAIAAARG
                     GADLNVYDMSESSVDDIVTAILTVPMFASSRVVVVRRFEEISAAQQGRIISLIAPGKD
                     RA"
     gene            complement(383..1216)
                     /locus_tag="PLM83_04225"
     CDS             complement(383..1216)
                     /locus_tag="PLM83_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019154132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK70647.1"
                     /translation="MSKRRKTVGMSIVYILLTVGAVIMLLPFYYMLITSVKGPEELYT
                     PELVWFPKRFVWSNYSEAWNADPNFPRYFFNSAFIATITTALQLVTSALAAYAFAMID
                     FAGKETVFMILLGTMMIPSQVTLVPNYAIIAKAGWIDTYKALIVPFAASAFGIFMMRQ
                     FFQTIPMELWDAAQIDGCSRFEYLWRVIVPLSKPVFVTNGLFTFIGGWNSFLWPLIVT
                     NSSTYRTIQVGLSQFNQEFGTVPNLLMAASTIAIVPLVIIFFIAQNQLIEGIARTGLK
                     G"
     gene            complement(1233..2135)
                     /locus_tag="PLM83_04230"
     CDS             complement(1233..2135)
                     /locus_tag="PLM83_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK70648.1"
                     /translation="MNSLERKRYVKDTVAAYMYLAPALIVIIVFNLFPAFYALYISLF
                     DTNLIGRWDWVGLGNYSELFRDADFLQSLVNTGKYVLGTVPVGMALALLIAWLLNKPI
                     RGLSAYRTAYFLPYVTPIVAISIVWTWIYKEDSAGMLNSLLCLFGAEPQAWLLDPKWS
                     MFALCLMSVWRHLGYNVVLFLAGLQNIPAEYYEAAEIDGASGWSMFAHITWPLLSPTT
                     YFILMVSIIGSFQVFTQVYVLWPSVSGGPLGTTRVVVKYLYDQGWGAFRFGYASAIGY
                     ILFAIIFVLTLVQKDIVGKRVHYQ"
     gene            complement(2135..3091)
                     /locus_tag="PLM83_04235"
     CDS             complement(2135..3091)
                     /locus_tag="PLM83_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOK70649.1"
                     /translation="MNRNDVPIHGATVLLYEMREGAPTFISSAQVDLPAGRGMPVSFE
                     WTPSKAGDVSFVLELVACGEGARDIHCGKSARVRSSDIKSVLIDEGHNNRCSGYMDAF
                     ARLLGEADYNPRINEGRIDAQTLDGADILVINVPETGFSLTPTSFDDEELDVISSFVA
                     AGGSLLLAGGSDFPGLRSCDQFDLLLEKMGSAIRFNRDEMRRDDLSVLDVEWGLERAV
                     YADEACSLAGPGWIDLSEVPQVVVLAHAPGAWSADVSGEGRLFQYAGSPVFAAAETVG
                     SGRLVCLGAPVYSSRDLARERCGNGAFVLSIISWLAGGEWNR"
     gene            complement(3125..4258)
                     /locus_tag="PLM83_04240"
     CDS             complement(3125..4258)
                     /locus_tag="PLM83_04240"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOK70650.1"
                     /translation="MHKQPISRRRLVLVWATLFSVMAGCVCLGAEPVVELNCYFGNLH
                     SHTSYSDGVLTPSDAFRHARDVAMMDFLAVTDHGYYLQEATNIHHWFKSLEEADEVYQ
                     PGVFVSMIGFEWTFTDGHMNGLDTPLAASRDTQRNLAAFIDFLAHYNGIGVFNHPNYD
                     IQPNWNDFEYLGEGDRFVCLLEVGSGPYRHNIRNERAYCRALDRGWHVGAISCQDNHK
                     ADWGTAAPTRTAVLASELTREAIYESLRSMRTYATEDSNVRVSFTSGDCQMGSDIIIS
                     RDDIDSGRPVYFEIVIDDPDEDDMLSSVEIITNGGVVVWEREVCGTGVFRADVEIMPA
                     QSYNWYYVRAVQADMDVIVTSPIWVSTGSQIAVSDLGCVDLAP"
     gene            complement(4419..5684)
                     /locus_tag="PLM83_04245"
     CDS             complement(4419..5684)
                     /locus_tag="PLM83_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK70651.1"
                     /translation="MKRFATIALMAAIILATTAVGPSMAAGKTTITFWHAMGAQLGKT
                     LEGLVEEFNRQSPDVYVKAEYQGNYGALSQKIVGALVARKPPTMAQVYGNWAAEYISG
                     NELVPIQKFVKGPNGLSQLEVDDIWDGLREGCMFDGVWYTMPFNKSVYVLVYNQTALK
                     EAGISGPPKNWQELVNTAKALTVKDGDKIVRYGFGLRPNIDIFACFFFNAGGSWLNPQ
                     GKVAFNSPAGVTALQFMADMLNKYKVSYYVPGYLDADFGAGKVAMYFTTSPGLSYTEN
                     SVAGKFEWSAAPLPYMDPKFRSTPVAGTDLAIFSRATPEEQEAAWKFIKWLVEPRQTA
                     KWAIGTSYIPVRKSAMHLDMMKKWFAVHPRDEQSLQQLRYVKYDPNMAAWNNIRGDVS
                     EAVEKVFLGKATPKEALDAAAAKANSRIR"
     gene            complement(5794..>6786)
                     /locus_tag="PLM83_04250"
     CDS             complement(5794..>6786)
                     /locus_tag="PLM83_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK70652.1"
                     /translation="LSRICRNKLGIVLTIAALILGVVIAWPRPLEVVFLSVGQGDAAV
                     LFTPEGHTLLIDAGTERAGERHVLPYLERCGKRHIDAAIITHEHLDHAGGFAALAGKV
                     QTDTVMIPRGMDQAWIDGILVGSECQYSNAPSLAQLVRGNQINAGSVRLEMINPIVYG
                     GGPEAVGDGLRGTSLEGDVGFSGNLTFDPNEASLVFHVVCGEFSMLLCADAGRKFEQE
                     VFRCLGPEGHGVLRADVLKVGHHGSSSATSARFLEAVKPSIAVISVGRNSYGHPSQDT
                     VERLLQSGAAVLRTDEVGAVRVEWHPKSHRLAVSDMRALWQTAPMFRKLETLDK"
BASE COUNT         1698 a         2033 c         1582 g         1473 t
ORIGIN      
        1 cgcgcgatcc ttgccaggag cgataaggga gattatcctg ccctgctggg ccgcggaaat
       61 ctcttcgaat ctgcggacca ccactactct ggatgacgca aacattggaa cagtgagtat
      121 ggctgtgacg atgtcatcca cagatgactc cgacatatcg tagacgttga gatctgcgcc
      181 tccccgggca gccgcaatag cttgcgcgag ctcatctgca ttgacctgcc gggagtatga
      241 gtcttcaccg tatagtatgt atatgggcgc aattcgccca gccctgatcg cctgctcaag
      301 atctgctctc aatgatatcc ccctcatact ccttgcaaat cgcccagtcg aaaccggaat
      361 ctgatccgtc cccgccgcct tgctaaccct ttaagcctgt gcgcgctatc ccctcgatca
      421 gctggttctg ggctatgaaa aagattatca ccaatggcac tatggcaatc gtagaggcgg
      481 ccatgagcaa gttgggaaca gttccaaact cctggttgaa ctgagaaagc ccaacctgta
      541 tcgttcgata tgtcgaagaa ttggtgacaa tcagaggcca cagaaacgaa ttccacccgc
      601 cgataaacgt aaacagcccg tttgttacaa atactggctt cgacagggga actatcaccc
      661 gccacagata ctcgaatctg ctgcaaccgt cgatttgggc agcatcccac aactccatag
      721 ggatggtctg aaagaattgc ctcatcatga atatcccaaa agcgctcgca gcgaacggga
      781 caataagggc tttgtatgtg tcgatccagc ccgccttggc tattatcgcg tagttcggaa
      841 ccaacgttac ctggctcggg atcatcattg tgccgagtag aatcatgaac acagtttcct
      901 tgcctgcaaa atcgatcatt gcaaatgcat aagccgcaag agccgacgtg actagctgca
      961 aagctgttgt aatagtggca atgaacgcgg agttgaagaa ataccgcgga aaattgggat
     1021 cagcattcca tgcttcagag taattgctcc atacaaacct cttgggaaac cagacaagct
     1081 caggcgtata caactcctcc gggcccttca ccgaagttat cagcatgtag tagaagggga
     1141 gaagcattat caccgctccc acagtcagga ggatatatac gatcgacatt ccaacagttt
     1201 ttctacgttt cgacaaacta atctcccccc gactattggt agtgcacgcg cttgcccaca
     1261 atatcttttt ggacaagcgt caacacaaag atgatagcga aaagaatgta cccgatcgcc
     1321 gatgcatacc caaacctaaa agccccccaa ccctggtcgt aaaggtattt gacgacgacc
     1381 cgagttgttc cgagcggtcc gcccgataca gatggccata acacatagac ctgggtaaag
     1441 acttggaacg atccgattat cgacaccatc aatatgaagt aggtagtagg cgacagcaac
     1501 ggccacgtta tgtgggcaaa catcgaccag ccacttgccc catctatttc cgccgcttcg
     1561 tagtactccg caggtatgtt ctgcaaaccg gccaggaaaa gcacgacgtt ataacccaag
     1621 tgcctccaga cgctcatgag gcatagagcg aacatcgacc atttgggatc gagcaaccag
     1681 gcctggggtt ctgccccaaa aaggcacagc aaggagttga gcatccccgc agagtcttcc
     1741 ttatatatcc acgtccacac aatcgaaatc gccacaatag gagtgacata gggcaggaag
     1801 taagcggtcc tgtacgcgga caatccgcgt atgggcttgt tgagcaacca tgcaatgagc
     1861 agcgccaacg ccatacccac agggacggtt ccaagcacgt atttcccggt gttcaccagg
     1921 gattgcagaa aatcagcatc acggaaaagc tccgaatagt taccaagccc cacccaatcc
     1981 catctgccta tgagattggt gtcgaacaag cttatgtaga gagcgtagaa cgcaggaaac
     2041 aagttaaata cgatgatcac tatcaacgcc ggggcaagat acatgtatgc tgcgacagta
     2101 tctttgacat atctcttcct ctccaatgaa ttcattacct gttccactct cctccggcaa
     2161 gccaagatat tatcgacaac acaaacgcac catttccgca acgttcccgg gcgagatcgc
     2221 gcgacgaata gacgggcgcc cccaggcaga ccagacggcc tgaccccaca gtttcggcag
     2281 ccgcgaaaac aggcgacccg gcgtattgaa acagccttcc ctcgccggag acatctgccg
     2341 accaagcgcc cggcgcatgc gccaacacca ctacttgggg cacctcacta agatcgatcc
     2401 agccagggcc cgccagcgag cacgcttcat cggcatatac agcacgctcc agcccccatt
     2461 ccacatccaa gacagacaga tcatcccgcc tcatctcgtc cctgttgaat ctgatggccg
     2521 atcccatttt ctccagcaac agatcaaact gatcacaact gcgcaagccg gggaaatcgg
     2581 acccgcctgc cagaagcaga gacccgcccg ccgcaacaaa actggaaatt acatcgagct
     2641 cttcatcatc aaaagacgtg ggtgtcaggg aaaacccggt ttccggcacg tttattacga
     2701 gtatgtctgc accatctaga gtctgggcgt cgatgcgtcc ttcatttatc cgcggattat
     2761 aatcggcttc gcccaggagt ctggcaaatg catccatata tcccgagcag cgattgttat
     2821 gcccttcatc tattaacacg ctcttgatgt ctgaactcct cactctggca gatttgccac
     2881 agtgaatatc gcgtgcccct tcgccgcagg caacaagctc cagaacgaaa ctgacatccc
     2941 ccgccttgct cggcgtccat tcaaaagata caggcatgcc gcgtcctgcg ggaaggtcca
     3001 cttgggcaga ggagatgaac gtgggcgctc cctcccgcat ctcatacaaa agcacagtgg
     3061 cgccgtgtat tgggacatcg tttctgttca cgatctccgc acacactcgc gcgggattcc
     3121 cctctcacgg tgcaagatca acgcagccca gatcagacac ggcaatctgt gaaccggttg
     3181 aaacccagat agggctggtg acaatcacat ccatgtccgc ctggaccgcc ctcacataat
     3241 accagttata gctttgagcc ggcattatct ccacatctgc tctgaaaacc ccggtcccac
     3301 acacttcgcg ctcccacacc acgacgccac cgtttgtgat tatctccacc gatgacaaca
     3361 tgtcatcttc atcgggatca tctatgacga tttcgaaata cacagggcgc cccgaatcta
     3421 tgtcgtcccg cgagatgatt atatcgcttc ccatctggca gtcgcctgag gtgaaggata
     3481 cgcgaacatt cgagtcctcc gtggcataag ttctcatgct tcgaagcgac tcgtatatgg
     3541 cctcccgggt caactcggag gccaagaccg ctgtacgagt gggagccgct gtcccccagt
     3601 ccgccttgtg attatcttgg cacgatatgg ctccaacatg ccatcctcga tcaagagctc
     3661 gacagtatgc ccgttcgttc cggatgttgt gtcggtacgg gcctgaacca acttccagga
     3721 gacagacgaa acgatcgcct tcaccgaggt attcgaaatc gttccagttc ggttggatat
     3781 cgtagtttgg atgattgaaa actccgattc cattgtaatg ggcaagaaag tcgatgaaag
     3841 cggccagatt ccgctgcgta tcgcgggacg cagcgagagg agtatccagc ccgttcatat
     3901 gcccatccgt gaatgtccat tcgaatccga tcatggaaac gaatacgccg ggctgataaa
     3961 cctcatccgc ttcctccaag cttttgaacc aatgatggat attcgtggct tcctgcagat
     4021 agtagccgtg atcagtaact gctaagaaat ccatcatcgc aacatccctc gcatgccgaa
     4081 atgcgtccga cggcgtgaga accccgtcgg aataggatgt atgagagtgt aggttgccaa
     4141 aatagcagtt aagctcgaca acaggctccg cccccaagca gacgcaacct gccattaccg
     4201 aaaagagcgt cgcccagacc aacaccagtc tacgcctact aataggctgc ttgtgcatga
     4261 tagacaccgc tttcacaaga tttgaagccc ggggcgcaca ggcattgcgc ccgacgcccc
     4321 aggcatccgt agaccctatt gcgaagggac aaccttgtcc gagtcgctga cgatcacaga
     4381 atactcaaat ccattgcgtc ccggctgcag cgcccgggct acctgattct tgagttcgcc
     4441 ttcgctgccg cggcatcaag agcttccttg ggtgtagcct ttcccaggaa aaccttttca
     4501 accgcttctg acacatcgcc ccttatgttg ttccatgcag ccatgttcgg gtcgtatttg
     4561 acgtaacgca gctgctgcaa actctgctca tcccttggat gcaccgcaaa ccactttttc
     4621 atcatgtcaa gatgcattgc ggacttgcgg acaggaatat agctggtgcc tattgcccat
     4681 tttgcggtct gtctgggctc gacaagccac ttgatgaatt tccacgcagc ctcctgctcc
     4741 tcaggagtgg ctcttgagaa gattgcaagg tcggtgcctg caacaggcgt ggatctgaac
     4801 ttagggtcca tgtacggcaa aggtgccgcg ctccattcaa acttgccagc aacagagttc
     4861 tcagtatagc tgagccctgg tgacgtcgtg aaatacatgg ctaccttgcc tgctccgaag
     4921 tcggcatcga gatagccagg gacatagtat gacactttgt acttattgag catgtcagcc
     4981 atgaactgaa gggctgtgac gccggcgggc gaattgaaag ctaccttccc ctgcggattg
     5041 agccatgacc cccctgcgtt aaagaagaaa caggcgaata tgtcgatgtt tggcctgaga
     5101 ccaaagccgt acctgacaat cttgtcgcca tccttcaccg tgagcgcctt ggcggtgtta
     5161 acgagttctt gccaattttt cggcggaccg gaaatcccgg cctccttcag agcggtctga
     5221 ttgtagacga gcacatacac gctcttatta aagggcatcg tataccaaac cccgtcaaac
     5281 atgcagccct ctctgaggcc atcccatata tcatccactt ctagctggga aaggccattg
     5341 ggacctttga caaacttctg aatcggaacg agttcgttgc cgcttatgta ctcagctgcc
     5401 cagtttccgt atacttgcgc catagtagga ggcttccgcg ccaccaaggc accgacgatc
     5461 ttctggctga gcgcgccgta gtttccttga tactcagcct tcacgtaaac atcaggactc
     5521 tgcctgttga actcctcgac aagtccttcc agggtcttgc ctagctgtgc ccccatagca
     5581 tgccagaaag taatcgtcgt cttcccggcc gccattgaag gaccgacagc cgtagtagca
     5641 aggataatcg cagccatcag cgcaatagtc gcaaacctct tcaatgccta ctccccgtac
     5701 tgcttcagca atcggggaac gacccccctc tctagttttg accttgcaca cctagttttc
     5761 gacgaagagg tctctactcc tgctgattta agtctattta tccaacgtct caagctttct
     5821 gaacattggc gcagtttgcc acagagccct catatcgctc acagccagcc tgtgggactt
     5881 aggatgccat tccactctca cagcgcccac ttcatcagtg cggagaacag ctgcacccga
     5941 ctgcagaagc ctttccactg tatcttgcga cggatggcca tagctatttc gccctaccga
     6001 tatcaccgca atactaggct tcacagcctc caaaaatctt gcagaagtcg cactggacga
     6061 tccgtggtga cccaccttga gcacatcggc ccttagcacg ccatgcccct ctggccccaa
     6121 acaccgaaac acttcttgtt cgaatttccg acccgcatcc gcgcacaaaa gcatggagaa
     6181 ctcgccgcac acaacgtgaa acacaaggga agcctcgttg ggatcaaacg tcaggttgcc
     6241 cgagaatcca acgtctcctt ccaacgaagt gccgcgcaac ccatcgccca ctgcttccgg
     6301 accaccaccg tacacgatgg gattgatcat ctcgagcctc acagatcccg cgttgatctg
     6361 attaccccta accaactgtg caagagacgg cgcattgcta tactggcact ccgaccctac
     6421 caatatgccg tcgatccatg cctgatccat gcctctggga atcattaccg tatccgtctg
     6481 aaccttgccc gcaagggccg caaagccgcc tgcgtgatcc aaatgctcat gtgtgatgat
     6541 agccgcatca atgtgcctct tcccacagcg ctcaagatac gggagcacat gcctttcccc
     6601 ggcacgctca gtccccgcgt ctatcagtaa cgtatgccct tccggcgtaa agagcacggc
     6661 agcatccccc tgccccactg aaagaaaaac aacctcgaga ggccttggcc acgcgatgac
     6721 cacgcccaga atcagtgcag cgatggtaag aacaatgcca agcttgttcc gacagatacg
     6781 cgatag
//
LOCUS       DAOSAI010000084         6719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7297_length_6719_cov_4.294568, whole genome shotgun sequence.
ACCESSION   DAOSAI010000084 DAOSAI010000000
VERSION     DAOSAI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7297_length_6719_cov_4.294568"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PLM83_04255"
     CDS             complement(<1..1195)
                     /locus_tag="PLM83_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOK70653.1"
                     /translation="MQPFVHLHVHSQYSFLDGADTCSTLVARAAECGMSAIAITDHNN
                     VSAAVELSKAAAKYGIKPIHGVEVSMSDGSHLTLLAQNPEGYANICRMLTEAHMSSPR
                     REPRTNMETLAKYTEGVMALSGCNRRGKIPSLVASSRFEDAKDTAENLTSIFGKENFY
                     IELEHSLTPRALSLNAALAELAQEIGVGTVATNNVHYANKPRFEVHDALTCVRTLTTL
                     DDIHPERRINAENYLKSADEMSELFAKYPEALANTNEIADKCSPGLDLSLRLFPKYQT
                     PKGLSSAEYLVRLTREGAIAQYGKITPVIENRLSHELSIITALGFEDYFLVVWDIAQW
                     AQSQGIRYAGRGSAADSAVAYCLKLTNVDSIARGLLFERFMSLERAQKPDIDIDFESE
                     RRDDVA"
     gene            complement(1269..1514)
                     /locus_tag="PLM83_04260"
     CDS             complement(1269..1514)
                     /locus_tag="PLM83_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HOK70654.1"
                     /translation="MSKRIWAQVRVIEAQNGAPAQFYWSNRWNRVTGIVDSWRESGEW
                     WDGEEEKSYYLVSLASGGCYELCREAPGQWVLSRVLD"
     gene            complement(1603..2793)
                     /locus_tag="PLM83_04265"
     CDS             complement(1603..2793)
                     /locus_tag="PLM83_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70655.1"
                     /translation="MRSIAYIHIPFLYATAHARLEPSLGGKPIAVTKKNTVIGVSPQA
                     LATGVTPGTTRRHARQVCSEIVFASYEQDSCSELANGIWDICAEYTPLVEPIAPDGAF
                     ADFTGCGAARTIANDIMHRAENEAGIIVSIGLASSKFVARAATPTIHNAGTCRCIPPE
                     AQQRFLDKLPVSSVWAIPSQVVAHLEKLGIKTLAQLRKVPMAELTLQLGEAGLMAYQL
                     SCGIDHSRVQAAYPEESIECSMSFESPVQTWADIVQCIAVCSSKIASKLRDSGRNASW
                     AEIYLEFASSHRNDYTSGKYRRRSFRSPTDSAYQIAHGLERIIEDQVSMPITAINARV
                     GGLVVPEEVQLSIFSSASTEPEKAQMDRALQYVRERFGAESVITANMLVVSRRDRMLA
                     ESFL"
     gene            3283..4302
                     /locus_tag="PLM83_04270"
     CDS             3283..4302
                     /locus_tag="PLM83_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOK70656.1"
                     /translation="MKNRVVAFALAIIAILSLTLGSSLAAPPKHKVAYIINGALGDLS
                     FYDSGQEGIDRIRKELNAETRTIECNFDPVAYPQALQSAVAWGADVIFVVSYGFEDLL
                     MQYADTYPDKKWINLDTVVENKKKTITSVDFIEEEGAFMAGVVAGLVTTDTSIPGVNR
                     DKVVGAVCGDDDPVIRGFLYGYEQGAKHIDKDIEVKAIFTGTWDDPVRGKQAAKQLYS
                     QKVDIIFQIAALTGSGVLEAAKEEGKYAIGVDSNQNGIQPGHVVTSMLKHMGQSIYRV
                     YGTILDGKYKPGMVLEYGLREGGVGLAIDEHTRRILPAKSLQRVIDIENKVKSGAIKV
                     ERYRG"
     gene            4482..6044
                     /locus_tag="PLM83_04275"
     CDS             4482..6044
                     /locus_tag="PLM83_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70657.1"
                     /translation="MQDQFIKMDSITKIYPPDTIALDDVDLVVEEGEIHSIIGENGAG
                     KSTLMKVLYGMTPPSGGEISFRGRRTRFSTPREAVAAGIGMVHQEFMLIPSYTVYENV
                     ILGAESVGRFGTLRHDDAKPKVQELVDTYGLNIDIDSRISDLSVGAQQKVEILKLLYR
                     DVDVLIMDEPTAALTPQEAGELFTRLKSLNEAGKTIIFISHKLDEVLELSDRITVMRK
                     GKRVTTVDNRGLDKAELARAMVGRDVVFSIKKTPASPEEVVFEARSLGYIDANGATRL
                     KDVSFDVRRGEIVGIAGVEGNGQYELVQLVTGLLPAQSGRVFIGGHDVTDEDVMQRRK
                     LMCYVPQDRKVSGSAQSMSLVDNAIMTHHRLNPEIEDRWKALLSSGRCRAFASRLVED
                     FGVLAPQLDAPISSLSGGNQQKVVVGREFMLDTDFILLDQPTRGLDVGSIEYVQQEVV
                     RRRDAGAGCLLISADLDELFSLSDRILVMARGRIVAELRPEATTREEIGEYMLGAKEA
                     QKAWRAGKEVGR"
     gene            6041..>6719
                     /locus_tag="PLM83_04280"
     CDS             6041..>6719
                     /locus_tag="PLM83_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70658.1"
                     /translation="MKKQLLPAIIGIVVALLAGLIILWAQGYPPLQSYAALFSYSLFS
                     RFAFFSTLDKATPLILTGLSAAVGFGSNCVNLGQPGQFLIGAMAVTAFGIAVDLPAAI
                     MIPLSILVALAAGACWSGIAAGLRRRFNMDEFITTLMLNFIADYFTLYLISGPMLDTK
                     MYSPLTPMTNSGAWLPIWNGFGSAFLVAVAAFAVVSVLWNRSKIGYEWRAMGQNPIFS
                     RTGGCRND"
BASE COUNT         1508 a         1661 c         1939 g         1611 t
ORIGIN      
        1 cagccacgtc atcgcgtctt tcggactcga aatcgatgtc gatgtcgggc ttttgggcgc
       61 gctccaggct catgaatctt tcaaagagga ggcctcgggc tatggagtcg acgttcgtca
      121 gcttcaggca gtatgcgacg gctgagtcag ccgcagatcc gcgccctgca tagcggattc
      181 cttgcgattg ggcccactgc gcgatatccc acaccacgag gaagtagtct tcgaacccga
      241 gggcggtgat tatgctaagc tcatgactta ggcggttttc aatcacagga gtgatctttc
      301 catactgggc tatggcgcct tcccgtgtga ggcgcacgag gtattcggct gatgagaggc
      361 cttttggtgt ctgatacttc gggaaaaggc gcagcgataa atcgagcccg ggtgagcatt
      421 tgtcagcgat ctcattggtg tttgccagtg cttcgggata tttagcgaaa agctcggaca
      481 tttcatcagc tgatttcaga tagttctccg cgtttatccg gcgctctgga tgtatgtcat
      541 ctagggtggt gagcgtccgt acgcatgtaa gggcgtcatg cacttcaaaa cgaggtttgt
      601 tggcgtaatg gacattgttt gtggcgactg ttcccactcc gatctcttgc gcaagctctg
      661 ccaaggcggc attgagggat aatgctctag gtgtcaggga atgttcaagt tcgatataga
      721 agttctcctt gccgaatatg gaggttaggt tttctgctgt gtctttggcg tcctcaaacc
      781 tgctggatgc gacaagggat gggatcttgc cccggcggtt gcatcctgac agagccatta
      841 cgccttcagt atactttgcc aaggtctcca tgttggttct tggctcgcgg cggggggagg
      901 acatatgtgc ctcagtgagc atgcgacata tgttggcata accctcgggg ttttgggcca
      961 gaagagtcag atgcgagcca tctgacatgg aaacctcaac tccatgtata ggctttatcc
     1021 cgtattttgc agcggctttc gataactcta ctgctgcgct tacattgtta tgatcggtta
     1081 tggctattgc agacataccg cactctgcgg cgcgagccac tagtgtgctg caagtatcgg
     1141 ctccgtccag aaaggagtat tgcgagtgaa cgtgtagatg gacgaagggt tgcacgggta
     1201 ttgcctccat agtcaaagat gtgatggatc ttctcggcgg tctactcccg atcgcaacag
     1261 cgaggaggct aatctagcac tcgtgaaagc acccactggc cgggcgcttc gcggcatagt
     1321 tcgtaacatc cgcctgatgc caaagagaca agatagtaac tcttttcttc ctcgccgtcc
     1381 caccattctc ccgattcccg ccaggagtcg actatgccgg tcacccggtt ccacctgttg
     1441 gaccagtaaa actgggcggg cgcaccattt tgagcctcaa tcacacgaac ttgcgcccat
     1501 atgcgtttac tcataaagcc ccatcactcc cagaatattg ccgtagacca ctacaggttg
     1561 ccagctgtgg gcacctatta agcgtacacg tgcatatggg gtttatagga acgattctgc
     1621 aagcatccga tcgcgccttg atactacgag catgtttgcg gtgattacgc tttctgctcc
     1681 gaatcgttcg cgcacatact ggagggcccg gtccatttgg gccttttcgg gttcagtaga
     1741 tgccgaactg aagatggaaa gctgcacctc ttcgggaacg acaagcccgc cgacgcgcgc
     1801 gttaatggct gtgataggca tcgaaacctg gtcttcgatg atccgctcaa gcccgtgggc
     1861 gatctggtat gcagaatcgg taggagagcg gaaagagcgc cgtctgtatt tgccgctggt
     1921 gtagtcattg cgatggctgc ttgcaaactc caggtagatc tctgcccatg atgcgtttct
     1981 tccggagtca cgcagcttgg aggcgatctt ggatgagcaa actgcgatgc actggactat
     2041 gtcggcccag gtttgcactg gagattcgaa cgacatggag cactctatgg attcttcggg
     2101 atatgcggcc tgaacccgtg agtgatctat cccgcagctg agctgatagg ccatgaggcc
     2161 tgcttcgcct aactgcaacg tgagttcagc cataggaacc tttcgcagtt gagcaagggt
     2221 ttttatgccg agcttttcaa ggtgcgccac aacctgagac gggatagccc atacagagct
     2281 cactgggagc ttatcaagaa agcgctgctg ggcttctggc ggtatgcacc ggcatgttcc
     2341 cgcattgtgg atcgtaggcg ttgcagctct tgctacgaat ttggagcttg caaggcctat
     2401 tgatacgatg atgcctgcct cattttccgc tctgtgcatg atgtcgttgg cgattgttcg
     2461 ggctgcgccg cacccggtga aatctgcgaa agcgccgtca ggcgctatgg gttcaaccag
     2521 cggggtgtac tcggcgcaga tgtcccatat tccgttggca agctcggagc atgaatcctg
     2581 ttcatagctt gcgaacacga tctcggaaca tacctgcctt gcatgccttc gcgtagtgcc
     2641 gggagttact ccagtcgcaa gcgcctgggg tgacacgcct ataaccgtgt tctttttcgt
     2701 gacagcgatg ggcttgccgc caagagaagg ttcgagcctg gcatgtgcgg ttgcgtataa
     2761 aaaagggata tgtatgtaag cgatagagcg catttacgat acgcctccgg gtttatgatc
     2821 ggctatagaa catatgttcg gtagactcat tatatcccgg ggacgattgg aaagtcaagg
     2881 agacggatgg cataacgcaa ttccgggaaa atattgggca gcaccgtcaa tcagtcctga
     2941 tcgagtgggt tgatgatgat ataagcaggc ctgccgaggg ctcctatgag cacgtgataa
     3001 cacatgtaga gctgtgttca aggctacgta agacggtaca gcccataact gactaacagt
     3061 tccacgcgtt ccgaaaccga ttgaagacgt ttaaagagtg tgcggacttt gaagaataga
     3121 gcccaccgaa gtatggcttg ttgtgcaatc ttgcatttcg tacacaggag taatcgcggg
     3181 agtggagaat acagacctag ataataactg atatggcgcg gcttggggct tgccgcctta
     3241 gccaagctgt gtcaagattc tatggagggt gaattggaag caatgaaaaa ccgtgttgtc
     3301 gctttcgcgc ttgccatcat cgccattctc tcgctcacat taggttcgtc tttggccgct
     3361 ccgcccaagc acaaagttgc ctacatcata aatggtgcac tgggtgacct ttcgttctat
     3421 gattccggcc aggaaggtat cgataggatc cgaaaggagc tcaatgctga aacaaggacc
     3481 atcgaatgca actttgatcc tgttgcatat cctcaggcgc tccagtcggc agtcgcttgg
     3541 ggggcggatg tgatcttcgt cgtttcctat ggatttgagg accttctcat gcagtacgcc
     3601 gacacatacc ccgataagaa gtggatcaac ctcgacactg tggttgaaaa caagaagaag
     3661 accattacca gcgttgattt tatcgaggaa gaaggcgcct tcatggccgg cgtcgttgct
     3721 ggactcgtaa cgactgacac atccatcccg ggcgtaaacc gcgacaaagt cgtcggtgcc
     3781 gtctgcggcg atgatgatcc ggtcatacga ggttttttgt acggttacga gcagggcgca
     3841 aagcatatcg acaaggatat tgaagtgaag gcgatcttca ctggcacctg ggatgaccct
     3901 gtcaggggca agcaagctgc caagcaactc tattcgcaga aggttgacat catattccag
     3961 atcgccgcgc tcacgggatc cggagtgctt gaggccgcaa aagaagaagg caaatacgcc
     4021 ataggcgtcg attccaacca gaacggaatc cagcccgggc acgtggtaac ctccatgcta
     4081 aaacacatgg gccagtcaat ttatcgtgtt tacggcacga ttttggatgg caaatacaag
     4141 ccagggatgg ttttggaata cgggctgcgg gaaggcggag tcggtcttgc catcgatgag
     4201 cataccagga gaatactccc tgcaaagagc cttcagaggg tcatagatat tgagaacaag
     4261 gtgaagagcg gcgcgatcaa ggtcgaaaga tatcgcggat gattgcgtgg cacattgaag
     4321 ctgcaaatgc gggcgccggc ttcgtctagc cgggtcgtgc tgtcagcggt tgcgttcaac
     4381 acagtgtatg gcaggaaggg aaggctgatt gccgactctt cctgtccctt gttatttcag
     4441 atgcagtgtt ccacagactt ttcttggagg tgcatcgttt cttgcaggac caattcataa
     4501 agatggactc cataacgaag atctatcccc ccgataccat tgccttagat gacgtggatt
     4561 tggttgtcga agaaggagaa atccattcca taatcgggga gaatggggcc ggcaaatcca
     4621 cgctcatgaa ggtgctctat ggcatgaccc cgccaagcgg cggtgagatc agttttcggg
     4681 gccggcgcac aaggttctct acgcctaggg aagccgtggc cgcaggcata ggcatggttc
     4741 atcaggagtt catgctcatc ccttcctaca cggtgtatga aaacgtgatc ttgggcgcag
     4801 aatccgtagg gaggttcggc acactccgcc atgatgatgc aaagcctaaa gtgcaggagc
     4861 ttgttgacac gtatggcctg aacatagaca tcgacagccg aatcagcgat ctttcagtcg
     4921 gcgcccaaca gaaggtggag atactgaagc tcctctatag ggatgttgat gttctcatca
     4981 tggacgagcc aactgcggcg ctcactccgc aggaagcggg ggagcttttc acaagattga
     5041 agagcctcaa cgaggcgggc aagaccatca ttttcatcag ccacaaactt gacgaagtgc
     5101 ttgaattatc tgacaggatc accgtaatgc gcaaaggtaa acgggtgacg accgtggaca
     5161 accgtgggct ggacaaggcg gagcttgcgc gcgccatggt cggaagggat gtcgtgttct
     5221 ccattaagaa gacgcctgcc tccccggaag aagtagtctt tgaagcgcgg agcctaggat
     5281 acatcgacgc gaacggagca accaggctta aggacgtctc tttcgatgtc aggcgcggcg
     5341 agatagtcgg gattgcaggg gtcgagggga atggtcagta cgagttggtc caactcgtta
     5401 cgggcctcct gccggcccag tcgggaagag tcttcatagg aggtcacgac gtgacagatg
     5461 aagatgttat gcagaggcgc aagctcatgt gctatgtgcc tcaagataga aaagtctcgg
     5521 gaagtgccca gagcatgagc cttgtggata atgccatcat gacccaccac cgcctcaacc
     5581 cagagattga ggatcgctgg aaagccctgc tcagttcggg gcgatgccgt gcctttgctt
     5641 ctcgcttggt ggaggacttc ggggttctcg cgccccaact tgatgcgccc atttcatcac
     5701 tttcaggcgg aaatcagcag aaagtggtgg tcggcaggga gttcatgctc gacacggatt
     5761 tcatcctcct cgaccagcct acgcgcgggc ttgatgttgg ctccatagaa tacgtgcagc
     5821 aggaagtagt gcgcaggcgc gatgcgggag cgggatgtct gttgatttct gctgatctcg
     5881 acgagctttt cagcctgtcc gacaggatac tggtgatggc gagaggcagg atcgttgccg
     5941 agcttcgccc ggaggcgacc acccgcgagg agataggcga gtacatgctc ggggcgaaag
     6001 aagctcagaa ggcctggcgc gccgggaagg aggttggaag gtgaagaagc agttactgcc
     6061 tgcaatcatt ggaatagtcg ttgccctttt ggcaggcctc attatcctgt gggcccaagg
     6121 gtatccgcct ttgcaaagct atgcggcgct cttttcctat agcctgtttt caaggtttgc
     6181 attcttttcg actctggaca aggcaacgcc gctgatactg accggccttt cagctgctgt
     6241 ggggttcgga agcaactgcg tgaacctggg ccaaccgggt cagtttttga tcggcgcaat
     6301 ggcagttacc gcttttggaa tagcggtgga tcttccagcg gcaatcatga ttcccctgtc
     6361 aattttggtt gcgcttgccg ccggcgcatg ttggtctgga attgccgcag gcctgaggcg
     6421 acgtttcaac atggacgagt tcatcactac tttgatgctt aactttatcg ctgactattt
     6481 cacattgtat ctaatatccg gtcctatgct ggatacgaag atgtactctc cgctaacccc
     6541 catgacgaat tccggggcat ggcttccgat atggaatggc tttggctctg catttctcgt
     6601 ggcggttgcg gcttttgcgg ttgtctctgt tctgtggaac cgctcgaaga tcggttatga
     6661 atggcgagcg atggggcaga acccgatctt ctcgagaacg ggcgggtgtc gaaacgatg
//
LOCUS       DAOSAI010000085         6714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7305_length_6714_cov_4.049707, whole genome shotgun sequence.
ACCESSION   DAOSAI010000085 DAOSAI010000000
VERSION     DAOSAI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7305_length_6714_cov_4.049707"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..413
                     /gene="ruvX"
                     /locus_tag="PLM83_04285"
     CDS             3..413
                     /gene="ruvX"
                     /locus_tag="PLM83_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOK70659.1"
                     /translation="MGIDLGESRIGVAVSDELGIAAMPHTVIESRGWEQDIIRIGELA
                     KKVGACEIVVGMPRNMDGSYGPAAQRVEDFCERLERAVKLPVVTWDERLSTVEASRLL
                     IEADLSRRRRRQTVDKTAAAIILQGYLDWLGIED"
     gene            564..881
                     /locus_tag="PLM83_04290"
     CDS             564..881
                     /locus_tag="PLM83_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOK70660.1"
                     /translation="MPDEVSIVTLVDENGEEQDFAIDDIVEVDETRYAILVPAECLDE
                     LEDEDVCEEAFIFRIEVDDDGEEYFTDIEDDEEYERVLEALEAFDDDFLDDDYGEDDD
                     EDM"
     gene            1211..2269
                     /gene="mltG"
                     /locus_tag="PLM83_04295"
     CDS             1211..2269
                     /gene="mltG"
                     /locus_tag="PLM83_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOK70661.1"
                     /translation="MPGTVQTRERARRRLAWPLVFLMALLVTAGAWAIWASRGVSPEE
                     GAEVRVRVVLPDGASASKIAAILKEDGLIRSSVMFDAMARFSGKDSSLKAGEYEFIQG
                     ESVASILDAIASGRVVTVRVTIPEGLTALQIADIFHERGLADREELLALVNNPPHDLA
                     EEFPFVADQSSLEGYLFPDTYEFANGVGALRIVRTMLRRFQHTAQAAFDHHPSRPMSL
                     TDRETLILASIVEREAKHSEERPIIARVFLNRLQKQMKLQSCATVQYALPVPKEHLLN
                     PDLEIDSPYNTYINMGLPPGPICNPGLASIEAVLNPEPGDYLYFVAASDGSHVFTKTY
                     SEHLRAKARIERDSRSSR"
     gene            2303..>2840
                     /gene="udk"
                     /locus_tag="PLM83_04300"
     CDS             2303..>2840
                     /gene="udk"
                     /locus_tag="PLM83_04300"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020754577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOK70662.1"
                     /translation="MSDEVLVVGIAGGSGSGKTTVVRSVSQAFGDAVAVLEHDAYYRD
                     QSHLSFEERLKTNYDHPFAFDTDLYISHIETLIEGKPVEKPVYSFESYTRTPQTERVD
                     PAPVIVLEGILILEDPRLRALMDIKAYVDADPDVRFIRRLMRDIKERGRTVDSVVEQY
                     LDVVRPMHLQFVEPNKRYA"
     assembly_gap    2841..2850
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3203..4771
                     /locus_tag="PLM83_04305"
     CDS             3203..4771
                     /locus_tag="PLM83_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOK70663.1"
                     /translation="MAAKAVEQRSVDAVIRLARGPIFDRNMELLAGGTSIGLGGNLGE
                     DCAALAVAIFPQSISGPGDSLDKLERMIPGVLDGAPRVIRLPGYDRKEIVGMAKAVTG
                     IVPISIPSRWSGLASHVVGYLTGSQVGAEGIELAFERFLGGGGQGRDVVRAMCDSRGK
                     EIPGLGVRLAVAGEEPGWVRLSIDRRVQEAVERAFDRRAPGLAGAVVVMDPWTGQVMA
                     MASRPSYDQNDVSSSLARADAPLLNRAVYPYYPGSIFKVVVAAAALNEGARMPGSVYH
                     DSGAIQAGGHTFRCHAFERGGHGDITFADAMAYSCNTAFVDAALAVGPGPIVDAARAL
                     GIGSYEVAASTCLPNVSTGQTPPRQARMAAAELANFALGQSVVKVTPLEVATMMCAVA
                     NDGILRHPTLVLEAVHSASGRVMKMDWEAARALDSGVASKLRSMLSGVVSYGTAQDAL
                     GGRNAAGKTGTAQTGRVTETGQPIYNAWFAGFAPADHPKVVISVLVEGAESGADLAAP
                     IFGDICDEVLGFFG"
     gene            4878..5579
                     /gene="sigK"
                     /locus_tag="PLM83_04310"
     CDS             4878..5579
                     /gene="sigK"
                     /locus_tag="PLM83_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HOK70664.1"
                     /translation="MSSALVAFADLLTRGLMLLHSYVAGNGAFPPPLTEDEERQMLER
                     KEAGDEEARNILVERNLRLVAYVVKKYDVVGEDTDDLISIGAIGLIKAINTFDRQKKT
                     KLATYAARCIENEILMHLRSGRKNRGELSLYDPIGADKEGNEIILMDVLTNGGDTVAD
                     IVESKVEQERLVEKLAKLSPRERRVLELRFGMGEGRRQTQREIARALGISRSYVSRIE
                     KRALAKLMKEMSTPS"
     gene            5786..6322
                     /locus_tag="PLM83_04315"
     CDS             5786..6322
                     /locus_tag="PLM83_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOK70665.1"
                     /translation="MRFCHGIMGYGERRNHDLLKPDLRVSGVWEIDLDDLLARGISGI
                     ILDIDNTLAPWGSYDIPGPVREWIEEAKARGFHLCILTNNYGARADWFSRELDVGAVR
                     GWVKPWAWGYRRAMRKLGTDADSTVMVGDQLFTDIRGANRLGIYSVLVLPLSDRELPH
                     TRFKRTLERKILGDIDER"
     gene            6331..>6714
                     /locus_tag="PLM83_04320"
     CDS             6331..>6714
                     /locus_tag="PLM83_04320"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOK70666.1"
                     /translation="MDGRYAQVVVDVPARRVDKCYDYIIPDNLMDKIVPGSRVVVPFG
                     PRSVEGFVVARTEKPAVAKVKPITACVKSAPPVPEELLAVARWMADEYMCLLIEALRA
                     LVPSGTKLESSTIVRVCATDEDAEGA"
BASE COUNT         1532 a         1653 c         2044 g         1475 t
ORIGIN      
        1 gtatcgggat cgatctcggt gaatctcgaa taggagtggc agtctctgac gaattaggca
       61 ttgcagccat gcctcatact gtaattgaaa gtcgcggttg ggagcaggat attatccgca
      121 taggagaact cgccaaaaag gtcggggcat gtgaaatagt tgtgggcatg ccgaggaaca
      181 tggatggaag ctacggaccg gcggcgcagc gggttgaaga tttctgcgag cgactggaga
      241 gggcggtgaa gcttcctgtg gtcacgtggg atgagcgttt gagcacagta gaggcatcta
      301 gactgctgat cgaggccgat ctttcgcgga gacgccgacg gcagaccgtt gacaaaactg
      361 ccgccgcgat tatcctccag ggttatctcg actggcttgg cattgaggac taggttggga
      421 gtcagccctg cgccagccgg acagttccgg cggcattgac ctggaggaca ggaaaaacag
      481 gaatcgcgga tagaacgagt tgtccttgtg tgggtagagt actgtcttgg cgaacaaaat
      541 agggctagat tggagtgatc gagatgccag acgaggtgag catcgtcacg ctagttgatg
      601 agaatggtga ggagcaggat ttcgcaatcg atgacattgt agaggtggac gagacgcggt
      661 acgctatact cgtgccggca gagtgcctgg acgagctgga ggacgaggat gtctgcgagg
      721 aggcctttat attccgaatc gaagttgatg acgatggaga agagtacttc acagacattg
      781 aagacgatga ggagtatgag agggtcctcg aagccctgga agccttcgac gacgattttc
      841 ttgatgatga ctacggcgag gatgacgacg aagacatgta gccgcctcta gcgcgggcgc
      901 aattggggca gccgaaagca ataagtatgg tgacgccagg taatcggcct ggcgtcacca
      961 ttttttcagc gtttcgttgc tgttttacgt gggactgtat cccgcatgcg aaatgccgca
     1021 aaaggccgtt tcggagacat ccaatctgcc tcacctttcg gttgtctgaa aaggccccca
     1081 ttgaagtcgg aggaatcagt cgccatattg ttgcggatca tgccgagctt ccttgcacaa
     1141 caacgcggtt gaccctataa ttattgttgg tcccaggggt gcacataata ggggacgggg
     1201 ggccttcaga atgccgggta ctgttcagac cagagaaaga gcccgtaggc ggttggcgtg
     1261 gccgttggtt tttcttatgg ctttactcgt gacagcaggc gcgtgggcga tatgggcgtc
     1321 caggggtgtt tcgcccgaag agggtgctga agttcgagtc agagtcgtct tgccggacgg
     1381 tgcgtcagcg tctaagatcg ccgcaatcct taaagaagac ggactcatcc gttcgagcgt
     1441 catgtttgat gccatggctc ggttttctgg taaggactcg tcgctcaaag ccggggaata
     1501 tgagttcata cagggcgaaa gcgttgccag cattctagat gccatagcta gcggaagagt
     1561 agtcacagtc agagtaacga taccggaagg cctgaccgcg ttgcagatag ccgatatttt
     1621 ccacgagcgc ggacttgctg acagggaaga gctccttgct ctggttaaca atcctccgca
     1681 cgacctagcc gaggaatttc cgtttgtagc tgaccaatca tcgttggaag gatatctttt
     1741 ccctgatact tacgagtttg ccaatggagt gggcgctctc cgcatcgttc gaaccatgtt
     1801 gagaagattc caacatactg cgcaggctgc gtttgaccat catccatcac gtcccatgtc
     1861 cctgacggac agggaaacgt tgattttggc gtccatcgtt gagagggaag cgaagcactc
     1921 ggaagagagg ccgattatag cgcgggtgtt tcttaaccgt ctccagaaac agatgaagtt
     1981 gcagagctgc gccacggtcc agtatgctct tcccgtcccg aaagagcacc tcctcaaccc
     2041 ggatttggag attgattcgc cctacaacac ctacattaat atgggacttc cgcccggccc
     2101 gatctgcaat ccaggcctgg catctattga ggccgtgctc aatcccgagc ccggcgatta
     2161 cctctatttt gttgctgcaa gcgatggcag ccacgtattc accaagacat acagcgagca
     2221 tctccgggcg aaggcaagaa tcgagagaga ctcgcggtcg tcgcgatgac aggggcaaca
     2281 caaggcggtg caagaattcc aaatgtcaga tgaagtacta gtggtgggga tcgcgggagg
     2341 atcaggttca ggtaaaacca cagtggtcag atcagtctcg caagcttttg gggatgccgt
     2401 agcagtcctt gaacacgatg cctactatcg tgaccagagc cacctcagct tcgaagagag
     2461 gttgaagacc aattatgacc atccgttcgc gttcgatacc gatctttaca tatcacacat
     2521 cgaaactctc atagagggca aaccggtaga aaaacccgtg tattcctttg aatcctatac
     2581 ccgcacccca cagactgagc gagtcgatcc tgcgcccgtc atagtgcttg aaggcattct
     2641 catactagaa gaccctcggt tgcgcgccct aatggatatc aaggcttatg tggacgccga
     2701 ccccgatgtg agattcatca gacgattgat gcgcgatatc aaggagcggg gcagaacggt
     2761 ggattcagtg gtggagcaat acctagatgt agttcgtcct atgcacctgc agttcgtgga
     2821 gcccaataag agatatgcgg nnnnnnnnnn atcaagtggc aatagagttg cttatcgcga
     2881 ggctcaagtc ggcaatcagt tcagattgac gggcttttgc ccagattgat cagtctgggc
     2941 aagtctcaaa gaatatctga ccactccgag agctcaggga ccgagcccaa ctccttttgg
     3001 ccagtcatcg agcctcttca ccatcctttc ctctgagccg tgatggatgc ttcctgaagg
     3061 ttttggggga atgataggat ggggggtggg tgcaacgaga cgaaacgctt caaggctcaa
     3121 tttcctaatt ggcctcgtta ttgtgtgctt tggattgctt ctggttcgcc tcgcctggat
     3181 ttcattggta agaggccggc ctttggcggc aaaagcagtg gagcagaggt ctgtagacgc
     3241 cgtgatccga ctcgcaagag ggccgatctt cgacagaaac atggagctac ttgctggtgg
     3301 cacctctatt ggattgggtg gcaatttagg cgaagactgc gctgctctgg cagtcgccat
     3361 attccctcaa tcgattagcg ggccgggaga cagtttggat aagctcgagc gcatgatccc
     3421 gggcgtgctt gatggagccc cgcgagttat ccggcttccc ggatacgatc gcaaggagat
     3481 tgtcggcatg gcgaaggcag ttacaggcat tgtccccatc agtattccca gccggtggtc
     3541 tgggctagcc tcgcatgtag ttggctacct tacggggtca caggttggcg cagagggaat
     3601 agaactcgcc ttcgaacgct tccttggtgg tggcgggcag ggtagggacg tggttcgggc
     3661 gatgtgcgat tccagaggca aggaaatacc tggccttgga gtccgattgg ctgtggcagg
     3721 agaggagcct ggttgggtaa ggctctctat agaccggcgc gttcaggagg cagtagagcg
     3781 agcattcgac aggcgcgctc ctggactggc cggggcagtg gtggttatgg acccatggac
     3841 tggccaggtt atggcaatgg catcccgccc gtcgtatgat cagaacgacg tttcgagctc
     3901 tctggctcgc gccgatgcgc cattgctcaa tagagctgtc tatccctact accctggctc
     3961 aatcttcaag gtcgtggttg cggctgccgc attaaacgaa ggcgcgagga tgccgggatc
     4021 tgtctaccat gattcagggg cgatacaggc aggcggacac acgttcagat gccatgcttt
     4081 cgaacgtgga ggtcacggcg acatcacatt cgcggatgcc atggcatact cctgcaatac
     4141 cgcgttcgtc gatgcagccc ttgccgtggg ccccgggcct atcgtggacg ccgctagagc
     4201 cctgggaata gggagctacg aggttgctgc atcaacatgc cttccaaatg tgtccacagg
     4261 ccagactccc cctcgacagg cgcgcatggc cgctgcagag ctagccaact tcgccctggg
     4321 acaatcggtt gttaaggtga cgcctctgga ggtcgcgacg atgatgtgcg ccgtggctaa
     4381 tgacgggatc ttgcgtcatc cgactttggt gctcgaggca gtgcactccg catcaggcag
     4441 ggtaatgaag atggactggg aggctgcccg cgcgctcgat tccggtgtcg cctccaaact
     4501 tcgctccatg ttatcagggg tggtatccta tgggaccgcg caagatgcgc tggggggacg
     4561 gaatgcggcg ggcaagacag gaaccgccca gacaggcagg gttacagaga caggacagcc
     4621 catctacaat gcttggtttg ccggattcgc gccagccgac catcccaaag ttgtcatttc
     4681 ggtgctggtg gaaggggcgg aatcaggtgc agatcttgca gcccccatat tcggagatat
     4741 ttgtgacgaa gtcctcgggt ttttcggcta gcctagttgt taggaccggg gcacctcgga
     4801 cgtgcaccga atctctcgtt tcctcataag ctaaatctgt atcacaaagc gttgagggac
     4861 ggaggtggcc tgtcttgatc tccagcgccc ttgttgcttt tgcagacctt ctcacaagag
     4921 ggctcatgct tctccattct tacgttgcgg gaaacggcgc tttcccgccc ccgctcacag
     4981 aagatgagga acgccagatg ctggaaagga aagaggccgg ggacgaagag gcccgcaaca
     5041 tactggttga gcgcaacctg agactagtcg cgtacgtagt taagaagtat gacgtggtcg
     5101 gcgaagatac tgacgacctc atatccatcg gtgccatcgg attgatcaaa gcgataaaca
     5161 ccttcgatag gcagaagaag acgaaacttg cgacatacgc agcacggtgc attgaaaacg
     5221 agatacttat gcatttgcgc tcaggacgaa agaaccgggg agaattgtcg ctatatgacc
     5281 caataggcgc cgacaaggaa ggcaatgaga taatcctcat ggacgtgctc accaatgggg
     5341 gcgacaccgt tgccgatatt gtggagtcta aggtggaaca ggagagactt gtggagaagc
     5401 ttgcgaagct gtcgccacgg gaacggagag tgttggaact cagattcggc atgggagaag
     5461 ggcgccgtca gacccagaga gagattgcgc gcgcattggg gatatcgaga tcttatgtgt
     5521 cgaggataga gaaacgtgct ctggccaaac tgatgaaaga gatgtccaca cccagctagc
     5581 atccgagtcg gcgacaccca tacggcctat tccagttctc gattggcacc tgatctgcca
     5641 tggagattcc gcaacgagcc ggctgcccgg ccaccaatta gcacatgcga ctcctctcgc
     5701 tgaggccgca ctcacctgta gcgcattctg aggcgacgat aaacgtctat gggtccgtgc
     5761 gggctgtggg cgtacgcatc gcagaatgag gttttgccat ggtataatgg gctatggcga
     5821 gaggaggaat catgacctgc ttaaaccaga ccttcgagta tccggagtat gggaaatcga
     5881 cctggatgat cttcttgcaa ggggaatatc aggtatcatt ctggatatcg ataatacctt
     5941 ggctccttgg ggcagttacg acattcccgg tccagttcgc gagtggattg aggaggccaa
     6001 ggccaggggc ttccacctgt gcatcctaac caacaactac ggcgcccgcg ccgattggtt
     6061 cagccgcgaa ctcgatgtgg gcgccgttcg gggttgggtt aagccgtggg cgtggggcta
     6121 caggcgtgcc atgcgcaagc ttggaactga cgctgattct acggtgatgg ttggagacca
     6181 gctttttacc gacattcgcg gcgcgaatag gctcgggata tactctgtat tagtcttgcc
     6241 cctcagtgac cgggagcttc cgcacacccg attcaaaaga acccttgaaa gaaagatcct
     6301 tggagacata gatgagaggt gacggccacc ttggacggaa gatatgctca ggtggttgtg
     6361 gacgtgcctg cccgcagggt ggacaagtgt tacgactata taattcctga caacttaatg
     6421 gacaagatcg tgcctgggtc gagggtcgtg gtgcccttcg gacccagatc tgttgagggc
     6481 tttgtcgttg ccagaacgga aaaacctgcc gtggcaaagg tgaagccgat aactgcgtgc
     6541 gtcaagagtg cgccccctgt cccggaagaa ttgctcgccg tggccagatg gatggcggat
     6601 gaatacatgt gtctgttgat cgaggcgctt cgggctttgg tgccgtcagg cacgaagctg
     6661 gaatcatcca caatagtgcg cgtgtgtgcg acagatgagg atgcggaagg ggcg
//
LOCUS       DAOSAI010000086         6675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7365_length_6675_cov_4.131571, whole genome shotgun sequence.
ACCESSION   DAOSAI010000086 DAOSAI010000000
VERSION     DAOSAI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7365_length_6675_cov_4.131571"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PLM83_04325"
     CDS             complement(<1..1104)
                     /locus_tag="PLM83_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOK70667.1"
                     /translation="MPQFQLKSGFAPAGDQPRAIAQLVAGVKAGMRGQVLLGVTGSGK
                     TFTMANVIQELGRPTLVIAHNKTLAAQLASEFREFFPNNAVHYFVSYYDYYQPEAYIP
                     QSDTYIEKDAQINDEIDRMRHAATAALVERRDVIIVASVSCIYGLGMPEEYLNQAVVV
                     NAGEYLDRDRLLRKLVGIQYSRNDIGITRGTFRVRGDVVEIIPASGEEAIRIEFFGDE
                     VDRISDIDLITGEVLGLRESAFIYPATHYVTSKERLDAAIEAIGVELAERLKELKRDG
                     KLLEAQRLEQRTMFDIEMLEEVGYCSGIENYSRHLAGREAGSAPATLLDYFPDDYLMF
                     IDESHVTVPQIRAMYNGDRSRKESLVQYGFRLPS"
     gene            complement(1518..3176)
                     /locus_tag="PLM83_04330"
     CDS             complement(1518..3176)
                     /locus_tag="PLM83_04330"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOK70668.1"
                     /translation="MKRKPYSLWVAVLACLAMTLTGCGKEVKVVHEDGQTIYDYSRKS
                     EKLSSELTKALLESGVIDKSVAANGRQEKIMRLSSKEDTYLRWTITSYEAGASSASYQ
                     SLLAAVSRAARKANARVIQEIADGDSSAGDCAIFILGFSDRSLQGLATYLEIMQVVVP
                     WSRSLGQTVAPTRNEQYLESIQGLDVSETFSIEETASGRDEALLDLEEMPVGAGSVLS
                     KAQENVVPRYISKEPPLSSSERKTLNGSRPRLAIVIDDLGSGVAATDELLSIDVPLTV
                     AVIPQGKYAAKEAKAASERGFAVLLHQPMEPLDPSKRPGKGGILMGMDEKEIRSVISS
                     NLALVPNAIGVNNHMGSRVTEDPESVRIILDEVFAHGLFFFDSRTSSNSVVPRVAREM
                     GLPLLENSRFLDHIDTEEYVIESIRIVARLAMERGSAAAIGHVRPATVRAIQKMLPEL
                     DAAGVELVTLDKLMPTPSRTAEPKPSLPAVPATVKEAAPAAPVQLPVEADKEEAGIEA
                     LPEVEPQAFSEPEPRQELEPEPGECGETNTGQTVDDPGLDLSEP"
     gene            complement(3173..4465)
                     /locus_tag="PLM83_04335"
     CDS             complement(3173..4465)
                     /locus_tag="PLM83_04335"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOK70669.1"
                     /translation="MQEFPFGNVFRIILESQIALFRYPSSMIWFWIVMSIVYFQYRRM
                     SALETQIFGRPRRSAIVRTMCSLGEGMVGGILGSFLIILFGASAESLGLGAIWLIALA
                     LASIDIRFICFSYAGGIVSLLNLAFGWPDVDVTSIMALVGVLHLVESALILLSGHRDA
                     APVIVSGPDNKPAGGFYMSKYWPVPIAILWITYSLATTGTPEGGIAMPDWWPLLKSAA
                     PPSPDMRADYWVLAITVAMGYGDATYSRLPRQKTKATAGMSALFSLALLGLTLLSERY
                     PWVRWVAALFSPIGHEFVIGMSQKAELEGEPLFTTPAQGIRVLDVGPGSPCDQAGIKP
                     GDVIEAVNCQPVNSFADFAEALRSVGYATILDIRSGLPGSKGENRTVQIRGVPDGLGL
                     LLVPTEGDYPKMDSGRAGLIAIIVDRIKRRSSRRRRRR"
     gene            complement(4481..5797)
                     /locus_tag="PLM83_04340"
     CDS             complement(4481..5797)
                     /locus_tag="PLM83_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK70670.1"
                     /translation="MSSYKKAQRSAWLVLTVAAAILVCTVLAPSAFGVPSPQDEEAAP
                     KFKDIHTAIEAMYLVKAQFYKPVSVAELMKAYFAKGTVPGMLEVLNDPYTRYMTPQDF
                     QALKDDSAGIFGGIGILVGIRDDKITIISPITNTPGMRAGLMPGDHIVEIDGVSTEGM
                     VLDRAVSMMRGEPGTVVRLLIERRYPAEKFEVEIVRDIIDAPATRGYIFDPDAGIGYI
                     ELRTFSQKAGQDIEEEVERLMDEGMRGLILDLRYNGGGLVTQAVEVASKFMPPGPVMH
                     VRGRDGQKKSLYTVGRRVSQIPLVVLVNEYTASASEIVSGALQDTGVATIVGVPTFGK
                     GLVQTLFPLSDGSGLAVTTQIYLTAGGREITPETPIMPDVKVEALTPEELEKAMNDQA
                     RNAERTGPVTAEDLMRIDPDLDPQFRRALEILRYQIEKDEPVKSAA"
     gene            complement(5878..>6675)
                     /locus_tag="PLM83_04345"
     CDS             complement(5878..>6675)
                     /locus_tag="PLM83_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOK70671.1"
                     /translation="IYKLGPGTVLELLFGASSFSDFVSRYDYLGAIAKQDADIYGEIR
                     AEKESLEEQKEGICARKAKLEETRREIALLSSALEDERRSIEPVLAQRSQYASRVSAE
                     RERWERELAEEERASRELERTIREMQARTGGTISWTGKFVWPVTARISSPFGWRIHPI
                     FKNKRFHSGIDLAASSGTPVKAAADGKVLLAGWVNGYGNTVILDHGNGLSTLYGHASV
                     ITTKAGNVVLQGDIICKVGSTGYSTGPHLHFEVRQDGTPQDPMRWLP"
BASE COUNT         1522 a         2005 c         1584 g         1564 t
ORIGIN      
        1 agatggaagc ctgaatccgt actggaccaa gctttccttg cgcgaacgat cgccattgta
       61 catcgcccgt atctggggca cagtgacgtg cgattcatcg atgaacatga ggtagtcatc
      121 gggaaaataa tccagcagcg tcgcaggagc actgcccgct tccctgccgg caagatggcg
      181 cgagtagttt tctatcccgg agcaatagcc cacttcctcc agcatttcaa tgtcgaacat
      241 ggtccgctgc tccaacctct gggcttccag gagttttcca tcacgcttga gctccttgag
      301 cctctccgcg agttcaaccc cgatggcctc aatcgccgca tcaagcctct ccttgcttgt
      361 aacgtagtga gtggcagggt agatgaaagc cgattccctc agtccaagca cctcgccggt
      421 aatgaggtca atgtcggaaa tgcgatcaac ctcgtcgccg aagaattcta tgcgtatcgc
      481 ttcttccccg cttgcaggta tgatctccac aacatcgccc ctgacgcgga aagtgccacg
      541 ggtaatgcca atatcattgc ggctgtactg gatccccact aacttgcgga gcagcctatc
      601 tcggtccaag tattcgcctg catttaccac gactgcctga ttgaggtatt cctcgggcat
      661 gcccaatcca tagatgcacg aaacactcgc tacgattatt acgtcacgcc tctcaaccag
      721 agcggcagta gcagcatggc gcattcgatc aatctcgtca tttatctgag catccttctc
      781 gatgtatgtg tcagactgag gtatataggc ctcaggctgg taataatcgt agtagctcac
      841 aaagtaatga acagcgttgt tggggaaaaa ctccctgaac tccgacgcca attgtgcagc
      901 aagagtcttg ttgtgagcaa tgaccaacgt aggtcggccc agttcctgga tgacattggc
      961 catcgtgaac gtcttgccac ttcccgtgac tccaagcagg acctgcccgc gcatgcccgc
     1021 cttcactccc gcaacgagct gcgcgatagc cctcggctga tcgcctgcag gggcaaaacc
     1081 cgatttcagc tggaattggg gcattgcaga ccactccttt cagcggatga cggctcaatt
     1141 atatcacgcc ccgaacatac attctatccg cgataacagt ttaatgcgcc aaacaatcat
     1201 ccggaagcat ctaaagctca cattgccagg attcccccac ttcccatgct cctcaataag
     1261 cccttcagct cgtcaccacg caattcttcc ctgttcccct gcaaaagccg agattatggc
     1321 tccaccgcct tgccatcaat acggctgcag cacgcgaacc ttgagcacaa tcacggcacg
     1381 ttctcccata caccctaaac ggcagaaacg cgccccaaca aaactgtagc ggcccctcgg
     1441 ggccgcattc cttctcagtc tatttatttg tgtgacttta gatctggacg atccggacac
     1501 aaccagccga aggccggtta aggctctgat aggtcaagcc cgggatcatc tacagtctga
     1561 cccgtattcg tctcgccaca ctccccaggc tcgggttcta gctcctgcct gggctcaggc
     1621 tcagaaaagg cttgaggttc gacttctgga agagcctcaa taccagcttc ctccttgtcg
     1681 gcttcaacag gcaactggac aggcgctgcg ggagccgctt ccttgacggt ggctggcact
     1741 gcgggcagac tcggtttcgg ttctgccgtt cggctcggcg tcggcatcag cttatcgaga
     1801 gtcactagtt caaccccggc agcatccaac tcgggaagca tcttctgtat tgcacgtact
     1861 gtggcaggac gcacatggcc tattgcggca gcggaacctc gctccattgc caacctcgct
     1921 actatcctta tggattcgat aacatactcc tcggtatcta tgtgatctaa aaacctagag
     1981 ttttcaagga gcggaagccc catctcacga gccacccgcg gaaccaccga gttcgagctc
     2041 gtccggctgt cgaaaaagaa caagccatga gcaaaaacct catcgagtat aatcctgaca
     2101 ctttccgggt cttcagtgac acgcgaaccc atgtggttat tgactccaat agcattgggc
     2161 accaaagcta aattggacga tatgacgctc cgtatctcct tctcatccat acccatcagt
     2221 atcccgccct tgcccgggcg ctttgacggg tccaatggct ccataggctg gtgtagtagg
     2281 actgcaaagc ctcgttcgga agcggccttg gcctccttcg cagcatattt tccctgtgga
     2341 atgacggcca cagtaagcgg cacatctata ctcaaaagct catctgtagc agcaactccc
     2401 gatccaaggt catctatgac tatcgcaagc cgaggcctgc tcccgttaag agtcttcctc
     2461 tcggaggatg acagtggcgg ttccttcgat atgtatctgg ggacaacgtt ttcttgcgcc
     2521 ttggagagaa ctgaacccgc tccaactggc atttcctcca gatcaagcaa ggcttcgtcg
     2581 cgacctgacg cggtctcttc aatactgaag gtttccgaaa cgtccaggcc ttgaatggat
     2641 tccaagtatt gctcgttccg tgtcggcgcc acagtctgcc ccaggctccg gctccacgga
     2701 accacaactt gcattatctc gagatacgtg gcaaggccct gcaggcttcg atcgctgaag
     2761 cccaatatga aaatggcgca atcgcctgca gaggaatctc catcggcaat ctcctgaata
     2821 actctggcat tggccttacg cgccgcacgg cttacagctg ccaacaaaga ctgatagcta
     2881 gcagatgaag cgcccgcttc gtacgaggtg attgtccagc gaagataggt atcttctttg
     2941 gacgaaagcc gcataatctt ttcctgcctg ccatttgcgg ccacactttt gtcgatcact
     3001 ccagattcca gaagcgcttt ggttagctca gatgaaagct tctccgattt gcgtgaatag
     3061 tcatatatcg tctggccatc ttcatgaacc acctttacct ctttgccaca tcctgtcagc
     3121 gtcatggcga ggcatgccaa gaccgccacc cacaagctgt atggcttcct tttcatctcc
     3181 tgcgcctccg tcgactgctg cgtcgtttta tgcgatccac gatgatcgcg atcagtcctg
     3241 cacggccaga gtccatcttc gggtagtctc cttccgtagg cacgaggagc agacctagcc
     3301 catccggaac tcctctgatc tgaacagtcc tgttttctcc tttactgccg ggcaagccag
     3361 atcgaatatc aaggattgtg gcatacccca cactgcgtag cgcctcagca aagtcggcaa
     3421 atgagttgac cggctggcaa ttcacagctt cgatcacatc cccgggcttg atgcccgcct
     3481 ggtcgcaggg agaccccggc cccacatcga gaactctgat gccttgcgca ggcgttgtga
     3541 acaatggctc tccttcgagc tcggcttttt gcgacatacc aatgacgaac tcgtgcccaa
     3601 taggcgagaa cagtgctgct acccatctga cccacggata cctttcagat aaaagagtga
     3661 ggccaagaag cgccaagctg aatagggccg acatgccagc agtcgctttc gtcttctgcc
     3721 ttggcaaacg gctgtatgtc gcatccccat atcccatggc aaccgtaatg gccaagaccc
     3781 agtagtcagc gcgcatgtca ggagatgggg gtgcagcgga cttaagaagc ggccaccaat
     3841 ctggcatggc gattccgcct tccggggttc ctgttgtagc gagactgtaa gttatccata
     3901 aaatcgcaat gggaacaggc caatacttgc tcatgtagaa accgcctgca ggcttgttat
     3961 ctgggcctga tactatgaca ggagcagcgt cgcgatgccc gctgagcaat atgagcgcgg
     4021 attccacaag atggagcacc ccgactaaag ccatgattga tgtgacatcc acatccggcc
     4081 acccaaaggc caggtttaga agggacacta tgccgccggc atacgagaaa cagatgaacc
     4141 tgatatctat cgatgccagc gccaatgcta ttaaccaaat cgccccaagg cccaaactct
     4201 ccgctgaagc gccaaacagg attatcagaa aactgccgag tattccccca accatgccct
     4261 ctcctaggga gcacatggtt cgcactatcg cactccgcct cggtctgcca aatatctgcg
     4321 tttccaaggc cgacattcgc cgatactgga agtatactat gctcattaca atccaaaacc
     4381 atatcatact ggagggatag cgaaacaacg ctatctggct ctccagtatg attctgaaca
     4441 cgttgccaaa tgggaattcc tgcaactgca acactccgct ctacgcggcg ctcttgacgg
     4501 gctcatcctt ctcaatctgg tatcttagga tctcgagagc acgtcggaac tgtggatcca
     4561 aatcaggatc tatccgcatc aaatcttctg cggtaacagg gcccgtgcgc tcggcattcc
     4621 gggcctgatc attcatggct ttctcaagct cttcgggcgt caacgcctcc actttcacat
     4681 ccggcattat tggggtttca ggcgtgatct cccgtcctcc cgccgtaagg tatatctgcg
     4741 tagttaccgc aagccccgaa ccatcagaaa gagggaatag agtctgaacg agccctttcc
     4801 caaacgtcgg cactcccact atggtggcta cgcccgtatc ctgcaatgct ccggaaacga
     4861 tctctgaggc agatgccgtg tactcattga ccaaaacgac caacgggatc tgggaaacac
     4921 gtctgccaac ggtgtaaagg cttttcttct gaccatcgcg tcccctaaca tgcatcaccg
     4981 gtccgggcgg catgaacttg cttgcaacct ctacagcttg tgttacaagg cctccgccgt
     5041 tatatcgcag gtcaaggatg aggccgcgca tgccctcatc catgagcctt tcaacttcct
     5101 cttcgatatc ctgtcctgcc ttctgcgaga aggttcgaag ctcgatataa cctatgcccg
     5161 catcggggtc aaagatatag ccacgggtag caggcgcgtc tatgatgtcg cgcactattt
     5221 ccacttcgaa cttttcggcc ggatatctgc gttcaatgag gaggcgaaca acagtcccag
     5281 gctcgccgcg catcatcgat acagcgcggt ccagaaccat gccttctgta gaaactccat
     5341 caatttccac gatgtgatcc ccaggcatca gtcctgcacg cattcccggc gtattcgtga
     5401 taggactgat tatggtgatc ttatcatccc ttatgccaac aaggatcccg attccgccga
     5461 atatccctgc agaatcgtct ttgagggcct ggaagtcttg gggtgtcata tacctggtat
     5521 aggggtcatt gagcacttcc agcattccgg ggacggttcc ttttgcgaag tatgccttca
     5581 tcagctcagc tactgaaact ggcttataga actgagcctt gacaagatac atcgcttcaa
     5641 tggcggtgtg aatatctttg aatttaggcg cagcctcttc atcctgtggc gaaggcacgc
     5701 caaatgctga tggagccaac accgtgcaca caagtattgc tgctgctacg gtaagtacaa
     5761 gccaagcaga tctctgcgct ttcttatatg aagacatgat tcggcaccca ctttcacaag
     5821 tccatacatc ctatgctaaa ggctctgaga atcgaaccgc caagagggcg tcgatcttca
     5881 gggcagccaa cgcatgggat cttgaggggt cccgtcttgc ctgacttcga aatgtagatg
     5941 aggccccgtt gaatagcctg tcgatcccac tttgcatata atatcaccct gcagcaccac
     6001 attgccagcc tttgtcgtga taacgctggc atggccatat aacgtagaaa ggccgtttcc
     6061 gtggtcaagg ataacggtgt tgccgtatcc atttacccat cccgccaaaa ggaccttgcc
     6121 gtcggcggcc gctttgacag gcgtgccgct gctagcagcc aagtcaatgc ctgagtggaa
     6181 ccgtttgttt ttgaatatcg gatggattct ccagccgaac ggtgacgaaa tcctcgccgt
     6241 caccggccat acgaacttgc ccgtccagct gattgtaccg cccgtcctag cctgcatctc
     6301 cctgatggtc cgttccagct ccctcgatgc acgctcttcc tcagccagct cccgttccca
     6361 tctctcacgt tctgccgata ccctgctggc gtattgcgat cgttgagcca atacgggctc
     6421 gatggacctt cgctcgtcct ccaaggcgga cgacaggagg gctatctctc tcctggtctc
     6481 ctcgagtttg gcctttcggg cacagatgcc ttccttctgc tcctcaagag attccttttc
     6541 ggccctgatc tcgccatata tgtccgcgtc ctgcttcgct attgccccga gataatcgta
     6601 tctgctcaca aaatcagaga agctcgatgc gccaaaaagc aactccagca ctgttcccgg
     6661 accaagcttg taaat
//
LOCUS       DAOSAI010000087         6675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7366_length_6675_cov_3.752115, whole genome shotgun sequence.
ACCESSION   DAOSAI010000087 DAOSAI010000000
VERSION     DAOSAI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7366_length_6675_cov_3.752115"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2867)
                     /locus_tag="PLM83_04350"
     CDS             complement(<1..2867)
                     /locus_tag="PLM83_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOK70672.1"
                     /translation="MYFLLEHVARLAQELKEHVVSAKAPIEGWSIKEGDFSRPDDVDQ
                     ASAEWSKFLPDMRWGGRDSHAWFRADAAISESFAGKPVALNVHTGDKGWDATNPQFLL
                     YVDGKIVQGLDVNHCEAILCSSGEPGRIYRIDLDAYSGMRESRPSLYAELIAIDEKVR
                     KLYYDIVVPLGVVQNLPEDDKTRIDMLLVLNEALNRVDFRSPRSASFHCSIDRAQAYL
                     EEELYGKMCGHGDVLAACVGHTHIDVAWLWRIAQTREKAARSFSTVLKLMDEYPDYVF
                     MSSQPQLYKFVKEDYPALYERIGERVREGRWEPEGAMWVEADCNVVSGESLVRQIMFG
                     KRFFAEEFGVDNKILWLPDVFGYSAALPQIMKKSGIDYFMTTKISWNQYNKLPYDTFM
                     WRGIDGSEVLTHFITTRDYETSPTSWFATYNGDLKPSQVMGAWQRYQQKALNNDVLIS
                     YGHGDGGGGTTREMIENGLRMARGIPGCPRVRMATVRSFFDRLEKTVSGNRRLPTWVG
                     ELYFELHRGTYTSMGRNKRANRKSELLYGDVETLSTFAMLMGADYPQDEINRNWERIL
                     TNQFHDILPGSSIKEVYDDSQEDYDCIISTGHELARNALDAIVSSIGTDDVSVVVFNT
                     LGYDRDEIAEFALPENVEHPCLVDSDGGIIPCQVQAVNGKRIGLFIAHGVPSKGYKTY
                     RLLDRPTQVPSGLAVSSFKLENAYFELQLDEKGTFISIYDKVNQRQVLKKDERGNRLL
                     AFQDMPANWQNWDIDISYQEKVWEVDRVDKMEIVEEGPVRAGIRIHKTFFDSKIVQDV
                     LIYSDIPRIDFKTYIDWKQSQILMKAAFPVDVNAEKAAYEIQFGNVERPTHWNTSWDV
                     ARFEVCGHRWADLSEGGYGVSLMNDCKYGYDIKDGEIRLTLLKSGIEPNPVADQEEHW
                     FTYSIYPHAGDWKTASTTRAAYSLNVPLRAEVVGPQPDGSLP"
     gene            complement(3046..4335)
                     /locus_tag="PLM83_04355"
     CDS             complement(3046..4335)
                     /locus_tag="PLM83_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK70673.1"
                     /translation="MTRIIACAVLVVGLLAGLPGPGAAASYDLKGKTVTFVGWWDYLD
                     KFAPGKEREGRIQEAEKKFNCKIVAKPLNWDDYTSLLMTRLLSGDSEYDVWLMNHNFF
                     WQLAARDAFCPLDKVLPQSYYKSLPKARQGAVKVMSHGGNTYGFGSYGNILGCLRFMA
                     YNKTMFEREGFPDAQKLYEQGKWDWDHCLDIANKATKDLDGDGNPDQWGLSSLWHWGV
                     ALSNDGKLTRADSKGRPIFTGDEINVTEGMAMYLDLYEKYKVCLDDDWGGDQFVAGKV
                     AMQPLEFWRFEFIGPKMKDRWGIVPVPKGPRAADYRYPNLQLESLVLPANSKDPKAFV
                     ALADFLFTAEDHDNDISGSIKAGCPDRVSAEIVKRGVENWKGEMYDLNLGAVAGQVWS
                     DALMAWLNGEKSAAVAMKEVKPAIQTAINDFFAPKKK"
     gene            complement(4503..5474)
                     /locus_tag="PLM83_04360"
     CDS             complement(4503..5474)
                     /locus_tag="PLM83_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK70674.1"
                     /translation="MEATKEAVRGVAPPGQREVRQQHAKRPPFWRKAGRIVWRILRAG
                     LIIGICYVILMPLMTKISSSFMEVRDMFDVTVRWIPRHFTLENYKVVWEAMKYPTAFW
                     NSLKLSLIVAVLQTFSSALVGYGFARFKFKGSNLLFALVLVTLLVPSQTILVPLYLNF
                     RYFNLFGLIPGQGLNLLNSYWPFILIAMTGAGMRGGLFIYVMRQYFRNVPKELEDAAY
                     VDGASPLRTFFTVMLPGAVPSMVTIFLLSFVFQWNDSTLTSVFMGNEVLLPWTLRTLA
                     DTLGARAEYASLVNNTGMLLFMAPLLILYFVMQKHFVEGVERTGIVG"
     gene            complement(5531..6436)
                     /locus_tag="PLM83_04365"
     CDS             complement(5531..6436)
                     /locus_tag="PLM83_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK70675.1"
                     /translation="MKHSTRRLTLERRRAVSGYLFTLPFTIGFLVLFLVPFIQSIAFS
                     LSELRITSTGYQLDYLGLKNFHDAILVQPDFNKKLIEELGKMLRQVPLVIVFSFFAAV
                     MLNEEFPGRSVARTIFFLPVIMSSGVVFFMEKTDYILQTVAAGTQAAVAEGLVGGSAI
                     RAFLWQLKLPEWFMSYIVSAVDNIPVVIRSSGIQILIFLAGLQSIPGSLYEAADVEGC
                     TRWERFWLITFPMLSPLVMTNLVYTIIDFLTGRQSEFLSWIQDFTFRASYGMGTAMAL
                     LYFLAVGIIVAIVVSIVSKWVFYQE"
BASE COUNT         1628 a         1895 c         1537 g         1615 t
ORIGIN      
        1 ggaaggcttc catccggctg tggccccacc acctcggcgc gcaacggcac gttgagtgag
       61 taggcagccc tggtcgtaga tgccgtcttc cagtcgcctg catgcggata gatcgaatac
      121 gtaaaccaat gctcttcctg atcggctacg ggattgggct cgattcccga tttcagcaag
      181 gtcagacgga tctctccatc ctttatgtca taaccatatt tgcagtcatt catgaggctt
      241 acaccgtagc caccctcaga aaggtctgcc cacctgtgcc cgcaaacctc gaaccgtgca
      301 acatcccatg aagtgttcca atgcgtcggc ctttccacat tgccgaactg aatctcgtaa
      361 gccgccttct cagcatttac gtccaccgga aaggctgcct tcatgaggat ctgggattgt
      421 ttccagtcga tgtaagtctt gaagtctatc cgcgggatgt cggagtatat aagaacatct
      481 tgaacgatct tcgaatcaaa aaatgtcttg tggatcctga tccccgcacg tacgggacct
      541 tcctccacaa tctccatctt gtcaactcgg tcaacttccc acaccttctc ctggtatgag
      601 atgtcgatat cccaattctg ccagttagca ggcatgtcct gaaatgccag gagcctgttg
      661 cctcgctcgt cctttttcag aacctgcctc tgatttacct tgtcatagat ggatatgaag
      721 gttcctttct cgtccagctg aagctcgaaa taggcgttct ccagtttgaa actgctcact
      781 gctaaaccac taggaacctg tgttggcctg tccaagagtc tgtatgtctt gtatcctttg
      841 gagggcactc catgtgcgat gaaaaggccg attctctttc cgttcacagc ctgcacctga
      901 caagggataa tccccccgtc gctatcaact aggcagggat gctctacatt ctcaggaaga
      961 gcgaactctg caatttcgtc acgatcatat cccaatgtgt tgaaaaccac gactgatacg
     1021 tcatcagtgc ctatacttga aacaattgca tccagtgcat tccgggccag ctcatggcct
     1081 gttgagataa tgcaatcgta atcctcctgc gaatcatcat acacttcctt aatggacgat
     1141 cctggcaata tgtcgtggaa ctggtttgta aggattctct cccagtttcg attgatctca
     1201 tcttgcggat aatctgcacc catcaacata gcaaaggtcg acagcgtctc tacgtctcca
     1261 tacagcagtt cgctcttgcg atttgctcgc ttgtttctcc ccattgaggt atatgtccct
     1321 ctatgcagtt cgaagtagag ttcgccaacc cacgtaggca gccgacggtt tcccgataca
     1381 gtcttctcga gtcggtcaaa gaaactgcgt acagtcgcca ttctcaccct tgggcagcct
     1441 ggtattcccc gcgccatcct aagtccgttc tcaatcatct ctcgagtggt gcccccaccg
     1501 ccatcaccgt gaccgtaaga gatcagaaca tcattgttca aagccttctg ctgatagcgc
     1561 tgccatgccc ccatgacttg agacggtttc agatcgccat tgtatgtggc aaaccaagaa
     1621 gtgggcgacg tctcgtaatc ccttgtcgtg atgaaatgcg tcaacacttc cgatccgtcg
     1681 attcccctcc acatgaacgt gtcgtaaggg agcttgttgt actggttcca gctgatcttc
     1741 gtggtcatga aataatcgat gccggatttc ttcattatct gtggcagggc tgcactgtaa
     1801 ccgaatacat caggaagcca taggatcttg ttgtcgacac cgaactcctc agcaaagaac
     1861 cgtttgccga acattatctg tcgcaccaaa gactcgccag acacgacatt gcagtcggcc
     1921 tcaacccaca tagcgccttc cggctcccac ctgccctcac ggactcgttc cccgattctc
     1981 tcatacagag ccgggtagtc ctctttcacg aacttgtaga gctgaggctg gctagacatg
     2041 aaaacgtaat cgggatactc gtccatcagc ttgagcactg ttgaaaaact cctggccgcc
     2101 ttctcgcgcg tctgcgcgat cctccatagc caggccacat ctatgtgcgt atgtcctacg
     2161 cacgcggcta gcacgtcccc gtgcccacac atcttcccat acagttcttc ctcgaggtag
     2221 gcttgggccc tatcaataga acaatggaac gatgcggagc gagggcttcg aaaatccacg
     2281 cggttgagtg cctcattgag cacgagcaac atgtcgatcc gggtcttgtc atcttcggga
     2341 agattctgca ctacgccgag gggcacgacg atgtcgtagt aaagcttacg cactttctca
     2401 tcaatggcga tcagttcagc atagagcgac ggccgcgatt cacgcattcc gctgtatgca
     2461 tcgaggtcaa tgcggtagat acgccccggt tcaccgctgg agcagagaat tgcctcacag
     2521 tgattgacat ccagcccctg aacgatcttt ccgtcaacat acagaaggaa ctgggggttg
     2581 gttgcgtccc atcccttatc tcccgtatga acgttgaggg caacaggctt gccggcaaaa
     2641 gactctgaaa tggcggcgtc agcccgaaac catgcatgac tatctcttcc accccaccgc
     2701 atgtcgggta ggaacttgct ccattcagct gatgcttggt ccacatcgtc aggccttgaa
     2761 aagtcgcctt ccttgatact ccatccttct attggagctt tcgctgaaac aacatgttct
     2821 ttcagctcct gagcaagtct ggctacatgt tcaagaagga aatacattgc tgtgccccct
     2881 aacatcgcat gcctgatgat tcactccaaa ggcgcccacc cgaccgtacc tgcagctgat
     2941 tctgccgaag ggagctagcc cctcttggtg cgtcgcggtt gatcctttga gattgagttc
     3001 tgctcggacc cgaatccgag cagaactctt cgatatacac aaagactact tcttcttagg
     3061 agcaaagaag tcatttatcg ccgtctggat cgcaggcttg acttccttca ttgccaccgc
     3121 tgcgctcttc tctccattga gccacgccat aagtgcgtcc gaccagactt ggccagcaac
     3181 ggcaccaagg tttagatcat acatttcgcc cttccagttc tcgactcccc gcttgactat
     3241 ttcagcggat accctgtcgg ggcagccagc cttgatgctg ccagaaatgt cgttgtcgtg
     3301 gtcctctgcg gtgaacagga agtcggccaa agccacaaac gccttcggat ccttggaatt
     3361 ggcgggaaga accagcgatt ccagctgaag gttggggtat cgataatcag cagcgcgcgg
     3421 ccccttcgga acgggcacta tgccccagcg gtctttcatc ttcgggccta tgaactcgaa
     3481 tctccagaac tccaatggct gcattgccac tttgcccgct acgaactggt cgccgcccca
     3541 atcatcgtcg aggcatactt tgtatttctc atagaggtca agatacatag ccattccttc
     3601 agtgacgttt atctcgtcgc ctgtgaagat tggacgtccc ttgctgtcgg cccgggtcag
     3661 cttgccatcg ttcgacagag ccacacccca atgccacagg gacgagagac cccactggtc
     3721 cgggtttcca tcgccgtcaa ggtctttggt ggccttgttg gcaatgtcaa ggcagtgatc
     3781 ccaatcccat ttaccctgct cataaagctt ctgggcatcc gggaaacctt cacgctcaaa
     3841 catggtcttg ttgtaagcca tgaatctgag gcatccaagg atattgccat agctgccaaa
     3901 cccgtaagtg tttccaccgt gcgacatgac tttaaccgcg ccctgtcgcg ctttgggcag
     3961 gctcttgtag tatgactgag gaagaacctt gtcaagggga cagaaggcat ctctggccgc
     4021 caattgccag aagaagttgt gattcatgag ccatacatca tactccgaat cacctgagag
     4081 aagccttgtc atgagcaaag aggtgtagtc gtcccagttc aggggcttag ctacgatctt
     4141 gcagttgaac ttcttctcag cctcttggat tctgccttct ctttctttcc cgggcgcaaa
     4201 cttgtcaaga tagtcccacc agccgacgaa agtcaccgtc ttgcccttta gatcgtaaga
     4261 cgcagcggcg ccagggcctg gcagcccagc caggaggcca actaccaata ccgcgcacgc
     4321 gataatcctt gtcatccgct tcactctttt tacctcccta aatctgctca tgcgactatg
     4381 ctttgaactc gccaattcaa tcgacacggc taaatctagc tgatgctgtt gtcttcaatg
     4441 acactccggc caatcatcac ccccagtttt gtggaccacc ctcagtccac agacctctca
     4501 gtttagccga ctatgcctgt gcgctcgacg ccttccacaa aatgcttttg catgacgaag
     4561 tagaggatca aaagaggcgc cataaacaga agcatacccg tattgttcac aagcgaggca
     4621 tattcagccc gggcccccaa agtgtcagcc agtgtccgaa gagtccacgg cagcaacacc
     4681 tcattgccca taaaaactga tgtaagtgtg ctgtcattcc actggaacac gaatgagagc
     4741 aggaaaatcg tcaccatgct tggcacggcc ccaggcaaca taacagtgaa aaacgttctg
     4801 agcggactcg ccccatcaac gtaagccgca tcctccagct ctttaggcac attcctgaaa
     4861 tactgcctca tcacgtatat gaaaaggccc ccacgcatgc cggctccagt cattgctatc
     4921 aatatgaacg gccaatagct gttgagaagg ttgagaccct ggcctggaat gaggccaaag
     4981 agattgaaat agcggaaatt gagatacaac gggacgagta tcgtctgcga aggcaccaga
     5041 agcgttacca agacaagggc aaatagaaga ttggaaccct taaacttgaa ccgggcaaat
     5101 ccatagccaa caagcgcgct tgagaatgtc tgcaggaccg ccactattaa ggagagcttg
     5161 agcgaattcc agaaggccgt cgggtatttc attgcttccc atacaacctt gtagttttca
     5221 agagtgaagt gcctcggaat ccatctgact gtgacatcga acatgtcacg cacttccatg
     5281 aaggatgacg agatcttcgt catcagcggc atcaatatga cgtagcaaat gccgattatt
     5341 aaccctgccc gaaggatcct ccacactatc cggcctgcct tgcgccaaaa aggcggtctc
     5401 ttcgcgtgct gctgccgaac ttccctttgt ccgggcggcg ctactcctct cactgcctcc
     5461 ttggttgctt ccatttgcac aaccctccac accaagaatg tgtgctgaca ctatcgcctt
     5521 ctgcagacca tcactcctga taaaacaccc atttcgagac gatggaaacc acaattgcaa
     5581 ctatgattcc aacggcgaga aaatagagca gagccatggc tgtgcccata ccgtagctcg
     5641 ccctaaacgt gaaatcctga atccagctga ggaattcgct ctggcggccc gtaaggaaat
     5701 ctattatcgt gtagaccagg ttagtcataa caagtggact caacattggg aaagttatga
     5761 gccaaaaacg ctcccaacgc gtgcatcctt ccacgtcggc cgcttcatac agagatccgg
     5821 gtatggattg caggcccgcg agaaatatga ggatctggat ccccgatgac ctgataacta
     5881 ctgggatgtt gtcgactgca gaaacaatat aggacatgaa ccattccgga agcttgagct
     5941 gccacagaaa agccctgata gcgctaccgc ctaccaagcc ctctgcgaca gcggcctgcg
     6001 tccccgcagc aactgtctgc agtatgtaat ccgttttctc catgaaaaaa actactccgg
     6061 aactcataat cacggggaga aagaatatag ttcgagcaac agaacgccca gggaattcct
     6121 cgttcagcat tacagctgca aagaagctga atactatcac caatggcact tgacgcagca
     6181 tcttgcctag ttcttcaatg agtttcttgt tgaaatccgg ttgcaccaag atagcgtcat
     6241 ggaagttctt taggccaaga tagtcgagtt gatagcctgt cgaagtgatt cgcaattcgc
     6301 tcaaactaaa ggcaatggac tgaataaagg ggacaagaaa gagaacgaga aatccgatgg
     6361 tgaacggaag tgtaaacaaa tagcctgata cagcccgtct gcgttctaag gtcagcctgc
     6421 gcgtggaatg tttcacgtca cgcccccctc aacttgcagc atctgaaggt cagacagtta
     6481 atttcagggt gaaatctaat gtgattatag ccccttctct tttacatgtc aagaaaagtt
     6541 cgtcatttct gggcattcta caccggacag tgtccgacaa taacacattt acctgacgtt
     6601 tggcacatcc attgtgtcaa aatcacatcg tgacgagctc gggttatctg gctggcaagg
     6661 gccaagaata tcttt
//
LOCUS       DAOSAI010000088         6659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7394_length_6659_cov_4.274228, whole genome shotgun sequence.
ACCESSION   DAOSAI010000088 DAOSAI010000000
VERSION     DAOSAI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7394_length_6659_cov_4.274228"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PLM83_04370"
     CDS             <1..906
                     /locus_tag="PLM83_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HOK70676.1"
                     /translation="FVARTLAKKYNPVVVTMGRGGPAEPELLSASEIEITPEYLLSVS
                     KQGRHASSDHFEDLLTSRVTTIGCRRCGGGMSGQTFVSNVDRGAELSEKIDADIVIFE
                     GSGSSIPSVHTDARILVIGAHQPVEYMRSYLGPYRILTSDLIVLTMCESPMVDQAKID
                     EMVEAIHEINAGCTVVKTIFRPRPIGDITGKRIVLTLTTPAVMTDAISAYLEKTYGCE
                     VVGVSSHLSNRPLLREDLARFETLKPDAIVSELKAAAVDVVTEWAVRKGLEIVYIDNE
                     PIPTDASIDLEEEVLKVARKIKLHD"
     gene            984..1964
                     /locus_tag="PLM83_04375"
     CDS             984..1964
                     /locus_tag="PLM83_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK70677.1"
                     /translation="MKRGSVIVLALVLLLSISTGVFAESKVLLYTSVPLELATNFADQ
                     FMKANKGIKVEVFRAGSTDVVNKIWAEADAGGIRADVIWLADAASYYAFKDKGLLYPY
                     KSPEAARIPSAIKDPDGYFTGARMINMVIGINTNLVKKGSEPKTWDDLLGFGSRAAMA
                     DPLYSGSNFTVAAAFVQRDKGWSWYEKARKSGIQVLRGNSDVSRGLAAGEFAACMVLD
                     YMIYDLQKSGAPVDFVWPADGAVSIPSPVAIVAGSKNLEASKAFVDFTLSKKGQELLV
                     NDGVIPVRPDVAPPKGMPTADKIKMMPVPFQWAADNASAIRETFEKIMFK"
     gene            2087..3766
                     /locus_tag="PLM83_04380"
     CDS             2087..3766
                     /locus_tag="PLM83_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOK70678.1"
                     /translation="MNTDTAVGRRSMDVRYVFMAIIGLFFLVVIVYPLSLVVVKSFSS
                     EAGFTFENYVKIFSESANLKAFWNSIWVSSLSTLICVAVGTILAMLVVRTDIPGKSWL
                     RMLLLLPYGIPPFIGAMAWAQLFGPVGYVSKLYMTLTGATSAPWNIYSAAGVVLVLAV
                     YQFPVVFITVSGALGKIDTSLEEAARMSGAGPFRTLIDVTMPLITPAITASALLAFVG
                     CISNFGIPALLGFRARFFVLTTKIYSALSIPDMPLATAMAVILAITSGIPLILERRLE
                     RGQSRYTVIAGKSVRPQTMSLGGWRIPVFIIVVLVSLFAGVVPLISMLLTSFLKYWGA
                     PLNLANMTTSHYKYILDLPMASRAFRNSLFLAVSAATITMAVGALVSYMSVRAKIKGA
                     KTLDFVGTIPYAVPCTMVAIAMILAWSRPPVMLYGTIWVILAAYLVRYMPFSIRTTNA
                     TLQQVHVSLEEAARVSGAGWTQTMRDIVMPLIKPGLIAGWILVFMPALRELTMSVLLW
                     SQGAETIGVVIYNMQDAGYTQIAAALSTIVLLVILAGNAIVRKLSKGEISM"
     gene            3789..4844
                     /locus_tag="PLM83_04385"
     CDS             3789..4844
                     /locus_tag="PLM83_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70679.1"
                     /translation="MEERIKVSNLVKRFGTFTAVHDVSFTVHENEFFSLLGPSGCGKT
                     TTLRCISGLEQPTEGEIHISGQLVTSPEKNVMVPPERRGIGMVFQNYAVWPHMTVEQN
                     MSYPLRLAKRPKEEINAKMAYLLDLLELEGLERRFPSELSGGQQQRVALGRALSTDPR
                     VMLLDEPLSNLDAKLREQMRFELKDIQRKTGISILYVTHDQVEAMAMSDRVAVMNSGK
                     VVQEGAPHDIYETPKDKFVADFIGTMNFVPCSVTGKTEKGIDIRLRGGAEFGLDGSSV
                     NKGDYILAIRPEDIILGTEQGVECEVDVRIFLGNVIEYRVRIGEDLLRVQTNSSMRFE
                     PGDRTRAFIQNGLVFPK"
     gene            4890..6473
                     /locus_tag="PLM83_04390"
     CDS             4890..6473
                     /locus_tag="PLM83_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70680.1"
                     /translation="MQTTASLQQALRECYSRGEMDYWMTPLTRVVDGKPVGRIKNGDS
                     LFFCCRRGDRERQLTEAFADPLFDQFETEPMPDLSVVPLVQYHEKFAELPTIFPTLRP
                     EGTMGEALSQAGVAQTRIAESEKAAHVTFFFNGRRVAPYANEEWITVPSPHSSEFVSK
                     PATSTVEVALEAERSMAAANASGYSRHFILVNLAAGDIIGHLDDWNANVLCAEAVDQA
                     LSTICLAAAQHGYAAAVTADHGLLERFVNEDGSHNLSHTCSRVPFGLVLPTGGPSVQL
                     RAAEDATLADVAPTLLRMMGVPVPEEMTGKCRAVHEGTYDRLVLVILDGWGVGMDDST
                     INPIAVANTPCMSRMRKECPYMTLAAAGMAVGLPKGRSGNSETGHLTLGAGRVVPQDE
                     LRISQAVGAGGLKENEAVNYGFEAARSHGGDIHVIAMLSERSSHGNMDEGIAVLRAAA
                     GKGIGRAWLHLILDGRSSPPQGAADLLEILEAKIPSGMNVEVVTAMGRAYALDRSGSY
                     QEKTEVAYRALVMGEGRDF"
BASE COUNT         1525 a         1529 c         1936 g         1669 t
ORIGIN      
        1 tttgtcgcgc ggacgctggc gaagaagtat aaccccgtcg tggttaccat ggggcggggt
       61 ggtcctgcag agcctgagct gctcagcgcg tctgagattg agatcacgcc cgagtatttg
      121 ttgtccgtta gcaaacaagg gcgccatgca tcgtctgacc atttcgagga tctgcttacc
      181 tcaagggtta caaccatcgg ctgtcgacgg tgtggaggcg ggatgtcggg acagactttt
      241 gtctcaaatg tggacagagg tgctgaactg tcggaaaaga tcgacgccga catagtcata
      301 ttcgaaggca gcggttcatc tataccttcg gtgcacacgg atgcgcgcat actagtcatc
      361 ggtgctcacc aacccgtaga gtatatgcgc tcttacctcg ggccttatcg tattcttacc
      421 tccgacttaa ttgttcttac aatgtgtgag tctccaatgg ttgaccaagc taagattgac
      481 gagatggtag aagccattca cgagataaac gccggatgca cggtggtgaa gaccattttc
      541 agacctcgcc cgattggcga tattaccggt aagagaatag tcctcactct aactacgcct
      601 gcggtgatga cagatgccat atctgcttat cttgagaaga cttatggatg tgaggttgtt
      661 ggagttagct cgcacctttc gaacaggccg ctgctccgag aggacctggc gcgttttgaa
      721 acgttgaagc ctgatgcaat cgtctctgaa ctcaaggctg ctgcagtaga tgttgtgact
      781 gaatgggcgg tacgtaaggg gcttgagatc gtgtatatcg ataatgagcc tattcctaca
      841 gatgcttcta tcgatctaga agaagaagtg ctaaaggtgg ctcgaaagat caagttgcat
      901 gactagttga ttcgctggtc gtgcccctgc gcgagtgggg gttaaccaag tataccacca
      961 atcaaaagag aggcgggttg aacatgaaaa ggggcagtgt aatagtcctg gctttggtat
     1021 tgttgctgtc gatctctact ggtgtatttg ctgagtcgaa ggtcctgctt tacacgtctg
     1081 ttccactgga actcgctacg aacttcgcag accaattcat gaaggcgaat aaagggatca
     1141 aggtggaagt ttttcgagct ggaagcactg atgtggtcaa caagatatgg gctgaggctg
     1201 atgctggcgg tataagggcc gacgtaatat ggctggctga tgccgcaagc tactacgcat
     1261 tcaaggacaa gggcttgcta tatccgtaca aatcgccgga agccgcccgc attccttcag
     1321 caatcaagga ccctgacgga tactttacag gcgctcgcat gattaacatg gtaattggaa
     1381 taaacaccaa tttagtcaag aaaggatccg agccaaagac ttgggatgac ttgctcgggt
     1441 ttggttctcg cgccgcaatg gcggaccccc tctattctgg ttccaacttc actgttgcag
     1501 cggcttttgt tcaaagagac aaaggttgga gctggtacga gaaagcgcga aaatctggaa
     1561 tacaggttct caggggcaat tcagatgttt cgagaggttt ggctgctggc gaatttgcag
     1621 cctgcatggt gctggactac atgatttatg accttcagaa gtcgggagcg ccggttgatt
     1681 ttgtctggcc tgccgacggt gcggtctcaa ttccgagtcc ggtagcgata gttgctgggt
     1741 caaagaatct tgaggcctcc aaggcatttg tagacttcac tctctcgaag aaggggcagg
     1801 agctattggt caatgatggt gtgataccag tgaggccaga tgtagctcct ccgaagggaa
     1861 tgcctacggc agataaaata aagatgatgc ccgttccatt ccaatgggcg gctgataacg
     1921 cgtctgctat aagggagacc ttcgagaaaa taatgttcaa ataggtgcgt gtcgtgaaca
     1981 aacaccctga ccgcggcggt gcgactgagg ccgcaccgcc tctttctggg gagtacatta
     2041 ctgtatgcag tgtaccccag cccattgtag gagggggatg attcgattga atacggacac
     2101 agcagttgga cgacgatcta tggatgtacg ctatgtgttc atggccatca ttgggctgtt
     2161 cttcctggtc gtcatcgtgt accctctgtc ccttgttgta gttaagagtt tcagttctga
     2221 ggcgggtttt actttcgaaa actatgtgaa aatcttctct gagtcagcca atcttaaagc
     2281 cttttggaat tccatctggg tttcgtctct gtctactctg atatgtgtgg ctgtaggcac
     2341 tatcctggct atgctcgttg tccgaacaga tatcccgggc aagtcgtggt tgaggatgct
     2401 gcttctgctg ccgtatggca taccgccgtt cattggtgcg atggcgtggg ctcagctttt
     2461 tggaccagta ggttacgtat ctaagctata tatgacgctt acgggcgcga catctgctcc
     2521 gtggaacata tacagtgctg ccggggtagt attagtgctc gctgtatatc agtttcccgt
     2581 cgttttcata actgtgagtg gggcgcttgg caagatcgat acgagcttag aagaagccgc
     2641 gaggatgtct ggcgcaggtc cgtttaggac tctcatagat gtaactatgc cactgatcac
     2701 gcctgcgatc acagctagcg cattgcttgc gtttgtcggc tgcatatcca acttcggaat
     2761 tccggctctt cttgggttca gggcccggtt cttcgtgttg actacgaaga tttactccgc
     2821 tctgtctata ccggatatgc cattggcgac tgcgatggcg gttatcctgg cgattacatc
     2881 tgggattccc cttattctcg agaggagact ggagcgtggg caaagcaggt atacagtgat
     2941 tgcgggcaag agtgtcaggc ctcaaacaat gagtctaggc ggttggcgga tccctgtctt
     3001 tatcatcgtt gtcctcgtat cgctttttgc aggggtagtt ccgctgatat cgatgctttt
     3061 gacttcattc ctcaaatact ggggcgcacc gctgaacctt gcgaatatga ctacgagtca
     3121 ttacaaatac attttggatc tgcccatggc atccagggca tttaggaaca gcttgttcct
     3181 ggcggtttct gctgccacaa tcaccatggc cgttggagct ttggtatctt acatgtctgt
     3241 gcgggccaag atcaagggag ccaagactct cgacttcgta ggaaccatac cttacgcggt
     3301 gccatgtact atggtggcaa ttgccatgat cttggcctgg tcgcgacccc ctgtgatgct
     3361 ttacggcacg atatgggtca tacttgcggc ttacctagtg cggtacatgc cattttcgat
     3421 tcgcactacc aacgcgactc ttcagcaggt gcatgtttcg cttgaggaag ctgcgcgggt
     3481 ttccggggca ggatggactc agacaatgag agacattgtg atgccgttga tcaaacctgg
     3541 cctcatagcc gggtggatcc tggttttcat gccagctctg agggagttga ccatgtccgt
     3601 tcttctgtgg tcacagggcg ctgagactat aggagtggtt atatacaaca tgcaagatgc
     3661 cgggtacacc cagatagctg cggcgttgtc tacgattgtt ttgcttgtga tcttggctgg
     3721 caacgcaatc gtgcggaagc tttcaaaagg cgaaatcagt atgtgaacgg tatgcggagg
     3781 tggcaagggt ggaagaacga atcaaggttt caaacctagt caagcggttt gggacgttta
     3841 cagcagttca cgatgtgagc tttacagttc atgagaacga gttcttctcg cttctggggc
     3901 cctctggatg cgggaagacg acaacactcc ggtgcatttc agggttggag cagcccactg
     3961 agggcgagat acacataagc ggtcagttgg taacttcgcc ggagaaaaac gtgatggtgc
     4021 cgcctgagcg ccggggcata ggaatggtct ttcaaaacta cgcagtatgg ccgcatatga
     4081 cagtagaaca gaacatgtcc tacccccttc ggctggcgaa aaggccaaaa gaagagatca
     4141 atgctaaaat ggcttacttg ctagacctac ttgaactcga gggcttggaa agaaggttcc
     4201 catcggaact ctccggtgga cagcagcaac gcgttgcatt ggggagagcg ctttctaccg
     4261 acccaagggt tatgctgctt gatgagcctc tttccaatct cgatgcgaaa ctgcgagagc
     4321 agatgcgctt tgagctcaaa gacattcagc gcaagacggg aatatccatt ctgtatgtta
     4381 ctcatgacca ggttgaggca atggccatgt cagaccgggt tgccgtaatg aactccggca
     4441 aagtagtgca ggagggtgcg cctcatgaca tatatgaaac gccgaaggac aagttcgttg
     4501 ccgacttcat agggactatg aacttcgttc cctgcagtgt gactggcaag actgaaaaag
     4561 gcatcgacat tcgccttcgt ggcggggcgg agtttggtct tgacggatca tcggtgaaca
     4621 aaggagacta tatcctcgct attaggccgg aggatatcat cctggggacc gaacaagggg
     4681 tcgaatgcga agtggacgta aggatattcc tcggaaatgt catcgaatac cgggttcgaa
     4741 ttggcgagga tcttctcagg gtccagacga attcctcaat gaggtttgag cctggcgacc
     4801 gcacccgtgc gtttatccag aacggcttgg ttttccccaa ataacattcg ctgcttagag
     4861 cagacaacga agtgcaggag tgacatggag tgcagacaac agcttctttg cagcaggctt
     4921 tgagagagtg ctacagccgg ggagaaatgg attactggat gactccactg actcgcgtcg
     4981 tcgatggcaa gcctgtgggc aggataaaga atggcgattc tctattcttt tgttgcaggc
     5041 gtggggaccg tgaacgacag ctcacagagg ccttcgctga cccgctattt gatcaattcg
     5101 aaacggaacc catgcctgat ctgagcgttg ttccgttggt gcaatatcat gagaaatttg
     5161 ctgagttgcc cacaatcttt ccgactcttc gtcctgaagg aaccatgggt gaggcgctga
     5221 gtcaggccgg cgtggcgcaa acgcggattg ccgagtcgga gaaggcggcg catgttacgt
     5281 tctttttcaa tggcaggcgc gttgcaccgt atgccaatga agagtggatt actgtgccaa
     5341 gtccccactc atcagaattt gtctcgaaac cggctacgag cacggtggaa gttgccctcg
     5401 aagctgagcg ctcgatggct gctgcgaacg ccagtggtta tagcaggcac ttcatccttg
     5461 tgaacctagc ggcgggggac atcattgggc atctggatga ttggaatgct aatgtgctct
     5521 gtgctgaagc ggtagatcaa gcgctcagca ccatatgcct agctgcggct caacatggtt
     5581 atgcagctgc tgtaactgca gatcatgggc tgctcgagag gtttgtgaat gaagatggga
     5641 gccacaacct tagccataca tgtagtcgtg tgccgttcgg cttggtgttg ccgaccggag
     5701 ggccgtctgt gcaactgagg gcggcggagg atgccaccct tgcagatgtt gcgcccacgc
     5761 tgttgagaat gatgggcgta cctgtgcccg aggagatgac cggcaagtgc cgcgccgtcc
     5821 atgagggaac ttatgatcga ctggtattgg ttattttgga tggatgggga gttggcatgg
     5881 atgattcaac catcaacccg attgcagttg ccaacacgcc gtgtatgagc agaatgagga
     5941 aggaatgtcc atatatgact ctggctgcgg ctgggatggc cgttggtttg ccaaagggaa
     6001 ggtcaggcaa ttcggagacc gggcatctaa ctctcggcgc cgggcgggtt gttccccaag
     6061 acgaacttcg gatttcgcag gctgttggcg ctggagggct caaagagaat gaggcagtca
     6121 attacggctt tgaagcggcg cggagccatg gcggcgatat tcatgtaata gctatgctgt
     6181 cggaacggag ctctcacggg aatatggacg aggggattgc agtgctcaga gcggcagcgg
     6241 ggaaaggaat tggacgggct tggctccatc tgatccttga tggtcgaagc agtccgccgc
     6301 agggtgctgc tgatctacta gagattcttg aggcaaagat cccgtcaggg atgaatgtag
     6361 aagtggtaac ggcgatgggg cgtgcatatg ccttggatcg cagtggaagc tatcaggaaa
     6421 agacagaggt tgcctacagg gctctcgtta tgggagaggg ccgcgatttc tagcttaacg
     6481 tccgcctaac ccgtaaatgt tgagaacagc agaatgatgc cggctattga atgtgagggc
     6541 aggggcaatc cctgccctcg gcgacattat tgagtcgtgt atccgtgctg ttgacagact
     6601 aatcattgtc ttgctgtgcg aaccgttctt tccgctctgg gttattgttc tacctcatc
//
LOCUS       DAOSAI010000089         6640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7416_length_6640_cov_3.070159, whole genome shotgun sequence.
ACCESSION   DAOSAI010000089 DAOSAI010000000
VERSION     DAOSAI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7416_length_6640_cov_3.070159"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PLM83_04395"
     CDS             <1..811
                     /locus_tag="PLM83_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOK70681.1"
                     /translation="SKRPADVDHPYGHGKAEPVAAKIVGLILIIAGCAIGSNSARKIA
                     VGGYTEPGKIALVAAVVSIVVKEAMYRITAATAEAIESSAVMAEALHHRSDAYSSVGT
                     FIGILGARLGVPVLDPIAGIVVAALIVKMGFEITRKSSDELMDAQADPDTLRAVQEAI
                     DSVEGVSDVHYMAARRYGGYAHVDLRIGVDPSLSVVEGHNIAVAAEKAIKGVCPHVIG
                     VMVHVDPESVHEDDPIGHGYADVFQPEGPQRIAQPNAAEMTSVVEPSHIRE"
     gene            942..1709
                     /locus_tag="PLM83_04400"
     CDS             942..1709
                     /locus_tag="PLM83_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 family protein"
                     /protein_id="HOK70682.1"
                     /translation="MRLFLSRKQHMFALMVACIACAILAYAGVVGDNEMPASQGAADF
                     ISAVDMPAAGAWDVLSELNMVGVSNVEGPVIAIARECVALSVRGQPVPSATELIMSVS
                     AIPEVEQALTRRSAGVFVTVVRNNSVRACVGSIWPRCANLAEEIAQSAREVAARDLRR
                     PPIEPWELPTCEYAVSVVGRLERVSPDANWDPRAFGVFVRAGHRSGVILPGEALTHSK
                     QLSWARDEAGIEPDEECEIYRFQTVKFGCSLDLGRGR"
     gene            1728..6287
                     /locus_tag="PLM83_04405"
     CDS             1728..6287
                     /locus_tag="PLM83_04405"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOK70683.1"
                     /translation="MRCLGRSARVSHAIVLAALLALFMAPAAMSAREPQVWLFATARN
                     YLPENEPELGIRGYGVKELWLDIYKFDGFRHFKNGDWSALWNVNYKDLPGREKVRSVR
                     VDIARPGGTLDAAVKLKPLPPGQYVAVARSGQLKTEEAAWFAVSELGLISKQYSRGLV
                     IYAVNLRTGEPMPNVSVYAKLSSMSGSDESERFDQETVESGEKGDIGGAAVVSVGSVS
                     VPGEVYGMTDNRGLFTIDLADPADSAFVVAAKGDDFAVLNSSYWWDARTQKVYVYTDR
                     PVYRPNNVVYFKGIARGITNSGYEVLARRPVRVEVRDPRNNVVYKQDLLTNDWGSFSG
                     EFKLGAEPPLGHYWICAIIDGEQHEGSFQVAEYRKPEWVVEIEFDKEMYIAGDRLDAT
                     CKALYYFGAPVRNARVAYRVYRQPMGFFGPTAEDSGYDYDQYGGVDDMVGLYYGDFVL
                     SGETRTDSDGLARISFESPAADGHNNRYIVEADVIDATNKRAYGRGYTDVARGTIDVD
                     VSTDRYVVREGDKFEVYAKAVDLDGNGVVRDIDIIVLKRSYSKKGYTDTPVHSTQITT
                     AASGDGFIELEAGKPGDYVIQAKSEDERGNAIIAETYIWTTPDNGSWADMFRTDLGIV
                     CDRGRYDVGDVAKVLITAPPEVSSVLLTVEDREVRDVRVIRLENGSAEVELAIKPEYA
                     PNTYVSVVAIVSGVMQTAARQIVIPVPERLLNVEIKPNKDSYRPGETATYLIVTENSD
                     GDGVPAEISFGLVDESIYAVRPDTTPAIDKFFHGRRERAVSTENSFPVTYYGGAEKEG
                     GTEATRKYFPDTAAWFPSVVTDSNGHAVVRVKMPDSLTTWRATVRAHTRSTIVGQAQE
                     KVTVSKPLSIRRGIPRHYTMGDCGRVTGVIHNETKWQRIVFVNLNASGAKIVGRSWQW
                     VKVPASGEAVVEWEVEPETVGDVVFKANAWSWFVGDKLALSVPVLPFGEKCDEVWAGE
                     VRPDGGRGEKDIEFDLPDDIVQGATSLSVDISPGYAGVVESALEFLVNYPYGCVEQTM
                     SAFLPDILAHRAFACMGIPMPRTEEELADMVNSGLARLYKFQHWDGGYGWWEHDDSGL
                     WMTSYVLYGLERARQSGFCVSEEAMARAADYLREAVWDEKSDTNRAFACYVLAEQGAL
                     GAKGMKALEQLAAKLPAYPNVEHGRRVAAESQSLKPLTVAYVTLAASAAGRDDMASAG
                     AETLRNMATVISGQAFWETEDKENWWRNETGETTAWAMMALLEAEPSSPLLSQAARHL
                     AMTRDGSQWRSTRESAAAIMALVEYMTAMEEDVRQRRQVTVEVNGRRIAKEYLGGDSG
                     YEAANLCVKIDPEFLIVGRNSVKISAEGGPVYYSINASWHEARDRIEAAGECADIVRT
                     YFVIDRNAEAPEGEEYALIPVDEDTPVMCGQELLVRVAIDAKRDMDYMIFEDPVPSGF
                     EISEGFTDPWSWNYWYDRREVRDDRMVFFATWIKAGESMVFDYILRPERLGRYMVMPT
                     RAWSMYYPGLRANGASRVIEVADGE"
     gene            6284..>6640
                     /locus_tag="PLM83_04410"
     CDS             6284..>6640
                     /locus_tag="PLM83_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOK70684.1"
                     /translation="MRRQWVAVSIAVGVIAASVLLCASGGRAPSETTPVNKATGHVLG
                     RCITLLNADEKERNANVALGARKLDGTVIDAGTEFSFNASIGERGVEQGYKPAMVIVD
                     GKNESGIAGGICQLSST"
BASE COUNT         1521 a         1535 c         2071 g         1513 t
ORIGIN      
        1 atcgaaaagg cctgccgatg tggatcatcc ctacggtcac ggcaaggctg aaccggtagc
       61 cgccaagatt gttggtttga tcctgatcat tgcagggtgt gccatcggat cgaattcggc
      121 taggaagatc gcggtgggcg ggtatacaga accgggcaag attgcccttg tggccgccgt
      181 ggtgtcaata gttgtaaagg aagccatgta tcgcatcacg gctgccactg cagaggcaat
      241 agagagcagc gcagtcatgg cggaagcact gcatcaccgc tctgatgcat attcctcagt
      301 tggaacattc ataggcatcc tcggcgcccg acttggagtg cctgtgctcg atcccatagc
      361 cgggatagtg gtagctgccc tcatagtcaa gatgggcttt gagatcacga ggaagtcttc
      421 tgacgagctc atggatgcgc aggctgatcc ggacacgctc cgtgctgtgc aggaggctat
      481 cgattctgtg gaaggcgtaa gcgacgtgca ttacatggcc gcgaggcgct atggaggcta
      541 tgcgcacgta gacttgcgca taggagttga tccttctttg tcggtggtgg agggacacaa
      601 catagcagtg gcagctgaga aggccatcaa aggcgtgtgt ccacacgtaa taggggtcat
      661 ggtacatgtg gatccggagt ctgtccacga ggatgatccc attggtcacg gttatgccga
      721 tgtctttcag ccggaaggcc cgcagcgaat cgctcaacca aatgctgcag aaatgacaag
      781 tgtggttgag ccaagccata ttcgggagta agtgtcttat cgccgccccc tctcttgagt
      841 aggactgatg ctctcatctg tcgaatacgc ttagagaggg gcgcggcact gtcccatcca
      901 ggcaatgccc ttcatgctct cctcttaaag ggtgtcttcg aatgaggctt ttcctttcgc
      961 gcaaacaaca tatgttcgca ttgatggtgg catgcattgc gtgtgccata ttggcctatg
     1021 caggcgtggt cggcgacaac gagatgcccg cctcccaagg tgccgcggac tttatatctg
     1081 ctgtagacat gccggcggca ggagcttggg atgtactgtc ggagcttaac atggtaggtg
     1141 tctcaaacgt ggaaggccct gtgattgcca ttgcgcggga gtgtgtggcc ctctccgttc
     1201 gggggcagcc tgttccatca gcgaccgagc tgatcatgtc ggtcagcgcg attccagagg
     1261 tggaacaggc tttaacgcgt cgttctgcag gggtttttgt taccgttgtc aggaacaact
     1321 ctgttagggc ctgcgtcgga tcgatatggc caagatgcgc caatctggcg gaggagatag
     1381 cgcagtcagc tagggaggtt gcagctcgcg atctcaggcg tcctcccata gaaccctggg
     1441 agcttccgac ttgcgaatat gcagtctctg ttgtgggtcg acttgagagg gtttcccccg
     1501 acgcaaactg ggatccgcgt gcttttgggg tttttgttag agcaggtcac aggtctggcg
     1561 tgattttgcc tggagaggcc ttgacacact ctaagcaatt gagttgggcg agggatgagg
     1621 caggcataga gcccgacgaa gaatgcgaga tttatagatt ccagacggtc aagttcggtt
     1681 gctcattgga tttgggacgt ggccgatagg gcttggaggt aatgattatg cgttgtctag
     1741 gaagatcagc ccgcgttagc catgcaattg ttttggccgc gcttcttgcc ttgtttatgg
     1801 ctccagcggc gatgagcgcg cgggaaccgc aggtgtggct ttttgcaacg gcaaggaatt
     1861 acttgcccga aaacgaacct gaactcggaa tcaggggcta cggtgtaaag gaactttggc
     1921 ttgatattta caaatttgat ggcttccggc acttcaagaa tggcgattgg agtgctctgt
     1981 ggaatgtgaa ctacaaggat ctgccggggc gggaaaaggt gcgctctgtc cgtgtcgaca
     2041 tagcgaggcc aggcggcacg cttgatgcag ccgtcaaact taaacctctg cctccaggac
     2101 aatacgtggc agttgccaga tccgggcagc tcaagacgga ggaagcggct tggtttgccg
     2161 tgagcgagtt ggggctgatc tcaaagcagt actcgcgggg gcttgtaatc tatgccgtca
     2221 acctcagaac aggcgaaccc atgcccaacg tgtcggtata tgctaaactc tcgagcatga
     2281 gcggttctga tgagtctgag cgttttgacc aagagacggt ggagtctgga gaaaagggcg
     2341 atatcggcgg ggctgctgtg gtgagtgtcg gctccgtttc tgttccaggc gaagtttacg
     2401 ggatgacaga caatcgaggg ctattcacta tagacctggc cgatcctgca gattcggcct
     2461 ttgtagtggc agcaaagggc gatgacttcg ccgtgctcaa ctcgtcttac tggtgggatg
     2521 cacggacgca aaaggtttat gtgtatactg atagacctgt ttatcggccg aacaacgtcg
     2581 tttatttcaa gggaatcgcc cggggcatta ccaattcggg ttatgaagta ctggctcgtc
     2641 gacctgtcag ggtagaagtg cgcgaccctc gcaacaatgt cgtctataag caagatttgc
     2701 tcaccaacga ttggggttct ttctcgggcg agttcaagct cggagctgaa ccgccattag
     2761 gccattactg gatatgtgca attattgacg gagagcagca tgaagggagc tttcaggtag
     2821 cggagtatcg aaaacctgaa tgggttgtgg agatcgagtt cgacaaagag atgtacattg
     2881 ccggcgatcg tctcgatgcc acgtgcaaag ctctttacta ttttggagct cctgtaagga
     2941 atgcccgggt ggcctatcgt gtctatcgcc agcccatggg ctttttcggc ccaactgcgg
     3001 aagattcggg ctacgattat gaccagtatg gcggcgtaga tgacatggtt gggttgtatt
     3061 atggggattt tgttctttca ggcgaaactc ggaccgactc agatggctta gcgcggattt
     3121 ccttcgagtc acctgcagcc gatggccata ataacagata tatagtggaa gcagacgtca
     3181 ttgacgcaac aaacaagagg gcatatggaa gaggatacac cgatgttgcc agaggcacaa
     3241 ttgacgtcga cgtttcgact gaccgttacg tagtccggga gggagacaaa tttgaagtat
     3301 acgcgaaagc ggtcgatctc gacggcaacg gagtggtcag ggacatcgat attatcgtgc
     3361 tcaaacggag ttacagcaag aaaggctaca ctgacacgcc tgtccactca acccaaatca
     3421 caactgctgc atcgggcgat ggcttcattg agctggaagc cggaaaaccc ggagattacg
     3481 taattcaggc caagtccgag gatgaacgtg gcaatgccat aatcgcggag acctatattt
     3541 ggacgactcc ggacaatgga tcgtgggccg acatgttccg gactgacctg ggtattgtgt
     3601 gcgaccgagg cagatacgac gtaggcgatg ttgcgaaggt tctgattact gcgccgcctg
     3661 aggtaagttc tgtccttctt acagttgagg acagagaagt tcgggatgtg cgcgtcataa
     3721 ggctcgagaa cgggtcagca gaggtggaac tagcgattaa gccggaatat gccccaaata
     3781 cgtatgtgag tgttgttgca atagtgtcag gtgttatgca gacagcggcg cgccagatcg
     3841 tgatccctgt tccagaacgt ctactcaatg tcgagataaa gccgaacaaa gactcgtacc
     3901 gtcccgggga gactgcaacg tatctgatcg tgactgagaa ttccgatgga gatggtgttc
     3961 cagctgaaat atccttcggt ttggtggacg aatcgatcta cgcggtcagg cccgacacca
     4021 cgcctgcaat cgacaagttt ttccacggac gtcgcgaacg ggctgtctca acggagaact
     4081 cttttccggt gacttattac ggcggcgccg agaaagaagg cggcacagag gcgacacgca
     4141 agtacttccc tgatacagcc gcatggtttc cgtctgttgt gacagacagc aatggtcatg
     4201 cagtggttcg ggttaagatg cctgactcgc ttaccacgtg gcgagctacg gtaagggcac
     4261 atacgcgttc caccattgtt gggcaggccc aggaaaaggt gaccgtgagc aaaccgcttt
     4321 caataagacg tggaattccg cgccactata ccatgggcga ctgtgggcgt gtaacggggg
     4381 ttattcataa cgagaccaag tggcagcgga tagtcttcgt gaacctcaac gcgagcggcg
     4441 ccaagatcgt gggccgctca tggcagtggg tcaaagtgcc tgccagtgga gaagctgtgg
     4501 tggaatggga ggtagagccg gagaccgtgg gcgatgtggt cttcaaggca aatgcctggt
     4561 cctggtttgt tggagacaaa ctcgcgttgt ctgtcccggt tcttccgttt ggagagaagt
     4621 gcgatgaagt ctgggccggc gaggtgcgcc ctgacggagg gcgcggggag aaggacatcg
     4681 agtttgattt gcccgatgac atagtgcagg gagctacgtc gctatcagtg gatatctctc
     4741 cagggtacgc tggtgttgtg gagtctgccc tcgaattcct ggtcaattac ccgtatggat
     4801 gtgttgaaca gacgatgagt gctttcctcc ccgacatatt ggcacaccgc gcttttgctt
     4861 gtatggggat ccccatgcca aggactgagg aggagcttgc cgacatggtc aattccggac
     4921 ttgcgcgcct ctacaagttt cagcattggg atggtgggta cggttggtgg gagcacgacg
     4981 acagtggcct atggatgact tcatacgtgc tctacggctt ggaaagggcg aggcagagcg
     5041 ggttctgtgt aagtgaagag gcaatggcaa gagccgccga ttacctgagg gaagcagtat
     5101 gggatgagaa gagcgatacg aaccgcgcat ttgcctgtta cgtcctggcg gagcaaggcg
     5161 ccttgggcgc aaagggcatg aaggcccttg aacagcttgc cgcgaaactc ccggcgtacc
     5221 ccaatgtgga gcatggaagg cgggttgcag ctgaatcgca atcgctgaaa ccattgactg
     5281 ttgcatatgt aacactggcg gcgtcggctg caggacgcga cgacatggca tcggcaggcg
     5341 cggagactct gaggaatatg gcgactgtga tttcagggca ggcgttttgg gagactgagg
     5401 ataaggaaaa ttggtggagg aatgaaactg gcgagaccac tgcatgggcc atgatggctt
     5461 tgctcgaggc tgaaccttcc agtccgcttc tatcgcaggc tgcccgacat cttgctatga
     5521 caagagacgg gtcacagtgg agatcaacgc gggaatccgc tgcagcaatc atggcgttgg
     5581 tagagtacat gaccgctatg gaggaggatg tcagacagcg aaggcaagtg acggtggagg
     5641 tcaacgggcg gcgtattgcc aaggaatact tgggaggaga ttcaggctac gaggccgcaa
     5701 acttgtgtgt caagatcgac cccgaatttc taatagtcgg gagaaactcg gtcaagatat
     5761 cggccgaggg aggtcctgtt tactacagca ttaacgcatc atggcatgaa gcccgtgatc
     5821 gcattgaggc ggcaggagag tgcgcagaca ttgtccgcac atattttgtc atagacagaa
     5881 atgccgaggc cccggagggc gaagaatatg cgctgattcc tgtggatgag gatactcctg
     5941 tcatgtgcgg acaggagctc cttgtgaggg tcgccattga tgccaagcgc gacatggatt
     6001 acatgatttt cgaggatcct gttccgtctg ggttcgagat atccgaaggc tttactgatc
     6061 catggtcttg gaattactgg tatgataggc gagaagttag ggacgataga atggttttct
     6121 ttgcaacatg gatcaaagcg ggcgaaagca tggtgtttga ctatattctc agacctgagc
     6181 gactgggacg ctacatggtg atgcctactc gagcctggtc aatgtactat cctggattaa
     6241 gagcgaacgg ggcgagcagg gtgatcgagg tggctgatgg ggaatgaggc gtcagtgggt
     6301 ggccgtatct atagctgtag gtgtcatagc tgcatctgtt cttctctgtg catccggggg
     6361 ccgtgcgcca tctgagacca cgcctgtcaa taaagcaacc gggcacgtgc tgggacgctg
     6421 tatcacgctg ctcaacgccg atgagaagga gagaaacgcc aacgtggccc ttggcgcaag
     6481 gaagctcgat ggaacggtta ttgatgccgg aacggaattt tccttcaacg cgtccatagg
     6541 ggagcgcggc gtcgaacagg ggtacaaacc tgccatggtt attgtagatg gcaagaatga
     6601 gtcaggcatt gcaggaggca tatgccagct atcatctacg
//
LOCUS       DAOSAI010000090         6590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7473_length_6590_cov_3.593420, whole genome shotgun sequence.
ACCESSION   DAOSAI010000090 DAOSAI010000000
VERSION     DAOSAI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7473_length_6590_cov_3.593420"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLM83_04415"
     CDS             complement(<1..254)
                     /locus_tag="PLM83_04415"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOK70685.1"
                     /translation="MLMSMTGYGRAANSGPEGDVTVEIRSLNHRYFDVSLRMPREFSP
                     VEERVRSLVAERIARGRIEVSVSVDWHDGSRRTVALDTCLA"
     gene            complement(426..3248)
                     /locus_tag="PLM83_04420"
     CDS             complement(426..3248)
                     /locus_tag="PLM83_04420"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOK70686.1"
                     /translation="MSELRAWHSVSAGVVERELETSASSGLTSKEARARRGAYGPNQI
                     RAGRRQSPLVILLNQFQDFMVLVLLGATAVSAFLGEYRDVVAILAIVVLNAVLGFVQE
                     FRAEKALEALRKLSAPKATVLRDGRATQIDAVDVVPGDVIILNPGDRIPADARLAESY
                     GVEVDESVLTGESRPVKKDASCIVRQSAPVVEHRNMLHAGTVVTRGRAIGLVVATGME
                     TEMGRIAGMIEDVSDGDTPLQRRLSHLGRWLVAGCLFLCALVAGLGVARGEPIELMFL
                     AGVSLAVAAIPEGLPAIVTVSLALGVQRMSARKAIVRKLSAVETLGCATVICADKTGT
                     LTQNQMTVVEIDLIGRNIQVTGSGYSPEGEFLEGGETIDPLNDGIFEDLLLCAALCND
                     ASLVRQSAEERTTGSMGRGRGRGYGYGPGRSWDVVGDPTEGALLAMAYKGGREIMQKV
                     RSFTRVAEIPFEPERRMMAVACSGPSGVFTFVKGAPQVVLELCEGRYVGGHVEGGIVL
                     RDRRFDGAVLARNSSMASKSMRVLAMASAPGNLIEQIRVGQSHALSGLTFLGMVGMVD
                     PPRPEAARSVALAKKAGIATLMITGDHAATAEAIAKQVGIMESGRRILTGEELDAMSD
                     RQLEEVISEVAVFARVAPAHKLRVVRALRAQGHVVAVTGDGVNDAPAIREADIGIAMG
                     KNGTDVAREASSMVLSDDNYATIIAAIEEGRSIYDNIRKFIRYLLACNVGEVLTMLLA
                     VAGGLPLPLTPIQILWMNLMTDGLPAMALAADPTDPNTMQRAPRCPSESLFAHGLGLK
                     VLADGVFISICTIACYISAGAFLGHDLDTARTMAFSTLVMSQLVYVFRCRVEERSALE
                     SGRRPSNRFLLGAVGISVVLQVLGVHHHVGRAFLGTCELSVVDWVLVLFLSGWSTFLA
                     SAVRAVQRAIRRRCTVFRVNKAGQGT"
     gene            3375..5258
                     /locus_tag="PLM83_04425"
     CDS             3375..5258
                     /locus_tag="PLM83_04425"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOK70687.1"
                     /translation="MQIDGMTLYAVTREIERELTGTRIDKIQSPSHDCITLDLWRGRA
                     PSLLISASPTSSRICLTDSVPPSLPTPTAFCMLLRKHLVGGRVVSVRQDGLDRIIIMQ
                     IEGWADTDPSDNKLLIAELTGRLSNIILVRMDGRILDAAHRVDTSRNRYREITPGASY
                     VPPPPIEKADPFRVTAEEFRAIIQRQSAQIRARAEADAASKRRPSRRKDSVANLISSG
                     FAGIGPNHSREIAWRAGIDPDRSALELHADDISLLWAQFEDFRRAVKVGDFLFEASFD
                     RHSGKPTACCVWGLSGLSGEYRVERFASASEMIDRCYNRAEERERLELTRSNITHKVE
                     ARLARSVRKLEAQRRDLAEAEDSSAFRRKGELLTANIHSLGRGPLREDRVRVVDYYDP
                     AAPTVEIEVDPSLSASENAQSFFARYSKAERARKAVTANIAATEEEVAYLESMLAMAT
                     IAETEETLNGITEELAALGFIQPRQGRGSARRAKKKHKERLPMPTSFRAASGDEIYVG
                     KNNLENDHLTTKLARPHDLWFHAKDIHGAHVVVRRRPNQEVPDETLRAAALLAAYYSQ
                     ARMSSNVAVDYTEARNVRKPRGARPGMVIYEAHKTIWVTPSREELSNALGFNGDTCPG
                     NED"
     gene            complement(5193..6485)
                     /locus_tag="PLM83_04430"
     CDS             complement(5193..6485)
                     /locus_tag="PLM83_04430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOK70688.1"
                     /translation="MGGAAQDSGAVSMMGNGGSKKADGTGRVDLAVRRAAALEVLLAV
                     LASCVICTGCAPDCTWLRFSEGRAAYPCSDGSRLEDVWQRTACESLQSRFTLAAAGDV
                     IFDRGVRAVCERRGADWPLSAVAPIFSSADAGFVNLETSLAVGGRPIPGKGIWFRSDP
                     KFAQELAKAGVNVVTVANNHVLDYDEAAFAETLAALADSSIAVCGGGFDLDEARSPAI
                     VSAGGVRIAFLGYSELADIFWTYSYPRSFRAGLDSPGVAPMDEKMIVQDIRKARAVAD
                     HVVVSYHWGEEYARMPNTRQMQIAHMSIDAGASLVLGHHPHVLQPVEVYNGGIIFYSL
                     GNFVFDQTKPDTVESMIARVTFSPSKIVSAQILPMRIEECRPRPLVGWAARTVLDRLI
                     RDSAWLGAVVAPTGGRGLILIPGTSVPVETEGVGEFLP"
BASE COUNT         1520 a         2166 c         1668 g         1236 t
ORIGIN      
        1 gcaaggcacg tatctaaagc gacagttcgc cgactgccgt cgtgccaatc taccgacacc
       61 gagacttcga tcctgccgcg cgcaattctc tccgcaacaa gggatcttac cctctcctca
      121 accggagaga actccctcgg catccggagc gagacatcga agtatctgtg gtttaaagat
      181 ctgatctcaa ccgtgacatc gccttcggga ccgctgtttg cagcccgccc atacccggtc
      241 atactcatca gcatatatca aagttccatc cttttccgtc tagcgtgcag ctcattgtac
      301 tacacaaaca ccagctgcat caagtattca ctgcaaaatg ccagcccaaa cgggatgctt
      361 ttaggaacct agtattccac tcagacaccg aaaatcctgc taggatatgc aatatccctg
      421 cggtttcatg tgccttggcc cgctttattc accctgaaaa cagtacatcg ccgtctgatg
      481 gccctttgta ccgccctgac cgcagaagcc aggaaagtag accagccgga gaggaaaagg
      541 accaacaccc aatccaccac cgacaactcg caggtcccca agaatgcacg gcccacgtgg
      601 tgatggacgc ctaacacttg aagaacgact gagatcccga ccgcaccaag cagaaatctg
      661 tttgaaggcc gcctgcccga ttcaagagcc gacctctcct ccactcgaca tcgaaagaca
      721 tagaccagct gcgacatgac caaagtggaa aaagccattg tccgtgccgt atcaagatcg
      781 tgtccgagaa aagcgccggc cgatatgtag caggcaatgg tgcaaatgct gatgaagact
      841 ccgtccgcga gcactttcag ccccaaaccg tgggcgaaca ggctctcact gggacatcta
      901 ggggctcgtt gcatcgtatt tgggtccgtg gggtcggcag ccagggccat agcaggcagc
      961 ccgtcggtca tcaagttcat ccacaggatc tgtatgggcg tcagcggcaa cggaagcccg
     1021 cccgctacag ccagaagcat ggttagcacc tcgcccacat tgcacgccaa gaggtatctg
     1081 atgaacttgc ggatattatc atagatcgac ctgccctcct cgatcgccgc gattatcgtg
     1141 gcgtagttgt cgtctgacag caccatgctc gatgcctccc tggccacgtc agtcccgttt
     1201 ttccccatgg cgatccctat atcggcctcg cgtatggccg gagcatcgtt aaccccatcc
     1261 cctgtcacag ccaccacgtg accttgcgct ctgagagccc gaactacccg gagtttgtgt
     1321 gcaggcgcca ccctggcaaa gacagctacc tcggaaatca cctcttcaag ttgccgatcg
     1381 ctcatggcgt ccagttcctc gcctgtgaga atccggcgcc cgctctccat gattcccacc
     1441 tgcttcgcta ttgcctctgc agtcgcagca tggtccccgg tgatcatcaa cgtcgcgatc
     1501 cccgctttct tagccagagc aactgagcgc gccgcctccg gacgcggtgg atcgaccatg
     1561 ccgaccatcc ccaggaatgt gaggcccgaa agagcatgag actgccccac ccggatctgc
     1621 tcgatgaggt tccccggcgc cgaggccatg gcgagcaccc tcatcgactt cgatgccatg
     1681 gaggaattgc gtgccagcac ggcaccgtca aaccggcggt cacgcaacac gatgccgcct
     1741 tccacatgtc cccccacata acggccttcg cacagttcga ggactacctg gggcgctccc
     1801 ttcacgaaag taaatacccc ggaaggccca ctgcaagcca ctgccatcat ccgccgctcg
     1861 ggctcgaacg ggatctctgc aacccgtgta aagctgcgca ccttttgcat aatctcccgg
     1921 ccacccttgt atgccatggc aagcagagcg ccttcagtgg gatctccgac aacatcccag
     1981 ctccgtccag gcccatagcc ataaccccgt ccccgtccgc gccccatgga gccggtcgtc
     2041 ctctcctcag cactctgccg cacgaggctt gcatcgttgc acagagcagc acaaagaagc
     2101 aagtcctcaa aaatcccatc atttaaaggg tcgattgttt cgccgccttc caagaattcg
     2161 ccctcagggg agtaccccga ccctgtcacc tgaatattcc gtcctatcaa atcgatctcc
     2221 accacagtca tctggttctg agtcaaagtg ccagtcttgt cggcgcagat cacggtagca
     2281 caacctaaag tctccaccgc cgaaagtttc ctgacaatag ccttcctcgc gctcatcctc
     2341 tgcaccccca gagcgaggct gacggtgact attgcaggca gcccttcggg gatcgccgcc
     2401 acagccaagc tcaccccggc aagaaacatg agctcaatag gttcccctct agcaaccccg
     2461 agcccggcaa cgagagcgca tagaaagaga catcccgcca ccaaccacct gccgagatgc
     2521 gagagcctac gttggagagg agtatcgccg tcagatacat cctctatcat cccggctatc
     2581 ctccccatct cagtctccat ccccgtggcg acaactaagc ctatcgcccg cccccgcgtc
     2641 accacagtcc ctgcgtgcag catgttcctg tgctccacaa cgggcgccga ttgccgcaca
     2701 atgcaggaag catccttctt gaccggcctt gattcgccag tcaggacaga ctcgtccacc
     2761 tctaccccgt agctctctgc gagcctcgca tctgccggaa ttctgtcgcc cgggtttaag
     2821 atgatcacat ctccgggaac cacatccacc gcatcgatct gcgtggcgcg tccgtctctg
     2881 agcaccgtcg ccttgggcgc cgaaagcttg cgcagagctt caagagcttt ttctgccctg
     2941 aattcctgga caaagccgag aaccgcgttg aggacgacta ttgcgagaat ggccacgacg
     3001 tctctgtact cgcccagaaa ggctgataca gccgtggcac cgagaagcac tagaaccatg
     3061 aagtcctgaa actggtttaa aagaatcacc agcggcgact gacgccttcc cgcccttatc
     3121 tgatttggtc cgtacgcccc ccgcctcgcc cgcgcctcct tagacgtgag gccggacgag
     3181 gccgaagtct cgagctccct ctcaaccacc ccagcgctga cggagtgcca agcgcgcaac
     3241 tcggacattg ccaacaccac ctccacaccg tgccatcttc cgcacgagca gtctcgtcct
     3301 ttattcctat tgtcctttga ccgggttcat acacgggtgc tatactgaaa actgcattga
     3361 aaggggcgca gatcatgcag atagatggaa tgacactcta tgccgttact cgggagatcg
     3421 aaagagagtt gaccggcacc agaatcgata agatccagtc gccttcgcac gactgcatca
     3481 cgcttgatct ttggcgtgga cgcgcaccgt cgctcctgat atccgcctcg cccacatcta
     3541 gcaggatatg cctgacagat tcggtccccc cttccctgcc gacccccacc gccttctgca
     3601 tgcttctgcg aaagcactta gtcggcggca gagtcgtatc tgtccgccag gatgggctcg
     3661 accgaatcat aattatgcag attgaagggt gggcagacac cgatccttcc gacaacaagc
     3721 tcctcatagc cgagcttaca ggtcgcttgt ccaacatcat cctcgtccgt atggacgggc
     3781 gtattctcga tgcagcacac cgagtggaca catcacgcaa cagatacagg gaaatcacgc
     3841 cgggagcatc atacgttccg cctccgccca tcgaaaaagc tgatcccttc cgggtgacag
     3901 ccgaagagtt ccgcgccatc attcaaaggc agtccgcgca gatccgcgcg cgagccgagg
     3961 ctgacgccgc gtccaagcgt cgcccaagca ggcgcaagga tagcgttgcc aatctcatct
     4021 catctggctt tgcaggcatt gggccgaatc acagcagaga gatcgcttgg cgggcaggca
     4081 tcgacccgga ccgttcagcc ctcgagctcc atgcggatga catcagccta ctgtgggcgc
     4141 agtttgaaga ttttcggcga gcagtcaagg tgggcgactt ccttttcgag gcatccttcg
     4201 accgtcactc cggcaagcct acagcctgct gtgtttgggg actttcaggg ctttctgggg
     4261 agtatcgcgt agaaagattc gcatccgcct ccgagatgat cgacagatgc tacaatcgcg
     4321 ccgaagaacg cgaaaggctg gaattaacca gaagcaacat cacgcacaag gtagaggcca
     4381 ggctagcaag gtcggtgcga aaactcgagg cccaacggcg ggatctggcg gaggctgagg
     4441 attcatcggc cttccgcaga aaaggcgaac tcctaacagc caacatccac tcgttagggc
     4501 gcggcccatt gagagaagat cgcgtgcgcg tcgtggatta ctatgacccc gcagcgccta
     4561 ccgtcgagat cgaagtcgac ccgagcctgt cagcgtccga gaacgcacag tccttctttg
     4621 cacgctactc caaagcagag agggcccgaa aagcggtgac tgccaacatt gccgccaccg
     4681 aagaggaagt ggcttatctc gaatcgatgc tggccatggc gacaatagcc gaaacagaag
     4741 agacattgaa cggtatcact gaggaactcg ccgccctcgg cttcatacag cccaggcagg
     4801 gacgcgggag cgcgcggaga gcgaaaaaga aacacaaaga gcggctgccg atgcccacca
     4861 gcttcagagc cgcatcaggc gatgaaatat acgtggggaa gaacaacctc gagaacgatc
     4921 atctcacaac gaagctcgcc cgcccacatg acctgtggtt ccatgcaaaa gacatacatg
     4981 gtgcccatgt agtagtcagg cgaaggccga accaagaagt gccagatgag acgctgcgcg
     5041 ccgccgcgtt gctggcggcc tactacagcc aagcccggat gtcgtcgaac gttgcagtcg
     5101 actatacaga ggctagaaat gttcgcaagc cgcggggcgc acggccggga atggtaatct
     5161 atgaggccca caagaccata tgggtgacgc catcaaggga ggaactctcc aacgccctcg
     5221 gtttcaacgg ggacacttgt cccgggaatg aggattaagc ccctccctcc agtcggcgcc
     5281 acgaccgcgc caagccatgc cgaatccctg ataagcctgt ccagaacagt cctggcggcc
     5341 catcccacca gaggacgggg acggcattcc tctatcctca tgggcagaat ctgcgcagag
     5401 acgattttgc tgggcgaaaa cgtcacgcga gctatcatgc tttccacagt gtcgggcttt
     5461 gtctgatcga acacgaagtt ccctagggag tagaatatga tcccgccgtt gtacacctcc
     5521 acaggctgta gcacgtgggg atgatggccc agcaccagac tggctccggc atcgatcgac
     5581 atgtgggcga tctgcatctg ccttgtgttc ggcatgcgcg catactcctc gccccaatgg
     5641 taggagacga caacatggtc tgccacggcc cgtgccttcc gtatgtcctg gacgatcatc
     5701 ttctcatcca ttggcgcaac cccgggacta tccaggcccg cacgaaaact ccttggataa
     5761 gaataagtcc agaatatatc cgcaagctcc gagtagccca aaaacgcaat cctcaccccg
     5821 cctgcgctga caatcgcagg cgaacgcgcc tcatctaggt cgaacccgcc gccgcacaca
     5881 gctatcgagc tgtccgccaa tgcagcaaga gtctcggcaa aggcggcttc atcgtaatct
     5941 agcacatgat tgttggcgac agtgactacg ttcaccccgg ccttcgcaag ctcctgcgca
     6001 aacttgggat cagacctgaa ccatatgccc ttgcccggga tgggtcttcc gcccacggca
     6061 agactggtct caagattgac aaaccccgca tctgccgaag agaatatggg ggcaacagca
     6121 ctgagcggcc aatccgcccc tcggcgttcg cagacagccc tcactcccct gtcgaatatg
     6181 acatcgccgg cggctgcaag agtgaaccga gattgcagag actcgcaggc cgtacgctgc
     6241 cagacatcct cgagcctaga tccatcgctg cagggatagg ccgcccgacc ctccgaaaag
     6301 cgcaaccacg tacaatcagg agcgcaccct gtacagatca cgcatgaagc cagaacggcc
     6361 aaaagcacct caagcgccgc ggcccgtcga accgccaaat cgacccggcc ggttccgtcc
     6421 gcctttttcg aacccccgtt tcccatcata gaaaccgccc cgctatcttg cgccgcaccg
     6481 cccatcccct gaaggctccc tcgacctaag cagtgcgaga gcggcccgca tgtcatcagg
     6541 catcttcttc gcctcgatcc tgaccctctc cccagccaca ggatgagtaa
//
LOCUS       DAOSAI010000091         6563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7506_length_6563_cov_3.777966, whole genome shotgun sequence.
ACCESSION   DAOSAI010000091 DAOSAI010000000
VERSION     DAOSAI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7506_length_6563_cov_3.777966"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /gene="ftsH"
                     /locus_tag="PLM83_04435"
     CDS             complement(<1..1403)
                     /gene="ftsH"
                     /locus_tag="PLM83_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOK70689.1"
                     /translation="MKSLALWLLLGLIAISISNALFSSKPRVDEITASQFMQYWQEHR
                     VEKLQITGENSIKGTLDDGSGFKLYYPDVKGLVEDLRNDPDLANQVELSMVPEGQGAW
                     WIALIPNIISIILLFVVFFFIMNQVQGGGNKAMQFGKSRAQLQTSERGKVTFQDVAGI
                     DEVVDELEEVVDYLKHPKRYIELGARIPRGILLVGPPGTGKTLLAKAVAGEADVPFFS
                     VSGSDFVEMFVGVGASRVRDMFNEAKRNAPCIIFIDEIDAVGRQRGAGLGGGHDEREQ
                     TLNQLLFEMDGFDPNSGVIVLAATNRPDILDSALLRPGRFDRQIVVDVPDQKGREEIL
                     KVHARNKPLDPEVDLKAVAQRTPGFTGADLANLLNESALLSARNNKKKIGTEEISEAI
                     ERLVAGPAKKGRIMGEREKNLVAYHEAGHAILGHMLPDADPVVKVSIIGRGRAGGYTL
                     MIPEDDRRYRYATKNEM"
     gene            complement(1794..3593)
                     /gene="tilS"
                     /locus_tag="PLM83_04440"
     CDS             complement(1794..3593)
                     /gene="tilS"
                     /locus_tag="PLM83_04440"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOK70690.1"
                     /translation="MGGHPLNVTAKKCIVRHSMIDKGDGIVIGVSGGPDSVALLFFLL
                     DMAKEYDLRLHIAHVNHMIRGADADADADYVEKLAESLRVPVTIERVDVPGVARRSGA
                     TVEDAGRSVRYGLFLRIAKDLGFSKVAVGHNADDQAETVLMRVIRGAGTRGLAGIPPT
                     RDLGEGITLIRPLIGTSRDSIEAYCRAKNVCPRQDATNLDTFYFRNRVRRELLPLLEA
                     NYNSNIRASLTRLAATAADDEAYLRSAADQLLELAIARAGAKVVEQVRYVRPRVEHET
                     EYVTESPIQTAPLQLDLGVLQDAPPALLARVLTAAFAEMADGEHDLYYPHVAAMCDLV
                     AEGSVGDSVDLPRGVRAWLTYDCLLVGHAEDGNLGEEAWDRRQQGQKDRHDWASLKVP
                     GVTSAPACGVIIYSAICEAPQDGAGLACAGHVEADGNAHDDPDGRTSIAMFSRSEWAD
                     SEDGAGKDAGGGFARKDSEPSCAMIGPSALAAIGNMACAASGFEGVDLDPSLLGITWV
                     IFDAGKVDTENLAVRPWKAGDRLAPFGMEGTKKVSDVFIDEKIPKADRLQWPVIVYGD
                     EVFWVVGLRASRIAVVDAKTRRYGVLVAEWDRD"
     gene            complement(3840..>6563)
                     /locus_tag="PLM83_04445"
     CDS             complement(3840..>6563)
                     /locus_tag="PLM83_04445"
                     /inference="COORDINATES: protein motif:HMM:NF039380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOK70691.1"
                     /translation="GSATLFGAYAPFGIVCVTAALFGWDVAYAMPTIAGALAGAALGA
                     GPQAAVGLAISAALLEVLRKLIWPQLSPGFRHSTGSWAVGAIVLLCSFIGSAIAYEVS
                     GSGVDVLVVSLVESALAGSAAAIVVPSAIACAETGQLKSAGRLESAALLVVGAAVCAG
                     LDSWSFGGFSPGAAVGLYASAVAGAIAGPGAGASVGASLGVGLGIARAGQGILIGILT
                     LAGAASGIVGRQGRAVTAFSILIAAALSGYQLRTTVEFTARMVEASVAAIGFLLTPDR
                     LLSELSWRLPGGIAPYNLERERMDGVYHVVMGKLSVLGQTFRELAIAYQGGPIGDFRD
                     TDGGERKDGSDADGHDGAGSAADIDLAGGLGPAEIWSSDVQELDDPFEAASCESPGMR
                     AVRAAAFIRTQACTGCPRYDVCWRDMFFRTYRDIVDMLAAVELFGDGGIESLPEGLKN
                     RCMRTEKVIGASLVAAGRRRSAAVWQGQKERNGQQKTEALLDRSSTVSILALQMAGVA
                     DAISRVSCQAQGMARFDEEAERLIRRRLSAMGIGVVDVRVSWTGHGGLEVYVVKAPCA
                     QDGECVKTVAPAVGDALGRPLSVWQADCFWTEGEGSMTLGPEGKSRVGAATGLESVRR
                     DRRGERKECCIRLLPARKYDLESEAVTLPRTPGETSGDSHAVINLGDGRMAAIIADGM
                     GVGEEASAESGAAVAMLARLLAAGLDLDFAVQSVNAMMLLRSEDDTFTTVDLAVVDLY
                     TGQAEMVKAGAPPSFIKRGHEVIAVGGPSVPVGVLAPTRVMRYRTNLEEGDMLIMVTD
                     GVTEARPGPSESGVELVRVMERADQDWPRKVVADVIDWARGADFMDEDSSGQSRGGTR
                     QATAKHGNRAEARRGNPMGPTGDKKRSGSAQSRDDMTVLAVRLTLAKGAQT"
BASE COUNT         1457 a         2173 c         1539 g         1394 t
ORIGIN      
        1 aacatctcat tcttggtggc gtaacggtac cttcgatcat cctcaggaat cataagtgtg
       61 tagccgcccg ctctgccgcg tcctatgatg gacactttca ccacggggtc agcatctggg
      121 agcatgtggc caagaatcgc gtggcccgcc tcgtggtacg ctaccaggtt cttctccctc
      181 tcccccatta tccgaccctt tttggcggga cccgccacca gtctctcaat agcctcggaa
      241 atctcttcgg tcccgatttt cttcttgttg ttccttgcgg aaagaagagc actctcgttc
      301 aagagatttg ccaggtccgc cccggtaaac ccgggtgtgc gctgcgctac tgccttcagg
      361 tcaacttccg gatcaagagg cttattgcgc gcatgcacct tgagaatctc ctcccggcct
      421 ttctggtcag gcacgtcaac tacgatttgc ctgtcaaatc tacccggcct cagcagcgca
      481 gagtcgagaa tgtcgggcct gttagtcgcg gccaacacga tcactccgga attcggatca
      541 aacccgtcca tctcgaaaag caactgattg agagtctgct cgcgttcatc atggccgccc
      601 ccaagaccag cgcctctctg acggcccacc gcatcgattt cgtctatgaa gatgatgcat
      661 ggcgcgttgc gttttgcctc attaaacatg tcacgaactc ggctggcgcc gacacccacg
      721 aacatctcca caaagtcgga accgctcaca ctgaagaaag gaacgtccgc ctctcctgca
      781 accgccttag ccaagagcgt cttgcctgtt ccaggaggtc caaccaagag tatcccccgg
      841 ggtatcctgg cgcccagctc aatgtagcgt ttagggtgct tgaggtagtc cactacctcc
      901 tcaagctcgt ccacgacttc gtctattccg gcgacatcct ggaaagtcac ctttcccctc
      961 tcggacgtct gcaactgagc ccggctcttg ccgaactgca tcgccttgtt tcctccacct
     1021 tgaacctgat tcatgatgaa gaaaaagaca acaaacagaa gaattatcga tattatgttg
     1081 ggaataaggg cgatccacca tgccccttgt ccttcgggca ccatagagag ctccacctga
     1141 ttggcgagat ccggatcatt acgcaagtct tctactagtc ccttcacgtc ggggtagtag
     1201 agtttgaagc cagatccgtc atcgagtgta cccttgatag aattttcacc cgttatctga
     1261 agcttctcaa cccgatgttc ttgccagtat tgcatgaact ggcttgctgt aatctcgtcg
     1321 acgcggggct tgcttgagaa tagcgcgttc gatattgata tcgcaatgag gccaagcaac
     1381 aaccacagcg cgagactttt cattagcctg ttattcacct tgttaggcgc aacaagcccc
     1441 agccttcaaa gatggcgagg aatgcgcctt ccctccttta atagaatatc ccgcgcagta
     1501 cgccacgggg aaatattcgg tttcaaattt cgtaatatgc gaagtaatta tagcacagcc
     1561 ctcctgcgcg acacaaacaa ggcccaaggc ccaagacaaa atgattccac agacaaccaa
     1621 aagacccgta tgacctatgt gtatcgttgt actttatcct taacgaccat gagcccttca
     1681 atgttcctac ggaatgcaga atgtccgtcc tgaagcactc agcactctca gatgcggtcc
     1741 cttcggcact ccagacagag gcggctgcgg agccctgtat tggcgccgga ctctcaatcc
     1801 cgatcccatt cggccacaag caccccgtac ctcctggttt tcgcatctac cacagcaatt
     1861 ctgctcgccc gcagacccac aacccaaaaa acctcgtccc catagactat cacaggccac
     1921 tgcagcctgt cagccttggg aatcttttcg tctataaata catcagaaac ttttttcgtc
     1981 ccttccatgc caaacggggc cagcctatcc cctgctttcc aggggcggac cgctaggttc
     2041 tccgtatcga ctttgcctgc atcgaaaatc acccaggtga ttccgagaag cgatggatca
     2101 agatccacgc cctcaaatcc acttgcagca caagccatgt ttccaattgc tgcaagagct
     2161 gaaggtccta tcattgcgca gctgggctcg gaatccttcc gggcaaaacc gccgcctgca
     2221 tctttccccg cgccgtcctc agaatcggcc cactcggatc tcgagaacat agcgatggag
     2281 gttcgtccat cagggtcatc atgggcgttg ccgtcggcct ccacatgccc tgcacacgcc
     2341 aaacctgctc catcctgagg cgcttcgcag atagccgagt agatgatcac cccgcaagca
     2401 ggcgcggagg ttacaccagg aaccttcaga cttgcccagt catgcctatc cttctgccct
     2461 tgttggcgcc tatcccaagc ctcttcgccc aggttcccgt cttccgcatg cccaactagt
     2521 aggcaatcat atgtcaacca tgctcgtacg cccctgggca agtctacgga gtcgcctaca
     2581 gatccctcag ctaccaaatc acacatcgcg gccacgtgcg gatagtatag atcatgttct
     2641 ccatctgcca tctccgcaaa cgcggcagtc aacaccctag caaggagcgc aggcggtgca
     2701 tcttgcagca ctccgaggtc gagctgtagc ggcgcggttt ggatggggga ttcagtcaca
     2761 tactctgttt catgctcgac tcgaggcctc acgtaccgaa cctgctccac aaccttggcc
     2821 cccgcccgag caatcgcaag ctccagcaac tgatcggcgg cagacctcaa ataggcctca
     2881 tcatcggcag ccgtcgcggc aagcctggtc agggatgctc tgatattcga gttgtagtta
     2941 gcctcgagta acggaagcag ctcccgacgc acacgatttc taaaatagaa agtgtccaag
     3001 ttcgtcgcat cctgccttgg acagacattc ttcgctctgc agtaggcctc tattgaatcc
     3061 ctacttgtgc ctataagcgg cctgatcaac gtgattcctt cccccagatc gcgggttggc
     3121 ggaatcccgg caaggccacg agttccagcg ccacggatca ccctcataag cacggtctct
     3181 gcctgatcat ccgcattgtg tccaacggcc actttgctga atcccaaatc cttggcgatc
     3241 ctcaaaaaca ggccatacct tacggacctg cctgcatctt caaccgttgc cccgcttctg
     3301 cgggcaaccc caggcacatc tactctctct atagtcactg gcactcttag gctctcggcg
     3361 agcttctcaa cataatccgc gtctgcatcg gcatctgccc cacggatcat gtgattgacg
     3421 tgggcaatgt ggagcctcag gtcgtactct tttgccatgt cgagcaggaa aaaaagtagg
     3481 gccacggaat caggcccacc cgagaccccg atcacgatcc cgtctccctt gtctatcatc
     3541 gaatggcgca caatgcactt cttggctgtg acattcaatg gatgtccgcc catttagcgc
     3601 tgcctcccac atttcacgtc agattcagta tcaagcgtca ttacataata ggcaatcccc
     3661 aactgtaatc ctgcagcaac tgacaatctg agctgcacag aacaatgccg aaggagcaag
     3721 atagccccct ccggtttgtg gtgtttttgg ccatcggatt tccgcagttg gcaaaggcgc
     3781 ccgccaacgg aaccaataga acccttattc atatgatatc acaaaaaaga cgccccacat
     3841 catgtctgag cgcctttggc cagagtaagc ctgactgcaa gcacagtcat gtcgtctctg
     3901 ctctgggcgg agccgctccg cttcttgtcg cccgtgggcc ccatgggatt gcctcggcgt
     3961 gcttctgcgc gattgccgtg tttcgccgtt gcctgccgag tccctccgcg gctttgtccg
     4021 gaagaatcct cgtccataaa atccgcccct ctcgcccagt caatcacgtc cgcaacaacc
     4081 ttcctcggcc aatcctgatc tgcacgctcc atcacacgga caagctcgac acctgattcg
     4141 gacgggcctg gccttgcctc ggtcaccccg tcggtgacca ttatgagcat gtccccttcc
     4201 tcaagattag tcctgtacct catgaccctc gtgggcgcca aaaccccaac cggcacagag
     4261 gggcctccta cggctatcac ctcgtgtccg cgttttatga agctaggcgg ggcgcccgct
     4321 ttgaccatct ctgcctgccc ggtgtaaagg tccaccacgg caaggtcaac agtggtgaat
     4381 gtgtcatcct ccgacctgag caacatcatc gcatttacac tttggaccgc gaaatccaga
     4441 tcaaggccgg ctgccaagag cctcgccagc attgccacgg ctgcgccgct ctctgctgaa
     4501 gcctcttccc caacccccat gccatccgct attatggctg ccatccttcc atctcccaag
     4561 ttgatcactg cgtgggaatc gccgcttgtc tcgcccggag ttctgggcaa tgttacagct
     4621 tcgctctcaa gatcatactt tcgggcaggc aacagcctga tacagcactc cttgcgctct
     4681 cctcgccggt ccctacgaac gctttccaac cctgtcgccg ctccaacacg gctcttgcct
     4741 tcaggcccaa gcgtcatcga accttctcct tcagtccaaa agcaatccgc ctgccaaacg
     4801 gagagcggcc ggcccaacgc atcgccgact gcaggcgcca ctgttttgac gcactctccg
     4861 tcctgcgcgc acggagcctt gaccacgtac acctccaaac ccccatgacc ggtccaggat
     4921 accctgacat ccacaacgcc aatgcccatg gccgacagcc gccttcggat caacctttca
     4981 gcttcctcgt caaacctagc catcccctgc gcttgacaag atacgcgcga gatcgcgtcg
     5041 gccacaccgg ccatctgtaa tgccaagatg ctcacagtcg atgacctgtc gagaagcgct
     5101 tccgtcttct gctgcccatt tcgctccttt tgtccctgcc atacggcagc gctccttctt
     5161 cttcccgcag caaccaagga ggctccaatc actttttccg tccgcatgca tctatttttg
     5221 agcccttccg gaagcgattc aatgccacca tccccgaata actccacagc tgcaagcatg
     5281 tccacaatgt cccggtaagt tcggaagaac atgtcgcgcc aacacacatc gtatcttggg
     5341 caaccggtac atgcctgtgt ccgaatgaag gctgccgcac gtactgcgcg catcccgggg
     5401 ctctcgcagc tcgcggcttc gaacggatca tccagttcct gaacgtcaga tgaccagatc
     5461 tctgcaggtc ccaaaccgcc cgcaagatcg atatcggcag cagaaccagc cccgtcatgc
     5521 ccatcggcgt cgcttccatc cttcctttca ccgccatctg tgtcgcgaaa atccccgata
     5581 ggcccgcctt gatatgctat tgcaagttca cgaaaggtct gccccaaaac ggaaagcttc
     5641 cccataacca cgtgatagac accgtccatc cgctcccttt caagattata aggggcaata
     5701 ccgccaggaa ggcgccacga aagctctgaa agaagccgat caggggtaag aagaaacccg
     5761 atcgcagcaa ctgacgcttc aaccattctg gctgtgaact cgactgtcgt tcgaagctgg
     5821 taacctgaga gcgcggctgc gatcaatata gaaaacgccg tgactgcccg tccctgcctg
     5881 ccaactatgc ccgaggcggc cccggccaat gtcaaaatgc caatgaggat accctgtccg
     5941 gctctggcga tgccgagtcc gacgcccaaa gatgctccca cgctcgcacc tgcgcccgga
     6001 cccgctatcg cacctgcaac cgccgacgca tagaggccaa cggctgcgcc gggggaaaac
     6061 ccgccaaaag accatgaatc aaggcctgcg cagaccgcgg cccccacaac aagaagcgca
     6121 gccgactcaa gtcggcccgc cgatttgagc tggcccgtct cggcacaggc gattgccgaa
     6181 ggaaccacga tggccgctgc cgacccggcc aaggcggact cgacaaggct gacgactaac
     6241 acgtcgacgc ctgatccgct tacctcgtag gctatcgccg atccaatgaa cgaacacaag
     6301 aggacgatcg cgcccacggc ccagctacct gtggaatggc ggaaaccagg cgacagctga
     6361 ggccagatga gcttccggag aacctctagc aacgcagcgg atatggcgag gccgaccgcg
     6421 gcctgcgggc ccgccccaag ggccgctccg gctagggcgc ccgcaattgt gggcatcgca
     6481 tatgcaacat cccatccaaa aagcgctgcg gtcacacaaa caatgccaaa cggggcatag
     6541 gcaccaaaca gggtagcaga ccc
//
LOCUS       DAOSAI010000092         6514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7568_length_6514_cov_3.217990, whole genome shotgun sequence.
ACCESSION   DAOSAI010000092 DAOSAI010000000
VERSION     DAOSAI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7568_length_6514_cov_3.217990"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2660
                     /locus_tag="PLM83_04450"
     CDS             <1..2660
                     /locus_tag="PLM83_04450"
                     /inference="COORDINATES: protein motif:HMM:NF025681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOK70692.1"
                     /translation="EIIAVERRPVRDESNRTKIVYTITAHDVASGSLYVRFEDSNTED
                     GWGASFRGGPLVVYSSADVADGYSYFPVLERPERLYVIRESAMDAAEFLTIISIQGIV
                     AQDRPEIYIQGAGNYGAWVDMFERDYGIPVEYHNDPMWFIEHYRNLFETNGYILTALE
                     TEAVNVATTAAGVLRAPIVDESLKPLMDSLGFEMVLDTTDLDEAWAFDTYKEYVTEDL
                     VVRQEWGKIHLRDYAIATKAFTFYDLEDIFFMKDIYDWVRRDIPLIGWGPGSEGSHIG
                     VATMMGLFTVPADWALNLTVFPAVSVAKLSQPNHVANIRFETDVHYVTFVMSDGDNVQ
                     WFLNDMPTSKRHYGSDRRGEFPMGFTISPSLADLAPTVMKWYYDTAKLDYYVAGISGT
                     GYMYPVSFPELDSYVDKCNEYLERSDLSIVSLHDPTPMNTPEFLEMAEAYARQPNVLG
                     GIYLTGRRYAGEEGNVVWADGKPFVGVRESLWTDDPVEMAGRINSYAADPSVPEGYTI
                     VNVHPWSHSLDDVATVIDNLAPHVRVVNPEEFFLQMIYNLGGRFPTVRTDVEAKISSP
                     VSREKVSGIIDVCGNASSSSAIAMVLATIDDMPWIIAEGADEWNVSLDTRKVADGPHA
                     LRVIAFDEDGKNCQVSEEIMVMNQSDSGTVVGWVMYAHSPVAGAAVYLGERTCVTDQD
                     GRFEFKFVPSGMATLKVQAEGFTGISVDVAVAANQASVVELAFPVDPDTRRVWTFDED
                     ADGWKGGYISPGAPHMGKAKWDKNIGDGPGSLCMDGSDYGYPDGKANAWFYNIVKLPV
                     DAENISYKTRGNEKAGGIGGGLRLVLVDEEGEIHELTPWEASNAAEWVVRTADISEFA
                     GRTVVLRFEQDDLDVGISEHRWVDNVIIR"
     gene            2704..4209
                     /locus_tag="PLM83_04455"
     CDS             2704..4209
                     /locus_tag="PLM83_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70693.1"
                     /translation="MLVYRGTLTVAAIHDKRQGTMVKMAFWTTGDFHTHTFLTDGSHT
                     LFEVARRAFEIYGLDWIANSEHGGSSSRGPAGMYWENSLGPEEFEGILIKDEAGRRAM
                     WRWQSLRDYSFPLVERLRDTYPNNVIIQGVEWNMPCYDHVSVGIAADEPVAISDFEYM
                     FDQNDEDASRTHLNQFKRNSTREDAIAGVRWLNEHYRDSSYAVINHPSRAMKYAPSDL
                     RDLNNAGPDVCFGFEGLPGRQKKSFRCGYSQDFAGMTPLCRTYGGADPFLATIGGVWD
                     SLLGEGRRFWVFANSDFHEAGSDSDFYPGEYAKNYVKVDENSPKGIVDGLRSGNCFVV
                     HGDLIDHLDFWAECGSSAATMGESIWVPAGSDVVVTVRLRSPHEDGVDHVDLIAGRVF
                     GLIVPEDARYNVGNNLSTHVVARFFTKGKLLDDKGFASMSHVIRNVRSNMYVRLRGTN
                     LPVGAALETDFDGDPLSDALAHLGGYDNEMEAYHDLWFYSNPINIRVEGAS"
     gene            4351..5271
                     /locus_tag="PLM83_04460"
     CDS             4351..5271
                     /locus_tag="PLM83_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK70694.1"
                     /translation="MGSVDSTVPSCVGLYRTGELARRAEEAVRRLSRCVICAQACNVN
                     RIEGELGFCRTGRLAVVSSAGRHFGEEDVLVGTGGSGTIFFANCNLACVFCQNYDISA
                     CGFGKEVSRRRLADMMLSLQAKGCHNINLVSPSHVVPQVLEALVLAVEEGLRLPLVYN
                     TGGYDALSTLKLLDGIVDIYMPDFKFADADDAERFSAAPRYPTVAQAAIREMNRQVGD
                     LVVDEDGVARRGLILRHLVMPGKLDDTREIMRFVVRDISRDTYVNVMGQYRPAHLASR
                     YPELSRALGISEHQRAIEIAREEGLYRFAR"
     gene            complement(5410..6021)
                     /locus_tag="PLM83_04465"
     CDS             complement(5410..6021)
                     /locus_tag="PLM83_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70695.1"
                     /translation="MQIPWKSIVIVIFLVLAGLGFYASGPLARADTVTQVIRESVWIE
                     APNRFEIQGKNECTGFAVAYVLRHFGIEVTGKEIYDAVRFKLPSGRVLPRGPINELRR
                     HGIQAQVYTGTLNTLKARLSNGLKWMHYFVLVGYDEPTKELHFYDPEIAEYGNGKAPG
                     NRILTEEQFLKLWNTHIPGFRNIYIVCTPSEGRHNSGLNAFKR"
BASE COUNT         1546 a         1484 c         1888 g         1596 t
ORIGIN      
        1 acgagatcat tgccgtagag cgcagacctg tgcgggatga gtcgaacaga actaagatag
       61 tgtacacgat tacagcacat gacgtggcct ctggatcgct atacgttagg ttcgaagaca
      121 gcaacacgga agacggctgg ggtgccagct ttcgaggcgg accgctcgta gtatacagtt
      181 cagcggatgt agctgacggg tattcatatt tcccagtatt ggagaggcct gagcgtcttt
      241 atgtaataag agaatctgcc atggacgccg ctgaattcct gacgatcata tctatccagg
      301 gcatagttgc gcaggacagg ccagaaatct atatacaagg ggcaggcaac tacggtgcat
      361 gggtcgatat gtttgaaagg gattatggaa ttcctgtgga gtaccataac gacccaatgt
      421 ggttcattga gcattacagg aacctatttg agacgaacgg ctacatccta actgctttgg
      481 agactgaagc ggtgaacgtg gcaacgaccg cagctggagt actcagagct ccaatcgtgg
      541 atgagtcgct caaacccctc atggattctt tggggttcga gatggtccta gatacaactg
      601 acttggatga ggcatgggcc tttgacacat acaaggaata tgtaactgag gaccttgtcg
      661 tgaggcagga atgggggaag attcacctca gggattatgc catagctacg aaggctttca
      721 ccttctacga ccttgaagat atattcttca tgaaggacat ctatgattgg gttcgtcgcg
      781 atataccgct gataggatgg ggtcctggaa gcgagggttc gcatataggc gtagctacta
      841 tgatgggcct gtttacagtg cctgccgatt gggctttgaa cctgactgta tttcctgcgg
      901 tttcagtggc gaagctgtct cagcccaacc acgtggccaa catcagattt gaaaccgacg
      961 tgcactatgt cacattcgtc atgagtgatg gagacaatgt gcaatggttc ctcaacgaca
     1021 tgccaacgag caagaggcat tatgggagcg acaggcgagg ggaatttcct atggggttca
     1081 ccatttcgcc gagcctagcc gatctggcgc ctacagtaat gaagtggtac tacgacaccg
     1141 ccaaacttga ttactatgta gccggcattt cggggactgg ttacatgtat ccagtcagtt
     1201 ttcctgaact cgattcatat gtcgacaaat gcaatgagta cctggaacga tccgatctgt
     1261 ccatcgtgag cttgcatgat ccgactccaa tgaatactcc ggaattcctg gagatggcag
     1321 aggcttacgc gaggcagcct aacgttctag gcggcattta cctgactggt cgacggtatg
     1381 caggcgaaga agggaatgtg gtgtgggctg atggcaagcc attcgtgggg gttcgcgaat
     1441 ctttgtggac tgatgatccg gttgagatgg caggcaggat aaactcttac gcagcagacc
     1501 cctcagttcc tgagggctat actattgtga atgttcatcc gtggagccac agcttggatg
     1561 atgtggccac ggtaatcgac aatttggcgc cgcatgttcg cgtagtcaat cccgaggagt
     1621 tctttctgca gatgatatac aacctgggag ggagattccc taccgtaagg acggatgtgg
     1681 aggccaagat atcctctcct gtaagcagag aaaaggtatc gggaataatc gatgtttgcg
     1741 ggaacgcatc tagttcgtct gcgatcgcga tggtattggc cacaattgac gacatgcctt
     1801 ggattattgc tgaaggcgcc gacgagtgga atgtatcttt ggatacgagg aaggttgctg
     1861 acggacctca tgcgctgaga gttattgctt ttgatgagga cggcaagaac tgccaagtat
     1921 ctgaagagat catggtcatg aaccagtctg acagcgggac tgtcgttggt tgggtaatgt
     1981 acgcgcactc gcctgtggca ggtgccgcag tctacttggg agagaggaca tgtgttaccg
     2041 accaagacgg caggtttgag ttcaagttcg tccctagcgg gatggcaacc ttaaaggtgc
     2101 aggcggaggg attcaccggc atcagcgttg atgttgctgt agcggccaac caagccagcg
     2161 tagtggagct cgcgttcccg gtcgaccccg atacgcgtag ggtctggact ttcgacgaag
     2221 atgctgatgg ttggaagggc gggtacatta gtccgggggc gccgcatatg ggaaaggcca
     2281 agtgggataa gaacataggc gatgggccgg gaagcctttg catggacggc tcggattacg
     2341 gataccccga cggcaaggcg aatgcctggt tctacaacat agtgaagctt cctgtcgatg
     2401 ctgagaacat cagctataag acccgtggaa acgaaaaggc cggaggaatc ggcggcggac
     2461 tacgtcttgt gctcgtcgac gaggaaggcg agatacacga gctgacgccg tgggaagcga
     2521 gcaatgccgc agaatgggtt gtcagaacgg cagacatatc cgagtttgca ggtcgcacag
     2581 ttgtgctcag atttgaacaa gacgatttgg atgtgggaat atcggagcat cgctgggtcg
     2641 acaatgtgat catcagataa gacaacgaac tttacttgtg aatgtgccgc ggtggcgcag
     2701 gcaatgcttg tctaccgcgg cacattgact gtggctgcaa ttcacgacaa acgacagggg
     2761 acgatggtga aaatggcatt ttggacaaca ggtgatttcc acactcacac atttctcacc
     2821 gatgggagcc atacgctttt cgaagtggct cgtcgtgcat ttgagatata cgggctagac
     2881 tggattgcca attcagagca tgggggatca agttcacgtg gccccgcagg catgtactgg
     2941 gagaacagcc tgggtcctga ggaatttgag ggaattctca taaaggacga ggcaggacgt
     3001 cgtgctatgt ggagatggca atctctgcgc gactactcct ttcctttggt ggaaaggttg
     3061 cgggatacgt atccgaataa cgtgatcatt cagggcgttg aatggaatat gccatgttat
     3121 gaccatgtaa gtgttggaat tgcggctgat gaacctgtcg ccatatccga ttttgaatac
     3181 atgttcgatc agaatgatga agatgccagt aggacacatc tcaaccaatt caagcgcaac
     3241 agcacgcgtg aggatgccat agctggagtt cgctggctca atgaacacta tcgagactcc
     3301 agctatgcgg tgataaacca tccttcgcgc gctatgaagt atgcaccgtc cgatctgagg
     3361 gatctgaaca atgcaggccc tgacgtctgt ttcggctttg aaggcctgcc gggaagacag
     3421 aagaagagct tcaggtgcgg ctatagccag gattttgccg gtatgacacc actatgtcgg
     3481 acatatggcg gagccgatcc cttccttgct acaattggag gggtgtggga tagcttgctg
     3541 ggagaaggtc gcagattttg ggtttttgct aattccgatt ttcatgaggc tgggtccgat
     3601 tctgatttct acccgggcga atacgcaaag aactacgtga aggttgacga gaacagccca
     3661 aagggaatag tggacggatt gaggtccggg aactgttttg tcgtccatgg agatcttatt
     3721 gaccacctcg atttctgggc cgagtgtggc agcagcgccg caactatggg cgagtcaata
     3781 tgggttccgg ctgggtcaga tgtggttgtt actgtgcgtt taagaagtcc ccatgaagat
     3841 ggggtggatc atgtggactt gatcgccgga agagtattcg gcctgattgt gcccgaagat
     3901 gcgagataca acgtgggaaa caacctatct acgcacgtgg ttgcaagatt cttcacaaaa
     3961 ggcaagcttc ttgatgataa gggttttgcc agcatgagcc atgtgatccg gaatgtgcgg
     4021 agtaacatgt atgtgaggct ccgaggtacg aatcttccgg taggggcggc gctggagaca
     4081 gacttcgacg gcgatccgct atctgatgct ctcgcccacc taggcggcta tgacaatgaa
     4141 atggaagcct atcacgatct gtggttttat tcgaacccga tcaacataag ggttgagggt
     4201 gctagctaag tgctatgcgc gggagctgca tactgagagt aagaccaaat ctcatgtccc
     4261 cttcctgttt tgcatctgtt atgatgtggt atgataatcc gcaggtagct gcataggagc
     4321 gttattgctc caaaacaaag gagtcttttg atgggttcag ttgactctac agttccaagc
     4381 tgtgttggcc tttaccgcac aggcgagctg gcgaggcgtg ctgaggaagc ggtaagacgg
     4441 ctttcccgtt gcgtgatatg tgcccaagcc tgcaacgtca accgtataga aggcgagctg
     4501 gggttctgcc gaacgggaag gctagctgtg gtgtcaagtg ccggccggca cttcggtgag
     4561 gaggacgttt tggtgggaac cggcggctcg gggacgatct tctttgccaa ctgtaacctt
     4621 gcgtgcgtct tctgtcagaa ctacgatatt agcgcgtgcg gtttcggaaa agaggtctcc
     4681 cgtcgtagat tggccgacat gatgttaagc ttgcaggcca agggctgcca taacatcaat
     4741 ttagtctccc cgagccatgt cgtgccccag gttttggagg cgttggtcct cgcggtagaa
     4801 gaagggctga ggctgccgct tgtgtacaac acagggggtt atgatgcact ttcaactttg
     4861 aaactgcttg atgggatagt tgacatctac atgcctgact tcaagttcgc agatgccgat
     4921 gatgcagagc ggttttccgc cgcaccgcga tacccgactg tggcgcaggc tgcgataagg
     4981 gagatgaatc ggcaggtagg tgacttggta gtcgatgaag atggggttgc ccggcgtggg
     5041 cttattctgc ggcatttggt gatgccgggg aagttggacg atacacgtga gataatgaga
     5101 tttgtggtcc gggatatctc gcgcgacact tatgtgaatg taatgggcca gtatcgacct
     5161 gcccaccttg cttcccggta tcccgagctt agcagggcgc ttgggatatc cgaacaccag
     5221 agggccattg agatcgcgcg cgaggagggg ctgtatcgat ttgcgaggta gacttcactg
     5281 gcttcgtgaa gtcgaagctg agtccgtatg ccataagccg ttgcgcaggt gccattgatg
     5341 cacgtctagg tggttcctga tctgtctttg aattgttggc aagcatcagc ttgcttccaa
     5401 atctaggcat tatcgtttga aagcgttcag gccgctattg tgcctgcctt ctgatggtgt
     5461 acatactatg tagatatttc ggaagcccgg aatgtgagtg ttccacaatt tcaagaactg
     5521 ttcttctgtc aatatgcgat ttcctggggc tttgccgttc ccgtattcag ctatctcagg
     5581 gtcataaaaa tgcaattcct tggtgggctc atcgtatcca acaagcacaa agtagtgcat
     5641 ccacttcaat ccatttgaga gcctggcctt gagggtattg agtgtcccgg tatagacttg
     5701 agcttggatg ccgtgtcgac gaagctcatt aatgggtccc ctcggcaaga ccctgccaga
     5761 cgggagcttg aacctgacag cgtcgtagat ttccttgccg gttacctcga ttccgaagtg
     5821 tcggagcaca tacgcgactg caaaccctgt gcactcgttc ttgccctgaa tttcaaatcg
     5881 gttgggcgct tcgatccaga cggattcacg gataacctgc gtcacagtat cagctcgtgc
     5941 taggggaccg cttgcataaa acccaagacc agcaaggaca aggaatatga cgatgacgat
     6001 gctcttccac ggaatttgca aatcgaatgc acctccccat ctttgcccat ctcagtctct
     6061 gcatatagat tttgcgatgc tccttgaggg ttctttcagg ccccagttgc ccaatgaaag
     6121 ccgggacact tcttgtgact tgccaagaag aagactattc gctgcctacc ctatgccatt
     6181 ttttagacgt gagctcaatc cgggtcaaca ggttaggctc cgggcatgcg cccctaaatc
     6241 cctgcccaaa acgatcatta tgcagccatg tatattccat ggtgtggctt aagatggcat
     6301 gctatagcag atcatgatcg ggcacatgtc gaaatggagc ccgaacccgt tgccatgtcg
     6361 aaaaaccgcc acaatgtcac ttccaaagcc agcggggcta tcagctctct cgccggttac
     6421 agatcgggtt ggcttcaata tatattacct tttcatgcgg gatgtggagt atgcgattcg
     6481 tgcctgactt aacatgacat ctagttgctc tcat
//
LOCUS       DAOSAI010000093         6455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7646_length_6455_cov_3.668281, whole genome shotgun sequence.
ACCESSION   DAOSAI010000093 DAOSAI010000000
VERSION     DAOSAI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7646_length_6455_cov_3.668281"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLM83_04470"
     CDS             complement(<1..402)
                     /locus_tag="PLM83_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70696.1"
                     /translation="MDMSDLAALVVWIVVIFSYVLRIVARGFKQAEEEALKKARHLEA
                     SRDAQAEGRMEPSAPADGFAGSPEVPSTLQSYELESVEAEEDYDAWPLPKTRRSLAQE
                     AAEERAYGREVEMAEPIVTGSLGEAGDDASEW"
     gene            complement(503..1504)
                     /gene="floA"
                     /locus_tag="PLM83_04475"
     CDS             complement(503..1504)
                     /gene="floA"
                     /locus_tag="PLM83_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496556.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOK70697.1"
                     /translation="MAIGWIILIVVLVLLLVLLNFIPVGLWISAWAAGVNVGLLTLVG
                     MRLRRVPPVRIVLPMVKAAKGGIPVTVDKLEAHYLAGGNVDRVVDALIAAQRADINLT
                     FERAAAIDLAGRNVLEAVQMSVNPKVIETPVIAAVAKDGIELKAKARVTVRANIDRLV
                     GGAGEATIIARVGEGIVTTVGSAESHKEVLENPDQISRTVLNKGLDDGTAFEILSIDI
                     ADVDVGRNIGARLQMDQAEADKNIAQAKAAERRFAALALEQENKAKVQEMRAMVVEAE
                     AEVPRALADALRSGNLGVMDYYNLLNLKADTQMRDSISKATGAPGSSEGSGSDAGLK"
     gene            complement(1509..2840)
                     /locus_tag="PLM83_04480"
     CDS             complement(1509..2840)
                     /locus_tag="PLM83_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOK70698.1"
                     /translation="MRAKRGVIGVFVLALIVVGLLVPAGKCAAAPKVYIVPLQGQIEH
                     GLASFIARGLREASDAGADVVIIDIDTPGGYVDSALKMRDAIISSEVRTIAYVDSRAW
                     SAGALIAIACDDIAMKMGSSMGAAETRPKEEKYISAVRAEFEATAELRGRDPAVAGAM
                     VDADVEIPGVIERGKILTLTATAAENLGFIEYKVKDLDDLLGLLGLSGAEIVDVKQTS
                     AEWFARLLTNQVAAEVLLTLGLVGLAVELVTPGFGIPGTIGIVSIGLFFGGRIMAGLA
                     GWEVIVLFLVGLTLLLLEAFVIPGFGVAGILGIVSVIASFVLSYPSPGEGAAALGIAI
                     GIAIAVVALVIFLLSRGGRKGRAPAKLVLDHTETPEEGYLAVETYESLYGAEGMVFTP
                     LKPVGIVDFDGRRVEAISEGPFVMGGARVRVIRIEGNKVFVRPITQMDQED"
     gene            complement(2965..3426)
                     /locus_tag="PLM83_04485"
     CDS             complement(2965..3426)
                     /locus_tag="PLM83_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOK70699.1"
                     /translation="MSIAVRLRDDLKIAMKSKDELKKSVIRLALAGLKNAELEKGAPL
                     SESEEVDVMARQAKMRRESIEEYEKAGRFDAADVERKEMEIIMSYLPRQLSEDELRQI
                     ISETIAQTGAEEPRDIGKVMGAVMPKLRGRADGKLVNSMVREMLDAQGGEE"
     gene            complement(3522..4862)
                     /locus_tag="PLM83_04490"
     CDS             complement(3522..4862)
                     /locus_tag="PLM83_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOK70700.1"
                     /translation="MTADNMDYVDVSSDHGSHPLGRRLTVAACVLCVVILVFALGGCW
                     DARDIDHIGFIMGMGVDESDDGKIVVWVQMALPSSGPEGAANTPAWLGSAAGNTMMDA
                     MSALRMKSAKTLFRGHVKVLVIGEKIARKGLTPILDYLARDSQFRYNSWVLVTSDPVG
                     KIFEVEADQENMASSYINLMMRHATLSSVAPQSRFRDLLSTAAEPGDQPVLARVSLVK
                     SEPEEEEGEQDEAQQGVWGQRTGNEGGKGKKGDVETKSDIEISGSAALHGDLVVGWLD
                     PVQTAMVLMARNRLKEYSFLHMVPGTEGDTVGVKVLHSRATIELPPRHKMTRTGLRGA
                     EVRIRVTGCVYIEEVMSEEQFVSIEADRNLSRGVSDQIEAEMESLIEIAQNRLGTDIF
                     GIGEAVRRRISSDVWEEEIAPIWRDIFKEIKIIPEVSLKVERQGTTMTSPRPQA"
     gene            complement(4849..5937)
                     /locus_tag="PLM83_04495"
     CDS             complement(4849..5937)
                     /locus_tag="PLM83_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HOK70701.1"
                     /translation="MYYTQVVVLVAVIVFSTGFLAAARLLTPVAGTAAWISVLLGGVI
                     GLVIAWLVGSVSSAFPEQNLVKIAEDIFGKPFGKVIGLTFALYCTVVVVLGTREFVLA
                     ISISFLERTPFYVTTAVFFILASYAAYLGIEAIARVCFIFFGVVIISIAILGVFAIPV
                     LVPGRLLPLAGRGVIPIMRAALLTASYTGEAIVSLAFLRHLKDKRHVSRAVGTGVAIG
                     TAIMAMTVAYGIMMLGTGGISRVTYPAVETARMVGAGEFLERGEILFLALWFSVAILK
                     LASVFYAAITAIADLLNLDNYRRLILPFGIISMVLSSIPANVAQSFAAVEYFLKYTVW
                     YAIALPALLLIGARVLRHEKGGTPDDSR"
     gene            complement(6073..>6455)
                     /locus_tag="PLM83_04500"
     CDS             complement(6073..>6455)
                     /locus_tag="PLM83_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOK70702.1"
                     /translation="PHYPTGLAIRLLRFPLLFLSASLGLFGLIAGLIAIALHLGGLTS
                     FGVPYLEPMMKPKELVRDVIWRSPEYTFQRRPEYPSPQDQIRQKAFMRTWIPEVAEKA
                     RQDGEALADSRDQSGEEENTRGRG"
BASE COUNT         1489 a         2088 c         1470 g         1408 t
ORIGIN      
        1 ccactccgaa gcatcgtccc ccgcctctcc cagagaaccc gtcacgatgg gctcagccat
       61 ctccacttct cgtccataag cccgttcttc ggcagcctcc tgcgccaatg aacgtctcgt
      121 tttcggcagc ggccacgcat cgtaatcttc ttccgcttcc acagattcca gttcatacga
      181 ctgcagagtc gatgggactt caggagatcc cgcaaatcca tcagcaggcg ccgacggctc
      241 catgcggccc tcagcctgag catccctgga agcttcaaga tgtcgagcct tcttgagagc
      301 ttcctcctcc gcctgcttga accctctagc gacaatccgc agcacgtaag agaagataac
      361 cactatccat acaaccagcg ctgccaggtc gctcatatcc atacggccaa cacctccatc
      421 cccgctccgc ggcgcacagc caaacaaaag atgctttcac gctgccgtat cgcccggacc
      481 ttgccgtacg catcaggcag cactacttca agccagcatc cgaaccagag ccctcagaag
      541 aacctggagc accagtcgct ttcgagattg aatcccgcat ctgggtgtcg gccttgagat
      601 tgagcaggtt gtagtagtcc atcaccccaa gattacctga gcgcaatgcg tcggcaagtg
      661 cccgcggcac ctccgcctct gcctccacaa ccatcgccct catctcctga accttggcct
      721 tgttctcctg ctcgagggcg agagctgcaa acctgcgctc cgcggccttg gcttgagcga
      781 tgttcttgtc ggcctccgcc tggtccatct ggagccgagc cccgatattc cttcccacat
      841 ccacatcggc aatgtcgatc gaaagaatct caaaagccgt tccgtcatcc aaacccttgt
      901 tcagcaccgt tctggagatc tggtctggat tctccagcac ttccttgtga ctctctgcag
      961 agcccacagt cgtgactatc ccctcgccaa ccctggcgat gatagtcgcc tcgccggcgc
     1021 cgcccacgag tctgtcgatg ttcgctcgca ccgtcacccg agccttggcc ttgagctcaa
     1081 ttccatcctt tgcaactgcg gcaatcaccg gggtctcgat caccttcggg ttcacgctca
     1141 tctgcaccgc ttcaagcacg ttgcggcctg caaggtctat ggccgccgcc ctttcgaagg
     1201 tcaggtttat atcggccctt tgcgccgcga tcagagcatc gacaacacga tccacgtttc
     1261 cgccggcaag gtaatgcgct tcaagcttgt cgacggtcac cggaatgcca ccctttgctg
     1321 ccttgaccat tggcaacacg atcctgaccg gcggaacgcg acgcagtctc attccgacga
     1381 gagtgagaag ccccacgttt actcctgctg cccatgccga aatccaaagc cccacgggaa
     1441 tgaagttgag cagcaccagc agaagaacga ggacaacaat caaaattatc cagcctatag
     1501 ccatgcaatc aatcctcctg atccatttgg gtaataggcc tcacgaatac cttgttgcct
     1561 tctatgcgaa tcacccggac gcgggctcct cccataacga aggggccttc ggatatggcc
     1621 tcaaccctgc gaccgtcgaa atccactata cctacaggtt tgagcggggt aaaaaccatg
     1681 ccctcagcgc cgtaaagaga ttcgtacgtc tccacggcta agtatccttc ctccggcgtt
     1741 tcagtgtgat caaggaccaa tttcgccggc gcacgaccct ttcttcctcc acgggagagc
     1801 aagaatatca ccaacgccac aacggctatg gctatgccta tcgctatccc cagagctgca
     1861 gcgccttcgc caggggatgg gtagctaagt acaaagctgg caatcacgct tacaatgccg
     1921 agaatgccgg ccacgccaaa ccctggtatt acaaacgcct caaggagaag caaggtcagg
     1981 ccgaccagaa acagaacaat gacttcccat ccggcaaggc ccgccattat cctgcccccg
     2041 aagaaaaggc cgatggagac gattcctatg gttcccggaa tcccgaaccc cggcgtcacc
     2101 agctctacag ccagacctac aaggcccagc gtgagaagga cctctgcagc aacctgattc
     2161 gtgagaagcc tggcgaacca ttccgctgaa gtctgcttca catcgacgat ctcagcgcca
     2221 gacaggccca atagtccgag aagatcatct aggtccttca ctttgtattc gataaagcca
     2281 agattctccg cagcagtggc tgtgagggta agtatcttac cgcgctcgat cacgccggga
     2341 atctcgacgt ccgcatctac catcgccccg gctacagccg gatccctgcc ccgaagctcc
     2401 gctgttgcct caaattccgc ccgcaccgca gagatgtact tttcctcttt gggtctggtc
     2461 tcagcagcgc ccatcgaact ccccattttc atggcaatat catcacaggc aatcgcgatg
     2521 agagcgcctg cagaccacgc gcgcgaatcg acatacgcta tagtcctaac ttcagaagag
     2581 atgatggcat cgcgcatctt aagcgcggaa tcaacataac cgccaggcgt gtcaatgtcg
     2641 attatcacga catccgctcc cgcatcggaa gcttctcgta gccctcgcgc tatgaaggac
     2701 gccaatccgt gctcgatctg cccctgaagt ggaacgatat atactttagg agctgcggcg
     2761 cacttgccag cagggaccag taaccccacg acaatcagcg caagaacgaa aacccctatt
     2821 acgcctcttt tcgctctcaa gcaggagtcc ctcccctctc ttatgtcaaa tggaataatg
     2881 ccgatcccaa gcagcgtcgc aaaggcgaag aagccgggaa acagcgcagt cgccctcgcg
     2941 gctcaggcgc gggtttgcat gaagctattc ctcgccgcct tgggcgtcaa gcatctctct
     3001 gaccatactg tttaccagct tcccgtcagc acgccctctc agtttaggca tgactgcgcc
     3061 catgactttt ccgatatccc gcggttcctc tgcgccggtc tgagcgatcg tttccgagat
     3121 gatctgcctt agctcatcct ctgatagttg tcgcggaagg tacgacatga ttatttccat
     3181 ttcttttcgt tccacgtctg cagcatcgaa cctgcccgcc ttttcatact cttctatcga
     3241 ctcgcgcctc atcttcgcct gcctcgccat tacatcgact tcttcagact cagacaacgg
     3301 cgcgcctttc tcaagctctg catttttcaa cccggccaag gccaaccgta tcacggattt
     3361 ttttagctca tctttggatt tcatcgcgat cttaagatcg tccctcaatc gcactgcaat
     3421 cgacattcca atcaccatcc caaaatgcat cgacatcttg attataccat tagccagttc
     3481 tccttgaaac agaagaagcc tcgccgatct cgatcaagcc attacgcttg cggtcggggt
     3541 gaagtcatcg ttgtgccttg cctttccact ttcaggctta cctcgggaat tatcttgatt
     3601 tccttgaata tgtcgcgcca gataggcgcg atttcttctt cccacacatc gctggaaatg
     3661 cgcctcctca ctgcttcccc aattccaaaa atgtccgtcc caagccgatt ctgcgcaatc
     3721 tctatcaatg attccatttc cgcctcaatc tggtccgaaa cgccacgact caagttccga
     3781 tccgcctcta tcgaaacaaa ctgctcctcg gacatcactt cttctatgta tacacaacct
     3841 gttacccgta tccggacttc cgccccgcgc aagcccgtcc tggtcatctt atgtctcggc
     3901 ggaagctcta tcgtggcccg agaatgcaat accttcacgc cgacagtgtc tccttccgtt
     3961 cctggcacca tatgtagaaa tgaatattcc ttcagtctgt ttctagccat gagaaccatg
     4021 gcagtctgca cgggatcaag ccaacccacg accaagtccc catggagggc agcgctcccg
     4081 cttatctcga tgtcgctttt ggtttcaaca tctccctttt taccctttcc gccttcattt
     4141 ccagtccgct gtccccaaac accctgctgc gcctcgtcct gctcaccctc ttcctcctca
     4201 ggttcacttt tcaccagact gactctggca agaacaggct gatctcccgg ttccgcagcc
     4261 gtgctcaaca aatctctgaa cctcgattgc ggagcgaccg acgaaagagt tgcatgacgc
     4321 atcatcaggt tgatgtaaga tgaagccatg ttctcctggt ctgcctcgac ttcgaatatc
     4381 tttccgacag ggtcagatgt caccaatacc cacgagttat agcgaaactg ggagtccctg
     4441 gccagataat ccaggatagg cgtcaagccc ttcctcgcga tcttctcccc aataaccagc
     4501 acctttacat gaccgcgaaa cagcgtcttt gccgatttca ttctcaacgc agacatggcg
     4561 tccatcattg tgtttccggc agcagagcca agccaagcag gggtattggc cgctccctcc
     4621 ggacccgagg acggaagagc catctggacc cagacaacga ttttgccgtc atccgactcg
     4681 tccactccca ttcccatgat gaacccaata tggtcgatat cgcgcgcatc ccaacaaccg
     4741 ccaagagcga aaaccagaat gacgacgcag agcacgcatg ctgcgactgt gagacgtctg
     4801 cccaatggat gcgagccgtg gtcgctcgaa acatcgacat aatccatgtt atctgctgtc
     4861 atctggtgta ccacccttct cgtgccgcaa cacgcgagcg ccgatcagca gcaaggcggg
     4921 aagcgcaatg gcataccaga cagtgtactt caagaaatac tcaactgctg caaatgactg
     4981 agctacattt gcagggatag agctcagcac catcgatatg attccgaatg gaaggatcaa
     5041 tcgcctgtag ttgtccaggt tgagcaggtc tgcaatggcc gttatggctg catagaatac
     5101 tgaagccagc ttcaggatgg caacggagaa ccagagcgcc aggaagagaa tctctcctct
     5161 ttccagaaac tcccctgctc caaccattcg agcggtctct accgcggggt aagtgacgcg
     5221 tgatatcccc cctgtgccca gcatcataat gccgtatgcc actgtcattg ccattatggc
     5281 cgtccctatt gccacacccg ttcccactgc cctggataca tgacgtttgt ccttcaaatg
     5341 cctgagaaat gcgagcgata cgatggcctc gcctgtatag gaggccgtaa gcaaggctgc
     5401 cctcatgatc ggaatcacgc ccctgcccgc caacggcaag agacgccccg gaaccaacac
     5461 aggaatagcg aaaaccccca aaatggcgat ggatatgata accactccga agaagatgaa
     5521 acagacgcgg gctatcgcct cgatgccgag gtaggcagcg tagctcgcca ggataaagaa
     5581 gacagcggta gttacgtaga atggcgttct ttccagaaac gagatagata tagcgagcac
     5641 gaattcccgt gtaccaagca cgaccacgac agtgcaatag agtgcgaagg tgaggccgat
     5701 taccttgccg aatggtttgc cgaatatgtc ttctgcgatc tttaccagat tctgttcagg
     5761 aaaggccgat gagaccgatc ccaccagcca tgcaattacc aaccctatta cgccgccgag
     5821 caggaccgat atccatgctg ccgtgcctgc tacaggcgtc aacaacctgg cggctgcaag
     5881 aaaccccgtg gaaaacacaa tgaccgcaac gagcacaacc acctgcgtgt agtaaatctc
     5941 atcctttgca cttccgccta ttgtccatgc acctctcgtc cgttatgtct aggtttcatc
     6001 attgccatag cgactgccac cagcgcagca ctcccgaccg gaggtgccac ccttgctgac
     6061 gcgatcgcct tatcagcccc gtcccctcgt attctcctcc tccccagact gatctcggga
     6121 atccgcaagc gcctccccat cctgtcgcgc cttctcggcc acctctggaa tccatgtccg
     6181 catgaaagct ttctggcgta tctgatcctg tggcgacgga tactcaggac gcctttgaaa
     6241 cgtgtactcg ggcgaacgcc atatgacgtc gcgtaccaac tccttcggct tcatcattgg
     6301 ctccagatac ggaacgccga aagatgtcag gcccccaagg tgcaaggcaa tcgctattag
     6361 cccggcgatc agcccaaaaa gcccgagact ggccgaaaga aaaaggagcg gaaagcgcag
     6421 aagcctgatg gccaaacctg taggatagtg cggga
//
LOCUS       DAOSAI010000094         6411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7723_length_6411_cov_3.512115, whole genome shotgun sequence.
ACCESSION   DAOSAI010000094 DAOSAI010000000
VERSION     DAOSAI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7723_length_6411_cov_3.512115"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..309
                     /locus_tag="PLM83_04505"
     CDS             121..309
                     /locus_tag="PLM83_04505"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70703.1"
                     /translation="MRKTLPREEDVRYPICTGGRRACPPEDIGGIYGYYSYLEALADP
                     NPEMHGDAVELSIAVLSL"
     gene            561..797
                     /locus_tag="PLM83_04510"
                     /pseudo
     CDS             561..797
                     /locus_tag="PLM83_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016766698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(958..1806)
                     /locus_tag="PLM83_04515"
     CDS             complement(958..1806)
                     /locus_tag="PLM83_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HOK70704.1"
                     /translation="MSLRSMTELLDTVEVGYAVIAFNFIGLDDLKGVMKAVERNQTPV
                     ILQVSQGTAAHLGLDYAVALARVAANVSAVPVCLHLDHATSIELILKAISSGFTSVMF
                     DGSRLPFEQNLEMTARVVEMAHDVGVSVEAELGNIGGKEDDPQGNGDGQVLVEPSQAC
                     EFVAATGIDALAPSVGTAHGLYKGTPDIRFDLISSLVELIDVPLVLHGGSDIPDAVLQ
                     KAIRLGMRKVNVGTDLQVAHVQALREALAETSGLPDMRKVHSRVIDAVCAMAESKIRL
                     GGLGRH"
     gene            complement(1899..2843)
                     /locus_tag="PLM83_04520"
     CDS             complement(1899..2843)
                     /locus_tag="PLM83_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOK70705.1"
                     /translation="MIATFTLNAAIDRTLRVESLEKGIVNRATLTAARAGGKGINVAS
                     VAHSMGQKVIVSGFVGGHAGQFIEDYITSVGMVHDFVQIEAESRNCVAILESSTGTIT
                     EVNEAGPEVEYGALCAMEKKVEYLAERCGVCVFSGSLPRGCPQDYYARLIKIAKKQGA
                     FVVLDTSGSALCAAIEAKPDLIKPNEYEIQDLLGVEVSSEPQLIEALDEIRSRWSIDH
                     VIITLGPHGAICSIPTGFLRVLVPKLPVVNTVGCGDAFVAGICVGHERNLPAKEFIRL
                     GAAAASAKLGAKDAGTCDPEEVRRLVGEVQVEDIGQMG"
     gene            complement(3000..3728)
                     /locus_tag="PLM83_04525"
     CDS             complement(3000..3728)
                     /locus_tag="PLM83_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK70706.1"
                     /translation="MSIDTESPMPLYYQIQRELTERIVRGDYSDSGKLPSESQLQAEF
                     NVGRATIRKAIDELAKRGLVVKHHGKGVFLAPPKMEQHLLSFYSFTDEMIDKGFTPST
                     EVLEFEYMTTLASVARALGLQKGTYVIRIVRVRSADDEPLIYETSYLPASLVPGLTRE
                     RVSDMPLYEIIEKDYGLRLSRAVESFEPVNLGPYEARILGVPAGIPALFLERTAYSDT
                     IPVEFCQGLVRGDRCRYQVELERP"
     gene            complement(3809..4894)
                     /locus_tag="PLM83_04530"
     CDS             complement(3809..4894)
                     /locus_tag="PLM83_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOK70707.1"
                     /translation="MDYILKEILEESEAARTTIEKGDASQRQAAHAVLAADPDAIYAV
                     GCGSSYFAGMMARYPIEGFVHIPTIALSATDFITYSLDTITDASVVIGISQSGESFET
                     VEAIEKAKDRGALLVGLTNNPTSSIGHIADELILTYAGEEKASGTKTVLAQMLAAYQF
                     ALAMAELKSDIVSEAHRVLANELDKMPDTIGKLLERRDDAIRLAKELKDDSNLYLVGA
                     GPFYPLGLQIANTAREIARVHVNAFDVVEFRHGPLEIVESGTPVVFISNSYSPLRDNI
                     ATLASLVKRAGGRVIAATDESDVELNRAADIQVTMPSMSEIAGAIAYLSFFHMFLYNL
                     AVEKGLNPNEFRNIVKTWTQEPGEQEH"
     gene            complement(4904..5884)
                     /locus_tag="PLM83_04535"
     CDS             complement(4904..5884)
                     /locus_tag="PLM83_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOK70708.1"
                     /translation="MPSRGVKEYDRRIAAVGNYNIDMVVGRVENLPTWGTEATVPYLQ
                     VRPAGSAGYTTIALATVGVGVTCIGNVGEDSYGVFIRDELAARGADVSGLLEADGADT
                     GQSVVIVRRDSERAFLSYNGHLAAFDEKCLMEAETAAVGFEQTEWVLLSGYFLLPGLG
                     YEATKRAFTRWKAMGKRVAFDPGWDTEGWPQHTVDEVTKLLQFVDLFLPNEDEARALT
                     QGSNPCSMASTLRDAGAGIVAVKLGADGAYVFDGEERIHMPAIKTEAFDATGAGDSFN
                     AGVLTGLTKRWTWNRTLKFANTLAGIVVSRKANRFPKLDEVYKKMKSQEE"
     gene            complement(5891..>6411)
                     /locus_tag="PLM83_04540"
     CDS             complement(5891..>6411)
                     /locus_tag="PLM83_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK70709.1"
                     /translation="EFRGREQMLLGIVAVKMLPAISTMVALYVLLSKVQLLDTRTGLV
                     LVYLSFTLPFAIWIMAGFFQTIPKELEEAARIDGTSRLGALFHVILPLSAPGLVSTAI
                     FVFMMSWDEFFYPLIFTSTYAAITEFAGRHAIDYSAMAAGGVLASIPPIVLAFIFQRY
                     IISGLTAGGIKG"
BASE COUNT         1573 a         1869 c         1415 g         1554 t
ORIGIN      
        1 acatgtggga tcctccgatc gaggagtctg acgtgacaat agccgatgcc gccatggcct
       61 ctggaggcaa gttccggtat acttacgatt ttggagacga ctgggttcac agaattactg
      121 ttgagaaaaa cgcttccccg cgaagaggac gttcgatacc cgatatgtac aggcggcaga
      181 cgcgcctgtc cgcccgaaga catcggcggt atctacggct attattccta tcttgaagcc
      241 ttggcagacc ccaacccgga gatgcatggg gacgccgtcg agttgtcaat agcagtcttg
      301 agtttatgag aaaacagatg aagtgagact agtcaggcct gaatgatcct attcagcgct
      361 gccacggtcg ctgcacttta ccggataggc cgaggagcag agctttttcc ttattcccat
      421 gatgctcatg tcctcctcta tctttcctgt attgtccatt atcgccatga ctccaactca
      481 attctcaaat cactgtcgag agacgcttac agtgttggca aaaccctgtt cgtggatgag
      541 atgggcttct gggccaatca atcgccagct gctatgagcg ccaaagcgtg gccatcacca
      601 cgaaccttga gttcggacaa tggaacacgg tgattaacga caatcggctt accggcgtgc
      661 tcatcgatgg gtttgttcat catagacatg tgcttgcatt cacaggcgag agctaccgtc
      721 cacggcatgc cctctcccaa ttgcatgccg cagatgacgt cgggggcgcc cacctccaac
      781 aaggcactat ttgctagaac ggactaatcc cgtagggcct cgaatttcga ccccgggcgt
      841 agtggttaac ctatgcactt ttgactgccg aactatgaac ttttggattg ccaaattgat
      901 tttactcccg gagcacacca gacgatgaga aaaccttatg ccaatgacat ccggaggcta
      961 atgcctccca agtcccccca aacgtatctt gctttcagcc atggcacata cggcatcgat
     1021 gacgcgtgaa tgaacctttc gcatatccgg aaggccagat gtctcagcca aggcttctct
     1081 cagggcttga acgtgagcta cctgaagatc agttcccacg ttcacttttc tcattccgag
     1141 cctaatggct ttctgcaaga ctgcgtccgg gatatccgac cctccatgca acaccagtgg
     1201 aacatcaatg agctcaacaa gtgaggatat cagatcaaac ctgatgtctg gcgtaccttt
     1261 gtaaagtccg tgagcagtgc caaccgacgg cgctagggca tctatgcctg tagctgccac
     1321 gaactcgcag gcctgggatg gctcgacaag aacttgacca tctccatttc cttgcgggtc
     1381 gtcttctttg ccgcctatgt ttccaagctc cgcctcgaca gaaactccca cgtcatgtgc
     1441 catctcaacc actcgcgcag tcatctcgag gttctgctca aagggcagcc tggatccgtc
     1501 gaacatcact gaggtaaacc cgctcgatat ggcctttagg atcagttcta tgcttgtcgc
     1561 atggtcaaga tggaggcaga ccggcactgc ggaaacattg gctgcaaccc gggcaagagc
     1621 aacagcataa tccaggccga gatgggccgc agttccttgg cttacttgaa gaatgactgg
     1681 cgtctgattc ctctctacgg ctttcatcac gcccttcaaa tcatccagac ctataaagtt
     1741 gaaagcaatt accgcatacc caacctctac agtatcaagc aactccgtca tgcttctgag
     1801 actcatatcg ccacccccta ttgttcagcc accggtagca aatagacaga atgattggcg
     1861 ccaacgtcac gaggtgcccc ttgagcttcg cagtctcctc accccatctg accaatgtct
     1921 tccacttgca cctcgccgac caaccgtcgg acttcctctg gatcgcatgt gcctgcatcc
     1981 ttcgctccga gcttggctga tgcagccgcg gctcccaatc gtatgaactc tttcgcaggc
     2041 agattgcgtt catgcccaac acatattccg gcaacgaatg catcgccaca gccaacagta
     2101 ttgaccacgg gtagcttcgg cactagcact ctcagaaatc cggtaggaat ggagcaaatg
     2161 gcgccatgtg gccccaatgt aataattaca tgatcaatcg accatctgct tctgatctca
     2221 tctaacgcct caatcaactg gggctctgag ctcacctcaa cccccaaaag gtcttggatt
     2281 tcgtattcat tgggcttgat cagatcgggt ttggcctcga ttgcggcaca aagagccgac
     2341 ccactagtat cgagcactac aaacgcccct tgtttcttgg ctatcttgat caatcgggca
     2401 taataatctt gaggacagcc ccgcggaagg ctcccggaaa agacacacac tccgcacctt
     2461 tcagccaaat actcaacctt cttttccatg gcgcagaggg caccatattc cacctccgga
     2521 ccagcctcgt tcacctctgt aatcgtccct gtgctcgact cgagtatggc tacgcaattc
     2581 ctggattcgg cctcgatctg cacgaaatca tggaccattc caaccgaagt aatgtaatct
     2641 tctatgaatt gtcctgcatg cccgcccaca aagcccgaga ctatcacttt ttggcccatt
     2701 gaatgagcga cactggctac atttatcccc ttgcctcctg ccctggccgc tgtgagggtt
     2761 gccctgttca caatgccttt ttcaagggat tccaccctca aggttctgtc gatagccgcg
     2821 ttcaaggtga aagtcgcaat cactcataca atcctccttc aaagcgaact cgcatggcga
     2881 tcgagcagtc agacgctcaa ctagacggct gcgaaagaca catgctccac aggacatcgc
     2941 atagttcgca gtagcatata ctcacacagc attcccatag cgtcagagct gaagctccat
     3001 cacggtcgtt ccaattccac ttggtaccga cagcggtcgc ccctcacaag accctgacag
     3061 aattccactg ggatcgtatc cgaataagcc gttctctcca gaaacaaggc aggtatcccc
     3121 gcaggaacgc ccaagatacg cgcttcgtac ggtcccagat tcacaggctc aaaagactca
     3181 actgcgcgag atagcctcag tccatagtcc ttttcgatta tctcgtacaa tggcatgtcg
     3241 ctaactcgtt ctctcgtcag acccggtacc aatgaagctg gcaagtatga ggtctcgtat
     3301 atcaacggct catcatctgc cgaacgaact ctgacaatgc gtatgacata agttcccttc
     3361 tgaagcccca atgctcttgc cacgcttgca agcgtagtca tatattcaaa ttccaacact
     3421 tcggtgcttg gagtgaatcc cttgtctatc atttcatccg tgaagctgta gaatgacaat
     3481 agatgctgtt ccatcttggg gggagccaaa aaaacccctt tcccatggtg cttcaccacc
     3541 agtccccttt tagccaattc gtcaatggcc ttgcgaatag tggctcttcc tacattaaac
     3601 tccgcctgta attggctttc agagggcagt ttcccgctat cactgtaatc acctctgact
     3661 attctctcag tcaactcccg ttgtatctga tagtaaagtg gcatagggct ttctgtgtct
     3721 atagacacgg ctacattccc cctttggtcg agcgcatgcc cgtcaaagtg ggcacagcgc
     3781 atgtccgcat gcaacatgcc atatctatct agtgttcttg ctcaccgggt tcctgagtcc
     3841 aggtcttaac aatattgcga aattcgttcg gattcaaccc tttctcaacc gccaggttgt
     3901 ataagaacat gtggaaaaat gacagatacg caattgcgcc agctatctca ctcatggaag
     3961 gcatggtaac ctgaatatcc gctgcacggt tgagttccac atcagattcg tcagttgcag
     4021 caattactct accaccagcc cgctttacta acgatgccaa tgtggctata ttatcacgca
     4081 acggagaata cgagttactt atgaatacga caggtgtgcc tgactccacg atctcaagcg
     4141 gcccatgtct gaactctaca acatcaaagg cgtttacatg aactctggct atttctctag
     4201 cagtatttgc gatctgcaat cccagaggat agaatgggcc agctcctacc aggtacaagt
     4261 tggagtcatc cttcagctct ttcgcaagtc tgatagcgtc atcccgtctt tccagcagtt
     4321 tgcctattgt atctggcatt ttgtccaact cgttggcaag tacgcggtgg gcttctgaaa
     4381 caatatcact cttgagctcg gccatagcca atgcaaactg gtaggcagcc aacatctgag
     4441 caagcaccgt tttcgtgcca gatgcctttt cctcgcctgc atatgttagt atcagctcat
     4501 cagcaatgtg accaattgaa cttgtaggat tattcgtaag gccaacaagc agagctcccc
     4561 tatccttcgc cttttctatg gcctccactg tctcaaaact ctcccctgac tgggatatgc
     4621 ctatcacaac cgacgcatcg gtaatggtgt ccaatgagta tgtgatgaaa tccgtagccg
     4681 acaacgcaat agtgggaatg tgaacgaacc cttcaatagg gtatctcgcc atcatcccag
     4741 caaaatagga tgacccacat ccaaccgcgt agatcgcatc aggatctgca gccagtaccg
     4801 catgtgccgc ctgcctctga ctggcatccc ccttttcgat agttgtgcgt gcagcctctg
     4861 actcctcaag aatttcctta agtatgtagt ccatgtagaa cgttcactcc tcttgtgact
     4921 tcatcttctt atatacttca tctagtttag gaaatcggtt agccttgcga ctcacaacta
     4981 tcccagccag tgtattggca aatttgagtg ttctgttcca tgtccatctc ttggtcaaac
     5041 ctgtaagcac accagcattg aatgagtctc ctgcgccagt agcgtcaaac gcttcagtct
     5101 tgattgcagg catgtgaatg cgctcttctc catcgaacac atatgcgcca tcggcgccca
     5161 gtttcacagc cacaattcca gcaccagcat cacgaagagt cgacgccatg ctgcacggat
     5221 tggatccctg tgtaagcgct cgcgcctcgt cctcattggg cagaaagagg tccacaaact
     5281 gaagaagctt agtcacttcg tcaaccgtat gctgtggcca tccctcagtg tcccagcccg
     5341 gatcgaatgc cactcttttg cccatcgcct tccatcgtgt aaaagctcgt ttcgtcgctt
     5401 catatcccaa accaggaagg agaaaatatc ctgatagcaa cacccattct gtctgctcaa
     5461 aaccgaccgc agccgtttcg gcttccatta ggcacttttc atcaaacgca gcaaggtggc
     5521 cgttgtagct aagaaaagcc ctctccgagt cacgccggac tatcacaacg ctttggcctg
     5581 tgtcagcccc atcggcctcc agcagcccac tcacgtcggc tccacgcgct gcgagctcat
     5641 cacggatgaa aacaccatag gaatcctctc ccacgttccc aatgcacgtt acgccaacac
     5701 ctacagtagc tagggcaatc gttgtatatc ccgctgaacc tgcaggccta acctgcaaat
     5761 atggcactgt ggcctcagtt ccccacgtcg gaaggttttc aacacgtccc acaaccatat
     5821 ctatgttata gttgccaact gctgcaatcc ggcggtcgta ctctttcacg ccacgacttg
     5881 gcaatatcat ctaccccttg attcccccag ccgtaaggcc agaaatgatg taacgctgga
     5941 atataaacgc taatacaatg ggaggaatac ttgcaagcac gcctcctgca gccatggcag
     6001 aatagtcgat ggcgtgccta cccgcaaatt cagtgatcgc tgcgtacgtg ctggtgaata
     6061 tgagcggata gaagaactcg tcccacgaca tcatgaacac gaatattgcc gtagaaacca
     6121 agcctggtgc tgagagcgga aggataacgt ggaaaagggc acccaaacgc gaggtgccgt
     6181 cgatacgtgc cgcttcctcc agttccttgg gaatcgtctg gaaaaaacca gccatgatcc
     6241 atatggcaaa aggcaacgtg aagctcaggt agaccaggac caaaccggtt ctagtgtcga
     6301 gcagctgcac cttgctgagc agcacataca aggctaccat agtagaaatg gcaggcagca
     6361 tcttcaccgc cactattcca aggagcattt gctctcttcc ccgaaattcc a
//
LOCUS       DAOSAI010000095         6350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7810_length_6350_cov_4.288642, whole genome shotgun sequence.
ACCESSION   DAOSAI010000095 DAOSAI010000000
VERSION     DAOSAI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7810_length_6350_cov_4.288642"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            890..1360
                     /locus_tag="PLM83_04545"
                     /pseudo
     CDS             890..1360
                     /locus_tag="PLM83_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1569..3092)
                     /locus_tag="PLM83_04550"
     CDS             complement(1569..3092)
                     /locus_tag="PLM83_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOK70710.1"
                     /translation="MAIWAFLVGVSNYWPKNYNLPFCRNDIIAMRRALESGLRVRPEN
                     IVSLGEAGTVNAKLFSDELVDLGSRVAEDDIFMLYFSGHGGSGQACYYLQFSDTIVSA
                     QDIICSLERIRAKTKIIVLDCCMAGDFSLDRSVSLDIHHAVEELVGKGYAVLASSKAD
                     QLSWGHPGKEMSLFTAFLCDALEDKHIVRHGKKSLNDICRLVSLYLDLWNKRNPDRQQ
                     HPVFRRSMVGTVYFDVEEYTHYCRNRIFEETERYIIYDVEPTNSSSAKRYSAKVILKE
                     PFSISEIGDISTDIKNRLLYEEVHKNEISERRWRGKPANIIWIYYGRDEEDMIHGHFL
                     CRVTWVDEQQDKTWWYRCYQHDFVDKGLHFTLYPYYETLRNLRNNHAVPAEVFAAQSR
                     DIIAELISLAEEAISIYNQFQNEDISEGELFDKMDSLIPKIEEVYTREGELGYAPPEL
                     HELQTCCSGLAAAVHSFTLYYNKRYAEQTSSRNRRECMNLAVQDYYVWLERVKLLRS"
     gene            complement(3154..3618)
                     /locus_tag="PLM83_04555"
     CDS             complement(3154..3618)
                     /locus_tag="PLM83_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HOK70711.1"
                     /translation="MDVFDKEKRSLVMSRIRSVGAKSTELKLRSMLEEAGYSHWQEHD
                     PSLPGRPDFSFPEAKVAVFVDGCFWHGCPHCHDGHIPKSNCAYWSGKIARNKRRDRRV
                     STQLRRLGWTVIRVWECRLKENPARELKRILRKLDTRAYRGEIMARGDNHSS"
     gene            complement(3621..4481)
                     /locus_tag="PLM83_04560"
     CDS             complement(3621..4481)
                     /locus_tag="PLM83_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoMIV family type II restriction endonuclease"
                     /protein_id="HOK70712.1"
                     /translation="MVLNEMNTLRQQYHREISQRLLSVRDGVVNIADRSSESSRQISA
                     RLASKIGGPHCATPPSAQTAGALFGTITKEYLERSFRLLNRLRPGPWIFSTERSISDF
                     DQYRHLAELNEILNTRRELMATLGPDYIIKPDIVVCRIPVADEDIGVEGDTPFIATHT
                     PLRRVNRSGDLVPILHASISCKWTMRSDRAQNTRTEALNLIRNRKGNSPHIVAVTAEP
                     LPMRIASLALGTGDLDCVYHFALYELEEALDEVSHANPGVRDQHEMLQTLIEGRRLRD
                     ISDLPFDLAV"
     gene            complement(4438..5550)
                     /locus_tag="PLM83_04565"
     CDS             complement(4438..5550)
                     /locus_tag="PLM83_04565"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOK70713.1"
                     /translation="MYHRNRKRCTYELGYDSIQMGCYSLFSGPGGLDLGFEMAGASIL
                     YAADIDPVCVETYNYNRPENPVARIRDLSVVSVESILAEALPAGPYDVVGVIGGPPCQ
                     SFSYANVYSTDEDPRHKLPEHYARIVAGLRERVGLDFFLFENVPGLMSASHIAKFKKF
                     EAMFEEAGFRLFQGSLDAQDFGVAQIRPRVFVVGFNKEKYPDIEFRFPVPDPLVPRKT
                     VRDVIWGLPEPVYNAKGLNPDTFPVHPNHWCLVPRSPKFSNGLSKRMGGKSFKILDWD
                     RPSYTVAYGHREVHVHPNGHRRLSIYEAMLLQAFPKDYVLKGNISAQVRQVSEAVCPV
                     VANALAKSIMQALNQERKENIGNGSERDEHFAAAIP"
     gene            complement(5870..>6350)
                     /locus_tag="PLM83_04570"
     CDS             complement(5870..>6350)
                     /locus_tag="PLM83_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOK70714.1"
                     /translation="IEYTPKLRDKALIRFLYSTAVRVEEAHKMCWKDINNGVLTVHGK
                     GGKNRFIRLTEETLELLESIRPEDWQPDDPVWVSQKGNPLSTCRMWAIVKEAAKRAGI
                     KKDVSPHFMRHSHATHALDRGAPIHLVQAQLGHASLATTSKYVHVRPDDTSSRYLPI"
BASE COUNT         1635 a         1675 c         1438 g         1602 t
ORIGIN      
        1 ggtttacggt gttgtgcagg tatgccgtcg cgtcctccag gcgcacccgc cattgagccc
       61 ctaccttagt ggctggtata gtcccgttcg ttatcaactt gtgggccgtg ttatagtgaa
      121 cctgcagata cttcgctaag gcagcaacgc caatgaggta ctcttcgttt cccatggcta
      181 cacctcacat tcacatttca catccgttca cacgatcact tctacccaca attgggaagt
      241 cctgcaagca agaagaaaaa agagtgttag cggccagctt agaatggaca taaatggcca
      301 cgaaaattcc ccacgaatgg ccagatgaag aaagagaaag gctcacccac tcgaattgga
      361 ttggtgactc caaccaatgc acagattcga ggggtgagca agcttgacga aagtggggaa
      421 aatcgtgcag ataagagaat tgcgtaacat gggattgacc aaaaaggcgg tagctcggcg
      481 cttggggctc gaccgtaaga ccgtagccaa gtactgggac ggcccggctg aggaccccga
      541 gaaaactcgg taccggcagc gttcgaagtt gacagatccc tacttggagt atatcacaga
      601 acgcttgaaa aagcttcctg agttgacggc agaacgcatc taccgcgaga tcaagaaact
      661 gggatacaca ggttcgaaga gaactgttag gcgctgtgtt gcgactctcc gccccgaagg
      721 ctttcgcgag ttcaaaccca ttgaaacgct tcccggtgag caagcccaag tagactgggg
      781 ccactgtggg aaaatagacc ttggcggctg caaaatcccg ctttacgtgt tcgcgttcag
      841 cttgtcttgg tctcgagtac gttatgtcga gttcattact tcgctcggga tggagttcgt
      901 tagagcacgt gaaaacgtag ttctgttggg gcctcctggt gtaggtaaga cccatctagc
      961 agtttcactg ggcattgagg cgattaacca gggctaccgg gttcagttca tcacggctca
     1021 ggacctgtcg gacttactct acagtgctct cgctgatggt acggtcacgc aaaccatgaa
     1081 caagctactg aggaacgacc tgattatcct cgatgagctg ggctacgtgc aattcgacac
     1141 gacgggatca gatcacttat tccagatcat cgcaaaggca tatgagaatc gttcgttgat
     1201 cgtcaccagc aaccttgact ttgaggacta ggggcgtctg ttcgaccagt cggcgacggc
     1261 cgcagcattt ctggacagac tgctgcatca cgcccacgtg ataaccttga ggggcgacag
     1321 ttacagagtc cagtaccgct tgacaccacc actatgttag acaagcaaac agctcgtaag
     1381 gggggtgacc gctacgtaga ccaatcgtat ggctgactat gcccgtaatt caccagctca
     1441 acacagtctg atcgtcttac accgtgagga aatttcatgg ccaaaaaagg ggatcctcaa
     1501 gtggccattg acacatagat aggaagatgg cgataggtga gtcgcaaatg tcggcctcaa
     1561 gatcacactc atgatctaag gagctttacc ctctctaacc agacgtaata gtcttggaca
     1621 gccaggttca tacactctct tcgattcctg gacgacgtct gctcagcata tctcttgttg
     1681 taatacaaag tgaaactgtg aacagcggca gccaaacccg agcaacatgt ctgtaattca
     1741 tgaagctccg gcggagcata cccaagttca ccctctctag tgtacacctc ttcgatctta
     1801 ggtattaggc tgtccatctt gtcaaaaagc tcaccttcgc tgatatcctc attttggaac
     1861 tggttataga tggaaatagc ctcttcggct aaggagatta actctgcgat tatgtctcga
     1921 ctctgcgcag caaaaacctc tgcggggacc gcatgattat tcctcaaatt tctcaacgtc
     1981 tcatagtatg gatagagggt aaaatgcagt cctttatcca caaaatcgtg ttggtagcat
     2041 ctgtaccacc aagttttgtc ttgctgttca tcgacccatg taacacggca caagaaatga
     2101 ccatgtatca tgtcctcttc gtctcggcca tagtatatcc agataatgtt ggcgggttta
     2161 cccctccagc gtctctccga aatctcattc ttatgaacct cttcataaag cagtctgttc
     2221 tttatgtcag tagatatatc cccaatctcg gaaatcgaaa acggttcctt aagaatgacc
     2281 tttgcggaat atcgttttgc gctgctagag tttgttggtt caacgtcata gataatgtac
     2341 cgttccgttt cttcgaaaat ccgattgcga caataatgag tgtattcctc cacatcgaag
     2401 tagactgtac ctaccatact tcttcggaaa acaggatgct gctgcctatc tggattccgt
     2461 ttattccaca ggtcaaggta aagagaaact aatctgcaga tatcgtttag cgactttttt
     2521 ccatgtcgca ctatatgctt gtcttccaga gcatcacaca gaaaagcagt aaacaaactc
     2581 atttctttgc cggggtgccc ccacgataat tggtctgctt tacttgacgc cagaacagca
     2641 tatccttttc ctacaagctc ttccacagcg tgatggatat caagtgagac agaacgatcc
     2701 aagctaaagt caccggccat gcagcagtcg agaacgataa ttttggtctt agctcgtatt
     2761 ctttcaagag aacatatgat atcttgggca ctaacaatag tatcactaaa ctggagatag
     2821 tagcaggctt ggccgctgcc accatgtcct gaaaagtaca acatgaagat atcatcttcg
     2881 gccacacgag aacccaagtc gaccagttca tcagaaaaca gctttgcatt aactgttccc
     2941 gcttctccca aggaaacaat gttttctggt cgtactctca ggccactctc caaggcgcgt
     3001 ctcattgcaa tgatatcatt gcggcagaat ggcagattat agttcttcgg ccaataatta
     3061 ctgacaccaa caagaaaagc ccatattgcc atcgatcgac cctcacgatc ttcctttgat
     3121 aacctctggt ttagacctgc gaagggttct acatcaactc gaatggttat cgcctctcgc
     3181 catgatctca cctcgataag cacgtgtatc aagttttcga agaatccgct tgagttctct
     3241 ggccgggttc tcctttagcc gacattccca tacgcgtatt actgtccagc caagccttct
     3301 gagctgtgta gatacacgcc gatccctccg cttattgcgc gcgattttac cagaccaata
     3361 cgcgcaattc gacttaggaa tatgcccatc atggcagtga ggacagccat gccaaaagca
     3421 cccatcgaca aagacagcta ccttagcttc tgggaaggag aaatctggtc tgccaggaag
     3481 gctcggatca tgctcttgcc aatggctata tccagcctct tcaagcatac ttcgcaattt
     3541 taactcggta ctttttgccc cgacactcct aatgcgagac atgacaagtg accgcttttc
     3601 cttatcaaag acgtccatag ttacacagcc aagtcgaaag gcagatcgct gatatctcta
     3661 agccgtctgc cttcaattag tgtttgcagc atctcatgct ggtcacgaac acccggattt
     3721 gcgtggctca cttcatcaag ggcttcctca agctcataca gagcaaaatg atatacgcag
     3781 tccaaatcgc cagttcccaa ggcaagagat gctatcctca ttggcaacgg ttcagccgtt
     3841 acagcaacaa tatgagggct gttccctttt cgattccgga ttaaattcag cgcttcagtt
     3901 ctggtgttct gtgctcgatc agagcgcatc gtccatttac aggatatgct tgcgtgtaag
     3961 atcggcacga gatcaccaga acgattgacg cgccgtaacg gtgtatgcgt agcaataaac
     4021 ggtgtatctc cctcaacgcc aatatcctca tcagctacgg ggatgcggca caccacgatg
     4081 tccggcttaa ttatgtagtc tggacccaga gtggccatca attctctacg ggtgttgagg
     4141 atctcgttca actcggccaa atgccgatac tgatcgaaat ccgatatcga acgctcagtg
     4201 ctaaatatcc acgggcctgg tcttagccgg ttcagcaacc taaacgatcg ctcaagatat
     4261 tccttggtta tggttccaaa aagggcccca gctgtttgag cagatggtgg cgtggcgcaa
     4321 tgggggcctc caatcttgct tgctaacctt gcagagatct ggcggctcga ttcactagac
     4381 cgatcggcta tattgacgac accgtcacga acagacaaaa ggcgttgaga tatctctcta
     4441 tggtattgct gccgcaaagt gttcatctcg ttcagaacca tttcctatat tctcctttcg
     4501 ctcctggtta agcgcctgca taatggactt tgccagagca ttggcaacca caggacacac
     4561 tgcttccgac acctgacgga cctgcgcaga gatattcccc ttgaggacgt agtccttagg
     4621 aaatgcctga agaagcattg cttcatatat gctcaaccgc cggtgtccgt ttgggtgcac
     4681 atgcacttcc cggtggccgt atgcaaccgt gtacgaaggc cgatcccaat cgaggatctt
     4741 aaatgacttt cctcccatgc gcttactcaa accattgctg aacttaggcg accgcggcac
     4801 caagcaccag tgattaggat ggacaggaaa tgtatcagga ttaagccctt ttgcattgta
     4861 gactggttca ggtagacccc atatgacatc tcttaccgtt ttgcgcggca ctaatggatc
     4921 tggaacagga aacctgaact cgatatcagg gtacttctcc ttgttaaaac ccacaacaaa
     4981 cactcttggc cttatctgag ctactccgaa gtcttgtgca tcgagtgaac cctgaaacag
     5041 tcgaaagcca gcttcttcga acattgcctc aaacttcttg aacttagcaa tatgcgaagc
     5101 tgacatcagg ccaggaacat tctcaaacag aaaaaagtca agcccgactc tttcccgaag
     5161 cccagcaacg attctggcat aatgctctgg caatttatgc cttggatctt cgtcagtaga
     5221 gtatacatta gcgtaactaa acgattgaca aggaggcccc ccgattacgc cgacaacatc
     5281 gtatgggcca gcaggcagcg cttccgcaag aatcgactct acactaacca cgctcaaatc
     5341 ccgaatgcgt gctaccgggt tttcgggtcg attgtagtta tatgtctcta cacaaactgg
     5401 atcgatgtca gctgcataga ggatgctagc gccagccatc tcaaacccga ggtccagccc
     5461 accaggccca gaaaacaagc tatagcagcc catctgaata gaatcatatc caagttcata
     5521 ggtacagcgc tttctgttgc ggtgatacat ctctaacacc tcaattccct attcgattca
     5581 tagcctttcc gaccccttct ggcaaggcag atggttgctc tgttgtagcc atgcacgaaa
     5641 gtcgcataga cgatgtaatc gaagactagt taagctacgc aaccagccag ttatgctgca
     5701 tacccatctg aatttattcg ggcttagact gatcaccttc aacgcgtgac tctttccgtg
     5761 acaacaacca ctctataacc ctaacgagcg gcatcagatc gccgtcctcg ttgaagacaa
     5821 ctgtgactct cacctatctc tcctccacat tgttcgtttc tcgcccctgt taaataggca
     5881 gatagcgact tgacgtgtca tctggtctga cgtgaacata cttcgaagtc gtggcaaggg
     5941 aagcgtgtcc gagctgcgcc tgcacaagat gaatgggtgc accgcgatct aaagcgtgcg
     6001 tcgcgtgact atggcgcata aagtgcgggc tgacgtcctt cttgattccc gctcgcttcg
     6061 ctgcctcttt gacaatagcc cacatcctgc acgtagagag tggatttcct ttctgagaga
     6121 cccaaacagg atcatcaggc tgccagtctt ctggcctaat gctctccaga agctcaagtg
     6181 tctcctcggt gagcctgata aaccggttct tgccgccttt gccgtgcacc gttaggaccc
     6241 cattatttat gtctttccag cacattttat gagcctcttc taccctaact gcggtggagt
     6301 agaggaatct aatcaatgcc ttgtcccgaa gcttaggggt gtactcaatg
//
LOCUS       DAOSAI010000096         6349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7812_length_6349_cov_3.970130, whole genome shotgun sequence.
ACCESSION   DAOSAI010000096 DAOSAI010000000
VERSION     DAOSAI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7812_length_6349_cov_3.970130"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLM83_04575"
     CDS             complement(<1..191)
                     /locus_tag="PLM83_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018302447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOK70715.1"
                     /translation="MDGNGRWAQQQGKPRLYGHQVGANRAKDIVLAARDRGIGYVTLF
                     AFSTENWKRPISEVQGLFLL"
     gene            complement(348..869)
                     /gene="frr"
                     /locus_tag="PLM83_04580"
     CDS             complement(348..869)
                     /gene="frr"
                     /locus_tag="PLM83_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOK70716.1"
                     /translation="MKEVVESTSRDFAGVRTGRANPALLDRIVVDYYDTPTPLTQIAS
                     VSAPEPRLLVVTPWDKSVLGKIEKAIQKSDIGLVPTNDGNVIRMAIPHLTEERRRELV
                     KLVRKMAEEKRISIRGIRRDAIEEFRVLEKDGIITEDELRRAQDEIQKLTDKYIADID
                     NMLADKEVEIMQV"
     gene            complement(908..1627)
                     /gene="pyrH"
                     /locus_tag="PLM83_04585"
     CDS             complement(908..1627)
                     /gene="pyrH"
                     /locus_tag="PLM83_04585"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOK70717.1"
                     /translation="MERPKYSRVVLKLSGEALAGERGFGVDIDTVRWIAGQVSEAYAL
                     GTQLAIVVGGGNIWRGSEASRMGMDRSSADYAGMLATVINSVVLQEALEDSGIPTRVL
                     TAIEIREVAEPYIRRRAIRHLEKGRIVIFGAGTGNPFFSTDTAAALRAAEIEADVILM
                     AKRVDGVYDRDPLKNPEAKFYEKLSFIDVISRELGVMDSTAASLCMDNGIPIVVFNLL
                     TEGNIKRALMGEPVGTWIGGD"
     gene            complement(1753..2355)
                     /gene="tsf"
                     /locus_tag="PLM83_04590"
     CDS             complement(1753..2355)
                     /gene="tsf"
                     /locus_tag="PLM83_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOK70718.1"
                     /translation="MMEITASMVKELRERTGAGMMDCKRALVETDGDFDNAVTLLREK
                     GLAAASKRAGRETAEGVVESYIHMGGRIGVLIEVNCETDFVAKTDQFRTLARDLAMQV
                     AASRPLYVSRDEVPEDVIERERQIYRTQALNEGKPEKVADRIVEGKLEKFYQEVCLLD
                     QPFIREPEKSVSDLVRETIGTLGENITVRRFARFERGEAL"
     gene            complement(2373..3173)
                     /gene="rpsB"
                     /locus_tag="PLM83_04595"
     CDS             complement(2373..3173)
                     /gene="rpsB"
                     /locus_tag="PLM83_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOK70719.1"
                     /translation="MAVVTMKQLLEAGVHFGHQTRRWNPKMKPYIFTSRNGIYIIDLQ
                     QTVRKLDEAYDFVRETVQDGGTVLFVGTKKQARDTIAEEAQRCGMYWVNERWLGGMLT
                     NFATMRKRVEWMKKLEQMEEDGSFDALPKKEVQKLIAERTKLQKRLIGIRDMKQLPSA
                     VFVVDPRKERIAVAEARRLDIPIIAIVDTNCDPDEVDYVIPGNDDAIRAVRLLTSKIA
                     DAVIEGREGLDQALAEEEQDEGFSADGNNGEFQASAKSDDADEFEDAE"
     gene            complement(3463..4257)
                     /gene="codY"
                     /locus_tag="PLM83_04600"
     CDS             complement(3463..4257)
                     /gene="codY"
                     /locus_tag="PLM83_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HOK70720.1"
                     /translation="MNSLLERTRKLGRLIQSSPGHAGRFEEIAHALAEAVSGQAFVVA
                     KNGKILGHSLPHLTDDDFEVLDSEILGNGYVPQKVNEEFLAIFETAANRRPSNGMRAF
                     CNEMLGGSSESCRLTVAPIYGGGERVGTLLLSKSDGELGENDIMLAEYGATFIGMEIY
                     RSRSEKAEEEARKRAAVQMALETLSYSEQEAVEHIFDELAGDEGLLVASKIADRVGIT
                     RSVIVNALRKFESAGVIESRSLGMKGTYIRVLNDKLFDELDRLRTR"
     gene            complement(4377..5771)
                     /gene="hslU"
                     /locus_tag="PLM83_04605"
     CDS             complement(4377..5771)
                     /gene="hslU"
                     /locus_tag="PLM83_04605"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOK70721.1"
                     /translation="MQDLTPRAIVRELDKYIVGQASAKKAVAIALRNRQRRLALAPEM
                     QDDIIPKNILMIGPTGVGKTEIARRLARLVNAPFVKVEATKFTEVGYVGRDVDSIVRE
                     LVETSVRMVKAEKMSGVQEQAKILGEERLLDALAPLPTRDRSQANPFAALWGMPVGQP
                     QDDRMFESKLASARARREETRERLRRGELEDVIIEIEIDDVQGGMIEIFSGAGVEEMG
                     INLQDLMGEMLPPRRRRRKLPVSEARKILITEEAAKLIDMDEVAEEAIARAEQSGIVF
                     IDEIDKIAVKGQGAGPDVSREGVQRDILPIVEGSTVQTKYGPVKTGHMLFIAAGAFHM
                     SKPSDLIPELQGRFPIRVELSSLTKDDFVRILTEPKNALTKQYMALLATDGVDLVFTD
                     DGVEEIARIADEVNSSSENIGARRLHTILEKVLEEVSFNAPEHSERVVVDADYVRSRL
                     EQLVTESDLSDYIL"
     gene            complement(5787..6317)
                     /gene="hslV"
                     /locus_tag="PLM83_04610"
     CDS             complement(5787..6317)
                     /gene="hslV"
                     /locus_tag="PLM83_04610"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOK70722.1"
                     /translation="MRMTGTTVVAVIRDGKCAMGSDGQVTLGDTVVKHGARKIRKLSD
                     WNVLAGFAGSASDSMALLERFEGKLSEYKGNLVRAASDLGREWRTDRVLRRLEAMMIV
                     TDGNRILLLSGNGEVLEPDDAITAVGSGGPYALAAGRALLRNTDLEAVEIVRKSLEIA
                     SEICIYTNGKINIEEL"
BASE COUNT         1364 a         2006 c         1395 g         1584 t
ORIGIN      
        1 agcagaaaca agccctggac ttcagaaata ggcctcttcc aattttcagt ggaaaatgca
       61 aacaaggtaa cgtaccctat tcccctgtcg cgtgcggcaa gaacgatgtc cttagctcta
      121 ttcgccccga cttggtggcc atataggcgc ggctttccct gttgctgagc ccacctgcca
      181 tttccgtcca tgattatccc tatatgctct ggaagcaaag gggttcctcc cttcggggcg
      241 attcaaggtg acatcgccca tcgcggcatc acgccgagag aagattgaga tcatactata
      301 aaccccctcg aatcgagggg gttgtaagcc ttagcaatgc acaaggctca gacctgcatt
      361 atctcgactt ccttatcggc caacatatta tcgatgtctg caatatactt gtcagtcagt
      421 ttttgaattt cgtcctgagc tcgccgcagt tcatcttcgg tgattatgcc gtctttctcg
      481 agaacacgaa actcctctat ggcgtcacgc cttatccccc tgatgctgat tcgcttctcc
      541 tcagccatct tcctaacaag cttgaccaat tcacgtctgc gttcctctgt aagatgcgga
      601 atagccatgc ggataacatt gccatcattc gtaggaacca gaccaatatc agatttttga
      661 atcgctttct cgatcttacc caaaaccgat ttatcccatg gcgttaccac gagcaaacgc
      721 ggttcaggcg ccgaaacgct agctatctgt gtcagaggtg tcggagtgtc ataatagtct
      781 accactatcc tatccaggag ggctggattg gcacgcccgg tgcggactcc agcaaaatct
      841 cgacttgtgg attcgaccac ttccttcatc cgttcctctg ccagttctag ctcgtccttg
      901 tacacaccta gtccccccca atccacgtcc caaccggctc acccatgagc gcccttttta
      961 tgttcccttc ggtgagcaag ttgaaaacaa caatcggtat cccattgtcc atgcaaagcg
     1021 aagctgccgt ggaatccatc acgccaagct cgcgactgat gacatcaatg aatgataatt
     1081 tctcgtaaaa ctttgcttct ggattcttca gcggatcacg atcgtatact ccatcgaccc
     1141 ttttcgccat caagatcaca tccgcctcta tctcagccgc ccgcaaagcc gctgcggtgt
     1201 cagtcgagaa aaacggattt ccagtacccg ctccgaagat tacgatccgg cctttctcca
     1261 gatgacgtat ggctctgcgc cgtatgtatg gttcagccac ctcgcgtatc tctatggctg
     1321 tcagcaccct cgtcggtatt cctgaatcct caagggcttc ctgcagcact acggaattta
     1381 tcactgtagc gagcatgccc gcatagtctg cactgcttcg atccatgccc atccgacttg
     1441 cctcagatcc ccgccagatg ttacctccgc caacaactat agccaattgc gtacccaagg
     1501 catacgcttc cgaaacttgg ccggctatcc atcttacagt gtcgatatca acaccaaatc
     1561 cccgttctcc tgcaagcgcc tccccggaca gcttcaggac tacccggctg tatttgggcc
     1621 tttccaagat tgtcactccc cgttggattc tatgcgaacg ccaattttcc ttctaaatcc
     1681 gtttcacaca ccacggcggc tatgcgaagg agagaacacc ggacgtgttc tctcctcacc
     1741 tgattgacct tatcaaagcg cctctccgcg ttcgaatcgg gcaaatcttc tcactgtgat
     1801 gttttcaccc aaagtcccta tcgtctcgcg aacgaggtca ctcacggatt tttccggttc
     1861 acggatgaag ggttgatcga gaaggcagac ttcttgatag aacttctcca gcttgccctc
     1921 tacgattctg tcagcaacct tctccggctt gccctcgtta agcgcctgag ttcggtatat
     1981 ttgacgttca cgttcgatca catcttccgg cacttcgtcg cgtgatacgt acagcggcct
     2041 tgaggcagcc acttgcatgg ccaaatcccg cgccagggtg cgaaactgat cggtcttagc
     2101 aacgaaatcc gtctcacaat tgacctcaat cagcacgcct atcctgccac ccatgtggat
     2161 gtaggactca actacaccct ccgcagtctc cctcccggca cgctttgacg ccgcagcaag
     2221 gcccttctcg cgaaggagtg ttacagcatt gtcaaaatcc ccatcggtct cgacaagcgc
     2281 cctcttgcag tccatcatcc ccgcgcccgt acgttcacga agctccttta ccatggatgc
     2341 agtgatttcc atcagcagac gtccccccgc gtttactcag cgtcctcgaa ttcgtcggca
     2401 tcatccgact ttgcagacgc ttggaattcc ccgttgttgc catccgcgct gaatccctca
     2461 tcttgttctt cctcggcaag agcctgatcc aagccttcac ggccttcgat aacagcatcg
     2521 gcgatcttgg aagtaagaag ccttacagca cgaatcgcat catcgttgcc tgggatcacg
     2581 tagtccacct cgtcaggatc gcagttggta tcgacgatcg caatgatcgg gatatcgagc
     2641 cttctggctt cagccactgc aatcctctcc ttgcgaggat ccacaacaaa cacagcactg
     2701 gggagttgct tcatgtcacg tattccaatc agacgcttct gcaacttagt cctttcggca
     2761 ataagctttt ggacttcctt tttgggcagc gcgtcaaagg aaccgtcctc ttccatctgc
     2821 tcaagcttct tcatccactc cacgcgtttg cgcatggtgg cgaagttagt aagcatcccg
     2881 cctagccagc gctcattcac ccaatacatt ccacaccgct gagcttcttc agcgatcgta
     2941 tcgcgtgcct gcttcttcgt gcccacgaaa agcaccgttc cgccgtcctg tacggtttca
     3001 cggacgaaat cataagcctc gtccagtttt ctcacagtct gctggaggtc gatgatgtag
     3061 atgccatttc gcgaagtgaa gatgtaaggc ttcatcttag gattccagcg ccttgtctgg
     3121 tgtccaaaat gtacgcctgc ttcgagcagt tgcttcatag tcaccactgc catgccaatc
     3181 acctcctccc gcagcggttt cccctccgac ctcatcatcc cggagttcca ccagacgccc
     3241 acctggcgcc cagcaccgcc aaccccgatc ataaggtcgt gtgtagtcgc acagcggtta
     3301 gtatatcaca cgctgaagcg gcctgcaatc tcgccacaag ggcgcatcac acagtgcacg
     3361 agcaacctgt tgccttgcca ttttcgtttg caacagcaac aacgtctccg tttgtcacgt
     3421 aagcagccag gacttgaccc tcccacttac tgcggcccaa acctagcgag tgcgcagcct
     3481 atcaagttcg tcgaataatt tgtcattgag gacccgtatg tatgtgccct tcatcccgag
     3541 cgagcgcgat tcgatcacgc cggcactctc gaacttacgc agcgcattga cgattacaga
     3601 cctagttatg cccactctgt cagcgatttt ggaggccacg agaagccctt catctccggc
     3661 aagttcgtca aagatgtgct cgaccgcctc ctgttctgaa tacgagagcg tttcgagggc
     3721 catctgcaca gctgcgcgct ttctcgcctc ttcctcggcc ttctcacttc ttgacctgta
     3781 gatttccatt cctatgaatg ttgcgccata ttcagcgagc attatgtcgt tttcgccgag
     3841 ttcgccgtcg gacttcgaaa gaagcaacgt tccgactcgc tcacccccgc catatatcgg
     3901 agcgacagtc aagcgacacg attctgacga tccccccaac atctcattgc agaacgcgcg
     3961 catcccatta ctgggcctgc gattcgcggc tgtctcaaat atcgcgagaa actcttcatt
     4021 caccttctgt ggcacgtagc cgttcccaag gatttcggaa tccaatactt caaaatcatc
     4081 gtcagtcagg tggggcagag aatggcccaa tatcttccca tttttcgcaa caacaaaggc
     4141 ctggcctgac acggcctctg caagcgcgtg ggcaatctcc tcaaaacgtc ctgcatgtcc
     4201 tggggaactc tggatcagcc tgcctagctt ccgtgtgcgc tccagtagtg agttcacttt
     4261 gagacctcct ctcaccaacc acggacgcat gtccgtgcgc ttcagattag gagcaagtta
     4321 tgctctttta tcttgtccaa acatttgcgt ttcatcacac acgccatctg ccggcgctac
     4381 agaatgtagt ccgaaagatc gctctcagta accaactgct ccagcctgga ccgcacatag
     4441 tcggcatcta ccacaaccct ctctgaatgc tccggcgcgt tgaaagatac ctcctccagc
     4501 accttttcga gaatcgtatg gagccttcgg gcgcctatat tctcactgct ggaattgacc
     4561 tcgtcggcaa ttctagctat ctcttcaacc ccatcatccg taaacaccaa atcgaccccg
     4621 tcagtagcaa gaagagccat gtattgcttg gtcagagcgt tcttcggctc agtcagtatt
     4681 ctcacaaaat catctttggt gaggcttgaa agctccaccc ttattggaaa tctcccttgc
     4741 agctccggaa tgagatcaga aggtttcgac atgtgaaaag cgccagccgc aataaacaac
     4801 atgtgccctg tcttcacagg cccgtatttc gtctggacag tcgacccttc cactataggc
     4861 aggatatctc tctgaacgcc ttcacgggac acatcgggcc ctgcgccctg ccctttgacg
     4921 gcgatcttat cgatctcgtc gatgaatact atgccgctct gttccgcacg ggcgatagcc
     4981 tcttcagcaa cctcatccat gtcaatgagc ttcgcggcct cctcggtaat gaggattttt
     5041 cgagcttcag aaacaggcaa ttttctgcgg cggcggcgcg gaggcaacat ctcgcccatg
     5101 aggtcctgaa ggtttatccc catctcttcc actccggccc cggaaaagat ctctatcatc
     5161 ccgccctgta cgtcatcgat ctcgatctca atgatcacat cttccagttc gccccttctc
     5221 aggcgttccc tcgtctcttc cctgcgcgcc cgtgcgctgg ccagtttcga ctcaaacatc
     5281 cggtcatcct gcggctgccc gacaggcatg ccccagaggg cggcaaacgg gttggcctga
     5341 gaacgatcgc gagtcggcaa cggcgctaag gcatccagca gacgctcctc gcccagtatt
     5401 ttcgcttgct cctgcacgcc cgacatcttc tctgctttta ccatcctcac tgaagtctct
     5461 acgagctccc gcacaatgct gtcgacgtcg cgtccaacat atccgacttc agtaaacttc
     5521 gtcgcctcca ccttcacgaa aggagcgttg acgagccgag ccaagcgtct ggctatttcg
     5581 gttttcccta ctccagtggg gccgatcatc agtatattct tgggtattat gtcgtcctgc
     5641 atttccggag ccaacgccag cctgcgttgc cggtttcgca gcgcaatggc cacggccttc
     5701 ttagcggagg cctgccctac tatgtatttg tcgagttcgc gcacgatcgc gcgaggagtt
     5761 aggtcctgca ttacaacgcc tcccgactac aattcctcta tgttgatctt tccgttcgtg
     5821 tatatgcaaa tctcggatgc gatctccagc gacttgcgga ctatctccac agcctcaagg
     5881 tctgtattcc tgagaagagc ccttcccgca gccaaggcat atgggcctcc ggagcccacg
     5941 gcggtgatgg cgtcatccgg ttccaggacc tcgccatttc ctgaaagtag cagtatcctg
     6001 tttccgtcag tgactatcat catagcctcc aaccttctca agacccggtc tgtgcgccac
     6061 tcccggccaa ggtcgctcgc tgcccgaaca aggttaccct tgtattcaga gagcttgcct
     6121 tcaaagcgct caagaagcgc catagagtca gaagccgaac cggcgaaacc agccagcaca
     6181 ttccagtcag acagtttccg gatcttccgg gccccgtgtt tgacgacggt atcccccaag
     6241 gttacctggc cgtcggaacc catggcgcac ttcccatcac gaatgacggc tacaacggtc
     6301 gtgccagtca tccttattga atctgattca ttcattctgt ctgaagctt
//
LOCUS       DAOSAI010000097         6334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7834_length_6334_cov_3.443383, whole genome shotgun sequence.
ACCESSION   DAOSAI010000097 DAOSAI010000000
VERSION     DAOSAI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7834_length_6334_cov_3.443383"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1004
                     /locus_tag="PLM83_04615"
     CDS             168..1004
                     /locus_tag="PLM83_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOK70723.1"
                     /translation="MSSLNALLSMGAMGLIFGISLGAASKKLAVEQDPRIDQIMEVLP
                     GANCGGCGFAGCSALAGAIVSGDAPVTGCPVGKSAVAEKIAKIMGVECATTEPKHARV
                     LCRGSRKNAAELYEYRGLQDCRSAQLTGGGAKVCTYGCLGFGSCVAACQFGAMSMGPD
                     GLPVVDDSLCTGCGKCVGVCPRGLIELRAAGESVDVVCKSHARGPVVRQACSVGCIGC
                     GLCARNCPEKAIEIVNNLAVIDQAKCTRCGICIEKCPQKCIVDSAGVEGITPAKKDEA
                     AV"
     gene            1289..1864
                     /locus_tag="PLM83_04620"
     CDS             1289..1864
                     /locus_tag="PLM83_04620"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOK70724.1"
                     /translation="MSRLILASASPRRSQLLHGIGLDFEVIPSNVVEEEVNGADPADL
                     VMRLAYAKAVDVAGGIDHGLVIGADTIVVADGHVLGKPVDRDDAIRMLHKLSGKEHQV
                     YSGVSVIDAGSGNSATEYEMTNVKFRTVSDEKIARYVDTGEPMGKAGAYAIQGIGGLL
                     VESIRGDYNCVVGLPLGRLALMLESFGYVVF"
     gene            1917..2636
                     /gene="radC"
                     /locus_tag="PLM83_04625"
     CDS             1917..2636
                     /gene="radC"
                     /locus_tag="PLM83_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOK70725.1"
                     /translation="MKQVDPGRCGMGLSIREMPPSERPMERLLELGPEALSTAELIAV
                     IIRCGRPGESAVDVASRVLSECGTARKLATAGVCELSSIPGIGPVRAAQIVAAIELGR
                     RVVVSEGEPRLSISSAKDVVDLLMPSMRYLEKEEFRTIFLDTRNRVIESATISIGTLN
                     SSIVHPREVFRAAIRAGSAGVILAHNHPSGDPAPSPEDIAITKRLVRAGALIGIEVLD
                     HVIIGDNLYISFKEKGYMNFG"
     gene            2671..3711
                     /locus_tag="PLM83_04630"
     CDS             2671..3711
                     /locus_tag="PLM83_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOK70726.1"
                     /translation="MACASLFGGLARDMGIDLGTANTLVYVRSKGIVLQEPSVIAIDR
                     DSQEILAVGYDAKKMVGRTPASIVAVRPMRDGVIADLDVTVTMLRHFIEKASKRHGVF
                     KPRVVIGVPSGVTQVERRVIHDSAIQAGAKEAFLIEEPMAAAIGAGLPVEDATGNMVV
                     DIGGGTTEVAVISLGGIVAAKSITTAGDKMDEAIVNYMKRNYNLMIGERTAEEIKKTV
                     GSAYPSQQEETMEARGRDIVNGLPKTVTISNTEIQQALADPVGEIVNAVRMTLEKTPP
                     ELSADVIDKGIVMTGGGSMLQGLDRLLCDVTGMPVYLTEDPLTCVARGAGNVVEHLTT
                     YRRVLVSSGRLK"
     gene            3733..4584
                     /gene="mreC"
                     /locus_tag="PLM83_04635"
     CDS             3733..4584
                     /gene="mreC"
                     /locus_tag="PLM83_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOK70727.1"
                     /translation="MNQQPLSKRRIVVAAICIILLVCLIYFTAKDRDRLSVAEKAVRE
                     IFSPIARGASKVSREISSWASYLISIGETKAENEKLREELDALRYENLQMEEALRENE
                     RLRDLLEFKESLGGRAVAARISYRDPGNWLYSVAIDKGSDHGISSGKPVVTARGVVGR
                     VTSVTRNTSTVILTVDSRSAVGGIDFRSRDLVLVEGLGDGSGLLHVRPLGADNDVQPG
                     DIIITSGLGGIYPKGYVLGDVISVAPGKYGVSKSAFARPRVDFDRLEEVLVLIDPIRG
                     AIGEMQE"
     gene            4581..5096
                     /gene="mreD"
                     /locus_tag="PLM83_04640"
     CDS             4581..5096
                     /gene="mreD"
                     /locus_tag="PLM83_04640"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOK70728.1"
                     /translation="MKRLNRLHIALGMFVALLLQAAVFPVLFPRWVVPDLVFCFSAVM
                     SIIEGTGPGVVLAAIGGLCQDALSGTFMGASAGANMAAAWIIGLVEPQLFKENLITPV
                     VIVGAGTVIREVIYTFVIWSFGAGIDWARAFGTVIPWVAVLNCCAGALLHYWIYLRPR
                     DRERLRSVSSV"
     gene            5130..>6334
                     /locus_tag="PLM83_04645"
     CDS             5130..>6334
                     /locus_tag="PLM83_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOK70729.1"
                     /translation="MMNRTRTQSERRLRVFQIFALCLFLVLVGRLWQLQIIKGEYYKS
                     RATANRLALVPISAPRGIITDRRGEVFATSRMAYTISVVPQEFSDREAEVRLLSKLLE
                     MPVEEIEAKIAGQTEGRYGLPYMPTRLIEDVPPYMLVRVSEHKVELPGVIIEEEPYRS
                     YPNGSLAGPIIGYVGLINQEELARLGEKGYQSRDRIGKSGVEREFEEYLRGQDGVTEI
                     EVDSLSRPVATVGARAPVAGSTLVMTIDAKVQQSAEDALRSQLETLQSGKYKHATAGA
                     AVALDPRTGEIIAMATEPGYDPGWFVPRISDEQWRQVSTGVSGLFNRAISGAYPPGST
                     FKPFTAIAALEAGVIDLDDRILCSPSVSSRYYRKRCSIWSRGRSHGYQNLTEGMINSC
                     NIVFYELGA"
BASE COUNT         1378 a         1457 c         2018 g         1481 t
ORIGIN      
        1 tggcgccgat accccgggcc atggagggat tccccatagc ccttgtaact actgggctgc
       61 tcgccatggc ttttctgggc ttttcggggt ttgatgttgc taggttcatg gggttgtagg
      121 cgcgacgttt cgactcatgg tttcgctcgt agatgggggt gacatagatg agcagtctta
      181 atgcgttgtt gagcatgggg gctatggggc tgatcttcgg gatttcgctc ggggctgcat
      241 cgaagaagct agcagtagag caggatccga ggatcgatca gataatggaa gtgctgcctg
      301 gcgcaaactg cgggggatgc ggatttgcgg ggtgttctgc gctcgcgggc gctattgtgt
      361 cgggcgatgc gcctgtaaca ggttgtcctg ttggaaagag cgcggtggca gagaagattg
      421 caaagataat gggagtagag tgcgcaacca ctgagcccaa gcatgcaaga gtcttgtgtc
      481 gcgggagtcg caaaaatgca gcggagctct atgaatacag gggacttcag gattgccgat
      541 ccgcccagct cacaggggga ggcgccaagg tatgcaccta tggctgtctc ggctttggct
      601 cgtgtgttgc agcatgccag tttggagcga tgtcgatggg gcctgacggg cttcccgtag
      661 tcgatgattc gctctgcact ggatgcggaa agtgcgtggg tgtgtgcccg cgagggctga
      721 tcgagcttcg tgctgcgggg gagtctgtgg acgtggtgtg caaatctcac gcgcgcggcc
      781 ccgtggtgcg gcaggcgtgt tctgtggggt gcatcgggtg tggactatgt gccaggaatt
      841 gtcctgagaa ggccatagag atcgtgaaca atctcgccgt tatcgaccag gccaaatgca
      901 ccagatgtgg aatttgcatc gagaaatgtc ctcagaagtg cattgtggat tctgctggtg
      961 tcgaaggaat tacgcccgca aagaaagatg aggccgcagt ataggcgatc gctgtttgtg
     1021 tttgccagga ccagcctctg tgctcaagta cggattgatc agtgttttga ttgacgcggc
     1081 ctcggtcaaa ggcttgtcca tgggcggcgc aaaagttgtg gacgggcttc catctatggg
     1141 agcccgttct cgtcattcca ggtcttgatc gatgcgacat tcttgtaaac gtagaattca
     1201 aatggtagac ttaataaagg taaagagggc cctttgggcg gcgccgcctt tggcatcgct
     1261 cttgtcgggc gcggggagtt ttgatgtcgt gagtaggctg attttggcat ctgcttcgcc
     1321 aagacgttct cagcttttgc acgggattgg gttagatttc gaggtcatcc cgagcaacgt
     1381 tgtggaggaa gaagtgaacg gtgccgaccc tgcagatctc gttatgcgac ttgcgtatgc
     1441 caaggctgtt gacgtggcag gcgggatcga tcatgggctc gtgatcggtg ccgataccat
     1501 agtcgtcgcg gacgggcatg tccttggaaa gccggtggac cgggatgatg ctatccgcat
     1561 gttgcacaaa ctctcaggga aggaacacca ggtatactcg ggcgtttcgg tgatagatgc
     1621 cggaagcggc aactcggcaa ctgagtatga gatgacaaat gtcaagttta ggactgtttc
     1681 ggatgagaaa atagcaagat atgtcgatac cggcgaaccc atgggcaagg ctggcgctta
     1741 tgccatccag ggaattggag gtttgctcgt ggaatcgatc aggggcgact acaactgcgt
     1801 agttgggctt ccattgggcc ggcttgcgct gatgcttgag agttttggtt atgtagtttt
     1861 ttagttgcgc ctcgccatgg caacgcttgc acacaaaatg caggtgaggt gcgataatga
     1921 aacaggttga ccctggacgg tgcgggatgg gcctcagcat cagggagatg cctcccagcg
     1981 aaaggccgat ggaaaggttg ctcgagcttg ggccggaggc cctttccact gcggagctga
     2041 tagctgtgat aatcaggtgc gggcgcccgg gggaaagcgc agtcgatgtt gcttcgcggg
     2101 ttctctcaga atgcggtacg gcgagaaaac tagcgacagc aggcgtatgc gagctatcga
     2161 gcataccagg aataggaccc gttcgagcgg ctcagatagt tgcagccatc gaacttggca
     2221 gaagggttgt agtgtcggaa ggggagccaa ggctttctat atccagcgcg aaagatgtag
     2281 tggacttgct catgccgtca atgcgttatc tcgaaaaaga ggagtttcgc accatcttcc
     2341 tcgatacgag aaatcgtgtg attgaaagtg cgaccatatc cataggcacg ctgaactctt
     2401 ccatagttca tccgcgcgag gttttccgag cagctattcg cgcaggcagt gcaggtgtga
     2461 tcctggccca taatcatcca agcggtgatc ctgccccgag ccctgaggat attgcaatta
     2521 caaagaggtt ggtgcgcgct ggcgctttga tcgggataga agtgctggat cacgtgataa
     2581 taggggacaa tctgtacatc agtttcaagg aaaaaggata catgaatttc ggctaattaa
     2641 gaatcttgcc acagaaggga gccattcgtt atggcgtgcg cttcgctctt tggcggcctg
     2701 gcaagggaca tgggcattga ccttggcacc gctaacacac ttgtatatgt aagaagcaaa
     2761 ggcatagtgc ttcaggaacc ttccgtgatc gccatcgacc gcgactctca ggagatcctg
     2821 gccgtgggtt atgacgctaa gaagatggtg ggaagaacgc ctgcaagcat tgttgcggtg
     2881 aggccgatgc gggacggcgt aatagccgat ctcgacgtga ctgtcactat gttgaggcac
     2941 tttatagaaa aggcctcaaa gcggcacggg gtcttcaagc ctcgggtggt aataggggtt
     3001 ccatcaggtg ttacgcaggt tgaacgccga gtaatacatg actcagcaat acaggcggga
     3061 gcgaaggaag cattccttat cgaagagccg atggctgcag ctatcggcgc cgggctccct
     3121 gtggaagacg ccaccggcaa catggttgtg gatatcggtg gcggaaccac ggaggttgca
     3181 gttatatccc taggcggcat tgtagcggcc aagtccatta caactgctgg agacaagatg
     3241 gatgaagcca tcgtgaacta catgaaacgc aactacaatc tgatgatagg ggaacggact
     3301 gcagaggaga taaagaagac tgtggggtcg gcctaccctt cgcagcagga agagactatg
     3361 gaagcgcgcg gacgcgacat cgtcaatggg cttcccaaga ctgtaaccat ttctaacaca
     3421 gagatacagc aagcgctcgc cgatccggtg ggtgagattg tgaatgccgt gcgcatgact
     3481 ttggagaaaa ctccccctga actgtcggcg gacgtgatcg ataagggcat cgtcatgaca
     3541 ggcggcggat cgatgctgca ggggttggat aggctgctct gtgacgtcac gggcatgccc
     3601 gtttacctta ctgaggatcc gctgacatgt gtcgcgcggg gagcaggcaa cgtcgtggag
     3661 caccttacaa cctatagaag ggtccttgta tcgtcaggga gactcaagta agggcccaga
     3721 gggggcgctc ccatgaacca gcaacctttg tcaaaaagaa gaatcgttgt tgcggcaatc
     3781 tgcatcatcc tcttggtgtg cttgatatac ttcactgcaa aggaccgcga caggctctct
     3841 gttgcggaga aggcggtgcg cgaaatattc tcccctatag ctcgaggggc aagtaaggtg
     3901 agcagggaga tctcatcatg ggcttcgtat cttatttcga taggcgagac gaaggccgag
     3961 aatgagaagc ttcgggagga gcttgacgcg ttgcgctacg agaacttgca gatggaggag
     4021 gccttgcgag agaacgaaag gctcagggac ttgctggagt tcaaggagag tctaggggga
     4081 cgcgccgtcg ccgccaggat aagctatcgt gatccaggaa actggcttta ctctgtggca
     4141 atcgacaaag ggtcagacca tggaatttca agcgggaaac ccgtagttac tgctcgcggg
     4201 gttgttggaa gagtcacgtc tgtcacccgg aatacgtcta cagtgattct gactgtggat
     4261 tcccggagtg ctgttggtgg catcgatttc agatcacgtg acctcgtgct ggttgagggg
     4321 ttgggcgacg gttcaggttt gctgcacgtt cgtcctttgg gcgcagacaa tgatgtgcag
     4381 ccaggggaca taatcatcac ctctggcctt ggtggcatat atcctaaagg ctacgtgctc
     4441 ggtgatgtaa tctcggtggc gcccgggaaa tatggtgtgt cgaaatcggc gtttgcgcgg
     4501 cctcgagtcg attttgacag gctcgaagag gtcttggtct tgatcgatcc gataagggga
     4561 gctattgggg agatgcagga atgaaaaggt tgaacagact gcatattgct ttaggcatgt
     4621 tcgttgcatt gctgttgcag gctgcggtct ttcctgtgct tttccccagg tgggtagtgc
     4681 ccgacctggt gttctgtttt tcggcagtaa tgtccatcat agagggaacc gggcctggtg
     4741 ttgttcttgc agctatcggc ggactatgcc aagatgcgct cagtggaacg ttcatgggcg
     4801 caagcgctgg cgcgaacatg gccgcggcat ggattatcgg cttggttgag ccccagcttt
     4861 ttaaggagaa tttgattacg ccagtggtca tcgtgggcgc gggaacggtg atccgtgagg
     4921 tgatttacac attcgttatc tggtcatttg gcgcaggcat cgattgggcc agagcgtttg
     4981 gaacggtgat tccgtgggtt gcggtgctca attgttgcgc gggcgccctg ctccactact
     5041 ggatctacct tcgtccacgg gaccgagagc gtttacgatc ggtttcgtct gtctagactt
     5101 ttggcgtgaa gcttttccgg ggttgatgta tgatgaatcg aacacgaacg cagagtgagc
     5161 gccgactccg ggtattccag atctttgcct tatgtctgtt cctggtcctc gtgggcaggc
     5221 tgtggcaact gcagataata aagggagaat attacaagag cagagctacg gctaataggc
     5281 tcgcacttgt gccgatatct gcgccgcgag ggataatcac tgataggcgc ggggaagttt
     5341 tcgccaccag taggatggca tataccatct ccgttgttcc gcaagagttt tcggatcgag
     5401 aggcggaggt gcggcttctc tctaaacttt tggaaatgcc tgttgaggag atcgaagcga
     5461 agatagccgg gcagactgag ggccggtatg gtctgcctta catgcctacg cgcctcatcg
     5521 aggatgtgcc cccctacatg cttgtaaggg tctccgagca caaagtcgag ttgcccgggg
     5581 ttataatcga ggaagaaccg tatcggagct atcccaatgg atccttggcc gggcccatca
     5641 tcgggtacgt cggtctgatc aatcaggaag aacttgctcg tcttggcgaa aagggctatc
     5701 agtcccgtga ccgtattggc aagagtggtg tggagcggga gtttgaggaa tacctacgtg
     5761 ggcaagatgg agtcactgaa atcgaggttg actcgctttc tcgacctgtt gcgacggtag
     5821 gcgccagggc tccggtggcc ggctctaccc tggtgatgac gatcgatgcc aaagtgcaac
     5881 agtcggctga ggatgctcta cggtcgcagc ttgagacttt gcagtctggc aaatacaaac
     5941 acgctacggc gggcgccgcg gtagcgctgg atccgcgcac aggagagata atagccatgg
     6001 caactgagcc aggctacgac ccgggctggt ttgttcctag aatctccgat gaacagtggc
     6061 gtcaggtgag cactggcgtg tcggggcttt tcaaccgtgc gatatcaggc gcttatccgc
     6121 ccggctccac gtttaagcct ttcactgcca tagctgcgct cgaggcaggg gtgatagatc
     6181 tcgacgacag gattctctgc tccccctctg tctcctcgcg ctactacagg aagaggtgct
     6241 ctatttggtc aaggggccgt tcgcacgggt atcagaatct tactgagggt atgatcaatt
     6301 cctgcaacat tgtcttctac gagctgggag cgag
//
LOCUS       DAOSAI010000098         6301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7890_length_6301_cov_3.640730, whole genome shotgun sequence.
ACCESSION   DAOSAI010000098 DAOSAI010000000
VERSION     DAOSAI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7890_length_6301_cov_3.640730"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..769)
                     /locus_tag="PLM83_04650"
     CDS             complement(188..769)
                     /locus_tag="PLM83_04650"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOK70730.1"
                     /translation="MEQDLTEKAISSETIYNGKIVTLNVDKVELPNGRTATREVVRTR
                     DSVAVVPVTRDDKVVLVRQYRYAGGEELLEIPAGRIDPGESPRAAAQRELLEETGCTC
                     AELKKIAGFYIAVGFATEFMHLYAGTVDHIGEACPDDDEFVRTELMPVSQIPEILAKQ
                     EIRDVKTLAGLLHLSWMLGLSPVSSCACSICRA"
     gene            complement(1101..2162)
                     /locus_tag="PLM83_04655"
     CDS             complement(1101..2162)
                     /locus_tag="PLM83_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HOK70731.1"
                     /translation="MNAQVAEVVDVVSEFPDLQELSVKLAENGCTVRAICYPKITGSA
                     QPGDEVIVNTTAVDLGLGSGGTHFVVWVLGRDRLSNQLPGHIMKMRYTPMQLATGSYE
                     ENPAYHGAIDSFSGLDGMPVVICELHSMVAPVLAGFSGKAAYIMTDGAALPYALSKVV
                     RRLKEERLFHVSITAGHAFGGDLEAVNVHSALVAAYAVCRCEGAVVAMGPGIVGTGTQ
                     YGFSGIEQAHIADAVNRMGGRAIIVPRLSRCDPRERHHVLSHHTVTVLADVCCTPATC
                     VLASDMDDGFRRKVEERLCGILKAGGHRLAYAPGGGAIDELKERGIELSTMGRGFDEE
                     PEFFLTSAAAGAYARKLVH"
     gene            complement(2654..4000)
                     /gene="rimO"
                     /locus_tag="PLM83_04660"
     CDS             complement(2654..4000)
                     /gene="rimO"
                     /locus_tag="PLM83_04660"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOK70732.1"
                     /translation="MSKVGDLGTKIAFCSLGCSKNLVDTEIMMGLLRDAGMEIVSDEH
                     LADVILVNTCSFIADAAQESVDAILDYADLRADGKLQALLVTGCLPQRYGVELMREIP
                     EIDGIIGTGQFGRCVEAVEDAVAGRRPAFLDDPGYLQPADAPRVVTTPAHYAYLKIGE
                     GCANRCAYCIIPSIRGGVQSRPLEAVVGEAEKLVADGMREAIVIAQDTTVYGVDLYGV
                     PSLAKLLRKLARVEGLEWIRVMYAYPSRIDDELVEVMASTPNIVKYIDLPMQHGSDRL
                     LRAMNRHASRDQMIEVVARLREQVPGIVIRTSLIVGFPGETDRDFEELLDFIKIIRPE
                     RAGVFKFSREEGTRAYDMPEQVPDDVAEARRDAAMEALRRISREYGLSRVGSKVRVIV
                     DGPSDESDLVVEARSFAEAPDVDGKIYIGDATLRPGQLVDVIIEDASDYDLAAVLV"
     gene            complement(4025..4810)
                     /locus_tag="PLM83_04665"
     CDS             complement(4025..4810)
                     /locus_tag="PLM83_04665"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK70733.1"
                     /translation="MKDQNRIHRLVLVVCYLAIVCMAMVGLSGADATLARGAIEVADQ
                     LYSRRSEPGNADAIIAGLEQANKATPGDCEILWRLSRAYWFKSTRLPEKDKKARLDLL
                     AQGRAYAQEATEANPRNANAYYWLAASIAQEGQIRGILQSLASVKPTKDALDKGLEID
                     PKHASCHYLMAELYLQLPGPPLSIGNKQKAVEEAKLAVKYDPSESCHHLVLGRALIAT
                     KQYDEARAVLNRLIAMAPDPGDPEGFRKDQDAARAELKSIAGK"
     gene            complement(4849..5118)
                     /locus_tag="PLM83_04670"
     CDS             complement(4849..5118)
                     /locus_tag="PLM83_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOK70734.1"
                     /translation="MVKVALTTFVTVFLAELGDKTQLATMMLVAEGKSPWAVFLGSAS
                     ALVVSSLVGVLAGGFIGKYVPAMWVRIGAGSAFIILGVLLLMGRF"
     gene            complement(5100..6005)
                     /locus_tag="PLM83_04675"
     CDS             complement(5100..6005)
                     /locus_tag="PLM83_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK70735.1"
                     /translation="MIEPAHVKGVDYLLTEAASIDIDSLVDLFNDAYSQYYVSTVVTP
                     ERLIKLVDREDIYMHHSFVALYGSRPVGVVFLGVRGERGYICGMGVRIAYQGRGVGEL
                     LMRRVLKEAETLGLNSLQLEVVDRNFRAIGLYTKLGFVPIRDLGIWERNQMPRRKGAG
                     RWNPKGFTLNHVASFQALPLVMEFNEIRPCWQNEPTGLAKFCSTLSALVARADSEKIA
                     YALYVDTVEGLYIVDFAATPSLDEGTRQKACEVLLEAIDKQMPRPTARAHNIPVHGYQ
                     GRVLRGRGFDIGLKQQEMVLWLKSR"
     gene            complement(6002..>6301)
                     /locus_tag="PLM83_04680"
     CDS             complement(6002..>6301)
                     /locus_tag="PLM83_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70736.1"
                     /translation="APECIPPDRVFGSLDRLASKVIDGSPKPIAGDMFLMALAFMCFD
                     PLGDPDRCYALGSVLARLDAACQDAERQPDVCDWPGLGRLVCDAISLMSAAGDEL"
BASE COUNT         1444 a         1946 c         1470 g         1441 t
ORIGIN      
        1 ccttcgctat attggcaaat gttagattgc gcgcacccga aacttttaca ggcttacctg
       61 cagaatctct tccgatatgg acgtgaaggt cgacaaagta gcggttgagc cctcgttctg
      121 acattatgta aacatcccca ataaaagtca gtattgatcg atcagattat cccattggat
      181 tttggcatta cgcacgacat atgctgcaag cgcatgagct gacaggagaa aggcctaaca
      241 tccacgaaag gtggagcagt ccggcaaggg tttttacatc ccttatctcc tgctttgcca
      301 ggatctcagg tatctggctt accggcatca gctccgttct gacgaattcg tcatcatcgg
      361 ggcaggcctc gccaatgtgg tcaactgttc ccgcatacag gtgcatgaac tccgtggcaa
      421 agccgaccgc aatatagaag cctgcaattt tcttcagttc agcgcatgta caaccggtct
      481 cttcaagcag ttctctttgc gctgccgccc taggggattc tcccggatcg atacggccgg
      541 ccgggatctc aagaagctct tcaccccctg cgtacctgta ctgccgcacg agtacgacct
      601 tgtcatcacg cgtcacaggt accactgcca cggaatcccg cgttcttacg acttccctcg
      661 tggccgtgcg cccgtttgga agttcaacct tgtccacatt aagagtcaca atcttcccgt
      721 tgtagatcgt ctcactgcta atagcttttt cagtaaggtc ttgttccatt gatatctgcc
      781 tcctgaacat ccatgtagtt tccgcaaagc ctctccgtga tttcccggcc tttgctcttc
      841 caatcgccgc tcggcaactc ccccccgcca caccagcatg ctccgcggag catacagacc
      901 cattgtagct acaaaaacat gcaaaaacca ggatgtagac tccgaccatc tcaactgtct
      961 tcttccgtat gccggacata ttcggacaga tgaagcacta tccccaggca actcccggtc
     1021 agccttccac cctatctgtt cattcgaacc atttgcctct agcccaagtt acacgggagc
     1081 gcccgaagac ttttgcaaag ctagtggacg agttttcgtg catacgcgcc tgccgccgca
     1141 gaggtgagga aaaactccgg ctcctcgtcg aacccgcgcc ccattgttga gagttcgatc
     1201 ccccgctcct tcagttcgtc tatggctcct cctccaggcg cataagccaa ccgatgccca
     1261 cctgctttca gtatcccaca gagacgttct tccaccttcc ggcggaagcc gtcatccata
     1321 tcggaggcca acacgcatgt agccggcgtg caacagacgt ccgccaacac ggtgactgta
     1381 tggtgactca atacatgatg gcgttcccta ggatcgcatc ttgaaagccg gggcactatg
     1441 attgcgcgcc cccccattct gttgactgca tcagctatgt gtgcctgctc tatgccggaa
     1501 aagccgtatt gagtgcctgt tccgactatg cccggaccca tcgccaccac cgctccctcg
     1561 cacctgcata cggcataggc agccacaagc gccgaatgga cattgaccgc ctccagatcg
     1621 cccccgaagg cgtgcccagc agtaatcgag acgtggaata gtctttcttc tttcaggcgg
     1681 cgaactacct tgctcaaagc gtatgggagc gctgctccgt ccgtcattat gtaagcagcc
     1741 tttcctgaaa acccggcaag gacgggagcc accatcgaat ggagctcgca tatgaccaca
     1801 ggcatgccgt ctagccccga aaaactgtca attgccccat ggtacgctgg attctcttcg
     1861 tagctccctg tagccagttg catcggagta tacctcatct tcattatgtg accaggcagc
     1921 tgattgctca aacggtccct ccccaacacc cacacaacga agtgagtacc accgcttcca
     1981 agtccgaggt cgaccgcagt ggtgttcacg atcacctcat cgcctggctg tgcactgcct
     2041 gtgattttag ggtaacatat tgcccgaact gtacatccat tttcggccag ctttaccgaa
     2101 agctcttgta gatcaggaaa ctcggaaacg acgtccacga cctcggcaac ctgtgcgttc
     2161 atcaactccc ccacctccac aaagtagctt ccccctagcg gcaaacggtc acacaggaca
     2221 tggtgcacca tgcgacagct gcgcggtggt tagtgcctag catccgcgga tgtatccaat
     2281 cacgaatttg cccccaggga ctggcggcgg aaaaagtctg gggggctgta tttcgtgatg
     2341 ctcggcatgc cgatccaagc gattcctatg ggcacgcaac gtggaagtga agacattgct
     2401 caggcgattt gcttgccctc ggaaatgaca gttggcagca gaagtagccg acctgcagca
     2461 tcatgatgat ttacgtgttt accaaggtca tgccgagaaa ctcgcctacg cctgcagttt
     2521 cagcccgagg ttgcgtactt agagatccag tgtagaacac gaatttccac cacaacatag
     2581 ccgccttccg ggccgatcat cggctcagaa ggcggcgcat tctcacctga tcttctattg
     2641 gcagaccgcg cccttacacc aataccgccg ccaggtcata gtcgcttgcg tcttcaatta
     2701 tcacatcaac aagttgaccc ggccgcaatg ttgcatcgcc tatgtatatc ttgccatcta
     2761 catccggcgc ttccgcgaaa gaccgagctt ccacaacgag gtcagactcg tcagacggcc
     2821 catccacgat cacccgaacc ttactgccca ctctagaaag gccgtattct cgggagattc
     2881 gcctcaaggc ctccattgca gcatccctgc gcgcctcagc cacgtcatcg ggaacctgct
     2941 caggcatgtc gtaggcccga gtgccctctt cacgggagaa cttaaatact ccagctcgct
     3001 caggcctaat tatctttatg aagtccagta gttcctcgaa atctctatct gtctctccgg
     3061 gaaacccgac gatgagcgag gtgcgtatga cgattccggg cacctgctcc ctcaggcgcg
     3121 caaccacttc gatcatctga tcgcgcgagg cgtgtcggtt catggcccga agcaacctat
     3181 ctgaaccatg ctgcatcggc aggtctatgt atttcacgat gtttggcgtt gacgccatga
     3241 cctcgacaag ctcatcgtct attctggaag gatacgcgta catcaccctt atccattcaa
     3301 gcccctctac gcgagcgagc tttcgcaaga gttttgccaa cgatggcacg ccgtacagat
     3361 ccactccata cacggtcgtg tcctgggcta taacgatggc ttcccgcatg ccgtcggcaa
     3421 ctagtttttc cgcctccccc actaccgcct ctagagggcg gctctgcacc ccaccccgaa
     3481 ttgacgggat gatgcagtat gcacacctgt tcgcacaccc ttcgccgatc ttcaggtacg
     3541 cataatgagc aggcgtagtc accactcgag gagcgtccgc cggctgcagg taccctggat
     3601 catcaagaaa agcgggacgt ctgccggcaa cggcatcctc aacagcttct acgcatcgac
     3661 cgaactgtcc ggtgccgatg atgccatcga tctcgggaat ctcccgcatc agttccacgc
     3721 cgtatctctg tggcagacag cctgtgacaa ggagagcctg caacttccca tccgccctta
     3781 gatctgcata atccaagata gcatcgacgg attcctgggc tgcatcagca atgaagctgc
     3841 acgtgtttac gaggataaca tctgcaagat gctcgtctga cacaatctcc attccggcgt
     3901 cgcgaagaag ccccatcatt atttcggtat ctacaagatt ctttgaacat ccaagtgagc
     3961 aaaacgcgat cttcgttcct aagtcaccaa ccttcgacag atcaatgctt ctttgtgcaa
     4021 gagattattt tcctgcaatc gatttcagtt ctgcacgggc cgcatcctga tccttccgaa
     4081 atccctccgg atctcccgga tcgggcgcca tggcgatcag cctgttcaag acggctctgg
     4141 cctcatcata ttgcttcgtc gctatcaggg cccgtccaag aacgagatga tggcaggact
     4201 cgcttgggtc gtatttcact gccaatttcg cctcttcaac agccttctgc ttattgccta
     4261 tggaaagcgg aggcccagga agctgcagat acagctctgc catgaggtaa tggcagcttg
     4321 catgcttcgg atcaatttcc aaccccttat ccaatgcgtc cttcgtaggt tttaccgagg
     4381 caagactttg caaaatcccc cttatctgtc cttcctgcgc aatcgaagct gcaagccagt
     4441 aatacgcgtt cgcattcctc ggattggctt ctgttgcctc ttgggcatag gctcgcccct
     4501 gagctagcaa gtcgagcctt gccttcttgt ccttttcagg aagccgcgta gacttgaacc
     4561 aatacgcccg tgacaaccgc cacagaatct cgcaatctcc cggtgtagcc ttgttggctt
     4621 gttccaatcc cgcaatgatg gcatcagcgt ttccaggctc ggatctcctc gagtagagct
     4681 gatcagcaac ttcaatcgcc ccacgcgcca atgttgcatc agcacctgaa agaccgacca
     4741 tagccataca aacgatcgca aggtaacata cgactaggac aagcctgtga atgcggtttt
     4801 ggtccttcac gtcaacatta caccccctgg tttcgtagta ttgtgagatc agaatcggcc
     4861 catgagaaga aggacgccta gtattatgaa ggcagatccg gcccctatcc tcacccacat
     4921 ggctggaaca tacttgccta tgaatcctcc cgcaagcact ccaacaagcg aggaaaccac
     4981 aagggccgat gctgaaccca gaaaaactgc ccaaggcgac ttgccttcgg caaccaacat
     5041 catggtggcc agctgagtct tgtcgcccaa ctcggccagg aatacagtga caaatgtcgt
     5101 caacgcgact ttaaccacaa caccatctcc tgctgtttaa gccctatgtc gaatcccctt
     5161 ccccgcaata cacgtccttg gtacccgtgg acgggaatat tgtgtgcgcg cgccgtcggc
     5221 cttggcattt gtttgtcaat ggcttccaga agaacttcac atgctttctg ccttgttcct
     5281 tcgtcaagtg aaggcgtcgc ggcgaaatcc actatgtaga gcccctcgac ggtatcgaca
     5341 tacagagcat aggcgatttt ttcggagtct gctcgtgcaa cgagcgctga caatgtgctg
     5401 cagaacttcg ccagacccgt tggctcgttc tgccaacacg gccgtatctc gttaaactcc
     5461 atcacgagtg gcagggcctg aaatgaagcc acatggttta aagtgaaacc ttttgggttc
     5521 catctcccag cgccctttct gcgaggcatt tgatttcttt cccatattcc cagatcccta
     5581 atgggcacga accctagctt ggtgtagagg cctatggctc tgaagttccg atccacaact
     5641 tccagctgta gcgagttgag accgagtgtc tccgcttctt tcaatactcg cctcatgaga
     5701 agttcgccga cgccgcgtcc ctggtaggct atgcggactc ccattccgca gatatagccc
     5761 cgttctcccc gtactccaag aaacacgact ccaacgggac gggatccgta aagagccaca
     5821 aatgagtggt gcatgtatat gtcctcacgg tcaaccaact tgatcaggcg ctcaggcgtg
     5881 accacagtcg aaacgtaata ctggctgtag gcatcattga acaggtcaac aagggagtcg
     5941 atgtcaatgc ttgccgcctc agtcaacaag tagtccacgc ccttcacatg cgctggctca
     6001 atcacaattc atcacctgcc gcgctcataa ggcttatggc atcacacact aggcggccca
     6061 gcccgggcca atcacacaca tcaggctgcc tctcagcatc ctgacaggca gcgtcgaggc
     6121 gcgcaaggac actgccgagc gcatagcaac gatcgggatc acccaaggga tcgaaacaca
     6181 tgaaggccaa agccatcaga aacatgtccc cggcgatagg ctttgggctg ccatcgatca
     6241 ccttcgaagc aagacgatcc aagctcccaa acacgcgatc aggagggatg cactcaggcg
     6301 c
//
LOCUS       DAOSAI010000099         6299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7896_length_6299_cov_3.310858, whole genome shotgun sequence.
ACCESSION   DAOSAI010000099 DAOSAI010000000
VERSION     DAOSAI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7896_length_6299_cov_3.310858"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PLM83_04685"
     CDS             complement(<1..960)
                     /locus_tag="PLM83_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOK70737.1"
                     /translation="MANFRIPDVRRLPAIASIFVILALIGSVCARPLSAAADSAITVT
                     FIDVGQGDAILVSTDDGRHMLVDGGPKAAGGKLVATLHSMGVGHLDVVVSTHPHEDHV
                     GGLIEVLDSFAVGKVLDSAKAHTSATYEAYLSKIYEKGIAFSAPRPGDSFDLGPVTIK
                     VLWPTEPLPQDINDSSLVLEVAYGESRILLTGDISGHVERILADTGVLNHIHLLKVAH
                     HGSRTSTTQGFLTQTSPRLAVIQVGQGNDYGHPSNQVLTNLRSRDVLAYRTDEHGSVS
                     VKMRPDGKTFSVHIEQPGNDLRASSSSDVFHRSSCRYVNTIDAS"
     gene            complement(1066..1467)
                     /locus_tag="PLM83_04690"
     CDS             complement(1066..1467)
                     /locus_tag="PLM83_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOK70738.1"
                     /translation="MKGFAIDTFEKNQVVDITAQVESCLADQGPQDGICLVQTPHTTC
                     GIILNEHFDSTVSEDILDCLNRIAPEDADYSHKEGNATAHIKAALIGSSVILAIEDRR
                     LALGTWQRILLCEFDGPRQRKVWVKTLKQGT"
     gene            complement(1756..3327)
                     /locus_tag="PLM83_04695"
     CDS             complement(1756..3327)
                     /locus_tag="PLM83_04695"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOK70739.1"
                     /translation="MRRKQVNILGFVLSENAGVAVVGIKTGLIMPTDDIVEVTMDAVE
                     DILEDGDIVCVTEAVVARSQNRYITCDELAEDVKAKLNIKDNGTVAVISPIVSRNRFA
                     LVLQAIARAVNKGRVIVQLTVPCDEVGNQVIDEEFANNRLRFKKVLRSLQEVRGNTPQ
                     MNVLIREIIAALKFQELGYSVNAIRKITGTGMADITLLDKDGRKVIAEVAFENLAGVA
                     QKAIGIAKDDKADIAMAVAVDLQAQEMAIVEATSFLEGKAKPEVYKYDERQAFYEATD
                     VITVAEIGERLFPHPITGIDYGRMYAATIESEGAQAEIVFTNNPLAVFNFGHIDGIII
                     GAVHERESLKNLFLSFGTKTPMLTIQDIGPEPWGVIGSNVSDLDKGILKLLPDNSDDV
                     CDKIKAAAWEKTGKDIEVLIFGDGAYKDPDTGIYELADPYPSIGCSKGLRSASLRQGT
                     KLKLLVETMFREGHSREEIYKALEEQQPSRETLGTTPRNITGILATMADLAAGSADAG
                     TPVVIIRNFPFKPAK"
     gene            complement(3669..5678)
                     /locus_tag="PLM83_04700"
     CDS             complement(3669..5678)
                     /locus_tag="PLM83_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOK70740.1"
                     /translation="MASEYIPHVVAKAWEDLVNTGTVRVEALRPVVASSWTRSQQAGI
                     DPRDAVGKIVLSQAQLENVILDRSDLIEVARPFMSSLYEFVAGSGFVVIISDELGRVM
                     EVMGDENALRRGALVNLLPGSSWAEHHVGTNGIGTALAIAKPVQVSGEEHYCEKFHPW
                     TCSAAPIRNSNGSIIGALQMSGPSSKTHIHTLGMVVAAIEAIEAQMKINAKVRELTVL
                     NDNLNKMFQIASDGIVLVNNEGIIQRTNPVADRILGTTRPTSFMEATGAPSHLEKMIS
                     NGTAFDDVEMSVETPEGRVQCLVSGKAIYDEDGEIRGGVVSLNPTKRIAKLMGRYSRS
                     YATFTFDDIIGRDGNLRRAIKLGHIAAESDSNVLICGESGTGKEMFAQAIHNEGRQAN
                     GPFVPINCGAIPRELVGSELFGYEEGAFTGAARGGRPGKFELASGGTLFLDEIGDMPL
                     EQQIALHRVLQDKMVTRIGGANMTEVDVRIICATNKNLQHEVAKGNFRQDLYYRLNVI
                     YILLPPLRERREDIPLLFDFFLSKAMSQLGVDISNVDPDVTGCLTSYDWPGNVRELQN
                     VVERMVNIATSGYIGLEHIPVEILNPSRSGNTSTQMGSPFPAAKPPTFEQQRAEIRRT
                     AEQHQREELIRALEKHDGNITRTAEEMGVSRNTIYRRMKKLDLQR"
     gene            5991..>6299
                     /locus_tag="PLM83_04705"
     CDS             5991..>6299
                     /locus_tag="PLM83_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOK70741.1"
                     /translation="MEKPMDRNYKLFIDGKWVDGKEGKTFDTYCPANGELLSTCVEAG
                     PEDVDLAVKAAWKAYDSWKLTSPAQRSQILLEIADRIDANTEKLAMIETLDNGKPIRE
                     S"
BASE COUNT         1283 a         1659 c         1709 g         1648 t
ORIGIN      
        1 ggatgcatcg atcgtattca cgtatcggca tgaacttcgg tgaaacacat cgctggaaga
       61 tgacgctcgc aggtcattgc cgggctgttc aatatggacg gagaatgttt ttccatccgg
      121 cctcatcttg acgctcacag acccatgctc gtctgttctg tatgcgagga catcgcgtga
      181 tctcaggttt gtaagcacct ggtttgatgg gtgcccatag tcgttgccct gccccacctg
      241 tatcactgca agccttggag acgtctgcgt cagaaagcct tgggttgtgg acgttctgct
      301 gccatggtga gcaactttga gcagatggat gtggtttagg actcctgtgt cagccaatat
      361 ccgctccacg tgcccgctga tgtcgcctgt gagcaggatg cgggattctc cgtaagcgac
      421 ttccaggact aaggaggaat cgtttatgtc ttgcggaagg ggctcggtgg gccacagaac
      481 cttgattgta acgggtccta ggtcaaatga atctcccggc cttggagccg agaaagctat
      541 gcctttctcg tatatcttgc tgaggtaggc ttcgtaggtg gctgacgtgt gcgctttggc
      601 ggagtccagt actttgccaa ctgcgaaaga gtccagcact tctatgagtc ctcccacatg
      661 atcctcatgg ggatgcgtgg atacgactac atcgaggtgc ccgactccca tggaatggag
      721 cgtcgctacc agcttgcctc cggccgcctt gggtccgccg tcaaccaaca tgtggcgtcc
      781 atcgtctgtg gatacaagga tcgcgtcgcc ctggccgacg tcgatgaagg tcacggttat
      841 tgcactgtcg gctgcggcag acagaggtcg agcgcagaca gatccgatta gggccaagat
      901 aacgaagata gacgcgattg ctggcaatcg tctgacatct ggaattcgga aattagccat
      961 gggcaccctc cgttcagata cggtcatgat gtggttaatt gggatagagg ttcccgagag
     1021 tcccggatgc gggcagtctc acaggtggtg atccttctta gactctcatg tgccttgttt
     1081 cagtgtcttg acccacactt tccgctggcg cggcccatcg aactcacata gaaggatgcg
     1141 ctgccatgtt cctagggcaa ggcgtctatc ctctatcgcg aggatgactg acgagccgat
     1201 cagagcggct ttgatatggg cagttgcgtt gccttcctta tgtgagtaat ctgcgtcctc
     1261 gggggcaatg cggttgagac agtcgagtat gtcctcggag acagtagaat cgaagtgttc
     1321 gttgaggata attccacatg tggtgtgtgg cgtctgaact agacagatgc cgtcctgtgg
     1381 cccttggtcg gcaaggcacg attcgacctg ggcggtgata tcgaccactt ggttcttctc
     1441 gaacgtgtcg atagcaaatc ccttcacgaa gtattcactc ccctttgtaa ttgccctcgc
     1501 tttcctaaga agataataac atgaacgcga agttttgcgc ttgatggttg tataggctgc
     1561 gtctatagcg aattcggaag atgacgggaa gctcttgctt gatgcggtgt tctttgcacg
     1621 tctgggtggt gaatagactc ctcataggct cactcccaac gacatcaggc atgccgtgga
     1681 gatgtggcga ttgttgtgtg aatgctcgca aaacctagca gggcggcccg acgccgccct
     1741 gctaggtttc aatgcctact tggcaggctt aaaaggaaag tttcgtatga ttaccaccgg
     1801 cgttccggca tcggcagagc cggcggcaag atcagccatg gttgcgagta tcccggtgat
     1861 gttgcgcggt gttgtgccaa gggtttcgcg ggacggttgc tgttcctcga gggccttgta
     1921 tatctcctcg cgggaatggc cttctctgaa catcgtttcg acaaggagtt tgagtttcgt
     1981 gccctgccgc agtgaggcac tgcgaagccc cttgctgcat ccgatggacg gatacgggtc
     2041 agccagttcg tagatgcctg tatccgggtc cttgtaggct ccatctccaa agatcagaac
     2101 ttcaatatcc ttgccagtct tctcccaagc cgcagccttg atcttgtcgc acacgtcatc
     2161 ggaattatca ggtaggagct ttagtatccc cttgtcgagg tctgacacat tcgatccgat
     2221 tacgccccat ggctccggcc caatatcctg tatcgtgagc atgggagtct tcgttccgaa
     2281 cgagagaaag aggtttttca gagactcgcg ttcgtggact gcgcctatga tgatcccgtc
     2341 tatgtggccg aagttgaaga cggccaaggg attgttggtg aaaacgatct ctgcttgagc
     2401 tccttctgat tctatcgtcg cagcatacat tctgccatag tctatcccgg tgatgggatg
     2461 ggggaagagt ctctccccga tctccgcaac agtaatcaca tcggtcgctt cgtagaacgc
     2521 ctggcgctcg tcatacttat atacctcagg ctttgctttg ccctccaaaa atgacgtggc
     2581 ttcgacaatg gccatctcct gagcctggag gtccactgcg accgccatgg cgatgtccgc
     2641 cttatcatct ttggctattc ctatagcctt ctgggcgacg cctgcgaggt tctcgaaagc
     2701 gacctcggcg atgacttttc ggccgtcctt gtcgagcaag gttatgtcgg ccatgcccgt
     2761 tccggtgatc tttcgaatgg cgttcaccga ataaccgagc tcctggaact tgagcgccgc
     2821 gatgatctct cttatgagaa cattcatctg tggggtgttt ccccggacct cttggaggct
     2881 gcgcagcacc ttcttgaagc gaagacggtt attggcgaat tcctcgtcta tgacttggtt
     2941 ccccacttcg tcgcacggga ctgtcagctg tactatgact cttcctttgt tcaccgcccg
     3001 cgcgatggcc tgaagaacca atgcgaaacg attgcggctt acgatgggac ttatgacggc
     3061 gacagtgccg ttatctttta tgttaagttt ggccttcacg tcttctgcaa gctcatcgca
     3121 ggttatgtag cgattctgag atctggccac aaccgcctcg gtgacgcata cgatatcgcc
     3181 gtcctccaga atatcctcga ctgcatccat ggtgacttcg actatgtcat cggtaggcat
     3241 tatcagccct gttttgattc ctacgacggc aacgccagca ttctccgata gaacgaagcc
     3301 caaaatgttc acctgctttc tccgcacatc ttcacctgcc catacgtggt ccagtgagaa
     3361 tatgcggact ttcgtttctg gtccatccaa atcggttccc aaaggcctgc ccagtttttg
     3421 cagcggacgg gcttggggcc gagaagggct actgccagac ttcggcccgt tagaggcttt
     3481 ggcagcaata tttattatcc catgacgttt gtgggattac aagcggaatc tagtcttcgc
     3541 gacggtgcgt agatgctatc ttgatcagct gtgctctacg gcacctgcat cgggttgcgt
     3601 ccaaggaccg ggcgaaacat ggacaatgcc attggtatga cgcggtcgac atggggttag
     3661 gaaacaactc acctttgcag atcgagtttt ttcattcttc tataaattgt gttcctcgag
     3721 acgcccattt cttctgctgt ccgtgttatg ttgccatcat gtttttcgag ggctcgaatc
     3781 agttcttctc gctggtgctg ctcggcagtg cgtctaatct ccgcccgctg ttgctcgaaa
     3841 gttggaggct ttgccgcagg aaaaggtgaa cccatttgcg tcgatgtgtt tccggagcgg
     3901 ctagggttga gtatttctac aggaatgtgt tcgagaccga tgtatccgga tgtggcgata
     3961 ttcaccatgc gttccaccac gttttgaagc tcccttacgt tgcctggcca atcgtatgag
     4021 gtcaaacagc ctgttacatc gggatcgaca tttgatatgt cgactcctaa ttgtgacatg
     4081 gctttcgaaa ggaagaaatc gaaaagcagg ggtatgtctt ctcggcgttc tctcaacggc
     4141 ggcagaagaa tgtatatcac gttcagcctg tagtagaggt cttgtctgaa gttgcctttg
     4201 gcgacttcat gttggaggtt tttgttggtt gcgcagatta tccttacatc tacttcagtc
     4261 atgttcgcac cgcctatgcg ggttaccatc ttgtcctgaa ggactctgtg aagcgcaatt
     4321 tgctgttcaa ggggcatatc gccgatttcg tccaggaaga gagtgccgcc tgacgcaagt
     4381 tcgaatttgc ctggccgtcc gccccgggct gcgcctgtga aggcgccttc ctcgtaaccg
     4441 aatagctcgc tgcctaccag ctcgcgcgga atggcaccac agtttatggg cacaaatggg
     4501 ccattcgcct gtctgccctc gttgtgaatg gcctgggcaa acatctcctt gcccgtcccg
     4561 ctttcgccgc atatcagcac attgctgtcg ctttccgctg ctatatgtcc caactttata
     4621 gcgcggcgga gattgccgtc tctgcctata atgtcgtcga aggtgaaggt ggcgtacgaa
     4681 cggctgtacc ttcccatgag cttggcaatc ctcttggttg gattgaggga tacgacgccc
     4741 cctctgatct cgccgtcttc atcgtagatg gcttttccgc tcaccaaaca ttgcacgcgc
     4801 ccttctgggg tctccacgct catttccaca tcatcgaagg cggtgccgtt tgagatcatc
     4861 ttctccaaat ggctcggtgc gcctgttgcc tccatgaacg aggttgggcg ggtagtgccg
     4921 agaatccgat ctgctaccgg attggttcgc tggattatgc cttcattgtt gacaagcact
     4981 atcccatctg aggctatctg aaacatcttg ttcaggttgt catttaggac tgtgagctcc
     5041 cggactttgg cgttgatctt catctgtgct tcaattgctt caatcgccgc cactaccatt
     5101 cccaaagtgt ggatgtgggt tttcgatgaa gggccggaca tctggagtgc gccaatgatc
     5161 gatccgttgc tgttgcgtat cggtgctgca gagcatgtcc atggatggaa cttctcgcaa
     5221 tagtgctctt ctcctgatac ctgaactggc ttggcgatgg caagggctgt gcctattcca
     5281 ttggttccca cgtgatgttc tgcccagctc gagccgggca gaaggttgac tagcgctcct
     5341 cggcgaaggg cgttttcgtc tcccatgacc tccatcacgc gcccgagttc atcggagatg
     5401 atcacgacga agcctgaacc tgcaacgaac tcgtaaaggc tggacatgaa cggtctggca
     5461 acttcaatca gatcggagcg gtccaatatg acattttcca gctgggcctg gctgaggaca
     5521 atcttgccta ctgcatccct cgggtctatc ccagcctgtt gggacctggt ccaggaggag
     5581 gccacaacgg gcctgagtgc ttcaactctc actgtcccgg tattgaccag atcctcccac
     5641 gctttcgcca cgacgtgggg aatgtactcg ctggccatta gggtgtcccc ctttctgtct
     5701 ttgcgtctcc gcatgcgccc gccaggcggg cgctacacaa gaggcggcaa gatgcatgtc
     5761 atggctctca ttcctattgt tgcatatatg tcaccacttt gtcacgtata ccaccattta
     5821 agttacagct atgtcacatc ttctcaacaa cgtgcctttt ctgagcaggt aaaacctgat
     5881 ggcatgaaag ttgcattgtt tattagtcgc atgatgacaa ccggtgccca tctgaagaat
     5941 ggttcttggg gcgccgtggg gtcaaaacaa gaaggagggg acgatagacc atggagaagc
     6001 cgatggacag gaattacaag ctgtttatcg acgggaagtg ggttgacggc aaggaaggga
     6061 agacctttga cacctattgc ccggccaatg gagagctcct ttcaacctgt gtggaagcag
     6121 ggccagagga cgtggatctt gctgtcaagg cagcctggaa agcgtacgat tcatggaaat
     6181 tgaccagtcc cgcacagcgt tcgcagattc ttcttgagat tgcagaccgc atcgatgcaa
     6241 acaccgaaaa actggccatg atcgaaacgt tagacaacgg caagccgatt cgcgagagc
//
LOCUS       DAOSAI010000100         6294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7903_length_6294_cov_3.668056, whole genome shotgun sequence.
ACCESSION   DAOSAI010000100 DAOSAI010000000
VERSION     DAOSAI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7903_length_6294_cov_3.668056"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PLM83_04710"
     CDS             complement(<1..766)
                     /locus_tag="PLM83_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOK70742.1"
                     /translation="MMLTDNQKRVMGTLARKRVTVVGIGITNVPLIRFLAQAGAKVTA
                     CDIKTADQLGSELASLSGLGIDVSLGPGYLDSLADCDIVFLSPGVNPEQPQIMAARDV
                     GVSFSSEIELVLELSAAPVIGITGSSGKTTTTTLTGRILAEDARLHHPERDVLVGGNI
                     GTPLIEHVLSVESEDLVVLELSSFQLRLLAVSPHIAAVLNITPNHLDVHPNMEDYIFA
                     KENIVRFQSEDEWAVFGEENEITARMATRARGNVLMF"
     gene            complement(879..2024)
                     /locus_tag="PLM83_04715"
     CDS             complement(879..2024)
                     /locus_tag="PLM83_04715"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOK70743.1"
                     /translation="MKVVIAPDSFKGSLAATEVCRAAEEGLRRVWPDAEVVRVPMADG
                     GEGTVEALVDATGGQVIDVEATGPLGDKVQAFFGILGDGTTAVIEMAAASGLPLVPLD
                     KRNPMTATTRGTGELIAAALDRGCRKFIIGIGGSATNDGGAGMAQALGARLLDAEGKE
                     IGPGGAELARLTRIDTSGMDRRLVGAEFVVASDVDNPLTGPNGASAVYGPQKGATPEM
                     VQVLNAALANYASVIRRDLRIDVEAMPGAGAAGGLGGGLVAFLGARLEPGVEIVIETT
                     GLKEKVSGADLVITGEGRIDFQTLYGKTPMGVARVATECGVPVAVLAGSVADDARGLH
                     DHGIDALLTVVKGPCDLDAAVRRAYANVADAAETLARLIEIGRRQRG"
     gene            complement(2154..3455)
                     /locus_tag="PLM83_04720"
     CDS             complement(2154..3455)
                     /locus_tag="PLM83_04720"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:NF009814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOK70744.1"
                     /translation="MHRKAGIAPPQPVVIVVEKLKSCGHTAYIAGGAVRDMIIDQRRP
                     VDWDIATSATPAEVSSCFEHVVPTGLAHGTVTVIEEGMSIEVTTYRHDGPYVDGRRPE
                     HVVFLDDIAADLARRDFTINAMAFDPTTGELVDPYGGVSDIEARLIRCVGDPAERFSE
                     DKLRMLRAVRFAATLGFSVDSSVVDAARALAPGIVQVSVERIMDELVKTVRAPKPGAA
                     LVLMEDMGLAPYILPELCCEVGEEERKALYAACDRLHPEPKERFAALLSPLGPNGARI
                     ALARMRVSNQFSITVSRIIEGAQNLSQNPSDYELRLTASRVGQDCLLPAVRLNRALCR
                     QGTGSLAVADLGSEGIEAVESLEERAGSVLAAKPALRIADLDISGKEVMEATGLSQGP
                     RVRIVLQRLLDQVLHDPSANKKECLLEMATGIAEEMERELA"
     gene            3618..4247
                     /locus_tag="PLM83_04725"
     CDS             3618..4247
                     /locus_tag="PLM83_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK70745.1"
                     /translation="MACEWVIQLSNEYAIARPAQPVSKATEFESLVRRHQNSVYNIAL
                     RMSGDPEEARDLTQEAFVRAYRSFDSYKRGTSFDKWLYRIVTNLYIDELRRRKRRPYI
                     ESLDQRITTSEGDELDKEIPDMSSNPETVYDRDHLDAFIQDALDSISPEYRMAVILCD
                     IEGFSYEEIAQIMGTSIGTVRSRIHRGRRALRERISPYLEERSREVSQK"
     gene            4244..4873
                     /locus_tag="PLM83_04730"
     CDS             4244..4873
                     /locus_tag="PLM83_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOK70746.1"
                     /translation="MTCNMAERLISAYIDHELSEDDRNRVRQHLAVCSSCMATYTATL
                     EVKRALSSMTPAQCPECVWEKVERQITLIPFDREMKRDDSTKPAWSFIKLVIPAAVAG
                     TLIVIPLVQALLGISLFGAPDKSQPPIASSKPNPQLELTSPSVRIHAAGTFSSSDALE
                     EVLGENIWLLEPTQASGKAANASQHGLDLRDIYDDYFMQVTSPERRYGR"
     gene            4870..6021
                     /locus_tag="PLM83_04735"
     CDS             4870..6021
                     /locus_tag="PLM83_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOK70747.1"
                     /translation="MKASFKKYLPLVVIAIISSVITSIVVVSYGFPERKGDTAAAVTP
                     IVASSYSGNLPSIADVAETVGPAVVRIDTERVVSYRSPFPDDGFFGPFFRDFFGGERI
                     QPGTGSGFIISPDGYIVTNYHVVEESRKVEVTLTDGRKFTARVVNGNKDADVAVIKIK
                     ANNLPVAQLGDSSKLRQGDWVVAIGNPYGFDHSVTAGVVSAVGRRIDDESGGTLATGD
                     LIQTDAAINSGNSGGPLINMEGKVIGINTAIIPYAQGLGFAISINSVKDTLKDIVNYD
                     EGPTPWIGIWYQQLDDDIAKQLGLSDANGILVTNVIQDSPAEKAGLKEGDVIKEIDGK
                     QITAKMSLAEEISKKKVGDKVVMYVLRGGQKHYLTVVLGEYPGDTLMLK"
BASE COUNT         1481 a         1822 c         1608 g         1383 t
ORIGIN      
        1 cgaacatcag cacattgcca cgcgctctag tcgccatgcg cgcagttatt tcgttttcct
       61 ctccgaatac ggcccattca tcctcagatt ggaaacggac gatattctcc ttcgcgaaga
      121 tgtagtcctc catgttcgga tggacgtcga ggtgatttgg ggttatgttg agcaccgcag
      181 ctatatgcgg gctgacggcc aagagcctca actgaaagct ggaaagctcc agaacaacga
      241 ggtcctcgct ttccactgat aatacgtgct ctattagtgg ggtgccaata ttgccgccta
      301 cgagcacgtc cctttcgggg tggtggaggc gtgcatcttc cgccaatatc cttcctgtca
      361 aggtagtagt cgtggtcttg cctgaactcc cggtaatgcc gatcaccggc gctgcagaaa
      421 gctccaatac gagctcgatc tcggagctga atgagactcc gacatcgcgc gcagccatga
      481 tctgcggttg ttccggattt actcccggag agaggaagac aatgtcgcaa tcggccagag
      541 aatcgagata tcccggtccg agcgagacat ctattcccag tcccgaaagg gacgccaact
      601 ccgaccccag ttgatctgct gtttttatat cgcaggcagt aaccttggca cctgcttggg
      661 caagaaagcg tatgagtggg acgtttgtaa tcccgatccc cactactgtg acccgctttc
      721 gggcaagcgt tcccataaca cgcttctgat tatctgtcaa catcatgaag gccccccaac
      781 atcaagtccc aaccccccgg catcttccta gactgtatct tgttgttctg cagtcgtgcc
      841 catgccgagg atcatttacg gaaataagtg cgctttggtc atcccctctg gcgcctccct
      901 atttcgataa gcctggccaa tgtttcagct gcatcagcca cgttggcata agcgcgcctg
      961 accgctgcgt ccaggtcgca cggccctttg accacggtaa gcagggcatc gattccgtga
     1021 tcatgtagtc ctctggcgtc gtcagccaca gacccggcca ataccgccac cggaacgccg
     1081 cactccgttg cgaccctggc aacccccata ggggtcttgc cataaagcgt ttggaaatcg
     1141 atacggccct ctcccgtaat gaccagatct gcacctgata ccttctcttt aagtcccgtg
     1201 gtctcaatta cgatttcgac cccgggctca agacgagcac cgaggaaggc cacaaggcct
     1261 ccgcccaatc ctcctgctgc ccccgcccca ggcatcgcct ctacgtcgat tctcaggtcg
     1321 cgccggatga cagaggcata gtttgcgaga gcagcgttga ggacttgaac catctccggg
     1381 gtggcgccct tttgcggacc gtataccgcc gacgctccat taggccctgt aagcgggttg
     1441 tccacgtcgc ttgccacaac gaattcggct ccaaccagcc gtcgatccat accggacgtg
     1501 tctatcctgg tgagcctcgc caactccgcg cctccaggtc ctatctcttt tccttcagcg
     1561 tccagcaatc gcgcccccaa cgcctgggcc atccctgccc cgccatcatt ggtggcgctg
     1621 cctcctatcc ctatgatgaa cttccggcag cccctgtcca gcgccgcggc gatgagttct
     1681 cctgttcccc gcgtcgtagc tgtcatagga ttccgcttgt ctaaaggcac aagcggtagg
     1741 cccgacgcgg ctgccatctc gatcacagct gtcgttccat cgcctaggat gccgaagaaa
     1801 gcctgcacct tgtcgcccaa aggtcctgtg gcttcaacat caataacttg accgccggta
     1861 gcatcaacta acgcctcaac ggtcccctct ccaccgtcgg ccatagggac acgcaccact
     1921 tcagcgtcgg gccacaccct gcgaagccct tcttctgcag ctcggcacac ttcggttgca
     1981 gcaagacttc ccttgaacga atccggagct atgactacct tcaacgatct ggccaaacac
     2041 aatcccccca aaaaacctta tcagcacaat ccctggcgag ctccatctta tgggcacaca
     2101 aataccatta gtcatcaacc cagcgcatct actgcgcatc tcaggtgtgc ctcctacgcc
     2161 aattcccgct ccatctcttc cgctatacct gttgccattt caagcaagca ttcctttttg
     2221 ttggccgacg gatcgtggag cacttggtca agcagccgct gcaaaacgat ccttactctg
     2281 ggtccttggc tgaggcctgt tgcctccata acctccttgc cactgatgtc gaggtcagca
     2341 attctcagcg caggctttgc ggccagcact gacccggctc gttcctccaa gctttcaact
     2401 gcctcgattc cctcactccc gagatccgca accgccaaag accctgtccc ctgcctgcac
     2461 aatgcgcggt tgagccgaac agccggaagc aggcaatcct gcccaactcg ggacgccgtc
     2521 agtctcagtt cgtagtctga tgggttttgg ctaaggtttt gagcgccttc aattattcga
     2581 gacaccgtaa tggaaaactg gttggagacc cgcattctcg ccaaggcaat gcgagcaccg
     2641 ttcggcccca atggcgaaag gagagccgcg aagcgctcct taggctctgg atgcaatcta
     2701 tcacaggctg cataaagggc cttcctttct tcttctccga cctcgcaaca caactcagga
     2761 aggatatagg gggcaagtcc catatcctcc atgagtacga gagctgcacc gggtttcgga
     2821 gcacgcacgg ttttcacaag ctcatccata atgcgttcca ccgatacctg tacgatccct
     2881 ggcgccaacg cccgcgcggc atccacaact gaagagtcta cactgaatcc gagcgttgcc
     2941 gcaaaacgta cagccctgag catcctaagc ttgtcttcgc tgaaccgttc ggcaggatcg
     3001 cccacacagc ggatgagcct ggcctcaatg tcggacacgc cgccataggg atcgactagc
     3061 tcgcctgtgg tcggatcgaa tgccattgca tttattgtaa aatcccgcct tgcaagatcg
     3121 gcagcgatgt catccaaaaa caccacatgc tccgggcgcc tgccatcaac atacgggccg
     3181 tcatgtctgt acgtggtcac ctctatgctc atgccttcct ctattactgt gacagttcca
     3241 tgagctaacc ccgtgggcac gacatgctca aaacacgaac ttacctcggc cggagtagca
     3301 gaagtagcga tgtcccaatc gacagggcgg cgctggtcga taatcatgtc ccgcacagct
     3361 ccccctgcaa tgtacgcggt atgtccacag ctttttagct tctccacaac aatcacaaca
     3421 ggctgtggcg gagcaattcc tgctttccga tgcatccaaa cacgcactcc ttccgcattc
     3481 tagtgtacgc acgcagacaa attccagcaa gtgcccattg aggatcctgt gtccctcaca
     3541 taccggcata gtttcaggta taattgacac agggaactcg cctcttgcgt cagtcgttat
     3601 tacgggtaga cgctcaaatg gcatgtgagt gggtgattca attgagcaac gaatatgcga
     3661 ttgcaagacc tgcacagccc gtgtcgaagg ccacggagtt cgaatctctc gttcggaggc
     3721 accaaaactc cgtttacaat attgcacttc gaatgagcgg cgaccctgag gaggcccgtg
     3781 atttgacaca ggaggctttc gtcagggctt acagatcgtt cgacagctat aagcggggaa
     3841 cctccttcga caagtggctg tatcgcatag tgactaatct atatatagac gaactgcggc
     3901 gtcgaaaacg gagaccgtat atcgagtctc ttgaccaacg aattactaca tccgagggag
     3961 atgaactcga caaggagatc ccggatatgt ccagcaatcc agagacagtc tatgacaggg
     4021 accatcttga tgcattcatt caagacgcgc tcgattccat ttccccagag tatcggatgg
     4081 ctgtcattct ctgcgacatt gaaggcttct cctatgaaga gatagcgcag ataatgggca
     4141 catccattgg gaccgtaaga tcacggatac accggggcag gcgagcgctc cgggagcgaa
     4201 tctctcctta cctcgaagag cggtcgcggg aggtgagcca aaagtgacgt gcaacatggc
     4261 tgagcgcctc atttctgcct atatcgacca tgaactctct gaagatgaca ggaacagggt
     4321 caggcagcat ctggccgtct gttcaagctg catggcaaca tacacagcaa ccctggaggt
     4381 gaagcgcgcg ctaagctcga tgacccctgc gcaatgtccg gaatgtgtat gggaaaaggt
     4441 ggaacgccag ataaccttga ttccctttga tagggagatg aagcgggacg attctacgaa
     4501 acctgcctgg tcattcatta agttggtgat tccggcggcg gtcgctggga cgctcattgt
     4561 tatcccattg gttcaggcgc tccttggaat cagcttgttc ggcgcaccag acaaatcgca
     4621 accgccgatt gcatccagca agcctaatcc ccagttggaa ctgacatctc ccagtgttcg
     4681 tatccatgca gccggaacat tcagttcaag cgatgctctg gaggaagtcc ttggggaaaa
     4741 catttggctg ctcgagccca cgcaggcttc aggaaaagcg gcaaacgcca gccaacatgg
     4801 tctggatctt cgtgatatct acgatgacta ttttatgcaa gtaacatctc cagaacggag
     4861 gtatggtaga tgaaagcatc ttttaagaag tatctgcccc tggtcgttat cgcgataata
     4921 agcagcgtga taacatcgat tgtagtggtg tcatacgggt tccctgaacg caaaggagac
     4981 actgccgcag cggtaacgcc gatcgttgcc tcaagctaca gcggaaacct gccatctata
     5041 gcagatgtag cagaaacggt agggccagct gtggtcagaa tcgacactga gcgagtggta
     5101 agctacaggt cccctttccc cgatgacggc ttcttcggtc cgtttttccg agatttcttc
     5161 ggcggcgaga gaatacagcc gggcacgggc tcagggttca tcatcagtcc agacgggtat
     5221 atcgttacca actatcacgt ggtagaagag tcccggaaag tggaggttac actgaccgat
     5281 ggacgaaagt tcactgcaag agtcgttaac ggaaacaaag atgccgatgt ggcggtcatc
     5341 aagataaagg ccaataacct ccccgtagcc cagctgggcg actcgagtaa gttgcgtcaa
     5401 ggggattggg tagtggctat cgggaatccc tacgggtttg atcactctgt tactgcaggg
     5461 gttgtgagcg cggtcggaag acgcatagac gacgagagtg gcggaactct ggccacgggg
     5521 gatctcatcc aaactgacgc ggccatcaat agcggaaaca gcggaggtcc gctcatcaac
     5581 atggaaggca aagtgatcgg cataaacacg gcgatcatcc cgtacgcaca gggacttggg
     5641 tttgccatat ccatcaattc cgtcaaagat acgctgaaag acatcgtcaa ttacgatgaa
     5701 gggcccaccc catggattgg aatctggtat cagcagctcg atgacgatat cgcaaagcag
     5761 ctgggcctgt cggatgccaa cggaatcctt gtgaccaatg tgatacaaga tagtccggca
     5821 gaaaaagccg gactcaaaga aggagatgtc atcaaggaga tcgacgggaa acagattacc
     5881 gcaaagatgt cgctcgccga ggagataagc aagaagaagg tgggcgacaa ggtagtgatg
     5941 tatgttctga ggggagggca gaagcactac ctcaccgtag tgcttggcga gtatcccgga
     6001 gacacgctca tgctaaagta atgctctgtc cagagtgttg aggcagccag acgttgtgcc
     6061 tcggggaatc gctaatggtt gtggcatatg gtcatatttc cccagaatct ttcgaagcca
     6121 aacttgaata agttccattt atgggagcag actatgagcg gcgggcagga taagacgtga
     6181 catatgggtt gtttctcacg gaaacgattt tctcacgcac ctgctcgctc cagattaagt
     6241 catcgccatg tccacccgtc gaaaagaagc cggccgggca gctgcagtct caac
//
LOCUS       DAOSAI010000101         6283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7924_length_6283_cov_3.931278, whole genome shotgun sequence.
ACCESSION   DAOSAI010000101 DAOSAI010000000
VERSION     DAOSAI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7924_length_6283_cov_3.931278"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1488
                     /gene="glyA"
                     /locus_tag="PLM83_04740"
     CDS             229..1488
                     /gene="glyA"
                     /locus_tag="PLM83_04740"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOK70748.1"
                     /translation="MYDNIRREDPEVADAISNEIRRQNEKIELIASENFVSEAVLEAL
                     GSPLTNKYAEGYPGRRYYGGCEHVDVVESLAIERAKRLFGAEHANVQPHSGAQANMAV
                     YFAALEPGDTVLGMNLTHGGHLTHGMHLNISGKYFHFIPYGVREDTQIIDYDAMENLA
                     IEHRPKMIVAGASAYPRIIDFERISEIAHSVDALLMVDMAHIAGLVAAGLHPNPVEYA
                     DFVTTTTHKTLRGPRGGMILCKSEWARKIDSAVFPGTQGGPLMHVIAAKAVAFAEALQ
                     PEFAQYQRGIIMNAQALADALMERGWRLVSGGTDNHLILMDLRGTGITGRVAEEALDE
                     VGITVNKNTVPFDPEKPMVTSGVRIGTPAVTTRGMKEPEMREIASLIDKTLRNLGSEG
                     VMDEVRQGVNDIISRFPLYSQIRAELS"
     gene            1645..2220
                     /locus_tag="PLM83_04745"
     CDS             1645..2220
                     /locus_tag="PLM83_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOK70749.1"
                     /translation="MAKLLEIRWHGRAGQGAKTAALVLAEAAISTGKYAQAFPEYGPE
                     RMGAPMKAYNRISDEPITVHHHVEEPDIVIVLDPTLLTVGNVKAGVPDDGIYLVNTPL
                     SPSEMRTRLGLEGSKAKVYTLDATQVSMDTIGRNIPNTPMIGALIAATGLLELDELLE
                     DTRRKLSKKLRPEVVDKNMEAIKRAYREVAS"
     gene            2220..2525
                     /locus_tag="PLM83_04750"
     CDS             2220..2525
                     /locus_tag="PLM83_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK70750.1"
                     /translation="MSVPKDAGWKDVPKAGRILEPGCADKYETGGWRTYRPVHIEENC
                     INCLFCWIYCPDQAILVEDGKFKGIDYAYCKGCGICAKECPGKRGNKALKMVLDEEA"
     gene            2552..3733
                     /gene="porA"
                     /locus_tag="PLM83_04755"
     CDS             2552..3733
                     /gene="porA"
                     /locus_tag="PLM83_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOK70751.1"
                     /translation="MSIRVGKTGNEAVAEAMRQINPDVVAAYPITPQTEIVQIFSSFV
                     ANGKVDTEFVTVESEHSAMSATVGASAAGARAMTATSANGLAFMWEVVYIAAAMRLPI
                     VMPVVNRALSGPLNIHCDHSDTMGARDSGWIQIFSENAQEAYDNTIQALRIAENSNVL
                     LPVMVTMDGFIISHAMEVMEILEDEKVREFIGEYVPENPLLDVDNPVTIGPIDLQDWN
                     FEHKRQQAEAMRNAKPVILEVAKDYAKLTGREYGLFEEYRMEDAEVAVVVLGSTAGTA
                     KAVVDELRADGVRAGLLKIRVFRPFPAEEIAEALKDVKAVAVMDRSDSFNAVGGPVFG
                     DVRSALYDVENGPRIVDYIYGIGGRDVTPVHIRTVYADLAKIADTGQVENLLTYLGVR
                     E"
     gene            3745..4671
                     /locus_tag="PLM83_04760"
     CDS             3745..4671
                     /locus_tag="PLM83_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK70752.1"
                     /translation="MATLKELSKKEVLISSGHRACAGCGFPVIIKQALLAAENPVVVS
                     CATGCMEVCTTIYPYTAWKVPFIHNAFENSASTLSGVEAAYKSLSRQGKIDKKIDFIA
                     FGGDGGTYDIGFQALSGAMERGHNMLYICYDNQAYMNTGIQRSSATPRGANTTTSPAG
                     TESYGKKQYRKDLSAIMVAHEIPYVAQASPSNWNDLVSKVKKALAVEGPAFMNIIQPC
                     VLGWKYPSDQSMELARLAVDTCFWPLYEVEDGEYRLTYKPKEKKPVEDWLKRQDRFRH
                     LFKPENAEIVKEIQEDVDERWAKLLAKCGEEA"
     misc_binding    5455..5771
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5842..>6283
                     /locus_tag="PLM83_04765"
     CDS             5842..>6283
                     /locus_tag="PLM83_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOK70753.1"
                     /translation="MLDLKFVRSNPELVRDSLRKRGESTEILDTLLACDERRRAILVE
                     AEELKRQRNEASAEVGRMKQTGEDASSIVSEMRMVSERIRGLDQELSEVEMRLAEMLL
                     GIPNIPHESVPVGRDESENVVVRKWGEPTAFDFEAKAHWDIGTDL"
BASE COUNT         1525 a         1458 c         1917 g         1383 t
ORIGIN      
        1 atcgttggcg cagttgcaaa ctatagggat gccaaagcgc ttgccgaggt ttcgaaaggc
       61 cttggagaag ccatgaaggg gatagatgtc tcgtccatgg ccgatgagga aaagctgcag
      121 ggcagaggct ggtagcgctg tctatagcat gtagattcgt ctggctttgg acctggcccg
      181 ggctttcgta agccgaaggc cagattttgc gaaggcgggg ataaatgcat gtacgataat
      241 ataaggcgcg aggatcccga agtagcagat gcgataagta acgaaattcg aaggcagaat
      301 gagaagatag agctgatcgc atccgagaac tttgtcagtg aagcggttct cgaggcgttg
      361 ggttcgccgc ttaccaacaa gtacgcagag ggttacccgg gtcgaagata ttacggcggg
      421 tgtgagcacg tagatgttgt cgagtctctt gctattgagc gcgcgaagcg gctctttgga
      481 gcggaacatg cgaacgttca gccacactcc ggtgcgcagg caaatatggc cgtttacttt
      541 gcggccctag aacccggcga tactgtcctt gggatgaact tgacccatgg cggtcattta
      601 acccatggaa tgcacctgaa tatttccgga aagtatttcc acttcattcc ttatggcgtc
      661 agggaagaca cgcaaattat cgactatgac gccatggaaa accttgccat agagcatcgc
      721 cccaagatga ttgtggcagg cgccagcgca tacccgagga tcatcgactt tgagcgcata
      781 agcgagattg cccacagtgt agatgcactt ctaatggttg atatggcaca tattgccgga
      841 ttggttgcgg ctggtcttca tcccaatccg gttgaatacg cggattttgt gaccaccacg
      901 acccacaaga ctcttcgtgg gcctcgcggg ggaatgatat tgtgcaagtc ggaatgggct
      961 aggaagatcg attctgcagt tttcccaggt acgcagggcg gtccgctcat gcacgtcata
     1021 gcggcgaaag ccgtggcttt cgcggaagct ttgcagcccg agtttgctca gtatcaacgt
     1081 ggtataatta tgaatgcgca ggctctcgcg gacgcgctga tggaacgtgg atggcgcctg
     1141 gtttcaggcg gaactgacaa ccatctaatc ctgatggacc tccgcgggac gggaattaca
     1201 ggaagagtcg ccgaggaagc cctcgatgag gtgggcatta cggttaacaa gaatacagtt
     1261 ccgttcgacc ctgaaaaacc catggtgacc agcggtgttc gaatcggaac ccctgctgtt
     1321 acgactcggg gcatgaagga gcctgagatg cgggagatcg cctcactgat cgataagacg
     1381 ctgagaaatc ttggctcaga gggagttatg gacgaagtca ggcaaggtgt gaatgacatc
     1441 atatctaggt tcccactgta ttcccaaatc agagccgagt tgagctgata tacaggcttt
     1501 ttgttgttga atttactcgc gaatcacttg ttgtgccgcg ggtacccgcg gcatgattca
     1561 tacgaactat acaagctgtg tacgagttac cccggccagc tatgtatgga tgaacgatga
     1621 acacaggata gggggaggaa ggttatggct aagctcctag agattcgctg gcatggacgt
     1681 gctggtcagg gggcgaagac ggctgcactt gtactggctg aggcagctat ctccacaggc
     1741 aaatacgccc aggcgttccc tgaatacggc ccagagagaa tgggcgcgcc catgaaagct
     1801 tataacagaa tcagcgatga accaatcaca gtccaccacc atgtggaaga gccagatatc
     1861 gtcatagttc tggatccgac tttgctcaca gttggcaatg tcaaggccgg agtccctgac
     1921 gatggaatct acctcgtcaa cacaccgctg tcgcccagcg agatgcgcac aagactgggg
     1981 cttgaaggca gcaaggcgaa ggtatacacc ctcgacgcga cccaggtgtc tatggacacc
     2041 attggccgca atatcccgaa cactccaatg atcggtgccc ttattgcggc aacggggctt
     2101 ttggagctcg atgagctgct tgaggatact cggagaaaac tctccaagaa gttgcgcccc
     2161 gaggtggttg acaagaatat ggaggctatt aagcgggcct atcgggaggt ggcttcttaa
     2221 tgtcagttcc taaggatgcc ggctggaagg acgtgccgaa ggccggaagg attctagaac
     2281 cgggatgtgc cgataagtat gaaacaggcg gatggagaac ttaccgccct gttcatatag
     2341 aagaaaactg catcaattgc ttgttctgtt ggatttactg ccctgatcag gccatccttg
     2401 tagaggacgg gaagttcaag ggcatagact acgcttactg caaaggctgc ggcatatgcg
     2461 caaaggaatg cccaggtaag cgtggcaaca aggcactcaa gatggtcttg gatgaagagg
     2521 cctagacgaa gtaaggaagg aggagcaatt catgagcata agggttggaa aaaccggaaa
     2581 tgaggcagtc gcagaggcga tgcgccagat caacccggac gtagtcgcag cgtacccgat
     2641 tacgccgcag accgagatag tacagatctt ctccagcttc gtcgctaatg gcaaggtgga
     2701 taccgagttc gtgacggtag agagtgagca ctccgcgatg agcgctactg ttggagcctc
     2761 tgcagccggc gcgcgagcaa tgactgctac atcggcaaat ggtcttgcgt tcatgtggga
     2821 ggttgtatat atcgcggccg ctatgaggct gccgatagta atgccggtcg tgaatagggc
     2881 gctttcgggg ccgctgaata tccactgcga ccatagcgac acgatggggg caagggattc
     2941 tggatggatc cagattttct ccgaaaatgc gcaggaagcg tatgataaca ccattcaggc
     3001 gcttaggatc gcggagaatt ccaatgtgct gctgccggtc atggtgacga tggatggttt
     3061 cattatcagc catgccatgg aagtaatgga gatcctcgaa gatgagaagg ttcgggagtt
     3121 cattggagaa tacgttcccg agaaccctct cctcgacgtg gacaacccag tgactattgg
     3181 acccatagat ctgcaggatt ggaatttcga acataagaga cagcaggctg aggctatgcg
     3241 caacgcgaaa cccgtgattt tagaggttgc taaggattac gcgaagctga ctggtcgcga
     3301 gtatggcctg tttgaagaat acaggatgga agatgccgag gtagctgtgg tggttcttgg
     3361 atctactgcg ggtactgcca aggctgtcgt ggatgagctg agggccgacg gggttcgtgc
     3421 cggcttgctc aagataaggg tattccggcc attcccggca gaagagatag ctgaagcgtt
     3481 gaaagacgtg aaggccgtgg ctgtgatgga cagatcggat tcgttcaatg ccgtgggcgg
     3541 cccggtcttt ggcgatgtgc gctctgctct gtatgacgtc gagaacggtc cgaggatcgt
     3601 tgactatatc tacgggatcg gcggccgcga tgtgactcct gttcacatcc gaacggtcta
     3661 tgccgaccta gcaaagatcg ccgacaccgg gcaggttgag aatctcctca cctaccttgg
     3721 ggtcagagag tagggggtgc gcacatggcg actttgaagg aactgtctaa gaaggaggtc
     3781 ttgatctcat caggccaccg tgcctgtgcg ggttgcggat ttccggtcat tataaaacag
     3841 gcgctcctgg cggcggagaa tccggtggtt gtgtcgtgcg caacaggatg catggaagta
     3901 tgcaccacta tctacccgta tacggcgtgg aaggttccat tcatccacaa cgcgtttgag
     3961 aattctgctt ccacccttag cggcgtagag gcagcataca agtccctctc gcgtcagggc
     4021 aagatcgaca agaagatcga cttcatagcc tttggcggcg acggaggaac gtacgacatc
     4081 ggtttccagg cgctttccgg agctatggaa cgtgggcaca acatgctata catctgctac
     4141 gacaatcagg catacatgaa tacagggatt cagcggtcga gcgcgactcc gaggggtgct
     4201 aacaccacta ctagccctgc gggaaccgag tcgtacggga agaagcagta tcgcaaggat
     4261 ctcagtgcaa tcatggttgc tcatgagatt ccctacgtgg cgcaggcttc cccaagcaat
     4321 tggaatgact tggtcagcaa ggtaaagaag gccctggccg tcgagggccc tgccttcatg
     4381 aacatcatcc agccatgtgt tctcggatgg aagtatcctt ccgatcagag catggaactg
     4441 gccaggttgg ctgtggatac ctgtttctgg ccgttgtatg aggtggaaga cggagaatac
     4501 aggctcacct acaagccaaa agaaaagaag cctgtggaag attggcttaa gaggcaggat
     4561 agattcagac acctcttcaa gccggaaaac gccgagatcg tcaaggagat ccaggaggat
     4621 gtggacgaac gttgggccaa gctcctggcc aagtgcggcg aggaggcgta acagcctgac
     4681 agtccataca gcttaatagg ctgttgtttt cactaacgag cgtgaagcca gctggtcgtc
     4741 tcaatgtcgg ggcgcctggc tggctttatg aatgtgctga gagtctgcgc cgtgccgcag
     4801 gcattggcgc agttaagggg agcaccgcct tagacgtgca aatatcgttg caatgcagcg
     4861 tttcgtaggc tcgcgattga ggcatgccct gggcgaacct tgtgatagcg agtttacatg
     4921 ttaagcaaca gctcaaagcg gctatcttgt tctcagccca aaccccttgt ttcgtcagga
     4981 attgagcaca cctcatttta cgaagcatgc tggagtattg cgtcatggtg ggtgggaatg
     5041 attgccagta cactgcgatt gcgacttatc aggagagcag tatgaggggg caattctctg
     5101 tttaggtgcg gaaaggatct ccgcacccga ggttgaaggt attcgaattt gatacgctaa
     5161 gtgaaggcga aggtgcggca tgtaatcagg gtatgtgagc ttttcgatat ggtcaacgga
     5221 gacaagatcc taatcggcat gcttgggctt gcgtccggca ttgttatgcc ctagggcctg
     5281 gattccgtac gaagaccctc ttccgtaatt tttccgtcaa gatgatgtcc cccttgttca
     5341 tgggtgacat tatctcagac gcgtaacaca ttttcgttgg ccccccttga ctttgcagga
     5401 ttgtggatat atcatataag gaagttgcag gataccgttt ctcctgcata tacagcaaac
     5461 gcgatgagag ggaatagtat cccgcagtct tctttgcaga gagccggggg cgccttcaga
     5521 ccatttgagc ggaagctacg ctcagatgtt tccgagtctg tgggggctgg tgggaaccgg
     5581 taagaggcag tgggggaatc caccctggag cggccggccg aacaagagta agccgtgacg
     5641 ggatcgcccg ttatagcgga cacgagaggg tttgcctgag atcaagcgcc cagcgcctaa
     5701 gggcgtgttt gccgtgcttg tccaggcgac caatcggggt ggcaacgcgg gaagtttgtc
     5761 ccgcccccgg cggcgaaagc tgcgaggggg cgggattttt ttgtaggcgg gatgggcaat
     5821 agatgaaggg ggagtcttag tatgctggac ctgaaattcg taaggtcaaa cccggagtta
     5881 gtaagggatt cgctgcggaa gcggggagag agcactgaaa tactcgatac tctcttggct
     5941 tgcgatgagc ggcgtcgggc tatactggtt gaagctgagg aactaaagcg ccaaagaaac
     6001 gaagcatctg ctgaggtcgg gcgcatgaag cagacgggtg aggatgcatc cagcatagta
     6061 agtgagatga gaatggtctc tgagcgcatc cgaggtttgg atcaggaatt atctgaagtg
     6121 gagatgcgcc tagccgagat gctgcttgga atcccgaaca ttccccatga atctgttccg
     6181 gtaggccgtg atgagagcga aaacgtggtc gtgcggaaat ggggagagcc gacggccttc
     6241 gatttcgagg ccaaagccca ttgggatatc ggtactgacc tgg
//
LOCUS       DAOSAI010000102         6283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7925_length_6283_cov_3.833976, whole genome shotgun sequence.
ACCESSION   DAOSAI010000102 DAOSAI010000000
VERSION     DAOSAI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7925_length_6283_cov_3.833976"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="PLM83_04770"
     CDS             complement(<1..1336)
                     /locus_tag="PLM83_04770"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOK70754.1"
                     /translation="MGDKEVDPILSMEGICKSFPGVKALDGVDFSLWPKEVHALVGEN
                     GAGKSTLIKILAGVYKMDSGKILVEGRPVDISSPRQAQEHGISVVHQEFTLVPQMTVA
                     ENIMLSNMPSIPGLRSLVDKKTMVYESEKILNGLGVDIDVFAPVRLLTMAEQQIVEIA
                     KALSGKTRILVMDEPTAALSNVEVERLFDVIRTLVSKGIAVIYISHKLEEVFRISHRI
                     TVLRDGRVVSSSPISEVTRNEVVKQMVGRELTTMYDRCKADIGGPAMEVKGLLLDPAG
                     EPISFTVHEGEILGIAGLMGAGQIQLARALFGIGRTYGGEVLLRGRPASLSSPSKAVR
                     SGVGLVTENRNEEGLVQLLSVSKNITITVLDAISRRGVLSSWMEDRIVTEKIEELDIR
                     TPSPKQPVMNLSGGNQQKVVLAKWLAVDPDVMIMCEPTRGIDAAENEKSVLGP"
     gene            complement(1317..>1511)
                     /locus_tag="PLM83_04775"
     CDS             complement(1317..>1511)
                     /locus_tag="PLM83_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70755.1"
                     /translation="ALACVLDESLGGSIDIATLIAHAQAAAAISTTSLGAQTSMPIRT
                     EVEEFLAERGGGSGHGRQRG"
     assembly_gap    1512..1521
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1522..2329)
                     /locus_tag="PLM83_04780"
     CDS             complement(<1522..2329)
                     /locus_tag="PLM83_04780"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOK70756.1"
                     /translation="MSIVVVGSCNMDYMMQVTSLPKRGETIFAVSHSTAAGGKGANQA
                     VAAARLGSRVYMVGRVGSDEAGRVLVESLEMAGVDASKVGKDVMESTGSAYITVDRDG
                     ANTIVVNRGANFAIKPSHVTAAESLIQEADVVVVQLETPIEVVFQALRLARKHGAVTV
                     LNPAPMAPLKGEMLALADWLIPNETEAFSLLRSLGLSREPNGKIVKGRDPAIDVARRL
                     ANVTGSNVVVTFGDKGCAYAGPLDREGIAFPAYEVDAIDTTGAGDTFVGAL"
     gene            complement(2326..3357)
                     /locus_tag="PLM83_04785"
     CDS             complement(2326..3357)
                     /locus_tag="PLM83_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK70757.1"
                     /translation="MRKWWVAISTIYEVAQKAGVSVATASRALNDSGYVSRESRDRVL
                     AAAKALDYHINVNARSLTSGKTYTIALVLPDVTNPFFPAVARGVEDYASDNGYCVILC
                     NTDGNARKESDYVRMLRSRKVDGVIFAVSVASKSRVSALTDDGIAVVLVDRDLGDSCD
                     IVKTDNIAGALLATNHLIGLGHTSIAFISGPMSLATSQERFRGYHDALVSGGVSFDER
                     LVVEGDFRMKSGYEAMRDLLRKPCQFTAVFAANDLMAVGAMTALEESGFHVPTDVAVM
                     GYDDIDIASVTRPRLSTVAQPKYEMGWIAAETVIRRMCNPKASRMEELLQPMLVVRDS
                     TVERRGASV"
     gene            3899..4927
                     /locus_tag="PLM83_04790"
     CDS             3899..4927
                     /locus_tag="PLM83_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOK70758.1"
                     /translation="MSTKDSPYVIGVDGGGTKTLAVAATLAGEIIAAARSGPANYQSA
                     GKDVAASNIRQVISEIAEITSAPRGCLAVYYALSGADRPLDFEIIEDMIGGITPSPPW
                     PHWEVDNDALAPLALGSEDGTGVVVICGTGTNCIGINATGQRKQVGGLSPAFGDAAGG
                     SVIGIRAYSAAIRGEDGRGRPTLLSQLIRSHIGSSDLLSVVHHTYADGEGFAFSSIAP
                     LVFEAAERGDSVAVEILIDNGRELGISALAALRELFSPEDDPTVVLAGSIAQNPNPIT
                     LTAVREAVLPEYPNARIMVPTAEPVLGAVLLATRLAGKGLAVESSREFMKRLEHSYST
                     FDRSESLQ"
     gene            4924..6186
                     /locus_tag="PLM83_04795"
     CDS             4924..6186
                     /locus_tag="PLM83_04795"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOK70759.1"
                     /translation="MKLTLVGAGSTYTPELVDGLIRRRQDLPVETLSLMDIDKERLTT
                     VGSFVKRMVDASGESLNIEMHTQSCDAIVGADFVVTQIRVGGQLWRDLDCKLAMEHGL
                     IGQETTGFAGFAKAIRTIPPILEICKTMREAAPDAWLINFTNPSGIITEAIARHGRVR
                     AIGLCNVPINMKLEAARALGVPANEISLDYIGLNHLSWIRKVHWLGRDVTDQALAIAA
                     SSMQKNISGTPATPKLAEALSMVPSPYLKYFYRPDASIAEQKSSPKTRAEEVMEIEAE
                     LMRIYKDPAVVSKPPALAKRGGAHYSTVSIDIMSSVYNDSGKVEIVDIPHAGAVDGFP
                     ASSVMEIPAVIDATGAHPIHIGSIEPEIRGLMHQVKAYEELTIEAAVTHSYNRALLAL
                     INNPLVQDAGKAERLLDALVESKRASLR"
BASE COUNT         1488 a         1896 c         1515 g         1374 t
ORIGIN      
        1 gcgggcctaa taccgacttt tcgttttcgg ctgcatctat gccgcgcgtg ggctcacaca
       61 tgatcatcac atcagggtca acagcaagcc actttgcaag tactaccttc tgctggtttc
      121 cgccggacaa gttcatgaca ggctgctttg gagacggcgt tcttatgtcc aactcctcga
      181 ttttctcggt aacaatgcgg tcctccatcc aagaagagag cacacccctg cgggaaatgg
      241 catccagaac cgtaatggtg atgttcttcg aaactgaaag cagctgaaca aggccttctt
      301 catttctgtt ttcagtcacc aagcccacac cggatctcac tgccttgctc ggagatgaga
      361 gactagccgg tcttccccgg agaagaacct ctccgccgta cgtccgccct atcccgaaaa
      421 gagctctcgc caactgaatc tgaccggcgc ccatcaagcc tgcgattccc aggatctcgc
      481 cttcgtgaac tgtgaaggat ataggctctc ctgccggatc caaaagcaag cccttaactt
      541 ccattgcagg acctccaatg tccgccttgc atctatcgta catcgttgtc agctcgcggc
      601 ctaccatctg tttgacgact tcgtttcgcg taacttccga aatcggcgaa cttgaaacga
      661 ctcgcccgtc tctaaggaca gtgatcctgt gagatattcg aaagacttct tcgagtttgt
      721 gggagatata aataacagct atgcccttcg aaaccagggt tcggatgaca tcgaacagcc
      781 gctccacctc gacgttagac aaggctgctg taggttcatc catcacgagg atgcgggtct
      841 ttccagacaa cgcttttgca atctccacta tctgctgctc ggccatggtc agtaaccgga
      901 caggcgcgaa gacgtcaatg tccacaccga ggccgttgag gatcttctca gactcataga
      961 ccatggtttt cttgtcaacc aagctcctga gcccaggaat agatggcatg ttcgatagca
     1021 tgatgttctc agcaacagtc atctgcggca cgagcgtgaa ctcttggtga accacgctaa
     1081 tgccgtgttc ttgagcctgg cgcggagaac tgatgtcaac tggccggcct tcgaccagga
     1141 tcttcccgga atccatttta tatacgccag ctaagatctt gatgagagtg gactttccgg
     1201 ccccgttctc gcctaccagc gcatgcacct ctttgggcca taggctgaaa tcgacgccgt
     1261 ccaaggcttt tactccggga aagctcttgc atatgccttc catgctgagg ataggatcaa
     1321 cctctttgtc gcccatgtcc actacctcct cccctttccg ccaagaattc ttccacctcg
     1381 gtgcggatag gcattgaggt ctgcgctcca agactagtag tggagatggc tgccgctgcc
     1441 tgcgcatggg caataagcgt ggcaatatct atcgaacccc ctaagctctc gtccaacacg
     1501 cacgcgagcg cnnnnnnnnn ncgagcgctc caacaaatgt atcgcccgcc ccggtcgtgt
     1561 ctatcgcgtc tacctcgtaa gctggaaagg cgatgccttc gcgatcgagc gggcctgcat
     1621 atgcacatcc tttgtcccca aaagtcacaa ccacattaga gcccgtcaca ttcgccagcc
     1681 ttctggcaac atctatagca ggatccctgc ctttcactat cttgccgttt ggctcccgcg
     1741 atagacccaa cgaacgcagc aaggagaacg cctccgtctc atttggaatc agccagtcgg
     1801 caagcgccag catctcccct ttcaacggcg ccattggagc aggattgagc accgtcacag
     1861 caccatgctt tcgtgctaac ctcaaggcct ggaagactac ctcaatcggc gtctccagct
     1921 gcacaacaac aacatctgcc tcctgaatca ggctttcagc ggccgtcaca tgtgaaggtt
     1981 ttatggcgaa attggcgccc cggttcacga cgatggtatt agctccatct cgatccacgg
     2041 ttatataggc agaaccggtc gactccatca cgtctttgcc taccttcgaa gcgtctacac
     2101 cggccatttc caggctctcc acaaggaccc gccccgcttc gtcagatccc acacgtccaa
     2161 ccatgtatac ccgagatccc agcctcgcag cggcaaccgc ctggtttgct ccctttcccc
     2221 ctgctgcagt agaatggctg acagcaaaga tcgtctcgcc acgtttcgga agactggtga
     2281 cctgcatcat gtagtccata ttgcaacttc ccacaacgac tatgctcaca ccgaagcacc
     2341 tctcctttcc acagtggaat ccctgacgac caacataggc tgcaaaagct cctccatgcg
     2401 agacgccttg ggattgcaca tccgcctgat gaccgtctcc gccgcaatcc aacccatttc
     2461 atacttgggc tgcgcaacag tcgacagacg cggacgcgtc accgacgcaa tatcaatatc
     2521 gtcgtatccc ataacagcca catcggtcgg cacatggaat ccgctctcct caagcgctgt
     2581 catcgcgccg accgccatca ggtcattggc agcaaagacg gcagtgaact ggcacggctt
     2641 tcttagaagg tcgcgcatcg cctcatatcc gctcttcatg cggaaatcgc cctcgaccac
     2701 caatctttca tcgaaggaaa ctcctcctga aacaagcgca tcgtggtatc cccgaaaacg
     2761 ttcctgagat gtcgccagcg acatggggcc ggagataaag gcgatactgg tgtggccgag
     2821 gccaataagg tgatttgtgg ccagtaatgc tcctgcgata ttgtcggtct tcacaatgtc
     2881 gcatgaatcg cccaaatctc tgtcgactaa gactaccgca attccatcgt ccgtcaaagc
     2941 gcttaccctc gacttcgacg ccacgctgac tgcgaatatc acgccgtcca cttttctgga
     3001 ccgcaacatc cttacatagt cagactcttt gcgcgcgtta ccgtctgtat tacacagtat
     3061 tacgcaatat ccattgtcag aagcgtagtc ttcaacaccc cttgcaaccg caggaaagaa
     3121 tgggttggtg acatcgggca gaaccagagc gatggtgtac gttttcccgc tggtaaggct
     3181 gcgcgcgttg acgtttatgt gatagtccag tgcttttgca gcggccaaga cacggtccct
     3241 cgactccctg ctcacgtaac cgctgtcatt gagcgcccga gaggcagtgg cgaccgatac
     3301 tcccgctttc tgcgccacct catatatggt gcttattgca acccaccact ttctcataac
     3361 tggttgtctg gcaaccgctt ctctcgcctt atgatgagaa atctatattg cagattctag
     3421 gcatcaacac agacaccgtg taacaggttt cacactacta accgcccttg cctcgacgtt
     3481 tttctgtagc gtcatggcaa tgtgtatgcg gttccacatt tatattccat gtacagtatg
     3541 caattccttc cttgtgtcaa taattatttg gaatggccga caagttatgc cgagggcgcg
     3601 ttcacgaata cgcggttggc gggttctgcc tgtgctttag ccacagtgga tacaaaacgg
     3661 tgggacaatt cgattatcac tctttctctc ggccagcgtc tcttgtagat ctcgaatgcg
     3721 gcggacataa caaagaggct acccgctccc tgatatctcc acagccatgg ccaccaagta
     3781 aacttgggca gacaaagacg gacaggagga cgcccacttg tggagaatcc tctcgtggac
     3841 atcatcgtga gactatcttc gcaatctacc gactaagaac aaggcaggta tcaatggcat
     3901 gtctaccaag gactcaccat atgtaatagg tgttgacggc ggaggcacca agaccctcgc
     3961 agtcgccgcc accctcgcag gagagattat cgctgcggca agatccggtc ccgccaacta
     4021 ccaaagcgca ggcaaggatg tagccgcatc caacatcaga caggtcatct ccgaaatcgc
     4081 cgagataaca tccgctcctc gaggatgcct cgctgtctat tacgcgcttt ccggagcaga
     4141 tcgtcccctc gacttcgaga tcatagagga catgataggc gggataacgc ccagtcctcc
     4201 gtggccgcac tgggaggtgg acaatgatgc ccttgcaccc ctcgcactag gctcagagga
     4261 tggcactgga gttgtcgtga tctgcggcac aggaaccaat tgcatcggga taaatgctac
     4321 cggccaaaga aaacaggttg gcggactgag tcctgctttt ggcgatgccg caggcgggtc
     4381 tgtcatcgga attcgagctt actccgccgc catcagaggc gaagacggac ggggacgccc
     4441 cacgcttctg tctcaattga tccgtagcca tattggctca tccgatttgc tatcagtagt
     4501 ccaccatacc tacgccgatg gcgaaggctt tgccttctca tccatcgccc cgctggtgtt
     4561 cgaagcagca gaacggggag acagcgtagc cgtggagata cttatcgaca acgggcgcga
     4621 gctaggaata tcagcccttg cagccctgag agagcttttt tcgccagaag atgatccaac
     4681 agtcgttctg gcaggcagca tagcccagaa cccaaacccc atcaccctca ccgccgtacg
     4741 cgaggcagta cttcccgaat accccaacgc acgcattatg gtccctaccg ccgagccagt
     4801 cctcggcgcg gtcttactcg caacacgcct ggcaggcaag ggtttggctg tagaatccag
     4861 cagggaattc atgaagaggc tcgagcactc atattcaacc ttcgacagga gtgagagctt
     4921 acaatgaaac tcacattagt aggcgctggc agcacttata caccggagct cgtggacggc
     4981 ctcatccgtc gccgccagga cctccccgtg gaaaccctca gtctcatgga tatagacaaa
     5041 gaacgtctca ccaccgttgg ctctttcgtc aagcggatgg tagacgcatc cggggaaagc
     5101 ttaaacatcg agatgcatac acagtcctgc gatgcaatag tcggcgctga ctttgtagtt
     5161 acccaaatac gcgtcggagg ccaactctgg cgagatctcg actgcaaact cgcgatggaa
     5221 cacgggctca tcggccaaga gaccaccggg ttcgcgggat ttgccaaggc tataaggacc
     5281 attcctccca tcctcgagat ctgcaaaacc atgcgcgaag cggcccctga cgcttggctg
     5341 atcaacttca ccaacccctc cggaatcatt acagaagcca ttgcgcgcca cgggagagta
     5401 cgcgcgatcg gcctctgcaa tgttcccatc aacatgaagc tcgaggcagc tcgagctctg
     5461 ggagtcccag cgaacgagat ctccctcgat tacattgggc tgaaccactt gagctggata
     5521 cgaaaggtgc attggctggg cagagacgta accgatcagg cgctggccat tgcagcctct
     5581 tccatgcaga aaaacatatc cggcaccccg gcgactccaa agctggcaga ggcgctcagt
     5641 atggtgccat ccccatacct gaaatacttc taccgcccgg atgcctcaat agcagagcaa
     5701 aaatcttctc caaaaacccg ggcagaagag gtaatggaaa tagaggcaga gctgatgaga
     5761 atctacaaag atcccgctgt agtctcaaaa cctcctgccc ttgccaagcg aggcggcgcc
     5821 cactattcca ccgtctccat agacataatg tcgtctgtat acaacgactc ggggaaggtc
     5881 gagatagtcg acatccctca cgcaggcgcg gtcgacggct ttccggcgtc atcggtcatg
     5941 gaaattccag ccgtaattga cgccacaggc gcccacccca ttcacatcgg cagtattgag
     6001 cccgagatac gcggactcat gcatcaggtc aaggcttatg aggagctcac catagaagcg
     6061 gctgtcaccc attcatacaa ccgagctctc ctagccctga tcaataatcc attggttcaa
     6121 gatgcaggca aagctgaaag actcctggat gcgctggttg aatcgaaaag ggcgtcactg
     6181 cggtaagcag cctcccagtc ttgatgagga gccaaagcct attccgcaga gctacaagct
     6241 tcaacatcgc gcagacacgt gtcgagactt gcttaaaaca cag
//
LOCUS       DAOSAI010000103         6243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_7986_length_6243_cov_3.824014, whole genome shotgun sequence.
ACCESSION   DAOSAI010000103 DAOSAI010000000
VERSION     DAOSAI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7986_length_6243_cov_3.824014"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLM83_04800"
     CDS             <1..340
                     /locus_tag="PLM83_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOK70760.1"
                     /translation="KWVIHTVGPVWHGGGLGEAELLTNCYRNALRLAVENGARTVAFP
                     SISTGAYRYPVDEASRIAIRTVSEFLSVDDTIEDVRFVCFDTHTYSAYLRALAEHLGT
                     QGLPRNSASP"
     gene            complement(824..1099)
                     /locus_tag="PLM83_04805"
     CDS             complement(824..1099)
                     /locus_tag="PLM83_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70761.1"
                     /translation="MRVTGLILGLIGSIWGMFVPVDDLRWLVVLISIAGLIAACCAMA
                     YPKWAGIIMLIAAVLGWIFGKGDFGWPGAILLIGGIFALSGQGELES"
     gene            1711..2754
                     /locus_tag="PLM83_04810"
     CDS             1711..2754
                     /locus_tag="PLM83_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK70762.1"
                     /translation="MRPTLQDMADILNLSKSTVSRALSGDPRVAQATRARVESLAERI
                     GYTPNPIARGLATRRTNTIGLAVPCAPRSLSDPFYLEFLGHAGNAAMKCGYSLLLSAA
                     EEDGADTHNAHVRLASSARIDGMILTEPKIKDERIAALKKAGIPFVFLGMTSDPDVSW
                     VSCDNKLGAAEAVAWLTGLGHTRIACLTGPADQTASDARYRGYCEALDEAGIPLDLNI
                     VASGDFTQAGGHAAMREILERRKTVSAVFASNDVMALGAMCALRESGLSVPEDVSLVG
                     FDGIAFAQYSTPPLATVQQPIDELGNASVEVLIKLMCGDNGGPIHRVMPARFRPAGSV
                     AAPGRAFVLESPD"
     gene            2902..4083
                     /locus_tag="PLM83_04815"
     CDS             2902..4083
                     /locus_tag="PLM83_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK70763.1"
                     /translation="MKRKMIVLLILALAICSLPGATAEKKLTVISGWSGPEMDAFMPV
                     LKAFEKETGIRVDYQIYRAEDLAVLLPAQFNAKTTPGDVIYMWSWFITKHAKDGHVLE
                     MTGSLKDADFIPGALDAVKVDNKLWGGVYTGKVKPGFWYRKSFFKKHGLSEPKTWDEF
                     VALCAKLQKISGVKAPIASGNGVGWPLSDITEHFIATYAGPQVTYGLIDKKVKWTDPV
                     VKSVFTDRIVPLLKAGYFSEPIEWTMALDLWWRGDYGLYFMGSWITGMVDNPDDLGVF
                     SLPGCEGIVFAPDYAFVPAYTKYPAEARKLLAFLSTKGQEIQVSTGGHIATYKVPLNA
                     YPPVDRGVASLLKGVVALPDLDDTIGGEWQAAFWDQLKLLWVSPGRVNEVLENLQRKA
                     N"
     gene            4180..5052
                     /locus_tag="PLM83_04820"
     CDS             4180..5052
                     /locus_tag="PLM83_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK70764.1"
                     /translation="MGQTRGRTLFFIPALALLALFVVYPVANTIWLSFFTPEGEFAFF
                     KNYADVLSQPEIFDPRGFERGFPYGALIHNVIWIVLHLPLTVILGLVFAVILRDIKGG
                     AIVKSIVFLGMVTPMVVGGIILRFLFDGNVGIIPSVLALFGFAPRTLTAYPDTALFSL
                     IAGSLWLWTPFSMVLYSAGLETIPDFVYEAAVIDGASPWRIFFNITVPLLKPVTGVVV
                     TMTFLWVLKIFDIVFVATMGGPGGASNVLALQMYMYAFREFNPNAAAVVATFLMIVAL
                     VVSIPTFKAAGGEE"
     gene            5049..5894
                     /locus_tag="PLM83_04825"
     CDS             5049..5894
                     /locus_tag="PLM83_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK70765.1"
                     /translation="MMRTVGCSSGHKVITYVAAGLIALIWLIPFLGVFMASIRPLSEI
                     NRGWWRFDSFTPTFKNYVGAWNHPAAPISEGMLNSLKVAIPATIIPVFVASIAAYGFA
                     RFKFRGRDQLFILVVALMTLPQQMIAVPMFQMMNKLGAVDSYSGLILVHSAWGIPWAL
                     YFMRNYFTTLPIEVEEAAKVDGASDWRIFFQIIMPMALPAIASAAVLQFMWVWSDFFL
                     ALILIYSPAKLLATQRVPLLRGIYHVDWGILAAGSIIVMAVPIIVFAVMQRYYIRGMV
                     GWVSK"
     gene            5930..>6243
                     /locus_tag="PLM83_04830"
     CDS             5930..>6243
                     /locus_tag="PLM83_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HOK70766.1"
                     /translation="MFRKVDFLEKSLDDHAPYAGEDRIAEIRRLAEDLGSLRVLHVNS
                     TSYGGGVAELLYTIVPLMRDAGLDAEWHVIDGAPDQFFDITKKIHNSLQGANTPLSDG
                     EWR"
BASE COUNT         1437 a         1450 c         1745 g         1611 t
ORIGIN      
        1 caaatgggtg atacacacgg tcggtcccgt atggcatggc ggtggactcg gcgaagcaga
       61 attgctgacg aattgttatc ggaatgctct gcgtcttgct gtagaaaatg gtgcgcgcac
      121 tgtggctttt ccgtcgatca gcactggcgc ctatcgttat ccggtagacg aggcatcgcg
      181 aattgcgatt cgtacagtat cggagttcct gtctgtagat gacaccatag aggatgtcag
      241 attcgtctgt tttgacacac atacgtattc cgcgtacctg agggctctcg cggagcacct
      301 gggcactcag ggcctgccgc ggaactcagc gtcgccatga cggagtctgc tcgttagcag
      361 attgtggtcg gcatgttcgg cgggttaatg gtatactctt gcgttggcag tggatttgtg
      421 caagggagat atgtggagtt tgccggtgat gtttggcatc acgattaaca cttcactagc
      481 ttagccgtat ccggctttgc tcagctcagt ctgtagcgac tcttctgtca aatggagagc
      541 attgaggttt tcgtagtgca cttttctctc caactctacc gtctgagcga ggccgaagaa
      601 ccttataatg cagctctcat gacagaagat gccatgatgt tcggaatgtc aacgcaaaac
      661 acataatact atgaataaaa agttcaattg ccgtaacaag tgtctgcgac ttcagtggtg
      721 gagatcgagt aaaaaagagg ggaatttcga cgcaatagcg aggccgacgt cgtttcccct
      781 ctacttgtaa ttaagttatg agtccgttaa ttgctgattt tggtcagctt tctaattccc
      841 cttggccaga taaagcgaag attcctccga taagaaggat tgcgccgggc caaccgaagt
      901 cgcctttacc aaagatccag ccgagaacag cggcaataag catgatgatg ccagcccatt
      961 tcgggtacgc cattgcacag caagctgcaa tgagtcccgc tatggagata agaacaacaa
     1021 gccatctcag atcatcaact ggaacgaaca taccccagat tgaaccaatc agcccgagta
     1081 tgagtccggt aactcgcatt ctagtccctc ctcacattta atcactcttg tgcctggttc
     1141 agtacaaacg cgtcggcctc gccacgtccg acactgacct tggacacaac ttggaaacca
     1201 aatcatacgg cttccaacta ttagcttaat cgttctctcc aatactgtca accgaagaag
     1261 atgaattctt acgcaatatt gcgcaaacac gctaaaaacc acgagataaa tgcagaatcc
     1321 acgtcggttg tacccccgaa aaggcggtgg gcaagggaag atctcgtgac attttctgtt
     1381 agcggggcag cttggcgtgt atgcccttgg ggccctggag gcggcaagag taatcttggt
     1441 gattgagaag gcaagataac accgggaatg agggcattgc tataggatac ggggtaggcg
     1501 gtgcttgaag ctagataggg agtcgccata agaagttgga agggctcata attgcgcaga
     1561 ggttgactga tgagaaggag tttgggagga gctgtcgaag tatgcaagca atacaaccgg
     1621 ttgcaaacat aatcatagta aggacagaat gccgacatac ataggttatt atgagtaaaa
     1681 caaccgattg ctatgctagg agatgttgca atgaggccga cgcttcaaga tatggcggat
     1741 attctcaatt tgtctaaatc aacagtgtca agagcgttgt ctggcgatcc tcgtgtagcc
     1801 caagctaccc gcgccagggt tgaatctctt gccgagagaa tcggctacac gcctaacccg
     1861 attgctcgag ggcttgcgac tcgccggacc aacacaattg gtcttgcggt tccatgtgca
     1921 cctcgctcgc tgtctgaccc gttttacttg gagtttctag ggcatgcggg caatgcggcc
     1981 atgaaatgcg gttatagctt attgctatcg gcggcagaag aggacggtgc agacactcat
     2041 aatgcgcatg tgcggctggc aagttccgct aggatcgacg ggatgatcct gactgaacca
     2101 aagatcaaag atgaacgaat tgcggcatta aaaaaggcgg gcattccctt tgtcttcctt
     2161 gggatgacgt ctgatccaga tgtgtcgtgg gttagttgtg acaacaagct tggcgctgct
     2221 gaagcggtag cgtggcttac ggggcttggg cataccagga tcgcgtgtct taccggtcct
     2281 gcggatcaaa cggcttccga tgctcgatat cgaggctatt gcgaggccct ggacgaggct
     2341 ggaattcccc ttgacctcaa tatcgtggct tcaggggatt tcactcaagc tggagggcat
     2401 gctgccatga gggaaatatt ggaacgtagg aagactgtgt cagccgtatt cgcctcaaat
     2461 gatgtcatgg cgctgggcgc tatgtgcgct cttcgcgaga gtggactgtc ggtgccagag
     2521 gacgtgtcgc ttgttggctt tgatgggatc gctttcgcgc aatactcgac gccgccattg
     2581 gcaactgtgc agcagcccat tgatgagttg ggaaatgctt cggttgaagt tctcataaaa
     2641 ctgatgtgtg gagataacgg gggacctata catcgggtga tgccggcgag gttccggcct
     2701 gctggctctg tggctgcgcc agggcgtgct tttgtgttgg aaagcccgga ttgaatgtag
     2761 tttgcgacgt accaagctgg aatccgccta caagattgga ggtgatgctg cgatcatttc
     2821 gccgagctca accctgtgag tgtcagtttt tgtaagcgag cacgagttag ccaagaaaac
     2881 tgcgaaagcg gagggattac agtgaagcga aagatgattg tgttgctcat ccttgcgctt
     2941 gccatctgca gtctgccagg ggccactgct gagaagaaac ttacagtgat tagcggatgg
     3001 tcagggcctg agatggatgc gttcatgccc gtgttgaagg catttgaaaa ggaaaccgga
     3061 atccgtgtgg actaccagat ttatcgagca gaagatttgg cggtcttgct tcctgctcag
     3121 tttaatgcca aaactacgcc tggggacgtc atatacatgt ggtcgtggtt tattaccaaa
     3181 catgcaaaag atggccatgt gcttgagatg accgggagcc ttaaggatgc cgacttcatt
     3241 ccaggggcgc ttgatgccgt caaggtcgat aacaagctgt ggggcggggt atataccggc
     3301 aaggtgaagc cggggttctg gtaccggaaa tcgtttttca aaaagcatgg actttccgag
     3361 ccgaaaacat gggatgaatt cgtcgcactc tgcgccaaac tccagaagat atccggcgta
     3421 aaggcaccaa ttgccagcgg aaatggggtt gggtggcctc tgtccgacat tacggagcat
     3481 ttcatcgcta catatgccgg tcctcaggtg acatacggtt tgatagacaa gaaagtcaag
     3541 tggactgatc ctgtggtgaa atccgtattt acagatagga ttgttccgct gctcaaagcg
     3601 ggttacttta gcgaaccgat tgagtggacc atggctctcg atctatggtg gaggggagac
     3661 tatggtcttt acttcatggg cagctggatc acgggcatgg ttgataaccc agatgatctg
     3721 ggcgttttct ctttaccagg atgcgaaggc atagtatttg cgcctgacta cgcttttgtg
     3781 ccagcctaca ccaaatatcc tgcagaggcc agaaaactcc tcgccttcct ctcaactaag
     3841 ggtcaggaga tacaggtttc cacaggtgga catattgcta catacaaggt tccgctcaac
     3901 gcctatcccc ctgttgaccg cggggtagcc tcccttctca agggagttgt tgctctgccc
     3961 gaccttgatg atactatcgg aggcgagtgg caggcggcgt tctgggatca gctcaaactg
     4021 ctctgggtga gcccaggcag ggtcaatgag gtccttgaga atctgcagcg gaaggccaat
     4081 taatcgtaat tggtagggct ccggagcaac caatcttgac aatgtggcgc tccggagcgc
     4141 ctttttctca gagcaagatg tcagttggga ggtggcgcta tgggtcagac gcgtggtaga
     4201 acgctttttt tcattccggc cctggcattg ttggctcttt ttgtcgttta tccagtagca
     4261 aacacgatat ggttgagttt tttcacgccg gaaggcgaat tcgcattctt caagaattat
     4321 gcggatgtcc tctctcagcc ggagatattc gacccgcgag ggttcgaacg gggcttccct
     4381 tatggggcgt tgattcacaa cgtcatatgg atagtgttac acttgcccct taccgtgata
     4441 ttggggttgg tattcgcagt cattctcaga gatatcaagg gtggagccat agtcaaatca
     4501 atagtctttc ttggaatggt cacccccatg gttgtgggag gcataatctt gagattcctt
     4561 tttgatggga atgtcggaat catcccatca gtactggccc ttttcggttt tgcgccgcga
     4621 actctcacag cctatccgga taccgctctc ttttccctta tagccgggtc gctttggctg
     4681 tggactccgt tcagcatggt gctttattcg gcaggcctgg agactatacc tgactttgtg
     4741 tatgaagccg cagtgataga cggtgcttcg ccttggagaa tatttttcaa tatcactgtg
     4801 cctttgctca aaccagtcac cggtgtcgtg gttaccatga cattcctgtg ggttctcaag
     4861 atctttgata tcgttttcgt tgctaccatg ggaggaccgg gaggggcgtc gaacgtgctg
     4921 gcgttgcaga tgtacatgta tgccttccgt gagttcaatc cgaatgccgc ggccgttgtg
     4981 gcgacgttcc tcatgattgt agctcttgtg gtgtccatac ctactttcaa ggctgcaggg
     5041 ggtgaagaat gatgagaacc gtaggatgca gcagtgggca caaagtgatt acgtatgtgg
     5101 ctgctgggct catagcgctc atatggctta tccctttttt gggagtcttc atggcctcga
     5161 taaggccttt gtcggagata aacaggggat ggtggaggtt tgattcgttc actccaacct
     5221 tcaaaaacta tgtaggcgct tggaatcatc ctgcagctcc gatttctgag ggcatgctta
     5281 actcactcaa ggtggcgatt cctgccacta tcatacctgt atttgtagcg tcgatcgcgg
     5341 catatggatt tgcgcgtttc aaattccggg ggagagatca gctcttcatt ctagtcgtag
     5401 cgctgatgac tttgccgcag cagatgatag cagtgccgat gttccagatg atgaacaagc
     5461 ttggcgcggt tgattcgtat tcaggcctaa ttctggtcca ctcggcttgg ggaattccat
     5521 gggctctcta cttcatgaga aattacttta ccacgctgcc cattgaagtg gaggaagctg
     5581 cgaaggtgga tggggcatca gactggagga tcttcttcca gatcatcatg cccatggcgc
     5641 ttcccgccat cgcctcggcg gcggtattgc agttcatgtg ggtatggagc gatttctttc
     5701 ttgcgttgat cctcatctat tcgcctgcga aactccttgc tacacaacgt gtgccgctgc
     5761 ttcgcgggat ctaccatgtg gactggggta ttctagctgc aggcagcata attgtgatgg
     5821 ctgttcctat tattgtcttc gccgtcatgc agagatatta catcagaggc atggtcggct
     5881 gggtgtcaaa atgacagatt gtgattacta ggctatggga gggcaatgta tgttccgcaa
     5941 agtcgatttc ttggaaaaat cccttgacga ccatgcgcca tatgcaggag aagacaggat
     6001 tgccgagatc agacgtcttg ctgaagacct cggcagcctg cgggtgcttc atgtaaattc
     6061 tacttcttac gggggaggtg tggccgaact tctttacacc atagtcccgc tgatgcgcga
     6121 cgccggactt gacgctgagt ggcatgtgat cgatggcgcg cctgaccagt tctttgatat
     6181 cacaaagaag atacacaact ccttgcaagg ggcaaacact ccgctgagcg atggcgagtg
     6241 gcg
//
LOCUS       DAOSAI010000104         6200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8051_length_6200_cov_4.399512, whole genome shotgun sequence.
ACCESSION   DAOSAI010000104 DAOSAI010000000
VERSION     DAOSAI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8051_length_6200_cov_4.399512"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLM83_04835"
     CDS             complement(<1..534)
                     /locus_tag="PLM83_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70767.1"
                     /translation="MPEQNALNRAAESADRAQPSLDEKGVARFTNVGALLLRIVVIVT
                     CIMFLLRTLSIQPAIIITIVPAIATVAGFNSIGGKHGYYVASILATLFDLILISATVF
                     ATGGIDSEAFLLYFLEIVVLSLNIGSARCVAAGMGCAGLYSLATWAPWANTPETSWIL
                     LYRCGLFMLVGCGVGRLS"
     gene            complement(576..1760)
                     /locus_tag="PLM83_04840"
     CDS             complement(576..1760)
                     /locus_tag="PLM83_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOK70768.1"
                     /translation="MNSVLAISLLMLSGVVAGRVVVGWGLSSVVGYLIAGIAIGQIWP
                     SIVEVITPVGVSFAAEVGLALIAFVVGGELEADRVRSLPRGVVGIAFMQAIGCILAVV
                     TACMWLDQPLGFGLVLGAIAAATAPSTTIMITRKYRSDGPLTNALLSVVAIDDAVCIL
                     LYAIAVAVARALPDGIMSQSPSGVFGVFLWELLGAVLVGVVVGIAMGFFIKYVRGADE
                     LVVVVVGAVLLAAGLASRMHVSPLISSLVLGATVSNLVVGSRRLFASADRFSPPIYVT
                     LFCLAGVGCSFNALSSSLGLFTAYVIARVAGKVIGAGIASGLAGVGRQIGSNIGLSLL
                     PQAGLAAGLTVAAGVALPDHYQMLASIIVPGMLLFEAVGPPLVAGSLRIAGEIHIGEP
                     SA"
     gene            complement(1788..2159)
                     /locus_tag="PLM83_04845"
     CDS             complement(1788..2159)
                     /locus_tag="PLM83_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70769.1"
                     /translation="MQVLFVVLNDTTLLDDLFSAFVRAGITGATCIESVGMGRTLSEI
                     DNSSIPIFAALRHHLNESRPYNRTIFALLPDDKVDEAISAVESVVGDLSMPGAGVLFT
                     VPVGKVVGFPKRHSSLDELGK"
     gene            2819..3472
                     /locus_tag="PLM83_04850"
     CDS             2819..3472
                     /locus_tag="PLM83_04850"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOK70770.1"
                     /translation="MFKQFIDISRELFTGIEVWPGDPEVQFGYVSSVSSDGAQVMELR
                     LGTHSGTHIDAPAHFIEGAMGIDEVPLSATIGPCLVIDLTNMHGPIDQAALEIHLPEE
                     YPPRLLLKTSHDSWQQAAYLNHEGARFLVESGVKLVGTEEMSIEQTDGDGRVHKTLLE
                     AGVAILEGIDLQGVAPGPYVLLALPLRLKGADGSPCRAVLGVLSEKWQPHVLCNSVP"
     gene            3743..3946
                     /locus_tag="PLM83_04855"
                     /pseudo
     CDS             3743..3946
                     /locus_tag="PLM83_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282068.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
     gene            complement(4151..4969)
                     /locus_tag="PLM83_04860"
     CDS             complement(4151..4969)
                     /locus_tag="PLM83_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOK70771.1"
                     /translation="MAYKAPAEIAKTVVDSSVTKANADFVTLVALGFLAGSFIAIAGA
                     TSHTVSLALGPWAGPGIIRFISGLIFTVGITMVIIGGAELFTGNSLMVAGLLEGRITI
                     VQLLRNWVIVYLSNFVGALFVAWLYYKTELWAAPGLEIAEGFVNTAIAKADIEFGTGF
                     ARGILCNWLVCMGVWMSYAADDVTGRVIPTAMAVAAFVMCGAEHSVANMFYMPVGFMI
                     RARISGLPALSMLPNLVSVTLGNVVGGGLLVATVYWVAYLRKSSSPKYVSPGRN"
     gene            5167..6003
                     /locus_tag="PLM83_04865"
     CDS             5167..6003
                     /locus_tag="PLM83_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOK70772.1"
                     /translation="MNARTNNNSKHIFTVALLAALILVANAAGASAATVYRVVAGDSL
                     FFIAQRYGVTVSAIRGANGLVGDMIYPGQTLTIPNGVSTTPPAQSGTSYTVKSGDSLF
                     LIARRHGTTVDALKAANGLYSEWIYPGQLLVIPSGTTISPDPNRGGTIPSRGAWSQSD
                     LTLLAQLVTAEAGGEPYEGQVAVAATVLNRISSGLYPNTIPEVIYQVVDGRYYQYSPV
                     LDGRINNAPSSTAIGATREALNGRDPSYGALGFYNPSKTSNYWVRSRTVTRVIGDHVF
                     FK"
BASE COUNT         1704 a         1751 c         1448 g         1297 t
ORIGIN      
        1 ggaaagccgc cctacgccac agccaacaag catgaaaagg ccacacctgt atagaagaat
       61 ccagctggtc tcgggcgtgt tggcccatgg agcccatgtg gccagcgagt aaaggcccgc
      121 acagcccatc cccgctgcta cgcatcgcgc agaaccgata ttgagagaca aaaccacaat
      181 ctcgagaaaa tacagaagga aagcctcgct gtcaatgccg cctgttgcaa agacagtagc
      241 ggaaatcaga ataagatcga atagtgtggc aagaatactt gccacataat acccgtgttt
      301 gccgcctata gaattgaaac cagcgaccgt cgctattgca ggcacaatgg ttatgataat
      361 tgctggttgg atcgacagag ttcttagcaa gaacatgatg catgttacta tcactacaat
      421 tctgagcaac agcgccccta cattcgtgaa tcgcgcaacc cctttttcgt caagcgaggg
      481 ttgcgcgcgg tcagcagatt cagcggctct attcagagca ttctgttctg gcatatgaca
      541 ccaccactca ctagcccttg aaagcgctaa acaccttatg cggaaggctc accaatatgg
      601 atttcaccgg caatcctcaa agaccctgct acaagaggag gacccacggc ctcaaaaagc
      661 aacattcccg gtactattat ggaggcaagc atttgatagt gatcagggag ggcaactcct
      721 gcggcaacag tcaagcccgc agcgagccca gcctggggaa gcaatgacag cccgatatta
      781 cttcctatct gtctcccaac cccggccaac cctgaagcga tcccagctcc gatcaccttc
      841 cctgccacgc gggcaattac atatgcggtg aaaagcccga gtgaagaaga gagcgcgttg
      901 aaactgcatc ccacccccgc aaggcaaaac agcgtcacat atatgggggg tgagaacctg
      961 tctgccgacg caaacaaccg cctgctccca accaccaggt tagatactgt tgccccaaga
     1021 acaagcgaac tgatcagtgg cgatacatgc atcctgcttg ccagtcccgc cgccagcaac
     1081 actgcaccga ccacaacgac cacgagctcg tccgcgcctc ttacgtattt aatgaagaat
     1141 cccatcgcaa tgccaacaac tacgcctacg agcaccgctc caagcagttc ccacagaaac
     1201 acgccgaaaa ccccagatgg agattgcgac ataatcccgt caggaagcgc cctagcaacc
     1261 gcaaccgcga ttgcgtagag aaggatgcat acagcatcgt caatggcaac gaccgaaagc
     1321 agagcatttg tgagaggacc gtcgctcctg tatttcctgg tgatcattat ggtggttgaa
     1381 ggtgcagttg ctgctgcgat ggcgcctaga accaagccaa agcctaatgg ctgatcgagc
     1441 cacatacatg ccgtcaccac agccaatatg cagccaatgg cctgcatgaa agctattcca
     1501 actacaccgc gaggaagcga acgaaccctg tctgcctcaa gctcgccacc gacaacaaaa
     1561 gcaataaggg ccaacccaac ctcagcagcg aatgatactc caacaggggt gatcacctcc
     1621 acaatgctag gccatatctg ccctatggca ataccggcga taaggtagcc caccaccgaa
     1681 gataggcccc agccgacaac cacccgcccg gccacaacgc cgctcaacat gagaagacta
     1741 atcgcaagaa cgctattcat atcaaacctc tccgccagct agggacctta tttcccgagt
     1801 tcgtcaaggc tcgaatgacg cttaggaaat ccaacaacct ttccaacagg cactgtgaaa
     1861 aggactccag cgccaggcat gctaagatct ccgacaacac tttccacagc tgaaattgcc
     1921 tcgtccacct tatcatcagg caacagagca aagatagtgc ggttatatgg cctgctttcg
     1981 ttcaggtggt gcctaagggc agcaaagata ggtattgatg agttgtcgat ttcagacaaa
     2041 gtacgaccca ttcccacgct ttctatgcaa gtagcccctg ttatacctgc cctgacgaaa
     2101 gccgaaaaaa ggtcatccag gagcgtcgta tcattcaaga caacaaatag aacctgcaag
     2161 caagttccct ccagtaattc ctcagataga ggcaagtcca ttgcttttac tataacctac
     2221 tatgtattgg gtgacaagat actgtatagt gctccaatat cagcaattac ccaaatcacc
     2281 cttggattcc ttcattaagt tatcatagcc cttagcttct ccaatatcaa ctgatgcgtc
     2341 gtcggtttat gtcggaaata cgatctccaa agaaaaacct tgtgggaaaa cttgggagct
     2401 tactgccata ctcgccatat tccgcgccag acacaccgtt tcctctatgt tggtcaattt
     2461 cgtgagctcg aaaacaagac gaggacggcc ttcaccagcg tccgtgcatc gcaaaaagcg
     2521 gaccggaaac ttccctgaag caaaaaggct agatacagct tccataattg tcgcccccac
     2581 acgcagaata ttcggggaga aagcgcattg atactccaca caatctgacg agtctttcgg
     2641 ctatgggaca cactgtacaa caggtcagct gtggcacgcg ctttttccaa ccttttcgat
     2701 atccaggggc gcgcaagagc aaatcttctt gcttaatatc agagggagag acatcaatat
     2761 gcagaagtac cggttaacac acatatccaa aatgcgatca tcgcttgggg gcgattggat
     2821 gttcaagcaa ttcatcgata tatctaggga gcttttcact ggcatagaag tctggccggg
     2881 cgacccagag gtccaattcg gatatgtctc atctgtatcc agcgatggcg cccaagtcat
     2941 ggaactgcgt ctggggacac actcaggaac tcacatagac gcccctgccc acttcatcga
     3001 aggagctatg gggatcgatg aagttccact ttctgctact atcgggccgt gtcttgtaat
     3061 cgacctcaca aacatgcacg gaccgatcga tcaagctgct ctagagattc atctgcctga
     3121 agagtatcct ccgcgactgc tcctgaagac gtcgcacgat tcctggcaac aagcagcata
     3181 cttgaaccac gagggagcca gattcctcgt cgaatccgga gtaaaactgg taggaacgga
     3241 agagatgtca atcgagcaaa ccgatggaga cggtcgtgtt cacaaaactc tgctggaagc
     3301 gggcgtggca atccttgaag gaatagatct gcaaggagtg gcacccggtc catacgtcct
     3361 ccttgcacta cccctgcgtc ttaagggagc cgacggatct ccatgccgcg ccgttctggg
     3421 ggttctttca gagaaatggc agccacatgt tttatgcaat tcagttccat aatttttcaa
     3481 agacagcagg atgcccaccc tggagcttag ttggaacaaa ataggtttct agtagcgtag
     3541 gcatcacatt gatacaagcc gttcttactg tagaatggtt acccgcattc tggatcttat
     3601 tctctccgct cgccaacttc gctcgagcaa aatgtgagtt cctcatgtga cgtaaagttc
     3661 tagtatacgt tcgacaacga cggtcacact agaaacgcag tcgcatgcag caagaatacg
     3721 cgtgagaagg gaggagtatt ttatggcgag acagagcaga agcaatagga ttctcaaccc
     3781 acaggctcgt caggctcttg accagatgaa atatgagata gccagtcagg tgggggtcga
     3841 ttataacgct tatggcgggt atctcggaga cattcccgcc cgcgaggccg gaaggatcgg
     3901 cggccagatg gttaagcaca tgatcgcagc ggcggagcag agcctcatta gtcaaaccgt
     3961 cgccggcact caggccagtt tcgcacaggc tctgaggccc caagtttctg gcaccttccc
     4021 aacagaaccc acatcaacct acacgactca gacaaacctt aacgtgccaa aatcataaaa
     4081 gcttgcccac gcaagcgccg gcacgtaaag acggcgcccg gtcgctctgg ccggacgccg
     4141 tctcattgcc tcagttccgg ccagggctta catatttagg cgatgagctc ttacgaaggt
     4201 atgcgaccca gtacacagtc gcaaccaata agcctcctcc cactacgttc cctaatgtca
     4261 cagaaaccag atttggcaac atggagaggg caggcaatcc actgatacgc gcgcgaatca
     4321 tgaaacctac cggcatgtag aacatgttcg ccaccgaatg ctcagcgcca cacatcacga
     4381 aagcggcaac tgccatcgcc gttggtatga ctctgccggt cacatcgtca gcagcatagg
     4441 acatccaaac acccatacat accagccaat tgcataggat tccccttgca aaccccgttc
     4501 caaattcaat atcggccttt gctatggctg tgttaacaaa gccttcggcg atttccagtc
     4561 ccggagcagc ccaaagctct gtcttgtaat agagccatgc aacaaagagg gcgccaacga
     4621 aattgctcag gtagactatg acccagtttc ggaggagttg aacaatggta atgcgccctt
     4681 ccaaaagccc tgcaaccatc agcgagttcc cggtgaaaag ttcagctcca cctattatga
     4741 ccattgttat cccaactgtg aatatgaggc ctgatatgaa gcgaattatt cccggtccgg
     4801 cccagggccc gagagccaaa gagacagtat gggatgtcgc cccagctatc gctatgaaac
     4861 ttccagcaag aaatcccagc gccaccaggg ttacaaaatc tgcgttcgct ttggtcactg
     4921 aagagtcgac aacagtcttc gctatttccg caggcgcctt gtacgccatt tccagcacca
     4981 tcctttgtcc atgaaagtgg ctgtacgcca actatttcac tgaaatgcaa cattgtcaat
     5041 gtcgcaccaa gtcgcttttc ccatcacttc ccaacgggaa attccccttt ctttgacgaa
     5101 tacatcgtta gtctggaaga tcgccccctt ccaaaccagg tttatcagag aaaggagtta
     5161 caactattga acgccagaac aaacaacaac agcaaacaca tcttcacagt tgcacttctt
     5221 gcagctctaa tcctggtcgc aaacgctgcc ggggcatccg ccgccaccgt atacagagtg
     5281 gtcgcgggcg attcactgtt tttcattgcc caacgatacg gcgtaaccgt cagtgccatt
     5341 cgtggagcaa atggattggt aggcgacatg atataccctg ggcaaacact gacaatacca
     5401 aatggggtct caaccactcc gccagcacaa tcgggcacta gttacacagt gaaaagcgga
     5461 gattctctgt tcctgatagc gcggcgacat ggaacaacgg tagacgccct aaaggctgcc
     5521 aatgggctct atagtgaatg gatctacccc gggcaactac tggtaattcc ttctggaaca
     5581 acaatatcgc ctgatccaaa tcgcggcggg actatccctt caaggggagc ctggagccaa
     5641 tccgacctga ctctccttgc acagttggtt accgcagaag cagggggcga accctatgag
     5701 gggcaggttg ccgttgcagc cactgttcta aatagaataa gcagcggcct atatcccaac
     5761 actattccag aggtcatcta ccaggtggtg gatggtcgct attaccagta cagtcctgtg
     5821 ctggacggac ggatcaataa tgcaccctcg tccacagcta taggcgcaac aagagaggcc
     5881 cttaacggac gtgacccatc ttatggggct ctgggctttt ataacccatc caagacttct
     5941 aattactggg tgcgttctcg caccgtaaca cgggtaatag gcgaccacgt attcttcaag
     6001 taaggggcgc aatcatctgt gccatacaaa cgtaatggcg catcaattcc ccggagcgcg
     6061 atctccgggg aattggcttg ccaccgatga tgaaagagtc cccatcccct cttatgtaag
     6121 cggctcgagc agctattgaa ttatccaaca gaacaatgta caatcgtccg agggagggac
     6181 agaccgtgcc aatctacgag
//
LOCUS       DAOSAI010000105         6176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8089_length_6176_cov_3.661820, whole genome shotgun sequence.
ACCESSION   DAOSAI010000105 DAOSAI010000000
VERSION     DAOSAI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8089_length_6176_cov_3.661820"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /gene="purQ"
                     /locus_tag="PLM83_04870"
     CDS             <1..662
                     /gene="purQ"
                     /locus_tag="PLM83_04870"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOK70773.1"
                     /translation="NAIEAVGFDAEFIWHRDTSVAGYDCLVLPGGYSYGDYLRPGAIA
                     RFSPVMDSVVEYARDGRPVIGICNGFQILLEAGLLPGAMQRNVGTRFICDRVRVRIES
                     TRTPFTSGCREGDVLTLPIAHADGNYYADPQTLAELEERGQVVFRYCNELGDATPEAN
                     PNGSLNNIAGVSNEAGNVVGMMPHPERACEAILGGTDGRRIFESVAEWVRGGVRVGQD
                     G"
     gene            646..2913
                     /gene="purL"
                     /locus_tag="PLM83_04875"
     CDS             646..2913
                     /gene="purL"
                     /locus_tag="PLM83_04875"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOK70774.1"
                     /translation="MAKTDSLKESIWRSMGLSDEEYQMIQDLLGREPNYVELGMFSVM
                     WSEHCSYKNSRAVLKGLPTSGPQVLQGPGENAGIVDVGDGLAVAFKIESHNHPSAVEP
                     YEGAATGVGGIIRDIFTMGARPIASLNSLWLGDLQSNKSRMLFDGIVSGISGYGNAVG
                     VPTVGGQTVFHQSYENNPLVNAMCVGVMRQDEIKRSAASGVGNSVMLIGSTTGRDGIH
                     GATFASADLTEEAVEKKSAVQVGDPFREKLLLEACLELVARGCVLGIQDMGAAGLTCS
                     SCEMAARGNVGMVIDIDKVPRREDGMTPYEVMLSESQERMLLVPVPGKEDEVKNVLEK
                     WDLLAEEIGKVTDDGFITLLEGGEIVAQVPAKALAENAPLYHRESVRPNYLDEVALYD
                     VGALEAPKDLGGALLQLLGSPNLASKKWVYRQYDQQVGAGTVVGPGSDAAVVRLPGTS
                     RGIALTCDCNSRYCYVNPRLGAAHAVAEAARNLACTGARPLAATNCQNYGNPYKPEVF
                     WQFKEGIEGMAEACIALNTPITGGNVSFYNEDGEMAIRPTPVVGMVGVIDDVEDCVAM
                     GFSQEGDVIVLLGTTRDEIGASEYLGTVHGVEAGQVPELDLDVERRLCELLLAAADRG
                     ITRSVHDLSDGGLGVALAECCIAGSCGARVRLEANIAPDRFLFSESAGRAIISVDESD
                     LGALLDLADEMGVAAKAIGEVGGESLDITCVESACVPAQGDDRRAQEAEVVIALPIAK
                     LAQVYEEAIPCAMER"
     gene            2895..5372
                     /gene="purF"
                     /locus_tag="PLM83_04880"
     CDS             2895..5372
                     /gene="purF"
                     /locus_tag="PLM83_04880"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOK70775.1"
                     /translation="MRHGALRAQEADKLKEECGVFGACFREDAAVAVYYGLVSLQHRG
                     QESAGIAVYDGSRIRVVKGAGLATEVFEGDAFKSLPGKTGIGHVRHGVCQDNDVSGAQ
                     PFVFRHGGGMMAVAHNGSLTNGRVLRERLGRLGVVFQSESDSEVIGSLIARYFSLGMV
                     GAIERAMEELEGAYSFVVMVDDTIYAVRDPYGFRPLIAGSGPAGWAVSSESCALDAVG
                     ITEKRDVGRGEIVVISHDGMKRIATRASRRSCPSATCIFEYIYFARPDSIIEGIGVAA
                     AREAFGAMLAREARFDADVVVAVPESGVTAALGYSDESGIPYRQGLTKNRYMGRTFIR
                     NSQMERELGVRLKLNAVERSCAGKRVVLVDDSIVRGTTGARLVDLMRKAGATRVDMAV
                     SSPPVKFPCTYGIDTASRRDLVAAVKSEDEIREMIGADALHYLSIDGMLEAIGGLASA
                     RDTGEPLEFCTACLDGRYPDAISCHGKCRPPKSLSYADSGVDIDEGERAVQLIKPAVK
                     STFTPGVIGDIGGFGGLFKPDFAGMKEPVLVSGTDGVGTKLKIAFAADRHDTIGIDAV
                     AMCVNDVLVSGASPLFFLDYIGCGRLDAGQIAEIVGGVAEGCRQAGCALIGGETAEMP
                     GFYPDGEYDIAGFAVGAVDRGRIIDGSKIRPGDRVIGLASSGLHSNGYSLARKALCEV
                     GGFLLSDIPEGMDRPLGDEMLEPTRIYVRPVLALIERVEVLGMSHITGGGLVQNPPRI
                     LPHGASMALSVDTWRRPPIFDVISRTAGVAADEMFRVFNMGIGFLVVVRPDKAREAVN
                     ILVDQGEDARIIGEIIPGDGRVEFQGV"
     gene            5376..5939
                     /gene="purN"
                     /locus_tag="PLM83_04885"
     CDS             5376..5939
                     /gene="purN"
                     /locus_tag="PLM83_04885"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOK70776.1"
                     /translation="MRLVVLVSGRGSNLQAIFDAIEGGRLDAEVAAVISDSPDAYALK
                     RAEKAHVPGFCIERRDHSSRADFEAALAACAEAARPDMVVLAGFMRVLGPVFLDRFLG
                     RVINIHPSLLPSFPGLEAQRQALEAGVRYTGCTVHFVDYGVDSGPIIDQRVVPVLAGD
                     DVERLSARILEQEHDLLVDVIRRLGCK"
     gene            5970..>6176
                     /locus_tag="PLM83_04890"
     CDS             5970..>6176
                     /locus_tag="PLM83_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70777.1"
                     /translation="MARGRALISVYDKEGIEEFARSLAELGFEIISTGGTAALLRKAG
                     VDVRDVSEITGFPEVLDGRVKTLHP"
BASE COUNT         1274 a         1481 c         2070 g         1351 t
ORIGIN      
        1 gaaacgcaat cgaggctgtg ggctttgatg ccgaattcat ctggcatcga gacacgtcgg
       61 tcgcaggata cgattgcctg gtgcttcccg ggggttactc atacggagat tatctgcgac
      121 cgggcgcaat cgcgcgtttc tcgccggtca tggattcggt ggtggaatat gcacgcgatg
      181 ggaggccggt gatcggaata tgcaatggtt ttcagattct cctcgaggca ggattgttgc
      241 ctggtgccat gcagagaaat gtgggaacta ggttcatctg tgatcgtgta agggtccgca
      301 tcgagtcgac taggactcca tttacatcgg gctgtagaga gggcgacgtg ttgacactgc
      361 ccatagccca tgccgacggc aattactacg ccgatcctca aacccttgcc gagcttgaag
      421 aacgtggaca ggtcgtcttc agatactgca atgagctggg cgatgccact cctgaggcta
      481 atcctaacgg aagcctcaac aacatagccg gagtgagcaa cgaggctgga aacgtagtgg
      541 gaatgatgcc tcatcctgag agggcatgcg aggcgatctt gggcgggact gatggcaggc
      601 ggatattcga atcagtcgca gaatgggtgc gtgggggtgt aagggttggc caagacggat
      661 agccttaaag agtctatttg gcgatcgatg ggtttgtcgg atgaggagta tcagatgatt
      721 caggacctct tgggaagaga acctaattat gtggagttgg gaatgttctc cgtaatgtgg
      781 tctgaacact gctcctacaa gaattccagg gccgtattga agggccttcc tacttcaggc
      841 ccccaagtcc ttcagggtcc aggggagaat gcgggtatag tcgatgtagg tgatggactc
      901 gctgtagcct ttaagattga aagccacaat catccgtccg cggtggaacc ctacgaaggg
      961 gctgctaccg gggtgggggg tataataagg gacatattca ccatgggggc taggccgata
     1021 gcctccctca actcgctgtg gctcggcgat ctgcagtcta ataagtctcg catgcttttc
     1081 gatggcatcg tttcgggcat atccgggtat ggaaatgccg taggggttcc cacagtgggc
     1141 gggcagacag tattccatca gtcctacgag aataatcccc ttgtcaacgc catgtgcgtc
     1201 ggcgtgatga gacaggatga gataaagcgc agcgcggcca gcggtgtggg caactcggtg
     1261 atgctaatcg gatctaccac gggccgagat gggattcacg gagctacctt cgcatctgcc
     1321 gacctaactg aagaggccgt ggaaaagaag tcggcggtcc aagtaggcga cccgttcagg
     1381 gagaagctcc ttctcgaggc atgcctggag ttggttgccc gcggctgtgt cctgggaatc
     1441 caagacatgg gggcggcagg acttacatgt tcctcgtgtg aaatggccgc gcgaggcaat
     1501 gtgggtatgg tcatcgatat cgataaagtc ccgcgccgcg aagatgggat gactccatac
     1561 gaagtgatgc tctccgagtc tcaggaacgc atgctcttag tgcctgtgcc gggcaaagaa
     1621 gacgaagtca agaacgtgct ggagaagtgg gacctgctgg ctgaggagat aggcaaggta
     1681 acggatgatg gattcataac tctcttggaa ggcggagaga tagtagcgca ggttcccgcc
     1741 aaggcgctcg ctgaaaacgc accgctgtac catagggagt ccgtgcgccc caattacctc
     1801 gatgaagtgg cattgtatga tgtcggtgct ttggaggccc cgaaggatct gggcggagct
     1861 ctgcttcagc ttctgggctc accaaatttg gcgagcaaga aatgggtgta caggcagtat
     1921 gaccagcagg ttggcgctgg tacagtagtc gggcccggat cggatgccgc agtagtgcgt
     1981 cttcccggca cgtcccgagg catagctctc acctgcgact gcaactccag atactgttat
     2041 gtcaatcctc gtctcggcgc tgcccacgca gttgccgagg ctgctcggaa cttggcctgt
     2101 accggggccc gtccactggc tgcaactaac tgccagaact acggcaaccc gtataagccc
     2161 gaagttttct ggcagttcaa agagggcatt gaagggatgg cggaggcatg catcgctctg
     2221 aatacgccga ttaccggggg caacgtgagt ttctataacg aagacggcga aatggcgata
     2281 cgtcccactc ctgttgttgg catggtaggc gtcattgatg atgtcgagga ttgcgtagca
     2341 atgggattta gccaagaagg ggacgtgatt gttctcctgg gaacgacgcg cgacgagata
     2401 ggagcgtcgg agtatcttgg aacggtccac ggagtagagg ctggtcaagt gcctgaactt
     2461 gatcttgacg tcgagaggcg tctgtgtgag ctccttctgg cggcggcgga cagggggatt
     2521 accaggtctg tccatgatct ttcagatggt ggcctagggg ttgccctggc cgaatgctgc
     2581 atcgcagggt cttgtggcgc tagggtgagg ctggaggcga acattgctcc cgataggttc
     2641 ctattcagtg aaagcgctgg aagagccatt atcagtgtgg atgagagcga tctgggggcc
     2701 ctgctcgacc tggcggacga gatgggcgtt gccgcgaaag ccataggcga ggtgggcggt
     2761 gagtctctgg atatcacgtg cgttgaatct gcttgtgttc ctgcgcaagg cgatgaccgg
     2821 cgggcccagg aggcggaagt tgtcatcgct ctgcccatcg ccaaattggc ccaggtttac
     2881 gaggaggcga taccgtgcgc catggagcgc tgagagcgca ggaagcggac aaactgaagg
     2941 aagaatgcgg cgtatttggt gcctgttttc gtgaagatgc cgcggttgca gtctactatg
     3001 gactcgtttc ccttcagcac agggggcagg agagtgcagg gattgccgtg tacgatggat
     3061 cccgtatccg ggttgtgaaa ggagctggcc ttgccacaga ggttttcgaa ggcgacgcgt
     3121 tcaagagcct tccaggaaag acgggcatag gccatgtgag gcatggcgtg tgccaggaca
     3181 acgacgtctc aggcgcgcag ccctttgtct ttcgtcacgg aggcgggatg atggctgttg
     3241 cccataacgg aagcctcacc aacgggcgtg tattgcggga gaggctggga aggttgggtg
     3301 tggtctttca aagcgaatct gattcggaag tgataggttc cttgatagcc cgctactttt
     3361 ccctgggcat ggtgggcgcg atcgaacgtg ctatggagga attggaaggg gcctactcct
     3421 tcgtggtgat ggtcgatgac accatatatg cggtccgcga tccctatggg ttcaggccgt
     3481 tgattgcagg gtcaggccct gcaggatggg ctgtgtcttc cgagtcgtgt gctctcgatg
     3541 ccgttgggat aaccgagaag cgcgatgtcg gcagagggga gatcgtagtc atctcccatg
     3601 acggaatgaa acgtatcgcc actcgagcct ctaggaggag ctgtccctct gccacctgca
     3661 tatttgagta catctacttc gcccgacctg attccatcat tgaaggtatt ggcgttgccg
     3721 cagcccgcga ggcctttggc gccatgttgg cccgggaagc ccggttcgat gccgatgtcg
     3781 tcgttgcagt tccagagtca ggcgtcactg cggcactggg atattcggat gagagcggaa
     3841 tcccgtaccg ccaagggctt acgaagaacc gctatatggg gcgaaccttc atcagaaact
     3901 cccagatgga acgggagttg ggcgtgaggc tcaagctgaa cgcggtggaa agatcgtgcg
     3961 ctgggaagcg cgtggtgttg gtggatgact cgatagtgcg gggcactacc ggagcccgcc
     4021 ttgtcgactt gatgcggaaa gccggcgcca cgcgggtaga catggcggtc tcgtcaccgc
     4081 ccgtcaagtt cccgtgcacg tatgggattg acacggcgag caggcgggat cttgtggctg
     4141 ctgttaagag tgaagacgag atccgcgaga tgataggcgc cgatgcgctg cattatctgt
     4201 ccatcgatgg aatgctggaa gccattggtg gcctggcgtc ggcgcgcgat actggtgagc
     4261 cgttggaatt ctgtaccgcg tgcctggatg ggcgatatcc cgacgccatc tcctgtcatg
     4321 ggaaatgcag gcccccaaag agcttgtcgt atgctgattc aggtgtcgat atcgatgagg
     4381 gagagcgggc ggtgcagctc atcaagcccg cagtgaagag cacgttcacc cctggggtca
     4441 tcggggatat cggaggtttt ggaggcctct tcaaacctga ttttgcaggt atgaaggagc
     4501 ctgttctggt ttctggcacc gacggggtgg gaacgaagct aaagatcgcc ttcgcagctg
     4561 accgccatga caccattggc atcgatgcgg tggcgatgtg tgtcaacgat gttctggtgt
     4621 cgggggctag ccccctgttc ttcctcgact acatcggctg tggaaggctg gatgcggggc
     4681 agatagccga aattgtcggg ggggttgctg aaggctgcag gcaggcggga tgcgccctca
     4741 taggcggcga gacggccgag atgccgggat tctacccgga tggagaatac gacatagccg
     4801 ggtttgccgt tggcgctgtg gaccgcggac ggataatcga cgggtcgaag attcgtcctg
     4861 gggacagagt gatcggtttg gcttcgtcgg gactccactc caacgggtat tctctcgcgc
     4921 gcaaggcctt atgcgaagta ggagggttcc ttctttccga tatcccggaa ggtatggacc
     4981 gtccgctagg cgatgagatg ctcgagccca ccaggatata tgtcaggcca gtacttgctt
     5041 tgatcgagcg tgtggaggtg cttggcatgt cccatataac cggaggcggg cttgttcaga
     5101 atccacctag gattctgcct cacggagcct caatggcgct gtcggtcgac acgtggcgtc
     5161 ggcctccgat attcgatgtc ataagccgga cggcaggggt cgcggcggac gagatgttta
     5221 gggttttcaa tatggggatc ggatttctag tggtagtgcg tcccgacaaa gcgcgggaag
     5281 cagtcaacat ccttgtggat cagggcgagg atgcccggat tataggggag ataattcccg
     5341 gagacgggcg agtggaattt cagggggtgt aagaattgcg cttggtcgtg ctggtttcgg
     5401 ggaggggctc caacctccag gccatcttcg atgcgatcga ggggggaagg ctggatgcgg
     5461 aggtagcggc tgtgatctcc gactcgccag acgcatatgc cctgaagcgc gccgagaagg
     5521 cgcatgttcc cgggttctgc attgagaggc gagatcattc gtcgcgggcg gattttgagg
     5581 cggccttggc ggcatgtgca gaggccgccc gacctgacat ggtagtcctt gcaggcttca
     5641 tgagagtgct cggacccgtg ttcctagaca ggtttttggg cagggtgatc aacatccacc
     5701 cgtcattgtt gccgtcattt ccgggccttg aggctcagcg tcaggctttg gaggccggcg
     5761 tgcgctacac aggctgtaca gtccatttcg tcgattacgg ggtggattca ggaccgatca
     5821 tcgatcagag agtggtgccg gtattggccg gtgacgatgt ggagaggctt tcagcgagga
     5881 tactcgagca ggaacatgac ctactggtcg atgtcatcag acgccttgga tgcaaatagc
     5941 gtcagggcgc aagaaaggag agatgtccgg tggcgagggg ccgggcgttg ataagcgttt
     6001 acgacaagga aggaattgaa gagtttgcta ggagcctggc ggagctgggg ttcgagataa
     6061 tctctaccgg gggcactgcc gcgctgctcc gcaaggccgg ggtggatgtg agggatgtct
     6121 cagaaatcac ggggtttccg gaggtccttg acgggcgtgt caagactctt catcca
//
LOCUS       DAOSAI010000106         6140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8143_length_6140_cov_3.571898, whole genome shotgun sequence.
ACCESSION   DAOSAI010000106 DAOSAI010000000
VERSION     DAOSAI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8143_length_6140_cov_3.571898"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..753)
                     /locus_tag="PLM83_04895"
     CDS             complement(136..753)
                     /locus_tag="PLM83_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOK70778.1"
                     /translation="MREALESGNAPKVKALAEQALAEGRSATEILNEGLIAAMSVVGV
                     KFKNNEIYVPEVMVAARAMTAGLNVIRPVLVETGAPSEGKVVIGTVKGDLHDIGKNLV
                     KMMFEGAGFEVVDLGVDVSADAFVDAVRNERPDILGLSALLTTTMPAMKTTIDALEEA
                     GIRDQVKVIVGGAPVTDHYAREIKADGYAADASSAVDLGRRLISA"
     gene            complement(863..2140)
                     /locus_tag="PLM83_04900"
     CDS             complement(863..2140)
                     /locus_tag="PLM83_04900"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOK70779.1"
                     /translation="MTCLDDILADGALTRVLRSFKEATGIAARIVKATGAPAIASRTW
                     EDCAFCRLVRSSEEGRNRCSRSYAYAARQAAYLGDLYVFQCHAGLVCWAAPLVSDGTL
                     LGAVICGQVIMWEPDEYLIRDSVERLRDLDIPLPQLEAAITQLEQVTTRRVQAAAELL
                     FAVSTYIVRSEDLALEQRNEIYRQQRLLGEAIQERKRIEEQFSIRGLYSPQQERELLS
                     AVRSGQRTEAKRMLNEILADIFLSQPDRPDIIKARLLELAVFLARAAVEAGADPERIL
                     GLNYQSIGDLSRCETFEEVCFWIVRVMDEFMDAVQDTPDLPSGQVQEAIRFMQSNLAS
                     KLSLADIASSVHISPSHLSHLFKEETGMSVMDCLTALRISEAKHLLAMPQLTVAQVSA
                     AVGFQDPAHFSRSFKRMEGISPTAYRQSMISAG"
     gene            complement(2137..2277)
                     /locus_tag="PLM83_04905"
     CDS             complement(2137..2277)
                     /locus_tag="PLM83_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70780.1"
                     /translation="MWRTRFASIFAYNHALVFRIPAILLSFKEIAREPQNVELRNGGL
                     SK"
     gene            2413..3267
                     /locus_tag="PLM83_04910"
     CDS             2413..3267
                     /locus_tag="PLM83_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK70781.1"
                     /translation="MAGLVETLQDMGLSLNEAKAYQALLRYGAANGYEVSKRSGITRS
                     VVYGVLDRLLEKGFVLQVDSDPVMYAPLPPSQLLKEYRELYDRNLDNLERSLKQMESG
                     VSEESYILGVAGYEDTIRKARELIRGTKHEVVVSAWGSDCVPLKEELEAAEGAGRTVI
                     IFCHTKVPFSVGTIYEYGLAEDIIKEKWPNRRIIVATDCRNVLVGDIRHDENLGIVTT
                     NPMIVQMAVEHVILDILHLAQLKAGIGDLAEQIQSGADYLRVIEDQHAKLDLGGAVLP
                     TPVPLPSD"
     gene            3426..4577
                     /locus_tag="PLM83_04915"
     CDS             3426..4577
                     /locus_tag="PLM83_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK70782.1"
                     /translation="MNCTTMDIPDDLVARAKRDLADVWEGRSIEPVILLAPEDPALPD
                     YSVAERMEDPEKDLEYQLASAHWDLACLRAGFCNIPSIWPYMGVGVIPSAYGCEIRIT
                     PGEDPWTIPITDDPDDVYRLKKPDLARSGLAAKVLDRIGYFSEMTGGTVPIRLTDVQS
                     PMDTATQILNYEELLIAMYTAPDAVHFLLEMVTDSLIEFVRMQEARAVNFFGHTHVNL
                     WRPRGIYVSDDVTAVVSPQHYREFMKAPNERLSRAFGGISIHCCRGYEQNLMEVANLE
                     GFMSFDADSHYNSIDSIGRALAAVDGCPNWGGAGEEKPLREDERRSRGIWHAWAEDAD
                     EAIYRVRAARRWGFGMILMLKCGGVAEGVEAAREIHRATEGGVWLTDED"
     gene            5346..5552
                     /locus_tag="PLM83_04920"
     CDS             5346..5552
                     /locus_tag="PLM83_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70783.1"
                     /translation="MIKKKIRKDDGRYLIYYSFPLNDGKGGDEYLAIRGDDEGGVTDS
                     VGDAVEPCAQAMGHNCNPQAGKDV"
     gene            5485..>6140
                     /gene="galT"
                     /locus_tag="PLM83_04925"
     CDS             5485..>6140
                     /gene="galT"
                     /locus_tag="PLM83_04925"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOK70784.1"
                     /translation="MRWNPVLKQWVITATHRQERTYKPPEDFCPLCPTRDTSFPTEVP
                     DYSYEIVVFENKFPSFRHVPPEPAVEGTGVLRVQPAQGICEVVLYTDEHHTTFADLDI
                     DHITNLIAVWTDRYSELGSIDYVDYVLIFENKGDAVGVTLHHPHGQIYAFPYIPPIPA
                     KELESAEEYRDATGRCLFCDILEQELDDGRRVIAGNSHFAAVVPFYARYPYEVHVTSK
                     "
BASE COUNT         1387 a         1570 c         1774 g         1409 t
ORIGIN      
        1 atggagcacg ggattgaact cgttctgctt gagtgaggag ccagtcagct ttgctccggg
       61 gtagcccaag agcggggcga cgagccttcg tttgcctgac attgccagtt ctatcagttt
      121 catgatcgtg tccccctatg cggagatcag ccggcggccg agatccactg ccgagcttgc
      181 gtctgctgcg taaccatctg cttttatctc cctggcatag tggtctgtga ccggcgcgcc
      241 gcccacgatc acctttacct ggtccctgat ccccgcttcc tccagcgcat cgatcgtggt
      301 cttcatagct ggcatagtcg ttgtcagcag ggctgacaac cctagtatat caggccgttc
      361 gtttctgact gcgtccacaa atgcgtccgc ggaaacgtcg accccaagat ctacgacttc
      421 gaagcctgct ccctcaaaca tcatcttgac cagatttttg ccgatgtcat gaaggtctcc
      481 tttgactgta cctattacga ctttgccctc agacggcgct ccggtctcaa cgagcacggg
      541 cctgatcacg ttgagacctg cggtcatggc tctggcagcc accataactt caggaacgta
      601 aatctcgttg ttcttgaact tgacgccaac cacactcatg gcggcgatca atccttcgtt
      661 taggatctcg gttgcgcttc ggccttctgc aagggcctgc tctgcaaggg cctttacctt
      721 gggcgcattt ccactttcca aagcttctct catttctaag taatcatgca atgccctaac
      781 ccccttgtgt cagatagttc accaactccc attttagagg aatggcaaat gaagtccatg
      841 gacggtcttg tggtttcatg gattatcctg ctgaaatcat gctttggcga taggctgttg
      901 gcgagattcc ttccatccgc ttgaacgagc ggctgaaatg ggcggggtct tgaaacccca
      961 ctgcggcaga aacctgtgcc actgtgagct gcggcatggc caggaggtgt ttggcttcgc
     1021 taatgcggag ggctgtgagg cagtccatta ccgacatgcc tgtttcctcc ttaaagagat
     1081 ggctcagatg ggaggggctg atatggactg atgatgctat gtcggccaaa gacagtttgg
     1141 aagcgaggtt gctttgcatg aacctaatgg cctcctgtac ttgccctgag ggcaaatcag
     1201 gagtatcttg gacggcatcc atgaactcgt ccattacccg cacgatccag aaacacacct
     1261 cttcgaaagt ctcgcaacgg gacagatctc cgatggactg gtagttgagg ccgagtattc
     1321 gttcggggtc ggcgcctgct tccactgcgg cgcgggcgag gaagacggca agttcgagga
     1381 gacgggcttt gatgatgtcc gggcggtcag gttgactcag gaaaatatcg gccaggatct
     1441 cattgagcat ccgctttgcc tccgttcgct gcccgcttcg gactgccgat aggagctccc
     1501 tttcttgctg aggcgaatag aggccgcgga tggaaaactg ctcttcaatg cgcttgcgtt
     1561 cctggatggc ttcgccgagg agccgctgtt gcctgtatat ctcgtttctc tgttcgagtg
     1621 ctaggtcctc ggagcgcact atataagtgg atactgcgaa gagcagttcc gcagcggcct
     1681 ggacgcggcg ggttgttacc tgctcaagct gggtaatggc ggcttcaagt tgaggaagag
     1741 gaatatcaag atcgcggagg cgttcgacgg aatcgcgaat cagatattcg tctggctccc
     1801 acatgatgac ctgcccgcag atgaccgcgc ctagcagggt accgtcgctc accagcggcg
     1861 cggcccagca gacgagcccg gcatggcact ggaagacata gagatctccg aggtatgcgg
     1921 cttgccttgc ggcatatgca tacgacctcg aacagcgatt acggccttcc tcggaggaac
     1981 ggaccaggcg gcagaaggcg cagtcttccc atgtccggga ggctatggct ggcgccccgg
     2041 tggcttttac gatgcgcgcc gctatccccg tggcttcttt gaaggatcgg aggacgcggg
     2101 tgagcgcgcc atcggccaag atgtcgtcga gacaggtcac ttgctcaaac ctccgtttct
     2161 cagttcaacg ttctgcggct cccgtgcaat ctccttgaac gaaagaagaa tagcgggaat
     2221 gcggaagacc aatgcatgat tgtatgcgaa aatgctggca aaccgggtcc gccacataga
     2281 ttctccatcc ttctcggatg gcttcgtggc gattgcgtcg aagtcgttga cattgcgtcc
     2341 gcgcggccag tagaatgata gtagtagtca atattactac taccccattt ttcgtctgga
     2401 ggcaagagca aattggctgg acttgttgaa accctgcagg acatgggctt gtcccttaat
     2461 gaagccaaag cgtatcaagc gcttctgcgc tatggtgctg ccaatggtta tgaagtgagc
     2521 aagcggtcgg ggatcacgag gtctgtcgtt tatggcgtcc tcgacaggct tctcgagaaa
     2581 ggattcgttc tgcaagtcga ttccgatccg gtgatgtacg ctccgttgcc cccttctcag
     2641 ctgttgaagg aatacaggga actctatgat agaaacctcg acaacctgga acgttctctc
     2701 aagcagatgg agtccggcgt gtcggaggaa agctacatac tgggcgtggc cggatatgag
     2761 gacactatcc gcaaggcccg tgagcttatc cgcgggacga agcatgaggt cgttgtctct
     2821 gcttggggga gcgactgtgt tcctctcaag gaggagctgg aggctgcgga gggcgcggga
     2881 cggaccgtga tcatattctg ccacacgaag gttccattct cggtaggaac catatacgag
     2941 tatgggcttg cagaggacat catcaaggag aaatggccga accggcgcat tattgtggca
     3001 accgattgca ggaatgtgct ggtgggtgat atcaggcatg atgagaacct tggtattgta
     3061 acgacaaatc ctatgattgt gcagatggca gtcgaacacg tgatccttga catcctccat
     3121 ttagcacagc tcaaggccgg gatcggggat cttgccgagc aaattcagag cggtgcagat
     3181 tatctcaggg tcatagaaga tcagcatgcc aagctcgatc ttggtggggc ggtgctgccc
     3241 acgccggttc cattgccatc tgactgagca gacctcgata agtttgggtc tgcattgccc
     3301 gcaattcaga gcctagccgc ccggttaggc caaggtactt ctcgcgaatt atattcagtc
     3361 tgcttgacag acacctggtc ctgtggggga cagggtgaag catcgcggag gtgtagatgg
     3421 gccaaatgaa ctgcactact atggacatcc ccgatgacct tgtggcacgg gccaaaaggg
     3481 atcttgctga tgtgtgggaa ggaagaagca tagagcctgt gatcctactt gccccggagg
     3541 atccagcgct tcccgactac tcggtggccg agaggatgga agatccggaa aaggatctgg
     3601 aataccagtt ggcttccgct cattgggatc tcgcctgcct tcgggcaggc ttttgcaata
     3661 ttccatcgat atggccgtac atgggcgtgg gcgtaatccc ctcagcatac ggttgcgaga
     3721 taaggataac tcccggagaa gacccgtgga ccatccctat cacagatgac cccgacgatg
     3781 tgtatcggct gaaaaagcct gacttggcgc gatcgggact tgccgccaag gtgctcgata
     3841 ggatcggcta tttctccgag atgaccgggg gcactgtgcc gattaggctc acagacgtgc
     3901 aatctcctat ggatactgcg actcagatcc taaattacga ggaactcctg atagcgatgt
     3961 acaccgctcc tgatgcagtt cacttccttc tcgaaatggt gaccgattca ctgatcgagt
     4021 tcgtccggat gcaggaagcg agggcggtca atttcttcgg ccatacccat gtcaaccttt
     4081 ggcggccacg aggcatctat gtgagcgacg acgtgacagc cgtggtatcg ccccagcatt
     4141 accgcgagtt catgaaagcc cctaacgagc gtctctcccg cgctttcgga ggcatatcca
     4201 tccattgctg cagggggtac gagcaaaatc tcatggaagt ggcgaacctc gagggcttca
     4261 tgagctttga cgctgatagc cactacaaca gcatcgattc cataggaagg gctcttgctg
     4321 ctgtggacgg ctgcccaaat tggggcgggg cgggcgagga aaagccgttg cgtgaagacg
     4381 agaggcgatc caggggcatt tggcatgctt gggcagagga tgcagatgaa gccatctaca
     4441 gggtccgagc tgcccggcgc tggggatttg ggatgattct catgctcaag tgcggcggcg
     4501 tggcggaagg cgtggaggct gccagggaga ttcatcgggc cacagaggga ggagtgtggt
     4561 tgactgatga ggattgaccc cctttgtttg gggcagcgtc ggccgagcct gtattctagc
     4621 tcgttgatct aaggatctgt ttgcatgggc gcgtccagtc ttcggaggca gtgggcaaga
     4681 tgcaagatcg agcagagcga ggcatgctgc cccaaacact caaaatgggc ggctatcgcc
     4741 atatcaagaa ctcgtcagat gggccgtaaa gcccttggct tgggttcgta ttcggatacc
     4801 agagaatgtc gaatcatgaa gatgcgggca gggcttgtgg aatccccttt cgcagggaga
     4861 aggtaaacct tcgaggtggc tatggcagca agatcctagc cagcgaccgg ctaagatgac
     4921 atagaggtct cggcaaactg gcagcttcac gtgtgcgatg cgcctatttg caccaaccct
     4981 ttcaagaaca atccccttct gtctcggctg agtcggattg ccatgttaag gccgcaaaga
     5041 gggccttctg gcgggcatag tgcatataca tggaaaacac agcacagact ggcaaggact
     5101 ttgttagcga cctatgcaaa tctccgggcg atacgggaga gatggtccat cgcgttcgca
     5161 gaagtgatta ttgttaatgt gcaggaattc tatgactggc atagaactat actgctaaaa
     5221 gaggattata caagcttcga atggtctcgg aaattaccgg aaacaaggga tgagcaaacg
     5281 gaattgattg tgatctttgt ttagaagcta aattatagtg aagatttcat gagggagcac
     5341 ggacaatgat taagaagaaa atacgcaagg atgacggcag atatctcata tattactcgt
     5401 tccccctaaa cgacgggaaa ggcggggatg agtatctagc tattcgggga gatgatgagg
     5461 gaggggtgac ggacagtgtc ggagatgcgg tggaaccctg tgctcaagca atgggtcata
     5521 actgcaaccc acaggcagga aaggacgtat aagcctcccg aagacttctg tccgttgtgc
     5581 cctacacggg atacatcatt tccaacggaa gtgcctgatt atagctacga gattgtggtt
     5641 tttgaaaaca agttcccttc tttccggcat gttccgcctg aaccggcagt ggaaggcacc
     5701 ggcgtcctca gagtccaacc cgcacagggc atttgcgaag tagtcctcta cacagacgaa
     5761 catcacacca catttgcaga cctcgatatt gaccacatca caaaccttat tgcagtatgg
     5821 acagaccgat atagcgagct cggatcgatc gattacgtag attatgtcct catattcgag
     5881 aacaaggggg acgctgtagg agtgaccctc catcatcccc atgggcagat ctatgcgttc
     5941 ccatatatcc cgccgatccc tgcgaaagag ctcgagtcgg ccgaagagta ccgcgacgct
     6001 accggaaggt gccttttttg cgacattctt gagcaggagc ttgatgacgg gaggcgggtg
     6061 atcgccggca actcgcattt cgcggctgtc gtgcctttct acgcccgata tccatatgag
     6121 gtccacgtta catcgaaaag
//
LOCUS       DAOSAI010000107         6088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8226_length_6088_cov_3.420852, whole genome shotgun sequence.
ACCESSION   DAOSAI010000107 DAOSAI010000000
VERSION     DAOSAI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8226_length_6088_cov_3.420852"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /gene="rny"
                     /locus_tag="PLM83_04930"
     CDS             complement(<1..1147)
                     /gene="rny"
                     /locus_tag="PLM83_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOK70785.1"
                     /translation="MQALIYVAVVALSAYLGYLARKKIAEAKISVAEDAAKNIIEEAE
                     REAEAKKREAVIEAKEEVHRMRSEFDRECRDRRNELQRVERRILQKEESLDRKSDQLE
                     RKESQIDRREKEIDRTQAEIEKIHQEQRQELERIAGLTTDQAREQLLREAEEEITHDV
                     AVMIKEAESQAREEAERRAREIVTQAIQRCAADQVAEGAVSVVALPNDEMKGRIIGRE
                     GRNIRALETLTGIDLIIDDTPEAVILSGFDPVRREVARIALEKLIQDGRIHPARIEEM
                     VERAQKEVDAVIREEGERATFEVGVHSIHPELVKLLGRLKYRTSYGQNVLQHSIEVSH
                     LAGIMASEIGADVAMAKRAGLLHDIGKATDHEMEGPHVTIGADLARKY"
     assembly_gap    1417..1426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1427..2021)
                     /locus_tag="PLM83_04935"
     CDS             complement(<1427..2021)
                     /locus_tag="PLM83_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOK70786.1"
                     /translation="MNGGSGRLDLPGVSGQSGKPGDEHSPVARSSGQSLPWHSDSDVN
                     ASCSRAHSDARAQAYKILSVRARTEKELRDALSKKGHDSAVVDDIVGEMIRCGYIDDV
                     DAAHRWAAMCVDERRHGAKGIAMRLVRRGIDPDLADEAARSAYSAAGLEEFEVALELA
                     EKRVKDDDLCDDKARLRSQRRVAGFLARRGFRSDTIAR"
     gene            complement(2021..3139)
                     /gene="recA"
                     /locus_tag="PLM83_04940"
     CDS             complement(2021..3139)
                     /gene="recA"
                     /locus_tag="PLM83_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOK70787.1"
                     /translation="MRKGRWDLSDREKALEVALTQIEKDFGKGSIMRLGESGGVMQVD
                     AIPTGAIALDIALGVGGMPRGRVAEIYGPESSGKTTVALHVIAEAQKLGGIAAFIDAE
                     HALDATYAKRLGVDIDNLLVSQPDTGEQALEITEALVRSGAVDVIVVDSVAALVPRSE
                     IEGEMGDSFVGLQARLMSQALRKLTGAISKSNTCVIFINQIREKVGIMYGNPEVTPGG
                     RALKFYSSVRLEIRRVESLKQGSEQIGNRTRGKVVKNKVAPPFKEAEFDIIYGEGISK
                     EGSLLDVGEAQGIIIRSGTWYSYGDLRLGQGKENCRQFLKSNPELAAEIEVKVRESVG
                     LPLAGSPASGSGAASDAKDGGKDADGSGKKDGQRKSKS"
     gene            complement(3305..3871)
                     /gene="thpR"
                     /locus_tag="PLM83_04945"
     CDS             complement(3305..3871)
                     /gene="thpR"
                     /locus_tag="PLM83_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOK70788.1"
                     /translation="MDTVRTFIAVPVAGPAAHSVADLIAEMRKVGASVKWVETRNLHL
                     TLKFLGDLPLSRIPALAGAVRKAVEGDAKFEMTLQGAGAFPDVARARTLWVGVDRGAQ
                     RLAGLAEAVDRATVDAGFAPADKAFRPHLTIGRARERSAGAGISRIISENADIYLGAV
                     DVKAIHICSSKLTPSGPIYTSLAEIPLE"
     gene            complement(3996..5264)
                     /locus_tag="PLM83_04950"
     CDS             complement(3996..5264)
                     /locus_tag="PLM83_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOK70789.1"
                     /translation="MKAEIISVGTELLLGQTVDTNAAHIARSLAGWGFSLYRKTTVGD
                     NRARLATVVREALERADVVITSAGLGPTEDDLTKETVAEVLGIHLVESPEVLSDIEGF
                     FASRGTRMTANNRKQALVPEPSSGTFFRNAVGTAPAVVFESGKKRVICLPGVPREMAY
                     LINSGVEPYLRAYLTRNGAGEVIVSRIIRTIGIGEASLEERILDLACEGVNPTVAVYA
                     SEGECQIRVTACAREEKEAWMLIEPVEKELEARLGRSIYGYDDDTLEGVVARRLISAG
                     ARLSVAESCTGGLICGMLTGVPGSSSFLERGIVTYSNSAKRDELGVDESTLRVHGAVS
                     RETAVEMACGVVRTARADLGLSVTGIAGPGGGSADKPVGLVYFCLADTEKTSVITARM
                     MFGGDRRSVRTRACKFGLDLLRRHLEDAWT"
     gene            complement(5343..>6088)
                     /locus_tag="PLM83_04955"
     CDS             complement(5343..>6088)
                     /locus_tag="PLM83_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOK70790.1"
                     /translation="GRRHILEIHTRHKPLAEDVDIDKVAAETYGFSGAHLESLTNEAA
                     IMALRAGRVRITMNDFSEAMEKVIMGERIERKPDEEELRRVAVHELGHAVVSELLKPG
                     SVAAVTVSSRGKALGYVRHAPRSDKYIETAAELEARISVLLAGAMAEELILGERSTGA
                     SQDIQQAVETARKIVNCGLSDLGIISEEDIPRDQYHTAVTRLVQAQEERAKSLLVPNA
                     EFIRKAAGELMSVEKMNGDTFRAQLSQTA"
BASE COUNT         1257 a         1953 c         1441 g         1427 t
ORIGIN      
        1 ggtattttcg cgccagatct gcaccaatag taacatgagg cccttccatc tcgtggtcag
       61 tagccttccc tatgtcatga agcaatcctg cgcgcttcgc catggcgacg tcggctccaa
      121 tctctgaagc cattatccct gcgagatgcg aaacctctat agagtgttgc aggacattct
      181 gcccgtagct cgttcggtat ttgagcctgc ccaaaagctt gaccaattca ggatggatgc
      241 tatgaactcc aacctcaaaa gttgcgcgtt ctccttcctc ccgaataacg gcgtccactt
      301 ccttctgtgc acgctccacc atctcttcaa tgcgggcagg atgaattcgg ccatcctgaa
      361 tcagtttttc aagggcaata cgggcgacct cgcgcctcac cgggtcaaac ccggacaata
      421 tgaccgcctc aggagtatcg tcaatgatga gatcgatccc tgtcaaggtt tcaagtgccc
      481 gaatgttcct tccctcacga cctattatgc gtcccttcat ctcgtcgttg ggaagagcga
      541 ctacggacac ggcgccttct gcaacctgat ccgccgcgca cctctgtatc gcctgtgtaa
      601 cgatctcccg ggcgcgcctt tctgcctcct cccgagcctg cgactcagct tctttgatca
      661 tgacagccac atcatgagtt atctcttcct cagcttctcg taagagctgt tcacgggctt
      721 ggtcagtagt caggccagcg atgcgctcga gctcctgcct ttgctcctga tgaatcttct
      781 ctatttcagc ctgagttctg tctatttcct tttcacgccg atcgatctgg ctttcttttc
      841 tctccagttg atcggacttc ctgtcgagcg actcctcttt ctgaagtata cgcctctcca
      901 cacgttggag ttcgttgcgt ctgtcacggc attcgcgatc gaactcgctc cgcattcgat
      961 gcacttcttc tttggcttcg ataacagcct cgcgcttctt ggcttcggct tccctctcag
     1021 cctcttcaat tatgttcttt gcagcatcct ccgcaaccga gattttggcc tcggcaatct
     1081 tctttcgcgc aaggtatccc agataagcgg agagagccac tacagctaca taaatcaatg
     1141 cctgaataaa tgggctaatg ctaatccacc cccctttacc aaaccaatga ctattgttga
     1201 attgtcggtc taatcacgca ttataactga agccgagcgg taactcggct tcaaacaggc
     1261 ggcggcacga acagcacgcc tgcggcgatg cagttccgga gcaccaaact tgagcatccg
     1321 gctccaacga tgattctata tgtagtttgc agctaatgtc aagctagatg ccatcaacga
     1381 aatagctcgc gcacaacgcg cgcaacgtgc tgaaggnnnn nnnnnncgcg cgcaatggta
     1441 tcggatcgaa atcctcggcg ggcaagaaat ccggctacac gtctctgtga acgcaagcga
     1501 gccttatcat cgcacagatc gtcatctttt acgcgctttt cggccagctc caaagctact
     1561 tcaaactcct caagccctgc agctgaatat gccgatcgtg cagcctcatc agccaaatcg
     1621 gggtctattc cccggcgcac caaccgcata gcgatccctt tcgccccatg gcgcctctcg
     1681 tcgacgcaca tcgcggccca ccggtgggcc gcgtcaacat catcgatgta cccgcaacgg
     1741 atcatctctc caactatgtc gtcgacgact gcggaatcgt ggcccttctt agaaagggca
     1801 tctcgcagct ccttctctgt gcgagcgcgc actgacaaga tcttgtacgc ttgggcccgg
     1861 gcgtcgctat gagcgcgact gcaagacgcg ttcacgtcgg aatccgaatg ccaaggcaaa
     1921 gactggccgg atgatctcgc gaccggggag tgttcgtctc ctggcttgcc agattgcccg
     1981 gagacgcctg ggagatccag ccgcccactg cccccgttca tcaacttttt gatttcctct
     2041 gtccgtcctt cttgccgctg ccatcagcgt ccttgccgcc atctttggcg tcagaagccg
     2101 ccccgctgcc ggacgcgggt gaccccgcaa ggggaagccc aacactctcc ctcaccttga
     2161 cctctatttc tgccgccagt tccgggttag acttgagaaa ctgcctacaa ttctcttttc
     2221 cctgcccaag cctgagatcg ccataggaat accatgttcc acttcggatg atgatcccct
     2281 gggcctcacc tacatcaagc agagacccct ctttcgagat cccttcgcca tagattatgt
     2341 caaactccgc ttccttgaaa ggcggcgcaa ctttgttttt taccaccttt ccacgagtgc
     2401 ggtttccgat ctgttcagaa ccttgcttca gagactcaac ccttctgatc tcaaggcgta
     2461 cggacgagta aaacttgagg gctcgaccac ccggggtaac ttcaggattg ccgtacatta
     2521 tccccacttt ttcacggatc tgatttatga atatcacaca tgtattcgat ttcgatattg
     2581 ccccggtcag tttgcgcaaa gcctgagaca tcaacctcgc ctgcagcccg acgaaggaat
     2641 cgcccatctc accctctatc tccgatcgcg gcaccaatgc cgccactgag tctaccacaa
     2701 taacgtctac ggcaccgctg cgaaccaatg cctcggtgat ctccagtgct tgctctcctg
     2761 tatccggttg ggaaacgagc aggttgtcga tgtccactcc aagccgcttg gcgtatgtag
     2821 catcgagagc atgctctgca tcgataaatg cagcgatccc tccaagcttc tgagcctccg
     2881 cgattacatg gagcgcgaca gtggtcttgc ccgatgattc aggtccatat atctcagcga
     2941 ccctgccgcg aggcatgccg ccaacgccta acgcgatatc aagggctatt gcgccagtcg
     3001 gaatcgcatc cacctgcatc actccaccgg attcgcccag gcgcataatg gaccccttcc
     3061 cgaagtcctt ttcaatctga gtcagcgcca cctcaagcgc cttttccctg tcactcaaat
     3121 cccatcgccc cttccgcatt tcatcccatt tgcacgtcta attcgatttc gtttcatttc
     3181 acctgcgccc ctgtgtaagg agcgacacga tcaagagacg tcctgcattt ctctctgagc
     3241 aatgcgggga cagcccatct agagcatacc acatttcacg cataaccgca catatgttcg
     3301 gtcgtcactc aagagggatt tcggccagag acgtgtagat ggggcccgac ggcgtaagct
     3361 tgcttgaaca gatatggata gccttgacat cgacggcgcc cagatagatg tcggcattct
     3421 ctgagatgat cctcgatata ccagcgcccg cagagcgttc gcgtgctctg cctatggtaa
     3481 ggtgaggcct gaacgccttg tcggcaggag caaatccagc atccactgta gcccggtcaa
     3541 cggcttcggc aagccctgca aggcgctgag cgccacggtc aactcctacc cacagagtcc
     3601 tcgccctggc gacatcgggg aaagcccccg ctccctgcaa cgtcatttcg aacttggcat
     3661 cgccctccac tgccttcctg acggctccag caagcgcagg aatgcgcgac aacggcaagt
     3721 cgcccaaaaa cttcagcgtt aagtggagat tacgtgtttc aacccatttg acagacgcgc
     3781 caaccttgcg catctcggca atgagatccg ccaccgaatg ggctgcaggg cccgctacgg
     3841 gcacagctat gaatgttcgg acagtatcca atgccgtccc ccctttgcag tatcagattc
     3901 cagattagag ccgagaatcc ttcacccgcg tcatcgcccg taagggctat tctccagcaa
     3961 aaagagggtg atttgtggat gcgggaacgt tgcccctatg tccaagcatc ctcaagatgc
     4021 ctcctaagca aatcgaggcc gaacttgcac gctcgcgtcc tcacagaacg ccggtcacca
     4081 ccgaacatca ttcgagcagt aatgacagag gtcttctcag tgtctgccag gcagaagtag
     4141 acgaggccca caggcttgtc ggcgctcccg ccaccggggc ctgctatccc cgttaccgaa
     4201 aggccgaggt ccgcccgtgc cgtccgaacc accccacacg ccatttccac agccgtctca
     4261 cggcttacgg caccatggac gcgcaatgtc gattcgtcca ctccaagctc atctctcttc
     4321 gccgagttgc tgtatgtcac gattcccctc tccagaaagg atgaagatcc gggcacacct
     4381 gtaagcatcc cgcatatgag tcctccagta cacgactcag cgaccgaaag ccttgcgcca
     4441 gcagatatga ggcgcctggc gacaactcct tcgagggtgt cgtcgtcgta cccatatatg
     4501 gacctgccca gcctagcctc gagttccttc tctactggct caatcaacat ccaagcttcc
     4561 ttctcttctc tggcgcatgc cgtgactctg atctgacatt ctccctcgct tgcatatacg
     4621 gctaccgtgg ggttaactcc ctcgcaagcg agatcgagta tacgttcttc cagggaagcc
     4681 tcgccaatgc ctatggttcg gattatcctg gaaacgatga cttcaccagc gccgtttcgc
     4741 gttaaatagg cgcggagata cggctcgacg ccggagttga tgagataggc catctcgcgc
     4801 ggaacccccg gaagacagat gaccctcttc tttcccgatt cgaacaccac tgctggcgct
     4861 gtgcccaccg cattgcgaaa gaacgtgccc gagctaggct cgggcacgag tgcttgcttc
     4921 ctgttattcg ctgtcatgcg agtaccgcgg gaggcaaaga acccttcgat atccgagagg
     4981 acctcgggac tctccacgag atggattccg aggacctcag caacggtctc ctttgtcagg
     5041 tcgtcttcgg tgggtccgag tccggcgctg gtgatcacca catcagcccg ctcaagcgct
     5101 tcgcgaacca cagtggcgag cctcgcccta ttatcgccga cagtcgtttt tctgtatagg
     5161 gagaaccccc acccggcaag cgatctcgcg atatgcgcgg cgttggtgtc caccgtctgg
     5221 cccaagagta attccgttcc gacggatatg atctccgctt tcatgcaaaa ccctccgaca
     5281 ggtccgagca caccgctgtc ggcctgacag agacggccga aagcggattc gtgccacccc
     5341 aatcaggccg tctgcgagag ctgggccctg aaagtatccc cattcatttt ctccactgac
     5401 atgagttcgc ctgcagcctt tctgataaat tccgcattgg ggacgagcaa cgactttgcc
     5461 cgttcctcct gagcctgaac aagcctggtc acggccgtat gatattggtc tcgagggatg
     5521 tcctcctcgc ttatgatccc cagatccgac aggccacagt tgacaatctt ccttgcagtt
     5581 tccaccgctt gttggatgtc ttgtgacgcc ccagtgcttc gctcccccag aatcagctct
     5641 tccgccatcg ccccggcaag aagaacgctg attctggctt cgagctctgc cgctgtttcg
     5701 atatatttat cagacctcgg agcatgtctc acgtatccca gagctttccc gcgcgaggac
     5761 actgttacag ctgccactga ccctggcttg agcagctcgc tcacgacagc atggccaagc
     5821 tcgtggactg cgacgcgccg gagctcttcc tcatccggct ttcgctctat cctttccccc
     5881 atgatcactt tttccatcgc ttcagaaaag tcattcattg tgatccggac ccgcccggcc
     5941 ctgagcgcca tgatcgccgc ttcattggtc aagctttcaa ggtgagcccc ggagaatccg
     6001 taggtctccg cagccacctt gtcaatgtcg acatcctcag cgagcggctt gtgcctggtg
     6061 tgtatctcca gaatatggcg tcttcctt
//
LOCUS       DAOSAI010000108         6028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8332_length_6028_cov_4.229868, whole genome shotgun sequence.
ACCESSION   DAOSAI010000108 DAOSAI010000000
VERSION     DAOSAI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8332_length_6028_cov_4.229868"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3070
                     /locus_tag="PLM83_04960"
     CDS             <1..3070
                     /locus_tag="PLM83_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK70791.1"
                     /translation="IRRCAEESKDGLAEEWDDIVDTLAAGGADVGLLCALDWDSPEFI
                     EEFLKIHSTAQPVRSVVTAKELDKIAKQVREKAANFVYADMMVSTVETVVRACEVLDE
                     TYLWLRGNGASMDFAGLELAARDLLRDTDAGERVRKQIKFVMVDEYQDTNSLQHEIVM
                     ELAGDPPGNRLFIVGDVKQSIYGFRGAEVEVFKGAMDDAQMDGGYAGMEDLGQNFRSI
                     DPLVQLTNYVFSPALCDIWTSDATAFRSMPASAHPRTELHVITYGKSELGRASLAEAD
                     LVASRIAQMVADGEQLVCEHDRETGELVGTRPVRYKDIAVLLKTTSRLENFEAALERY
                     EVPYYVVSGRGFYGRPEVVWVVNMLKAIDDPCDDLSLAAVLRHPVFGLSDESLLRLAL
                     AGGLAHAFCDMDVAVDGWEANERKKLGRARECMESLRRLAGRLSPGGMIQAMLDATQF
                     EPYLAMTADGLKAIGNVHKLAEMAREASRSATSIRDFIDMIERAAIDDAKEAEASLVE
                     EEADVVTVMTVHKSKGLEFPVVIVPELNRGTGSWNTPIFEFAADMGIAVPISPEIRYR
                     DTSFGSLVADELACAENEESMRCLYVASTRASDYLVLTSTLEASANLMSLDGAPEWLM
                     PYGQAVVGSFFAAAGGDVASGCGDAEEVFEAVVALGPGSEALVHVRMLPLAGYMWDKG
                     AEEAGKGPAAGYVSGLALEPAQEAATVPTGSAVLRAESVADQFRLVGPVKVAPDQDDP
                     VQVSVSALMCLDRCPRWYVYEYVLGLSRVQGSLVAPSVGMEMEMRLNAAQRGSVVHSV
                     CQWARNENEAMTLLKKQLGAYGLRGRAFVEAEEELWPLIRNYLDSPEALAEGRREQAF
                     LLDLGAAVVSGIADYIAVDEFMVGTVVDLKTNRLDDEGIDLAARDYRTQMDAYALAVH
                     RGLGAKRVTAVLNFLHPNVRVALEYGQEEFAEAEERLIRLAARTADCRLESTKPEWSR
                     HCRVCYYSRMCWPERDDVDAVQPLDAWHEVEPEVGDDDGFLEAELGGEAE"
     gene            3027..3353
                     /locus_tag="PLM83_04965"
     CDS             3027..3353
                     /locus_tag="PLM83_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70792.1"
                     /translation="MTASSRLNWAERQSKVAGLDESVNMGRAYEIGFEVFGKFCYGVY
                     GVHCWASGVADDRLHLDVPDALIDLRGVPLTITLQNCILRPLSPGSVDSGTWFCFGEI
                     RLRART"
     gene            complement(3759..4475)
                     /locus_tag="PLM83_04970"
     CDS             complement(3759..4475)
                     /locus_tag="PLM83_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70793.1"
                     /translation="MLEIKDLEVYYDGIHAIKGVSLTVPQGTLVTLVGANGAGKSTLL
                     RTICGLKKPSSGRLLYEGHNLLDMGVTQIVKAGITMVPEGRQIFPDLTVIENLHLGAY
                     SRTDQDEIARDRERVFNLFPRLKERAAQKGGTLSGGEQQMLAIARGLMAKPSLLLMDE
                     PSLGLAPILVEEVFRVISEIHSEGVTVLLIEQNAQAALAIADYGYVMESGTIVLQGNG
                     ADLLNDEAVRRAYLGQDVTA"
     gene            complement(4453..5253)
                     /locus_tag="PLM83_04975"
     CDS             complement(4453..5253)
                     /locus_tag="PLM83_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70794.1"
                     /translation="MSKNTVPALVCENVTMKFGGLTAVSDFNLRVPEGALYGLIGPNG
                     AGKTTVFNIVSGVLRPSAGAIKLFGDDITGLSPDRIAYRGIARTFQNIRLFQNLTVLD
                     NVLVGLHLRREVNLAQALLALPQHRAEEARMAQTATQLLEEVGLDDFSDYRANALPYG
                     QQRRLEIARALATKPKVLLLDEPAAGMNPAETENLMRLITDIREEFGVTILLIEHEMK
                     VVMGICERMTVLDHGEIIAEGTPAEIQRNERVIKAYLGGGAVVGNKGS"
     gene            complement(5260..>6028)
                     /locus_tag="PLM83_04980"
     CDS             complement(5260..>6028)
                     /locus_tag="PLM83_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOK70795.1"
                     /translation="NSIQWGFFPALLMAAVVSGIVGFLVGAPAIRFRGDYLAITTLGF
                     AEIIRVVFTNIVPITNGALGLKGIPPHTDLVWSWGFALICIAIMARLMKSSFGRALKA
                     IRDDEIAAEAMGVNLFSHKCLAFVLSAMMAGVGGGLLGNLITTIDPNMFRFPLTFTVL
                     MMAVLGGLGSITGTVISAVLVTVAMEALRALEEPRVIFGLALPGIPGLRMVVFSIALM
                     IVILFFPKGFMGDREISLDRAKRFATRLCRTNTSRQG"
BASE COUNT         1213 a         1491 c         1976 g         1348 t
ORIGIN      
        1 gattagacgg tgtgcagagg aatccaagga tggcctggct gaagagtggg acgacatagt
       61 ggacacgctt gccgcgggcg gtgccgatgt gggcctgctt tgcgcactgg attgggattc
      121 gcccgaattc atcgaggagt tcctgaagat ccattccaca gcgcagccgg tgcggagcgt
      181 agtgaccgcg aaggagctcg ataaaatcgc caagcaggtg cgggagaagg ctgcgaattt
      241 cgtttacgcc gatatgatgg tgtcgactgt ggagactgtt gttagggcgt gcgaggtgtt
      301 ggatgagacg tacttgtggt tgcggggcaa cggagcgtcc atggattttg ccggactgga
      361 gctggctgcg cgggatctgc tgcgtgatac cgatgccggc gaacgggtga ggaagcagat
      421 caagttcgtg atggtagatg agtaccaaga cacgaatagt ctgcagcacg agatagtgat
      481 ggagcttgcg ggggatccgc ctggaaaccg gctgttcata gtgggagatg ttaagcagtc
      541 gatatatggt ttccgcgggg cggaagtgga ggttttcaag ggcgcgatgg acgatgcgca
      601 gatggatggc gggtatgcgg gaatggagga tctgggacag aacttccgct caatcgatcc
      661 tttggtgcag ctcactaatt acgtttttag cccagccttg tgcgatatct ggactagtga
      721 cgccacggcg tttcgaagta tgcccgcctc ggctcatccc cggacggagc ttcacgttat
      781 tacctacggc aaatcagagc tcggacgcgc atcactggca gaggctgatc ttgtggcatc
      841 gcgaatcgcg cagatggtgg ccgatgggga gcagctcgta tgcgagcacg acagggaaac
      901 cggagagctt gtgggaacgc ggccggtgag atacaaggat attgccgtac tgctcaaaac
      961 cactagcagg ctggagaatt tcgaggccgc tttggagcgg tatgaggtgc cgtattacgt
     1021 cgtctccggt agggggttct atggccgtcc ggaggtagtc tgggtagtaa acatgctcaa
     1081 ggctatcgac gacccatgtg atgacctgtc tcttgcggcg gtgctcagac atccggtctt
     1141 cgggctttcc gacgagtctc tcttgcgtct agctcttgca ggcggccttg ctcacgcgtt
     1201 ttgcgatatg gatgtggcgg ttgacgggtg ggaggcaaac gaaaggaaga agctcgggcg
     1261 ggcgagagag tgcatggaaa gcttgaggcg gcttgccggc aggttgagcc ctggcgggat
     1321 gattcaagca atgctcgatg ccacccaatt cgagccatat cttgccatga ccgctgacgg
     1381 actcaaggcc ataggcaatg tccataaatt ggctgaaatg gccagggaag cttcccggtc
     1441 ggctacgtcg atcagggatt tcatagacat gatcgagcgt gcagccatag atgacgccaa
     1501 ggaggctgag gcgtcgctcg tggaagaaga agcagatgtt gtgacggtga tgacggtcca
     1561 caaatccaag ggtttggagt tcccggtcgt aatcgttccc gagcttaacc gaggaactgg
     1621 gagttggaac acgcccatct tcgagtttgc tgcagacatg ggtattgccg tgccgatctc
     1681 gcctgagatc cgataccgtg acacgtcatt tggaagtctg gtcgcggacg aactggcctg
     1741 cgctgagaat gaagagagca tgagatgcct ctatgtggcc tccacccgtg cctccgacta
     1801 tctggtgcta acatctaccc ttgaggcgtc tgccaatctt atgtcgctgg atggagctcc
     1861 tgaatggttg atgccatatg gccaggctgt tgtggggtcc ttcttcgcgg ctgcgggagg
     1921 agatgtggcc tcaggatgtg gagatgctga ggaggtattc gaggctgttg tggctcttgg
     1981 gcctggcagc gaagcgttgg tgcatgtgag gatgcttcct cttgcaggct acatgtggga
     2041 caagggggca gaagaggccg gaaaggggcc tgcggcggga tatgtttcgg ggctggcgtt
     2101 agagcccgcc caggaggcgg caacagtgcc aacaggctca gcagtgctgc gagcagagtc
     2161 ggtggcagat cagttcaggc tggttggccc tgtaaaggtt gctcccgatc aggatgatcc
     2221 cgttcaggtg tcggtttcgg ctttgatgtg ccttgacagg tgtccccgtt ggtacgtcta
     2281 cgagtatgtg cttggccttt caagggttca ggggagcctc gtcgcgccat cggtcgggat
     2341 ggagatggag atgaggctca atgcggctca gcgcgggtcg gtggtccact ctgtatgtca
     2401 gtgggcacgg aatgaaaatg aggctatgac tctcctcaag aagcagctag gggcgtacgg
     2461 gcttaggggt cgggcgtttg tggaagccga agaagagctc tggcccttga tacgcaatta
     2521 ccttgatagc cctgaagcgt tggcagaagg caggagggag caggcgttcc tgctcgacct
     2581 gggcgccgcg gttgtgtcgg ggattgcaga ctacatagct gttgatgagt tcatggtggg
     2641 caccgttgtt gacctcaaga cgaacaggct cgatgacgag ggcatcgatc tggccgcccg
     2701 tgactaccgc acccagatgg atgcgtatgc cctcgcagtt cacagggggt tgggggcgaa
     2761 acgggtcact gcggttctga attttctcca tcccaacgtg cgtgtggcac ttgaatacgg
     2821 ccaggaggaa tttgctgagg cagaggagag actgattcgc cttgccgcga gaacggcgga
     2881 ttgcagattg gagtcgacga agcctgagtg gagccgccac tgtagggtct gttactactc
     2941 taggatgtgc tggcctgaaa gggacgacgt agacgcggtg cagcctttag atgcgtggca
     3001 tgaggtggag cctgaggttg gggatgatga cggcttcctc gaggctgaat tgggcggaga
     3061 ggcagagtaa ggttgcgggg ttagacgagt ccgtcaacat gggcagggca tacgaaatcg
     3121 gctttgaagt ctttggcaag ttctgctatg gcgtttatgg ggtacattgt tgggcttctg
     3181 gcgtggcaga cgacaggctc cacctggatg tgcccgatgc gctgatcgat ctgcgtgggg
     3241 taccattgac catcacatta caaaactgca tattgcggcc tttgtccccg ggttctgtcg
     3301 acagcggaac ctggttttgc tttggagaga ttcgattgcg cgctcgcacc tagacatgtc
     3361 gcctgagaag acggagtagg caatgtcgtt gcacggtaac cggtgaatga caacaccgtt
     3421 gtgtctgcct gttcgaccag tgaaaccagg gcctctggaa ctggcggaca tcaccttgtt
     3481 ggccaggtca tcgaggcgtt tcatcctggc cgcgggtccg agcgggtctg gcgaggagac
     3541 taggttttag acatatcctg cgaaaggtgg ctgagggtgc ctttttgtgg acctactgat
     3601 cgcataatcc gtgccaagcg gagctcaata ggaatacaat gagtggaggc gagcacgaag
     3661 cagcggatct cggagcggaa acatggcctt attctgtctg gaacacggcc agagccagac
     3721 ggaattgggt agattcccct actcgatccg gacctcgcct acgccgttac atcctgccct
     3781 aagtatgcac gccgaaccgc ttcgtcgttg agtaggtcgg ccccgtttcc ctgaagcacg
     3841 atggttcctg attccatcac atagccgtag tcggcgatgg ccagggcggc ctgggcgttc
     3901 tgctctataa ggagcacggt gacgccctcg gagtgtatct ccgaaatcac gcggaagact
     3961 tcttccacga ggatgggggc gaggccgagt gatggttcat ccatgagcag gagcgatggt
     4021 tttgccatga ggccccgggc tattgcaagc atctgctgtt cgccgcctga cagagtcccg
     4081 cccttctggg cagcgcgctc tttcagccgc gggaaaaggt tgaaaacgcg ctcgcgatcg
     4141 cgggcgatct catcttggtc agttcgggaa taggcaccaa ggtggaggtt ttcaatgact
     4201 gtgaggtcgg gaaagatctg tctcccttca gggaccatgg ttatgcctgc tttgactatc
     4261 tgggtcacgc ccatgtcgag caggttatgc ccctcgtaga ggagccggcc ggatgagggc
     4321 ttttttaatc cgcagatcgt tctgagcagg gtgcttttgc ctgcgccgtt agcgcctacc
     4381 aaggtgacga gagtcccttg gggcacggtc agagagacgc ccttgatggc atggattccg
     4441 tcgtagtaga cctcaagatc ctttatttcc aacaactgct ccgcccccca gataagcttt
     4501 gattaccctc tcgtttcgct ggatctccgc cggggtgcct tcggcgatga tctcgccgtg
     4561 atcgaggact gtcatgcgct cgcagattcc cataaccact ttcatctcgt gttctatcaa
     4621 gagtattgtt acgccaaact cttcccggat atcggtgatg aggcgcataa ggttttctgt
     4681 ttccgcgggg ttcattccgg ctgcaggttc atccaagaga aggactttgg gttttgtggc
     4741 cagggcccgg gcgatttcca gtcggcgctg ctggccatat ggaagcgcgt tggcgcggta
     4801 gtcggagaag tcgtccaggc cgacttcctc gagcagttgg gttgcggtct gggccattcg
     4861 ggcttcctca gccctgtgct ggggcagggc gagtagggcc tgtgcgagat tgacctcgcg
     4921 cctgagatgg aggccgacaa gtacgttatc gaggactgtg aggttctgga agagcctgat
     4981 gttctggaaa gtccgggcta tgccgcgata ggcgatccga tccgggctga gccctgtgat
     5041 gtcgtcgccg aagagcttga ttgccccggc agatgggcgg agaacgcctg acacgatatt
     5101 gaagacggtt gtcttgccgg cgccgttggg gccgatcaat ccgtagaggg ctccttccgg
     5161 tacgcgcaga ttgaagtcgc tcaccgctgt gagcccgccg aacttcattg ttacgttttc
     5221 gcagactagt gcgggaaccg tgttcttgga catttcgcat tacccctgtc tgctcgtatt
     5281 tgtacggcac aaccgtgttg caaatctctt tgcccgatcg agcgagatct ctctgtcgcc
     5341 catgaatccc ttgggaaaga agaggatgac tatcatcagg gcgattgaga agaccaccat
     5401 tcgcaacccc ggaattcccg ggagcgcgag gccgaagatg acccggggtt cttcgagcgc
     5461 ccgcaatgcc tccatagcca ctgtgacgag gacggcggag attactgtgc cggtgatgct
     5521 ccccaacccc ccgaggactg ccatcatgag gactgtgaag gtgagcggaa acctgaacat
     5581 gttcgggtcg atggtggtta tgaggtttcc gagtagtcct ccgcctacgc ctgccatcat
     5641 ggccgacaat acgaatgcga ggcatttgtg ggagaagagg ttgaccccca tggcttcagc
     5701 ggcgatctca tcatcccgga tggctttgag cgcccgcccg aagctcgatt tcatgagtct
     5761 tgccatgatt gcgatacaga tcagagcgaa accccagctc cagacgaggt ctgtgtgtgg
     5821 gggaatccct ttaagcccca gggcgccgtt ggtaattggt acgatgttcg taaaaacgac
     5881 gcggatgatc tcggcaaagc ccaaggtggt gatggccagg tagtccccgc ggaagcgtat
     5941 ggccggggcg cccacgagaa agcccacgat tcctgagacg acggctgcca tgagaagggc
     6001 ggggaaaaag ccccactgaa tcgagttg
//
LOCUS       DAOSAI010000109         6002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8376_length_6002_cov_3.760552, whole genome shotgun sequence.
ACCESSION   DAOSAI010000109 DAOSAI010000000
VERSION     DAOSAI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8376_length_6002_cov_3.760552"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..207
                     /locus_tag="PLM83_04985"
     tRNA            132..207
                     /locus_tag="PLM83_04985"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:165..167,aa:Trp,seq:cca)
     gene            complement(276..938)
                     /locus_tag="PLM83_04990"
     CDS             complement(276..938)
                     /locus_tag="PLM83_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOK70796.1"
                     /translation="MAAIEPRELIVRRVAQELKSGQVVNVGIGLPTFVVNHLAHDVNI
                     ILHSENGFVGMGDPPEPGREDPDLVNAGGQPSSIKPGGAFFDSATSFAIVRGGHVDVT
                     ILGGLQVDQDGNLANWAVPGRIIGMGGAMDLVVGAKRVIVAMEHRTKDGRSKVVRKCT
                     LPLTATAEVDLLVTELCVIEFTPEGAVLREIAPDVTIGEVLAATEARLYIPDEVGVMT
                     VV"
     gene            complement(940..1590)
                     /locus_tag="PLM83_04995"
     CDS             complement(940..1590)
                     /locus_tag="PLM83_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOK70797.1"
                     /translation="MPKLRTLDQALEGLTDGMTLMIGGFLAVGSPETLVDEVIRRGVR
                     DLTLIVNDTAMPDRGYGRLFANGQVSTLICCYMGMNPTAIAQKDEGKLAVELCPQGSL
                     AERIRAGGAGLGGILTPTGVGTIAEEGKIKLELEGHTYLLELPLRADIALLRAHVADR
                     AGNLVYRKSARNFNPLMAMAADYVVAEASEIRDIGGIDPDHVMTPGIFVDAVVGVS"
     gene            complement(1609..2424)
                     /locus_tag="PLM83_05000"
     CDS             complement(1609..2424)
                     /locus_tag="PLM83_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOK70798.1"
                     /translation="MDKLIITAALVGAELSREQAPYLPLSPAEIGKAAEEARNAGASI
                     VHLHVRDEEGKPTQDIEVFRKTIEEIKKRTDVIVQISTGGAVGTPIEERMRPLDLAPE
                     MATLTTGTVNFGDEVFMNEPQAIEALAVRMKEKGVKPELECFDVGHIANALRLAKRNI
                     VEEPLYFDFVMGVPGGIPGTVPDLMHCVNSLPPGTMWQVAGIGRAELQLAAVAIVLGG
                     NVRVGFEDNIYYSRGVLAKSNAQLVARIARIAGELGRDVATPDEAREMLELKK"
     gene            complement(2429..2869)
                     /locus_tag="PLM83_05005"
     CDS             complement(2429..2869)
                     /locus_tag="PLM83_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOK70799.1"
                     /translation="MEEVMIRVRMSQSDAHYGGDLVDGAKLMQLFGDVATELLIRHDG
                     DEGLFVAYDMVEFKAPVYAGDYIEARGRIVRTGNTSRAMTFEAHKVIEACRDPKDPSA
                     AFILEEPLLVARASGTCVVPKDKQRKQGNCCGCGQDAETTKKEG"
     gene            complement(2895..3671)
                     /locus_tag="PLM83_05010"
     CDS             complement(2895..3671)
                     /locus_tag="PLM83_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOK70800.1"
                     /translation="MPKVDLERVRPYGDTLDDGKVQLAFSLPLRYGTEAKEAARQLAM
                     KMGFEDVDVVSAHDLGGPFTFFVVYGKCVHSVDVTQLHIPKVEVEQMDYFQINDFIRD
                     KLGRKITVVAACTGSDAHTVGIDAIINMKGYAGEYGLERYPWFRAYNLGAQVPNETLV
                     ARAIETGADAILVSQIVTQKNVHIANLTRLVDLVEAEGIRDDVLLICGGPRISHELAL
                     ELGFDAGFGPGTLPSHVAAYIAQELVRKEHRTSARLTEER"
     gene            complement(3659..5248)
                     /locus_tag="PLM83_05015"
     CDS             complement(3659..5248)
                     /locus_tag="PLM83_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOK70801.1"
                     /translation="MWLLKLNLDTDKIARCRECASDIADEVMKGVERVTTTAIERTVL
                     RLVGVDGVDSAGVPLPNVLVDMLREAGRLGDGAACHMGRAVAATGLSPQAIADKAAAG
                     ELDFASFDDVEDDRARAVLEDAIEASLARIEANAAKRCELIEKLDPGTEPRIYVIVAT
                     GNIYEDVGQSQSAARSGADIIAVIRSTAQSLLDYVPYGATTEGFGGTFATQENFRIMR
                     KALDEVSLETGRYIRLVNYCSGLCMPEIAAMGAMERLDMMLNDSMYGILFRDINMKRT
                     FADQHFSRMLNAYAGITINTGEDNYLTTSDAYEEAHIVLASQFINEALAYRSGLPDEQ
                     IGLGHAFEIDPATTNGFLYEVAQAQMARDIFPNKPLKYMPPTKYMTGDIFKGYLMNGM
                     FNLAGVMTGQSIQLLGMLTEAVHTPHIHDRYLAIRNAKYVFNNARNLGEEITFKPGGI
                     VESRAQQVLNKATTTLEQARETGLMAAIEDGVFAGISRTPDGGRGLDGVVERNPEYWN
                     PFAEEIPARLTRGGRCSSCRR"
     gene            complement(5217..>6002)
                     /locus_tag="PLM83_05020"
     CDS             complement(5217..>6002)
                     /locus_tag="PLM83_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70802.1"
                     /translation="VIGCNHSNDLMLAVRRMREFGAELILVDGAAGRTFLASPDIVGA
                     FVLATGAALNGSANRIAELTERAVHMFQLPRPPADVVQVAEPIMQEGVSAILRADGEI
                     EVFPWKTILGREEEIASAITEDDVALICGRAVGDVLLAEVASRLNQRRRAAPPDTASG
                     PKEPLSPKKQAFMVVAKDPSRLAVGNQGAEAFEASGGQLCVLEKAPVIAISINPTSPW
                     RAPLDPADLLLEISRHIRELPVIDVVAGLSAVGGVAFETQPGY"
BASE COUNT         1168 a         1773 c         1528 g         1533 t
ORIGIN      
        1 gcggcatctg catgccgtat cctgccaggc cgaaacgcat atgcggaatt cctgccacgg
       61 tcatcccgcg ccttgacaat ggctctgatg ggttgtagaa tctatttagt cgcaagatga
      121 agtcttttcg caggggagta gctcaattgg tagagcatcg gtctccaaaa ccgagggctg
      181 cgggttcgag tcctgtctcc cctgccatta tttcaaaagc cacctggcta agctcaggtg
      241 gcttttgctt ttgttcgtgt gcttaggcgt gcgctttaaa caacggtcat gactcctact
      301 tcatccggaa tatacagcct tgcttctgtt gcagccagga cctctcctat cgtcacgtct
      361 ggcgcaattt cgcgcagcac cgccccttca ggggtaaact ctatgacgca gagttccgtg
      421 acgagcaggt ctacttcggc tgtggcggtc aatggcaaag tgcatttgcg caccactttc
      481 gatcggccgt ctttggtcct atgttccatt gctacgatga ctcgctttgc gcctactacg
      541 agatccattg cgccgcccat tccgattatc cgtcccggaa cggcccaatt ggctagattt
      601 ccgtcctgat ccacttgtaa cccgccgagg attgttacat ccacgtgccc tccgcggact
      661 atcgcaaagg acgttgctga atcgaagaat gccccgccag gctttatgga agaaggttgt
      721 ccgccggcgt tcacaagatc cgggtcttca cgacccggtt ccggaggatc tcccatgcct
      781 acaaagccgt tctctgagtg aaggataatg ttcacgtcgt gagcaagatg gttcacgacg
      841 aaggtcggca ggccgattcc aacgttcacg acctgtccgg acttgagttc ttgtgctact
      901 ctccgtacga taagctctcg aggttctatt gccgccactt cagctcaccc ctacaactgc
      961 gtctacgaat atgccagggg tcatcacgtg atctggatcg ataccgccta tatcacggat
     1021 ctcgctggcc tccgctacga cgtagtcagc tgccatagcc atgagcggat tgaagtttcg
     1081 agccgatttg cgatacacca agttgcctgc gcggtctgcc acgtgtgctc gaagaagtgc
     1141 gatgtctgct ctcagtggaa gctctagaag atatgtatga ccttccaact ccagcttgat
     1201 cttcccttct tcggctatgg ttcccacgcc tgtaggcgtc agtatgcccc caagtccggc
     1261 tcctccggcg cgtattctct ctgccagtga gccttgggga cacaattcca ccgcgagttt
     1321 tccctcgtct ttctgagcga ttgcagtggg gttcataccc atgtagcagc agatgagcgt
     1381 agatacttgc ccgttggcga atagtctccc gtatccccga tcaggcatcg ctgtgtcatt
     1441 cacaatcaat gtaagatcgc ggactcccct tcgtatcacc tcgtctacga gcgtctctgg
     1501 cgagcccacc gccagaaagc caccgatcat cagagtcatg ccgtcggtaa ggccttcgag
     1561 cgcttggtca agcgttctca gctttggcac gcttagcact cacctccgtt atttcttgag
     1621 ctccagcatc tccctggctt cgtccggagt ggcaacgtcc ctgcctagtt cacctgcaat
     1681 tcgagcaatt ctggcaacaa gctgggcatt gctttttgcg aggacaccac gggaatagta
     1741 gatgttgtcc tcaaaaccca ctcgcacgtt gccgcctagc actatggcaa cagctgcaag
     1801 ctgaagttca gccctgccta tgccagcaac ctgccacatg gttccaggcg ggagactgtt
     1861 gacgcaatgc atcaggtcgg gcacggttcc aggtatgcct cctggcacgc ccataacgaa
     1921 atcaaaatac aggggttctt ctacaatatt tcgtttggca agtcgaagtg cgttcgctat
     1981 gtgcccaacg tcgaagcact ccagttctgg cttgacccct ttttccttca tcctgaccgc
     2041 cagggcctcg attgcctgtg gttcgttcat gaatacctca tcaccgaagt tgactgtgcc
     2101 tgttgtgagc gtagccatct caggagcgag gtcaagggga cgcattcgtt cctcaatcgg
     2161 agttcctact gcgcctccag tcgagatctg cacgattacg tctgttcgtt ttttgatctc
     2221 ttctattgtc ttgcggaata cctcaatgtc ctgggttggc ttgccttcct cgtctctcac
     2281 gtgcagatgg acgattgagg cccccgcatt cctggcctcc tctgcggctt tgccgatctc
     2341 tgcggggctg agtggaaggt aaggcgcctg ttcccttgaa agttccgccc ctactagggc
     2401 tgcagtgata atgagtttgt ccaattgatt agccctcctt cttagtggtc tccgcgtcct
     2461 gcccacagcc gcagcaattg ccttgcttgc gttgcttatc cttcgggaca acacaggtcc
     2521 cactggcgcg ggcaacaagg agaggctcct ccagaataaa ggctgctgaa ggatcctttg
     2581 gatcacggca tgcttctatg actttgtggg cttcaaaggt cattgcccga gaagtattgc
     2641 ccgttctcac aatgcgtccg cgggcttcga tatagtcgcc cgcatagaca ggggccttaa
     2701 actccaccat gtcatatgcg acgaaaagcc cctcatctcc gtcgtgcctt ataaggagtt
     2761 cagtggcgac gtcgccgaac agctgcatta gcttggcgcc gtccaccaga tctccgccgt
     2821 aatgggcgtc agactgactc atcctgaccc tgatcatgac ttcttccatg gtattctcct
     2881 ttcgtccact cccctcacct ttcttccgtc aggcgcgcgg aagtgcggtg ctcctttctt
     2941 accaattcct gagctatgta cgcggcaacg tgagacggca atgtgccagg cccgaatccc
     3001 gcgtcgaatc cgagctctag ggcgagttca tggctgattc gcggaccgcc gcaaatcagg
     3061 agcacatcat cccttatacc ttcagcttcg accaagtcta ccaggcgcgt gaggtttgct
     3121 atatgcacgt tcttctgcgt cacaatctga gaaactagga tggcgtcagc gcctgtttct
     3181 atagccctgg caaccaaggt ctcattggga acctgggccc ccaggttgta tgctcggaac
     3241 caggggtacc gttccagtcc gtattcgcct gcatagccct tcatattgat tatggcgtcg
     3301 attccgacgg tgtgggcgtc agaacctgta cacgcggcga ctactgtgat cttgcgacca
     3361 agcttgtctc tgatgaaatc gtttatctgg aaataatcca tctgttcgac ttctactttg
     3421 ggtatgtgaa gctgtgtgac gtccaccgag tgcacgcatt tgccatatac gacaaagaag
     3481 gtgaatggac ctccaaggtc gtgggcgctc acgacgtcca catcctcaaa ccccatcttc
     3541 atcgccagct ggcgcgctgc ttcttttgcc tcagtcccgt acctcaaggg gaggctgaat
     3601 gcaagttgca cttttccgtc atcaagggtg tcgccgtatg gtctgaccct ttccaaatct
     3661 accttcggca cgaactacac ctcccacccc ttgtgagccg ggccggtatc tcttccgcaa
     3721 atgggttcca gtattctgga tttctttcca cgactccgtc gagtcctctg cctccatcgg
     3781 gcgtccggga gataccggca aataccccat cttctatagc cgccatcaat cctgtctccc
     3841 gcgcttgctc taatgttgtt gtagctttgt tgagaacctg ctgagctcgg ctttccacga
     3901 ttccgccggg cttgaaggtt atctcttcgc ccagattgcg tgcgttgttg aagacgtatt
     3961 ttgcgttgcg gattgcaagg tatctgtcgt gtatgtgcgg agtgtgaacg gcttcggtga
     4021 gcatgccgag aagctgtatg gactggcctg tcatcactcc tgccaagttg aacataccgt
     4081 tcatgagata ccctttgaat atgtcgcctg tcatgtattt tgtgggcggc atgtatttaa
     4141 gcggtttgtt ggggaatatg tcccgggcca tctgcgcttg cgccacttcg tagagaaacc
     4201 cgttagttgt ggccgggtcg atctcgaatg catgccccag gccgatctgc tcgtcaggca
     4261 agcctgatcg gtaagcgagc gcctcattaa tgaactgcga cgccagcacg atgtgggcct
     4321 cttcataagc gtctgaagta gtaagatagt tgtcctctcc agtgtttatg gttatgccgg
     4381 cgtaggcatt gagcatacgc gaaaagtgct ggtctgcaaa ggtgcgcttc atgttgatgt
     4441 cgcggaagag tatgccgtac attgagtcgt tgagcatcat gtcaaggcgc tccattgctc
     4501 ccatggccgc tatctcgggc atgcacagtc ctgaacagta gttgacgagg cggatgtacc
     4561 tgcctgtctc gaggcttacc tcgtcaagag cctttcgcat gatgcggaag ttctcctggg
     4621 tagcaaatgt tccgccaaac ccctctgttg tggcgccata tggcacgtaa tcgagcaagc
     4681 tctgggcggt gcttcgtatc accgcgatta tgtctgcgcc tgatctggcg gcagattgag
     4741 attggccaac atcctcatag atgttccctg tcgccactat gacgtatatg cgcggctcag
     4801 ttccagggtc aagcttctca atgagctcgc aacgctttgc ggcgttcgcc tctattctgg
     4861 caaggcttgc ttcaattgcg tcttccagga ctgctctagc ccgatcgtcc tcgacgtcat
     4921 cgaaactggc gaagtcgagc tcgccggcgg cagctttgtc ggcgatggcc tgcggagaaa
     4981 ggcctgttgc cgccacggcc ctccccatat ggcaagcggc tccgtctcct agtctccctg
     5041 cttcgcgcaa catatcgact aagacattgg gaagcgggac ccctgcggaa tcgacgccgt
     5101 caacgccgac taggcgcaag actgtacgct ctatggcagt cgtagttaca cgctctacac
     5161 ctttcattac ctcgtctgct atgtcgcttg cacattccct gcatcgcgct atcttatcag
     5221 tatccaggtt gagtttcaaa agccacacct cccactgccg agagccccgc aaccacgtca
     5281 attacaggga gctctctgat atgtcttgag atctcgagta gcagatctgc tggatctaaa
     5341 ggcgctctcc acggggacgt ggggtttatg ctgatcgcga tcactggcgc cttctcgaga
     5401 acacacagct gtcccccgga tgcctcgaat gcctctgctc cttggtttcc gactgccaac
     5461 cgcgagggat ccttggcaac gaccataaat gcctgctttt ttggggacaa cggttcctta
     5521 ggcccgctgg ccgtgtctgg cggcgcggct ctgcgtctct gattgagcct cgaggcgacc
     5581 tcagccagaa gaacatcgcc caccgccctc ccgcatatga gggcaacatc gtcttctgtg
     5641 atggccgagg caatctcctc ttcccttccc aggattgtct tccatggaaa gacctcaatc
     5701 tctccatctg cccgaagtat tgccgagact ccttcttgca ttatgggctc tgcaacttgc
     5761 accacgtctg ccggaggccg gggcaactgg aacatgtgaa cagcgcgctc cgtcaattcg
     5821 gcaatcctat ttgccgaacc gtttagagcc gcccctgtgg ccaggacgaa ggccccgact
     5881 atgtcgggcg atgcgaggaa tgttcttccc gccgctccgt ccaccaagat gagctctgcg
     5941 ccgaattctc gcattcttct aacagctagc atcaggtcgt tcgaatggtt gcaccctatc
     6001 ac
//
LOCUS       DAOSAI010000110         5998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8383_length_5998_cov_4.546189, whole genome shotgun sequence.
ACCESSION   DAOSAI010000110 DAOSAI010000000
VERSION     DAOSAI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8383_length_5998_cov_4.546189"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PLM83_05025"
     CDS             complement(<1..888)
                     /locus_tag="PLM83_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOK70803.1"
                     /translation="MRQEQSGRNSVQVGIHNRQLVLDVIRTNQPITRREIARLTGLTS
                     AAVTNIVSNFIEQGFVREVGYGRSGGGRKPKLVELDKGSRIMVAVDLAGADQIAALLD
                     MDGNVLCRGERRIGSAKGVTVADIVDAINEILDTPEARSGQVVGIGVTTPGLVDAATG
                     TVLKSVALNWFNVPIKAALQKHFEYPVFIGKDTHVALLAEEWYGAAKNMQNAIYLWVG
                     PGIAIGMLLDGRVYTGSTGMAGEFGHVSIDYDGPACKCGSWGCMEQMASLRAISSMAA
                     KSDRDAEGYSGDPEERYIDP"
     gene            1407..1949
                     /locus_tag="PLM83_05030"
     CDS             1407..1949
                     /locus_tag="PLM83_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOK70804.1"
                     /translation="MKIGPRIKNLRLKKGLTQEELGERTDLTKGYISQIERDLSSPSL
                     ETFFDILEVLGCTPKEFFSDEEREQKVVYTDEDLTEFVDDERGYRIQWLVPESNENEM
                     EPIMLTLEKGGSFKQFEPSLADTFAYVLSGRIKVSLGKRTYYAKAGQTVYYQASEEHQ
                     ISNDNDGPSELLLVATDSYL"
     gene            2009..3115
                     /locus_tag="PLM83_05035"
     CDS             2009..3115
                     /locus_tag="PLM83_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70805.1"
                     /translation="MAKNTIIRFKNVTKQYDDDPPVLTDVSFELERGKFYTLLGPSGC
                     GKTTIIRLIAGFVEPTVGEIYFDDQEISKIPPDKRRVNTIFQDYALFPHLNVFENVAF
                     GLRVKRMKEDVIREKVREALRFVNLEGLENRDIAGMSGGQQQRVAIARAIVNEPEVLL
                     LDEPLSALDLKLRTEMQYELRELQRSLGITFVFVTHDQEEALALSDEVFVLNNGRIEQ
                     RGTPMDIYDEPVNRFVADFIGESNIISATMIKDYLVEFVGNQFECVDKGFHPNEPAEV
                     VIRPEDLEITPKDQGKLQVYVDSQLFRGVHYEICCYDKEGNEWLVHSTKKVAVGDEIG
                     LNFGPEDIHVMRLGETEDEFDRRLESYEEVRHAG"
     gene            3105..3905
                     /locus_tag="PLM83_05040"
     CDS             3105..3905
                     /locus_tag="PLM83_05040"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017691799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70806.1"
                     /translation="MQAKARNIYLIPYMLWIIMFVIAPIVLIVYYSMYDIDGVWTLAN
                     YKRFFTPVYLRMTLRSFWYAILITGVSLIVAYPTAYLLTKTAHKELWLLLIILPSWIN
                     LLLKVYAFIGILGLYGPINSFLELVGIGAKQILFTDFSFIFVAVYIFIPYMILPIFNA
                     LEELNPSLVAAARDLGASPWATFRRVVLPLTMGGVKSGCRAVFIPALSLFMITRLIAG
                     NRVITLGTAIEQHFLVTQDWGMGSTLAVFLIIVMGLTMLVTESGSRVK"
     gene            3907..4710
                     /locus_tag="PLM83_05045"
     CDS             3907..4710
                     /locus_tag="PLM83_05045"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70807.1"
                     /translation="MKKTGKLSVIYLVLVFIILYTPIFYLIYYSFNSGGSMHHFQEFT
                     LDWYEEVFHDTRLFIILLNTLVVALLSSAISTIIGVAGALAITRVRSKRTRNALLTLN
                     NVMIVSPDVIIGASFLILLTAIGVNLGFFSVLISHIAFSVPIVVLMVLPKLEEMSPTL
                     VDAARDLGASSRDVLTKVVIPYISPGIFAGFFMALTYSLDDFAVTFFVTGGGFSTLSV
                     EIYSLARRGISLTINALSTLIFLATLALVIGYYIITKRGSKPSGAEARE"
     gene            4707..5780
                     /locus_tag="PLM83_05050"
     CDS             4707..5780
                     /locus_tag="PLM83_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012576295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK70808.1"
                     /translation="MKKVTQMFTIVFAAAFALMYLTSYLNALHGYSGPNTLTIYNWGD
                     YIDPYLIKEFERETGLKVIYQTFDSNEAMLTKIAHGGVTFDVVIPSDYAISKMKEQEL
                     LIPLDHSKLPNLKHIDARFLDLPFDEKNVYSIPYFWGTYGIIYNPEVITDIDFDSWDD
                     LWDPRLQNQILLVDSAREVIGMGLNSLCYSLNDTDMDHLQQAKAKLDMLIPNIRAIVG
                     DEIKMLLETEEAAVAVVWSGDASEIIGENDKFDYVIPKEGSNVWFDNMVIPTTAKNVE
                     GAHQFINFMLDPENAAQNAEYVGYSTPNAEALRYLPEEITSDERFYPGSEITDKLEVY
                     ENLSKRMTAYYNELFLEFKMHRK"
BASE COUNT         1454 a         1563 c         1506 g         1475 t
ORIGIN      
        1 tggatcgatg taccgctctt cagggtcgcc gctgtacccc tccgcgtctc tgtcggattt
       61 tgcagccata gaagatatcg cccttaggct ggccatctgt tccatacaac cccagcttcc
      121 acacttgcac gctggcccgt catagtcgat gcttacatgc ccaaactcgc cagccattcc
      181 agtcgaacct gtgtaaaccc gtccatccag cagcatgccg atggcaatcc ccggccccac
      241 ccaaagataa attgcgttct gcatgttttt ggccgcaccg taccattcct cggcaaggag
      301 ggcaacatgg gtgtctttac cgatgaacac cggatattcg aaatgcttct gaagggctgc
      361 cttgataggc acattgaacc agttcaaagc gactgatttg agcaccgtgc cagtggcagc
      421 atcaaccaga ccaggagtgg taacaccgat tccaaccacc tggcctgagc gcgcttccgg
      481 ggtatccagt atctcattta tggcgtcaac tatgtcggca acagtgactc ctttcgcaga
      541 cccgatgcgc cgctctccac ggcaaagcac gttgccgtcc atgtccaaga gcgccgcgat
      601 ctgatctgcc ccggcgaggt ccactgcgac catgattctt gatcccttat caagctccac
      661 cagctttggt ttgcgtcctc ccccggatct cccataacca acctcacgca cgaaaccctg
      721 ctcgataaag ttgctgacga tgttggtgac cgcggcagaa gtaagccctg tgagtcttgc
      781 tatctctcgc ctcgtgatgg gctggttcgt ccgtatgacg tccagcacaa gctgtctgtt
      841 gtggataccc acctgaaccg agtttctgcc cgactgttct tgcctcacat tcccacaatc
      901 cttttactaa gactttggct ttcagtttga taaagtagtt ctccatctgc ttggaaactc
      961 ctcctccttt ctccagaaag ttttgtgccg cgcccatttg tgtgtttggc tcgaggtaaa
     1021 cagatgcaat tcactcggca gtcttgtatg tcggggcaga catactcatg atgacgagaa
     1081 catttcgcag gagcgtaccc gcgtatgagt ccagttgggt atttaataag ccatctagaa
     1141 ggcttccaca gccagccgag cctcggacca cagacgtcgt acatcaacct ttaagtgtta
     1201 tctaaggatt gtcatatgtg ctatgcccaa aatcagtagt gacattatca cagacgcgta
     1261 acacgcgtcc gaaattggca ttgcactttt gcaggaatcg tatataatat cttagcgtcg
     1321 gcagtaaact tggagtttac ctgtgttgaa cttttcaact tgaagtttac ccggcctaaa
     1381 cccaacagat aagcgaggag cgacggatga agatagggcc aagaatcaag aaccttcggc
     1441 tgaaaaaagg tctaacccag gaagagctgg gcgaacgcac tgatttaacc aaaggctaca
     1501 tatcccaaat cgaacgcgac ctgagctcgc cgtcgctgga aaccttcttc gacattcttg
     1561 aagtgctcgg ctgcacgccc aaggaattct tcagcgacga agaacgtgag caaaaggtcg
     1621 tgtacacaga tgaggacctg accgaattcg tcgacgatga acggggttac cgcatccagt
     1681 ggctggtccc tgaatcaaat gaaaacgaga tggagccgat tatgctcacc ctcgaaaaag
     1741 gcggaagctt caaacagttc gagccttccc ttgccgacac ttttgcctac gtgctgagcg
     1801 gaaggatcaa agtttcgctc ggaaaacgaa cttactatgc taaggcaggc caaaccgtct
     1861 actaccaagc atctgaagag caccagattt ccaacgacaa tgacggacca tcagaattgc
     1921 ttctggtggc aactgattcc tatttatgat gtaaagagtt gtgcatcgtg catgatatcc
     1981 tacttccatc cgcggggagg ggacaagaat ggccaaaaac acaatcatcc gtttcaagaa
     2041 cgtcaccaaa caatacgacg atgacccccc ggtgttgaca gatgtcagct ttgaactgga
     2101 gcggggcaaa ttctacacat tgctcggccc ttccggctgc ggaaaaacga cgatcatccg
     2161 cttaatcgca ggcttcgttg agcccacagt cggcgagatc tactttgacg accaggagat
     2221 aagcaagatt ccgcctgaca agcgccgggt aaacaccata ttccaggact atgcattgtt
     2281 tcctcatttg aacgtgttcg agaacgtcgc cttcggactg cgcgtcaaga ggatgaaaga
     2341 agacgtgatt agggagaagg tccgggaagc gctacgtttc gtcaatctgg aaggcttgga
     2401 gaaccgggac attgcgggta tgtccggcgg ccagcagcaa cgcgtggcca tcgcgcgagc
     2461 catagtcaac gaaccggaag tgctgcttct ggacgaaccg ctttcagccc ttgatctgaa
     2521 gctccgcacg gaaatgcagt acgagctgag agagcttcag cgcagtctgg gcattacatt
     2581 cgtctttgtc acacatgacc aggaagaagc ccttgcactt tccgatgagg tttttgtctt
     2641 gaacaacggt cggattgagc agcgcggaac gcccatggac atctatgatg agccggtcaa
     2701 caggtttgtg gctgatttca taggcgaatc caacattatt tcagccacga tgatcaaaga
     2761 ctaccttgtc gaatttgtcg gcaatcagtt tgaatgcgtg gacaagggat ttcatcctaa
     2821 cgaaccggcc gaagtcgtga ttcgtcccga ggacctggag atcacgccga aggatcaagg
     2881 gaagcttcag gtttacgtgg attcccagct cttccgcggc gtgcactacg agatttgctg
     2941 ctacgacaag gaaggcaatg aatggcttgt ccattccacc aaaaaagtag cggtaggcga
     3001 tgagatcggg ctcaattttg gccccgaaga catacacgtg atgaggttgg gcgagacgga
     3061 agacgaattc gacagacgtt tggagtcgta tgaagaggtg cgccatgcag gctaaagcgc
     3121 gcaacatcta ccttatcccc tatatgctat ggatcatcat gttcgtcatt gcgccgatcg
     3181 tgctaattgt ctactactcc atgtacgaca ttgacggagt atggactctc gcaaactaca
     3241 agagattctt taccccggtg tacttgcgaa tgacgctgag gtcgttttgg tacgccatct
     3301 tgatcacggg agtctcgctg attgttgcat atccgacggc atacttgctg accaagacag
     3361 cacacaagga gttgtggctt cttctcatca tattaccgtc atggataaac ttgcttctca
     3421 aggtgtatgc cttcataggc atcttggggc tctacggtcc catcaactcc tttctcgagc
     3481 ttgtgggcat tggggcaaaa cagattctgt tcacagattt cagcttcatc tttgtagctg
     3541 tttacatctt catcccatac atgatactgc ccatattcaa cgcccttgag gaactcaacc
     3601 cgtccttggt tgcagcagcc cgcgacctcg gagcatctcc ttgggcaaca ttccgtcggg
     3661 tagtactccc cttgacgatg ggcggggtga agtcgggctg tcgagcggtt ttcatcccgg
     3721 ccttgtcgtt gttcatgata acgcgcctga ttgccggaaa cagggtaatc actctgggga
     3781 ccgccattga acaacatttc cttgttacgc aggactgggg catgggttcg acccttgccg
     3841 tctttctgat cattgtaatg ggactgacga tgcttgttac agaaagtgga agtcgggtga
     3901 agtagcatga agaaaacagg aaagctgtcc gtcatctatc tggttttggt attcatcata
     3961 ttgtacaccc ctatcttcta cctgatttac tactcattta acagtggagg ttccatgcac
     4021 cattttcagg agttcacgct tgactggtat gaagaggtgt tccacgatac acgcctcttc
     4081 atcatccttc tgaatacact ggtagtcgcg cttctctctt cagcgatatc gacgattata
     4141 ggggtagcgg gggcacttgc catcacgcgg gtgaggagta aacggacccg caatgcgctg
     4201 ctcactttga acaatgtgat gatagtcagc ccggatgtga tcatcggagc gtctttcctg
     4261 attctcctga cagcgatcgg tgtcaatcta ggattcttct ccgttttgat ctctcatatt
     4321 gccttcagcg tgcccatcgt cgtcctgatg gtgctgccaa agctagagga gatgagtccg
     4381 acattggtgg acgccgcccg ggatctagga gcgagcagcc gggatgtgct aacgaaagtg
     4441 gtgatcccct atatttcgcc tggcatattc gcaggttttt tcatggcctt aacctactcc
     4501 ctcgacgatt tcgcggtcac ctttttcgtg acgggaggcg gcttttcaac gctgtcggtg
     4561 gagatatact cgctggcaag gcggggaatc tcgttgacca ttaatgcgct ctctacgctg
     4621 attttcctgg ctaccttagc attggtcatc ggttactaca tcataaccaa gcggggatcc
     4681 aagcccagcg gagcggaggc acgggaatga aaaaggttac tcagatgttc accatagtct
     4741 ttgccgcagc attcgcgctg atgtacctaa cttcatacct taatgccttg cacggctatt
     4801 cgggcccaaa tacgctcacc atttataatt ggggagatta tattgaccct tatttgatca
     4861 aggaatttga acgtgagaca ggcctcaagg tcatctatca gaccttcgat tccaatgagg
     4921 cgatgttgac aaagattgcc cacggcggtg tcacgttcga tgtagtgatt ccgtctgact
     4981 atgccataag caagatgaag gaacaggaat tgctcattcc cctggatcat tctaagcttc
     5041 caaacttaaa gcacatcgat gctcgtttcc tggatttgcc cttcgacgag aagaacgtgt
     5101 attccatccc ctatttctgg ggaacgtacg ggatcatcta taaccccgag gtaatcacgg
     5161 acatagactt tgacagctgg gatgatctgt gggatccacg cctccagaat cagatcttgc
     5221 ttgtagacag tgcccgcgaa gtgatcggga tgggcctgaa cagtctctgc tattcattga
     5281 acgatactga tatggaccat ctgcagcagg cgaaggcaaa acttgacatg ctcataccca
     5341 atattcgagc aattgttggg gatgagatta agatgcttct cgaaactgaa gaggccgcag
     5401 ttgctgttgt ctggtcggga gacgcctcgg agatcatcgg cgagaacgac aagttcgact
     5461 acgtcatccc aaaggaaggg tcaaacgtat ggtttgacaa catggtgatt ccgaccacgg
     5521 cgaagaacgt ggagggagcg caccaattca tcaacttcat gctagatcct gaaaatgctg
     5581 cccagaacgc ggaatacgtc ggctactcga caccaaacgc cgaggccttg aggtatctcc
     5641 cagaagagat cacctccgat gagcgtttct acccgggctc tgaaataacg gataagcttg
     5701 aggtgtacga gaatctcagt aagaggatga ctgcctacta taacgagctc ttccttgagt
     5761 tcaagatgca tcggaagtag caaatcacgt ctcgtatcgg gcccgcaacg cgcgttgcgg
     5821 gccgatgtaa tgcttctttc tgcctaaaac tccatcccca gcgacagcct gtggctgtca
     5881 ggtagggtat tgtggtgctg gtaggcgtaa tcgatgttga aggtcgctgc ttcaaagcct
     5941 gcccctgcag tgaggcatgt tccattcccg ccaaaagagc cgcctgcgcg gagcgcca
//
LOCUS       DAOSAI010000111         5967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8442_length_5967_cov_3.762686, whole genome shotgun sequence.
ACCESSION   DAOSAI010000111 DAOSAI010000000
VERSION     DAOSAI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8442_length_5967_cov_3.762686"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLM83_05055"
     CDS             complement(<1..602)
                     /locus_tag="PLM83_05055"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOK70809.1"
                     /translation="MSSYGADIGIDFGSDNVRVYMPGKGVVIDEASVVVFHRETGAMI
                     EVGDRGRELLLADPGSCVPVRPLVFGSIRRHDAALLLMKRMIWRVLGGRGFTKPRVVL
                     TVPSCPSDVEIRAWMTMATEAGARSVMLLEAPMAAAMGADMNVFDARGQMMVDVGAEV
                     TDIGVIAVGEMIVSERVRTGGRAMDAAIQKHIRADKGLVLG"
     gene            complement(708..962)
                     /gene="spoIIID"
                     /locus_tag="PLM83_05060"
     CDS             complement(708..962)
                     /gene="spoIIID"
                     /locus_tag="PLM83_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HOK70810.1"
                     /translation="MREYIRRRVLEITDHILETQATVRQTARLFNVSKSTVHKDVTER
                     LPSIDKQLAKKVKKILDQNKAERHLRGGEATRRKYKSGNK"
     gene            complement(1029..1718)
                     /locus_tag="PLM83_05065"
     CDS             complement(1029..1718)
                     /locus_tag="PLM83_05065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK70811.1"
                     /translation="MKWPFGSRSTSMMLVVGIAAFFLGFSLAILVSNVRRDTPAQIVG
                     HTDSGRFESEADEAIAIKPHEESGADSIEGVNAISAADSGSLSESEQGLVSMPEAMTA
                     PVDGSVVAGYGWRRDPVMDDWRFTDGIDISCLPGTQVRAALSGKVAGVDRDERGVLVV
                     RLEHAGGVETVYENLRIAWVKAGDEVGQGASLGEVGASSAASDGECIIRFIVLERGNA
                     IDPVQCIGKRF"
     gene            complement(1809..2792)
                     /gene="spoIID"
                     /locus_tag="PLM83_05070"
     CDS             complement(1809..2792)
                     /gene="spoIID"
                     /locus_tag="PLM83_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HOK70812.1"
                     /translation="MSVSRFAWSLVSICVIVIIVLPGAIALFVRPRTAPGTTASLWVD
                     LWDCRAGTVRRMPLADYLVGVVAAEMPAAFDLEALKAQAVAARTYTLRKMWQTQETSA
                     STDVHKGAVICSDPSHCQAWSSREELLKKWGVAGYLANIRKVIAAVEETEGLVMTYNG
                     RLIDAVYHSCCGGMTEDAAAVWGRRVPYLVPVSCGCQQKALELGEMKTLEGAELVKAL
                     GLGSRSLPAMSSGVQVVARTATARASMVSVYGVPVSAADLRRALSLKSTRLAVVSSGG
                     KVTITTTGYGHGVGMCQWGAENLARAGLTFDAILKHYYSGIEIQRLDSSRK"
     gene            complement(3270..4547)
                     /gene="murA"
                     /locus_tag="PLM83_05075"
     CDS             complement(3270..4547)
                     /gene="murA"
                     /locus_tag="PLM83_05075"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOK70813.1"
                     /translation="MDSFVIKGGESLHGRLRVGGAKNASLPILAASLLACGSSTVEDV
                     PELLDVETMCGVLTSVGARVSRSKNKVVVDATEISSFEPPYDLVRAMRASFLVMGPLL
                     ARMGCARIALPGGCAIGSRPIDLHLKGFAALGATIDMGYGQIEARADRLEGARVYLDF
                     PSVGATENIMMAAVLASGTTIIENAAQEPEIVDLANFLNSMGARVRGAGTTVLRIEGV
                     PELSPTSYMVVPDRIEAGTFMIAPAIAGGSLVIENVVSEHLKPITAKLRECGVTVEEN
                     DDGSMFVEMNDRPRGVDIKTMPYPGFPTDLQAQFMALMTICSGSSVITETVFENRFMH
                     VAELMRMGANIKIDGRSAVVEGQDKLLGAQVKCTDLRAGAALVLAGLAAEGDTEVADI
                     YHIDRGYENFEGKLASVGANIVRVASAEAEQAV"
     gene            complement(4646..5803)
                     /gene="wecB"
                     /locus_tag="PLM83_05080"
     CDS             complement(4646..5803)
                     /gene="wecB"
                     /locus_tag="PLM83_05080"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOK70814.1"
                     /translation="MGRIKVLSVFGTRPDTIKLAPVVAELSKYPDMISSVTVGTAQHR
                     DMMDQVLSVFGISPDYDLGIMQDDQTLFDIAERGLRGLEEVLVREKPDITLVHGDTST
                     AFVGALASFYCKTAIGHVEAGLRTYQKYDPFPEEMNRRLVGCLADLHFAPTADHLENL
                     LRENFPRENIYVTGNTVVDAVMRVAQSRTGYDDPKLASLDLEGKRLILVTAHRRENIG
                     EPLRNICAAIRDVLADYPDVVVVMPVHLNPRVRETVMEELSGAERCFLVDPVSYPDMV
                     GLMEACYMVMTDSGGLQEEAPALGKPVLVLRRTTERPEGLRTGTLRLVGVGREQIREE
                     AGILLEDAEQYRRMASAPNPYGDGNAAHRTVMGILHHYGLVDERPSDFMPI"
BASE COUNT         1431 a         1879 c         1325 g         1332 t
ORIGIN      
        1 cccaacacta gccccttgtc ggcgcggata tgcttctgaa tcgctgcatc cattgcgcgc
       61 ccacccgtcc tcacgcgttc ggagactatc atttctccga cggcaatcac ccctatgtcg
      121 gttacttctg cacccacgtc taccatcatc tgcccgcgag catcgaatac gttcatatcc
      181 gcgcccatcg ccgccgccat tggcgcttcc agcaacatca cacttctcgc cccagcttca
      241 gtcgccatgg tcatccaagc tctgatctct acatctgatg gacatgacgg aaccgtcaac
      301 accaccctgg gtttcgtaaa accgcgtcct cccagcaccc gccatatcat ccgcttcatt
      361 agcaacaacg ccgcgtcgtg tcgacggatc gatccaaaaa ccaaaggcct aacgggcaca
      421 caggaccctg ggtccgcaag cagcagctct cgaccgcggt cgccgacctc aatcatagcg
      481 cctgtctccc tgtgaaagac aacgaccgag gcctcgtcta taacaactcc ttttccaggc
      541 atgtacaccc tcacgttatc agaaccgaag tctattccga tgtccgcccc gtacgacgac
      601 atgttgcacc cccaaaagca gattaaggga aggaccggcg cgggcccgcc tatgccggtc
      661 ttctcgatgg tcgcatctca gcctttctca gattcccacc caccctgtca tttgttgccc
      721 gacttgtatt ttctccgggt cgcctcacca cccctgagat gtctttccgc cttgttctga
      781 tccagaatct tctttacttt tttcgcaagc tgcttgtcta tggaggggag cctctccgta
      841 acatccttgt ggacggtgct cttgctcaca ttaaacaggc gtgccgtctg tcgcactgtt
      901 gcctgggtct ccaggatgtg gtcggttatc tcgagaacac gccggcggat gtattccctc
      961 atctagcgtc cggcctcccc cctgccgttt gctagatcta tattcgctca ggggtggatt
     1021 atgcctagct agaagcgctt gccgatgcac tggacagggt cgatcgcgtt tccccgttcc
     1081 aaaacgatga atctgataat gcattcgcca tcagaagccg cggaactcgc gccaacctct
     1141 cccaaactag cgccctggcc aacttcgtct cccgccttga cccaagcaat cctcaggttc
     1201 tcgtagacag tttcaacacc ccctgcgtgt tccaatctta caaccaagac tccacgttca
     1261 tccctgtcca cgcccgcgac ttttcctgat agagctgcac gaacctgagt cccagggagg
     1321 cacgatatgt cgattccatc agtgaatctc caatcatcca tcacaggatc gcgccgccaa
     1381 ccatagcccg caaccactga gccatcgacc ggcgccgtca tggcctcggg catactgact
     1441 aagccctgct ccgattcgct gaggctgccg gagtcggccg cggatatggc attcaccccc
     1501 tcaatggagt cagccccaga ctcctcgtga ggtttgattg cgattgcctc atcggcctcg
     1561 ctttcaaaac ggcccgagtc tgtatggcct acgatttgag ccggcgtatc ccggcgaacg
     1621 ttgctcacta gtatggccaa ggagaatccg agaaagaaag cggctatccc aaccacgagc
     1681 atcatcgacg tagatctgct tccaaacggc catttcataa aatcacctcg ggttctaatt
     1741 cttccccgag gcctcatcca gtatgcttgc tgatctccag agcacgcgaa ctctcgataa
     1801 tgctcacatt acttgcggct tgagtcgagc ctctgtattt cgatgcccga gtagtaatgc
     1861 ttgagtatgg catcaaaagt caaacccgct ctggccaagt tttcagcccc ccactgacac
     1921 atccctacgc cgtgcccata gcctgtggtc gtgatagtaa ccttaccacc actagacaca
     1981 actgccaggc gcgttgactt gagtgagagc gctctccgca gatccgccgc ggatacagga
     2041 acaccataaa ccgacaccat actagccctg gccgtggcag ttctagccac tacctgaacc
     2101 cctgaagaca tcgcaggaag agacctgctt ccaagtccga gcgccttcac aagttctgca
     2161 ccctcaagcg tcttcatctc tccgagctcc aaagcttttt gctgacaccc gcaactcacc
     2221 ggaacaagat aggggacccg ccttccccag acagcggctg catcctctgt cattcctcca
     2281 cagcatgagt ggtagactgc gtctatgaga cgaccgttat atgtcatgac aagtccctcc
     2341 gtctcctcaa ccgcggcaat gacctttctt atgttagcca ggtatcctgc aactccccat
     2401 tttttcagaa gttcctccct agagctccat gcctggcaat gggatgggtc actgcagata
     2461 actgcgcctt tatgaacatc tgtcgaagct gaggtctcct gcgtctgcca catcttgcgg
     2521 agagtgtatg tccgggcagc cacggcttgt gccttcaatg cttcgagatc aaaagctgct
     2581 ggcatttctg cggcaaccac gcccacgaga tagtctgcca gtggcattcg ccgaactgtg
     2641 ccggccctac agtcccacag atcaacccag agagaagctg tagtgccagg cgccgtccta
     2701 ggacgcacaa acagagctat ggcgccaggc agtacaatta tgacaatgac gcatatagaa
     2761 accaaagacc acgcaaatct ggacacagac atgcatacct cccctgccgc gcacgaagct
     2821 gtacagtcta tgccctcatt gaaaccgtag aacagccgag ttccgtccga tacaatgctg
     2881 taaaaaggcc ttagccctgt tgtagtggat ggggatggta taaaaaggcc actggttctc
     2941 cagtagaatg caccaactat ctactaggag gagaaccaat ggctacatat cagattagta
     3001 tatccgaaga gcaattgcga ggacactctc tgcatgccag ttgcatccta caagcctcaa
     3061 catgctttta ccattttcga ccaaatggac cggactcgga aataacgcgg tcaatacata
     3121 atagtgactg ctaataccat acagaactct gccggccaag gcctccagca cataaaaaaa
     3181 gacgcctgca tcaagacgtt ctcagatatc cttctatagg tccaggtagt tgtgggtaca
     3241 gttcgcaagc cacggaaagc gtgacaatgc tatactgcct gttcggcttc ggcagacgca
     3301 acccgcacaa tgttcgcgcc aacgctcgcc agtttgcctt cgaagttctc atagccccgg
     3361 tcaatatggt atatgtctgc cacctcagta tccccttcag ctgcaagacc tgcaagaacc
     3421 agcgctgcgc cggccctgag atcggtacac ttcacctgtg caccaaggag cttgtcctgc
     3481 ccttcaacaa cagccgaacg cccatctatc ttgatgtttg cacccatccg catcagctct
     3541 gcaacgtgca tgaacctgtt ttcaaagacg gtctccgtta tcactgaaga tcccgagcat
     3601 atagtcatca atgccatgaa ttgggcttga agatcggtag ggaaccccgg gtaaggcatt
     3661 gtcttaatat ccacgcctct tggacgatcg ttcatctcca cgaacatcga cccgtcatcg
     3721 ttctcctcaa ctgtgacacc gcattcgcgg agtttcgcag tgatcggttt caagtgttcc
     3781 gacacgacgt tctctataac gagcgaccca ccggcaatcg caggagctat cataaaagtg
     3841 cccgcttcta ttctgtcggg aaccaccatg taagacgttg gagatagctc tggcacgcct
     3901 tcgatccgca gaacggtagt tccggctccc ctcacacgtg cacccatcga gttcagaaag
     3961 tttgcaaggt caacaatctc aggttcctga gctgcgtttt ctataatagt cgtcccagac
     4021 gccaacactg cagccatcat gatattctca gtagcaccca cactagggaa gtccaaataa
     4081 accctggccc cctccagtcg atccgccctg gcctctatct gcccatatcc catgtctatt
     4141 gtggcgccta gcgccgcaaa accctttagg tgaagatcga taggacgaga tcctatggcg
     4201 catccccccg gaagcgcaat cctggcacag cccatgcgtg caagaagcgg ccccataacc
     4261 aagaaagacg cccgcatcgc cctcaccaga tcgtagggag gctcaaacga cgaaatctcg
     4321 gtagcgtcaa caacgacctt gtttttcgac cttgatacac gagcgccgac agatgtgagc
     4381 acaccacaca tagtctcaac atcaagcaat tcaggaacat cttctaccgt agaagaacca
     4441 caggcaagca aggaagctgc tagtataggc aaagaggcgt tcttcgctcc ccccacgcgc
     4501 agcctgccgt gtagggactc accacctttg ataacgaaag aatccacttt acctccccca
     4561 attccaaagc aaacaggcat tctgccccct tgcgcagttt aacatcgggt ctatccagag
     4621 cacaaccggg aatatcttcc cggtactata tgggcataaa atcggaaggt cgttcgtcaa
     4681 cgagcccata atggtggagt attcccataa ctgttctgtg agcggcattt ccgtcaccat
     4741 aagggttagg cgcgcttgcc attctccgat attgttctgc atcctccaga agaatacctg
     4801 cttcttcccg aatctgctct ctacccactc caactagtcg caaagttccg gttcgaagcc
     4861 cctcaggcct ctcagttgtc cgtctgagca ccagcactgg cttgcccaaa gcaggtgctt
     4921 cctcctggag gcctccggag tcggtcatca ccatgtagca tgcttccatg aggcccacca
     4981 tgtcggggta gcttaccgga tcaacaagga aacacctttc agccccgcta agctcttcca
     5041 ttacggtctc ccttaccctc ggattgagat gaacaggcat cacaaccaca acatcgggat
     5101 agtccgccaa cacatcacgg atagccgcgc agatattccg caaaggttca cctatgttct
     5161 cacgccgatg agcagtgacc aagattagcc tcttgccctc gagatctagg gacgcgagct
     5221 tcggatcatc gtaccccgta cgactctggg caaccctcat tacagcatcg actacagtgt
     5281 tccccgtaac gtagatgttt tcgcggggga aattctcccg aagcaaattc tccaggtgat
     5341 cagcagtggg cgcaaagtgc agatcggcaa ggcagcccac aagccttcgg ttcatttcct
     5401 ccggaaaagg gtcatacttc tgatatgtgc gcaaaccagc ttctacgtga ccaatagcag
     5461 tcttgcaata gaatgaagca agcgctccaa cgaacgcagt ggaagtatcg ccatgaacaa
     5521 gagtaatgtc cggtttttca cggactaata cttcctccag gccacggagg cctctctctg
     5581 caatgtcgaa aagcgtctgg tcatcctgca ttatgccaag atcatagtcg ggtgaaatgc
     5641 caaacactga aagtacttga tccatcatgt cacggtgctg cgcggtccca actgtcacag
     5701 agctgatcat atccggatac ttcgaaagct cggcaactac gggcgcaagt tttatcgtgt
     5761 ctggccttgt gccaaaaaca gacagaacct taatccttcc catgaaacaa cctccgacat
     5821 accatcaatg agcaacgcaa acgtgcaaga cctctattcg tccatcttcc gacgtggccc
     5881 gaccttcata accccggact tctcacctac gatgattaga gcaccaaatg caacgaccgc
     5941 cccgcctagg ccaactcgag ggttcac
//
LOCUS       DAOSAI010000112         5944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8490_length_5944_cov_4.900832, whole genome shotgun sequence.
ACCESSION   DAOSAI010000112 DAOSAI010000000
VERSION     DAOSAI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8490_length_5944_cov_4.900832"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLM83_05085"
     CDS             <1..409
                     /locus_tag="PLM83_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOK70815.1"
                     /translation="DITTHGRSCAVMNPYYTVFFAPAIERQLRIVGDIYRRYGYISAD
                     IDTLFGRDLGIAVAEGMIDFSRKAGFPITLGELPGFTDSHIDRALAAAKDPQLESKLK
                     SMPVKMDASSVDTYMKPILEAAKTGDLSLIVNM"
     gene            complement(508..2067)
                     /locus_tag="PLM83_05090"
     CDS             complement(508..2067)
                     /locus_tag="PLM83_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70816.1"
                     /translation="MDGAYPYWGLAAAALGVYTLAMLGIGAWAGRQVKDAEDYIVAGR
                     KLGFPLLFGTVLATWICGGLFMGGMAESYKHGFQGAILDPVGPGLWLLVAPVFYCILM
                     RRARYMTVPDMISTRYSKHAGLICSLAIVVYELFWVCATVTAFGSILSTFLNWPLWVG
                     ILAGGLVLIVYTWQGGMWAVTVTDLVQMVLLCIGMIMMLGRVVDLAGGWHALIANAAT
                     KENALPFNVLPGSGGFYGWTGMWAWSYLISGVVAGLGANAAAQDYWERALSGRSIKTI
                     IWASATAGLVYLALGSIPYFIGIGAYKIAPSLSAEQVESLGPWLLVNYFSPAAATFIA
                     VAMAAAVMSSADSGLLALASVCGKNLLQLLNPKAGDGDVLKWTRRWVTIGGIISLVMA
                     LFFQEIFRVQIFGHAILAIAAFPMMTLGLFDRKSNNLGALTGTYLGMGVWIGFSLFLT
                     HVQGAGFWDASEIAMLPALIVSFATMFVVSRLTAAADPPKCLVDVDGNPLPTSGAVLS
                     CKALFRRGEIE"
     gene            2307..3347
                     /locus_tag="PLM83_05095"
     CDS             2307..3347
                     /locus_tag="PLM83_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70817.1"
                     /translation="MNRCAIASFTSSQTRQAHCGKSRAAIIAIIVLAIIPRCAIAAPQ
                     DIGPPAGMLEPVGPEEISDMLIQAVQQRGCQARIEEDYLLMTFPDGESHNLIFRDLRL
                     FLAYVAEEDWEEVIEVYVEIVFNSIEEIRSLVNSFQSLDDAICHLGVCLYPADYLVRF
                     PDEEPVYRRDIDGLITCLVWDFSDMSVTAEWKDIEKWDFDIDELFSTAMENTLAKYPV
                     TTVEASVLPGLNLIIVESAGHPISAHMLAIDRLPGAMGPYGALVSAPAHNVVFIYPIA
                     DEGVMLALMAMAEIANDAVHVALNPLSDQVFWHNGQTFVNIPYIMGKEFPEIDIYNMP
                     PEFLMLLLTLSN"
     gene            3458..4276
                     /locus_tag="PLM83_05100"
     CDS             3458..4276
                     /locus_tag="PLM83_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOK70818.1"
                     /translation="MTRASDNRKPHLKLEVANVSGSHYPQHHIKAMPDEISRYCFVPG
                     DHIRGLKIAERLDNAAQVSANRAIFVHTGTYKGVKMTVCSTGMGGPQAAIAMEELGML
                     GADTFIRVGSAGALQPGMGVGDIAIATAAYRDGGATRRLLPIPFPAAADFHVTRALYD
                     AANESGAVFHLGVVASTDIFYAPADPSFDKMLADAGAICIEMETDTVFILGHVRQFRC
                     GAIFVMDTGDSGVRPDATGPWADHAANTGFLEGEDLAIDIALEAMRRLATEDEK"
     gene            4454..>5944
                     /locus_tag="PLM83_05105"
     CDS             4454..>5944
                     /locus_tag="PLM83_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOK70819.1"
                     /translation="MNSLLVMVVTFALYILAYNLYGKRLGSKVFQLATKNITPAEKFN
                     DGVDYVPTNKFILAGHHFTSIAGTGPIVGPVLGVIWGWVPALLWVIFGSIFAGAVHDL
                     GALVLSMRHDGKSIGDITGTVINPRARTLFFTIIFFCLWIVIAIFALLMANLFVQYPQ
                     CVFPIWVEIPIAMGLSYYLYQKKGNLAVGSVVAIVLMYLTIWIGLYIPLELTNEVWLI
                     IIMVYIFFASTMPVQKLLQPRDYINALELLVAMVLLIIGIIVRPQPIVAPAVVHAPAG
                     APSMMPFLFITIACGAISGFHSLVSSGTSSKQIASEPHALAVGYGGMLLEAALATLVI
                     IAVCTGVSSETWLETYSNFAKIPTLPTFAEGGARIVGALGIPEAFALNILAVFMVSFA
                     STTIDTATRLQRYVISEFGEDFQIGWMQNKYIAALIAVVSAYLLAFRGSANVIWPLFG
                     ATNQLLAGLALLTVTVYLAENKQPTAYTAVPMVFMIIISTWGMIANL"
BASE COUNT         1494 a         1766 c         1489 g         1195 t
ORIGIN      
        1 ggacatcact acccacggac ggtcatgcgc agtaatgaac ccctattaca ccgtcttctt
       61 cgctccggcc atagaaagac agctacggat agtgggcgac atatacagac gttacggcta
      121 catttccgcc gatatcgaca cactcttcgg acgtgacctt ggaatcgccg ttgccgaggg
      181 catgatcgat ttcagcagaa aagccggatt cccaatcact ctaggagagc tgccagggtt
      241 caccgactct cacatagacc gggccctagc ggcagcaaaa gacccacagc tcgagtcgaa
      301 gctgaaaagc atgccggtga agatggacgc atcctccgtg gatacgtata tgaagcccat
      361 cctcgaggct gcgaagacgg gagacttaag ccttattgtc aacatgtagc ccagaacttg
      421 attggcgcgg gaggcggcca gtagatgccc ccgcgccaat atccaggcaa agcaaagacc
      481 attcctgata aaccgtctct tagatgctca ttcaatctct cccctgcgaa aaagagcctt
      541 gcaggaaagc acagcccctg aagtgggcaa tggatttccg tccacatcca caaggcactt
      601 gggcggatcc gcagctgcag tcagcctcga tactacgaac atcgtcgcaa aactgacgat
      661 cagcgccgga agcatggcga tctcagatgc atcccaaaaa cctgccccct gaacatgagt
      721 aagaaagaga gagaaaccga tccatacgcc catccccaaa taagtccctg tcaaagcgcc
      781 cagattgttc gatttgcggt caaagagtcc aagagtcatc attgggaaag cggcaatggc
      841 taagatagca tggccaaata tctgcacgcg gaaaatctcc tgaaaaaaga gggccatcac
      901 gaggcttatg ataccgccta tggtcaccca tctcctcgtc cacttcagaa catccccatc
      961 cccggcctta gggttcaaca gctgcaggag gttcttcccg caaaccgagg caagagcgag
     1021 caatccgctg tcggcactgc tcatcactgc tgcagccatg gcaacagcga tgaacgtggc
     1081 cgccgccgga ctgaagtagt tcacaaggag ccacggcccc aaagattcca cctgctcagc
     1141 gctgagcgaa ggcgctatct tgtacgcccc gatgcctatg aagtatggga tcgaacctag
     1201 cgccaggtag accaggcccg ctgttgcgct agcccagata atcgtcttta ttgatcgtcc
     1261 tgaaagagcc cgttcccaat agtcctgagc agccgcattc gcccccaagc cggcaacgac
     1321 gccggaaatc aggtatgacc aggcccacat cccggtccac ccgtagaacc cgcccgaccc
     1381 cggcaagaca ttgaaaggca aagcattctc cttagttgcc gcattggcga tgagcgcatg
     1441 ccagcccccg gccagatcca ccactcgccc cagcatcatg atcatgccta tgcaaagaag
     1501 gaccatctgg acaaggtcag taacggtaac cgcccacatc ccgccctgcc acgtgtagac
     1561 aatgagaact aatccgccag caaggatccc cacccatagc ggccagttta aaaaggtgga
     1621 caagatcgag ccaaacgcag tcacagtggc gcagacccaa aacagctcgt atactacaat
     1681 cgccagcgag cagatgagcc cggcatgttt gctgtatctg gtagatatca tgtcgggcac
     1741 agtcatgtac ctggccctgc gcatgagaat gcagtagaag acgggggcaa ccaaaagcca
     1801 caacccaggg cccactggat cgaggatagc cccctggaat ccgtgcttat aggattcagc
     1861 cattcctccc atgaacagcc cgccgcagat ccaggtggcg agcacggtgc cgaacagaag
     1921 cggaaaaccg agttttcttc ccgcaacaat gtaatcctcc gcgtccttca cttgcctgcc
     1981 cgcccacgcc cctatcccga gcatcgccaa cgtgtaaacc ccgagagccg ccgcagccaa
     2041 accccagtat ggataagctc cgtccattgc cgtttcctcc agtcatgata gtcgcgcccc
     2101 caaaaaagcc ggattgcttc ttccacatcc aagcggaaac aatcagatgc accgcagaag
     2161 ttcggaccat tctgccgacg tagacggcat caagattagc accaaatcag cgtgttgtga
     2221 agacgcgaat ttataggagt actgtaggaa ggagttgttg cccaaaccga agaaaggaga
     2281 ctcacccgat tataggaggg actcaaatga accgttgcgc catcgcctca ttcacgtctt
     2341 ctcagacccg tcaagcgcac tgcggcaaat cccgggcagc gatcatcgcc attatcgtcc
     2401 tggcaatcat cccccgttgc gccatcgccg ccccccagga cataggcccg cccgccggaa
     2461 tgctcgagcc tgtgggccct gaagaaatct ctgacatgct catccaagca gtacaacagc
     2521 gaggctgcca ggctcgcatt gaagaggact acttgctcat gaccttcccg gatggggaaa
     2581 gccacaactt gatcttccgg gatctgcggc ttttcctcgc ttatgtcgcc gaagaagatt
     2641 gggaggaggt catcgaggtc tacgtagaga tcgtcttcaa cagcattgaa gagatcagaa
     2701 gcctggtaaa cagcttccaa agcctcgatg acgccatttg ccacctaggc gtgtgcctgt
     2761 acccagcaga ctatctcgtg cggtttccgg acgaagagcc cgtataccgc cgtgatatcg
     2821 atggcctgat cacttgcctc gtatgggatt tctcagatat gagcgttaca gccgaatgga
     2881 aagatatcga gaaatgggac ttcgacatag acgagctctt ctccaccgcc atggaaaaca
     2941 cgcttgcaaa atatcctgtg accactgtcg aagccagtgt gcttcccggg ctcaacctca
     3001 tcattgtgga gtctgcggga caccccattt cagcccacat gcttgccatc gacagattgc
     3061 cgggcgcaat ggggccttac ggcgcactcg tcagcgcacc cgcccacaac gtggtcttca
     3121 tctacccaat agcagatgag ggcgtaatgc tcgccctcat ggcaatggcc gagatagcta
     3181 atgacgccgt ccatgtcgcc ttgaaccccc tgagcgatca agttttctgg cacaacggac
     3241 aaacctttgt taacatccct tatatcatgg gaaaagaatt tcccgagatc gacatataca
     3301 acatgcctcc ggaatttctc atgttgctgt taaccctgag caactaggca acaatatgcc
     3361 tgcacccgcc aagatcacca gatatgccgg gtatgaccgt ttgcaaaggg cggacatgcc
     3421 cggcacacat gtactgcaaa accgaggagg aacgagaatg acacgagcat cggacaatcg
     3481 caaaccccat ctcaagttgg aagtagccaa tgttagcgga tcgcactatc cgcagcatca
     3541 cataaaggca atgccagatg agatctcccg atactgcttt gtcccgggtg atcacataag
     3601 aggcctgaag atcgccgaac gactcgataa cgcagctcag gtcagtgcca accgcgccat
     3661 attcgttcac acaggaacct acaaaggagt gaagatgacc gtctgttcca cagggatggg
     3721 agggcctcaa gctgccatag ccatggaaga gctgggcatg ctcggcgcag acacctttat
     3781 cagagtgggc tctgccggcg ccttgcagcc cggcatggga gtgggagaca tcgcaatcgc
     3841 caccgcagcc taccgcgatg gaggcgcaac aaggagactc cttcccatcc cgttccccgc
     3901 cgcggccgat ttccatgtca cccgcgcact gtacgacgcg gccaacgaaa gcggcgccgt
     3961 tttccacttg ggcgtagtag cttccaccga catcttctac gcgcccgctg acccaagttt
     4021 cgacaagatg cttgctgacg cgggcgcaat ctgcatagaa atggagacag ataccgtatt
     4081 catcctaggc cacgttcgac aattccgctg tggcgccatt ttcgtcatgg atacaggaga
     4141 cagcggcgtc agacctgacg ccacggggcc atgggctgac catgcagcaa acaccggatt
     4201 cctagagggc gaagacctcg caatcgatat cgctcttgag gcaatgaggc gattggcgac
     4261 agaagatgag aagtagcgta aaggtaagac tcttccgtat caagggcttg ccatatcctc
     4321 catgaagtgc atgccaaatg gtatacatat gtagattgtt tagtaaatat agcaggaact
     4381 cggcagaaga tggcgaatac ctgtccgggc agcgcctgaa cgcgccccct aatgatactg
     4441 ggaggtggac taggtgaatt ctctgttggt gatggtagtt accttcgctc tgtacatcct
     4501 cgcctacaac ctgtatggaa agagactggg ctctaaagtg ttccagctcg ctacgaagaa
     4561 catcactcct gctgagaagt tcaatgatgg tgtggactac gtcccgacca ataagttcat
     4621 cctcgctggt caccatttca catcgattgc gggaaccggg ccaatcgtgg gaccggttct
     4681 cggcgtgata tggggctggg tacccgcgct tctgtgggtg atcttcggat cgatattcgc
     4741 cggagccgtc catgacctag gcgcattagt cctgtcaatg cgccacgacg gcaagtcaat
     4801 aggcgacatc accggaacag tcatcaatcc ccgtgcacgg accctattct tcacgatcat
     4861 cttcttctgc ctgtggatcg ttatcgccat attcgccctg ctgatggcga acctcttcgt
     4921 ccaataccca caatgcgtct tccctatttg ggtcgagata cccatagcca tggggctctc
     4981 gtattacctg taccaaaaga aggggaatct cgccgtcggc tccgtagtgg ccatagtatt
     5041 gatgtacctt acgatctgga tcgggctgta catcccgcta gaactgacca acgaagtctg
     5101 gctcatcata ataatggtct acatcttctt cgcatcaaca atgccagtcc aaaagctgct
     5161 acaaccgcga gactacatca acgcgctcga acttctggtc gccatggtac tcctcatcat
     5221 aggcatcatc gtaagaccgc aaccgatagt cgcgccggca gtggtccatg cgcctgcagg
     5281 agcaccgtca atgatgccat tcctgttcat aacgatagct tgtggtgcaa tatcggggtt
     5341 ccattctctg gtgtcgagcg gcacaagctc gaagcagata gccagcgaac cgcatgccct
     5401 tgcagtagga tacggcggaa tgctgctcga agcggccctt gcaactctgg taataattgc
     5461 tgtatgcaca ggcgtctcct ccgaaacttg gctcgagaca tactcgaact tcgccaagat
     5521 tcctacgttg cccacatttg ctgaaggcgg cgccagaata gtaggcgcac tcggaatccc
     5581 tgaggccttc gccctcaaca tcctggcagt cttcatggtg agcttcgcaa gcacgacaat
     5641 agacacggca acccgtctgc aacggtacgt aatctccgaa ttcggcgagg acttccagat
     5701 aggctggatg caaaacaagt acatcgcagc gcttatcgca gtagtcagcg cctacttgct
     5761 ggccttccgc ggcagtgcga acgttatctg gccactgttc ggcgcgacca accagctcct
     5821 cgccggactc gcattgctca cagtcacggt atatctggcc gagaataagc agcccacggc
     5881 atacacagca gtgcccatgg tattcatgat catcatcagc acatggggca tgatcgcaaa
     5941 cttg
//
LOCUS       DAOSAI010000113         5941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8495_length_5941_cov_5.154604, whole genome shotgun sequence.
ACCESSION   DAOSAI010000113 DAOSAI010000000
VERSION     DAOSAI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8495_length_5941_cov_5.154604"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1030
                     /locus_tag="PLM83_05110"
     CDS             194..1030
                     /locus_tag="PLM83_05110"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOK70820.1"
                     /translation="MTDSAEHDGTLQALCPHCGKGYRLKSSLLGKKAKCGACQQSFTI
                     AAAGNPEDDALYNIEDEAPPRPVPAVEIARPAPNTFAPPPPPAPPPPEIARPAPNTLL
                     ALEQLSSKLELALKGILGSDEPVYVKLNGGAKEGLICTDTRVILLKRAGFTSVSSFQA
                     AYGDIAGAEVKFHLLTGHFELSVIGMQTPSNNHWSEPNCILLNSRDQASKFREVCSFI
                     LRKRSQARSPNTSNDGHSPAMSRAVSDPTEQLERLAKLLEKGLLSEQEFRQQKQKILE
                     RM"
     gene            1102..2772
                     /locus_tag="PLM83_05115"
     CDS             1102..2772
                     /locus_tag="PLM83_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK70821.1"
                     /translation="MAASRQSDFATVFANLLKARFPYLYISTWEEERALAVIRSVAGD
                     SKLIKTPRDVFVWTLTKGLIGDSQTGAEETKAPLKVLDIIEKHPTPALFVLKDFHIYF
                     GGEGRPPDYQIVRRMRDIVASLKSSPKPKNVVLLCPALVLPNDLQKDVTIVDFDLPSF
                     PEIKGLLDDMIAANQKTGRIQINLTADETERLAKAALGLTLQEAENAFARAMVEDGRL
                     NIDDVEVILDEKCQIIKKTEILEFIKADLSMEDIGGLENLKRWLKKRDKSWLDSAQRY
                     RLPSPKGVLITGVPGCGKSLIAKAISAAWQLPLLRLDMGKIFSGLVGSSEENMRKAIK
                     TAEAIAPSVLWIDEIEKGFSGTSGNGDSGTSSRVFGSFLTWMQEKTKPVFVAATANNI
                     DCLPPELLRKGRFDEIFFVDLPTQRERTDIFLLHLRKRLKDPSVVGKFSVDVDSVTTL
                     AKQTEGFVGAEIEQVVISGLFEAFAEDRSVRLDDFQRAIRNTVPLSVTQAEQIRAIRN
                     WANVRAVAATTEEHRAEYSRPLEKSPSPKSEPVGEDIVASRGGRTVDF"
     gene            2793..3551
                     /locus_tag="PLM83_05120"
     CDS             2793..3551
                     /locus_tag="PLM83_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70822.1"
                     /translation="MSVSLVLIPLALPAIPILLGLRAALGKEGFQKLIDSIQVKVPTS
                     LASREQLVKVVTAAKLDIQPWAGGLKTHFRGTSQFFTWEFDGQRWYAVFAKPITQEMV
                     AQVINDIEGRSGERIFSAVTATPSSKVIAQSASIPTNFRDEALLLKVLIDAGAKPARD
                     AQGTIRCSFQGTTLRFVRGPSQVYEAIVQNAINADPVFQHLSTLEDDYRRCVQAATYQ
                     QIKASIADKNMTLESEDVLEDNSIVLTLNIQEHR"
     gene            3551..3763
                     /locus_tag="PLM83_05125"
     CDS             3551..3763
                     /locus_tag="PLM83_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HOK70823.1"
                     /translation="MAKRIKLRIFPNGSVQAEVQGVKGKACTDFIPVLEALLKAETVD
                     SAYTPEYYEGVSVEIQTGQQQHIQGV"
     gene            3767..4603
                     /locus_tag="PLM83_05130"
     CDS             3767..4603
                     /locus_tag="PLM83_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOK70824.1"
                     /translation="MSHPWTYWSFCPGFTCFSWLHAAIRSHNKTYLVYTAVYAIFLVL
                     MMCGPSVEQTYGTDSIANNIVMTFVHIAWIVGIVHTQWVKQSVSSSFPVDTNQSNAQA
                     AAGVPAAQTAQLAPLPQHQSSTGPVREWRCRIDGRKYGPFTTSQLRQMAQSGIIKPTD
                     EVFRDGATDWVRADRVKGLFPAPASANRSDPGGNQTGESVDLNNDPEEAIAALPGVGT
                     ILAKKAVSIRQSSGGFATVEAFAEALGLKPHMVERIRPLVKLGVRKLEVPPATKTRVV
                     DF"
     gene            4603..5235
                     /locus_tag="PLM83_05135"
     CDS             4603..5235
                     /locus_tag="PLM83_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HOK70825.1"
                     /translation="MVLRINRFLPFTRAEGPGNRACLWVQGCPIRCPGCAVPWTWPDD
                     GGQTVSVADLAEQILNGPEIEGVTFLGGEPFAQAKALASLGTIVKEAGLSIVTFTGYE
                     LDFLQGAQDDDLLDLLHITDLLIAGPFRRECLDLSRPWVGSSNKQFHFLTDRYSHIQE
                     HLQSIPNRMEIRLLPSGEVRVNGMFQPAHLAFLRDVLHPASNEHPIAHGS"
     gene            complement(5247..5849)
                     /locus_tag="PLM83_05140"
     CDS             complement(5247..5849)
                     /locus_tag="PLM83_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOK70826.1"
                     /translation="MSEFCTAAVFSFMMFMSAAFLLPGFREALAATPGAFAAYVYLSL
                     ILSGVSAAVWVVRGHIDRIAKAQAVADQEPQLMPSISSSIPDLSQKTTAPLEVRINIA
                     SETEIAALPTVGPILAKRIIQERTQRPFSSTDDLVSRCDLKPHQIVRIAPFIKFDAGE
                     ASWLCRVGKWFSGNTGSTEIPTHQAPQPTSEQHRGRIVDA"
BASE COUNT         1416 a         1607 c         1630 g         1288 t
ORIGIN      
        1 cggacaggtg taactcagga aggctggttc tccatttcag gctgaaccag gacaccatat
       61 atgtgaaagc acatcaggga cttaggtcgt aatcggttgg ctgctccaga cggcgacgtg
      121 cggtttcttt ggcgcgactc tagcagttgt gcacaagacg caaccgtttt ttgcttctga
      181 aactggtggg cacatgacgg attcagcaga gcacgacgga accctgcaag ctttatgccc
      241 gcactgtggc aagggctatc ggctgaaatc atcccttctt ggcaagaagg caaagtgtgg
      301 tgcctgccag cagagtttca cgattgccgc ggccgggaac ccagaggatg acgcacttta
      361 caacatcgaa gatgaggccc ctccgcgacc agttcctgcc gtcgagatcg cccgaccagc
      421 accaaacaca ttcgcacctc ctccgccccc tgctcctccg ccgcctgaga tcgcccgacc
      481 agctccaaac acactactag cccttgagca actatcatcg aagctcgaac tagcattgaa
      541 gggaatcctg ggatctgacg aacccgtgta cgtaaagctg aacggtgggg caaaagaagg
      601 gctcatctgt accgacaccc gagtcatcct gcttaagagg gcaggtttca cgagtgtcag
      661 tagctttcag gccgcctacg gcgatatcgc gggcgcagaa gtgaagttcc atctcctcac
      721 ggggcacttt gagctgtcag tcattggaat gcagactccg tcgaacaacc actggagcga
      781 gccaaattgc atcttgttga acagcaggga tcaagcaagc aagttccgag aggtgtgcag
      841 tttcatcctt cgaaaacgca gtcaggcccg ctcccccaat acgagcaatg acggccactc
      901 tcctgctatg tcgcgagcag tatctgatcc cacagaacaa cttgaacggt tggcgaagct
      961 tttggagaag ggcttgctct cggagcaaga gttccggcag cagaaacaaa agatcctgga
     1021 gcgaatgtga ttggctgaac tactgaggcg tctttgctaa ggggatgccc gcctcgggca
     1081 ttttgaaagg tcgtacagga catggcagca tcgcgacaat cagattttgc aaccgttttc
     1141 gcaaatcttc tgaaagcgag attcccatac ctgtacattt ctacctggga ggaggagcgg
     1201 gctctcgcag ttatacgctc tgtcgctggg gactccaagc tcattaagac cccgcgtgac
     1261 gtctttgtct ggactttgac caaagggctg atcggtgatt ctcagaccgg cgccgaagag
     1321 accaaagctc ccttgaaagt cttggacatc atcgagaagc atccaacgcc tgctctcttt
     1381 gtcctcaagg attttcacat ttactttggt ggagaaggca ggccaccaga ctatcaaatc
     1441 gtccgacgca tgcgcgacat tgtggcttcg ctgaagtcga gccccaaacc taagaatgtc
     1501 gtcttgctct gcccagcatt ggtcttgccg aacgacctcc aaaaggatgt gaccatcgtc
     1561 gattttgatc tgcctagctt ccctgagatc aagggtctcc tcgacgacat gatcgctgca
     1621 aaccagaaaa caggcagaat ccagatcaat ctcacagccg acgagactga acggctggcc
     1681 aaagcggctt tgggcctgac cctccaggaa gcagagaatg ctttcgccag ggcaatggtc
     1741 gaggatggac gtttgaacat tgatgacgtc gaggtcattc tcgatgagaa gtgccaaatc
     1801 atcaagaaga cggaaatcct tgagttcatc aaagcagacc tgagcatgga ggatattggc
     1861 ggcctggaga accttaagag atggctcaag aaacgcgaca agtcgtggct cgactcggcg
     1921 caacgataca ggctgccatc tccgaagggg gtcttgataa caggcgtgcc agggtgtggc
     1981 aagagtctga ttgcgaaggc aattagtgcc gcttggcaac ttccattgct gcgactcgat
     2041 atgggcaaga tcttcagcgg cttggtaggg agcagcgaag agaacatgcg gaaggccatc
     2101 aagactgctg aggcaatcgc accttcggtt ctttggatcg atgagataga gaagggcttt
     2161 agtgggacat caggcaacgg cgatagtgga acgtcaagcc gagtgttcgg cagtttcctc
     2221 acctggatgc aagaaaagac gaagcctgtt tttgtagccg ctacggccaa caacattgat
     2281 tgcttgccac cggaactcct caggaaaggc cggtttgatg agattttctt tgtagatttg
     2341 cccacacagc gcgagcggac tgatatcttc ttgttacact tgaggaaacg gctcaaagat
     2401 ccgagcgtcg ttgggaagtt ctctgttgac gttgattctg tgacgaccct cgcgaagcag
     2461 acagagggtt tcgtcggcgc ggagattgaa caggtcgtga tctctggttt gttcgaggcc
     2521 ttcgccgagg accggtccgt gcggctagac gacttccaga gggccatccg caacaccgtt
     2581 ccgctctccg taacgcaggc tgaacagatt cgggcaatcc gcaactgggc taatgttcgg
     2641 gccgtcgctg ccaccactga agaacaccga gccgaatatt cacgaccgtt agaaaagtca
     2701 ccctctccga agagtgaacc tgttggggag gacatcgtcg cgtcccgtgg tgggcgtact
     2761 gttgactttt gagcaggaca caggagcagt acatgtccgt ttcactagtc cttattccac
     2821 tggcacttcc tgccatccca attctactcg gcttacgagc ggcgctcggc aaagagggct
     2881 ttcagaagct gatcgattcc atccaggtga aggtacccac cagtctcgca tctcgcgaac
     2941 aactggtgaa agtcgtaact gcggcgaaac tcgatattca accctgggca ggtggcctca
     3001 aaacgcactt tcgcggaaca tctcagttct tcacctggga gttcgatggg cagcgatggt
     3061 acgcagtgtt cgcaaaaccc atcacccagg agatggtcgc ccaagtgatc aacgatattg
     3121 aaggccgatc gggagagagg atcttctcgg ccgtgacggc tacaccttcc agcaaggtaa
     3181 ttgcccaaag tgcttccatc ccgacgaact tccgcgacga agctctgctt ttgaaggtcc
     3241 tcatcgatgc tggtgcaaag cctgcacggg atgcacaagg aacaatcagg tgctcttttc
     3301 aaggaactac gctgcggttt gttcgaggcc cgagccaagt atacgaggcg atagttcaaa
     3361 acgcaatcaa cgcagatcct gtgttccagc acctaagtac gctggaagac gattaccgcc
     3421 gctgcgttca ggccgccaca taccaacaga tcaaggccag cattgccgac aaaaacatga
     3481 cgctggaatc ggaggacgtc ctggaagaca actcgatcgt gctcacactc aacatacagg
     3541 agcacaggta atggcaaagc gcattaagct acggatcttc cccaatggca gcgtgcaggc
     3601 ggaggtgcaa ggggtgaagg gcaaggcttg cacggatttt atcccagtct tggaagctct
     3661 cttgaaagcc gaaacggtag attcggcata cacacctgag tactacgaag gcgtgtctgt
     3721 agaaatccag actggccagc agcagcacat tcaaggagta taggctatgt cgcatccctg
     3781 gacctactgg agcttttgcc ctggcttcac ctgcttttca tggttgcatg ctgccatccg
     3841 ttcccataac aagacgtatt tggtgtacac cgcagtctat gccatcttcc tagtcctgat
     3901 gatgtgcggg ccgtccgttg agcagacata cggaacggac tcaatcgcca ataacatcgt
     3961 catgacattc gtacatatcg cttggatcgt cgggatagtt cacactcaat gggtcaagca
     4021 gagtgtatcc agttccttcc ccgttgacac caatcaaagc aatgctcaag ccgcagcggg
     4081 cgtacccgcc gcccagactg ctcaactggc tccccttccg cagcaccaat caagcacggg
     4141 gccggttcga gagtggcgtt gcaggattga tggccgaaag tacggcccct tcactacctc
     4201 gcaactgcgc cagatggccc agagcgggat aatcaagccg accgacgagg tatttcggga
     4261 tggtgccaca gactgggtac gagcagaccg agtaaaaggc ctgtttccag caccagcttc
     4321 cgccaataga agtgatccag gcggtaatca gactggagaa tctgtggacc tcaataatga
     4381 tccggaagag gcaatcgccg cgctgccagg agtcggtacg atattggcca agaaggccgt
     4441 gagtattcga cagtcttcgg gtggcttcgc gacggttgag gcatttgcgg aggcacttgg
     4501 gctgaagccc catatggtcg aacgaattcg tcccctcgtg aagctcggtg tgagaaaact
     4561 cgaggtgccc cccgcgacaa aaacacgggt tgtcgatttc taatggtcct tcgaatcaac
     4621 cggtttcttc ctttcaccag ggccgagggg ccgggcaatc gcgcctgtct ctgggtgcaa
     4681 ggttgtccca tccgatgccc cggctgtgcc gtgccctgga cgtggcctga tgacggtgga
     4741 caaacagtgt ctgtcgccga cctagccgag caaatcttga atgggcctga gattgaaggc
     4801 gttacctttc ttggcggcga gccatttgcc caagcgaagg cactggcgag ccttggaacg
     4861 atagtcaagg aggcgggact ctcgatcgtg accttcacgg gctacgaact agatttcctg
     4921 caaggtgccc aggatgatga cttgctcgat ctgctgcata tcactgacct tcttatcgcg
     4981 ggtccgttcc gacgagagtg cctcgacttg agccgtcctt gggttggctc atcaaacaaa
     5041 caattccatt ttctgacgga tcgatacagc catattcagg aacacttgca gagcataccg
     5101 aaccgaatgg aaattcgact cctcccgagc ggagaagtgc gagtcaatgg aatgtttcag
     5161 cctgctcacc tggcttttct cagggatgtg ctgcaccccg cctctaacga gcatccgatt
     5221 gcacatggct cctaatatcg aagacgttag gcgtcaacaa ttcgccccct gtgctgttcg
     5281 gacgttggct ggggcgcctg atgagtaggt atctctgttg aaccggtgtt cccgctgaac
     5341 cactttccca ctctacacaa ccaggatgcc tctccagcgt cgaactttat gaagggagca
     5401 atcctcacta tttgatgcgg cttgagatcg caacggctca ccaggtcatc agttgaagag
     5461 aacggccttt gtgtcctctc ctgaatgatg cgcttggcca gaatcggtcc taccgttggc
     5521 agtgcggcga tttctgtttc gcttgcgatg ttgatgcgga cctctaaggg ggcggtggtc
     5581 ttctgagata gatccggaat gctgctgcta atggatggca ttagctgcgg ctcttggtcc
     5641 gccactgcct gcgctttcgc aatccggtcg atgtgtcccc tgactaccca gacggcggcg
     5701 gaaacgccgg aaagaataag agacaaatag acgtatgcag caaaggctcc tggggtggca
     5761 gccaaggctt ccctgaagcc gggcagcaga aatgcagcgc tcatgaacat cataaagctg
     5821 aagactgcgg cggtgcagaa ctcagacatc tgacggcccc gcttcccgcc taaacaggct
     5881 tgacactgag atataaccaa ttcttgtgga tggcgtgaat aaaaaggcgg cgtatcctgg
     5941 g
//
LOCUS       DAOSAI010000114         5857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8650_length_5857_cov_3.574457, whole genome shotgun sequence.
ACCESSION   DAOSAI010000114 DAOSAI010000000
VERSION     DAOSAI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8650_length_5857_cov_3.574457"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLM83_05145"
     CDS             complement(<1..266)
                     /locus_tag="PLM83_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70827.1"
                     /translation="MGKDRLGESCVREAYEPRKYRELFSAQDVIYFRCAVGQTDLQVG
                     VGFSCPDFRGKLPEESFLRDHVSGVVLRARRDIENYIATHPEFLT"
     gene            complement(277..735)
                     /locus_tag="PLM83_05150"
     CDS             complement(277..735)
                     /locus_tag="PLM83_05150"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK70828.1"
                     /translation="MTVERTVGADSITDATTAKRVKALLTYPPGAAGKPITYSLIREF
                     DLWINILHAEIAADKAGKLIMDIQGSVEDVDRGLEFLDEEGIGYEILNGSIVWDEDKC
                     VHCGACTAVCPSHALTMDNCDWSLVFDKEKCYLCRLCARTCPVGAMSSPI"
     gene            complement(738..1901)
                     /locus_tag="PLM83_05155"
     CDS             complement(738..1901)
                     /locus_tag="PLM83_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOK70829.1"
                     /translation="MGYRSFEEINEKIRSKKAVVVTAEEIIEIVDEKGVEGAFRDVDV
                     VTCGTFSPMCSSGAFLNFGHSDPPIRMSRVWLNDVPACAGLAAVDAYIGATELSETAG
                     MEYGGAHVIEDLIAGRKVRLKATSYGTDCYPRRALEAWITKDSINEAYMFNPRNCYQN
                     YGAATNSSDRTIYTYMGTLLPHFGNVNYCTAGELSPLINDPFYRTIGLGSRIFLGGAQ
                     GYVAWQGTQNNPSQPRAENGVPIGGAACLALIGDLKQMDPRYIRAARFEKYGVTMFVG
                     VGTAIPLLDEEMVRFVAVQNRDIKTKVFDYSVGRRSKPALAEVSYEELRSGSVQIGSR
                     TIPTAPLSSLKVAREIAQTLKGWIARGEFLLQQPVAALPLEGSPTTLDNCEGE"
     gene            complement(1902..4364)
                     /locus_tag="PLM83_05160"
     CDS             complement(1902..4364)
                     /locus_tag="PLM83_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOK70830.1"
                     /translation="MSVRVEDLIRGDEVFVFDGAFGTMLQARGLPPGACPEVWCLKCP
                     EEVERVHRQYIDAGAQVIETCTFGGTALRLGHFGLAHAAREINLAAVEIARRAAADRG
                     TLVAASMGPLGALVEPLGEVSFDEAYLQFAEQAEAFALARPDLIIIETIADLNEMRAA
                     LLACRDRAPDIPVIAQMTFDSTGRTFTGTPPESAALVMESMGASVVGVNCSVGPDLLA
                     DVVARMGAVTSKPISVQPNAGLPHVGPSGRGEYPMGPEEFASYGPKLVSAGASIVGGC
                     CGTTPEHIARLRSALEGLRPIVRRREPELGITSRTQPLFFADAELPAIVGERINPTGR
                     RKLAQDIVNGSFELVRKEAVAQAKAGAQALDVNVGTPLANEPWAMYEAVKAIEATTRL
                     PLCLDSSSAEALEAGLRAYAGRALVNSFSLERGREEDVLRLAARYGAAVIGLPIDENG
                     IPATAEDRLRLAERLVSSAQSHGIPQNAVIIDALAMPCGAKQEQAVETLKAIEMIRKE
                     LGCKTSLGISNVSFGLPSKPVLNTAFLMLAIAAGLDMAIANPLDEAMMAQVRAARVLL
                     GRDVNAERYILWAGAASPAPHGVAADPGGKKLTLVYDAVLGGDKDLAVRLVEEAVSEG
                     EDPSSILNDALIPAIEETGRRFASGAYFLPELMLSAGAMQAATEVIRSKVGSAEDAPA
                     SRGTVVIATVEGDIHDIGKNIVSMFLESNGYNVVDLGKNVKARDVLAGAKKAGADVVC
                     LSALMTTTAPKMRESIELFSSEGYDCPIIIGGAATSREYAIRIGADGYGSDAVEAVFE
                     VERLLARKRAFGRQSDQVGGKE"
     gene            complement(4361..5296)
                     /locus_tag="PLM83_05165"
     CDS             complement(4361..5296)
                     /locus_tag="PLM83_05165"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOK70831.1"
                     /translation="MARLLNRLRQGSFAVTVEVNPPKGADVSKTLALVRRFSDRVDAV
                     NVADCPMANVRMSPITLAHLIQRDVGVEAIFHLTCRDRNTIGLQAELLGASGLGVRNI
                     LALRGDPPSRGDHPEAHGVFEVDAVGLISLAAALNSGHTIGGGELGEGTDFAIGCVAN
                     PNAEDLEAEISRLEEKIAAGAHFVQTQPIFDVRAYERWANAIDGRVSIPILYGILPIK
                     DAAFAVHLKENVPGMEVPDWVIDRMRSGGDGEGYRLAAELLSEIGKDIAGIHIFPMNS
                     SKRVVGVLDALAEEGFAVPPVQTACDGLRKVAPNG"
BASE COUNT         1198 a         1915 c         1502 g         1242 t
ORIGIN      
        1 gtgagaaact cggggtgagt ggcgatgtag ttttcgatat ctctgcgggc gcgaagaaca
       61 acgcccgaga catgatcccg aagaaagctc tcttcgggaa gtttcccgcg aaaatcaggg
      121 caagagaagc caacgccgac ttgaagatca gtctgcccca cggcgcatct gaaatagatt
      181 acatcttgag cgctgaaaag ctccctgtat ttcctcggtt cgtaggcttc tctcacgcaa
      241 ctttccccca acctatcttt gcccatcccg ccccctctat atgggcgagc tcattgcccc
      301 cacgggacat gtgcgggcgc acaaccggca aagatagcat ttctctttgt caaatacaag
      361 gctccaatca cagttatcca tagtgagcgc gtgcgaaggg catacagccg tgcatgctcc
      421 acaatgcacg cacttgtcct catcccaaac gatgctgcca tttaggatct cgtacccaat
      481 gccctcttcg tcgaggaact ccaagccccg atccacatct tcaacgcttc cctgtatgtc
      541 catgatgagc ttgccggctt tgtccgcggc gatttcggcg tggagaatat tgatccacag
      601 atcgaactcg cggatcaggg agtaagttat gggcttgcct gccgcgcccg gagggtaggt
      661 cagcaacgcc ttaactctct ttgccgtcgt cgcatcagtt atgctatcgg ccccaacagt
      721 cctctccacg gtcatcctta ctccccctca cagttatcta gtgtagtcgg cgagccttcg
      781 agcggaagag ccgccacagg ctgctgcaac aggaactctc ctcgggcaat ccagcctttc
      841 aacgtctggg ctatctcccg cgccaccttc aggctggaaa gaggcgcggt agggatcgtg
      901 cgcgacccaa tctgaacact cccgctccgc agttcctcgt aggagacttc ggccaatgca
      961 ggtttggatc gacgaccgac gctgtaatca aagaccttcg tctttatgtc gcggttttgc
     1021 accgcgacga atctgaccat ctcctcgtcc agcagaggaa tggcagtgcc cactccgaca
     1081 aacatggtca ccccatactt ttcgaacctg gcggccctta tgtatctcgg atccatctgt
     1141 tttaggtcgc ctataagagc gaggcatgcc gctccgccga taggaacccc gttttccgcc
     1201 cgaggctgcg acgggttgtt ctgggttccc tgccacgcaa cgtacccttg ggcgcctccg
     1261 aggaatatcc gtgaacccaa cccgatcgtg cggtagaacg ggtcgtttat caaagggctg
     1321 agctcgcctg cagtgcagta gttcacattc ccaaagtggg gcaggagtgt tcccatgtac
     1381 gtataaatag tgcggtccga ggaattggtc gccgccccgt aattctggta gcagttgcgg
     1441 gggttgaaca tgtaggcctc attgattgaa tccttggtaa tccatgcctc gagcgcgcgc
     1501 cgaggataac agtctgtccc gtaggaagtg gccttaagcc tcacctttct gcctgctatg
     1561 aggtcttcga tgacgtgcgc tcccccgtat tccatccccg cggtctccga gagctccgtt
     1621 gcaccgatat aggcgtctac agcagcaagc ccagcgcatg ccggcacgtc atttagccac
     1681 acgcggctca tccttatggg aggatcagag tggccgaagt tgagaaaagc ccctgaggaa
     1741 cacatcgggc tgaaagtgcc gcaagtcacg acatccacgt cgcgaaaggc cccctctacc
     1801 cccttctcgt ccacaatctc tattatctcc tcagcagtca caacaaccgc ctttttgctg
     1861 cgtatcttct cgtttatctc ttcaaagctc ctgtagccca tctactcttt ccctcccact
     1921 tgatcgcttt gccgcccaaa agcacgcttt cttgccaaaa gcctttctac ctcgaagacc
     1981 gcttccacgg catcactgcc gtatccgtcc gctcctatgc gtatggcgta ctcccgtgat
     2041 gtagctgcgc ctccaatgat gattgggcaa tcgtacccct cgcttgaaaa aagctctatc
     2101 gactcgcgca tcttcggagc cgtcgttgtc atcagcgcgc tcaggcagac cacgtcggcc
     2161 cctgccttct tggcccctgc cagaacatcc ctggccttaa cgttctttcc caggtctact
     2221 acgttgtagc cgttgctttc gaggaacatg gagacgatgt tcttgcctat gtcgtggata
     2281 tccccttcga ctgtggcaat caccacagtc cctcgggatg ccggcgcatc ctctgcgctg
     2341 cctaccttgg acctgataac ttcagttgca gcctgcatcg cccctgccga cagcatcagt
     2401 tcagggagga agtaagcgcc tgaggcgaag cgccgtccgg tctcttctat agccgggatg
     2461 agcgcgtcgt ttaggataga agacggatcc tctccctccg aaaccgcctc ctccacaagc
     2521 cttacggcga ggtccttgtc gcccccgagg accgcatcat agaccaatgt caacttcttc
     2581 ccgcctggat cggcagcaac gccatgaggt gcaggcgacg ccgcgcccgc ccacaagatg
     2641 tatctttccg cattaacatc ccgcccgaga aggacccttg ccgcccgcac ctgagccatc
     2701 atcgcctcat caagggggtt agctatagcc atgtctaacc cggcagcgat ggccagcatc
     2761 aagaatgcag tgttcaacac gggcttgctc ggaagcccga acgacacatt ggaaatcccc
     2821 agagaagtct tgcagcccag ctctttgcgg atcatctcta tggccttgag agtctccaca
     2881 gcctgttcct gctttgcccc gcaaggcatc gccaaagcgt cgatgatcac cgcattttgg
     2941 gggattccat gagattgtgc gctcgagacg agccgctcag ccagtctcag ccggtcctct
     3001 gccgtcgccg gaatcccgtt ctcgtcgatt ggcaagccta tcaccgccgc gccgtatctg
     3061 gctgcaagcc tcagcacgtc ctcttccctg ccacgctcca gcgagaagga gttgaccaag
     3121 gctcggcctg cataagcccg aagccctgct tcgagagcct cggcagatga agagtcgagg
     3181 cagagcggca gacgagtggt cgcctcaatc gccttcactg cttcatacat cgcccacggc
     3241 tcgttcgcaa gaggcgtgcc cacattcaca tcgagagctt gcgctcccgc cttagcctga
     3301 gcaaccgctt ccttccgaac tagctcgaag ctgccgttga caatgtcttg ggcaagtttg
     3361 cgcctgccag ttgggttaat cctctcgccc acaatcgcgg gcagctccgc atcggcgaaa
     3421 aacagaggct gagtccggga agttatacca agctcaggct cgcgccttcg cacgatcggc
     3481 cggagcccct ccagcgcact gcgaagcctt gcgatgtgct ccggggtagt tccgcagcag
     3541 ccccctacga tagaggctcc cgccgacacc agcttagggc cgtaggacgc gaattcctca
     3601 ggccccattg gatactcgcc ccgccccgat gggcccacat gcggcagtcc agcattgggc
     3661 tggaccgata ttggctttga tgtaacagcc cccatccgag ctacaacatc tgcgagcagg
     3721 tccgggccga cagagcagtt gacgcccacg acagacgccc ccatcgactc catgacgagc
     3781 gcggccgact ccggcggagt gccggtgaag gttcggcctg tggaatcgaa tgtcatctgg
     3841 gcgattaccg ggatgtctgg cgcacgatcg cggcacgcga ggagcgctgc gcgcatctcg
     3901 ttgagatcgg caatggtctc gatgatgata aggtccggtc gcgccagggc aaacgcctcc
     3961 gcctgctccg caaattgcag ataggcttca tcgaaactga cttcgcctaa aggctccacc
     4021 aatgccccca gggggcccat ggaagccgcc acaagcgttc cgcggtcggc agcagccctc
     4081 cgggcaatct cgacggcagc caggttgatc tcccgcgccg catgagctag cccgaaatgt
     4141 ccgagccgca aagctgtgcc cccgaaagtg caagtctcga tcacctgcgc gccggcatca
     4201 atatactggc ggtgaacccg ctccacttct tccgggcact ttaggcacca cacttcagga
     4261 caggctccgg gaggcagacc gcgcgcctga agcatcgtcc caaatgcccc gtcaaacacg
     4321 aacacctcgt ccccacggat cagatcctcc acacgcacac tcatccgttc ggagccacct
     4381 tccgcaggcc gtcgcatgcc gtttgtaccg ggggcaccgc aaacccctcc tcggccaagg
     4441 cgtccagcac gcctacaacc cgcttggaac tgttcatggg gaagatgtgt atcccggcga
     4501 tatccttccc tatctccgat agcaactccg cagccagcct gtacccttca ccgtctcccc
     4561 cggagcgcat ccggtcgata acccagtcgg gcacctccat gccaggaaca ttctccttta
     4621 ggtgcacagc aaaggcagca tctttgatag ggagtatccc gtagaggata gggatgctca
     4681 cccgcccgtc tatggcatta gcccaccttt cataggcgcg gacgtcgaaa atgggctggg
     4741 tctggacgaa gtgcgctcca gctgcgatct tctcctccag cctcgatatc tcggcctcaa
     4801 gatcttcggc gttgggattt gctacgcaac cgatggcgaa atcggtccct tcacccagct
     4861 cgccgccgcc tatcgtgtgt cccgagttga gcgcggcggc caagctgatt agcccaacag
     4921 catctacctc gaacactcca tgggcctccg gatgatcccc cctcgacggc ggatcgcccc
     4981 tgagggccag tatgttcctg actccgagcc cggaggcacc caaaagctcc gcctgaagcc
     5041 ctatggtgtt gcgatcacgg cacgtaaggt ggaaaatggc ctctacccct acatcgcgct
     5101 ggataaggtg ggctaaggta atagggctca tcctcacatt tgccataggg cagtccgcca
     5161 cattcacggc gtccacccgg tcagaaaacc ggcgaaccag cgcaagcgtc ttggaaacat
     5221 ccgcaccttt cggggggtta acctccaccg taactgcaaa gctgccctgc ctcaacctgt
     5281 tcaataatct tgccacaaaa actccctcct gcgcgagctt tctctactta tctctgctga
     5341 aaagtcttta gccgcccaag ccaagaaggg caaatgcaaa aagccctctc ccccaggcaa
     5401 aatgcctggg acgagaggac taatccttcg tggtaccacc caggttcacg cggttctcac
     5461 gataccgcgc ctcggcaggt gcggcgaatc catccgattc gctagacccc ggcgctttaa
     5521 cgtgcgcaca cgtccgcgcc tactttccct ccctgcccat caaggagcaa agcggaggaa
     5581 gttcggacgg ccgctcaggg gccatgttcg tcgcatttcc gcggttccgg ctttcagctc
     5641 gaggccggct ctctgtgccc ggtactgtgc gagtactcat ccccgtcatt gcgtttagct
     5701 gcgattttaa acttggagac agtatatacc cccgtgtagc ggatgtcaag gttgggtttt
     5761 caatgaggca tttgcgttgt agccgatttc tcgcgcatgc cagttgcata atttgtcaaa
     5821 agcagcttcc tccctggcaa tgaagcagtc tcatttg
//
LOCUS       DAOSAI010000115         5836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8702_length_5836_cov_4.348902, whole genome shotgun sequence.
ACCESSION   DAOSAI010000115 DAOSAI010000000
VERSION     DAOSAI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8702_length_5836_cov_4.348902"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLM83_05170"
     CDS             <1..556
                     /locus_tag="PLM83_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOK70832.1"
                     /translation="APSCTPAELCTIVFVIDRASYDYFHHVAVSRRAVRLEEIVATMA
                     DLTNIQICMRLRAIGKDACFASDVLVPHGTLDAGALLGVYEKPDSEFVAYFRDTPYAR
                     LVEDAMNAWLANGSLVALENLAEGYLFRKIAPEREAVVGYETALAYLGARELEADKLR
                     RIFVGKMNKIPPNVIRERLSGAYA"
     gene            549..878
                     /locus_tag="PLM83_05175"
     CDS             549..878
                     /locus_tag="PLM83_05175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOK70833.1"
                     /translation="MHKIAVIGDEDSILGFRALGVSVFPAKNGKEAEEALRAAARRDY
                     AVIYITETHASEIIDVITEYSQAPLPVVLTIPDTRGGQGAAMEKIRRTVEKALGADIL
                     FGREGNA"
     gene            880..2664
                     /locus_tag="PLM83_05180"
     CDS             880..2664
                     /locus_tag="PLM83_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOK70834.1"
                     /translation="MNRGTSVGTIVKVSGPLVVASGMAGAKMYDVVRVSEQRLIGEII
                     EMREDRASIQVYEETAGLGPGEPVYITGEPLSVELGPGLIGSIYDGIQRPLDRIRDMA
                     GDRISRGLEAPGLDREREWDFVPRAGVGSKVVPGDILGTVQETPVVEHRVMVPPGISG
                     KVIWIHKGVAKVTDVIAKIETSDGVREIQMMQRWPVRHGRPIAHKLTPSEPLVSGQRV
                     LDTFFPVAKGGTACIPGPFGSGKTVVQHQLAKWADAEIVVYVGCGERGNEMTDVLMEF
                     PELLDPQSGEPLMKRTVLIANTSDMPVAARESSVYTGITIAEYFRDMGYSVALMADST
                     SRWAEALREMSGRLEEMPGEEGYPAYLGSRVSEFYERAGRTTCLGSDGREGALTVVGA
                     VSPPGGDLSEPVTQTTLRVVKVFWALDANLAYSRHFPAINWLTSYSLYLDRIDESLRK
                     MVGSEFVEMRQEAMKILQEEASLSEIVRLVGMDALSPGERITMETARSIREDFLHQNA
                     FHDVDTYTSLKKQAAMLRVILEMDHACRDAVEKGASFGQLAELAVHGKIARMKYIEEA
                     KISEIDELALEVRRDVAALTAGGGEDVA"
     gene            2657..4060
                     /locus_tag="PLM83_05185"
     CDS             2657..4060
                     /locus_tag="PLM83_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOK70835.1"
                     /translation="MLKEYLTVREVAGPLMLVEKVDEAKYGELVEIVVGGGEIRRGRV
                     LEVEGTNALVQLFEGSAGLELTSARVRFLGRGLELGVSPEMLGRVFDGLGRPMDDGPK
                     IIPEARLDTNGSPINPYARDYPSEFIQTGISAIDGLNTLVRGQKLPIFSGAGLPHSQL
                     AAQIARQARVLGTGEQFAVVFGAMGITFEEADFFISDFRRTGAIERAVLFINLADDPA
                     IERISTPKMALTAAEYLAYELDMHVLVILTDMTNYCEALREVSAARKEVPGRRGYPGY
                     LYTDLATLYERAGRIKGKKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIIVSRELH
                     RKGIYPPIDVLPSLSRLKDKGIGEGKTREDHADTMNQLYAAYARGKEAKELAVILGEA
                     ALSPSDKIFSKFADRFEQEYVRQGVDENRSIEETLDIGWDLLAMLPVDELSRIRMEFI
                     EKYLPKEAEKDAEPARE"
     gene            4069..4710
                     /locus_tag="PLM83_05190"
     CDS             4069..4710
                     /locus_tag="PLM83_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOK70836.1"
                     /translation="MEIRVSPTRMQLLQLKKSHAMAVRGHKLLKDKLDELMKQFLELV
                     KRDRELREEVDEKLGEAFRGFLIARGAMSSSAVDAALMYPKEQISLKASRRHIMNIEV
                     PEFEWSFEGQEDRTNIYPYGFAYTSPELDTAIRLLSEAMPSLVELAEVEKSVELLAGE
                     IEKTRRRVNALEHVVIPQQEASIKYITMKLDEAERANITRLMKVKEIVGERSV"
     gene            5001..5198
                     /locus_tag="PLM83_05195"
     CDS             5001..5198
                     /locus_tag="PLM83_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70837.1"
                     /translation="MSAPTLPVLGVIEIQRTTAKHAYNEDGNEPAPRFGKPVTDHVAD
                     IVDIWLLEDQLLSRKERGNTR"
BASE COUNT         1362 a         1383 c         1815 g         1276 t
ORIGIN      
        1 cgcgccttcc tgtactccgg cggaactatg taccattgtt ttcgtcattg accgcgcatc
       61 ttacgactac ttccaccatg tagctgtctc tagacgggct gtgcgcttgg aggagatcgt
      121 ggcgaccatg gcggatctca ctaacattca gatctgtatg aggctgcgtg ccataggcaa
      181 ggacgcctgt ttcgccagtg acgttttagt cccccacggg accttggacg ccggggcgtt
      241 gctcggagta tatgaaaagc ctgatagcga gtttgtggcc tatttcaggg atacgcccta
      301 tgcccggctg gtcgaggatg ccatgaacgc ttggcttgcc aatgggtctc ttgtcgcgct
      361 ggagaacctg gccgaaggct atctttttag gaagatagcg cctgagcgag aagctgtagt
      421 gggttacgag actgcccttg cctaccttgg cgcaagagag ctcgaggctg ataagctgcg
      481 ccggattttt gttggcaaga tgaacaagat cccgccgaat gtgatccgtg agaggctgag
      541 tggtgcgtat gcataagatt gcggttatcg gggacgaaga ctccatcttg ggcttcaggg
      601 ctcttggtgt atcggtcttc cccgcgaaga atggcaagga agcagaggag gcgctccgcg
      661 cggcggcgcg ccgtgattat gctgttatat atattactga aacccacgcg agcgagatca
      721 tcgacgtaat tacagagtac agccaggcgc cgctgcctgt agtgctcacc atacctgata
      781 cccgaggggg acagggagcg gccatggaaa agataagacg gacggtggag aaagcgctcg
      841 gcgccgacat cttgttcggg agagagggga atgcctgata tgaaccgtgg aacgagcgta
      901 ggaactattg taaaagtatc ggggcctctg gttgtcgctt cagggatggc gggggccaag
      961 atgtacgatg tcgtgcgcgt ctccgagcag cgactgatag gcgagatcat tgagatgaga
     1021 gaggaccgcg cttcgataca ggtgtatgaa gagaccgctg ggcttgggcc cggggaacca
     1081 gtctatatta ccggagaacc tctctcagtg gaactgggcc cgggactgat agggtccata
     1141 tacgacggca ttcagagacc gctcgaccgt atcagggata tggcgggaga tcgcatatcc
     1201 cgcggactgg aagctccggg actcgatcgg gagagagaat gggactttgt tccgagagca
     1261 ggcgttggct caaaggtggt tcccggggac attctcggca cagtgcagga gacgcctgtt
     1321 gtggagcaca gggttatggt tcctcccgga atttcgggca aggtaatatg gattcacaag
     1381 ggcgttgcca aagtaactga cgtaatcgcc aagatcgaaa cgtccgatgg agttcgcgag
     1441 atccaaatga tgcagagatg gcctgtgaga catggccgcc cgatcgcaca caaactgaca
     1501 ccgagcgaac ctcttgtgag tggacagagg gttcttgaca ccttcttccc tgtcgccaag
     1561 ggcggcacgg cgtgtattcc cgggccgttc ggatcgggga aaacagttgt ccagcaccag
     1621 ctcgccaagt gggctgatgc ggagatagtg gtttatgttg ggtgtggtga gcgcggtaac
     1681 gagatgacag acgttttgat ggaatttccg gagctgctcg atccacagag tggcgagccc
     1741 ctcatgaaga ggacggtcct gattgcaaat acgtcagaca tgccggtggc tgctcgcgaa
     1801 tcgtcagtgt acaccgggat aactattgcc gagtacttcc gtgacatggg gtactcagtc
     1861 gccctaatgg ccgactctac atcaagatgg gctgaggcgc tccgcgagat gtctgggcgt
     1921 cttgaagaaa tgccaggtga agagggatat ccggcatacc taggctccag ggtatcggag
     1981 ttctacgaaa gagcgggcag aacaacgtgc cttggctcag atggcagaga gggtgccctc
     2041 acagtggttg gcgcagtatc acctccagga ggagacctgt cggaaccggt aacccagacg
     2101 actttgaggg ttgtgaaggt tttctgggcc ctcgatgcga acctggccta tagccgccac
     2161 tttccggcga tcaactggct cacaagctac tcgctctatc tggacaggat tgacgagagc
     2221 cttaggaaaa tggtgggcag cgagtttgtc gagatgcgcc aggaggccat gaagatactt
     2281 caggaagagg cgagtctttc ggagatcgtg aggctggtgg gcatggacgc gttgtcgccc
     2341 ggagaacgca taaccatgga gaccgcgcga agcatccgcg aggacttcct gcaccagaac
     2401 gctttccacg atgtggacac ctacacgtcc ctgaagaaac aggcggcgat gctaagggtg
     2461 atactggaga tggatcacgc gtgccgtgat gcggtcgaaa agggcgcttc gtttggccaa
     2521 ttggcggagc tggcagtgca tggaaagatc gcgagaatga agtacattga ggaagctaag
     2581 atctcggaaa ttgacgagtt ggctctcgaa gtcagacgag acgtagctgc tttgaccgcc
     2641 ggcggaggtg aggacgttgc ttaaggaata ccttactgtc agagaagtcg ccgggcctct
     2701 tatgctcgtt gaaaaggttg atgaagctaa atacggcgag ctggttgaga tagtagttgg
     2761 gggcggcgag atcaggcgcg gacgcgtgct cgaggttgaa ggaaccaacg ctcttgtgca
     2821 gctttttgag ggttcagccg gtctggagct tacgtcggcc cgggtccgat ttctggggcg
     2881 aggtctcgaa ctaggcgtgt cccctgagat gcttggacgc gtcttcgatg gattgggacg
     2941 gccaatggat gatgggccca agatcatacc agaggccagg ctcgacacga acggcagccc
     3001 tataaacccc tatgccaggg attatccatc tgagttcatc cagacaggaa tatctgccat
     3061 cgacggcttg aacacgctcg tgcgcgggca gaagctgccc atattctccg gcgcgggctt
     3121 gccccactcg cagctggctg cgcagattgc aaggcaagct agagtgttag ggacagggga
     3181 acagttcgcc gtcgttttcg gggccatggg catcaccttt gaagaggccg atttcttcat
     3241 ctctgacttc aggcgtacgg gtgccattga gcgtgccgtt cttttcataa acctagcaga
     3301 cgatcctgca atcgaacgga tttcgacgcc caagatggcg ctgacagctg ctgaatatct
     3361 cgcgtacgag cttgacatgc acgtgttggt catcctgact gacatgacca actactgtga
     3421 ggcattgcgg gaagtgtcgg cagcccgcaa ggaggtcccc gggaggcgtg ggtatcctgg
     3481 gtatctttac acagacctag ccacccttta tgagcgtgca ggcaggataa aagggaagaa
     3541 gggatcgatt acgcagatcc cgattttaac catgcctgaa gatgacaaga ctcaccccat
     3601 tccggacctg acaggctaca tcaccgaagg tcagataata gtcagcaggg agctccatag
     3661 gaaggggatc tatccgccga ttgacgtatt gccttcacta tcgaggttga aggacaaagg
     3721 aatcggagag ggcaagacaa gagaggatca cgccgacacc atgaaccagc tttatgcggc
     3781 gtatgccagg ggcaaggagg ccaaggagct agcagtcatc ctgggcgaag cagctttgtc
     3841 tccctctgac aagattttct cgaagtttgc tgatcgcttc gagcaggagt atgtgaggca
     3901 gggtgtcgat gaaaaccgga gcattgagga gacgctcgat ataggctggg atctgctggc
     3961 gatgttgcca gtggatgagc tgagccgcat caggatggag ttcatcgaga agtatctgcc
     4021 gaaagaagcc gaaaaagacg cagagcccgc gagggagtga tcggggctat ggagatcaga
     4081 gtcagcccaa caaggatgca gctgctccag ctcaaaaaga gccatgccat ggctgttcgt
     4141 gggcacaaat tgctcaaaga taagctggat gagctgatga agcaattcct cgaactggtc
     4201 aagcgggaca gggaactgag agaggaagta gatgagaagc ttggcgaggc tttcaggggc
     4261 tttcttatag cgcggggcgc gatgtcatcc agcgcagtgg atgcggcctt gatgtatcca
     4321 aaggagcaga tatctctgaa ggcttctcgg aggcatatca tgaatatcga ggttcccgag
     4381 ttcgagtgga gtttcgaggg acaagaggac aggacgaaca tctatcccta tgggttcgcc
     4441 tacacctctc cagagctcga tacggctatt aggcttcttt ccgaggccat gccgagcctg
     4501 gtggagttgg ctgaagtgga gaagtcagta gagctcctgg cgggggagat tgagaagacc
     4561 cggagaagag tcaacgctct tgaacacgtt gtcatcccgc agcaggaggc ttcgatcaaa
     4621 tatataacaa tgaagctcga cgaggcggaa cgggccaaca ttaccaggct gatgaaggtt
     4681 aaggagatag taggcgagcg gagtgtttga tcgactgctc gctggttagc cttgccctgc
     4741 aagcgaccga caaaaagtat ctcagcctga ctgagcagct aaacgtttaa actacaggca
     4801 atgcaaactc gggaatcgtt cagagacgta attggtgatc tggtagaggt tagccaatga
     4861 cggtatgtag acgccggggc gcatgtgcgt tctggcgttt tcgttttgtt ggtgagttgt
     4921 ggcatgaggg gcagatatcg gctagccgca gaggttgcct tcctgaagga ccttagctcc
     4981 atgtagaggg aggccatcgg ttgagcgcac ctactttgcc tgttctcggc gtaatagaaa
     5041 tacagagaac gacggcaaaa catgcttaca acgaggacgg gaatgaacca gcgcccaggt
     5101 ttggaaagcc tgtaaccgat cacgtagcag acattgtaga tatctggctc cttgaggatc
     5161 agttgctgtc gagaaaggaa cggggaaata ctagatgacg attgttcttg tatctcgagt
     5221 ttgacgtcgc gcgaaacgta agtaccctct tatcaggggg cttgactgtc aatacggcga
     5281 gacctttgga ctacacgctt gagttggcgc ttgttgccgg aggatctgag tcgatgtagt
     5341 tctggctctg cagtatcggg ttgatctggg ctgggctaac aacgccaaga cattgagaat
     5401 agccatctgt ttcttcgttg gcttggtcag agtcctcaca ggctgtgcta gttcgattca
     5461 tccatggtgg atgtcgccag cttcttcacc tccgacttga tggctctgaa ggcctcctcc
     5521 agcatgtttt tcttccgccg agagcttcga ttgcggcaca tttgtgcaca aacatagagc
     5581 ccatgacaag cttttagggc gtggaaccag tcgtacccta aaggctttta tctgcgaggg
     5641 cttgtcgccc tcgcctatat tgcttgtaat gagcatctca tcaaaggcaa ggccgaatat
     5701 ctgcgcgagc ttgaggcggg aaagcgtttt tattaccttt gtcatgcctc tgcgtcacac
     5761 agagctcagg gttgaacacg tcgctcgcgc tcagcggcta tggttttgta gaacgatctg
     5821 cattgagcat gcaggg
//
LOCUS       DAOSAI010000116         5816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8747_length_5816_cov_2.897761, whole genome shotgun sequence.
ACCESSION   DAOSAI010000116 DAOSAI010000000
VERSION     DAOSAI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8747_length_5816_cov_2.897761"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLM83_05200"
     CDS             <1..342
                     /locus_tag="PLM83_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOK70838.1"
                     /translation="EVSPCPGQARHDIVLAVRLGSADVVSSFCQAVQSASPVDAAVRP
                     IAAPMPGYADEVIMAAGAFVQGSSIELSADAPLRPPYDAYLQGGLIRHQVEFAMLKFA
                     QDVKEKARSRE"
     gene            397..1629
                     /locus_tag="PLM83_05205"
     CDS             397..1629
                     /locus_tag="PLM83_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70839.1"
                     /translation="MLPAKKSRTSLCAIAFLCIMAVSLAAPHGADAVWAAETVRSLLE
                     LPSADRALGNNDGATLDCLVIQESGLAVATHVVEVDLVAGMNKVRVAGVSDTIQCGAA
                     EIISGTVPFTVVSAATNRAAGELIFSVKAETASHAVFRVAYPLSGLTWSASYNAVLEP
                     TKDAAGIWGWYSIHNETDCDLAPARIVLMGGSTNPLGSKPGFKGDSALGKTVATIDSP
                     EMIPAGSQRNIRFAGGAAVPARLAYVVDWGPMAILDSSSQGKGERPVMLGLEFSASKE
                     TGLAYPLPGGRISVFSLSSDGTTFFLGEDLVSPCQSGTTLIAKVGPAVLLTAERTRTD
                     QRKVGTSSWEEAYQVRIANAGTASVTVINVEEFPGQWTILQSNIPTPPIVSGRAQFSV
                     NVPAGGQANILFRVRYTL"
     gene            1644..2495
                     /locus_tag="PLM83_05210"
     CDS             1644..2495
                     /locus_tag="PLM83_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOK70840.1"
                     /translation="MHSFPRGAVVSNSSGTCIAIEKDMASQFVGVIPEPEEAVSRIAR
                     NLQLVYGIGPVREAGLKEEGYATLFDLLSHEKWGQGARPIVEALERADGWELLRYGVS
                     GLDILAFCAPEEVVFLDIETTGLSSTSALFMVGMIIPSERGLILHQLFAREYEEELAV
                     IEETLARLADARAVVTYNGKSFDMPFLLRRLAYYGREDDPHAAIVDLCPPARRKYSFS
                     LPNCRLSAIQTMVLDVPRDGDIPGSLIPGLYNDWVRTGDAEKIIPVLDHNELDLVAMV
                     DLLPLLI"
     gene            2620..4098
                     /gene="spoIVA"
                     /locus_tag="PLM83_05215"
     CDS             2620..4098
                     /gene="spoIVA"
                     /locus_tag="PLM83_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HOK70841.1"
                     /translation="MEKFDIFRHIAERTGGDVYIGVVGPVRTGKSTFISRFMDLIVLP
                     NMKDAYEKERTRDELPQSGAGKTIMTTEPKFVPGEAVEITLGENIKVAVRMVDCVGYT
                     VPGALGYEEADAPRMVRTPWYEEEIPFQRAAEIGTEKVIQEHSTIGLVVITDGSITEI
                     GREAYIDAERRVIAELKELGKPFIVVLNSTHPEAESTRTLGEALARSYDVPVIPVDCL
                     NMSTADIMQVLGGVLYEFPIREISVKMPEWVVELEDSHPVRTGFVSIVHEAVSAIDKV
                     RDVSEAVRILGTAKNAESATVTLLDMGTGRVWIEVAAPKSLFYAVISEMSGLEVAGDS
                     DVIRMMRDLTHAKREYDKVAVALEQVRTTGYGIVAPFLDEIYFEEPDIIRQGNRCGVR
                     LKAMAPSIHMIRADIRTEVTPLMGTEKQSEEFAEQIAEDFAENPSKIWETEFLGRSMQ
                     DLIREGIEAKLAHMPIHAQEKLQETLQKIVNEGSGGLICIVL"
     gene            4262..4534
                     /locus_tag="PLM83_05220"
     CDS             4262..4534
                     /locus_tag="PLM83_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOK70842.1"
                     /translation="MTKSDLVDSVAVKTGMTKKDTARTVDAILETIKEQLKAGEKVQL
                     VGFGSFEVKKREARVGRNPKTMEEIRIPARNVPVFRAGKELKESVL"
     gene            complement(4652..5422)
                     /locus_tag="PLM83_05225"
     CDS             complement(4652..5422)
                     /locus_tag="PLM83_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70843.1"
                     /translation="MKLFSANKSFLGRYDRMLAAYAVTLLVMMCGLVGFSAATGAAGN
                     GVDEAQMVLDNMLRAQENLTSFELPATARVYLGGRNVLTANVQVLASRPNNLAVKWLG
                     VTIRPRKGIIFIDPAQFVSENYTLSVLARPSTARQPSVTPRFEDGHSNDGWVISAISR
                     PGTASPLRWKLYVDPETWLIVRAEVITADSDTCIIKAKYRNAGYRRWEPLFVHAEGKM
                     VLEEFLPDVLVGLIMGQAAREKSAYVDLDFSDGRSKKA"
BASE COUNT         1305 a         1476 c         1756 g         1279 t
ORIGIN      
        1 gaggtttcac cctgtccggg ccaggcacgt cacgacatag tacttgccgt aaggcttgga
       61 tcggcagacg tggtctcctc attctgccaa gcagtccaat cggcgtctcc ggttgatgca
      121 gctgttcgtc ccatagcagc gccgatgccg ggatacgcag atgaagtcat aatggccgca
      181 ggcgcttttg tccagggatc gtcaattgaa ctttccgcag atgcgccgct tcggccgcct
      241 tacgacgcat acttgcaggg cggactcatc aggcatcagg tcgaatttgc catgctcaag
      301 ttcgcgcagg atgtgaagga gaaagcgaga agtagggagt agacgataat agcctgtgca
      361 cgtgttgcca attgagtatt ggaggttgat tgtcccatgt tgcctgcgaa aaagtcgcgc
      421 acgagccttt gcgctatcgc ctttctttgc attatggcgg tttcactggc agcgccgcat
      481 ggcgcagatg ccgtttgggc agccgagacg gtacgcagcc tacttgagct cccaagtgcc
      541 gatcgggctc tggggaataa cgatggtgca accctcgatt gccttgtaat acaagaatct
      601 ggtcttgcag tggctacgca tgttgttgaa gtggatctcg ttgcgggcat gaacaaggta
      661 agagttgctg gcgtttccga cacaatacag tgcggcgctg ccgagatcat ttcgggtacc
      721 gtcccattta ctgtagtatc tgcggcaaca aacagggctg ccggggagct catcttcagt
      781 gtgaaggctg aaaccgcgtc ccatgcagtc ttcagggtgg cctacccgct gtcagggctc
      841 acatggagcg catcctacaa cgcggtgctg gagcctacta aggacgctgc tgggatctgg
      901 gggtggtatt cgatacataa cgagacagac tgtgatctcg caccggctag gatagtcttg
      961 atgggtggat ccactaatcc tctcgggtca aagccgggct tcaaggggga ttctgccctg
     1021 gggaagacgg ttgcgacaat agattctcct gagatgattc cagcgggttc gcagcggaat
     1081 atcaggttcg ccggcggagc ggcagtgccc gcgcgcctcg cttatgtagt tgattgggga
     1141 ccgatggcga tactcgattc ttcgtcacaa ggaaagggcg agcgccccgt catgctcggc
     1201 ttggagtttt cggcgtccaa ggagaccggg ttggcgtacc ctcttcccgg gggacgcata
     1261 tccgtcttca gcctatcttc ggatgggacg acatttttcc tcggcgaaga ccttgtttcc
     1321 ccgtgccaga gcggaacaac actcatcgcg aaggtggggc ctgctgtttt gcttactgcg
     1381 gaacggactc gcacagatca gcgcaaggtg ggcacgagca gttgggagga ggcctaccag
     1441 gtgaggatag cgaacgccgg aacagcgagt gtcaccgtca tcaacgtgga ggagtttccg
     1501 gggcagtgga ctattcttca aagcaatatt ccaactcctc cgatcgtctc ggggcgggcg
     1561 cagttcagtg tcaatgttcc cgcaggcggg caggcaaata ttcttttcag ggttagatac
     1621 acactttgag gagtggttcg gccttgcact cttttccccg tggcgccgtg gtaagtaatt
     1681 cgtcaggcac atgcattgca atagagaaag acatggctag ccagttcgtg ggggtcattc
     1741 ccgagcccga ggaggctgtc tcccgcattg cccggaatct gcagctggtt tacgggatcg
     1801 gtccggtgag ggaggctggg cttaaggaag agggctatgc gacccttttc gatctgcttt
     1861 cccatgaaaa atggggccag ggagcccgtc ctatcgtgga ggctctcgag cgcgctgatg
     1921 gatgggagct tttgcgctac ggggtgtctg gtctggatat tttggccttt tgcgcccccg
     1981 aagaagttgt tttcctcgac atcgaaacaa ccgggctttc cagtacttcg gcactgttca
     2041 tggtgggcat gatcattccg tcggaacgag gcctcatcct gcaccaactc ttcgccagag
     2101 aatatgaaga ggagcttgca gttatcgagg agacgctcgc ccggctcgcc gacgcgcggg
     2161 cggttgtaac ctataatggc aagagcttcg atatgccgtt tcttctgagg cgcctggctt
     2221 actatggacg ggaagatgac ccgcatgctg ccatcgtcga tctttgcccc ccggccagac
     2281 gcaaatactc cttttccttg cccaattgtc gactctccgc aatacagacc atggtcctcg
     2341 atgtcccaag ggacggagac atccccggtt cgctcattcc cgggctctac aatgactggg
     2401 tcaggacggg agatgcggag aagatcatcc ccgtgctcga ccacaatgaa ctcgatcttg
     2461 ttgccatggt tgatctcctt cctttgctca tctgaatccg gcataattcc ccccaagtct
     2521 catatagatg tagcgtctga gagcaggtac aacccacgtg catcctgcag gggtgtgagg
     2581 ccgcctccac ccggactcga tgcgagggag ggcgaatgga tggagaagtt cgatatcttc
     2641 cggcacattg cggaacgcac ggggggagac gtgtacatag gtgtcgtcgg tccggtgagg
     2701 acgggcaaat ctaccttcat atcgaggttc atggacctca tcgttctgcc gaacatgaag
     2761 gacgcctacg aaaaggagcg gacccgcgat gagctgcccc agagcggcgc cggaaagacc
     2821 atcatgacga ctgagcccaa gttcgtcccg ggcgaggcgg tagagatcac gctgggggag
     2881 aacatcaaag tggctgtcag aatggtggac tgcgtaggtt acacggttcc tggggcgctg
     2941 gggtatgaag aggctgacgc tcccagaatg gtacggaccc cctggtacga agaggagatc
     3001 ccttttcaaa gagcagctga aattggcacg gagaaggtga ttcaggaaca ctctactatc
     3061 gggttggtgg tcataacaga cggttcgatt actgagatcg gaagggaagc atacatcgat
     3121 gctgagagaa gagttatagc ggaactgaag gagctgggaa agccgtttat agttgtcttg
     3181 aactctacgc atcctgaagc ggaatccaca cgtacccttg gagaggcttt ggcgagaagt
     3241 tacgacgtgc cggtcatacc cgttgattgt ctcaacatgt ctacggccga catcatgcag
     3301 gtgctcggcg gcgtgctgta tgagtttccg attcgcgaga tcagtgtaaa gatgcctgaa
     3361 tgggtggtgg agctggagga ttcgcaccct gttcgcacgg gattcgtctc catagtacat
     3421 gaggcggtca gcgcaattga taaagtgcga gatgtttctg aggcagtgcg gattctcgga
     3481 acggctaaga atgccgagtc agcgacggtg acccttcttg acatgggcac cggtagggta
     3541 tggatcgaag ttgctgcccc caagtcgctt ttctacgctg tgatatccga gatgagcgga
     3601 ctggaggtgg ccggagattc cgacgtcatc aggatgatgc gcgacttgac acatgcaaag
     3661 cgagaatacg ataaggttgc tgtggcgctt gaacaggtca gaacgacagg atacggtata
     3721 gttgcgccat ttctggacga gatctacttt gaagagcccg acataatcag gcagggtaat
     3781 cggtgtgggg tcaggcttaa ggcgatggcg ccctcgatcc atatgataag ggccgacata
     3841 aggacggaag tcaccccgct catggggact gaaaaacaaa gcgaggaatt tgcggagcag
     3901 atagccgaag atttcgcgga gaacccgtcg aagatatggg agaccgagtt cctcggaagg
     3961 tcgatgcaag acttgatacg cgaaggcatc gaggccaaac tggcacacat gcccattcac
     4021 gctcaggaga aacttcaaga gacattacag aagatcgtca acgaaggcag cggaggtctg
     4081 atctgcatag tactctagct gccagctgct ttagctacgt ctggagccgt agatggcacg
     4141 gcggtgtatc tgaccgcatc gcccggtcct gtttgatatg gccattttcg aaagcccttg
     4201 aggcggaagt aataaaggct tacgtcgcgg gaaaacatac tgatggaagg gggctgagga
     4261 catgaccaag tcagatcttg tggattcggt tgccgtcaag acaggaatga caaagaagga
     4321 cactgcaaga acagtagacg cgatccttga aaccataaag gagcagctga aggcgggaga
     4381 gaaggtgcaa ctcgtcggct tcggcagctt cgaggtgaag aagagagagg caagggtagg
     4441 aaggaatccc aagaccatgg aggagatacg aattccggcc aggaatgtcc cggtgtttag
     4501 agcgggaaaa gagttgaaag agtcggttct ctaagcaatg ccccgatgtc gccagaaaga
     4561 aagggcgggg cccggtcggt gactgactcg ggctctgtgg ggtgtgcgcg acctgctgtg
     4621 atcgtgtttc aagcgtagct tgcaatgaca gctacgcttt tttcgatcgt ccatcagaga
     4681 aatcgaggtc aacataggcc gacttctccc gggccgcctg tcccatgatc aagcctacaa
     4741 ggacgtccgg aaggaactcc tccagaacca tcttcccttc agcatgaacg aagagcggtt
     4801 cccatctgcg gtatccggcg tttctgtact tcgctttgat gatgcaggtg tcagaatccg
     4861 cagtgatcac ctcagctcgc acaatcagcc atgtctccgg atcgacatag agcttccagc
     4921 gaagggggga ggcggttccg ggacgggaga tggccgatat cacccaccca tcgttgctat
     4981 gcccatcttc aaagcgcggc gtaaccgatg gctgtcttgc ggtgctcggt cgggctaaga
     5041 cagaaagcgt gtaattctca gacacgaatt gagctgggtc gatgaatatt atgcccttcc
     5101 tgggacgaat ggtcacgccg agccatttga ctgcaaggtt gttggggcgg gaggctaaca
     5161 cttgtacatt ggcggtcagg acatttcgtc cgcccagata cactcgggca gttgcaggga
     5221 gctcgaagct ggtcaggttc tcttgagcgc gcaacatgtt gtcgagaacc atctgtgcct
     5281 cgtcaactcc atttccggcg gcgcctgtgg cggccgagaa cccaacaagt ccgcacatca
     5341 taacaagcag tgtgaccgcg tacgcagcta acatgcgatc atacctgcct agaaagctct
     5401 tgttcgcact gaaaagcttc atttatcgcc acaaccttaa atcattgtct attatgtgcc
     5461 atgtggcacc tgaccaagcg atcaccttga ggttcagtgc tagctcccga ggccgtatct
     5521 gagtgagatg cgccaaaggc gctagcggag ccgaaagata gcgacagcaa ttctggtgac
     5581 gattttttgc agaccggggc ggcctgttcg catcttggcc agaattgaca cgcgtttgca
     5641 atgtgtgcct ggctgctatc gatcatgctc tcggcttcaa tagatgtagc gaaagacgcc
     5701 tgcgccggac tgtgcccccg gccgtttcgg ttccggcggc gacgcccccc agttcccggt
     5761 attgccccaa gtagctgacg tgtaaacgga tggagcggcc tttcgtgtag agcgtc
//
LOCUS       DAOSAI010000117         5800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8778_length_5800_cov_3.888947, whole genome shotgun sequence.
ACCESSION   DAOSAI010000117 DAOSAI010000000
VERSION     DAOSAI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8778_length_5800_cov_3.888947"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1141)
                     /locus_tag="PLM83_05230"
     CDS             complement(2..1141)
                     /locus_tag="PLM83_05230"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009792728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK70844.1"
                     /translation="MEFRDLKAQYLALQEKIDAKIKEVLCEGRFILGRQVFELEEKLA
                     EYVGVKHCITCANGTDALLLVLMAWGIGPGDAAFTSDFTFIASASVASLRGATPVLVD
                     IDPKTYNISPDALEEAIERTFREGKLTPRVIIPVDLFGLPADYPRIEKIAQKYNLLVL
                     EDGAQGFGGSINGRKACSFGNAATTSFFPAKPLGCYGDGGAIFTDDDELAATLRSIRA
                     HGRSPEDKYDNQTLGLNSRLDTLQAAILLTKLEAFIEYELDAVNRVADVYTENLSDIV
                     QTPVVPEGYYSSWAQYTIRLDDKEERDTLQRKLAEQGIPSMVYYPRPLHSQTVFSFLG
                     LDSGAYPNSARAAETVFSVPIHPYLSKADIERGAGEVKDFIAAFG"
     gene            complement(1143..1727)
                     /locus_tag="PLM83_05235"
     CDS             complement(1143..1727)
                     /locus_tag="PLM83_05235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015595955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOK70845.1"
                     /translation="MDYFAHESSYIDDGVKIGRGTKIWHFCHIQSGAQIGEFCTLGQN
                     VNVGTNVKIGNYVKIQNNVSVYEGVELEDYVFCGPSMVFTNVLTPRAKYPVADKELYA
                     KTLVKEGATIGANATIVCGNTIGRYALIGAGAVVTKDVPDYAVMVGVPARQVGWACEC
                     GQILDDGLECEKCGRKYAATGTATGTAIALLEEE"
     gene            complement(1733..2815)
                     /locus_tag="PLM83_05240"
     CDS             complement(1733..2815)
                     /locus_tag="PLM83_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK70846.1"
                     /translation="MRYALIGCGRISPNHLAAVQAHSGDIELVAVCDLVPERMDVALA
                     EAQINGGKVKKYTDYQAMLSSEQLDLVAIATESGSHAAIGLDCINAGCHVIIEKPMAL
                     SIVDANRLIDAAKAQGVVLCACHQNRFNKSIQKLRKAIEQNRFGRLFHGAAHVRWNRN
                     RGYYEQAPWRGTWAQDGGALMNQCLHNIDLLRWMMGDHIDEVFAYTARLNHEYIEAED
                     LGLALIRFSNGAYGLIEGTTNVYPKNLEETLYIFGEQGTVKVGGKSVNIIEEWSFADQ
                     KDDPEEIKAKYHENPPNVYGFGHTPLYRDVVLAIREGRRPLVDGEAGKRAVELILAIY
                     KSALEGRPVRLPLEDFSTRDMKGFFG"
     gene            complement(2825..4132)
                     /locus_tag="PLM83_05245"
     CDS             complement(2825..4132)
                     /locus_tag="PLM83_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOK70847.1"
                     /translation="MTTLEQRLRDKSALLGVIGLGYVGLPLAVEKAKAGYRVIGFDIQ
                     PHKVDMVNQAINYIGDVVDEDLATIVEAGMLTATNDFSRISELDCVSICVPTPLDRYQ
                     QPDLSYVKSTAETIAKYMHRDMLIVLESTTYPGTTEELLKPILESSGLKCGTDFFLAF
                     SPERVDPGNRIYKTKNTPKVVGGVTPTCTELAATLYESILQAEVFRASSPAVAEMEKI
                     LENTYRNINIGLINELAMLCDKMGINIWEVIEAAKTKPYGFQAFYPGPGLGGHCIPLD
                     PYYLSWKAREYGFHTSMIEASMMINDRMPEYCVERAARILNKVKKPLNGSKVLVLGVA
                     YKQDIDDYRESPAIKVINHLEERGAEVVFFDPYIKEYKHEGKVKRGADALTKELIESA
                     DLVMITTAHTTVDYDSVQQHAKLVFDTKNAMKKVRDRSNIEVL"
     gene            complement(5174..5551)
                     /locus_tag="PLM83_05250"
     CDS             complement(5174..5551)
                     /locus_tag="PLM83_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70848.1"
                     /translation="MPYRVRHKDKYSNYWFSSSDGHTIEEFNALTDPKNVLKLEEQGG
                     LCIVYTHFASGFVKDSGKVDPIFERNIRFLASRDGWFAPAGEILDYLLSREVVEFASR
                     SYLARLDAVWLLQRLVKKLRYGR"
BASE COUNT         1443 a         1629 c         1248 g         1480 t
ORIGIN      
        1 cctaaccaaa cgctgcaatg aaatccttca cttcgcctgc gccgcgttct atgtccgcct
       61 tagatagata cggatgtata ggcactgaga ataccgtctc cgctgctcta gcagaattag
      121 gatacgcccc cgagtccagt cccaagaaag aaaagacagt ctgagaatgc agaggtcgag
      181 ggtaatacac catcgatgga attccctgtt ctgcgagctt tcgctgcaga gtatcccgtt
      241 cttccttgtc atccagcctg atcgtatact gagcccaact cgagtagtat ccctctggca
      301 ccaccggcgt ttgaactata tcactcagat tctcggtgta gacgtctgcc accctgttta
      361 cggcatcgag ctcatactca ataaaagctt ctagcttagt cagcaggatt gctgcctgga
      421 gggtgtcaag cctcgagttt aaccccaacg tctggttatc atacttgtcc tcaggcgaac
      481 ggccatgggc ccggatcgaa cgcagagtag cagctaactc atcgtcatcg gtaaaaatgg
      541 cgccaccatc accgtaacag cccaggggct ttgccgggaa aaaggaagta gtagccgcat
      601 taccaaatga gcaagccttt ctgccgttga tacttccacc gaaaccctga gcgccatcct
      661 ctagaaccag caagttgtat ttctgggcaa tcttctcaat tctagggtaa tcagccggca
      721 gaccaaacag atccacggga atgatcacgc ggggagtaag cttgccttca cgaaacgtcc
      781 tctcaatggc ttcctctaga gcgtcgggag atatgttgta tgtcttggga tctatgtcta
      841 ccaaaactgg agtggcccct ctcagagatg ccacacttgc tgaagcaata aaggtgaagt
      901 ccgaggtgaa agctgcatca ccaggcccaa tcccccaagc catgagaaca agtaacaaag
      961 cgtcagtccc attggcgcag gtaatacagt gcttcacacc aacatattcg gcaagcttct
     1021 cttccaactc aaaaacctga cggcctagga taaaccttcc ctcacacaat acttctttga
     1081 tcttggcgtc aatcttctcc tgtagcgcta gatattgggc cttaaggtct ctaaactcca
     1141 ccttactcct cctccaacaa agcaattgcc gtacctgtag ccgtacctgt agccgcatac
     1201 ttgcggccgc acttctcgca ctcgaggcca tcgtctagaa tttggccgca ctcacaagcc
     1261 cagccaacct ggcgagcagg tacgcccacc atcacagcgt aatcggggac atcctttgta
     1321 acgactgccc ctgcaccgat cagggcatag cgcccgatgg tgtttccgca cacgatggtg
     1381 gcattggctc caatggtagc tccttctttc acaagtgtct tggcgtaaag ctccttatcc
     1441 gcaaccgggt atttcgccct gggcgtcaag acgtttgtaa ataccatgga tggcccacag
     1501 aacacatagt cctcaagttc cacaccctcg tagacggaaa cgttgttctg gatcttgaca
     1561 tagtttccga tcttgacgtt ggtgcctaca ttaacgtttt gccccagcgt gcagaattca
     1621 ccgatctggg ccccagattg aatatggcag aaatgccaga tcttcgttcc tcgaccgatt
     1681 ttcacaccat catctatgta ggagctttca tgagcgaaat aatccatagg aatcacccaa
     1741 aaaacccttt catgtccctg gtcgagaaat cctctagagg caggcgcaca ggcctacctt
     1801 ccagtgctga cttgtagatg gctagaatca actccacagc cctttttcca gcttcgccat
     1861 ctaccagtgg cctccgcccc tcgcggatgg ccaaaaccac atccctgtac aatggggtat
     1921 gtccaaaccc ataaacattg ggcgggttct catggtattt cgcctttatt tcctcgggat
     1981 catccttctg atctgcgaaa ctccattcct caatgatatt caccgatttt cccccaacct
     2041 tgacagttcc ctgttctcca aaaatgtaca gggtttcctc aagattcttg ggataaacgt
     2101 tggttgttcc ttcgatcaga ccataggcac cattggagaa cctaatcaga gccagcccca
     2161 agtcttccgc ttcgatgtac tcgtgattta ggcgagctgt ataggcaaac acctcatcaa
     2221 tgtgatcgcc catcatccag cgcagaagat caatattgtg gaggcactgg ttcatgaggg
     2281 ctccgccgtc ctgggcccag gtaccccgcc aaggtgcctg ttcatagtat cccctattgc
     2341 gattccatct aacatgtgcg gcgccgtgga acagccttcc aaaacggttc tgttcaatag
     2401 cctttcgtaa tttctggatt gacttgttga agcgattttg atgacatgcg cagagcacca
     2461 ccccttgggc cttagcggca tctatcaacc gattcgcatc aacgatagac aaggccatag
     2521 gcttttcgat aatcacgtgg cagccagcat taatgcaatc tagaccaatg gcagcgtggc
     2581 taccgctttc cgtggcaatc gccaccaaat ctagttgctc cgaacttaac atcgcctgat
     2641 agtcggtgta tttctttacc ttgccaccgt tgatttgagc ttcggccaga gccacgtcca
     2701 ttctctcggg caccagatcg caaacggcca caagttcaat atcaccgctg tgtgcttgaa
     2761 ccgccgctag atgattgggt gagatccttc cgcagccgat aagtgcgtaa cgcagttttc
     2821 ccactcacaa cacctcgata tttgatcgat cccgaacctt tttcatagca ttcttggtat
     2881 caaagaccag cttggcatgc tgctgtacag aatcatagtc cacagtcgta tgggcggtag
     2941 taatcataac caagtccgca gactcgatga gttccttggt taaggcgtct gcgccgcgct
     3001 tgaccttacc ctcatgcttg tattccttga tatacggatc gaaaaagacc acttccgcgc
     3061 cccgttcctc aagatgattg atgaccttga tagcagggct ctcccggtaa tcgtcgatgt
     3121 cctgtttgta ggctacgcca aggactaaca ccttagaccc atttaggggc ttcttcactt
     3181 tgttcagaat tcgcgctgcc cgctccacgc agtactcagg catacgatcg ttgatcatca
     3241 tcgacgcctc tatcatagag gtgtggaagc catattccct agctttccaa gacaaatagt
     3301 aaggatccaa gggaatgcag tgtccaccca gacccgggcc gggataaaag gcctgaaacc
     3361 cgtagggttt tgtctttgca gcctctatca cttcccatat attgataccc attttgtcgc
     3421 acagcatggc cagttcattg atgaggccga tgttgatgtt tcgataagtg ttttcaagga
     3481 tcttctccat ttcggccact gctgggctgg aggctctaaa tacctcagct tgcaaaatgc
     3541 tttcatagag agtagctgct aattcggtgc aggttggggt cactccccca acaacttttg
     3601 gtgtgttttt ggtcttgtag attcgattgc ctggatctac cctttcaggg gagaacgcta
     3661 ggaagaaatc ggtaccgcac ttgagaccac tgctctccaa aatgggtttt aggagctctt
     3721 ctgtagtgcc aggataggta gtggactcca gcacgattag catatcccta tgcatgtact
     3781 tcgcaatggt ttctgcggta ctcttaacat agctgagatc gggctgctga tagcggtcca
     3841 agggggtagg cacgcagatg cttacacagt ccaattcaga gatcctgctg aagtcattag
     3901 ttgcagtaag catcccagcc tcaacgattg tcgcgagatc ttcgtcaact acatccccta
     3961 tgtagttgat agcttggtta accatgtcca ctttgtgagg ttgaatgtcg aagccgatga
     4021 ctctatatcc cgccttagcc ttttcaactg ccaggggtaa acccacatac cctaatccaa
     4081 tgactcccag caaagctgac ttgtccctca aacgttgctc aagtgtggtc atttggacac
     4141 tcctttcgcg ttgccttcaa cccatctcat ccacatccta tcgaaaaagc catacataga
     4201 aagaccgttt actacgcggg atttcatcat ttgctgctca caggactaac ggacgtgtta
     4261 atccagaatc cagcgccacg ctgtgcttct ctatgtcagt atcagaaagc ctgctatgtc
     4321 cgattcgtct ccacgtttca ggttctccag ccgaaactag ccagccgtga aaacgccaga
     4381 tgtttgacat gtcacaagag acttccaaga aagcccgcaa gtacagacat ccagagtatc
     4441 gctacgtcgt tgcatacgtc cacgtcatct ttatgtagaa ccgttcgccc acccacgagc
     4501 cacatgagat ttcttgttca cctagttggt cgactaagca cctttaggta ggtctgccac
     4561 tagtataaac caacttggac atttacctca tgaactgact tctcatactg tattgttctc
     4621 atggactgct aagacaagta catgcataat tgttcacaaa accggatcgc cctctcgttt
     4681 ttgctcccac cgataatcga taactgctat atccctcttg aaggcaattc tcctcaaaag
     4741 cacccactag cgccatggcg acccccttac ctctgaactc cggctagccg atagaatcgc
     4801 tctcctactc ggcaaccggc ggttattttc tttcacacac cgacacaggc aatctttatc
     4861 acaccaaatt gacagaaaag cgccccaatc taactatacg caattatagc ggacagatct
     4921 cctcagggct acgctagcat gatgcgtacc tatcacaaac tattaacata ccagtttcag
     4981 ctgcgcaaca cttacgaggg atagctctct gggcggtaag aaacgcctgc caatgcgcgg
     5041 ctagcaactc cttggccgat ctcgccacta atccgtgtga aaacgcatgc tggtggaggg
     5101 caactacttg actgaggtct cctctactaa ccgtcctcat catcacatcg ccccacaacc
     5161 ggtatactag aggctatcta ccgtatctca acttcttgac caaacgttgt agtagccata
     5221 cagcatccaa acgcgctaaa tagcttctgc tggcgaattc aacaacctcg cgggagagca
     5281 agtaatccag gatttcaccc gcaggagcga accatccatc cctgctagca agaaaacgga
     5341 tatttcgctc aaagattgga tctaccttac cactgtcttt cacaaaacca ctagcgaagt
     5401 gcgtgtagac tatgcacaaa ccgccttgtt cttccagctt cagtacattc ttggggtcag
     5461 tgagagcatt gaactcctct atggtgtgac cgtcagatga actgaaccag taattactat
     5521 atttgtcctt atgacgaact ctgtagggca tcctcgggtc gtatcgcagc gtgttgatcc
     5581 cgttaaaagt gtgattccta atgaatttga catgcttttt tgcaacgtct ccccaaaaat
     5641 actcggactc aggattgttc ccttaataga cccgtctatc accgaacata agcttcatca
     5701 gagacgaaac caagccggtg aagcgctcaa ccccccagta taaattgtca gggttgcttg
     5761 cgtggttgat ctgcatggta gggtaaaaac ccatcaactc
//
LOCUS       DAOSAI010000118         5796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8781_length_5796_cov_3.757708, whole genome shotgun sequence.
ACCESSION   DAOSAI010000118 DAOSAI010000000
VERSION     DAOSAI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8781_length_5796_cov_3.757708"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(807..1517)
                     /locus_tag="PLM83_05255"
     CDS             complement(807..1517)
                     /locus_tag="PLM83_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017718856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70849.1"
                     /translation="MNEDRQAQAFIQLEDVCKVYAMGDVSVAALGGISLEIPRGEYVA
                     IMGPSGSGKSTLLNIMGGLDVATSGKYLLEGVEVTRLPDAELARIRCRHFGFIFQSYN
                     LFPELTAIENVIVPMMYAGVAPANRTQRAAELLDSLGMGHRLRHLPSQLSGGEQQRVA
                     IARALANDPDVIFADEPTGNLATAQGEEILDILEGLNKRGVTIVIVTHSENVADRAER
                     LITMRDGRVYSDQTAARV"
     gene            complement(1514..2839)
                     /locus_tag="PLM83_05260"
     CDS             complement(1514..2839)
                     /locus_tag="PLM83_05260"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK70850.1"
                     /translation="MAAGTPETEREYEPIVTSPARTSSSKTRRDMGMLIFIIAVLVGA
                     VALAARFLIPKEKPYLLSNYTFAHVSTADFTDSVSAPGTVAPATTVDVRADVSGVVAK
                     LSAALGDEVNVGDPICVLQSPELVTRRDEAVRQVVAAEDALAKAKLDAAQSEERVRRE
                     IVVAEQAVSDALNRREDMEKLFEAGAVSRVQVDEARAAESKARMALDNARLDHDATIR
                     SNALSIDTATRQVGVARNALRAAEDCISQLTVCSPIKGKVLELSVVLGATASQGGVVA
                     QVADMESIVVKAKVASTDVRRVTVGQPASINLGGRKVAGVVRHVAPKAEASGQGATVE
                     VTIDFDERPGDVPPNSAAFVDIEVQRRTDVPSLPRGAYLASSRELFVYVIEGSKAVQR
                     DVRFGSIFGNRIEVVDGLSVGESVITSSYEEFKDRREIKVAPEGGRESQ"
     gene            complement(2857..4308)
                     /locus_tag="PLM83_05265"
     CDS             complement(2857..4308)
                     /locus_tag="PLM83_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70851.1"
                     /translation="MTSHLFKSAWINMSRRPMQSILTIIQVALAVACMVSVASYRMNV
                     STAVDRFKSCTDDIVIAMGGMEKRTEGGYMRNMYNIFDDDDIAQLNAEVSLVESVTVY
                     EDMWDIEADVDGTLYRIGLGALVGPGYQDVVGFDMIEGNFITDVDMKAQSRVVVISEA
                     LAKILFGEGPYVGRTLGIMPVWARRSFANEDGAMPVAPTEYHVIGVYSNSKTSTSAMS
                     SYSGMHPAILCPLTSDPNQIYSSGQGWLCMDEEYYPYGTLMIKAAPGKAAAVRDRIVA
                     MVSGRPSPDSLSGSSGSMVVMGGGSAEFDGPGSDGEGSDWYSPPDEESGASVIFERTD
                     DLEQMIGSSMTRMTLLLGGATLIAIIVSALGIQSAMMVNVTERTREIGLRRVLGASRR
                     VVVVQFTVDAMILAAVGGGFGGLLSFALYPYLMNSVFSSMGPGWLLDVSARPVGWAIL
                     AGIVLSAAVGALFGAIPAAQAARVQPADIVREL"
     gene            complement(4313..5695)
                     /locus_tag="PLM83_05270"
     CDS             complement(4313..5695)
                     /locus_tag="PLM83_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70852.1"
                     /translation="MRFADLIATAVRRCRARLLESFLIVLGIALGVAVVAAFSGLIGT
                     VGKEYRQFSDSIAFREICIESTSGRSGGSYSTDRPAFALSRTGEAPYELSLHDVDRIK
                     SRVSPDVYVYAYEDFFQHLRPGDDLSVGSSLDDSIPKSSENPEKGPVPRNVRVIAATE
                     EVFSAYGAEFREGIGFSRPDVGSGERCMVLGSRLAERFFGDAPAVGKKMIWQNEWMDG
                     PTGELELSPSGYVYTVIGVMERIEPPVSAGKAGESPDDIDFSIDGSAYIPITSAPGME
                     GPNPKVHEIHAISALSKDAARAMDEVRQAIETEYDGRLDASSGMEYMKESMRELRSFA
                     TGILVLASAGLVIASINILNLMLARVLRRTREIGISAALGAGRREIFRESLAESLVLG
                     ISGGVIGLPLSYGLVKLMSRIMEGLDVSVGLTGVLAGALSCIVVSVIFGLYPAYLAAC
                     IIPADALRGD"
BASE COUNT         1335 a         1781 c         1417 g         1263 t
ORIGIN      
        1 cgatcacgct cctgtctgct gttttgtcga cgatataatt gcgaagacaa gttggactgg
       61 gcggacacag tgcgcactaa agaaaaaatg ctccccgtcc cgcaaaggga cgagaagcat
      121 gatcttcccg tggtcccacc ctaattagcc cgaagctggc gcctcgaacc cactcatcaa
      181 ggatacggac agcggcgcaa caggcgacag aagcagcgct gccttatatc ccctttcctg
      241 taacggtgaa agactccgtc ggaacctaat agcagaacgc tttcgcgccc gcgttcagcc
      301 cgcactcccg ggtgtggttc agtcgctctt tcggaccggg ctcgcaccgt ccccggctcg
      361 ctgcccgaaa ggtccgcgcc tactcttccc gatcatcgct ttaacgtgtg tatttgtata
      421 ttacgttaga tgatagcacc tcaggggaga ttatgcaata ggaaaaagtc cctcccccca
      481 tcttgtgctt ctgcgcaact tgcgaagagc atcttgatct gagcaggcgg ccaagatctg
      541 aactaaagag aatcgcgcag tggcttatcc acacttgctg tgggactctc attgtgacaa
      601 aagcaaccgt tggatatctg caatcctctt ttcggggaga atatgtatgg ccgctggcag
      661 aatgggcgcc atttgcggat ctcaggagag actggcagtc cggccgacta aagagacgac
      721 gttacgcaga cggatttgag ctctgttttg agttgcgggt caaatgatga gaggttgtca
      781 gtcaggcaag gcttgctgcg cccgacctac acccgagccg ccgtctggtc tgaatatact
      841 cgcccatcgc gcatggttat gagtcgttct gcgcggtcgg caacgttctc gctgtgagtc
      901 actatcacta tggtaacccc tcttttgttg agcccttcaa gaatatccaa tatctcctcg
      961 ccctgcgctg tggccaggtt tccagtaggc tcgtcggcga atatcacgtc ggggtcgttg
     1021 gcaagcgccc ttgcaatcgc aacgcgctgc tgttctccgc ctgaaagctg acttgggaga
     1081 tggcgaagcc ggtggcccat ccccagcgaa tcaaggagct ctgcagcccg ttgcgtccga
     1141 ttagccggcg caacgcctgc gtacatcatg gggactatca cattttcgat tgctgtgagt
     1201 tcgggaaaga ggttgtaact ttgaaagatg aatccgaagt gacggcacct gatcctggca
     1261 agctcagcat cgggcagacg cgttacctcg acgccctcta gcaggtattt acctgatgtt
     1321 gccacgtcca aaccgcccat gatgttgagc agagtggact tccccgaacc ggacggtccc
     1381 attatcgcta catactcccc acgggggatt tcgagtgaaa ttcctcccag cgccgcaacg
     1441 ctgacatccc ccatcgcgta tactttgcac acgtcctcaa gctgtatgaa agcctgagcc
     1501 tgtctatcct cgttcattgg ctttcccgtc ctccctcagg cgcaactttt atctcccgcc
     1561 tatccttgaa ctcctcatag gatgaggtga tcacgctctc gcccaccgac agcccgtcta
     1621 ctacttcgat tcggttgccg aagattgaac cgaatctcac gtcccgttgg accgctttgg
     1681 aaccttcgat tacgtatacg aacagttctc tgctgctggc caggtaagcg ccgcggggca
     1741 aggacggcac atctgtacgt ctctgcacct ctatatcgac gaatgcagca ctgtttgggg
     1801 gcacgtcgcc cggccgttca tcaaaatcga ttgtcacttc aacagtcgct ccctgtccgc
     1861 tcgcttcggc cttgggcgcc acatggcgaa ctactccagc cacctttctg cctcccaggt
     1921 ttatcgaagc aggctgccct accgtcaccc ggcggacatc tgtggaggca actttggcct
     1981 ttaccactat gctctccata tccgccacct gggcgaccac tcccccttgg ctcgctgttg
     2041 cgcctaaaac gacactcagt tccaacacct tgcctttaat aggcgagcag actgtgagct
     2101 gtgaaataca atcctcggca gcgcgcaaag cattccgtgc cacgccaacc tgccttgtgg
     2161 ccgtgtcgat cgagagggcg ttcgacctaa tagtggcatc gtgatccaac cgcgcgttat
     2221 ccagcgccat acgcgctttg ctttccgcgg cccgcgcctc gtccacctgg actcgggaca
     2281 cagcgcccgc ctcgaacagc ttctccatat cttcacgtcg attaagtgca tctgaaaccg
     2341 cctgctcggc tacgacgatc tctctcctca cccgttcctc cgattgagca gcatcgagct
     2401 ttgccttggc gagtgcatcc tcagcagcaa ccacctggcg cactgcctcg tcacgtcttg
     2461 taacaagctc tggcgactga agaacgcata tggggtcgcc cacattcacc tcatcaccta
     2521 aagcggcgct gagcttggca accaccccag aaacgtcagc acgcacgtcc actgtggtag
     2581 ccggcgctac ggttccgggg gcgctcacag agtcagtgaa atcagccgtg ctgacatggg
     2641 caaatgtgta gttcgagagg aggtagggtt tttctttggg tatgagaaac cgtgcagcca
     2701 gggcaaccgc gccaaccaac acggctatga tgaatatgag catgcccatg tcgcggcggg
     2761 tcttgctcga tgaagtccgc gccggactgg tgactatggg ctcatactct ctttcggttt
     2821 ccggcgtacc tgcagccata agaatacctc cccgaatcaa agctctctca ctatatctgc
     2881 aggctgaaca cgggcagcct gtgccgcagg tatcgcccca aagagggcac ccactgcggc
     2941 ggaaagaaca atcccggcta atatggccca tcccacaggc ctcgcactca cgtccaaaag
     3001 ccatcccggg cccatactgg aaaagacaga attcattagg tatggataca gggcaaatga
     3061 cagaagccct ccgaatccgc ctcctactgc cgcgagtatc atggcgtcaa cggtgaactg
     3121 gaccaccaca acgcggcggg aggcgccaag aacgcgcctt aagccgatct cgcgggtgcg
     3181 ttcagtcaca tttaccatca tagcactctg aatgcccaga gcgctcacga ttatcgcaat
     3241 aagcgtagcg ccgccgagca acaacgtcat cctagtcata gatgagccta tcatctgctc
     3301 caagtcatca gttctctcga atatcaccga cgcgccgctc tcttcatcag ggggcgaata
     3361 ccaatcggag ccttcgccat ctgaccctgg cccgtcaaat tcagcgcttc cgcctcccat
     3421 gaccaccata ctcccactac ttccgctcaa tgaatcggga ctcggacggc ctgacaccat
     3481 ggcaacgata cggtcacgaa ctgctgcagc tttgccgggg gcggccttga tcatcaatgt
     3541 gccgtatgga taatactctt cgtccatgca taaccagccc tggcctgaac tgtaaatctg
     3601 attgggatcg gacgtgagcg gacagaggat ggctggatgc atgccactat agctcgacat
     3661 cgctgacgta gacgtcttag aattgctgta tacgcctata acatggtatt cagtgggtgc
     3721 cacgggcatg gccccgtctt cattagcaaa actccgtcgc gcccagactg gcataatgcc
     3781 aagcgttctg cctacgtagg ggccctcccc aaacagaatc ttcgcgaggg cttccgagat
     3841 aacgaccacg cgggactgcg ccttcatatc cacatcggtt atgaagttgc cttcgatcat
     3901 gtcgaagccg accacgtctt gatagccagg cccgactaac gcacctaagc caattcgata
     3961 caacgttcca tccacatcag cctcaatgtc ccacatatcc tcgtaaacag ttacagactc
     4021 cacgagggaa acttcagcat tgagttgggc aatgtcgtcg tcatcgaaaa tattgtacat
     4081 gtttctcata tacccgccct cagtgcgctt ttccatcccg cccatggcaa tcactatgtc
     4141 atcagtacaa gacttgaaac ggtcgaccgc tgtcgacaca ttcatcctgt agcttgccac
     4201 cgaaaccatg caggctactg caagagccac ttgaatgatc gtcagaatag actgcattgg
     4261 gcgtcgactc atattgatcc acgcggattt gaacagatgc gaagtcatcg gcctaatccc
     4321 ccctcagggc atccgccggg attatacagg cggcgaggta ggctggatag agtccgaaaa
     4381 tcacgcttac tacgatgcag gacaaagccc cggctaaaac ccctgtaagg ccgacggaca
     4441 catcgagccc ctccataatg cgggacatca gctttaccag gccgtaagag agcggaaggc
     4501 ctattacgcc tcctgatatc ccaagcacca gcgactccgc cagactctct cggaatatct
     4561 cacgccggcc tgcgcccagg gcagccgaaa taccgatctc ccgagtacgc cgcaaaaccc
     4621 gtgcaagcat taggttgaga atgttgatcg atgctatgac caatcccgcc gaagccaaaa
     4681 ccagaatgcc agtggcaaag cttcgcagct cgcgcatact ctctttcatg tattccatcc
     4741 ccgagctcgc gtcaagacgc ccatcgtatt ccgtctctat cgcctgcctc acctcatcca
     4801 tagctcgtgc agcatctttg gataaggctg atatagcatg gatctcgtgg actttcggat
     4861 tggggccctc catgccaggg gctgaagtta tgggaatata ggcacttcca tcgatggaga
     4921 aatcaatgtc atctggagat tcgcctgcct tcccagctga aaccgggggc tcaatccgtt
     4981 ccataactcc gatcacagta tagacatacc cggaaggcga aagctcaagt tcacctgtgg
     5041 ggccatccat ccattcgttc tgccatatca tcttcttccc tacagcaggt gcatctccaa
     5101 agaacctctc agccagcctg ctgccgagaa ccatgcacct ttcgccggaa cctacatcag
     5161 ggcgggagaa ccctatgcct tcccggaatt cggcgccgta ggcggaaaac acctcttccg
     5221 tggcagcaat cactcgaaca tttcgcggca cagggccctt ttccgggttt tctgaagatt
     5281 tcggaataga atcgtccagg gatgaaccga ccgagagatc gtccccaggc cgcagatgtt
     5341 gaaaaaagtc ctcatatgcg tagacgtaca cgtcaggaga aaccctagac ttgatgcgat
     5401 ccacatcatg tagagatagt tcgtacggcg cctcgccagt ccgcgacagg gcaaacgccg
     5461 gcctatcagt ggagtagctt ccaccgcttc gccctgacgt tgactcgata cagatctctc
     5521 tgaatgctat ggaatcactg aactggcggt actcttttcc tactgtgccg atgagtcctg
     5581 aaaaagcagc caccactgca actccgagag caatgcccag cacaatgagg aacgactcca
     5641 ggagccttgc gcgacaccgc cgcacagctg tggcaataag atcagcgaac ctcatacggc
     5701 cacctcccga tgtgtaaatg aggaaagact catctcaact aatcatagca gaaatacagc
     5761 ttgtaaccta attgccataa tacccgcaac ttggcc
//
LOCUS       DAOSAI010000119         5790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8792_length_5790_cov_3.545597, whole genome shotgun sequence.
ACCESSION   DAOSAI010000119 DAOSAI010000000
VERSION     DAOSAI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8792_length_5790_cov_3.545597"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PLM83_05275"
     CDS             <1..902
                     /locus_tag="PLM83_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70853.1"
                     /translation="SIMGLLPSPGRIVGGRVMFGGTDLVDLSEAEMRTIRGKEIAIIF
                     QDPMTALNPAYRVGEQIAESLRVHGMVPVSAGDTGRLCGDGGGLGPRMRKTRSRKLSA
                     AEEREYVFELMQSVGIPMPEQRHLDYPHQFSGGMQQRIMIAIALACKPKLLLADEPTT
                     ALDVTIQAQVLDLIDEINETTGTAVVLVTHDLAVAADFCDSIAVMYAGQIVERGPTEA
                     VMEGSAHPYTRGLLRSMHVLGERKPLRPIMGEVPDLANLGPGCSFAPRCDFSEDVCFE
                     RAPDVSIVEKGHEVRCHHHSGAL"
     gene            complement(1121..3277)
                     /gene="vanT"
                     /locus_tag="PLM83_05280"
     CDS             complement(1121..3277)
                     /gene="vanT"
                     /locus_tag="PLM83_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HOK70854.1"
                     /translation="MNLQEEFPGIDYFRIIAAFLVVANHTSPLSSVNGTADFILTRII
                     ARVAVPFFFMTSGFFLFRGKATDSEADRARLFRFLRKTAALYAISTLLYLPVNIYAGT
                     FRTEHLAKELLKDIVFDGTFYHLWYLPAAMIGATIAWLLLKRLGPGHAFGIAFILYLV
                     GLFGDSYYGIAERVPFLQSFYASVFTVSDYTRNGLFFAPVFLVLGAMMAPPARRLQPK
                     ACFTGLAVAFLLMLAEALLLHTCGIPRHDSMYLTLLPCAALLFQSLLHINGKTRKELR
                     DISTLIYLVHPMMIILVRWLAKAAGLKRWLIANSVIHFLAVAVSSLAAAEAILLLLRV
                     FQNRELAPEHATMDRAWAEIDLTCLSHNVQVLRKAMPDGCKLMAAVKGNAYGHGDVEV
                     AKHLNQIGIDAFAVAAIDEGIRLRKHGIRGTILILGYTVPERAGELARYRLSQTVVDR
                     EHAVRLNACGKPIHVHIKVDTGMHRMGEPWDHVSEIASIFGLKNLCIDGIYTHLSVSD
                     SMEPEDAQFTEMQIDRFYHLLETLREQNVTLPKIHVQSSYGLLNYPMLKCDYARVGIA
                     LYGTLSSPHDQTRLHLDIKNVLSLKARVSLVRTVTAGESVGYGRHFIAERDTCVAILP
                     IGYGDGIPRNLSGGRGSVLIHGCRAPIIGRICMDQLMVDVTGIPDVKRGDVATLIGRD
                     GSAEITAEQAAADAGTITNELLSRIGIRVKRVFYSS"
     gene            complement(3274..4038)
                     /locus_tag="PLM83_05285"
     CDS             complement(3274..4038)
                     /locus_tag="PLM83_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOK70855.1"
                     /translation="MRTLKLYAEDIGKGNLILVNPSHPLATTPADEVLVPIRPAFPEI
                     VLERQTARMLAEVAALLQCEDQIVPVSGYRTMQEQQAIYADSMRENGKEFTKKYVATV
                     GCSEHQTGLAIDLGENRPDIDFIRPYFPYTGICQEFRKLSVQYGFIQRYPAGRESITQ
                     IAHEPWHFRYVGYPHSQLIRGNEFTLEEYTDCLKHFPFDGAHLRIKHDNYDFEIFHVP
                     VEPGQEVEIELPDRIPFQVSGNNDDGFVVTLWRHLG"
     gene            complement(4035..5084)
                     /gene="vanG"
                     /locus_tag="PLM83_05290"
     CDS             complement(4035..5084)
                     /gene="vanG"
                     /locus_tag="PLM83_05290"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HOK70856.1"
                     /translation="MKRTRVAVIFGGSSTEYEISLQSAYSVITNLNPEEYDLILLGIT
                     RQGAWMRFTGPVEQIKDNTWMDHSVSVPAIISPDRNTHGILEFHGNKVTTTRIDVAFP
                     VLHGKNGEDGTVQGLLEMAGIPCVGCGVLSSAMCMDKDIAHRIVSLAGVKTPPSIVLH
                     SYVSEAELLKRIAHLKYPLFVKPANAGSSFGITKVSQESGLACAVSAAFEHDRKVVIE
                     ESIYGFEVGCAVLGNDVLTIGEVDEIELCNGFFDYTEKYTLKTSKIHMPARIDVETAA
                     RIKQTAIVIYRALECSGYARVDMFLTPEEDIVFNEVNTIPGFTTHSRYPNMLKGIGMS
                     FEQIVDTLIRLAIDR"
     gene            complement(5587..>5790)
                     /locus_tag="PLM83_05295"
     CDS             complement(5587..>5790)
                     /locus_tag="PLM83_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK70857.1"
                     /translation="PSAKLETIFDQFFRLDASRSTNSGGAGLGLAIAKKIVTAHGGNI
                     TATSNEERTVFTVTLPNQDGTVG"
BASE COUNT         1336 a         1445 c         1607 g         1402 t
ORIGIN      
        1 tatccatcat ggggcttctt ccgtcgcctg gacgcattgt gggaggcagg gttatgtttg
       61 gagggactga ccttgtagac ctttctgagg cagaaatgcg aacgattcgt ggcaaggaga
      121 tcgccataat atttcaggat ccgatgaccg cgctcaatcc tgcctatcga gttggtgaac
      181 agatagcaga atctttgcgg gtgcatggta tggttccagt atctgcaggt gacaccggaa
      241 ggttatgcgg agatgggggc gggttgggcc cgagaatgag gaagacacga tcgcgtaagc
      301 tgtctgccgc cgaagaacgt gagtatgtat tcgagctgat gcagagtgta ggaataccca
      361 tgcccgagca gagacatctg gattatcctc accagttcag cggaggcatg cagcagagga
      421 taatgatcgc gatcgccttg gcttgcaagc cgaagcttct tcttgcagac gaaccaacta
      481 ctgcactgga cgtaaccatc caagctcagg tgctcgactt gatagatgag atcaacgaga
      541 ccaccggaac tgctgtcgtc cttgtcacgc acgacttggc tgtggcagca gatttctgcg
      601 attctatagc tgtgatgtat gcggggcaga ttgtggagcg agggccaaca gaagcggtta
      661 tggagggatc agctcatcca tatacccgcg gcctgctcag atccatgcac gtgctgggag
      721 agaggaagcc tctcaggccc ataatgggcg aagtcccgga tcttgcaaac ctgggcccgg
      781 ggtgcagttt cgctccaagg tgtgactttt cagaggatgt gtgttttgag agggctccgg
      841 atgtgagcat cgtggaaaag gggcatgagg tcagatgcca tcatcactcg ggcgcgttgt
      901 agcctggtgc gaatgatcgg tgagtgccag ttatcggcag tccggaatgg tctatggggt
      961 atgctcccgc gaaatgctca gaattggcga tctcaagccc catcgtactc attcatacag
     1021 ccttttacca atgtctctta aggtcttccg ccgactcacg cggcggcagg ccacacttga
     1081 ataattcacg cccggtgggg aggacacgca aaatcactgc tcaactggag tagaaaactc
     1141 tcttcacgcg gatgcctatc cggctgagca actcattggt tatcgttccg gcatcggcag
     1201 ctgcttgttc tgccgtgatc tccgcagaac cgtctctgcc gatcaatgtg gcgacatcgc
     1261 cgcgcttcac gtccgggatc ccggtgacgt cgaccatcag ctgatccatg catattcggc
     1321 ctataatggg tgcacggcat ccatggatca atactgagcc tctgccgccc gagagattgc
     1381 gtggaatccc gtccccgtac ccgattggga ggatggcgac acaagtatca cgctcggcaa
     1441 tgaagtggcg accatagccg acgctttccc cggctgtaac ggttctaaca agcgaaaccc
     1501 gtgccttcaa cgacaggaca ttttttatgt ccaggtggag cctggtctga tcatggggcg
     1561 agctcagtgt gccgtacaat gcaatcccga ctcttgcata atcgcattta agcattggat
     1621 agttaaggag gccataactg ctttgaacgt gtatcttggg aagggtgacg ttttgctccc
     1681 ggagcgtctc caggagatgg taaaacctat ctatctgcat ttctgtaaat tgagcgtctt
     1741 ccggttccat gctatctgag acggaaaggt gagtgtagat tccgtcaata caaagattct
     1801 tgaggccgaa gatgctggca atctccgata catgatccca gggctctccc atacggtgca
     1861 tgccggtgtc cactttgatg tggacgtgta ttggtttccc gcaagcattg aggcgcacag
     1921 cgtgttctcg atcgacgact gtctgcgaca aacggtagcg ggcgagctcg ccagctcttt
     1981 cgggcacagt gtagccgagt atcaggattg ttcctctgat tccatgtttt cgcaggcgaa
     2041 ttccttcatc gatagcggca accgcgaagg catcgattcc tatctgattc agatgttttg
     2101 caacttccac atcgccatgc ccgtatgcat tgcctttgac ggcagccatt agtttacatc
     2161 catccggcat agctttgcgc aggacttgga cgttgtggct caggcaggtt aggtcgatct
     2221 cagcccacgc cctgtccata gtggcgtgtt caggagcgag ctctctgttt tgaaagactc
     2281 gcagcaacaa caggatcgct tctgcggcag caagggaaga tactgctacg gcaagaaaat
     2341 ggatgacgct gttagctatc agccatcgtt tgaggcccgc ggctttggca agccatcgaa
     2401 cgagtatgat catcatggga tggactaaat agatgagcgt agagatgtcc cgcagctctt
     2461 tgcgcgtttt gccattgatg tgtagcaggc tctgaaaaag gagagccgcg cagggcagga
     2521 gcgtaaggta catgctatcg tgccttggga tcccgcaggt gtgaaggaga agcgcttctg
     2581 ccagcataag aagaaacgca actgccagcc ccgtaaagca cgcctttggc tgcaggcgcc
     2641 tcgctggcgg ggccatcatg gcgccaagca ctagaaagac cggagcgaag aaaagtccgt
     2701 tccgcgtgta gtccgaaact gtgaagactg atgcatagaa cgattgcagg aatggcactc
     2761 tttctgcaat tccgtagtag ctgtcgccga acaggccgac caggtagagg atgaaggcta
     2821 tgccaaaggc gtggccaggg ccgagccttt tcagcaacag ccaggcaatg gtcgcgccga
     2881 tcatcgctgc cggcagatac cataagtgat aaaacgtgcc gtcaaacaca atgtccttca
     2941 gcagttcttt ggccagatgt tcagttctga aggttcccgc atagatgttg acagggagat
     3001 acagcaaagt tgaaatggcg tacaacgcgg ccgttttcct cagaaagcga aacagccttg
     3061 cgcggtccgc ttcgctgtct gtcgcctttc cacgaaacag gaaaaatcct gaggtcatga
     3121 agaagaacgg cactgctaca cgggcaatta tccttgtcag gatgaaatca gcggtcccgt
     3181 tcactgacga caggggggaa gtgtggttag ccactacgag aaatgcagcg attattctga
     3241 agtaatcaat accggggaac tcttcctgca gattcatccc aaatgcctcc ataatgtcac
     3301 cacaaagcca tcatcgttgt tcccggatac ctgaaaaggg attctgtccg gcagttcgat
     3361 ttcaacctcc tgacctggtt cgacggggac atgaaaaatc tcgaagtcgt agttgtcgtg
     3421 cttgatccga agatgtgctc cgtcgaaggg aaagtgcttg aggcagtcag tatactcctc
     3481 cagcgtaaac tcatttcccc tgatcagttg ggaatgggga tagccaacgt agcgaaaatg
     3541 ccagggctcg tgtgcaattt gggtgatgga ttcccgtcca gccgggtagc gctggatgaa
     3601 tccatactgt actgacagct tccggaactc ttgacagatt ccggtgtaag ggaagtatgg
     3661 ccggatgaaa tcgatgtccg gcctgttttc tcccaaatcg attgctagtc cggtctggtg
     3721 ctcgctgcat cccacagttg cgacgtattt cttcgtaaac tccttcccgt tttcgcgcat
     3781 ggaatcggcg tatatagcct gctgttcctg catcgtgcga taaccgctga ctggtacgat
     3841 ttgatcctcg cattgcaaga gagcagccac ttctgccagc atccttgctg tttgcctttc
     3901 tagaacgatc tcgggaaagg cagggcgaat aggcacaagc acttcatcgg ctggtgtcgt
     3961 cgcaagtgga tgagagggat tgacgaggat gaggttgccc ttgccaatgt cctctgcata
     4021 gagtttcagc gttctcatcg gtcgatcgcc agccttataa gcgtgtctac aatctgctca
     4081 aagctcattc caatcccctt tagcatgttg ggataccggc tatgggtggt gaaaccgggt
     4141 atggtgttga cttcattgaa gacgatatct tcttcaggag tgaggaacat gtcaactcgc
     4201 gcgtaccccg aacactccag agccctgtag atgacgatag ctgtttgctt gatgcgtgca
     4261 gcagtctcca catcgatccg cgcaggcata tggatcttcg aagttttcaa cgtatatttc
     4321 tcggtgtaat cgaagaagcc gttgcagagc tcgatctcgt ccacctctcc aatggtgagt
     4381 acgtcattgc cgaggacagc acagccgacc tcgaatccat aaattgattc ctctattact
     4441 acttttctat cgtgttcgaa ggcagcactg actgcacagg caagcccgct ttcctgggaa
     4501 accttagtaa tgccgaagga ggagccggca tttgccggtt tcacaaagag tgggtatttc
     4561 aggtgcgcaa tccgcttaag cagctccgct tcggagacgt aagagtgcag cacaatcgat
     4621 ggaggtgttt ttactccagc caggctgact attctgtgag ctatgtcctt gtccatgcac
     4681 atggctgagc tcaatacgcc acatccaaca caggggatgc ccgccatttc cagtaggcct
     4741 tgaactgttc cgtcctcgcc gttcttccca tgtagaaccg gaaaggcaac gtcgattcgg
     4801 gtcgtggtta ccttgttgcc gtgaaattca agtatcccat gggtatttcg atcgggagaa
     4861 atgatggccg gaacggagac gctgtgatcc atccacgtat tgtccttgat ctgctccaca
     4921 ggaccggtga atctcatcca agctccctgg cgggtaatcc cgagcagtat caagtcgtat
     4981 tcttcagggt tgaggttggt gattaccgaa taggccgact gcagcgatat ttcatactcc
     5041 gtggaagatc cgccgaatat cacggctact cttgttcgtt tcatgttcat gactccttgt
     5101 ctctacggtc ttggcgttta cgtggtgttc tgcgggttga cagcggcggg ggatgaaggc
     5161 ctccgcaagc tgtttccttc tcaaacacgc aacgtaatct atgaagtaga aacgcctgcg
     5221 tgattcgtta atctaacctt atccgaatca ggcaggctaa aaagtaggct ctgcttgtag
     5281 aaatcttaag aaatacttac ggacgactca ttgttctgtt aatctcggcg gccggtgcat
     5341 ttgattgtgc tcaaggtggt ttcacccgat tggcgaattg ttatgggata aaagtgtgtg
     5401 ggggaagcca acacaagaca ggaaagcaac atgggaggag agatatgagg aacgagatgg
     5461 gtgagccgat agccaatcac gggtatggct ccgcatgtct cgcgcaattc ggatgacatg
     5521 gcgaattgcg gcgacgtgat tcgcgtcgcg agtgcatggt gcagagcggg ggcagttgca
     5581 tcatgattat ccaactgttc catcttgatt cgggagcgta acggtgaaaa ccgttcgttc
     5641 ctcgttgctt gtggctgtga tgttgcctcc atgtgcagta acgatcttct tggcgatggc
     5701 aaggcccaat cctgcaccgc cagaattcgt agaacgagag gcatcaagcc ggaagaactg
     5761 gtcgaagatc gtctctagct tggcggatgg
//
LOCUS       DAOSAI010000120         5777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8819_length_5777_cov_3.736456, whole genome shotgun sequence.
ACCESSION   DAOSAI010000120 DAOSAI010000000
VERSION     DAOSAI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8819_length_5777_cov_3.736456"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLM83_05300"
     CDS             <1..184
                     /locus_tag="PLM83_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70858.1"
                     /translation="HQNVVVRLTQSGKVLAESKYPVVRSQQMQAIGVKATNASFDPYA
                     PLSLELAMRQSNMGEE"
     gene            190..633
                     /locus_tag="PLM83_05305"
     CDS             190..633
                     /locus_tag="PLM83_05305"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOK70859.1"
                     /translation="MTAQSVGKGQISQHKAFTCIVCPRSCDLTAELVEDGWRISGNRC
                     PMGRDFALKEVNNPTRVVCSTARTTDRSCPLLPVRTDIEVPLEDVPRVMAEIIKAVVD
                     VSTGVDMGDVIIHNVAGTGANVIASASATMPMGVSSIETRTDRFH"
     gene            605..2161
                     /locus_tag="PLM83_05310"
     CDS             605..2161
                     /locus_tag="PLM83_05310"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOK70860.1"
                     /translation="MKQGLIVSIDCGTQSARALLFDCRGSLVAKGKAEFEPYFSTQPG
                     WAEQDPEVYFDAICKACHDLASSAPDDWGRIVGVVLTTQRDTCVVVDENGNVLRPAML
                     WLDQRMAECNKPYPFIYRLAFGAVGMLETAELTRKKCKSQWIRENEPDIWDKMNKFLL
                     LSGWLNYKLTGNMADSVANQIGHVPFDYKHKRWPVSKLDYRWYMSGLSRRHLPQLVRP
                     AQVIGTITPEAAEATGLPVGTPVYAGGSDKGCETLGTGCLNPTSACISLGTTATLQIT
                     TEKYMEPIKFMPPYPAVVPGHFNPEVQIYRGYWMINWFKQEFGYREVLIAEQRGVAPE
                     VILNELLHETPPGCMGLMLQPYWGAGLRMPEARGAIIGFGDIHTRAHLFRSIIEGVAY
                     ALRDAADKVEKVSGTKIERIMISGGGAQTDSICQITADVFNKPVLRGETYEGSGLGAA
                     IIGFCGAGYYSSFDDAVANMVHYEAHVQPDPPTARTYDQLYKCVYKKMYSRLKPLYEE
                     MRAITGYPES"
     gene            2178..3899
                     /locus_tag="PLM83_05315"
     CDS             2178..3899
                     /locus_tag="PLM83_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOK70861.1"
                     /translation="MSNKKPFEPDWYEGPIPPKSYRSIFKWGGADEYKHPNLHLYELM
                     KEVFEMTDEDFRAPVNLGLEEVSFDVPITLSGEHLKAITSIVGTENVATDDYSRLRAS
                     YGKMIYDNIRLREGIVENLPDIVVYPRHKQDVSEIIGYCNRHKIAVYPRGGGSGVMRA
                     TECVHGGISLDLTKHMNRILTLNEVNHTITVEPGIYLPDLEARLNKAPEFFGASRRYT
                     AGYLPQSFIGATVGGSVVTRGAGQNSTYYGKIEDLVLCQEYVTPAGDIKTKEFPRAAV
                     GPSIDQIMIGSEGTYGVLVSVTLKIFRYTPETTQRFSYIFPTWSNGLAASREMMQSRF
                     GYPSVLRISDPEETDVALKLYGVEDTILDKMMDARGYKRMERCLMLGMSEGEENFAKN
                     VKRQINRIAKRYGAMNTTGYVAKSWEKGRFTDPFMREDLHDYGIIIDTLECAVAWDKI
                     ESLWAETRKVCKARPKTVVMSHSSHFYPQGTNLYFIFIGRFETIDEYLDYQASIIDSI
                     RANGGALSHHHGIGKMLAPWYQDAMGENHVAVLRALKRHFDPNNIMNPGGTLALDLEE
                     SFRRPIE"
     gene            3949..4671
                     /locus_tag="PLM83_05320"
     CDS             3949..4671
                     /locus_tag="PLM83_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK70862.1"
                     /translation="MIDKLSPVPFYIQLRDGIKRNVIEGVWKVGEQIPTENELMEKYE
                     VSRATVRNAISSLVNEGFLVKQRGRGTFVARQQPSMAFEPLISFAYNVESMGASSESQ
                     VVENSIIVPDEELLRLLRWKEPKECLYVKRVRHGDGIPLAIEESFFNDSTAKFLKLKD
                     LSGSISRLLLEELRISISRVEQIVAARKATAEEKKLLNLDKEPQVLEMERWILLEDEE
                     EPFYYLRFIMRGDLYSLTPVPR"
     gene            complement(4831..5430)
                     /locus_tag="PLM83_05325"
     CDS             complement(4831..5430)
                     /locus_tag="PLM83_05325"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK70863.1"
                     /translation="MHNDYMVPVADKRQLILDAAQHVFAEKGFHTSTMDAVAEAAGVA
                     KGTIYLYFKGKNELLIELVDDRTRRLTDMILNGIASAEDVFGKVFAVIEAHFQFYSIH
                     SEFMLLFSGQLGLLSPEMEERLVTGTAALINMVCGVIEEGMALGVISSDVDPMRASFA
                     LEGMIHSVAYQWLSAGKSVSVSVVSHEVYTLFTHGIACP"
BASE COUNT         1539 a         1504 c         1522 g         1212 t
ORIGIN      
        1 acaccaaaat gtcgtggtcc ggctaaccca aagcggcaag gtcctcgccg aatcgaagta
       61 tcccgtggtg cgatcccagc agatgcaggc cataggggtg aaggccacga acgcaagctt
      121 cgatccatat gccccactct ctttggagct ggcgatgcgc caatcgaaca tgggagagga
      181 gtgatcatta tgacagctca aagcgttggc aaaggccaaa tatcccaaca caaggccttt
      241 acatgcatag tctgtccgcg cagttgcgat cttacagctg aactagtgga agacggctgg
      301 agaatatcgg ggaacaggtg cccaatgggt agggattttg ccctaaaaga agtaaacaat
      361 cccacgagag tcgtgtgttc tactgcgaga acgactgacc gatcctgtcc gctccttccc
      421 gtaaggaccg acatcgaagt gccgctggag gacgttcccc gtgtaatggc agagatcatc
      481 aaggctgttg tagatgtgag cactggagtg gacatgggag acgtcattat ccacaacgtg
      541 gcaggcactg gcgcgaacgt aatagcgtcg gcaagcgcta ctatgccaat gggggtatcc
      601 tcaattgaaa caaggactga tcgtttccat tgattgcgga acccaaagcg cacgagcgct
      661 gctatttgat tgtagaggaa gcctggtggc aaagggcaag gcggagtttg aaccctactt
      721 ttctacgcag ccaggctggg cagagcaaga tcctgaagtc tatttcgacg ccatctgcaa
      781 ggcatgccat gacctggcat caagcgctcc tgatgattgg ggtcgcattg taggagtagt
      841 cctgaccacc caacgcgata cctgcgtagt cgttgacgag aatggcaatg tgctgagacc
      901 ggccatgctc tggctcgatc agcgaatggc cgaatgcaac aagccctatc cttttatcta
      961 cagactcgcc ttcggcgcag tagggatgct cgaaacagcg gagctcactc gcaagaaatg
     1021 caagtcacag tggatccgtg aaaatgagcc ggatatttgg gacaagatga acaagttcct
     1081 gctcctttcc ggatggctca actacaagct caccggaaac atggcggact cggttgcaaa
     1141 ccagatagga cacgttccat tcgactacaa acacaaaagg tggcccgttt cgaagttaga
     1201 ctacagatgg tacatgagtg gtttgagccg gcgacacctt cctcagctcg taaggcccgc
     1261 gcaagtgata ggcaccatca cgcctgaagc agcagaggcc actgggctac cagtaggcac
     1321 cccggtatat gccgggggat ctgacaaagg atgcgaaacc ctcggaactg gatgcctcaa
     1381 tccaactagt gcatgcatca gccttggaac tacagcaaca ttgcagataa ctaccgaaaa
     1441 gtacatggaa ccgatcaagt tcatgccgcc ttaccctgca gtagtgccag gacacttcaa
     1501 ccctgaagtg cagatatacc gcggctattg gatgatcaac tggttcaagc aagaattcgg
     1561 ataccgcgaa gtgctcatag cagaacagcg tggagtcgct cctgaggtaa tcctaaacga
     1621 gctactccat gagactccgc cgggctgcat gggcctgatg ctccagccat actggggcgc
     1681 ggggctcagg atgccagaag cccgcggcgc cataatcggg tttggagaca ttcacacgcg
     1741 tgcccatctg tttcgatcga taatagaagg cgtagcatac gctctacgag atgcggctga
     1801 caaggttgaa aaagtctccg gcacaaagat agaacggata atgatatcgg gcggcggcgc
     1861 gcaaacagac tccatatgcc agataacagc ggatgtattc aacaagcctg tgttgcgcgg
     1921 cgaaacctac gagggatctg gacttggcgc tgcaatcata ggattctgcg gcgcgggata
     1981 ttacagcagc ttcgatgatg cagttgccaa catggttcac tacgaagccc acgtccaacc
     2041 agatccaccg actgcaagaa catatgacca gctatataag tgtgtataca agaaaatgta
     2101 ttcgcgcctc aagccgctct atgaggaaat gcgcgcgatt accggatatc ccgaaagcta
     2161 ggaagaggtg atcaattgtg agcaacaaga aaccgttcga gcctgattgg tatgaagggc
     2221 caatccctcc aaagtcatat agatcgatat tcaagtgggg aggcgccgat gaatacaagc
     2281 acccaaacct tcatctctat gagcttatga aggaagtatt cgagatgacc gatgaggatt
     2341 ttcgcgcccc ggtcaactta gggcttgagg aggtcagttt tgatgtaccg ataacgctct
     2401 ccggcgaaca cctcaaagcc atcacaagta tcgtcggaac ggagaatgtc gcgacagacg
     2461 actactcgcg actcagagct tcatatggca aaatgatcta cgacaacata cgtctgcgtg
     2521 aaggaatcgt cgagaacctc ccggatatcg tcgtatatcc caggcacaaa caggatgtgt
     2581 cggagatcat aggctactgc aacaggcata aaatagcagt ctacccgcgg ggcggcggga
     2641 gcggagtcat gcgggccacc gaatgcgtac atggcggaat atctctggat ctcacgaagc
     2701 acatgaaccg catcctcacc ctcaatgagg taaaccacac tatcacagta gaacctggca
     2761 tataccttcc cgaccttgaa gcgcgactga ataaggcacc cgagttcttc ggagcctcca
     2821 ggcgctatac cgcgggttat ctgcctcagt ccttcatagg cgccaccgtc ggcggttcag
     2881 ttgtgacccg gggagcaggg cagaactcaa catattacgg caagatagaa gacctcgtcc
     2941 tctgtcagga gtatgtcacc cctgcgggag atataaaaac aaaggagttc ccgagagcgg
     3001 cggttggacc ttccatcgac cagataatga taggcagcga aggcacatac ggggttctcg
     3061 tatccgttac cctgaagata ttcaggtata cgccggaaac aacacagcgt ttcagttaca
     3121 tcttcccaac gtggagcaat ggcctcgcag catcccgcga aatgatgcaa agcaggttcg
     3181 gatatccgtc ggtgctccgg atctccgacc ccgaagagac tgatgtggca ttaaagctgt
     3241 acggagtaga agacactatc ctcgataaga tgatggacgc tcgtgggtac aagcgtatgg
     3301 agcgttgcct aatgctgggg atgtctgagg gtgaagagaa tttcgccaag aacgtaaaaa
     3361 ggcaaataaa caggatagcg aaaagatacg gcgcgatgaa taccacggga tacgtcgcca
     3421 agtcatggga aaaaggccgt ttcaccgatc ccttcatgcg ggaggacctc catgattatg
     3481 gaatcatcat tgatactttg gaatgcgccg tcgcatggga taagatcgaa tccttgtggg
     3541 ctgagaccag aaaggtctgc aaggccaggc cgaaaacggt agtaatgagc cactcgtccc
     3601 acttctaccc tcaaggaacg aacctctatt tcatttttat cggacgtttt gagactatcg
     3661 atgaatacct cgattaccaa gccagcatca ttgatagcat acgggccaac ggcggagcgc
     3721 tcagccatca ccatgggatc ggaaagatgc tggcgccatg gtaccaagac gcaatgggtg
     3781 aaaaccacgt tgccgtgctc agagcattga agcggcattt tgacccaaac aacatcatga
     3841 accctggagg aaccctggcc ctcgatctag aagaatcgtt ccgcagaccg attgagtagg
     3901 gcattatgtg cccttcgggg ctagtgagca gtatggcgag ggttgtgcat gatagacaaa
     3961 ctaagcccag tcccttttta tatccaattg agggatggga tcaagcggaa cgtgattgaa
     4021 ggagtttgga aagtgggaga gcagattcct accgagaacg agctgatgga gaagtatgaa
     4081 gtcagtcgtg ccacggtgag aaacgccatt tccagcctgg tcaatgaagg ctttcttgtg
     4141 aaacagcgag gccgcggaac attcgtcgcg cgccagcaac catctatggc tttcgagccc
     4201 ctgataagtt tcgcatacaa tgtagagtct atgggggctt cgagcgaaag ccaagttgtg
     4261 gagaacagca tcatagttcc cgacgaggaa ctccttcgtt tgttgagatg gaaagagccc
     4321 aaggagtgtc tttacgtcaa gagagtgagg cacggagacg gcattccctt ggcaattgaa
     4381 gaatcgttct tcaacgattc cacagccaag tttctcaagc tgaaagatct ctcaggatcc
     4441 atatcccgcc tcctccttga ggaattgaga atatcgatat cccgggtcga acagatagtc
     4501 gctgcccgca aagccacagc ggaagagaaa aagctgctca acctcgataa agaacctcag
     4561 gtgcttgaga tggagcgatg gatactccta gaagatgaag aagagccctt ttactatctg
     4621 agattcatta tgcgtgggga tctgtactcg cttacaccag tccccaggtg ataatctcgg
     4681 agaggcaatg tttcgatgcc agcagcaagg cgcctcgcag ccctcacttt ggggacgcgt
     4741 tggcgcctct tggcgctctg tgggacgatc acccctgtag cccattttcg acgaagcagc
     4801 gacatcgcct gtaaggcgcc gatgcgatgc tcaggggcaa gctatgccgt gggtgaacag
     4861 cgtatacact tcgtggctca caacgcttac ggaaacgctc ttgcctgcag aaagccactg
     4921 atatgctaca gaatgaatca tgccttcaag ggcgaaggaa gctctcattg gatccacgtc
     4981 tgaactgatt acgcccaaag ccatgccctc ctctatcact ccgcacacca tattgatgag
     5041 cgctgctgtg ccggtcacta atcgctcttc catttctgga gaaagcaatc ctagctgccc
     5101 cgaaaagagc agcatgaact cgctatgaat agagtagaac tggaaatggg cctcgataac
     5161 ggcaaaaacc tttccgaaca cgtcctcagc cgatgcgatg ccattgagta tcatatccgt
     5221 cagccgtcgc gtccggtcat caacaagctc aatgaggagt tcgttctttc ccttaaaata
     5281 aaggtagata gtgcccttgg ctacacctgc agcctcggcc accgcatcca ttgtggaagt
     5341 atgaaagcct ttttcggcaa acacatgttg tgcagcatct agtatcagtt ggcgcttgtc
     5401 agccacgggc accatataat cgttgtgcat attgcgcctc cctcctcacc accgcctgca
     5461 tcctcccgcc attcggattt tcgaacactg caggataggc aatggccagt tcgattgtta
     5521 ctctagatta ttcccgatct ttatatttcc cccttccaca cggcattccc ttgttgcata
     5581 gcacaaagag agccgaaccg ttacaccggc acgctctctt tgcggcaaca ttcgaaagcg
     5641 cgggggcgct ccgccaccgg cccgcagacg cctgcatgct atgtgcgatt cgccactgtg
     5701 tgcccaaaca tctcccgaaa aatctgctgc tctaacgaag gccgggcaac catggctcaa
     5761 aagggacgcc gagacat
//
LOCUS       DAOSAI010000121         5768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8830_length_5768_cov_4.046911, whole genome shotgun sequence.
ACCESSION   DAOSAI010000121 DAOSAI010000000
VERSION     DAOSAI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8830_length_5768_cov_4.046911"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLM83_05330"
     CDS             <1..330
                     /locus_tag="PLM83_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOK70864.1"
                     /translation="NRVLLLTDGMANVGVTNPAALSAKARAMLEQGVSLSTVGVGLSF
                     DEDLLIALAEAGGGAYYYIKDADEIPNVFSAELKGYKPSSRCLFGPARRRTPRITRSS
                     VIHRRAP"
     gene            complement(793..1326)
                     /locus_tag="PLM83_05335"
     CDS             complement(793..1326)
                     /locus_tag="PLM83_05335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK70865.1"
                     /translation="MQENLLPLVSIELENGAVIEAELYPDVAPNTVNNFISLVSKGFY
                     DGLTFHRVIPGFVIQGGCPKGVGTGGPGYSIQGEFEDNGFANDLKHVPGVLSMARAAH
                     PDSAGSQFFIMVGTSPHLDGGYAAFGKVVSGYEEVERIAKIPCDRSDKPLSPVRMKKV
                     TVELFGVEYPEPETVAG"
     gene            1926..2789
                     /locus_tag="PLM83_05340"
     CDS             1926..2789
                     /locus_tag="PLM83_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOK70866.1"
                     /translation="MEEGDVKSLASAMGKRAVNIGMKNDSIKLLRSAMKGRHKSLIAV
                     EGIWLNAKALTARARIETLYVCPAMIHTPEGADLLSRLVDQTMGEICIISENVYHRLS
                     EPKSDEGVISLCRLPEITLNSVRPRTSDLVLVLDGLESPGNVGTIIRTAEAAGACAAV
                     ICHGQANITNPRTVRSSLCSVLSLPVLEADMEDIIRWLRMHEFALYFADASGGVVYSD
                     IEYAPRSALVVGNEKYGISPDWYETEHLSVYIPMMGTVDSLNAGVAASILAYEIRSQF
                     GHTRARSHMKA"
     gene            complement(2867..3649)
                     /gene="csb2"
                     /locus_tag="PLM83_05345"
     CDS             complement(2867..3649)
                     /gene="csb2"
                     /locus_tag="PLM83_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HOK70867.1"
                     /translation="MIGIVGVRCSVEPCFRPPVEETLLVGEMCRRAIMSQYGKAHGGA
                     VSPVLSGKDQSGKVLSGHQHAFYLADDGDGDGLIDTVFVYSVRGFNSLEIDAIARVRE
                     VYLGHYCEPMELRLSGVANMENTAQLGRIFGPSRVWISAVPYVMTRHPKRTRAGETKL
                     TKNGRQQDGPEEQIAREWDQRVHDNCSLPLLIDMQLLGATASRRVYRIVRAYGNAPDP
                     VKRAFKFKLMFDGPLMGPVALGYACHFGLGLFVPETATSPLP"
     gene            complement(3646..3789)
                     /gene="csx15"
                     /locus_tag="PLM83_05350"
     CDS             complement(3646..3789)
                     /gene="csx15"
                     /locus_tag="PLM83_05350"
                     /inference="COORDINATES: protein motif:HMM:NF040560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx15"
                     /protein_id="HOK70868.1"
                     /translation="MGHFPACVRIRPVAGSVPTQFEVAEIINLEAVRQEARTRRFPGS
                     ANK"
     gene            3781..4179
                     /locus_tag="PLM83_05355"
     CDS             3781..4179
                     /locus_tag="PLM83_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HOK70869.1"
                     /translation="MPHASMKFGHYQRRCVVQTGLVHYTDQEKTIHIDFDNALIARTY
                     DSLFELRKTLMASKTPPPTSDENRCTQCPYAEICMPDEIAFMSTAVSGNLATDEDAVD
                     NAEVRRLVPARDDRIPLYVQTQGAVVSKRD"
     gene            4492..5067
                     /gene="cas1"
                     /locus_tag="PLM83_05360"
                     /pseudo
     CDS             4492..5067
                     /gene="cas1"
                     /locus_tag="PLM83_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
     gene            5072..5362
                     /gene="cas2"
                     /locus_tag="PLM83_05365"
     CDS             5072..5362
                     /gene="cas2"
                     /locus_tag="PLM83_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOK70870.1"
                     /translation="MRRRFIVCYDISDPKRLRKMYRTMRGFGDPLQKSVFTCDLSSKE
                     RAHMVKAIPRIIDCQEDSVLIIDTGGLDAKRESLEYIGKAITPDEGRAAIIV"
BASE COUNT         1478 a         1670 c         1399 g         1221 t
ORIGIN      
        1 aaccgtgttc tgctgctcac ggacgggatg gctaacgtag gtgtaaccaa ccccgccgcg
       61 ctctctgcca aagcccgcgc aatgctggaa cagggagtat ccctctccac cgtcggagtc
      121 ggcctgagct tcgacgaaga cttgctgatt gccttggccg aagctggagg cggagcctac
      181 tactacatca aagatgccga tgagatcccc aacgtcttct cagcagaact caaaggatac
      241 aaaccgtctt cacggtgcct ctttggccct gcccgaaggc gaacaccccg tattacgcgt
      301 agctctgtca tacacagacg cgctccataa tctagctcat actaccctcg aagtgacggc
      361 gaaacttgct gcaatcctcg gagcagccga gccgcaggag ccaaacttcg acgtcatcaa
      421 aaccgtcgag ctcatacagc caaggacgaa agcgccaaag ccatggacag ggatgattac
      481 aaggaggccc gcgcaatact ggaaaatcgc atagacacct tgcgaaagct caccgaggag
      541 atcagcaaaa ccaccccacg acatacacta agcgaaacgc gcaagaacat gcggtcccaa
      601 agctaccaaa taaggcacag ccaatctgat tacggatcga agaaggatcg ctgaaccaga
      661 ccggcagcgc gatttctgca gacaaactcg ccacgaggat cataggcagt atcatacaca
      721 aacagctcta caagccgcct tatggaggcg tgcagggcta gttcttcagt cttgcaagcc
      781 tgggcaggcc ccctacccgg ccacagtctc aggctccgga tactccaccc cgaacagctc
      841 tacagtcacc ttcttcattc tcacaggcga cagcggctta tcgctccggt cgcacgggat
      901 tttcgcaatg cgttccactt cctcgtagcc cgacacgact ttcccaaaag ccgcataccc
      961 gccatcaagg tgcggcgatg ttccaaccat gatgaaaaac tgcgaacccg cagagtcggg
     1021 atgcgcagcg cgcgccatgg aaaggactcc gggtacatgc ttgaggtcat tcgcaaaacc
     1081 gttgtcctca aattccccct gaatactgta cccggggcct ccggttccga cccccttggg
     1141 gcatccgccc tggataacga agcccgggat cacccgatgg aaggtgagtc catcgtagaa
     1201 gcctttgctc acaaggctga tgaaattgtt taccgtgtta ggcgcaacat ccggatacag
     1261 ctctgcctct attacagctc cattttcaag ttctatggaa acaagtggta acaggttctc
     1321 ttgcatgtgc cttcctcctc gcacaacata atggtaaacc tgccatggct agatcgatca
     1381 caagcaccat cataacagct gaagcgcggg aaatgaagct cgacggccca aagctgcatt
     1441 tcctggatct ggtccaatca gaatcttctg atacgccata ccacgttcag aggtgtatcc
     1501 ggcccaacgc cgccaccttc atcaacttga gcaggaataa tcgaagcttt gccatcataa
     1561 cactgctagc ccgacctacg atggtcacga caacgggggc gctcagagtt ggccacttag
     1621 actgcacttg ccatgcaaat agccgtgtaa cagcaatccc caaatgtgat ttgattctgc
     1681 tattgctgaa gcagcatcaa gcaaggggag tcgcttcaaa tggaccacct cgagccacac
     1741 aaatgattgc cacggcgacc tcgtgggaat atagcagatg agccgttaac ctgtccccta
     1801 tggtagaagt ggcccgatcc aagacacttg gttgtctgct atactacata cgtgaaggaa
     1861 tcatggttca tatgcgcagc gatacactcc gcctagtatc gctgatctga atggggggcg
     1921 ttgatatgga ggagggcgat gtcaaatccc ttgcctcagc tatgggcaaa agagccgtca
     1981 atatcgggat gaaaaacgac agcatcaaac tccttcgttc agccatgaag ggccgccaca
     2041 aatccctcat tgcagtggaa ggcatatggc tgaatgcaaa ggcgctcacc gcccgggcac
     2101 gcatcgaaac attgtacgtc tgccctgcaa tgattcacac gccagaaggg gcggacctcc
     2161 tttccagact ggtcgaccag acgatgggtg agatctgcat aatctccgaa aacgtctacc
     2221 acaggctgag cgagccgaaa tcggacgaag gcgtaatctc actctgcaga ttaccagaaa
     2281 tcacgctcaa cagtgttcgt ccgcgcacta gcgacttagt gctcgttttg gacggtctgg
     2341 agagcccggg caatgtgggg acgataatac gcacagcaga ggccgccggc gcttgtgccg
     2401 cagtcatctg ccatgggcaa gcaaatatta ccaatccccg aacagtgcgg tcgagcctgt
     2461 gcagtgttct gagcctccct gtattggagg cggatatgga agacatcata cggtggctcc
     2521 gtatgcacga attcgcgctc tacttcgcag acgcctctgg cggcgtcgtc tacagcgaca
     2581 tagagtatgc cccgagatct gcattggtgg tcgggaacga gaaatacggg ataagcccgg
     2641 actggtatga aaccgaacac ctgtcggtgt acatcccgat gatgggcaca gtcgattccc
     2701 taaacgctgg cgtcgccgcc tctatactcg cctacgaaat tcgcagccaa ttcgggcaca
     2761 ctcgcgcacg gtctcatatg aaagcttaac ggtgtgaagg atcctattat cccgtgcatg
     2821 catctttgtc ttctctcctc caggccatac gctcgggctc tggcaatcaa ggcaatgggc
     2881 ttgtagctgt ttcaggcaca aacaatccca atccaaaatg gcatgcatag cccaaagcca
     2941 caggccccat caaagggcca tcgaacatga gcttgaattt gaaagctctc ttcaccggat
     3001 ctggggcatt gccataggct cggactattc gatatacccg ccttgaagca gtcgcgccaa
     3061 gcagctgcat atcaatgagc agcggcagac tgcaattgtc gtgtacacgc tgatcccatt
     3121 cccgggcaat ctgctcttca gggccgtcct gttgtctgcc gttcttggtt agcttggtct
     3181 ctccggcccg tgtcctcttc gggtggcggg tcatgacgta aggaacagca gaaatccaaa
     3241 cacgagaagg cccaaagatc ctgcccagct gcgcggtgtt ctccatgttc gccacgcccg
     3301 acagcctgag ctccatgggc tcgcaataat gtccaagata gacttccctc actctggcaa
     3361 tcgcgtcgat ttcgagagag ttgaaccctc gaacgctata cacgaatact gtgtcaatca
     3421 acccatctcc gtcgccgtca tcagccaaat aaaaagcgtg ctgatgaccg gagagcacct
     3481 ttcccgactg gtcctttccc gacaggacgg gcgatactgc tccgccatgg gcctttccat
     3541 actgactcat aatcgcccgt ctgcacatct cgcccacaag caacgtctct tctacaggcg
     3601 gacgaaagca aggctccaca ctgcatcgga ctccaacaat gccaatcact tatttgcact
     3661 cccaggaaac cgtcttgtcc tcgcttcctg cctcacagct tccaagttga ttatctctgc
     3721 tacttcgaat tgtgtgggca cgctgcccgc cacaggccta atgcgcacgc aggcgggaaa
     3781 gtgccccatg cgtccatgaa gttcggccac taccaacgcc gctgcgttgt gcagaccggg
     3841 ctagttcact acacagatca agaaaagacc atccacatag acttcgacaa cgcgctcatt
     3901 gcacgtactt acgattcgct cttcgagcta cgaaagactc tcatggcctc aaaaacacca
     3961 ccaccaacct cagatgagaa caggtgtacc cagtgtccat acgcagaaat ctgcatgcca
     4021 gatgaaatag cattcatgag cacggctgtt tctggcaacc tagcaactga tgaagatgca
     4081 gtcgataatg ccgaggtccg acggctggtg ccagcgcgcg atgacagaat accgctctat
     4141 gtccagactc aaggcgcagt agtttcaaag cgcgactgat agatctgtct caagtctgca
     4201 tatatggcaa cgtgcaactc acgacacaat ccatacgcgc attttgcgca agagacatac
     4261 ccatctgcta tttttcccgt ggaggctggt tctacggaat taccaagtcc accatgggca
     4321 ggaacgtgac agttcgtaga gagcaataca aggccactgc tgacccgaac cgcgcccttt
     4381 ccatatcaaa gcgatttgtt gcagggaaaa tcaaaaactg tagaacactg ctccgtcgca
     4441 actgccccga ctgcgataca ggcgtcctag acaagatgtc ggagttatgt cgacaaagct
     4501 atgtctgcca acaacatgtc cactctcttg ggaattgaag gagcagccgc tagcctgtat
     4561 tttggaaact ttgggaccct gctcagcagc actaccggat tcgatttcac agctcggaac
     4621 cgacgtcctc ctaaagatcc tgtgaatgca gttctttcat atctatacag catgctggta
     4681 aaagacatta ccatcgtcgc cttggcaaca gggctggatc cgtatgccgg attctgtcat
     4741 caaccacttc aaggaaggcc agctttggcc ctcgacctca tggaggaatt ccggcctcta
     4801 gtttgcgact ccaccgccat caggcttatc aacacgtctg cgctcaagaa ctctgacttt
     4861 tcgcgattgg gcaatggcgt tatcatgtcc gacaacgcaa gacgcaaagt aatctctgcc
     4921 tacgaaaagc gcatggatac cctggtaacg catccgatat tcggctacgc catgagctat
     4981 cgcagagttc tttcggttca ggcgaggcta gtagcacggt ggttgctggg cgaaatcagc
     5041 gattatccac cattttgtac aaggtgatga tatgaggcgc aggtttatcg tctgctacga
     5101 tatttcagat ccaaaacgcc tgcgaaagat gtatcggaca atgcgcgggt ttggggatcc
     5161 attacagaaa tcggtcttca cgtgcgatct gtcatccaaa gagcgggccc acatggtaaa
     5221 agcaatccct agaatcattg attgccagga agactccgta ctgataatcg ataccggcgg
     5281 ccttgacgcc aagcgggaat ccctcgagta tataggaaag gccataacgc cagacgaagg
     5341 cagggcagca atcatcgtct gatacagcca gcctgcattc gaattgaggt aatccaaggt
     5401 tataagagaa gccaagactg caggtcatga taacatgttg tagaagaaat ccaagattca
     5461 aggggcttcc tttcaaaccc gcttcattgc caacaactcg agcccgagat gaagcccggc
     5521 gatgctctcc gccacggcta ccatagccct gcaacactaa gaaccgctat gaacaagcaa
     5581 cctcagactg cagacaaaac atcagtggat tccgcttcag cgacgctcac caggttacct
     5641 gcagattcta tggcgagcac aatctctcga taaattcctg ggattctcga gtccgcactg
     5701 agtgactccc aacaagtttt tgcgctatcg gttagcctgc catatgatgt ctgctaggcg
     5761 tagacacg
//
LOCUS       DAOSAI010000122         5746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8878_length_5746_cov_3.672641, whole genome shotgun sequence.
ACCESSION   DAOSAI010000122 DAOSAI010000000
VERSION     DAOSAI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8878_length_5746_cov_3.672641"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLM83_05370"
     CDS             <1..274
                     /locus_tag="PLM83_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOK70871.1"
                     /translation="MNDEERWAYVEDRSGIELLNDVIREISDFNQYDQVIFLGSDFRV
                     RLIEAILGEKVTSVVIGKSPIKEDVNIDVAEMISIIKAVRGGAEKA"
     gene            271..1191
                     /locus_tag="PLM83_05375"
     CDS             271..1191
                     /locus_tag="PLM83_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HOK70872.1"
                     /translation="MKHVVSVSIGSSDRNHSATVNLLGEDILVERIGTDGDMNRAVEL
                     ISELDGKVDAFGMGGIDLYMAGVGKRYVFRDAKRISQAAKKTPLVDGTILKSSLEYNA
                     VKYAAEVEKLPMAGKNVFVVCVIDRIGMGRAFEELGCNMVYGDLMFSTGMHIPLYSLN
                     SIYKLAPIFMPIMTQLPFSWLYPTGKEQNASRKRPKDKKPKFQKYYNDAHVIAGDFHY
                     IYKYLPEDISGKIVVTNTVTSKDVDELGRRGASALVTTTPEINGRSFGVNVIEAILVA
                     LIGKPVEDISDEDFTAMVQCLEIKPRIVRY"
     gene            1439..1915
                     /gene="greA"
                     /locus_tag="PLM83_05380"
     CDS             1439..1915
                     /gene="greA"
                     /locus_tag="PLM83_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOK70873.1"
                     /translation="MSDKEVILTPEGLHNLEKELEYLRTTKRRDIAARIKQAIEFGDI
                     SDNSEYEDAKNEQAFVEGRIAQLEKMLRNAKIIEGPDGDGDAVSVGSTVVLKDLNHGD
                     VEQYTIVGSAEANPSRNRISNESPVGRAVLGKTVGSIVEVNVPVGTIRYEVVQIAR"
     gene            1925..3409
                     /gene="lysS"
                     /locus_tag="PLM83_05385"
     CDS             1925..3409
                     /gene="lysS"
                     /locus_tag="PLM83_05385"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOK70874.1"
                     /translation="MDELGGQFEGAELGNELAHRKAKVEEWRSRGIDPYGSKFVRTHC
                     AAEIVEDFSELEGEEVSIAGRIMALRGHGKASFLDIVDASGRIQLYAKHDALGDELYE
                     LLGMMDIGDIIGVTGTVFRTRRGEISVAISSFRILSKALRPLPEKWHGLRDVDTRYRQ
                     RYLDLMVNPEVQRTFVLRTKIISAIREYLDSRGFLEVETPCMHAIAGGAAARPFITHH
                     NALDMELYLRIATELHLKRLIVGGFEKVYELGRIFRNEGISTKHNPEFTSIEVYEAYA
                     DYYDMMDLTEGLVAYVAETVLGTTEVEYQGDKIDFAPPWTRITMAEAVLKETGVDFLN
                     IEQTEAVAASRKLGLKVSDDATCGFCMSEAYEEFVESRLVQPTIVMDYPIEVSPLAKR
                     RADNPNLTYRFEVVCAGRELANAFTELNDPEDQLQRFSAQVAQREKGDDEAHMMDEDF
                     VTALEYGMPPTGGMGLGVDRLVMLLTDSPSIRDVILFPLMRPKE"
     gene            3427..4158
                     /gene="nagB"
                     /locus_tag="PLM83_05390"
     CDS             3427..4158
                     /gene="nagB"
                     /locus_tag="PLM83_05390"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOK70875.1"
                     /translation="MQVLVYSDYDEMSAAAARMIASKIGELPKPVLGLATGSTPIGMY
                     AELVRLHNEEGLDFSNVTTFNLDEYCGVDAEDPRSYHWFMKEHLFSHVNLDPEKAHVP
                     AGNSADPDGECARYERLIKEAGGIDLQVLGIGMNGHIGFNEPGSDPNSRTRVVRLAEQ
                     TIEVNRAKGGLNALPGYAISMGIGTIMEARQILLLANGAGKAEIIRRALTGPVTPDVP
                     ASLLQTHPNVVCMLDKPAASLLDLS"
     gene            4206..4281
                     /locus_tag="PLM83_05395"
     tRNA            4206..4281
                     /locus_tag="PLM83_05395"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4239..4241,aa:Ala,seq:cgc)
     gene            4563..4730
                     /locus_tag="PLM83_05400"
     CDS             4563..4730
                     /locus_tag="PLM83_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK70876.1"
                     /translation="MAYEINDNCIACGACKEECPTEAISEGDIYAIDPEKCIDCGTCN
                     EVCPVGAPNPA"
     gene            5004..>5746
                     /gene="pdxS"
                     /locus_tag="PLM83_05405"
     CDS             5004..>5746
                     /gene="pdxS"
                     /locus_tag="PLM83_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOK70877.1"
                     /translation="MAIGELVNSVDRSKLEFVEKLRGGVIMDVTTPEQAKIAEDAGAV
                     AVMALERVPADIRAQGGVARMADPALIEQIMDSVSIPVMAKVRIGHFVEAQILEAIGV
                     HCIDESEVLTPADERYHVDKHAFTTPFVCGCRNLGEALRRIGEGASMMRTKGEAGTGD
                     IVEAVRHIRAVNEDIRRLKNMPCEEIMAMAKEIGAPYELLCQVARDGKLPVVNFAAGG
                     VATPADAALMMQLGADGVFVGSGIFKSDDP"
BASE COUNT         1409 a         1231 c         1743 g         1363 t
ORIGIN      
        1 catgaatgac gaggaacgtt gggcttacgt agaagacagg tccggaatag agctgctcaa
       61 tgatgtgatt cgcgagatat cagattttaa ccagtatgat caggtgattt ttcttggctc
      121 agatttccgg gtccgtctca tcgaggcgat tctaggagag aaggtaacat cagtagtgat
      181 cggaaagtcc ccaatcaagg aggatgtcaa catagatgtg gcggaaatga tctccatcat
      241 taaggctgtg cggggaggcg ctgaaaaggc atgaagcacg ttgtcagtgt tagcataggc
      301 tcatccgatc gtaaccattc agccactgtt aacttgctcg gagaggatat ccttgtggag
      361 cgaatcggga ccgacggaga tatgaataga gcggtcgagc tcatcagcga actcgacgga
      421 aaagttgatg ctttcggaat gggcggcatc gatctttaca tggcgggggt cggcaagaga
      481 tacgtctttc gggatgcaaa aaggatttcc caggcagcaa agaagacgcc gcttgtggac
      541 ggcacaatcc tcaagagcag tttggagtat aacgcagtga agtatgctgc cgaggttgaa
      601 aagctaccca tggccggcaa gaatgtattc gtggtgtgtg ttatcgatcg gataggcatg
      661 ggtcgggcat ttgaagagct cgggtgcaac atggtgtacg gagacttgat gttcagcacc
      721 ggcatgcata ttccgctcta ttcacttaat tccatatata agttggcgcc tatattcatg
      781 ccaataatga ctcaactccc gtttagttgg ctctatccaa cgggcaagga acagaatgcc
      841 agtcgcaagc ggccaaagga caagaaaccc aagtttcaga aatactacaa tgacgctcat
      901 gtcattgcag gcgacttcca ttacatatac aagtatttgc ctgaggatat cagcggaaag
      961 atagtcgtta cgaatacggt gacttcgaag gacgttgatg agctagggcg gcgaggagct
     1021 agcgctctgg tgaccacgac gccagagatc aatgggcgct cattcggggt caacgttatc
     1081 gaggcaatac tcgttgcgtt gatcggcaaa cctgtcgagg atattagtga cgaggatttt
     1141 acggcgatgg tacaatgtct cgagataaag cctcgtattg tgaggtattg atgagggcga
     1201 gatggcagtt ttgccattag atgtgcgaac ctatacatgt gtggcagacc ctggcttctt
     1261 gacaacctgg ggtctgcttg ccataatatt ggcgtgactt ttggcgggag cgccatcttt
     1321 ttttactgca gcgatttatg gatctttcgg gtttgaatct gtccgacggg gcgcgaatac
     1381 aatggttatc atcctagatg gtggctagga attgacgttt gaggggagct tggtatagat
     1441 gtctgacaag gaagtgatcc tcactcccga aggcctccat aacctcgaaa aagagctcga
     1501 gtatctcagg acgacaaagc gcagggatat agcggcccgg attaagcagg ccatagagtt
     1561 cggagatatc agcgacaact ctgagtacga agatgccaaa aacgagcagg cttttgtgga
     1621 ggggcgcatt gctcagctgg aaaagatgct gaggaatgcc aagataatcg aaggacctga
     1681 tggagatggc gacgcggttt ctgtgggttc caccgtagtc ttgaaggatt tgaaccatgg
     1741 tgatgtagaa cagtatacga tagtcggttc agccgaggca aatcctagca gaaatcgtat
     1801 ttccaacgag tcgcctgttg gtcgtgcagt gctaggaaag actgtaggca gtattgtgga
     1861 ggtaaatgtc ccggtgggaa caatcagata cgaagtagtt cagattgcaa ggtgagtgaa
     1921 gataatggac gaacttggag gacaatttga aggcgctgaa ctagggaatg aacttgccca
     1981 taggaaagcg aaggttgagg agtggcgctc acgcgggatt gacccgtatg gctccaagtt
     2041 tgtcaggacg cattgcgctg ctgagattgt cgaggacttt tcagagctgg agggcgaaga
     2101 ggtatcgatc gctgggcgga ttatggcctt gaggggccat ggaaaggctt cattccttga
     2161 tattgtcgat gcatccggtc gcatacagct ttatgccaag cacgatgctt taggggatga
     2221 gctgtacgag ttgcttggaa tgatggacat cggcgacatc attggagtta cagggactgt
     2281 gttccgtacc agacgcgggg agatcagtgt agccatatct tcgttccgca tactttctaa
     2341 ggcgctacgg ccgttgcctg aaaaatggca tgggcttcgc gatgtggata cccgttatcg
     2401 ccagcgttat cttgacctca tggtcaatcc ggaagtgcag aggacttttg ttttgcggac
     2461 taaaataata tcggcgatcc gcgaatacct cgattccaga ggcttcctag aggttgagac
     2521 accgtgcatg catgcgatcg ccggcggtgc agcagctcga ccgttcatta cgcatcataa
     2581 cgcgcttgat atggaattat acttacgtat tgctacggag ctgcatctga agcgtttgat
     2641 agtaggcggg tttgaaaagg tgtacgagct tggccggata ttccggaatg agggcatctc
     2701 cacaaagcat aatccggaat tcacgtcgat cgaagtatat gaagcttatg cagattacta
     2761 cgatatgatg gatcttacag agggtcttgt ggcgtatgtg gccgagacag tgttgggcac
     2821 tacggaagtg gagtatcaag gagataagat cgattttgcg cccccttgga cacggattac
     2881 catggctgag gcagtcctaa aggagacagg cgtcgatttc ctcaacatcg aacagacgga
     2941 ggcggtagcg gcctccagga agttagggct gaaagtgtct gatgatgcta cttgcggctt
     3001 ctgcatgagt gaagcgtatg aggagttcgt agagagccgt ctggtgcaac ccactatagt
     3061 catggattat ccgatagaag tgtcgccgct tgccaaaaga cgggctgaca accccaatct
     3121 cacctacaga tttgaagtgg tatgtgccgg acgcgaattg gctaatgcct tcacagaact
     3181 gaacgatcca gaggatcagc tgcagcggtt cagcgctcaa gttgcccagc gcgagaaggg
     3241 cgatgatgaa gcgcacatga tggatgagga tttcgtgacg gcactcgagt atgggatgcc
     3301 tcctacgggg gggatggggc ttggggttga caggcttgta atgctcctca ccgattcgcc
     3361 atccatacga gatgtgatac tcttcccgtt gatgcgtccg aaagaataag gtggaggcga
     3421 agcgtcttgc aggtgcttgt atattctgat tatgatgaga tgagcgcggc ggccgcgaga
     3481 atgatagcgt ctaagatcgg cgagctcccc aagccggtgc tggggcttgc aactgggtcc
     3541 acgccaatcg gcatgtatgc tgagctggtg cggcttcata atgaggaagg tctggacttt
     3601 tccaacgtca ccacgttcaa ccttgacgaa tattgcggag tcgacgctga ggatcccaga
     3661 agctatcatt ggttcatgaa agagcatctt ttctcccatg tgaacttgga tcctgaaaaa
     3721 gcgcatgtgc ccgcgggaaa cagtgcagat cctgacggcg aatgcgcaag gtatgaaagg
     3781 ctgatcaaag aggccggagg cattgacttg caggttttgg ggatcggaat gaatgggcat
     3841 atcgggttca acgaacctgg ctcggatccg aactcgagga cccgcgtggt gcggctggct
     3901 gagcagacaa tagaggttaa cagagccaag ggcggactga atgccttgcc aggttatgcg
     3961 atatccatgg gtatcggaac aataatggaa gcgcgccaga tattgcttct ggcaaatggc
     4021 gcaggcaagg cggagattat tcggcgtgcg cttaccgggc cggttacgcc cgatgtgccg
     4081 gcttccctgc tccagacgca tcctaacgtt gtgtgtatgc ttgacaagcc ggcagcgtca
     4141 ttgctagatc tttcttgacg caggtatgtg tgtatggtag gattataagc gcagcaagtt
     4201 tggatgggcc cgtagctcag ctgggagagc gctagaatcg cactctagag gtcgcgcgtt
     4261 cgatccgcgt cgggtccacc agtttaatat gtgggtttcc acttatccgg tcgtgacatt
     4321 cacgaccggg ctttttttat gctcgcatct gactgttatt cggtggcgtg catcctgacg
     4381 aaaccatgta ttttcaaggt tggacaagac ttggtgctgc tatagccgtt ggttttgccg
     4441 gtgtcgtcct ttgacccggc aagcgtcgag cgtacgaagt agccacgaaa gtatgggttg
     4501 tgtactgatg tgacatcgta taaaatggaa caggtataat cctttggaag gagggctcac
     4561 ggttggcgta cgagatcaac gataactgca ttgcttgcgg tgcatgcaaa gaggaatgtc
     4621 ctacggaggc catcagtgag ggcgatattt acgcaataga cccagagaag tgcattgact
     4681 gcggtacatg caacgaggtg tgccctgtag gagcgccgaa tccagcttga ggaaaaggct
     4741 atattgaatt ggcaatccgg ttgtggactt ctcgatgtac catgtacgtt ggggggtcca
     4801 tgattcatat tatgacaatc cacgggcaga aacagatgaa aacccgacca ggctgcttcc
     4861 tcagtggtat tctctgaagt ctgcggatgt gggtttgccg tcatcagccg cgccctcaaa
     4921 ccccgcgggg gcggcgcgtg ttgtgcgcgc attagcagtg tagtagaata aatgatggat
     4981 ttcggtggtt ggaggggtgt agtatggcaa tcggggagct tgtgaattct gtcgatagat
     5041 ctaagttgga gtttgttgag aagctccggg gcggagtgat catggatgtc accacgccag
     5101 agcaggctaa gattgctgaa gacgccgggg ccgttgcagt catggctctt gagagggtgc
     5161 ctgcggatat tcgcgctcag ggcggcgtag cgagaatggc cgatccggcc ctgatagagc
     5221 agatcatgga ttcagtttcc atccctgtaa tggcgaaggt gcggataggg cattttgtgg
     5281 aggctcagat actagaagcg ataggcgtac attgcataga tgagagcgaa gtcctgactc
     5341 cggctgatga gagatatcat gttgacaagc acgctttcac gacacctttc gtgtgcggtt
     5401 gccgcaatct cggcgaggcg ctccgcagga tcggtgaagg cgcttcaatg atgcggacaa
     5461 aaggcgaggc aggaacagga gacatagtag aggcggtccg ccacataagg gcggttaatg
     5521 aggatattcg gcggctcaag aatatgcctt gcgaagagat aatggccatg gccaaagaga
     5581 ttggagcccc atacgagctg ctttgccaag tggcccggga tggcaagctc ccagtcgtga
     5641 atttcgccgc cggtggcgtg gcaactcctg cagatgccgc tctaatgatg caattgggag
     5701 ccgacggcgt ttttgtgggt tcaggcatct tcaagtcgga tgatcc
//
LOCUS       DAOSAI010000123         5745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8879_length_5745_cov_3.982601, whole genome shotgun sequence.
ACCESSION   DAOSAI010000123 DAOSAI010000000
VERSION     DAOSAI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8879_length_5745_cov_3.982601"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLM83_05410"
     CDS             <1..647
                     /locus_tag="PLM83_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOK70878.1"
                     /translation="IDDRYYVTWCNGYHGPTIGVAYTYNFKEFFQLENAFLPYNRNGV
                     LFPRKIGGKYMMLSRPSDRGHTPFGDIFCSQSPDMIHWGCHRHVMSPRGSWQSTKIGP
                     GPVPIETSEGWLLIYHGVLTSCNGFVYHMGAALLDIDRPWKVLCRTEPYILSPQEIYE
                     CVGDVPNVVFPCAALADSATGRIAIYYGAADTVTCLAYAYVDELVDFVKSNSTL"
     gene            complement(1053..2633)
                     /gene="pckA"
                     /locus_tag="PLM83_05415"
     CDS             complement(1053..2633)
                     /gene="pckA"
                     /locus_tag="PLM83_05415"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOK70879.1"
                     /translation="MTDIISELRSIGLVNTGLVHHNLSAPQLAEEALARGEGILTSTG
                     ALRVVTGKYTGRSPDDKFIVDDTETHSTVDWGPINKPMSEESFIRLYARLAAYLQRRE
                     LFIFDGYAGTDPLYELPIRVVNEFAWQNLFAHQLFVRPTDDQLKSHKPEFTVIAAPGF
                     KANPEMDGTNSEAFVIINFSKRLVIIGGTSYAGEMKKSIFSVMNYVMPKADVLPMHCS
                     ANIGPDGRSALFFGLSGTGKTTLSADPDRQLIGDDEHCWSKHGVFNIEGGCYAKCINL
                     SREHEPQIWDAIRFGTVLENVVVDPKTRIPDYDDDTITENTRAAYPIYHMPSAAEPSM
                     GDHPSTIIFLTADAFGVMPLISKLDANQAMYYFLSGYTSKLAGTERGIVEPEATFSSC
                     FGAPFMPLPPMVYAAMLGDKLTQNNSSVYMVNTGWIGGQYGVGRRVSLKHTRAMVSAA
                     LSGALDHVSYRAHPIFRLMMPKECPGVPSDILDPKSLWQDAEAYDQAALGLAARFNKN
                     FGKFHDVSPEIASAGPKA"
     gene            complement(2879..3451)
                     /locus_tag="PLM83_05420"
     CDS             complement(2879..3451)
                     /locus_tag="PLM83_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HOK70880.1"
                     /translation="MWAYEKKLEYPVKVSGPNPKLAKVVVTQYGGPDGELAASLRYLT
                     QRYTMPTSQAKGVLTDIGTEELAHMEIVAALVYNLLKGAPLSEIKAAGMDGYYADHDR
                     SVYFVNGDGVPWVAAYIQSKGDPIADLHDNMAAEEKARATYEHLINLSDDPQVTDVLR
                     FLREREVVHFQRFGETLNIVQDFLDRRKVW"
     gene            complement(3486..3743)
                     /locus_tag="PLM83_05425"
     CDS             complement(3486..3743)
                     /locus_tag="PLM83_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HOK70881.1"
                     /translation="MRTPINREELLLRIQALEFAALDLNLYLNTHPGDARALADFNQI
                     SAALAQTRTAYEQAFGPLMNYGHSLSCGRWAWIDEPWPWEA"
     gene            complement(3743..3931)
                     /locus_tag="PLM83_05430"
     CDS             complement(3743..3931)
                     /locus_tag="PLM83_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HOK70882.1"
                     /translation="MSQDSLEWAQAMKLARAYVRPQRYTQRFDPLEGLKNGTIFPELV
                     SPYTPPAETSRSHEEALG"
     gene            4110..4994
                     /locus_tag="PLM83_05435"
     CDS             4110..4994
                     /locus_tag="PLM83_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4438 domain-containing protein"
                     /protein_id="HOK70883.1"
                     /translation="MIRTNRDRLVMVSVQGAVAPHVRKQAFRIDADGVPFVLPGVGGI
                     TYNVRVGDPAFGWAGDHVEPGVSTAADYDKRGDEKNRAYNILACIGNKARVISGDAKG
                     AEGVVTGHHGGIEHVLVDFDDDAMEKLAIDDKILIRAYGQGLELLDYPEIRVFNVDPD
                     LLDLIGLEQDGTRIRVGVAAVVPAVLMGSGIGSADVASGDYDITTTDKAEISRLGIDK
                     LRFGDIVALQDCDNLYGRSYRQGAVSIGVVVHSDCRVAGHGPGVATIMTSAKPTIEPV
                     IDDSANIGRYLNIGRYRK"
     gene            5090..>5745
                     /locus_tag="PLM83_05440"
     CDS             5090..>5745
                     /locus_tag="PLM83_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70884.1"
                     /translation="MGVSAVGKLPKVELHRHLEGAVRPATALDLFRKNCGMFLDAAVD
                     DILPKMQITEADSSLQDFLAKFDFFMPCLRSAEDITRITVEAIQDCAAEGIIYAELRF
                     SPVFIKGLTGLSYVDAVEAVLEGARIAADCGPIVNFIVIIPQPAGEKAGWDSVRIARD
                     YMADGITGVDIADDTRVLGLEEYVRPMTWARANGLGVTVHAGESEGPESVRTAVERLG
                     A"
BASE COUNT         1254 a         1380 c         1674 g         1437 t
ORIGIN      
        1 ggatagatga tcggtattat gtcacatggt gcaatggata ccatgggccg acgataggcg
       61 tggcatacac gtacaacttc aaggagtttt tccagcttga gaacgcattt ctgccgtaca
      121 acaggaatgg agtgctattt ccgcgcaaga taggcggcaa gtacatgatg ttgagccgac
      181 cgagtgatcg aggtcatacg ccgttcggcg acatattctg cagccagagc cctgatatga
      241 ttcactgggg ttgtcaccgc cacgtgatgt cgcctagggg atcgtggcag agcacgaaga
      301 tcggccctgg acctgtgcct attgagacca gtgagggatg gctcctcatc tatcatggag
      361 tcctcacatc gtgcaatggg tttgtgtacc acatgggcgc agccctcctc gacatagatc
      421 gaccttggaa ggttctctgc aggacagaac cttatatact atctccacag gagatatatg
      481 agtgcgtagg ggacgtgccg aatgttgttt ttccgtgtgc agctcttgca gattctgcca
      541 ctgggcgtat agcaatctac tacggcgctg ccgatacagt gacatgcttg gcatatgcct
      601 atgtagatga attggtggat ttcgtgaaga gcaactcaac actatagcta gcctgtgtgg
      661 ccctctcggc tttggccaat cgtagggagg ctgaacttca acccctgcct gtcggctttt
      721 gactcacagg ctaggaaacg gctggaaggc ggctcgaggg gttgcaagcc gtttgggcct
      781 ggattctccg gatgagggca cgtagttcgt gcagaatggt ccaatatgaa ttctgaagca
      841 tgctgttgtg gaagttcaag aaggtcatgt gatgatgata atgacagcgg ttgcttcagc
      901 ataagagttt cagactgagc gtggagaatt caaaaacaga aattgcagcc gcattttgtt
      961 ccgcccccgc gaaaagaacg cctgacagct cggacgctgc aaatcctccg actgatcagg
     1021 cgctctttgt atggctgcac gaacctctca ggttacgctt ttggacccgc agacgctatt
     1081 tccggcgata catcatggaa tttcccaaag ttcttgttaa accgcgccgc caatcctaat
     1141 gctgcttggt cataggcttc cgcatcctgc cataggctct tggggtcgag tatgtctgat
     1201 ggcactccag gacattcctt gggcatcatc agtctgaaga ttggatgagc tctataggat
     1261 acgtggtcca aggctccgct gagtgcggcc gaaaccattg ccctagtatg tttgaggctt
     1321 acgcgcctgc ctacgccgta ttggccgcct atccaaccgg tgtttaccat gtacacgctc
     1381 gagttgttct gggtgagctt atcgccgagc attgcggcat acaccatggg tggaaggggc
     1441 atgaacggag ccccgaaaca tgaggagaac gtggcttccg gttccacaat gccgcgctcg
     1501 gtgcctgcaa gcttgcttgt gtaacccgac aggaagtaat acatggcctg gttggcgtcg
     1561 agcttcgaga ttaggggcat gacgccgaaa gcatctgccg tgaggaagat tatcgttgaa
     1621 ggatgatctc ccatgcttgg ctcggctgcg ctgggcatgt ggtatatggg atatgctgcg
     1681 cgggtgtttt cagttatcgt gtcgtcgtcg tagtcgggaa tgcgagtctt ggggtcaaca
     1741 acgacgtttt ccaggacagt cccaaaccgt attgcgtccc atatctgcgg ctcatgctcc
     1801 cgcgaaaggt tgatgcactt ggcgtagcag ccgccttcta tgttgaagac tccgtgtttg
     1861 ctccaacaat gctcgtcgtc gcctatcaac tgcctgtcag gatccgctga cagggtagtt
     1921 tttcccgtac ctgaaaggcc gaagaagagg gcgctccgtc cgtcgggccc aatgttggca
     1981 gagcagtgca ttgggagtac gtccgccttt ggcattacgt agttcatgac agaaaagatg
     2041 gatttcttca tttcgcccgc ataactcgtg cctccaatga tcactagccg cttggagaaa
     2101 ttgatgatca cgaaagcttc tgagtttgtc ccgtccattt cagggttggc cttgaatccc
     2161 ggagccgcta ttaccgtgaa ctcgggcttg tgtgatttga gttgatcatc tgtcgggcgt
     2221 acaaaaagtt gatgcgcaaa gaggttttgc catgcgaact cgttgaccac gcgtattggc
     2281 agttcgtaaa ggggatcggt tcctgcatag ccgtcaaata tgaagagctc gcgtctttgc
     2341 aagtaggcgg cgagacgtgc gtatagcctg atgaagctct cttctgacat cggtttgttg
     2401 atcggtcccc agtcgacggt ggagtgggtt tccgtatcgt caacgatgaa cttgtcgtca
     2461 ggtgaacgcc ctgtatactt gcctgtaaca acgcgcagtg ctccagtgga cgtcagtata
     2521 ccttcaccgc gagcgagtgc ttcttcggca agctgtggcg cggagagatt atgatgaact
     2581 agtcctgtat tgaccagacc gatcgatcga agttctgaga tgatgtccgt cactatgctc
     2641 acttcctgtt gctaaagttg ttcatagatt accacaccga gtcgtctgtc agaagtactc
     2701 gaatacgcgt cggccttctg cggttggtcc cattgaggga cggtgctcgc cgtagagggt
     2761 ttggagtggg ccgcctatga agaggccgcc ctcgacgtgg ggcggcacaa cataggatat
     2821 cgatcttttg cagcgcgcct cttcactgac agtcatgcca tgcgtatgtt gctcaaaact
     2881 accagacttt gcggcggtcg aggaaatcct ggacgatgtt gagagtctct ccgaatcttt
     2941 gaaaatggac tacctcgcgt tcacgcagga accgcaagac atctgttact tgtggatcgt
     3001 ctgacagatt aatcaagtgt tcataggttg ccctggcttt ttcttcagct gccatattat
     3061 catgcagatc agctatgggg tcgcccttgg attggatgta agcggctacc catgggactc
     3121 cgtctccatt gacgaagtag actgaacggt catggtctgc ataataacca tccatcccgg
     3181 ctgctttgat ctcgctgagt ggagcgccct tcaaaagatt atacactagc gctgcgacta
     3241 tttccatatg cgccaactct tcagtgccaa tatctgtaag aacgcccttg gcctgagaag
     3301 tgggcattgt gtatcgttgt gttagatagc gcagggaagc ggccagctct ccatcagggc
     3361 cgccatactg ggtgaccaca accttggcta gcttggggtt cggcccgctc accttgacgg
     3421 ggtattccag tttcttctca tatgcccaca ttcctatatc ctccccgttt catatctagt
     3481 cttgcctatg cttcccacgg ccacggttcg tcgatccatg cccatcggcc gcaagatagc
     3541 gaatgtccgt agttcataag cggaccgaaa gcctgctcgt aagccgtgcg cgtttgggca
     3601 agagctgcgg atatctgatt gaagtccgct agtgctctgg catctccagg atgggtgttg
     3661 aggtaaagat tgagatctag tgcggcgaat tccagagctt gaatacgcag aaggagttct
     3721 tcgcggttga tcggggtccg cattatccta acgcctcctc atggctgcgc gatgtctcgg
     3781 caggaggcgt atagggcgac actagttcgg ggaagatggt gccattcttg aggccttcta
     3841 acgggtcgaa gcgttgtgtg tatctttgag ggcgcacata ggcgcgagca agtttcatcg
     3901 cttgggccca ttctagacta tcttggctca ttttactttc ctccctgatt gcgtatgcga
     3961 atgctcagtc catattattc aggagctttt ccgagggtat gatgcgacgc gcacatttga
     4021 tccagttcgt gctggtggaa tcaatgcgtg aaggaatcgc cgcatacaag gtgaatcatc
     4081 tcgttagatg ctacaggagg gtggtagaga tgattaggac taacagagac aggcttgtaa
     4141 tggtttcggt tcagggcgct gtggcgcccc atgtgaggaa acaggccttc cgcattgatg
     4201 ccgatggggt tccatttgtt cttcctggag ttggaggaat cacgtacaat gtcagggtgg
     4261 gtgatcctgc attcggttgg gcaggtgacc atgttgaacc aggtgtctca acagctgcgg
     4321 attatgacaa gcggggagat gaaaagaacc gggcttataa tatccttgca tgcataggga
     4381 ataaggctcg cgtgataagc ggggacgcta agggggcgga aggggttgta accggccatc
     4441 acggaggcat cgaacacgtg ttggtggact ttgatgatga tgccatggag aaactggcta
     4501 tagatgacaa gatcctgatc cgagcttacg ggcagggcct tgagttgctc gattatccgg
     4561 aaatcagagt attcaatgta gatcctgatc tgctagatct gattggcctt gagcaagatg
     4621 gcacccgcat ccgtgtggga gttgcggctg tagtgccggc ggtgctgatg ggttcgggga
     4681 taggatctgc ggacgtggcg tccggcgact acgacattac taccaccgac aaggccgaga
     4741 tctcgcggct tggtattgat aagctgcgct ttggcgatat cgtagctctg caggattgtg
     4801 ataacctcta cggacggagc taccggcaag gcgccgtgag tattggtgtg gtggtacact
     4861 ccgattgtcg cgtggcaggg cacggccctg gtgtggccac cattatgacg tcggctaagc
     4921 ccacaattga accggtgata gatgattcgg ccaatatcgg ccgttatctg aatattggaa
     4981 gatacagaaa ataacgttgc tcgggagaga cggcccggca tgtcaactcg gaaatcggga
     5041 gtgagcgcgc cgagccgcta ctatttattg gctggggggg aaggtaaagg tgggagtttc
     5101 agctgtagga aagcttccca aggtagaact acacagacat cttgagggcg ctgtgcggcc
     5161 ggccactgct ctagatcttt tccgtaagaa ctgcgggatg tttctggacg cggcagtcga
     5221 cgatatcctg cccaagatgc agataactga ggccgacagc tctcttcagg attttttggc
     5281 caaattcgac ttcttcatgc cgtgccttcg ttccgcagag gacataaccc gaataactgt
     5341 cgaggccata caggactgcg ctgctgaagg aataatctac gcagaactgc gattctcgcc
     5401 cgtcttcatc aaggggctga ccggactttc atatgtggat gcggtagagg ctgttctgga
     5461 gggagcgcgc attgcggcgg attgtggccc gattgtgaac ttcattgtga taattcctca
     5521 gcctgccggg gagaaagctg gctgggatag tgtcaggatt gcccgtgact acatggctga
     5581 tggaattacc ggcgtggata ttgcagatga cacaagagtg cttggccttg aggagtatgt
     5641 gcggcctatg acatgggcgc gagctaacgg actgggcgtc acggtccatg caggagaatc
     5701 agaagggccc gagtcagtgc gaacggctgt ggagagatta ggggc
//
LOCUS       DAOSAI010000124         5724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8916_length_5724_cov_3.438878, whole genome shotgun sequence.
ACCESSION   DAOSAI010000124 DAOSAI010000000
VERSION     DAOSAI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8916_length_5724_cov_3.438878"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLM83_05445"
     CDS             <1..416
                     /locus_tag="PLM83_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70885.1"
                     /translation="ALFVVLLIIGREYIGLGTFAGSFVTGAMADVFSAAMRSPTAPWQ
                     SWPILIASIAVISSGVAIYLSAELGDGPLEGVMMLISDRLGAPIAWVKIAMDIAATVA
                     GIAMGAVPGVGTIASVLLTGPIIQTMLQVIKKSMA"
     gene            complement(456..1262)
                     /locus_tag="PLM83_05450"
     CDS             complement(456..1262)
                     /locus_tag="PLM83_05450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK70886.1"
                     /translation="MIALRIGVNQICTPNGDIGRFVRGTCGHIVDRNDDGSETTVGII
                     AGLWFQIDDWIIVGKPCDYRDMFSQFSPEVSLAGDLLVPEPPEIRRDVTDSDRHDIIL
                     LECVFVEKPYRNCGIGTAALEGFLRYARSRAGVVAIEPFPMEQGDDGVIRAALKSSVP
                     VEHKPAGPGSECELLPISDGGNCSTLSPRFLRRLGFEPIPEECCHHFAVDPSACSPLA
                     RQRAADGNPCPGLMTVDDIAVEVVYSPVQLPVPYEGMKAVVTIDAPDVAW"
     gene            complement(1438..>3353)
                     /locus_tag="PLM83_05455"
     CDS             complement(1438..>3353)
                     /locus_tag="PLM83_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOK70887.1"
                     /translation="LETAETLQRVDDIYRPYRPKRRTRASMARERGLGPLAEWLLLRP
                     ETGDPLERAAEYVNLEAGVASAEEALAGARDIVAEILADDPEVRAWARQWTAGHGEIS
                     TEATDSEARTTYEQYYDYCEPVNRIPPHRVLAINRGEREKVLRVRITLEAEPIVAYLK
                     RRLMREGPKSSPADAELALVAKDAYDRLLAPAVERDVRNALTEEAEQQAIRVFGANLK
                     ALLLTPPIRGKVVLGLDPAYRTGCKIAVVDATGKLLETTVVYPTPPQKRIEEAEQVLL
                     KIIDEHGVDLIAIGNGTASREAEAFVAGLIPKAERSISYVVVNEAGASVYSASKVARE
                     EFPDLDVSERSAVSIARRLQDPLAELVKIEPRSIGVGQYQHDVSGKELSTTLNGVVES
                     AVNRVGVDLNTASVPLLSYVAGISPSVAANIVAKRESEGPFGERKELLSVSRLGPKTF
                     EQCAGFLRVPGGRYPLDNTAIHPESYPVAEHLLAELGFAVDDVAERDRTKLCQRLSAL
                     DAQDWAERLDVGLPTLEDIIEALQRPGRDPRDEFPQPILRKDVLTMDDLRPGMMLEGT
                     VRNVVDFGAFVDIGVQQDGLVHISELSNRYVRHPLDVVSIGDIVKVRVLSVDIERGRI
                     ALSMRDIGEEKLG"
     assembly_gap    3354..3363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3364..3616)
                     /locus_tag="PLM83_05460"
                     /pseudo
     CDS             complement(3364..3616)
                     /locus_tag="PLM83_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006040783.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
     gene            complement(4007..5260)
                     /locus_tag="PLM83_05465"
     CDS             complement(4007..5260)
                     /locus_tag="PLM83_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOK70888.1"
                     /translation="MTLRKQHCRFLLLLLLAIIIAWPQAALATSAAGSEQQEQMTVVV
                     CVIDGCPNLSLGCLPETAYLRQLCADERCQYKSIEAVFPSSTAAGHAAIFTGTYPERN
                     GVTGKEYLDDDGELLRFKDPAVLEEPTLFHVARSAGLGTAMISAKANLCKMLSEATDF
                     AIWPESVPDWVIETVGPPPDESTQYEDFAGWHIQLDRWVLRTVEAYLEQNDMPVFVGI
                     NVGSVDKCGHRFGPLPAAETAECLCSISEGLEELAATLEATRPGRWSLIITADHGMTR
                     VDTGIAARDIVGDITDEDVPITLDGGVLYVWAEDNRGKIADALRQTEGVAEVIEVEDV
                     ARRAELHIEHPRTPPLIAVTDRGCMFIEALLFMDYTLGSHGTTLDTDVDVPLIVVGRN
                     ADSVDIENVSSITDIASIARELLGI"
     gene            complement(5257..>5724)
                     /locus_tag="PLM83_05470"
     CDS             complement(5257..>5724)
                     /locus_tag="PLM83_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70889.1"
                     /translation="LGSMCFASPAKPSGKVVLTVKGPIANTNAKGACEFDLEMLEGLG
                     LTEYQVKDPWLGKKTYAGVNIAKILGYVGAPKNVSEVVVIAKDGKEVIISAADVAKYE
                     IMVATKDGGKAIGSGLGGPVKLVFPYDDNPGLDKQYPKESWNWFVTTIEVRTK"
BASE COUNT         1244 a         1774 c         1363 g         1333 t
ORIGIN      
        1 tagccctctt tgttgtattg ctcatcatcg gcagagaata tataggattg ggcacatttg
       61 cggggtcctt cgtcactgga gccatggctg atgtgtttag cgccgcaatg cgcagcccta
      121 cagccccttg gcagtcgtgg cccatactga tagcatcgat agcggttatc agctcgggag
      181 tggcaatcta cctctctgca gagctgggag acggaccttt ggaaggagtg atgatgctta
      241 tcagcgaccg cctcggagca ccaatcgcct gggtcaagat agcaatggac attgccgcaa
      301 cagttgcagg tattgcgatg ggggccgtgc ccggagtcgg aacgatcgct tccgtcctct
      361 taaccggccc catcatacag acaatgcttc aggtaatcaa gaagtccatg gcttaatggc
      421 caattgagtc ggagcgtgcc tttttccttc gactgctacc acgctacatc tggcgcgtct
      481 atcgtcacaa cagccttcat gccctcatac ggaactggta actgaaccgg actgtagacc
      541 acttcgactg cgatgtcatc gacagtcata aggcccggac atgggttccc atctgcagcc
      601 ctctgcctcg ccaaaggcga gcatgcgctt ggatcaacag cgaaatgatg acaacattcc
      661 tcaggaatcg gctcaaaccc gagtctgcgc agaaaacggg gcgaaagagt gctgcagttg
      721 ccaccgtctg atatgggcag caactcgcac tcagatccag gccccgccgg cttgtgctca
      781 acaggcactg aacttttcaa agctgctcgg atgacgccat catccccctg ttccatggga
      841 aacggttcaa tggccaccac cccggcgcgt gatcgcgcat atctcagaaa gccctccaaa
      901 gccgcggttc caataccaca gttgcgataa ggcttttcta caaaaacgca ttccaagaga
      961 atgatatcgt gcctgtccga atcagtgaca tcccttcgta tctcaggagg ctccggcaca
     1021 agcaagtcgc ctgccaggga gacttccggg ctgaactggc tgaacatatc ccggtaatca
     1081 catggtttgc ccacgatgat ccagtcatcg atctgaaacc ataggcctgc tattatcccc
     1141 acagtcgttt ccgatccatc atcgttcctg tcaacaatgt gtccgcatgt gcctctcacg
     1201 aacctgccga tatcgccgtt gggtgtacaa atttggttga cgccgattct aagtgcaatc
     1261 atcacgacag cctcctcccg tagattactg tgacgagtag gccaccgcat catagcacac
     1321 aggtcggcac aatcatgtca ccgggctagt ccgacagctc acgactttct tcaaacaagg
     1381 actaggcacg tggcgccacc gccacaacca gtttccagcc gattgttgca acgtcgttca
     1441 acctagcttt tcctctccta tgtccctcat ggataaggct attcggcccc gctctatatc
     1501 gaccgacagc acgcgaacct tcacgatatc ccctatactc accacatcca gcggatgcct
     1561 gacgtaccta ttggaaagct cggatatatg aacgagccca tcctgctgta cacctatatc
     1621 tacgaacgcg ccgaagtcca ccacatttcg cacagtgcct tcgagcatca tgcccggacg
     1681 caggtcatcc atggtcagca cgtccttgcg gagtattggc tgtggaaact catcccgcgg
     1741 gtcgcgacca ggccgctgaa gcgcttcaat gatatcttcc agcgtaggca ggccaacatc
     1801 gagccgctca gcccaatctt gagcatccaa ggcgctcagc ctttgacaaa gcttagtccg
     1861 atcgcgttcc gcaacgtcgt ccacggcaaa ccccagttct gccaatagat gttcggccac
     1921 agggtaagat tcggggtgga tcgcggtgtt gtccaacgga tacctgcccc ccggcaccct
     1981 caagaagccg gcgcactgct cgaaggtttt ggggcccaac cgggagactg aaagcagttc
     2041 ttttctttcg ccaaaaggcc cttcggactc tcgttttgca acaatattgg cggctactga
     2101 tgggctaatt cccgcaacat acgacagcag aggaactgac gccgtattca ggtcgacacc
     2161 cacgcggttt acagcagact caaccactcc attgagcgta gtgctcagct ccttgccgga
     2221 aacgtcgtgc tgatactggc cgacgccgat ggatcgcggc tctatcttca ccagctcggc
     2281 caggggatcc tgaagccgac gcgctatgga gacagcgctg cgctccgaaa catccagatc
     2341 agggaattct tcgcgcgcaa ccttggacgc ggagtaaact gatgctcccg cctcatttac
     2401 aaccacatac gaaattgacc gctccgcctt gggaatcaag ccagccacaa acgcttccgc
     2461 ctcgcgggat gctgtcccat tgccaatggc aatcaggtcc accccgtgct cgtcaatgat
     2521 tttgagcaag acttgctcgg cctcctcgat ccttttctga ggaggtgtag gatagactac
     2581 agtggtctca agcagcttac ccgttgcatc taccaccgct atcttgcacc ctgtgcgata
     2641 cgcgggatcg aggccgagca cgaccttgcc acggatcggc ggcgtcagga gtaaagcctt
     2701 caggttggct ccgaataccc tgatagcctg ctgctctgct tcttccgtca gcgcattacg
     2761 cacgtcccgc tcaactgcag gggccaacag acggtcgtag gcgtcttttg ccacgagggc
     2821 caattcggca tctgccggac tcgacttcgg cccttctcgc atcaacctcc gcttcaggta
     2881 tgccactata ggctctgctt caagcgtgat acgaacgcgc aagaccttct ccctctcccc
     2941 cctgtttatc gccaacactc gatggggagg aattctattc acaggctcgc agtaatcgta
     3001 atactgctca taggtagtgc gcgcctcaga gtcggttgct tctgttgata tttcgccatg
     3061 gcctgccgtc cattggcgag cccatgcccg cacttcagga tcatctgcca gaatttcggc
     3121 tactatgtcg cgcgcacctg ccaaagcctc ttccgcgctg gcaacacccg cttcaagatt
     3181 gacgtattcc gccgcccgct cgagaggatc accagtttca ggccgcaaga gcaaccattc
     3241 tgccaatggc cccaaacccc gctcacgcgc catgctcgcc cttgttctgc gcttgggacg
     3301 gtatgggcgg tatatatcat caacacgttg gagggtttca gccgtctcca acannnnnnn
     3361 nnnctggcgt cagcgccccc tgatcgtcta tgagcctcat cacctcgagc ttgcgcgtct
     3421 caagattccg caggtaatca agcctctcat ctatagagcg aatggcttcc tcatcgagtc
     3481 ccccggtctg ttctttcctg tagcgggcta agaaggggat ggtgtttccc tcatccagga
     3541 gacgtatgac gctatccacc ttggatcttg cgattccaag ctccccgctg atcttggttg
     3601 caatccccgc ccacatctgc gcagcgattt ctgcctcatt cggagtccct tgtccttcca
     3661 tgtcctctgc ggtcgcacgt agtccatctt tctcttgttc ttcagttgcc aaagactcga
     3721 cttccatatc ttaacttgcc ccctgtaata tccaactcaa tgcgtgcgat agtattccgc
     3781 aatcgccgat ttaatcttag catccttttt gagtcagtgt cgaacggcgc ggccatgcgc
     3841 atacgcacaa catggcgaca tggacctgta acttgccatg acatagcata tcgtcacatg
     3901 cgggacaaag aacggtaaag gcctgccaga atgccttctt ggcaggccct tgccccatgt
     3961 actacgactc aatctgcata cataactaac gctaatcgga taacagtcag attcctaaca
     4021 gctctcttgc gatagaagcg atatccgtta tcgagctcac gttttctatg tccacactat
     4081 cggcgttccg acctactaca atcaaaggga catcaacgtc agtatcgagg gtagtcccgt
     4141 ggcttcccag tgtgtaatcc atgaataaca atgcctctat aaacatgcag ccacggtcag
     4201 tcactgcgat cagcggaggg gtgcgaggat gctctatgtg gagttctgcg cgtctagcca
     4261 cgtcttcaac ctctatcact tcagccacgc cctcagtctg tctcagcgca tccgcgatct
     4321 tccccctgtt atcttcggcc caaacgtata ggactccgcc gtcaagagtt attggcacat
     4381 cttcatcggt gatatcgccg acgatgtccc ttgccgcgat gccggtgtcg accctagtca
     4441 tgccgtggtc tgctgtgatg atcaaactcc accgtcccgg acgtgtcgct tccagtgttg
     4501 cagccaattc ctcaagacct tcgctgatag agcagaggca ctcagctgtt tcggcagcgg
     4561 gcaaaggccc gaacctgtgt ccacacttgt caacgctccc aacgtttatg ccgacaaata
     4621 caggcatgtc attctgctct agataggcct cgaccgtcct caaaacccac cgatctagtt
     4681 ggatatgcca tccggcgaaa tcctcatact gggtggactc atcgggcgga ggacccacag
     4741 tttcgatgac ccaatccggt accgactcgg gccagattgc gaagtcagta gcctccgaga
     4801 gcatcttgca caagttagct tttgcggaga tcattgcggt acccaaccct gcagagcgcg
     4861 ccacatggaa cagcgtcggc tcttccaaaa ccgcaggatc cttgaaccgc aatagctccc
     4921 catcatcgtc tagatactcc ttgcctgtta ccccatttct ctccgggtat gttcccgtga
     4981 aaattgccgc atgcccggcg gctgtcgaag acgggaatac tgcctcaatc gacttatact
     5041 ggcatctctc gtcagcacac agttgcctga ggtacgcggt ttcaggcaag cagcccagtg
     5101 acaagttagg gcacccatct atcacgcaga ctaccaccgt catctgctcc tgctgctctg
     5161 acccggcggc gctggttgcc agcgccgcct gcggccaggc tatgattatg gccaatagca
     5221 gcagtaacag gaacctgcaa tgctgcttgc gcaatgtcat ttcgtcctca cttcaatggt
     5281 cgtgacaaac cagttccagc tctccttcgg atactgcttg tcaaggcctg ggttgtcatc
     5341 atacgggaaa acaagcttga cggggccacc aagccctgat ccgattgctt ttccgccatc
     5401 tttcgtggct accatgatct cgtacttcgc aacgtccgca gcgcttataa tgacctcttt
     5461 gccgtccttt gcgattacca cgacctcact tacgttcttc ggcgctccca cgtagcccaa
     5521 tatcttggcg atattcacgc cagcgtatgt ttttttgcca agccagggat ctttgacctg
     5581 atactcagtg agtcctagtc cctcaagcat ctccaagtca aactcgcatg cccctttagc
     5641 attggtattg gctatagggc ctttcacggt caggaccacc ttgccgctgg gtttcgcggg
     5701 actcgcgaag cacatcgaac ccaa
//
LOCUS       DAOSAI010000125         5717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8933_length_5717_cov_3.788944, whole genome shotgun sequence.
ACCESSION   DAOSAI010000125 DAOSAI010000000
VERSION     DAOSAI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8933_length_5717_cov_3.788944"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1798)
                     /locus_tag="PLM83_05475"
     CDS             complement(167..1798)
                     /locus_tag="PLM83_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOK70890.1"
                     /translation="MDRKHGFNRHLCFIVLCLLLATASTAAVGASPIKVDFYVIVPDR
                     TPTGDIVYIGSEAANQRDAASVAMRHIGNNVWTTTLEIAEGQILDYKYTRGTWETVEK
                     DSTGADIETRSVVALDLGGGQLLVIDYVPLWADELDRADVTPITALQSIAPAYEESDT
                     LEAPIREIVYLGSQDFYDRLPVLYLHEPVKPAKNAILLINDGTGLSHRTLARICAGGP
                     NYRLPSEQMPIVGLANTHSRGSLVTDSAAAGTQLATGYWVNNRTISVDPDGVPLKTIL
                     ELAKELSKSTGIITTDSVTGATPASFGSHAGDRGDDPAISTGIFDNRVDVVMGGGRKH
                     FVPGTDNGARIDGRNLLKELQDEGFTVVDNVDDLKADNNLRAWALLNSGSLTGAASRN
                     YTLGDLTEEAIRRLSQNENGFFLMVEGDQIDGGGHNNDGYRVVTEMLDFETAVQVAVD
                     FASGRDDTLVVVVADHETGGLGITSGSAYGTSAEIGWVHTGHTGSPVAVYSCGPNSVL
                     LGSHMYLANIPKIISLGWGVTDFGPERELTGPGAF"
     gene            2308..3585
                     /locus_tag="PLM83_05480"
     CDS             2308..3585
                     /locus_tag="PLM83_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK70891.1"
                     /translation="MGANSAKDRRFDRVRRWMLLAVIIVSLLGCSSHGLAAKTRLIIW
                     WPQGSTEKAVLQAAIADYAKVNPSVEIVPQWQPDSDDTQKLKIAYVGGNAPDIVRIDH
                     VWVQALGYKRMFTDLVPYGANSVKNMFFDPTWQANTYKDKVFALPFDANVICLMYNNT
                     LLKSTGTTVPTTYDELIAVGKANTRDINNDGKIDKWGYTIPVSAGGSGWLQFQWLTWL
                     WRMGGEVLNEDWTRAVFNSPEGVKSLEMMIDVVKKHKIAPDGAYYEGDFYNGNVAMID
                     MGPWSMSGRDPKVFGFAPLPVLNPRVGEATGLGLYSLAITSSCKNPKAAYDFVKFVTT
                     TPKYSAMWSKGTYLMPSLKTAYSDPLYRTGYWSVFMSQLPKAKMRPGTPAWPEIAGYI
                     SDAIQSVLAEKATAAEALNKAAELSNKALSKVK"
     gene            3588..>5717
                     /locus_tag="PLM83_05485"
     CDS             3588..>5717
                     /locus_tag="PLM83_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOK70892.1"
                     /translation="MATFLRGMVAHGGAIIPAIVENASFAVKMIGEIYVICSQAAPFV
                     KAVSVIVAIAILLGSVSGTCSAAMQMDGVSEVSHRFPCIARPERLYVISKSAMTDAEL
                     LMVSSLQGIVAQTRPEIYIDSGSAYRGWLELIRAEHGIPMQYHDEAEWFVDHYKDIFK
                     ENGYVLADLKSECVNVATTVSGLLGAPIVDQSLLPLMCVRGFEMAIDVRDKDECWAFE
                     NYKDKLNPNILFRQDPLKLQLRDYAIACKGFVFYQPDNDEFVSTVYDWAQEDAPVLGW
                     GPGFEDDHVAKASEHNHMTIPADWAMNLSALAGIGHGSLQQRNHVANIRFEPDVHYVT
                     FVMSDGDNVQWLLGDMAVKPHHYGSAVRGKFPMGFTISPSLIGLAPAAMGWYYDTAKL
                     DYFVAGVSGTGYIYPSKYPRLDSFAFELDQYLAMSDLSIVSILDQCPMGSKEFHDLAQ
                     AYAGLSNVIGGIYLTGPKYAGGNGNIEWVDGKPFVSIRESLWSADPTEIAARINLLPA
                     DPESPEGYTIVNVHPWSHTMADVKAVVDKLEPHVRVVNPEEFFLQLIFNQDGRFPTTR
                     RSVEANITNPINREAVRGIIVVNGNASSAAPIRSVQEAVGDGPWLEAEPVEEDFRCWQ
                     ASVDTGNVEDGLHALRVRVTTEDGEVYYCSEEIVVRNDSESGTVVGTVMRAGLPVEKA
                     EVELMGRHAVTDSHGSFEFEFVPSGIVE"
BASE COUNT         1325 a         1399 c         1569 g         1424 t
ORIGIN      
        1 gccaggtcgt acttcaaggc atcatgattt cactttgagg ttgtttacct atagaagtac
       61 atcaatcgag tgcgcgaaga gaggaactcc cctcgcgcac tcgattggct tttgccgtgg
      121 attagttatt catgggcatc tatcgctaca gtgtgcggca gatgcgtcaa aaagctcctg
      181 gccctgtcag ttcccgctca ggcccgaagt ctgtgactcc ccatccgagc gagataatct
      241 tgggtatgtt agccagatac atatggctgc ccagcaggac ggaatttggc ccgcagctgt
      301 atactgcaac gggactgcct gtatgcccgg tgtgcaccca acctatttcc gcggatgttc
      361 cgtacgcgct gccactcgta atgccgagac ctccagtctc gtggtcggct acgactacca
      421 caagcgtatc gtctcttccc gaagcgaagt cgacggcgac ctgaacggcg gtttcaaaat
      481 caagcatttc tgtgactact ctgtagccat cattattatg cccgccccca tctatctggt
      541 ctccttccac catgaggaag aacccatttt cattttggga aaggcgcctg attgcctcct
      601 ctgttaggtc ccctagagtg tagttccgcg atgccgcccc agtgagtgaa cccgaattga
      661 gcagagccca tgccctaagg ttgttatctg ctttcaggtc atccacattg tcgaccactg
      721 tgaatccttc gtcctgcagc tcttttagga gattgcgtcc atcgattcgt gctccgttgt
      781 cggttcctgg cacaaaatgt ttgcggcctc cccccattac tacgtcaacc ctgttatcga
      841 agatgcctgt ggatatggca gggtcatcgc ctcgatcgcc tgcatgagac ccgaaactgg
      901 ccggagtggc tccggtcacg ctgtccgttg ttatgattcc ggtggacttg ctgagttctt
      961 tggcaagctc aagaatggtt ttgagaggga cgccgtcagg atcaacgctg atggtcctgt
     1021 tgtttaccca gtaccctgtg gccagttgag tgccggctgc tgcggaatct gttacaagcg
     1081 aaccacgcga atgggtgttt gcaagaccaa caatgggcat ctgttcgctt ggaaggcggt
     1141 agttggggcc tccggcacag atgcgggcca gggttcggtg gctcaggcca gtgccatcgt
     1201 tgatgagaag aatcgcattc ttggctggtt tcactggttc atgcagatac agaaccggaa
     1261 gtctgtcata gaagtcttgg cttccgagat aaacgatttc tcggattggg gcctccaagg
     1321 tgtcagattc ctcatatgca ggggcaatgc tttgaagggc tgttataggt gtcacatctg
     1381 cccgatccag ctcatcggcc cataatggga cgtagtcgat gacgagcaat tgcccaccgc
     1441 ccagatcgag cgccacaacg gatctagtct ctatgtccgc cccggttgaa tctttttcta
     1501 cggtttccca agttcctcgg gtgtacttgt agtctaatat ctggccttcg gctatttcca
     1561 gtgtcgttgt ccatacgttg ttgccaatat gtctcatggc gacgcttgcg gcatcgcgtt
     1621 ggttagctgc ctcgcttcct atgtacacta tgtcaccagt tggagtgcga tccggaacta
     1681 ttacgtaaaa gtccactttg atcggagaag cacctacagc tgctgtgcta gctgttgcca
     1741 gcaacagaca gaggacgatg aagcacaagt gccggttgaa accatgtttt ctgtccacaa
     1801 gaagatcctc cttttattgt tccggctaca cgtatagcct tgttggtatt acaggcgaaa
     1861 gcatgcttta tcgcagtttg gcttgacaga gataaagcaa atgtccgtac atatgccgtt
     1921 atctatttca acgtttcaga gcataatcct tcaaagagtg gacgatcatt ttagtcgaag
     1981 actccctggc gttactgcgg atatcctata cgtgccaggt aactgtggcg ttgatgcgcc
     2041 ttaagtttta aagaggagcg ggaaggagtt cacgtgggcg cgtagaatag gaacaatgaa
     2101 tgtccgtaca ttacaatgcc gccgactgtc cgtgaacagg cggcgaactg gagaaaacgg
     2161 gggcgggatt ggcgacaaat tactccgcca agtgttgtcc cgtaattatg gttgtaaagg
     2221 tttgatgcga tgagcaatgt ccgtacaaac ttttgattgc tgcactaact tgtgcatgtg
     2281 ctactaaaac atgggggtga ctctctgatg ggcgctaaca gcgctaagga tcgccgtttt
     2341 gaccgggtca gacgatggat gttgttggct gtgatcatag tttcactatt gggctgttca
     2401 agccatggat tggctgcaaa gactagactt attatatggt ggccacaggg ctccacagag
     2461 aaggcggtat tgcaagcggc aatagccgac tatgccaagg tcaatccctc tgtagagatc
     2521 gttccgcagt ggcaacccga ctccgatgat acgcagaagc tgaagatagc ctatgtgggc
     2581 gggaacgcgc ctgacatcgt caggattgac cacgtgtggg tccaggctct cggttacaag
     2641 cgtatgttca ccgacctagt tccctatggc gcaaattcag tgaagaacat gtttttcgat
     2701 cccacctggc aagcgaatac atacaaggac aaagtgttcg ccctgccttt cgacgcaaac
     2761 gtgatttgcc tgatgtacaa caatactctt ctcaaatcaa ccggcaccac cgttccaact
     2821 acctacgacg agctgatagc tgtcggaaaa gccaatacca gagatatcaa caatgacggc
     2881 aagatagata aatggggata caccattccg gtaagtgccg gaggcagtgg atggctccag
     2941 ttccagtggc tcacatggct atggaggatg ggcggagagg ttctcaatga agactggact
     3001 cgcgccgttt tcaacagccc tgagggcgtg aagtctctgg aaatgatgat agacgtagtc
     3061 aaaaagcaca aaattgctcc tgatggcgca tattacgaag gcgattttta caatggcaac
     3121 gttgccatga tcgatatggg accgtggtcc atgagcggca gagatcccaa ggtttttggc
     3181 tttgctcctc tgcccgtttt gaatccgcgt gtgggcgagg ctactggact cgggctctat
     3241 tcgcttgcga ttaccagcag ctgcaagaat ccgaaggcgg cgtacgattt tgtcaagttc
     3301 gtgacgacga cgccaaaata ctcggcgatg tggtccaagg gcacatactt gatgccaagc
     3361 ctgaagacgg cttacagcga cccgctatat aggaccggat attggtctgt gttcatgagc
     3421 cagcttccca aggctaagat gcgtccgggt acgcctgcct ggcctgaaat agcgggatat
     3481 atctcggatg cgatacaaag tgttctggcc gaaaaggcca ctgctgccga ggcactaaac
     3541 aaggcggctg agttgagcaa taaggctctg tctaaagtca agtagccttg gctacgtttc
     3601 tgcggggcat ggtggctcat ggaggggcca tcatccccgc aattgttgag aacgccagct
     3661 ttgcagtgaa gatgataggg gagatatatg tgatctgcag ccaagcagcc ccgtttgtaa
     3721 aagcagtttc tgttatcgtt gcgattgcta ttcttctggg ttcagtttca ggcacgtgtt
     3781 ctgcagcgat gcaaatggat ggcgttagcg aagtgagcca tcgttttccg tgtattgccc
     3841 ggcctgaacg tctgtacgtg atttccaagt ctgcgatgac cgatgctgag cttctgatgg
     3901 tctcatcatt gcaaggaata gtggcccaaa ctaggccgga aatatacatt gacagcggat
     3961 cggcttatcg cggttggctg gaattgattc gtgccgaaca tggcatccct atgcagtacc
     4021 atgatgaggc ggaatggttt gtagaccact acaaggacat tttcaaagag aatggttatg
     4081 ttcttgcgga tctcaaatcg gaatgcgtca atgtagccac aaccgtgtcg ggcctcctgg
     4141 gggcaccgat tgtggatcaa tccttgcttc cactcatgtg tgtgcgaggt ttcgagatgg
     4201 cgatcgatgt gcgggacaag gatgagtgct gggcgtttga aaactacaag gataagttga
     4261 atccaaacat tctcttccgg caggaccctc tcaaactaca gcttagagat tacgcaatag
     4321 cctgcaaggg ctttgtgttc tatcaacctg acaatgatga attcgtttca acagtctatg
     4381 actgggctca ggaagatgcc cctgtgttgg gatggggtcc aggatttgag gacgatcacg
     4441 tggctaaagc gtccgagcac aatcacatga ctattcccgc tgattgggcc atgaatctta
     4501 gcgctcttgc cgggatcgga cacgggagcc ttcaacaacg caatcacgtg gccaatatca
     4561 ggttcgagcc agatgttcac tacgtcacat tcgtgatgag cgatggcgac aatgtgcagt
     4621 ggcttttggg cgacatggca gtcaagccgc atcactatgg aagcgctgtg cgggggaaat
     4681 tcccaatggg tttcacgatt tctcctagcc taatcggact ggcccctgcg gcaatgggat
     4741 ggtactatga caccgcaaag ctcgactatt tcgtggcagg cgtgtctggc actggataca
     4801 tatatccttc taagtatcct aggctcgact ccttcgcttt cgaactcgac caataccttg
     4861 ccatgtccga cctttcaatt gtgagcatat tggaccagtg tcctatggga tcgaaggaat
     4921 tccacgatct tgctcaagcc tatgcgggcc tttctaacgt gattgggggc atatacctca
     4981 ccgggccgaa atacgcgggt ggaaacggca acatcgaatg ggttgacggc aagcctttcg
     5041 tttccataag ggaatcctta tggtcggccg atcctacgga gattgcagca aggataaact
     5101 tgctccctgc cgatccagaa agccccgagg gatacactat cgtgaacgtg catccgtgga
     5161 gccatactat ggccgacgtc aaggccgtcg tggacaaatt ggagcctcac gtgcgcgttg
     5221 tgaatcccga ggagtttttc ttgcagctca tattcaacca agacgggagg tttcccacga
     5281 cgcgccgcag cgtagaagct aacatcacca accccataaa tcgagaggct gtcaggggca
     5341 tcatcgttgt gaacgggaat gcttccagtg cggcgccgat aagaagtgtg caggaagcag
     5401 tgggtgatgg tccatggcta gaagctgagc ctgtggagga agatttccgt tgctggcagg
     5461 catctgtaga caccggcaat gtggaagatg ggctgcatgc cctgagagtc agggttacaa
     5521 cagaagacgg cgaggtctat tattgctctg aggagattgt ggtccggaac gacagcgaaa
     5581 gcggtaccgt ggtcgggact gtgatgcgtg cgggcctccc agtcgagaag gctgaagttg
     5641 aattgatggg gcgtcacgca gttaccgatt ctcacggtag ttttgaattt gagttcgtgc
     5701 cgtcaggaat tgtcgaa
//
LOCUS       DAOSAI010000126         5713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8942_length_5713_cov_3.858254, whole genome shotgun sequence.
ACCESSION   DAOSAI010000126 DAOSAI010000000
VERSION     DAOSAI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8942_length_5713_cov_3.858254"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PLM83_05490"
     CDS             <1..832
                     /locus_tag="PLM83_05490"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOK70893.1"
                     /translation="PAARPQRGLGAASIVYEMLAEGGITRFLAFYSGRSSVEIGPIRS
                     VRPYIVDIASGHGTVLAHCGGSQEALAIIAESNYPSVNELVQGSAFYRDNTRRVPHNL
                     YAKAESILDCARRMGYRTDFAESGYVFRKREARGNSNPIEVTIRYSPGYSVQWEYDAK
                     TSLWLRSINKKPHVDANTGQQIAAANVIIEVVPTRVLDDEGRLSMELVGSGEVVALSA
                     GEVMCGRWVRLSGTTDPAVYVAETGTPIELCPGNTWVQIVSEANMVEINSGQGELGGG
                     "
     gene            874..1275
                     /locus_tag="PLM83_05495"
     CDS             874..1275
                     /locus_tag="PLM83_05495"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOK70894.1"
                     /translation="MSADAEEMLVRAAREAREAAYVPYSGFRVGAAVLAESGRIYAGC
                     NVENASYGGTICAERTAFVKAISEGERGFLGVAVCGDTPTPISPCGICRQFMAEFGLD
                     IPIIMAGEGEARSTMTVRELLPAAFTSEDME"
     gene            1276..2160
                     /gene="era"
                     /locus_tag="PLM83_05500"
     CDS             1276..2160
                     /gene="era"
                     /locus_tag="PLM83_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOK70895.1"
                     /translation="MKSGFTAIVGKPNVGKSTLLNRLIGTKVSIVSDKRQTTRNRINA
                     IANLPDAQVIFIDTPGIHKPHHGLGKRMVQAARTAMEEVDLILFVTDATSPARDDDRT
                     VASYIRDLGTPAWLVINKKDLVDEETLAVSERACRALGEFDRVKILSAETGENLEGFI
                     GEIADAMPEGPMYYPPDMVVDRPEEFFIAELIREKLLELTREEVPHSIAVVVEEMIPR
                     EDRDIVDVSATVYVDRESQKGIVIGAGGSVLKEAGARARLEAERLLGSQINLKTWVKV
                     RRGWRDDEAILDRLGYSE"
     gene            2296..3318
                     /gene="queA"
                     /locus_tag="PLM83_05505"
     CDS             2296..3318
                     /gene="queA"
                     /locus_tag="PLM83_05505"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOK70896.1"
                     /translation="MRVEDFDYELPEELIAQTPIERRDASRLMVLRREDNSIEHRSFC
                     DLPEFLHSGDVLVLNDTRVIPARLWGTRSTGGKVELLLLQEIEADVWECLARPGKRAH
                     VGEKLSFGKGRMIGTVLDKTEYGGRVVSFEAIEGVDKVVDEIGEMPVPHYIKTDLKDA
                     ERYQTVYARERGSAAAPTAGLHFTPELLDEIASCGVVIVRVTLHVGLGTFRPVKTEVV
                     EEHVMHSEHYHVSKEAADAINEARASGGRVVAVGTTSVRTLETVARPDGTIAPGEGWT
                     SIFIYPGYTFRAVDAMVTNFHLPKSTLVMMVSAFAGREFILSAYREAVAERYRFFSFG
                     DAMLIL"
     gene            3388..4494
                     /gene="tgt"
                     /locus_tag="PLM83_05510"
     CDS             3388..4494
                     /gene="tgt"
                     /locus_tag="PLM83_05510"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOK70897.1"
                     /translation="MEFTIEKRSEETMARAGVLSTGHGDILTPVFMPVGTQASVKTVS
                     PDELSQIGAGIILSNTYHLYLRPGHELIAEAGGLHSFMSWDGAILTDSGGYQVFSLGP
                     LRTITERGVEFRSHIDGSPHMFTPERVMEIEMALGADIAMCFDECTPYPCDYEYARDS
                     AAMTARWAERCLEAHSREDQALFGIVQGSVYPDLRKRSAADISSLGFPGYGIGGLSVG
                     EPKDVMYAILDETVPCLPEDKPRYLMGVGSPDALLEGVARGVDMFDCVLPTRVARNGT
                     VFTREGRIIVRDAAYAEDFRPLDPGCDCYACRNFSRAYIRHLLKAGEVLGIRLTSIHN
                     LRHLVRMMEEVRSAIIKGTFSEYRKEQWRIWYNN"
     gene            4532..4867
                     /gene="yajC"
                     /locus_tag="PLM83_05515"
     CDS             4532..4867
                     /gene="yajC"
                     /locus_tag="PLM83_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOK70898.1"
                     /translation="MEAQAGQAPSLLGALLPFVVLMLLFYFMIIRPQKKQQQQRDAMI
                     AGLRKGNRITTIGGIYGEIVDIKDDAVLLNIASQGERLIVRVAKWGVQDVLGKDNVAK
                     DKETEEEIS"
     gene            4901..5683
                     /locus_tag="PLM83_05520"
     CDS             4901..5683
                     /locus_tag="PLM83_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70899.1"
                     /translation="MTSKRWTALGALLILLVAIPFSAGASSTATAEEVAREYLMAFQE
                     GDWRRAEELADDAALSWVEYVGSREGKSDGIVSIAAVETANLGDSVRAKVYYTNSDGQ
                     LRVRYLKLRDIAGRFRVVDDKMLGGEWISLSYRKGLFPTAKEIGGVRVRVVGMLEVPP
                     EIKFDLIVENVSGPDNCLIYPSLEAFYVVEADGAIRGKYFAQPPAVVPEKPLPKGVAM
                     RTYAIMPFWSVDPKFSGRTIKSLSWTLFIPFGPIDQYAFDYI"
BASE COUNT         1317 a         1319 c         1775 g         1302 t
ORIGIN      
        1 gccggctgca aggccccagc gtggtcttgg agccgcctcg atagtctatg agatgttggc
       61 agagggaggc attacgcgct tccttgcctt ctactcaggt cgctcatctg tcgagattgg
      121 ccccataaga agcgtccgcc cgtacattgt ggacatcgcg tctggccacg gaacggtatt
      181 ggcccattgt ggaggcagcc aggaagctct tgccattatt gccgagtcga attacccatc
      241 tgtcaacgag cttgttcagg gaagtgcgtt ctaccgcgac aacaccaggc gcgttccaca
      301 taatctgtat gcgaaggccg agtcaattct ggattgtgct cggcgaatgg gttatagaac
      361 ggattttgcg gaatcaggat atgtattccg aaagcgggaa gctcgcggta atagcaatcc
      421 gattgaagtg accataaggt attcgcctgg atactcagtg caatgggaat acgatgcaaa
      481 aacgagttta tggctgcgtt caatcaacaa gaagcctcat gtggatgcca ataccggaca
      541 gcagattgct gcggcaaacg tcatcattga ggtagtgcca actagagtcc tcgacgacga
      601 aggtaggctc tctatggaac ttgttggttc cggagaggtg gtggcgctta gtgcggggga
      661 agtgatgtgc ggccggtggg tacgtctgtc aggcacgact gatcctgcgg tttatgttgc
      721 ggaaacaggg acgccgatag agctgtgccc cgggaatacg tgggtacaga tcgtgtcgga
      781 ggcgaacatg gttgaaatca attctgggca aggtgaactc gggggtggat aaaagtgtca
      841 ggcagtgcca caggcacaga tgaggcgcgc gttctgtcag cagatgctga agaaatgctt
      901 gtccgggccg cacgcgaggc gcgggaagcg gcctatgttc cgtattcggg cttcagagta
      961 ggggccgcag tcctcgctga aagcggacgg atatatgcgg gctgcaatgt ggagaacgct
     1021 tcgtacggcg gaaccatatg tgcagagcgg accgcttttg tgaaggcgat ttctgaaggg
     1081 gagaggggat ttcttggagt tgcggtgtgt ggagacacgc ctacaccgat cagcccgtgc
     1141 gggatctgtc gccagttcat ggcggagttt ggtctcgata ttccaataat catggccgga
     1201 gaaggtgaag cgcgttcaac tatgaccgtg cgtgagctgc ttccagcggc cttcacatcg
     1261 gaggatatgg agtagttgaa gtcgggattt acagcaatag taggcaaacc taatgtaggc
     1321 aagtcgactc ttctgaatcg tctgatcggg accaaagtct cgatagtttc tgataagcgt
     1381 caaacaactc gaaaccggat aaatgctata gccaatctcc ctgacgcgca ggtaatcttc
     1441 attgacacgc ctggaataca caaaccccat cacggccttg gaaaaaggat ggtccaagcc
     1501 gcaagaacag ctatggagga ggtagatctc atactctttg tgactgatgc cacgtctcct
     1561 gcccgagatg acgacaggac tgttgcgtcg tacataaggg atcttgggac ccctgcatgg
     1621 ctcgtcataa acaagaaaga ccttgtagat gaggagacgt tggcggtttc agagcgcgcc
     1681 tgccgtgcgc ttggcgagtt tgaccgcgtc aagatcctct ctgcggaaac aggagagaat
     1741 cttgagggct tcattggtga gatcgccgat gcaatgcctg aagggcccat gtactatcct
     1801 cccgatatgg ttgtagaccg tccggaggag tttttcatcg ctgagctcat tcgcgagaag
     1861 cttctcgagt tgacccgcga ggaggtgcct cattccatag cggtggtagt tgaggagatg
     1921 atccccaggg aagatcggga tatcgttgat gtaagtgcga ccgtatacgt tgaccgtgaa
     1981 tctcagaaag gcattgtgat tggggccggc ggtagcgtgc tgaaggaggc gggcgcacgt
     2041 gccaggcttg aggctgagcg cctgcttggt tcgcagatca acttgaagac atgggtcaaa
     2101 gtgagacgtg gatggcgaga tgacgaggct atccttgaca ggctggggta ttcggagtaa
     2161 ggttgctaac ggcgtttcaa gctcgggaaa tgccgagtgt tgcggctgta gacagcttgc
     2221 agctgcggtc ggatcggcct gttcaaatgt cctgatttga ggctgccaca gatgggccgg
     2281 agaagggata tacatatgag ggtagaggat ttcgactatg agcttcctga agaactcatt
     2341 gcgcagacac cgatagagcg gagggatgcg tcccggctca tggtgttgag aagagaggac
     2401 aactcaattg agcacaggag cttctgcgac cttccagaat tcctgcattc aggcgatgtc
     2461 ttggtcctga acgatacaag ggtaatcccg gccaggctct ggggcacgag aagcacaggc
     2521 ggcaaagtag aactcttgct gctccaggag atagaggcag atgtgtggga atgcctggcg
     2581 agaccgggta agcgtgctca tgtcggcgaa aaactgtcat ttggcaaggg ccgcatgatc
     2641 ggcactgttc tcgataagac ggaatatggg ggacgcgtgg tgtcatttga ggcgatcgag
     2701 ggcgttgaca aggtggttga tgagataggg gagatgcccg tgccccatta cataaagact
     2761 gacctgaagg acgctgagag gtatcagact gtgtacgccc gcgagagggg ttctgctgcg
     2821 gcgccaacag cagggcttca ttttacgccg gagcttttgg acgagatcgc aagctgcgga
     2881 gtcgtaatcg tccgtgtcac tctccatgtg gggcttggta ctttccgtcc tgtaaagaca
     2941 gaggttgtcg aggagcacgt aatgcactca gagcactatc atgtatcgaa ggaagctgca
     3001 gatgccatta atgaggcaag agcttcggga ggccgagtag tggcggttgg aacaacgagc
     3061 gttagaacgt tggagactgt tgcccggcct gacgggacca tcgccccggg cgaggggtgg
     3121 acaagcatat tcatatatcc cggatacacc tttcgcgcag tcgacgccat ggttacgaac
     3181 tttcatcttc ctaagtcgac tctggtgatg atggtatcag cctttgccgg cagggagttc
     3241 atactcagcg cgtatcgcga ggccgtcgct gaacgctatc gctttttttc atttggcgat
     3301 gctatgctca tcttgtgatg cctgtcttgc atcggttagc gggcacaaat gcagtctagt
     3361 gtttatggag tgatgcttat tacggctatg gaatttacta ttgagaagcg atctgaggag
     3421 actatggcga gagcgggagt gctctccact gggcatgggg atatactcac gcctgtgttc
     3481 atgccagtgg gcacgcaggc ctcagtgaag acggtttctc ctgacgagct ttcccagata
     3541 ggcgccggga tcatcttgtc caacacgtat catctgtact tgcgccctgg tcacgagttg
     3601 atagcggagg cagggggact acattcgttc atgtcgtggg atggagccat cctcacggat
     3661 agcggaggat accaggtatt ctcgctcggg ccccttcgaa ccattactga gcgcggcgtg
     3721 gagttccgtt cccacattga tggctctccc cacatgttta ctccggaacg ggtcatggag
     3781 atcgagatgg ctttgggcgc cgatatcgct atgtgtttcg acgaatgcac gccatacccg
     3841 tgtgattacg agtacgccag ggattcagcg gcgatgactg ctcgctgggc cgaaagatgc
     3901 cttgaggctc attccagaga ggaccaggcc ctcttcggga tagttcaggg atctgtctat
     3961 ccggacctga gaaagcgctc tgccgctgac atatcgtctt tgggatttcc cggttacggg
     4021 atcggtgggc tctctgtagg ggagccgaaa gatgtgatgt acgccatttt ggatgagacg
     4081 gtgccctgcc tccctgagga caaacccagg tatcttatgg gagtgggatc gcccgacgca
     4141 ttgctcgagg gggttgcccg cggagtggac atgttcgact gcgtcttgcc gaccagggtt
     4201 gcgagaaatg gcacggtttt cactcgtgaa ggccggataa tagttagaga tgcggcctat
     4261 gctgaggatt ttcggccact cgatccaggc tgcgattgct atgcgtgcag gaatttttcc
     4321 agagcctaca tacgccacct tctaaaagcc ggcgaggtgc tgggtatcag actcacatcc
     4381 atacacaacc tcaggcacct tgtgaggatg atggaagaag tgcgttcagc gataattaag
     4441 ggcacttttt cggaatacag gaaggaacaa tggcggatct ggtataataa ctaggacaca
     4501 acctgaatgg gaaaatggag gtaagcagcg tatggaagca caagcagggc aggccccaag
     4561 cctgttagga gcattactcc cgttcgtcgt actgatgttg ctcttctact tcatgataat
     4621 ccggccacag aagaagcagc aacagcagcg tgatgcgatg attgcgggat tgcgcaaggg
     4681 aaataggata acaacaattg gcggaatata cggagagata gtcgacatca aggacgatgc
     4741 agtgctgctc aatatcgcca gccagggcga acggttgatt gtgcgagtgg ccaaatgggg
     4801 agtccaagat gtgctgggca aggataacgt ggccaaggat aaggaaacgg aagaggagat
     4861 ttcataggac gaagggcgat tttgaggagg cctctacata atgactagca agagatggac
     4921 ggcgttgggc gctttgctca tcttgcttgt ggcgattccc ttctcggctg gagcttccag
     4981 cactgccacg gctgaagagg tggcacgtga gtatctcatg gctttccagg aaggggactg
     5041 gcggcgtgct gaggaacttg cggacgacgc agctctctcg tgggtggaat atgttgggtc
     5101 tcgcgagggc aagagtgacg gcatagtctc tattgcggcc gtggaaaccg caaacctagg
     5161 tgatagtgtc cgtgccaagg tctactacac gaattctgac ggtcagttga gagtcagata
     5221 cctcaagctg cgcgacattg ccggacgctt cagggtcgtg gacgataaga tgcttggcgg
     5281 agagtggata tcgctttcct atcgaaaagg gctgtttccc acggccaagg agataggggg
     5341 agtgcgagtt cgggttgttg gaatgctgga ggtaccgcct gagatcaagt tcgatctcat
     5401 agtggagaat gtgtccggcc ccgacaactg cttgatctac ccttcattgg aggcattcta
     5461 tgtagtggaa gccgatggcg caatccgggg aaaatacttt gcgcagcccc cggctgttgt
     5521 acctgagaag ccacttccga aaggtgtggc gatgcgcacc tacgcgataa tgccgttctg
     5581 gagtgtagat ccgaagtttt cgggcagaac cataaagagc ctttcatgga ctctctttat
     5641 ccccttcgga cccatagacc agtacgcatt cgattacatc tagttttaag cgaatgcata
     5701 tccgggccgc gtc
//
LOCUS       DAOSAI010000127         5680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_8991_length_5680_cov_3.573867, whole genome shotgun sequence.
ACCESSION   DAOSAI010000127 DAOSAI010000000
VERSION     DAOSAI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8991_length_5680_cov_3.573867"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1155)
                     /locus_tag="PLM83_05525"
     CDS             complement(112..1155)
                     /locus_tag="PLM83_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70900.1"
                     /translation="MIIRSAEPRDDAVLLGIESVAGQGDQIRLIGERDSFFSRARQFE
                     NTLLFVGENETTGKLHGVVAASVLPVRVEGRERIGAYMFDLRSNPETNKGLSRAMYLI
                     WKGLEDRLLQCGTEFIYGLIKEDNSRSYSIATRMGATPRGEKRFLTFPVFRRIRVSRE
                     VQTISHVDAMEEHLAAAKHYDGPYQLWPIMDDFSYMQGLLDKYLVAKMECGSSSLKIW
                     DSSLNCAYRVSAMPRPFEILGTVLRAASHLVPLPKIPMPSQKLRIWNVYDIITRPGQR
                     SEAQALIAKANNLALEHGVDFLIITVDAEEDELRWLRRRCLMPLDYKLMVKEYNPVPE
                     FFGKTYFDPRYLG"
     gene            complement(1264..2469)
                     /locus_tag="PLM83_05530"
     CDS             complement(1264..2469)
                     /locus_tag="PLM83_05530"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HOK70901.1"
                     /translation="MAIGFEQGALHIKRLSLVEAVMMLVGGNVGAAILSLPYAARASG
                     YLGAVTVAVITTVFSAISHLYIVEIMLRTKSPGQLVGLVRTYMFDSRHGKLYLLLVSS
                     LTIGLVIPSLTAYVIGGGAIISSLLALPAWAGHLLFLLPGAAVVWLGLEAAGLAQGVS
                     SIAMMSVLLLFAGISMRHPSFDPARLARFSAKPLAAALPVGIFTSMSQSLVPEIVRGL
                     AHDPRSAPTAIKWGLGINLLFLLIFPMAIFGLQDPGEISEVVTISWGQALGPAIYFAV
                     NLFALLALLTSFWGGAGAALGNVVEIFRFKSEWDIGARLAAFAITITPSVVLASVGNF
                     DFVDMIQTAGSVGGVILAVLPIFILAKARKRSEREPEYQVGIAFSPIVRVALFVFYVG
                     VLIYAAFAR"
     gene            2650..3645
                     /locus_tag="PLM83_05535"
     CDS             2650..3645
                     /locus_tag="PLM83_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70902.1"
                     /translation="MVSLPFTIGERFGLLAALIVAAVAVVVSPWACGAQQLACIHVAE
                     ACLTAIPSFPGICHASVLLPETVRKEFMFVQGGKVYCVELGGLEEPGDAGTESVSIIR
                     FGLGAEPENIGKIEGNAMSVWLLGDINKDGCMEMLVRRSEGHFAYKTSIVYIDQKDRM
                     RSFDISGYTDSQGSTDLFLTPESFADLDGDRIPEIVATVSPKGFPSLLGAKPPQISLV
                     FKLNGTTYSPTGQRFAEIYRFLAAQTRCELASYWDFEVVEYPQSYVRSCCELLLYHAL
                     AGDADVGAEEFDKAIRILQDAGVEITEDDNRALKEFGREREAIVHEIMRGLLTGP"
     regulatory      3868..4000
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      4001..4069
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4166..4600
                     /locus_tag="PLM83_05540"
     CDS             4166..4600
                     /locus_tag="PLM83_05540"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HOK70903.1"
                     /translation="MDSRTYRVITNNPLVAATWPGSVEWVSGSVKDVFRCVRDYVHAG
                     HVLLTHPLSGSVKPNESPYKSVIITDYPASAMVDLDSLRIIEGAMVVLHNLGAPRFTH
                     IPPRVDDDYQLIDCDLMASAIESLHDAAWNGPVEGCKTEAFQ"
     gene            4634..>5680
                     /gene="trxB"
                     /locus_tag="PLM83_05545"
     CDS             4634..>5680
                     /gene="trxB"
                     /locus_tag="PLM83_05545"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOK70904.1"
                     /translation="MAAEDMKTYDVIIVGGGPAGLSAALYASRSKLSTLVLEKAKPGG
                     QAGTTEWLENYPGFPGGVKGPELMEHFAAQAREFGAEIIKDDVASVDFSGEKKLVCTA
                     GGGQYSASAVILCPGAEPRTLGLPGERELRGKGVSYCATCDGDFFTDLDIIVIGNGDA
                     AVEEAMYLTRFAESVTMIVVHDEGVLDATAVIRDRAFKNPKMKWVWNSVVDEIRGDGI
                     VESVIIRNLKTNERTELETNGVFVFVGTVPNTQFLSGAVEMDKRGYIIANEQMETSVD
                     GVYCAGDARVKYLRQVITAAADGAIAAVAAEKYIHEEEGFREMVLAPASPVMVCFWSP
                     TSQESLDFMPAVERI"
BASE COUNT         1323 a         1435 c         1635 g         1287 t
ORIGIN      
        1 attgcccgct cttcgctggg cgaatcccgc gttgcggccc gggaacccca ggctcagtct
       61 ttaaaatgcg tctgtaaggc ggggaggcgc catgtggtgc ggggacgatg gttacccgag
      121 atatcttgga tcgaaatatg tcttgccgaa aaactccgga acagggttat actccttaac
      181 catcaatttg taatcaagcg gcatcaggca gcggcgcctg agccaccgga gctcgtcctc
      241 ctcggcatcc acggtgatga ttagaaagtc taccccgtgt tccaaagcta agttgtttgc
      301 ctttgcgatc agcgcctgcg cctcgctgcg ctggcccggg cgtgtgatga tatcgtacac
      361 gttccagata cgcagctttt ggctgggcat ggggattttg ggcagaggga ccaaatggct
      421 ggctgccctg agaaccgtgc cgagaatctc gaagggtcta ggcatggcag agacgcggta
      481 ggcgcagttg agtgaagaat cccatatctt cagagaagaa gatccacatt ccatcttggc
      541 cactaggtat ttgtccagca gaccttgcat gtagctgaaa tcatccatga tgggccagag
      601 ctggtatgga ccgtcataat gcttcgctgc agcgagatgc tcttccattg catccacatg
      661 tgaaatcgtc tggacctcgc gggagactct aattcggcgg aagaccggaa atgtgaggaa
      721 acgtttctca ccacggggcg ttgctcccat gcgtgtagca atgctgtacg agcgggagtt
      781 gtcctccttt atcagcccgt atatgaactc cgttccacac tgcagaaggc ggtcctcaag
      841 gcccttccat atcaagtaca tcgccctgga cagtccctta ttcgtctccg ggttggagcg
      901 gaggtcgaac atgtatgcgc ctattcgctc tcgtccctct acgcgaacgg gcaggaccga
      961 ggcggccaca acgccgtgta gcttgccggt ggtctcgttt tcgccgacaa aaagcagcgt
     1021 gttttcaaac tgtctggccc tactgaaaaa actgtcccgc tctccgataa ggcggatctg
     1081 atctccctgc cctgccactg attcaatgcc gaggagaaca gcatcgtccc tgggttcagc
     1141 agacctgatg atcatcgtga cccctcctga gatcgcagac ttcggtaatt tcaggttcgg
     1201 aacgagttgc gccatggcca ccaggccaaa tcaaagattc caccttacct caaagtcgtc
     1261 ggactatctg gcgaatgccg cgtagatcaa tacgcctaca tagaagacga agagcgctac
     1321 tctcacgatc ggggaaaagg cgatgcccac ctggtactct ggttcacgtt ctgagcgttt
     1381 ccgcgctttt gcaagaatga agatgggcag cactgccagg ataacgcctc ccactgaacc
     1441 tgcagtctga atcatatcaa cgaaatcgaa gttgcctaca gaggctagga cgaccgatgg
     1501 cgtgatagtg attgcaaaag cagccaaacg agccccgata tcccactcgg atttgaaccg
     1561 gaagatctct acgacatttc ccagcgcagc gccggcgcct ccccagaagg atgtgaggag
     1621 tgccaacagg gcgaagaggt tcactgcgaa gtatatggcg ggaccgagcg cctgtcccca
     1681 cgagatcgtt acgacctcgg aaatctcgcc ggggtcttga agcccgaaga ttgccatggg
     1741 gaagatgagc agaaacagga ggttgatccc gagtccccac ttgatcgcgg tcggcgccga
     1801 tctgggatca tgggctaacc cgcggacgat ttcggggacc aaagactgtg acatggatgt
     1861 gaatataccc acagggagtg ccgccgccaa cggcttggcg gagaaccgtg ccaggcgcgc
     1921 aggatcgaag cttggatgcc tcattgagat gcctgcgaat aggagcagaa ctgacatcat
     1981 ggcgatgctg gacactcctt gggccaagcc tgcagcctcg aggccaagcc ataccacggc
     2041 agcgcctggg aggagaaaca ggagatggcc tgcccacgct gggagggcaa gaagggacga
     2101 gatgattgcg ccgccgccga tcacataggc tgtgagggat ggtatgacca atccaattgt
     2161 cagagatgac accaaaagca gatagagttt gccatgtcgg ctgtcgaaca tgtaggttcg
     2221 gaccagacca acaagttggc ctgggctctt cgtgcggagc atgatctcta caatgtacaa
     2281 gtgagagatt gcagagaaga cagttgttat gaccgccaca gtcacagccc caagatagcc
     2341 agatgctctt gctgcatacg gcaaggacag aatcgctgcc cccacatttc cgcccacaag
     2401 catcatcact gcttcgacga ggctgagtct ttttatgtga agggcgccct gctcgaagcc
     2461 tatggccatg ctcatcacct acgtttttca gttagccttc taaattccat atattttctc
     2521 atattcctgc tgtcaaatcg gaggattctt ctttaatatg tgcaacttgc aacgccgctg
     2581 cggtttctat actatgagcg gcgatcgatg aatactctct tatgaacaat tcaacgattg
     2641 gaggggtgta tggtgtcgct cccatttaca attggtgagc ggttcggact attagctgcg
     2701 ctcattgtgg cggcagtggc agttgttgtg agtccgtggg cctgcggagc ccagcagctt
     2761 gcttgcatac atgtcgcaga ggcctgtctg actgccattc cttctttccc gggcatctgt
     2821 catgcgtcag tgcttctgcc tgagactgtg cgcaaggagt tcatgttcgt tcagggagga
     2881 aaggtctact gtgttgagct cggaggtttg gaggagcctg gcgatgccgg cacggaatct
     2941 gtgtcgataa tccgttttgg actgggggct gagcccgaga acataggcaa gatagaagga
     3001 aacgccatgt ctgtgtggct cctcggagac atcaacaaag acggttgcat ggaaatgctg
     3061 gtgcgcaggt ctgaagggca tttcgcgtac aagacttcca tcgtctacat agatcagaag
     3121 gatcgcatga gatcatttga catatcagga tacacagaca gccaaggctc aactgatctc
     3181 tttctgaccc ctgaatcctt tgctgatctc gatggcgaca gaatcccgga aatcgtagcc
     3241 actgtgagcc ccaaggggtt cccatctctc ctgggagcta agcctcctca gatcagcctt
     3301 gtattcaaat tgaacgggac gacctattcc ccaaccggcc agagattcgc ggagatatat
     3361 cgcttcctcg cagctcaaac ccgttgcgag cttgcatctt attgggattt cgaggtcgtg
     3421 gagtatccac agagctacgt taggtcctgt tgcgagttgc tgctttatca tgctttggct
     3481 ggcgacgcag atgttggggc ggaagagttt gacaaggcaa ttcgcatcct gcaggatgcg
     3541 ggggtagaga ttactgaaga cgacaacaga gcactgaaag agtttgggcg cgagagggaa
     3601 gccattgtac acgaaatcat gagggggttg ttaaccggtc catgacatat cggcgctgtc
     3661 tcagtctgcg gaccctgtct ttgctgcagg gaccgatctg gcgacctcaa ttcgatgtcg
     3721 gacaggcgtg ggttcaaaag gtttgacaac cgcaagttgg accaggacat atagaagaag
     3781 acaatgatat tcgatatctt atgcaaaaac ttgttgacaa agcgattgtg aagcattaca
     3841 ctaactgtga tggaatagcg tcgccgaagg aacgctgcgg gagagcctca cagcaagcat
     3901 gagatgatcg gcttgcaggt gagcgccgaa ggagcaaagg cactgcacat tgccgtggcg
     3961 gttgcctaaa actttcaggc aaaaggaccg tagctggacg gcatctctgg agaggcgcat
     4021 atcgcaccga aggagtaaat ctctcaggtt ttatgacaga gacgacggca ggattgtttt
     4081 gccatgtcgg ttctctgtct tttctttttg ctgcgtttgc taagactatc gaatatggca
     4141 ttggattttc gggaggacgg cgacgatgga ttctcgtaca taccgcgtga tcacgaataa
     4201 cccgcttgtt gccgctacat ggcctggctc agtcgaatgg gtgtcgggaa gcgtcaagga
     4261 cgttttccgc tgcgtgcgcg actatgtcca tgctggccat gttcttctca cacatcctct
     4321 gtctggaagc gtgaagccga acgagagccc atacaaatcc gtcatcatca ctgattatcc
     4381 tgcttcagca atggtggatt tggattcgct gcggataatt gagggcgcca tggttgttct
     4441 gcataacctt ggggcaccca gattcacaca tattccgccg cgcgtagacg atgattacca
     4501 gttgatcgac tgcgatctca tggcgagcgc gatagagagc ctgcatgacg cggcgtggaa
     4561 tggcccagtt gaaggttgca agaccgaggc ttttcagtag agctagttcg aggcaaggaa
     4621 ggagcgtcta accatggcgg ccgaagatat gaagacatat gacgtgatta tcgtaggcgg
     4681 gggccctgca ggcttgtccg ctgccctgta tgcatcgcga tctaagcttt ccacactggt
     4741 gttggagaag gcgaagcccg gcgggcaagc cggtaccaca gaatggctgg agaactatcc
     4801 agggtttccg ggaggcgtta aaggtcctga gctgatggag cattttgcag ctcaggccag
     4861 ggaattcggc gcagagatca taaaggacga cgtcgcatca gtcgactttt cgggagaaaa
     4921 gaagctagtc tgcactgccg ggggaggtca gtactcggcc agtgctgtaa tactttgccc
     4981 tggtgctgag ccccgcaccc ttgggcttcc tggcgaacgg gagcttcgag gcaagggcgt
     5041 gtcttattgc gcaacatgcg atggagactt cttcaccgat cttgacatca tagtgatcgg
     5101 gaatggagat gctgctgttg aagaggcgat gtaccttaca aggttcgccg agtccgttac
     5161 tatgatcgtt gttcacgacg agggcgtcct cgatgccact gcggtcatcc gtgatcgcgc
     5221 tttcaagaat cccaagatga aatgggtatg gaactctgtc gttgacgaaa tacgtggcga
     5281 cgggatagtg gagtcggtca tcataaggaa cctaaagacc aatgaacgga ctgagcttga
     5341 aaccaacggc gtcttcgtct ttgtgggaac agttccaaac acccagtttt tgtctggagc
     5401 ggtggagatg gataagcggg gctacataat cgccaacgaa cagatggaga cttcggtaga
     5461 cggggtttac tgcgccgggg atgccagggt caagtatctc aggcaggtga ttacggcggc
     5521 tgctgacggc gcaattgccg cggtggctgc tgaaaaatac atccatgagg aagagggctt
     5581 ccgtgagatg gtgctggcgc cagcgtcccc agtcatggtc tgcttctgga gccccacatc
     5641 ccaggaatca ctcgatttta tgcccgctgt ggagagaatc
//
LOCUS       DAOSAI010000128         5647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9046_length_5647_cov_3.363555, whole genome shotgun sequence.
ACCESSION   DAOSAI010000128 DAOSAI010000000
VERSION     DAOSAI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9046_length_5647_cov_3.363555"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /gene="rph"
                     /locus_tag="PLM83_05550"
     CDS             <1..514
                     /gene="rph"
                     /locus_tag="PLM83_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOK70905.1"
                     /translation="GRIAGRTQEIQRLIGRSLRSVVDMASLGECTIWIDCDVIQADGG
                     TRTASITGAYVALVDLLDSMDQWKGKPLPVSDFLSAISVGIVDGQLMLDLCYVEDSHA
                     SVDMNVVMTEGGKFVEVQGTAEGEPFARESLDSMLELAESGIHELCAHQRRALGELAE
                     RVGINGENNR"
     gene            511..1110
                     /locus_tag="PLM83_05555"
     CDS             511..1110
                     /locus_tag="PLM83_05555"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOK70906.1"
                     /translation="MKRLVVATSNRGKFEEIVHILSGLDISVESLSDYPGMVQAEETG
                     ETFRENALEKAIAAARHTHQVALADDSGLVVDALGGAPGVMSARYAGAGASDSDKYRK
                     LLLAMEGVPEGERTARFVCVVAIAAPDGRAWTAEGIVEGIITRVPRGNGGFGYDPVFL
                     VPELGLTFAEIDPETKNRISHRARALARSIPMVQEALGI"
     gene            1145..1221
                     /locus_tag="PLM83_05560"
     tRNA            1145..1221
                     /locus_tag="PLM83_05560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1179..1181,aa:Pro,seq:tgg)
     gene            1468..2457
                     /locus_tag="PLM83_05565"
     CDS             1468..2457
                     /locus_tag="PLM83_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOK70907.1"
                     /translation="MKRPNGEDGWYHVVHDLRPMRTVVGFFLYAELFGGGLRVTHKRA
                     KKVRLDALLVERGLVEDLKSARAWIMSGDVIVDDIREDKPGTLIRPEATIRMRGRNIP
                     FASRGGLKLSAALDAFRVDVSGRIILDAGASTGGFTDCLLSRGAARVYAVDVGYGQLT
                     GKLRQDPRVVNMERTNIGDIVLGQLDPVPDMGVIDLSYLSLSVAIPTVARLLAPSVPG
                     AEPEIIALIKPLFEVAASGPDLTRDQYRAAVERGLRAGEGIGWFARGVMASPVLGSGG
                     TLEFLAHYSLGKVHGDLYEKIDEAVCSGMQLVENAGSPHSRAGGMEESDEGNE"
     gene            2450..3469
                     /gene="pheS"
                     /locus_tag="PLM83_05570"
     CDS             2450..3469
                     /gene="pheS"
                     /locus_tag="PLM83_05570"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOK70908.1"
                     /translation="MNDKVEAVVERALSAIENAHTTAALEEARVRLLGRKGELTTLLR
                     QMKSVDPEDRPKFGALVNSARETIEKELDERARVLVAAEKAARLELERIDVTMPGRAP
                     RIGKIHPLTRGIMDITEIFVEMGFAVVEGPEVETDYYNFEALNTPKYHPARDVQDTFY
                     VNDTVLLRTQTSPMQVREMEKREPPVKVVVPGRVYRRDQVDATHSPVFHQIEGLLVDH
                     DITFTDLKGTLAMFARKFYGEERKVRFRPHYFPFTEPSAEMDISCAICGGTGCRVCKQ
                     EGWIEVLGCGLVHPNVLRAVGYDPDELTGYAFGMGVERLVMLKHGIDDLRLLYENDLR
                     FLSQF"
     gene            3495..>5647
                     /gene="pheT"
                     /locus_tag="PLM83_05575"
     CDS             3495..>5647
                     /gene="pheT"
                     /locus_tag="PLM83_05575"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOK70909.1"
                     /translation="MLVPLKWLSDYVDIDLCIDELADRLTMSGTKVETVTDIGDRLNR
                     IVAGKIVSVSPHESVGRLRVATVDLGPRQARRTLVTAAANIGRGDIVPVVLAGGTIAD
                     GTEIGEVDFSGVRSEGMLCSAKELGISSDGSGILRLPDDIKPGDDVVSALGLADTVIE
                     LEITPNRPDCLCMIGIAREVAAITGATLKMPNTALKEGSERAEELISVKIDAHDLCSR
                     YGARVIQKVSIGQSPIWMQVRLSAAGVRPINNVVDVTNYVMLEVNQPLHAFDYDRMSE
                     HAVIVRRARSGEVIVTLDGEERRLDPEMLVIADPGSALAVAGVMGGLSTEITEDTCTV
                     LLESATFARQSVWKTSRSLKLRTEASSRYEKGLDPETVPLALDRAGHLLETIGAGVAA
                     SGIVDAYPSAAAVRVISTDAARISRRLGVDVSSDDVADSLGRLGFSVRVESVANKAKS
                     EGGSLVVEVPTFRGDVQEEIDLVEEVARMWGYDRIPTTFPAGAHVGGYTYEQKKAMQA
                     KTILTGAGASEALTIPFMSVADLDMLRVGQGDPLRDAIRIDNPMVEEQSYMRTTMLPS
                     MLVAIRTNINRGNRGVCLFELGKTYFPSQSLKRGEELRRGENPAVERQVLVCGMAGAY
                     TPRIWHTRERDYDFYDAKGMLEALLEGMGVDGATYVQTVRPSFHPGRTASIVWEDREL
                     GVIGEVHPDVSEAYGVAGRVYMFELDFARMLEAVD"
BASE COUNT         1288 a         1262 c         1809 g         1288 t
ORIGIN      
        1 cggaagaatt gctgggcgta cgcaggagat acagaggttg atagggagat ccctgagatc
       61 cgtggttgat atggcgtctt tgggcgaatg caccatttgg attgactgcg atgtgattca
      121 ggcagatggc ggcacgagga cggcttctat cactggagcc tatgttgcct tggtggacct
      181 cctggactcg atggatcagt ggaaagggaa gccgctccca gtatccgatt ttctgtcagc
      241 cataagtgtc ggcattgtgg atggccagtt gatgctcgac ctgtgctatg ttgaggactc
      301 gcatgcttcg gtcgacatga acgtagtaat gaccgaggga ggaaagtttg tagaagttca
      361 ggggacagcc gaaggggagc ccttcgcacg cgagtcgctc gactcaatgc tggaattggc
      421 tgagagtgga atccacgaac tttgtgcgca tcaacgccgt gcactgggcg agctagccga
      481 gagagtcggc atcaatggtg agaacaacag atgaaacggc ttgtggtggc cacgtcaaat
      541 cgtggaaagt ttgaggagat tgtacacata ctatctggcc ttgacatctc tgtcgagagc
      601 ctgtctgatt atccaggtat ggtacaagcg gaggaaacag gggagacgtt ccgtgagaat
      661 gcgctggaga aggctattgc tgcagcgagg catactcatc aggtagcttt ggctgacgat
      721 tctgggcttg ttgtggatgc tttggggggt gcccccggag tgatgtcggc gagatatgca
      781 ggcgcaggtg cttccgatag tgacaaatac aggaagctgc ttttggcaat ggaaggcgtg
      841 cctgagggtg agcgtacggc gagattcgta tgcgttgtgg cgattgcagc tccagatggc
      901 cgagcctgga cagcagaagg tattgtggaa ggcatcataa ccagagttcc ccgggggaat
      961 ggcggattcg gttatgatcc tgtgtttctc gtcccggaac tggggctgac cttcgctgaa
     1021 atcgacccgg aaactaagaa tcgaatatcc catcgtgcac gggcccttgc caggtcaatc
     1081 cctatggtac aagaggcatt gggtatttga cgcgtgcccg ggcgtatggt agactcgtat
     1141 tgttcggggc gtagctcagt ttggtagagc gcatggtttg ggaccatgat gtcgcaggtt
     1201 caaatcctgt cgccccgacc attcgttgaa aagtgataat gggtccataa aggtgatgac
     1261 ggagagtagt aggttgggag ttgcccagac agggagggtg tgccgtgact gcaagcgccc
     1321 cgggtaaagc cccatcgcga attcgctccc gaaccgcacg ctgaaagacg ccaaccatga
     1381 gaaggttcat gttcttgtcc ccagtggcgt tctggtaggt gtagacgggg gcctcccgtt
     1441 acaggggcag ggcatgaagt gtgcctgatg aagaggccta acggcgaaga tgggtggtac
     1501 cacgtagtcc acgacttgcg tcccatgcgc acagtcgtgg gcttttttct atatgcagaa
     1561 cttttcggag gcgggctgag ggtgacacat aagcgggcga agaaagtgag gctcgatgct
     1621 ttgctggtcg agcggggcct cgtagaggat ttgaagagcg caagggcatg gataatgtcg
     1681 ggagacgtta tcgttgatga catccgggaa gacaagcctg gaacgttgat aaggcccgag
     1741 gctaccataa ggatgagggg caggaacata cctttcgcaa gcaggggcgg gctcaagctg
     1801 tcagccgctc tcgacgcctt tagagttgat gtcagcggaa ggataatctt ggacgcaggc
     1861 gcttccactg gcggttttac agattgcctt ctttctaggg gcgctgcgcg cgtttacgct
     1921 gtcgatgtag gttacggtca gctcacggga aagcttcgcc aggatccgcg cgtcgtcaat
     1981 atggagagga ccaatatagg ggatattgtg cttgggcagc tcgacccggt accagacatg
     2041 ggggttatcg atctttcata tctatcgctg tcggttgcaa ttccgactgt ggccagacta
     2101 cttgcgccct cggtgcctgg ggccgaaccg gagatcattg ctctaataaa gcctctgttc
     2161 gaggtggctg cttcaggacc tgatctcaca cgggatcaat acagagctgc tgtagagagg
     2221 ggattgcgtg ctggagaggg gatcggctgg tttgcccgtg gggtcatggc gtcgcctgta
     2281 ctgggatcag gaggaacact ggagttcctc gcacattaca gccttggcaa agtccacggc
     2341 gacttgtacg agaagataga tgaagctgtg tgttcaggca tgcagcttgt ggaaaatgcc
     2401 ggtagcccgc atagccgggc gggagggatg gaggagagtg atgaaggaaa tgaatgacaa
     2461 agtggaagca gtggttgaaa gagcgctgtc tgcgatagaa aatgcgcaca cgactgctgc
     2521 cttagaggag gcgcgggtca ggctcctcgg gcgtaagggc gaactgacta ctctcttgcg
     2581 ccagatgaaa tctgttgatc ctgaagacag gccgaagttc ggagctttgg tcaattctgc
     2641 gcgcgaaaca atagagaagg aactggatga gagggcccgc gtgcttgtgg ctgcggaaaa
     2701 ggcggcaaga cttgagctgg agagaataga tgtgacgatg ccgggcaggg cgccccgcat
     2761 cgggaagatt catcctctta cccgaggaat aatggatatt acagagatct tcgtcgaaat
     2821 ggggtttgcg gttgttgaag ggcctgaggt tgagactgac tactacaact ttgaggcatt
     2881 gaacaccccc aaatatcatc cagcccgcga tgtacaggac acgttctacg tcaatgatac
     2941 agtattgctt agaactcaaa cctcgcctat gcaggttagg gagatggaaa aacgcgagcc
     3001 tccggtgaaa gtggttgttc ccgggagagt atacaggcgg gatcaggtgg atgcaacaca
     3061 ctctcctgtt tttcaccaga tagagggcct tctagtcgat catgacataa catttacgga
     3121 tctcaaaggc acgcttgcca tgtttgcacg caagttttac ggtgaagaaa ggaaggtcag
     3181 gttcaggccg cattatttcc catttacaga gccttcagct gaaatggata tctcatgcgc
     3241 tatttgcggg ggcaccggct gcagggtatg caaacaggaa ggctggattg aagtgctcgg
     3301 ctgcggtctg gtccacccta acgtgcttcg cgcggtaggg tatgatccgg atgagcttac
     3361 cggatatgcc ttcggcatgg gagtggagag actcgtgatg ctaaaacatg gcatagacga
     3421 tctgaggctg ttgtacgaga atgacctgag gttcttgtcg cagttttgaa ctggttgaag
     3481 aggggtgaga ataagtgctt gtgccactta aatggctttc cgattatgtc gatatagatc
     3541 tttgtatcga tgagcttgca gataggctga caatgagcgg gaccaaagtg gagactgtaa
     3601 cagatatcgg ggataggctc aatcgtattg ttgcaggaaa aatagtctct gtaagcccgc
     3661 atgagagtgt cggccgtctt cgcgtggcta ctgtagactt gggcccgcgc caggcgcgga
     3721 gaactctggt aacggccgct gcgaatattg gcaggggtga cattgttcca gtggtcttgg
     3781 cgggcgggac gatcgccgat ggaacggaga taggtgaggt agatttttcc ggagttaggt
     3841 cggagggaat gctctgttca gccaaggaac ttgggatttc ttctgatgga tcaggcattc
     3901 tgcgcttgcc agacgatatc aaacccgggg acgacgtagt ctctgctctg ggattggctg
     3961 acactgtgat agaactggag ataacgccga acaggcctga ctgcctctgc atgatcggga
     4021 tcgcgcgcga ggttgctgct attacaggtg caaccctcaa gatgcccaac accgcactaa
     4081 aagaaggcag tgagcgggca gaagaactta tcagcgtgaa gattgatgcc catgacctgt
     4141 gctctcggta cggagcccgg gtaatacaga aggtttctat aggccagtct ccaatctgga
     4201 tgcaggttcg gctgtcagct gcaggcgtga gaccgatcaa caacgtggtg gatgtgacga
     4261 actacgtcat gctagaggtg aatcaacctc tccatgcgtt cgattacgat cgcatgtcgg
     4321 agcatgccgt catagtcagg cgcgcccgtt ccggcgaggt cattgttaca ctcgatggcg
     4381 aagaacgcag gcttgatccg gagatgctcg ttattgcaga tccaggctca gccttggctg
     4441 tggcgggcgt gatgggcggg ctttccacgg agattaccga ggatacatgc actgtcttgt
     4501 tggaatcggc tacgtttgct cgccagagtg tctggaagac ttctcgaagt ctgaagcttc
     4561 gaactgaggc atcatcacga tacgaaaagg ggttggaccc ggagacggtt cctttggctc
     4621 tggatcgcgc tggccatctg ctcgagacga ttggggcagg tgttgctgca tctggaattg
     4681 tggacgccta tccatcagcg gcagctgtta gggttataag tacggatgcg gccaggatat
     4741 caaggcggct tggcgtggat gtgagctccg atgatgtcgc ggattccctg gggcgcttgg
     4801 gtttctctgt gcgtgtcgag agcgtggcga acaaggccaa gtctgagggc ggctcgcttg
     4861 ttgttgaagt tccaactttc agaggggacg ttcaggaaga gatagatctc gtggaagagg
     4921 tcgcccgaat gtggggctat gataggattc cgacgacatt tcctgcagga gcccacgtcg
     4981 gaggatacac atacgagcaa aaaaaggcca tgcaggcgaa gacgatcctg acgggcgcag
     5041 gcgcgagcga ggcgctcacg atcccattca tgtctgtggc cgatcttgac atgctcaggg
     5101 ttgggcaggg cgatcctttg cgcgatgcta ttcgcattga caaccccatg gtcgaagagc
     5161 aatcgtacat gaggactacg atgttgccgt caatgcttgt ggctattcga accaacatca
     5221 acaggggtaa taggggagta tgcctattcg agctaggcaa gacttacttt ccgtcccaaa
     5281 gcctcaaacg tggagaagag cttcgtagag gcgagaatcc agccgtcgag agacaggttc
     5341 ttgtatgcgg catggctggt gcatataccc cgcgtatctg gcatactcgt gaacgtgact
     5401 acgatttcta cgatgcaaag ggaatgctgg aggcgcttct tgaagggatg ggcgtggacg
     5461 gagcaaccta tgttcagacg gtgcgccctt cattccatcc tgggcggacg gcttccatag
     5521 tatgggaaga tcgggaactt ggcgttatcg gagaggtcca tcccgatgtt tcagaggcgt
     5581 acggcgttgc cggacgcgtc tacatgttcg agctggactt tgcaagaatg ttggaagccg
     5641 tagatga
//
LOCUS       DAOSAI010000129         5623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9090_length_5623_cov_4.036458, whole genome shotgun sequence.
ACCESSION   DAOSAI010000129 DAOSAI010000000
VERSION     DAOSAI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9090_length_5623_cov_4.036458"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLM83_05580"
     CDS             complement(<1..313)
                     /locus_tag="PLM83_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016866178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOK70910.1"
                     /translation="MRALVFKDGLREVDMPIPEITTGQALVRVTLAGICNTDIEVTRG
                     YFAYTGIMGHEFVGVVEDADDPDLIAKRVVGEINVSCGECPTCRGGNPRHCDHVKAMG
                     IN"
     gene            1542..2306
                     /locus_tag="PLM83_05585"
     CDS             1542..2306
                     /locus_tag="PLM83_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOK70911.1"
                     /translation="MDSMTCVPAIELNRVSMTKPVLLGNSLTELDIIRDVSFSVPEGE
                     ILALIGPSGSGKSTLMRMINRMEDPTSGQILLFGSDISGLDVRELRRRVGMVSQTPAL
                     LPGKVSDNIVYGPSLRGEPCNPEEYLELVGLEPELVSRPASSLSVGQQQRMCIARALA
                     NRPNILLLDEPTSALDRTAARNIIELICNLNRQLGLTVIIVTHVMEHAQAVATRVAML
                     VGGVLIEEGAAADFFEGPKTQIGHRFLKGELTEDGR"
     gene            2296..3132
                     /gene="fetB"
                     /locus_tag="PLM83_05590"
     CDS             2296..3132
                     /gene="fetB"
                     /locus_tag="PLM83_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HOK70912.1"
                     /translation="MDANSYIHISNMDLLIALLFIAISGAISHWQKLELERDMAVGTI
                     RAFAQLLAVGFVLQQLFDAARWYWVVLALAIMTTVAAHNGVRRQTGAKQGLFTVMMAA
                     IATGASITLILVIGVVLRVRPWYMPQYVIPIAGMILGNCMNGAALVVNRLHSELTLRR
                     NQVEAALALGAPAKKAASEAMRESLRAAMMPTINSMMTVGLVHLPGMMTGQMISGTSP
                     LDAVRYQIVVMLMIAAATAVTAMTAAFGALGVFFTPFHQLSPRLELGEAWDAYTHDKS
                     KH"
     gene            complement(3374..4417)
                     /locus_tag="PLM83_05595"
     CDS             complement(3374..4417)
                     /locus_tag="PLM83_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOK70913.1"
                     /translation="MGNEKDRKTGLSVEALLAVFIALACILTVFAIANQRRSSRVEIS
                     DLPERVRFAVLCTMDANAGGTKSVAWAPDSQRIVTGEGDGLVVVWNARCGEKSLTLHG
                     RPGYQIENVCWSPDGKHIAAITGEDEFTVWDSGSGEVEWTVVDPEVYGLEGWSEDGKS
                     VTTWNGEEYFSSWNIESKTMENRWFVSIGEQSSVPSPDGKLAAWQVGEDVWAIHTVSS
                     EKFDTKELCGHSGFVRHVSWSPDSSRLAGATDEGIVVWNVETGQLEKEHKRAAGGFAN
                     VLWSPDGRHILAYTDDGSGVILDAEQVDPVADISVDGRRWWSLPVAWSPDGSLLAAIT
                     QDQTVLIYGQITY"
     gene            complement(5069..>5623)
                     /locus_tag="PLM83_05600"
     CDS             complement(5069..>5623)
                     /locus_tag="PLM83_05600"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOK70914.1"
                     /translation="MIPGYNHLSRPLSEMTAQGSATKPALDRWFILLDTMVIGFASVM
                     PASVCTQNDGVATYVQRGAAFLALAGVLGIIMTLWFPMDGSILVTTVVGTLHVFIAAA
                     IMVCWMLAILLFGFGLRNSSEWGGFAFYSFATFAFAVSCSIVTAHAFLLGTDLQGAWQ
                     RLTAGAILQWIFVVSLRALSAPMD"
BASE COUNT         1362 a         1646 c         1352 g         1263 t
ORIGIN      
        1 cgtttatgcc catggctttt acatgatcgc aatggcgcgg gttgccgcca cggcatgtcg
       61 ggcactcgcc gcagctcaca tttatctctc ccaccacacg cttagcaatg aggtcgggat
      121 catcggcatc ctctaccacg cccacgaact catgccccat gatccccgtg taggcaaaat
      181 agccacgcgt aacctctatg tccgtgttgc agattcccgc aagtgtcacg cggaccaagg
      241 cctgccccgt agttatctct ggtatcggca tatccacttc tctaagtcca tccttgaaaa
      301 cgagcgcacg cacagaaggt cctccttcct ccgtatcaac tacaattcat tctatcaccc
      361 gattcaagca ggttgcatcg ccctatcccc gtaagctacc caggagctct gtcaacaatc
      421 tacccccact ttatcttcga tcactgctac tttgttctgc ccatactctc gttcccgcgc
      481 ctttgccgct aatgctgctc aaacatgccc tcaccgcacc ctcatgttct ttgcggatag
      541 tgccccgaag cgccgatccg aacagacgct ttcgcggatt gaataggaca ccgtcgataa
      601 atgcggtcag ccttcagcgc ttgctcaagc actctatttc gatggaccat catgcccagt
      661 ccggcctgta tgcgcgtctg aaacagcttc acaccgcccg gtatgcccat tcggcaaaag
      721 cccagacaga tcgtagagca tgagcgcatt ttcttagttt gagaagcatt aatcgtccag
      781 cagcgtgagc ttgtgctgta taatggaacc gcagtctcat cgtctctgtc gtcttgccac
      841 aagtccaata ggtttcttct catagcaagg ttatgttatc attgctcctc attatggaaa
      901 tagaggtata ggagccgtct ccagcacgaa gggcgcacat ctcaaacgga catactccat
      961 caaccttttc tcactttccg agcaaaacct gcctcgggcc gcggattcca tgcggcatcg
     1021 gggggccgca actgttgaag agcatatggc tgagtaatat aggcagccgt gttcaccata
     1081 ccgttgcagc actcctcgcc ttccttctcg actagctgat cacatagttt gaaaggggtg
     1141 gactctccag ctggatcggt tccatgtgga ttttctcgaa gcatggcctc tccttgactc
     1201 tgggcgaggt gatggtaata cgattcttca caggcgcccc gggagtggac aacaaggtca
     1261 tacctgcaag gctcgtgtcc aaccttgtgg tcatttcgaa ggtcgcgcac ttaagcacaa
     1321 catggtgtag aatctaagag gcaggcctgg ccgccaataa agccctgcct gtctactgct
     1381 gtgttacaaa tggcttgcaa ccgcacagca cggttcggaa gcagagcggc gtaatcgttt
     1441 ggtaaattcg tgttcttccg ccgcaacttc caatccatac agacgttccc tgttgaagtg
     1501 acaaggcttg taaacgatcg aaaacggagg ccacaaagtg aatggattca atgacttgcg
     1561 tacctgcaat tgagctcaac cgcgtaagca tgacaaagcc cgtcctcttg ggcaactcgt
     1621 tgaccgagct tgacatcata cgtgatgtct cgttctctgt gcccgaggga gaaatcctgg
     1681 ccctcatagg cccctcaggg tcggggaaat cgaccctgat gcgaatgatc aacagaatgg
     1741 aagaccctac ttccggccag atcttacttt ttgggtccga catatcagga ttagatgtga
     1801 gggagctcag acgtcgagtt ggaatggtgt cgcaaacccc tgccttgctg cctgggaagg
     1861 tgtccgacaa catagtgtat ggccccagtc tcagaggcga accgtgcaac cccgaagaat
     1921 acttggagct tgtaggcctg gagccagaac tcgtgtcaag gcctgcttcg tctctgtctg
     1981 taggacaaca gcaacggatg tgtatcgcgc gggctctggc caatcgtccc aacatcctgc
     2041 tccttgatga gcctacatca gcgctggacc gcactgcagc gcggaacata atagaactca
     2101 tctgcaacct caaccgtcaa ctggggctca cagtcatcat cgtaacccac gtgatggagc
     2161 acgcccaggc cgtagcgacg cgcgtggcga tgcttgttgg cggtgtcctg atagaagaag
     2221 gcgcagccgc ggattttttt gaaggcccca aaactcaaat tggccacaga tttctcaaag
     2281 gagagctgac agaggatgga cgctaactcg tatattcaca tttccaacat ggatctactc
     2341 attgcacttc tcttcatcgc tatctccgga gcaatttccc actggcagaa gctggagctt
     2401 gaacgcgaca tggctgtagg aaccatcaga gccttcgcgc aattacttgc tgtcgggttc
     2461 gtactgcagc aactctttga cgcagcccgt tggtattggg ttgtgcttgc ccttgccatt
     2521 atgactacag tggcagcaca caacggagta aggcgtcaga ccggcgccaa gcaaggattg
     2581 ttcacagtga tgatggccgc catcgcaaca ggagcatcaa ttaccttgat tctcgtaata
     2641 ggcgtagtcc ttcgggtgcg cccttggtac atgccgcaat acgtgatacc tatagctgga
     2701 atgatactag gtaattgcat gaacggagca gccctagttg taaatagact ccactcggag
     2761 cttaccctca ggcgaaacca agtcgaggcc gcactggcgc tgggcgctcc cgccaaaaaa
     2821 gcggcatctg aggcaatgcg ggaatcgttg cgggcggcaa tgatgccgac aataaactcc
     2881 atgatgactg tcggcttggt gcatcttcca ggaatgatga caggacagat gatttcgggc
     2941 acaagcccgc tagacgcagt ccgataccag atcgtggtga tgctcatgat agccgcggca
     3001 acagctgtaa cagctatgac tgcggcgttc ggcgccctcg gcgttttctt cacgcctttc
     3061 catcagctat cgccaaggct cgaactaggc gaggcatggg acgcctatac acatgacaag
     3121 tccaaacact gacacagcca tggcggaagt taaacatccc tctcaacagt gcgcacgaag
     3181 aacctcacgg caagcctaag tccctgcgtt tagaagacgc tgatctcgta gttcccacac
     3241 ttcgtttcct tgcagcctca atggctgcat gctctttcca gagccctacg aggcctcgaa
     3301 gactatctac cccgccgggc tctggaaaaa gcacagggcc cagagcgaat ccctattaat
     3361 atggcaaggc caatcagtaa gttatctgcc catatatcaa caccgtttgg tcctgggtta
     3421 ttgctgcaag gagagacccg tcaggactcc aagccacagg taaagaccac caacgccttc
     3481 catcaacact gatatctgca accggatcga cttgctcagc gtcgagtatc actccggaac
     3541 cgtcatcggt gtaggccagt atgtggcgcc catccgggct ccacaacaca ttggcaaacc
     3601 cgccggcagc tctcttatgt tccttctcca gttgacccgt ttcgacgttc cataccacga
     3661 tgccctcatc tgtggcaccg gccagacggg aactgtcagg actccaagag acatgacgca
     3721 cgaaacctga atgcccacaa agttcctttg tatcgaactt ttcagacgaa acggtatgga
     3781 tagcccagac atcctcaccc acctgccatg cagcgagttt cccgtcgggg ctgggcacac
     3841 tgctctgctc gcctatgctg acaaaccaac gattctccat ggtttttgac tcgatattcc
     3901 acgaagagaa gtattcctcc ccgttccagg tcgtcaccga cttgccgtcc tcgctccagc
     3961 cttccagtcc atacacctca gggtcaacaa cggtccactc aacttctccg ctccctgaat
     4021 cccagactgt gaactcgtct tcgccagtaa tagccgcgat atgtttcccg tcagggctcc
     4081 agcatacatt ttcgatctgg tagccgggcc ttccgtgaag cgtcaggctc ttctcgccac
     4141 agcgggcatt ccacacgacc acaagcccgt cgccttcgcc agtgacaatt cgctggctat
     4201 caggcgccca agccacgctc ttcgtgccgc ctgcattcgc atccatagtg caaagcactg
     4261 caaagcgaac cctctccgga agatcagaaa tctccaccct ggaagagcgt ctctgattcg
     4321 caatcgcgaa aacagttagt atacaggcca atgctatgaa gactgcaaga agcgcttcga
     4381 cagacaaacc agtcttgcga tccttctcgt tccccatctt gcaacacctc agcttcagtc
     4441 aaaatggagc ccacagcgga gcttccaagg taagtttaca tggccctccg ggaagctgca
     4501 atcgcccccg tcgtcgtcat acaaagcagg aacaaggttc cgatctccgt gattaccgga
     4561 cttgtctcga tggaggctgc aatttagaca gcaatgccca ccaccggctc gaggattgca
     4621 ccagggtctt caatgtcaga cgggtgcatc ttttccatac aggtttctgg caaaagtgta
     4681 tctatcatgt gtcgggcgcg cctcagggaa cctgctttca caaaaaagaa tctgtgtatg
     4741 caaaactttg agactcgcgc cagcttttca cttacacacg tagcctcagg tcatgagcat
     4801 gtgatcctag ccattccaaa ccgggatggc atcaactcga gcctctgcca ctcaatgcaa
     4861 cgttaagccc tcacgcttga cggatcgtta acagaatatc aacccttctt tcgatatatc
     4921 tgcacattac ggctgtacgt ttcgttgcgc aagtttgctg aaagacggat atcagcaatc
     4981 atcatctcaa ttcggtctca gtcacctgct aatggcctat ggatggattc ccctcaaaat
     5041 cgcgctctgg caaagaaagt caccaatgtc agtccattgg cgcagacaac gccctgagag
     5101 ataccacaaa tatccactgc agaatcgcgc cggcggtcag acgctgccat gccccctgca
     5161 aatcagttcc aaggagaaag gcgtgcgcgg tgacaatcga acatgatacc gcaaaagcga
     5221 aggtagcgaa ggagtagaat gcaaagccac cccattcaga actgtttcgc aaaccgaatc
     5281 caaagaggag gatcgccaac atccagcaca ccattatggc ggcggctata aacacatgaa
     5341 gggttccgac gacagtagta acgagaatcg aaccatccat gggaaaccac agtgtcatga
     5401 ttatacctaa aacgcccgca agggcaagga atgccgctcc gcgctgcaca tacgttgcga
     5461 ccccgtcgtt ttgagtacag acggaggctg gcatcacaga tgcaaatccg atcaccatcg
     5521 tgtcgaggag gatgaaccat cgatcgagag caggtttggt tgcgctgccc tgggcagtca
     5581 tctcgcttag cgggcgtgaa aggtgattat agcccgggat cat
//
LOCUS       DAOSAI010000130         5620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9096_length_5620_cov_4.284996, whole genome shotgun sequence.
ACCESSION   DAOSAI010000130 DAOSAI010000000
VERSION     DAOSAI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9096_length_5620_cov_4.284996"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLM83_05605"
     CDS             <1..270
                     /locus_tag="PLM83_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70915.1"
                     /translation="ANAPKERAGYLAKLGSKVDVKSKGFRYSDLIPELFEKKLIIFRS
                     QLDQYGELVLAKPDGPYLIVFDAGPGHVVVKKGPALTSAEKKNFR"
     gene            296..1099
                     /locus_tag="PLM83_05610"
     CDS             296..1099
                     /locus_tag="PLM83_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HOK70916.1"
                     /translation="MARPHKPWNRVVYTLTMALAIIAVYSASSMAHFQMVLPSDDIIE
                     ENESNVVQLQLIFTHPAEASHTMNMDKPIEFGVYHKGKARDLTDTLEAFEFHGAKAWQ
                     TEFTARGAGDFVFYLRPSPYYESMEDSYITQYTKVVVNNAGFPTDWDVEIGLEAEIVP
                     LCKPYGLWTGNVFQGVVKQNGKAVPFAEIEVERLNADAFSSFAGLGQTYPSSAHVTQI
                     IRADGNGVFTYGIPKSGWWGFAALMEGESIDGKDHEIGAVIWVKAYDME"
     gene            1167..2984
                     /locus_tag="PLM83_05615"
     CDS             1167..2984
                     /locus_tag="PLM83_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOK70917.1"
                     /translation="MNLLQLVAEERERLLREEQSEPARLVTTAYYRQASRVFDECAVR
                     EAKQKESLSNRIDRIVLHRFFGPLILAGILYVLYYLSIAKGYQLTNYTWPLLAGFRNW
                     VAQILPPEGILFDPTVRDMVLSVVDGLLAVINYVPIFAILFSLVAILEDVGYVPRMAF
                     IMDRILRQFGLHGQSVFPMVLGGVVVGGCAVPAIMATRGMKDQRARLATILIIPLLNC
                     LAKTPLHILLIEMFYGEHKGFAVFFISTISLIIALTVSKLLSLTVLKVKESAPFVMEM
                     PAYRLPTIRGVFQRCWERLWMFFRKIVSVVLLVMIVVFALTNLPGLNASKKTAYSAEA
                     DKMVDAFLASVDDDSVYAQLLSGNGIVELIDFSNQFDAGKKSARSEAKLDKVHKDFMA
                     KNLEFYKLVNRGRFETNGMIEVDPEAARISRAYRSFERERKALRARASDDIIVHSALG
                     IVGRALEPVTKYAGFDWKVNIALISSFAAKESSVATLGSIYGLDTGVRLEESVAEGDL
                     GWTDLHALALMVFMVVFPPCIPTLLAVKMETGSTMWMLLAAFYPVLLGLLLASLIFTG
                     GNALGLSGLQAMIRFYAISIMLMLVLATVKGPSEDEFDQ"
     gene            3007..3249
                     /locus_tag="PLM83_05620"
     CDS             3007..3249
                     /locus_tag="PLM83_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOK70918.1"
                     /translation="MDKTIMPLTELPRGCKGTVVNVGNYGRGLARRMSDMGLYDGSEV
                     MVISSAGAGPVLMQVNDCTIAVGRGVAKKILVAKEM"
     gene            3256..3501
                     /locus_tag="PLM83_05625"
     CDS             3256..3501
                     /locus_tag="PLM83_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOK70919.1"
                     /translation="MNDAVRLDSLKPGDTCRLHDIKANDATAQRFMDLGLIPGVEIKV
                     VRNAPLVDPVELLVKNNYVSIRHSEARRLEVIPLCRR"
     gene            3489..4157
                     /locus_tag="PLM83_05630"
     CDS             3489..4157
                     /locus_tag="PLM83_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOK70920.1"
                     /translation="MPEIVIAVAGQPNSGKSTIFNMLTGLRQFAANYPGVTVEKRVGR
                     LDYSGHKFTLVDLPGTYSMTSYSLEERLARDFLLNEKPDVILNVVDASNLERNLVLTF
                     QLLEMRMPMVVALNMMDAAENAGIEIDVERLSKKLGVRAVPTVAKKGIGKPELCDALL
                     KAYECRDEANDFRLDYGPQLNRSLEVLEEALAGDSRASGYDPRWLAVRLIAGDNEISR
                     IFAD"
     gene            4335..5516
                     /locus_tag="PLM83_05635"
     CDS             4335..5516
                     /locus_tag="PLM83_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOK70921.1"
                     /translation="MKKRSGIIALLIMTLLALECTAVHAQEYADYPPDGMEPQFLKAR
                     VLSVEKVISDYAVPDDFAVESEVIKLELTSAPYKGVVVQSTSLKSGHPAYDFDVEPND
                     RVIVWAEILDGNIVEAHVFSPDRGHYLKWLVVGFMGVLLLVGGLTGIKTILTLGVTGL
                     AIVWILLPVLLRGYDPIYVTILVSSAITVITLGIVGGFNKKTLTAMIGTTVGVIIAGL
                     IAMIVGSAAHLTGFGTEEATMLFYIPQEVQFDFDRLLFSGMIIGALGAVMDVAMSIAS
                     SMEEVMLARPRITQEQLLRSGLRVGRDVMGTMSNTLILAYAGGSIPLLLLFLAYETPI
                     IQIVNFDHIATEIVKALAGSIGLIAAIPITALVGSRLFKTARPGSAKKPASLQRQKEV
                     I"
BASE COUNT         1442 a         1325 c         1557 g         1296 t
ORIGIN      
        1 gccaacgccc caaaagagag ggccggttat ctagcaaaat taggtagcaa ggttgatgtc
       61 aagtccaaag gattccgcta ttcggacctg attcctgaat tgttcgagaa gaaactgatc
      121 atattccggt cccagctcga ccaatacggc gagctagtcc ttgcaaaacc ggacggacca
      181 tacctgatcg tgttcgacgc cggcccaggc cacgtagttg tcaaaaaggg ccccgcgctc
      241 accagcgcag aaaagaaaaa cttcaggtaa ttgcttagag gaggaagaac aaaaaatggc
      301 ccgaccgcac aaaccatgga atagggtggt atacacgctg acaatggctc tggcgattat
      361 tgcggtatat tccgctagtt ccatggcgca ttttcagatg gttttgcctt cagatgacat
      421 catagaggag aacgaaagca acgtggtcca gctgcagctg atattcaccc atcctgcaga
      481 ggcatcgcac accatgaaca tggataagcc gattgaattt ggagtgtatc acaagggaaa
      541 agcgcgtgat ctcaccgata ctcttgaagc gtttgagttt cacggtgcaa aggcatggca
      601 gaccgaattc acagcgagag gcgccggtga cttcgtattc tacctccggc cgagcccgta
      661 ctatgaaagc atggaggatt cctacataac ccaatacacc aaagtcgtgg taaataacgc
      721 tggattcccc acagactggg atgtcgagat cggtttggag gccgaaattg tgcctctttg
      781 caagccgtac ggcctttgga cgggcaacgt ctttcaagga gtcgtaaagc agaacggcaa
      841 ggcagttccg tttgccgaaa tcgaagtgga gaggctcaac gcggatgcct tcagcagctt
      901 cgcaggctta ggccagacgt atccgtcgtc cgcgcacgtg actcagataa tcagggccga
      961 tggaaacggg gttttcacgt acggaattcc caagtccggc tggtggggat ttgccgccct
     1021 catggaaggt gaaagcattg atggcaagga ccatgagatt ggcgctgtga tctgggtaaa
     1081 agcatatgac atggagtagt tgacgcgagc ttttggggca tccgctatcg cacaggatgc
     1141 cccatccaca cggaaggagg cccatcatga acctattgca gcttgtagct gaagaacggg
     1201 aacggctctt gagggaagag caaagtgaac cggcgaggct ggtgactacg gcatactatc
     1261 gccaggcctc aagggtattt gacgagtgtg cagtcagaga ggcaaagcag aaggagagcc
     1321 tcagcaacag aatagaccgc atcgtgctgc accgtttctt cggtcccctg atcctagctg
     1381 gaatactgta cgttctctat taccttagca ttgctaaagg gtatcaattg accaactaca
     1441 cctggccttt gcttgccggg tttcggaatt gggtagcaca gatactgccc ccggagggaa
     1501 tactgtttga cccgaccgtc agggacatgg tcctgagtgt cgttgatgga ctcctggctg
     1561 ttatcaatta tgttccaata tttgcaatac ttttcagtct tgtagcgatc ttggaggatg
     1621 tcggttacgt gccgaggatg gcctttatta tggacagaat acttcgccag ttcggactac
     1681 acggacaatc tgtgtttccc atggttttgg ggggcgtagt agttggagga tgcgctgtcc
     1741 ctgccatcat ggctacgaga ggaatgaagg atcagcgggc acgtctcgcc accattctta
     1801 tcattcccct tctgaactgc ctggcgaaga ctcctctgca catactcctg atagagatgt
     1861 tctacggaga gcacaagggc tttgcggtct tcttcatatc caccatcagc ttgataatcg
     1921 cgttgacagt gagcaaactt ctgtctctga cggtattgaa agtcaaggaa tcggctccgt
     1981 tcgtaatgga gatgcctgcc tatcgcctgc ctaccatcag aggagtcttc caacgctgtt
     2041 gggaacggtt gtggatgttc tttagaaaaa tcgtatccgt ggttcttctg gtcatgatcg
     2101 ttgtattcgc ccttaccaac ttaccaggct tgaacgcaag caagaagacg gcctattccg
     2161 cagaagcaga caagatggtc gatgcttttc tcgcgtcagt ggacgacgat tccgtgtacg
     2221 cccagctcct atcgggtaat gggatagtag agttgatcga tttcagcaat caattcgatg
     2281 ccggaaagaa atctgcaagg agcgaagcca agctcgataa agtgcacaag gatttcatgg
     2341 ccaagaattt ggagttctat aagttagtga acagaggaag attcgaaacg aatgggatga
     2401 tcgaagtgga tccggaagcc gccagaatat caagggctta cagatccttt gaaagagagc
     2461 gaaaagccct aagagctcgc gcaagtgatg acatcatcgt ccacagcgct ttgggcatag
     2521 tcggcagggc gctcgaaccg gtcaccaagt atgcagggtt cgactggaaa gtcaacatag
     2581 cactgatcag ttcatttgca gcgaaagaga gcagcgtcgc aactttggga agtatctatg
     2641 gactcgacac cggggtcagg cttgaggaga gcgtcgctga aggcgatctt ggctggaccg
     2701 acttgcatgc actcgctttg atggtcttca tggtggtctt cccgccttgc ataccaacat
     2761 tgttggcagt aaagatggag acgggaagta cgatgtggat gctgcttgca gcattctatc
     2821 ccgtgctgct cggcttgttg ctggccagtc tcatattcac cggcgggaac gcgctcggtc
     2881 tgtcaggctt acaggccatg atcagatttt acgcgatctc gatcatgttg atgctggttt
     2941 tggcaacggt taaggggcca agcgaagacg agttcgatca atagatagtt gcaggaggga
     3001 gtttgcgtgg ataaaacgat tatgccattg accgagcttc ctagagggtg caagggaaca
     3061 gtggttaatg tggggaatta tggcagaggc cttgctagac gaatgtcaga catgggcctg
     3121 tacgatggat ccgaagtgat ggtgataagc tcggcaggag caggtccagt attaatgcag
     3181 gtaaacgact gcacgatcgc agtgggcaga ggagtggcga aaaaaatact tgtagctaag
     3241 gagatgtgat gatcgatgaa tgacgctgta cgactggata gcctgaaacc gggcgatacg
     3301 tgccgtcttc acgacataaa ggccaatgat gcaactgcac agcggttcat ggatttaggc
     3361 cttattcccg gagtcgaaat caaggtggtg cgaaatgcgc ccctggtcga cccagttgaa
     3421 ttgctcgtca agaacaacta cgtgagcatc cgccactcag aggcgagaag actcgaggtg
     3481 attccgctat gccggagata gttatagcag tagccggtca accgaactca ggaaagtcca
     3541 ctatattcaa catgttgact ggcttgcgcc aatttgctgc aaattacccg ggggtaacag
     3601 tggagaaacg tgtaggccga ttggactata gcggtcacaa gttcacgctg gtggacctcc
     3661 ccggtacata cagcatgact tcctacagtt tggaagagag gcttgcccgg gatttcctgc
     3721 tcaacgaaaa accggatgtg atcctaaatg ttgtagacgc ctcaaatctt gagcgcaacc
     3781 tcgtactcac ttttcagctc ctcgagatga ggatgccaat ggttgtggcg ctgaacatga
     3841 tggatgcagc cgaaaacgca ggcatagaga tagacgtcga acgcctcagc aagaagttgg
     3901 gggtaagagc ggtcccaact gtggccaaaa agggcatagg aaaacccgaa ctatgcgatg
     3961 ccttgctaaa agcctacgaa tgcagggatg aggccaacga ctttcgcctg gactatggcc
     4021 cacagctcaa ccgatctctt gaagtgctgg aagaagctct ggccggcgac agtagggcat
     4081 ccggttacga cccgcgctgg ctggccgttc gattgatagc aggagacaat gaaatcagca
     4141 ggatatttgc tgattagcac agaatatgga tttacttgaa aggagggtaa ggcattgagc
     4201 ggtaccgcaa tcatcgcagc tagcggggca ttggcagcat acttgcttat tcggaaattg
     4261 aaaaagcccc ctaggtgtaa aaaatcaggc cgatgcagcg gatgcgcttc ttcctgcgat
     4321 tgggggaaac acggttgaaa aagagatcag gtattattgc tttgctcata atgacgctgc
     4381 ttgcgctgga gtgcactgcg gtccatgcgc aagaatacgc cgactaccct ccggatggaa
     4441 tggagcctca gtttttgaag gccagagtgc tcagtgtcga aaaagtcatc agtgattacg
     4501 ccgttcctga cgattttgca gtggaatcgg aggtcatcaa gctcgagctc acatcagcac
     4561 catacaaagg cgtagtagtt cagtcgactt ctctgaaatc agggcaccct gcttacgact
     4621 tcgatgttga accaaacgat agagtgatag tctgggccga gatcctcgac ggcaacatag
     4681 ttgaagccca cgttttctct ccagatcgcg gccactatct caaatggctc gtggtcggat
     4741 tcatgggcgt gctgcttttg gtcggcgggc ttacgggcat caaaaccatc ttaacactgg
     4801 gagtaacagg gctcgcgata gtatggattc tgcttcctgt tttgctcagg gggtatgatc
     4861 cgatttacgt cacaatcctc gtatcaagcg ccatcaccgt cattactctc ggtatcgtag
     4921 gaggttttaa caaaaagacg ttgacggcca tgatagggac cacggtggga gtgatcattg
     4981 ccggactgat cgcgatgatc gtgggctcag cagcgcacct caccggcttc ggaacagaag
     5041 aagcaaccat gctcttttac ataccccaag aagtccagtt cgattttgac cggcttctgt
     5101 tttctggaat gatcataggc gctctgggcg cagtaatgga cgtagcgatg tccattgcat
     5161 cgtcgatgga agaggtcatg ctggcaaggc cgcgaatcac gcaggagcag ttgcttcgct
     5221 cggggttaag ggtagggcga gatgtaatgg gcacgatgtc caacacccta atactggcat
     5281 atgcaggggg ttccatcccg ctcttattgc tctttttggc gtatgaaact ccaataatcc
     5341 agatcgtcaa cttcgaccat atcgccaccg aaatagttaa ggcacttgcc ggaagcatcg
     5401 gcctcattgc cgccattccc attacagcac ttgtgggaag ccgtttgttc aaaacggcta
     5461 ggccaggatc tgcaaaaaaa ccggcgtctt tgcagagaca gaaagaagta atatagcata
     5521 ttgtgtcgac atcataagta aacgcgatag ataacgggat ggttcgtcgc gctgaaccgc
     5581 ggtagcgtgc gacagtcatt tatagaccta ccgagcaccc
//
LOCUS       DAOSAI010000131         5588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9164_length_5588_cov_3.960419, whole genome shotgun sequence.
ACCESSION   DAOSAI010000131 DAOSAI010000000
VERSION     DAOSAI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9164_length_5588_cov_3.960419"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(417..1550)
                     /locus_tag="PLM83_05640"
     CDS             complement(417..1550)
                     /locus_tag="PLM83_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOK70922.1"
                     /translation="MMSNGFMGKWGGELNHLCKNWAIPVESSGKIQKGGAEVQIEITK
                     SSDLLDSNGVLIQRGWAKDLLLRYNRERIRASKLKIKEWDYYCILTEEYGAAFTIADL
                     GYAGMGSVTFLDLTEPGEITQTVITGFPMGKLNMPTTSHEGNVSFEHKNLNLEFIKDG
                     RQRLINVFFANFADGKPLQGTIRMTQPKPMDTMVIATPFAGNPHAFYYNQKINCMPAE
                     GILMLGSEKRAFDPGRSFAVLDWGRGVWTYSNTWYWGSASGLVDGTPFGFNIGYGFGD
                     TSCASENMLFYNNKAHKLDQVQFHIPPDDYLAPWKFTSNDRRFEMDFYPIIDRNADIN
                     ALFFRSNQHQVFGRFTGRAVLDDGTVIELKDFLGFAEEVKNRW"
     regulatory      1729..1915
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2053..4281
                     /gene="cobU"
                     /locus_tag="PLM83_05645"
     CDS             2053..4281
                     /gene="cobU"
                     /locus_tag="PLM83_05645"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOK70923.1"
                     /translation="MGTSVSANAIGFAYGLAPVFSDVSFRLDPGRFLGVVGPNGAGKT
                     TLLKCLAGLLKPSAGEILLGGGCLSCLQREEIAKIVAVVPQDTRVGFDFSVEEFVMMG
                     RIPHLKRFSRETRKDREAVRWAIESTGMAPFAQRLVTSLSGGERQRAAIARALAQEPK
                     VLLLDEPTSHLDIRRQVEILDLVKIMSLERDITVVGVFHDLSLASMYCDEILMMTEGE
                     ARYSGTPEEVLTQENVGSLYGDKVVVTDHPVHSTPLVAPRSCALLNKDNEPGYVTGIE
                     NKPCEAGELGKSDRPDKSGRLTLITGGARSGKSSFAERMAAEAGTKVMYVATCEPMDE
                     EMRARVSRHRERRPKSWITVEEPLDVAATIANEASNYDAVLVDCLGLFVSNHLFAQDE
                     EADDQSKTEAVLAEVARFAEAARDAGSHVIVVTNEVGMGVVPAYPLGRLFRDVLGWAN
                     QIVAEEADEVYLCVAGEPVRIKPRGEAGVGERLRAVNRSESPRPRLERIRDLLIYNMP
                     DGAALVVSCDSAGAIGPKGRDVIATSGYVLGRYITRVALMELIACGAQPILVVDALCV
                     ERDPTGDEIIKGVRDEAALAGLDPEMAVTGSTEENIATSQTGIGIAAIGIAERSELRL
                     GRASVGDALVCIGVAKVGNEVTLDDPEIVDLGAVRALANIEWVHEIVPVGSRGIRAEA
                     EDLAETNGLRFRVKPGAHLDLDKSAGPATCVVAACDDAHWQELSAMIGKPVFMVGEVE
                     AP"
     gene            4278..5030
                     /gene="cobS"
                     /locus_tag="PLM83_05650"
     CDS             4278..5030
                     /gene="cobS"
                     /locus_tag="PLM83_05650"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOK70924.1"
                     /translation="MKRVIDRFAIAMSFLTVLPVYRGDAPWEDEIARSAVFFPVIGLV
                     IGLIVAAVDALALHAFSPLCAAVIGMMVQFWVTGGMHVDGLMDTADGMLSGRSADRIL
                     AIMHDGSVGPMGATVGVFIIILKVALAASMSGRTRTAALMCAPAMGRLSMVLSFCLFP
                     PATPERGMGRQLARKVGPGEFAITLSCTAALCFMLAGPRSLSAAACSLGVALFAGWRV
                     SSSIGGVTGDVYGALCEIAEVVSLAAFAFTAA"
     gene            5058..>5588
                     /locus_tag="PLM83_05655"
     CDS             5058..>5588
                     /locus_tag="PLM83_05655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOK70925.1"
                     /translation="MVKRIYLVRHGETAATSGRRFEGSGPISGLGTPLSEKGAIQAEM
                     AADALAGEDVFGVYASPLLRTVQTATPISQATGVAIELEPDLAEGSFGAWEGMTFDEI
                     EHRYPLEVKRWFADPLGYAPPGGESVRQLAARVIPCIERIAGKSYGEEGTAVVVTHGG
                     PIRAVLSSCLCGNLEMF"
BASE COUNT         1172 a         1427 c         1718 g         1271 t
ORIGIN      
        1 gatctatttt gggatgagcc gctttcaatg gccctatcat gaagcatacc aggcagagat
       61 aagtttcaaa gacgagatca tacctggaag gttgcatatg cacagaagtc ctcgaattct
      121 ttgcccgttt atagaacacg gagtgaactg aagagcagca cccgcacaat gctggatttg
      181 gcctacgaat aggatgctca tggaaacgtt gcgaaacagc ctgcaaatag tgtttgaaga
      241 ccattaggcg gccaacgtct cactcatggc tatcgccgtc ggggccggcc aagttcataa
      301 tgacttgctc gggccgtctt gtctagttgg gacacgcgaa acaggggctg tgcgtggccg
      361 tcaaccacat agtaggatag cgcgtatggg ccgccgccca cgggggatgc cctctgttac
      421 catctgttct tgacttcctc cgcaaaccca aggaagtcct tgagctcgat gacggtgcca
      481 tcgtccagca ccgctcttcc cgtgaacctg ccaaagactt gatgttgatt cgagcggaag
      541 aacaacgcgt tgatgtctgc attccgatcg atgatggggt agaaatccat ttcgaatcgc
      601 cggtcattgc tcgtgaattt ccagggcgca aggtaatcgt cgggaggaat gtgaaattgg
      661 acttgatcga gtttgtgggc cttgttgttg tagaataaca tgttttcgga cgcgcatgat
      721 gtgtctccga atccatagcc tatgttgaaa ccgaacggcg tcccatctac aagccccgag
      781 gctgagcccc agtaccacgt attcgagtac gtccatactc cccttcccca atccaagaca
      841 gcgaaggatc tccccggatc gaaggccctc ttttcgctgc cgagcatgag gatcccttcg
      901 gcaggcatgc aatttatctt ctgattatag taaaaagcat gagggttgcc ggcaaatggc
      961 gtggctatta ccatcgtgtc catgggcttt ggctgagtca tgcgaatcgt tccctgaagc
     1021 ggtttgccat cggcgaagtt ggcgaagaac acgttgatca gcctttggcg accgtcttta
     1081 atgaactcaa ggttaagatt cttgtgttcg aaagatacat tgccctcatg cgacgttgtg
     1141 ggcatgttga gcttgcccat gggaaaaccg gtgatcacag tctgcgtgat ctcacccggc
     1201 tcggtaaggt cgagaaaggt cacagaaccc atgcctgcat agcctaaatc cgcaattgtg
     1261 aatgccgccc catactcctc cgtcaggatg cagtagtaat cccattcctt gatcttcagc
     1321 ttcgaggctc gtattcgctc gcggttgtaa cgtagcaaga gatccttggc ccaacccctt
     1381 tggatcaaga ccccattgga gtccaacaag tcggaacttt tcgtaatctc tatctgcacc
     1441 tcggcgcccc ctttctgtat cttgccactg ctttcgactg ggatggccca attcttacag
     1501 aggtggttca attctccccc ccattttccc atgaaaccat tcgacatcat tgcctgcgtt
     1561 ctcctgccta agcgaggtca aggcacagaa atttatcatg cgttgtaccg gcccacgctc
     1621 agcttgcacc atgggtgggc ttgccggatc cctactttag cggcatgtgc ggatggaagg
     1681 acttcacttg cttccaaggc tcaactcggg tatagttttt gttgacaact gaatcgactt
     1741 caaccaggtg cccatagagg gataataggg aatcaggtgc aagtcctgaa cggtcccgcc
     1801 actgtaaccg gtgagcgcgc gacatgagcc actgtcacga gatgagatgg gaaggctgtc
     1861 gccaacacgc tgtgacccgg aagtcaggag acctgcctgg gttggaagta ccgtgagcct
     1921 tcggcgcaag ggcgggtact gcatgtgaca atccagtcat gtaagtggtt ttagcccgac
     1981 ctttatcgaa aggtcgggtt ttttgatacg acctggtggg cacttgtggc atgatgaggg
     2041 gatgtgaggc agttgggcac gagtgtcagt gccaatgcca ttggatttgc atacggttta
     2101 gccccggtct tttccgacgt atccttccga ttggatccgg ggcgctttct gggagtagtc
     2161 ggtcccaacg gcgcaggcaa gactacactg ctcaaatgtt tggccggcct tttgaaacca
     2221 tcggccggcg aaatcctgct cgggggcggc tgcctctcgt gcttacagag ggaagagata
     2281 gcgaagatcg ttgcggtggt tccccaggat acacgggtcg gattcgactt ttcggtggag
     2341 gagttcgtga tgatggggcg gatccctcac ctcaagaggt tctcgcggga aacgcgcaaa
     2401 gatcgggagg cagtgcgctg ggcgattgaa tcgaccggca tggccccttt tgctcaaaga
     2461 ctcgtcacat cactgagcgg tggagagagg cagcgtgccg ccatcgctcg ggcgctcgct
     2521 caggagccta aagtcctcct tctagatgag cccacatccc acctggacat aaggcgacaa
     2581 gtggagatct tggacctggt caagatcatg agcctcgaac gcgacattac cgtggtcggg
     2641 gtatttcatg acctgagcct tgcatccatg tactgtgatg agattctgat gatgacagaa
     2701 ggcgaagccc gctattctgg cacgcctgag gaggttttga cgcaggaaaa cgtggggagt
     2761 ctgtatggag ataaagttgt ggtgactgat caccctgtgc actctactcc gctcgtagcg
     2821 ccgcgctcct gtgccctgct gaacaaggac aacgagcctg gctacgtaac tggcatcgag
     2881 aacaagcctt gcgaggcggg cgaactcggc aaatcagacc ggccggacaa gtctggacgg
     2941 ctcaccctca ttacaggggg cgcaaggagc gggaagagct catttgccga gcgcatggct
     3001 gctgaggctg ggactaaggt catgtatgta gcgacatgcg agcccatgga cgaagagatg
     3061 cgcgctcggg tgagccggca tcgtgagcga aggccgaagt cgtggattac ggtggaggag
     3121 cctcttgatg ttgctgcgac aatcgcgaac gaggcgagta actacgatgc ggtgctagtc
     3181 gattgcctcg gcctttttgt cagcaaccac ctgtttgcgc aggacgaaga ggctgatgat
     3241 caatccaaga cagaggccgt gctggccgag gttgcaagat ttgccgaggc ggcacgcgat
     3301 gccggttccc acgtcattgt cgttaccaac gaagttggga tgggcgttgt gcctgcatat
     3361 cccctcgggc gtctcttcag ggatgtgctc ggatgggcca accaaatcgt ggctgaagag
     3421 gcagacgagg tctatctgtg cgtggcgggg gagcccgtca ggataaaacc tcggggtgaa
     3481 gcaggagttg gtgaacgatt gcgggcagtc aaccgatccg agtctccaag gcctcgcctt
     3541 gaaagaataa gggacctcct catatacaac atgcccgatg gggcggcgct tgtcgtgtcg
     3601 tgtgactcgg caggtgccat cggccccaaa gggcgcgatg tgattgcaac gtcaggctac
     3661 gtactgggca ggtacatcac tcgcgttgct ctcatggaac ttatcgcatg cggcgcacaa
     3721 cctatcctgg tggtggacgc gctctgcgtc gagcgtgacc ccacaggcga tgagatcata
     3781 aagggcgtgc gggacgaggc ggcccttgcg gggctcgatc ctgaaatggc tgtgacgggg
     3841 agcactgaag agaacatcgc tacctctcag acaggcatcg gcatcgctgc cattgggatt
     3901 gcggaaaggt cagaactgag gctgggaagg gcctcggttg gcgatgccct cgtctgcatt
     3961 ggggttgcga aggtaggcaa cgaagtgact ttggatgacc cggaaatcgt ggatcttgga
     4021 gcagtgaggg ctctggccaa tatcgagtgg gttcacgaga tcgtgcctgt aggttcccgt
     4081 ggaattcgcg ccgaggcgga ggacttggcc gaaacgaacg gcctcaggtt tcgggtgaaa
     4141 ccaggcgccc atctcgactt ggacaagtcg gccggacctg caacgtgtgt ggttgcggca
     4201 tgcgatgatg cacattggca ggagctatcg gccatgattg gaaagccggt attcatggtc
     4261 ggagaggttg aggcgccatg aaaagagtaa ttgataggtt cgcgatagcc atgtcgtttc
     4321 tgaccgtctt gcctgtttac agaggggatg cgccgtggga agacgagatt gcccgctccg
     4381 cagtcttctt ccccgtgatc gggttggtga taggtctcat cgtagctgcg gttgacgcac
     4441 ttgccctgca tgcattcagt ccgctgtgtg ctgcggttat cgggatgatg gtgcagtttt
     4501 gggttacagg cggaatgcac gtggatgggc tcatggacac tgccgatggg atgctgagcg
     4561 ggcgctcagc tgacaggatc ctcgccatca tgcacgacgg cagcgtgggg ccgatggggg
     4621 cgaccgttgg agtgttcata atcatcctca aagttgcatt ggcggcttcg atgagcggca
     4681 gaactcgcac agctgccctc atgtgcgcac ctgccatggg acggttgagc atggttctgt
     4741 ccttttgctt gttccctccc gccacgcctg agcgtggaat gggaaggcag ttggcccgga
     4801 aagttggccc gggagagttt gcgattaccc tgtcatgcac tgccgcactc tgctttatgt
     4861 tagccgggcc cagatcgctt tcggccgcag cctgctcctt aggtgtagcg ctctttgcgg
     4921 gctggagagt atcgagttcc atcgggggag tgaccgggga tgtttacggg gccctatgcg
     4981 aaatcgcaga agtcgtgtcg cttgcggcct tcgctttcac ggccgcatag ctagaggatt
     5041 ggagtgctga gcgcgctatg gtaaaacgta tctaccttgt tcggcacgga gagactgctg
     5101 caacctctgg gcgaaggttc gaaggttcgg gcccaataag cgggctaggg actcctcttt
     5161 cggagaaggg cgcgatccag gctgagatgg cggctgatgc cctggcgggc gaggacgttt
     5221 ttggggtgta tgcgagccct ctcttgagaa cggtacagac cgcaacccca atatcccagg
     5281 ccacaggggt tgcgattgag cttgaaccgg atcttgcgga aggctctttc ggggcatggg
     5341 aagggatgac cttcgatgag atagagcacc ggtatccttt ggaagtaaag cggtggtttg
     5401 cagatccatt ggggtacgcg ccccccgggg gcgagtccgt tcggcagctt gctgcgagag
     5461 tcatcccttg catagagcgg attgccggca agtcctatgg cgaagagggc acggcagtag
     5521 ttgtgacaca tgggggcccc atcagggcag tcctttcgtc ttgtttgtgt ggaaaccttg
     5581 agatgttc
//
LOCUS       DAOSAI010000132         5580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9183_length_5580_cov_3.909864, whole genome shotgun sequence.
ACCESSION   DAOSAI010000132 DAOSAI010000000
VERSION     DAOSAI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9183_length_5580_cov_3.909864"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLM83_05660"
     CDS             complement(<1..260)
                     /locus_tag="PLM83_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOK70926.1"
                     /translation="MEIDILTIFPGMFYGPLSESILGRAQEKGILTVRIHDIRDFSTS
                     RHRVVDDYPFGGGPGMVMKAEPIAAAVEHALAARVQAKESRS"
     gene            complement(331..855)
                     /gene="rimM"
                     /locus_tag="PLM83_05665"
     CDS             complement(331..855)
                     /gene="rimM"
                     /locus_tag="PLM83_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOK70927.1"
                     /translation="MTRSGDQLVLIGVIMGTHGLWGELRVRPENGDPRRFCGIDRVFL
                     GRENCADFQERKVISERAHGEASLVLLEGVEDVETGKLLKGFGLYLPVEELPELPEDE
                     FYAFDLVGLGVYAKTGERLGIVKEVIELPANDVFVVKPDSGREFLVPAVGDIVCEIDV
                     AERRMIIDDRPGLR"
     gene            complement(852..1112)
                     /locus_tag="PLM83_05670"
     CDS             complement(852..1112)
                     /locus_tag="PLM83_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOK70928.1"
                     /translation="MAGNKKNEGRLCQLVTFLAKNLVDDPEEVRVREIESGDSVIFEV
                     SVAPDDMGKVIGRHGRVARAIRSVTKAAAIRDGKRVMVEIVD"
     gene            complement(1125..1439)
                     /gene="rpsP"
                     /locus_tag="PLM83_05675"
     CDS             complement(1125..1439)
                     /gene="rpsP"
                     /locus_tag="PLM83_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOK70929.1"
                     /translation="MAVRIRLTRTGAKKQPHYRLVVADSRSPRDGRFIEILGHYNPRM
                     DPPELVVDVDRAAEWIRRGAQPSETARALLVKAGLDMSGASGKGTQSAKADVAPVDAA
                     EN"
     gene            complement(1503..2840)
                     /gene="ffh"
                     /locus_tag="PLM83_05680"
     CDS             complement(1503..2840)
                     /gene="ffh"
                     /locus_tag="PLM83_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOK70930.1"
                     /translation="MFENLSARLQDVFGNLRNRGRLTEKDVDAALREVRMALLEADVN
                     FKVARDFTARVRERAVGEEVMKSLTPAQQVIKIVMSELTELMGGTAAKISFAPRPPTV
                     IMLVGLQGSGKTTSCAKLANLLRKQGHKPLLVAGDVYRPAAIKQLQVLGGQLGIEVFS
                     MGDKTDPVDIAAAAVERGKSMGYDVVILDTAGRLHVDDEMMEELGRIKSRVNPTEILL
                     VVDAMTGQDAVNVASMFNERLGIDGIIMTKLDGDARGGAALSVRAVTGKPIKFVTTGE
                     KLDAVEQFHPERMASRILGMGDVLTIIEKAQDAFDAEQAMKMEEKLRKQEFTLEDFLS
                     QLQDVRKMGPIDQLLGMIPGLGKSKALKGVAIDEKDLARVEAIVRSMTPAERRNPGII
                     DGSRKRRIAAGSGVRVQDVNALLKQFEQTRKMLKQFGDAAKRGGPPARMPFMP"
     gene            complement(2858..3325)
                     /locus_tag="PLM83_05685"
     CDS             complement(2858..3325)
                     /locus_tag="PLM83_05685"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HOK70931.1"
                     /translation="MLDKMLRMGALLDVHGPLLTEHQREMCVLYYLNDYSLAEISELY
                     GVSRQAVHDTLARAERAMEGHEETFGLLRMAEERRKRVGELDSQLSDADFQLEMAMAS
                     LDEAERSAQRPSGPDLAGVRRNIEQTMEIIASARRLVASIDSGDGGSPGMEDD"
     gene            complement(3713..4669)
                     /gene="ftsY"
                     /locus_tag="PLM83_05690"
     CDS             complement(3713..4669)
                     /gene="ftsY"
                     /locus_tag="PLM83_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOK70932.1"
                     /translation="MSPSFFSNLWSGLAKTRDAIIDPIEALFSKKKVIDEETLEELEA
                     VLIQADLGVAATTAIINKMRESGSRGEQVDADGAVALIEQELISILGERTAPLARADE
                     PPTVVMVVGVNGTGKTTSIGKIASMLAHEGNRVTLAAADTFRAAAIEQLEVWGNRTGC
                     NVVRHQEGSDPAAVAFDAYQAARARGSDYLLVDTAGRLHTKSNLMEELKKIKRVISRE
                     CDEAPHEVLLVVDATTGQNAIAQARVFNEAVGVTGVVLTKLDGTARGGIVVAISQELN
                     IPVKYVGVGEGAEDLRPFSPERFVQGLLRADAGDDGRKGAKR"
     gene            complement(4688..>5580)
                     /locus_tag="PLM83_05695"
     CDS             complement(4688..>5580)
                     /locus_tag="PLM83_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK70933.1"
                     /translation="LLDEGREERAKAAADVNEGELAARVARKRHQEVAEKLNGARIEG
                     ARIAAEYEACVERLASAYSISEEEGLARDVAIQSRAGVQAEIAQLRAEITEIGPVNHT
                     AIEESRKLAERHGFLETQLKDLEAAQASLDEVVRECELTCEKRFLETFEAVRSEFADI
                     FTDIFGGGTADLVLDDPDNPLECGIDIVCQPPGKKLSSLSLMSRGERSLVAIALLFAI
                     MRVNPSPVCLLDEIDSALDEANLIRFAQLLKTTSEDVQVIIVTHRKRTMECADALFGV
                     TMEESGVSKVFSIRTDQQVS"
BASE COUNT         1205 a         1770 c         1286 g         1319 t
ORIGIN      
        1 cttgaccggc tctccttggc ctgcacgcgt gcagcaaggg catgttcgac agctgcagca
       61 atcggctccg ctttcatgac cattccaggt ccaccgccga acgggtaatc gtctacaaca
      121 cggtgccgac tggtagaaaa atcgcgaatg tcgtgaattc ggacagtgag aatccccttt
      181 tcctgcgcgc gccctaggat gctttcagac agtggcccat agaacatccc cggaaaaatc
      241 gtcaggatat cgatttccac gttccgaatc acctacccat gctcaatcca tcccgcatcg
      301 acaagcgccg atgcaagatt actattggca tcagcgcaaa ccaggcctat cgtctatgat
      361 catacgcctt tctgcgacat cgatctcgca gacaatgtcc ccaaccgccg gcaccaagaa
      421 ttcccgcccc gaatcaggct ttaccacgaa aacatcattg gccggcaatt cgattacttc
      481 cttcacgatg cccaggcgtt ctcctgtctt agcatagacg ccgaggccga ccagatcaaa
      541 tgcgtagaat tcgtcttcag gcaactcagg cagttcctcc acgggcagat aaaggccgaa
      601 acctttaagc agttttccgg tttccacatc ctccacgcct tcgaggagca ccagcgaagc
      661 ctcgccatgg gcgcgctccg atatcacttt gcgctcctga aaatcggcac agttctcgcg
      721 tccgaggaaa acgcgatcaa tgccacagaa acgtctgggg tcgccatttt cggggcggac
      781 tctcagctca ccccaaagtc cgtgcgtccc catgattacc cctatgagca ctagctgatc
      841 gccacttcga gtcaatctac gatttccacc atcacacgct tgccatctct aatggcagcg
      901 gcctttgtaa cagaccggat tgcacgtgcc acacgcccgt gtcttccaat taccttgccc
      961 atgtcgtcgg gagccacaga tacctcgaat atgactgaat caccgctttc aatctcgcga
     1021 acgcgcactt cttcaggatc atctacaagg ttttttgcga gaaatgtcac gagttgacac
     1081 agtctgcctt cgtttttctt gtttcctgcc atctctatca ccggttagtt ctcagcagca
     1141 tcgaccggtg ccacgtcggc ctttgcagac tgagttcctt ttccggatgc tccagacatg
     1201 tcgagacctg ccttgaccag taacgcgcga gcggtctcag aaggctgagc tcctcgtcgt
     1261 atccactccg cagccctgtc tacatcgaca acgagctccg gcggatccat tctgggattg
     1321 taatgcccca aaatctcgat gaatcttcca tcccttggcg atcttgaatc agccactacc
     1381 aacctgtaat gcggttgctt ttttgctccc gtcctagtta gcctgatacg aacagccatt
     1441 tccttatact gcacctccga atggttacta ctgaacaaaa agcctaaacc gaactgacgc
     1501 ccctatggca tgaacggcat ccgcgctggc ggcccgccac gtttagccgc atctccgaac
     1561 tgtttcagca ttttgcgagt ctgctcaaac tgcttgagga gagcattgac gtcctgaacc
     1621 ctgactccgc ttccagctgc gatcctccgc tttcgagacc catctatgat gccaggattg
     1681 cgcctttccg cgggagtcat cgatcttacg atcgcctcca cccgcgcaag atccttttcg
     1741 tcgatggcta cgcccttgag cgccttcgat ttccccagcc cgggtatcat tcccaataat
     1801 tgatcgattg gtcccatttt ccgaacgtcc tgaagctgag aaaggaaatc ttcgagtgtg
     1861 aattcctgct tgcgaagttt ttcttccatc ttcatggcct gttcagcatc gaaagcgtct
     1921 tgggcctttt ctattatcgt aagcacatcg cccatgccaa ggatgcgcga ggccatccgc
     1981 tccggatgga attgctcgac tgcgtcaagc ttctctcctg tggttacgaa cttgatgggt
     2041 tttcccgtaa ctgcacggac agaaagcgcc gcaccgcccc gagcatctcc atccagtttc
     2101 gtcatgatga taccgtcaat tcccaggcgc tcgttgaaca tggaggccac attgaccgcg
     2161 tcctgaccgg tcatggcgtc aaccaccagc aggatttctg tggggttgac tctggacttg
     2221 attctcccaa gttcctccat catctcatcg tcaacatgaa gcctgccggc tgtgtcgagt
     2281 attacgacgt cgtatcccat ggactttccg cgttcaacag ccgcagccgc aatgtcaacc
     2341 gggtcagtct tgtcacccat tgagaaaact tcgatcccaa gttgacctcc gagcacctga
     2401 agctgtttga ttgcggccgg tctatacaca tcgcctgcca cgagcaaagg cttgtgcccc
     2461 tgcttgcgca ggaggtttgc cagctttgcg cagctagttg tcttgccgga tccctgcagg
     2521 cctacaagca tgatcactgt cggaggacgg ggcgcaaaac tgatctttgc cgccgtgcct
     2581 cccataagtt cagtgagctc ggacatgacg atcttgatga cctgttgagc cggagtgagg
     2641 ctcttcatga cttcctctcc gaccgcgcgt tcccgcaccc tagcggtgaa atcacgggcc
     2701 accttgaagt tgacgtctgc ctcgagcaaa gccatccgaa cttcacggag agctgcatcg
     2761 acatcctttt cagttagtcg cccgcgattc cgcaggttac cgaaaacgtc ctggagacgg
     2821 gctgagaggt tctcgaacat tatgccttgc ccctcctcta gtcatcttcc atgccagggc
     2881 tgccgccatc tcctgaatcg atagatgcca ccagccgccg ggccgaagca atgatctcca
     2941 tagtctgctc gatgttccgg cgcacccctg ccaaatcagg accgcttgga cgctgggcac
     3001 tgcgctcggc ttcatcgagg cttgccatag ccatttccag ctgaaaatcg gcatcagaca
     3061 gctgggaatc tagttcgcca acgcgctttc gcctctcttc agccattctg agaagaccga
     3121 aggtctcctc atggccctcc atcgcacgct ccgcacgggc aagcgtgtca tggaccgcct
     3181 gtcgggatac cccataaagc tcggatatct ccgcaagcga gtagtcgttc aagtagtaga
     3241 gcacgcacat ttcccgctgg tgttcggtaa gcagaggacc atgaacatca agaagagcgc
     3301 ccatcctgag catcttgtcg agcatcggcc ccgaccacct tgcaagtgtt aaggtctata
     3361 cctttacacc acgcagtata cagggatgag cggcccttgt caatatggac ttcgcgtatg
     3421 gacctttgaa gctcgccaga gcatcaagga gcatgcccac tgccgcagca caggattact
     3481 cagcctgcag acattccgcc ttcaagcgtt tcaaaccact tgtgcctata aggaccaaaa
     3541 tcctgtgtac ggttacgcag acggacgcct gcacaattcg gaaggaaagc ggccccgcag
     3601 ggccgcaact tctaaacaag tctcaacacg tttcctggca atccccggat ctgcattgcc
     3661 cgcaattcgg agcctagctg ctcttttaga ctaaagtatt cttgctagtt actcagcgtt
     3721 tagcaccttt ccggccatca tcacctgcgt ccgctctaag aagcccctga acaaaccgtt
     3781 cgggactgaa aggtctgaga tcttccgccc cttcacctac tcctacatat ttcaccggga
     3841 tgttcaattc ctggctgatg gcaaccacga tgccccctct ggctgttccg tccaacttcg
     3901 tcaagaccac gccagtgact cccaccgcct cattgaaaac ccgcgcctgc gctatggcat
     3961 tctgtcctgt cgtagcgtca actacgagca agacttcatg tggagcttca tcgcactccc
     4021 gcgagatcac cctcttgatc ttcttcaact cttccatgag gttgctcttg gtgtgaagcc
     4081 tgccagcggt gtcaacaagc aggtagtcgg agccacgggc ccgagcagcc tggtaggcat
     4141 cgaaagccac agccgccgga tcggatccct cctgatgtcg tactacattg cacccagtcc
     4201 tgttacccca aacctccaac tgttctatgg cagctgcacg aaaagtatca gctgcagcaa
     4261 gcgtcactct gttcccctca tgagccaaca tggatgctat cttccctatc gatgtagtct
     4321 ttcccgtccc attgaccccg accaccatca ctacggtcgg tggctcgtca gcccttgcaa
     4381 gaggcgcggt cctttcgcca agtatcgaga tgagctcctg ctctatcaag gccacggccc
     4441 cgtcggcatc gacctgctct ccacgcgaac cggattcccg cattttgttg attatcgcag
     4501 tggtcgctgc aacgcccagg tctgcctgta tgagaactgc ctccaattcc tcaagggtct
     4561 cctcgtctat gaccttcttc ttggagaaca aggcctcgat cggatcgata atggcatccc
     4621 tcgtctttgc gagtccactc caaaggtttg agaaaaatga cggtgacatt gacatacctc
     4681 catgccccta gctgacctgc tgatccgttc gaatcgagaa gaccttcgac actcccgact
     4741 cttccattgt cacgccaaac agcgcgtcag cacactccat ggtccgtttt ctgtgggtca
     4801 caataattac ctgcacgtcc tcggatgtcg tcttgagcaa ctgggcaaac cgtattaggt
     4861 tagcctcgtc gagcgccgaa tcgatctcgt caaggagaca gactgggctc ggattcactc
     4921 tcatgatcgc aaacaacagg gcaatggcaa ccagcgacct ttcgcccctt gacattagag
     4981 aaaggctgga gagtttcttg cccggaggct gacatactat atcgatccca cactcaagag
     5041 gattgtcagg atcatcaaga acaaggtcgg cggttccacc gccgaatatg tcggtgaata
     5101 tgtcggcaaa ctccgatctg accgcctcga acgtctcaag gaatctcttc tcacatgtaa
     5161 gctcgcattc ccgaactacc tcatcgagtg aagcctgcgc agcctccagg tccttcaatt
     5221 gagtttccaa gaagccgtgc ctctcagcaa gcttgcggct ttcctcaatg gcggtgtggt
     5281 taaccggacc gatttcagtg atctcggcac gcagttgcgc gatctctgcc tgaaccccgg
     5341 ccctagactg aattgcaaca tctctggcaa gtccttcctc ttcagatatg gaataggcag
     5401 aagccagccg ttcaacacat gcctcgtatt ccgctgcaat ccgcgcccct tctattctag
     5461 ccccgttcag tttctcagcc acctcttggt gccgtttccg ggccacacgc gctgccaact
     5521 cgccctcatt gacatcagca gctgccttcg cccgctcctc acggccctcg tcgagcagac
//
LOCUS       DAOSAI010000133         5567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9202_length_5567_cov_3.650399, whole genome shotgun sequence.
ACCESSION   DAOSAI010000133 DAOSAI010000000
VERSION     DAOSAI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9202_length_5567_cov_3.650399"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1210
                     /locus_tag="PLM83_05700"
     CDS             269..1210
                     /locus_tag="PLM83_05700"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOK70934.1"
                     /translation="MNFDERKDLLGLKDLSKEEIELILDTAVPMKDVIRREIKKVPAL
                     RGKGMITVFYENSTRTRTSFELAGKYLSADTSNLAVSTSSVQKGESLKDTIKTIEMMG
                     PDILVMRHSMSGAPHYVARNTTMRVINAGDGTNEHPTQALLDMFSIRERKGTLEGLKV
                     AIVGDILHSRVARSNIYGLSKFGCEVRVVGPSTLMPPEIERLGVKQYFDLKEGISGVD
                     VINVLRIQRERQRTGLFPSLDEYAELYMLRPEHLKFAGDDAIVLHPGPMNRGIEISTE
                     LAESPNTLVNEQVANGVAVRMALLFLMMGGRKDEIAA"
     gene            1194..2504
                     /locus_tag="PLM83_05705"
     CDS             1194..2504
                     /locus_tag="PLM83_05705"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOK70935.1"
                     /translation="MRLLLRGGRVVDPANRINTVTCVLVEDGRIAAIGDIPGEESADS
                     GANVIDCSGKIVCPGFIDLHAHLREPGYEYKEDILTGSRAAAAGGFTTVCAMPNTNPV
                     VDNGAVAGYVARRAREVGIVNVLPIGSITKGQDGEELSEMAELVQAGCVAVSDDGKPV
                     MRSDIMRHALDYARMFDIPVLSHCEDINLSRDGQMHEGFRSTIYGLQGIPAEAEEIMV
                     SRDVMLAKLTGGRLHICHVSTIGSLKIIQRAKAEGVRITCEATPHHLTLTDEAVGSYD
                     ADTKVNPPLRSEEHVAALREAVASGLVDCIATDHAPHHVESKDCEYGAAAFGISGLET
                     AVALIMDRLVNTGALQLCTAITLLTTRPAKILGIDKGTLTPGSPADITVIDPDEVRTV
                     DPRRFYSKGKNTPYKGVQLRGWPWMTIVDGRIVFKEGIVTEMKL"
     gene            2504..3388
                     /locus_tag="PLM83_05710"
     CDS             2504..3388
                     /locus_tag="PLM83_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOK70936.1"
                     /translation="MERVEEGTVCVETATIIRNDHVGVGLWEMEVAAPRTVAARPRPG
                     QFIHACTTPPYLAGEHAHPLLRRPFSLYDVDTERESISILYKARGTGSSLMTRFRTGD
                     LVDIMGPLGRGFSVPRRTHTPCALLVGGGVGIAPLVYLARVLVHSGWKVDVLYGANTC
                     GELVALYRLQQLGASCHCTTLDESSGARGVVTDLLPRALEECKPDAVYTCGPEQMMAT
                     VARFAQEFNISGEASLEAHMACGIGACLGCARKLRGSDEGYVKVCVDGPVFPLDSIEF
                     EAEADFGGEEVAGCERKD"
     gene            3370..4299
                     /locus_tag="PLM83_05715"
     CDS             3370..4299
                     /locus_tag="PLM83_05715"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOK70937.1"
                     /translation="MRAEGLAPNLGVDLGGIKLANPVVMASGTFGYGMDYIDLVDPAS
                     VGAVVVKGTTLRPRAGNPPPRIVETPCGMLNAIGLENPGIRVFLDEHLPSLLDRHVTV
                     IANIAGETVDEYAELAAIIDGHGGVAGIEINISCPNVARGGMHFGADPGAAREVVSAV
                     RSVTSLPVIPKLSPNVADIVAIASACQDAGADAISMINTLAGMAIDVENRCPVLGNVF
                     GGLSGPAIKPVALRMIYQVAREVDIPILGGGGIISAKDALEFHMAGATAVSIGTGTFI
                     RPTLAMEVIEGVRDYLISHDLESIDQIIGAALK"
     gene            4335..5102
                     /gene="pyrF"
                     /locus_tag="PLM83_05720"
     CDS             4335..5102
                     /gene="pyrF"
                     /locus_tag="PLM83_05720"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOK70938.1"
                     /translation="MEPKDALIVALDVDTKKEALAIAHELAGEAGAFKIGMQLFNSEG
                     PDVVRSIQKLGGQVFVDLKFHDIPNTVAAAGRIMARLGCAMFTVHAAGGTHMLKALAE
                     AVKDESAACGADEPLILAVTVLTSIGQEQLDKEMLIHDASVEEVAASWAKLAQECGAG
                     GVVCSPREIVAVRKACGPEIKIVTPGIRPSWSAGLAADDQRRITTPAKAIALGADYIV
                     VGRPITRAENRLEAARRIVMEIAEAKAEKERCLAYGQ"
     gene            5092..>5567
                     /gene="pyrE"
                     /locus_tag="PLM83_05725"
     CDS             5092..>5567
                     /gene="pyrE"
                     /locus_tag="PLM83_05725"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOK70939.1"
                     /translation="MGNEPGCNDHLKEALEIFTESGALLDGHFLLTSGRHSDRYMQCA
                     QVLKFPQYTEKLARWLAEDFADDQIDLVIGPAMGGIIVAYETGRQLGVPAIFAERDSS
                     GTMALRRGFSITPCQRVLVVEDVTTTGGSVREVMSLVEKSGGIVAGVGVLVDRSNGA"
BASE COUNT         1335 a         1538 c         1642 g         1052 t
ORIGIN      
        1 gttgtccccg acgccctgcc gcgcgccctg tgggtcgatg aaatcgacgg cgagaacaaa
       61 gtcgtcatac aagaacttgc tgattgagtg tttccaatat aatagtccct ttaaactagt
      121 ccagtgaggc tggcaaggga aagtgcagca gcatgatgcc ggtgtgcctt ccctgcaacg
      181 cactgcgggg aaggtttctt gtaacctaaa gctgcggcaa cgattctgcc ttcgcaagga
      241 cagttacagg gcacaagggg gcgcgacggt gaactttgac gaaagaaaag atctattggg
      301 gctcaaggac ctcagcaagg aagagatcga actgatactc gatactgccg tccccatgaa
      361 ggacgtgatc aggcgcgaaa tcaaaaaggt gcccgctctt cgcggtaagg gcatgatcac
      421 tgtcttttat gagaacagca cccgtaccag aacctcattc gagcttgccg gaaagtatct
      481 cagcgctgac acatcaaatc ttgccgtatc aaccagcagc gttcagaaag gcgaaagcct
      541 caaggacacc ataaaaacca tcgagatgat gggccctgat atcctggtca tgcgccattc
      601 tatgagcgga gctccccatt acgtcgccag aaacaccact atgcgggtga taaacgctgg
      661 cgacggcaca aatgagcatc caacccaggc gctgcttgac atgttcagca tccgcgaacg
      721 caagggcacg ctggaagggc tgaaggtcgc gattgtcggc gacatcctcc acagccgcgt
      781 ggcacggtcg aacatatacg gcctgagcaa gttcggctgc gaagtgcgcg tagtaggccc
      841 gtccacattg atgcctcccg agatcgaacg gctcggggtc aagcagtatt tcgacctcaa
      901 agagggtatc agcggagtgg acgtcataaa tgtcctcaga attcaaaggg agcgacagcg
      961 tacaggactc ttcccttccc tcgacgaata tgccgaacta tacatgctcc gccccgaaca
     1021 tctcaagttc gcaggcgacg acgccatcgt cctccatccg gggccgatga acagaggcat
     1081 cgagatatct actgaacttg cggaaagtcc caacaccctt gtcaatgaac aagtcgcaaa
     1141 cggagttgca gtccgcatgg cactcctatt cctcatgatg ggaggtcgaa aagatgagat
     1201 tgctgcttag gggcggcagg gtcgtagacc cggctaaccg aattaacact gtaacttgtg
     1261 tcctggtgga agacggaagg atcgccgcca taggcgacat accaggcgag gaaagcgcgg
     1321 actctggcgc caacgtgata gactgctccg gaaagatcgt ctgccccgga ttcatcgatc
     1381 tccatgcaca tttgagggag ccaggatacg aatacaaaga ggatatcctg accgggtccc
     1441 gcgcagcggc agcaggagga ttcactacgg tatgtgccat gccgaataca aacccagtgg
     1501 tggacaacgg agcagtagca ggatacgtcg ccagaagggc gcgtgaagta ggcatcgtca
     1561 acgtactccc aatagggagc atcacaaaag gccaagacgg cgaggaactg agcgaaatgg
     1621 ctgagctggt ccaggcggga tgcgtagccg tttctgacga cggtaaaccg gtcatgaggt
     1681 ccgacatcat gcgccatgcc ctagactacg cccgcatgtt cgatattccc gtcctctccc
     1741 attgcgagga tatcaacctc tcccgcgacg gacagatgca tgaagggttc cgctccacca
     1801 tctacgggct ccaagggatt ccggccgagg ctgaggagat aatggtctca agagacgtga
     1861 tgctggcaaa gctcacagga ggtcggctcc acatctgcca cgtttcgact attgggagtc
     1921 tgaagatcat acagagagca aaggcagagg gagtccgcat cacctgcgag gcaacccccc
     1981 atcacctcac cctcaccgac gaggctgtgg gctcttacga tgccgacaca aaggtgaatc
     2041 cgcctctgcg ctccgaagaa cacgttgcgg ccttgcggga agctgtcgca tcaggtttgg
     2101 tggattgcat cgccaccgac cacgcgcccc atcatgtgga gagcaaggat tgcgagtacg
     2161 gcgctgcggc atttggaata tcaggccttg aaactgcagt ggccttaatt atggatagac
     2221 tagtcaacac gggagcattg cagctttgta ctgcgatcac tcttttaact acccgaccgg
     2281 ccaaaatcct cgggatcgac aaggggactc tgactccagg cagtcctgca gacatcactg
     2341 tcatagaccc cgacgaagtc aggaccgtgg atccgcgccg cttctactct aagggcaaaa
     2401 acactccata caagggagtg cagctcagag gatggccgtg gatgactatt gtggacggtc
     2461 gcatagtttt caaagaaggt atcgttacgg agatgaagct ttaatggaac gagtcgaaga
     2521 gggcaccgtt tgcgtggaaa cggcgacaat catcaggaac gatcatgtag gagtggggct
     2581 ttgggagatg gaagtcgccg cacctcggac cgtggcggca aggccgcgcc cggggcagtt
     2641 tatccacgcg tgcaccacgc ccccgtatct tgcgggcgaa catgcacacc cactcctccg
     2701 gaggcccttt agcctttacg atgtggacac ggaacgcgag agcatctcta ttctttacaa
     2761 ggctcgggga acggggtcat ccttgatgac gaggttcagg acaggcgacc tcgtcgacat
     2821 catgggccca ctaggaaggg ggttctccgt gccacgacgc acgcatacgc cgtgcgcgct
     2881 cctggtcggc gggggggtcg gaattgcgcc tctcgtgtat ctcgcccgcg tccttgtcca
     2941 ctcaggctgg aaagtcgatg tcttatacgg tgcaaacact tgcggggaac tagtagccct
     3001 ctaccggctg cagcaactgg gcgcctcatg ccattgcact acgctggatg aaagctcagg
     3061 agctcgtggg gtggtgacag atctcctccc ccgggcgctc gaagagtgca agcccgacgc
     3121 cgtatacacc tgtggccctg agcagatgat ggcgaccgtg gcccgatttg ctcaagagtt
     3181 caatatatcc ggagaagcat ccctcgaggc ccacatggcc tgtggcatcg gggcatgtct
     3241 cggctgcgcg cgaaagctcc ggggaagcga cgaaggctac gtgaaggtct gtgtggacgg
     3301 acccgtcttt cccttggaca gtatcgagtt tgaagccgag gcagacttcg gcggagagga
     3361 ggtcgcagga tgcgagcgga aggattagcc ccaaaccttg gagtcgacct cggagggatc
     3421 aagcttgcca accccgtggt aatggcgtca ggaaccttcg gatacggcat ggactacata
     3481 gatctggtgg atcccgccag cgtaggcgct gttgtggtca agggaacgac cttgcgcccg
     3541 cgcgccggaa acccgccacc gcggatagtc gagactccct gcgggatgct gaatgccatc
     3601 ggccttgaaa accccggcat aagggtcttt ctcgacgagc atctcccgtc tctccttgat
     3661 cgccatgtca cggtgatagc caacatagct ggggaaaccg tcgacgaata cgccgaactg
     3721 gctgcaatca ttgacggtca cggcggggta gccgggatag agataaacat ctcatgcccc
     3781 aacgtggcgc ggggaggaat gcactttgga gctgaccccg gcgcagcccg ggaagtagtg
     3841 agcgcggtcc gtagcgttac atcacttccg gtaatcccga aactctctcc caacgtcgcc
     3901 gatatcgtcg ccatagcctc ggcctgccag gatgcgggcg ctgacgccat atcgatgata
     3961 aacacgcttg cgggcatggc gattgacgtg gaaaacaggt gtcccgtatt ggggaacgtc
     4021 ttcggcgggc tttcaggacc cgccataaag ccagtagctc ttcgcatgat ataccaggtc
     4081 gcaagggagg tcgacattcc cattctgggc gggggcggta tcatctcggc caaggacgcc
     4141 ctggagtttc acatggcggg tgcaacggca gtatcgatcg ggacgggaac cttcatccgc
     4201 cccacacttg ccatggaagt cattgaagga gttagggatt atctgatctc ccacgatctc
     4261 gaaagcattg accagattat cggggcggct ctcaaatgag atggaaagac gccgccgcaa
     4321 aaggagcgtg cgatatggaa cctaaggacg ccctcatcgt agccctcgac gtcgacacca
     4381 aaaaagaagc tttggcaata gcgcacgagc tcgctggtga agccggcgcc tttaaaatcg
     4441 ggatgcagct tttcaacagc gaaggccctg atgtagtcag gagtatccaa aagctgggcg
     4501 gacaggtgtt cgtagacctc aagtttcacg acatccccaa cacggttgcg gctgcaggca
     4561 gaatcatggc gaggctcgga tgcgccatgt tcactgtcca tgcggcaggc ggaacccaca
     4621 tgctaaaagc cttggccgaa gctgtgaagg acgaaagtgc cgcttgcgga gcggatgagc
     4681 cgctcatcct cgctgtgacg gtgcttacca gcatcggtca ggaacaactc gataaagaga
     4741 tgttaataca cgatgcctcc gtcgaggaag tggctgcatc ctgggccaaa ctggcgcagg
     4801 aatgcggcgc aggcggcgtg gtctgttcac cccgggagat agtcgccgtg cggaaagcgt
     4861 gcggacccga aatcaagatc gtcacccccg gaattcgtcc atcttggagc gcaggtctgg
     4921 ccgcagacga ccaacgcaga ataactactc cagcgaaagc catcgccctc ggcgcggatt
     4981 acatagttgt gggacgcccc atcacccgag ccgaaaacag acttgaagct gcaaggagaa
     5041 tagtgatgga gattgccgaa gctaaggccg aaaaagagag gtgtttggca tatgggcaat
     5101 gaacctggct gcaacgacca tctgaaggag gccctggaga tcttcaccga atccggagct
     5161 cttctcgatg gtcatttcct gctcacctcc ggaaggcaca gtgatagata catgcagtgc
     5221 gctcaggtcc tcaaattccc gcaatacact gaaaagttgg cacgctggct ggcagaggat
     5281 ttcgcggatg accagatcga cctcgttata ggcccggcta tgggcggcat catcgtcgcc
     5341 tacgaaaccg gacgacaact gggtgtgccc gcaatcttcg cagagcgcga cagttcgggc
     5401 acgatggccc tccgtcgggg attttccatc accccatgcc aacgagtact cgtcgtagaa
     5461 gacgtcacaa ccaccggagg ctcagtgagg gaagtgatga gcctggtaga aaagtccggc
     5521 ggaatcgtag caggcgtggg ggttttggtg gacaggagca acggcgc
//
LOCUS       DAOSAI010000134         5536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9256_length_5536_cov_4.141762, whole genome shotgun sequence.
ACCESSION   DAOSAI010000134 DAOSAI010000000
VERSION     DAOSAI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9256_length_5536_cov_4.141762"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLM83_05730"
     CDS             complement(<1..925)
                     /locus_tag="PLM83_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70940.1"
                     /translation="MAWADGPFTAGAAVHSLLRYGHVYPLIRPGEAIPGFIRDRGFVF
                     PSWLVTDYLISLASKPFVIISGISGTGKTKMTQLVADYMSKVSGVDESTAFVPVRPDW
                     TDNSHLLGWYNAIAERYETTPVLDLIIKASDAPAAPHFIILDEMNIAKVEHYFSDFLG
                     CMESRWIDVDDRVRQEPIHLHSHVADYEAGEGSVSIPPWLDLPLNVYVVGTVNVDEST
                     YMFSPKVLDRANVIEFNDVCLDPDSQMRESTLADPKTPGSSGFVLRSDVNPVELLSGY
                     TPAPPKLWIRMKDEMPEHFELLLEVHEALRPY"
     gene            complement(1156..1734)
                     /locus_tag="PLM83_05735"
     CDS             complement(1156..1734)
                     /locus_tag="PLM83_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70941.1"
                     /translation="MPVSDVGVDKIVEALEYFDDTMRDTDAWQGWESRRSHLYAIRWR
                     DKLYPAWEAVRTATGSSGFRGGFQANQYLRARGFEVIPLRHDEIRFSQDILARLDRML
                     QKFAEYRSDEACYLAEERTYKEELFDHLGTIWDLLYTDPEASQKALIKFISGEDAPAG
                     LLAAFNNLQGGYGLPVRDDFLVYLRNTSADEY"
     gene            complement(1825..2451)
                     /locus_tag="PLM83_05740"
     CDS             complement(1825..2451)
                     /locus_tag="PLM83_05740"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOK70942.1"
                     /translation="MGAITVKGRGYVSAQPDVVTLSFDVGTKARDYAECVDRLTGRTA
                     ALRRDLAAAGADCAELKTTNFSVNVAYRYDKSRYYFDGYSASHRLEIELPADKDMLNR
                     VLGAVAKGHSGAEIRIRFSVKDTENLRQQALAEAVRVAKLSADSLAQAAGVKLGKIRS
                     IAHGWDEIRLSGPSYGVVCDSVSDAKVDIDPKDVSISEDVTVVYEIEE"
     gene            3007..3942
                     /locus_tag="PLM83_05745"
     CDS             3007..3942
                     /locus_tag="PLM83_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOK70943.1"
                     /translation="MTCDTDWSWPQIATLNLIMRKRVDWSLLRSGWTIPVDIHERLVD
                     ENDGVELQPKDKRHVVLLVDGHPFDAMLTCIRRPQGPPHILQIRYDTNQELRQVLEAT
                     FWTSRKYLQLERERLRRELGPNAKLYAEVPPHFAEFVEWRATGIPFYYHVDLVPCRFR
                     ASDIVKQTLTNLGEPHIEAILAEDSENRFAYWVTAIERITPEDSGEIARALRYCRRYL
                     GLAQRLLGQMYGHTCQLCGRQAQYSAGSMVNTTHHIDPVTTSMDNRISNLVLVCPTHH
                     AMLHAHNFTFDRTSLSFASPKMCVLKIAMDKHLGS"
     gene            complement(4260..4844)
                     /locus_tag="PLM83_05750"
     CDS             complement(4260..4844)
                     /locus_tag="PLM83_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70944.1"
                     /translation="MKVKHARVCVFVCLLVILLGAPGYAETESEMDILDVTQYRTLVD
                     EFGMPTTESVKEIAEAAQSTFDAGDYEAAIPLLEEWAQSANWLANMIVSGLKPFYRAS
                     YDDRKEFSYSKVAALVVYETTANSLKRDRNHAMVMIAECHVQTGRLDVAVSMYMKALN
                     LLDVNDWEWWTRAANGLFELIGVPPMMEKPSSRR"
BASE COUNT         1297 a         1532 c         1365 g         1342 t
ORIGIN      
        1 agtatgggcg aagcgcctca tggacctcaa gcagcagttc aaaatgctca ggcatctcgt
       61 ctttcattcg gatccacagc ttcggtggag caggcgtgta acctgaaagc agctcgacgg
      121 ggtttacatc cgacctaagc acgaatcccg atgagcccgg tgttttcgga tccgccagcg
      181 tactttcacg catctgtgaa tccgggtcaa ggcaaacatc gttgaactca attacgtttg
      241 ccctgtccag gactttgggc gagaacatgt acgtagactc atcgacgttc accgtgccaa
      301 cgacatacac gttaagcggc aagtcgagcc acgggggaat gctaactgag ccttcgccag
      361 cctcgtagtc ggccacgtgg ctgtgaaggt ggataggttc ctgtctcacc cgatcgtcta
      421 catctatcca tctgctctcc atgcagccta aaaagtcgga gaagtagtgc tccaccttgg
      481 ctatgttcat ctcgtcgagg atgatgaaat ggggcgctgc cggagcgtcg gatgctttga
      541 taatcaggtc aaggacggga gtagtctcat atcgctcggc aatggcgttg taccagccaa
      601 gaaggtgtga gttgtcggtc cagtcgggcc ttactggaac aaacgcggtg ctctcgtcca
      661 ctccagaaac cttggacatg taatcggcta ccagttgcgt cattttggtc ttgcctgtgc
      721 ccgatatccc cgagatgata acaaatggct tgctggcaag tgaaatcaga tagtcggtta
      781 cgagccacga ggggaataca aaccctcggt cgcggatgaa accgggtatt gcttctcctg
      841 ggcgtatgag aggatatacg tggccatatc gaagaagaga gtgtaccgct gctccggctg
      901 tgaagggacc gtcagcccaa gccactttgc agttgcctca taatcagtac tgcgatacag
      961 aatgtacgat tcgggatcat acgatgcgag caggatggcc gcgaaattca gcggcatcga
     1021 tggccgagac tttttgcctt cgtagaactt gccttcatca tataagcggt cataggcggc
     1081 tttggtcgcc tgtcggaact ggctgaggcg gaccgccggg gtggcctcac cctcaaatag
     1141 ctgcaacatc atggttcagt attcatcagc gcttgtgttt ctcaggtata ccaggaagtc
     1201 gtcgcggaca ggcaacccgt aaccaccctg gaggttgttg aaggctgcta aaagcccggc
     1261 tggggcatcc tcacccgaga tgaacttgat aagcgccttt tggctggctt ccgggtcggt
     1321 gtacagcaga tcccagatgg tgccgagatg atcgaagagc tcctccttgt aggtgcgttc
     1381 ctctgcaagg tagcaggctt cgtcggacct gtactcggcg aacttctgaa gcatcctgtc
     1441 caatcttgcg aggatgtctt gcgaaaaacg gatttcgtca tggcgcaaag gaatgacctc
     1501 gaagccacga gcccgaagat actgattggc ttggaatccc ccgcgaaacc cggaagaccc
     1561 ggtagcagtg cgaaccgcct cccaggcagg atacaacttg tcgcgccatc gtatcgcgta
     1621 caagtggctt cggcgcgact cccatccctg ccaggcatcg gtgtcacgca tcgtgtcatc
     1681 gaaatactcc agtgcctcta caatcttgtc cacgcccaca tcggaaacag gcatgatcac
     1741 accacctcca aggttccagt cggcccgatt gccttatccc gttcatgctg ctcgaccgtc
     1801 ccccacatga acgggcattg tgatttactc ttcgatctca tagacgaccg tcacgtcttc
     1861 gctgatggat acatctttcg ggtctatgtc caccttagca tctgaaacgc tatcgcatac
     1921 aacaccgtag gaagggccgg acagacgaat ctcatcccaa ccatgtgcta tgctccggat
     1981 tttacccagt tttacacctg ccgcttgagc taacgagtcg gcgctgagct tggcaaccct
     2041 gacagcctcc gcgagagcct gttgcctcag attctctgtg tctttgactg agaagcggat
     2101 ccgtatctct gcgccgctgt ggcccttggc aactgcgccc aacacacggt tgagcatgtc
     2161 cttgtccgcg ggtagttcaa tttccagacg atgcgaagcc gagtacccat cgaagtagta
     2221 acggctctta tcatagcgat atgcaacgtt cacactgaag ttagtagtct tcagttctgc
     2281 acagtcagcg ccagcagctg ctaagtcccg gcgtagggcc gccgtacgcc cggtgagcct
     2341 atctacgcac tccgcgtaat ctcgcgcctt ggttccgaca tcaaaagaca acgtcacgac
     2401 atcgggctgg gccgacacgt agcccctacc tttaaccgta atagcgccca tgagacaccc
     2461 tccctggcat ataatatgga gcctcgcaaa tggcatcgat gtgctgccac agtcgacaca
     2521 ttgagtgtta tccataccga cgaaagagac ctcttatggg gttatcgcgg cttctttcag
     2581 ggtaggcagc cagcagtatt tgactttgtc atagagtggt tactgttggc atgtatgttt
     2641 atgaatccct ggaacgaagt gcgctttcct tccgtcattt cgggcttgat acattcgtat
     2701 tctaaaaagc aatcaattcg catcttggcc tgcatcattt caccagccca gccctgagat
     2761 tgactgcaca tgctcagttc cacaccttgc tccacgcaca ttaggacttc gatgttgtag
     2821 cctcggttcc cctgctcggt ctttttcaag cggtcacaga cggactacgc tttccgttcc
     2881 tctaagcttg gcagacactt cgcctttgca atgactagct agtggcctgc tccaattccc
     2941 ccacaactct aaaggtatac tgtccgattt tagcgaatct tcatactgat tggaggcgtt
     3001 cgacaggtga catgcgatac tgactggagc tggccccaga ttgctacttt gaacttgatc
     3061 atgaggaagc gggttgactg gtctttactc agaagtggat ggactatccc agtagacatc
     3121 catgagcggt tggtggatga aaatgatggg gtagaactgc agccgaaaga taaacgccat
     3181 gttgtgctgc tcgtcgatgg tcatcctttc gacgcaatgc taacttgtat tcgacgtccg
     3241 caaggcccgc cacacatcct gcagatcaga tatgacacga accaagagct acggcaggtc
     3301 ctcgaggcga cattctggac tagccggaag tatttgcagc tggagcgcga aaggctacga
     3361 cgagaacttg gcccaaacgc aaagctgtat gcagaggtgc caccgcactt cgcagagttt
     3421 gtagagtgga gggcgactgg tatccctttt tattaccatg tggatcttgt gccgtgcaga
     3481 ttccgagcct ccgatatagt caagcagact cttaccaatt tgggcgaacc ccatattgag
     3541 gcaatacttg cagaggactc agagaaccga ttcgcgtatt gggtgactgc gatagagcgc
     3601 attactcctg aagacagcgg agaaatcgcc agggctctgc gatactgccg acggtatctg
     3661 ggtttggctc agcgcctcct tggacagatg tacgggcaca cgtgccagct ttgcggcaga
     3721 caggcacaat actcggcggg cagcatggtg aatacaactc atcacatcga cccggtcaca
     3781 acctccatgg ataatcgaat ctcaaacttg gtcctggtgt gtccaacgca ccacgccatg
     3841 ctgcatgctc ataatttcac atttgatcgg actagtttga gcttcgcatc tccaaagatg
     3901 tgcgtattga aaattgctat ggacaagcat ttagggtcct gaagtacttt gcctgaaaca
     3961 tgtgatctca gctccacgct tgtagtttcc gtatatgctg gtcccaatga gccttgcacc
     4021 tgccccctaa cctgctgcat gttacttcca aggcgacatg gaggcaaagg cattgacaca
     4081 atcacgctgt cgcgaccgtg agacccctac aatagctccc gcaagcccat agagattgac
     4141 cattcgcctg agcacagttc aagtagccag ttatggcaaa ctgcccttta tctcgatatc
     4201 gaaggccgca acccaacact caacacgaac gctcgttgcg gccttatcgt ctcgcaatac
     4261 tagcgccttg acgaaggctt ctccatcatt ggaggcactc ctatcaactc aaaaaggcca
     4321 tttgcagccc tagtccacca ttcccagtcg tttacatcca gtaggttcag cgccttcata
     4381 tacatgctaa ctgctacatc aagccgtccc gtctgtacat ggcactcggc gatcatcacc
     4441 atggcatggt tgcgatccct tttgagacta tttgctgttg tctcatacac tacaagagca
     4501 gcaactttgg agtacgagaa ttccttgcgg tcatcatatg aagccctata gaaaggcttc
     4561 agacctgaca ctatcatgtt tgcgagccaa ttggcgcttt gtgcccactc ctctagtagc
     4621 ggtattgccg cctcataatc cccggcatcg aatgtggatt gtgcagcctc cgcaatctct
     4681 ttgaccgatt ctgttgtagg cattccaaac tcgtcaacga gagttcggta ctgagtcacg
     4741 tcaagaatat ccatttcgga ttcagtctct gcgtatcctg gcgcacccaa caaaataacg
     4801 agaaggcaaa cgaaaacaca aacccgagcg tgcttcactt tcactagaaa tgcactccct
     4861 tcttgtctag gatccagttc ctattatcca tcccggacgg tgccgacgat gtacagcaat
     4921 agtagatgct aggtcgactt gtacgcccaa gtcaggaata tggcaaatag tctgtcgtgc
     4981 ctttgagcag gacaaggccc agtcattcat ggtactatgt acacattcaa tgaataatct
     5041 tcgtcctgcg atgatatggt ctgcctcaac gagttcatga gtcgtggggg caatcgttga
     5101 tggtcatttg agtgaaatga gacaatcccg catgccctgc tgcaaaacga gcctcattgg
     5161 ctgtctgtca tatcagtatc ctgggtaact aaagcctcta ccataaggat tatccttctt
     5221 gcccaattgg ccttcccgtt attctgcttg accggattct ggtggagctt caatgctgag
     5281 aaggcggctt gcaacgctgc tttagcgcaa cggccagccg tgacccaaac agcagcaaca
     5341 tggtggtgac tatcgggccc tttcaggaaa cgcttccagc gcatgctagt acaaccgggc
     5401 ggtccacggt accctcccaa ccgatgtgca ccatcaatat tgtcgagttc tatggaggga
     5461 ctaacactac tcctttccac cccatactac cagtacctgc acaacatctc cgtatttctc
     5521 atactctcgg agtcta
//
LOCUS       DAOSAI010000135         5529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9274_length_5529_cov_4.080015, whole genome shotgun sequence.
ACCESSION   DAOSAI010000135 DAOSAI010000000
VERSION     DAOSAI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9274_length_5529_cov_4.080015"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLM83_05755"
     CDS             complement(<1..873)
                     /locus_tag="PLM83_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70945.1"
                     /translation="MSEEKTAARRQRGHGPRGAMMPGEKARDFKGSLKRLFGYLKPHK
                     TQLIIVMVLSIACVVLTIAGPKILGKATNILFEGVIGKQLPPGLGKEQIISMLKAAGK
                     DQMAEMVAGMNIVPGAGVDFEAIKRILLILVGIYIANALFSWLQHYIMAGISQRTVYS
                     LRKEADAKLGRLPLKYYDSNTRGEILSRVTNDIDNIASTLQQSLTQLVTATFTVIGVL
                     IMMFSISPLLAAIALVVLPLGVIVTLFIAKRSQKQFAEQWDSTGALNGHIEEMYTGHR
                     IVKAFNRQQEAIDEF"
     gene            complement(870..2609)
                     /locus_tag="PLM83_05760"
     CDS             complement(870..2609)
                     /locus_tag="PLM83_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70946.1"
                     /translation="MTKLLQYLKPYQKQLFLVMALLLVQAIANLYLPDLNADIINNGI
                     ARGDTEYILKTGGFMLLVTLLLAACSVVSVYWGSKTAMSFGRDLRSDVFRKVQSFSQA
                     EVDQFGTPSLITRTTNDVQQVQMAVLITLNVMISAPVMCIGGIIMALRQDVPLSISII
                     IIVPVMAVVIGVIMRRALPLFKSMQVKIDRLNQVVREKLAGVRVIRAFVKTDYEEARF
                     DEANLDLTTTALKINRMVAITMPLLMLIVNISMAAIMWFGAHRIDSGAMPIGNLTAFI
                     AYTMQILIAVMMATVMFIMIPRASASAERILEVLETEPSISAPKAPVALNPGTADSDA
                     SVEFRDVEFTYPGAETPVLRGISFIAYPGETTAIVGSTGCGKSTLINLIPRFYDVTGG
                     SLLIDGVDIREIPEEDLRNKIGFVPQKAFLFSGTIASNLRYGKRDATDDELWHALRIA
                     QAADFVAEMPGQLEAPVSQGGTNFSGGQKQRLAIARALVKRPKIYVFDDSFSALDFKT
                     DAALRAALNDEVDDAIVFIVAQRVSTIMNAERIIVLDKGEIVGMGTHEELMKLCSVYR
                     EIVLSQLSEEEVA"
     gene            complement(2640..3119)
                     /locus_tag="PLM83_05765"
     CDS             complement(2640..3119)
                     /locus_tag="PLM83_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOK70947.1"
                     /translation="MHSSTYDPTPVTELYGVDPLSLRVFHSFFKAMRLYRQLIFKVLS
                     EKEIYPGQAGCLWVLSGHDGIAQRDLARKLHVASSTVTVMLQKMEAAGTIVRKTDEED
                     QRLSRIYLTDAGRKIQEEMNEALTDFVNATFGKMPSGDREEFDRLLSALCSNISQEL"
     gene            3751..4305
                     /locus_tag="PLM83_05770"
     CDS             3751..4305
                     /locus_tag="PLM83_05770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HOK70948.1"
                     /translation="MNGELEKMLSGELYDAGDPELYRMRLRARRLTREFNATTEEEHD
                     LRVTILKELFASVGSAFIEPPFYCDYGSNICVGKNFYMNFNCVILDCAAVEIGDNVMA
                     GPGVHIYTAYHPLDAAVRVQGLELAAPVKIGNNVWIGGGAIICPGVVIGDNTTIGAGS
                     VVTRDVRANVFAAGNPCRVMRDLE"
     gene            4434..5162
                     /locus_tag="PLM83_05775"
     CDS             4434..5162
                     /locus_tag="PLM83_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70949.1"
                     /translation="MDLLVDADILACQYAYVYEASRGWDAGDDFDIDDAGWAISGFDS
                     AVVRMMNETGTENAILCFTGRDNFRYSILPTYKSNRRGKPRPVLLDALIEHAWDIWNC
                     KSVDCLEADDVMGIMATRWPGRYVVASTDKDLKQIPGLHYNWKKERVAEVDQRRADYW
                     FYFQVLTGDSTDGYAGCPGIGECRARRVLEDAAPDMWARVVAAFESRGLTEEDALIQA
                     RVARILRADDYDFKESQPILWSPA"
BASE COUNT         1136 a         1386 c         1625 g         1382 t
ORIGIN      
        1 gaactcgtcg atcgcctctt gctggcggtt gaaggccttt actatccgat ggccggtgta
       61 catttcctct atatgtccgt tgagcgcgcc ggtgctgtcc cattgctccg caaactgctt
      121 ctgggagcgt ttggcgatga acagcgtgac gataacgccc aagggcaata ctaccagagc
      181 aattgcggca agcagcgggc tgatggaaaa catcattatc aacacgccga ttaccgtaaa
      241 cgtcgccgta accagctgcg tcaggctttg ctgaagcgtg ctggcgatgt tgtctatgtc
      301 atttgtcacc cggctcagga tctctccccg cgtgttgctg tcgtagtatt tcagaggcag
      361 cctcccaagt tttgcatccg cctctttgcg caggctgtac acggtgcgct gggagattcc
      421 ggccatgatg tagtgctgca gccaactgaa caaggcgttg gcgatgtata ttccgacaag
      481 tatgagcagt attcgcttga tggcctcgaa atcgacgccg gcgccgggga cgatgttcat
      541 tcccgccacc atctcggcca tctggtcctt gccggcagcc ttgagcatgc taatgatctg
      601 ttccttgcca agtccaggag gaagctgttt cccgatgact ccttcaaaga ggatattcgt
      661 ggctttgccc aggatcttcg gcccagcaat ggtcaggacg acgcaggcta tggataaaac
      721 catgacgatg atgagctgcg tcttgtgcgg tttgagatac ccgaacagcc tcttcaacga
      781 ccccttgaag tcgcgcgctt tttctccggg catcatggcg cctcggggac catgtcctcg
      841 ctggcgtcgt gctgctgttt tctcttcact catgcgactt cctcctctga aagctgtgag
      901 aggacgatct cacgatatac ggaacagagc ttcatcagtt cctcatgtgt ccccattccc
      961 acgatctcgc ctttgtcgag cacgattatg cgttcggcat tcatgatagt gctcacacgc
     1021 tgcgccacga tgaagactat ggcgtcatcc acttcgtcat tgagcgcggc gcgaagtgcg
     1081 gcgtcggtct tgaagtcaag tgcggagaag ctgtcatcga agacgtatat cttcggcctt
     1141 ttcaccaagg ctcgcgcgat ggctaggcgc tgcttctgcc ctcccgagaa gttcgttcct
     1201 ccctgggaga ctggggcttc aagctgtccg ggcatctcgg cgacgaagtc ggctgcctgg
     1261 gctatgcgca gggcatgcca gagttcgtcg tcggtggcat cgcgctttcc gtagcgcagg
     1321 ttgctggcaa ttgtcccgct aaaaaggaag gctttctgtg ggacaaagcc tatcttgttc
     1381 ctcaggtcct cctcaggtat ttcccgtatg tctactccat ctatgagcag gctgccgccg
     1441 gtcacatcgt agaagcgggg gatgaggttg atcagagtgg acttcccgca tcctgtgctg
     1501 ccgacgatgg cggtggtttc gcccggatag gcgatgaagg agattccgcg caggaccgga
     1561 gtctctgcgc ccgggtatgt gaactctaca tccctaaatt cgacgctcgc gtcggagtcg
     1621 gcggtgcctg ggttgagcgc gaccggcgcc ttgggggcgg agatagacgg ctcagtctcg
     1681 agaacttcga ggattcgttc tgccgaggca ctggcccgcg ggatcatgat gaacatcact
     1741 gtggccatca tcaccgcaat gaggatttgc atggtgtagg cgatgaaggc tgtaaggttt
     1801 cctatgggca tggccccgct gtcgattctg tgtgcgccga accacataat ggctgccatg
     1861 gagatgttga ctatgagcat cagcagcggc atggtgatgg cgaccatcct gttgatcttc
     1921 aaggctgtgg ttgtgagatc gaggttggcc tcatcgaatc gcgcctcttc gtagtcggtt
     1981 ttaacaaagg ctctgatgac ccggactccg gcaagcttct cgcggactac ctggttgagc
     2041 ctgtctattt ttacctgcat cgacttgaat agcggcaggg cgcgtcgcat gatcacaccg
     2101 atgaccacgg ccattaccgg cacaatgatg atgatggata tggacaatgg cacgtcctgg
     2161 cggagagcca tgatgatgcc gccgatgcac attacagggg cggagatcat gacgtttagg
     2221 gttatgagga ctgccatctg tacctgctgc acgtcgttgg tggtgcgggt tatgagtgag
     2281 ggtgtaccga attggtccac ctctgcctgg gaaaaactct gcaccttgcg gaaaacgtcg
     2341 ctcctgaggt ctcggccgaa ggacattgct gttttcgatc cccaatatac cgagaccacg
     2401 gagcatgccg ccagcagtaa ggtgacgagc agcatgaacc cgcctgtctt caggatgtat
     2461 tcggtgtcgc ccctagcgat gccgttgttg atgatatcgg cattgaggtc gggcaggtac
     2521 aaattggcaa tagcctgtac cagaaggagt gccattacga ggaacagctg cttttggtag
     2581 ggcttgaggt attgaagtag ttttgtcatt cagaaactga cacctctcat gtaagcaaat
     2641 caaagctcct gtgatatgtt ggaacacagg gcgctcagga gcctgtcgaa ttcttcgcgg
     2701 tcgccggaag gcattttgcc gaaggtggcg ttgacgaagt ccgtaagcgc ctcgttcatc
     2761 tcttcctgta ttttgcgtcc tgcatcagtc aagtatatgc gggacagtcg ttggtcctcc
     2821 tcatccgttt ttcggactat cgtgcctgca gcctccatct tctgaagcat cacggttacc
     2881 gtggatgacg ccacatgaag ctttcgggct aggtcccgct gggcaatgcc gtcatgaccg
     2941 ctgaggaccc agaggcatcc ggcttgtccg gggtaaattt ccttttcaga cagcactttg
     3001 aagattagct ggcgatacaa gcgcattgcc ttgaaaaagg aatggaagac gcgcaacgat
     3061 agaggatcga ctccataaag ctctgtgacg ggcgtgggat catatgtcga tgaatgcact
     3121 gttagacccc ctttcagcga tcgtaggcca ggccccgagc acataatcgt tgccatgcta
     3181 ataggtagca tgctaactat atacgtaatg tattgtagcg cgtcttggct gcgaggtcaa
     3241 cagggatgtg ttgaatgtgg ctgggaatca gtagtatgaa gctcttagcg tctgggcggg
     3301 ctccgtggga ggggatttcg cgtccccgta cacgctgcaa actctatccg ctgctaatag
     3361 aggggtttag gtccgtttcg ccaacgcctg ctgcgctcaa ttcgaatgct ctccctcctg
     3421 accttttccg gctatactgc gaatcgtcag atttgccctg catagcatag gtatgtccag
     3481 tccgaatgct tctatgagct tggatagata ctctactgcc tgtagtatgc ctttttgttt
     3541 cccacagcgg agctattcga ttgtccgtat aagactttga aagctcaaag cttcggtgtt
     3601 cgcaccaacg tgttggcgtg gccgagttga gattcttcca ttacaatgac agttgacaag
     3661 cggcatcttt aggggcggga gccatgctcc gatgttctgc cttttagtct caggtggcat
     3721 attgtccact ttgccaaagg gggctccgat atgaacgggg aattggaaaa aatgctctcg
     3781 ggcgagcttt acgacgccgg tgaccctgag ttgtaccgta tgcgtctcag agctcgccgg
     3841 ttgacccggg agtttaacgc cacaacggaa gaggaacacg acttgcgtgt gacgattttg
     3901 aaggagctgt ttgcatcggt gggcagcgcc ttcattgagc cgcctttcta ctgcgattat
     3961 ggaagtaaca tatgcgtcgg caagaacttc tacatgaatt tcaactgcgt cattcttgac
     4021 tgcgctgcag ttgagatcgg tgacaacgtc atggccggtc ccggggttca tatctacact
     4081 gcataccacc cgcttgatgc cgcagtgagg gtgcaaggcc ttgaacttgc ggcgccagtc
     4141 aaaataggaa acaacgtctg gataggcgga ggggccataa tatgccctgg ggttgtcatc
     4201 ggcgataaca caacgatcgg agcgggaagc gtggtgacta gggatgttcg agccaatgtg
     4261 ttcgcagcgg ggaatccctg tcgggtgatg agagacttgg agtagcgctc atttttcccg
     4321 ctcgggaacg gcggcggaat cgattgttga tggaggagaa aggagtgggc tgcccgaacg
     4381 cttattagaa ctgatgcagc ctttcgattg tatatatttg atgggggacg tgtatggatc
     4441 ttcttgttga tgccgacatt ctggcgtgcc agtacgcgta tgtgtatgaa gcgagccgtg
     4501 gatgggacgc gggcgatgac tttgatatag atgacgcagg ctgggcgatt tctgggtttg
     4561 attcggcggt tgttcggatg atgaacgaga cgggaacgga aaatgcgatc ctttgcttta
     4621 ccggcaggga taacttcagg tactcgatac tgcctacata caaatcgaac cggcggggca
     4681 agcccaggcc ggtgcttctg gatgcgctta ttgaacatgc gtgggatata tggaactgca
     4741 agtcagtcga ttgccttgag gcggatgatg tgatggggat tatggcgact aggtggcctg
     4801 ggcgctatgt agtggccagc accgacaagg atttgaagca gattcccggg ctccattaca
     4861 actggaagaa ggagagggtt gcagaagtcg atcagcgccg ggctgattac tggttctatt
     4921 ttcaggtgct caccggtgat tctacagatg ggtatgcggg gtgtcccggc attggcgaat
     4981 gcagggccag gcgggttttg gaggatgctg cccctgacat gtgggcgcgg gttgtggccg
     5041 ccttcgagtc ccgggggttg actgaggagg acgctcttat tcaggcaagg gtagcccgaa
     5101 tcctgcgagc agatgactac gatttcaaag agagccagcc tatcctctgg agcccggctt
     5161 gaatgtgtaa taagtctgct cccgtcggtg gcttcggtgc cgatcttagc agtgataaac
     5221 cacattgtct gtaccgattt tctgccttcc ccgcggcttt gctgcagata tgcatgtatc
     5281 gcgggttttg gttgtgatga cgccttccgg cgcttgtctc ttgcaaggcg ttgacgggga
     5341 tggttctgat tctccttcag atgcctgagg atgtgcggcc caacttattt ctagctttga
     5401 ttgggaaata ccggatttgg ctcacagtca accaatggag cgctcctcgc ggccacctgg
     5461 gattgaaaac ccaacctgtc aaccgactgt gaaaatgtca ccccttttgg gggttaatcc
     5521 gtctgagaa
//
LOCUS       DAOSAI010000136         5523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9289_length_5523_cov_4.959400, whole genome shotgun sequence.
ACCESSION   DAOSAI010000136 DAOSAI010000000
VERSION     DAOSAI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9289_length_5523_cov_4.959400"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1159)
                     /locus_tag="PLM83_05780"
     CDS             complement(254..1159)
                     /locus_tag="PLM83_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70950.1"
                     /translation="MISRRGIVIIVLIGLAVVASSTHPWVSGSNLLADKYSSMFDPYI
                     NTDTGIIAKAQFEEAFSAAGIKCVAIQVPVGDITVIKTGGNNITIDGERIAYGETLEK
                     AETKLSLVPLEARTRDDVLEIISLVDTSATTGSSSRINMVIQVPAGIDVRSTLSAGEI
                     TLNGIDGAIYAKVDTGKVNTDSTRGDLDIEVSVGAIDVRDAVVANKLSLITSMGPIDF
                     LGTLGKNNYIESSAGPIKISVPRSTCVRIDAAISAGQINSSLPITGLNSDNQKSSATG
                     ILGNGTPTGELKVRASMGEISISEL"
     gene            complement(1199..1981)
                     /locus_tag="PLM83_05785"
     CDS             complement(1199..1981)
                     /locus_tag="PLM83_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70951.1"
                     /translation="MKRWWSLVGKEWRGSWTVILLALAATIGFDTWLAIKAPVWSARI
                     NGGLAFGLSFIPFVGMGIAALVVGYYTMRNEWRDRTSLQLLSYPVSGAGILWAKLLVA
                     VLGLGLITVVATVGTWFVTQRSSIPYSTIMSATEDILRLTPWERTLDIIWMVVCSILG
                     TAFIVAVGLFSFTIGTIVPRISGLIAVIVYGLTWYLTFAFAPHAMYAFSFVPNPGLIV
                     YTGLGPMAARKVIPLLPFWPTIMGTVILILIAGKILELEVDA"
     gene            complement(1983..2675)
                     /locus_tag="PLM83_05790"
     CDS             complement(1983..2675)
                     /locus_tag="PLM83_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70952.1"
                     /translation="MPAIELTDVHKRYYRSHALRGVNLSVDRGKIVGLLGPNGSGKST
                     LLRICAGLASPTSGKVQVHGQDISIATKAITAYIPDIDSFYSWMTVRQAIAFESSMFD
                     DFVLADAMDLVRELKLDPDNKISSLSKGQRGRLKLVLAMSRRAELVLMDEPLSGIDPP
                     SRTAILETIADRYRAGEQTIIISTHEVLESEKLFEDVIFLSEGRIVLTGEADQLRAER
                     GKSLNEIFAEVC"
     gene            complement(2675..3112)
                     /locus_tag="PLM83_05795"
     CDS             complement(2675..3112)
                     /locus_tag="PLM83_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK70953.1"
                     /translation="MGISFDESVPIYTQIMKSVLWDVATGALLPGDKLLSVRDFAKQA
                     KTNPNTVQRAYAELERMGVVETQRGQGTFVSASPEIAREVRSEMTTAAVESFVEEMRR
                     LRYSDSDIISLVSQQLAKTGTNSSNGAAQNGPSTTNDHDGGVQ"
     gene            3429..3505
                     /locus_tag="PLM83_05800"
     tRNA            3429..3505
                     /locus_tag="PLM83_05800"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3463..3465,aa:Met,seq:cat)
     gene            complement(3747..>5523)
                     /locus_tag="PLM83_05805"
     CDS             complement(3747..>5523)
                     /locus_tag="PLM83_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOK70954.1"
                     /translation="TVDAGPKRSAATGPNGTFTLSNVPAGRVILNASCPGYRPRQVNV
                     TVPAGNSINCEIELYPNTSGLATVRGCATVANNYYPEEDQSNHVLSAKTASRSLSAFQ
                     KAPLGQQPAPETEADTIQVKVRPGTDRDELAHNLLQAGYEIVDELPLTGIILIRPMQR
                     TARFLVESEAATLDSIDGVDWAYPDYPVYAAAVPNDPLYLYQWHYPAISLPEAWDIAT
                     GEGSPVIVAVIDTGIRPEHEDLQGKIVAGWDFIDKDSDPTDWPNSTKKYSHGTHVSGT
                     IAAATNNGVGVAGVSWGAQIMPLRVLGPDGKGSSSNVAEAIIWAVNSGADVINLSLGL
                     ANHPGSAMEDAVRYAFSRGVTMVAAAGNDSGAPVLYPAAFPEVIAVSATGQNNELASY
                     SSYGPEITVAAPGGTALFPVLSTGYSSANPAPNKYFLSQGTSMATPHVSGIAALLIAT
                     HGRMSPASIAELISATSMDLGPDGRDHEFGAGLVNAYAALVGATIDRAVFAIKDNAGK
                     WITDSVQGTRDRTFTIFSAPAGDHVLVGFLDVDGNGLISKGDFYGEAPITIPIAGTVY
                     PPRLVLNYVDGATATKRFTTSSRLP"
BASE COUNT         1129 a         1454 c         1580 g         1360 t
ORIGIN      
        1 catatggctc catagaagca tgctctgaga tgatgacgtg gatgtgggca aaaagacggt
       61 gggtatcgaa ggcggcacac ggtcctggtt tgcacgggcg cagcctgcgg tgatatcatg
      121 agcccctgaa gtgagcagac gagctgaggg tctgcttgat ctttcctgtg cgctcagcct
      181 gctcatcttc tgtgcccaaa tgccagcgtc cgatctttgg ccattggcaa ttgactagag
      241 ccgcaatgca ggctcatagc tccgaaatgc tgatctcgcc catggatgcg cggactttga
      301 gttctcctgt cggcgtgccg ttgccgagaa ttccggtggc agacgacttc tgattgtcgg
      361 agttcagacc tgtaatcggc agcgagctat ttatctgtcc ggcggaaatt gctgcgtcta
      421 ttcgcacaca cgtagaccta ggaacactga tcttgatggg acctgcagag ctctcaatgt
      481 aattgttctt tccgagagtt ccaaggaagt ctatgggccc cattgacgta atcaggctca
      541 acttgtttgc aacgactgcg tcacgcacgt ctatcgcgcc aacggagacc tcgatatcca
      601 aatcgcctct ggtggaatcg gtgttcacct tgcccgtatc tactttggcg tagatagccc
      661 catcgatccc attcagggtg atctcgcccg cagagagcgt ggacctaacg tctatccctg
      721 ctggaacctg aattaccatg ttgattctgc tggacgagcc cgttgtagcc gatgtgtcaa
      781 ccaggctgat tatctcgaga acatcatcgc gagtccgggc ttcgagcggc acaagtgaca
      841 gcttcgtctc tgctttttcc aatgtctcgc cgtaggcgat ccgttcgccg tctatagtga
      901 tgttgttgcc gcctgtcttg atgactgtaa tgtcgcctac gggtacctgt atagccacac
      961 acttgatgcc ggcggcgctg aaagcctcct caaactgtgc ttttgcaatg atgcctgtat
     1021 ccgtattgat gtaaggatcg aacatggatg aatacttatc tgcaagcagg ttgcttcctg
     1081 acacccatgg gtgggtggag ctcgctacta cagccagccc gataagaacg attataacta
     1141 tccctcgccg agaaatcatc tattatgtcc ctcctattta tgaagctcta tttgtcgatc
     1201 aggcgtcgac ttccaactca aggatcttgc cggcgatcaa gatgagaatt acggtgccca
     1261 tgatagtcgg ccagaacgga agcaacggta tgactttgcg agccgccatc ggtcctagcc
     1321 ccgtgtatac aatcagcccc gggttgggca cgaaggagaa agcgtacatc gcgtgcggtg
     1381 cgaacgcaaa ggtcaaatac catgtgaggc cgtagacgat cacagcgatc aaacctgaga
     1441 tgcgtggcac tatggttcct atcgtgaagg agaagagtcc caccgcaacg atgaacgcag
     1501 tgccaaggat cgaacatacc accatccata tgatgtcgag ggttctctcc cacggcgtca
     1561 aacgcaggat atcctctgtt gctgacatga tagtggagta gggtattgag gaccgttggg
     1621 tcacaaacca tgttccaact gttgcaacga cggtgatgag tcctaggccg agaacggcca
     1681 cgagcaactt ggcccagaga attccggcgc cgctcactgg atacgatagg agctgcagag
     1741 aggttcggtc cctccactca ttgcgcattg tgtagtatcc gactaccagg gcggctatgc
     1801 ccattcccac aaagggtatg aatgacagcc cgaaggcgag ccctccgttg attcttgccg
     1861 accatacggg cgccttgatc gcaagccacg tatcgaatcc gatggtagct gccaaggcca
     1921 gcaaaatgac ggtccaggag ccgcgccatt cctttcccac aagtgaccac caacgcttca
     1981 cttcaacaca cctccgcgaa gatttcattt aaggatttgc cccgttcggc tcttagctgg
     2041 tcggcttcgc ccgtcaagac aattcgtcct tcagagagga agatcacgtc ttcgaagagc
     2101 ttttccgatt caagaacttc gtgggtggat atgattatcg tctgctcgcc tgcgcgatac
     2161 ctgtcggcta tagtctcgag tatcgcggtg cgcgaaggcg ggtcgatgcc tgagagtggt
     2221 tcgtccatta gcaccagctc tgcgcggcga gacattgcaa ggaccagttt caacctgccg
     2281 cgctgtccct tcgagagcga cgagatcttg ttgtcgggat ccagtttcag ctctcgtaca
     2341 aggtccatgg cgtctgcgag gacgaagtcg tcgaacatag aactttcaaa ggcaattgcc
     2401 tgcctcaccg tcatccacga gtagaagctg tctatatccg ggatgtatgc ggttattgcc
     2461 ttcgttgcaa tggaaatgtc ttgcccgtgt acttgcactt ttcccgaggt cgggcttgca
     2521 agcccggcgc atatcctcag caaggtagac ttgccggatc cattggggcc caaaaggcct
     2581 acgatctttc cccggtccac gctgaggttt acgcctcgca gggcgtggga acggtaatat
     2641 cttttgtgga catcagtcag ctcaatagcg ggcatcactg aacaccccca tcatggtcat
     2701 ttgtagtgct tggaccgttt tgagcagctc cgttggagct attggttccg gttttggcca
     2761 gttgctgtga gacgagcgag attatgtcgc tgtcggagta gcgcaaccgc ctcatctctt
     2821 caacgaaaga ctccactgct gcagtggtca tctctgatct gacctccctc gctatttccg
     2881 ggctcgcgga gacgaaagtt ccttgacctc tttgagtttc caccactccc atcctctcga
     2941 gctctgcata tgcgcgttga accgtgttcg gattggtctt ggcctgtttg gcgaagtcgc
     3001 gcaccgacag tagcttgtcg ccgggaagca gggcgcctgt ggccacatcc cagagcacgc
     3061 tcttcattat ctgcgtgtag ataggtacag attcgtcgaa gctgatcccc aatcagatcc
     3121 ctcctttccg aagcgcttca tggcgctgtc atactgtact agtggactaa tacagtgaca
     3181 ctataatatg atgccgttgg aaggatgtca aggggttttg aggactattc tgggtggata
     3241 ataaagggtg cgttgaacga tggaactagg gcgtcgggct tgccgagagc gtcatgcgca
     3301 gataactaag gtgttcgttg cgactataca tcccgcgcca gcgctagttc gattatttgc
     3361 attcccgagg tcgccgttga caaccccacc tggggatgat aaaatcatgt tcgcaggaat
     3421 ttagatatgg cggcgtagct caggtggtca gagcatgcgg ttcatacccg cagtgtcact
     3481 ggttcgagtc cagtcgccgc taccatacga aattacagcg ccatcagggt ttgcctggcg
     3541 gcgctttttc gtttgcattg gcccgcttgt cgctgccgag aacagtcctg tttgatagtg
     3601 aaaaaatgca taggttaacc gccacgcctg gggtcgacat tcagggctct acggggccga
     3661 tccatgttag gaaggggatt gacgatggga tagctgttgt cgagtggagt gccaggtgcc
     3721 tgctgtgttc agagactgag accttgctat ggcaggcggc tagaagtggt gaagcgtttg
     3781 gtggcagttg ccccgtcaac gtagtttagg acgaggcgag gggggtacac cgtgcctgca
     3841 atggggatag tgatcggcgc ttccccgtag aaatctccct tgctgattag gccgttgcca
     3901 tcgacgtcca aaaagccaac gagcacgtga tcgccggctg gcgccgagaa gatagtgaat
     3961 gtgcggtcgc gcgtaccttg gacggaatcg gttatccact ttcctgcatt atccttgatg
     4021 gcgaagactg ccctgtcgat ggtcgcgccg actagagccg cgtaagcgtt gacgaggcct
     4081 gcaccgaatt cgtggtcgcg accatcgggg cctaggtcca tagaggtagc gcttatgagc
     4141 tccgcgatag aagccgggct catacgaccg tgggtcgcta ttaggagcgc tgcgatgccg
     4201 cttacatggg gtgttgccat ggacgttccc tggctgagga aatatttgtt gggtgccgga
     4261 tttgcgctac tgtatccggt gctcagcact gggaaaaggg cggtgccgcc gggcgcggcg
     4321 actgtgattt ccgggccata actggagtag ctagcaagtt cgttgttttg gccagtagcg
     4381 ctcacagcta ttacctccgg aaaggcagct ggatacagga ctggtgcgcc gctatcgttt
     4441 ccggctgccg ccaccattgt gactccacgt gagaaggcgt atcttactgc gtcttccatg
     4501 gctgagccgg ggtgattggc caggccaaga ctgaggttga tcacgtcagc tccactattg
     4561 accgcccata tgattgcctc tgccacgttg gacgagcttc cttttccatc ggggcccagc
     4621 actctaaggg gcatgatctg ggcgccccag cttactcccg ccacaccgac cccgttgttc
     4681 gttgcagctg cgatggtgcc tgatacgtgg gtgccatggc tgtatttctt cgtggaattg
     4741 ggccaatcag tgggatcaga atccttgtca atgaagtccc agcctgcgac tatcttgcct
     4801 tgcagatctt catgttcggg gcgtattcct gtgtcgatca ctgcaacgat gacggggctt
     4861 ccttcgccgg tggctatgtc ccatgcttcc gggaggctga ttgcagggta atgccattgg
     4921 tacaagtaca gggggtcatt gggaactgcc gcagcgtata cagggtaatc tgggtatgcc
     4981 caatctacgc catcgatgga gtcgagggtc gccgcttcgc tttcgacgag aaatcttgca
     5041 gtgcgttgca taggacgtat gaggatgatg cctgttaggg gcagttcatc gacgatctcg
     5101 tatccggctt ggagtaggtt atgtgcgagt tcgtcgcggt ctgtgccagg ccggactttc
     5161 acctggatgg tgtcggcttc tgtttccggc gcaggttgct ggcctagcgg ggccttttgg
     5221 aatgctgaca gtgaacgcga agctgtcttt gcgctgagaa catggttcga ctggtcctcc
     5281 tccgggtagt agttgtttgc aacggttgcg cagcccctga cagttgccag gcctgaggtg
     5341 ttcgggtata gttcgatctc gcagttgata ctgttgccgg ctggcacagt aacatttacc
     5401 tgtcttggcc tatagcctgg gcatgatgcg ttaaggatta ctcttcctgc aggcacattg
     5461 gacagggtga aggttccgtt gggcccggtg gcagcggacc ttttggggcc ggcgtccaca
     5521 gtt
//
LOCUS       DAOSAI010000137         5516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9309_length_5516_cov_3.432522, whole genome shotgun sequence.
ACCESSION   DAOSAI010000137 DAOSAI010000000
VERSION     DAOSAI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9309_length_5516_cov_3.432522"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /gene="efp"
                     /locus_tag="PLM83_05810"
     CDS             <1..405
                     /gene="efp"
                     /locus_tag="PLM83_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOK70955.1"
                     /translation="NTFRAGEKLPKAQIEYREMQFLYESGGQYFLMDQNTYDQISLSQ
                     DVLADAIQYLKENMIIGVQFYEGSPIGIELPNFVELEIVETDPGLRGDTASGGTKPAK
                     LETGAVVQVPLFVQTGDKIRVDTRTNQYLERV"
     gene            976..2067
                     /gene="spoIIIAA"
                     /locus_tag="PLM83_05815"
     CDS             976..2067
                     /gene="spoIIIAA"
                     /locus_tag="PLM83_05815"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HOK70956.1"
                     /translation="MRRIVRDLVAEQICPALPPRAREAVKALPDEVRQSLTEIRLRRG
                     RPAMGVVPEGDFYLSSDGQPLWLDRGRAAFSSEIPASAVMCSEDEWDAAMRLVTECSI
                     YALERELAAGYITLRGGHRVGLVGRAVLEGGRIRTQRELSAMNFRIARQIIGAADSIM
                     PYIVRRVGTLQHHRVSVFSTLILSGPGYGKTTLLRDIARQLSYGQNGTGLMVGVVDER
                     SEIAACSGGIPGNDLGPRADVIDSCPKAEGMMIMIRSMSPDVIVTDEIGHAADSEAIE
                     EARRCGVAVIATAHASDWDDAQRRPALRDILARGAFERGILLSNRLGICTCERIVHMA
                     TGTDLLDSPFLLSARADERDGGACRCGLR"
     gene            2052..2594
                     /locus_tag="PLM83_05820"
     CDS             2052..2594
                     /locus_tag="PLM83_05820"
                     /inference="COORDINATES: protein motif:HMM:NF021090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HOK70957.1"
                     /translation="MRVALKIAGAALTIISSGLAGLTVACSYRTHARELAGWVTALAV
                     LETEISYGLTKLGQAMRHAGQSAGGSPKRVLARAADLLEDGGYGSAGDCISKALTEET
                     ASCSFLWSGKWREDLEAVVVLGEALGASDSADQARHISLASRRLERRRQLAEEAATVN
                     YRLWSAMGFLIGGMVAIALI"
     gene            2630..2827
                     /gene="spoIIIAC"
                     /locus_tag="PLM83_05825"
     CDS             2630..2827
                     /gene="spoIIIAC"
                     /locus_tag="PLM83_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HOK70958.1"
                     /translation="MGLDLVLRIAGVGILASVLCTILKQQDKEEYSLLVATAGLVVVF
                     VMLLDYIEELLSSVRTVFRLW"
     gene            2843..3232
                     /gene="spoIIIAD"
                     /locus_tag="PLM83_05830"
     CDS             2843..3232
                     /gene="spoIIIAD"
                     /locus_tag="PLM83_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HOK70959.1"
                     /translation="MEIFQVVGMAILGAVLLAFLRQTRSDMAIVLSAALGIVVFLMVV
                     TRLAYFVEVLSDLAVRAGVNRMYLDTVLRVIGVAYLAAFGGQVCRDAGEGALAAKVEL
                     AGKVLILAMSVPLMFALLDLILRILPS"
     gene            3229..4635
                     /gene="spoIIIAE"
                     /locus_tag="PLM83_05835"
     CDS             3229..4635
                     /gene="spoIIIAE"
                     /locus_tag="PLM83_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HOK70960.1"
                     /translation="MMRLGLVKLPLVQGGDGGLAEEAIAGRTPGSAWIAGTVRMVKKG
                     KTSVRSGGAGQVGTGPKGMRERSSCSPAKVAVIIICFILCSHWAVAKCSGRGESGIAD
                     SPDSLLAEAAMEQISAMDVAEIERFIRGLDDDIAENMPAFNIIDIMSNQAAGIFDLGG
                     LARKAVSFLLREVKVNLKLLGELIVVAMIGSILCTFGSGLGEEGPCAMAWAVCYMTLV
                     TMGIRSFSVSADLATGAVDSMISFMHAIIPALVGLLATSGGVTTAAVVSPLVLVATTT
                     VGTLVKTTVIPLLLLGAALTLVGNVSDRKQVTKLASLLRWWSMTILGLASTLFVAFVG
                     VRGGLASVSDAASAKAAKFLAGSMIPVIGKVFGDAIDLVAASSLVVRGALGAFGLVSL
                     VVVCLFPVFKMVAVMLMFKIAAAVAEPLGDPRVSDCLGELADVLSALCICVTTVGLMF
                     FICISLVVGLSGLVVAIR"
     gene            4786..5331
                     /locus_tag="PLM83_05840"
     CDS             4786..5331
                     /locus_tag="PLM83_05840"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HOK70961.1"
                     /translation="MEAISAWVRGIVVLVLAVTFLEMLVPRSGLKKFVDMVMGLAVIA
                     GIIAPLVNIGMGYVDGKLAESDRFAMSGNLGSFENLGTLGEPHGLDGQQIAAELTETV
                     IEAKVREALAARFYGKNCFWEVRASIDRSGALLGIEVNVSFGGAAEGCARPGDSEIAA
                     AASAALGVDPEMIIVRQVGRR"
     gene            5328..>5516
                     /locus_tag="PLM83_05845"
     CDS             5328..>5516
                     /locus_tag="PLM83_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70962.1"
                     /translation="MSEERRFGTDLLKMPGLKPLLLLAGLGVLLIIVGSLVKTSPRDS
                     GNAVDGYSGLEPISSETSP"
BASE COUNT         1105 a         1362 c         1757 g         1292 t
ORIGIN      
        1 aatactttcc gggcgggtga gaaactgccg aaggcacaga tagagtacag ggagatgcag
       61 ttcttgtatg aatcgggcgg gcagtatttc ctgatggatc agaatactta tgaccagata
      121 tccctcagcc aagacgtatt ggccgacgcc atccagtatc tcaaagagaa catgatcata
      181 ggagttcaat tctacgaggg ctcgcccatc ggtatcgagc taccgaactt cgtggagctg
      241 gagatagtgg agacggatcc aggccttcgc ggcgatacgg cttcaggcgg aaccaagcct
      301 gccaagctgg agacaggcgc cgtggtgcaa gtgcccctgt tcgtgcagac aggcgacaag
      361 attagggtag atacccgcac caaccagtat ctggagcgcg tgtaagcgcg ccgggtggga
      421 cgtgcagggc gcgtttgggg cggattggtg tggtgcaaaa caatcaggtg tagaatgtgg
      481 cgaccgcaaa gcgcggtcgc ttccttatat gtcatcgaag attcgcccca aaagacggca
      541 cgtgcagtta tcgctcatat ctcggagcaa agctgatttg atcagcgcga aaaacaagag
      601 cgtgcttcag cccctacttc aatcttggac ttgtaaaact tgggtgtttg aggcggccgc
      661 ggccttgaaa ggttggctta ttaggagcgt tgtctgtact tttttagtgt atgcagtgta
      721 agatggccca tattgggtga ttccacgctg gaataataaa aaggcaggcg atgccatggt
      781 tgaatgtgag ctttcgcttt cgtcttcagg caacgcgttc agagcgtctg cctaattctc
      841 ttttcagact atgcacacac aagttctatc gcactcatgc gaaaatcggc atcctcgtcc
      901 ggtcatattc cttgcgccag cccatattat tgaatagcat gtctctgatt tgaaaccggt
      961 cttgggggga catttgtgag gcgcattgtt cgagatcttg tggcggaaca gatctgtccg
     1021 gctctccccc cacgtgcccg cgaggctgtt aaggctctgc ctgatgaggt cagacagagc
     1081 cttacagaga taaggctgag gcgtgggcgt cccgccatgg gagtggtgcc tgagggcgac
     1141 ttttacctct cctcagacgg acaaccctta tggctggacc gtggcagggc ggcctttagc
     1201 tcggagattc ccgcaagcgc ggtcatgtgc agtgaggatg agtgggacgc tgcgatgcgg
     1261 cttgtaacag aatgctccat atacgccctt gagagggagc tcgccgcggg ctacatcact
     1321 ctccgcgggg ggcacagggt ggggctcgtg ggccgtgctg tcctagaagg gggccgaatt
     1381 cggacgcagc gggagctttc ggccatgaat ttccggatag caaggcagat catcggcgca
     1441 gcagacagta tcatgcctta cattgttcgc cgcgtcggaa cgttacagca ccatcgggtt
     1501 tcagtcttca gcactctcat cctttccggg cccgggtacg gcaagacgac tctgctccgg
     1561 gatattgcac gacagctttc gtacggtcaa aacggaacgg gcctcatggt tggcgtcgtg
     1621 gacgagcgtt ccgagatcgc cgcatgctca ggcggcattc ctggaaacga cttgggccca
     1681 agagcggacg taatcgatag ctgcccgaag gccgaaggaa tgatgatcat gataaggtcg
     1741 atgtcgcccg atgtcatcgt tactgatgag ataggccatg ccgctgacag cgaggccatc
     1801 gaagaggcgc gcaggtgcgg cgtggctgtg attgccaccg cccacgcttc cgactgggat
     1861 gatgcgcaaa ggaggcccgc cctacgcgac attttggctc ggggcgcgtt tgaaagagga
     1921 atactcctca gcaacaggct tggaatctgc acgtgtgaac gcattgtcca catggcgaca
     1981 gggacagatc tcttggattc cccgtttctt ctttcggccc gggcggacga aagagacgga
     2041 ggtgcatgca gatgcgggtt gcgctgaaga tagccggagc ggcgcttacc ataatctcca
     2101 gcggccttgc tggactgact gtggcctgct cgtaccggac gcacgcgagg gagcttgccg
     2161 gctgggttac tgcccttgct gtgctggaga cggagatatc atatggcctg accaaactgg
     2221 gccaagccat gcgccacgcg ggacagtctg ctggcggaag ccccaaacgg gtcctcgccc
     2281 gcgcggctga tcttcttgag gatgggggtt acgggtcggc aggtgattgc atatcgaagg
     2341 ctttgaccga ggaaacggcg tcatgcagct ttctttggag tggcaaatgg cgcgaggatc
     2401 tggaagccgt agtagtgctc ggcgaggcgt tgggcgcctc cgactccgct gatcaggccc
     2461 gacatatctc ccttgcctct cgccgacttg agaggcgaag acagctcgca gaagaagccg
     2521 ccacagtcaa ttaccgtcta tggtcggcaa tggggttcct tataggcggc atggtggcca
     2581 tagccttgat ctgacgaacc ttgcgcacaa tgaaaaggag ggcctaacag tggggctcga
     2641 cttggtcctg aggatagcag gcgtaggcat tctcgcctcg gtgctgtgca cgatcctgaa
     2701 gcaacaggat aaagaggaat actcgttgct ggtagccacg gctgggctcg tggtggtctt
     2761 cgtcatgctc ttggattata tagaggaact gctttcaagc gtgcgcactg tattcagact
     2821 atggtaaggg gcggactgcg acgtggagat atttcaggtt gtgggcatgg cgatcttggg
     2881 agcggtcctc cttgcgttcc tgaggcagac tcggtccgac atggcaattg tcctttcggc
     2941 cgctctgggc atcgtcgtct tcctcatggt tgtaacgcgc ctagcctact tcgtagaagt
     3001 cctgtccgac ctcgcagtgc gtgcaggggt caacagaatg tacctcgata ctgttctgag
     3061 agtaattggt gtggcgtatc tcgcggcctt tggcggacag gtatgccgcg atgccgggga
     3121 gggggcttta gctgcaaaag tggagcttgc aggaaaagtc ctcatcctgg cgatgtctgt
     3181 cccattgatg tttgctttac tcgatctgat cctgaggatc cttccgtcat gatgcgccta
     3241 ggactggtga aactcccttt agtgcagggg ggagatggcg gcctcgcaga agaggcaatc
     3301 gcgggaagaa ctccaggatc ggcgtggata gccgggacgg tccggatggt gaaaaagggg
     3361 aaaacaagtg tgcgttctgg aggcgctggc caggttggga ctggcccgaa aggcatgcgg
     3421 gagcggagca gttgcagccc tgccaaggtc gccgtcatca taatctgctt tatcttgtgt
     3481 tcgcattggg ctgttgctaa atgctcgggt cgaggcgaat ccgggattgc cgatagcccc
     3541 gatagtcttc tggcagaagc tgcaatggaa cagatatcgg cgatggatgt tgccgagatt
     3601 gagcgcttca tccgcggcct cgatgatgat atagcggaga atatgcccgc atttaacata
     3661 atagatatta tgtcaaatca agcagcggga atcttcgacc ttggcgggct cgcgcggaaa
     3721 gccgtatcct ttctccttcg ggaagtcaaa gtcaatctga agctcctcgg ggagctgata
     3781 gtagtggcga tgatcggttc gatactgtgc actttcggat cgggattggg ggaggagggc
     3841 ccgtgtgcca tggcatgggc tgtatgctac atgaccctgg ttacaatggg aataagatct
     3901 ttttcggtgt ccgcagatct cgcgacaggc gctgtcgaca gcatgatctc ctttatgcat
     3961 gccataatcc cggcgttggt cggactcctt gcaacatctg gaggggtgac gaccgccgca
     4021 gtcgtttcac ctctggtgct cgtggccacc acgactgttg ggactttggt gaagacgaca
     4081 gtcatccctc tgttgctgct cggtgctgcg ctcacacttg ttgggaatgt gagcgaccga
     4141 aaacaggtga cgaaactggc cagccttttg cgctggtggt ccatgaccat tctgggcctc
     4201 gcgtcaacct tgttcgtggc gttcgtgggc gtgcgtggag ggctcgcttc tgtttcagat
     4261 gcggcgtcgg cgaaggccgc caagttcctc gcagggagta tgattccagt tattggcaag
     4321 gtgtttgggg acgcgataga tttggtcgct gcgtcttccc tagttgttag gggtgccttg
     4381 ggcgcctttg gcctagtttc gctcgtggtt gtctgcctgt ttcctgtttt caagatggtc
     4441 gcggtaatgc tcatgttcaa gattgcagca gcagtagccg aaccgctggg ggatccacgc
     4501 gtgtcagact gccttgggga gcttgcggat gtcctctccg ccttgtgcat ctgtgtgacc
     4561 acagtcggtt tgatgttctt catctgcata tccctcgttg tggggttgag tgggctcgtg
     4621 gtggcgatcc gctgactctt tttggcgtag gcgcgctgca agggagttag gttgttgcca
     4681 gggttcaagc tgcccttgag gctgcttggt gaatcagggc gttattaggc aacgtggcgg
     4741 gcgccgaatc aggtgccatg tcgatgcttt ggaggtggat ggcgcgtgga agccatatcc
     4801 gcgtgggtga gggggatcgt cgtcctggtg ttggcggtta ctttccttga aatgctcgtt
     4861 cccaggtcgg gcctgaaaaa gttcgtcgac atggttatgg gccttgcagt gatagcgggt
     4921 atcattgctc cattggtcaa catcggaatg ggatacgtcg atggcaagct ggcggagtca
     4981 gacaggtttg ccatgtcagg aaacttgggc tcatttgaaa acttggggac gctaggggag
     5041 ccacacggat tggacggcca acagatcgcc gcggaactta cggagaccgt tattgaggcg
     5101 aaggtgcgcg aagcgcttgc cgcccggttc tacggaaaga actgtttctg ggaagtccgc
     5161 gcatccatcg accgcagtgg cgcgctgctg gggatagaag tgaatgtgag ctttgggggc
     5221 gctgctgaag gttgtgcccg ccccggcgac tctgaaatag ctgccgctgc atccgccgcc
     5281 ttgggggttg acccagagat gatcattgta aggcaggtgg gaagaagatg agcgaagaga
     5341 gacgatttgg tacggatctg ctgaaaatgc cggggctcaa accactgctt ctcctggccg
     5401 gcttgggtgt gctattgatc atcgtgggga gccttgttaa gacttcaccg cgtgattccg
     5461 gcaatgcagt tgatggctat tctggcctag aaccgatttc ttcggaaaca tcgcct
//
LOCUS       DAOSAI010000138         5498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9357_length_5498_cov_4.588279, whole genome shotgun sequence.
ACCESSION   DAOSAI010000138 DAOSAI010000000
VERSION     DAOSAI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9357_length_5498_cov_4.588279"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PLM83_05850"
     CDS             <1..808
                     /locus_tag="PLM83_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70963.1"
                     /translation="IGPNGAGKTTTIRMLCGVVKPDSGQVRVAGLDPQSQGDEVRSQI
                     GVLTETAELYSNLSGIENLRFFAELYGIDDPRRPHELMEEFGLADHKDKKAGAYSTGM
                     KKRLGLAKALLANPPILLLDEPTSGLDPAGIAMVLDEIERLNSQKGVTVVLCSHVLHQ
                     LETVCQRYVIIDRGRTVAQGTLPELAEKCPGPTIVEVDADYILDGEAYRGIPARPLPV
                     GDIQFTVSSRNEVPKLIRQLSQDADVYSVKLKGHDLESVYFRLLEGEEDE"
     gene            801..1643
                     /locus_tag="PLM83_05855"
     CDS             801..1643
                     /locus_tag="PLM83_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOK70964.1"
                     /translation="MNRRAVRAIARKDIMSITSNIQVWLPMLIVPLVFAVLFPIGASF
                     AIRATDTSTSSDFQELLDVLEKLPQSSLDSLKGIFDSLITDQQRIAYMMFNYFMAPFF
                     LMIPLMAASTISVDSFVGEKERGTLEGLLLSPVDMRSIFAAKALASLIPAIAISLTSF
                     VLFAVCANVFAWPIFGRLCFPSIAWAPLILLVMPAIGLLAVLINVFISARVSTFQAGY
                     QMSGFLVLPVIALMIGQFTGIMLLDAKVLLAIGAALIIVDYALLMAITKRLDKAQIFE
                     SQVR"
     gene            complement(2165..3715)
                     /locus_tag="PLM83_05860"
     CDS             complement(2165..3715)
                     /locus_tag="PLM83_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOK70965.1"
                     /translation="MKSIRARLFLGIVGLVVGYVLLSWLLNSQLLDVYYHRIKTDSLV
                     SGYKHVKQAYDSGDLAQLLLDLETMERKEGLMVVIVDSSYTLQYASRPVQSARHGSAG
                     PTGPMDSALLDSLDQQRMPPPGPGPNPAPNPDPFPVNTIERAIADLEGEYEVLSFTRD
                     DRLKLDFISLIGRLPDGGYVFLSAPISSIEESASAANRFFLVSGALMIAVGSIAAYWY
                     AGRFTQPIHDMKVIAERVAKLDFSMEYVGSTDDEIGALGESINIMSRQLEYSINELVR
                     ANERLQQDIERERQIDEMRKEFIANVSHELKTPIALIQGYSEGLKLNVNDDEENRDFY
                     CDVITDEAARMNRLVGQLLKLAQIESGNIAPQKICFDIVEFINHIVRKNGLALQERGV
                     DLGIEGESCVEVVADIDMMEQVLVNYITNGIDHCDGERKMTITIEEVDEKARISVFNT
                     GQHIPEDCMDSIWTSFYKTDKARTRDHGGTGLGLSIVKAVQKAHGNDCGCKNVAGGVV
                     FWFDADLC"
     gene            complement(3712..4386)
                     /locus_tag="PLM83_05865"
     CDS             complement(3712..4386)
                     /locus_tag="PLM83_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOK70966.1"
                     /translation="MTKVLLADDEARMRRLVSDFLKREGFVILEAENGREALDLFLSD
                     QDIELVILDVMMPGMDGWVVCREIRRRSQVPIIMLTARAEESDQLFGFDIGADEYITE
                     PFSPMILVARAKALLRRTQAGDGGLRHLGLLEIDEGRHVVSVDGERLELSPKEFELLV
                     YFADNANLALSRDQILSSVWDYDYFGDARTVDTHVKRLRAKLGPAAGYIETVRGIGYR
                     FEATEE"
     gene            complement(4397..>5498)
                     /locus_tag="PLM83_05870"
     CDS             complement(4397..>5498)
                     /locus_tag="PLM83_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK70967.1"
                     /translation="VCWVKRNCMLKMVPVLLAVTLVLSGIALAGEYVDETLEVTLAQY
                     VEAYVGNSDAVKAAREDAENAQTQWQRAVDEKKAALTVKELENAAETKRLALDEAVNN
                     AALQAVQAYVGLGQALRELEAKRVSLAVSQERLRVTNLRYEAGLTTKDAVLEQENSYL
                     SASDSLLKAEQSLRQTKRSFCQNIGAELCDDIVLATDVSTLYGGVPEYGLNDSIEAAQ
                     GVGSSYFSAAAGRELARRKYEALQDPLIATPTERETAEKSWKDAETRFASAEQSLIDS
                     IENALVELESLVRNLNMQTISAQRAQSNLEVARIKFQHGEMLQYELDNETSSANQALY
                     RMAKAKEDLFVHMLKLESLIGGSVLDIVMCKK"
BASE COUNT         1249 a         1607 c         1288 g         1354 t
ORIGIN      
        1 catcggccct aacggcgcgg gcaaaaccac cacaatccgg atgctgtgcg gcgttgtaaa
       61 gcctgattca ggccaagtca gggttgcagg tcttgatccc caaagccagg gggatgaagt
      121 tcgttcccaa atcggcgtcc tcaccgagac tgccgagctc tattccaacc tctccgggat
      181 cgaaaacctc cgattctttg cggagttgta tggaatagac gatccccgaa gaccgcatga
      241 gctgatggaa gaattcgggc tcgcggatca caaagacaaa aaggccggtg cctacagcac
      301 tgggatgaag aaacgtctag ggcttgccaa ggccttgctc gccaatccgc cgatactcct
      361 tctggacgaa cccacatcag gcctggaccc ggcggggatc gccatggttc tcgatgagat
      421 cgagcgcctg aacagccaga aaggcgtgac ggtagtcctg tgctcccacg tcctgcacca
      481 gctggaaacg gtatgccaac gctacgtgat catcgaccgg ggccgcactg tggcccaggg
      541 aaccctgccc gagctggcgg agaaatgtcc cggccccacc attgtggaag tagatgccga
      601 ttacattctg gatggagaag cctaccgggg cattccagca agaccgctgc cggtcggcga
      661 cattcaattt accgtttcaa gcaggaatga agttcctaag ttgatccgtc aactttcgca
      721 agacgcagac gtctactcgg ttaagttgaa gggccatgac ctagaatcag tctatttcag
      781 gttattggag ggagaagaag atgaataggc gcgctgtgcg ggccattgcc cggaaagata
      841 tcatgagcat cacttccaac attcaagtgt ggctgcccat gctaatagtc cctctcgtct
      901 ttgctgtctt gttccctatt ggggcatcct tcgccataag ggcgaccgac accagcacct
      961 ccagtgattt tcaggagctg ctcgacgtgc ttgaaaagct gccccagtcg tcgcttgact
     1021 ccctcaaagg gatattcgac tcactcatca ccgatcagca gcgaatcgcc tacatgatgt
     1081 tcaactattt catggccccg tttttcctga tgataccgct catggccgcc agcacaatca
     1141 gtgtcgacag cttcgtgggt gaaaaggaaa gaggcactct ggaagggctt cttctgtctc
     1201 cagtcgacat gcggagcata tttgcggcaa aagctttggc atcacttatt cccgctatcg
     1261 ccatttccct cacttccttc gtgcttttcg cggtatgcgc gaacgtcttc gcctggccca
     1321 tatttggcag actttgcttc cctagcatcg cctgggctcc cctcatcctt ttggtaatgc
     1381 cggcaattgg gcttctcgcc gtgctcataa acgtcttcat cagcgcgcgc gtatctacgt
     1441 ttcaggctgg gtatcaaatg agcggttttc tcgttctgcc agtgattgcc ctgatgatcg
     1501 ggcagttcac cggcatcatg ctgctcgatg caaaagtgct gctggccatc ggagcagctc
     1561 tcatcatcgt cgactacgcc ctcttgatgg ccatcacgaa gagacttgac aaggcccaaa
     1621 tctttgagag ccaggtcaga tagtcgaaac cggatccacc cgttagcctg gacgccgcct
     1681 cactgagaaa atgcatccca ttccaagccc tacagggtaa gcaggggatg cagacctcaa
     1741 ataaaaagct agacagaggc ggcggtctat taaggtctaa agactcgata ggagttgcca
     1801 gtcttgacag atgattctgt agtgaaagaa gggaactggc ccgtttgtga acttatctat
     1861 cggcctcttc tcgtcgcaat gaagccgctt caagaagcat taaggaatga gcctgcttcc
     1921 gtattactgc acaagcccag cgcaaaatcc cattgccagg gaggaatgtg tagaacgcta
     1981 cggcagcggc tggcagatat gatcacaccc cgattcggca tcacgcgatt caactctcat
     2041 ccgcccgtat cctgtacccg gtcatgattg ctttatgtga gtgccatcat cattggtagc
     2101 tcaaatctcg cttctccttg aaactgttcg gagtgataat ggacgcaaca cctgtactcc
     2161 acagtcaaca caggtcggca tcgaaccaaa atactactcc gcccgcaacg ttcttacatc
     2221 cgcaatcatt gccgtgagcc ttttgcactg ctttcactat gctcagccca agccctgtcc
     2281 cgccatggtc acgtgtgcgc gccttgtcag tcttatagaa actagtccat attgaatcca
     2341 tgcaatcctc aggtatgtgt tgtcccgtat tgaaaacact gatccgcgct ttctcatcga
     2401 cctcctctat tgtgatggtc atcttgcgct ccccgtcgca atggtcgatg ccgttggtaa
     2461 tgtaattgac caacacctgc tccatcatgt cgatgtcggc cacaacctcc acacacgatt
     2521 cgccctctat ccccaagtca actcctcgtt cttgcaacgc caaaccgttc tttctgacga
     2581 tatggttgat gaactcaacg atgtcgaagc agattttttg aggtgcaata ttccccgatt
     2641 caatctgggc cagtttaagc agctggccta caagacgatt catcctggca gcttcgtccg
     2701 ttattacatc gcagtagaaa tccctattct cttcgtcatc gttgacattg agctttagtc
     2761 cttcagaata tccctgaatc aacgcaatgg gcgttttcag ctcgtgcgac acatttgcga
     2821 tgaattcctt gcgcatttcg tcgatctgac gttcgcgctc aatgtcctgc tgcaagcgtt
     2881 cattggcgcg caccagttcg tttatggagt actccagctg gcgcgacatg atgttgatgc
     2941 tctcgccgag agcgccgatc tcatcatcgg tgctgccgac atactccatg ctgaaatcga
     3001 gctttgccac gcgctcagca atgactttca tgtcatggat aggttgtgta aaccgtccgg
     3061 cgtaccaata ggcagcgata ctccctactg ctatcatcaa tgctccgctt accaaaaaga
     3121 acctattagc tgccgatgca ctctcctcaa tcgacgagat cggtgcgctg aggaatacat
     3181 aacccccatc aggcagcctt ccaattaggc ttatgaagtc cagcttaagc ctgtcgtcgc
     3241 gggtgaaact gaggacttcg tattcaccct ctagatcagc tatcgcgcgt tcgatcgtat
     3301 ttacggggaa cgggtccggg tttggagcgg gattaggccc agggcctggc ggcggcattc
     3361 tttgctgatc gagcgaatcg agtagtgcag aatccatagg gcccgtcgga cccgcagagc
     3421 cgtgcctggc ggattggaca ggcctcgacg cgtactggag cgtataggag gaatccacta
     3481 taaccaccat caggccctcc ttgcgctcca ttgtctccag atccagcaac aactgggcca
     3541 agtcgccact atcgtatgcc tgcttcacat gcttgtagcc gcttacgagt gaatcggtct
     3601 tgatacggtg atagtacaca tccagcaact gcgagttgag caaccaggag agtaatacgt
     3661 agcctacgac caggccaacg attcccagga ataagcgtgc tcttatagac ttcattcttc
     3721 agtcgcctca aatctgtagc ctatgccacg tacagtctca atatagcctg ctgcagggcc
     3781 cagcttggcc cgaagacgtt tcacatgagt atccactgtc ctggcatccc cgaaatagtc
     3841 gtagtcccac acagagctga gtatctggtc ccgcgacaag gctaggttgg cattgtcggc
     3901 aaagtagacc agaagctcga attccttggg gctcagctcc agtctttcac cgtccacgct
     3961 caccacgtgc ctgccttcat ctatctccag caatccaaga tgcctgagtc ctccatcgcc
     4021 cgcctgcgtg cgtctgagca aggctttagc cctagctacg agtatcatgg ggctgaaggg
     4081 ttctgtaatg tactcatcag ccccaatatc gaagccgaag agttgatcac tctcttcggc
     4141 tcttgctgta agcattatta tcggcacctg tgatctcctg cgtatctcgc ggcagaccac
     4201 ccaaccatcc atgcccggca tcattacgtc caatatcacg agctctatgt cttggtcgct
     4261 gaggaaaaga tccaaagcct cccggccatt ctcagcttca aggatgacaa agccctcccg
     4321 tttgagaaaa tccgatacga gcctgcgcat tcttgcctca tcatccgcca gaagaacttt
     4381 ggtcacgaat ctcgcctcat ttcttgcaca tcacgatgtc caggacgctg ccgcctatga
     4441 gcgattccaa cttcaacata tgtacaaaca ggtcttcctt tgctttggcc attctgtata
     4501 gtgcctgatt agctgagctt gtctcgttat cgagttcata ctggagcatc tctccgtgtt
     4561 gaaacttgat tctggcaacc tctaggttgc tctgagcgcg ctgcgcggag atggtctgca
     4621 tgttcaagtt tctgaccagc gattccagct ccaccagagc attttctatg gaatctatca
     4681 aagactgctc ggcgctggca aatcgggtct cggcgtcctt ccatgacttt tccgctgtct
     4741 cacgctccgt aggcgtagca atcagcggat cttgaagcgc ctcatacttc cttcttgcca
     4801 attccctgcc tgcggccgct gaaaagtatg aggaaccaac tccctgcgca gcctcgatag
     4861 agtcgttaag gccgtactcg ggcacgccgc catataatgt actaacgtca gtggccagca
     4921 caatatcatc acaaagttca gctccgatgt tctggcagaa actccgtttc gtctgcctca
     4981 ggctctgctc tgcctttaaa agcgaatcag aagctgaaag atacgagttt tcctgttcta
     5041 atacggcatc tttggtagta agccctgctt cataccgcaa attcgtaact cgtagccttt
     5101 cctgtgacac agccaaagaa acgcgtttgg cctccagctc ccttagagcc tgtccgagtc
     5161 ctacataggc ctgaacagcc tgcaaagctg cattgttcac cgcctcatcc aaagcgagtc
     5221 gcttagtctc ggcagcattc tcaagctctt tcacagtcaa tgcagccttc ttctcatcaa
     5281 cagcccgctg ccactgggtc tgcgcattct cagcatcttc ccgagctgcc ttcactgcat
     5341 cgctgtttcc cacatatgcc tcgacatact gagccaaagt cacctccagt gtttcatcta
     5401 catattcccc cgcgagcgca atgccgctta acaccaacgt tactgccagc aagacgggta
     5461 ccattttcaa catgcaattt cgtttcaccc aacacacc
//
LOCUS       DAOSAI010000139         5492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9367_length_5492_cov_3.483723, whole genome shotgun sequence.
ACCESSION   DAOSAI010000139 DAOSAI010000000
VERSION     DAOSAI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9367_length_5492_cov_3.483723"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLM83_05875"
     CDS             <1..845
                     /locus_tag="PLM83_05875"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOK70968.1"
                     /translation="VLADADLWSRRRKVVEHTLRGRAGSADEEASPEGKRRAVDPPRP
                     ELLSMSFSVREVNLPIPTEDEIREILAQTDKFSPEDELNCGACGYNSCRDKAIAVYRG
                     MAEVEMCIPYMRRRAESMSNLIISSTPNAIVAVDRDLRIVLVNPAFESVFRLSAESCV
                     GQHVSVAMDPEPFRRVFETKELISIEEEHLGGELRTSQIVFYVEVRDLVVAIGTDITA
                     EYDTARAIQKAREETLEKAGAVIDRQMRVAQEIAGLLGETTAETKLLLTQLIKQMQDE
                     ELTR"
     gene            845..1987
                     /locus_tag="PLM83_05880"
     CDS             845..1987
                     /locus_tag="PLM83_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOK70969.1"
                     /translation="MSELHVEAAFTSLPKHGEELCGDRVEIARSGDMVTVVLSDGLGS
                     GVKANILATLTSTIAVSMLRGGASIDDVVETIGHTLPICQVRKVAYSTLTILQIKNDG
                     SAYLVQYDNPDAFYYSDGMIVPIESSDRIVADKVIKEARFQLKAGDRLFLVSDGVIYA
                     GVGGVLNLGWGWPNVADYIKKINDQESRASVLAKWLVNVCDQFYAGQPGDDTTAVAVV
                     IREPRCLTVAVGPPKKPEDDRKMVARLMSSVGTKVVCGGTTGKIVAREIGESLHVDLD
                     TIHGGMPPTASINGIDLVTEGIITLSKTLDRLIQGEPVHGKDGASRLARMLLDADHVE
                     LLVGKAVNPAHQNPDLPISTTLKERVMDDIARILVELGKEVSVSYY"
     gene            2046..2543
                     /locus_tag="PLM83_05885"
     CDS             2046..2543
                     /locus_tag="PLM83_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOK70970.1"
                     /translation="MTAVEAAQRRFERVNEIIEKHGRSQSSLVPILQDVQAEYRYLPE
                     EILTYVATAMGLSVATVYGVATFYAQFSLEPKGKYVVRVCDGTACHVRQSMPVYEAVR
                     SKLELADGKFTTPDLMFTVETVSCLGACGLAPVMTINDEVHGKMTPEAACIIIDTLLE
                     REGQI"
     gene            2546..4381
                     /gene="nuoF"
                     /locus_tag="PLM83_05890"
     CDS             2546..4381
                     /gene="nuoF"
                     /locus_tag="PLM83_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOK70971.1"
                     /translation="MAITSREELNACADQFRKELASKKKRVLVCAGTGCLANGSAKVL
                     QEFERLSVDAVGVTKSGCHGFCEAGPLVRIEPDHILYVHVKPEDVEEIVEKTLKLGEP
                     VERLLYHHPVTGKAYLGEGDIPFYKSQTRVALANCGAIDPDDIREYIALGGYKALARA
                     VVDLTPEEIIEEILESGLRGRGGGGFPTGRKWMFAQAAEGSEKYIVCNGDEGDPGAFM
                     DRSVMEGDPHRVIEGLAIAARAIGASHGYVYVRAEYPLAVARLQNAVKQARELGLLGE
                     GIFGTEFAFDVTIKEGAGAFVCGEETALLASIEGKRGMPRPRPPFPAHEGLYGKPTVI
                     NNVETLATVPPIMLHGPSWFRSIGGETSPGTKTFAITGQVANTGLIEVPMGATLREVV
                     FDIGGGVRTGRAFKAAQIGGPSGGCLPAELLDTPLEYDALAKVGAMVGSGGLVIMDEG
                     TCMVEVARFFMKFTQHESCGKCVPCREGTKRMLEILEKIVAGRATMEDLDNLEEIAWV
                     VKESSLCGLGKTAPNPVLTTLRYFRDEYVAHIVDKECPAGVCVAFKHYSIDPELCKGC
                     GKCKRSCPAGAISGEVKNPHVIDVDKCIKCGACAGNCPFGAISLR"
     gene            4402..>5492
                     /locus_tag="PLM83_05895"
     CDS             4402..>5492
                     /locus_tag="PLM83_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOK70972.1"
                     /translation="MSAQQMVTIDGLAVPIEGERNLLELIRKAGIDLPTFCYHSELSV
                     YGACRMCVVEIENRGVVASCTVPPQPGMVVTTNTEKLRTIRRTIIELLLANHDRDCTT
                     CSKNGACMLQDLARRFGVTNVRFGQADERRRLDRSTPAIVRDPNKCILCGDCVRVCGE
                     VQGIGVLDFAYRGSYSMVTPAFGKELADVDCVQCGQCASVCPTAAITIHSSTDEVWKA
                     IHDKNKTVVAQVAPAVRVALGEEFGHAPGWAEPGKVVAALKKIGFDLVFDTSFTADLT
                     VVEETAEFLERISSGNEGRLPQFTSCCPAWVKYVEQYHPEYLSNLSSCRSPQQMFGAL
                     ARTYLPAQLRVEPEEIYVVSVMPCTAKKF"
BASE COUNT         1257 a         1281 c         1751 g         1203 t
ORIGIN      
        1 gcgttctggc cgatgcggac ttgtggtcta ggcggcgtaa ggtagtggaa cacactctgc
       61 gggggcgtgc aggcagcgct gatgaggagg catcgcccga aggcaagcga agggctgttg
      121 atccgccaag gccagaattg ctcagcatgt cttttagcgt gcgcgaagtc aatctgccca
      181 taccgactga ggacgagatc cgggaaatcc tcgctcagac agacaagttc tctcccgaag
      241 acgagctcaa ctgcggagca tgcggttaca actcatgtcg cgacaaggct atagcggtct
      301 atcgaggcat ggctgaagtt gagatgtgca ttccctacat gcgccggcgc gcggagtcta
      361 tgtctaacct gataatcagc tctactccaa atgcaatagt cgctgtggat cgggacctgc
      421 ggatagtcct ggtcaatccg gcgtttgaat ccgttttccg tctcagtgcg gagtcatgtg
      481 tgggacagca cgtcagtgta gccatggatc cggagccttt ccgcagggtt ttcgagacca
      541 aggaattgat aagcatcgag gaggagcatt tagggggcga gctgcgaaca tcgcagatcg
      601 tattctacgt tgaagtgcgc gacctggttg tggcgatcgg aaccgacata actgctgaat
      661 acgatacggc gcgggcgata cagaaagctc gagaggagac gttggagaag gctggagcgg
      721 ttatcgacag gcagatgcga gttgcgcagg agattgcggg gcttctgggg gagacgaccg
      781 ctgagactaa attgttgctt acgcaattga tcaagcagat gcaggatgag gagttgacgc
      841 gctaatgagt gaacttcatg ttgaggctgc gtttacttct ctgcccaagc atggggagga
      901 gctgtgcggc gacagagtgg agattgcccg cagtggcgac atggtcaccg tcgtgctcag
      961 cgatggcctc ggatcgggcg taaaagccaa catcctggcg actctcacat ctactatagc
     1021 ggtgagcatg cttcgcggcg gcgcgtccat tgatgatgtt gtagaaacta taggccacac
     1081 attgcccata tgccaggtgc gtaaggtggc ttactcaaca ctgacgatat tgcagataaa
     1141 gaatgacggc agtgcttatc ttgtgcagta cgacaatcca gacgctttct actattcaga
     1201 tggaatgatt gttccaatag agtcgtctga cagaatagtc gctgacaaag tgataaaaga
     1261 ggctcgattt cagctaaagg ctggcgacag gttattcctc gtctcggacg gggtgatcta
     1321 tgccggagta gggggggtcc tcaacctcgg ttggggctgg cccaatgttg cagactacat
     1381 caagaaaatc aacgatcagg agagcagagc atcagtgctt gcaaaatggc ttgtgaacgt
     1441 gtgtgatcag ttttatgccg ggcagcctgg agatgacacc actgcggtcg ctgtagtcat
     1501 tcgtgaaccg cgctgtctca cggtggcggt cggtcccccg aagaagcccg aggatgaccg
     1561 gaaaatggtg gccagattga tgagttcggt tgggaccaag gtagtgtgcg gagggactac
     1621 aggcaagatt gttgcgaggg agatcgggga atccctccac gtcgatcttg acactatcca
     1681 tggaggcatg cctccgactg cgagcatcaa tgggatcgat ttggttacag aggggataat
     1741 aacgctttca aagacgttgg atcgccttat tcagggtgaa cctgttcatg gtaaggatgg
     1801 cgctagcagg ctggcgcgaa tgttgctcga tgccgaccat gtggaattgc tggtgggtaa
     1861 ggctgtcaac cctgctcatc aaaaccccga tttgccgata agcactacac taaaggaacg
     1921 ggtgatggat gatatagcga ggattcttgt tgagcttggc aaggaggttt cggtttctta
     1981 ctattgagcg ccgattgctg ggcgctgaag cgggacagaa aaaatctttg tctggaggca
     2041 ggagaatgac agccgttgag gcagcgcaga gaagatttga acgtgtaaat gagataatcg
     2101 agaagcacgg tcgcagccag tcttcgcttg ttccaatact ccaggatgtg caggcggaat
     2161 acaggtatct ccctgaggag attcttacgt acgtcgctac agcgatgggt ctgtctgtcg
     2221 caacagtgta cggtgtagcc accttctatg cccagttttc cttggagccc aagggcaagt
     2281 acgtggtgag ggtgtgcgac ggcacggcgt gccacgtcag acagtcaatg cctgtgtacg
     2341 aggctgttag atcaaagctt gagcttgctg acggaaaatt cacaactccc gacctgatgt
     2401 ttaccgtaga aaccgtatcg tgcttgggcg catgtgggtt ggcgcctgtt atgacgatca
     2461 atgatgaagt ccacggcaag atgactccag aggcagcatg catcataata gacactctgc
     2521 tagaaaggga gggccagatc tagaaatggc gattacgtcg agggaagaac tgaacgcctg
     2581 tgctgaccag ttccgcaagg aacttgcgtc caagaagaaa cgagtacttg tctgcgcggg
     2641 cacggggtgt ctggccaacg gctccgccaa ggttttgcag gagtttgaaa ggctttcggt
     2701 tgatgccgta ggtgtcacaa aaagcgggtg tcatggcttt tgtgaagcgg ggcctttggt
     2761 gaggatcgag cccgaccaca tactgtatgt acacgtgaag cctgaggatg tagaggaaat
     2821 agtcgagaag actctaaagc tgggggagcc ggtggagcgt ctgctctatc accatccggt
     2881 taccgggaaa gcgtatctag gcgagggcga catacctttt tacaagagcc aaaccagggt
     2941 tgctttggca aattgcgggg ccatcgaccc cgatgacata agagaataca tcgctcttgg
     3001 cgggtacaag gcccttgcca gggctgtggt agatcttacg cctgaggaga tcatcgaaga
     3061 gatcctcgaa agcgggctca gagggcgtgg gggaggaggg ttccccactg ggcggaaatg
     3121 gatgtttgcg caggccgccg agggcagcga gaagtatata gtctgcaatg gcgacgaggg
     3181 agatcccggc gctttcatgg accgaagcgt catggaaggt gatcctcacc gagttataga
     3241 gggcttggcc attgctgcaa gggcgattgg cgcatcccac ggctacgtct atgtcagggc
     3301 tgaatatccg ttggctgtgg cgcgcttgca gaacgcggtg aaacaagcta gagaactggg
     3361 cttgctagga gaggggatat ttgggaccga atttgccttc gatgtgacta tcaaggaggg
     3421 cgccggcgct tttgtgtgcg gagaagagac ggctctccta gcatccatcg aggggaaacg
     3481 aggcatgccc cgaccccgtc cgccctttcc tgcgcacgag ggcctgtatg gtaagcctac
     3541 tgtcataaat aacgttgaga ctttggcaac tgtccctcct atcatgctcc atgggccttc
     3601 atggttccgc agcatcgggg gggagacgag tccggggacg aagacatttg ccataacagg
     3661 ccaggtggcc aataccggac tgatcgaggt tccgatgggt gcgactttga gggaagtcgt
     3721 cttcgacatc ggcggtggcg tgcggactgg ccgggcattc aaggctgcgc agataggcgg
     3781 gccatcagga gggtgcctgc cggctgagct acttgatact ccactcgaat acgacgctct
     3841 ggcaaaggtc ggcgcgatgg taggttccgg cggcctggtg atcatggatg aaggaacctg
     3901 tatggttgag gtggcacgct ttttcatgaa gttcacgcaa cacgaatcct gcgggaagtg
     3961 tgtgccgtgt cgtgagggaa ccaagaggat gcttgagatc ctcgagaaaa tcgtggccgg
     4021 gcgtgcaacc atggaggacc ttgataactt ggaggagatc gcgtgggtcg tcaaggagag
     4081 ttccttgtgc ggcctgggca agactgctcc gaatccggtc cttaccacgt tgcggtattt
     4141 ccgcgatgaa tacgttgctc atatagtgga caaagaatgt ccagccggcg tgtgcgtggc
     4201 ctttaagcat tattccatcg acccggagct gtgcaaaggc tgcggcaagt gcaagaggtc
     4261 gtgccctgcc ggggccatca gcggcgaggt gaagaatccc cacgttatag atgtggataa
     4321 gtgtatcaag tgtggagcgt gcgctggaaa ttgtccgttc ggtgctatct cactgcgatg
     4381 accgggggag ggcggaaatc aatgtctgca caacagatgg taacaataga cggtctggcc
     4441 gttcctatag agggcgagcg caatctgctg gagctcatac gcaaggcggg aatagatctt
     4501 cctacattct gctaccactc cgagctctcc gtttacggag catgcaggat gtgtgtggtg
     4561 gagatcgaga acagaggggt agtggcgtcc tgcacggtgc cgcctcagcc cggcatggtg
     4621 gtgaccacca acaccgagaa gctgaggaca ataaggagga cgataattga gcttctcctg
     4681 gctaaccatg accgtgactg cactacgtgt agcaagaacg gggcgtgcat gcttcaggac
     4741 ctggccaggc gtttcggcgt gacgaacgtt aggtttggac aagcagacga gaggcgtcgc
     4801 ctcgacaggt ctacgccggc tatagtgcgc gacccgaata agtgcattct ctgtggagac
     4861 tgcgtgcgtg tatgcggcga ggtgcaaggc ataggggttt tggacttcgc ataccgcggt
     4921 tcatattcca tggtgacgcc agctttcgga aaagagctgg ctgatgtaga ctgtgtccag
     4981 tgtgggcagt gcgccagtgt atgtccgacc gccgcaataa ctatccactc gtccacagat
     5041 gaggtgtgga aagcaataca tgacaagaat aagacggtgg tggctcaggt tgccccggct
     5101 gtgagagtag cccttggaga agagtttggg catgcgccag gctgggctga acctggcaag
     5161 gttgtggctg ccttgaagaa gatcgggttc gatctcgtct tcgacacttc attcactgcc
     5221 gaccttacgg ttgtggagga gaccgcggag ttcttggaga ggatctcatc gggcaacgag
     5281 gggcgtttgc cgcagtttac ttcatgttgc cctgcatggg tgaaatatgt cgagcagtat
     5341 cacccggagt atctttcgaa cctatcgtca tgcaggtcgc cacagcagat gttcggcgcc
     5401 ttggcccgga catatctccc ggcccaactg cgtgtggagc ccgaggagat atacgtggtt
     5461 tcggtgatgc cgtgcacggc gaagaagttc ga
//
LOCUS       DAOSAI010000140         5472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9400_length_5472_cov_3.670666, whole genome shotgun sequence.
ACCESSION   DAOSAI010000140 DAOSAI010000000
VERSION     DAOSAI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9400_length_5472_cov_3.670666"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="PLM83_05900"
     CDS             <1..1561
                     /locus_tag="PLM83_05900"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOK70973.1"
                     /translation="GRLSKAYQASLEMAAMEHERARKRDRWDRTLADLARAINSGLDL
                     HAVLEAVVDGGLDVLEAEAGMIAVVDKQGRTVAEACRALPPDFCGSVLEPGRGAIGLA
                     IESRKTVGILDYQKFDRALPEVRDMGLASVVAAPILMGSDVIGGLAFGHTVLGMTCTE
                     EECEFLEILARQLGVVLGNARFLEEARRRADYLSTINEISKSFASVLEPEVLFEKIYR
                     EVRRIVPMEAFFVAIYVPQRREVEIVFLVDDGKRCPPTRFKLDEGPTSRVIMSGQPFV
                     IQLEDEDDIEDAKWMGHKPDPTRSGIIVPMRVGPHVIGALSAQSYTPDAYGEEEIELL
                     TTIAASAAIALENARLYQTARELSLTDDLTDLGNYRFFCNALEREIERAKRNGGPLSL
                     VMMDSDSLKYINDRYGHAIGDMHLIHLAETMKAISRKSDILARYAGDEFMIILPNTKK
                     EDASVMAERLRTRIENSPLSVDGSLVVATVSVGVASYPEAGSTVDELVRAVDAAMYKA
                     KRQGKNRVVCV"
     gene            complement(1594..2502)
                     /locus_tag="PLM83_05905"
     CDS             complement(1594..2502)
                     /locus_tag="PLM83_05905"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOK70974.1"
                     /translation="MERTPVAIVGYGKVGRAAADAIAASPDFELTGIVRRKVVSDELI
                     RGRNWAAPAVTDISHLGGVQAALMCVPTRELERMEHGLLRSGISVVDAFDIHGDAICQ
                     HKEALHSSAIAGRAVAVIASGWDPGIDSLIRALMELMAPRGITYTNFGPGMSMGHSVV
                     ARSVAGVRDAISITLPAGYGKHRRTVYVEEDGSRPFQEIVRDITRDPYFSDDDTEVFS
                     VASVAPLADSAHAVEISRKGTAGEASNQLLEWQMKISNPAVTAQAMLGGLRAALKSEP
                     GAYTLLELPVAQMLPGNPKYNIKRLT"
     gene            2642..5263
                     /locus_tag="PLM83_05910"
     CDS             2642..5263
                     /locus_tag="PLM83_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOK70975.1"
                     /translation="MQVIVERAERCRRCHVCVTACPVGAIVISSGEVRLRRDLCIACG
                     QCVKACPNGALAIRDQGEDVMALIGNGRTVAILDPSFPGAFPQSDPLCLIGALRELGF
                     SSIWEASFGAESLLPYYADYVKAHSEESGRSVAFASMCPAVVAFIEHCRPELAGNLVP
                     LVSPTVAASRVARALGEKRVVFIGPCPAEKLEAGFGGVPGGPNIAITYAELSRLLDNV
                     GVSVDQCPASLPDVRASGAGRMLGLPAGLLRALRIRDDVAPPGILLAEGKEECLKLVE
                     ASLSGALGGSFVVASICRSCVDGTGFPEGASLWDRRRAVVDYIKEASSVGSMEVDGAL
                     RAVSASADFKRRYLPRKIPLAPKETDVRTALLGMGMRSPEDELDCGLCGFQSCRERAI
                     AVARGTTEASECFPRRNARLSEECARLSQLASDLDSIVNTSCEISYVCDPDGWIVRAS
                     RAANDIIGVENANLHKVNMKNLPRRLCPDVAIQALHEVKRVMAIRDLPGGHRLLAAAS
                     PILDGDGACRGVAATITDLSGAALEINTTPTASSFATDADSEDMVAYSPAMEELLTLV
                     HKVANVSSTVLITGESGVGKEVVARYIHRTGNRASGPFVKINCAAIPHTLLESELFGY
                     EEGAFTGARKSGKPGLIQLASGGTLFLDEISEMPLGIQAKLLQVLQEKCLVRVGGTEQ
                     IRVDARIMAATNRDLRAAVKEGRFRGDLYFRLNVIPIRVPPLRERSGDIAPLTYHFIE
                     KWDSRYGRNHQISREACSLLECYRWPGNVRELENLIEFLVVTVDDPVILPEHLPQHVV
                     AGEGERDVQVSVSGIMPLRQAVEEVERQLIALAAEACSTTYSMAKALGVNQSTVVRKM
                     QKYMETPEEEAEETGNV"
BASE COUNT         1204 a         1282 c         1664 g         1322 t
ORIGIN      
        1 agggcggctt tccaaggcct atcaggcctc attggagatg gcggctatgg agcatgagag
       61 ggcacgcaag cgggaccgct gggatcgcac tttagctgat ctcgcccggg caatcaattc
      121 agggctggac ctgcatgccg tgttggaagc ggtggtcgat ggcggcctcg atgttctcga
      181 agctgaggct gggatgatcg ctgtcgttga caagcagggt cgaactgtgg cagaagcttg
      241 tcgcgctctg cctccagatt tttgcggctc tgtgctcgaa ccagggcggg gtgcaatcgg
      301 acttgccatt gagtctcgga agactgttgg catattggat tatcagaagt tcgatagagc
      361 tctgcctgaa gtacgcgaca tggggcttgc ctctgttgtg gctgctccga tattgatggg
      421 ctcagatgtg attggcggtc tagcgtttgg ccataccgtg ctgggtatga cctgcaccga
      481 agaggaatgc gagtttctgg agattctggc caggcagttg ggcgttgttc tgggaaacgc
      541 gcgtttcttg gaggaagcca ggcggagggc ggattacctg tccacaatca atgagatatc
      601 aaagagcttc gcttcggtat tagagcccga ggttctcttc gagaagatct atcgtgaagt
      661 gcgccgcatt gttcctatgg aagcgttctt cgtagccata tatgttcctc agaggcgaga
      721 agtagagata gttttcttgg tggacgacgg aaagcggtgt ccgcctacgc gtttcaagtt
      781 ggatgaagga cctacttcga gagtcataat gtctgggcag ccattcgtca ttcagctgga
      841 ggatgaggat gacatcgagg acgccaaatg gatgggacac aaacctgacc cgacgcgatc
      901 cgggattatt gtgcctatga gggtagggcc acacgttata ggcgctttat ctgcgcaaag
      961 ctacacgccg gatgcgtacg gtgaagagga gatagagctc ctcaccacga ttgcggcgag
     1021 cgccgcaata gctttagaaa acgcacggct ttaccaaacg gcgcgcgagc tttcgctcac
     1081 cgatgacctt accgatcttg ggaactacag gtttttctgc aatgcattgg aaagagagat
     1141 cgagagagct aaacgtaatg ggggtccgtt gtccctggta atgatggact ctgacagcct
     1201 gaagtacata aacgataggt atggacacgc cattggcgac atgcacttga tacacctggc
     1261 tgaaacgatg aaggcaatat cgcgcaagtc tgatattctg gcgagatatg cgggcgatga
     1321 gttcatgata atactcccca atactaagaa ggaagatgct agtgtaatgg ccgaacgcct
     1381 tcggacgcgg atcgagaatt cgccgctgtc tgttgacggg tcgctggtgg ttgcaactgt
     1441 gagcgttggg gtcgcttctt acccggaggc gggatccact gtggacgaac ttgtcagagc
     1501 tgtggatgcc gccatgtaca aggcgaaacg gcaggggaag aacagggtgg tttgtgtctg
     1561 accctgtcgt gatcttatcg ttatgattgg ctactaggtc agccttttta tgttgtattt
     1621 gggattgccc ggcagcatct gggcaacagg aagttcgagg agagtatatg ctcctggttc
     1681 gcttttgagt gcggctctga ggcctcccaa catggcttgt gcagtgacgg ctgggtttga
     1741 tatcttcatc tgccattcaa ggagctgatt ggatgcttcc cctgcggtcc cctttctgct
     1801 gatttctact gcgtgtgcgc tgtctgctag gggcgccaca gacgcaacgg agaagacctc
     1861 tgtgtcatca tctgagaaat atggatctcg ggtgatgtct ctgacaatct cctggaaagg
     1921 cctggaaccg tcttcttcga cgtagaccgt tcgcctgtgt ttgccgtatc ctgcaggcaa
     1981 tgtgattgag atcgcgtcac gcaccccggc cacagaacgt gcgacgactg agtggcccat
     2041 gctcatgccc gggccgaagt tcgtatatgt tattccccga ggcgccatca attccataag
     2101 ggcccttata agagaatcga tgccggggtc ccagccggag gctatcaccg caaccgctct
     2161 gcctgcaatc gccgaggagt gcagggcttc tttgtgctgg cagatggcat ctccgtgtat
     2221 gtcaaaggcg tcgaccacgc ttatccctga ccggagaagc ccatgctcca ttctctccag
     2281 ctcccgtgtg ggcacgcaca tcagcgcagc ttgaactcct ccaagatgcg aaatgtcggt
     2341 gacggctggg gcagcccagt tcctgccgcg aatcagctca tcggagacga cctttcgcct
     2401 tacgattcca gtcaactcga agtccggtga agccgcaata gcgtcggctg ccgctcttcc
     2461 cactttccca taacctacga tggcgaccgg tgtgcgttcc atttaggcag gaagcctcct
     2521 ttaaaattgc ttggcttaac aggattatat agctgacagc gtgcgccgtc cagattacat
     2581 gatataatga acctgtgcat gatgcagttg cgcattaaca gacggattcg ggggactgta
     2641 attgcaggtc atcgtagaac gggctgagag atgtagaaga tgtcatgttt gtgttacggc
     2701 gtgccctgtt ggggctatag tcatcagcag tggcgaggtt cggttgaggc gcgacctgtg
     2761 catcgcctgt gggcaatgtg tgaaggcctg tcccaacggc gcattggcca ttagggatca
     2821 gggcgaggat gtaatggccc tcatagggaa tggacgcact gtcgcgatat tggatcctag
     2881 ttttccgggg gcttttcctc aatccgatcc gctctgccta attggcgccc tcagagagct
     2941 gggtttcagc tctatatggg aggcatcgtt cggcgctgag tccctgcttc cgtactacgc
     3001 cgactatgtg aaggcacatt cggaggaatc gggccggtcg gttgcgtttg catctatgtg
     3061 ccctgctgta gttgccttta ttgaacattg ccgtcctgag cttgctggta atctagtccc
     3121 tcttgtttct ccaactgtag ccgcgtctcg tgtggctagg gctttggggg aaaagagggt
     3181 cgtttttatt ggtccatgtc ctgcagaaaa acttgaagcc ggttttgggg gcgtgcccgg
     3241 agggccgaat attgcgatta cttatgcaga attatcgcgt ttgcttgata atgtaggagt
     3301 atctgtggat caatgccctg cctcgcttcc tgatgtaagg gcaagcggcg ctggtcgcat
     3361 gcttggattg ccggcggggt tgcttcgggc cctcagaatt agggatgatg ttgcgccgcc
     3421 cggcatcttg cttgcagaag gcaaagaaga gtgcctgaaa ctggtggaag cttcgctgtc
     3481 gggtgcatta ggcggttcat ttgtcgtagc atcgatctgc cgtagctgcg tggatgggac
     3541 tggttttcca gaaggtgctt ctttgtggga tagaaggaga gccgtggttg actacattaa
     3601 ggaagcaagt tctgtgggca gcatggaagt ggatggcgct ttgcgcgctg tgtctgcgtc
     3661 agccgatttc aagaggcgtt acttgcctag aaagattcct ctcgcgccaa aagagactga
     3721 tgtgcgcacg gcgttgctcg gcatgggaat gaggagcccg gaggacgaac tagactgcgg
     3781 gctatgcgga ttccagtcct gccgcgaaag ggcaattgcg gtggcgcgag ggacgactga
     3841 ggccagtgag tgttttccgc gcaggaatgc aaggttatct gaggaatgtg cccgtctgag
     3901 tcagcttgcg tccgaccttg attcaatcgt aaatacttcc tgcgagatct cctatgtctg
     3961 cgatccggat ggctggatag taagggcaag cagagcggct aacgacatta tcggtgtaga
     4021 gaatgcgaat ctgcacaagg ttaacatgaa gaatcttcct cgcagactat gccctgatgt
     4081 tgcgatccag gctttgcacg aggtcaagcg agtgatggcc attagggatt tgccaggcgg
     4141 gcataggttg cttgcggcgg cctcccccat acttgatggc gacggggcct gtagaggcgt
     4201 tgcggcgacg ataacggatc tgagcggggc agccttggag atcaatacaa ctcccacggc
     4261 cagcagcttc gcgactgacg ctgattccga ggacatggta gcctacagtc ctgctatgga
     4321 agagctcctc accttagttc ataaagtagc caacgtatcg tcaactgtgc ttattaccgg
     4381 agagtctggc gtgggcaaag aggttgtggc gaggtatatt cataggacgg gaaacagggc
     4441 atcggggcct tttgtcaaga taaactgcgc tgcaatacca catacgcttc ttgagtcaga
     4501 gttgttcggg tatgaggaag gggcctttac tggagcacgg aagagcggca agcctggtct
     4561 aatccaactg gcttctggag gcactctttt tctagatgag atttcagaga tgcccctggg
     4621 aatccaagcc aagcttcttc aagttctgca ggaaaaatgt cttgtccgag tgggcggtac
     4681 tgagcaaatc agggtggatg cgcggatcat ggcagccaca aatagggatc tgagggctgc
     4741 ggtgaaagag ggacggttcc gtggagatct ctacttcaga ctcaatgtca tacctatccg
     4801 ggttccgcca cttagggaga gatctggcga tatcgcgcct ctaacctatc actttattga
     4861 aaagtgggat agcagatacg ggcggaatca tcagatttca cgggaggcct gctcgctttt
     4921 ggaatgctat agatggcctg gcaacgttag ggaactcgag aatcttatag agtttcttgt
     4981 agttactgtt gatgaccccg ttatcctgcc tgaacatctt ccacagcacg ttgtggcagg
     5041 ggagggcgag agagatgtcc aggtgagcgt gtcagggatc atgcctctcc gtcaggctgt
     5101 ggaggaagtt gaacgccagc tgattgctct cgccgctgaa gcatgctcta ccacctattc
     5161 catggccaaa gctttggggg ttaaccagtc gactgtagtg agaaaaatgc agaagtacat
     5221 ggagactccc gaggaggagg cagaggagac cgggaacgtg taatgcgttt gggcattaat
     5281 cgatgcgaca cagcattaag ctttgcaaag gcaagcagag taagataata taggccgtga
     5341 gcagggatgt cgtgcggaca aacgaaggag acatgtgggc acaaatgtga aaaggatcac
     5401 gactgatgca caagcgcatt agcgagatta tactctgaaa acaaaagcgc aggtaggttg
     5461 cttaagatcc gc
//
LOCUS       DAOSAI010000141         5418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9538_length_5418_cov_3.786500, whole genome shotgun sequence.
ACCESSION   DAOSAI010000141 DAOSAI010000000
VERSION     DAOSAI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9538_length_5418_cov_3.786500"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLM83_05915"
     CDS             complement(<1..194)
                     /locus_tag="PLM83_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK70976.1"
                     /translation="MKILSTTGLKKYFPIRSGVFLQVTGYVRALESTDFEINEGETIG
                     IVGESGCGKSTLGRTLVKIY"
     gene            complement(191..1180)
                     /locus_tag="PLM83_05920"
     CDS             complement(191..1180)
                     /locus_tag="PLM83_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK70977.1"
                     /translation="MTTACERILTVSNLKTYFRTPDGIVRAVDDVSFHLNRGEVLALV
                     GESGCGKSVTAHSIMGLVKAPPAKLEGSIVFDCRELIGLSSSEYRKIRGMQISMVFQE
                     PMSSFDPLYTVGEQLMEVAQAHMPWGEEEAKERIIETLRLVHIPEPDKRYSEYPHQMS
                     GGMLQRIMIAMALITSPKIIIADEPTTALDVTIQAQVLNLMQDIQSEYDGSIIFITHD
                     LGTVAEIADRVHVMYAGKIVEKASVVELFDNPLHPYTAGLLASRVRREYKGQDLPYIE
                     GYVPRADQFPEGCRFNPRCLRATAKCRELQPPDLEPAPGHTVACWLYEGGDGR"
     gene            complement(1177..2268)
                     /locus_tag="PLM83_05925"
     CDS             complement(1177..2268)
                     /locus_tag="PLM83_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70978.1"
                     /translation="MEGTRKRIADSFFKHKLGMVGLVVLIIFYLIVIFADFVAPYNFA
                     ETHRNFIYAPPSKVKWVDSSGKWVGPHIHEMVKSRDPVTYELVYAEDPDSVVPLKFFV
                     RGEKYRFLGLWESDLHLFGVEASPEKAMFLVFGADRFGRDLFSRIVIGGRVSMTVGLL
                     GTFLSVFIGAIVGALSGYYGGWVDVLIQRFTELLRSFPRIPLWLALAVIIPPSWPSTW
                     VYFGIVTVLSLIGWMGVARVMRGMTLSLREKEYVLAAKVMGVSDWKIITKHLIPNTLS
                     YLIVVSTLSIPGMILGESTISFLGLGIKEPMTSWGLLLSQAQSLSELEAHPWLMVPGI
                     FIMIAVLSFNFVGDALRDAVDPYRTVEKV"
     gene            complement(2280..3248)
                     /locus_tag="PLM83_05930"
     CDS             complement(2280..3248)
                     /locus_tag="PLM83_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK70979.1"
                     /translation="MRAFIARRILIMIPMMLLASVICFWITELQPGDFVSQYLDNPRI
                     SPEQIRLLRERLGLDKPAHVRYFMWLKGVVTKWDFGYSFAYERPVGDLIWERMGWTVT
                     IALLTVVFQWLLAVPMGIYSALHPYSPLDYTLTVIGFIGISVPDFFLALILMYWSLKA
                     GATSIGGLFSPQFIGAPWSLAKFADLLRHLWMPLVVVGLGGLAGLMRVMRGNMLDVLG
                     SPFITSLRAYGLDEQTVKRHAVKNALNPMVSIAGSELPNIFSGTIIAAIVLNLPTMGP
                     FFYNALLNHDQYLVMAFLMFIALITQIGNLLADIALAFLDPRIRMS"
     gene            complement(3316..5085)
                     /locus_tag="PLM83_05935"
     CDS             complement(3316..5085)
                     /locus_tag="PLM83_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK70980.1"
                     /translation="MRRRLWLVVVLCLLMTTPALAAPYVEKEDLTPSTKPVRGGTLRL
                     ALPASPQSFLFYGALDNNAYTVIGQTMTGLVELHPVTNAIRPGLAESWETSPDGKEVI
                     FHLRDVKWSDGASFTADDVIFTFENFIMNPVAKGNSADRFTIVDVTDGKKKPVQWVKI
                     DNRTVKAVLPSPFGPFYMNLSHAYIYPKHKLESKIDKKRPDSVNELWLTNVNPKEIVA
                     NGPYRIASYVMDQKVVLERNPNYWKVDRFGNQLPYFDKLEYLIIKDDEVRLAKFMSGE
                     IDYMAVSAKDFPVLKGKERAGAPFTVYMAQPTQPTPSPVHISFNYDVEDRNLRTLFTN
                     IDFRRAMECLLDRDRIIDEVYNGLAIPGGGLVLPSNKAFYNPKIEEIMRPYDPRKANA
                     ALDKLGLDKRGRDGFRLFPDGKRVEFTLTVASAPQDHQDIALIFKEDLEKAGIKVNLQ
                     ILDATLVGNMFGAGNFEAGIRAFGNQPDLELRKAIWQPGTQLYYWHYSTLDKDNLAAI
                     TKNMTDWEKRVYLLFDRGSVTTDPAKRKEIYDEIQEIYYDVVPVIFVCKGMNLFAANN
                     SLGNIWQDKQGIVYFSSYTTYRK"
BASE COUNT         1205 a         1498 c         1430 g         1285 t
ORIGIN      
        1 tcgtagatct tcaccaacgt cctcccgagg gtactcttgc cgcacccgga ctcgccgacg
       61 atgccgatgg tctctccttc gtttatctcg aagtcggtcg attccagtgc ccttacatac
      121 cctgtaactt gcagaaacac ccccgacctt atgggaaagt acttcttcag ccccgttgtg
      181 gacagtatct tcatcttcca tcacccccct cataaagcca gcaggcaacg gtatggccag
      241 gcgcaggctc gagatcggga ggctgaagtt ccctgcactt ggccgttgcc cgcagacacc
      301 tcgggttgaa cctacaccct tcgggaaact ggtcggctct gggcacgtag ccttcgatgt
      361 atggcaggtc ttggcccttg tattctcgtc ggacacggga ggccaaaagc cccgctgtgt
      421 atgggtgcag ggggttgtca aagagctcca caacggacgc cttctccact atcttgcctg
      481 catacatcac atgaacccgg tccgcaatct ctgccactgt gccgaggtcg tgagttatga
      541 atattatgga accgtcgtac tcgctctgga tgtcctgcat gaggtttaaa acctgagcct
      601 gaatggtaac atccagggcc gtcgtgggtt cgtctgcgat tatgatctta gggctcgtaa
      661 tgagagccat ggctatcatg atcctctgga gcatgcctcc gctcatctgg tgggggtact
      721 cgctgtagcg cttatcgggc tcgggaatgt ggacaagcct caatgtctcg atgatcctct
      781 ccttcgcctc ttcctcaccc cacggcatgt gagcttgtgc cacctccatc aattgttcgc
      841 ctactgtgta cagggggtca aatgacgaca tgggctcctg aaagaccatg ctgatctgca
      901 tgcctcttat ctttcgatat tcgctggacg aaagcccgat cagctcgcgg cagtcaaaga
      961 cgatgcttcc ctcaagtttt gcgggaggcg cttttaccag ccccattatg gaatgggcag
     1021 tcacactctt tccacagccg gattcgccca ctaaggccag gacctcgccc cggtttaggt
     1081 ggaaactgac atcgtcgact gcgcgcacga tgccatcggg cgtgcggaaa tacgttttca
     1141 ggttcgacac agtgaggata cgttcgcatg cagttgtcat accttctcta ccgtcctata
     1201 cggatctacc gcgtccctca gcgcatcacc cacgaaattg aaggaaagca cggcaatcat
     1261 tatgaatatt ccgggcacca tcagccatgg atgggcttcg agctcgctta aggactgggc
     1321 ctggctgagc agtaatcccc aactggtcat gggctccttg atgcccagcc caaggaagct
     1381 gatggtgctt tcgcccagta tcatccctgg gatcgaaagg gtcgaaacta caatgagata
     1441 gctcaaggtg ttcggtatca ggtgcttggt gatgatcttc caatccgaga cgcccatcac
     1501 cttcgctgcg aggacatatt ccttctccct gaggctcaat gtcatccccc gcatcaccct
     1561 ggccaccccc atccagccga tgagggagag cacggtcacg attccaaagt agacccaggt
     1621 gcttggccaa ctaggaggga ttatcactgc aagcgccagc cacagtggga tcctcgggaa
     1681 agatctgaga agttcggtga accgttggat cagcacgtca acccaacccc cgtagtagcc
     1741 tgaaagcgcc ccaactatgg cgccaatgaa cacgctgagg aaggttccga gcagtcccac
     1801 ggtcatcgac accctgccgc ctatcactat cctggaaaac aggtcgcggc caaacctgtc
     1861 ggcgccaaag accagaaaca tcgccttttc aggagatgcc tctactccga agaggtgcaa
     1921 gtccgattcc cacagtccga ggaacctgta tttctccccc ctcacgaaga acttcagggg
     1981 aactacactg tcgggatcct cggcatatac cagctcatac gtgaccggat ccctgctctt
     2041 taccatttcg tgaatgtggg gccccaccca tttgccagag ctgtcaaccc acttcacctt
     2101 ggaaggaggc gcatagatga agtttctgtg cgtttcggcg aagttgtagg gggccacaaa
     2161 atcggcaaat atcacaatga ggtagaagat gatcagtaca acaaggccaa ccatacccag
     2221 tttgtgtttg aagaagctgt cagcaattct tttcctggtt ccttccatgg tggacacccc
     2281 tagctcatcc ttattctcgg atcgaggaag gcaagcgcga tgtcggcaag caaattcccg
     2341 atctgcgtaa tgagtgcgat gaacatcaga aaagccatga ccaggtattg gtcgtgattg
     2401 agcagcgcat tatagaaaaa cgggcccatc gtcggaagat tcagaacgat ggccgcaatt
     2461 attgtcccag agaatatgtt gggcagctcc gacccggcga tgctcaccat gggattgaga
     2521 gcgttcttta cagcatgcct tttgacggtc tgctcatcga ggccgtaggc cctgagcgat
     2581 gtgatgaatg gcgatcctaa gacatcgagc atgttgcctc gcatgactct catcagcccg
     2641 gccaggccgc ccaagcccac aacgaccagg ggcatccaaa gatgcctcag gaggtcggca
     2701 aatttcgcaa ggctccaagg agcgcctatg aactggggcg aaaagagccc gcctatagac
     2761 gtagcgcccg ccttgagcga ccagtacata agtattaggg cgaggaagaa gtcgggaacg
     2821 gagatcccta tgaacccgat gacggtcagg gtgtagtcaa ggggcgaata gggatgcaag
     2881 gccgagtaga tgcccatcgg cacggccaaa agccactgga agaccactgt gagcaaggct
     2941 atggtgaccg tccagcccat tcgctcccat atgaggtcgc cgaccggccg ttcgtaagcg
     3001 aaagagtaac cgaagtccca cttggtgact acgcctttca gccacatgaa atacctgaca
     3061 tgggctggct tgtcgaggcc tagtctctcc ctgagcagcc tgatctgctc cggtgatatc
     3121 ctggggttat ccagatactg actgacaaag tcgccaggct gaagctctgt gatccaaaaa
     3181 cagatcaccg acgccaggag catcatggga atcatgatca atatacgtct cgctatgaat
     3241 gcccgcattc atgccctctc ctctaaacgt gcaaatagag cagggggaag cggggcgctt
     3301 cccccttatc gaacgttact ttctgtacgt tgtgtagctt gagaagtaga ctatgccctg
     3361 cttgtcttgc catatgttgc cgagtgagtt gttggctgca aacaagttca tgcccttgca
     3421 cacaaagatc accgggacga cgtcgtagta gatctcctgg atctcgtcgt agatctcttt
     3481 cctcttggca ggatcagttg taacagaccc tctatcaaag agaagataga ctctcttctc
     3541 ccaatcagtc atgtttttgg tgatcgcggc caggttatcc ttgtcaagcg tcgagtagtg
     3601 ccagtaatag agctgagttc ccggctgcca tatcgccttc ctcagctcga ggtcgggctg
     3661 gtttccgaag gcccttattc cggcctcgaa gttgcctgct ccgaacatgt tccctacaag
     3721 cgtggcatcg aggatctgca gattgacttt gattcctgcc ttctccaggt cctccttgaa
     3781 gatcaaggcg atatcctggt gatcctgagg cgcactggcc acggtgaggg tgaactcgac
     3841 cctcttgccg tcagggaaga gcctgaaccc atcgcggcct ctcttgtcaa ggcccagctt
     3901 gtcgagggct gcattggcct tgcgtggatc gtacggcctc ataatctcct ctatcttcgg
     3961 gttgtagaag gccttgttgg aggggagcac caggccgccg ccggggattg cgaggccatt
     4021 gtaaacttca tcgattatcc tgtctctgtc gaggaggcac tccatggctc tgcggaagtc
     4081 gatgttggtg aagagcgtcc tgaggttgcg gtcctccaca tcgtagttga aggagatatg
     4141 gactgggctg ggcgtcggct gcgtgggctg ggccatgtaa acggtaaagg gtgcccctgc
     4201 ccgctctttc cccttcagta cggggaagtc tttggccgaa accgccatgt aatcgatctc
     4261 gcccgacatg aacttggcga gcctcacttc gtcatccttg atgatcagat actccagctt
     4321 atcgaagtat gggagctggt ttccgaacct gtctaccttc cagtagtttg ggttcctctc
     4381 taggacgact ttctgatcca ttacgtagct tgcgattctg tatgggccgt tggccactat
     4441 ctccttggga ttgacgtttg taagccacag ctcattgact gagtcgggcc tcttcttgtc
     4501 gatcttgctc tcaagtttgt gcttggggta gatgtaagca tgggaaagat tcatgtaaaa
     4561 cggcccgaat ggcgacggaa gaaccgcctt gactgtcctg ttgtcgatct tgacccactg
     4621 gacaggtttc ttcttcccgt cagttacatc cacgatggtg aacctgtcgg cagaattacc
     4681 cttggccacc gggttcatga tgaagttttc aaaggtaaag atgacatcat cggcggtaaa
     4741 ggaagctccg tcagaccatt tcacgtctct taggtggaag atgacttctt ttccgtcggg
     4801 agaagtttcc cacgattcgg ccagacccgg cctgatggcg tttgttacag gatgcaattc
     4861 gacaagtccg gtcatggtct gaccgatgac ggtgtaggcg ttgttgtcga gcgccccgta
     4921 gaacaggaac gattgcgggg aagccgggag cgcgagacgg agtgtgcctc cgcggactgg
     4981 ctttgttgaa ggtgtgagat cttctttctc gacataaggt gcagccagcg ccggggtggt
     5041 catcagcaag cagagaacga caaccaacca aagacgacgt ctcattgaaa gaccctcctt
     5101 atgttaaggt attagctaag aacctgggtt atccatgaca tctcagacac agcatccctt
     5161 cgcatcgcat cagtcccttc cgagcaagtt tccgcttgat aacgtctcgg tacatccccc
     5221 gcccgcgcgg gggtaactga cagcatttgc tacgcctaat cgggtcaatc cagtaatacc
     5281 aattcggcct ccatgcggga aatccttccg attaggaaaa tttatttgca ttgtatgtct
     5341 ttatcggaat aacttattct cgagctttgg cgaaggcttg gaattgatat ttgctgcatg
     5401 ctccgtgctc gccgtgga
//
LOCUS       DAOSAI010000142         5404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9571_length_5404_cov_2.759768, whole genome shotgun sequence.
ACCESSION   DAOSAI010000142 DAOSAI010000000
VERSION     DAOSAI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9571_length_5404_cov_2.759768"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2099
                     /gene="priA"
                     /locus_tag="PLM83_05940"
     CDS             <1..2099
                     /gene="priA"
                     /locus_tag="PLM83_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOK70981.1"
                     /translation="AEGAKISGAAKEVFDYLVECGGSARRDEIASSLEIADITRPISA
                     LTKAGLVETDTMWAPPRVRPRTVRAFRLASEEIDPQALALSLAPGSPARAAVMRVFAE
                     DIDKTGTKGLAIPRAELLERAGCSSSVVDALVRDGMLERMDVAIQRDPTAGLQIEKSV
                     PPVLTDDQEHAVNRITDVAENGGGVVLLHGVTGSGKTEVYLRAIQHVIDRGKQGILLV
                     PDISLTPQMVDVVMGRFGDRVALLHSALGAGERLDEWERARAGDADVVVGARSAVFAP
                     VPSLGMIIVDEEHDSSYKQDDSPRYNARDVAIFRAKQVGATVVLGSATPALETYELSE
                     LGECERIELPSRIDHRPLPEIRVVDMRREMKSGNKSVFSNLLRSSVEEALDSGQQVML
                     FLNRRGFSTFAMCLECGQAISCPNCDVSLTYHARASEMVCHYCNWSQPVPDVCPVCGG
                     MNMKYSGTGTERIEEAVRKEFPRARVARMDVETTRRKVAHRAILGAFRAGRTDILIGT
                     QMIAKGLDFPNVTLVGVISADTSLNLPDFRAAERTFQLISQVAGRSGRGSEPGLVVVQ
                     TFNPEHYSIMAAAAHDYRAFYDAEIAARREMGYPPARRMARILVRGEDESATAKAAGK
                     IRRACTGTEPVARGAVEVIGPAPAPIQRIAGKYRWHLLLFSSDADAIRAVAAAGCQPR
                     LAGVTISVDMDPSSIL"
     gene            2120..2611
                     /gene="def"
                     /locus_tag="PLM83_05945"
     CDS             2120..2611
                     /gene="def"
                     /locus_tag="PLM83_05945"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOK70982.1"
                     /translation="MLILEIRTEGDPVLRETAKPVKIIDGKMRDLARDMLETMYSAPG
                     VGLAAPQVGIPVRLIVIDAGDGPRTLFNPEIVWRRGEAVDDEGCLSVPGMIGTVTRSA
                     EVEVIAYDRDGKLVSVRGKGFLARILQHEIDHLDGVLYIDKAENVRPVEEECEESQCS
                     EGD"
     gene            2615..3598
                     /gene="fmt"
                     /locus_tag="PLM83_05950"
     CDS             2615..3598
                     /gene="fmt"
                     /locus_tag="PLM83_05950"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOK70983.1"
                     /translation="MRIVFMGTPEFAVPALRALTDAGHEIICVVTQPDRPRGRGYKLA
                     SPPVKVEAERLGLPVVQPARVSDPEFVEGFAEMAPDVAVLAAFGQKISAELLSVPRHG
                     FINIHPSLLPKYRGAAPIERAMINGDQVTGVTIMYMDEGWDTGDIGICQDMPIPSDVD
                     RGWLECALAELGGELIVKFLRLIECGEAPRVPQDHSAATYARKLSSDEFEIDWRKSAR
                     EIGNLIRALSPAPGARTSFAGQSVRILRAHEIAPVLVGDGEAGETVCSGYPGMLTIGP
                     EGELIVFCGLNGCELMALDVVHPAGKRQMPAADFARGRRISHGDRFGSGMC"
     gene            3637..4341
                     /locus_tag="PLM83_05955"
     CDS             3637..4341
                     /locus_tag="PLM83_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOK70984.1"
                     /translation="MPFYFYYDPTYIIVIPALLLAIYAQAKVQGTFNRYMRVRSKTGF
                     TGSQVAQSILRSNGIHDVGVEPINTPLGDHFDPRSKKVRLSPAVYRGSSIAALAVAAH
                     ESGHAMQDKQGYAPLAFRQAILPVANFGSHMAFPLFILGMLMGYDKTGQFLMSLGIWL
                     FAGAVLFQLVTLPVEFNASKRALTLLADGGYITQDELPAARQVLQAAALTYVAASATA
                     ITQLLRLLILRGSQRD"
     gene            4334..>5404
                     /locus_tag="PLM83_05960"
     CDS             4334..>5404
                     /locus_tag="PLM83_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOK70985.1"
                     /translation="MTRRPKFAASKTARDLAVEVAYLCDQEGMYANAAWSQALSGKEM
                     DARDRRLAMEIAYGSIRRKKTLDWWIGHASTRPVDLMDPLARALLRTGVYQLLFMSRV
                     PPHAAVSESVESAARLGHRGLASFVNAVMRKITSAEFSPEFPDPKGDPVGYLSVSLSY
                     PEWLVQSWIGEYGVDVARAMLEAGNEAPTIVLRVNRLKIEREEFSRMLKSEGVLSEPG
                     HLCPEALILSSPIDGVEELPGYHDGLFSVQDESSMLVGHVIGVAELVCAANGKKPGAL
                     WAESGKEDESGVAEEAIEDGHWPLIIDACAAPGGKAAHMAELAGCGAKVVACDVALER
                     VKMIEDSVARLGLPSVEAICCDA"
BASE COUNT         1152 a         1297 c         1739 g         1216 t
ORIGIN      
        1 atgcggaagg ggcgaagatt tcaggtgcag ccaaggaagt cttcgattac ctcgtggaat
       61 gcggcgggag cgcgcgaaga gacgagatag cttcttcatt ggagattgca gacatcacaa
      121 gacctatatc tgctcttaca aaggccggtt tggtcgagac ggacactatg tgggcgccgc
      181 ccagggttcg tcctcggact gtaagggcgt ttcgtttggc cagcgaggag atagacccgc
      241 aagctttggc gttaagtctt gctccgggca gtccagcgcg ggcagccgtt atgagggtat
      301 tcgccgagga catcgacaaa acggggacca aaggactggc gattcccagg gcagagcttc
      361 tggagagggc aggatgttcg tcatctgttg ttgatgctct cgttcgcgac gggatgctcg
      421 aacgaatgga tgtggcgatt cagcgggatc ccactgcagg ccttcaaata gagaaatcgg
      481 tgccgcccgt gctcactgac gatcaggagc atgccgtaaa caggataact gatgtcgcgg
      541 agaacggggg aggcgtggtt ttgctccatg gggtgaccgg gtcgggcaag acggaggtct
      601 atcttcgcgc gatccagcac gtgatagaca ggggaaagca aggcatcctg ctagtccctg
      661 acatatcgct caccccgcag atggttgacg ttgtgatggg gcggttcggc gacagggtcg
      721 ctctgctcca cagtgcgttg ggcgctgggg aacggcttga tgaatgggag cgggctcgtg
      781 caggagatgc agatgtggta gttggcgcca gatcagcggt gttcgcacca gtgccgtcgc
      841 tgggcatgat tattgtcgac gaggaacacg attcttcata taagcaagac gactccccgc
      901 gctataacgc cagggacgta gcgattttca gggcaaagca ggtcggcgcc actgtcgtct
      961 tgggttcggc tactccggcc cttgaaacgt atgagttgtc cgaattgggc gagtgtgaac
     1021 ggatagagtt gccatcgcgc atagatcacc gcccgcttcc agagatccgc gtcgtagaca
     1081 tgaggcggga gatgaagtcg ggaaacaagt cggtattcag caaccttctt cggagtagtg
     1141 tggaggaggc gctcgattcc ggacagcaag taatgctctt tctgaacagg cgcgggtttt
     1201 ccacttttgc gatgtgtctc gagtgcggcc aggccatttc gtgtccgaat tgcgatgttt
     1261 ccctgactta ccatgcaagg gcatcggaaa tggtttgcca ctactgcaac tggtcgcagc
     1321 ctgttcccga cgtgtgtcct gtgtgcggcg ggatgaacat gaagtattct gggactggga
     1381 ctgagcgcat agaagaggcg gtaaggaagg aatttccccg cgcgagggtt gctcggatgg
     1441 atgtcgagac gactaggcgg aaggtggcgc accgggcgat tttgggggcg tttcgggcgg
     1501 gacggaccga tattctcatc ggaacgcaga tgatcgccaa agggctcgat ttccccaatg
     1561 tgacccttgt tggggtcatc tctgcagaca ccagcctaaa ccttcccgat ttccgtgccg
     1621 cggagcgcac tttccagctc atatcccagg ttgcaggaag atcgggaagg ggatcagaac
     1681 ccgggctggt agtggtccag acattcaacc cggagcatta tagcatcatg gctgccgctg
     1741 cccacgacta ccgtgcattt tacgatgctg aaatcgctgc acgccgggaa atgggttacc
     1801 cgcctgcgcg gcgcatggcc cgcatactag tgagagggga agatgaatct gcgacagcca
     1861 aggctgcagg gaagataagg agggcgtgca caggcacgga accggtggcg aggggagctg
     1921 ttgaggttat agggccagcg ccagcaccta ttcaacgcat agcagggaaa tataggtggc
     1981 atcttcttct cttttcaagc gatgccgatg ccatccgcgc tgttgccgcc gcaggttgcc
     2041 agcctaggtt ggcaggggtt acaataagcg tagatatgga tccatcaagt attctgtgag
     2101 ctaggaatgg gaggccgtat tgttgatttt ggagatccgc accgagggag acccggtgct
     2161 tcgggaaact gcgaaaccag tcaagataat cgatggcaag atgcgtgacc ttgcccgcga
     2221 catgctcgag acgatgtata gcgctccagg agtggggctt gcggccccgc aggttgggat
     2281 tcccgttagg ttgatcgtga ttgatgcagg tgacggtccc cgcacgttgt tcaaccctga
     2341 aatcgtgtgg cgacggggcg aagcggtgga cgatgagggc tgtttgtctg tgccgggaat
     2401 gattggaacg gtcacaagat cggctgaggt agaggtcatt gcctacgaca gggacggaaa
     2461 gctcgtgtct gtacgtggaa agggcttttt ggcccgcatt ctccaacacg agatagatca
     2521 tcttgatggg gtgttgtaca tcgacaaggc agagaatgtt aggcctgtcg aagaagagtg
     2581 cgaggaatct caatgcagtg agggagactg acaggtgcga atagttttta tgggaactcc
     2641 ggagtttgct gttcctgcgc ttcgtgcgct gacagatgca ggacatgaga tcatatgcgt
     2701 tgtgacccag cctgacaggc ctcggggccg aggctacaag ttggcgtctc cgcctgtgaa
     2761 ggtggaggca gagaggttgg ggcttcctgt tgtgcagccg gcgagggtga gcgatcctga
     2821 attcgtggag ggctttgcgg agatggcacc ggacgttgcg gtgttagccg cgttcggaca
     2881 aaagatcagc gcagagcttc tttcggtgcc gcggcatgga ttcatcaata tccatccatc
     2941 gcttctaccc aaatacaggg gagctgctcc catagagcgg gcaatgatca acggtgatca
     3001 ggttaccgga gtcaccatca tgtatatgga cgaaggctgg gacactgggg acatcgggat
     3061 ttgccaggat atgcccatac cttcggatgt cgacagaggt tggcttgaat gcgcccttgc
     3121 ggagttgggc ggggaactaa ttgtgaaatt ccttcgtcta attgagtgtg gggaagcgcc
     3181 ccgggttccg caggatcatt ccgccgctac gtatgcccga aaactgtcgt ctgatgaatt
     3241 cgagatagat tggcggaaga gcgcccgcga gataggcaac ctcataaggg cattgtcgcc
     3301 cgctcccggg gctcgcacct cttttgccgg gcagtccgtg cgcatcctgc gggcgcacga
     3361 gattgccccg gttttggttg gagatggcga ggccggtgaa acggtttgct caggatatcc
     3421 gggcatgcta acaataggac ctgaaggaga gctcattgtc ttttgcggac tgaatggctg
     3481 cgaactgatg gccttggatg tggttcaccc ggctggcaaa cgccagatgc cagcagcgga
     3541 tttcgccagg ggccgcagga tatcccatgg cgacaggttc ggctctggca tgtgttgaat
     3601 gaggcatcag agtggatgcg aaaggggtga tctgggttgc cattttactt ttactatgat
     3661 ccgacctata ttattgttat accagcattg cttctggcaa tttacgctca ggccaaggtg
     3721 cagggaactt tcaaccgcta tatgcgggta aggtcgaaga cgggcttcac cggaagccag
     3781 gtcgcgcagt ccattttgag gagcaatgga atacacgatg tgggcgttga gcccatcaat
     3841 acgcctcttg gagaccattt cgatccaagg tccaaaaagg tgaggctttc tcctgcggtg
     3901 taccgcggat cgtcgatagc tgctttggct gttgcggctc acgaaagcgg ccatgctatg
     3961 caggataagc aaggctatgc ccctcttgct ttcaggcagg cgatacttcc ggtagccaat
     4021 ttcggatccc acatggcctt tcccctgttc atactgggca tgctgatggg atacgacaag
     4081 accggacagt tccttatgag cctcgggata tggctttttg ccggggcggt actctttcaa
     4141 ctggtaacgc tccctgtgga gttcaacgca agcaagcgag cgctgacgct tcttgcagac
     4201 ggaggatata ttacccaaga tgaattgccg gcggccaggc aggtgcttca agctgcggct
     4261 cttacctacg ttgcggcgtc ggcgacggct atcactcaat tgttgagatt gctcattctg
     4321 cgaggaagcc agcgtgacta gacggccaaa gtttgcggct tcgaaaactg ccagagatct
     4381 tgctgtagaa gttgcgtacc tctgtgacca ggaaggcatg tatgcgaatg ccgcctggtc
     4441 acaggcgctt tcggggaaag agatggacgc gcgtgaccgc aggcttgcca tggagatcgc
     4501 ttatggatcc ataaggagaa agaaaacttt ggattggtgg atcggacatg cgtcaaccag
     4561 gcctgtagat ctcatggatc ctctggctag ggctttgttg cgcacaggcg tttaccagct
     4621 tctcttcatg tcgagggttc cgcctcatgc tgcagtctcg gaatccgtgg agtccgcggc
     4681 aaggttaggt catcgcggcc ttgcttcttt cgtgaatgct gttatgagga agatcacgtc
     4741 tgcagagttt tctccggagt ttcccgaccc caaaggtgat cctgtaggtt atctctcggt
     4801 ctccctgtca tatcccgagt ggcttgtgca gtcatggata ggcgaatatg gcgttgatgt
     4861 ggctcgggca atgctggagg cgggaaatga agcgccgact atagtgctcc gcgtcaaccg
     4921 gctgaagatc gagcgtgaag agttttcccg catgctcaaa agtgaaggtg tgctctctga
     4981 accgggccat ttatgccccg aggcgcttat actctcaagt cccatcgatg gggtcgagga
     5041 gttgcccggg taccacgatg ggctcttttc tgtccaggac gaaagctcta tgttggtagg
     5101 gcacgttatt ggcgttgcgg agctcgtatg tgccgcgaat gggaaaaagc caggtgcgct
     5161 atgggcagaa tccggcaagg aagatgagtc gggtgtggcc gaagaagcga ttgaagacgg
     5221 ccattggccc ttgataatag acgcgtgcgc cgctcctgga ggaaaagctg cccacatggc
     5281 tgagttggcg ggatgcggtg cgaaggttgt ggcatgcgat gtagctttgg aacgagtgaa
     5341 gatgatagaa gacagcgtgg ccaggctcgg tctgccatca gtggaggcga tctgctgcga
     5401 tgca
//
LOCUS       DAOSAI010000143         5370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9658_length_5370_cov_4.049859, whole genome shotgun sequence.
ACCESSION   DAOSAI010000143 DAOSAI010000000
VERSION     DAOSAI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9658_length_5370_cov_4.049859"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3891
                     /locus_tag="PLM83_05965"
     CDS             <1..3891
                     /locus_tag="PLM83_05965"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOK70986.1"
                     /translation="THVYENSGSYKATFHAVDVNGLEATASVAVAVFDDSYFVLIDDD
                     GGNAVDEWYTNSLDRLGYKWVKLEPPVTLPTDITNPVIWFTANAARTTLTAEDQAWIG
                     EYLDNGGRLFLSSQGVIDDLDIDHPFIHEYLHVASVRKNVGAKRVFGVPGDPMTDGWD
                     ITTEPLFMDRSDSIIPDDVAVPILINDSGRACALRYVGDNGSRLIFFSFPFEFIPGGA
                     TDSGPGSGSEDRPAVKVMDAIAKFLTEPGVAQVEVSPSSLEAVMAHGTTKQETLTISN
                     KGDCDLYFDLSVAMPVQAEKNGGPDEFGYVWQDSDDPNGPAVEWVDITEKGTLAVFGD
                     DYYKELDLPFEFDFYGQKKNSILISTNGYVTFGLRGAASSHTPIPDATAPNDMIALFW
                     ADVNARDGGVYYWHDARNGRFIVSYDGVQKQNEEYEPEGSFDFQVHLYEDGRIVLQYG
                     ELDGVLDGCTVGIENSSGTVGLQVAHNEPYLHSNMAIEFTRSSDALSFKPARGVVRPG
                     ESAAIAVELDARALPEGLHKRDIVVATNDLNSPSLAVPVSIEVTPNAVPVIVRAEATP
                     SQGEPPLRVTFAAEAIDPDANGAVADMWWDFGDGSEPEHVAETSHVYAEAGRYMASFY
                     AVDDMGAEASVSLWINVARAPHAAIDVDSVDVAVLPDSQGVAYVKIQNTGDVPMRYRV
                     TGLEKPGDVKITKTAGTDELDPTSPYAISTFEVEGDSVRTLKPEAVLKSWEIPAASEG
                     GPGWPWGCGFDGEHIWVSDVKKANFKYTTDGEFTGDRFDTTQWTTGWGGDFAWDGNLM
                     WQVSVGGKNYIGGLDPQTGECLDIISDPDGIWDGHAERGLAYDPVEDVFYIGGWVDYK
                     IYKIAGKSWPEPGKILRVYDDVTLAVAGIARVGWHLVLTLNTHPDLVAVMDSITGEII
                     TWMESPSVSSIRVGGCEQDENGNVWMVYQGEQERPGMAYLLDMGLALNDPPEWMSISE
                     GEGILQPGEAAEVEVKFNASSAMKPGDEYEAQIVVTTSDPENQMLVLPVNMRINAAPA
                     ADFSFARTGEVAVRMIDESTDSDGDIAAWSWSFGDGTTSTEKNPVHEYAEIGTYEVTL
                     TVADNDGATDTITKVVDIDALPSVVVLAPFGGEVWMGTNEIRWTASDTDTLPDELLIK
                     LEFSSDGGKTWKPIMDGVENNGSYTWNTAVVEKGDRYVVRVTATDPEGGIGEGESGEF
                     TIIILSEAVVAAPNPANQNVTFYYNIAADGKLYVYDIAGRLVYTADMASAANAHDWNL
                     VATDGRPIANGLYLYVIVTEGGEVSKVGRLVVAK"
     gene            4050..4958
                     /locus_tag="PLM83_05970"
     CDS             4050..4958
                     /locus_tag="PLM83_05970"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOK70987.1"
                     /translation="MRRISRSVQRRMATVLMAALLAVAIAGQAFAAEETSDPVGTAAM
                     LNLGMGARAIGMGGAHIAVADDATAIYYNPAALDVIRDRNVTSLYARLHGAADYLALG
                     YAQESVGVGVYGLSASGIEETDEYGNVIGTFGVNDFVLAAGYGREVASGLSLGATVKV
                     YSESAPGNAGKGITGDVGVLYSMNDGKLRLGAVGRNLAGGVRYESGTTDNFERSFGAG
                     VAYLPMDNLLIAADAVFEDGFAGCAGVEYRVGKIALRAGGNYGAGGSSLTAGAGFAVA
                     NFTIDYAYQSHSVLPDSHRLSLGVRF"
BASE COUNT         1210 a         1291 c         1674 g         1195 t
ORIGIN      
        1 actcacgtgt acgagaactc aggcagctac aaggcgacat tccacgcagt ggacgtaaat
       61 gggctggagg ctactgcctc tgtagcagtg gctgttttcg acgattctta tttcgttctg
      121 atagatgacg acggcggaaa tgctgttgac gagtggtata ccaactccct tgacaggctg
      181 ggttacaagt gggtgaaact tgagccgcct gtcacgttgc cgactgacat cacaaaccct
      241 gttatctggt tcacggctaa tgcagccagg accaccctta ccgccgaaga tcaggcgtgg
      301 atcggtgagt atctggataa cggcgggagg ctgttcctct cgagccaggg tgtaatcgat
      361 gacctggaca tcgaccatcc gttcattcat gagtatctcc acgtggcgag tgttaggaag
      421 aatgtcggag ccaagcgcgt attcggtgtt cctggggatc caatgactga tgggtgggac
      481 ataacaactg agcctctgtt tatggacagg agcgactcaa ttattccaga cgatgttgct
      541 gttccgatac ttatcaacga ttcgggacgg gcatgcgctc tgagatatgt cggagataac
      601 ggttcgaggc tgatattctt ctccttcccg tttgagttca tcccgggagg agcaactgac
      661 agcgggccgg gttccggttc tgaggacagg cctgcagtga aagtgatgga tgctatcgcc
      721 aagttcctga cggagcccgg cgttgcgcag gtggaggttt caccttcctc gctggaagct
      781 gtgatggctc atggcacgac gaagcaagag acgcttacaa tatccaacaa gggcgattgc
      841 gatctctact tcgatctatc tgttgccatg cctgttcagg cagagaagaa cggaggaccc
      901 gatgaattcg gatacgtttg gcaagacagc gatgacccca atgggcctgc agtcgaatgg
      961 gtagacataa ccgaaaaagg cacgttggct gtattcggcg atgactacta caaggaactc
     1021 gatcttccgt tcgaattcga cttctacggg cagaagaaga atagcattct cataagcacc
     1081 aatggatacg tcacatttgg cttaagaggc gccgcctcca gccatacgcc cataccggat
     1141 gctactgctc cgaacgatat gatcgctctg ttctgggctg acgttaatgc cagggacggc
     1201 ggagtctact actggcacga tgctcgcaac ggccgcttca tagtctcata tgacggcgtg
     1261 cagaagcaga atgaggagta tgagcctgag ggttcgttcg acttccaagt ccatctgtac
     1321 gaggacggtc ggatcgtgct ccaatatggc gagctcgatg gagtcctcga tgggtgtact
     1381 gtgggcatcg agaattcttc tggaaccgtc ggactgcagg ttgctcacaa cgagccttac
     1441 ttgcacagca atatggctat cgagtttacg cgttcaagtg acgccttaag cttcaaacct
     1501 gcgcgtggcg tggtgaggcc tggcgaaagt gcagcgatag ctgttgaact ggatgcgaga
     1561 gcgctgccag agggattgca caagagggac attgtggttg cgaccaatga tctcaacagc
     1621 ccgtctcttg cagtgccggt ttcgatagag gtaacgccca acgcggttcc tgtgatagtc
     1681 cgcgctgaag caaccccatc gcaaggcgaa cccccgctta gagtgacgtt tgcggctgag
     1741 gctattgatc cggatgcaaa cggcgccgtg gctgacatgt ggtgggactt tggcgatgga
     1801 tccgagcctg agcatgttgc cgagacgagt cacgtatatg ctgaggccgg acgctacatg
     1861 gcatcatttt atgctgtgga cgatatgggg gcagaggcat ccgtttcact gtggatcaac
     1921 gtggctcggg cgccgcacgc tgcgatagat gtggactcgg tggatgttgc agtgctgccc
     1981 gattcccagg gtgttgctta cgtgaagatc cagaacaccg gggatgtgcc aatgaggtat
     2041 cgagtgaccg ggcttgagaa accgggagat gtgaagataa cgaagactgc cggaacggat
     2101 gaactcgatc cgacttcacc ttacgccatc tccacattcg aggtggaagg cgattcagtg
     2161 cgcacgttga agccggaggc ggttttgaag tcatgggaga ttcccgcggc cagtgaaggc
     2221 ggaccgggat ggccatgggg ttgcggcttc gacggagagc acatctgggt gtcagacgtg
     2281 aagaaagcca acttcaagta caccactgac ggggagttca caggagacag attcgatacc
     2341 acccaatgga caacagggtg gggaggcgac ttcgcctggg acggaaatct catgtggcaa
     2401 gtgagtgtcg gtggcaagaa ctacatcggg ggactcgatc ctcagacagg tgaatgcctc
     2461 gacatcatat ctgacccgga tggaatctgg gatggccatg ctgaaagagg attggcctat
     2521 gatcctgtcg aggacgtatt ctatatcggc ggctgggtgg actacaagat atacaagata
     2581 gcaggtaagt cctggcccga gcctggcaag atcctgcgag tgtatgacga tgtgacgctt
     2641 gcggttgcgg gtattgccag agtcggctgg cacctggtgc tcacgctcaa tacccatccc
     2701 gacctcgttg ctgtgatgga cagcataact ggcgagatca tcacctggat ggagtctcca
     2761 tcggtcagct cgatccgggt cggcggttgt gaacaggacg aaaacggcaa tgtatggatg
     2821 gtataccagg gtgaacagga acgtcctggt atggcatacc tgctcgacat gggccttgcc
     2881 ttgaacgatc cgcctgagtg gatgagtatt tcggaaggcg aagggattct ccagcctggc
     2941 gaggctgcag aggtcgaagt gaagttcaac gcatctagcg cgatgaagcc tggagatgag
     3001 tatgaggccc agatagtggt gacgacgagc gatcctgaaa atcagatgtt ggtcttgccg
     3061 gtcaacatgc ggataaacgc cgcgcctgcg gcggacttca gtttcgcgcg cactggcgag
     3121 gttgctgtga ggatgattga tgaatcaacc gattcggacg gcgacatagc cgcgtggagc
     3181 tggtcgttcg gggatggcac cacttccact gagaagaatc cagttcatga atacgctgag
     3241 attggcacat acgaagtgac cctgacggtc gctgacaatg atggcgcaac tgataccatc
     3301 acaaaggtag tcgacataga tgcgcttccg tcagtagtcg tgttggcgcc gtttggcggt
     3361 gaggtctgga tgggaaccaa tgagatcaga tggactgcgt cagacactga cacgctccct
     3421 gatgagctac tcatcaagct cgagttctca tcagacgggg gaaagacgtg gaagccgatt
     3481 atggacggcg tcgaaaacaa cgggtcttac acctggaata ctgctgtggt cgagaagggc
     3541 gatcgttacg tggttcgggt gactgcaacg gatcccgaag gcgggattgg tgaaggcgag
     3601 agcggagagt tcacgatcat catcctgtct gaggcggttg tggcagctcc gaacccagca
     3661 aaccagaacg tcaccttcta ctacaacatc gcggcagatg gcaagctgta tgtgtatgac
     3721 atagccggaa gacttgtcta cacggctgat atggcgtctg cagcgaatgc acatgactgg
     3781 aatctcgttg cgacagatgg acgacctata gccaacggtc tctatctcta cgtcatcgtc
     3841 acagagggcg gagaggtctc caaggtcgga aggcttgtgg ttgcaaagta gcatacagga
     3901 atatgcgggg gcgggcggtg tctgccgatc cggccccgca tcttgaagct ttatgggtcg
     3961 cgagttgcgc ctgtaggcac agcccgcggc agacgacgta gattacaaga tgaggccact
     4021 gatgtggata cgaaaggacg gagcaaatta tgcgacggat cagcaggtca gtgcagcgcc
     4081 gcatggcgac tgttcttatg gcggcgcttc ttgctgtggc cattgcggga caggcgtttg
     4141 ctgcagagga aacgagtgat ccagttggaa cagctgccat gctaaacctt ggtatggggg
     4201 cgcgagcaat tggcatgggt ggcgcccata ttgcggtagc cgacgatgca acggcgatat
     4261 actataaccc tgcggcatta gatgtcatcc gtgatcgcaa tgtgacttcg ctatacgcaa
     4321 ggctgcatgg ggcagcagac tacttggctc ttggatatgc gcaggaaagc gtgggcgtgg
     4381 gggtttacgg gctgagcgct tcgggcatcg aagagacgga tgagtatggc aacgtaatcg
     4441 gaaccttcgg cgtgaacgat ttcgtgctgg cggccggata tggtcgggag gtagcttcgg
     4501 gcctgagcct gggagcgacg gtcaaggtct actccgagtc ggcgccgggc aatgccggca
     4561 agggcataac cggggacgtt ggtgttctct actccatgaa cgatgggaag cttcgcttgg
     4621 gcgcggtagg aaggaacctt gcaggcggtg tcagatacga aagcggcacc accgataatt
     4681 tcgagaggtc ctttggcgca ggtgttgcgt acctccccat ggataacctc ctcatagccg
     4741 ctgatgccgt gttcgaggat ggctttgcgg gctgtgcggg cgttgagtat cgagtgggca
     4801 agatcgctct tcgggcaggc ggcaactacg gggccggcgg gtcttcgctt acagccggag
     4861 ccgggtttgc tgttgcgaac ttcacgatcg attatgccta tcagtctcac agcgttctgc
     4921 ctgattcgca caggctttcg ctgggcgtac ggttctagta gagctgcgac cgagtcatag
     4981 actgcacttc aggcgggctg gcgaactggg ccctgtttct gaagggcaaa gcaactataa
     5041 tgaggacccc tctctgtgtg agaggggtct ttaaataacg cattgttttc tatcgagtaa
     5101 gcgacgctga gatctatggg tcacgtcttg tcaagtggtg taaattgccg ctgtgattct
     5161 cctcgcaatt aaggttcttt agtactattg ggtaagcttg cgcttacagt ttttggtgct
     5221 gcccacgact catatgaaat tatcgagtgc cgtcgcccca cagtttcgtc cggcggtgcg
     5281 cttcaatggg catattaagc ttcagaagaa cgcacgtttt ggcgtatgcc taatcaaccg
     5341 gaggaagcgc catcgttgca tcagtcggcg
//
LOCUS       DAOSAI010000144         5367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9666_length_5367_cov_4.061559, whole genome shotgun sequence.
ACCESSION   DAOSAI010000144 DAOSAI010000000
VERSION     DAOSAI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9666_length_5367_cov_4.061559"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLM83_05975"
     CDS             complement(<1..531)
                     /locus_tag="PLM83_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HOK70988.1"
                     /translation="MSLKKTMPDRACSVTGVREIASPTDPQLGLAYVHLGMVSVEAGE
                     DRAFNFGPLETVAVILCGVAEVAWEDEDGVERRETIGGRRDVFGGPPWAFFAHPFTTF
                     EVKGLSPFVEMALVRAEQPEPGPDCLYECGCVEGGCETMEMRAGAEEIVCGRELRLDC
                     GVEIISPDEVVCRKLGE"
     gene            667..2331
                     /locus_tag="PLM83_05980"
     CDS             667..2331
                     /locus_tag="PLM83_05980"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOK70989.1"
                     /translation="MKRKRITTIIVSTLLIFVASSVWANSEDDAWRKKITEFYAYDTA
                     QPFEISFTLLNDNQWYLHYDVSYKSEGDTVYAHYFVPVTAKSQPCPCTIGLHGVFSDS
                     EEQFWMMADFLAKRGIAALAPSLPQHHRRAKGKQFVSGQRFVVGTPETIRDNLRQAVV
                     DQRRAVDWLSMRPEINPSAISIAGVSMGGIVGSLTYKIEPRIRNGVFVVAGSGVDGIV
                     DNSDLSVVSAFRQAASVKLLNPQKFIDTLQIVDPINIPDLYPRPVLMMNGTSDAIITP
                     AEALRLYNTLGKAEQIWTNGSHYYPAYAGEYLTASFLASHMPKSYGDTPPGSVLVASP
                     GYRFIEKVQPPVAGSNRLWVDLKVQIAENSKELQHKVAVPLVNRMVPVIVASRETYQR
                     IERDIINLPAFIYIMESDDASQFAAALAYIAALDLTANPLVYYAAPSQGTSCTLTQFT
                     AVQVESARHILASILSVKSSGATPVSVPKGLGSLIAQYPNHAQAGIALGKMIRYENLG
                     PLPWLPTWQDQGVRWSLGPEEPDQPKNIRREDKAGIAPWTNRSNNV"
     gene            complement(2536..3063)
                     /locus_tag="PLM83_05985"
     CDS             complement(2536..3063)
                     /locus_tag="PLM83_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HOK70990.1"
                     /translation="MKNKLTQKEQSLLSDQLNHEKLCIQKYNNYAQQAEDPQLKQLFS
                     QYASQEQEHYDTINQLLQGNQMIISTGEGRTEMIQGGPGVGQYSMEVGTIGQHASQTA
                     NHGMTGLRNQNDATLVMDMLATEKFVSSTYDTGVFESANSNVRQALQHIQQEEQQHGE
                     GLFNYLSEHGVYNPQ"
     gene            complement(3188..3346)
                     /locus_tag="PLM83_05990"
     CDS             complement(3188..3346)
                     /locus_tag="PLM83_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK70991.1"
                     /translation="MILMIGDVKIEITRKESDNLEETVRRDSVAKGAWEARRAYESAA
                     ISYIAGFR"
     gene            3674..4972
                     /locus_tag="PLM83_05995"
     CDS             3674..4972
                     /locus_tag="PLM83_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOK70992.1"
                     /translation="MAIDTPNWIKSSVIYEAFPRNHSKSGTFAGLCHDLERISRLHAD
                     ALWLMPIHPIGAANRKGSLGSPYAIRDYRAINPDLGDEQSFRELINKAHALGMKVIID
                     VVFNHTSRDSVLLAQHPEWFLQDDHGNPATKVPDWWDVYDLDYSQQGLWDYQIETLRK
                     WMQMGVDGFRCDVASLVPLDFWLTARKELSRGRKENLIWLAESVDKSFIKILRDTGWT
                     AHSDPELHAAFDLTYDYDGFDYLKGYIQGQCRLDAYLNHLYIQETLYPAHAVKMRFIE
                     NHDNPRAAEMIRDKGSLMNWTVFYTLLPGATLVYAGQEIASAHYPDLFGADPIDWANG
                     DYEFTAFMENALELSKKIKGTCTHFSVSQLVDGVVKIVWRGSGSSYVALINLAGKYGE
                     VCLDELKECHAEVCLGRREKAQEHGRCRLANTPLVVRTPA"
BASE COUNT         1301 a         1578 c         1304 g         1184 t
ORIGIN      
        1 ctcccccagc ttcctgcaga caacctcgtc aggcgagatt atctccacgc cacagtctaa
       61 cctgagctct cgtccgcaga caatctcctc agctcccgct cgcatctcca tggtttcaca
      121 accaccctca acacatccgc attcgtacag acagtcaggc ccaggctcag gctgctccgc
      181 cctcacaagc gccatctcca caaacggaga taatcccttc acttcgaacg ttgtgaacgg
      241 atgggcaaag aatgcccacg gagggccgcc gaagacatcc cttctgccgc caatcgtctc
      301 acgccgctcg acaccatcct cgtcttccca ggccacctcg gcaacaccgc acaagatgac
      361 cgctacagtt tcgagaggac cgaaattgaa ggccctgtcc tcaccagcct ccacactgac
      421 cattccgaga tgaacatagg ccagccccag ctggggatct gtaggacttg cgatctccct
      481 gaccccagtg acgctacatg ctctatcagg catcgtcttc ttaagcgaca cgtgcgcacc
      541 tcctcgtttc cattacgggc aacgccaaga tattcttcgt acagatggcc tttattcctc
      601 gtcagggcgg agttgtatta taatagaaca aacccctcaa acctaacgaa agggtagagt
      661 cctgcaatga aaagaaaacg gatcaccacg attatagtat caactttgct gatctttgtg
      721 gcaagctccg tctgggcaaa ttctgaagac gacgcctggc gcaagaagat cacagaattc
      781 tacgcgtacg atacagcgca gccctttgaa atctccttca cgttgctcaa cgacaatcaa
      841 tggtatctcc attacgatgt gtcgtacaag agcgaaggcg acacagtcta cgcccactat
      901 ttcgttccag taaccgctaa atcccagcct tgtccatgta ccatagggtt acacggggtc
      961 ttctccgatt ccgaagaaca gttttggatg atggcggact tcctggcaaa gcggggcatc
     1021 gcggccctcg cacccagtct gccacagcac cataggagag ccaagggaaa acagttcgta
     1081 tccggccaaa gattcgtagt agggactccg gaaaccatcc gggacaacct gaggcaagca
     1141 gtggtcgacc agcggcgcgc ggttgactgg ctcagcatgc gtccggagat caatccatcg
     1201 gcaatctcca tcgcgggggt cagcatgggc ggaatagtcg gttccctcac ttacaagata
     1261 gagccgagaa ttcgcaacgg cgttttcgtt gtcgctggat cgggtgtgga cggcatcgtc
     1321 gacaacagcg acctatcagt cgtctcagcc ttccgccagg cagccagtgt gaagcttttg
     1381 aatccccaga agttcatcga caccctccag atagtggatc ccataaacat ccccgacctg
     1441 tatcccaggc cagtgctgat gatgaacggg acatcggatg cgatcataac tccggctgaa
     1501 gcgcttcgtc tctataacac tttgggcaaa gcagaacaga tctggaccaa tggaagccac
     1561 tattatccgg cctatgctgg cgaatatctc acagcctcat tcctggcatc gcacatgccc
     1621 aaatcgtatg gggatactcc gcccgggtca gtccttgtag ccagcccggg ttatagattc
     1681 atcgaaaaag tccaaccccc cgttgcaggc agcaaccgcc tgtgggttga ccttaaagtt
     1741 cagattgccg aaaacagcaa ggaacttcag cacaaagtgg ctgtgcccct agtgaacagg
     1801 atggttccgg tgatcgtggc aagccgcgag acttatcagc gaatagaaag ggacataatc
     1861 aatctgccgg cattcatcta tatcatggaa tctgatgatg cctctcaatt cgcggcggct
     1921 ctcgcctata tcgccgcgct cgatctgacc gccaatccac tggtgtacta tgcagctccc
     1981 tcccaaggca ctagttgcac actgacccag ttcacagcgg ttcaggttga gtcggcaaga
     2041 cacatactcg cctcgatcct gtcggtcaag tcgagtgggg caacgcccgt gtctgtgcct
     2101 aaaggacttg gatcattaat tgctcaatat cctaaccatg ctcaagcagg cattgccctt
     2161 ggcaagatga tccgctacga gaacctagga ccgctgccgt ggctcccaac ctggcaagac
     2221 caaggcgtta ggtggtctct agggccagag gagccggacc aaccaaaaaa catacgccgt
     2281 gaggacaagg ccgggatcgc accctggaca aatagaagta acaacgtgta acctcccaca
     2341 cgcaatcaag gagagggcgc cggtcgccca accaacgccc tccccctgat tgcgcccccc
     2401 caggcctgtt acccgcaacg cgcacccgac tcagcctctc ggtcctcgct tcgtctgccc
     2461 tcctcccaaa ggcatcgttg tccttggttg cgcatggcaa cgtctgtggc cgcgttcggc
     2521 aatccgccgt tcaccttatt gcggattgta cactccgtgc tcactcaaat agttgaagag
     2581 tccttcgcca tgttgctgtt cttcttgctg gatatgttgg agagcctggc gcacgttgga
     2641 gtttgcggac tcgaagaccc ccgtatcata cgtactcgaa acgaatttct ccgtagccaa
     2701 catgtccatg accaatgtgg catcattctg gttccgcagg cccgtcattc catggttggc
     2761 agtttgactc gcatgttggc caatagtccc gacttccatt gaatactggc ccactcccgg
     2821 accgccctga atcatctctg tccggccctc gcctgtgctg ataatcatct ggttcccctg
     2881 gagcaactgg tttatggtgt catagtgctc ctgctcctgg gacgcatact ggctgaacaa
     2941 ttgcttaagc tgtggatcct cagcctgctg agcgtagttg ttgtatttct ggatgcacag
     3001 tttctcgtgg tttaactggt cactgagcaa agattgctcc ttttgcgtga gcttgttctt
     3061 caaatacacc acctcaccca ccagttttgc acaggcaaga atggtttatg cgttagaagg
     3121 cggcacaggt gcgttgatcc gagcatacag tgcggcgggc gccacgtatt ctgcagagcg
     3181 cctctcatta cctaaatccc gcaatataac tgatggccgc gctctcataa gcccgccgag
     3241 cctcccaggc gcccttagct actgaatcac gtctgaccgt ttcttccaga ttatcactct
     3301 ccttacgtgt gatctcaatc ttcacatcac cgatcatgag tatcatctct gcccctccaa
     3361 ctctgagcac cacatttatc aatcttattc atatcgttgt taatatcata aaggtactta
     3421 tgggcaatgt caatatctct tttgataatc gttagcccta acgttgctct ttcatctgcg
     3481 aacatgatat gaccttcatg caaccggcac atgccaggtg caacaaggaa atgggcctaa
     3541 ggaggattgc agtcatgcca ggcgaaatga tatgcactag ttcctcaggc aaaggccaac
     3601 gcgaaaccat gattcgcatg cctgaaggcc ctaccagctc agctgagaga ttaccacaca
     3661 ggaggtcgca tcaatggcca ttgatactcc gaattggatc aagagctcag tcatatacga
     3721 ggcatttcct cgaaaccact cgaaaagcgg aacattcgcc ggactctgtc acgacctcga
     3781 gagaatatca cgcctacatg ctgacgctct ctggctaatg cccatccatc ccataggcgc
     3841 agccaaccga aaggggagcc taggcagtcc ttatgcgatc agggactacc gcgccatcaa
     3901 ccccgacctc ggtgatgagc agtcctttcg cgaactcatc aacaaagcac atgccttggg
     3961 catgaaggta ataattgatg ttgttttcaa ccatacctcc cgcgacagcg ttctcctcgc
     4021 tcaacatcct gagtggtttc tccaggatga ccatgggaac ccggccacca aggtcccgga
     4081 ttggtgggat gtatacgatc ttgactacag ccaacagggc ctttgggatt accaaataga
     4141 gacactccgc aagtggatgc agatgggcgt ggatggtttc cgctgcgatg tagcgtcact
     4201 ggtgcccctc gatttctggc tcacggcccg caaagagctt tcgcgcgggc ggaaggagaa
     4261 cctgatatgg cttgcagaaa gcgtcgacaa gtccttcatc aagatcctgc gcgataccgg
     4321 atggacggcc cattccgacc ccgagcttca tgctgcattc gatctcacat acgactatga
     4381 cggatttgat tatctcaagg ggtacatcca aggccagtgt cgactcgacg cctacctcaa
     4441 ccatctttac atccaggaaa ccttataccc ggcacacgca gtcaaaatgc gattcatcga
     4501 gaaccacgac aacccgcgag ccgcggagat gatcagggac aagggatcct taatgaactg
     4561 gacagtcttc tatacgctgc ttcccggcgc aaccctggtt tacgccgggc aggaaatagc
     4621 ttccgcacac tatcccgacc ttttcggagc tgatcccata gactgggcaa acggcgacta
     4681 cgaattcacg gccttcatgg aaaacgcact tgagctgtcc aagaagatca aaggcacctg
     4741 cacacatttt tccgtctccc agctcgtgga cggggtagtc aagatagtct ggcgtggaag
     4801 cggttcttcc tacgtcgccc tgatcaatct ggcaggcaag tatggagaag tctgccttga
     4861 tgaactgaag gaatgccacg cagaggtgtg cttaggcaga agggaaaagg cgcaagaaca
     4921 cgggcgctgc cgactcgcga atacgccact ggttgttaga acgcctgcct gaagcgttcc
     4981 cttgaggcaa gctttgatgg agcgcctaac ggctccaaag aactgagcat caatcgggca
     5041 aggcaggcct cggcatcgta agcgatgttg acaaactact ggtcggtgca gaatcaggtc
     5101 gtgtaggatc cgtctctttt cgagtctggc agcccatact ttctctgcca aagcagcctg
     5161 cagctaggcc gaccgcacaa tcaatatcga gacagacgcg gtgtacgaaa cgcacccccc
     5221 ttcagcaaac acagctagga ggcgaaatcg atgctccgtg gcaacagagt cgttcttcgc
     5281 gaatttcgca aagaggatgc agggcgcatc caggaatgga taggtaatga gaacataacg
     5341 aagtatctgg gattcatgat gttcccg
//
LOCUS       DAOSAI010000145         5322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9754_length_5322_cov_3.992406, whole genome shotgun sequence.
ACCESSION   DAOSAI010000145 DAOSAI010000000
VERSION     DAOSAI010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9754_length_5322_cov_3.992406"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLM83_06000"
     CDS             <1..227
                     /locus_tag="PLM83_06000"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOK70993.1"
                     /translation="GTCALRVGAAACEAYLAALIAVNDTGIDDAQGIVDITIEGTAQN
                     VARLNAEGMKDVDRVLIEILEERVRRGDAL"
     gene            230..1135
                     /locus_tag="PLM83_06005"
     CDS             230..1135
                     /locus_tag="PLM83_06005"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOK70994.1"
                     /translation="MRRVYAGTSMVGGISVGTVKALAPKRLPEAGAGVVRPVGAGREG
                     ADWTAALSLPMWEYAQIWAATVATASLLTTALQRKAEDELGGAAADIFEAQHAIVSDP
                     MLEASVFSRVEEGIPLPQAVSGAITEIVAGLEASDDEATRARAEDVYDVGLRFITTLA
                     GIGAASAMDDGQFEPVLVVGGSPSEEGVLVAHDLLPSELVMLNRKMMLGLVLEAGSVH
                     SSIDVIAEALGIPVIIQAAGVLSGLVNGESIIVDADNGTVILEPYEDELKEYASRILK
                     AGHQTFYQATQAIMHDVRKPPESLS"
     gene            1274..2197
                     /locus_tag="PLM83_06010"
     CDS             1274..2197
                     /locus_tag="PLM83_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOK70995.1"
                     /translation="MIDKVTRGKIFDIRRYSVHDGPGIRTAVFFKGCPARCLWCHNPE
                     SQRREQEVMYWPNKCRGCGLCASVCDVGAVRVEHGRPVTAWELCTGCGKCAEVCPARA
                     RTIVGREVSVSEVMDEIEMDRAFYDESGGGITFTGGEPLAQPAFLCELAQTCKKVELH
                     TAVDTSGIAARHVVELVAPFVDLWLYDLKIVDRFAHLFATGCSNELAISNLRWLVKEG
                     YDVTLRIPLIPGYNDDNESLGDFIEFIGSLEWKDPPQAAILPYHRLGNEKYERLGRVC
                     CVEDLDEPDSQNIDRVRRVFEAVGIDVQIGG"
     gene            2203..4560
                     /locus_tag="PLM83_06015"
     CDS             2203..4560
                     /locus_tag="PLM83_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOK70996.1"
                     /translation="MNTRVSRLRQQSLDAVPYISAERAELITEFYANDNTPSPALKRA
                     RAFRYLMEHKAICINDGELIVGERGPAPKATPTYPELCCHSLEDLDILSSREKIPFAV
                     SPETISIYEEKIIPYWRGRSMRDLIFTEMTDEWKAAYDAGIFTEFMEQRAPGHTVADD
                     KIYRKGFRQLREDIARHLASLDYLGDPEAHDKAEQLRAMDIAAEAICIFARRHARLAE
                     SMAREETDPVRASELRRIAEICSWVPENPPRDFWEALQAYWFVHLGVITELNTWDSFN
                     PGRLDQHLYPFYKKGLEDGTLTEDSARELLQCFWIKFSNQPAPPKVGVTAAESGTYTD
                     FANINCGGLTPDGRDGVNGLTYLILDVIDEMRLLQPSSNIQLSAKNPDRFLKRALRIV
                     RKGWGQPSIFNADMVVQEMLRVGKSLEDARCGGTSGCVETGAFGKEAYILTGYFNLPK
                     ILELTLYDGVDMRTGRQLGPKTGPAALFETYEELLEAYAEQLNYFIDVKIKGNNVIER
                     LYAKYMPAPFMSLLIDDCIALGRDYNDGGARYNTSYIQGVGIGTITDSLSAIRHHVFD
                     EPRVTMDQLLQALERDYEDMEDLRRLLWDDTPRYGNDDDRADTIMTDVFKTFFDAVDG
                     RPNVKGGRYCINMLPTTCHVYFGSVTGATPDGRRAWQPLSEGISPVQGSDRRGPTAVF
                     KSASKMDHALTGGTLLNMKFSPKLLEDEAGINNLASLVRTYFKLGGHHVQFNVVKAET
                     LREAQADPEAHRDLIVRVAGYSDYFCDLSRALQDEIISRTEHQVM"
     gene            4584..>5322
                     /locus_tag="PLM83_06020"
     CDS             4584..>5322
                     /locus_tag="PLM83_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOK70997.1"
                     /translation="MLVDYHVHLERAGLSRANAMRFVDRALANGVWEIAFTEHAYNFV
                     ECAPMLERPNYVKEHGHGFRLDDYISLIEDMKAEGLSVKLGIEFDYIDETIEDVADFL
                     EEGHPWDLVIGSVHWIGEWGFDIDPTSWNDVDVEWAYSAYFERVCRAARSGVFDVIGH
                     PDVIKVFGARLATGLEGPDEEGPLRGYYDALTAAAASSGTCLEVSSAGLRRPVEEAYP
                     AMTLLKMACERGVGITLASDAHEPEEVA"
BASE COUNT         1222 a         1254 c         1628 g         1218 t
ORIGIN      
        1 aggggacgtg tgccctgagg gtcggggcgg ctgcatgtga ggcatacttg gctgcgctca
       61 tagcggtgaa cgacactggc atcgatgacg cacagggtat cgtggacatc accatcgaag
      121 ggacagcgca gaatgttgcg cggctgaatg ctgaaggaat gaaagatgtg gatcgtgtcc
      181 tgatcgagat attggaagaa cgagtccgaa gaggagatgc gttgtagcca tgagacgcgt
      241 ttatgccggg acttccatgg tggggggcat atccgtagga acggtgaaag cactggcgcc
      301 caagcgtctt cctgaagcgg gggctggcgt cgttcgcccg gttggagccg gcagggaagg
      361 ggccgattgg acagcggcat tatccctccc aatgtgggag tatgctcaga tatgggctgc
      421 aacagtggcc actgcttcgc ttctcacaac agccctgcaa cggaaggccg aggatgaact
      481 tgggggggct gctgcggata tctttgaagc gcagcacgct attgtctccg accctatgtt
      541 ggaggcctct gttttcagca gggttgagga gggcatcccg cttccgcagg cagtttccgg
      601 agctattacc gagattgtgg ccggtctgga ggcgtcggat gacgaggcaa ctcgtgcaag
      661 ggctgaagat gtttatgacg ttggcctgcg ctttatcacc actttagcgg gtattggagc
      721 agcgtctgcc atggacgacg gccaattcga gcctgtgctc gtggttggtg gctctcctag
      781 tgaagagggg gtccttgtcg ctcatgatct gctcccgtct gagctggtga tgttgaaccg
      841 caagatgatg ttgggtctcg ttcttgaggc tggatccgtg cactcaagta ttgacgtgat
      901 tgcggaagcg cttgggattc cagtgattat ccaagctgcg ggggtgctgt cggggttagt
      961 taacggggag agcatcatcg ttgatgcgga taatgggacc gtcattctcg agccctatga
     1021 ggatgagttg aaggagtatg cctctaggat actcaaagcc ggccaccaga ctttttatca
     1081 agcaacacag gcgataatgc acgacgtgcg caagcctccg gaatccttga gttaggcgtt
     1141 gggacatcag catatcttcc cagatgtctt tgagttccga catgtcagaa tgatgttgtt
     1201 aatctggaaa cgagatgcgt tttcatcttt agactccaaa aaggacatcc ctagatggcg
     1261 tggtggataa tgcatgatag acaaggtgac gcgcggcaag atcttcgaca ttagacggta
     1321 ttctgttcac gatggccctg gaataaggac cgccgtcttc ttcaagggct gtcctgccag
     1381 gtgcctttgg tgtcacaatc ccgaaagcca aaggcgtgag caagaagtga tgtattggcc
     1441 caacaaatgc aggggctgtg gactttgcgc ttctgtgtgt gatgtaggag ccgtgcgcgt
     1501 ggaacatggg cgcccggtga cggcgtggga gttgtgcact ggatgcggga agtgtgcaga
     1561 agtttgcccg gcgcgagcca ggacaatcgt aggccgtgaa gtttccgttt ccgaagtgat
     1621 ggacgagatc gaaatggaca gagccttcta tgatgagtct ggcggcggaa tcacattcac
     1681 cggcggcgag cctctggccc aaccggcatt cctatgcgaa ctggcgcaga catgcaagaa
     1741 agtagaactc cacaccgcag tcgatactag cggcattgca gcccgccacg tagtggagct
     1801 agtggcgccc tttgtagact tgtggcttta tgacttgaag atagtcgatc gcttcgcaca
     1861 cctctttgct acgggatgtt cgaacgagct cgccatctcc aacctgcgtt ggttggtgaa
     1921 agagggatat gatgttacgt tgcgtattcc ccttatcccc gggtacaatg atgataacga
     1981 gagccttggc gactttatcg aattcatagg gtccctcgaa tggaaggacc ctccccaagc
     2041 ggccatcctg ccttaccatc gtcttgggaa tgagaagtat gagaggcttg ggcgtgtatg
     2101 ctgcgttgaa gatttggatg agccggatag tcagaatata gatcgcgtcc gcagagtttt
     2161 tgaagctgtt ggaatcgatg ttcagatcgg aggctgaatc tgatgaatac gcgagtcagt
     2221 agattgcgac agcagagcct tgacgctgtt ccatatatct ccgccgagag ggctgaactg
     2281 atcacggaat tctatgccaa tgacaacact ccttcgccag cactcaaaag ggctcgtgcc
     2341 tttaggtatt taatggagca caaggccatc tgcatcaacg atggggaact catcgtcggc
     2401 gaaaggggtc ccgctccaaa agcgactccg acctatcctg aactctgttg ccattccctt
     2461 gaagacctag atattctcag tagccgggag aagataccgt tcgccgtctc gcctgagaca
     2521 atcagcatct atgaggagaa gataatcccc tattggaggg ggaggtccat gcgggatctg
     2581 atcttcaccg agatgactga tgaatggaaa gctgcgtacg atgccggcat tttcactgag
     2641 ttcatggaac agcgagctcc cgggcataca gtggcggacg acaagatcta tcgcaagggg
     2701 tttaggcaac tcagggagga tatcgcgcgg cacctggcat cccttgatta tctgggcgat
     2761 cccgaagctc atgacaaggc agagcagctc agggcgatgg acatcgctgc cgaagccatc
     2821 tgcatattcg cgcgccgcca cgctcgattg gccgaatcca tggcacggga ggagactgat
     2881 cctgtaaggg cctcagagtt gagacgcata gccgagattt gttcgtgggt gcctgaaaac
     2941 ccgccccggg acttttggga agcattgcag gcctattggt tcgtgcacct cggagtgatt
     3001 accgagctga atacgtggga ttcgtttaac cccggaaggc tggaccagca tctttacccg
     3061 ttttacaaaa aggggcttga ggacggcacg ttaaccgaag attctgcccg cgagctcttg
     3121 cagtgctttt ggatcaaatt cagcaatcag cccgctcctc ctaaggttgg tgtgactgcc
     3181 gccgagagcg gtacgtatac ggatttcgcg aacatcaact gtggaggact gactcctgac
     3241 gggcgagatg gggtcaatgg cttgacatac ctcatactag acgtgataga cgaaatgagg
     3301 ctccttcagc cgagctccaa catacagctg tcggcgaaga acccggaccg ctttcttaag
     3361 cgcgccctta gaatagtgag gaaggggtgg ggacagcctt cgatattcaa cgccgacatg
     3421 gtagtacagg agatgctcag ggtgggaaag agcttggagg acgccagatg cggcgggacg
     3481 tcggggtgtg tggagactgg cgctttcgga aaggaggcct acatcctcac cggctatttc
     3541 aaccttccta agattctgga attgacactg tatgatgggg tggacatgcg caccggaagg
     3601 cagcttgggc ccaagacagg gcctgctgcg ctgttcgaaa cgtatgagga gctattggag
     3661 gcatatgcag aacagctcaa ctacttcata gatgtaaaga ttaaagggaa caacgtgatt
     3721 gagaggcttt atgcaaagta catgccggcg cccttcatgt cactgctgat tgatgactgc
     3781 atcgccttag gtcgagatta caacgacgga ggagccagat acaatacatc ctacattcag
     3841 ggagtaggaa ttggcactat aacggattcc ctttctgcca tcaggcatca cgtgtttgat
     3901 gagccccgag tcacaatgga tcaattgctc caggccttag aacgcgatta cgaggacatg
     3961 gaggatctgc gtcggctgct ctgggacgat actcctaggt atggaaatga tgatgacagg
     4021 gctgacacga taatgactga cgtcttcaaa acgttctttg atgcagttga cgggcggccg
     4081 aatgtcaagg gtgggcggta ttgcatcaac atgcttccca ccacgtgcca tgtgtatttc
     4141 ggttcggtta caggggctac ccctgatggc cgaagggcgt ggcagcccct gtcggaaggg
     4201 atctctcccg ttcagggcag cgataggcgc gggcctactg cggtcttcaa atccgcctcc
     4261 aagatggatc atgcgctgac cggcggcact cttctcaaca tgaaattctc tccgaagctg
     4321 ctcgaagatg aagcaggcat aaacaacctc gcgagcctgg tgcgtaccta cttcaagctt
     4381 ggaggacacc atgtccagtt caatgtggta aaggcagaaa cgttgcggga ggcgcaagcg
     4441 gatccagaag cccatcgcga cttaatagtt cgtgtggcag ggtatagcga ttatttctgc
     4501 gacctttccc gcgcattgca ggatgagata atttccagga cggaacatca agtgatgtag
     4561 gcaattcgat aggggtgtgc gggatgcttg ttgattatca cgtgcatctg gaaagggcag
     4621 gattgtcgcg cgccaacgca atgcggttcg tggaccgggc gttggccaat ggcgtttggg
     4681 agatagcctt tacagagcat gcttacaatt ttgtggaatg tgcccccatg cttgagcgtc
     4741 cgaactatgt aaaagagcat ggccatggtt tcaggctcga cgactatatt tcgctcattg
     4801 aggatatgaa ggctgagggc ttgtcggtga aactcgggat cgagttcgat tacatcgatg
     4861 agaccatcga agatgtagcc gactttctgg aagagggaca tccctgggat ctggttatcg
     4921 gatcggttca ctggatcgga gagtggggat tcgatattga cccgactagc tggaacgatg
     4981 tggatgtgga atgggcgtat tccgcatatt tcgaacgtgt atgtcgtgcg gcaaggagcg
     5041 gcgttttcga cgtgataggc catcccgatg tgattaaggt gttcggcgct aggttggcaa
     5101 cgggtttgga gggcccagac gaggaaggac ccttgagagg atactacgat gctttgacag
     5161 cggcggccgc atcgtcagga acatgccttg aagtctcaag cgccggactc cggcgtccgg
     5221 tagaggaagc gtatcctgca atgacgttgc tcaagatggc ttgcgagcgg ggcgtgggga
     5281 ttaccttagc ttcggatgcg catgaacctg aagaggtcgc at
//
LOCUS       DAOSAI010000146         5321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9757_length_5321_cov_4.055070, whole genome shotgun sequence.
ACCESSION   DAOSAI010000146 DAOSAI010000000
VERSION     DAOSAI010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9757_length_5321_cov_4.055070"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /gene="radA"
                     /locus_tag="PLM83_06025"
     CDS             complement(<1..939)
                     /gene="radA"
                     /locus_tag="PLM83_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOK70998.1"
                     /translation="MKGAKVAFVCQECGYESARWMGKCPACDSWNSFVEERRPDVRVS
                     RPISSVGAGWGGSGAVAAPVPIGQISASGEKRLSTGIGEFDRVLGGGVVAGSVVLIGG
                     DPGIGKSTILLQAAQSIAASKGVVLYVTGEESVEQVGLRAARLGAGSDSLLVLAETDV
                     RAVVEAMVTIDPVLVVIDSIQTMEDVELGSSPGSIGQVRECTNRLMRAAKERGVPVFL
                     VGHVTKSGAIAGPKALEHVVDTVLYFEGEGNHAHRIIRAAKNRYGSTNEVGIFVMTDR
                     GLAEVENPSEALLSERPGGAAGSVVVASGEGTRPMLV"
     gene            complement(979..3444)
                     /locus_tag="PLM83_06030"
     CDS             complement(979..3444)
                     /locus_tag="PLM83_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOK70999.1"
                     /translation="MFGSFTERAQRVLYLSQEEARKLGHSFVGTEHILLALTCESQGV
                     AGRALHNMGVEPEAVRDEVESIIGRGEPGQAEMVGLSPRAKRVLELSIDEARLMGHGY
                     VGTEHLLLGLLREGEGVAAQVLENLGVDLDRARAEILDLLGGGVSGSSQRQEAQGQAG
                     AKLQRRKGTPTLEQYGRDLTAMANEGKLDPVIGRETEISRVIQVLSRRTKNNPVLIGD
                     PGVGKTAIVEGLALKLNRGDVPELLKEKRVIQLDMGSLVAGTKYRGEFEERLKKVIDE
                     IIASGDVILFIDELHTVVGAGAAEGAIDAANILKPALARGEMQCVGATTIDEYRKYIE
                     KDAALERRFQPIQVGEPSVEETVEILDGLRDRYEAHHRVKITDGALRAAAVLSDRYIT
                     DRFLPDKAIDLVDEAGSMVRLAAHVAPPDIKRLEDDLERVRTEKEAAIQNEEFEKAAE
                     LRDHEQSIRDTIEEQRKQWQNQRYLEGAVVTENQIAEIVASWTGIPVMQLEESEAERL
                     LKLEDILHKRVISQDEAVTAVARAVRRAHAGLKDPRRPVGSFLFLGPTGVGKTELARA
                     LAEALFGDEDAMIRLDMSEYMERHTVSRLIGAPPGYVGYEEAGQLTEKVRRRPYSVVL
                     FDEIEKAHPEVFNILLQVLEDGRLTDGQGRTVDFRNTVLIMTSNVGATQIHRDTVLGF
                     RTEEDEKNSYERMRDKVLSELKRTFRPEFLNRVDEVIVFHALGRKDMEEIVDLMLGDL
                     KAHLEPRGIHLTFTDGAKSLLVEEGYNPDYGARPLRRVIQRMVEDRLSDEILEGNIRD
                     GQSVEVNEAEGKLVFKALELQRE"
     gene            complement(3445..4512)
                     /locus_tag="PLM83_06035"
     CDS             complement(3445..4512)
                     /locus_tag="PLM83_06035"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007203581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOK71000.1"
                     /translation="MAAKRPYEKNRAVWMAGDGPDADIVVSSRVRLARNLRGVPFPQQ
                     ANDEQMKSVLEQVMGAVRATPSIGPTEILALQELLPIERQLAVEEHLTSPQHIQDPKN
                     KAIVLNRDKSVSIMVNEEDHMRIQTILPGFQLEEALRLASVTDDAVEETIDYAFDENL
                     GYLCACPTNVGTGLRASVMVHLPALALVGMVGQVLGAVSKVGVAVRGAYGEGTEAIGN
                     MFQISNQVTMGRAEEEIVAHLKAVCGQLADSERNARKAIMSDARNQLEDRVWRSYGLL
                     TNARIISSDEALKLISDVKLGADLGIIPDLPQSCFTVLSVDIMPAHVQDFVGKELTAW
                     ERDMCRATLIRERLGMNKEEH"
     gene            complement(4490..5086)
                     /locus_tag="PLM83_06040"
     CDS             complement(4490..5086)
                     /locus_tag="PLM83_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOK71001.1"
                     /translation="MICQVCREKPATVHVTKVVNNVRTELHLCQQCARERGELHVLST
                     PSFAISNLLAGLLQGDLGHEVHVGIPDGAGRKCRTCGRDYSEFVNSGFLGCADCYSEF
                     APLLNPLLGRIHGHVRHTGKAPRRAGKAVRFRREIDDLRRRLQQYVEAEEYEKAAIVR
                     DEIRRLEKAAAEVEIDGTGGDAEAKEKGGSGDGGEETV"
     gene            complement(5104..>5321)
                     /locus_tag="PLM83_06045"
     CDS             complement(5104..>5321)
                     /locus_tag="PLM83_06045"
                     /inference="COORDINATES: protein motif:HMM:NF036474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71002.1"
                     /translation="ESVGDAIDRKAAEDLIARLVDMGALDCKDAACVRAAVFDQESDE
                     DPELEGIIRARVLRAVLMLMLTRPPME"
BASE COUNT         1073 a         1776 c         1218 g         1254 t
ORIGIN      
        1 gacgagcatc ggacgtgtcc cttcaccgct ggcaacgacg acagagccgg ccgcaccccc
       61 tggccgctcc gacaacagtg cctcagaggg attctcgacc tccgctagcc cccggtctgt
      121 cataacaaat atccccactt cattggtgga accatatctg ttcttggcag ctcgaattat
      181 cctgtgagca tggttgcctt caccttcaaa atacaagacc gtatcgacaa cgtgttcgag
      241 cgccttagga cctgcaatgg ctccggattt ggtcacatgg cccactagaa atacagggac
      301 ccctctttcc ttggcagccc gcatcagccg gttcgtgcat tcgcgcacct gccctatgct
      361 tccgggagac gagccaagct ccacatcttc catggtctga atcgaatcta tcacaacgag
      421 aaccgggtcg atcgtgacca ttgcttccac taccgcgcgc acatcagtct ccgccagcac
      481 caaaagcgaa tctgacccgg caccgagcct tgcggctcta aggccaacct gctcaactga
      541 ttcttcgccc gtcacataaa gaacgacacc tttggacgca gctatgctct gggccgcctg
      601 aagcagtatg gtggacttgc cgatcccggg gtctccgccg ataagcacca ccgaaccagc
      661 taccacaccc ccgccgagca cgcggtcgaa ttcgccaatc cctgtggaaa ggcgcttttc
      721 gccgctagca gatatctgtc ctataggaac cggcgcggca accgcgcccg atcctcccca
      781 cccggcccct acgctcgata tgggccgcga taccctaaca tcgggtcggc gctcctcgac
      841 gaaactgttc cagctgtcac acgccgggca cttgcccatc catctcgcag actcatagcc
      901 gcattcctgg catacgaacg ccactttcgc gcccttcata cagactcctc tttctcaaaa
      961 catcgaggct cgcgaggttc actcccgctg tagctccaat gccttaaaga cgagcttccc
     1021 ttcggcctcg ttcacctcta cgctctgccc atcccttatg tttccttcta ggatctcatc
     1081 tgacagtcgg tcctccacca tcctctgaat cactcgtctg agcggccttg cgccatagtc
     1141 tggattatag ccctcttcaa ccaggagcga ctttgcccca tcagtgaaag tcaggtgtat
     1201 gccccttggc tccagatggg ctttgaggtc tcccaacatc agatcgacga tctcctccat
     1261 gtccttgcgc cccaacgcgt ggaagactat gacctcatca acgcggttga gaaactcagg
     1321 cctgaacgtg cgtttcagct cggaaagaac cttgtcgcgc attcgttcat aggaattctt
     1381 ctcgtcctct tccgttctga atcccaacac cgtatcgcgg tgtatctggg tcgctcctac
     1441 atttgacgtc attatcagca cagtgttcct gaaatcgact gtcctgccct gtccatcggt
     1501 gagccttccg tcttcgagca cctgaagaag gatgttgaag acttccgggt gggccttttc
     1561 tatctcgtca aacagcacca ctgaatacgg acgcctccgc actttctcag tgagctgccc
     1621 cgcctcttca tacccgacat atcccggagg ggcgcctatg agccgcgaca cagtatgcct
     1681 ttccatatac tcagacatat caaggcgtat catggcatct tcgtctccaa atagcgcctc
     1741 agccaacgcc cgggccagtt cggtttttcc gacacctgta ggccccaaaa agaggaaaga
     1801 ccctacggga cgccgcggat ccttcagccc tgcatgggca cgcctgactg ccctggccac
     1861 cgcggtgact gcttcatctt gagatatcac ccgcttatgg aggatatcct ccagcttcag
     1921 caggcgctcg gcctcgctct cctcgagctg cattactgga atgcctgtcc atgaagccac
     1981 gatctctgca atctggtttt ccgtaaccac ggcaccctcc aggtatcttt gattctgcca
     2041 ctgcttgcgc tgctcctcta tggtgtctcg gatactctgc tcatggtcac gcagctcggc
     2101 cgctttctcg aattcctcgt tctgtatggc tgcctccttc tccgtcctga ctcgctcgag
     2161 gtcatcttcg agcctcttga tgtcgggagg agctacatgc gctgccagac gaaccatcga
     2221 tccagcctcg tccaccagat ctatagcttt gtcgggcaga aaccggtcgg tgatgtacct
     2281 gtcagacaag accgccgccg ccctcaaagc cccgtctgtt atcttcaccc tgtggtgagc
     2341 ctcataccta tcacgaaggc cgtcgaggat ttcaactgtc tcctccacac tgggctcgcc
     2401 cacctggatc ggctgaaatc tccgctcgag cgcagcatct ttttcgatgt attttctgta
     2461 ctcatcaatc gttgtcgccc caacgcactg catctcgcca cgggcaagcg caggctttag
     2521 aatgttggcg gcgtctatag caccctcagc ggcacccgct cctaccaccg tgtgcagctc
     2581 atctatgaaa agaatgacgt cgcccgaggc aattatctcg tctattacct tcttgagcct
     2641 ttcctcaaac tctccacggt acttagttcc ggcaaccaaa gaacccatgt cgagctggat
     2701 cactctcttc tctttgagga gttccggaac atcgccccgg ttcagtttga gcgcaagccc
     2761 ttcaactatg gctgtcttgc ctacccctgg atcccctatg aggaccggat tattcttcgt
     2821 tctgcgagaa aggacctgaa taaccctgga aatctcggtt tcacgcccga tgacggggtc
     2881 aagcttgcct tcgtttgcca ttgcagtcag gtcgcgccca tactgctcga gcgtaggtgt
     2941 ccctttcctt ctctgaagct tggcccctgc ctgcccctga gcttcctgcc gttgggacga
     3001 tccagagact cccccaccaa gcaagtcgag aatctctgct ctggcacggt ccaaatcgac
     3061 cccgaggttc tccaagacct gtgcagcgac accttcgccc tccctgagga ggccgagtag
     3121 aaggtgctca gttcctacat aaccgtggcc catcagcctg gcttcatcta tggaaagctc
     3181 caggacgcgc tttgccctcg ggctgagccc aaccatctcc gcctggccag gctcgccgcg
     3241 gcctataatg ctctcaactt cgtctcgcac cgcttccggc tctaccccca tgttatgcag
     3301 agcgcgcccg gccacgccct ggctttcaca agtcaacgcc aggagaatgt gctcggtgcc
     3361 cacaaagctg tggccgagtt tgcgagcttc ctcttgtgac aggtagagca ctctttgcgc
     3421 tctctctgta aaagatccga acatctaatg ttcctccttg ttcatcccta aacgctctct
     3481 gataagtgtt gcccgacaca tatctctttc ccaagcagtg agctctttgc ccacgaagtc
     3541 ctgcacatgc gcgggcatga tatcgactga taacaccgta aaacaggatt gcggaaggtc
     3601 cgggatgatc ccgagatcag ccccgagctt cacgtcagag atgagcttaa gtgcttcatc
     3661 tgagctgatg atcctggcat tggtcagcaa tccataggat ctccagaccc tgtcctccag
     3721 ttgattcctg gcgtccgaca ttatagcctt tctggcgttg cgctcgctgt cagcaagctg
     3781 cccgcataca gccttgaggt gcgcaactat ctcctcttcc gctcgaccca tcgtaacctg
     3841 gttgctaatc tggaacatgt tcccgatagc ttcggtgcct tcaccgtagg cgcctctcac
     3901 cgcaaccccg accttagata ccgctcccaa tacctgcccc accatgccca ccaaagcaag
     3961 ggcaggcaaa tgcaccatta ccgaagcccg taaccctgtc ccgacattag tgggacaagc
     4021 acacaaatat cccagattct cgtcgaacgc gtaatcgatc gtctcctcca ccgcgtcgtc
     4081 agtaaccgaa gctaacctga gagcctcttc aagctggaac ccgggcaata tcgtctgtat
     4141 cctcatgtgg tcttcttcgt tcaccattat gctaaccgat ttgtcccgat tgaggacgat
     4201 cgccttgttt tttgggtcct gtatatgttg cggcgaagtc aaatgctcct ccaccgcaag
     4261 ctgtctttct ataggaagca gttcctgtaa ggccagtatc tcagtgggcc caatggacgg
     4321 agttgccctg acagccccca ttacctgctc aagaacggat ttcatctgct catcattggc
     4381 ctgctgtgga aagggtacgc cgcgcagatt ccgcgcgagc ctcacccggc tagataccac
     4441 gatatccgca tctggaccat ccccggccat ccatactgcg cgattcttct catacggtct
     4501 cttcgccgcc atccccactg cctccctttt ccttagcttc cgcgtcgccg cctgtgccat
     4561 cgatctcgac ctcagccgca gccttctcta gcctgcgaat ctcgtcgcgg actatggccg
     4621 ccttctcgta ttcttccgcc tcaacgtact gctgcagcct cctgcggagg tcatctattt
     4681 ctctgcgaaa acgcacagcc ttccctgctc ttctgggcgc ctttcctgtg tggcgtacat
     4741 gtccgtgaat gcgtcccaaa agagggttaa gcagcggcgc aaactcagaa tagcaatccg
     4801 cacacccaag aaacccgcta ttcacgaact cagaataatc gcgcccacac gttctgcact
     4861 ttctgccggc gccatcggga atcccaacat gcacctcgtg gcccaggtca ccctgaagaa
     4921 gcccggcgag taggttggaa atagcaaaac tgggcgtgct cagcacgtgc agttccccac
     4981 gctccctggc acattgctgg cagaggtgga gttcagtgcg gacattattc acgacctttg
     5041 ttacatgcac agtcgctggt ttctcacgac acacctggca aatcattgct taccctccct
     5101 gttctactcc atcggcggcc gtgtcagcat cagcataagc acggcacgga gcacccgcgc
     5161 ccgtatgatc ccctcaagtt cagggtcttc atcgctttcc tgatcgaaga ctgcggctcg
     5221 aacacaggcc gcgtctttgc aatcgagggc gcccatatca acaagcctag cgatcaggtc
     5281 ttccgctgcc ttacggtcaa tagcatctcc tacggactcg t
//
LOCUS       DAOSAI010000147         5319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9761_length_5319_cov_3.943579, whole genome shotgun sequence.
ACCESSION   DAOSAI010000147 DAOSAI010000000
VERSION     DAOSAI010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9761_length_5319_cov_3.943579"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..102)
                     /locus_tag="PLM83_06050"
     tRNA            complement(27..102)
                     /locus_tag="PLM83_06050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67..69),aa:Arg,seq:acg)
     gene            complement(253..4491)
                     /locus_tag="PLM83_06055"
     CDS             complement(253..4491)
                     /locus_tag="PLM83_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71003.1"
                     /translation="MTEMRAFCCRRRGPSACRAFSLTIFVLASAVLISFMASEAPAYA
                     AEKQEAPLQIGGDVEFKLRSAWGDSAALLGTGYTPGRFDLMQLLSLNVRGQVAPGLTI
                     SANLDNRKDGNLQVMELKLDGDPLKGRFGGLSFRSQTPYTSYSARLRGLELNAKFPEV
                     EMGVTFGRVQGIAAQRTFQGNTAQEVIVYEPAATYGPSPSASGFSASIDGMEYYLLSS
                     SYDPDFMGCWIRYDDEDELGEGRTLEETLNLWDLGYLYLEDPEARGGISAGGKVPLAR
                     GQFITVSSSEEMLALRSEIRDLLRGQIQSMIRSYNSEHGLVGSAQKRYPFVYGSETET
                     AFLDDLISRHAHIIAGMEDGGPGTTVLDARADSYLRRRLYDLGQTDVVPGSVAVEVRK
                     AGKFLSVETELPLIYQVAYDIGVIEFDFPPSFFDIYDAVRIKYRHTVAMGIFNLGLSI
                     AQGSEKVYLNDVLLQKNIDYTIDYELGILTILRTLGPDDVVKVEYEYFRGPFGAVADY
                     KSNFYGAIFGWTPNENLKLKMDVAVYSDDPKSAAMPEATSTMPNTHAIIGFSGQYDNK
                     GLNLSTDLAFSHDQFPFDDNRKTNAANRISGIMGAVDAESKSYLLISHSDGVSVGGDE
                     FRNYSIGSGLASPVVRDVASADENWFFATDGGLTVLSATPGPAGQNPFDYVSNWRRIY
                     MSAGLPSNNLTCVATTPWSVWVGTADKGVACADLADLDTWTVYRKSAGSGLPSDAISD
                     IAYDPAHDLILVGFGDGVAVLEGAHFEHELAGVGVTSIVSATSAEAVDGWRTFAAAKD
                     GVYARDEAGGWTKVVDDSIVKDPAAIEVWNRLLWIGGRNGLWVWDGRNCDIVEATRGY
                     SITALGVGRGRKYEGGETLWAGTAGIPAGTGKDPYRIVVFEVLTPEIVAEHDGTELGV
                     SGHDPRRYVEIAAQDHTTDGYAARATARYSLGSASIYGNYETLAPGFTKLGQTSRQAM
                     DVWRLGTQWPVGSKANVTAEHSESRTQSHSEKGEQPIGREVLVISNRVGGTIDIGPKI
                     DVSYVLSQVDDNEADGFEREEHTLSITGRQSFFENKLTLGAGYETVQSENLIRPGSSY
                     LQVNLRGDATFKIDALSVSALYRKPVKTVAPGGADQRVTGTEEVSLNAQWGKQLGPIA
                     LKAYYRQTLRDDIATERRFDDKRAEVRATLPVLSFNENALTPSTVLKWEKTVPYSGQA
                     KEGITVQGSLSGNLGSFRTSGGINLKRTEYPGIEKLTLDTEVFATLSSTTRGRFVPQF
                     DLRWKRSSSERAGLGVVASDSVTGTARAIYTPKQGLSNVASLAYSLTSSSGSMGVQKH
                     SVSLQDTIDYRLNEKVTITGEASVRLTAPELGALAGGEGSDIRGALKSGLRYRISDMW
                     SFGASAGYNVYKAPASQNGFAKGLTLEAGLRAVF"
     gene            complement(4642..>5319)
                     /locus_tag="PLM83_06060"
     CDS             complement(4642..>5319)
                     /locus_tag="PLM83_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71004.1"
                     /translation="ANVEGHKVTSLYSTLYGAGNYIGAGYAQKNVGVGVIGLMSTVTE
                     KDEYGNPGNEFGYREGALMGGYARNFGMFSFGGSLKLYSQTIPNNEGFGVTGDVGALI
                     EMPWMEGFRLGAVARNVGGTAKWQSGYTDQFDPEYVIGASMKPIPKLVVAADFDISNA
                     TGHVGAEYKIMPQFAVRAGGVFAEDGDMRFTAGAGFSVQNFQIDYAYQGHTALPDSHW
                     LSLGMSF"
BASE COUNT         1196 a         1665 c         1269 g         1189 t
ORIGIN      
        1 gggccacaca aacatgctcc gaaaaatggc gcgcccggcg cgattcgaac gcgcgacctg
       61 cggattcgta gtccgtcact ctatccactg agctacgggc gcactcaacg ggcctgccaa
      121 atagcaaggc cacatcgctc acacattata tcacgcgcat cccgcgcaag tcaacgatac
      181 ccttctccaa tttacagaag ctgccatgca tattctccag gcaatcagcc gcatagttgg
      241 cgtcctctct ccctagaata ctgccctcag ccccgcttcc aaagtcagcc cttttgcaaa
      301 cccgttttga ctggccggag ccttgtatac attatagccc gctgaggcgc cgaacgacca
      361 catgtcactg attctgtaac gaagcccact cttgagcgct cccctgatat cagaaccctc
      421 gcctccagca agagccccaa gttcgggggc ggtcaacctc acagaagcct cccctgttat
      481 cgtaaccttc tcgttcaacc tgtaatcgat agtatcctga aggctcacag aatgcttctg
      541 cactcccatc gatcctgatg atgatgtgag ggagtatgcc agcgatgcta cgttgctcag
      601 tccctgtttg ggagtgtaga ttgcccgagc tgttcctgtc accgaatcgc tcgctacaac
      661 ccccaacccc gccctctcag aactcgatcg tttccacctc agatcgaact ggggaacaaa
      721 ccgcccgcgc gttgtcgaac tgagggtagc gaagacctca gtatccaaag tcagcttttc
      781 gatgccggga tactcagttc gcttcaagtt aatcccgcct gatgtccgga aggagcccag
      841 attacccgac aggcttccct gaacagtgat gccctccttg gcctggccgg aatagggcac
      901 agtcttctcc cacttcaaca ccgtgcttgg agtcagagca ttctcattga acgaaagcac
      961 tggcaatgtc gcgcgaactt cagcgcgttt atcgtcgaac cttcgttctg tggcaatatc
     1021 gtcgcgcaaa gtctgtctgt agtatgcctt gagcgctatt ggaccgagct gtttccccca
     1081 ctgcgcgttc aggctgacct cttcagtgcc tgttacccgt tggtccgccc ctcctggcgc
     1141 cacagttttc acaggttttc gatacagcgc cgacacagaa agagcatcga ttttgaacgt
     1201 cgcatcgccc cgcagattta cctgaagata tgagctgcct ggcctgatca aattctcact
     1261 ttggaccgtc tcgtacccag cacccaatgt cagtttgttt tcgaagaagc tctgccgccc
     1321 cgtgatagac agagtgtgct cctcccgttc gaacccgtcg gcttcgttgt cgtccacctg
     1381 agacagaaca tatgagacat ctatcttcgg accgatatct atggttcctc cgactctatt
     1441 cgatataacg agcacttccc tgcctatcgg ttgctcgccc ttctctgaat gcgattgggt
     1501 tcgcgactcg gaatgttcag ccgtaacgtt ggccttcgaa cctacaggcc attgtgtccc
     1561 caaacgccag acgtccatgg cctgcctcga tgtctgccca agtttggtga atcctggtgc
     1621 caaggtctcg tagttcccgt agatgctcgc gcttcccagg gaatatcttg ccgttgctcg
     1681 ggcggcatac ccatcagtgg tgtgatcttg agcggcaatc tcaacatacc gtctcggatc
     1741 atggcctgat acacctaatt cagtgccgtc atgctcagca acaatctcag gcgttagcac
     1801 ttcgaacacg actattctgt agggatcctt ccccgttccc gccggtattc ccgcagttcc
     1861 ggcccagagc gtctcgccgc cttcgtattt gcggccacga ccgacgccta aagctgtgat
     1921 ggaataccct cgggtggcct ccactatatc gcaatttctc ccgtcccata cccacaaacc
     1981 gtttctgccg cctatccaca gcagcctgtt ccaaacctca atggcagcag gatccttcac
     2041 tatcgaatca tctacgacct tggtccatcc accagcctcg tccctagcat acacaccgtc
     2101 tttggcggct gcgaaagtgc gccaaccgtc caccgcttcc gcactggtcg ccgacacgat
     2161 tgacgtcacg cccaccccgg caagctcgtg ttcaaaatgc gcgccctcca acacggccac
     2221 gccgtcaccg aaccccacca ggatgagatc gtgggccgga tcataagcaa tatcggaaat
     2281 ggcgtccgaa ggcaaccccg acccagcgga cttcctgtac accgtccacg tgtcaagatc
     2341 ggccaaatct gcacaggcca cacccttgtc agcagtgcca acccaaacag accatggagt
     2401 tgtagccaca caagtcaagt tgttgctggg caagcctgca gacatgtaga tccttcgcca
     2461 gttactgacg tagtcgaatg gattctgccc cgcaggcccg ggggttgcag agaggaccgt
     2521 aagtcccccg tctgttgcaa aaaaccagtt ttcgtcagca ctggccacgt ctcgaactac
     2581 aggggatgca agacccgagc ctatgctgta gttgcggaac tcatctccgc caacgctcac
     2641 cccatcgctg tgggagatca acaagtatga tttggactcc gcgtcaactg cccccatgat
     2701 tccggatatt ctgttcgccg catttgtctt ccggttgtcg tcaaacggaa actggtcatg
     2761 gctgaaagcc agatctgtgg agaggttgag ccccttattg tcgtattgcc cggaaaagcc
     2821 tatgatcgca tgcgtattgg gcattgtaga ggtcgcttcc ggcatagcgg ccgattttgg
     2881 atcatcagag tacacggcaa catccatctt caacttgaga ttctcattcg gcgtccagcc
     2941 aaatatcgca ccataaaagt tggacttgta atcagctact gcgccaaacg gacccctgaa
     3001 atactcgtac tccactttaa ccacatcatc cggcccgagc gtcctcaata tggtgagtat
     3061 cccgagctcg tagtcgatcg tgtagtcaat gttcttctga agaagcacat cgttgaggta
     3121 taccttttcg cttccttgag caatggagag gccgagattg aagatgccca tagccacagt
     3181 gtggcggtac ttgatacgca ccgcgtcata aatatcgaaa aagcttggcg gaaaatcaaa
     3241 ctcgatcaca ccgatatcgt aagccacctg gtagatcagg ggcaattctg tctccacaga
     3301 cagaaacttg ccggccttgc gcacttccac cgcaaccgat ccaggaacaa cgtcggtctg
     3361 ccccagatca tagagacgcc tgcgaagata ggagtcggcc cttgcatcta agacggttgt
     3421 gccaggtccc ccgtcctcca tgcccgcaat gatgtgggcg tggcgcgaaa tcaggtcgtc
     3481 aaggaaagcc gtctcggtct cactcccata gacgaacgga tatctcttct gagcgctgcc
     3541 tactagcccg tgctcggaat tgtaagaccg tatcatcgac tgaatctgac ctctcagaag
     3601 atctcggatc tctgaccgca aagctaacat ctcttccgac gaagaaaccg tgatgaattg
     3661 tccgcgcgca agtggcacct tgccaccggc agatatccct ccacgtgctt ccggatcctc
     3721 aagatacaag taacccaagt cccacaggtt caaggtctct tccagagtcc tgccttcccc
     3781 gagttcgtct tcgtcatcat accgaatcca gcatcccatg aaatccgggt catatgagct
     3841 tgacaacaga tagtattcca tgccgtcaat ggaagccgaa aacccgctgg cagacggcga
     3901 cggtccatac gtggccgcgg gctcgtagac tatcacttcc tgagcagtat tgccctggaa
     3961 tgtcctctgt gccgcaattc cctgcacccg cccgaacgtg acacccattt ccacctctgg
     4021 aaacttcgcg ttcaattcaa ggccgcgcaa acgagcggaa tacgaggtat atggggtctg
     4081 actcctaaaa gacaggccgc cgaaacgacc ctttagcggg tctccgtcaa gcttcagctc
     4141 catcacctgg aggttgccat cttttcgatt gtccaggttg gcggagattg tcagccctgg
     4201 cgccacctga ccccgcacgt tgagtgagag caactgcatc aggtcgaagc gcccgggtgt
     4261 ataaccagtt ccaagaagag cagccgaatc gccccaagcg gacctcagtt tgaactcaac
     4321 atcgcccccg atctgaaggg gcgcttcctg tttctccgca gcatatgcag gtgcttcgct
     4381 cgccataaac gagatcaaga ctgccgacgc aagcacaaaa atggttagcg agaaagcgcg
     4441 gcacgcactc ggcccgcgtc ttctgcagca gaaggctctc atctccgtca cctctgacgc
     4501 cacccgcaaa tacagactgc cggatcaact ccgcagttca tctgcgggga aggcaatgtg
     4561 ggctttctag ccccaaatcc ctaattcaac aaaaacaggg ggtcccctgc acggttgtga
     4621 gaggaccccc gcctagatca cctagaacga catgcccaac gaaagccagt gtgaatcggg
     4681 cagcgccgta tgaccctggt aggcgtagtc gatttggaag ttctggactg agaagccggc
     4741 cccggccgtg aatctcatgt cgccgtcttc agcgaaaacg cctcctgccc tcactgcaaa
     4801 ttgaggcatg atcttatact cagcgcccac atgcccagtt gcattgctga tgtcgaaatc
     4861 cgcagctacc acaagcttcg gaatgggctt catagaagcg ccgatgacat attccggatc
     4921 gaactggtca gtatagccgc tctgccattt ggcggttcca cccacgtttc gcgcaaccgc
     4981 tcccagtctg aacccttcca tccatggcat ttcgatgagg gcaccgacgt ctccggtgac
     5041 gccaaaccct tcattgttgg ggattgtctg ggagtatagc ttgagtgatc cgccaaaaga
     5101 gaacatgccg aagttccgag catatccgcc cattaaggcg ccctcccggt agccgaattc
     5161 gttgccggga ttgccgtact cgtccttctc ggtcaccgtc gacatcaggc cgatcacgcc
     5221 cactcccacg ttcttctggg catatcccgc gccaatgtag ttgccggcac catagagggt
     5281 cgaatacaag gatgtgacct tgtgtccttc cacattggc
//
LOCUS       DAOSAI010000148         5298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9818_length_5298_cov_3.656494, whole genome shotgun sequence.
ACCESSION   DAOSAI010000148 DAOSAI010000000
VERSION     DAOSAI010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9818_length_5298_cov_3.656494"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLM83_06065"
     CDS             <1..514
                     /locus_tag="PLM83_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71005.1"
                     /translation="GNTQTIQIDPETIVYRNGVASDLESIRQNDSVYAVGTGYGATRF
                     VMAEGAVTREDITRKVSALTDGIITPEQVDAISRGKWDEARNGMSPALAQRLVEMGLT
                     EEEAQALLAEEWDALPELAQRRLAMALSEEIGVSPELIESIMNRDWKSARSYAELEVA
                     ELLLSRFLNM"
     gene            758..2191
                     /locus_tag="PLM83_06070"
     CDS             758..2191
                     /locus_tag="PLM83_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOK71006.1"
                     /translation="MLRLRHNVIWILASAVLLIGIASTFLRPTAIGAQKGADNSMELT
                     IYNSDLALVKETRCLEIPEGLSTVLFPNISARINPATVSFVSLTSPEEVRVREQNYEY
                     DLVEDTKLMQRFVGEKIQVTTKGGRAYTGYLLSGAKGSAPSNIIMSEGPNGTGQVYTV
                     QLSDVQSVEYPSLPEGLITRPTLAWLVSNNSTTRNHDCLVTYLTGGLSWAADYVAIIG
                     PNDDNLDLRGWVTVTNESGATFKDAKLKLVAGDVHQVQEEMYDVVRATAKVLDAGTGP
                     AFEEKPFFEYHLYTLQFPTTLKENQIKQIELLAASSIPARKLLVYDGARQGTKVTTML
                     EFWNKTENNLGMPLPKGVIRVSKMDTDGSLEFIGEDRIDHTPKDEEVRIELGSAFDVV
                     GERSVMGTKRLSDRVNEQTFKIEVRNHKEEPVEVTVLEHMMGWAQWEIISSSTKWNKV
                     DANTIEFPTKAPAGGSTTITYTVRYTY"
     gene            2564..>5298
                     /locus_tag="PLM83_06075"
     CDS             2564..>5298
                     /locus_tag="PLM83_06075"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOK71007.1"
                     /translation="MRPIPDSVLECKMSLTPEALTIMESRYLLKDKEGNIIETPEEMC
                     LRVAREAARAGAQYEGTRESEPERVKLFYWLLVNHLFSPNSPTWMNAGTESSQNAACF
                     VLPVEDSLPQIFDAVRGAALIHQSGGGTGFDFSNLRPHGDVVRSTGGVASGPVSFMAV
                     FNAATETIKQGGKRRGANIGILRVDHPDIESFITCKVENTALSNFNISVGITDAFMAA
                     VEADTDYELINPRTKSAVGTKRARDVLNLIVDHAWKNGEPGVIFLDRIEEGNPTPSLG
                     RITATNPCGEQPLLPYEACNLGSVNLQKMYREAPLTDEELAAALGTGIHQPLLTGEFG
                     EGCRELITDEMRHVDWSLLGTVVRASADFLDSIIDVSEYPLPEIDKMVRTTRKIGLGF
                     MGLADLMIMLKVPYASEKAELLAELIAKFITGEARAMSRRMAAERGPFPAFSESVFAE
                     TGEPPLRNATVTTIAPTGTISMVANASSGCEPLFGVAFTKRNILGGKSLTQVNSLFLA
                     DAKQRGIYSDELMERIANNGGSLAGIDGIPSDMAKLYQTALEIPWEWHVRIQAATQRG
                     IDNAVSKTINLPNNATREDVGAAYRLAYSLGCKGITVYRTGSREVEVISSKKAAVASN
                     AQPEPLTPRGRKPFTVDSTLWGKLQPIDRPETLCGITKRIATPSGTLWATLNMYDGKP
                     FEIFCQIGRAGTDIYAFTESIARLISLALRAGIDPSEIVRQLRGIGGSGQVGFGPNKV
                     LSVPDAIGRMLEQCIQSRYAGIEWATPPSRGVAGQTAPEDSWEAAGIDISPDEATVQG
                     RLLFIDQEEDTAVDHGDAAEDGASMDFEVGDLSPEPARQADNFEEEDPSASNNGVESS
                     ETNSHADGMLAMSDDFGGHMGDPDYTSSGHRVTRHDLCPQCGNYSLVYASGCSEC"
BASE COUNT         1394 a         1387 c         1423 g         1094 t
ORIGIN      
        1 cggcaacacg cagacgattc aaattgaccc cgagacaatc gtctatagga atggagtcgc
       61 gtcagacctg gagtctatcc gccaaaacga ctccgtatac gccgttggga cgggctacgg
      121 tgcaaccaga ttcgtcatgg cagaaggtgc agtcacacgc gaggacatca cgcgaaaagt
      181 gagcgccctc actgacggca tcatcacgcc agaacaggtg gatgccatat cccgtgggaa
      241 gtgggatgag gcccggaacg gaatgagccc agcactggcc cagaggctcg tagaaatggg
      301 cttgaccgaa gaggaagccc aggctctcct cgccgaagag tgggacgccc tgcctgaact
      361 ggctcagaga cgtctggcaa tggctctgtc cgaagagatt ggagtatcac ctgagctcat
      421 agagtccatc atgaatcgag attggaaatc cgcgcgcagc tatgctgagc tcgaggtagc
      481 agagcttctg ctctcaagat tcttgaacat gtaaaggtgc gccacccgaa cctggctgcc
      541 aacagacttg ttctcgtcgc taggcagcag gcaacatgtt tcgccgccgc gttggaacaa
      601 acagcgcggc ggcggcgtct cttcagagtg aaagcaggtt gtgtatgaat acgctggttt
      661 caggatatgg ccttctcatc ttaaaggaat caaaaacaag agacaagaag attgacatcc
      721 gaaactgagg caataactag tattggaggg tgctgtaatg cttcgcctac gacacaatgt
      781 catctggata ctggcatcgg cagtcctgtt aatcggaatt gcgtcgactt ttctacggcc
      841 cacggcgatt ggcgctcaga agggagccga taactccatg gaactaacca tatacaattc
      901 agacctcgcg ctcgtaaaag agacgaggtg cctcgagatc ccagagggcc tttccacggt
      961 gttgtttcct aatatatcgg cccggataaa cccggccact gtaagtttcg tctctctcac
     1021 atctccagag gaagtcagag tgcgcgagca gaactacgaa tatgatcttg ttgaagacac
     1081 aaaactcatg cagagatttg tcggtgagaa gattcaagtt accacgaaag gcgggcgcgc
     1141 ctacacagga tatttgctga gcggggcaaa aggatccgcc ccttccaaca tcatcatgtc
     1201 ggaaggtcca aacggcacag gccaagtcta caccgtgcaa ctaagcgatg ttcagtcagt
     1261 agagtatccc agccttccgg aaggcctgat tactcgtccc accctggcgt ggctggtgtc
     1321 taacaactcc acaacccgaa accatgactg cttggtaacg tacctcactg gtggactttc
     1381 ttgggcggca gactacgtgg ctatcatcgg cccaaacgac gataatctgg accttcgcgg
     1441 ttgggtcact gtgactaacg aatccggagc cacatttaag gatgcaaagc tcaaactcgt
     1501 ggccggagac gtgcaccagg tgcaagagga gatgtacgac gtagtaaggg caacagcaaa
     1561 agtccttgac gctggaacgg ggcctgcatt tgaggagaag ccgttcttcg agtatcacct
     1621 ttacactctt cagtttccaa caacactaaa ggaaaaccag atcaaacaga tagagcttct
     1681 ggcagcaagc tcgattccgg ctagaaaatt gctggtatac gacggtgcaa ggcaaggaac
     1741 aaaggtcacc acgatgctgg aattttggaa caaaacagaa aacaacctcg ggatgcccct
     1801 tccaaagggc gtgataaggg tcagcaagat ggataccgac ggtagcctgg aattcatcgg
     1861 cgaagacagg atcgaccaca ctccaaagga cgaggaagtc agaatcgaac tgggatcagc
     1921 cttcgacgtc gtgggagagc gctctgtaat gggcacaaag aggctttctg acagagtcaa
     1981 cgagcagaca ttcaaaatcg aagtgcgcaa ccacaaggag gagccagtgg aagtcactgt
     2041 tctcgaacac atgatgggct gggcccagtg ggagattatc agctccagca ccaagtggaa
     2101 caaagtagac gccaacacga tcgagttccc cacaaaggcg ccggctggcg gatcaaccac
     2161 gataacatac acggtaaggt atacctacta gtccaggatc agaccggttc gtaagctgaa
     2221 ttcgagggtc gctttcgggc gatcctcctt gttttctctg tcgaagccgg cgaaggcgaa
     2281 aatccagtca acttcaatcc gcttcctttg ttcactctct tttttcttgt gtccttggca
     2341 cactttacgt tcgctttatg ggttatcata ttgatgcatg ctgatgactc tactggctaa
     2401 cacaccccga ttcttccata tcccggagag ctggggcatg gaaatttcga tattctatat
     2461 cacatttcgc atgcaggaat caggttggcg gtgttgaaaa ctgagtcaat cctagagagg
     2521 agttctagga tcgtaaagac cctctcgaac aggaggaaga tagatgaggc ccatcccgga
     2581 ctctgtcctt gaatgcaaaa tgtctcttac ccccgaagcg ctgaccatca tggaaagccg
     2641 atatctgtta aaagacaagg aagggaacat aatcgaaacc cctgaggaaa tgtgcctcag
     2701 agtagcaaga gaagcggcaa gggctggtgc tcaatatgag ggcacgcgtg aatctgaacc
     2761 agaacgagtg aagctctttt actggcttct ggtcaatcat ttgttttcgc caaattctcc
     2821 tacctggatg aatgccggca ctgaaagctc gcaaaatgcc gcatgcttcg tcctacccgt
     2881 agaagattct ctgccccaga tatttgatgc agtccgcggt gccgcgctga tacatcaatc
     2941 cggaggcgga acaggatttg acttttctaa tttacgccct catggtgatg tcgtaagatc
     3001 gaccggagga gttgcctcag gtccggtctc tttcatggct gtgttcaatg ccgccactga
     3061 aaccatcaag caaggcggaa aacgccgagg cgcaaacata gggatactcc gagtggatca
     3121 tcccgatatc gaatccttca taacgtgcaa agtggagaat accgctctct ccaatttcaa
     3181 tatttctgta gggatcacag acgccttcat ggccgccgtg gaagccgaca ccgattacga
     3241 actcatcaat cccaggacga agtcggcagt gggaacgaag agggctcgcg acgtcctcaa
     3301 cttaattgtg gaccacgcgt ggaagaatgg agagccaggc gtgatattcc tagatcgaat
     3361 tgaagaaggc aaccccaccc cttcgctagg ccgcatcacc gccacgaatc cctgtgggga
     3421 acagcctttg ctgccttacg aggcatgcaa ccttggcagc gtgaacttgc agaaaatgta
     3481 ccgcgaggcg ccactgaccg atgaggagct tgctgctgcc cttggcacag gaattcatca
     3541 accattgctc acgggcgagt tcggagaggg ctgccgcgag ttaatcacag atgagatgcg
     3601 ccatgttgac tggagtctcc ttggcactgt ggttcgcgct tcagcggatt ttctggattc
     3661 tataatagac gtaagcgagt atcccctccc agagatcgac aagatggtaa ggacaaccag
     3721 aaaaatcggt cttggtttca tgggcctcgc agatcttatg atcatgctca aagtaccgta
     3781 tgcatcggaa aaagcagaac ttcttgccga gctcattgcc aagttcatca cgggtgaagc
     3841 acgggcgatg tcgaggcgga tggctgcaga acgagggcca ttcccagcgt tttccgaaag
     3901 cgtattcgcc gaaaccggag agcccccgct tcgtaatgcc actgtcacta cgattgcgcc
     3961 aacgggcaca ataagcatgg tagcaaacgc ttcttcaggg tgcgagccct tgttcggcgt
     4021 ggcattcacc aagagaaaca tactaggcgg caagtctctc actcaagtca actcgctgtt
     4081 tcttgccgac gccaaacagc gcggcatata ctcggacgag ctgatggaac gcattgctaa
     4141 caacggcggt tcgctggcag ggatcgatgg catcccctcc gatatggcaa agctatacca
     4201 aacagcattg gagatcccat gggagtggca tgtgcgaatt caggctgcaa cacaacgagg
     4261 catagacaat gcggtatcga agactattaa cttgcccaac aacgccaccc gcgaggatgt
     4321 tggcgcagct taccggctcg cctactccct cggctgcaaa ggcataacag tatatcggac
     4381 cgggagtcgc gaggtagagg taatctcgtc caaaaaggct gcagttgctt cgaacgctca
     4441 gccggagccc ttgacgccta gaggacgcaa gcctttcact gtcgattcca ccctctgggg
     4501 gaagcttcaa cccatcgaca ggcccgaaac actgtgcggc ataacgaaga gaatcgctac
     4561 accatccgga acactctggg ccacgctaaa catgtacgac ggcaagccat ttgagatatt
     4621 ctgccagata ggcagggccg gcaccgacat atatgcgttc actgagagta tcgccaggct
     4681 aatctcgtta gctctccgtg cagggataga cccatctgaa atagtgcgcc agctcagagg
     4741 cataggcggt tcaggtcagg tgggattcgg tcccaacaaa gtgctctcgg taccggacgc
     4801 cataggtcga atgttggagc agtgcattca gtcgcggtac gccggcattg aatgggccac
     4861 gcccccaagc cgcggcgttg cagggcaaac agctcccgaa gacagttggg aagcagcggg
     4921 aatcgatatc tcgcccgatg aagccacggt ccaagggcgg ctcctcttca tagaccagga
     4981 agaggatacc gcagttgacc atggggatgc tgcggaagac ggagcatcca tggattttga
     5041 agtcggagac ctctctccag aaccagcgcg gcaagcggat aactttgaag aagaggatcc
     5101 ctcagccagc aacaatggtg tagaatcatc cgagaccaac agccatgcag atggaatgct
     5161 tgccatgtct gacgatttcg gaggacacat gggcgaccca gactacactt catccgggca
     5221 tcgcgtgaca aggcatgatc tatgtcccca atgtggaaac tactcgctgg tatatgcgtc
     5281 cggctgctcg gaatgtag
//
LOCUS       DAOSAI010000149         5291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9833_length_5291_cov_3.109053, whole genome shotgun sequence.
ACCESSION   DAOSAI010000149 DAOSAI010000000
VERSION     DAOSAI010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9833_length_5291_cov_3.109053"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLM83_06080"
     CDS             complement(<1..846)
                     /locus_tag="PLM83_06080"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOK71008.1"
                     /translation="MQVLFFSPGEADDRTRLDLYLAKESVAPSRSYIQKLISDGRVTV
                     NGLVETRSSYRVRAGDAVEVRVPPPEPLCPGVPAPESIALDVIYEDDDLMVINKPRGM
                     VVHPGVGRSTGTLVNALLGRGTQLSTVGGKLRPGIVHRLDLDTSGAIIVAKNDATHLA
                     LASQLASGDIDRNYIAIVRGNIREDCGVIDAPLGRHPVDRKKRAVRPDGRPAVTRFTV
                     LERFGDYTLVGIELETGRTHQIRVHMKYIGRPVAGDPVYGGVAGELGLEAQALHARSV
                     EFTHPV"
     gene            complement(850..1644)
                     /gene="lgt"
                     /locus_tag="PLM83_06085"
     CDS             complement(850..1644)
                     /gene="lgt"
                     /locus_tag="PLM83_06085"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOK71009.1"
                     /translation="MHPILFNVGKFPVYSWGTCLAVAFVVSALYAIWQGKKKEIDPDD
                     IIDLALYACIASILGARIFYVLLNITSYLDNLVSILWMRDGGLSFFGGLAGGVLAGVL
                     LAKKREIPLGKLADVVAPAIAIGYAIGRLGCLLNGCCYGKVAVDLPWALACGTDGAPR
                     HPTQIYAIIYSVIIFFILRAWEGRKRFEGEVMWAYIGLYSVARFIVEFWRVGVRDYFA
                     PLTATQMLCIVLAVLGFGMVYVKSKYPESVAEASTDGAVSKSSVRG"
     gene            complement(1644..2093)
                     /gene="lspA"
                     /locus_tag="PLM83_06090"
     CDS             complement(1644..2093)
                     /gene="lspA"
                     /locus_tag="PLM83_06090"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOK71010.1"
                     /translation="MSLFLTSAWVILLDQITKTIVTRQIAPWDNIECLGGLLQITNVH
                     NPGAAFGILQNKQILSIVAAIVAAVLILLFYPRIPRHRRSMRFGMALALGGALGNLID
                     RVKYGSVIDFIDVGFWPVFNLADSAIVVGGALILLGMARDPSVRRSN"
     gene            complement(2185..2751)
                     /locus_tag="PLM83_06095"
     CDS             complement(2185..2751)
                     /locus_tag="PLM83_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOK71011.1"
                     /translation="MEPEKIDSCKSRLIELKAQAESRVRDVEETAGFDTPESEWSGEL
                     SSYDNHTADSASALEMRSINLGLIHHGRDIVAQIESALERIADGSYGKCDQCGRDIPP
                     ERLDAIPWTTSCAQCKADSLSPSGRSGPRPIEEEVVRPPFGGRDPARAYDDPGIHGED
                     IWDALAEYGTANSPQDEEAGNSGDVDSF"
     gene            complement(2768..3067)
                     /locus_tag="PLM83_06100"
     CDS             complement(2768..3067)
                     /locus_tag="PLM83_06100"
                     /inference="COORDINATES: protein motif:HMM:NF040137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HOK71012.1"
                     /translation="MRRKRRPGEVRALMSQIMQLSRRLEDSSVAEYVEFMRNPRRVIW
                     SNFLAGMARGFGIAIGASVLTALFLYALSVVISLNIPIIGNLIAKIVKIVKDSGV"
     gene            complement(3112..3612)
                     /locus_tag="PLM83_06105"
     CDS             complement(3112..3612)
                     /locus_tag="PLM83_06105"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOK71013.1"
                     /translation="MLTPLEVHSKQFQTKFGRYNAAEVDDFLDLVGQCYDQLYRENAE
                     LKEKLRLLSEQAQYEPEERDEDIAETIKQTLLMAQKAAEDARRNAEEKASLIVENAER
                     EAAAVLDRTEERVRAQQKRLEDLYAQEAAARARFKAMLEAYLEMLEDTHRDAEGFVGG
                     VTRKYE"
     gene            complement(3626..4387)
                     /locus_tag="PLM83_06110"
     CDS             complement(3626..4387)
                     /locus_tag="PLM83_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOK71014.1"
                     /translation="MYDDSAVLRAAREAAQSGVPVRLGFIDPNEQDDAAEVLASIGDV
                     TPRFYGGYRGAARKLLVIMPSGYLDEVFNAGISYFGLRGVGLSLDGGSGSDAAIAELR
                     ALGIPRDHIGDVFYGGNEWQGVVLASVFGDGSNERELEGSGARLVELDPAEVVFPGVV
                     PKKIRATVASMRLDAVAGAGFPASRSRLAQEIKAGRFKVNGEIVQSPSVKVSEGDVVV
                     SRGRGRLVVEEVFGETKRGRVSLMLTRYSANAPFS"
     gene            complement(4433..5134)
                     /locus_tag="PLM83_06115"
     CDS             complement(4433..5134)
                     /locus_tag="PLM83_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOK71015.1"
                     /translation="MSSIADNLGILQDRIAAAVGRAGRDPSEITVVGVTKNVEAQRIA
                     EAVAVGIRDFGENRVQEAAKKIPLVEADVSWHLVGHLQRNKAKQAVELFSLIHSLDSA
                     RLAEELNRRCEMADCVIDLLLQVNVSGEDTKFGIPPEMARDALLEMAKYERLRVCGLM
                     TIAPYSDDPEDARPCFARARKLWEDLGKLSISNVRMDELSMGMSGDYEVAIEEGATII
                     RVGTAIFGPRRRELS"
BASE COUNT         1263 a         1726 c         1202 g         1100 t
ORIGIN      
        1 cacaggatga gtaaactcca cactgcgggc gtgaagagcc tgagcctcaa gacccagttc
       61 gcccgcaaca cctccataga caggatcacc tgctacaggc ctacctatat acttcatgtg
      121 gacccgaatc tggtgtgttc gacctgtctc cagctcaatc ccgaccagag tatagtcacc
      181 aaacctctcc aaaacagtaa acctagtcac cgccggcctt ccgtcgggcc tcacagctcg
      241 cttcttcctg tctacgggat ggcgccccaa aggcgcatca atgaccccgc agtcctcccg
      301 gatgttcccg cgcacaatag caatgtaatt gcggtctatg tcgcccgatg ccagctgcga
      361 cgcgagagca agatgcgttg cgtcgttctt tgccacgata attgcacccg atgtgtcgag
      421 gtcaagacgg tggactatcc caggcctgag cttccctcca accgtcgaca actgagtgcc
      481 cctaccgagc agagcattga ctagagtgcc ggtagacctg cccacaccgg gatgaacgac
      541 catcccccgg ggtttgttga tcaccatcag gtcatcatcc tcatagatca catcgagggc
      601 gatcgactca ggcgcgggaa ctcccgggca caggggttca ggcggcggaa ctctcacttc
      661 gacagcatcg ccagcacgga cgcgataaga agagcgtgtt tcgaccaagc cattaacagt
      721 gactcgtcca tccgaaataa gcttctgaat ataggaacgc gaaggagcaa ctgactcctt
      781 cgcgagatac agatcgagcc ttgtgcgatc atcagcctcc ccgggactga agaacagaac
      841 ctgcatcatc taccctcgca cagacgattt cgacactgcg ccgtcagtcg aagcctcggc
      901 gaccgactcg ggatacttcg acttcacata taccatgcca aatcccaata ccgcaaggac
      961 gatgcatagc atttgggtgg ccgtcaacgg agcaaaatag tcgcgaaccc cgactctcca
     1021 gaactcaact ataaacctag caacagagta aagccctata tacgcccaca ttacttcgcc
     1081 ttcaaaacgc tttctgcctt cccaagcccg aagaatgaag aaaataatga cactgtagat
     1141 tatcgcgtag atctgcgtag gatgcctagg agctccgtcc gttccgcatg ccaaagccca
     1201 tggaagatcc accgcaacct tcccgtagca gcatccattg agaagacagc ctagccttcc
     1261 tatggcatag ccaattgcta tagcgggcgc gaccacgtca gccaatttcc ccaaaggaat
     1321 ctcgcgcttc ttggccaaaa ggacccctgc gagcaccccg cctgcaagcc cgccaaagaa
     1381 ggaaagccca ccatcgcgca tccacaagat gctgacgaga ttgtcgagat aactagtgat
     1441 attgaggagc acatagaaaa tcctggcgcc cagaatcgac gcaatgcagg cgtacagcgc
     1501 gaggtcgatg atatcatcgg gatctatctc cttcttcttg ccttgccaga ttgcgtagag
     1561 ggcgctgaca acgaaggcaa cagccaaaca tgtgccccaa gagtatacgg gaaacttccc
     1621 aacattaaac aggatcggat gcatcagtta ctcctcctta cactggggtc gcgcgccata
     1681 cccaaaagga tcagtgcgcc tcccacaacg atggcgctgt ctgccaggtt aaacaccggc
     1741 cagaagccga cgtcgatgaa atctatgaca cttccatact tcaccctgtc aataaggttc
     1801 ccgagcgccc cgcccagcgc cagcgccatt ccaaacctca tcgaccgcct gtgcctggga
     1861 attctgggat agaaaagcaa tatcagcacc gctgcgacaa ttgcggcaac aatcgacaat
     1921 atctgcttgt tctggagtat tccaaacgcc gcccccgggt tgtgcacatt tgtgatctgc
     1981 agcagcccgc caaggcactc gatattgtcc catggcgcta tctgcctcgt cacaatcgtc
     2041 ttagttatct ggtcgagaag gataacccat gcacttgtca ggaaaagcga cacatgcctc
     2101 caccccaata atctctctca agtctgaact caattctagc acacgaaatg tgccccgtaa
     2161 acactctaac atgccacgca actactaaaa cgaatccacg tcgccagagt tcccggcctc
     2221 ttcatcctgg ggactgttgg cagtgccata ctccgctagc gcatcccaga tatcctctcc
     2281 gtggatgcca gggtcgtcgt aggcgcgggc aggatccctg ccgccgaacg gaggcctcac
     2341 aacttcttcc tcgataggcc taggaccgct tcttccggaa ggtgacagag aatcggcttt
     2401 gcactgagcg caactggtcg tccacggtat cgcgtcgagg cgctcgggag gaatatcccg
     2461 accgcactgg tcgcatttgc cataagaccc atccgcgata cgctccagtg cagattctat
     2521 ttgggcgact atatcccgcc cgtggtgtat caagccgagg ttaatagatc gcatctccag
     2581 cgcagaagcg ctatctgccg tgtgattatc gtaactcgaa agctcgccgc tccactccga
     2641 ttctggcgtg tcgaaacccg cagtctcctc aacatcccgc accctcgatt ccgcctgcgc
     2701 cttcagttcg ataagcctgc tcttgcacga atcgatcttt tcgggttcca tctgcgcacc
     2761 tcccgcgtca tacgccgcta tccttcacta ttttgacgat cttggcgatt aggttgccta
     2821 tgattggaat attaagcgag atgacaacgg aaagtgcata taggaatagc gcggtaagga
     2881 cggacgcccc tatagcaatg ccgaatcccc tagccattcc agcaaggaag ttcgaccaaa
     2941 tgacccgccg cggattgcgc ataaactcga catactccgc aacgctagaa tcctcgaggc
     3001 gccgagagag ctgcatgatt tgggacatga gggccctgac ttcgcccggc cgccgcttcc
     3061 ttcgcacaga tcacacctcg cgaaggatat agtgcccacg tccgaacggt tttactcgta
     3121 ttttcttgta actcccccca cgaacccctc cgcatcccgg tgcgtatcct ctaacatctc
     3181 gagataggcc tcgagcattg ccttgaacct ggccctcgca gcggcctcct gagcgtagag
     3241 atcctccaaa cgtttctgct gagcacggac cctctcctcc gtccgatcga gcacggcagc
     3301 agcttccctc tcagcatttt ccacaatgag cgaagctttt tcctccgcgt tgcgcctagc
     3361 atcttcagct gccttttgag ccatcagaag agtctgttta atcgtttcag ctatgtcctc
     3421 atcgcgttct tccggctcat actgggcctg ctcggaaagg agtcgcagct tctccttgag
     3481 ttcagcattt tcgcggtaca actgatcgta gcactgtccc accagatcga gaaaatcatc
     3541 gacttccgct gcattgtaac gcccaaattt cgtttggaac tgcttagaat ggacttcaag
     3601 tggcgtaagc attccgaccc tcctctcaag agaatggcgc gtttgccgaa taacgtgtca
     3661 acatgaggga aacacgccct cgcttggttt ccccgaaaac ttcctccaca acaagcctgc
     3721 ctctcccgcg agagactacg acatcccctt cactaacctt gacagaagga gactgaacaa
     3781 tctccccgtt taccttgaac ctgcccgcct tgatctcctg ggcaagcctt gacctcgatg
     3841 ccggaaatcc agcgcctgcc accgcgtcca gcctcatcga agccaccgtg gccctgatct
     3901 tcttcggaac aaccccggga aacaccacct ctgcgggatc cagttccacc agtctggcgc
     3961 ctgatccctc tagctctcgc tcattgctcc catccccgaa gacggatgcg agcaccacac
     4021 cctgccactc gttgccccca tagaacacgt ctccgatgtg gtcacgtgga atgcccaagg
     4081 cccgaagctc ggcaatggcc gcatcagacc cggatccccc atcaagactg agcccgacgc
     4141 cacgcagccc aaagtaggat atgcccgcat tgaaaacctc gtcgagatac ccagaaggca
     4201 taatcacaag cagcttacgc gccgcacccc tgtatccccc gtaaaatcga ggcgtcacat
     4261 ccccaattga cgcaagcact tcggccgcat cgtcctgctc gttcggatca ataaatccca
     4321 gccgcacagg aacacctgac tgggctgcct cgcgcgcggc gcggagcaca gcagaatcgt
     4381 cgtacatgca agcacctcca tacaaccggc tcgcagtggg aaatgccgga tatcacgata
     4441 actcgcgccg cctaggcccg aatatcgccg tgcccaccct gataatcgtc gcgccctctt
     4501 ctatggccac ctcgtaatca ccggacatcc ccatggacag ctcgtccatt ctcacgttgg
     4561 aaatcgaaag ctttcctaga tcctcccaaa gctttcgagc ccgagcaaaa catgggcgag
     4621 catcctccgg atcatcagaa tatggggcaa tagtcatcag tccgcaaacc cgcaggcgct
     4681 cgtatttcgc catttcaagc aaagcatccc gggccatttc aggcgggatg ccgaacttcg
     4741 tatcctcgcc ggagacattg acctggagaa ggagatctat aacgcaatca gccatttcac
     4801 atctgcgatt gagttcctca gccaacctag cgctatctag agaatgtatg agagagaaaa
     4861 gctccacagc ctgcttagcc ttgttccttt gaagatgccc caccagatgc caactgacat
     4921 ccgcttccac aagcgggatc ttctttgcag cctcctgcac cctgttttcc ccgaaatccc
     4981 gaatccctac ggccactgcc tcagcaatcc gttgagcctc gacattcttg gttacaccca
     5041 ccacagtaat ctcagagggg tcgcgacccg cgcgccccac agctgcggca atcctgtctt
     5101 gaagtatccc gagattatcg gcaatcgacg acacaaaacc cacctcatcc taggctacgt
     5161 attctccgaa ttctaaccta ttcctccata ccttccccac ccagacaggg aatgtaccgc
     5221 cgctatttcg gcatccgcgc actacggccg ccatcgccga gctttaccct atctccttcg
     5281 ctcaggccag c
//
LOCUS       DAOSAI010000150         5272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9877_length_5272_cov_3.279854, whole genome shotgun sequence.
ACCESSION   DAOSAI010000150 DAOSAI010000000
VERSION     DAOSAI010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9877_length_5272_cov_3.279854"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1240)
                     /locus_tag="PLM83_06120"
     CDS             complement(35..1240)
                     /locus_tag="PLM83_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK71016.1"
                     /translation="MKVKRLFLSVVWGALILSLAIIPSMAADPLVVWCEPQKMGTIEP
                     LADQWSKKTGIPVKVEPVGVLETANKVQLAGPMGKGPDVFCSVSGSLGMLVVTGTVAP
                     VNPKTFDLSNFMKVSVDAATFNGKLYGVPYDISTVAMIYNKKIWPEPPKTFNEMIDRS
                     RELRKKGKFGILWPMESFYFSYAIMAGYGGDIFAPPESGWDTKDIGLANPGSVKAVKL
                     LKTLRDEGIIPVGTDHVVAPARFSEGNAAAIIDGSWALPGIKQAGIDYGVAPLPKLDN
                     DKYPAPFVSLKWWHISSYSQRKTDAANLISYLTTKEAMYKSFKAAEGIPPRLDVLQMP
                     DVKSMPEVAGYGAQANYGSILPDIPEVNPVWAPMDSALEIAIRGDKSVEAALSDAVKI
                     IRQNIAELK"
     gene            1791..2534
                     /locus_tag="PLM83_06125"
     CDS             1791..2534
                     /locus_tag="PLM83_06125"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK71017.1"
                     /translation="MKVCDCHCHVGEFYDQINKKHVSYSPAELVKCMHEADVDLAIVS
                     NISAVDAPMQANFELAEWVEEYRELVPIAWTTPEVTKVGEVRQLLALGFRGLKFHPTA
                     GGYRADSNEVDRYLRICAQEGKPALFHCAADESSAPERYMALAERNPDVWIILAHMNL
                     FGPAEAAIRVAEAYPNICLDTSWARPEDVKDAILRIGAERILWGTDAPLGGECHYRRD
                     RVRAALESKLDEGELKSVMWGNAARLFSL"
     gene            complement(2689..3453)
                     /locus_tag="PLM83_06130"
     CDS             complement(2689..3453)
                     /locus_tag="PLM83_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71018.1"
                     /translation="MDDFLLKQTVLTLKPEPVPAEDVLRSLGMSDVIVKLEADNCLPP
                     DISNDVALLATLAAIDEANRAVIDGLVVPRVVATTLPIASIADKGIFLDSIAENSSAF
                     IPCSGLLFEGASHVMLAIATIGPLLERQVKAEFDTGDPLAGIVLDAMGTISLQRLASR
                     IRQTCAQRADECGMETGPRIAPGCEAMPLDAQSALFSLLDADAIGVTLSDSLLMMPLK
                     STSLVLPLGPSLPDHLTRFNMCDTCPHKNRCANIIL"
     gene            complement(3522..>5272)
                     /locus_tag="PLM83_06135"
     CDS             complement(3522..>5272)
                     /locus_tag="PLM83_06135"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOK71019.1"
                     /translation="VRLACQAAILGDADVTVLSRVAPGNQNASILTDSKWLDNQVESA
                     DARVEAKTVSIDPTSSARSDWERISLAFGLPKYCQIADGILSECPEPASIPTLKLAQS
                     VGEAMAEATSPAQSAGTSSSSIVPCADNGIALTGVFIDGKLADVLAATAKPTRRSESG
                     GRHPLGIALDIGTTTLVGYLVNLATNTVIGVKGTVNPQTAHGADLISRIAYSQSKEGL
                     ETLRLEVIEAVNELANGLLADADASPDDVYLVGAVGNTCMHHLFCGISPSRLARLPYA
                     PAVLESAPCSPAEVGLACANPRGKFFFLPNIAGFVGSDALAVALVSGMSESSAPTLAI
                     DIGTNGEIMLAANGRILACSTAAGPAFEGVNISCGMIAAPGAIDGVRMGKDDIECQVI
                     GDASPRGICGSGLISLIAVLREAGMISETGAFDPDRVDPGSPLRSRLIKDQAGLQFVL
                     AKSGSQGPDVVLTQRDVRELQLAKAAIRAGVEILLAEIGIEAEELSQIVLAGAFGTYL
                     DTEAATAIGLLPDAGDARLVSLGNAAGQGVISALVSAKAYREAQNLAKTIEHVELGAS
                     PLFMEAFTDSMLLSRG"
BASE COUNT         1130 a         1371 c         1521 g         1250 t
ORIGIN      
        1 gttgtgtcaa attctgttcg gtaactgccc ggctctattt gagctcagct atgttttgtc
       61 tgatgatctt aaccgcatcg gaaagagctg cttccacact cttatcgccg cgtattgcga
      121 tctcaagtgc gctgtccatc ggtgcccata cgggattgac ttccggtatg tcaggtagta
      181 tcgaaccgta gttggcttgc gcgccgtagc ctgccacttc aggcatcgac tttacatcgg
      241 gcatctggag gacgtcaagc cgcggaggga taccttcagc ggccttgaag gacttataca
      301 tggcttcctt agttgttaga taggagatga ggtttgcagc atcagtcttg cgctggctat
      361 acgagcttat gtgccaccat ttcaggctta caaacggcgc tggatacttg tcattgtcaa
      421 gctttggaag cggagctaca ccgtaatcga taccggcctg cttgattccg ggcagtgccc
      481 aggagccatc gatgatggct gcagcgttgc cttcactgaa tcttgcaggg gccacgacgt
      541 ggtcggttcc gaccgggatg atcccttcat ctctgagcgt cttcagaagc ttgacggctt
      601 taacagagcc tgggttggca aggccaatgt ctttcgtatc ccatcctgac tcggggggcg
      661 caaagatgtc cccgccgtat cctgccatta ttgcatagga gaagtagaag ctctccattg
      721 gccacaggat tccgaatttg ccctttttcc gaagctcgcg ggatctgtcg atcatctcgt
      781 tgaaggtttt gggcggttca ggccaaatct tcttgttgta tatcattgct acggtggaga
      841 tgtcgtaggg cactccgtac agcttgccgt tgaatgtggc agcatcgacg cttaccttca
      901 tgaagttgga cagatcgaag gttttagggt ttactggggc aactgttcct gtgacgacta
      961 acatgccgag cgaacccgaa acgctgcaga acacgtcagg ccctttgccc attggcccag
     1021 ctaactgcac cttgttggcg gtttccagga cgccaacggg ttcaaccttc actggtatgc
     1081 ctgtcttttt gctccactga tcagcaaggg gttcgatagt tcccatcttc tgcggctcgc
     1141 accagactac caagggatct gcggccatag acgggatgat ggcgagtgat agaatcaacg
     1201 caccccaaac gacggagaga aacagacgtt taaccttcat gctaactttc ctcctctgtg
     1261 gtttcagtat tgagtgaaac tcgtaaccac gcccgtgggt ggcaagtgcg ccagtatggc
     1321 ttgctcgcag caacaaattg gctttatcag attgcgcccc gcatgagaac ggagacaatg
     1381 tgttttcggt cgacaaaaac aggatgatga gttgagatat gatttcatgt cgtgctcgaa
     1441 attgcaccgg ttgaggatcg gtccgaaaag ggctagcagt gaaggaaagg tgtatattag
     1501 tgtcgggtac ccagctgaaa ccattgtgga tccgatccat atacttgctc gaatcgggct
     1561 ttgcaacaac agatcccgtg gagcatgaga gcattccgct tgctcggacc tgagtttgag
     1621 ccagggatta tgccgcctac ggatttaagc tgcatggatg cctagtagtc atcctaacga
     1681 ctactataat aatattagca tatccattcg atgtcaatag caccattgaa aacccgaagg
     1741 ctgtaataga aaggcatgtc cgtcacggcg gcctgaactg gagggattgt atgaaagtct
     1801 gcgattgcca ttgccatgtc ggcgaattct atgaccagat aaacaagaaa catgtctcgt
     1861 attcccctgc cgagctcgtt aaatgcatgc atgaggcgga cgtcgatctc gcgattgtat
     1921 cgaatatctc agctgttgac gctcctatgc aggctaattt cgagttggct gagtgggtcg
     1981 aagagtatcg ggagttggtg ccaattgcgt ggactactcc tgaagtgacg aaggtaggtg
     2041 aagtgcgaca acttcttgcg ttggggtttc gcggtctgaa gtttcatccg actgcaggag
     2101 ggtatcgggc cgattcgaac gaggttgaca ggtatctccg aatctgtgcc caagagggaa
     2161 aaccagctct gtttcactgt gctgctgacg agtcctctgc gcccgaacgc tacatggcac
     2221 ttgctgaacg caatcccgat gtgtggatca tacttgccca catgaaccta tttggacctg
     2281 cggaagcagc tatccgtgtg gctgaggcct accctaacat ctgcctcgat acaagttggg
     2341 ctaggcctga agatgtgaag gacgcgattt tgcggatcgg ggctgagagg attctttggg
     2401 ggacagacgc tccgcttggc ggggaatgcc actacaggcg ggaccgggtc agggctgcgc
     2461 tcgagtcgaa actggacgaa ggcgaactca agtcagtgat gtgggggaat gcagcaagac
     2521 tgttctcgct ataagtacat ttgccagttt ccgagcgttc ttaagaggcg ccgccttatt
     2581 gtggataacc gggtgtccaa ttgtcgtcac ctgcctgggc aggctggtaa taggtgagaa
     2641 aatttgagat agggtaaggt cactgatcgg cactgattcg aattagcatc aaaggattat
     2701 gtttgcgcat ctgtttttgt gaggacatgt atcgcacatg ttgaatcttg tgagatggtc
     2761 ggggaggctt ggccctaatg gcaagaccaa cgaagtcgat ttgagcggca tcatcaagag
     2821 tgaatcgctg agggttacgc caatggcgtc ggcgtcaagc agagagaaga gggccgactg
     2881 cgcatctaaa ggcatagcct cgcatccggg cgctatacga ggacctgtct ccattccgca
     2941 ttcgtctgcg cgctgggcgc aggtttggcg gatacgcgat gcaaggcgtt ggagggagat
     3001 agtccccatg gcatcgagga cgattcccgc aagcgggtcg ccagtgtcga actccgcttt
     3061 cacttggcgc tcgaggaggg gccctatggt agctatcgct agcatcacat ggcttgcacc
     3121 ttcgaacagc aggccactgc aggggatgaa cgcgctgcta ttctcggcga ttgagtcaag
     3181 gaagatgcct ttgtcggcta tggaggctat cgggagagtc gtggccacta cgcgcgggac
     3241 gacgaggccg tcgatgacgg ccctgttagc ctcatcgatg gcggctagcg tggctaacaa
     3301 ggcgacatcg ttgctaatat ccggaggcag gcagttgtca gcctcgagct tgacgattac
     3361 gtcggacatt cccaaggacc tgaggacatc ctccgcagga accggttcgg gttttaaagt
     3421 cagtaccgtc tgtttcaaga ggaaatcatc cattcgatcg atgcctccgt ctgttctgct
     3481 gggctcgtgt gagcgcttgg ctctaccctg agccccagct gttatcctct ggaaagaagc
     3541 atggagtcgg tgaatgcctc cataaatagg gggctggctc cgagttccac atgctcgatc
     3601 gtttttgcca ggttttgcgc ctccctgtat gctttagcgc ttaccaatgc tgaaattacg
     3661 ccttggcctg cggcgtttcc caaagagacc agacgggcat cgcccgcatc aggaagcaaa
     3721 ccgatggcag tggcggcttc ggtatcaaga taggttccga aagctccggc aaggactatc
     3781 tgcgaaagct cttccgcttc tatcccgatt tctgcaagaa gtatttcgac tcctgcccta
     3841 atggcggcct tggccaactg cagctcgcgc acatcgcgct gggtcaggac tacatctggg
     3901 ccttgtgacc ccgatttggc caacacgaat tggaggccgg cttggtcctt gataaggcgg
     3961 cttctaagag gacttcccgg atcaactctg tccgggtcga aagcccctgt ttccgagatc
     4021 atcccggctt cgcgaaggac tgcgatgagg cttatcaatc ccgaaccaca tatcccccga
     4081 gggctcgcgt ctccgattac ttggcactcg atgtcatctt tgcccatgcg cacaccgtct
     4141 attgcccccg gggccgcgat cattccacag gatatgttga ctccctcgaa ggcgggccct
     4201 gcagcagtcg agcacgcgag gattcttccg ttcgctgcga gcatgatctc gccgtttgtg
     4261 cctatatcga tagcaagtgt cggggctgag ctctcactca tgcctgaaac gagtgccacc
     4321 gccagagcgt cggatcctac gaacccggcg atgttgggca aaaagaagaa cttgcctctg
     4381 ggattggcgc acgcaaggcc tacttccgca ggcgagcatg gggcggattc cagaactgcc
     4441 ggagcgtaag gaagccttgc cagcctactc ggggagatac cgcagaacag atggtgcatg
     4501 catgtgttcc cgacggcccc cactaagtag acgtcgtcag gcgacgcgtc tgcgtcagcc
     4561 aaaaggccgt tggcaagctc gttcacggcc tcgatgacct ctagccttaa agtctcaagc
     4621 ccctctttcg actgcgagta ggcgatgcgg gagatcaggt cggcgccgtg ggcggtctgc
     4681 gggttgacgg ttcctttgac gccgattacg gtgtttgtcg ccaagttcac caagtacccc
     4741 actaaggtgg tagtgccgat gtccagcgcg atgccgagag gatgccgccc gccactttca
     4801 ctacgccgtg ttggtttggc tgtcgcagcg agaacatctg ccagttttcc gtcgatgaag
     4861 acgccggtca gggcgatgcc gttgtcggcg caggggacga tggatgagga ggatgtgcct
     4921 gccgactgag caggactcgt ggcttcggcc attgcttctc cgacagactg ggcaagtttg
     4981 agggtcggga ttgaggctgg ctctgggcac tcagataaaa tcccgtcggc gatttggcaa
     5041 tactttggca acccaaaagc tagtgagatc cgctcccagt ctgaacgggc agaagacgta
     5101 gggtcaatgg atacggtttt cgcttcaacg cgcgcatcgg ctgactcaac ctgattgtcc
     5161 agccatttgc tgtccgtcag tatggaggcg ttttgattgc ccggcgcgac ccgggacaag
     5221 acggttacat ccgcatcccc caagatagca gcctggcagg cgagcctcac gc
//
LOCUS       DAOSAI010000151         5241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9947_length_5241_cov_3.474740, whole genome shotgun sequence.
ACCESSION   DAOSAI010000151 DAOSAI010000000
VERSION     DAOSAI010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9947_length_5241_cov_3.474740"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2086
                     /locus_tag="PLM83_06140"
     CDS             <1..2086
                     /locus_tag="PLM83_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK71020.1"
                     /translation="ALKAKQDYKRGSFTNRRLYVNGDIPFAEVDAIRFPYAINYEMRV
                     LGDDAGEPYLFYLHEGVNEIRLEPVLGDLGPMLLRAEAALYDMNTVYRKIVMITSTNP
                     DPMRDYQLEERVPELISKLAELSEVMRCIGEQLEAYTGARGDYVALLYELARQLGSMA
                     KKPESIPKRIAELRDAGASLGSWILSTREQPLMIDYIVVASIEQEMPKAKPTWLQRFK
                     HEVKAFLASFVHRYDLVGNVYSTENGDGVRPLKVWIGAGRDQAQALKSMIEDSFTPAT
                     GIPVNLELVSMGVLLPATLSGNGPDVALGLDPAQPLNFALRGAVVSLTQFPDYPEVAA
                     RFRPSAMTPFSFRDQVYALPEQQPFPVMFYRKDILREMGISLPETWDDLIGIIPELQK
                     SHMDIGLPVSGTGAVGAGNVGEAVNAGSVSSNAGVMTFLTLLYQNGGRLYGPDGLCTG
                     LDDEEAVDAFTRWTELHELYTVPLDYNAINRFRIGEMPILITGYTLYNTLSVFAPELR
                     GEWGFTLVPGTRRPDGSIDRSCPAGSCGAGGVPGLASSVASGPATVIMSSAPDLEGAW
                     EFVKWWTSTETQVRFGREMESLMGPSARYPSANVDAVQELPWKSDECQMLMEQWEWVR
                     GIPEVPGGYMVGRHLDNAFRRVVYHHEPPREVLLDYNRMINEEITMKRLEFGLPVRYD
                     ELEAEWCSFDWQ"
     gene            2108..3034
                     /locus_tag="PLM83_06145"
     CDS             2108..3034
                     /locus_tag="PLM83_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK71021.1"
                     /translation="MTTVATDAELREPHGLGWKIKKHRVSYLFVAPYATFFFLFTVLP
                     VAVAIVLSFTDYNMLESPKWIGWDNYLRLFLVDDVYLIAVKNTFIFAAITGPLSYFLC
                     LIIAWLVNELPPKLRAALTLLFYAPSISGSLFLMWTIIFSGDAYGIINGILLYWGIID
                     QPIQWLTDPKYMMTVVIIVTLWMSLGTSFLVFIAGLQNVDKSLYEAAAVDGIKNRWQE
                     LFFITLPAMKPQLMFGAVMSITSSFAAAGQIIPLVGFPSTDYAAHTVVAHLLDYGNIR
                     FEMGYACAIAVILFVTMVVVQQIVQRILRNLG"
     gene            3058..3912
                     /locus_tag="PLM83_06150"
     CDS             3058..3912
                     /locus_tag="PLM83_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017689719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK71022.1"
                     /translation="MGSSTGRARRSTGGNVLVCLFLLINGVFMSLPLVYTVSNAFKPL
                     SELFIFPPRLWVRNPTFDNFRDLFVLMARSRMPFSRYLFNTIFISVAGTAGHVIMASL
                     AAYALSKHDFPGRQLMFNVVVLSLMFAPQVTAIPNYLIMSKLGWVDSHAAIIVPAWAS
                     SLGLYLMKQFMDQVPDSLLDAARIDGASEFRVFWQIVLPTVKPAWLTLIILSFQSLWG
                     ETGGRFIYSEQLKTLPYAMSQMVSGGIARAGVGAAVGLLMMIVPITVFLINQSNVVET
                     MSTSGMTG"
     gene            3937..>5241
                     /locus_tag="PLM83_06155"
     CDS             3937..>5241
                     /locus_tag="PLM83_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71023.1"
                     /translation="MIHKRFYTSLAVSVLMLLVYGSASAYAAPYSSYTYDWWKNAVPS
                     ASAYVPSIVVDGADMGVGPLKNPGDLYVRGDRIYVADSGNNRVVCTDRNFRPYMVISS
                     FHVPDGIDGFDEPKGVFVTEAGHIYVADKGNERIVEFDSEGEIVRIIGKPRSDVEGVL
                     SEDMIYRPLRVGVDRAGRVYVIADGIFDGIMEFGVDGGFKGFMGAPKVAPNPWEYFWS
                     KISTKEQKRRRALFLPIEYSSLDIDGRGFIYASVSGGATQKEEAVRRLNPTGVDVLRR
                     TGSFPPVGDVDFPHEESEASITGRSVLIDVAARENGMYSVLDRQRCRIFTYDENGELL
                     YIFGARGDVRGAFRNPVAIDWMGEAFLVLDEANGCITMLEPTEYASFMHQAIDLHNQG
                     KYDEAADMWRNVLRYNANCEAAYIGIGRSLMRQGLYSEAMDNF"
BASE COUNT         1219 a         1206 c         1528 g         1288 t
ORIGIN      
        1 cgccctcaag gcgaagcagg attacaagcg agggtcgttc accaaccgtc gcctctacgt
       61 aaatggcgat atcccgtttg ccgaggtgga cgcaataagg tttccatatg cgatcaacta
      121 cgagatgaga gtgcttgggg acgatgctgg ggagccgtat ctcttttatc ttcatgaagg
      181 ggtcaatgag ataaggcttg agccggtact gggcgatctc gggcccatgc tgcttcgggc
      241 agaggcagcc ctttacgaca tgaacacagt ttaccggaag atcgtgatga taacatcaac
      301 gaatccggat cctatgcgcg attaccagct ggaggaaaga gtgcccgaac tcatctcgaa
      361 attggctgaa ctgagcgagg tgatgcgctg cataggagag cagcttgagg catatacagg
      421 tgctcgcggg gactacgtgg cccttttgta tgagcttgcg cggcagttgg gcagtatggc
      481 gaaaaagcct gagagcattc caaagcgcat cgcagaactg cgtgatgccg gggcctctct
      541 tggatcgtgg attctttcca ctcgggagca gccactcatg atcgactaca tcgtcgttgc
      601 atcgattgaa caagagatgc caaaggcaaa accgacttgg ctccaaaggt tcaaacacga
      661 ggttaaggcc ttccttgcat cattcgtaca cagatacgat ttggtaggta atgtatattc
      721 aacagaaaat ggggacggcg tcagaccgct caaagtatgg ataggtgcag gacgcgatca
      781 ggctcaagca ctgaaatcca tgatagaaga ttctttcact cctgccacag ggatcccggt
      841 caatctcgaa cttgtctcga tgggggtgtt gctgccggca actctttcag ggaacggtcc
      901 agatgttgcc ctaggactcg atccagcaca gcccctcaat ttcgccctga ggggagctgt
      961 tgtcagcctg actcagtttc ctgactatcc tgaagttgcg gcaaggttca ggcccagtgc
     1021 gatgacgcct ttctctttcc gggatcaggt gtatgccttg cccgaacagc agccgttccc
     1081 tgtgatgttc tacagaaagg atattctgag ggagatgggc atttcgctcc ctgagacctg
     1141 ggacgatttg attggtatca ttcctgagct gcagaaatcg cacatggaca ttggtctgcc
     1201 agtttcaggc actggagcag taggagcggg aaacgttggc gaggcagtga atgcgggaag
     1261 cgtctcatca aatgctgggg taatgacttt ccttaccttg ctttatcaga acggcggacg
     1321 gctgtacggt cccgacggat tatgtacagg ccttgacgat gaggaagctg ttgacgcctt
     1381 tactaggtgg acagagctgc acgagctgta tactgttccc cttgactaca acgccatcaa
     1441 cagattcaga attggcgaaa tgccgatact tatcacgggc tacacactct acaacacgct
     1501 gtcggtcttt gcgccggagc tcagaggaga gtgggggttt acactggtcc cgggcacgag
     1561 acgcccggat ggaagcatag acagatcgtg tcctgcgggt agttgtggag ccgggggcgt
     1621 gccggggtta gcttcgtctg tggcctccgg gccagcaaca gtcataatga gctctgcgcc
     1681 tgacctagaa ggggcgtggg agttcgtaaa atggtggact agcaccgaaa cccaggtcag
     1741 gttcggacgt gagatggaga gcttgatggg gccgtcagca cgttatcctt cggcaaatgt
     1801 cgatgctgtc caggagcttc cttggaaatc cgatgagtgc cagatgctta tggagcagtg
     1861 ggagtgggta cgaggcatac cggaggttcc cggcggctac atggtgggca gacacctaga
     1921 taacgcattc agacgagttg tgtatcatca tgaaccccct cgtgaggttc tgctcgatta
     1981 caataggatg attaatgaag agataactat gaaacggttg gaattcgggt taccggtccg
     2041 ctacgacgag ctcgaggcag agtggtgcag tttcgattgg cagtgatggt gaaggagggg
     2101 taggacggtg accacagtag cgacagatgc tgagctgaga gaacctcatg gactgggatg
     2161 gaagataaag aagcacaggg tgtcttacct gttcgttgct ccttacgcta cattcttctt
     2221 cctgttcaca gtcttgccgg tggcggtagc gatagtgttg agtttcacag attacaacat
     2281 gttggagtcg cccaagtgga tagggtggga caattatctt cgcctgttct tggtggacga
     2341 tgtgtatcta atagctgtga agaacacctt catctttgcg gcgataacgg gtccgctcag
     2401 ttattttctg tgtttgatca tagcgtggtt ggtcaatgag ttgccgccaa aactgagggc
     2461 cgccttgacg ctgctgtttt acgccccgtc catatcaggc agccttttct tgatgtggac
     2521 gatcatcttc agtggtgatg catacggcat aataaacggc attctgctct attggggaat
     2581 cattgaccaa ccgattcaat ggctgaccga tccgaagtac atgatgactg tagtgattat
     2641 cgtcacctta tggatgagtc ttggaacaag ctttttggtg tttatcgccg gtcttcagaa
     2701 cgtagataag agtctatatg aagctgctgc cgtagacggg atcaagaata ggtggcagga
     2761 actgttcttc ataactcttc cggcaatgaa accccagctc atgttcggtg cagtcatgtc
     2821 catcacttcc tcgttcgcag cggcagggca gataattcct ttggtcggtt ttccaagcac
     2881 cgactatgcg gcccacacag tagtagcgca tctgctcgac tacgggaata ttcgatttga
     2941 aatgggatat gcttgtgcaa tcgcagtgat actctttgta actatggttg tggtgcagca
     3001 gattgtccag aggattctga ggaaccttgg atagggcagt cggggagggc atttgttatg
     3061 ggaagtagta ctggacgagc gagacgctca accggaggga acgtgctggt atgcctattt
     3121 ctcttaatca acggcgtctt catgtcgttg ccattggtct atacggtcag caacgcattc
     3181 aagcccctta gcgagctctt catattcccg ccacggctct gggtgcggaa tccgactttc
     3241 gacaattttc gggatctatt tgtactgatg gcgcgttcga ggatgccatt ttcccggtat
     3301 ttgtttaaca cgatcttcat aagcgttgcg ggaaccgccg ggcatgtgat catggcgtcc
     3361 ctcgccgcct atgcgctatc caaacatgat ttccccggaa ggcaactcat gttcaatgta
     3421 gtagtgcttt cgctgatgtt tgctccgcaa gttacggcga ttcccaacta cttgatcatg
     3481 tcgaagctgg ggtgggttga ctctcacgct gcgatcatag tcccggcatg ggcgtcatcg
     3541 ttaggactat acttgatgaa gcaattcatg gatcaggttc cagactcctt attggatgct
     3601 gcgaggatag atggagcaag cgaattcagg gtattctggc aaatcgtttt gcctacagtg
     3661 aagcctgcat ggcttacgct gatcatactc tcctttcaat cgctgtgggg tgagacagga
     3721 gggcggttca tttactcgga acagcttaag actttgccat atgccatgag ccagatggta
     3781 tcgggcggaa tcgccagagc cggggttggg gctgcggtgg gtttgctgat gatgatagtg
     3841 ccaataactg tgttcctgat aaaccagagc aacgtggtag agaccatgag cacgtcggga
     3901 atgacgggct aacccgctga aaggagaagc gtactgatga tccacaagag attctacaca
     3961 tcattggctg tatcggtgct aatgctgttg gtgtatggga gtgcttctgc ctatgcggcg
     4021 ccctacagta gctatacgta tgattggtgg aaaaacgctg ttccgtccgc atccgcctat
     4081 gttccctcaa tcgtggtaga cggggcggat atgggggtcg ggcccttgaa gaacccgggt
     4141 gacctttatg tgaggggcga ccggatctac gttgctgatt ccggcaacaa cagagtcgtc
     4201 tgcacggacc ggaacttccg cccgtacatg gtgatatcat cattccacgt cccggatggc
     4261 atcgatggct tcgacgaacc aaagggcgtt ttcgtgacgg aggcaggaca catttacgtg
     4321 gcagataagg gaaacgaaag aatagtcgag ttcgactctg aaggcgaaat cgtgaggatc
     4381 attggcaagc ctcggtctga tgtagagggt gtcttgtcag aagacatgat ctatagaccc
     4441 ttaagagtgg gcgtggacag ggcgggacgc gtctacgtca tagccgatgg cattttcgat
     4501 gggataatgg agttcggcgt agatgggggc ttcaaggggt tcatgggtgc tccgaaggtt
     4561 gccccgaatc cctgggaata cttctggtcg aagatttcca ccaaggagca gaagagaaga
     4621 cgagcattgt tcctgccaat agaatacagt agtctcgaca ttgacgggcg tggtttcata
     4681 tacgcctcag tctcgggagg cgctacgcag aaggaagagg cggttcggcg tctcaacccc
     4741 acaggcgtgg atgtgctgcg aaggacgggt tcttttccgc cggtaggcga tgttgatttt
     4801 ccacatgagg aatcggaagc aagcatcact gggcgttctg tgttgatcga tgttgccgcg
     4861 cgtgaaaatg ggatgtattc agtcctcgac aggcagcgat gccgcatctt tacatacgat
     4921 gaaaacggcg agcttctgta tatttttggg gctcgagggg atgtcagagg cgctttcaga
     4981 aaccctgtgg ccattgactg gatgggagag gcgtttctcg tcctagatga ggcaaacggg
     5041 tgcatcacaa tgcttgaacc cacagaatat gcttcattta tgcatcaagc tatcgacctg
     5101 cacaaccaag ggaagtacga tgaagctgcc gatatgtggc gaaacgttct cagatacaac
     5161 gcgaactgtg aagcggccta tataggcata ggcagatcgc tcatgcgtca aggtctatac
     5221 agtgaagcta tggacaactt c
//
LOCUS       DAOSAI010000152         5238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_9956_length_5238_cov_3.148370, whole genome shotgun sequence.
ACCESSION   DAOSAI010000152 DAOSAI010000000
VERSION     DAOSAI010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9956_length_5238_cov_3.148370"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLM83_06160"
     CDS             <1..302
                     /locus_tag="PLM83_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71024.1"
                     /translation="YLEKLRKEKGKTILIITHDMNIVSLYARRVVVMANGRVIGEGTP
                     REIFSQPQLMEQAYLRRPAIADLSDALWGEVCLTPQEAAYRLLESSRTNAEVSQK"
     gene            299..1132
                     /locus_tag="PLM83_06165"
     CDS             299..1132
                     /locus_tag="PLM83_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOK71025.1"
                     /translation="MIVEYESKQTSIHNLDPRAKFIWLIATIVISIIWTNPVYLAALG
                     LVTVVIGCMGEFPWAKMKGTFLFLLVITAIITLVQGATYVPKAANLAYPERVLFHLVP
                     GWIPGVNPAAPIRIGGFLYGVGMALKVFVVLIVISVFGYVTSPSEVVQIIVRIPFFSY
                     KSGFVVSAASKFVPVIQMQMATLRDAERSKGVDFDSGSFTQKVSKTTSMLIPLFANAL
                     SMADTMALAMESRAFGYSENFTLIRPYHMHWPDWLMAVLSIGALAASIVLLCVYKMGA
                     L"
     gene            1134..2165
                     /locus_tag="PLM83_06170"
     CDS             1134..2165
                     /locus_tag="PLM83_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOK71026.1"
                     /translation="MGPTDGVVVSLGAAAMDMVFSVSKLPEPDEMVFAEGEVCFFPGG
                     STANIAAGIGRLGYRSRFVGRVGNDDNGRRLRRAFEADGVDVRWLAEIEGGRTAQTII
                     AVDRRGERVIYSLGGEAILQEPDEFEDVAMEGAEILYIGEVLPDVAARAIQLARLYGV
                     TVVYGPGGGISWIDSATLYQLLKECDYVLLSRGELDAVTSCPSHTEGAGRLLESGVRN
                     VIVTLGSDGSECHSRGAGRSPWYGSSWNGRAGDVAHDMVNGSSNGRGTNTVLRAESFR
                     VTNVVDTTGAGDSFAAGFITGLLRGFDIVECLERGNACAALAIQKTGAREAMPTSEEL
                     EEFLRYAER"
     gene            2152..3180
                     /locus_tag="PLM83_06175"
     CDS             2152..3180
                     /locus_tag="PLM83_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOK71027.1"
                     /translation="MQRGSLDPSRVAGTLAGVAIGDAMGMPSEFMTREEIRDVYGRIE
                     GFCVPHEGHIHAGMAGGCITDDTEQTIAIIEALERHDRITPEVAAAAYLRWAQQCNAY
                     ESSVLGPSSRRALERLKAGEEPRVTGSSGNTVGAAMRVAPIGIVNSPNLESAAEECYM
                     SCLPTHGVNIAIAGASAICCGVAAALVAESLDEVIDATVFGARHGERLGIEWAGTLVS
                     ARIELALRIVRESRDAVEAEDQLYTTCGVGMDPTELVPTAIGLFALHEGDPQRTVPAA
                     ANMGGDTDTLASMAGALSGAYRGIGAFPEEWVRTVEEVNNLNFEELTVILVAVRERRR
                     VNNGWNRC"
     gene            3161..3919
                     /locus_tag="PLM83_06180"
     CDS             3161..3919
                     /locus_tag="PLM83_06180"
                     /inference="COORDINATES: protein motif:HMM:NF005556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HOK71028.1"
                     /translation="MAGIDVRLRRIFGEDGKAVICPLDFGGFMGPVKGLYDPSAAVRK
                     VVEGGCDAILVNPGLAATEWESFAGKTGLVVRVTGGSTKFSPNPAFHTLVCSVEEACR
                     LGADAICIMLLVGSDYEQEMFEIMGQVVSEAHVLGIPVLAEVIPCDPAHSFDPEWISV
                     CARVGYELGADAIKTYCTAEGFEEIVRACRVPIVIAGGPKVEDPNSVVRRAMSAGAAG
                     IAFGRNVFQADDPVAKVKELYSIVHGDCSCKEGR"
     gene            3922..4962
                     /locus_tag="PLM83_06185"
     CDS             3922..4962
                     /locus_tag="PLM83_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOK71029.1"
                     /translation="MKAVVYHGPGDLRVENVDDPVLEEGDVLLGVSYCGLCGTDVKTY
                     KRGHHMFTPPCILGHEVVGRIVEIVGEPVKPDIAVGDMVAVAPYVPCYGCAYCRSGRE
                     ELCTNKGFIEGAFAEYVRIPAEVVSKGTFHIPSEEDIKLACLAEPLACCVNAAQDSHV
                     RLADTVMIIGAGPMGLLMLELCKAMGASKILVSEPNGFRRNKAAERGAVVIDPACSGT
                     DVSAWAKEQTDGIGADVVFVCVGATEAVDAALGCVRKGGTVNIFGGLPSGSRLTVDPH
                     ILHYDEVILTGSFGFTPLQFHTALTMLTGGAIDAGSIITHEFDVDQAVEAFDMASSGE
                     SLKILLKVDGDA"
     gene            4955..>5238
                     /locus_tag="PLM83_06190"
     CDS             4955..>5238
                     /locus_tag="PLM83_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOK71030.1"
                     /translation="MHDFSLMGIDIGTSAVKVLLWRSDGTHRAAYRRAKESYVTGRGV
                     GVAASEQDPAALWRIVTQCIREALQGWDAGSVAGVGLSGHGPSLMAINRGG"
BASE COUNT         1124 a         1228 c         1713 g         1173 t
ORIGIN      
        1 actacctgga gaagctccgt aaggagaagg gcaagaccat actgataatc acccacgaca
       61 tgaacatagt cagtctgtat gccaggcgag ttgtggtcat ggcgaatgga cgtgtgatcg
      121 gtgaaggaac tccgcgtgag atcttctcac agccccaact gatggaacaa gcgtatttgc
      181 gaaggcctgc gattgccgac ttgtctgatg ccctgtgggg cgaggtgtgc ctgaccccgc
      241 aagaggcggc gtataggctc ttagaatcat caaggacgaa tgcagaggtg tcccaaaagt
      301 gattgtcgag tacgaaagca agcaaacgtc tatacacaat ctcgatccca gagccaagtt
      361 catctggttg attgccacga tagtcataag catcatctgg accaacccgg tctatttggc
      421 tgcactgggg ttggtgactg tcgtcattgg ctgtatggga gaattcccct gggcgaagat
      481 gaaagggacc tttttgttcc tgctcgtcat cacggcaata atcacgctgg ttcagggggc
      541 tacgtatgta cccaaagctg ccaaccttgc ttacccggag cgggtgttgt tccacttggt
      601 gccaggctgg attcctgggg ttaacccggc agccccgata cggattggcg gattcctcta
      661 tggagtcggg atggcgctca aagtattcgt ggtccttatt gtaatatcgg tatttggata
      721 tgtaacatcg ccgtcggaag tagtgcagat cattgtgcgc atcccgtttt tttcctacaa
      781 atctggattc gtggtctcag cggcctcaaa gttcgtgccg gtgatccaga tgcagatggc
      841 caccttgagg gacgcggaga gatcgaaagg cgttgacttc gattcaggat ctttcaccca
      901 gaaggtgagc aagaccacta gcatgctgat tccgctgttc gcgaacgcgc tttctatggc
      961 tgatacgatg gcattagcca tggaatctcg ggcatttgga tacagcgaga acttcacatt
     1021 gatcaggccg taccacatgc attggcctga ttggttgatg gctgtcttgt caatcggggc
     1081 cttagcggca agcatagtac tgctttgcgt gtacaaaatg ggggcgctct agaatgggcc
     1141 caacggatgg tgttgtagtc tctctcggcg ctgctgcgat ggacatggtg ttttccgtga
     1201 gtaagctgcc cgagccggat gaaatggtat ttgctgaagg agaggtctgc tttttcccag
     1261 gagggtcaac tgcgaatatc gcagcaggaa tcggtcggct aggctatcgg tctaggttcg
     1321 tgggcagggt tggtaatgac gacaatggcc gtagattgcg gcgggctttt gaagctgatg
     1381 gtgtggatgt gaggtggctt gcggagatcg aggggggtag gaccgcccag accataattg
     1441 ctgtggatag aaggggcgaa agggtcatct attcccttgg cggcgaggct attttgcagg
     1501 aaccggatga gtttgaggac gtggccatgg agggtgcgga gatcctttac atcggtgagg
     1561 tgttgccgga cgtagctgct cgggccatcc aacttgcgag gttgtatggc gtcactgtcg
     1621 tctatggccc cggaggcggg atctcatgga tcgacagcgc gacactctat cagctgctga
     1681 aggaatgtga ctatgttctg ctctcccgcg gagagctcga tgccgtcaca tcgtgtccct
     1741 cgcataccga gggtgcaggg cggctgcttg agagcggcgt ccgcaacgtc attgttaccc
     1801 tgggctccga tggatcggaa tgccactccc gcggggctgg acgaagtcct tggtatggca
     1861 gctcttggaa tggtcgggca ggcgacgtag cccatgacat ggtgaacggc tcgagcaatg
     1921 gtagaggtac caacactgta ttgcgcgcag agtccttccg tgttaccaat gttgtcgaca
     1981 caactggggc cggtgattcc tttgccgcgg gattcatcac aggtctgctg cggggctttg
     2041 atatagtcga gtgccttgag cggggcaatg cctgcgcagc gctggccatc cagaaaactg
     2101 gggccaggga ggcgatgccc actagtgaag aattggagga gttcctgcgt tatgcagaga
     2161 ggtagcttgg atccaagcag agtagcaggc acccttgcgg gagttgccat aggggacgca
     2221 atgggaatgc cgtcggagtt catgacgcgt gaagagattc gcgatgtata tgggaggata
     2281 gagggattct gcgttcccca tgaaggacac atccacgccg gcatggccgg aggttgcatc
     2341 accgacgaca cggaacagac gatcgcgatc atcgaggccc tggaaaggca cgataggata
     2401 actccggagg ttgctgccgc cgcatatttg cgatgggccc agcagtgtaa cgcctacgaa
     2461 tcatcggtgc tagggcctag ttcgcgcaga gctttggaga ggcttaaggc gggtgaagaa
     2521 cctcgggtga cggggtcatc cgggaacacc gtcggagctg ctatgcgcgt tgcgccaatt
     2581 gggatagtca attcccccaa cttggaaagt gcggctgaag agtgctacat gtcgtgcctg
     2641 ccgacgcacg gtgtgaacat agctattgca ggggcgtctg caatctgctg cggtgtagcg
     2701 gctgctcttg ttgcagagtc gctggacgag gtgatcgatg ccaccgtgtt tggcgcccgg
     2761 catggggaac gcctcggaat cgagtgggcg ggtacactgg tgagcgctcg tattgaactc
     2821 gcactcagga ttgtgcgcga gagcagggat gcggtagagg cggaggacca gctttacacg
     2881 acgtgcgggg ttggaatgga tcccacagag ctggtgccga cggccatagg tctcttcgct
     2941 ctgcacgaag gcgatccgca gaggactgtg ccagcggccg caaacatggg aggcgacaca
     3001 gatactcttg catcgatggc gggggccttg tcaggcgctt accgcggcat tggggcgttc
     3061 ccggaagaat gggtgcgtac tgtcgaagaa gtcaacaacc tgaattttga agaactcacg
     3121 gtgattcttg tggctgtaag ggaaaggaga cgcgtcaata atggctggaa tagatgttag
     3181 gcttcggcgg atattcggag aggacgggaa ggcggtcata tgcccgctcg attttggagg
     3241 tttcatggga ccggtcaagg ggctgtatga tccctctgcg gcagtcagaa aagtggtcga
     3301 aggagggtgc gacgccatac tggtcaatcc gggcttagcc gccacggaat gggagagctt
     3361 cgcagggaag acgggccttg tggtcagggt gacgggaggc tccaccaaat tcagtccgaa
     3421 ccccgcattc cacacgctgg tctgctcggt ggaggaagca tgtcggcttg gcgccgacgc
     3481 catctgcatc atgcttttgg ttggttccga ttatgagcag gaaatgttcg aaatcatggg
     3541 ccaggtagtc tctgaggcac acgttctcgg gattcccgtt cttgccgagg ttatcccgtg
     3601 cgatccggcg cacagtttcg atccagaatg gataagcgtg tgcgcacgag tcggatacga
     3661 actgggcgcg gatgcgataa agacatactg cacagccgag ggctttgagg agatagtcag
     3721 ggcgtgcaga gtcccgattg tcatagcagg cggtcctaag gtagaggatc ccaatagtgt
     3781 agttcgccgc gccatgtcag ctggggctgc gggaatagct tttggtcgca acgtgttcca
     3841 ggctgatgat ccggtagcaa aggtcaaaga gctatacagc attgtccacg gagattgctc
     3901 ctgcaaggag ggacgatagc ggtgaaagca gttgtgtacc acggccctgg cgatcttcgt
     3961 gtggagaacg tagatgatcc ggttctcgag gagggcgacg tgctgcttgg ggtctcctat
     4021 tgcgggctct gcgggacgga tgtcaagaca tacaagcgcg ggcaccacat gtttacccct
     4081 ccgtgcatcc tcggccatga agtggtcgga agaatagtgg agatcgtagg cgagcctgta
     4141 aagccagaca ttgccgttgg cgatatggta gcagttgccc cgtatgtccc atgctacggc
     4201 tgcgcctatt gccgcagtgg cagagaggaa ctttgcacta acaagggctt tatcgagggg
     4261 gcctttgcgg agtacgtaag gattcctgcc gaagtcgtca gcaaggggac gttccacatt
     4321 ccgtcagaag aagacatcaa actggcgtgc cttgctgaac cacttgcctg ctgtgtgaat
     4381 gctgcgcagg actctcatgt gaggctggcg gatacggtca tgataattgg ggcagggcca
     4441 atgggcctcc taatgctcga gctgtgtaag gcaatgggag cttcgaagat tctggtgagc
     4501 gagcccaatg gtttcagaag gaataaggct gctgaaagag gggcggtggt tatagatcct
     4561 gcatgttcag gaacggacgt ttcggcatgg gcaaaggaac agacggacgg gatcggtgct
     4621 gacgtggtat ttgtctgcgt tggagctact gaggcagtgg atgcggctct tggatgtgtc
     4681 agaaagggag gaacagtcaa cattttcggc ggactgccaa gtgggtctcg gctaactgta
     4741 gatccgcaca tccttcatta tgacgaagtc attcttaccg ggagtttcgg cttcacgccc
     4801 ctgcagtttc acacggcatt gaccatgctg acaggaggcg cgatagatgc ggggagcatc
     4861 atcacgcacg aattcgatgt ggatcaggcg gtagaagctt ttgatatggc gtcctcaggg
     4921 gaatctttga aaatactcct gaaggttgat ggcgatgcat gacttttctc tcatggggat
     4981 agacatagga acctcagcag tcaaggtgtt gctgtggcgt tcagacggca cccatcgggc
     5041 ggcataccgt cgcgccaagg aatcatatgt aacgggccgg ggtgtgggcg tggctgcctc
     5101 tgaacaggat cctgctgcgc tgtggcgcat tgtgactcag tgcatacgag aggcgttgca
     5161 gggatgggat gcgggcagcg tggccggcgt gggtctgtca ggacatgggc cgtcgcttat
     5221 ggcgatcaac agaggcgg
//
LOCUS       DAOSAI010000153         5204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10033_length_5204_cov_3.275005, whole genome shotgun sequence.
ACCESSION   DAOSAI010000153 DAOSAI010000000
VERSION     DAOSAI010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10033_length_5204_cov_3.275005"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..335)
                     /locus_tag="PLM83_06195"
     CDS             complement(24..335)
                     /locus_tag="PLM83_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOK71031.1"
                     /translation="MKLKIGGKIVLSYTIVLLVMAAISILGIQADRKITNEYMNLIER
                     SLPVEATIYRLRSELLEKGLALRGYILSGNPAEIERFHLLDVNVQQSMDKARMLSQAG
                     K"
     gene            520..2730
                     /locus_tag="PLM83_06200"
     CDS             520..2730
                     /locus_tag="PLM83_06200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOK71032.1"
                     /translation="MGKINVSSLRNRFALVLIIVLLPWIVVNVRNNLAEQEELMRDAE
                     ERSLKLLDYLDETERLNLRSIMRIIETALLGRDGQDGGHVDLMKVQMLVDVFGCELPV
                     AITDFGGNVLASSFPLDNEELVRENPQVRAAADTGKISFGSFQGILGPDKYGVVVAYP
                     VIDPSYGIRSIIVGFVDEERLADPLRGLELPADAVFEVADLDGNVVARYSRAKEDMRR
                     GEAMEQAMLKSGAAEISERSRSGFMKLEDGSMQLVAEKIIVDPCGRPTLRVAVTTPAA
                     SSLSRMNDHILRDLKALLVVAAIITPVLWGIFRAEVVVPVEKLANLASRLAQGEFGIR
                     HGEPYRRGELGLLARTFDQMSTALERRANELIYLSHHDSLTGVYNRTYIESIEEDLNA
                     EDKLPTTVIVGDINGLKRINDTYGHSVGNEVVIAAGNIFRQCVGEKGIVARWAGGEFI
                     AILPNTDWKEGLAVCREISHSCSQMEPDPVAPNIALGVATRATLEQSLRDTWIAAEDR
                     MYTNKLAGSTSGRGRLLHSLRQALAESTHETQQHSSRLHFLATELGKVMGLSAGALDD
                     LALLADFHDIGKIGIPDAILQKPGPLTPPEWDMMMAHPEIGARIVGGCYELEHIADAI
                     LSHHERWDGTGYPNGLAGDEIPLISRILAIVDAYDAMTSDRPYRTAMSREEALEEIQR
                     CSGTQFDPNLVEVFVKMMEEIDLKEEYEKNWFEGDPYWLCTTFDESKLIGDGDS"
     gene            2862..2937
                     /locus_tag="PLM83_06205"
     tRNA            2862..2937
                     /locus_tag="PLM83_06205"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2896..2898,aa:Glu,seq:ttc)
     gene            3565..>5204
                     /locus_tag="PLM83_06210"
     CDS             3565..>5204
                     /locus_tag="PLM83_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOK71033.1"
                     /translation="MKVNTSRLQCRMMLLILLMAVPLLAASVQNGFSQRKAMVQQIEM
                     QGLDLLGYMESAMSMGRKRNIETIAMVVLGEGEDFDIEAKQRAIDKIVAKTGNRLDIA
                     IIDDRGRILASSTTQFDSALLADKERLQEALLTGQISFGVHKPDHAGGRPGVLLLYPV
                     FDADNSATFVVAALLYEELLFSSLSNVDLPADVAFELVDLNWDVVAHRETSDRALGLT
                     ASPDLGDVRQKLAFSVDGGLSEVSKWEDGVKRLAGIKTVTDYRGNPVFYVTATMPIGA
                     DLTRATILSFYQVAVMLGVSLIVIPVTWGLMNKQFVEPVNRLVDLAEDLGSGHMDARH
                     GEPYEAGEVGELAKAFDEMAEALKERSEELEYLSFHDSLTGAYNRTYIESISEELACE
                     ENLPTTIILGDINGLKMVNDTYGHQAGNELIVRAGAIIQQCVGDSGVVARWGGDEFII
                     VLPNAPMRSGLLLCNRIRSACESSGHYPMAPSIALGVATRMAAEQSIMDTLVSAESRM
                     YRNKFLDTSSARGTLLYSLRRALAESTHETEEHSSRMC"
BASE COUNT         1262 a         1197 c         1481 g         1264 t
ORIGIN      
        1 gccaacaact ccaacgactg caattacttg ccagcctggc ttagcatgcg ggccttatcc
       61 atgctctgct gaacgttcac atcgagcaga tggaatcttt cgatttctgc gggattgccc
      121 gagagtatgt aaccgcgcag ggccagccct ttttccaaaa gctcgctgcg aagcctgtat
      181 attgtggctt ctacgggcaa gctccgctcg atcaggttca tatattcgtt cgttatctta
      241 cgatcagctt gaatgccgag tatgcttatg gcagccatga ctaaaaggac gattgtgtac
      301 gacaggacga tcttcccacc tattttgagc ttcattggcc tacctcctgt tgtgtcatgt
      361 aataatctta tcggcaaacg caagcaccga tattagatac gacatcaaaa tgttcgatat
      421 aatttgatat agactgacac ggtttatgcc gatatagata atgggggcat tggcatcgct
      481 catctaccaa acctgcggta aggtggggag ataccaaacg tggggaagat taatgtttca
      541 agtctgcgca accgtttcgc gcttgttctc ataattgtcc ttttgccgtg gattgtagtg
      601 aatgtcagaa acaatctagc tgaacaagag gaattgatga gggatgcaga agagcgaagc
      661 ctgaagcttc ttgactatct cgacgaaaca gagcgtctca atctgaggag catcatgagg
      721 atcatcgaaa ccgcgctgct gggcagagat gggcaggatg ggggccacgt tgaccttatg
      781 aaggttcaga tgcttgtcga tgtatttgga tgcgaattgc cggtagcaat aacggatttt
      841 ggaggcaatg tgttggcgtc ctcttttccc ttggacaatg aagagcttgt gcgtgaaaac
      901 ccgcaggttc gcgccgccgc cgatacaggc aagatctcgt tcggcagctt ccaagggatt
      961 ctcggccctg ataagtatgg ggtcgtagtg gcctatcccg tgattgatcc gagctatggc
     1021 atccgctcaa tcatagtagg tttcgtagat gaagaaagat tggccgatcc cttgcgtggt
     1081 ctcgagctac cggcagacgc tgttttcgag gtggctgacc tggacgggaa cgtggtcgcg
     1141 cgctattctc gtgcaaaaga ggatatgagg cgtggcgaag ctatggaaca agcgatgctt
     1201 aaatccgggg cagcggaaat cagcgaaaga agccgcagtg ggttcatgaa gttggaggat
     1261 ggctcaatgc agctcgttgc agaaaagata atagttgatc cctgcggcag gccaacgctg
     1321 cgcgtcgccg ttacaactcc ggctgcctct tctctttctc gcatgaatga ccacatcttg
     1381 cgcgacctga aagcgctttt ggtggtagct gcaatcatca cccctgttct gtggggcata
     1441 ttccgcgcag aagtggtggt gcctgtagaa aaattggcga acttagccag tcggctagca
     1501 caaggagagt tcggaatcag gcacggcgag ccttaccgcc gtggggaact tgggcttctt
     1561 gcgaggactt ttgaccagat gtcaacagca ttggagagac gcgcaaatga actcatctat
     1621 ctcagccatc acgattcgtt gaccggtgtc tataaccgaa cctacatcga aagcatcgaa
     1681 gaggatttga acgctgagga caagcttcct actacagtga ttgtgggcga cataaacggg
     1741 ttaaagagga taaacgatac ttacggacat tcagtcggca atgaggttgt gattgccgct
     1801 ggaaacatct ttcgccaatg tgttggagag aagggaatcg tagctaggtg ggcagggggc
     1861 gagttcattg ccattttacc aaacaccgac tggaaagagg gcctggcagt gtgcagggag
     1921 atcagtcatt cctgcagcca gatggagcct gatccagtag cgcccaacat agccttgggg
     1981 gttgccacac gcgccacact cgagcagagt ttgagagaca cgtggattgc ggctgaggac
     2041 cggatgtaca caaacaagct tgcaggatca acatctggca gaggaaggtt gctccattcg
     2101 ctgaggcaag cattggctga aagcacacat gagacgcagc agcattcaag cagattgcat
     2161 tttctcgcca ctgaactggg gaaagttatg gggctttctg caggtgccct cgatgatcta
     2221 gcgctgctcg cggacttcca cgacataggg aagatcggga ttccagatgc tatacttcag
     2281 aagcccggtc cgctcacgcc tccggagtgg gacatgatga tggcgcaccc tgagataggg
     2341 gcgaggattg ttggtggatg ctacgaacta gagcatatcg cagacgccat cttatcccat
     2401 cacgaaaggt gggatggcac gggatatcct aatgggcttg cgggggatga aattcctctg
     2461 atctcgcgga tccttgcaat tgttgatgcc tatgatgcca tgaccagtga ccgtccttat
     2521 cgtacagcga tgagcaggga agaggcgctt gaggagatac agaggtgttc gggaacgcag
     2581 ttcgacccga atttagttga ggtatttgtg aagatgatgg aagagatcga cttgaaggag
     2641 gaatacgaga agaactggtt tgaaggagac ccatactggc tgtgcacaac gtttgacgag
     2701 tccaagctta ttggcgacgg tgattcttga tcagatccta ttgctttgct gtgttacctt
     2761 atccggcagt gtgacgtcgg tttgacgaat tgctgttcac gtgatatcat agctgagcaa
     2821 aagacttatg cgttaagtta agatttgcca tatatgtttg cgctcccatg gtctagtggc
     2881 ctaggacatc gccctttcaa ggcggtaaca cgggttcgaa tcccgttggg agcgccatta
     2941 ttatgccctt ttttcatctc tcctcggcag tatgaggaat gtcggatcat cctcgggctt
     3001 ctcctctgcc ttttccctgt gttcaagcgg ggcaacctgc tactgagccg ttgtgacgcc
     3061 atatgaccct cttatcggca gattcactca aacactgtca attgttgatt gcggcgtgca
     3121 cagcaccgcc agggagatta cgcttcgttc aaccttgttc tgggacaggc cgctgactat
     3181 agggaacgag tctggttggc atcggcgctt tcatcgcaaa gaatatctgc tccattccgg
     3241 cgtttgcgcg tttatgtgca caagagcgat tccgccagaa gatctcatgt gacaggaaga
     3301 gctcgtgcac ggcatgtgca ggttttgctt ctgcgggcag aaacagtcaa cgaaagtatt
     3361 tgatagagtt cgcaagtgta gtataataag ttctggaaag atacacattt tttatttgac
     3421 atcagtgggt agaagctctc cgaggttggc tcaacggatt gccgatatgt gccgatatat
     3481 acaaatgggc ccacaaattg taccgaatgc gtcgattacc cttcttgagc atggccaatg
     3541 atctggaagg agaagaaacc tgctatgaag gtgaatacgt cgcgtctgca atgtaggatg
     3601 atgctgttga tcctgttgat ggctgtgcca ttgcttgcag ccagtgtcca aaatggcttt
     3661 tcccagagga aggcaatggt tcagcagata gaaatgcaag gccttgatct gctaggatat
     3721 atggagtcag cgatgagcat gggccggaag cggaacatcg agacgatagc gatggttgtg
     3781 ttaggggaag gggaagactt cgatatcgag gccaaacagc gagccatcga caagatcgtt
     3841 gctaagacag gaaacaggtt ggacatagcc attattgacg accgaggcag gattcttgct
     3901 tcctccacaa ctcagttcga ctccgcatta ctcgcggata aggagcgcct tcaggaagcg
     3961 ctccttacag gtcagatctc cttcggtgtg cataagccgg accatgcagg cggcaggccg
     4021 ggggtgcttt tgttatatcc agtcttcgac gccgataata gtgctacttt tgttgttgca
     4081 gcgcttcttt atgaagaact gcttttttct tccctgagca acgttgatct tcccgctgat
     4141 gtagctttcg aactcgtcga cctcaattgg gatgttgtag cgcatcgcga gacatctgat
     4201 agggctttag gccttactgc aagcccagac ctgggcgatg tcaggcagaa actggcattc
     4261 agtgtagatg gaggcttgag cgaggttagc aaatgggagg acggggtcaa gcgcctagcg
     4321 ggcatcaaga cagttacaga ctatcgcggg aatcctgttt tctatgttac cgccactatg
     4381 ccgattgggg cagatctgac ccgcgcgacg attttgtcct tctatcaagt tgcggtgatg
     4441 cttggagtat cgttgatcgt tattcctgta acatggggat tgatgaacaa gcagtttgtg
     4501 gaaccagtca acagactggt cgacttggca gaagatcttg gaagcggcca catggatgcg
     4561 aggcacggcg aaccctacga ggcgggcgag gttggcgagc ttgcgaaggc tttcgatgag
     4621 atggcggagg cgcttaagga acgcagtgag gagctggagt atctcagttt ccacgacagt
     4681 ttgaccggag cctataaccg cacttacatc gaaagcatca gcgaggaatt ggcttgtgag
     4741 gagaaccttc cgacaacgat aatattgggc gatatcaatg gcctgaagat ggtgaacgat
     4801 acctatggcc atcaggccgg aaacgagcta atagtgaggg ctggcgccat catccagcag
     4861 tgcgtcgggg atagcggcgt tgttgccaga tggggcggtg acgaattcat aatagtcttg
     4921 cccaacgcgc ccatgcgaag cggcctcttg ctgtgtaaca ggatacgttc ggcttgcgag
     4981 tcgtccggac attatccgat ggcgccaagc atagctttgg gtgttgccac ccggatggct
     5041 gccgaacagt cgataatgga cacgcttgtg agcgccgaaa gccgtatgta caggaataaa
     5101 ttcctcgaca cgtcatcagc caggggcact ctgctttact cgctccgaag ggctctcgcg
     5161 gaaagcaccc atgagacgga agagcactcc agcagaatgt gctt
//
LOCUS       DAOSAI010000154         5197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10045_length_5197_cov_3.963244, whole genome shotgun sequence.
ACCESSION   DAOSAI010000154 DAOSAI010000000
VERSION     DAOSAI010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10045_length_5197_cov_3.963244"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2241)
                     /locus_tag="PLM83_06215"
     CDS             complement(<1..2241)
                     /locus_tag="PLM83_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOK71034.1"
                     /translation="MKAMLLGAGEGTRLRPVTATRPKPMIPIANRPIMEHILLLLKSH
                     GVRDVYSNLYYLADQIESYFGDGSALGLSLKFKVEEKLPGTAGGVKNLEEHFDETFCV
                     ISGDLLTDFDLTRAHEFHRSRGAIATILLTHIDNPLEYGVVITDKDGRIQRFLEKPGW
                     AEVFSDTINTGIYILEPAVFDFIPPGVDFDFSRDLFPLLLREGQPLFGYVADGYWCDV
                     GNIDMYLKAQVDALSGKVKLNIPGEQITPGIWVGRSAKISSRADLKGPMVLGENCEIA
                     PGARLREFTVIGDNVIVAPNSFVVRSTVFSNSYIGENSIVSGSIICKNVVLKDSVRVG
                     EGAVISDDSILNRSVTVKPGIRVWPGKSIDESVVLSSSVVWEQRLRRELFHSGKISGL
                     VNFEFAPEFALRLGNAIGGTMKKGESVIVSRDPSRTARMMKRALAAGLSSSGVTVCDL
                     QGVPAPILSHMVKRGDFAYAIHMQTSDRNYELVDINVFDSEGLALSREAERKLETLLA
                     REDPRRTSAREVGGIGYYPRAIDIYTEDCLSRIDVETTKANRMKLVVDCGYGFGGSIL
                     PSMLVRMGVEVTSLNAIEQETKMPKSAEQFVESLDELALIVKTVDADMGIAIDPQGTK
                     MWVTDDTGRVLTDMELAYLFSLLTVRSRDSRNVAMPSYMPSVFTQSLTANGATVYRAR
                     ANARSLADLARTSKSSISSDLVGGFIFPNLHNGFDAIFGAAKFTELVARDAAPLSQLA
                     QLVPEHH"
     gene            2880..>3435
                     /locus_tag="PLM83_06220"
     CDS             2880..>3435
                     /locus_tag="PLM83_06220"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HOK71035.1"
                     /translation="MRDVQNFPDLRGINIQKVGVKDVHLPLQIAAREGGCQSVLGIVS
                     LSVALPMQFKGTHMSRFMEVLLPWSRKPISNVEILAILKETCEALSAQRADISIRFKY
                     FVPRIAPVSGKESILDYNCSFEGTLDGKRHSFAMGVEVPVTSCCPCSKEISEYGAHNQ
                     RAMIRARVKFSSAGFLWLEDLIELL"
     assembly_gap    3436..3445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3446..3673
                     /locus_tag="PLM83_06225"
     CDS             <3446..3673
                     /locus_tag="PLM83_06225"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HOK71036.1"
                     /translation="EGSCEIYPLLKREDEKYVTEAAYDNPKFVEDILRDVVIRLRNEP
                     RVRWFEVECESMESIHNHSAFACHVEDKRMV"
     gene            3674..>5197
                     /locus_tag="PLM83_06230"
     CDS             3674..>5197
                     /locus_tag="PLM83_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOK71037.1"
                     /translation="MSVLEIDSLKKSLGASPIFEDVCLSVAEGDHIGLVGRNGSGKST
                     LLKIIAGIEDYDSGSVALASGCEAGYLAQGKEYTSGNSLYEEMKRAFAKTFEIGAAMR
                     ELESRMSDPEIIASERELDRVMRRYSRLTADYELLGGYDYDVRIKSTLFGLGFVEEDL
                     ERIIDTLSGGQKVRVALARMLVSAPNLLLLDEPTNHLDVNACEWLEDYLAAFNGAFIV
                     ISHDRYFLDATVDRIWDIDDLKVVEYPGNYTFFAAEKELARQRQAQEYQRQQETIQKL
                     EAYIRRYKAGNRSTMAQSREKMLARIERIQKPREDSQMKLSFGKAARSGRLAVSVSGV
                     SKSYGDKTLFEAVDLAVERGERLGVVGPNGSGKTTFLRIIADDLAPDSGSVDFGENVT
                     LGIFWQDLAGLDDTLTVLDELYRARDWTLGEARSYAARFLFRGEDVFKPVKALSGGER
                     NRLILAKLILNGPNLLLLDEPTNHLDIESRHALEEAIAEFDGTVICASHDRYFLDQTA
                     "
BASE COUNT         1171 a         1256 c         1491 g         1269 t
ORIGIN      
        1 gtgatgttcg ggaacgagct gcgccagttg ggagagggga gcggcatctc gtgccaccag
       61 ttctgtaaac tttgcggcgc caaatatggc gtcaaagccg ttgtgcaggt tgggaaagat
      121 gaagccgcca accaggtcgg atgatatgga cgacttactc gtgcgtgcta ggtcggcaag
      181 gcttcttgca ttagccctag cccggtatac cgttgcgcca ttggcggtaa gcgattgtgt
      241 aaatactgac ggcatatagc tgggcatggc aacgttccgt gaatccctcg agcgcactgt
      301 tagaagtgag aagagatagg ctagctccat gtcggtcaat acgcgtcctg tgtcatcggt
      361 tacccacatc ttcgttccct gcgggtctat ggcaatgccc atgtcggcat cgaccgtttt
      421 gactattagg gcaagttcat cgagggattc tacgaattgt tcggcggact ttggcatctt
      481 agtctcctgc tcaatggcat tgagcgatgt gacttccacg cccatcctaa ccaacatcga
      541 tgggaggata cttcctccaa aaccataacc gcagtctact acgagtttca tccggttggc
      601 ttttgtcgtt tccacgtcga tacgggagag acaatcctcg gtgtagatat cgattgctct
      661 gggatagtag cctatgccgc ccacttcacg ggcgctcgtg cggcgagggt cttcacgcgc
      721 caacaaggtt tcgagcttgc gctctgcttc gcgcgacagt gccaggcctt ccgaatcgaa
      781 cacattgata tccaccagct cgtagtttct gtctgaggtc tgcatgtgta ttgcataggc
      841 aaaatctccg cgtttgacca tgtgagaaag gataggcgcc ggcacaccct gcaagtcgca
      901 tactgttact cctgagctcg ataggccggc ggcaagggcg cgtttcatca tcctcgctgt
      961 tcggctggga tcacggctta ctatgacgct ttcacccttc ttcatcgttc ccccaatcgc
     1021 gttgcccagt ctcagcgcga attcaggagc gaattcgaag ttgaccagcc cggatatttt
     1081 gcccgaatgg aatagctcgc gcctcagacg ttgctcccac actacgctcg atgacagcac
     1141 cacgctctca tctatgctct tgccgggcca tactcgaatg ccgggtttta ctgtgacgct
     1201 gcggtttaga atggaatcgt cggagattac tgcgccttcc ccaacgcgga cgctatcttt
     1261 gagcaccaca ttcttgcaga ttatggagcc cgacacgatc gagttttcgc ctatgtagga
     1321 gttgctaaag accgtcgacc ttacaacaaa ggagttgggg gccacgatta cgttatcacc
     1381 gatgacggtg aattcgcgca gccttgcgcc aggggctatt tcgcagtttt cccccagaac
     1441 catgggacct ttgaggtcgg ccctggaact tattttggcg gatcggccca cccagatacc
     1501 gggtgtaatc tgctcgcccg ggatattaag cttgactttg ccggaaagcg cgtctacttg
     1561 ggctttgaga tacatgtcaa tgttgccgac atcgcaccag tagccgtcag ccacgtatcc
     1621 gaagagcggt tggccctccc ggagcagaag ggggaagaga tcacgcgaga agtcgaagtc
     1681 cacgcctgga gggatgaaat cgaagactgc gggttcaagt atgtagatcc ctgtgttgat
     1741 tgtgtcggag aatacttcag cccaccccgg cttttccagg aaacgctgta tgcgtccatc
     1801 tttatccgtt atgaccacac cgtattcaag agggttatcg atgtgtgtga gaagaatcgt
     1861 ggcgatggcc ccgcggcttc tgtgaaattc gtgcgcccgt gtgagatcga aatctgtcag
     1921 cagatcgcct gaaatgacgc agaaggtttc atcgaagtgt tcctcgaggt ttttgactcc
     1981 gccggcggtg cctggaagtt tttcttcgac cttgaacttg agggacaggc ctagtgcaga
     2041 tccgtcaccg aagtatgatt ctatctgatc ggcaaggtag tagaggttgg aatatacgtc
     2101 gcggaccccg tgagatttca acagcaggag tatatgctcc atgatcggtc gattcgctat
     2161 ggggatcata ggtttcgggc gtgttgccgt gactggtctc agtcgggtac cttctccggc
     2221 gccaaggagc atggccttca taaccccacc cccgtattga tatgataatg ctgccttttt
     2281 gctccatact tggcagctcg attgatcaat aatgggtttc gacgaggacg agcgaagtcc
     2341 ttcagacacg actgatgggt ttgcggtagg gggatttgca cagaaactga gcagaattcg
     2401 gtcagcggct cctgcagcca ggtagttggg tttcgattgg cggtcgactt cagttcgtcc
     2461 aggcacgatc tgatgcgctc tcagatagtc tttgcgtgat catcgagctt gtgccatctt
     2521 ctgtgcttcc cggtcggctt acggcagttc tctaacgaat gccatttggg aatttcaagc
     2581 tccatcgtta tcgactttta caaactttcg ccactgaaac gagcatttcg gcatttaaga
     2641 agaaagcgat gaattactgt gaactgcaat cgccacgcat gtccccagag gctcggacta
     2701 ttggctcaac accaaatgcc cggggtgaac cgaattgagt atggattttg cggctccaat
     2761 cggctagtgt ttgtagtagg tcgtgctgat gcgctcgata gaaggtataa tgagcgcttt
     2821 cgtagaaaaa ggagcagtta ggcaatggga aaaggcgcga tatgagagga ggcgccggaa
     2881 tgcgggatgt acagaacttt ccggatttac ggggcatcaa catacagaag gttggtgtaa
     2941 aggatgtgca tctgcctctg cagatcgctg cgagagaggg cggctgccag tcggtgctgg
     3001 gaattgtgtc gctatctgtg gcacttccaa tgcagtttaa ggggactcac atgagcagat
     3061 tcatggaagt ccttctgccg tggagcagga aacccatatc aaatgtggag atactcgcta
     3121 tcctcaagga gacgtgcgag gccctttcag cgcaacgggc ggacatatcc ataaggttca
     3181 agtattttgt tccaaggatt gcgccagtca gtggcaaaga gagcatcctc gattacaact
     3241 gcagttttga gggaactttg gacggcaaac gccactcgtt tgctatgggc gtcgaggttc
     3301 cggtcacatc atgttgccca tgcagcaagg agatttccga atacggcgcc cacaaccagc
     3361 gtgcaatgat tcgggctcgg gtgaagttta gttctgccgg gtttctgtgg cttgaggact
     3421 tgatcgagct cctagnnnnn nnnnngaggg aagctgtgag atttaccccc tcctgaagcg
     3481 cgaggatgag aaatacgtga ctgaggctgc ctatgacaac cccaaattcg tggaggacat
     3541 acttcgggat gttgttatac ggctgcggaa tgagccgcga gtgagatggt tcgaggtaga
     3601 gtgcgaaagc atggagtcga tccataacca ttcggcattt gcgtgtcacg tcgaagacaa
     3661 gaggatggtg taggtgtcgg ttcttgaaat agattccttg aagaaatctc ttggtgcttc
     3721 tcccatattc gaagatgtct gcctgtctgt cgccgaaggc gatcacattg gccttgttgg
     3781 acggaacggg agcggcaaga gcacgcttct gaagatcatc gccggaatcg aggattacga
     3841 ttcgggctct gtggctcttg cttccggctg cgaggcgggg tacctcgctc aaggtaaaga
     3901 atacacatca ggaaacagcc tttatgaaga gatgaaaagg gcctttgcga agacgttcga
     3961 gataggggcc gcgatgcggg agctggaaag ccggatgtcc gatcccgaga ttattgcgtc
     4021 agagcgcgag cttgacaggg taatgcgacg gtactcccgg ctcactgccg attacgagct
     4081 tcttggaggc tatgattatg atgttcgcat caagtctacc cttttcgggt tggggtttgt
     4141 cgaagaggac cttgagagaa tcatcgacac tctcagtgga ggccagaagg tgcgcgtggc
     4201 tcttgcgcgc atgctggttt ctgcgcccaa cttgcttctg ctcgatgaac ccacgaacca
     4261 cttggatgtg aatgcatgtg aatggcttga ggattacctt gcggccttta atggcgcttt
     4321 catagtaatc tcccatgata ggtactttct cgatgccacg gtggatcgca tatgggatat
     4381 agatgatctg aaggttgtgg aatatccggg gaattacacg ttcttcgcgg ctgagaagga
     4441 gctagcacgg cagaggcagg cccaggagta ccagcggcaa caggagacga tacagaagct
     4501 ggaggcgtac attaggcggt acaaggcggg aaaccgcagc acaatggcgc aaagtcgcga
     4561 gaagatgctt gccagaatag aacgcatcca gaagcccagg gaagattcac aaatgaaact
     4621 gtcgtttggc aaggcggcgc gctccggccg tttggctgtt tctgttagcg gtgtatcgaa
     4681 gagttatgga gataagacac tttttgaagc tgtagatctt gcagtggaac gcggggagag
     4741 gttgggtgtt gttggaccta atggctctgg gaagacgaca tttctgcgaa taatcgcaga
     4801 tgatctggct cctgattccg gctcagtgga ctttggcgaa aacgtgactt tgggcatatt
     4861 ctggcaagac cttgcaggcc tcgatgatac actgactgtc cttgacgaac tgtaccgcgc
     4921 ccgagattgg acattgggtg aggccaggag ttacgcagcg cgcttccttt ttcgagggga
     4981 ggacgtgttc aagcccgtga aggcactttc gggcggggaa cgcaatcgcc ttatccttgc
     5041 caagcttatc ttgaatgggc ctaatctgtt gttgctcgac gaacccacta accacctcga
     5101 catagagtca cgccacgcat tggaagaggc tattgccgaa ttcgatggga ctgtgatatg
     5161 tgcaagccat gatagatact tccttgacca gacagct
//
LOCUS       DAOSAI010000155         5175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10090_length_5175_cov_3.070898, whole genome shotgun sequence.
ACCESSION   DAOSAI010000155 DAOSAI010000000
VERSION     DAOSAI010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10090_length_5175_cov_3.070898"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="PLM83_06235"
     CDS             complement(<1..1194)
                     /locus_tag="PLM83_06235"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOK71038.1"
                     /translation="MARPSRKNGEYERALPPTLLALGMAAVLIVVGAVGGAIWMQLRV
                     PPQLCAVLAEAGGGSGTQFGENDVAALVRYVQKCSSAGFPDVPLTRIQKIIGERSSVA
                     AVEANQEVEAELAREAHEAVLRQNGGEWGSKFHRLRIDRIVDGLSHVAGDDASGYKIA
                     MLDTPQINAFSTADGRIYLTRGLVTTSSDDEIALVIAHEMHHIRSGHWINWWALSAGD
                     GSFDADTENPQEGGAMAEAALALVKMGTKAFSGEASTSYEQEYEADAMAILHAAECGY
                     CVRRIFRILTRLPEMPVTSHPTSSDRIEGASEVLESMGDPSWIAVHNPAEVARLALEN
                     AIAALRGDRPGICAQVVGLDEVASICARTLKTLEGVEDEMRAYNWLGHGWPYADDSRM
                     VVRTAL"
     gene            complement(1306..1797)
                     /locus_tag="PLM83_06240"
     CDS             complement(1306..1797)
                     /locus_tag="PLM83_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK71039.1"
                     /translation="MEAIDAIRGRRSVRKYRPDPVPREIIEEIVDCGRLAPSARNDQP
                     WEFVVVTEQSIREEIAAVATYGKFIADAPVCLVAFSRQTPRFVEDTCAAFQNMLVAAY
                     AHGLGTCWIAGHNTPYEKEIARICKAPGDYRLVALSPLGYPDEECTVHKRELQEVLHW
                     ESF"
     gene            complement(1923..2858)
                     /locus_tag="PLM83_06245"
     CDS             complement(1923..2858)
                     /locus_tag="PLM83_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOK71040.1"
                     /translation="MKNLWIVKDEVTDALNQERPVLAFESTVLTHGLPYPVSRDLALR
                     VESIAREEGCVPATMGIIDGVVHVGMTPEEIDRLAADGAASRAVKASGHDIAFVVASG
                     MSAGVTVSGTLAIAERAGVRVFATGGIGGVHRGASETFDISHDLVSIARSPVVTVCAG
                     AKAILDLPKTMEYLETVGVCVLGYRTDRLPAFYSADSGIPLSWRVESADEVADAFLAR
                     EELGIHGGLLVAAPIPVEKELSSEEIDAAIEDAVALADERGVRGKALTPYLLARLAET
                     TGGRSVEANLGLLENNARVGAEIARAISCKLRERG"
     gene            3219..3443
                     /locus_tag="PLM83_06250"
     CDS             3219..3443
                     /locus_tag="PLM83_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71041.1"
                     /translation="MKTKPLLATVLAGLLLTAGALAYAKTTPPKTADAEAEGLTIEGS
                     GSLQAPAAKLVAEVKILKAVDDLGLSKEQV"
     gene            3447..>3656
                     /locus_tag="PLM83_06255"
     CDS             3447..>3656
                     /locus_tag="PLM83_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71042.1"
                     /translation="MAKLAYNLDDEVTKGIDAIVKQLEIEKNELLAGPAGDAVHGAES
                     KIADEKAAIGTAVSDAQKEAVKILSP"
     assembly_gap    3657..3666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4270..>5175
                     /locus_tag="PLM83_06260"
     CDS             4270..>5175
                     /locus_tag="PLM83_06260"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOK71043.1"
                     /translation="MLYIPNDSNDPRFNLAFEEYVLKNLDMDDPYLLLWRNAPTVVVG
                     RFQNTPDEINADYVEEHGVHVVRRMTGGGAVYHDLGNLNFSFIERKTSEGIDMKLFAR
                     RIAQALQGVGLDVEVSGRNDLTIDGRKFSGNAQYIYHDRVLHHGTILYSSDLDNVQAA
                     LNVKADKIASKGVKSVKSRVTNLIDYMPVPLSIEEFQKSLLLQLFQGAPVRTRVLTEA
                     ELEEINELVAAKYAKWEWNFGRSPAYNVSNRGRFGCGEVAVYLWVDEGIIRSCRIHGD
                     FFTARDIHDLESGLTGIECSKETLSK"
BASE COUNT         1207 a         1631 c         1265 g         1062 t
ORIGIN      
        1 aagggcggtg cgaaccacca tcctgctgtc gtcggcatac ggccacccat gacccagcca
       61 gttgtaggcc ctcatctcat cctccacgcc ttccagtgtc ttcaaagtcc ttgcacaaat
      121 tgaagccacc tcgtctaagc ccacaacttg cgcacatatt cccggtctat cccctcgcaa
      181 ggcggcaatg gcgttttcca aagcaaggcg tgcaacctca gccgggttgt gcaccgcaat
      241 ccatgaagga tcccccatag actcgagaac ctcgctcgcc ccctctatcc tgtcagacga
      301 agtaggatgg ctcgtgacag gcatctcggg cagacgcgtc aggatccgga atatccttcg
      361 cacgcaatag ccgcactcgg ccgcatgcaa tatcgccatg gcgtcagctt cgtattcctg
      421 ctcatacgat gtggacgcct cacctgaaaa cgcctttgtc cccatcttta ccaaggccag
      481 tgcagcctcg gccatcgcgc cgccttcctg cgggttttcc gtgtcagcat caaagctgcc
      541 gtctcccgca ctcagcgccc accagttgat ccagtggcct gaacgtatgt gatgcatttc
      601 gtgggcgatg acgagggcaa tctcgtcatc cgaagacgta gtgaccaagc ctcgggtaag
      661 gtatatcctc ccgtccgcgg tagaaaaggc attgatctgt ggagtgtcga gcatggcgat
      721 tttgtatccc gaagcatcat cacccgccac atgagacagt ccgtcgacta ttctatctat
      781 tctgagccga tggaactttg agccccattc tcccccgttt tgccggagaa ccgcctcgtg
      841 agcctccctt gccaattcgg cctcgacctc ctggtttgcc tctacagccg ccacgctgga
      901 acgctcgcct ataatctttt ggatccgtgt gagcgggaca tccggaaagc ccgccgacga
      961 gcatttctgt acatagcgca ccagcgcagc cacatcgttc tcgccaaact gagtcccgct
     1021 gcctccgccc gcctcggcga gcacagcgca aagctgtgga ggaacccgaa gctgcatcca
     1081 tattgcgccg cccactgcac ccacaactat tagaaccgcg gccatgccca gcgccaacag
     1141 tgtcggcggc aacgcccttt catattcccc gtttttccta gaaggccttg ccactgccga
     1201 tatttccgct caacctccaa cttcgcgtcc gccccgcaaa ggacagtcga tgaattgctc
     1261 ctggacaact atggaacggg ccaaaagagg ctaatctaga tccccctaga agctctccca
     1321 gtgcagcacc tcttgaagct cgcgcttgtg aaccgtgcat tcctcatccg ggtagccaag
     1381 gggagagagt gccaccagac ggtagtctcc aggggccttg catatgcgcg ctatctcctt
     1441 ctcatacggc gtgttgtgcc ccgcaatcca gcacgtgccc aaaccatggg cgtaggcggc
     1501 aaccagcatg ttctgaaagg ccgcgcacgt atcctccaca aagcgtggag tctggcgaga
     1561 aaatgccaca agacatacag gggcatcggc tatgaacttc ccgtatgtag ctacagctgc
     1621 tatctcttcc ctgatcgact gctcagtcac tacaacgaat tcccatggct gatcattccg
     1681 ggcactcggc gccaacctgc cgcaatccac aatctcctct atgatctctc ggggcacagg
     1741 atcaggccga tacttgcgca cgcttcgcct tccccggatt gcatctatag cttccatttt
     1801 atctcctccc gcaaatagtc tcgtgcaggc caaacgtgcc gggttcacgg cacacacttc
     1861 ccacacaaca gggcctcggc ccgcctgcaa tgtcccactt cttggagtcg aagtgccgca
     1921 agctatcccc gctcgcggag cttgcacgat atagccctgg ctatctccgc tccaacccgg
     1981 gcattgttct cgagcaatcc caggtttgcc tcgaccgacc ttcccccggt cgtctccgca
     2041 agccgggcaa gaaggtaagg cgtaagggcc ttcccacgca caccccgctc atcggcgagc
     2101 gccacggcat cttcaatagc cgcatcgatc tcttcggagg aaagctcctt ctccaccgga
     2161 ataggagctg ccaccaagag cccgccatgg attcccaatt cctctctagc caaaaaagca
     2221 tccgcaactt cgtcggcgga ctcgactcgc caagagagcg gaatcccaga atcggcagag
     2281 tagaacgccg gaagtctatc agttctgtat cccagcacgc acactcccac agtctccagg
     2341 tattccatgg ttttgggcag atcaagtatc gccttcgcgc ctgcgcacac agtaaccacg
     2401 ggcgaacggg caatgctgac aagatcgtgg gaaatgtcga aggtctcgga cgcgccccga
     2461 tggacgcctc caattccgcc agttgcaaac actcgtaccc cggcgcgctc ggcgatggca
     2521 agcgtgccgg agacggtgac ccctgcagac atcccagaag caacgacaaa tgctatgtcg
     2581 tgtccagatg ccttgacagc cctagaggca gcaccgtcag ccgccaggcg gtctatctct
     2641 tccggtgtca ttccaacatg cacaacgccg tctataatac ccatagttgc aggaacacac
     2701 ccctcctcgc gggcaatcga ctccacccgc aaggccaagt ccctgctcac aggataagga
     2761 agcccatgag tcagaacagt cgactcgaaa gccaacacag gcctctcctg attgagcgca
     2821 tctgttactt catccttgac aatccataag tttttcataa ccctgccccc atgcaaccga
     2881 tttgtgcggc agtggatgcc gcagtctgac actggcccca ttcccacctt taggcgcttc
     2941 aacacgtttc gcaaggttcc ttctcaacag gaactcataa acttcgcgga cccgttcttt
     3001 gcctgctact cccctcatca cttgcaccag gcaacttcca agccgtcccc cacaacaggc
     3061 gcccgcggaa agtcttatcg gccctgaccg tccctgtctt tccaagccct gcccgagttg
     3121 gactgatgag catcgcaacc catgcattcc cctgaactat gccgcccatc tttcgtttat
     3181 cattactgac gatactactt tctcagggag gttgcaccat gaaaaccaaa ccactcctcg
     3241 ccaccgtcct ggccgggcta ttgctcacag caggcgcact agcgtacgca aaaaccactc
     3301 cgcccaagac tgcagacgct gaagcggagg gactaacaat cgaaggctcc gggtcactcc
     3361 aggcacctgc agcgaaactc gttgcggaag tcaagatcct caaggccgta gatgatctag
     3421 gcctttcgaa ggaacaggtg tagtccttgg ccaaactcgc ctacaaccta gacgacgagg
     3481 ttaccaaggg catagatgca atcgtcaaac agctggaaat cgagaagaac gaactgctgg
     3541 caggtcctgc gggggacgcg gttcacggag ccgaaagcaa gatcgcagac gaaaaggctg
     3601 ccataggcac agcggtttca gatgctcaga aggaagctgt aaagatactc tcccccnnnn
     3661 nnnnnncatg gcagacgggc caaagatgtt gaaggcccta tccgatctgc tcgatcagaa
     3721 actggctact atgtagacgg attccacaac gcatacgacc ccggccgggc gccgatttca
     3781 ggccttctta ggcttgaatc gggcccggca cgactccctc actccgctct agcctgagct
     3841 cgggcttatc cggtatggaa tacttgtccc atgaaatctt cgttattgaa tctatatggt
     3901 atgatcccaa tttccgatct gggtagactt gtaaccatag ctgctaaaga ggcattttgt
     3961 cccgcactct gactcgagct atgcggtctg cgaaatgctc gtggtattcg agtaatcagt
     4021 atctttggcc ccctcattcg cgcgtgcgcc tcaacaaaag cctcacgcca accttccgca
     4081 gacctgagat tggcactttc cgaagtccat agtatcatta gacttgcaac gccgaggcct
     4141 gcacgcgtcg cgctccgcac cctacgtttc gcgtcctgcg ctctcggcag catataacgt
     4201 gccaagctcg taggcgaaca aacacagtcg agcggtcggc agacaggcaa gctgcttagg
     4261 aggatagcta tgctctacat accaaacgat tcgaatgatc cccgattcaa cctggcattt
     4321 gaagaatacg tactgaaaaa cctcgatatg gacgatccct accttctcct ctggcgcaat
     4381 gcccctacag ttgtcgtagg ccgctttcag aacacgcccg atgaaatcaa tgccgactac
     4441 gtagaggagc acggagtgca cgtagtgagg cgaatgactg gcggcggagc ggtctaccac
     4501 gatctgggca acctcaactt cagtttcatc gagcgcaaga cctcagaggg aatagacatg
     4561 aaactgtttg cccgcaggat agcccaggcc cttcagggag tcggcctgga tgtggaagta
     4621 agcgggagaa acgatctgac cattgacgga cggaagttct ccgggaacgc ccagtatatc
     4681 taccacgaca gagtgctcca ccacggaaca atcctataca gctcagacct ggataacgtg
     4741 caggcagccc tcaacgtcaa agcagacaag atcgcctcta aaggcgtcaa gtcagtcaaa
     4801 agcagggtaa caaatctcat agattacatg cccgttcccc tttcgatcga ggagtttcag
     4861 aaatcccttc tgctccagtt gttccagggc gcccctgttc gaacgcgagt cctgacagag
     4921 gcggagctag aggagataaa cgagctcgtg gcggcaaaat atgccaaatg ggagtggaac
     4981 ttcgggagat cgcccgccta caacgtaagc aacagaggaa gatttggctg tggggaggta
     5041 gccgtatacc tttgggttga tgaagggata atccgatctt gcaggattca cggagatttc
     5101 tttaccgcaa gagacataca tgacctcgag tcggggctga cggggatcga atgctctaaa
     5161 gaaaccctta gcaag
//
LOCUS       DAOSAI010000156         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10248_length_5107_cov_3.830166, whole genome shotgun sequence.
ACCESSION   DAOSAI010000156 DAOSAI010000000
VERSION     DAOSAI010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10248_length_5107_cov_3.830166"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    82..346
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            438..1886
                     /locus_tag="PLM83_06265"
     CDS             438..1886
                     /locus_tag="PLM83_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOK71044.1"
                     /translation="MANVRFFTDQHLPLEMHKVRIVQKLNLPPIERRLEAITEAGNNT
                     FLLQNRDIFLDMLTDSGVNAMSDQQLAAMMIADDSYAGSETFTKLENKVIELFGMPHF
                     LPAHQGRACEHIIASTLVKPGNVVPMNFHFTTSKAHIVRCGGIVRELLIDEGLKPTSE
                     FPFKGNVDLNKLRQVIEEEGAGNIPFVRLEAGTNLIGGQPLSLENVREVAAVCHSNGI
                     PVVLDASLLQDNLYFIHEREEACKDMSIREITRAIADEVDVIYFSARKLGFAKGGGIC
                     IRDRVMFEKMRELVPLFEGFLTYGGMSVREMEAIVVGLDETMDEDVISQGPQFIQFMT
                     DELRRAGVPVVTPPGGLGCHLNAMEFLPHVEQGQYPAGALASALYIAGGVRGMERGTM
                     SEAREPDGREIFSQMELLRLAMPRRVFTLSQVKYAIDRIIWLYEHRDLIGGLTFVEEP
                     AILRFFFGKLKPTSDWQTDLAKAFREDFGDSL"
     gene            complement(2246..2776)
                     /locus_tag="PLM83_06270"
     CDS             complement(2246..2776)
                     /locus_tag="PLM83_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71045.1"
                     /translation="MKSRSTLLVSCALSLVLLLGLATSASANSRTFIGAKYKGAVGDL
                     ALAYPGINAGLELEYRFDNGFAIAAACTAGFLKAEVDDGPTVDIGLYSAALGARYYVM
                     LGDFNPYFGISGTALIANAMGASATLPGLQATAGLEMTFNKIRFAGEVGYEAFIIDKK
                     LEEGGIVYGVSLGYCF"
     gene            3366..4073
                     /locus_tag="PLM83_06275"
     CDS             3366..4073
                     /locus_tag="PLM83_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71046.1"
                     /translation="MAVARSDLAFDTPVAMKSSGVAATGSDRPGYSYYFAYCAYALLA
                     MVMTGVSSIMMSFNRPDLYLRNLCAPLPGTSMSLQLAAGHAVFALGCWAILVVGSFVL
                     HGESLVSSGLAGLYSLNTLAFAAVSAFIGFLVGSSVKSNGAQAGAINVITLGMSFLCG
                     VFVPQSIMSKSVLSVGRFLPTYWFIRANDAIGEISSFTADSLRPIYGSILIQLGFAVA
                     VFSVTLLLSKERRLSHL"
     gene            4250..5092
                     /locus_tag="PLM83_06280"
     CDS             4250..5092
                     /locus_tag="PLM83_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HOK71047.1"
                     /translation="MRTEQEMMGLILGVAAADERVRAVVMNGSRADPNAPRDIFQDYD
                     IVYYVTDIDSYAEDRSWVDIFGERMIMQMPKSDCCLVYLMQFTDGNRIDLTLVPLFEK
                     QEFSTKDKLAVALMDKDNVLPPLPPPTDEDYWVRPPSRQDYDECCNEFWWVAVYVAKG
                     LCRREILYAKWRLDRIVREMLLQMLEWEASIATDFLVSMGKCRKRLEAHLPEEQWQAL
                     LLTYADGSYEGTWNSLFAACDLFRQTAKFVASHFGYEYPGGDDQRVGKHLEWLRDLCA
                     SSGV"
BASE COUNT         1161 a         1263 c         1516 g         1167 t
ORIGIN      
        1 gaatttgttc acgaatggct gaaattgatt cttggcaagg cgggtcggct ttgatataat
       61 aacagccaat aagcatacag cacaaagacg atgaccgaga agaagtagga catgacgtag
      121 gcccgacaga gagttcccgg ctggtgtgag gggcggggtt gttatgtctg aatacatctt
      181 ggtagtcgct cacccaaagg cgcattgacg cccggtaggc tgagacggat tcacccgtta
      241 cagtgataga gtatcgcgca ctattagcgc cgtacttgaa aaggccggca acgcgagttg
      301 tcggcaaatt gaggtggtac cacgggattt ggcgctcgtc ctcttgctaa ggcaggggac
      361 gggcgcttct tgctttcccc gcaatatggg cgtgctggcg ccaaagggca ctcccaagtt
      421 aaaggagtgt atttttgatg gcaaacgtaa ggttcttcac agatcagcac ttgcccctgg
      481 agatgcacaa ggtacgtatc gtacagaagc tgaatcttcc tcctatcgag cgcaggctag
      541 aggctatcac cgaggcagga aacaacacat ttcttcttca gaatcgggac atctttctgg
      601 atatgctcac agacagcggg gtcaatgcca tgagcgatca gcagttggcg gcgatgatga
      661 tagctgacga cagctatgca ggctctgaaa ctttcaccaa gttggaaaac aaggtgatcg
      721 agcttttcgg catgcctcac tttctccctg cccatcaggg acgtgcctgt gagcatatta
      781 ttgcgtcaac tttggttaag cccggcaatg tcgtgccgat gaacttccac tttactacca
      841 gcaaagcgca tattgttaga tgtggcggca tcgttcgcga gctactgatc gacgagggcc
      901 tcaagcctac aagcgagttt ccgttcaagg gaaacgttga cctgaacaag ctgaggcagg
      961 taattgagga ggagggagca ggcaacatac catttgttcg gctggaggcg ggcacaaact
     1021 tgattggcgg gcagcctctc tcgttggaga atgtccgcga ggttgcggca gtctgtcact
     1081 ccaacggcat cccagtcgta ctcgatgcca gccttcttca ggacaacttg tatttcatcc
     1141 acgagcgtga ggaagcatgt aaagatatga gcatacgaga gatcacccgt gcaatcgccg
     1201 acgaggtgga tgtgatctac ttctccgcgc gcaaacttgg ctttgcaaag ggcggaggga
     1261 tttgtatccg cgaccgtgtc atgttcgaga agatgcggga gttggtaccc ctcttcgaag
     1321 gtttcttgac atacggcggc atgtcagtgc gggagatgga ggcgattgtg gtcggacttg
     1381 acgaaacaat ggatgaggat gtaatctccc agggaccaca gttcatccag ttcatgaccg
     1441 atgagcttcg cagggccggc gtgccggttg tcacccctcc tggagggctg ggatgccatc
     1501 taaatgcaat ggaatttctg cctcatgtag agcaaggcca gtatcccgcc ggcgcactgg
     1561 cgtccgcgct ctacattgct ggtggggtgc gaggaatgga gcgtggaacc atgagcgagg
     1621 cgcgcgagcc tgatggcaga gagatttttt ctcagatgga actgctgcgt cttgcgatgc
     1681 cgcgacgcgt cttcacactt tctcaggtca aatatgcgat cgaccgcatc atctggctct
     1741 acgaacaccg ggatctgatc ggggggttga cctttgttga agaaccggcg atcctccgct
     1801 ttttcttcgg caagctgaaa cccacttcgg attggcaaac tgatctggcg aaggcttttc
     1861 gagaggactt cggggacagc ttgtagtgga ttggatattt ccacatatcc tggggccaag
     1921 acgggttcgg gatacccgga tgtgcacgtc ccaaggctgt taggaatcga ttggactcca
     1981 ccgcggggga aggcttggtt aacagccgaa ctcttgcagg ccacggcagg gaaggggcgg
     2041 gttctgtgcc ttgaaactag aggatgtggc gcctgagctg aagtgggcgt acatgcatcg
     2101 atcaatctgg gggcgtgaac catgcggagt tcgcgccccc cagggtaggt tcagctgcta
     2161 caagatctcg gctgctcaac tctgcactgc acgtagagac cgattatcgg cggttggttt
     2221 tcgaatatgt cagcagacgg ggagactaga aacagtatcc aaggctgacg ccgtatacta
     2281 ttccgccttc ctccagcttc ttatcgatta tgaaggcttc atatccgacc tcgcctgcaa
     2341 agcgtatctt gttgaacgtc atctccagac cagcagtggc ctgtagacca gggagtgttg
     2401 ccgaagcgcc cattgcattg gcgatgaggg cggttccact gatcccaaag taagggttga
     2461 aatctccgag catgacataa tacctggctc cgagagcggc actgtacaga ccaatgtcga
     2521 cggtaggccc gtcatctact tccgccttaa ggaaaccagc ggtgcatgcg gcagcgatcg
     2581 caaacccgtt atcgaacctg tattccagct cgagaccagc attgatgcca ggataagcta
     2641 aggcaaggtc gccaacggct cccttgtact ttgcgccgat aaaggtgcgg ctgtttgccg
     2701 acgctgaagt ggccaatcca agaagaagga ccagtgacag ggcacatgac accagtagtg
     2761 tgctgcgaga cttcacttgc atgacctccc aaataggtag ttggtaggat tgtctaggtt
     2821 ccaagaatcc aacaagctct tgccctgggc tgatgcagac cgactgctgg tctgtccgct
     2881 agccctgcgt caatactaca ttctgcaatc acctccagaa ttcacgaatt atggatgaaa
     2941 cagaagcagt atatgtatgc caatgcaatt cggcaaaagg gttcccgaac ctcccacaca
     3001 tgcggcctat aaatcgccag atgtgtgcca gacattccca gaaaccatgt atagctggcg
     3061 aacgtcggaa tacgtcaaat agcgcgataa ttggggcagc aggattggcg gtgtgatagc
     3121 tcaaaagact cgtctggagg tccaccgggt gaagggcttg gattctgagc tgattggaaa
     3181 aacgccaccg gattcattct ctcgtgtggt gcggaggggt ggcccgagac ttcatacgaa
     3241 ctaggcaggg cgattctgca actgcagaga gcaaagaagc gtgaattcaa tgaaaggcaa
     3301 ttgagtgtct cattgagtgg aagacgaaga aaatacggtg acggacaatt gttagacggt
     3361 ccttggtggc agtcgcccgg tccgacctgg catttgacac tccggtcgcc atgaaatcct
     3421 ctggcgtggc agcaaccggg agcgatcgtc cgggatactc ctactacttt gcatactgtg
     3481 cctatgcgct gcttgccatg gtcatgacgg gcgtaagctc catcatgatg tcgttcaata
     3541 ggcccgatct ctacttgcgc aacttgtgcg cgccattgcc gggcacgagc atgagcctgc
     3601 agttggctgc cggacacgcg gtgtttgccc tgggttgctg ggccatactc gtggtaggca
     3661 gcttcgtgct gcacggcgaa agcttggtgt cgtcggggtt agcagggctg tattccctca
     3721 acacgttggc attcgcagcg gtgagtgcgt tcataggttt tctcgtgggc agctccgtga
     3781 agagtaatgg cgcacaagcg ggagcgatca acgtcatcac tcttggcatg agcttcctgt
     3841 gtggtgtgtt cgtacctcag tcaatcatga gcaagtcggt gctgtcggtc ggaaggtttt
     3901 tgccaacgta ctggttcatc cgggcgaacg atgccatagg ggagatttcg agcttcactg
     3961 cggatagcct gcgtcccatc tatggcagca tcctgatcca gctggggttc gctgtggccg
     4021 tattctcggt gacgttgctt ctgagcaaag agcgaaggct gtcgcatctc tagggccaag
     4081 ccgcctacgc aatgcaggtt ccaagagact ggacgagcca acgccgcccg gattatcgtg
     4141 acgcgggtgg cggcatgttg agacatcagt ggctgcctga ttgtgctaag atgcttttgg
     4201 agcatcacac atgttgacgc ttcggacgca atgcttggga ggaactcaga tgagaactga
     4261 gcaggagatg atgggcctga ttctcggcgt agcggcggcc gatgagcgcg tgcgcgcggt
     4321 ggtcatgaat ggttcgcgcg cagaccccaa cgctccccgg gatatcttcc aagactacga
     4381 tattgtctac tacgtgacag acatagattc atatgcagaa gacagaagct gggtcgacat
     4441 cttcggcgag cgaatgataa tgcagatgcc caagtccgat tgctgccttg tctatctcat
     4501 gcagttcaca gacggaaaca ggatcgatct cactttggtg cctctgttcg aaaagcagga
     4561 attcagcaca aaggacaagc ttgcagtggc cttgatggac aaggacaatg ttcttccacc
     4621 attgcctccg cccacagatg aggattactg ggtacgaccg ccgtcccgtc aggactatga
     4681 tgagtgctgc aacgagttct ggtgggtggc tgtttatgtg gcgaaggggc tgtgcaggcg
     4741 ggagattctt tatgccaaat ggcgcctgga tagaatcgta agggagatgc tccttcagat
     4801 gttggagtgg gaggccagca tagcgactga cttcttggtg agcatgggca agtgcaggaa
     4861 gcgcctcgag gcgcatttac cagaggaaca atggcaggct ctgttgctca cgtatgcaga
     4921 cggaagctat gagggcacat ggaattcgct gtttgcagcg tgcgatctgt ttcgtcagac
     4981 ggcgaagttc gtggcttcgc actttggcta tgaatatccg ggcggcgacg atcaacgcgt
     5041 cggcaagcac ttggagtggc tacgggatct ctgcgcgtct tccggcgtgt agggaccgct
     5101 cttctgg
//
LOCUS       DAOSAI010000157         5093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10302_length_5093_cov_3.642715, whole genome shotgun sequence.
ACCESSION   DAOSAI010000157 DAOSAI010000000
VERSION     DAOSAI010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10302_length_5093_cov_3.642715"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2650
                     /locus_tag="PLM83_06285"
     CDS             <1..2650
                     /locus_tag="PLM83_06285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOK71048.1"
                     /translation="MRVQDLVNRQLVQLGRKLGVPVVATNDVHYLRREDATAHDVLLC
                     IQTGSVVDDPSRMRFPTDQFYLKAAEEMYREFAEIPEACAMTARIAERCNVELDFDTL
                     HMPEFEAPPGQTLDSYLRRLCEEGAIRRFGAISREVRERLDYELGIIERMGYSGYFLI
                     VWDFIRYAKSQGIYVGPGRGSAPGSLAAYCLGITDVDPLAYNLLFERFLNPERVTMPD
                     IDVDFCYERRGEVIDYVAGKYGSDKVAQIITFGTMAARAAIRDVGRALKFSYAEVDRI
                     AKLVPAELNMTIDRALEVSPELAAAVGESERNRTLIEIARAVEGLPRHPSVHAAGVVI
                     SKDPLTDHVPLYKSADGVLTTQYPMEDLERLGIVKMDFLGLRTLTVIGNTVEIVKHTR
                     NEDVDIESIPLDDPMVYEMLRNAESEGVFQLESSLFQNMLREVKPTKFEDLVAIVALG
                     RPGPMVMTGDFVRGKHCHDTVKYPHPALEKILEPTYGVMLYQEQVMQAASELAGFSLG
                     EADMLRRAMGKKKPEVIAGLRDRFMEGALGRGVSERAAQEVFSLIERFAGYGFNKSHS
                     AAYALISYRTAYLRCHYFPEFMASTLTSVMGSSERVAMYIDVCRAAGVDVLPPDVNES
                     FKGFTVSGNTIRFGLGAIKNVGEGAAESIISARKSGGPFVSFVDFCNRVDMTAVNRKA
                     LESLIRCGAFSEFGKRRALLAIMDQVCDQSAVRQRHQESGQASFFDLFEEPSGFGTAD
                     IPLPDVPDFSESQILAMEKELLGLYISGHPLASVAEAIRKVATMSVRDLSNAEDGAYA
                     TLAGVIIGRKQIIARTGQPMAFVQIEDLTGQVEVVVFPRTYQECADILARDAIVVVKG
                     RVDVKEEGIKILADSITELPKILANA"
     gene            2803..3006
                     /locus_tag="PLM83_06290"
     CDS             2803..3006
                     /locus_tag="PLM83_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOK71049.1"
                     /translation="MWTVVHIAPNRPEAELVKNMLEGEGLLVMLRPIGVPHMGDSANV
                     EVMVPETEAEEACAILDASDRLL"
     gene            3040..4044
                     /gene="pfkA"
                     /locus_tag="PLM83_06295"
     CDS             3040..4044
                     /gene="pfkA"
                     /locus_tag="PLM83_06295"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOK71050.1"
                     /translation="MRRIAVLTSGGDAPGMNPCIRAVVRKSLYHGLEVVGVRRGFHGL
                     VTGDFMEMKSRMVGDILQRGGTILKSARSEEFKTEEGRAKALLQIRTCEIDAVVGIGG
                     DGTFKGLHLLSEMGVPTVGIPATIDNDVGSTEYAIGFDTACNTVLDAINKIRDTATSH
                     ERTYVIEVMGRDSGHIALATGLAGGAESILIPEIEYDIQQVCTRIMDGARQGKSHSIV
                     IVAEGAGGEPPVSTGGAFQEGAAYRIGHQIANITGFETRIIVLGHIQRGGAPSVRDRI
                     LATMFGAKAVDLLLAGEDNAVVGMCANQVRAIPFDEALAMKRPINMDYYELANILSNL
                     "
     gene            4164..4697
                     /locus_tag="PLM83_06300"
     CDS             4164..4697
                     /locus_tag="PLM83_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HOK71051.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDRDGEV
                     ILKKYSPMTDLSFFAQEYADSLYDATGCIAVIADEHEVVAVSGAPKKLFFEKRISEDA
                     ESIMKSGETFVTSTPGEKPVIDGAQFACQVVAPIFAENEPAGVVVLMSKEPREMGTTE
                     TKLVETAAAFLGKQLLH"
BASE COUNT         1202 a         1157 c         1563 g         1171 t
ORIGIN      
        1 gatgcgcgtt caggatttag tcaatcgtca gttagtgcaa ttgggcagga agttgggcgt
       61 gccggttgta gccaccaatg acgtgcacta tttgcggcgt gaggacgcta cagcccatga
      121 tgtgctcctt tgcatacaga ccggctcagt agtggatgac ccgtcgcgca tgaggtttcc
      181 gactgaccaa ttctatctca aagccgcgga ggagatgtac agggagtttg cagagatccc
      241 tgaggcatgc gcgatgacag cgcgcatagc agaacgctgc aatgttgagt tggattttga
      301 tacgttgcac atgcctgagt tcgaggcccc cccggggcag actctcgaca gttacttgag
      361 gagactgtgc gaggagggag cgataaggcg tttcggcgcc atatcccgcg aggttcggga
      421 gaggctcgac tacgaattgg ggataatcga gcgcatgggc tactcgggct atttcttgat
      481 tgtgtgggac ttcatacgct atgccaagag ccaaggcata tatgtaggac cgggcagagg
      541 ttcagctccg ggcagcctgg cagcttactg cctggggatt acagatgtgg atcccttggc
      601 atacaacctc ctttttgaga ggtttctcaa tcctgaacgg gtgacaatgc ccgatattga
      661 cgtagacttt tgctatgaac gtcgcggaga agtcattgat tacgtggcag ggaaatacgg
      721 ttctgacaag gtggcccaga ttataacttt cgggactatg gcggcgcgcg ccgccatcag
      781 ggacgttggt cgggccctca agttcagcta cgcagaggtt gaccgcattg ccaaactcgt
      841 tcctgccgag ctcaacatga cgattgatcg ggcacttgaa gtctcgccgg agcttgctgc
      901 tgcagtggga gaaagcgaac gcaatcgaac gttgatagag attgcccgcg ctgtggaggg
      961 tctgccacgc cacccttcgg ttcatgcagc aggggttgtt atctcaaaag atccgctcac
     1021 agatcatgtt ccgctataca aatccgctga cggcgttctg acgacgcagt atcccatgga
     1081 ggaccttgaa cgccttggca tagtcaagat ggattttctc gggctgcgaa cactcacggt
     1141 gatcgggaat accgtggaaa ttgttaagca cacccgcaat gaggacgtgg acatcgagag
     1201 tattcccctc gatgatccta tggtttatga gatgctgagg aacgcggaat cggaaggcgt
     1261 attccagctg gaaagcagct tgtttcagaa catgcttcga gaggtgaagc ctacgaaatt
     1321 cgaggacctc gtcgccattg ttgccttggg ccgtccgggt ccaatggtga tgacagggga
     1381 tttcgtccgc ggcaagcatt gccatgacac ggtcaagtat ccccacccgg cactggagaa
     1441 aatactcgag cccacatacg gtgtgatgct ctatcaggaa caggttatgc aggcagcgag
     1501 tgagcttgcc gggttcagcc tcggggaagc cgacatgctc aggcgcgcca tgggcaagaa
     1561 gaagccagag gttattgcgg gtttgcggga cagattcatg gaaggggctt taggacgggg
     1621 cgtgtcggaa cgcgcggcac aggaggtttt ctcactgatt gaaaggtttg cgggatacgg
     1681 tttcaataaa tctcatagtg ctgcatatgc cctaatatcc tacagaaccg cctatttgag
     1741 gtgtcactac tttcccgaat tcatggcctc tacactaact agtgtgatgg ggtcgtcgga
     1801 acgcgtggcc atgtatatag acgtgtgcag agccgcaggt gtggacgtgc ttccgcctga
     1861 tgtgaatgag agctttaagg gatttactgt ctcgggcaat acgatacggt tcggccttgg
     1921 tgctatcaag aatgtgggcg agggcgccgc tgaatctatc atatctgcca gaaagagcgg
     1981 cggtccgttt gtgtcatttg tggacttctg caacagggtt gatatgacgg cagtcaacag
     2041 aaaggctctg gagagcctga taagatgtgg cgcattcagc gaattcggaa agaggcgcgc
     2101 ccttcttgcc attatggacc aggtatgtga ccagagtgcg gttcgccagc gccatcaaga
     2161 gtcagggcag gcgtccttct tcgacctgtt tgaagagcct tccggttttg gtactgccga
     2221 cattcccctg ccggatgtac ctgatttcag tgaatcgcag atactggcaa tggagaagga
     2281 attgcttggc ctctatatct caggccatcc gttggcttcg gttgcagagg ccatccgcaa
     2341 agtggccacc atgtcagttc gcgatctgtc aaacgcggag gacggcgcgt atgcgacgct
     2401 tgccggtgtg atcatcggac gaaagcagat aattgcacgg acagggcagc ccatggcttt
     2461 tgtgcagata gaagacttga ctggccaagt ggaggtagtg gtttttccca gaacttatca
     2521 ggaatgcgca gatattcttg cccgcgatgc catcgtcgtg gtaaaaggcc gtgtggacgt
     2581 caaagaggaa ggaatcaaga ttttggccga ttccatcacc gaacttccga aaatactcgc
     2641 caacgcctga aatctttgat accatacttg gatgggagcg gttgcgctgg gcaggactgc
     2701 atgacaacag gctacatgcc ggactgtgag aaggaatcgc tgaagatgga tagaagaacg
     2761 gacatactaa agcggaagat tcagcgaggg ggattaaacg tgatgtggac ggtagtgcat
     2821 atcgcgccta ataggccaga ggcggaactc gtgaagaata tgcttgaagg agaaggcttg
     2881 ttggtgatgt tgaggccgat tggagtcccc catatgggcg attcggcgaa cgtggaagtg
     2941 atggttccag aaactgaagc tgaagaggcc tgtgctattc tggacgcttc cgatagacta
     3001 ctttgatgac agtgggcggg gacgattggg ggaattatca tgagacgcat tgcagtgctg
     3061 accagtggag gcgacgcgcc cggcatgaat ccatgcattc gtgctgttgt gcgaaaatca
     3121 ctctatcacg ggcttgaagt ggtcggtgtc agacgcggct ttcacggact ggtcacaggc
     3181 gacttcatgg aaatgaaatc acggatggta ggggatatac tgcaacgcgg tgggactatt
     3241 ctgaaatcag ctcgttcgga agaattcaag actgaagagg gtcgcgccaa ggcgctcttg
     3301 cagataagga cgtgcgagat cgacgcagtt gttggaatcg gcggtgacgg caccttcaag
     3361 ggtttgcatt tgctctctga aatgggcgtt ccaacagtgg gcattccagc tactatcgac
     3421 aacgatgtcg gatcaactga atacgccatc gggtttgata cggcatgcaa cactgttctg
     3481 gatgcgataa acaagatacg cgacacggcg acttcacatg aacgcacata tgtaatagaa
     3541 gtcatgggaa gggactcagg ccatattgcc ttggcgactg ggcttgccgg gggtgcggag
     3601 agtattctta ttccagagat cgaatacgac atacagcaag tgtgcacaag gataatggac
     3661 ggcgcccgcc aggggaaaag ccacagcata gtgattgtgg cagaaggggc cgggggcgaa
     3721 ccccccgtct caaccggcgg agcttttcaa gagggcgctg catacaggat cggacaccag
     3781 attgccaaca taacggggtt tgagaccaga atcatcgtgc ttggacatat tcagcggggc
     3841 ggtgctccat cggtgcgtga ccgcatactg gccacgatgt ttggcgccaa ggcggtggac
     3901 ttgcttttgg cgggtgagga caatgcagtt gttggcatgt gcgcaaacca ggttcgtgcg
     3961 attccctttg atgaagccct ggcaatgaag cgcccgatca acatggatta ttatgagttg
     4021 gccaatatcc tttcgaattt gtgatgacac gcgggtgcgc aacgtggcat gattggtgct
     4081 gagtataggt tgtagtatac gttatcaaca ataaaggtac aatagagcct gaaatggcgg
     4141 aaatcagttg gggagggcat gaagtgaagg caacaggcat agttaggcgc atcgatgacc
     4201 tcggtcgggt tgtaataccg aaggaaatac gaagaacgct gaggatccgc gagggcgatc
     4261 cacttgagat atttactgat cgtgacgggg aagtcattct gaagaagtac tctcccatga
     4321 ccgacttgag tttctttgca caggagtacg ctgattctct ctatgatgcc acgggctgca
     4381 ttgcggtgat tgcagatgag cacgaggtcg tagcggtgag tggagctccg aagaagctct
     4441 tctttgagaa gaggatttct gaagatgcgg aaagcatcat gaagtccggg gagactttcg
     4501 tgacaagcac gccgggtgag aagcccgtaa ttgatggggc gcaatttgcg tgccaggttg
     4561 tggctccgat atttgccgaa aacgaacccg ccggagtcgt tgttctaatg tcgaaggaac
     4621 ccagagagat ggggacgaca gagacaaaac tagtggaaac tgcagcggcg ttcctgggca
     4681 agcagttgct tcattagtct gtgtgcgcat atagccacct cgccgcggga gatgtcatgt
     4741 cagacatacg ggctctaggc ctggaggctg atacacaccg gatagctggc gattttgcat
     4801 tgcataggta atactgagag tgaagacccc ttcgggatgc tcctgtgggg gttttcttgc
     4861 gccttttatt gcacagagcc accttggtgt tcttgacttg tctggtgttt ctgcacatga
     4921 aggtgtgact ggccccggcc tttgtttggt gacaagtgcg cgaacatgcg gaggttcgat
     4981 gccagtgtca gatgaccgtc tcctcgctat ggttgtgcag gtggtatggc ccacccagcg
     5041 aggaggtggc ctatgcgcag ctcattgagg agattgcact agaggattac atc
//
LOCUS       DAOSAI010000158         5079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10342_length_5079_cov_3.532245, whole genome shotgun sequence.
ACCESSION   DAOSAI010000158 DAOSAI010000000
VERSION     DAOSAI010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10342_length_5079_cov_3.532245"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1501
                     /gene="obgE"
                     /locus_tag="PLM83_06305"
     CDS             203..1501
                     /gene="obgE"
                     /locus_tag="PLM83_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOK71052.1"
                     /translation="MFVDLVEICVRGGDGGHGEVAFRREKYVPRGGPSGGDGGNGGSV
                     IAEADKALTTLTDFRYRKHLRAKNGARGGGSKMTGAAGEDLIIKVPVGTIVRDAETGE
                     MVADLVVHGQRAFLARGGAGGRGNARFATSVRRTPRFAEKGELGEERRLVLELRLLAD
                     VGLIGYPNAGKSTLLSKISSARPKIADYPFTTLSPILGVVDLADKSFVVADIPGLIEG
                     AAEGIGLGHEFLRHIMRTRVLVHVVDVSEESGRDPLEDYRTIRRELELYHPDLPVKPE
                     IVAANKVDMPGASDRARAFSDVLEKEDVQVFPISALTGEGVQSLLYAVADVLAKIPPD
                     DILVEPEEEARVYTQRGSRLRDTVVERDGDVYVLSGEGVERLCQMTDMDNVEAYGRFW
                     AMLAKAGIVDELRKAGAVEGDIVRIGDLEIEYGEAHFWTR"
     gene            1524..2126
                     /gene="nadD"
                     /locus_tag="PLM83_06310"
     CDS             1524..2126
                     /gene="nadD"
                     /locus_tag="PLM83_06310"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOK71053.1"
                     /translation="MKRIGLMGGTFDPVHYGHLVTAEQAYYQFDLDKVVFVPSGQPPH
                     KMGKQITSREHRYMMTVLATTSNPHFEVSSIEVDRSGPSYAIDTVRAFRDRYGYDAGL
                     YFITGADAILEMLEWKDSDDLLDLCEFIAATRPGYALHSDKIEQIFGHRANKIHFLEV
                     PALAISSTDIRERLKDGRPVRYLLPDMVINYIELVGLYRE"
     gene            2204..2461
                     /locus_tag="PLM83_06315"
     CDS             2204..2461
                     /locus_tag="PLM83_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOK71054.1"
                     /translation="MSKTLYVGNLPWGITDHQLQQVFSPYAEVISSRIVTDRDTGRSR
                     GFGFVEVDTDDIEPIVKSLHGTTIDGREITVNEAKAREHRM"
     gene            2561..3193
                     /gene="yqeK"
                     /locus_tag="PLM83_06320"
     CDS             2561..3193
                     /gene="yqeK"
                     /locus_tag="PLM83_06320"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOK71055.1"
                     /translation="MCEDALTSIRDELESTLQPERFAHSLRVEEESRRLAEKYGADVR
                     VCGLAGLLHDCARDLPDDALLTLAHRSGRCDLVESLQNCPMLLHGLAGAVLAREKYGV
                     EDVRVLRAIACHTLGSIEMSAEDKVVCLADYIEPGRKFPGVDRLRALAEHDLDLALLS
                     ALDCTILHLVQTGREIAIETVLARNRLVRAVNTNCEAGDPPAREFGEIAG"
     gene            3183..3560
                     /gene="rsfS"
                     /locus_tag="PLM83_06325"
     CDS             3183..3560
                     /gene="rsfS"
                     /locus_tag="PLM83_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOK71056.1"
                     /translation="MQAELSSTCHAKKAAAAAVEKKAVDVVLIDMTSVSIMADYFLVC
                     TVYTEAHARGVREAIEESMEESKLALRRREGSDASGWVLLDWGDIVVHIFREEERKYY
                     DLEGLWGDAPAVRVGDSSADDAT"
     gene            3921..3996
                     /locus_tag="PLM83_06330"
     tRNA            3921..3996
                     /locus_tag="PLM83_06330"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3954..3956,aa:Ala,seq:ggc)
     gene            4132..>5079
                     /locus_tag="PLM83_06335"
     CDS             4132..>5079
                     /locus_tag="PLM83_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOK71057.1"
                     /translation="MRDRYCFEEIEPKWQAKWEEDQLYKVGEETARPKYYLLEMLPYP
                     SGRIHMGHVRNYSIGDVIARQRSMKGWNVLHPMGWDAFGLPAENAAIEHGADPVTWTR
                     ENIAAMRKQIKRLGFSYDWDREITTCDPSYYKWTQWLFLQLYKNGLAYRKTQKVNWCP
                     SCATVLANEQVVNGACERCDTPVTKRVLEQWFLRITDYAERLLSNLDNMPGWPDKVKV
                     MQRNWIGRSEGVDIDFHVPETGDKLTIFTTRQDTVYGVSYVVVAPEHPLVDKLIEGKP
                     NEAEIRRFVEDTIGQDDIVRTSEETEKVGMFTGAYAVNPA"
BASE COUNT         1195 a         1113 c         1619 g         1152 t
ORIGIN      
        1 agacgtcgtc cgacctcctc aaactcttcg atggtcggtt gatcgtaggc gagcgcgtat
       61 tggagcccgc gaagtaacgt atgggcctgg atcgtgtttg gtcaggcgag gccagcctgg
      121 aggttgattg caggggctgt ggcttttcgg ttcacagttc ctccgaatat gtaggggcgt
      181 gcgggcaagg agagcagttg acatgtttgt tgatttagtc gaaatatgcg ttcgtggcgg
      241 agatggtggc cacggcgagg tcgcattcag gcgggaaaag tatgttccgc ggggcggccc
      301 ttcaggggga gacggtggca atggcggaag cgtcatcgcg gaggcggaca aggctttgac
      361 gacgctgacg gactttcgtt accggaagca tctccgcgca aagaacggcg cccgtggtgg
      421 cggcagcaag atgactggcg cggcaggcga agatctcatc attaaagtcc cagttggaac
      481 tattgtgagg gacgcggaga caggtgagat ggtggccgat cttgttgtcc atgggcagag
      541 ggcattcttg gccagaggcg gggcaggggg gcgtggaaat gctcggttcg cgacatcggt
      601 gcggaggacg ccgcgttttg ctgagaaggg agagcttggc gaggaacgcc gactcgtgct
      661 tgagcttcgg ttgttggcag atgtggggct cattggatac cccaacgcgg ggaaatccac
      721 gttgctgtca aagatttctt ctgctcggcc aaagattgcg gattatccat tcacaaccct
      781 cagcccaatc ctgggcgttg tggatctagc ggacaaaagc tttgttgtgg cggacattcc
      841 tgggcttatc gagggtgcgg cggagggtat aggcttggga cacgagttct tgcgccacat
      901 tatgaggacg cgggttcttg ttcacgtcgt tgatgtatca gaagagtcag ggcgcgatcc
      961 tcttgaggat tatcgaacta tcaggcgcga acttgagctc taccatcctg atttgccggt
     1021 gaaaccagag attgttgcag cgaataaggt ggacatgccg ggggcttctg atcgggctag
     1081 ggctttttcg gatgtgctcg aaaaagaaga cgtccaggtg tttcccatat cggcgttgac
     1141 tggcgaaggc gtccagagcc tgctgtacgc agttgctgat gtattggcca agatcccgcc
     1201 tgatgacatt ctggttgagc ctgaagagga agcaagggtg tacacccaac gtggttctag
     1261 gctgagagat accgtggttg aacgggatgg cgatgtctat gtgctgtcag gcgagggcgt
     1321 agaacgtcta tgccagatga ctgacatgga taacgttgaa gcttacgggc gattctgggc
     1381 tatgttggcc aaggcgggaa tcgtagatga gcttaggaag gccggagctg ttgaaggaga
     1441 tatcgtgcgc ataggagatc tggagataga gtacggcgag gcgcatttct ggacgcgcta
     1501 gcaggtgcgg caggtgtatt acgatgaagc gtataggcct gatgggcggc acttttgatc
     1561 cggtgcatta tgggcacctt gtaacggcgg aacaggcgta ctaccagttt gacctcgaca
     1621 aggttgtgtt tgtgccatca gggcagcctc cccataaaat gggcaaacaa atcacttcgc
     1681 gtgaacacag atacatgatg actgttctgg ccacaacgag caatccccat tttgaggtgt
     1741 cttcaataga agtggacagg tcaggtccat cgtatgccat cgatactgtg agagcttttc
     1801 gagatagata tgggtatgat gcaggacttt atttcatcac aggcgcagac gcaatactgg
     1861 aaatgcttga gtggaaagat tcggacgatc ttcttgacct atgtgagttt attgctgcga
     1921 cgaggccggg gtatgcgctt cacagcgata aaatcgagca aatattcggg cacagagcaa
     1981 acaagattca tttccttgaa gtgccggcct tggcgatatc atccacggat attcgagagc
     2041 ggctcaaaga tggacgaccg gttcgatatc ttctgcctga catggtcatc aactacattg
     2101 aactggtcgg gctatacaga gaatgactgc gcgctgcatc ttgggtgata cataagtgca
     2161 cgggtacggc ggaaaactac gcttaacgga gtgaggtgca ggcatgagta aaactttgta
     2221 tgtgggaaac ctcccttggg ggataacaga tcatcaacta cagcaagtgt tctccccata
     2281 tgcggaggtc atttcaagca ggattgtgac ggacagagat actgggagat cccgcggttt
     2341 tggttttgtg gaggtcgaca ccgatgatat cgagccgatc gtcaaatcac ttcacggaac
     2401 gactatcgat ggaagagaga tcacggtgaa cgaggcgaaa gcccgggaac accgcatgta
     2461 agaaaggatg ttgaagtgca tcgcccccac gtggaacctc tgggggcgat gtgctattat
     2521 gtgatgtagt gaactgtcaa tgctgggggg tagcttcttg atgtgcgaag acgccttgac
     2581 atcgatcagg gatgaactcg aatcgacgct gcagcctgaa cggtttgccc attcgctgcg
     2641 ggtggaggag gaatcgaggc ggcttgccga gaagtacggg gcggatgttc gagtctgtgg
     2701 tttggcaggg ctcctccacg actgcgcacg ggatctacct gacgacgcac tcctaacact
     2761 tgctcatcga agtggccgtt gtgatctggt agagagcctg cagaactgcc cgatgctcct
     2821 tcacggactt gcaggcgcgg ttttggccag agaaaagtat ggagtggaag atgtgcgggt
     2881 gcttcgggca atagcatgcc atacattggg atcaatagag atgagtgctg aagacaaggt
     2941 agtgtgcctt gccgattaca ttgaacctgg gagaaagttc ccgggtgtag ataggctccg
     3001 cgcccttgcc gaacacgatc ttgatttggc tcttctatct gccttggatt gcaccatttt
     3061 gcatctggtg caaactgggc gtgaaatagc catagagact gttttggcgc gaaaccgcct
     3121 tgttcgcgcg gtgaatacta attgtgaggc tggtgatcca cctgctcgtg aatttggaga
     3181 aattgcaggc tgaactctca tccacatgcc acgcaaagaa ggcggctgcg gccgctgtgg
     3241 agaaaaaggc agtagacgtg gtcttgatcg atatgacttc cgtttcaatc atggccgatt
     3301 acttcctggt gtgtacggtg tatactgagg cgcatgcgcg tggcgtgcgc gaagcaatcg
     3361 aggagagcat ggaagagtcg aagttggcgc ttcgccgccg cgaaggctca gatgcatcag
     3421 gctgggtgct cttggattgg ggtgacatag tagtccacat cttccgggaa gaagaaagga
     3481 aatactacga cctagaaggc ctctggggcg atgcgccagc tgtgagagtt ggcgactcaa
     3541 gtgcggacga tgcaacttaa cgtcagattc ctggctgagc gccgtgatgt tggaccgatg
     3601 ggcgtctgtg cggcaatggg ctgcacggct aatcgtcaaa gtggtcgttg tcggaagaat
     3661 gtcagcgggt tatgaggcga attggcttga cttaccgaaa cggggcaggt ataatcatag
     3721 ctgcttgata ttttgaaatg caaacgcaat gaagaggagc aataagtgct gtgccgctca
     3781 aagagagccg ggggtgggtg gaacccggtg tgcgggcagc tctgaactcg ccttggagcg
     3841 gtgcaggtga agtggaaaga gattgtagtc tgcaacgggt ggcagcgtta tatgcccaaa
     3901 agacacgctc aagtttgcgt ggggctgtag ctcagctggg agagcgcttg aatggcattc
     3961 aagaggtcga gggttcgatc cccttcagct ccaccatttt attatccatc gcctcgtcgg
     4021 catgagatgc ttgtgcgtga aacaaacagg gtggtaccgc ggatataatt cgcccctgac
     4081 acttggctgt caggggtttt tttgttccct aaagccgaag gaggaagaag aatgagggat
     4141 aggtattgct tcgaagagat agagcccaaa tggcaggcga aatgggaaga ggaccagctt
     4201 tacaaggtcg gggaggagac tgcccgtcca aaatactacc tattggagat gcttccgtat
     4261 ccctccgggc ggatccatat gggtcatgtc agaaattact ctatcgggga tgtaatagcc
     4321 agacaaaggt cgatgaaggg atggaatgtg ttgcacccca tgggatggga tgcgtttggc
     4381 ctacctgctg agaatgccgc catagagcat ggggctgatc cggtgacctg gacgcgtgag
     4441 aatatcgcgg caatgcgaaa gcagataaag aggctcggat tcagctatga ctgggatcga
     4501 gagatcacta catgcgaccc ctcatactac aagtggaccc agtggctctt cctccagctg
     4561 tacaagaacg ggttggcgta tcgcaagacg cagaaggtca actggtgtcc gtcttgcgcg
     4621 actgtgcttg caaatgagca ggttgttaac ggagcatgtg agcggtgcga tacacctgta
     4681 accaaaaggg tgctggagca gtggttcctg agaatcactg actatgccga gagattgttg
     4741 agcaaccttg acaacatgcc tggctggcct gacaaggtca aggttatgca gcgcaactgg
     4801 ataggccgca gcgaaggtgt ggacatagat tttcacgtgc cggagacagg cgacaaactg
     4861 acgattttca ccacacgcca agacaccgtt tacggagtgt cctacgtagt ggtggcgcct
     4921 gagcaccctc tggttgacaa gctgatcgag gggaagccaa atgaggctga gatacgccga
     4981 tttgtggagg atacgatagg ccaagacgac atagtgcgca cgtcggaaga gacggagaaa
     5041 gttggcatgt ttactggtgc ctatgctgtt aacccggct
//
LOCUS       DAOSAI010000159         5043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10441_length_5043_cov_3.783881, whole genome shotgun sequence.
ACCESSION   DAOSAI010000159 DAOSAI010000000
VERSION     DAOSAI010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10441_length_5043_cov_3.783881"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLM83_06340"
     CDS             <1..374
                     /locus_tag="PLM83_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOK71058.1"
                     /translation="PVARLMADQREIEHIGGTMRLGVYPCVLDPASKLAKAYGENVVE
                     ERHRQRYELNWEYAQVLSEYGMRISGTSPEGDMIEAFELMDHPWFVGTQYHPEFKSRP
                     GKPHPLFREFIVKSMEYSKSR"
     gene            656..1939
                     /gene="rho"
                     /locus_tag="PLM83_06345"
     CDS             656..1939
                     /gene="rho"
                     /locus_tag="PLM83_06345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOK71059.1"
                     /translation="MNIAELEKMTVPALHEIAKQLGIPGHSQLRKQELTFEITKAAIQ
                     KEGALWADGILEVLPEGFGFLRVGKMTPTSDDIYISPSQIRRFGLRKGDMVSGQVRPP
                     KDGEKYFALLRVEAVNGLDPELAARRSHFEDLIPVYPDERLKLEHDPKDVTTRLIDLM
                     APIGKGQRGLIVSAPKAGKTTILKKIANALTYNHPELTLLVLLIDERPEEVTDMERSV
                     DGMVVASTFDQPSSNHVRVADMVLERAKRLTEHGKDVLVLLDSITRLARAYNLEEPPS
                     GRTLSGGVDPAALHHPKRFFGAARNIEEGGSLTIIATALVDTGSRMDEVIFEEFKGTG
                     NMELHLDRKLAEKRIFPAIDINKSGTRKEELLMDAEELETMWLLRKAMATTGSTEMTQ
                     LLIDRIQRTKSNEELINIIRKSQFAENVRNWHNSM"
     gene            2169..3311
                     /locus_tag="PLM83_06350"
     CDS             2169..3311
                     /locus_tag="PLM83_06350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK71060.1"
                     /translation="MPKQGLAAFMCVLVISFCVPTFSCGAMAAGPDEVSFSTDVMRRS
                     GALELVKAIFSRSWTILLAALGAKSDVVADWESGLYPRKPRLDPGANIISTLKTESEL
                     IGPSVAATNALDLDAGKQGSSSSFARAQASIVTHRVEQGESLWTIANSYGVSIETISW
                     ANSLANPNKLKVGQVLTFPSVTGVIHEVSRGDTVWTIAKRYGVSRDKIVEANAIADPD
                     ALKVKQSLVIPGGKPPVTSSRIVVASRGSSDGRISGAASSGGSLSWPVSGRITSRFGP
                     RWGRMHNGVDIAVPTGTSVSAAAAGCVIFAGWRGGYGRLVILDHGNGLQTYYGHNSAI
                     TVSRGEQVAAGERIALSGSSGISTGPHVHFEVRAGGKPQDPMGYLP"
     gene            3341..4600
                     /locus_tag="PLM83_06355"
     CDS             3341..4600
                     /locus_tag="PLM83_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK71061.1"
                     /translation="MLSTAVSDHQGRVFRNDNLHAAARTGNTLVELLESDVIPMPEGG
                     SLMRLPSRRPVGIDATSGQFVSAGLEEAVAAVLPQGYTRTFLPAYIGLEGAPQLPLFG
                     YSAVAFAGDQLMVAAIRTDERDTWDPIHYNLPELAEDIERRLRQFPENRILAQLARCA
                     TEYQCFTAQNVFYGRWEGGIPVSPTCNANCLGCISLQASECCPSPQQRIDFVPTVSEI
                     VEVALPHLEKAVGGIISFGQGCEGEPTRQWPTIVEACRELRQRTDAGTINVNTNAGDV
                     HAIEAICDAGVDSLRVSLASARPDYYGRYHRPRGWCLADVESSIIAARSRSLFVSLNY
                     LVFPGFFDQEPEVDALCDLIERCDIQMLQLRNLNIDPDIYADVMLTENVDSDSFYPAG
                     VDEAIAHIRDCFPSLAIGNFTMPIQRK"
     gene            4775..>5043
                     /locus_tag="PLM83_06360"
     CDS             4775..>5043
                     /locus_tag="PLM83_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOK71062.1"
                     /translation="MGSLSDYIESYLKLLLDTGPHGFVEVQRRELAERFRCAPSQINY
                     VLSTRFTVERGYVVETRRGGGGYIRIFKLDDEPETSGIQMVYESVG"
BASE COUNT         1195 a         1240 c         1452 g         1156 t
ORIGIN      
        1 accctgtggc ccggctaatg gctgaccagc gcgagatcga gcacataggc ggcaccatga
       61 ggctcggggt atacccgtgc gtgttggacc cagcttccaa gcttgcgaag gcttatggtg
      121 agaacgttgt agaggagagg caccgccaaa ggtacgagtt gaattgggag tatgcgcagg
      181 tgttgtcaga atacggaatg aggatctcgg ggacgagccc tgagggcgac atgatcgaag
      241 ctttcgagtt gatggatcat ccgtggtttg tgggtacgca ataccatccc gaattcaagt
      301 cgaggcctgg caagccccac ccgctgtttc gggaatttat tgtcaagtct atggaatact
      361 cgaaatcgag ataagcgcag tttatagtcg tcatggaatg aatgtgctgt ggaaataatg
      421 gttagtgagg ttgcttcgcc ggaggatttt gttgttgctg tggcgaatag cgtctctgtg
      481 acatgttgag gaccttcgcg atcaagctaa tgcttcaagc aagtctcatc actaaaacca
      541 atagtatgcc caatcaaata gcctcaatag aagttccctt agcgcacggc ggtagcctgc
      601 gcgccggatg gctctgcctt tagcgcatat atcctgaaag gaagatgcaa cacagatgaa
      661 tattgctgaa ttggaaaaga tgaccgtacc tgcgcttcat gaaatcgcaa agcagctggg
      721 gattccgggt cactcgcaac tccgaaaaca ggagctcacg ttcgagataa cgaaagctgc
      781 cattcagaag gaaggcgcgc tgtgggcaga tgggatactc gaagtgctcc cagaaggctt
      841 cggctttctg cgtgtgggga aaatgacccc cacctctgac gacatctata tatctccttc
      901 gcagattcgc aggtttggct tgcggaaagg ggacatggtg tcaggccagg tccgtcctcc
      961 gaaggacggc gagaagtatt ttgcgcttct ccgcgttgag gccgtaaacg ggctcgatcc
     1021 cgaattagcg gcgcggagat ctcatttcga ggacctcatc ccggtctatc ctgatgaacg
     1081 actgaagctg gaacatgatc ctaaggatgt gacgacgcgc ctcatagacc tcatggcgcc
     1141 catcggcaag gggcaaagag gccttatcgt gtcggcgcct aaagctggta aaaccacgat
     1201 acttaagaag atagcaaacg ctctgacgta taaccaccct gaattgaccc tcctcgttct
     1261 gctcatcgac gaacgacccg aagaagtcac ggatatggaa cgttcagttg acggcatggt
     1321 tgtggcctca actttcgatc agccctcttc gaaccatgtg cgcgttgccg atatggtgct
     1381 ggagcgcgcc aagcgcctca cggagcacgg gaaagacgtt ttggtgttgc tcgattccat
     1441 cacgcggttg gcacgggcat acaacctcga ggaaccgccg tcaggacgaa cactctcggg
     1501 aggtgttgat ccggctgctc tgcatcaccc caagaggttc ttcggtgcgg cgagaaacat
     1561 cgaagaaggc ggcagcctta caatcatagc cactgcgcta gtggacactg gcagcagaat
     1621 ggacgaggtt atcttcgagg agtttaaggg cactggcaac atggagttgc atctcgaccg
     1681 gaagttggcg gaaaagagga tctttccagc tattgacatt aacaagtcgg gaacgagaaa
     1741 agaggagctt ctgatggacg cggaagagtt ggagaccatg tggttgttga gaaaggccat
     1801 ggccacaact ggttccacgg agatgactca gctgcttatt gacaggattc agcgcacaaa
     1861 gagcaacgaa gagttgatca acataatccg caaatcccag ttcgccgaga atgtgcgaaa
     1921 ctggcacaat tcaatgtgag gcgcatcccg caatcaaagt caaggagtat ctgagagttc
     1981 ctcgtctgga gcagtgagct cctgatgagc ccctcgtttc attttcttgg gcttctcatg
     2041 tgtccggaaa tatcggcgtg gacaggcagt cgacgcttag ggatgacctt cttctcgcat
     2101 gtggttataa tatgtaatat ggccatcaac cacgaggctt tggagtcagg cttattggag
     2161 ggagacggtt gccaaaacaa ggattagctg cgtttatgtg cgtgctcgtt atctcttttt
     2221 gcgtgccaac attttcttgt ggcgctatgg cggcgggtcc tgatgaggtg agcttcagca
     2281 ccgatgtaat gcgcagatcc ggtgctttgg aacttgtaaa agccatattc agccgcagct
     2341 ggacgatact tctggcggca cttggagcca agtccgatgt ggttgctgac tgggaaagcg
     2401 gattgtatcc gaggaagccc cgtctggacc ctggtgccaa tataatatcc accctgaaaa
     2461 cagagagcga gctcataggc ccgagcgttg cagcgacgaa tgccctggac ttggacgctg
     2521 ggaaacaagg tagctcttca tcgtttgctc gggctcaagc aagcattgtt acacaccggg
     2581 tggagcaggg tgaaagctta tggaccatcg caaacagcta tggagtgagc atagagacga
     2641 taagttgggc caattcgttg gccaatccca acaaactcaa ggtcggacag gtccttactt
     2701 tcccttcagt tacaggcgtg atacacgagg ttagccgcgg cgataccgta tggactattg
     2761 ccaagaggta cggcgtcagc cgggataaga ttgtcgaggc taacgccatt gcggaccctg
     2821 atgccctgaa agtgaagcaa tccctcgtta ttcccggggg caaaccccca gtgactagta
     2881 gtcgcatcgt ggtagcctcg agagggtcat ctgatggaag aatctccggt gcggcatcat
     2941 cgggcggaag cctctcctgg ccggtttcgg gaaggattac atcgaggttc ggacctagat
     3001 ggggccgcat gcataatggc gtcgacattg ctgtgccgac cggcacatct gtatcggcgg
     3061 ctgctgcagg ctgtgtcatc tttgcgggct ggagaggcgg ctacggacgg ctcgtcatcc
     3121 tggaccatgg aaatggcttg caaacatact acggacacaa ctcagctatc accgtgtccc
     3181 gcggggaaca ggttgcagcc ggtgaaagga tcgccttgtc cgggagttcg gggatttcca
     3241 ctggtcctca cgttcatttt gaggtgaggg cgggcggcaa gcctcaggat ccaatgggtt
     3301 atctgcccta aaagcgccat gttgaaacag gagatgttct atgttatcta cagcagtcag
     3361 cgatcaccaa ggccgagtat tcagaaatga taatctgcat gcggcggcgc gcacaggcaa
     3421 taccctcgtg gagctgttag agtctgacgt aatcccgatg ccggaaggcg gaagtttgat
     3481 gcgccttcct agtcggaggc ctgtaggcat tgatgcgaca tcaggccaat tcgtcagtgc
     3541 tggacttgaa gaggcggtag cagcagtcct tcctcagggc tacactcgca cgtttcttcc
     3601 cgcatacata ggattggagg gggcgccaca acttccgctc ttcggttatt ctgctgtggc
     3661 ttttgccggt gatcagttga tggtagctgc aatcaggact gatgagcgag acacgtggga
     3721 tccgatccat tacaaccttc cagagctcgc agaggatatt gagaggcgac tgagacaatt
     3781 ccccgaaaac cgcatacttg cgcagctcgc cagatgtgca acagaatacc aatgcttcac
     3841 tgcccagaac gtattctacg ggcgttggga aggcggcatc cctgtctcgc ccacgtgtaa
     3901 tgccaattgc ctagggtgca tatcactcca ggcgtcagag tgctgtccct cccctcagca
     3961 acggatagat ttcgtcccca cagtctctga gattgttgaa gtggcgttgc cccatctgga
     4021 gaaagctgtc ggggggataa tcagctttgg ccagggatgt gagggcgagc ccactcgcca
     4081 gtggccgacg atcgtcgagg catgccggga attgcgtcag cggacggatg cgggtacgat
     4141 caatgtgaat acgaatgccg gtgatgtgca cgccatcgag gcgatatgtg atgcaggggt
     4201 tgactcgctc cgcgtgagcc ttgccagtgc ccgccctgat tattatggca gataccatag
     4261 gcctcgtggt tggtgccttg cagatgttga aagctcgatt attgctgccc gaagccgcag
     4321 tttgttcgta tcgctcaact acctggtatt tcctgggttt tttgaccagg aacccgaggt
     4381 tgacgcgtta tgcgatctca tagaacgttg tgatatccag atgctccagc ttcgaaacct
     4441 caacatcgac ccagacatct acgccgacgt catgctcacg gaaaatgtcg attccgattc
     4501 gttttatcct gcgggagtgg atgaagccat cgcccatatc agagattgct tccccagcct
     4561 agcaattggc aatttcacca tgccaattca aaggaaatag cctgtcagga catcccacat
     4621 catgtgcctg gacgcgtctg accttgtttg accaaattgg tcagttgccg gtttgtatag
     4681 ctacaagagg cctttaggca tgttgctctt ttggacagac aagtgtatca tggtactaaa
     4741 aggtcaacaa tggtcagatc aacgggagga cgcgatgggc agtctatcgg attacattga
     4801 gagttattta aaactgctgc ttgacacggg ccctcatggc tttgtggaag tgcaaagacg
     4861 ggagcttgct gagagattca ggtgcgctcc gtcgcagatc aattacgtgt tgtccacacg
     4921 ctttaccgtt gagcgcggat atgttgtaga aaccagacgc ggcggaggcg gctacatcag
     4981 aatcttcaag cttgacgacg aacctgaaac gagtggcatt cagatggtgt acgagtccgt
     5041 agg
//
LOCUS       DAOSAI010000160         5022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10512_length_5022_cov_3.562915, whole genome shotgun sequence.
ACCESSION   DAOSAI010000160 DAOSAI010000000
VERSION     DAOSAI010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10512_length_5022_cov_3.562915"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLM83_06365"
     CDS             <1..196
                     /locus_tag="PLM83_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71063.1"
                     /translation="RRPGRVGSCRILFYLQEPRRIAGALCFSSVKNIEGGLIMEHCDD
                     LAAVMYLEYVEEAGGPKWFD"
     gene            284..2392
                     /locus_tag="PLM83_06370"
     CDS             284..2392
                     /locus_tag="PLM83_06370"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOK71064.1"
                     /translation="MDLGALAFLVPLSAAIALVVAAGLSASIKRKDEGTEKMRQISEA
                     IREGASAYLRRQYRGVILFFAVMFGVLCMLAAMDFLNWFTPFAFLSGGFFSGLAGYIG
                     MRTATQANARTAHAASKSLNEGLQVAFSSGCVMGLTVVGLSLVHLSLWYYLLTWYYSS
                     AQPIADAAQRLSAITSTLLTSGMGASSMALFARVGGGIFTKAADVGADLVGKVEQGIP
                     EDDPRNPAVIADNVGDNVGDVAGMGADLYESYLGAIIATSALGATVGLGVNWVAFPMV
                     LAAIGVFASIIGTTFVRTGEEVEQSLLLAALRRGVNVSAIIVVVASWFAVRAMLGPDF
                     MGIFWSVIAGLVAGVVIGRVTEYYTSADYKPTTAVAASATTGPATVIISGMASGMAST
                     AIPVLVVAVAVIASYYLAGGSSNEVMGLYGIAISAVGMLSTLGITLATDAYGPVADNA
                     GGIAEMAGMPENVRERTDALDSLGNTTAATGKGFAIGSAALTALALIAAYRDQILLLD
                     PGRELVFSLMTPSVLVGVFIGGMLPFIFSALTMQAVGRAAQGIVMEVRRQFQEMPGIM
                     RGKTKPDYARCVDMCTSSAQKEMIAPSLIAIIVPIVSGILLGPEGVGGVLVGTVVTGF
                     LLAVMMANAGGSWDNAKKYIETGEYGGKGSDAHKASVVGDTVGDPFKDTSGPSLNILI
                     KLVSMVSIVFASLIISLSIM"
     gene            2730..2924
                     /locus_tag="PLM83_06375"
     CDS             2730..2924
                     /locus_tag="PLM83_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71065.1"
                     /translation="MSHGALLGIERATEILRQLADGLTDTVVIKARLGYFDQQLALLS
                     VGKTLEGDVDRRVACHRWTG"
     gene            3030..4574
                     /gene="gpmI"
                     /locus_tag="PLM83_06380"
     CDS             3030..4574
                     /gene="gpmI"
                     /locus_tag="PLM83_06380"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOK71066.1"
                     /translation="MQTYRPVVLAILDGWGYSENESYNAIALARTPNIDKLMQDYPYT
                     TLPAHGRAVGLPEGQMGGSEVGHLCLGSGRIVLQDLSYLDHKIETGEFQRNEVLLGAM
                     KEAARKGKALHLMGLVSPGGVHSHQKHIYTLVEMARDVGVRDVYIHAFLDGRDVPPAS
                     ALDYLDELDANLTRLGLGKVATVSGRYYAMDRDNRWDRTKLAWDAIVHSVGPRARAAQ
                     DAVRASYDSGITDEFVMPTVIVDESGAPVGPVREGDSVIFFNFRGDRARQLTKAFNFE
                     EFSEFDRGERPHVHYVCMMKYLNADIPVAFSPPEPVDGLAETISKEGKAQLHVAETEK
                     FAHVTFFFNGGREEPFPNEDRVLIPSPKVATYDLAPEMSAREIAAEVVSRIEAGKYDF
                     IVVNFANGDMVGHTGKLDATIAAVETVDDCVGQVWRAVKEARGAMIITSDHGNADQML
                     DPDTGQPSTAHSLNKVPFILAGTKATQLRDGGTFADVAPTILELMGIQPPAAMTGRSL
                     IAGYGE"
BASE COUNT         1121 a         1192 c         1507 g         1202 t
ORIGIN      
        1 gagacggcct gggagagtag gtagctgccg gattcttttt tatcttcaag agcctcggcg
       61 tattgccggg gctctttgct tttcttcagt caaaaacata gaagggggcc tgatcatgga
      121 gcattgtgat gaccttgcgg cggtaatgta cttagagtat gtggaagagg ctggcgggcc
      181 caaatggttt gactgacata ttcgggctgt gttatcctag gattgttagt tagctggacg
      241 gagggaggga attacacccg atgttcgtgt cgggtggagg gtaatggact taggagcatt
      301 agcgttcttg gtgcctttga gcgcggcaat cgcattggtt gtagcagctg gcctgtctgc
      361 ctccataaag cggaaggatg agggaaccga aaagatgcgc caaatctctg aagctatcag
      421 ggagggcgca tctgcgtatt tgaggcggca gtatagaggg gtcattctat tttttgcagt
      481 aatgtttggt gtgctttgca tgctggctgc aatggatttt ttgaactggt ttacgccttt
      541 tgcgttcctg agcggcggtt ttttctccgg ccttgccggg tatatcggaa tgcgcactgc
      601 cacacaggct aacgctcgaa ctgcccatgc tgcctcgaag agcctgaatg agggtttaca
      661 agtcgcgttt tcgtcaggct gcgtcatggg tctgactgtt gtggggttgt cccttgttca
      721 cttaagctta tggtattatc tccttacgtg gtattacagc tctgcgcagc ctatcgcaga
      781 tgctgctcag cgcctatcag caatcacctc cacacttctc acgtccggaa tgggagcaag
      841 ctcgatggcg ctctttgcca gggtgggcgg aggcattttc accaaagctg cggatgttgg
      901 cgctgactta gtcggaaaag tggaacaagg catacctgaa gacgatccgc gcaaccccgc
      961 tgtcatagcc gataatgttg gtgataatgt gggcgatgtt gcaggaatgg gcgcagacct
     1021 ttacgagtcg tatctgggtg cgataatcgc cacctcagcc ttgggtgcga ccgttgggtt
     1081 gggagtgaac tgggtcgcat tcccgatggt cctggcggcg ataggggtgt tcgcatcgat
     1141 tattggaacc accttcgttc gcacaggcga ggaggtggag cagtcgctgt tgctagcggc
     1201 actccgccgg ggcgtgaacg taagcgcgat catcgtggtg gtagcgagtt ggtttgcggt
     1261 gagggcgatg ttggggcctg attttatggg catattctgg tcagtcattg caggcctcgt
     1321 tgcaggcgta gtaattggcc gggtcactga atactataca tcggcagatt acaagccaac
     1381 aacggccgtg gctgcttccg ccactaccgg tccggctaca gtcataatat ctggcatggc
     1441 ttctggcatg gcttcgacag ccattcctgt cctggtagtt gctgttgcag tcatcgccag
     1501 ctactatctg gcgggtggat ctagcaacga ggtaatgggc ctttacggaa tcgccatatc
     1561 agctgtggga atgctctcca cgctcggaat cacattagct acggatgctt acggtcccgt
     1621 ggctgacaat gcagggggca ttgcagagat ggcgggcatg ccagaaaatg taagggagag
     1681 gacagatgct ctggactctc tgggcaacac tacggctgcc acgggaaagg ggtttgccat
     1741 cggttcggca gcccttacgg cgctggctct tatcgcggcg tatcgagacc agattcttct
     1801 gctcgaccca ggccgtgagc tagtcttcag tctgatgacc ccgtctgttc tagttggggt
     1861 gttcatcggg ggcatgcttc cgttcatatt ctccgccctc acaatgcagg cggttggaag
     1921 ggcggcgcag ggaattgtca tggaggttcg caggcagttc caggagatgc ctggaataat
     1981 gcgcggaaag acaaagcccg attacgccag gtgtgtggat atgtgcactt catctgcgca
     2041 gaaggaaatg attgcgccat cgctcattgc catcatagtc ccgatcgtca gcggcatctt
     2101 actcgggccc gagggggttg gaggagtcct cgtgggtacc gtggtcacag gattcttgct
     2161 tgcggtgatg atggctaatg cgggagggtc ctgggataat gccaagaaat acatagaaac
     2221 cggcgaatac ggcggaaaag gctctgatgc acataaggca agcgtagtcg gtgacactgt
     2281 tggagacccg tttaaggaca catctggtcc gtcattgaac atacttatca aacttgtctc
     2341 catggtgtcg atagtcttcg cttccttgat aatatctttg tccatcatgt aaagcgcgcg
     2401 cctgttaccc gcgggcggtc ctttcgtgat cggacggtgc cggcctgctc cttgaggagt
     2461 aggccggttc aaactgtaca tgatagattt cagataagca cttgctctgc tgcgagcaaa
     2521 gcaaaacggc catttcaata aagtgaatgc aacttggcag gggtgtatgt cctagtcaaa
     2581 tgactcgcta gcgggccgct aagccgtaaa atagctttca ggcaatgcac acgattggtt
     2641 ggtcctgacg gtttcccaaa ggatgccaca tatccgccca aaactcaaat cccgacacaa
     2701 atggggatgt caaatggaaa ggtccccaaa tgtctcacgg ggctttacta ggcattgaac
     2761 gcgcaacgga aatccttaga cagcttgcag acggtcttac ggatacggta gtgataaaag
     2821 caaggctagg ctacttcgac cagcaactgg cacttctgag tgtaggcaaa actcttgagg
     2881 gagatgttga tcgcagagtt gcttgccata gatggacggg gtagaggcat ggccgtgaag
     2941 gggcgacatc cagttgaacg catattgtgc gtgtgatacg ataatcctgc tgtcagctgg
     3001 gaatctcagc tgcttggggg tgtaaattga tgcaaacata caggccagtc gttcttgcaa
     3061 ttttggatgg ttggggctac tcggagaatg aatcctataa tgccatagcc ttagcccgaa
     3121 ccccgaatat cgacaagcta atgcaggact atccctatac tactcttccc gcccatgggc
     3181 gcgctgtggg acttcctgag ggacagatgg gcggttccga agtcgggcac ctatgcctcg
     3241 gttcaggaag aatagtcctc caggacctca gttacctgga tcacaagata gagacgggcg
     3301 agtttcagcg aaatgaggtc ttgctcgggg cgatgaaaga ggcagctcgc aaaggcaaag
     3361 ctcttcacct tatgggcctt gtgtctccag gcggggtgca ttctcatcaa aagcacatat
     3421 acacgctggt tgagatggcg cgtgacgttg gcgtcaggga tgtatatata catgccttct
     3481 tggacgggag agatgttccg cccgcaagcg ccttggacta cctggatgag ctcgatgcca
     3541 accttacccg gcttgggctt gggaaagtgg caacggtcag cggacggtac tatgccatgg
     3601 atcgggataa ccgttgggac aggactaagc ttgcgtggga cgcaatagtc cattctgtgg
     3661 gcccccgagc tcgcgctgca caggacgctg tcagagcttc atacgattcg ggtataacag
     3721 atgaattcgt gatgcccacg gtgattgtgg atgaatcagg tgctccagtg gggccggtgc
     3781 gcgaaggaga ctcagtaata ttcttcaact tccgcgggga cagagccagg cagctcacga
     3841 aggctttcaa ctttgaggag ttcagcgagt ttgaccgcgg agaaaggccc catgttcatt
     3901 acgtgtgcat gatgaaatac ctaaatgccg atatcccagt ggcattttcg ccgccggagc
     3961 cagtcgatgg gctggcagaa acgatctcga aggagggcaa ggctcagctg catgtggccg
     4021 agacagaaaa gtttgcccac gtcacgttct tttttaacgg aggccgcgag gaaccattcc
     4081 ccaatgagga cagggtgctc atcccgtctc cgaaagtggc aacgtacgac cttgcgcctg
     4141 agatgagcgc cagggagata gctgccgagg tcgtgtcgag aatagaggcg gggaagtacg
     4201 attttatcgt tgtcaatttt gccaacggcg acatggttgg gcacacaggc aagctcgacg
     4261 ctaccatagc agctgttgag actgtggatg attgcgttgg gcaggtatgg agagcagtaa
     4321 aggaagcccg gggcgcaatg atcatcactt ctgaccatgg gaacgccgat cagatgctgg
     4381 accccgacac agggcagcca agcacagcac attccctcaa caaggtcccg tttattttgg
     4441 caggcaccaa agcaacgcaa cttcgcgacg gtggaacctt tgcagatgtg gcccctacga
     4501 ttctagagct tatggggatt cagccgcctg cagctatgac tggaagatcg ctcattgcgg
     4561 gatacggaga ataatgaaac tgaaccgtct tgtagctata cgccaaggct cttgcctgaa
     4621 ggcatgtgcc gatttgggag ccggttgggc ttcgacgctt gttcataacg tcaactgcag
     4681 cagggcagtc cgagtagatt atgagttctc gggcgcggtg tttccgtccg atagtgaggg
     4741 ccatgagtat cgctagaagg gatgcatcga tatagtcgac gggaggagcg tgattttcgc
     4801 tgtgcgaccg gagtggatat gcggcttcgg ataccacttt gccagagctg ttttctgcga
     4861 gcagcacagc tttccatggg cccctatcgt gactgccgct tgagacggct gcaacgtata
     4921 ttcgcaatgg gcgtgccatg ttgttacaac cccctaactc ttgggcaaca aggagaacac
     4981 atgttctacg actagatatt aacatagaac gtatgttcgt gt
//
LOCUS       DAOSAI010000161         5018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10522_length_5018_cov_4.086037, whole genome shotgun sequence.
ACCESSION   DAOSAI010000161 DAOSAI010000000
VERSION     DAOSAI010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10522_length_5018_cov_4.086037"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLM83_06385"
     CDS             complement(<1..653)
                     /locus_tag="PLM83_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK71067.1"
                     /translation="MVVLKATRLSKTYGIETIFKNASFDISLGERVALFGPNGSGKST
                     LLRLITGDEEPDDGQVWIDPKMRVGYVPQKLEFPESATPLFLCQVASLGLPDVGNAEE
                     ALSRFGISKKMRMRRVGTLSAGERTRLGLACAWVIRPDILLLDEPTCHLDIEGMELLT
                     DLLLSHKGAALIVSHDRRFLDDVAMRVLELAPDGIRSFTGNYSAYRMQKEAELDAQM"
     gene            complement(973..2109)
                     /locus_tag="PLM83_06390"
     CDS             complement(973..2109)
                     /locus_tag="PLM83_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOK71068.1"
                     /translation="MNKSQVSITRCKDYEREHVKEALSEALSPLGGMGTFIEPGSRVL
                     IKPNLIAGTPPERGATTHPEVLRAVIEEVRSVGGKPIVGESPGFESFHVAASKSGLLE
                     VLEEMGVDAAPFETSRNVPLSPGHIVTSLPIAEPVVDADAVISLPKLKSHGLVMYTGC
                     VKNMFGVICGSQKGKYHLRFQDRELFSTLLAEIYATAKPTLSILDGIIGMDGNGPRNG
                     DPFDLGIIMAGSDGVAIDAVACSIVGINPVESPPLRIASEKGYGTIDLGNIDIYGPSI
                     EELQVQGFRVPKAARPSMRQRLMSGETGRVLRNWFTTRPSVVRNSCRGCRVCARACPA
                     DAIEIRDGVAHMDYDKCIRCYCCQELCEYGAITLKTPPLARIFR"
     gene            2181..2255
                     /locus_tag="PLM83_06395"
     tRNA            2181..2255
                     /locus_tag="PLM83_06395"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2213..2215,aa:Asn,seq:gtt)
     gene            complement(2571..4190)
                     /gene="groL"
                     /locus_tag="PLM83_06400"
     CDS             complement(2571..4190)
                     /gene="groL"
                     /locus_tag="PLM83_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392083.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOK71069.1"
                     /translation="MAAKQLAYAEEARTSLKNGVDAVASAVKVTLGPKGRNVVLERKY
                     GSPVITKDGVTVAKEIELEDPYENMGAQLVKEVASKTNDVAGDGTTTATVLAQIIVNE
                     GLRNVTAGANPMFLKRGIDKAVELVVDELHKLAIPVDGKEDIAHVASISGNDEAVGEW
                     IAEAMDKVGKDGVITVEESKGIETTVEQVEGMEFDKGYISPYFVTSTESMEAILEDPY
                     ILLYEKKISNVQELLPLLEKIARAGRPLLIVAEDIEGEALATLVVNKLRGILNVVAVK
                     APGFGDRRNAMMEDIAVLTGGEFISEATGAKLENVDLKQLGQAKTVKVNKEKTTIIEG
                     RGEKAKIEARVDQIRREIDDSDSDYDREKLQERLAKLAGGVAIIKVGASTETELKEKK
                     HRIEDALSATRAAVEEGLVAGGGATYINMISKLDQIEAEGDEKTGVNIVKRALEEPLR
                     TIANNAGLEGSVVVERVKSENKPGFGLNALTGEYCDLIKAGIVDPVKVSRSALQNAAS
                     IASMMLTTEAVIAEIPTEEPPMPYPPGGMDY"
     gene            complement(4254..4538)
                     /gene="groES"
                     /locus_tag="PLM83_06405"
     CDS             complement(4254..4538)
                     /gene="groES"
                     /locus_tag="PLM83_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOK71070.1"
                     /translation="MVKPCADRVLVKPHTEEEKTPGGIVLPDTAKERPQWGEVKAVGP
                     GRWDEEGKRRIPLEVNVGDTVIFAKYGGTEIKIDGEEYLILRESDILAVK"
     gene            4742..>5018
                     /locus_tag="PLM83_06410"
     CDS             4742..>5018
                     /locus_tag="PLM83_06410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOK71071.1"
                     /translation="MAELLKLLAISLIPGILWVIYFHRKDIYDKEPARLVAKAFLAGA
                     VMIIPAGLVEAPLRGVLTNPPSLPVLLLASVFGIGLVEEFFKYLAVRR"
BASE COUNT         1047 a         1370 c         1275 g         1326 t
ORIGIN      
        1 ttcatttggg catcaagctc agcttctttt tgcattcggt atgcactgta attgcctgtg
       61 aatgaacgaa ttccatctgg cgctagctcc aggacgcgca ttgctacgtc atctaggaaa
      121 cggcggtcgt gagagactat cagagcagcg cccttgtgcg acagtaagag atcggtcaac
      181 agttccatcc cttcgatatc caggtgacaa gtgggctcat ccagtagcag gatatcggga
      241 cggattaccc aagcgcaagc tagtcccaga cgagtccgtt ctcctgcgct caaggttcct
      301 acgcgccgca tccgcatctt ctttgaaatt ccaaatcgtg acagagcctc ttcggcattt
      361 ccaacatcag gtagccccaa actcgccact tggcatagga agagaggggt tgccgactct
      421 ggaaactcta gcttttgggg aacatatccc acacgcattt ttgggtcgat ccacacctgc
      481 ccgtcatcgg gctcctcatc tcctgttatc aagcgaagca acgtactctt gcctgatccg
      541 tttgggccga ataaagctac acgctcacct aaagagatgt caaacgaggc gttcttgaat
      601 attgtctcta ttccgtaggt ttttgaaagg cgggttgcct ttaaaacaac catagtatcc
      661 actcccaaac ccatgaattc catggcaagt gggactgggc actctcacga gcatagggct
      721 ttgcggaaac aaaatggcta caccgatgct gcaagccttc gggtgtagcc atccaatctg
      781 ctatttttgc gaggttgcca gcattcccac aaatgctcaa acgccgcagg cccccatggc
      841 gctgcgtgca ttgagcgtat tcgcttaagc tagaatgcca gcttactgaa catgtatatg
      901 ttacctctcc gcaaagccta tgttaagaac tgaatacatg ttagcatata ccggatttca
      961 tgtaaactgc ttctatcgaa aaatgcgggc gaggggaggg gtcttgagag taatggctcc
     1021 atactcgcat agctcctgac agcagtaaca ccggatgcac ttgtcgtaat ccatgtgtgc
     1081 tacaccatcg cgtatctcaa tggcgtcagc tgggcatgcc ctggcgcaga ctctgcatcc
     1141 acggcacgaa ttgcgaacta ctgagggccg agtcgtaaac cagttccgta gtacccggcc
     1201 tgtctcgccc gacatcagcc tttgccgcat ggaaggacgg gcggccttgg gaacccgaaa
     1261 cccttgcact tgcagttctt caatggacgg gccgtaaata tcgatgttgc ccagatctat
     1321 ggttccgtag cccttctctg aagcgatgcg aagcggcggc gattcgacgg ggttgatgcc
     1381 gacaattgag cacgcaacag cgtctatggc taccccgtcc gatcctgcca taataatccc
     1441 gagatcgaat gggtctccgt ttcgcgggcc gttcccgtcc atgccgatta taccgtcgag
     1501 tattgaaagc gtcggtttgg ctgtggcgta aatttcggcg agaagtgtcg aaaacagctc
     1561 tcgatcttga aacctcagat gatacttgcc cttctgggat ccgcatatta ctccgaacat
     1621 gttctttacg caccctgtat acatgacgag cccgtgggat ttcagctttg gaagactgat
     1681 tactgcgtcg gcgtcaacta ccggctcggc tatgggcagg gatgttacta tgtggccggg
     1741 gctgagcggc acattccttg acgtttcgaa cggagccgca tctacaccca tctcttcaag
     1801 cacttctagg agtccggact tgctggcggc cacatggaag ctttcgaacc cggggctctc
     1861 gccgactatt ggtttgccgc cgaccgatct cacttcctct attaccgccc tgagaacctc
     1921 tgggtgtgta gtagctccgc gctctggagg cgtcccggca ataaggttgg gcttaatcaa
     1981 aactctgctg ccgggctcga taaacgtgcc catgccgccg agaggcgaga gcgcttcact
     2041 gagggcttct tttacgtgct ctcgctcata gtccttgcat cttgtgatgg aaacttgaga
     2101 cttgttcaat gcagtacctc cgtaactagt tttgcctctt gactacactt cgttgggctg
     2161 ctattataaa ttcataacgt tcctcgatag ctcagcggta gagcaaccgg ctgttaaccg
     2221 gtgggtcgca ggttcgaatc ctgctcgggg agccattttt ttgtacattt ccgtccgtag
     2281 tttctccttc cacagaggcc cacctgttac gcttcaaccc cagcaaggtt ggggccgcaa
     2341 gagatgaaca tggcgaagtc cagcggctca attcagcgaa atgaatggcg ccaaaggggc
     2401 catgctctat gtaatcggca atatacttgc agatgggcag aaatctgggt tccatgccta
     2461 tggaatgctt atatgatacc acaggaagaa aagggcgtga atgaaaagag gacgcgggct
     2521 atcccggtat tgcagggaat gcccgcgctt gttttagtat tggcgaacgt ctagtagtcc
     2581 attcctcccg gaggatacgg catcggcggc tcttccgtgg gaatctcggc gatgacagcc
     2641 tcggtggtga gcatcatcga tgcaatggag gctgcgttct gcagggctga cctgctgacc
     2701 ttcacggggt cgactattcc tgccttgatt agatcgcagt attccccagt cagagcgttc
     2761 aagccgaacc cgggcttgtt ctctgatttc actctctcga ctacaacaga gccctcaagg
     2821 ccggcattgt tggcgatggt gcgcaacggt tcctcgagag cgcgcttgac gatattgact
     2881 ccggtcttct catcgccttc tgcttctatc tgatcgagtt tggaaatcat gttgatgtag
     2941 gtggcgccgc cgcctgcaac tagtccttcc tcaacggccg cccttgttgc cgaaagagca
     3001 tcttctattc tatgcttctt ctccttaagc tcggtctctg ttgaggcgcc aactttaata
     3061 atagccacgc cgccagcgag ctttgcgagg cgctcctgga gcttctcacg gtcgtagtcg
     3121 gaatcggaat cgtcgatttc gcggcgaatc tggtcgacac gcgcctcgat cttggccttc
     3181 tctccacggc cctcgatgat ggtcgtcttt tccttgttga ccttgaccgt cttggcctgg
     3241 ccgagctgct tcaggtcgac gttttcaagc tttgcacccg ttgcctctga gatgaattct
     3301 ccgcctgtaa gcacagctat gtcttccatc attgcgttgc gcctgtcacc aaaccctggc
     3361 gccttaacag cgacaacgtt taggattccg cggagcttgt tgacgacgag agttgcaagg
     3421 gcttcgcctt ctatatcttc ggcaacgatc aaaagcggtc tgccagcacg ggcgatcttc
     3481 tccagcaatg ggaggagctc ttgaacatta ctgatcttct tctcgtaaag cagaatatat
     3541 ggatcttcga gaatggcctc catcgactcc gtgctagtga cgaagtaggg ggatatgtat
     3601 cccttatcga attccatgcc ttcgacctgt tcgacagtgg tctcgatgcc cttggactcc
     3661 tcgacggtga ttactccgtc cttgccgact ttgtccatgg cttcagcgat ccattcgccg
     3721 acggcctcgt cattcccgga aatcgaggct acgtgggcaa tgtcttcctt gccgtccacg
     3781 gggatggcga gtttatgtag ctcgtctacg acgagttcta cggccttgtc tataccgcgc
     3841 ttcaggaaca tcgggttcgc gccagcagtt acgttgcgca gcccctcgtt cacaattatt
     3901 tgggcaagga cagtggcggt tgtggtgccg tcgcctgcga cgtcgttcgt tttcgatgca
     3961 acttccttga cgagctgggc tcccatgttt tcatacggat cctctagctc gatttctttg
     4021 gccaccgtca cgccgtcctt agtgatgact ggtgatccat acttccgctc aagcacgacg
     4081 ttgcgtcctt taggacccaa tgtgaccttg actgcagatg caactgcgtc gacaccgttt
     4141 ttcaggctag tacgcgcttc ttctgcatat gctaactgct tagcagccat tttcgtttgc
     4201 ctccttcata cgcatgaatg atttgcatgt tggtcttcgt ggggcagtag gttctacttg
     4261 accgcgagaa tatcggactc gcggagaatc aagtattctt cgccgtcaat cttgatttca
     4321 gtgccgccgt atttggcgaa tatgaccgtg tccccaacgt ttacctccag tgggatgcgg
     4381 cgcttgcctt cttcatccca tctgccgggc ccaacagcct tcacttcgcc ccactgaggg
     4441 cgctctttag cggtatccgg aagaacaatt ccgcccggag tcttctcttc ttccgtatga
     4501 ggctttacaa gcactctgtc agcacaaggt tttaccatcg acgtttacct ccttcacggc
     4561 gttgctctta gcactcaacg gatgtgagtg ctaacaaaga tatgatatac tcctcgtttt
     4621 ggtgttgtca aggcttatga tagaattatt tccgggatag gagtctatat acagtcgacg
     4681 actgccgttt gtccggaaat actggtactt aaccgaaccg atgaaggatg ggatttggac
     4741 catggctgaa cttctaaagc ttcttgcgat atcgctgatt cccggtatcc tatgggttat
     4801 ctacttccat cggaaagaca tatacgacaa agagccagca cgtttggtcg caaaggcatt
     4861 tcttgcaggg gcggtaatga taatcccggc aggtcttgtg gaagcgcctc tacgaggcgt
     4921 gcttaccaat cctccgagtt tgcctgttct tttgctcgcg tctgttttcg gaataggctt
     4981 ggtcgaggag ttcttcaagt atttggccgt tcggagaa
//
LOCUS       DAOSAI010000162         4999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10576_length_4999_cov_3.079490, whole genome shotgun sequence.
ACCESSION   DAOSAI010000162 DAOSAI010000000
VERSION     DAOSAI010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10576_length_4999_cov_3.079490"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..556
                     /locus_tag="PLM83_06415"
     CDS             47..556
                     /locus_tag="PLM83_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71072.1"
                     /translation="MIDCRYLDALRKIYVHLDGSPVVWAVTGSLGMALQGMSMEVHDI
                     DIQTDRAGAYEIEMRLSSLVSKKVAFSEAANIRSHFGELCVNGIRVEIMGDIQKRLED
                     GSWEEPVDPARYRLFVQQDDMHIPVMSLEYECQAYAKLGRTKKADMIRDWLRIHKLDG
                     NTNEDHDRN"
     gene            complement(755..907)
                     /locus_tag="PLM83_06420"
     CDS             complement(755..907)
                     /locus_tag="PLM83_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71073.1"
                     /translation="MKAVFPKTDYYISDYLFEELSDMKKLPVMLQSIMDADDWRSSQR
                     RACGTT"
     gene            complement(881..1625)
                     /locus_tag="PLM83_06425"
                     /pseudo
     CDS             complement(881..1625)
                     /locus_tag="PLM83_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823548.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(2009..2878)
                     /locus_tag="PLM83_06430"
     CDS             complement(2009..2878)
                     /locus_tag="PLM83_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK71074.1"
                     /translation="MKNAKTFRVILSYAVLCLVAVVMAGPFVWMVFSSVKPNEDLFAH
                     PPVWFPQRVTGEHFRAAFAHTNLPRYFLNSLIVAGVATVTNVLFGSLAGFAFARLEFP
                     GRDVIFFAILGTMMVPIHVTVVPLFILAKRFPLAGGNNILGVGGTGLIDTYGGLLFPY
                     LITAFGVFMTRQFFASLPRELDDAARIDGCSDFGIFAKICLPLSRPVIATLSIFTFTN
                     VWDDFLWPLVIINRDEMRTVQLGLQVFQSQFSVDWGPLMAATLMVTVPVLIVFILGQD
                     YFVEGIATTGMKG"
     gene            complement(2882..3763)
                     /locus_tag="PLM83_06435"
     CDS             complement(2882..3763)
                     /locus_tag="PLM83_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK71075.1"
                     /translation="MSRHKRGNLNGYLFIAPVFIYITITAFIPMIMSLYLSFTDYNML
                     QPPKWCGLYNYKDLLGKDGKMWVAMRNTLIYAIEVLPLNIFCSLGLAILVNKNLPGMT
                     FFRTTYYLPVVTSMVAASMIWMWIYDTDMGLLNNILSLFGVEPIEWLTSPDMALHSLA
                     IMRVWKGIGWNMVIYLAGLQGIPRHLYEAAMIDGASRWQQFANVTWPMLRPVTYYVLT
                     MGLISTFQTFTEIYSMTRGGPLDSTTTIGYQIYKMAFDYMEMGKASALAFVLFVIIFT
                     LSIINVKFFSSRVDSEV"
     gene            complement(3886..>4999)
                     /locus_tag="PLM83_06440"
     CDS             complement(3886..>4999)
                     /locus_tag="PLM83_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK71076.1"
                     /translation="PLFRRAYPNIDLVVTFVPWGDLSTKLGTAFAAKLPPDVFMHGQA
                     ATAGFAANQSIEPLDKYIAKMPDAKDFGPTIDSGAYMGKRYMLPMNGAGRLLIYRADF
                     FREAGLDPNRPPTTWEELRAAAIAQTIRQGNRIVREGLDIPTQGIDAQQIYSNFLWQN
                     GGDFISQDGKKAIFNSKEGVEALQFIVDLIYKDKCTNTQEERPLGNVQPIASGKVAMQ
                     FAVPNDIKLIEVYSPDVYTQIRVAPPLMNKEQVALYSYSGLFMAKDSKNKDAAWEVMQ
                     FMTSPAVLGKICESLGMLPPRQSLKSAPFIAKDYRIQAYVEGMAYGRGNPNVPAWVKV
                     RDILATYIEKAAFGVMSPQDALDKAAAECNAALAGK"
BASE COUNT         1298 a         1311 c         1149 g         1241 t
ORIGIN      
        1 catcagaagg cgcaattctc caatgcgcaa ggggggggaa agcgggatga ttgactgcag
       61 atatttggac gctctgagaa agatatatgt ccatttggac ggttctcccg tggtctgggc
      121 tgtcactggc agcctgggca tggcgctaca ggggatgtcc atggaagtac acgatatcga
      181 tatccaaaca gaccgcgccg gcgcttacga aatagagatg cgcctatctt ccttggtctc
      241 gaagaaggtg gctttctcag aagcggcaaa catccgctca cactttgggg agctgtgcgt
      301 caacggaatc cgggttgaga taatgggaga catccagaaa cgattggagg atggaagctg
      361 ggaagagcct gtcgatccgg cacgataccg tctgtttgtc caacaagatg acatgcacat
      421 tcccgtcatg tctctcgagt atgagtgcca ggcatatgcc aaactgggcc gcaccaagaa
      481 ggcagacatg atccgtgact ggctacgaat ccacaagctt gatggtaaca ccaatgaaga
      541 tcatgaccgc aactaggaca tgtgccactg tttgatcttg gatctcgcct tgacaaacag
      601 tttagcaatt gccgcaaaag caggaaccgt ggcccatact ctgctatggt tttcaagact
      661 gttctggagg agaatagaat ccatatcaag ggtaccccaa gccatcccga gagccggaga
      721 cccgcccatt gatgttgcat cgcctgtttg tcccctaagt agtcccacaa gcccttcttt
      781 gacttgaccg ccaatcgtcc gcatccataa ttgactgcag catcaccggg agtttcttca
      841 tgtcgctcag ttcttcaaaa aggtaatcag atatgtagta gtctgtcttg gggaacaccg
      901 ctttcactgc ctcagagaag ccactcaagc catctttgca tattatcagg atgtcctcaa
      961 cgcctctgga tttgagatga tcacatactc ccagccaaaa gcttgcactt tcctcatcgc
     1021 caatccagat ccctaagaca tctttgtacc ccgagacatt tattcctaac gctgtatatg
     1081 cagccttatt gacgattcta ctgtcctagc ggacattgaa gtaaatggcg tccagaaaaa
     1141 cgatagggta gattcgatcc aatgctcgcg actgccactt tttttatcat tggcacgatt
     1201 ctgtccgtga tgttgctaac aagctcggac gagatattaa tcccatacag ctcccttatc
     1261 tgatcaccta tgtcccttgt agacatggct ttggcatata tagaaatgat ctggttctca
     1321 agcctgctta cgttcctctg attcttcttc actaccttcg gttcgaactc gccgtttcta
     1381 tccctcggaa tagctatttc catctcgccc tgtctggtct tcacgttttt cctgctgtat
     1441 tcgttgcggc tattcccaga gttgtcacca acattgtcgt gcttcacgta accaaggtgc
     1501 tcattcatct cggcttcaag tatctcctgg agagtatccc caaagaggct cttaaggaca
     1561 tcttcaatat cctcaacggt gtggcaattt ctcgctaact caggggcatc tatcaatggc
     1621 cacaaaaact ccccgtggac agggaaacag cgctacaagc aagagccctg tggcggcggc
     1681 cgaatgtgca gacgttcatc ttctgaaaag cgcagctcaa cgtcgaacgg attgctccag
     1741 acatccgtat ccgacgatca gagttcaagg gaaactctgt cacgactttc tccctgaaag
     1801 gctgcgttga aggcggccag aacagcaccg tcgcgagaat acccgaactc cgcagtaact
     1861 ggaaatcccc atatgcaacg cgggatggca gtgcctcttt agtcgctaag gtttcaagtt
     1921 ttgcggacca ttccttgact ctagcggagc ttgtaggccg atggtatatc ggagatacga
     1981 gtgaaccgca gtctcccgac atcaacgttt atcccttcat ccctgtagtg gcaatgcctt
     2041 cgacaaaata gtcctgaccg agtatgaaca ctatcaagac ggggactgta accatgaggg
     2101 tggcagccat tagagggccc caatctaccg aaaattgaga ctgaaatacc tgcaatccaa
     2161 gctgcacggt acgcatctcg tctctattta tgataaccag gggccagaga aagtcatccc
     2221 aaacgttggt aaacgtgaat atggacagtg tagcaatgac tggccttgac aagggaagac
     2281 agatctttgc gaatatgcca aaatcagagc agccgtctat tctggccgca tcgtccagtt
     2341 ccctaggcaa tgatgcgaag aattgccttg tcatgaatac gccgaatgcg gtaatgagat
     2401 acggaaagag caggccgcca tatgtgtcaa tcaggccggt ccccccaact cccagtatgt
     2461 tgttgccacc agccagagga aaccgttttg caagtatgaa caaaggcact acagtgacgt
     2521 gaataggaac catcatcgtt cccaaaatcg caaagaagat cacgtccctc cccggaaatt
     2581 ccagcctggc aaaagcaaaa cctgctaatg acccgaatag cacgttcgtg acagtggcaa
     2641 ctccagcgac gataaggctg tttagaaaat acctcggcaa attggtgtgc gcaaaagccg
     2701 ccctgaaatg ctcgcccgtc acacgctgtg gaaaccagac aggagggtgg gcaaatagat
     2761 cctcgttggg tttcactgag gaaaacacca tccacacaaa aggtcctgcc atgaccacag
     2821 caaccaagca aagaactgcg taactaagga tgaccctgaa tgtcttggcg tttttcatga
     2881 cttagacctc gctatctact cttgaactga agaacttaac attgataatc gatagggtga
     2941 agataatcac gaacagcacg aaagccagag ctgacgcctt acccatctcc atataatcaa
     3001 atgccatttt gtatatctga tatcctattg tggtcgtact atcgagcggc cccccccgag
     3061 tcattgagta tatttctgta aatgtctgga atgtagagat aagacccatg gtaagcacgt
     3121 agtaagttac cggcctaagc ataggccatg taacattggc aaactgctgc caacgactag
     3181 cgccgtcgat catggctgcc tcatagagat gtctaggtat cccttgtaag cccgcgaggt
     3241 aaataaccat gttccaccct attcccttcc acacacgcat tattgcaagg gaatgtagcg
     3301 ccatatcggg tgaggtaagc cattcgatag gttccactcc aaacaggctc aatatgttgt
     3361 taagcagccc catgtcagta tcatatatcc acatccatat catgctcgca gcaaccatgg
     3421 atgtaaccac aggcagatag tatgtcgtcc tgaaaaatgt catgccaggc aggttcttgt
     3481 tgaccagaat cgccaagccc aacgaacaga agatattgag aggcaacact tcaatggcat
     3541 atatgagtgt attgcgcatt gccacccaca tcttgccatc cttgcccagc aagtccttgt
     3601 agttgtatag tccacaccac ttgggcggct gaagcatgtt gtagtcagta aagcttaaat
     3661 aaagagacat tatcatggga ataaaagcgg taatggttat gtagataaat accggagcaa
     3721 taaaaagata accgttaagg tttccacgct tgtgcctgct catgggcgat catctcctct
     3781 ctggctcaac tcgcatcact ctcactgggg agcaaggcat catcgcctac ctttgctccc
     3841 cagtgaaaac tgatatacat gaaagttctg ccgccagtta attacctact taccagctaa
     3901 tgctgcattg cattcggccg ctgctttgtc tagagcatct tgaggactca tcaccccaaa
     3961 agcagccttc tcaatatatg tagccaatat atctctaacc ttgacccatg caggcacgtt
     4021 ggggttgccc cgaccataag ccatgccttc gacgtaagcc tggattcggt agtctttggc
     4081 tatgaacggc gccgacttta gtgattgacg aggcggcaac atacctaagg attcacatat
     4141 cttgcctagt actgctgggc tcgtcatgaa ttgcatgacc tcccaagccg catctttgtt
     4201 cttggagtcc ttcgccatga acagtccgct atacgaatag agagctactt gttccttatt
     4261 catcagtggt ggagccaccc tgatctgcgt gtagacatcg ggagaatata cctctatcag
     4321 cttgatatca ttgggcactg caaactgcat tgcaaccttt ccgctagcga ttggctgcac
     4381 gttacccagc ggtcgctcct cctgagtatt ggtacactta tccttgtata tgagatccac
     4441 tatgaactgc agagcctcca cgccctcttt gctgttgaat atggccttct tgccatcctg
     4501 gctaatgaag tctccaccgt tctgccacag gaaattggag tagatctgct gcgcgtcaat
     4561 tccctgagta ggaatatcta ggccttcgcg aacaatccta tttccttgtc ttattgtctg
     4621 tgctatggcc gccgcacgaa gctcttccca cgttgtcggc ggtctgttgg ggtctagccc
     4681 cgcctcccgg aaaaagtcag cacgatagat cagcaaacgt cctgcgccat tcataggcag
     4741 catgtaacgt tttcccatat aggcccctga atcgatcgtg ggcccaaagt ccttagcatc
     4801 tggcatcttc gctatgtact tgtcaagagg ttctatgctt tgatttgccg cgaatccagc
     4861 tgttgcagcc tgcccatgca tgaatacgtc cggaggcagc ttggcagcga atgccgttcc
     4921 gagtttggtt gaaaggtcac cccagggaac aaatgtaacg acgagatcga tgttaggata
     4981 tgctctcctg aacaatggc
//
LOCUS       DAOSAI010000163         4998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10581_length_4998_cov_3.516083, whole genome shotgun sequence.
ACCESSION   DAOSAI010000163 DAOSAI010000000
VERSION     DAOSAI010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10581_length_4998_cov_3.516083"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLM83_06445"
     CDS             <1..266
                     /locus_tag="PLM83_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOK71077.1"
                     /translation="RAGEYLGVGIANLINVFNPELIILGGQIRPSDTEFIEAARSSAM
                     SRVLIEVGSAVTITASKFGRDAGLVGASALLWKEILRDLAESE"
     gene            328..1251
                     /locus_tag="PLM83_06450"
     CDS             328..1251
                     /locus_tag="PLM83_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOK71078.1"
                     /translation="MADSGRMAVGVDIGGTHLRVGAVRGYEVLAYEKERAWSSDMGPD
                     EVMDIVAEHVRTVVDVAGLDMNEVEGVGVGIPGRVDHESGEVIQAVNASWARETVNVK
                     RMLELRLGLPVMVDHDVAVAAAGEYIYGGHGKSDYFLYVTLGTGIASCYIANGKLLRG
                     AHNSASEMGHTCIDPDGPRCVCGMAGCLERLAAGPAIARAAGMRSEEVVEAARNGEPR
                     AVDVMDRVVNYLALGFVNVINVLDPDVVVIGGGLSQVEDVIVDPLRMRIRKIYRSPFA
                     EELEIKRATFGDDAGIIGAAAQFGQEYADLS"
     gene            1371..2597
                     /locus_tag="PLM83_06455"
     CDS             1371..2597
                     /locus_tag="PLM83_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOK71079.1"
                     /translation="MTCELDRLIGLDGAEKERLGIIFTPSEIRSQASLWRESGETVFN
                     KQTELSGLFVPARPGRPGLHDPFDPCHRRTLILSGAGSSGHVGRCLAPLFRRRLNVHA
                     SVFDTTDIVIDPEGHLIKDEPCLVVLFARSGDSPESLATYEIARSYCKDVRFLAVTCN
                     PEGGLARLAAERPSEVSLILLDPSCNDKSLAMTSSFTNMVVAGQALSFLDAPAEYRKQ
                     AELMHTAAEDLIQEDSVFRELAETPFERAVFLGDGALYGAAAESALKVQELTDGTVMT
                     MSQTFLGVRHGPKAVVNNKTLVVFYVSTDSYKLEYEADLIEQFCRENVAMKTMAVAPN
                     LPKRISDLVNCSVSYGQKAQGEIDDDLRSPIDVIAGQLLGVYKALDLGFKPDAPSARG
                     VISRVVKGVTIYPFWG"
     gene            2777..4075
                     /locus_tag="PLM83_06460"
     CDS             2777..4075
                     /locus_tag="PLM83_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK71080.1"
                     /translation="MRSRLFAVISAVVLLLATCMGVNAAKQQLEYYSMWNPGEDQQQV
                     LAKVIADFEKANPGVSVKVTWAGRQVVAKARSRVIMGTPPDLIDQSFCELWGALLADK
                     EVAVPVNDLLDSRGPEGQKKFRDIFYAPTFDLYQVSGKSYFVPYEFITSGFFYDKTTF
                     KKYGLQEPKTWDDLMDVAGKLKSKGIAPFAQDGNISFYNAYWFYWLTNRILGPGSFYA
                     AAADRTGKTWDHPGYLKAAEMIYDISKARNGYFQKGYDGSNYPAAQMGWARRESAMIV
                     CGTWIPNETAEGRAADFDTGYFGFPEVKGGKGTLQNFEAYMIGFAIPKGAKNEELAKE
                     FIKFTLKKEYQEMIADKAQNIAARKDVKLPAVLADVEPYLRNATEFHMEYDGVQAKLP
                     EWFTSVFYGLDDKLLFGTITPNEFIRQIKQQTINYWQKKK"
     gene            4293..>4998
                     /locus_tag="PLM83_06465"
     CDS             4293..>4998
                     /locus_tag="PLM83_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK71081.1"
                     /translation="MKRNDKTFVIACLVPALVVYVVFFVFPALAALYVSLTDWSGFTP
                     NLEFIGLRNFRELFADEHFWKVVIRNTLGIIFIGGAIIFALAFLFSAILSSGIKSKRL
                     LRSIVFFPNIINPIGLAVMWSFIYEHDSGLLNNFLRAIGLGGLARTWAAPDRLFWSLL
                     VALVWIYVGFFVVILMAGFDRIPNDVIESAKIEGANDVRIFFSIKVPMIWDVLTMAIV
                     LWGIMALKEFAFLYSWG"
BASE COUNT         1173 a         1099 c         1514 g         1212 t
ORIGIN      
        1 atcgcgctgg cgagtatttg ggtgttggga ttgctaacct gataaacgtc ttcaaccctg
       61 aactcataat cttgggaggg caaattcgtc cctcggacac cgagttcata gaagcggcaa
      121 ggagctccgc gatgagtcgg gtcttgattg aggtggggag cgcggtgact attacagctt
      181 ccaaattcgg acgtgacgcg ggcctagttg gagcaagcgc cctgctgtgg aaggagatat
      241 taagggacct tgccgagtca gagtagttct gggacatgtt ctgaattggt cccactggtt
      301 acagctcagg tgtgggagtg atttgagttg gcggatagcg gacggatggc ggtgggggta
      361 gacattgggg gcactcatct ccgcgtggga gcggtgcgtg ggtacgaggt gttggcatac
      421 gagaaagagc gtgcatggag cagcgatatg ggtcccgatg aggtcatgga catcgttgcc
      481 gagcatgtga gaaccgtggt cgacgtcgca gggctcgata tgaatgaggt ggagggtgtt
      541 ggcgtaggga ttcccggtcg tgtggaccat gagtccggcg aggttattca ggcagtaaat
      601 gcaagctggg ctagggaaac agtcaacgta aagagaatgt tggagcttcg cctgggtctg
      661 ccggtgatgg tggatcatga tgttgcggtg gccgctgcgg gagagtacat atacggaggt
      721 catggcaaga gcgattactt tctttatgtc acccttggaa ccggcatagc ttcctgctat
      781 atagccaacg gcaagcttct gcgcggcgcg cacaacagcg cctctgagat ggggcataca
      841 tgtatagatc ctgatggccc tagatgcgtc tgcggcatgg cgggatgcct cgaacgtctt
      901 gctgccggtc ctgccatagc gcgagcggcg gggatgcggt ctgaggaggt tgttgaggcc
      961 gcccggaatg gagagccccg ggcagtggat gtaatggaca gggtcgtgaa ctatcttgct
     1021 ttgggtttcg tgaatgtcat aaatgtgctg gatccggatg tagtggtgat cgggggaggg
     1081 ctttctcagg tggaagatgt gatagtcgat cctttgagaa tgcgcatcag aaagatctac
     1141 aggtcaccat ttgcggaaga attggagatc aagagggcaa cgtttggcga cgacgcaggc
     1201 attattggag ctgccgctca gtttggacag gaatacgcag accttagcta gggtcttcat
     1261 gtgcttccaa gcttcggatc gcagcgtgtg gagcggtttg ctgagcgcgc ccaccctgta
     1321 cggcgtagcc gaactgacgg ctagatcagt ttgggggaga ggatgcatcc atgacgtgcg
     1381 aacttgacag attgatcggt ctcgatggcg ccgaaaagga gcgcttgggc atcatattta
     1441 ctccttctga gataaggagc caagcgagtt tgtggcgaga gagcggggaa acggtcttca
     1501 acaagcagac tgagcttagt ggcctgtttg tgccggctcg gcctgggagg ccggggctac
     1561 atgatccgtt cgatccatgc catcggcgga ctttgattct ctcaggcgct gggagttcgg
     1621 ggcatgtggg gaggtgcctt gcgccgcttt tcaggagaag gttgaacgtg cacgcaagcg
     1681 tgttcgacac caccgatatt gtgattgatc ctgaagggca tctcatcaaa gacgagcctt
     1741 gtcttgtcgt gctctttgca aggtcaggag atagtcctga aagcttggcg acgtatgaga
     1801 ttgcccgatc ctattgcaag gacgtccgat ttcttgccgt gacgtgcaac cccgaaggag
     1861 gcctggcgcg gcttgctgcg gaacgcccgt cggaagtttc cctcattctg ctcgacccta
     1921 gttgcaacga taagagcctt gcaatgacgt cgtcctttac caacatggtt gtagcaggtc
     1981 aggcactgtc tttcctggat gcgcctgctg aatatcgaaa gcaggcagag ctgatgcata
     2041 ctgcggccga ggatctgatt caagaagata gtgtatttcg tgaactcgcg gagacaccgt
     2101 ttgaacgtgc cgtgttccta ggagacgggg cattatatgg agcagcggcg gaaagcgctc
     2161 tcaaagtgca agagcttaca gatgggactg tcatgacaat gagtcagaca ttcctcggtg
     2221 tcaggcatgg acctaaggct gtagtcaaca acaagactct ggttgtcttc tatgtttcga
     2281 ctgattccta caagcttgaa tacgaagccg acctcataga gcaattctgt cgagagaatg
     2341 tggccatgaa aacgatggcc gttgctccta accttccaaa gcgcatatcc gatttggtga
     2401 attgttcggt aagttacggt caaaaagcgc aaggggagat cgatgatgat cttcggtcgc
     2461 cgatcgatgt gatcgcgggt cagctattgg gagtatacaa agctttggac ctaggattca
     2521 agcccgatgc tccatcggcg cgcggggtta tcagccgtgt agtgaaaggg gtcaccattt
     2581 acccattttg ggggtaggtg ctacatgttc ggcagtgagg cgcgtgagag tcgtcgattc
     2641 gaactacagg caaataagaa atacgggggg tggcgctctt actcgaattc gcacctgaag
     2701 ttaacaattt catgtcccta agggaagcga gaacgccgtc ataggagcga cttagcaagc
     2761 gagaggagtg acatcaatgc ggtcacggct atttgcagtg atatccgctg tagtcctact
     2821 gctggctact tgcatgggag tcaacgcggc gaaacagcaa ctcgagtatt actccatgtg
     2881 gaatcctggc gaggaccaac agcaggtctt ggcaaaggtg atagccgact ttgagaaggc
     2941 aaatccaggt gtgagcgtaa aggtgacatg ggcaggcaga caggtagtgg ccaaagccag
     3001 aagcagggtt ataatgggaa cccccccaga tctcattgac cagtcctttt gtgaactgtg
     3061 gggcgcgctt ttggcagaca aagaagtagc tgttccagtc aacgatctac ttgattcgcg
     3121 gggacctgaa gggcagaaga agttcaggga catcttctat gctcccacgt tcgacttgta
     3181 ccaggtgtcg ggcaagtcat atttcgtccc ctatgagttc attacatcag gtttctttta
     3241 tgacaagacg accttcaaga aatatgggtt gcaggaaccg aagacatggg acgatctcat
     3301 ggatgtggct ggaaagctga aatcgaaagg cattgctcca tttgcgcaag atgggaacat
     3361 atcgttctac aacgcctact ggttctattg gttgaccaac aggatacttg gtcccgggtc
     3421 cttttatgct gccgcagctg atcggaccgg gaaaacatgg gatcatccgg ggtatttgaa
     3481 ggcagcggag atgatctacg acataagcaa agcccgaaac gggtatttcc agaaaggcta
     3541 tgatggaagc aattaccctg ctgcacagat gggctgggct cgtcgagagt cggcgatgat
     3601 cgtttgtggc acatggattc ctaacgagac tgccgaagga cgagcggccg actttgacac
     3661 tggatatttc ggattccccg aagtcaaggg cggcaaggga acactccaaa acttcgaggc
     3721 gtacatgata ggctttgcga tccccaaggg cgccaagaac gaggaattgg ccaaggagtt
     3781 catcaagttc acgctcaaga aggaatacca ggagatgata gcggacaagg cccagaatat
     3841 tgcagctcgc aaggacgtga agctgcctgc ggtgctggcg gatgtggaac cgtatcttag
     3901 aaatgctaca gagttccaca tggagtatga cggcgtacag gctaagcttc cggagtggtt
     3961 cacaagcgtg ttctatgggt tagatgacaa gctcttgttc ggaactatca cgccgaatga
     4021 attcatacga caaatcaagc agcaaactat caattattgg cagaagaaga agtagtggtt
     4081 aagtcagttg atattttgtg tgcatgagaa ccggggcaga ccatgggcgc gatcagtgcg
     4141 gagatggttt gcccctgtcc tccacgccgc tgaaaggatg cgcgataatc cagccccaac
     4201 ctggttttag gccaatagac tcgaaagtcg cctttgcgat cgagttgggt tgaggtctga
     4261 ttatgggggc ttatctaagc gaggggtgca tgttgaaacg aaacgacaag acctttgtaa
     4321 tcgcttgtct ggtgccggcg ttagttgtgt atgttgtatt cttcgtattt cccgcattgg
     4381 ccgcgctata tgtgagtttg acagactgga gtggttttac tcccaatctc gagtttatcg
     4441 ggctcaggaa ttttcgcgag ctctttgcag atgagcattt ctggaaagtc gttataagaa
     4501 acactctagg cataatcttc ataggcggag ccattatctt cgcgctggcg ttcctattta
     4561 gcgccatact gagcagcgga atcaaatcta aacggctgct cagaagtata gtatttttcc
     4621 cgaacatcat taaccccatt ggtcttgcgg tcatgtggtc gttcatctac gagcacgact
     4681 cgggcctact taacaacttt ctcagagcta taggcttagg agggcttgcc agaacatggg
     4741 ctgctccaga tcggttgttc tggtctttgc tggtggccct ggtatggata tacgtaggat
     4801 tctttgtggt cattttgatg gcaggtttcg accgaattcc taacgatgtt attgagtccg
     4861 ccaagataga aggagctaat gatgtgcgga tattcttttc cattaaagtc cctatgatat
     4921 gggatgtgct caccatggct atcgtgttgt ggggcatcat ggcccttaag gaattcgcct
     4981 tcctgtattc atggggag
//
LOCUS       DAOSAI010000164         4988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10614_length_4988_cov_4.130347, whole genome shotgun sequence.
ACCESSION   DAOSAI010000164 DAOSAI010000000
VERSION     DAOSAI010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10614_length_4988_cov_4.130347"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="PLM83_06470"
     CDS             complement(<1..1375)
                     /locus_tag="PLM83_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOK71082.1"
                     /translation="MTERSESDDVAISLDISCCGGPAVDDLAWDSGNRNGCDRGAVSE
                     VGSCEMKDDSVQESGGASQGIFSGVDLDEMMSHPSAMRKSILALAWPVVTELVLQTVT
                     QMADMIMVGRLGSQCVAAVGLSNQPFNIIQGCFMGIGVGTTALVARFMGAGDEGRADR
                     VTAQSLAIGSILAAVFAVFGYYFTPNFVRFMKAGPDVVPYAESYMRVLMPGLFMLVIN
                     MIASSALRGAGDTRYPMRVNIVLNLANIVGNYILIYGKFGAPALGVTGAALSTTIART
                     IGTAMLLWHLFSGKGRISVRTQGERFRFDPVLIRRVFAVGLPASLERVSLSLGISFYV
                     RIVSALGTAAYAAHAIAINAEGISYMPGFAFAVAATTMVGQALGADRPDVAERAGIES
                     WKMSATIMGLMGVVLFCFPDALMRLYGDDLEIISMGAEVLRIMAVAQVPMATSYVFVG
                     ALRGAGDT"
     gene            complement(1567..2622)
                     /locus_tag="PLM83_06475"
     CDS             complement(1567..2622)
                     /locus_tag="PLM83_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71083.1"
                     /translation="MKDSKKWTVAALLVTVFLFSSSVANAASITVVEGTPVFFGGLSV
                     MHSEDGPLNLELNWLGIASDLDWEGATLRIGGAICPSSPAFSEGSAAYTFGVGAEMAR
                     VFSLMAAAEGSVMAYLQDCGDGPVMALVGQATGSTARIGLRTNFGVVPSGFDEQPARL
                     IGQSDWSDEPGRDLTGRGWIDVVGALQSSRTRADSWKIGYMRELGESDWRTDIGYIAR
                     YSPEDLGVAIKFYSGLAFGEPRVMLLSKLTFRADVTDSSGAEIDLVVQGGGGSEYAWV
                     SGTGWLSLGDRADLKLRICAPIMSEHEYDGPELFEPIDWAAFAVGLDFNEGIISSVTA
                     RYDLRTKAIGLELASRF"
     gene            complement(2612..>4988)
                     /locus_tag="PLM83_06480"
     CDS             complement(2612..>4988)
                     /locus_tag="PLM83_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOK71084.1"
                     /translation="MRIYKQSGENTVTVARQVREALAQLGQEHRAIEIGYVFDQSTFI
                     TSSIDSLAENAGLGAVLAIAILFLFLRHFGSTLLVAISIPLSMIFAFVMMYAFDLTVN
                     LLTLGGLALGVGMLVDNSIVVLENIFRRQELGESPREAAIQGTAEVSRAITASTLTTI
                     AVFLPVVFIQSMAGHIFRDLAMTVTFSLLASLIVALTVLPLMASSFVRVSDSYIERAK
                     RDRKRKESLGGIYDRMLDWALAKRWWVLGAIGIALVVALFAFPRLGTEFLGSMDMGRV
                     DVGLHLPPGTPVEVTNEVSLRVENEWMKIPGIENVTAEVGSSGSGDFVAMASGGSANQ
                     ASVAVKLLREGETMTSVAEAGAEIREILSDIKLDYPDLEYSVDTSGYGALTGGSSDYL
                     SNSITLEIAGTDPKEISDYAARIAERLRKEPGFVEVASSTKETQPVILLDVNRTRALM
                     GGLTVGQVGLGVRTGMFGSTVTYVERNGELIPVIVRPASDGVPDLDSILNMPITSSLS
                     SASLLEGASGLGSNVSAAARTSSSAISTMLAPSNEVLVGKVVTPEIIDGPMTISRRNG
                     RRYALVQIAFEGMKLSQAGSLALSAAHEVEAPDGCSVELAGVSRVINEAFSDLGLAGW
                     LAVVLVYMVMAIQYESLLYPLIVMFTMPLAGIGAIGFLVLTGQSISITSIIGLIVLAG
                     IVVNNGIVLVDFINQLKAEGMATREAVREAGRARLRPILMTALTTILGLVPLAIGHGA
                     GSELQQPMAITVIGGLTTATFLTLFVIPIVYELMDELAQRLSRKGGTVIEG"
BASE COUNT         1236 a         1579 c         1156 g         1017 t
ORIGIN      
        1 tcgtatctcc agccccgcga agggcgccca caaagacata ggaagtggcc attggcactt
       61 gagctacggc catgatacgc aggacctctg cgcccatact gatgatttcc aggtcgtccc
      121 cgtacagcct catcagggcg tcgggaaagc agaataggac cactcccatg agacccatta
      181 tggtcgcgct catcttccat gactctatgc ctgcccgttc agccacatcg ggcctatccg
      241 ccccaagcgc ctggccaacc attgtggtgg ctgccaccgc aaaggcaaaa ccgggcatgt
      301 aagaaatccc ctcggcatta atagcaatcg cgtgcgctgc gtaggccgca gtgcccagag
      361 cagagactat cctgacatag aaggatattc ccagactcaa gctgacacgt tcaagggagg
      421 ccggcaaacc cacggcaaag accctgcgga taagcaccgg gtcgaaccga aaacgctcac
      481 cctgagtgcg cacagaaatt ctgccttttc ccgaaaacag atgccaaaga agcattgcag
      541 ttccgatcgt tcgtgcgatt gtagtagaca aagctgcacc tgtgacgcca agagcagggg
      601 cgccgaactt cccgtatatg agtatgtaat tccccacaat attggcaaga ttgaggacaa
      661 tattcaccct catggggtac cgcgtatcac cggcgcctcg aagggcagat gaagctatca
      721 tatttatgac aagcatgaat agccccggca tgagaacgcg catgtagctt tcggcataag
      781 ggaccacatc agggcctgcc ttcataaagc gaacaaagtt gggagtgaaa tagtagccga
      841 acacggcaaa gaccgctgca agaatgctcc cgatagccaa cgattgagcg gtaacgcggt
      901 cagcgcggcc ctcgtctccc gctcccataa accgcgccac tagggccgta gtgccgactc
      961 cgatgcccat aaaacacccc tgaattatgt taaacggctg atttgaaagg cctacagcgg
     1021 caacgcattg ggaacccagc ctgccaacca tgatcatgtc cgccatctgg gtaaccgtct
     1081 gcagaacaag ttcggtcact acgggccacg caagggccaa gatcgatttg cgcatagcag
     1141 atggatgcga catcatctcg tctaagtcga ctccggaaaa tatcccctgg gatgcgcccc
     1201 ccgattcctg aaccgaatca tccttcatct cgcatgaccc aacttcgctc actgcgcccc
     1261 tatcacaccc gttcctattg ccgctatccc atgccaggtc atctacggca ggtcccccgc
     1321 aacatgatat atctaaacta atggctacgt cgtcgctttc tgatctctcc gtcatgctta
     1381 gaatcccccg tctgtaattc ttgaccccgg gaccccgaag agcaactctg cctcgccggt
     1441 cccagggcca cactacacgt ttgtaattcg gcggccatga ttttgcatcg ccaagatgaa
     1501 acggaatcga gctgcccact ggcaagccag gcatcccatt gattgccagc cttgtcagcc
     1561 tctagctcag aatctcgatg caagctccag gccgatcgcc ttggttcgca gatcgtatct
     1621 cgcggtcaca ctggagatga tgccctcgtt aaaatccaag cctacagcga aagccgccca
     1681 gtctatcggc tcaaaaagct ctggcccgtc gtactcatgc tcactcatga tcggagcgca
     1741 aatcctcagt ttcagatcag cccggtcgcc aagcgaaagc caacccgtcc cggaaaccca
     1801 agcatattcg gagcctcccc cgccctgtac aacaaggtcg atctccgccc ctgaactgtc
     1861 cgtcacgtcg gccctgaatg tcagcttcga caggagcatc actcgaggtt cgccaaaagc
     1921 cagaccggag tagaatttta tcgcaacgcc aagatcctcc gggctgtatc tcgcaatgta
     1981 cccgatatct gttcgccaat cggattcgcc aagctccctc atatatccaa ttttccagga
     2041 gtcagctctc gttcgcgagc tttgaagggc tccaaccacg tctatccagc ccctacctgt
     2101 cagatccctt ccaggctcat cactccagtc actctgcccg attagacgag cgggctgctc
     2161 gtcaaatccg ctgggcacca cgccgaaatt cgttctcaat cctattcgag ccgtggaccc
     2221 cgttgcctgg ccgaccaacg ccatcactgg cccatcccca caatcctgaa gatatgccat
     2281 aaccgagcct tcggctgcag ccataaggct aaaaacccta gccatctcgg ctcccacgcc
     2341 aaaggtgtag gcggccgatc cttcactaaa tgcaggcgaa ctcggacaga tagccccgcc
     2401 tattcgcagt gtagcgcctt cccaatcgag gtcagacgca attccaagcc agtttagctc
     2461 gagattgagc gggccgtctt cagaatgcat aactgacaaa cccccgaaga aaacaggggt
     2521 cccctctaca acagtgattg aagccgcgtt cgccaccgaa gagctaaaca aaaatacagt
     2581 aacaagtagc gcagcgacgg tccacttctt gctatccttc aattaccgtg ccccctttgc
     2641 ggctcagcct ctgggcaagc tcatccatta gctcgtaaac gatcgggatc acaaatagcg
     2701 tcagaaatgt ggcggttgta agcccgccaa tcaccgttat ggccatcggc tgctgaagct
     2761 cagaacctgc cccatgaccg atggccagcg gcacaaggcc gaggatcgtg gtgagcgccg
     2821 tcatgaggat cggcctcaga cgcgccctgc ccgcctcgcg aaccgcttcg cgtgtcgcca
     2881 ttccctccgc tttgagctgg ttgatgaagt caaccaacac aatgccgttg ttcaccacta
     2941 tgccagcaag tactatcagc ccaatgatcg aggttatact gatgctctgg cccgtgagga
     3001 caaggaatcc aatggcccct atacccgcca aaggcatcgt aaacatgact atcaagggat
     3061 aaagcagaga ctcatattgt atagccatca ccatgtaaac aaggaccact gcgagccagc
     3121 ctgcgaggcc caaatcggag aatgcctcgt ttatgacacg gctcacaccc gcaagttcaa
     3181 cagaacagcc gtccggcgcc tccacttcgt gggctgcaga cagagccaag cttcccgcct
     3241 gtgatagctt cattccttca aacgcgattt gcacaagggc ataacgcctt ccattgcgcc
     3301 tgcttatcgt catcgggcca tcaattatct caggcgtcac aaccttgccc accagaacct
     3361 cgttggatgg cgccagcatt gttgatatag cactcgaact ggttcgggct gctgctgaaa
     3421 cgttgcttcc cagcccgctc gcgccctcaa gcaaacttgc tgagctcaac gaagacgtaa
     3481 tcggcatgtt caaaatactg tcaagatcag gcactccgtc agatgccggc ctcacaatta
     3541 cgggaatgag ctccccattt cgctccacgt atgtcaccgt agatccgaac atcccggtgc
     3601 gaaccccgag cccaacctgg cccacggtaa gccctcccat gagcgccctc gtacggttta
     3661 catcgagcaa gataacaggc tgggtttcct ttgtcgacga agcaacctca acgaatccgg
     3721 gttctttacg caatctttcg gcgattctgg ccgcgtaatc cgagatctcc tttggatctg
     3781 tcccggctat ctccaacgtt atactattgc tcaggtaatc tgaactgcca cccgtcaaag
     3841 cgccatatcc cgaagtgtcc actgaatact cgagatcagg gtagtccaac ttgatgtcgc
     3901 tgaggatctc cctaatctcg gccccagcct ctgcaaccga ggtcatcgtt tctccttcac
     3961 gtaagagctt taccgccacc gacgcttggt tagcgcttcc gccgctagcc atggccacaa
     4021 aatccccgct gccggaagaa cccacttcag cagtgacatt ctcgatcccc ggaatcttca
     4081 tccattcgtt ctcgactctt agcgagacct cgttagtaac ctctaccgga gtccctggag
     4141 gaagatgaag tccgacatcg actcttccca tatccattga ccctaagaat tcggtcccaa
     4201 gcctgggaaa agcgaagagc gccacaacga gggcgatccc aatagcgccc aacacccacc
     4261 atctcttcgc aagcgcccaa tcgagcatgc gatcgtatat tccacccaaa gactccttgc
     4321 gcttcctatc ccgtttcgcg cgttcaatat aggaatcgga cacgcgcacg aacgaacttg
     4381 ccatcaaggg caagaccgta agggctacta tgagcgacgc caacaaagaa aaggtcaccg
     4441 tcatggcgag atctctgaaa atgtgccctg ccatgctctg gatgaacaca acaggaagaa
     4501 agaccgcaat cgtggtcaaa gtagaagccg taatagctct cgaaacctct gctgtgcctt
     4561 gaatcgcggc ctcgcgcggc gactctccca gttcttgccg ccgaaagata ttctccaaca
     4621 caacgatgga gttatctact aacattccaa cacccagagc cagtccgccc aaggtgagga
     4681 ggtttaccgt gagatcaaag gcgtacatca tcacgaacgc gaatatcatc gaaagcggaa
     4741 tggagatcgc taccaagagc gtgctcccga agtgtcgcaa aaacagaaac aaaatcgcga
     4801 tagcgagaac cgccccaaga ccggcgtttt cagcaaggct gtctatagag ctcgtaataa
     4861 acgtggactg gtcgaacaca tagcctatct cgatggcccg atgctcctgc ccgagctggg
     4921 ctaatgcctc cctcacctgc ctggccactg tcaccgtatt ctcgcccgac tgcttgtaga
     4981 tcctcatt
//
LOCUS       DAOSAI010000165         4988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10615_length_4988_cov_3.288668, whole genome shotgun sequence.
ACCESSION   DAOSAI010000165 DAOSAI010000000
VERSION     DAOSAI010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10615_length_4988_cov_3.288668"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..945
                     /locus_tag="PLM83_06485"
     CDS             31..945
                     /locus_tag="PLM83_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK71085.1"
                     /translation="MLKTVYNINMVGPPGAGKTLMASCVPSVLPAMDREEAIEVTSIQ
                     SIMGLVDGRGLAVSRPFRNPHHSITPAGMLGGGNPPMPGEVTLAHRGVLYLDEYCEYR
                     QAVLQCLRSPMEDGFVVISRSHVQVAFPSRFMLVASANPCPCGYLGDSERPCVCSGAQ
                     VQRYRRKLSGPVIDRIDIQIEAPRVSRADLQGHSAGRSSDKIRANVSEARERQRRRFG
                     NPIVTNSCMDPRNIEHAAMLSPIAGALLYEAVDKMALSARACHKLIKVARTIADLDAS
                     ARIKDDHVLEALQYRALDSAEIARCSHG"
     gene            938..2086
                     /locus_tag="PLM83_06490"
     CDS             938..2086
                     /locus_tag="PLM83_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HOK71086.1"
                     /translation="MDDKEPKLRWENVAEPEGKELAAWVFWNSVPGFGSRSFLRMLED
                     YGSAVEGLHAAKHGGKPPAYARRAQWNWAGRVHDPLCMGMTEIAKASSISARVIPCPS
                     RLYPAPLRNIPDPPIVLYVLGKGELTEVPWAAMVGSRQATAYGISIARRYGRMLASAG
                     IGVVSGLARGIDAAAHRGAIEGGGPTVGVLGAGMGDDMTGRLKKLIREVAACGCVISP
                     FPMGWAASKDTFPARNRIISGMSEVCIVVEAAEKSGAKITADFANEHGKDVFAVPGDI
                     NRPTSVGANQLLSTHAEPLLDIGDVIDRIASRGLLARTHGPTARISGGVLAPVDRAVL
                     ESLTVNTSFERIQTDLGLPAQELLVSLARLEFAGLVDREPGMRYSKRC"
     gene            2200..4326
                     /gene="topA"
                     /locus_tag="PLM83_06495"
     CDS             2200..4326
                     /gene="topA"
                     /locus_tag="PLM83_06495"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOK71087.1"
                     /translation="MGKTLVIVESPAKVKTLSKFLGRQYALKASVGHVRDLPKSQLGV
                     DVDNDYAPKYITIRGKGDVIRDLKESAKKADRILLATDPDREGEAISWHLAYILGVDP
                     ESDCRIEFNEITENAVKNAIKAPRPIDMGRVEAQQARRVLDRLVGYKLSPFLRKKVRP
                     GLSAGRVQSSALKIICDREREIEAFESEEYWTLGAELSPKPRGRSFEARFIPEEDKRL
                     SKEEVDAVIADLEGADYVVESINVRDQARKPAPPFITSTLQQEAFRKLGFSGKRTMSN
                     AQALYEGVEVAGEGHVALITYMRTDSTRVAQEAQAAARDYVRKTWGAKYVPKSPRVYA
                     SRKGAQDAHEAIRPTSVERTPESLKGSLGRDQLRLYTLIWSRFMASQMSDAVNEITSV
                     DISAGEHRFRATGQKVKFDGFTVIYTEGRDDESSKNNDKRQLPALEVGQRLDLVQLLP
                     EQHFTQPPPRYTEATLIKELEKHGIGRPSTYAPIIDTLRKRDYATLDKKRFIPTEVGF
                     AVCDLLSEYFPSVVDLEFTAKMEEKLDSIEENEADWVSVTDEFYKPFAQKLAEAEELA
                     EKVVIEDEPIGEACPECGAELVIKSGRYGKFIACPMYPKCTYRRSIVKKLDVKCPKCG
                     GDVVERRSKKGRTFYGCSNYPNCDFVAWNRPSKVSCPMCGSFTTVRQRKDEMIYKCAD
                     AECGHTWKEAADSGSGAQQGGTGVAE"
     gene            4316..>4988
                     /gene="trmFO"
                     /locus_tag="PLM83_06500"
     CDS             4316..>4988
                     /gene="trmFO"
                     /locus_tag="PLM83_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOK71088.1"
                     /translation="MQNEVIVIGAGLAGSEAAWQASQMGADVKLYEMRPIVSTPAHST
                     GNFAELVCSNSLGSDREGTAPGLLKEELRALGSIIMRCADRTRVPAGQALAVDRGLFS
                     ALVTKELEAAPNIEVIREEVVRVPREGVVVIATGPLTSPAFSEEIASLLGTDHLYFFD
                     AMAPIISGHSVNWSRAYWASRYGRGDSDYANCAMNREEYELFWGNLIEAETAEMHEFE
                     SLQLFE"
BASE COUNT         1145 a         1207 c         1540 g         1096 t
ORIGIN      
        1 cagaactgag cgaatagcat aaaaccgttt atgcttaaaa ccgtttataa tattaacatg
       61 gttggcccgc ctggtgccgg caagacgctg atggcgtcgt gcgttccatc tgtgctgcca
      121 gcgatggatc gcgaggaggc aatagaggtc acgtcgatac agagcatcat gggcttggtg
      181 gatgggcggg gccttgccgt cagccggcct tttaggaatc cccaccattc gattactccc
      241 gcagggatgc tgggaggagg gaatcctcca atgcccggag aggttacatt ggcgcacaga
      301 ggtgtgcttt acctggatga atactgtgag taccgccagg cagttctcca atgcctgcgg
      361 agtcctatgg aggatggatt cgttgttatc tcccgttctc acgtacaggt tgcttttcct
      421 tctaggttca tgctggttgc gtcggccaat ccatgccctt gcgggtatct tggggattca
      481 gagcgtccgt gcgtgtgtag cggggcacag gttcaaaggt acagaaggaa gttgtcgggt
      541 cctgtcattg ataggatcga tattcagata gaggcacccc gtgtgagccg cgccgatctg
      601 caagggcatt ctgcgggccg ctcgtctgac aagatccgcg ccaatgtgag cgaagctagg
      661 gaaaggcaga ggcggcgctt tgggaacccc attgtaacaa attcgtgcat ggaccctcgc
      721 aatatcgaac acgcagcaat gctctcgcct attgccgggg ctttgctgta tgaagccgtt
      781 gacaagatgg ccctttcagc aagggcctgc cacaagctta taaaggtggc cagaaccatc
      841 gcggatttgg acgcttctgc ccggatcaaa gacgatcacg tgcttgaagc gcttcaatac
      901 agggcgctcg attcggcaga gatagcgagg tgttcacatg gatgataagg agcctaagct
      961 gagatgggag aatgtagctg agcctgaggg aaaggaactt gctgcgtggg ttttctggaa
     1021 ttcggtcccg ggttttgggt cgcggagctt tctgcgcatg ctggaggact acggatcggc
     1081 cgtagagggc cttcatgccg caaaacatgg cgggaaaccg cctgcttatg cccggcgcgc
     1141 ccaatggaat tgggcgggaa gggttcacga tcctctctgc atggggatga ccgagatagc
     1201 caaggcctcg agtatatcgg cgcgtgtgat tccctgcccg tcccggctgt atcctgcgcc
     1261 attgcgcaat attcctgatc cgcccatcgt cctgtatgtg ttggggaaag gggaattgac
     1321 ggaggttccc tgggctgcaa tggttggctc caggcaagct actgcttatg gcatctcgat
     1381 tgccagaaga tacgggcgca tgcttgcgtc tgccggcatt ggtgtagtaa gcgggcttgc
     1441 ccggggaata gatgccgctg cccaccgtgg tgctatcgag ggaggaggac ccacggtagg
     1501 ggttctcggc gcgggaatgg gcgatgatat gacagggagg ctcaaaaagc tgatacggga
     1561 ggtggccgca tgcggatgtg taatatcgcc ttttcctatg ggatgggcgg ctagcaagga
     1621 cacttttccc gcccgaaata ggatcataag cggcatgtcg gaggtgtgca tagtggtgga
     1681 ggcggccgag aagagcggtg ccaagatcac cgcggacttc gccaatgagc atgggaagga
     1741 tgtattcgct gtccccggcg atatcaacag gccgacctct gtaggagcta accagctgct
     1801 ttcgactcat gccgaaccat tgctcgacat tggcgacgtc attgatcgga ttgcatcaag
     1861 gggtcttctt gccagaaccc atggcccgac ggctcgtatt tcaggaggcg ttcttgcacc
     1921 tgtggatcgc gctgtgctgg aatcattgac tgtgaacaca agttttgaga gaattcagac
     1981 agacttggga ttgcccgctc aggagctttt ggtctctttg gcgcggctgg aattcgcggg
     2041 cctggtagac cgggaacctg gcatgcgata cagcaaacgg tgctagaaaa cgtgctgtgc
     2101 ccggcgcctg atgtggcatg ctccagggct tctcgatgtt gacctggcat tctgcctagg
     2161 ttaaactgtt tttgatatcc agactttgga ggtgcccgta tgggaaagac tctcgtaata
     2221 gtggagtcgc cagctaaagt aaagacgtta tcaaaattcc ttgggcgcca gtatgcactg
     2281 aaggcgtcag ttggtcacgt gagagacttg cctaagagcc agttgggagt ggatgtcgat
     2341 aatgactacg ctccaaaata cattacgatc cgcggcaaag gcgatgtgat acgcgacctg
     2401 aaggagtccg cgaaaaaggc agacaggata ctcttggcca cagaccctga tcgtgaggga
     2461 gaggctattt cgtggcatct agcctacata cttggcgtgg atccggaatc agattgccgg
     2521 atcgagttta acgagattac tgagaacgct gtcaagaacg cgataaaggc cccacgccca
     2581 atcgacatgg gcagagttga ggcgcagcag gctaggcgcg tgcttgacag gcttgttgga
     2641 tacaagctca gccctttcct tcggaagaag gttcgccctg ggctttcggc cggtcgcgtt
     2701 cagtcatctg ctctgaagat aatatgtgat cgcgagcgcg agatagaggc cttcgagtca
     2761 gaggagtact ggactctcgg tgccgagctt tctccaaagc ccagaggccg cagctttgag
     2821 gcgaggttca ttcccgaaga agacaagcgt ctttcgaagg aagaggtaga tgcggtcatt
     2881 gctgatttgg agggagccga ttacgtagta gagtcgataa acgttagaga ccaggccaga
     2941 aagccggcgc ctcccttcat cacaagcacg ctgcagcaag aggcgttcag aaaactgggg
     3001 ttttccggca aacgcactat gtcaaatgcc caggcgcttt atgaaggcgt cgaagttgcc
     3061 ggagaagggc atgtggcgct catcacgtac atgcgcacag attccaccag ggtagcccag
     3121 gaggcgcagg cagctgccag agattacgtg cgcaagacgt ggggagcgaa gtatgttccg
     3181 aaatctccga gagtttatgc ctccagaaaa ggggcacagg atgcccacga ggctatcagg
     3241 cctacgtccg tggagcgcac gccagaaagc ctcaagggaa gcctaggccg tgaccagctc
     3301 aggctgtata ctctcatctg gtcaaggttc atggccagcc agatgtcgga tgctgttaac
     3361 gagatcacgt cagtcgacat atctgcgggc gagcacagat tccgcgcaac aggccagaag
     3421 gtgaagttcg acggattcac tgtgatctac acggagggcc gtgatgatga aagttctaag
     3481 aacaatgaca agcgccagct tcccgccctc gaagtcgggc agcggctcga tctggtgcag
     3541 ctcctcccag agcagcattt tacgcagcct ccgccacggt atactgaggc cactttgatc
     3601 aaggaactgg aaaaacacgg cattgggcgc ccaagcactt atgcgccgat aattgacact
     3661 ttgcgaaaaa gggactacgc gacgcttgac aagaaacgtt tcatcccaac tgaggtcggg
     3721 tttgctgttt gcgatctctt gagtgagtat tttccttctg tggtggactt ggagtttaca
     3781 gcaaagatgg aggaaaagct agacagcata gaggagaacg aagctgattg ggtcagcgtc
     3841 actgacgagt tctataagcc atttgcgcag aagcttgccg aagctgaaga actcgccgag
     3901 aaggttgtga tcgaagacga gcctatcggc gaggcatgcc ccgaatgcgg cgccgagcta
     3961 gtcataaaaa gcggacgtta tggcaagttc attgcttgcc ccatgtatcc taaatgcacg
     4021 tataggcgga gcatagtgaa gaagctggac gtgaagtgcc cgaaatgcgg cggggatgta
     4081 gtcgaacggc ggagcaagaa gggacgaact ttctatggtt gttccaacta tccaaattgc
     4141 gatttcgtgg catggaatcg tccgtcgaag gttagttgcc cgatgtgcgg ctcgtttacc
     4201 actgtgaggc agaggaagga cgagatgatc tacaaatgcg ctgatgccga atgtgggcat
     4261 acatggaagg aggccgcaga ctccggcagt ggcgcacaac agggagggac cggggttgca
     4321 gaatgaggtt atcgttatcg gcgccggcct tgcagggagt gaagccgctt ggcaggcttc
     4381 gcagatgggc gcggatgtca agttgtatga gatgcgccca atcgtgtcaa ctcctgcaca
     4441 ttccactggc aatttcgccg aactcgtctg cagcaactct ctcgggtctg atcgagaggg
     4501 gaccgctccc ggattgctca aagaggagct tcgggcatta ggctccatta taatgcgttg
     4561 cgccgacagg acacgtgttc ctgcaggaca ggctctcgcg gttgacaggg ggcttttcag
     4621 tgccctagtt acaaaggagc tcgaggcggc gccgaatata gaggtgatcc gggaggaagt
     4681 tgtcagggtg cctcgggagg gcgtggtagt tattgccact ggccccctca cgtccccggc
     4741 tttctcggaa gaaatcgcat cgttgctcgg caccgaccac ctttactttt ttgacgctat
     4801 ggctcccata ataagcggtc attctgtaaa ttggtcacgc gcatattggg catcaagata
     4861 cggcaggggc gattcggatt acgcgaactg tgccatgaac agggaggaat atgagctttt
     4921 ttggggaaat ttgattgaag ctgagacagc agagatgcat gagttcgaat ctctccagct
     4981 gtttgagg
//
LOCUS       DAOSAI010000166         4967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10680_length_4967_cov_4.289699, whole genome shotgun sequence.
ACCESSION   DAOSAI010000166 DAOSAI010000000
VERSION     DAOSAI010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10680_length_4967_cov_4.289699"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1168
                     /locus_tag="PLM83_06505"
     CDS             38..1168
                     /locus_tag="PLM83_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOK71089.1"
                     /translation="MQEAVVEFLYSTGFAVLTWKELVMFAIAGCLMYLAIVKKFEPLL
                     LLPISFGMLLANLPAAGLMEGPQDGIVGGLLYYLYQGVKLGIYPPLIFLGVGAMTDFG
                     PLLANPSTIFLGAAAQFGIFITFLGALLLGFSPAQAGSIGIIGGADGPTAIFVTSRLA
                     PELLGPIAIAAYSYMALVPIIQPPIMRALTRKEDRQIVMEQLRPVSKKEKILFPIIVT
                     VVVGLMLPSAAALVGMLMLGNLFRESGVVDRLSKTAQNELINIVTIFLGLTVGATASA
                     ESFLTWQTISIILLGLVAFSGGTAGGVLLGNLMCRLTKGKVNPLIGAAGVSAVPMAAR
                     VVQTVAQDENPGNFLLMHAMGPNVAGVIGSAIVAGVLLSRFG"
     gene            complement(1155..1643)
                     /locus_tag="PLM83_06510"
     CDS             complement(1155..1643)
                     /locus_tag="PLM83_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71090.1"
                     /translation="MLHDFYTFVENIAKTVAMRIDGHDWHRELLEQMNPRTLSAALVQ
                     RHSCAPRQVPYVFRNIYGFQPDADRTLELVCDMHKTVSALISDLDCHVREIDRITRWK
                     PEEPLATSGERREHPPSVWPCIGREERGMTSSVCPSEHWHMPVRLSNHLLITDVNSSI
                     QT"
     gene            complement(1701..2129)
                     /locus_tag="PLM83_06515"
     CDS             complement(1701..2129)
                     /locus_tag="PLM83_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71091.1"
                     /translation="MGVSGVLPQFAPQLVDELQLLLGGVTMVDDGIHGWNVPEIREAV
                     FWWSEERAELVMADAMERARRIADLLGTRYSAPAVTLYGSLAEGCILVEGFRGDYWEM
                     YVMADEIADPLSMRGKGGSAFQTRGLPGDSGPNSQGTRES"
     gene            complement(2494..3201)
                     /locus_tag="PLM83_06520"
     CDS             complement(2494..3201)
                     /locus_tag="PLM83_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71092.1"
                     /translation="MQAKRLRKLTVSMIVAVTLVVVTAGACSAQVGGGGNSVGWVFLR
                     VDDLNESLVDAEFNQLPGGMLTWGGYGTVGVGNGKWSIGGWYGGGEAISKPTAGAEPK
                     IATLALNYGGILVEYSAVDSPKAHVGLGTVLGGGMASLTTKRGMISDFDEALGAASSA
                     SVWRPYLLAQPQASIAFPLSALIDITITGGYSLLYSPTGWMDGMTFKDSFDGPMKSAG
                     LPFVQLSFSFGPPPLGI"
     gene            complement(3217..3618)
                     /gene="mce"
                     /locus_tag="PLM83_06525"
     CDS             complement(3217..3618)
                     /gene="mce"
                     /locus_tag="PLM83_06525"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOK71093.1"
                     /translation="MVKSVDHIGIAVADLEEALKLYTETFGLELQGTEVVEEQKVKVA
                     FLPIGDTEIELLESTDPEGPIAKFIEKRGQGIQHIALRVDDIDAALDKMRAEGIRLID
                     EKPRYGAGGARIAFLHPKATGGVLIELCERK"
     gene            complement(3639..4703)
                     /gene="meaB"
                     /locus_tag="PLM83_06530"
     CDS             complement(3639..4703)
                     /gene="meaB"
                     /locus_tag="PLM83_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOK71094.1"
                     /translation="MGRTEVVSRALSGDIRAVSRLISLIEDCVPGFEGLYSEIYPFTG
                     KAHVIGITGPPGSGKSTLVDKMAAVCRERGKTVGIVAVDPTSPFTGGALLGDRLRMQR
                     HALDDGVFIRSMGTRGQLGGISRATSGAVDVLDAAGYDYVIIETVGVGQSEVEIASIA
                     DTVVLVQVPGLGDDIQVIKAGVMEIGDLFAVNKKDRPGVERVVAEIRIALELSSVASR
                     GGGGTSRIAGSAGHHGVSALGAAGSEREETVDEEAARPCWHPPILQTLAETGEGVGEL
                     FDACDEHMRYLAGTGELDDRRYRRARSETMQIASGRIWRGLTDDRGAFAAAEELAWKV
                     ARREIDPYSAGNELYEMIVK"
     gene            complement(4706..>4967)
                     /locus_tag="PLM83_06535"
     CDS             complement(4706..>4967)
                     /locus_tag="PLM83_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOK71095.1"
                     /translation="VAAAIQEDVDVIAMSILSGAHNVLFPKVMDLLREKGVDDILVVG
                     GGIIPDEDIPALKECGIADIFTPGTSTSETVKFIEENVKRRS"
BASE COUNT         1095 a         1551 c         1217 g         1104 t
ORIGIN      
        1 gtatccatag acccggccta aaggagggcg actttcagtg caagaagcag tcgtcgagtt
       61 cctatacagt acgggctttg ctgtcctgac atggaaagaa ctcgtcatgt tcgctatcgc
      121 gggctgcctc atgtacctgg cgatagtaaa gaagtttgag ccgctgctcc ttcttccgat
      181 ctcttttgga atgctcttag caaacctgcc ggctgctggg ctcatggagg gtccgcaaga
      241 cggcattgtt ggaggattgc tctactatct ttaccagggc gtcaagttag gcatataccc
      301 gccactcata tttctgggcg taggcgcaat gaccgacttc ggtcccctgc ttgccaatcc
      361 gtctacgatc ttcctgggag ccgccgctca gttcggaata ttcatcacgt ttctcggcgc
      421 actgctccta gggttttcgc cagcgcaagc ggggtcgatc ggcatcatcg gcggcgcaga
      481 cggcccaaca gctatattcg tcacgagcag gctagcaccg gaacttctgg gcccgatagc
      541 aatagctgcc tactcctaca tggccttagt gccgataatc cagcctccta ttatgcgtgc
      601 ccttacacgc aaagaggacc gtcaaatcgt gatggagcag cttcgtcccg tatccaagaa
      661 ggagaagata ctcttcccaa tcatagtgac tgtcgttgta ggactgatgc tgccatcggc
      721 tgcagccttg gtgggaatgc tcatgctggg caacctgttc cgcgaatccg gcgttgtaga
      781 caggctttca aagacggccc agaacgagct gatcaacata gtcaccatct tcttggggct
      841 cactgtagga gcaacagcca gtgccgaaag ctttttgaca tggcagacta taagcataat
      901 tctcttgggg cttgtggcgt tctcaggggg cacagcaggc ggagtgttgc tgggaaacct
      961 tatgtgccga cttactaagg gcaaggtcaa cccgcttatc ggagcagccg gcgtatcagc
     1021 cgttcccatg gcggcccgcg ttgtgcagac agtggcccaa gatgaaaacc caggcaactt
     1081 cctgctaatg cacgccatgg ggcccaacgt agcaggagtc ataggttctg caatagtcgc
     1141 gggagttctg ctgtctaggt ttggatagac gagttgacat ccgtgatgag caggtgattg
     1201 gataatcgca ccggcatgtg ccaatgttca gatgggcaga cagaactcgt catcccccgt
     1261 tcctcccggc caatacaagg ccagacggac gggggatgtt ctcttctctc tccggaggtg
     1321 gccaaaggct cttctggctt ccaccgtgtt atgcggtcga tctcacgtac gtgacagtcc
     1381 aggtcagaga tgagcgccga cacagtcttg tgcatatcgc agaccaactc tagcgtccga
     1441 tctgcatccg gttgaaatcc gtagatgttc ctgaacacgt acggaacctg tcgaggagcg
     1501 cagctgtgcc gctggacaag agcggcggac agagtccggg gattcatctg ctccagcaac
     1561 tctctgtgcc agtcatgccc atcaatcctc atagccacgg tcttggcgat gttctccacg
     1621 aacgtgtaga aatcgtggag catcgatccg agtatgcgca atgcctacaa gcggaaaccg
     1681 ccacaggata gatgaggtga ctaagattct ctcgttcctt ggctattcgg gcctgaatca
     1741 ccaggtagtc ctctggtctg aaatgcagat ccccctttcc cgcgcatgct caaagggtcg
     1801 gctatctcgt cagccatgac gtacatttcc cagtaatcgc ccctgaaccc ttcaactagg
     1861 atgcaacctt ctgcgaggga tccatacagc gtgaccgcag gggccgaata gcgagttcct
     1921 agaagatcag caatacgacg cgccctttcc attgcatcag ccataacaag ctcggcgcgt
     1981 tcctccgacc accaaaatac cgcctccctt atttcaggca cattccatcc atgaatgccg
     2041 tcatcgacca tggtcacacc ccccaacaaa agctgtagtt catccacaag ctgtggcgca
     2101 aactgcggaa gcactccact cacgcccact cagccaaatc cgtgctcccg caagcaggtt
     2161 gtccccacca tcgaagcaat ttctgcatcg aaaagcggcc tcgcagagat tacgcccacg
     2221 tatcctgcga cgagatacgt aacgttgccg ctactatgcc aggtttgtgg gagaacatcg
     2281 ctttgcccaa atcaccgcag cctcaggcat ttgcgaatgc gagcagctgc agcaacttgc
     2341 cccgctcccc tttacactct ggaaggctag atccatccac ccgagcgcct ttcgcaaaaa
     2401 agcaagtccc ctgcctcgtg atgcatcacc agggcagggg actgcaactg ctccagccgc
     2461 gctcgattca cttggaaatc gcaggctttg ccgctatatt cccagaggcg gcggcccgaa
     2521 gctgaaggat agctgcacaa agggcagtcc cgcgctcttc atagggccat cgaatgaatc
     2581 cttgaaggtc atcccatcca tccatccggt cgggctgtac aagagagagt atcctccagt
     2641 gatcgtaata tcgattagcg ccgacagcgg gaaagctatg gatgcctggg gctgcgccag
     2701 aagataaggc cgccagaccg aagcagagct ggccgcgccc agcgcctcgt caaagtcgga
     2761 gatcatgccg cgctttgtgg taagcgacgc cattccccct ccaagcactg tacctaatcc
     2821 gacatgggct ttcggagaat ccacggcact gtattcaacc aagattcccc cgtaattgag
     2881 cgcaagagta gctatcttcg gctctgctcc ggcagtaggc ttcgaaatgg cctcgcctcc
     2941 accataccat ccgcctatgg accattttcc gtttccgaca ccaactgttc catagccgcc
     3001 ccacgtgagc atgccgccgg gaagctggtt gaattcggca tcaaccagag attcattgag
     3061 atcatcaacc ctgaggaaaa cccatccgac tgagttccca ccgccgccca cctgcgcaga
     3121 acaggcaccc gcagtcacaa cgaccagggt tacggcaaca atcatagata cggtcagttt
     3181 tcgaagcctc tttgcttgca tagcgtctac ctcactttac ttgcgttcgc agagctctat
     3241 aaggactcca cctgtagctt tcggatgcag gaaagcgatc cgcgctccgc ctgctccata
     3301 gcgaggcttc tcgtcgatga gcctgatccc ttcagcccgc atcttatcga gcgcagcgtc
     3361 gatgtcgtcc actctcaagg ctatgtgctg aattccctgt ccccgttttt ctatgaactt
     3421 ggcgatcgga ccctcaggat cggtcgactc aagaagttct atctccgtat cgcctatagg
     3481 tagaaaagca actttgacct tctgctcttc caccacttca gttccttgga gctcaagtcc
     3541 gaacgtttcg gtatacagtt tgagggcctc ttcaagatct gccaccgcaa ttcctatatg
     3601 gtccaccgat ttgaccaaat cagtcccctc ccgcgaatct atttgacgat catctcataa
     3661 agctcattgc ctgctgaata agggtcgatc tcccttcggg caaccttcca cgccagttcc
     3721 tctgcagcag cgaatgcacc gcgatcgtct gtgagcccgc gccaaatcct tcccgatgca
     3781 atctgcatcg tctccgaacg tgcacgcctg tatctccgat cgtccaactc accggttccg
     3841 gccagataac gcatatgctc atcgcaggcg tcgaaaagtt cgcctacccc ttcacccgtc
     3901 tcagccaatg tctggaggat cggaggatgc caacagggcc tcgctgcctc ctcgtccact
     3961 gtctcttctc tttcagagcc cgccgctccg agcgccgaaa ctccgtgatg ccccgcggat
     4021 ccggcgattc tgctcgttcc gccgcctccc ctagacgcaa cggaactgag ctccagagct
     4081 atccgaatct cagctaccac tctttccaca cccgggcggt cttttttgtt cacggcgaac
     4141 agatcaccga tctccattac gccagccttt atgacctgaa tatcatcgcc caacccaggc
     4201 acttgcacca gcacgacggt gtcagcgatc gaggctatct ccacctcgga ttggcccaca
     4261 cccacagtct cgatgattac gtaatcatac ccggcagcat ccagcacgtc caccgcgcca
     4321 gacgtagcac gcgatatacc gccaagctgg cccctggtgc ccatgcttct gatgaacact
     4381 ccatcatcta aggcatggcg ctgcattcga agacgatcgc ccaaaagcgc cccgcctgtg
     4441 aacgggctag tagggtcgac cgccactatt cccacagttt ttccgcgttc cctgcacacc
     4501 gccgccatct tgtcgacgag tgtgctcttt ccggatccgg gcggaccggt aatccctatt
     4561 acgtgcgcct tccctgtaaa cgggtagatc tccgaataca ggccctcgaa tcctggaaca
     4621 caatcttcaa tgagggatat gagccgggac acggcccgga tatccccgct aagggcccgg
     4681 ctcaccactt ccgttcggcc catgcttacg accgcctctt cacgttctct tctatgaact
     4741 taaccgtttc cgatgtactt gtgccaggag tgaatatgtc cgctatgcca cattctttga
     4801 gggcagggat gtcctcatcc gggatgatgc ccccgcccac gacgagaata tcgtcgacac
     4861 ccttctcacg cagcaggtcc atgaccttcg ggaaaaggac attgtgtgca cccgaaagta
     4921 tcgacatggc gatcacatcg acatcttcct gaatagcagc cgccacg
//
LOCUS       DAOSAI010000167         4966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10681_length_4966_cov_4.156180, whole genome shotgun sequence.
ACCESSION   DAOSAI010000167 DAOSAI010000000
VERSION     DAOSAI010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10681_length_4966_cov_4.156180"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLM83_06540"
     CDS             <1..483
                     /locus_tag="PLM83_06540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK71096.1"
                     /translation="RQSDFHPSASGSEEWRNLMREHYAGYGQCGAFVERAVDLAIHRL
                     AAAWGEGRDYRVFLAFAGDDSDASGVCEIFRHKGVAKIERLYVRKNSRMQGIGSSMIM
                     HAVDYALRAGDDLAYLVADSLDWPRRMYRKLGFEDLMEIVEWTRIWGGSADDEGYVTW
                     "
     gene            792..1013
                     /locus_tag="PLM83_06545"
     CDS             792..1013
                     /locus_tag="PLM83_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOK71097.1"
                     /translation="MREKVEALLDEIRPSLQADGGDVELVDVTEDGIVKVRLKGACAG
                     CPMSTLTIKNGIERVLKNEIPEVKAVQSV"
     gene            complement(1085..2308)
                     /locus_tag="PLM83_06550"
     CDS             complement(1085..2308)
                     /locus_tag="PLM83_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOK71098.1"
                     /translation="MAALVLTGIICAVVFSKGYSPQTPETPNGFRSEPANAEESAVWP
                     GSRKLSQDHESAAGAVVPIDDGGGASPESESEPRSATLCECGDLHKLWLCEPRMVGKQ
                     VRDLQEGLAVAGFPAGHVDGLFGPATEGAVRAFQASVGLSEDGIVDLSVWMALGRVCE
                     QIVAASLEPSGDIIPIAKTTDEQIGAGPEAKSKAKASPAKPQTEQEGTTGKGLPPGWR
                     ALLIDTSNLTITLLEDGLPIKQYRVGVGEPSTPTPLGQFKVIEKAAWAGGFGTRWMGL
                     NVPWGKYGIHGTNKPWTVGQRKSGGCIRMLNRDVEELYSMVPVGTPVVITAGHFGTLG
                     SFRPRIEPGAKGSYVLEVQKRLAKLGYEHAGFDGVYGPGTERAIMQLQRDKGLPVTGI
                     VDMRTYEALGLFIFD"
     gene            2518..3558
                     /gene="tdh"
                     /locus_tag="PLM83_06555"
     CDS             2518..3558
                     /gene="tdh"
                     /locus_tag="PLM83_06555"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOK71099.1"
                     /translation="MRGEMRAISKMRPEPGAELITAPIPQPGRGEVLVRVRATAICGT
                     DVHIYEWDPWSQARVTTPRIFGHEFCGDVVEVGEGVTKVKEGQFVSVETHTACGKCFY
                     CLTGLAHICQNAKIMGIDRDGCFAEYVVVPEFNCWIWDIDISDEVAAIQDPFGNAVHT
                     TLATDLVGKTVLITGMGPIGLCAISVAKAAGATEVYVTDVSDYRLDLACKLGADLAIN
                     PKREDPVAAVREATGGVGADVLLEMSGSPSAIVQGFDALRKAGFASLLGIPSEPIKLD
                     LANAIIFKSATVQGINGRRMFETWFQARSLLKAGLDITPVITHKMSLWDDLDEGMELM
                     MSGQCGKVILYP"
     gene            3678..4550
                     /locus_tag="PLM83_06560"
     CDS             3678..4550
                     /locus_tag="PLM83_06560"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOK71100.1"
                     /translation="MPEVIYSEEKLKAQDGVELFCRVWKGVSLEGEPALKARSTIVIA
                     HGLADHSGRYEHVAKFFAQKGYVVYAYDHRGQGKSEGVRGHVNKFSQFYDDLWFVIRR
                     AKGRNPGKKCFLVGHSMGGLIALGYAARRPKTINGLIVTSPCLALRMRVSPIKKGFGT
                     FISWLFPKMAVDTGIAAEGLSHDSNVVEAYKKDPIRYPKITTKFYVEFDKAMKNVQSL
                     AKSIVTPCLFMPGGDDPICSTEATIKFYENVPETTDKKLIVWDGLYHEIMNEPEKDKV
                     LDTMAQWIAEREGK"
     gene            4550..>4966
                     /locus_tag="PLM83_06565"
     CDS             4550..>4966
                     /locus_tag="PLM83_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOK71101.1"
                     /translation="MPALSSAERQRYSRQIRLARVGEVGQGRIRAARVLIVGVGGLGS
                     PIALYLAAAGVGVIGLVDPDVVELSNLQRQIAHTTCDIGRLKVDSAERRILALNSEVQ
                     VEKWAVALDIDNAGDLVGRYDIVVDALDSTGARLVLN"
BASE COUNT         1081 a         1219 c         1528 g         1138 t
ORIGIN      
        1 aggcagtcag attttcaccc ttctgcgagc gggtccgaag agtggaggaa cttgatgcgg
       61 gagcactatg cagggtatgg ccagtgcggt gcttttgtgg agcgcgctgt tgatctcgcc
      121 atccatagac tggcagccgc ttggggagaa ggacgtgatt accgggtttt cctggccttt
      181 gcaggcgatg attcggacgc gtcaggggtg tgtgagatct tccgccacaa aggagtggcg
      241 aagatagaga ggctgtacgt gcgaaagaac agtcgcatgc agggcatagg cagcagtatg
      301 atcatgcatg cagtggacta tgctcttcgg gcaggcgatg atctggcata tctcgttgcg
      361 gactcattgg attggccgag gcgtatgtat cgcaagcttg gttttgagga tctgatggag
      421 attgtggaat ggacgcgcat atggggcggg agcgcggatg atgagggtta tgttacatgg
      481 tgagcgcttc gcggcatgct ctgcacaggt gacgtagccc caagggaaag taacccgcat
      541 ccggtgagga cttggtccca aactcccata gccttaacta gccgtttcat gccgaatgca
      601 agtttggcgg gggtcgtgcc gaaacctgtc tgttttcgag ccacgatccc cgccctacgt
      661 actctggcta tcatatagcc tatatagagt gtagtcggtg ccaacgatac tgatgttcat
      721 gcgccaagtc tgttgaccgg ccgggtgcgg aatgctagta ttatggcaca tgtcgaaggg
      781 agctgaatgg tatgcgcgag aaggttgagg cccttttgga tgagataagg ccgtcgcttc
      841 aggcagacgg cggggacgtg gaactcgttg acgtgactga ggacggcatc gtaaaggtca
      901 ggctgaaagg cgcttgcgcc gggtgtccta tgtcgactct caccatcaaa aatggtattg
      961 agagggtgct gaagaacgag atacccgaag taaaagcggt tcagtctgta taggcttcgc
     1021 acaatcgcaa gctcagacct tgaaggacga ggcggcggtg gatgtgccgt cgccttttgc
     1081 gtggctaatc gaagatgaag agtcctagtg cttcataggt ccgcatgtct acgattcccg
     1141 tcactggaag ccccttgtct cgttgaagct gcattatggc gcgttcggtt ccggggccgt
     1201 ataccccgtc aaagccagca tgctcatagc cgagtttggc aaggcgtttc tgcacctcca
     1261 gcacgtaact tcctttggcg cccggctcga tccggggacg gaatgacccc aaagtgccga
     1321 agtggccggc ggttatgact accggcgtcc ctacgggaac cattgagtag agttcttcga
     1381 catcgcggtt gagcattctg atgcagccgc cagacttgcg ttgtcccaca gtccaaggct
     1441 tgtttgttcc gtgtatcccg tatttccccc acgggacgtt aagtcccatc caccttgtgc
     1501 cgaatccccc ggcccatgcc gccttctcta tgactttgaa ctgccctaaa ggcgtgggcg
     1561 tagatggctc gccgacgccg acccggtact gcttgatggg caaaccgtcc tcgaggagtg
     1621 tgatggtgag gttggatgta tcgataagga gagcgcgcca gcctggcggc agtccctttc
     1681 cggttgtgcc ctcttgttcg gtttggggtt tggccggcga agcctttgct ttggacttgg
     1741 cttcgggccc cgccccgatt tgctcgtccg ttgtttttgc gatgggaatg atgtccccac
     1801 tcggttccaa tgaagctgcg acaatctgtt cgcatactcg gccaagggcc atccatacgg
     1861 acaggtcaac tatgccgtct tcagacaatc caacactggc ctgaaaagcc cgcacggctc
     1921 cttccgttgc aggtccgaag agcccgtcga catgtcctgc aggaaatccc gcaaccgcca
     1981 gcccttcctg taaatcgcgg acttgctttc cgaccattct gggctcgcac agccacagct
     2041 tgtgtagatc cccacattcg cagagcgttg ccgaccgagg ctccgattcc gattctggag
     2101 atgcgcctcc gccatcgtcg attggcacta cggctccggc ggctgactca tgatcctgcg
     2161 atagctttct gctgccgggc caaaccgccg attcttctgc gtttgcaggc tcgctcctga
     2221 acccattggg tgtttccgga gtttgcgggg agtatccttt ggagaataca acagcgcaga
     2281 tgatgcccgt aaggacaagg gctgccaagc gaagagggcc tcggccgtct ctttcagggc
     2341 ttctcgtcaa cgcaaccacc cactagaagt cgatgtgttt ccagcatagt ctttccagca
     2401 acatcatggt taacgcattc cagtaagcag gtatgcggaa aaaaggctag aatacatatt
     2461 caaatagtca acaatggccg gccaacatat gccgacacag ttgaaggggg cttatggatg
     2521 cggggagaga tgcgggcaat atccaagatg aggcccgagc caggcgcaga gctgataacg
     2581 gcgcctattc ctcaaccggg aagaggagaa gtactggtcc gggtgcgtgc aacagccata
     2641 tgcggcactg acgtccacat atatgagtgg gatccgtggt cgcaggcccg agttacaact
     2701 cccagaatct ttgggcatga gttctgtgga gatgttgtgg aagttggaga gggcgtaacc
     2761 aaggtcaaag aaggtcagtt cgtatccgtt gagacgcaca ctgcatgcgg caagtgcttt
     2821 tactgtctga ctgggctcgc tcacatctgt cagaatgcga agatcatggg catagaccgc
     2881 gatggatgtt ttgcagagta tgttgttgtt ccggagttca attgctggat atgggatatc
     2941 gatatatcgg acgaggtagc ggcgattcag gacccatttg gcaacgcagt ccatacgaca
     3001 cttgccactg atcttgtggg gaaaacagtc cttataacag gaatgggccc aatcggcttg
     3061 tgcgctatca gtgtcgccaa agccgccgga gctactgagg tgtacgtaac cgatgtgtct
     3121 gattacagac ttgacctggc ttgcaagctt ggggcagatc ttgcgatcaa cccgaagcgg
     3181 gaggatcccg tagcggctgt gcgcgaggcc accggagggg taggcgccga cgtgctgctg
     3241 gagatgtcgg gaagccccag cgctattgtt caggggtttg atgcccttcg aaaggcggga
     3301 ttcgcatcac ttctgggaat cccttctgag ccgatcaagt tggacctggc caatgcgatc
     3361 atattcaaaa gcgccactgt tcagggcata aatgggcgca ggatgtttga gacttggttc
     3421 caggcaaggt cgctgcttaa ggcgggcctc gatatcacgc cggtgataac ccacaagatg
     3481 tcgctatggg atgatctgga tgaaggaatg gagctcatga tgtccggcca gtgcggcaag
     3541 gtaatcctct atccgtagcc gcggggggcc ggggttttta agaattgggc ggaaattaac
     3601 agtgtcttga ggcgttggtt gacatagctc cgccatgccc taggcaacac tgaccgccgc
     3661 cgcgggaggg atttcatatg ccggaagtca tctattccga agagaagcta aaggcccaag
     3721 acggtgtcga actcttttgc cgggtgtgga agggcgtgag tttggagggc gaacctgctc
     3781 tgaaggcgcg ttccacaatt gtcatagcac acgggcttgc ggaccattcg ggcaggtatg
     3841 agcatgttgc aaagttcttt gcccaaaaag gatatgttgt ttacgcctat gaccaccggg
     3901 ggcagggcaa gtcagaaggc gtgcggggac atgtcaacaa attctcgcaa ttctatgatg
     3961 atctttggtt tgtgatccgt agagccaagg gccgcaatcc cggaaagaaa tgcttcttgg
     4021 tggggcattc gatgggcggg ttgattgcct taggctatgc agccagacgt ccgaagacga
     4081 taaacggttt gatagtgact tcgccatgtt tggctttgcg aatgagggta tcgccgataa
     4141 agaaggggtt cggcacgttc atctcgtggc ttttcccgaa gatggctgtt gacacaggga
     4201 tagcggcaga ggggctttct catgactcga atgtagtgga agcctacaag aaggatccaa
     4261 ttcgctaccc caagatcaca accaagtttt atgtggagtt cgacaaggcc atgaagaatg
     4321 tgcagagctt ggcgaaatcg atcgtcactc cgtgcctttt catgcctggg ggagatgacc
     4381 cgatctgttc cacagaggct accatcaagt tctacgaaaa tgtgcctgag actaccgata
     4441 agaagttgat agtctgggac gggctttatc atgagatcat gaacgagccg gaaaaggaca
     4501 aagtgctcga tacgatggcc caatggattg cagagcggga gggtaagtag tgcccgcgct
     4561 gagcagtgct gagcgccagc ggtacagcag gcagatacgc cttgccagag tgggcgaagt
     4621 tggtcagggg agaatccgag ctgccagagt cttgattgtg ggcgttggcg gcctgggttc
     4681 tcctattgct ctgtacctgg ccgcagcggg ggtgggcgta atcggcctcg ttgaccccga
     4741 cgtggtggag ctatcgaatc tgcagaggca gatagcccac accacctgcg acatcgggag
     4801 gcttaaggtt gattctgctg agcgaaggat cctggctcta aactcggagg tgcaggttga
     4861 gaagtgggct gtggcgctcg atatcgataa cgcgggggat ttggtagggc ggtatgacat
     4921 agtggtggat gccttagatt ctaccggggc acgattggtg ctaaac
//
LOCUS       DAOSAI010000168         4945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10745_length_4945_cov_4.236401, whole genome shotgun sequence.
ACCESSION   DAOSAI010000168 DAOSAI010000000
VERSION     DAOSAI010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10745_length_4945_cov_4.236401"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLM83_06570"
     CDS             complement(<1..559)
                     /locus_tag="PLM83_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOK71102.1"
                     /translation="MEPLVEVKSLKKYFGVGGGTFKSKEGTLKAVDDISFSIPRGETL
                     GLVGESGCGKTTAGRTICRLYEPTAGEVIFDGIPIFSLDPKEMLPIRRKMQMIFQDPF
                     ASLNPRMTVGDIIGEPIDIHNLASSKAERLDMIHELLHTVGLSPEHATRFPHEFSGGQ
                     RQRIGVARALAVEPSFIICDEPISAL"
     gene            complement(559..1569)
                     /locus_tag="PLM83_06575"
     CDS             complement(559..1569)
                     /locus_tag="PLM83_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71103.1"
                     /translation="MDRLLEVRNVSTSFFTYSGEVKAVRDVSFHVDTGEAIAIVGESG
                     CGKSVTALSIMQLVAPPGKITDGEIFFNGRDLLKLSENQMQHVRGNEIGMIFQDPMTS
                     LNPVLTIQTQIVESIMLHQNVTREQAVKRCVELLHMVGIPSPERRLGSYPHEFSGGMR
                     QRVMIAMALACNPMLLIADEPTTALDVTIQAQIISLMAELKKKLNMSIILITHDLGVV
                     AGLAQRVLVMYAGKVIETGTVRDIYYHAQHPYTWGLLKSVPRLDAEEKKKLVPILGQP
                     PDLISPPTGCPFAPRCDYAMSICANEPPDYTKLSDHHKVACWLQYPLAPTYDRVERGG
                     VA"
     gene            complement(1600..2514)
                     /locus_tag="PLM83_06580"
     CDS             complement(1600..2514)
                     /locus_tag="PLM83_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71104.1"
                     /translation="MEYSAEMFKPINKDIDSAEAIVRPSVTYWQDAWRRLKKNRMAMV
                     GLWTIVAILVIAIFGPMVSPYSYSDQNLEEQNQYCSSKHWLGTDDLGRDLLTRIMYGA
                     RISLTVGFLASLLNLTVGVIYGGISGFYGGKIDNIMMRIVEVLNAIPMLLYVILLMVI
                     LEPGLQNILIALGLVYWLGMARIVRGQVLSLKEQDYVLAARSIGASNWQIITKHLIPN
                     AMGPIIVTATLNIPSAIFTEAFLSFIGLGVNAPMASWGVLASDALQSFRSYPWQLFFP
                     AIAISITMFAFNFLGDGLRDALDPKQRK"
     gene            complement(2521..3450)
                     /locus_tag="PLM83_06585"
     CDS             complement(2521..3450)
                     /locus_tag="PLM83_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71105.1"
                     /translation="MGSYVLRRFISVILVIFVVATATFFLMHAIPGGPFKREKALPPA
                     IQRNIEERYKLNDPLWKQYVDYIGNLLRGDLGPSYKYLGRSVNDIIREGFPISAHLGL
                     VSVVFALLVGVPCGIISALKQNQWQDNFIMAFAIIGVSVPSFVMATLLMYVFAVKLQW
                     LPAAMWGTPKHVILPMLALAGFPTAFFARLMRSSTLDVLSQDYIRTARSKGLSWGAVV
                     YKHVIKNAILPVVTYLGPLIAGIITGSFVVENIFAIPGLGRYYVTSIYNRDYTTIMGV
                     TIFYSALLVMLNFLVDIAYCWIDPRIKLTDAKE"
     gene            complement(3561..>4945)
                     /locus_tag="PLM83_06590"
     CDS             complement(3561..>4945)
                     /locus_tag="PLM83_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOK71106.1"
                     /translation="QPAIAESWTISKDGLVYTFKLRKTKWSNGQPVTAHDFEWAWKRA
                     LAPETASEYAYQLWYIKNGRPYNEGKLKDPSKVGVRAVNDYTLQVVLENPTPYFLQLC
                     NFPTLMPVYRKVVEADPEGWFQNPKNYIGNGPYKMVSWQHNSKIELVPNPHYWNRKKV
                     GMERLDLYLVDDIQTALSMFETGQLDWFDAPPAAEIERLEKQGLLHKSEYIGTYYYMF
                     NVTKKPLNDVRVRKALTMAIDRQTLIDRVVRGGQLPALAYVPYGLGDATPDKDFRKVG
                     GDYFKEDLAAAKKLLAEAGYPDGKGFPTLTILYNTSENHKKIAEAIQEMWKKNLGIDV
                     RLTNQEWQVYLDSRTQLNYEVARAGWIGDYLDPMTFLDMWVTGGGNNDTGWGSKKYDE
                     LIYAAQAELNTAKRAKMLHDAETILMTEMPIMPIYFYVNLTVIKPWIKGAFRSPLGFI
                     DFSTAYVLEH"
BASE COUNT         1153 a         1409 c         1222 g         1161 t
ORIGIN      
        1 cgagcgcgga tatgggctcg tcgcatatga taaaactcgg ctccacagcg agagcccgcg
       61 caaccccgat cctctgcctc tggcccccgc tgaactcgtg cggaaaccta gtagcatgct
      121 cagggctgag gcctacagtg tggaggagtt cgtgtatcat gtcgagcctc tccgccttgc
      181 tgctcgcaag gttgtgtatg tcaatgggct ccccgatgat gtcacccaca gtcatgcgcg
      241 gattcaaaga agcgaaagga tcctggaaga tcatctgcat ctttcgcctt ataggcagca
      301 tttccttggg atcgagcgag aagatgggaa tcccgtcgaa tatgacttcg ccggcagtag
      361 gctcgtaaag cctgcatata gtcctgcccg cagtcgtctt accgcaaccg ctctcgccca
      421 caagccccaa agtctcgcct cggggtatgc taaagctgat atcatctaca gctttcagcg
      481 tcccttcctt gctcttgaag gttcctccgc caacaccgaa gtacttcttc aggcttttca
      541 cctcgaccaa cggctccatc aggccacgcc ccctctctcc acgcgatcat atgtcggcgc
      601 caacgggtac tgcagccaac atgccacctt atgatgatcc gagagcttag tatagtcagg
      661 cggctcattg gcgcagatcg acatcgcgta atcgcacctc ggagcgaacg gacacccggt
      721 cggaggactt atcaagtccg gaggctgtcc cagaatgggc acaagttttt tcttctcttc
      781 cgcgtccagc ctcggtacgg acttcagaag gccccatgtg taaggatgct gggcatggta
      841 gtagatgtcg cgtactgtac cggtctctat cacttttcca gcgtacatca ccaacacgcg
      901 ctgggccaaa cccgcaacaa caccgagatc gtgggtgatc agtatgattg acatattgag
      961 cttcttcttc agctccgcca tgaggcttat aatctgagcc tgaatcgtga catctagagc
     1021 cgttgtgggt tcatctgcaa taagaagcat cgggttgcag gccagagcca tcgctatcat
     1081 caccctctgc ctcatgcctc cgctgaactc gtggggatac gatcccaaac ggcgttcggg
     1141 cgaaggaatc cctaccatgt gaaggagttc cacgcatctc ttgaccgcct gctcccgggt
     1201 gacattctgg tggagcatga tgctttccac tatctgagtc tgaatcgtca aaacagggtt
     1261 tagagacgtc ataggatcct ggaatatcat cccgatctcg tttccgcgca catgttgcat
     1321 ctgattctcg ctgagcttca acaaatcgcg tccattgaag aagatctccc cgtctgtgat
     1381 cttcccaggc ggcgccacca gttgcattat ggaaagcgcc gtgacgctct taccacaccc
     1441 agactcgcca acaatagcta ttgcttcacc ggtgtccacg tggaagctca catcacggac
     1501 tgccttgact tcgcctgaat atgtgaagaa cgatgtgcta acgtttctga cttccaaaag
     1561 tctgtccaag aggtatcccc cctactctat gtccaccggt tacttgcgct gcttgggatc
     1621 gagcgcatct cgaagaccgt ctccgaggaa gttgaacgcg aacatagtaa tgcttatagc
     1681 tatagctggg aagaagagct gccagggata tgacctgaag ctctgtagcg cgtcggatgc
     1741 caacacgccc cagctggcca ttggcgcatt cacgccaagt ccaatgaagc tcaagaaggc
     1801 ttcggtgaat atagccgacg gtatattgag cgtggcggtg actatgatcg ggcccatcgc
     1861 atttggaatc aagtgtttgg tgattatctg ccaattgctt gccccgatcg accgcgctgc
     1921 gagaacgtag tcctgctcct tcagactaag aacctgacct ctcacgatcc tcgccatacc
     1981 gagccagtat acaaggccca gcgctatcag gatgttctgc agaccaggct cgagaatgac
     2041 catgagcaat atgacgtaaa gcagcatagg aatggcattg agcacctcga ctatgcgcat
     2101 catgatattg tcgatcttcc cgccgtaaaa acccgaaatc ccgccataga tcactcctac
     2161 agtgaggttg agcagactcg ccaaaaaccc gaccgtaagc gatatcctgg ctccgtacat
     2221 gatgcgcgtc aacaggtcgc gccccagatc atcagtgccc agccaatgct ttgaactgca
     2281 atactggttc tgctcctcta gattctggtc agagtagctg tacggcgata ccatggggcc
     2341 gaatatggca atcacgagaa tcgccacgat ggtccatagc cccaccatgg ccatacggtt
     2401 cttcttcaac ctgcgccacg catcttgcca gtaggtaact gacggacgca caatcgcttc
     2461 cgcgctatcg atatccttgt taatcggttt gaacatttca gcgctatatt ccacagctgc
     2521 tcactccttc gcgtccgtca gcttgatcct gggatcgatc cagcagtagg caatgtctac
     2581 caagaagttc agcatgacca aaagcgcgct gtagaatatc gtaacaccca ttatggtagt
     2641 gtaatccctg ttgtagatgc tcgtcacata atagcgtcca agcccgggta tggcaaagat
     2701 attctctaca acaaagcttc cagtgattat tcccgcaatc agaggtccca aataggtaac
     2761 caccggaagt atagcgttct tgatcacgtg cttgtagacg acagcacccc aggaaagtcc
     2821 cttggatctg gctgttctga tgtagtcttg cgatagcaca tccagcgtac tagatcgcat
     2881 caaccttgca aagaaagcag tggggaaccc cgccagcgcc agcatgggaa ggataacgtg
     2941 tttcggagta ccccacatag cagcgggcag ccactgaagc tttacagcaa acacatacat
     3001 gagcaatgtt gccatgacga agcttggaac agacactcct atgatggcaa acgccattat
     3061 gaagttatcc tgccactgat tctgtttgag ggcggatatt atcccgcacg ggacccccac
     3121 caaaagggcg aaaacgacgc ttacaagtcc cagatgggcg ctgataggga acccctcccg
     3181 aatgatgtca ttaacagatc ggcccaaata cttgtaggaa ggccccagat cgccccggag
     3241 caagttgccg atgtagtcga catactgctt ccatagcggg tcattcagtt tataccgttc
     3301 ctcaatgttc ctttgaatcg caggaggcaa ggccttctct ctcttgaacg ggccgcccgg
     3361 gatagcgtgc atcaagaaga acgtcgcagt cgccaccacg aatataacca gtattacaga
     3421 aatgaacctt cgtaggacat atgagcccac caatatcagc tcccgtcaaa tgccagagcc
     3481 gggggcatgc tccggtaacc cgtgcatgcc cccgggcact gatggttcat atagatacgt
     3541 gcgcgcctat aagcgcctta tcaatgctca agcacgtatg ctgtcgagaa gtcgatgaag
     3601 cccagaggcg atctgaaagc gcccttgatc cacggcttga tcacggtcag gttgacatag
     3661 aagtagatcg gcattatcgg catctcagtc atgagaatgg tctctgcgtc atggagcatc
     3721 ttcgcacgct tagctgtatt cagctctgcc tgagctgcat agataagctc atcgtacttc
     3781 ttgctgcccc atcccgtatc gttgttcccg ccgccggtca cccacatgtc gaggaaggtc
     3841 atgggatcaa gatagtcacc aatccagcct gcacgggcca cctcgtagtt gagctgggtg
     3901 cgggaatcga ggtagacctg ccattcctgg ttggtcagcc taacgtcgat cccgaggttc
     3961 ttcttccaca tctcctggat cgcctcggcg atcttcttat ggttctcgct cgtgttatat
     4021 agaatcgtca acgtcgggaa gcctttgcca tccggatatc ctgcttcagc aagaagcttc
     4081 tttgcagcag cgagatcttc cttgaagtaa tccccgccaa ccttccggaa gtccttatca
     4141 ggcgttgcat cgccgagccc gtacggcaca taggccagtg ccggaagctg tccgcccctg
     4201 acgacacggt ctatcagagt ctgacggtcg atagccatgg tgagagcctt gcggactctc
     4261 acgtcgttga gaggcttctt agtgacgttg aacatgtagt agtaggttcc gatgtactcc
     4321 gacttatgta gtaacccttg cttctcgagg cgttctattt ctgcagcagg aggcgcatcg
     4381 aaccaatcca gctgaccggt ctcaaacatc gaaagagcgg tctgaatatc atcgaccaga
     4441 tagaggtcga gcctttccat gccaaccttc ttcctattcc aatagtgcgg gttgggcacc
     4501 agctcgatct tggagttgtg ctgccacgat accatcttgt aagggccgtt tccaatgtag
     4561 ttcttgggat tctggaacca cccttcagga tccgcctcca caaccttgcg gtaaaccggc
     4621 atcaaagttg ggaagttgca gagctgaagg aagtaagggg tcggattctc caacacaacc
     4681 tggagagtat agtcattgac tgcccttacg ccaaccttag acgggtcttt cagctttccc
     4741 tcgttatacg gtcttccgtt cttgatgtac cacagctgat atgcatactc ggaagctgtc
     4801 tcgggcgcca gtgcgcgctt ccacgcccat tcaaagtcgt gggcggttac aggctgccca
     4861 tttgaccact ttgtcttgcg cagcttgaag gtgtagacaa ggccatcctt gctgatcgtc
     4921 cagctctccg cgatagctgg ctgag
//
LOCUS       DAOSAI010000169         4939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10766_length_4939_cov_2.925471, whole genome shotgun sequence.
ACCESSION   DAOSAI010000169 DAOSAI010000000
VERSION     DAOSAI010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10766_length_4939_cov_2.925471"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLM83_06595"
     CDS             <1..452
                     /locus_tag="PLM83_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71107.1"
                     /translation="SAVGDRLSDLSPADRYRVDLETRSAAILSKVEGAGTVSVQITLE
                     GGTYREYAVNSVEEYKNVDEKMSSGEARVTTEKRETRDVVLPSDPSSGRGQSPILVLE
                     RSPAISGVLVAASGASDSRTKRELAEAAQTLLGVPAHRVMVVVAKSP"
     gene            549..1259
                     /locus_tag="PLM83_06600"
     CDS             549..1259
                     /locus_tag="PLM83_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HOK71108.1"
                     /translation="MIKKTKQYFGQRGDRFWLKASYLMLAVAVVILGITAIVDPDLYR
                     RRSSAPAVDAEGDNGLSVTEDDGSEEDLSEGEESADLCGATLPTSVLSDGNDQYGSVG
                     GPGSVTPFGAEPFIMPSGADFFEELKIDRDKTRSREREVIQDLIDDPNTHPDVRKRAQ
                     EALLSLAERARREAEAESMIQAKGYEQAVVFLSEAGASVVVKSGRLEEAEVKRIGDAV
                     SAATGVELRRISIMEREP"
     gene            1382..1765
                     /locus_tag="PLM83_06605"
     CDS             1382..1765
                     /locus_tag="PLM83_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOK71109.1"
                     /translation="MELMEDPGMGSVKIANEVVAVIAGLATIEVEGIAGMSGGIAEGW
                     SELWGKKNLARGVKVEVGEQQAAVDLNVVVKYGAVIPEVCEEVQRNVKRTIEIMTGLE
                     VVQVNVHVTNVFIEEKETSTDVRVR"
     gene            1804..2343
                     /gene="amaP"
                     /locus_tag="PLM83_06610"
     CDS             1804..2343
                     /gene="amaP"
                     /locus_tag="PLM83_06610"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HOK71110.1"
                     /translation="MKTIDRVILIATAVIIVFLGSVVLASVSGGGFPNAFAIAFYDSV
                     ASNRIAAAIIGIAIVLVTLYVIMMALRVEESAEPIITGTGLGDVQISMAAVEAIAQRA
                     AQKVDGVRNVEAAAEGNEDGITLHARINVYPDVSIPEVCENLERELSEAMHAVIGLPV
                     KSIHTVVKGVSRMESKPRP"
     gene            2395..2625
                     /locus_tag="PLM83_06615"
     CDS             2395..2625
                     /locus_tag="PLM83_06615"
                     /inference="COORDINATES: protein motif:HMM:NF021542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HOK71111.1"
                     /translation="MPWFGDGRFNVPEGSSGQFVGAIVGAIFGLLVLIAGFWRALAFA
                     LFVGIGFIVGRYIDVNDEFRERFARRFGRRRN"
     gene            2642..3199
                     /gene="nusB"
                     /locus_tag="PLM83_06620"
     CDS             2642..3199
                     /gene="nusB"
                     /locus_tag="PLM83_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOK71112.1"
                     /translation="MRSERRRAREAAMRALYQMDMSGCTSEDAISYAVSSSSDLDLPE
                     RAQGYASQLVCAAVERMTEIDAVMAGIAHDWAVQRMSPTDRNIMRIAVCEMLYLKDVP
                     VATAIDEAVEIAKLYGTADSPRFVNGILGALAERIKDGQGPADGADAGEGRAESSDAA
                     SGGDVEGDSGGKCLGGAHEGRDTRV"
     gene            3174..4157
                     /locus_tag="PLM83_06625"
     CDS             3174..4157
                     /locus_tag="PLM83_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HOK71113.1"
                     /translation="MKVGTLGFDTSCYTTSVALVISGILAYDGRAPLQVSKGKVGLRQ
                     SEALFQHVRNLPRLVEDACDVARRESCDIVAVGASAWPRRMEGSYMPVFLASSGYGRA
                     VAAALEAPCLEISHQEGHIAAARWSSGLESRGGFLALHLSGGTTELLSVHDRFEMELL
                     GGTGDLNAGQFIDRVGVALGLPFPAGPDLERLAGHGVQGRVRLPVAVSGLKVSFSGPC
                     SAAMRELDSGAGHADMAYAVLECIGRSVGAMCCEGLRFFRAQELLVAGGVASNYIIRR
                     ILSETIEDQLPGAKVYYASPEHSRDNAVGCALLAWQMHLEGMRESREEGKE"
     gene            4164..>4939
                     /gene="xseA"
                     /locus_tag="PLM83_06630"
     CDS             4164..>4939
                     /gene="xseA"
                     /locus_tag="PLM83_06630"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOK71114.1"
                     /translation="MAGRYKAGTSGASDGGFGPHGSIGTCGACGSDGIFSASGIPNLP
                     GMPQMAGKVGSYEIFTVSEITRRVARALDRDPVLGDIWVNGEIANFTHHSSGHIYFTL
                     KDDSSRLRAVMFRGSNIGLRFRPADGMRVYAHGYVGVYERGGEYQLYVDLMEPAGLGE
                     LYLAFQQLRAKLEAEGLFDSAAKRPLPKFPRRLGVITSPTGAAVRDIITVTRRRWPGI
                     DILVIPALVQGSDAPASIVQAFDRAGQVRDIDVIIVGRGGA"
BASE COUNT         1038 a         1167 c         1626 g         1108 t
ORIGIN      
        1 cgtcggccgt gggcgaccgg ctttccgact tgtcccctgc agacagatac cgggttgacc
       61 ttgaaacgag atcggcagct attctctcaa aggtggaggg agcaggcaca gtcagtgtcc
      121 agataacttt ggaaggcggc acataccggg agtacgcggt caattccgtg gaggaataca
      181 aaaatgtgga tgagaagatg tcgtcgggcg aggcgagggt gacgactgag aagcgagaaa
      241 cccgcgacgt cgtcttgcct tctgatccct cttcagggcg cggacagtct cccatcttgg
      301 ttttggaacg ctctcctgct atctccggag tgctggtggc ggcgtcgggc gcatcggatt
      361 caagaaccaa acgggagctt gccgaagctg cacagacgct ccttggtgtg ccggctcaca
      421 gggtcatggt cgtggttgct aagtccccgt aggcagctag cacggatgtg atcgaaaact
      481 tgcactcggt tcttgtcggc ggaaatcttc gaattgcaga cgcgcagacc gggaggtggg
      541 caatgtccat gataaagaag acaaagcaat actttgggca gcgtggtgat agattctggc
      601 tcaaggcatc ctacctgatg ctggcggtgg cggtggtgat cctgggaatc acggcgatcg
      661 tagaccccga cctctatcgt cgccgctcat ctgcgccggc agttgacgcc gaaggcgaca
      721 acggtttgag tgttacggaa gacgacggta gtgaggagga tctgtcggaa ggagaggagt
      781 ctgccgattt gtgtggagcg actctgccga catctgtcct ctcagatggt aatgaccagt
      841 atgggtccgt cgggggcccg ggctcggtca cgcccttcgg cgccgagcct ttcatcatgc
      901 catcgggagc agactttttc gaagaactca agatcgatcg cgacaagacg cgttctcgcg
      961 aacgcgaagt gatccaagat ctcatcgacg atccgaatac tcacccagat gtgcgcaagc
     1021 gcgcacagga ggctcttctt tccctggcgg aacgagcacg gcgcgaagct gaagctgaaa
     1081 gcatgataca ggcaaaagga tacgagcagg ctgtggtctt tctatcggag gctggcgcaa
     1141 gcgtggtcgt aaagtcggga agacttgaag aagcggaagt caagcgtata ggcgatgcgg
     1201 tgtcggctgc tacaggcgtg gagttgcggc ggatcagcat aatggagaga gaaccgtgaa
     1261 ggataatcta ggcatgcggt gaattcattt ctatgattgg ctacttgggg accggcagtt
     1321 ctgttataat gctaatatga tcagcgaggg ttcctctgga cgcttgccag gaggtgtgaa
     1381 tatggagctt atggaagatc cgggaatggg atctgtcaaa atcgcaaatg aagtggtagc
     1441 agtcatagcc ggccttgcaa ctatcgaagt tgaaggcatt gcagggatga gcggtggaat
     1501 agcggaaggc tggagcgagc tgtggggcaa gaagaacctg gcccgtgggg tcaaggttga
     1561 ggtcggcgag cagcaggcgg cagtcgatct gaacgtcgtg gtgaaatatg gcgcggtaat
     1621 acctgaggtc tgcgaagagg ttcagcgcaa cgtcaaaagg acgatcgaga ttatgaccgg
     1681 cttggaagtg gttcaagtca atgtccatgt gactaacgtg ttcattgagg agaaggagac
     1741 atctaccgat gttcgggttc gttagcaata tcgcctgtta ccttgatgac gggggtggcc
     1801 acattgaaaa ccattgaccg agtgattctc attgccaccg ctgtaatcat agtcttcctc
     1861 ggttcggtgg ttcttgcgtc tgtttcgggg ggaggattcc ccaatgcctt tgccattgct
     1921 ttttatgatt cagtggcgag caataggatt gcagctgcca taatcggcat cgcgatagtt
     1981 cttgtaactc tgtatgtgat catgatggct ctcagagtag aggagtcagc ggaaccgatt
     2041 atcactggga caggtcttgg cgatgtgcag atttccatgg ctgctgtgga ggccattgcg
     2101 cagagggctg cccagaaggt ggatggtgtc cgcaacgttg aggctgcagc ggagggcaat
     2161 gaggatggca tcacacttca tgcgcgcata aatgtttatc ctgatgttag catcccagag
     2221 gtttgtgaaa acctggaaag agagctttcc gaagcaatgc atgccgttat agggttgcca
     2281 gtcaaatcca tacacacggt tgttaaagga gtatcacgca tggagagcaa gccccgaccg
     2341 tagggcgctg agcgacgcat aaagtggggg caatgcctgg aggagatggt ccgcatgcct
     2401 tggtttggag acggcagatt caatgttccc gaaggtagct ccggacagtt tgtgggggct
     2461 atcgtaggcg ccatcttcgg gctgctcgtc ttgatcgcag gattttggcg agcgctcgct
     2521 tttgcgcttt ttgtgggaat cggttttatt gtaggcagat acatcgatgt taatgacgaa
     2581 ttcagggaga ggtttgcgcg gcggtttgga cgtcggcgaa actgacagga gtggttgacg
     2641 catgcgctca gaaaggcggc gggcccgtga ggccgccatg cgagcccttt atcaaatgga
     2701 catgtcaggg tgcacatcgg aagatgcgat ttcctatgca gtatcgagct cttccgacct
     2761 tgatcttccc gagcgggcgc agggatatgc ttctcagctt gtgtgtgccg cggtggagag
     2821 aatgaccgag atagatgctg ttatggcggg gatcgcccat gattgggcgg tccagcggat
     2881 gtcgccaacc gatcggaata tcatgaggat agcagtatgc gaaatgcttt atcttaagga
     2941 cgtgcctgtg gctactgcca tcgacgaggc cgtcgaaatc gccaagcttt atgggactgc
     3001 cgattctccg agattcgtca acggaatctt gggggccttg gctgagcgca tcaaggatgg
     3061 ccagggccca gcggatggcg cggatgcggg cgagggccgt gccgagtcat ctgacgcagc
     3121 cagcggcggc gacgtggaag gagattctgg ggggaaatgt cttggcgggg cgcatgaagg
     3181 tcgggacact cgggtttgat acgagctgct atacaacttc agtggctctt gtaatcagcg
     3241 gcatcctcgc ctatgatggc agggcgccgc ttcaggtttc caagggcaaa gtagggctca
     3301 gacagtcgga ggccctgttt cagcatgttc gtaacctgcc tcgcctcgtt gaggacgcat
     3361 gtgatgtggc gaggcgggag tcatgcgata ttgttgccgt cggggccagc gcatggccgc
     3421 gacgtatgga aggatcctac atgcccgtct ttcttgcgtc atcagggtat ggccgcgctg
     3481 tagcggctgc ccttgaagcg ccgtgcttgg agatatctca ccaggaaggc cacattgccg
     3541 cggccaggtg gtcttcggga ctcgaatctc gaggaggctt tctcgccctt cacctttccg
     3601 gaggcacgac cgagcttctc agtgtccatg acaggtttga aatggagctt ttggggggca
     3661 ctggcgatct caacgcgggg cagtttatcg acagggtagg ggtggctctc gggcttccgt
     3721 tccctgcggg gcccgacctt gagcgccttg ccggacacgg agttcagggg cgggtcaggc
     3781 ttccggtggc cgttagtggg ctcaaggtga gcttttctgg cccttgctcg gccgccatga
     3841 gagaattgga ttcaggggcc ggccatgccg acatggcata cgcggtgctc gagtgcatag
     3901 gcagatcggt cggcgcaatg tgttgcgagg gattgcgctt cttccgggcg caggagcttc
     3961 ttgttgcggg aggcgtggcg tcaaactaca tcatcaggcg tatcctctcc gagacaatcg
     4021 aagaccagct cccgggcgca aaagtgtatt atgcttcgcc tgagcattca agggacaatg
     4081 ccgtaggatg cgcgcttctt gcgtggcaga tgcatttgga gggcatgcgc gaatcacggg
     4141 aagaaggcaa ggagtgaatg tagatggcag gtcggtacaa ggcgggtacg tctggggctt
     4201 cagatggagg ctttgggcca catgggtcca ttggaacatg tggagcctgt ggatcagacg
     4261 ggattttcag tgcgtcgggg attccgaact tgcctggcat gccgcagatg gcgggaaagg
     4321 tagggtctta cgagatcttc accgtatccg agattacgcg tcgggtggcg cgggccctgg
     4381 accgagatcc ggttttgggt gatatctggg tgaatggaga aatagccaat tttacccatc
     4441 attcgtctgg acacatctat tttaccttga aggacgatag ttcgcggctt cgcgcggtga
     4501 tgttccgagg gagcaacatt gggttgcggt tccgccctgc cgatggaatg cgcgtttacg
     4561 cccatgggta tgtaggcgtt tacgagcgtg gaggagaata ccagctatat gttgacttga
     4621 tggagcccgc gggcctcggc gagctctacc tggccttcca gcaactccga gccaaactgg
     4681 aggcggaagg gctatttgac tctgccgcca agaggccgct gccgaagttt ccgagacgcc
     4741 ttggtgtgat cacgtcccct actggagctg cggtgcgcga cataataact gtgactcgaa
     4801 ggcgttggcc gggaattgac atactggtaa ttccagctct tgtgcagggc tcggatgctc
     4861 ccgccagcat agttcaggcc ttcgatcgag ccggtcaagt ccgtgacatc gacgtaatca
     4921 tagtggggag gggcggggc
//
LOCUS       DAOSAI010000170         4931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10798_length_4931_cov_3.449959, whole genome shotgun sequence.
ACCESSION   DAOSAI010000170 DAOSAI010000000
VERSION     DAOSAI010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10798_length_4931_cov_3.449959"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2471
                     /locus_tag="PLM83_06635"
     CDS             <1..2471
                     /locus_tag="PLM83_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOK71115.1"
                     /translation="AGYGAREAARDAEEDARLTEERKQQEINNWRLFYIGAAFSIPLV
                     VGMIADIFGIEALMFLIDPFVGFVLATPVVFVPGSRFYISAARTVAHGGANMDVLVAL
                     GTGAAYILSVVHTFIVSGPVYYEAAAVVITLVLLGKNLEHVAKGKTSEAIRKLAALSP
                     KTASVLQADGTEIQVPVDDVEVGDILIVRPGERVPVDGVIIEGFSAIDESMLTGESIP
                     VDRQAGDAITGGTVNGHGMLKMRATRVGSETALAQIIRMVEEAQGSKAPIQRLADVVS
                     YYFVPAVLVVAVVTFAGWMAAGIGASQALVNATAVLVIACPCALGLATPTAIMVATGR
                     GAELGILIRGGEHLERLSSVKAVVLDKTGTITAGVPSLEEVEVFAPHGREEALRLAAS
                     AERASEHPLGRAIVEAAEQAGIDIPMPDEFRTVPGRGIVATVEGRQVAVGTLRFLDEL
                     GVSVDREGLAAERRFGSGLVAGTAEETNGAGSEVPVTSLIAAGAVGASVARMQDEGRT
                     AVVQAVDGAVAAVYGIADQVRPGSVEAIKGIQDLGIDVYMITGDNARTAAAIAERVGI
                     PKPNVLAEVLPEDKAREVERLQEAGLAVAMVGDGINDAPALATADVGVAMSTGTDVAM
                     EAADITIVGNLGKLLDAIKLAKKTITIIKENLFWAFVYNTIGIPLAAFGVLPPVYAGA
                     AMSLSSVSVVSNSLRLRGFGKERPRGISRTEVKGVDNMPYGRKSTSDSKENMVAGPGS
                     TEKSAAAAGMVGDYSEALLNVAGMSCGHCKAAVEGALMGVVGVESAEVDLEAKSARVV
                     YDRNAAKPADLVRAVEDAGYEVLR"
     gene            complement(2608..4725)
                     /locus_tag="PLM83_06640"
     CDS             complement(2608..4725)
                     /locus_tag="PLM83_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71116.1"
                     /translation="MKHQLRTHLATALATLCLLAVSAGALGSSVTLLDGTTYDADLVG
                     FFTFDGVDHFVFRLTDGYLMACPRSDVMLIQVSKSSMGSTQVGQQVPPPAIAIPSTPI
                     SGPLFELSGIWSADLNGFGGEIRIEQSGTTLSGSVFGDTLANGRVSPDGSVAFERIVG
                     SGNQVYRGTVSRDASGNLIIEGTFDCNITGGKGLPWHAVLTKPSAGSPSAQLPTTQPS
                     QPAVPPALEPSYLQAEREPNYNIASANPIAVNTNVTGAISTDGDIDMYKVTVTTHGTF
                     HVTCTQPADFFMAILNEAGSQIAGDDLGSSGTTMVLNADVFEPGNYYILVRGYSGNIK
                     SDTPYTLRAEFREARDAFEPNDNVNLASAVVSGRQFDSFIYRPGDIDYYSVSVSGPAT
                     IKVTSTQPADYYLSIVDSSGTKELYGGDLGSKGTNMLLEGDVYDPGTYYVVMRGYNKD
                     IYEMHTPYTMTITVTHGGTDRFEPNDTPGQAAVPPLGQTFAAFISRPGDLDYYKVDIA
                     GPSQVVAESDQPVDLHLSLIDRDGKTVLLGDDYGHPGNKMVLTHDIFQPGTYYVLMRG
                     YNTNIYSMHTPYNMKITVKPALYDPAEPNNSANEAKSIQLGQPVTGVIATGTDEDWWQ
                     FTAPLLGKVRITCTQTADFRLRVVDATGKEITNQDLGNKGTLMDLTLQLTPLTRYYVV
                     VQPYSSSCYNMFEAYQLIVGSAI"
BASE COUNT         1008 a         1202 c         1554 g         1167 t
ORIGIN      
        1 ctgcgggata tggtgcgagg gaggcagcac gcgatgctga agaggatgct aggctcacag
       61 aggaaagaaa gcaacaggag atcaacaact ggcgactgtt ctacataggc gctgcattca
      121 gcattccgtt ggttgtgggg atgatagccg acattttcgg gatagaggcg ttgatgtttc
      181 tcatcgaccc cttcgtgggc ttcgtcttgg ccacgccagt ggtgttcgtg cctggatccc
      241 gcttctatat cagcgccgcg aggacagttg cccatggcgg agccaacatg gacgttcttg
      301 tagccttagg cacgggcgct gcttacatcc tcagcgtggt ccacaccttc atcgtttcag
      361 gccccgtcta ttacgaggcc gctgcagtag tcatcacctt ggtgctgctg ggaaagaacc
      421 tggagcatgt ggcgaagggc aagacgagcg aggccatacg gaaactcgcc gctttgtctc
      481 ctaagaccgc ctcggtgctg caggctgacg ggaccgaaat tcaggttcct gtggatgacg
      541 tggaagtcgg cgacatcctg atagtcaggc cgggtgaacg cgtgcccgta gacggcgtga
      601 taatcgaagg cttttctgct atagacgagt cgatgctcac aggcgagagc atccccgtgg
      661 acaggcaggc gggcgacgcc ataacaggag gcaccgtcaa cggccacggg atgctcaaga
      721 tgcgggcgac ccgggtgggc tcggagactg cgcttgctca gattatccgc atggtggaag
      781 aagcgcaggg aagtaaggcg cccatacagc gtttggcgga tgtggtgtca tactatttcg
      841 tccccgcggt tctggttgtg gctgtggtta cgtttgcagg atggatggct gcaggaatag
      901 gcgcgtctca ggcattggtg aacgccacgg ctgtgctggt catcgcgtgt ccttgtgcgc
      961 tcgggctcgc gaccccgacg gctatcatgg tagccactgg gcgtggcgcc gagctgggta
     1021 tcttgatacg tgggggagag catctggaaa ggcttagctc agtgaaggca gtagtcctcg
     1081 acaagactgg gacgattaca gcgggcgtgc cgtcgcttga agaagtcgaa gtctttgcac
     1141 ctcacgggcg tgaagaggcg cttcgactcg ccgcttctgc tgaacgggct tcagagcatc
     1201 cgctcggcag ggcgatagta gaagcggctg agcaggcagg gatagatatt ccgatgcccg
     1261 acgagtttag gacggttccg gggcgcggca tcgtcgcaac tgtggaagga agacaggttg
     1321 ccgtcggaac gcttcgcttt ttagatgaac ttggcgtatc cgtggaccgt gaagggcttg
     1381 cagccgagcg caggttcggg agcgggctgg tggcggggac ggctgaggag acgaatgggg
     1441 ctggttcaga agtcccggtt acgagtttga ttgccgcagg cgctgtgggg gcctctgttg
     1501 ccagaatgca agatgagggg cggaccgccg tagttcaggc ggtggacgga gcggtcgctg
     1561 cggtctatgg aatcgccgat caagtacggc cgggttcggt ggaggccatc aaggggattc
     1621 aggacctcgg catagacgta tatatgatta ccggtgacaa cgcgcgcaca gcggcagcta
     1681 tcgccgaacg tgttggaata cccaaaccca acgtcctggc tgaggttctt cctgaggaca
     1741 aggcgcgcga ggttgaacgg ctgcaggagg ctgggcttgc cgttgccatg gtgggtgatg
     1801 ggataaatga tgcccctgcc cttgccactg cggatgtagg ggttgccatg agtacaggga
     1861 ctgatgttgc catggaagct gcggatatta ccattgtggg taatttgggc aagctcctcg
     1921 atgccattaa gctggccaag aagactatta ccataattaa ggagaatctt ttctgggcct
     1981 ttgtctacaa taccattggg atccctcttg cggctttcgg agttcttccg ccagtgtatg
     2041 ccggggctgc gatgtcgttg agctcggtat cggtagtttc gaattcattg cgattgagag
     2101 ggttcggaaa agagaggcct cgagggatct ccaggacaga ggtgaaaggg gtagataata
     2161 tgccctatgg caggaagagc acgtcagata gcaaggagaa catggtggcc ggaccgggat
     2221 ccacggagaa atccgctgcc gctgccggaa tggtaggcga ttacagtgag gctctgctca
     2281 acgtggctgg aatgagttgc gggcattgca aagccgctgt ggagggtgct cttatgggtg
     2341 tagtaggagt cgagtcggca gaggttgatc ttgaggctaa gtccgccagg gtcgtatacg
     2401 acaggaacgc ggcaaagcct gcagacttgg ttcgtgcggt ggaggatgcc gggtacgagg
     2461 tgctgaggta gctctgtccc ggacagcttg gatgcacgat ggggtatggc ggggagccat
     2521 gcagggagca tgcggcaaac aaaaaggaag gccgccgcgg atgcggcggc ttcatcgttc
     2581 atctcatctt cttctgttga cagctgacta gatagctgag cctacaatga gttgataagc
     2641 ttcaaacatg ttatagcaac tgctgctgta tggctgcaca actacgtaat accttgtcag
     2701 tggtgtgagc tgcaacgtga ggtccatgag agttcccttg ttgcccaggt cctgattggt
     2761 tatctccttt cctgttgcat ccacgactct gagtctgaag tcggccgtct gtgtgcaggt
     2821 tatccgcacc ttgcccaaga ggggtgcagt gaactgccac caatcctcat cagtgcctgt
     2881 tgcgatcacc ccggttaccg gctgtcctag ctgaatgctc ttggcctcat tggcggaatt
     2941 attgggttct gcggggtcgt acagtgcagg cttgactgtg atcttcatgt tgtaaggggt
     3001 gtgcatgctg tatatgttgg tgttatatcc ccgcatcaat acgtaatacg ttccaggctg
     3061 gaatatgtca tgggtcagga ccatcttgtt ccctggatgg ccgtagtcgt cgccgaggag
     3121 tactgtcttg ccgtcgcggt caatgaggga gagatgcaag tcaactggtt ggtcagattc
     3181 tgccacaacc tgagatggtc cagcgatgtc cactttgtag taatctaggt cgcccgggcg
     3241 ggatatgaac gctgcgaagg tttgcccaag aggcggcact gcggcctgcc caggcgtgtc
     3301 gttcggctcg aatctgtcag tacctccgtg cgtaacggtt atggtcatgg tgtagggagt
     3361 gtgcatttcg tatatgtcct tgttgtaccc gcgcatcact acgtagtagg ttcccgggtc
     3421 gtatacgtcc ccttcaagaa gcatgttagt tcctttgctt cctaagtcgc cgccgtagag
     3481 ctcctttgtg ccggaactgt ccacgatgga gaggtagtag tcggccggct gtgtagacgt
     3541 cactttgatg gtggcgggtc cagaaacact gacgctgtag taatcgatgt ccccgggcct
     3601 atagatgaag gaatcgaact gccttccgga gacgactgcc gatgccaggt taacgttgtc
     3661 gttgggctcg aaggcatcac gggcttcccg aaactctgcc ctcagggtgt aaggggtatc
     3721 agacttgatg ttgcctgagt atccgcgtac gagtatatag tagttgcctg gttcaaaaac
     3781 gtcggcattg agcaccattg ttgtcccgct gcttcccaga tcatctccgg cgatctgact
     3841 tcccgcttca ttcagtattg ccatgaagaa atctgcgggc tgtgtacatg tcacgtggaa
     3901 agttccatgt gtggtcaccg taaccttgta catgtcaata tcaccatctg tggagatggc
     3961 gcctgtgaca tttgtattaa cggctattgg atttgcgctt gcgatattat agttgggttc
     4021 acgttctgct tgcaagtagc ttggctcgag cgctggcggg actgcgggtt ggcttggctg
     4081 tgttgtcgga agctgagccg atggcgaccc cgcggaaggc ttcgttaaca ctgcatgcca
     4141 gggaagacct tttccgcctg tgatattgca gtcgaaggtt ccttctatga tcaagtttcc
     4201 tgatgcatcc cgcgagaccg tgccacgata cacctgattg ccgctgccga ctattcgttc
     4261 gaaggcgact gagccgtcag gtgacactct gccgtttgcc aaggtgtctc cgaagacgct
     4321 acccgagagt gttgtaccgc tctgttcgat cctgatctca ccgccgaatc cgttgaggtc
     4381 agcgctccat atgccggaga gttcgaacaa tggacccgat atcggcgtcg aagggatggc
     4441 aatagctggg ggcggtacct gctgcccaac ctgggtgctg cccatggatg atttgctcac
     4501 ttgtatcagc atcacgtcag atcgaggaca ggccatgaga tatccgtcgg tcagtcggaa
     4561 gacgaaatgg tccactccat caaaggtgaa gaaacctact agatctgcat cgtacgtggt
     4621 tccatctagc aaagtaacgg atgagcctag agcgccagcg gatactgcca acaagcacag
     4681 cgttgccagc gctgtggcaa ggtgagtgcg tagttggtgt ttcacggata agccccccct
     4741 cttgcaattg atagaatagt agttctgcta caaaatgcac tttcctgcat aggtaatagc
     4801 ctgtttggga cggaaattct cgagcgctat ttgggccggt ggcctggcaa gatgttgcac
     4861 aattcgggac tcgagtatgc atgttccatc ttgactgcgg tgcgctttcg cgtctatgcc
     4921 gcagtccaat a
//
LOCUS       DAOSAI010000171         4908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10852_length_4908_cov_3.626005, whole genome shotgun sequence.
ACCESSION   DAOSAI010000171 DAOSAI010000000
VERSION     DAOSAI010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10852_length_4908_cov_3.626005"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLM83_06645"
     CDS             complement(<1..558)
                     /locus_tag="PLM83_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOK71117.1"
                     /translation="MTYNLEDANWTERTELIIGADGVARLAGSSVAVVGLGGVGSACA
                     EALARAHVGKLIFVDGDVVSTTNINRQIIAFTDNIGRPKAEIMVERVRRINPQCEAVA
                     EVCWVDGSNVGDLLRLRPDFVADAIDSVGAKLDLIEACLRSQIPIVSSMGAGNRLDPT
                     AFAIADISKTHTCPMAGVSDRGCAGA"
     gene            745..1626
                     /locus_tag="PLM83_06650"
     CDS             745..1626
                     /locus_tag="PLM83_06650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015733514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOK71118.1"
                     /translation="MAQDIASGSVDVVVTSPPYNLGIEYGTYDDRIDRDEYLEWTRAW
                     TSQVARVLSPKGSFFLNIGSKPSDPWVPFEVAMELRETFVLQNVIHWVKSIAISHEDV
                     GDYPAIREDIAVGHYKPINSKRFINDCHEYIFHFTHNGDVTLDRLAVGVPYQDKSNIN
                     RWTREGSSPIDLRCRGNTWFIPYETITRRSSERPHPATFPPKLPEMCIKLHGLDRCRL
                     VMDPFLGLGNTAIACVRLGMPFVGYEIDRFYFAKSVERVTQAVKEREIIEDDSRVQVT
                     QQSLFPEHDQTEKDASL"
     gene            complement(1616..2563)
                     /locus_tag="PLM83_06655"
     CDS             complement(1616..2563)
                     /locus_tag="PLM83_06655"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOK71119.1"
                     /translation="MFIMDAHCDTLMDIVEGRRRLADRDKGGQLDLARMMEAGVSAQV
                     FAIYVPPQYLPGGGARRALQYVDAFYREIEENSDKLVFATSGRDVEEAYEEGKIAALL
                     SIEGGEALDGCIELLRIFYRLGVRLMTLTWSLRNELADGAFESRTGGGLTRFGVEVVE
                     EMDRLGMVVDVSHLSEAGFWDVMEVSKRPIIASHSNSKTLCDHPRNLTDDQARAIAQK
                     GGVIGVNFAGYFLGEERRSLAGVVDHIDHFASLIGPECIALGSDFDGLPDEQLPDELK
                     DVSSLGKVAEELAARGYSESAIQGIMGENLLRAVRAVIG"
     gene            2713..3570
                     /locus_tag="PLM83_06660"
     CDS             2713..3570
                     /locus_tag="PLM83_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOK71120.1"
                     /translation="MPKIKIVTDSTADLGPELIKRYGVEVVPLIVTFDEETYTDGVDI
                     DTHRLFQLVKEKGKLPKTSSPSPETFRSVFEKAIADGSQVIYIGISSQFSASVQSARL
                     VANTFPEGQVHVFDSANLSTGIAQLVLYAHDLAAENKTVPEIMDLLEKARPKVKTCFM
                     IDTLEYMHMGGRCTGIQTLLTSVLKVRPIIEVVDGGMRVAAKIRGSRTKLLDWQLTRF
                     AQDVERGLVEPVRVFLTHTGVHEDALYMADRIRRIMPEMREILETPAGSVIGSHCGPG
                     TIGILYMLK"
     gene            3670..3894
                     /locus_tag="PLM83_06665"
     CDS             3670..3894
                     /locus_tag="PLM83_06665"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HOK71121.1"
                     /translation="MAQRIGAILFQIGGMMTVIGFILVRHPKAFSWFGHLPGDFMTET
                     VIAPIASMLVISLGLSALSWIFGALLRLIR"
     gene            complement(3930..4676)
                     /locus_tag="PLM83_06670"
     CDS             complement(3930..4676)
                     /locus_tag="PLM83_06670"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOK71122.1"
                     /translation="MLGLGAKYLDELRKLIDRIEKTQSENIAEAADAIVDSVSQGGAV
                     HVLDTGHMLMNELFGRAGGLMMPRPVNIKMEVDNPGPARPGKTKEMVYLDEIPGLPEF
                     ILDRSDIYPGDVLIIGSVSGKNALPVGLALLARERGIKVIALTSVAYSSSLEAQHPSG
                     KHLFEAADIVLDNCGVIGDAILEVEGLDTKAIPTSGIAAACLMWTLEAEIIDRMLKRG
                     LTPQVWKSNHLPGAGEFNRKALAETERVGY"
BASE COUNT         1149 a         1457 c         1187 g         1115 t
ORIGIN      
        1 cgcgcctgcg caacccctgt ctgacactcc tgccataggg cacgtgtgag tcttcgatat
       61 gtcggctatt gcgaacgccg taggatcaag cctgtttcca gctcccatcg acgagactat
      121 cgggatttgc gatcttaagc aagcctctat cagatcaagc ttcgccccga cagagtcaat
      181 agcatccgcc acaaagtcgg gacgaagcct gagcaagtcg ccgacattgc tcccgtctac
      241 ccagcaaact tcggccactg cctcgcattg agggtttatc ctacgcactc gttcaaccat
      301 gatctcggct ttgggccgcc cgatattatc agtaaaagca atgatctgtc tgtttatgtt
      361 tgtggtagaa accacgtctc cgtcgacgaa gatgagcttg cctacatgcg ccctagcgag
      421 cgcctccgca caagccgatc ccactccccc gagccccaca acagcaactg atgaacctgc
      481 caggcgggcc actccatcag cgccgatgat cagctcagtc cgttctgtcc aatttgcatc
      541 ttccaagttg taagtcatcc cttccagatc cccggctttt ggtctatcat ggagtctgcc
      601 aggatttatc cgcgccaacg tagaagaacg taatcatcga agcgaggtgt cttaaggttg
      661 agcaaccacg ttgcaactga gatcatcgat attcccaata gcagcgacac cgtcattata
      721 cataatcgcg actgcattga aggcatggcc caggacatag cttcaggatc ggtggatgta
      781 gtagttactt cacccccgta caacctgggc atcgaatatg ggacatacga cgaccgcatc
      841 gaccgcgacg aataccttga atggacccgc gcatggacat ctcaggtggc gcgcgttctc
      901 tcgccaaaag gatcgttctt cctcaacata ggctccaagc cgagcgaccc atgggttcct
      961 tttgaagtcg ccatggagct gagggaaacc ttcgttcttc aaaacgtgat ccattgggtc
     1021 aaatcgatcg ctatctccca tgaagatgtc ggagactacc cggcaatccg cgaagatata
     1081 gcagtggggc actacaagcc cataaacagc aagagattca tcaatgattg ccacgaatac
     1141 atattccact tcacccacaa cggagacgtt accctcgatc ggctggcagt gggggtgcct
     1201 taccaagaca aaagcaatat caatcgctgg acccgagaag gcagctcgcc aatcgatctg
     1261 cgctgccgtg gaaacacatg gttcattcct tatgagacaa taaccaggcg gagcagcgaa
     1321 cgcccgcatc cggccacatt ccccccgaaa ctcccggaga tgtgcataaa gctgcacggc
     1381 ctcgaccgat gcagactagt gatggaccca ttcttgggct tgggcaatac cgctattgcc
     1441 tgcgtacgcc tgggaatgcc tttcgtcggc tacgaaatcg atcggtttta cttcgccaag
     1501 agcgtagaac gcgtaactca agccgtgaaa gaaagggaaa taatcgagga tgacagcagg
     1561 gtccaggtca cccagcaaag cctttttccg gagcatgatc aaaccgaaaa agacgctagc
     1621 ctataactgc acgaactgcc cggagcaggt tttcccccat gatcccctgt atcgctgatt
     1681 cagagtagcc gcgggcagcc agttcttccg ctacctttcc aaggcttgat acatccttga
     1741 gctcgtcagg gagctgctca tcgggcaggc cgtcaaaatc cgaacctagc gctatgcact
     1801 ccggtccgat taggcttgcg aaatggtcta tgtgatcaac aacgccggca agggaccttc
     1861 tttcctctcc taggaagtat cctgcgaagt ttacgccgat aaccccgccc ttttgggcta
     1921 tcgctcgcgc ctgatcgtcc gtgaggtttc tcgggtgatc gcagagggtt ttcgagtttg
     1981 agtgcgaagc gattataggc ctcttcgaca cctccatcac atcccaaaac ccggcttccg
     2041 acaggtggga tacgtctaca accatcccga gcctgtccat ttcctccact acctccacgc
     2101 caaatcttgt gagcccgcct cctgtccgac tctcgaaagc cccgtccgca agctcgtttc
     2161 tgagactcca agtcaacgtc atgagcctta cgccaagacg atagaaaatg cgcaggagct
     2221 ctatgcaccc atccagcgcc tcgccgcctt ctatcgacaa tagcgccgct atcttgcctt
     2281 cctcatatgc ctcttctacg tctctgccac tcgtggcgaa gaccagcttg tcgctgttct
     2341 cctcgatctc cctgtagaac gcatcaacgt actggagagc cctcctggca cccccgccag
     2401 gcaaatactg aggaggcaca tatatggcga atacctgcgc cgaaactcca gcttccatca
     2461 tgcgcgccag gtcgagctgg ccgcccttgt cgcgatctgc caacctgcgg cggccttcga
     2521 ctatgtccat gagcgtatcg caatgtgcgt ccattatgaa catgtactgc acctccaacg
     2581 cttgtgcaat caatacatat tgagtatacc actacaaccg cccaccagca ttgtcccgtt
     2641 ttggctgaag tgctaatatt gggcatgagc cgaatagtgc acaaaagtgt tcgcccggga
     2701 ggttgatcat gcatgccgaa aatcaagatt gtcaccgact ctacagcgga cttaggcccg
     2761 gaactgatca aacgctatgg cgtggaggtc gtcccgctca tagtcacatt tgatgaagaa
     2821 acatacaccg acggagttga tatcgatact caccgcctgt ttcagcttgt caaggagaaa
     2881 ggaaagcttc ccaaaacctc gagcccgagc cccgaaacct ttagatcggt ttttgaaaaa
     2941 gccatcgccg acggaagtca ggtaatctat ataggcatct ccagccagtt ttccgcttct
     3001 gtccaaagcg ccaggctggt ggcaaacaca ttccccgaag ggcaggtgca tgtgtttgat
     3061 tcggccaacc tgtccacggg aatcgcacag cttgtccttt acgcacatga tctagccgcc
     3121 gaaaacaaaa ccgttcccga gatcatggac ctcctcgaaa aagcacgccc caaggtcaaa
     3181 acgtgcttca tgatcgacac tctagagtac atgcacatgg gaggcagatg cacggggatc
     3241 cagacgttgc ttacatcagt tctcaaggta aggcccataa ttgaagtagt tgacggaggc
     3301 atgagggtgg cggcaaagat acgtggaagc aggaccaagc ttctcgactg gcaactcaca
     3361 cgattcgccc aagatgtcga gcgcgggctc gtcgaacctg tccgggtttt tctgactcat
     3421 actggggttc acgaagatgc cctgtacatg gcggatagaa ttcggcgcat catgcccgag
     3481 atgcgcgaga tacttgaaac gccggcaggc tctgtgatag gaagccactg cggtccgggc
     3541 accatcggaa tcctttacat gctaaaatag atgcatcctt atccatatac atgagcttga
     3601 tccagagggc aaggcggatg cctgccctct ggatcgtgtc tgaaaaacaa agcttgagga
     3661 gtgatcttct tggctcagcg cattggagca attctatttc agataggcgg catgatgacg
     3721 gtcatcggat tcatcctcgt acgccaccca aaagccttct cctggtttgg acatctgcca
     3781 ggggatttca tgacagaaac tgtaatagcc cccatcgcat cgatgctagt gatcagcctc
     3841 ggcctgtccg cgctctcctg gatattcggc gcgctgctcc gtctgattcg ctgatcgaat
     3901 cggggcttcg cctcttcttc gctcctctgt cagtacccca cccgctccgt ttctgcaagg
     3961 gcctttctgt tgaactcccc cgctccagga agatggttgc tcttccacac ctggggcgtc
     4021 aagccccgtt tgagcatcct atcgatgatt tctgcctcga gcgtccacat caagcaggcc
     4081 gctgcgatgc ccgatgtcgg aatcgccttg gtgtcaaggc cctctacttc aaggatggca
     4141 tctcctatga cgccgcagtt gtcgaggacg atgtctgctg cttcaaacaa gtgtttgccg
     4201 gacggatgct gcgcctcgag cgacgacgaa taggctacgg atgtaagcgc aataaccttt
     4261 atgcccctct cccttgcaag aagcgccagg cctacgggga gcgcgttctt cccggataca
     4321 gaccctatga taaggacatc gccaggatag atatcgcttc tgtcgaggat gaattccggt
     4381 aacccgggta tctcgtcgag atagaccatt tccttggtct taccaggcct tgcggggccg
     4441 gggttgtcca cttccatctt gatgttaaca ggtctcggca tcatcagccc gcccgcccgg
     4501 ccaaacaact cgttcataag catgtggccc gtgtccaaga cgtgaacagc tccaccctga
     4561 ctcacggagt ctacaatagc gtctgcagct tcagcaatgt tttctgactg tgtcttctca
     4621 atcctgtcta tcagctttcg cagctcatca aggtacttcg cgcccaaacc gagcattcca
     4681 atcagtcctc ccaacctttg gtcaatgaag ggcctcgcag cccagtaacc agcgtccaac
     4741 agcggctagc cttccgccta tcaaaacgat ttgatcttgg atctatactg tttaagcaat
     4801 acctcgttat gttccaatcc gcctggtacg ttagagctca tgaagatagg cgggtcgatt
     4861 ccgcgttcca cgagtctctc gatgacccgc actacaacag tgttgagt
//
LOCUS       DAOSAI010000172         4890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_10886_length_4890_cov_4.004550, whole genome shotgun sequence.
ACCESSION   DAOSAI010000172 DAOSAI010000000
VERSION     DAOSAI010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10886_length_4890_cov_4.004550"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="PLM83_06675"
     CDS             <1..1998
                     /locus_tag="PLM83_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71123.1"
                     /translation="GDAKIAQETSASVALNYSGLEQAMEEQRKQLLAEIGKTTGASDQ
                     KLTAVFGDLKKQSQEIETELGQEISELTARADSLEAFAHRSFASEAQSRADLEAKLTL
                     SLNKISEDQRAYVAQMNELGARVNDVDGRLNALQSETGSRIATLSLRLDDTAKGLAAN
                     KAWMESALAALDNKVSQLSDALDAQRVDTELKLGSMQAYQNGQLSSAIEGERVAREKA
                     DAGLVQQIDALSLSLSKETQERKASDAEIAARQTELESTLNLKIDSAIQTERATRASE
                     LAELMARLESLTSSQDELEQTTALRLAAGIEREKADREKAQAGLAQQVSALSLSLDEE
                     SKAREALGLDMLARQAQLESALTRKIDSAIEGENADRKAAYAELSSKIDAFSLSLGKE
                     LETERAQREAADIAHQEEARRILDEIAAARRDITDIERKTAEADAAIIAALEIQESRV
                     AELGAELARLAADVRTLQEYAAGIEAELLQMSEKTSEDYASQQAALAELAKALDQTSA
                     DLSAAIKANAAEDLKTNGRVLQNTMRIQDVARSLDATNERLGQTDIAVRQAEGSIEEI
                     TARLAEIDSHLASIDRLLAGSVDELAKRFSDDLVVERWEAEMREVKLQSMIEELEARV
                     EKLEGEKAQADAKKPGSAGAIVAVLGVIAAVAAIAIGSGGK"
     gene            2527..2694
                     /locus_tag="PLM83_06680"
     CDS             2527..2694
                     /locus_tag="PLM83_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71124.1"
                     /translation="MEESLNREFIADILTIATIAISLWLTGEKDDTVLEKKCETLPNM
                     GMFRPFGELSK"
     gene            2713..3909
                     /locus_tag="PLM83_06685"
     CDS             2713..3909
                     /locus_tag="PLM83_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOK71125.1"
                     /translation="MRRCQRLGGNLGVQSFRFSATGRPREQKIVVFDQNISRNQWAEI
                     LANCRGHILRELPLVNGVLVLVDEEEIADVQALAQSRSGVVRVEDDIDIELAYEVGKV
                     QWGPRFREDVPWGVKRINIGDASQTGNGVNVGILDTGVDSRHPDLAGNIKGGYSAIAG
                     IDSASDDNGHGTHVAGTIGALQNSIGVLGVAPRANIYAIKVLDAKGSGKLSSLIDGIG
                     WCVEKGIKVVNMSLSSSTENQTFRDMVQSARKAGITMVCAAGNRGPGSNSVGYPAKYV
                     ESIAVAAIDENDKIANFSSRGREISISAPGVDILSTWPGKQYRKSTGTSMAAPHVTGA
                     VALLFEADPSLSPADVKSLLQASADRLDGVSSDEQGAGVLNVSQALSRVSKKRVREVC
                     VMGVAT"
     gene            3906..4403
                     /locus_tag="PLM83_06690"
     CDS             3906..4403
                     /locus_tag="PLM83_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71126.1"
                     /translation="MRIPTDPDSIGLIKAMRPFLSERGQAAVDDVLGSVNLVGIYTSV
                     SDVLTKRRGMGHDRPLAFLSSLANMELDPKIVSKAVDSMMEMSKKPESEPERATSPKG
                     PSGPSADDVAKMLQNVMDKASSDPNFAALLGSLAEEGMRSNMLPKLLESLGPEFLPPQ
                     DQPEQ"
BASE COUNT         1202 a         1101 c         1533 g         1054 t
ORIGIN      
        1 ggcgacgcca aaatcgcgca ggagacttct gcttcagtgg cgctcaacta cagcgggttg
       61 gaacaggcca tggaggagca gcgcaagcaa ttgctggcgg agatcggaaa gaccacgggt
      121 gcgtccgatc agaagcttac tgcggtcttc ggtgatctga agaaacagag tcaagagatc
      181 gaaacagagc tgggccagga gatttcggag cttacggcca gagccgactc cttggaggct
      241 tttgcacata ggtcgtttgc ttctgaagcc cagtctcgtg ctgatctcga ggccaagctg
      301 acgctatctc tgaacaagat cagcgaggac cagcgggctt atgttgccca gatgaatgag
      361 ctcggggcgc gggtaaatga cgttgacgga agattgaacg cgcttcagtc ggagaccgga
      421 tcacgtatag ccacgctctc tctcaggctt gatgacacag ctaaaggctt agcagcgaat
      481 aaggcctgga tggagagcgc attggcagcg cttgataaca aggtcagcca gctgtcagac
      541 gctttggatg ctcagcgcgt cgacactgag ctgaagctcg ggtcgatgca ggcatatcag
      601 aacggacagc tttcatctgc catagagggc gaacgtgttg ctagggaaaa ggcagatgca
      661 ggactggtgc aacagataga tgctctgtcg ctttctctga gcaaggagac tcaagagcgg
      721 aaggcttcgg atgcggagat tgcggcccgc caaactgagc tcgaatcgac gctcaatctc
      781 aagatagaca gcgcgatcca gactgagcgt gccacgcgag cttccgaact tgccgaactc
      841 atggcgaggc tggagtcttt aacatcgtct caggacgaat tggagcagac aactgctctc
      901 agattggctg cggggataga acgtgaaaag gccgataggg aaaaggcgca ggcaggactg
      961 gcgcaacagg taagcgcctt gtctttgtcc ttggatgaag agtccaaagc gcgagaggct
     1021 ctgggcctcg atatgttggc ccgccaagct cagcttgaat ctgcgctaac tcgcaaaatc
     1081 gacagtgcga tcgaaggcga aaacgccgac aggaaggcgg cttatgcgga actctcgagc
     1141 aagatagatg ccttttcact ttccttgggc aaggaactcg agaccgagcg cgcgcagcgt
     1201 gaagcggcag atattgccca ccaggaagag gcaagaagga ttctggatga aattgcggcc
     1261 gcccggcgcg acattactga catagaaaga aagactgcag aagcagacgc tgccatcatt
     1321 gcggccctgg agatccagga gagccgagtc gccgaactcg gtgcagaact tgcacgtctc
     1381 gctgcagatg tgaggaccct ccaggagtat gccgcaggca tcgaggcgga gctgctccag
     1441 atgtctgaaa agaccagcga ggattatgcg tcacagcaag ctgccctggc ggagttggcg
     1501 aaggccttgg accagacatc tgccgatctg tcggcggcca tcaaggccaa tgccgcagaa
     1561 gacctgaaga ccaacggaag ggtgcttcag aacaccatgc gcattcagga tgtggcgagg
     1621 agtcttgatg ctacgaatga acgtttgggg cagaccgata tagcagttcg tcaggccgaa
     1681 ggctcgatag aggagataac tgctaggctc gccgagatag attcacatct agcaagcata
     1741 gacaggttgc ttgcaggaag tgtggacgag ctagccaaaa ggttctcgga tgaccttgtg
     1801 gtggaaagat gggaagccga gatgagagag gtcaagcttc aatcgatgat tgaagagctt
     1861 gaggcgaggg tggaaaaact tgagggtgag aaggcccagg cagacgcgaa aaagcccggt
     1921 tctgccggag ccatcgttgc agtactcggc gttattgccg ctgtagctgc aatagctata
     1981 ggaagcgggg gcaagtagga aactctcgag cgctctaatg ctccttttgg aagcgttttg
     2041 ccttttcttt gaggcggtat acgtagcatg tagcttataa gcaggcgggg gtcgttgagg
     2101 ccccggcctg cttttgatca aagaacgaag attgtctctt caggtcttgg tatcgatcat
     2161 ggcaatggtg catgattctc gcgttaagct gtaatccctg tccccaaact tctcatggca
     2221 accttccaac tccgttctgc gggttgccgc atgtccttgc cacaactgct atgaggcagt
     2281 tgcagcaacg gggcaaggta ctatgatgaa aggtcttttc atcgccattt ctcccttcac
     2341 agaatagaat ctccatgtag tgcccggttt gccaattcaa ccatctgaaa tgctttggcg
     2401 ggtaccggag agaatccgga ttggctttga aagcgcccat ggcatggaac caaaggtacg
     2461 ccaaagtcat aatgtatacc gctttgaggt ttgcaggcgg cgaaaggcgc aaggaggaga
     2521 gtgtgaatgg aagagtcttt aaacagggaa ttcattgctg acatcctcac aatagcaacg
     2581 atagccatta gcctatggct gaccggggag aaagacgata ccgtgttgga aaaaaagtgc
     2641 gagacactgc cgaacatggg gatgtttcgt cctttcggcg aactgtccaa gtagggcagg
     2701 ggaggtagga tcatgcgtag atgtcagagg cttgggggaa atctgggcgt tcagtctttt
     2761 cgcttctctg cgacagggag gcctagggag caaaagatcg tggtcttcga tcagaacatc
     2821 agccgaaacc agtgggcaga gatccttgcg aactgtcgtg gacatattct ccgtgagcta
     2881 ccgctcgtta atggggtatt ggttttggta gacgaagaag agattgcaga cgtacaggcg
     2941 ttggctcaat cgcgttcagg tgtggtgaga gtggaagacg atatcgatat agagttagcc
     3001 tatgaggtag gcaaagtgca gtggggtccg aggtttcggg aggacgttcc ctggggcgtg
     3061 aagaggataa acatcgggga cgcaagtcaa acgggaaatg gtgtgaatgt agggatatta
     3121 gacacaggcg ttgactcgcg ccacccggac ctcgctggga acataaaggg cggctatagc
     3181 gcgattgccg gaatcgattc cgcctcagac gacaacggac atggtaccca tgtagcaggg
     3241 acaataggtg cactgcaaaa cagtattggg gtgcttgggg ttgcgccgag agcgaatatc
     3301 tatgcaatca aggtcttgga tgcgaaagga tccgggaagc tgtccagtct gatcgatggc
     3361 atcggttggt gtgtggaaaa gggtatcaaa gtggtcaaca tgagcctcag ttcgagtact
     3421 gagaaccaga catttaggga catggttcag agtgcgcgca aagcaggaat aacaatggta
     3481 tgtgcagcag gcaatcgcgg gcctggcagc aatagtgtgg gctatccggc gaagtacgtg
     3541 gaatccatag cggttgcggc tattgatgag aatgacaaga tcgccaactt ttcaagcagg
     3601 gggcgggaga tctcgatttc ggctccggga gttgacattc tctcaacctg gccgggcaag
     3661 caatacagga agagcacggg gacatcaatg gcggcgcccc acgttacagg tgcagtggcg
     3721 ctcctttttg aggcggatcc gagcttgtct ccggcagatg tcaagtcatt gctgcaggca
     3781 agcgccgaca gactagacgg agtcagttct gacgagcaag gtgccggagt tctcaacgtc
     3841 tctcaggccc tgtcaagagt atcgaaaaaa cgggttcgcg aggtttgcgt tatgggggtg
     3901 gcgacatgag aattccgacc gatccagaca gtatagggct gataaaggct atgcgtcctt
     3961 tcctgagcga gagggggcag gcggcggtag atgatgtcct gggttctgtt aacttagtag
     4021 gcatttacac ttcagtcagc gatgtgttga ccaaacggcg cggcatgggg cacgatcgtc
     4081 ctttggcctt cctgtcgagc ctggctaata tggaactcga tcctaagata gtttcgaagg
     4141 cggtggacag catgatggag atgtcaaaaa agcctgagtc ggagccggaa agggcaacca
     4201 gccctaaggg gccctctggc ccgagtgccg atgatgtggc caaaatgttg cagaacgtga
     4261 tggataaggc gtcttctgat ccgaattttg ctgcgcttct tggctcgctt gcggaggaag
     4321 gaatgaggtc aaacatgttg ccaaaactcc ttgagtcgct tggaccggaa ttcctgccgc
     4381 cacaggacca gcctgagcag tgataggggg tgttcgggtg aatctgggcg atatatttgg
     4441 gccaggcagt gcattgggaa aactccttcc gctcttgatc atgttgatca tctttctttt
     4501 catagaatgg tggttctagc gataaaccga atcgggggtg acttcattgg tcgaggggtt
     4561 tcctgaaact aaagggcaat ggttcttctg ggggtttcca tggtggtggt ggttgatact
     4621 ggctgccgta gttcttgtcg tgatcttcgt cttcttggtt tagggagcaa attgtgatgg
     4681 aatgatgccg acggcttgca ttgcagggtt tcgaatagtc tggtccatgc tgagcgacaa
     4741 ggtctcatga ggaagtagtt gtggatagtg aaacacgctg ttgtcctctg ccagtgagtt
     4801 ccaagaggat gatcgcgttg gggtaaaaca tgagttgcgc catggcggtc tacacacgat
     4861 ccgaaggagg gcgccggcat gcgctggcag
//
LOCUS       DAOSAI010000173         4834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11049_length_4834_cov_3.536095, whole genome shotgun sequence.
ACCESSION   DAOSAI010000173 DAOSAI010000000
VERSION     DAOSAI010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11049_length_4834_cov_3.536095"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1296)
                     /locus_tag="PLM83_06695"
     CDS             complement(58..1296)
                     /locus_tag="PLM83_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOK71127.1"
                     /translation="MKAIQFEGSVPRYAYSTIFGPLSKRAYYDGLSNIVFRNVSPPSL
                     PNQEWVRVKTKYAGFCGSDKNLIQLHDSPATSPFASFPFTIGHENCGYVVEMGDIAAE
                     RLADKTPLGSRVLVDPLLSCAQRGIDPVCPACARGDYSLCYNFTEGTVSPGFSIGSCR
                     DTGGGWSEEFVAHYTQLIPIPDNVSFEDAVLVDAFCSSLHPVMRNFPSDDSTVLVIGC
                     GVIGLSAIAAIRALGGKARIVALAKYPFQGEAAKECGADEVVYMSRSSDYYTSLAKAL
                     DAKLLDPMLGKKVVLGGADIVYDCVGSSTSIDDALRFTLPHGTMVLVGLASFPKGVDW
                     TPIWLKEVQVRGSFWCSTEKFEGRRMRTYEVAVELLRTERLKLAHFLTHKFALEDYKQ
                     AIEANLNAGHTGLIKSVFEF"
     gene            complement(1293..2489)
                     /locus_tag="PLM83_06700"
     CDS             complement(1293..2489)
                     /locus_tag="PLM83_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOK71128.1"
                     /translation="MSVFQDRIDTFIETYNNLKMSDRYYYLQQVDELDGAHVHIGGRR
                     MVMFSSYSYLGLLKHPKVQERAMEAMDHFGTGTHGVRILAGTTTEHVKCEAKLAEFLG
                     QEDAIAYSSGYVANLSTIQTLLGRNDVVITDKLSHASIIDGCLLCNATFQRFKHNDMD
                     DLKRLLEAGKDKYDGKLVIVDAVYSMDGDVCPLPELVEICRQYGAWLMVDEAHSLGVL
                     GETGHGILEYFHMDPHDVEMLSGALSKTLPAAGGFVAGKHDLIEFLRHNARGFVFSAA
                     IPPASAAAITAALEVIDEEPQLLAAVRRNIDRFVGGLNALGYDTLNTKSCVIPIIIGQ
                     EEPTLELTARLHRDGMFVSPILHPAVPVNTCRLRANVTAAHTDEDIDFALGLLEKHGK
                     DMGLIK"
     gene            2580..2813
                     /locus_tag="PLM83_06705"
     CDS             2580..2813
                     /locus_tag="PLM83_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71129.1"
                     /translation="MAEIVTVEMGKLTLTVWGWQDYMRFAGARGRAIMAIAISVATMN
                     NQQHRVRVSVLGSLGSPNAQAATNPPNNPPIWA"
     assembly_gap    2853..2862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2863..3332)
                     /locus_tag="PLM83_06710"
     CDS             complement(<2863..3332)
                     /locus_tag="PLM83_06710"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOK71130.1"
                     /translation="MSYPERPLSVQRTAPATYTLLVINAIMFGVLEAAGGSQNPFVLL
                     KWGAKFAPAIRAGEAWRLATSVFLHSGLLHLAANSYSLYNLGNVTERLLGTRRFTIIY
                     FLSGIWGSIISTYLSDPMVLGVGASGAIFGLAGCLFYFGVRYPKHFAVIAGSRF"
     gene            4242..>4834
                     /locus_tag="PLM83_06715"
     CDS             4242..>4834
                     /locus_tag="PLM83_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK71131.1"
                     /translation="MGDSGWASLSDGELARMAATGSQDAYSELVRRHSEKLLNLAWRM
                     LGSREEAWDAVQETFLSGWQSIGSYRGDAQVYTWLRKILMNVVFGKSRSGGRIQMVSL
                     DEPAKLEGFEIELEVADASLDPQEVVGRMEMIDEIRLAVAELPIIYRSVFVLREAELL
                     SYAEIAKTLGISEGTVKSRLNMARRLLRAKLAEEVAAA"
BASE COUNT         1052 a         1205 c         1411 g         1156 t
ORIGIN      
        1 ctttattcac cggctgtttg tcctcatcat gccggaaaac ccaagcccgg gcagtttcta
       61 aaactcgaag acggacttga taaggcctgt gtgtccggca ttgaggttgg cctcgattgc
      121 ttgtttgtaa tcctcgagcg caaatttgtg tgtgagaaaa tgggccagtt tcagtctctc
      181 tgtgcgcaac agctctactg ctacttcata cgtgcgcatc cggcgtcctt cgaatttctc
      241 cgtggagcac cagaaggatc cgcggacctg tacttctttt aaccatattg gagtccagtc
      301 cacgcccttg gggaaagacg ctagccccac gaggaccatg gtaccgtggg ggagggtgaa
      361 ccggagggca tcgtctatgc tagtggagct ccctacgcag tcgtagacta tgtcggcgcc
      421 gcccaaaacg actttcttgc cgagcatcgg gtcgaggagc ttggcgtcga gggcttttgc
      481 aaggctcgta tagtagtctg atgagcgact catgtacact acctcgtccg ccccgcattc
      541 ttttgcggcc tctccctgaa atgggtactt ggccaatgct acgatgcggg ctttgccgcc
      601 caaagcccgg atggcggcga tggccgacag acctattact ccgcagccta tcacgagaac
      661 ggtcgagtcg tcgctcggga aattcctcat aaccgggtgc agggaggaac aaaaggcgtc
      721 cacaagcact gcgtcctcaa aggaaacatt atcaggaatg gggatgagtt gtgtgtaatg
      781 ggctacgaac tcctcgctcc agcctccgcc ggtgtcgcgg catgagccga ttgagaagcc
      841 gggggagacc gttccttcgg tgaagttata gcacagggaa taatccccgc gtgcgcaggc
      901 ggggcagaca gggtctatcc cccgctgtgc gcaggagagc aatggatcca caagaacgcg
      961 ggagcccaaa ggcgtcttgt cggccagcct ctctgcggcg atatctccca tctccactac
     1021 gtatccgcaa ttctcgtggc ctattgtgaa cgggaacgat gcaaatggcg aagtggccgg
     1081 cgagtcgtgg agctgaatga ggttcttgtc gctcccgcag aacccggcgt acttagtctt
     1141 caccctcacc cattcctgat tgggcaggga agggggagag acgtttctaa acacgatatt
     1201 cgaaagccca tcgtagtaag cccttttcga caggggcccg aatattgtgg agtaggcata
     1261 cctaggaact gagccttcaa actgaattgc tttcatttta tgagccccat atctttcccg
     1321 tgcttttcga gaaggccgag ggcgaagtct atgtcttcat cggtatgggc ggcggtgacg
     1381 tttgccctca gccggcaggt gttcacgggc accgccggat ggagtatggg agatacgaac
     1441 atgccgtccc tgtgaagtct cgccgtaagc tctaaggtcg gttcttcctg tccgattatt
     1501 ataggtatga cgcaagactt ggtgttaagg gtgtcatatc ccaaagcatt gaggccgccg
     1561 acgaatcggt cgatgtttcg gcggactgcg gccagaagct gtggctcctc gtcaatcact
     1621 tccagcgccg ctgtgattgc tgcggctgac gccggcggta ttgctgcgct gaagacgaac
     1681 ccgcgggcgt tgtggcggag gaactcaatg aggtcgtgct tgcctgcaac gaagccgcct
     1741 gcggccggca gggtctttga gagagctccg ctcaacatct ccacgtcgtg ggggtccatg
     1801 tggaaatact cgaggatgcc gtggccggtt tcgcctagga cgcccaatga atgggcctca
     1861 tcgaccatga gccatgcgcc gtactgccga cagatctcga cgagctcggg aagtgggcag
     1921 acgtctccat ccatgctgta gactgcatcc actattacga gtttgccgtc gtacttgtcc
     1981 tttccggcct cgagaagtcg cttcaggtca tccatgtcgt tgtgcttgaa acgttggaat
     2041 gtggcgttgc acagtagaca gccatcgatg atgctagcgt ggctcagctt gtctgtaatg
     2101 accacgtcgt ttcttcccag cagagtctgt atcgtgctga ggtttgcgac atatccagag
     2161 ctgtaggcta ttgcatcctc ttgacctaag aattcggcca gttttgcttc gcacttcacg
     2221 tgttcggtgg tggttccggc tagaattctg acgccatgtg ttcctgtccc gaaatggtcc
     2281 atggcttcca ttgcccgctc ttgcactttg gggtgtttca gaaggcccag gtagctgtag
     2341 gatgagaaca tcaccattcg ccttccgcct atgtgcacgt gcgcgccgtc caattcgtct
     2401 acttgttgaa gatagtaata cctatcactc attttcagat tgttgtaggt ttcgatgaag
     2461 gtgtctatgc ggtcttgaaa tacgctcacg cttaaccctc cctgcctgtg ttcacaaaaa
     2521 tgtgcacaca cacatattac taccccggat gtgtggtgtc aacgcaggat ggacacggct
     2581 tggctgaaat tgttacagtt gaaatgggta aattgacgct gactgtgtgg ggttggcagg
     2641 actacatgag gttcgccggg gcgagggggc gcgctatcat ggctattgca atcagtgtgg
     2701 ccactatgaa taaccagcag cacagggttc gcgtttcagt tcttggatcg ttgggaagcc
     2761 cgaacgcgca ggcggctacg aatcctccga ataatcctcc gatatgggcg tagttgtcga
     2821 tgagggggtt tgtgaagcct atgatcaggt tgnnnnnnnn nnatgaacct tgatcctgct
     2881 ataacggcaa agtgtttggg gtacctcacc ccaaaataga agaggcatcc ggcaagcccg
     2941 aatatggcgc ctgaggcccc tacacccaac accattgggt ctgaaaggta tgtgctgatg
     3001 atcgatcccc atatgcccga gagaaagtag atgattgtga atcgtctcgt ccccaagagt
     3061 cgttctgtta cgttccctag gttgtagagg gaatacgagt tcgcggcgag gtgaagcaat
     3121 ccggaatgaa gaaagacgga agtcgccagt cgccaggctt cgcccgcgcg gatcgccggc
     3181 gcgaatttgg cgccccattt caggagcacg aacgggtttt gtgatcctcc ggctgcttcg
     3241 agcacgccaa acattatcgc gtttatcacg agaagcgtgt aggtggctgg cgcggttcgc
     3301 tggactgaca gcggccgttc cgggtagctc attcgtatcc ctcccaacgg accctaatgc
     3361 aaatagtata cgatatgcgc catgtgcatc aagataccgc atgcgaacgt tcggcgcatt
     3421 tccggcaatc tgtccgtaat atggttgtct ccggcatcgg gaaggagcaa caaaactgcc
     3481 gttaaccgga gtgggcggcc cgctcaatcg ataaatgggg cgaatttatg ttgatataga
     3541 taaaaaatcg tacttgtttc gaactttttt gctccaattg catctatagt agtagagccg
     3601 gggcaatgcc cagacgaatt ccatatagct gagccaccca gaattgtatt tacgctttga
     3661 aagaagtaag gttaccacca atagacatac atgcatcagg agaaggcagc aagatcagtt
     3721 tgcgtaacat acctcctagg aaacgcagca ggtttggata ctgaaaaggc gcggggatgc
     3781 aattatctgg cgggggtagg agcatatcct tgcaggcaag ttgcgggtgt aagggatgta
     3841 gttggttcag catggcacgt ctggcagtta gcctcggcta ttcttttccc gagatcaaaa
     3901 ggtggatgtc gccacgctcg actccagtat cgcataattg atacaccaaa tagtagtgag
     3961 ccggcaggct tgcagggtac tgcggcctta tggcgaatcc aaaatggtgt agtgctgggc
     4021 gatgcaatcc gccaatttgc atatttgcat ccagatggcg ggggccaggt tggggcttgt
     4081 gtttcggcag gtagttgccg gtattggcaa cgcccccgtt gctcaattat gccccctgtc
     4141 gcctgagaaa gccggcggct tacagggctt ctgaaaacgg ggcccaagag gagtcgcggc
     4201 tgaagtcgct ttcggcctgt tcatctcggg aggtgaacac tgtgggcgat tctgggtggg
     4261 cctctttgtc cgacggagag ctggcgcgta tggcggcaac gggttcgcag gacgcctact
     4321 ctgaactcgt cagaaggcac tcggagaagc tcttgaacct tgcttggagg atgttaggct
     4381 ctcgggagga agcgtgggac gctgttcagg agacttttct gtctggctgg caaagcatag
     4441 gaagttacag gggagatgcc caagtctata catggctacg caagatactc atgaacgtgg
     4501 tttttggcaa gtccagaagt ggcggtcgca ttcagatggt atcgctggat gaacctgcaa
     4561 agctcgaagg cttcgagatt gagctggaag tcgctgacgc tagcttagat cctcaagagg
     4621 ttgttggacg tatggaaatg atcgacgaga tccgccttgc tgtggctgag ctgccgataa
     4681 tctataggtc ggtttttgtt ttgagagagg ctgagctgtt gtcttatgct gagatagcga
     4741 agactctggg aatttccgaa ggtacggtga aatccaggct gaacatggcc agacgattgt
     4801 tgcgggcaaa gctagctgag gaagtggccg ctgc
//
LOCUS       DAOSAI010000174         4811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11136_length_4811_cov_3.060345, whole genome shotgun sequence.
ACCESSION   DAOSAI010000174 DAOSAI010000000
VERSION     DAOSAI010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11136_length_4811_cov_3.060345"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PLM83_06720"
     CDS             <1..1439
                     /locus_tag="PLM83_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK71132.1"
                     /translation="HSSQNEQDNRNYAKAAKLPLIEPSNSQEALDFVKVAFELSEAYS
                     TPVLFRITTRLAHGKSVVTVGRRVDVKPREYVRDTSRFVLMPAQARTRHGVLEDRLIQ
                     LEDWACTTRLNRVEWGSRKLGIITSGVAYHYAHEVFGEDASYLKLGMSYPFPKKLVED
                     FAAQVDHLVVIEELDPFIEDAVRAMGIEAEGKALIPRLGELDSAIIAEAFEKADSKSP
                     PPEDVTGRPPMFCAGCPHRGVFYELGQLPVTITGDIGCYTLGSLPPFAATETCICMGA
                     SISAGLGFQKVFNMLNPADPKRTIAVIGDSTFYHSGMTGLLDAVYNDVGLITIILDNR
                     ITAMTGHQENPGSGYNLSGQSAPQARPEEIAKALGVEQIATVNPYDLTEVKKALDNAL
                     ADPHPSVIVSRAPCALLKREAVRRPPYFVDQDACRKCKACLKVACPALYVEDGEVHID
                     NASCVGCGVCAQVCRFGAINQTEKTGEN"
     gene            1439..2017
                     /locus_tag="PLM83_06725"
     CDS             1439..2017
                     /locus_tag="PLM83_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOK71133.1"
                     /translation="MANTTSIMLAGVGGQGTILASRILAQLLLDKGYDVKMSEVHGMA
                     QRGGSVTTQVRYGEQVDAPTIAPGQADFILSFEAMEAARTAHFLKPGGTIVANTRRIP
                     SLPILIGKEEYPDDIDERLAERGIDVRWVDAYSIAEELGNTRVENVVLLGAFSTVLPF
                     TEGAWKKAIAQVVPPRHVELNVVAFQRGSRLA"
     gene            2020..2337
                     /locus_tag="PLM83_06730"
     CDS             2020..2337
                     /locus_tag="PLM83_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HOK71134.1"
                     /translation="METPIALTVAILGITGAASLIFGYLIGHKNKFDLINGYTEEKML
                     DAAKFAQWMRLCYNIMGGVLIGASALLVATKAVLAIVIACTVIVTVVLAVMTLGARRY
                     YRI"
     gene            complement(2478..3839)
                     /locus_tag="PLM83_06735"
     CDS             complement(2478..3839)
                     /locus_tag="PLM83_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017247588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOK71135.1"
                     /translation="MISGPDLFDYAAEKRGGGDRVPLAARMRPRTIDEILGQDHILGP
                     GKLLRRAIEADKLGSIILYGPPGSGKTTIAEVIALTTKADFVRLNAVTAGVADIRQVV
                     ATARDRWKLQGVRTILFIDEIHRFNKAQQDALLPSVEDGTVTLIGATTENPFFDVNPP
                     LVSRSRIFKLEPLGVDDVRCLIARALDDSERGLGYLNISIDDDALDHIASFANGDART
                     ALNAIEVAALSTPAGEDGRIHITLEVAEDSIQHRALRYDGGGDMHYDVVSAFIKSMRG
                     SDPDAALHYLARMIESGEDPRFIARRLMIHASEDVGMADSQALLVASAALAAVEKVGL
                     PEARIILAHATIYIATAPKSNSVIRAIDAATDDVRTRSISDVPLHLRDASYRGASTFG
                     HGVGYKYPHDYSGAWVSQQYMPEGLEGRRYYEPGENPNEARVRQRMAEMKKGCDGSDD
                     RLR"
     gene            complement(4204..4385)
                     /gene="ssrS"
                     /locus_tag="PLM83_06740"
     ncRNA           complement(4204..4385)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLM83_06740"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4505..>4811)
                     /locus_tag="PLM83_06745"
     CDS             complement(4505..>4811)
                     /locus_tag="PLM83_06745"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOK71136.1"
                     /translation="DRPIDIYFVSLGEAARERAFELAFNMRSCGISADYDMLDRGLKA
                     QMRFAAKVGARFVAIIGDEELATDMVTLKAMATGDQELVLQAELASKVLEAGKAGME"
BASE COUNT         1117 a         1358 c         1306 g         1030 t
ORIGIN      
        1 gccacagttc acagaatgaa caggacaata ggaactatgc aaaagctgcc aagcttccgc
       61 tcatcgagcc atccaacagc caagaggcac tggatttcgt aaaggttgca ttcgagttga
      121 gcgaggccta cagcacaccg gtcttgttca gaataaccac aaggctagcc catggcaaat
      181 cggtcgtgac cgttggccgc cgtgtggacg tcaaaccgcg agagtacgtc cgagacacgt
      241 ccagatttgt tctaatgccg gcacaggcga ggacccgcca tggtgtcctt gaagaccgcc
      301 tgattcaact ggaggattgg gcatgcacca cccggctgaa tcgggtggag tggggttctc
      361 gaaaacttgg catcatcacg agcggagtag cttaccacta cgcgcacgag gtgttcggcg
      421 aggatgcttc ctacttgaag ctgggcatga gttatccatt ccccaaaaag ctagtggagg
      481 acttcgccgc acaagttgat cacctggtcg tcatcgaaga actcgatccg ttcattgagg
      541 acgccgttcg cgccatggga atcgaagcgg aaggtaaagc cctcattccc cgccttggcg
      601 aacttgattc cgctatcata gccgaagcat tcgaaaaggc cgacagtaaa agtccgccgc
      661 ctgaagatgt tactggaagg cccccgatgt tctgcgccgg ttgtccgcat cgcggcgtct
      721 tctacgagct tggacagctt cccgtgacga taacaggtga catcgggtgc tatactctgg
      781 gttcgctgcc cccgtttgca gccaccgaaa cgtgcatatg catgggcgca agcatatcgg
      841 cggggctagg attccagaag gttttcaaca tgctgaaccc ggccgatccc aagagaacga
      901 ttgccgtaat cggcgactcc accttctatc actcaggcat gaccggtctt ctcgatgcgg
      961 tctataacga cgtcggactc ataactatca tcctcgataa ccggattacc gcaatgaccg
     1021 gccatcagga gaatcctggc tctggctata atctttcggg ccagagcgct ccccaagcaa
     1081 ggcctgaaga gatcgccaag gccttaggtg tcgaacagat cgccactgtc aacccatatg
     1141 atcttacaga ggtgaagaag gcactcgata acgcgcttgc agaccctcat ccatcagtga
     1201 ttgtgtcccg agcgccgtgc gcccttctga agcgtgaagc ggtcagaagg ccgccttact
     1261 tcgtcgacca ggatgcatgc cgaaaatgta aggcatgcct caaggtagcc tgtccggcgc
     1321 tgtacgtgga agacggagag gtgcacatcg ataatgcatc ctgcgtcggg tgcggtgttt
     1381 gtgcccaagt atgcagattt ggcgcgatca atcaaacaga gaaaacgggt gagaactgat
     1441 ggctaacact actagcatta tgttggcggg agtcggcggg caaggcacga tattggcaag
     1501 ccgaatcctt gcgcagctcc tcctcgataa aggctacgat gttaagatgt cggaagttca
     1561 tggcatggct cagcgtggcg gaagcgtcac cactcaggtg cgctacggtg agcaagtcga
     1621 cgcgccgacc atagcacccg gacaggccga tttcatactg tctttcgagg ctatggaagc
     1681 tgcaaggacg gcgcacttcc tcaagcccgg aggaacaata gttgccaaca cgagaaggat
     1741 tccctcactc ccgatactca tcggcaagga agagtatccg gatgatatcg acgagcgcct
     1801 tgcagaacgt ggaatcgatg tccgttgggt agatgcgtat tccatcgccg aagaactggg
     1861 gaatacacgc gtggaaaacg tcgtccttct cggggcattt tccacagttc tgccatttac
     1921 tgaaggcgcg tggaaaaagg ccatagccca ggttgtgccg ccacgccatg tggagctcaa
     1981 tgtagttgct tttcagagag gaagcaggct ggcgtaaaga tggagacgcc gattgcgctc
     2041 accgtggcca ttttggggat tacaggggca gcttcgctta tcttcggata tctcataggc
     2101 cacaagaaca agttcgacct gattaacgga tacacagaag agaagatgct cgatgccgca
     2161 aagttcgctc aatggatgcg actatgctac aacatcatgg gaggagtgct gattggagca
     2221 tccgccctgc ttgttgccac aaaggcagtt cttgcaattg tgattgcatg taccgtgata
     2281 gtaacagtcg tgcttgcagt aatgacactt ggcgcacgaa ggtactaccg aatatgagtg
     2341 ctcgtgccat acgcccccgg ctagggcgca agctgcggcc cgagggcgtt attctgtcgc
     2401 tttcctcgcc ttgcccgcct cttcgcaaat aatgcattct atcccgccaa gctagtgatg
     2461 atgaactacc aggaggttta cctcaggcgg tcatcggagc catcgcaccc cttcttcatc
     2521 tcggccatgc gctgcctgac tcgcgcttca ttgggattct cccctggttc gtagtatctg
     2581 cgcccctcaa gcccctccgg catgtattgc tggcttaccc aggccccaga atagtcatgg
     2641 ggatacttgt agcctacgcc atgtccgaag gtggatgcac cgcgatatga ggcgtcccgc
     2701 agatgaagcg gaacgtctga tatggatctg gtcctcacgt catccgtggc agcatcaatc
     2761 gcccggatga cggagttgct cttgggagcc gtagctatgt agatcgtggc atgggccagt
     2821 attatccgag cctcgggaag acctaccttc tccaccgcgg caagagcagc ggacgcaacc
     2881 agcaacgcct gggaatccgc catgcccaca tcctcgctgg cgtggatcat cagacgtcga
     2941 gcaataaacc gagggtcttc gcctgattcg atcatcctgg caaggtaatg cagagcagcg
     3001 tcgggatccg acccccgcat gctctttatg aaggcagaca ccacgtcgta atgcatatcc
     3061 cccccgccat cgtatcggag tgccctatgc tgtatggagt cttcagccac ctccaatgtt
     3121 atgtggatcc ttccatcctc gcccgctggc gtagagagtg cagcaacctc tatcgcattc
     3181 aatgccgttc gggcatcgcc gttggcaaaa ctggcgatgt gatctagggc atcatcatct
     3241 atggatatgt tgagataacc gaggccacgc tctgaatcgt ccagggcgcg cgcaataagg
     3301 cagcgcacgt cgtcgacgcc caaaggctca agcttgaata tgcgggagcg cgagaccaac
     3361 ggcgggttga cgtcgaagaa tggattctca gtggtagcgc cgataagcgt gacagtgccg
     3421 tcttcaactg acggcaagag ggcgtcctgt tgtgccttgt tgaaccggtg gatctcgtcg
     3481 atgaacagaa tcgtacgaac gccctgaagc ttccatctgt cccgagcggt tgccacaacc
     3541 tgcctgatgt cggcaacccc ggcagtcacc gcattaagcc ttacgaagtc cgccttggtc
     3601 gtaagtgcaa ttacctcggc gatcgtggtc ttgcccgagc ctggcgggcc atagagtatt
     3661 atcgatccta gcttgtcggc ctctatcgcc ctccgcagaa gcttccctgg cccgagaatg
     3721 tggtcttgtc caagtatttc atcaatggtg cgaggcctca tcctagccgc aagggggacg
     3781 cgatcgcccc ctcctcgctt ttcggccgca tagtcaaata gatcagggcc tgaaatcatc
     3841 tggctcacct cgggtactgt tcaatgtagc aacttggcat gctcgcccac tggcgcaact
     3901 ccatcataca tcatagaggg caaaggtgga caacgaggca atcggccggc cttctgcata
     3961 tatgttgagg agcaatagat ccgaccccgg gcatggtggt tagcctatgc aggttttgac
     4021 tgcacaacta tgacgttgta cagaggagaa gagtagtctg aagaccagag cacactcttg
     4081 cgctttacgc cgattctgtt cagcgaaaaa caacgatatg gacggatagc aagccaagcg
     4141 ttttcaatcg cagaatatgc tgtcagaaaa gcccaacagg cttaagctgc ccattgggca
     4201 aaaaaggacc ccaccatgcc gtagccgtgg ccgtttctcc cccgcgcccg cgaggtgggt
     4261 gccctcccat cacgtttgcg ttccacgttt tcacgcggcc cacacgccgg tgacagagtc
     4321 caggctccca ttgatgatgt gttggaagta aacagccaga gtgcttacgt gcacagcagg
     4381 gtaatagctc caatcgatgt caagtacatg atatcacacg acctgcggcg tttcaacaaa
     4441 accgatcctg ccaaaaattg aggcaattgg atggaatgcg agccgatacc tgcgcgttag
     4501 cagcttactc catacccgct ttgccagcct ccaaaacctt cgaggccagc tcggcttgga
     4561 gaacaagctc ttgatcgcct gtggccatgg ctttgagcgt caccatatcc gtcgccagtt
     4621 cctcgtcgcc tatgatcgcc acgaaacgtg cccccacttt cgctgcaaaa cgcatctgag
     4681 ccttgagccc gcggtcgagc atgtcgtaat ctgcggagat cccgcaagaa cgcatgttga
     4741 aggccagctc gaatgccctc tcgcgagcgg cctcacccaa ggacacaaaa tagatgtcta
     4801 tcggccgatc a
//
LOCUS       DAOSAI010000175         4783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11221_length_4783_cov_3.343697, whole genome shotgun sequence.
ACCESSION   DAOSAI010000175 DAOSAI010000000
VERSION     DAOSAI010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11221_length_4783_cov_3.343697"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLM83_06750"
     CDS             complement(<1..511)
                     /locus_tag="PLM83_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK71137.1"
                     /translation="MSAHPNSSTLDSALRRQNKQGVGQSPKWGRIACRVVIYAIVILG
                     AVMMALPFVWLILSSFKTLEETMFFPPTFFPKNPTFANFREVFERIPFARYYMNSIIT
                     SVIPTIITVTTCSMAGFGFAKYKFTGRNTLFWIILATMMVPFPATIVPLYAGIPHMRF
                     GLAGYGMQMP"
     gene            complement(508..1440)
                     /locus_tag="PLM83_06755"
     CDS             complement(508..1440)
                     /locus_tag="PLM83_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK71138.1"
                     /translation="MSAKTTTMRRCGGNERKYRWYRFQKASAPYIFILPGVVFYLMVT
                     IVPMIMGFWMSFHNWSIIRPTQPWVGLDNYRKLFADPLFWTSMKNTLVYSIGVVPLQM
                     ILALLIALLLNTGIKGRKFFRLLYYLPVITPMSIAAVIWTWIYHPNLGIFNQIVRFIG
                     LRPQDWLGNPDWAMFSVILVAIWAGIGYRMVVYLAALQGIPRTYYEAAEIDGAGRWDA
                     FKYITLPLLKPTTLYILVTSVISSFQVFGLVNVLTGGGPLNSTQVAVSYIFKRAFGDL
                     QFGLAAAMSFVLFAIILAITLIQWKFLGKEVTYE"
     gene            complement(1595..2863)
                     /locus_tag="PLM83_06760"
     CDS             complement(1595..2863)
                     /locus_tag="PLM83_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK71139.1"
                     /translation="MIKNIRRYGALLLAIALFMGLTCGVSAQKKTTIVHWQHHSPARL
                     ETVKAFAQEFMKENPNVKIEIESVPQDDFFQKLLPAIAAGSGPDTFQIPLGQVPAYAA
                     SGVIQPIDSRVATTRQIESEFITPAIKRMKHNGKYYGFPVDVQTIVLFYNPKLFKEAG
                     LDPERPPQTWDELIKYAQAIHKRDAKGITTQMGVGTGGYGPTVITLMLQNGAPLWNTK
                     TQLPDFNSPQAIEALKFVTDLETTYKVEDAQFGRRNDMFRQGKVGMVFGHPAMIGANK
                     TTAPNLEFRTAEIPAPKAGGSRATLLTSWAYVVSSQAESEICSKWVAYLTSAEAQKRW
                     TLTTGELPSRKALLDLPEYKNDILLAPCMESMKKSVPAPWTARRLDDELIRETYQLIT
                     LKGESPAAALKWLTKEAVKAEKEQQELARP"
     gene            complement(2938..3897)
                     /locus_tag="PLM83_06765"
     CDS             complement(2938..3897)
                     /locus_tag="PLM83_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009590008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK71140.1"
                     /translation="MHAHSYARAVCALPDAKLVGVADEDENRGRAAAEQYGTRYFSDV
                     DQLLGSGVDAVIVCSENARHKEHVVMAARSDVHVLCEKPIAVTVSDGVEMIEACRVAG
                     VSLATAFPVRHVPAIMRAKQMVDEGKVGEVLAVKATNHGRMPGGWFIKRELSGGGAVI
                     DHTVHVADLLRWILKDEFKSVYAEIDTRFHDFDIDDCGTLAFEMEKGIFATLDPSWSR
                     PRNYPTWGDVAMEIIGTDGVISVDAFSQHLVEIGDSQRPVRYRGWGDSSDLALVADFV
                     SAVREGSRPAADGVDGLRALEVALAAYESASCGDVAVLNRVDC"
     gene            complement(3951..>4783)
                     /locus_tag="PLM83_06770"
     CDS             complement(3951..>4783)
                     /locus_tag="PLM83_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK71141.1"
                     /translation="EVDAIDVCLPTYLHKEAVELAAHHGKHVFCEKPIALTVDDANAM
                     ISTCNSNGVKLGIAHVLRYFPEYEMAHDMIASGRLGQIGVVRFSRTGPFPKGWNNWYA
                     DFQLSKGVFVDLSIHDFDFLNWCFGPVERVQARKVSTERPMEYGLAVVRMKSGVIAHV
                     EGSWAHPRPFSYKFEIRGSEGMIEFDSAKAKPLTLFTMPEPLAEGAATGAGAGPGVAV
                     SESPLREKDPYLREIEDFFAYVRGERLPRVLPEDAVEALEVGLAAISSSDTGEVVKVG
                     "
BASE COUNT         1160 a         1382 c         1131 g         1110 t
ORIGIN      
        1 gcggcatctg catgccgtat cctgccaggc cgaaacgcat atgcggaatt cctgcgtaaa
       61 gcggcactat cgttgcagga aacggcacca tcatggtggc aagtatgatc cagaaaagcg
      121 tattcctccc cgtgaacttg tacttggcaa aaccgaaacc cgccatggaa caggttgtaa
      181 ccgtaatgat ggtgggtatc accgatgtta tgatgctgtt catgtagtag cgtgcaaaag
      241 gtatcctctc aaacacctct cggaagtttg caaatgtcgg gtttttcggg aagaacgtag
      301 gaggaaagaa cattgtttcc tcgagcgtct tgaaggatga cagtatcagc caaacaaatg
      361 gcaacgccat catgacggct cccaagatca ctatggcata gatgaccacc ctgcaggcaa
      421 ttcgccccca ttttggtgat tgtccgacgc cttgcttgtt ctgtctacgc agagcggaat
      481 cgagcgtgct gctattggga tgtgcactca ttcgtaagtg acctccttgc cgagaaactt
      541 ccattggatc aacgtgatcg ccagtattat ggcgaacaag acaaatgaca ttgccgcagc
      601 gagtccaaat tggagatctc cgaacgcgcg cttgaatatg tacgaaaccg ccacctgagt
      661 agagttgaga ggaccgcccc ctgtcagcac gttcaccaat ccaaatacct ggaacgaact
      721 gatcacgcta gtaacaagaa tatatagcgt agtaggcttc aaaagaggca gtgtgatgta
      781 tttgaacgca tcccaacgcc ctgcaccatc tatctcagca gcctcataat acgttcgtgg
      841 tatgccctgc agggccgcaa ggtacacgac catgcgatag cctatgcctg cccatatcgc
      901 cacaaggatc actgagaaca tcgcccaatc cggattgccc agccaatctt ggggacgaag
      961 cccaatgaac ctcactatct gattgaatat tccaagatta ggatgatata tccaagtcca
     1021 gatgaccgct gcaatggaca tcggcgtaat cacaggaagg tagtacagca acctgaaaaa
     1081 cttccgcccc tttatccccg tattcagcag gagcgcaatc agaagcgcca atatcatctg
     1141 taacgggact acacctatcg aatatacaag cgtattcttc atagatgtcc agaagagagg
     1201 atctgcaaag agcttccggt agttgtccag tcccacccag ggctgcgtcg gacggatgat
     1261 gctccagttg tgaaagctca tccagaatcc cattatcatc ggcacaatcg taaccatgag
     1321 ataaaacacc acgcccggca atatgaatat atacggagcg ctagccttct gaaaacggta
     1381 ccacctgtac ttgcgctcat tgccgccgca tcgtcgcatt gttgtcgtct ttgcactcac
     1441 agcgcatcca acctccttat tatagccgtg ctgcggcacc taactggaat gtgaggggtg
     1501 cggagtcggg ataccaacac ccagccccgc acccttatac aagctccatt tgcgcacgac
     1561 catgagagtt atggccccgt tgggccacaa catactacgg ccttgccagt tcctgttgct
     1621 ccttttcagc cttcacggcc tctttcgtga gccatttcag agcagcagcc gggctctcgc
     1681 ccttcagtgt tatcagctga tacgtctctc tgatcagctc atcatcgagc cttctggcag
     1741 tccacggagc aggcacggat ttcttcatgg attccataca aggcgcaagc aatatgtcgt
     1801 tcttgtactc cggcaggtcg agcagagcct tccttgaagg aagctccccg gtggtaaggg
     1861 tccagcgctt ctgagcctca gctgaagtca gatacgccac ccatttggag catatttccg
     1921 attcagcctg gctggatacc acataagccc acgaagtcag cagcgtggcg cgcgagccgc
     1981 ctgccttggg cgcaggaatc tcggctgtcc tgaactccag attcggagca gtggtcttgt
     2041 tggcgcctat catggccggg tgaccgaaga ccatccccac cttgccctgg cggaacatgt
     2101 cgtttctgcg accgaactga gcatcttcaa ccttgtaggt ggtttcgagg tctgtaacaa
     2161 acttcagtgc ctctatggcc tgaggagaat tgaaatccgg cagctgtgtc tttgtattcc
     2221 acagcggagc accattctga agcatcagcg taatcacggt aggaccatat ccgcctgtcc
     2281 caacgcccat ctgtgtggtg atgcccttgg cgtccctttt atgaatcgcc tgcgcatact
     2341 tgatcagctc atcccaagtc tgcgggggtc tctcaggatc aagccctgct tccttgaaca
     2401 acttagggtt gtagaagagg actatcgtct gaacgtctac gggaaaacca tagtacttgc
     2461 cattatgttt catgcgcttg atcgccggtg taatgaactc agattctatc tgtcgcgtag
     2521 tagccactcg cgagtcgata ggctgaatga ctcctgaagc agcatacgcg ggaacctgcc
     2581 ccagagggat ctggaatgtg tcagggccag accctgcagc aattgcaggg agaagctttt
     2641 ggaagaaatc gtcttgaggc actgattcga tttctatctt cacattggga ttttctttca
     2701 tgaactcctg cgcgaaagcc tttactgtct ccaaacgtgc tggagaatga tgttgccaat
     2761 gtacaattgt agtcttcttc tgcgccgata cgccgcacgt taaccccata aaaagggcaa
     2821 ttgccaaaag caacgcgccg tatcttctga tgtttttgat cacaatatat acctcccaga
     2881 atatgctgac cgcgcccgag acgtgatcgc agagatgccc tatatcctat tgctcgttta
     2941 gcaatccacc ctattcaaaa ctgcaacgtc gccgcagctg gcagactcat aagccgccaa
     3001 agcgacttcc aatgccctta gaccatccac cccgtcagcg gcgggccgac ttccctcgcg
     3061 taccgcggac acaaagtcag ctacaagcgc aagatccgaa ctgtcgcccc atcctctgta
     3121 acgaacaggc ctctgcgaat caccgatctc gactagatgc tgagaaaatg catcgacgga
     3181 gatcactcca tcggttccga taatctccat cgcaacatcg ccccatgtgg ggtagtttct
     3241 cggccttgac cagctcggat ccagagtggc gaagataccc ttctccatct cgaaagcgag
     3301 tgtaccgcag tcgtcgatgt caaaatcgtg gaacctggtg tctatctccg cgtagaccga
     3361 cttgaactca tctttcaaga tccatcggag cagatcagca acgtgaaccg tatgatcgat
     3421 tacagcgccc ccgccagaaa gctcgcgttt gatgaaccat cctcccggca tgcgtccatg
     3481 atttgtagcc ttaacggcga gcacctcgcc taccttgccc tcgtccacca tctgcttcgc
     3541 tcgcatgatt gcaggcacgt gacgaaccgg aaatgcagta gccaaggaga cccctgcaac
     3601 tctacatgcc tcaatcatct ctactccgtc acttactgtg acagcgatag gcttctcgca
     3661 cagcacgtgt acgtctgaac gcgccgccat aaccacgtgc tctttgtgac gcgcattctc
     3721 agagcaaacg ataacagcat ccacacccga accaagaagc tgatcaacat cgctgaagta
     3781 acgagtgccg tattgttccg ccgccgctcg gccgcggttt tcatcctcat ccgcaactcc
     3841 aaccagtttc gcgtctggca acgcacacac agcacgagcg taactgtggg catgcatgtg
     3901 ggcaaaactg atagcgccta ttctgagttc attcccttga tgttctgcag tcacccgacc
     3961 ttcaccacct ctccggtatc agaagagctg attgccgcaa ggcccacctc aagtgcctct
     4021 acagcgtctt cggggagcac ccgtggcaac cgttcgccgc ggacataagc aaagaagtcc
     4081 tctatctcac gtaggtatgg atctttctct ctaagcgggc tctctgacac agccactccc
     4141 ggcccagctc cagctcctgt cgctgctcct tccgccagag gctccggcat cgtgaacaaa
     4201 gtcagaggct tggccttcgc actgtcgaac tctatcatcc cttcgcttcc gcgtatctcg
     4261 aacttgtagg agaagggtct gggatgtgcc cagctgccct ctacatgagc tataacacca
     4321 gatttcatac gcactacggc aaggccgtat tccatgggcc tttcagtcga aacctttcgc
     4381 gcttggactc gctcgacagg gccaaagcac caattcagaa aatcgaaatc gtgtatggat
     4441 aaatccacga aaactccctt gctgagctga aagtcggcgt accagttgtt ccagcctttg
     4501 ggaaatggac cagtgcggga aaaccgcacg acgcctattt gccccagcct gccagaggct
     4561 atcatgtcat gcgccatttc gtattcaggg aagtaacgca gtacatgcgc tatcccaagt
     4621 ttcacaccat tagaattaca tgttgatatc atggcatttg catcgtccac tgtaagcgca
     4681 atgggttttt cgcagaatac atgcttgcca tggtgcgcgg caagctccac tgcttccttg
     4741 tgcaggtaag ttggaaggca gacatctatc gcatcgactt cag
//
LOCUS       DAOSAI010000176         4758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11305_length_4758_cov_3.173932, whole genome shotgun sequence.
ACCESSION   DAOSAI010000176 DAOSAI010000000
VERSION     DAOSAI010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11305_length_4758_cov_3.173932"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2939)
                     /locus_tag="PLM83_06775"
     CDS             complement(<1..2939)
                     /locus_tag="PLM83_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK71142.1"
                     /translation="MSKASSLEKSLAKKLNRDISCYEKDGAIVLEGNVPTWDHVVAAG
                     KSGAKLGYKGVVNRLTCDGILAMPGPKAPSIRDASLEGERMDVLVIGGGVIGTAILRE
                     LSRWDIRACLIEKEADLAMHQSGRNAGMIHPALAPSPGSKKAQFNSLGNPMIPAIADE
                     LGVPFRYNGMTIIVERRIYGIAYPVLANRARRNRAGEIRFVPPAELQSMEPNVRDDIG
                     GAFFMPQSGIIDPFMLTIAFAENAVENGATVWLNTMAESFIVEHGRILAVVTNRGVLF
                     PKAVVNAAGLWADTVAEAAGDMFFTIHPRHGEMLILDKKKGNLARSTLSMPPVYQART
                     DTKGGGVIPTIDGNILIGPDAVELPYREHYATTAERTENLVKKQQRFINGISASDVIT
                     YFAGTRAATYEEDFVIERSERVRNLVHAAGIQSPGLTSSPAIAHEVASMCIDVLKETH
                     EVEVRPNAKFNPRRNVPPDFARLPLEERQRIIKANPDYGRVVCRCEVITEGEIRAALR
                     SPVPPTTLDGLKRRVRTGMGRCQGGFCTPLVLDIACDETGIAPDKFTKKGGASYLISG
                     PTRPELEEAISVNPRRNAPIGHAEVLSATNDEDCTPQALGAYDVVIIGGGPAGLAAAI
                     AAHDAGAKVGLIEREARLGGILKQCVHDGFGTIEFNTHLTGPEYAERFMSQAAQRDIT
                     CYTQTFVLDVARNGDGRKLDNFRPSEGRNFALTVENRDGIFNIESKALVLATGCRERT
                     ARQVFIHGTKPAGIYSAGTAQYMVNVQGRLPGLRCVILGSGDVGLIMARRLTLEGATV
                     VAVYEAKSEPTGLPRNISQCLDDFGIPLYLSHTVTKVFGDERVEAIEIAQVDGEMKPI
                     VGTEQRIECDCVIVAVGLIPENELAETLGAEISNTTRGPKVDQTFQTTIPGLFACGNC
                     VHVHDLVDYVTQSGRVAGLSAAQYAQGERRIGRITHVCPSDDLLYSVPQFIDAEMANE
                     AFTIYFRS"
     gene            3618..>4758
                     /gene="glpK"
                     /locus_tag="PLM83_06780"
     CDS             3618..>4758
                     /gene="glpK"
                     /locus_tag="PLM83_06780"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOK71143.1"
                     /translation="MGEYILALDQGTTSSRAILFDHLGRSVGVASREFKQIYPKPGWV
                     EHDPNEIWRTQLKTARGVIDSTGVGPDEIAAIGITNQRETTILWDKSNGCPIGNAIVW
                     QCRRTAGCCEELKAAGEAKAIHEKTGLIVDAYFSATKIKWILDNAPDLRGRAERGEIL
                     FGTVDSWLIWNLTGGRVHATDYSNASRTMLFNIHTLDWDDEILEILDIPRAMLPEVRP
                     STGVFGYTQPSLLGAEIPIAGVAGDQQAALFGQACFSPGSAKNTYGTGCFMLMNTGED
                     VVMSRNNLLTTIAWGIDGRVEYALEGSVFIGGAVVQWLRDGLRIIDSAAESEECANRV
                     ADTGGVYVVPAFVGLGAPHWDPFARGTIVGITRGTERDHIVRAALE"
BASE COUNT          982 a         1193 c         1393 g         1190 t
ORIGIN      
        1 gatctgaagt agatcgtgaa ggcttcattt gccatttcgg cgtcgatgaa ttggggaacg
       61 gaatagagga gatcgtcaga ggggcataca tgggttatcc ggccaattct tcgctctcct
      121 tgggcgtatt gggcggcact cagcccggcc actctgccgc tttgtgttac gtaatcgacg
      181 aggtcgtgaa cgtgaacgca gttgccgcag gcaaagagtc ctggaatagt ggtttggaac
      241 gtctgatcaa ccttgggacc gcgagttgtg ttggaaattt cggctcccag cgtttcggcc
      301 agctcgtttt cgggaatcaa tccaactgcg acgatgacgc agtcgcattc gattctttgc
      361 tctgtcccga cgatcggctt catttcgccg tcaacttggg cgatctctat cgcctcaacg
      421 cgttcgtcgc cgaaaacctt tgttacggtg tgagagagat aaagagggat gccgaagtca
      481 tctaggcatt gggagatgtt ccggggaagg cccgtcggct ctgatttggc ttcataaaca
      541 gccaccaccg tggcaccctc cagtgtcagt ctgcgagcca ttatgaggcc cacatcgccg
      601 cttccaagta ttacgcacct gaggccgggc agccgccctt gcacgttcac catatactgg
      661 gctgtacctg cactgtatat gccggcaggt ttcgtcccgt ggatgaagac ttgtcgagct
      721 gtgcgttctc tgcaccctgt ggctaggacc aatgccttcg attcaatgtt gaatattccg
      781 tcccggtttt ccacagtaag tgcaaaattg cggccttccg agggcctgaa gttatctagt
      841 ttgcgtccat ccccattgcg ggctacatcg agcacgaacg tttgcgtgta acatgtaatg
      901 tcccgctggg ctgcctgcga catgaagcgt tcggcgtact ctgggccagt cagatgcgtg
      961 ttgaactcga tcgttccgaa gccgtcgtgg acgcattgct ttaggatgcc gccgaggcgt
     1021 gcttcgcgtt ctatgagtcc gactttggcg cctgcgtcat gtgcggcaat ggctgcagcg
     1081 agcccggccg gccctcctcc gatgatcact acgtcatacg ctcccaaagc ctgtggagta
     1141 cagtcttcat cgtttgtcgc agagagcact tccgcatgcc cgatcggtgc gtttctgcgg
     1201 ggattgacac tgattgcctc ctccagttcc gggcgtgtgg gaccggatat cagatacgag
     1261 gctccgccct ttttggtgaa cttatctggg gcgatccccg tttcatcgca ggcgatatcg
     1321 agtaccaggg gggtgcagaa tccgccctga caccgtccca tgccagtcct gaccctccgc
     1381 tttaacccgt caagggttgt ggggggaaca ggagagcgaa gcgcggcccg aatctcgcct
     1441 tccgtgataa cctcgcatct gcatacaacc ctgccgtaat ccgggtttgc cttgataatt
     1501 ctctggcgtt cttccagcgg gagtcgggcg aaatcagggg ggacgttcct gcgagggttg
     1561 aacttggcgt tgggcctgac ttcgacttca tgcgtctctt tgagcacatc gatgcacatc
     1621 gatgcaacct catgcgcgat ggcaggcgat gaggttaggc ccggcgactg tattcctgct
     1681 gcatgcacca ggttgcgcac gcgttctgac cgttcgatga cgaagtcttc ctcatatgtt
     1741 gctgccctcg tgccggcaaa ataggtgatc acatcagatg cggatattcc gttgatgaat
     1801 ctttgttgct ttttgacgag gttctcggtt cgttccgctg tagtcgcgta atgttcgcga
     1861 tagggcaatt cgacggcatc gggcccaatc aggatgttgc catcgatggt gggaatcacg
     1921 ccaccgccct tggtgtcagt gcgcgcctga tagacagggg gcatggagag ggtggatctt
     1981 gccaggttgc ctttcttctt gtcgaggatc agcatttcgc catgcctagg atggattgtg
     2041 aagaacatgt cgcctgcggc ctcggcaacc gtgtccgccc atagcccggc ggcgttcact
     2101 accgccttgg gaaagaggac accccgattg gtgacaactg ccagaatcct tccgtgttca
     2161 acgatgaatg attctgccat ggtgtttagc cagacggtag cgccgttttc aactgcattt
     2221 tcagcaaatg ctattgtgag catgaaaggg tctatgattc cgctttgcgg catgaagaag
     2281 gcgcctccga tgtcgtccct gacgttaggc tccatggatt gtagttcggc tgggggcaca
     2341 aatctgatct cacctgcgcg gtttcgtcgc gccctgttgg cgagaacggg ataagcgatt
     2401 ccatatattc gccgttctac gattatcgtc atgccgttgt atctgaaagg gacacctagt
     2461 tcatccgcga tggctgggat catcggattc cccaatgagt tgaattgcgc tttcttcgaa
     2521 ccgggggagg gcgccagggc cggatggatc atgccagcgt ttctgccgct ctgatgcatg
     2581 gcgaggtcgg cttctttttc tatgagacat gctcgaatat cccatctgga gagctctctt
     2641 aagattgcgg tgccgatcac tccgccgccg ataacgagaa cgtccatgcg ctctccttcc
     2701 agggacgcgt ctcgaatgga aggcgctttg gggccgggca tggccagaat tccatcgcaa
     2761 gtcaggcggt tgactacacc cttgtagcct agctttgctc cgcttttgcc ggcagcaaca
     2821 acatgatccc atgtgggcac atttccttcc aggactatgg cgccgtcttt ttcgtagcat
     2881 gagatgtcgc gattgagctt cttggcaagc gacttttcga gagaagacgc tttcgacatg
     2941 atactcacca ctcgataatt aatggggaga gcactgtttt gctaaaactc gggcataagc
     3001 ctttgcagac tgtgggcagg gcccaacaaa ttggtactaa tgatacggat gtacgtacct
     3061 taacactact acttatggac tcatgtgtca accctggact gaattggaat gcatggataa
     3121 atagtctcat gggagtgggc atcctgtttg ccagcttaaa gcggaatatc tattaaagct
     3181 agcaagttcc gccgatggcc aatggcggat ggggatgatg acgggattgg ccatgaaagt
     3241 gggctatgcc gatttgctcg gagcatcatc tgtaagatgc tgaatgaatc ggcttttgac
     3301 gcggttgggc ctcttgggga ttgtgaaggc gcgtggcatg atacgatatt ctgtttttgg
     3361 ggataacctg tcgtgtatag aggatttttg gccctttggg tagaaaagca ggtgggcatg
     3421 tgttattata ctgtcgtagt cggaaaaggg aaaagggatc atgttaaata ggacggagat
     3481 ttggagagtc ctacagcggg acggagctta catggcttct gctcgatgaa gggcttttct
     3541 ttttgctcgc ttaggccata ttcatagctc aatatgatgg tctaaacagt gggcctatcg
     3601 atgcggaggt gtatcgaatg ggggaataca ttctcgcgct tgaccagggg acgactagtt
     3661 cgcgtgcaat tcttttcgac cacttggggc ggagtgtcgg cgttgccagc agagaattca
     3721 agcagattta tcccaagcct ggctgggtgg aacacgaccc gaacgagatc tggagaactc
     3781 agctgaaaac tgcaaggggt gttatcgatt ccaccggtgt tggtcctgat gagatagcgg
     3841 ctatagggat aaccaatcag cgggagacga cgatattgtg ggacaagtcg aatggctgtc
     3901 ctatcggcaa cgcaatagtc tggcaatgca ggaggactgc gggctgctgc gaggagttga
     3961 aagctgctgg cgaagccaaa gcgattcatg agaagaccgg gcttatcgta gatgcttact
     4021 tttctgccac caagatcaag tggatcctcg acaatgcgcc cgaccttcga ggcagagcag
     4081 agcgagggga gatcctcttc gggactgtgg attcatggct catatggaat ctaacaggcg
     4141 gccgagtaca tgccacggat tattctaatg cgtcgcgcac tatgcttttc aacattcata
     4201 cactcgactg ggacgatgag attctggaaa tcctcgatat tcctcgggct atgcttccgg
     4261 aagtcaggcc ttctaccgga gtcttcggct atacccaacc tagtctgctt ggggcggaga
     4321 ttcctattgc tggcgtagct ggcgaccagc aagctgccct tttcgggcag gcatgttttt
     4381 cgccgggcag tgcgaaaaac acgtacggga caggttgctt catgctgatg aacacgggcg
     4441 aggatgtagt catgtccagg aacaatctcc tcactactat tgcgtggggc atcgatggca
     4501 gggttgagta tgcacttgag ggcagcgtct ttattggcgg cgcggtggtc caatggctca
     4561 gggacgggtt gcgaataatc gattctgcag ccgaatccga ggaatgcgcc aatcgcgtgg
     4621 ccgatactgg tggcgtgtat gtggtccctg cgttcgtagg acttggggct cctcactggg
     4681 atccgttcgc acgcggaacg atagtgggga ttacccgagg cacagagcgc gatcacatag
     4741 tcagggcggc tctggagg
//
LOCUS       DAOSAI010000177         4736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11377_length_4736_cov_3.566332, whole genome shotgun sequence.
ACCESSION   DAOSAI010000177 DAOSAI010000000
VERSION     DAOSAI010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11377_length_4736_cov_3.566332"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /gene="holA"
                     /locus_tag="PLM83_06785"
     CDS             <1..677
                     /gene="holA"
                     /locus_tag="PLM83_06785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOK71144.1"
                     /translation="TSLPRKAVAAAGGNLVAVEFRPAYLKDARSYAYSRAKELGVEIE
                     PEAVELLLEFVGADYGAITQEMDKLITYLGPDKSVGRMRITVDDIRTAVGRSPTQTVF
                     EFCDAVIGGDCSGAMRALDDLMSTERHPLSILNTLATQVRRVMSARCELDRGLTGDEA
                     ARAISRSPGKADWAARKAVGQAAKLSQADIARMISRIARADLDFKTGTMPDRLLMEVL
                     IAEICA"
     gene            complement(773..1057)
                     /gene="rpsT"
                     /locus_tag="PLM83_06790"
     CDS             complement(773..1057)
                     /gene="rpsT"
                     /locus_tag="PLM83_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOK71145.1"
                     /translation="MPNTRSAKKRVRQARTRTLRNTSVKSSVKTAVRKCHEAMDELGP
                     EACAKELNHAYSTIDRAAKKGVIHPNKAARLKSSLATKAVNMGKAPEAGR"
     gene            1651..2736
                     /gene="gpr"
                     /locus_tag="PLM83_06795"
     CDS             1651..2736
                     /gene="gpr"
                     /locus_tag="PLM83_06795"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HOK71146.1"
                     /translation="MGFDRNFGAKWRPSKKLQHLSECDTGLSGGGYLTDLAVEANELV
                     QRASRAQVPGVSSQVEEMDFGSITRVRVQTEQAARSLGKAVGNYVTIDAPELPLRDKG
                     LQEDVSEALARELVSLIRANFNNVDFWTIPDFTTLVCGLGNWNATPDAVGPMVAGEVL
                     VTRHVYSLTPPEKRGGLKSVAALSPGVLGLTGVETAEIIAGVVARVRPHLIIVVDALA
                     ARSVSRLGTTIQISDAGIQPGSGVGNRRFGINRESMGIPVIAVGVPTVVHALTIISDA
                     LEIVGSSQQPGVGRSAAGPLSELGVGQPGLPPAVAQMLQPYFGTLIVTPKEVDVLVKE
                     LSHVVAGGINYALHPAIDEDEIYLYLQ"
     gene            2794..4293
                     /locus_tag="PLM83_06800"
     CDS             2794..4293
                     /locus_tag="PLM83_06800"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HOK71147.1"
                     /translation="MQRGRGTRRKSRRAGGLKIGPLGGLFVFYVILCFAAVGLIIGTR
                     AVKDGVSVPAVSGVPGQDVVGDGETLASEPSLWSKVSKMVSSFLSLFRPSSDRSLPNR
                     SAEERFGSIGPRLVNAALRAMGGLDFADPRTLIRAELPSILAAQRTSDNPSRSATYGR
                     GSGEDPDISAPMRAEGSLVSNSTLVSGQLSDAELESLTSSGLLVFGDQPSSSDPEAGT
                     EVPDGTSVQVIVSSESANAAVPAVSKPVVESSSNTSSPGRDADSQGANSVPTREPVPS
                     QPSKFPQLAAREIADNMVLRGASPKIAILHTHTSEAYRATSGRDYCWGKPDGVVKVGA
                     AMAEELSEVLGVPSIHASTVHDWPDWTLSYSKSLKTMTSLVKSYSSLRAVIDIHRDSL
                     PANREKLKTAMVGGEKVARVLFVVTDDSSGLAHPNWRKNYHFALELSAEMDKMYPGVS
                     RGVAIVKNARFNQHVHPGAIILEIGGTDNTVEEACRSARLVARALARII"
     gene            4297..4551
                     /locus_tag="PLM83_06805"
     CDS             4297..4551
                     /locus_tag="PLM83_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71148.1"
                     /translation="MRRVDQWETGRGRRAFAVVPAIACLFLIVVFIAIAVAGSNSSQL
                     AGAGSQWSPVRIRAVGGGYHGIVLEAFGRAVRIEFENALK"
BASE COUNT         1008 a         1159 c         1493 g         1076 t
ORIGIN      
        1 ccaccagctt gccgcggaag gcggtagctg cagctggagg caatcttgtt gcagtggagt
       61 ttaggccagc ttatctgaag gatgccagat cgtatgcgta ttctcgtgcg aaggagcttg
      121 gcgtggaaat cgagcctgaa gctgtggagc tccttcttga gtttgtgggc gctgactacg
      181 gcgccatcac gcaggagatg gataagctca tcacatacct agggccggat aaaagcgtgg
      241 gaagaatgcg cattaccgtg gatgacataa ggactgccgt gggaagaagt cctacgcaga
      301 cggttttcga gttctgtgat gcggtgatcg gcggagactg ttcgggggcg atgcgcgctc
      361 tggatgatct gatgagcacg gagaggcatc ctctgagcat attgaatact ctggccactc
      421 aggttcggcg tgtgatgtcc gccagatgcg agctcgaccg tggtttgacc ggtgacgagg
      481 cggcgcgggc gatttcgaga agccccggaa aagctgattg ggcagcgcgc aaggccgttg
      541 gacaagcagc aaagctctca caggcagaca tcgccaggat gatatcgcgc atcgcccgag
      601 cagatctcga tttcaagacc gggactatgc ctgacaggct gctcatggaa gtgttgatcg
      661 cagaaatctg cgcatagcaa aaactccagc aacgattgtt actggagctg caaactgaat
      721 agccggacga cgcgggtggc gcaggttctg cgctatcgtt ttgtatgcga aactacctgc
      781 cggcttcagg cgcttttccc atgttgactg cctttgtggc aaggcttgat ttcagacgcg
      841 ctgccttgtt agggtggatt acgcccttct tggcggcgcg gtcaatcgtg ctgtacgcgt
      901 gattaagctc cttggcacag gcctcaggtc ccaattcgtc catggcttcg tggcacttgc
      961 gaacagctgt cttgacggag gacttgaccg acgtattcct cagcgttctc gtcctagcct
     1021 gtctaacccg cttctttgca gatctggtgt ttggcactct cagtcacctc cccctccgcc
     1081 atctgatcgc acgcctatga ttctaccacg ggcgttctgt cattgcaagt aaatccagat
     1141 aagaggggaa taagggcaag ttgtgatgga atcggctgtt gagaatggca ttcttgcgga
     1201 gacgtgaaat atgctgttct ctggcttcaa gcgagtccgc ccgtggaaga ccaattccag
     1261 agcgtttctg gaggttagta tatggtggct gatttaagat gagtccttcg ttgataagga
     1321 gccgtcgttc caaatggagc tttcatccga aatgtgttgt cttggcggga agaattgata
     1381 tttgcgacgt ctgacaactc gacagcgagg gtaccctgtg gccatgttag cacctcaata
     1441 ctgaaatcga cgaattgagc cggacgtcgt ttccggaggc cttgattata ggctgagtcc
     1501 ggcaatggca ctccagcccg gtcgggagag gtttcggtcg ttgaaggatc ttggggaaga
     1561 tgccagcaga cattgatgaa cgggcctccg agtgggaaag actaggcatg tgcagataca
     1621 gactgcgcat cccgaggagg tgcattagga ttggggtttg accgaaattt cggcgcaaaa
     1681 tggcgtccgt ccaaaaagct ccaacatctt tcagaatgcg acacggggct gagcggtggc
     1741 ggctacttga cggacctggc tgttgaggcc aacgaactag tacaaagggc gagccgggcc
     1801 caggtgcccg gagtcagttc ccaagttgag gagatggact ttgggtcaat aacgcgagtg
     1861 agagtccaga cggagcaagc tgcgcggtct ctcggaaagg cagtggggaa ttacgtgact
     1921 attgacgccc ctgagctccc cctgcgtgac aagggattgc aggaggacgt gtcggaggcg
     1981 ctcgcccggg agttggtctc cttgatcagg gccaatttta acaatgtgga tttttggacg
     2041 atccccgatt tcacgactct cgtatgcggg ttaggcaatt ggaacgcgac ccctgatgct
     2101 gttggcccga tggtggcagg cgaggtttta gtcactcgcc acgtatattc ccttactccg
     2161 ccggaaaaac gaggggggct caagtcagtg gctgcgttat ccccgggagt tctgggcttg
     2221 accggagtcg agacggccga gataatcgcg ggcgttgtgg cacgggttag accacacctg
     2281 atcattgtcg tggatgccct ggccgcgcgc agcgtctcta gacttggaac tacgattcag
     2341 atttccgacg ccggcatcca gccgggatct ggtgtaggca ataggaggtt tgggattaac
     2401 cgtgagagca tggggattcc tgtaattgct gtgggggttc caactgtggt gcacgcgctc
     2461 acgataatct cggatgccct cgaaatcgtc ggtagctcgc aacagcccgg cgtcgggagg
     2521 tctgcggcag gtcctctgtc agaattgggg gtggggcagc cgggtctgcc tcctgcggtg
     2581 gcgcagatgc tccaaccata tttcgggacg ctcatagtta ctcccaagga ggtggacgtg
     2641 ctcgtcaagg aactctctca cgtggtggct ggagggatta actacgcgct ccatccggcg
     2701 atagatgagg acgagatata cctatacctg caatgagggg gtatgaatac ctgatgggcc
     2761 aggcgcatac agctgcataa agaggtgatc ccattgcagc gagggcgcgg aacgagacgg
     2821 aaatcaagga gggccggcgg tctcaagatt gggccgttgg gcggcctatt cgtattctac
     2881 gtgatcctct gctttgcggc tgttggcctc ataataggca cgcgtgcagt caaggatggc
     2941 gtttctgtgc cagctgtctc gggcgtgccg gggcaggatg ttgtcgggga tggcgaaacg
     3001 ctcgcctctg aaccctcttt gtggagcaag gtttcgaaga tggtgtcgag cttcttgagc
     3061 ctgtttcggc caagtagcga ccggagtctt cccaatcgat ccgctgagga aaggttcggc
     3121 tcgatcggcc cgaggttggt gaatgcggca cttagagcga tgggagggct ggactttgcg
     3181 gacccacgta ctctaatccg agccgaactc ccttccatcc ttgccgcaca gcggacctcg
     3241 gacaacccgt cgcgctccgc aacatatgga aggggttccg gtgaggatcc ggacatttct
     3301 gcgccgatgc gcgccgaagg ctctctcgtg tccaattcga ccctggtgtc gggccagttg
     3361 tctgacgcag agttggagtc gttgaccagc tctgggctct tggtcttcgg agatcagccg
     3421 tcctcatccg acccggaggc aggcacagaa gtcccagatg gcacgtccgt tcaagtcata
     3481 gtttcatcag agtctgcaaa tgcagctgtg cccgcagttt ccaaacctgt tgtggaaagt
     3541 tccagcaata catcgtctcc tgggcgagac gcggattcac agggtgcgaa tagtgttccg
     3601 acgagggaac cggtgccgag tcagccgtcc aagtttccgc agttggctgc gagggagatt
     3661 gccgacaata tggtgctgcg cggggcatct cctaagatag ccatacttca tacgcacaca
     3721 tcagaagcct atcgggcgac gtcaggaagg gattactgtt ggggaaaacc ggatggggta
     3781 gtgaaagtag gcgcggcaat ggccgaagag ctgtcggagg ttctgggggt tccgtcaatt
     3841 cacgcgtcaa cagtacacga ctggccggac tggactttga gttactcgaa gtcactgaag
     3901 actatgacgt ctttggtgaa gtcgtattct agcctcagag cggtcataga tatccatcgt
     3961 gattcattgc ccgctaaccg ggaaaaactg aaaacggcga tggtcggcgg tgaaaaggtg
     4021 gcgcgtgttc tgtttgtcgt gactgacgac agctcaggac ttgcacatcc taattggagg
     4081 aagaactacc acttcgcgtt ggagctttct gccgagatgg ataagatgta tcctggggtt
     4141 tccaggggag ttgctatcgt gaagaatgcc cgattcaacc agcacgtcca tccgggcgcc
     4201 atcatcttgg aaattggagg gacggataac actgtagagg aggcttgcag atcggccagg
     4261 ctcgttgcgc gggctctggc caggatcatc tagccaatga ggagagtcga ccagtgggaa
     4321 acggggagag gccgccgggc gtttgcggtg gtccctgcca ttgcatgcct tttcttgata
     4381 gttgtgttca tagctatcgc agtggcgggc agcaattcgt cgcagctggc gggagccggt
     4441 agccagtgga gccccgtccg gattcgggcg gttggcggag gttaccatgg gatagtgctc
     4501 gaagctttcg gacgagccgt aaggatcgaa tttgagaacg cactgaagta gtatgagagg
     4561 tccgggcaat cggtgtcgag catgtctccg aagagtcatt gctacatgtg ttataattta
     4621 gtgtggagtt cctaccaggc cgcgaaaagc ggcgcatagt ggctctgagg tggatatgaa
     4681 catagaaggt atcagaaact tttgcattat tgcccacatt gaccatggga aatcta
//
LOCUS       DAOSAI010000178         4731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11393_length_4731_cov_4.578058, whole genome shotgun sequence.
ACCESSION   DAOSAI010000178 DAOSAI010000000
VERSION     DAOSAI010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11393_length_4731_cov_4.578058"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLM83_06810"
     CDS             <1..710
                     /locus_tag="PLM83_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOK71149.1"
                     /translation="LAQSVGGLGEGPPIVGDLVSCGDMVVLVIPADAQAPKGRLILPQ
                     VQTIRDVLDHEGIAVAVRIDQLRQLFADKDFLPRLVVTDSQAFGEVAPVVPESVPMTS
                     FSILFARHKGDFELLVEGARAIEGLEPGDVVLISEACTHHPIEDDIGTVKIPRLLNAK
                     VGGELRYEWQRGGDFPEDLDRFKLVINCGGCMLNRREMCSRLEAVADAAVPVTNYGMA
                     IAACLGILPRALRPFAL"
     gene            1152..1574
                     /locus_tag="PLM83_06815"
     CDS             1152..1574
                     /locus_tag="PLM83_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71150.1"
                     /translation="MAISPRLLEIRSCLVHDYEPLIDYGAWRVAAICSSEHNAVDGVK
                     SLERHNETDEVFLLLAGRAFLIIGEGDASVETFHAVAMEPLKAYNVKRGVWHSCVLDT
                     DSVVLVVENRDTTHLNSEFLDLPSEICHQLPDICGEIV"
     gene            1997..2887
                     /locus_tag="PLM83_06820"
     CDS             1997..2887
                     /locus_tag="PLM83_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71151.1"
                     /translation="MSRRRIAIVLVCFFILSLGLVVGSPAYCGETLASTTYYQGGKRF
                     QVDVDLGANWEMTLTVYRIELSGKPRKLWSYTGDHIELEMVRDVDGDGFVEVLAMEYG
                     GNTYACPILFRVDKNEKVRQIPIDLGKMYEKPNYMLIARAASFIDLDDDGVDELIAWV
                     PQYWMPYYANSDMVYASIICKAKDNRYVAAAGDYASVYRFLISELRNEIVTYGTDILE
                     LDVGPYLQNCCLLLLYRSLVGEMTQGLQEFDTLFPHALKVIDPVADKWFADTWKDFMK
                     RRVDLLTQATRDFGGIAQKR"
     gene            3091..3894
                     /locus_tag="PLM83_06825"
     CDS             3091..3894
                     /locus_tag="PLM83_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71152.1"
                     /translation="MERSRIYGAFERVSRKWWFYVLVVMALGIPPYASTGVAPEQIAS
                     LVQEVLSQGLTAYSALMPMLHIAVAALVVLVGIYGNRVGRLFSAFMGLHYIFIAIAQN
                     TAVTPTFGYAVISSNVLLFMVIGIMWLWDAAIGKTDYTLRGPHPWWTYWVLPLAVLAF
                     WGPDVPWSFHLRYLVTSSSVGAFCLMTPIYLSVLSFCPRYNLPAVRLTSFVGILMAIF
                     NFMIGLSLCIGGNASLGIYQVFIHLPLAALSTYSFISSFRAGRARQPCS"
     gene            3973..4461
                     /locus_tag="PLM83_06830"
     CDS             3973..4461
                     /locus_tag="PLM83_06830"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOK71153.1"
                     /translation="MEPFASFDHERYMREALSEAEKAFERGDRPIGAVIVHDGEIIAR
                     GSNRFFSESSYIAHAELNALVSAAPYLYDHGRECVIYTTVEPCVMCLGAIIHANIRNI
                     AFGMHDNYIQARKIIDAVEYVDRRVHNYLGGILEKECVELYRRYSEREAQLMLTGTQN
                     SW"
BASE COUNT         1112 a         1100 c         1346 g         1173 t
ORIGIN      
        1 ggttggctca atccgtaggc ggactgggcg aggggccccc catagtaggc gatcttgtaa
       61 gctgtggaga catggtggtt ctggtcattc cggcagatgc ccaagccccg aagggcaggt
      121 tgattctgcc tcaggtccaa accataagag atgttttaga tcacgagggg attgcggtcg
      181 ccgtaagaat tgaccaattg aggcagctct tcgccgataa ggatttcctg ccgagattgg
      241 ttgtgactga ttcccaagcc ttcggggagg tagcccccgt agtcccggaa tccgttccca
      301 tgacatcttt ttcgattctc tttgcacgcc ataagggtga cttcgaactt ctggtcgaag
      361 gcgctcgcgc catagaaggc cttgagcctg gcgatgtagt gctcatatcc gaggcgtgta
      421 ctcatcatcc catcgaagac gacataggga ctgtgaagat cccccgcctt ctcaatgcga
      481 aagtcggcgg agagctgaga tatgaatggc aacgcggagg cgattttccg gaggatttgg
      541 acagattcaa gcttgtcatt aactgtggcg ggtgtatgct taacaggaga gagatgtgtt
      601 cgcgccttga ggccgtggca gacgccgctg ttccagtcac aaactatgga atggcaatcg
      661 cagcgtgtct ggggatcttg cccagggcct tgcggccttt cgcactctga aatgaaccgt
      721 ctgcataagc ccggtgatcc gcgagtacct cgaggcctaa ggagcagaga tagcggactg
      781 tgcggccgat ccccaagtcg aatctttatt gcccgatact tgaattaggt gtgtcgacgc
      841 cccaactgca ttggtcataa tgcttcagcc ttgcccacac catgcccaaa aaggtgagac
      901 aacaaggcga gggacgtcga cacttcacat tatctaaggg acttttctgc agacgggagg
      961 tgtatttggc cgacaaggtc ggctccaaga cgcaaggctc cggcttggtc ctgcgatacc
     1021 aggccggagc tttacatcgg aagaagagag ttgaacctcc cgccaactac attctcttcc
     1081 ttgataggct gaccgttgta ggaatataat agttatactt agccgtgaac aaccatccgg
     1141 aggtgggcgt tttggcgatt agtccaagac tgcttgagat acggagttgc cttgtgcatg
     1201 actacgaacc gttgatagat tacggtgcat ggcgtgtggc cgctatatgc tcctccgagc
     1261 acaacgcagt tgacggggtg aagtcccttg aaaggcataa cgagacagac gaagtctttt
     1321 tgctcttggc aggcagggcc tttctcatta taggcgaggg agacgcaagt gtcgaaacgt
     1381 ttcatgctgt ggcgatggag ccgctcaagg catacaacgt gaagcgaggc gtctggcatt
     1441 cgtgtgtgct cgacacagat tctgtagtgc ttgttgttga gaatcgcgat actacccacc
     1501 tgaactcgga gtttctcgat cttccgtccg aaatatgcca tcaattgccc gatatctgcg
     1561 gtgagatagt ttagagcctc ggcatgttcc gcgtcaggct gccgctttgc cagttatgca
     1621 aatctcaagc cacatcggaa aaacctgagc cggtgaacgc caataacctc aaaagacaga
     1681 ggagtttcgg acatctagtg cgaaacttac atgcagaagc atatcctcat cctgcctgtg
     1741 ctggtagctc gcgacctatt cccaaactaa gtcatgcggg cgactccgag ccgattctta
     1801 tcatgatcaa tctcttggtt tttatagatc gagcgtgatt acaaaatgaa ttcaagcgtc
     1861 gagtcacacc cttacacctc cttctttgct gattctcggg agggccgtta agttagctga
     1921 aaagaaaatg gtgacttgga aatgatgtat cattttgtgc caatgggctg taattgtcga
     1981 gatgaggtga tagtcaatgt ccagaagaag aatagcaatc gtccttgtat gttttttcat
     2041 tctgtcgctc gggcttgtag tgggaagccc ggcatactgc ggcgaaacct tggcaagcac
     2101 cacatattat cagggcggca agagatttca agtcgatgtt gacctggggg caaattggga
     2161 gatgaccctc accgtctacc ggattgagct gagcggaaaa ccgcgcaagt tgtggagtta
     2221 tacgggtgac cacatagaat tggaaatggt gcgggatgtt gacggggatg ggttcgttga
     2281 agtgttggcg atggagtacg gcgggaatac ctacgcctgc ccgatcctgt tccgggtgga
     2341 taagaacgag aaggtgcggc agataccgat cgatctaggg aagatgtatg agaaaccgaa
     2401 ttacatgttg attgccagag cagcctcgtt catagatctg gatgatgatg gagtggatga
     2461 gcttatcgcc tgggttccac agtattggat gccgtattac gccaatagcg atatggttta
     2521 tgcgtcgatc atatgcaaag cgaaggacaa ccgttatgtg gcggctgccg gcgattatgc
     2581 ttcagtatac cggttcctca taagcgaatt gagaaatgaa atagttacat acggaactga
     2641 catcttggaa ctagacgtcg ggccatacct ccaaaattgc tgtttgctgt tgctctatcg
     2701 atcccttgtt ggcgagatga cgcagggact ccaggaattc gataccctgt ttccacatgc
     2761 attgaaggtc atcgaccctg ttgctgacaa gtggtttgcc gatacatgga aggacttcat
     2821 gaagcgcaga gtggatctgc tgacgcaggc aaccagggat ttcgggggga ttgcgcagaa
     2881 acgctgaatc agtgcgggcc gattggagca tgaacgagca gccccactac tgggattgca
     2941 tggtaatggc gccaaccgac acctgacgaa tggccaacta ttcattgaag gtatattgct
     3001 gacgaagatg gaagtatcac acgcgtgtct gttgcggaag gactaatgcc taaagcccag
     3061 gttgtgtgac cggggaggtg ctcaaagcga atggagcgga gcaggatata tggcgctttc
     3121 gagcgcgtga gtagaaaatg gtggttctac gtcttggtcg ttatggccct ggggattccg
     3181 ccttatgcat ccacgggagt agcccctgaa cagatagcca gtttagttca agaagttctt
     3241 tctcaggggt tgacggcata tagcgctctc atgcccatgc tgcacattgc tgttgcagct
     3301 ctggtcgtgt tggtgggcat ttacggaaac agggtgggaa gactcttcag cgcttttatg
     3361 gggcttcact atatattcat agccattgcg cagaacactg ccgtaactcc tactttcgga
     3421 tatgccgtta tcagcagcaa tgtccttttg ttcatggtaa tcgggataat gtggttgtgg
     3481 gatgcagcga tcgggaagac cgactacacg ctgcgagggc cgcatccgtg gtggacgtat
     3541 tgggttctgc ctctggcagt cttggcgttt tggggacctg atgtcccgtg gagtttccat
     3601 cttcggtacc tagtgacgtc cagttctgtc ggagcgttct gcctgatgac gcctatctac
     3661 ctttctgtct tgagcttctg tcctaggtat aatcttcctg cggttcgact tacaagcttt
     3721 gtcggaatac tcatggccat attcaatttc atgataggct tgtctctttg cataggcggc
     3781 aatgcaagcc tcgggatata ccaggtattc atacatctgc cgctggcggc actgtcgaca
     3841 tattctttca taagctcgtt tcgggcgggt cgcgcgaggc aaccctgttc gtagcgagga
     3901 tccaattgat cttcagaatg agcgtggggg agccctcgag ttaattcgtt aagaggtgga
     3961 gggaattacg aagtggagcc ttttgcgtct tttgaccatg agaggtacat gcgggaggcc
     4021 ctatctgagg cagaaaaggc ctttgagcgg ggcgacagac cgataggcgc cgttattgtg
     4081 catgacggtg agataatcgc cagaggttct aataggttct tcagcgagag cagctatatt
     4141 gcccatgccg agcttaacgc attagtatca gcagcaccct acctgtatga ccatggacga
     4201 gagtgcgtca tttacaccac tgttgagcca tgcgtaatgt gtcttggcgc tattatacat
     4261 gccaacatca ggaatattgc gttcgggatg cacgacaact acatccaagc ccgtaagatc
     4321 atcgatgcgg ttgaatacgt agacaggaga gtccataatt acctcggcgg catactcgaa
     4381 aaggaatgtg tggagcttta caggcgctac tccgaaaggg aagcacagct aatgctgacg
     4441 ggaacgcaga actcctggta ggtttgaaaa gcgctcaatg gatattgaca ggtgagcgcc
     4501 aagggggcaa agactccaga tagcgtgaat cgcgaaaacg tgcaggaatc tgcatcatcg
     4561 gcagcctggg ttttgagtag actccgatcg cgcgagacgg aggccgtttc tggcagccgg
     4621 ttactgcgga ggctcgcccc ttggaggttc gtcctgcaca cctgctaagc gacttcaaat
     4681 tggttcttgc gagagtctta tgcgtaagac catgcggcaa ggctgtttta c
//
LOCUS       DAOSAI010000179         4730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11396_length_4730_cov_4.236578, whole genome shotgun sequence.
ACCESSION   DAOSAI010000179 DAOSAI010000000
VERSION     DAOSAI010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11396_length_4730_cov_4.236578"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..567)
                     /locus_tag="PLM83_06835"
     CDS             complement(10..567)
                     /locus_tag="PLM83_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HOK71154.1"
                     /translation="MRPTGIVRRIDELGRIVVPKELRRSLRIHEGDSVEIYVDPKGSV
                     VLKKYSPVGQLKVVATDMAESLSASSGSVALICDRDVVIAASGPLEEELIDRRVGRAV
                     EKAMAEKQALMVHFSGGGDASSILANDGPDSVAMISAAIAPIIADGDPVGAVVIGTTA
                     SDRTVGDLEESLAITAAGYLARQVE"
     gene            complement(604..4221)
                     /gene="mfd"
                     /locus_tag="PLM83_06840"
     CDS             complement(604..4221)
                     /gene="mfd"
                     /locus_tag="PLM83_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOK71155.1"
                     /translation="MTGIVDLLLDSTAFTEALRRVERGSRATHIVGLSGSQKSLFISG
                     LFCHARRTVFVVTATQHDAESLSGDIATMIGSDRVFVFPAVDVMPYEETPMSADLSAA
                     RAKAMAALARGEKCVVLAPARALGRRMVPVEIFRGAMFDIRVGQRCDLDGLVYMLAAS
                     GYEILGKVDGRGQASVRGGIVDVFPNDADRPVRIEFFGDEIESIRVFDPDTQLSQEML
                     GEATITPAREFMYDRERLERARMSIREELDRVGAGAAGKGRSVSADSAQAPSMLSPSS
                     MVMARTRNQLRERVMHHLERFETGAYFDNDEQYLPFFYSERVMVADWAEDALFVLDEP
                     VRLKEALDGYESDIRETYATLLEADMVLPSQADSYMNAAEIIDAIKRRQIVSMSLLAR
                     APEGSCGAGPGGALAGYGDHIIAQTKQAEIFAGRIDDFIHEIKNFRKRGYRVVCMAST
                     EERCNRLAELLEGKGISPICASTLDVVPEAGTASVVRGELSSGFKCPDIKLLVLTEAE
                     IYGRQKKRRRFADVEEGARIASYTDLSPGDYVVHVNHGVGKYGGVQTLEAAGVQRDYL
                     LVQYAGEDKVYVPTDQIALLQKYIGMEDSPPRLNRLGGTEWTRTKAKARESVQDMAED
                     LLRLYAARESVKGYAFSPDTVWQQEFEDLFPYEETPDQWRAIVDVKRDREKPIPMDRL
                     LCGDVGFGKTEVALRAAFKAAVDGKQVAVLVPTTILAQQHYNTFSERFHGFPMKVAIL
                     SRFQSEAEQKQVIRGLKMGSVDVVIGTHRLLSQDVKFRDLGLVVVDEEQRFGVAQKER
                     LKELRKEVDVLTLTATPIPRTLHMAMTGVRDMSLMETPPENRFPIRTYVVEKNDSLVR
                     EVILREIDRNGQVYFVHNRVHDIEEVAGRLRRLVPEASIGVAHGQMPEERLERVMLQF
                     MEREFDILVCTVIIESGIDIPNVNTIIIDDADNFGLAQLYQLRGRVGRTNRVAYAYLL
                     FNRDAALSEIAEKRLAAIREFTSLGSGYKIAMRDLEIRGAGNILGPEQHGHIAAVGFE
                     MYCRLLEETIKELRGEKKEPEVPPTVIDLPVDAYVPDSYVPDSRQKIEVYKKINGIES
                     LEDASDISKEFIDRFGPLPPPVENLIAIAALKGLARDLGAVNVSSERQSVVVGFADGV
                     SYSASDLMRIARPFRGRVALAVGRTQRLRVKTHGMSEREILHTLIGILSEMKQGVAVN
                     Q"
     gene            complement(4273..>4730)
                     /gene="pth"
                     /locus_tag="PLM83_06845"
     CDS             complement(4273..>4730)
                     /gene="pth"
                     /locus_tag="PLM83_06845"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOK71156.1"
                     /translation="KFRRASHCLSLVAKGTIVGADTLIVKPQTYMNNSGIAVARATRQ
                     YGAGPESLIVVYDDMDLEVGRIRIRAKGSAGGHKGMGSIIEHMGTENIARVRVGIGRP
                     PPWEDSVHHVLDTFARDELPIISDAVINAVSAILCIVEDGIETAQTRFN"
BASE COUNT         1048 a         1524 c         1079 g         1079 t
ORIGIN      
        1 gctgttcgtc tactccacct gccttgccaa ataccccgca gccgtgatag ccagactttc
       61 ctcaagatcg cccacagtgc ggtcactagc ggtagttccg atcactactg cgcctacagg
      121 gtctccatct gcaatgatag gggctattgc agcggaaatc atggctacgg aatccggccc
      181 atcattcgcc agtatgctgg acgcatcccc gcctccgcta aaatggacca tcaatgcctg
      241 cttttcggcc atggccttct ctaccgcacg gcctacacga cgatcgataa gctcctcttc
      301 gagcggaccc gatgcagcaa tcacaacatc cctatcgcat ataagcgcta cactgcctga
      361 tgacgccgaa agcgattcgg ccatgtcagt tgctacgacc ttgagctgcc ccacaggcga
      421 gtatttcttc agcaccacgc ttcccttggg atccacatat atctcaaccg aatcgccctc
      481 atgtattcga aggctccttc tgagctcctt gggcaccaca atcctgccca actcatcgat
      541 gcgcctcaca attccagtag gtctcatcca caataccctc ctgcatatca ctacgtaatt
      601 tggtcattga ttcactgcta cgccctgctt catctcggac agaatgccta tcagcgtatg
      661 caagatttct cgttccgaca tcccgtgcgt cttgaccctg agtctttgcg ttctccccac
      721 cgcaagtgcc actcttcccc taaacggcct tgcgatcctc atcaggtcgc ttgctgagta
      781 gctcacccca tcagcaaacc ccacaaccac actctgcctt tcactggaaa cattcacagc
      841 ccccagatcc ctcgccaaac ctttcaacgc agcaatggca atgaggtttt ccacgggagg
      901 cggcaatggt ccaaatctat cgatgaattc cttcgaaatg tcagaagcat cttctaacga
      961 ttcgatgccg ttgatcttct tgtacacttc tatcttctga cgcgaatcgg ggacatagct
     1021 atctggaaca tacgcgtcaa caggaagatc gatcacggtg ggaggaacct cgggctcctt
     1081 cttctcgccc ctcaattcct ttattgtttc ctccaagagc ctgcaataca tctcaaatcc
     1141 gaccgcggca atatgtccgt gctgttccgg cccgagaata ttacctgctc cccgtatctc
     1201 caaatcccgc atggctatct tgtagcccga ccccaagctc gtaaattcgc gaatcgcagc
     1261 aagccttttc tctgcgatct cgctcagagc cgcatcgcgg ttgaacagaa ggtaggcata
     1321 ggccaccctg ttcgtcctcc cgacccttcc acgcaactgg tagagctggg caagcccgaa
     1381 gttatccgca tcatcgatga ttatcgtgtt gacgttggga atatcaatgc cagattctat
     1441 tataaccgta caaacaagaa tatcaaactc gcgttccata aattgtaaca ttaccctttc
     1501 aagcctctcc tccggcattt gtccgtgggc aacgccgatg gatgcttcgg gtacaagccg
     1561 cctcagcctt ccggccactt cttctatgtc gtgcacccta ttgtggacga aatagacctg
     1621 cccgtttcgg tcgatctccc tcaaaatgac ctccctcaca aggctgtcat ttttctccac
     1681 tacgtaagtc cttatgggga acctgttttc gggcggagtc tccatcaggc tcatgtctcg
     1741 aacccctgtc atcgccatat ggagagtcct gggaatgggc gttgccgtga gcgtgagaac
     1801 atccacttcc ttgcggagct ccttcagacg ttccttttgg gccacgccaa acctctgttc
     1861 ctcgtcgacc accacgaggc cgagatcgcg gaacttcacg tcctgcgaaa gcaggcgatg
     1921 ggttccgata acaacatcaa cagatcccat ctttagacct cgaataacct gtttttgttc
     1981 cgcttccgat tgaaacctcg ataggattgc caccttcatg gggaacccgt ggaatctctc
     2041 ggaaaacgta ttatagtgct gctgcgcaag gatcgttgta ggcacgagta ccgcgacctg
     2101 tttgccgtct accgcggcct tgaacgcagc cctcagcgcc acctcggtct tgccaaatcc
     2161 cacatccccg cagagcagtc tgtccattgg tatgggcttt tccctgtccc ttttcacatc
     2221 aactattgcg cgccactggt caggagtctc ctcgtaaggg aaaagatcct caaactcctg
     2281 ctgccatacg gtgtctggcg aaaatgcgta gcccttcacg gattccctgg ctgcgtaaag
     2341 tcgaagaagg tcctcggcca tgtcctggac tgactcccgc gccttcgcct tggtgcgcgt
     2401 ccattccgtc cctccaagtc tattcaggcg ggggggcgaa tcttccattc ctatgtattt
     2461 ctgcagaagg gcaatctgat cagtggggac atacaccttg tcttccccgg catactggac
     2521 cagcagataa tcgcgttgga cccctgccgc ctccaacgtc tgaaccccac catacttccc
     2581 cactccgtgg ttgacatgga ctacgtagtc cccgggcgac agatccgtgt aactggcgat
     2641 ccgcgcgcct tcctcgacat cggcgaaccg ccgcctcttc ttctgccggc catatatctc
     2701 ggcttctgta agaaccaaaa gcttgatatc gggacactta aaccccgaag acaactcgcc
     2761 ccggactaca cttgccgtgc ccgcctccgg gacaacatca agggtcgaag cacaaatcgg
     2821 cgagattcct ttgccttcga gaagttcagc cagcctgttg catctctctt ccgtcgaggc
     2881 catacagact accctgtatc ctcgtttccg gaagttcttg atctcgtgaa taaagtcgtc
     2941 gatgcggcct gcaaatatct ccgcctgttt cgtctgggca attatatgat ccccgtaacc
     3001 tgccaaagcg ccgccagggc cggctccaca gctgccctcc ggcgccctag cgagcaaaga
     3061 catggacact atctgtcgac gcttgatggc atcgatgatt tcagcggcat tcatatacga
     3121 gtctgcctgc gatggaagaa ccatgtcggc ttcgaggagc gtagcatatg tctcccgtat
     3181 gtccgactcg tagccatcta aagcttcttt tagccggacc ggttcatcca agacaaacag
     3241 ggcgtcctcg gcccagtccg ccaccatcac ccgttccgag tagaaaaacg ggagatactg
     3301 ctcgtcattg tcgaagtagg cccctgtttc gaagcgctcg agatgatgca taaccctttc
     3361 acgcagctga tttcttgtac gagccatcac catgcttgaa ggagacagca tcgacggcgc
     3421 ctgcgcactg tctgccgaga ccgaccggcc cttgcccgca gcaccagcgc ccacccgatc
     3481 aagctcttcc cggatggaca tgcgcgcacg ttccagacgt tcccggtcat acatgaactc
     3541 ccgcgcggga gtgatagtcg cctcacccaa catctcctgt gagagttgag tgtcagggtc
     3601 aaataccctt atggactcta tctcgtcgcc aaagaactct atccgcacag gccggtccgc
     3661 atcattggga aagacatcga ctatgccccc tcgcacactg gcttgcccac ggccgtcaac
     3721 cttgcccaaa atctcgtagc cgctcgcagc caacatgtat accaggccat cgaggtcgca
     3781 gcgttggccg accctgatat cgaacatggc gccccggaaa atctccacgg gaaccatcct
     3841 tctcccgaga gcacgcgccg gcgccaagac cacgcacttt tcgcctctgg ccagagcagc
     3901 catggccttg gcacgagccg cagaaaggtc agcgctcatc ggcgtctcct cataaggcat
     3961 cacatctacc gctgggaaga caaagacccg atcggaaccg atcatagtcg caatatcccc
     4021 cgacaagctt tccgcgtcat gctgtgtcgc ggtaacgacg aaaaccgtcc tccgggcatg
     4081 acaaaacagc cccgatatga atagggactt ctgcgaaccc gaaaggccga caatatgtgt
     4141 agcgcgcgat ccccgctcaa ctcttctcaa agcttccgtg aaagcggtag agtcgagcag
     4201 gaggtcgact attccggtca accccatacc ccccgggaaa gatgacgcat tacaaacacc
     4261 gcaagaccca gctcaattga agcgggtctg cgcggtctcg atgccgtcct ctacaataca
     4321 tagtatggca gaaacggcgt ttataacagc atccgatatg ataggaagtt catcgcgggc
     4381 aaatgtgtca agcacgtgat ggaccgaatc ctcccatggg ggaggccttc ctatgcccac
     4441 gcgcacacgg gcaatattct cagtgcccat atgttctata atagagccca tacccttgtg
     4501 gccgccagca ctcccctttg cccgaattcg tatgcgcccc acctcgagat ccatgtcatc
     4561 gtacacaaca ataagcgact ccgggcctgc gccgtactgc cgggtcgctc ttgctacggc
     4621 gattccgctg ttgttcatgt aggtctgcgg cttcacgatt agcgtatccg cccctacaat
     4681 cgttcccttt gcaacaagac tgagacaatg cgaagcccgc cggaacttgc
//
LOCUS       DAOSAI010000180         4723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11420_length_4723_cov_3.711868, whole genome shotgun sequence.
ACCESSION   DAOSAI010000180 DAOSAI010000000
VERSION     DAOSAI010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11420_length_4723_cov_3.711868"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="PLM83_06850"
     CDS             complement(<1..1650)
                     /locus_tag="PLM83_06850"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HOK71157.1"
                     /translation="MRRFESKYGYFDETAREYVITTPHTPRPWVNVICPGDWGLVLSQ
                     TGGGYSWRTHASLNAITRWSQDIVRDNWGKYIYARDDNSGEIWSLSWMPARRKPDRYR
                     VRHGIGYSVIESENSGISSELTVTVPPGEPLEVWLVELSNDGSEDRELSLFTYFEWCL
                     GASPDWHREFHRTFIETSIEPDASTMWARKRLWEIPNDRGEAWNRPWGYSAFHASSLD
                     AAGFEGDKEAFLGMYGDLARPAAVARGVLSNSHGKWTDAIGSIHVKIKLPAGEKRRVS
                     FLLGAVEAGCEDDARAQIAQYRDPGEAADAMRRASEFWCDLLKPYEVRTPDEAFNIMN
                     NTWLPYQAISGRMWGRTGYYQAGGAYGFRDQLQDSQVFLPLDPSETAKQIKLHGAHQY
                     VDGHVDHWWHPISEIGGGGLYSDDLLWLPFVTIGYLKETGDYALLDEEVPYRDGESES
                     IWQHCIRAIDLSLSRRSERGLPLMLGGDWNDGMNAVGTEGRGESVWVAEFLCTILPDI
                     AHVARRRGEEEVASRYEQAWRELADAVNTHGWSAEGGWYIRA"
     gene            complement(1706..2731)
                     /locus_tag="PLM83_06855"
     CDS             complement(1706..2731)
                     /locus_tag="PLM83_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK71158.1"
                     /translation="MSPTIRDIAEAAGVSTAAVSYVINDKPGVGEETRRRVLKIMREM
                     NYRPNPQARGLAARRTGMIGLMIPDITDPFYVNIVRGVEAKANELGYTLNLCTTHGDA
                     ERERDVAELFASGGVDGVIMMTYKLTPSELKELSEGRAPVVAIDNPEAAGWVPSVLVD
                     NIALGRTATSYLTELGHTRIGFIHGVEGSIASMRRYEGYVQALKSQGAEFSPELVEYG
                     GFTHAGGVEAARVLLSRPQRPTAIFAANDQMALGAMSAAMELGLSVPSDLSIIGVDDI
                     EAAALVVPGLTTLKQPTCEIGACAVGILADLIRSPEGDDEAGASDVGQRVFEARLIVR
                     GSCAPRQ"
     gene            3330..>4723
                     /locus_tag="PLM83_06860"
     CDS             3330..>4723
                     /locus_tag="PLM83_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71159.1"
                     /translation="MNISNTRFNRIAKISIAVIALLTIPVRGFAESKQSSPGIVHARV
                     EADVTGDGINDAILLVGYQSELGGLFCRSHEVVVEDGATQKKATLDLGDLGAGYPGSL
                     SVGELDGAYGSEIVVAIPTGGSGGITNHLIVSARDGNVRMLVDAEELARGPKLDTRSL
                     DHFTIELVDKSKGDRFLLGLIKPDESDEFDPYSEYYSESGKLLQPIDICIDDVSAAEL
                     APIDGKSGLCEFVTYQKVWAIYHANSVGMIRTAWRYVDGELKIASIDVTPVLVPDAYL
                     DYLESIDHSDPADAVEKAISDYKLRFWNAPPAFRELAFAEFRKFHLAIAQAEAESLER
                     IAGIVDAKDSNDVLAAYARLPHIAADKNVSHAYKRAGLTTVYAGEGLWTVQPRAGFYA
                     EQFGTMLTPSVARFVELDDVERNEPWLVDGALVVSMKELSRRTAGWEAYLESNPYSLF
                     ADEARIRFNAAARA"
BASE COUNT         1089 a         1400 c         1202 g         1032 t
ORIGIN      
        1 ggcgcggatg taccatcccc cctcagcgct ccagccgtgg gtgttaaccg cgtcagcaag
       61 ctcgcgccat gcctgctcat aacggctagc cacttcctct tcaccgcgac ggcgcgcaac
      121 atgcgcaata tccggaagaa tggtacagag aaactccgcc acccacacac tctctcccct
      181 gccctcggtt cctacggcat tcattccgtc attccagtcg ccaccaagca tgagcggaag
      241 ccctctctcg cttcggcgcg acaggctgag gtcgatagcg cggatacagt gttgccaaat
      301 actctcggac tctccgtcgc ggtatgggac ctcctcatcg agcaaggcat agtcccctgt
      361 ctcctttaga tagccaatag taacaaaagg aagccaaaga aggtcgtcgg aatagaggcc
      421 gccgccgcct atttcagaga tcggatgcca ccaatggtcc acatggccgt caacatactg
      481 gtgcgccccg tgcagcttta tctgttttgc cgtttcagaa ggatcgagtg ggaggaaaac
      541 ctgactgtct tggagctgat cacggaatcc ataagcccca cctgcctgat aatacccggt
      601 cctaccccac atccgcccgg agattgcttg atagggaagc caggtattgt tcatgatgtt
      661 aaatgcctcg tccggcgtcc taacttcgta tggtttgaga agatcgcacc aaaactccga
      721 ggcacgccgc atggcgtctg ccgcctcacc cggatcacgg tattgtgcga tctgagcgcg
      781 ggcgtcatct tcacagcccg cctccacagc accgagcagg aaggacacac gcctcttttc
      841 accggcgggc agcttgattt tcacatggat cgacccgatt gcatcggtcc atttgccatg
      901 tgaattggac aaaacgccac gcgccaccgc tgcgggcctt gccaaatccc catacattcc
      961 gagaaaagcc tctttgtcgc cttcgaaacc agcagcatcg aggctcgatg catggaacgc
     1021 cgaatacccc cacggcctgt tccaagcctc tcccctatca ttggggatct cccacagtct
     1081 cttgcgtgcc cacatggtgc tcgcatccgg ttcgatagag gtctcgatga aggtcctgtg
     1141 aaactcacga tgccagtcgg gcgatgcgcc caaacaccat tcgaaatacg tgaaaagcga
     1201 gagctcacgg tcttcactgc cgtcattcga aagttcgaca agccacactt ccagaggctc
     1261 gcctggcggc acggtgaccg tgagctcact tgaaataccc gaattctcgg attctatgac
     1321 agaataacca attccatgcc ttacgcgata tcggtcgggc ttgcgccttg ccggcatcca
     1381 cgaaagggac caaatctcgc ctgagttatc atcacgcgcg tatatgtact ttccccaatt
     1441 gtcgcgcaca atgtcttgag accagcgggt gatggcgttg agcgacgcat gcgtgcgcca
     1501 gctgtaaccg ccgccggttt gggaaaggac aaggccccag tctcccggac atatcacgtt
     1561 cacccagggc cttggggtat ggggagtcgt tatcacgtat tctcgcgccg tttcatcgaa
     1621 gtagccgtat ttcgattcga atctcctcat atgactttca gccgcctctc atcgcttctg
     1681 tatgtaaagg ttcccgccgt caatcctatt gccggggagc acatgatccg cgaacaatca
     1741 acctagcctc gaatacccgt tgaccaacgt ctgaagcgcc tgcctcgtca tcgccttcgg
     1801 gactacggat cagatcggca aggatcccga cggcacacgc cccaatctcg cacgtaggct
     1861 gtttcaacgt tgtgaggcca gggactacga gagccgccgc ctcaatatca tcgaccccga
     1921 ttatggatag atccgaaggc accgaaagac caagctccat tgcagcgctc atcgctccga
     1981 gcgccatctg atcgtttgcg gcaaaaatgg ctgtaggccg ctgtggacgg gacaggagta
     2041 cgcgcgctgc ttcgactccc cctgcatgcg tgaacccccc atattcaacc aattccgggc
     2101 taaactcagc tccctgcgac ttcagcgcct gcacatagcc ttcataacgc cgcatagacg
     2161 caatcgaccc ttcaacgcca tggatgaatc ctatccgtgt gtggcctagt tctgtcagat
     2221 aggatgtagc ggtccgcccc agcgcaatat tgtcgacgag tactgatggc acccaccctg
     2281 cagcctcggg gttatcgatg gccactacgg gagcccgccc ctcgcttagc tccttaagct
     2341 cagagggcgt gagcttgtat gtcatcatga taaccccgtc cactcctccg ctggcaaaga
     2401 gctctgccac gtcacgttcg cgttcggcat ctccgtgggt ggtgcacaga ttcaaggtgt
     2461 acccgagctc gttggcttta gcctccacgc cgcggacgat gttgacatag aatgggtcgg
     2521 taatgtcggg gatcatgagc ccaatcattc ccgtgcgtcg ggccgcgagc ccccttgcct
     2581 gcgggttagg cctgtagttc atctcgcgca ttatcttgag caccctgcga cgagtctcct
     2641 cgcccacacc gggcttatcg ttgatgacgt aagagactgc cgccgtcgat acgccagctg
     2701 cctcggcaat gtcacggatc gtgggagaca taaccaagcc accttccaaa atcaagattg
     2761 ggtagccgaa ccgcgaaaat cctcgcaatc gtatcttcaa ggaacgaata attctccact
     2821 taaccgatta agcacgagtt ccaatagaag gcgggcattc attcccgccg ctcgccccgg
     2881 ctctgtgcca gggacatcct accaaatcca gccatcctag catactcgag catgcagtta
     2941 aagcagcctc gtgttgtctt ggacaacctt ctgaggacaa cttaaccggt taaggttaga
     3001 atagcaaaca tagagttgca tgtcaatact ggtcaaggaa aaccttgcta taggaatggg
     3061 aggaacatgt tgaaaggtcc tgtcgacaga tccgtgcaaa tgctctattt tagtgattat
     3121 tgcttgactg ggagtgttct tagaattcga gatcgcctac acgcctaaac cgcccaagcc
     3181 cagccgagat ttaaaagcct cctccgcccg ttattgggca ggttgctttt ccataacata
     3241 ccctgcattt tgcatgcaat tgtcgatata atctaattgc agcgtgcttt gcggccgcgg
     3301 caaaatcttc tatgtcggag tgagaaaaga tgaatatcag caatacccga ttcaaccgaa
     3361 tcgcaaagat atccatagcc gtaatcgccc tcctaaccat acctgtgaga ggattcgccg
     3421 agagcaaaca gtcatcgcca ggcatcgtcc atgctcgtgt tgaagcagat gtcacaggcg
     3481 acggcataaa tgacgccatt ctccttgtag gttatcagtc tgaacttggg ggtctgttct
     3541 gccgttcgca tgaagtcgta gtagaagacg gcgcaaccca aaagaaggca acccttgacc
     3601 taggcgatct gggcgccggg tatcccggtt cgctctcagt gggcgagctc gacggggcct
     3661 acggttccga gatcgtcgta gcaatcccaa ccggcggttc gggagggatc accaatcacc
     3721 tcatcgtatc cgcgagagac ggtaatgtgc gtatgctcgt cgacgcggag gagcttgccc
     3781 gcgggcctaa gcttgatacc cgcagtctgg accactttac tatagaactt gtcgacaaat
     3841 ccaagggcga tcgatttctc ctcggactca tcaagccaga tgagtctgac gagttcgatc
     3901 cgtacagtga gtattactca gaatccggaa aactgcttca gcctatagac atttgtattg
     3961 atgatgtctc cgcagcagag ctcgcgccga tcgacggcaa gagcggactg tgcgagtttg
     4021 tgacatatca gaaggtatgg gcaatctacc atgccaactc tgtagggatg attcgcaccg
     4081 cttggagata tgtcgacggc gagctcaaga tcgcctcaat cgacgttact ccggtcctcg
     4141 tgcccgacgc ataccttgat tacctcgaat ccatagacca ctcagatcct gctgacgctg
     4201 tggagaaagc catttctgat tacaagctca ggttctggaa cgctcctccg gcattccgag
     4261 aactagcctt cgcagaattt aggaagttcc acttggcaat agcacaagca gaagctgaat
     4321 cactggagcg catagcggga attgtcgatg cgaaggactc aaacgacgtg ctcgccgcct
     4381 acgcccgtct tccccacatc gccgcagaca agaacgtgtc acacgcatac aaacgtgccg
     4441 gccttacaac ggtttacgct ggcgaagggc tgtggactgt acagccccgt gccggattct
     4501 acgcagaaca gttcgggacg atgctaactc cgtcggtcgc gagattcgtc gagctcgatg
     4561 acgtggaaag gaacgagcca tggcttgtcg acggcgctct cgtagtctcc atgaaggagc
     4621 tgagtcgtag aacagccggg tgggaggcat atctcgagag caatccatac tcactttttg
     4681 cagacgaagc aaggatccga ttcaacgcgg ctgcaagagc gtt
//
LOCUS       DAOSAI010000181         4697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11506_length_4697_cov_3.693882, whole genome shotgun sequence.
ACCESSION   DAOSAI010000181 DAOSAI010000000
VERSION     DAOSAI010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11506_length_4697_cov_3.693882"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLM83_06865"
     CDS             <1..1011
                     /locus_tag="PLM83_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71160.1"
                     /translation="TGAIVDKLGAVGPVMMSGARAGELMQIVAPPEIVKALADGSVKL
                     MQTPTGATSSVVAEGSSRIVGQVRLAPADVAPIVAPVVLYQILNAIAGAHQLSKINAR
                     LDSLQRAIDSVIFRQQANSYARLYAAIDALQELDREYCAIGMFTDDMRWRLSIATNDI
                     QAVNYESSFIIGRFKRMSEDLIRSSHKRQGAIQANTMLNEESNVYMLDAAIYVTAVKA
                     GLMAEQMWIRHDLQFVPEYVGYRLADLNCEIEELKETVPPMRYIEELQDHADSCLREM
                     RSLQRWTSSRLSKEIRSRSEQFASMQEDDSRQYTAQSLFIWEDDKGEIKALSGVEAID
                     TK"
     assembly_gap    1564..1663
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1694..2267)
                     /locus_tag="PLM83_06870"
                     /pseudo
     CDS             complement(1694..2267)
                     /locus_tag="PLM83_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
     gene            complement(2407..3183)
                     /locus_tag="PLM83_06875"
     CDS             complement(2407..3183)
                     /locus_tag="PLM83_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOK71161.1"
                     /translation="MPEHDSTREWKSFFDIYAPYYMDEPFTLNTCREVDFIIEIMALE
                     PGASVLDVGCGVGRHSVELAKRGYQVTGVDISEGMLHEAARRAEQARVDVKWLRQDAT
                     ELKLDRTYDACICLCEGAFGLLNSGENAADRDISILRGIASALVPKGPFLLTALNALR
                     TIRMYSDDDVAAGRFDNIALCQIHPIGSFIADDIRNELPENILSSVIREKAFTPMELT
                     LMLNITGFSVEHIWGGTAGNWGRRPLLMDEIELMVMARLD"
     gene            complement(3629..>4341)
                     /locus_tag="PLM83_06880"
     CDS             complement(3629..>4341)
                     /locus_tag="PLM83_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOK71162.1"
                     /translation="CAARLPVYVLFATAFFESRRGLVVFSLYALGVVLAFIFAKVLSL
                     FVEPDDEATFVLELPPYRMPRISGVLIQTWERGKEFLEKAGTVILLSVVLVWALSNLP
                     PGVDPGSPDSLIGRIGKAIAPILAPAGFGQWQAAAALLFGVIAKELIVGTLGVLYGTT
                     GEAGLIAAIRASWTPLAAYSFMVMALTYIPCTATIAAIRRETGSRRWTAFAVAYTLVL
                     GWTLAVIIYQVGRLFGLA"
     assembly_gap    4342..4351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4352..>4697)
                     /locus_tag="PLM83_06885"
     CDS             complement(<4352..>4697)
                     /locus_tag="PLM83_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOK71163.1"
                     /translation="SGLFATFGTWLAGSLEAAGTPNIVIGLLVEGVVGGLGAVLEFAP
                     PIFMLFLMISLLEDVGYMARAALLSDRLMRSVGLHGKAFIPMILGFGCNVTGILATRS
                     LDSKRDRLISILI"
BASE COUNT         1047 a         1180 c         1292 g         1068 t
ORIGIN      
        1 actggagcga tagtcgacaa gcttggcgcg gtgggaccgg tcatgatgag cggggcgaga
       61 gccggcgaat tgatgcagat tgttgcgccc cccgagattg tgaaggcttt ggcggatgga
      121 tcggtgaagc tgatgcagac gccgactggg gctacaagca gcgtggtagc tgagggatcc
      181 tcgagaatag tgggccaggt taggttagcg ccggcagatg ttgcgccaat agttgctcct
      241 gttgttctct atcaaattct aaatgccatt gcaggtgcgc atcaactgag caagatcaac
      301 gctcgtttag acagccttca aagggctata gattccgtaa tcttccgtca acaggcgaac
      361 tcatatgccc gactctatgc cgccatcgat gcgttgcaag aactggatag agagtattgc
      421 gctatcggaa tgtttacgga tgacatgcgc tggaggcttt ctatagctac gaatgatatt
      481 caggctgtaa actatgaaag cagctttatc attggccgct ttaagcgcat gagcgaagat
      541 ctgataagat catcccataa aaggcagggt gcgatacagg cgaatacaat gctgaacgaa
      601 gagtcgaatg tgtacatgtt ggatgcggcc atatacgtga ctgcggtcaa ggcggggctc
      661 atggctgagc agatgtggat aaggcacgat cttcagtttg tccctgaata tgttgggtat
      721 aggcttgccg acttgaattg cgagattgaa gagctgaaag agactgttcc gccaatgcgt
      781 tatattgagg agcttcagga tcacgcggat agctgtttgc gggaaatgcg gtcgttacaa
      841 agatggacca gctcgcggct gagtaaggag atacgaagcc gctctgagca atttgcgtca
      901 atgcaggaag atgattctcg gcaatatact gctcaatcgt tgttcatatg ggaagacgat
      961 aagggcgaaa tcaaagcctt atccggagtg gaggcgatcg atacaaaata gacgttaccg
     1021 ctgccacagc gcttcggttc ggctacagtt cgtctgcttc gtcgatgtta catgcgatgc
     1081 aaaactccgc agtgtgatga ggaccctctg acggttggct attcgacggg cactgcaacc
     1141 ggatcataat tctttttgcc gtattgcata gaaaggccat tgatataata gtgtgtgccg
     1201 cttcccagct ggacgccatg ggcagaatcg atgtggccag agcaaaagga atgccaaatg
     1261 ggatggcgag ttggctcccg atcatcctat ttccaaagat cgggatggat gtctcttggt
     1321 caagccctgc gtcttttgga atatcgtagt cctcacggaa tggcgggcta cgcgaatctg
     1381 cccaaaaata gtgaacgagt ctgatcgtat taggaaagtc aatgccacaa aatgtttcat
     1441 gtgaaacatt tgactatgca ttatgcacat agatgtccag aaaactctgc ttttcatggc
     1501 ataaactcgg tctgctcccg tctggcacct gggcgagttg ttggcctcag caaggagctg
     1561 aggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctaacat gagcgttcgg
     1681 ggacgctgca cggtcagaac gaccagtctt ccctggcgaa gccccagcct gtcttatcca
     1741 tctccttgag gaatctcgcc agataatctg agagagctcc tatcatcttc tcgccttgct
     1801 cccgcgtatc cccggtcgct ttggtcctgg agatctgggg tatcgtggtc ttgatgctca
     1861 cttcagccgg gctggccggc cactcgcccc gtgctgcatc catatcaacc aggtcaggca
     1921 cgatcgccag ccctgcagct gtttcggctt tgccggcgtg cccaaggccg ttctctaggc
     1981 caaaggttcc cggcggacac aggcgcggcc ccgatatcca ccaagcgtac gacactgccg
     2041 ccttggctga aggatactct cggccaaggc gtctcaaggc gatgtcgatg gcagagttgt
     2101 tcccgccatg gccgtttatg aggagaaaac gatcgatgcc ttggacgata caggactcca
     2161 gtaattcgaa gactacttgg gagagggttt caggacggag acttaagtta aagggatacg
     2221 gcatcagcgg tccgttctgc tatggcgtat gcggcgtagg tgtccatgcc catgggaaga
     2281 tgatagccgt ggttctcggt tgcgccgata acaagaatag ctttgttgaa gtaggatgct
     2341 tcgaacctgt gccggttgag cttttccagt ctgaattcgt ttacctttga catctgcgct
     2401 gcctcctcag tcaagcctgg ccatgaccat taactctatc tcatccatca aaagaggacg
     2461 acgcccccaa tttcctgcag tgccgcccca gatgtgctcc acgctgaacc ctgttatgtt
     2521 cagcatcagc gtcagctcca ttggggtgaa ggctttctcc cttatcacgg aggataatat
     2581 gttttccggc agttcgttgc ggatgtcgtc ggctatgaac gagcctatgg gatggatttg
     2641 gcacaaggca atgttgtcga atcttcctgc ggctacatca tcatcgctgt acatgcgtat
     2701 agtcctgagg gcgttgagag cagtaagaag gaaggggccc tttggaacca gggctgaagc
     2761 gatgcccctc aatatggaga tgtcgcggtc tgcggcgttt tcgcctgagt tgaggaggcc
     2821 gaaagctcct tcgcacaagc atatgcatgc atcgtaagtg cggtccaatt tgagctcggt
     2881 ggcatcttgg cgcagccact tcacatcgac tctcgcctgt tctgctctgc gggcagcctc
     2941 atgaagcatt ccttcggata tgtctacgcc agtgacctga tatccccgct tggcaagttc
     3001 aacgctatgc cgtccgacac cgcaacctac gtcgagcacc gatgcgccag gctcaagggc
     3061 cattatttct attatgaagt caacctctct gcaggtgttg agggtgaaag gctcgtccat
     3121 gtaatagggc gcatagatgt cgaagaagct tttccattca cgggtcgaat cgtgctcagg
     3181 caaagctatt cctcctcgcg ttatctttac cctgtttgtt ctgtgtgcgc aggtctcttc
     3241 ctgcatcgcc ctgacgatgt tgacaccgtt gtgccaaggg caatccggga ttcccctgag
     3301 ctcgatgagc acctagcgga tgcaaccggg cgttttgaat ggtgtatcgc cgtagaggtt
     3361 ttattagagt atggagtcaa gctcgacaga aagttgatcg gaacgagaac cctagggata
     3421 agcgcggcaa ggtttggttt gattgggcca gtgccagatg cgcctgggat gcttcgtggc
     3481 ctccgagcta tgtgccaggc ataatctctc aggcgtttgc tgatgatccg aagtactgtt
     3541 ggcgccttcg ggccgctgtg catcgacgga ggaggccaga ggcggcagcc gcctctggct
     3601 cggttacgag catagcataa cgaactgctc aggccagccc aaaaaggcga cccacttgat
     3661 agattatgac tgcaagcgtc cacccgagca cgagagtata tgccacggcg aaagccgtcc
     3721 atcgccgaga acctgtctcc ctgcgtatgg ccgcaatcgt ggctgtgcac ggtatgtatg
     3781 tgagggccat aaccatgaat gaataggccg cgagcggggt ccagctggct ctgatcgcag
     3841 cgatgagtcc cgcttcccct gttgtgccgt agaggactcc cagggtgccg acgatgagct
     3901 ccttggctat caccccaaag agcaaggccg ctgctgcttg ccattggccg aagcctgcgg
     3961 gggcaagaat gggagctatt gcctttccga ttctccctat cagcgaatcc gggctgccgg
     4021 gatcgacgcc tggcggcaga ttggacaatg cccataccag gacgactgag agcaggatga
     4081 ctgtgcctgc cttctccagg aattcctttc ccctttccca cgtctgaatc aggaccccgg
     4141 atattctcgg catgcgatac ggcggcagtt caaggacgaa tgtggcctcg tcatccggtt
     4201 ccacaaatag gctcaatacc ttggcaaaga tgaaagctag cacaactccg agggcgtaca
     4261 aggagaagac aaccagtccg cggcggcttt cgaagaaagc tgtggcgaac agtacataca
     4321 caggtagacg cgctgcgcag gnnnnnnnnn naatcaatat ggatataagg cggtcgcgct
     4381 tgctgtccag gcttctggtg gccagaatgc ccgtgacgtt gcatccgaac ccgaggatca
     4441 tcgggatgaa ggctttcccg tgaagcccga ctgaccgcat cagcctgtct gatagcaatg
     4501 cagcccttgc catgtagccc acgtcttcaa gaagtgagat catcaaaaaa agcatgaata
     4561 ttggcggtgc gaactccaat acggccccta gtcccccgac tacgccttct acgagcaagc
     4621 caataacgat attgggtgtt cccgccgctt ccagagaacc tgcaagccag gtgccgaatg
     4681 ttgcaaataa gccgctg
//
LOCUS       DAOSAI010000182         4687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11539_length_4687_cov_4.128670, whole genome shotgun sequence.
ACCESSION   DAOSAI010000182 DAOSAI010000000
VERSION     DAOSAI010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11539_length_4687_cov_4.128670"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..979)
                     /locus_tag="PLM83_06890"
     CDS             complement(68..979)
                     /locus_tag="PLM83_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK71164.1"
                     /translation="MQQHKGFSMEQKKARWGWIFVAPTLAFFLVFSFYPIGNAIYMSF
                     FKKNLLSLKPPEFVGWGNYKYLFSSDMFWQSVANTAIFTVGTFIPLVVLSLVLAAFIM
                     SRTKLQKFLQMALYSPAALSSVVAALIWLLIFDPRGLANQALNFINGTSGVNYHWLSN
                     ANMLRLSTIILYFWKYIGYFTIVFIAGIGGIPDYLHEAALIDGANRWQDFWHITFPLL
                     KPTTLLVSVMVMIQCLKTFSTQYLFTTSGAPTRPIDVITLSIYNTAIKDHNIGRASAM
                     SVLLFLVMLVLTWLQFRISRTEEVTYM"
     gene            complement(1058..2338)
                     /locus_tag="PLM83_06895"
     CDS             complement(1058..2338)
                     /locus_tag="PLM83_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK71165.1"
                     /translation="MKRSTWLLAVAIIVSLSFGVGAAKPIELTYWTHTDDNRTEIENR
                     YIAEFTKMYPNVRVKRVVNEASKMGDIVLTAFSANNAPDLFNLPIEQEYGYMKNYRVA
                     PVDCKAIGVKNYDELKSQYIKGTFDAVTMGGKIYGLPLEITNWCIYINKKVFRDAGLD
                     PEKDYPKTWEDMADISDKIAIRNGDILVRRGFDFRYPYYLVSFLPMVEQLGGKLLSDD
                     GKTAIVNDKAWLQVLEYMREWGPHGRNLGSPTYAAPRKIWNKDNNDIAMCLSGFYQQG
                     RLQNDNPAFFESKEWMVVPFPVFKNAVNDVACSFYGHYLMVNAQAPKEKQEMAWKLAD
                     YMLSHSMEYLVKVNLIQPRKELLTSKTFKNLPYTEVFMADMNRAHPVFLHENGYQFER
                     FIKEAVEGVMLSKVPPKEALNTLRNRIQEVLDNE"
     gene            2943..3644
                     /locus_tag="PLM83_06900"
     CDS             2943..3644
                     /locus_tag="PLM83_06900"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOK71166.1"
                     /translation="MEFLANQGKRLSVEIDGRTYMRIPIKTHVVVADDDIVDVVGRYA
                     EPLLCTGDMLVVSEKIVAITQHRAYPVDQIEPSRLARFLSRFVYKSPYGIGLASPCTM
                     ELAIREAGAPRIVAAACVAGVGKLFGAKGVFYRICGANVAAIDGPCDYTIPPYDRYAI
                     LAPSKPKDVAQSLSDATGMPVAIVDANDLGVSVLGLSAGCPEPELIKKILADNPLGQS
                     CEQTPIGIVRQAKDI"
     gene            4116..4502
                     /locus_tag="PLM83_06905"
     CDS             4116..4502
                     /locus_tag="PLM83_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOK71167.1"
                     /translation="MGMPPLGDQELELLRFITEHSPITVREAYENYGKDRGLARTTIL
                     TMMERLRNKGYLTRSEGKGANEYSACLTRSALMRGVVRGFVEETLGGTLSPFVAYIAE
                     EASVSPQELEELKRLVEELESREEEN"
     gene            4499..>4687
                     /locus_tag="PLM83_06910"
     CDS             4499..>4687
                     /locus_tag="PLM83_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71168.1"
                     /translation="MRGCLQYLFQISSQYWASSMWRAFYQGSLALAFVWAVCKAFPHI
                     PARVRCWLYRLGLAKLFLQ"
BASE COUNT         1121 a         1104 c         1284 g         1178 t
ORIGIN      
        1 tttagcataa ttcatgtcaa tccccctgat cccagagcat ctagctgatc gccctgtttt
       61 gatatttcta catgtaagtg acttcctcag tcctagatat cctgaattgc aaccatgtta
      121 ggaccaacat gacaaggaaa agcagaacac tcatggcgct ggctctccca atattgtgat
      181 ccttgattgc agtattgtag atactgaggg taatgacatc aatgggtctg gtgggcgccc
      241 cggatgtcgt gaagaggtat tgcgtgctaa aagtcttgag acactggatc atgaccatca
      301 ctgaaaccaa gagagtggtc ggcttcagta gcggaaatgt gatatgccag aagtcttgcc
      361 atctgtttgc tccatcgatc agagctgcct cgtgcaagta gtctgggatg cctcctatgc
      421 cagcaatgaa tacgatggtg aaatagccga tgtatttcca aaaatacagt attatcgttg
      481 acaaccgcag catgttcgcg ttagagagcc agtgataatt gactcccgat gttccattaa
      541 taaaattgag cgcttggttt gccaggcccc gaggatcgaa gataagcagc cagataaggg
      601 ccgccacaac cgacgaaagc gcggccgggg agtatagcgc catctgaagg aatttctgca
      661 actttgtccg cgacatgatg aaagcggcca atacgaggct gagaacgacc aggggaatga
      721 atgtgcccac tgtgaatatg gctgtattgg ccacagactg ccagaacatg tctgacgaga
      781 ataaatactt gtagttgccc caaccgacga attcaggcgg cttgagcgac aacagattct
      841 tcttgaaaaa gctcatgtag attgcattgc caatggggta gaagctgaag actaagaaga
      901 acgccaaagt cggagcgacg aatatccatc cccatctggc ctttttctgt tccatactga
      961 atcccttgtg ctgctgcatc gaatctcccc ctcaaggaaa tctcggaagc taaagtccgg
     1021 gttgcaagaa cccggacttc agcttgcgct gctactgcta ttcgttatcc aggacttcct
     1081 ggatacggtt gcgcagggta ttaagggcct cttttggagg caccttggac agcataacgc
     1141 cttcaactgc ttcctttata aatctttcaa actggtaccc gttttcatgg aggaagaccg
     1201 gatgtgcccg gttcatgtct gccatgaaga cttcagtgta aggaaggttc ttgaacgtct
     1261 tcgacgtcag gagctctttg cgcggctgaa tgaggttgac ctttaccaga tactccatgg
     1321 aatggctgag catgtagtca gctaacttcc acgccatctc ctgcttttct ttgggcgcct
     1381 gggcgttgac catcaggtaa tggccataga aggagcacgc cacatcgttg acggcgttct
     1441 tgaagactgg gaacggaaca accatccatt ctttgctttc gaagaatgca gggttatcgt
     1501 tctgcaaccg gccttgctga tagaacccgc tgaggcacat ggctatgtcg ttgttgtcct
     1561 tgttccagat tttcctggga gcggcgtatg tggggctgcc gaggttgcgt ccgtggggac
     1621 cccattccct catgtattcc agcacttgca gccatgcctt gtcattgact atggctgtct
     1681 tgccgtcatc actcagcaac ttcccaccca actgctcaac catggggagg aaagacacga
     1741 ggtagtacgg atagcggaag tcgaacccgc gccgcacgag gatatcacca ttgcgtatgg
     1801 caatcttgtc ggagatatcg gccatgtctt cccatgtttt ggggtagtct ttctcgggat
     1861 cgagccctgc atcgcggaag accttcttgt ttatgtagat gcaccagtta gtgatctcta
     1921 ggggaaggcc gtatatcttc ccgcccatgg tcactgcatc aaaggtgcct ttgatgtact
     1981 gggacttcaa ttcgtcgtag tttttgacgc caatggcctt gcagtcgaca ggagcaacgc
     2041 ggtagttctt catgtagccg tattcttgtt cgattggaag gttgaatagg tctggagcgt
     2101 tattggctga gaaagcggta aggacaatgt ctcccatctt gctagcttca tttacgactc
     2161 tcttgacgcg cacgttaggg tacatcttgg tgaactcagc gatatacctg ttttcgatct
     2221 cggttcggtt gtcgtcagtg tgggtccaat aggtcaactc aataggcttt gcggcgccga
     2281 ctccgaagga cagggatacg atgatggcaa cagcgagcaa ccatgtagac cttttcatga
     2341 acaactcctc ctctagcgtc tttgttgggc agccaaccca tgtagtgtct gcggcgcgtc
     2401 acctccatgc caggactaca ggaaacccca gcgtgatcgt tgtcattaga aaccaactat
     2461 aaacaaaatt cgtcattgaa gtaagtagtc ctgcgtccaa ctgcaaatcc gacacgtttt
     2521 tgctatatgt tagtcagtaa gtgaaggcgc ctggcgtggc atagaccttg tgccgcctgg
     2581 gggacttgat gataataaag tatctcagct tgccgccacc ggctacgatt tgcggcaggt
     2641 ttgcatcgct ccatgaatta tcgccactca ggctttgact gcaagcgtga tgagcatgtt
     2701 caaatcctcg ttgagacatg atgcagactt gtagaaaggc ttctcaaggc ttgcgttatt
     2761 ctgatcggcc gcaaagccag attcgacagc caagacagtt gactacaaca ctagtctgtg
     2821 ctaatgttga ctacagatgt aggcacactg gtgactgatg cggggggcca gcggagttgc
     2881 agctagaaag ctggattcag aagtcctatt tgcatggagc agcaaagggg atgttgaaag
     2941 tgatggagtt tttggcgaat cagggtaaga gactatcggt ggagattgac gggcgcacct
     3001 atatgcgtat tccaatcaag actcatgttg tcgtcgcaga cgacgatatt gtggatgtgg
     3061 ttgggaggta cgctgaacct ctgttgtgta caggcgacat gttggtagtg agcgagaaaa
     3121 tcgtagctat tactcagcac agggcgtatc cagttgacca aatcgaacct tcgcggcttg
     3181 ctcgttttct ctctcggttc gtgtataaat cgccctatgg aatcgggctg gcgagcccgt
     3241 gcacgatgga gctggctata agggaagctg gcgcgccgag gatagtggcg gcggcgtgtg
     3301 tggcaggggt tggcaagctt ttcggagcta aaggagtctt ttaccggata tgcggcgcaa
     3361 acgtggccgc aatcgacggc ccatgcgact atactatccc gccctacgac aggtatgcga
     3421 ttttggcccc gtcgaagccc aaagatgttg ctcagtcgtt aagcgatgct actggtatgc
     3481 cggtggcgat tgtggatgcg aatgacttgg gcgtttccgt acttggatta tcggcgggat
     3541 gccccgagcc ggagcttata aagaagatcc ttgccgacaa tccccttggg cagtcatgcg
     3601 agcagactcc cattggaata gtgcgccagg ctaaggatat ataggttata gcggaacttt
     3661 caagattcga cgttttctcg gcgttcaaag cggcctgaag aaacagtgat agccgtggtt
     3721 ggatgtgtgt ttcttgtagg tgacgacgat cggcaataaa gggagaacaa cgtcaggcgc
     3781 ccacctcgtg gccaagacag aatgtggcaa gccgagttga ctgcttagcg attacacaga
     3841 gacagacagc gggcaatgca catcgattag cacctatctc aatttaatgt gggcacgtgt
     3901 gccatggtgc tcttttggct gctttgggga aaacacaggg tttgcgttaa cccccaaact
     3961 aggcccgcgt gaccttagaa gaggtgacgg cagttacggt tgctacaatg cccacgtgca
     4021 ctcggttgca gttgacgccc tctgattcca tgcgtatact gactacagat atagttagga
     4081 gtgctttaag acgacgagat ggggggtatg ggcatatggg aatgcctcct ctgggcgatc
     4141 aagagttgga actgctacga tttatcaccg aacattcgcc gatcacggtg cgtgaggctt
     4201 atgagaacta tggaaaggac cggggccttg cgcgaaccac aatcctcaca atgatggaaa
     4261 gactcagaaa caagggttat ctgactaggt cggaggggaa aggggcaaat gaatattctg
     4321 cctgcttaac ccgttcggcg ctcatgcgcg gagtggtgcg tggattcgtg gaagagacgc
     4381 ttggaggcac tttgtcgcct tttgtagcat atatcgccga agaggccagt gtaagtccgc
     4441 aggaacttga agaactaaag cgacttgttg aggaactcga atcgcgggag gaagagaatt
     4501 gaggggctgt ctgcagtatc tgttccaaat ctcatcccaa tactgggcaa gttccatgtg
     4561 gagggctttc tatcagggga gcctggcgct cgcgtttgta tgggctgtat gcaaagcctt
     4621 cccgcacatc cccgcccgtg tcagatgctg gctataccgt cttggattgg ccaaattgtt
     4681 tcttcaa
//
LOCUS       DAOSAI010000183         4667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11600_length_4667_cov_3.552038, whole genome shotgun sequence.
ACCESSION   DAOSAI010000183 DAOSAI010000000
VERSION     DAOSAI010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11600_length_4667_cov_3.552038"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLM83_06915"
     CDS             <1..426
                     /locus_tag="PLM83_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOK71169.1"
                     /translation="NAQEIDALAKYGRFLGFIFQTVNDINNLTGFDSNTKGASLGDLE
                     EQRVGFPMVGLAMQVGEERLAALSAQARRDAEGAKQAGIEMSVLLEEGPVRQWLEGIL
                     EDLQQEAVRALSAFPESFERHALESIGKDMFESWFWEPD"
     gene            428..1138
                     /locus_tag="PLM83_06920"
     CDS             428..1138
                     /locus_tag="PLM83_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71170.1"
                     /translation="MAKKNNGARPLAESAIAMALAGIMWHVFAPMPGIGPYFKYMSAS
                     LIAIVGSRHGRHWSTMTAVGLNALVGLAYGPKELLDSLLLVTPIGLMLPTGGPDGDEA
                     ADDRREFGLLNTETDKSKAEASFRRHDTWSAGKWWAMILLSYALLYPARVWALATLMG
                     AKVSDVVGSMAATSYHVFERFWALANISADTVVGAAVDWMRIILPVVGMGYGAMLYLV
                     NRPAYFGGLAILKRTRVG"
     gene            complement(1116..1589)
                     /locus_tag="PLM83_06925"
     CDS             complement(1116..1589)
                     /locus_tag="PLM83_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71171.1"
                     /translation="MKIESLKIVIDSDELEAALKCIEMPQNVRIRGAHFEPDELVVDL
                     SVLAPIPLYPSFHVCVIQAAGPTVSFQVSTPLMTGLVDRFISNATTNLPDGVAYLGKG
                     IVSLDIPTLANGTVSSINIGGIQFEDGHATVAVDEIDIDISAITQLSSVNRPGSV"
     gene            complement(1586..2572)
                     /locus_tag="PLM83_06930"
     CDS             complement(1586..2572)
                     /locus_tag="PLM83_06930"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HOK71172.1"
                     /translation="MFGYTKPYGQHSLANAANVPQQGAQACRNLAAKETCSYKAHYCG
                     LCFALSRQYGPLWRACANFDSTLLLLLMSAQQPDAPTLTKAFCPLGAPRRYCVIDGDE
                     LFMKIGSAFTVAMVDLKSQDAIIDGARAARPARLLGRRAFARAERVLAENHFDASSLD
                     EIQKRQGKLESMAVENPLSVSFDDLAAPTGHGLAKVFEYTADLAGLRPNAEPLRRIGN
                     AAGKIIYAADCLEDLETDLARGRFNGLAACSMVVPGTLILTGEASAGLKYLLDRCRAE
                     IAYALRELETVRYRAIIENILLTSLPLRASEVLASRTESGHEPNHHKVDDIG"
     gene            2958..3458
                     /locus_tag="PLM83_06935"
     CDS             2958..3458
                     /locus_tag="PLM83_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK71173.1"
                     /translation="MEAILTRRSIRKYTSEPVTDDEVKQLLRAAMHAPSAGNQRPWEF
                     IVIRDKETLNEITGIHKYAQMLKQAQVAIVVCGDVKKQKYDGFWVQDCSAATQNMLLA
                     ATALGLGAVWLGVHPVVERVEDLRKILGIPVNVVPLSIVSVGRPGEEREAADRYDESR
                     IHMHRW"
     gene            complement(3618..3962)
                     /locus_tag="PLM83_06940"
     CDS             complement(3618..3962)
                     /locus_tag="PLM83_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOK71174.1"
                     /translation="MAEKISEKTKKMLVHIYALFLALGDRRVPWHTKAVAAITVGYAF
                     SPIDLIPDFIPVLGQLDDLLLIPEGLMNELRLKAQSMTDGEKPTSWIAGAVVVSIWAL
                     AIIAIVLKIIRR"
BASE COUNT          985 a         1149 c         1450 g         1083 t
ORIGIN      
        1 aatgcgcagg agatcgatgc gcttgcgaag tatggaaggt ttcttgggtt catttttcag
       61 acggtaaatg acatcaacaa tctcacaggg ttcgattcaa acacaaaagg cgcttcactg
      121 ggcgacctcg aggaacagag agtcggattt cccatggtcg gccttgcgat gcaagtaggg
      181 gaggaacggc tggcggcgct ttcggcccag gcgcggcgag acgcggaagg ggcgaagcaa
      241 gctgggatcg agatgtcagt gctcttagag gaagggcctg ttcgccagtg gctcgagggc
      301 attttggaag atctccagca ggaggctgtc agggcattgt cggcattccc cgaaagtttc
      361 gagaggcacg ctctcgagtc catcggcaaa gacatgttcg aatcgtggtt ttgggagcct
      421 gattgaggtg gcaaagaaaa acaatggggc gaggccgctc gcggaatcag ctattgccat
      481 ggccttagca gggatcatgt ggcacgtgtt tgcgcccatg ccgggtatcg ggccatattt
      541 caagtacatg agcgcgagcc ttattgccat tgttggttcg aggcacggaa ggcactggtc
      601 gacgatgacg gctgtgggtc tcaatgctct ggtcggcctg gcgtatggcc cgaaagagtt
      661 gttggactcc ttgctcctcg tcacgccgat cgggttgatg ttgccgacgg gcgggcctga
      721 tggggacgag gctgctgatg atagacgtga gttcggcctc ctgaatactg aaactgacaa
      781 aagcaaggcc gaggcctctt tcaggaggca cgatacctgg agtgccggca aatggtgggc
      841 gatgattctc ttaagctatg cccttctata ccccgcccgc gtatgggcgc tggcgacgtt
      901 gatgggcgct aaggtatccg atgtcgtagg gtcgatggca gcaactagct accatgtttt
      961 cgagaggttt tgggcattgg cgaatatatc agccgataca gttgtcggag cggccgtgga
     1021 ctggatgcgg attatcctgc ctgtagtcgg catgggctat ggcgcaatgc tgtatctggt
     1081 gaaccggccg gcctactttg ggggcctcgc gatcctcaaa cggacccggg tcggttgacc
     1141 gaactcagct gagtgatggc tgagatgtcg atgtcgatct catccactgc gacagtggca
     1201 tggccatctt caaactggat acctcctatg ttgatcgagc tgacggtgcc atttgccagc
     1261 gtgggtatgt cgaggcttac tatgcctttg ccaaggtacg ctacgccatc agggaggttc
     1321 gttgttgcat ttgatatgaa acgatcaacc agccctgtca tgagcggggt ggagacctga
     1381 aaggagacgg ttgggccagc agcctgaatc acacagacgt ggaatgaggg gtataggggg
     1441 ataggcgcca gcacagagag atcgactacg agttcgtcag gttcgaagtg agcgccacgt
     1501 atcctcacgt tctgcggcat ctcaatgcat ttgagggcgg cctcgagctc gtcgctgtct
     1561 atgactatct tcaatgattc gattttcatc ctatatcatc caccttgtga tggtttggtt
     1621 cgtgcccgga ttccgtccgc gacgccaaaa cttcggatgc gcgcagcgga agcgatgtaa
     1681 gaagtatgtt ttcgattatg gcgcggtagc gcacggtctc tagctcccga agagcgtatg
     1741 ctatctcggc ccggcatcgg tctagtaggt acttgagtcc tgcggatgcc tcgcctgtta
     1801 ggatcagagt gcccggaaca accatggaac atgcggccaa gccgttgaag cgcccccggg
     1861 caaggtcggt ttccaggtct tcaagacagt cggcggcgta gatgatcttg cctgcagcat
     1921 tgcctattct ccgaagcggc tcggcgttcg ggcggagccc ggcgaggtcg gccgtatatt
     1981 cgaagacctt ggcgagcccg tgcccggtcg gcgcagccag atcgtcaaag gaaacgctca
     2041 ggggattctc gacagccatc gattccagtt ttccttggcg cttttgtatc tcgtcaagag
     2101 atgatgcgtc gaagtggttt tcggcaagga ctctctcggc tcgggcgaag gcgcgcctgc
     2161 ccaaaagccg tgccggtctt gcggccctgg cgccgtctat gatcgcatcc tgacttttta
     2221 agtctaccat ggcgactgtg aaagcggaac ctattttcat gaagagttca tcgccgtcta
     2281 tgacgcaata ccgcctgggt gctcccaatg gacaaaaggc ttttgtgagc gtgggcgcgt
     2341 ccggctgctg tgccgacatg agcaggagca ggagcgtgga gtcgaaattg gcgcaggctc
     2401 gccaaagcgg gccatattgg cgcgaaagag cgaaacagag tccgcagtaa tgggctttgt
     2461 agctgcaagt ttctttagca gccaaatttc ggcatgcctg ggccccttgt tgaggcacat
     2521 tcgccgcatt ggccaggctg tgttggccgt aaggtttggt gtatccgaac atttggtccg
     2581 cctcccgctg tttcggaggt gttggctgtg actgattatc ccggcgctcg ttgcgcgggt
     2641 taaggcacgt agagtctgct tgcttccaag cgcctgttct tcgccttgga ggctgcgata
     2701 tcctgcttga accatagatt cgcgagggcg atggcggcaa tgccatgcgg gttttcgtat
     2761 gggcagagtg tggcactggt cgggagaacc catgtcgcgc gacttgagtg aacatgggag
     2821 ggggacgata caggagccta tcacgtgaac acagcgaatt cggcagtatt tatctgactt
     2881 acgcggctta agctgtgtcg agtcggccaa taacgagatg tcatctcata cttgggggga
     2941 gtcagcaagt ggatgccatg gaggctatat taaccagacg cagcattcgc aagtatactt
     3001 ccgagcctgt aaccgatgac gaagttaagc agttactgag ggcggcgatg catgccccgt
     3061 cagctggcaa ccagagaccg tgggagttca tagtgatcag ggacaaagag acgctcaatg
     3121 agattacggg gatacataag tacgcgcaga tgctcaagca agctcaggtt gccattgtcg
     3181 tgtgcggaga tgtgaaaaag cagaagtatg atggattctg ggtgcaggac tgttcagctg
     3241 ccacgcagaa catgctgctg gcggcaacgg cattgggatt gggcgcggtc tggcttggag
     3301 ttcatccagt tgtggagcgg gtggaggacc tgaggaagat cctcggcatt cccgtcaatg
     3361 tggttcccct gtcgatcgtg tcggtgggcc gacctggaga ggagagggag gcggctgata
     3421 gatatgacga gtcaaggatc cacatgcata gatggtagcg agggcgtaga ctggccgtag
     3481 accgacgagg atactaagat gcatggctgc gccggcgggg attgaggtcc ctgccggcgc
     3541 tttcctatag agctgggggc atgcccctcg gctacccaca cacgctgtcc aagtttgtgg
     3601 ttggtggtcc aatgatctca ccttcgaatg atcttgagaa ctatggcaat gatcgccagt
     3661 gcccatatgc tgacgacaac ggcccctgcg atccaactgg ttggcttttc cccgtccgtc
     3721 atggactggg ctttgagacg gagttcattc atgaggcctt cggggatgag caggagatca
     3781 tctagttgcc cgaggacggg gatgaaatcg gggatcaagt ctattgggga gaaagcgtat
     3841 cccacggtta ttgctgccac tgctttggtg tgccagggaa ctcgcctgtc tcctaacgcc
     3901 aggaatagag cgtatatgtg aacaagcatc ttcttggttt tctcacttat cttctcagcc
     3961 atctggtttt gcctctatcg ataggccggc aggtttcggc ctcgttcttg ccaggcacaa
     4021 ttgcggtcgt cgcggtcggg caagttcata atggcttatt ggcaaccact ttgtctagtc
     4081 aagacaggcg gaacggggcc gtgccagtgt cgccaagcgc cgccgagcat tgctgtgtgt
     4141 cctcaggctg cttgctgttg tcccgttagc gagtgctgcc cgtgaagact gttgcctcat
     4201 gcgtagcaag caagctctat gggcgctttg cgcgataaga tgacattcga tgttgagatc
     4261 agtcgttcac gtgatgtggc ggcttggcaa actgactggg agacctgctc caacctgtcg
     4321 gaagatgtgc gcagttagtg agaacctaag aattacagag agcgtgtgtt cgatgcgcca
     4381 attcggtgcg tttatgacta agaagcagaa cggtgcgaaa ggattaggta aaacaccgcg
     4441 aatgacatag ttaccataca atccagccac aaatcggacc tagggccatc gtcttctata
     4501 ttccaacgaa acaagcgtgt ggctttcggc gcgaggcaga agatatgaaa aaggcgttct
     4561 tcggcaactt cgcgactagc cattttgagc ccgtgagagc gccgtccacc cgccggcgca
     4621 ttacagctat cctgcttgca tacctttatt cactatgctg catgacc
//
LOCUS       DAOSAI010000184         4666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11604_length_4666_cov_3.796573, whole genome shotgun sequence.
ACCESSION   DAOSAI010000184 DAOSAI010000000
VERSION     DAOSAI010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11604_length_4666_cov_3.796573"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..546
                     /locus_tag="PLM83_06945"
     CDS             319..546
                     /locus_tag="PLM83_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK71175.1"
                     /translation="MAKFRCNLKKYRQLANLTQEELAQMVNGCRETIQQLEAGKYTLS
                     SRPAIDISRVVKAPIEDISILDRGSRAWHLS"
     gene            complement(1113..1958)
                     /locus_tag="PLM83_06950"
     CDS             complement(1113..1958)
                     /locus_tag="PLM83_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOK71176.1"
                     /translation="MKIAVVTDSASDLPNEVREAGQIHVVPIRVLFGEDQFRDQVDIT
                     PEQFFDKLNTSGVLPSTSQPSPADFINLYEILLKKHDAVISLHISEKLSGTYQSACIA
                     RDAVSPNRIEVIDTRAASMAQGFIAMEALQAAAAGSSREHVLHAVQKAMDTTRLAFTV
                     DSLEHLMRNGRIGKAASMFGSLLSVKPIIALEDGELTPMAKVRGSKRVLECVMECIRK
                     DVGNGAIKVAVIHTNTHDRAREWFTAVQGAFVCKEAYLVQCGAVIGTHVGPTGLGVAW
                     MPADE"
     gene            2314..2517
                     /locus_tag="PLM83_06955"
     CDS             2314..2517
                     /locus_tag="PLM83_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71177.1"
                     /translation="MVNIEPLQTSYSNEREVKHVLRDITLYVCAGERLSVMGPLGSGM
                     RLPAGFSAPRLGWMRFNYEPFWG"
     gene            2877..3362
                     /locus_tag="PLM83_06960"
     CDS             2877..3362
                     /locus_tag="PLM83_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOK71178.1"
                     /translation="MTAVALVLHMIESVLPPLPVPGAKIGLANVATLVCLRHMGFWPG
                     VAVSAVRSILGSMISGKFWGLGFWMSFVGATASAVAMGISLQVVASGTSLVWTSVVGG
                     VVHASCQLIIAVAAMRQPALLAGAPVLLGLSIITAVSIGLVCDNLDAPVRRLIQANKR
                     R"
     gene            3370..3726
                     /locus_tag="PLM83_06965"
     CDS             3370..3726
                     /locus_tag="PLM83_06965"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOK71179.1"
                     /translation="MRRRALPILLVLVFGVALVAFGMYEQKKSPEGSVVVVKSIGEDY
                     NRIVARQPLSSDEKVIIPVEGPLGTTVVEVVGGRAHVVSSPCPDKICVRMGWLKRVGD
                     YSACLPNRVLVEVVDE"
BASE COUNT         1053 a         1074 c         1315 g         1224 t
ORIGIN      
        1 taaacgtaag gttcaaaaaa ttagcccaag acatcaatcc aagcggctcg agccatattg
       61 aggcagtagc gcgcctggaa cgaccagatt aggcgagtct atgccctgga cgactattct
      121 ggtcctactg gcgctgtgag gagtgccctg ttctactgca ttgacaggtc aacctccaaa
      181 cgacaaaatc gaatggcttg gttttcttgg attcttgggc tttatgggtt tcctgaaatc
      241 gtatatgggc gaaccgcagt acatcttctt tgctcctccg ttcatttctt atctactact
      301 acgatcaggt agagtaagat ggccaaattc aggtgcaatc tgaagaaata cagacaactg
      361 gcgaacttga ctcaagaaga gcttgctcag atggtaaacg gctgtagaga aactatccaa
      421 cagttggaag ccgggaaata caccctatcc tcgaggccgg caatcgatat ttcaagagtt
      481 gttaaggctc ctatcgaaga tatatctatt cttgaccgag gttctcgcgc ctggcattta
      541 tcctaacaaa aagggcattt acccagtcgg ctagcgtaag ccgccccacc aacccgttcg
      601 gagacattaa cgaggttggg atagggaaaa ggacggattc aggcggcaac ggaggaaatc
      661 cgtcatgctg cagcttctat ggtatgaata caagagttct gcagcataaa tggcagactg
      721 cgtgcaagaa tggtagtctc ttatctgttg gaaccttgtt gctcatcttg ctgcctgtga
      781 ttggattaaa agctcacaga acgtgctaat cagtgggtgt atgggcgcag gctattattg
      841 tcttgcctgc gcccatacac ccactctgct gatactacat agagtgctgc gcctcatatc
      901 cgaactggcc atcgcccgcg gagaccgatc atgctggtca gactctccga gatacgtttg
      961 cttgtcctga acaagtggaa agcaggagat attgctttgc gtcgcgcctc gctcctactc
     1021 gatcaaccgg gggcttgcgg gaaccactgg attctttgct ccggaacggg tagacggttt
     1081 ttattggcat agcaatggcg gcacttggct agtcattcat cagctggcat ccacgctact
     1141 cccaacccgg ttggcccgac atgagttcct attactgctc cgcattgcac aaggtaggct
     1201 tccttgcata caaaagcgcc ctggaccgcg gtaaaccact ccctggcgcg gtcgtgtgtg
     1261 ttggtatgga ttactgccac ttttatggcg ccatttccca cgtccttgcg tatgcattcc
     1321 atcacacatt ctagaacccg cttggatccc cttactttgg ccattggagt caattcgccg
     1381 tcttccagag ctatgatggg tttgactgaa agtaaggatc caaacatcga tgcggctttc
     1441 cctattcgcc catttcgcat caggtgttct agcgagtcga ccgtaaaggc tagtctggtg
     1501 gtatccatgg ccttttgaac tgcatgaagc acgtgttcgc ggcttgagcc tgctgctgcg
     1561 gcttgcaggg cttccattgc aatgaatcct tgtgccatgg atgccgctct cgtgtcgatc
     1621 acttcgattc tgtttggact gacagcgtct cgggcaatac aagcagattg gtatgtgccg
     1681 gaaagcttct ctgagatgtg gagcgaaatc actgcgtcgt gctttttgag caagatttca
     1741 tataggttga tgaaatctgc tggagaaggc tgggatgtgg aaggaagaac ccccgatgta
     1801 ttcagcttgt caaagaattg ctcgggtgta atatctacct gatcccggaa ttgatcctcg
     1861 ccgaaaagga cgcgaatcgg tacaacatga atctgtccag cttctcgaac ttcatttggc
     1921 aggtctgatg cagagtcagt caccacggct atcttcattt cgtcaccctc aaacggtatt
     1981 ttcggatgct gactgtatag ggcgccattc gaaaagcggg gaaatcgggc tgtaggggcc
     2041 ggaggcgctg ttaggctatt gaagctgtcg gcatccaaaa agtattttgt acatcaaaca
     2101 atatgataaa caaatcatat aatgccgttt cgtgtttgtc aattagtttg gaataccgga
     2161 gcttgagctg ctgtagcgtt gggaccagca gaacagtcaa ttccaaggtg taccgctctc
     2221 agacgactgg gggaggaccg cctgtcccgt gcaggcttgt agtcacttgc tgacatactg
     2281 acgatggcca ttacggacaa aggggtgctt tgcgtggtca acattgagcc tctacagacg
     2341 agttacagca atgaacgcga ggttaaacac gtgttgcgcg acattactct gtacgtatgc
     2401 gccggggagc gtctctccgt gatgggcccg ctgggctcgg gcatgcgatt gccagcaggc
     2461 ttctctgctc cgaggcttgg gtggatgcgg ttcaactacg agcctttttg ggggtgagac
     2521 tcggtcggcg gtgctttttc tgctgtctgc ttttccagta ggctgctctt ttggcgtcta
     2581 gcctgcagca aaactgccag actcaaccag gtagaattcc ttggaagtga gtggcagggg
     2641 gaacaagtga aagacctgga tgtcatgtct gtgccatgcc ctagacggag gttagcgcta
     2701 ggagtttgcc cgtcgagtgt catcgcaggc aaagcgaata tggtatataa tttggattgt
     2761 ggcttagaaa tgcttgtagc agcgtttttg ttggctttga tgttgaactc gatgccaatg
     2821 gcggtgattt gcagttgaag tcaggcaatc tgcagcgaca agtgcagctg gcaattttga
     2881 cagctgtagc tcttgtcctt cacatgatag agtccgtgtt gcccccgctg ccagtgccgg
     2941 gagcgaagat aggccttgcg aatgttgcga cattagtatg cctgcgccat atgggatttt
     3001 ggcctggcgt tgcagttagt gcagttcgta gcatacttgg aagcatgata tccggcaagt
     3061 tctggggttt ggggttctgg atgagctttg tgggcgcaac tgcgagtgcc gtggccatgg
     3121 gcattagcct tcaagttgtt gccagtggaa catcgctcgt ttggactagc gtcgtcggag
     3181 gagtagtcca tgccagttgc cagttgatca ttgctgtagc cgctatgaga cagcctgcgc
     3241 tccttgctgg tgctccggtt ttgctagggc tttcgataat cactgccgtt tcgatcggct
     3301 tggtatgcga taatcttgat gcgcctgtcc ggcgtctgat acaggcaaac aagagaaggt
     3361 gatgcataca tgagacgcag ggcattgcct atcctgcttg ttttggtgtt cggtgtagcg
     3421 ctagtggctt tcggaatgta tgagcagaaa aaatcccccg aaggctcggt ggtggttgta
     3481 aagtccatag gcgaagatta caacaggata gtcgcccgac agcctctatc atcggatgag
     3541 aaagtgatta tcccggttga aggaccgttg ggcactaccg tagtagaggt tgttggagga
     3601 agggctcatg tggtctcgtc accctgccct gacaagatat gtgtgcgaat gggctggctg
     3661 aaacgtgtag gcgactacag cgcatgcctt cccaataggg ttcttgttga agtagttgat
     3721 gagtgagctt ttgcatgggg gattgcgagg cttgtaatga ttgagggact gggacagagg
     3781 cagcaaggcg ggagagctcg aactgggttg atcccgcctt gctatttcta gtggtcagga
     3841 ccctctgggc aataagtggc tcttcgaaca ccaagtgtaa ataaatggct gcgattgcgc
     3901 gtggctcagg cacctcgatg gctaccatta cagtatctac tgccacgtgc cgtttaggaa
     3961 tgtagcgaag tatgtagatg atctgccagt tgccttgttg ggcagtgccg actgtgtagt
     4021 ggacagatgg gtgaggcttg gggagaagga gcctttgttt tgctcctagg ctgcggtatg
     4081 ccccggacga atatgaaaca ttgagctatc atggctgtcg accacgagca tgccatgtca
     4141 caagttggag gtgaatggca cggtcctgta gccaaagccg tatgcgggaa cgaagcatgg
     4201 gggtgccggt ctcctgtggg cctcgcactt tagcgcgccc gatatcgcgg ttacgatcgc
     4261 cggccagaaa gcctcttgac ttttgctagt acgtccgttt gacgcttgga ttgggttgca
     4321 agctaactct gtgaatgtga atcctgtcac tatggacgat gattttgtga agtgataagc
     4381 ttggatagaa tcaagccctg cttcagaaag ggaaatgaaa aaggcggcat tggttcggga
     4441 tgtaattcgt acaagccttg ttccaccggt tgcttgatct tcaagcggtt gggctgtcta
     4501 gaagccgatt gtatagtcgg gtacatcaca aatccaaggg gctttcggtt atcgctaatg
     4561 gcaagggtaa ttgctccggc agcttagatc tgcactacca tcgcctaagc ggtctgtttg
     4621 gcaatcgaaa agttcatagt tcggcagtca aaaagtgcat aggcta
//
LOCUS       DAOSAI010000185         4661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11618_length_4661_cov_3.902301, whole genome shotgun sequence.
ACCESSION   DAOSAI010000185 DAOSAI010000000
VERSION     DAOSAI010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11618_length_4661_cov_3.902301"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            669..1160
                     /locus_tag="PLM83_06970"
     CDS             669..1160
                     /locus_tag="PLM83_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71180.1"
                     /translation="MKRFLALLVACVVFTAFSGLALSSNSANQTVTFEIQAINEISVS
                     GSPAKLTISTATAGGNPDNVTDSSTTYSITTNETGKKITGKIDTNMPSNTQLEVNLTA
                     PTVGTSSGNVALSTTAADLVIGISKVADSDLQITYILSATVAAGVVNETSRNVTLTLT
                     DAG"
     gene            1265..2095
                     /locus_tag="PLM83_06975"
     CDS             1265..2095
                     /locus_tag="PLM83_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71181.1"
                     /translation="MKKISVNVVALWVIGAMLAVMLCSTVAFANVRVLGKLTHEMTVL
                     SGQKVHGSIVLANSGSIPIEVDIELRDYTFSADGTDDYLEPGTNARSNARWLVVAPAR
                     LVVPARQLGIVYYTVSVPNDPTLKGTYWSILLIKPKTDAQMVMFQSKGGFMLGIQTNL
                     EYGVQLVTNIGSTGSKSLRIMDRKVVRDQDGYYSLWFALENSGERWIRPEIRVEVYDM
                     SGALVGKYSGDRLRLYPGTSVASRIALGILPRGRYKAVLIIDDGGSNVWAAQYIWTIS
                     "
     gene            2111..>4661
                     /locus_tag="PLM83_06980"
     CDS             2111..>4661
                     /locus_tag="PLM83_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK71182.1"
                     /translation="MNLHVSPWVMFLVTIVFCFCQPQVSKASCSETLYGCSIIIQAPS
                     YIEAMPGQTLTLGFVVTNVSEVQLNLEESVFLPPEWRLLIDIPGFSLDPGGSDLRLVV
                     VSIPQDCPWGDYVVSYVVSDSSNSNSCGCMYVSVSVSALPSLAILTIEAPPYVLAGEE
                     YSVVYNLMNLGNLRADIEVRAGVNEAVPAVVSPSELTLDPGESKQVIAEVITDGKRPQ
                     VYTSVLRLTVTATYPTGHVSDHECISRIEVIPLGGSEVREYNLGRFEFASIASHDPKR
                     SALTLELSGSGPLTEDKGVNLDFILRTKQIATAVDQISDDQWQFKLTGEHGQFRVGDG
                     YFALSALTEAGVRGCGVEGSMEVVIGRIGGYWIIAQASGAEERALYLNADLKNNEGAK
                     VTYLRKTIPLSGGVVNDRICSVESRAMCYGDIELGGGFGLSLRERQGDEDELGTSCHL
                     QVSGSAQNLQYSVGILAADHNYCGPVRDAAQFNLFLATTPVPRLSVWGLIYGQHTGAG
                     DLSRTVAAGLAWNINEKSSVALSSRSTSKCEAEGMLEKFGTFTNLSFAHTEEKTRLDG
                     YLQLIREQETCGKTNEAKKLGISLSCVLSPNKVYKAYYRTDMGQISKRFIGASASYSL
                     PNSQKILADLSLERAATGKASIYRIDIGYSACFDIPFSIKRDKEVVKGVVRRSEDHGY
                     LGVPNVLVRMDGMAVFTGSDGIFVLSGVAPGVHYIDIDRSSIPSNLIPAVQLPLRVDV
                     LAGTEVELVIDLTQSGSIAGTVTRLGPEEEIVLLPGGHEKVIGERVGIYGVIIELRGE
                     LETLRSKTGPQGEFQFTALRPGTWVISIPQFGDDVERIVVTIEPGEEAIVNI"
BASE COUNT         1189 a         1010 c         1281 g         1181 t
ORIGIN      
        1 ggtttgccag gagccgtgta cggggcccgt acgcacggtt ctgtgagagg gatgatgccc
       61 acctggcgcc accccactcg attcctcctt ccatggcata tgcgaatgtc acgaactcgg
      121 aatcatcgca tgcgccttcg aagtatccca tatcatgagc tcgggcgaga cgccgaagtc
      181 ccttatgggt ttgagacaac gcccaaactt attgtcgttg agcaggtcag aagatatgcc
      241 gaagagatca atggtaaata tatgatccgg ccaatcctga attttgtaca acggtcttgg
      301 atagttgatc ctattcatga caagaatcct gcaagcttcg cgttgtaaaa aacctgatgg
      361 gtgtgggttg aaggtccgat tatgctgccg tttggggttt ctgagcgtgc gtccatcagc
      421 ctccacatct aacgtgcttt ctcaggacat tctcatcgct atttgaagat cgagcttttt
      481 ctactgcgaa aaatgagctt agagtcatat tatcgtatga cacacatagg cataatactg
      541 cagaaggcag tgccgggatg catgttgatc ccatacgcac cgttccagcc tccagtagct
      601 gttcatatcg tgtaatggct cacttgacaa ggcgagtcag aatagcctac tggaaaaggg
      661 ggataatcat gaagcgtttc ttggcgctcc ttgtggcgtg tgtggttttt acggccttct
      721 cgggattggc gcttagctcg aattcagcga accaaacagt gacttttgaa atacaagcga
      781 ttaacgagat tagtgtcagt gggagccctg ccaaattgac catcagtacg gctaccgctg
      841 gaggcaatcc cgataacgta actgacagct caacgactta cagtattaca actaatgaaa
      901 cagggaagaa gatcactggc aagatagata ccaacatgcc ttcgaacact cagcttgagg
      961 tgaatctcac tgctccaaca gtcggaacga gctccggaaa tgtagcctta tccacaacag
     1021 ccgccgacct agtaattggg atcagtaaag tggcggatag cgacctccag ataacatata
     1081 tactgtctgc aactgttgcc gcaggcgtag tcaatgagac ctcgcgtaat gtcactttga
     1141 ctcttaccga cgcgggctag cccttcattc tgatgatgcg agggagcttt cgccgttgac
     1201 cgcggctccc tcgctgcacg tagattagtc ctgattgagc caatttgcaa aggagagaat
     1261 atacgtgaag aagatatcgg tgaatgtggt tgccttatgg gtaatcggag ctatgcttgc
     1321 cgtgatgttg tgctccactg ttgccttcgc caatgtcagg gtcttgggga agcttacgca
     1381 tgaaatgacg gtgttatctg gtcagaaggt tcatggctcg atagtgttgg ccaattcggg
     1441 atcaatcccg atcgaggtag acatcgagct gcgggattac acattttcag ctgatggaac
     1501 agatgattac ttggaaccgg gaacaaatgc acgttcgaat gcgagatggc ttgttgttgc
     1561 tcctgctaga cttgtcgttc cagccagaca attgggcata gtttactaca ccgtatcggt
     1621 tcctaatgat ccaacactga aaggaacgta ttggtcaata cttttgatca agccaaaaac
     1681 agatgctcag atggtcatgt tccagtctaa aggtggtttc atgcttggca ttcagacgaa
     1741 cctcgaatat ggcgtacagt tggttaccaa cataggcagc acggggagca aaagcttgag
     1801 aattatggat cgaaaagtgg taagagatca agacggctac tattcgctat ggtttgcact
     1861 ggaaaacagt ggcgaacgct ggattcgacc tgagatacgg gtcgaggtgt acgatatgtc
     1921 aggcgctctg gtggggaaat actcaggcga taggttaaga ctttatccgg gaacatcggt
     1981 tgcgtcgcgg attgctctgg ggattctccc tagaggcaga tacaaagctg tcttgattat
     2041 agacgacggg ggcagtaatg tctgggcagc tcaatacatt tggaccatat cttagggggt
     2101 gaagacaggt atgaacctgc acgttagccc atgggtgatg ttccttgtga ccatagtttt
     2161 ctgcttttgc cagccgcaag tttccaaagc tagctgcagt gagacgttgt atggctgtag
     2221 cataatcata caagctccgt cctacataga agcgatgcca ggacagaccc ttacccttgg
     2281 ttttgtggtg acgaatgtat cagaagtgca attgaatttg gaagaatccg tttttcttcc
     2341 gcctgagtgg agacttctca ttgacatacc cggcttttcg ctggatcctg gcggctcgga
     2401 tttacggctt gtcgtcgtct cgatcccaca ggattgcccg tggggtgact atgtcgtgag
     2461 ttatgtagta tccgactctt ccaattctaa ttcgtgcggg tgcatgtatg tctctgtgag
     2521 tgtttcagct ttaccatcgt tggccatatt gacgatagaa gctccaccgt atgtgctcgc
     2581 cggagaagag tattctgttg tatacaatct gatgaacctt ggcaacctcc gagctgacat
     2641 cgaagttcgg gcaggggtga acgaggctgt tcctgcagtg gtatcgccca gcgagttgac
     2701 tctcgatccg ggagagtcga agcaggtgat tgcagaggtt attacggatg gaaaaaggcc
     2761 acaagtttat acttcagttc tgcgtttgac agtaacggca acatatccga cagggcatgt
     2821 ctctgaccac gaatgtatca gtaggattga ggtgatcccg ttggggggat cagaggtcag
     2881 ggaatacaat ttgggccgct ttgaattcgc gtccattgcg tcacatgacc ccaagagatc
     2941 agcgctcaca cttgagctta gcgggtctgg tcctttgaca gaggataaag gggtcaacct
     3001 cgatttcatt ttgaggacta agcagattgc aactgctgtg gatcagatat cggatgatca
     3061 atggcagttc aagcttacag gcgaacatgg acagtttagg gtgggtgatg ggtattttgc
     3121 gctttcagct ttgacagagg ccggagtgcg cggctgcggc gtggaaggct caatggaggt
     3181 ggtgattggg agaatagggg gatattggat cattgcgcaa gcgtccggag ctgaagaaag
     3241 ggctctgtac cttaatgcag acctgaagaa taatgaagga gcaaaggtga catatctgcg
     3301 caaaacgata ccgttaagcg ggggtgtagt aaacgacagg atatgtagtg ttgaaagccg
     3361 tgccatgtgc tacggcgata tcgaacttgg gggcgggttt ggtctcagcc ttagagaacg
     3421 gcagggggat gaggacgaat tgggaacatc ttgtcatttg caagtatcgg ggagcgcaca
     3481 aaacttgcag tattctgttg ggatcctggc tgcggaccac aactattgtg gaccagtgcg
     3541 agatgctgct cagttcaatc tctttttggc aaccactcca gtcccccgtt tgagcgtgtg
     3601 ggggttgatc tacggtcaac atactggagc cggcgatttg agtcgaacgg ttgcggccgg
     3661 gttagcttgg aatataaacg aaaaatcatc ggtagcattg tcttccagga gcacatcgaa
     3721 atgtgaagcc gaaggtatgc tcgaaaaatt cggcacattt acgaatctga gctttgccca
     3781 cactgaagaa aaaacaaggc tggatggata tttacaatta ataagggaac aagaaacctg
     3841 cgggaagacc aatgaagcca agaaattagg gatttcgctt tcttgcgtcc tttctccaaa
     3901 caaggtatac aaggcatact accgcacaga catggggcaa atctcaaagc gcttcattgg
     3961 cgcaagtgca agctactcac tcccaaactc ccaaaaaata cttgcagatt tgtcccttga
     4021 acgtgctgca acaggtaagg cgtctatcta tcgtatcgac attggctatt cggcttgttt
     4081 tgacattcca ttctcaatta agagagacaa agaggtagtc aaaggagtcg tccggagatc
     4141 tgaggatcat ggttatcttg gggttccgaa tgtgctggtc agaatggatg gaatggctgt
     4201 tttcacaggt tcagatggaa tatttgtatt gagcggcgta gcgccgggcg tacactatat
     4261 agatatagac cgaagctcaa taccgtccaa cctaattccg gcggtccagt tgccgttacg
     4321 cgttgacgtg ttagcaggaa cagaggtaga gttggttatc gacttgacac agagcggatc
     4381 tattgcagga acagtgactc gcctcggccc tgaagaagag attgtgcttc tccctggcgg
     4441 gcatgagaag gttattggag aacgagtcgg gatctatggc gtgataatcg agcttcgcgg
     4501 cgagctcgaa acgctgagga gcaagactgg gccgcaagga gaattccaat ttacggcatt
     4561 gaggcctgga acatgggtta tttcaatccc tcagtttggg gacgatgtcg aacgtatcgt
     4621 tgtaacaatt gaaccgggag aagaggccat agtcaatatc g
//
LOCUS       DAOSAI010000186         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11639_length_4656_cov_3.947403, whole genome shotgun sequence.
ACCESSION   DAOSAI010000186 DAOSAI010000000
VERSION     DAOSAI010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11639_length_4656_cov_3.947403"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PLM83_06985"
     CDS             <1..1039
                     /locus_tag="PLM83_06985"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOK71183.1"
                     /translation="NHGTKQAQSARRLTADFSVALTGPHVENRLFELWSIMDFLNPGY
                     LGSAAGFRQHFATPIERYGDAAASARLRKLTAPFILRRVKTDPLIIQDLPEKHETKVY
                     CNLTKEQATLYEAVVRDMIATIEEASGMERRGAILAALLKLKQVCNHPAQFLHDNSRL
                     EGRSGKLSRLEEMLDEIVEEGDRALIFTQFSEMGTLLKHYLSKALRQEVLFLHGGVPQ
                     KERDRMIERFQNDPNGPPIFILSLKAGGLGLNLTQASYVFHFDRWWNPAVEDQATDRA
                     FRIGQKKNVNVYKFLCGGTVEERIDQMIDEKRALADSIIGTGEEWITEMSTDELRNIF
                     TLRDESIIDDD"
     gene            1091..2002
                     /locus_tag="PLM83_06990"
     CDS             1091..2002
                     /locus_tag="PLM83_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOK71184.1"
                     /translation="MARKNEFGGSWWAKRWTFALESFGWANRLQRGRSYARAGNVLSV
                     DVSPGMAEAKVQGSRSRPYKVQIRMKPLNESDWDRVTTAMAESAAFAASLLAGEMPEN
                     IEEAFAQCDAALFPVSEEDLATSCTCPDWANPCKHIAAVHYVLAQEFDRDPFLLFLLR
                     GMRKEDLLISLRRKRSQAACQEASEPENTTRCTDAAAALAEDLCPSPEEFWNGDVEHL
                     DHVAISIAPPSIDAVAIKHLGPLPLRDQGNTNLSRTFIQTMDHYYKAVTKQAITWAYG
                     DDQEGSTAFDCNSHKDNGTVHKKSLQR"
     gene            complement(2464..4530)
                     /locus_tag="PLM83_06995"
     CDS             complement(2464..4530)
                     /locus_tag="PLM83_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOK71185.1"
                     /translation="MSSGMSATGLRRWIRESKLWILPIYLFLIIFVIFPLVRLFVDSF
                     TTADIPLSALNEVVLLVDMLEEKVNAGEIEDALAASHDIALQLDRSVDVIGHMIDALG
                     RATFSQDELIAKWQKAQEDVAKEAAVMAELQDDPVAALAESRKATALIDEVVSLPRRA
                     FSVEFFLDVFRDELYMKALGNSLLLGLATVVTTSVIGFAVAYLLVRYDFPGRKVYNFL
                     TTVPIVMPPLVGVLGFIFILGRGGMVNELFYQLVDVLESSWPAMSNWLFDRLPFNFIY
                     GWHGLLLVETFHLFPLITLNVLDSLSKIDASLEEAAESVGSVGFKRLFDVTIPLTIPG
                     FVTGALLVFIGAFADFAAPMVVGLTNFIAPLAYMDIQQFTDRHLFKMGITVGAVMVVI
                     SVVLLLIAKKYISLKDYTTLSYRAVERKPLEGKKQAWVHVFLILLLVISYTPYLGVAL
                     ASFSKSWSMSPLPTAWTLAHYEKILLYAPGYIINTFQFCILAIIMCIVIGLPVAWIMA
                     RTDTWSSGALDILTTLVLALPGTALGIAYIRAFNTPILGSVPLANIWIIIPIVLAVRR
                     LPYTVRSSYGSLLVVHRSMEEAAQSVGAGGFKTFKDITLPLVWKGVLAGALFSFITSI
                     QESAATLLLAIPGWETMTVGIFTFYTGGTHGDAAALGFILIVVGVITLFIMDRLSSAT
                     AGGLFG"
BASE COUNT         1210 a         1306 c         1176 g          964 t
ORIGIN      
        1 gaaccacggc acaaagcagg ctcagagcgc ccgcaggctg accgcggatt tcagtgtggc
       61 cttgaccggg ccccacgtag aaaaccgtct ctttgaactg tggtctatca tggattttct
      121 caaccccggt taccttggct cagccgcagg cttcagacaa cactttgcaa cgccgataga
      181 gcgctatggc gatgccgcag catccgcccg gctccgcaag ctgactgccc ccttcatctt
      241 gcgtcgagtc aaaactgacc cgctaataat acaggactta ccggaaaagc atgagaccaa
      301 agtctattgt aatctcacaa aggagcaggc aaccctgtat gaagcagttg tccgcgacat
      361 gatcgccacc atcgaagaag cttcaggcat ggagcggaga ggagctattc ttgcggctct
      421 tctcaaactg aaacaggtct gcaatcatcc tgcgcaattc ctgcatgaca acagcaggct
      481 ggaaggacgg tcagggaaac tctcgcgcct tgaggaaatg ctcgatgaga tagtggaaga
      541 aggggacaga gcccttatct tcacgcagtt ttccgaaatg ggaacgctcc tcaagcacta
      601 cctttccaaa gcactcaggc aagaagtgtt gttccttcac ggtggcgtgc cgcaaaagga
      661 aagagacaga atgatagagc ggttccaaaa cgatcccaac gggccgccca tcttcattct
      721 ctccctcaaa gccggaggcc tggggctcaa tctgacgcag gcaagttatg tatttcattt
      781 tgaccgttgg tggaaccccg ccgtggagga ccaggcaacg gacagggcct ttcgcatagg
      841 ccaaaagaaa aacgtcaatg tctacaaatt cctctgcgga ggcaccgttg aagaacgaat
      901 tgaccagatg atagatgaga aaagagcatt ggcggacagc ataattggaa cgggcgaaga
      961 gtggatcacg gaaatgtcca ccgatgaatt gcgcaacata ttcaccctta gagacgaaag
     1021 catcatcgac gacgactagc gtcaatagct cggcggataa cccgagcggc gcatgggagg
     1081 ctattggttc atggcgcgga aaaacgaatt cggcggcagc tggtgggcga aaaggtggac
     1141 ctttgccctg gagagtttcg gctgggcaaa ccgactgcaa agaggcaggt catatgcgcg
     1201 ggcgggtaat gttctttccg tagatgtcag ccccggcatg gcggaagcca aggtgcaggg
     1261 ttcccgctct cggccataca aggttcaaat aaggatgaag cccctaaacg aatcggactg
     1321 ggatcgcgtg acaaccgcca tggccgaaag cgcggcgttt gccgccagcc tcttggccgg
     1381 cgaaatgcct gagaacatcg aagaagcttt cgcgcaatgc gatgcggccc tcttccccgt
     1441 ctcagaggaa gacttggcaa catcgtgcac ttgccctgat tgggccaacc cctgcaagca
     1501 cattgcagcc gtccactatg tcttagcgca ggagttcgac cgcgaccctt ttctcctctt
     1561 cctcttgagg ggaatgagaa aggaagacct gctaatttcg ttgaggcgca agcgctctca
     1621 agcagcatgc caggaagcat ccgagcctga gaacacaacg cgctgcacag atgctgcagc
     1681 cgcgctggcc gaagatctct gcccgtcccc cgaggaattc tggaacggcg atgttgagca
     1741 cttagaccat gttgctatct ccattgcgcc tccctctatc gacgccgttg ccatcaagca
     1801 tctagggccg cttcctttgc gggatcaagg caacaccaac ttgagccgga cattcatcca
     1861 gaccatggac cattactaca aggcagtgac aaaacaggcc atcacatggg cttacggcga
     1921 tgatcaagag ggctccaccg cgttcgactg taattcacat aaggacaacg gcacagtcca
     1981 caagaaatcc ctgcaacgct gatcatgaat gcgcctggct tcaaactcgc gccggtctcc
     2041 cgcaaaaccg aagcctcttt cgctcactcg ttacaccact gcttaaaaag tagttgcccg
     2101 acgcagagaa tgctgcatcc cgcctcctcg ctgggctcca acgcgcataa tgactgagga
     2161 ttcaaacccg ggcaagtcgc agcagatgtt tgagccatct ttgacagcct gggtttgaat
     2221 cctcaggcgg taagaagtcc atagccgcac ggagaattct gtctctttct gctgcatagc
     2281 gcagagcctc ttatgttgcc ctacgacgtg caaaagaaaa gccgatcaca acattgcgca
     2341 acgcgctccg ccacgatcgg ctatttattc atccgactgc caggcacgat actgccgggc
     2401 cagcccagac gttttcctga atcacctctc catgaggtcc agtcattcca ctgattggct
     2461 gcgctatccg aacaacccgc ctgctgtggc gctgctcaac ctgtccatta tgaacaacgt
     2521 gatcacgccc acaacaataa ggatgaatcc tagagcagcc gcatctccgt gggtccctcc
     2581 ggtgtagaag gtgaatatgc cgacggtcat cgtctcccaa cccggtatgg ccagaagcag
     2641 tgttgccgcg ctctcctgta tagatgttat gaaggagaac aacgcccccg ccagcacccc
     2701 tttccaaacc aacggaagag tgatgtcctt gaatgtcttg aatcctcccg cgcccacaga
     2761 ttgggcagcc tcctccatcg aacgatgcac gaccaagagg gagccgtagg aggatcggac
     2821 cgtgtatggg agccgtctca cagccaacac gattgggatt atgatccata tgttggccaa
     2881 tggcacgctc ccgagtattg gggtgttgaa agctctgatg tatgctatgc ccaaagcagt
     2941 gccgggcaaa gccaacacca acgtggtcag gatatctaac gctccactgc tccacgtatc
     3001 tgttctggcc atgatccatg ctacgggcaa tccgatcact atgcacatga ttatggccaa
     3061 tatgcagaat tggaaggtat tgatgatata ccctggtgca tacagcaaga tcttctcata
     3121 atgtgcaagc gtccaagcag tgggaagcgg cgacatcgac cagctctttg agaaagacgc
     3181 aagcgcaaca cccaagtacg gggtgtagct gataacaaga agcaggatca ggaaaacatg
     3241 gacccacgcc tgcttcttgc cttcgagtgg tttcctttcc accgccctgt atgaaagagt
     3301 ggtgtagtcc ttgagggata tgtatttctt ggctatcagc aataacacta cagagatcac
     3361 caccattacc gcgcccacgg taatgcccat cttgaacaag tgtctgtcgg tgaactgctg
     3421 aatatccatg taggccaggg gagcgatgaa gttggtcaag cccaccacca ttggagctgc
     3481 gaagtccgca aatgccccga tgaaaacgag caatgccccg gtgacaaatc ccggtatagt
     3541 cagggggata gtgacgtcga acagacgctt gaatcccaca gatccaacgc tttcagccgc
     3601 ttcctccaag gatgcgtcta tcttgctgag cgagtccagg acattcaggg taataagcgg
     3661 gaacaagtga aatgtctcaa ccaacaagag gccatgccag ccatagatga agttgaacgg
     3721 caaacggtcg aaaagccagt tagacatggc aggccatgag ctttccagga cgtccaccaa
     3781 ctgataaaac agctcgttaa ccatgccgcc tctgcccagt atgaagatga accccagcac
     3841 tcccactaga ggaggcataa ctatgggtac agtggtaagg aaattgtaga ccttcctgcc
     3901 gggaaaatcg tatcttacga gcaagtatgc cacagcaaat cctatcactg aagttgtgac
     3961 aactgtggca aggcctagca acaaggaatt tcctagagcc ttcatgtata actcgtctct
     4021 gaaaacatcg agaaagaatt ctaccgagaa agcccgtcgt ggaagactta caacctcatc
     4081 tatgagtgcc gtagccttgc gtgactctgc aagagcggcc actggatcgt cttggagctc
     4141 cgccatcacc gccgcttcct tcgccacatc ctcctgggct ttctgccatt tggctatgag
     4201 ctcgtcttga gagaacgtcg ccctgccaag cgcgtcgatc atgtgtccaa ttacgtccac
     4261 agacctgtca agctgaagcg cgatatcatg tgacgctgcc agagcgtcct ctatttcccc
     4321 tgcgttcact ttctcctcaa gcatatcgac gaggaggacc acctcgttca gcgccgaaag
     4381 cggtatgtcg gccgttgtaa acgaatcgac aaagagcctt accaggggaa atatgacaaa
     4441 tattatcaag aagagataga tgggcagtat ccatagtttg gactctctga tccaccttct
     4501 caaacccgtt gcgctcattc ctgaagacat aatacaatca cctgcccgtc catggtcgga
     4561 atcagcgtat caatccgcta tgacaagcgc agcagatttc ggaaagcaga aatacacgtc
     4621 actaccccac gacaactcct catgatgcaa cgggtt
//
LOCUS       DAOSAI010000187         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11640_length_4656_cov_3.430341, whole genome shotgun sequence.
ACCESSION   DAOSAI010000187 DAOSAI010000000
VERSION     DAOSAI010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11640_length_4656_cov_3.430341"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1987
                     /locus_tag="PLM83_07000"
     CDS             320..1987
                     /locus_tag="PLM83_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOK71186.1"
                     /translation="MKTNRPTSVAAPSNGKTVSFAVLTVACIFIFFGSATAGAESGLH
                     APYLIIHVDAVPSRLFFEEYDAGHLPNIKAVFDDGKLVRHAVSPFFAGTEMLYPRLKT
                     GAGPDQPLPITWEMYDRNSGRHISRIETQVILFSHLPTYSLTQIIHGIPGMDYLSGWA
                     LLNTPRLLDEYDIVEFFWFITDSYGHVFGEEAQRESLLILDEFLGILMKQLGSKPVNL
                     VIYADHGMSMFERTYDAESAMAETAGDNEQFVYYPNLYLKDRTLAATTAEQLANRDEI
                     DFAFYRADEFLIIGIHKEGQVMFRSEGNLITYSCEGPDPFGYADLGCLGRPLSDQDWL
                     EMTCTSRYPAVPVQIVRYMDNALSGDIVAVLNPPKGLRTTVGFCGCHTGFVDTDCVVG
                     VLMKGPDIDPSQIPDTFWLYEMYSQVLKIDPQNPTKADRAKNFFMVSDGGIDLQLSAF
                     RNNTALSIGPQEWTFIGETTIARSFITQVRAGLGLASDKSLAGGLTPVASLRLDIYFG
                     PLTYDHRNTIALTDGCRKSTSTLRFEAKNGVVLEWEYPNRIKLGVSW"
     gene            2565..2897
                     /locus_tag="PLM83_07005"
     CDS             2565..2897
                     /locus_tag="PLM83_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOK71187.1"
                     /translation="MRLHGPDEPVYTIGVAARLLGVSPQTLRMIEREGLLEPARTEAN
                     TRLYSENDLLLLQRICFLVREEGVNLAGVKMILEIESKQGNTTIRYREVTQSVPREAK
                     PGEGNGNS"
     gene            2954..>4656
                     /gene="dnaK"
                     /locus_tag="PLM83_07010"
     CDS             2954..>4656
                     /gene="dnaK"
                     /locus_tag="PLM83_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOK71188.1"
                     /translation="MGKVVGIDLGTTNSVIAVMEGGNPTVIVNSEGARLTPSVIGFSK
                     SGERLVGQLARRQAVMNPENTVYSIKRFMGRRFDEVEEERNRVPYHVKASSNYGIKID
                     VPATGRDYSPEEISAMLLQKLKTDAEKFLGEPITKAVITVPAYFNDSQRQATKDAGRI
                     AGLEVLRIINEPTAAALAYGLDKKKNETILVWDLGGGTFDVSILEVGEGVFEVKSTAG
                     DTHLGGDDYDQRLVEYIADEFQRDQGIDLRKDRQALQRLIEAAEKAKIELSQVYETNI
                     SLPFITADQTGPKHLETKITRAKFEELTSDLTERCTRPFKQALSDAKLSLDDIDEVIL
                     VGGATRMPVIQELVKRLTGKEPHRGVNPDEVVAVGAAIQAGVLAGEVKDVVLLDVTPL
                     SLGIETLGGVSTKLIERNTTIPTRKSQIFTTAEDGQTSVEIHVLQGEREFARDNRTLG
                     RFTLQGIPPAPRGVPQIEVSFDIDANGIVNVAAKDLGTGKEQRITITASTQLSKDEVE
                     NLVREAERAAAEDKKRREEVELKNSADSLVYVTEKTLREMGDKVPAETRSKVEAELEE
                     L"
BASE COUNT         1234 a         1211 c         1197 g         1014 t
ORIGIN      
        1 agtatcatgc aacacttccg aactcctcac agtctacggc atgcggaact tctgcatatt
       61 ccccggcagc ttttccaatg ctctactgtt tcctgtcatt cgcccactgc cgccacgttg
      121 atctgaaacg gcatggcttt gattcataca tcgactacgc agattgccat catgcaaatc
      181 gggggtaccc gaacaatctc cactgattat tggttcaggc tgtactcgca tttgtctcgt
      241 acaagcgtca tataagagat atagtataaa atagagcata atgggatatt ctcggtccca
      301 cgctgggagg atgacttagt tgaaaacgaa tcgcccgaca tcagttgcag ctccttcaaa
      361 cggaaagacg gtctcctttg ctgttctcac cgtcgcctgc atattcatat tcttcggcag
      421 tgcaacagcg ggagccgaat ccggattgca tgcaccttat ctcataatcc acgtcgatgc
      481 cgtaccgtct agactgtttt tcgaagaata cgatgcgggc catctgccga acatcaaggc
      541 agttttcgac gacggcaaat tggtccgcca tgcggtctcg ccctttttcg caggcacaga
      601 aatgctttat ccaaggctta aaaccggagc aggcccagat caaccccttc caatcacgtg
      661 ggaaatgtac gaccgcaact cgggacggca catatcgcgc attgaaaccc aagtaatact
      721 cttttcccac ttgcccacat actcgctcac gcagatcatc catggcatcc ccggaatgga
      781 ctatttgtct ggctgggctc tgcttaacac tcctcggctg ctcgacgagt atgacatcgt
      841 cgagttcttt tggttcataa ccgactctta tggccatgtc ttcggtgaag aagcccaaag
      901 agaaagtctg ctgatactcg acgagtttct aggaattcta atgaagcaac tgggttcgaa
      961 gcctgttaac ctggttatct atgctgacca cggcatgtcg atgttcgaac ggacatatga
     1021 tgccgaaagc gccatggctg aaactgcggg tgacaatgag caattcgtgt actaccctaa
     1081 cctgtatctg aaggacagaa cacttgccgc gactacggca gagcaactcg ccaatcggga
     1141 cgaaatcgac tttgccttct accgcgcaga tgagttcctt ataataggca ttcacaaaga
     1201 gggccaggtg atgttcagat ctgaaggcaa cctgataacg tattcctgcg aaggaccaga
     1261 tcctttcgga tacgccgatt taggctgttt ggggcggcct ctgtccgatc aagattggct
     1321 ggaaatgaca tgcacctcgc gctacccagc cgtgccagtt cagatagtca gatacatgga
     1381 caatgccctt tccggcgaca tcgtagccgt tttgaatcca ccgaaggggc tcaggaccac
     1441 ggtgggattt tgcggatgtc atacagggtt tgtagatacc gattgcgtag ttggcgttct
     1501 gatgaaaggc cccgatattg acccttcgca aattcccgac accttctggc tgtatgaaat
     1561 gtacagccaa gtattgaaga tagatccaca gaacccgaca aaggccgaca gagctaaaaa
     1621 cttcttcatg gtttcagatg gcggaattga tctgcaactc tccgcgttcc gaaataacac
     1681 cgcgttgtca atcggtccac aggaatggac ctttatcggc gagacaacca ttgcgcgatc
     1741 ctttatcaca caagtgcggg caggactcgg ccttgcttct gataaaagcc ttgctggagg
     1801 gctaacaccg gtcgcctcct tgaggctaga catctacttt ggcccgctca cctacgacca
     1861 tagaaacaca atagccttga cggatggatg ccggaagtca acttcgacac tgcggtttga
     1921 ggcaaaaaac ggcgtggttc tcgaatggga ataccccaac cgaatcaagt tgggggtttc
     1981 ttggtgatca gcagcacggg tatgctcacc aactactgcc tttgacatcg gggaaatggt
     2041 actttagcac attcatcgtg ctttcatccc acccccaacc ccgccaggcc acacgcatgc
     2101 gcgtggcctg gcatattcct tcccacgttc cgcatgaagc agatggctgc caatagcccc
     2161 aagccctatg ctgcccaagc aactcgcaag aatatcttgg cccaatcaaa ctagaagagc
     2221 ttcagctaca agtaatacag atccgataat tgcgcagcac ctatccaagc ttgctgtgtt
     2281 aggcagtttt cgataactca atgcgcttgt gggcgcgaca gacgcaaaga cgggtttacc
     2341 cgtgtcatgc acaagctcgt agaaaccgtt ctgttctgca ggaaacgaca ctatgacaga
     2401 atccacatat ggaatggctc tgccatataa tctaaatcgt atgggtaagc tttattaaca
     2461 cgctcgatat aactttctta tattttttcg ataagtcgct tgacaccgat gctgtagttc
     2521 atctataatg taacaggaac ccaaagtatc cgaggtgacc gaagttgagg ctacacggcc
     2581 ctgatgaacc agtctacacc attggcgtag ctgctcgttt gctaggagta agcccgcaaa
     2641 ccctaaggat gatcgagcgt gaaggtttgc ttgaacctgc ccgaaccgaa gcaaacacga
     2701 ggctttactc cgaaaacgac ctgctgctct tgcagcgaat ctgtttcttg gttcgggaag
     2761 agggcgtcaa cctcgcgggg gtcaaaatga tcctcgagat tgaatcaaaa caagggaaca
     2821 caacaatcag gtatcgcgag gttacgcaga gcgttccccg cgaagccaag cctggagaag
     2881 gcaacggcaa ctcctgagtt agggtttcca ttgttccagt tttctagttg agcaacggaa
     2941 ggggagaatc acaatgggga aagttgttgg cattgacctc ggcacaacaa actcagtaat
     3001 tgctgtgatg gagggtggga accccacggt aatagtgaat tcggaaggag cccgattaac
     3061 cccgtcggta atcggcttca gcaagagcgg cgaaaggctc gtcggtcaac ttgcacgccg
     3121 acaagctgtt atgaatcccg aaaacactgt gtattctatc aagagattta tgggacgtcg
     3181 cttcgacgag gtcgaggagg aacgcaatcg cgtaccttac cacgttaaag cctcctcgaa
     3241 ttatggcatc aaaatcgacg tgccggcaac tggcagggat tactctcctg aagagatctc
     3301 tgcgatgtta cttcaaaagc tgaagacgga tgcagagaaa ttcctgggcg aaccgattac
     3361 caaggcggta attacagtcc ctgcctactt caatgattca cagaggcagg caaccaaaga
     3421 cgcgggacgc atcgccgggc tcgaagtgct gaggatcata aacgagccaa cagccgcagc
     3481 gcttgcatat gggctcgaca agaagaaaaa cgagacgatc ctcgtctggg acctcggcgg
     3541 aggcacattc gacgtctcca ttcttgaggt gggcgaaggg gtattcgagg taaagtctac
     3601 cgcaggcgat actcacctgg gcggagacga ctacgaccaa agattggtcg aatacattgc
     3661 cgacgaattc cagcgcgacc agggcatcga tctgagaaag gatcgtcagg cccttcaaag
     3721 gctgatagaa gcggcggaga aggcgaagat cgagctttca caggtttatg agaccaatat
     3781 cagtcttccg ttcatcacgg cagaccagac gggtccaaaa cacctggaaa cgaagatcac
     3841 tcgggcgaag ttcgaagagc tcacatcgga ccttacagag agatgcacta ggccgttcaa
     3901 gcaagcgcta agtgatgcaa agctctcctt agacgacatc gatgaagtga ttctcgtggg
     3961 tggcgccacg cgtatgccag tcattcagga actggtcaag aggcttacag gcaaggagcc
     4021 tcaccgagga gtgaacccag acgaagtagt ggctgtcggc gcggcaatcc aggcaggcgt
     4081 cttagccggt gaagtaaagg acgtagtcct gctcgacgtc acccccctct cccttgggat
     4141 cgagaccctt ggcggagtct ccaccaagct catcgagagg aacactacga tacctacgag
     4201 gaagagccaa atctttacga cagcagagga cgggcagacc tcggtagaga ttcatgttct
     4261 tcagggcgag cgcgaattcg cgcgcgacaa caggacgctg ggtcgattta cactgcaggg
     4321 catcccgccg gcgcctcgcg gagttccgca gatcgaggtg tcattcgata tagacgccaa
     4381 cggaatagtc aatgtcgcag ccaaggatct tggaaccggg aaagagcaac gcatcacgat
     4441 cactgcatca acgcagctgt cgaaggatga agttgaaaac ctggttcgag aagcggaacg
     4501 ggctgctgca gaggacaaga aacgccgtga ggaagtggag ctcaaaaact ccgccgacag
     4561 tctcgtctac gtgacagaga agacgctgcg ggaaatgggc gacaaagtac ctgcagagac
     4621 aagatccaag gtcgaagcgg aacttgagga actgaa
//
LOCUS       DAOSAI010000188         4645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11679_length_4645_cov_3.505011, whole genome shotgun sequence.
ACCESSION   DAOSAI010000188 DAOSAI010000000
VERSION     DAOSAI010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11679_length_4645_cov_3.505011"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..422)
                     /locus_tag="PLM83_07015"
     CDS             complement(159..422)
                     /locus_tag="PLM83_07015"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOK71189.1"
                     /translation="MPDGEVFFPLWRIERKAGAEKNEEFLMEMLDTDPGARYIGEFGI
                     GSNMGITKFTRDILFWDMIADLRKGGEIQLDRVPVQRNGTFLK"
     gene            complement(426..998)
                     /locus_tag="PLM83_07020"
     CDS             complement(426..998)
                     /locus_tag="PLM83_07020"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOK71190.1"
                     /translation="MDPRVWELTKTLVDYSVSVQDGDVVIISADLDGSQLVEAVYRRV
                     LQRGGHPRFKVSMPEVTRILYEEASQEQLATLLPIDLAEYEASDCLISISETANVSPA
                     KMGVRRRAVQPVSDWLMKSSVRWCCCNFPCNTLAQKAEMSLGEYEDFVFGATNVDWPE
                     IIEAPSGGRKEATDLRFSIVDWPGINCDGH"
     gene            1317..3875
                     /locus_tag="PLM83_07025"
     CDS             1317..3875
                     /locus_tag="PLM83_07025"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOK71191.1"
                     /translation="MQDRKHSKARNKESSRKRRGGAGAPTARQKQQPGPAKSRSRKSS
                     DLHGPGNAKRPTQHGSEHRRKKELSRSVAAFAPDIEFPVSEEERAAVPVEYRGLKLDP
                     FQQEAIRHLEAGRSVLVSAPTGVGKTLVADYLIERMYNEKSRVIYTAPIKALSNQKFK
                     EFKRLLGEENVGIITGDVVINSSAQILIMTTEIFRNMLHLSPHALDGVSHVIFDEIHF
                     ISDEDRGTVWEESIIFMPEQMRLLGLSATIPNAGELACWMREIKGHDVAVVRHFKRVV
                     PLRHFVYEKSRGACTLKEMRQYKAERDREIEEAEALAAGAGPRYQTTTHVDLVNYLTE
                     EQKLPCLYFVFSRRQCEEKAEELARTANYLSFAERKEVLSFFDERIEGMNIESMPSVK
                     KMRRILGRGIAYHHAGLLPVLKEIVEDLFERRMVQVLYATETFAVGINFPVKTVCFDG
                     VRKFNGVDFAPMSAQEYFQMAGRAGRRGIDPEGYVYILVDLNYFRPEEYPSTDERSVE
                     PLVSRFTISYNTVVNLIATRSPEEIRKVLKQNFASYQRDAERLEIEEALSSARAMESE
                     IIAEACELLDKPGCPLYAGKLRSRLAETRVQLKRASKRKRSRGKRAALRVEMDEIRAE
                     LGSLDTRECEVAQKKACRKLLSKRGVVVGRMENLYERMRRLGSEERYVHEFRRKQALL
                     ERLSYVQDGVLLPRGEICREIHTQELLVTELIFAGVFHEFDIDQIAALAVCIDYEPRR
                     GEFAAKSVPFDLARIREIAEAIEVVEESFVGTSSLQFFTGLAPLAYRWSQGEDFADLM
                     KGSSYAEGDVVSSFRRAIDLLRQVRAACKDDLALADKITETIRRIDRDVVEVVL"
     gene            complement(3964..>4645)
                     /locus_tag="PLM83_07030"
     CDS             complement(3964..>4645)
                     /locus_tag="PLM83_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71192.1"
                     /translation="VLDDWSDANAEALDAGAANKLCHQIIVDVFGQTSVADRPGKKRS
                     LFASAITPDGFVNYLDTVVAGCLRRFVIHGGPGSGKSTLIGHVADHAIRSGLDVELFH
                     CSLNPESLEHLVIPAIKTALITSVEPHVWTPRKDDIVIDMEQCLDRTVVERNARIVDY
                     DRSLMQEAMDRAIFFLGQARDAHRFLESLYTPNIDFDAVKGLRERTLNRILEYASESR
                     QVAPEHGN"
BASE COUNT         1086 a         1243 c         1295 g         1021 t
ORIGIN      
        1 gcagagtctg ccaacttgcg tgtcccgtta tcggtatttg gcgaaaataa ctccaatatg
       61 ggccttttgg cgttaggcaa aaaagaagca tcttatccca tcagaacgct gcgatttggc
      121 ttcggcatgt ttgagggggc agtgctgact gaagaatcct acttcaggaa tgtcccattt
      181 cgttggacgg gcaccctatc caactggatc tcgccgccct ttcgaaggtc tgcgatcata
      241 tcccagaaga gaatgtcccg ggtgaacttg gttatcccca tgttcgaccc gatcccgaat
      301 tcccctatgt acctcgctcc cgggtcggta tcgagcatct ccatgagaaa ctcctcgttc
      361 ttctcagcac cggccttccg ctcaatcctc cacaggggaa agaagacctc gccatcgggc
      421 atattttaat gtccgtcgca gttgatgccc ggccagtcga ctatggagaa ccgtagatca
      481 gtcgcttctt tgcggcctcc acttggcgct tcgataatct cgggccagtc cacgtttgta
      541 gctccgaaga cgaaatcctc gtactcccct agcgacatct cggccttttg agccagtgta
      601 ttgcatggga agttgcagca gcaccacctc acactgctct tcatcaacca atcggagaca
      661 ggctgtacgg ctcggcgcct aacccccatt ttggcagggc tcacattcgc agtctcggat
      721 atgcttatca ggcaatccga tgcctcatat tccgcgaggt cgatcgggag caaagtggcc
      781 aactgctctt gtgatgcttc ctcatagaga atccgcgtca cctcgggcat ggagactttg
      841 aacctaggat gtcctccccg ttggaggact cttctgtaca ccgcctccac cagctgcgaa
      901 ccgtcgagat ccgcgctgat tatcaccaca tcgccatctt ggacactgac tgagtagtct
      961 acaagagtct ttgtcagctc ccacaccctc gggtccataa tcattacctc caattccgca
     1021 tcggcttttc ctatagcaat ttttcagaag aggctccggg tttttctgca ggcgaatagg
     1081 attgtctccg agcctccgcg aaacgcgcct cgattgcgat gccttgaata gttggggctg
     1141 gcatggggct gtactaaacc atatagaccg ggctgggcgg tgcgaccgct ttcgaatatc
     1201 gccgactgaa ttgtcccagg caccgagaac ctgtatactc aagatggcag ccagggcttg
     1261 cctttcaaag cagagcggac tcgggtccgc gttgtctcta ctgaggtgat aaattaatgc
     1321 aggatcgaaa acattccaag gcacgcaaca aggagtcgtc gcgcaagaga cgcggcggag
     1381 cgggcgcgcc gacggctagg cagaagcaac agccgggccc ggcgaagagc cgttcaagga
     1441 aatcttcgga cttgcacggc cccggcaatg ccaaaaggcc gacacagcat ggctcggaac
     1501 atcgacgcaa aaaggaattg tcgcgttctg tggcggcttt tgcgccggat atcgaattcc
     1561 ctgtttccga ggaggagcgc gctgctgtgc ccgtagaata caggggattg aagctcgatc
     1621 ccttccagca ggaagccatc aggcatcttg aggccggaag atccgtcctc gtttctgctc
     1681 ctacaggcgt cggcaaaaca ttggtggccg actatctcat cgagcgtatg tacaacgaaa
     1741 agagcagagt gatatatact gcgcccatca aggccttgtc gaaccagaaa ttcaaggagt
     1801 tcaaacggct tttgggcgag gagaacgtcg ggatcataac gggcgacgtc gtcatcaact
     1861 catcggccca gatactgatc atgaccacgg agatcttccg gaacatgctc catttgagtc
     1921 cccacgccct agatggcgtc tcccacgtaa tattcgacga aatccacttc atttccgatg
     1981 aggaccgcgg cacggtgtgg gaggagtcga ttatattcat gccggagcag atgaggctcc
     2041 tcgggctctc tgcaaccata cccaacgcag gcgagctggc ctgctggatg cgggagataa
     2101 agggacatga tgtggcagta gtccgccatt ttaagcgcgt agttcccctg aggcacttcg
     2161 tgtacgaaaa atcgcgcggc gcatgcacat tgaaggagat gaggcagtac aaggccgaac
     2221 gagaccggga aatcgaagag gccgaagctt tggcggctgg ggcgggcccg agataccaga
     2281 cgacaaccca tgtcgacctc gtgaattatc tcacggagga acagaagttg ccatgccttt
     2341 acttcgtctt cagccggaga cagtgtgagg aaaaagccga agaactggca agaacggcaa
     2401 attacttgag ctttgccgaa cgaaaagaag tcctttcctt tttcgatgag agaatcgaag
     2461 gaatgaatat cgagtcgatg ccgagcgtta agaagatgcg ccgaatactg ggtaggggga
     2521 tcgcatacca tcacgccggg cttttgccgg tcctcaaaga gatcgttgaa gacctcttcg
     2581 agcgcaggat ggtgcaggtt ctctacgcta ctgagacctt tgccgtgggc atcaacttcc
     2641 ctgtgaaaac tgtgtgcttt gacggagtcc gcaagttcaa cggcgtcgat ttcgcgccca
     2701 tgagcgccca ggaatacttc cagatggcgg ggcgcgcggg caggaggggc atagaccctg
     2761 aaggctatgt gtacatactc gtggacctca actatttccg tcccgaagaa tatcccagca
     2821 ctgatgaacg aagcgtcgag cctttggtga gccgatttac catatcatac aacaccgtcg
     2881 tcaacctgat cgcgacccgg tcgccggagg aaatacgcaa agtcttgaag cagaacttcg
     2941 ccagctatca gagggatgcc gagaggttgg agatagagga ggccttgagc tctgcgcgag
     3001 caatggaatc cgagatcatc gcggaagcgt gcgaacttct ggacaaaccg ggatgtccgc
     3061 tctacgccgg aaaactccgt tcccgtctcg cggagacccg ggttcaactg aagagagcct
     3121 ccaaaaggaa aagaagccgc ggcaaacgcg ctgcgctcag agttgaaatg gacgagatcc
     3181 gagctgagct cggttcactg gatacccgcg aatgcgaggt cgctcagaag aaggcctgcc
     3241 gaaaactgct gtctaagagg ggagtcgtag tcggccgcat ggagaatcta tacgagcgca
     3301 tgcgtaggct aggtagcgag gaaaggtatg tgcacgagtt cagaaggaaa caggcactcc
     3361 ttgaaagact cagttacgtg caagatggcg ttcttctgcc taggggggaa atatgccgcg
     3421 aaatacacac acaggaactc cttgtgactg agctgatttt tgcaggcgtc tttcacgagt
     3481 tcgacataga ccaaatcgct gcattggcgg tttgcatcga ttatgagccg cggcgcggag
     3541 agttcgctgc gaagtcagtc ccgttcgatc ttgcccggat ccgtgaaata gccgaagcta
     3601 tagaggttgt tgaagagagc tttgtaggca caagctcact ccagtttttc accggccttg
     3661 ccccgcttgc ttacagatgg agccagggcg aggacttcgc tgacttgatg aagggttcaa
     3721 gttatgccga aggcgacgta gtctccagtt tccggcgggc catcgacctt ctccgacaag
     3781 tgcgggcagc gtgcaaagac gacctcgctc tggctgacaa gataacggag accataagac
     3841 ggatcgacag ggatgttgta gaggtagtgc tatagaaatt atctcggctt tcattaggca
     3901 actaataccc caacaacagg tagcgcacat gccaggattc cgcagtgaac tgttgggacc
     3961 tgttcagttc ccgtgctccg gggcaacctg gcgagattca cttgcatact cgagtattct
     4021 attgagagtt cgttccctta gccctttgac agcatcaaaa tcgatgttgg gagtgtatag
     4081 ggattccaga aaacggtgcg cgtcccgcgc ctggccgagg aagaatattg ctcggtccat
     4141 cgcctcttgc attaggctgc ggtcgtaatc cactatcctt gcgttgcgtt ctacgactgt
     4201 tcgatcgaga cattgctcca tgtctatgac gatgtcgtct ttgcgagggg tccacacgtg
     4261 cggttcgaca gacgtgatca gtgctgtctt gatagcaggt atcacaaggt gctcgagact
     4321 ttcggggttg agcgaacaat ggaaaagctc cacatctagc cctgaacgga tcgcgtggtc
     4381 ggctacgtgc ccgatgaggg tagattttcc ggatccaggg cctccgtgga tgacaaaccg
     4441 cctcaagcag cctgctacca ccgtgtcgag gtagttgacg aagccgtccg gtgttatcgc
     4501 cgatgcaaag aggctgcgtt tcttgccggg gcggtcagcc acgctcgtct gtccgaaaac
     4561 gtccacgatt atctggtggc agagcttgtt ggcggcacct gcatcaagcg cttcagcatt
     4621 tgcgtcgctc cagtcatcta gcacg
//
LOCUS       DAOSAI010000189         4630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11732_length_4630_cov_3.678033, whole genome shotgun sequence.
ACCESSION   DAOSAI010000189 DAOSAI010000000
VERSION     DAOSAI010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11732_length_4630_cov_3.678033"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..2369)
                     /locus_tag="PLM83_07035"
     CDS             complement(192..2369)
                     /locus_tag="PLM83_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOK71193.1"
                     /translation="MHSNERGSLQVVGIVILLLILVGIGVFSGILAGYLKNAPSVEDV
                     DIESHRSLPTVIYDAGGQVLTRLMVENRAWVPLSSVPSDLVNAIVAVEDHTFYDHHGI
                     SLRRIAGALWHDLKHMSPDQGGSTITAQLARNVFLSQEKTIDRKIWEALYAFQLERRY
                     TKDEILEYYLNWIYMGHGVYGVEAASQLYFGKPVTELSLGQCALIAGLAKGGEVYSPY
                     KNMEAALQRRQIVLKRMADVGYISPEEASIAADEPVKLVGLKESGNTVGKYVRYMVRD
                     YLTGKYGKDAVYRGGLTVKTTIDLKAQQAAEQAFTSLLPTGRIEEREGYSLTYPQCAL
                     VAIDPQTGAIRALVGGRGEDEYNRATKAMRSPGSSMKPFVYTAAIDSGYTPATTMVDE
                     PISYPMGDGSMWSPKNYNGKYNGVMTLRQALEQSTNTIAVKLAKELGPETVIHYAKKM
                     GITSLVERGAKNDKGLALALGGLTQGITPIEMAQAYSVLANGGVKVTPYLVTEVRDAN
                     GVVLESHGPNREIVLDERTAYIMADMMKGVITSPNGTGKRADIGRPAAGKTGTADSNT
                     DAWFVGFTPDLAAAVWIGEDTLREMRYPNYGVVGSGKAAEIWGSFMKQALAGTAPRDF
                     TVPEGIISGVRVCKVSGDLATSNCPSGSVIYEKFIAGTEPTRSCRVHGGYAKPKTEPE
                     SEPRLENDPFDQVQAVPQTPPYTDSEASPQIEYSDSQTPPWEQ"
     gene            2543..3763
                     /gene="tyrS"
                     /locus_tag="PLM83_07040"
     CDS             2543..3763
                     /gene="tyrS"
                     /locus_tag="PLM83_07040"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOK71194.1"
                     /translation="MNPKEQLEIIERGAAEVISVEDLLAKFERSAKTGKPLKVKLGLD
                     PSAPDIHIGHTVVLYKMRQFQDMGHEGIIVIGDFTGRIGDPTGRSEMRKQLTEEQVRA
                     NARTYEEQIFRILDPARTRVTFNSEWLSKLGFADVIELASKVTVARMLERDDFENRYK
                     EGRPIGIHEFFYPLMQAYDSIALEADVELGGTDQKFNNLMGRTLQREYGQDPQCVVLT
                     PLLPGLDGVNKMSKSLGNYIGIDEEPFEIYGKTMSIPDELIVAYVDSATTMSVAEKKG
                     IEEGLASGTLHPMTAKRRLAFQLVLQFHGEDAARGAQDEFDRVFKHGEHPDEMPEVRV
                     SQDDLVDGKIAVSKLVVIAGLASSNSEARRLIEQGGVRIDDVRVEGVMDEVEPVNGMV
                     INVGKRRYAKVIRE"
     gene            3791..>4630
                     /locus_tag="PLM83_07045"
     CDS             3791..>4630
                     /locus_tag="PLM83_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOK71195.1"
                     /translation="MASKEILDAANEGRYAVGAFNVNNLEFAKGVVSAAEEERSPAII
                     QVSESAIAYAGIEEIACIVRSLAKDVGVPIALHLDHGRKFDVIMQCIRHGFTSVMIDG
                     SNLPLDENIAITKKVVEAAHGAGVSVEAELGRLAGIEDDINVQARDAFLTDPEEAAIF
                     VRETGVDALAIAIGTSHGPRKFKGEPVLALDLVTEIKEKVGIPLVLHGASGVSTDVIE
                     QAEKYGAKWGGSKGVPDESIVEAINRGINKVNIDTDMRLAFVASIRETLSTRPEVTDP
                     REVL"
BASE COUNT         1056 a         1117 c         1334 g         1123 t
ORIGIN      
        1 aaactatgat ccataaatcc aaaaccttga tgtccaaggg tgccgaagat cggccggagc
       61 ttagcgattg gcgagcgagg cgtcgatggt tggcagcaaa cagagcccgg ggtcttgtca
      121 gacctcgggc tttcctgtat gtgtgtttca tgttcttgtt aacgatctgt ttcggttgcg
      181 gtttggaagg ttcattgctc ccatggcggc gtctgcgaat ctgagtattc gatctgcgga
      241 gaggcttcgc tgtcggtgta gggtggagtt tggggcacag cctgcacttg atcgaaagga
      301 tcattttcga gacgaggttc gctttcaggc tcggtcttgg gcttcgcata tcccccatgc
      361 actctgcaag aacgggtcgg ttctgtgcct gctatgaatt tctcgtagat gaccgatcct
      421 gatgggcaat ttgatgttgc caaatcacca gaaaccttgc acacgcggac acctgagatt
      481 attccttcag gtacggtgaa atcgcgcgga gcggttccag ccagagcttg cttcatgaag
      541 gagccccaaa tctccgcggc cttcccgctt ccgacgacgc catagttggg ataccgcatc
      601 tccctgagag tgtcctcgcc tatccagaca gctgcagcca ggtcaggtgt gaatccgacg
      661 aaccatgcgt ccgtattgct gtctgccgta ccggtctttc ctgcggcagg tctgccaatg
      721 tcggcgcgct ttccagtgcc gttcggggag gttatgacgc ctttcatcat gtccgccatg
      781 atataggcag tcctttcatc taatacgatc tccctgttgg ggccgtgtga ctcgaggact
      841 actccgttgg catcccgaac ttcggtgacg aggtagggcg tgaccttgac tcctccgttc
      901 gccaacacgc tgtacgcctg cgccatttca ataggggtga tgccttgggt tagtccgcct
      961 aaggccaggg ccaacccttt atcattcttg gcgcctcttt ccacaagaga tgtgattccc
     1021 atcttctttg cgtagtggat cacagtctct ggtcccaatt ctttggccag cttgacagcg
     1081 atggtgtttg tcgactgctc gagcgcttgc ctcagcgtca tgacgccgtt gtatttcccg
     1141 ttgtagtttt ttggagacca catcgagcca tctcccatgg ggtatgagat tggctcatca
     1201 accatggttg ttgcaggagt gtaacctgag tcgatcgcag ccgtgtaaac gaatggcttc
     1261 atggacgaac cgggagaacg catggctttg gtggcccggt tatactcgtc ttcacctcgc
     1321 cctccgacga gggcccttat cgccccggtc tggggatcga tagcaacaag ggcacactga
     1381 gggtatgtca gcgaataccc ttcgcgttct tctattctgc ctgtcggcag caaactcgtg
     1441 aatgcctgct ctgccgcctg ttgggccttg aggtcgatcg tggtctttac tgttaggccg
     1501 ccgcggtaaa ctgcgtcctt tccatatttg cctgtcaagt aatcgcgaac catgtacctt
     1561 acgtattttc ctactgtgtt gccggactct ttcaatccga cgagcttgac tggctcgtct
     1621 gccgcaatcg aagcttcctc tgggcttata taacccacgt cggccatgcg cttgagcact
     1681 atctgccgtc tttgcagcgc tgcttccatg ttcttgtaag gagagtagac ttcgccgcct
     1741 ttggcgagtc cggcgataag ggcgcattgg ccgagagaca gctcagtcac aggcttgcca
     1801 aagtaaagct gtgaggcagc ctcaacccca tagacgccgt ggcccatata aatccaattg
     1861 aggtagtatt caaggatctc gtcctttgtg tatcttcgct cgagctgaaa agcatacaag
     1921 gcctcccaga tcttgcggtc aatggtcttc tcttgcgaga ggaaaacatt cctggctagc
     1981 tgggcagtta tagtgcttcc gccttgatcg ggcgacatgt gcttgagatc gtgccacagc
     2041 gccccggcaa ttctgcggag gcttatgccg tggtgatcgt agaaagtatg gtcctcaaca
     2101 gcgactatcg catttaccaa atcagaggga acgctgctca acgggaccca tgctctgttt
     2161 tcaaccatga gtcgtgtaag gacttgaccg cccgcgtcgt atatgactgt aggaaggctg
     2221 cgatgagatt ctatgtcaac atcctccacg ctaggagcgt tcttgaggta ccctgccaag
     2281 atccccgaga acaccccgat gcctacgagg atgagaagga gaatgacaat gccgacaact
     2341 tggagagaac ctctctcatt cgaatgcatg atgattgaaa ggcctcctcg cctcaataat
     2401 tctgctacat tataacatag tgatttacat gcgcaaacac tggaccggag gggcgactgg
     2461 aagcggctgg tcttcattga cccaggttgc tgccctgtgc tagactcaat gtatagttgt
     2521 agatttcggg gggcattggg ctatgaatcc caaggaacag ctggagatta ttgaaagagg
     2581 cgctgcagag gtcatctccg ttgaggacct tcttgccaag tttgagaggt cggcaaagac
     2641 aggaaagcct ctcaaggtga agctggggct tgatccgtct gctcctgaca ttcatatagg
     2701 ccacacggtg gttctgtaca agatgaggca gtttcaagac atgggccatg aggggatcat
     2761 cgtaataggg gatttcaccg gtaggattgg cgatcccacc gggaggtcgg agatgcgcaa
     2821 gcaactgact gaggaacaag tgcgcgcgaa tgccaggacg tatgaggagc agattttcag
     2881 gattttggat ccggctcgca cgcgtgtgac gttcaactcg gaatggcttt cgaaactagg
     2941 tttcgctgat gtgatcgaac ttgcgtccaa agtcacagta gcacggatgc ttgaaaggga
     3001 tgatttcgag aacaggtata aagaaggtcg cccaattggg attcacgagt ttttctatcc
     3061 tttaatgcag gcttatgatt caattgcact tgaagccgat gttgaactcg gaggcacaga
     3121 tcagaagttc aacaacctga tggggcgcac cctccagcgg gaatacgggc aggatcccca
     3181 gtgtgtggtg cttacgcctc ttctccctgg gcttgacggc gtgaacaaga tgagtaagag
     3241 cttgggcaat tacataggga ttgatgagga gccgtttgag atctatggaa agaccatgtc
     3301 aattccggac gagctgattg tggcatacgt cgattcggct acgacgatgt ctgtggctga
     3361 gaaaaaggga attgaggaag gtttggcatc agggacgctc cacccgatga cggcaaagcg
     3421 gcgcctggct ttccagcttg ttctccagtt tcacggagaa gatgccgcta ggggcgcgca
     3481 ggatgagttt gacagggttt tcaagcacgg cgagcatccc gacgagatgc cagaagtaag
     3541 agtatcacag gatgatctcg tggatggtaa gattgccgtt tccaagctcg tggtcatcgc
     3601 tgggttagct tccagcaaca gtgaagccag acgcttgatt gagcagggtg gagtgagaat
     3661 tgacgatgtc agggtagagg gcgtcatgga cgaggttgag cctgtaaatg gcatggtgat
     3721 taacgtgggc aagagacggt atgcgaaggt cattagagaa taaagtgcgg aggtggccaa
     3781 attgagtctt gtggcctcca aagagatttt ggacgcggcc aatgagggca ggtatgctgt
     3841 aggcgctttc aacgtcaaca accttgaatt cgcaaagggt gttgtgagcg ctgcagagga
     3901 ggaacggtct ccagcaatca tccaggtgtc tgagagcgct atagcctatg caggcattga
     3961 ggagatagcg tgcatcgtaa ggtctctggc gaaggatgtc ggagtcccca tagcgcttca
     4021 ccttgatcac ggacgcaagt tcgatgttat catgcagtgc ataaggcatg gatttacctc
     4081 tgtaatgata gatggctcca atctgccatt ggatgagaac atagctatca ccaagaaagt
     4141 ggtggaagct gcccacggtg ctggagtcag cgttgaagcg gaacttggac gattggctgg
     4201 aatagaagat gacataaatg tccaggcaag agacgccttc cttaccgatc cagaagaggc
     4261 cgccatattt gtccgggaga ccggggtcga tgctttggca atagccatag gcacatcgca
     4321 cgggccgagg aaattcaagg gcgaaccagt attggcgctc gacctagtga cagagataaa
     4381 ggagaaagtc ggcattcctc tggtgcttca tggggcttca ggggtatcca ctgatgtgat
     4441 agagcaggca gagaaatacg gcgctaagtg gggcgggtcc aagggagttc cggacgagag
     4501 catagttgaa gctattaatc gcggcataaa caaggtaaat atcgacacag atatgaggct
     4561 agcctttgtg gcgagcataa gggagacgct ttcaaccaga cctgaggtta cggatcccag
     4621 ggaagttctg
//
LOCUS       DAOSAI010000190         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11739_length_4628_cov_3.504702, whole genome shotgun sequence.
ACCESSION   DAOSAI010000190 DAOSAI010000000
VERSION     DAOSAI010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11739_length_4628_cov_3.504702"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /gene="purD"
                     /locus_tag="PLM83_07050"
     CDS             <1..1182
                     /gene="purD"
                     /locus_tag="PLM83_07050"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOK71196.1"
                     /translation="NAGIERLGQCVPIDAADVEGLARFAQEADCHLVVVGPELPLSLG
                     LADRLAAQGIAVFGPTASAARIESSKAYAKGLMERAGIPTARYGVFDDISKARQAAVA
                     IASDAGRVVVKLDALAAGKGVIVASGPEEAGAAVDSLAESAKASNCRLLVEECLEGYE
                     ASVIGITDGKDVVTLVPARDHKRAFDGDEGPNTGGMGAVAPAPGIDRAMLDWISNKVL
                     LPAVRQMADEGDPFVGALFAGLMITDEGPKVLEFNCRFGDPETQAIMPLLESDLLDLV
                     EAACHGRIGEMKADFVPGYSTCVVLASNGYPGPYSTGVPVRVPSELPEGTVIFHAGTR
                     ISEGQLVSDGGRVLGVTAVGSTQTESRRRAYDAVERIGFEGGYCRSDIGLDVRFTGGG
                     L"
     gene            1298..1591
                     /gene="yqfC"
                     /locus_tag="PLM83_07055"
     CDS             1298..1591
                     /gene="yqfC"
                     /locus_tag="PLM83_07055"
                     /inference="COORDINATES: protein motif:HMM:NF019488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HOK71197.1"
                     /translation="MREARSARIKERMSDALGVPKDVALDLPRLSLEGNARLVMENHK
                     GLIEYSADRLCIQTTVGQVSIVGRKLVLRGINRERIVVEGRFDSLEFLGWEVS"
     gene            1593..2750
                     /gene="yqfD"
                     /locus_tag="PLM83_07060"
     CDS             1593..2750
                     /gene="yqfD"
                     /locus_tag="PLM83_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HOK71198.1"
                     /translation="MATSGLWDWVRGYVIIRVTGRMIEKFVNMAAARHIGLYDVTTLG
                     PGMITARVSVDDYRRLHPILRDCQCRATVDARVGAPFLIAALGRRKFLFIGLMLFVAI
                     LYVMSSILWTIEVVGANPHQEQQIRRILDDMGVNRWTLLSSIDCDTIRSEITKSVEGL
                     AWAGVETQGTALIVRVAEKVLPDAPVFPIDLIASEDAIVVKLIVLAGDACVAEGQTVV
                     AGQVLVSGKSASGAGGDAAHARAIVEGRVWREGRAVVPLNVEERVRTGRKSIHYRVLW
                     GNLDFRLGRVGSFEAFDTKEYRRDYRPGVEFIRIERYELALTSTRISRSEAYALARAA
                     AEDSIAAKLNKDARIVSVSERVSDENVMPEAVEVILTVETVEDIARPRERQ"
     gene            2899..3909
                     /locus_tag="PLM83_07065"
     CDS             2899..3909
                     /locus_tag="PLM83_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOK71199.1"
                     /translation="MSNPVVEARFCTNDNSEAAALFGNRDENLRLVEELLECKIVARA
                     NDVTVIGPEDSVSKVMRVLSDLQSAVRAGNRISGRETRYSVMLAQNERPETLCSVLRD
                     VVQVTARGKQVRPRTVGQKHYVDAMRRNDIVFCIGPAGTGKTYLAMAMAVSVLKAKNV
                     SRIILTRPAVEAGEKLGFLPGDLQEKVDPYLRPLYDALFDILGVEMFERYKEKGIIEI
                     APLAYMRGRTLDDAFIILDEAQNTTPEQMKMFLTRLGFSSKAVVTGDITQIDLPNRKF
                     SGLEDVQTVLAGVDGIEFVHLNDQDVVRHELVQKIVQAYERYDAVRAKKEQLGVDLSR
                     GE"
     gene            3945..>4628
                     /locus_tag="PLM83_07070"
     CDS             3945..>4628
                     /locus_tag="PLM83_07070"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71200.1"
                     /translation="MAQLSRKESARRFGSSVKTFASSRIAVAVATVILGSLIVASALL
                     PQAVTLEEGQFAKRDIQAQRTIVNRVATEKLREEARKTYLKNAPSFPDNYEINQSCSY
                     MSEEAVESVFSAVDSARSEMPDEEDLAAVGRRAAAIIDDGEGISVSAEDLALLASMDE
                     AEREDARDIAVALVGSVMRENRITDSNLQEIAAGIPEALLEKGVAPEYVAPISRIVVS
                     AIRPNLSLSL"
BASE COUNT         1015 a         1065 c         1478 g         1070 t
ORIGIN      
        1 aatgcaggca tagagcggct gggccagtgt gtcccgattg atgctgctga cgtagagggg
       61 ctggcaagat ttgctcagga agccgattgc catttggtcg tagtagggcc tgaactgcct
      121 ttaagcctcg gccttgccga taggctcgct gcccagggga ttgcagtgtt tggccctaca
      181 gcgtccgctg ccaggattga atcatcaaag gcctatgcca agggcttgat ggagcgggct
      241 ggcataccta ctgctcgcta tggagtcttc gacgatatct ccaaggcgag gcaggctgcg
      301 gttgcgatcg catccgacgc tggaagggta gttgtgaagc tggatgcgct tgctgccggc
      361 aaaggcgtga tagtcgcctc aggcccagag gaagcgggag cagccgtgga ttctttggct
      421 gaatctgcga aagcaagtaa ctgccggctg cttgtggagg agtgccttga gggctacgag
      481 gctagtgtta taggcataac tgacggcaaa gatgtcgtga cgctcgtgcc tgccagagat
      541 cacaagcggg cttttgacgg tgatgaggga ccgaatacgg ggggcatggg cgccgtggcg
      601 cctgctcccg gcatcgaccg ggcaatgctc gattggatca gcaataaggt gctgcttccg
      661 gctgtcaggc agatggcgga tgagggcgat ccatttgtgg gcgccctctt tgccgggctg
      721 atgataacgg atgaagggcc aaaggtgctc gagttcaatt gcaggttcgg cgatcctgag
      781 acccaagcca ttatgcctct tctcgaaagt gatctgcttg accttgtgga agctgcatgt
      841 cacggtcgca tcggagaaat gaaggcagac tttgtacctg gctattccac ctgtgtagtg
      901 ttggccagta atggatatcc aggtccttat agcactgggg tgcctgttcg agtgcctagc
      961 gagcttcctg aaggcacggt aattttccac gcaggcacga ggatctccga gggccagctt
     1021 gtctcggacg gcggtagggt gttgggagtc actgctgtcg gatctaccca aactgagtcg
     1081 cgtcggcgcg catacgatgc ggtggagcgc atagggtttg aagggggtta ttgccgatcc
     1141 gacatcggcc tggacgtccg ttttaccggt gggggcctct aggcaattct caagtttgca
     1201 tcgtccgtgc tagacgaagg cacttcttgc gagtcactca gaaaacctgg acgggacggc
     1261 ttgcctgagc cgctccgtga atagcggagg tattcacatg cgtgaagcgc gttcggcccg
     1321 cataaaggaa cgaatgtctg acgcgcttgg cgtgccgaag gacgtagccc tcgatttgcc
     1381 tcggctcagt ttggagggca atgccaggct cgtgatggaa aaccacaagg gccttatcga
     1441 gtattctgct gatcgcttat gtatacagac tactgtcggc caagtatcaa ttgtcggtcg
     1501 caagctggtc ctgagaggaa taaacaggga gaggattgtg gttgaaggac gctttgatag
     1561 tcttgagttc ctagggtggg aggtgtcctg agatggcaac atccggcctg tgggactggg
     1621 ttcgtggata tgttataatc agggtcacag gcaggatgat cgagaaattc gttaatatgg
     1681 cggcggctcg ccacataggg ctgtatgatg tgaccactct gggtccgggg atgatcaccg
     1741 ccagggtgag cgttgatgat tacaggcgcc tgcatccgat tcttcgggat tgccagtgcc
     1801 gagccaccgt ggatgcgcgg gttggagccc cgttcttgat tgcagcgttg ggcaggagga
     1861 aattcctctt catcggtctg atgctcttcg ttgcgattct ctatgtgatg tcttcgattc
     1921 tgtggacgat agaggttgtt ggcgccaatc cccaccagga acagcagatt cgccgcattc
     1981 tcgatgatat gggcgttaat agatggactc ttctgtcatc tatcgactgc gacaccatca
     2041 ggtccgaaat cacgaaatca gtagaaggcc tggcttgggc aggcgtggag actcaaggta
     2101 ctgccctgat cgtgcgcgtg gctgaaaagg ttctgccgga tgctcccgtt ttcccgatcg
     2161 atctgattgc gtcagaagat gccatagttg tgaaactgat tgtccttgca ggcgatgcgt
     2221 gtgtagctga ggggcagacg gtcgtggcag gccaggtgct tgtatcagga aaaagcgcgt
     2281 cgggtgcggg tggtgatgcg gcacatgcca gggctatcgt cgaaggccgc gtgtggcgcg
     2341 agggccgcgc agtagtcccg ctcaacgtgg aagagcgcgt gaggaccggg cgcaagtcaa
     2401 tacattaccg ggttttatgg ggcaacctcg attttcgtct gggcagggtt ggcagttttg
     2461 aagctttcga taccaaggag tatagaaggg actaccgtcc tggcgtagaa tttattagaa
     2521 ttgagcgata cgagcttgca ctcacctcaa cgcgcataag tcgcagcgag gcatatgcgc
     2581 ttgcccgtgc tgctgctgaa gattcgatcg cagccaagct caacaaagat gcgaggattg
     2641 tatctgtgag cgaaagggta tctgacgaga atgtaatgcc agaggctgtc gaggtcattc
     2701 ttactgtgga aactgtagaa gacatagcca gaccgcggga acgccagtag gtggtcgaga
     2761 cgttgcatgt tatagctgga actatatgac gatgaagtgg caggggcaag cgcggtttct
     2821 gcatatttga gagatgatgt tttctagcaa ggctattgct atttcgcatc aactgtgaga
     2881 aggagggagt aatctcattt gagcaatccg gttgttgaag ctaggttctg caccaatgat
     2941 aattcagagg cagctgcact tttcggtaac cgggacgaga atctaaggct agtcgaggaa
     3001 cttctcgaat gcaaaatagt agcccgtgcc aatgatgtga ctgtaatagg gccggaggat
     3061 tctgtcagca aggtaatgcg ggtcctctcc gacttacagt cagctgtacg cgcgggcaat
     3121 cgcatttccg gccgggaaac caggtactct gtgatgcttg ctcagaacga aagacctgaa
     3181 acactctgtt ctgtattgcg cgatgtggtg caggttactg cccgcggaaa gcaggttcgc
     3241 ccgcgaactg taggccagaa gcactacgtg gacgcaatga ggaggaatga catagtcttc
     3301 tgcattggcc cggcaggaac cggcaagact tatctggcga tggccatggc tgtttcggtt
     3361 ttgaaggcca agaacgtcag cagaattatt cttacgcgcc ctgctgtgga ggcgggggag
     3421 aagctcgggt tcctccccgg cgacttgcag gaaaaggtgg acccttactt gcggccacta
     3481 tatgatgccc tctttgacat actcggtgtc gaaatgtttg agcgctacaa ggaaaagggc
     3541 ataattgaga tcgcgccact tgcgtacatg cgcgggagga cattggatga cgcgttcatc
     3601 atacttgatg aggctcagaa taccacgccg gagcagatga agatgttcct gactcgtctg
     3661 gggttctctt caaaggcagt ggttactggg gatattactc agatagacct ccccaacagg
     3721 aagttttccg gcctcgaaga tgttcagacg gtgcttgccg gagtggatgg catagagttc
     3781 gtccacctga acgaccagga cgttgtcaga catgagctgg tacaaaagat agttcaggcc
     3841 tatgagagat acgatgctgt gagggctaag aaggagcaac tcggtgtcga cctgtcccgg
     3901 ggagagtaaa gatggcggag acggaatgag aaggggtgat gccgttggca cagctgtcta
     3961 gaaaagagag cgcgaggcga tttggctcaa gcgttaagac cttcgcgagc agtaggattg
     4021 cagttgctgt ggcgacggtg attttggggt ctctcattgt cgcatcggcg ctgttacccc
     4081 aggcggttac gctggaagag gggcagtttg cgaaacgtga tattcaggcc caaaggacaa
     4141 ttgtgaatcg tgttgctact gagaagctga gggaagaggc tcggaagaca tacttgaaga
     4201 atgctccgag tttcccggac aactacgaga taaatcagtc atgctcttac atgtccgagg
     4261 aagcggtaga gtctgtcttc tccgcggtgg attcggcgcg cagcgagatg cctgatgaag
     4321 aagacctggc ggcggtcggt cgccgtgcgg ccgccataat cgatgatggc gagggcattt
     4381 cggtgtctgc agaggacctt gctcttttgg catcaatgga tgaggctgaa cgcgaggacg
     4441 cccgggatat tgcggttgcg cttgtcggca gtgtaatgcg ggagaaccga ataaccgatt
     4501 ccaatctgca ggagattgcg gcgggtattc ccgaggcgtt gctggaaaag ggtgttgcgc
     4561 ctgaatacgt ggcgcccatt tcgcggatag tcgtttcggc gattcggccc aacctctctc
     4621 tcagcctt
//
LOCUS       DAOSAI010000191         4606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11823_length_4606_cov_3.637442, whole genome shotgun sequence.
ACCESSION   DAOSAI010000191 DAOSAI010000000
VERSION     DAOSAI010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11823_length_4606_cov_3.637442"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLM83_07075"
     CDS             <1..370
                     /locus_tag="PLM83_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOK71201.1"
                     /translation="ALSGKDPTKVDRSAAYAARHVAKNLVAAGVADKCEVQIAYAIGV
                     ARPVSISVDTYGTGAIPDERITRIVREVFDLRPEAIIRKFDLRRPIYRQVSVYGHFGR
                     PDLDLPWEALDMVEEVRKRM"
     gene            520..>1998
                     /locus_tag="PLM83_07080"
     CDS             520..>1998
                     /locus_tag="PLM83_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK71202.1"
                     /translation="MARISTDSGIMTAVGPFSSATPGQAVKLTGDWVVHRSYGRQFRV
                     AKMEVVAPATVEGIEKYLGSGLIPGIGPVTAKRIVEHFAMETLEVIQSCPDALLKVPG
                     IGPKRAAQIVKAVEEHKSIEKVMVFLQSHGVSPTYAAKIYKRYGDDAVAIVSRNPYQL
                     ADDIYGIGFKTADKIAREVGVDKDSPYRVNSGIKYWLAKASDDGHCYSVAEDVIREVA
                     AELDVSEDLVKQGLGALEESGEVFCERESSVWLGPFYHSERGVAKRLWELNHGRMWQL
                     DALSDEELGRAQAEAGLTLGAQQKQAIVRAFQCGVMVLTGGPGTGKTTTLKLMIDLFE
                     GRGLRVMLAAPTGRAAKRLSEASGRQAKTIHRLLEFEPGEEGWRFARGVSSPLECDVL
                     IVDEMSMVDILLMNNMLKAVKPGTRLILVGDVDQLPSVGPGNVLRDIIASGVVEVVVL
                     DEVFRQAPGSMIVSNAHRINRGEFPHLRGARDFFFVDKEDPEE"
     assembly_gap    1999..2008
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2009..2638
                     /locus_tag="PLM83_07085"
     CDS             <2009..2638
                     /locus_tag="PLM83_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOK71203.1"
                     /translation="EVAQAVVDTVKSRLPRHLKCNPVDDIQVLSPMRRSVTGVDNLNV
                     LLRDALNPARQGVSELRYGATVFRRGDKVMQIRNNYERRVYNGDMGRIVWIDPEEGEV
                     VVRYPEAEGDRNVTYLQDDLDELVLSYCVSVHKSQGSEYTAVVIPVTTQHYVMLQRNL
                     IYTAVTRAKEAVVMIGTKRALAIGIKNASLLERRTMLAERLRDLAESTV"
     gene            2759..4534
                     /locus_tag="PLM83_07090"
     CDS             2759..4534
                     /locus_tag="PLM83_07090"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOK71204.1"
                     /translation="MFHSYLIFSEPKVWAGFGRSPEFEWMFLNTRSPTDLVVLTPPLP
                     VAIAELVARELNASIPIDAPPERALKRGRRIVKSVLSAVGYGGRSRLPRPMLLSASSF
                     DSSVHGLDLPPSDVLKACIDELGGRALLDEEAKRALADAGLCGLDRADHLLGAMVLDG
                     NATILPGVGITSIGGFKCNRCGSRGPFLQLPCARCGRDICAVCSECRNMGIVRECTPI
                     YLVNPPYVGTSIIGSVADKANMVMPHALTPAQVDAARALVEWYETDESPEAIVWAVCG
                     AGKTEVSYGLIAAALQAGKRVAYAAPRRDVVAELSPRIQSAFPAARCGVFYGGSSERY
                     FGADITLLTTHQAMRFYGVFDLVIIDEADAFPYYGSEALAASVDRSLAFGGRTVVLTA
                     TPSLGHIARIKAGTLPCFKISARHHGHPLPMPVVIEMGDKPQEPVVAELATASLERGC
                     PVLVFVPTRHRAPEVAEYLSQTVPDARTKWVHSGDAMRDSKRRMLAEGECEVLVSTSV
                     MERGITVDGVDVIVADADWETVFDCRALVQMAGRVGRTASNPTGSVWFTCSRENRQIQ
                     AAREMIDEMNRDAASRGFLNAGSEV"
BASE COUNT         1023 a         1087 c         1437 g         1049 t
ORIGIN      
        1 cgctctgtca ggcaaggacc ccaccaaggt ggacaggtct gcagcttatg ccgcgaggca
       61 tgtggcaaag aacctcgttg ccgcgggtgt ggccgacaaa tgtgaggttc agattgccta
      121 tgcgatagga gtcgcccgcc ctgtatcgat ttcggtcgac acttatggaa cgggcgctat
      181 cccagatgaa cggataacca ggatagtgcg tgaggtcttt gaccttcgac ctgaggcgat
      241 aatacgcaaa ttcgatctgc gtcgccctat atacaggcag gtttccgtat atggccactt
      301 cggaaggccc gacctcgatc tgccttggga ggcgttggac atggttgaag aggtgcgtaa
      361 gcgcatgtaa ggcatgcgcc tgcaatattc ggtcacgact ggggggcatg agcgctcccc
      421 agtcgtggta ttggcaccgt ggtgaggtga acgaagctgg atcttgtaga gggaatcatc
      481 gacagggtga ccttccgcga cgaggatact ctttatactg tggcccgcat tagcacggat
      541 agcggaatca tgacggccgt cggtccattc agctcagcca cgcctggcca agctgtgaag
      601 ttgactggag actgggttgt tcacaggtca tatgggcgcc agttccgagt cgcgaagatg
      661 gaagtagttg cgccggctac tgttgagggg atagagaaat atcttggctc cgggttgatc
      721 cccggcatag gacctgtaac cgcgaagaga atcgtagagc attttgccat ggagacgtta
      781 gaagtcatcc agtcctgtcc tgacgccctg ttgaaagtgc cgggtattgg cccgaaacgt
      841 gcagcgcaga ttgtgaaagc cgtggaagag cataagtcta ttgagaaagt gatggtcttc
      901 ctacagtcgc acggggtgag ccccacgtat gctgcgaaga tttacaagcg ttacggagac
      961 gatgctgtag cgattgtcag tagaaaccct tatcaacttg ccgatgacat ttacgggata
     1021 ggttttaaga ccgccgataa gatagcgagg gaggtcgggg tcgacaagga ttcaccatac
     1081 cgcgtcaact cgggaatcaa gtactggctg gccaaggcgt cagatgatgg ccattgctac
     1141 tcggtggcgg aggatgttat acgtgaggtc gcagccgagc tggacgtttc ggaggacttg
     1201 gttaagcagg gccttggggc cctggaggaa tcgggcgagg tattctgcga gcgcgaatcg
     1261 tcggtatggc tgggcccatt ctatcattct gagcgtggtg ttgcgaaacg cctttgggaa
     1321 ctgaatcatg gcaggatgtg gcagttagat gcgctatccg atgaagaact cggtcgggct
     1381 caggctgagg cgggattaac ccttggagca cagcaaaagc aggcaatagt ccgggctttt
     1441 caatgtggag tcatggttct taccgggggc cctggaaccg gaaagactac taccctaaag
     1501 ctgatgattg accttttcga aggtagggga ctccgtgtga tgctggcggc gcctactgga
     1561 agggctgcga agaggctttc ggaagcgagc gggagacagg cgaagactat acataggctt
     1621 ttggagtttg aacccggcga ggagggatgg cgttttgcac ggggggtatc gagcccgctc
     1681 gagtgtgatg tgctgattgt ggatgaaatg tctatggtgg atattcttct catgaacaac
     1741 atgctgaagg ctgttaaacc tggaactagg ctgattctcg ttggcgatgt cgatcagctt
     1801 ccatctgtgg gccctggaaa cgtgttgcga gacatcattg cctccggcgt ggtggaggtc
     1861 gtcgtcttgg atgaggtttt caggcaagct ccaggaagca tgatcgtttc aaatgcgcac
     1921 cgaataaacc ggggcgaatt tccccacctc agaggcgcac gggacttctt ctttgtggac
     1981 aaggaagatc ctgaagagnn nnnnnnnnga ggtggctcag gccgtcgttg atacggttaa
     2041 atcgaggctt cccaggcatc tcaagtgtaa ccctgttgat gacattcagg ttctctcgcc
     2101 tatgcggaga agcgtcaccg gcgtcgacaa tctaaatgta ctcctgcgag atgctctgaa
     2161 ccctgcgagg caaggggtat ccgagctaag atacggggct acggttttca ggcgtggtga
     2221 caaggtaatg cagattcgca acaactatga gcgaagggtg tataacggtg atatgggccg
     2281 gatcgtgtgg atcgatccgg aggaaggcga ggttgtagtc agatatcctg aagctgaagg
     2341 cgaccgtaat gtcacgtatc tccaagatga cctggacgaa ctagtccttt cctactgcgt
     2401 gtcagtgcac aaaagtcagg ggagcgaata cacggcggtg gtcattcctg tcaccactca
     2461 gcactacgtt atgctgcaac ggaatctcat ctatactgcg gtcaccagag cgaaggaagc
     2521 tgtggttatg ataggaacca agagggcgct tgcaatcggc ataaagaatg catccctgct
     2581 agaaagaagg acaatgctgg ctgagagact cagagatctg gcagagtcaa cagtatgaga
     2641 aggagacatg ctagcaacag atggtgttag ccaggatgat tttgccgctt aatggaggaa
     2701 aatggtggcc catgcagaag ctaaatggca tcatgacctc cagaggtggg ccgacgccat
     2761 gttccattcc tacctgatat tctccgagcc caaggtttgg gctggattcg ggcgttcgcc
     2821 cgagttcgaa tggatgttct tgaacacgcg atccccgacc gacctagtag ttctcacgcc
     2881 tcccctgcct gtagccattg cagagcttgt ggcgcgcgag ctgaatgcat cgattcccat
     2941 tgatgccccg cccgagcggg cattgaaacg tgggcgccgc atagtcaagt ctgttctatc
     3001 agctgtcgga tacggcggtc ggtctcgtct tccgcggccg atgctcctgt cggcgtcttc
     3061 atttgattcg tctgttcatg gattagactt gccgccgtct gatgtcttga aggcatgcat
     3121 agacgaactg ggaggccgtg cgcttttaga cgaggaggcc aaacgggctc tggctgatgc
     3181 aggtctgtgc ggtctggacc gtgcggacca tctgctgggt gccatggttt tggacggcaa
     3241 tgccacaatc ctcccaggag tgggcataac cagcatcggc ggcttcaaat gcaacagatg
     3301 cggttccaga gggccttttc ttcaacttcc atgtgccaga tgcggcagag acatttgtgc
     3361 agtctgttcg gagtgcagga atatgggaat tgttagagag tgtacgccta tctatcttgt
     3421 caacccgcct tacgttggca cctcaatcat cgggtcagtt gccgacaagg cgaatatggt
     3481 aatgccgcac gcgcttactc ctgctcaggt agatgctgct cgggcccttg tggaatggta
     3541 cgagacggac gaatcccctg aggctattgt gtgggcagta tgtggcgcag gaaaaaccga
     3601 agtctcgtac ggcctaatcg ctgctgccct gcaggcaggc aagcgtgtgg catacgcggc
     3661 gccgcgcaga gacgtagtgg cagagctgtc tccgcgcata cagtcggcct ttccagcggc
     3721 tcggtgcggc gtcttctatg gaggctcttc agaacggtat ttcggagccg acataactct
     3781 tctcaccacc caccaggcga tgcgatttta cggagttttc gacctagtga taatagatga
     3841 agcggatgcg ttcccatatt acggctctga agcgcttgcc gcttcggtcg acaggtcatt
     3901 ggcctttggc ggacgtacgg tggtgctcac ggccacgccg tctttagggc acatagccag
     3961 aataaaggcg ggtactctgc cctgtttcaa gatatcggcg cgtcatcatg gtcatccttt
     4021 gccaatgcct gtggtgatcg agatggggga caagccccag gaacctgtcg ttgccgaact
     4081 tgctacagct agtctcgaac gaggatgccc ggtcttggtt ttcgttccca cacgccatcg
     4141 tgccccagaa gtggcggaat atctatctca aactgtgcca gatgcgagga caaagtgggt
     4201 ccattcaggc gatgcaatgc gcgacagcaa aaggagaatg ttggcagaag gtgaatgtga
     4261 agttctggtc agcacatcgg tgatggaaag gggcattaca gtagacgggg tcgatgtaat
     4321 agtggctgac gcggattggg agactgtctt cgactgcagg gcgcttgtgc agatggctgg
     4381 gcgggtaggg aggacggcat cgaatccgac agggagtgtg tggttcacgt gctcaaggga
     4441 aaaccggcag attcaggctg cgcgggagat gattgacgaa atgaaccggg atgcggcgtc
     4501 ccggggcttc ttaaatgctg ggagtgaggt atgaagcatg attcagctgt cacgctttcg
     4561 ggattttgtg gacacagtgc tgttcggaag ggagcttcgt tgcgcg
//
LOCUS       DAOSAI010000192         4596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11852_length_4596_cov_4.393526, whole genome shotgun sequence.
ACCESSION   DAOSAI010000192 DAOSAI010000000
VERSION     DAOSAI010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11852_length_4596_cov_4.393526"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..762
                     /gene="pcp"
                     /locus_tag="PLM83_07095"
     CDS             139..762
                     /gene="pcp"
                     /locus_tag="PLM83_07095"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003353092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HOK71205.1"
                     /translation="MARILITGFDAFGGEEVNPASRAVQHLGASGVWKQGGVEVITKE
                     IPTVYGASARAVQNAICELSPQAVISVGQAGGSTGIRVERVALNLDDARIPDNSGNQP
                     SDRLIEPEGPVAYWATIPVRGVVEAVRAKGIPAFLSYSAGTFVCNHVFYSTCHFVASN
                     NLPIKVGFMHVPYLPEQAVGKTETPSMSEECIVAALEAAVDAVAASL"
     gene            1419..3527
                     /locus_tag="PLM83_07100"
     CDS             1419..3527
                     /locus_tag="PLM83_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOK71206.1"
                     /translation="MKGLAEFVVKNRVAILIIAAVLLVPSVYGMQHRKVQYDLLTYLP
                     KDLNSVEGMNVLDEEFKYAERAFVVCRNQPDWAVLKVKEKLEHVDGVSSVFWLSDLVD
                     PTIPHDYLGEDIVSKFYRGQITLLIVNFEEDAVSPRTERAVNDIKNLLDKEQWVIGAL
                     VSGLELKDLGVEQTPVMVRTAVLLILILLFLTLPSPLMPLVFMGTIGVAYAYNMGINY
                     LLGRDVSYVTNAVASAMQLGVTMDYCIFLLHTFQTECEKHDSEEAMVRAITYTANAVL
                     SSATTTAVGFGSLALMRITLGADMGILMARGVLLSVICCLTILPSLVLTLNPILGKWN
                     HPVILPSYKGLGDWLTEQRVPVFALTVVLALVGLYGYNKIEPSYDIESTFPMDLPSIA
                     ALQEFKDDLGSVEEAYIITRGVPQWRLQELTRQIREMDGVKDASCLGDVVDPGIPVEF
                     VPEGVSKQYVGGEYENTVITLEGSILDPRTPEFITQTRELIKDLPGEVYFTGTSVIGK
                     DLEDLSSKDLDLVNWVSMAGIFAIVAIAFKSWTVPILLILAIKVAIWVNQAISYFTGA
                     PVFFFSRMAIEAIQLGATVDYAILVTNTFKGKLNKNAPPDAMRSTVAECGPAVLNSGL
                     AMFCAIIGVYASTNLRAIKDLALLLARGALVSVAVTSFMLPATLVVCNPILEKTSIGW
                     PKHDSVRQAGTSAVAASDDK"
     gene            3550..>4596
                     /locus_tag="PLM83_07105"
     CDS             3550..>4596
                     /locus_tag="PLM83_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71207.1"
                     /translation="MKNRTAKITAVIGACAILMLCSVGMAQSPGGVSETSGTSGTYGR
                     IQEYETVFCVLASDGMPESTTVVDWIRVPQGGPVSVFDPGNIENPVNLRNAEVPDVVS
                     GGLKWNADPTRITDINYSGTSKKQLPIGVEVTYKLNGKVVPAQAVPGSSGRLEVTVDL
                     VNRTARKENLSYTVDGKNTTFAEDVCVPMIVQVSTDVPLPDYTLIQAPDATTVLAGKS
                     LKINWTLMPNPDASCTLVLEGENIQLNDIDVTVIPSMPPIPQLEELLDTAIDALGTLA
                     SGVDQLDSAIEQAQSGTLKLADGQAQVADGMIAIGNGVSDLSKLADAHWTIAKTMNDA
                     ITPEMLEKPKQLIEL"
BASE COUNT         1020 a         1082 c         1349 g         1145 t
ORIGIN      
        1 aggcgaggtg ctgtgcatta tcgcaaagta ggtgcatgcc ggggcagtgt tcgggtggcc
       61 tcccgtggcg gcgtggttca ttgaggctaa aagggagtct gcaccggcta taatggacgc
      121 ctttgagggg gcttagctat ggccaggatt cttataacag ggtttgacgc ttttggaggc
      181 gaggaggtga atcccgcctc cagggcggtt caacatcttg gggcgtctgg agtgtggaag
      241 caaggcggcg ttgaggtgat aacaaaggaa atacctacgg tgtatggggc ttcagctagg
      301 gcagtgcaga atgcaatatg cgagctttcg ccccaggcgg ttataagcgt aggccaggcg
      361 ggcggctcga ccgggatacg cgttgagagg gtggccctta accttgatga tgccaggatt
      421 ccagacaatt caggaaatca gcctagtgac agacttattg agccggaggg gccagttgct
      481 tactgggcca ctattcccgt gcgaggagtt gtagaggctg tacgggccaa agggattccg
      541 gcattccttt catacagcgc tggaaccttc gtgtgcaatc acgtcttcta ctcgacctgc
      601 catttcgtcg cctcaaacaa tctgccgatc aaggtgggct tcatgcacgt tccatacctc
      661 cctgaacagg cagttggaaa gacggagact ccgagcatgt cggaggaatg cattgtggct
      721 gcattggagg cggcagttga cgcggtggcg gcgagtctgt gatctctgcc ctttgctggc
      781 aaatggccgg ctcggggcgt tgagcagcct ggtgatgtgg tggattcgtg atgaaatggc
      841 tttgtggttg ggggtgttct gcaacgcgaa tgttcggtat tgagtttttc attgccgtgg
      901 agacggtttg tgcacacgag ataatggtta gcctagggcg acgctgactc aaagtgcaat
      961 catcacatct gcgagtacga cctgctggcg tccacacgct cttgctttgc ttggtgtgtt
     1021 gtacgcgtat acgaaaaact tgcgtattct ctgggttggt tcctttcata taactatttc
     1081 cttccagaac tcaagttggc ttggcgcttg aattcttcct caatgctgta gattaggtta
     1141 tcggtgatat gttgtgtttt gtgattctct atggtttggt tgccttgggg aatcttgccc
     1201 tcggctacga agcccccgac catcaccggg aggctcctca attaccgtaa tctcccaccc
     1261 gtgtgcaagg tttcgtgcac aaaatccctg cattatgcgt atttgtaaat tcagaatatt
     1321 atagattgcc ccttgacatt aatgccataa gcgactagga tatcgttgta ctgaccggtc
     1381 agtataaaaa ggcgttgtat ggaagtaggg actcatcagt gaaaggctta gctgaatttg
     1441 tcgtaaaaaa cagagtggcg atactgatca tcgctgccgt gctgttggtt ccgtcggtct
     1501 acgggatgca gcacaggaag gtacagtacg acttgctcac gtatcttccc aaagacctca
     1561 attccgttga ggggatgaac gtcctcgatg aggaattcaa gtatgcggag cgagctttcg
     1621 tagtctgtcg gaatcagcct gattgggcag tgctgaaggt gaaggagaaa ctcgagcatg
     1681 ttgacggagt ttcctcggtc ttctggcttt ctgacctagt cgatccgacg ataccccatg
     1741 attatttggg cgaggatatc gtctctaagt tctacagggg ccagatcaca ctcctcattg
     1801 tcaatttcga ggaggatgcg gtttcgcccc gaactgaacg tgcggtcaat gacatcaaga
     1861 acctgcttga caaggaacaa tgggttatag gtgcgctcgt cagcggtctg gagctaaagg
     1921 acttgggggt tgaacagacg cctgtcatgg tccggactgc ggtcctgctc atattgatcc
     1981 tcttgttctt gaccctgccg tcgcccttga tgccgctggt cttcatggga accataggcg
     2041 tggcctatgc ttacaacatg gggatcaact acctgctagg gcgtgacgtg tcgtatgtga
     2101 ccaatgcggt ggcttcggct atgcaactcg gtgttaccat ggactattgt atttttctac
     2161 ttcacacatt ccaaaccgag tgcgagaagc atgactctga agaagccatg gtgcgggcga
     2221 taacctatac tgccaacgcg gtattgtcaa gcgcaaccac gactgctgtg ggctttggat
     2281 ctttggcgct gatgcgtatt accctcggag cagatatggg gatactaatg gcacgcggcg
     2341 tgctgttgtc ggttatatgc tgtctgacca tccttccaag cttggtgttg accctcaatc
     2401 caatcttggg aaagtggaat catccggtca ttcttccaag ctacaaaggg ttgggagact
     2461 ggctcactga gcagagggtt ccggtatttg ccctcaccgt agttctggcg ttggttgggt
     2521 tgtacggata caacaagatt gagccgagct acgatataga gagcacgttc ccgatggatc
     2581 tgccttccat tgcggccctc caggagttca aagacgacct tggcagtgtg gaggaggcat
     2641 acataataac tcgtggcgtg cctcagtgga ggctgcaaga actcacgcgc caaatccgag
     2701 agatggacgg ggtgaaagac gcttcgtgtc ttggagacgt ggtcgacccc gggattcccg
     2761 tggagttcgt gcctgaaggg gtatccaagc agtacgtggg cggcgagtat gaaaacaccg
     2821 tgatcactct cgaaggctca attctcgatc ctagaacgcc ggagttcata acgcaaacga
     2881 gggagcttat caaggatttg cctggagagg tctattttac cgggacttca gtgataggca
     2941 aggatcttga agatctcagc tccaaggacc ttgatttggt taactgggtt tcaatggcgg
     3001 gcatctttgc catagtggcg atagccttca agtcatggac ggtgccaatt ctattgatac
     3061 tcgctattaa ggtggcaatc tgggttaacc aggcaatatc gtatttcacc ggagcgccgg
     3121 tcttcttctt ctccaggatg gcgattgagg cgatacagct cggcgctact gtggactatg
     3181 cgatcttggt taccaataca tttaagggaa agctgaacaa gaatgcgccg cctgatgcga
     3241 tgcggtcgac agtggcagaa tgcgggcctg cggttctcaa cagcggcttg gcaatgttct
     3301 gcgccataat cggagtctac gcatccacca atctgagggc catcaaggac cttgctctct
     3361 tgctggcgag gggtgctttg gtgagcgtgg cggttacatc tttcatgctc cctgccacgc
     3421 tggttgtgtg caatccgatc cttgagaaga cctctatcgg atggcccaag catgactctg
     3481 tgagacaggc ggggacgtcg gctgttgcgg cgtccgacga caaatgatca ttttacgagg
     3541 tgacttacgt tgaagaatag gactgccaag atcacggctg taataggcgc ctgtgcgatc
     3601 ctcatgctct gcagtgtggg catggcacag tcgccgggag gagtctcgga gacttcgggc
     3661 acttcaggga cttatggaag aatacaggag tatgagacgg tcttttgcgt cctggcgtct
     3721 gatggcatgc ccgagtccac taccgtagtg gactggatca gagttccgca gggtggcccg
     3781 gtatctgttt ttgaccctgg gaatattgag aatcctgtaa acctcaggaa tgctgaggtt
     3841 ccggacgttg tttccggcgg gctcaagtgg aatgctgacc ccactcggat aacggacata
     3901 aactactccg gaacttcgaa aaaacagctt ccgatcgggg tagaggttac ctacaagctt
     3961 aacggcaagg tcgttccggc tcaggctgtt ccgggcagtt ccggcaggct ggaagtgact
     4021 gtggatcttg taaacaggac agcgaggaaa gagaatctga gctatacggt tgatggcaag
     4081 aatactacat ttgccgaaga tgtttgcgtt ccaatgatag tgcaggtatc gacggatgtg
     4141 cctcttccag actacacgct tatacaggcg cctgacgcca ctacggttct ggctggcaag
     4201 agcctcaaga tcaactggac gttgatgccc aacccggatg cctcgtgcac gttggttctg
     4261 gaaggcgaga atatccagct gaacgatatt gatgtcaccg tcataccttc gatgcctcca
     4321 attcctcagc tcgaagagtt gctggatacg gcgatcgacg cgcttgggac gttggcgtcc
     4381 ggagttgatc agctcgattc cgccattgaa caggctcagt cgggcacgct gaagcttgct
     4441 gacgggcagg ctcaggtagc ggacggcatg attgcaatcg gaaatggggt gtctgacctc
     4501 agcaagctgg cagatgccca ctggacgatc gcaaagacga tgaatgacgc tattaccccg
     4561 gagatgctcg aaaaacccaa acagctgata gaattg
//
LOCUS       DAOSAI010000193         4596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11856_length_4596_cov_3.248844, whole genome shotgun sequence.
ACCESSION   DAOSAI010000193 DAOSAI010000000
VERSION     DAOSAI010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11856_length_4596_cov_3.248844"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PLM83_07110"
     CDS             <1..1188
                     /locus_tag="PLM83_07110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK71208.1"
                     /translation="IVGLGPAAVNALEGIRSQDVDVPVTILSQEPYYAYYRPRLSHLL
                     GEEVSSESLAIKKPQWYEERNAQVLLGQTAKTINVDRKTVSLDNGSELSASSVILACG
                     SSPFVPPLSGLDRENVFSLRTVDDARAISSACETAKTVCVVGAGPLGLEAAWALAKRD
                     LEVHLLEHGSRLLSKMLDQNASRMLAEIVESAGIVLHLSAFSEALFPGGIRLAGGEEI
                     PGEVILFSTGVRPNLDVPRSAGLKINRGVEVDKLMRTSTPWIYAAGDVAEFEGSVGGI
                     LPVAVAQGKVAGVNAAGGQEEYVAVPPSYTLMVMGTRLFSVGDVQDGPDVTSIVDVDY
                     DSRVYRKLVLSGDKVVGGILFGDIAGSDKIRRAVEEGVQLPGAAKESVPDYASLLDMI
                     VRD"
     gene            1222..2694
                     /gene="miaB"
                     /locus_tag="PLM83_07115"
     CDS             1222..2694
                     /gene="miaB"
                     /locus_tag="PLM83_07115"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOK71209.1"
                     /translation="MEVRRRSAKVVTFGCQMNERDSETIMGILSSLGFEESSRVEDAD
                     IVIFVTCCVRENAENRAFGNLGEMKRLKEGNPNLVVGMCGCMAQRSDLREIVRARMPW
                     VNLVFGTHNIHRLPELLERVFAGERVVEVWDEPDEVVEGLPAVRAYGHKAFVNITYGC
                     DNFCTYCIVPYVRGRERSRRMEDVVAEVCDLAGSGCVEVTLLGQNVNSYGRGLPPLPS
                     GSSPDFADLLHEVNLVDGIRRIRFTTSHPRDFSDKMIEAVASSEKVCEHFHLPLQAGS
                     DRVLEMMHRGYTAERYLALLDRIREAVPGASITTDIMVGFPGETEEDFQATMDVVRQA
                     RFDAAFTFIYSPRPGTVAARMPDQIEDEVKHDRVYRLIDLVNSIASEVNDELCGSIVE
                     VMVEGPSERDPSVLSGRTRTNKMIHWTSEKAHNPGDIVMLRVSQTRTWTLYGEEIEGR
                     SGHRGALTCDGHEAAGEEMQGRTASSSGLATDGRCDCNDV"
     gene            2687..>4596
                     /gene="mutS"
                     /locus_tag="PLM83_07120"
     CDS             2687..>4596
                     /gene="mutS"
                     /locus_tag="PLM83_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOK71210.1"
                     /translation="MSKPTPMIRQYRELKKRCPDAILMFRLGDFYEMFMEDAEIASRI
                     LGIMLTSREVGSGNKMPMCGVPHHAVDQYIATLVQNGHKVAIADQVEDPRTAKGLVRR
                     DIVRVVTPGTLADQVDPGRNNFLIAVVATESGVGLASCDASTGELAATQADGDDAFAL
                     ISEEIDRIAPAECIYPGDSRGLQGSSGPRFDEDDKGGGCPVEGSFVAYPRPPMDFERG
                     RAEETLMSQFGVTSLEGYGAAGMDLAVRAAGALVSYLQETQMTSVPQISRMRVYHLGE
                     FMRMDTPTRRALEIFHRERGLLSVLDSTVTPMGGRLIRSWFERPAVSAAEIESRLDAV
                     EELAGDLFLRADVRKTLDRMYDLERTCGRIGYRTAGPRDLLALASSLEAVDDLAAVLG
                     EAKSSRIIDLAGALDPVPEATERIRASIKLDAKASANEGGVICEGYSEEVDELRRAST
                     EGKEWLIRLEARERERTGIKSLKVGFNQVFGYYIEVTHANSHLVPDDYTRKQTLANSE
                     RYITPELKELESKILGAEERLIRLEAAIFEQVRDDISAYLPRILATAQAVAELDVLAS
                     FAEDARNRGYVRPQINTGKATVIREARHPVLEEMLGSARYVPNDIEASCNGGRLLIIT
                     GPNMAGKSTVLAT"
BASE COUNT         1007 a         1088 c         1474 g         1027 t
ORIGIN      
        1 atagtaggat tggggcctgc tgcggtcaat gcgttggaag ggataaggtc gcaggatgtg
       61 gacgtgccgg ttacaatatt gtctcaggaa ccatactacg cttattatcg tcctcggctt
      121 tcacatcttc ttggtgaaga agtgtcctct gaaagcttgg caattaagaa gccgcaatgg
      181 tatgaggagc gcaatgccca ggtattgctg ggtcagacag cgaagacgat caatgttgat
      241 cgcaaaaccg tgtcgctgga taatggcagc gagctttccg cttcctctgt gatactggcc
      301 tgtggaagca gtccgttcgt gcccccgctt tcggggcttg atagggagaa tgtgttctcc
      361 ttacgtaccg tcgatgatgc gcgggccatc tcctcagcct gtgagaccgc caagacagtt
      421 tgcgtcgtgg gcgcaggtcc cctggggctt gaggctgcgt gggccctggc caagcgcgat
      481 ctcgaggttc atctgttgga gcatggctct cgtttgctct cgaagatgct tgatcaaaat
      541 gcatcacgga tgctggcgga gatcgtggaa tccgccggaa ttgtccttca tctatcggct
      601 tttagcgagg ctctgtttcc gggcgggatc cgccttgcag gaggcgagga gattcccgga
      661 gaagtaattc tgttctccac aggcgtaagg ccgaatctcg atgttcctcg ttccgctggc
      721 ctcaagatca atcgcggggt ggaggtcgac aaattgatgc gcacatcgac gccgtggata
      781 tacgctgccg gcgatgttgc tgaattcgaa ggctctgtag ggggaatcct gccggtggcg
      841 gtagcgcagg gcaaggtagc tggcgtcaat gccgcgggtg gccaagaaga gtatgtggcg
      901 gttccgccct catatacgct gatggtgatg ggaacaaggc tcttttccgt tggcgatgtg
      961 caggatggtc ccgacgtaac gtccatagta gatgtcgatt acgatagccg agtgtaccgc
     1021 aaactcgtgc tgtcaggtga caaggttgta ggcggcatcc tctttggcga catagccgga
     1081 tccgacaaga ttcgccgggc tgtagaagag ggcgtccaat tgcctggcgc tgcaaaggaa
     1141 agcgtgcctg actacgcttc tcttctggac atgattgtga gagactagga aatctacata
     1201 gctttttatg gaggcggcgg tatggaagtc agacgtagat cagccaaggt ggtaactttt
     1261 gggtgccaga tgaatgaacg cgactctgag acgattatgg gcatcctgtc aagcctcggc
     1321 ttcgaagagt cttctagagt tgaggacgcc gacattgtaa tattcgtcac ctgctgtgtt
     1381 agggagaatg ctgagaaccg cgcttttggc aatttgggag agatgaagcg cttgaaagaa
     1441 ggcaacccta atctcgtggt tggcatgtgc ggatgcatgg cccaaagaag cgatctcagg
     1501 gagatagtgc gcgctcgaat gccctgggtt aacctggtgt ttggcactca taatatccac
     1561 aggctgcctg agcttctcga gcgcgtcttc gccggcgagc gggttgtgga ggtgtgggac
     1621 gagcctgatg aagtagttga aggtcttcct gctgtgcggg cctatggcca caaagccttc
     1681 gtcaatatca cgtacggctg cgacaacttc tgtacatact gcattgtgcc gtatgtgagg
     1741 ggaagagagc gtagccgccg catggaagat gtagtagctg aggtctgcga tctagcgggg
     1801 agcgggtgcg tcgaggtgac gctattgggt cagaatgtca actcttacgg tcgtggcctg
     1861 ccgccgcttc cgtcggggtc atcgcctgat ttcgctgacc ttctacacga agtcaacttg
     1921 gtggatggca ttagacgcat caggtttacc acatcacacc ctagggactt ttctgacaag
     1981 atgatagagg ctgttgcctc atcggagaag gtgtgcgaac attttcactt acctctgcag
     2041 gcagggagcg atagagtgct tgagatgatg cacagaggat atactgccga gaggtatctg
     2101 gcgcttttgg atcggataag agaagctgtt cccggggcat ccataacgac agacataatg
     2161 gttggttttc cgggagagac ggaggaagat ttccaggcta cgatggatgt ggtgaggcaa
     2221 gcgagattcg atgcggcatt tacgttcatc tattctccca ggccagggac ggttgcggct
     2281 cgaatgccag accagattga agatgaagtg aagcatgacc gcgtctatcg cctgatagac
     2341 ctcgtaaact cgatagctag cgaggtcaat gacgagctgt gtggatcgat agtcgaggta
     2401 atggtggaag ggccgtccga acgcgacccg tcggtccttt cagggagaac aagaacgaac
     2461 aagatgatcc attggacttc tgagaaagct cacaatcctg gcgacattgt catgctgcgc
     2521 gtttcacaaa cgcgcacttg gacgctgtac ggcgaggaaa ttgagggtcg tagtgggcat
     2581 cggggtgcgc taacctgtga tgggcacgag gctgccggag aggagatgca aggccgaaca
     2641 gcgagctcct caggcttggc tacggatgga aggtgtgact gcaacgatgt ctaagccgac
     2701 tcccatgatc cgccaatacc gagagttgaa gaagagatgt ccggatgcca ttctcatgtt
     2761 caggctgggc gatttctacg agatgttcat ggaagatgcg gagatcgcca gcagaatatt
     2821 ggggataatg ttgacctcac gcgaggtcgg ctcgggcaat aagatgccca tgtgcggggt
     2881 tccgcatcat gctgttgatc agtacattgc cacgctggta cagaatgggc acaaggtagc
     2941 cattgcggac caggtggaag acccgcgcac tgccaagggg ttggtgcgca gggacatagt
     3001 gcgggtcgtg actccaggga cgcttgcgga ccaagtggac cccggccgca acaattttct
     3061 catcgccgtt gttgcgaccg agtcaggcgt ggggctcgct tcgtgcgatg cgtccactgg
     3121 tgagctcgcg gctacgcagg cagacggcga tgatgcgttt gcattgattt cagaggaaat
     3181 cgacaggata gctcctgccg aatgtatcta tcctggtgat tcacgtgggc ttcaaggttc
     3241 gtctggccct cgttttgatg aggatgataa ggggggcggt tgcccggttg agggtagttt
     3301 tgtcgcctat cccaggccgc caatggattt cgaacgtggg agggctgagg agacgctaat
     3361 gtcccagttt ggggtgacct ctctcgaagg ttacggcgca gcaggaatgg atcttgctgt
     3421 cagggccgcc ggcgccctcg tatcctactt gcaggagacg cagatgacca gcgtgccgca
     3481 gatttcaagg atgcgcgttt accatctggg cgaattcatg aggatggata cgccgacccg
     3541 gcgcgcgttg gagatattcc accgcgaaag gggtcttctt tccgtcctcg atagcactgt
     3601 gacgcccatg ggcgggcgtc tcatcaggtc gtggttcgaa cgccctgctg tgtcggcggc
     3661 tgaaatcgag agccggttgg atgccgtgga agagctcgca ggtgatttgt ttctcagggc
     3721 agatgttcgc aagacattgg atcgcatgta cgatctggag cgcacatgcg ggcggatagg
     3781 gtataggacg gctgggcccc gggatctttt ggcgctggcc tcgtctcttg aggccgtgga
     3841 tgacctggcc gctgttcttg gtgaggcgaa gtcttccagg ataattgacc tagcgggggc
     3901 gcttgatccg gttcctgaag caacggaacg tataagggca tctatcaagc ttgacgcgaa
     3961 ggcaagcgct aacgagggcg gagtgatctg tgaaggctac agcgaggagg tagatgagct
     4021 caggcgtgcc tccacggaag ggaaggaatg gctcataagg cttgaagccc gtgagcggga
     4081 acgcactggc ataaagtcgc taaaagtcgg cttcaaccag gtgtttggat actacatcga
     4141 ggtcactcac gcaaacagcc acctggtgcc ggatgattac actcgcaagc aaacgctggc
     4201 caactccgag cggtacatta cgccggagct gaaggagctg gaatcgaaga tcctcggtgc
     4261 agaggagagg cttatcaggc tcgaggctgc gatattcgag caggtcaggg acgatatcag
     4321 cgcttacctc ccgaggatac tcgcaacggc gcaggcagtg gcggaactag atgtgctggc
     4381 ttcatttgcg gaggatgcgc ggaaccgagg ttacgtaagg cctcaaatca acaccgggaa
     4441 agccacggtg atacgcgagg cgagacaccc cgtgctcgaa gagatgttgg gatcggctag
     4501 atacgtgccc aacgacatag aggcttcgtg caatggtgga aggttgctga tcataacagg
     4561 tcccaacatg gccggaaaaa gtacggtgct tgccac
//
LOCUS       DAOSAI010000194         4577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11910_length_4577_cov_4.201902, whole genome shotgun sequence.
ACCESSION   DAOSAI010000194 DAOSAI010000000
VERSION     DAOSAI010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11910_length_4577_cov_4.201902"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLM83_07125"
     CDS             complement(<1..448)
                     /locus_tag="PLM83_07125"
                     /inference="COORDINATES: protein motif:HMM:NF028512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOK71211.1"
                     /translation="MKLGRFLLLQDRVFLFAIVVIVFAIATAGAVSIWTMKSELENRS
                     AECAVSIARTFALATPVRENLGKPGGASVIQPMAEAVSCASGAQFVVTIDMEGIRYSH
                     PIPDRIGKHVAGGDEDRSLAGEEYVSRACGTMGPSMRAFVPIYDPSG"
     gene            799..2178
                     /locus_tag="PLM83_07130"
     CDS             799..2178
                     /locus_tag="PLM83_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HOK71212.1"
                     /translation="MSKQEIKILSATGIVGYGFSEDSLRRGMERKPDVLASDAGSTDP
                     GPFYLGSGTAFVSREACKRDASLLINAARDANIPLIIGTAGGAGGDENLQWFLDIVME
                     ILRENDLHLKLAVIHSEQDKSYIRSKMKEGKVKSMGALPELTDEEIDAAEHIVGLMGA
                     EPFIAAVELGADIVITGRSSDTSIFASMPIKQGFDPGLAWHAAKLLECGAMSAEPANA
                     GDCILVTMRRDHFLVEPLNPAQRHTTVSTAAHALYENASPYHLYEAGGMLDLSDAKYE
                     QVDERTVRVSGSKFIPDKTYRIKLEAATKMGYRTISIMGTRDPILIDQIDDYLANVRN
                     TVQRRIDDAFKGEVKQSDYRLGFRVYGKNAIMGEWDPNKNTTPNELGIVIDVVADTPE
                     LSKVILAFSRTFTLHHDFPGRKCIAGNMAVAFSPSDIPMGPVYRFSMEHLIEPNDPYE
                     MFRTEIFDV"
     gene            2202..2510
                     /locus_tag="PLM83_07135"
     CDS             2202..2510
                     /locus_tag="PLM83_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HOK71213.1"
                     /translation="MKLKDLAKVIRSKNAGPFYVTLDVMFSDAETYQKVKATGTLNAD
                     LIASLYNVAPDKVRFFEYDPAYSFKATIPRRIVSGDFGDTDSYGAQQHAPLLDVDISL
                     "
     gene            2548..3840
                     /locus_tag="PLM83_07140"
     CDS             2548..3840
                     /locus_tag="PLM83_07140"
                     /inference="COORDINATES: protein motif:HMM:NF015355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HOK71214.1"
                     /translation="MCQNQTRILGMEIKYYLVALAICIIGMAFDLVGNGMIFAIPMLL
                     VLSHGLTKIGDNIPIWKDWVGGGVTLTMFAGAILVKFIPETALESIKNFYVVDDFLVF
                     TIGNLLICSIMGMDRKVLTQAAVGFLPTVLAGVACAYLCGGLIGALLGYGFWDAILYI
                     SQPIMGGGMGAGAIPISKIYASAGLKDADAYLSMLVAAVVLGNIISIIVAAVLNKVGE
                     LRPELTGNGQLVKSAKSATQSESKVAEAQSVHLTPESIGMIIVWIGVFIILGNLARKI
                     IPVNIHPYAWMIILACAAKILNLFPEKFAQTARTYLPKYVALAMPPIMLGVGLFYTDL
                     EQFAASVSNPTFVLICLATVLGAVLGAGLFGRLFKFNFVESSMAAGLCMANMGGSGDL
                     AVLSAGKRMGLMPFAQISSRLGGAIVMIIAGAMLSLLR"
BASE COUNT         1137 a         1202 c         1189 g         1049 t
ORIGIN      
        1 taccggacgg atcatagatg ggcacgaacg cgcgcatgga tgggcccata gttccgcagg
       61 ctctcgaaac atattcttca cctgcaagag atcgatcctc atctccgccc gcaacatgct
      121 tgccgatacg gtcagggata gggtgcgagt atcgaatgcc ctccatgtca attgtgacca
      181 cgaattgggc accggaagcg cagctcacag cctcagccat aggttggatt acagatgctc
      241 cgccaggttt gccaagattc tcgcggaccg gcgtagccag cgcgaaggtc cttgctattg
      301 agacggcgca ctccgctgag cggttttcca attcagactt cattgtccag atcgacacgg
      361 ctccggcagt agctatggca aaaacgatga ctactatggc aaatagaaat accctgtcct
      421 gaagaagcag gaatcttcct agcttcatct ccaacccagc ccttcttcga tgcataccag
      481 caagaaacaa ccctattgtc tcactcgcgc ctctgatgtg gcaactgcca catcgcggga
      541 tgaagaagca atgctgtcca ttcttggcat atcccctaca gctcgagcag gatgatgcct
      601 atataacccc acaataagcc tactggcacg atcgtgcgct gccaagtttc tgcaagtatt
      661 taaaggcctt tcgaaactaa tgaacacggt tagcagggca gtctgtgtgc tagatttgaa
      721 ctggtgggtt gtctattcat gcaagaaaac ctcacctgta ttcaacacag cttctactat
      781 gttacggagg tgttccagtt gtcaaaacag gagatcaaaa tcctctctgc cacaggcatc
      841 gtaggatacg gattctctga agactcgctc cggcggggaa tggaacgaaa gccagacgtg
      901 ctggcatccg atgcaggttc aacagaccct gggccattct acctgggttc tggaacagca
      961 tttgtgtcgc gcgaggcatg caaacgagat gcttcgttgc tcataaatgc tgcacgcgac
     1021 gccaacatcc cgctgatcat tggaacggca ggcggcgccg gaggagacga aaacctccaa
     1081 tggttcctcg acatcgtaat ggagatactg cgtgaaaatg atctccatct taaactggca
     1141 gtaatccatt ctgaacagga caagtcctac atccgctcca agatgaaaga gggaaaggtc
     1201 aagtccatgg gagccctgcc agaactgaca gatgaggaga tcgacgcagc tgagcatatc
     1261 gtcggcctca tgggcgcaga accattcatc gctgcagttg agctaggcgc cgacatcgtg
     1321 atcacagggc ggtcaagtga cacatcgatc ttcgcttcga tgcccatcaa gcaaggcttc
     1381 gacccaggtc tcgcatggca cgcggccaag cttcttgagt gcggagcaat gtctgccgag
     1441 ccggcaaatg ccggagattg catactggtc accatgcgcc gtgatcactt cctagtcgaa
     1501 ccgctcaacc cggcccagcg ccacaccacc gtcagcacag ctgcacacgc cctatacgag
     1561 aatgccagcc catatcactt gtatgaagcc ggcggcatgc tcgacctgag tgatgcaaag
     1621 tacgagcagg tggatgagag aacggtccgg gtgtcgggca gcaagttcat ccctgacaag
     1681 acttatcgca tcaaacttga agctgcaacc aagatgggct acaggaccat atccatcatg
     1741 ggaacgcggg acccgattct tatagatcag atcgacgact atcttgcgaa cgtcagaaac
     1801 acggttcaaa ggcgcatcga cgacgcattc aagggcgaag tcaagcagtc tgactaccgt
     1861 cttggtttcc gtgtgtatgg caagaacgcc attatgggcg aatgggatcc gaacaagaac
     1921 acaaccccca acgaactggg aatagtcatt gacgtagtcg cggatacgcc cgagttgtcg
     1981 aaagtaatac ttgctttctc ccgaacattc accctgcatc atgatttccc aggccgaaag
     2041 tgcatagccg gcaacatggc cgtcgcattc tcgccctctg acatcccaat gggacctgta
     2101 tacagattca gcatggagca tctgattgag cccaacgatc cttatgagat gttcaggacc
     2161 gaaatattcg atgtgtaacg aagctgagga ggcataatgc aatgaaactc aaagacttgg
     2221 caaaggtcat ccgcagcaag aatgctggtc ctttctacgt gacactcgac gtaatgttct
     2281 cagacgccga gacgtatcag aaagtcaaag caactggaac actcaacgca gacctgatag
     2341 cgagccttta caatgtggcg cccgataagg tcagattctt tgaatacgac ccagcatatt
     2401 cgttcaaggc gaccattcct cggagaattg tcagcggaga tttcggcgat accgactctt
     2461 acggtgcaca acaacacgca cctctgttgg acgttgacat ttcactataa gacagagcaa
     2521 tgtatgaagc attggaggca atcagccatg tgtcagaatc agactagaat actgggcatg
     2581 gaaatcaagt actatctagt tgcgttggcc atatgcatca taggaatggc gtttgatctc
     2641 gtcggcaatg gcatgatttt cgcgataccc atgcttttgg tcctaagtca cgggctgacc
     2701 aagatcggcg acaacatacc gatctggaag gattgggtgg gtggaggggt taccctcacg
     2761 atgtttgccg gcgccatatt ggtcaagttc atccctgaga ctgctctcga gagtatcaag
     2821 aatttttatg tcgtagacga cttccttgtt ttcaccattg gcaacctgct catctgcagt
     2881 atcatgggga tggacaggaa agtgcttacg caggccgcag tcggtttcct tcccacggtg
     2941 ctagcaggag tagcttgtgc ctatctttgc ggaggattga tcggcgcgct gcttgggtat
     3001 ggattctggg atgccatact ctacatctca cagcccatca tgggaggagg catgggagcc
     3061 ggcgcaatac cgatatccaa gatctacgcc tcagccggcc tcaaggacgc agacgcatat
     3121 ctctccatgc tcgtcgccgc agtcgttcta ggaaacatca tatcgattat cgtagcggct
     3181 gtccttaaca aggtgggtga actgcgaccc gaactgacgg gcaacggcca gctggtcaag
     3241 tcggccaaat cggccacaca gagcgagtca aaagttgcag aagcccaatc cgtacatctt
     3301 acgcccgagt ccataggcat gatcatagtg tggatagggg tgttcataat tctgggcaat
     3361 cttgcgcgca agattatccc tgtgaacatc cacccctacg catggatgat catccttgca
     3421 tgcgccgcaa aaattctgaa cctgttcccg gagaagtttg ctcagactgc caggacgtat
     3481 ctgcccaagt atgtggctct ggcaatgcca cctatcatgc tgggagtagg actcttctac
     3541 actgacctcg agcagttcgc agccagcgta tcgaacccga cattcgtctt gatctgcctg
     3601 gctaccgttc ttggagcagt gcttggcgcc ggactcttcg gtcgactgtt caagttcaac
     3661 ttcgtagaat cttccatggc cgcagggctg tgcatggcta acatgggagg atctggtgac
     3721 cttgcagtcc tttcggctgg caagagaatg ggactcatgc catttgctca gatatcctcc
     3781 aggcttggtg gagccatagt catgataatc gccggagcaa tgctgtcgct gctaagataa
     3841 acacctagca accatattgg ctctggaagt attggagtga actgcgcgac tccctgtccg
     3901 ctaaggtgga tagggagttt tatttgtgtg cccggggtgg atagcggcga tgacatcagc
     3961 gctcccaccc ttgtctccca tgtggcatat tccatatggc catttgtgac aaggtcaagt
     4021 gagtgctaaa gaagtcggca catttccgtt gaggaccaaa ggaaaatcga ggatcgagta
     4081 acgaggggct acttcctgcc ctgggcgggc cgggtggcgg ggactaggga aggggagtct
     4141 tcgggattcc ggcagggtgt ctagatttta gccactttgg aaacaaaagg gctcaacttt
     4201 taggcgcggc ctgtctaaaa attcatatga aggaggccgt caagccccaa tttcaatttc
     4261 ggcagcggtg ctaccgaaga cccggtttac gtcgacggtt ggcagactca tatccgcggg
     4321 ttggttaccg caactaatac caccgtctgg agctccatca tactagactc gcacgtaatg
     4381 cctctatggg cattgtgcgt aattttttcg tggattctcc ctaccggctt ccggttcttc
     4441 ggccaccttt caccgaaacc ttggcatcaa tagaaaacca agcatctgtt cccttggggg
     4501 gtatccgtag ggaactacgt tgccctgagg caatacaggt tttattaaat tgccttggtg
     4561 gtcaagatta ccgttta
//
LOCUS       DAOSAI010000195         4569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_11943_length_4569_cov_2.870403, whole genome shotgun sequence.
ACCESSION   DAOSAI010000195 DAOSAI010000000
VERSION     DAOSAI010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11943_length_4569_cov_2.870403"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLM83_07145"
     CDS             complement(<1..871)
                     /locus_tag="PLM83_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK71215.1"
                     /translation="MTESQGEAAIQRLADVDRCRPLCLESAVERLVHNDRLRPERRRI
                     FHMTDEHRQNVEDLLSSGLKALADGNAQEARGLIDKALVLDSTRVDSLLATSLACWMA
                     GDLEAAKDAVKAASCYISDDIGELVMVGLAYARGGNDTSTAYHYLMRALSLAEGDAEL
                     TSVLADALLEIGFPDESRDAAERALSIQPELAEASLTLGSAYIALDNYEDAIEHLIRA
                     THGIPDNAVPPFLLGVALYETGQWADAARAFGEAAKREPDDHMIDVWRAKALYETGQT
                     DAAISIIDNLLEEA"
     gene            1096..1183
                     /locus_tag="PLM83_07150"
     tRNA            1096..1183
                     /locus_tag="PLM83_07150"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1130..1132,aa:Leu,seq:cag)
     gene            complement(1442..2035)
                     /locus_tag="PLM83_07155"
     CDS             complement(1442..2035)
                     /locus_tag="PLM83_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71216.1"
                     /translation="MEEEKTVNWFARLIGIITAPPVMAGAAAATVRHYDPTLFGDGTT
                     WYLVTLMLLTAIPLFSYVVAYMVPSIRAMGRKGQRKLAFIVSVICYVVGAAVCLIAKA
                     PRIVSAFFLSYLASGGLLSLINAAFKFKASGHACGFAGPVTLVSMVIGPVALWAFALL
                     PLVFWARLSMKRHSKGELWSGALVGIGATAVVMLIFP"
     regulatory      2785..2928
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3074..>4569
                     /gene="pruA"
                     /locus_tag="PLM83_07160"
     CDS             3074..>4569
                     /gene="pruA"
                     /locus_tag="PLM83_07160"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOK71217.1"
                     /translation="MDFSIVENRDRMADALATVEEEFGHYYPLIIAGQRVETEERVAS
                     VNPCAHEQVVGYVAQADRAQAEMAMEEAWKAFETWREVPAEGRAATVLRLSALMRKRK
                     AELAAWLVFEVGKNWVEADADVAEAIDFCEYYAREMIRYEGLNQVYPHPNEQNVQRYI
                     PLGVGVVISPWNFPLAILTGMTTAAVVAGNTVIIKPANTAGVIAAKLMELVDEAGFPP
                     GVINYLPSSGKTIGDYLVQHPKTRFINFTGSKDVGLHINEVAAKHQPGQIWIKRVVAE
                     MGGKDFIIVDETADLDKAAMGIVKSAFGYQGQKCSACSRAIIVDSVYDEVLEKTREMA
                     AELRVGEAAANFAVNAVIDEKAFRSISNYIDIGREEGRIVLGGDRDSSVGYYIEPTII
                     EGVSPHARVAQEEIFGPVLAFIRADSYDHAVEIANCTEYGLTGSVYSRNRARLESAKD
                     KVHVGNLYLNRHCTGALVGLHPFGGFNLSGTDSKAGGPDYLLLFMQAKA"
BASE COUNT         1017 a         1145 c         1331 g         1076 t
ORIGIN      
        1 gggcttcctc taggagattg tcgattatgc tgattgcggc gtccgtttgc ccggtttcgt
       61 agagggcctt ggctctccag acgtcgatca tgtgatcgtc gggttcacgc ttcgcagctt
      121 cgccaaaggc tctggcggca tcggcccatt ggcccgtttc gtatagagct actcctaaaa
      181 gaaatggcgg aacagcgtta tctggtattc catgggttgc tctgattagg tgctctatgg
      241 catcctcgta gttgtcgagg gcgatatagg cactgccaag cgtcagtgat gcctctgcaa
      301 gttctggctg tattgacaga gccctttcgg cagcgtcccg agattcatcg gggaacccta
      361 tttcgagaag ggcgtctgca agaactgatg tgagttctgc gtcgccctca gcaaggctca
      421 gcgcccgcat caggtaatgg taggccgtcg aagtgtcatt tcctccgcgt gcataagcca
      481 gaccgaccat aacgagttct ccgatgtcat ccgatatgta gcacgaggct gccttgaccg
      541 catctttggc cgcctcgagg tctccagcca tccagcaggc caatgatgtt gcaagcagag
      601 aatcgactcg tgttgagtca aggacgagtg ctttgtcgat gagccctcgg gcctcttgag
      661 cgtttccgtc cgccagggcc ttcagccccg aggagagcag atcttcgacg ttttgtctgt
      721 gttcatctgt catatggaat attcgccgcc tttccggccg tagccgatcg ttatgtacga
      781 gtctctctac cgcggattca aggcatagtg gacggcatct atcaacgtct gccagacgct
      841 ggatcgccgc ttctccttgt gattctgtca atttagataa tctcctgcca gggaaaggat
      901 cgagcttcct agcgggcgta ttcgagctga caactacgtg ggcagctgtg cgggttgggc
      961 agtatagagc atgcaccgtc aatggcgcgg taactattgc atactggcac gtggcgtgat
     1021 atactagctg agcaggttgg gatgtgcctt tttcacaggg tgcatccaaa gcgacaagtg
     1081 aacatgtttg atcgtgccga agtggtggaa ttggcagacg cgctagattc agggtctagt
     1141 gggggttaac cccgtgcggg ttcaagtccc gccttcggca ccattatttt gggcaggatt
     1201 gtttcctgcc cattcatttt gtttcacatc tgagggccca tgctctgagc ctggcgcaag
     1261 aaatcggttc cgatcgctgg cgccaggcat cgtgatttcc gctgtcgcag cactgaagtg
     1321 ctgagacagc ttcagatggc ctgcggtatc tggcaggcca aattggcaag cttcggcagc
     1381 gcagactgac agaccacgcg aaatcggccg ctacccgctg acaagctggc cacgccaccg
     1441 cctacgggaa gatgagcatg actacggcag ttgccccaat ccccactaat gcgcctgacc
     1501 aaagctcgcc tttggaatgc cgcttcatag aaagcctggc ccagaatacg agcggcagaa
     1561 gggcaaaggc ccaaagggca acagggccta tgaccattga cacgagagtc acagggccgg
     1621 cgaagccgca tgcatggccg cttgctttaa acttgaaggc ggcgtttatc aggcttaata
     1681 agcctcctga tgcaagatat gatagaaaga atgcagacac aatccgcgga gccttggcta
     1741 tcaggcaaac tgcggcgccg actacgtagc atatgacgga aactataaat gccaattttc
     1801 tctgaccttt tctgcccatc gctctgatgc tcggcaccat gtacgctact acatatgaaa
     1861 acaagggaat cgcggtgagg agcatcaatg tcaccagata ccacgtcgtg ccatccccaa
     1921 acagcgtagg atcgtagtga cgaaccgttg ctgcagcggc gcccgccatt acggggggag
     1981 cggtaatgat gcctatcaat cgggcaaacc agttcactgt cttttcttct tccataagct
     2041 gtcctccgtt cttcagcgct taccgagtaa gcctctattg gatagtgcgc ggctagggag
     2101 tttgggttgc ttgaagttca ggcctgaccc gcccggtgag gatgggatgc ttctttggaa
     2161 ctgttcacgt tgcacggtac agatgcgatg gcggaacgaa tatcggtcgg aagcgagcag
     2221 cgcgagcact taactttaat aatattcttc actggacagg aggcttcctg cctcgaaagc
     2281 tggaagccca atacttgcag accatttcaa tgcggttctc gattgcgttg tcctatctgc
     2341 ctgaccggtg cgtataaccc tgcggtagct gcttgatata catagaacag ggagacaggc
     2401 ttctttgacg gttggcgcag ccccgaaggt tcttcattcg attgacgatt ccgcaggtca
     2461 ttcatcacag ccattaccga tggagtctta gccgcctaca taacccttct aggtgctttt
     2521 cggatatcgg ggcacgaaat ctggccgctc acgaatgctc tgacatatcg tgaatagcat
     2581 tatgtcaaga actcgcggcc ttgcgaggta gatgcgctga tggctggaca gtcttgatgt
     2641 agtaggttct gtgaaggctg gaaaggtcaa tatgtattcg ggcacgcgca gtgaagttgg
     2701 ctgcagggtg cgcgagttcg gcttacttgc aatatattgt aagttgagtg caagttgtag
     2761 gattcctgcc atttttgtcg aatctgccat atagcgcctc cgagtcatct cttcttaccc
     2821 gatgatgctg gagcggacat gaccggtggc aactacgttg ttgcctgagg gtctggctgg
     2881 aaacggcctg gcctcccgtg tttggaaagg agcgcaggca ggctccgtca catgcgcgtt
     2941 cggtggcgga cagggcatgc catgcgcttg caagacacga aacgtctctt tgttcactgt
     3001 gttgtgatgc tgaccctcgg caacgttagg aaaggggtct attccgttga agcagttttc
     3061 caatgaacca ttgatggact tctcgattgt ggaaaatcga gacaggatgg ctgatgctct
     3121 ggcgactgtg gaagaggaat tcggacacta ttatcccctt ataatcgcag gtcaacgcgt
     3181 ggaaaccgaa gaaagagtcg cgtcggtcaa tccttgcgca cacgagcagg tagtgggcta
     3241 tgtcgcccaa gctgaccggg cccaggccga aatggctatg gaggaggctt ggaaggcttt
     3301 cgagacctgg cgtgaggttc ccgctgaagg acgcgctgct acagtcctga ggctgtctgc
     3361 gctgatgcgc aagcgcaaag cggaactcgc tgcctggctc gttttcgaag tgggcaagaa
     3421 ttgggtggag gcggatgcag atgtagctga ggccatcgat ttctgcgagt actacgctcg
     3481 ggagatgata cggtatgaag gcctcaatca agtgtatcca caccccaacg agcagaatgt
     3541 gcagcgctat atccccctcg gagtaggggt ggtgatcagc ccgtggaatt tcccgttggc
     3601 gatcctcact gggatgacga ctgccgcggt agtggccgga aacactgtga tcataaagcc
     3661 cgcaaatact gctggtgtga tagcggccaa gttgatggag ctcgtggatg aggcaggttt
     3721 tcctccaggc gtcatcaact acctgcccag cagcggcaaa accataggtg actacctagt
     3781 ccagcatcct aagacaaggt ttatcaactt taccggatcg aaagatgtgg gccttcacat
     3841 taacgaagtg gccgccaaac atcagccagg tcagatatgg atcaagcgcg tagtggcaga
     3901 gatgggcggt aaggacttca tcatcgtgga tgagacggct gatttggaca aggcggctat
     3961 ggggatagtc aagtcggcct tcggatacca agggcaaaag tgttctgctt gttcaagagc
     4021 gataatcgtg gactcggttt acgacgaagt tctcgagaag acgagagaga tggctgccga
     4081 attgagagtg ggggaagctg cagcaaactt cgccgttaat gcggttatcg atgagaaagc
     4141 tttcaggagc atttccaact atatcgatat tggccgtgaa gaaggaagaa ttgtgctggg
     4201 cggcgatcgt gattccagtg tcgggtatta catagagccc accatcatag agggtgtgtc
     4261 tccccatgca cgcgtggccc aggaggagat tttcgggcct gtgctcgcat ttatccgggc
     4321 ggacagctat gaccatgcgg tggagatcgc caattgcacg gaatatgggc tcacgggttc
     4381 cgtctacagc aggaataggg cgcgattgga gtctgccaag gacaaggttc atgtgggaaa
     4441 tctatatctt aacaggcatt gcaccggggc attggttggg ctccatccgt ttggcggttt
     4501 caacctgagc gggaccgaca gcaaggcggg gggccccgac tacttgctcc ttttcatgca
     4561 ggctaaagc
//
LOCUS       DAOSAI010000196         4507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12134_length_4507_cov_3.628931, whole genome shotgun sequence.
ACCESSION   DAOSAI010000196 DAOSAI010000000
VERSION     DAOSAI010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12134_length_4507_cov_3.628931"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLM83_07165"
     CDS             complement(<1..312)
                     /locus_tag="PLM83_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOK71218.1"
                     /translation="MSKDCVKLQFDDCVATITISRPTALNALNNTVLAELASAIEEVG
                     ENRACRALIVTGEGSKAFVAGADIQEMASLTPDEAVAFSRHGQRVFSAIERLPIPTIA
                     AV"
     gene            complement(342..1193)
                     /locus_tag="PLM83_07170"
     CDS             complement(342..1193)
                     /locus_tag="PLM83_07170"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOK71219.1"
                     /translation="MSIDRILVVGAGQMGSGIAQVAAEAGLEVSLADLSVDYARKGVD
                     RILKSLNRQVEKGRLTEEDKEDTVSRINCVPDYSAGGDVDLVIEAIVEDPKVKSKLFA
                     ELASICPPEAIFASNTSSVSITSLARDSGRPDRFIGMHFMNPVPVMQLVEVVRGLATS
                     DETCEIIVALARRMGKEPVVVADSPAFIVNRMAIPMINEAIFALSEGISDAEGIDACA
                     KLGLNHPMGPLALGDLIGLDTVLSILEVMFQETGDPKYRPCPLLRRMVAAGRLGRKSG
                     RGFYEYS"
     gene            1382..>2713
                     /locus_tag="PLM83_07175"
     CDS             1382..>2713
                     /locus_tag="PLM83_07175"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOK71220.1"
                     /translation="MKRYLFTGGKVYTASARQPFCQALACVGKRIIAVGSESNVLSMT
                     DDTFERIDLRGKVLIPAFIDSHIHFKFYSMCQTSANLEGARDPHDAALRISKAMEGIK
                     PGAWVLGWGWNKNEWTQGRLPDRSYLDSICPRNPVALFSKDLHALWVNTMALRLAGIG
                     LDTPNPEGGEIVRDEDSRQATGVLKESAVELVTRVIPEPTFDAVVLAMQKGQSELHKL
                     GIAGICNMEDALAFEALQAMRSSGRLKLRVTQAIPKQNLDDAIDLGLGTGFGDEWLRV
                     GPVKLFADGALGSQTAHLLRPYEGNKENVGIAVTPKEELAELVGRAVLAGLSCAVHAI
                     GDRANRDVLDIFESVKEESAARHLHHRIEHAQLLHPEDVRRFANLGVVASMQPVHILT
                     DIPVAEKYWGRRSRWAYVFRSLTKAGTPLAFGSDAPVETPDPIRGIYAAVAR"
     assembly_gap    2714..2723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2724..2978
                     /locus_tag="PLM83_07180"
     CDS             <2724..2978
                     /locus_tag="PLM83_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK71221.1"
                     /translation="LDGTPDSGWYPEERLTVEEAIRAYSVGSAMASGERMGAGVLVPG
                     SYADIAILSKDIFSIPSSDILDVRVVGTMVGGEFVYRDSI"
     gene            3234..3854
                     /locus_tag="PLM83_07185"
     CDS             3234..3854
                     /locus_tag="PLM83_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71222.1"
                     /translation="MEPIPVSLAPGEYEVVKTEYRIHAGMYVSFRRVIGKAVTDSKWQ
                     VTVQSSEYPKWVEIGWVWPRSDGTAPTPTVRKLTGLERGRTFGPLYGGNDPEETDWTA
                     PWLSREVLRELRTGRVAYNFKMGTFSLTGVLADDLRVEEEVLYPIDLNGRRVYLPAYK
                     CAKGQIVVWNNIENPLVLEYKPFGIPLITGVFGWKAEAISVRGGQL"
     gene            4013..4285
                     /locus_tag="PLM83_07190"
     CDS             4013..4285
                     /locus_tag="PLM83_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK71223.1"
                     /translation="MEIIADYGLGKTFAAKQKEPGIAGSVKAVLSPRFKDVEAVRNVS
                     FSIDEGEAIAFIGANGAGKSTKVLLHPTTGEAVVLGIVPWRDRQQI"
BASE COUNT         1008 a         1073 c         1341 g         1075 t
ORIGIN      
        1 cactgcggct atggttggaa tcggcaaccg ttcgatagcg ctgaataccc tctggccatg
       61 ccgggagaag gcgacggcct cgtcaggagt aaggcttgcc atctcttgga tatctgcacc
      121 agctacaaac gctttgctac cttctccggt gacgatcagg gcacggcacg cacgattctc
      181 tccaacctcc tcgattgccg aagcaagttc agccagaact gtattgttta gggcgttgag
      241 cgccgttggg cgggatatgg tgatcgttgc tacacaatcg tcgaactgaa gtttgacaca
      301 gtctttgctc aaggtttgcc acctcctagc gaagatttga atcaggagta ttcatagaat
      361 cctcggcctg atttgcgtcc aagtctgcca gcggccacca tcctccgcag gagcgggcag
      421 ggcctgtact tgggatcccc ggtctcttga aacatgactt cgagtatgga cagaaccgta
      481 tcgagaccga tcagatcccc gagggccagc gggcccatgg gatgattgag ccccagcttt
      541 gcgcaggcgt ctatgccctc ggcatctgat atgccctccg aaagtgcgaa tatggcttca
      601 tttatcatcg gaatggccat cctgttgact atgaaggcag gcgaatccgc gacaacaaca
      661 ggttctttgc ccattctcct ggcgagggcg acgatgattt cacatgtttc atctgaggtc
      721 gcgaggccgc gtacaacctc cacgagctgc atcacaggga cgggattcat gaagtgcatt
      781 ccgatgaaac gatcggggcg ccccgaatca cgggccaggg acgttatgga tactgaggac
      841 gtgttggagg cgaatattgc ttctggaggg catatcgacg ccagttccgc gaagagcttg
      901 gatttgacct ttggatcttc aacgattgct tcgatcacca ggtctacatc gccgcctgct
      961 gagtagtccg gtacgcaatt gatcctcgat actgtgtcct ctttgtcttc ctcagtcagg
     1021 cgtccttttt cgacctgtcg gttgaggctt ttgagtatgc ggtctacgcc tttgcgagca
     1081 tagtctactg atagatcggc tagggacacc tcgaggccgg cctcggctgc gacctgcgcg
     1141 atcccggagc ccatttgacc tgcgcccacc acgagaattc tgtcgatgga caagaaacaa
     1201 tcaccctttc atccgcgaca ctctgttgca agccaattgc agaggtcgga gatagttgat
     1261 ccacactaac gattattact tctatagccc ctcttttgac tccttctctg ctcctctggc
     1321 aggagaagag gaaaggttga tgaaatagtc aatcacccga ggaatgctgg gagggagctt
     1381 cttgaagagg tacttgttca cgggaggcaa agtatacact gcatctgcac gtcaaccatt
     1441 ttgtcaggct ctggcttgtg taggaaagcg gataatcgct gttggttctg aatcgaatgt
     1501 gctgtcgatg acagatgaca ctttcgagag gattgacctt cgaggcaagg tcctgattcc
     1561 tgctttcatt gattctcata tacatttcaa gttctactcg atgtgccaga cctcagcaaa
     1621 tcttgagggg gcacgcgatc cccatgatgc cgctcttcgc atcagcaagg ccatggaagg
     1681 cattaagccc ggtgcgtggg tgctaggatg gggatggaac aagaatgagt ggacgcaagg
     1741 gagactgccg gaccgctcat atctcgactc catttgccct agaaacccag tggccctttt
     1801 cagtaaggat ctacatgctc tctgggtgaa taccatggca ttgcgtcttg caggcatagg
     1861 tcttgacact cctaatcctg agggcggcga gatagtcagg gatgaagatt cgaggcaagc
     1921 tacgggggtt ctcaaggaat cagctgtgga gctggtgact agagtaattc cagagccaac
     1981 attcgatgca gttgtcttgg ctatgcaaaa aggacagtcg gaacttcata agctgggaat
     2041 tgcagggatc tgcaacatgg aggatgctct ggcattcgag gctttgcagg cgatgaggag
     2101 ttcggggcgg ttgaagctcc gcgttacgca ggctatcccc aagcaaaact tggacgatgc
     2161 gatcgacttg gggcttggaa ctggttttgg ggatgagtgg ctgagggtgg ggccggtgaa
     2221 actctttgct gacggagcat tgggttctca aactgcacac ctgctcaggc cttacgaagg
     2281 caacaaggag aatgtaggca ttgctgtgac tccaaaggaa gaactggctg agctggtcgg
     2341 tcgtgctgtt cttgcaggct tgtcctgtgc agtccatgcc atcggcgacc gcgctaacag
     2401 ggatgtgctc gatattttcg agagcgtgaa agaggagagc gctgcgcgcc atctgcacca
     2461 tagaatagag catgcacagc ttctgcatcc cgaagacgtt cgcaggtttg ccaatctcgg
     2521 ggtagtagcc tcgatgcagc cggttcatat actcactgac attcccgtag ccgagaagta
     2581 ttggggcagg aggagtaggt gggcttatgt cttccgcagc ctgacaaagg ccggaacgcc
     2641 acttgccttc ggctccgacg ccccggttga gactcctgac cccatccgcg gcatatacgc
     2701 cgccgtcgcc agannnnnnn nnnctggacg ggacgccaga ttcaggatgg tatcctgagg
     2761 agaggctaac agtggaagaa gccattaggg cctactctgt tggctcggcg atggcttcgg
     2821 gcgagcgaat gggagctggg gttctggttc cgggatcata tgccgacatt gctatattgt
     2881 caaaagacat attttcaatt ccttcatccg atatccttga tgtgcgcgtg gtcggcacca
     2941 tggtgggagg ggaatttgtt tacagagact ccatctaggc aagcatctgg gcgctgccat
     3001 acgaggcatt ggcgcggctg agcggatagc gaggaatggg catggcccgg aggcgaagtg
     3061 gagagtgtgt caatattggg accggtctgg acgtacgttg tttgggccag gccgcaacta
     3121 tgaccgttca aggggagatt ggtttgaaga aaagggtttt gctggctttg atatgcttga
     3181 cttcagtgtg cgtgctatgc gttgggccat cggaagctca aacagtacgg ccgatggaac
     3241 caattccagt cagtttggct cctggagaat atgaagtagt caagacagaa tacaggatcc
     3301 atgcgggtat gtacgtgtcg ttccgacggg tgatcggaaa ggctgttact gattcgaagt
     3361 ggcaggtgac tgtccaatcg tccgaatacc ccaagtgggt ggagatagga tgggtatggc
     3421 ctagatcgga cggcaccgcg cctaccccaa ctgtgaggaa gttgacaggg cttgaacgtg
     3481 gccgcacatt cggaccattg tacggaggca atgatcccga ggaaacggat tggactgcgc
     3541 cgtggctatc acgggaagtc ttgcgtgaac ttcgcacggg aagagttgcc tacaacttca
     3601 aaatggggac ttttagcctg accggagttt tggcggatga tctccgtgtt gaggaagagg
     3661 tgttgtatcc catcgatctc aacggcagac gggtctatct tccggcgtat aaatgcgcca
     3721 aggggcagat cgtggtctgg aataacatcg agaatcccct ggtattggag tacaagccct
     3781 ttgggattcc tctgataacc ggagtgtttg gctggaaggc agaggccatc tctgtccggg
     3841 gtgggcagct gtagcaagta gccaattcgg gagcacatgg atttggccaa ctgctaatca
     3901 attgctagac agacgtgttc tgggcgagag cttttccggc ccctgttggt gtcaggttgc
     3961 ctctgcatac agcatctggg cttttcaagg aacgtttgaa ggcgcgaggt atgtggagat
     4021 aatcgcagat tacggattag gtaagacatt tgctgcgaag cagaaagagc cgggaattgc
     4081 gggaagtgtg aaggccgttt tatccccgcg tttcaaggac gttgaagcgg ttaggaacgt
     4141 gtcgttctca atcgatgagg gagaggcaat tgcatttata ggggccaatg gcgcaggaaa
     4201 gtcaacaaag gtgttgcttc atcccacaac tggagaggcg gtcgttctgg ggatagtccc
     4261 gtggcgcgat aggcaacaga tttaaagaag gatcggcaca gtctttagac agaggtatca
     4321 gctttagtat catctcctgc cccgcgatag ctttgatctt ctgctggtat ccacgatatg
     4381 tcgcaaggcg tttttcgtgc gcgtatgggg atgcttgcgg acatattcga gctgggcgaa
     4441 gtgatgtcga ctccgcttct ttctgagaga ctgtgttgct cggggtacac ggttgtgacc
     4501 gaagaga
//
LOCUS       DAOSAI010000197         4478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12243_length_4478_cov_4.078680, whole genome shotgun sequence.
ACCESSION   DAOSAI010000197 DAOSAI010000000
VERSION     DAOSAI010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12243_length_4478_cov_4.078680"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(341..580)
                     /locus_tag="PLM83_07195"
     CDS             complement(341..580)
                     /locus_tag="PLM83_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71224.1"
                     /translation="MRRNAGHVGWIRRRPISAMFSIGSAYVAAKAAGYSIKRGFAIGQ
                     FRCQQVVAVDKPTLLSGPAIAFGFWVGCWVLALSN"
     gene            complement(587..>4478)
                     /locus_tag="PLM83_07200"
     CDS             complement(587..>4478)
                     /locus_tag="PLM83_07200"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOK71225.1"
                     /translation="DLIVAEREPIDERSIWGRPDPQLYVVWRDILSQDDSADAVSRLA
                     EAAIEHRVPNEVVNRAYERFSSSADGFLYGVMSGDEYVVRTQQLLAEDPLALTELAGM
                     LFGSGMSPHTSEQSAVSLIGLAVRARPSEDSSPLLPARYHLMLKALGGAWLSFLPEPK
                     VHLDQVARVTWGDKTAMAYEAGVCQKCGAPYILGARVNSDSGNGWHLAPVSADSNDRE
                     ARYYAVLAPEDPSEMRPDEDEDPIAYDTSKSLYQLCPACGDIRRADSLGIECSCGVEK
                     VRLLETKSASRLIGRCIVCGGQNTKHGIVRRVSSYPEAAASVIATSLYQELSFDESGM
                     ESRNGQSAPEVQYDEDGWVIDVSAPRSEDAGPQKLLVFSDSRQDAAYFAPYMQTSYDR
                     MLRRNILLGAAREAIDELPDLNIMVPDLWTHAELKLRPVLPTGYDALVEAQKWVLYEF
                     TGRDKHGLERLGLLGFELERPERWTVPLHLQRKWGLSESESWDLYQALLDTFRTSGAV
                     SVPRGVDPEDELFEPGNLLGSLTKADRDRRYVKAWLPTSSSTANKRSDYLTRLAQQIG
                     LWDDPEKAACDLLSDIWDRDLMPHNARSLWNDYFEPVVDKGGGVSYRLVPNKWRILAY
                     GSRNEPKWYVCDKCGRLALRNIRGVCPQYRCMGRLNQADMSERLESNHYVRLYESQNF
                     PKEMVSREHTAQLRPGAAAKSQTEFQEGRINVLSCSTTFELGVDLGDLEAVLMKNVPP
                     SPSNYIQRAGRAGRRAGTAAMVVTYAQDRPHDAFHHRYPERMIKGHIKPPHFDINNEK
                     IVLRHMYAVALADFWYKNPNYLDKVKDFFFPDDADSAVDSLIQYLDSRPESIRVSLER
                     VVPEKLIEPLGVDTWQWVDSLVGDGGPLAVARMEVENDIKRLDARYRELAAQRKKSDY
                     VLRLIETMQKRYLLNYLSSKNVLPRYGFPVDVVTLETKNNHYATDDICLQRELKIAIS
                     EYAPDGEVVAGGEVWTSRYLERMPGKELPSYWYASCPGCGNHVTKLDVRDGDARAICS
                     VCGREVECRESFIVPEFGFLVDPNKPAVAGGRRPQRTHSTRVFFSGIEDDYTSGDQGF
                     NFPNGRVIISTSKRGKLTVINDARGAKFRVCQSCGYACIAEGKDPVKHHKNAYGKECS
                     TKSLTRIALGHEFLGDIARIRFEPCTRVDQGFWYSLLYAMIEGACEALDIDRTEIDGC
                     VQWGDSAGQPTYLILFDSVSGGVGHVQQLGREEALRDTLYTAYIKMASCTCGDDAGNG
                     SCYSCLRNYTNQFCHDVLNRGLVVRFLKELGVEG"
BASE COUNT         1026 a         1288 c         1038 g         1126 t
ORIGIN      
        1 tcctggcatt taccggcgct ccagatttca gaagcacaac aacgctgcct gtatggccgg
       61 cacgagcagc gagatgtagg ggggtattac cgtgtggatc acgtattcga aggttagctc
      121 ctcttttcac aagagacaca atatcgggta tgtttcctct ggaagcagcg tagtgcaatg
      181 gcgcatagcc attctcatcc gtgcggttca cgggattcag cggtgagcgg acgcacaaaa
      241 ctatagccgc tattgcggat agagcacaca ataacgcttg aaccattcgc ctggcattca
      301 caataaatca gtcacctcga gaacgatttt tccatactct ctagttcgac aatgcaagca
      361 cccaacaacc tacccaaaac ccaaacgcaa ttgcagggcc ggacagtaat gtcggcttat
      421 cgacagcgac cacctgctgg catctgaact gcccgatagc gaatcccctc ttaatggaat
      481 atcccgccgc ttttgctgca acatatgctg acccgatgct gaacatggcg cttatcggtc
      541 tccttcgtat ccatcctaca tggccagcgt ttctccgcaa tgtcagtcag ccttccacac
      601 ctagttcctt caggaatcgc accaccagtc ccctgttaag tacgtcgtgg cagaattggt
      661 tggtgtagtt tctcaggcaa ctgtagcaac tcccattgcc cgcatcatcc ccgcaggtac
      721 aagaggccat cttaatatag gctgtgtaca gggtgtcacg gagtgcctcc tcacgtccta
      781 actgctgtac atggccgact cccccggata cagagtcgaa caatatgagg taagtaggct
      841 ggccggcaga gtcgccccac tgcacacagc catctatctc cgttcgatca atgtccagcg
      901 cctcacaagc cccttcaatc attgcgtaga gcagcgaata ccaaaagccc tgatctactc
      961 gcgtacacgg ctcaaatcga attctcgcta tgtctccgag aaactcatgt cccagcgcta
     1021 tccttgtcaa ggactttgta ctgcattcct tcccataggc gttcttatga tgtttcacgg
     1081 ggtctttgcc ttctgcaata catgcatagc cacatgattg gcacacgcgg aatttcgcgc
     1141 ccctcgcatc attgataact gtcagcttcc ctctctttga cgtgctgata atgacgcggc
     1201 catttgggaa attgaatcct tgatcgccac ttgtataatc atcctcaatc cccgagaaga
     1261 aaacccttgt tgagtgtgtc cgctggggac gtcggccgcc tgcaaccgca ggcttgttcg
     1321 ggtctactag aaagccaaac tccgggacta taaaggactc cctgcattcg acttctctgc
     1381 cacagacact acatatagcc cttgcatcgc catccctaac gtccagtttg gttacgtggt
     1441 taccacaccc cggacaacta gcgtaccaat agctaggcaa ctccttcccc ggcatgcgtt
     1501 ccagataccg gctggtccac acttctccgc cagctaccac ctctccatcg ggagcgtatt
     1561 cggaaatggc aatcttcagt tcgcgctgta gacagatgtc gtctgtagca tagtgattgt
     1621 tcttcgtctc caaagtgacc acatcgacag gaaagccata tctgggcagg acattcttgc
     1681 tagataggta gtttaagaga taccttttct gcatcgtttc tattagcctg agcacgtagt
     1741 cagatttttt ccgctgagca gccagttctc ggtacctggc gtcgagcctc tttatgtcat
     1801 tttccacttc catgcgcgca acggcgaggg gacctccatc gccgactaaa ctatcgaccc
     1861 attgccaggt atctacgcct agcggctcga ttagtttctc gggcactacc ctttcaaggc
     1921 tcactctgat agattccggt ctcgagtcta gatactgaat caaagagtct actgccgaat
     1981 ccgcatcatc tgggaaaaag aaatccttca ctttatccag gtagtttggg ttcttgtacc
     2041 agaagtccgc caaggccact gcatacatat gccgcaggac tatcttctca ttgtttatgt
     2101 caaaatgggg aggcttgata tgccccttga tcatcctctc tggatatctg tggtggaacg
     2161 cgtcgtgagg cctatcctgc gcatatgtga ctaccatggc ggcagtgcca gcccgcctgc
     2221 ctgctcgacc ggccctttgg atgtagttgg acggcgaagg cggaacgttc ttcatcaaca
     2281 cggcttcgag atcaccaagg tcgacgccga gttcaaatgt tgttgagcag ctcaatacgt
     2341 tgatgcgccc ttcttgaaac tctgtttggg attttgccgc cgctcccggt ctgagctggg
     2401 ctgtatgttc gcgggatacc atctctttag ggaaattctg actttcataa agacgcacgt
     2461 agtgattgga ttcgagtctt tcggacatgt cggcctggtt gagtctaccc atgcacctat
     2521 actgagggca tacgcccctg atattccgca atgccagcct accgcatttg tcgcacacat
     2581 accacttcgg ttcatttctg cttccgtaag ccaatatccg ccacttgttt ggaactagcc
     2641 gatatgaaac gcctccaccc ttgtctacca ctggttcaaa gtagtcgttc cacaggcttc
     2701 ttgcattatg tggcatcaag tctctgtccc atatgtcgct gagcagatcg caagcagctt
     2761 tctccggatc atcccaaagc ccaatttgct gagcaaggcg tgtcaagtaa tcggaccgct
     2821 tgttcgcggt cgagctgctc gtgggaagcc aagcctttac atagcgacgg tcgcggtcgg
     2881 ccttggttag tgatccgaga agatttcccg gttcaaacag ttcatcctct ggatccacgc
     2941 cgcgtggcac gcttacggct ccacttgtcc ggaacgtgtc tagcaatgcc tggtacagat
     3001 cccatgattc ggactccgac agaccccatt tcctctgaag atgtaggggc actgtccatc
     3061 gctcaggccg ttctaactcg aacccaagca gtccgagacg ttcaagacca tgtttgtcgc
     3121 gtccagtaaa ctcgtacagc acccatttct gagcctctac tagcgcatca taacccgtcg
     3181 gcaacactgg tctcagtttc agctccgcat gtgtccatag atccggaacc atgatgttca
     3241 ggtcaggcaa ctcatctatt gcctctcggg ctgctcctag aagaatgttg cgccgcagca
     3301 tccgatcata gcttgtctgc atgtatgggg cgaagtaggc cgcatcctgt cgactgtcgg
     3361 agaagacaag cagtttctgt ggtccagcgt cttcactcct tggggctgag acgtctatca
     3421 cccagccgtc ttcatcatat tgcacttctg gggcactttg accgttacga gattccatac
     3481 ccgattcatc aaagctaagt tcctgatata gggatgtagc gataacgctt gccgcagctt
     3541 caggataaga tgacactctg cgtacaattc catgcttcgt gttctgtcct ccacacacaa
     3601 tgcagcggcc tatcaagcgt gaagcagact tcgtctctag aagtcggact ttttcgacgc
     3661 cacagctaca ctcaattccg agtgaatctg cacgtcgaat atctccacaa gcaggacaca
     3721 gctggtacag ggatttgctg gtgtcgtacg cgataggatc ttcatcttcg tcgggcctca
     3781 tttcagaagg atcttcagga gccaacactg cgtagtatcg tgcttcccta tcattgctat
     3841 cagctgaaac tggcgccaaa tgccagccat tgcccgaatc agaattcact ctcgcaccaa
     3901 gtatataggg cgctccacat ttctggcaca ctccagcctc atatgccatg gcagttttat
     3961 cgccccatgt gactctagcc acctggtcaa gatggacttt gggctcaggc aaaaaggaca
     4021 accaggctcc accaagtgct ttgagcatca gatggtagcg ggctgggaga agcgggctgc
     4081 tgtcctcgga aggcctagcc cttacggcca aacctattaa tgaaactgca ctttgctcgg
     4141 aagtatgagg cgacatacct gaaccgaaga gcatgccggc aagctcagtg agggcgagcg
     4201 ggtcctcagc aagaagctgc tgcgtgcgaa cgacatattc atcgcctgac attactccat
     4261 agagaaaacc gtccgctgat gaactaaacc tctcataagc tctgtttact acttcattgg
     4321 gcacgcgatg ttcaatcgcc gcctccgcca gtcttgatac ggcatctgca ctgtcgtctt
     4381 gcgataagat gtcacgccag acaacataaa gctggggatc cggtctgccc cagatacttc
     4441 tttcgtcaat gggctctctt tcagcaacga tcaaatct
//
LOCUS       DAOSAI010000198         4470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12271_length_4470_cov_2.624236, whole genome shotgun sequence.
ACCESSION   DAOSAI010000198 DAOSAI010000000
VERSION     DAOSAI010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12271_length_4470_cov_2.624236"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2605
                     /locus_tag="PLM83_07205"
     CDS             <1..2605
                     /locus_tag="PLM83_07205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOK71226.1"
                     /translation="LAANHNRTVRMTVSMQLDACRVAGIDLDWLDRIVFDWRMHGIVR
                     SQHNLFVLSISREQQAKDLSSRVSRNLERRRAVLDHCEQEWAGGSSTVEADLMPRQQV
                     RMALGSLARFGLVEWEDPSEQREAYRVRFVAPERYDACRIANLKLGLRQTANDARMNC
                     MEQLFSESREPESVWTFLEDYFSKPSPGPYEEADARMILRGLTEQQQRAVTADIREPL
                     LINAAAGTGKTHVLARRMLLMQAVDKVDGDKILVLSFSRAGARAIGERAAELARDMKL
                     RPVRAMTFHSFCYHILRMRGLDLSLVGVKRIPKKLQTHNVYLDNLWLNQVLVDEYDSI
                     LGQLPPSGSRAESVPEKIRDYAELFDSIRSGHPDLDFVVIKPDDLDRPGVSKELRLRD
                     GEGSIPTEAVKRAFTLYLAQLQRRQLIDYAGMVSAALEGLRSDIALRTRVQAGISHLL
                     VDEYQDTSRAQEEVMRLVIGPATGLTVVGDSDQTIYSFNGSDVRNILQFEERNRLIWS
                     HKKTNIVPLEENFRSAPRILAVASRVIERNKDRLPKRLVPAAVKLASERQAYRERNAL
                     VRVAAVSPVCGAESALACAARTVEQWISEGVAPEEIAVLSRINPESNPPKALLNQLRE
                     YFATAGVPVADVPSVIRKNDPILGRLRKLANSDPQLKVADLLDGMPATAERFFNGIDP
                     KEVQAFLYDGVERGVCTVAELYEEIEAHLSDRADNGGSSEAGVTLKTIHQAKGEEYRR
                     VVVMYLAQGHFPPAQATDMEEERRLLYVALTRAEEEVTVIGEPGSLLMTELKESLGTD
                     GLVFNWSGRLDSPTQGEAGLYEECFEEDVAYSNTEPMEILQDSASDRSDAMTVAETGL
                     MEMFEDFIKPD"
     gene            2642..4066
                     /locus_tag="PLM83_07210"
     CDS             2642..4066
                     /locus_tag="PLM83_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK71227.1"
                     /translation="MEAVELTEDQRQLVEYRVDKNLLIRGEPGSGKTTVLAARAAHMD
                     SAMFSGSVLVIAYNRALAGYIRRLLGEFGGSAKIDVDTFHGWAMTFADCLGYGPARIV
                     QRTERDAKLEEILRQMGPKWGQNHLLGEPTQFWSQEVEWIMGQGISSLDKYMNAVRTG
                     RGTAVQVRGEDRQLVWDVFTAYRDWLAHDNACDIHDFGGMVRRAVLKADRKYPADLAY
                     DHILIDEVQDFDLSWLLALAPMARVSVTLAGDLGQRIYRRSFTWRAAGIDISAASSRQ
                     LRGSHRTTREIMQVAMVISTNTDLKGDEDFVAPSLPDRVGPRVSRIKRSGDRIAEKAA
                     VSRVTELARRYRGDSICLAVPFNARLRRLTDELISAGVRAVNASGDALAHLAEGTVAV
                     TTYHQLKGLEFDHVILTGLDDHTMPGFYFRIEDSESESEKEQFLRRLVYMAMTRARKS
                     VTLVGSTPFCRFFNDVPPDYIEEI"
BASE COUNT         1075 a         1116 c         1331 g          948 t
ORIGIN      
        1 gctagcggca aaccacaacc ggactgtccg aatgacagta tcgatgcagc ttgatgcgtg
       61 cagggtggcg ggcatcgatt tggactggtt agatcgaatc gtattcgatt ggcgaatgca
      121 cggaatagtc cgttcccaac ataacctttt tgtgctttct atcagccgtg agcagcaggc
      181 aaaagacttg tcttcccgag tgtcacgcaa tctggagagg aggagggcag tactagacca
      241 ttgcgagcag gagtgggctg ggggttcatc gacagttgaa gcagacctga tgccacgtca
      301 gcaggtgaga atggccttgg gctctctggc cagattcggg ctagtggaat gggaggaccc
      361 gagcgaacag cgtgaagcct acagagtgcg attcgtggct cccgagagat atgatgcgtg
      421 cagaattgcc aacctaaaac tcgggctgag acagaccgcc aacgatgccc gtatgaactg
      481 catggagcag ttgttttcag agtcccgcga gccggagagt gtatggacat tcctggagga
      541 ctacttctcc aaacccagcc ccggcccgta cgaagaggcg gatgcccgca tgatcctgcg
      601 cggactcact gagcagcagc agcgagcggt aacagccgac atccgcgaac ccctgttgat
      661 caacgctgcg gccggcaccg gcaaaacaca tgtattggcc aggcggatgc tgctgatgca
      721 ggctgtggac aaggtagatg gtgacaaaat cctagtgctc agttttagcc gcgccggcgc
      781 tcgggccata ggtgaacgag cagctgaatt agcgagggac atgaagctga gacctgttcg
      841 agccatgaca ttccattcgt tttgctatca tatcctccgc atgcgaggac ttgacttatc
      901 acttgttggg gtgaaacgaa tccccaaaaa gttgcagaca cacaatgtgt acctggacaa
      961 cctgtggctc aaccaagtcc ttgtcgacga atatgattcg attttggggc agttgccgcc
     1021 ttcgggcagc cgagcggaga gcgtgcctga gaagatacgg gattatgccg agcttttcga
     1081 ttcgataaga tcgggccatc cagacttgga cttcgtggta atcaaaccag acgacctgga
     1141 tcggcccggt gtgtctaagg agcttaggct ccgagacggg gaaggttcga tccccactga
     1201 ggcggtgaag agagccttca ctctgtacct cgcacagctt cagcggcgcc aactaattga
     1261 ctatgctggc atggtcagcg ccgcgctgga gggtttacgg tctgatattg ccctgagaac
     1321 gcgggtgcaa gccgggatat cccatctttt ggttgacgag tatcaggaca catcgagggc
     1381 tcaagaagaa gtaatgcgcc tggtgatagg cccagcaacc gggcttacgg ttgtgggaga
     1441 cagcgatcag acaatatatt cgttcaacgg ttccgatgtt cgcaatatcc tgcaattcga
     1501 ggagcgcaac agactcatat ggagccacaa gaaaaccaac atcgtgccgc ttgaggagaa
     1561 cttcaggtca gccccgcgaa tccttgcggt ggcttcaaga gtaatcgaga ggaataagga
     1621 tcgtttgccc aaacggctgg tgccggccgc tgtaaagctt gctagcgaga ggcaggccta
     1681 ccgtgaacgt aacgcattgg tgagagttgc cgcagtttcc ccggtctgcg gggcggagtc
     1741 cgccttagcg tgcgctgcgc gcaccgtgga gcagtggata tccgaagggg tagcccccga
     1801 ggagatagcg gtcttgtcca ggataaaccc ggaatccaat ccccccaaag ctctcctcaa
     1861 ccaattgcgt gagtattttg ctacagctgg cgtaccggtg gccgatgtgc ccagcgtcat
     1921 aagaaagaac gacccgatcc tgggcaggct caggaaactg gccaacagtg atccccagct
     1981 taaggtcgcc gacctcctcg atggaatgcc ggctactgcc gaaagattct tcaacggcat
     2041 cgaccccaag gaggtacagg cattcctgta tgacggagtg gaacgcgggg tttgcacagt
     2101 tgccgaactc tatgaagaga tcgaagcgca cctcagcgac agagcggata atggggggtc
     2161 ctcagaggcc ggcgtgaccc tcaagacaat tcatcaagca aaaggcgagg aataccggcg
     2221 ggtagtagtt atgtacttgg cgcaagggca ctttcccccg gctcaggcca ctgacatgga
     2281 ggaagagagg cggttgttgt acgttgccct tactagagct gaggaggaag ttaccgtcat
     2341 aggcgagccc ggctcgctgt taatgacaga gttgaaggaa agtctgggaa ccgatgggct
     2401 ggtgtttaat tggagtgggc ggctcgactc tcccacacag ggcgaggcgg gtctttacga
     2461 agagtgtttt gaagaggatg tggcctattc gaacaccgaa cccatggaga tcctgcaaga
     2521 ctcggcatcg gatcgttcgg atgcaatgac tgtggcagag actggactca tggagatgtt
     2581 cgaagacttc atcaaacccg actaataatc atcagattgc tgatgatggg aggcatgact
     2641 tgtggaggca gtcgaactca cagaggatca gcgtcagctt gttgagtata gggtggacaa
     2701 gaatctcttg atcagaggtg aaccgggaag cggcaaaacc acagtacttg ctgccagggc
     2761 ggcgcacatg gacagcgcca tgttctccgg gtcggttctc gtgattgcct ataatagggc
     2821 gctagcaggg tacatccgta ggctgctcgg cgagtttggc ggttccgcga agattgatgt
     2881 agataccttt cacggttggg cgatgacgtt cgccgattgc cttggctatg gacctgcgcg
     2941 aatcgtgcaa cggaccgagc gtgatgccaa actagaggag attctgcggc aaatgggtcc
     3001 gaagtggggg cagaatcacc ttctaggaga acccactcaa ttctggtcgc aggaggttga
     3061 gtggatcatg ggccagggca tcagctccct ggataaatat atgaacgccg taagaacggg
     3121 cagaggcacg gcggtgcaag ttaggggcga ggacagacaa cttgtttggg acgtattcac
     3181 cgcctaccgt gactggctgg cacatgataa tgcatgtgac atacacgact tcggaggaat
     3241 ggtgcggcgg gcagtgctga aggcagaccg caagtatcca gcagacctgg cttacgacca
     3301 catactcatc gatgaggtgc aggacttcga cttatcctgg ctgctcgcct tggcgccaat
     3361 ggcacgcgtg agtgtcacgc ttgcaggaga tctcgggcag aggatatatc gtcgatcgtt
     3421 tacgtggagg gctgctggca tagacatttc tgcagccagt tcccgccagc tgcgaggttc
     3481 tcaccgcaca acccgcgaga taatgcaggt ggccatggtt atttcaacaa acacggatct
     3541 taagggcgac gaagacttcg tagcaccttc gcttcccgat cgggtgggac cgagagtttc
     3601 acggataaag cggagtggcg atagaatagc tgagaaagcc gcagtttctc gagtgactga
     3661 gctagcacgt cgctatcgag gcgattccat ctgcctggca gtcccgttca acgcaaggct
     3721 cagacgcctc accgatgagc ttatatcagc cggagtgaga gctgtcaatg caagtggcga
     3781 tgctcttgca catttggcag aggggacagt tgcagtaact acctatcatc aactgaaagg
     3841 gctcgagttc gaccacgtca tccttacagg cctcgatgat cacacaatgc ccggctttta
     3901 ctttaggatt gaagattctg aaagcgagag tgaaaaagaa cagtttttgc gccgtctcgt
     3961 atacatggct atgactcgtg caaggaaatc cgttacgctg gtcggttcaa ccccattctg
     4021 ccggttcttc aatgatgtgc caccagatta catcgaggag atatgacgac ggtgagccaa
     4081 cctggtggct actgcggggc atgatggcaa tccttgttta aatgaacata ctcttgcata
     4141 cacagatggg atcccgctag gtatcgatgt cgcctgctat gggtcgagca agggaaatgc
     4201 aacagggctt gcagcgataa tcacaggttg tgcaatacca tccatcagtc tcagccggcc
     4261 cggagcattg aaagcgcaga tatgggactt gtgatctgct gacgcaagca agtcttaaac
     4321 ctactgaaaa gcggtgtcag ggatcaccct tgcaaaacca tttccctgtt gccatcatcg
     4381 ttgcgcctga agacgagcca aactgagcgt aacttcggtt tcttcctctc aggcgtcttc
     4441 tcgagtctgt ctgcaggctt ggggaacgac
//
LOCUS       DAOSAI010000199         4411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12480_length_4411_cov_4.460055, whole genome shotgun sequence.
ACCESSION   DAOSAI010000199 DAOSAI010000000
VERSION     DAOSAI010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12480_length_4411_cov_4.460055"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(357..2444)
                     /gene="fusA"
                     /locus_tag="PLM83_07215"
     CDS             complement(357..2444)
                     /gene="fusA"
                     /locus_tag="PLM83_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOK71228.1"
                     /translation="MKKYKVDQLRNLAVVSHGGAGKTTLAEAMLYLSGGVDRFGKVDE
                     GTSTMDYDPDEVKRKISINASVAPVEWKDHKINFIDTPGYSDFVGEVVGALRVADSVL
                     VAVDAVSGVEVGTELMWRRVEEYDLPRMIVISRMDRENANFNKAVESLTEIFGNRVAV
                     AQIPIGEGDKFRGVVDLIKMRAYVWEDASGKKFVETDIPADLADIAEEHREKLVEFAA
                     ESDEELLDKYLEGEALTEDDIKAGLAKAVLSRSAYPVFCISGMKLIGIQPLMDAIISL
                     CPSPVAVGPVKGIVPNSEDTIERKPEETEPFSALVFKTMADPFVGKITMFRVYSGVVK
                     SDSQVYNASRDKTERFGQIFLTKGKQQVAVSEVGPGDIAGVAKLADTTTGDTLADKDN
                     PIILPKIQFPSSVLSMAVKPKSKGDEDKISQGLARLMEEDPTLKYDKDAESSELVVSG
                     MGEVHLDIMADRLKRKFGVEVTLEEPKIPYRETIKNTVKVEGKHKKQTGGRGQYGHVW
                     IEMSPLPAGSGFEFEDKVFGGAVPRQFIPAVEKGLREALPEGGVLAGFPLIDIKCSLY
                     DGSSHPVDSSEMAFKIAAQLALKKGCQDANPIILEPILKVEVTVPEEYMGDVMGDFNK
                     KRGRILGMEPEGRYQVVKALVPMSEMAKYSIDLRAMTGGRATFTTEFSHYEEAPANIA
                     ARVIEEAKKEKEA"
     gene            complement(2551..3789)
                     /locus_tag="PLM83_07220"
     CDS             complement(2551..3789)
                     /locus_tag="PLM83_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71229.1"
                     /translation="MRRSSCATRHKALPCVLAFTMAVFFCAPWQAAGVSAANDSLVLK
                     VALDSESLPKLTATLSHKAARFSSTVKSSTEGTHEFKLEAKMAEDISHLRQSATLSIS
                     GLLAAQGDASCWRQLSSTTGVVVDRKASPWRPEGKLSITVREYPLNAARDYSEGTVRV
                     KAGRTWSQPYAVALKAEATAGHKEMPGNPKWTANYWESSATAQSPLGSECKGSITVSA
                     KGRAYPEAEHKSYTRASCKLEGVWTPSKVHSIDCSLTAAATVREKDRSCDKTDTAISA
                     RWSIEPSRHWSADIKGSLSSSIEPNRADAGRPVRATGGVTLTCVPADIWRLTAAGDLS
                     LGHTLGENEDGDEEIVGIQSGQSGKLKMGLSFAAKLGPVNGVTLDGKLNLKRESEHAK
                     DRWEHSTWAPSAEIQASMKF"
     gene            complement(3942..>4411)
                     /locus_tag="PLM83_07225"
     CDS             complement(3942..>4411)
                     /locus_tag="PLM83_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HOK71230.1"
                     /translation="VQRINPRAQIIDAASPVTVEDPEMIRGKRVLVVEDGPTLTHGGM
                     GYGAGVVAARQFGAGGLVDPRPFAVGSIVETLERFHHLEPLLPAMGYSKKQIDELETT
                     INRSDAEVVLIGTPIDLRKVLKIDKPAVRVRYELEQESGPDLRDIVCSKFAKE"
BASE COUNT          914 a         1372 c         1058 g         1067 t
ORIGIN      
        1 cctttgttca aagcaagcat ccctccgaca tgcagccgat agaatgcccg accagggccg
       61 tatagccctt ttgccgtcat gggcgagccg gctcagatac tcacttcgat ctttcaagct
      121 ataacgggca gtcccgattg acgctacttt tgcctgcatg cctcactcag gctgcagcaa
      181 acctggttca cgccaaccgc tccggggcga ccttcgcgcg gacagaggcc aggacgcctt
      241 ccacccggtg ggcatccctc tcttgcagct taaccgcact actcctccct ttcatcgcgt
      301 ttgccattat cattttcaag tatagcatgc gcacactctg ccgcgcaggc agattgctac
      361 gcttccttct cctttttcgc ctcttcaata acgcgggccg cgatgttcgc cggagcctcc
      421 tcatagtggg agaactccgt agtaaacgtg gcgcgcccgc ccgtcatggc cctgaggtcg
      481 atagagtatt tggccatctc cgacattggc acgagagcct ttaccacctg gtatctgcct
      541 tcgggctcca taccgaggat ccgtccgcgc ttcttattga agtcgcccat gacatcgccc
      601 atgtattcct caggcaccgt tacctctacc ttcagtatag gctcgaggat tatcgggttc
      661 gcatcttgac atcccttctt gagcgccaac tgggcagcga tcttgaatgc catttcagaa
      721 gagtcgacag gatggctcga accatcatag agcgagcact taatgtcgat caatgggaaa
      781 ccggccaaca cccctccctc gggaagagcc tctctcaagc ccttctccac cgcaggaatg
      841 aactgtctcg gcaccgcgcc tccaaacacc ttgtcttcga actcgaaccc gcttcccgcc
      901 ggcaggggcg acatctcaat ccatacatgt ccatactggc cacgaccgcc ggtctgcttc
      961 ttatgcttcc cttcgacctt cacggtgttc ttgatcgtct ctctgtacgg gatcttcggt
     1021 tcttcgaggg tcacctctac gccgaacttc ctcttgagcc tgtcggccat gatgtcgagg
     1081 tgaacttcac ccattcccga aacgacaagc tccgaagatt ccgcatcctt gtcgtacttg
     1141 agcgtgggat cttcctccat aagcctcgcg aggccctggc ttatcttgtc ctcgtcgccc
     1201 ttgcttttgg gtttcacagc catcgacaga accgaactgg ggaactgaat ttttggaagt
     1261 atgatcggat tgtccttgtc ggccagcgta tcccctgtag tcgtatcagc cagcttcgct
     1321 acgcctgcaa tgtcgccagg gcccacttcg gagacagcca cttgctgctt gcccttggta
     1381 aggaatatct gcccgaaccg ctccgtttta tcgcggctcg cgttgtaaac ttgcgagtcc
     1441 gacttcacaa cccctgaata gacacggaac atggtgatct tgccgacgaa cgggtccgcc
     1501 atcgtcttga agaccaatgc cgagaagggc tcggtctcct ccggcttgcg ctctatcgtg
     1561 tcttctgagt tcggcacaat gcccttcacc ggtccaaccg ccactggcga cggacagagc
     1621 gaaattatcg catccatcag aggctgaatc ccaatcagtt tcatgccgga tatgcaaaac
     1681 acgggataag ccgaacgaga aagcacggcc ttggcaagtc ctgccttgat gtcgtcctca
     1741 gttagagcct cgccctccaa atacttgtcg aggagctcct catcactttc agcagcaaac
     1801 tcgaccagct tttcgcggtg ttcctctgct atgtcggcta ggtctgcggg gatatccgtc
     1861 tcaacaaact tctttcccga tgcatcctcc cacacataag ccctcatttt gatgaggtca
     1921 acaaccccgc ggaacttatc gccttcccct atgggaatct gcgcaacagc aaccctgttg
     1981 ccaaagattt ccgtcagaga ttcgacggcc ttgttaaagt tggcgttctc ccgatccata
     2041 cggctgatca ctatcatgcg cgggaggtcg tattcctcaa cgcgcctcca catcagctcc
     2101 gtaccgactt cgactccgga gactgcatct acagccacca agacggaatc ggccacgcgc
     2161 aacgcgccca caacctcacc cacaaaatcg gagtagccgg gagtgtctat gaagttgatc
     2221 ttgtgatcct tccattcgac aggagctacc gaggcattaa tggagatttt ccgcttgacc
     2281 tcgtcgggat cgtagtccat cgtggaagtt ccttcatcta ccttgccgaa cctatcaaca
     2341 ccgccgctca aatatagcat ggcctcggca agagtcgttt tgccggcgcc gccatgcgat
     2401 acaacggcaa ggttgcgcag ttgatccact ttatattttt tcaagcgatc tcctccctta
     2461 agccttatcg cagccctgca attaactgcc tgtttgcaac agatactcac tatagtatct
     2521 tacacttaac ccagcactga ggcaatagcc ttagaacttc atagacgcct gaatctccgc
     2581 cgaaggggcc catgtgctgt gttcccatcg atcttttgcg tgctcactct cgcgcttcag
     2641 gttgagtttt ccgtccaaag ttactccgtt tacgggaccc aactttgctg caaatgaaag
     2701 ccccatctta agcttgccgc tttgccccga ctgaatgccg acgatctcct cgtccccatc
     2761 ctcgttttcg cctaaggtat ggcctaatga cagatctcct gcggcagtca gcctccagat
     2821 gtccgctggc acgcaagtca gggtaactcc tccggttgcc cgcaccggcc tgccggcatc
     2881 ggccctattg ggctcaatcg acgatgaaag cgaccctttg atgtctgcac tccaatgtcg
     2941 agaaggctca atcgaccatc tagccgaaat cgctgtgtcg gtcttatcac aagatctgtc
     3001 cttctcccgg accgtagccg ccgctgtgag gctgcagtcg atcgaatgta ccttagacgg
     3061 ggtccaaaca ccctcaagtt tgcatgacgc gcgggtatat gatttgtgct cggcttcggg
     3121 gtatgcccgg cctttggctg atactgtgat tgaacctttg cattccgaac ccagaggtga
     3181 ttgcgcggtt gcagacgatt cccagtagtt cgcagtccac ttaggattgc ccggcatctc
     3241 tttgtggccc gcggtcgctt ccgctttcaa tgcaacagca tacggttgcg accatgtgcg
     3301 cccagccttg accctcactg tgccttcgct gtagtccctg gccgcattga gcggatattc
     3361 tctcacggtt atggaaagct tcccctcagg ccgccacgga ctcgctttcc tatcgacaac
     3421 aacgccggta gtggaggaaa gctgacgcca acaggaagca tccccctgcg cagcaaggag
     3481 gcctgagatg ctcaaagttg cagactgccg gagatgcgat atatcttcag ccattttggc
     3541 ctccaatttg aattcatgcg ttccttctgt ggatgatttt actgtcgaag agaagcgagc
     3601 ggctttgtgg gacaaggtgg cagtcagctt cggcaaggat tccgagtcga gcgcgacttt
     3661 gagcacaagg gaatcattgg cggcagacac gcccgccgcc tgccatgggg cgcagaagaa
     3721 caccgccatc gtaaatgcca gcacacatgg gagagcttta tgcctggtgg cgcaagagct
     3781 ccgacgcatc aaccatccct cctgtcagtg ttgattctga caggattata ccattttaag
     3841 gctacaatgt aaactatgtg atactatatg ggcttgtttc catatctgcc atagcttgta
     3901 cggccccggc atgccatttt ggccggggcc cgcccaaaca tctactcctt agcgaacttg
     3961 gaacacacaa tatccctgag gtcggggccg gactcctgtt cgagctcgta cctgactctc
     4021 acggcaggct tgtctatctt gagaaccttc cggagatcga tcggagtgcc aatcagcacc
     4081 acttcggcat cagatctgtt gatggtggtc tcaagctcat ctatctgctt tttgctgtat
     4141 cccattgcag ggagcaacgg ctcgagatga tggaacctct caagagtttc gacgatgctg
     4201 cctacggcaa acggcctcgg gtcgacaagc ccgccggcac cgaactggcg tgccgctacc
     4261 actcccgcgc cataacccat tccgccatgg gtgagcgtcg gaccgtcctc tactaccaac
     4321 acacgcttac cgcggatcat ctcaggatct tccactgtta caggcgaagc cgcatctatg
     4381 atctgagccc tcgggttgat cctctgcacg t
//
LOCUS       DAOSAI010000200         4409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12487_length_4409_cov_3.426045, whole genome shotgun sequence.
ACCESSION   DAOSAI010000200 DAOSAI010000000
VERSION     DAOSAI010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12487_length_4409_cov_3.426045"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..333
                     /locus_tag="PLM83_07230"
     CDS             34..333
                     /locus_tag="PLM83_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71231.1"
                     /translation="MTQVFSEYGNDFSWETRTQSEDRIHRAGQMHNCHIIDIVCADSI
                     DMRIQRCLTNKEQLVDSFRKEIDRTKDKTSLGRWIDGGEEEIPEPGCVSGGEGAA"
     gene            275..1573
                     /locus_tag="PLM83_07235"
     CDS             275..1573
                     /locus_tag="PLM83_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HOK71232.1"
                     /translation="MARKKYLNQDVYQAAKARLEFLFDEFEHIYFSFSGGKDSGVMME
                     LALEVARERDRLPLHALLIDLEAHYREHIEYSLRFCDRPEIKPYWICLPISLRNAVSQ
                     FEPKWVPWDDAQRDIWVRPIPDRPYVIHQDNMPTEWTWFHKGMEFEELILYFADWFAD
                     VHQADKTACVVGIRADESMNRYRTIVNDRKTRYADQGWTTLVKGLRNPVYNAYPIYDW
                     RVEDIWAYVGKTGCDYNKIYDYMYKTGRSIHDMRICQPYGDDQRKGLDMFQECEPETW
                     FKVVQRVSGANFGAIYRGGTILGNIKVELPPGHTWESYAKLLLNSMPPYLKEHYIEYF
                     NTFFRWWDRFGGGRTEHQTVVTDEDKYDEMGIYYSDKIPYDGYQGDDVPDSPKWRQPL
                     WKRICKVLLKNDYWCKELSFSQVKGEYERLESLKEKYRDI"
     gene            1586..2191
                     /locus_tag="PLM83_07240"
     CDS             1586..2191
                     /locus_tag="PLM83_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71233.1"
                     /translation="MEIHDIGGHRLTLAELGQDDCAGVLDRRAHVVYNAPPNPDTINW
                     WRGYKQVSYTYQDFLDALFALYDQADADEYYIETGKVHDSVVDHLRGRGLYIQERPIL
                     YSAPLNGHGLGMAKRRIPCTLVVASRQPVPLPAGEYSHETIADALSRRQPGETVFDPC
                     IGKGLALRAAIARGMRCVGIESSPERLKCCIEYATREAERC"
     gene            2230..2820
                     /locus_tag="PLM83_07245"
     CDS             2230..2820
                     /locus_tag="PLM83_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044260 - cellular macromolecule metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOK71234.1"
                     /translation="MRDTLGAMDTEERIEAINELRGVLHELSPFRAEPVDYVQWVPAE
                     QVTANDYNPNAVAPPEMRLLYISIREDHFTQPIVSARDAENNHYIIVDGFHRNRVGKE
                     YRDIRERLHGYLPIVTTDKPLAERMASTIRHNRARGKHAVSPMSDLVAALYRDGWPDD
                     RIAKELGMELDEVLRLKQITGLPEIFANREYSKAWE"
     gene            2844..3308
                     /locus_tag="PLM83_07250"
     CDS             2844..3308
                     /locus_tag="PLM83_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71235.1"
                     /translation="MNFERIAVGEPLAGAREAQLRNQTLMLLEWGNSGYTLYVALPNM
                     TEQEAEIIKHNKVKVRMLGEADCLLPVWQFIDSQLFGETPFDPTLYLPYYLDCKEDLY
                     QKTVDKNAREHYTDIRTMIIHTTEPGGDTNEYWTRHKAGQKRARADPAAASK"
     gene            3642..3806
                     /locus_tag="PLM83_07255"
     CDS             3642..3806
                     /locus_tag="PLM83_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71236.1"
                     /translation="MEIISPYHQILSVMMMFAVALAIVAFGLQVAELVAQRKKRKAPT
                     DQPAERQSAR"
     gene            3908..>4409
                     /locus_tag="PLM83_07260"
     CDS             3908..>4409
                     /locus_tag="PLM83_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HOK71237.1"
                     /translation="MMTLAEELDSILDEAYEPIADAAYAVLSAGGDPAALLGAEDEQA
                     RQFRITDEGMADWAIRKIAKARKEFDEAGKVADAQIARISDWLARKKRDMERTEEFFG
                     GLLRAYYQPQHEADPKRRKTFHLPSGQVQFRAQQPEYKRDDEQLLKWLKANGRETYIV
                     TKESPAW"
BASE COUNT         1120 a         1102 c         1315 g          872 t
ORIGIN      
        1 cgctaatagc aaaggaggca atgaccttga gtcatgacac aggtgtttag cgaatacggc
       61 aacgatttca gttgggagac tcgcacgcaa tcagaggacc ggattcaccg tgcaggccag
      121 atgcacaatt gccatatcat agacattgtt tgcgctgaca gcattgatat gcgcattcag
      181 cgctgcctga caaacaaaga gcagttagtg gactccttcc gcaaggagat agatcgcacc
      241 aaagacaaga ctagtttggg gaggtggata gacggtggcg aggaagaaat acctgaacca
      301 ggatgtgtat caggcggcga aggcgcggct tgagttccta ttcgatgagt ttgaacatat
      361 ctacttctcc ttctctggcg gcaaggactc cggagtaatg atggagctgg ccctggaagt
      421 agcgcgtgaa cgcgatcgtc tgccgctcca tgctttgctc attgatttag aagcacacta
      481 cagagaacac atcgagtatt ctctgcggtt ctgtgatcgg cctgagataa aaccatattg
      541 gatatgctta cctatctctc tgcgaaatgc tgtttcccag ttcgagccca aatgggtgcc
      601 ctgggacgat gcacagcgtg acatatgggt gcgaccaatt ccagacaggc cgtatgtcat
      661 tcatcaagac aacatgccaa ctgagtggac gtggttccac aagggcatgg agtttgagga
      721 gctaatcctt tacttcgctg actggttcgc ggatgttcac caagcagata aaaccgcctg
      781 tgtagtaggc attcgtgccg acgagagcat gaacagatat cggactattg taaatgaccg
      841 caagacacgc tacgcagacc agggatggac cacgttagtc aaagggctgc gcaatccagt
      901 atacaacgcg tatccaatct atgattggcg tgtcgaagat atatgggctt atgtgggcaa
      961 gacaggctgc gactacaaca agatctacga ctacatgtac aagaccggca ggtcaattca
     1021 cgacatgcga atatgccagc cctatggcga tgaccagcgc aaggggttgg acatgttcca
     1081 ggagtgcgag ccagaaacat ggttcaaggt ggtgcagaga gtctccggag ccaactttgg
     1141 cgcgatctac cggggcggga cgattctcgg caatatcaaa gtcgagctac ctccggggca
     1201 tacctgggag agctatgcca agctgctcct gaacagtatg cctccgtatc tcaaagagca
     1261 ctacattgag tattttaata ctttcttcag atggtgggat cggtttggag gcgggcgcac
     1321 cgagcatcaa acggttgtca ctgacgagga caagtatgac gaaatgggta tctactactc
     1381 agacaagatt ccatatgacg gataccaagg cgatgatgtg cctgatagcc ccaagtggcg
     1441 gcagccgtta tggaagcgga tctgcaaggt gctcctgaag aacgattact ggtgtaagga
     1501 actatcgttc agccaagtga agggagaata tgagcgacta gagtctctga aagagaagta
     1561 tcgtgacata tagggaggca ctgctatgga gatccacgat atcggtggcc atcgcttaac
     1621 gcttgcggaa ctgggccagg atgattgtgc tggagttctg gatcggcgcg cccacgtcgt
     1681 ttacaatgct cctcctaacc ctgatacgat caactggtgg cgcggatata aacaggtttc
     1741 atacacatat caggactttc tcgatgcatt gtttgcgctc tacgatcagg cggacgccga
     1801 tgagtactac atagagacag gcaaggtgca tgattccgta gtcgaccacc tgcgcggccg
     1861 agggttgtac atacaggagc gccccatcct ctactcggcg cccctgaacg ggcatgggct
     1921 gggcatggcc aagaggcgaa taccctgtac gttagtggta gcatcgaggc aacctgtgcc
     1981 gctaccggcc ggtgaatact cgcatgagac gattgccgat gctctatcgc gccggcagcc
     2041 aggcgaaacg gtgttcgacc cgtgcatcgg caaaggcttg gctcttcggg cagccatcgc
     2101 gcggggtatg cggtgtgtcg ggatagagtc gagcccagag cggttgaagt gctgtatcga
     2161 atatgcgaca agggaggcag agcggtgctg agtctatttg catcaacatt ggatgatatc
     2221 aaggcctcaa tgcgcgacac actgggcgcc atggacacag aggagcgcat agaggccatc
     2281 aacgagcttc gcggggtgtt gcatgagcta tcgccgttcc gggcagagcc tgtggattat
     2341 gtccagtggg ttccggcaga gcaggtgacg gccaacgact acaatcctaa tgctgtcgcc
     2401 ccgccggaga tgcggttgct ctatatcagc atccgtgagg accacttcac acaacctatc
     2461 gtgtccgctc gcgatgcaga gaacaatcac tacatcattg tcgacggttt ccatcgaaac
     2521 cgcgtgggca aggagtaccg tgacatcagg gaacgtctgc atggttacct gcctatagtt
     2581 acgacagaca agcccctcgc cgagaggatg gctagcacca tacggcacaa tcgggccagg
     2641 ggcaaacatg ctgtgtcgcc catgtcagat ctggtagcag ctctataccg cgatgggtgg
     2701 ccagatgaca gaatcgctaa ggaactgggc atggaacttg atgaagtatt gcggctcaaa
     2761 cagatcaccg gactgcctga gatattcgcc aatcgcgagt attccaaggc gtgggaatag
     2821 gggaaggaag agaggttaat agaatgaact ttgaacgaat tgcagtcgga gagccattgg
     2881 ctggtgctcg ggaagcccag ctacgcaacc aaactttgat gctccttgag tggggtaata
     2941 gcggttacac gctgtatgtc gccctgccca atatgactga gcaggaagct gagatcatca
     3001 aacacaacaa ggtcaaagtt cgaatgctcg gggaggcaga ttgcctgttg ccagtttggc
     3061 agttcatcga cagtcaactg ttcggtgaga cgccatttga cccaacattg tatttgccct
     3121 attacctaga ttgtaaagag gacctgtatc aaaaaactgt tgacaagaac gcaagggaac
     3181 attatactga tatcagaacc atgataattc acacaacaga accgggtggt gatacgaatg
     3241 agtattggac gcgccataaa gcaggccaga agcgagcgcg ggctgaccca gcagcagcta
     3301 gcaaataggg ctggcatttc catatcgtcg ctgtactgca ttgaatctga cacaaacagc
     3361 ccgtccgtta ggacactcgg caagatcgcc gcagccctag aagtgccggt ttctgcactg
     3421 tttgaagaga cgcagagtct ggacgcagct gcgggcgagt aggcccgcat gagacggagg
     3481 tggacaagca tgtgcaggag ggagtgtttg atacctggtg aggaatgcga gtacaaagca
     3541 cagtgccagg cggcggaaac acgggccgag gcggagagag ccgtggtcca ggcgatcgca
     3601 agggcccgca aggtcctaca gagacaggag gcggggatgg agtggagatc atcagcccat
     3661 atcaccagat actcagcgtg atgatgatgt ttgcggtggc gctggcgata gtggccttcg
     3721 ggctacaggt agctgagctc gttgcccagc gcaagaaaag aaaagcaccg actgatcagc
     3781 ctgcagagcg acagtcggcg cggtgaagcg atttccaaaa gggcgcttct acatctctat
     3841 tataccagtt ctgcgcagct gtgcaactgg gtggaggcgc cctccgagat aactgagagg
     3901 gggacacatg atgactctag cagaggagct cgacagcatc ctggatgagg catacgagcc
     3961 aatcgcggac gcagcctacg cggtgctcag cgcgggcggc gatccagcgg ccctactcgg
     4021 cgctgaggac gagcaggcgc ggcagttccg catcaccgac gagggcatgg ccgactgggc
     4081 catacgcaag atcgccaagg cccgaaagga gttcgacgag gcgggcaagg tggccgatgc
     4141 gcagatcgcc cgcatcagcg actggctggc ccgcaagaag cgcgacatgg agcgcaccga
     4201 ggagttcttt ggcggcttgc tcagggcgta ttaccagccc cagcacgagg cggaccccaa
     4261 gaggcgcaag accttccacc tgccgagcgg acaggtgcag ttccgggcgc agcagcccga
     4321 gtacaagcgc gacgacgagc agctactcaa gtggctcaag gccaacgggc gcgagaccta
     4381 catcgtcacc aaagagtcgc ccgcctggg
//
LOCUS       DAOSAI010000201         4399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12523_length_4399_cov_3.520028, whole genome shotgun sequence.
ACCESSION   DAOSAI010000201 DAOSAI010000000
VERSION     DAOSAI010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12523_length_4399_cov_3.520028"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="PLM83_07265"
     CDS             complement(<1..1309)
                     /locus_tag="PLM83_07265"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HOK71238.1"
                     /translation="MNSLSFPEDFLWGVATSGHQVEGNNTASDWWAWEPGKIEGGLTS
                     GRACDAWNRYEEDYATMKSMGLNAYRMGIEWARVEPRPGMWDDAVFAHYEAMMESLSE
                     KGIAICLTLYHWVLPKWVADQDGWENPATCDAFLRFVQEVVNRLGKYPLLWCTLNEPN
                     VYTMFSYVTGEFPPEKRSLRAARRVTRHLLTAHAGAYSIICEGDSKSADPNSRPLIGI
                     AHNMTDFAPASHSPFDRAIAKYQSKSWNSSYIEALVTGRMPGPFGDGKPIPGLARSLT
                     YMGLNYYTRRTVAFRPNGISNAFSQEVFPIGTELNDFGWAVCPEGIYNCLVELGKLRI
                     PVYITENGVADANDSMRPRYIVEHLREVWRAIGSGVDVRGYFYWTFQDNFEWRAGYTQ
                     KFGLFELDPTDPNLQRTPRRSAEMYAKIAKANGITESISTKYLG"
     gene            1465..2616
                     /gene="mtnA"
                     /locus_tag="PLM83_07270"
     CDS             1465..2616
                     /gene="mtnA"
                     /locus_tag="PLM83_07270"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOK71239.1"
                     /translation="MMGKTDMDKGDDLLSTTSSGRKGSGRPVRLEPITWDGNKRVVRL
                     IDQTLLPTELRYIEISAMDAMCEAIAELRVRGAPAIGIAGAFGVYLGVANRSFSSEKE
                     FFEVLDGVCSEVACVRPTAVNLAWAVKRVKRVAGEVADSAHSRGERADPEALVEAILS
                     EAIIMIDEDNKVCRAIGEYGASLIPDGASILTHCNAGALATAGWGTALAALYVAREQG
                     KEFRVWADETRPLLQGSRLTAFELMEAGIDVTVICDNMAASLMGRGEIDLVILGADRV
                     AANGDVCNKIGTYGVACLAHMHDIPFFVACPTSTFDLSLASGAEIPIEQRNPREVTHG
                     FGKQTAPDGVKVYNPAFDVTPHNLMSAIITERGIVRAPYGPGLAAIVRD"
     gene            complement(2600..3604)
                     /locus_tag="PLM83_07275"
     CDS             complement(2600..3604)
                     /locus_tag="PLM83_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOK71240.1"
                     /translation="MNRLYPLSFIPLFKEKIWGGRRFESWFPVIPDGPIGEAWVLSAH
                     PHGLTPTENGYLANKTIPELLTEYGEDVIGKAWADQDYSDPPQCCKFPLLIKLLNSND
                     DLSVQVHPGEEYAATHLEASAKSEMWLVLEADPGTSIVYGLKEGIGPREFAEAAKSGS
                     VMDYLNEVQVSAGDVIAIPPGTIHALGAGLVVAEVQQSSDTVYRIWDYNRPGLDGKPR
                     ELHIDQALEVIDYSRPPEITQPKMASPNSGVSIGSFAGFDVQLARCCGRWERRPSPSS
                     FTAFLFLGGTGEVAWGEDERAHISVRPGSSVLIPASCPDYALRSVSSDLVFLEVSRAL
                     "
BASE COUNT          909 a         1088 c         1350 g         1052 t
ORIGIN      
        1 tccccaggta ttttgttgaa atgctctccg taattccgtt ggctttggct atcttcgcat
       61 acatctccgc acttcttcgg ggcgtccttt gcaggttcgg atcggtcggg tcaagttcga
      121 agaggccgaa cttctgtgtg tatcctgcac gccattcgaa gttgtcttgg aaggtccaat
      181 aaaaatagcc gcgcacgtca acgccgctgc caattgcgcg ccacacctcc cggaggtgtt
      241 caacgatgta gcgcgggcgc atcgagtcgt tggcatcggc cactccattt tcagtgatgt
      301 atactgggat acgaagtttc ccgagttcca caaggcaatt gtatatcccc tctgggcaga
      361 ccgcccagcc gaaatcgttg agctctgtgc ctatcgggaa gacttcttgg ctgaaggcgt
      421 tggagatgcc gtttgggcgg aaagctacgg tccgtctggt gtaatagttg agtcccatgt
      481 atgtgaggga tctagccaaa cccgggatag gttttccgtc gccgaatggg cccggcattc
      541 gtcctgtgac tagggcttct atgtatgagc tgttccagga cttggactgg tatttcgcta
      601 tggcgcgatc aaatgggctg tggctggcag gggcaaagtc cgtcatgttg tgggctatgc
      661 caataagtgg cctgctgttc gggtcggcgc ttttgctatc gccctcacag atgattgagt
      721 aggcgcctgc gtgggcggtc aagaggtgac gggtgactcg gcgggcggct ctgagggatc
      781 gcttttcggg cggaaactcg ccggtcacgt aagagaacat agtgtagacg ttgggttcat
      841 tcaaagtgca ccaaaggagg gggtacttgc cgagccggtt tacgacctct tgcacgaagc
      901 ggaggaatgc atcgcatgtt gcgggatttt cccacccgtc ctggtcagcc acccattttg
      961 gcagcaccca gtggtaaaga gtgaggcaaa ttgcgattcc cttttcggac agagattcca
     1021 tcattgcttc atagtgggca aagacggcat catcccacat gccggggcgc ggctccactc
     1081 gggcccattc aattcccatc ctgtaggcgt tgagccccat ggacttcatg gtcgcgtagt
     1141 cttcctcgta tctgttccac gcgtcgcagg cccggcctga tgtgaggccg ccttcgatct
     1201 tcccgggttc ccatgcccac cagtcgcttg cggtgttgtt gccctctact tgatgtccgg
     1261 atgttgctac gccccacaag aaatcttcag gaaaggaaag gctattcact gatgccacct
     1321 cctggtgtgc agattcgcct gatgcgtcca tactcctcca tgcgtgcgac tgcaaaccgc
     1381 catggcatag ggcgcatccg gcgaccttat gaaatgcgga tatggcggcg ggttggcagg
     1441 aaagaccgag gatgtccgag aaagatgatg ggaaagacgg acatggacaa gggggatgat
     1501 ctgttgtcaa cgacatcttc ggggaggaag ggcagcggta ggcctgtccg tttggagccg
     1561 atcacatggg atggcaacaa gcgagtggtc cgcctcatcg accagactct ccttccaact
     1621 gaactaagat acatcgaaat atcagctatg gacgcgatgt gcgaggctat agcggagctc
     1681 agagtgcgcg gggcgccagc gattgggata gcgggcgcgt tcggtgtcta cttgggggtt
     1741 gctaacagga gcttttcgtc tgaaaaagag ttttttgagg tcctcgatgg tgtctgctcc
     1801 gaggtggcgt gcgtgcggcc gacggcagtg aacttggcat gggctgtcaa gcgggtgaag
     1861 cgcgtggccg gggaggttgc ggatagcgcc catagtcgcg gagagagggc ggacccggag
     1921 gcacttgtgg aggctatcct gagcgaggcc ataatcatga tagacgagga caacaaggta
     1981 tgcagggcga taggggagta cggtgcgagt ctcatacctg acggcgcatc tattctgacc
     2041 cactgcaacg ccggggccct ggccactgca ggctggggga cagccttagc ggctctttat
     2101 gtggcccggg agcaaggcaa ggagtttaga gtctgggcgg atgagaccag gccgcttttg
     2161 cagggatcgc gtcttacggc ttttgagctg atggaggcgg ggatagatgt aacggtcata
     2221 tgcgacaaca tggctgcctc cttgatgggc aggggcgaga tcgacttggt cattctcggg
     2281 gccgaccgtg ttgccgccaa tggggatgta tgcaacaaga tcgggacata tggagttgct
     2341 tgtcttgcgc acatgcacga cattccgttc ttcgtggcat gccccacgtc cacctttgac
     2401 ttgtcgcttg ccagcggagc ggagattccc atagaacaaa ggaacccgcg ggaagtgacc
     2461 cacgggttcg gaaagcagac tgctcctgat ggtgtgaagg tttataaccc ggctttcgat
     2521 gtgacaccgc acaatctcat gtcggcgatc atcacggagc gggggatagt tcgcgcgcca
     2581 tacgggccgg ggcttgctgc tatagtgcgc gactgacttc caagaatacg agatcggacg
     2641 ataccgaccg gagtgcataa tcagggcagc tcgcaggtat caatactgat gatccggggc
     2701 gtacggaaat gtgcgccctt tcatcttctc cccatgccac ctctcctgta cccccgagga
     2761 acaggaaggc agtgaacgaa gagggcgatg ggcgccgttc ccatctgccg cagcaccttg
     2821 cgagttggac gtcgaagcct gcgaacgagc ctatggatac gcccgagttt ggcgacgcca
     2881 ttttcggctg cgtaatctcc ggcgggcgtg aatagtcgat cacttctagc gcttggtcga
     2941 tgtggagctc gcgaggcttt ccgtcaaggc ctggcctgtt gtagtcccag atgcggtaga
     3001 ccgtgtcgga gctttgttgg acttctgcga ccacaagacc tgcgcctagg gcgtggatgg
     3061 tcccgggcgg tatggcaatg acgtctccgg ctgaaacttg tacctcgttg aggtaatcca
     3121 ttacggatcc gcttttcgcg gcctcagcga attcccttgg gccgatgcct tctttgaggc
     3181 catacactat tgaggttcct ggatctgctt caagaaccag ccacatttcg gatttggccg
     3241 atgcttccag gtgtgttgct gcatattctt cacccggatg cacctggaca gagaggtcat
     3301 cgttggagtt gaggagcttg atgaggaggg ggaacttgca gcactgcggc gggtcggaat
     3361 agtcctggtc ggcccaagcc ttgccgatta cgtcttctcc atactccgta aggagctcag
     3421 gaatggtctt gtttgccagg tatccgtttt ctgtaggggt cagcccgtgt ggatgggctg
     3481 aaaggaccca tgcttcccct atgggcccat cagggattac gggaaaccat gattcgaaac
     3541 gacgtcctcc ccagattttc tctttgaata aaggaatgaa tgatagcgga tagagtctgt
     3601 tcaatctcaa acacctcaca gtgctaacta catcttactg ttagggattg ggtcggtcaa
     3661 cattggatgg gttgcagctg ggggtaaaga cgtcaaagca gggtgggtgg gaaactgtcc
     3721 gcagttgatc ctaaagcact ctcagcccgt attagtcttc gcagctaagt gtctggaaaa
     3781 tggggaattc tatgctttga ctcgcaggga acaatggaag cgttggcgaa cgagaatttc
     3841 ccattttgca ctgtattgta attgggggcc tttggccatg acgtgcttgg catctcggaa
     3901 agttgtccag gaatcagcga cgatgatctt ctcgaactgg ccaggtcgca agggagatta
     3961 atcctcaata acgaattcac tgatgtatgg ctgcattcag atgcttgtaa tttctctttt
     4021 ccagtgtgct ccgctttcct gtgtgggcct tgagatccgt gatctgtgtt gatcatgcaa
     4081 ggtgttagcg aagtgcagct accaaggcat gcttggggag gctggaaaac ctatgccttg
     4141 aggtcctcta gtgatatagt tataagtggt atagtttcgg ggattatgca atggtaatgg
     4201 cattatcgtt gccctggtca acgcggtcag ggcaccgctt ctcgcacggg agcgcggatt
     4261 gagacaagca aacggggggt tagcttatgc ctaaactaga ttttctggcg caggaactcg
     4321 agtccttgca cgaacagaat ttgttcaatg tgataaggac gattgagggg ccgcagggcg
     4381 gctggattgt agtggacgg
//
LOCUS       DAOSAI010000202         4387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12562_length_4387_cov_4.021006, whole genome shotgun sequence.
ACCESSION   DAOSAI010000202 DAOSAI010000000
VERSION     DAOSAI010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12562_length_4387_cov_4.021006"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLM83_07280"
     CDS             complement(<1..364)
                     /locus_tag="PLM83_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71241.1"
                     /translation="MQGCVSEDTTVFLKDSAGRILEVALVFFNAMLDSDAGGDDLGEY
                     KILGRSGWTDLLCVARRNLWEGEQLCHIRTRLGRISLTGEHELYVRRGPVDVVVAAND
                     VKAGDALLMLERPRLGKDA"
     gene            complement(873..1118)
                     /locus_tag="PLM83_07285"
     CDS             complement(873..1118)
                     /locus_tag="PLM83_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HOK71242.1"
                     /translation="MQRASELRVKEVVSVNDGKRLGFMFDVEIDMDTGKVISFVIPSG
                     GKVLGFLGRGGELVIEWDRIRKIGLDVILVDDAGLTL"
     gene            complement(1206..1946)
                     /locus_tag="PLM83_07290"
     CDS             complement(1206..1946)
                     /locus_tag="PLM83_07290"
                     /inference="COORDINATES: protein motif:HMM:NF021093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HOK71243.1"
                     /translation="MPTTKKEIVARTITCGLVLFCFTLCAVVLAAQSDAAWGEARLAY
                     NTSNLIRLHVVAPADDSASSCLKLAVRDAILGETSSLLESAKDAHEAETRIRANLGRI
                     QRMANEVVARAGFSHSVRVTLGMEQFPAISYGQISLPSGSYLALTAAIGPAAGSNWWC
                     ILFPPLCLVDVTEGCIIEARQDGNRPIGLEFATRSLLFAPTAAEKEAALYHASRATQR
                     ATLAQLNWELPEWICRLLGLSRFDQAKR"
     gene            complement(1973..2746)
                     /gene="sigG"
                     /locus_tag="PLM83_07295"
     CDS             complement(1973..2746)
                     /gene="sigG"
                     /locus_tag="PLM83_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HOK71244.1"
                     /translation="MALNKVEICGVNTSKLPVLSSSKMRELLEAVRDGDKEARAKLIH
                     GNLRLVLSVIQRFTNRGEYVDDLFQVGCVGLIKAIDNFDLSQNVKFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLRDIAYRALQVRDKLMSETAREPTINEIAAALGIPSEEVVYAL
                     DAIQEPISLFEPIYHDGGDPIYVMDQVSDEKNTDANWLEGISIKEAMLRLSPREQTIL
                     KLRFFEGKTQMEVADEIGISQAQVSRLEKNALRQMRKSM"
     gene            complement(2911..3645)
                     /gene="sigE"
                     /locus_tag="PLM83_07300"
     CDS             complement(2911..3645)
                     /gene="sigE"
                     /locus_tag="PLM83_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HOK71245.1"
                     /translation="MRKRLESIMWAIRLFIIKAIGWFVIVEDSQVRYIGSHEMLPPPL
                     SLDEEAFLMEKLKTGDICVRSELVERNLRLVVYIARKFESTGVGVEDLVSIGTIGLIK
                     AVNTFDPEKKIKLATYASRCIENEILMYLRKTNKIRSEVSFDEPLNVDWDGNELLLSD
                     VYGTEDDLVYQALEEEVDRRLLGEALSSLSGREKRIMELRFGLSDGSEKTQKQVADLL
                     GISQSYISRLEKRILTKLRRQMIRVE"
     gene            complement(3667..>4387)
                     /locus_tag="PLM83_07305"
     CDS             complement(3667..>4387)
                     /locus_tag="PLM83_07305"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HOK71246.1"
                     /translation="LSDLGIIILSSPMSLTVALLAAGASLVIAFPGMDARELIVALFY
                     RYLLTVMAGGAATAAYSAAGGSRPLSFIASVATVMLVAEAGWGAVHRGMREGLFYVPI
                     SIRFDRYSVSLTALIDTGNLLRDPISGVPAIIVEYSAIEPILPHTIKAMLEISDGDFA
                     AAAAAIAESPWSTRFRAVPYSSVGQTRGLMVGFRPSEVRVIDGKKEISTDRAIICVHN
                     ASLCSNGKYRALLNPEILTAG"
BASE COUNT         1019 a         1282 c         1041 g         1045 t
ORIGIN      
        1 gagcatcctt cccaagtctc ggacgttcaa gcatgagaag cgcgtctccg gccttgacat
       61 cgttcgccgc cacgaccacg tcgaccgggc ccctcctcac gtatagctcg tgttcgccag
      121 tcagcgaaat acggccaaga cgcgtgcgga tgtggcagag ctgctcaccc tcccacagat
      181 tgcgcctggc cacgcaaaga agatcggtcc aacccgatct gcctaagatc ttgtattccc
      241 caagatcatc gccgccagca tcggaatcga gcatggcatt gaagaacaca agagctactt
      301 ccagaattcg gccagccgag tccttgagga aaacagttgt atcctcggaa acacagcctt
      361 gcatgtaaca tcgcctccag caggcgaaag tataacatca gtggcaccgt ctttcaaggg
      421 gcaatccccc tttactatca acatccttcg tatatatcct gcccctagat ggacccgatc
      481 aacatgtatg ttatggataa tatcggggta ataccctcat tcccggttca cgtaaggcgc
      541 acacgagcat acccagatca attatcgcct ttctagggca tcgccacctc agcctgggct
      601 ttcacccagt atactcaggg tactttcgca taacggcatt ccccaggcga tacccggaca
      661 agattttgcc ttcatcttcc cgggtctgac tcgctgaccc ttgcgagaaa tggcgataga
      721 ccgttgggcg gtctcgacag ctccttcaag ttttgatttg gaatcggtct ccaatactaa
      781 tccaatcaag agcagtattc ttgtagatgc tacgatccca acaaagggcc ccaggatcat
      841 gcattgaaga tggcgctaaa tggggacagc gatcacagtg tcagtcccgc gtcatcgacg
      901 aggattacat ctaggccgat cttgcgtatc cgatcccatt ctatgaccag ctcccccccg
      961 cgccccaaaa atcccagaac ttttccgccg ctgggtatca caaagctgat gactttgcct
     1021 gtgtccatgt ctatctccac gtcgaacatg aagcccagtc tctttccatc gttgacgctc
     1081 acgacttcct tgaccctcag ttcagatgcc cgctgtatca cgtgcgcacc tcctgaaaac
     1141 gaaaactggc cctccacata gtatgcggga gccagctgca aggtgcttgt tccagtggcc
     1201 tatcttcatc tcttagcttg atcaaacctg ctcaggccga ggagcctgca tatccactcc
     1261 ggcaattccc agttgagttg cgcaagcgta gcacgctgag tagccctgga tgcatgatag
     1321 agggccgctt ccttttcggc agccgttgga gcaaacagaa gagacctcgt tgcgaactca
     1381 aggccgatag gcctgttgcc atcttgccta gcctctatta tgcatccctc tgtcacgtcc
     1441 accaagcaaa gagggggaaa cagaatgcac caccagtttg agccggcagc cggccctata
     1501 gcagctgtaa gcgccaggta gcttcctgaa ggcaacgata tctggccgta cgatatcgca
     1561 gggaactgct ccatacccaa ggtaacgcgg acggaatgtg agaatcccgc ccgtgcaaca
     1621 acttcattcg ccattcgttg gatccgaccg aggttggccc ggatgcgcgt ttcagcctca
     1681 tgggcatcct tcgcgctctc cagcaaggaa gaggtctcgc caagaatggc gtctcgaacc
     1741 gccagcttca aacagctgct tgcgctgtca tctgccggtg ccacgacgtg aagtctgatc
     1801 agattggacg tgttgtaggc tagcctggct tcaccccaag ccgcatcaga ttgggcagcc
     1861 agaaccacgg cgcacagcgt gaaacagaat aacactagtc cacatgttat tgttctggcg
     1921 actatttcct ttttcgtagt cggcaccccc tcttgggctt atgagacccg agttacatcg
     1981 attttcgcat ctgtcgaagc gcgttttttt cgagccttga cacctgtgcc tgcgatatgc
     2041 ctatctcgtc cgccacttcc atctgggtct tcccttcaaa aaaccttagc ttgagtatgg
     2101 tctgttccct cggcgacagt ctgagcatgg cctcctttat agaaatcccc tcaagccagt
     2161 tcgcgtcggt gttcttctcg tccgatacct ggtccatgac gtatatgggg tcgccgccat
     2221 cgtggtatat tggctcaaac aaggatatcg gctcctgtat tgcatcgagt gcatagacta
     2281 cctcttcact tggtattcct aaagctgcgg cgatctcgtt tatcgtaggt tctcttgcag
     2341 tttctgacat taacttgtcg cggacctgca gggctctgta tgcaatatcg cgaagcgagc
     2401 gggatactct gatcgggttg ttgtcacgca ggtagcgccg gatttcgcct attatcatcg
     2461 ggacggcata tgtggagaat ttcacgtttt gagacaggtc aaagttatcg atggctttaa
     2521 tgagccccac gcaacctacc tgaaacagat catcaacata ttcgcccctg ttggtgaatc
     2581 gctgtattac cgagagcacc agccgcaagt tcccgtgtat cagtttggcg cgagcttcct
     2641 tgtcgccatc gcgcaccgct tcgagcagtt cacgcatttt tgagctggat agcaccggga
     2701 gtttcgaggt attcacgccg cagatctcga ccttgttaag cgccatatcg gaacaactcc
     2761 acattctcac attcgtgttg ctaacagaag tattgctccg acagccaagg tttattccgt
     2821 ccgcatcaac ttaattggcg atattttcac aaaaaatcgc ccccacgcgg atccgcgcag
     2881 gggcaagcac ctggtaagaa tggactaggg tcattctacc ctgatcatct gccttctcaa
     2941 cttggtaaga atcctcttct ccagcctaga tatgtatgat tgagaaatgc caaggagatc
     3001 agcaacctgt ttttgagtct tttccgatcc gtccgaaagc ccgaatctca actccattat
     3061 ccgcttctcc ctccccgaca agctcgagag cgcctcgcca aggagtctcc ggtccacttc
     3121 ctcctcgagg gcctgataaa ctagatcatc ttcagtgccg tagacatcgg aaagcaagag
     3181 ctcattcccg tcccaatcca cgttgagagg ttcatcgaac gagacttcgc tccgtatctt
     3241 gttggtcttg cgcagataca tcagtatctc attctctatg catcgcgatg cgtaagttgc
     3301 gagcttgatc ttcttctcgg ggtcaaatgt atttaccgct ttgatcagtc cgatcgtgcc
     3361 tatggaaaca agatcttcca cgccgactcc cgtgctttcg aatttcctcg ctatgtagac
     3421 caccaggcga agattgcgtt ccacaagttc acttctgacg cagatatctc cggtcttcag
     3481 cttttccatg agaaatgctt cttcatccag gctcagagga gggggaagca tttcgtgact
     3541 cccaatatag cggacttggg agtcctcaac aatcacgaac caaccaatgg ccttgattat
     3601 gaagagccga attgcccaca tgatgctctc taaccgcttt ctcatgaaca agcctcccag
     3661 gaccgctcaa cccgcggtga ggatctcggg gttgagcagc gcgcggtatt ttccgttaga
     3721 acacaagctg gcgttatgca cgcagataat ggccctatct gtcgaaatct ccttctttcc
     3781 gtctatcacc ctaacttcac tcggccgaaa cccaaccata agccctctgg tctgccccac
     3841 agaagagtaa ggcactgccc tgaaacgtgt tgaccatgga gactccgcaa ttgcggcagc
     3901 tgccgccgca aaatcgccgt cgctaatctc caacatagcc ttgattgtat gaggcaggat
     3961 aggctcaata gcggagtatt ccacgattat tgccggaacg cccgatattg ggtctctcag
     4021 aagattcccg gtgtctataa gagctgttag cgagactgaa taccggtcga accttatcga
     4081 gataggcacg taaaacaagc cctcccgcat tccgcgatgg actgcccccc atcccgcttc
     4141 ggccactaac atcaccgtgg caacggatgc aatgaaagac aacggtctgc tgccgccagc
     4201 agcggaataa gccgccgtag ccgcaccacc tgccataacg gtgaggaggt atcggtagaa
     4261 gagggcgacg atgagctcgc gcgcgtccat tccaggaaag gcaatgacca atgatgcgcc
     4321 agcggccaag agtgcaacgg tcagtgacat gggcgatgac aggattatga tcccgagatc
     4381 ggagaga
//
LOCUS       DAOSAI010000203         4372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12618_length_4372_cov_3.846189, whole genome shotgun sequence.
ACCESSION   DAOSAI010000203 DAOSAI010000000
VERSION     DAOSAI010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12618_length_4372_cov_3.846189"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PLM83_07310"
     CDS             <1..1057
                     /locus_tag="PLM83_07310"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOK71247.1"
                     /translation="SKQQLVEIAKAFSAGARLIIMDEPTSSLPESEVQHLFDVVRTLN
                     RKGVSFIYISHRLEEVLAITDSMTVLRDGRLVGSCKTAETSYDDIVRMMVGRELTERF
                     PKREPSIGETVLEVEGLSDAGRFRDISFQVRAGEILGISGLMGSGRTELAQAIFGVVP
                     PEEGSIRLNGKELNVRCAEDGIEVGIYLVPEDRKRHGLVQTMSVRSNTVLSILSRLSG
                     AFGVVDKNKEEQVVTKAVRDFNVKTASIDHNVSSLSGGNQQKVVFAKAVCSGPKVLIL
                     DEPTRGVDVGAKAEIYKLVQDFASSGGAVVFISSEMPEILGMCDRILVMSEGRIVGEF
                     AGSEATQEEILKCALAK"
     gene            1078..2034
                     /gene="rbsC"
                     /locus_tag="PLM83_07315"
     CDS             1078..2034
                     /gene="rbsC"
                     /locus_tag="PLM83_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007440333.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HOK71248.1"
                     /translation="MMRRGVMQKYRILFVFVGICILLSVLTPVFLSVNNIINVIRQVS
                     INGILAAGMTMVIISGGIDLSVGSVAAICGAIVAGTQLKLGLVPAMLLSVATGALLGL
                     INGLVITKGKVAPFVATLGMTTVARGLTLIYTGGRPIYNLTDAFRVLGAGYVGPIPVP
                     VLILALVIAVVHFVMSSTVFGREVYALGGNEEAARYSGINVERRRMLVYISMGLLSAV
                     TGIVLTSRLGSADPTAGVGFETDAIAAVVIGGTSMSGGEGSVIGSLIGALIIGVMNNG
                     LNLLNVSPYYQQIFKGLIVVLAVLMDSASRRKRQAAALKVGR"
     gene            2144..3082
                     /locus_tag="PLM83_07320"
     CDS             2144..3082
                     /locus_tag="PLM83_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOK71249.1"
                     /translation="MRFSTKRTTQVVIGIIMVALCMVMNVGGAAVAAAAKPRTVAVAF
                     CTLNIPFFVEMENGIRAAAAKNNVKLIVTDAQDSAAKQLSQVEDLLVQKPDLLLITPV
                     DSDVLVPAVEAAYKAKVPVITIDRSVNSDKVVSFIGVDDVEGGRMAGRYVVEKLKGKG
                     NVVMLEGVMGSSAQRDRAQGFEEVIGKNKGIKMLAKQTANFNRGEAMTVMENWLQSFR
                     TIDAVFAQNDEMALGAIEAIRAAGRQNEIIVVGFDADPGAKQAVKEGKMAATIEGSPY
                     KLGVMGIQAAVDCLAGKKLPKQILIPLDLITKDTFK"
     gene            3514..>4372
                     /locus_tag="PLM83_07325"
     CDS             3514..>4372
                     /locus_tag="PLM83_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HOK71250.1"
                     /translation="MEKTKVIIMGAAGRDFHNFNVYFRDNERYEVVAFTATQIPDIAG
                     RMYPAELAGKLYPNGIPIYPEDQLEELIVKHDIDQAIFAYSDLSHVDIMHKASAVMAA
                     GADFRLMGPNTTMLKSRVPIVAIVAVRTGVGKSQTTRYVSQALIDAGLKVVAIRHPMP
                     YGDLAKQAVQRFATYEDLDIHECTIEEREEYEPHIDRGVIVYAGVDYGRILEEAEKEA
                     DVILWDGGNNDFSFYKPDLTITLVDPHRAGHEVLYHPGETNVRLADVVIINKVDTAKP
                     EDVETVRKNV"
BASE COUNT         1056 a         1020 c         1283 g         1013 t
ORIGIN      
        1 ttccaaacaa cagcttgtgg agatagccaa agctttctcc gctggagcgc gtcttattat
       61 catggatgaa cccacatcgt ccctgcctga aagtgaagtt cagcatctgt ttgatgttgt
      121 cagaacccta aaccgcaagg gggtcagctt catctacatc tcccaccgcc tcgaagaagt
      181 attggccatt accgattcca tgactgtgct gcgcgacggt agattggtcg gatcgtgcaa
      241 gactgccgaa acatcctatg acgacatcgt caggatgatg gtggggcgag agcttacaga
      301 acgctttcct aagagagaac cgagcatagg tgagactgtg ctcgaagtgg aaggcctttc
      361 tgatgctgga cgttttcgag acatatcatt ccaggtgcgc gccggagaaa tcttgggtat
      421 ttcaggattg atgggctcgg gccgcacaga actggcacag gcaatattcg gcgtagtccc
      481 tcctgaagag ggaagcattc gtctcaatgg caaggaactc aatgttcgct gtgctgaaga
      541 tggcatagag gtcgggatat acctcgtgcc ggaagacaga aaacgtcatg gtctagtgca
      601 gacgatgtct gtcaggtcca atactgttct ctcgattctg agcaggctgt cgggggcgtt
      661 tggggtagta gacaaaaaca aggaagaaca ggttgtaacg aaggcagttc gagactttaa
      721 cgtgaagaca gcttcgatag accataacgt gtcgagcctg agcggaggca atcagcagaa
      781 ggttgtattt gctaaggctg tgtgcagcgg accgaaggta ctcatactcg atgaacccac
      841 gcgtggtgtt gatgtgggtg caaaggctga gatatacaag ttagttcagg atttcgcttc
      901 ttcgggaggg gccgtggtgt tcatatcgtc ggagatgcct gagattcttg gcatgtgcga
      961 cagaatactt gtgatgtctg agggtcgaat agtaggtgaa tttgcgggat ccgaagcaac
     1021 gcaagaagag attctcaaat gcgctctcgc gaagtgacga cagaatacgg ggtgtaaatg
     1081 atgcgacgcg gagtaatgca gaaataccgg attctattcg tgtttgtagg gatatgcatc
     1141 ctgcttagtg tgctgacccc tgtcttcctt tcagtgaaca acataataaa tgtgatcagg
     1201 caggtatcaa taaacgggat actggcggca gggatgacaa tggtgatcat ctcaggaggc
     1261 attgaccttt ctgtcggatc tgtggctgcc atttgcgggg ctattgttgc gggaacgcag
     1321 cttaagctgg ggcttgtacc cgcaatgctg cttagtgttg ctacaggcgc attgctcggc
     1381 ctgattaacg ggcttgtgat taccaaaggc aaggtggcgc cgttcgttgc cacactggga
     1441 atgacgacgg tcgccagagg gcttaccctc atctataccg gaggacgtcc aatatacaac
     1501 ctcacagatg ccttcagagt gctaggagca ggttatgttg gcccgatacc agttcccgtt
     1561 ctcatactgg cactcgtaat tgcagtagtg catttcgtga tgtcgtctac ggttttcgga
     1621 cgtgaagtct atgcgctcgg gggcaatgag gaagcggctc gatactcagg aatcaacgta
     1681 gaacgcaggc gtatgctggt ctatatctcc atgggcttgc tgtcagcggt cacaggaata
     1741 gtccttacat cccgcttggg ctctgctgat cccacggccg gagtcggttt tgaaactgac
     1801 gccatcgcgg ctgtcgttat aggtggaaca agtatgtcgg gaggagaggg ctccgttatc
     1861 ggatctctca ttggagccct gatcatcggc gtcatgaaca acggattgaa cctcttgaat
     1921 gtgtcgccat actatcagca gatattcaaa ggcctcatag tagtccttgc ggttctcatg
     1981 gattcggctt cgagacgtaa acgtcaggcg gctgctttga aggttggcag gtagtgttag
     2041 aatgggaggg atgaagagct cagattaaac cataatgtag agtcgattcc acaggctgtt
     2101 ttgtctagtc actcaccaat caagttttgg aggtgccaac gagatgaggt tttcaacgaa
     2161 gcgtacaaca caggtcgtca tcggcattat catggtggcg ttgtgcatgg ttatgaacgt
     2221 aggcggcgca gcggtagcag ccgcggccaa acctcgaacg gttgcagtag cgttctgcac
     2281 cctgaacata ccgttcttcg tggaaatgga gaatggtatt cgcgcggcag cggcgaagaa
     2341 caacgtgaag ctcatcgtca ctgatgcgca ggatagtgcg gcaaagcagc tttcgcaggt
     2401 ggaggacctg ctcgttcaga agccggatct gctcctgatt acccctgttg attcagatgt
     2461 cctggttcct gcagttgagg ctgcatacaa agccaaggtg cctgtgatca caatcgacag
     2521 aagcgtcaac tcggacaagg tagtctcgtt cataggcgta gatgatgttg agggagggcg
     2581 catggcaggc cgctacgtag tggagaagct caaaggcaag ggcaacgtgg taatgctcga
     2641 gggcgtcatg ggcagctctg cccagcgcga ccgcgcgcaa ggctttgagg aggtcattgg
     2701 aaagaacaag gggatcaaga tgctcgccaa gcagaccgcc aacttcaata ggggcgaggc
     2761 catgaccgtg atggagaact ggctgcagtc gttccgaacg attgatgcag tgtttgccca
     2821 gaacgatgag atggctttgg gcgccatcga ggcaatacgg gccgcaggtc ggcagaatga
     2881 aattatcgtc gttggattcg acgctgatcc cggtgccaag caggccgtca aagaaggcaa
     2941 gatggcggcc actattgaag gctcaccata caaacttggc gtaatgggca ttcaggccgc
     3001 agtggattgc cttgccggca agaagcttcc aaagcaaata ttgattccgc ttgacctgat
     3061 tacgaaggat acgttcaaat agctgtaact gcatgttcat aggaacctag attagtcagt
     3121 gccctggggc tgtgcttgat gtgattagca cagccccagg gcgcatttct ttgtggcaga
     3181 acaattgact cgcatttccc ataaagcgtg aactggttcg atatcgccga agttgagatg
     3241 cttgtcgatt tccggcggca tatatgacaa tcattgaagg agttgatgcc ggagaagtcg
     3301 aatactcatg ggacgaatat gcaacgttgc ataaactatc tgcggcgtga tttctggcgg
     3361 ccatgtaggt tcctgattcg ccattgtttc gtatccatga ccccaattgc cccctagatg
     3421 ggggtgtgct gacgacttgg tgggcaatat actgaggaag cagtctttcc cacgcaggtg
     3481 ggatgcgcct tgaaaggacg ggatgtgtaa gacatggaga agacaaaagt gatcatcatg
     3541 ggcgctgcgg gacgcgattt ccacaacttc aatgtctact tccgcgacaa cgagagatat
     3601 gaagttgtgg ctttcacggc cacgcagatt ccggacattg ccggcagaat gtaccctgca
     3661 gagcttgcag gaaagctgta tcccaatgga attcccatct accccgagga ccagctcgaa
     3721 gagttgattg taaagcacga catcgatcag gcgatcttcg cctacagcga tctctcgcat
     3781 gtcgacatta tgcacaaggc ttctgctgtg atggcggcgg gagcggattt ccggctgatg
     3841 gggccgaaca ctaccatgct caagtcgaga gttccgattg tcgccattgt tgcggttcgc
     3901 accggggtcg gtaagagcca gacgacacgt tacgtgagcc aggcgctgat cgatgcaggc
     3961 cttaaagtgg tggcgatcag gcaccccatg ccctatggcg accttgccaa acaggcagtc
     4021 cagcgtttcg ccacttatga ggatctcgac atccatgagt gcaccattga ggagcgcgag
     4081 gaatacgagc cgcatatcga ccgaggcgtg atcgtgtatg cgggcgtcga ctacggcagg
     4141 atcttggagg aggccgaaaa ggaggctgac gtcatccttt gggacggtgg aaacaacgac
     4201 ttctccttct acaagcctga cctcaccatt accttggtcg acccacaccg ggcggggcac
     4261 gaggttctgt accatcctgg cgagaccaat gtccgccttg ctgatgtggt gataatcaac
     4321 aaggttgaca ctgccaagcc ggaagacgtc gaaacggtcc gtaagaacgt gc
//
LOCUS       DAOSAI010000204         4337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12741_length_4337_cov_4.164176, whole genome shotgun sequence.
ACCESSION   DAOSAI010000204 DAOSAI010000000
VERSION     DAOSAI010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12741_length_4337_cov_4.164176"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLM83_07330"
     CDS             complement(<1..717)
                     /locus_tag="PLM83_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOK71251.1"
                     /translation="MSDLLSPLCVKNMSLKNRIVMPPMASNTATLEGMATEATFSYYE
                     ERADRGVGLIIVEHTYVLKSGKYRERQLGIDCDDHVGPLSELTRRVHACGAAIGIQIT
                     HAGAATRSSTIGQRPLGPSVVPLSPEHEVPCEIERSFIPHLVKAFVQGAKRAVAAGFD
                     MVELHGAHGYLLNQFLSPITNIRSDEYGGSREGRLRFPLEVVTAVCSAVGPDAAIFYR
                     LGADDGVPGGLTIDDTCWAAQ"
     gene            complement(1088..1936)
                     /locus_tag="PLM83_07335"
     CDS             complement(1088..1936)
                     /locus_tag="PLM83_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOK71252.1"
                     /translation="MRITILDIFWLLIIVWSFVPLLSRQTRDSHRYRVIRNFEKRRRS
                     RVITLIHRQESTNLFGMTFGRHIDIEDSEQVLRAIRLTDPNVPIDIVLHTPGGLVLAT
                     EQIAHAIKNHPAKVTVFVPHYAMSGGTLIALAADEIVMDPNAVLGPVDPQIGSYPAAS
                     ILSAVARKPVAELDDQTLILADVSEKALKQVHGLIMKILADKMPPEKAAEITDQLSTG
                     RWTHDYPITCEELKQMGLSVRTDMPREIYDLMELYPQAASRKRPSVGYIPLPYGVRGE
                     QPKRRD"
     gene            complement(2369..3445)
                     /locus_tag="PLM83_07340"
     CDS             complement(2369..3445)
                     /locus_tag="PLM83_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOK71253.1"
                     /translation="MHYTVQSGDTLWLIAQRFRHDYRAIAQANSIEEPYDVRPGQVLH
                     IPRKHRLIGFVPDYAVAEGIADALASPEPFNEIIYTFIRIQPDGTVRAAALDRALQIL
                     RARRTRVLAGVTNQSDATYSREAVTATLLADDSRSHLAASIVEAVTTCCLDGVCIDFQ
                     QIDADHWAMLPKLACEIRSRFTRLSPYYTIAIVFPHDWRDHGLDLACMAQYADLMIME
                     GYEKGADSVEGPPTPVSWLEENVKAAIDKIGTGKLTVALGIYGLDWTDDEGAAYLRVE
                     AAERISRETGALIKRDQHDGTSGFVYHDASGRLHRVWYEDLMSLAVKLDTLSRLGINK
                     IAIWRLGAIPYDSLSLVAQAGMWS"
     gene            complement(3552..4094)
                     /locus_tag="PLM83_07345"
     CDS             complement(3552..4094)
                     /locus_tag="PLM83_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOK71254.1"
                     /translation="MSGKPVNWITRTAILLAMTLAFQVVFKLPQAFTGPVVNMFLFLS
                     VILVGMWSGAVIGLATPLVAWMVGIMAAGPLVPVIQLGNASLCIIFGLIDETGKGKSL
                     SIRILGIAVASVIKILFISLSAKHLLSLPEAAAKAMGSPQLFTALAGGALAVLVSEAL
                     LRTGAVTDRYGTTKKTTKSS"
     gene            complement(4116..>4337)
                     /locus_tag="PLM83_07350"
     CDS             complement(4116..>4337)
                     /locus_tag="PLM83_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOK71255.1"
                     /translation="AGLPMALGEFVGAAIGNISNGMLAVSLAFAGGAMVFITADELIP
                     DAQEFADGHSGTFGIVIGILAGMLLIYGY"
BASE COUNT          973 a         1140 c         1186 g         1038 t
ORIGIN      
        1 ttgtgcggcc caacatgtgt catcgattgt gagcccacca ggaacgccat cgtcagcccc
       61 cagcctgtag aatatggctg cgtcaggtcc gactgccgaa cagaccgccg tcacgacttc
      121 gagaggaaaa cggagacgtc cctctcgact ccccccatac tcatccgatc gaatgttggt
      181 gatgggagaa agaaactggt ttagtaggta gccatgtgcc ccatggagtt cgaccatgtc
      241 gaatccggcg gcaactgcgc gtttggcgcc ttgcacaaat gctttcacaa gatgcggtat
      301 gaagctgcgc tcgatttcac atgggacctc gtgttcgggc gaaagcggga cgaccgaagg
      361 acccaaaggc ctttgtccta ttgtgctgct gcgggtggcg gcgccggcat gggtgatctg
      421 aatgccaatt gcggcgccgc aggcgtgaac gcgccgagtt agctcgctga gggggcctac
      481 atggtcatca cagtctatcc cgagctgcct ttctcggtat ttgcccgact tgagcacgta
      541 cgtatgctct acgatgatca gtcctacccc gcgatccgcg cgctcttcat agtagctgaa
      601 cgtagcttca gttgccatgc cttcaagagt tgcagtgttg cttgccattg gcggcatgac
      661 tatgcggttt ttcaagctca tattctttac acaaagcggc gacagaagat ctgacaatgt
      721 tagtggagcc tcccgtattg atattgttga tgctgccttc acgcgacgaa ttcgattgtc
      781 ttaacgtaca cgagctgggc aagccttcgc cccgaaatcc gaagctaagc cgaggcttgc
      841 ttcggatttg cgtcattgtt agcccggtca tccgtgcgtc agtcgaaaag aacctcacca
      901 ggaattactc tgctgccaat tagccatcgg attcgatccg aaatctaacg gtaggagagt
      961 agaacatgag tcggcccaac caagctttga atgttgtcca caagccagca tccagccgca
     1021 gaggctctta aggcgtacag acgggctcag ctgagccgag gtcatagcat agtctaagga
     1081 cgcgttgtca gtcgcgtcgc ttaggctgtt caccgcggac accgtaggga agcggtatat
     1141 accccactga tggccttttc cgggaagcag cttgcgggta aagctccatg agatcgtata
     1201 tctccctagg catgtctgtg cgtaccgaaa ggcccatctg tttgagctct tcacatgtaa
     1261 ttggatagtc atgagtccag cgcccagtag aaagctgatc cgtaatttct gcagcctttt
     1321 cgggcggcat cttgtccgcc aagatcttca taatgaggcc atggacttgc ttcaatgctt
     1381 tctcggatac atccgcgagt attagggttt ggtcatctaa ctcggcaacc ggctttctgg
     1441 ccacagcgga aagtatcgat gctgcgggat atgagcctat ttgagggtca actgggccaa
     1501 gcacggcatt gggatccata actatttcgt cggcagccag ggcgataagt gtgccgcctg
     1561 acatggcgta gtgagggacg aatactgtaa ctttggccgg atgattcttt atggcatggg
     1621 caatctgctc cgtggccagg acgagtcctc ccggagtatg gaggacaata tcgatgggaa
     1681 cgttcgggtc agtcagcctt atcgcccgca gaacctgttc cgagtcctca atatcgatat
     1741 ggcggccgaa ggtcatcccg aataggtttg tcgattcttg cctgtgtatc agcgtgatga
     1801 ctcgggatcg tcttctcttc tcgaaattcc gtatgacacg atatctatga gaatctcgtg
     1861 tctgtcgaga aagcaatggc acgaaactcc acacgatgat aagaagccag aagatgtcca
     1921 gaatagtgat gcgcatgtgc tcccctcccg atcatttgtt attctatgtt tccgcatatc
     1981 gattatagcc ttattatcct tccaattcca taaggctagt agtaatctgc tgatgatggt
     2041 tgtgcgcatg ggtgttttcc tagggctagg cggaagagcg gagggatgct catgatggca
     2101 cttcgccaga gatgacacat ggggttagct agagtgaaga tttggcaatg gccgtggcga
     2161 aacagggaag gccccgaact gctggttttc gggcgaacca aagagtgtac tggccacttg
     2221 catgttgcgt agccaaggcc ctgcgcagtg cggcaggaca tcgagcttgt ccatgttctt
     2281 cactcagttg ttgctccgtc ggcaagttga gggcgcgatc ggattgcgtc gcgttctcca
     2341 tgaaaatcgg gaccggcatg ggctatcgct aactccacat gccggcctgt gctacgaggc
     2401 tgagagaatc ataggggatg gcccccagac gccatatcgc tattttgtta atgcctagcc
     2461 tggaaagggt atccaatttg acagccaaag acatgagatc ttcataccaa acccgatgca
     2521 acctgcctga agcgtcgtgg tagacaaatc ccgaagtccc atcatgctgg tcgcgcttga
     2581 tcaaggcgcc agtttcccgt gatatccgct ccgctgcttc tactctcaga taagctgcgc
     2641 cctcgtcatc ggtccaatcg agtccgtaga tgccaagcgc gacagtgagt ttgccggtcc
     2701 cgatcttgtc tatagcggcc ttcacattct cttccagcca tgacacggga gtcggtgggc
     2761 cttcgacgga gtccgcccct ttctcgtagc cttccattat cattaagtcg gcatattgag
     2821 ccatgcaggc taagtcaagc ccatggtcgc gccaatcgtg cgggaatact attgcaatgg
     2881 tgtagtaggg gctgagtcta gtgaacctgg aacggatttc gcatgctagc ttgggcagca
     2941 tggcccaatg atctgcatcg atttgctgga agtcgatgca aaccccgtcg aggcaacagg
     3001 tggtgacagc ttccacaatc gatgctgcaa gatgtgaacg gctatcgtct gccagcaggg
     3061 ttgcggtaac cgcctcccta gaataggtgg catccgactg gttggtgact ccggccaaca
     3121 cgcgtgtgcg ccttgcccga aggatttgga gtgcgcgatc cagggcggcc gccctcactg
     3181 tcccatctgg ttgtatgcgg atgaaagtgt atatgatttc attgaaaggt tccggagacg
     3241 cgagggcgtc tgcgatccct tccgcaaccg cgtaatcggg cacaaacccg atcagcctgt
     3301 gctttcgcgg aatgtgaagc acttgcccgg gacgcacgtc gtacggctct tctatggagt
     3361 tagcttgtgc tatcgctcga tagtcatggc ggaatctctg cgctatgagc catagagtat
     3421 ctccgctttg gactgtataa tgcattcatg ccgcctcctg cgagcggtga taacctgaac
     3481 actacaagct atgcaaaagg tcataaaaaa gttcccgctc atggagacgg catacttagt
     3541 atatttgcgc gctatgagga tttggttgtt ttctttgtcg tcccgtatct atccgtgact
     3601 gctccagttc tgagcagcgc ctcagataca agcactgcga gggcgcctcc tgccagcgcc
     3661 gtgaagagct gcggggagcc catggctttc gcggctgctt caggcaatga cagaaggtgt
     3721 ttggcgctta atgagatgaa gagaatcttg atcacagaag cgaccgcgat acctagaatg
     3781 cggatggaca gactcttgcc tttgcctgtt tcgtctatga ggccgaagat gatgcacaaa
     3841 gatgcatttc ctagctggat aacggggacc aggggccctg ccgccattat ccctaccatc
     3901 catgcgacaa gcggcgttgc aaggcctata acggctccgc tccacatgcc aacgaggatg
     3961 actgagagga atagaaacat gttgactacg ggtccggtaa atgcttgagg caacttgaag
     4021 accacctgaa acgcgagggt catagccaac agaattgctg ttcgagttat ccagttgact
     4081 ggctttcctg acatcagcac cacgctccta tgcctttagt atccgtaaat gagcagcatg
     4141 cctgcgagta ttccgatcac tattccgaag gttccagagt gtccatcagc gaattcctga
     4201 gcgtcaggta tgagctcgtc agcggtgatg aacaccatgg cgcctccagc aaaagccaga
     4261 gagacggcga gcatgccatt ggagatgttt ccgatagcag cgcctacaaa ttcccctaac
     4321 gccattggca gacctgc
//
LOCUS       DAOSAI010000205         4337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12742_length_4337_cov_3.471742, whole genome shotgun sequence.
ACCESSION   DAOSAI010000205 DAOSAI010000000
VERSION     DAOSAI010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12742_length_4337_cov_3.471742"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..904)
                     /locus_tag="PLM83_07355"
     CDS             complement(5..904)
                     /locus_tag="PLM83_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOK71256.1"
                     /translation="MTTSALILVLVSAVIHAGWNLATKKAEGGAAYVFLMAAGSALCW
                     FPLAAWLLMTGNTTTPGASPFSVVVAISVSGAIHTAYFLSLQAGYGCGDLSLVYPLAR
                     GTGPLLSTLAAMLLYGERPSGLALAGALLIGVGAFFLAGAEEPQATPSDDGSSKAIVY
                     GLLTGVLIAGYTMWDKYALDVVGVKPAFLSSAANCVRAIGMAPFFLLSKKRRAEVSTA
                     MRTQWKPALIVSISSPLSYLLVLTALATSPVSYVAPAREISILLGTLAGTRMLEEGDP
                     SSRKKRLWASVAMAAGVVALAIG"
     gene            1134..1871
                     /locus_tag="PLM83_07360"
     CDS             1134..1871
                     /locus_tag="PLM83_07360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOK71257.1"
                     /translation="MLCHRLIALDLDGTVLTDDKRIADSTKLWVGKAADAGVIVTFAT
                     GRGRQDALSFVRELDLDSPMVFLNGAEIWGGPGQLLERHFMDTEDIYRLHQLTAETGC
                     CFWGYTVETLIQNDWSNDMLHQEWMKYGMYCDDVDLMAQLRDYVDSWGTVEVTCTSDA
                     NMEVTARGITKARGVRRICEQIGIEMEAVMAVGDGLNDLDLIEGAGLGVAMGNAAEEL
                     KRAADFVTESNEDDGVVLAIRRCIFGM"
     gene            2279..3859
                     /locus_tag="PLM83_07365"
     CDS             2279..3859
                     /locus_tag="PLM83_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK71258.1"
                     /translation="MSKRTVLAIVAALLLVSASTVVLGAGPTKGGTLKIAASSIQQLD
                     PYKTAANDETNLCGLVFDPLVVIGKDSFEPKPHLAQSWEMPDENTWVFHIRKGVYFQE
                     GNEVFKKGAKREVTADDVVYSINRFINVSTAFTLGPIKSVKALDRYTVEIKTPEPSPF
                     LVSDPNRLPSVGIVPREAIEKLGEDGFARKPIGSGPFQLKSFSPDKGAVLERNPRYWL
                     PVYLDKVEFVVIPDPTVQVMAVTSGQVDIITYLVNMDAMPTISKNRKLAAVRGRGGSY
                     RGLGFNVTVSPFDEFAVRDAISKAMNIDAAFNAVVAPHGQRAYGQCPPWVAHGYDPSL
                     KSLWKYDPKEAVAILNKAGFRDTNGDGILDRNGQPLKVEIKTVPGSQVRVLTILATQL
                     RQIGIDASVVQQDTAVWVSDIMGGKSGVFFDFSFAGTTGLHSLFHSEAIGKTNGHFYA
                     DSTVDSLLDQALRTTDMKKLSSLWKQAQRKIMEDRASIPLYFEWGYSVVNKKVNDYVS
                     PWGGLHLVTLENNVWISK"
     gene            3993..>4337
                     /locus_tag="PLM83_07370"
     CDS             3993..>4337
                     /locus_tag="PLM83_07370"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71259.1"
                     /translation="MTRYIVRRLLATVPVIIGITFIVFAMVSFAPGDPIELMLGSETV
                     NPEYAERIRIELGLDRPWYEQYLRYMSGLLRGDMGRSIVYNRPVAGQVKERFANTAIL
                     AVSSAIFALLISI"
BASE COUNT         1041 a         1077 c         1227 g          992 t
ORIGIN      
        1 gctctcatcc aatggccaag gctaccacgc ctgccgccat tgcaacagat gcccacaagc
       61 gtttttttct tgagctgggg tctccttcct cgagcatccg agtcccggca agtgtgccca
      121 ggaggatgct gatttcccgt gccggggcga cgtagcttac aggcgatgtt gccagggccg
      181 tgaggaccaa aagatacgat aggggtgatg atatggagac tatgagggca ggtttccatt
      241 gagtgcgcat ggcggtgctc acttcagctc gacgcttctt cgataatagg aaaaacggcg
      301 ccattcctat ggcacgcaca caattggcag ccgacgacag aaacgccggt ttgacgccga
      361 ccacatcaag agcgtacttg tcccacatag tgtaacctgc gatcaaaacc ccggtgagga
      421 gcccgtacac tattgctttg ctgctgccgt catcagaagg cgtagcctgc ggctcctcgg
      481 cgcctgcaag aaagaatgcg cctactccta tgagcaatgc gcctgccaac gctaatcctg
      541 atgggcgctc tccatacaaa agcatcgccg ccaacgtgga taacagcggg cctgtcccgc
      601 gcgccagcgg atagacgagg gatagatccc cacatccgta tcccgcttgt agagacagga
      661 aataggctgt atggattgct ccactgacac tgattgcgac gactacagag aaaggggatg
      721 ctcctggcgt ggtcgtgttc ccggtcatca acagccacgc tgccaatgga aaccaacaca
      781 atgccgaccc ggcagccatc agaaacacgt atgccgcgcc gccttcagct ttcttagtcg
      841 ccagattcca gcctgcgtgg atcacagccg aaacgagtac caagataagt gcggaagttg
      901 tcactgcttc acctccttcc gacgaatatt ctcttttacc gattcaatcc ctgcttgcgt
      961 caggcggatg aagtggtgcc gggggcatgg cagcactttg cctctaaagt cggaagtgat
     1021 atagtctaca tgcagcgcag ggcctggtta tgatggagtt gggaagtgtc tgaggatggg
     1081 tttcccggat gcactgcgtt ctgcatgaga ctgcaaggag gatgagttgg aatatgctct
     1141 gccacagact tatagccctc gatctcgatg gtacggtgct cacagacgac aaaagaattg
     1201 cggacagtac gaagttatgg gtcggaaaag ccgcagatgc cggggtcatc gtcacgtttg
     1261 ccacggggag gggaaggcaa gacgcgcttt cattcgtgcg cgagctcgat ctcgattcgc
     1321 cgatggtgtt cttgaatggg gctgagattt ggggtggacc tggacagctg ttggaaaggc
     1381 acttcatgga tactgaggac atttaccggt tacaccaact gacggcggag actggatgct
     1441 gcttttgggg atacaccgtg gagaccctaa tacagaatga ctggagtaac gacatgcttc
     1501 atcaagagtg gatgaagtac ggcatgtact gcgacgatgt cgacttaatg gcccaattac
     1561 gcgactacgt cgacagctgg gggacggtcg aggtaacctg cacgtctgat gcgaacatgg
     1621 aagtcactgc tcggggaatc acgaaagccc gaggagtcag gagaatatgc gaacagatag
     1681 gcatcgagat ggaggcggtc atggctgttg gagatggtct caacgatctg gacttgattg
     1741 aaggcgcagg attgggcgtg gcaatgggca atgccgcaga agagctgaag cgggctgccg
     1801 actttgttac tgaatccaac gaggatgacg gtgtggttct ggcgattcgc cgttgtatct
     1861 ttggcatgta attgctattg gcgaagggac aatggcgacc gaatatcccc cccgattggc
     1921 ttccaaattt cattcttgta ttgtttccca gacgacagtc tataaaactg atgatggcgc
     1981 ttgaaagcct ctaacctgag ggcctagcgg aagtgtatca cggagtgaca tcgctggccg
     2041 gcgacctatg gtgttcccta ggaagccgca aagagcgccc cagcattatc aagaaactct
     2101 atcgaggctt gtccagacac atacgatgtg cgttgggccg tgccccatta ttataagcat
     2161 tctttgtaca attattgtga ataattttct gtttgactta tctagctcgc acacgtataa
     2221 ttacagacta atatctatta acgatttatc attagatgca gcaagggggc aacaggacat
     2281 gtcgaaaagg acagttctag caatcgtggc agcacttcta ttggtgtctg catcaaccgt
     2341 ggttttggga gccggtccga ctaagggcgg cactctcaag atcgcggcgt catccatcca
     2401 acagcttgat ccatacaaga ctgccgccaa tgacgaaacc aatctgtgcg gcctcgtctt
     2461 cgatcccctc gtggttatcg gcaaagatag tttcgagccg aaaccccacc ttgcgcaaag
     2521 ttgggaaatg cctgatgaga acacttgggt tttccatatt cgcaaaggcg tgtacttcca
     2581 ggagggcaat gaggttttca aaaagggcgc caaacgtgaa gtgacggcgg acgacgtcgt
     2641 gtattccatt aatcgtttca tcaacgttag cactgctttc acccttggcc caatcaagag
     2701 tgtcaaggca ctcgatcgtt atactgtcga gatcaagacg cctgagccgt cgccgtttct
     2761 ggtcagcgat cccaaccgat tgccaagtgt gggcatagtg cctcgtgagg caatcgagaa
     2821 gcttggcgaa gatggctttg ccagaaagcc cataggctca gggccattcc agctcaagag
     2881 cttctcacct gacaaaggcg ctgttcttga gcggaatcct cgctactggt tgccggtata
     2941 tctcgataaa gttgaattcg tcgtcatacc tgatccgacg gttcaggtaa tggccgttac
     3001 ttcaggacaa gtggacataa tcacgtatct tgtgaacatg gacgccatgc ccacgatttc
     3061 gaagaaccgc aagctggctg ctgtgagagg tcgtggtggc tcatacaggg gtcttggttt
     3121 taacgtgact gtttcgcctt tcgatgaatt cgcggtgcgc gatgcgattt cgaaggccat
     3181 gaacattgat gccgcgttca acgcagtggt agcgccccat ggccagcggg catatgggca
     3241 gtgccctccc tgggttgccc atggctatga tccaagtctg aagagcctgt ggaaatatga
     3301 ccctaaggaa gccgtagcaa tactgaataa ggccggattc cgagacacaa atggcgatgg
     3361 aatacttgac cgaaatgggc agcccttgaa agtggagata aagactgtcc ccggttcgca
     3421 agtgagggtg ctcacaatcc tcgctacaca gctcaggcag atcgggatag atgcatcagt
     3481 agtgcagcag gatacggccg tttgggtgtc tgacatcatg ggcgggaagt ccggcgtgtt
     3541 cttcgacttc agtttcgcag gaaccactgg tctgcattcg ctcttccaca gtgaggcgat
     3601 tgggaagacg aatggccact tctatgccga ttccaccgta gactccctgc tcgatcaggc
     3661 gctaaggacc actgacatga agaagctgtc gagcctctgg aagcaggcgc aacgcaagat
     3721 catggaggac agggcctcga taccgctcta cttcgaatgg ggatactcgg ttgtaaacaa
     3781 gaaagtcaat gactacgtgt cgccgtgggg cggtctccac ctagtgacac tcgagaacaa
     3841 cgtctggatt tcaaagtagc gttcattcct gacagattga ttcgtacggg aagatgaacc
     3901 caggaagctt cgatggggag ggatacgttg cttggcgacg cagccttgaa atgtagcgcc
     3961 ctcccctgtg aagttagggg ggaaggtcag agttgaccag atatattgta cgtcgtcttt
     4021 tggcgacagt gccggttata atcggcatca ccttcatagt gtttgcgatg gtcagtttcg
     4081 ctcccggcga cccgatcgag cttatgctcg gatcggagac tgtaaatccg gaatacgccg
     4141 agcgaattcg gatagaactg ggcctcgaca ggccatggta cgagcagtat ttgcgatata
     4201 tgagtggatt gctccgtggc gatatgggaa gatcgatagt ctacaatcgt ccggtagcag
     4261 ggcaggtcaa agagaggttt gcaaacacgg ccattcttgc cgtatcgtcc gcgatatttg
     4321 cgctactgat ctcgatc
//
LOCUS       DAOSAI010000206         4323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12806_length_4323_cov_3.936973, whole genome shotgun sequence.
ACCESSION   DAOSAI010000206 DAOSAI010000000
VERSION     DAOSAI010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12806_length_4323_cov_3.936973"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLM83_07375"
     CDS             <1..613
                     /locus_tag="PLM83_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOK71260.1"
                     /translation="EFLTSLIPVIPRPWAPPIVRWMCACAAQAGVGPMAAVAGAISET
                     VGISLLSEDSLSKLWQREPDIGNPDGHRARISEVIVENGGDVFIASSRLRRVGIYAGT
                     SPLSGKIALEIAPGQTPCGICTSSGTVGHSRSFGRCDGAVIVAKDTALADAVATRVAN
                     VVKSREDIEEALDTASKIPGIVGAVIVIGDALGAWGDIKLAPA"
     gene            complement(1520..2077)
                     /locus_tag="PLM83_07380"
     CDS             complement(1520..2077)
                     /locus_tag="PLM83_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71261.1"
                     /translation="MDNGTYEEIGKKSLAAFSNLLDILSGARTSPALADPVGLERRIG
                     EQLATVVASLDLVRRDLAALASRTDRALLELLKRIEPQEGLAVTSFPTPSLPASQVSA
                     IGTAGPGTGGRAATPSKPRGKYERVPKEQTREKILEAARELAAAGRKITLAECARMAD
                     VAYYKAVYACKDSEELEELMSGNAG"
     gene            2383..4209
                     /locus_tag="PLM83_07385"
     CDS             2383..4209
                     /locus_tag="PLM83_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOK71262.1"
                     /translation="MKAEINLPSTAVVLSLDPRLEASIRVAVSLRREPFPSDADMRII
                     TCSTRAGAAEALARYVGQPVLSIVLVDVSPGGGFTLDDVTLMARQFPTIPVVAVVPRN
                     KEDLEYEALRAGACDYLIKGVITPSIFARMTRSAENYWSAKSLAFEGLEKAKTLAQAI
                     PIALMLVDDRGIVIGGWNPAAEEVFGVARRDALGRPMPAVKWTGKEETAAILARAVAG
                     DSISGIEVQCRRADGELIDAIAFFTALYEGDELKGILALFEDITQRKRAENALRDSEV
                     RFRGVFEGSQLGMAIADTNMRIIHANPALGELFGCLPEDLAGVMAEELIHPDDEADIA
                     SRVSRLLRDRISFFTAESRYVRMDGTVFVGRLLATLFSVSLEGPHYVLFALEDITLRK
                     IAQDQLEASLVKLAQTTEGAIHAMSRVVEMRDPYTAGHQERVACLARHIAMELGYPKD
                     AVDGIYFGSLVHDIGKIVVPGEILTKPGKLSPIEFSLIKAHPEEGFKVVNSIEFPWPI
                     ANMVLQHHERLDGSGYPAGLAGDAICEGAKIIAVADVVEAMSSHRPYRPSLGMDAALD
                     EIVSKSGILYDPEVVQACVNVIAVHGDRALMGTVSDLNGEMP"
BASE COUNT          877 a         1095 c         1352 g          999 t
ORIGIN      
        1 cgagtttctc actagcctca tcccggtgat tccgcggcca tgggcgccgc ctattgtcag
       61 atggatgtgc gcctgtgctg cgcaggcagg ggtggggccc atggccgccg tggccggcgc
      121 cataagcgag acggttggga tcagcctcct gtcggaggat agcctttcta agctgtggca
      181 gcgcgagcct gacattggga atcctgatgg gcatcgagcg aggataagtg aagtgatagt
      241 ggagaatggg ggagacgtgt tcatcgcctc cagccgtttg cgcagggttg gaatctacgc
      301 gggcacgtcc cctttgagtg gcaagattgc cctggagatt gctccgggcc agaccccttg
      361 cggcatctgt acgtcttctg gaacggtcgg gcattcgcgc agtttcggta gatgcgacgg
      421 agccgtgatc gttgcgaaag acacagccct cgccgatgcc gttgccacta gggtcgcaaa
      481 tgtcgtgaaa tcgagggagg acattgaaga ggcgttggac acggcttcga agattcccgg
      541 catagtcggg gctgttatcg tcatcggcga tgccttgggg gcatggggcg atatcaagct
      601 ggcgccggcg tgaagaaaca tagcaaatcg caacatggct gacattgagc ccggttccat
      661 tttatctggg gcaacataga ataagccaga cctacctcag gaggtgaccg gacgtggccg
      721 acgtgactgc cagccagtgc ttctgccctt ggtggttctg gctttgggtt gcgatcatcg
      781 cattcatcgt gtgctgcatc tgccgtttcg tgctttgctg gtaaggcacg attttcctcc
      841 gcgcaagtaa gatcaagttc gcttcaggcg tcatagtatg gaccagacat gcatcaggag
      901 gtgaccggat gtggccgaag tgactgccag ccagtgctgt tttccttggt ggatttggtt
      961 ttgggtagtg gtaatcgcat tcatcgtgtg ctgcatttgc cattttatgc tttgctggta
     1021 agaaccagct cgtccccacg ctggcaaaac agctcatcgc ctatatcgag cttggacaac
     1081 ggcccacgcg ccatccgccc gccacggtgg gcggatggcg ttggtgatgc gtcccgccgc
     1141 acctgttaaa agaaaggacg gacccgttgc aaggactgat atccttcacc acacaaaaga
     1201 tgacaggaaa ggttggagat gagcttttat cagaaggcgc gcggcagggt cttccgcgaa
     1261 atggtcgtca catattggcg ccgtgaccgc gttgatgttg atagcgataa tcgcgtgctg
     1321 gattctgaag cccgagcgga aacccggacg tgtctataga gccgattgtg ggtgccgctt
     1381 tggctcctga tcgatgatga tcagcatttc cgcctcgcac aagaacagag cagcaggact
     1441 gcggcatcgg tggtctcttt ccctgcgcga tttagagcag ggaagggcgt cgtgccgcag
     1501 tcagctttgt ctccactcat cagccagcgt ttccgctcat cagctcctcg agttcctcag
     1561 agtctttgca ggcatacacg gccttgtaat aggccacatc cgccatgcgg gcgcattctg
     1621 caagggttat ctttcggccg gcagccgcaa gttctctggc ggcttcgagt atcttctctc
     1681 tcgtctgttc tttgggcact ctctcatact tccctcgcgg tttcgacggg gtcgcagcgc
     1741 gtcccccagt tcccggaccc gcggtgccga tggctgaaac ctgtgaggca gggaggctgg
     1801 gtgtgggaaa tgatgtgact gccaatcctt cctgaggttc gattctcttc agaagctcga
     1861 gaagagccct gtctgttctg ctggccaagg ccgcaaggtc gcggcggact aagtcgaggg
     1921 atgccacaac ggtggccagc tgctctccga ttcgcctttc cagacctacg ggatcggcaa
     1981 gggcaggcga agtccttgcg cccgacaaga tgtcgagtag atttgagaat gctgctagag
     2041 acttctttcc aatctcttcg taagtgccat tgtccacatt agagacctcc tcgtacgacg
     2101 gtgtttcttc tctactgcgc ttgagtgccg cttcttgcac aatagcggca taatagcgct
     2161 gtttcccacg agagcgcaat tgggttgcac ctcctgccat attattattt ccaaactcgt
     2221 tcataatgtg tcatatggat ggaagatcgt ctggatgacg cctgccccga accgtgttgg
     2281 ctcgggaagg gttgtctcaa agtgtttatt gcgcctacaa tatactagcc ggggagagtc
     2341 ggcgctattc atctttatct tcagaatacg gaggggtgaa gggtgaaagc ggagatcaat
     2401 ctgccttcga cagcggtggt tttgagcctt gatccacggc tcgaggcttc aatccgggtt
     2461 gctgtgtcat tgcgcagaga gccctttcct tcagacgcgg atatgagaat cataacttgc
     2521 agtactcggg caggggctgc ggaagccttg gcccgatatg tggggcagcc cgttctctcc
     2581 attgtccttg ttgacgtgtc gcctggaggc ggctttaccc tggatgacgt taccctcatg
     2641 gcgcggcagt ttcctacgat tccggtggtg gctgttgtgc ctcgcaacaa ggaggacttg
     2701 gagtatgagg cgcttcgcgc cggggcgtgc gactacttga taaaaggggt catcactccg
     2761 agcatattcg cgaggatgac aaggagcgca gaaaactact ggtctgcaaa gagcctggcc
     2821 tttgagggac tagaaaaggc caagaccttg gcgcaagcga ttcccatagc tctgatgctc
     2881 gttgatgatc ggggtatcgt gataggtggc tggaacccgg cggctgaaga ggtgtttggc
     2941 gttgcgagaa gggacgctct cggcagaccg atgcctgcgg tgaaatggac tggcaaggaa
     3001 gagactgcag ccattctggc gagggctgtt gccggcgact ccatatctgg aatcgaggtc
     3061 cagtgtcggc gcgcggacgg ggaattgata gatgcaattg ccttctttac cgcgctttac
     3121 gagggggacg agctcaaggg catactggct ctttttgagg atatcaccca acgaaagcgg
     3181 gcggagaacg cgctgcgcga cagcgaggtg cggttcaggg gagttttcga gggaagccag
     3241 cttgggatgg cgattgcgga cacaaatatg cggattatcc acgcaaaccc cgctcttggg
     3301 gagctgttcg gatgtctgcc ggaggacttg gccggggtca tggcagaaga actgatccac
     3361 ccggacgatg aagcggatat tgcgtcgcgt gtgagcaggc ttctgcggga caggataagc
     3421 ttttttacag cggagagccg gtatgtgagg atggacggca ccgtctttgt aggcaggttg
     3481 cttgcgactc tgttcagcgt cagcctggaa gggccgcact atgtcctttt tgctctggag
     3541 gacattacat tgcgcaagat agcccaagat cagctcgagg ccagcctggt caagctcgcg
     3601 cagactacgg agggagccat ccatgcaatg tcgcgggttg tggagatgcg cgacccttat
     3661 accgcgggcc atcaggaacg cgtggcttgc ctggcacggc acatcgcaat ggaactggga
     3721 taccccaagg acgccgtgga cgggatttac ttcggctcgc tggtgcatga cataggcaag
     3781 atcgtggttc cgggggagat actgactaaa ccgggcaaac tgagcccaat tgagttttcg
     3841 ttgatcaagg cccaccctga agagggtttt aaggtggtca attcgataga atttccctgg
     3901 cccattgcga acatggtcct tcagcatcac gagaggctgg acgggtcggg ttatccagcc
     3961 ggcctagccg gggacgcgat ctgcgagggg gcaaagataa ttgcagttgc cgacgtggtg
     4021 gaggcaatgt cgtcgcacag gccttacagg ccttctttgg gaatggatgc agcattggac
     4081 gagatagtga gcaaaagtgg tatactgtac gatcctgaag tggtccaggc ttgcgtaaat
     4141 gtaatcgcgg tccatggcga ccgcgctctc atgggaacgg tttcggattt gaatggagaa
     4201 atgccctgag ctgtcagggc atttctcatg cgatctatgc tagcttgaac ctggctgcaa
     4261 gctgctgtag ccttgctgcc atgtcggcaa gggaatggga agcggaggac atctgctgca
     4321 acg
//
LOCUS       DAOSAI010000207         4291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12933_length_4291_cov_3.822002, whole genome shotgun sequence.
ACCESSION   DAOSAI010000207 DAOSAI010000000
VERSION     DAOSAI010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12933_length_4291_cov_3.822002"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="PLM83_07390"
     CDS             complement(<1..1309)
                     /locus_tag="PLM83_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOK71263.1"
                     /translation="MSTPNGYLALVLHAHLPFVRHADGEHYLEEKWFYEALIETYIPL
                     YIVLSRLADEEVPYRITISVSPTLLSMFRDPLLQQRFSDYMNNLVELAEREVARTRWL
                     PAFHDTAQMYRDRFREARDVYWNRLGGDMAKGLQDLSDSGHVELITTAATHGYLPLME
                     VCPEAARAQILAGIDYYRQVFGIAPKGFWLPECGYSPGIDEYLREAGVRYFFIDSHGI
                     LNASPRPRYGVYAPVYCHSKVAAFGRDMESSKQVWSAQEGYPGDYDYREFYRDIGYEL
                     DWESIKPLMGNCPIRLQTGLKYYRVTGNTDRKEPYVRQWAIEKAATHAGNFVFNRERQ
                     VEYLASVMDRKPIVVAPYDAELFGHWWFEGPEFLDYVIRKIAYDQNTIALLTPSDYLE
                     LYPKNQVATPSMSSWGYMGYSEVWLADCNNWIYPHLHTMAGRMT"
     gene            complement(1313..2335)
                     /locus_tag="PLM83_07395"
     CDS             complement(1313..2335)
                     /locus_tag="PLM83_07395"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOK71264.1"
                     /translation="MQDSFDKVSPIDKKGAGTPSVSQHEQQMAEDARIELSPPEVSDQ
                     PLPHRRFPEITILETENLQMNPARRHKPTVLPGFYGATRIVLQVRDPFWMHAYWEIAE
                     DMAKNLRRQLGEELDRSQLTLRVHDVTGVTFDGHNSVRSFDKEVHPFANNWYINVEES
                     DRSYCVDLGLVTGASQFYLISRSNTVHTPRIGPSWIVDEEWLTIREIEEGYISHGLSP
                     SSPGISRHARAEELLHEIALGSGGVGAVGSPMGQPGEVHRAPRGFWLRADAELIIHGA
                     TEAGAKVEVCGMPMPVRPDGSFSVRLSLPFGDHPVDISATSDDGIMHRSVSWIVSRSN
                     RGNGRS"
     gene            2502..4100
                     /locus_tag="PLM83_07400"
     CDS             2502..4100
                     /locus_tag="PLM83_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOK71265.1"
                     /translation="MDHIGTFSLVLHSHIPYCRRSGVWPFGEEWVFEAVVETYIPILD
                     WLLNCHEKGVGPNVTLSFSPVLAEQLADAYMKSRIVEYMDARANAARADLMRHELVHD
                     DRLARLSRKYVEYYENAKKRFIDYYDGDLLGAIAKLAASGRVEVLATSASHAYLPLLP
                     QESRIRTQIRVGYEACQRHFGVLPKGAWLPECGYKPGAIERALEECGIEYFIVDTPAI
                     AGGGPRNLYGGVPEALRRNIGAGLPSMATLMPYRLRGSKMAVFGRDEVTAAQVWSRDV
                     GYPGDGNYLEFHKRDDSSGLRYWRVTNKLLDFGQKQLYDPEAALASARTHAQHFAGVV
                     EERLGGYAERTGRHGIIVAPYDMELFGHWWHEGVDWLSLAMDALCASNRVECGTLGSY
                     LKLHPPVDELELPECSWGHGGRHGVWFNSETEWMWQEIERATAIGRELEGVIGGLDGV
                     HKIMADQALRELMLLEASDWPFLVTTGQAGGYARDRFAQHLSDFEVLAQAAMARETDL
                     RSLEVLEASSSRDSVFTWIDWGSL"
BASE COUNT          934 a         1076 c         1209 g         1072 t
ORIGIN      
        1 ccgtcattct ccctgccatt gtgtgtaggt gcgggtatat ccagttgttg caatctgcca
       61 gccatacctc gctgtacccc atgtatcccc aagacgacat ggatggagta gcgacctggt
      121 ttttcggata gagctccagg tagtcggatg gggttaacag ggctatggtg ttttggtcgt
      181 atgcgatttt gcggatcacg taatcgagga actcaggacc ttcgaaccac caatgaccaa
      241 aaagctctgc atcgtaagga gccactacta tgggcttgcg gtccatcact gatgctaagt
      301 attctacttg acgctctctg ttgaagacga aattgcccgc gtgtgtagcg gctttttcaa
      361 ttgcccattg acgcacatat ggctcttttc gatctgtatt tcctgttacc cggtagtact
      421 tcaacccagt ttggagacga atagggcagt tccccatgag gggtttgatc gattcccaat
      481 caagctcata tccgatatct cgatagaact cccggtagtc gtaatccccg gggtaacctt
      541 cctgtgcgct ccaaacctgt ttggatgatt ccatgtcgcg accgaatgct gccacttttg
      601 aatggcagta cacgggggca tacactccat atctgggcct aggcgaagca ttgagtatgc
      661 cgtgggagtc gatgaagaaa tacctcactc cggcttctct gaggtattca tctatccctg
      721 gtgagtaacc gcactccggg agccagaaac ctttgggagc gattccaaat acttgcctgt
      781 agtagtcgat tcccgcaaga atctgcgcgc gggcagcttc gggacaaact tccatcagcg
      841 ggagatatcc atgtgtggct gcagtagtga tgagttcgac atgacctgaa tctgaaagat
      901 cttggagtcc tttcgccatg tcgccgccca gtcgattcca ataaacgtct cgcgcttcgc
      961 gaaaacggtc tcggtacatt tgtgcagtat catggaatgc aggcaaccaa cgcgtgcgcg
     1021 caacctctcg ttctgccagc tctacaaggt tattcatgta gtcggagaat ctctgctgga
     1081 gcagcggatc gcggaacatc gaaagcaatg tgggcgaaac cgatattgtt attcggtaag
     1141 gaacctcttc atcggccagt cgcgagagga ctatgtaaag agggatatag gtttcaatta
     1201 atgcttcgta gaaccatttt tcttccaggt aatgttcgcc gtcggcgtgt cgcacgaagg
     1261 gaagatgcgc atgcaagaca agagcaaggt agccatttgg agttgacaac aactacgacc
     1321 tcccatttcc ccggttcgat ctggagacaa tccacgaaac gcttctatgc atgatgccgt
     1381 catcggatgt ggcggaaatg tcaaccgggt ggtctccaaa tggcagagac aggcggactg
     1441 agaaactgcc atcaggtctg acaggcatcg gcatgccgca tacttccact ttagcccctg
     1501 cttccgttgc tccgtgaatg atcagttcgg catcggctct caaccagaag ccgcgtgggg
     1561 ctcggtgtac ttcgccaggt tggcccatag ggcttccgac tgctcccact cctcccgatc
     1621 cgagggcaat ttcgtgaagc agttcttcgg cgcgggcatg cctggaaata cccggcgatg
     1681 aaggcgatag gccatgcgat atgtaacctt cctcaatctc gcgaatggtt agccactctt
     1741 cgtcgacaat ccaggatgga cctatccttg gtgtgtggac ggtattcgac cgcgagatca
     1801 ggtagaattg tgaggcgccc gtaaccagcc ccaggtcaac acagtaggac ctgtctgatt
     1861 cctccacgtt tatgtaccaa ttgttcgcga aaggatggac ctctttgtcg aaactgcgga
     1921 cggaattatg gccatcgaat gtgacgcctg ttacatcgtg gacgcggagc gtcaactgtg
     1981 agcggtccaa ctcttctccc aactggcggc gtaggttttt ggccatgtct tcggcgattt
     2041 cccaatatgc gtgcatccag aagggatcgc gaacttgaag cactattctg gtagccccgt
     2101 agaaacccgg caagaccgtg ggtttgtggc gccgagcggg gttcatctgt aagttttcgg
     2161 tctcaaggat cgtgatctcc gggaagcgtc tgtgtggaag cggctgatcg ctcacttcgg
     2221 gcggactcag ttcgattctg gcatcttccg ccatctgctg ctcgtgctgg gacacgctgg
     2281 gcgtccctgc gccttttttg tctatcggac taactttatc gaacgagtcc tgcacttgtg
     2341 gaacctccct ttaagcgcca aaacacataa ggctcaacgg ctggtgttgg ccgtctgtca
     2401 atctctcgct tagtctgccc gcgagaaatg cgggttatcc cataccggat actcttctcg
     2461 ctttgggcaa caatagttta tgcttcaagg aggtatttac tatggaccac attggaacgt
     2521 tttctctggt gcttcattct cacatcccat attgccgcag atccggagtg tggccatttg
     2581 gagaggagtg ggtttttgaa gcagtggtgg agacttatat ccctatactg gattggctcc
     2641 tgaattgcca tgaaaaaggg gtcggaccca atgttacgct cagtttttcc ccggtattgg
     2701 cagagcaact tgcggatgca tacatgaagt cccgcatcgt agaatacatg gacgctcgcg
     2761 ccaatgcggc ccgcgctgat ctcatgcgac acgagttagt ccacgacgac aggcttgcaa
     2821 ggctgtcaag gaagtacgtc gaatactatg aaaacgctaa gaaacgattc atagattact
     2881 acgacggcga cttgttgggc gccatcgcca agcttgccgc ctcgggtaga gttgaggtct
     2941 tggctacatc ggccagccac gcctatctgc cattgctgcc tcaagaatca cgtattcgca
     3001 ctcagattcg cgtcggttac gaggcatgcc aacggcactt tggcgttttg cccaagggcg
     3061 catggcttcc cgaatgcgga tacaagcctg gcgcaatcga gcgcgcgctg gaggaatgtg
     3121 gaatcgagta tttcatagtc gatactccag caatagctgg cggtggcccg cgaaacctgt
     3181 atggtggcgt tcctgaggct ttgcggcgca acattggggc tggcttgccg agtatggcaa
     3241 cgctcatgcc gtatcgcctg cgtggctcca agatggctgt tttcgggcgc gatgaggtga
     3301 cagctgctca ggtatggtcg agggatgtgg ggtatccggg tgacggcaat tacctcgaat
     3361 tccacaagcg ggacgactca tccggacttc gttactggag ggttacaaac aaactcctgg
     3421 atttcgggca gaagcagctt tacgaccctg aggctgctct agcaagcgca agaacccatg
     3481 cacagcactt cgctggtgta gtagaggaaa ggcttggtgg ttacgcagag aggacaggca
     3541 ggcatgggat tattgttgcc ccctacgaca tggaactttt cgggcattgg tggcatgaag
     3601 gcgttgattg gctttcattg gcaatggacg cgctctgtgc atcgaatcgc gtcgagtgtg
     3661 gcaccctggg atcttacttg aagctgcacc cgcccgtgga tgaactggag ctccctgagt
     3721 gttcgtgggg gcatggcgga agacatggtg tatggttcaa ctctgaaacg gaatggatgt
     3781 ggcaggagat tgagagagcc actgctatcg gccgcgagct tgaaggggta attgggggcc
     3841 tagacggggt ccataaaatc atggccgatc aggcgcttcg agagctgatg ttgttggaag
     3901 cgagcgattg gcccttcctc gtgacaacgg gtcaagctgg aggatatgct cgtgataggt
     3961 tcgcgcagca tctgtccgat tttgaggttc tggcgcaggc agcgatggct agggaaaccg
     4021 atctgcgatc gctggaggtt ttggaagcat cgtcttcccg cgattcggtc tttacatgga
     4081 tcgactgggg aagcctgtga tatactcgga agtacaaact gagtatgcga cggggttttt
     4141 atgcccgggc tgatctctcc gtctggccct gtcttctgca gatctggatc actgttagag
     4201 atcagcttgt ttcgaacttt caatcacatt ggagttgtcg ctgaaattaa cgagtgtgac
     4261 ggagatgtga ttgtttcctt acgaggtttg a
//
LOCUS       DAOSAI010000208         4288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12953_length_4288_cov_3.578077, whole genome shotgun sequence.
ACCESSION   DAOSAI010000208 DAOSAI010000000
VERSION     DAOSAI010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12953_length_4288_cov_3.578077"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLM83_07405"
     CDS             complement(<1..538)
                     /locus_tag="PLM83_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOK71266.1"
                     /translation="MQHIWNATLLGLAAGVLGTGFGAFLTAASGTPKRRTLSTLMGTA
                     AGVMLSIVFLDLIPEAIEIGGVAQTVGGFLLGVGATGIMDLLIPHFHFVSEDCKQNRF
                     LKAGLLTGIGIAMHNLPEGLAIGASYAHGNSLGLSVALTIAIHNIPEGMAMAAPMCAG
                     GVGRKQSTLYGALAGLPMA"
     gene            669..1436
                     /locus_tag="PLM83_07410"
     CDS             669..1436
                     /locus_tag="PLM83_07410"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOK71267.1"
                     /translation="MILAIDVGNTNTVLGVFDGEELLADYRVSTDRQRTADEWGMLLL
                     ELLDHAKIDGAAISGVIISSVVPPIITELAKMSRRYFRCDPEVVGPGMKTGISIRYEN
                     PKEVGADRIVNAVAGYELYGGPLIIVDFGTATTFCAIGQTGDYLGGAIAPGIMISAEA
                     LYMRAARLPRVELTKPQSVIGKNTVSSMQAGILYGFVGQVDEIVRRIKQELGGDPKVI
                     ATGGLAEVISLESDHIDEVNQNLTLIGLRILWERNHS"
     gene            1452..2777
                     /gene="hflX"
                     /locus_tag="PLM83_07415"
     CDS             1452..2777
                     /gene="hflX"
                     /locus_tag="PLM83_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOK71268.1"
                     /translation="MLNTSFSLEGANNSSLHSGSGDNMERAVLVGLETAAPSAWNIED
                     SIGELAHLTETAGAAVVATVIQKKGKPDPAYYVGEGKAREIEMVRSATEANLVIFDDE
                     LTPAQQRNLEEIIEGRIIDRTELILDIFAQRAATSEGKLQVELAQLNYMLPRLAGRGT
                     EMSRLGGGIGTRGPGETILEADRRTIRNRISSITRELETVRQRRTVQRQKRRDADLPL
                     VALVGYTNAGKSSLLNTLTGAEVLVEDKLFATLDPTTRRYTLPDGQALLLTDTVGFIQ
                     KLPHHLIMAFRATMEEVVDADMLLHVVDASHPQAVEHCEAVFDVLREIDAADHPVITV
                     LNKSDLPESGPMIERLHHAYPKSVAVSAVTGDGLDDLAEMISEELSSWRVRIEELVPY
                     GDPVLGLIRARGRVISEVYREDGVAIVAEVDRMVAGQVRKARSNGSKRQ"
     gene            2827..3795
                     /gene="dusB"
                     /locus_tag="PLM83_07420"
     CDS             2827..3795
                     /gene="dusB"
                     /locus_tag="PLM83_07420"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOK71269.1"
                     /translation="MGGVRLMNPLVLAPMAGVSDAPFRRICKEMGCALVYTEMISGMA
                     LVYRNRRTLDMLHVSEDEHPIAIQLFGSDPRVMASAARIVMDGGADVIDINMGCPVPK
                     IVNNGEGSALMRDPERAWAIVAAVRQAVDVPVTVKIRKGWDDSSVNAVEFAKGCVDAG
                     ADGVAVHGRTREQGYSGDADWNIIAQVAHAVDVPVIGNGDVISAENSFEMLESTGCAG
                     VMIGRGALGNPWIFRDCLHYLATGTHLPQPQPRDRISMALRHLDDAVDLKGEKVAVRE
                     MRKHMAWYIKGLRGAARIREAINASEDAEALRSIMSAYLKQLEDGV"
     gene            3990..>4288
                     /locus_tag="PLM83_07425"
     CDS             3990..>4288
                     /locus_tag="PLM83_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOK71270.1"
                     /translation="MTNLIRIGDKVVDRDRIDRALDKLFDMRQNGASQAEVAAALGTD
                     RSFVSRLENLGEIRKGERVAVIGFPIGNKEEIEQLAKKAGVDYVWLMNDEERWAYV"
BASE COUNT          975 a         1027 c         1320 g          966 t
ORIGIN      
        1 acgccattgg cagacctgcg agggcgccat atagtgtgct ttgctttctg ccaacgcctc
       61 ctgcacacat gggtgcagcc atggccatgc cttccgggat gttgtgaatg gcaatggtga
      121 gagccactga aaggccaagc gagttgccgt gagcgtaact cgctccaata gccaacccct
      181 ccggcagatt gtgcatggca attccgatcc ccgtgagaag ccctgctttg agaaaccgat
      241 tctgcttaca gtcttctgag acgaagtgga aatggggtat cagaagatcc atgattcctg
      301 tggccccgac gccgagcaaa aaaccgccta ctgtctgcgc aaccccgcct atttctatgg
      361 cttcggggat aaggtcgagg aagactattg acagcattac tccggcggct gtccccatga
      421 gcgtcgagag agtgcgccgc ttgggagtcc ccgatgctgc tgtcagaaaa gcgccgaacc
      481 ctgtgccgag gacgcccgct gcaagcccta gaagtgttgc attccagatg tgctgcaatt
      541 tagcacctcc tgctcttagc atattctacg tccatgcacc tcccatgtca aaaccgaatg
      601 tacaggagat tagggcacat gatggacgag atcgctgtat ggtataattg cgcggagagg
      661 gtggtcggat gattctcgca attgatgtgg gcaataccaa tactgtgcta ggtgtattcg
      721 atggcgaaga actccttgca gactacagag tatccacaga caggcaaagg actgctgatg
      781 aatggggcat gcttttgctg gaattgcttg accatgcgaa aatcgatggg gctgcgattt
      841 ccggagtgat tatctcgtca gtcgttcctc cgataatcac ggaacttgcg aagatgtcgc
      901 gcaggtactt tcggtgcgat cctgaagttg tagggccggg gatgaagacg ggaatttcca
      961 taagatacga aaatccgaaa gaagtggggg ccgacaggat cgtaaatgcc gttgccgggt
     1021 acgagctgta cggcgggcct ctaattattg tagatttcgg caccgccacc actttttgtg
     1081 ctattggtca aacaggcgat taccttggcg gcgctatcgc ccccgggatt atgatatcag
     1141 ctgaagcttt gtatatgagg gcggcccgtc ttcctagggt ggagttgacg aagccgcagt
     1201 ctgttatagg caagaacaca gtttcaagca tgcaggccgg aatcctatac ggcttcgtgg
     1261 gccaggttga tgagatcgtc cgaagaataa agcaggagct tgggggcgat ccgaaagtga
     1321 ttgccactgg agggcttgcg gaagtgatat ctcttgagtc agatcatatc gatgaggtca
     1381 atcaaaactt gacgctaata gggctgcgca ttttatggga gaggaatcat tcttaggctc
     1441 agcggggtga tatgttgaat accagtttca gccttgaggg tgcaaacaac agctcgttgc
     1501 attccggatc aggcgacaat atggaaaggg cggtgttggt gggcctggag accgccgctc
     1561 cttctgcgtg gaacatagaa gactccatag gagaactggc acatttgact gaaactgctg
     1621 gggcagccgt ggtcgccaca gtcatccaga agaagggcaa acctgatccc gcttattacg
     1681 tgggagaggg caaagctcgg gagatcgaga tggttcggtc ggcgacagag gccaacctcg
     1741 tcatcttcga cgacgaactg actcctgcgc aacagcgaaa tctggaggag atcatagaag
     1801 ggcgaataat cgacaggact gagctgatcc tcgatatttt cgctcagcgt gctgcgacat
     1861 ctgaaggtaa actccaggtt gagttggcgc aactcaatta catgcttcca aggttggctg
     1921 gccgagggac agagatgtcg cggcttgggg gcggtatcgg tacccggggg cctggagaga
     1981 ccatacttga ggcggaccgc aggacgatca gaaaccgtat atccagcatc acccgcgagc
     2041 ttgagacggt caggcagcgc cgcacggtgc agcggcaaaa gaggcgtgat gccgatctcc
     2101 cgcttgttgc gttggtgggc tacaccaatg caggcaagtc aagtttgctc aacaccctta
     2161 ccggggctga agtccttgta gaggacaagc tctttgccac cctcgatcca acgactcgtc
     2221 gatacacgct ccctgacggg caggcgctct tgctcacgga taccgttggt tttatacaga
     2281 aactgcctca tcatttgata atggcgttcc gggccacgat ggaagaggtc gtggatgctg
     2341 atatgctgtt gcacgtggtc gacgcttctc acccgcaggc agtggagcat tgcgaagccg
     2401 tctttgatgt attgcgcgag atcgatgccg cggaccatcc ggtcatcact gtgctcaaca
     2461 aatcagattt gcccgaatcg ggcccgatga tagagaggct gcatcatgcc tacccgaaaa
     2521 gtgttgccgt ttccgcagta acaggcgacg ggctcgatga tcttgctgag atgatatcgg
     2581 aggagttatc cagctggagg gtgaggatcg aggagctcgt gccctacggt gatcccgtgc
     2641 taggactgat cagagcaagg ggcagggtaa tctctgaagt gtatcgcgag gacggcgttg
     2701 ccatcgtggc agaagtagat cgcatggtcg caggacaggt aagaaaggcg agatccaatg
     2761 gctctaagcg ccagtgactc ctcttttcgg cgggagcccg attccgttct tgccgatggt
     2821 tttgccatcg gcggcgtgcg cttgatgaat ccgcttgtgc ttgcgccaat ggctggcgtg
     2881 agcgatgccc ccttccggcg gatatgcaaa gagatgggct gtgcccttgt ctatacggag
     2941 atgatcagcg gcatggccct agtatatcga aatcggcgca ccctggacat gctgcatgtc
     3001 tcggaagacg agcaccccat tgccatacag ctcttcgggt cggatccgcg tgtaatggcc
     3061 agcgcagctc ggattgtgat ggatggcgga gcagacgtga tcgacatcaa catgggatgt
     3121 cctgtgccta agatcgtgaa caacggtgag ggttcggcgt tgatgcggga tcctgagaga
     3181 gcgtgggcta ttgttgcagc tgtgcggcag gcggttgacg ttcctgttac agtcaagatc
     3241 cgcaagggat gggatgactc cagcgtgaac gctgtcgagt ttgccaaggg ttgcgtggat
     3301 gctggggcgg acggggtcgc cgttcatggc cggacgcgtg aacagggcta cagtggcgat
     3361 gctgactgga acataatcgc tcaagtcgct catgcggtgg atgtgccagt gatcggcaat
     3421 ggggacgtaa tcagcgccga gaattctttc gaaatgctgg agagcacggg gtgcgccggg
     3481 gttatgatcg gccgtggggc actgggtaac ccttggatct tccgagattg tctgcattat
     3541 ctggctacag gcacgcatct tcctcagcct cagccccgcg ataggatttc tatggcatta
     3601 cgccatctcg atgacgctgt cgatctcaag ggggaaaagg tagccgtgcg cgagatgcga
     3661 aaacacatgg cttggtatat taaagggctt cggggtgcag cccgtatccg cgaggcaata
     3721 aatgcttcgg aggacgctga agcattgcgt agtattatga gtgcttactt gaaacaactt
     3781 gaggacggcg tttgaagcag tttcgtgggc aattgactgt tgcggaggga atgtcgcctt
     3841 tgaccgcctc atgcctgggc ggtgttttgt tctgcgtctg tgcaaggaaa cacccgaaaa
     3901 acacttgttt gcaggtgcgt cctctggtaa aatcggtgtg tgcacacaaa tgtgaacatt
     3961 cctacgtctg tcttctgcgg ggggtgggct tgacgaatct catacgcatt ggcgacaagg
     4021 ttgttgacag ggacaggatt gacagggccc tcgataagct gtttgatatg cgacagaacg
     4081 gagcgtctca ggctgaagtg gctgcagcct taggaacaga tagatcattc gtgtcgcgcc
     4141 ttgagaatct cggcgagatt cgaaagggcg aacgagttgc ggtcatcggc tttccaattg
     4201 ggaacaaaga agagatagaa caactggcga agaaagctgg ggttgactac gtctggctca
     4261 tgaatgacga ggagcgttgg gcttacgt
//
LOCUS       DAOSAI010000209         4285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12970_length_4285_cov_3.651064, whole genome shotgun sequence.
ACCESSION   DAOSAI010000209 DAOSAI010000000
VERSION     DAOSAI010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12970_length_4285_cov_3.651064"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLM83_07430"
     CDS             <1..642
                     /locus_tag="PLM83_07430"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOK71271.1"
                     /translation="CFMPFGEMTVQSFLDELASNSPAPGGGSAAAVAGAMGAGLVSMV
                     AGLTETAEMSEEDAEKVRQAGARAQKLRTELLRCADEDTRAFNEVMAAYRMPRGTDEE
                     KQTRREAIQQGLKGAVAVPMRTLELAMEGLGLATYMAFEGNKNAVSDAGVSAQLFNAA
                     VRGAIFNVEINLSGIKDADFVSEARAKVASYDLERERLRKESLDAVLGRLGGN"
     gene            857..2164
                     /gene="folP"
                     /locus_tag="PLM83_07435"
     CDS             857..2164
                     /gene="folP"
                     /locus_tag="PLM83_07435"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOK71272.1"
                     /translation="MNTDMDNVDNEQNCGSDWPVRCGTGCNHDVRVAVLPCKEDVEAE
                     LLKMGASPAGIEIMAEKGVFRTVILRNVPIKAALIIKQEMLAKGGEAALPHSAIGLCA
                     DSCDILLMGTLRQYERVIHTLALQPFGLLGIAREIQCALASYDGRPESICIGGRTFLY
                     GERTYIMGILNVTPDSFSDGGAYTTADSAFEHAMEMLEAGADIIDIGGESTKPGVDPL
                     PLDEELERVLPIVKRLASQTDAVISIDTYKAEVAERCLDLGAHIINDVSALRGEGDMA
                     SVVSRYNAPVVLMHSKGRPTHMQADPTYECVVREVYSFLAERMEYACERGVSPCNIIL
                     DPGIGFGKTPQHNLELMRRLAEFKSLGRPILVGTSRKSTIGKVLHKPVSDRVWGTAAT
                     VATAIMSGADIVRVHDVREMAMVAQMTDAICRGWRGEEDGHES"
     gene            2154..2570
                     /gene="folB"
                     /locus_tag="PLM83_07440"
     CDS             2154..2570
                     /gene="folB"
                     /locus_tag="PLM83_07440"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOK71273.1"
                     /translation="MRADRITLKNLVFYAYHGVFAAEKELGQKFEVDVDMRGDFQAAA
                     RVDDIDMAVNYADVYATVKDLVEERDFDLIEALALNIADEVLSGYEVEEVTVRVRKPS
                     APIGGPADYVECEITRSRSDMAPLGEEDDGLDDPAL"
     gene            2554..3348
                     /locus_tag="PLM83_07445"
     CDS             2554..3348
                     /locus_tag="PLM83_07445"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOK71274.1"
                     /translation="MIPHFRIERLDEVDSTNSYARALAEAGAPDGTVVVAERQTAGRG
                     RLGREWHSSGDFGLWFSLLLRPEFEPQFAGMLGIAAGVAVVQSMRSLVGARAVLKWPN
                     DVLIDGRKVCGILSEGRTVGQKLEWALIGVGINVTAPEGGFPSELEDVATSLAQHCAA
                     DIGAPAPDKEALLDSVLSQFGARYAMLSSGNVRAVRDEAEELMRSFLNRRVTIRGQSG
                     TMEVRTLDLSEDGALLVELDGGEVAKITAADVSIGTRACARGELDA"
     gene            3341..3964
                     /locus_tag="PLM83_07450"
     CDS             3341..3964
                     /locus_tag="PLM83_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOK71275.1"
                     /translation="MHNAIALAIAALMVGMSCNSPATAGISGQEGIQVVSPVSVSAGG
                     AGTSALDWTALLRAVTLLGGPEGTLVLSAALLTADPPTGEMVIDASVSTALVVEVLKV
                     GFGRARPYVPGENGRFTGPNLSGKYHSMPSGHSANAFAIATVLAHRYPEQAALAYGLA
                     LAVGLSRIQLGVHWPSDVVAGAAIGLVMGNQVVEGRLHLLELDGATD"
BASE COUNT          938 a         1014 c         1374 g          959 t
ORIGIN      
        1 tgctttatgc catttggtga gatgactgtc caaagctttc ttgatgaact cgcttcgaat
       61 agtcctgctc ccggcggagg aagcgctgcc gcggtggctg gggctatggg cgccggtttg
      121 gtgtcgatgg ttgcaggcct taccgaaacc gcagaaatgt cggaggagga tgctgaaaag
      181 gtcagacagg ctggtgccag agctcaaaag cttcggacag agcttttgag gtgcgctgat
      241 gaagatacta gagcgttcaa cgaggtaatg gcggcgtaca ggatgccgcg ggggactgac
      301 gaggaaaagc aaactaggcg tgaagcgatt cagcagggac tcaagggcgc cgtcgctgtc
      361 ccgatgagaa ctttggaact ggcaatggag ggtttggggc tggcaactta catggcgttt
      421 gagggaaaca agaacgctgt gagcgatgca ggagtttcgg cccagctttt caatgcagct
      481 gtccgtggcg ccatcttcaa tgtagagatc aacctatcgg gcatcaagga tgcggatttt
      541 gtctctgagg cccgagccaa agtcgcctcg tacgacctcg aacgggaaag attgaggaaa
      601 gagtctttag acgctgtttt aggcagactt ggggggaact gaaaaccact caaggacgtc
      661 gatgtagaga ggggctgtgg ctaggcgagg atcggccttg cctgaatgga taagttttac
      721 aggctaaagc cgaagcgtgg ggcgggagca agttggcaag gcatatggat cagcctgtga
      781 ttagtttggc atggtggcgc ttgccttgta gcttggatcg ggcgagcaca tttatgacta
      841 tggaggactg cagctgatga acacagacat ggacaacgta gataacgagc agaattgtgg
      901 atcggattgg cctgtccggt gcgggaccgg gtgcaatcat gatgtgcggg tggctgtgct
      961 gccctgtaag gaagatgtgg aagcggagct tcttaagatg ggtgcaagcc cggccggcat
     1021 cgagatcatg gccgaaaaag gcgtttttcg gacggtgata ttgcgtaacg tgccgatcaa
     1081 ggctgctctg ataatcaagc aagaaatgtt ggctaagggc ggcgaagctg cactcccgca
     1141 ttccgccata ggtttgtgtg ccgactcatg cgatattctt ttgatgggca cattgaggca
     1201 gtatgagcgc gttattcata ccctagctct gcagccgttt gggcttttag gcattgcacg
     1261 ggagatccag tgcgcgttgg cgagctatga tgggaggcca gaatctatct gcatcggagg
     1321 aaggacattc ctttacggag aaagaacata cataatgggc atccttaacg tcactcccga
     1381 ctctttctct gatggcggag cgtacactac agccgattcc gcattcgaac atgctatgga
     1441 gatgcttgag gccggcgccg acataatcga catcggcgga gagtcgacca agccgggcgt
     1501 agatcccctg cccctggacg aggaacttga aagggtgctt cctatcgtaa agaggcttgc
     1561 atcccaaaca gatgcagtta tttcgattga tacatacaag gccgaagtgg cagagcgctg
     1621 cctagacctc ggcgcgcaca tcatcaacga cgtgtccgct ctccgaggag agggtgacat
     1681 ggcgtcggta gtctcgcgtt ataatgcccc tgtagtgctg atgcactcaa aggggcgtcc
     1741 cacacatatg caggcagatc ccacgtatga gtgcgtggtc cgcgaagtat acagtttcct
     1801 tgccgagcgg atggagtacg cgtgtgaacg tggcgtttcg ccctgtaaca taattctaga
     1861 tcccggaata ggctttggta agaccccgca gcataatctc gagttaatgc gacgtctggc
     1921 ggaatttaag agtctgggca gacccatttt ggtgggcaca tccagaaagt ccaccatagg
     1981 aaaggtgttg cacaagcctg tgtcagatag ggtttggggc acggctgcca cagtcgccac
     2041 ggccattatg tccggagcgg atattgtgag agtccatgat gtgagggaga tggctatggt
     2101 tgcgcagatg actgacgcaa tatgcagggg atggcgcgga gaggaggacg gccatgagag
     2161 ctgataggat taccctgaag aacctggtgt tctatgctta ccatggtgtt ttcgcggcag
     2221 agaaggagct gggacagaaa ttcgaggtag acgtggatat gagaggggat ttccaggcag
     2281 ctgctcgtgt tgacgacatt gacatggctg tgaactatgc ggacgtgtac gctacagtga
     2341 aagacctcgt ggaggaacgg gatttcgacc tcatcgaggc gcttgccctg aacatcgcag
     2401 atgaggtgtt gtcaggctat gaggttgaag aggtaacagt aagagtacgc aagccgagtg
     2461 ccccgatagg cggcccagcc gactatgtgg agtgtgaaat caccagatct aggtcagaca
     2521 tggcgccgct cggagaggaa gatgatggcc tggatgatcc cgcactttag gatcgagcgg
     2581 ctcgatgagg ttgattccac aaactcgtat gcaagggcac tggctgaagc cggagcgccc
     2641 gacggaacag tcgtggttgc cgaaaggcag acggccggca ggggaaggct gggaagagag
     2701 tggcattcat cgggcgactt cggcctgtgg ttttctttgc ttctgaggcc cgaatttgaa
     2761 ccccaatttg cgggaatgct tggaatcgcc gcaggagtgg ctgttgtgca gagcatgcgc
     2821 tcgctggtag gcgctagagc cgtcttgaaa tggccgaatg acgtcctcat agacggccgc
     2881 aaagtatgcg gaatactctc cgaaggtcgc acggttgggc agaagctcga gtgggcattg
     2941 attggagttg ggatcaatgt tactgctccg gagggcgggt ttccatccga gctagaggat
     3001 gttgctactt cattggccca gcattgcgcc gctgatatcg gtgctccagc accggacaag
     3061 gaagcgcttc tcgactctgt cctcagccag ttcggggcga ggtatgccat gttaagcagc
     3121 ggaaacgttc gtgcagtccg cgatgaagcg gaagagctaa tgagatcttt tttgaaccgc
     3181 agggtcacca taaggggtca gtccggcacc atggaagtca gaacgttgga tctttctgag
     3241 gatggcgcgc tcctggttga actggacggg ggagaagttg cgaagataac ggcggcggat
     3301 gtgtcgatcg gaacccgcgc ctgtgcccga ggtgaactcg atgcataatg cgattgcctt
     3361 ggctattgca gccctcatgg taggcatgtc ctgcaattca cctgcgacgg caggcatttc
     3421 cggccaagag ggtatccagg tggtgtcccc cgtgtctgtt tcggcaggcg gagcgggcac
     3481 atctgcgctg gactggactg cgctcttgag ggccgtcact ttgctgggcg ggccagaggg
     3541 gacgttggtc ctcagcgctg ctcttctcac cgctgatccg cctactggcg agatggtgat
     3601 cgacgcttcg gtatccaccg ccttggttgt ggaggtgctc aaagtagggt ttgggcgcgc
     3661 aaggccatat gttccgggag agaacggacg tttcaccggg cccaacttgt caggaaaata
     3721 ccattcaatg ccttctggcc atagcgccaa cgcatttgca attgcgacgg tactcgccca
     3781 tagatacccg gagcaagcgg cgcttgcgta tggccttgca ttggcagtgg gactcagcag
     3841 aattcagctc ggggtccatt ggccatcaga cgtggtggca ggagcggcga tagggctagt
     3901 catgggaaac caggttgttg agggacgttt gcacctgctc gagctggacg gagcaacgga
     3961 ttgacgggca tacgaatggg gcaggtgtgc gccgtcgcat cctgtaggat gacctcgagc
     4021 gatgggacga gatagggaca gccgggccgc ttcgcaaagc gcccggctgt cttcgtcatt
     4081 tcctgcctgt ttttagttgc cctgcccctc tgcattgagc cggcttcatc cctgctcttt
     4141 cgtttggacg tcgctcttgc attgattttc ggacactggc tatcgctttt gccatcatcc
     4201 cgagcccgtt actctcgcag aattattcat ctggtgctcc caatcggtta gtggtgatag
     4261 cagatctact cgtccgcgtt ctctg
//
LOCUS       DAOSAI010000210         4278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_12998_length_4278_cov_4.318968, whole genome shotgun sequence.
ACCESSION   DAOSAI010000210 DAOSAI010000000
VERSION     DAOSAI010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12998_length_4278_cov_4.318968"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            555..2603
                     /locus_tag="PLM83_07455"
     CDS             555..2603
                     /locus_tag="PLM83_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71276.1"
                     /translation="MSKSSLVVNVKQKVLIAITVIGVIVSGTMWVRQNLAGMASQAVS
                     LGISRVLGLSDVRIGHVTARGIRSIDLAGVCIGDEVSIDHLLVDYDVSDLFFGRAGLI
                     ESITGVHARGVSVDIAAPLVHEPANRGHVERKSGARSDGGTKTELSRFSAPIEICDVL
                     IEFPSEDQGFRKLEVAKVTVESTLLDGCGVWKVALSNATYTDGTAFDIEGAKAFVDIR
                     GETVELSDIDAQLLGGSVRGRLVLCPSVDGPLFLTGSLDIADLCCGESTISGYVRMDE
                     DGAVAARGSVKLPQAGIDGFLEAEYEGCLRAPGVEIGTRNEARSDARPAAEAGSAYEA
                     HGVLHLVDLSVGEHRASDIDLDLDASYGGGKIKAAIGGERMSMERVGKALGRAIPVEG
                     WVDWKADIEFEDSTLAAVVTIRDGDLRSELWPQEFTDLSGSIEVAEAIPNNGAGSLEE
                     VRVRTIRAAMGGGTVELSGGGQMWTIHGLNLEIEHPLLCGLVDIDATIHTGERMKVGG
                     ILSLCNADVDVVTAGFSKAAVIPDADLDIKIVVGDGVKAVREESWAEIMPSVVQIQGS
                     VRQPRAVGYVQVGPGEVYICDIPLEILSGRVEFANTPDSDIDFSVVARDSSAEFPILA
                     TIYGRPGDVHLSFEPASGVEENFPSGELLMKLLDARLRLYILSRLGQLVGALPQASRD
                     "
     gene            2740..>4278
                     /locus_tag="PLM83_07460"
     CDS             2740..>4278
                     /locus_tag="PLM83_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOK71277.1"
                     /translation="MKDTSTKRSCCILAAAMAVVFLASSFTMAAQSKSLGVVTAIEVI
                     GNSRIDSDIITQAINIKVGDELTESAIRESFTAIDALGWFADLGANTVPYLGGVKLII
                     VVQEFPVLSKILVSGNALIPTEELLGIMGSKVGSQLNANQLRSDLEALGSHYHDKGYW
                     VSIEPSLSESGELILHLVEWTVSEVKFAGNQKTKPKVIQRCIETKPGDYINVNKINED
                     RRRIYMLGAFENVEAKVEPVPGKYEYVVTYVLTERKTGIANLALTYSSQDKLMGYMEL
                     GDENFLGNAQTVNIKAEFGRTKTNYELGFYEPWVGASGKTSIGFNVFSKTLSKKYTPS
                     DENETSSSGQVLKLEEDPTASIDYSQKRTGGDVTIGRTLSLNTKAFVKLKMENAVNDP
                     EQKHPDVDFPGGGATRSLTLSAVNDTRDDLWNPATGHKLSSSLEYAGGILGGDNNFAK
                     LRVDACQYYQVRDGHILAGRVSAGYGLDELPDDERFRLGGAETVRGYKYGDIEGDRMI
                     FANAE"
BASE COUNT          984 a          969 c         1311 g         1014 t
ORIGIN      
        1 cccatggggg aaacgatttt ttagccgcct ttcaaacccg cgttatcgtg ggcttccgtt
       61 acatcaaaaa tggctcttct tctcagacta gccgggcaaa actccaatgc ccatgaatgg
      121 ggcggcctcc aagtcggcct ctgagtttgc ggacgcatgc agcctgcaac ctcctagctc
      181 cctgcttctt gcggaatgct gctgacccct tgtgcttgtt tatgctgcgt aagtcccgtt
      241 cgccaatcaa aaaagtcgtg acacatgact atttggttgc ctggaagacg ccacctgcag
      301 atgagcaacg tttgttgaag gccgacgcgg tttgttgctt ccttgaacgg gtgaacatag
      361 cgatcaaatg tgttttcaat cttcgccgag tagcgcgccc gaccggggct gccctccatt
      421 atgtgtaatg tatgacagac aagatgtgaa ctgatgggga aacccctggg cgggttgagg
      481 ttgggaataa gagaaaaccc tgcattgtgc cccggacttt cactgcctat gagtcgaagg
      541 gaaggatgag tcctttgtcg aaatcgtcgt tggtggtaaa tgtgaagcaa aaggttctaa
      601 ttgccataac agtgatcggc gtgatcgttt ccggaacgat gtgggtcagg cagaatttgg
      661 ccggcatggc gtcccaggcg gtcagcctcg gcatctcacg cgtgttgggc ctctcagatg
      721 ttcggatagg gcacgtgacg gcgaggggca tccgttccat agaccttgca ggtgtttgca
      781 taggtgacga ggtcagtatc gaccatcttc ttgttgacta cgacgtatct gatctctttt
      841 tcggtcgggc cgggttaata gagagcatta ccggtgtgca cgctcgcggc gtcagcgtcg
      901 acatcgcagc gccgttagtc catgaacctg caaaccgcgg ccatgtggag agaaagagcg
      961 gtgcgcgttc tgatggcggg acgaagacgg aactcagtcg gttcagcgct cccatcgaga
     1021 tctgtgatgt tctgattgag tttccatcag aagaccaggg tttccgcaag ctcgaggttg
     1081 cgaaggtgac cgtggagagc acattgctgg atgggtgcgg tgtctggaag gtcgcgcttt
     1141 caaatgccac gtacactgat ggtacggcgt ttgatattga gggggcaaag gcctttgttg
     1201 atatccgcgg agagacggtg gagctttccg acatcgatgc gcaactccta ggaggcagtg
     1261 taaggggaag attggtcctg tgtccaagcg tagatggccc tttgtttctc actggaagtc
     1321 ttgacatagc ggatctgtgt tgcggggagt cgacaatctc cggttatgtt agaatggacg
     1381 aagatggagc tgttgcggct cgtgggagtg tgaagcttcc gcaagctgga atagatgggt
     1441 ttcttgaagc agaatacgaa ggctgcctgc gcgcaccggg agtggagata ggaacaagga
     1501 atgaagctag gagcgatgcg cggcctgctg ccgaggcagg cagtgcatac gaagcccatg
     1561 gagtcctcca cctggtcgac ttgagtgtcg gagagcatcg ggccagcgat atagacctag
     1621 acttagatgc aagctatggc ggagggaaaa tcaaagccgc tataggcggg gaacgcatgt
     1681 caatggagag ggttggcaag gctttggggc gtgctattcc agttgaagga tgggttgatt
     1741 ggaaagccga tatcgaattt gaagattcga cgctggccgc tgtggtgact attcgtgatg
     1801 gcgatctcag atcggaacta tggcctcagg agttcacgga cctaagcggt tcgatagagg
     1861 ttgctgaggc tataccgaat aatggtgcag gttcgctgga agaggtcagg gttcgcacga
     1921 ttcgagctgc aatgggaggc ggaacagtcg agctctccgg tggaggacag atgtggacca
     1981 tacatggctt gaatttggaa atcgagcatc cgctgttgtg cgggttagtc gacatagacg
     2041 ccacaatcca caccggagag cggatgaaag tcgggggcat tctcagtctc tgcaacgcgg
     2101 atgttgatgt ggttactgct ggattttcca aggcagctgt gattcccgat gccgaccttg
     2161 acataaagat cgttgttggt gatggagtga aggccgtaag ggaagaatcc tgggctgaga
     2221 tcatgccctc tgtagtgcag atccagggaa gtgtgaggca accccgggct gtcggttatg
     2281 tacaagtggg acccggggag gtgtatatct gcgatattcc tctggagatt ctgtccggga
     2341 gggtggaatt tgcgaacact ccagattcag atatcgattt ttctgtcgtt gcgcgtgatt
     2401 cctctgctga gtttcccata ctggccacga tatatggaag gcccggcgat gttcacctgt
     2461 ctttcgagcc agcgtcgggc gtggaagaaa actttccgtc gggggagctt ctcatgaaac
     2521 ttctggacgc ccggttgcgt ctgtatattc tgagccggct tggccaactc gtgggcgcgc
     2581 tgcctcaggc gagccgggat tgattgccat tcccgggctt tgaggcggga ggaatcgcta
     2641 ggcgagggta gaatactgaa tccaactcat cagtttatga tggtggcctg agcgggctgc
     2701 agtcgtggcc gaacagcgcg atttggggag gcgagatttt tgaaagatac tagcaccaag
     2761 cgatcctgtt gcatactggc tgccgcaatg gcggtcgtgt ttttggcatc ttcttttacg
     2821 atggctgcgc aatccaagtc tcttggggtg gtcaccgcta tagaagttat tggcaatagc
     2881 aggattgact ctgatattat tacacaggcc ataaacatta aggtgggtga tgagctcact
     2941 gaaagcgcca taagggaaag cttcaccgct atcgatgctc tcggctggtt tgcggatctc
     3001 ggcgccaaca ccgttccgta ccttggaggc gtgaagctca tcattgtagt gcaagaattt
     3061 ccggttttga gcaagattct agttagcgga aacgcactaa tacctactga ggagcttctc
     3121 ggaataatgg ggtcgaaggt tggctctcag ctcaatgcca atcagctcag gagtgacctg
     3181 gaggctttgg gcagccacta ccatgacaaa ggctactggg tgagcattga accatcactt
     3241 tctgaatcgg gtgaactcat acttcatctg gtggaatgga ctgtgtcgga agtcaagttt
     3301 gcggggaatc agaaaactaa gccgaaagtt atccaaaggt gcattgagac aaagccgggt
     3361 gactacatca atgtcaataa gataaacgag gaccgcaggc gaatctacat gcttggcgcg
     3421 ttcgagaatg tagaggcgaa agtagagcct gtgccgggga agtatgaata cgtggtaacg
     3481 tacgtcctaa ctgaacggaa gacgggcatt gccaatctgg ccctgacata cagttctcag
     3541 gacaagctta tgggttatat ggagctggga gatgagaact tcctcggcaa tgcgcagacg
     3601 gtcaacatca aagcggagtt tggccgcacg aaaaccaatt atgaacttgg cttctatgaa
     3661 ccttgggtcg gagcatccgg caagacttcc ataggcttca acgtcttcag caagactttg
     3721 tcgaagaagt atacgccatc tgatgaaaac gagacttctt cctcagggca agtcttaaag
     3781 ctcgaggagg acccgacggc ctctatcgat tattcgcaga agcggacggg tggagacgtg
     3841 acgatcggcc gcacgctttc gctcaacact aaggcgttcg tcaagctcaa gatggaaaac
     3901 gctgttaatg accctgaaca gaagcatcct gatgtggatt tcccgggcgg cggtgctacg
     3961 cgttcgctta cgttgtcggc ggtcaatgat accagagatg acctttggaa tcctgccaca
     4021 ggtcacaagt tgtccagttc gctggagtat gcaggcggaa ttcttggcgg cgacaacaac
     4081 tttgccaaac ttcgggttga tgcttgccag tactatcagg tccgtgatgg gcatattctg
     4141 gcaggtaggg ttagcgccgg atatggattg gacgagctgc ctgatgatga gcgtttcagg
     4201 ctcgggggag cggagactgt caggggctac aagtacggcg acatcgaggg tgacaggatg
     4261 atcttcgcca acgccgag
//
LOCUS       DAOSAI010000211         4254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13088_length_4254_cov_3.278161, whole genome shotgun sequence.
ACCESSION   DAOSAI010000211 DAOSAI010000000
VERSION     DAOSAI010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13088_length_4254_cov_3.278161"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..481
                     /locus_tag="PLM83_07465"
     CDS             140..481
                     /locus_tag="PLM83_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71278.1"
                     /translation="MDKCEPADQADPVDPVDQGDPEDQANPADQEDPVVPVDQAVPVY
                     PADQVDPADQAVLADMADMANTVSTVDTVDNRDYPHRMAFWVFGTTTYRSPRIEYLIQ
                     CIQQQRHADQD"
     gene            complement(775..850)
                     /locus_tag="PLM83_07470"
     tRNA            complement(775..850)
                     /locus_tag="PLM83_07470"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(815..817),aa:Thr,seq:cgt)
     gene            complement(1040..2200)
                     /gene="pgsW"
                     /locus_tag="PLM83_07475"
     CDS             complement(1040..2200)
                     /gene="pgsW"
                     /locus_tag="PLM83_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOK71279.1"
                     /translation="MKKGCMAPTGRQYGRDRVNFGILLLASLVALAALAVSVRPDRIG
                     LPGDPRGSVGACVEEQIAAAEIMAECIAAVRDARAELGIAIDPDLDPNGTGLIGHDLT
                     PLTTTVGVLEAKRTATSPDFAALMVKYFNEAGLREGDVVAVGASGSFPSLIIATLAAS
                     RAMNLEPVLIYSIGASMYGATIPEFTFIEMLDAIRANGILPYYIAAVSLGGDDDAGGG
                     GLFEESRDISEEIAERSGAYVIREDSIEKSVARRMQVFEEAAAEHGRPIACFVNVGGA
                     SANYGATSASLDFPNGLVMVPKVMSVDPKRGLMFEYAAMGLPVINLIDIRGLAIQNGI
                     PVDPIPLPGPGEGRVYAAHSCSKPAAAVGLAGCLFLIGGAWAKSRRRLQPDV"
     gene            complement(2364..2795)
                     /gene="pgsC"
                     /locus_tag="PLM83_07480"
     CDS             complement(2364..2795)
                     /gene="pgsC"
                     /locus_tag="PLM83_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HOK71280.1"
                     /translation="MTVTVSIGIGLIVGFIFTELTGLYAGGLVVPGYLALYLDQPLRI
                     LATFLVALLAYGIVWVISNFLAIYGRRRFMACVIVGFWLGWLLNRTGMSITPGGPQLR
                     AIGYIIPGLIATDIARQGAARTFLSVLVVAVVVRLLMIVFA"
     gene            complement(2792..3979)
                     /gene="pgsB"
                     /locus_tag="PLM83_07485"
     CDS             complement(2792..3979)
                     /gene="pgsB"
                     /locus_tag="PLM83_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HOK71281.1"
                     /translation="MHGFAVMCACLFVAGVIENWLHTRRLSRIPVRIHVNGTRGKSTI
                     TRLIAAGLRAGGMRVIAKTTGTAARIIFEDGSEEPVVRRGNCANISEQMRLVKLAESR
                     GADAIVVECMALQPENQWAAERHMIKSTVGVITNVRDDHLDVMGPTVHDVAEALSLTV
                     PYGGALVTAEQKFLHVFEDRARKTGASVYVVDASGAPADATANFDYISFPENIACALK
                     VCELCGVPRDLALEGMLTAQGDPGATRILRIDRGKASYVFVNAFAANDRDSTELVCKA
                     AEGRIAPFIRKGLPAIVILNNRSDRPFRAEELAGFVAESISPQWAFVVGEAGRVAVRG
                     LKRGKVAESVIVDLTHQPKAERILAKVADRCPAGAVIYGIGNTRGMGERIIQYFEANG
                     VAL"
BASE COUNT          956 a         1270 c         1089 g          939 t
ORIGIN      
        1 ggtgttccaa ggtttgcaga aaagcccaga gtatgcacgt tcttggtttc ttctccaaca
       61 tttcccgcaa tgttccagcc agatggctca ttagcactat cccaatcgtc aaacgatccg
      121 ttggccacta gattgccaca tggacaaatg cgagccggcg gaccaggcgg accctgtgga
      181 ccctgtggac cagggggacc ctgaggacca ggcgaaccct gcggaccagg aggaccctgt
      241 ggtccctgtg gaccaggcgg tccctgtgta ccctgcggac caggtggacc ctgcggacca
      301 ggcggtcctg gcggacatgg cggacatggc aaacacggtg agcacggtgg acacggtgga
      361 taacagagac tatcctcacc ggatggcgtt ttgggttttt ggaacaacca cataccgttc
      421 acctcgtata gaatacctga ttcaatgtat tcagcagcaa aggcatgcgg accaggactg
      481 aggcacttga caaaggctac cgaaaccgca gagcggactt aacgtgtcgg caaaggcggt
      541 gccagggtga tggagaagta ggcttgcgca tgtattatcc gccatcgcac cattcttccc
      601 ggagcaaagc ggcgagatta ttcctgtctc gttcaccatc ggcacatgcc tctgtcagtc
      661 gtagggagtt ccggaaagac gacaaaaaga aaacgcccct gaatgggcgt ccattttctg
      721 agatctcctg ttgcgtcttg catgattgat tagcacgttc gtgtggtatg ttagtggagc
      781 cggcgaaggg aatcgaaccc ttgacctgcg cattacgagt gcgccgctct gccgactgag
      841 ctacgccggc caatcgaact tcagcaggaa caatgttatc agcttctttc ctgcatgtca
      901 acgggttccg atgtggtatt gggcgtgcta cgattgtggc acgcagggag cgaagcagcc
      961 cagagatctg ggcgaggtgt ctaggttcta ggcacagccc gcgaaagtaa actgaaggct
     1021 gcctataccg agtgcgtccc tagacatcgg gttgcaaacg ccttctgctc tttgcccatg
     1081 ctccgccgat caagaagaga cagcccgcaa gcccgacggc ggctgcgggt ttggagcagc
     1141 tatgagcggc atacacacgt ccctcgccag gacctggaag gggaatgggg tctactggaa
     1201 taccattttg gatggcaagt cccctgatgt ctatgaggtt tattaccggc agccccattg
     1261 cggcatactc gaacatcaag ccccttttgg gatctacaga cattactttc ggaaccatca
     1321 ccaaaccatt gggaaagtcg agtgaagctg aggttgcccc gtagttggcc gaagccccac
     1381 ctacgtttac aaaacaggct atgggccgac catgctcggc tgcagcctct tcgaaaacct
     1441 gcatgcgcct ggctacgctc ttttcgatcg aatcctcacg tattacgtac gctcccgagc
     1501 gctcggcgat ctcctccgat atgtctctcg attcctcgaa caggccgcct ccgcctgcat
     1561 cgtcgtctcc gccaagcgac acagcggcga tgtaataagg gaggatcccg ttggcccgta
     1621 tcgcgtccaa catctcgatg aaagtaaact ctggtatggt ggcgccgtac atcgaagcac
     1681 ctatggagta tatgaggaca ggttcgagat tcatagccct tgaagcagcc aacgtggcga
     1741 taatcaagga cgggaatgaa ccgctggcgc ccactgctac cacatcgccc tcccgcagcc
     1801 cggcctcgtt gaaatacttc accatgaggg ccgcgaaatc gggactggtg gcagtgcgct
     1861 tcgcctccaa aaccccaaca gtagtggtga gcggagtgag atcatgaccg atcagccctg
     1921 ttccgttggg gtcgaggtca ggatcgatag caatgcccag ctcggccctg gcatcgcgca
     1981 cagctgcgat gcattctgcc atgatttcgg cagctgcaat ctgttcctcc acacatgccc
     2041 ctacactgcc tcggggatcg ccaggcagtc caatgcggtc aggccgcacc gaaaccgcca
     2101 aggctgccaa cgccacaaga gacgccaaga gcaagatgcc gaagttgact cgatcccttc
     2161 catattgtcg ccccgtgggc gccatgcaac cctttttcac ccgttcgttc cctccctcta
     2221 acaccctcag gcagccgcca acccccaaga gcgccacttc tttgcgctgc cagcagttcg
     2281 gcccttagac gctttacaat gtcaaatgct tcttacaact tgccacgaaa gcctgtcggc
     2341 aataggatac gtcaacacgc ccttcaggca aaaacgatca taagcagcct aaccactaca
     2401 gcaaccacaa gcactgacag gaatgtgcgt gcggctccct gcctggcaat gtcggttgct
     2461 atgaggcctg gaatgatgta accaatcgcc cgcaattggg gccctcccgg tgtaatgctc
     2521 atgccagtcc tgttcagcag ccagcccagc cagaaaccga ctatgacgca ggccatgaaa
     2581 cgccgccttc catagattgc aaggaaattc gatatgaccc acacaatccc gtaagctagg
     2641 agggccacca ggaatgtggc caatattctg agcggctgat caaggtataa tgcgagatac
     2701 ccggggacca ccaggcctcc ggcgtatagc cctgtcagtt cggtgaagat gaagccgact
     2761 attagtccaa ttccaattga gacggttacg gtcacaatgc caccccgttt gcctcgaaat
     2821 actggattat cctctcgccc atgcccctgg tgtttccaat cccatatatg acggcccctg
     2881 cagggcatcg atctgccact tttgccaata ttctctccgc tttgggctgg tgcgtgagat
     2941 ccacgatgac ggattctgca actttgcccc tcttaagtcc ccgtacggcc accctgcctg
     3001 cctcgcccac cacaaatgcc cattggggtg aaatactctc tgcgacgaag ccggcaagct
     3061 cttcagcgcg aaatggcctg tcagacctgt tgttcaaaat gacgatggct ggcagcccct
     3121 tccggatgaa cggggcgatc ctgccttccg ccgccttgca taccagttct gtagagtcac
     3181 ggtcattagc ggcaaacgca ttgacgaaaa cgtaagaggc cttgccccga tcgatcctga
     3241 gtattcgcgt ggctccagga tctccctggg cggtcagcat tccttccagc gccagatcac
     3301 gcggcacgcc gcacaattca cacactttca gcgcacatgc aatgttttcc ggaaaactga
     3361 tgtagtcgaa gtttgcggtt gcatcagccg gtgcaccaga tgcgtctacc acgtaaaccg
     3421 acgctcccgt cttccgcgct ctgtcttcaa agacatggag gaacttctgc tccgcagtga
     3481 ccagcgcgcc tccgtatgga acggttaacg aaagggcttc ggcaacgtcg tgcacggtcg
     3541 ggcccatgac atccaagtga tcgtcgcgca cgttggttat cacgcccact gtagacttga
     3601 tcatgtgccg ctccgctgcc cattggtttt caggctgaag tgccatgcac tccacgacta
     3661 tcgcgtctgc ccccctcgat tcggccaact tgactagtct catctgctcg gagatgttgg
     3721 cgcaatttcc gcgtcgtaca accggctctt ccgagccgtc ttcaaatatg attcttgccg
     3781 ccgtgcctgt ggtctttgca atcacgcgca tacctccggc acgcaaacca gcagctatca
     3841 acctcgtgat tgtcgatttt cccctagtgc cgttcacgtg tattctaacg ggtattctgc
     3901 tgagacggcg cgtgtgcaac cagttctcga taaccccggc cacgaaaaga caggcgcaca
     3961 tcaccgcaaa accgtgcata catatcaccc ttttgtatcg atgcaggctc gatattcttt
     4021 caattttcga taagcgaggc gcaattcctt ctagcgaaat gtggggtcat cgcccaagaa
     4081 ctttctcatg cagctcagtc agtaaggcaa atgttggctt acaacatata ctcatctcag
     4141 tgcctgtatt tcatctaagt gattcccata agtaactccc aatgagtttt ttgcgctatc
     4201 ggttagcctg ccatatgatg tctgctagga gtagacacgt ctttgcacct atgt
//
LOCUS       DAOSAI010000212         4233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13183_length_4233_cov_3.799426, whole genome shotgun sequence.
ACCESSION   DAOSAI010000212 DAOSAI010000000
VERSION     DAOSAI010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13183_length_4233_cov_3.799426"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLM83_07490"
     CDS             complement(<1..402)
                     /locus_tag="PLM83_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71282.1"
                     /translation="MSEMTECMGDEVVGGEIKVKLARARKYMCERGLDVMILRRFDNF
                     AWITAGGDNRCAGATDIGVASVLITHDDQWVLTSNVEARRLCEEALAHIWAEGIFRMR
                     EHPWYGEAETLSEAAKGFVDGTSLLSYGGRVG"
     gene            complement(399..1220)
                     /locus_tag="PLM83_07495"
     CDS             complement(399..1220)
                     /locus_tag="PLM83_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK71283.1"
                     /translation="MKPKGASKILVQVLAVLLAIAYLIPVYMTVVTSLKEPEEINLMT
                     AWELPSKPNWESYSTAFKQFAPYLKNSFILAISATLISAVMGSLNGYVLCKYPIPGTN
                     IIMPLIVFGMFIPYQSILVPLFQFMQKTKLYGGLPGLILVHVVYGLPITTLLFRSFYE
                     EIPDELLGAASIDGADFFGIFRYLIMPLSGPPFLVVCIWQFTQIWNEFLFAVTLARPD
                     AQPITVALANLAGGQAVSWNLPMAGSILAALPTVLIYVLLGRYFVRGLLAGALKG"
     gene            complement(1227..2339)
                     /locus_tag="PLM83_07500"
     CDS             complement(1227..2339)
                     /locus_tag="PLM83_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK71284.1"
                     /translation="MQSRRDQLTAALIILPSIVLLAVFVYGFIAQTVRVSMTDWGQEA
                     AMSLDPEIHHVGLANYRELFGGFLNMRFRQDITNMFFFTLLFVSLSLLVGLLLATLID
                     QLIWGEAFFRTVFLYPMSLSLVVTGTIWRWLLQPRGGINVLPTLIGLPAGDFLWLSSR
                     EQILIFDWSHIFHYICIAAAVVLAVFAYGHWRRGERKELRAKSIICAVLIGISASGIL
                     TRVKLLDYPEAHGFNRALWGVIVAAVWQMSGYTMALYLAGLRTIPDELREAARVDGAS
                     KFQIYRHVDLPLLKPITVSAIVILGHIALKIFDLIFSMAGPDHVPTSVPAITMFLKTF
                     RGNELAVGSAIGVILLILVSLLIIPYLVNSFKTGKE"
     gene            complement(2427..>2838)
                     /locus_tag="PLM83_07505"
     CDS             complement(2427..>2838)
                     /locus_tag="PLM83_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015717367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOK71285.1"
                     /translation="KPGTGFGWAASPGTKGIFMMLSDSFGLPVGCKNKENAIAWLKML
                     GSRDGQDTFNPLKGSISARTDSDLTKYDAYLQSAARDWRTNKIVGSLAHGVVANERFM
                     NDFATVMDIFLSTKNAQAAANAAQAVAIQAGIGK"
     assembly_gap    2839..2848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2849..3652)
                     /locus_tag="PLM83_07510"
     CDS             complement(<2849..3652)
                     /locus_tag="PLM83_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK71286.1"
                     /translation="MCAAIVLMFSSAADAAVRGKLEIFSWWAGDEGPALEALISKYEK
                     MYPGATVINSTVTGGSGVNAKAVLKTRMLGGDPPDTFQVHAGQELIGTWVVANRMEDL
                     TWLYEQEGWNKVFPADLLNLLSTDKGIWSVPVNIHRSNVLYFVPGNLAKWGVGVPNTW
                     DEFLEIAPKLKAKGVIPLSVGENWTATHLWESVALSVLGPVKWEQLWNGKLSWRSPEV
                     IRVWELFGEILKYTNTDAPSLSWQQATDMVVKGEAAFNIMGDWASGYMTT"
     gene            complement(3798..>4233)
                     /locus_tag="PLM83_07515"
     CDS             complement(3798..>4233)
                     /locus_tag="PLM83_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOK71287.1"
                     /translation="IRTLRDIDIALFDEIKGILPSIVARRCEHVIAENERVQGGVEAL
                     RRGDLRAFGDLMWQSHESLRDLYGVSCPELDMAVEIARKTDGVLGARMTGAGFGGCTV
                     SLVHLEALPHLMEALPDAPMRYGAVQPKTFVVDVVDGARIEE"
BASE COUNT         1004 a         1283 c         1035 g          901 t
ORIGIN      
        1 cccgacacgg ccaccgtaac tgagcagact ggtcccatct acaaagccct ttgcggcctc
       61 acttagggtt tccgcctcgc cgtaccacgg atgctcacgc atgcggaaaa tgccttctgc
      121 ccaaatatgg gcgagcgctt cctcgcacaa tcgccttgct tcaacgttcg acgtcaacac
      181 ccattggtcg tcatgggtga tgaggacaga cgctaccccg atgtccgttg ctcccgcaca
      241 cctgttgtct cccccagcgg ttatccacgc gaaattgtcg aagcgccgca aaatcatcac
      301 atcgagtccg cgttcgcaca tgtacttcct ggctcgagca agtttgacct ttatttcacc
      361 gcccacgacc tcatccccca tgcactcagt catctcgctc atcccttcaa ggctcccgca
      421 agcaatcctc ggacgaaata tcggccgagc agaacataga tgagcaccgt tggaagcgca
      481 gcaaggatcg atccagccat aggcaggttc cacgacacag cctgaccgcc agctagattc
      541 gccagagcca ccgttatggg ctgtgcatcc gggcgcgcca aggtcacagc gaatagaaac
      601 tcgttccata tctgcgtaaa ctgccagatg caaacgacga gaaacggcgg gccagagagc
      661 ggcattatca agtatctgaa gatcccgaag aagtccgccc catctatgct ggctgcgccc
      721 agcagttcgt cagggatctc ttcgtagaag ctcctaaaaa gcagggtagt aatgggaaga
      781 ccatagacta catgcactag tatcaagcct ggtagccccc cgtacagttt cgtcttctgc
      841 atgaactgaa agaggggcac aagtatgctc tgataaggaa tgaacatgcc gaacactatc
      901 agcggcatta tgatattggt ccccggaatc ggatacttgc atagcacata accgttgaga
      961 gaacccatga cagctgaaat cagcgttgca gaaatggcga ggatgaaact gtttttgaga
     1021 taaggggcga actgcttaaa tgctgtggag tagctctccc agttgggctt cgacgggagc
     1081 tcccatgccg tcatcaagtt tatctcctca ggctctttca ggcttgtgac taccgtcatg
     1141 tagacgggga tcagataagc aatcgccaga agaaccgcga gaacctgcac tagtattttc
     1201 gacgctccct tgggtttcat cgcttatcac tcctttcccg tcttgaatga attgaccaga
     1261 taaggaatga tcagcaagga aacgagaatc aacaggatca ctccgatagc ggaaccgaca
     1321 gcaagctcgt tcccgcggaa agtcttaagg aacatggtta tcgcaggcac agacgtcggc
     1381 acatgatcgg ggcctgccat ggagaagatc aaatcgaaaa tcttcagggc tatgtgtcct
     1441 agaatgacta tggcgcttac ggtgatcggc ttgagcagcg gcaaatccac gtgcctgtaa
     1501 atctggaact tgcttgctcc gtcaacccgg gccgcttcgc gaagctcgtc aggtatagtg
     1561 cggagcccgg caagatacaa cgccattgta tagcctgaca tctgccatac agcagcgacg
     1621 ataacacccc aaagcgccct gttaaaaccg tgtgcctcgg gataatccag gagcttgacc
     1681 cgggtcagaa tcccgcttgc cgaaatcccg atgagcacgg cacatatgat ggacttcgcg
     1741 cggagctctt ttcgttctcc acggcgccag tgcccgtaag cgaataccgc cagaacaacc
     1801 gcggccgcta tgcagatgta gtggaaaatg tgggaccaat cgaagataag gatctgctcg
     1861 cggctggaaa gccatagaaa gtcgcctgcg ggcaatccga tcaaggtagg caacacattt
     1921 atgccccctc tcggctggag cagccagcgc cagatcgtcc ccgtaaccac cagcgacaat
     1981 gacatcgggt acaggaaaac cgttcggaag aacgcctcac cccagatgag ctgatcgata
     2041 agcgtagcca aaagcaatcc cacaagcagg gacaggctaa cgaagagcaa tgtgaagaag
     2101 aacatgtttg taatgtcctg cctgaaccgc atgttcaaaa agcccccgaa cagctcgcga
     2161 tagttcgcca gtcccacgtg atggatctca ggatccagcg acatcgccgc ttcctggccc
     2221 caatccgtca tcgacacgcg cacagtctga gctatgaatc cgtacacaaa tactgccaag
     2281 agcactatag acggcaggat aatgagggcc gccgtcaact ggtctcttcg tgattgcatg
     2341 cccttcaccg tccttcttct tgcaggcgca gggcccgtcg ccctcaggcg gcgggccttg
     2401 cgcccgttct atgtagaaca accttcctac ttccctattc cggcctgaat cgccactgcc
     2461 tgggcagcgt ttgctgcagc ctgagcgttc ttcgtgctca ggaagatgtc catgaccgtg
     2521 gcaaagtcat tcatgaacct ctcattggcc acgactccgt gagccaaaga gccgacgatc
     2581 ttattcgtcc tccagtcgcg cgcagcgctt tgcagatatg catcgtattt cgtcagatcc
     2641 gagtcggttc tggcactgat cgagccctta agcgggttga aggtgtcctg cccgtcacgg
     2701 cttccgagca tcttcagcca tgcaatcgca ttctccttgt tcttgcaacc aaccgggagg
     2761 ccaaacgaat cagagagcat catgaatatg cccttggtgc ccggggatgc cgcccaccca
     2821 aacccggttc cgggtttgnn nnnnnnnnag ttgtcatgta tcccgaagcc cagtctccca
     2881 tgatgttgaa cgcggcctca cccttgacca ccatatcagt ggcctgctgc catgagaggg
     2941 atggcgcatc ggtattagtg tacttcaaga tttcgccgaa cagttcccac acccggatga
     3001 cttcagggct tcgccaggag agtttgccgt tccacagctg ctcccatttg acagggccta
     3061 gcaccgatag ggctacactt tcccacaggt gagtggcggt ccagttctcg cccactgaca
     3121 gcggaataac tccttttgcc ttgagctttg gcgcgatctc caagaactca tcccatgtat
     3181 ttgggacgcc aacgccccat ttggcaagat tgccgggaac gaagtacagc acgtttgaac
     3241 ggtggatgtt cacaggcacc gaccatattc ccttgtcggt gctgagcaga ttgagcaagt
     3301 ccgccgggaa taccttgttc cagccctcct gctcatacag ccacgtaaga tcctccatcc
     3361 tgttggccac aacccaagtg ccgatcaatt cctggcccgc gtggacctgg aacgtatccg
     3421 gcggatcgcc gccaagcatc ctggtcttaa gaacggcctt ggcattgact cccgatcccc
     3481 cggtcaccgt ggagttgatg acagtcgctc ccggatacat cttctcatac ttgcttatca
     3541 gcgcttcaag agcagggcct tcgtctccag cccaccatga gaagatctcg agtttcccac
     3601 ggacggccgc atctgcggct gaggaaaaca tcagaacgat cgccgcgcac atcgcagcaa
     3661 gcttgaacca tcttgatgtc ctcattcata tccctccgtc tttaattgat acagctggca
     3721 aatccaacta caccaaacgc cgcattatca tcaataacgc acgtaccctc ccggcaccac
     3781 ctccatgaca gatccagtca ttcctcgatc cttgccccat ccaccacatc cactacgaat
     3841 gttttgggtt gcacggctcc gtaacgcatc ggagcgtcag gaagcgcttc catcaggtga
     3901 ggcaaggctt ccaagtgcac gagactcaca gtgcatcctc caaagcctgc cccggtcatt
     3961 cgcgctccga ggactccatc ggtcttccgg gcaatttcaa ccgccatgtc cagttcggga
     4021 cagctaaccc catacaaatc cctcagactc tcgtgagact gccacatcag atctccgaaa
     4081 gctctcagat caccccgtct tagcgcttcc acgccgcctt gaactctctc gttctcggct
     4141 atcacgtgtt cgcagcggcg ggccactata gaggggagaa tcccctttat ctcgtcaaat
     4201 agggcaatgt cgatatctcg aagggtccgg atg
//
LOCUS       DAOSAI010000213         4230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13199_length_4230_cov_2.903952, whole genome shotgun sequence.
ACCESSION   DAOSAI010000213 DAOSAI010000000
VERSION     DAOSAI010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13199_length_4230_cov_2.903952"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLM83_07520"
     CDS             <1..340
                     /locus_tag="PLM83_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71288.1"
                     /translation="YLADLFGVSFVGWQGVAPLHSIIMPTSDGGVLESPIEVADSRAI
                     IVRRLPGTCELGTWADDDGSPTHADEYNSAVVLSGRVIYVGCDIFAPEMLSDDVRSFV
                     TDALDALFQL"
     gene            450..2816
                     /locus_tag="PLM83_07525"
     CDS             450..2816
                     /locus_tag="PLM83_07525"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOK71289.1"
                     /translation="MPQMQNSRIRIEVDGKTGEVRSIFNIVSRDELVKWPEAVHGAPL
                     RIVCRDLQGHRRVFAPGPASIATATKFNGVDKLALNVEHRTVVASGEAITPCIGSGGE
                     EDDASGLLGCGRGEDEIEIACSWSVELDEENPEISKWALSVDNRSQDYEVIEVLFPYV
                     RGIQVGVAPEDSILIFPHHAGEKIENPASRLASDRYMKFWRAESRIEPDGTYSREINY
                     CGLASMMWMDCYDRNGSGGLYMASYDSDFILTGIRSETGGPDDPWCGFGFRKYVPIGP
                     GEVWSSHPYVLGVHSGDWHWASEQYRSWAEEHLTVTRVPDDLARESAICPRYDFKNGQ
                     TVRNRFVDIPEMYGTAHREHINHFLISGWNRQGFDTDYPEFIPDMELDSSWQLREGCR
                     HVRSEGGFTTFYINVRLFDTESKFFDSLGRAWAAKGLSGEITHEMYGPRTFAVLCPGC
                     GDWQDWAVDTASWMVDAFGARGIYLDQLGSATPLPCYDSGHEHVKVGNRGERPHHGLY
                     NHGYVKMIERIKERCRLIDPSSFLMIENCGDVYSQYLYANLTWNGEEYDEFFNMYKYT
                     FPEFIQVNMVNPRRIDDRDLRYAWFFRDLARAFVLGSVFWMELGDRFGPGNEELREEA
                     LEALRLRQQAAPYIARGTYRDDLGLAFIGECDAAPDPAPRIWWDDEDGSEGLEGSFGH
                     SCDWTCLPFSDLISITASRWTLAGGGVLILMSNPQRLKGKRIWVEDGCGGGAISGSRH
                     HAIQHELGGVRSDLEVVIDDRGGVTLEVPESRLSFIAVLPKEMVIGGR"
     gene            2820..3653
                     /locus_tag="PLM83_07530"
     CDS             2820..3653
                     /locus_tag="PLM83_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOK71290.1"
                     /translation="MNAGERREGSGPVDMHIHTNASDGQPTADEVLKQAKAKGLSMVA
                     ITDHYDPFAAKYGAQSGNVFDDKSLAEQLKWRDGLAGEDDIEVFIGIERGPIPVGTAS
                     LPMQPDFVIGSVHYVTSKIPVERGRLYDERYWRAYMDEVLRVASDPEVDVIGHMAGYL
                     PMGPMLLPGSTFEERRQMEREIDARFFSREWYEDVFRAALANKTAIELHCATCTPRID
                     MVELGLKMGIKFSVGSDAHNVESVGKVAWAYDTLQSVGGGWHNLWCPLPQTRVGARPV
                     R"
     gene            3666..>4230
                     /locus_tag="PLM83_07535"
     CDS             3666..>4230
                     /locus_tag="PLM83_07535"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOK71291.1"
                     /translation="MFEYFDVVRSLLDEVERAEAENMEEAATLIAEAIADGRVLHYFA
                     AGHSHMLGEELFYRAGGLVPVNAIVEPALMVTFGAGKSSHMERLTGLARIILDDAGVG
                     SGDVMIVASNSGVNPVPVEMAAEAKSDGVKVIGLTSVEGSRTTPVRNSVGKRLYEIAD
                     IVIDTHVPWGDAAIEVEGVSQKTGPVST"
BASE COUNT          923 a          990 c         1372 g          945 t
ORIGIN      
        1 ctacctcgct gatctgtttg gcgtttcgtt cgtagggtgg cagggggttg ctcccttgca
       61 cagcatcatc atgccgacaa gtgacggagg cgtactcgag tcgccaatag aggttgccga
      121 ttctcgcgcg atcatagtaa ggcgccttcc aggcacttgc gaattgggca catgggcaga
      181 tgacgatggc agtcctaccc atgctgatga atacaactcc gctgtggtgt tgtcggggcg
      241 cgtgatttat gtaggctgtg atattttcgc gcccgaaatg ctttcagatg atgtgagatc
      301 atttgtgaca gacgcattgg atgcactttt ccagctctaa ctcgtgctgt tgctgtacgg
      361 accatcagtg caagagcttt cggaaatcgt gcaacacaat atgaagggtt ggggacggcg
      421 cagggcgccg aggaacaggg ggaatgtgca tgccccagat gcaaaacagc agaatccgca
      481 ttgaagtaga tggaaagacc ggagaagtca gaagcatatt caatatcgta tctcgtgacg
      541 agttggtgaa gtggcctgag gccgtgcacg gcgctcccct tcggatcgta tgccgcgatt
      601 tgcaagggca caggcgtgta tttgctccag ggcccgcgtc aattgccact gcaacaaagt
      661 ttaacggtgt ggacaagttg gccttgaacg tggagcatcg aacggtggtg gccagcggtg
      721 aggccataac gccgtgtatt ggatctggtg gcgaggagga tgacgcgtcg ggtctcctcg
      781 ggtgcggtag aggcgaagac gagatcgaga ttgcgtgttc gtggagcgtt gaactggatg
      841 aagagaatcc ggagatttcg aagtgggcat tgtccgttga taaccgctca caagattacg
      901 aggtcataga ggttctcttt ccgtatgtcc gcggaatcca ggtaggcgta gcaccggagg
      961 acagcatcct cattttcccg caccatgctg gcgagaagat tgagaacccg gcaagtcggc
     1021 ttgcatccga tagatatatg aagttttggc gagcagagag ccgtattgag cctgacggga
     1081 cttattcccg agagatcaac tactgtggac ttgcgtcaat gatgtggatg gattgttacg
     1141 atcggaatgg ttcgggcgga ctatacatgg cctcatacga cagtgacttc atactgaccg
     1201 ggatcaggag cgagaccggg gggccagatg atccctggtg tggatttggg ttcagaaaat
     1261 acgtgcccat tgggccggga gaggtgtgga gctcgcaccc atacgttctt ggggttcatt
     1321 caggcgattg gcactgggct tctgagcaat acagatcatg ggctgaggag catttgactg
     1381 tgactcgtgt ccccgacgac ttggcgcgcg agtcggcaat ctgtccacgc tacgatttca
     1441 agaacggcca gaccgtgagg aacagatttg ttgacattcc ggagatgtat gggactgctc
     1501 accgtgagca tatcaaccat tttctcatat ccggctggaa caggcagggg tttgacaccg
     1561 attatccgga gttcatcccc gacatggagc tggatagctc gtggcagctg agagagggct
     1621 gcaggcacgt gaggagcgag ggcggattca ccaccttcta catcaacgtt cgccttttcg
     1681 atactgagtc caagttcttc gattccctgg gaagagcgtg ggccgcaaag ggattgagcg
     1741 gcgagatcac ccatgagatg tatgggcctc ggacatttgc agtcctttgt cccggctgtg
     1801 gcgactggca ggattgggcg gtcgacactg catcgtggat ggtcgacgcc ttcggggcaa
     1861 gaggcatcta cctcgatcaa ctggggtcgg ctactccgtt gccatgctac gattcgggcc
     1921 acgagcacgt gaaggtgggg aatcgcgggg aacggccgca tcatggcctc tataaccacg
     1981 ggtacgtgaa gatgatagaa aggatcaagg agagatgccg cctgatagat ccatcatcct
     2041 ttctgatgat tgagaattgc ggcgatgttt atagccagta cctatatgca aacctgacct
     2101 ggaatggcga ggaatacgat gagttcttca acatgtacaa gtacacattc cccgagttta
     2161 tccaggtaaa catggtaaat cccagacgaa tcgacgaccg cgaccttcgt tatgcctggt
     2221 tcttccggga cttggcccgg gcattcgtgc ttgggtcggt attctggatg gagcttggag
     2281 accgtttcgg gcccggaaac gaagaattgc gcgaagaagc tctggaggcg ctgcgcctgc
     2341 gacaacaggc tgccccctac attgctcgtg gtacttatag ggacgacttg gggctggcct
     2401 ttatcggcga atgcgacgct gcgccagatc cggcgccgag gatttggtgg gatgacgagg
     2461 atggttccga gggtctggaa ggcagttttg ggcatagctg cgattggacg tgcttgccat
     2521 ttagtgatct tatatcgatc actgccagtc gctggactct ggccggggga ggagtcctta
     2581 tcctcatgtc taacccgcag aggttgaagg ggaagcgtat ctgggtggaa gacgggtgcg
     2641 gagggggcgc gatttcggga agcaggcacc atgcaatcca gcacgagctc ggaggtgtgc
     2701 gatccgacct ggaggttgtc atcgatgata ggggaggagt cactctggag gtccccgagt
     2761 cgaggctttc gtttattgca gtgcttccca aagagatggt cataggagga cgataggctt
     2821 tgaatgccgg agaacgccgc gagggaagcg gccctgttga tatgcatatt cacacgaatg
     2881 cgtctgatgg gcagcctaca gcggatgaag tgttgaagca ggctaaggcg aaggggcttt
     2941 ctatggtggc cataactgat cactatgacc ctttcgcggc gaaatacggg gcccaatcag
     3001 gaaatgtgtt cgatgacaaa agcctggcgg agcagctcaa gtggagagac gggcttgccg
     3061 gggaggatga tatcgaggtc ttcatcggga ttgagcgggg gcccattccg gtgggaaccg
     3121 catccctgcc gatgcagccc gatttcgtga tcggaagcgt gcactatgtt acatcaaaga
     3181 tccccgttga gcgtggaagg ctctacgatg agagatactg gcgcgcctac atggatgagg
     3241 tgctgagggt agcttcagat cccgaagtgg acgtaatagg acacatggca ggctacctgc
     3301 ccatggggcc gatgttgctt cccgggtcca cctttgagga gaggcggcag atggagcgcg
     3361 agatcgatgc caggttcttc tcgcgcgagt ggtatgaaga cgtcttcagg gcggccttgg
     3421 ccaacaagac ggcaatcgag ctgcattgtg cgacttgcac ccccaggatc gacatggtcg
     3481 agttggggct gaagatgggg ataaagttct ctgtgggcag cgatgcccat aatgttgaaa
     3541 gcgtcggcaa agtggcgtgg gcatatgata ctcttcagtc tgtcggcggt ggatggcaca
     3601 atctgtggtg tccgcttcct cagacaagag tcggtgcgcg cccagtccgt tagggggtag
     3661 tctaggtgtt cgagtatttt gacgtcgtcc gatctttgct ggacgaggtt gagcgggctg
     3721 aggctgagaa tatggaggag gcagccacgc tcatcgcgga agctattgca gatgggcggg
     3781 tgctccatta tttcgcggcc ggccattctc acatgctcgg agaggagctg ttttacaggg
     3841 cagggggact tgtgcctgtg aacgccatcg tcgagcccgc gctcatggtt actttcggcg
     3901 ccggcaagag ctcccacatg gagcggctta cagggcttgc caggatcata cttgatgatg
     3961 caggagtagg cagcggagac gtcatgatcg ttgcatccaa ttccggagta aaccctgtgc
     4021 cggtggaaat ggcggccgag gccaagtccg acggcgtaaa ggtgataggc ttgacgtctg
     4081 tcgagggttc tcggaccact ccggtcagaa acagcgttgg caagcgactt tatgagatcg
     4141 ccgacatcgt aatcgatacg cacgtgccat ggggcgatgc ggctatagag gtggaagggg
     4201 tttcgcagaa gaccggacct gtttcgacgg
//
LOCUS       DAOSAI010000214         4214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13256_length_4214_cov_3.969464, whole genome shotgun sequence.
ACCESSION   DAOSAI010000214 DAOSAI010000000
VERSION     DAOSAI010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13256_length_4214_cov_3.969464"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..3997
                     /gene="rpoC"
                     /locus_tag="PLM83_07540"
     CDS             173..3997
                     /gene="rpoC"
                     /locus_tag="PLM83_07540"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOK71292.1"
                     /translation="MLDVNDFDRIRIGMASPEQIRAWSSGEVKKPETINYRTLKPERE
                     GLFCEKIFGPTRDWECHCGKYKRIRYKGVVCDKCGVEVTRAKVRRERLGHIELAAPVS
                     HIWYFKGIPSRMGLLLDMSPRSLEKVLYFASYIVTDPGDTPLSKKQLLTESEYREYRE
                     KYGQLFKAGMGAEAIQELLRDIDLDELVQELRDDIENSSGQKRVRAIRRLEVAEAFRK
                     SGNRPEWMILDVIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLLDLEA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSMSDLLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPTLKMHQCGLPKEMALELFKPFVMKRLVDKGYAHNIKSAKRMVER
                     VRPEVWDVLEEIIAEHPVLLNRAPTLHRLGIQAFEPVLVEGRAIQIHPLVCTAYNADF
                     DGDQMAVHVPLSAEAQAESRLLMLSVNNILSPAHGGPLATPTQDMVLGCYYLTFERIG
                     AKGEGKLFENPEAARAAYDAGAIALHSMIVVRIPESKLLLGAEEAGTRRIVTTVGKLI
                     FNEIFPDDFPYINSAESSFDSALTEGDFLPRGVSFWEYEESGGNRMRKPATKGFLSKL
                     VGVIRRKYGNARTADVLDALKNLGFHYATVSGTTVGIVDIAVPDKKQAIIKDADARVE
                     EIEGQYRRGLLLEDEKYSLIIDVWTEARENVQEAMMREIRPDILMARKLEEAKLTGER
                     ERVEFKIDELNPVYMMATSGARGNVQQLNQLAGMRGLVADPSGRTIDVPIKANFREGL
                     NVLEYFISTHGTRKGLADTALRTADSGYLTRRLVDVAQDVIVREDDCGTTEGIDVSAI
                     VEGDTVIESLSERITGRVSLQELVNEETGEVIVAKNQMIDEDQAKMIEDLGYESWAVR
                     SVLKCRTRHGVCVMCYGRNLATGNLVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVA
                     GDDITRGLPRVEELFEARKPKGLAIITEYAGEVSMVEAKGSRKIVITQPSGESHEYSV
                     PYGARLEVKEGQKVEPGQKLTEGSLNPHDILRIRGSVAVQQYIVREVQDVYRSQGVEI
                     NDKHIEVVVRQMMRKVKVENPGDTDLLPGELVDIFRFEDTNAEAKANGLRPATCKPAL
                     LGITKASLQTDSFLSAASFQETTRVLTEAAMKGKSDELIGLKENVIIGKLVPAGTGMP
                     RYRNVRVLASPEASREIERDIEKAERGRRDVREAASPGAARADSLGAPLAEHLGKEIT
                     EALSEGASQAQS"
BASE COUNT         1003 a          991 c         1342 g          878 t
ORIGIN      
        1 ccacgagggt gggcaaaacc cgtgacgaag atggcgagga tgaacctgat gacagtgatg
       61 atgaagatca gctcgacacc gaggatgaag gagacagcga ctacgatgcc gattaggatg
      121 cgggtggcgg ataacgggtc ggagccccag ggcgaaggag ggggaggggc ggatgctgga
      181 cgtcaatgat tttgatcgaa ttagaatagg aatggcatcg ccagagcaaa tccgggcctg
      241 gtcgagtggt gaggttaaga aacctgagac gattaactat cgtactctga agcctgaacg
      301 cgagggactt ttctgtgaga agatctttgg cccgacgcgt gactgggaat gccattgcgg
      361 caaatacaag cgcataagat acaaaggcgt cgtatgtgac aagtgcggcg tggaggttac
      421 ccgggcaaag gttcgtcgcg agaggttggg ccatattgaa ttggctgccc cggtctcgca
      481 tatttggtat ttcaaaggaa ttccgagccg aatgggcctc ttgctggata tgtctcctag
      541 gtcattggag aaagtgcttt actttgcatc ctacatcgtg actgatccgg gagatacgcc
      601 gctttcgaag aaacagctat taactgaaag cgaataccgt gaatacagag agaaatacgg
      661 acagctcttc aaggcgggaa tgggagccga ggcgatacag gagcttctcc gcgacatcga
      721 tttggatgag cttgtccaag agcttcgcga cgatattgaa aacagttctg gccagaaacg
      781 tgtgcgtgcc atacgcaggt tggaggttgc tgaagctttt cgaaaatccg gaaaccggcc
      841 tgaatggatg atactcgatg tcattcctgt gatacctccg gatctgcgcc caatggtgca
      901 gttggacggt ggcaggttcg ccaccagcga cttgaatgat ctgtatcgtc gggtcataaa
      961 caggaacaac aggctgaaga agcttctgga cttggaggcg cccgacatta tcgttcgcaa
     1021 cgaaaaaagg atgctgcaag aggcggtgga cgctcttatc gacaatggtc gcaggggaag
     1081 gccggttaca ggacctggca acaggccctt gaagtcgatg tcagacttgc tcaagggcaa
     1141 gcagggaagg ttcaggcaga accttctcgg aaagcgtgtc gactactcgg gcagatccgt
     1201 tattgtcgtc ggaccgactc tcaagatgca tcagtgcgga ttgcccaagg agatggctct
     1261 tgagttgttc aagccttttg ttatgaagag gcttgtcgac aagggctatg ctcataacat
     1321 aaagagtgcc aagcggatgg tggagcgcgt aaggcccgag gtgtgggacg ttctcgagga
     1381 gattattgct gagcatccgg tgcttctaaa ccgtgctcct acccttcata ggctcggcat
     1441 tcaggcgttt gagcccgtct tggtggaggg cagggccata cagattcatc cgcttgtctg
     1501 caccgcctac aatgcagact tcgacggtga ccagatggcg gtccacgtgc cgttgtccgc
     1561 tgaggcgcag gccgaatcga ggttgctcat gctgtcggtg aacaacattc tttcaccggc
     1621 tcacggcggc ccgctggcta cgcccacgca agacatggtt ttgggctgct actacctgac
     1681 attcgagcgg ataggcgcca agggcgaggg gaagctgttt gagaatccgg aagcggctcg
     1741 agcggcttat gatgctggcg caatcgcatt gcattccatg attgtagtgc gaattccaga
     1801 gtccaagctt ctgttgggag cggaggaggc cggcactcgc aggatagtga ctaccgttgg
     1861 caagcttata ttcaatgaga tattcccgga tgacttcccc tatataaact ccgcggagtc
     1921 atcatttgat tcagcgctca ccgagggtga cttcttgccg aggggtgtct ctttttggga
     1981 gtatgaggag tcaggcggca atcggatgcg caaaccggct accaagggtt tcttgagcaa
     2041 actcgtgggt gtgatcagga gaaagtacgg gaatgcgagg accgctgatg tattggatgc
     2101 cctgaagaac ctcggatttc attacgccac tgtgtcaggg actacggtag gcatagtcga
     2161 cattgctgtg cccgacaaga aacaggccat cataaaggat gccgacgcca gggtcgagga
     2221 gatcgagggt cagtacagaa gaggcttgct tctggaggac gaaaagtaca gcctgataat
     2281 agatgtttgg actgaggcgc gcgagaacgt tcaggaagcc atgatgcggg agattcggcc
     2341 ggatattctg atggcccgca aactcgagga ggccaagctg actggggaga gagagcgtgt
     2401 ggagttcaag atcgacgagc tcaatcccgt atacatgatg gccacatcgg gcgctcgtgg
     2461 taatgtgcag cagctcaacc agcttgcagg gatgagaggc ctcgtcgccg acccgtcagg
     2521 acggactatc gacgtcccca tcaaggccaa cttcagggaa ggcctgaatg tgttggaata
     2581 cttcatttcc acccacggga ctcgtaaggg gctggccgac actgctctta gaacagctga
     2641 ctcaggctac ctcaccaggc ggcttgtgga tgtggctcag gacgtaatcg tcagggagga
     2701 tgattgcggc actaccgagg gaatcgatgt gtccgcgatt gttgaaggcg atacggtgat
     2761 cgagagcctg tcggagagga tcactgggcg cgtgagcctg caggagctgg tgaacgaaga
     2821 gaccggcgag gtgatcgtcg ccaagaacca gatgatcgat gaggaccagg ccaagatgat
     2881 cgaggatttg ggatacgagt cttgggctgt gcggtcggtt ttgaaatgta ggacgcgcca
     2941 tggtgtgtgt gtcatgtgct acgggcgcaa tcttgcaaca ggcaacttgg ttgaagtagg
     3001 cgaagcggtc ggaaccattg ctgcgcaatc cattggcgag cccggcacgc agctgactat
     3061 gaggacattc cacacgggcg gcgttgcagg ggacgacatc acccgcggcc tgcccagggt
     3121 tgaggaactg ttcgaggcga gaaagcccaa gggccttgcc atcatcactg agtatgctgg
     3181 cgaagtaagc atggttgagg caaaggggtc tcgcaagata gtcataactc aaccttcagg
     3241 cgaatcccat gagtactccg tgccatatgg tgcacggctg gaggttaagg aaggccagaa
     3301 ggtcgagccg ggccagaagc tcactgaagg gtcgctcaat ccccacgata tattgcgaat
     3361 tcgcgggtca gtagctgtgc agcagtatat agtcagagaa gtgcaggacg tctaccgttc
     3421 ccagggtgtc gagatcaatg acaagcatat cgaggtagta gtcagacaga tgatgcgcaa
     3481 ggtgaaagta gagaatccgg gagacacgga tctgctcccc ggcgagctgg tagacatctt
     3541 caggtttgag gacactaatg ctgaagccaa ggcgaatggg ctcaggccag ctacgtgcaa
     3601 gccggcgctt ctcgggatca ccaaggcgtc actgcagaca gacagcttcc tgtcggcggc
     3661 ttcgttccag gagactacca gagtccttac ggaagctgct atgaagggca agagtgatga
     3721 gctcattggt ctaaaggaga atgtcatcat aggcaagctt gttccggcag gcaccgggat
     3781 gccgagatac cgcaatgtga gggtccttgc gtctccggaa gccagtaggg aaatcgaacg
     3841 cgatattgag aaggccgaaa gaggtcgacg agatgtacga gaagctgcat ctcctggggc
     3901 cgcgagggca gattctcttg gagcgccctt ggcggagcat ctggggaagg aaataacaga
     3961 ggccctgagc gagggggcgt cgcaggccca gtcctagaca gcccggctat cttacgctct
     4021 cggctaggct aggttgtgtg gccgcatgtt cgcgtgagtt ttgcgtgggc gtgcggtcgc
     4081 ttcttggaac cggcggttct gttggagtaa atctcgcaga cgcgtaacaa gctcgttgtg
     4141 ccccctgtca accgactgtg aaaatgtcac cccctgtggt tgtgttccgc tggcgagccc
     4201 gcgcattatg cgca
//
LOCUS       DAOSAI010000215         4203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13305_length_4203_cov_3.728303, whole genome shotgun sequence.
ACCESSION   DAOSAI010000215 DAOSAI010000000
VERSION     DAOSAI010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13305_length_4203_cov_3.728303"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /gene="mutL"
                     /locus_tag="PLM83_07545"
     CDS             <1..1846
                     /gene="mutL"
                     /locus_tag="PLM83_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOK71293.1"
                     /translation="TSLRPCVRAEGSGQDEGVNWMRIIRLAPEVANKIAAGEVVERPA
                     SVVKELVENSIDAGANTISIETLGGGLELVMVTDDGCGMGREDAARAFERHATSKIAR
                     AEDLFSIESLGFRGEALPSIASVARVRLVTCERGSGEGTVVVAEGGRVGEPTAIGAPC
                     GTSIAVRDLFFNTPARLKYLRSVSTENRRIIGTVGSLALAHPEIGFSLKVDGRTALVT
                     PQGGSIEEAAAAVLGLEVAREMIPVKSSSHGIFVKGLISLPQHAKGKRRDSQYIFVNG
                     RPVVSTTVWSAIDKAYDKTVPVGIRPPVILSIEIDPSQIDVNVHPAKSEVRFASNSDV
                     FSAVCGALAFVLRSVDAVPDLEPVQPTAGWGQTGAVSRQWQGPGASYGGEPQHLAISS
                     WDRESAFREVRPASGPGVALGLAPARSGLAGAVPEVIGQLDDTYILAQGAEGLVIIDQ
                     HVAHERILVDKYREEFAKEKPSVQLLLTPEIAEIGASDVEIVAKHGDVLSRMGYELDL
                     FGATSVVIRGVPSQSAGVPPAESLMAAIEGIVSAPSGSDPGLLADHIAVSLACRGAVK
                     AGDSLTIAEMKSLVERLFESDDPYRCPHGRPVVVLVPGDALARSFGRK"
     gene            2198..3043
                     /locus_tag="PLM83_07550"
     CDS             2198..3043
                     /locus_tag="PLM83_07550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK71294.1"
                     /translation="MKDVGRIAVTTSARGGRHQVDLGIRLAHELGAPFITRAGRSLQS
                     LADAYSEDGRGELEAFMVVEADRISVVRLADAGRRSAELFFHPGMALSRIRRLKQGGV
                     DQMVEAMGISSGDHVLDCTIGLASDAIVSSWVVGSRGRVVGVEVSPVLSIITREGLTS
                     YTEADPDVLDAMRHIEVVASDHLDYLRSCADKSFDVVSFDPMFREPVTSSAGIAPIRP
                     WACHTPLSVEALSEACRVARRRVVVKDRADGDELDKLGVGHKIGAKKSRQEYGVIFVE
                     GERWD"
     gene            3021..4100
                     /gene="miaA"
                     /locus_tag="PLM83_07555"
     CDS             3021..4100
                     /gene="miaA"
                     /locus_tag="PLM83_07555"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOK71295.1"
                     /translation="MKESDGTDRVREAPRGESVGDGIGRVGNGAGHPGERDFQRRPPL
                     LVIVGPTAVGKSAVGMELARKLGGEIVSADSMQVYRGMDIGTAKPSQAERAEIPHHLI
                     DVADPDEIFCVARYQEMAREAIRDIAARGRLPIMVGGTGLYVDAVVRGFLFPDEGRNP
                     DIRRRLEGEAEVTGSPALHVRLAEVDPVAAERIHPNDLRRIVRALEVYEVTGMAISEL
                     QRKHEGESEFDTVIYGLRMARQALNKRIDDRVDIMVEQGLIDEVRSLMEHGYDPRYTA
                     VQAIGYKEFALYLAGEESLEEAIDTLKRETRKYAKRQMTWFRRNPNIRWIDVECGRGP
                     EDIADEIAASIESWLKGVGFSVGSK"
BASE COUNT          924 a         1012 c         1378 g          889 t
ORIGIN      
        1 gacaagcctt agaccttgtg tacgagctga aggatctggc caggatgaag gagtgaactg
       61 gatgcgtata atcaggcttg cccctgaagt cgccaataag atagccgcag gcgaagttgt
      121 tgaaaggccc gcgtcggttg tgaaggagct tgtagaaaac tccatagacg ctggggctaa
      181 caccataagc atagagacgc taggaggcgg tctggagctt gtcatggtta cagacgacgg
      241 ttgcggcatg gggcgagaag atgcggcgcg ggcctttgaa cgccatgcca ccagcaagat
      301 tgcccgggcg gaagacctct tttccataga aagcctcggc ttcaggggcg aggctctccc
      361 gagcatcgct tcagttgcca gagtgaggct cgtcacctgt gagcgcggaa gcggtgaagg
      421 gacggtggta gtggcggaag ggggacgagt gggagagcct acagctatcg gggcaccctg
      481 tggtacaagc attgccgttc gggatctctt ctttaacacg cctgcaaggc tcaagtattt
      541 gcgttccgtc tctacggaga acaggaggat cataggcacc gtgggcagcc tggccctggc
      601 ccatcctgaa atcggattta gcctcaaggt ggacgggcgc acggcgcttg tcactccgca
      661 aggcggatcg attgaggagg cagctgccgc ggtcctcggg ctggaggtag ctcgcgagat
      721 gattccggta aagagttcct cccacgggat cttcgtaaaa ggcctcatct ctttgcctca
      781 gcacgccaag ggcaaacgcc gcgactcgca atacatattt gtaaacggtc gtccggtcgt
      841 atccacgaca gtctggtctg ccatagacaa ggcctacgac aagactgttc ccgtaggcat
      901 cagacctccg gtaattctct cgatcgagat tgatccgtct cagatcgacg tcaacgttca
      961 cccagccaag agtgaagtga gattcgcgtc gaactccgat gtattctccg ctgtctgcgg
     1021 agccctagcc tttgtgctgc gttcagtcga tgccgtccca gacctggagc ctgttcagcc
     1081 cacagcagga tggggccaga cgggtgcagt atctaggcaa tggcaggggc cgggcgcctc
     1141 gtatggaggt gagcctcaac atcttgccat ctcgtcatgg gatcgggaga gcgcgttccg
     1201 tgaggtgagg cctgcgtccg gaccaggcgt tgctcttggg ctggctccgg caagatcagg
     1261 ccttgcaggc gcggtccctg aggttattgg acagctggat gatacgtata ttcttgcgca
     1321 aggtgccgaa ggcttggtca taatagatca gcatgtcgca cacgaacgta ttttggtgga
     1381 caaataccgg gaggaattcg cgaaggagaa gccctctgtc cagctccttc tgacacctga
     1441 gatcgcagag ataggcgcgt cggacgtgga gatcgtggcc aaacacggag acgttctgtc
     1501 gagaatgggt tatgaactcg acctgttcgg tgcgacctct gtcgtgatca gaggagtccc
     1561 ctctcaaagc gccggggtgc cgccggcaga gtcgctcatg gccgccatcg aagggattgt
     1621 ctcggcgcct tcagggtctg atcccggatt gctggcagat catatcgcgg tgtcgcttgc
     1681 atgccgcgga gcggtgaagg caggcgattc tctcactatt gctgaaatga agtctcttgt
     1741 ggagaggttg ttcgaatctg acgatcccta tcggtgtccg catggacggc ccgtggtcgt
     1801 gctagtgccg ggggatgcgt tggcaaggag ttttgggcgc aagtaaaccg ccccacgccg
     1861 aaaggcaggg gattactgat tggtgcactg tggctgatgg ctaggcaaaa gtggtgaata
     1921 gaacacctca cgtttacgag gcctatggat acgtcacacc taaagtatgc tagatctcga
     1981 gaagggtgtt tggagggcca acagggcacc tagcgtgtcc atgggtttca gctatttgac
     2041 aaggttgagt atatgtgctt tgttttcgca agacgtatca caggctattg tgacattcgg
     2101 cacaaggatt tcggcatcgg ccaatgctgt acgaaaggag ggaaaacgct acttcccccc
     2161 atatctgagg gagggggtct ttgtagcgtg acatttgatg aaggacgttg gccgtattgc
     2221 cgtgactaca tccgcgcggg gcggacgcca tcaggttgat cttggaataa gacttgcaca
     2281 cgagcttggt gcgcctttca taacgagggc agggagatcg ttgcaatctc ttgcggacgc
     2341 ttacagcgag gatggtcgtg gcgagctgga agcgttcatg gttgttgagg cggacaggat
     2401 aagcgttgtg aggttggcgg atgcgggcag gcgcagtgcg gagttgttct tccatcccgg
     2461 tatggccttg tcgaggattc gtcgactgaa gcaaggtgga gtagaccaga tggttgaggc
     2521 catgggaata tcctctggcg atcacgtgct cgactgcacg atcggcttgg cgtcagacgc
     2581 tatagtctct tcatgggttg tgggaagccg tggacgggtt gtgggagtgg aggtcagtcc
     2641 ggttctttcc attatcaccc gtgaggggct cacgagctat accgaggcgg atccagacgt
     2701 cctagatgca atgaggcaca tagaggtggt cgcctcagat catctggact atctcaggtc
     2761 atgtgcagac aagagctttg atgtagtctc cttcgatccc atgtttaggg agccagtgac
     2821 gtcatccgca ggtattgccc ccatacggcc ctgggcatgc cacacgccac tttccgtgga
     2881 agcgctttcg gaggcctgca gggtggcacg cagacgtgtc gtcgtcaagg acagggcgga
     2941 cggggatgaa cttgacaagc tgggcgtggg ccataagatc ggggcaaaga agagtcggca
     3001 ggaatacggg gtgatattcg ttgaaggaga gcgatgggac tgaccgggta agggaggcgc
     3061 ctcggggaga gagcgtcggg gatggtatag gccgagtggg aaatggggct gggcatccgg
     3121 gtgaacgtga ctttcaaaga aggccgccgc ttctcgtcat agtcggcccc accgctgtcg
     3181 gcaagtccgc agttggcatg gagcttgcgc gcaagcttgg gggggagata gtgtcagccg
     3241 attccatgca ggtctaccgg ggtatggata ttggcacagc taagccttcc caagcggagc
     3301 gggcggagat cccccatcac cttatagatg tcgctgaccc cgacgagatc ttctgtgtag
     3361 cccggtatca ggagatggcc agggaagcca tacgcgacat agcggctcga gggagactgc
     3421 caatcatggt gggtgggacc gggttatatg tggacgcggt tgtccgcggt ttcctattcc
     3481 ccgatgaagg gcgaaatccc gacatacgcc gccgtcttga gggagaggct gaagtgacag
     3541 ggagccctgc gttgcacgtt cgtcttgccg aggtggaccc tgttgcggca gagcgaatac
     3601 accccaatga cctgaggcga atagtgaggg cgttggaagt ttacgaagtg accgggatgg
     3661 ccatatccga gctccaacgc aaacacgaag gagagagcga gtttgacacg gtcatctacg
     3721 gactgcgcat ggcgcgccag gcgctaaaca agcggataga cgaccgtgtg gacattatgg
     3781 tcgagcaggg cctgatcgac gaggtccgct cgctgatgga acacggctat gatccgagat
     3841 acactgcagt gcaggctata ggatacaagg agtttgcctt gtaccttgcc ggggaggaga
     3901 gcctcgaaga ggcgatcgac acattgaagc gggaaacgag gaaatatgcg aagaggcaga
     3961 tgacatggtt ccgcaggaat cccaacatac gttggatcga tgtggagtgt ggcagagggc
     4021 ctgaagatat tgcagatgaa atcgcagcgt ctattgagag ctggctcaaa ggagtgggat
     4081 tttctgttgg atcgaagtga agtggcccaa attctcgggg tcgatgacga tatcatggaa
     4141 ttggcttcct gcgtacacga agaaatatcg gacgagatag cgacagtaaa ttcgatatgc
     4201 aga
//
LOCUS       DAOSAI010000216         4182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13391_length_4182_cov_4.669736, whole genome shotgun sequence.
ACCESSION   DAOSAI010000216 DAOSAI010000000
VERSION     DAOSAI010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13391_length_4182_cov_4.669736"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2769
                     /locus_tag="PLM83_07560"
     CDS             <1..>2769
                     /locus_tag="PLM83_07560"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOK71296.1"
                     /translation="NGIYGLDPETGAVVMKITDGPWTYSSQRGLAYRAEDDTFFIGGW
                     DEDIIYHINGPSHESPGSVIGAYKFPVGIAGLAWHPDGILWVSNNGSPDMIFGLDLDA
                     LEVVHQFPHPFGGEYHGGGLALNSDGNLWVVSIDNSRVYLVSTEMPWAPGIAMSSIDG
                     TVAMGETEELDVTINAEALGKPGDDVRRCLRIRTNDPENAILYVDLIVHIEAGPTIAE
                     ATATPTIGEPPLKVAFNAVTEPGATDIIDTWWDFGDGSDLVHEAATEHVYTDVGAYKA
                     TFHAIDENEVEAIAELAVTVKWLPVLGVEPGEINAIIQAGEEEQAVLTVSNTGVAPMN
                     FEISIVPDLPKDPNGEPAGCGYIWMDSNHDGGPAFDWFEISDVGTGVKLYDESFVDVD
                     LPFPFPFYGEMKTEVRICSNGYLTFGMTRSEWTNTPIPDSSDPNDLIAIFWDDLNPGS
                     GGNVYYYHDQEAGSFIVEYQAVPGRTDSGDYTFQAILYPDGTIIFQYLEMTADVTGAT
                     VGIENATGTDGLQVVYNAPYIEDGLAIIFAPIESILRVNPSSGYIVPGGSQEVQLTLG
                     SPDAAYGTYSLRLCVAADNPLYPFATVPVTIKVNAAPSVAITAPVGGDELHGKYEITW
                     VATDHDDDEDELLIDLAWTRDGETWHELGTGLANTGSFEWNTTLVGEAGDAFRLRARV
                     TDPAGAFHEFVTDEFTIVNLAPNADFRFTPSPATRRDKVKFVDESTDDGEIVAWHWEF
                     GDGAESEQESPEHQYTQKGEFEITLIVTDNGGLTGSATKTIQVGNAKPIAAFSFTPSP
                     ARVGEVVRFTNESTDDGDIVACFWEFGDGATSAQCSPEYVYSSTGTFAVKLTVIDDDN
                     VASSITREIEIINLPPEVEIVRPVAGQVLTGEAMIEWEAFDPDDDADTLKITLEYKPA
                     SGGDWQAI"
     assembly_gap    2770..2869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2870..3328
                     /locus_tag="PLM83_07565"
     CDS             <2870..3328
                     /locus_tag="PLM83_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK71297.1"
                     /translation="LKITLEYKPASGGDWQAIASEVPNTGKYVWDTSKLERGGKYEIR
                     ITAVDPEGASGEAISEEFTVIALTRTVVAAPNPTSDVVTFYYDIAANGTLYVYDIAGR
                     LVHSAELSATANAHEWNLHSSGKPVANGVYLYVVIAGEERSEVGRLVVSR"
     gene            3404..>4182
                     /locus_tag="PLM83_07570"
     CDS             3404..>4182
                     /locus_tag="PLM83_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71298.1"
                     /translation="MRIKKTVLALVLVIALIPASAAFAEDDPVGTAAMMGLGMGARAL
                     GMGGAHIAVADDASVIYYNPAGLGVVDGISVTSLYTNQYGAAGYLALGGVARHIGAGI
                     LRLDASGIEETDEFANEIGTFGVASTAVMAGYGMTVMPNLSVGAAVKYYQETLPENSG
                     KGFTADMGVLYSMADDKIRVGAVGRNLFGKVAYSSGAEDAFDRAFGLGVAFEPMENLL
                     VAADAVMDNGFSGKVGAEYRFRQMALRAGGSFGGNGTCLTAG"
BASE COUNT          964 a         1080 c         1252 g          786 t
ORIGIN      
        1 acaacggcat atacggactt gaccctgaaa caggcgcagt ggtcatgaag atcacggatg
       61 ggccttggac atactcatcg caacggggcc ttgcttatag ggcggaagat gataccttct
      121 tcatcggcgg atgggacgag gacatcatct accatatcaa tgggccaagc cacgagagcc
      181 ccggctcggt gatcggcgcc tataagttcc cagtaggcat agccggactc gcatggcacc
      241 cagacgggat cctgtgggta tcgaacaacg gctctcccga catgatattc gggctcgatc
      301 tcgacgcgct cgaagtcgtg caccagttcc cgcatccatt cggcggagag taccatggag
      361 gtggactcgc gctcaacagc gatggcaacc tgtgggttgt atcgatagat aatagccgcg
      421 tgtacctcgt gagcaccgaa atgccatggg cgccgggaat agccatgtct tcaatagacg
      481 gcacagttgc catgggcgaa accgaagaac tggatgtcac gatcaacgct gaagcgctgg
      541 gcaagccggg cgatgatgtg aggcgttgct tgagaataag gactaacgac cccgaaaacg
      601 ccatactcta cgtggacttg atcgtccaca tcgaggcagg gcctaccatt gccgaagcca
      661 ctgcgacgcc gaccataggc gagccgccgc ttaaggtggc attcaatgca gtgaccgaac
      721 cgggtgctac cgacataatt gatacctggt gggatttcgg cgatggcagc gacctggtgc
      781 atgaagccgc aacagagcac gtatacactg acgtcggagc atacaaagcg accttccacg
      841 caatagatga aaacgaagtc gaggcgattg ccgagcttgc agtcacagtc aaatggctgc
      901 ctgtgcttgg ggttgaacca ggcgagatca atgcgatcat tcaagccggt gaagaagagc
      961 aggctgtctt gacagtgagc aacacaggcg tggcgccgat gaatttcgaa atatcgattg
     1021 tgccggacct ccccaaagat cccaatggcg aacctgccgg gtgtgggtac atctggatgg
     1081 acagcaacca tgacggtggt cccgcgttcg attggttcga gatcagcgac gtcggaaccg
     1141 gggtgaaact gtacgacgag tcctttgtcg atgtggatct tccgttcccc ttcccgttct
     1201 acggggagat gaagactgag gttcgcatct gctccaacgg atacctgacg ttcgggatga
     1261 cgaggagcga atggacgaat actcccatac ctgattcgtc cgaccccaac gacctgattg
     1321 cgatcttctg ggatgacctc aatccgggta gtggcggcaa cgtctattac tatcacgacc
     1381 aggaagcagg cagcttcatc gtggaatacc aggcggtgcc ggggcggact gactctgggg
     1441 actatacatt ccaggcgata ctgtatcctg acggcacgat catattccaa tacctcgaga
     1501 tgaccgctga cgtgacaggc gccacggtgg gtattgagaa cgccacgggc acagatggcc
     1561 tgcaggtggt ctacaacgcc ccctacatcg aggatggctt agcgataatc ttcgcgccga
     1621 tcgagtcgat cctcagggtt aacccgtcgt cggggtacat tgtgcccggc ggaagccagg
     1681 aagtccagct gacgctcgga tcccctgacg cagcgtacgg cacatactcg cttcgcttat
     1741 gtgtagcggc cgataacccg ctctatccgt ttgctaccgt cccagtgacg atcaaggtta
     1801 acgccgcacc tagcgtcgcg atcacggcgc ctgtgggcgg agatgagctg cacgggaaat
     1861 acgagatcac atgggtggcg acggaccatg atgatgacga agacgagctc ctgatcgatc
     1921 tggcatggac tcgcgatggc gagacgtggc atgagctcgg gacagggctt gccaacaccg
     1981 gctccttcga atggaacacc accctagtgg gcgaggctgg cgatgccttc cggttgaggg
     2041 caagagtcac cgacccggca ggcgcgttcc acgagttcgt gacggacgag ttcaccatcg
     2101 taaacctcgc cccgaacgcc gacttcagat tcaccccgtc ccccgccaca cggcgcgaca
     2161 aggttaagtt cgtcgacgag tcgacagatg acggcgagat agtggcgtgg cattgggagt
     2221 tcggggatgg cgcggaaagc gaacaggaga gcccagagca ccaatacacc cagaagggcg
     2281 agttcgagat aacgctcatt gtcaccgaca acggcgggct gacaggaagt gcgacaaaga
     2341 ccattcaggt ggggaatgcg aagccgatcg cggcattcag tttcacccca tcgccagcaa
     2401 gagttggcga agttgtgagg tttaccaacg aatctactga tgatggtgat atagtcgcat
     2461 gtttctggga gttcggcgat ggggccacga gtgcccagtg cagccctgag tacgtataca
     2521 gctctacagg cacgttcgcg gttaagctga cagtgataga tgacgacaat gtagctagct
     2581 cgataacgcg cgaaatagag atcatcaacc ttccgccgga agtcgagatc gtaaggcctg
     2641 tggcaggcca ggtcttaacg ggcgaggcga tgatcgagtg ggaggccttc gatcccgatg
     2701 acgatgcaga tacgctcaag atcactcttg aatacaagcc tgcatcaggc ggggattggc
     2761 aggccatagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tcaagatcac
     2881 tcttgaatac aagcctgcat caggcgggga ttggcaggcc atagcatctg aggtgcccaa
     2941 caccggtaaa tacgtctggg atacctccaa gctggagcgc ggaggcaagt atgagatccg
     3001 aatcacagca gtcgacccag aaggcgcatc cggagaggcc ataagcgagg agttcacggt
     3061 aattgcactc actcgcactg tagtggctgc tccgaacccg acttctgatg tagtaacgtt
     3121 ctactacgac atcgcagcaa acggaacgct ctacgtatac gacatcgcag gacgcctcgt
     3181 gcactctgca gagctttcag ccacggcgaa tgcccatgaa tggaacctcc attcatcagg
     3241 caagccggtg gcaaacggcg tatacctcta cgtcgtgata gccggtgaag agagatctga
     3301 ggtcgggcgt cttgttgtga gcagatagat gcataggtct gggcggtcgc acaagcgact
     3361 gcccggactt cacagatttg cacgacctta aggagggcgg aaaatgcgaa tcaagaaaac
     3421 agtgcttgcg ctcgtgctcg taattgctct gattcccgca tcagctgcat ttgccgagga
     3481 cgatccggtg ggaacggcgg cgatgatggg acttggaatg ggggcgcggg cgcttggaat
     3541 gggcggcgcc catattgcgg tggcggatga cgcctccgtc atatactaca acccggcagg
     3601 cttgggtgta gtggatggta taagcgtcac atccctgtac acaaaccagt atggcgcagc
     3661 agggtatctc gcgttaggag gcgtggcacg acacatcggc gcgggaatcc tgaggctcga
     3721 cgcttccggg atagaagaaa cagatgaatt cgcaaacgag attggcacat ttggtgtggc
     3781 tagtaccgct gtgatggcag gctacggcat gacggtaatg ccgaacctga gcgtcggcgc
     3841 ggcggtcaag tactaccaag agacactgcc cgaaaactca gggaaaggct tcacggctga
     3901 catgggagtt ttgtattcca tggcggatga caagatccgc gtgggcgcgg tcggaaggaa
     3961 cctcttcggc aaggtggcct actccagtgg tgcggaggat gccttcgatc gcgcgttcgg
     4021 acttggagtt gccttcgagc ccatggagaa cttgctcgtt gcggcggatg ccgtgatgga
     4081 caacggattt tccggaaagg tgggggcaga gtacagattc cgccagatgg cgctccgcgc
     4141 aggcggctct tttggcggga atggaacatg cctcactgca gg
//
LOCUS       DAOSAI010000217         4171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13442_length_4171_cov_4.473032, whole genome shotgun sequence.
ACCESSION   DAOSAI010000217 DAOSAI010000000
VERSION     DAOSAI010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13442_length_4171_cov_4.473032"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1032)
                     /locus_tag="PLM83_07575"
     CDS             complement(64..1032)
                     /locus_tag="PLM83_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK71299.1"
                     /translation="MKYIFGPVPSRRLCRSLGVDLIPYKHCSFDCIYCQLGATVHKRA
                     ERQQFVETGEVIAELESALPCVEADYVTLSGSGEPTLCLGIGELIEGIKELTEIPVAV
                     LTNSSLMSCAEVRKELLGADLVIPSVDAATQDVFERINRPCSGLRIEDILCGLEEFSY
                     EYSGKLWIEVMMVRDINDSDDEIDKIASALTRIRCEKIQLNTVERPPAESFARALTQG
                     ALEKVRRRFDDRAELIVASGIYQDADGSFHSPAGAGLGAPGIEADCGRVLELLKRRPC
                     TVRDIAEGLDLNINEAAKFVGILKGEGLICAIRREGRAYFFANDGN"
     gene            complement(1162..1650)
                     /locus_tag="PLM83_07580"
     CDS             complement(1162..1650)
                     /locus_tag="PLM83_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOK71300.1"
                     /translation="MPRPPKCRRVEFMPEVTVFKPAGVRLAEMEEEILLLEELEALRL
                     KDVEGLEQEECADRMQVSRPTFQRVLSSAREKVARALVEGKAIRIEGGTYRLAARKLR
                     CEDCSYEFEAPVAMYRQGAGAACPRCGARHGRRNDGDQTAGGMMHHGRCGRGWRNTEG
                     DE"
     gene            2202..2591
                     /locus_tag="PLM83_07585"
     CDS             2202..2591
                     /locus_tag="PLM83_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOK71301.1"
                     /translation="MKNSTPISPSLEDYLEAVLEVADESGAARTTDIADKLGVSKASV
                     NQAMGLLVDRGLIEREKYGPVFLTDSGDEQARAVSRRHHTIKAFLVSVLGVAEHIAEE
                     DACQIEHVVSKDTMNGLVEFMERRDAE"
     gene            2588..2980
                     /locus_tag="PLM83_07590"
     CDS             2588..2980
                     /locus_tag="PLM83_07590"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOK71302.1"
                     /translation="MSDANGAISLSQLNPGDRGTILSIDCEGPLRRRLMDMGLTPGTE
                     VCIEGTAPFGDPLIVCARGCRLGLRRGEAAGISVIPCADRPCSEACKLENHGPFRRRH
                     RHGQVAASGDKCLRESGRHQHRKADCTE"
     gene            3108..>4171
                     /locus_tag="PLM83_07595"
     CDS             3108..>4171
                     /locus_tag="PLM83_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOK71303.1"
                     /translation="MNHSSAHSRPVIAIAGNPNSGKTALFNAMTGSRQHVGNWPGVTV
                     ERKEGTLTRGETSALVVDLPGTYAFSSRALDEVIARNFIMHEHPDVAINVVDATNLER
                     NLYLTLQMLEMGVPTIVALNMIDEAEELGLDIDIDELSNHLGVGVVPTAAVHGRGVEE
                     LVDLALKTANAVAGAEGLEDVEKPADAGTPSYWIDYGPILEKEIALLEQALAGVPDLC
                     RRRSARWLAIKLLEKDADILQEIRNSANEQANTAYGKLIAELEVAAEEACARVKKAAG
                     REAVDLIAEKRYELAAIISASVIVSRPDDAEITPTQRIDKFVLHPWLAYPIFFVVVWL
                     MFQITFTLSEPIADALSGLFA"
BASE COUNT         1008 a         1191 c         1076 g          896 t
ORIGIN      
        1 cagtataccc atggttgtga gcatatgtca ataatctgag cgcagaaatg tgcccgcaat
       61 ggttcagttt ccatcgttgg cgaagaaata ggcccgtcct tctcggcgga tggcgcagat
      121 aagtccttct cccttgagaa tccccacaaa cttagcggcc tcattgatgt tgagatcgag
      181 cccttcggct atgtccctga cggtgcacgg ccgtcttttg agtagctcaa gcacccgccc
      241 acagtctgct tcgatcccgg gcgcccccag cccggccccg gctgggctat gaaaagaccc
      301 atcagcgtcc tgataaatcc cggacgccac gatcaactcc gcccgatcgt caaaccgtct
      361 tctcactttt tccagcgccc cctgtgtcag tgcgcgggca aaggattcgg cgggaggcct
      421 ttccaccgta ttgagctgga tcttttcaca ccttatgcgt gttagggcag aggctatctt
      481 gtcgatttca tcatcactgt cgtttatgtc cctgaccatc atcacttcta tccaaagctt
      541 ccccgagtac tcatacgaga actcctccaa gccgcagagg atgtcctcga ttctcaatcc
      601 ggagcatggg cggtttatcc tttcaaaaac gtcttgcgtt gcggcatcaa cagaaggaat
      661 cacaaggtcc gcgccaagga gctcttttcg gacctcagca catgacatga gcgagctatt
      721 ggtcagaacc gccacaggaa tctctgtcaa ttcctttatc ccctcgatca gctcccctat
      781 ccccaagcat agggtgggtt cgcccgaccc cgacaaagtg acgtagtcgg cttccacaca
      841 gggcaaagcc gactcgagct cggcgatgac ctcgcctgtc tcgacgaatt gctggcgctc
      901 cgccctcttg tggacggtcg cgccgagctg gcaatagatg cagtcgaagg aacaatgctt
      961 gtacggaatc aagtcgaccc ccaacgaacg gcacagccgt cttgagggaa ctggaccaaa
     1021 gatgtatttc aaactcaacg ccccccgtat actctggttg cctcatttgg ccccggcgaa
     1081 acaagcaaag gtttttggca aggtgtaaag gtcagttgcc tggcatgtgg ccgctttgga
     1141 tgcaccttga agctttaacc tctattcgtc gccttcggta ttcctccacc ccctgccaca
     1201 gcgcccgtgg tgcatcatgc cgcctgctgt ctggtccccg tcgttacgtc gcccatgacg
     1261 cgcaccgcac ctagggcaag ccgctccggc gccttggcga tacatggcaa cgggtgcctc
     1321 gaactcataa ctgcaatcct cgcaccgcag cttccgcgca gcaagacggt aggttccgcc
     1381 ttcgatccgg atagccttac cttcgaccag cgcccgggcc accttctccc tggctgaaga
     1441 gagcaccctc tggaatgtag gccgggacac ttgcatccta tcggcgcact cctcttgctc
     1501 tagcccttca acatccttga ggcgcaaagc ctccagttct tccaggagga ggatctcctc
     1561 ttccatctcc gccaatcgaa cgccagcagg cttgaagaca gtcacttcgg gcatgaactc
     1621 gactcttctg cattttggag gcctcggcat aatatcagct cctaatcaga gatctttcaa
     1681 gttttcttca atcggcgcaa cagcaatcta agccgcttac aatcgaatat tgcgggatcc
     1741 tgaaattcac gttccttggg gttagggccc gcgtgcgata tagtcgccca aggcgatgtt
     1801 ttaaggctac aaaggaaagc cccaggctca actgcaactt gcaaagagac aaccaggtgc
     1861 cctctcatcc catctacacc catcctgagc acgcaattga ctatggctaa cctgcactga
     1921 acgcacatga gtccttcaca actatcttcc cgtcccagga cgtatcacac ctggatagag
     1981 ttgataaagg ccagtcgaga cgggtagatt atattgttgc atattgatca tatgtccata
     2041 attattttcc cgctacggca cgtacatgtc aatacgcgtc agaaagctct tgacatctcc
     2101 tgaaaaatgg catagattct tatcagggca agttagtcat gccttactta gctttcggct
     2161 gatccagatc ggccgcctca gtatgaaggt gggagaaaga agtgaagaac tcaacgccaa
     2221 tatcgccatc gctcgaggat tacctcgaag ccgtgctgga agtggcggac gaaagcggtg
     2281 ctgcccgtac caccgatata gctgataagc tcggcgtatc gaaagccagc gtcaatcagg
     2341 caatggggct tttggtcgat cgcggcctaa ttgagcgcga aaagtacggt ccagtctttc
     2401 tcacagactc cggggacgaa caggctcgag ccgtgtccag gcgccaccac accatcaaag
     2461 cctttctcgt atcggtgcta ggcgtggccg aacacatagc agaagaagat gcgtgccaga
     2521 tagagcatgt agtcagcaaa gacacaatga acggactagt tgaattcatg gagaggagag
     2581 acgcggaatg agcgatgcta acggagcaat atccctcagc caactcaatc cgggcgatcg
     2641 gggaacaatc ctgagcatag attgtgaggg accactcagg agacggttga tggatatggg
     2701 cttgacccct ggaacagaag tctgcataga agggacagcg cccttcggag atcccctgat
     2761 agtgtgtgcc cgcggctgcc gcctcggtct gcgaagaggc gaagcggcag gcatatccgt
     2821 cataccgtgt gctgacaggc cctgctcgga agcatgcaag ctggaaaatc acgggccgtt
     2881 ccgtcgacgc cacagacatg gacaggtggc ggcctcaggc gataaatgcc tgcgggaaag
     2941 cgggcgccat caacacagga aagcagattg cactgaatag agaaatactc gcacgggcgt
     3001 ccattgggcg cactcttttt ggaccaaaag ttaggcgttg gtaactatta tgcgtctttt
     3061 aggctttata cattatttac acgaatcatc tacggaggca caatgagatg aaccattcaa
     3121 gtgcacactc taggcctgtc atagcaattg cgggcaaccc caactcaggg aagacagccc
     3181 ttttcaatgc aatgacaggg tcaagacaac acgtgggcaa ttggcccgga gtcacagtag
     3241 agcgaaaaga gggcacattg acaagaggcg aaacctcagc attagtcgtg gacctgccgg
     3301 gtacttacgc tttcagctcg cgagcgctgg atgaggtaat tgcccgtaac ttcatcatgc
     3361 acgagcaccc agacgtggca ataaacgtcg ttgacgctac gaatctcgaa agaaacctct
     3421 acctaaccct tcagatgctg gagatgggcg tgcccaccat tgtggcactg aacatgatag
     3481 acgaggctga agagctcggc ctcgatatag acatagatga gctgtcgaac cacctcggag
     3541 tcggggtcgt gcccacagct gccgttcatg gaagaggagt cgaggaacta gtcgatctcg
     3601 ccctcaagac tgccaatgcg gtcgccgggg ctgaaggcct cgaagatgta gaaaaacccg
     3661 ccgatgccgg aacgccttca tactggatag actacggccc cattttggag aaggaaattg
     3721 ccctgctcga gcaggcactt gccggcgtcc cggatctctg ccgtagacga tctgcgcggt
     3781 ggctggccat aaagctgctt gaaaaagacg ccgacatttt gcaggaaatc aggaattcag
     3841 cgaatgaaca ggccaacacg gcctatggaa agctaattgc cgaactcgaa gtcgcagcag
     3901 aggaggcctg cgcgcgcgta aagaaggcag caggtcgtga agctgtggac ttgattgccg
     3961 aaaagcgcta cgaacttgcg gcaatcatct ccgcgtccgt catagtctca cgcccagatg
     4021 acgccgagat cactcctacg cagcgtatcg acaagttcgt gctccatccg tggctcgcat
     4081 atcccatatt cttcgtcgta gtatggctaa tgtttcagat aacatttacc ctcagcgagc
     4141 caattgcaga cgcactcagc ggcttatttg c
//
LOCUS       DAOSAI010000218         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13509_length_4156_cov_3.589368, whole genome shotgun sequence.
ACCESSION   DAOSAI010000218 DAOSAI010000000
VERSION     DAOSAI010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13509_length_4156_cov_3.589368"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="PLM83_07600"
     CDS             <1..1243
                     /locus_tag="PLM83_07600"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOK71304.1"
                     /translation="MGETTIGKRSELSTDIGLLYIDTLQILGLQRAKTAREAVQIMGE
                     LAETYGYIDGGECLTVGDPNEIWHFEVMPPGNFQKGAIWAAVRIPEGHVGVSANRSRI
                     CEVNPDDPENYMCSSNVFDVAIENGWWDPESGEEFLFWKAYCPSNWFGCKLREWRVLS
                     MVAPSLNLDPNADRYRFTVKAERPITVQDMMNIYRDHYQGTEFDMADAPGWYLKDAEG
                     NPVVDKQGNPVKTPYAQPNPGTARRQHIGIPVNMRNIAVPYCSYFTIVQARDWLPDPV
                     GGLCWFGLSNPLSSVYVPIYCGVDSLPKNYAVNDRKVFTFDSMKESAWWVFEYMDNLV
                     SRRYVDILADLNKVREPFEAEQFELQPVIEKAAVELYEKDPALAKTFLTNYTHSRCAK
                     AVDTYYRTAEELTLKWNDNTF"
     gene            1374..2006
                     /locus_tag="PLM83_07605"
     CDS             1374..2006
                     /locus_tag="PLM83_07605"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOK71305.1"
                     /translation="MKHSSVRRTILIAIAAVMSMILSTGVCFGSVSKPAGIEIPAKLP
                     RLDQPVLITSIGQAPGASWAKVLMKQLKIDVTHDDMVTAEDLVKASKNPKTAYKTLIM
                     SMGTSLKGMGGAGVDIDKEVARCNALADQAKKLGMTIVGIQIEGSARRTDESDEKSNR
                     AVTSKSDILIVRKEVDKDGFFTNAAKQKGIPIIRTVESMDFKYAFSVLFR"
     gene            2060..3346
                     /locus_tag="PLM83_07610"
     CDS             2060..3346
                     /locus_tag="PLM83_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HOK71306.1"
                     /translation="MYAHAAMVLAVIAVAFVIAILLRLGTEISMLIAAIAGAIAHGAG
                     IPARHIVEGTFTYFDVVFIFITATFFMNLLKEAGGVTYIVRGIISKFHKNRVALLLLL
                     TLIMLVPGALTGSGTVTVLVVGSFVGTVLSYMGIPKDRVAAAVFMGSAMSAAAPPINL
                     WAMMAAAGANMPYVGFTLPLGIISVAGALFSIFYLARGSKPIDAEQALKEIPEAPEGM
                     AWYKVAIPFVVFFGIIVLGRQFPFSMPVIGLPLAFMLSALAVVLLSPVKLRVFEILSN
                     TVEDLLPLISTVTIVGILVQIMALSGARGLISLSVVTMPLAAIFAALFVVLPVSEGIF
                     QYASAPLLGVPLVFLLNMKGYDPVIALAGMASMWPLGDCLPPTAPVGRGAVMAVGYDG
                     DYKAFLKQCLVPALFVLVIGTLFVVFSPKLGFLARG"
     gene            3366..3521
                     /locus_tag="PLM83_07615"
     CDS             3366..3521
                     /locus_tag="PLM83_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71307.1"
                     /translation="MQSVISVLYYVITAMITAVLMVNMFRAKDIKKVILYAAVLIPFA
                     LRVARIK"
     gene            3550..>4156
                     /locus_tag="PLM83_07620"
     CDS             3550..>4156
                     /locus_tag="PLM83_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HOK71308.1"
                     /translation="MSKKKDQNGVNRWAMLIVIVLWLAVVIPGGKEIYGHRHFDEPVV
                     LGPGVTEVKKLSDYSPAVKGTINDCNIYVLDSGEPGGTVFVLGGTHPEEPAGVLSAVT
                     IVENVIPTNGKILVAVRGNYSASTVTRPGDAYPLYYSIKTDFGERTFRMGDRWSNPLD
                     SWPDPEVYINYPSKQLLAYMDVRNLNRTWPGKLYGGGWICAT"
BASE COUNT         1015 a         1082 c         1141 g          918 t
ORIGIN      
        1 catgggcgaa acgaccatcg gcaagcggtc agagctcagc accgacatag ggcttcttta
       61 tatagatacc ctccagatcc tcggactgca gagagcgaag acggcacgag aggcagtcca
      121 gatcatgggc gaactagccg agacttatgg ctacatcgat ggcggcgaat gcctcaccgt
      181 gggagacccg aacgaaatat ggcactttga agttatgcct ccaggcaact tccagaaggg
      241 cgccatttgg gccgccgtcc gcatccccga aggccatgtt ggcgtttcag ccaacagaag
      301 ccgaatctgc gaggtcaacc cggacgatcc agagaactat atgtgctcat ccaacgtatt
      361 tgatgtagcc atcgaaaatg gctggtggga tccggaaagc ggcgaagagt tcttgttctg
      421 gaaggcgtac tgccccagca actggtttgg atgcaagctc cgcgagtgga gagtcctctc
      481 aatggtagct ccctccctta acctagatcc caatgccgac aggtatcgct tcaccgtcaa
      541 agccgagcgg ccaatcactg ttcaggacat gatgaacatc tatcgcgatc actatcaggg
      601 cacagaattc gatatggccg acgccccagg atggtatctg aaggatgccg aaggtaaccc
      661 tgttgtcgac aaacagggta accctgtgaa gaccccatac gcgcagccca acccaggcac
      721 cgcacgaaga cagcatatag gaatcccggt gaacatgcga aacatcgcag ttccctactg
      781 ctcctacttc acgatagtcc aagcacgcga ctggctccct gatccagtgg gcggcctttg
      841 ctggtttggt ctgagcaacc cgctgtcatc cgtgtatgta ccgatctact gcggagtcga
      901 cagcctgcca aagaactacg ctgttaacga cagaaaggtc tttaccttcg acagcatgaa
      961 ggaatcggca tggtgggtgt ttgagtacat ggacaacctc gtcagccgaa gatacgtcga
     1021 catattggcc gacctcaaca aggtgagaga accgttcgag gccgagcaat tcgagcttca
     1081 gccggtgatc gagaaggctg ccgtggagct ctatgagaaa gaccccgctc tggccaagac
     1141 attcctgaca aactacacac attcgagatg tgccaaggcg gttgacacct actatcggac
     1201 ggccgaagaa ctgacgttga agtggaacga caacaccttc tagtcatctg gcctttcatc
     1261 tgtggacgat gatccgcaga attgtcctcg gtttcgccag atggcgaaac cgagggctaa
     1321 atcgtagtag gtcaacacaa gtgcttcagg aaaagaaagg agatttacgc aacatgaaac
     1381 actcttctgt tcgacgaaca atcctgattg ccattgcagc agtcatgagc atgattctgt
     1441 caacaggcgt atgcttcggc tcagtcagca agcccgcagg gatcgagatt cctgcaaagt
     1501 tacccaggct cgaccagccg gttctcataa cctcaattgg acaggctccg ggcgcatcat
     1561 gggctaaagt gctgatgaag cagctgaaga tcgacgtcac ccatgacgat atggttaccg
     1621 ccgaagactt ggtaaaggcg agcaagaacc cgaagaccgc ctacaagacc ctgataatgt
     1681 ccatgggaac cagcttgaag ggaatgggcg gcgcaggggt agatatcgac aaggaagtcg
     1741 caagatgcaa cgccctggct gatcaggcta agaagctcgg aatgaccata gtaggaattc
     1801 agatagaggg aagcgcgcgg cggacggacg agtccgacga gaaatcgaac agagcagtca
     1861 catcgaagtc cgatattctc atcgtgagga aggaagtaga caaggacggc ttcttcacga
     1921 acgcagcaaa gcagaagggg attccgatca tcaggaccgt tgaatccatg gatttcaagt
     1981 acgcgttcag cgttctgttt agataataga caagaaagct taacgcctta atttgtacta
     2041 ggacgaggtg gaataacctg tgtatgctca cgcagctatg gtcctggcgg tgatagccgt
     2101 cgccttcgtg atcgcgatcc tactccgctt gggcactgaa atatctatgc tcattgcggc
     2161 aatagcaggc gccatcgcgc atggggcagg gatccctgcc cgccacatcg tggaggggac
     2221 cttcacatat ttcgatgtgg ttttcatatt catcacagct acgttcttca tgaacctgct
     2281 gaaggaagca ggaggagtaa cctacattgt gagaggcatt atatcgaagt ttcacaagaa
     2341 ccgtgtggcg cttcttctgc ttcttacact aataatgctc gttcccggcg ccctgaccgg
     2401 atccggcaca gtgactgtgc tcgtcgtcgg atcattcgtg ggcactgtcc taagctatat
     2461 gggcatccct aaagatcggg tcgcagccgc cgtgttcatg ggatcggcaa tgagcgcagc
     2521 tgcgcctccg atcaaccttt gggcgatgat ggcggcggcc ggcgctaaca tgccctatgt
     2581 tggattcact ctgcctctcg gcataatatc tgtcgcaggc gcactgttct ccatattcta
     2641 tttggcgcgc gggagcaagc ccatagatgc cgagcaagct ttgaaagaaa ttcctgaggc
     2701 gcctgaaggc atggcttggt acaaggtggc gataccattt gtcgtcttct tcggcatcat
     2761 cgtactgggc agacagtttc ccttttcgat gccggttata ggccttccgc ttgccttcat
     2821 gctctcagct ctggcagtag tgctcctatc tccggtcaag ttgcgagtat tcgagatcct
     2881 ttcgaataca gtcgaggatc ttctgccact gataagcaca gtaacaatag tggggatact
     2941 cgtgcagatc atggctctgt cgggcgcaag aggcttgata tctctttcgg tggtcaccat
     3001 gccgctggca gccatattcg cggcgctctt cgtggtgctt ccggtatccg agggaatatt
     3061 ccagtatgct tcggcccctc tgttgggagt tccgttggtg ttccttctca acatgaaggg
     3121 ttatgatccc gtaatagctc tggcgggcat ggcgtcaatg tggccattgg gagactgcct
     3181 gccgccgact gcccctgttg gaaggggagc tgtcatggct gtgggatacg atggagacta
     3241 caaggccttc ctcaagcagt gcctcgtgcc ggcgctgttc gtgttggtca taggaacgct
     3301 gttcgtagtc ttcagcccaa agctgggatt tttggcccgt ggatgaacga acggaggaat
     3361 gtcacatgca gagcgttata agcgttttgt actatgtaat tacggcaatg ataaccgccg
     3421 tgctcatggt taacatgttc cgcgccaaag acatcaaaaa ggtgatcttg tatgccgcgg
     3481 tgctaatccc attcgcgctt cgtgtagcgc gcatcaagta gaggctgtgg aaggaggcgg
     3541 catacacgca tgtctaagaa gaaagatcaa aatggcgtaa acagatgggc aatgctcatt
     3601 gtcatcgtcc tatggcttgc cgtcgtgatt cccggcggaa aggagatata cgggcatcgc
     3661 cacttcgatg aacccgtagt gctcggacct ggagtcaccg aagtcaagaa gctgtcagac
     3721 tactctccag cagtcaaagg taccatcaat gactgcaaca tatacgtgct tgacagtggc
     3781 gaacccgggg gaaccgtgtt cgttttaggt ggaacacacc ctgaggaacc cgcgggcgtg
     3841 ctttccgctg tgacaatagt ggagaacgtg atccccacta atgggaaaat cctcgttgcg
     3901 gtgcgcggaa attacagcgc atccacggta accaggccgg gcgatgcata tccgctgtat
     3961 tacagcatca aaacggattt tggcgaaaga acgttcagga tgggagacag gtggagcaat
     4021 cccctcgact cctggccaga tcctgaggtt tacatcaact atcctagcaa gcaattgctt
     4081 gcatacatgg atgttcgcaa cctcaaccgc acctggcccg gcaagctgta cggcggcggc
     4141 tggatctgcg ccacct
//
LOCUS       DAOSAI010000219         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13564_length_4142_cov_3.036212, whole genome shotgun sequence.
ACCESSION   DAOSAI010000219 DAOSAI010000000
VERSION     DAOSAI010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13564_length_4142_cov_3.036212"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLM83_07625"
     CDS             complement(<1..325)
                     /locus_tag="PLM83_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HOK71309.1"
                     /translation="MTDSLKIDGLLMEAVNLGASDLHLTTGLPPMVRVSGALKALEGH
                     LPLKPADTREMMYSIMKPDIRERYEAAGEVDFSHGMPGVARFRVNANTQRGSVGAAFR
                     VIPTRI"
     gene            complement(380..2119)
                     /locus_tag="PLM83_07630"
     CDS             complement(380..2119)
                     /locus_tag="PLM83_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOK71310.1"
                     /translation="MTRPGHKRIGDYLLEAGALTEDELEEALEIQRRTGERLGRVLVK
                     LGHVSESSIATVIERQLGIPRVSLAEYVVADDVIKIVPENMARRYKVLAVERDGNKLT
                     LAMADPLNVLAIDDIKLATGFEVSPVVAPEDEIVRALDQYYGSSEDLDEVVRYLEKYS
                     EDAIRFASYEEEIGVDRLRELVDEAPVVRLVNMIIAQAVGERASDIHVEPHATGVRVR
                     YRVDGILREVMDLPRGSHAAIASRFKIMSNMDIAERRVPQDGRVQLKVEGEEIDLRVS
                     TLPTIYGEKVVCRVLFRRGAMVKLEQLGFLPDNLELWHRALSCPYGIILVTGPTGSGK
                     TQTLYASLNQLNSVEKNIITIEDPVEFRLDGINQVQTNPKAGLTFASGLRSMLRQDPD
                     IIMVGEIRDRDTAEIAVNAALTGHLVLSTLHTNDAPGATVRLVDMGAEPFLVASSLIC
                     VLSQRLVRKVCTHCREDYVPDDEEWGRWVRMTRGLSDESGLGLPRRLVRGKGCRQCGN
                     TGYRGRTAIHEIMMVTEPIRELVSGNASSDVVAEVAREQGMRRLIEDGADKVRMGITT
                     LDEVMRVAAGSEV"
     gene            complement(2248..3444)
                     /gene="coaBC"
                     /locus_tag="PLM83_07635"
     CDS             complement(2248..3444)
                     /gene="coaBC"
                     /locus_tag="PLM83_07635"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOK71311.1"
                     /translation="MTGTCVLGVTGGIAAAKASLLASMLVKQGLDVHVIMTEAVCEFV
                     APLTFRTLTANPVSVDMFAEPGEWNVKHVSLAEAADVFVVAPATANFIGKVASGIADD
                     LLTTTIMATRAPRVIAPAMNSGMYANPIVQRNIETLSALGYRFIGPESGRLACGDVGA
                     GRLAEPERILDFVLHVCAGTGRPSLAGLRVVVTAGPTREPLDPVRFITNRSSGKMGYA
                     IAGAAAVRGAKVTLVSGPVFLPAPEGCEMVRVETACQMRDATIDAAQEADVVVAAAAV
                     ADYTPASFSESKIKKSSGFGSLELSRTPDILAELGSAKGERVLVGFAAETDDLLSNAE
                     RKLSSKNLDMIVANMVGGEDSPFGADHNTVTILCPGAPPETFPAMSKLVLAHEILDRV
                     SAICHH"
     gene            complement(3576..3761)
                     /gene="rpoZ"
                     /locus_tag="PLM83_07640"
                     /pseudo
     CDS             complement(3576..3761)
                     /gene="rpoZ"
                     /locus_tag="PLM83_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            complement(3875..>4142)
                     /locus_tag="PLM83_07645"
     CDS             complement(3875..>4142)
                     /locus_tag="PLM83_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOK71312.1"
                     /translation="FLMPPSLAELEARLAKRGADSPEATRRRLRKAIEEIPAVLKYDY
                     VVLNYDLEQAAEQIRSIIVAERARVGRCDCEHFIRGFEMESQIF"
BASE COUNT          880 a         1360 c          993 g          909 t
ORIGIN      
        1 tgatccttgt tggaatgacc ctgaacgctg cccccacgga gccacgctgc gtattagcgt
       61 tcaccctgaa cctggcaacc cccggcatac cgtgggagaa gtcgacctct cccgccgcct
      121 catacctctc ccgaatgtcc ggcttcatga tgctgtacat catttcgcga gtatcagccg
      181 gtttgagtgg aagatgccct tccagggctt tcaaagctcc gctcaccctc accattggag
      241 gaagccctgt agtgaggtga aggtctgaag ccccgagatt taccgcctcc ataagcaatc
      301 cgtctatctt caagctatca gtcacctatg atatcactct cccgaaaagc aaaagcccca
      361 tatgtacaga tggaatcgcc tagacttccg acccggccgc caccctcata acctcgtcca
      421 aagtagtaat ccccattcgg accttgtccg caccatcctc gatgagcctt ctcatcccct
      481 gctccctggc gacttccgcc acaacgtcac tggaagcgtt gcccgacact agctcccgta
      541 tgggctccgt caccatcatg atctcatgta ttgccgtccg tccccggtac cccgtgttgc
      601 cgcactggcg acaccctttc ccgcgcacaa ggcggcgagg gagccccaag ccggattcat
      661 ctgaaagccc acgcgtcatc ctgacccacc tgccccactc ctcgtcgtcg gggacataat
      721 cttccctaca gtgcgtgcaa accttgcgga caagcctttg cgataatacg catatcaaag
      781 atgacgcgac caaaaacggc tcagccccca tatccactaa acgcacagtt gcgcccggcg
      841 cgtcatttgt gtggagcgtc gagaggacca ggtggcccgt gagagccgca ttgactgcga
      901 tctcggcggt atcgcgatcc cggatctctc ccaccattat gatgtcgggg tcctgtctca
      961 gcatcgatct gagccctgaa gcaaaagtca ggcccgcctt gggattcgtc tgcacctgat
     1021 ttatcccgtc aagcctgaac tccacaggat cctctatagt gattatgttc ttctcaaccg
     1081 agttcaactg gttcagcgat gcatacaacg tctgcgtctt cccgcttcct gtggggcccg
     1141 tgaccagaat aatgccatag gggcaagaaa gcgcccgatg ccaaagctcc aggttgtcgg
     1201 ggagaaaccc aagttgctcc agtttgacca tggccccgcg cctgaacagc acgcggcaga
     1261 cgaccttctc tccatagatg gtgggcagcg tcgacaccct gaggtctatc tcttcgccct
     1321 ccaccttcag ctgaactctg ccgtcctgag gcacgcgcct ttcagctata tccatattgc
     1381 tcattatctt gaaccgcgag gcgatcgccg catggcttcc cctgggaaga tccatgacct
     1441 cgcggagaat tccgtctact ctgtatctga ccctgacccc tgtggcatgc ggctccacat
     1501 gaatatctga cgcccgttct cccacagcct gggcgattat catgttgacc agcctaacca
     1561 cgggagcttc gtcaacgagc tcgcgcagac ggtcaacgcc tatctcctct tcgtagctgg
     1621 caaaccggat ggcatcttca gagtatttct ccaggtagcg tactacttca tccaggtcct
     1681 cagaagaacc gtagtactga tctagcgcgc gaactatttc gtcctcaggt gcaactacag
     1741 gactgacttc gaacccggta gccagcttta tgtcgtcaat cgccagaaca ttgagaggat
     1801 cagccatggc aagggtcagt ttgttgccgt ccctttcgac agcaagaacc ttgtaccttc
     1861 ttgccatgtt ttcgggaaca atcttgatca cgtcatcagc aaccacatac tccgcaaggc
     1921 tcactctggg aatgcctagc tgccgctcga tcacagttgc aatcgaactc tcgctcacat
     1981 gccccaactt cacgaggacc ctgcccaagc gctcccctgt gcgccgctga atctcgagcg
     2041 cctcttcgag ttcgtcttcg gtgagcgccc ccgcctcgag caaataatcg ccaattcgtt
     2101 tgtggccggg tcgcgtcaaa gggcatcagg cctccccatc agttgactac gagtaacgtt
     2161 ctacgtacat tttcgtattc cttctacatt actccataaa tgcagaagcc tcccttatat
     2221 caggaccaat tatcctcaat ttgcctctta gtggtggcag atggcagaaa ccctatccag
     2281 aatctcgtgc gccagcacga gcttggacat tgcaggaaat gtctcgggcg gtgcgcccgg
     2341 gcagaggatt gtgacagtgt tatgatcggc cccgaaagga gagtcctcac cgccgaccat
     2401 gttggcgact atcatgtcga gattcttgga ggaaagtttt cgctcagcat tggagagcaa
     2461 gtcatctgtt tcagcagcaa aacccacgag tacgcgctct ccctttgcac tccccagctc
     2521 ggccaggata tcaggcgtgc gcgagagctc caaagaaccg aagcccgatg actttttgat
     2581 cttgctctcg ctgaaagaag ccggcgtgta gtcggcaacg gccgcagccg caacgaccac
     2641 gtccgcctct tgcgcagcat cgatcgtcgc atcccgcatc tggcaggcgg tttcgactct
     2701 gaccatctca cacccctcgg gcgccggaag gaatacagga cccgaaacca aagtcacctt
     2761 cgctccccgc acagccgccg cgcctgcgat cgcatacccc atcttcccag atgacctgtt
     2821 ggtaatgaac cggacgggat ctaggggctc cctagttggt ccggcggtca caaccacccg
     2881 cagtcccgca agtgacggcc ggcccgtccc tgcacacaca tggagaacga aatcgagaat
     2941 tctctccggc tccgcaagcc tgcccgcgcc cacatcgcca caggccagcc tgcccgactc
     3001 gggcccgatg aatctatagc ccagcgccga aagggtctct atattccgct ggacgatggg
     3061 gtttgcgtac atgccagaat tcatggccgg cgctatcacc cggggggcgc gcgtggccat
     3121 gatcgtcgta gtaagcaagt catcagctat cccagatgcg accttcccga tgaagttggc
     3181 cgttgccgga gccaccacga aaacatcagc ggcttccgca agggatacgt gcttcacgtt
     3241 ccactcgcca ggctcggcaa acatgtctac gcttacagga ttggctgtga gcgtcctgaa
     3301 ggtgagaggc gcaacaaact cgcacactgc ctccgtcatg atcacatgta catcaagccc
     3361 ttgcttgacc agcatgcttg caagaagcga agctttggcc gccgctatcc ctccggtcac
     3421 ccccaacaca cacgtgccag tcacgggcga tcacactcct aaaaaagcgg ggcacgctcc
     3481 aaatagtcat cggtaggctt cagctgctcc ctgccgctgg gctgttccac ccagccgtca
     3541 tatttcttgg gtatgccggt ttcgccaggg ctaggcctat aatacgtcac ctttccctcg
     3601 gctatctccc gcagcgcgcg ggttacaggc ttttcaggac catactctat tacgtccttc
     3661 tgcgccgaca ttatcttcct tgcccgtctg gccgccatta ccaccagcgt atacctgctg
     3721 tcgctatgat ccatcagttc ttccaatgag ggtcgaatca ctcgagtagc ctccttatgc
     3781 atgcggcggc ggcgccgccg cacgtacctt gtaaaagccc ccttcatccg ccatccgggt
     3841 ctgtggcgtc agggaggctc aacaccatat tccctcagaa tatctgcgat tccatctcaa
     3901 aaccgcggat aaaatgctca cagtcgcatc tgcccacccg ggcccgctcc gcgactataa
     3961 tagaccggat ctgctctgcg gcctgctcca aatcatagtt gagaaccaca taatcatatt
     4021 tcagcacggc aggtatctcc tctatagcct tcctaaggcg tcttcttgtc gcctcaggcg
     4081 aatcagcgcc ccgctttgcc agccttgcct ctagttctgc aagggacggc ggcatcagaa
     4141 ag
//
LOCUS       DAOSAI010000220         4141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13569_length_4141_cov_3.966471, whole genome shotgun sequence.
ACCESSION   DAOSAI010000220 DAOSAI010000000
VERSION     DAOSAI010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13569_length_4141_cov_3.966471"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLM83_07650"
     CDS             <1..825
                     /locus_tag="PLM83_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOK71313.1"
                     /translation="NRDRIAVVLIAGPSSSGKTTFAQRLWVQLRVNGLNPVPLSLDDY
                     FVDRANTPVDEEGEYDFETIEAIDIPLLNEHITRLIQGEEVEVPVFDFIEGKRSSKGR
                     PIKLGQDQMLIIEGIHGLNDELTRSIPMGRKFKIYASALTHLNIDDHNRIPTTDVRIL
                     RRLVRDNLFRGHSAKDTIRMWPKVRAGEERNIFPFQETADVMFNTALAYELAVLKPYA
                     DPLLAAIGQEDPEYIEAKRLRKFLAYILPVERADIPPNSIMREFLGGSCFGRMRDA"
     gene            930..2024
                     /locus_tag="PLM83_07655"
     CDS             930..2024
                     /locus_tag="PLM83_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOK71314.1"
                     /translation="MRIGIVGMPGAGKTTLFRLLTGVAPSRKQDASIGMARVPDARID
                     LLSKMYQPKKTTYAQLEVVDLAGRASSGSTFDDFSSNKREIARGLTLLINHMRNVDAL
                     VNVVRTFDSLELGEPNPLADASALTDELILADMAIAEARIERLNSSKKRTEEEDKELA
                     LMERLDEEFGAGRSLRDIQLEPEEAAGLRGYGLLSVKPIVVVANMSEEQFREGKFPRD
                     SQLQEYCCERSIPLIRLAAEIEAEIAELDSDDKDLFMAAYGIEVSGIEQVSRAVYDSL
                     GLVSFFTVGEDEVRAWPIENGTTAKRAAGKIHSDIERGFIRAEVVACDDLMSAGSLAD
                     ARSAGSVRLEGKEYVMADGDIVNFRFSPAH"
     gene            2370..2603
                     /locus_tag="PLM83_07660"
     CDS             2370..2603
                     /locus_tag="PLM83_07660"
                     /inference="COORDINATES: protein motif:HMM:NF019490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HOK71315.1"
                     /translation="MDQNRLSDRDIAADLLSDLKELALGYHMATVEASNQSVRETFKN
                     SHDDWMNEQRGLWQTMYSKGWYQLPQTGPGKGM"
     gene            2776..3477
                     /locus_tag="PLM83_07665"
     CDS             2776..3477
                     /locus_tag="PLM83_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOK71316.1"
                     /translation="MGMKLIAFDMDGTLTQEPSSWEYVHRRFGLWTGRAEAHMMRFLS
                     GEITYEEFSILDAQEWKGMLHAEIERTLGEIHYARCAQEVVDAARSHGAFTALISSGI
                     LALARRVASDLGIDRVFANELVAREGVMTGDVVINVSIDEPHMTKGAVLRRLKMELGA
                     DTDETWAVGDNWGDIGMFREAGTAVLVAPTPEDREKVVVAVPNVVCVPSLCHVVSLIS
                     RIGAGDSCSPGATGG"
     gene            complement(3403..>4141)
                     /locus_tag="PLM83_07670"
     CDS             complement(3403..>4141)
                     /locus_tag="PLM83_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK71317.1"
                     /translation="FVGATGAGKTSITSLINRLYDIQKGQILIDGVDIRSYRVADLRR
                     NIAAVLQDVFLFAGDIKSNIRLNNVDISDEKIKEVARYVNADHFISKLPNGYDEPVTE
                     RGSTLSAGQRQLLAFARALASDPSILVLDEATANIDTETELLIQDALPKLIAGRTTIV
                     VAHRLSTIQHADKIIVIHKGRIREEGTHQELLAKRGLYYDLYRLQYKDELMPEPELDM
                     QDTSAAGCARTAGIASSDSADKTDDMA"
BASE COUNT          983 a          934 c         1253 g          971 t
ORIGIN      
        1 aaccgggaca ggatagcggt agtgctcatt gctggccctt catcctcggg aaagaccact
       61 ttcgctcaac gcctgtgggt ccagttgaga gtgaatgggc tgaaccctgt tccactctct
      121 ctcgacgact acttcgtcga tcgcgcgaat acccccgtcg acgaagaggg cgaatacgac
      181 ttcgagacca tagaggcaat cgatattccg ttgctcaatg agcacattac ccgcctgatt
      241 cagggcgaag aggtcgaagt tcctgtcttt gattttatcg aaggcaagcg gtcctcgaag
      301 ggtcgtccca tcaaacttgg acaggatcag atgctcatca ttgaggggat acacgggctc
      361 aacgatgagt tgactagatc tattccgatg ggccgaaaat tcaagatata tgcatcggcc
      421 cttactcatc tcaatattga tgatcacaac cggataccaa cgactgatgt gaggatcctg
      481 aggaggctgg tgagggacaa tctcttccgt gggcacagcg cgaaggacac cataaggatg
      541 tggcctaagg ttagggctgg agaggaaagg aacattttcc ctttccaaga gactgccgat
      601 gtgatgttca atacggcgtt agcctatgag cttgcggtgt tgaagccata tgccgatccg
      661 ctgttggccg cgattggaca ggaggatccc gagtatatag aggctaagcg tttgcgcaag
      721 ttccttgcgt acatccttcc ggtagagagg gctgacattc ctcctaattc catcatgcgg
      781 gagtttcttg gagggtcctg ttttggaaga atgcgtgacg cctgatgtag tgttttattc
      841 ttctgtagga tcgtggaaat ggataaagaa atgagcaacc cttcgttagg acgaggcttg
      901 cgagtagaca acgagaaggt gcaggttgta tgagaattgg catagtgggc atgccgggag
      961 cggggaagac gaccctattt cgtctcctga ccggagttgc cccgtcgcgc aaacaggatg
     1021 cgagcatcgg gatggctcga gttccggatg ctcgtatcga tcttttgagc aagatgtatc
     1081 agcctaagaa gactacttat gcacagctag aggtagtgga cttggcagga agggcatcat
     1141 ctggatcaac gttcgacgac tttagctcga acaaacgtga aattgcacgg gggttaacac
     1201 tgctcatcaa ccatatgagg aatgttgacg ctcttgtaaa cgtagtccgc acattcgact
     1261 ccttggagct gggagaaccc aatccccttg cagatgcgtc ggcgctgacc gacgagctaa
     1321 tcctggctga catggctatt gcagaagccc ggatagagcg gttgaattcc tcaaaaaagc
     1381 gtacagaaga ggaagataag gaattggccc tcatggagcg attggacgag gagtttggcg
     1441 ctgggcgcag cctcagggat atccagctcg agccggagga agctgcggga cttcgaggat
     1501 acggactgct gtcggtgaag cccattgtgg tagttgccaa catgagtgaa gagcaattca
     1561 gagaaggaaa gtttcctcga gacagtcaac ttcaagagta ttgttgcgaa cgaagcatac
     1621 ccctgatcag attggcggcg gagatagagg cggaaattgc ggagctcgat tctgacgaca
     1681 aagatctgtt catggctgct tacggaatag aggttagcgg tattgagcag gtatcgcgcg
     1741 cagtctatga tagcctggga cttgtctctt tcttcactgt cggtgaagat gaggttcgcg
     1801 cgtggccgat tgagaacggg accacagcaa agcgtgcggc ggggaagatt cattccgaca
     1861 ttgaacgagg ttttatccgg gcggaagtcg ttgcgtgcga cgacctcatg tcagccggat
     1921 ctctagccga tgccagatca gccgggagtg tgaggcttga gggcaaggaa tatgtgatgg
     1981 ccgatggcga cattgtcaac tttcgtttca gccctgccca ctagcatact gatggtccaa
     2041 cgaataccca tgccccagtc gcgggatgat acgtatagcg atacgtccgt tattccatcg
     2101 gaagggggtg cgggattggt tcaacagcaa gggctgacgc ctcgcgaggt cttgtatgct
     2161 caagaccagc tcagcaagga gcagctgtgt ttgaagaagt gtagcatcta ttcgaaccag
     2221 atccaagatc cgcagctgaa gggtagcgtt cagaggatga tggagtcatg ccaacgccat
     2281 gtcaatgccc tttcggggct tgtgggtcag tcaggacaga tgccaatgca atgatcttag
     2341 aatggtccga aaccgaaagg aggagaacta tggaccagaa caggctgagc gacagggata
     2401 ttgctgctga tctgttgagt gacctcaagg agttggcgct gggatatcac atggctacag
     2461 tggaggcgtc aaaccagagc gtgcgcgaaa ccttcaagaa cagtcatgat gactggatga
     2521 atgagcaacg cgggctctgg cagactatgt attcaaaagg ctggtatcaa ttacctcaga
     2581 ccggaccagg caagggaatg tagtctcaat ttcaaggcgg actgagtgtg acggaatcgg
     2641 gctgcattga tgtgtgcagt atctgaatat gtaaatggcg gccggagaaa tatccggccg
     2701 ccgattcact caggcgcata agggcttttc agatcgacgc gcaatcagtt gatggagacg
     2761 gagtgtgggt gaaggatggg catgaagcta atagcgtttg acatggacgg taccctgaca
     2821 caggaaccaa gctcgtggga gtacgtacat cgccgctttg ggctatggac aggccgtgct
     2881 gaggcgcata tgatgcgctt tctgtcaggg gaaatcacat atgaggagtt ttctatcctg
     2941 gatgcgcagg aatggaaggg gatgttgcat gcggagatcg agcgcacgct cggcgagatt
     3001 cactatgcta gatgtgcgca ggaggtagta gatgccgcac gatctcatgg agcattcacg
     3061 gctttgatat cgtctggcat cttagcgctt gctcgacgcg tcgccagtga tcttggcata
     3121 gatcgcgttt tcgcaaacga gctggtggca agggagggtg tgatgacagg tgatgtagtg
     3181 ataaatgttt caattgatga accgcatatg acaaaaggtg ctgtcttgag aaggctcaaa
     3241 atggagctcg gagccgatac agacgagacg tgggctgtcg gtgacaactg gggggacata
     3301 ggcatgttcc gtgaagcagg gactgccgta ttggtggcgc ctacacccga ggatagggag
     3361 aaggtcgtcg tggcagtccc gaacgtcgta tgtgtgccaa gcttatgcca tgtcgtcagt
     3421 cttatcagcc gaatcggagc tggcgattcc tgcagtcctg gcgcaaccgg cggctgaggt
     3481 gtcctgcata tccaattcgg gctctggcat gagttcgtcc ttgtattgga ggcggtaaag
     3541 atcatagtat agccctcgct tggcgaggag ttcctggtgg gtgccttcct ctcgaatgcg
     3601 gcctttatgg atcacgatga tcttgtcggc gtgttggatc gtcgacagtc tgtgggcgac
     3661 cacgatcgtt gtgcggcctg cgatcagctt tggcaaagcg tcttgtatga gcagctcagt
     3721 ctcggtatcg atatttgctg tcgcctcgtc gagaacgagt attgacggat cagaggcgag
     3781 ggctcgagcg aacgcaagca actgtctttg gccggcagac agtgtcgagc cgcgctcagt
     3841 gacgggctcg tcgtagccat tgggcaattt ggagatgaaa tggtcggcat ttacgtagcg
     3901 cgccacttct ttgatttttt catctgagat atccacgttg ttcagcctga tgttgctctt
     3961 tatgtctcca gcgaacagga acacgtcttg gagcactgct gcgatattgc gccgaaggtc
     4021 tgcgaccctg taactgcgaa tatcgactcc gtctatgagt atttgccctt tctggatatc
     4081 atagagccgg tttatcaagc ttgttattga ggttttgcct gcgccggtag cgccaacgaa
     4141 c
//
LOCUS       DAOSAI010000221         4139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13578_length_4139_cov_3.836680, whole genome shotgun sequence.
ACCESSION   DAOSAI010000221 DAOSAI010000000
VERSION     DAOSAI010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13578_length_4139_cov_3.836680"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLM83_07675"
     CDS             complement(<1..212)
                     /locus_tag="PLM83_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71318.1"
                     /translation="MNVQAVWVEIKKGLIRKIRSPVELITETLAAYVFLTVAIMAASG
                     RAVTSGDAADELLFSLACVFFCMGAL"
     gene            complement(686..1702)
                     /locus_tag="PLM83_07680"
     CDS             complement(686..1702)
                     /locus_tag="PLM83_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71319.1"
                     /translation="MSDLVVDMKSARKVFRRRRCDKPVVALCDLSLAIPKGEILGLLG
                     PNGAGKSTAIRMMSGLVTPTSGTILIGGYDVVEQRPEALSCVGVVLDRARTLYPRMTP
                     WENIQYFGIMKGLRDTERLRIRAGEWLDFFNIAEVADRPVNDLSMGTRQKVSLAVALA
                     AEPRVVLLDEPTVGLDVMSSLELRKAIKRMAHEEGCTVIMTTHQMDVAEDVCDRVCIL
                     NEGRVLALDTVRRLLDVFAFRAYTLTLAGSLTEEVRTALSGLGQVEVREKWLDGFGVS
                     HSGYTVIDIDVPDERGFFDVMGVLELAAVGVESVTRREPCLESVFLKLAGEEDGSFRQ
                     PSYA"
     gene            complement(2090..3334)
                     /gene="pepT"
                     /locus_tag="PLM83_07685"
     CDS             complement(2090..3334)
                     /gene="pepT"
                     /locus_tag="PLM83_07685"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOK71320.1"
                     /translation="MNKDALMQEGVAEKFMRYVRVNTESSDETDTTPSTEGQWDLARM
                     LAEELRALGLEDVAVDEHCFVIGRLPSNLDKPAPAVGFMAHLDTVPGIPGEGVKPVMH
                     RGYAGGEIEIGHGVILSPEDSPALAAAIGCDIITSDGSTLLGTDDKAGIAGIMQALVE
                     LIRNPDIPRPDVWIAFTPDEEIGKGVARFPYDKWGAKYAYTLDGGYLGELSEETFNAI
                     NGVVTIEGVSSHTGTARGKMVNAVHIAAELISAIPADWRPETTSGREGFTHPNEIEGN
                     VEKVSIKVIIRDFDAKLLEKRETAFIKTLEALEANYPGCSIKWAHTGGYRNMKEKLDE
                     DPRVVRLALEAMEMANVDPVVAPIRGGTDGSRLTCEGVLTPNLFAGGGDMHSRREWAC
                     VQWMEKAAEVVVNLCRLWAEEE"
     gene            complement(3349..3852)
                     /locus_tag="PLM83_07690"
     CDS             complement(3349..3852)
                     /locus_tag="PLM83_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71321.1"
                     /translation="MRALLVYHSRTGATERLIKSIGRALEGRGHRVEYHKLEAKRPMG
                     WFASSWAAFRKRETDLAEDMPKSLRMYELVLIAGPVIAGKTSAELNAFLNNMPDGIGR
                     RFGVFATSGSGAAEGPLRSMKSRIEHVGGFIIYEDVVKRQIAMDPEACTRRAEEIAEQ
                     LVRPWVW"
     gene            complement(3972..4097)
                     /locus_tag="PLM83_07695"
     CDS             complement(3972..4097)
                     /locus_tag="PLM83_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOK71322.1"
                     /translation="MHRDLPRTMKGAKVWGSTRFGGQFAPREWVLEDGDVVEFRT"
BASE COUNT          987 a         1290 c          908 g          954 t
ORIGIN      
        1 tgcaaggccc ccatacaaaa aaagacgcat gcaagggaaa acaggagctc atcggcagcg
       61 tccccggagg taacagcccg tcccgaggcg gccataatcg ccacagtcag aaagacatat
      121 gctgccaggg tctcagtaat cagttccaca ggacttctga tctttcggat cagtcccttc
      181 ttgatctcaa cccataccgc ttgcacattc atcagatatc acgctacgta aaccccctcc
      241 ttcgccttca attacagggg gaagtagcgt tttccctcct ctcgcacagc attggccgat
      301 gccgagatcc ttttgccatc caagttgcgc aggttaaaac agcctgtaac atgtatttcc
      361 aaagataaag cactcctagc ctttataccc aaccttgcaa aacaggccaa acccgtgaat
      421 atacccagct ctcctgcgct cgctgcttgg tgatgtaccc ataggcctcc gtaaacactt
      481 ggatataact cctgcagcat tgttcccatg cacgccacat cgtggcaaac cctttctgcg
      541 ccatccatgg taatgctggt tatcagctga atcattaagg gctccatgat tttgacgagt
      601 atccgcacgc ttcgtctctc acagcaagtt gcccatgaac tctttggcca tcacccgcaa
      661 tgtctaaggc gcatatgccc cagcttcagg cgtaggaagg ttggcgaaaa gagccatctt
      721 cttctccggc aagtttcaaa aacacagact ccagacaagg ttctcgcctg gttacagact
      781 cgacccccac tgctgccaac tccaacaccc ccataacatc gaagaatcca cgctcgtcag
      841 gaacatcaat atcgatcacc gtgtatccgg aatggcttac accgaagcca tccagccatt
      901 tttccctcac ctctacctgc cccaaaccgc tcaaagcggt tcgaacctct tcggtcaatg
      961 accctgctag tgtcaaggta taagctctga aagcgaagac gtccagaagg cgcctgactg
     1021 tatcgagagc caacaccctg ccttcattta agatacatac tctatcacat acatcctctg
     1081 ccacatccat ctgatgagta gtcatgatca cagtacaccc ttcttcatgg gccatgcgct
     1141 tgatcgcctt gcgcagttcc agagaggaca tgacatcaag gcccacagtg ggctcatcga
     1201 gaagaacaac ccgaggctct gccgcaaggg caaccgccag ggaaaccttt tgccttgtgc
     1261 ccatggatag gtcattcaca ggcctatcgg ccacttctgc aatgttgaaa aagtccagcc
     1321 attcgccggc gcgaattcga agcctctcag tatcccgcag acctttcatg atgccaaagt
     1381 attgtatgtt ctcccatggc gtcatccgag ggtaaagggt gcgtgcgcga tccaatacca
     1441 cacccacaca gctcaaagcc tcgggccttt gctccaccac atcataccct ccaataagta
     1501 tcgtgccgga tgtgggagta acaagccccg acatcatccg gatagcggtg ctcttccccg
     1561 ctccattggg accaagcagg cccaaaatct ctcccttggg aattgccaat gaaagatcgc
     1621 agagggcaac aacgggctta tcgcatcgcc gacgcctgaa taccttgcga gctgatttca
     1681 tgtcaacgac caaatcggac aagaataccg cctccctgcg gccatcacta cctgtaattg
     1741 taatatcata aatcaggata gtcaaccatt taatgggtta accatctggc tagtcccact
     1801 cgactagcat tgccataaag tcaggcgaag cgctgatatc ggctgaaaat ggcaagggct
     1861 agagaagaag cagtctcggg gttgatcgct ttacccgagc aacggtccgg atccgaaaaa
     1921 gcggcatcgt agcaaaagga ccctaacagg catgaaaaaa cccgtgcgct tccacacggg
     1981 taaccagaac gacacgattg gaatcaatat ggggagtcag gattacgcaa cttgccacag
     2041 gcaagcatgc ctcaacccac aaggtacatt ggaggaaact tgcagtcagc tattcctcct
     2101 ctgcccacaa cctgcacagg ttgactacta cttcagcagc cttctccatc cactgtacgc
     2161 acgcccactc gcgcctgctg tgcatgtcgc cgccgcctgc gaaaaggtta ggcgtgagga
     2221 cgccttcgca agtcagcctc gagccatcag tgccccctct tatgggcgca actacaggat
     2281 caacattggc catctccata gcttccaacg ctagcctcac gactcgggga tcttcatcca
     2341 atttttcctt catattgcga tagcccccgg tatgagccca cttgatgctg catccggggt
     2401 agttcgcctc cagcgcctcc aatgtcttga tgaaagcggt ctccctcttc tccagaagct
     2461 tggcatcgaa atcgcggatg atgactttta tcgatacctt ctccacattg ccttcgattt
     2521 cgttgggatg cgtaaagcct tccctgcctg acgtggtttc aggccgccag tcggcgggga
     2581 ttgcagaaat cagttcggcc gcaatgtgga ctgcattcac catctttccc ctagcagttc
     2641 cggtatggct cgacactccc tctatagtta caacaccatt tattgcgttg aaggtctcct
     2701 ccgaaagctc cccgagatat cccccgtcta gtgtgtaagc gtatttcgcg ccccacttat
     2761 catagggaaa tctggctacc cccttcccta tctcttcatc aggcgtaaag gctatccata
     2821 catcgggtcg agggatatca gggtttcgga tgagctcaac caaggcctgc attataccgg
     2881 cgatgccggc cttgtcatcc gtgcctagca aggtagatcc gtctgaggtg attatgtcac
     2941 agcctattgc agcagcaagt gctggcgaat cctctgggct caaaatgacg ccatgaccga
     3001 tctcaatctc tccgcccgca tatcctctat gcattacggg ttttacccct tctcccggaa
     3061 ttcccggaac agtgtcgaga tgagccatga aaccgactgc cggcgcaggc ttgtccagat
     3121 ttgacggcaa tctgcctata acaaaacagt gttcgtccac ggcaacatct tcaagcccaa
     3181 gtgcacgaag ttcttccgcc agcatgcggg cgagatccca ttgtccttcc gtgctaggag
     3241 tcgtatcggt ctcatccgaa ctctcggtgt tgaccctgac atatctcatg aacttctccg
     3301 cgacgccttc ttgcatcagt gcgtctttat tcattcatcg ccgccccctc accagaccca
     3361 cggcctaacc agctgctcgg caatctcctc cgcgcgtctg gtgcatgcct cgggatccat
     3421 cgctatctgc cttttcacaa catcctcgta aattatgaaa ccacccacat gttcaatcct
     3481 ggacttcatg gagcgaagcg gtccttcagc agccccactg cctgatgtcg caaaaactcc
     3541 aaaccggcgg ccgatgccgt caggcatgtt gttgagaaaa gcgttcagct ccgcagacgt
     3601 cttgcccgcg attacgggac ccgcaatcaa aaccaactca tacatgcgca agcttttcgg
     3661 catatcctcg gctaagtcgg tttccctctt tcggaacgca gcccaacttg aagcaaacca
     3721 ccccatcgga cgcttggcct cgagtttgtg atattccacg cggtggccac gcccttccaa
     3781 tgcccgtcct atagacttaa tcaatctctc agttgcacct gttcgtgaat gatatacaag
     3841 caaagctctc accggcagat cctccttctc gcagcatggc tctgtccgca aacactcctt
     3901 atagccaact ttaacctttt aacattcgct tcaggaatgc cttttccctt ctttgcggca
     3961 aagcatcagc atcaggttcg aaactcgacg acatcgccgt cttcaagcac ccattcgcgc
     4021 ggagcaaact ggccgccaaa ccgcgtagag ccccatactt tggcaccctt catggttcgt
     4081 ggcaaatcgc gatggatggc ttcggcccgg gccaggcgtg gtcgcctgtc ctcggcggt
//
LOCUS       DAOSAI010000222         4100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13758_length_4100_cov_3.176761, whole genome shotgun sequence.
ACCESSION   DAOSAI010000222 DAOSAI010000000
VERSION     DAOSAI010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13758_length_4100_cov_3.176761"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..500
                     /locus_tag="PLM83_07700"
     CDS             117..500
                     /locus_tag="PLM83_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71323.1"
                     /translation="MQQCVVHGSFLLDVGVLLALSIEAIDEIRDVESQADEIVRAAIE
                     EARARVAAAEERARESVVEAERSAMAEAARVEDESRARTAAEADKAKKQVESKVKELD
                     GIARSNWDKALKSAAGKVLSSIGDL"
     gene            487..2484
                     /locus_tag="PLM83_07705"
     CDS             487..2484
                     /locus_tag="PLM83_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOK71324.1"
                     /translation="MAISEMSRVTVLGHESRIGDVVDVLQQLGTAEVSQFGEDLADEE
                     LLDLACPYDASERIAELDSILAELSYTIDMMGKHRHIKRDIVQNFSGFRLYMTEDEWN
                     AYAEGGAEAARDIYSRAKAIEDTLAQLATNEASILTSIEILMPWQGLDAPIEEIRTGR
                     HVELVLGTADARKLEASLAEASEIVEGLAWELVGVDGERAYLLTAYPLSEAEEAARAL
                     SQAGFAEVRLDQYSGVPRDIIASLRKELDSIYRRRDQIEQEIDALVEDRPRVFVLHDH
                     FYLEREKYAITNRFGGTEHTFMVEGWVQTKDLEELRAEIEGIADDIVVLDRPRFEDEE
                     APVELSNKPLVAPFEVITKTMGYPKQGTIDPSPYLGPFFLVFFGLCMTDAVYGIFIAL
                     VAFQLMKKTHTRPGEGGFLDLIAMCGFATIFVGALLGGWLGDLPKRLFGWDTAILFDP
                     MESPTTFLILSFVMGIIHLYWGIIIKGIDNVKKGEWLSAIYDQGFWLVWLTSLMLTLG
                     ATALGPNGPAIAAVSKWFLIASAIGLIATQGRQHKNPIKRIGAGLISLYNTTGFMSNV
                     ISYARLFGLGFTSTVLASVMNDLFLRPAGIPVVGPVIAVVGLAFGHLFNILINIIGSY
                     VHSSRLQYVEFFGEFFEAGGRRFMPFGMSTKYVTVENSKEA"
     gene            2490..2969
                     /locus_tag="PLM83_07710"
     CDS             2490..2969
                     /locus_tag="PLM83_07710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOK71325.1"
                     /translation="MDFTLGTLLGFMAIGLAVIPGGYASSKAVGMVGRAGAGVITEDP
                     DKFGSILILQALPGTQGVYGFLTGLSIMVKIGVLGGSIQALTVNQGIMFILAAVPVAA
                     LGWLSAIFQGQVAASGVSIVAKRPEEMGKAVILAAMVETYAVLALLASLLMVLSINV"
     gene            2989..3594
                     /locus_tag="PLM83_07715"
     CDS             2989..3594
                     /locus_tag="PLM83_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOK71326.1"
                     /translation="MENLERLKGRILDEAREKADRILDAADERCAEIQREAEAKSDEI
                     RKRAVERARANAREEERRAEIMRSLDVRNAMLRAKGDAVDKLMAEIPATIRALPDEEY
                     LGIMKRMLINAAPAGQVELVVALPDRGRIGSDFVKEAASELLAAGKQTKLKLSTETAD
                     LTGGFILRASTVEVDCSLDSLLALCEDELAPLVAEALFGRS"
     gene            3597..>4100
                     /locus_tag="PLM83_07720"
     CDS             3597..>4100
                     /locus_tag="PLM83_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOK71327.1"
                     /translation="MGFDDRFAYVSGRIWALEDRMVDRAKFNRLIDAGSPEELVRILS
                     ETEYGQGREIVPERYEEIIDAELVRVHQLIAELSPDPELTGVFRAKHDFHNMKVHLKS
                     RLVKRDRDMIVREGLGLSQVGWIDPDRLGEYADAIMLEYYPVDEEEEDDPGERDDFVA
                     FDGSDEIL"
BASE COUNT          921 a          967 c         1235 g          977 t
ORIGIN      
        1 ccgtcaagat gatgtccccc ttgttcatgg gtgacattat ctcagacgcg taacattgtg
       61 acttcttcta tcgttgcacc agaccatgta tttgtagtat aatcccggat gagtccatgc
      121 aacaatgtgt agtgcatggg tcctttctgc tcgatgtggg ggtgttgttg gcattgagca
      181 tcgaggcgat agatgagatc cgtgatgttg aaagccaggc agacgagatc gtgcgcgctg
      241 cgattgagga agctagggct cgagtggccg cagcggagga gagagccaga gagtctgttg
      301 tagaggccga acgttcggcc atggctgagg cagccagggt agaggacgaa tcgcgcgctc
      361 gtacggcggc cgaggctgac aaggcgaaga agcaagttga gtccaaggtg aaggaattag
      421 acggtatagc gagatcaaac tgggacaagg ctctgaagtc ggccgctggg aaggtgttga
      481 gttctattgg cgatctctga gatgagcaga gtgaccgtgc tagggcatga gtcgcggatc
      541 ggagatgtcg tagacgtcct gcaacaactg ggcactgcag aggtgagcca gtttggtgag
      601 gaccttgctg acgaagaact cttggatctt gcatgcccct acgacgcttc tgaaagaatc
      661 gccgagctcg atagcattct ggcggagctc tcatacacca tcgacatgat gggtaagcat
      721 cgacacataa aacgggacat tgttcagaac ttttcaggtt tccgtctgta catgactgag
      781 gacgaatgga atgcttatgc tgaaggcggg gcggaagcgg ctcgcgatat ttacagccgg
      841 gccaaagcta ttgaagacac cctcgctcaa ctggcaacta atgaagcttc catcctgaca
      901 tcgattgaga tacttatgcc gtggcagggt ctggatgcgc cgatcgagga gatccgcacc
      961 ggacgccatg tagagctcgt gctggggact gccgatgcgc ggaaactcga ggcatccctc
     1021 gctgaagcct ccgagattgt ggaaggactc gcctgggagc tagtgggcgt cgacggtgag
     1081 cgagcttact tactgacggc atatcccctt tccgaggctg aagaggcggc tcgcgcgttg
     1141 tcgcaggcgg gttttgccga ggtaaggctg gatcagtatt ctggtgtccc gagggatatc
     1201 attgcaagcc tgcgaaagga attagacagc atttatcgaa gacgcgatca gatcgaacag
     1261 gagatcgatg ccctcgtgga ggaccgtccc cgcgtattcg tgcttcacga ccatttttac
     1321 cttgaacgtg agaaatacgc cattacaaac aggtttggcg gcactgaaca tacattcatg
     1381 gtcgaaggtt gggttcagac taaggacctc gaggagttgc gagccgagat tgaagggatc
     1441 gccgatgata tcgtggtatt ggacaggccg cggtttgagg atgaagaggc tccggtagag
     1501 ctttccaata agccacttgt ggctcctttt gaagtgataa ccaagaccat gggatacccc
     1561 aagcagggca ctatcgaccc cagcccatac ttgggcccat tctttcttgt tttcttcgga
     1621 ctgtgcatga cagatgccgt ctatggcatc tttattgcct tggtcgcctt ccaactcatg
     1681 aagaagaccc atacccgacc tggtgaaggt gggtttcttg acctcattgc catgtgcgga
     1741 tttgccacca tattcgtagg cgcgctgctc ggcggttggc tgggtgatct gccgaagcgt
     1801 ctttttggtt gggataccgc aatcctcttt gatccgatgg agagcccgac cacatttctc
     1861 attctctctt tcgtgatggg catcatccat ctgtattggg ggattatcat caagggtatt
     1921 gacaatgtga agaagggcga atggctatcc gccatatatg accagggatt ctggcttgtt
     1981 tggctgacga gcctgatgct gacccttggc gctacagccc tcggcccgaa cggccctgcg
     2041 atagcggctg tgtcaaaatg gtttctgatt gcctcagcca tagggctcat tgccactcag
     2101 ggccgccagc acaagaatcc cattaagaga ataggtgcag ggctgatatc gctctacaac
     2161 acgactgggt tcatgagcaa tgtcatatca tacgccaggc tatttggctt gggctttaca
     2221 agcactgtct tggcctctgt catgaacgat ctctttctga ggcccgcggg tattcccgta
     2281 gttggtccgg tcattgcggt agtggggttg gcattcggcc acctcttcaa tatcctcatc
     2341 aatatcattg gctcttacgt ccactccagc cgcctccagt acgtggagtt cttcggcgag
     2401 ttctttgagg ctggagggcg aagattcatg ccatttggca tgtcgacaaa gtacgtaacg
     2461 gtggaaaact ctaaggaggc atagcacaca tggatttcac actaggcaca cttctcgggt
     2521 tcatggcgat tggactcgcc gtaattcctg gcggttacgc atcttccaag gccgtcggaa
     2581 tggtgggacg agccggcgca ggagtcatta ccgaagatcc tgacaagttc ggttcgatcc
     2641 tgatcctcca ggcccttccc ggcactcagg gagtctacgg tttccttacg ggcctgtcga
     2701 tcatggtcaa gataggagta ttgggtggat caatccaggc gctcactgtt aatcagggga
     2761 tcatgttcat ccttgctgct gtccccgtag cggctttggg ttggctttcc gctatattcc
     2821 aagggcaggt cgccgcgtct ggcgttagca ttgttgcaaa gagaccggag gaaatgggaa
     2881 aagcggtaat cctagctgcc atggtggaga cgtacgcagt tttagcgctc ctcgcgagcc
     2941 tattgatggt actgtcgatc aacgtctgat cagccgggag tgtatattat ggaaaacctc
     3001 gaaagactga agggccgaat tctggatgag gcacgcgaaa aagcagatag gattctggac
     3061 gccgctgatg aaaggtgcgc tgagattcaa cgggaagccg aagcaaaatc ggatgaaata
     3121 aggaagcgtg ctgtcgaaag agcccgcgca aatgcgcgcg aggaggagag acgcgccgag
     3181 atcatgcgtt ctctcgatgt gcgcaatgct atgcttcgtg ccaagggcga tgccgttgac
     3241 aagctcatgg cggagattcc tgctacgata cgtgcgttgc cggatgaaga gtatttgggg
     3301 atcatgaaga ggatgttgat caatgcggcc ccggcaggcc aggtagaact ggttgttgcg
     3361 cttcctgacc gtgggcggat cggctctgac ttcgtgaaag aagcagcttc cgagttgctg
     3421 gcggcaggaa agcagaccaa acttaaactt tcgacggaaa cagcggatct cacaggcggg
     3481 ttcattcttc gagcatccac cgttgaggtg gattgttcct tagattcgtt gttggcgttg
     3541 tgcgaagacg agcttgcgcc ccttgttgca gaggctctat tcgggaggtc gtagctttgg
     3601 gtttcgatga caggtttgca tacgtatcgg gaaggatctg ggctcttgag gaccggatgg
     3661 tggacagagc caagttcaac cgcctgatag acgcaggcag ccctgaggag cttgttcgaa
     3721 ttctcagcga aactgagtat ggacagggcc gtgagatcgt tcctgaacgt tatgaggaga
     3781 tcatcgatgc ggagcttgtg cgcgtgcacc agcttattgc cgagctttcg cctgatccag
     3841 agttgactgg ggtattcaga gctaaacacg attttcacaa catgaaagtc catctcaagt
     3901 ctcggctggt caaacgggat cgggacatga tcgtccggga ggggcttggg ttgtcgcaag
     3961 tcggatggat cgaccctgat cggctcggag aatatgctga tgccataatg ctcgagtatt
     4021 atcctgttga tgaggaagag gaagatgatc cgggcgagcg cgacgatttc gtggctttcg
     4081 acgggtcaga tgagatctta
//
LOCUS       DAOSAI010000223         4068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_13915_length_4068_cov_3.699975, whole genome shotgun sequence.
ACCESSION   DAOSAI010000223 DAOSAI010000000
VERSION     DAOSAI010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13915_length_4068_cov_3.699975"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1129
                     /locus_tag="PLM83_07725"
     CDS             89..1129
                     /locus_tag="PLM83_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOK71328.1"
                     /translation="MRDRLGKGLMPILIVSLVVVSVLSLRIGAVELSGKEILAVIWDW
                     IVSTATGDCKGDSTPTEEIVLRIRMPRIVLAALVGASLALSGTVYQALFRNPMADPYV
                     IGASAGASLGATIAMMLPISVQTLHLGVVPLFAFIGSIASVATVFNLARVGERTPMVN
                     LILAGVAVGSILSSLVSLLMYLMPQDGLHGLMFWLMGGFSGRNWTHVLVMLPHLVLGL
                     TVIIYYWRELNAILLGEEEALSLGVDVGKVTRRLIAAASLLTAASVASSGIIGFVGLV
                     VPHLVRIVIGPDHKKLIPNACLLGAVFMLAADTVARSIIPPRELPVGIVTSLAGGPFF
                     IYLLRKYKVKEW"
     gene            1154..1855
                     /locus_tag="PLM83_07730"
                     /pseudo
     CDS             1154..1855
                     /locus_tag="PLM83_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032129.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
     gene            1980..2945
                     /gene="cbiB"
                     /locus_tag="PLM83_07735"
     CDS             1980..2945
                     /gene="cbiB"
                     /locus_tag="PLM83_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018752987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOK71329.1"
                     /translation="MSTGVMVVIAFLVDAAMGDPIRGTHPVVLMGRAIELLEKVLRPR
                     RAEPKTEKIMGAVLAATIVAGTYAVSRTLLNAVRGFCPHAGDILEIWLLSTALAARGL
                     CDSAMQVRAPLLDGNLPATRENLSMIVGRDTENLSEEEAVRATVETVAENTSDGVIAP
                     LLFGLIGGAPLALAYKAVNTLDSMVGYKDERYADFGWASARLDDIANFIPARIAGLFL
                     VAAAAVTGHDCRGAWRVMLRDQRAHPSPNSGVGEAAVAGALGVQLGGVNTYGEQESFR
                     PHIGDAVKTLEPRHIEESVHLMYWASAMAMAAGAALRAVIDGIGV"
     gene            2953..4056
                     /gene="cobD"
                     /locus_tag="PLM83_07740"
     CDS             2953..4056
                     /gene="cobD"
                     /locus_tag="PLM83_07740"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HOK71330.1"
                     /translation="MVDYCIPLHGGNVHRAARARRVDVEKVLDFSANINPLGPSPLAT
                     EAIRSNLHMIRHYPDPDCTELREELARYLQADAVSVIVGNGGAEIIQMLAYVLRPQKA
                     LILSPTFSEYGRAVACAGGTVVRVPLRRDDGYSIDYDMVRANMDGVDMVWVCNPNNPT
                     GTLLPRKAILELADIVARCGAALVVDEAFIDFVADSEEYSVRDEVQERRNLVVIGSLT
                     KFFAIPGLRVGYAIAPEDLADRLRRQAPPWNVNFLAQVAAVASLQDTEYMGSTRKFIA
                     RERSWLVRELEKLGAEVGVKVIPPSANFILLDMSESGLSSAKLCRRLSCSNILVRDCS
                     NFPFLSENHVRIAVRSRPENERLLEALREVFGE"
BASE COUNT          846 a          995 c         1288 g          939 t
ORIGIN      
        1 cgagggctct ctatcctgag ctgtttcggt aaggagctgg cctggcagcc cggcagatgg
       61 cgagtatagg aaatgcaggt gagggcgggt gcgggatagg ttgggcaaag gacttatgcc
      121 gattctgatt gtgtctttgg tagtagtgtc tgtcttgtca ttgagaatag gggcagtcga
      181 gctttccggg aaggagatac ttgctgtgat atgggactgg attgtctcga cggcaactgg
      241 tgactgcaaa ggagactcta ctcccaccga agagatcgtt cttaggatcc gaatgcccag
      301 gatcgtcctg gcggcgcttg tgggagcatc ccttgcgctg tctggcactg tgtatcaggc
      361 gctctttcgg aatccgatgg cagatccata cgtgatcggg gcgtcagccg gcgcatcctt
      421 aggggcgaca atagccatga tgctcccgat cagcgtccaa acgctccatc tgggagttgt
      481 tccgctcttc gccttcatcg gctctatagc cagcgttgct acggtgttca accttgcacg
      541 agtgggcgag aggactccaa tggtcaactt gattctcgcg ggcgttgctg ttggatctat
      601 tctctcgtcc ctcgtgtcgc ttcttatgta tctcatgccc caggacggcc ttcatggctt
      661 gatgttctgg ctcatggggg gcttttcggg gcgaaactgg acacacgttc tcgtgatgct
      721 tccccatctg gttctcggtc tgacagtgat catctactac tggagagagc taaatgcgat
      781 tcttttgggt gaggaggagg cactgagcct cggagtcgac gtcggcaaag tcacaagaag
      841 gctcatagcc gctgcctctc tgctaaccgc tgcttcagtg gcctccagcg ggatcatagg
      901 gttcgtgggc ttggtcgttc cacacttggt gagaatagtg atcggccctg accataagaa
      961 attgattcca aacgcatgtc tgctcggcgc agtattcatg ttggctgcag acaccgttgc
     1021 acgatctatc attcctccga gagagctgcc ggtgggaatc gtcacatcgc ttgcgggggg
     1081 gccgttcttt atctatttgt tgaggaaata caaggtgaag gagtggtgat cagatttgcc
     1141 tgcggccaca atcatgattc agggcactac atcctctgcg ggaaagagcg tgctttgcgc
     1201 cgggctttgc cgcctctttc atcaggaggg cttcagagtt gctcctttca aatcgcagaa
     1261 catgtccaca aagtccgcga ttacggctga cggcggcgaa atcagcgttt cccaggcttt
     1321 tcaggcgctt gcttgtggaa tccaaccgtc agcagacatg aatccagtac tgctcaaacc
     1381 cctcggcgac atgaccagcg aggcaatact tgctggcaag agcgttggcg tgatccacgc
     1441 ggcggaatac cggtacaggc acttaggtgt cgctctcggg tgcattgagc ggtcgtaccg
     1501 tcgactcgca gctgaatacg acattgtagt gatagagggc gccgggagcc ccgccgaggt
     1561 caacctgaag gacagggata tttgcaacat gaggatcgcc cggatggccg acgctccggt
     1621 tttactggtg gcgaatatcg atccaggcgg ggcgctggcc gccatcgtgg gcaccctcga
     1681 gctgctcgat gcggatgagc gggacagagt aaaggggctt gtgataaaca agttcagggg
     1741 agacttctcc cttcttgcgc cagggctcga cttcctcgaa aagaagacag gaaaaccggt
     1801 tttgggcgtg attccctaca tcgataacct tgccattgcg gaagaggact cgcttggcgg
     1861 aaaaagagga atctctttgg gggctatctc gacgctcgat gcggaatgcg acaggattgc
     1921 ggcggctgtg cggaaagcca tcgatatcga tgagctgttc gggattatgg ggctgtagga
     1981 tgagcacagg tgtgatggtc gtaatcgcct tcctcgttga tgcggcaatg ggggatccaa
     2041 tacgtggtac gcacccggta gttctcatgg gcagggcgat cgagttgctc gaaaaggttc
     2101 tccggcctag acgcgccgag cccaagacgg agaagatcat gggagcggtt cttgccgcta
     2161 caattgtagc aggaacctat gctgtctcca gaacgctgct caatgcagtg cgcggctttt
     2221 gtccgcacgc aggcgatatt ctggagatct ggctgttgtc gaccgccttg gcggctcgag
     2281 gcctctgtga tagcgccatg caggtgcgcg cgccgctgct agacggcaac cttccggcaa
     2341 ccagggagaa cttgagcatg atagtgggac gcgatacaga gaacctcagt gaggaagagg
     2401 cggttcgcgc aaccgtggag actgttgctg agaacacgtc tgatggggtg atagcgccgc
     2461 tcctgttcgg attgatagga ggcgcgccgt tggcattggc atataaggca gtcaacaccc
     2521 tcgactcgat ggtagggtac aaggatgaac gttatgccga tttcgggtgg gcgtctgcaa
     2581 gactggatga tattgcaaac ttcattcctg cccggattgc cggcctcttc ctggtggctg
     2641 cggccgcagt gacgggacat gactgtaggg gcgcatggcg tgtcatgttg agagaccaaa
     2701 gagcgcatcc aagcccaaac tcgggggtgg gagaggcagc ggttgcaggg gccttaggcg
     2761 tgcagctagg aggggtcaac acatatggag aacaggaatc tttcaggccg catatcggcg
     2821 atgctgtaaa gacccttgag cctcgccata ttgaagaatc tgtccacttg atgtactggg
     2881 cgtctgcaat ggcgatggcg gcaggggctg cattgcgcgc ggtcatcgat ggaatcgggg
     2941 tgtgagagtg taatggtgga ttattgtatc ccgcttcacg gcgggaatgt gcatcgtgcg
     3001 gcgcgcgccc ggcgcgtgga tgtggaaaag gtgctggatt tcagcgcaaa catcaatcct
     3061 ctcgggcctt cgccattggc gaccgaagcc ataaggtcca atctgcacat gataaggcat
     3121 tatccggatc cagactgcac tgagctgcga gaggagcttg ctcggtatct acaggcagat
     3181 gcggtgtcgg tgatagtggg aaacggcggg gcagagatta tccagatgct cgcgtacgtt
     3241 ctgaggcccc agaaggcgtt gatcctgtcg cccacattct ccgagtacgg cagagctgtg
     3301 gcatgtgcgg ggggaacggt tgtgagggtt ccgcttcgaa gagacgatgg atattccata
     3361 gactacgata tggtccgcgc gaacatggac ggcgtcgaca tggtgtgggt gtgcaatcct
     3421 aacaatccca cagggactct tttaccgagg aaagccatcc tggaactggc agacattgtg
     3481 gctcgatgcg gggctgcttt ggtggtagat gaggctttca tcgatttcgt tgctgattcg
     3541 gaagaataca gtgtgcggga cgaagttcaa gaaagaagga acctcgtggt tatcgggtct
     3601 ttgacgaagt tcttcgcaat ccccggactt cgagtcggct atgcgattgc ccccgaggac
     3661 ttggcagaca gattgcggcg gcaggcgccg ccatggaacg tgaactttct ggcccaggtt
     3721 gcagctgtgg cgtcattgca agatacggaa tacatggggt ccaccaggaa gtttattgcg
     3781 cgcgagcgtt cctggcttgt tcgagagctt gagaagctag gggcggaagt tggcgtcaag
     3841 gttatccctc cgtccgcaaa cttcatcctt cttgacatga gtgaatcggg gctgagctcg
     3901 gcaaagttgt gtcgtaggtt atcgtgctca aacattctgg tgcgcgattg cagcaatttc
     3961 ccattcctca gtgaaaacca tgtgcggatt gcagtgcgta gtcggcctga gaatgaaagg
     4021 ctccttgagg cgctcagaga ggtcttcggt gaatgagcag ctccaaga
//
LOCUS       DAOSAI010000224         4041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14043_length_4041_cov_2.680883, whole genome shotgun sequence.
ACCESSION   DAOSAI010000224 DAOSAI010000000
VERSION     DAOSAI010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14043_length_4041_cov_2.680883"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PLM83_07745"
     CDS             <1..881
                     /locus_tag="PLM83_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71331.1"
                     /translation="RVAAVVSGISYAQPAVTDNLALKFRKKLGDPFTIGVLHANVGGD
                     PGHENYAPCTLADLISTGIDYWALGHVHSPKVLSDKDPIVIYPGCTQGRNPRETGPGG
                     CFVVHVGAGGRVEYEFVPVDVIRWHVLDISIDGLACVGDLIEEAKAAIGGLARASDGR
                     SCAVRIRLLGRGPVHHQLVENGEAMDLVSAVVEPAMPGERFDWIESIQDFTKPEVDRK
                     ALLSGGSLISDFLALADEAGSDLSGLEGVKEVLVGDLAHSKAAQVIREMDAEGLMRIV
                     ERAESIGIDLLLGEEG"
     gene            885..3791
                     /locus_tag="PLM83_07750"
     CDS             885..3791
                     /locus_tag="PLM83_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK71332.1"
                     /translation="MRIREVHIDGYGVFRDQHIQNLGDGVALFVGPNEAGKSTLMSFI
                     RGVLFGFDRESQVGYCPPLAGGRHGGRLVVLMDDGEERVVQRYLERGQSRNPADVVGN
                     MSKALYSNVFAFSLSELASLESLGSEEVRARIYSAGAGLGTRTLFEARRVLLSNLDRL
                     YKRQGRVPEINALLREISDLENLIARLADKPAEYNELVRSMEQLVAERERIQEQLDEA
                     KFEHLWFMTLRATRSTWEQFYSARAILDSLEMEDDDGALARREAVMALAPKIRRLKLA
                     QESMVQLVDSIPGLEAVAMERRRSFEERLRELGGAWNEEAVESFDVSPGVVDEIRASR
                     KRISRQDGEVQRLAEAVRARREVADEAESRLARQRERLEEFGELSYATRDDIVARISE
                     IDSAEAILREVSVAEVRAEAAKGNMEAARIRVENATEEVEWRKEEVRRARSFASWLAP
                     AMVAAFSLFAAFGMDRGMGLSIGVAVLGILGAAAIVTSILNRAGDAGKKLEAAVEAAR
                     QADELFCCCRDEYVAASGEFARATRAYSSACLGLFGQEDIDGVRLVREREAARSDLAA
                     WDEMARLRRECEQAERDWERAQDEWRKAEEAHRVCLDELERLWEEWRTWMRARGIDPT
                     FGHDAAERTIDAVRDVARARQDMIDARMRLKDARERARRHAADVNEVARSLGLPGTDV
                     DGALVVVDELVAMVEQVEADQEKRARARAVVNELAAQFGSAFPSDDLQRAEECHQLHT
                     AQEIADLCDDAESRRQALESELKKLAEEQGRKAEKRRLMEHDDEIAIACLELESAKRR
                     LATQVEEWAAYRLCLELIDEACEKYERERQPQVLRDASEALARMTGGRWQSAIARVSG
                     LDSLDVVDDNGRALPHTALSCGTEQQLYLAVRCGLAREYCSHAQSMPVMIDEALVNFD
                     PERAEAAARVLADLADVTQILVFTCHPYMAECFRSTGRVTAQFELDGAEIRPLA"
     gene            complement(3851..>4041)
                     /locus_tag="PLM83_07755"
     CDS             complement(3851..>4041)
                     /locus_tag="PLM83_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71333.1"
                     /translation="TRFNTVPIPSPLILIASYGIIERSGFTQEKPICSVIVIATYTHT
                     CGKVWSLYRSCDVNVAPV"
BASE COUNT          817 a          903 c         1454 g          867 t
ORIGIN      
        1 gccgagtggc agccgtggtt tccgggatca gctatgcgca gcccgctgta accgacaacc
       61 tggccctcaa gttcaggaag aagctaggag atccgttcac aattggggtt ctccatgcga
      121 atgtgggagg cgacccgggg cacgaaaact atgctccgtg caccctggcg gacttgatct
      181 ctacaggcat tgactattgg gccctgggcc atgtgcattc tccaaaggta ctgtcggata
      241 aggatccgat tgtgatttac cccggctgta cccaggggag gaatccacgg gagactgggc
      301 ctggcggatg tttcgtggtg catgtgggcg ctggaggacg ggtggaatac gaatttgtcc
      361 ctgtggatgt gatacgttgg catgttctgg acatctctat agacggcttg gcttgtgtag
      421 gcgatttgat tgaggaggca aaggccgcta tcggcgggct tgcgcgggct tccgacggca
      481 ggtcttgtgc ggttcgcatc cgtctgctcg ggcgcggtcc ggtccaccat caacttgttg
      541 aaaacggcga ggctatggat cttgtgtcgg cggtcgtgga gcccgcgatg cccggagaac
      601 ggtttgattg gatcgaatcg atccaggatt tcaccaagcc tgaggtggac aggaaagcgc
      661 ttctttcggg gggaagcttg atttccgact ttttagctct ggcagatgag gctggctcgg
      721 atctttccgg gcttgaaggg gtaaaagaag ttttggtggg ggatctggct cactccaagg
      781 ctgcacaggt catcagagag atggatgctg aggggcttat gcgaattgtg gagagggccg
      841 agtcgattgg gatcgacctt cttttggggg aggaggggta ggggttgcgc atacgtgaag
      901 tccacataga cggttacggg gtctttcgtg atcagcacat ccaaaatctc ggcgatgggg
      961 tcgctctatt cgtcgggccc aacgaggcgg gcaagtccac cttgatgtca tttatccgcg
     1021 gggtgttatt tgggttcgat cgggagtctc aagtaggcta ttgtccgcct cttgcaggag
     1081 gccgccacgg cggaaggctt gtggtgctga tggacgatgg cgaggagagg gttgtgcagc
     1141 gttatcttga gcgtgggcag tcccgcaatc ccgccgatgt tgtgggcaac atgtcgaagg
     1201 ctctctactc caacgtgttc gcgttcagcc tgtctgagct ggcttcgttg gaatccctcg
     1261 gatctgaaga ggtgcgggcg aggatataca gcgctggggc gggcctcggc acgcgcaccc
     1321 ttttcgaggc gcgcagggtt ctcctctcca acctcgatcg gctctacaaa aggcaaggac
     1381 gggtgccgga gataaatgcg ttgctcaggg agatttcgga cttggagaac ctcattgcac
     1441 ggcttgcaga caagccggcg gaatacaacg agcttgttcg ttcaatggag cagttggtgg
     1501 cggagaggga gaggatccag gagcagttgg atgaagccaa attcgaacat ctgtggttca
     1561 tgaccctcag ggctacccgg tcgacgtggg aacagttcta ttcggcgagg gccatattag
     1621 acagcctcga gatggaagac gatgatggcg cattggccag gagggaggcg gttatggcgc
     1681 tggccccgaa gatacgccgc ctcaagctgg cacaggagag catggtccag ctggtagact
     1741 ccattcctgg gcttgaagct gtggcgatgg agcggaggcg atcgttcgaa gaacgcttga
     1801 gggagctggg cggggcgtgg aatgaagagg ctgtggagtc tttcgatgtg tctcctggcg
     1861 tggttgatga gattcgggct tcacgtaagc ggatttcgag gcaggatgga gaagtgcagc
     1921 gccttgcgga ggcggttcgg gctaggcgcg aggttgcgga tgaggccgaa tcgaggctgg
     1981 ccagacagag ggagcggctt gaagaattcg gggagttgag ttacgcgacc agagacgaca
     2041 ttgtggcgcg gatcagcgag attgacagcg cagaagcgat cttgagggag gtcagcgttg
     2101 ccgaggtgag ggctgaggct gccaaaggga acatggaagc cgcgaggatc cgcgtggaaa
     2161 acgcgacgga ggaggtggag tggcgtaagg aagaagttcg ccgggcgcga agcttcgcct
     2221 cctggttggc ccctgccatg gttgcagctt tttccctttt tgccgctttc ggaatggaca
     2281 ggggaatggg tctgtccatt ggagtcgcgg ttttgggaat tctcggtgcg gctgcgattg
     2341 tcacgtcgat tctgaatagg gcgggggacg ccggtaagaa gttggaagcg gctgtggaag
     2401 cggcgaggca agccgatgag cttttctgtt gctgccggga tgagtatgtt gctgcatcgg
     2461 gcgagttcgc gagggctact cgcgcatatt cctctgcgtg tttggggctt ttcggccagg
     2521 aggatataga cggggtcagg ctggtgcggg agagggaggc ggccaggagc gacttggcag
     2581 catgggatga aatggctcgg ttgcggcgcg agtgcgaaca ggcggagagg gattgggagc
     2641 gggcgcagga cgaatggagg aaggctgaag aggcgcacag ggtgtgcctg gatgaactgg
     2701 agcgtctgtg ggaggaatgg cgaacctgga tgagggcgcg ggggattgat cccacctttg
     2761 gccatgatgc ggcagagagg acgatagatg ctgttcgcga tgttgcgcgg gcgcggcaag
     2821 acatgatcga tgccaggatg aggctgaagg atgcgaggga acgcgccagg aggcatgcgg
     2881 ctgatgtgaa cgaggtcgcg agatcgctgg ggctgccggg gacggatgtg gatggtgcat
     2941 tggttgttgt agacgagctc gttgcaatgg tggaacaggt cgaggcggat caggagaaga
     3001 gggcgcgggc gcgagcggtg gtgaacgagc ttgctgcgca attcggttcg gcttttccct
     3061 ccgatgatct ccagcgtgct gaggaatgtc atcagttgca tacggcccaa gagatagccg
     3121 acttgtgcga tgatgccgaa agccgccgtc aggctttgga gtcggaactg aagaagcttg
     3181 cggaagagca ggggcgaaag gctgaaaaga ggcggctcat ggagcatgat gacgagatcg
     3241 ccatcgcctg tttagagctg gagtcggcca agagaaggct tgcgacgcag gtagaggaat
     3301 gggcggctta taggctatgc cttgaattga tcgatgaggc gtgtgaaaag tatgagcgag
     3361 aaaggcagcc acaggtgctt cgcgacgcgt cggaagctct cgcccggatg accgggggac
     3421 ggtggcagtc tgccatagcg cgggtatcgg gcctcgattc cctcgatgtg gtggatgaca
     3481 atggcagggc attgccccat actgcactta gctgcggaac tgagcagcag ctctacttgg
     3541 cggtcaggtg cggcctggct agggaatact gctcccatgc tcaatcgatg ccagtgatga
     3601 tcgatgaggc gcttgtcaat ttcgatcccg agcgagctga ggcggctgca cgtgttctgg
     3661 cggatcttgc agatgtcact cagattctgg tcttcacgtg tcatccatac atggcagaat
     3721 gcttcaggag cacgggcagg gtgacggccc aattcgagct ggatggtgcc gagatcaggc
     3781 cgcttgcctg aggtttcggt ctgcttaggg gactgctatt tgtcccttgg tccgacccca
     3841 acatgccttg tcagactgga gctacattca catcacatga tcggtacaaa gaccaaacct
     3901 tgccacaggt gtgcgtatat gtggcaatca ctatcactga gcagatgggt ttctcctggg
     3961 tgaagccgct tctttcgatg atgccatagg aggcaatgag tattaatggg gaagggatgg
     4021 ggacagtgtt gaaacgggta t
//
LOCUS       DAOSAI010000225         4038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14053_length_4038_cov_3.712779, whole genome shotgun sequence.
ACCESSION   DAOSAI010000225 DAOSAI010000000
VERSION     DAOSAI010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14053_length_4038_cov_3.712779"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLM83_07760"
     CDS             <1..550
                     /locus_tag="PLM83_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK71334.1"
                     /translation="AYEQEVLSRLNDSLMAMHPDTLDDMPWMARKWDVGVWEPEAGKK
                     GTTVTWYIEKGIKWSDGMPFTAEDVEFTINYLKANKVPRYLPATQDIAKVELIDKYTV
                     KVYFKNVSYWHIYSADLSFLPKHIWKDVKDWKGFEPWKEPHPKIKGYSKLVGTGPFIL
                     KEYVPGEYVRLVKNPNYWRLKK"
     gene            640..1623
                     /locus_tag="PLM83_07765"
     CDS             640..1623
                     /locus_tag="PLM83_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71335.1"
                     /translation="MGFRSYVIKRLVYALIVLYVILTLNFVLFRVMPGDPTKMIIDPN
                     FTPEAKAELRRQYGLDDPPLKQYFTYMGNLLKFDMGLSFSTRRPVWSELKERLPNTIK
                     LFLCTFILTSLTGMRLGVKAAANPGSFTDRVVITAGLFTNAVPTFFIQLLMLLLFGYY
                     WPILPIHGTMSAPPPAGAWNVFLDRLWHLVLPVTSNMIVGFGSWALYARNTMTEALGQ
                     DYIVTARAKGISEKDVLYHHALRSVLPPIVTLLFMSLPGMVSGAVITESIFSWHGVGR
                     YLLDATLQMDYPAAQGAFYLIALAVVISNLLADIAYGLVDPRIRVGAGGAK"
     gene            1620..3287
                     /locus_tag="PLM83_07770"
     CDS             1620..3287
                     /locus_tag="PLM83_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71336.1"
                     /translation="MSQQTEPRSKSEEKAAAVKAATVAVPKPLTFWEQYRRKPQGMVG
                     LGIVIVYVLVAIFAPYIAPYDPMEDLYLADRLAAPAWMAKTSAKLKDAPPTIRMNLGP
                     DDWVREEVDDISISPYEFQYFDGVGMEIVLPQTHVVEVEETVEEDTWEEEGGFWASAA
                     IDWDPWGLLESDEETEPSPEPEDEYGFDFEPEIDSGIDASAKKLLLTYEVDYDYTPPQ
                     TFSFSFDYSVDAPSSAETVIMLELMTPDGEVFDVWDAKVYGSVESGTALVDARDYDLK
                     MRLGQGFFDEPTDILFSERGTYTLRLSSWTDSKEGPVTLKVTPVKFEILGLVHGILGV
                     DHMGSDLWSQLIYGTRIALAIGLSSAFIAIVIGTSVGLCAGYFGGAVDEVLMRIADVL
                     MSIPDLPVLIILGALFGKSVLNIVILLAIFSWMGIARLVRSQTLSLKERMFVEAAKAS
                     GASGGYIMREHILPNVMPLIFANLVLRIPGAILTEASLSFLGLGDPRVPTWGRMLQNA
                     KQFGAFTRLAWWWLLPPGIALTLLSLAFVFIGNSVNEIYNPRYRERS"
     gene            3290..>4038
                     /locus_tag="PLM83_07775"
     CDS             3290..>4038
                     /locus_tag="PLM83_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71337.1"
                     /translation="MALVEVRDLKMYYRTLRGHVKAVDSVSFDIERGKAVGIAGESGC
                     GKSSMAISILRLLPRNGEYHGGSVVMNGQDILKMPEEQFRKDVRWKQISMIFQGAMNA
                     LNPVHRVGDQIVEAILEHEDVSHEEAVSRTKDLFDLVGINPNRFGEYPHEFSGGMKQR
                     AVIAMALACHPDLIIADEPTTALDVMVQAQILKAIGELREELNLSMMLITHDLSVIAQ
                     TCDSVVIMYAGKVAEYGSVMDVYVNPTHPYA"
BASE COUNT          915 a          952 c         1174 g          997 t
ORIGIN      
        1 cgcctacgag caggaagtgc tctcaaggtt gaatgacagc ctgatggcaa tgcatcctga
       61 cactctcgat gacatgcctt ggatggcccg taagtgggac gtcggcgtct gggagcctga
      121 ggcgggcaaa aagggcacca cggtgacctg gtatatcgag aaggggatca agtggtctga
      181 cggaatgccg ttcaccgctg aggatgtaga attcaccatc aactacctca aagcgaataa
      241 ggttccgagg tatctccctg cgacgcagga cattgccaag gttgagctga tcgacaagta
      301 taccgtgaag gtttacttca aaaacgtcag ctactggcac atctacagtg cagacctgtc
      361 cttcctgcct aagcacatct ggaaggatgt aaaggactgg aagggcttcg agccttggaa
      421 ggagccgcac ccgaagatta agggttactc caaacttgtt ggaacgggac ccttcattct
      481 taaggaatac gtgcccggcg aatatgtaag gctcgttaag aacccgaact actggcgtct
      541 gaagaagtag ggtctaaatg atctggcttt ttgccgcccc gggccattgg ggcctggggc
      601 ggcatagccg gtgataaggc agaatgaggt gagacggatt tgggttttcg ttcgtatgtc
      661 atcaagcgat tggtctatgc cttaatagtc ctatacgtca tactcaccct caacttcgtc
      721 ctcttccgtg tgatgccggg tgacccgact aagatgatca ttgatcccaa tttcacaccg
      781 gaagcgaagg ctgaattgag gagacagtac ggcttagatg atcccccatt aaagcagtac
      841 tttacctata tggggaattt gctcaaattt gatatgggcc tttctttttc gacgagacgg
      901 ccagtctggt cggaactcaa ggaaaggctt cccaatacga tcaagctgtt cctttgcaca
      961 ttcatcctca catcattgac ggggatgcgc ttgggagtga aggccgctgc aaatccaggc
     1021 tcgtttacgg atcgagtcgt gataacagca ggtcttttta cgaatgcagt gcctacgttt
     1081 ttcatccagt tgctcatgct tctcctattc ggttactatt ggcccatatt gcccatacac
     1141 ggcactatga gcgcgcctcc tccggctgga gcatggaatg ttttccttga taggctatgg
     1201 cacctggtac tcccggtaac gagcaacatg attgtgggat ttggttcatg ggctctgtat
     1261 gctaggaaca caatgaccga agcgcttggg caggactaca tcgtaactgc gcgggccaag
     1321 gggatttcgg agaaagatgt cctttatcac catgcgttgc gcagcgttct cccgccgata
     1381 gttaccttgc tcttcatgtc gttgcctggg atggtgtcag gggctgttat cacggagagc
     1441 atattctcat ggcatggggt tggaaggtac cttctcgatg ctacgcttca gatggactat
     1501 ccggcggcgc aaggcgcttt ctatcttata gccttggccg ttgtgataag caatttactg
     1561 gcggatattg cctacggtct tgttgatccg aggatccgcg ttggggcagg tggtgcaaag
     1621 tgagtcagca gactgagcca aggtcgaaaa gtgaggaaaa agctgctgct gttaaggctg
     1681 ccaccgtcgc tgttccaaag ccgcttacct tttgggaaca gtacaggcgc aagccccagg
     1741 gtatggtggg cctcggcata gttatcgtat acgttctggt tgcgattttc gcaccgtata
     1801 tcgcgcctta cgatccgatg gaagatttgt atcttgctga taggctggct gcacccgctt
     1861 ggatggccaa gacgagcgca aaactcaagg acgctccgcc gaccatacgc atgaacctcg
     1921 gcccggatga ttgggtccgc gaagaggtgg atgacatatc catttccccg tacgagtttc
     1981 aatactttga cggggttgga atggagatag tcctgccgca gactcatgtg gtggaggtag
     2041 aagagaccgt cgaggaggat acgtgggaag aagagggggg cttttgggcc tcagctgcta
     2101 tcgattggga tccgtgggga cttttggaaa gcgatgagga gactgagcct tcgccagagc
     2161 ccgaggatga atatggtttc gacttcgaac cggagattga ctcagggatc gatgcatccg
     2221 ctaaaaagct gctcttgacg tatgaggttg attatgacta tactcctccg cagacatttt
     2281 cgttcagttt cgattactca gttgatgcgc ctagcagtgc cgaaactgtg atcatgctgg
     2341 aactgatgac cccggacgga gaggtcttcg atgtttggga tgcaaaggtc tacggaagtg
     2401 tggagtcagg aacggccctc gttgatgcga gagactatga tttaaagatg cgactgggcc
     2461 agggcttctt cgacgagcca actgatatcc tcttctctga gaggggcact tacaccttga
     2521 ggctcagctc gtggacggac tcgaaggagg gccctgtcac actcaaggtt accccggtga
     2581 aatttgagat tctcggtttg gttcatggaa ttctcggggt tgaccatatg ggttcagacc
     2641 tctggtcgca gctgatctat ggaacgcgaa ttgcgcttgc aattggtctg tcatccgcgt
     2701 ttatcgcgat cgtgatagga actagtgtgg gcctttgtgc ggggtatttt gggggcgcag
     2761 tggatgaagt cctcatgcga atcgcggacg tgctcatgtc catccccgac ttgccggttc
     2821 tgattatcct cggcgctcta tttggcaaga gtgtccttaa catagtcatc ttgcttgcta
     2881 tcttctcatg gatgggaatt gcaaggctgg tgagatcgca gacactgtcg ctgaaggaac
     2941 ggatgtttgt agaggcggct aaggcatcag gggcatccgg tggttacatc atgcgcgagc
     3001 acatactgcc gaacgtgatg ccgctgatct ttgccaatct ggtcctcagg atacctggag
     3061 caattctcac ggaggcttca ttgagcttcc ttggcctggg agacccgcgc gttcctacat
     3121 ggggaagaat gctgcagaat gccaagcagt ttggtgcctt tacgcgtctg gcttggtggt
     3181 ggctgctccc gcccggtata gcgcttacgc ttctaagctt ggcgtttgtg tttatcggga
     3241 actcggttaa cgagatctac aatccacggt atagggagag atcgtaacaa tggccctggt
     3301 tgaagtgaga gacctgaaaa tgtactaccg gaccctcagg ggccatgtta aggcggtcga
     3361 ttccgtaagc tttgacattg agcgtggaaa ggcagtggga atcgctgggg aatccgggtg
     3421 tggcaaatcg agcatggcga tctcaatcct ccgcctgttg ccaaggaacg gtgagtatca
     3481 cggcggcagt gtggttatga acgggcagga tatactgaag atgcctgagg agcagtttcg
     3541 caaagatgtt cgatggaagc agatatcaat gatatttcag ggcgcaatga atgccctgaa
     3601 cccagtccac agggtcggcg atcagatcgt cgaggctatc ctcgaacatg aggatgtatc
     3661 ccatgaagag gctgtgagcc ggacaaaaga cctgttcgac cttgttggca ttaacccgaa
     3721 cagatttggg gagtatccgc acgagttcag tgggggcatg aagcagcggg cggtcatcgc
     3781 catggccctt gcatgtcacc ctgacctcat tatcgcggat gagcccacaa cggcgctgga
     3841 cgtgatggtt caggcgcaga tcctgaaggc tatcggagaa ctgagagagg aactcaacct
     3901 ctctatgatg ttgatcactc acgacctgtc ggtgattgcg cagacctgcg actctgtcgt
     3961 tatcatgtat gccggcaaag ttgcggagta cggcagcgtc atggatgttt acgtgaatcc
     4021 aactcaccca tatgccat
//
LOCUS       DAOSAI010000226         4016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14143_length_4016_cov_5.150720, whole genome shotgun sequence.
ACCESSION   DAOSAI010000226 DAOSAI010000000
VERSION     DAOSAI010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14143_length_4016_cov_5.150720"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLM83_07780"
     CDS             <1..632
                     /locus_tag="PLM83_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71338.1"
                     /translation="ECPFKIGRKPNNRTKFAFLFLAVFSIATAICPPALCQKVQSQVV
                     GDLTLAVATPVGVDLDIAYADSFSFRIPLNAGYLIYERADSQGVRHLCLSDYNQSGID
                     FELIVDLFPIDAQSPTSLDALARGLLYAGARATAIDSKVVEISGRPGFDLTVKGIVYD
                     RIVPQDEIRMVFLTDFDSGLTFTLIEHQHRPEDHANMLDKILSTLIFTD"
     gene            805..1050
                     /locus_tag="PLM83_07785"
     CDS             805..1050
                     /locus_tag="PLM83_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOK71339.1"
                     /translation="MKSTGIVRKVDELGRVVIPIELRRTLQINEKDSLEIYVDGEKII
                     LKKYEPACIFCGNADGIRIFKDKNICKDCFDLMKSVG"
     gene            complement(1126..2037)
                     /gene="rsmI"
                     /locus_tag="PLM83_07790"
     CDS             complement(1126..2037)
                     /gene="rsmI"
                     /locus_tag="PLM83_07790"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOK71340.1"
                     /translation="MTRYGEAAEKGTLYLVATPIGNMDDITDRARAVLAEADIVAAED
                     TRHTGLLLHRLGIDARLLSCHEHNEAARVEQIIAMLGRGEDVAVVSDAGMPGISDPGE
                     IIVAAAVEGGFKVVPVPGPTAFVAALTASGLPTSRFFFQGFLPRKVGDRRKVLEELSS
                     LPVTLIFYEAPHRLERMLEDVADILGDRRCVVARELTKKFEDIQRGTVGETLEWVRER
                     KPKGEIVVIVEGCSQRSAATEGCPEGNAEDELEMAVAEVAQLVGEGFTMRDAVSKVAE
                     ERGISRREVYARAHEERDGGRKKQKGT"
     gene            complement(2088..2873)
                     /locus_tag="PLM83_07795"
     CDS             complement(2088..2873)
                     /locus_tag="PLM83_07795"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOK71341.1"
                     /translation="MSSGVRLMSGGRADETREALLHDYERLDDLQRSGLVIIQNPAGF
                     RFSMDAVLLAEFATVRPRDRAIDLGTGTGVIPLLVWARRQPAEIVGIEILEEMADMAK
                     RSVQINGLSDRIKIVHGDLRDAVDLFGAGYFDVVLSNPPYIKVDEGTLSPEGHLTVAK
                     HEVSCTLEDVVKVAAGLVKPRGRVAMIHRASRLADLLSSMREANLEPKRLRLVQARVG
                     APPMMVLVEAVKDVKAGLQVMPTLMLYGEDGQYSEETKQIYFG"
     gene            complement(2982..3797)
                     /locus_tag="PLM83_07800"
     CDS             complement(2982..3797)
                     /locus_tag="PLM83_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOK71342.1"
                     /translation="MVKIVGVRFKKAGKVYYFDPGELDLAAGDDVIVETARGVEFGQV
                     VIGPKFVDEEEIVQPLKQVIRGATDADRRQVEENKKKAERAFDIGLEKIAAHGLPMKL
                     VDVEYTFDNSKIVFYFTADGRVDFRELVRDLASVFRTRIELRQIGVRDEAKMLGGIGS
                     CGRALCCASFLGEFEPVSIRMAKEQNLSLNPSKISGICGRLMCCLKYEINPVRSETED
                     MPELGDRVITPSGPGKVMSIDPETRSVRVQLDSGGHASISLTELEYECADKEE"
     gene            complement(3813..>4016)
                     /locus_tag="PLM83_07805"
     CDS             complement(3813..>4016)
                     /locus_tag="PLM83_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71343.1"
                     /translation="ILSITGDESLMINIDRIMEIREDVRKGEPCLFSRWTVKAMQTAQ
                     ALRSSANPRLAMDDFFLNLALPE"
BASE COUNT          936 a         1222 c          905 g          953 t
ORIGIN      
        1 gagaatgtcc attcaaaatc ggtcgcaaac cgaacaatcg cacgaaattt gcgtttctct
       61 tcttggccgt gttcagcatt gcaacagcga tctgccctcc cgccctctgc caaaaggtgc
      121 agtctcaagt tgttggtgac ctaacgctgg ccgtcgcaac gcctgttggg gttgatctcg
      181 acattgccta tgccgatagc ttctccttca gaatcccatt aaatgccgga tacctgatct
      241 acgagagggc agatagccaa ggcgtccgtc atctatgcct ttccgattac aaccagagcg
      301 ggatcgactt cgagctcatc gtcgacctct ttccaattga tgcccaatca cctacatcat
      361 tggacgctct tgcaagaggc ttgctttacg cgggagcaag agcaacggca atcgattcta
      421 aagtcgtgga gatatcgggg cgccccggat ttgatctcac cgtcaaggga atcgtctacg
      481 accgaatcgt gccgcaagat gagattcgga tggtctttct tacagatttt gattcgggac
      541 tcacgtttac cctcatagag catcagcacc gtccggaaga tcatgcaaat atgttggata
      601 aaatcttatc taccctgata ttcactgatt aacggagtaa aaaatgcttt tgttaggcaa
      661 aagtgcacgg aatatgactg ttttgcccaa atcggcagcg taaaacccaa gaatttgtga
      721 aattagtact tgactcctct tggaaaagat actattattt gtttggcagg cattgccgag
      781 tatttgcgtg ggagggtcgc gaaaatgaaa tctaccggta tagttagaaa agtcgacgaa
      841 ctaggaagag tcgtaatacc catcgaacta cgaagaacgt tgcagatcaa tgagaaggat
      901 tccttggaaa tctatgttga cggggaaaag atcatcctga agaagtacga acctgcctgc
      961 atattctgtg gcaacgctga tggaatcagg atcttcaagg acaagaatat ctgcaaggat
     1021 tgcttcgacc tgatgaaaag cgtaggctag tcggttctcg acttgcctcg ccccatccgg
     1081 gcgaaagcac tgcaaatagc aacgttcgtc tggcaaaaag gcactttaag tgcctttttg
     1141 ctttttccga ccgccatccc gctcctcatg tgcacgcgca tatacctcgc gccttgagat
     1201 gccacgctcc tctgccacct tggaaacagc atccctcatt gtaaaaccct cgcccacgag
     1261 ctgcgccacc tcggccacag ccatttccaa ctcgtcctcg gcgttccctt ccgggcaacc
     1321 ctctgtcgcc gcacttcgtt ggctgcaccc ttcgactatg actacaatct ctcccttggg
     1381 cttgcgttcc ctcacccact ccagcgtctc gcctacggtg cctctctgta tgtcctcgaa
     1441 tttcttagta agttctcgcg ccaccacaca tcttctgtcg ccgagaatgt cagccacatc
     1501 ttcaagcatg cgctcgagtc gatgcggcgc ttcgtagaaa atgagagtga ccggcaatga
     1561 agaaagctct tcaagaactt tacgcctatc ccccaccttc ctaggaagaa agccttgaaa
     1621 aaagaatctg cttgtcggca atccagatgc agtcaacgct gcaacgaacg ccgtggggcc
     1681 cggaacaggt accactttga acccgccctc caccgcagca gcaacaatga tttctcccgg
     1741 atcagagatt cccggcatcc ctgcatcaga gaccacggcc acatcttcgc ccctgcccag
     1801 catcgcaatg atctgctcca cccgagccgc ttcgttgtgt tcatggcacg acagcaacct
     1861 tgcgtcaatt cccaggcgat gcaacaagag cccggtatgc ctggtatcct cagcggccac
     1921 gatatccgct tcggccagaa cagccctggc tctgtcggta atgtcatcca tgttcccaat
     1981 aggcgtggcc accaagtaaa gagtaccctt ttcggcagct tcgccgtagc gtgtcaccgg
     2041 cgatcaccct ccaccaactc tacatgtccc caagtttcgc ccgtctctta gccgaagtat
     2101 atctgttttg tttcttcaga atactgcccg tcctctccat aaagcatcaa agtgggcatt
     2161 acctgcagcc cagccttaac atccttcacc gcttcgacca ataccatcat gggcggcgcg
     2221 cccaccctgg cctgcaccag ccggagacgc ttcggttcca gatttgcctc gcgcatggat
     2281 gaaagaagat ccgcaagcct ggatgcacgg tgaatcatag cgactctacc ccttggcttc
     2341 acaagtcctg cagccacttt caccacgtct tccagagtgc acgacacttc atgcttggcg
     2401 actgttaggt gcccttcagg gctcagagtt ccctcatcaa ccttgatata tgggggattt
     2461 gatagcacaa catcgaaata gcctgcgcca aaaaggtcta cagcgtccct caagtcgcca
     2521 tggacgatct ttattctatc ggaaagcccg ttgatctgga cacttcgctt cgccatgtcg
     2581 gccatctctt ccagaatttc aatgccgaca atctccgccg gctgtcgcct ggcccagacc
     2641 aggaggggaa tcacgcctgt gcctgtgcca agatctatag ccctgtcccg cggcctaaca
     2701 gtcgcgaatt ccgcaaggag cacggcatcc atggaaaacc tgaacccggc aggattctga
     2761 attatcacaa gtccggaccg ttgcaggtcg tccaatctct cgtagtcatg cagcagcgcc
     2821 tcgcgcgttt catccgccct cccgccactc ataagccgta cgcctgacga caatcatgca
     2881 atctgcccgc gcatgattgg gtcgtgcgcg atatccccct ttcaccaagt aacatgcaca
     2941 ccgcctaaag caccgggttt gcctcagagc cgacggcggc gtcactcctc cttatccgca
     3001 cattcgtatt caagttctgt cagagagatc gacgcatgcc ccccactatc cagttgaacc
     3061 ctgacagaac gcgtctcagg gtctatcgac attaccttgc ccgggcccga aggagtgatg
     3121 actctatcgc ccagttccgg catgtcctca gtttcggacc ttacaggatt tatctcatac
     3181 ttgagacaac acatgagcct tccgcagatt ccagaaatct tggagggatt aagcgacaga
     3241 ttctgctctt ttgccatgcg tatcgataca ggctcaaact ccccaaggaa agaggcacag
     3301 caaagcgcgc gtccacacga cccgatccct ccaagcatct tggcctcatc cctcacccct
     3361 atctgcctca gttcgatgcg ggttcggaag acagaagcca gatccctgac gagctccctg
     3421 aagtcgacac gtccgtcagc agtaaagtag aagactatct ttgagttgtc gaacgtgtat
     3481 tcaacatcca caagcttcat gggaaggcca tgcgccgcaa tcttttcgag cccaatatcg
     3541 aaggctcgtt ccgctttttt cttgttctcc tcaacctggc gcctatcagc gtcagtcgcg
     3601 ccccttatca cctgcttcag gggctgtact atctcttcct catccacaaa cttgggcccg
     3661 atcaccacct ggccgaattc gacgccgcgg gcagtctcga caatgacgtc atcacctgcc
     3721 gcaagatcga gctctccggg gtcgaagtaa tatacctttc ccgctttctt gaaccgcact
     3781 ccaactatct taaccatgtt gcggacaccc tttcattcag gcaaggccag gttcaagaag
     3841 aagtcatcca tggctagccg cggattcgcg ctgctcctga gcgcttgtgc agtctgcatc
     3901 gctttgactg tccatcgact gaacaagcag ggctcgcctt tgcgcacatc ttcgcgaatc
     3961 tccatgatgc gatcgatgtt tatcatcaga ctctcgtcac ctgttatgga caggat
//
LOCUS       DAOSAI010000227         4016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14145_length_4016_cov_3.424135, whole genome shotgun sequence.
ACCESSION   DAOSAI010000227 DAOSAI010000000
VERSION     DAOSAI010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14145_length_4016_cov_3.424135"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="PLM83_07810"
     CDS             complement(<1..1415)
                     /locus_tag="PLM83_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOK71344.1"
                     /translation="MRFEELTVKSQEAIARAQSIAQANQHQSVAPMHILAALLEQEEG
                     VVSLILSKLGVSAEVLTRTVASSLEKLPKVYGQEAGQYLSTATASLLDDASSEAGRLK
                     DEYVSTEHILLALAGADDDSGRLLRSMGVTKDAIYKALVDVRGTHRVTDQNPEDKYQA
                     LERYTRDLTDLARKGTLDPVIGRDEEVRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIAAGDVPDSLKNKRVLSLDLGALVAGSKYRGEFEDRLKAVLKDIEDAEGQIILF
                     IDELHTLVGAGAAEGSIDASNMLKPALARGELRAVGATTIDEYRKYIEKDAALERRFQ
                     PVMVDEPSVEDTIAILRGLKERYEVHHGVKIKDSALVAAAVLSDRYISDRFLPDKAID
                     LMDEAASKLRMEIDSMPAEIDEVERRIVQLEIERHALDKEDDESSRERLIRLNEELKE
                     LKEKSEAMKAHWQKEKETIAR"
     gene            complement(1412..2392)
                     /locus_tag="PLM83_07815"
     CDS             complement(1412..2392)
                     /locus_tag="PLM83_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOK71345.1"
                     /translation="MEFKDYYKVLGVSKDATEEEIKSAYRRLARQYHPDVNPGDSSAE
                     ERFKAIAEAYEVLGDPEKRKRYDMLGADWDHYSKTSAGWPPGGRRTQPGYGRTTFTFG
                     NLSDADFSDFFKQFFGDLGLGDHLFGHDLHDLDERSFSRRRPVKRDIEHELEIGLDEA
                     FTGSQRSIEIVDPTDGSRRKIVVDIPPGVRDGTRLRIAGEGAGAPGGRGNLYLKVRIR
                     PHRGYQLRGDDLYVKVNVPLTVAVLGGEVQLQTVTGDTLSVRVPPETQNGQVLRLRGL
                     GMPSTKGGKRGDLMVKVNVVLPSNLTEQERELFRELERLTSASSSGRDEA"
     gene            complement(2436..3032)
                     /locus_tag="PLM83_07820"
     CDS             complement(2436..3032)
                     /locus_tag="PLM83_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOK71346.1"
                     /translation="MSPDTQDMRLGNIQSSDSSEVTGAEANGEHTPEDLPSNADMVNE
                     GDELRLKLAEAEAKAEENWNMYLRALADLENYQRRTARDLEFTIRSGKKEMFLKLLAI
                     VDDMERALAADADYESLHQGVEMIMRKLLDALASEGVKPIDALNEPFDPRLHEAVAAC
                     DGEDVETETVVEEFRRGYTYCNECLRPTMARVAVPKSD"
     gene            complement(3168..3614)
                     /locus_tag="PLM83_07825"
     CDS             complement(3168..3614)
                     /locus_tag="PLM83_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOK71347.1"
                     /translation="MALMNYDPWQEFTEMRRVFNRMFGDGFLGLPMEEQITHGSLLMP
                     VDIAETPEEFVIMAELPGMDPDDIEITLTDNQLTIKGQRAAEYEDKRENYLRTERRFG
                     SFCRSFALSSPVAEDKVSANYKNGILEVHLPKSEGAKPKQIEIRNE"
     gene            complement(3753..>4016)
                     /locus_tag="PLM83_07830"
     CDS             complement(3753..>4016)
                     /locus_tag="PLM83_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71348.1"
                     /translation="ELEELKKAIADDDMESVKRFMDQVREASYKMSEEIYKAAQEASG
                     GQGEGDYGSDSGDATGAGGFDSANSDDDDVIDADFRTVDGDDN"
BASE COUNT          766 a         1160 c         1009 g         1081 t
ORIGIN      
        1 atcctggcaa tagtctcttt ttccttttgc cagtgtgctt tcatggcctc agatttctct
       61 ttgagctcct tgagctcttc gttgagcctg atgagccttt ctcgggatga ttcatcatct
      121 tctttgtcga gcgcgtggcg ctcgatctcc agctgcacaa tacggcgctc cacttcatcg
      181 atttcagcgg gcatcgagtc gatttccatt ctcaactttg acgcagcttc gtccatgagg
      241 tctatggcct tatcaggcaa aaaccggtcg gatatatacc gatccgacaa aacggccgct
      301 gccacgagag ccgaatcctt tatcttaact ccgtgatgga cttcgtatct ttccttcagt
      361 cccctcagta tggcgatggt gtcctcaaca gagggctcat ctaccatcac aggttggaac
      421 ctgcgttcga gcgcggcatc tttctcgata tacttacgat actcatcgat agttgttgcg
      481 ccaactgctc tcagttcgcc tcgggctagt gcgggcttaa gcatgttcga ggcatcgatt
      541 gagccctcgg cagctccagc acctacgagt gtgtggagtt catcaataaa cagaattatc
      601 tgtccttcgg catcctctat gtctttcagc actgctttga gccggtcttc gaattcgcct
      661 ctatacttgc ttccggcaac tagtgcacct agatctagcg agagcacccg cttgttcttg
      721 agactgtcag gcacatctcc ggcggctatg cgttgcgcga gaccttccac gatggcagtc
      781 ttgccaacgc ccggctctcc gatgagaacg ggattattct ttgttcgcct agagaggacc
      841 tggatcaccc ggcggacttc ttcgtcccga cctatgacgg gatctagagt tcctttgcgc
      901 gccaggtcag taaggtctcg ggtgtatctt tccagtgctt gatacttgtc ttcggggttc
      961 tggtcggtca cccgatgcgt gcctcgcaca tcgacgagtg ccttgtatat ggcgtccttg
     1021 gtcactccca tcgatctgag caggcgccct gaatcatcat cagctccggc caaggccagg
     1081 aggatatgct cggtacttac gtactcatcc ttaagccgcc cggcttcaga tgaggcatca
     1141 tcgagcagac tggccgtggc tgtagacaag tactgaccgg cttcttggcc atagaccttt
     1201 ggaagcttct ctaaggatga agcaacggtc cttgtcaata cctcggcaga aacgccaagc
     1261 tttgatagga taagagagac tacgccttcc tcctgttcaa gaagggccgc tagaatgtgc
     1321 atcggcgcaa cgctttgatg ctggtttgcc tgagcgatgc tctgcgcgcg cgcgattgcc
     1381 tcctgagatt ttactgtaag ttcttcaaat ctcatgcctc atccctccct gacgagctgg
     1441 cgctcgttag tcgttcaagt tctcggaaga gttcgcgctc ttgttctgtt aggttactgg
     1501 gcaagacaac atttaccttg accatcaaat cgccacgttt tcctcccttg gttgacggca
     1561 tgccaagtcc gcgcaatcgc aacacttgtc cgttctgggt ctccgggggc actcttaccg
     1621 aaagtgtgtc ccctgtcaca gtctgcagtt ggacttcgcc tccgagaacc gcgacagtga
     1681 ggggaacgtt caccttcaca taaagatcgt cgccacggag ctggtacccg cgatgggggc
     1741 gtatccgcac cttaagatag agattgccac ggcctcccgg agctcctgct ccttcgccgg
     1801 caattcgaag gcgtgtgcca tcgcgcaccc ccggaggaat atcgactact attttccgcc
     1861 gagacccgtc ggttgggtcg acgatttcga tcgaacgttg gctgccggtg aatgcttcat
     1921 caagaccgat ttcgagttca tgctcgatat cacgcttgac cggcctgcgt cggctgaaac
     1981 tgcgttcgtc gagatcgtgc agatcatgtc cgaataggtg gtctccaagt cccaaatctc
     2041 caaagaattg cttaaagaaa tcgctaaaat cagcgtccga aagattgcca aaggtgaacg
     2101 ttgtgcgccc gtacccaggt tgggttcgtc gtccccctgg aggccagcca gcgctggttt
     2161 ttgagtaatg atcccagtcg gcgccgagca tatcgtagcg ctttctcttt tcaggatcgc
     2221 ccaacacctc ataggcttcg gctatcgcct tgaagcgttc ctctgccgaa ctgtcaccag
     2281 gattgacgtc agggtggtac tggcgcgcaa gtcgcctata ggcggacttt atttcctctt
     2341 cggtcgcgtc cttggatacg ccaaggactt tatagtaatc tttgaattcc atagtccatc
     2401 tccccggcgc gcccatgcca ttggccgtgc gcagcctagt ccgatttcgg tactgctacg
     2461 cgggccatgg tcggcctcaa acattcgttg cagtatgtgt acccgcgcct gaactcttcg
     2521 actacagtct cggtttcgac atcctcaccg tcgcatgcgg ccacggcttc atggaggcgg
     2581 ggatcgaatg gttcgttcag agcgtctata ggtttgaccc cttcagatgc cagtgcgtcg
     2641 aggagttttc tcataatcat ctcaacgcct tgatgaaggg actcgtagtc agcgtctgcc
     2701 gctagtgccc gctccatgtc atcgacgatt gccaggagct tgaggaacat ctccttcttg
     2761 ccgctcctga tggtgaattc taggtcccgc gccgtcctgc gttggtagtt ttcgagatct
     2821 gccaaggcgc gaagatacat attccagttt tcctcggcct ttgcttcggc ttcagccaat
     2881 tttagtctca actcatcacc ctcattgacc atgtccgcat tggacggaag atcttcgggt
     2941 gtgtgctctc cgttagcttc agcaccagtg acctcgctgc tgtcggacga ttggatattg
     3001 ccgaggcgca tgtcttgcgt gtctgggctc attatctaca ccaccgctcg ttattggtaa
     3061 gtggctgttt ggtgaggctt gcgagacggt ccgtaagcag tgaccgccgt tttccggtct
     3121 gtcgtcgccg cttacggacc gcacgccacg cctacagttc agccctgtta ctcattacga
     3181 atctcgatct gttttggctt ggcgccttcc gatttcggca gatgcacctc gaggatcccg
     3241 ttcttgtaat tagctgacac tttgtcttca gcaacaggtg aagacagtgc gaagctcctg
     3301 cagaagcttc cgaagcgtct ctctgtgcgc aggtagttct cgcgtttgtc ttcgtattcg
     3361 gcagcacgct gccccttgat cgtcagctga ttgtctgtaa gggtgatttc gatatcgtca
     3421 ggatccatcc caggcagctc tgccatgatc acgaactctt ctggggtttc agctatgtcc
     3481 acaggcatta acaggctgcc gtgtgtgatc tgctcctcca tgggaagccc aaggaatcca
     3541 tctccaaaca tgcggttgaa aacacgtctc atttccgtaa actcctgcca cggatcgtaa
     3601 ttcatcaacg ccatcaaacg cacctccagt ttaacaatgt agttttgtct cgaaactatg
     3661 tctcggtctg ccctcatagg gtagggcagg gcttcgactc attcggaagg cccgcgcagg
     3721 ggcctagtcc gagttcaaga tggctcactc gctcagttgt catcgccgtc tacagttctg
     3781 aagtctgcat cgatcacgtc gtcatcatcg ctatttgccg aatcgaaccc tccggcgcct
     3841 gtggcgtccc cggaatcaga gccgtagtca ccttcaccct ggccgcctga ggcttcttgc
     3901 gctgccttgt atatttcctc tgacatcttg taagaggctt ctctgacctg gtccatgaag
     3961 cgctttacag actccatgtc gtcgtcggcg atggcttttt tcagttcctc aagttc
//
LOCUS       DAOSAI010000228         4013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14153_length_4013_cov_3.698080, whole genome shotgun sequence.
ACCESSION   DAOSAI010000228 DAOSAI010000000
VERSION     DAOSAI010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14153_length_4013_cov_3.698080"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="PLM83_07835"
     CDS             <1..1546
                     /locus_tag="PLM83_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK71349.1"
                     /translation="SLQLLTQVEPATNILTVKGVHRIPGVEIDCEYGMLANGSSSGAQ
                     ALRASGRSKLGPVEAVVTVQNADRGYTAIDNPGIDMSLKLKSLSKLPVSGTLLLQKHD
                     DGELHSRKLEANAVWKLDSTELGLKYSDTAKWDDGSDDLGRSRQASVSLNVFDSSNAS
                     LKEEISVQNCSGMLGNPGSWTVAYALKKQLIESRWKWTSCLSASMPLSQGASVHVGLG
                     ADASYQVVPGLEMRGGFRAGTIRQSGLTVSAGATYKPSDWAALSIDASCKIVDGGVRD
                     LGVKVGQTAFFDIPLARRSDVGEISGKVVGVDGKGMPGIIVNAGGFSTVTDSSGNFRF
                     PAVVEGEQYVSVSVRELGPEYLVMPSSAVSVKVERNTESTVQLTVARSGRIEGIVRFS
                     SDEGSRAVPDEMIWRIADGISTQSAPKLLSGLLVEVSGARRYVVSLDFEGRFAIDHLP
                     PGDYRVTIDRSSVPSLYELTPSAMNITLGEGEKASAEFAIYQVLREYDFTEGGEIGNE
                     LIAEEQ"
     gene            1899..3536
                     /gene="pgi"
                     /locus_tag="PLM83_07840"
     CDS             1899..3536
                     /gene="pgi"
                     /locus_tag="PLM83_07840"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOK71350.1"
                     /translation="MSVTELPAWRALAEHRLEMENIHMRELFASDPERFDRFSIRLWD
                     LLFDYSKNRITDETMSLLFNLAEQARLAEAIEAMFNGERINTTENRPALHVALRNRSN
                     RPILVDGTDVMPEVNAVLSQMRRFSEAVRSGDWKGYTGKAITDVVNIGIGGSDLGPRM
                     VVHALKPYGHDRLHVHFVSNVDPTDISETLSDLAPETTLFLIASKTFTTQETMMNANT
                     ARNWLLRHAGEDAAVAKHFVAISTNRDAVEAFGIDHRNMFVFWDWVGGRYSLWSAIGL
                     SIALYVGMDDFESLLAGAHKVDEHFRTAPLDRNIPAIMGLLGIWYNNFWGAETCAVLP
                     YDQYLAYFPDYLQQADMESNGKSVTRTGGPVDYQTGPVLWGQPGTNGQHAFYQLIHQG
                     TKLVPCDFLAAAQSHNPIGDHHEVLVANFLAQTEALMKGRTSDETRAELAAQGCTGQE
                     LELLTAAKTFEGNKPTNTFLYKKLTPETLGMLIALYEHKIFTQGVIWNVNSYDQMGVE
                     LGKELASKILPELKDVKPAVGHDSSTVGLINAFKSMR"
     gene            3683..4000
                     /locus_tag="PLM83_07845"
     CDS             3683..4000
                     /locus_tag="PLM83_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71351.1"
                     /translation="MKRKAEKNGGVPVKFMSNIMAFIKKSESNVIAIRPNEYLFLCDE
                     NGHDMAALIAYSPEMKMDMLSEFKQDASAQYASGYSAVTGAIVDKLGAVGPVMMSGRE
                     PAN"
BASE COUNT          945 a          976 c         1187 g          905 t
ORIGIN      
        1 ttcccttcag cttttgacac aagtagagcc cgcgacgaac attctcacag taaaaggtgt
       61 gcacaggata cctggcgtcg aaatcgattg tgagtatgga atgctggcta atgggtcgag
      121 ttctggcgca caagcgctgc gtgcaagcgg caggtcaaaa cttggcccgg ttgaggcagt
      181 tgtgaccgtt cagaatgcgg atcgtggcta caccgcaata gacaatcccg ggattgacat
      241 gtcgttgaaa ctcaaaagtc tctcaaagct gcccgtatca ggcacccttc tgctgcaaaa
      301 gcatgatgat ggggaattgc atagccgcaa attggaagct aatgccgtat ggaagttgga
      361 ttcgactgaa ctaggattga aatactccga tacggcgaaa tgggacgacg gttcggatga
      421 tctcggcagg tctagacagg cctcagtctc tctcaacgtc ttcgatagca gcaatgcctc
      481 tctcaaggag gagatttctg tgcagaactg ctccggcatg ttgggaaacc ccggaagttg
      541 gaccgtggca tatgctctca agaagcagtt gattgaaagc aggtggaagt ggacctcctg
      601 cctaagtgcg agtatgccgt tgagccaagg cgcttcggta catgttggct tgggtgcaga
      661 cgcttcatat caagttgtgc ccgggctgga gatgaggggc ggttttcgcg caggcactat
      721 aaggcagagc ggcttgactg tatctgcagg agcgacgtac aagccatctg attgggcggc
      781 cctatcgatc gatgcatcct gcaagatagt ggacggcggg gtgcgtgact tgggagtgaa
      841 ggttggccag acagccttct tcgatattcc cctggcccgc cgctcagacg tgggggagat
      901 atcgggaaag gttgtgggcg tggacggtaa gggtatgccg ggtattatag tgaacgcggg
      961 agggttttca accgtaacgg attcatcggg taatttcaga ttccctgcgg tggtcgaagg
     1021 cgagcagtat gtatcggtta gcgtgcgtga actgggcccc gagtatctag tgatgccctc
     1081 gtccgccgtc agtgtgaagg tggaacggaa caccgaatcc acagtgcaat tgactgttgc
     1141 caggtcggga aggatcgaag gtattgtcag attctcttct gacgagggaa gccgtgcagt
     1201 gcctgatgaa atgatatggc gtattgcaga tggtatatcg actcaaagtg cgcccaagct
     1261 gctgtcaggg ctgctggtgg aagtatccgg agctaggcgg tacgtggttt cgcttgattt
     1321 cgagggccgg tttgctatcg accatctccc cccaggggac tacagggtaa ctatagatcg
     1381 gagctctgtg ccttcccttt acgagctgac tccgtcagcg atgaacataa cattgggcga
     1441 aggtgaaaag gcatcggctg aatttgctat ctaccaggta cttcgggaat atgatttcac
     1501 tgaaggtggc gagattggaa acgagctaat agcagaggaa cagtagcgaa agtagctggt
     1561 gcaggcaaat tgcgcgggag ctgaggtgat cagatgacgc agcgacagcc gctaatcgcg
     1621 ataaacttcg ttctgcatgt ggcgcgtttg caaagaaggc cttctatcaa ggcgggtgcg
     1681 ccatatccta tcctccatat gagcaacgcc gacaaggcgt tagcctctcc agatcacggt
     1741 gttcagctcg agggttgtac cgggttttgc ctaagaaata gccttgttcc catgctacac
     1801 ccggggtatg ataagaattg gcatgaaatg atgcagttcg cttgaatggc tccctcgttt
     1861 ttgaagagcc gagctgtctt atggggagga acaccaccat gtctgtaaca gaacttccgg
     1921 cgtggcgggc cttggctgag caccggctgg agatggaaaa catccatatg cgcgagctgt
     1981 tcgcatctga cccggagaga ttcgaccgat tttcgatcag gctttgggat ttgctcttcg
     2041 actactccaa gaaccgcatt acagacgaga ccatgtcttt gctattcaac cttgccgaac
     2101 aggcccgcct tgcagaggca atagaggcga tgttcaacgg cgagcggata aacacgacag
     2161 agaatcgccc agcgcttcac gtggccttgc ggaatcgatc caatcggccc atcctggtgg
     2221 acggcacgga cgttatgcct gaggtaaatg cggtgctttc tcagatgcgc cgcttttctg
     2281 aggcggtgcg cagcggcgac tggaagggct acaccggaaa ggccatcacc gatgttgtca
     2341 atatcgggat tggaggatca gatctgggtc ctcgcatggt tgtgcacgcg ctaaagccct
     2401 acggccatga ccgcttgcac gtccactttg tgtcgaatgt agatcccacg gacatttcag
     2461 aaaccctatc cgaccttgcc ccagagacga cgctcttcct catcgcttcg aagactttca
     2521 ctacgcaaga gaccatgatg aacgccaata cggctcggaa ttggcttttg cgccatgccg
     2581 gagaagatgc agctgtagcc aagcacttcg tggcgatctc caccaatcgc gacgcggttg
     2641 aagcgttcgg gatcgaccat cgcaacatgt tcgtcttctg ggattgggtt ggcggccgtt
     2701 actcgctctg gtcggccatt ggactttcga tagctctcta tgtgggcatg gacgatttcg
     2761 aatctctgct cgcaggcgcg cacaaagtag acgagcactt tcgcactgcg cccttggacc
     2821 ggaacatccc ggctataatg gggttgctcg gcatatggta caacaacttt tggggtgccg
     2881 agacctgcgc tgttctccct tacgatcagt acttggctta tttccccgat tacctgcagc
     2941 aagcggacat ggaaagcaac ggcaagagcg tgaccaggac gggggggccg gtggactatc
     3001 agactgggcc agtactttgg ggacaaccag gaaccaacgg gcaacacgct ttctatcagc
     3061 tcattcacca gggcacgaag ctggtcccgt gcgatttcct ggctgcagca cagagccata
     3121 atcccattgg cgaccatcac gaagtgcttg tagccaactt cctggcgcag acagaggcgc
     3181 tcatgaaggg ccgaacttcc gacgagaccc gggcggagct tgcggctcaa ggctgcactg
     3241 gtcaagagct ggagttgctg accgctgcga agacctttga aggaaacaag cctacgaaca
     3301 cgttccttta caagaagctt actcctgaaa cccttggcat gttgatcgct ctgtacgagc
     3361 acaagatatt cactcagggc gttatttgga atgtgaacag ttatgaccag atgggggtcg
     3421 agctagggaa ggaactcgcc agcaagattt tgcccgagct gaaggacgtc aagcccgcag
     3481 tcggtcatga cagcagcact gtcgggctaa tcaatgcgtt caaaagcatg agatagccga
     3541 tgatgtcatc cgtttggcag ccggagagtt cgtagttcgg cggtcaaaat gtgcatagct
     3601 aactatgcct ggggccagtg gttgctctcc attcttagct atatatgcat gcaagtctgt
     3661 gcgagttggc ggcgaaacat ccttgaaaag aaaagccgag aagaacggag gcgtgccggt
     3721 gaagttcatg tcgaacatta tggccttcat aaagaagtcg gaatcgaatg tgatagcaat
     3781 caggcccaat gagtacctgt ttctttgtga tgagaatgga cacgatatgg ctgctctgat
     3841 cgcgtattcg cctgaaatga agatggacat gctaagcgag ttcaaacaag acgcaagtgc
     3901 ccagtatgcg tccggttaca gcgcagtgac tggagcgata gtcgacaagc ttggcgcggt
     3961 gggaccggtc atgatgagcg ggcgagagcc ggcgaattga tgcagattgt tgc
//
LOCUS       DAOSAI010000229         4006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14182_length_4006_cov_3.791445, whole genome shotgun sequence.
ACCESSION   DAOSAI010000229 DAOSAI010000000
VERSION     DAOSAI010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14182_length_4006_cov_3.791445"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLM83_07850"
     CDS             complement(<1..278)
                     /locus_tag="PLM83_07850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOK71352.1"
                     /translation="MYKLIACDIDGTLLRSDGSTSPRTIKAIRLAQACGAKVSIATGR
                     RIISAMPVIDAFRMVHPAVVHCGAVVLQPGSESILMAQQIPRDTALHV"
     gene            1018..1665
                     /locus_tag="PLM83_07855"
     CDS             1018..1665
                     /locus_tag="PLM83_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71353.1"
                     /translation="MSDDRKTRLAKAEGRMRLLPGVTRVRILELADGDEIHVMVEGCY
                     DQDEIKRLKKDIETVYLLETGESIDYRRISIAQIREKHPDDHAGSNGRPVLSRISVEY
                     GQGGAVVAYVDIEFGGECYSGSANGRTIEEPLSNIVKRATLKSIEKLLGEKYALQADC
                     EITDGRVISEITLIDSSSGQRQRYLGAAYKRDDISTAVARSILQALNRQFELLVD"
     gene            1973..3226
                     /locus_tag="PLM83_07860"
     CDS             1973..3226
                     /locus_tag="PLM83_07860"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOK71354.1"
                     /translation="MPRDRHVLHDVYVWSLVALSVVLVGRFIEWDMPLGSALAFIGLL
                     WASSLSVINLRNTVVLMLLPIMMTPVVKYNPGATAVIAAIGSVSIQEIRILRENNSPY
                     LFILKVLGNRASIMLCALLSGLVFHSLWPAATVDFGDVRFLAAFAIAGLSWVMLSGVI
                     INFQILLSEPKFEVKIDPAVIIGSSRSLVPSVIMGLMAMAMYEYGGLILFTLAQLFFI
                     SNKNYTQRALVDKENAEQINLALARIIDSKDHYTGGHSERVAILARQLAEACRLPGAD
                     VDRIEYIARLHDVGKVNLPDEILSKPGRLTAEEYDAVKRHPEWGAELIRGMERIYSDR
                     DYRAILEHHERYDGAGYPYGKKGEEISLWGRILAICDAYDAMTSERVYREALPVEAAR
                     FELKRNSGTQFDPELVDLFLSNVQE"
     gene            3231..>4006
                     /locus_tag="PLM83_07865"
     CDS             3231..>4006
                     /locus_tag="PLM83_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOK71355.1"
                     /translation="MERSMIFGIIGGLGLFLYGMNLAGDGLRRAAGDRMRNILEVLTS
                     TPLKGVLVGMLVTAVMQSSSATTVMLVSFVNAGLMTLRQALGVIMGANIGTTITAQLI
                     AFNLADYALPAIGIGFLIHMVARRKVWKSVGQIVLGFGILFLGLSLMSTSVIPLQDSP
                     AFGKAMKTFGTNPVLGVLIGLVTTLVVQSSAATIGMLMALAISSPDLVTLEVAIPILL
                     GDNIGTCITAILSGIGASVTARRTAMAHLMFNVFGALIFLP"
BASE COUNT          933 a          899 c         1170 g         1004 t
ORIGIN      
        1 cacacgtgca gcgctgtatc gcgtggaatc tgctgtgcca ttagaatact ctcggagccg
       61 ggttgcaaca caacagctcc gcagtggacc acggcaggat gcaccattct gaacgcgtcg
      121 attactggca tggcggatat tatccttctg ccagtggcta tcgatacctt tgccccgcac
      181 gcctgggcaa ggcggatggc cttgatcgtt cttggactgg tgctgccatc acttcgaagg
      241 agcgttccgt ctatgtcgca agctatgagt ttgtacactg ggcgctcatc ctccgttgag
      301 ttcattctac ttgaagactg ttcacctaag attattgcag tgccgattac gccgaggaca
      361 ggaacgaata gtcgtcggag cgcaaacagg caaagcactt atgccaagaa tgaacaggca
      421 atgccgagat aggccgaggt tgagagctat ctgtggccta gctacgacgg atttgggcgg
      481 cgggccgtcg ggaacgacag aacatgccat gactatcgtc gctaggcctg cctggcaagt
      541 acttgttgcc aatgtgccgt aggggtccgt tgaggactgt atacatcgtc catgccaaag
      601 aagaggtgag tgggattgga acgttgcgaa gacaatgggc gtctcatacg ccctggccta
      661 cacttcctgc gacgacaacc cagccccttg ctgcggatag aagtatacct gccaagcgag
      721 aaaatggcaa gaggccgtta tcgtccagcc caacgctgcg ggaatgtaaa gcccggcagt
      781 gttctcacga tacaaggcgg tcggtatttc ggtaaagggg cgattcggtc taatagggct
      841 ttggtgttca cagaggcagc tgaaatgcta gtctttaccg cccggggcga cacctcaatt
      901 atatttcatg gttgtttcgg aattgaaggg atttggcttg cggagtcgaa ttcaaatccg
      961 aagttatcgg agtgttgcaa cggcggcaat ggtccgatgt tcttgggggg tattcgggtg
     1021 tctgatgaca gaaagacgcg attggctaaa gcagaagggc gcatgcgcct attgcctgga
     1081 gtcacccgag tgcggattct tgagttggcg gatggagatg agatccatgt aatggttgag
     1141 ggctgttatg accaggatga gatcaaaagg ctcaagaagg atattgagac cgtctacttg
     1201 ctggaaactg gcgagagcat tgattatcgt agaatcagca tagcacagat cagggagaaa
     1261 catccggatg accacgccgg ttccaacggc aggcccgttc tcagcaggat ctccgtcgaa
     1321 tatgggcagg gaggcgcagt agttgcctat gtggacatag agtttggcgg agagtgttac
     1381 tcaggttcgg cgaatgggag aacgattgaa gaaccgctga gtaatatagt caagcgcgcc
     1441 acgttgaagt ccattgaaaa gttgttgggc gaaaaatacg cgttgcaggc cgattgtgag
     1501 ataacagatg gaagggtgat ttccgagatt actctcatag atagcagctc cgggcaacgc
     1561 caaaggtatt tgggggctgc ctacaagagg gatgacattt ccacggcggt tgccagatct
     1621 attcttcagg ctttaaaccg ccaatttgag ctcctagtag actgagcaga agaattaccc
     1681 gtcgcggcgc gagatgagag tgagagatga gctgagcctc ccaggttaat tacgaccgat
     1741 gggtggtgaa gcgagtgaag aaatttgctc tgtcggcatt gatagctaca gccatgacgc
     1801 tgctgatgtc agtcatggct tttgctgaca catttctgat gaagtaacaa gataggcgcc
     1861 gttaaaactt cgccaagcgc cgccaatagc ggcttgccgc ggcgggcctt ttgtttgatg
     1921 gggatggtgt gattgcgatc aaagcatcgg ggacatggcg ggagtgatag gattgcctcg
     1981 ggacaggcac gttttgcatg atgtttatgt gtggtcgctt gtagctctct cagttgtgct
     2041 agtaggacgc tttattgaat gggatatgcc gctcggatca gcgctggcgt ttatcggatt
     2101 gctttgggca tcatcgctgt ctgtcataaa cctgagaaac accgtggtct tgatgctcct
     2161 tcccataatg atgacgcctg ttgtcaaata caaccctggg gccactgcag ttattgcagc
     2221 cattgggagt gtgagcatcc aggagatccg gatcttgagg gaaaacaatt cgccgtatct
     2281 tttcatcctc aaggttttgg ggaaccgggc aagcatcatg ctgtgtgccc tattgtccgg
     2341 attggtattc cattcgcttt ggcccgcagc cactgtggat tttggagatg tcagattcct
     2401 ggcagcgttt gcaattgctg gtctatcatg ggtgatgttg tcaggagtca taatcaactt
     2461 ccagatcctg ctctcggagc ccaagttcga ggtgaagatc gatcctgccg tgatcatcgg
     2521 ctcatcgcgc tcgctggtgc cgtctgtgat catgggattg atggcaatgg ccatgtacga
     2581 gtatgggggc cttattctat ttaccttagc tcaactcttt tttatctcga acaagaacta
     2641 cacacagcga gcccttgtag ataaggaaaa tgctgaacag atcaacttgg ccctggcaag
     2701 aatcatcgat tccaaagatc actataccgg aggccattct gaacgtgtgg cgatattagc
     2761 ccgtcagctt gcagaggcat gtagacttcc cggagcagat gtggacagaa tagagtatat
     2821 cgccaggctt cacgatgtgg gcaaggttaa cctccccgat gaaatactct cgaaacccgg
     2881 ccggcttact gccgaggaat atgacgcggt aaagaggcac ccagaatggg gggctgagct
     2941 gattcgaggc atggaaagga tatacagtga tcgcgattac agagctattc ttgagcatca
     3001 cgagagatat gatggcgccg gatatcccta tggcaagaaa ggcgaggaga tatcgctttg
     3061 ggggcgcatc ctggcgatat gtgatgctta tgatgcaatg acgagcgagc gcgtttatag
     3121 agaggcgctt cctgtcgaag cagccaggtt cgagcttaag cggaattcag gaactcagtt
     3181 cgatcctgag ttggtagatc tgtttctgtc aaacgttcag gagtgatgaa atggaacgca
     3241 gcatgatatt tggcattata ggcggtttgg gcttgttcct gtacggtatg aatctggctg
     3301 gagatggcct caggcgcgct gctggtgaca gaatgcgcaa catattggag gttttaacct
     3361 ctactccctt gaagggagtc ctggttggta tgcttgtgac ggccgtcatg caaagcagca
     3421 gcgcgactac tgtcatgctt gtgagcttcg tgaatgcagg gttgatgacg ttgcggcagg
     3481 cgcttggagt cattatgggt gcgaatatag gaacgactat cacagcacag ctaatcgcgt
     3541 ttaacttggc tgattacgct ttacccgcaa tcggcatcgg tttcttgatc catatggtgg
     3601 ctcgtcgcaa ggtctggaaa agtgtaggcc aaatagtgtt gggctttggg atcttgtttc
     3661 tcgggctttc actgatgtcg acgtctgtta taccactcca agatagtccg gcttttggta
     3721 aggcgatgaa aacctttgga acgaatccgg tcttaggtgt cctgataggg cttgtgacta
     3781 ctttagtggt gcaaagtagt gcggctacca taggtatgct gatggcgttg gccatctcat
     3841 cgccagacct tgttactctt gaggtggcaa tccccattct tttgggcgac aatataggca
     3901 cctgcataac tgcgattctt tcggggatag gcgccagtgt gactgccagg cgcaccgcca
     3961 tggctcactt gatgttcaat gtcttcggag cgctcatatt cctgcc
//
LOCUS       DAOSAI010000230         3985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14281_length_3985_cov_3.594656, whole genome shotgun sequence.
ACCESSION   DAOSAI010000230 DAOSAI010000000
VERSION     DAOSAI010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14281_length_3985_cov_3.594656"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1207)
                     /locus_tag="PLM83_07870"
     CDS             complement(143..1207)
                     /locus_tag="PLM83_07870"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK71356.1"
                     /translation="MQQRFIRRVPARKRQGSLTVIAIPSSGEPPRSVGIPTQMLAICA
                     AALIGVIGIFVISYLNMSRGIATLNEAKMDSDNAIEQLLSENQTLKLEKQERLAKMQE
                     LASKTQMLEATVSRIADEKAEIWAIVKGSDAAQKNASSSNAVSSRGGASRGGAPGAER
                     ESPEAVLDAVANAPAVDVSADAFDGEQYLVETMAEELEMLLVATSAMQKDLSDLRVSA
                     ETYRSKLDHTPSGWPVNGRITSAYGNRRHPILRITRFHEGVDIAATHGTPVVAAADGV
                     VVLSGWSGGYGNAVEISHGFNCSTFYAHNSRNVVKVGQKVKKGQIIAYVGSTGLSTGP
                     HCHYEVRIGGRHVDPAPYMK"
     gene            complement(1374..1940)
                     /locus_tag="PLM83_07875"
     CDS             complement(1374..1940)
                     /locus_tag="PLM83_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HOK71357.1"
                     /translation="MDWRRDRGLVWPGLDMEQSAVIGLICAQQQLVPMILSIILLISV
                     VVFINATNRIRRLEKAYRSLLGTESEEKDIEQIIMEYIRKTDMLQDQLDETRKELERA
                     QDAALAYIQKVGIVRYDAFDDTGGEQSFSMALLDANANGVVVSGLSGREETRVYAKPV
                     KGGRSPHTLSDEEIQAIARAIESSKEKL"
     gene            complement(1967..2833)
                     /locus_tag="PLM83_07880"
     CDS             complement(1967..2833)
                     /locus_tag="PLM83_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOK71358.1"
                     /translation="MATKRGLGKGLNAIIGEAMLNEESHELRGIEIPLDQLTANPFQP
                     RAEFDPDKLEELAESIRAHGVLQPIVVRRAGENYQIIAGERRWRAAQMAGLQTIPAVV
                     KELDDPGMIQVALIENLQREDLNPIEEASAYRKLMDEFSMTQEQLSGTLGKSRSTIAN
                     AVRLLNLPDEIQEHVSRGRLTGGHARCLLALDDAKLQLEVAKEVIDKGLTVRQTEELV
                     RRLVKNVSRETIEQAPQVPDPDVVAIIRHLSERLGTKVKITGSSGKGKVEIEYYSEDD
                     LGRILDIILGIA"
     gene            complement(2836..3603)
                     /locus_tag="PLM83_07885"
     CDS             complement(2836..3603)
                     /locus_tag="PLM83_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK71359.1"
                     /translation="MGRVIAVANQKGGTAKTTTAVNLGAYLAMAGRRILLVDMDPQGN
                     ASSGLGVDKRAVESCIYNVLINDIEIEKAILNTGVQGLDIVPATLNLAGAEVEMVSLM
                     SREHRLRKALEPVRVIYDYCLVDCPPSLGLLTVNALTASDSVLVPIQCEYYALEGLGQ
                     LMRTIDLVKRHLNPELEIEGIVMTMYDARTNLSQQVVEDVKNNSGQHVFEAVIPRNVR
                     LSEAPSYGVPISMYDDRSKGAEAYRSLANEIMQREGA"
     gene            complement(3700..>3985)
                     /locus_tag="PLM83_07890"
     CDS             complement(3700..>3985)
                     /locus_tag="PLM83_07890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK71360.1"
                     /translation="RGVANMGVLAEYCLPFVRPGGLFVAMKGPRVVDEMDAGGKAIEI
                     LGGSQAILRHLALPYDAGERVLVAVRKERNTPAKYPRKAGAPARKPLGDA"
BASE COUNT          838 a         1246 c          889 g         1012 t
ORIGIN      
        1 cgcaccgacc cgctcgcctc gatggagccc tctaccgtgg tacccttgcc tatgtaagta
       61 tcaattcttg ccgcgccagg cgccttctcc ggccttctac gaaacatgcg atcccctcct
      121 gctaagtctg tgctaaagcg ctctatttca tgtatggcgc gggatccacg tgacgccctc
      181 ctattcgcac ctcataatga cagtgcggcc cagtgctcaa gccagtgctg cccacatagg
      241 cgattatctg ccctttcttc accttctgcc ctaccttcac cacattcctc gagttgtggg
      301 cataaaaggt gctgcaattg aagccatgtg aaatctctac agcattgcca tacccgccgg
      361 accagccaga caagactact actccatcgg cggcagccac tactggcgtg ccatgcgtcg
      421 ccgcaatgtc aacgccctca tgaaagcgag tgatccgcag tatcggatgc cgcctgttgc
      481 cgtacgctga tgtgattcgc ccgttgacag gccatccaga cggcgtgtgg tccaacttgg
      541 agcgataggt ctcagcggag acgcgcaagt ccgacagatc tttttgcatc gccgacgttg
      601 caaccaacaa catttcaagt tcttctgcca tcgtctccac aagatactgc tctccatcga
      661 aagcgtcggc gctaacatct acggccgggg catttgccac ggcatcgagc accgcctcag
      721 gggattccct ttctgccccg ggagcgccac ctctcgaagc tccaccccta gatgaaaccg
      781 cattgcttga tgaagcgttc ttttgggccg catccgaacc tttcactatc gcccatatct
      841 ccgccttctc gtcagcaatc cggctcactg tcgcttccag catctgtgtc ttgctcgcaa
      901 gttcctgcat tttggcaagt cgctcttgct tctcaagctt gagcgtctga ttctctgaaa
      961 gcaattgctc aatggcattg tcgctgtcca tctttgcctc attgagcgtc gcaatccctc
     1021 ggctcatgtt caaatacgag atcacgaata tgccaattac ccctattagt gctgcagcgc
     1081 agatcgcgag catctgcgtc ggaatgccaa ccgaccttgg aggttctccc gaggatggaa
     1141 tggcgatgac tgtaagggac ccttggcgct tccgggcagg aactcgccga atgaaccttt
     1201 gctgcaccgg tcgcccccct ttcgaacccg ttcaagatga ctcaagaaga agtctgcccc
     1261 ctggcagaca ccaacatcac ggacatattc gccatatgaa tccgatttcc tgcaacatct
     1321 tccattaatg attcccgatt tcccgacttt cgttcagttc gccttcctgg gcactatagt
     1381 ttctctttgg atgactcaat ggctctagca atagcctgta tttcctcatc agataacgta
     1441 tgaggggaac gcccgccctt aacgggtttg gcatagacac gagtctcctc acgccccgac
     1501 agcccactga cgacgacacc gttagcgttg gcgtccagca atgccatgga aaagctttgc
     1561 tctccgcctg tatcgtcaaa agcatcatat cgaacaatgc cgaccttctg tatgtaagct
     1621 agagccgcat cttgtgcgcg ctccaattcc tttcgagtct catcaagttg gtcctgaagc
     1681 atgtccgtct tgcgaatgta ttccatgatt atctgttcta tatccttctc ttcgctctcc
     1741 gtcccgagca aagagcggta tgccttctcc agacgtctga tcctgttcgt tgcattgata
     1801 aaaacaacca cgcttatcaa tagaataatt gatagaatca ttggcacgag ctgctgttgt
     1861 gcacagatga ggccaattac tgcactttgt tccatatcaa gcccaggcca cacgaggccc
     1921 cggtccctcc tccagtccat caactttcct tccaccggcg ttgccgtcaa gcaatgccaa
     1981 gaatgatatc gagaatcctt cctagatcgt cttctgaata atactctatc tctaccttgc
     2041 ccttgcccga ggacccggtt attttcacct tcgtcccgag cctttcgctc aggtgcctta
     2101 tgatagctac cacatccggg tcgggcacct gtggagcctg ttcaattgtt tcacgtgaaa
     2161 catttttcac tagccttctc actagttcct ctgtttgacg aaccgtcagc cccttatcga
     2221 tgacttcctt tgctacctcc agctgcaact tggcgtcatc aagggctagg aggcatcggg
     2281 catgccctcc tgtcaggcgc ccacgtgaga catgctcctg tatttcgtcg gggagattaa
     2341 gcaatctcac agcgttagct attgtcgatc ggctcttgcc caaagtgccg gatagctgtt
     2401 cctgtgtcat actgaattcg tccatcagct tccgataggc tgaagcctcc tctatcgggt
     2461 ttaagtcttc cctttgcagg ttctcaatga gcgccacctg tatcattcca ggatcgtcaa
     2521 gttccttgac gaccgcaggg atagtttgca acccagccat ctgtgcggcc cgccaacgcc
     2581 tctctccggc gatgatctga tagttctcgc cagcccgcct taccactata ggctgtagaa
     2641 ccccatgtgc cctgatggat tcagccagtt cctccaactt gtccggatca aactcggctc
     2701 ttggttggaa ggggttcgcg gtcaactgat ccagcggtat ctcgataccc cgtaattcat
     2761 gcgactcctc attgagcatg gcctctccta tgattgcatt gaggcccttg cccaatcccc
     2821 gcttagtcgc catggctaag ccccctccct ttgcataatc tcgttggcca gacttctata
     2881 cgcctcggca cccttcgatc tgtcatcata catagatatc ggaactccat agctcggagc
     2941 ttcgctcagg cgcacatttc tcggtatcac ggcctcaaaa acgtgctgcc cggaattatt
     3001 cttcacatcc tcaacaacct gttgcgagag attcgtccgg gcatcgtaca tggtcattac
     3061 aataccctct atctctagct ccgggttgag gtgacgtttc accaaatcga tagtccgcat
     3121 tagctgaccc aagccttcaa gggcgtaata ctcacattgt atgggcacca ggacggaatc
     3181 cgaagcagtt aacgcattta ctgttaggag gcccaacgac ggaggacagt ccactaaaca
     3241 gtagtcgtat atgaccctca ccggctcgag tgcctttctg agcctgtgct cacgcgacat
     3301 gagactgacc atctccacct ccgcccccgc caaattgagc gtagcgggaa cgatgtcaag
     3361 cccctggact cccgtgttga gaatcgcttt ttcaatctca atatcgttga tcagaacatt
     3421 gtaaatgcat gactccacgg cccgcttgtc aacacccagc ccggaactgg cgtttccctg
     3481 tggatccatg tccaccaaaa gaattcttct gcctgccatc gcaagatagg caccgaggtt
     3541 gactgcggtc gtagtcttcg ccgtgccgcc tttctggttg gctactgcaa tgaccctgcc
     3601 caatgcgtta cgcctcctta gaatgctgtt catgacttcg ccacgccgca agaatgtcct
     3661 tcactatagt gggcgattca gtatcacccg aaagtgtgat tatgcatcac ccagaggttt
     3721 tctggccggc gctcccgcct ttcgcggata cttcgcaggc gtattgcgct cttttctcac
     3781 cgcgactaac accctttctc cagcgtcgta aggaagcgca aggtgacgga ggattgcctg
     3841 gctgccccct agtatttcga tggccttgcc cccggcatcc atttcatcga ctacccgcgg
     3901 acccttcatc gcaacaaaaa ggccccccgg cctgacgaaa gggaggcagt attccgccaa
     3961 aacacccatg ttggcgactc cccgc
//
LOCUS       DAOSAI010000231         3982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14298_length_3982_cov_3.756557, whole genome shotgun sequence.
ACCESSION   DAOSAI010000231 DAOSAI010000000
VERSION     DAOSAI010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14298_length_3982_cov_3.756557"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            550..1899
                     /gene="mgtE"
                     /locus_tag="PLM83_07895"
     CDS             550..1899
                     /gene="mgtE"
                     /locus_tag="PLM83_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOK71361.1"
                     /translation="MDETYENIIEHMRALLAGGTDEALIEFATTLHPADIVDLLEELD
                     DAERSAFFHVIPADVAADIVQELEPADQAELIRLMGAARSSEILKEMDSDDAADLIAE
                     LPEAKAETLLDLMNSHGQDVRELLQYDEHSSGGIMATEYVSIQSDWTVEQTLAELRHV
                     APEAETVYYIYVLDDDEHLVGVLSLRDLVVADLSTRIDAIMNTNVLSVPVEADQEDVA
                     QLFKKYRFLALPVVDKEQRLTGIITADDILDVFEEEATEDMQKIAGTMPLERPYFSTS
                     IKELWGSRIVWLLVLFIAESFTGGIMRQFSDAMQSVIELTFFIPLLIDCGGNAGSQAS
                     TVVIRGLAVRDIKLRDVWTVMARELVVGLGLGVVMAGVGFFRAWRVGGSSDIGIIVCL
                     AVLAIVVLSTVIGALLPIIATALKVDPAVMSAPLITTIVDAFGLLIYFSIAKSVLQL"
     gene            complement(1970..2971)
                     /locus_tag="PLM83_07900"
     CDS             complement(1970..2971)
                     /locus_tag="PLM83_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOK71362.1"
                     /translation="MCRQRRFALGVVMILMTAGMSGCLKSQRAEAGIGAMSVHASIKS
                     VPSTACPPRVIALRLTMGGFEIREELPIVNHSASSLIQSLAAGVWNIDAAAVDGEGDT
                     IYEGKAKAEVRRDTVVPLKMTLNPVPGRVRFEASPEGLPESESIDAAEFWVYHAGGTS
                     AYRSYKDIRRSGGLFTVEDTGYVPRSYDMRVVFLREDGSALYESDYIGIGVCPGKRTT
                     VSWTPGFGSLEVEIGFQPYPISPKDVAVSIEDTSATVSWTPVEESMVDHYRVYWRYST
                     QERYITSRSVVVEAPASSAAVSLSSRQRGSEVRFVVVARDAQGQESLRSDEAVAEYR"
     gene            3291..>3982
                     /locus_tag="PLM83_07905"
     CDS             3291..>3982
                     /locus_tag="PLM83_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71363.1"
                     /translation="MILSRSRKPFGNISSSSPESSTDWVQCTFWAMLLSSIILSTVIV
                     RANHLCREVKNNLLSRATTAAALIDPAILSNLEEAFSLLLRPCSNRVASPNQTRPSLT
                     AAYIVSLDSQRIRFISGASAQPGSSNTLPNPGDVIGRASPEEVALIFAGHPIVRGPYP
                     SGRSKSVTACAPVIDTRTGTVVAALALEVDAADCPKPLAKGWSVIAFALALTGILGIA
                     LPRMHAENEATE"
BASE COUNT          949 a         1155 c          992 g          886 t
ORIGIN      
        1 ccctcgactt ggcccatgta gagtcgcgcc gttttcggcc ctttcgggtt agtgccccgc
       61 taagccagga tagtgtttgc atatcttccc cgggttaatt ccatcgcacc caaaagtccc
      121 gccaagccaa tcacgtaaca ataagttcct gcgagtttat cggccttgta ccgcccacag
      181 ttcaagctca aatggaccaa gatgttctcg caagttactt aggcgaaaga agctaagcaa
      241 aacgcacgtc gtccccgcag gacctcgatt ttgtgcagag attggaggcc caagacaacg
      301 ccaagagtga caatctgctg cagcaatagt ataattacca aggcaactga cacgctgttg
      361 cactgggtca tcaggtttcc agttgatcgc caaaaggagg gagaataagt ggacggagac
      421 cccggcagta cagagcacat gaataaatcc agctctgtcc agcggtcatt catccaacat
      481 gcaatcgaat ctggggcgtc gtccacctct tccaaaggtt ggtgagcgcc ccacataggg
      541 ggtgttattg tggatgaaac ctatgagaac atcatcgaac atatgagggc tctgctggca
      601 ggtggaaccg acgaggccct cattgaattc gcgactacac tgcacccggc cgacatagtt
      661 gacctgctcg aagaacttga cgatgcggaa cgcagcgcat tcttccacgt tattcccgcg
      721 gatgtcgcag ccgacatagt tcaagagctg gagcctgcag atcaagcaga gctgatcagg
      781 ctaatgggcg cagcccgttc ctctgagata ctaaaggaga tggactctga cgacgccgcc
      841 gacctaatcg cagagcttcc ggaagccaag gctgaaaccc ttttggatct catgaactca
      901 cacggacaag atgtgcggga actgcttcaa tatgatgagc actcctcagg cggcatcatg
      961 gccacagaat acgtgtccat ccagtccgac tggaccgtgg agcagacatt ggcagaactt
     1021 cgacacgtag ctcctgaggc cgaaacagtc tactacattt acgtgctcga tgacgacgaa
     1081 catttggtcg gagtcttgtc ccttagggac ctggtcgtgg cagacctttc cactaggatc
     1141 gacgccatca tgaacactaa cgtgctttca gtgccagtcg aggcggacca ggaagacgtc
     1201 gcccaactgt tcaagaagta tcgatttctg gctcttcccg tcgtcgacaa ggaacaaaga
     1261 ctcacgggca tcattacagc cgacgatatt ctcgacgttt tcgaggaaga ggccacagaa
     1321 gacatgcaga aaatcgcagg aacaatgcct ctggaacgcc cgtatttttc gacgagcatc
     1381 aaagagcttt ggggcagccg tatcgtatgg ctcctggtac tcttcatcgc cgagtctttt
     1441 accggaggca taatgcggca gttcagtgac gcgatgcaaa gcgttataga gctaaccttc
     1501 ttcataccgc ttctgataga ctgcggcgga aatgcaggct ctcaggcatc caccgtggtc
     1561 atacgaggtc ttgcggtaag ggacataaaa cttcgggacg tatggactgt gatggcaagg
     1621 gaacttgtcg tcggactcgg cctcggggta gtcatggctg gcgtaggatt ctttcgggct
     1681 tggcgggttg gaggctcatc ggatattggc attatcgttt gcctggcggt gctagcaata
     1741 gtcgttctga gcaccgtgat aggcgcactt ctgccgataa tagctacagc cctcaaggtg
     1801 gacccggcag tgatgtcggc gccgctgata accacgatcg tggacgcttt cggcctgctg
     1861 atctacttca gcatagccaa atcagtactg caactctgaa agagctcgag aaacaaacga
     1921 gagtataggt gcgccgcccg ccatgcgggc ggccttgtta acgataccat cacctgtatt
     1981 ccgcaaccgc ctcgtcgctt cggaggctct cctgcccttg cgcatctcgc gcaacaacta
     2041 cgaacctcac ttccgagccg cgttgacgcg aagagagcga cactgcagcc gatgatgccg
     2101 gcgcttcaac cacgacagat ctggacgtaa tgtaccgctc ctgagtggaa taacgccagt
     2161 agaccctgta gtggtcgacc atgctctctt ctactggagt ccacgaaacc gtggcagagg
     2221 tgtcctctat gctaacggca acatccttag gcgatattgg atagggctga aagccgattt
     2281 ccacctcaag gctcccgaac cccggcgtcc aactgactgt tgttctcttc cccggacata
     2341 caccaatgcc tatatagtcg ctttcgtaga gggcagatcc atcttccctg agaaatacca
     2401 ctcgcatgtc atatgatcga ggaacgtaac ctgtgtcttc cactgtaaac agcccgcccg
     2461 aacgccgaat atccttgtag gaacgatatg ctgacgtgcc tcctgcgtgg tacacccaaa
     2521 actccgcggc atctatggac tcggactcag gcagcccttc tggtgacgct tcaaacctca
     2581 ctctgcccgg cactgggttc agggtcattt taagtggaac aacagtgtcc cgcctcacct
     2641 cagccttagc cttcccctca tagatcgtgt caccctcccc atccacagca gccgcatcaa
     2701 tattccacac gccggccgca agagactgga taaggcttga tgctgagtgg ttcacaatgg
     2761 gaagctcttc cctgatctca aacccaccca tggtcagcct gagggcgatc actctgggcg
     2821 gacaggcagt cgatggaacc gattttatgg aggcatgcac ggacatagct ccaatgccgg
     2881 cctcagcccg ctgactcttc aggcaaccag acattcctgc cgtcatcagt atcatcacaa
     2941 caccaagtgc aaatcgccgc tgtctacaca ttttcaatcc tcctctcata ttgtcctgag
     3001 cacagcatgc gagctaccga tagacatatt gaaggattgc tgcgtcaaag gcctcgacca
     3061 cacgccacgc tcgcgcaaga gcgaccacgc ggactggtcg gtctacgctc agtactttcc
     3121 tctctttcga cccatgtcct tccataactg caaataccac ccggatgata tttgagccat
     3181 accttggagg tttttcaaac agttcgtcga aagttaacgt ggaataacac gccatttctc
     3241 tgctccaacg ttcgctggca caatagctta ctcttgaggg ggcttagccc ttgatcttgt
     3301 cacgatcgcg aaaacccttt ggcaatataa gctcatcatc gccggagtct tccaccgact
     3361 gggtccagtg cactttttgg gcaatgttat tgtcctcaat catcctgtca accgtcattg
     3421 tcagagcgaa tcatctgtgt cgtgaagtca aaaacaacct tctatctcgt gcaacaaccg
     3481 ctgccgcctt gatagatcct gccattcttt ccaatctgga agaagcgttt agtcttcttc
     3541 tccggccgtg ctccaacagg gttgcatccc ccaaccaaac acgcccaagc ctcaccgctg
     3601 catacatcgt cagcctagac agccagagaa tcaggttcat tagcggcgca tccgctcaac
     3661 cgggttccag caatactctg cctaatcccg gcgatgtaat tggcagagca tctccggaag
     3721 aggtggctct gatttttgcc gggcatccaa ttgtcagagg cccgtacccc agtggtcgca
     3781 gcaaatcggt cacagcgtgt gccccggtaa ttgacactcg cacaggcacc gtggttgctg
     3841 ctctggccct cgaagttgac gcggcagact gcccgaagcc acttgcaaag ggatggtcgg
     3901 tgatcgcctt cgcgctagcc cttacaggaa tactcggcat agcgctgcca cgaatgcacg
     3961 ctgagaacga ggcgaccgaa ca
//
LOCUS       DAOSAI010000232         3981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14305_length_3981_cov_3.686449, whole genome shotgun sequence.
ACCESSION   DAOSAI010000232 DAOSAI010000000
VERSION     DAOSAI010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14305_length_3981_cov_3.686449"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..740
                     /locus_tag="PLM83_07910"
     CDS             141..740
                     /locus_tag="PLM83_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71364.1"
                     /translation="MAKAVLDAFTRQLADEIEAVYAHLESMPAYTVSEILAPPDIRPA
                     MLIGLGAPAPVFIPPAADRLGISWEILPYYEEANAVGAAASQPTAAITLHADTMLGVL
                     TVPEADHVERIKRPITFDIKQARSEAIAWACRFAEEIGVEASQGVSIVEEESFNVVRG
                     FYTAGHIYSIKAQVRPQVRRIKAAETSGVEGPDQSKEDV"
     gene            743..2086
                     /locus_tag="PLM83_07915"
     CDS             743..2086
                     /locus_tag="PLM83_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOK71365.1"
                     /translation="MEQRKTRVGLIFFPAFDWAITPDHPEREERLLYTRDQIIEEGLL
                     DVPGVEEYRPAIATDTDILRAHVCVPTVNRVITESHRIAAGGAMLAADLVLSGEVDRA
                     FALVRPPGHHAMRIVHGSRGFCNINNEAIMVEHIRRTHGPKMRIAIVDTDAHHADGTQ
                     DIYYNDPGVLHICLHQDGRTLYPGTGFIDERGGPRAYGMTVNVPLPPGTGDEGILYVL
                     ENLVLPILKDFEPDVIVNAAGQDNHYTDPLTNMNFTAFGYARLTDLLAPDIVVLEGGY
                     SIEGALPYVNVGILLALAGLDYSAVREPDWDPEIARQPQRVTEEIHRLTGKLQEMWAD
                     REEADIGAIFGKGEYFERGRRIYYDTDNIVEQQREQIRLCPSCSGWRAVFSHALHAST
                     GRTAQIAAMLVPWQACPDCRKAAYSQFELAQKSRAFDRVYLQDPDDDNFLSSRGT"
     gene            2998..>3981
                     /locus_tag="PLM83_07920"
     CDS             2998..>3981
                     /locus_tag="PLM83_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK71366.1"
                     /translation="MSRTTLKDIAEKAGVSVTAVSRALSGYPDISEETRSRILRIAYE
                     LNYQPNTAARTLVTQKSGVMGLFVLGRERGEGFSHPFSGQVINGFLDELTEEGYDMMV
                     FKANRPPDSTNSSYLDLCRRRMVEGAFLMGLRTDDPWLADLTTSTIPIVTIDMPLAGD
                     MTFSVGCDNLSAVHSAIRHLADLGHRRIGIVNGYKQASVSLERLQGYRNALDDLEIPF
                     DPSLVAEADFTAKGGKKAFLSMIDRSPDMTAVFAASDLMAMGVLEGARERGLTVPDDL
                     SVVGFDNIDMAAMSSPPLTTLDQPRYELGQTAADMLISACNGETPPSRVLLK"
BASE COUNT          929 a         1170 c         1084 g          798 t
ORIGIN      
        1 ttccggtcgt tcgcggcggc tcgaagccta cccctactga tgcagcggta gcagcggggc
       61 tttcgtccct tggaagccgc tagctcgcca ccacggccat ggcgcaattg ggcgcaccgt
      121 atggcatgga tgcttcgtcg gtggcaaagg cagtgctcga tgcattcacc cgccagctgg
      181 cagatgagat cgaggctgtt tatgcccatc tcgagtcgat gccggcatat accgtctcgg
      241 agatacttgc gccccccgat atccgccccg ccatgctgat agggctcggc gcaccggcgc
      301 ctgttttcat cccgcccgcg gccgaccgcc tcgggatatc atgggagatt ctgccttact
      361 acgaagaggc taatgccgtt ggcgccgcag cgagccagcc cactgcggca atcacgcttc
      421 atgccgacac aatgctaggc gtacttaccg ttcctgaagc cgaccatgtg gaacgcataa
      481 agcgcccgat aacattcgat atcaaacagg cccgctccga agccatcgca tgggcctgtc
      541 ggttcgccga agagatcggc gtcgaggctt cgcagggcgt ttccatcgtc gaagaggaaa
      601 gcttcaatgt cgtgcgcggc ttctacactg caggccacat atactccata aaagcgcagg
      661 tgcggccaca agttaggcgc atcaaagcag ctgaaacctc aggcgtcgaa ggacctgatc
      721 agtcgaagga ggatgtttag aaatggaaca gcgcaaaact cgtgtaggac tgatcttctt
      781 ccccgccttc gactgggcga taactccgga ccatcccgaa cgcgaggaac ggctcctcta
      841 tacccgcgac cagatcattg aggagggcct cctggatgtg cccggagtgg aagaataccg
      901 tcctgcgatc gcaacagata ccgatattct gcgggcgcac gtgtgtgtcc caactgtgaa
      961 ccgggtgata actgagtccc acagaatcgc tgcaggcggc gccatgctcg cagccgacct
     1021 agtgctgagc ggagaggtgg atcgtgcctt tgcgctagtg cgaccgccag ggcaccatgc
     1081 catgagaatc gtccacggct cacggggatt ctgcaacatc aataacgaag ccataatggt
     1141 agagcacata cgccggaccc atggccccaa gatgcgcatc gccatagtcg acaccgacgc
     1201 ccaccacgcc gacggcacgc aagacatcta ctacaacgac ccaggcgttc tccatatctg
     1261 cttgcatcag gatggacgaa ctctctaccc cggcaccggg ttcatcgacg aaagaggcgg
     1321 ccctcgagcc tacggcatga cggtgaacgt gcccctgcct cccggaaccg gggacgaggg
     1381 aatactgtat gtcctcgaga acctggttct tcccatactc aaggacttcg agccggacgt
     1441 gatcgtcaac gccgcagggc aggacaacca ctataccgat ccgctgacca atatgaactt
     1501 caccgccttc ggctatgccc ggctcaccga tctcttggcc ccggatatcg tagtgcttga
     1561 aggaggatac tccatcgaag gcgcactgcc atacgtgaac gtcggaattc tgctggctct
     1621 ggcgggcctc gattactcag ccgtacgcga gcccgactgg gatcccgaaa tcgcgaggca
     1681 gccgcagcgg gtaactgaag agattcaccg cctcacaggc aaattgcagg agatgtgggc
     1741 tgaccgagaa gaagccgaca tcggcgcgat ctttggcaaa ggagagtatt tcgaacgcgg
     1801 ccgccgcatc tactacgaca ccgacaacat agtcgaacag caacgcgagc agataaggct
     1861 gtgcccctca tgcagcgggt ggagagctgt cttctctcac gcgcttcatg cctctaccgg
     1921 aagaaccgcc cagattgccg cgatgctcgt gccgtggcaa gcatgccctg actgcagaaa
     1981 ggccgcatat agccagtttg agctggcaca gaaaagtcgt gcgttcgacc gcgtctacct
     2041 gcaagatccg gatgatgata actttttgtc cagtcgtggg acttagctac tgcgttgccg
     2101 ctgtttaggg gcacgaacac gagctggtca atccggacgt tggaaaacga atatgcattg
     2161 aaactagtgc gctctgcacg gggttaatcg cccaagtggc caagaaaacg gcgtcaggtc
     2221 acttttcggc atctattgtc tgatgtgccg aatttatcga cacaagccaa tcataggcag
     2281 cggctcaatc caccacaatc ccagaggttt gtcctgtatg tcgccgcatg gcagacgccc
     2341 tgagagaaaa gggcctccgg ctacgaactc ctatagcaaa gccttatggc tacgggccgc
     2401 accgaaggta gcggcgattt cattcatttg aacgcacata cgaatgcgac tgtgcgagcc
     2461 tgaggcgggg gggtatgaag gcccaagccc tgaccgaatg gaccatatcg ggccattcct
     2521 cgctacatac acaaaaagta gacgatgatg ggacagtatt cctcatgtcc ggcagttcag
     2581 cgggactaat caaggcgtag cgcgctcttg ttctctgggc gcagatccgt cggtacttgg
     2641 ggagaaatag tgctacggaa agatgcagaa aacgggtttc ggtttcagtc tgaggttatt
     2701 cgcctatact tcgcccacac ggccgcaccc aaccggccat tccatccccc cccaatctta
     2761 tttcaaaccc aggtaggatc tttcagccct atgtagaata ctgttcatta ggtttaacgt
     2821 ttaagtgatt tcgcccgtgc atcgcagtag cgccttggcg tttaaaaact tggtaacaca
     2881 taaccgcacg caaacgcagt ttttatcgtc aatgttgttg cttaatcgat ttagttccgt
     2941 ggaggcccaa tgccccacat aacaattgcg aacgacaaac gaggaggcca cgaacgcatg
     3001 agtcgcacca cgctaaagga tattgcagaa aaggccggtg tatcagtgac tgccgtctcc
     3061 agagctttga gtgggtaccc agacataagc gaggagacgc ggagccgcat cctccgcatc
     3121 gcatatgagc tgaactacca gccaaacaca gcagcgcgca ccctggtcac tcagaagtcc
     3181 ggggtaatgg ggcttttcgt gttgggcagg gaaaggggcg aaggcttttc tcacccgttt
     3241 tccggacaag tgatcaatgg attccttgat gagcttaccg aggaaggata cgacatgatg
     3301 gtgttcaagg cgaaccgtcc gcccgactca acgaactctt cttacctcga cctctgcagg
     3361 cgacgaatgg tagagggcgc atttctgatg ggactccgca ccgatgatcc atggctcgca
     3421 gaccttacaa catcgacaat tccgatagtc accatcgata tgcccttagc cggagacatg
     3481 acgttctcag tgggatgcga caacctgtca gcggtccatt ccgccatcag gcacctggca
     3541 gatctaggcc acagaaggat cggtatcgtc aatggataca aacaagcctc tgtcagcctc
     3601 gagcggctcc aaggctacag gaacgccctg gacgatctgg agattccatt cgatccctcg
     3661 ctagttgccg aggccgactt cacggccaaa ggaggcaaaa aggccttcct ttccatgata
     3721 gacaggtctc cggacatgac cgctgtattc gcggcatcag acctcatggc catgggcgtg
     3781 ctggaaggcg cccgcgaacg gggacttact gttcctgacg atctatccgt agtcgggttt
     3841 gacaatattg atatggccgc aatgtcctct ccgcccctga ccaccctcga ccaaccccgt
     3901 tacgaactag gccagactgc ggcagacatg ctgatctctg cgtgcaacgg ggaaacaccg
     3961 ccatctcgcg ttctcctcaa g
//
LOCUS       DAOSAI010000233         3941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14501_length_3941_cov_2.678590, whole genome shotgun sequence.
ACCESSION   DAOSAI010000233 DAOSAI010000000
VERSION     DAOSAI010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14501_length_3941_cov_2.678590"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..654)
                     /locus_tag="PLM83_07925"
     CDS             complement(79..654)
                     /locus_tag="PLM83_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK71367.1"
                     /translation="MNDLRERVRAGDRNALEQLFAENIETVSKMASSIAGARCDIEDM
                     VQEALLRAVRSIRNFRWECSFSTWLYRILVNVNKDFARKACSAEIVSLDQLAEQSDDA
                     LLPCVRSEIGQPEEEAIRQEECETLAEAIRALPRLDSSVLWMREALNLSYAEIADNLG
                     CSVESVRSRLWRARRRLRENMALLIPEHVPQ"
     gene            complement(790..2133)
                     /locus_tag="PLM83_07930"
     CDS             complement(790..2133)
                     /locus_tag="PLM83_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOK71368.1"
                     /translation="MNGIHIFHRNRLGASCPKQYVLSAVIILAAIIVASHSACATDAA
                     SPGKTVFVRVGIPCNGQVTLGSGTAGELLQIGEDLKLDHVQFCVGEDGEIACIGPDGA
                     RKDLHAPILVHLAKEVEEAGGQEVALIWLDNAKAVYKGSFELSVDSKGGLSVVNIVAL
                     EDYVAGVVPYEIGASSPYEALRAQAVVARTEALGGLGRHAPQGFDLCATTHCQVYKGA
                     GAELANPMVRQAVDSTSGEVIFYGDVPAKAANYHACCGGFTESPGDLWGTDVPYMHTV
                     RCNGEIDACGDGVYMSEEDVRNWIETPDPKDYCYGSNGYRWSVVYSQEALAEILQPVI
                     GRGVCVESLEVAARTPRGAAVRLVIHTSSGDFEVAGEYAIRLALGGTQSVKSGVFVVN
                     AYGDAPAKFELVGAGYGHGVGMCQYGARAMAKQGYDYKAILEKYYPGATVGAFRP"
     gene            complement(2328..2972)
                     /locus_tag="PLM83_07935"
     CDS             complement(2328..2972)
                     /locus_tag="PLM83_07935"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOK71369.1"
                     /translation="MSDSCILMSRATLARLPFYYRKVLAHAQSGHDRISSAQLAEGLS
                     IDSAQVRRDISSIGDFGRAGIGYNVPELIEALEEILGIKNRTEAVLVGVGRLGSALFR
                     YPGFKDYGLKIVALFDSDPEKVGTELDGTLVRHVSEIPHVTERLSIQMGIITVPSKSA
                     QDVADLLCDAGVIAIWNFAPVGLKTPPHVMVRDEDLAAGLAYLSRHIVVSTSSE"
     gene            complement(2988..>3941)
                     /locus_tag="PLM83_07940"
     CDS             complement(2988..>3941)
                     /locus_tag="PLM83_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOK71370.1"
                     /translation="EATRNEFVSDGRPDVDAVITTQELARMIREAGVRFDDLPVEPFD
                     LPMGMATGAGMIFGNTGGVAEAVLRAASDILEPNKAVGAGSNRRIVFDEVRGVDGVRE
                     ASLTLGGREVRLAVAHSLGAAAQVLERIKSGEAEYDLVEVMACPGGCIGGAGQPNPSH
                     LEVRKKRSKALYDVDTMQMLRRAHDNPFVKQLYDKFLGEPCGETSHELLHTDYGERRR
                     IKGETIKLSSEEVAGQIDVGVCVGTCCYLNGSYDLLKGLMEKVNKAGIEDKVNLHATF
                     CFENCEHSPNIQIDGGIISGVSEDDIERIFREEIMSRLEEQ"
BASE COUNT          921 a         1273 c          889 g          858 t
ORIGIN      
        1 tgtctgtgac aattccgatc ctcgccaaag atgattcccc cctccaaaac agttagacgg
       61 gggaacggga gtaaagtgtc actgcgggac atgctctgga attagcaagg ccatgttctc
      121 ccgcaaccgc ctccgagctc tccatagcct cgaacgtacg ctttcgacag aacacccaag
      181 gttgtcagct atttctgcgt atgacaggtt cagcgcctct ctcatccaca gaacagagct
      241 gtcaaggcgc ggcaaagccc ggattgcttc ggccaaagtt tcgcactctt cttgccgaat
      301 ggcctcctct tcaggctgtc cgatctcgga gcgcacacaa ggtagcagcg catcatcgct
      361 ctgttcggcc agctgatcga gggaaacaat ctcagcagag cacgcctttc gcgcaaaatc
      421 cttgtttacg ttgaccaaga tcctgtaaag ccatgtggag aaagagcatt cccaccgaaa
      481 gttccggata gagcgaacgg ctcggagcag agcctcctga accatgtcct ctatgtcgca
      541 acgggccccg gcgatgctcg atgccatctt cgagaccgtc tcgatgtttt cggcgaaaag
      601 ctgctccagg gcgtttctgt cgccagcacg aacgcgctcg cgcaaatcgt tcattgcccc
      661 atatcacctt caaacgatcg gaaccggccc gcgccaactc ccaaccgaca ttatccggac
      721 atccacccca gactgcgcaa catccaacat gacgcataga atcggctcaa acctagacag
      781 gccatcattt tacggtctaa atgctccaac agtcgcccct ggataatatt tctccaggat
      841 agctttgtaa tcatatccct gcttagccat ggccctcgca ccatactggc acattccgac
      901 cccgtgccca taaccggcgc ccacaagctc gaacttcgca ggagcatccc cgtatgcgtt
      961 caccacgaac actccacttt tgacagactg ggttccgcct agagccaacc ttatcgcgta
     1021 ttcgccagcc acctcgaaat cgcctgagga cgtatggatt accaaccgca cagcagcgcc
     1081 cctcggcgtc ctcgccgcaa cttccaatga ctcaacacat acgccccgcc cgatcaccgg
     1141 ctgcaagatc tcagcaagcg cctcctgaga atagacaacg ctccaccggt agccattcga
     1201 cccgtagcag taatctttgg gatcaggtgt ttcgatccag ttacgaacgt cctcctccga
     1261 catatacacg ccatcaccac aggcatcaat ctctccattg catctcactg tatgcatgta
     1321 tggaacatct gttccccaca aatcgcccgg gctctcagtg aagcccccac agcacgcatg
     1381 ataattcgcc gctttcgcgg gaacatcccc ataaaagata acctctcccg aagtggaatc
     1441 cactgcctgc ctgaccatgg gattggcaag ctctgcgcca gctcccttgt atacctggca
     1501 atgcgtcgtc gcgcaaagat caaaaccttg aggcgcatga cgtcccaagc cgcctaaagc
     1561 ctcagttcgc gccactaccg cctgggctcg caacgcctcg tatggagaag acgccccgat
     1621 ctcatatggc accacgccag caacataatc ctcaagcgca acaatgttga ctacggaaag
     1681 gcctcccttc gaatcgacgg aaagctcgaa tgatcccttg tagacggctt tggcattgtc
     1741 gagccatatg agcgcgacct cctgcccgcc agcctcctct acctcttttg ccaagtggac
     1801 cagaatcgga gcatgcaggt ccttgcgggc cccatcaggc ccaatgcatg ctatctctcc
     1861 atcctcgccc acgcagaatt ggacatggtc aagcttgagg tcttccccta tctgcaggag
     1921 ctctcctgcc gttccagagc ccagtgttac ctggccattg cagggtatac ccactcgcac
     1981 aaacacagtt ttgccgggag atgccgcatc cgttgcacat gccgaatgcg atgctacaat
     2041 gatggctgca agaatgatga ctgccgatag gacgtactgc tttgggcagc tagccccgag
     2101 tcggtttctg tgaaagatgt gaatgccgtt caaatcgggt taacctccat catggccttt
     2161 gagagccgat gaccgcaatc ttaaagcatg cagacacgac cgctcgcgcg tgaccaaggg
     2221 aattgggcct gccaacaatc acagaagaaa cgtttccggg gcaaccgcgc cgtgtgcacg
     2281 attgccccgg aatgtttcca caaactcgct tgtatttcct tctttgacta ttcgctggaa
     2341 gtagagacga ctatgtgcct agacagatat gccaacccag ctgctaaatc ttcatccctg
     2401 accatcacat gcggcggagt cttgagcccc acaggcgcaa agttccagat agctatcaca
     2461 cctgcgtcac acaggagatc ggccacatcc tgggcacttt tgctgggaac agtaattatc
     2521 cccatctgga tcgacaacct ctcggtaaca tgcggtatct ctgacacatg acgcaccaac
     2581 gtgccatcta gctccgtccc tactttctcg ggatccgaat cgaacaaggc cacgatcttg
     2641 agcccatagt ccttgaagcc agggtatctg aaaagtgcag acccgagacg tccgacacca
     2701 accaagaccg cttcagtccg attcttgatc cctagtatct cctcgagcgc ctcgataagc
     2761 tcagggacat tgtagccaat gcccgctctt ccaaaatcgc caattgaaga gatgtcacga
     2821 cgaacctgag cagagtcgat gctaaggcct tcagcaagct gggcagagga gatcctatcg
     2881 tgcccagatt gagcatgcgc caacactttc cgataataga acggcaatct cgccaaagtc
     2941 gcccttgaca ttagaataca tgaatcagac actaaaccac ccctcgccta ttgctcctcg
     3001 aggcgggaca tgatttcctc tctaaatatc ctctcgatgt cgtcttcgct aactccggat
     3061 attatgcctc cgtcaatctg gatattagga ctatgctcgc aattctcgaa acaaaacgtg
     3121 gcatgaaggt ttaccttgtc ttctatcccc gccttgttaa ccttctccat caagcccttg
     3181 agaagatcgt acgatccatt cagatagcag cacgtgccta cgcacacgcc tacatcgatc
     3241 tgaccggcca cttcctctga ggagagcttg atagtctctc cctttatgcg tctgcgctcg
     3301 ccgtaatcgg tgtggagcaa ctcatgcgac gtctcgccac acggttcacc caggaattta
     3361 tcgtatagct gcttcacgaa cggattgtcg tgcgctctcc gcaacatctg catggtatcc
     3421 acgtcatata gggcctttga ccttttcttc ctaacttcca aatgggacgg gttgggttgt
     3481 ccggcgcctc cgatgcaccc tccgggacac gccatgacct caacaaggtc atactcggcc
     3541 tcgcccgact tgatcctttc aagcacctgc gccgccgctc ccagcgagtg ggcgactgca
     3601 agccttacct cgcgtccgcc cagagtcaag ctagcctccc ggacaccgtc gactcctcgc
     3661 acctcatcaa atacgattct cctgttgctg cccgccccaa cagccttgtt gggctctagt
     3721 atatcagaag cggctcggag cacggcctcg gcaaccccgc ctgtattccc gaaaatcatc
     3781 cctgcgcctg tagccatccc catgggaagg tcaaacggct caacaggaag atcatcgaac
     3841 ctgaccccgg cctcccgtat catgcgggca agttcctgtg tggtgatgac agcgtccaca
     3901 tcaggcctgc cgtctgagac aaactcattc ctcgtcgcct c
//
LOCUS       DAOSAI010000234         3911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14646_length_3911_cov_3.146006, whole genome shotgun sequence.
ACCESSION   DAOSAI010000234 DAOSAI010000000
VERSION     DAOSAI010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14646_length_3911_cov_3.146006"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1710)
                     /locus_tag="PLM83_07945"
     CDS             complement(178..1710)
                     /locus_tag="PLM83_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOK71371.1"
                     /translation="MKAVGIDLGTTFSCLAVLNDYGKPEIVPNKEGERITPSVIMFDQ
                     ENQFTVGTIAKNSAVADPGNVVEFVKRQMGNADYVFVHGETEFRPEDLSALIIKKLVT
                     DAEVLLGTELPGAVITVPAYFDDARRQATMDAGEIAGLNVLAIVNEPTAAALAYGMSN
                     PDINQRIIVYDLGGGTFDVTILEVEEGKIRVVATDGDHMLGGKDFDDRIIMHCARKFE
                     EEHGIDLLEDPEALQDLRQRAEAAKKALSTRQSAKVAVSAQGKRHTVELSREQFEEMT
                     ADLLGRSELLLDTVLKSAELTWNDIDQTLLVGGMTRMPAVCELVRKASGKEPTVSINP
                     DEAVAHGAAIYAGIMLAGKGDPDIILTDAGRRLRDTKIEDVTSHSYGILLVDSATGQD
                     INKIMIPKNSPVPAERKETYYVHEDGQRIVEMVVLQGEDSDPANCTEIGEAVLELDGP
                     KPRNYPIEVVYKYDSNMRMDAVITDPQTGASKALQITERGRLSSAEVEEKREAMKDAE
                     VI"
     gene            complement(1739..2410)
                     /locus_tag="PLM83_07950"
     CDS             complement(1739..2410)
                     /locus_tag="PLM83_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71372.1"
                     /translation="MTTAEIHRIWADFDVSMGGRNGLIIHSSMTVRGYNPGAGREMTI
                     IYWFYYTDNSPVRGAIDEYADVAGNACTAEDFTPPYRDSTYDDFIVFMPYDAFGFDRA
                     GYFEAYCNLGIYYGDKLVARRKRVLMFALSSAGGQGQFEDRPREGRGRPDVLPPPLEE
                     RDGHEDVWSQLGIPRSASRNQKEQMLLEQYNTYRARVNHPDLEKRQEAERMMALIARA
                     RQELA"
     gene            complement(2432..>3911)
                     /locus_tag="PLM83_07955"
     CDS             complement(2432..>3911)
                     /locus_tag="PLM83_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOK71373.1"
                     /translation="PIYGKAEIVPNAEGERTTPSVVYFGDDDSVVVGRVAKNAAVADP
                     YNVVEFVKRQMGNADYMFLRDGREYGPEEISAFILKKLVQDAEALLGEGITDAVITVP
                     AYFDDVRRQATLNAAKIAGLNPLRIINEPTAAALAYGMGNNASKQRILVYDLGGGTFD
                     VTIMDVSHGEIRVVATDGDHMLGGNDFDDQIMILVNEAFRKEHGVDLLEDLENQMDLR
                     QRAESAKKTLSTLESAKISVSAAGKRLSHEITRSEFEEASGHLLQRTRLLVESVMASA
                     KASWKDIDTILLVGGSTRMPMVREMLESMSGKAPNQAINPDEAVAIGAAIQAGIIMGQ
                     RGDASILKTTEGRRLANTVVTDVTSHSFGIILMDTMTNELYNQIMIAKNTPIPARIVD
                     RFYTVSHGQEGVDFVVLQGEDRDPENCVAIGETKLLFERPVHKGYPVEVTYEYDANMM
                     IHAYMEDKKTGRRAELHISQRGRLTDSEVAEKKRLMDRMRVD"
BASE COUNT          789 a         1208 c          934 g          980 t
ORIGIN      
        1 ccccgcacat cacgaacgcg gtctgcagca ggcaaggacg aaacctcatt ccgccgccta
       61 cagcagccca gtacattttc tcgaattcgg gatccaggtc actgcaatcc gcaagcgcct
      121 gctcaaccct gcgcacaatg ggaccgagcc gctcctgata aagcgcacgc gtatccatca
      181 gattacctct gcgtccttca tggcttcacg tttttcctca acttcagcac tggaaagcct
      241 tcccctctca gttatctgca acgctttcga ggcccctgtc tgaggatctg taataacagc
      301 atccattctc atgttcgaat catacttata gacgacttct atcggatagt tgcgcggctt
      361 gggcccgtcc aactccagca cagcctcgcc tatctcggtg cagttggcag gatcgctgtc
      421 ctcgccctgc agaaccacca tctccacaat ccgctgcccg tcctcgtgaa cataataggt
      481 ctccttccgt tcggccggaa cgggcgagtt cttggggatc attatcttgt taatatcctg
      541 gcccgtcgca gaatcgacca gaagtatgcc atagctatgg cttgtcacgt cctctatttt
      601 ggtatcgcga agcctacgcc cggcgtcggt caggattatg tccggatctc ccttgcccgc
      661 aagcattatt ccagcataga tagcggcccc gtgggcgact gcctcatccg ggttgatgct
      721 gaccgtgggt tcttttcccg aagccttcct aacaagttcg cacaccgcag gcatcctggt
      781 catcccgccc acaagcaacg tctggtcgat gtcgttccag gtcaactctg ccgatttgag
      841 gaccgtatcg agcaatagct ctgacctgcc caaaaggtct gcggtcatct cctcaaattg
      901 ctccctcgaa agctcgacgg tgtgccgttt cccctgggcg ctcacggcta ccttggcaga
      961 ctggcgagtc gacagcgcct tcttggccgc ctccgctctt tggcgcaggt cctgcagagc
     1021 ctctgggtcc tcgagaaggt cgatcccgtg ctcctcctcg aacttgcgag cgcaatgcat
     1081 tatgatgcga tcgtcaaaat ccttgccccc aagcatgtgg tcgccgtcgg tcgcaacaac
     1141 cctgatcttg ccttcttcaa cctccaggat cgttacgtcg aaagtcccgc ccccgaggtc
     1201 atacacgatg atgcgctggt tgatgtcggg gttgctcatt ccgtaggcga gcgcggccgc
     1261 agtgggctcg ttcacgatcg caagcacatt gagcccggca atctcccccg catccatcgt
     1321 ggcctgtctt cgcgcatcat cgaagtaggc cgggaccgtg attactgctc cgggaagttc
     1381 cgtgccgagc aagacttctg catccgtcac cagtttcttg atgattagcg ctgaaaggtc
     1441 ttccgggcgg aactcggttt cgccgtgaac aaatacatag tctgcatttc ccatctgcct
     1501 cttcacgaac tcgactacat tgccagggtc ggcaacagcg ctgttttttg cgatggtgcc
     1561 cactgtgaac tggttttctt gatcgaacat gatcaccgag ggggtgatgc gctctccctc
     1621 tttgttcggc acaatctcgg gtttgccgta atcattgagc acggcgagac acgaaaaggt
     1681 ggtcccgagg tctataccta ctgccttcat aaatcgtcac tcctccacac caaacaactc
     1741 atgccagttc ctgtctggcc ctggctatca gcgccatcat ccgttcagct tcctgcctct
     1801 tctcaagatc gggatggttg actctggccc gataggtgtt gtactgttcg agcagcatct
     1861 gctccttctg attgcggctg gcagacctgg gaatgcccag ttggctccat acatcttcgt
     1921 gcccatcgcg ctcctctagc ggaggaggaa gcacgtcagg cctcccgcgc ccctcccggg
     1981 ggcggtcctc gaactggccc tgacctcctg cactcgatag cgcgaacatc aacacccttt
     2041 ttctcctggc cacaagcttg tccccatagt agatccccag gttgcagtag gcctcaaagt
     2101 agcctgccct atcgaatcca aatgcgtcat agggcatgaa aacgatgaaa tcatcgtatg
     2161 tgctatcccg ataaggaggg gtgaaatcct cggctgtgca cgcatttcct gcaacatctg
     2221 catattcatc gattgctccg cgcacggggg aattgtctgt atagtaaaac caatagatta
     2281 tggtcatctc acgacctgcg ccgggattgt atcctctcac tgtcattgaa gaatgaataa
     2341 taagtccgtt tctgccgccc atagatacgt caaaatccgc ccaaatccta tgtatttctg
     2401 cagttgtcat ttcccgctct cccgctcgcg gctaatccac tcgcattctg tccatgagcc
     2461 gcttcttttc tgccacttca gaatctgtca gcctgcccct ttgggatatg tgtagttcgg
     2521 cgcgtcttcc tgtcttcttg tcttccatat aggcatgtat catcatgttg gcatcgtact
     2581 catatgtcac ttccaccggg tatcctttgt gcacaggcct ctcgaaaagg agcttagtct
     2641 cgcctatggc cacacagttt tcgggatctc tgtcctctcc ctgaaggacg acaaaatcca
     2701 cgccttcctg cccatgggag accgtgtaga agcggtcgac aatccgcgcc gggataggag
     2761 tgtttttggc tatcattatc tggttataca gttcatttgt catcgtatcc atcagtatga
     2821 tgccgaacga atgagacgtc acatcggtga ccacggtatt tgcaagcctg cgtccctccg
     2881 tagtcttgag gatacttgca tctccacgct gccccatgat gatccccgcc tgaatcgcag
     2941 cacctattgc aacggcctcg tcagggttta tggcctggtt tggcgccttg cccgacatcg
     3001 actcgagcat ctcacggacc atgggcatcc tcgtggaacc accgacaagg agtatggtgt
     3061 ctatatcttt ccatgaggct ttggcgcttg ccatgacaga ctccaccaag agcctcgtcc
     3121 gttggagcaa gtggccggaa gcctcttcaa actcgctccg cgttatctca tgcgacagac
     3181 gcttgcctgc agcactcacg ctgatcttcg cagattcaag agtcgataga gttttcttgg
     3241 cagactcggc gcgctgcctc aggtccatct ggttttcaag atcctccaga agatcaactc
     3301 cgtgttcttt cctgaaagct tcgttgacaa ggatcatgat ctggtcatcg aaatcgttcc
     3361 cgcccaacat gtgatcgcca tcggtggcca ccacccttat ctcaccgtgc gagacatcca
     3421 tgatggtgac gtcgaaggtt cccccgccca gatcgtaaac gaggattctc tgtttggaag
     3481 cgttattgcc catcccatag gccagtgccg ccgcggtagg ctcgtttatt atcctcagtg
     3541 gattgagccc tgcgatcttt gccgcgttga gcgtcgcttg ccttctcaca tcgtcgaaat
     3601 aggctggaac cgtgatcaca gcatcggtga tgccctcgcc gaggagggcc tcagcatcct
     3661 gaacgagctt cttcaagatg aaggccgaga tctcctcagg cccgtactcc cgcccatcac
     3721 ggagaaacat atagtctgca ttccccatct gacgtttgac aaactccacg acattgtacg
     3781 gatctgcaac agcggcattt ttcgccaccc taccgacgac gacagaatca tcatcgccga
     3841 aatagacaac ggaaggcgtc gtccgttcgc cttctgcatt tggcacgatc tctgcctttc
     3901 catagatcgg a
//
LOCUS       DAOSAI010000235         3902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14699_length_3902_cov_3.633741, whole genome shotgun sequence.
ACCESSION   DAOSAI010000235 DAOSAI010000000
VERSION     DAOSAI010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14699_length_3902_cov_3.633741"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /gene="rplD"
                     /locus_tag="PLM83_07960"
     CDS             complement(<1..267)
                     /gene="rplD"
                     /locus_tag="PLM83_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOK71374.1"
                     /translation="MPKAPLFDARGNRLGEVELPEAVFGIEPNEHAVHEAVTLQLASR
                     RSGTHSTKTISEVRGGGRKPWRQKGTGRARHGSRRSPIWVGGGVV"
     gene            complement(281..916)
                     /gene="rplC"
                     /locus_tag="PLM83_07965"
     CDS             complement(281..916)
                     /gene="rplC"
                     /locus_tag="PLM83_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOK71375.1"
                     /translation="MGRAIIGRKIGMTQIFEEDGRVHPVTVIEAGPCCVVQKKHTATD
                     GYEAVQLGFGEIDKRRVSKPMAGHYAKANVKPSRHLKEFRIESADAYELGQEIKVDQW
                     SVGDLVDVTGISRGKGFAGPMKRWNFSRGPMAHGSHYHRGVGSLGATAPARVFKGRKM
                     AGRLGGTKATSLGLEVVKVDPERNLLMVRGSIPGATNGLVTIRDSVKAKAK"
     gene            complement(937..1245)
                     /gene="rpsJ"
                     /locus_tag="PLM83_07970"
     CDS             complement(937..1245)
                     /gene="rpsJ"
                     /locus_tag="PLM83_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOK71376.1"
                     /translation="MATQKIRIKLKAFDHKILDQSAERIVETAKRMGSEVSGPIPLPT
                     ERNVYTVLRSVHVNKDSREQFEMRTHKRLIDILDPTPKTIDALMRLDLPAGVDIEIKL
                     "
     gene            complement(1410..2612)
                     /gene="tuf"
                     /locus_tag="PLM83_07975"
     CDS             complement(1410..2612)
                     /gene="tuf"
                     /locus_tag="PLM83_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOK71377.1"
                     /translation="MAKQKFERTKPHLNIGTIGHVDHGKTTLTSALTNVLAKNGLAQF
                     MDFDKIDKAPEEKARGITISTSHVEYETPNRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPSIVVALNKVDMVDDPELLELVELEVRDLLSE
                     YEFPGDEIPVIPLSAYKAGECGCGQKDCQWCGPIWKLVEAIDAYFPEPQREIDKPFLM
                     PIEDVFTITGRGTVVTGRVERGVVKVGDEVQIVGLMPQPRKTVVTGVEMFRKLLDQGV
                     AGDNVGCLLRGIDRDEVERGQVLAKPGSITPHTKFTAQVYVLTKEEGGRHTPFFNGYR
                     PQFYFRTTDVTGTVQLPEGTEMVMPGDNTVMTISLITPIAMEPGLRFAIREGGRTVGA
                     GAVASIIE"
     gene            complement(2637..>3902)
                     /locus_tag="PLM83_07980"
     CDS             complement(2637..>3902)
                     /locus_tag="PLM83_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOK71378.1"
                     /translation="VDYLPSPMDVPPIVGVDPTNDEEQIERKPSDDEPFCGLAFKIAT
                     DPYVGKLAYFRVYSGVAKVGDAIYNASKGKRERFGRILRMHANHREELDLVSTGDIVA
                     AVGLRNTTTGDTLCDERAPVVLERMEFPEPVIAVAVEPKSKNDEDRLTVSLAKLAEED
                     PTFRVRTDPETGQTLISGMGELHLEVIVDRLLREFNVQANVGKPQVAYRETITCPVRH
                     EGRFVRQTGGRGQYGHVVLELEPGAQGSGIVFENKVVGGAIPKDFIPAVEAGIMEAAG
                     NGVLAGYPVVDVAVALVDGSYHEVDSSELSFKIAGSLAFKEAMERAKPVLLEPIMKVE
                     VETPELYVGDIMADLGARRGKVTALENVGQNQVIRAQVPLAEMFGYATTMRSLSQGRA
                     NHTMHFLRYEQVPDNLAAEIMRRYRGVLS"
BASE COUNT          788 a         1233 c          941 g          940 t
ORIGIN      
        1 cactactccg cccccaaccc atatgggcga cctgcgcgat ccgtgtctcg cccttccggt
       61 ccccttctgg cgccacggct ttcgaccgcc gccccggacc tcggagatgg tcttcgtgct
      121 atgcgtgccc gaccttctgc tggcaagctg cagcgtaacc gcttcatgca ctgcgtgctc
      181 gttgggctct attccgaaga cggcttcagg aagctctacc tctccgagcc tattgcctct
      241 tgcatcgaac aatggtgctt tgggcatctc ctgcgcctcc ttactttgcc ttcgccttta
      301 cagagtcccg gatagtcaca agaccattag tggcaccggg gattgaaccc cgcaccatga
      361 gcaggttcct ctccgggtcg acctttacga cttctagccc caggctcgta gctttcgtgc
      421 caccgagcct gcccgccatc ttgcgtcctt taaaaacccg agccggagcg gtcgcaccca
      481 gtgatccaac tccccgatga tagtgggaac cgtgcgccat cgggccgcgg ctgaaattcc
      541 acctcttcat cggtccggca aaccccttgc cccgagagat gccagtcacg tctacaaggt
      601 cgcccactga ccactgatcg accttgatct cctggccaag ctcataggca tccgctgact
      661 ctatgcgaaa ctctttcaga tgtctgctag gcttcacatt cgccttagca taatggccgg
      721 ccataggctt gctcacacgc cgcttgtcga tttcgccgaa gccaagctga accgcttcgt
      781 agccatcggt ggcggtgtgc ttcttttgaa caacgcaaca tggccctgcc tctataactg
      841 taaccggatg aaccctgccg tcttcttcga aaatctgggt catcccaatc tttctgccga
      901 ttatagctct gcccacgcgt cacaccccct cgtggcctac aacttgatct ctatgtccac
      961 tcccgccgga aggtcgagac gcatcagagc gtcgatcgtc ttgggagtag gatctaggat
     1021 atcaatcaac ctcttgtgag tcctcatctc gaactgttcg cgagaatcct tgttcacgtg
     1081 aacagaacga agcactgtgt atacgtttct ctccgtcggg agcgggatag gccctgagac
     1141 ctctgacccc attctcttag cagtctccac aattctctcg gcggactgat ccaatatctt
     1201 gtgatcaaaa gctttcagct tgatccggat tttctgagtt gccaaattgg ctccctccta
     1261 ttcgcccgat tcctttgacg gacatactcc acggaagtta ctccggcagc ttcccgcaca
     1321 gacaatctcc cgcttcatcg cagattggtg ggggcgggac ttcccgcccc cacgttgccg
     1381 cattattcac tgtccgaact gcggtcggct tactcgataa tggaagccac tgctcccgcg
     1441 ccaacagtgc ggccgccctc gcggatagca aaacggagcc caggctccat ggcgattgga
     1501 gtaatgagac tgattgtcat caccgtgttg tcgccaggca tcaccatctc cgtgccctca
     1561 ggcagctgta cggttccggt aacatccgtt gttctgaagt aaaactgcgg acgatagccg
     1621 ttgaagaacg gagtatgcct accgccctcc tccttagtca gaacgtaaac ctgagcagtg
     1681 aactttgtat gaggagtgat ggaccctggc ttcgccagca cctggccgcg ctctacctca
     1741 tctctgtcaa tacctcgaag gagacatcca acgttgtcgc ctgcaacacc ctggtcaaga
     1801 agcttgcgga acatctctac gcctgtgacc actgtcttcc tcggctgagg catcaaaccg
     1861 acgatctgca cctcgtctcc gaccttgaca actccacgct cgactcggcc cgtaacaacg
     1921 gtgccgcgcc cagtaatcgt gaaaacatcc tcgatcggca tgaggaacgg cttatcgatt
     1981 tcacgctgcg gctctgggaa ataggcatcg atagcttcaa cgagcttcca aatcggaccg
     2041 caccactggc aatccttttg tccgcacccg cactcgccgg ccttgtacgc cgacagggga
     2101 attacaggaa tctcgtctcc tgggaactca tactcgctga gcaaatccct tacctctagt
     2161 tcaaccagct caaggagttc cgggtcatcg accatgtcca ccttgttcag cgcaaccaca
     2221 attgagggaa cgccgacctg tcgcgcaagc aggatgtgct cacgggtctg gggcatcggg
     2281 ccgtcagcgg ccgacaccac gagaatggcc ccgtccatct gagctgcacc ggtaatcatg
     2341 ttcttgatgt agtccgcgtg tccggggcag tcaacgtggg catagtgacg gtttggcgtc
     2401 tcatactcca cgtgggacgt gctgatagtg attccacgag ccttctcctc gggcgccttg
     2461 tcgatcttgt caaagtccat gaactgggca agcccattct tcgccaggac attcgtgagc
     2521 gccgacgtca gcgtcgtttt gccgtggtcg acgtgcccta ttgtcccgat gttaaggtgg
     2581 ggcttggtcc tttcaaactt ctgtttggcc atttctttca atcctccctt gcattctcag
     2641 gacaaaactc ctcggtatcg tcgcatgatc tccgcagcca agttgtcggg gacctgctca
     2701 tacctgagaa aatgcattgt atggttagcc cttccctggg aaagtgacct catggttgtg
     2761 gcataaccaa acatttccgc gagcggaacc tgcgccctta tgacttggtt ctggccgacg
     2821 ttctccaggg cagtgacctt gccccttcgc gcgccgagat ccgccatgat gtcaccgacg
     2881 taaagctcgg gcgtttctac ctctactttc ataatcggtt ccagaagcac tggtttggca
     2941 cgttccatgg cttctttgaa agccaaagac cctgcaatct tgaacgaaag ctcagacgaa
     3001 tcgacctcgt ggtaagagcc atccacaaga gctaccgcca cgtcaacgac tgggtatcct
     3061 gccaagactc cattgcctgc tgcctccatg ataccggctt cgactgcggg tatgaagtcc
     3121 ttaggaatcg ctccgcctac aaccttgttt tcaaagacaa tgcctgatcc ttgggcgccc
     3181 ggttccagtt ccaaaacgac gtggccgtat tgtccgcgtc cacccgtttg ccttacaaat
     3241 cttccttcgt gtctcaccgg acacgttatt gtttccctgt atgcaacctg aggtttccca
     3301 acattcgcct gcacgttaaa ctcacggaga agcctatcga ctatgacctc gagatgcagc
     3361 tcgcccattc ccgagatgag cgtctgaccc gtctccggat cggttcgaac tcggaatgtg
     3421 ggatcctcct ctgcaagttt cgctagggaa actgtcaaac gatcctcatc attcttgctc
     3481 tttggttcga ccgcaaccgc tatcacgggc tcagggaact ccatgcgctc caacacaacc
     3541 ggtgcacgct cgtcacagag ggtatctccg gtggttgtgt ttctcaatcc caccgccgca
     3601 acgatgtcac ctgtggatac gagatcaagt tcctccctgt gattggcatg cattcttagt
     3661 atgcgaccga atctctcgcg ttttcccttc gaggcattgt agatcgcatc tccaaccttc
     3721 gcaacccctg aatagacccg aaagtaggca agcttgccca cgtatgggtc ggtcgcgatc
     3781 ttaaacgcga gcccacagaa gggctcgtca tcagacggct ttcgctcaat ctgttcttca
     3841 tcgttggtcg gatctactcc gactatgggc ggtacgtcca tcggcgacgg aagatagtcg
     3901 ac
//
LOCUS       DAOSAI010000236         3884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14792_length_3884_cov_3.164795, whole genome shotgun sequence.
ACCESSION   DAOSAI010000236 DAOSAI010000000
VERSION     DAOSAI010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14792_length_3884_cov_3.164795"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLM83_07985"
     CDS             complement(<1..686)
                     /locus_tag="PLM83_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK71379.1"
                     /translation="MSILPLKAGWPVSATEELDYNKAWSRVLYKEKYGVGEMKVQNDE
                     KELLLKNGQILAIDEPNLQGDAILVRNGRIAYVGAEEGAFSRCHGRPEVIDLEGRAVA
                     PGFVESHMHPSILAGMMLDVDCTYPSVKSVADIVDKIAERVDETPKGGWVRGWGYDDS
                     KLEERVHPTRWDLDKVSPDNPVYIRRTCVHMGVANSLALRLSGITADTPDPEGGRIDR
                     DPATGEPTGL"
     gene            830..1081
                     /locus_tag="PLM83_07990"
     CDS             830..1081
                     /locus_tag="PLM83_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71380.1"
                     /translation="MKRLSRLLALFLVVLLSLSLVGCGKKEEPEEDILDNDFEETETW
                     DYDEDDTESTDFDSDSWDFDWEDTTDEEDDDYDWGGSFE"
     gene            1284..2180
                     /locus_tag="PLM83_07995"
     CDS             1284..2180
                     /locus_tag="PLM83_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK71381.1"
                     /translation="MNCANGYANGNETLRLLTSRASCRDFSDKKIPQETINAVLNAGI
                     HAPTGGNLQPYSIIKVENHDMRIRLAEFCGDQGFIAKAPVNLVFCIDWHRLERWASLE
                     DAPFSATQSFRHFWISFQDTVICAQNICTAADAVGLGSCYIGTVLECLRELRELLELP
                     NGVLPVVLLAIGYPRERPMPRRKLGADVVVHDEVYRDLDAEELLRAYENKYEGFALEA
                     TEERVAILEEVCKAVHGDEFAVECLERVREAGLINAAQYRFGLHYRADKLPDGNEDLV
                     RATEEAGFNWFKRADMSVDDKD"
     gene            complement(2500..3504)
                     /locus_tag="PLM83_08000"
     CDS             complement(2500..3504)
                     /locus_tag="PLM83_08000"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOK71382.1"
                     /translation="MSKGSAEALLRVDNLTKHYPITQGIIRQKQVGAVRAVDGISFEV
                     RRGETLGLVGESGCGKSTTGRLLMRLEEPTEGSIRFDGCDWLSMGKEDLRRARKDMQI
                     VFQDPFSSLNPRMTVGEMVGEPLLVHGIAKGAELERRTAELLGVVGLDRSHAHRYPHE
                     FSGGQRQRIGVARALALNPKLIILDEPVSALDVSVQAQVINLLQDLQQEFGLTYIFIA
                     HDLAVVRHISNRVAVIYLGKIVELAQKDELCSSPLHPYTEALLSAVPIPDPKLRGGRQ
                     RIILEGDVPSPVDPPSGCRFHTRCRLAQDICSEKEPVLEDVGGGHMCACWVRGRTEVA
                     "
     gene            complement(3501..>3884)
                     /locus_tag="PLM83_08005"
     CDS             complement(3501..>3884)
                     /locus_tag="PLM83_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71383.1"
                     /translation="LNRDRNLSMVLITHDLGVIAETADRVVVMYASQIVETGDVMTIF
                     EKPVHPYTAGLLRSVPKLNEETERLHPIDGVVPSPLRMPEGCRFAPRCPNAQPACRRE
                     MPPLRRIAPGHEVRCIRAEKEADQK"
BASE COUNT          889 a         1101 c          982 g          912 t
ORIGIN      
        1 aacaaaccag tgggttcacc agtggccgga tcgcggtcga tccgccctcc ctcaggatca
       61 ggcgtatccg ccgttattcc agacagcctc agcgcgagac tgttggcaac gcccatgtgt
      121 acacaagtcc tccttatata gaccgggttg tcaggcgaaa ccttgtccag atcccatcta
      181 gtcggatgca cgcgttcctc cagtttactg tcgtcgtatc cccacccacg aacccatccg
      241 cccttcgggg tctcatctac gcgttctgca atcttatcga caatatcggc cacagatttc
      301 acagaagggt atgtgcaatc cacatcgagc atcattccgg ccaatatgga cggatgcatg
      361 tgtgattcca caaagcccgg cgcaacggcc cggccttcaa ggtcaataac ctctggtcta
      421 ccatggcatc gcgaaaatgc cccttcttcc gcgccaacgt aagctattct gccgttgcga
      481 acgagtattg cgtcgccctg gaggttcggt tcgtcaatag ctagaatttg tccgtttttc
      541 agcaatagct ctttctcatc gttttgcact ttcatctctc caacaccata cttttccttg
      601 taaagcacgc gactccatgc tttattataa tccaattcct ctgttgcaga gactggccat
      661 cctgctttta gcggtaaaat gctcatcttt ctgaccttcc agacaaaacc ctaggtaata
      721 tgtaaactta gctcttcaca aataagtcca tagtagtatt attagttata gagtaggagg
      781 ctactcctaa tttcgactca ttacacatgt ctggaaaggg aagattgtca tgaaaaggct
      841 gagtaggctg ctcgcattgt tcctggtagt cctgctgtcg ttgtcgctcg tcggctgcgg
      901 caagaaagaa gagcccgaag aggacatcct ggacaacgat tttgaggaaa cggaaacctg
      961 ggactacgac gaagacgaca ctgagtccac agatttcgac agtgactctt gggatttcga
     1021 ctgggaagat acgaccgacg aagaagacga tgattacgat tggggcggat cgttcgagta
     1081 gttcgtccgg taaaaacata ctacggtctt atgttgccgt aatgcagttg gataactgac
     1141 tagatctatt cgatttaggg gcgtcggaag gcgcccctaa aaccttaact gggatcaaat
     1201 gatgcgcagc cttccacgcg gctttcagca tatcttttgc tacgcccgcg ggaacaatag
     1261 tatggctttg aggaggtagg cacatgaact gcgccaatgg ctatgcgaat ggcaacgaaa
     1321 cccttcgtct gcttacgtcg agggcaagct gccgcgattt ttctgacaag aagataccgc
     1381 aagagacgat taatgcagtg ctcaatgccg gaatccatgc gccaaccggc ggcaatctac
     1441 agccatattc cataataaag gtcgaaaatc atgacatgcg catccgcctg gccgaattct
     1501 gcggcgatca gggtttcatt gccaaagccc cggtcaacct agtattctgc attgattggc
     1561 accgactaga acggtgggca tccttggagg acgctccgtt ctccgccaca cagtcgttcc
     1621 gccacttttg gatctcattt caggatactg tcatttgtgc tcaaaacatc tgcaccgccg
     1681 ccgacgccgt cgggctcggc tcgtgttaca ttggaaccgt cctggaatgc cttcgtgaac
     1741 ttcgcgaatt gctcgaactc ccaaatggag tgcttcccgt agtgttgctg gctattgggt
     1801 atccccgaga acgaccaatg ccccgcagga agttgggggc agacgttgta gtgcatgatg
     1861 aggtctaccg ggatctggat gctgaagaat tgctgcgggc ctatgaaaac aagtacgaag
     1921 ggtttgccct tgaagccacc gaagagcgag tagcaattct ggaggaagta tgcaaagcag
     1981 tgcacggcga tgaatttgcg gttgaatgcc tggaacgagt ccgggaagct ggactgataa
     2041 atgcagcgca atacaggttt ggcctgcact accgtgcaga caaactgcct gatgggaatg
     2101 aggaccttgt ccgggccact gaggaagcag ggttcaactg gttcaagcgt gctgacatgt
     2161 ccgtagatga caaggactga tattaggact ctcgcgtcac atgctagttg tgagatccaa
     2221 gtcctttcac cggcagccca cagcctggca taggtcgtgg gctacggttc gagcgcctaa
     2281 gaatccccaa gtatgcgagt gtcaattgca gcaacctccg aaggctggca tattgacggc
     2341 gacgccggcg cgtcgcttct tttccccagt cctcagagtg gggcgacccg tcgacaggtc
     2401 attcttgcag aagcgacacg cccaactaac ggccaatcca catgagagct ttcatgggat
     2461 tggcgaaact ccgccggaaa cctcagcggc atattcacat catgcaacct ctgtgcggcc
     2521 tcgcacccag caggcgcaca tatgtcctcc gccaacatcc tcgagcaccg gttccttttc
     2581 gctgcatatg tcctgagcca accgacatct cgtatggaac ctgcagccgc tcggggggtc
     2641 aaccgggcta ggaacgtctc cttcaagtat tatcctctgt ctcccgcccc tcagcttcgg
     2701 gtcgggtatc ggcaccgcag atagcagcgc ctccgtatac ggatgcagag ggcttgaaca
     2761 tagttcatcc ttctgcgcca gctctacaat ctttcccagg taaatcacgg ccactctgtt
     2821 gcttatgtgc cgaactacag caaggtcgtg ggcgataaag atatacgtca gcccgaactc
     2881 ttgctgcaga tcctggagca agttgatgac ctgcgcctga acggaaacgt ccagagccga
     2941 tacgggttcg tccaagatta tcagcttggg attgagtgcg agcgccctcg caacgccgat
     3001 tcgctggcgt tgcccgccgg aaaactcgtg cggatacctg tgtgcatgcg atctgtcaag
     3061 cccgacaact cctaaaagct ctgcagtcct ccgctccaat tcggcgcctt ttgcaatccc
     3121 gtggaccagc aagggctcgc caaccatctc acccacggtc atccgagggt tgagagatga
     3181 aaatggatcc tggaagacta tctgcatatc cttgcgcgct ctccgaagat cttccttgcc
     3241 cattgaaagc caatcacagc cgtcgaaacg gatcgatcct tccgtaggct cctcgagccg
     3301 catcagcagc cgcccggtgg ttgacttgcc acatccgctc tcgcctacaa ggcccaaagt
     3361 ctcgcccctt cgaacttcga aggatattcc atcaaccgcc cggactgcgc ctacttgctt
     3421 ctggcgaatt atcccctgag taatcggata gtgctttgtc agattatcta cgcggagcag
     3481 agcttcagct gatcctttgc tcacttctgg tccgcctcct tttctgccct gatacacctt
     3541 acctcgtgcc caggagcgat tctcctcaag ggaggcattt cgcgcctgca ggccggttgg
     3601 gcattaggac atctaggcgc aaaccggcac ccttccggca ttcgcaacgg actcgggacg
     3661 acgccatcga taggatggag ccgttctgtc tcttcattca gcttgggaac tgaacgaagc
     3721 agacctgcgg tgtagggatg caccggtttc tcgaatatcg tcatcacatc gcctgtctcc
     3781 actatctggg atgcgtacat cactactacc ctgtcagccg tttcggcaat cacgcccaga
     3841 tcgtgggtga tgagcaccat cgacaggttg cggtcgcggt tcaa
//
LOCUS       DAOSAI010000237         3876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14830_length_3876_cov_2.924365, whole genome shotgun sequence.
ACCESSION   DAOSAI010000237 DAOSAI010000000
VERSION     DAOSAI010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14830_length_3876_cov_2.924365"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..1394)
                     /locus_tag="PLM83_08010"
     CDS             complement(300..1394)
                     /locus_tag="PLM83_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOK71384.1"
                     /translation="MEREVQNKLEKVRRLMLERGLDAMLLVERSNFAWITGGEAPSVD
                     ETTGRVAACAVVTEDSAYVAADEAEIARLNQNSCLEQKGFKLISRSWYEGLPDPDSFG
                     ARNAVGADVLMSGAIYYGDEVAPLRFKLEPEEIDRYRWLGGQLGKALEEVARCVEPGM
                     TEHKISSLLAVRLVDSGIAPRVTLIATDGRNTSNSQPTPTYSRMEKYAMLTVAGSRWG
                     LSASATRMVCIGPIPDHIRAWHDAAIRIEAAAICATRPGQPVSKCFDAAVRAYEDVGY
                     PDEWKKHHLGGATGYLVRDYLATGSSQETVLENQAFAWNPIVHGIRSEDTIIATKDGP
                     DIITQTGNWPMRSMQGNDGSEIARPDILVR"
     gene            complement(1410..1628)
                     /locus_tag="PLM83_08015"
     CDS             complement(1410..1628)
                     /locus_tag="PLM83_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOK71385.1"
                     /translation="MPIFEYKCAKCGEKFEELVTGCCADVKCPKCGSDEVKKLFSSFG
                     FKSGSTFSSSSGGSCGSCAGGSCGTCSH"
     gene            complement(1644..2429)
                     /locus_tag="PLM83_08020"
     CDS             complement(1644..2429)
                     /locus_tag="PLM83_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOK71386.1"
                     /translation="MIEVGTCGFSYRDWRGVFYPESISDREMLEWYATQFSVVELDFT
                     YYRMPDAKTIGGMSRRTPENFSFCVKAHRSMTHELSRDSNDIAEAFQTFSKAMAPLVS
                     EGKLGCVLCQFPWGFKRTPENSSYVQRLPALLPDIPIVVEFRNVEWVSQSTFDLLKGA
                     GMGFCCVDEPNLRGLFPPLAVYTSPIAYVRFHGRNAKTWWKHNEAWERYNYLYTEAEL
                     LEWAPKIQRLAEATKRAFVLFNNCHAGQAAVNAKTMLSLLGMA"
     gene            complement(2426..2791)
                     /locus_tag="PLM83_08025"
     CDS             complement(2426..2791)
                     /locus_tag="PLM83_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOK71387.1"
                     /translation="MVVPVTDGELLLVRNVRRAGWECPGGQIEQGESPEEAARRELLE
                     EAGALVDDLKPLCWYTVTNGVNSSKGIVYIAYVLAVGKPSDVQEIAEVKSFGKPPAEL
                     SFKDGFIELVFKLMEGSSP"
     gene            complement(2901..>3876)
                     /gene="dinB"
                     /locus_tag="PLM83_08030"
     CDS             complement(2901..>3876)
                     /gene="dinB"
                     /locus_tag="PLM83_08030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOK71388.1"
                     /translation="PMSQAARLCPHAIFVPGNMARYVQVSREIMRVLEAFTPIMQQVS
                     IDEAFLDMTGCEHFYTDALDLGTQIKQAIKDASGLTASVGIAQNKFLAKLASSKSKPD
                     GLLVICPEDVDAFLSPLPVRDVWGVGEKGERSLARYGIETVEDLRLWSKEWLVEQYGK
                     WGAQLYDLARGIDDDPVVADEEQKSFGSESTFDEDIADRYELVQYLAQHARRVGSRLR
                     GAGKLARTVTVKVKFADFSQVTRSKTMAEPFDDYDTIYNTACTLLDEIDMGMAVRLIG
                     LSVSGLSDCRQLSLFGSDNRNCAIDDVLDELSQRYGSKGVIRGRELED"
BASE COUNT          877 a         1146 c          917 g          936 t
ORIGIN      
        1 atatcgtttc tcgggcctct gcgatcgcct acaagcagtg ttggtttgca atgaagccaa
       61 tcaccggtgc aatgaggttg gcgacgctca cagaggctca cagagcgtca gttctgcagt
      121 agatcgccga aggaacaagt ttgcggcaca cacccgggtg tgcgccgcaa aatcctttgc
      181 tcaactgctg cgcggtcatg agcaaaacac aaaatcatta tgagtgcgaa tctacatgag
      241 tcgtgaggct gcaagatgcc gccagaatgg ctttcaggtt aacataaagc caaccaacct
      301 catcttacga ggatatccgg tcgcgctatc tcactgccgt cattgccttg cattgaacgc
      361 atcggccaat tcccggtttg cgttatgatg tcggggccgt ccttcgtggc gatgatcgtg
      421 tcttcgcttc ttatgccatg gacgatcggg ttccaagcga aagcttggtt ttccaggacc
      481 gtctcttgac tggagcctgt tgctaggtag tcccgaacca gatatcccgt ggctccgccc
      541 aagtggtgct ttttccattc gtcaggatag ccaacgtcct cataggcccg gacggcggca
      601 tcaaaacact tcgagactgg ttgacctgga cgcgttgcac agattgcggc tgcttcgatc
      661 cggattgccg catcatgcca tgctctgatg tgatctggga tcggccctat gcaaaccatc
      721 cttgtggcgc tcgcagatag cccccatcta ctgccagcaa ctgttaacat ggcgtatttt
      781 tccatgcggg agtaggtcgg ggtgggttgg ctattgctcg tattccttcc atctgtcgca
      841 ataagtgtaa cccgcggagc gattcctgaa tcaaccagcc gcactgcaag aagagaagag
      901 attttatgtt cagtcatgcc gggttccaca caacgcgcta cttcttccaa agctttgccc
      961 agctgacctc cgagccacct gtatcggtcg atctcctcgg gttcgagttt gaatctgagg
     1021 ggcgcaactt catcgccgta gtagattgcg ccgctcatca ggacatcggc gcctacagcg
     1081 ttcctcgcgc cgaaactgtc tggatcaggc agcccctcat accatgacct ggagatcagc
     1141 ttgaacccct tttgttctag gcacgaattc tggttgagcc ttgcgatttc ggcctcatcc
     1201 gctgctacat acgctgaatc ctcagtaacc acggcgcagg cagctacgcg tccagtcgtc
     1261 tcatccaccg aaggcgcctc gcccccggtg atccaggcga agtttgaacg ctccacgagc
     1321 agcatggcgt ctaggcccct ttctaacatg agccgacgca ctttctccaa tttgttttga
     1381 acttcccgtt ccatgaacat cacccctgct cagtggctac atgtgccaca tgatccacca
     1441 gcgcacgaac cacatgatcc gcctgaagac gacgaaaacg tcgatccgct cttgaagccg
     1501 aaagaggaga agagtttctt aacctcgtcg ctcccacact ttggacattt gacatcggca
     1561 caacaaccgg taactagctc ttcaaatttc tcaccgcatt tggcgcactt atactcgaat
     1621 attggcattt gtgtccctcc tagtcatgcc atgccaagaa gtgacaacat ggtcttggca
     1681 tttaccgcgg cttgcccggc gtggcaatta ttgaacaaaa cgaaagcacg cttcgtggcc
     1741 tctgcgagac gctgtatttt tggcgcccac tcaaggagct ctgcttcggt gtatagataa
     1801 ttataacgtt cccaagcttc attatgtttc caccacgtct tagcatttct cccgtgaaac
     1861 cttacataag caatgggaga cgtatatacg gcaagaggtg gaaagagccc ccgtaggttg
     1921 ggttcatcca cacagcaaaa ccccattccc gcccctttga gcaggtcgaa cgtagactgg
     1981 gatacccatt ccacattcct gaactcgacc acgatgggga tatcaggcag cagtgcagga
     2041 aggcgctgga cataactgct attttcggga gtcctcttaa agccccatgg aaactggcag
     2101 agaacacaac ccagcttgcc ttcactgact aaaggtgcca tcgctttgga gaatgtctgg
     2161 aatgcctctg cgatatcatt gctgtcacga gaaagctcat gagtcatact tcgatgtgcc
     2221 tttacgcaaa agctgaaatt ctcgggagtc ctgcgagaca tgcctccaat ggtcttcgca
     2281 tccggcatgc gatagtatgt gaagtccagc tccaccacgc tgaattgagt tgcataccat
     2341 tcgagcatct cccgatcgga aatgctttct gggtagaaaa cgcctcgcca atccctgtag
     2401 ctgaacccac aagttccaac ctctatcacg gcgaagatcc ctccatcagc ttgaagacaa
     2461 gctctatgaa tccatccttg aacgagagtt ctgccggagg tttcccgaag gacttcacct
     2521 ctgctatttc ttggacatca cttggcttgc ccacagcaag cacataagca atgtatacaa
     2581 tccccttgga actgtttacc ccgttggtca ctgtgtacca gcacagaggc ttcaagtcgt
     2641 ctaccagagc ccccgcttcc tccaacagct cgcgacgggc ggcctcttca gggctttccc
     2701 cctgctctat ttgtcctccg ggacactccc aacctgcgcg gcggacattt cgaaccagaa
     2761 ggagctcccc gtctgttaca ggcacgacca agacactgtc aggttttgac cattcatcct
     2821 caggaggacg accactaaac caaagatctg tatggactcc gggcccgtcc agcttgatga
     2881 agcctctttc gtcggccttg tcagtcttcc aactccctac ctcgaatcac acccttcgac
     2941 ccataccttt gagaaagctc gtccaacacg tcgtcaatcg cacagttccg attgtccgat
     3001 ccgaaaagcg aaagctgccg acaatcggat agcccggata cagataggcc gataagcctg
     3061 acagccatgc ccatatcaat ttcgtccagc aaagtacatg cagtgttgta gatggtatcg
     3121 taatcatcga agggttcggc catggtcttg cttctcgtca cttggctgaa gtctgcaaac
     3181 ttcactttaa ctgtcacggt tcgcgcgagc ttccccgctc cgcgcagcct tgacccaacc
     3241 ctgcgggcat gttgcgccag gtattgcaca agttcgtacc ggtctgcgat atcctcgtcg
     3301 aatgtcgatt cagacccgaa ggatttctgc tcctcatctg caaccactgg gtcatcatcg
     3361 atcccgcgag ccaaatcgta gagctgtgct ccccattttc catactgctc tacaagccat
     3421 tcctttgacc agaggcgaag atcctctacg gtttcaatgc cgtatcgtgc gagagacctc
     3481 tccccctttt cgcccactcc ccatacatct cgtactggga gaggagagag aaacgcgtcc
     3541 acatcttctg gacagatcac caagagcccg tctggcttgg acttcgatga agccagtttg
     3601 gccaagaact tgttttgtgc tatgcctaca gacgccgtaa gccccgatgc atcctttatg
     3661 gcctgtttta tctgcgtgcc cagatccaag gcgtctgtgt agaagtgttc acacccggtc
     3721 atgtcgagaa atgcctcgtc tatggagacc tgttgcatga tgggcgtgaa cgcctctaag
     3781 acacgcatta tttcccttga cacctgcaca tatcgggcca tgttgcccgg cacgaaaatg
     3841 gcgtgagggc acaaccgcgc tgcctgtgac atcggc
//
LOCUS       DAOSAI010000238         3873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14849_length_3873_cov_2.682294, whole genome shotgun sequence.
ACCESSION   DAOSAI010000238 DAOSAI010000000
VERSION     DAOSAI010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14849_length_3873_cov_2.682294"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PLM83_08035"
     CDS             complement(<1..1090)
                     /locus_tag="PLM83_08035"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOK71389.1"
                     /translation="MITLKEFLAREVVPALGCTEPGAVALAAARARQELGGEYIERIS
                     ADVSDSIYKNGMNVGVPGASGETGNSLAAAMGALAGRADLGLEVLRDCTPEDVVAAKR
                     MISEGRVKLNCMPHVCGVYVEVTVESRTHAATCVIEGEHSNIVRVDVDGETVFSKEEA
                     APAGSTLPRDNGNVLADHANEQETDGFLEGIDYRTLLSLADDMGSDEVEYVMRGVEMN
                     MAIAEYGLTSESKSGLGAGKAIWKLMSENKIEKDLAFLIRSYSSAASDARMAGVQLPT
                     MSAAGSGNQGITAIIPVALAGESMGKSREEIARAVTVSLLSTSFVRSRTGRLTPVCGC
                     SVAAGAGAAAGIACLLGGNWMHSARAMQL"
     gene            1351..1893
                     /locus_tag="PLM83_08040"
     CDS             1351..1893
                     /locus_tag="PLM83_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71390.1"
                     /translation="MRRIYNSHDNDRRRVFVTDIIDKVVKGIGTGVDIVGSKTKELWD
                     STRIKSKIGDLKADRHKVMEELGEEAYEMLRKNAFNFDRLYAKAAEITEIDKEIAEAE
                     EALANIREESDGEETPEATEIEESAAEPEPADVSEEEAEEAEESCSDAPKCACGASIV
                     SGARYCIFCGRPVPSGSSCD"
     gene            2020..3438
                     /gene="pyk"
                     /locus_tag="PLM83_08045"
     CDS             2020..3438
                     /gene="pyk"
                     /locus_tag="PLM83_08045"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOK71391.1"
                     /translation="MRKTKIVCTIGPASDDLDTLRSLANAGMDVARLNFSHGVHEEHA
                     ERIRRIRAVAAECGKNIPIMLDTKGPELRIGAFESGSITLAPGDTFIITTQPLLGNSE
                     IVSVNYPGLPILVSQGDKILLDDGQLEMEVRSVKGPAVECEVIVGGVLSDRKRLSVPG
                     RRIDLPTLSSEDKMDLAFGIEHGIDIIAASFIRTASSVQEIKSFTESRGVSLPVIAKI
                     ESREGIENINSILRSADGLMVARGDLGVELPAEDVPIIQKQLIQKCRRAGKPVITATQ
                     MLDSMIRNPHPTRAEVSDVANAVLDGTDAVMLSGETASGKYPVESVRTMARVVERTET
                     AGHFAEAPVQRGGSAAITATDAIGHATVQIATELKAAAIITSTKSGWTARMIAKHRPK
                     TPIVAVATDEQTARILNLVWGVYPVQGHDIATTDEMISTAVAKAQEAGFVREGDTVII
                     TAGVPVGMSGTTNLIKVHEITG"
     gene            complement(3577..>3873)
                     /gene="aspA"
                     /locus_tag="PLM83_08050"
     CDS             complement(3577..>3873)
                     /gene="aspA"
                     /locus_tag="PLM83_08050"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF021900.2"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOK71392.1"
                     /translation="QSADILGNAARTFADKCVVGIEVNRERCLNMVETNVGVVTALVP
                     YLTYQVACDVAKEAQATGKTVRNIVLERGLLSEEQLDHVLSFSHLTSPSGMDGA"
BASE COUNT          939 a         1129 c         1019 g          786 t
ORIGIN      
        1 ctaactgcat ggcgcgggcc gagtgcatcc agttgcctcc caaaaggcac gcaatcccgg
       61 ccgccgcccc agcgcccgca gccacagaac agccgcacac tggcgtgagc ctccccgtcc
      121 tactccgcac gaaacttgtg gaaagcaagc tcactgtgac tgctcgagct atctcctccc
      181 tcgatttgcc catggattca ccggccagcg ccaccgggat tatcgccgtt attccctggt
      241 tgccgcttcc tgccgcgctc atcgtcggca attgaacccc ggccattctc gcatctgagg
      301 ctgcgctcga gtaggaacgg atgaggaagg ccaaatcctt ttcgatcttg ttttcgctca
      361 tgagcttcca tatggctttg cccgctccca gccccgattt ggattcggaa gttaggccat
      421 attcggcgat ggccatattc atttccacgc cacgcatcac atattctacc tcgtcagacc
      481 ccatatcatc ggcaaggctg agcaaagtcc gataatcgat cccctcaaga aagccatcag
      541 tctcttgctc attggcatgg tcagcaagga cattaccgtt atcgcgaggg agcgtcgacc
      601 ccgctggggc ggcttcctct ttgctgaaga cagtctcgcc gtctacgtcc accctgacta
      661 tgttggaatg ctccccttct ataacgcagg tagcagcgtg agtccgcgat tccaccgtca
      721 cctctacata aacaccgcac acgtggggca tgcaattgag tttgacccgg ccctcgctga
      781 tcatgcgttt tgcagctaca acatcttcag gggtgcagtc gcgcagcact tccaatccca
      841 aatcagccct gcctgcaagc gctcccatag cagccgcgag cgaattccct gtttcgccgc
      901 tcgcccccgg cactccgacg ttcatcccgt tcttgtatat gctgtcactg acatcggcgc
      961 ttatgcgctc tatatactcg ccacccaatt cttgtctagc ccgagctgca gccagcgcaa
     1021 ctgcgccagg ctctgtacaa ccgagggccg gaacaacctc tcgggccaag aattccttca
     1081 aagttatcat gcctgctcgc ccgctcccat cttacgatga tcccggcgca cgtctctgga
     1141 aagccctatt ctacaggccc gcgccgtaca gcttatttat tcttgaaacg ctcggtgttt
     1201 ccctccaaat cgcaaggttt gcagtccaac acttttgcag tcatgcaagt cgcatggtcg
     1261 acgtattctg tgatgcacat gatatgggat gcgctgcctg ccaaagggcg caacccgcca
     1321 tgactaacac ggacggagga tatatgtgaa atgaggcgaa tttataactc acatgataac
     1381 gacaggaggc gagtcttcgt gaccgacata atcgataagg tggtaaaagg aataggtaca
     1441 ggtgttgaca tagtaggaag caaaaccaag gaactatggg actccacgcg aatcaaatcg
     1501 aaaatcggcg acctcaaggc ggacaggcat aaggtcatgg aagaattggg cgaggaggca
     1561 tacgagatgc ttcgtaaaaa cgccttcaac ttcgacagac tctatgccaa agcggcagag
     1621 ataaccgaaa tagacaagga aatcgctgaa gcagaagagg ctctggcaaa cattagagaa
     1681 gagtccgatg gtgaagaaac ccctgaggca accgaaatcg aagaatcggc cgccgaacca
     1741 gagcctgctg atgtttctga ggaagaagcg gaagaggctg aagaatcatg ctcagacgct
     1801 ccaaagtgcg cgtgcggtgc atcgatagta tccggtgcgc gttactgcat cttttgcggg
     1861 cgacctgtac cctctggatc cagctgcgac tagcagcctc tggcaagcgg ctactccttt
     1921 ctgtagccaa ctgcacaggg tcgaaaaggt gaacactggc gaatctattt tttgggcaga
     1981 ctgcaaccgg gcaaggtcca tactcaagag gtgtcagtaa tgcggaaaac taaaatcgta
     2041 tgtacaatag ggccggccag cgacgatctg gacacgttgc gaagtttggc aaacgccggc
     2101 atggatgttg cgcggctcaa cttctcccat ggcgtccacg aggaacatgc cgagagaatc
     2161 cgccgcatac gcgcggtggc ggcagaatgc gggaaaaaca tcccgatcat gctcgacacc
     2221 aagggaccgg agttgagaat cggagcattc gaatcaggtt caatcacgct cgctccgggc
     2281 gacacattca tcatcacaac acagccgctt ctggggaatt ccgagatcgt atcggtgaac
     2341 taccccggcc tgcccatttt ggtttcgcag ggcgacaaga ttctgttaga tgacggtcag
     2401 ctggagatgg aagttcgttc cgtaaaaggt cctgcagtcg aatgtgaagt catcgtcggg
     2461 ggagtactca gcgaccgaaa gcggttgtct gtcccagggc gaaggataga ccttccgaca
     2521 ctgtcatcag aggacaagat ggatctggcc ttcggcatag aacacgggat cgacattatt
     2581 gccgcctcct ttatccgaac ggcctcttcc gtgcaggaaa taaagtcttt caccgagtca
     2641 cgcggcgtat cgcttccggt cattgcaaag atcgagagca gagaagggat cgagaacatc
     2701 aactctatcc tcaggtctgc agatgggttg atggtcgccc gtggcgacct cggcgtcgaa
     2761 cttcccgccg aagatgttcc gatcatccag aagcaactga tacagaaatg ccgcagggca
     2821 ggcaagccgg tgattaccgc cactcagatg ctggactcga tgatcagaaa ccctcatccc
     2881 acccgcgccg aagtgagcga tgtggccaat gcagtcctcg acgggactga cgccgtaatg
     2941 ctcagcggcg agactgccag cggaaaatat cctgtcgagt ccgtacgtac catggccaga
     3001 gtcgttgaac gcaccgaaac agccgggcac ttcgccgaag ctccggttca gcgtggcgga
     3061 agcgcagcaa taaccgcaac tgacgcgatc ggccatgcca cggtgcagat tgcaacggaa
     3121 ctgaaagctg cagccatcat cacttcaaca aaatcgggct ggaccgcccg gatgatagca
     3181 aagcacaggc cgaagacccc gatcgtggct gtcgcaaccg acgaacagac tgcaaggatt
     3241 ctcaaccttg tctggggagt ataccccgtg caaggtcacg atattgcaac gacagacgag
     3301 atgatctcga ccgctgtcgc caaggcacag gaagccggat tcgttcgcga gggcgacact
     3361 gtgatcatca cggcaggcgt cccggtgggc atgtcaggaa ccacaaacct gataaaggtc
     3421 cacgagataa cagggtaaaa aaaggggctc cgaagtgcaa tctgcctagc agaggcgttg
     3481 ccgtaagcgc agcgacgaat cgctaacctg ccaggacaac agactgccgt tcggagccct
     3541 cttaagatgt ctttaggctt tcagcctgtg tacaggctac gccccatcca taccgctggg
     3601 gctggtcaaa tggctaaacg acagcacgtg gtcgagctgt tcttccgaaa gcaagccccg
     3661 ttccagcacg atgtttctta ccgtcttccc agtggcctgc gcctccttgg ccacgtcgca
     3721 agctacctgg taagtcaggt acggaaccaa tgcagtcact actccgacat tggtctcaac
     3781 catgttaagg cagcgttccc tgttcacctc gatgccaacc acgcacttat ccgcgaaggt
     3841 tctcgccgcg ttgcccagaa tgtccgcaga ttg
//
LOCUS       DAOSAI010000239         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14924_length_3858_cov_3.416513, whole genome shotgun sequence.
ACCESSION   DAOSAI010000239 DAOSAI010000000
VERSION     DAOSAI010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14924_length_3858_cov_3.416513"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2224
                     /locus_tag="PLM83_08055"
     CDS             <1..2224
                     /locus_tag="PLM83_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71393.1"
                     /translation="GESRVSDSRTRMMARQMALDDAFPTFEQISCFYKRDLWPPSQLL
                     GYNFGALFMKYIEDAYGDRALADIADIYARGFPLGGSFNRSVRKALGVSIAELYDGFI
                     EALRGEFLAEAESIAAEGLTPAIRLTSLGGVVESPSWSKGTGLAAYANYSSIRNGVRL
                     IDPTTGADREAFACIGYDGAFPGLSADGEWIVYSKPQFNAGPYLERDLYVRNLRSGKE
                     TRLTKGERAYYARFSPDGNQVYYARIDGHDGSSALCAVDMATGTTRAIRSFADWRGAI
                     HSFEPSPDGQSIALSIWRPGGYQDIYLMSQDGSNIVQVTSDLAQDCDPTWTPDGRHVL
                     FSSDPDGVYNIYAADVETGEICKVTNVLSGAFFPSVSPDGSLLLFVGYGKDGYDLLLT
                     DLDIASARPAPRKHDPIPDLPPRQAWEYEITPYMPLAHMAPTFWVPNVTAEGVEALVL
                     GVEPVGLRQYVAGVGLDLANGGPTCQLAYMDQQNARFPLSLAMSGGPKGNSQSVAISF
                     PIWGDPSWSGGLTIEYAHAGLTGTHYHLLAADGNLSHIVGRDLLRSTATLQAQAALTS
                     STFAGPWTPEISVTLANSLRLPMTRNNRFEVQAVAGWTGSESYADMLCIGGHDGKYPV
                     RGVEHAYSSGTHIMLGSAEYEFTLAPVEASFGPTSIFIDDLQASVFIDAGSAGPCFTL
                     ENFVASVGMEVGASIVPGYTSGRGLISLGLAKPIVWRGTAPEPQSDFTIYVKLSGTPL
                     "
     gene            complement(2280..2738)
                     /locus_tag="PLM83_08060"
     CDS             complement(2280..2738)
                     /locus_tag="PLM83_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOK71394.1"
                     /translation="MMPEEFFELLRPHAEKVFEETGIPVEIMLAQAALETGWLKNPIR
                     DKYTGKEAFNLFNIKGEGPAGSVMANDIDYIKGKKVHVEAEFRAYNSYEESFADYASL
                     LTEAQRYAPALAVRDDPVAFAWELQRAGYATDPNYANKLISIMQRHMGVT"
     gene            complement(2955..>3858)
                     /locus_tag="PLM83_08065"
     CDS             complement(2955..>3858)
                     /locus_tag="PLM83_08065"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HOK71395.1"
                     /translation="NGKWSVQMNLVVDGAQVSGEYWYDAYGIPLVLDGVNRGGSLIVK
                     ERTADGKVSGEWAFDFNPFVTEWKGTWTSADRTRRYPFSLRLLADMVQTQEVRRGFIR
                     VWLERPLFAEAKSNRSMAVVQEIVDSQLWSSVDEDFTFFPEMYPDYCRAGMYWEHVDH
                     VTIRYASNSIISMMHETYGYAGGAHGMTWYTPINLRLSSSGASGFGLDDVFLKGSGWE
                     KKVSELIIADLKKQKAGWITRGEVTEVGVDLLDRFTVSPAGIDFYFGPYEMGCYAEGS
                     YVSFLPWSALKGFVDTSGPAGFLK"
BASE COUNT          881 a         1195 c          970 g          812 t
ORIGIN      
        1 tggcgagtcc cgcgtatccg attcccgcac gaggatgatg gcgcggcaaa tggcgctcga
       61 cgacgctttt cccaccttcg aacagatcag ctgcttctac aagcgggatc tatggcctcc
      121 gtcacaactc ctggggtaca acttcggggc acttttcatg aaatacatcg aagatgccta
      181 tggcgatcgc gccctggccg acatcgccga catatacgcc agagggttcc cattgggcgg
      241 cagcttcaac cgatccgtcc gcaaagccct gggcgtcagc atagcggagc tgtacgacgg
      301 tttcatcgag gctctccgtg gagagttttt ggccgaggca gaatcgatag ccgcagaagg
      361 gctcaccccc gccatccgac tgacgtccct cggcggagtc gtggaatcac cctcctggtc
      421 gaaaggaaca ggccttgcgg cctacgcaaa ctactcttca atcaggaacg gagtgaggtt
      481 gatcgacccc acaaccggcg cagaccggga ggctttcgcc tgcataggct atgacggcgc
      541 attccccggc ctctctgcag atggcgaatg gatagtctat tcaaaaccac agttcaatgc
      601 aggtccctac ctcgaacgcg acctgtacgt ccgcaacctc agatcaggca aggagacgag
      661 gctcaccaag ggagagcgcg cctactacgc acggttcagc cccgacggca accaagtcta
      721 ctacgccaga atagacggcc acgatggcag ctcagccctg tgcgctgtgg atatggccac
      781 gggaacaacc cgcgccatcc gctcttttgc agattggcgg ggcgcaatac actcattcga
      841 accatccccc gacggccaga gcatagcctt gtcaatatgg agaccagggg gataccaaga
      901 catctacctg atgtcgcagg atgggagcaa tatcgtgcag gtcacgtcag atctggcgca
      961 ggattgcgac ccgacatgga cacccgatgg acgccacgtc ctcttcagct cagaccctga
     1021 tggagtctat aacatatacg cagctgatgt cgaaaccggc gaaatatgca aggtcaccaa
     1081 cgtgctctca ggagcgttct tcccatcagt ctctcctgac ggcagccttc tcctttttgt
     1141 ggggtacggc aaggacggtt acgatctcct cctcacagac ttggacatcg catcggcgcg
     1201 ccccgctccc cggaaacatg accccatccc cgaccttccg cctcgccagg catgggaata
     1261 cgagataacg ccatacatgc cgttggctca catggcgcct actttttggg tcccaaacgt
     1321 cactgcagag ggtgtggagg cgctcgtcct cggcgtcgaa ccggtggggc tcaggcagta
     1381 cgttgccggc gtgggattgg atctggccaa tggcggtccc acctgccaac ttgcctacat
     1441 ggatcagcag aacgcacgtt tcccgctgag cttggcaatg tcaggcggcc cgaaaggcaa
     1501 ctcgcagagc gtcgctatct cttttcctat atggggcgat ccatcctggt caggcgggct
     1561 caccatagaa tatgcccatg caggcctcac aggaacccat tatcatctcc tcgccgccga
     1621 tggaaaccta tcccacattg tgggacgcga tctcctccgg tccacagcca cgctgcaggc
     1681 acaagccgca ctgacctcta gcacatttgc ggggccatgg acaccggaga tatcggtgac
     1741 actggcaaac tccttgaggc tgccgatgac caggaacaac cggtttgaag tccaagccgt
     1801 ggcgggatgg actggttctg aaagttatgc agacatgctt tgcataggag gacatgatgg
     1861 gaagtatccc gtcagaggag tcgaacatgc ctactcatcg ggaacccaca tcatgctcgg
     1921 cagcgctgag tacgaattca cattggcgcc ggttgaggca agcttcgggc cgaccagtat
     1981 cttcatcgat gatcttcagg catctgtctt catcgacgca ggttcagccg gtccgtgttt
     2041 cacattggaa aacttcgtgg ccagcgtggg catggaagtg ggagcttcca tagttccggg
     2101 ctacaccagt ggaaggggac ttatctcttt aggcctggcc aaacctatag tatggcgcgg
     2161 gacggcgccc gaaccacagt ctgatttcac gatctacgtg aaactgtcag gcaccccgct
     2221 atagcaaacc ccgatatcga ggcgcgcgac aggtggccct gctgcgcgcc tgctattggt
     2281 catgttactc ccatgtgacg ctgcataatc gagatgagct tgtttgcgta attgggatca
     2341 gttgcatagc ccgcgcgctg gagctcccag gcaaacgcga ctggatcatc ccttactgcc
     2401 agggccggag cataacgttg cgcctccgta aggagggacg catagtcggc aaatgattct
     2461 tcgtagctgt tgtaggccct gaattcggct tcgacgtgaa ccttcttgcc cttgatgtag
     2521 tcaatatcgt tcgccataac cgaacccgca ggtccctctc cttttatgtt gaaaaggttg
     2581 aacgcctctt ttcccgtata cttgtcgcgg atagggttct tcagccaccc agtctcgaga
     2641 gcagcctgag ccagcatgat ctccaccgga atcccggtct cctcgaaaac cttttcagcg
     2701 tgcggcctga gcaactcgaa aaactcctca ggcatcatgt caccattgca cctccttcag
     2761 cctcatcaca gccaatctat gaaacaagac cgtcaaagtc gactgccatc agcaccaatt
     2821 cgccgaggca tacgcgatca gcacggtctg ccaccccata ctattccatt ttacggcaag
     2881 cgcaccagca gctccacagg cgcaaagctg catcaagaaa ccagcatacg acaacgcctc
     2941 atcccatcgc ttctttattt tagaaaaccc gctggccccg acgtatcaac aaaccccttc
     3001 agagctgacc aaggcaggaa ggaaacatag cttccttcag catagcatcc catttcatac
     3061 gggccaaagt aaaagtcgat tccggcaggg ctcacagtga acctgtcgag caagtccaca
     3121 ccgacctcgg tcacctcgcc acgcgttatc caacccgctt tctgcttctt caagtccgcg
     3181 attatcagct ctgagacctt cttctcccaa ccactgccct tgaggaacac gtcatcgagg
     3241 ccgaatcctg acgcgccgga agacgaaagc cgcaaattga taggcgtata ccacgtcatg
     3301 ccgtgggcgc ctccagcata cccgtacgtc tcatgcatca tgctgatgat actgttcgat
     3361 gcgtacctga tcgttacgtg gtctacgtgt tcccagtaca tgcctgccct gcaatagtcg
     3421 gggtacatct cgggaaaaaa cgtgaaatcc tcatcaactg aggaccagag ctgactgtct
     3481 actatttcct gcaccactgc catgctccta ttagatttgg cttctgcgaa taacgggcgc
     3541 tccaaccaca cgcggataaa tcctcttctg acctcctgag tctgaaccat gtccgccaga
     3601 agccgaaggg agaagggata tctccttgtc cggtcagcac tcgtccaggt tcctttccac
     3661 tcggtgacaa aggggttgaa atcaaaggcc cactctccgg aaaccttgcc atcagcagtt
     3721 ctttccttaa cgatcaacga tccccctcta ttcaccccgt cgagcacgag cggaatgccg
     3781 tatgcatcat accaatactc ccctgacacc tgcgcgccat cgacaaccaa gttcatctgc
     3841 acagaccatt ttccattc
//
LOCUS       DAOSAI010000240         3856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14934_length_3856_cov_2.765588, whole genome shotgun sequence.
ACCESSION   DAOSAI010000240 DAOSAI010000000
VERSION     DAOSAI010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14934_length_3856_cov_2.765588"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLM83_08070"
     CDS             <1..670
                     /locus_tag="PLM83_08070"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOK71396.1"
                     /translation="GVKVDPDRDVVAVDGVIVRPPSEHLYIAVNKPAGFVTTVRDTHG
                     RPTVLDLVPGLDRRVYPVGRLDMESEGLLLLTDDGELAYGITHPSRGVTKTYRVRLAE
                     PILQAQLDAVASGIELEDGWSAPARASMIDSSGRLVEIELREGRKRQIRRMFAAIGNE
                     VVQLVRIAIGCVSLGSLALGASRELTSEEIRGLRELAGGREQAELDGGGFRPDATGKG
                     QSKV"
     gene            639..1070
                     /locus_tag="PLM83_08075"
     CDS             639..1070
                     /locus_tag="PLM83_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71397.1"
                     /translation="MQPVRARVRFDYRGVAVPGRLFIGHKGAAEASAALRDRATANLK
                     YEVEQGIRVEDVDSGHETYVLIDPATDQEVAYSPVYVVVQADSIEDLADFILRDEFRT
                     IEILDPPELTLSPQEIGRLLARANQRLRTMAQLMVRRSERY"
     gene            1190..1582
                     /locus_tag="PLM83_08080"
     CDS             1190..1582
                     /locus_tag="PLM83_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOK71398.1"
                     /translation="MERFSSRSGFTLVETMTAIVLLSLVALMVFAGVQGSLSLSRKGK
                     LKAEGVAILKSVTEDLSTTPATLKDVADKGEKSYPLEAFDFDPSYEARYSVHGTAHGV
                     YRLEVRVIRDGNCIASNEMLIRMPDIGQ"
     gene            1752..2087
                     /locus_tag="PLM83_08085"
     CDS             1752..2087
                     /locus_tag="PLM83_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71399.1"
                     /translation="MSDQIIDGTSTRYRGLREAADVTSDNRLGYTRYTFHFHGSDASY
                     QEHYWAVDAVLYRSRGTEPPEQMGSANSIEVSGPDARGMCTLRLGFTDPGGHEVEHIT
                     SVRLRNHSR"
     gene            2160..3464
                     /locus_tag="PLM83_08090"
     CDS             2160..3464
                     /locus_tag="PLM83_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71400.1"
                     /translation="MVALSPSTDERGSSLVYTLVVVVVLTIAVVSLLQLAAMSFKTGK
                     FEEERQQALAAAESGIAVASVRLVSNDPMPTSAYPSSVPSIDSTAANWIGPGTGPIPK
                     ELLLGDNVSYRVWVSPPVDGRFTVVSEGRCGGRTRVIQQAFSSSTTGLPFLMSATSTQ
                     LFSIILNGSGSLDGDLHTEGKDAWSVVANLSTPANGTLYVRPDADIKGIRSKVGTYFR
                     SVEVDPNTYRYPVPVCPAGLEYRGRFRANGWQPIVITKSGEYDEFIVNGSCEMTVDAS
                     QSDVIIHTKEDFIINGSVNLKIIGDHALKLYVDGNFTWNGSRGGAPPLDPTKFVIYCT
                     GPKNPRSFPPNVTMNGFPNMAAVIYAPRADVVFNGEGEFSGAIVGSSITYNGSGRLAF
                     PTGVYADKLKSINLGEVDGPPRSVPGSWRELPPLGKDKMKSD"
     gene            3487..>3856
                     /locus_tag="PLM83_08095"
     CDS             3487..>3856
                     /locus_tag="PLM83_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK71401.1"
                     /translation="MDVAIIGAGASGLMAAAAAAGAGADVIVYERNRIPGKKLLISGK
                     GRCNITNDSPVVDIVEAFGPGGRFLYSAMSDYGPEELQALLLSLGVECKVEQGKRVFP
                     MSDSAADVQNALYRHATNQGA"
BASE COUNT          924 a          915 c         1140 g          877 t
ORIGIN      
        1 aggagttaag gtagatcctg atcgcgacgt ggtggcagtg gacggggtga tagtgaggcc
       61 cccgtcagaa catctgtaca tagcagttaa caagcccgca ggatttgtga cgacggtccg
      121 agatacccat gggcgcccta ccgttcttga cctcgtgccc ggcctcgacc gcagggtcta
      181 tccagtggga aggctggata tggaatcaga ggggttgctt ctgctaaccg acgatggtga
      241 gcttgcatac ggaatcaccc atccgagccg gggagttacc aaaacttacc gagtaaggct
      301 tgcggagccg atcttacaag ctcagctaga cgctgtagca agcggcattg aattggaaga
      361 tggatggagt gcgccagctc gggcaagcat gattgacagc tccggcaggc ttgtcgagat
      421 cgagttgcgc gagggtcgta aaaggcagat caggcggatg tttgccgcaa tcggcaatga
      481 ggtggttcag cttgtccgca ttgctatcgg gtgcgtatcc ctcggatcgc ttgctcttgg
      541 ggcaagcaga gagctgactt ccgaagagat cagaggtttg agagagcttg cgggcggtcg
      601 tgaacaagcg gaattagatg gaggaggatt tcgcccagat gcaaccggta agggccagag
      661 taaggtttga ttacagggga gttgccgtgc cgggacgact gtttatcggg cataagggcg
      721 ctgcggaagc atctgctgct ttgcgcgacc gcgccaccgc gaacctcaaa tacgaggtgg
      781 agcagggaat cagggtagag gatgtggatt caggccacga aacgtatgtt ctcattgacc
      841 ccgcaaccga tcaagaagta gcctattctc ctgtgtacgt tgtcgttcaa gctgactcca
      901 tcgaagatct cgccgatttc atactaagag acgagtttag aaccatcgaa attctcgatc
      961 ctcctgaatt gactctgtcg cctcaggaaa tcggccgtct cctcgccagg gccaaccaaa
     1021 ggcttaggac aatggcgcag cttatggtga gacggagcga aagatactga tactcacagg
     1081 cttttctgca ttattcagta ttgcaatgag aaggatttgg tcgcttaatg ttcaatattg
     1141 catttagttg agcgcggtct gttgctcctt atggaggagg atactgacca tggagcgttt
     1201 ttcttcccgt agcggattca ctcttgtcga gactatgaca gcaatagtcc ttctatccct
     1261 tgtcgccctc atggtatttg caggggtgca aggctcgctc agtcttagcc ggaaagggaa
     1321 attaaaggct gaaggagtag ccatactcaa gtccgttacc gaggacctgt caacgactcc
     1381 ggcaacctta aaagatgtgg cggacaaagg ggagaaaagt tatcctctgg aggcattcga
     1441 ctttgatcca tcgtatgaag ccagatactc ggtacacggc acagctcatg gggtttatcg
     1501 cctcgaggtg agggtgatac gggatgggaa ttgcatcgcg tccaacgaaa tgcttatcag
     1561 gatgcccgac atcggccaat aggcgaggct tttggcgccg gccagaggct gggatcacac
     1621 ttatcgagct catgatagcg gttagcatca tgggatttgt gctcgtcgga gctttgtcct
     1681 tcattcggct tggcgcttca acgaagacac ttgtcgatag ggatgtacgc tattcgcagg
     1741 agctgtcttt gatgtcagat cagatcatag atggcacgag cactcggtac aggggcttgc
     1801 gagaggctgc agacgtcact tccgataata gactcggcta tacgcgctat acctttcatt
     1861 tccacggttc ggacgcatcg tatcaggaac attattgggc agtcgatgct gtgctctata
     1921 gatcaagagg gactgaaccc cctgagcaaa tgggatccgc aaactcaatt gaagtttcgg
     1981 gccctgatgc caggggcatg tgcactttga ggctcggatt cacagatccg ggaggacatg
     2041 aagtagagca cataacgtca gtgcgactca ggaatcactc acgttgagca aagtcgctga
     2101 taatgcctga atatttagtc aggagagggc tattcggccc agggatgtgg aggtgagcta
     2161 tggtggcctt aagcccgtca accgacgaaa gaggaagtag cctagtatat acgctcgtgg
     2221 ttgtggtcgt tttgaccata gctgttgtca gtctgcttca gcttgctgct atgtctttca
     2281 agacaggcaa gttcgaagag gaacgacagc aggcgttggc cgcggctgag tcggggatcg
     2341 cggtggcgag tgtaaggctc gtctccaacg atccgatgcc aacctcagca tacccttcat
     2401 ctgttccgag catcgacagc acagcagcaa actggattgg gcccggaacc ggtccaattc
     2461 cgaaagaact tctgctcggt gacaacgtgt cgtatagggt atgggtttct ccgcctgtag
     2521 atggccgttt taccgtagtc tcagaaggca ggtgcggtgg aaggaccagg gtgattcagc
     2581 aagcattttc atcatccacc actgggcttc cattcttgat gtctgccact tccacacagc
     2641 tattcagcat aatcctaaac ggctctggca gtctcgatgg cgacctgcac acagagggaa
     2701 aggatgcctg gtccgtggtg gcgaacctgt ctacgccggc caacgggact ctttacgtga
     2761 ggcccgatgc cgacatcaag ggaatacgga gtaaggtggg gacatatttt cgctcagtgg
     2821 aggttgatcc gaatacctac aggtatcccg tgcccgtctg ccctgcaggg ttggaatacc
     2881 gtggcaggtt tcgagccaat ggttggcaac cgattgtgat aaccaagagc ggagagtacg
     2941 acgagttcat agtcaatgga agttgtgaga tgacagtgga tgcgtcccaa tctgacgtca
     3001 taatccacac aaaagaggat ttcattatta acggatccgt caatctgaag atcatcgggg
     3061 accatgcgct aaagctatat gtggacggca atttcacctg gaacggcagc aggggaggtg
     3121 cacctccttt agatcctaca aagttcgtta tctactgcac tgggccaaag aacccgcgta
     3181 gctttccccc taacgttact atgaatgggt tcccgaacat ggcggctgtg atatatgcgc
     3241 ctagagcgga tgtcgtgttt aacggagaag gggaattcag cggagcgatc gtgggaagct
     3301 caattacgta taacggatct ggaaggttag cctttcccac aggcgtttac gctgacaagc
     3361 tgaaaagcat caaccttggc gaggtggatg ggccgcctag atccgtcccc ggttcatggc
     3421 gggaattgcc cccattgggg aaagataaaa tgaaatcgga ttaacggcag aagtggagga
     3481 agcaagttgg atgtagcaat tatcggcgcc ggagcatccg gtctcatggc ggcagccgcc
     3541 gcggccggag ccggcgccga tgtcattgta tatgaacgaa acaggattcc tggaaagaag
     3601 cttttgatat cgggtaaagg cagatgcaac attacaaacg attcgccggt cgtggacata
     3661 gtcgaggctt tcggcccggg cgggcgattc ctctattcgg ccatgtccga ctatgggccc
     3721 gaggaactgc aagcgttgct gttaagcctt ggcgtggagt gcaaggtgga acagggcaag
     3781 agggtctttc ccatgagcga cagtgcagct gacgtgcaaa acgcgctgta ccgccatgct
     3841 acgaatcaag gggctc
//
LOCUS       DAOSAI010000241         3854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14939_length_3854_cov_3.798368, whole genome shotgun sequence.
ACCESSION   DAOSAI010000241 DAOSAI010000000
VERSION     DAOSAI010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14939_length_3854_cov_3.798368"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1841)
                     /locus_tag="PLM83_08100"
     CDS             complement(225..1841)
                     /locus_tag="PLM83_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019095724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOK71402.1"
                     /translation="MALYNVEVRHDGLHKYRMISGNDPYVVQQKAEAQLAQWNEMWEK
                     KQKAVRTQKTCEEQTKEAQEILACIDNTLLHTVSVDDRVNWDSLKDKRAFSNPKPIAP
                     KKESLPTEPLESWPEFAPLLGFFDKISRKARAKKVAAVEQRFKLAHDDWKAEVDRIEA
                     NHEAALGGYERSLEKWESERQAYEAKQAANNAVIDERRKLYEQKDPGAVVDYCDMVLA
                     NSEYPDFFPQEYELDYNPANGMVIVDYALPAPDVIPRVKEVKYVKSRDEFVEVMVSNT
                     DANRRYDLLLYQICLRTVHELFEADTADAVQQVVFNGWVRSIDPATGNEVNGCVLSLQ
                     VDKESFLEIDLARVDPKACFKSLKGVGSSKLHSLTPVAPMIAMDKNDRRFISSYEVAD
                     QLDEGYNLAAMDWEDFEHLIRELFEKEFTASGGEVKVTQASRDGGVDAIAFDPDPIRG
                     GKIVIQAKRYTNTVSVSAVRDLYGAVINEGANKGILVTTSDYGPDAYEFTKGKPLTLL
                     NGGNLLALLEKHGHKARIDLKEAKLLQADR"
     gene            2556..2765
                     /locus_tag="PLM83_08105"
     CDS             2556..2765
                     /locus_tag="PLM83_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71403.1"
                     /translation="MTVNKESSFRGGYVEMATDAAESAAGDNSLDFVATFCHEMGHVV
                     ARDVIPGFAEPYDIHTTGSRSRVRP"
     gene            complement(2781..3257)
                     /locus_tag="PLM83_08110"
     CDS             complement(2781..3257)
                     /locus_tag="PLM83_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71404.1"
                     /translation="MKEIVEEKRKRLGIVLCAIAIVMTVFGLIETNRSRKIVQILDNY
                     HRVDRIAINRGDVEAVVAGDELERYKEAFEPQVILSGDRKTINRVLGDKYLEMRFFIE
                     DELLFDAAIYELTTGSNVPDSFEMGSRRYIMKVGRDYRSLSKSDKGFLLEAVTACD"
     gene            complement(3278..>3854)
                     /locus_tag="PLM83_08115"
     CDS             complement(3278..>3854)
                     /locus_tag="PLM83_08115"
                     /inference="COORDINATES: protein motif:HMM:NF016309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HOK71405.1"
                     /translation="YEYWVFAQVCRGVEKIMGAGPVGADGAEYDPLGAKLRRGIRVRF
                     PGGVEVHYNKGTRGYSGPLRPDVMLLTQSGMWAFDAKFRVDRGSVSGHATKADTLGAM
                     EDTARVDDIDKMHTYRDALTPGLRGAYVVYPGDEVLMYPAPGSSACNGGPGFRDGGPR
                     VAVDGVGAVPARPGSGMTGLFGLLDKILSLR"
BASE COUNT          896 a         1155 c          857 g          946 t
ORIGIN      
        1 ccccagtagt cattcctatt acgtgaccac ttatgaaagc gcacatgccc aaaagacatt
       61 tccatatgtg gcagaactct cctgcattgt tcgctcatgt tagccgcagc tccagaatcc
      121 gaaatgtagg cacccgtcaa aagtctggca cagtcatcct ccagctgccg tccatctcgg
      181 atcaagacgc aaatctgctt gattcacaat atctgccgaa ttcctcacct gtctgcctga
      241 agaagcttgg cttctttcag atcaatccta gccttatgcc cgtgtttctc cagcagggca
      301 aggaggttgc ccccgttaag aagagtcagt ggtttgccct ttgtgaactc atatgcgtca
      361 ggaccatagt ctgatgtggt taccagtatg cccttgttgg ccccttcatt tatcactgcg
      421 ccgtagaggt cacggactgc ggatacggac acagtgttgg tgtagcgctt ggcttggatg
      481 acaatcttgc cgccgcgaat aggatccgga tcgaacgcta tggcgtccac cccaccatca
      541 cgcgaagcct gagtcacctt gacctctccg cccgatgccg tgaactcctt ttcaaatagc
      601 tccctgatta ggtgctcgaa atcctcccag tccattgcag caaggttgta accttcatct
      661 agttggtccg ccacttcata gctggatatg aacctgcggt cgttcttgtc catggcaatc
      721 atcggggcaa ctggcgtcag actgtgcagt ttggagctgc ccacaccttt gagcgacttg
      781 aagcacgcct tgggatccac gcgagcaagg tcaatctcaa ggaatgattc cttgtcaact
      841 tgcaatgaca ggacgcaccc attgacttcg ttgcctgtag caggatctat agatctcacc
      901 cagccattga aaaccacctg ctgcacagca tcggctgtgt cagcctcaaa gagctcgtga
      961 accgtgcgaa gacaaatctg atataacagc aaatcatatc tgcgattagc gtcagtattc
     1021 gacaccatta cctcaacaaa ctcgtctctg gacttgacgt actttacttc cttgacccgc
     1081 gggattacat ccggcgctgg caaagcataa tcaactatga ccatgccatt agccggatta
     1141 tagtcaagct cgtattcctg gggaaagaag tcgggatact ctgaattggc caacaccata
     1201 tcgcagtaat ccacaacagc cccaggatcc ttctgttcat acaacttccg tctctcatcg
     1261 ataactgcat tatttgcagc ctgctttgct tcatacgcct gacgctctga ttcccacttt
     1321 tccagcgaac gctcgtatcc cccaagggca gcctcatgat tagcctctat tctgtcaacc
     1381 tcggccttcc aatcatcatg agccaatttg aaacgctgct cgacagctgc aaccttcttc
     1441 gccctagcct ttctgcttat cttgtcaaag aatccgagga gaggcgcaaa ctcaggccat
     1501 gactcaaggg gctcggtcgg taaggattct ttcttaggag caatcggctt tggattggaa
     1561 aatgctctct tgtccttgag gctatcccag ttgacgcgat catcaacact gaccgtgtgc
     1621 agcaatgtgt tgtcaatgca tgcaaggatc tcttgggcct ctttcgtctg ttcctcgcat
     1681 gtcttctgag ttcttacagc tttctgcttc ttctcccaca tctcattcca ttgggccagt
     1741 tgggcctcgg ccttctgttg aacaacgtaa gggtcgttgc cagagatcat cctgtactta
     1801 tgcaatccat cgtgccggac ctcaacgttg tacagcgcca tacaacacat cctcccattc
     1861 ctgtgtagta cgcaaataac agcaatgcca aacgatctcc aggcgcctat acaagtcagt
     1921 agagtgatgt tgtacataac cattggcctg caacatatag tgaccgttgc agaagctagc
     1981 gccatattcc tggcattaat atgtttgttc gtctctgtcc gtggaattcc ttcaaattct
     2041 gtccagttga tgagggctgc tggaagcgtc tttcggcaag tagaataagg ggacgggaga
     2101 cacaacagac ggcggtcaaa taaagtgtct ggggcgaaaa cggttcgcac cacgcatata
     2161 gtgccaggtc ggatgaatac acccaaggaa ggaaacctgc acagcatcaa gaatggctcc
     2221 cccaacacaa ttcaaacgct cactcgtcga aagcagcgct gtctgagtag agaaactgca
     2281 gatcctagct cctattgaag atggccccgc tggcatactg catgggcaag gcccaactgg
     2341 ccacgcatat cccgttctcc agaaactgaa ccccgattct gatctgggca gaataatggg
     2401 caccctcagc aacgccctaa ctacaggccc agcaacccgc gcccttatgt tggaaaagaa
     2461 gagccgcctt ctagcctcag gcaattctgg tgaccatgca tacctcctca tccccaacag
     2521 acaaggtgga ttcgccagag tcggattctg gctcgatgac ggtgaacaaa gaatcttcat
     2581 tccgtggggg atacgtggaa atggccacag acgctgccga gtctgcagca ggcgacaaca
     2641 gcctcgattt tgtcgccacc ttctgccacg agatgggcca cgtggtcgcc agggacgtta
     2701 ttcccggctt cgcagagccc tacgatatcc acacaacggg ttcccgttcc agggttcggc
     2761 catgagatcg caggccaggc ctaatcacac gcagtcactg cctccagcag gaatccctta
     2821 tcgcttttgc tcaggcttct gtagtctcga ccgaccttca tgatgtatct ccggcttccc
     2881 atttcaaaac tgtccggaac attactgcct gtggtcaatt cgtaaatggc tgcgtcaaac
     2941 aggagttcat cctcgataaa gaatctcatc tctagatact tatcccccaa aaccctgttg
     3001 atcgttttcc tatcccccga caggatgact tggggttcaa aggcttcctt gtatcgctcc
     3061 agctcatccc cggccacgac cgcctccaca tcgcctctgt taatggctat tcggtcaact
     3121 ctgtggtagt tgtcgagaat ctgtactatc ttccgacttc tgttggtctc tatgagtcca
     3181 aatacagtca tgactatagc tatggcacaa aggactatgc ccaggcgttt cctcttctcc
     3241 tccacgatct ctttcatctg cctctctagt gtgagtctta ccgaaggctc aatatcttgt
     3301 ctagcagtcc aaacagcccg gtcatgcccg agcccggcct cgccggcact gctcctaccc
     3361 catctaccgc gacccggggc cccccgtccc taaaacctgg tccgccatta catgcactag
     3421 accccggtgc agggtacatc agcacctcat cgcctggata caccacataa gcccctctca
     3481 aaccaggggt gagcgcatca cggtacgtgt gcattttgtc tatgtcatca accctggctg
     3541 tatcctccat cgcgcccaac gtatccgctt tggtagcatg gcccgaaaca gaacccctgt
     3601 ccacgcggaa cttggcatca aatgcccaca tgcctgattg agtcagaagc atcacatcag
     3661 gacgcaatgg gccggagtat cccctggttc ctttattgta gtgcacttcc actcccccgg
     3721 gaaacctgac acgaattccg cggcggagct ttgcaccaag aggatcgtat tccgccccgt
     3781 ctgccccgac aggccctgcg cccatgatct tctcaacacc tctgcaaacc tgcgcaaaca
     3841 cccagtactc gtat
//
LOCUS       DAOSAI010000242         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_14980_length_3848_cov_3.447667, whole genome shotgun sequence.
ACCESSION   DAOSAI010000242 DAOSAI010000000
VERSION     DAOSAI010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14980_length_3848_cov_3.447667"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PLM83_08120"
     CDS             <1..817
                     /locus_tag="PLM83_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOK71406.1"
                     /translation="KQEGISYIAGAELPCLIVNMVRGGPGLGGIQPAQSDYFQAVKGG
                     GHGDYYTIVYAPASIQEMVDLTMQAFDVAETYRMPVMILGDGVLGQMMEPVEFRDPVP
                     RELPPKDWATTGAKDGRAPRLINSIYLKPDELFKHNLKIAERFEQAKKNEVMCEKANT
                     DDADIVLVAYGTTARVCRSVMDRARAAGLKVGLIRPITVSPFPYDDIAEVARTAKAFL
                     TVEMSLGQMVEDVKVAVYGAAPVHFYGTTGGVIPTAADIMARVEEIARAEVRK"
     gene            814..1563
                     /locus_tag="PLM83_08125"
     CDS             814..1563
                     /locus_tag="PLM83_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK71407.1"
                     /translation="MKQVYSVPKSLQPRSTHYCPGCTHGIVHKLVAEAIDDLEIRDRA
                     IGVAPVGCAVFAYDYFDCDFQQAAHGRAPAVATGIKRAVPDSVVFTYQGDGDLASIGA
                     AEIVHAAMRGERITVIFVNNAIYGMTGGQMAPTTLVGQKATTAPFGRQKEHCGSPLRI
                     SEMLATIPGAAYIARVALSSPGNVAKAKTAIRKAFETQLAGEGFALVECLSTCPTNWG
                     MAPYEAMQWLEENMIPYYPLGEFRTPEGGAE"
     gene            1563..2099
                     /locus_tag="PLM83_08130"
     CDS             1563..2099
                     /locus_tag="PLM83_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOK71408.1"
                     /translation="MLEELIISGFGGQGVMVIGQLLSYAGLLEGKNVSWIPSYGPEMR
                     GGTANCSVVISTDPIGSPIVSEPLSVIVMNRPSLDRFESALRPGGLLVYNSSLIDRAP
                     IRTDVRVIAVPVNDMAAELGSDRVGNMCALGAYLAATRVVSIDSIAESLKEVLPPRRH
                     DLIPLNLKALEAGRELIK"
     gene            complement(2657..3775)
                     /locus_tag="PLM83_08135"
                     /pseudo
     CDS             complement(2657..3775)
                     /locus_tag="PLM83_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
BASE COUNT          873 a          966 c         1137 g          872 t
ORIGIN      
        1 caagcaggaa ggaatatctt acatagccgg agccgagttg ccgtgcctca tagtcaacat
       61 ggtccgggga ggaccggggc tgggcggaat tcagccggct cagagcgact atttccaggc
      121 agtgaagggc ggcggacatg gcgattatta cacaatcgta tatgcccctg catcgataca
      181 ggaaatggtt gacttgacta tgcaggcatt cgatgtggcc gaaacatacc gtatgccggt
      241 gatgatcctt ggagacgggg tgctcggtca gatgatggaa ccagtagagt tccgggatcc
      301 tgtgccaaga gaactcccgc ccaaggactg ggcgactacc ggggcaaagg atggacgcgc
      361 cccacgtctg ataaactcta tctacctgaa acccgatgag ctgttcaaac ataacctcaa
      421 gatagccgaa aggttcgaac aggcgaagaa gaacgaagtg atgtgcgaga aggcgaatac
      481 tgatgatgcc gacatcgtgc tagtggccta cgggaccact gctagggtgt gccggtcagt
      541 catggacagg gctcgcgctg ccggactcaa ggtcggactg atccgtccca tcacggtctc
      601 tccgttcccg tacgatgaca ttgccgaggt tgcccgcact gccaaggctt tcttgacggt
      661 ggagatgagc ctcgggcaga tggtggaaga cgtgaaagtg gcggtgtacg gtgctgctcc
      721 agtccacttt tacggaacta ccggcggcgt gattccaact gccgccgata ttatggcgcg
      781 cgtcgaggag attgccaggg cggaggtgcg gaaatgaagc aagtatattc tgttccaaag
      841 tcactgcaac caaggtcgac gcattactgc cccggttgca cccatgggat agtgcacaaa
      901 ctggttgcgg aggccatcga cgatctcgag atcagggacc gcgccattgg agtggcgccg
      961 gtggggtgcg cagtcttcgc atacgactac ttcgactgcg atttccagca ggcggcacat
     1021 gggcgtgccc cggctgttgc tacaggaata aagcgggcag tgcccgactc ggtcgtattc
     1081 acgtatcagg gtgatggcga tttggcttcg attggagccg ctgaaatagt tcatgccgcg
     1141 atgagaggcg agcgcattac tgtgatattc gtcaacaacg ccatatacgg catgaccggc
     1201 ggccagatgg ctccgactac gttggtgggc cagaaagcca ctaccgcgcc ttttggcaga
     1261 cagaaggagc actgcggcag cccacttcgg atttcagaga tgttggccac gattcccggt
     1321 gcagcataca tcgccagggt tgcactttca tccccgggga atgttgcaaa ggcaaagact
     1381 gctatcagga aggcattcga gacgcaactg gccggcgagg gatttgccct tgtggagtgc
     1441 ttgtctacat gtccgacaaa ctggggaatg gcgccatacg aggcaatgca atggctggag
     1501 gagaacatga taccatacta tccccttgga gagttccgaa ctccggaagg gggggctgaa
     1561 taatgcttga ggaactgatc atatctggat tcggcgggca gggcgtaatg gttattggcc
     1621 agttgctgtc ctatgcaggc ctgctcgaag ggaaaaacgt gtcgtggata ccttcatatg
     1681 ggcctgagat gcgtggtggc accgccaact gctcggttgt gatatcaaca gatccgatag
     1741 gttcgcctat cgtgtcggag ccattatcgg tcatagtcat gaataggcct tcactcgaca
     1801 ggttcgaatc cgcactgagg ccagggggac tgctcgtata caacagttct ctgatagata
     1861 gagctccgat tcgcacggat gtaagggtga ttgcagttcc agtcaacgac atggctgccg
     1921 agcttggatc tgatcgggtg ggcaacatgt gcgcccttgg cgcgtatctt gctgcgacac
     1981 gcgtggtatc aattgattcc atagctgaga gtcttaagga ggtactgcct ccaaggaggc
     2041 atgaccttat accactcaac ctcaaagcgt tggaagctgg aagagaactg attaagtagg
     2101 cgcaaatacc catggatcag gcatttcttg aggagctgtt gtgcctgtgc ggagcctgtt
     2161 ttggaatagt ggcacatcac atcgccgctg aaactcgatg cgcggcaaag atggcaattg
     2221 gtgatcaatt gcgagctcag caagacaatc aaggcggcac tgaacatggt gtagcgcaaa
     2281 atacggtgct cgctatcgat acagtgttag gtatgatgtt gaaggtcaat atagcatgca
     2341 agtagtacga cgcttgaaac atggccggcc tgaggcggga ggatctgtag ccgctatcgg
     2401 gccggctgtg atttgacggt gaaggagcat ttcgcagaaa catacccaac atgggtagag
     2461 catgctcgca gcgcctggca tcaggctggc tacgggttga gaatgccaaa gcttgtagct
     2521 gggcataaat gaacgaggcc ttgcggccag catgccatgg ttgtggcggc acagtgcagc
     2581 ggtagatcat ggcaaaggga cgccggtcag tggcgtccct cgggatcagg ttagttttgg
     2641 gtcatttcgg ccgcgatcac gggaaaatgc cccgcatttt gatggcgtcc gctacccttt
     2701 tcactccgag aatataggcg cctgtacgga gactgagatc ttcttcatga gcgaaatccc
     2761 agacttctgc gaagctatgg gtcataatct tgtctagcct cgagttgact tcttcctcag
     2821 tccagtagaa gttctagaga ttctgcaccc actcgaagta ggaacagatg actccgcctg
     2881 cgtttgcaag gatatccggg acaacgagga cgccgtttgc cgcgaggatc ttgtcggcct
     2941 cggcagtagt gggcccgttt gcgccttcaa cgataaactt tgctctgacg tcttctgcgt
     3001 tttcctcagt gatctgattc tgcaatgcgg ccgggataag aagatcgacg tcgagggtga
     3061 gaaggtcagc atttgatata ttgctgactc ccggttcgct gtagccttca agaactctat
     3121 tctcctcgac atacttggtt atgtgaggta tgttgagtcc tcccgggttg tagtgtccgc
     3181 cgctaatgtc gctcacggct actaccttgc aacccatttc gtgggctaag gtagcggcaa
     3241 ctgaaccgac atttccgtag ccttgcactg cacatgtgat ctcagatggc ttcttattga
     3301 gccgaagcag caactcgcgg acggcgaaga ccacaccgcg tccagtggct tcgcgtcgcc
     3361 caattgaccc gccgatcggg actggcttgc ctgtgactac acccggactg tagactccgt
     3421 taaggacgga ataagtatcg acgaaccagc ccatgatctg ggcgtttgtt cccatgtccg
     3481 gagcgggtat gtctttttca ggtcctataa tggggtagat agcgtagaca taacgtcgtg
     3541 tcaatcgttt tagctcgttc tctgaaagtt gtgtaggatc aacgcgaatg tcgcctttgc
     3601 cgcccccata agggattccg gcgacagcgc atttccaggt catccacgaa gagagagcct
     3661 tcacttcgtc catgcatacg tccatggcat aacgaactcc accttttgcg ggaccgcgta
     3721 cggtggagtg ctggcacctg tatccagtaa ataccttgat gctcccgtcg tccatttcga
     3781 tgggaagcgc aacctcgagg gtgcgctctg gttctgcgag gatctcgaat atgccgggct
     3841 caagattg
//
LOCUS       DAOSAI010000243         3834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15063_length_3834_cov_3.210373, whole genome shotgun sequence.
ACCESSION   DAOSAI010000243 DAOSAI010000000
VERSION     DAOSAI010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15063_length_3834_cov_3.210373"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /gene="spoVFB"
                     /locus_tag="PLM83_08140"
                     /gene_synonym="dpaB"
     CDS             <1..233
                     /gene="spoVFB"
                     /locus_tag="PLM83_08140"
                     /gene_synonym="dpaB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570813.1"
                     /note="involved in production of dipicolinic acid
                     (pyridine-2,6-dicarboxylic acid, DPA) which is synthesized
                     late in sporulation in the mother cell and accumulates in
                     the spore; mutations in this gene result in a lack of DPA
                     synthesis; presumably functions with SpoVFA to form the
                     synthase enzyme; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HOK71409.1"
                     /translation="IGIATNDGLSGCARNIGMLHSRKNVYFIPYGQDDPIGKPTSIVS
                     RMSEMERTVEEALAGRQVQPLLVEKWKDLRRS"
     gene            353..1378
                     /locus_tag="PLM83_08145"
     CDS             353..1378
                     /locus_tag="PLM83_08145"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOK71410.1"
                     /translation="MLRVAVVGATGAVGREIVSAIFELEFPVSDLILASSPRSAGSKV
                     STPMGEITVTNTEISALEGADVAFLAAGASVSRKLAPELARRGTLVIDNSSAFRMDPE
                     VPLVVPEVNIDAVHEHRGIIANPNCSTIIAVVAVAPIYRRWGLERMIVSTYQAVSGAG
                     HAAIEALADESEAVLAGLDPVPSVLPYKSAEVHHQIAFNVIPHVDSFCEDGYTREEYK
                     MTDEIRKILQDPSLKISATCVRVPVFRSHSESINLQTCQRAPMDEVRAVLASSPGIRL
                     MDDPVSFAYPMPIHVSGKDDVEVGRLREDPSAPNAMWLWVVGDQLRKGAASNAVQIGL
                     ALYDDRR"
     gene            1384..2601
                     /gene="dapG"
                     /locus_tag="PLM83_08150"
     CDS             1384..2601
                     /gene="dapG"
                     /locus_tag="PLM83_08150"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOK71411.1"
                     /translation="MGIIVQKFGGTSVADAQACALVVSRIAEARRAGNDVVAVVSAMG
                     RKPAPYSTDALLALVDCAGDTLPPRERDAIAACGEIISSVVLVSALVASGCPAMSFTG
                     AQAGIETDDNFGDARIVRIDPAGLHNAIASGVVPVVAGFQGVTDGGDVTTLGRGGSDT
                     TAAALAAALGADLVEIYTDVEGVMTADPHIVPNARFQETMTYSELIEMANLGAKVVHL
                     RAVEIASQARIPLAIKSVFSDCPGTLVTDIPPQRMPIAVPDRFVSSVTCIGNMAQIVL
                     EEYDFKADDAALNVFSTMADNHISVDMINVFPDMIAFIVSARDSARAVRALDRLGLSA
                     VANPGCAKVSVVGEGMRGVPGVMSRVVRALHGAGIRILQTSDSHYSISCLVPEVDMER
                     AARALHSEFELEK"
     gene            2634..3527
                     /gene="dapA"
                     /locus_tag="PLM83_08155"
     CDS             2634..3527
                     /gene="dapA"
                     /locus_tag="PLM83_08155"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOK71412.1"
                     /translation="MKKNGRRIRVITAMATPFAPDLSVDYRKARELARRLIENGSDGL
                     VVSGTTGESPTLTHEEKLELFRVVLEEVGGEAAVIAGTGGNDTRTSIELTQEAEKLGV
                     DGAMLVTPYYNKPPQAGLYRHFASVARSTSLPLMIYNVPGRTSVNLSADTMAKLANDF
                     SNIVAVKEASGSLEQAAEIRSKAPKGFTIYSGDDNMTIPMMSVGGDGVVSVASHVAGP
                     GIQQMVTAFIDGDVELAAEMHRRLLPLFKVIFITTNPIPIKAALKLAGFDAGGLRPPL
                     VEATDAELGQIEAVMESLGLI"
BASE COUNT          841 a          935 c         1187 g          871 t
ORIGIN      
        1 taatcggaat tgcaacaaat gatggcctgt cagggtgcgc acgcaacata ggcatgcttc
       61 attcgcggaa aaatgtatat tttataccat atgggcagga tgatccgatt gggaagccga
      121 cttcgattgt gtcgcgcatg agcgagatgg agaggacagt ggaggaagct ttggctggta
      181 gacaagtcca acctttgctc gtcgaaaagt ggaaggactt gcgccgttcg tagcaggaat
      241 tccaggggct gtcgccaata agtatgccta atcggcacgc gccaggcagc ttcatctgcc
      301 tgaagtcatt tttgatgtgc atgttcatac aagttgtggg ggttgatgcc agttgttgag
      361 agtagcagtt gtgggcgcaa caggtgccgt cggcagagag atagtctctg ccatattcga
      421 gttggaattt cccgtgtcag atttgattct tgcatcgtct cccagatcag cggggagcaa
      481 ggtttcgacg cccatgggcg agatcacggt gacaaatact gagatctctg ccctggaggg
      541 agcagacgtt gcgttccttg ccgcgggtgc cagcgtgagc cggaaattgg cgccggagct
      601 ggcgcgccgg ggtacgctcg tcatcgataa ctcgagtgct ttcaggatgg atcccgaggt
      661 tccccttgtg gttcctgaag tcaacatcga tgctgtccac gagcatcgcg gcataattgc
      721 gaacccgaac tgttcgacca taatagcagt ggttgcagtg gccccaatat accgccgttg
      781 gggattggag cgtatgatcg tgtcgaccta tcaggcggtg tcgggagccg ggcatgctgc
      841 aatagaggcc cttgcggacg agtcagaagc ggtccttgca ggcttggatc cagtgccctc
      901 ggttctgcca tacaaatccg ccgaggtaca tcatcagata gccttcaatg tcattcctca
      961 cgtggactcg ttctgtgagg acggatatac ccgcgaggaa tacaagatga ccgatgagat
     1021 cagaaagata ctgcaagatc cctcgctcaa gatatctgcg acttgcgtcc gggtgccggt
     1081 cttccggtcc cattcggagt cgataaacct gcagacttgc caacgggctc cgatggatga
     1141 agtcagagct gtgttggctt cctctccggg catcaggttg atggatgatc ctgtgagttt
     1201 cgcatatcca atgcccatac atgtatccgg caaagatgat gtcgaggttg gcaggttgcg
     1261 cgaggatcca tccgcgccca atgccatgtg gctttgggtc gtaggtgatc agctcagaaa
     1321 aggcgccgca tccaacgctg ttcagatagg attggcgctc tatgatgacc ggaggtgacg
     1381 cgtgtgggca ttattgttca gaaatttgga ggcacgtcgg tggcggacgc ccaggcgtgt
     1441 gcgctggtgg tttcgaggat agcggaggcg aggcgcgcgg gcaatgacgt tgttgcagta
     1501 gtatctgcaa tggggcgaaa accggctcct tactccacag acgcccttct ggcgctggta
     1561 gattgcgctg gagatactct cccgcccagg gagcgtgacg ccatagctgc gtgtggagag
     1621 attatatcct ctgtggtgct tgtatcggct cttgttgcgt cgggctgccc tgccatgtcg
     1681 tttaccgggg cgcaggctgg aatcgagaca gacgacaatt tcggcgatgc tcgcatagtg
     1741 aggatcgacc ccgcgggcct ccacaatgcg attgcaagtg gtgtcgttcc tgttgtggca
     1801 ggattccagg gagtcacgga tgggggcgac gtaacaacgt tgggacgggg cggaagcgac
     1861 accaccgccg cagcgctggc agctgcgctt ggtgcggatc tggtcgagat atacacagat
     1921 gtagagggcg tgatgacggc cgacccacat atcgtgccaa acgccagatt ccaggagacc
     1981 atgacgtaca gcgagctgat agagatggcg aacttggggg cgaaggtggt ccacttacgg
     2041 gcggttgaga ttgcgagtca ggcgcggatt cctctggcga tcaagagcgt tttttcggat
     2101 tgcccgggaa ctttggtgac tgacattccg ccacaacgaa tgcctattgc ggtgccagac
     2161 cggtttgttt cctctgtgac gtgcataggc aacatggccc agatagtgct tgaggaatac
     2221 gatttcaaag ctgatgacgc tgcactcaac gtcttttcta ccatggcgga taatcatatc
     2281 tcagtggaca tgatcaacgt tttccccgac atgatcgcct tcatcgtctc tgcaagggat
     2341 tcggcgcgtg ctgtgcgtgc cctcgacagg ctgggccttt cggcagtggc taatcctggt
     2401 tgcgcaaagg tgtctgtagt aggcgagggc atgagaggag ttcccggggt catgtccagg
     2461 gtagtgaggg ctcttcacgg cgccggaata cgaattctgc agacctctga ctcccattac
     2521 tccatctcct gcctggttcc ggaggtcgat atggagcgtg cggcgcgcgc actccattcc
     2581 gaatttgagc tggagaaata agttcgcagt tggcgatctg ggagggaatt acattgaaga
     2641 agaacggaag acgcattcgc gtgatcacgg caatggcaac gcccttcgcc cctgatctgt
     2701 ctgtggatta cagaaaagcg cgagagctcg caagaaggtt aatcgagaac ggttcggacg
     2761 ggctcgtggt ctccgggacg accggcgaat ctccaacatt gacgcatgaa gagaaactcg
     2821 agcttttccg cgtagtcttg gaagaggtgg gcggtgaagc ggcggtaatt gcagggaccg
     2881 gggggaacga tactcgcacg agtatagagc tgacgcagga agcggagaag ttgggcgtgg
     2941 atggcgcaat gttggttacg ccctactata acaagccgcc gcaagcagga ttgtatcggc
     3001 actttgccag tgtagcccga tcgacttcac tgccgctcat gatatacaac gttccaggca
     3061 ggacctcagt taacctcagc gccgatacga tggccaagtt agccaatgat ttttcgaaca
     3121 tagtggcggt caaagaggcg tcgggcagcc tcgaacaggc agctgagata agatcgaaag
     3181 cgccaaaggg attcacaata tacagcgggg atgacaacat gaccataccc atgatgtctg
     3241 tgggcggcga tggggttgtg agcgtggcgt cgcacgtggc tggccctggg attcagcaga
     3301 tggttacagc gttcatcgac ggagatgtgg agcttgccgc agagatgcac cggcggttgt
     3361 tgccattatt caaggtgata ttcattacca ctaaccccat tccgatcaag gcagccttga
     3421 agcttgcagg atttgatgcg ggcggactaa gaccgccgct ggttgaagcg accgatgcag
     3481 aacttgggca aatagaagcg gtcatggagt cgcttgggct gatctaatac agtgcatatt
     3541 ggcagggtca tacgtggtga tggcctacca gacgtcaaca ggatccgcgt tatcttcact
     3601 ctgctttcag tcttggaatc agacaatcga gcgccctatg tccgtgcact gggcgctctt
     3661 tgggcttgct gtttcggcag gcaaatcacc tgtgcgaggt gttcgtttct ggcctcggct
     3721 cgtatgaagt tgatcattag atgaagcggg gtactggcgc ggagcgattc ctacgcctta
     3781 aagccgaaat cagtgtcttc atgcacggcc ggcaatcata cataaagtca acag
//
LOCUS       DAOSAI010000244         3831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15076_length_3831_cov_3.595074, whole genome shotgun sequence.
ACCESSION   DAOSAI010000244 DAOSAI010000000
VERSION     DAOSAI010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15076_length_3831_cov_3.595074"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLM83_08160"
     CDS             complement(<1..510)
                     /locus_tag="PLM83_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOK71413.1"
                     /translation="MAKPLVAIVGRPNVGKSALFNRIIRSRSSIVDDSPGITRDRVYS
                     TAEWSGRSFAIVDTGGIEADPADDISTQTRQHAEHAMKAADVIIFVVDARSGITSDDK
                     DVAQLLRIAGKPVILAANKVDHPAHMDYEFYALGLGDPMPVSAVHGIGIGNLLDAVVQ
                     ALPPQTDEPA"
     gene            complement(516..1868)
                     /locus_tag="PLM83_08165"
     CDS             complement(516..1868)
                     /locus_tag="PLM83_08165"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOK71414.1"
                     /translation="MTPIMQPRARITQVDEGSRADAAGICPGDVLVSVNGHPIRDIID
                     YEYHVSSFTTRLEIEKPDGSMLTAVFNNDYDPRLGLRFDELVFDGEKHCANKCVFCFV
                     DQLPRGMRDTLYFKDDDYRLSFLQGNFITLTNLRESDLQRIMDMRLSPLYISVHSTDD
                     VTRCRLLGRTHAFPIMPTLKRLAEARIDFHCQIVMVPGINDGTVLAASIRDLQNLWPS
                     CVSVGVVPVGLTSHRQGLPELEPVSAGIARQTLEIVDSAQNECMKLFGNHFVYAADEF
                     YCLASLPFPTADAYDEYPQIENGIGLARLFLDEFAQIALELPCSVPGRICATVVTGVL
                     GARVIRDACDSLNGIHGVKVRLVPVENKFFGESITVSGLVVGADIIRSLQEEASRGRL
                     GDFVVIPDVMLKSGERSLLDDVTVEGIASSVGVPVVVAPTTARGLVESILALHPPNAR
                     "
     gene            complement(1969..2883)
                     /locus_tag="PLM83_08170"
     CDS             complement(1969..2883)
                     /locus_tag="PLM83_08170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK71415.1"
                     /translation="MDKRKAGLVRLAIIVAAIVVAGIIWIVYTNSAHVAIVNGERISR
                     RDFIARLEEQYGEDTLAELINETLLHQAAKKAGVEVTPEEVDKEVALLKEQIGPSYDM
                     LIAQYGWTDEQIKDIIEMNLLVYKISTKDITIEEDALMAFFEENKSDYDEPEQVLASH
                     ILVATEEEAKEVKQELSEGADFAELAKEKSLDVATAVNGGDVGWFPRGRMTEEFEKTA
                     FALSPGEVSGPVKTEFGYHIIKVMDRKAAREASFDEVRDDIERNLKMQQSKTAQQLVS
                     ELKLDSDITITDSKYKDLGTTTPFGIKE"
     gene            complement(3053..>3831)
                     /locus_tag="PLM83_08175"
     CDS             complement(3053..>3831)
                     /locus_tag="PLM83_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOK71416.1"
                     /translation="VGRRFVEDLNVYVGQTLRLKVREVERLRSNVVLSHRAVLEEEER
                     LAKERAFQTLERGQVLDGTVTRLVSFGAFVDIGDGVEGLLHISDISWSRIKHPGEVLA
                     EGQSIKVLVLNVDRERERISLGYKQLLPDPWENIGQRFPVGSVVSGTVTKIVDFGAFV
                     RLEDGIEGLVHISQLADRRVGRPDEVVSVGETVTVKVIGLREQEHRISLSIRQAMEDR
                     ERNEYRRYMKESKESDVVTIADRINYDPSKLKGSTDGDDR"
BASE COUNT          874 a         1106 c          919 g          932 t
ORIGIN      
        1 agcaggctca tctgtttgag gaggcaatgc ctgtacaact gcgtcgagta ggtttcctat
       61 gcctattcca tgaaccgccg agacgggcat cggatcccct agcccgagag cgtagaattc
      121 gtagtccatg tgggccggat gatccacttt attcgcggcg agaatcacag gttttccggc
      181 tatgcgcagc agctgggcca cgtccttatc gtcagatgtg atgccagaac gggcatctac
      241 aacgaaaatg atgacatcag ccgctttcat ggcgtgctcg gcatgttgtc ttgtctgcgt
      301 cgagatgtcg tctgctggat cggcctcaat cccacccgta tcgacaatgg caaaggatct
      361 acccgaccac tctgccgtgg agtacaccct gtcacgggta atgccgggcg agtcgtccac
      421 gatcgacgat ctgctcctta ttatgcgatt aaagagcgca gacttgccga cgttaggccg
      481 tccaacaatg gctacgagcg gtttagccac atcattcacc tcgcattcgg tgggtgcaga
      541 gcaagtattg actcgacgag ccctcgcgcg gtggtagggg ctaccaccac cggaacgccc
      601 acagaagaag ctatgccctc cactgtgaca tcgtcaagca gagacctttc gccgcttttc
      661 agcatcacgt cggggatcac gacgaaatcg ccaagacgac cccgggatgc ttcttcctgc
      721 aaagaccgta tgatgtctgc gccaaccacc agtccggaaa cggtaatgga ttctccgaag
      781 aacttattct ccacaggcac caaccggacc tttaccccgt gaatcccgtt caaagagtcg
      841 cacgcgtcgc gtattactcg cgcgcctaga acgcccgtta ccacagttgc gcagatcctt
      901 cccgggactg agcatggaag ttctagagcg atctgagcaa attcatccag aaataggcgt
      961 gcaagtccta tgccgttttc aatctgagga tactcgtcat atgcgtcagc tgtaggaaaa
     1021 ggcagagacg caagacagta aaactcatct gctgcgtaaa cgaagtggtt tccaaaaagc
     1081 ttcatgcatt cgttctgcgc cgaatcgact atctccagag tctgacgggc gataccagct
     1141 gatacaggct caagttcggg cagaccctgg cggtgggagg taaggcccac aggaactacg
     1201 cccaccgaca cacatgatgg ccacaggttc tgcagatccc tgatgctcgc ggcgagcaca
     1261 gtgccatcat ttatacccgg aaccatcact atctggcagt ggaaatcaat ccgtgcctct
     1321 gccagccgct tcagcgtagg catgatgggg aaggcatgtg tcctcccaag caacctgcac
     1381 ctggtcacat cgtccgtgct atgcacggat atgtacagtg gagaaagccg catgtccatt
     1441 atgcgctgaa gatcgctctc cctcaagtta gttagggtga tgaagttccc ctgaagaaaa
     1501 gagaggcgat aatcgtcgtc cttaaagtat agagtatccc gcatgcccct gggcaattgg
     1561 tctacaaagc agaaaacgca tttgttcgcg cagtgcttct ccccatcgaa cacgagctcg
     1621 tcgaatcgaa gcccaagacg gggatcgtaa tcattgttaa agactgctgt cagcattgac
     1681 ccgtcaggct tttcgatctc gagcctcgtc gtgaaggagc taacatgata ctcatagtct
     1741 attatgtctc ggattggatg tccatttaca gacaccaata cgtctccggg gcaaatgccc
     1801 gccgcatcgg cacggctgcc ctcatcaact tgtgtaattc tagcccgagg ctgcatgata
     1861 ggcgtcatag acccgtgcct ccaagaaaat caaaagggag aggaaagtcg cactctcctc
     1921 tcctcgtagg tgttatccgt ctgccgctct tgctagagct agcaaaggtt attccttgat
     1981 gccgaatggg gtcgttgtcc caagatcctt gtacttggaa tcggttatcg taatatcgct
     2041 atcaagcttc agctcgctca ccagctgctg agcggtctta gactgctgca tcttaaggtt
     2101 tctttcgata tcgtccctaa cttcgtcgaa agaggcttcg cgagcggctt ttctatccat
     2161 gacttttatt atgtgatagc cgaactctgt cttgactggt ccgctgacct cgcccggcga
     2221 aagagcaaat gcagttttct caaattcttc tgtcattcgt ccgcggggga accaacccac
     2281 atcgccccca ttgactgcag ttgcaacatc gagcgacttt tccttggcaa gctcggcaaa
     2341 gtctgcgccc tcagaaagct cctgcttgac ctctttggcc tcttcttcag ttgcgactag
     2401 tatatggctg gccagaactt gttctggctc gtcataatct gacttgttct cttcaaagaa
     2461 ggccatcaga gcatcttcct ctattgtgat atccttcgtg gatatcttgt atacaaggag
     2521 gttcatctcg ataatatctt tgatttgctc atcggtccag ccatactgag cgatcagcat
     2581 gtcgtaagaa ggcccgatct gttccttgag tagagctact tctttatcta cctcttcagg
     2641 cgttacttcg acccctgcct tcttggcggc ctggtgaagc agagtctcat ttatcagttc
     2701 tgctagggta tcctccccgt attgctcctc aagcctcgcg atgaaatcgc gccgcgatat
     2761 cctctcgccg ttaactatgg ccacatgtgc cgagttggtg taaactatcc atattatccc
     2821 ggccacgacg attgcggcca caattatggc caaccgaaca agaccggcct tgcgcttgtc
     2881 catgcttgcc aacccccttc tctcaactac gatcctgctt acgtcctgct actggcttaa
     2941 acgaactatg ccaggcacga gttccacata aaaatgctac ggctagtcta gcatagtgac
     3001 gcaggactgt caacgaaacg ccagcataca ggctgatatg atggcagcaa tcctacctgt
     3061 cgtctccgtc agtcgaaccc ttaagcttgc tgggatcgta gttgattcgg tcggcgatgg
     3121 tgacgacatc gctctcttta ctttccttca tgtacctgcg gtattcattg cgctccctat
     3181 cttccatcgc ttgccgtatt gacaggctga tgcgatgctc ctgctccctg aggcctatga
     3241 ccttgactgt cacagtttcg ccaactgaca cgacctcatc tggccttcca acgcgtctgt
     3301 cggcgagctg cgagatgtga acgagtccct caatcccgtc ttcaagccgg acgaaggcgc
     3361 caaaatcgac gatcttcgtc acggttccag agactacaga acccacaggg aaccgctggc
     3421 caatgttttc ccacgggtca ggaaggagct gcttgtatcc gagggatatc cgttcgcgct
     3481 cgcgatctac attgagcacc aggaccttga tggactgtcc ctcagcgagc acttcgccag
     3541 gatgctttat cctggaccaa gagatatcgg agatgtgaag caggccttca actccgtctc
     3601 caatatccac aaatgctccg aacgacacga gtcgggttac tgttccatca agcacctgac
     3661 cccgctcgag cgtttggaac gcgcgctcct tagcaagtct ttcttcctcc tcaagcactg
     3721 cacggtgaga aagcaccaca ttggaacgga gcctttcaac ttcgcggacc ttcagcctca
     3781 acgtctgtcc aacatagaca ttcaaatcct ctacgaacct gcgccctacc t
//
LOCUS       DAOSAI010000245         3827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15104_length_3827_cov_3.025186, whole genome shotgun sequence.
ACCESSION   DAOSAI010000245 DAOSAI010000000
VERSION     DAOSAI010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15104_length_3827_cov_3.025186"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(789..2015)
                     /locus_tag="PLM83_08180"
     CDS             complement(789..2015)
                     /locus_tag="PLM83_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOK71417.1"
                     /translation="MPTTRLYYDDPYLSDFQCNILRCEPSTRNSSPTPLYEAVTDVTA
                     FYPNSGGQPSDRGILAGADVVDVVERDGEIIHLTVGSVPLGLADGRVDIERRFDHMQQ
                     HTGQHILSAAFANLFGLDTVGFHLGDETATIDLPICEISNDQAAAAEMLACEVVFQNR
                     PVTTTFRKPCDVDESYLRKLPNNAESIRLVEIDGFDINACCGTHVRATGEVGLIKIIG
                     IERIRSVTRVEFVCGRRALADYRWKNDVVHLAASQLSIHGKELPEAMLRLQNQIRQGR
                     RGLEEALDRLRDYEAEEIYSAAVPLNPSGVRVIALMSQRQDIESVRAVAQKISCKPKV
                     AAFLGCVNGESANLVFARSNDIPLDMSELLKQALPAIDGKGGGSPAYAQGGGKNPDGI
                     PEALALVAGLAKDALG"
     gene            2118..2795
                     /locus_tag="PLM83_08185"
     CDS             2118..2795
                     /locus_tag="PLM83_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOK71418.1"
                     /translation="MAREYLALRDEGRAELIVKKSRFIATARPVDSEDAARDFIDSIR
                     KEFWDATHNVYAFTVGENDEVVRSSDDGEPAGTAGRPVLEVIRREKIKYCAVVVTRYF
                     GGTLLGAGGLVRAYSSAARDGLHAAGIVRVRLLQKVRFVIDYVALGIVQNYLAEAGYE
                     MDDVEYADRVAMTVMVPSSELDLFEARMMDLLAGDFNPEVGEEVVGFADVKEGEGLGG
                     EDGTGEE"
     gene            complement(3190..>3827)
                     /locus_tag="PLM83_08190"
     CDS             complement(3190..>3827)
                     /locus_tag="PLM83_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOK71419.1"
                     /translation="RVQPAQLQAAVLPHARRGFFAVELEDAKQAVEKLPWVERAEVRK
                     AIPSPYHSSDVSDAFRLPAAPQAYSGYSVVNLPDFSDWEKFKHETSVLGFCASRHAME
                     FFRPALMREGVWDSRKISSADQGQIVRVAGLVVRPHRPPTKSGKTVVFLSLEDEYGLT
                     DVTVFDDVYQRQGAAIFKSPALIVTGTISRRGNGISVIAKSIESLGEKCNR"
BASE COUNT          750 a          961 c         1182 g          934 t
ORIGIN      
        1 gttgtgaccg aagagatcat gctggcaaga tccaggatgg tgaaggcccg ttccattgcg
       61 tacttgacag gcaggccttg ccactacgtc ttgctcaagc tctcaccctt tgcctacgtg
      121 cgcgccagtg cggcttttta agagcttatt attcctacga gttggtggag cccaggtcgg
      181 gtgggtcggc gttatggctg tgctcacttt cattctttac ggcttggggg tgaagcggaa
      241 caatgtcaat agaggctagg actgctccgc gaaaggcgag ggggagtggt tgggacggct
      301 agtctcatga ggcggcaagt cttgcagcgg cggctgttta cgccagcttt gcaactttcg
      361 ctggcagcct cgcttttttt tatatgaaat gcccagactg tcagcgtcca gatgccccag
      421 gcaatgatca ccttctccgg ataccctggg tcactgtttg aagtcgctgt ccggacattg
      481 tgataccagt aggctttttg catatgtccc cgttgagctt ctcggcaatt tgagttggcc
      541 gcttcttgcg ggactgattg cctttgcagc gggcagcgct gtagcagcaa aggtattgtt
      601 tcaaacagga ctgcgccgct acgagtcagg caatctgatg tcgattaggt catgacttcc
      661 gttgggggta ctgttgcctc gtttgcctgt gcgcaagtcg gccgcgcctt ttgaatggac
      721 gtcatgacct gccagtatca ttttgctcgg gctatggcta cctatcgact tggttgaatc
      781 acagggcctt atcccaaggc gtctttcgcc aggcctgcaa ccaaggcgag ggcttcggga
      841 attccgtcag ggtttttccc gccgccctga gcataagcag gtgagccgcc acctttgcca
      901 tctatggcag ggagtgcttg tttgagaagc tccgacatgt cgagggggat gtcgtttgac
      961 ctagcgaaga ccaaattggc tgactcccca ttgacacagc ccaagaaggc ggcgactttc
     1021 ggcttgcagg agatcttctg cgctaccgcc cggacggatt caatgtcctg tctctggctc
     1081 atgagcgcga tcactctcac gccagatggg ttcagaggca cggcggcgga gtatatctct
     1141 tccgcttcat agtcgcggag cctatctagg gcctcttcta gacctcgtcg cccttggcgg
     1201 atctggtttt ggaggcggag catcgcctct ggcagttctt ttccgtgaat tgaaagctga
     1261 gatgcggcga ggtgcaccac atcatttttc cagcggtagt cggccagggc tctccggccg
     1321 cagacgaact cgacacgggt aacgcttcga atgcgttcga tgccgattat ctttatcaga
     1381 ccgacctcgc cggtggcccg gacgtgcgtg ccgcaacacg cattgatatc aaaaccgtcg
     1441 atctcgacga gtcgaatgct ctcggcgtta ttgggaagtt tgcggaggta tgattcatct
     1501 acatcgcagg gctttcggaa cgtggtcgtg actggcctgt tttggaacac cacctcgcat
     1561 gcgagcattt cagctgcagc tgcctgatca ttgctgattt cgcatatggg aaggtctata
     1621 gttgcggtct cgtcgccgag gtgaaatccg acggtatcga ggccaaacag gttggcgaat
     1681 gctgccgaga gaatgtgctg gcctgtatgt tgctgcatgt gatcgaacct gcgctcgatg
     1741 tccactcgcc cgtctgcgag gccgagtggt actgatccaa ctgtgaggtg aattatctct
     1801 ccgtcccgct ccactacatc gactacgtcg gcgccggcca ggatcccgcg gtctgagggt
     1861 tgtccgccgg agttggggta gaatgcggtg acgtcggtga ctgcctcata cagaggggtg
     1921 ggggaggaat tgcgagttga gggctcgcag cggaggatat tgcattggaa atcgctaaga
     1981 taaggatcat catagtataa ccttgttgta ggcacctgaa gaattcgcct cctcaagctc
     2041 tgatgagctc cggttgcaga tgattacttc tactgccgag ctcgattccc ttttatctgg
     2101 tcgaaggagt ggtgatgatg gcacgtgagt atcttgccct tcgggatgag ggccgagcgg
     2161 agctaatagt aaaaaaaagc aggttcatcg ctacggcacg tcctgtggac agcgaggacg
     2221 cggcccgaga ctttatagat tcgatccgta aggagttttg ggatgccact cacaatgtat
     2281 acgcctttac tgtcggagag aacgacgagg tggttcggtc gagcgatgat ggtgagcctg
     2341 ccggaacggc cgggaggccc gtgttggagg tcatccggcg agagaagata aagtactgcg
     2401 cagtcgttgt aacccgctat ttcggcggca cgcttctcgg ggcgggcggg cttgtgaggg
     2461 cttattcgtc cgccgctagg gatgggttgc atgcagccgg catcgtaagg gtcaggcttt
     2521 tacagaaggt tcggttcgtc atagactacg tggctttggg catagtccag aattacttgg
     2581 cggaagccgg gtatgagatg gatgatgtgg aatatgcgga tcgggttgcg atgacggtta
     2641 tggttccgtc gtcggaactg gatctgtttg aagctaggat gatggacttg cttgcgggcg
     2701 acttcaaccc tgaggtgggg gaggaggtcg tagggtttgc tgatgtgaag gagggagagg
     2761 gtttgggcgg ggaggacggg acgggagaag agtagtagag taatgtggat cgggatgagg
     2821 gtgaggatgg ggacggtagc gccgttttgg ccacgccgca ggcctaccgg gaaaagctgc
     2881 aagatatgtg gctgagcagc tgtgcatggg gttggacgca gctgcatgct cgtttctctt
     2941 cacgttctta gtgcggaatg cccaatatgg gttattcgat catcctcaaa ttctcgccct
     3001 tagcattggg ctgtggcctc aggcggcgga agtagctcag ggctttccat tacgctgctt
     3061 cgttttcatg cagcatgcgt cgaatgtcgt gataagctct tgcgcgatcc tcgagtccgt
     3121 attacctaca gttcagactt tgtccgccta atctataaga cgcttctcgc aagttactca
     3181 gcttgcctac tagcggttgc atttctcccc gagactttct atggacttag ctatgactga
     3241 aatcccattg ccccggcgcg agatcgttcc cgtgacgatt agtgcagggc ttttgaatat
     3301 tgcggcgcct tgcctctggt atacatcatc aaagactgtg acatctgtga gcccgtattc
     3361 atcttcgagc gagaggaaga caacggtttt gccgcttttc gtaggtggcc tgtgcgggcg
     3421 gactacaagc cctgccacgc gcacgatttg cccttgatct gctgatgaga tcttgcgcga
     3481 atcccaaacc ccttcgcgca tgagcgcagg gcggaagaac tccatggcat ggcgagatgc
     3541 gcaaaagccg agtaccgatg tttcatgctt gaacttctcc caatcggaga aatcagggag
     3601 attgacaact gaatagcctg agtaggcctg aggggcggca ggtagccgga aggcatcaga
     3661 gacgtcggaa gaatggtatg ggctggggat cgccttgcgc acttcggcgc gctccaccca
     3721 gggcagcttc tcgaccgcct gcttggcgtc ctccagctcc accgcgaaga agccgcgacg
     3781 cgcatgcggc agcaccgctg cctggagctg cgccggctgc acgcgcg
//
LOCUS       DAOSAI010000246         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15111_length_3826_cov_2.985150, whole genome shotgun sequence.
ACCESSION   DAOSAI010000246 DAOSAI010000000
VERSION     DAOSAI010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15111_length_3826_cov_2.985150"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            944..2056
                     /locus_tag="PLM83_08195"
     CDS             944..2056
                     /locus_tag="PLM83_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOK71420.1"
                     /translation="MPPKVYFVSAKDDHGVSLPDKMSRLLDAADIGKGISKGDLVAIK
                     LHVGEPGNLSFVPPQLVRRVVDKVRAVGGNPFLTDANTLYVGQRSNGVNHAMAAISNG
                     FGVEVTGAPFIVADGLTGQDYVGVSMPGPFIREAKIASAIARADAVISVAHFKGHQLS
                     GFGGTLKNLGMGSASRAGKQVQHSDLKPRTNQERCVGCGRCVRYCPAEAIALVDRRAE
                     ADWGKCIGCGECVVVCRHKAMQINWDEGAPGSMQRRMAEYAAAALAGKEGKTGFVNVA
                     IRISPNCDCNPVNDIPVVSDIGLLASHDPVALDQASSDMVVRAAGAPGGACCDVGEHE
                     DKFKSVFPNTDWNTQLEHAEAIGLGSREYELVELMR"
     gene            complement(2312..3127)
                     /locus_tag="PLM83_08200"
     CDS             complement(2312..3127)
                     /locus_tag="PLM83_08200"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK71421.1"
                     /translation="MVSETLALTWYDAIFDRHSTRNYDGRPVPDGLLDALAPLLSGFA
                     PLCEGARAVIVADSASEVFTGLVGPVGKITAPPVALAFIADTSHPHYQVATGYLGEGI
                     VLEATSMGLATCWVSGSFRRNEAERRVTLAASEKVMALTPLGFPASSETFGAKAIRTA
                     ASAHKRKPLSSIVSGLPQKDWPPGCGEALEAARLAPSALNRQPWQFNVGEERVTISTS
                     GLDPFPIAPVKLDCGIAMLHFEIAAKANGLRGEWRMSDKAPQVAEFVFTHSSL"
     gene            3340..>3826
                     /locus_tag="PLM83_08205"
     CDS             3340..>3826
                     /locus_tag="PLM83_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71422.1"
                     /translation="MGVFGWHDKRSSAEKEALQHACTYLYGASQLISYGIALDDNIEV
                     VAEQFPHFGRDNLEKVRESLLKHAEASLRSARLYLECHPDLADFEPNWTPEDTGLIVE
                     FLQCEYGDEQPELSDAMRGDLLAPVYVFLERCVNERRKSSSAPAADIEDADRTCDGEA
                     KE"
BASE COUNT          873 a         1017 c         1144 g          792 t
ORIGIN      
        1 gccgtccggc gtgagcgtta tcgcgaccct atctccaggc acgatcccgc ctgcttgtgc
       61 cagcggagat accgccttgg accgtgccgt gcagaagacg ctgccacatg atgtctctac
      121 ttcaaagaaa gaagatgctt gaagaacaac gccctgcaat agatcactgt tcaattcgtc
      181 cgatgcctcc tgtgcgtgtg gtggatcgtc gacgtgccca acgtcccgtt gtcgccatgg
      241 aggaaacgga cgcaatccgg cttgttccgc agcagcctga agcggcagaa caacaaagcg
      301 cgcccgcttc cgcaggcgcg catatcacgc tcgtgtagaa tcgatcatca ggacagggca
      361 aagcctctac gcatggaccg cagatggcat acagcctgca tgtgaacgaa tgctttgcgg
      421 gccaagccga gcttgagcag acaagcgctt gcccatgccg tccaacggca actacctgtt
      481 cagatcggcc tgtgcgacaa ccggcgaacg gtgggttgga caatattcgt gcgaagaacg
      541 ctcaatcatc atgtaggaca taaaaaccac ctcttccgcc gggccggatc taccgaaccg
      601 taccgctcat atctgcacca tatgctatca tttggccgtt tcgatgtcaa acactgcaca
      661 ggttgggttg tgccggagaa tttggggcgt gcgagggacg acggctcgtg gggttagact
      721 ccgttttccc agcctatctc cgacaggaag caccggccct tgctgccatt cataccaagc
      781 acgcaaacgg ctcgacagtg cctgcacatg atagcagggc ttctggtgct cagccttggg
      841 cagcaatcgg cgtgcgcctg gatgcggggt ggatggaggt gaatggcatg tgcatgtcaa
      901 aatagcatca gcaagcaacg cgtgttagcg aggaggagac ttgatgccac cgaaggtgta
      961 cttcgtcagt gcaaaggatg accacggcgt gagtttgccc gacaagatgt cgcggctgct
     1021 cgacgccgcc gatataggca aaggtatatc caagggcgac cttgttgcaa ttaagctgca
     1081 tgtaggggag cccggaaacc tatcattcgt gccgccgcag ctagtgcgga gggtagtcga
     1141 taaagtgaga gcagtgggag gcaacccgtt tctaacagat gccaatactc tctacgtagg
     1201 tcaacgctcg aacggagtga accatgccat ggctgcaata tccaacggct ttggcgtgga
     1261 ggttacgggc gcaccgttca tcgtggccga tggcctcact gggcaagact atgttggcgt
     1321 ttccatgccg ggccccttca tacgtgaggc aaagatcgcg tccgcaatag cccgcgcaga
     1381 tgccgtaata tccgttgccc atttcaaagg gcaccagctg tcagggtttg gcggaacact
     1441 gaagaatctt ggcatgggga gcgccagcag ggcgggcaaa caggttcaac attccgactt
     1501 gaagcccagg acgaatcagg aaaggtgcgt cgggtgcgga cgatgtgtgc gatattgtcc
     1561 tgccgaggca atagcactcg ttgacaggcg tgctgaggca gactggggaa agtgcatcgg
     1621 ctgcggagag tgcgtggttg tgtgcagaca caaggctatg cagataaact gggatgaagg
     1681 ggcgcccgga agcatgcagc gcaggatggc cgaatacgcc gcggccgcgc tcgccggcaa
     1741 ggaagggaag acgggttttg tgaacgtcgc aatcaggatc agtcctaact gtgactgcaa
     1801 cccggtcaat gacattccag tagtctcgga cataggattg ctcgcatccc atgaccctgt
     1861 tgcactcgat caagccagtt cggatatggt ggttcgggca gccggagccc cgggcggggc
     1921 atgctgtgac gtgggcgaac atgaagacaa gttcaaatcc gtctttccca ataccgactg
     1981 gaacacccag ctggagcacg ctgaggctat aggccttgga agcagagaat acgaacttgt
     2041 tgaactaatg cgttagcaag ggtcctgccg aggcagacat gcggctggtg atggcgcccg
     2101 gccaacgggc attgaggctc gcagctattg cagtggccta cgtggcattc gtcttcacgc
     2161 cagaaaagct ttgatatggg cctgcctgtt atatgaataa cgaagggaga tctgtgggag
     2221 gcgggcctga gagcgtgtgc atcccggctg aagccttcag gccatcaaaa cacctcccac
     2281 aaaaccctcg gttctcgtat gtgtatggga tctacagact ggagtgtgtg aaaacgaact
     2341 ctgccacctg gggcgcttta tcggacatcc tccattctcc gcggagaccg ttggccttcg
     2401 ccgctatttc gaaatggagc attgcgatcc cgcagtccag cttgaccggg gcgataggaa
     2461 acggatcaag tccggacgtg gatatcgtca cccgctcttc gcctacattg aactgccatg
     2521 gttgcctgtt aagcgctgac ggcgcaagcc tggccgcctc aagagcttca ccgcagccgg
     2581 gtggccagtc tttctgaggc agtccgctga caatcgatga caggggcttt ctcttatgcg
     2641 cagaagccgc ggtccttatt gccttcgcgc cgaaagtctc gctagacgcg ggaaagccca
     2701 gtggcgtcag ggccatgact ttttctgatg ctgcaagggt taccctgcgt tctgcttcgt
     2761 tgcgcctaaa actcccgctg acccagcagg tagcaaggcc cattgaagta gcctcaagga
     2821 cgattccttc acccaagtag cccgtggcca cttgataatg gggatgggag gtgtctgcga
     2881 tgaacgccag cgcgacgggg ggagctgtga ttttgcccac agggccgaca aggcctgtga
     2941 aaacttcaga tgcgctgtct gcgacgataa cagcgcgggc cccttcacat agcggtgcga
     3001 aaccggaaag aaggggcgca agcgcgtcaa gcaggccgtc gggaacgggc ctgccgtcgt
     3061 agttcctagt cgaatggcga tcgaatatcg cgtcatacca tgtcaatgcc agagtttcag
     3121 aaaccattcc cttcctcctt tgctttccac actaacgttc gtctgcgtcc ttgttcaact
     3181 tgaggctaag cgattcctgc aaccgactgt gtaagaatat gtgctggacg tgcgtggcag
     3241 aagctgtatg cactaccgag gccatacagc cgtttgaagg gaaacgcgct ttgatggcta
     3301 agcaagaaag gtatagtctt atgacagggg ggagcaagga tgggcgtatt tggatggcat
     3361 gataagcgtt cgtctgcgga aaaagaggcg ttgcagcatg cctgcactta tctgtacggg
     3421 gcaagccagt tgatttctta cggaattgcg ctggacgaca acattgaggt agtcgccgaa
     3481 caattcccgc actttggccg cgataacttg gagaaggtgc gtgagtctct gttgaaacat
     3541 gctgaagcca gtctgagatc ggctcgactg tacctcgaat gccatccgga tcttgcggat
     3601 ttcgagccta attggactcc ggaagatacc ggacttatag tggagttcct tcaatgcgag
     3661 tacggggacg agcaacctga actatccgat gcaatgcgcg gcgatctgct ggctccagtc
     3721 tatgtttttc ttgaacggtg cgtaaacgag cgccgaaaga gcagcagtgc tcctgcagcc
     3781 gacatcgagg atgccgaccg gacctgcgat ggagaagcaa aagagc
//
LOCUS       DAOSAI010000247         3804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15227_length_3804_cov_3.612430, whole genome shotgun sequence.
ACCESSION   DAOSAI010000247 DAOSAI010000000
VERSION     DAOSAI010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15227_length_3804_cov_3.612430"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLM83_08210"
     CDS             complement(<1..334)
                     /locus_tag="PLM83_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HOK71423.1"
                     /translation="MTCAPIIREAKHAGFCRGVERAVRIACEAGQEALRSGSGGAVTL
                     GPLVHNPDVVSSLLKCGVEQVDSIESASGRVLIIPSHGLPPETIQAARERGLTLVDAT
                     CPHVLRSQR"
     gene            complement(331..978)
                     /gene="fsa"
                     /locus_tag="PLM83_08215"
     CDS             complement(331..978)
                     /gene="fsa"
                     /locus_tag="PLM83_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOK71424.1"
                     /translation="MKLFIDSANIDEIREVAEMGVVCGVTTNPSLVAKEGRDFKEVVS
                     EICRIVDGPVSAEVISQDADGMIREAAEISTWSPNIVIKIPMTSEGLKAVKVLAARGI
                     NTNVTLIFSSSQALLAARAGATYVSPFVGRLDDISSDGMNVVAETASIFDIHGIATEI
                     IAASIRHPMHVIEAALVGSDIATVPYKVLMQMAKHPLTDAGIERFLADWEQAIKK"
     gene            complement(999..1610)
                     /locus_tag="PLM83_08220"
     CDS             complement(999..1610)
                     /locus_tag="PLM83_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOK71425.1"
                     /translation="MFYSFAKFVMTLYLSIAFGFKVTGKENVPATGPVIIVANHASIL
                     DPIALGCACDRRIRFMAKAELFENPISAAVLRWLYAFPVRRGRVDREAYRQSMDILKA
                     GGALGMFPEGTRSETGELQKAYPGAARFAIQTGTPVLPAAVVGTFAGHRKGLKRFKEP
                     MVRVVFGELIWPKVDRAGATVREETDALAEEIMKRIGELIMIA"
     gene            complement(1583..2284)
                     /gene="cmk"
                     /locus_tag="PLM83_08225"
     CDS             complement(1583..2284)
                     /gene="cmk"
                     /locus_tag="PLM83_08225"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOK71426.1"
                     /translation="MSLEHSIAIDGPAGAGKSTVARMLAHALGYRYVSTGDLYRALAL
                     EVIRRGLDPYDEAAVVDVAKSVCIRVERSECGGQRTILNNEDVTEEIRSAEVDSVVSP
                     VSVYSQVRKHMVEAQRAIAYDADVVMDGRDIGTVVLAESAHKFYLTASAEERARRRQL
                     DYARQGRHVDLADLVEEIEKRDTIDSTRQISPLRPAPDAVVIDCTTLSPDEVVDTMLA
                     ALRRPASCFTLSQNS"
     gene            complement(2383..2931)
                     /locus_tag="PLM83_08230"
     CDS             complement(2383..2931)
                     /locus_tag="PLM83_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71427.1"
                     /translation="MQNWDVATIVFSALTGLALSACILVQVLLSCGVIPRSVLMIERI
                     EGHPSWSWTPSPGKSGDSGKPDVRAGAAVEAGGILLKLSALDNGLECAEPTLTVLVNG
                     KPVADFKYGSAAVVIHRGDLLEVEVNDADEDRIDSTAGGAHVEQLEIEVEDVTPNVTK
                     PQKGDKCSIGPGRTQICNVIII"
     gene            complement(2936..>3804)
                     /locus_tag="PLM83_08235"
     CDS             complement(2936..>3804)
                     /locus_tag="PLM83_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOK71428.1"
                     /translation="GLAVADGKVKGVIDSGGNNSWADAVIIATGGISYPGTGSTGDGY
                     RIAAEAGHTIVQPLPALVPMETVEDWPGSVAGLSLRNVELSLYDRGRRIGREFGEMLF
                     THFGVSGPIVLSLSRAVCRSLRESEAIESGRFTLSIDLKPALDEATLHRRIQRDLVAA
                     SKKQLKNSLGMLLPHSIIPVVISLAQVDETIPANQITTAQRSALVETLKRMPLTVLKT
                     RPIEEAIVTSGGIALREVDPRTLESKLLPGLYFCGEVLDIDAPTGGYNLQAAFSTGRL
                     AGMSAAMSRRSG"
BASE COUNT          825 a         1105 c          941 g          933 t
ORIGIN      
        1 cccgctggga tcgaagcaca tgcggacatg tggcgtccac gagcgttaac cctctctccc
       61 gggcagcctg aatagtctcg ggagggaggc catgcgacgg aattatgagc acacgacctg
      121 aggcagactc gatggagtca acttgttcga cgccacattt aaggagactc gacaccacat
      181 cggggttatg gaccaaaggc cccagggtta ccgcgccgcc tgagccggag cgcagtgctt
      241 cttgccctgc ctcgcacgcg atgcggacag ctcgttcaac cccgcggcaa aatccggcgt
      301 gtttcgcctc tcttatgatg ggcgcacaag tcactttttt atcgcctgct cccagtctgc
      361 taggaatcgc tctatccctg catccgtaag ggggtgcttc gccatctgca tcaatacctt
      421 gtaagggaca gtagcgatat cagatccgac caacgcagct tctatgacat gcattggatg
      481 gcggatgctg gcggctatga tctcggttgc aatgccgtga atatcgaaaa tagaagccgt
      541 ttccgcgacc acattcatcc catcggagct aatgtcgtcg agacgtccca caaacgggct
      601 cacgtatgtg gcgccagctc tggcagccaa caatgcttgg ctcgatgaga atatgagtgt
      661 aacgttggtg ttgattcccc tggcagcaag aacctttacg gctttgagcc cctcagatgt
      721 catcggtatc ttgatcacta tattggggga ccaggtcgag atctcggctg cttcgcggat
      781 cataccatcc gcgtcctggc tgatcacttc ggcgctcact ggcccgtcta cgatcctaca
      841 gatttcagag acaacttcct tgaagtcgcg gccttctttt gcaacaagcg acgggttggt
      901 ggttacaccg catacaacac ccatttcggc aacttcgcgt atctcgtcaa tatttgcgct
      961 gtcgataaaa agcttcacct tcgcacactc ccatattctc acgcaatcat aatcagttca
     1021 ccgatgcgtt tcattatttc ttcggcgagc gcgtccgtct cttccctgac agttgctccc
     1081 gctcgatcga cttttggcca tatgagctca ccaaagacca ccctgaccat gggttctttg
     1141 aacctcttca accctttgcg atgccccgca aaggttccaa caaccgctgc aggaagcacg
     1201 ggtgtccctg tctgtatggc aaaacgcgca gctccaggat acgccttctg cagttccccg
     1261 gtttcgctgc gcgtgccttc aggaaacatg cctagagctc ctccagcctt aagtatatcc
     1321 attgattggc gatatgcttc acggtcaacc ctaccgcgcc tcacaggaaa agcataaagc
     1381 cacctcaaaa cggcagccga gatgggattc tcaaaaagct cggcctttgc catgaagcgt
     1441 atccggcggt cgcaagcaca gcccagagct atcgggtcca ggatgcttgc gtggttcgct
     1501 actatgatta ccggaccagt tgcgggcacg ttttcttttc ccgtcacctt aaatccgaag
     1561 gcaatggata agtatagtgt catcacgaat tttgcgaaag agtaaaacaa gaagcaggcc
     1621 tcctaagagc agctaacatc gtgtcgacca cctcatccgg agacagtgta gtgcagtcga
     1681 tgacaactgc gtcaggcgcc ggtctgagcg gagagatctg tcttgtacta tcaatggtgt
     1741 cgcgcttctc gatctcctcg actagatccg ctaggtcaac atggcgtccc tgtctggcgt
     1801 agtcaagctg tcgccgtcga gcccgctcct cagctgatgc cgtcaggtaa aacttatgcg
     1861 ccgattctgc aagcacaact gtacctatgt cacgaccgtc cattactacg tccgcgtcgt
     1921 atgcgattgc tctttgggct tcaaccatgt gcttcctaac ctgtgagtac actgacaccg
     1981 gtgagacaac tgagtcaact tctgcgctgc gtatctcttc ggtgacgtcc tcattgttga
     2041 gaatagtccg ctgtccgccg cactcgctgc gctcaaccct tatgcaaaca gattttgcga
     2101 catccacaac cgccgcctca tcgtagggat ctagccctcg acgaatgact tccagggcca
     2161 aagcgcggta gaggtcacca gtgctcacat acctatatcc aagtgcatgg gcgagcatcc
     2221 gcgcaaccgt gcttttcccg gctccagccg ggccgtctat ggcgattgaa tgttccagcg
     2281 acatacatca acgctctcca atcagtgtac aatttaaatt tattctacgc tagtacgctt
     2341 attcctctgc gacaacgaaa aatccttctg cgttcgtata atctatatta ttataacatt
     2401 acatatttga gtgcgtccgg gaccaataga gcacttatct cccttctgtg gctttgtcac
     2461 gtttggagta acatcttcca cctctatttc tagttgttcc acgtgcgccc caccagccgt
     2521 cgagtcgatc cgatcttcat cggcatcgtt tacctccacc tccagcaagt ctcccctgtg
     2581 aattactact gccgcgcttc catacttgaa atctgcaacg ggttttccgt ttaccaagac
     2641 tgtgagggtg ggctctgcgc attccagacc gttgtctaga gcggatagct ttaagagtat
     2701 tccgccggcc tcaacggcag caccagcccg cacgtcgggt ttgccgctat cgccggactt
     2761 tccaggcgaa ggtgtccatg accatgaggg atgcccttcg atgcgctcta tcatcagcac
     2821 actccttgga attactccac aagaaagcag gacttgcact aaaatgcagg ctgaaagggc
     2881 aaggcctgta agggcggaga atacaatggt cgctacatcc caattttgca aaggtctagc
     2941 cactccttct tgacattgcg gcgctcattc ctgccagacg tcctgtggag aacgcggctt
     3001 ggagattgta gcctccggta ggggcgtcaa tatcgagaac ctccccacaa aagtacagtc
     3061 ccggcaatag tttcgactcc agcgtgcgtg ggtccacttc ccggagcgct attcctcccg
     3121 aggtaactat ggcttcctca atagggcggg ttttcagcac ggttaaaggc attcgtttta
     3181 gcgtctcgac caatgccgat ctttgggcag ttgtaatctg gtttgcaggg atagtctcgt
     3241 ctacctgtgc aaggcttatt acaaccggaa taatcgagtg tggaagaagc atccccaagg
     3301 agtttttgag ctgttttttg gatgcggcca cgagatctcg ctgtattcgg cgatggaggg
     3361 tggcctcatc taaggcgggt tttagatcta tggagagcgt gaaacggcca ctctcgatcg
     3421 cttccgactc cctgagagat ctgcatactg cgcgactcaa gctgaggacg attggtccgc
     3481 tcacaccgaa gtgggtaaag agcatttccc caaattccct acctatcctg cgtcctctgt
     3541 cgtaaaggct tagctctacg ttgcgcagac tgaggcctgc gactgatcct ggccaatctt
     3601 ctactgtttc catgggaacg agtgcaggca aaggctgcac gatagtgtgc cctgcctcgg
     3661 cggcaatgcg gtagccatcc cctgtgcttc cggtgcccgg atacgagatt ccgcctgttg
     3721 cgattatcac ggcgtctgcc caagaattgt tcccgccgga gtcgattacc cccttgactt
     3781 tgccgtcggc gactgcgagc ccgg
//
LOCUS       DAOSAI010000248         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15341_length_3784_cov_2.863770, whole genome shotgun sequence.
ACCESSION   DAOSAI010000248 DAOSAI010000000
VERSION     DAOSAI010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15341_length_3784_cov_2.863770"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..373
                     /gene="rplU"
                     /locus_tag="PLM83_08240"
     CDS             59..373
                     /gene="rplU"
                     /locus_tag="PLM83_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOK71429.1"
                     /translation="MYAIIETGGKQYRVEPGQVVRVEKLDAEVGDSIEFHRVLMVSGD
                     DGIKVGSPVVDGARVKGAILEHGKAKKIYVFKYMAKKRVCTKTGHRQPFTAVKIESIS
                     VA"
     gene            411..758
                     /locus_tag="PLM83_08245"
     CDS             411..758
                     /locus_tag="PLM83_08245"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOK71430.1"
                     /translation="MVKLHVRRVEGHVVRFALHGHAECGEYGYDLVCAATSALTIAAA
                     NGLFEHAGVSRAVRYRSGYLDCVILNCPSAEVRMRADAVLETMISGIRAIALEYPESV
                     SVVDIGGSVSARY"
     gene            837..1157
                     /gene="rpmA"
                     /locus_tag="PLM83_08250"
     CDS             837..1157
                     /gene="rpmA"
                     /locus_tag="PLM83_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOK71431.1"
                     /translation="MIRMDLQLFAKKKGVGSSRNGRDSRSQRLGVKKFGGQYVSAGNI
                     IVRQCGTKVHPGINVGMGKDYTLFALADGYVQFEHNGKRSKKVSVVPELPVAEEPAHV
                     VESM"
     gene            1251..>3784
                     /locus_tag="PLM83_08255"
     CDS             1251..>3784
                     /locus_tag="PLM83_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOK71432.1"
                     /translation="MGKIPVWARVLIVAIIIVAVGWKTISNITTEYLWYQDLGYVSVF
                     WKCLEARVAVAAAIFAVTFAFVAANLLSMKSASKAMPRRSALFLATIAGVLTAGFSTD
                     LWMEFQQFVHATPFGKADPIFGQDVGFYVFRYPFLKEVYKGAIWVVGLSATFVILVYL
                     FSGSLIRFSREEVEEPAIGSRRRRPRRGRLLLEQADPGTKLHIGILLAIVFCVVAFGY
                     WLDMRGLVYSERGVGIGASYADVHGALPGMRLMLFMSLAAAVIMIISAISQTRVKTLA
                     WMAATVVIMGAFSLLAVNIYPNLVQRLYVTPNELRAEREYISHNIDFTRSAFGLDSIE
                     EKEYVVDQNLSWTDVNSARATLDSIRLWDWEPLLATYGQLQGMRLYYQFNDVDVDRYL
                     IDGNLVQVMLAARELDVESLPPQAQTWVNRRLKYTHGYGVCASPANEIGPDGLPTFLL
                     RDIPPKGSDSLEIERPEIYFGELTRDWVVVNTKTEEFDYPVGDTNKSTRHMGDSGVRI
                     SNPLRRAMFATKFSDARLLLSSDITSDSRILFDREIGQRARKIVPFLSYDSDPYIVVA
                     DGRLIWILDGLTTSDRYPFSERYVGGINYIRNSVKVTIDAYTGQTDFYLIDRNDPVAV
                     TYAKIFPGLFKEADEMPQSIREHVRYPEDLFTIQAKMYGMYHMRDPEVFYNKEDLWAI
                     PNELYGGSEREVSPYYIRLVLPGESDMGFVLFVPFTPSRKDNMVAWLAVNSDMDDFGR
                     MVAYKFGKQKVIFGPMQIEAQIDQDAEISKMLSLWNQSGSTVIRGNLLVYPIGGGLLY
                     VEPLFLAAQTSQLPQLKRVVLCDGTKVAIGSDLWSALDAMLSGHGEG"
BASE COUNT          866 a          835 c         1110 g          973 t
ORIGIN      
        1 gccttctgtt tttctctttt atcccggcga gcccagagtg cgaggaggta aaaggatagt
       61 gtatgcaatt atcgaaaccg gcggaaaaca gtaccgtgtt gagccggggc aggtcgtccg
      121 tgtcgagaag ctggacgctg aggtcggcga cagtattgaa ttccaccgtg tgcttatggt
      181 gtcaggcgac gatggaatca aggtaggtag tccagttgtt gacggtgcaa gggtcaaggg
      241 cgccattctt gagcatggta aggccaagaa gatctatgtt ttcaaatata tggctaaaaa
      301 acgagtctgc acgaaaacag gacataggca gccgtttaca gcggtaaaga tcgaatcgat
      361 ctcggtggca tagtttgctc tgatgagcgc ggtgcaagaa ggtttttgcc gtggttaagc
      421 ttcatgttcg gcgtgtggag ggccatgtgg ttcggtttgc cctgcatggg catgcggaat
      481 gcggagaata tggctatgat ttggtatgtg ctgctacgtc agctttgacg attgccgctg
      541 caaatgggct ttttgagcat gcaggtgtgt ctcgtgccgt aaggtatcgt tccggatacc
      601 tggattgcgt gattttgaat tgcccatctg cggaggtgcg tatgcgagcc gatgccgttt
      661 tggagaccat gatttctggg attcgagcca tagcgctaga gtatccagaa agtgtttccg
      721 tcgttgacat cggaggttcg gtctcggcgc gatattgagg agaaagatgc gccttggcgt
      781 ttggcgtagg cgtgtgagtt aaggcgaaat gaaacggaat ccgggaggtg tttccaatga
      841 tcagaatgga ccttcagcta tttgcgaaga agaagggagt cggaagctcc cgaaacggtc
      901 gtgactcccg ttcgcagagg ctcggagtta agaagtttgg cggccaatac gtttccgccg
      961 gcaacatcat tgtcaggcag tgtgggacta aggttcaccc tggtatcaat gtggggatgg
     1021 gtaaggacta taccctgttt gctctggcag acggttatgt tcagttcgag cacaacggaa
     1081 agcgaagcaa gaaggtttca gtcgttcctg agctgcctgt tgccgaggag ccagcgcatg
     1141 tcgttgaatc tatgtagttg gtgattggag gctgaagggc tcgcatcagg tgtgcttgat
     1201 gtgggccctt ttgttatcgc tgtgctactc cttctgagag ggtgaggttg atgggcaaga
     1261 taccagtctg ggctagagtc ttgatagtgg ccattattat cgtggcagtc gggtggaaga
     1321 cgatttctaa tataacgact gagtatctgt ggtaccagga cttggggtat gtgagtgtat
     1381 tctggaagtg cctggaagcg agggttgctg tcgcagcagc gatattcgcc gtgacatttg
     1441 cctttgttgc tgcaaatctt ctgtcaatga aatcggcgtc gaaagctatg ccaagacgat
     1501 cggcactttt cctggctaca attgcgggag tgttgactgc cgggttttct acagatttgt
     1561 ggatggaatt ccagcagttt gtgcatgcta cgccgtttgg gaaagcagac ccgattttcg
     1621 gccaagatgt aggcttctac gttttccgat atcccttcct taaagaagtc tacaaaggcg
     1681 caatatgggt tgtgggcttg agcgctacct ttgtcattct tgtctacctt ttttccggat
     1741 cgctgatcag atttagccga gaagaagtgg aagaacccgc aataggcagc cgccgcagga
     1801 ggcccaggcg aggaaggctt ctcttggaac aggctgatcc cggcacgaag cttcacatag
     1861 gcatacttct tgccatcgtt ttttgtgttg tggctttcgg ttattggctt gacatgcggg
     1921 gattggtcta ttccgagagg ggagttggca taggcgcaag ctatgctgat gtacatggcg
     1981 ctctaccagg catgcggctt atgctcttca tgtccctcgc agctgcagta atcatgataa
     2041 tctctgccat atcacagact cgtgtaaaga cattggcatg gatggctgct acggtagtga
     2101 tcatgggagc cttttcgcta ctggctgtaa atatctatcc caatctagtc cagcgcctct
     2161 atgtgacgcc caacgagttg agggcagaaa gggagtacat ctcgcacaac atcgacttca
     2221 ccagaagtgc ttttggtctc gacagtatcg aagagaagga atacgttgtg gatcagaacc
     2281 tctcctggac cgacgttaac tcggcacggg ccactctcga cagcataagg ctttgggact
     2341 gggagccatt gcttgcaact tacgggcaac ttcagggcat gcggctttac taccagttta
     2401 acgatgtgga cgttgatagg taccttattg acggcaacct tgtccaggtt atgctggctg
     2461 caagagagct cgacgtggaa agtctgccgc ctcaggcgca gacatgggtc aacaggcgtc
     2521 tcaagtacac gcatggctac ggcgtgtgtg cgagtcctgc gaatgagata ggccctgacg
     2581 gacttcccac attcctattg cgagatatac cgcctaaagg ttctgatagc ctcgagattg
     2641 agcggcctga gatatacttc ggtgaactta ctcgcgattg ggttgtcgtc aatacgaaga
     2701 ctgaagagtt tgactatccg gtaggtgata ccaataagtc tacccggcac atgggtgact
     2761 ccggtgtccg catttccaat cctctgcggc gcgcgatgtt cgccacgaag ttttccgacg
     2821 ctaggctgct gttgtcgtct gatatcactt ctgacagcag gatattattt gatagggaga
     2881 ttgggcagcg tgctagaaaa atagtcccat tcctcagcta tgacagtgat ccatacatcg
     2941 tagttgctga cggtaggctg atatggattt tagatggcct taccacgtca gaccgatatc
     3001 ctttttccga aagatatgtg ggaggcatca actacatcag gaattccgtg aaggtcacga
     3061 ttgatgccta taccggccag acggatttct atctcatcga cagaaatgac ccggtggcgg
     3121 ttacgtacgc aaagatattc ccgggactgt ttaaggaagc cgacgaaatg ccgcaaagca
     3181 tacgtgaaca cgtgcgttat ccagaagacc tcttcacgat tcaggccaag atgtacggga
     3241 tgtaccatat gagagatccc gaggtgttct acaacaagga agacctctgg gcaattccaa
     3301 atgagcttta cggtggttct gaacgcgaag tgagccctta ctatatccga ttggtgcttc
     3361 caggtgagtc cgatatgggc ttcgtgcttt tcgtgccgtt cacgccttca cggaaggaca
     3421 acatggttgc atggctcgcg gtaaacagcg atatggatga tttcggccgg atggtggcgt
     3481 acaagttcgg aaagcaaaag gtcatatttg gcccaatgca gatagaggcc cagatagacc
     3541 aggacgcaga gatctcaaag atgctgtcgc tgtggaatca gagcgggtca actgttattc
     3601 ggggcaacct gttggtgtat cctattggcg gcggattgct gtacgtggaa ccgctatttc
     3661 tggcagctca gacaagccag ctgccccagt tgaagcgcgt ggtgctatgc gacggaacca
     3721 aggtcgccat aggaagcgat ctgtggagcg ctcttgacgc gatgctttcc gggcatggcg
     3781 aagg
//
LOCUS       DAOSAI010000249         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15373_length_3778_cov_3.719850, whole genome shotgun sequence.
ACCESSION   DAOSAI010000249 DAOSAI010000000
VERSION     DAOSAI010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15373_length_3778_cov_3.719850"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /gene="gatB"
                     /locus_tag="PLM83_08260"
     CDS             complement(<1..880)
                     /gene="gatB"
                     /locus_tag="PLM83_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOK71433.1"
                     /translation="MTSDYEVVVGIEVHVELATEAKVFCGCRTAFGAEPNTQTCPTCL
                     GMPGALPVLNKRALEFAIRAALALNCSVADYTKFDRKNYFYPDLPKGYQISQYDCPIG
                     SNGYMDVDVNGESKRIRIKRVHLEEETAKLMHAGDDIISASHSLLDFNRSGIPLIEIV
                     SEADMRSADEARAYLTKLRSILLAIGVSDCKMEEGSMRCEANISVMPRGSEQYGTLVE
                     LKNIASFRAVHRAIQYEAARQIEIVSSGGEVARETRHWDDVKGETAFMRGKEEAHDYR
                     YFPEPDLGPIVIDRAWV"
     gene            complement(877..2379)
                     /gene="gatA"
                     /locus_tag="PLM83_08265"
     CDS             complement(877..2379)
                     /gene="gatA"
                     /locus_tag="PLM83_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOK71434.1"
                     /translation="MKLCDMTIHELAALLRAGEVTSTEITESVFDRIDQVEESVGAYV
                     TTARDQAFEQAAAADKRLLQARKRGSLSDISPLMGIPLALKDNICTRGIRTTCASKML
                     ADFVPPYDATVARRLGDLGAVIVGKTNMDEFAMGSSTENSAFHVTRNPWDFERVPGGS
                     SGGSAAAVAAGEAIGALGTDTGGSVRQPASFCGLVGLKPTYARVSRYGVVAFSSSLDQ
                     VGPITRDVWDCAIMLRAIAGHDERDATSSPEPVPDYCGFLKETDRDFTIGVPSEFMHS
                     RLDSGIRELIDNAVRALEDAGARIVEVSLPHSDYAIAVYYLIATAEASSNLARMDGIR
                     YGFRAGDAHGVQAVIRQSRSEGFGPEVKRRIMLGTHALSSKHYDEYYLKALKARTLIL
                     GDVKEVLEKCDCIISPTSPTVAFRIGEKLDDPVALYLMDVYTTIANLSGMPAVSVPCG
                     TLKGLPVGAQLIGRAFDEGTLFRIAHLLEQACGESVKRPPLEAMKGGRDR"
     gene            complement(2376..2690)
                     /gene="gatC"
                     /locus_tag="PLM83_08270"
     CDS             complement(2376..2690)
                     /gene="gatC"
                     /locus_tag="PLM83_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOK71435.1"
                     /translation="MSELSISKEETAHIAELARLSLNESELAQAQHDLSEIIDYMNKI
                     AGLNTDGVALTIQPCPVANVWREDVVLPSLNREEVLANAPDRQGEFFRVPRIIEAEGA
                     DE"
     gene            complement(2705..>3778)
                     /gene="ligA"
                     /locus_tag="PLM83_08275"
     CDS             complement(2705..>3778)
                     /gene="ligA"
                     /locus_tag="PLM83_08275"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOK71436.1"
                     /translation="TKIKDIKVQVGRTGALTPLAILEPVRLAGSVVSRASLHNEDYVR
                     AKDVRVGDAVIIQRAGDVIPEVVRVLVEERTGDEKEFEMPRVCPACGSEVLREAGEAA
                     TRCISLSCPAKIREGMIHFASKGAMDIEGLGPSTIDRLLDAKLVADVADIYGLRLDQL
                     AGLDRMGEKSAANLVASIEQSKSNPLYRVIFALGIRHVGENVAKLLASHFHSMAALIA
                     AERDDLLAIPTIGPAIADSVVSFFSSEMNLETVKKLAAAGVRMSDELEEAKPAAVEFD
                     GKSFVFTGTLASLTRQKAEEAVENLGGRVSSSVSRKTDYVVAGENAGSKLARARELGV
                     AVLSEQDFAEMLERAGCRLGDER"
BASE COUNT          850 a         1197 c          845 g          886 t
ORIGIN      
        1 ccacccatgc cctatcaatc acgatcggac caaggtcggg ttcggggaaa tagcgatagt
       61 catgcgcctc ttccttcccc ctcataaacg cagtctcgcc tttgacatcg tcccaatggc
      121 gagtctccct cgccacctct ccaccagagc tcacaatctc tatctgcctc gccgcctcat
      181 actgaatggc cctgtgcacg gcccggaaag aggcgatgtt cttcaattct accaacgtac
      241 catactgttc agacccgcga ggcatcaccg agatgttagc ttcacacctc atggaaccct
      301 cctccatctt gcagtcagat actccgatag caagcaatat ggagcgaagc ttagtgaggt
      361 aagcccttgc ctcatcagcg gacctcatgt ccgcctcaga tacgatctcg atcagtggaa
      421 tccccgacct gttgaaatcg agcagcgaat ggctggcgga aataatgtca tctccggcat
      481 gcatcagttt ggccgtctct tcctcgagat gaactctttt gatccttatc cgtttggact
      541 cgccattgac atccacgtcc atgtatccat tgctccctat tgggcagtca tactgggata
      601 tctgataacc tttgggcaag tcaggataga agtagttctt tcgatcgaat ttagtatagt
      661 ctgctacaga acagttgagg gccaaagcag cgcgaatcgc gaactccaat gccctcttgt
      721 tgagcactgg aagagcgcca ggcatgccca agcatgtggg gcatgtttgg gtattcggct
      781 cggccccgaa agctgtcctg cacccacaga acaccttggc ttcagtcgcc aactcgacgt
      841 ggacttctat tccaacaaca acttcataat ccgaagtcat ctgtcacgcc cccctttcat
      901 ggcttcaagt ggcggccgct ttaccgactc gccgcatgcc tgctccaaca gatgggcaat
      961 gcgaaatagt gtgccctcgt caaaagccct ccctataagt tgggctccga cagggagtcc
     1021 tttaagggtc ccgcatggca cggataccgc aggcatgcct gaaaggttag ctatggttgt
     1081 gtaaacatcc atcaaataca gggccacggg gtcatctagc ttttcgccta ttctgaacgc
     1141 caccgtcggc gacgtcggcg agatgatgca atcacatttc tcgagaactt ctttcacgtc
     1201 acctagtatt agagttcgtg ccttcagtgc cttgagatag tactcatcat aatgcttcga
     1261 gctcaacgcg tgggtgccca acatgatgcg gcgcttcacc tcgggaccaa acccctcgct
     1321 tctgctctgc cttatcactg cctgaacgcc atgagcatcc ccggctcgaa agccatacct
     1381 aatcccatcc atacgcgcca ggttcgagct ggcctcggcg gtggcaataa ggtagtagac
     1441 tgcaatcgca tagtccgaat gtggcaacga tacctccaca attcgcgctc ccgcgtcttc
     1501 cagagcacgc actgcattat cgatcagttc cctgatgcca ctatcgagcc ttgaatgcat
     1561 gaattcggaa ggcaccccaa tagtaaaatc tctgtctgtc tctttcagaa atccgcagta
     1621 atcaggaacc ggctcaggtg aagacgtcgc gtcacgttcg tcatggcctg ctattgctct
     1681 caacatgatt gcacagtccc aaacgtcccg ggtaataggg ccaacttggt ctagagatga
     1741 cgaaaatgcc actactccgt atctgctcac ccgtgcatac gtcggcttca atccgacgag
     1801 tccgcagaat gaggcgggtt gccgcactga accccctgta tctgtcccta aggcgcctat
     1861 ggcctctccg gccgccaccg ccgccgctga gcccccgcta gagcccccgg gcaccctctc
     1921 aaaatcccac ggattccggg taacgtggaa tgccgagttc tccgttgatg atcccatggc
     1981 aaactcgtcc atgttggtct tccctacaat tactgcgccc aagtccccga gacgcctggc
     2041 tacagtcgca tcgtatggcg gcacaaaatc cgcaagcatc ttcgaagcac acgttgtcct
     2101 gatgccccgg gtacagatat tgtccttcaa tgccaatgga atccccatca gcgggctgat
     2161 atcagagaga cttcctcttt tccgcgcttg aagcagtcgc ttatctgccg cagctgcctg
     2221 ttcaaacgcc tggtcgcggg cggtggtcac ataagcgcca actgattcct ctacctgatc
     2281 tatccgatca aagacagact cggttatctc cgtggatgtg acttctcctg cccgaagcaa
     2341 tgctgcaagc tcgtgaatgg tcatgtcgca cagcttcatt cgtctgcccc ctctgcctcg
     2401 attatccttg gcacccggaa gaactcgccc tgcctgtcgg gggcattggc aagtacctcc
     2461 tcacgattga gcgagggcag aaccacgtcc tcgcgccata cattggcgac cggacaaggt
     2521 tgaatggtca gcgccacccc gtccgtattt agcccggcaa ttttgttcat gtaatcgatt
     2581 atctcagaaa ggtcatgttg tgcctgagca agttctgatt cattcaggct caacctagcc
     2641 aattcggcaa tatgggctgt ctcctccttt gaaatgctca attcactcac caccatcaca
     2701 aggattatcg ttcatcacca agacggcatc ccgcccgttc caacatctca gcaaaatcct
     2761 gttcagacag gacagcaacc ccaagctccc tagctctggc aagtttggac cccgcgttct
     2821 ctcctgccac aacataatcc gtcttccggc tcacactcga acttacgcgc ccgccaaggt
     2881 tctctactgc ttcctccgct ttctgccttg tcaggctcgc aagcgtacct gtgaaaacaa
     2941 aactcttgcc gtcaaactcg acagccgccg gcttggcttc ttcaagttca tccgacatac
     3001 gcacgcccgc tgcagccaac ttcttcacgg tctccaggtt catctcagat gagaaaaacg
     3061 agactacgct gtcagcgatc gccggcccta ttgtaggaat cgcgagcaga tcgtcacgct
     3121 cggcggcgat aagcgccgcc attgaatgaa aatgtgaagc cagcagtttc gccacattct
     3181 cacccacatg ccttattccc agggcgaaaa tgactcggta gagcggattc gacttgctct
     3241 gctcgataga agccacgagg tttgccgctg acttctcacc catcctatcc agccccgcga
     3301 gctgatccaa cctgagccca tatatgtcag ctacatcagc gacgagtttc gcatcgagca
     3361 acctgtctat tgttgacggc ccaagacctt caatatccat ggcgcctttc gaagcaaaat
     3421 gaatcatccc ctcacgaatc ttcgcagggc atgatagact tatgcagcgc gtagccgcct
     3481 ctccagcctc ccgtagaacc tcagaaccgc atgcagggca tacccgcggc atctcaaatt
     3541 ccttttcgtc tccagttcgt tcttcaacca atacccgtac tacctctgga atcacatccc
     3601 cggcccgctg gattataacg gcatccccca cacgcacgtc ctttgcccgc acataatcct
     3661 cattatgaag agaagccctg gatacgactg atcccgccag cctgactggc tctaaaatcg
     3721 caagcggggt aagcgctcct gttctcccga cctgcacttt tatatccttg atcttcgt
//
LOCUS       DAOSAI010000250         3774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15393_length_3774_cov_4.624092, whole genome shotgun sequence.
ACCESSION   DAOSAI010000250 DAOSAI010000000
VERSION     DAOSAI010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15393_length_3774_cov_4.624092"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..991)
                     /locus_tag="PLM83_08280"
     CDS             complement(68..991)
                     /locus_tag="PLM83_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HOK71437.1"
                     /translation="MKHVVSVSIGSSSRNHSARITLLGEEILVERIGTDGDVAKAKQL
                     IGELKDKVDAFGLGGIDLYLEAKGRKYYFREAKNIAAMAGDTPVVDGTGLKSRVEYNA
                     VMSVATEWGLPIAGKDVFLVCVFDRIGMGLAFEELGCRMTYGDLMFATGIPIPLYKIS
                     SVHALAPIVMPWLTQLPFSWVYPTGKSQRANRDKAKKRKLKFQQYYNRAHVLAGDFHY
                     IYRHLPERIDGKIIVTNTVTEDDVEDIGRRGAHALVTTSPEIQGRSFGANLMEAMLIA
                     LIDKPIDEITDEDYDDMMKRLDLRPRVVMYD"
     gene            1683..2147
                     /locus_tag="PLM83_08285"
     CDS             1683..2147
                     /locus_tag="PLM83_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71438.1"
                     /translation="MWNELAVEAITILLWAATALVVLFLSGLSKYLQEQARRVENDAA
                     RGLIQSALSEAEKVGIDAVQATNQVFVDAIKASSADGKLSKEDAATAMKIAVEYFKTH
                     LSSRSYQVLSEPDGKLDDKWISDFLEARLAQTKAESSRCAYPLPAPLPDCPE"
     gene            complement(2589..3347)
                     /locus_tag="PLM83_08290"
     CDS             complement(2589..3347)
                     /locus_tag="PLM83_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOK71439.1"
                     /translation="MEKKQVAAELDRILRLDTKPVGIKLYKNQEDLPRKPFNFKLNLC
                     QLVAMARYQGKTNSGTPDKMICAMGAACVGLIDTPEAISSGKAAVGAYVKDQTAGKTF
                     MDNTFKIGDTGKRYDGILVGALESMEEDPDVVVVYVNPAQAMRLIHACAYETGEKITA
                     DTVAEAALCSCIGYAVANDKPVVGFPCAGDRIFGGTQTHEVVFVAPYPLVREKLVENL
                     ERTAEGGFSVYPVPPNMFWTPTMPPTYTIGPEDL"
BASE COUNT          914 a         1032 c          911 g          917 t
ORIGIN      
        1 cgcccgggct acatggcctc ggaacgcagg caataaggaa gcaaaaagac atggaataag
       61 ccaacgatca atcgtacatt accaccctgg gcctaaggtc aagtcttttc atcatgtcat
      121 cataatcctc atcggtgatt tcatcaatcg gcttgtcgat caaggcaatg agcatggcct
      181 ccatcaggtt tgcaccaaac gaacgtccct gtatctcagg gctggtggtg acgagggcat
      241 gagcgccacg ccttcctatg tcctcaacat cgtcttcagt caccgtattg gtgacaataa
      301 tcttcccatc gatccgctca ggcaggtgcc tgtatatata gtgaaaatct cctgcgagca
      361 cgtgggctct gttgtagtat tgctggaatt tgagtttgcg cttcttcgcc ttgtccctgt
      421 tggcccgttg ggacttgcct gtaggatata cccagctgaa tgggagttga gtcagccacg
      481 gcataacaat aggggctagc gcgtggaccg aactgatctt gtacagcggt ataggtattc
      541 ccgtcgcaaa catcaggtct ccataggtca tgcgacaccc aagttcctcg aaggcaagtc
      601 ccattccgat gcggtcgaac acacagacca ggaagacgtc ctttcctgct atgggcaagc
      661 cccactcggt agcgacagac atcacagcat tgtactccac cctgctcttc agcccagttc
      721 catctacgac aggggtatct ccggccatgg cagcaatatt cttagcttct cgaaagtaat
      781 atttcctgcc ttttgcttcg agatacaggt cgatcccgcc aagtccgaaa gcatcaactt
      841 tatccttaag ctctccaata agctgtttgg cttttgcgac atcaccgtct gttcctatcc
      901 gctctacaag aatttcctct cccaacaacg taatcctggc tgagtggttt cgagaagatg
      961 agccgatgct tacactaaca acatgcttca tgattccatt ctccctcctt caaccggctg
     1021 aagcgtgcgt atcccccccc caataatcag tctattcgcc ctgtatggcg gaccatgtgc
     1081 ctgtaactga acacaccgcc ccttccacac agactcgtct tcgtcctaaa tgaggctgca
     1141 caggaaatgg acgctgtaga accctgaaaa cctgcgtagc tccgaaaaag catttgggct
     1201 ttttcaagta gctgagaact gaacagcaag caacgctaat gtggacaagc cgatcagaag
     1261 ttgctcagcg caaggcagct cagatgggga tgatcagtag cacataacgc agcggtcatt
     1321 gtctaaaccg acatctaaat ggtgtacaca tatttgtgca catattgatt ctaccaaata
     1381 ggctttggag accgcaagat aattggccca ttggtgatct aagccgcgag gagctggcgc
     1441 cagtcgccct agacgatcga ctcacggtca attttcttcg ggcgtggcta gcctgcagac
     1501 agtcattctt cccacgaagt gttgcagatt accaaagaac cttacatttc gtgttgctag
     1561 ttgaaggttt ttctgccatg ccggagaagg atgagcaatg gcatgatttt gctcctaaat
     1621 ctgttcatgg gattcttctg ccattatcac tctttccaag caattcggag gtgtgatctg
     1681 acgtgtggaa tgagctagcg gtcgaggcaa tcacgatact attgtgggct gcgacagccc
     1741 ttgttgtgtt gttcttgagc ggcctttcca agtaccttca ggagcaagca aggcgggtgg
     1801 agaatgatgc agcgcgagga ctaatccagt cagcgctctc ggaagcagaa aaggttggca
     1861 ttgatgccgt tcaggcaaca aaccaagtat tcgtcgatgc cataaaggca tcttctgcag
     1921 acggcaagct ttcaaaagaa gatgcagcta ccgcaatgaa aatagctgtt gagtatttca
     1981 aaacccacct gtcatcccga agctaccagg ttctcagcga acccgatggc aagctcgacg
     2041 ataaatggat aagcgacttc ttggaagcaa gattggcgca gacaaaagca gaatcctctc
     2101 gctgtgctta tccccttccc gcaccgttac ctgattgtcc cgagtaacaa ggtcaaggct
     2161 agggggcggc aggcacactg tcgcctcgtc tgccccgggc tcggctacgg ctgccaatgc
     2221 acaagacccg gagaggccat ggtcaccaag ggagcgatag ctcgacgcct agttcaccgg
     2281 attacaaacc aagtccccta tctgcaggag ctgacggatg acgggaccga gactacaacc
     2341 ggcctggccc tgcgcaacag gatgctctgt gattaagcaa gccctgtacg cagggcccga
     2401 agctgaaaga tcttagcctc tcaatattca agggtaggaa gaaaggcggg catttgcgca
     2461 gatgcagata ttgtcttgcc ttttatagca cgtctctggc tggaatggga aaacgcagaa
     2521 gcaaagaaac agggggcggg atgcccgtcc cctcagttct gcgctcgcct ctgtccggtt
     2581 ttccgcttct acagatcttc cggcccaatg gtgtatgtcg gaggcattgt tggtgtccaa
     2641 aacatgttcg ggggcacagg atagactgag aagccgccct cagccgtgcg ctccaggttc
     2701 tctaccagct tctctctgac caacgggtat ggcgccacaa acactacttc atgggtctgc
     2761 gttcctccga agatcctgtc accggcgcac gggaatccaa ctaccggctt gtcgtttgcc
     2821 actgcatagc cgatgcagga gcaaagggcc gcttccgcca cggtgtctgc agtaatcttc
     2881 tcgccagtct cgtatgcaca tgcatggatc aacctcatag cctgagctgg attgacatac
     2941 actacgacca catcaggatc ctcttccatg ctctcgagag cccctacgag aatcccatcg
     3001 tatctcttgc cggtatcccc tatcttgaag gtgttgtcca tgaaggtctt gcctgctgtc
     3061 tgatccttca cgtatgcgcc gacagcagcc ttgcctgatg agatggcttc aggggtgtca
     3121 ataagcccta cacaggccgc gcccatggca caaatcatct tgtccggtgt accggagttc
     3181 gtctttccct ggtaacgggc catggcaacc aactggcaca agtttagctt gaagttaaaa
     3241 ggcttcctcg ggagatcctc ctgatttttg tatagtttga tccccactgg cttggtgtca
     3301 agacgtaaaa tcctatcgag ttccgctgca acttgcttct tctccatgta aaatgtctcc
     3361 ttccataaaa actgcagaca tttgcctgta cactcaatct cgaatcgctc aatcgcgacc
     3421 ttacatatat cattatgcgc ctgattgtga aaaatatcac gttatctcac aggcaacagg
     3481 tcccgctcgg ccaacatgcg cggtatcgta tccaccagag ggcttgttta gacatgggta
     3541 gtgaaagaac aggcgattgc ggaagtcgat catacagtag ttaatatggg caccgcttat
     3601 ggaaggcatt accggagtcc cctgcaaatc ccgtggtcac atgagcttct ttctcaatca
     3661 gaaaggcaga ccgcagccag cttatattta ctcctagatt gcccgctgcg gtcgatgtat
     3721 ttttcgtaag ctacttccaa gaagccttca ctgcttcaac gagctccacc gtac
//
LOCUS       DAOSAI010000251         3767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15436_length_3767_cov_3.342942, whole genome shotgun sequence.
ACCESSION   DAOSAI010000251 DAOSAI010000000
VERSION     DAOSAI010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15436_length_3767_cov_3.342942"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..676)
                     /locus_tag="PLM83_08295"
     CDS             complement(47..676)
                     /locus_tag="PLM83_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK71440.1"
                     /translation="MRVAAIIPAYNEGPRIASVLDVVRASPLVDEIIVVDDGSDDDTA
                     EVARAHGANVIRLGENTGKGGAVARGLEATLGDVILFLDADLIGLTVDHVNALIEPVC
                     SGEADMTIGIFGNGRTATDLAQALTPWLSGQRAIKRSVLEGLELDISRFGVEAVLTRH
                     AKESGLKVVDVKLPRLTHVMKEEKMGTVKGMASRARMYWEVLKSIVDWD"
     gene            complement(689..2428)
                     /locus_tag="PLM83_08300"
     CDS             complement(689..2428)
                     /locus_tag="PLM83_08300"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOK71441.1"
                     /translation="MRMSQMFAPTLREVPSEADIPSHQLMLRAGMVRKTVSGVYSYLH
                     LGCRVVRKIEKIVREEMDRQGAQEVLMPAIQSADIWRETGRWDDYGSEMFRLKDRNGR
                     EMCLGPTHEEIITVTVRDEIRSYRQLPKILYQIQTKYRDEIRPRFGVMRSREFIMKDA
                     YSFDRDNEGLDRSYEKMYEAYCRVFSRCGVDYRVVEADAGAIGGSQNHEFMVVSEVGE
                     DALVFCDACGYAANVERAESAKPAERVADTEKVRTGIRKISTPGMRTIEEVTGYLKVE
                     PRVLLKTLVYVADGTPVAAIVRGDRDLNEAKLKRVLGCRELELATPEVIERVSGAPVG
                     FTGPVGLRERIKIVADWEATVVEDAVVGANAVDAHLVGAEYGVDYTADIVADIRNVVA
                     GDACPRCGAELCGARGVEVGHLFKLGTKYSSALGAKYLDEGGVEHPIIMGCYGIGITR
                     TVASVIEEHHDDDGIIWPMSVAPYQAVVMPLNTSESAQVEAAERIYSELCKHGVEAVM
                     DDRDERPGVKFKDADLIGFPIRVIVGPKHLGDGEVEVSMRRDGTVELVKIENVAVRVK
                     EIVDRELAALMPE"
     gene            complement(2451..3530)
                     /gene="ispG"
                     /locus_tag="PLM83_08305"
                     /gene_synonym="gcpE"
     CDS             complement(2451..3530)
                     /gene="ispG"
                     /locus_tag="PLM83_08305"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOK71442.1"
                     /translation="MATREETRQVMAGSVPIGGGAPISVQSMTTADTRDADAAIKQIR
                     QLAAVGCEIVRVAVPDEEAADSLRSIRKASSVPLVADIHFNYKLALASIEAGVDKLRI
                     NPGNIGGADRVREVAHAAADRGIPIRIGVNAGSLPADLVSKYGRTPEAMVEAALRHVS
                     LLEREGFESIVLSLKASDVKRTTEAYRLIARRAPYPLHVGITEAGPPPGGLIKSAIGI
                     GMVLSEELGDTIRVSLTGDPRDEVIAGREILASLGMRRFGPTIVSCPTCGRCRTDLAG
                     IVKEVKDGLSHLDAPLVVAIMGCEVNGPGEASEADVGLACGNGVGLIFSGGRVVRKVR
                     EAEMVGALVEAAESMAANRHEKSHG"
     gene            complement(3570..>3767)
                     /locus_tag="PLM83_08310"
     CDS             complement(3570..>3767)
                     /locus_tag="PLM83_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOK71443.1"
                     /translation="GLLNLMPIPFLDGGWVLFLIIEAVRGKPLSPQVEGFLRFIGLAL
                     LMLLLVYATVSDISRIMVQRM"
BASE COUNT          858 a         1255 c          809 g          845 t
ORIGIN      
        1 gacaaatacc gtagacaaca actaccctcc agcaatccgg cgggccctaa tcccaatcaa
       61 cgattgattt gaggacctcc cagtacattc gggcacgtga ggccataccc tttacagtgc
      121 ccatcttctc ttctttcatc acatgagtca gtctcggaag cttcacgtca accaccttga
      181 gcccggattc cttggcatgt cgcgtcagca cggcctcaac tccgaatctg gaaatgtcga
      241 gctccaaacc ttccaacacg ctgcgcttga tagccctctg ccccgacagc caaggcgtca
      301 aggcctgcgc aaggtcggtg gctgtgcgtc cattgccaaa aatcccgatc gtcatatccg
      361 cttccccact gcaaaccggt tcgatcaggg cgttcacatg atcaacggtc aacccaatga
      421 ggtcagcatc tagaaagaga atcacatctc ccagtgtagc ctcgagtccc cgcgctacag
      481 ccccaccttt gcccgtattc tccccaagcc ttatgacatt cgctccatgg gctctggcta
      541 cttcagcagt atcatcatca gagccatcat ctacaactat gatctcgtcc accagagggc
      601 tcgcacgcac gacatcaagc actgacgcaa ttctaggtcc ctcgttgtac gcgggaatga
      661 ttgcggctac acgcatgctt gtcacctcct actcgggcat gagcgctgca agttcacgat
      721 ccacgatctc tttgacacgc actgcaacat tctcgatctt tacaagctca acggtgccgt
      781 cacgcctcat gctcacctca acttcgccgt ctccgaggtg tttgggccct actatcacgc
      841 gaataggaaa tccgatcaaa tcggcgtcct taaacttcac ccccgggcgt tcgtctcgat
      901 cgtccataac ggcttccact ccatgcttgc acagttcgga atatatccgc tctgcggctt
      961 ccacttgcgc gctttccgaa gtattcagcg gcataaccac ggcctgatat ggagccaccg
     1021 acatcggcca tattatgcca tcgtcatcat gatgctcctc aatgacggag gcaaccgtgc
     1081 gcgtgattcc tattccatag catcccatta ttatcggatg ctcgactcca ccctcatcaa
     1141 ggtattttgc ccctaaagca gaactatatt tcgtcccaag cttaaacagg tggccaacct
     1201 ctacgccacg cgccccgcag agctccgctc cacatctagg acaggcatcg ccggcgacca
     1261 catttcttat atctgcaacg atatctgctg tatagtctac accatactct gcacctacca
     1321 ggtgcgcatc gacggcattg gccccaacca cggcatcctc aacaacagtt gcctcccagt
     1381 cggcaacgat cttgattcgc tccctaagcc caaccggccc tgtgaaccca acaggcgcac
     1441 cgcttacgcg ctcaatcact tcaggcgtgg caagctccag ctcacgacag cccaaaaccc
     1501 ttttgagttt ggcctcgtta agatcgcggt ccccgcgcac tatggcagct acgggtgtac
     1561 cgtctgccac gtagacaaga gttttcagca gcacccgagg ctccaccttg agatagcctg
     1621 taacctcctc gatagtccgc atacccggtg tcgatatctt ccttattcca gtacgaactt
     1681 tctcggtgtc agcaaccctc tctgccggct tagccgattc agcgcgctca acgttagccg
     1741 catatccaca cgcgtcgcag aaaaccaggg catcttcgcc aacctccgac acaaccataa
     1801 actcatggtt ctggcttccg ccaattgctc cggcgtcggc ttcaaccacc ctatagtcca
     1861 caccgcatct ggaaaacacc ctgcaatagg cctcatacat cttctcgtaa ctgcggtcaa
     1921 ggccctcatt atccctgtca aagctgtatg cgtccttcat tataaactcg cgcgaacgca
     1981 ttaccccaaa acgcggcctg atctcgtccc gatatttcgt ctggatctga tagaggatct
     2041 tcggcaactg tcggtacgac cttatttcat ccctcaccgt taccgtgatt atctcttcat
     2101 gcgtaggtcc aaggcacatt tcccttccgt ttcgatcctt aagacggaac atctcgctgc
     2161 cgtaatcatc ccatctgccc gtttcgcgcc atatgtcggc ggactgaatg gctggcatga
     2221 gaacctcctg cgctccctgc ctatccatct cctcacggac tatcttttct atcttgcgga
     2281 ccacacgaca accgagatga aggtaagaat aaaccccaga gacagtcttt cttaccatac
     2341 ccgcccttag catcagctga tggctcggaa tatccgcctc agacggcact tcccgcaacg
     2401 taggcgcaaa catctgcgac atcctcatcg taaaccaact cctctagcag ttaaccgtgt
     2461 gacttctcgt gccggttcgc cgccatcgat tcggcggcct ccacgagcgc ccctaccatc
     2521 tctgcctccc ggactttgcg aaccaccctc ccgcccgaga agatcaggcc taccccgttt
     2581 ccacatgcaa gcccaacatc tgcttcgctc gcctctccgg gcccgttcac ctcgcatccc
     2641 attatagcaa ctacgagtgg tgcgtccagg tgcgacaagc catccttgac ttccttcaca
     2701 atgcccgcaa gatcagttct acaccttcca catgtcggac acgacactat cgttggtccg
     2761 aatcgcctca tgccaaggct ggcaaggatc tcacggcccg cgattacttc atcccgggga
     2821 tctccagtga gactcaccct gatcgtatcc cccaattctt cagaaagaac catcccaata
     2881 ccgatagccg acttaatgag accgccgggg ggcgggcctg cttcagtgat tcccacatga
     2941 agaggatacg gcgccctacg cgcgatcaat cggtatgctt ccgtcgttct cttaacgtcc
     3001 gacgccttga gtgaaaggac aatcgattca aatccttccc tttcaaggag actgacatgg
     3061 cggagagcgg cctccaccat ggcctcggga gtgcggccat actttgacac caagtcggct
     3121 gggagcgaac ctgcattcac tcctatgcga atggggatac ccctatctgc ggccgcatga
     3181 gcgacctctc gaaccctgtc tgcacccccg atgttgccgg ggttgatgcg caatttatcg
     3241 acgcccgcct ctatgctggc caaagccaac ttgtagttga agtgtatgtc ggccaccaac
     3301 gggacgctag atgctttgcg tatgctccgc aaggagtcgg ctgcttcctc atctgggaca
     3361 gcaaccctaa caatctcgca tcccacagcg gcaagctgcc ggatctgctt gatggcagca
     3421 tcagcatcac gcgtatcagc tgtagtcatt gactggacag aaataggggc gccaccccct
     3481 atcggaacag atcctgccat tacctgtctt gtctcttcgc gagtcgccat ctgacaaaca
     3541 ccgcctgatc cggcagtaag ccgcatagtc tacatgcgtt gaaccataat cctcgagata
     3601 tcgctcacag tagcatacac gagcaagagc atgagcaacg ctagcccgat aaacctgaga
     3661 aaaccctcta cttgaggaga gagcggcttc cccctcacag cttcgatgat cagaaacaga
     3721 acccatccgc catcgagaaa aggaattggc atcagattga gcagccc
//
LOCUS       DAOSAI010000252         3722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15710_length_3722_cov_3.757567, whole genome shotgun sequence.
ACCESSION   DAOSAI010000252 DAOSAI010000000
VERSION     DAOSAI010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15710_length_3722_cov_3.757567"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1093..1659
                     /gene="lepB"
                     /locus_tag="PLM83_08315"
     CDS             1093..1659
                     /gene="lepB"
                     /locus_tag="PLM83_08315"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOK71444.1"
                     /translation="MQAILLAIVLAMVIMTFIGRSFVVEGASMEPTLHNRERIIVEKV
                     SYRFREPARGEIIVLKNPWRPDQSGMAAAMEAMRELVDFSGSMRPYIKRIVAVAGDRV
                     QIKNGVLYLNGKPIDEPYILERPWSDYGPVIVPEDHVFVLGDNRNNSDDSRGSVGFLR
                     VNRIVGRACVRYYPFDRMGVLKAPPVYE"
     gene            complement(1799..2269)
                     /gene="mscL"
                     /locus_tag="PLM83_08320"
     CDS             complement(1799..2269)
                     /gene="mscL"
                     /locus_tag="PLM83_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOK71445.1"
                     /translation="MLKEFQEFAVKGNVIDMAVGIIIGGAFGKIVSSLVNDIIMPPIG
                     LLVGKVDFSNLFIDLSGNGYATLAQANEAGAPTINYGLFINNVIDFLVVAFSLFLIVR
                     WINTLRATAAAAIENEEAPCASAPSTKECPYCLSEIPVKAIRCAHCTSDLSDGR"
     gene            complement(2415..2498)
                     /locus_tag="PLM83_08325"
     tRNA            complement(2415..2498)
                     /locus_tag="PLM83_08325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2463..2465),aa:Leu,seq:tag)
     gene            complement(2528..2604)
                     /locus_tag="PLM83_08330"
     tRNA            complement(2528..2604)
                     /locus_tag="PLM83_08330"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2568..2570),aa:Pro,seq:cgg)
     gene            3000..3557
                     /locus_tag="PLM83_08335"
     CDS             3000..3557
                     /locus_tag="PLM83_08335"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOK71446.1"
                     /translation="MPDLDAVAREAKECRRCKLGGMRKNAVPGEGNPHARIMFVGEGP
                     GADEDAQGRPFVGAAGRVLDKLIASMGLKREDVFIGNVVKCRPPGNRVPAPDEVEACM
                     PYLARQIELIDPDILVILGATALKALVDPNASITRMRGKWLERSGIRIMPTFHPAALL
                     RDTSKKALVWEDMKKVRACMKDFEA"
BASE COUNT          899 a          887 c         1105 g          831 t
ORIGIN      
        1 gtattcgatt gaattgctcg atatggctct gaacatgtaa ttctattgct aatgctgata
       61 gaatccgcgc gtgggggccg acgaggcccc tcgcgcgttt actgggttgc tcaccttatg
      121 gtaaccaaga ggaacatcgc ggcgcacagg atttgtctgg cggtctacag ttgcgtagcc
      181 gtattgggcc tagtcgtctg cattatgcac gtgccttggt aggtcggcag gaatgggtca
      241 gcagttgccg acaccgctag ggtgttgatg gtctttccga ctcaactgcg caaagcggag
      301 caagccggct ctgttgcagt cgcatgtgga taggcccgcg caaggcgggc ccaacgagcg
      361 atttgcccta gcacgggcgc atggtcccta ttacggcttt atggcccagg cgcatcgaat
      421 aggcaatggg agatgcaaca gaccaagaag caaacgaaga agacgtgtag ccaaaaccac
      481 catccgaaga agaaatgttc gccctttaca ggatctggca ttgcgcgctc acctccatga
      541 taacctatgg catcttatga tctaacgcga aatcgggaat tgtgaacaaa gcactgattt
      601 tcactggaga cactgcgcct atcaggcggt catgcctccg agactccgcg gcacttgcta
      661 tgtggtgcag taaacgatat tatcgtgtca gaagcccgca ggttgcggga acatagtcgg
      721 cgtcgttttc gttataacta tgtagaactt acaatgacgc cgatgcagaa ataaggtagg
      781 cgcaggttta tggataatgg catcgagaat gatggcgcag tcggcaaagg cgagtcggca
      841 gtcgcttccg cgcacgacgc cgtatcgagc ggcccatgtt cgggtgagcg tcctgattta
      901 tgtccggatg agggacgacc tgccgatgaa cgaattgaga atgtagacat tagaagcgaa
      961 tcggacgacg tagacatcga aggaatgatg gttggagcta tcgctgatga agcagcggca
     1021 gcagcagagg cagcgcgtct aagggaagca gccagcaagc gagaggctgc gtttcgcgag
     1081 gtaagagagt acgtgcaagc aatactcctt gcaatagtct tggctatggt tattatgaca
     1141 tttatcgggc gttccttcgt tgtggaagga gcttcaatgg agccaacgct ccacaaccgt
     1201 gaacgcataa tcgtggagaa ggtctcttat agattccgcg aacctgcgag aggtgaaatc
     1261 atcgtcttga agaatccttg gaggcctgac cagtccggaa tggccgctgc catggaagcc
     1321 atgcgggagc tcgtcgattt cagcggatcc atgaggccgt acatcaagag aatcgtggcc
     1381 gttgcaggcg acagggttca gatcaaaaat ggcgttctgt atcttaatgg caaacccatt
     1441 gacgagccgt atatacttga gcgcccgtgg tcggattatg ggcccgttat agtccctgaa
     1501 gatcacgttt ttgtcctcgg cgacaaccgc aataactccg atgacagtag gggaagcgtg
     1561 ggatttctcc gggtcaaccg tatagtcggc agagcctgcg ttcgctacta tccgtttgac
     1621 aggatgggtg tattgaaggc gccgccggtg tatgagtaat aatgttaggt gcatgacacc
     1681 gtagtccgag ataagcttga ggcatgcgaa tgacaataat gcccctgagc gcacgctccg
     1741 gggcgttatt gtttgcgcgg atggcaacct ctttcatacg gagggagacc ggccacgatt
     1801 atcgcccatc agaaaggtct gaagtgcagt gggcgcaccg aatagctttg actggtatct
     1861 ccgacagaca gtatgggcac tcttttgtgg aaggcgcaga tgcacatggc gcttcttcgt
     1921 tttcaatggc cgcagcggct gttgcccgga gagtgttaat ccatctcacg attagaaaga
     1981 gcgaaaaggc gactacaagg aaatcgatga cgttgtttat gaacagcccg tagttgatgg
     2041 tgggggcgcc ggcctcgttc gcctgcgcca aagtggcgta tccgttgcca gacaagtcaa
     2101 tgaagaggtt agagaaatcg acctttccta caagaaggcc tatgggcggc atgattatgt
     2161 catttaccag agacgatacg atttttccga aggcgcctcc gatgattatt cctacagcca
     2221 tgtcgattac attgcccttt accgcgaatt cttggaactc ttttagcatt agttaatcac
     2281 tccttcgccg ataaagcggc gttcccgtca caattggaaa actcggtttg gtcaagcaga
     2341 tacctgctgc aaatgaaaaa actgcaatag gccatcttaa ggctgatgca gtctagggga
     2401 gtttgtgttg aacatggtgc gagaggaggg atttgaaccc tcacggtttt acccgtcaga
     2461 tcctaagtct gatgcgtctg ccgttccgcc actctcgcac gtactgctgc gagccgaggc
     2521 ataagaatgg tcgaggtgcc gggatttgaa cccggggcct cttggtcccg aaccaagcgc
     2581 gctaccaaac tgcgctacac ctcgaccttg cgcccctcag agcacatcaa tagtatatgg
     2641 cattgccgca gtggtgtcaa accaaatcgc gccggaattt gttgatccgg aatgctgagt
     2701 gctcagcgca agggcaagca tggaagacct gcgaaacatg tgccataatt agtacaggat
     2761 agaaagaggg agatgtcatg gcagaggaga agcaactcac gctcttcgaa catggtgatg
     2821 cggaagattc catggtggaa cagatgaacc tatggagcgc cgaagcagag tcgaatcagt
     2881 cgactgcgca ggcaataccg tcctggtccg cagctgctca tataagcgct cctgtggctg
     2941 tacctttatc cgctcatcca aaggggctgt gggaaacgag tgttgtcagg gcccccgacc
     3001 tgccggatct tgatgcagta gcgcgcgagg caaaagagtg tagacgctgt aagctgggtg
     3061 gtatgcggaa gaatgcagtt cctggagaag ggaatccgca tgcgcgaata atgttcgttg
     3121 gtgagggacc tggcgccgac gaggatgcgc aagggaggcc gtttgtggga gctgcgggaa
     3181 gggttttgga caagctcatt gcatcgatgg gcttgaaacg cgaagacgtt ttcataggga
     3241 atgtcgtgaa atgccgtcct ccgggcaatc gtgtgccagc acctgatgaa gtggaggctt
     3301 gcatgcccta cctggcgcgt cagatcgagc tgatagatcc ggacatactc gtaatcttgg
     3361 gcgccacggc gctcaaagct ttggttgacc cgaacgcttc catcacgcga atgcgaggca
     3421 aatggcttga gcgaagcggg atccggataa tgcccacatt tcatccggcg gctttgcttc
     3481 gcgataccag caagaaagcc ttggtctggg aagacatgaa gaaggtcagg gcctgcatga
     3541 aagactttga agcgtagggg gaagaataac tgcgagcgca gactaatact cttccgatga
     3601 gggcaatgac cttgaggagg tgcggagatt atgagggcgg gtgctccgaa acgtgagagc
     3661 gaccgcgccc agatccccat ttggaagctg gttgcgctcg gttgcggcgt ttgtttcctt
     3721 gc
//
LOCUS       DAOSAI010000253         3719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15733_length_3719_cov_3.013100, whole genome shotgun sequence.
ACCESSION   DAOSAI010000253 DAOSAI010000000
VERSION     DAOSAI010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15733_length_3719_cov_3.013100"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLM83_08340"
     CDS             <1..238
                     /locus_tag="PLM83_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71447.1"
                     /translation="GDVRFRPEWRLQWTGVYDFFKGGLVRGDIGITRDLHCREVALTY
                     QHTSRRVWLEFRLKAFPGTPLGFGLGEEGILFQQ"
     gene            307..978
                     /locus_tag="PLM83_08345"
     CDS             307..978
                     /locus_tag="PLM83_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71448.1"
                     /translation="MDPRIVRIVSVAILLVLGCLYVYAFVLNKPADEDRSFDPDPDRA
                     SSDLTFGGVSIEGRENGERVWKISAANVDLRDGDGHVEMSEIGHGEIYRDGEPYVSFR
                     ACKGVFDTKTGQLSLSGGVDVFSGGRKLLRTDEVAWKPDDSRIVIPGQAEIITEQGVV
                     SAASLRVDVASDKIYASGDLTIREGFGGRDVVVKSGEICFDPAENSFEAWGGTEIEFE
                     IGDDD"
     gene            992..1672
                     /locus_tag="PLM83_08350"
     CDS             992..1672
                     /locus_tag="PLM83_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOK71449.1"
                     /translation="MKGQARKRAVSVVVLAVLLVSAAIVAEAAAKKVNLQVHGKVRYE
                     GSKGRTIVEGGLTIRYDDTVITATNAVIDNAAETAKLTGGVLLTQKDVVLNADEMDVD
                     LKNDTAVVKGNVRLEKREEQAEKDESGKAKVSVVKMTCNILEISTKTQDFIAQGSVLM
                     TKDKQKAQAEKANYDNAGKTLILQGSVAIEDEGNKTIKCDRAILYTDKELVEAEGKPV
                     EITFEIEE"
     gene            2261..>2955
                     /locus_tag="PLM83_08355"
     CDS             2261..>2955
                     /locus_tag="PLM83_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71450.1"
                     /translation="MYALVRGGFILDVAAVLLVTIILGSFVAAGIASAADDFFVHTVS
                     DVVGEYGEYDLIVHVRTESRREALRALRATLRAKLPGARVKESATVLSVSNFLVSLAK
                     KDEESVTALVQGVSGIPGFAGYTFLLEPKVTVSGVGSKTAPIVQDKLTRLDGVEFALR
                     DGGDIHLICESPESVKSVAQAAEAILGRYQVVDIRFAPSALASEGAFRGRLVQRVAAR
                     LAGGDVKGQVVRDV"
     assembly_gap    2956..2965
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3090..>3719
                     /locus_tag="PLM83_08360"
     CDS             3090..>3719
                     /locus_tag="PLM83_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71451.1"
                     /translation="MSVEDKPVGSLPAPEDMVVAVTDVDGDKARGIIVDGAKEPKDQS
                     SQVLSAYLLEDGVVSGRVGSATTSRPQEDLSRSLSEAEEALAQYDAGIADAMRAAKIG
                     EEALESYERAIDSLIYLQRALESIGVDGEADIMPIDDEAVSALLHLTEEALEAIEALR
                     STADVVVMFSGSYDSLLADLELWQARLKSFAQKLETIQSAATGAGQVAEM"
BASE COUNT          841 a          798 c         1239 g          831 t
ORIGIN      
        1 gggagatgtc aggttcagac ccgaatggcg tctgcagtgg actggtgtgt atgatttctt
       61 caagggcggt ctcgtgcgag gagacatagg gatcacccgc gatctgcact gcagggaagt
      121 ggctctcaca taccagcaca cgtcgagacg ggtgtggctc gagttcaggc tgaaggcatt
      181 cccaggcaca cctctcgggt ttggcctggg cgaagaagga atactctttc aacagtgagg
      241 ttgcgacatg aacactccgg tgtaaccttt ggccgtgctg agggcttgtc aggaggcgtg
      301 atgctgatgg acccgaggat tgtcaggata gtcagtgttg ccatattgct ggttcttggt
      361 tgcctgtacg tttacgcttt cgtgctcaac aagccggccg atgaagatcg aagttttgac
      421 ccggatccgg accgcgcctc cagtgatctc acgtttggcg gcgtcagtat cgagggtcgg
      481 gaaaacggcg agagggtgtg gaagatttcc gcggccaatg tggatctgcg agatggcgac
      541 ggccatgtgg agatgagcga gattgggcac ggcgagatat acagggatgg cgaaccctat
      601 gtctcattca gagcatgcaa aggcgttttt gatacaaaga cgggccagct gtcgctctca
      661 ggaggtgtcg atgttttctc aggagggcgg aagctgttga gaacggatga agtggcatgg
      721 aagcccgatg actctaggat tgtcattcca gggcaggcag agataattac cgaacagggg
      781 gtggtctctg ctgcttctct tcgggtggac gtggcctccg acaagattta tgccagcggt
      841 gacttgacga ttcgcgaggg gttcggcggt cgagacgttg tcgtaaaaag cggagagatt
      901 tgtttcgacc ctgcggagaa ctccttcgaa gcatggggcg ggactgagat tgagtttgag
      961 attggcgacg atgattgagg gggagagcgg aatgaagggg caggcgcgaa agagagcggt
     1021 gtctgttgtg gttttagcgg tgcttctggt ttcagcggcc attgttgcgg aggccgccgc
     1081 gaaaaaggtg aacctgcaag ttcatggcaa ggtacgttat gagggttcca agggcagaac
     1141 tattgtagag ggcggcctga ccatacggta tgacgacact gtgattactg cgacgaacgc
     1201 ggttatagac aatgcggctg agactgccaa actgactggc ggcgttcttt taacgcagaa
     1261 ggatgttgta ctcaatgctg atgagatgga tgtggatctc aagaatgata ctgctgtagt
     1321 gaagggaaat gtgaggctgg aaaaacgcga ggaacaggcg gaaaaggacg agtccggaaa
     1381 ggcgaaggtc tctgttgtga agatgacatg caacattctg gagatctcaa ccaagactca
     1441 ggattttatc gcccagggat cggtcttgat gaccaaggat aaacagaaag cccaggcgga
     1501 aaaggcgaat tacgataacg ccggcaaaac tctgattttg cagggttctg ttgccatcga
     1561 ggacgagggg aacaagacca taaaatgtga cagggcgatc ctctatactg ataaggaact
     1621 ggtggaagcg gaaggcaagc ctgtagagat cacctttgag atcgaggagt aacgcatgtg
     1681 gctggtcgtt gttcctgacc aagatcgatg ctgggttttt taggggacca aaagaagaat
     1741 ggaaggcgtg acgctaatcg tccctttcca tgcgaaggtg tagtcagtag tcgagtgaag
     1801 atcgggtatg aaggagggcc gcaaagcggc cctccttccc gcgttttcgc ccggcatatt
     1861 tgttgatcaa agaagtcctg gattgcccgt acccaaagat gtttacgtgt gaacatagcg
     1921 aaatcgaacc gggattggcc tgtcagctgg ataacctggg aggttggtag gactgctttt
     1981 agggagtgag gtttaaaagt agagccgtgt acgaggtccg tacgcacggt tctgtgagag
     2041 ggtggcgctc ccgattatcg ctatcgagct cgccaaaagc tgctgtagaa tccacaggtt
     2101 actgatgaac gcccctgaag cgggattcac tggagctttc agtgcccgta gttcagctgg
     2161 ctatgtcatt tgcggccaca tggaagacat tacatggcaa aagagaggtg caaagtgggc
     2221 acacatgcta taatgtcgag tgaatggggg gggtagctgc atgtacgccc ttgtgcgcgg
     2281 tgggttcata ctggacgttg ccgcggtgct tctagtcaca atcatactag gatcgtttgt
     2341 agcggcagga attgcgtccg ctgccgatga tttctttgtt catacagtat cagatgtagt
     2401 aggcgaatac ggcgaatacg atctgattgt ccacgtgagg actgagtcca gacgcgaagc
     2461 tttgcgcgcg ctcagggcga cactgcgcgc gaagctcccg ggggcccgag tcaaagagag
     2521 cgccaccgtc ttgtcggtgt cgaacttcct tgtgtcgttg gcgaaaaagg acgaggaatc
     2581 agtgacagct ttggtgcagg gggtgtccgg cattccgggg tttgccgggt atacgttctt
     2641 gctcgagcct aaggtgaccg tgagtggtgt cggttcgaag acagcgccga ttgttcagga
     2701 taagttgacg cggctggatg gagtggagtt tgctttgcgc gacggcggag atattcatct
     2761 catatgtgag tcgccggaga gtgtgaaatc tgtggcgcag gcggccgagg caatcctcgg
     2821 gcgctaccag gtggtcgata ttaggtttgc gccgagcgca ctggcttcgg aaggtgcttt
     2881 ccggggccga cttgtgcaac gcgtagctgc caggttggca ggcggggatg tgaagggcca
     2941 ggttgtgcgc gacgtnnnnn nnnnngaaag cattcctgga ggcctgggcg ccgcaagctt
     3001 ctatacgact ggacgaggga aaggtcaacc ctgtgcacac tggggcggat acggggtttt
     3061 caggcctgga tacaggagat tctgttgtct tgtctgtgga ggataagccc gttggatcgt
     3121 tgccggcgcc tgaagatatg gtggtggctg tgactgatgt cgacggagat aaggctcgcg
     3181 gaataatagt cgatggggca aaagagccca aagaccagtc gtcgcaggtt ctttcagcat
     3241 atctgctgga agacggtgtt gtttcaggcc gtgtgggaag cgctactaca agtcggcccc
     3301 aggaggacct gtcccgttca ctatcggagg cggaagaggc actggcacag tacgatgcag
     3361 ggattgcgga tgctatgagg gccgcgaaga ttggagagga agctttggaa tcatacgagc
     3421 gggccataga ctctctcatt tatctccaaa gggccttgga gagcattggc gtggatggcg
     3481 aagcggacat aatgcccatc gacgatgagg cggtgagcgc gctgctccat ctgactgaag
     3541 aggcgttgga ggcgatagaa gctctgcgct ccacggctga cgttgttgta atgttctcag
     3601 gaagttatga ctcgctcctt gccgatctcg agctgtggca ggccaggcta aagtcgttcg
     3661 ctcagaaact ggagacgata cagtcggctg caaccggcgc gggccaggtg gcggagatg
//
LOCUS       DAOSAI010000254         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15747_length_3717_cov_3.923812, whole genome shotgun sequence.
ACCESSION   DAOSAI010000254 DAOSAI010000000
VERSION     DAOSAI010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15747_length_3717_cov_3.923812"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..253
                     /locus_tag="PLM83_08365"
     CDS             62..253
                     /locus_tag="PLM83_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71452.1"
                     /translation="MKPQANNIDIRTGDILVEACTVAMAVSDASVGRAENAPRLPGER
                     AHDAEKTGKSLGTTKEVHR"
     gene            250..1575
                     /locus_tag="PLM83_08370"
     CDS             250..1575
                     /locus_tag="PLM83_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK71453.1"
                     /translation="MSGEATQTQQEKREDLRWNFRMSTLDGMFSNVAMGMVTPFLAVY
                     AMELGGSNNAVGLITAVPALVNTLMYLPAASIVEQSDSRLKIASKTHLLAKSVYLLMA
                     FVPFLPWPDLRSGVLIGLLGLQTIPTVITMVAWTALMGETFPREERAHLFALRSMYTS
                     LVTLVSSVLAGVLLDSIEYPINYVTLFLISYATSLVSFYFLSKMKEYPATPVKSVSTS
                     LGDRIKRPFTNPDYGAKFRVFTISAVLVHLGINLAVPAYPIMYVKDLGLSKSAIGSLS
                     LAGGLTAVLAYPMWGVVSRKAGDAAVYALSLLAFAAFPVLYGMSGSLVYLLIMKAAMG
                     IFDAGFAFTLFNLTLEYVDPEDSANGIAVFNVLINATGIIGPPIAAFVISRSGVMAAY
                     IVSSIVRTVGCVIFLKQFGVAETLGRLHRSLRLSLSRRRVTKQEKRGVL"
     gene            1579..1902
                     /locus_tag="PLM83_08375"
     CDS             1579..1902
                     /locus_tag="PLM83_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003384432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOK71454.1"
                     /translation="MKLVIVIVRDDYSEVLQDELADAGHRYTKLASTGGFLRKGNTTL
                     LVGVDDADVDSVLEIVRATCKTRQELITTQPMPMSPGLAVPPIEVIAGGATVFVVEVE
                     HFERI"
     gene            2511..2795
                     /locus_tag="PLM83_08380"
     CDS             2511..2795
                     /locus_tag="PLM83_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOK71455.1"
                     /translation="MVTISVCVGSSCHIKGAPEVIEKFQKLISDYDLYNLVELKGVFC
                     LERCTEGVTIDIDGNIYSAVNPDAAVDIFRERVLAPLGLTPKTGDDNDGQ"
     gene            2932..>3717
                     /locus_tag="PLM83_08385"
     CDS             2932..>3717
                     /locus_tag="PLM83_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOK71456.1"
                     /translation="MSLITTIRANCKDCYKCVRHCPMKAIKVVDGHAEVVDELCIGDG
                     TCVRICPQGAKQVRDSRGAVRDILRSGRKVALSVAPSFVSSFNDVDPGSFATAARGLG
                     FDIVTETAEAAYYVTISTMEALDRCEKPAIATSCPVIVNLVERYYPHLLGHLVPVASP
                     MVAHGRMLKNEMGPGSKVVFAGPCIAKKQEAEKEEAAGAVDAVLTFEELDSLFKEARI
                     VPAAQEPSEWNGRVPGVARLFPIEGGMMATAGIPNGGMGSADIC"
BASE COUNT          834 a          876 c         1103 g          904 t
ORIGIN      
        1 gcaaagtgga tagaagtgtc gtgtctgttg gcgttccttc ccgtgtaggg ggccaagtga
       61 ggtgaaacca caggcgaata atatagatat caggaccggg gatattttgg ttgaggcttg
      121 cactgttgcc atggcagtgt cagatgcttc ggtaggtcgg gcggagaatg cgccgaggct
      181 tccaggggaa cgcgcccacg acgctgagaa aacaggcaaa agtcttggta caacgaaaga
      241 ggtgcacagg tgagcggcga agcaacccaa acacagcagg agaagcggga agatctgcgg
      301 tggaattttc gaatgtcaac cctagatgga atgttctcca atgtagccat ggggatggtc
      361 accccatttc tagctgtcta tgccatggag cttggaggtt cgaacaatgc ggtggggttg
      421 atcacggcag tgcccgcact tgtaaacacg ctaatgtatc tccccgcagc ttcaatagtg
      481 gagcagagcg acagtaggct gaagatcgct agcaagacac acttgttagc aaaatcagta
      541 tatttgttga tggcttttgt gccttttctt ccatggccgg acctgcgttc cggcgtgctc
      601 attggtctgt tggggctcca gactattccg acggtaataa caatggtggc gtggacagcg
      661 ttgatggggg agacttttcc ccgcgaggaa cgcgctcacc ttttcgcgtt gaggagcatg
      721 tacacttctt tggttacact tgtctcctcg gtactggcgg gggtccttct ggactcgatt
      781 gaatacccga taaactacgt gacgcttttt ctcatatctt acgctacgtc tttggtgtca
      841 ttttacttcc tgtccaagat gaaggagtat cctgcaacac ctgttaagag cgtgtcaact
      901 tcgcttggcg acaggattaa gcggccattt actaatcctg actatggcgc caaattcaga
      961 gtattcacga taagtgccgt tctcgtgcac cttgggatca accttgcggt tccggcctac
     1021 cccatcatgt acgtgaagga tcttgggctt tcaaaaagcg ctatcggcag tctttcactg
     1081 gcgggtggtc ttacagctgt tctggcgtac cctatgtggg gagtagtatc gaggaaagcc
     1141 ggggacgccg cagtatacgc tttgtctctg ctcgcattcg ccgcctttcc agtgctttat
     1201 ggcatgtctg ggtctttggt ttatctcctc atcatgaagg cagcaatggg gatattcgat
     1261 gccggcttcg cttttacgct cttcaacctg acgctggagt atgtggaccc tgaggacagc
     1321 gccaacggga ttgcagtctt caacgtgctc atcaacgcca cggggattat agggcctccc
     1381 attgcggcat ttgttatatc ccgaagtggc gtgatggcgg cctacattgt gtcgtcgata
     1441 gtgcgaacag tgggatgcgt gatcttcctg aaacagtttg gcgtagcgga gactcttggg
     1501 aggctacaca gatcattaag gctatcgcta tcgcggaggc gcgtgaccaa acaagagaag
     1561 aggggggtgc tctaataggt gaagcttgtg atcgtaattg taagggatga ttactccgaa
     1621 gttttgcagg acgaacttgc tgatgctggc catcggtata ctaaacttgc ttccacagga
     1681 gggttcctgc ggaagggcaa cactacgttg cttgtgggcg tcgatgatgc cgacgtagac
     1741 tcggtgctgg agatcgtacg cgcaacatgc aagacacggc aggagttgat cacaacgcag
     1801 ccgatgccta tgtcgccagg ccttgcagtt cctccaatcg aggttatagc agggggagca
     1861 acagtttttg tggttgaagt cgagcacttc gagcgcatct agaagtctta ggatcaccca
     1921 tggggtaggc cgcggaactg atgtttccgt ggccttttcc attgtggcgg tggttctgtc
     1981 tctggggatt tccggccccc cgtcttcgtg cctcgggacg tgtctaaccg tatcggagtc
     2041 ttcagcgctc agacatccca ttctgtggcc gcagagcatt gatacagcat aatggataag
     2101 tgtgggacga aagccatgta aaggtcgagg tctcttgttt cacacgcata ttgcgctctt
     2161 acagtgtttg ctctaaagcc tgaggccttc ggtttgaccg ctcagttagc gcgcgggagt
     2221 ctgatatgga ggtttgggga ctgggatcgg cttctacctt gcaagcgccg atcctaggat
     2281 cgtgcaggaa cttattcgca gggcagttgg tttgctcttt tggacgttct taggcgagct
     2341 ttcatacaac tggccccaca tgcacgaatg catcaatctc aggcggatgc gggccgaaag
     2401 cggccaaaat cagctgggcg caaattgtct tgtacgaagt gttgtatatt gtggcggaaa
     2461 ctgttattat tgaattgtga aagactgcac aaagttggga cggcgataaa atggtcacca
     2521 tctcggtatg cgttggaagc tcgtgtcata tcaagggtgc tcctgaagtc atcgagaagt
     2581 tccagaagct catatccgac tacgatcttt acaacctggt cgagctcaaa ggcgtgtttt
     2641 gcctggaacg ttgcaccgaa ggcgtaacca tcgatatcga tggcaatatc tattccgcgg
     2701 tgaatccaga tgctgccgtg gacatcttca gagagcgcgt gcttgccccc cttggtctga
     2761 ccccaaaaac tggagatgat aatgacggtc aatgaggcct ggtgagccag gacaacgcgt
     2821 cttggtgccg ggccttagct gcacttggag attgaaaagg gctcaagggg gcaaaaacct
     2881 gaagaatcgc gagtgcgact cggatgtaga tgatcaaggg ggctgcaaca attgagtctg
     2941 atcacgacta taagggccaa ttgcaaggat tgttataagt gcgtgcgtca ctgtcctatg
     3001 aaggccataa aggtggtgga cggacacgct gaagtcgttg atgagctgtg tataggcgac
     3061 ggaacatgtg tgcgaatatg ccctcaggga gccaagcaag tccgggattc ccgcggggct
     3121 gtccgcgata tcctcaggtc gggcaggaag gttgctcttt ccgtggcgcc atctttcgtg
     3181 tcgagcttca acgatgtgga ccctggatca tttgccaccg cagccagagg acttgggttc
     3241 gacatagtca cggaaacggc tgaagcggcc tactatgtca cgatatccac catggaggct
     3301 ttggacaggt gcgaaaaacc tgccatagca accagttgtc cggtcattgt aaacctggtc
     3361 gaacggtatt atccgcatct actgggccat ttggtccctg tggcttcacc catggttgcc
     3421 catggaagaa tgctcaagaa tgagatgggg ccgggatcca aagtcgtatt tgcagggcct
     3481 tgcatagcta agaagcagga ggcggagaag gaagaggccg caggcgctgt cgacgcagta
     3541 ttgaccttcg aggaactgga cagtctgttt aaagaggctc gcatcgtccc cgcagctcag
     3601 gagccaagcg agtggaacgg gcgcgtgccg ggcgtggcga ggctgttccc aattgagggc
     3661 ggcatgatgg cgacggcggg aatcccaaac ggtggcatgg gaagcgcaga catctgc
//
LOCUS       DAOSAI010000255         3714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15766_length_3714_cov_3.895600, whole genome shotgun sequence.
ACCESSION   DAOSAI010000255 DAOSAI010000000
VERSION     DAOSAI010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15766_length_3714_cov_3.895600"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /gene="thrS"
                     /locus_tag="PLM83_08390"
     CDS             <1..1757
                     /gene="thrS"
                     /locus_tag="PLM83_08390"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOK71457.1"
                     /translation="HVTILDATSPEGLNTMRHSAAHVMAQAVKRLFPGVKLAIGPTID
                     DGFYYDFDIDHAFTSEDLEKIEAEMAKIIAEDLPIVRQEVSWVEAMDVLKSGDEVYKM
                     ELLNELGDGEVSFYRQGEFCDMCRGPHLPRTGMIGAFKLLSVAGAYWRGDENRPMLSR
                     IYGTAFPTRKELDEHLRLLEEAAKRDHRKLGKELDIFSVHEEAGAGLIFYHPHGAAIR
                     HSIEDFWMREHIARGYKPVITPHIAESTLWTISGHNDYYRENMYYLSIDEKEYVLKPM
                     NCPGHILIYKTATRSYRDMPIRYCELGTVYRYERSGALHGLLRVRGFTQDDAHIFCTP
                     EQLRDEIVGVIDLTQFMLKSFGFSEYEFFLSTRPEKSVGSDENWERATAALRDALEGH
                     GLKYTVDPGEGVFYGPKIDTKLKDSLGRLWQGPTIQVDFNLPERFDVNYIGEDGEKHR
                     AIMIHRTVLGSMERFMGCLIEQYAGALPVWLSPVQAKVLSIGDRHIDYAMGIARNLSE
                     RGFRVDTDVRDEKIGYKIREAQLEKTPYMLIVGDREMESGQVAVRARRGGDLGAMTLN
                     QFADRLRAEVDSRTCEDV"
     gene            2003..2524
                     /gene="infC"
                     /locus_tag="PLM83_08395"
     CDS             2003..2524
                     /gene="infC"
                     /locus_tag="PLM83_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOK71458.1"
                     /translation="MSRELFVNEAIRAREVRVVDENGEQLGIMPVREAIKLAADRDLD
                     LVEVAPTARPPVCRIMDYGRYKYEQSKREREARKKQHVVDIKEVRMTPKIEEHDFSVK
                     TKSAERFLRDGDKVKVTIRFRGREIVHSDLARQLLMEMAESLSSVGSVERLPKVEGRN
                     MVMILSPKQEQEG"
     gene            2567..2764
                     /gene="rpmI"
                     /locus_tag="PLM83_08400"
     CDS             2567..2764
                     /gene="rpmI"
                     /locus_tag="PLM83_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOK71459.1"
                     /translation="MPKMKTNRASAKRYYVTGSGKIMRNKAGRRHKLAAKTPKRKRNM
                     RKDVVLSKPDQKVARELIPYK"
     gene            2814..3176
                     /gene="rplT"
                     /locus_tag="PLM83_08405"
     CDS             2814..3176
                     /gene="rplT"
                     /locus_tag="PLM83_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOK71460.1"
                     /translation="MARVKRGTNARRRHNQTLKLAKGYTGVNSVSYKRANQAVMHAMD
                     YSYAHRRTRKRDFRQLWIARINAAARMNGMSYSRFISGLKKAGVDINRKMLADLAVND
                     GAAFEQLVAVAREQEAAN"
     gene            3296..>3714
                     /locus_tag="PLM83_08410"
     CDS             3296..>3714
                     /locus_tag="PLM83_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOK71461.1"
                     /translation="MEDALAAKVPLDTVYIAEDVDMGNESTSGRILSGARRNGARIVI
                     VSTSVAESMADTRTPQGVFALAKWSPASGCELLASAGGNGMPSFGVYLDGVSDPGNVG
                     TIIRTAAALGALGVVAGPRCAGFTNPKVIRASMGSIFR"
BASE COUNT          913 a          843 c         1126 g          832 t
ORIGIN      
        1 gccatgtgac tatactggac gcaacatcgc ccgaggggct taacacaatg cgccattccg
       61 cggcgcatgt aatggcacag gctgtgaaga gactgtttcc cggagtaaag ctcgcaatag
      121 ggccgacgat cgatgatggg ttctattacg atttcgacat cgaccatgcc tttacttccg
      181 aggatcttga gaaaatcgaa gcggaaatgg cgaagatcat cgccgaggat ctcccaatag
      241 tccgtcaaga ggtgtcatgg gtcgaggcca tggacgtgtt gaagagtggc gacgaggtct
      301 acaagatgga gctcctcaat gaactcggtg atggtgaggt cagcttctat cgacagggcg
      361 agttttgcga catgtgtcgt gggcctcatc tgccgagaac gggaatgatc ggcgccttca
      421 agcttctcag cgtggctggg gcgtactggc gaggcgatga gaacaggccc atgctctcaa
      481 gaatatacgg aaccgctttc cccacccgga aagagctcga cgagcatctg cgccttctgg
      541 aggaggctgc caagagggat catagaaagc tcggcaagga gctcgacata ttcagcgttc
      601 acgaggaggc aggcgctggg cttatcttct atcaccctca tggcgcagcc atccgccact
      661 ctattgagga cttttggatg cgtgaacaca tagcgcgggg gtataagcct gttatcactc
      721 cgcacattgc agaatcgacg ctatggacca tatcagggca caatgactat taccgggaga
      781 acatgtatta cctgtccata gacgagaagg agtatgttct gaagcccatg aattgtcccg
      841 ggcatatact gatatacaag actgctacga gaagctatcg ggatatgccg atacgctact
      901 gcgaactggg gactgtgtac agatatgaac gttctggggc tcttcacggg ttgctcaggg
      961 tgaggggatt cactcaggat gacgctcaca tcttttgcac ccctgagcag ctgcgggatg
     1021 aaattgtagg ggtgattgat cttacccagt tcatgctgaa gagctttgga ttttcggaat
     1081 acgagttttt cctgtcaaca aggccagaga agtcggtggg atcggacgaa aactgggagc
     1141 gtgcgacagc ggctctccgg gatgcattag aaggccacgg gcttaagtat accgtagatc
     1201 caggtgaggg cgtgttttac ggcccaaaga tcgataccaa gctcaaggat tctctcggcc
     1261 gtctctggca ggggccaaca attcaggtgg atttcaactt gcccgaaagg ttcgatgtaa
     1321 actacatcgg agaggatgga gagaaacaca gggctatcat gatacacaga accgtccttg
     1381 gtagcatgga gcggtttatg gggtgtttga ttgagcagta tgcaggcgcc ttgcctgtat
     1441 ggctctctcc agttcaggca aaagttctct ccatcggcga tcgacatatc gactatgcca
     1501 tgggaattgc caggaacttg tcggaacgag ggtttagggt tgacacggat gtccgagatg
     1561 agaaaatcgg atacaagata cgcgaagccc aacttgaaaa gactccgtac atgctcatcg
     1621 taggcgacag ggaaatggaa tccggccagg ttgcagtgag ggcgcgccga ggcggtgatc
     1681 tgggtgccat gactttgaat cagttcgccg acaggcttcg cgcagaagtt gactctcgca
     1741 cgtgcgagga tgtttgacgc agggccatgc ctgtgttata ctcggtcttg cgaatctggg
     1801 atacatgttt gaagtagaag ccatccgctt ctcaccgatc ggcgttgctc aacgcttcag
     1861 ccaggtcggt tcacttgact atatgcggac cggcaaattt gccgagcgcc gtatgctctg
     1921 tcattgtggg cgggtggatc acccgcttgt tctttatttt gggggcattc aaacactgaa
     1981 atttttatgg gggtgacaaa ctatcagtag ggagcttttc gtcaacgagg cgatcagggc
     2041 cagggaagtc cgcgtcgtgg atgaaaacgg tgaacaactg ggcataatgc ctgtcaggga
     2101 agcgatcaaa cttgccgcgg atagggacct ggacctcgtg gaggttgcac ctacagcacg
     2161 accgcccgtc tgccggatca tggattatgg cagatacaag tatgaacaga gcaaacgcga
     2221 gcgggaggca aggaagaagc agcatgttgt cgacataaag gaagtccgga tgactccgaa
     2281 gatcgaagag cacgacttca gtgtcaagac taagagtgcg gaacggttcc ttcgtgacgg
     2341 cgacaaggtt aaggttacta tacggttcag aggacgagaa attgtccata gcgacctcgc
     2401 caggcagctt ttgatggaga tggcggagag cctttcaagt gtaggttctg tagagcgctt
     2461 gccaaaggtt gaaggcagaa acatggttat gattctttcc cctaagcaag agcaagaggg
     2521 gtaggtctgt ttttttgcgt ggattcagtt gaggagggca atggttatgc ctaagatgaa
     2581 aactaacaga gcatcggcga agagatatta tgtgaccgga tccggtaaga tcatgcggaa
     2641 caaggccggg cgtaggcaca agcttgctgc caaaaccccc aagaggaagc gaaatatgcg
     2701 gaaggacgtt gttctttcga aaccggatca gaaggtcgcg cgcgaactga tcccgtataa
     2761 gtagcttttc tgataggttg ttttgcatga tggccatagg gggttgattt acgatggcga
     2821 gggtaaagcg tggcacgaac gcgcggcgca ggcacaacca aactttgaaa ctcgcaaagg
     2881 gttacactgg agttaacagc gtttcatata aacgggctaa ccaggcagta atgcatgcca
     2941 tggactattc atatgcccac aggcgcacgc gtaagcgcga tttcaggcag ctttggattg
     3001 cgcggatcaa tgctgcggcc cgcatgaatg gcatgtcata tagccggttc atttctggac
     3061 tgaaaaaggc tggagttgac attaatcgca agatgttggc ggacctggcc gttaatgatg
     3121 gcgctgcttt tgaacagctc gtggctgtgg ctagggaaca ggaagccgca aactagtgtg
     3181 agcggtgagt agattggacg taatcaggag ccaaaagaac cccgctgtcc gcatgtacaa
     3241 acaatgcgtg actcggggtt tatcgcatct tgtcccactt gagggtgtaa ggctggtgga
     3301 ggatgcgctg gcggccaaag tgccgctaga cacagtctac atagccgaag atgtcgatat
     3361 gggcaatgaa tcgaccagtg gtcgaatact gtcgggcgct cgccgaaatg gtgcacgaat
     3421 tgtcattgta tccacgtcag ttgcggaaag tatggccgat acgcgcacgc ctcagggggt
     3481 gttcgcgctg gcgaagtggt cgccggcctc gggatgtgaa ttgctcgcca gcgctggtgg
     3541 aaatggaatg ccgtctttcg gggtctattt ggatggagtc tcggatccgg ggaatgtggg
     3601 aaccatcata cgcactgcgg cagccctagg agcgctggga gtggttgccg gtccacgatg
     3661 tgcaggcttt accaacccga aggtcataag ggcttccatg ggttctatct ttcg
//
LOCUS       DAOSAI010000256         3708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15797_length_3708_cov_3.187243, whole genome shotgun sequence.
ACCESSION   DAOSAI010000256 DAOSAI010000000
VERSION     DAOSAI010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15797_length_3708_cov_3.187243"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLM83_08415"
     CDS             complement(<1..938)
                     /locus_tag="PLM83_08415"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOK71462.1"
                     /translation="MNRYLMSFLFTIASSFLLTPQIRAFALKRGIVDRPRGRHVHKAP
                     VPMLGGVAIYLSFAAACLIFTKVNKNAIGLLLGGGLIVAFGVWDDICEIKPRTKLCGQ
                     IAAAVVLVLFGVKIEFVTNPLGGMIYLGFMSVPLTIIWLVAFMNVVNFIDGLDGLAAG
                     VSAIGALAMAFAAFQTKQGQLVIMSMALAGSALGFLYYNFNPASIFMGDAGAMFLGFA
                     IAGISAMGALKTPATLALAVPVLVMGVAILDTAFAIFRRVRKGAPVSVGDKDHVHHRL
                     LAMGMTQREAVLTLYAVSGLLAAAACGIVVVNPRVGL"
     gene            complement(1062..1520)
                     /locus_tag="PLM83_08420"
     CDS             complement(1062..1520)
                     /locus_tag="PLM83_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOK71463.1"
                     /translation="MDGAGRERPSWDEYFMEIAKVAAKRSTCLRRQVGAVAVRDKQIL
                     ATGYNGAPVGLAHCSEVGCVRAKLGIPSGERHELCRGLHAEQNLIIQAAVHGVAISGA
                     TVYCTFHPCVVCSKMLINAGIARIVFAGEYPDRLADEMLQEAGVELTRIP"
     gene            complement(1549..2178)
                     /gene="upp"
                     /locus_tag="PLM83_08425"
     CDS             complement(1549..2178)
                     /gene="upp"
                     /locus_tag="PLM83_08425"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOK71464.1"
                     /translation="MGEVHVIDHPLIQHKLTLIRDKNTGPKDFRDLVGEVAMLMAYEV
                     TRDMQLESIEVETPIAHTSGKQLAGKKVAVVAILRAGLGMVDGVLKLIPAAKVGHIGL
                     YRDPDTLRPVEYYCKLPYDVPERELIMLDPMLATGGSACAAIQFAKDRGVTNIKLVNL
                     IAAPEGIRLVQQAHPDVEIYVAAVDERLDSHGYIVPGLGDAGDRLFGTK"
     gene            complement(2202..2633)
                     /gene="rpiB"
                     /locus_tag="PLM83_08430"
     CDS             complement(2202..2633)
                     /gene="rpiB"
                     /locus_tag="PLM83_08430"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOK71465.1"
                     /translation="MKVAIGSDHAGYDAKEEVLKKLSDEGHDVVDFGTYSHESVDYPD
                     IAHAVAEAVASGECERGILLCGTGIGMSIAANKVRGVRAALCHDDFTAKMAREHNDAN
                     ILAVGSRTTAMHTIMRMVDIFLSTEFAGGRHADRVSKIEKA"
     gene            complement(2630..3088)
                     /locus_tag="PLM83_08435"
     CDS             complement(2630..3088)
                     /locus_tag="PLM83_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOK71466.1"
                     /translation="MPRKILLVCGGNTCRSPMAEAIMRKVIESEFPQMAGEIVVESAG
                     VAAVEGDTASENALTAAAKIGLDLSDHRARALTAKLISEADIILAMEGKHIASILAMD
                     PSAAGRVHTLADADIADPFGSSLEIYERTRDEIEAAVRRSLDVINRGDAG"
     gene            complement(3101..>3708)
                     /locus_tag="PLM83_08440"
     CDS             complement(3101..>3708)
                     /locus_tag="PLM83_08440"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HOK71467.1"
                     /translation="GSADIGVESTVIDMTCSPPMILRPGGITLEEIREVIPETVVLTG
                     GEGLDGPAKSPGLRHRHYSPEAQMWLVIGEWDAQVDEIKSRAAAGVRLGRRVGLLVST
                     ETAAKLCTWIRDCGGEGPAGEIIVAEIGSRNDASQVASGLFDGMRRLDAAEVDMMLAE
                     SYDARGMGLAIMNRLMRAAGGRIIRVQGEAGNDLISEKSIE"
BASE COUNT          860 a         1185 c          835 g          828 t
ORIGIN      
        1 aggccaactc gagggttcac aaccactatc ccacacgctg cagctgcaag gagcccgcta
       61 actgcgtata gcgtgagaac agcttcacgc tgggtcattc ccatggccaa caacctatgg
      121 tgaacgtgat ccttgtcgcc gacagaaact ggcgcccctt tgcgaacacg cctgaagatg
      181 gcaaaagccg tatcaagtat cgcaaccccc atcaccagca ccgggactgc caacgccagt
      241 gttgccgggg tcttaagcgc gcccatggca gatatgccgg ctatggcaaa accgagaaac
      301 attgccccag catcccccat gaatatcgaa gcggggttga agttgtagta taggaaaccc
      361 aatgcactcc ccgctaacgc catagacatg ataacgagct gaccctgctt agtctggaat
      421 gcggcaaaag ccattgcaag ggcgcctata gccgacactc cagcagctaa accatcgaga
      481 ccatcaatga agttgaccac attcatgaac gccacaagcc atatgattgt aagcggcacc
      541 gacatgaacc cgagatagat catgccgcca agcgggttag tcacgaattc gatttttacc
      601 ccaaaaagaa ccaacacgac agcagctgca atttgcccac aaagcttggt tcgtggcttg
      661 atctcgcaaa tatcgtccca caccccaaaa gccactatga gaccgccccc aagaagcaat
      721 ccaatggcat ttttgttgac ctttgtaaat ataaggcatg ctgcagcgaa tgacagatag
      781 attgccactc cccccagcat tggcacaggc gccttgtgga catgtctgcc ccttggacga
      841 tcaactatgc ctcgtttcaa ggcaaatgcc cgtatctggg gagtgagtag aaatgatgat
      901 gcaattgtga acaggaaaga catcaaatac ctgttcaagt tgtcgcactc ccatcggttc
      961 ggtataaaac aatcatgacg ccgaatccag gctagatccg tgtcgatcac tgcggatgga
     1021 tcttgcccca gtttcctcgc agaagtctgt acacacggac gtcaaggaat ccgagtcagt
     1081 tctacgccgg cttcctgcag catctcgtct gctaatcgat ccggatattc ccccgcaaat
     1141 acgattctcg cgatccccgc attgatcaac attttagaac atacaacgca cggatggaac
     1201 gtacagtaaa ctgtagctcc tgatattgcc acaccgtgca cagctgcctg gattatcagg
     1261 ttctgttcgg catgaagccc acggcagagc tcgtgccgct caccagaagg gataccgagt
     1321 ttcgccctca cacacccgac ttcactgcaa tgggcaagtc cgacaggagc tccattatac
     1381 cccgtcgcca ggatttgctt gtcccttact gctactgcac caacttgccg tctaagacac
     1441 gtagatcttt ttgcagccac ctttgctatc tccatgaaat actcgtccca tgaaggcctc
     1501 tcccggcctg ctccatccat agccatacct tctctgcgca gacgcgcgct atttggttcc
     1561 gaacagcctg tctccggcgt cgcccagacc aggcactatg tagccatggg aatcgagtcg
     1621 ttcatctacg gccgcaacat agatctcgac atcaggatgg gcctgctgga caaggcgaat
     1681 gccctccggc gccgcaatca agtttaccag cttgatgttg gttacccctc tgtccttggc
     1741 aaactgaatt gccgcacagg cagagcctcc tgtagcgagc atcggatcga gcattatcaa
     1801 ttcgcgttca ggcacgtcat atggaagctt gcagtagtat tctaccggcc ttagcgtgtc
     1861 aggatcacgg tagagcccga tgtgtcccac ctttgcagca ggaatcaatt tcaggacccc
     1921 gtctaccatg ccaagaccag ccctgagaat ggcgacaaca gccactttct tcccggccaa
     1981 ttgcttgcct gacgtgtgcg ctatcggggt ctcaacctcg atagactcca gctgcatgtc
     2041 gcgggttacc tcgtatgcca tgagcatggc aacctcgccc accagatccc tgaagtcctt
     2101 cggtccggtg ttcttgtctc taatgagagt taacttgtgc tgtatcaatg gatgatcaat
     2161 tacgtggact tctcccaatc agtacacccc cgatagcctt actaggcctt ttcaatcttg
     2221 cttaccctgt cagcgtgcct tccccctgca aattccgtag acagaaatat gtctaccatt
     2281 cgcattatcg tgtgcatggc agtagtgcgg gagccaacag ccagtatgtt cgcatcattg
     2341 tgctctctgg ccatcttggc cgtgaagtcg tcatggcaga gcgcagccct cactccgcgc
     2401 accttgttcg ccgcaatcga catccctatc ccggtgccgc acaggagtat gcccctctca
     2461 cactcgccac tggcgactgc ctcagccact gcgtgggcaa tatcaggata gtccacactt
     2521 tcgtgggaat atgttccgaa gtccacgaca tcgtggcctt cgtctgaaag cttcttcaaa
     2581 acctcttcct ttgcatcgta accggcatgg tccgatccaa tagccacttt caaccagcgt
     2641 cacctcgatt gatcacgtcc aaggatcgcc tcacagccgc ctctatctcg tccctggtcc
     2701 gttcatatat ctcaagtgaa ctcccaaaag ggtccgcaat atctgcatca gcaagagtat
     2761 gcacccggcc agctgctgat ggatccatcg ccagaatgct tgcgatgtgc ttaccctcca
     2821 tcgcaaggat tatgtcggcc tccgagatca gcttcgctgt gagcgctctg gcacgatggt
     2881 ctgacaggtc aaggccgatc tttgcggccg ccgtcaacgc attttccgac gctgtatcgc
     2941 cctctacagc agcaacgccc gccgattcga ccacgatctc gcccgccatt tgaggaaatt
     3001 ctgactcgat aacctttcgc atgatggcct cagccatcgg gctgcggcac gtgttcccgc
     3061 cgcagacaag cagaatctta cggggcactg cgttcaccgc ctattcgatg gatttctccg
     3121 aaatcagatc atttcctgcc tcgccctgca ctcttatgat ccgcccgcca gccgccctca
     3181 taagcctgtt cattatcgca agccccatgc ctcgagcatc atacgattca gccaacatca
     3241 tatcgacctc tgccgcatcc agccttctca ttccatcgaa gagccccgat gcaacttgag
     3301 aggcatcatt cctcgacccg atctcagcta cgatgatctc acccgccggc ccttccccac
     3361 cacaatcgcg aatccacgtg caaagtttcg cagccgtctc cgtgctcacg aggagcccta
     3421 cacgccgccc taggcgcacc ccagcggctg ctcggctctt gatctcatcc acctgcgcat
     3481 cccactcgcc tataaccagc cacatctgtg cctccggcga gtagtgccta tgcctcaggc
     3541 ctggagactt tgcaggccca tccaagcctt caccgcccgt caacactaca gtttcgggga
     3601 tgacctcacg tatctcctca agggtgatcc ctcccggccg cagtatcatc ggaggcgaac
     3661 aagtcatatc gatgacagtc gattccacac caatgtcagc agaccccc
//
LOCUS       DAOSAI010000257         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15833_length_3701_cov_3.442951, whole genome shotgun sequence.
ACCESSION   DAOSAI010000257 DAOSAI010000000
VERSION     DAOSAI010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15833_length_3701_cov_3.442951"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3047)
                     /gene="nuoF"
                     /locus_tag="PLM83_08445"
     CDS             complement(<1..3047)
                     /gene="nuoF"
                     /locus_tag="PLM83_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOK71468.1"
                     /translation="MDLVRAHVLVCGGAACVSSGCAEVREALEAEIAAKGIGREIKVV
                     VTGCMGPCDLGPIIVVYPEGVLYCNCTPDDAREIVDEHLVKGRIVSRLLYEEPMTSEK
                     VETQEGMTFFSRQERIALRNTGVIDPLEIDEYIARDGYAALGKALTEMTPDDVVDVIK
                     RSGLRGRGGAGFPTGTKWLLTAKAPGSPKYVVCNADEGDPGAFMDRSVLEGDPHAVLE
                     AMTIAGYAVGANQGYIYVRAEYPLAVEHLQHAIGQAREYGLLGKDILGSGFDFDIDLR
                     VGAGAFVCGEETALLASIESRRGEPRPRPPYPAQSGLWGQPTLINNVETYANIAPIIL
                     KGPEWFSSIGTEKSKGTKVFALAGKINNTGLVEVPMGTPLGDIIFDIGGGIPNGKKFK
                     AAQTGGPSGGCIPAQYLNTPIDYESLKELGTMMGSGGLIVMDEDTCMVDLAKFFMQFI
                     QDESCGKCAPCRIGTKRMLEILDRITKGQGREGDVELLMELGEQIMATAMCGLGQSAP
                     NPVLSTIRYFRDEYDAHIKDHKCPASSCAALFYSPCQNSCPADVDVPRYIALIKERKF
                     AEAAALIRSKNPFPAICGRVCDHPCESKCRRATFDEPVAIKSLKRFAADWEKKHGVSP
                     VAEHKPPTGKRVAIVGAGPAGLSAAYYLALAGHKVTVFEALPVAGGMLAVGIPEYRLP
                     KDILEWEIEVIKKAGVEIRTNTRIGEDIKLDELSSEYDALFIAVGAHNEVKMDVPGED
                     FEGVMPAVAFLRDVALGKPVQVGKKVAVVGGGNAAIDAARTARRMGAEEVHILYRRLR
                     ADMPADAGEIEAAEEEGIHIHYLTAPTRIIGRDGKVAQIECVRMKLGEFDKSGRRRPI
                     PVDASRTIVDIDTVIPAIGQAVDTSFIMPLSDTGENLFTTKSGLLITDPDSLETNVPG
                     IFAGGDCVTGPWSVVAAIGAGREAAYSIDRFLGGDAWIGAHAEIERCLVGEPIEEECK
                     RCPMPELPVSERARNFKEVELGFTEDMAVAEAARCFQCDVREP"
     gene            complement(3086..3580)
                     /locus_tag="PLM83_08450"
     CDS             complement(3086..3580)
                     /locus_tag="PLM83_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOK71469.1"
                     /translation="MGCCESKCVGDITPEQWAQVDGIIAKYKGQRGILIPCLHEIQEV
                     VGYLPLEVQERVAAGLNVPLADVYSVVTFYALFSTKPKGKHQVGVCLGTACYVRGGDK
                     ILEKVEQALGIKAGDTTDDRMFSIQVMRCIGACGLGPVMTVDDDIYARIKPDRVAEVL
                     AKYS"
BASE COUNT          788 a         1167 c          886 g          860 t
ORIGIN      
        1 tacggctcgc ggacgtcaca ctggaagcag cgagcagctt ccgccaccgc catgtcctct
       61 gtgaatccaa gttcgacttc cttgaagttc cttgcgcgct cagagacggg caattccggc
      121 atcggacaac gcttgcactc ctcctcgatc ggttcgccga ctaagcagcg ctcgatttcg
      181 gcatgagctc caatccaggc atcgcccccg agaaacctgt ctatgctgta tgctgcctcg
      241 cgtcctgcgc caatcgcagc cacgacactc catggtcccg tcacgcaatc cccgcccgca
      301 aagatcccgg gaacattggt ctccaacgaa tcagggtccg tgatgagcaa gcctgatttc
      361 gtcgtaaaga gattttctcc cgtatcagac agcggcatta tgaacgatgt gtcgactgcc
      421 tgcccaatag cgggtatgac ggtatcgatg tcgactatcg tgcgcgatgc atccacagga
      481 attgggcgcc ttctacccga cttgtcgaac tcgccaagct tcatcctgac gcattcgatc
      541 tgagccacct tcccgtcccg gccaatgatc ctggtggggg cggttaggta gtggatgtgg
      601 atgccctctt cctcggcagc ctcaatctcg cctgcatcag caggcatatc cgcccggagt
      661 ctgcggtaaa ggatgtgaac ctcttcggcg cccattcgcc gcgccgtgcg agccgcatcg
      721 atcgcagcgt ttccgccgcc aacgaccgcc accttctttc ccacctgaac aggcttgccg
      781 agcgccacat ctcggaggaa agccactgca ggcatgactc cttcgaaatc ctccccggga
      841 acgtccatct tcacttcatt atgagcacca accgctatga ataaggcatc gtattccgat
      901 gacaactcgt caagttttat atcttctccg atccgcgtgt ttgttcgtat ctccacgcca
      961 gccttcttga ttacctcgat ttcccattcc aagatatcct tggggagcct gtactcgggg
     1021 atccctacag ccagcatgcc gccggcgaca ggcaaagcct cgaacacagt aaccttgtgc
     1081 ccggctagtg cgagatagta ggcggcgcta aggcccgcgg gaccggcacc cactattgca
     1141 acgcgtttcc ctgtcggcgg cttgtgctcc gccacaggtg acacgccatg tttcttctcc
     1201 caatccgcag caaatctctt gagagacttg atcgctacag gctcgtcgaa tgtagcacgt
     1261 ctgcacttgg attcgcacgg gtggtcgcag acccgcccgc agatagcggg gaacgggttc
     1321 ttggaacgga tcagcgcagc cgcttcagcg aactttcgtt cctttatcag agcaatgtag
     1381 cgaggcacat ctacatcagc agggcatgag ttctggcacg gagagtagaa gagtgccgcg
     1441 cacgatgacg ccgggcactt gtggtctttg atgtgagcgt catactcatc gcggaagtag
     1501 cgaattgtgg acaaaaccgg gttgggagcg ctctgtccca ggccacacat ggcagtggcc
     1561 atgatctgct ctcccagttc catcaggagc tcgacgtcgc cttccctacc ctgtccctta
     1621 gttatgcgat cgaggatctc aagcatccgc ttggtgccta tccgacaagg cgcacatttc
     1681 ccgcatgact catcctgtat gaattgcata aagaacttag cgaggtcaac catgcaggtg
     1741 tcctcatcca tgacaatgag ccctcctgag cccatcatcg tgcccagttc tttcagggat
     1801 tcgtaatcga taggtgtgtt tagatactga gcagggatgc atcctccgga gggacctccg
     1861 gtctgtgcgg ccttgaactt cttgccattg gggatgcctc cgcctatgtc gaagataatg
     1921 tcgcccagtg gcgtccccat cgggacttcc acaagtcccg tgttgtttat cttgcctgca
     1981 agagcaaata cctttgtgcc cttgctcttt tcggtcccga tagatgaaaa ccattctggg
     2041 cctttgagaa taatcggcgc aatgttggca tatgtttcaa cgttgtttat caacgtcggc
     2101 tgcccccaca gacctgactg agcaggatat ggaggcctcg gcctcggctc gcctcgccga
     2161 ctctctatag acgccagaag agcggtttcc tcaccgcaca caaaagcacc tgccccaacc
     2221 cggaggtcga tatcgaaatc gaatccggat ccgagaatat ccttgcccag caagccgtat
     2281 tcgcgcgctt gtcctatcgc atgttgcagg tgctcaacgg ccaaaggata ctctgccctt
     2341 acgtatatat agccctggtt tgcaccaacc gcatagcctg caatcgtcat ggcctccagc
     2401 actgcatggg gatcgccttc cagcactgac ctgtccatga aagcacctgg gtctccctcg
     2461 tccgcattac acaccacgta ttttggagaa cccggcgcct tggctgtcag cagccactta
     2521 gtccctgtgg ggaacccagc gccaccccgg cccctgaggc ccgaacgctt tatcacatcg
     2581 actacatcgt caggcgtcat ctctgtaaga gccttgccca aggcggcata accgtctctg
     2641 gcaatatact catctatctc cagcggatcg attactccgg tgtttcggag agctattcgt
     2701 tcctgccggc tgaagaaagt catgccttcc tgagtttcca ccttttccga tgtcatcggt
     2761 tcctcataaa ggagtcgact tacaatcctg cctttcacaa gatgctcatc aactatctcc
     2821 ctggcatcat caggcgtaca attgcagtaa agaacgcctt caggataaac aacgattatc
     2881 ggaccaagat cgcatggtcc catgcatcct gtgacgacaa ctttaatctc gcgcccaatg
     2941 cctttcgcgg ctatttcagc ctccagagcc tcgcgaacct ctgcacaccc ggaggacaca
     3001 caggctgcgc cgccgcaaac aaggacgtgc gctctaacca aatccattcg tattcctccc
     3061 tcgttccctt gctctgccat ccttatcagg aatacttcgc caaaacctca gccacgcggt
     3121 cgggcttgat gcgcgcatag atgtcatcat caaccgtcat gactgggcca agaccgcagg
     3181 cgccaataca cctcatcacc tgaatcgaga acatcctgtc atcagttgtg tctccagcct
     3241 tgatccccag cgcctgttct accttttcga gaatcttgtc gcctccacgc acgtagcacg
     3301 ctgttcccag gcagactcct acctgatgtt tgcccttggg cttggtggaa aacagcgcgt
     3361 agaaggtaac gacgctatag acgtcagcca acggaacatt gagtcccgca gccaccctct
     3421 cctgaacctc caggggaagg tagcccacca cttcttgaat ctcgtgcaga cacgggatca
     3481 agattccgcg ctggcccttg tacttggcga ttatcccatc tacctgagcc cactgctcag
     3541 gcgtgatatc gccgacgcac ttggattcac agcaacccat cgacatttca acctcctctg
     3601 ccggccctaa tcctcgacat aacatgaatg catattagat gccacatggg ccgataatct
     3661 gctccattct tgctttgtta agtccactgt ggtaatgtgt t
//
LOCUS       DAOSAI010000258         3691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15897_length_3691_cov_3.902090, whole genome shotgun sequence.
ACCESSION   DAOSAI010000258 DAOSAI010000000
VERSION     DAOSAI010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15897_length_3691_cov_3.902090"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..721)
                     /locus_tag="PLM83_08455"
     CDS             complement(32..721)
                     /locus_tag="PLM83_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK71470.1"
                     /translation="MISSDKCECNSNSESFEGAEAVAHRSGKSDTAQVPDGSRVAGQA
                     CDKRARRVTIREIAEAAGVSVSTVSHVLNEYGDISLDTERRVRQVMKEMNYYPSALAR
                     RLVKKRSYLFQLLIFSAEGLHHPFFYEVICGVTDEAEKAGYELLLSVKDSGDRRWRDS
                     LKRCYESKVEGLIVMGSLPGPELFEEIGVGGIPTVFIDIPYEGPRATYVSSDNVCGAR
                     MATEDQVRNVL"
     gene            complement(1334..2521)
                     /locus_tag="PLM83_08460"
     CDS             complement(1334..2521)
                     /locus_tag="PLM83_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71471.1"
                     /translation="MWTDFVNIWRKEMVTTVRDRKGMTQAILVPLIIAVFYASFTPLL
                     NKAMFERSQKPLIIPAKGVAYVDSGFLDTMREFGITLEEYEGDLEAVIRRGDKESGLI
                     FEPGFDENVAEERPAQIRLVTNGTAGGIFGGGFSDSRLQLAVSVFNQKAAAQRVAVRG
                     IDPSALVPVVLETEDVATPEQEGALWASFYLPILVAVVLAQGGLFVAIDVTAGEKERG
                     TLDALLVTPASDWAILLGKMAAVFVIGLIPLLLSVTAFWVVSNMMPSVAAVSQQGLPV
                     FVLWGSLLIGIPMALVMSVAQMIVSVRARTFRDAQSAASPLVFAMMIPGFVAAFAPPK
                     ALWPCLIPLYGPFAALSRMAQAGKLQWDSLALATIGSLVLAVILMTVAVKLFDRERLL
                     YGA"
     gene            complement(2529..3371)
                     /locus_tag="PLM83_08465"
     CDS             complement(2529..3371)
                     /locus_tag="PLM83_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK71472.1"
                     /translation="MIEVRDLVKHYGDVRAVDGVNFVCSDGQITGLLGPNGAGKTTTL
                     RMICGLIRPTRGTVVVDGHDVIQDPASVRRVLGVQTDSSGVYPRLTPREHLRYYGAFY
                     GIAGDRLEQRIQEVIDALDMGEFADRRAEGFSRGQRQKVVLGRALVHDPKNIIMDEPT
                     TGLDVMAVRETRSMIRALRDKGHCVLFTTHYMDEAARLCDRIAVIIRGRIAAFGSPRQ
                     LMEQTGRDSLEDAFVELAGGGEGLLVSGDAGNGDNDDQTIVANGLDGCTEDSARVGAA
                     VDRR"
BASE COUNT          914 a         1092 c          859 g          826 t
ORIGIN      
        1 acaaacatgc tccagtagag gagaactgtt tttacagcac atttcggact tgatcttcgg
       61 tcgccatccg cgcaccacat acattgtcag aggatacata agtagcacgg ggcccttcat
      121 acggaatatc aatgaatacg gtggggattc cgccgacccc gatctcctcg aacagctcag
      181 ggccgggcag cgatcccatg acgataagac cttcgacctt cgattcatag catcgcttga
      241 gggaatcgcg ccatcgcctg tcgcccgaat ccttgacgct gagcaacagc tcgtatcctg
      301 ccttctcggc ttcgtccgtt actccgcata taacttcata gaaaaaggga tggtggaggc
      361 cttctgctga gaaaatgagc aattggaaga ggtagcttcg tttctttacc agtcgcctgg
      421 caagcgcact cggatagtaa ttcatctctt tcatgacttg ccgaacacgg cgttccgtat
      481 cgaggctaat atccccatat tcgttcaaga catgggatac tgtgctcaca gatacgcctg
      541 cagcttcagc aatctccctt atggtgacac gtcttgcgcg tttgtcgcat gcttggccgg
      601 caacacgtga gccgtccggg acctgggccg tatccgactt tccactccga tgcgctacag
      661 cctctgcgcc ttcaaaactc tcgctattgg aattgcactc acatttgtct gagctaatca
      721 tggccgcctc ccatgctccc gaaggtctgt agtaacattc ttcaagcaac gtgcgcaata
      781 cgacggtcaa accgggaacc cagacccgcc gaacatgctt gcgcgcaagt ggcgggtagt
      841 gcatgtgtgt ataaatggct ggcatttgta ttgataaatc gctaaaatag catttactat
      901 aacgttatag tcagtaagac aatagtacaa tatggttccg cttatgtcaa tagaaccctg
      961 cgctaaacca gcatgacata atctgcctag ttttgtgatc gggatgcaag cctttgcata
     1021 acaccgtttc ctatgggtaa tgttcaagca gtgtaagagg atcgtgcagg atgattcgaa
     1081 tgtggaaaca agcgggaaga agttgtttgg agcgatactc aggcgttcgg gatagccggg
     1141 gacgaaatgc ttatctgagt atactttcgc aaacaaacaa ctactcagct gatcatgaag
     1201 gccgtttaca tccccagtat tgagcatctc gtgggaatac tctaacggct gatattagcc
     1261 cggcgcaggc caaccgggga aagccgaggc tacgaagagc tcgagaatga cgcaacacct
     1321 tatcaagact tagctaggcc ccgtaaagca gcctttctct gtcaaaaagc ttgaccgcca
     1381 cggtcatcaa gatcactgcc aagacaaggc tgccaatagt cgcaagcgca agcgaatccc
     1441 actgcaattt gccggcttgc gccatcctgc tcagagcggc aaacgggccg tatagaggga
     1501 tcagacacgg ccatagggct tttggaggag caaaagcagc gacgaatccg ggaatcatca
     1561 tggcaaacac gagcggactt gcagccgatt gcgcatcacg gaaggttctt gctcgaacgc
     1621 ttactatcat ctgtgcaaca ctcattacca atgccatagg gatgcctatg agcaacgaac
     1681 cccagagcac gaaaacgggc aagccttgct gcgatacagc ggccacagac ggcatcatgt
     1741 tgcttacgac ccagaatgcc gtcacgctga gcaaaagagg aatgagccct ataacaaaaa
     1801 ccgccgccat cttgccaagg agaatcgccc aatcagatgc aggcgtgaca agcaaagcat
     1861 ctagagttcc cctctccttc tcgcccgcag tcacatctat tgctacaaac aagccgccct
     1921 gggccaacac caccgccaca agtatcggaa ggtagaaaga ggcccaaagc gccccctcct
     1981 gctccggagt cgcaacatcc tctgtctcca aaaccaccgg cacaagagct gatggatcta
     2041 tcccccgaac tgccacacgc tgtgcagccg ccttctggtt gaaaacgctc actgccagct
     2101 gaagacggct atcggaaaac ccacctccaa agatgccgcc tgccgttccg tttgtcacaa
     2161 gtctgatctg agcaggcctc tcttcagcta cattttcgtc aaatcctggc tcgaatatca
     2221 atcccgattc cttgtcgccg cggcgtatga ccgcctcgag atctccctca tactcctcga
     2281 gtgtaatgcc gaattcccgc atcgtgtcca gaaacccact atccacatac gcaacccctt
     2341 ttgccggaat aatcaggggc ttctggctcc tctcgaacat cgccttattc aacagtggcg
     2401 taaaactggc atagaagacc gcaatgatca gcggcacgag tatggcctgg gtcattccct
     2461 ttcggtcacg tactgtagta accatctcct tgcgccaaat gttcacaaaa tccgtccaca
     2521 tgctctcctc acctccggtc caccgcagca ccgacccgag cagaatcctc tgtgcatccg
     2581 tcaaggccat tagccacaat cgtttgatca tcgttgtcgc cgtttcccgc atctccgctc
     2641 accagcagcc cttcgccgcc cccggcaagt tcgacgaagg cgtcttcgag cgagtcccgg
     2701 cctgtctgct ccatcagttg gcgcgggctg ccgaacgcgg ctatcctgcc gcggatgatt
     2761 acagcgatcc tgtcacacaa tcttgcggct tcatccatgt aatgagtggt aaagagcacg
     2821 caatggccct tgtctctaag agcccgaatc atgctacggg tctcccgcac agccatcacg
     2881 tcaaggccag tggtgggctc atccatgatg atgtttttgg ggtcatgcac gagggcacgg
     2941 ccgagaacta ccttttgcct ctgcccgcgg ctaaaaccct cggccctcct gtcagcgaat
     3001 tctcccatgt ccaaggcgtc aatgacttct tgaatcctct gctccagcct atctccggca
     3061 attccgtaga acgcaccata gtacctgaga tgctctctcg gagtgagtcg cgggtaaacg
     3121 ccgctcgaat ccgtttgcac tcctaagacc ctgcgaaccg acgcagggtc ctgtatcacg
     3181 tcatgtccgt caacgacaac agtgccacgc gtcggacgaa tcagcccaca tatcatccta
     3241 agcgtggtcg tcttccctgc cccgttcggg cccagcaatc ctgttatctg gccatcagaa
     3301 cagacgaaat tcacgccgtc aacagccctt acatctccgt agtgcttgac gaggtccctc
     3361 acttcgatca aacgattcca ccctttcgac gtccgccttc ctatttattc catttaaagg
     3421 ttttccccta cacgattatg gcgtgccacc gtctactgca taacaaagta tgaacaaggc
     3481 actaacacaa ttgccaaaat cataccacat atgtcggggt aaatctattg gagagaactc
     3541 cattgcagca gcaagttgcc aaaatcagta gctgtataaa tagttgagta tgatgtcgac
     3601 gatctcagca actcgtctag catcaacacg gtccggcgta tcagcaggcc cgtggaagcc
     3661 gtaactgtag tcagggtgaa tgaggcttac a
//
LOCUS       DAOSAI010000259         3688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15913_length_3688_cov_2.857143, whole genome shotgun sequence.
ACCESSION   DAOSAI010000259 DAOSAI010000000
VERSION     DAOSAI010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15913_length_3688_cov_2.857143"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..983)
                     /locus_tag="PLM83_08470"
     CDS             complement(60..983)
                     /locus_tag="PLM83_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK71473.1"
                     /translation="MKELLVISGKGGTGKTSVVASFAALAENKVLVDCDVDAADLHLL
                     LSPEVVESHEFMASRQATVDVSKCTGCGHCSEVCRFGAIAMAYGHPTIDPISCEGCTV
                     CSHICPEGAITMVDVMSGHWYVSHTRFGPMVHAKLKAAEENSGKLVSQVRRKAREIAE
                     SQGHELIITDGPPGVGCPVISSISGVDLALIVTEPTVAGVHDMERVLDLTCHFGVESA
                     VCINKCDLDEANAERIEDYCCARGIEIAGKIPFDEEMVRAVVRGEPPVLYGDGAPDCA
                     GPDSVAGEAAQAIRQLWDRVAVLLARDAGSF"
     gene            complement(980..1879)
                     /locus_tag="PLM83_08475"
     CDS             complement(980..1879)
                     /locus_tag="PLM83_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK71474.1"
                     /translation="MIVSIASGKGGTGKTTVAVNLALALEGAMPIRFLDCDVEEPNAH
                     LFLHPEIERSEKVSLPVPVVDESKCDGCGKCARVCAFHAILAFSSRTIVLPELCHGCG
                     GCALLCPQGAITESPKVIGEVETGRAGDIRFAHGRTGIGSPLAPPVVKAVREALDEEG
                     LGAGLTIIDAPPGTSCPVVASIKGSDYCVLVTEPTPFGLNDLGLAVGLVRELGIPCGV
                     VINRAGLGDEAVSAYCSREGIPVLLEIPYDRKYAECYAKGGRLVDDFPELRGALKGLW
                     RKISASGESGAEGEDGRREMGEI"
     gene            complement(1977..2384)
                     /locus_tag="PLM83_08480"
     CDS             complement(1977..2384)
                     /locus_tag="PLM83_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOK71475.1"
                     /translation="MKIAVATDGSTVSPHFGHCSEFTIVTVENGKAVDSAILPNPEHQ
                     PDFLPQYLSEHGVSCIIAGGMGPRAQQHFAARGIKTVIGASGSVDDVVHAYLAGSLEV
                     GESACDHGHGHEQHGECGHGENGGTHRRGCGHN"
     gene            complement(2498..2884)
                     /locus_tag="PLM83_08485"
     CDS             complement(2498..2884)
                     /locus_tag="PLM83_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOK71476.1"
                     /translation="MKLAVTAQGTSLSAGLDPRFGRCEHFVIIDVDTEEFEAFSNESV
                     MASGGAGTESAQFLADKGAEAVVTGNVGPNAAMALEAAGIKVYRMSSGTVKDALEAFK
                     SGTLEAISGPTVNSHHGMGGHGRGGR"
     gene            complement(3009..3419)
                     /locus_tag="PLM83_08490"
     CDS             complement(3009..3419)
                     /locus_tag="PLM83_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOK71477.1"
                     /translation="MPRGDGRGPTGFGPMTGRGAGFCTGYDVPGYANPAMGRGQGFGR
                     APRCYGGGGFGRGYRRMYYATGLPRWMRSGAGAWGQAAYGPAYSPYAQEAMRPSKEQE
                     IGYLKRQSEFLVEELEAVRGRLSELSGSQEYDEG"
BASE COUNT          766 a         1239 c          881 g          802 t
ORIGIN      
        1 cgccgttgtt ggagccatcc tcagctacct tttttcactg ttcgggtaag cctccactcc
       61 tagaaagagc ccgcatcacg cgcgagaaga acagcgaccc tatcccaaag ctggcgtata
      121 gcctgcgcgg cttcgcccgc aacgctatct gggcccgcgc aatcaggagc accatctccg
      181 tacagaacag gcggctcacc ccttacaacc gcccttacca tctcctcatc gaacgggatc
      241 ttccccgcaa tctcgattcc ccttgcacaa cagtaatcct ctatgcgctc agcattagcc
      301 tcatcaagat cgcacttgtt gatacatacc gccgactcta caccgaaatg gcacgtaagg
      361 tcgaggaccc gctccatgtc gtggaccccc gccaccgtgg gttccgttac gatcaaggca
      421 agatccactc cggagatgga ggatatgacg gggcatccca ccccaggcgg cccgtcggta
      481 atgatcaact cgtgcccctg gctttcggct atctcccgcg ccttgcgcct cacttgggat
      541 acgagcttcc ctgagttctc ttcagccgcc ttgagcttgg catggaccat cggcccaaac
      601 cgggtatggg atacgtacca gtgccctgac atgacgtcga ccatggttat agcaccctcg
      661 ggacaaatat gggagcacac agtacaccct tcacaggaaa tcggatcgat ggttggatgc
      721 ccataggcca tggctatagc gccgaagcgg cacacttcgc tgcaatgacc acaccccgtg
      781 catttgctca catcgacagt tgcctgtcgc gaagccataa attcatggga ttcaactacc
      841 tctggcgaaa gcaagaggtg aaggtcggca gcatccacat cgcagtcgac taagactttg
      901 ttctccgcca aagccgcaaa tgacgcaact acactcgtct tgccggtgcc tcccttgccg
      961 cttatcacga gcaactcctt catatctcac ccatctccct tcgcccatcc tctccctctg
     1021 cgccagattc gccgctcgca ctgatcttgc gccacaaccc cttcaaagcg cctcggagct
     1081 ctggaaagtc atccacaagc cttccgccct tagcgtagca ttcggcgtac ttcctgtcgt
     1141 atggtatttc aagcaacaca gggatgccct cgcggctgca ataggctgaa acggcctcat
     1201 cccccaaccc ggccctgtta atcactacgc cgcagggtat gcctagctcc cgaaccagtc
     1261 caacggccag cccaagatcg ttgaggccga agggcgtagg ctccgtcacc agcacgcagt
     1321 aatcgctgcc cttgatcgaa gccacaaccg gacaggacgt cccggggggc gcgtctatga
     1381 tcgtcaaccc ggcccctagg ccttcctcgt ccaacgcctc cctcaccgct ttcaccacag
     1441 gcggtgccag agggcttccg atccctgtcc gtccatgggc aaacctgata tctcctgcgc
     1501 gccccgtctc cacttccccg atcaccttcg ggctctccgt tatcgccccc tgcgggcaaa
     1561 gaagcgcaca ccccccgcat ccatgacaga gctcgggcag gacgatcgtc cgggagctga
     1621 aggcaaggat cgcatggaaa gcgcacaccc gggcgcattt tccgcatccg tcacacttgc
     1681 tctcgtccac aaccggaact ggcaaactta ctttttcgga gcgctcgatt tcgggatgga
     1741 ggaaaagatg ggcattgggc tcctcaacat cgcagtccaa aaagcgtatc ggcatagccc
     1801 cttccaaggc caacgccaag ttgaccgcga cagtagtctt gcccgttccc cctttgccgc
     1861 tcgctattga aacaatcaca ttccatcgcc accattcatc gtgcatgccc caacttcgcg
     1921 tattttgtcc ttccaaaaac aggcaggctc taacgggcct gcccgccaat cccccgctag
     1981 ttgtggccgc agccgcgcct atgggttccc ccgttttccc cgtgtccgca ttcaccgtgc
     2041 tgctcatgtc cgtggccgtg atcgcaagcg ctctcgccca cctcaagaga tcccgcaaga
     2101 tacgcgtgga cgacatcgtc cacactcccg gacgctccta tcacggtctt aatgccccta
     2161 gcagcaaagt gttgctgggc ccttggaccc attccgcctg cgatgatgca tgaaactccg
     2221 tgttcagaaa ggtactgagg gagaaaatcg ggttgatgct cggggtttgg aagaatggcc
     2281 gaatcgacgg ccttgccgtt ttccactgtg actatggtga attcagagca atgcccgaaa
     2341 tgaggcgaga cggtggaacc gtcagttgcc acagcaatct tcatctatgg ttaccccctt
     2401 aaatgtgaaa agaccaattc tgctcgatcc tatcgtggga agctccgttt gcgccgacgg
     2461 ggtctgcccc acttgccagc tggcccagcc cttgagccta gcgacccccg cggccatgcc
     2521 cgcccattcc atgatgggaa ttgactgtag gtccggaaat tgcctcgagc gtccctgact
     2581 taaaagcctc caaagcgtcc ttcactgtcc cggaagacat tctgtagacc tttatgcccg
     2641 cggcttccag agccatcgct gcattgggac caacatttcc ggtaacgacc gcctctgcgc
     2701 ccttgtcagc aagaaactgg gcgctttcag ttcccgctcc gcctgacgcc ataacgctct
     2761 cgttggagaa tgcttcgaat tcctcggtat cgacgtctat gatcacaaaa tgttcgcacc
     2821 tgccgaaacg cgggtccaac ccggctgaga ggctggttcc ctgcgccgtt actgctagct
     2881 tcaaactacc atctcccctt aacctccggc aactttgtcc actatcctcg gtacaggtcg
     2941 attgctggcc ggcgaaggag ccgaaggacg ctcaaatccg acctctctca ccccttcgcc
     3001 tcgaaggact agccttcatc gtactcttgg gaaccagaaa gctcgctcag acgcccccgg
     3061 acggcctcga gctcctccac gagaaactct gactgtcgtt ttagatagcc tatctcctgt
     3121 tctttcgacg gcctcatggc ctcttgggcg taagggctgt aagcaggccc gtacgcggcc
     3181 tgaccccatg cccctgctcc agaacgcatc catctcggca gtcccgtcgc gtagtacatc
     3241 cttctgtacc ctcgtccgaa tccgcctccg ccataacagc gcggagcgcg tccaaatccc
     3301 tgtcccctgc ccattgcggg attagcatat ccaggaacat catatccggt gcagaagcct
     3361 gcgcccctgc ctgtcatagg accgaaacca gttggtcctc ttccatctcc tctcggcatg
     3421 taaagcacct cctctccaaa gccttggggt ttgatcgaac gactacctcc acggcttttt
     3481 catatgccca tcctccggtt ggattgaata cgtgcttggc ttgacgccat caattgctgt
     3541 tgtagggtta tgggcatatg ttcattacca gtatacccat ggttgtgagc atatgtcaat
     3601 aatctgagcg cagaaatgtg ccggcaatgg ttcagttccc atcgttggcg aaaaaatagg
     3661 cccgtccttc tcggcggatg gcgcagat
//
LOCUS       DAOSAI010000260         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_15954_length_3680_cov_3.310069, whole genome shotgun sequence.
ACCESSION   DAOSAI010000260 DAOSAI010000000
VERSION     DAOSAI010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15954_length_3680_cov_3.310069"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..3007
                     /gene="alaS"
                     /locus_tag="PLM83_08495"
     CDS             368..3007
                     /gene="alaS"
                     /locus_tag="PLM83_08495"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOK71478.1"
                     /translation="MKTDELRQAYLRFFESKGHKILPSAPLVPAGDPTLLLTAAGMVP
                     FKPYFLGTAVPEHTRVTTCQKCVRTGDIDNVGKTTRHGTFFEMLGNFSFGDYFKAEAI
                     PWAWEFVTDVLGLAPENLWVTIYLDDDEAFNIWRDVVGVSADRIIRKGKKDNFWEIGV
                     GPCGPCSEIYIDRGADVGCGSPECGLDCDCGRFIELWNLVFIQYHKDEDGNYTPLERP
                     GIDTGMGLERVAAVLQGAETIFDTDETRVVRDRAAQLACVEYGADHQSDVAIRVITDH
                     VRAAAFMVADGIFPTNEGRGYVLRRLLRRAARYGRLVGIEGSFLPQVADTVIDVMAAP
                     YPELARDRDRILKAIALEEERFAAALEQGSVVLESIIAEVERDGGRMLPGEDAFKLYD
                     TYGFPFELTEEIASERGLQVDKAHFDEAMEAQRERARAARGENAYLDEKSAKYHDAIG
                     DLTTEFVGYHEFADTANIIAIIGKDGNSLAELSEDQDGETVMNKTPFYPTGGGQQADT
                     GLIVSSRGAVAEVCSVSRPFESVITHQVHMIRGTLKVGDRVIAYVDEDRRAATMRNHT
                     ATHLIQAALRQVLGEHVQQAGSYVDGERLRFDFSHFEALTVDELAQVELLANRWVMAD
                     IPVDVRMTTIDEAHRLGAIALFDEKYGDEVRMVGIGDISLELCGGTHVSRTGEIGLVK
                     IVSESGIAAGVRRIEAITGYAFLQRFNDMDREFQSLAEILKVPEDMMVERVEKLLGEL
                     KEKEREIARLRERLAVGGVDSLVAGRIMVDDIPVVAAPVELADPEALRNLADAVRDKL
                     GSGIVLLGSKAEEKVLFVATVSRDLVKQGFNAGSIVREAAKVAGGGGGGRPDMAQAGG
                     RDVSKLEAAIEAGVDVVRAHSAS"
     gene            3045..3308
                     /locus_tag="PLM83_08500"
     CDS             3045..3308
                     /locus_tag="PLM83_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HOK71479.1"
                     /translation="MSGKWDETVKYPAVEAEQEVTPVKKALTEVTIALEEKGYNPVNQ
                     LVGYLLSGDPTYITSHRNARNTIRKVERDEIIEELVRSYLRKE"
     gene            3394..>3680
                     /locus_tag="PLM83_08505"
     CDS             3394..>3680
                     /locus_tag="PLM83_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71480.1"
                     /translation="MSNHALASAIAAPNGERFEDFPGRRGVIVIIADGFSLRDALEHG
                     GPWLLSFLDSASIGMSNCRTADGGDRLLSAAATFGAGRRMSARESAGYGLG"
BASE COUNT          825 a          849 c         1168 g          838 t
ORIGIN      
        1 gcttgaattg ttagcctttt gggcagccgt tgacgtggtg ggcctggcgt tttgcgggta
       61 aagtggtctt cttcgtaccg gagtggttta tgaatcgttt ttggcgattt gaagtggaaa
      121 tccagcttcg tcctgtggag attggattcc gtatgcctaa cgcatgagga agcctgcaat
      181 ctgctatggc acagtggcca tttcagttat tcatagcgcg aagcctgtgc acgcggagga
      241 tgggagcgga gccgtatgcc gtattgtgcg agcagcggcg ttatgataat gtacttgagt
      301 agacttacga gagtttgcag aagcatcccg gctatgcggc aggggctttc tagatggggt
      361 gtaatatgtg aaaaccgatg aactaaggca agcgtatctc aggttttttg aatcaaaagg
      421 gcacaaaatt ctgccgtcag cgccgctcgt tccggcgggc gatccgaccc tgctcctaac
      481 agccgcgggg atggtgccgt ttaagccgta ttttctgggc actgcagtgc ccgagcacac
      541 acgggtcaca acgtgccaga agtgtgtgcg aaccggcgac atagacaatg tggggaagac
      601 tacccgtcac ggcactttct ttgagatgct gggcaacttc tcatttggtg actacttcaa
      661 agccgaggca ataccgtggg cgtgggagtt cgtcacggat gtcttgggtt tagcccctga
      721 aaacctttgg gtaaccatat acctcgatga tgacgaagcc tttaatatct ggcgcgatgt
      781 ggtaggtgtt tctgcagatc gcattatcag gaaaggcaag aaggataact tctgggagat
      841 aggggttggg ccatgtggcc cctgttccga aatatacatt gaccgtggcg ccgatgtggg
      901 gtgcggatct cctgaatgcg gtcttgactg cgactgtggc agattcattg aactgtggaa
      961 cctcgtcttc atccaatacc ataaagacga agatggaaac tatactccac ttgagcggcc
     1021 gggaatcgac actggaatgg gacttgaaag ggtcgcggcc gtgcttcagg gggctgagac
     1081 gattttcgac accgatgaga ctcgcgtggt gcgagatcga gcggctcagc tggcctgtgt
     1141 agaatatggt gccgaccacc agagtgacgt ggcgatcagg gtgattactg accacgttcg
     1201 tgccgccgct ttcatggtag cggatgggat tttcccgaca aacgagggac gtggatatgt
     1261 tctgcgccgc cttctcagac gggctgccag atatggaaga cttgttggaa tcgagggatc
     1321 cttccttcca caagttgctg atacggtcat agatgtaatg gccgccccat atccggagct
     1381 tgcacgcgat cgcgatcgca tactgaaggc catagctctc gaggaagaaa ggtttgctgc
     1441 agcgcttgaa caagggtctg tagttttgga atcgattatt gctgaggtcg aacgggatgg
     1501 gggtagaatg ctccccggtg aggatgcttt caaactttat gacacgtacg gttttccgtt
     1561 cgaactgacc gaggagatag catcagagcg tggcctccaa gttgacaagg cccacttcga
     1621 tgaggcaatg gaggctcaga gagagcgggc gcgtgccgct cggggcgaga atgcctacct
     1681 tgatgagaag agcgcgaagt atcatgacgc aatcggagat ttgacaacgg aatttgtggg
     1741 gtaccatgag tttgcggaca cggccaatat cattgccata atcggaaaag atgggaacag
     1801 ccttgcggag ctctccgagg accaagatgg cgagacagtg atgaacaaga ccccgttcta
     1861 cccgaccggt ggtggacagc aggctgatac gggattgata gtctcgtcaa ggggtgcagt
     1921 ggcggaggtg tgcagcgtca gccgaccgtt cgaaagcgtc attacccacc aggttcatat
     1981 gataaggggc acgctgaagg taggcgatag agtcattgcg tatgttgatg aagatcgcag
     2041 agccgccacc atgcgaaacc acacggccac acatcttatc caggctgcgc tcagacaagt
     2101 cctgggagaa cacgtgcagc aggccggttc atacgtggac ggcgagcgac tcaggtttga
     2161 cttttcgcat tttgaggcac tcaccgtaga cgagcttgcc caagtcgagc ttttggcaaa
     2221 tcgatgggtc atggcggaca ttcctgttga cgtgaggatg actactatcg acgaggccca
     2281 taggcttggt gcaatagcac tctttgatga gaaatatggc gatgaagtcc ggatggtggg
     2341 tatcggcgat atcagtctgg aattgtgcgg cgggacgcat gtgtcccgaa caggagagat
     2401 cggcctcgtc aaaatcgtgt cagaaagcgg tattgcggca ggagttcgca gaatagaagc
     2461 gataaccggt tacgcgttcc tccagcgttt caacgatatg gatagagagt ttcagagctt
     2521 ggctgagatt cttaaggttc ctgaggatat gatggtcgag cgcgtggaga agctcttggg
     2581 cgagttgaag gagaaggagc gggagatcgc gcgcctccgt gagcgcctgg ccgttggcgg
     2641 cgtggattcg ttggttgccg gcagaatcat ggtcgatgac attcctgttg tagctgcccc
     2701 tgtcgaactg gccgatccgg aagctttgcg caatctggca gacgctgtgc gagacaagct
     2761 aggttccggc attgtactgt tgggatctaa ggcggaagag aaggttcttt tcgttgcgac
     2821 ggtcagccgc gatcttgtga aacaaggctt taacgcgggc tcgatagttc gcgaagcggc
     2881 caaagtagct ggtggaggcg gaggtggcag gcccgatatg gcccaagctg gaggaagaga
     2941 tgtctctaag cttgaggcgg cgatcgaagc cggcgtcgat gtggttcggg cgcattctgc
     3001 gtcctgatga ggaaagacta aatctgcccg gggtggtgtg gtttatgagt ggtaaatggg
     3061 atgaaacagt gaagtaccct gctgtcgagg cggaacagga agtcactccg gtaaagaaag
     3121 cccttaccga agtgacgatt gcccttgagg aaaaggggta caaccccgtg aaccagctcg
     3181 tgggttacct cctttcgggc gatccgacat atattacgag tcacagaaac gcgcgcaaca
     3241 ccattcgcaa ggtggagcgt gatgagatta tagaggagct ggttcggagt tatctcagga
     3301 aagaatgatc gagtggcgcc cccacgctgg gcgccttttt ccgtctccaa aactggaacc
     3361 tgcgttgtta tccttgtcgc gttgttgtgc gcagtgtcaa accatgcctt ggcgtccgcc
     3421 atagcggcgc cgaacgggga gaggttcgaa gatttcccgg gtcgacgcgg tgtcatagtt
     3481 ataatagccg atggcttctc attgagggat gccctggagc atggtggccc ttggcttctg
     3541 tccttccttg actcggcatc catagggatg tccaactgcc gaacggcaga cgggggcgac
     3601 aggctccttt cggcagcagc taccttcggc gccgggcgcc gaatgagcgc ccgcgagtcg
     3661 gctggctatg ggctgggcag
//
LOCUS       DAOSAI010000261         3666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16043_length_3666_cov_3.391581, whole genome shotgun sequence.
ACCESSION   DAOSAI010000261 DAOSAI010000000
VERSION     DAOSAI010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16043_length_3666_cov_3.391581"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1229)
                     /locus_tag="PLM83_08510"
     CDS             complement(15..1229)
                     /locus_tag="PLM83_08510"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOK71481.1"
                     /translation="MSYEVTTIESICSRVLSGGTPRRSVPEYYRGGCIPWLNTKEIDY
                     NRIYKTENHITPLGLERSSAKWIAPNSVIVAMYGATAGKVAINKIPVTTNQACCNLEI
                     DSRKADYNYVYYYLKWKYPELSNLASGAAQQNLNVGIIKAFPISLPPLEVQKVIGETL
                     SCLDAKIDLNDRMNENLEKQAQAIFKSWFVDFEPFHYGEFVDSVIGPIPKGWKACAVT
                     DVIDVNPKRKLDKGTSAKYLSMANVSTSGPRVLGWEQRNFTSGTCFMNGDVLLARITP
                     SLEHGKTAFVDFLADQEVGWGSTEFIVMRSREPLPLEYTYCLAKHEPFREHMIKSMTG
                     TSGRQRAQVDALKHYFLAIAPKDVYAEFQEIVIPFFLAIRRNATENQVLAALRDTLLP
                     KLMSGEIEVPVV"
     gene            complement(1219..2721)
                     /locus_tag="PLM83_08515"
     CDS             complement(1219..2721)
                     /locus_tag="PLM83_08515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOK71482.1"
                     /translation="MSRINGADVGFEQEIWKAADVLRGNMDASEYKHVVLGLIFLKYI
                     SDAFEQRYQELVAEGKGYEEDRDEYTYLNIFFVPEEARWSRIAENAHTPEIGVIIDKA
                     MQAIERENARLRGILPKNYARPELDKRRLGDIVDIFTNVSMKEHGDTKDILGRTYEYM
                     LSKFAEQEGRNAGEFYTPVCLVKTLVEILEPYKGRVYDPCCGSGGMFVQSSRFVQSHQ
                     GNLQDISIYGQEANPTTWKMCQMNLAIRGIEANLGPGPDDTFFNDLHPTLKADFILAN
                     PPYNMSGWGADRLQDDKRWIYGVPPAGNANFAWLQHMIYHLSARGKMGVVLANGSLSS
                     QTAGEGEIRRRIIEADLVECIVAMPPQLFYSTPIPVSLWFINKHKKQPGKTLFIDARK
                     MGKMVTRSLRELTDDEVRELANTHKAFEAGTFEEVKGFCAVATIEEIAEHDYILTPGR
                     YVGIEEPEDDGEPFEEKMERLTAELAELFKHSSELEQEIRNRLGAIGHVV"
     gene            3149..3622
                     /locus_tag="PLM83_08520"
     CDS             3149..3622
                     /locus_tag="PLM83_08520"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOK71483.1"
                     /translation="MGQGRIVYTAVTDSPNLFWLVQQFRNATPYDNVPKYIIHDNDPV
                     FKSRNFKKLLDDSCVTAITTGKEAPWQNPYAERVIGSIRRELLDYVIPMNERHLEKML
                     QEYVSKYYNPHHTHQGLGGATPFPHPAYPPTKMADNTLVATPVLNGLYHTLEKAS"
BASE COUNT          888 a          984 c          823 g          971 t
ORIGIN      
        1 ccattctact caagctatac gaccggaacc tcaatctctc cactcattag tttggggagg
       61 agggtgtcgc gcagggcggc caagacctgg ttttcagtgg cattcctcct gatggcgagg
      121 aagaaaggta tcacgatctc ttggaactca gcataaacgt ctttcggggc gatcgccagg
      181 aagtagtgct ttagcgcatc aacctgcgcg cgttggcgtc ctgacgtgcc cgtcatgctc
      241 tttatcatgt gctctctaaa gggttcatgt tttgcgaggc aatacgtgta ttccagaggc
      301 aagggttctc gactacgcat gactataaac tccgtggaac cccaccctac ttcttgatct
      361 gccaagaagt ccacaaatgc tgtcttgcca tgctccaagc ttggcgttat cctagctaaa
      421 agcacgtccc cgttcataaa acaggtgcct gaggtgaagt ttcgctgttc ccaccccaac
      481 actcgagggc ctgatgttga tacgttagcc atactgaggt acttcgcaga agttcctttg
      541 tcgagtttcc ttttcgggtt cacgtcaatt acatctgtaa cagcacaagc tttccaaccc
      601 ttgggaattg gtccgatgac actgtcaaca aattctccat agtgaaaggg ctcaaaatcc
      661 acaaaccagc tcttaaagat ggcttgagct tgcttttcca ggttttcgtt catcctgtcg
      721 ttcaagtcga tcttcgcatc caagcaggac agagtctcac caatgacttt ttggacttct
      781 agaggtggga gcgaaatagg gaaggcctta attatcccca cattcagatt ctgctgcgct
      841 gctccgctgg cgagattgga cagctctggg tatttccatt tcagataata atagacataa
      901 ttgtagtccg cctttctact gtctatctct aggttgcagc atgcttgatt cgtcgtcaca
      961 ggaatcttgt tgatggcaac ctttccagcc gtcgcaccat acatggccac gatgacggag
     1021 ttgggagcta tccactttgc ggaacttctt tctagaccca atggggtaat gtggttctct
     1081 gtcttgtaaa tgcgattgta gtcgatttct ttggtgttca gccatggaat acatccgcca
     1141 cggtagtact cgggtacgct gcgcctggga gtgccgccgc tcaacacgcg actacaaatg
     1201 gactcaatag tagtgacttc atacgacatg tccgatggcc cccaacctgt ttcgaatttc
     1261 ctgttccagt tccgaactat gcttgaagag ttcggccagt tcagctgtca agcgctccat
     1321 tttctcctca aacggctctc cgtcgtcttc gggttcctct atacctacat aacgtccggg
     1381 ggtgaggatg taatcatgct cggctatttc ctcgatagtc gcgacggcgc agaacccctt
     1441 cacttcctca aaggttcccg cttcaaaggc tttgtgggta ttagccagct ctctaacctc
     1501 gtcatctgtt agttcccgca gagaacgggt aaccatcttg cccatcttgc gagcatctat
     1561 gaacagcgtc ttacccggtt gcttcttgtg cttgttgatg aaccataggg acacagggat
     1621 cggtgtcgag taaaagagct gaggcggcat agccacaatg cactctacca ggtcggcttc
     1681 gataatgcgt ctccgaatct ctccttcacc agcagtctgg cttgagagcg aaccattggc
     1741 taggacaacg cccattttac ccctggctga caagtggtag atcatatgct gcaaccaagc
     1801 aaagttagcg ttacctgccg gtggtacacc gtatatccaa cgcttgtcat cctggagcct
     1861 atctgctccc cacccagaca tgttataggg agggttagcc aggatgaagt ctgctttcag
     1921 agtcgggtgc aagtcattaa aaaatgtatc atcagggcca ggtcctaagt tggcttcaat
     1981 gccgcgaatg gccaggttca tctgacacat tttccaggtg gtgggattgg cttcctggcc
     2041 atatatggag atatcctgca gatttccttg atgactctgt acgaacctgg aagactgcac
     2101 gaacatacct ccactgccgc aacaagggtc gtacacccta cccttatagg gctcgagaat
     2161 ttccaccagt gttttgacta agcaaacagg agtgtagaat tctccggcgt ttctgccttc
     2221 ctgctcggca aatttgctga gcatgtactc ataggtgcgg ccgagaatgt ccttagtatc
     2281 accgtgttct ttcatggaaa cgttggtgaa gatgtctacg atatctccca gcctacgctt
     2341 gtccagctca ggccgtgcgt agttcttggg aagaattccc ctgagtctcg cgttttcccg
     2401 ttcaatggcc tgcatggctt tgtcgattat gacgccgatc tcgggagtat gggcattttc
     2461 tgcaatccta ctccagcgag cctcctcggg cacgaagaag atgttcagat aagtatattc
     2521 atcccgatct tcctcgtaac ccttgccttc ggcaactaac tcctgatacc tctgctcaaa
     2581 ggcatcggag atgtatttaa gaaagatcag gcctaagacc acgtgcttgt attccgacgc
     2641 atccatgttg ccgcgcaaca catcagccgc tttccaaatc tcttgctcaa aaccaacgtc
     2701 tgccccatta atcctcgaca atcttcatcc ctccgtactc aacaccttac ttgtcctacg
     2761 acagcatccc agctgctacg ggagctttcc cgcggaatca ttcagcaata gcatgacaga
     2821 tgaaaccagt acgcagaaac aacactataa cagtatattc gacgaagatc ggccgagatc
     2881 ctacttacat tcgtctacag caagacgtcc gtggcatggt caccggaaag aacacaccag
     2941 ctattgattc tactaaacgt tacagaccca cctgccccca gcaccatcag gaagtatctc
     3001 aagttaaaaa aaccgccttc atcacataga aagcggcgag catggtatac attctaccag
     3061 aatcatgctg aggaaatctg gtgagttgac tttttcacgg tacctacttt atcgtttgga
     3121 atcctgtatg tcttggtaat gattcatcat gggacaagga agaatcgttt acactgcggt
     3181 cactgattcc ccaaatctat tttggctggt gcagcagttc cgcaatgcga ctccatatga
     3241 caacgtgcca aaatacatca tccacgataa tgatcccgtt ttcaagtcta gaaatttcaa
     3301 gaagcttctt gacgattcct gtgttacggc aatcaccacc ggaaaagagg ctccctggca
     3361 aaacccctat gctgaacgag ttatcggctc aattcgccgg gaactgctag attatgtcat
     3421 tcctatgaat gagcgtcatt tggaaaagat gctccaagag tacgtctcca agtactacaa
     3481 tccccaccat acacatcaag gactcggtgg cgccacaccc tttccacacc ctgcgtatcc
     3541 gccaacaaag atggcagata atacgctcgt ggcaacacct gtgcttaatg ggctctatca
     3601 taccttggaa aaagccagtt aattagggta gctaaccaaa tgtgcctggt ctttttgtcg
     3661 cgtccg
//
LOCUS       DAOSAI010000262         3645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16163_length_3645_cov_3.723677, whole genome shotgun sequence.
ACCESSION   DAOSAI010000262 DAOSAI010000000
VERSION     DAOSAI010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16163_length_3645_cov_3.723677"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLM83_08525"
     CDS             complement(<1..274)
                     /locus_tag="PLM83_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HOK71484.1"
                     /translation="MRVAVILNPNARHGRAAEDEAEAVRTLADLGVDFDLAKTSGPGD
                     ATSMARRAVEQGRDVVCAMGGDGTVNEVINGIACTGAALRVIPVGIG"
     gene            complement(382..1614)
                     /locus_tag="PLM83_08530"
     CDS             complement(382..1614)
                     /locus_tag="PLM83_08530"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOK71485.1"
                     /translation="MKQESITIREKETAAKIQNTHINAVRTKDITKKGVRVFADGNIG
                     ISGAIGDVAEEKLVENAVQNLSVGIEYPYPISRDRKDHRSYNDNPMTAGELLSVTESI
                     LETLRKEYPDFEFSETISANEIMTEMRNSEGLDLKYEDAHYSLGLILKEKKSANLFDG
                     ILMCVGCRFDMDKFWAFNRPYLEAYRNRVALPEGEVLPVFTMEPDNLMSFLNRSLNGE
                     RFATGSSVFSGKMGEQLFNEKVTLEQNRNPLHQPGAFFDMEGVVLPEDRHVLIESGKL
                     VSVFTDKKNAHLYNLPHTGSASGAYDGMPSLTTVPLRFRTDSRDLKGALKGQPAILTV
                     ISSGGDFTPEGSFAAPVQVGFLFDGERIIGRLPEFTMRSHINKMLGEDYIGTFDNTVF
                     YIGDLPTQVQGYYMTIMK"
     gene            complement(1628..2962)
                     /locus_tag="PLM83_08535"
     CDS             complement(1628..2962)
                     /locus_tag="PLM83_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOK71486.1"
                     /translation="MYKFPKGLYVDVRIEETFDTKISFKKLTLQEQKIRNNRGAFIRV
                     FDGRRWYYSSTTDINGIQAQIDALAQMATPNREILEHPIVKAFEVNQETIMEYDAKSV
                     TDIPAQEKQELLESYLTLITDPTIVHHSSFYADNKTVKSIYTSKGTAVTFDKQTCGVR
                     INLELSCGEKKNQTSISKAGVFFDDLLNLEDFFQTELEKNIAFTRDAEPVIPGEYHVL
                     LGPEATGVFTHESFGHKSEADFMVGDETMKAEWALGKRIGQSLLTIIDDGTVPGNGYV
                     PYDDEGTKGKKTYIITEGILSGRLHSAATAAQLEESLTGNARAVNFEFEPIVRMTTTY
                     IERGNRPLGDIVAEMENGIYVDTIRHGSGMSTFTLAPDRAYKIENGQIAGPVNVSVVT
                     GNVFSTLAEVDAVSEEFELMSFVGGGCGKMEQWPLPVGFGGPYVRVRKLKVQ"
BASE COUNT          797 a          999 c          880 g          969 t
ORIGIN      
        1 tcccgatgcc cactggtatc accctaaggg ccgctcccgt gcacgctatt ccgttgatca
       61 cttcgttgac tgtgccatcg ccgcccatgg cgcatacaac atcacgacct tgctcaacag
      121 ccctccgcgc catggaggtt gcatcccccg gcccagaggt cttggccaaa tcgaaatcga
      181 ctcccaggtc cgccagagtc cgcaccgcct cagcttcatc ctccgctgct cgtccatgcc
      241 tcgcattagg gtttagtatg accgcgacac gcaacttcct gcctcctgtc agtacatagt
      301 gatgaaatcc agatacttct gtgattcaac aatcgcagca tcattcctgc gcaggcatga
      361 gagagggggc cataacggcc cctacttcat tatagtcatg tagtatccct gaacctgggt
      421 tgggaggtcg ccgatgtaaa agacagtatt gtcaaaagtg ccgatatagt cctctcccag
      481 cattttgttt atgtgggagc gcatggtgaa ttccggaagc ctgccgatta tcctctcacc
      541 gtcaaagagg aagcccacct ggacgggggc ggcaaagctg ccctcagggg tgaagtctcc
      601 gccggaactg atcactgtga ggatggccgg ctgccctttc agggctcctt tcaaatctct
      661 ggagtcggtc ctaaagcgca gtgggactgt ggtcaaagaa ggcatgccgt cataggctcc
      721 tgaggcggaa ccggtgtgcg gcaggttgta aagatgggcg tttttcttgt cagtgaaaac
      781 gctgaccagc tttccggatt cgatcaggac gtgacgatcc tccgggagaa caacgccttc
      841 catgtcgaaa aaggctcccg gttggtgcag tggatttctg ttttgctcca aagtaacctt
      901 ttcattgaat aactgttccc ccatctttcc gctgaaaaca gagctcccgg tggcgaagcg
      961 ttcaccgttg agagacctgt tcagaaagct catcagatta tccggctcca tggtgaatac
     1021 aggcagcact tccccttccg gcaaggccac cctgttgcgg tatgcctcca gataggggcg
     1081 gttgaaggcc cagaacttat ccatgtcaaa cctgcagccc acacacatca aaatgccatc
     1141 gaatagattg gcagacttct tctccttgag gatcaacccc agggaatagt gagcatcttc
     1201 gtacttgaga tcaagtcctt cgctattgcg catctcagtc attatctcat ttgcggaaat
     1261 ggtctcgctg aactcaaagt caggatactc cttgcggagg gtttcgagga tggattctgt
     1321 gacggacaac agttccccgg cggtcatggg gttgtcattg taactgcggt ggtcctttct
     1381 atcccgggat atgggataag gatactcgat gccgacactc aggttctgga cggcattttc
     1441 aaccagcttc tcctcagcca cgtccccaat tgccccggat atgccgatat tcccatctgc
     1501 aaatacccgt acgccctttt tcgtgatgtc cttggtacgt actgcattga tatgggtgtt
     1561 ttgaatcttt gctgcagttt ccttttccct aatggtaatc gattcttgtt tcattgctct
     1621 gcccccctta ctgaaccttg agttttctga cccgaacgta tggaccgcca aatccgactg
     1681 gcaggggcca ctgttccatc ttgccacagc caccaccgac aaagctcata agctcgaact
     1741 cctcgctcac tgcatctact tctgccaaag tgctgaatac attgccggta acaaccgaga
     1801 cattgacagg cccggcaatc tggccgtttt cgatcttgta tgcccggtca ggggcgaggg
     1861 tgaaggtgga cattccggac ccgtgtctga ttgtgtccac atagatgccg ttttccatct
     1921 ccgcgacgat gtccccaagg ggcctattgc ctctttcgat gtaagttgtg gtcatgcgca
     1981 caattggctc aaactcgaaa ttcacggccc tggcattgcc ggtcagggat tcctccaatt
     2041 gggcagccgt ggccgcactg tgcaagcgtc cgctgagaat gccctcggtg atgatatatg
     2101 ttttcttgcc tttggtgcct tcgtcatcat agggaacgta cccattgccc gggacagtcc
     2161 cgtcgtcgat gatggtgagc aagctctggc cgatcctctt gcccagggcc cattccgcct
     2221 tcatggtttc atctcccacc atgaaatcgg cttcgctctt gtggccgaaa ctctcatggg
     2281 tgaatacccc ggtggcttcc gggccgagca agacatgata ttctccggga attacaggct
     2341 cggcgtccct tgtaaatgcg atattcttct ccagctcggt ttggaagaag tcctcaaggt
     2401 tcaaaagatc gtcgaagaat acgccggcct tagagatgct ggtctggttc ttcttctctc
     2461 cgcaggatag ttccaggttg atgcggacgc cgcaggtctg cttgtcaaat gtcacggcag
     2521 tgccctttga tgtgtatatg gacttgacgg ttttgttgtc cgcgtaaaag gacgaatggt
     2581 ggacgatggt cgggtctgtt atgagggtta ggtagctttc gagcaattct tgcttttcct
     2641 gcgcgggtat atcggttacc gatttcgcat cgtattccat tattgtctct tggtttactt
     2701 caaaggcttt aacgatagga tgctcaagga tttctcgatt tggagtggcc atttgggcca
     2761 aggcatcaat ttgagcctgg atgccgttga tatccgtcgt ggagctgtag taccagcgcc
     2821 tgccgtcaaa tacccgaatg aatgcacccc tattattgcg gatcttctgt tcctgcaaag
     2881 tcagtttctt gaagctgatt ttggtatcaa aagtctcttc gatgcgaaca tcaacataca
     2941 accccttggg aaacttatac atcacgcgcc tcctttttgc tcctcggcat tagaatattc
     3001 aactagatgt ttaactattt ccataacgct atcatacagg atagtatatg ttctgcaagc
     3061 aataaaatgg attataaagg gtccctcagc gggatctcac ttgccataac tgcgaggctt
     3121 cttatgctcc atcctctggc attaaacccg ctccctgatg catctttcgc aggcgatggc
     3181 aatatatgcc ttggaatgag cggatatggc aatggagaat cggaccgtcg gacataagga
     3241 tagatgccta cctgattctt agggcaatcg ttccgtatcc gaacacagtt gatgccttaa
     3301 cttccgatgt catacgggat agagcctgaa aagtcgcaaa acccgtccgc ccggcgttgt
     3361 gcaattcaaa ctccggatct tggcataggt tgctgatgaa gaacccgccg aacagaccca
     3421 agtttttcct tccgcacttt accacttccc atacttccag ccagactctg caggcttttt
     3481 tgacattacc gtcaagcaaa tatcgatagc cctcatcaac cagatcggca actcgctccc
     3541 ttcggacacc ctaatgccat gaccgcaaat acatatatcc cattatgcaa tgcttgagtc
     3601 tgctgtttat ctcttccggg cttggcttgc tgcccaaagt ctcat
//
LOCUS       DAOSAI010000263         3624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16298_length_3624_cov_2.917624, whole genome shotgun sequence.
ACCESSION   DAOSAI010000263 DAOSAI010000000
VERSION     DAOSAI010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16298_length_3624_cov_2.917624"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PLM83_08540"
     CDS             complement(<1..1288)
                     /locus_tag="PLM83_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71487.1"
                     /translation="MTVPGWGPVVIDLQRLELPQRVTLLTDHDGRTEAAVGWGVPTTD
                     AKTLVIEGTLTEATEAGQRVLALLKDNVPLQASVGVEPKQYRPLQPGERITVNGRTIT
                     IEEPITLIENGILREVSIVPMGADGSTMVTLVAGVNSMTDQAPLADTPSPPNTIEAKY
                     VQDLATACGDDFATFKQALTNHWPPEAAKAVAEARRRPSVPPRGGHAKGQPANSTAIL
                     AAAALKLLGKESLAEKELGAEALTLAASYHPRTALDLCNVACQMSLGYTPRGVNEIIR
                     ASFSTTDLPVALGLAANKVALDAYLQAPMTWKSFARVVSAKDFKDHTGIRLTGDLKLE
                     ELPPTGEIKHGSLNEETVTYRVATYAKMLVLDRHHIVNDDIAILDQIPTIFGRASARK
                     VTDLVYSVLLSNPAGFFSTGHGNLLTGSSSALSVASL"
     gene            complement(2830..>3624)
                     /locus_tag="PLM83_08545"
     CDS             complement(2830..>3624)
                     /locus_tag="PLM83_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71488.1"
                     /translation="ELPSAARSALDTYRLLLPQIDENRWKMETWISTGYRIREWTAFL
                     QKISMIMSQEGFSPSDDKFWKQAQPFNSQIVSQSVPTENMAVVYMRDTRSLRPLKEKL
                     EQSGFYVRDTDDTITRLESLSRSTLIRLGFVTAFFLLIFCGFMLALGAIQAMRVEAQL
                     HQIALLKAIGMSEDRLAWLFLVQSFVIWLVASAIATAVSFPLGRFVVAPALIPESTRY
                     ANVAFHFDFMMAFSITFGVAIFWIAMTLYTTRRAIRYSPARSLQAL"
BASE COUNT          853 a          911 c         1054 g          806 t
ORIGIN      
        1 caagactggc aacactcagg gcagagcttg agccggtgag caggtttcca tgcccagtgc
       61 tgaagaaccc cgcgggattg ctgaggagca cagaatagac caggtcagtc accttgcggg
      121 ctgaagcacg gccaaagatc gtgggaatct ggtccagaat tgcaatgtcg tcgttaacga
      181 tgtggtgacg atccaggact agcattttgg cgtaggtcgc cacgcggtag gtgacagttt
      241 cttcgttcag gctgccatgc ttgatctcac ccgtgggggg cagttcctcc agcttcaggt
      301 cgccggtcag gcgaatgccc gtatgatcct tgaagtcctt ggctgaaacg acacgggcga
      361 aggatttcca ggtcatggga gcctgcaggt aagcatccag ggcgaccttg ttggcagcca
      421 atcccaaggc aacgggcaag tcggtggttg agaatgaggc ccggatgatt tcgttgacgc
      481 ctcggggcgt gtaccccagg ctcatttggc aggccacgtt acagaggtcc aaggctgttc
      541 gcgggtgata actggcagct agcgttaggg cctccgcccc aagctctttt tcggctaacg
      601 attccttgcc aagcagtttt agggcagcag cggcaaggat cgcggtagaa ttggcgggct
      661 gccctttggc gtggccgcct cggggcggga cggagggccg gcgccgggct tcggcaacgg
      721 ctttggcggc ctcaggcggc cagtggttgg tgagggcctg cttgaaggtg gcgaaatcgt
      781 cgccgcaagc agtggccagg tcttgaacgt acttcgcttc aatcgtgttg ggcggggatg
      841 gggtatcggc gaggggggct tggtccgtca tgctattcac tcctgcgact aaggtgacca
      901 ttgtgcttcc gtccgctccc atcgggacaa ttgaaacttc ccgaaggatt ccgttttcaa
      961 tcagggtgat tggttcttca atggtgatcg ttcgtccatt gacggtgatt ctctcaccag
     1021 gttgtaatgg gcgatactgt ttgggttcga cgccgacgct ggcctgcagg gggacgttat
     1081 ccttcagtaa ggcgagaacc cgttggcccg cctcggtggc ttcggtcagt gttccctcaa
     1141 tcaccagggt ttttgcatcg gttgtcggta caccccagcc gacggctgcc tccgtgcggc
     1201 cgtcgtggtc agtcagtagg gtgacccgct gcggcaattc caggcgttgt aggtcaatta
     1261 ccacgggacc ccagcccggc acagtcatga cacctccggt gtaggcaatc acgcgaacct
     1321 tggcgggctt cttcttatcc gcttgagcct caagagttag gcgggtgttt agtcgtagat
     1381 agtctgtcgt ggttaccatt gagtcccgat tagttcgatc cactattggc tagttggagg
     1441 attcgcttaa ctggaacggg attgttctcc atcaagtggc caacgagtgt tggtttagcc
     1501 cgccctgttt gaacacacca agcgaaaaac ttgtcggcca gatcgtcatt gaaaagggta
     1561 ttttctgcgc aaatcggttc tccatctgcc cagcatccat cggtgacggt atccagccaa
     1621 gcctgaatgt cttggagggt gacgatcagg ccccgaccct cagcttcacc aacgagggaa
     1681 ggccccggca tgggccaacg ggcggcaatc agtgtcttgg cttgggtttc ggtcaagcct
     1741 aatcgcttca gggccaagag taggacggct tcgtattcgg tattgctcag gaatcgcatg
     1801 ttgtatctcc catgcttgta ttatggcaga cttccgtttc ttgacagtcc tccggcgtgg
     1861 ggacaacttt tttgccgccc caaccgcagc aagggcacct cagatagcaa acgcgggaga
     1921 ggccggcctt cttgctggtg cggacaatga cttggccccg gcagtcgaac cagattcgcc
     1981 aagcaaattc ggcgagtagt cttgccaata gtgggtgcga cataaggaag cctcctaaag
     2041 ggttcttgga acggtccagt ttcacactgg tacttagtac cccatccctc gccggaatgg
     2101 gtgttaccaa aggctgtatg atgtggaaaa ctttcggtac tccaagtctt caacgttctt
     2161 cagaacacga acgttttccc agtggataag gagtataccg ggaagttgac ccaggaaata
     2221 ccttgtatct gtagtcgcat gatcagaaag cttgagggac attcgtaaaa ggtccggtag
     2281 gcaaatatcc tcttgatggc ggacgtcaat ggtctagcct ggacggatga agagatcgga
     2341 gaagcctacc accgtcgggt aaaaacggtg gagaatgcct gtaagcgcga tgtgaataag
     2401 gactcgagat ggccccggac gacaagcacc ggaaaacgct actaattgcc cgaaagccag
     2461 acgggaacag aggggtaaag ctcctcgccg cgagtcccag ttggcgtcca gaagggtttg
     2521 cccgccggac attgcgactc ctgagggaga atctggtaga gccgcaacgc atagaatcga
     2581 tctttaggga aacaatccgc aagacgctgc aaggaccctc agataggcaa gctcaccacg
     2641 accgacatac agatcgagtt ggcgaggccg tacccaggat taaggttgga tgatgcgcca
     2701 gcaaaatacg agtcaggatt tgaagcaggt gtcgcgcgag tcgaacgcga ttggatactt
     2761 tgtgattttc attacgtctg cagtccccag gaagcacaat ggttaataaa agtaggctaa
     2821 aggaagcctc tacaaagcct gaagagaacg ggcaggggag taccggatcg ctctcctagt
     2881 agtgtaaaga gtcatggcga tccagaagat agccacgcca aaggtgatcg agaaagccat
     2941 cataaaatcg aagtggaatg ccacgtttgc atagcgcgtg gattccggaa tcagtgccgg
     3001 agcgactacg aagcgtccaa gaggaaaact cactgcggtc gcaatcgcgc tcgctactaa
     3061 ccagataaca aatgattgga ccagaaatag ccacgcaagg cggtcctccg acatcccgat
     3121 ggccttaaga agggcaatct ggtgtagttg cgcttccact cgcatcgctt gaatggctcc
     3181 gagtgcgagc atgaacccgc aaaaaattag aagaaagaag gcagtgacaa agcccaaacg
     3241 gattaaagtc gagcgactaa gggattccag ccgagtaata gtgtcgtcag tatcacgaac
     3301 gtagaatccc gattgttcca atttctcctt caaaggacga agagaacgag tatcgcgcat
     3361 gtaaacaacg gccatattct ctgtaggaac tgattgagaa acaatttggc tgttaaatgg
     3421 ctgggcctgt ttccaaaact tatcatctga aggtgaaaac ccctcctggc tcattatcat
     3481 actaattttc tgtaaaaaag ctgtccattc gcgtattcga taaccagtag aaatccacgt
     3541 ttccatcttc catcggtttt cgtcaatctg tgggagtagg agacggtaag tatcgagtgc
     3601 tgagcgagca gcggacggca attc
//
LOCUS       DAOSAI010000264         3611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16382_length_3611_cov_4.640326, whole genome shotgun sequence.
ACCESSION   DAOSAI010000264 DAOSAI010000000
VERSION     DAOSAI010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16382_length_3611_cov_4.640326"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1070)
                     /locus_tag="PLM83_08550"
     CDS             complement(96..1070)
                     /locus_tag="PLM83_08550"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOK71489.1"
                     /translation="MERFFISTSERPPVTAQVFALGTFDGVHLGHMELIREAISLARD
                     KGEGPRSGVYTFDVHPLAVLDPSKAPPLLTSLDDRLKLIESAGIDVAVVQSFDAELAD
                     MSPESFVADILWRKLRARHVVVGFNYTFGRYGNAGASELEEMCARYGIGTTVVPPVTC
                     EGITVASTAVRTALAEGDVVLASRLLGRPYFLRGIVERGRGVGKSIGVPTANLRLPNG
                     HAIPAHGVYAARASWGDGQCAMAVCNIGVRPTFRDEGRGTTCEVHLVDVSQALYGEML
                     MVEFVARIRDEKRFASPSHLKAQIASDVEATRALIGNWSLHPISSVLE"
     gene            complement(1091..2014)
                     /gene="truB"
                     /locus_tag="PLM83_08555"
     CDS             complement(1091..2014)
                     /gene="truB"
                     /locus_tag="PLM83_08555"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOK71490.1"
                     /translation="MDGIIIINKPKGMTSHDVVVEVRRITGERSCGHTGTLDPDATGV
                     LVVCMGRATRLSRFMMETPKEYEAVIVFGISTDTGDASGKVVAVSEDVKVPRERVFEA
                     FQRFVGDIEQIPPMVSAVRHRGRRLYELAREGITVEREPRRVTIYEIEPASLEDWPEV
                     VRLGSQGSMRVVCSKGTYIRTLCEDICASIGIEGHMGALERTASSGFRLSEACTLNEL
                     RQAAAYGEFDNLVRPIVEGVGHMPKCVVSEDEASRIKHGTQIEWDPNRAEPVCGIGDY
                     IELLGADGALVAVGEVRLMDGRALIQPRVVL"
     gene            complement(2002..2988)
                     /locus_tag="PLM83_08560"
     CDS             complement(2002..2988)
                     /locus_tag="PLM83_08560"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOK71491.1"
                     /translation="MSAPEELLQAIVDAKSILITTHVAPDGDAIGSALALKIALDRMG
                     KGPRVGLSDPVPEFLRFLPEADSIETAECVGGPFDLALVLDCGSLDRVGKCENAVVSS
                     RKVANVDHHTTNTHFADINWVDSEAAATAQMAAGIIRDLGVEFDPDIAACLFAGLSTD
                     TGSFRYSNTSADTFRVAAALVDAGARPWEIAQNIYDTKPYKSLSALAEAILGIQFSSG
                     GLIASMEITAEMMERHGITESETEGFIGYARSIEGVEVAVAFREVEDGLIRIGFRSKE
                     KVDVAEIAAEYGGGGHVRASGCTVPGPLAEVKSNVISRLNRALAEAGYTWTE"
     gene            complement(2985..3362)
                     /gene="rbfA"
                     /locus_tag="PLM83_08565"
     CDS             complement(2985..3362)
                     /gene="rbfA"
                     /locus_tag="PLM83_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOK71492.1"
                     /translation="MSSYRPDRVKAAIRQEVSNILQRDLKDPRLGFATVTDVDISGDL
                     QHVKIYVSILGEEESRTETMKALESAKGYIRSEIGRRVPLRLTPEISFEYDESIERGA
                     RILKLIEEVQSGRNDEESGDMPK"
     gene            complement(3395..>3611)
                     /locus_tag="PLM83_08570"
     CDS             complement(3395..>3611)
                     /locus_tag="PLM83_08570"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOK71493.1"
                     /translation="RVIQGMMARIRHRFNVAVAEVDCQDIWNKAQIGIVCVSNDSGHA
                     HSILEAVARMIDLSPDVVLYSYEIEIL"
BASE COUNT          847 a         1120 c          828 g          816 t
ORIGIN      
        1 cctgtttttc gtacgccctt gccaagaatg ccgtcggagg atcggcaatc ctagccaagg
       61 gtcaagcaag cccgaatgat gcacgcttgc ggagttcatt ctagcacaga tgatattggg
      121 tgtaaactcc aattgcctat gagagctcgg gtagcctcga catccgacgc gatctgcgcc
      181 tttaggtgag acgggctggc aaaccgcttc tcgtcacgaa tcctagccac aaattccacc
      241 attagcattt ccccgtacag cgcctgcgaa acatcgacca aatgaacctc gcacgtggtc
      301 cctcgaccct cgtctctgaa tgtgggccga acgcctatgt tgcacacagc cattgcacac
      361 tggccatctc cccaagatgc tctggccgca taaactccat gagctggaat agcatgccca
      421 ttcggaagcc tgaggttggc agtgggcaca cctatcgatt tccccacacc acggccgcgt
      481 tcgacaatcc ctctcagaaa ataaggcctg ccaagcagcc tgctagcgag cactacgtct
      541 ccctcggcga gcgctgttcg aacagccgta ctcgccaccg taatgccttc gcaagtcaca
      601 gggggaacca cagttgtgcc aattccatat ctggcgcaca tttcctcaag ctcgctggca
      661 ccggcgttgc cataacgacc gaaagtgtag ttgaatccca caacgacatg ccgagcgcgg
      721 agtttgcgcc acagaatatc tgcaacaaag gactccggcg acatgtctgc cagttccgca
      781 tcgaaagact ggacaaccgc gacatctatg cctgcgcttt caataagctt cagcctgtca
      841 tctaatgatg tgagcagcgg cggcgccttc gaggggtcaa gcactgcaag aggatgaaca
      901 tcgaaagtgt atacgccgct tcttggccct tcgcctttgt cgcgcgcaag cgagatggcc
      961 tcgcggatca gctccatatg gcccagatgg actccgtcaa atgtccctaa ggcaaacacc
     1021 tgagccgtaa caggaggtct ttctgatgtg gaaatgaaga agcgctccat atgacacaac
     1081 acctcagaaa ctacaacaca actcttggtt ggataagtgc tcgtccatcc atcagacgaa
     1141 cctcgcccac ggcaacaagg gccccatcag caccgagcag ctcgatatag tcccctattc
     1201 cgcagacagg ctctgcccgg ttcggatccc actctatctg agtcccgtgc ttgatacgcg
     1261 aagcttcatc ttctgaaacc acgcatttcg gcatgtggcc cacgccttca acgattggcc
     1321 tgacaagatt atcaaactcg ccatacgcag ctgcctggcg aagctcgttg agggtgcatg
     1381 cttccgacag ccgaaatccg gacgaggccg tacgttcaag cgcccccatg tgcccttcta
     1441 tcccaatgct cgcacaaatg tcctcgcaca gcgttcttat gtaagtccct ttcgaacaga
     1501 ccacgcgcat agagccttgc gagccaagcc tcacaacttc cggccaatct tccaaagatg
     1561 ctggctctat ttcgtatatc gttactctcc ttggttccct ctcaacagtt atcccttcac
     1621 gagcgagctc ataaaggcgc cgcccacggt gacgcactgc cgaaaccatg ggaggaatct
     1681 gctcaatatc gcctacaaag cgctggaaag cctcgaatac tctttcccgc ggaaccttga
     1741 cgtcctcact tacagcaacg accttacctg aagcgtcccc cgtgtcagta gatatgccga
     1801 agacaataac cgcctcgtat tctttgggcg tctccatcat gaaccttgaa agccgggtag
     1861 cccttcccat acaaacaacg aggaccccgg tggcatcagg gtccaacgtt ccagtgtggc
     1921 cgcatgagcg ctcgccggtg atccgcctca cttcaacaac aacatcatgt gatgtcatgc
     1981 ctttcggctt gttgatgatg attattccgt ccaagtatac ccagcctccg ccaatgcccg
     2041 gttcagcctg gaaatcacat tcgatttcac ttctgcaagg ggacctggaa cagtgcaccc
     2101 tgaagcgcgc acatgccctc cgccgccgta ttccgcagca atctcggcca cgtccacctt
     2161 ctctttcgag cgaaatccga ttctgatcag tccatcctct acctctcgaa aagcaacagc
     2221 cacttctacg ccctcaattg atcgagcata gccgatgaat ccttcggtct cactctccgt
     2281 aatgccatgg cgttccatca tctctgctgt gatctccatg gacgcgatca ggcctccact
     2341 cgagaactga atcccgagga ttgcctcggc caaagccgaa agggacttat agggtttggt
     2401 gtcatatatg ttctgcgcaa tctcccatgg cctggccccc gcgtctacaa gggcagcagc
     2461 tacacgaaac gtatccgcgg aagtattcga atacctgaat gaacccgtat cagtggacaa
     2521 ccctgcgaaa aggcaggccg caatatccgg gtcgaactcc accccaagat cacggataat
     2581 accagctgcc atctgagctg tggcagctgc ttccgaatct acccagttga tatcggcgaa
     2641 atgagtgttc gtggtgtggt ggtctacatt agcaaccttc ctgctgctta ctacggcgtt
     2701 ttcacacttt cccacccgat ccaacgagcc gcagtcgagc actaaagcca agtcgaacgg
     2761 cccgcccaca cattccgccg tctctatcga gtcagcctcc ggcaaaaatc taagaaactc
     2821 gggaacggga tcagaaaggc caacccgcgg gccttttccc attctgtcca gcgctatctt
     2881 gagtgccagt gcagatccga tagcatcacc atcaggagcc acatgggtag tgatcaggat
     2941 ggactttgcg tccacaatgg cctgcaacag ttcttctgga gcgctcactt aggcatgtca
     3001 ccgctttcct catcgttccg tccgctctgg acctcctcga tcaatttgag tatgcgagct
     3061 cctctttcaa tggattcatc atactcgaaa ctaatttcgg gagttaagcg caacggcacg
     3121 cgacgcccga tctccgacct aatgtagccc tttgcgcttt ccagggcctt catggtttct
     3181 gtgcgggatt cttcttcgcc aagtatgctg acgtatatct tcacatgttg aaggtccccg
     3241 cttatgtcca catcagtcac agtcgcaaat cctagtctag gatccttcag atcgcgctga
     3301 agaatattgg aaacctcttg acgtattgcg gctttgacgc gatcaggccg atagctcgac
     3361 atcggacagc acctccagag agtcctgcaa gaactcagag tatttcaatt tcgtacgagt
     3421 ataacacgac atccggagac agatcgatca ttctggcaac tgcttccaaa atgctgtggg
     3481 catgccctga atcgttgctc acgcagacga tgcctatctg cgccttattc catatatcct
     3541 ggcagtctac ttcagcaacg gctacattga accgatgtct gattcgggcc atcatcccct
     3601 ggattacacg t
//
LOCUS       DAOSAI010000265         3606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16406_length_3606_cov_3.304140, whole genome shotgun sequence.
ACCESSION   DAOSAI010000265 DAOSAI010000000
VERSION     DAOSAI010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16406_length_3606_cov_3.304140"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PLM83_08575"
     CDS             <1..967
                     /locus_tag="PLM83_08575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOK71494.1"
                     /translation="RLGLMHRYVALRKKALKLDDVHMYDIYAPIVPDIEWTVTYEEAK
                     KAVCEGLAPLGDEYVSILGAGLESGWVDVVESRGKTSGAYSWGEYGTHPYVLLNWQDN
                     LDNMFTLAHEMGHAMHSHYSWTTQPYVYSDYSIFLAEIASTANEVLLMEHLLSLTEDQ
                     VRRMYLLNHLLEQFRGTLYRQTMFAEFEKIAHERYEAGQALTPDALNDIYLQLNRDYY
                     GDEIVLDDRIALEWARIPHFYSAFYVYQYATGFSAAVALAESILEHGDPARDRYIEFL
                     RGGRSKYPLDLLRDAGVDMTSPDPIHRALDVFEKSLEEMETYFRG"
     gene            1063..3003
                     /gene="pulA"
                     /locus_tag="PLM83_08580"
     CDS             1063..3003
                     /gene="pulA"
                     /locus_tag="PLM83_08580"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOK71495.1"
                     /translation="MGRETSSVVAVGGQEVFELYYPGDDLGAVYSKESTTFRVFAPTA
                     LGVSVALYSSTEATVADAIEMAPDCDGTWRATVPGDLEGVCYTYRVDLGGVVNEAVDP
                     YARALTANGVRGVVVDLDMTDPEGWENDVRPPFGAATDAIIYEVHVRDFTSSPDSGVR
                     YPGKYLGMADRGSRGPAGVCTGLDHLAELGVTHVQLMPIQDFASVDELDKDGYNWGYD
                     PYHFFVPEGSYSIAPECGIARIIEVKKMIQAMHSAGLRVIMDVVYNHTWSVEDSPLHM
                     IVPSYYHRADCAGRYTNGSGCGNELATERPMVRKLILDSLRYWVEEYHVDGFRFDLMA
                     LMDYETAREIELRLHAIEPSLLLYGEPWAGGLSGLSSHRMFTKGRQRGLKIGFFNDGF
                     RNAIKGDNDGVRRGFATGAGGCGRAIERGVVGGIPYSADIADFATEPSECINYVSCHD
                     NLTLWDKIARSAPLDSEEDRIKMDLLAQAIVMTSQGIPFMEGGAEMLRTKGGHCNSYR
                     AGDAINRFDWVRKLDYAEVFDYHRGLIQLRRAHPAFRMCSAEAIRRSLRFLPAPADNV
                     VVFMLDGASCGDEWDKIVVVYNPNRQDVRIDLPIEGEWKVVVGGASAGVEPLDPDLFG
                     LRTYEGGAAVVAKAISTTVLHT"
     gene            3037..>3606
                     /locus_tag="PLM83_08585"
     CDS             3037..>3606
                     /locus_tag="PLM83_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOK71496.1"
                     /translation="MPANLTPQYMEAEEAYKRATTADEKIAAVEEMLRTIPKHKGTEK
                     LQADLRKRLARLRAEGRELRKKGKADPYCVQREGAGQVFVVGPPNSGKSALVGALTNA
                     KVVVAPYPYATPLPVPGMMKYENIQIQLVDCPPISASNCPPALTASMRTGDGVMIVLD
                     ASSDSCLDDLDEQLALLRERKVIEDAKRFL"
BASE COUNT          840 a          834 c         1102 g          830 t
ORIGIN      
        1 caggctcgga ttgatgcata ggtacgtcgc cctgcgaaag aaggctctga agctcgacga
       61 tgtgcacatg tatgatatat atgcacctat agttccggat atcgaatgga cagtcacata
      121 cgaggaagca aagaaggctg tctgtgaagg ccttgcgccg ctgggcgatg aatacgtgag
      181 catccttggg gcaggtctcg agtcggggtg ggtggatgtc gtcgaaagca gggggaagac
      241 atctggggct tattcctggg gcgagtatgg aacccatccg tatgtcctct tgaactggca
      301 agacaacttg gacaacatgt ttactttagc ccatgagatg ggccatgcca tgcattcaca
      361 ttattcctgg acaacacagc cctacgtata ctctgattac tccatattcc tggccgaaat
      421 cgcctctaca gctaatgagg tgttgcttat ggaacatctg ctcagcctga cggaggatca
      481 agtgcgcaga atgtacttgc tcaatcacct ccttgagcag ttcaggggca cactttacag
      541 gcagacgatg tttgctgagt tcgagaagat agcccatgag aggtatgagg caggccaagc
      601 cctgaccccg gatgcgctca acgacatcta tctgcagctc aacagggatt actatggaga
      661 cgagatcgtg ctcgatgaca ggatcgccct cgagtgggcg cgaatcccgc atttttacag
      721 tgctttctat gtataccagt atgctacagg gttttcggca gcggttgcat tggccgaaag
      781 tatactggag cacggagacc cggcgaggga tcgatacatc gagtttttga gaggtggaag
      841 gtcgaagtat cccttggatc ttctgcgtga cgctggagtg gacatgacat cgccggatcc
      901 tattcaccga gcgctggacg tgttcgagaa atctctggaa gagatggaaa cgtattttag
      961 aggatagcaa cgaaatatgc catgcctggg tggacactga agaacgagat actgtattat
     1021 ataagcagca gctagaatgc agtttcgtgg atggtgaaat gtatgggaag ggagacatct
     1081 tccgtcgtcg ccgtaggcgg ccaggaagtg ttcgagctat attacccagg cgacgatctc
     1141 ggggctgttt attccaaaga atcaacgacg ttccgcgtgt ttgcccctac agctctcggc
     1201 gtgtcggtgg ctctttactc gtcgaccgag gcgactgtcg ctgatgcgat cgagatggcg
     1261 ccggactgtg acgggacttg gcgggccact gtccccggcg accttgaggg agtttgctat
     1321 acgtaccgcg tcgacctagg gggagtggtc aacgaggccg tcgatccata tgctcgtgca
     1381 ttgactgcaa acggggttcg cggcgtcgtt gtggatctgg atatgactga cccagaaggc
     1441 tgggagaatg atgtacggcc gccctttggg gcagcgacag acgcgatcat atacgaagtg
     1501 cacgtacggg atttcacgag ttctcctgat tcgggggtac gttatcccgg aaaatacttg
     1561 ggcatggctg ataggggttc tagaggtcct gccggagttt gcaccggcct cgatcaccta
     1621 gcggagctgg gagtcaccca tgtccagctg atgcccatac aggattttgc ttccgtggac
     1681 gagctggaca aggatggcta taattggggc tatgatccgt accatttctt tgttcccgag
     1741 gggtcgtact ctattgcgcc cgagtgcgga attgccagaa tcatagaagt caagaagatg
     1801 attcaagcta tgcattcggc gggcctacgt gtgattatgg atgtcgtgta caatcacacg
     1861 tggtctgtgg aagactcgcc gctccatatg attgtgccgt cttattatca ccgcgctgac
     1921 tgcgcgggca ggtacacaaa tggatcagga tgcgggaacg agttggcaac ggagaggccg
     1981 atggttcgaa agttgatcct cgattctttg cgatactggg tcgaagagta ccatgtagac
     2041 ggcttcaggt tcgatctcat ggcgctgatg gattacgaga cagcgaggga gattgagttg
     2101 aggttgcatg caatcgagcc gtcgctgttg ctttacgggg aaccctgggc cggtgggctt
     2161 tccgggctgt catctcacag aatgttcaca aaaggcagac aacgtggatt gaagataggt
     2221 tttttcaacg atggattcag aaatgccata aaaggcgata atgacggagt caggcgtggg
     2281 tttgcaactg gcgctggtgg atgtggacgt gcaattgaga ggggcgtagt tgggggaata
     2341 ccttactcgg ccgacatagc tgattttgcg acagagccga gcgagtgcat aaactacgta
     2401 tcgtgccatg acaacctcac attgtgggac aagattgccc ggtcggcgcc gttagattcc
     2461 gaagaggaca gaatcaagat ggacttgttg gcgcaggcga ttgtaatgac gtctcagggt
     2521 attccattta tggagggagg cgctgagatg ctcaggacaa aaggcggaca ctgcaatagc
     2581 taccgtgcgg gcgatgcgat taaccgcttc gattgggtgc ggaagttgga ttatgcggag
     2641 gttttcgact accacagagg gctgatacag cttcgtcggg cacaccccgc attccgaatg
     2701 tgctctgcag aggcaatcag gcgttcactc agattcctgc cggctcctgc agataatgtt
     2761 gtggtgttca tgttggatgg cgcttcgtgc ggagacgagt gggataagat tgtcgtcgtt
     2821 tacaacccca acaggcaaga tgttaggata gatctgccga tcgagggcga gtggaaggtt
     2881 gttgtcgggg gagcttcggc cggagtggag ccgctcgacc ctgatctgtt cggactgcga
     2941 acctatgaag gaggggctgc tgtggtggcg aaagccatat caacaacggt cctacatacg
     3001 tagtgcagcc gggcccctgg aaaaggagga gcacgcatgc ccgccaacct caccccgcag
     3061 tacatggagg cggaggaggc gtacaagcgc gcaaccaccg cagatgaaaa gatagccgct
     3121 gtcgaagaga tgctcaggac gattccaaag cacaaaggca ctgagaagtt gcaagctgat
     3181 ctcaggaaac gtcttgctag gcttcgggcg gaaggccggg aattgcgaaa aaaaggcaag
     3241 gccgatccct actgtgtgca aagagaaggg gcgggtcaag tattcgttgt tggcccgcct
     3301 aattccggca agtcggcgct ggttggggca cttaccaatg ctaaagtagt ggtggcgccc
     3361 tatccttatg ccacacctct gcctgtcccg ggaatgatga aatacgagaa catacagatc
     3421 cagttggtgg attgcccgcc tatctctgct tccaattgtc cccctgcctt gactgcctct
     3481 atgcggacgg gtgacggggt tatgatcgtt ttggatgcca gttctgattc atgcctcgat
     3541 gatctcgacg agcagcttgc gttgttacgg gaacgaaaag tgattgaaga tgcgaaacgc
     3601 ttccta
//
LOCUS       DAOSAI010000266         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16495_length_3591_cov_3.870758, whole genome shotgun sequence.
ACCESSION   DAOSAI010000266 DAOSAI010000000
VERSION     DAOSAI010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16495_length_3591_cov_3.870758"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLM83_08590"
     CDS             complement(<1..183)
                     /locus_tag="PLM83_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOK71497.1"
                     /translation="MFIIAGLGNPGTQYECSRHNIGFMVADRLALELGAGKFRRASHC
                     LSLVAKGTIAGTDTLIV"
     gene            complement(205..1152)
                     /locus_tag="PLM83_08595"
     CDS             complement(205..1152)
                     /locus_tag="PLM83_08595"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOK71498.1"
                     /translation="MDSCHRKLKIFACNSNPELSKAIAANLETDIVKAEVGRFSDGEI
                     SVQIFESIRGADVFVIQSLCAPVHDNIMELLIVIDALKRASARSITAVVPYYGYARQD
                     RKSQPREPITSKLLANLITAAGASRVLTIDLHAPQIQGFFDIPVDHLKAAPILAQHLE
                     SRGLKGCVIVSPDVGGVTRARGIAEHLDSTLAIVDKRRPRPNVSEVMNIIGDVRGKPA
                     VIVDDLVDTAGTLCQAADALIEAGATEVRACCTHGVLSGPAIERLANSQISELVITDT
                     IPLSKEKQIDKISILSVAPLFAEAIRRIYLELSVSILFD"
     gene            complement(1155..2498)
                     /gene="glmU"
                     /locus_tag="PLM83_08600"
     CDS             complement(1155..2498)
                     /gene="glmU"
                     /locus_tag="PLM83_08600"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOK71499.1"
                     /translation="MASFVGLVLAAGHGKRMKSALPKPLHPVAGIPMIDHVVGSLVRS
                     GASRVVVVVGHGGELIEEHLAGRAEIVWQREQLGTGHAVLAAGPAFDGYDGNILIVPA
                     DVPLLTSESLQQLVEEQSGSASDATILSMCVDDPTDYGRVVRDSKGNVAAIVEHCDAT
                     EEIRAIREVNTGVFCFDSRTLFNALPRISTDNKQGEYYLTDVIAIMISQGLSVRAVAL
                     KDASEGLGINSRVELSKAEQIMGDRKRREVMESGVTLIDPATTFIDASVEIESDTVIM
                     PFTIIAGNSKIGGSCNIGPYVYIRDSEVGSGCNIGPFSYLRPGTSLADRVKVGDFVEV
                     KKSTVGEGSKIPHLSYIGDTVMGSHVNIGAGTITCNYDGETKHTTIIEDGAFVGANSN
                     LVAPVKVGSDAYIATGSTVTSHVPAGALAIARARQENKDGWVAKRRAARLSKEEK"
     gene            2688..3032
                     /locus_tag="PLM83_08605"
     CDS             2688..3032
                     /locus_tag="PLM83_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HOK71500.1"
                     /translation="MPKARKSNRTLVPQARQALDQMKYEVANELNIPTHLIEGDYWGN
                     LSARDCGSVGGNMVRRMIEAAQASLAATTMAGVKQGFQEVIPAQNFTAQEGNLSGPST
                     FPTSGNLPGTSF"
     gene            complement(3367..3440)
                     /locus_tag="PLM83_08610"
     tRNA            complement(3367..3440)
                     /locus_tag="PLM83_08610"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3406..3408),aa:Gln,seq:ttg)
BASE COUNT          877 a         1034 c          855 g          825 t
ORIGIN      
        1 cacgattagc gtatccgtcc ctgcaatcgt tccctttgca acaagactga gacaatgcga
       61 agcccgccgg aacttgccgg cgcccagctc caatgcgaga cggtcggcaa ccatgaagcc
      121 aatgttatgc ctggagcact catattgggt tccagggttt ccaaggcctg ctataatgaa
      181 catgtcacta cccgctgtaa aagactaatc aaatagtatg cttaccgaca gctcaagata
      241 gattctcctg atcgcctccg caaaaagcgg ggccaccgat agaatcgata tcttgtcgat
      301 ctgtttctct ttgctcaaag gaatagtatc ggtaataacc agttccgaaa tctgcgaatt
      361 ggcaagccgc tcaattgccg gccccgaaag cactccatgg gtgcaacatg cccttacttc
      421 agtcgccccc gcctcgataa gtgcgtccgc cgcctgacaa agggtgcccg cagtatcaac
      481 gaggtcatcg acgatcaccg ccggcttgcc gcgaacatcg ccgattatgt tcataacctc
      541 cgacacgtta ggccgcgggc gacgcttgtc cacaatggcc aacgtgctgt caaggtgctc
      601 agcaattccc ctggcgcgag ttaccccgcc cacatcggga gatacgatca cgcaaccctt
      661 caagcccctc gattccagat gctgcgcgag gataggggct gccttgagat ggtcgacagg
      721 tatatcgaag aatccctgga tttggggcgc atgcaagtcg atagtgagaa cccgggaagc
      781 ccctgcagca gtgatcaagt tggccagcag tttcgatgta atgggctctc tgggctgaga
      841 tttcctgtcc tgcctggcat agccgtaata cgggacaaca gcagtgatag acctggcaga
      901 tgcgcgcttt agcgcatcga tgacgatgag aagttccatg atgttgtcgt gaacgggagc
      961 acacaaagac tgtatgacaa agacgtctgc gcctcttatg ctctcgaata tttgtacact
     1021 gatttcaccg tcactaaacc tgcccacttc agctttgaca atgtctgttt cgagattggc
     1081 agctatcgcc ttcgatagct cagggttgga attgcacgcg aagatcttca actttcgatg
     1141 acatgagtcc atagctactt ctcctccttg gataatcgag ccgcccttcg ctttgcgacc
     1201 catccgtcct tgttctcctg cctcgctcgc gctatggcca gagcccctgc tggaacatgg
     1261 gaagtaactg tggacccggt cgcaatgtat gcgtccgagc caacctttac cggagcaacc
     1321 agattagagt tcgcccccac aaaagcgccg tcctctatga tcgtagtgtg tttggtttct
     1381 ccgtcgtagt tgcatgtaat tgtcccagcc cctatgttta cgtgggagcc catgaccgtg
     1441 tctcctatat aactaaggtg aggaatcttg cttccctcgc ctactgtgct cttcttcacc
     1501 tcaacaaagt cgccaacctt gacgcggtcg gcaagtgatg tgcccgggcg cagataggaa
     1561 aatgggccaa tattgcatcc gctgcccact tctgaatcgc gaatgtaaac atacgggccg
     1621 atattgcacg atcccccaat cttgctgttc ccggctatga ttgtaaatgg cataatgacc
     1681 gtgtcggact ctatctccac acttgcatcg atgaacgtgg ttgccggatc gatcagagta
     1741 acccctgatt ccatcacctc gcgtcgcttg cgatcgccca tgatctgctc ggctttggaa
     1801 agctcgacgc gggaattgat tcccagtccc tctgatgcat ccttaagggc aaccgctctc
     1861 acggacagcc cttgggagat cataatcgca attacgtctg tcaagtaata ctcgccctgt
     1921 ttgttatctg ttgatatacg cggaagcgcg ttgaaaagtg tcctactgtc aaaacaaaaa
     1981 acgccggtat tgacctcgcg aatagcgcgt atctcctccg tcgcatcgca gtgctctact
     2041 atggcggcga cgttcccttt cgagtccctg accaccctcc cgtaatctgt agggtcatca
     2101 acgcacatcg aaaggattgt ggcgtcgctt gcgctgccag actgctcttc tactagttgt
     2161 tggaggcttt cggaagtaag cagcggcaca tctgcaggta cgatgagaat attgccatca
     2221 taaccatcaa aagccgggcc cgccgctaag acagcatggc ccgtgcccaa ttgctcgcgc
     2281 tgccagacta tttctgcgcg cccagcaaga tgctcttcaa tcaattcgcc accatgtcca
     2341 acaacaacta ctacacgcga tgctccggag cgaaccaacg agcccacgac gtggtcaatc
     2401 atgggtatgc cggcaactgg atgaagtggt ttgggaagcg ctgacttcat ccgcttgcca
     2461 tgccctgccg ctagtacaag gcccacgaaa gatgccatat tattcactcc gccaaattaa
     2521 tccaatgttg aaactggtct tcaagcactt tgagcatata ccaagcatac tgcttcgtca
     2581 acttttcacc atgctgccaa acagagtgcc gatcggcatc cgtttactaa catatccccg
     2641 cctagctcgg caaagaatgt actcagcatc gcaatggagg tgaaaacatg ccaaaggctc
     2701 gcaaaagcaa tagaacacta gtcccccaag cgcggcaagc actagatcag atgaaatatg
     2761 aggttgccaa cgaactcaac attccaacac atctcattga aggggattac tggggaaatc
     2821 tcagcgcccg cgattgtgga tctgtcggcg gaaacatggt tcggcggatg atcgaggcgg
     2881 cacaagcctc acttgcagca acaaccatgg ctggagttaa gcagggcttt caggaagtca
     2941 ttcctgcaca gaacttcacg gcacaggaag gcaacttgtc aggtccgtca acctttccga
     3001 cttcaggcaa cttacccgga acctcgttct agccgtcccc gagccccatc agtctgaaac
     3061 cgcatgcacc gcgacggtga atattcttca aaggcgacgg gcgacgttca cttgcgggcg
     3121 gggcaatcct cagccccgcc cgcccatagt ctatatccgg tgtacggcca ctttcttgca
     3181 tgagtcgccc tttcagtata ctatccccat cgccgtgtaa gaatattcga acttatatcc
     3241 ttttcgccga gggcggaatc ttgattcatg ggctctcctg gaacgtgaca accaccaaaa
     3301 acaaaaaaca gggcaatcgc cctgtcagaa agttatccgt cgcatataca gcttgcctat
     3361 atataatggc tggggcgcca ggattcgaac ctgggctgca ggatccaaag tcccgtgcct
     3421 tacctcttgg ctacgcccca tcctaaagca gtacatattt tagcataaca aaagccggtg
     3481 agcaaatatt cgacgagagg gagagtttcc atccttacac gcctcgaacg cctgacaaga
     3541 gaaatacgcc atctcaacga aaacacgata taaaaggtgg ctcatctcag g
//
LOCUS       DAOSAI010000267         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16589_length_3578_cov_3.002271, whole genome shotgun sequence.
ACCESSION   DAOSAI010000267 DAOSAI010000000
VERSION     DAOSAI010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16589_length_3578_cov_3.002271"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(464..1522)
                     /gene="ftsZ"
                     /locus_tag="PLM83_08615"
     CDS             complement(464..1522)
                     /gene="ftsZ"
                     /locus_tag="PLM83_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOK71501.1"
                     /translation="MFELELEVDQLANIKVVGVGGGGSNAINRMISAGLKGVEFVALN
                     TDAQALRMSNAPRKIQIGEKLTKGLGAGANPEIGKKAAEESRDLLAETLDGADMVFIT
                     CGMGGGTGTGAAPVIAEIAKEQGALTVGVVTKPFSFEGRRRMAQADHGTEELKDKVDT
                     LIVIPNDRLLQVAESQTSMLDAFRIADDVLRQGVQGISDLITVPGLINLDFADVRTIM
                     TDAGSALMGIGIASGENRAAEAARSATASPLLEASINGAKGVLMNITGSSNMGLFEVN
                     EAAEIVSEAADPDALVIFGAVIDDSMGDELKVTVIATGFDVQQAEQPLKARTDRVQIK
                     PLAGSDDLDIPPFLRRAR"
     gene            complement(1547..2764)
                     /gene="ftsA"
                     /locus_tag="PLM83_08620"
     CDS             complement(1547..2764)
                     /gene="ftsA"
                     /locus_tag="PLM83_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOK71502.1"
                     /translation="MGKNDIKVGLDIGSNQIHVVVCERDEDYNLNIIGVGSGPSAGLR
                     RGVIVDMDSAAAAIADAVAHAEEMSGCAAVSVLASASGTHVSSIASRGVVAIGRSDRE
                     VTQEDVDSVLEAARVVAIPPDREVVHVLPREFVIDGCSGIRKPVGMTGIRLEVDASII
                     TGSATAIQNIHRSARRIGLEVEHVILQPIAAAEAVLTLSEKEQGALVIDMGGATTDIA
                     VCIEGGVHHVASIPVGGNHVTNDIALVMRLPVGYSEGLKLNYGSALPDMVGENESCGA
                     DTNISRKKLCQIIEARITETMELVGDELDRARYRDLLPAGVVVTGGASVMQGVVEVAQ
                     EVLGLPARLGVPVGVYGAEEALDNPQYATAVGLVKSVWSESAVIPSADGRRPKSGGFA
                     SEVRQWFRDIFSS"
     gene            complement(2937..>3578)
                     /locus_tag="PLM83_08625"
     CDS             complement(2937..>3578)
                     /locus_tag="PLM83_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOK71503.1"
                     /translation="NSPYFLVRAVRVSGSNYLSDYEVLLIAGIPENTNMFLVPTGEIE
                     RKLLATPRIRTAKVYRILPDTISIAIEERSTAGYLPYGGYFIDVDEEGYAIGISEAIT
                     DPDVPLIVGVAPTWVEVGQPVNPKYEVSVGARVGAALLKQGIPNISEVDVSSCEDIII
                     RTSDGIKVFIGGARDMESQVRVLDSILASVREQEMSADYIDLRVEAKPVIRSR"
BASE COUNT          811 a         1130 c          787 g          850 t
ORIGIN      
        1 gaaggagcaa agaatccagg tatatgcgga tcatactcta cgccccctca ttgtatgatt
       61 gtatgcagtt gatccccgga ttcttctcac aatgtgaatg tagaagtcaa ggaaggctct
      121 gtgtgttgaa gtgtaacact gcgctccgtc gaaccgtgtc gggttgcgcc gacaggacaa
      181 acgaatgatt ccggtattac gcaaaactgc cttgcccacc atgcactgaa caagctgcag
      241 gcagggcagt tggacatctt gttcaattcg tctgaaatga tcatacagct ggatatcaga
      301 aggcctctac ttgaagccaa gccctatagc caaacgcaaa ttggcacggc ttcggaggcc
      361 cgtccgctcc cggctcctgt acagctgaac gcggggcgcc cctgcctcct ggcaaatcag
      421 cgccccgcta cacgaactct cctcatttca atctgctccg gttctacctt gcccgtctca
      481 ggaacggagg aatatccaga tcgtcgcttc cagcgagagg cttgatctgg actctatcag
      541 tcctggcctt cagaggctgc tccgcctgtt gaacatcgaa tccagtagcg ataacggtca
      601 ccttaagctc gtcccccatt gaatcatcaa tgactgcgcc aaagatgaca agcgcatccg
      661 ggtcggcagc ctccgaaact atctctgctg cctcgttcac ctcaaataag cccatatttg
      721 aagacccagt aatgttcatg agaacgccct tcgccccgtt tatggaagcc tccaaaagcg
      781 ggctggccgt ggcggagcga gcggcttctg cagcgcggtt ttctccgctt gcaatgccta
      841 tccccatcaa tgcagaaccc gcgtcagtca tgatagtcct tacgtccgca aaatcgaggt
      901 ttatcagtcc cggaacggtt atgaggtctg aaatcccctg cactccttgg cgtagcacgt
      961 cgtccgcaat cctgaacgca tcgagcatcg atgtctggct ctctgcaacc tgcaacaacc
     1021 tatcattggg tatgacaata agggtatcga ccttatcttt cagctcttcc gttccgtggt
     1081 ccgcctgcgc cattcgtcgc ctgccctcaa atgaaaacgg ttttgttaca acgcccactg
     1141 tgagagcgcc ttgttcctta gcgatctcgg caatcaccgg ggcagctccg gtacctgtgc
     1201 ccccgcccat tccacacgtg atgaacacca tgtctgcgcc atccagcgtt tctgcaagca
     1261 aatctcggct ctcttcagcc gccttcttac caatctcggg attagcgcca gctccaagcc
     1321 cttttgtaag cttttcgcct atctggatct tccggggcgc attagacatg cgcagggcct
     1381 gggcgtcagt atttaaggcc acgaattcga caccttttaa tcctgcactt atcatcctgt
     1441 tgatggcgtt actcccgccc ccgccaaccc caaccacttt gatgttggcc aactggtcaa
     1501 cctcaagctc tagttcaaac acctcgcatc ccccttgccg ctcgcctcac gaggaaaata
     1561 tatctcgaaa ccattgtcgc acttcgctag cgaaacctcc ggactttggc cgccggccgt
     1621 ctgcagaggg aatcaccgca gattcactcc aaacagattt gactaacccc accgcagtag
     1681 cgtattgagg gttgtccaac gcttcctcag ctccatatac ccctacagga actccgaggc
     1741 gcgcaggaag ccccagaacc tcctgggcaa cctcgacaac accctgcatc acagaagcgc
     1801 cgcctgtcac gactacaccc gctggcagca gatccctgta tctcgcgcga tccagttcat
     1861 ctccgacaag ctccatagtt tcggtaattc tcgcctcaat aatctgacaa agcttcttcc
     1921 tggatatgtt cgtgtccgct ccgcatgatt cgttttcgcc aaccatgtcg ggaagggccg
     1981 acccgtagtt cagcttcaat ccctctgaat agcccacggg caatctcata accaacgcaa
     2041 tgtcgttagt gacgtggtta ccccccacag gaatagatgc aacatgatgc acgccaccct
     2101 caatgcagac tgcgatatcg gtggtcgccc ctcccatatc gatcaccagg gcgccctgtt
     2161 ccttctcaga caacgtcaga accgcctcgg cagcggctat cggttgaagt atgacatgtt
     2221 caacttccag tccaatccgc ctcgcgctcc tgtgaatgtt ctgtatcgca gtagccgacc
     2281 cagtgataat gctggcgtcg acctccaacc ttattcccgt catccccaca ggcttgcgta
     2341 ttcccgagca tccgtctatc acgaattccc ttggcaggac gtgcaccact tctctgtctg
     2401 gcggaatcgc aacgacacgg gcagcctcca acacgctatc gacatcctcc tgggttacct
     2461 cccgatcaga cctaccgata gctactactc ctctgctcgc aatagaagag acatgcgtgc
     2521 ctgacgcgct cgccagcact gaaacagccg cgcaccctga catttcttca gcatgtgcca
     2581 cggcatcagc aatggcggca gccgccgaat ccatgtcaac tatgactcca cggcgcagtc
     2641 ctgcagacgg accactgcct actccgataa tgttcaagtt gtagtcttca tctctttcac
     2701 agacgaccac gtgtatctgg ttcgagccaa tgtcaagccc tactttgata tcgtttttgc
     2761 ccaatacagt tccccccgcc gactccacct gaccctctcg ctttgttgca tgattctaga
     2821 atcgtaccca cttaccctct atcatagcgg gagatttgga agaaccacaa cccccctcag
     2881 tcgatctcct gacgttagca aagaagacgc cagtcccaaa cgcatactcc gatcgcttat
     2941 ctcgatcgga tcaccggttt cgcttcaact cgcaaatcta tgtaatctgc cgacatttcc
     3001 tgttctctaa ctgaagccaa gatcgaatcg agaacccgca cctgtgattc catgtcccgg
     3061 gcgccgccaa tgaatacctt gattccgtct gatgttctga ttattatgtc ttcacacgaa
     3121 gagacgtcta cctcggatat gttgggaatc ccctgtttga gcagggcggc gcctactctg
     3181 gcacccacag acacctcgta tttggggttc accggctgcc ctacctcaac ccaagtcgga
     3241 gcaactccaa cgatgagagg cacatcggga tcggtgatcg cctcagatat gcctattgca
     3301 tacccttctt catcgacgtc tataaaataa cctccgtatg ggagataccc ggcagtgctg
     3361 cgttcctcta ttgcaatgct gatagtatca ggaaggatcc tgtacacttt tgccgtacgt
     3421 atcctaggcg tggccaagag ttttctttcg atctcacctg tcggcaccaa aaacatgttg
     3481 gtattctctg gaatacctgc aatgagaagg acctcgtaat ccgacagata gtttgatccc
     3541 gacactctaa ctgccctaac cagaaaatac ggggagtt
//
LOCUS       DAOSAI010000268         3557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16729_length_3557_cov_4.403484, whole genome shotgun sequence.
ACCESSION   DAOSAI010000268 DAOSAI010000000
VERSION     DAOSAI010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16729_length_3557_cov_4.403484"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1529)
                     /locus_tag="PLM83_08630"
                     /pseudo
     CDS             complement(1..1529)
                     /locus_tag="PLM83_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821898.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1608..1937)
                     /gene="tnpB"
                     /locus_tag="PLM83_08635"
     CDS             complement(1608..1937)
                     /gene="tnpB"
                     /locus_tag="PLM83_08635"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HOK71504.1"
                     /translation="MLSLACGSTDLRKSIDGLAALVQEGFNLDPFAPCLFAFCNRKRD
                     KIKILHWDHNGFRLYYRRLERGRFRWPEKGAETVVVTQRELRWLLDGLSLEQRQAYPA
                     VTARTVL"
     gene            complement(2059..3438)
                     /locus_tag="PLM83_08640"
     CDS             complement(2059..3438)
                     /locus_tag="PLM83_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOK71505.1"
                     /translation="MRHQSAYGSESQPGQVGFTYDANGHLASSTSINGVSTAYATDAQ
                     GRLKSITATRQQPFENILQLGYSYTSQGNVSRISGINGGSPFELNYGYDRINRLTFAQ
                     VPRPGGMANATYQYDGAGNRTVESWSDTGTVQYQYLPGNYLRTRGTTVYSWGPYGQLT
                     SKTQGQTRTDYAYSAQRLMNEVKVDGAALAKYEYDAMGRRIKTIQGETTTITLHSGND
                     IVYEVCTMSQAAGSNAGPNAIPGDPIIVDPIGPIIEDPPTSPVTEYDRLVTCYLVLNG
                     KHLAKVVQQDSDPAEVYFCHTDMLGSIRAITDSSGEVAARFEYEPFGLVIEESGIAAS
                     GAERYTGKPYDQGIGFYFFGARYYDREVGRFTSRDLAKDGLNWYAYCRQNPLVYVDPD
                     GWKIIYADGSTEREKDAFGEAIEYLKEGSGTAQILVNTLEKSEEVIVIWFLKYPLLDP
                     SRKRHSGCR"
BASE COUNT          748 a         1012 c          916 g          881 t
ORIGIN      
        1 gagtatggca gcagctcatc gagtgcagcc gggccgttga tgttaatgtt cggcattctc
       61 tcaaagacgt agcaaaggta agcaaacggg ttcaatccat tctctttggc cgtctgcacg
      121 atgctgtata tgactgcgct ggagtgggct ccacgcggcg tgttcgagaa cagccagttg
      181 cgcctcccga tcacgaacgg tctgatggcg cgttcacagc gattgttgct gatctccaac
      241 cggccatctt gcaggaacgt cgttaacttg tcccactgat tcaggcaata ggaaatagcc
      301 tgacccaagg cgctcttggg caacactttt gttttttgct catgaagcca ctccgagaac
      361 atctctagca ccggtcggct tcgctccagg cgggcctgac ggcgttcctc cggagtcgcc
      421 tcctctaggt cgcgctcgat tgcaaagagg cggttgcaga attccagccc ctgccttgcg
      481 gctactggcg aagcgcgctt gttggctggc aaagcgttta gggcgtcatc gaatttgcgc
      541 cgtgcatgag cccagcatcc tgctagaatc acatcgggcg ttccattgta gccggagtag
      601 ccgtccacat ggagataacc ctcgaacccg gataagaatt cccgagggtg cgagctcgct
      661 cgcgtttgct ggtagtcgta aaggattatg ggatccgcgt atctccctgt gcagtaaacc
      721 catacgtaag attgagtctc tgcagcccga cccggctcat gcagtacctg cagcgtggtt
      781 tcatcagcat gaaggatatc ctgcactaga agatgctcat gcattctttc ataaaggggt
      841 gtgagccatt tcctggcccc gtagatcatc cagttggcca gcgtttgtcg ggaaaggtct
      901 attcccaaac gggcaaactg ctgctcctgg cgatagagcg gcatgccttc aacgtatttt
      961 tgggtcatga tgtaggccag ggctgaaggt gaagccaagc tccttttcaa cacaggcgca
     1021 ggcattggcg ccgttactac aggcgtattg atctctgctt gctcacaccg gcggcaagaa
     1081 tagacgtggc gcacatgctc tacaactttg acctgggcgg ggatgatctt gagctcttgc
     1141 cgcacttcag tgctcatctc atgcaaaggg ccgccacaag agggacagac ctgctcctct
     1201 tctggaaggc agtattccac cgtctccacg ggcaggtcag aaaggatagc ctcgcgccga
     1261 cccttttgtt tgtggcgctg ataggtgatt ttctcgatcg taggttcagc cagaagcggt
     1321 tgatcttccg cctcagcttc gttgaagagc tgcaattatt cgcaatccgt cttttcgctg
     1381 gaagcgccga agcgacgctt ctggctcaag cggaactgct cttcgtacca tttgactttg
     1441 gcagttagct cggcaattat cagcttttgc tgctcgacca gctctttcag ctctgctgtt
     1501 tcgttagtta gcgcgtctat ctcgttcatg atttatgtat tcgacacaaa cctcaacttt
     1561 cctgcaatgt aacgtcatcc cttaaaagaa atattgtgcc ataatattca taatactgta
     1621 cgagctgtca cggctggata agcctgcctt tgctccaatg acaagccgtc aagcagccag
     1681 cggagttccc gttgagtgac aaccaccgtt tccgcgccct tctcgggcca cctgaacctg
     1741 ccccgctcca accgccggta atacaacctg aagccgttgt gatcccaatg cagtattttg
     1801 atcttgtcgc gcttacggtt gcagaaggca aacaggcacg gagcgaaggg gtctaaatta
     1861 aagccttcct ggacaagcgc agctaatcca tcgatcgatt tgcgcaggtc ggtactgccg
     1921 catgccaggc tcaacatgtc gccaatgcct gcactgcctt ggccaacaac tccggatcaa
     1981 agccgggcgt tacctcaatg agtgctttcc caatcttgat ttcaataaca ctggtcggaa
     2041 cttctggtcg gcccatctct acctgcagcc actgtgcctc ttcctcgacg ggtcgagaag
     2101 tggatacttc agaaaccata tgacgatgac ctcttccgac ttctcgagcg tattcactag
     2161 aatttgtgct gttccggagc cttctttgag atactcgatg gcctcgccaa acgcatcttt
     2221 ttcccgttca gtactaccgt cagcatatat gatcttccat ccatccgggt ccacgtatac
     2281 caacggattc tgacggcagt atgcatacca gtttagccca tccttggcta aatcccggct
     2341 tgtgaatctc cccacctcgc ggtcataata cctcgccccg aagaagtaaa agccaatacc
     2401 ttgatcatag ggctttccgg tgtatctttc cgctccggag gctgcaatgc ctgactcttc
     2461 gataaccaac ccgaacggct catactcgaa cctcgctgcg acctcgcccg aagagtcggt
     2521 gatcgccctt atggatccta gcatgtcggt gtggcaaaag tagacttcag caggatccga
     2581 gtcttgctgt actaccttag ccaagtgttt cccgttcagg acaaggtagc atgtgacgag
     2641 tctgtcatac tccgtcaccg gggaagtggg cggatcctca attatcggac caatcggatc
     2701 gactattatc gggtcgcccg ggattgcgtt tggcccagcg ttcgaacccg cagcctgcga
     2761 cattgtgcaa acctcgtaca ctatatcgtt cccagagtgc aaggttatgg tagtcgtttc
     2821 gccctggatc gtctttatcc tgcggcccat ggcgtcgtac tcgtatttcg ccaaagccgc
     2881 gccgtccacc ttcacctcgt tcatcaggcg ctgagcgctg tacgcataat ccgttcttgt
     2941 ctggccttgc gtctttgatg tcaactgccc gtagggcccc caggagtaga ctgtcgtccc
     3001 ccgggtcctc aggtagtttc caggcaagta ctggtactgc actgtgccag tgtccgacca
     3061 gctttcaacc gttcgattgc ctgccccgtc atactggtag gttgcgttgg ccattccccc
     3121 gggcctggga acctgggcaa acgtgagcct gttgatccgg tcgtacccgt agttcagctc
     3181 aaacggagag cccccattga tcccagatat gcgggataca ttcccttggg acgtgtacga
     3241 gtagccaagt tgcagtatgt tttcaaacgg ctgttgcctt gttgcagtta tggacttcag
     3301 cctgccctgg gcatcggtcg cataagcggt gctcaccccg ttgatcgaag tagagcttgc
     3361 aagatggccg ttggcatcgt atgtaaaccc cacctgcccg ggctgggact cagacccgta
     3421 agcagattga tgtcgcatga acacaaacgg tcatgcagca tagtgaacaa aggcatgcaa
     3481 gcaggatggc tgtaatgcgc cgacgggtgg acggcgctct cacgggctca aaatggctag
     3541 tcgcgaagtt gccgaag
//
LOCUS       DAOSAI010000269         3552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16766_length_3552_cov_4.408350, whole genome shotgun sequence.
ACCESSION   DAOSAI010000269 DAOSAI010000000
VERSION     DAOSAI010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16766_length_3552_cov_4.408350"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLM83_08645"
     CDS             complement(<1..625)
                     /locus_tag="PLM83_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOK71506.1"
                     /translation="MGDNDRLRELKAQKEKVIQGGGPKRIEKQHASGKLTARERIERL
                     LDPGSFTELDMFVEHRCQEFGMPEQELPGEGVVTGYGTIDGRKVFIFAQDFTVMGGSL
                     GEMHAKKICKVMDLAMKVGAPVIGINDSGGARIQEGVDALSGYGQIFYRNTIASGVVP
                     QISVIMGPCAGGAVYSPALTDFIFMVQGANMFITGPQVIKTVTGEEVS"
     gene            934..1992
                     /gene="ltaE"
                     /locus_tag="PLM83_08650"
     CDS             934..1992
                     /gene="ltaE"
                     /locus_tag="PLM83_08650"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOK71507.1"
                     /translation="MEWIELRSDTLTLPTEEMRDAMRNAKVGDDVWGEDPTVIRLEEL
                     AAKKVGKEAALFLPSGTMGNLVAVMTHCSRGDEIILEAESHIYYYEVGGLSAVAGAVP
                     RLVLGNSGVLDPDDVRAAIRESNIHYAPTTCLAIENTHNRGGGTVTDLTILAALHDVA
                     KDRGLSIHMDGARIFNAAIALGVDASQIACHVDSVMFCLSKGLSAPVGSMLAGDADFI
                     ARARKNRKMVGGGMRQAGVLAAAGIVALEKMVDRLAEDHANARALAEALVDVPGIEID
                     LSTVETNMVVFDLSRLGIGSNEFAAELSAEGVRVSTRPPHGLRAVTNRHFTADDVERA
                     AQAVRTVCCRHAAPCSGA"
     gene            2103..2609
                     /locus_tag="PLM83_08655"
     CDS             2103..2609
                     /locus_tag="PLM83_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71508.1"
                     /translation="MIGLVSIIIFWAAIVPLITLIHNLGHAAAALALVPEHGVVVRLG
                     SDPRISLVRRGRLEVLLQPWSGWVGRWGWTAGVVEARRAHFVWIMLAGPLASILAALL
                     FGVVKSMFAAGPLFMRDLAQAGMVAAIIQFAASILPVTYPSWLIGYSDMWSDGLRLWR
                     CLAGKTTE"
     gene            complement(3101..>3552)
                     /locus_tag="PLM83_08660"
     CDS             complement(3101..>3552)
                     /locus_tag="PLM83_08660"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOK71509.1"
                     /translation="ETEAYIEQQLRKEHVPSEGQFVIALADDPQLEYVGAVGLHQIDW
                     RNRNCELGIVIGREEMLGQGLGGEAIDLALGFAFDFLNMHKVNLTVFEYNERAHRCYL
                     KCGFRDEGRVRQQRYYDGHYWDMILMGITKDEYVQARRENTTNVCYI"
BASE COUNT          705 a          894 c         1074 g          879 t
ORIGIN      
        1 cgctaacctc ttctcccgtc actgtcttga ttacctgtgg accggtgata aacatgtttg
       61 cgccttgaac catgaatatg aagtctgtca gagcggggga gtaaactgca ccgccggcgc
      121 acggacccat gatcacggat atctgaggta caacgcctga ggctatggtg tttctgtaga
      181 agatctgccc ataccctgac agggcgtcga cgccctcctg gatcctcgct cctcccgaat
      241 cgttgatgcc gatgaccggt gctccgactt tcattgccag atccatgact ttgcagattt
      301 tctttgcgtg catctccccg agggatccgc ccattaccgt gaagtcttga gcgaaaatga
      361 agactttgcg accgtctata gttccatagc cggttacaac cccttcgccc ggcagttctt
      421 gctcaggcat tccaaattcc tggcacctgt gctccacgaa catgtccagt tcagtgaaac
      481 tgcccggatc cagaagccgc tcaattcgct cccttgcggt caatttgccg ctagcatgct
      541 gcttctcgat cctctttgga ccgcctccct gaatgacttt ctctttttgg gccttcaact
      601 cgcggagcct gtcgttatcg cccacataaa ctcacctcgc atcattgttc gaacaggccg
      661 ttgtatctcg gcgtgataca gctgatacat gagaagccac cgcgcatgtg cgcggcgaca
      721 tatggatacc gcttacatta tacacttaga tggcgtttgg ggctaggagc tgggtgggtt
      781 ggatggaaaa tccgatgtaa tattggcacc ctctaaaaca taaaagatgg gaattgactt
      841 tcgagtgtaa tagaggaatg ggacggcgta tgaagtatca ttccaggtaa atgaagagct
      901 ttgtaagacg ctatatgaag ggtggcatgg aagatggaat ggattgagct taggagcgac
      961 actcttacgc tcccaactga agagatgaga gacgctatgc gcaatgccaa ggtcggagat
     1021 gatgtttggg gcgaggatcc caccgtaatc cgccttgaag agctggctgc gaagaaggtg
     1081 ggaaaggagg cggccctgtt cttgccgtct gggacgatgg gtaatctcgt ggctgtcatg
     1141 acacattgca gcagggggga tgagatcatt ctcgaggcgg aaagccacat ctactactat
     1201 gaagtggggg gcttgtcggc tgtggccgga gcggtgcctc ggcttgtctt gggtaatagc
     1261 ggtgtgctcg atccagacga tgtaagggct gcgatccgcg aatcgaatat ccattacgct
     1321 ccaacaacgt gccttgccat tgagaataca cacaatcgcg gaggagggac tgtgactgat
     1381 ctaaccatcc ttgcagctct ccacgatgtt gcgaaggatc gcgggctttc gatacatatg
     1441 gacggtgcca ggattttcaa tgccgctatc gcgcttgggg tagatgcatc ccagatcgcc
     1501 tgtcacgtgg attcggtcat gttctgcctt tccaaaggct tgtcggcgcc tgtaggctcc
     1561 atgcttgcgg gggacgctga cttcatcgcc cgagcgagga agaaccggaa gatggttggc
     1621 ggagggatga ggcaagccgg tgtgcttgcg gcagccggga tagtggcgct agagaagatg
     1681 gtggacaggc ttgccgagga tcatgcaaat gcccgcgcgt tggcggaagc tttggtggac
     1741 gtgccgggga ttgaaatcga tctttcgact gtagagacta atatggtggt cttcgatctt
     1801 tcgcgcttgg ggatcggatc taacgagttc gctgccgaac tgagcgcgga gggggtccgc
     1861 gtcagcacca ggccgcccca tggtctgagg gccgtgacca acaggcattt cacagctgac
     1921 gatgttgagc gggctgctca ggcagtaagg actgtctgct gcaggcatgc cgctccgtgc
     1981 agcggcgcat agcccgtttg agtctgcttt cgggcagtcc gcagcgaagg atgggtcatg
     2041 atatgcaaga cgcccggcga atagaatgtg gtgatcattc tggcctgggg agggacatcg
     2101 agatgatcgg gcttgtatcc atcatcattt tctgggctgc tatagtgccg ctgatcaccc
     2161 tcatccataa tcttggccat gctgcagccg ccttggctct cgtccctgaa catggtgttg
     2221 tggtccgttt ggggtcggat ccccggattt cgctggtcag gcgggggagg ctggaggtgt
     2281 tgcttcagcc ttggagcgga tgggtcgggc gctggggatg gactgcaggt gttgtggagg
     2341 ctaggcgggc gcactttgtt tggatcatgt tggccggtcc tttggcatcg atccttgcgg
     2401 cattgctttt tggcgtagtg aagagcatgt tcgctgcggg gcccctgttc atgcgcgacc
     2461 tggcgcaggc cgggatggtt gcggcgataa tacagttcgc tgcttcgata ttgcccgtga
     2521 cgtatccgtc ttggctcata gggtattctg acatgtggag tgacggcctt cgcctatggc
     2581 gttgccttgc agggaagaca actgaatagc cgcggcgcct ccagcctggc aggagatgga
     2641 ggcggctgag gggaacgagc ggcggtgacg atcctgattt ggaggaagag atcgcaaatg
     2701 gccgggtcgg tgtgaatcgt tcttgttgcg gttgctgcgc acttttcacg ctcatccatg
     2761 aagttgctct tgcgttcgtg ttgcaacatg atgcttccac atggttttag tgcggctgct
     2821 aggtgcatgg gaaggacgaa tcagcctccg attgactcct gcgccggcac ggatcaggca
     2881 gcgccgaagg cctttatggg acctcaggtt tgaacttcag atagtgcagc gcagccccat
     2941 cacattggca ctgctcaaat gctggttgag agttgctctt gttgcgtggg agccgaaatg
     3001 ggcctgtggc tattgcttct ataaaaggac atcattttct ctgcataagg tcattaactg
     3061 aggccgttag ctgaagaagc tgtccgtctt atgctacttc ttagatatag cagacgtttg
     3121 tggtattctc gcggcgtgcc tgaacatact cgtctttcgt gatgcccatg agaatcatgt
     3181 cccagtagtg cccgtcgtag tagcgttgct ggcgtacgcg gccttcgtcc cggaacccgc
     3241 acttgaggta gcaccgatgg gcacgttcgt tgtattcgaa tacagtgagg ttgactttgt
     3301 gcatgttgag gaagtcgaag gcgaaaccga gggcaagatc gatggcttca ccgcctaagc
     3361 cctgtcccaa catttcttcc cggcctatta caattccaag ctcgcagttg cggtttcgcc
     3421 agtctatttg gtgcaagccc actgcgccta cgtactccag ctgggggtca tcggcgaggg
     3481 ctatcacaaa ctggccctcg ctcggcacgt gctccttgcg gagctgctgc tcgatgtagg
     3541 cttcggtctc tt
//
LOCUS       DAOSAI010000270         3542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16834_length_3542_cov_3.485804, whole genome shotgun sequence.
ACCESSION   DAOSAI010000270 DAOSAI010000000
VERSION     DAOSAI010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16834_length_3542_cov_3.485804"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLM83_08665"
     CDS             complement(<1..434)
                     /locus_tag="PLM83_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOK71510.1"
                     /translation="MNRIYLDNAATTPVRREVFEAMVPYLTDKYGNPSSIHSYGREAR
                     ADLNRAREQVAAAIGAAPEEIIFTAGGSEADNLAIKGTALAMYGEKSHIITSAVEHHA
                     VLHSVESLKRLGFSVTILPFDSEGMVDPEDVKRAITPETCLI"
     gene            complement(427..897)
                     /locus_tag="PLM83_08670"
     CDS             complement(427..897)
                     /locus_tag="PLM83_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOK71511.1"
                     /translation="MKLSSRTEYGVRAMVELAEVHGSGPVALREVAEKQGISEKYLEQ
                     LFAALRKEGLIYSQRGAQGGYTLAKAPKHITVGDIVRALEGPIALCDCLADEDGEAIC
                     DRMGECAAHPLWVRLQDGITSILDSTTLQDMIAGDLRDSAKDIGGNKEEERPDE"
     gene            1717..3513
                     /locus_tag="PLM83_08675"
     CDS             1717..3513
                     /locus_tag="PLM83_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71512.1"
                     /translation="MKRSKRLVIQRLALSAILLIFAATGPAHWGCPSFCLAEASSATA
                     NDDATIVGLADLRKCDEQGVPLLIDQVVTVEGAALIDTGKWHNAANYFAIVGPAQPVS
                     LSDWAKAVTPSDRVFGTLVYLPGTTVPQVKAGDFVRVTGKVSIKGYTTDYGTTVIVPS
                     SSEQIVILEHGSDIIGNLPATRLATDPTFAEAEPLEGQLVTVEGRLFGYDDEGITQGF
                     WLDGSRDGNIEDAEGMMQVKFYNYHGLDISHLGNGSYAKVTGVLVQGQDFPPYHGGYY
                     IRPTAQEFITDDPSRKLVSAESEMRAAKIRYNVGDPVHMSKKGIFQLSTMVSSTSYPF
                     VDRVHSHWNPDGSGVVFTMGKLKSARSTLENTETNLFLIDFRGGNEHSQLTFDKSPKE
                     FPRWSPDGTRIAYSALESPDELFGKWDIWIWNASDNSDPIKVTDGLCYDTYPSWSPDG
                     KSLVFQSDRSGNWDIWRIDLDGSVNDDSAIRLTRSMSDDGCPDWSPDGSRIAFQSDRN
                     GNRFDIWIMSLENPENHESPEKNAFCLTGSLSGDSVCPRWSNDGTQIAFMSNHFGTWD
                     IWMADVATGDLWRVTDLEGAEKYPRSPTTDDA"
BASE COUNT          840 a         1023 c          878 g          801 t
ORIGIN      
        1 ctgataaggc aggtttcagg ggttatcgcg cgcttcacgt cttcagggtc gaccatgccc
       61 tcggagtcaa acggaagtat ggtcactgaa aaacccagtc tcttgaggct ttcgacagaa
      121 tgcagcactg catgatgctc cacagccgag gttataatgt gggacttctc gccgtacatg
      181 gcgagagccg tgccctttat ggcgaggtta tcagcctcag atccgccagc cgtgaagatg
      241 atttcctccg gagcggcgcc gatcgctgcc gccacctgct ccctggcacg gttcagatca
      301 gccctggcct cgcgcccgta tgaatgtata ctcgaagggt ttccatactt gtcggtaagg
      361 taggggacca tggcctcgaa aacctcgcgc cttaccgggg tcgtggcggc attgtcaagg
      421 taaatcctat tcatcaggcc tttcctcctc tttgttgccc ccaatatctt tcgccgaatc
      481 cctcaggtct ccggcgatca tgtcctgcag cgtagttgaa tccagaatcg acgttattcc
      541 atcctgaagc ctcacccata gcggatgcgc tgcgcattct cccatcctgt cgcaaatagc
      601 ctcgccatct tcgtccgcga ggcaatcgca cagagctatg ggaccctcga gagctcggac
      661 tatatcgcct actgttatgt gcttcggtgc ctttgccaga gtgtatccgc cttgggctcc
      721 tcgttgactg tagatcaagc cttccttgcg aagagccgca aaaagctgtt caagatattt
      781 ctctgatatg ccctgtttct cggctacctc tctcagcgcc acgggaccgc tcccgtggac
      841 ctcggcaagt tccaccatgg cccgaactcc gtattcagtg cgcgaactaa gcttcatcga
      901 atgtcacgct acggaggccc ccgctctcag gtgcgggggg aagtagcgtt ttcccctcct
      961 ttcgcacagg attggccgat gccgagatcc atgtgccatc caacttgttg cgcaggtcaa
     1021 aacagcctgt gttacgtctt ccgaaaacaa agccctcctc atactcagcc ttatcaaatg
     1081 aaggcgccta tatatttagt caatcaatcc aagtcaatcc ccgcttttgg gcttggggtg
     1141 gtcgaccgta tcacctcccg tcaccgcctg acatagcctg cagattcgac cgccaccgaa
     1201 tcttgcgtgc tgttcacatg tgaacactgc ttctgcctgg ttcggcgtaa tccctactga
     1261 atcagtaggg atttgctact gacagtatac gcccgcatag tatatgtgtc aatagccaac
     1321 ggccttgttt ttctgaactt gcccacaaca tcaacccaag acctcaccaa aaacaaacat
     1381 tgagcgtctt cctcattttg acccattgtc caccaggaac ggggctgact cgaatcacat
     1441 caacttgctt agtgcaacgc tcgaagtaac tatagagtcg acacatcgtc ccattgctga
     1501 ccaagcctgc acgctcagca gctttgcaag acaccatacc ctgtttggac attgaccgga
     1561 atggctgtcc ttgatatgct caacttggca gctatgccat acgcttatat cttcgagagt
     1621 cgcacaaacg ccaagctgtc ggctcagcca ggcgtctgta cgcaggcatg catcgccatg
     1681 cacacgccaa cacaagcaag acgggaggaa tgtggcatga aacgttctaa acgactagtc
     1741 attcaaaggc tggcactttc ggcaatattg ctcatctttg ccgctacagg accagcccat
     1801 tggggctgtc caagcttttg cctggcagaa gcgtcgtcgg ccactgccaa cgacgacgcc
     1861 acaatcgtcg gcttggccga tcttcgcaaa tgcgatgaac agggggtacc ccttctcatc
     1921 gaccaagtag tcacggttga aggagcagcg cttatcgata caggcaagtg gcacaacgcc
     1981 gcgaactact tcgcgatcgt agggccagct caacccgtat cgctctcaga ttgggccaaa
     2041 gctgtcacac catctgatcg cgttttcggc acgctcgtgt atctccccgg aaccaccgtt
     2101 ccccaggtaa aagcgggcga ttttgttcgt gttacaggaa aggtgtctat caaaggatac
     2161 accacagatt atggcaccac ggtaattgtg ccctccagtt ccgagcagat agtcatactc
     2221 gaacacggct ccgacatcat agggaatctg ccggcaactc ggctcgccac cgatccaacc
     2281 ttcgctgaag ccgaacccct cgaaggccag ctcgttaccg tcgaaggaag gctcttcggc
     2341 tatgatgacg agggaatcac ccaaggcttt tggctcgacg gaagccgcga cggaaacata
     2401 gaggacgccg agggcatgat gcaggtcaaa ttctacaact atcatggtct cgacatatcc
     2461 catctaggaa acggctccta cgcaaaagta acaggcgtgc tcgttcaggg gcaggatttc
     2521 ccgccatacc acggcggcta ctacatccgc cccactgctc aggaattcat caccgacgat
     2581 cccagtagga agctggtctc ggcggaatca gaaatgagag ccgcaaagat caggtacaat
     2641 gttggcgacc ctgtccacat gtcaaaaaaa ggcatctttc aactgagtac catggtctct
     2701 agcacctcat acccattcgt agaccgagtt cattctcact ggaatccaga tggcagcggc
     2761 gtcgtgttca ccatgggaaa actgaagagt gctcggagca ctctggagaa tacagagaca
     2821 aatctatttc tcatcgattt tcggggcggg aatgagcatt cccagctcac cttcgacaaa
     2881 agccccaagg aattcccgcg atggtctccc gacggaacca gaatagccta tagcgcgttg
     2941 gaaagtcccg acgaactgtt tggcaagtgg gacatttgga tatggaacgc gtcagacaat
     3001 tcagatccca taaaagtgac tgatgggcta tgctatgata cgtatccaag ctggtcgcct
     3061 gatggaaaga gcctcgtatt ccagtccgac cgcagcggca actgggacat atggcgaata
     3121 gacctcgacg gcagtgtcaa tgatgatagc gcaatccgtc tcacccgatc aatgtcggat
     3181 gatggctgcc ctgactggtc ccctgatggt tcaaggattg cgttccaatc agacagaaat
     3241 ggcaacagat tcgacatatg gatcatgtct cttgagaatc cggagaatca cgagagtcct
     3301 gagaagaacg ctttctgcct gaccggatcg ctctcgggcg actctgtttg cccacgctgg
     3361 tccaatgacg gcactcaaat agcattcatg tcaaatcact tcggaacatg ggacatatgg
     3421 atggccgacg tagccacagg ggatctgtgg cgcgtgactg atcttgaggg cgctgagaag
     3481 tatcctcgtt ctcccacgac ggacgacgca taatattcgc cactgatctg ggacaaggct
     3541 gg
//
LOCUS       DAOSAI010000271         3535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16877_length_3535_cov_3.968678, whole genome shotgun sequence.
ACCESSION   DAOSAI010000271 DAOSAI010000000
VERSION     DAOSAI010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16877_length_3535_cov_3.968678"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLM83_08680"
     CDS             <1..218
                     /locus_tag="PLM83_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HOK71513.1"
                     /translation="GLLDAARETLTGALRRRKARSEELLRALRYERALVYEDLGQRRR
                     ARSELEKLYAEDPDYEDVAMRLGIDQT"
     gene            230..1903
                     /locus_tag="PLM83_08685"
     CDS             230..1903
                     /locus_tag="PLM83_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK71514.1"
                     /translation="MRIARVHIEHFRSIQELDFDPGPYCVLIGENNSGKSNILRALNL
                     VLGETWPTEWSFSEEDFHNQDTSKDIVIRVVFDGVTEEWRNKHKMVVGGFELRCHALE
                     RKSGKKQRGDLSVSYVCVDEKGKVCQFPKEPGKYKGQWVPLRVSSALREQAASMIYVG
                     VLREYDKQTPSSRWSVLRRLFNEVNTEFLNDKKKITVKLPDGTSKKMTRKEAFEAAVR
                     DAYKYLRTESFEEIERRLAENGIEQMGLDADKNKVELHFESHDPTNAFKSLQLYVDQM
                     GIRSPAGEVGAGLQSAIVVAIFRTYEELKKEGAVFAIEEPEAFLHPQKARYFASVLRS
                     LAEKGNQVFLTTHSPVFVQIHDPESVAIVRRTVEIGTKVHQASKVELAEDERKSLRLM
                     TEFDSQRNELFFARRVMFVEGNTEKVALPLVFRALGIDINKENISVIECGGKTKIPLF
                     VRVARALGIPYIVLADCDIQEIKEEWSPKRKETQREKNRKHEQWNKAILDAAGKEHTF
                     WLAPTFEGALGLPKDESQKVDQALKRFANATEEDIPDCLKEPIRRLMEI"
     gene            2320..2895
                     /locus_tag="PLM83_08690"
     CDS             2320..2895
                     /locus_tag="PLM83_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71515.1"
                     /translation="MVADQFEELLKRMSKIAEVVNGFKSEAVQAEVYRTLVGVLGAGD
                     LDDNSDDDGKAANRTRHARPKRKANDSSSESVDTESASVDTNDIVNTMKERADFSELS
                     SKVLHARDLWDKIRLILYFADSAMTSGEIYRVLQGFDIKTTLPAVSKKLKEMHGQLIS
                     SGTRRPGAVIRYKLSGPAKSEAEKWMNEIVK"
     gene            2879..>3535
                     /locus_tag="PLM83_08695"
     CDS             2879..>3535
                     /locus_tag="PLM83_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71516.1"
                     /translation="MKSSSEFHQFAAYLLGQKKLREVDKAVACIWFAGYHGHAEEMSA
                     KAVADCMAAVGLSGNVNISRLWKALTKSKFTVKGTNSNAFRISPVKKHLLDEQYEPLL
                     KQRKVKVSDSLLPDAVIQDTRKYLQDLARQINGTYDAAYYDACAVLCRRLTESLLIEG
                     FQAAGQLTAIQRPDGTLEMLDSILAKAKSGQFIRLPRGTPDTLDKIKRIGDTAAHDRF
                     H"
BASE COUNT          895 a          852 c         1033 g          755 t
ORIGIN      
        1 tcgggctcct ggacgcggcg cgggagaccc tgacgggcgc cctgcgccgg aggaaagccc
       61 gctccgagga gctgctccgg gcgctccggt acgaacgggc cctggtctat gaggacctcg
      121 gccagcgccg gcgggcgcgc agcgagctcg agaagctcta cgccgaggac ccggattacg
      181 aggacgtggc gatgcggctc ggaattgatc aaacataagg gttgatgtaa tgcgtattgc
      241 ccgggtacat atcgagcatt tccgttcaat ccaggagtta gacttcgacc cgggaccgta
      301 ttgcgtctta ataggtgaga acaattccgg gaaatcgaac atcctccgag cactgaatct
      361 tgtgcttgga gagacttggc ctaccgaatg gagcttctca gaagaagact tccacaacca
      421 agatacaagc aaggatattg taattcgcgt cgttttcgat ggagttactg aggaatggcg
      481 aaataagcac aagatggttg tgggcggttt tgaactaagg tgtcatgctt tagagaggaa
      541 atccggcaag aagcagcgtg gcgatctaag tgtttcctac gtatgcgtgg atgaaaaagg
      601 caaggtctgt caattcccga aggagccagg aaaatacaag ggacagtggg tgccgttaag
      661 ggtctcctcg gctctgcgtg agcaggctgc atccatgatt tacgttggcg tgttgcggga
      721 atatgacaaa cagaccccca gcagccgatg gtcggttctc cgccggctct tcaatgaggt
      781 taacacagag tttctcaacg acaagaagaa gataacggtt aagcttcctg atggaacttc
      841 caagaagatg actcgaaagg aggcattcga ggctgccgta agagatgcct acaagtacct
      901 gagaacggag tctttcgagg aaatcgaacg acgactggcg gagaatggga ttgagcaaat
      961 ggggttggat gcggataaga ataaggttga actgcacttc gaaagccacg accccaccaa
     1021 cgctttcaag tcgctacagc tttatgtcga ccagatgggt attcgtagcc ccgctggaga
     1081 agttggagcg ggtttacaga gcgcgattgt cgtggccatc ttccggacct acgaagaatt
     1141 gaagaaggaa ggtgccgtgt tcgctatcga ggaaccagag gccttccttc atccacaaaa
     1201 agcccgttat ttcgcctctg tgcttcgatc actggcagag aaaggtaatc aggtcttctt
     1261 gaccacgcac tcgcctgtgt ttgtacagat tcacgatcct gagtccgtcg ctatagtccg
     1321 cagaactgtg gagatcggca ccaaggttca ccaagccagc aaggtggagt tggctgaaga
     1381 tgaacggaaa tcgctccgct tgatgacgga atttgactcg cagcgcaacg agctgttttt
     1441 cgcgcgaagg gtgatgtttg ttgagggaaa cacggaaaag gtggcgctcc cccttgtgtt
     1501 tcgggcgctc gggatagaca ttaacaaaga aaacatttcc gttatcgaat gtggcggcaa
     1561 gacaaagatc cccttgtttg tgcgcgtagc gcgtgcgctc ggtattccgt atatcgtgtt
     1621 ggcggattgt gacattcaag agatcaaaga ggagtggtcg cccaagcgta aggaaactca
     1681 gcgagaaaaa aacaggaagc atgagcaatg gaataaggct atccttgatg ccgctggaaa
     1741 ggaacataca ttctggttgg caccgacttt tgaaggggct ctgggattgc ctaaagacga
     1801 gagccagaaa gtcgaccagg cgcttaaacg ttttgccaat gccacggaag aggacatccc
     1861 ggattgcttg aaagaaccca taaggaggtt gatggaaatt tgacaccgtg ccgcggcaga
     1921 agctcgtatg gacattatag gaaattggcc ttgacgcctt ttccgccccc ttgggaaatt
     1981 gccgctcttt gaaaagatgt tgtgtatcaa cgggttaggt tatatcctgg cggtggagag
     2041 tggttatgaa gagggttgca ggtggggagt tcaagccgca agccgttgta aataagcggc
     2101 ttgcggctta tttcgtttcg ggtcgcatcg aaccctcttc aatccgcaag gttcgacacg
     2161 accaaacgtt gtgacccggc cgattcacgg ggcaggccgc agttcgacct ctctcgctga
     2221 tccgccagtt gacggagccg agggtctatc ggatccctat tgggagaaac tctcagactc
     2281 tcgcgcggtg ttcagacgcg ggttaacagg agaccaccga tggtcgcaga ccagtttgaa
     2341 gaactgctga agcgcatgtc gaagatcgca gaggtcgtga atggattcaa gtcagaggcg
     2401 gtgcaggcag aagtctacag aacgctcgta ggtgtgcttg gggctggtga tctagatgac
     2461 aattcagacg acgatggcaa agccgcaaat cgcacacgtc acgcacgtcc taagcggaaa
     2521 gcaaacgatt cgagcagcga gtctgtcgac acagaatccg cttcggtcga caccaacgac
     2581 atcgtgaaca cgatgaagga gcgtgctgac tttagcgagc tctcttcaaa agtgctgcac
     2641 gcccgggatc tctgggacaa gatccgcctg attctctact ttgcagattc agcgatgacg
     2701 tcgggcgaga tttacagagt gctccaaggg ttcgacatta agaccacctt gcccgcggtg
     2761 tccaagaagc tgaaagagat gcacggacaa ttgatttcat ctggaacacg tcggccgggg
     2821 gccgtgattc gatacaagct gtcaggcccc gcgaaaagtg aggcggagaa gtggatgaat
     2881 gaaatcgtca agtgagttcc atcaatttgc tgcttacctg cttggccaga agaagctcag
     2941 agaagtcgac aaagcagtcg catgcatctg gttcgcaggc taccacggcc acgctgagga
     3001 gatgtcagca aaggccgtgg ccgactgcat ggctgccgtt ggattgtcag gcaatgtgaa
     3061 catttcgcgg ctatggaagg cacttaccaa gtcgaagttc acagtcaaag gcactaactc
     3121 gaatgccttt cgcatctctc ccgtcaagaa gcatctgttg gatgagcagt atgagccgtt
     3181 gctcaagcag cggaaggtga aggtaagtga tagtctgctt cccgacgcag tgattcagga
     3241 cactcgtaag taccttcaag acttggcacg gcagatcaac ggcacctatg acgccgccta
     3301 ttacgatgca tgtgccgtcc tgtgtcgtcg tctgaccgaa tcactactga ttgagggatt
     3361 ccaggcggcc ggacagctta cggcaataca gagaccagat ggaacgcttg agatgctcga
     3421 ttcgatcttg gccaaggcca agagcggtca gttcattcgc cttcctcgcg gcacccccga
     3481 tacgctcgac aaaatcaaga ggatcggtga tacggcagca catgaccgct tccac
//
LOCUS       DAOSAI010000272         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_16901_length_3533_cov_3.649799, whole genome shotgun sequence.
ACCESSION   DAOSAI010000272 DAOSAI010000000
VERSION     DAOSAI010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16901_length_3533_cov_3.649799"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /gene="fliR"
                     /locus_tag="PLM83_08700"
     CDS             complement(<1..768)
                     /gene="fliR"
                     /locus_tag="PLM83_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOK71517.1"
                     /translation="MAEGTLGFTEREFLLFFLVLVRMTGLFILAPLFGHASVPARAKA
                     GLAFACALVVYPMMAQTPFEMPTDVYELTSDVARELFVGATLGFVSLLVLAAAQYGGE
                     MIDTQIGFSLANIVDPSFGQQVSLLGQFHYLVAMMVYMAVDGHHYLIGALMRSYSVIP
                     IGGAGMSSELFSLVLARFSDLFMIAIRMSLPPVACLLITEIALGVLAKSVPQMNVLMV
                     GFPAKIVVGLAVVGLAIPGFVGCAATAFASWRKAIVVL"
     gene            complement(790..1059)
                     /gene="fliQ"
                     /locus_tag="PLM83_08705"
     CDS             complement(790..1059)
                     /gene="fliQ"
                     /locus_tag="PLM83_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOK71518.1"
                     /translation="MNEAMVAALASEAVSVAFKVSLPVLLTALVVGVLVSVLQAATQI
                     QELTLTFVPKLLAIAAVLLLTGSWMTSTLTGFAREVFAMLPSLAK"
     gene            complement(1087..1821)
                     /gene="fliP"
                     /locus_tag="PLM83_08710"
     CDS             complement(1087..1821)
                     /gene="fliP"
                     /locus_tag="PLM83_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722487.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOK71519.1"
                     /translation="MAGIGIALACSAVAYCQPMPIPNIRIGVDAAESPTEVAGALRII
                     ALLTVLSIAPAILLMTTSFTRIVIVLSFVRNAMGTQQVPPNQVLIGLALFVTLFVMMP
                     VYSQINEEALKPYMAGIISQEEAMARGVAPLRSFMLKQTREKDLALFVDLAGLPKPGG
                     PEDIPTYVIMPAFMVSELKTAFQMGFVIFIPFLVIDMLVASVLMSMGMMMLPPVLVSL
                     PFKVLMFVLVDGWGLVVKSLVESVAR"
     gene            complement(1896..2297)
                     /locus_tag="PLM83_08715"
     CDS             complement(1896..2297)
                     /locus_tag="PLM83_08715"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOK71520.1"
                     /translation="MEESMAMLLLRAALALAAVAGLIYLVAWLLKKARASGLIKVSGP
                     ERRIRLIETARLGADSYLHLAEVDGKIALIGVGRGGISIVQWPRGSGQEGTGDMPDGD
                     GGLSAALYGQTDEESTDSQDGGGSEGGVDAV"
     gene            complement(2300..2548)
                     /gene="fliN"
                     /locus_tag="PLM83_08720"
                     /pseudo
     CDS             complement(2300..2548)
                     /gene="fliN"
                     /locus_tag="PLM83_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009965.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
     gene            complement(2778..>3533)
                     /gene="fliM"
                     /locus_tag="PLM83_08725"
     CDS             complement(2778..>3533)
                     /gene="fliM"
                     /locus_tag="PLM83_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOK71521.1"
                     /translation="TPVKVTLIDVQQRIYDEYLSSLKSPAVMYVFTPEPLDGQAIIEF
                     NPYIVFIMIDRLMGGPGRVRGRVRELTEIEEKLVRAMVDRAMDDLKDAWDPMVRFDPH
                     IDAYESNPQFTQIVSPNDTTCVISFEIRVGEANGSMSICMPHSTLQPVLQKLSAQQWY
                     QKGTKISSEKSANAISAGLEGVKVPIAACLGRASITVSEMLELQTGDVIKLDEHAFDP
                     LTVVIGNSPKFHAIPGLVGKNYAIQIVGTVEST"
BASE COUNT          853 a         1103 c          835 g          742 t
ORIGIN      
        1 cagcacgaca atagcctttc gccagctcgc aaacgccgta gcagcgcacc caacaaaccc
       61 cgggatcgcc agcccaacaa ctgcaagacc aaccactatt tttgcgggga atccgaccat
      121 aagcacgttc atctgcggaa cggacttggc cagcaccccc aacgctattt cggttatgag
      181 caagcaagcc actgggggta gcgacatcct gatggcaatc atgaagagat cggaaaacct
      241 tgcaagcacc agcgaaaaaa gctcagacga catcccggcc ccgccaatgg gaatcaccga
      301 atagctacgc atcagggcac cgataagata gtggtggccg tctactgcca tatacaccat
      361 cattgccaca aggtaatgaa actgccccag caagctgact tgctggccga atgaaggatc
      421 tactatgttt gccaaactga agccgatctg agtgtctatc atctcgcccc catactgagc
      481 ggccgcgaga accagaagcg acacgaaccc gagcgtcgcc cctacaaaaa gctctctggc
      541 cacgtctgac gtcaactcat acacatcagt cggcatttcg aaaggcgtct gggccatcat
      601 gggataaaca accaatgcac atgcgaaagc aagccctgcc tttgcccgag cgggcacgct
      661 ggcgtgccca aaaagcggtg ccaggatgaa caagcctgtc atcctcacaa gcaccaggaa
      721 gaaaagcaaa aactcccttt ctgtgaatcc caatgtgcct tcagccacct taagcaccgc
      781 ctgtatagac tactttgcca acgaaggcaa catagcgaag acctcgcgcg caaaccccgt
      841 gagggtgctg gtcatccacg atccagtcag aagcaatact gcagcgatgg ccaacagctt
      901 aggaacgaaa gtgagtgtga gctcctgtat ttgcgttgcg gcctgcagca ctgatacgag
      961 aactcccact accaatgcgg taaggagcac cggcaaactg actttaaatg ccaccgacac
     1021 cgcctccgac gccagtgcag caaccatcgc ctcattcatg ttcacccctc caaactccca
     1081 ccatgcttac ctggccacac tttccaccaa cgacttcaca accaatcccc atccgtccac
     1141 cagcacaaac attaaaacct tgaatggcag cgaaacgagg accggcggga gcatcatcat
     1201 ccccatcgac atcagaacgc tcgcaacgag catgtcaatg acgagaaatg ggatgaatat
     1261 gacgaagccc atttgaaatg ccgtcttgag ttcgctgacc atgaatgccg gcattatcac
     1321 ataggtcggt atgtcctccg gcccgcccgg ctttggcaat ccggcaagat ccacgaagag
     1381 cgcgaggtct ttctcccgcg tctgcttgag cataaaactg cgcaagggtg ctaccccgcg
     1441 ggccatggct tcctcctggc ttatgatgcc ggccatgtaa ggcttcaatg cctcctcatt
     1501 tatctgagaa tagacaggca tcatcacgaa caaggtaaca aagagggcca gcccgatcag
     1561 gacttggttc ggcggcacct gttgcgtgcc catggcattt cggacaaatg agagaacaat
     1621 gactattctg gtaaacgaag tcgtcatcaa gagtattgcc ggcgcaatcg acaataccgt
     1681 tagcagagcg ataatccgca gtgctcccgc tacttctgtc gggctctccg cggcgtccac
     1741 ccctatcctt atgttcggga taggcatggg ctggcagtag gccactgccg aacatgcaag
     1801 cgctatccct attcccgcca taatcgcccc tatccaaaca agcgctgtcc ttgctgagcg
     1861 gggtatccga gtctctaacc gggcgcgtct cttgttcata cagcgtccac cccgccttcg
     1921 cttcctccac catcctggct gtcggtgctc tcctcatcgg tttggccata cagggcagcg
     1981 ctcaagcccc catcgccatc aggcatgtcc cctgttccct cttggccact gccgcgtggc
     2041 cattgaacaa tactgatccc gccacgcccc acgcctatca gcgcaatctt gccatccact
     2101 tcagcaaggt gcagatagga atcggcgccc aggcgagcgg tttcaatgag gcggatacgt
     2161 ctttccgggc ccgatacctt tatcagcccc gacgcacgag ccttcttgag aagccaagcc
     2221 acaagataaa tgaggcccgc aaccgctgcg agggcaagcg cagcgcgcag gagcaacatg
     2281 gccatactct cttccattcc tattgcactg ctttctccgg tgccttgacg aactccacga
     2341 tcctcacccc gaaattctcc tcaatcacca caacctcgcc ccgggcaatc agccgaccgt
     2401 tgatgaggat gtcaacattc tctcctgccg ccttgtcaag ctcgattatg gaccctggcc
     2461 cgacagacat tatgtccttc acaagcatgg tagtccgccc gagctcgacc atgaccggaa
     2521 gcggcacatc catgagcagg tccagattgt cgcctttccc gccttccatg ccgggttcgt
     2581 caaatggcat gaactggacg ggctgtacat ttgcctgttc ctcctgcacc tgcttggatg
     2641 atctcactgc agactgagcc gactggtttg agtgtgacgc ctccggaacc tcaggcgtca
     2701 gatcaaactg aggaacagca tcatcatcag tctgcatgtc gtcccacgag aattcactca
     2761 tgacattccc ctcatttcta cgtagactct acagtgccaa cgatctggat tgcgtaattc
     2821 ttcccgacaa gccccggaat cgcatgaaac ttcgggctgt ttccgatcac aactgtgaga
     2881 gggtcgaagg catgctcatc cagtttaata acatcgcccg tctgcagttc cagcatctcg
     2941 ctgacggtga tcgacgccct tcccaaacag gccgcaatgg gaacctttac tccctcgagc
     3001 ccagcgctga tggcgttggc cgacttttcg gaactgatct tggtgccttt ctgataccac
     3061 tgctgggcac tgagcttctg aagcacaggc tgcaacgtgg aatgcggcat gcaaatgctc
     3121 atggagccat tggcttctcc caccctgatc tcgaatgaga tgacgcacgt ggtatcgttt
     3181 ggcgaaacta tctgcgtaaa ctgtggattg gattcatatg catcgatatg gggatcgaat
     3241 ctcaccatag gatcccaagc gtctttcaga tcgtccatgg cccgatccac catcgcgcgg
     3301 acaagcttct cctctatttc agtgagctcg cgcactcgtc cccgtacacg tccggggccg
     3361 cccatgagcc tgtctatcat tatgaaaact atgtacgggt taaattctat tatggcctgc
     3421 ccgtcaaggg gctcgggtgt aaagacatac atgactgccg gcgacttcag agagcttaag
     3481 tattcatcgt atatacgctg ttgtacatcg atgagcgtca ccttcaccgg cgt
//
LOCUS       DAOSAI010000273         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17019_length_3514_cov_4.079792, whole genome shotgun sequence.
ACCESSION   DAOSAI010000273 DAOSAI010000000
VERSION     DAOSAI010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17019_length_3514_cov_4.079792"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..765
                     /locus_tag="PLM83_08730"
     CDS             247..765
                     /locus_tag="PLM83_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOK71522.1"
                     /translation="MEDALELVAQLMEISARTAPKAVGKDFVETKVVAGDDLKSLAEQ
                     MELYGQETGKKNYDRDARGVAAAGALLLVGLKDAAPCGLNCGACGYSACADLPDLAEG
                     REFAGPICAWRLVDLGIALGSAAKTASIHNVDNRIMYRIGVVARKMGLIDADVACGVP
                     LSASGKNPYFDR"
     gene            complement(1296..2075)
                     /locus_tag="PLM83_08735"
     CDS             complement(1296..2075)
                     /locus_tag="PLM83_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71523.1"
                     /translation="MNLGTVFGAVFRRELTMIRRYMVNSISSLVSVYLVFILMFMGFQ
                     SMSNYVKTGALEGSLEGTIVGFFVWTFTIYAFSDLSWSLISEAQAGTLEQLYLAPCGF
                     KWVGACTVISNFIFSLVPIMVLLFAMMATTGKWLNLDFVSLIPLLLITLLGAYGFGFA
                     LGGLALVFKRIQAFFQIMQFVFVGFLIVPTRLAWAKFLPLAVGNDLISTVMVDGARLW
                     ELPLNDILTATAVGAAYLAIGIVIFSCCENVAKSRGLLGHY"
     gene            complement(2072..3049)
                     /locus_tag="PLM83_08740"
     CDS             complement(2072..3049)
                     /locus_tag="PLM83_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71524.1"
                     /translation="MLDITRNALAVESLTKTFRDGGRGSVKAVDNVAFQVEQGEVVGL
                     LGPNGAGKTTTIKCILGLMRPDSGHVTVFGEDAQTNYPRILSKMSAVLEGSRNLYWRL
                     TVWENILFFSALHGVASPADKRYFDHLLKLFSLEDKRNVEVRKLSQGMKQKTSVVCAL
                     ARRAPLVFLDEPTLGLDVETSLELRSVLRELSCEEGRTFVVSSHDMAVIQDVCQRVII
                     MSEGRIVVDDNVSHLMDLFRTRCYRIVLRGPAPEGLEKRLVHEFVGSSVAEGEQGAEI
                     KVNLSEDEDIYRLMDILRGVDGVIDEISHEEIDMEKVYLEVVRKERSFQ"
     gene            complement(3308..>3514)
                     /locus_tag="PLM83_08745"
     CDS             complement(3308..>3514)
                     /locus_tag="PLM83_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71525.1"
                     /translation="EVHWTTPYHGQSKPIERAWKTLCEEVSKHPKFAGALSDSVEAGV
                     EVWAWRCDVGLDRISLDAEVPVNI"
BASE COUNT          835 a         1040 c          904 g          735 t
ORIGIN      
        1 ttggcgtgaa gcagcgcgcg gtcatcacca tggaagtcct ctcttgggac gccgccgatt
       61 gtccgctctg ccgcggggga tcggagccgg tgaagcctgg aagccgcaag tagctgctaa
      121 tccgtgccat cgtccccttc taagaggaat ttgcctattg gccgagtagt atgtctacag
      181 cataccattt gaaaggaggc agggcgccag cccgggcccg cacggcccgg gagcgcgaag
      241 aggaatatgg aagatgcgct tgagcttgtg gcccaactga tggagatttc ggcgcgaacc
      301 gcgcccaagg cggtcgggaa ggatttcgtg gaaaccaagg tagtggcagg cgatgatctg
      361 aaaagcctgg cagagcagat ggaactctac ggccaagaga ccggcaagaa aaactatgac
      421 agggatgccc gtggcgttgc tgcagccggg gcgctgctcc ttgtaggctt gaaagatgca
      481 gccccctgtg gcctcaattg cggcgcatgt ggctattctg cctgtgcaga cctgcccgat
      541 ctcgctgagg gtcgggagtt tgctggaccg atctgcgcat ggcgcctagt tgacctcggg
      601 attgcgctgg gatcagctgc gaaaacagcg tcaatacata atgtagacaa caggatcatg
      661 taccggatcg gggttgtagc ccgcaagatg ggcctcatag acgcagacgt agcctgcgga
      721 gtgccccttt cggcatccgg aaaaaaccct tacttcgacc gttaggcgca gtctgtccta
      781 aagaccatcg cctgagcctg caaacccacc gatcaagtat tacggcaagg agcgtcgatg
      841 caacaccatt gacgcttctt gccgcttcca cttgccccgt cattcagctg tccacaaggc
      901 acaagacccg ctttcatgcc atgtttgcct cgtgaagatg atcaactttg tcggcctcga
      961 caacatcacg agtgaccaga atgatcgtca agcatttgct tctgcaaaaa ccccatacaa
     1021 tcataggcgc tatttgaggg ttttcattgc ccaagatatg tagagccgct gacggggcgg
     1081 agcacgcccc gttgtccggc caaggccgaa aacgcccagg ctttagcctt tcaacactcg
     1141 caggttgata tgggagttgg ctgccgcccg agatccacga taaatcttgc agagtctctg
     1201 gacagcgcag acatcttcga ttccccgcac gcttgccctc cttgccaata tcaggagaga
     1261 taatgccctc gatccggcaa tcgatcgccg aaagatcaat aatgaccgag aaggccacgg
     1321 ctcttggcga cgttctcaca acacgagaat atcacgattc cgatggcaag atatgccgcg
     1381 cccactgcag tggcggtcag aatgtcgttc aatggaagct cccataggcg ggcgccgtcg
     1441 accatcactg tgcttatcag atcatttccc acagcgagcg gcaggaactt agcccacgcc
     1501 aggcgtgtcg ggactatcag aaagccaacg aatacaaact gcatgatctg gaagaacgcc
     1561 tgtatccttt tgaacaccag ggccaggccg cccagggcaa agccgaaccc gtatgcgccc
     1621 agcaacgtaa tgagcaacag tgggatgagg ctgacaaagt cgaggttgag ccatttgcca
     1681 gtagtggcca tcattgcaaa caggagaacc attatcggaa cgaggctgaa gatgaaattg
     1741 gatatgaccg tgcaggcccc cacccatttg aacccgcatg gcgccagata cagctgttcc
     1801 aaggtgcccg cctgggcctc gcttatgagg ctccacgaca ggtcggagaa ggcatatatc
     1861 gtaaacgtcc acacgaaaaa cccgacgata gtgccttcaa gggagccttc cagtgccccg
     1921 gtcttaacgt aattggacat gctttgaaag cccatgaaca tcaagatgaa gacaaggtat
     1981 accgacacta agcttgatat ggagttgacc atatacctcc tgatcatcgt caactcccgc
     2041 ctgaagacag caccaaaaac agtgcctagg ttcattgaaa cgaccgctcc ttcctgacga
     2101 cctcgagata caccttttcc atgtcgatct cttcgtgaga gatctcgtct atgactccgt
     2161 caacgccgcg caagatgtcc atcaggcggt agatatcctc atcttcagac aagttcacct
     2221 tgatttcggc gccttgctcc ccctcagcca cagacgaacc aacaaactcg tgtacgagcc
     2281 ttttctccag cccctcgggt gcaggtccgc gaagaacgat ccgataacac ctcgtccgga
     2341 agaggtccat gaggtggctc acattatcgt ccacaactat gcggccctcg gacattatga
     2401 ttacgcgctg gcagacatcc tggatcacgg ccatgtcgtg gctgcttact acgaacgttc
     2461 ggccctcttc gcaagaaagt tcccgcaata cgctcctcag ctccaaagat gtctcgacat
     2521 ccaaccccaa agtgggctcg tccagaaaga ccagcggggc gcgacgcgcc agggcgcaca
     2581 caacgctcgt cttctgtttc atgccctgcg acagtttcct cacttctaca ttgcgcttgt
     2641 cttccaatga aaagagcttc agaaggtggt cgaagtatct cttgtctgcc ggggatgcca
     2701 ccccgtggag cgcggagaaa aagagaatgt tttcccacac cgtcagccgc cagtagaggt
     2761 tccggctccc ctcaagaacc gcactcatct tcgagagtat ccgcggataa ttcgtctgtg
     2821 catcctcgcc aaacactgtg acgtgtccgg aatcgggacg catcaacccg aggatgcact
     2881 tgatcgtggt cgtcttgccc gcaccgttcg gcccgagcaa gcccacgact tcgccttgtt
     2941 cgacctgaaa agcgacattg tcgacggcct taaccgaccc acgacccccg tcgcgaaacg
     3001 tcttcgtcaa agactccact gcgagagcat tgcgtgtaat gtccaacacc aagccacctc
     3061 ccccatcttc cggctgccct gcccatgctg cgccttcagg catcttattc ctgggatctg
     3121 cgccaggcag tggataacat gagtttcaat tccaccgttt caatcctaaa tgatgtattc
     3181 aatattgtca agtctatagt ccctaatatt aaacactcag taacatttat tgattacgcc
     3241 tgtattgtgc agcaaaccac atatgttgga tggagggaac ttccatggcg agctgtcaga
     3301 gacgatgtca gatgtttaca ggaacttctg cgtcaagcga gatcctgtcc aaaccgacgt
     3361 cgcaccgcca tgcccaaacc tcaaccccgg cctccaccga atcgctcaag gcgccagcga
     3421 acttcgggtg cttcgagacc tcttcgcaga gcgtcttcca ggcccgctcg atcggcttcg
     3481 actggccgtg atagggcgtc gtccagtgga cctc
//
LOCUS       DAOSAI010000274         3489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17199_length_3489_cov_2.985149, whole genome shotgun sequence.
ACCESSION   DAOSAI010000274 DAOSAI010000000
VERSION     DAOSAI010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17199_length_3489_cov_2.985149"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /gene="recJ"
                     /locus_tag="PLM83_08750"
     CDS             complement(<1..1550)
                     /gene="recJ"
                     /locus_tag="PLM83_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOK71526.1"
                     /translation="MAPRRTIWELREGDSAAEKRLAATCGISPVLARVLANRGIVDEK
                     SVGAFLMPDPSEMHDPFAMPDMEQSAARLWAAVEIGEGIRVYGDYDVDGITSVALLVS
                     VLRSLGANVDYHIPNRLVEGYGLNREAIEQAAGDGVSLLVTVDCGVSAIEEVDYASEL
                     GIDVIITDHHEAGSILPSCVGVVDPKRHDSRYPFKELAGVGVAFKLAQAVWTLAGRPP
                     CAADPHKYLDIVALGTIADVVPLQGENRIIAKAGLELMGRSSNHGINALLDVSDLSGR
                     EIRAGHVSFTVGPRVNAAGRLGDPSLGARLLLTDSAQEAAELAAMLDEENRKRQEIEL
                     SILREAWVPVSKMNTQTTRAIVLGSDRWHSGVIGIVASRIAELTYRPTVLISFEGDVG
                     RGSARSIPAFNLYSALTECSDLLLKYGGHSQAAGLSIKAEDLDLFRERLNEIGHEWLT
                     EEDLVPRVLVDAELGEDEICIELAMELERLEPFGYGNPTPLFVTRNMHVLERRNVGPD
                     GKHLKLKLG"
     gene            complement(1626..3374)
                     /locus_tag="PLM83_08755"
     CDS             complement(1626..3374)
                     /locus_tag="PLM83_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOK71527.1"
                     /translation="MNIDALLSFAREINGELDLGRGVRIGETALVSRCVHVLDPHCPG
                     QAQVQCRLDAGQVFYQRSSTDEVVHIDIFHELADVDHRTAAEAIRRAVEAYGQQMSVL
                     PRPLLELGGPIWSIAGLADFIDVQTATGGGRVTGSLNFGQKESLKIAHRGHVHVAAFL
                     PPEMVGLVYYIVREVEASIVSSGVGLRRLEQIENASSSESSPVDLSPYSCSTDSYMRG
                     QIPYDGGNSLPDLMKQALEVCTDFGSVNSFKSFLECVAGPGRRENPYAMLEREFGDAE
                     GMLAKLRTLEIVEGEGAALVLTAKGRRLALFLKKFAREVENQVRRIIRRSARPRGTPL
                     ERSDFSKGMRAGNGPGIVRTVIPASEVDYRGSISVAETVRSWAVRCATEGSHSPIQRE
                     DIRIEQVRRPARVDVCLLIDASASMAGRRIQAAKHLARHLFLTCRDKVSVLTFQDRKV
                     TQHVLRARSIRALEDGLATVRPAGLTPLAAGIVEAVKLLGSRKGVPTMLVLLTDGIPT
                     MNYWTGDPARDALVAAEQIARNKLPFTCIGLAPNRGFLRKLTEIANGTLYIVDEFDRD
                     LLAKLVKDERERVQTW"
BASE COUNT          791 a         1107 c          818 g          773 t
ORIGIN      
        1 cccagcttta gcttcaaatg ttttccgtcg ggccctacgt tcctccgctc cagaacatgc
       61 atgttccttg tgacaaagag tggagtcgga ttaccgtatc caaagggttc cagacgttcc
      121 agttccatcg cgagctcgat acagatctcg tcttcgccaa gctctgcatc gacaagcacc
      181 cttggaacga gatcctcctc tgtaagccat tcatgtccta tctcgttgag ccgttctctg
      241 aacaagtcca aatcttccgc ctttattgac aatcctgccg cctgtgaatg tcctccgtat
      301 ttgagcaaca gatcagaaca ttccgtcaga gccgaataca gattgaatgc aggtatactt
      361 ctggctgatc ccctgcccac gtctccctca aaggatatga ggaccgtagg cctgtacgtc
      421 agctcagcta ttctcgacgc aacgattcca ataaccccac tgtgccatcg gtcagagccc
      481 aacacgattg cgcgagtagt ctgtgtattc atcttgctca caggcaccca ggcctcccgc
      541 aagatggaga gctcgatctc ttgccttttg cggttttctt cgtcgagcat ggcggcgagc
      601 tctgcagcct cctgggccga atcggtcagg agcaacctgg ctcccaggct tggatcgcca
      661 agccttccgg ctgcattcac cctaggaccc accgtaaaac tgacgtgccc tgcccgaatc
      721 tcccggcctg aaagatccga tacgtccaaa agggcattga tgccgtgatt tgaagatcgc
      781 cccataagct ccagcccagc cttggctatt atcctgttct ccccctgtaa cggcaccaca
      841 tctgcaatgg tcccgagagc cacaatatcg agatacttgt gtgggtctgc cgcgcacggc
      901 ggccttcccg caagggtcca tacagcctga gcaagtttga acgccacgcc gactccggcc
      961 agttccttga agggataccg ggaatcgtgg cgctttgggt ctacaacacc aacacacgaa
     1021 ggcaggatac ttccagcttc gtggtggtcg gtgatgatca cgtctatgcc cagctcagag
     1081 gcataatcga cctcttcaat agcactgaca ccgcagtcta cggtcaccaa cagcgacacg
     1141 ccgtcgccag cagcctgctc tattgcttct ctgttaagcc cgtatccttc cacaagtcga
     1201 ttgggaatgt ggtagtccac attcgccccc aaagatctca agacggaaac aagcagcgca
     1261 acagaggtta tcccgtctac atcgtaatcc ccgtataccc gtattccctc accaatttcc
     1321 acggcagccc aaagcctagc agccgattgc tccatgtcag gcatagcgaa aggatcatgc
     1381 atttccgaag ggtcaggcat taggaaagcc ccaacagact tctcatcaac aatgcccctg
     1441 ttcgccaaaa cacgggcaag aacgggcgag atgccgcatg ttgccgctag ccttttttct
     1501 gctgccgagt ctccttcacg aagctcccat atcgttcttc taggggccat agatttctcc
     1561 accttaatca ggaaccattc gacttttgtc caagttcccc ttctagtcat gtacatgatt
     1621 caatactacc acgtttgaac cctttcgcgc tcgtccttta ccagctttgc cagcaagtcg
     1681 cggtcgaact catcaacaat gtacaaggtc ccgttggcga tctcggtgag cttccgcaga
     1741 aaccctcgat tgggagcaag acctatgcag gtaaacggca gcttgttcct ggcaatctgt
     1801 tccgccgcca ccagtgcatc tctagcaggg tcccccgtcc agtagttcat cgtgggtatg
     1861 ccatctgtaa gcaagacgag catggtcggt acccctttac gcgaccccaa aagcttcaca
     1921 gcctccacaa ttccagctgc gagcggagtc agccctgcag gcctcaccgt tgccaatccg
     1981 tcttcaagcg ccctgatcga ccttgcccgc aatacatgct gagttacctt cctgtcctga
     2041 aaagtgagga cactcacctt gtcgcggcaa gtcaagaaga ggtggcgggc gaggtgtttt
     2101 gcagcctgta tccgcctccc ggccatactt gcgctggcat ctatcagcag acacacatca
     2161 accctggccg gacgtctgac ctgctctata cggatatcct ccctttggat cgggctgtga
     2221 ctcccttccg ttgcgcaacg tacagcccaa ctccgcaccg tttcagcaac ggagatactg
     2281 ccccggtaat cgacctcaga cgcgggaatc acagtccgta caatacccgg tccgtttcca
     2341 gctctcatgc ccttggagaa atcgctccgc tccaggggcg tgccacgcgg cctggcgctc
     2401 cgacggatga tgcgacggac ctggttctcc acctctctgg cgaatttctt gagaaacaac
     2461 gcaagacgcc tgcccttagc tgtaaggaca agcgcagcgc cttctccctc cacgatttcc
     2521 aacgttctca gtttcgccag cattccctct gcgtcaccaa attcccgttc caacatggca
     2581 tagggattct ctcgcctgcc cggaccggcc acgcattcca gaaatgattt aaaactgttc
     2641 actgagccga aatcagtgca aacctccaaa gcttgcttca tcagatccgg taggctgttt
     2701 cctccatcat acgggatttg cccacgcatg taagaatcgg tcgaacacga ataaggagaa
     2761 agatcgaccg gcgaggactc ggaagaggaa gcattctcta tctgttccaa tcggcgcagg
     2821 ccaacaccgc tcgaaacgat tgatgcttca acctcacgga caatgtagta gaccaatcct
     2881 accatctcag gcgggaggaa tgccgcaacg tgcacgtgcc cgcggtgggc aatcttgagg
     2941 gactccttct gtccgaaatt gaggctgcct gtgacgcgcc cgccacctgt cgcggtctga
     3001 acatcgatga agtccgctag accagcgata gaccaaatcg gtccgcctaa ttccagcagg
     3061 ggcctaggga gtacagacat ctgctgacca taagcctcaa ccgcccgcct tatcgcttcc
     3121 gccgccgtac gatggtccac gtctgccaac tcatgaaaaa tgtctatatg aactacttca
     3181 tccgtcgaag accgctggta gaaaacctgt cccgcatcga ggcggcattg gacctgagcc
     3241 tgccccggac aatgcgggtc tagcacgtgg acacaccttg ataccaaagc cgtctcgcct
     3301 attctcacac cacgcccaag atctagctca ccgttgatct cacgggcaaa gctaagaaga
     3361 gcatcgatgt tcaagccaga tcatcctctg agaacacaag cttcgggcca atatcggtta
     3421 tgggccgcgc ggggtttgga gggcagacct cgctcaccga agccgacccg gccttagcac
     3481 gagttcccc
//
LOCUS       DAOSAI010000275         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17223_length_3485_cov_4.118950, whole genome shotgun sequence.
ACCESSION   DAOSAI010000275 DAOSAI010000000
VERSION     DAOSAI010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17223_length_3485_cov_4.118950"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PLM83_08760"
     CDS             <1..899
                     /locus_tag="PLM83_08760"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOK71528.1"
                     /translation="EELREAASRLQGKESRRKVARGESDDTTRVVAVTSGKGGVGKTS
                     FVVNLAMLLADFGMRVTILDADLGLANVDVLLGLAARHNLHHVIKGTLSLEDIIVTGP
                     RDVQIIPGASGLRDIADMAPEDRKRLIESLSRAVRGRDLLIIDTAAGMSRTVLDFVLS
                     AQEAIVITAPEPTAMTDAYAMIKVISRENPNAIVRIVVNMCHSRKEAENVVEQLSLLS
                     DRFLDFTFDALGYIPVDPSVSQAIRQRQPFVLAYPYSPASAAMESIARRLRGMPQVNE
                     GQAGVGQFIKRLLGVTAPDYLL"
     gene            910..1581
                     /locus_tag="PLM83_08765"
     CDS             910..1581
                     /locus_tag="PLM83_08765"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOK71529.1"
                     /translation="MDFPPLKPNARIEVRIKGYDESPKFATKVEGVSKSGYWIYAPYT
                     DDWRSMIRKGDEIEVAYPYRGAIYHYHSEVIGESGRRVKLLEISEPLFVRREQRREFV
                     RVPILLDVQYAPDCDDNGEVQWESGVAVDLSGGGTCIFLADPPSWLKCGETLNLILPI
                     GPDGGPFKVPAEIVRIEESDRPEQPGVSVGLSFIGITDREETAIVRFVFDRQRELIRK
                     AIDPE"
     gene            1588..2364
                     /locus_tag="PLM83_08770"
     CDS             1588..2364
                     /locus_tag="PLM83_08770"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOK71530.1"
                     /translation="MKDDFALWQEYGKTNSSAVREQLIVKYVHLVKHIVSRMPQAAIP
                     GVDYDDLIGYGVLGLIDAIERFDMSRGVKFETYAMTRIRGSIIDNLRRMDWAPRSVRH
                     KARQIECAMADAESVTGRPATDEEIADRLKVDIDTLHRWTWEISMASYSSLDELIAVD
                     DEHNVATLLDFVSDDGSPDPEMILEKDELYDALADAIDHLPEREGLVIKLYYFEEMTI
                     KEIARVLGVSESRVSQLHTKAMMRIRVEIMESRGCQCQPR"
     gene            complement(2722..>3485)
                     /locus_tag="PLM83_08775"
     CDS             complement(2722..>3485)
                     /locus_tag="PLM83_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK71531.1"
                     /translation="LWWVVASSLRPAGSLYQYATPISWRTFFPDELGIANFVNIFAEL
                     DFGQAMSNTLLVSTITVALGLLVNSMAGFALAKFDFPCKRLVFIVVLITFMAPFDTIV
                     IPLHQVISDLGMINTRAALILPAVGNGLVIFMFKQFYQEIPSELLDAARADGASWPRI
                     FWQVVMPISMPAVATAAVMMFLFQWNSLFWPLIAAFSPKYRTVQVGVATQISVDQTHW
                     ANLFASAVMGSLPPIILFLSLQKRYLKGISSLGND"
BASE COUNT          894 a          803 c         1022 g          766 t
ORIGIN      
        1 ccgaagaact gagagaggca gccagccgat tgcagggaaa ggaaagcagg cgaaaagtcg
       61 cacgcggtga atcagatgat acaacgagag ttgtggcggt tacatcagga aaaggcggcg
      121 tcggaaaaac cagcttcgtt gtgaaccttg ccatgctcct cgccgacttc ggcatgcgcg
      181 tgacaatact cgatgctgat cttgggctcg ccaatgtcga tgttctgctg gggctggctg
      241 cacgccacaa tctgcaccac gtgatcaagg gaacattgtc attggaagac atcattgtaa
      301 cgggcccgag ggatgtgcag ataatacctg gcgcatcagg actgcgtgac atcgcggaca
      361 tggcgccgga agatcgcaag cgcctcatag agtcgctttc cagagcagtg cgcggacgcg
      421 acctgctcat catcgatacc gcggccggca tgtccaggac tgtgctcgac tttgttctgt
      481 cagcccaaga ggcgatcgtc attacagctc ctgagccaac ggccatgaca gacgcttatg
      541 cgatgatcaa ggtgatatcc cgagaaaacc ccaatgccat tgtccgtatt gtggtcaaca
      601 tgtgccacag tcgaaaagag gctgaaaatg ttgtcgaaca gctcagtctc ttatcagaca
      661 gatttctcga tttcacattt gatgcgctag ggtacattcc ggttgaccct tcggtcagtc
      721 aggccataag acagcgtcaa ccgtttgtgt tggcttaccc gtacagccct gcttcggcag
      781 cgatggaatc gatcgcgcga cggctgcggg gcatgcccca agtgaacgaa ggccaggccg
      841 gagtggggca gttcatcaag agattgttag gcgttacagc tccggactat ctattgtgag
      901 ggggttgcca tggattttcc tcctctcaaa ccaaatgcga gaatagaagt aaggatcaag
      961 gggtatgatg agtcgcccaa atttgccacc aaggtagaag gggtctcgaa gtcaggatat
     1021 tggatatatg ctccgtatac ggatgattgg cgatctatga tccgcaaagg cgacgagatt
     1081 gaagttgcat atccttatag aggcgcgatc tatcattacc actcagaagt aataggcgaa
     1141 tcgggacgca gagtaaagct gctggagata tcggagccgt tgtttgtcag gcgcgagcag
     1201 cgacgggagt tcgtgagggt cccaatattg ctcgatgtcc agtacgcgcc tgactgcgac
     1261 gacaatggtg aggttcaatg ggagagcggt gtagctgtag acctaagtgg tggtgggaca
     1321 tgcattttcc ttgcagatcc tccgtcatgg ttgaaatgtg gtgagacact caacctcatc
     1381 ttgccgattg gaccagacgg cggccctttc aaagtgcctg ctgagattgt aagaattgag
     1441 gaatcagaca ggcctgaaca gcccggggtc agcgttggtt tgagcttcat cgggattacg
     1501 gatagagaag agacagcgat agtaaggttt gtttttgata ggcaaagaga gcttatcaga
     1561 aaagccattg acccggagtg atacagcatg aaagatgact tcgcgctgtg gcaggaatac
     1621 ggtaagacga atagtagcgc agtgcgggag cagcttatcg tgaagtacgt ccatctggtg
     1681 aagcacattg tttcacgcat gcctcaggcc gccataccag gcgtagacta cgatgatctc
     1741 ataggatatg gagtgctcgg tctcatagat gcgatagaga gattcgacat gtcccgagga
     1801 gtcaagttcg agacctatgc catgaccagg atacggggga gcatcattga caacctgaga
     1861 aggatggatt gggcgccacg atctgttcgt cacaaagcca ggcagataga gtgcgccatg
     1921 gccgatgccg aaagcgttac gggacggcct gcgactgatg aggagattgc ggacaggctg
     1981 aaggtagata ttgataccct ccataggtgg acgtgggaga tcagcatggc gtcatattca
     2041 tccctcgacg agcttatcgc cgttgatgat gaacacaacg tggctacgct ccttgacttc
     2101 gtgtctgacg atggcagccc ggatcctgag atgatcctgg aaaaagatga gctctatgac
     2161 gcccttgctg atgccatcga ccacctgcct gagcgagagg gtcttgtgat caagctgtac
     2221 tactttgagg aaatgacgat aaaggagata gccagggttc ttggcgtgtc agaatcgcga
     2281 gtatcccagc ttcatacaaa ggccatgatg aggattagag ttgaaataat ggagagccgg
     2341 ggttgccaat gtcagcccag ataatgccgg gaagggtgag gaggaggccg gcgcctacgg
     2401 agccggcaac aacaatcgac cggctgcacc catgccaatc tagaaatggc ttgcggtatg
     2461 gggggatctg tttggcaatc taaaagtcta tagtccggca gtcaaagctg tgaatagatt
     2521 ctgatgccca tgtcaataat atacgtgggc agcgatgttc gaacaaaaca gaataaccat
     2581 ctgagcgggg aaggcgcggt ctgcgattgg ggccgcgtct ttccttttat gatgctcccc
     2641 gactccttat tcctcactgt attcagaaag ccgttgacca ggccatgcca tgtggacgcg
     2701 aaatgcgagt ttgggggctg ctcagtcgtt gcccaaactg gagatgccct tcaggtagcg
     2761 tttttggagc gataggaaca ggattattgg cggaagactt cccatcacag ctgacgcaaa
     2821 gagatttgcc caatgggtct gatcgacaga gatttgcgtg gctactccaa cctgaactgt
     2881 tcgatactta ggggagaatg ccgcgatcaa cggccaaaag agtgaattcc attgaaacaa
     2941 gaacatcatc actgcggcag tggcgacagc aggcatcgag attggcatta cgacctgcca
     3001 gaagattcgc ggccatgatg ctccgtcagc gcgtgcggca tctagcaatt cggaaggtat
     3061 ctcttgatag aattgcttga acatgaatat gacaagtcca ttgcctacgg ctgggagtat
     3121 cagagctgcg cgggtgttta tcattcccag atcgctgatt acctgatgga gcggtattac
     3181 gatcgtgtcg aacggtgcca tgaacgtgat cagaacgaca atgaacacca accgtttgca
     3241 tgggaagtca aacttggcaa gggcgaatcc ggccattgag ttgacgagga ggccgagcgc
     3301 aacggttatg gtggatacaa gtagtgtgtt tgacatggct tgtccgaagt cgagttcggc
     3361 aaagatattg acgaagttcg caatgccgag ctcatcaggg aagaaggttc gccatgagat
     3421 cggcgtggcg tactgataaa gagaacctgc cggcctcaat gatgatgcga caacccacca
     3481 cagag
//
LOCUS       DAOSAI010000276         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17281_length_3475_cov_3.349123, whole genome shotgun sequence.
ACCESSION   DAOSAI010000276 DAOSAI010000000
VERSION     DAOSAI010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17281_length_3475_cov_3.349123"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLM83_08780"
     CDS             complement(<1..714)
                     /locus_tag="PLM83_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="MutS2; MutS-II; involved in blocking homologous and
                     homeologous recombination; has ATPase activity stimulated
                     by recombination intermediates; inhibits DNA strand
                     exchange; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOK71532.1"
                     /translation="MPRTHFGYVVRANATCGGVLMDDRSLRVLEYYKVRDMLEQCASC
                     ELGRERIRQLAPITNLAMIEEAQRGTSEARSLLDMGKQIPFGGVRDVRSSVERADRGA
                     TLSCDELLDILSTLSTSRSLKKILGSLGPEYPVLADAAADIGTFGDLEQAISRAISES
                     GEVMDSASSALRSIRSKIRSLNARVRDNLDSMVRSPQWSRLLQDPIVSIRNGRFVVPV
                     RAEMRSQVPGIVHDQSASGA"
     gene            complement(758..1315)
                     /locus_tag="PLM83_08785"
     CDS             complement(758..1315)
                     /locus_tag="PLM83_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71533.1"
                     /translation="MELSREFRERFVRSLQGRDYILYGGVLQLAKERGLRRISTRIIQ
                     IPSKDNSMYAVVEAEVETEDGVFSEVGDASPESVARSIQPHLRRMAATRAKARAMRDA
                     VGIDMVALEELGDALPDRDVMDEEVDLRSPEDLVIRFGKYSGKTLGEIYGVDRGYVEW
                     LSQNARDEAIRDASRELMLPSTPEM"
     gene            complement(1337..2053)
                     /locus_tag="PLM83_08790"
     CDS             complement(1337..2053)
                     /locus_tag="PLM83_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOK71534.1"
                     /translation="MEAKMFHAIVRSLTVYFFVLASMRLMGKREIGNISPFDLVVAIM
                     IAELGAIPLQSHDIPLGHGLVPIAILAAVEVLVALACLKFPGIRRLVIGEPTVIIEDG
                     KVMGRNMQKLRYNINDLLSQLRDKDVFNVADVEFALLEPSGRLSVLLKSQRRPVTPED
                     LSIETHYEGPSYPLVADGKIQYQYLKMLDLTVKDLTDMLKAKDIQSVEDVFFASLDSG
                     GELYVTMQDEVCLDELERGE"
     gene            complement(2139..>3475)
                     /gene="polX"
                     /locus_tag="PLM83_08795"
     CDS             complement(2139..>3475)
                     /gene="polX"
                     /locus_tag="PLM83_08795"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOK71535.1"
                     /translation="EKTEDKIIRAIENMKSTEERRPLYKVLPIAHDFEAMLGAVEGVE
                     RVGIGGSLRRRRDTVGDIDLIASTRAGDLVMEAFSASDMLREITAMGPFKAEAIAVSG
                     IRCEIRVVAPESYWTGFHHITGSRAHHVKLRRIAGRHGLKISEYGVRRDDTGEMIPVG
                     SEEELYAILGMDYIPPELREDWGEIEAALEGRLPDLIDEGAIRGDLHIHTNWSDGVAS
                     IEEMARAARDIGHEYIAITDHSRSLGIARGLSEDRLAEHIEAVRRVDAKIDGIRILSG
                     AEVDILKEGRLDYSDELLSKLDVVIASVHSGFQQDRDTMTRRIIEAMRNPNVDILAHP
                     TGRLLGRRPGYEVDIEAVIREAAATGTALEINSYPDRLDLRDEHARMARDRGVMISIN
                     TDSHSPEELENIRYGVWVGRRAWLERQDVLNAMTCEELLVWLGSHKRHMLPV"
BASE COUNT          787 a         1080 c          781 g          827 t
ORIGIN      
        1 agccccagaa gccgattggt catggactat gcccggaacc tgcgacctca tttcggcgcg
       61 aactgggaca acaaatcgcc catttctgat actcacaatt ggatcctgaa ggaggcgcga
      121 ccattgagga gatcttacca tcgaatctaa attatcacgc actcttgcat ttaacgagcg
      181 tatcttcgat ctaatgcttc tcagagcaga gctcgccgaa tccatcacct cgccggattc
      241 agatatcgcc cgcgagattg cctgctccaa atcgccgaat gtaccaatgt cagcagccgc
      301 atcagcaagc acagggtact ccggaccaag gctgcccaag atcttcttca ggcttctgct
      361 tgtcgacagc gttgacagga tatcaaggag ctcatcacag gagagagtag cgcctctatc
      421 ggccctctcc acactagaac gcacatcgcg cactccgcca aatggaatct gtttccccat
      481 gtcaagcagc gatctcgcct cgctagtccc ccgctgggct tcctcaatca tggcaagatt
      541 tgtaatcgga gccagctgcc ttatgcgctc cctaccaagc tcgcacgaag cgcactgctc
      601 caacatgtcg cgaactttgt aatactcgag aacacgcaat gaacggtcgt ccatgagaac
      661 tcccccacat gtcgcattgg cacggacgac atacccgaaa tgggtccggg gcattccgga
      721 cccaccgctc gccgcgctct gttcaattat agcagactca catctcaggt gtgctgggca
      781 acatcaactc cctagacgcg tctcttatgg cttcatccct cgcgttctga ctcagccatt
      841 ccacgtatcc gcgatcgact ccgtatatct ctcccagagt cttccctgag tattttccga
      901 acctaataac gagatcttcc ggacttcgca aatcaacctc ctcatccata acatccctat
      961 ctggcagagc atcccccagt tcctcgaggg caaccatgtc tatgcccacg gcgtccctca
     1021 tcgcccgcgc cttagccctg gtcgctgcca tcctgcgaag gtgcggctga atggatcggg
     1081 caacgctttc gggcgaagca tcgcccacct cggaaaagac tccatcctca gtttccactt
     1141 ccgcttccac cacggcatac atcgaattat ccttgctggg gatctgaatt atccgcgtgc
     1201 ttatgcgcct cagcccgcgc tccttagcta attgcaaaac gccgccgtaa aggatgtagt
     1261 ctcgtccctg caagctccgg acaaacctct cccgaaattc gcgagacagc tccatagctc
     1321 taccccctca cacttcctat tctccccgtt cgagctcgtc gaggcaaact tcgtcctgca
     1381 tagtcacgta cagttcgcct ccgctgtcga gagaggcaaa gaagacatct tcaacacttt
     1441 gtatgtcctt cgccttcagc atatccgtga ggtctttcac tgtcagatcg agcatcttca
     1501 ggtattgata ctgtattttc ccgtcagcta ccaatgggta agatggccct tcgtaatgag
     1561 tctcaattga caggtcttcg ggcgtcaccg gtctccgctg cgacttcaag agcacgctga
     1621 gcctaccaga aggctccaga agagcgaact ccacgtctgc cacgttgaat acgtccttgt
     1681 ccctgagttg gcttaggagg tcgttgatgt tgtatcggag tttctgcatg tttcgtccca
     1741 tcacttttcc atcctcaatt atcacagtag gctcgccaat cactaatctc cggattcccg
     1801 gaaacttgag gcaagcaagg gccaccaaca cttccacagc agcaagtata gctataggaa
     1861 caagaccgtg gccaagggga atatcatgac tctgaagtgg aatagcaccc aattccgcga
     1921 tcatgatggc aactacaaga tcgaaagggg atatattccc tatctcgcgt ttccccatca
     1981 gacgcatgga tgcgagaacg aagaagtaga ccgtaagtga tcggactatc gcgtgaaaca
     2041 tcttagcctc cacaaaaaaa gactcgcgca cggcgcatac attagtatgc ccatacgcga
     2101 attcgaggta ttccggacat ctcttcccag caaaacgctc agaccggcaa catgtgccgc
     2161 ttgtgtgaac caagccatac aagaagttcc tcgcacgtca tggcattgag cacgtcctgg
     2221 cgttcaagcc aggcgcgtct gcctacccaa acgccgtatc tgatgttctc cagctcttcc
     2281 ggagaatgcg agtcggtgtt gatggaaatc atcactcctc ggtctcgcgc catgcgggca
     2341 tgctcgtctc ggagatcgag ccggtctgga tatgagttga tctccaacgc agtcccggta
     2401 gctgcagctt ctcgtatcac ggcttctatg tccacctcat aacctggcct gcgtccaaga
     2461 agccgccctg ttgggtgcgc cagtatgtcg acattgggat tgcgcatggc ctcgatgatt
     2521 ctcctcgtca tcgtatcgcg atcctgctga aatccgctgt gaacagatgc gatgacgaca
     2581 tccaacttgg aaagaagctc atctgaatag tcgagccgcc cttcctttag tatgtcgact
     2641 tctgctccag aaagaatcct gatcccatca atcttggcat caactctgcg caccgcctca
     2701 atatgttctg ccagcctatc ctctgatagg ccgcgggcga tgcccaaaga tcgggaatgg
     2761 tcagtgattg caatgtactc atgccctatg tcccgagcag ctcttgccat ttcctctatc
     2821 gaagctacgc cgtcgctcca gttggtatgg atgtgaagat ctccgcgaat cgcgccttcg
     2881 tcaatgagat ccggaagcct tccctcgagt gcagcctcta tctcacccca atcctcccga
     2941 agctccggag ggatgtagtc catgcccaag atagcataaa gctcctcctc actgcccacg
     3001 gggatcatct cccccgtgtc atcgcggcgc actccatatt cgcttatctt taggccatgt
     3061 ctgccggcga ttcttctaag ctttacgtga tgcgctcttg atcctgttat gtgatgaaag
     3121 cctgtccagt atgactcagg ggcaacaacc cgtatctcac atctaatacc agaaaccgca
     3181 atcgcctcag ctttgaaagg ccccatcgcg gttatctccc tcagcatgtc gctggcggag
     3241 aaggcctcca taacgagatc tccggctcga gtcgacgcga taaggtcgat gtcacctacg
     3301 gtatcgcgcc ttcgccgcaa gctgccgccg atgccaactc gctcaactcc ttcgaccgcg
     3361 ccaagcatgg cttcgaaatc atgggcaatc ggcaatacct tgtatagagg tcttcgttcc
     3421 tcagtagatt tcatgttctc tattgccctt attatcttat cctccgtctt ctcgc
//
LOCUS       DAOSAI010000277         3474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17284_length_3474_cov_3.071073, whole genome shotgun sequence.
ACCESSION   DAOSAI010000277 DAOSAI010000000
VERSION     DAOSAI010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17284_length_3474_cov_3.071073"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PLM83_08800"
     CDS             <1..733
                     /locus_tag="PLM83_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOK71536.1"
                     /translation="AMPLPLQAKLLRVLQEGSLRRIGESSERKVDVRLISAMSSNPAT
                     AVERNLIRRDLFFRLGVIIIEIPPLRKRKDDIPALTEHFIERYSSQFGVNVHGVTRDV
                     FELFMEYPWPGNVRELQHAIEAAVQLSHGSLIGLEHLPPHVLSHGPGDAASRPRANRF
                     LPTSQFRDNSTPAIEEEAKAPLVPTLEQIEKSLIWAALETCEGNISATARYLDIPRQT
                     LQHKLKRYSIDASDIRRKDLDYTDK"
     gene            941..1981
                     /locus_tag="PLM83_08805"
     CDS             941..1981
                     /locus_tag="PLM83_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HOK71537.1"
                     /translation="MAKGCKYGTHRVIEPEGSMPQAAWKIDNEMAVRDNEILINVQTL
                     NIDAASFTQIKKQAGNDEKRIGEIMMGIVADRGKHHNPVTGSGGMLIGTVEKIGPDLI
                     GSTDLEVGDRIATLVSLTLTPLVIDKIIKVHLDTDQVDIEGHAILFESGIYAKLPNDM
                     PDSLSLAVLDVAGAPAQTAKLVHPGDTVLIIGAGGKSGLLCMYEAKKRAGVTGKVIAL
                     AHSEASKARAEATGFADVVIAGDATRPLEIMKMVEEATGGKMADVTINCVNIPGTEMS
                     SILSTRDMGIIYFFSMATSFTAAALGAEGVGRDVTMIIGNGYTRGHAEIALETLRESP
                     KLRKIFEDLYAR"
     gene            1971..3239
                     /gene="ablA"
                     /locus_tag="PLM83_08810"
     CDS             1971..3239
                     /gene="ablA"
                     /locus_tag="PLM83_08810"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOK71538.1"
                     /translation="MQDNDGDDVSMWEGVSKEQWNDWHWQFGNRITTVDQLKSVVNLT
                     PEEEEGVQRCLARLRMAITPHYASLIDPDDPDCPVRKQAIPVVQELSMGEHDLMDPLD
                     EDVDSPVPHVTHRYPDRVLFLVTSVCSTYCRHCTRRRFAGHTDSAITHDELIQGIQYI
                     RETPEIRDVLISGGDPLTLPDDTLEYIISELRAIPHVEIVRIGTRTPVVMPQRITPEL
                     CNMLKKYHPLWLNTHFNHPKEITPESRRACEMLADAGIPLGNQTVLLKGVNDCPHIMK
                     KLVHELVKIRVRPYYIYQCDLSMGIEHFRTSVGKGIEIVERLRGHTSGFAVPTFVIDA
                     PGGGGKIPIAPQYVISQTDTKVILRNYEGVITAYPEPEDKISQCHQCGICEEIPKPAT
                     EGVAAVMDGMVSSLEPVGLERRKRSRRDPS"
     gene            3187..>3474
                     /locus_tag="PLM83_08815"
     CDS             3187..>3474
                     /locus_tag="PLM83_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71539.1"
                     /translation="MNQLDSSVARDPGEILLNLALGAPGKCVGIVGLAKNCGKTTVLN
                     RMIRQATSNGITVGVTSGGRDGELYDAITGLPKPPIYLPAGSLVALAESTIH"
BASE COUNT          912 a          911 c          942 g          709 t
ORIGIN      
        1 cgcaatgcct ctgccgctcc aggcgaagct tctcagggtg ctgcaggagg gctcattgcg
       61 caggataggg gagtcttctg agcgaaaggt agacgtgcgc ctaatatccg ccatgtcctc
      121 gaatcccgcc acagcagtag aacgcaatct gatacgacgc gacctcttct tcaggctggg
      181 cgtcatcatc atagagatac ctcctctgag gaagcgaaaa gacgatatac ctgcgcttac
      241 cgaacacttc attgagcgct acagctcaca atttggcgtg aacgtgcacg gagtaacccg
      301 tgatgtattt gaactgttca tggaatatcc gtggcctgga aatgtgagag agcttcaaca
      361 cgcaattgaa gcagcagtcc agttgtccca cggatcactc ataggactag aacatcttcc
      421 accgcatgtt ctttcgcacg gcccaggaga tgcggcatcc cgacctagag ccaatagatt
      481 cctgcccacc agccagttcc gagataactc aacgccggca atagaggaag aggccaaggc
      541 gccgctggta cccacccttg aacagatcga gaagagcctg atatgggctg cactcgaaac
      601 atgcgaggga aacatctctg ccacagcccg atacctcgac atacccaggc agactctgca
      661 gcacaagctg aagcgttaca gcatcgacgc ttccgacata cgcagaaaag atctcgatta
      721 tacagacaaa taagtaacgg tccgtcattg gagcggccgg ggcagttcta tatgtcactt
      781 gaagtggcga actgtcacgc acgcagtgcc cataattggg cagctgcccg taaatgagca
      841 gacgttggac aggggcaagc gcagctacag cgtgtttcgt ctctggcatg gatattgcaa
      901 cgttatctct tgtgtgcgtg tatcatagga ggtgtggcaa ttggcaaaag gctgtaagta
      961 tggaacccac cgagtaatcg aaccagaggg ctcgatgccg caggctgcat ggaagatcga
     1021 caatgagatg gcagtccgcg ataacgagat cctcattaat gttcaaaccc tcaatatcga
     1081 cgctgccagc ttcacccaga tcaagaagca ggccggaaat gacgagaaac ggataggcga
     1141 aatcatgatg ggcatcgtcg ctgaccgagg gaagcaccac aacccagtta ctggttcagg
     1201 aggaatgctg ataggaactg ttgagaagat cggacccgat cttattggca gtacggatct
     1261 tgaggttgga gacaggatcg ctaccctcgt ctctctcact ctgactcccc ttgtcatcga
     1321 caaaatcatc aaagttcatc ttgataccga ccaagttgac attgaagggc atgcaatcct
     1381 tttcgaaagc ggaatctatg ccaagcttcc gaatgacatg ccagatagcc tctcgttggc
     1441 agttttggat gtggcgggag cccccgcaca gaccgcaaag ctagtgcatc ctggcgatac
     1501 cgtgctcatt atcggggcag gcggtaaatc cggccttctc tgcatgtatg aggccaagaa
     1561 acgtgccggc gtcaccggaa aggtaatagc attggcacac agcgaagcca gtaaagcccg
     1621 ggccgaggca acaggatttg ccgatgttgt gattgctggc gatgctacgc ggccgctgga
     1681 gatcatgaag atggttgaag aggcgacagg cggaaaaatg gcagacgtca cgatcaactg
     1741 cgttaacatt ccgggcacag agatgtcgtc catactgtca acacgcgata tgggcatcat
     1801 atatttcttc agcatggcga cgagcttcac cgccgcggcc ctaggcgcag agggagtcgg
     1861 ccgtgacgtc acgatgatca tagggaacgg ctatacccgc ggacacgcgg aaattgccct
     1921 tgagactctg cgtgagagcc cgaaacttcg caagatattc gaggatctct atgcaagata
     1981 acgacggaga tgatgtcagt atgtgggaag gagtttccaa ggaacagtgg aacgattggc
     2041 actggcagtt cggaaacaga ataaccactg ttgaccagct caagtcggta gtcaatctca
     2101 cccctgaaga ggaagaaggg gtgcagcgct gccttgccag gttacgcatg gcaataactc
     2161 cgcactacgc gagtttaatt gatcctgacg accctgattg tccagttcga aaacaggcaa
     2221 ttccagtcgt ccaggagctt tccatgggag agcatgatct catggatcca cttgacgaag
     2281 atgtggattc ccctgtgcct cacgtaactc acagatatcc cgatagggtt ctgttccttg
     2341 tcacgtccgt ttgttcaacc tactgtaggc attgcaccag gaggaggttt gccgggcata
     2401 ccgacagcgc cataacccat gacgaattga tccagggcat ccagtacata agggagacgc
     2461 ctgaaataag ggatgtcctg atttcagggg gagatccgct caccctacca gatgacaccc
     2521 tagagtacat cataagcgag ctgagggcga tcccccacgt ggagatagtc aggatcggca
     2581 caagaacccc ggtggtcatg ccgcagcgca taacgccaga gctctgcaat atgctcaaga
     2641 agtaccatcc cctgtggctc aatacccatt tcaatcatcc gaaggagata acgcctgagt
     2701 cacgcagagc gtgcgagatg ttggctgacg caggcatacc ccttgggaat cagacagttc
     2761 tgctcaaggg agtgaacgat tgcccccata taatgaagaa gctcgtgcac gaacttgtga
     2821 agatcagagt acgcccttac tatatctatc aatgcgatct atcgatgggg atagagcact
     2881 tcaggacatc ggtaggcaaa gggatagaga tcgtagagcg tttgaggggc catacatcgg
     2941 gattcgcggt tcccactttc gtgatagatg ccccgggagg gggaggaaag atccccatcg
     3001 ccccgcaata tgtcatctcg cagaccgaca ccaaagtaat tcttcgaaac tacgaaggcg
     3061 tcatcaccgc ttatcccgaa cccgaagaca agatcagcca gtgccatcag tgcggcatct
     3121 gcgaagagat tccgaagccc gctacagagg gcgtcgcggc tgttatggat gggatggtgt
     3181 caagccttga accagttgga ctcgagcgtc gcaagagatc caggagagat ccttcttaac
     3241 cttgctctgg gggccccggg caaatgcgtc ggcatagtcg gccttgcaaa gaattgcggc
     3301 aaaacgaccg tgctcaacag gatgatccga caggcgacat ccaacggcat cacggtaggc
     3361 gtcacgtccg gcgggcgaga tggcgaactg tatgatgcca taacaggact tccgaaaccc
     3421 ccaatatatc tgcccgcggg atctctcgtt gctcttgccg aatctacaat tcat
//
LOCUS       DAOSAI010000278         3467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17319_length_3467_cov_4.051290, whole genome shotgun sequence.
ACCESSION   DAOSAI010000278 DAOSAI010000000
VERSION     DAOSAI010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17319_length_3467_cov_4.051290"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PLM83_08820"
     CDS             complement(<1..162)
                     /locus_tag="PLM83_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOK71540.1"
                     /translation="MSELRKTPLFEAHESLGGKIIDFGGWALPVQYSGILDEHRAVRE
                     RAGLFDVSHM"
     gene            430..1803
                     /gene="lpdA"
                     /locus_tag="PLM83_08825"
     CDS             430..1803
                     /gene="lpdA"
                     /locus_tag="PLM83_08825"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOK71541.1"
                     /translation="MYDVVIIGGGPGGYVCAIRAAQNGLSVALVEKDKLGGTCLNRGC
                     IPTKALAHTAEVMDSAQRASELGLGIPEVGVDFPRVMAKKEKVVAKLRGGVNLLIKRR
                     KVDFVSGVGTLAGTRAVEVVAPDGSVSRLEARNIVIATGSEPITPRIFGYDGVNVLTS
                     EDVLKLSALPKSLLIIGAGVIGCEFASIFRTFGCDVTLVDIMPNILPMVDDDVADVVR
                     KSFKKRGIDIHTGTKIASVNIVNGQVEATTEDGSVFRAEKAILSIGRRPSTGGVGAPD
                     VGIDVGPAGEIIVDSKMRTNVPGVWAIGDVSNKAQLAHVASAQGLVVAANIAGEKCNM
                     EYFAVPNCIFTSPEVAAVGVSERGAQEACIDYKVGKFPFVASGKAMAMGETDGFVKVI
                     SDGSGRVIGGQIVGPHASDLIAELALAVSRQLTLEDIASTIHAHPTLAEAVCEAAEAA
                     MGRAIHI"
     gene            2142..2633
                     /locus_tag="PLM83_08830"
     CDS             2142..2633
                     /locus_tag="PLM83_08830"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOK71542.1"
                     /translation="MSNRTLAGIALVILGTLLLMSNFGIRARFPAASFWPLLLALVGA
                     MFIRDALRPGGERSHVFVGVIILGIGIMYFLKAIGLIRIGMGKLWPVYLVIVGIAFFA
                     QAAAMNVPHYSGTGALLCGVGIILLAFNLRLVDYGIFREAVKYWPVLIIAAGVLKLVD
                     SYR"
     gene            complement(3262..>3467)
                     /locus_tag="PLM83_08835"
     CDS             complement(3262..>3467)
                     /locus_tag="PLM83_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71543.1"
                     /translation="TGTTSYADPSIGHADIDDPLMGNWQAITICCKSRPFSRPWPLAP
                     QAIGARHYSLSGRSFPMPAADRF"
BASE COUNT          742 a          815 c         1053 g          857 t
ORIGIN      
        1 catgtgcgac acatcgaaaa gcccggctcg ttcgcgcact gcccggtgtt catccaagat
       61 gccactgtac tgaacgggca gcgcccaacc gccgaaatca attatctttc cgcccagcga
      121 ttcgtgggcc tcgaatagtg gggtcttgcg cagttctgac attactccac ctcctgccag
      181 ataagtatta tgcgccaatt gaaggaatgc ttgcgcccag ctgacggcta tcccaatggc
      241 gctggcatat tcatgcttgg tattcatcac ttgcgggcaa actccttcta gtcgagtcgt
      301 cgggctgagt ccgttcggac gcgcactggc ccggtggcag gaacagctgc tcggaaaagc
      361 gaactatact cggactgctg ctgtattttt cccaatggcg gcagccatga tttagggagg
      421 attaatggaa tgtacgatgt tgtgataatt gggggcggcc ccggaggata tgtctgcgca
      481 atcagagcgg cgcagaacgg cctttctgtt gctcttgtcg aaaaagacaa gctcggaggg
      541 acttgcctca accgaggctg cattccaact aaagccttgg cccatacggc cgaagtaatg
      601 gattcggcac agcgcgcgtc agaattgggt ttgggcattc ctgaagtggg cgtcgacttt
      661 ccgcgagtta tggccaaaaa ggaaaaggtc gtcgccaaat tgcgcggcgg ggtcaatctc
      721 cttatcaaga ggcgcaaggt ggatttcgtg agcggcgtgg ggacgctggc tggaacgcga
      781 gcggttgagg ttgtggctcc cgacggttca gtatcgcggc ttgaagcgag gaatatcgtt
      841 atcgcgaccg gatcagaacc tattacgccc cgcattttcg ggtatgacgg cgtcaacgtg
      901 cttactagtg aggatgtcct caagctttcg gctcttccga agagtctcct tattataggg
      961 gcgggagtca taggatgcga atttgcgagc atcttccgga ctttcgggtg cgatgtgact
     1021 ttagtcgata tcatgccaaa catacttccc atggtcgacg atgatgttgc cgatgtggtt
     1081 cgcaaatcct tcaagaagcg tggcatcgat atacataccg gtacgaagat agcttctgtc
     1141 aatatcgtga atgggcaggt tgaagcgacc actgaagacg gaagcgtttt ccgggctgaa
     1201 aaggctattc tgtcgattgg caggcgtccg tctacaggcg gggtgggtgc tcccgatgtc
     1261 gggatcgacg tgggacctgc cggagagatc atagtcgact ccaagatgag gacgaacgtg
     1321 ccgggcgtat gggccattgg ggatgtctca aacaaagctc agctggcaca tgtggcgtct
     1381 gctcagggat tggtggttgc tgcgaatatc gccggggaaa agtgcaatat ggaatacttc
     1441 gcggtcccca attgcatctt tacgtcgccg gaggttgcgg ctgtcggagt gtcggagcgg
     1501 ggggcgcagg aagcatgtat tgactataaa gtaggcaaat tcccgtttgt cgcatctggc
     1561 aaggcaatgg ccatgggcga gactgatggc tttgtcaaag tcatctcaga tgggtctggc
     1621 cgagtcattg gcggacagat tgtggggccc catgcatctg acctcattgc cgagctggcg
     1681 cttgcagtct caagacagct gacattagag gatattgcca gcaccataca tgctcatcca
     1741 acgttggctg aggcggtctg cgaggcagcc gaggcggcaa tgggacgggc aattcatatc
     1801 tgagcaggtt atgagggagc attcggcgcc atcagtcgtt tgatatcgga acttcggccc
     1861 acgtgaattt gggtgtggcg caaagaaaag tcaatacatg agtagtcgtg acaggttctt
     1921 gcgcgatcct aagcctgcag ttcggatctt ggctgtctac tgaaggccgt gattcctttt
     1981 gcatcttgct tggcagccta gatgcttctc gaagcttgct cagttagtat tgttgcgggc
     2041 agtcgcggca cgtggatata gggctaggaa aacggggctg caatctgcat gtgacggggc
     2101 gcacatctta gagcgctttg tgaaaggagg cgggaacggt catgagcaat agaacactgg
     2161 caggaattgc gttggtcatc ctgggaacgc ttcttctcat gtcgaatttt gggataaggg
     2221 cgcgatttcc ggcagccagc ttctggccgc tcctcttagc tttggttgga gcgatgttca
     2281 tcagggacgc tctcagacca ggcggcgaaa ggagccatgt ctttgtcgga gtcataatac
     2341 tcggcatcgg gatcatgtat tttctgaaag caatagggtt gatcaggatt ggcatgggga
     2401 agctctggcc ggtctacctg gtaatagtgg gaattgcgtt ctttgcacag gcggctgcga
     2461 tgaatgtgcc gcattattcg gggacaggtg ctcttttgtg cggagtaggc atcattctcc
     2521 tcgctttcaa cttacgcctg gtggactacg ggatattccg cgaggcggtg aaatactggc
     2581 ctgttctgat aatagccgcg ggagttctca aacttgtcga cagctacaga tgatgatatg
     2641 agcggaagtc tcacagtgcg catgttgatg gcctgagacg gaaacgaaat gaattgcgcc
     2701 gacgagtatg ggcgcagagt gtcgttatag atcctaatcc acgcggggcg gggcttgcat
     2761 gatctcttgt cagagatcgg caggccttgt tttcattatc caagtgtagc ttttgacatg
     2821 acgccggttc ttttgagcga gtatcgatag gcttttctgc cattatccag tctgcattgc
     2881 tcgtagctgg ggctcttgcg gctcgattgg gccaagatgc tttttgcgag ttgccctcag
     2941 agcacagggc ttaagtatat tgtattggca actgtcggct ttatgcgaaa tgcataatct
     3001 cgaacgactg cggtccagta aagggttgag cgaaccttgc gatgtcgcaa tttacacgtg
     3061 gaatagcata ccgcaggccc tccagcatgc ttcagccgga atgtgctctt cttttgttgt
     3121 catttctatg cagcttcctg tacggatcta catggttcgc gcgatcggcg tgctacgatg
     3181 atctatggga atgcccgcca tggtggtaga cacggttttt ctctgcgagc cgatgtcggg
     3241 ttttccatgg tctgggtagg atcagaagcg gtcagccgca ggcattggaa aagaccttcc
     3301 agagaggcta taatggcgcg ctccgatcgc ctgtggagcc agcggccaag gacggctgaa
     3361 cgggcgggat ttgcaacaga ttgtaatggc ctgccagttg cccattagag gatcgtcaat
     3421 atcagcgtgc ccaatcgacg ggtctgcata cgaggtggtt ccagtcg
//
LOCUS       DAOSAI010000279         3467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17320_length_3467_cov_3.655041, whole genome shotgun sequence.
ACCESSION   DAOSAI010000279 DAOSAI010000000
VERSION     DAOSAI010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17320_length_3467_cov_3.655041"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLM83_08840"
     CDS             complement(<1..212)
                     /locus_tag="PLM83_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71544.1"
                     /translation="MEDVVPSGGSVKKRCALWGRLIMVCLLGIALIMCNMASANAAPS
                     GEEIAAKVTEKIKGVAALQADIASEFS"
     gene            complement(287..1045)
                     /locus_tag="PLM83_08845"
     CDS             complement(287..1045)
                     /locus_tag="PLM83_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HOK71545.1"
                     /translation="MGKKNHLPKQYHTKIWRAIYEFGLIEPDDRILVGLSGGKDSLFL
                     TYALKIIQSYSAIPFTFEAATVDIGFSDLFDTARIEEQCASLGVGCHIIGTHIADIIE
                     ERRKDENPCAVCSYFRRAALNRYASEHGFNKVALAHHQDDAMETFLMNILYSGRVYAL
                     PWKTYLSRTGITVIRPMMYLSEKDVVKGFKRTGLEVVEAFCPYSGNTTRAKVKQLILD
                     LAKENRKVRPNLAAAMRDGDDVELWPPIVRHNRS"
     gene            complement(1123..1213)
                     /locus_tag="PLM83_08850"
     tRNA            complement(1123..1213)
                     /locus_tag="PLM83_08850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1177..1179),aa:Ser,seq:gct)
     gene            complement(1267..1355)
                     /locus_tag="PLM83_08855"
     tRNA            complement(1267..1355)
                     /locus_tag="PLM83_08855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1319..1321),aa:Ser,seq:tga)
     gene            1596..2633
                     /locus_tag="PLM83_08860"
     CDS             1596..2633
                     /locus_tag="PLM83_08860"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOK71546.1"
                     /translation="MRYISIDEAKPGMILARHIWGPHGQLLLTANTTLKETYIKRLKE
                     FRFSALYVKSYPDEPIENLVGPVKQETLVHARETLKAVTESVIASNRIDFAIVRETVE
                     DIVDQVLSNPNVVYSMADIKAFDDYTYGHSVDVCVLSILCGAHMGMNRYELVDLGSGA
                     MLHDVGKLFTPKEILAKPEPLNADEMEVIHRHPWDGFQVLRKHMPLIPAHVAFQHHER
                     YDGSGYPRGLLDADTLDLAKVVAVADSYEAMTSDRPYREAMMPHQALAELTRDAGKSY
                     NPTVIKCFVEVVAAYPLGTVVRLSDGSLATVVGVTKEIYDVQIVSGQSKGKTISIQRD
                     SNDISIVERLQ"
     gene            complement(2654..>3467)
                     /gene="serS"
                     /locus_tag="PLM83_08865"
     CDS             complement(2654..>3467)
                     /gene="serS"
                     /locus_tag="PLM83_08865"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOK71547.1"
                     /translation="SKITGSRFVIYKGAGSKLVRALINFMLDLHTQEHGYTEVFPPFL
                     INRDSAVGTGQLPKFAEDMFKCEGLDYYLAPTAEVPVTNMYRGEILPGDLLPIKHCAY
                     TACFRSEAGSAGRDTRGLIRQHQFDKVELVKFVHPDTSYDELERLVNDAESILQLLNL
                     PYQVTMMCTGDVGFSAAKKYDLEVWMPSYDRYVEISSCSNFEAFQARRADIRFRPAPG
                     ERAEFVHTLNGSGLAVGRTIAAMLENWQQPDGSVTIPPRLRGYMGKMEAITV"
BASE COUNT          895 a          974 c          822 g          776 t
ORIGIN      
        1 gaaaactctg acgcgatatc cgcttgaagc gcggcaactc ccttgatctt ctctgtcact
       61 tttgcagcga tctcttcgcc tgaaggagca gcattggccg acgccatatt gcacataata
      121 agcgcgatgc cgagcaggca aaccataata agcctacccc ataaagcgca ccgcttcttc
      181 acacttcccc cactaggcac aacatcttcc acgatactgc cccccatccc tgcaaataga
      241 cgaccacact tattataggc atctcagaaa acccgtgtca acaaagctag ctcctgttat
      301 gacgcacaat tggcggccaa agttccacat catcgccatc gcgcatagct gccgccaaat
      361 tcggacggac ttttcgattt tccttcgcaa gatcgaggat gagctgtttt accttggcac
      421 gtgtcgtgtt cccggaatac ggacagaaag cctcgacaac ttcgagtccg gtacgcttaa
      481 accctttgac tacatccttt tcggaaagat acatcatagg ccgtatgacg gttatccctg
      541 tgcgggacag gtatgttttc catggaaggg catacacgcg cccagagtag aggatgttca
      601 tcagaaacgt ctccatcgca tcgtcttgat gatgagccaa tgccacctta ttgaagccat
      661 gttccgacgc gtagcgattg agagcggccc tgcgaaaata agaacatact gcacatggat
      721 tctcgtcctt ccggcgttcc tctattatgt cggcaatatg ggtaccgatg atatggcagc
      781 ccacacccaa cgatgcgcat tgttcctcaa tgcgtgcggt gtcaaacaag tcggagaaac
      841 ctatatctac cgtggcggcc tcaaatgtga agggaatggc ggagtaagac tgaattatct
      901 tcaaagcata tgtaaggaac aagctatcct tgccgcctga aaggcccaca aggattcgat
      961 catccggctc aatcagtccg aattcgtaga tggcccgcca gatctttgtg tggtattgct
     1021 ttggaagatg attctttttg cccatgtctc gcccccccag cgtccaaaca aaaggggcat
     1081 gacctcctat tccgagccgg tcatgcctca taagaataaa actggcggag agagggggat
     1141 tcgaaccccc gctagggttt cccctactaa tggtttagca aaccatcccc ttcagccact
     1201 tgggtatctc tccgcacagg ctgatacatg agtcaaaaac aactatccat cgcgatttgc
     1261 gaattatggc ggagggggtg ggattcgaac ccacggccca tttctgagac accggttttc
     1321 aagaccggct ccataaaccg ctcggacacc cctcccatca agtgcctcca tagtatatca
     1381 cccatgtcga cgcccagtca agacactgtc cgcctccggg tttgcccaag aatgggaggc
     1441 cgaagcacca gcaagaagcc acgcgttctc cagacgctaa gcccaattgc tgcagcagga
     1501 tgttgcagct ctttgtggaa agtattgata gatcacgacg agcaatagca tagcagccga
     1561 aatcgataaa atgcaattga caacggggga tagcaatgcg ctacatttcc atagatgaag
     1621 ccaagcccgg aatgatccta gccaggcaca tatggggccc gcatggccag cttctgctca
     1681 ctgccaacac tacactgaaa gagacttaca taaaaagact gaaggaattc cgtttttcag
     1741 ctctctatgt aaagagctat cctgacgaac ccatcgagaa tcttgtaggt cccgtaaagc
     1801 aggagaccct cgtgcacgcc agagagacac tgaaagctgt caccgaatct gtcatcgctt
     1861 caaaccgtat cgactttgcc atcgtgcgtg aaaccgtcga agacatcgtt gaccaagtgc
     1921 ttagcaatcc aaacgtagtc tacagcatgg ccgatatcaa ggccttcgac gattacacat
     1981 atggacactc agtcgatgtc tgcgtcctgt cgatcctttg cggcgcccac atgggcatga
     2041 acagatacga attggtggac cttggatccg gcgcaatgct ccacgatgtg ggaaagctgt
     2101 ttacacccaa agagatactg gcaaaacctg agccacttaa cgctgacgaa atggaggtca
     2161 tccataggca cccttgggac ggattccaag tgttgcgcaa gcatatgcca ctcatccccg
     2221 ctcatgtggc tttccaacat catgaaaggt acgatggctc gggctatccc cgcggacttc
     2281 tagacgctga caccctggac cttgcaaaag tcgtcgccgt cgccgattcg tacgaagcca
     2341 tgacctctga caggccctac agggaagcga tgatgccaca ccaagccctg gcagaattga
     2401 cgcgcgatgc aggcaaaagc tataacccga cggtaatcaa gtgcttcgtc gaagtggtgg
     2461 ccgcctaccc gcttggcact gtagtaaggc taagcgacgg ttcattggcc accgttgtgg
     2521 gggtcaccaa ggagatctac gacgttcaga tagtctcagg ccaaagcaaa gggaagacta
     2581 tctcaatcca gcgcgattca aatgacataa gtatagtcga acgcctccaa taacccccgt
     2641 acaaccaagc cgtttacact gtgattgcct ccattttgcc catgtaacct ctgagccgag
     2701 gcggaatcgt cacagagcca tccggttgct gccagttttc caacatagcg gcgatcgtcc
     2761 ttcctactgc gaggccagag ccgttcaatg tatggacgaa ttcggcgcgt tcgccaggcg
     2821 caggcctgaa cctgatatct gccctgcgag cctggaatgc ctcgaaattg ctacatgacg
     2881 agatctcaac atacctatca tagcttggca tccatacttc gagatcgtac ttcttggctg
     2941 cagaaaaacc cacatctccg gtgcacatca ttgtgacctg atatggcaaa ttcaacaact
     3001 gcagaatgct ttcagcgtca tttacaagcc tctcgagctc gtcataagag gtatcaggat
     3061 gaacgaattt gaccagttcc accttgtcga actgatgctg gcgtatgagt cccctcgtgt
     3121 cgcggcccgc agagcctgct tctgaacgga aacacgcagt gtaagcgcaa tgctttatcg
     3181 gcaacaaatc gccgggcaag atctctccgc ggtacatgtt cgtcacagga acctccgccg
     3241 tgggcgctag gtagtagtca aggccttcac atttgaacat gtcctctgca aacttgggca
     3301 actggccagt cccaaccgct gaatcccggt taatgaggaa cggtggaaag acttcagtat
     3361 agccatgttc ttgtgtatgc aagtccagca tgaagttgat tagagccctt accagcttgg
     3421 agccggcgcc cttgtatatg acgaacctcg acccggtaat cttcgat
//
LOCUS       DAOSAI010000280         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17343_length_3464_cov_2.947199, whole genome shotgun sequence.
ACCESSION   DAOSAI010000280 DAOSAI010000000
VERSION     DAOSAI010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17343_length_3464_cov_2.947199"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLM83_08870"
     CDS             <1..470
                     /locus_tag="PLM83_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71548.1"
                     /translation="CQRREQRRSEHGKLDDGRSIGATVGTAISGFDLTAGHKVTQTMG
                     EKIETHNTTTLGIAMKEREVLPNMLISASYNAAMPDFSLAETRHIAKASVDADVSVLK
                     GIKVSALYDTKPATGKPGRELGIEYGAPCGVKLGYSCNDLNTNMVYAGMEVEF"
     gene            complement(950..2188)
                     /locus_tag="PLM83_08875"
     CDS             complement(950..2188)
                     /locus_tag="PLM83_08875"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOK71549.1"
                     /translation="MDERKPLVLMRGMIAIPVGNLQEQLAFKGFPLGEVDNVFGIRTE
                     LAVKQFQASVGLPETGIVDAQTWRLLFDGLPLPDEVFCPDDSLRYDNTVDYRGYSDRS
                     SASRMCISADKDKSTNGGSELALSASSKSRQGPSIHVSLSQRRLTLLQSERESAQYPV
                     AIGKPATPTPVGNWSILNKALNPGGVFGTRWMGFTRQGHGIHGTNQPQSIGHAVSNGC
                     IRMYTPDVEALFTQVSVGTPVVIDAGVPFPTPAPGTYIVRSGDTLYLIARRFGTTVEA
                     IRRTNNLTTDIISVGQVLFLPGPTGAPPIIGTDYVVQPGDTLFLIARRFGTTVQAIMS
                     ANNLTSTNIYVGQRLRIPGGPRTAAQMRAQSGASEYVVQPGDTLHSISAAFGIPVEAI
                     IAANTMISSDIYPGQLLRMP"
     gene            2333..3451
                     /gene="ugpC"
                     /locus_tag="PLM83_08880"
     CDS             2333..3451
                     /gene="ugpC"
                     /locus_tag="PLM83_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOK71550.1"
                     /translation="MAGVTLEHVTKKFGNVVAVNDADLEIKDKEFLVLVGPSGCGKST
                     TLRMVAGLEEITSGNIYIGDTLVNDIPPKDRDIAMVFQNYALYPHMDVYNNMAFGLKL
                     RKFPKEQIDERVKDAARILGIENLLDRKPKALSGGQRQRVAVGRAIVREPKVFLMDEP
                     LSNLDAKLRVQMRSELSKLHNRLQTTIIYVTHDQTEAMTMGDRIVVMKDGFIKQVGAP
                     LEIYQYPDNMFVAGFIGSPAMNFIPGVLRKKGDGYIMDCESFQITVPGNRFKDLGKYI
                     DERMVLGIRPENIQDADFVEDAPADRTVKCMVDVVEPMGAEAYLYLTVGPHSFVARVN
                     PRTTARDGEPHAVVLNMEECHLFEASTENAIAIKYTWG"
BASE COUNT          804 a          872 c          985 g          803 t
ORIGIN      
        1 attgccagcg gcgcgagcag cgccgcagcg agcacggcaa gctcgatgac gggcgttcca
       61 tcggcgccac ggtaggaact gctatctcgg ggttcgatct cacagccggc cacaaggtga
      121 cacagaccat gggagagaag atcgagaccc acaacaccac tacgctcggc atagccatga
      181 aagagcgcga ggtgctgccc aatatgctga taagcgccag ctataatgca gcgatgcctg
      241 atttttcact tgcggaaacc aggcatattg cgaaagcctc tgtggacgca gatgtttcag
      301 tgttgaaggg cataaaggtg tcggcactgt acgatacgaa gcctgcgact ggaaagcctg
      361 ggcgcgagtt gggtattgag tatggcgcgc cttgcggtgt caagctcggc tactcgtgca
      421 acgacctcaa caccaacatg gtctacgccg gtatggaagt ggagttctag tatcgatcac
      481 cggcaagctt cgtgatccgc ggactgcgcg gaatcactga agaatcgagt aggggggcgg
      541 tctgtccgcc ctcctccctc gccggataag cttgagcagt ctcttgtcca tcgctctgcg
      601 aaatcaacat ccgtatttac aacccatgtg tatccctttg cttttggccc gttcaatggc
      661 gcggaagccg tagcttgaat ctgaggactc cgggctgaag gcggatgtaa gaatctagtg
      721 aataggctgg tccaaaaccg ttgggacgcc aaacagcata aaactcctgt agtctctcca
      781 gagtcattcc atccactttg ggagtgctgc aaagatgttc tgctgatcta caatcaatcc
      841 catcagcgga tgccctgctt gtccgcggga gtgctcgtcg ggttgtgccg aagaaacgct
      901 ctgcttcccc ataccattgc gaactcaccg tatcactcgt ttgctccttt tatggcatac
      961 gcaagagctg gccggggtat atgtcgctgc tgatcatggt atttgctgcg attattgctt
     1021 ccactggaat gccgaatgct gccgatattg agtgcaatgt gtctcctggc tgaacgacat
     1081 attcggatgc tccgctctga gctctcatct gcgcggcagt ccgcggacct ccgggtatgc
     1141 gaagtctctg tccaacatat atgttcgtgc ttgtcaggtt gtttgcagac ataattgcct
     1201 gaactgttgt gccgaatctc cgggcaatca ggaaaagcgt gtcgcctggc tgtacaacgt
     1261 aatctgtgcc gattatgggt ggagctcctg tcggtccagg caagaacaga acttggccta
     1321 ctgagataat gtcggtagtc aagttgtttg tccgccgtat tgcctctact gtcgtgccga
     1381 atctgcgagc gatgagatag agcgtgtcgc cggaccttac aatatatgtt cccggggcag
     1441 gggtcggaaa agggactccc gcgtcaatca cgaccggggt acccaccgat acctgggtaa
     1501 aaagggcttc tacgtccgga gtatacattc tgatgcagcc attggaaacg gcatgtccga
     1561 tggactgtgg ttgatttgtg ccgtgtatgc cgtgcccctg tcgagtgaag cccatccatc
     1621 ttgtgccaaa aaccccgcca gggttgaggg ctttgttcaa tatgctccag ttgcccacgg
     1681 gagttggggt tgctggtttg cctatggcca caggatactg tgccgactcc ctttcgctct
     1741 gcaggagtgt gagtctgcgt tgggataggc tgacatggat tgatgggccc tgccgactct
     1801 ttgagctagc ggaaagggcg agctcagacc cgccgtttgt ggatttgtct ttatctgctg
     1861 aaatacacat tctgcttgcg gaggaccgat cggaatatcc acggtaatcc acagtattgt
     1921 catagcgaag tgagtcatcg ggacagaaca cctcgtccgg aaggggaagg ccgtcaaaga
     1981 ggagtctcca cgtctgggcg tctacgatcc cggtttctgg caggcccaca ctagcctgga
     2041 attgctttac tgcgagttca gttcgtattc cgaacacgtt gtccacttcg cccaagggga
     2101 aacccttgaa ggcgagttgt tcctggagat ttcccacggg tatggctatc atcccgcgca
     2161 taagcacgag cggcttgcgt tcatccaatt tccatcaccg cctattgcct ttctgaggta
     2221 ttatacgcgc tcaggcacaa ataggttgaa ggattcctgc gacgcttgtt gaatatcaga
     2281 agttagcagg tgggctcata tgccaccgat taatataaag ggaggttaca ccatggctgg
     2341 tgtcacatta gagcatgtga ccaagaagtt cggaaacgta gtcgcagtaa acgatgcaga
     2401 cttggagatc aaggacaagg aattcttagt gttggttggg ccttctggat gcggaaaatc
     2461 gaccaccctc cgaatggtgg cgggacttga ggagataacc tcgggcaaca tatacatcgg
     2521 agacacgctg gtcaatgata tccctccaaa agaccgcgat atcgcaatgg tatttcagaa
     2581 ctacgcgctc taccctcaca tggacgtcta caacaacatg gctttcggcc tgaagcttcg
     2641 caagtttcca aaagagcaga ttgacgagag agtcaaagat gccgcgagga tactcggcat
     2701 agagaatctc ctcgatcgca agccgaaggc gctctcagga ggccagaggc agcgtgtggc
     2761 tgtgggccgc gccatcgtcc gcgagcccaa ggtgtttttg atggatgaac cgctatccaa
     2821 cctcgatgcg aagctcaggg tccagatgag gtcagagttg tcaaagctcc ataacaggct
     2881 gcaaacgact atcatctatg tgacgcacga ccagactgag gccatgacca tgggcgaccg
     2941 catagtagtt atgaaggatg gtttcatcaa gcaggtcggt gcgccgctgg agatatatca
     3001 gtatccagac aacatgttcg tcgcaggctt tattggaagc ccggcgatga acttcatccc
     3061 tggcgttctg cgcaaaaagg gtgatggtta catcatggat tgcgagagct tccaaataac
     3121 ggtgccaggg aacaggttca aggatctcgg caagtacatt gacgagagaa tggttcttgg
     3181 aattcgtcct gagaatatcc aggatgcgga tttcgtggaa gatgcgcctg cagacaggac
     3241 ggtcaagtgc atggttgacg tggttgagcc tatgggcgca gaggcctacc tgtatttgac
     3301 ggtgggcccg cattcctttg tggcaagggt caaccctcgg acaacggcgc gagacgggga
     3361 accgcatgca gtcgtgttga atatggaaga gtgccatctc ttcgaagcaa gcacggagaa
     3421 tgccatcgcc attaagtaca catggggata ataatgccgg ggcc
//
LOCUS       DAOSAI010000281         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17429_length_3451_cov_3.586867, whole genome shotgun sequence.
ACCESSION   DAOSAI010000281 DAOSAI010000000
VERSION     DAOSAI010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17429_length_3451_cov_3.586867"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PLM83_08885"
     CDS             <1..602
                     /locus_tag="PLM83_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOK71551.1"
                     /translation="RLSVDQMASAAKQFGLSQPTGLRFTPVEATGAVPDSSVRDFMPG
                     ELASYAIGQQVTVTPLQMAVAYGGIATRGVMYKPHLIRQVIAPDGTVQTQSVPEVMRT
                     AVLSDRTWDYLHHSLSEVTRIGTASSAFRNFGIPVAGKTGTAEVPPGDPHAWFVGWAP
                     ADDPEIVVAVMIERGGGGGASAAPVARKIMEAYFAPTGE"
     gene            683..1363
                     /gene="minC"
                     /locus_tag="PLM83_08890"
     CDS             683..1363
                     /gene="minC"
                     /locus_tag="PLM83_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOK71552.1"
                     /translation="MIFKGTRDGLRLVIDADENIEELKGRLRRRLNEAEGFYSGARVR
                     LDTRGAVVPPEWIEEVRMILEEYGLSLSEEKPGQRRPRQHAAGAGRASAKKVLDDGGP
                     ESGEPIVRGDTILVRRTLRSGQAVSFDGNVVVMGDVNPGSEVLATGDIVVFGSLRGMA
                     HAGSAGNAEACVVALRLIPTQLRIADKITRAPDDETQVPQGPEVAYLREGSIVIEPWT
                     THTGTVLV"
     gene            1363..2166
                     /gene="minD"
                     /locus_tag="PLM83_08895"
     CDS             1363..2166
                     /gene="minD"
                     /locus_tag="PLM83_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOK71553.1"
                     /translation="MSGTAIVVTSGKGGVGKTTAAANIGAGLAMSGPTVALVDADIGL
                     RNLDLVLGLENRIVYDLVNVVEGGCDVQKALIRHKHIENLSLLPAAQSRDKTAVTPEQ
                     MKALTLGLKESFDFVILDCPAGIEQGFKNAIAGADRAIVVTTPEMSAVRDADRIIGML
                     EAEEVKNPWLIVNRIRPDMVKRHDMMNVDDLIDFLKVELLGVVPDDESIIISTNRGIP
                     AVMDEKSRAGRAFLDIASRIMGNDVPIAATLEPDDWFSRFAKWLGISRG"
     gene            2172..2453
                     /gene="minE"
                     /locus_tag="PLM83_08900"
     CDS             2172..2453
                     /gene="minE"
                     /locus_tag="PLM83_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOK71554.1"
                     /translation="MMDFLARLFGRVETETSRECAKNRLRLVLVQDRATLSPRVMEQL
                     KSELIEVISKYLDIDEEDMRVELDTSDSSVALVASIPVKEVKRESVGAV"
     gene            2522..>3451
                     /locus_tag="PLM83_08905"
     CDS             2522..>3451
                     /locus_tag="PLM83_08905"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOK71555.1"
                     /translation="MLIDRRLLSNLDHALLVAVVIAVLFGCAMIYSATRWNTRLTAGN
                     PLLYVKKQLAAAALGAAASLVVLSVDYHYLERFAKWIYLASLLLLASVFVFGKRTKGA
                     IGWIKIGPVAIQPCEYAKIGVVIALAAYLAQQDDLSDLRSFIVPALIVAAPMALVVAQ
                     NDLGSALVLAPVFFAMMYAAGADAKLLLAIVGVILLVIAPFSYFFLLQTHQQMRIKVF
                     FNPGLDPRGYGYNVMQSVIAIGSGGLLGKGYGQSTQARLNFLPEHHTDFIFSVIAEEL
                     GFFGATAALAVLFFIVHRGYSVAQEARDKFGALL"
BASE COUNT          755 a          777 c         1079 g          840 t
ORIGIN      
        1 caagactctc ggtggatcag atggcttcgg ctgcaaagca attcgggctt tcccagccga
       61 ctggcttgcg atttacgcct gtggaggcta ccggagctgt tccagattcc agtgttcgcg
      121 atttcatgcc gggcgaactt gcaagctacg ccattggcca gcaggtgact gttactccgc
      181 tgcagatggc tgtcgcctat ggtggaattg ctactcgagg cgtgatgtac aagcctcatc
      241 ttatcaggca agtgatagcg cctgatggga cagtacagac gcagtctgtt cctgaggtaa
      301 tgcggacggc tgtcctttct gacagaacgt gggactatct tcatcacagc ctcagcgaag
      361 tcacgcgaat aggcacggct agttcggctt ttagaaattt tggcataccc gttgcaggca
      421 agactgggac tgcggaggtt cccccgggag atccacacgc atggtttgtc gggtgggcgc
      481 cagccgatga tccggagata gttgttgctg ttatgataga gcgtggcgga ggtggcgggg
      541 cgtctgcagc gcctgttgcc agaaagatca tggaagccta tttcgccccg acgggcgagt
      601 aggcaggaag gaaatgcatt cctattggag aatcaaacat ggataggctg aatcggcggt
      661 ggttgcggtg aaggctcagg aagtgatttt taagggaaca agggacgggc ttcgcctcgt
      721 tatagacgca gatgagaata tagaagaact caagggcaga cttcgccgaa ggcttaacga
      781 agctgagggt ttttactcag gtgccagggt ccggttggat acgcgcgggg ctgttgtccc
      841 gcctgagtgg atcgaggagg tcaggatgat cttggaggag tacgggttgt ccctgagtga
      901 ggagaagcct gggcaacgaa ggccgcggca acatgccgcc ggcgcgggtc gggcttcggc
      961 taagaaggtt ctcgatgacg gtggcccaga gagcggagaa ccgatcgtgc gcggcgacac
     1021 gatcctcgtc cgaagaactc tcagatcagg gcaggcagtg tcgttcgatg ggaatgtcgt
     1081 cgtgatgggc gatgtcaatc cgggctccga agtcttggca accggcgaca tagtggtttt
     1141 cgggtcgttg cgagggatgg cgcatgcggg ctcggccgga aacgccgaag cctgtgtagt
     1201 tgctctgcgt ttgatcccga ctcagctccg gatagctgac aaaataacga gggcgcccga
     1261 tgacgaaacg caagtgccgc agggtccgga agtagcttat ctgcgcgagg ggtcgatcgt
     1321 gatagaaccc tggacaactc atacggggac ggtgttagtt tgatgagcgg gactgctata
     1381 gtagtgactt ccgggaaagg cggtgttggc aagaccacag cagcggccaa catcggcgct
     1441 ggcctggcga tgtcggggcc cactgtagca ctggtcgatg cggatatcgg gcttcgcaat
     1501 ctcgatctcg tgctagggct cgaaaataga atagtatacg acctagtcaa tgtagttgag
     1561 ggtggttgcg atgttcagaa agcgctaata cgtcataaac atattgaaaa tctctctctt
     1621 ctgccggctg cgcagtcaag agacaaaaca gcagtcacgc ctgaacagat gaaggctctc
     1681 accttggggc ttaaggagag tttcgatttt gtgatattgg attgtccggc aggtatcgag
     1741 caggggttca aaaatgcgat tgctggggct gaccgcgcga tcgtggtaac taccccggag
     1801 atgtctgcgg tgagggacgc agacagaatt atcggaatgt tggaagcgga agaggttaag
     1861 aacccatggc tcattgtcaa caggatccgc cccgatatgg tcaaaagaca cgacatgatg
     1921 aacgttgacg acctcataga tttcctcaag gttgaactct tgggcgttgt tccggatgac
     1981 gaatcgatca taatctctac caacaggggg attccggcgg taatggatga gaagtcccgt
     2041 gctggacgag cttttctcga tattgcatct cggataatgg gcaatgatgt gccgattgcg
     2101 gcgacgctcg aacccgatga ttggttttct cgattcgcca agtggctagg gatatcaagg
     2161 gggtaggcgc catgatggac tttctcgcgc ggctgttcgg aagggtggaa accgagacaa
     2221 gcagggaatg tgctaagaat aggctgaggc ttgtactggt gcaggatcgc gctaccctct
     2281 caccgcgcgt tatggagcag ctcaaaagcg aactgattga agttattagc aagtatttgg
     2341 acatagatga agaggacatg cgcgtcgaac tagacacgtc tgattcatcc gttgccctgg
     2401 tagcgagcat tccagtcaaa gaggttaagc gcgagtcggt tggagcggtc tagcttgact
     2461 ttgcattatg gccccttact ggaccaggtt tgttattcgt gctctttcag gaggccgtag
     2521 tatgctcatt gataggaggc ttcttagcaa tctcgatcat gcattgcttg ttgcagtagt
     2581 cattgcagtt ctatttggtt gtgccatgat atacagcgca acaagatgga atacgcgctt
     2641 gacggcggga aacccgcttc tttatgtgaa gaagcaattg gctgctgcgg cgttgggggc
     2701 tgctgcgtca ttggtcgtcc tcagtgtgga ctatcactac ttggaacggt ttgccaagtg
     2761 gatctacctg gctagtctat tgctgcttgc aagtgttttt gtttttggga agcggactaa
     2821 gggtgcgata ggttggatta agataggacc cgtggccata cagccatgtg aatacgccaa
     2881 gataggggtg gttatagccc ttgctgcgta ccttgctcag caggatgatc tttccgattt
     2941 gcggagtttc attgtgccag ctctgatcgt ggcggcgccg atggcgcttg tggttgcgca
     3001 aaacgacttg ggttctgcac tcgttcttgc ccccgtgttt ttcgcaatga tgtatgcggc
     3061 tggagctgat gcgaaactgt tgttggcgat tgtcggggtt attcttctgg tgatagctcc
     3121 gttttcctat ttttttctgc tgcaaactca tcaacagatg aggatcaagg tattcttcaa
     3181 tcctgggctt gatccgcgcg gatatggata caatgtgatg cagtcagtga tagcgatcgg
     3241 atcgggtggg ctattaggaa aggggtatgg ccagagcact caggctcggc tcaatttcct
     3301 gccggaacac cacaccgact ttatattctc cgtgatcgcc gaggaattag ggtttttcgg
     3361 ggcgactgcg gcgctggcag tcctcttttt catagtgcac agaggttata gcgtggccca
     3421 ggaggcgcga gacaagttcg gcgctcttct g
//
LOCUS       DAOSAI010000282         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17596_length_3425_cov_3.564688, whole genome shotgun sequence.
ACCESSION   DAOSAI010000282 DAOSAI010000000
VERSION     DAOSAI010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17596_length_3425_cov_3.564688"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1077)
                     /locus_tag="PLM83_08910"
     CDS             complement(130..1077)
                     /locus_tag="PLM83_08910"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOK71556.1"
                     /translation="MAFDKRLLYEIAKNYYELDKTQSEIASSLGISRSQVSRALKQAK
                     DEGIVVVKLIPPGLDFADLEEKLRSTFGLSDAVVISGKAGPSRLLTQNLGMAGANYLD
                     RELRPGQVIGVSWGATLRELVAALGSIKPEPREIMAIPLLGGQGQASPDLQVNDIASR
                     VAMAFGGSHLYLHAPSFVDTPEARNMMMNDSNIRNVASKWAAADVAVVGVGCFVPPST
                     LLEEGGFPPDELEDLVRMGVVGDICMRFFDEQGQPVDTPLGDRIIGASLDQLRAIDCV
                     VAVAGGVNKAASILGALRTGVVDVLITDDLTARRVLEIS"
     gene            complement(1841..2431)
                     /locus_tag="PLM83_08915"
     CDS             complement(1841..2431)
                     /locus_tag="PLM83_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK71557.1"
                     /translation="MTQCYGMGTRGIICRTAVRVFAEKGYDAASMDQIALEAGVAKGT
                     VYYHFKSKSNLFSAIIENGLDALIEDVEGATLRSTDPVEQLSAIFDALIDYAQTFDDF
                     MYLLFKEALAPGREWASDVVVYWHRFVNIIEDVVARGKSMGRIDNVDTDAITQSIVSS
                     LATAALGWATGIGDEMWAERVLHGVKGITLRGIVVA"
     gene            complement(2438..>3425)
                     /locus_tag="PLM83_08920"
     CDS             complement(2438..>3425)
                     /locus_tag="PLM83_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71558.1"
                     /translation="ESAKPILVKLSDTISEAMSSLPMDQLGTAAELAPKLLEQATELN
                     DKAQRVSKGLSSCSQTITQAKSASSESMAALEALAKSNPEITKSLQYKKLESSIKKQD
                     ALLETAKSGGRVGFSQVPSIDELAEDAKSVASVGSKAKIAISLASNQLSKVSLDSVDD
                     YLNQAMGAVEVLNALLYGGTVQGHEVPSIDEICDNLRKLDEGAHDLKTGLSLLAVGGT
                     VDGQNVPGLDTAVSGLEALVDGISKLSNGTVELTAGTKELASGLGRIRNEGTSEMKKG
                     INEGLAEALKAKAQLDAMKVRLDQYDTFEGKPAGAAGEVRFLMKVKAPSNKG"
BASE COUNT          758 a         1009 c          795 g          863 t
ORIGIN      
        1 agcggcattt ggattgcctc cttaaataga tttatctgaa cccacgctcc cgcgcgagga
       61 accacccgtc ataggccagc cgttgttgtc acgccgccaa cttgaatctg ccacgatcca
      121 attgagtaat tacgatattt caagcaccct tcgggccgtt aggtcgtcag taatcaacac
      181 atccactact ccggtcctta gcgccccaag tatggatgca gctttgttga caccacccgc
      241 gacggccacc acgcagtcta tggctcgcag ctgatccaga ctggctccaa tgatgcgatc
      301 ccctaaggga gtatctacag gctgcccctg ttcatcaaag aacctcatgc atatgtctcc
      361 aactacgccc atcctaacaa gatcttctaa ttcatcagga ggaaagccgc cttcttcgag
      421 aagggtggaa ggaggcacaa agcaacctac tcctaccact gccacgtctg ccgcggccca
      481 cttgctagcg acgttcctta tgttgctgtc attcatcatc atgtttcggg cctcaggtgt
      541 atcgacaaat gaaggtgcat ggagatatag gtgggatccg ccaaatgcca tggctacgcg
      601 ggaagcaata tcgtttactt gaagatcggg gctggcctga ccctgtccgc caaggagagg
      661 aatcgccatt atctcccttg gttccggctt gatactgcct aatgctgcta cgagttcacg
      721 taacgtcgcg ccccatgaaa ccccaatcac ctgaccgggc cttaactccc tgtccaaata
      781 gttcgcgcct gccatcccca agttctgcgt caacagcctt gaggggccag ccttgcctga
      841 gatgaccacc gcgtcactta agccaaaggt agaccgtagt ttttcttcca aatcggcaaa
      901 gtcgagtcct ggaggaatga gcttgacgac gactattccc tcatctttcg cctgtttcaa
      961 ggccctggac acttgacttc gcgatatgcc taagctggag gcaatctcac tctgtgtctt
     1021 atcgagttca tagtagttct tagcaatttc gtacaacaga cgcttgtcaa aagccattgt
     1081 aataccccca agggtctttt taggctaggg aacgctctca tcagacactc ttttgcacat
     1141 ttgttcagca cgtttgtgct gatgggacta acattctacg aagaactcca tattcctgct
     1201 tggagaatga ttttttatgc caggtctcct cttctgtccc aaaagttacg tgagtctcga
     1261 tgagttcccg cgcgattttc gagtatgcat ttggaagggc gacttgggcc tgccttcaag
     1321 gaatatgtct attgtttagt cgcttttcgg gagcgggtgt ctaactttta gactggcccc
     1381 gtctaaattt tagccacttg aagctctaag gcaatcttgg gcagggataa tccggcatgc
     1441 cttgccataa ggacccagtt tccggagata aattactatg tggaagtgca tgtacccata
     1501 tattggattg gggcgcagac aggtgtctga gttttagacc gctaccgtct attctttgaa
     1561 cgctttgcga tcgcaaatgg ctagaattta gacaaacctg ctcgcccgca cggcgcttcc
     1621 ctgggcttct accgaaggct aaacagaccc cggctaagtc tgtctaccta actacgaggc
     1681 cgtcctaacc gggttgtctt cccgctcatc aggcaggcag ctcaacagac ataccgggca
     1741 aacacgcaaa catccccgga gccgctgccc cggggatgtt ctatcaacat ctttccgttg
     1801 ttcaactaca gccaattgcg ctgtctacaa cccgtgtatg ctacgcaacg acaatgccac
     1861 gcagcgttat gcccttcact ccatggagaa cacgttctgc ccacatctcg tctccgattc
     1921 ctgtggccca tcccaaggcc gctgtggcaa gagatgacac aatcgattgt gttatagcat
     1981 cagtgtctac gttgtcgata cggcccatag atttacccct tgctactacg tcttctatga
     2041 tgttgacgaa cctgtgccaa taaacgacca cgtcacttgc ccactctcgc cccggggcca
     2101 atgcttcctt aaacagcagg tacatgaagt cgtcgaaagt ctgggcatag tctatcagcg
     2161 cgtcaaatat ggcgctgagc tgctccacag gatcagtgct gcgcaaggtt gctccctcaa
     2221 cgtcctctat taaggcatcg agtccgtttt cgatgatagc ggaaaacaga ttgctcttgc
     2281 tcttgaaatg gtagtagaca gtgcccttag ccacccctgc ctccaaggct atctgatcca
     2341 tggatgcagc atcatatccc ttttcggcga atacacgcac tgccgtgcgg catatgatgc
     2401 cccgtgttcc cattccgtag cattgtgtca caacaaccta ccccttgttc gagggggcct
     2461 tcaccttcat cagaaagcga acctcgccgg cggcacctgc gggcttccct tcgaacgtgt
     2521 catattgatc gaggcgcact ttcatagcat ccaattgagc cttagccttc agcgcctcag
     2581 cgagcccttc gtttatgccc ttcttcatct ccgacgtgcc ctcatttcgt atcctgccca
     2641 acccggaggc aagctccttg gtgccagcag tcagctcaac ggttccgttg ctcaacttgc
     2701 tgataccatc gacgagagcc tcgagaccgg atactgcagt atcaagcccg ggcacattct
     2761 gaccgtcgac tgtaccgccc acagcaagaa ggctcaaccc agtcttgaga tcatgcgctc
     2821 cttcatcgag tttgcgcaag ttatcgcata tctcatctat gctaggcacc tcgtggcctt
     2881 gcactgtgcc tccatatagg agggcattga gaacctctac ggcccccatg gcctggttga
     2941 ggtaatcatc cacggaatcc agcgacacct ttgatagctg gttcgatgcc agcgaaatgg
     3001 cgatcttagc cttgcttccc acggaggcca cactctttgc gtcctccgca agctcatcga
     3061 tgctcggcac ctgtgagaat ccaaccctgc ctcccgattt ggcggtctca agaagggcgt
     3121 cctgcttctt gatggaagac tccagtttct tgtactgcag cgatttcgtt atctccggat
     3181 tcgacttggc aagcgcctca agcgcagcca tagactccga actagccgac tttgcctggg
     3241 ttatcgtctg cgagcaactg gacaacccct tggataccct ttgagccttg tcattgagtt
     3301 cggtcgcctg ttccaaaagc ttaggcgcca attccgccgc tgtgccaagt tgatccattg
     3361 gcagcgacga catcgcctcg gatatcgtat cggaaagttt gaccaggatc ggcttggctg
     3421 actcg
//
LOCUS       DAOSAI010000283         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17666_length_3415_cov_3.202976, whole genome shotgun sequence.
ACCESSION   DAOSAI010000283 DAOSAI010000000
VERSION     DAOSAI010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17666_length_3415_cov_3.202976"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1534..2052
                     /locus_tag="PLM83_08925"
     CDS             1534..2052
                     /locus_tag="PLM83_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017533230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOK71559.1"
                     /translation="MSSSGGRSVRGEVGPLEFEPSGPFENSGLHCRETHAAALRSPVL
                     MRGRLPAFADAAGMFLLCVYPLARGEEFGAEPSDEGIDIALAEYDVELSSIEEREFPR
                     LTPTETEVMNLLAQGKTNRAIAEEMFISINTVKTHIRSIYQKLGVHTRGSAVRLAVAA
                     GLLDSWSPPPSP"
     gene            2497..>3415
                     /locus_tag="PLM83_08930"
     CDS             2497..>3415
                     /locus_tag="PLM83_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK71560.1"
                     /translation="MIHADEACLILNPTLGTWVKVSRYAGVMCERFLGVDIQGITDSV
                     GPAYGKETLDGLLGLMRDLEARGLLVYGPWNGGGKADVAAAEKRAAQVCAAPARIYVS
                     VTDRCNLYCETCYRHAFENDAPTESVLWTIREAGMFRPAELMITGGEPTLRADLPVLL
                     EEATFNAPKVILATNATLITQEMAEAIASLGVCVQVSIDSGDEAAHDMIRGEGSFKAA
                     IRGIERLVRAGTEHVELVSTIVDPATFNPDDMIELASKHGATFHASLFQEVGRGACSR
                     LKGRRKCPKDLAEAMLRYLLRACHRNALPE"
BASE COUNT          777 a          773 c         1028 g          837 t
ORIGIN      
        1 tcctttgaac gatcatgcca gaacgcggtc tgtgtcgata gaacgaagct acaccccggt
       61 gaggccttcg cgcctagact aggcatgaag ggtttgcctc gggtgggtat ctcaggccgt
      121 ggtgcgcgtc agtctaggat gctgtgggaa gacgaaggga tatgaagaaa agcagggggt
      181 tgatgggaac tgactttgca agggtttccc atccaactgt ccttgccgga atacgagtag
      241 gcaggggatg tttccctgtc ttttttaatc aatgttaagg gtgttgctac aagaaccaga
      301 gatgctcggc caagctactt catcggatag tctgaaaggc ctttagcacc cgttgtatgc
      361 cgacatctat caccgcctgt cctgcgtggt ttttgactgt gctctgtaat gcctgaaagc
      421 gcctatatgg tgacaggaag cacagtgtaa atgcgatttg caagagaatt tggcaagtgc
      481 gaggtttcaa tgttttgggc catgcgcaga agaagtatca ctgagtgccc agatggtttt
      541 gatttcgagc ctgggctggt gcattaccaa gatagcgcgg atgttcagtg tctatgaaaa
      601 catggcgtcg aaatcagttt agccatgatg tataacctac tgaaatctct tggatatcgg
      661 catagtcgcc caccgcatgc gtcaaaacga gtatatgatc tgcaggcgat ggccaaaatg
      721 gacgcaatca cggaggccct gagttcacca gcggctggaa atcacgtgct cttccaggat
      781 gaatgcgacc tctatcttct gcagccacag tattcgctga caggtttttg aagttttggc
      841 ctgccaatga gaagtaattc cacgtaggaa ggacgaggac ggcttcaagc tagcctgttt
      901 atagggtgct ccccgaccct acgcagtagc ggggtttcca cgtaacctgt gtaagtagca
      961 ggtttgtgac gccgtggggg agatatggcg gcttttaggc gaagctcatc tgaagggctg
     1021 gatggacttc tgttgacttt cgcgcgatac atgtctggtc ttcgaaaccc gctggggttc
     1081 atgtgccgaa accagatgcc gagaaccgtg taaaagacat gatcacttac tacgcattcc
     1141 cttgtgcctg gctcagaggt cggttatgat ctcttggcca ggctgttatt cctgtcggca
     1201 cttaatcagg tggtccaccc gccggggcga tgtagccgga gctacaaggc ggagcttctg
     1261 cacgccaatg gacggggacg agtagcggag gcctcgaaat cgttggactg gacgtctatc
     1321 ctaatcagta tgaggctttg cacaagataa tacggtcgac tgaggaatac atcgaagtcc
     1381 atcgaaagca ggtaacagag cctagattag ggaatgtttg actggtaacg catgggatgc
     1441 tatactcttt atggtgattg ttgtcataag gggcacgatt cttcttggat tgctttctag
     1501 gcgacggaaa ttcataagga ggggggattt tccatgtcga gctcaggggg acgttcggtt
     1561 cgtggtgaag ttgggccttt ggagtttgaa ccgtctggac catttgagaa ttcgggattg
     1621 cactgcagag agactcatgc cgccgccttg cgcagcccag tattgatgcg gggcaggctt
     1681 cctgcgtttg cagatgcggc agggatgttc ctgctgtgcg tatatcctct agcgaggggg
     1741 gaggaatttg gggctgagcc atccgacgaa ggaattgata ttgctttagc agagtatgat
     1801 gtcgagcttt cgtccattga ggagcgagaa tttccgaggt taacccctac cgaaactgaa
     1861 gtgatgaacc ttcttgctca gggcaagacc aatagggcaa tagctgagga gatgttcatc
     1921 tcgataaata ccgtcaaaac tcacattcgt tcgatatatc aaaaactggg agtgcacacc
     1981 cgggggtcgg cagtcaggct ggcggttgct gccggattgt tggacagctg gtcacctcct
     2041 cctagcccgt aaaatcatcc tttcgggcaa tacccttaag ctccctgcga tggtactatg
     2101 tgggtgcaag cggcgccgtg gcgtggtcgc catctacccg attgtagcat cgagggaggt
     2161 gaaatggatg tttcgcattc aggcaggtcg taagcatagg ttgaatgtct ctgtcaacag
     2221 cttctgcttt tcgtttccgc tttggggcgt gatgctcacc attggatgat cttcgtttcc
     2281 ccagactact agtcaccatc atccattagc gggcgtagct ccaactgtgc cagcgggggc
     2341 caatcacaag agattggttc ccgcgcctat gtgcgcgcgc tgtggctttg aagggaggag
     2401 tagggcggcg atgtggcccg atgttggcgg ggcgcaaccg ccttttcgct gatgaactcc
     2461 gaattgttgg gatactctct agcgccggat gcacggatga ttcatgcaga tgaagcttgt
     2521 ctcattctca atccgacgct cggaacttgg gtgaaggtca gccgttatgc aggcgtgatg
     2581 tgcgagaggt tcctcggagt cgatattcaa ggaataactg attctgtagg gccggcttac
     2641 ggaaaggaga cgctagatgg cttattgggg ttgatgcgcg acttggaagc ccgaggattg
     2701 ctcgtctacg ggccctggaa tggaggcgga aaagcggacg tagcggcggc agaaaaaagg
     2761 gccgctcagg tttgtgccgc gcctgctagg atatatgttt ccgttacgga caggtgcaat
     2821 ctatactgtg agacatgcta cagacatgct ttcgaaaacg acgccccaac tgagtcagtg
     2881 ctttggacaa tacgagaggc agggatgttt cgtccggcgg agctgatgat cactgggggt
     2941 gaacctactc tcagggcaga tcttcctgta ttgctcgagg aggcaacatt caatgctccc
     3001 aaggtgattt tagctaccaa tgctacactg ataacgcagg aaatggctga agcaatagcc
     3061 tctctggggg tctgtgtcca ggtgagcata gattcagggg atgaggctgc acacgatatg
     3121 atcaggggcg aaggaagctt caaggcggcg attaggggca tcgagaggct cgtgcgtgcc
     3181 ggtacagagc acgttgagct tgtgtcaacc attgttgacc cagcgacatt caatccagac
     3241 gacatgattg agcttgcatc gaagcatgga gcgacattcc atgccagcct ttttcaggag
     3301 gttggtcgag gagcatgttc acggctaaaa ggacgcagga agtgtccgaa ggatcttgcg
     3361 gaggcgatgt tgcgctatct tctgagggcg tgccacagga atgctctgcc ggagg
//
LOCUS       DAOSAI010000284         3409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17714_length_3409_cov_3.121348, whole genome shotgun sequence.
ACCESSION   DAOSAI010000284 DAOSAI010000000
VERSION     DAOSAI010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17714_length_3409_cov_3.121348"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..1402)
                     /locus_tag="PLM83_08935"
     CDS             complement(302..1402)
                     /locus_tag="PLM83_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK71561.1"
                     /translation="MDRGSSVPLYIQLKELLRDKIESGDLGDGELLPTEQELCEQFNL
                     SRYTVRQALGELVKEGYIERVRGLGSFVRRVPKVANAPAVGIIVPYTDDFYTSSIIRG
                     VEGVARSEGHNLIFANSNNHPEQEETLLRKLRNDGVRGLIIFPCEDSVYSVAVEGLMS
                     IGFPVVLVDREYKTLDCPAVVSDNVDGGEKAVDYLVSLGHKRIAFAMTIFNHTSSVQD
                     RLLGYRTALARHHIPWDKSLLLTYAPRLYDAPEDDADHIAPLRRYLAREDRPTAIFAV
                     NDLVALDILRAAAEVGLDVPGDLSVVGFDDIPVVSELPTPLTTVAQPHLEIGAVGAKM
                     LFHIIAGRTLERTRVVLPVKLVVRQSCSELSQ"
     gene            1759..3024
                     /locus_tag="PLM83_08940"
     CDS             1759..3024
                     /locus_tag="PLM83_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOK71562.1"
                     /translation="MKNRRRFLVTVSLLIACSILAVQPLATIAAGFETFKLPIAEFEP
                     GMPSLYLRGPVEPAKNVILLISDGTGINHRTLVRIASGGANSKLPSERLFEVGLANTH
                     SLDSLVTDSAASATQIATGNWVPNRTISVNADGEPLQTILELAKTLGKSTGIITTDRV
                     TGATPAGFAGHVPDRDMKNEIAQIILENDSVDVLIGGGKKNFQSPVAGKKGDRTDGRD
                     LLEEAAERGVTICNSIYELRADDSPRTWALLSSGSMTAAVKRNYTLGDLTSEALRRLS
                     QNPEGFFLMVEGDQIDGGGHDNDIWRMALELLDFETAVEVATEFAENRDDTLVIVVSD
                     HETGGLAITNGQAQSTEVEYGWIHGGHTGTPVAIYSSGPNSFLLGGHIYLADISKIIA
                     FGWDIYDFGPDRAVEGPGAKWLLSKQNQQ"
BASE COUNT          896 a          903 c          837 g          773 t
ORIGIN      
        1 agatatgcga ccacagcatc tccagcgccg gaacactaac agcccaaccg accatatgtc
       61 cggacaatta tgggcgcaaa agggctacag tgtctcggac gccacctgca gatagtcttt
      121 aatggccagc ataatagggt gtgtctcatg attagaccat ccaagagtgc taatcgggct
      181 acaccccata ctgccagcag ttgttgtttg tacgtacatc gtcacaccct attctacatc
      241 gcctggcgta atcctgcttc atgcacgaaa tcgcacatct tcctaagata gatgctatgc
      301 gttactgact cagctcactg catgattggc gcacaacaag cttcacgggc aagaccactc
      361 ttgttcgctc gagggtgcgg ccagcaatta tgtggaaaag catcttggcg ccaacagcgc
      421 caatttccag atgcggttgg gcgacggttg tgagcggggt gggcagctcg gatacgaccg
      481 gaatatcatc gaacccgacc accgaaagat cgcccggaac atcgaggccc acttcagcag
      541 cagcgcgcag aatgtccagc gctacaaggt cgttgactgc aaaaatggct gtaggtctgt
      601 cctctcttgc aaggtatcta cgcaaagggg caatatggtc ggcatcgtct tcaggcgcat
      661 catatagcct cggcgcgtac gtcaacagca acgatttgtc ccacggtatg tggtgccggg
      721 ccaaggccgt tctgtaacct aagagtcggt cctgtactga actggtgtga ttaaatattg
      781 tcatggcaaa agcgatcctt ttgtggccca atgataccag gtaatccacc gccttttcgc
      841 cgccatcaac gttatccgac acaacagcgg gacaatccag agtcttgtat tccctgtcaa
      901 ccagaaccac gggaaatccg atagacatca gaccttctac agcaacacta tacacgctgt
      961 cttcgcaggg gaaaatgatc aacccccgca ccccgtcatt ccgcaatttc cgtaggagtg
     1021 tctcttcttg ttcaggatgg ttgtttgaat tagcaaagat gaggttatgc ccctcagatc
     1081 tggcaacccc ttcaacccct cttatgatgc tggatgtgta aaagtcatcg gtgtaaggta
     1141 caataatccc taccgccggc gcatttgcca ctttgggaac acgcctcaca aatgacccaa
     1201 gtcctctaac cctttcaatg taaccttcct ttacaagctc tcccaaagcc tgtctcacag
     1261 tgtatctgct caggttgaat tgctcgcaca gctcctgctc tgtgggcagc agctcaccat
     1321 cgcctaagtc tcccgattct atcttgtcgc gcagaagctc ctttagctgg atgtaaagcg
     1381 gaaccgaact gcctctatcc attatttgca ttgctccctc acacggattg tacggacata
     1441 atgtgtttat catattctac tcattacggc tgtatcctgc aaacgagcac catttaggat
     1501 tcgagctaga ctcgtatcac ggcctttgga ccggcaacgc caaagtcgct caaagcttgc
     1561 gattatgcgt tacgttcaca aggaggattc agaacactgg cggcgaatca atgagtcgca
     1621 gcacatgtac ggacaaatag gggaggccaa tcgcccttgt ttaagccata atggcccgaa
     1681 gctttcgaat tcctcgccag cacttgctgg ttctgcaggc attcgcaagg aaaactctat
     1741 tctcgggaga tgatcatttt gaagaatcga agacgatttc tggtcacagt aagtctactg
     1801 atagcatgct caatcttggc cgttcaacct ctggctacta tagcagcagg gtttgaaacc
     1861 ttcaaacttc ccatcgcaga attcgagcct ggcatgccaa gtctttacct gcgtggccct
     1921 gtcgaacctg ccaagaatgt aatactgctc atctcagacg gtacaggaat caatcatcgc
     1981 actctagttc ggatagcgtc cggtggcgca aattccaagc tgcccagtga gaggttgttc
     2041 gaagtaggcc ttgcaaacac tcattcactc gactctcttg taactgattc tgcggcatca
     2101 gccacccaga ttgccacagg caactgggtt cctaacagga ctatcagtgt aaacgctgat
     2161 ggcgagccac tgcagaccat tctcgaactc gcaaaaactc taggaaaatc caccggcata
     2221 atcacaacag atagagttac gggagcaaca ccagccggat ttgctggcca cgttcctgat
     2281 agggatatga aaaacgagat cgcccagatc atactggaaa acgatagcgt ggatgtgttg
     2341 ataggcggag gaaagaagaa ttttcagtcc ccagttgcag gcaagaaggg cgatcggaca
     2401 gatggccggg atctgctcga ggaagcggca gaaagaggag taactatatg caacagcatt
     2461 tacgagctca gagcggatga ctctccgcgg acatgggcct tgctcagcag cggatcaatg
     2521 acggcggctg taaagcgaaa ctacaccctc ggcgatctta catccgaagc cttaagacgc
     2581 ctgtcccaaa atcccgaggg tttcttcctg atggtagagg gagaccagat agacggcggt
     2641 ggacacgaca atgatatatg gcgaatggct ctggaactct tggattttga aacagctgtt
     2701 gaggttgcca cggagtttgc cgagaacaga gacgacacat tggtgatcgt agtgtcggat
     2761 catgaaacag gaggcctagc tatcaccaat ggtcaagcgc aaagcactga ggtggaatat
     2821 ggctggattc atgggggcca tacaggaact cctgtggcca tttacagctc tggacccaac
     2881 tcgtttctgc tcgggggaca catatacctt gccgacatat caaagataat cgcttttgga
     2941 tgggatatat acgatttcgg cccggatagg gctgtggagg gaccaggggc caaatggctt
     3001 ctgtcgaagc agaatcagca gtaaatccct agaccatgga taaatgcaat ctccagctgc
     3061 ggggagggca aatccctccc cgcttttctt gttctccagt ccccacatca tacttcgccg
     3121 cgtaccagtc ttctcgactc aacagcttag tgtggatatg agcgccagat tctgcagaac
     3181 caaaatccgg ccggaaataa acacgccctg aattaaaaac catagcacaa gcaaggttgt
     3241 tggttcggac gaagacaaca cacaagcctg tcagcagtgc taacccgaaa aggctgtgcg
     3301 aatttccgag aatgtgaaag gcaaaaaacc gagctgagag tgcagccgag catgacatcg
     3361 agtagggtgg cgccaggtaa tcagcctggc gtcatccctc tcacagaac
//
LOCUS       DAOSAI010000285         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17746_length_3405_cov_3.074925, whole genome shotgun sequence.
ACCESSION   DAOSAI010000285 DAOSAI010000000
VERSION     DAOSAI010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17746_length_3405_cov_3.074925"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLM83_08945"
     CDS             complement(<1..469)
                     /locus_tag="PLM83_08945"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HOK71563.1"
                     /translation="MRIDTSGKRKTILLALIFVAAVAAGMCLGARGAVVAALFDNERI
                     CRGVSIDGVSVGDLTVSEARSRLEELVREYEESPISMRCQDAIWRVNPGDLGVRIEFE
                     AALRAAHSLGRSGHFCERIAERASISRSGYDISWTVTVDEQLLRDFVFAMAQQV"
     gene            1721..2344
                     /gene="yyaC"
                     /locus_tag="PLM83_08950"
     CDS             1721..2344
                     /gene="yyaC"
                     /locus_tag="PLM83_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HOK71564.1"
                     /translation="MDSSSDAMAETCQPALAHREKTPFRAHVDTPDVSSSLAQALLAN
                     LGAPPGPNAPSLIIVCVGTDRSTGDALGPLVGSNLAERQIIHESVKVIGTLENPVHAG
                     NLSDIIVQIRPAQPGRCRLLAVDACLGKLGSVGYITVAEGPLKPGTGVNKDLPPIGDA
                     HITGIVNVGGFMEYLVLQNTRLSLVIRMAEAIAGGIVNYLTSEDQKT"
     gene            complement(2345..2992)
                     /locus_tag="PLM83_08955"
     CDS             complement(2345..2992)
                     /locus_tag="PLM83_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOK71565.1"
                     /translation="MQFRDRAEAGALLGRRLQQETWDDPIVLALPRGGVPVAECVADA
                     IGGELGVVVTRKICAPHNRELAIGAVDADGRMFIDSRIVQALAVDDSYIRAETSRQLQ
                     EIERRMAEYCENGDVPNLEARDVVLVDDGIATGYTILAAARYVRRHGPARTVIATPVC
                     PPDVFSKLQEEADMVVSLLLPAHFIAVGQFYEDFSQVSDADVRNILTRVRTEGSR"
     gene            complement(3098..>3405)
                     /locus_tag="PLM83_08960"
     CDS             complement(3098..>3405)
                     /locus_tag="PLM83_08960"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOK71566.1"
                     /translation="GFVKGTLRVDGDLIIGADGVVVASVTAANVTVAGELRGEVNSPG
                     RLDIAPTGQMLGDVIVGSLNVDDGALFLGQCNMKDEVRSQPKVLAAPDDSGNSDSQQ"
BASE COUNT          821 a         1027 c          823 g          734 t
ORIGIN      
        1 caacctgctg cgccatggca aagacaaagt ctcggagcag ctgctcatcg actgtcacag
       61 tccagctaat gtcgtaaccc gaccgagata ttgacgctct ctcagcgatc ctttcgcaga
      121 agtgaccaga ccgccccaag ctatgagcag ccctcagggc ggcttcgaac tcgatcctaa
      181 ccccaagatc gccagggtta acacgccaga tagcgtcttg gcacctcatt gagatcggag
      241 actcctcata ctcccgaacg agttcttcca ggcgcgaacg agcctcggag acagtaagat
      301 ctccgacact gacgccgtca atcgacaccc cccggcatat gcgctcattg tcgaagagcg
      361 ctgccactac ggcgcccctc gcacccaggc acatgcctgc agccacagcc gcgacaaaga
      421 tgagcgccag gagtattgtc ttccgcttcc cactagtatc aatacgcata agaaatctct
      481 tgcctccgca atatgaatag ggtaaggttg tgaccaatat taccacaaaa gtgaacaccc
      541 ttgcacgcca tgtggacccg ggcacaaacc atcgcgccag gcctgccctg aaaggaggcc
      601 cgcctccggc caggtggcgc ttttgaggcc aaaatggtta cgagatttca acagttaagg
      661 ccatgcggca cacatccaca gaccgagtat cacagagagc ccagatagct ttaatctcaa
      721 gccaaggtgc tgtatcgcca ggatagagca ggtattcaat gcctctgcga cacacctcgc
      781 ggtggaatgg ctctgcaggt ttcactggtt ctatataaca ggatgccaaa aaggatcctg
      841 cagaatttgt gcatctcttt agcatctgga gcgttgggaa gctacgttct catgtcatat
      901 caaaagacgg catcgacgtc gtttggccac gatttacgcc acaatagggg ccccgagttc
      961 catagtgtct ggaaacctcg ttctcgtccg ggatgagata cgaccttggt cttctggcgg
     1021 ctatgagggc tatgcagtaa tcaaacccgg attccttcgg tttgaatatc cgcaaaggcg
     1081 gctgcgtcca ccatcaatcc gatagagtgg gttagcttga gttccttgaa tacgtcgttt
     1141 cctactaccc gacaggcgga ttcacattat actggtcaac tacagcatcc acaaaggtaa
     1201 agccgcattt catagtttct aaccccaaag agaggataag gcggctttcg agagctgcag
     1261 ttagagccaa cagacgctat ggcttgatga atgcgcttat caccgcggtg attgcttttt
     1321 ggcagcctaa cttggtccaa gattcaaact ctagccgcaa gaacaatgcg tgcccctcag
     1381 ctcacgcctg acagtagcac cggctcttta ctagtttccc gctattggcc ggccgtgtac
     1441 agccatcccc cagttccgcg ctatagatag atgcagtccg ggaaaagcct caaggtgcca
     1501 atttcccctt acgcgaatga ttctccttct gttgccagac gctttttcgc tccaacttct
     1561 gcgtaccatc ctgtaggcga ttgcatcgtg gacaagcgta ccccgcagac cacatataag
     1621 gggcagcaca ctcatatact tcaaatgccg caacctagag aatcattccc gtccattgct
     1681 gcgaaacggc gctcagacaa gggaggtgtg gggtcgcaaa atggatagca gctctgacgc
     1741 catggccgaa acctgtcaac ctgcactggc gcaccgagag aagacaccct tcagagccca
     1801 cgtcgacacg cctgatgtga gcagttccct tgcgcaggct cttctggcaa atctgggggc
     1861 tcctccaggt ccgaacgcac ccagccttat catcgtatgc gtcggtactg acagatcaac
     1921 aggggatgcg ctgggaccct tagtcggttc caatcttgct gagcgtcaga taatccacga
     1981 atccgtcaaa gtaataggca ccttggagaa ccctgtccat gcaggcaacc tctccgacat
     2041 cattgtccaa atcaggcccg cacagccggg cagatgcaga cttcttgcgg tcgatgcttg
     2101 cctcggcaag cttggaagtg tcggctacat caccgttgcc gaaggaccgc tcaagcctgg
     2161 aaccggggta aacaaggacc ttcccccaat aggcgatgcg cacattacag gaatagtcaa
     2221 cgttggcggg tttatggagt atctggtgct tcagaacacc cgtctgtccc tggtgatacg
     2281 gatggctgag gcgatagcgg gcgggatcgt aaactactta accagcgagg accagaaaac
     2341 atgatcagcg actaccttct gtgcggacgc gagtcaagat gttgcgcaca tctgcatccg
     2401 atacctgcga gaagtcttca tagaattgcc ccacggctat gaaatgcgcc gggagcagca
     2461 agctcactac catgtccgcc tcttcctgga gcttgctaaa aacatcgggc gggcagactg
     2521 gcgtggcaat cactgtacgc gctggaccgt ggcgccgcac gtaccttgcg gcagccagaa
     2581 tcgtgtatcc cgtggcaatg ccgtcatcaa caagcaccac atcgcgggcc tcaagatttg
     2641 gcacgtctcc attctcgcag tattctgcca tcctccgctc aatctcctgc aattgccgtg
     2701 atgtttcagc gcggatgtag gagtcatcta cagccagtgc ctgcacaatg cgtgaatcta
     2761 taaacatcct cccgtcagca tctacagcgc ctatggccaa ctcacggtta tgtggagcgc
     2821 agatctttct cgttacaact actcccaact ctccaccgat ggcatcagct acacactctg
     2881 caacgggaac gcctccgcgc ggcaaggcca ggacaattgg gtcatcccat gtttcttgct
     2941 gcaaccgtct ccccaaaaga gccccagctt cggctctgtc acgaaactgc actgacaacc
     3001 ctcccattcg attcaatttg ctgcatatca aaagagggca agcaccttcc aaaggattgc
     3061 ttacccccaa cgatcccaca ttatttcctc aagccggcta ctgctgagaa tcggagtttc
     3121 cgctgtcatc tggagctgcc aggaccttcg gttgggaccg aacctcgtcc ttcatgttgc
     3181 actgcccaag aaacaaagcg ccatcatcca cattcaagga tccgacgatg acatcaccca
     3241 acatctggcc agtgggtgct atgtccaatc ttccgggact attcacttca cccctgagtt
     3301 caccggctac ggtaacattg gccgcggtta cagacgccac cactacgccg tcggccccaa
     3361 ttatgaggtc gccatccacg cgcagtgtgc cctttacaaa gccat
//
LOCUS       DAOSAI010000286         3395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17813_length_3395_cov_3.288323, whole genome shotgun sequence.
ACCESSION   DAOSAI010000286 DAOSAI010000000
VERSION     DAOSAI010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17813_length_3395_cov_3.288323"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PLM83_08965"
     CDS             complement(<1..1204)
                     /locus_tag="PLM83_08965"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOK71567.1"
                     /translation="MSVVSDNGDTRVVSGNAGTDSGMSKQGTSADGKMRIETDSLGKR
                     QVPVDAYYGIQTLRGQENFQITGLSVHPALIRGLIMVKKATALAHSKLGQIPSRIGQA
                     IAEAADDALAGKLDSSFMLDPIQGGAGTSINMNVNEVLANRAIEILGGVKGDYSLVSP
                     LNHVNMGQSTNDAVPTGARIALLTQLEGTIEGLDALACALEAKSEEFKDVVKMGRTHL
                     QDAVPITAGQEFGAWAKALRRDIERIEAASEGLHIINLGGTAVGTGLNVDIRFIHLVC
                     EELSKISGYPLQTAEDLVDCTQNVDVFANLSSCMKVCAVNLCKIANDMRLLASGPIAG
                     LHELLLPPVQPGSSIMPAKVNPVIPEVVNQVAFQIIGNDLSVTMAAQAGQLELNVFMP
                     VLLFNLLQS"
     gene            1674..>3395
                     /locus_tag="PLM83_08970"
     CDS             1674..>3395
                     /locus_tag="PLM83_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOK71568.1"
                     /translation="MAKSHWRKTPWIALLIMSLALALATAVGAAQSTPSGLPRGGWQP
                     GPDIYHPSRVIVRFSDVVSSEHATQSIAALGYSVDRVADFGVSANFPHGLRIGIVELP
                     DGVSPDTAVDRLSKTPGILYAEPDYIRYKDQAYGDAPIFPNDPQFNKMWGLHNQNCQF
                     KDPRMSGDPVDDADIDAPEAWGICTGSEETVVAIIDTGCYILHPDLAPNIWVNPGEIP
                     GNGIDDDGNGYIDDIWGWDWFNGDNSVWDPGERDQYGYLNDEHGTHTSGTIGAITNNA
                     IGVAGINWNIKIMILKFLGPEGGYTSDAILAFNYAANKGAKVISCSWGGGGYNQALKD
                     AIEATHAIVSCAAGNSGQNTDINPHYPSSYDSANIISVAAMMQNDTPCNYPGWWSTCW
                     GPTSVDLYAPGGYILSTIPPDPPPSQPGEAYDFFYGTSMATPHVSGAAALLHSLRPGL
                     PLYPSAPGSIPGQQTVKDILLNTVDVKPAYEGNVVSNGRLNIAAALLELGGPVITSID
                     AQPRFGPPPLEVTFTASAISPGGEIVDKWWDFGDGSTPVHEFNAVHTYTEQGDFTATF
                     HAVDGEGL"
BASE COUNT          815 a         1008 c          854 g          718 t
ORIGIN      
        1 cagattgcaa gagattgaag agcagcacag gcatgaatac attgagttca agctggcctg
       61 cctgggcagc catagtaacc gatagatcat ttcctatgat ctgaaatgcg acttggttca
      121 caacctcagg tatcacaggg ttaactttcg ccggcattat tgatgaaccc ggttgaacag
      181 gaggaagcaa cagttcatga aggccggcta taggtccgga ggccaagagc ctcatatcat
      241 tcgctatctt gcagagattg accgcgcaca ccttcatgca cgacgaaaga ttggcgaata
      301 cgtccacgtt ctgagtgcaa tccaccaagt cttccgcagt ttggagagga taaccgctta
      361 tcttcgagag ttcctcgcag acaagatgaa tgaaccttat atccacattg agcccagtgc
      421 ctaccgccgt tccgcccagg ttgatgatgt gaagaccttc actggccgct tcgatccgct
      481 caatgtcgcg cctcagtgcc ttcgcccaag ccccaaattc ctgccctgcg gtgataggca
      541 ccgcatcctg caagtgggtc ctccccatct ttacaacgtc tttgaactct tcggacttcg
      601 cctcgagcgc acacgcaaga gcgtccagtc cttcaattgt cccttcaagc tgtgtaagca
      661 gagcaatccg cgcccccgtg ggcaccgcat cattcgtaga ctgccccata ttgacatggt
      721 tcaaagggct cactaaagag taatcgccct ttaccccgcc gaggatctct atggctctgt
      781 ttgcaagaac ctcgtttaca ttcatgttga tcgatgttcc agctccgcct tggattgggt
      841 caagcatgaa actgctgtcg agcttcccag ccaaagcgtc gtcggcggcc tcagctatag
      901 cctgcccgat tcggctcggg atctggccta acttgctgtg ggcaagagcc gtcgccttct
      961 taaccatgat caacccacga ataagtgccg ggtgaaccga aagccctgtg atctgaaagt
     1021 tttcttgccc gcgaagagtc tgtatcccgt agtacgcgtc gaccggcacc tgccttttcc
     1081 ccagcgaatc cgtttcaata cgcattttgc catccgcaga tgtcccttgc ttcgacatgc
     1141 cactatctgt gcctgcattc cctgaaacaa cccttgtatc accattgtca ctaaccactg
     1201 acatccctga cgctccctat taagcatttt cggcaaccgg tccgtcgctt cagccagccc
     1261 ccaccaccca ccctctcgat aaccaccgtt ctgtttaaac aggagcgagc cagctatcgg
     1321 acaagcccat tacttgttca acttttctta atggaatcct tctagcgtgt ccacaagtac
     1381 gcccattccg atcagctcat gcaaaccgca gaggaattcg tcctaattcg tttattatca
     1441 taattatcca tagttggcag gattggggcc gcaatatgta gtatttaggg atttgcgggc
     1501 accgcacaga attcgtatgg cagctgattg ccaaacccag aaaggcagtt ttagcctgcc
     1561 cggaccaagg gggtgacgag cgggacaagc agtattaacg ttttcacgtc cacttcaggt
     1621 ttgcgttccc aaacacaatg aaatcatcag catgaaaggg gagtaatcga catatggcca
     1681 agagccactg gcgaaagact ccatggattg ccctactgat catgtcgttg gctctagccc
     1741 ttgcaaccgc agtaggagcg gcgcagagca cgccctcagg cttgccgcgg ggcgggtggc
     1801 aaccgggtcc cgacatctac catcccagca gggtaatcgt gaggttctct gatgttgtat
     1861 cctcggaaca tgcaacccaa tccattgctg ccctcggcta ttctgtagac cgtgttgcgg
     1921 atttcggcgt cagcgccaat ttcccgcacg gactgagaat cggcatagtg gagcttccag
     1981 acggcgtaag ccccgacaca gcagtggaca gactgagcaa gaccccgggc atactgtacg
     2041 ccgaaccaga ctacatccgc tacaaggacc aggcatatgg ggatgcccca atcttcccca
     2101 acgatccgca attcaacaag atgtggggtc tgcacaacca gaactgccag ttcaaggatc
     2161 cgcgcatgtc gggcgatcct gtagatgacg ccgacataga cgccccagaa gcatggggca
     2221 tatgcaccgg gtccgaagaa acagtcgtag ctatcataga caccggatgc tacatactac
     2281 atccagatct cgcaccgaac atctgggtca acccaggcga gatccccggc aacggcatcg
     2341 atgatgacgg aaacggttac atcgacgaca tctggggatg ggactggttc aatggcgaca
     2401 actccgtatg ggatccaggc gaacgcgacc aatacggata tctcaatgac gaacacggaa
     2461 ctcacacatc cggcaccatc ggcgccatta caaacaacgc gattggcgtc gccggcatca
     2521 actggaatat caagatcatg atcctaaaat tcctcggccc ggaaggaggc tatacctccg
     2581 atgccatcct tgctttcaac tacgcggcca acaagggcgc gaaggtgata agctgcagct
     2641 ggggcggggg cggctacaat caggcgctca aagacgccat agaggcgaca catgctatag
     2701 tgtcgtgcgc tgccggaaac agcggacaga acacagacat aaacccgcac tatccatcga
     2761 gttacgacag tgccaacatt atatccgtgg ctgctatgat gcagaacgat accccatgca
     2821 actacccggg ctggtggagc acgtgctggg gtccgaccag cgtcgacctg tacgcgcctg
     2881 gcggatatat cctctcaact attccgccgg atcctcctcc atcgcagcct ggagaggcat
     2941 atgacttctt ctacggaacg tcgatggcaa cacctcacgt gtcaggcgcg gcagccctgt
     3001 tgcactccct caggccaggc ctgcccctgt accccagtgc gccgggatca atacccgggc
     3061 aacagacagt caaggacata ctccttaaca ctgtcgacgt gaaacccgcg tacgagggca
     3121 acgtagtaag caacggcaga ttgaacatag ccgcggcgct cctcgaattg ggcgggccgg
     3181 tgataacctc gatcgatgcc cagcccaggt tcggcccgcc gccgctggag gtcacgttta
     3241 ccgcgtctgc gatttcgcct ggtggcgaga tcgtggacaa gtggtgggac ttcggcgacg
     3301 gtagcacacc agtgcacgaa ttcaatgctg tgcacacata cactgagcaa ggcgatttca
     3361 cggccacatt ccacgcggtc gacggcgagg gtctt
//
LOCUS       DAOSAI010000287         3394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17817_length_3394_cov_3.424978, whole genome shotgun sequence.
ACCESSION   DAOSAI010000287 DAOSAI010000000
VERSION     DAOSAI010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17817_length_3394_cov_3.424978"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PLM83_08975"
     CDS             <1..646
                     /locus_tag="PLM83_08975"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HOK71569.1"
                     /translation="DAVISLGPQHLSAYCLSVEEGTVLARAIASGVQTVPDDDLVAEF
                     YEWLVSRVENAGYVRYEVSNFAIPGFESVHNIRYWTCGYYVGLGVAAHSHLPAGAGGS
                     VRSWNVESVDKYVASISEGTRALSGFEERTAFEEAQDRLMLGLRMSEGIDLASIGRLY
                     GEDLLGEYGARFADLAEKGLVNFSGGKVRLTPRGFLLGNLVFSECVDCGDSAQS"
     gene            769..1821
                     /gene="hrcA"
                     /locus_tag="PLM83_08980"
     CDS             769..1821
                     /gene="hrcA"
                     /locus_tag="PLM83_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOK71570.1"
                     /translation="MAENMADRKKAILRAVTDDYISTAEPVGSRTIARKYGLGVSSAT
                     IRNEMADLEEEGYLEQPHVSAGRIPSDKGYRFYVDSLMPERSLTGLEGKKIRAECAKR
                     SDEIQSLIRATAKVLGELSNCASIVIGPGVKTAEIRHIQLVPFSSDSILVLVVTNAGL
                     VEHKILSIEHEMNPVDLENMSRMLNHRLRGRSLSQMRGKVLRDILSDLDAYASFLEQM
                     FELLLSAIRSSDENRVYVDGLMKLLGQPEFSEIERARPILEFLESDDLVLTELSGSAS
                     RGLGGAQVTIGSENTREELRECSVICAPYGIRGQVVGTIAVVGPTRIDYSLTIALVAH
                     IAESLSDLLTRTQSIK"
     gene            1835..2989
                     /gene="dnaJ"
                     /locus_tag="PLM83_08985"
     CDS             1835..2989
                     /gene="dnaJ"
                     /locus_tag="PLM83_08985"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOK71571.1"
                     /translation="MFATGNRDYYEVLGVERNASDEEIKKAYWDLARKWHPDVNPNDS
                     SAEAKFKEINEAYQVLSDPQKRARYDRYGREDGVAGGGWSQDFGGFDPFGSIFDAFFG
                     DSPFTRAAYPESRGPVRGHDLKATVEITLEEAATGVNKEISVDRMTMCERCGGEGMEP
                     GTSRTTCPECAGRGQVHSMSRTAFGTFTRVTTCPKCRGEGTIVDFPCNDCGGAGCVKK
                     TGRIEVDIPAGVDSGSRVRIRGEGDAGLRGGPPGDLYVYIKVKPHSVFKRDGNDLRIE
                     VPVSFPQLALGGEIVVPTIDGPKMTINIPRGTQTGKEFRQRGKGMPILRGFARGDMIV
                     TVRVVTPQKLSDREEELLRELAQIQGASAGETGGKKQSRWGKVLHRRDKS"
     gene            3004..>3394
                     /locus_tag="PLM83_08990"
     CDS             3004..>3394
                     /locus_tag="PLM83_08990"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOK71572.1"
                     /translation="MIWADVSCEVSDEDSEMVAEVFRMAGAGGVAFTGPSLITDSMAG
                     AESSEAALYPEGACFGGPTRVIAYFPVDDLLERRIGTIKRGLEEMWALKGGCLPCPTI
                     MLRQVENSNWAAAWKSHYKVEHVGDRIV"
BASE COUNT          784 a          728 c         1117 g          765 t
ORIGIN      
        1 agatgctgtg atatcccttg gaccgcagca cctgtctgca tactgtcttt cggttgaaga
       61 gggcactgtg ttggctcgag ctatagcttc gggcgtgcag acggtgcccg atgacgactt
      121 ggtggccgag ttctatgagt ggctggtttc ccgtgtggaa aatgcagggt acgtaaggta
      181 tgaggtctcg aattttgcga ttccaggctt cgaaagcgtg cataacattc ggtactggac
      241 ttgcgggtat tatgtcggac ttggggtggc tgcccattcg catctgcctg ccggggcggg
      301 gggatcggtc aggtcgtgga atgtggagtc ggtggacaag tacgttgctt cgatatccga
      361 agggacgcgg gcgctgtctg ggttcgagga gcgaacggct ttcgaggaag cccaggacag
      421 attgatgttg gggttgcgca tgtcggaagg gattgacctc gcttccatag ggcgtcttta
      481 tggggaagac ttgttgggag agtacggcgc aaggttcgca gacttggcgg aaaaagggct
      541 tgtcaacttc tctggtggca aagtgagact tactcctaga ggatttctcc ttgggaacct
      601 ggtgttctca gagtgtgttg actgtggaga tagtgctcag tcttgacagg gccgaactgt
      661 gatgttatgt tgtttttagc actcgactac tgtgagtgct aaacgacctg ggttatcgtg
      721 gcgttggggc gtgcagaggc tggtaaaagt gagagtgggg tgagagcgat ggctgagaac
      781 atggcggaca ggaaaaaggc tatactgcga gctgtgaccg atgactatat atcaacggct
      841 gaaccggtgg gctcaagaac catcgccaga aaatacggac ttggagtcag ttctgcgacg
      901 ataaggaatg aaatggctga cctggaggag gaaggttatc tcgagcagcc acatgtttcg
      961 gcaggcagga ttccgtccga taaaggttac cggttctacg ttgactctct catgcctgag
     1021 cggagtctga ctggcctcga gggaaaaaag atcagggcag aatgcgcaaa gcgcagcgat
     1081 gagatacaat cattgatcag ggccactgcg aaggtgttgg gagagctttc gaactgtgcg
     1141 tctatagtga taggtccggg agtgaaaacg gccgagattc gccacattca gttggttcct
     1201 ttcagttccg atagcatatt ggtgctagtc gtcaccaacg caggtcttgt ggagcataaa
     1261 atcctttcga ttgagcatga gatgaacccc gtcgaccttg agaatatgtc gcggatgctc
     1321 aaccacaggc tgcgcggaag gtcgctttcg cagatgcgtg gaaaggtgtt gagggatatc
     1381 ctgtcggacc tggacgctta cgcatccttc ctggaacaga tgtttgagct gttgctctct
     1441 gctatcagat catcagatga gaacagggtt tatgtggacg gcttgatgaa gctcctcggc
     1501 caaccggagt tcagcgaaat cgaacgcgcc aggccgattc tggagtttct tgaatcggat
     1561 gacctggtat tgactgaact ttcagggtct gcgtccaggg ggctgggtgg ggcgcaggtg
     1621 actatcggtt ctgaaaacac aagggaagaa ctgagggaat gcagcgtgat ttgtgcccct
     1681 tacggaatac gcggccaggt ggtgggcacc atcgcagtag tggggccaac taggattgac
     1741 tactctctta cgatagcatt ggtggctcac atagcggaaa gcctttcgga tttgctgact
     1801 cgaactcagt caattaaata gcgcggacga ggcggtgttt gctacgggca accgggatta
     1861 ctatgaggtt ttaggggttg aaagaaacgc atctgatgag gagataaaga aggcatactg
     1921 ggatcttgcc cgcaagtggc acccggatgt gaaccccaat gactcctcgg ctgaagcgaa
     1981 gttcaaggag attaacgaag cctatcaggt gctctctgat ccccaaaagc gagctaggta
     2041 tgatagatac ggacgtgagg acggagttgc cggcggtgga tggagccagg attttggagg
     2101 atttgacccg ttcggcagta tttttgatgc atttttcggc gattccccgt ttacgcgtgc
     2161 tgcgtatcca gaatcgcggg gacctgtgag agggcatgac ctcaaggcaa ctgtcgaaat
     2221 aacgctggag gaagcggcca caggggtcaa caaggagatc agtgtcgaca ggatgaccat
     2281 gtgtgaacga tgtggcggcg aggggatgga gccgggaact tctcggacga catgtcctga
     2341 atgcgcagga cgcggacagg tccattcgat gagcagaacg gcttttggca ccttcaccag
     2401 ggtaacaacg tgtcctaagt gccgtggaga ggggacaatc gtcgactttc catgcaatga
     2461 ttgtggaggt gctggttgcg tcaaaaaaac agggcgcata gaggtggata tccccgcagg
     2521 agtggacagc ggatcgaggg tgcggatcag gggtgaaggc gatgcgggac tccggggagg
     2581 cccccccggc gatctgtacg tctatattaa agtaaagccc catagcgtgt ttaagcgaga
     2641 cggaaacgat ctaagaatag aagtgcctgt ttcattcccc caactggccc tgggcggcga
     2701 gatcgttgtt ccgactatcg acgggcctaa gatgacgatc aacatccccc gaggaactca
     2761 gacgggtaaa gagtttcgcc aacgtggcaa agggatgcca atcttgcgcg ggtttgccag
     2821 gggagatatg atcgtgacgg tgcgagttgt tactccacag aaactgagcg acagagaaga
     2881 ggagcttctt cgggaacttg cacagatcca aggcgccagc gccggtgaga ccgggggcaa
     2941 aaagcagtct aggtggggga aggtacttca cagacgggac aagtcgtaag gcggtgtatc
     3001 gatgtgattt gggctgatgt ctcgtgtgag gtatcggacg aagattccga aatggtggct
     3061 gaggtatttc gaatggcggg ggcgggtgga gtggctttca ccggcccctc attgattact
     3121 gatagcatgg ccggtgcgga gagtagcgag gcggcgttgt accccgaagg tgcgtgcttt
     3181 ggcgggccca cgcgggttat agcttatttc cccgtcgatg atctcctcga gcggcgaatc
     3241 ggtaccataa agaggggcct tgaagagatg tgggccctaa agggcgggtg tctaccgtgc
     3301 ccaactatca tgctgaggca ggtggagaac tccaactggg cagcggcgtg gaagtctcac
     3361 tataaagtgg agcatgtggg cgatagaata gttg
//
LOCUS       DAOSAI010000288         3381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17922_length_3381_cov_3.222489, whole genome shotgun sequence.
ACCESSION   DAOSAI010000288 DAOSAI010000000
VERSION     DAOSAI010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17922_length_3381_cov_3.222489"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLM83_08995"
     CDS             complement(<1..784)
                     /locus_tag="PLM83_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71573.1"
                     /translation="MTTKGSILSTVGLSKHFSGVKALDKLDITVGEGQIHGLIGPNGA
                     GKSTFFNVISGLLPPSEGEVYFNSVNTTNIPAHTIARMGLGRTFQAARMMADTVLENV
                     MAGMYYYCETDPIRSFILQPRVFRTQEREIRQKAMEALDFLGMKDSAHRWADDLVWVE
                     RQLVQIARALVLDPKLLLLDEPTSGMGIMETEEMKGVIRHVRDSGVTVILVSHDVSLV
                     VDVCDWVTVINFGEKISEGNPRQIQNDPKVMEAYLGEEECVES"
     gene            complement(1406..2410)
                     /locus_tag="PLM83_09000"
     CDS             complement(1406..2410)
                     /locus_tag="PLM83_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOK71574.1"
                     /translation="MSVHDITRIRGLLVGHAQDEAALTGCTVVLTPGGAVAGVDVRGS
                     APGTRETDLMRPCNQVERIQGVVLAGGSAYGLDAASGVMKFLEEEGVGFSTPFGIVPI
                     VGAAVIFDLGIGDPKVRPDAAMGYAACRAAYSGPKGEGNIGAGAGATVGKIGGQEYAM
                     KGGLGTYVAELQVDPGGEAEPVLVGALVVVNSLGDVINPWSGQIVAGAYDRSSKTFIG
                     PGASRNHVNTVQVPDRLGTNTTVAVVATDAVLDKEGANKVAQMAQNGLARAIVPAHTM
                     YDGDTVFCLSTAEKCLSGDRAADITTIGSVAAQALAAAVVRAVRAASSVPGCLAVNDI
                     "
     gene            complement(2456..>3381)
                     /locus_tag="PLM83_09005"
     CDS             complement(2456..>3381)
                     /locus_tag="PLM83_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOK71575.1"
                     /translation="FAEQAQGAEGCRVEAELLLRHPDVKGVSYVGSSVVGRHIYATAA
                     ANQKRVQCLGEAKNHGLVLRDANLEMAARVIVNSSFGCAGMRCMALPVVVVEEEVADE
                     FMSYFVQFARERKIGCAYDIATELGPVVSAEHKDSVIAWIEKGIEEGAELVLDGRDVI
                     VQGFEGGYFLGPTIFDHVMPGMTVGDSEIFGPVACVKRVKDFEEGLALMNANPFANGS
                     CIFTESGHWAREFMRRTHGGMVGINVGIPVPISVFPFCGHKESFYGDLHVMGTDGMAF
                     YTETKSVTTRWLGTAEAVKSVSTWEGTITRE"
BASE COUNT          768 a         1050 c          762 g          801 t
ORIGIN      
        1 aactctcaac acattcttct tcaccgagat aagcctccat taccttcgga tcgttttgta
       61 tttgtcttgg atttccttcg cttattttct ctccaaagtt gatcacggtg acccagtcac
      121 agacatccac taccaaactg acgtcatgac tgaccaggat tacggttact ccgctgtccc
      181 tcacgtgcct gataacgccc ttcatttctt ctgtttccat aattcccatt cccgaagtag
      241 gctcatccaa aagcagcagc ttcggatcaa gcaccaatgc acgggctatc tgtactaact
      301 gacgttctac ccaaacgagg tcgtcagccc aacgatgcgc agaatctttc attcccaaaa
      361 agtccaaggc ttccatggct ttttgcctga tctctctttc ctgagttcga aaaacgcgag
      421 gctgcagaat aaatgatctt atcggatcag tctcacagta gtaatacatg cctgccatca
      481 cattctcgag aacagtgtca gccatcattc gtgcagcctg aaacgtccga ccaaggccca
      541 tcctggcaat ggtgtgcgca ggaatgttag tcgtgttcac tgaattgaag tagacttcgc
      601 cttcgctcgg cggcaatagc ccagagataa cattgaaaaa cgtggattta cctgctccat
      661 tagggccaat cagaccatgg atttggccct caccaacagt aatgtctaat ttgtcgagcg
      721 ctttgacgcc gctgaaatgc ttgctcagcc cgacagttga gaggattgac ccctttgtag
      781 tcatcccaat caaagctcct ctgctttcca aatcgttagt tattgcttct agcctgtttt
      841 ggttgtcttc ccatttcgac aggcaggtac tttctcctgc cgtatttgga aaccgtgttt
      901 ctatttctga cacatgtgag ctgtgcgagc aaggactttt gctcaggtgg gatgtgcatt
      961 tctactacac ttacatttcc aagcttattc agattaacga agatcaattg ccataccgcc
     1021 actaatcatg ggcattagta agcagcttct tcttaaattc caatcggtct taccgtttct
     1081 attgccgaca tgtctgggga ataagttgtt caatgtctag aatggaaata tcgatatctc
     1141 ttcttatgtg cacagaagtc ctttagcacc acacaaaagc cgatgttctc tgatacttgg
     1201 ctccacggac caggaagcgg aatcatcttg ttgctctaac aaagctagac accgtcatac
     1261 aatcaaccac agacagataa ttgcatagag caatattgta gttctccacc ggatgttctc
     1321 ccaataagcg cttgaaatct gacgcatcca gctcagtcgc aggctcctgt gttaccctcc
     1381 ctcattacct caagccttgc aaagcttaga tatcgttcac cgccagacac cccggaacag
     1441 aactcgcagc cctaactgcc ctgaccaccg ccgctgcaag ggcctgagcg gctacggaac
     1501 caatggtggt gatatccgct gccctgtccc ccgaaaggca cttttcggcc gtggacaagc
     1561 agaatactgt gtccccgtcg tacatggtgt gcgccggtac aatcgccctg gcgagcccgt
     1621 tttgggccat ctgggcaact ttgttggcac cctctttgtc caggaccgca tctgtggcca
     1681 caactgcaac agtggtgttg gtgccgagtc tgtccgggac ttgcacggta ttcacatgat
     1741 tccggcttgc gccgggacca atgaaagtct tgctgctccg gtcatacgct cccgccacga
     1801 tctgccctga ccagggatta atcacatccc ccagggaatt gacgactacc agggccccaa
     1861 caagcaccgg ttccgcctcc ccgccggggt cgacttgaag ttctgccaca tatgtcccga
     1921 gcccgccctt catggcatac tcctggccac caatcttgcc tacggtggcg ccagccccgg
     1981 cgccgatgtt cccctcaccc ttcggccccg agtaggcagc gcgacacgcc gcataaccca
     2041 tagcggcatc gggacgaact ttgggatcgc ctatccccag atcgaaaatg accgcagcac
     2101 ccacaatagg cacgataccg aaaggtgtgg aaaagccaac tccctcttcc tcgaggaact
     2161 tcatgacgcc ggacgcagca tccagaccat aggcgcttcc tcccgcaagg accacgccct
     2221 gaattctctc cacctgattg catggacgca tcagatcggt ctcacgtgtc ccaggcgccg
     2281 agcccctgac gtcgacacca gccactgctc cacccggcgt cagcacgact gtgcagccag
     2341 tcaacgccgc ctcatcctgg gcatggccca caagcaaacc tcgaattcgg gttatgtcgt
     2401 gcacgctcaa tcgcctccag tcgccctggc cgaccctggc gcagggtttg cctgtctatt
     2461 cgcgcgtaat ggtcccttcc cacgtggaga cgctctttac agcctcggct gtccccagcc
     2521 atctggtggt cactgacttg gtttcagtgt aaaaggccat tccatcagtt cccattacgt
     2581 ggagatcacc atagaacgac tccttgtgcc cgcaaaaggg aaagactgag atcgggacgg
     2641 gaattcctac gttgatgccg accatgcctc cgtgagtacg ccgcatgaat tcccgggccc
     2701 aatgcccact ctccgtaaag atgcaagatc cgtttgcaaa cgggttggca ttcatcagcg
     2761 ccaggccttc ctcgaagtcc ttcacccgct ttacacaggc tacggggccg aatatctcgc
     2821 tgtcgccgac tgtcattcct ggcatcacat ggtcaaatat agtgggtccc aggaaatatc
     2881 cgccttcaaa gccctgcact atgacatctc tcccatcgag caccaactca gcaccttctt
     2941 cgatgccctt ttcgatccag gcgatcacgg aatccttgtg ctctgcagat actacgggac
     3001 caagctctgt ggcaatgtcg taggcacaac cgatcttccg ctccctggca aactggacga
     3061 aataggacat gaactcgtcg gcaacctctt cctccacgac cacaaccgga agagccatgc
     3121 atctcatccc ggcacaacca aatgaggagt tgacgatcac acgcgccgcc atctcgagat
     3181 tggcgtctcg cagaacgagt ccatggtttt tcgcttcgcc caggcactga acgcgcttct
     3241 ggttagcggc tgccgtagca tagatgtgcc ggcccaccac ggaagaaccc acatacgata
     3301 ctcctttcac atctggatgc ctgagaagca gttcggcctc gacgcggcac ccttcggcgc
     3361 cctgcgcctg ctcggcgaaa g
//
LOCUS       DAOSAI010000289         3374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_17986_length_3374_cov_3.385658, whole genome shotgun sequence.
ACCESSION   DAOSAI010000289 DAOSAI010000000
VERSION     DAOSAI010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17986_length_3374_cov_3.385658"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLM83_09010"
     CDS             complement(<1..827)
                     /locus_tag="PLM83_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK71576.1"
                     /translation="MRKLCKSLGSVLLAIALMMCVGGSGALGAPSSKVVVDQWEIPFL
                     NCLTGPIASIGEYMAWSAHRAADEINQKGGVKGKPIKIVDLDTGVSPEKAIEQMAKIV
                     NKSLVALGPVPEACIMAAMPLAVKNGLLCMIASTTYEYQSQFFPYAVAWDSGSDKTLP
                     PVLAGWAQRNPGMKRVVQFVERWACWPGMADAHAAGLASVGVKTVEIEVPTDAVTFGP
                     LVVRALAQKPDGFVLTCTSEKAAKIIIELEKYGWTDKSKIIVFSSADDMPLYTTGRE"
     gene            complement(824..1705)
                     /locus_tag="PLM83_09015"
     CDS             complement(824..1705)
                     /locus_tag="PLM83_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOK71577.1"
                     /translation="MSPVIYYFLSFTAINILMCWAFYLPYRVGQLHHLVVSCMMASAY
                     FSGYAARQWSWPFALTLLVGTAIGMLICLLPSFAIGDAPCFTVVIVGLTVLFIMKTAI
                     ENTRALGGTLGFFGIPQVNNLLLLAYLCLAVAGLLIYRIDNSRLGRAASMVFVDRDAA
                     SALGVDVKRLGMLFQIIAGGLAGLAGSLYAFLIGSLFPEFFNLGVIGTLMCMLFAGGH
                     STMWGVVISVPVFEGLHLILPTAVASWLQVIYAVTLIVVILVLPGGLITKKMLVKVGE
                     RFRARPQTVSIKGGIKE"
     gene            complement(1762..2637)
                     /locus_tag="PLM83_09020"
     CDS             complement(1762..2637)
                     /locus_tag="PLM83_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOK71578.1"
                     /translation="MEIFVAQLLNGLSLGSIYVLLVLGFNLMLVVAKIIQFAYPHLVV
                     LSMYVCWGMLKYTNNMFLVVLASIVSCVIVNVAIEPIFRALSKKHGLDINVSFVVSIS
                     IALLITDVMSHALNYGFPIAFPTDWVGKSVLNFGLITFSVGQAYALIGGIAAVALFFY
                     LLYRTRQGRVFRALSENSVLASIIGIPVVKTRILSYAIAGLLGGVTAVLFARLLGSAS
                     PSLGDFVAMKVVAVSILAGLGNLKGGLVCGLLLGIAESLAMGYLPGSWSNAIAFMMIL
                     IIMLIKPEGLFGIQI"
     gene            complement(2667..3371)
                     /locus_tag="PLM83_09025"
     CDS             complement(2667..3371)
                     /locus_tag="PLM83_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71579.1"
                     /translation="MLRVEGISVSYGPIQALGDVSIEVDEGEIVSLVGANGAGKTSLL
                     RAIMGLQKVHTGRIVFMGNDITNTSTDKVAGSGIGLIPEGHLVFSTMTVLENLQVGGY
                     HYPQEVSSSLESVFESFPVLYKRRNQIAGTLSGGEQQMLSIGRTLMSKPKLLMADEPS
                     LGLAPKVISEIFNILRDLNEKGYPILLAEQNAKKALEFGDHAYVMETGRVLFQGPAKE
                     LMGNPKVRHAYLGGNV"
BASE COUNT          902 a          934 c          787 g          751 t
ORIGIN      
        1 ttctctcttc cggtggtgta gagtggcata tcatctgcgc tgctgaagac gattatcttg
       61 gacttgtccg tccacccata cttctccagc tcaatgatta tctttgcggc cttctccgat
      121 gtgcacgtaa gcacgaaccc gtcgggcttc tgagcaagag cccttacgac aagagggcca
      181 aaggtgaccg catcagttgg cacctcaatt tcgacagtct taactcctac tgaagccaag
      241 cccgcagcat gagcatccgc cattccaggc caacatgccc atctctcaac aaactgcacg
      301 acccgtttca ttccgggatt tcgctgggcc cagccagcga gaaccggagg taaggtcttg
      361 tccgatcctg aatcccatgc aacagcgtaa gggaaaaact ggctctggta ttcgtaggtt
      421 gtagacgcga tcatgcagag taggccgttc ttcactgcca acggcattgc cgccattatg
      481 caggcctccg gaacagggcc tagtgccaca aggctcttgt tgacgatttt ggccatctgt
      541 tcaattgcct tttccggact gactccagta tcaagatcca cgatcttgat aggcttccct
      601 ttaactccgc ccttctggtt gatctcatcc gcagctctat gagcgctcca cgccatatac
      661 tcaccgatac tggcaatagg acctgtgagg cagttcagga aaggtatctc ccactgatcc
      721 accacaactt tgctagaagg tgcgcccagg gcgccggaac cgcccacaca catcatgaga
      781 gcgatcgcca ataacacgct acccagcgac ttgcaaagct ttctcactct tttattcctc
      841 ccttaataga tacagtctgt ggtcttgcgc ggaacctctc tccaaccttg accaacattt
      901 tcttggttat cagtccgccc ggcaatacca gaatgacgac gataagtgta actgcgtaaa
      961 tgacttgaag ccatgaagcg acagcggtgg gaagaatcaa atgcagacct tcgaaaacgg
     1021 ggactgagat taccaccccc cacatagtgc tgtgcccgcc tgcaaacagc atgcacataa
     1081 gggtgcctat gacacccaga ttaaagaact ccggaaacag actgcctatg agaaacgcgt
     1141 aaaggcttcc tgcaagtccg gcaagcccgc ctgcaatgat ttggaacagc atgcccaacc
     1201 gcttcacgtc gactcccagc gcactcgcgg catctctgtc tacaaagacc atggatgccg
     1261 ctcttcccag ccgggaattg tctatgcggt aaatgagcaa tccggcaacc gcaaggcaaa
     1321 gataggctag tagtaataag ttattaacct gaggaatgcc aaaaaagccc aatgttccac
     1381 ccagggcacg cgtgttctca atagctgtct tcataataaa caggaccgtt aggccgacta
     1441 tgaccacagt gaaacaggga gcatcgccaa tggcgaacga gggaagcaga cagatcaaca
     1501 tgccaatggc tgttcccacc aggagtgtga gcgcaaatgg ccacgaccac tgccgcgccg
     1561 catagccgga gaagtaggcg gatgccatca tgcaggaaac gaccaagtgg tgtagctggc
     1621 caactcggta tggcaagtag aacgcccaac acataaggat atttatggcc gtgaatgaca
     1681 ggaaataata tattacaggg gacaattttc cagcctcctt tgtcgttgtc ggattgacag
     1741 gacagaaata tagtgtagag gctatatctg aatgccaaac aacccttcag gctttatcaa
     1801 catgattatc aagatcatca tgaaggcaat ggcatttgac caggaccccg ggagatatcc
     1861 catggccagg ctttcggcta ttccaagaag cagtccacat acgagacccc ccttgagatt
     1921 accgaggcct gccagtatgg aaacggccac taccttcata gcgacaaaat caccaaggct
     1981 cggggaggct gaacccaaca gtctggcaaa gagcacagcc gtaacgcctc ccaagagacc
     2041 tgctatggca taactcagaa tccgcgtctt gacaacaggt atgcctatga tagacgcaag
     2101 gacgctgttt tcgcttaatg ccctgaacac tctgccttgc cttgtccggt acaggaggta
     2161 gaaaaagagc gcaacagcag caatgcctcc gatcagagcg tacgcttgcc cgacagaaaa
     2221 ggtgattaat ccgaagttca gtacgctctt ccctacccag tctgttggga aggcgatagg
     2281 aaacccgtag ttaagcgcat gggacatcac atctgtaatc aaaagagcta tgcttattga
     2341 cacgacaaaa gaaacgttga tgtcgagtcc atgcttcttc gaaagagcgc ggaatatggg
     2401 ctcaattgca acattcacta tgacgcagga tacaatactg gcgagcacaa ctagaaacat
     2461 gttgttggta tacttcagca tgccccagca gacatacata gagagcacca ctagatgggg
     2521 atatgcgaat tggatgatct tcgccacaac gagcatcaag ttaaaaccta gcaccagcag
     2581 aacataaatg ctacccaggg ataggccatt tagtagctga gccacaaata tctccatagc
     2641 gcgtcgtctc cttaacttta gtcatgctac acgttgccgc ctaaataggc atgtcgcact
     2701 ttcggattgc ccattagttc cttcgcgggg ccctggaaca gcactctacc agtctccata
     2761 acgtaggcgt gatcgccaaa ctctagcgcc ttcttggcgt tctgctccgc aagaagaatc
     2821 ggatacccct tttcgttcaa atcacgcaaa atgttgaata tctcgcttat cacttttgga
     2881 gcaaggccca gggaaggttc gtccgccatg agcaatttgg gctttgacat gagagttcgg
     2941 ccgatcgaca gcatctgctg ttctcccccg ctaagggtgc cggcaatctg attccttctt
     3001 ttatagagga cagggaacga ctcgaaaaca ctctccaggc tgctgctgac ttcctgagga
     3061 taatggtagc cccctacttg cagattctcc aagacagtca tagtggaaaa aaccagatgg
     3121 ccctcgggta tgaggccgat accggaacca gccactttgt ccgtgctcgt attggtgatg
     3181 tcattcccca tgaagacaat cctgccggtg tggaccttct gaaggcccat aatcgctctg
     3241 agcaatgagg ttttgccggc gccattggct ccaaccaagg atacaatctc tccttcatca
     3301 acctcaatgc tgacatcacc tagcgcctgt attggcccat aggatacaga gatgccttca
     3361 actctcaaca catt
//
LOCUS       DAOSAI010000290         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_18008_length_3371_cov_4.568154, whole genome shotgun sequence.
ACCESSION   DAOSAI010000290 DAOSAI010000000
VERSION     DAOSAI010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18008_length_3371_cov_4.568154"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLM83_09030"
     CDS             complement(<1..850)
                     /locus_tag="PLM83_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HOK71580.1"
                     /translation="MTKKMRVVHYLNQFFGQIGGEDKAGIAPFDKPGHVGPGMALAAA
                     FGDEAEIVGTVICGDTYFGENMEKATEEVVALIEKYKPDLLVAGPAFNAGRYGTACGA
                     VCAAVAEKFGIPVVTGMYPENPGVDMFRKNAYIIETGDSAAGMRKAVPQMAKLALKLA
                     KGEPTDPATDGYLSRGIRENYFAEKRGSERAVEMLVKKLSDKDFVTEYPMPEFDRVEP
                     NPPIADISKATIALVTSGGIVPKGNPDHIESSSASKFGKYDLEGVEALTSETHETAHG
                     GYDPVYA"
     gene            complement(947..1414)
                     /gene="grdA"
                     /locus_tag="PLM83_09035"
     CDS             complement(947..1414)
                     /gene="grdA"
                     /locus_tag="PLM83_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198010673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1286..1288),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HOK71581.1"
                     /translation="MILDGKKVVALGDRDGIPGGAIEECVKTAGGEVVFSVSECFVUT
                     AAGAMDIENQRRVKELAEKYGAENLVVILGGAEAEASGLTAETVTAGDPTYAGPLAGV
                     QLGLSVWHIVEDEIKAEIDPEVYDEQVGMMEMVLDVDAIKEEVSSIRKQYSKY"
     gene            complement(1493..2779)
                     /locus_tag="PLM83_09040"
     CDS             complement(1493..2779)
                     /locus_tag="PLM83_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOK71582.1"
                     /translation="MRLELGRIRIKDVQFGPATKVEDGVLYVDRAAIEAIVREDERIA
                     DVTVELAKPGEEVRIIPVKDVIEPRVKVEGAGGVFPGMVSKVETVGDGRTHVLSGAAV
                     VTCGKVVGFQEGIIDMTGPGADYTPFSKLNNVVVVCEPIAGLPQHEHEVAIRMAGLKV
                     AAYLGEAGRDIEPDEVDVYECLPLLEQVNAYPDLPKVVYVYMLQSQGLLHDTYVYGVD
                     AKKILPTLIYPTEVMDGAIVSGNCVSACDKNTTYHHQNSPVIHDLLARHGRDINFIGV
                     VITNENVTLADKERSSNFASKLVEMLGAEGAVVSEEGFGNPDADLIMNCVKLEKKGIK
                     TVLVTDEYAGRDGASQSLADANPLANAVVTAGNANEVVNLPPMKKVIGHPEAANIIAG
                     GWDGSLASDGSITAELQVITGATNELGFNKLSAREA"
     gene            complement(2807..3127)
                     /locus_tag="PLM83_09045"
     CDS             complement(2807..3127)
                     /locus_tag="PLM83_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOK71583.1"
                     /translation="MVQVDKVNFEEEVLQAKGPVLVDFWSTKCEPCMALLPDVEALEA
                     KYGDQIKFCKLNVLENRRLAISQKVLGLPTICIYKDGEKVAELTKDDATAANIEAMIK
                     EQIQ"
BASE COUNT          655 a         1050 c          839 g          827 t
ORIGIN      
        1 tagcatagac tgggtcgtat cccccatgcg cagtctcatg ggtctcactc gtcaaggctt
       61 cgacgccctc aagatcgtac ttgccgaact tcgacgccga agaggactca atgtggtcag
      121 ggttgccctt tggaacgatt cctccgctgg tgaccaaggc tattgtagcc tttgatatgt
      181 cagcaatggg gggattgggt tccacacgat cgaactcggg catagggtac tccgtgacaa
      241 agtccttgtc ggagagcttc ttcaccagca tctcgactgc acgctcagag ccgcgcttct
      301 cggcaaagta gttctcccgg atcccgcggg aaagatagcc atcggttgca ggatccgtgg
      361 gctccccttt tgccaatttg agtgcaagct ttgccatttg cggcacagcc ttgcgcatgc
      421 cagcggcact gtcgccggtc tcgatgatgt aagcattctt gcggaacatg tccactccag
      481 ggttttcagg atacatccca gtcacaaccg ggattccgaa cttctccgcc accgccgcgc
      541 acacagcgcc gcaagcggtt ccgtatcgtc ccgcattgaa ggctggccca gccacgagca
      601 gatccggctt gtatttctct atgagggcaa ctacctcttc ggtcgccttt tccatgttct
      661 cgccgaaata ggtgtcgccg catataactg tcccgacgat ctcagcctca tccccaaagg
      721 cagcggccaa cgccatgcca ggacccacat ggcctggctt atcgaaggga gctattcctg
      781 ctttgtcttc gccgcctatc tgtccgaaaa actggtttag atagtggact acccgcatct
      841 tcttggtcat tccgcttccc tcctcgcaag agggtttagc tcaacccgct gtaagcatca
      901 gtcagttccc gactcgctgc cgccgattgt ggcagcaggc cctggatcag tacttgctgt
      961 actgcttgcg tatagagcta acctcttctt tgatggcatc gacgtccagg accatttcca
     1021 tcattccaac ctgttcgtca tatacctcag gatctatttc cgcctttatc tcgtcttcca
     1081 ctatgtgcca aacactcagt cccaactgga ctcccgccaa aggacctgcg taggtggggt
     1141 caccggcggt cacagtctct gctgtgagcc ccgaagcctc agcctccgct cccccgagga
     1201 ttactacaag gttctctgcg ccgtacttct cggccagttc ctttactctc ctctggtttt
     1261 caatgtccat ggcgccggca gccgttcaga caaagcactc gcttacagag aagacaactt
     1321 cgccgccggc cgtcttgacg cattcttcaa tggcgccgcc tgggatacca tcgcggtcgc
     1381 ccagagcgac aacttttttg ccgtcgagaa tcattgcggc atctcctttc ccagcttctt
     1441 agttgaattt agcagtctca gctcagcaaa gccgtgacgc cctacggcaa actcacgcct
     1501 ctctagctga gagtttgttg aatcccagtt cgttggtggc tccagttata acctggagtt
     1561 ccgcagtgat cgagccgtcc gacgcgagag aaccgtccca accccctgcg atgatattgg
     1621 ccgcctccgg atgtccgatt accttcttca taggcggaag gttgaccact tcgttggcgt
     1681 tgccggccgt taccactgcg tttgccagag ggttagcgtc tgcaagagac tgcgacgcgc
     1741 cgtccctgcc ggcgtactcg tcggtaacga ggaccgtctt gatccccttc ttctccagct
     1801 taacgcagtt catgatgagg tcggcatcgg gattcccgaa tccttcctcc gatactacgg
     1861 cgccttcagc cccgagcatc tccactagct tcgaagcgaa gttggaggag cgctccttgt
     1921 cggccaaggt gacgttctcg ttggtgatca cgacgccgat gaagttgata tcgcgcccgt
     1981 ggcgtgctag gaggtcgtgt atgaccggcg agttctgatg gtggtaagta gtgttcttgt
     2041 cacacgccga cacacagttc cccgatacga tcgccccatc catcacctca gtcgggtaga
     2101 tgagcgtggg cagaatcttc ttggcgtcta ctccatatac gtaggtgtca tgcaacaacc
     2161 cctggctctg cagcatgtaa acgtagacta ccttgggcag atcgggatag gcattgacct
     2221 gctccaacag gggcaagcac tcgtagacat cgacctcgtc gggctcaata tcgcgccctg
     2281 cctcgcccag ataggccgct accttgaggc cggccatcct tatggctacc tcgtgctcgt
     2341 gctgcggcag gccggcgatg ggttcgcaga ctacaaccac gttgttcagt tttgagaacg
     2401 gagtgtagtc ggcgcccgga ccagtcatgt cgatgatccc ctcttggaag ccgacgactt
     2461 tgccacaggt cacgacagcg gctcccgaaa gaacatgagt gcgcccgtcg ccgacagtct
     2521 caaccttgct gaccattccg gggaacacgc cgcccgcgcc ttccaccttg acccgaggct
     2581 cgatgacgtc cttcacaggg atgatcctta cctcttcccc gggcttcgcg agctccacag
     2641 tgacgtcggc gatcctctcg tcctcgcgga cgatcgcttc gatagccgct cgatccacgt
     2701 agagtacgcc gtcctctact ttggttgcag ggccgaactg cacgtcctta atgcgtattc
     2761 tgccaagttc caaccgcatt tacttcacct ccagagcagg actctgctac tgaatctgct
     2821 ccttaatcat cgcctctatg ttggcagcag tcgcatcatc cttagtcagc tctgccacct
     2881 tctcgccatc cttgtagatg catatggtcg gaaggccgag aaccttctgg cttatagcca
     2941 gacgcctgtt ttcaagcacg ttgagtttgc agaatttgat ctgatctccg tatttggctt
     3001 caagtgcctc cacatcaggc agaagtgcca tacacggctc acacttggtg ctccagaaat
     3061 cgaccaaaac tgggcctttt gcctgcaaga cctcttcctc gaagtttacc ttgtctactt
     3121 ggaccatcct tgttcacctc cttaatctgc taagtgggcc cgatagtcaa ccgtcgaaag
     3181 ccgtctgctc aacctcactt gccggagatt accctgtcag ctaccagcgc gagttcctct
     3241 tcggttgtgg gccactgcac ccgctcgcaa acctgtcctt tattgaagaa gagtatcgaa
     3301 ggggcagccg atatctcgta cctgccggca atgcgctggt tcttgtatat gtctattttg
     3361 accaggttga g
//
LOCUS       DAOSAI010000291         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_18302_length_3331_cov_3.344933, whole genome shotgun sequence.
ACCESSION   DAOSAI010000291 DAOSAI010000000
VERSION     DAOSAI010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18302_length_3331_cov_3.344933"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLM83_09050"
     CDS             complement(<1..649)
                     /locus_tag="PLM83_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71584.1"
                     /translation="MIQSRQTQGQLESLLISVLIHAVIIFLVPYVAPAPTVDVYPLDV
                     AGVIEISAIETEPASPAPAPGLNVKEVAPTPASEPPKAAPTKKKDPKPAVAPAPAAKP
                     QPQPAAEPKPVGKTEPKVPTPAVDKPEVVSSPAGKSVAPASEPEPVPAAAPAQVPEPN
                     STLSKAVESPTASAKSYTEAKPASGSGTVGGTGEKPPTAGDPGGTGVVPRPEYPGD"
     gene            complement(646..1074)
                     /locus_tag="PLM83_09055"
     CDS             complement(646..1074)
                     /locus_tag="PLM83_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOK71585.1"
                     /translation="MFENHRAKRRLGHVIITPLIDVMFYLVAFLLVFGTFRTETTGMP
                     MELPRAATPSDLAREHIIVAIDSSGRIYYDERIVVDSELTTGLLPRLAANPDRLVIIK
                     ADKSVTYERLIQVIDAIRMAGGVHLALAVERAPQSGGVER"
     gene            complement(1074..1694)
                     /locus_tag="PLM83_09060"
     CDS             complement(1074..1694)
                     /locus_tag="PLM83_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOK71586.1"
                     /translation="MLKYIKAGGPVMYPIIFVSIIGLAVVIEKIIQLFHTQTDSDRIM
                     RSVKLNLAQGRPVEAMQAVKNEKGPIAGVARAAIANYDKPREQIRAAVEAAGREEVFM
                     MERKMNVLDGVITLAPLLGLLGTVTGLIKSFQVLSAFGGLTTPAQLSGGIAEAMITTA
                     AGLIVATPLVIIESWLSVVIDDRVADMEKYAIELIALAAGEDEGDQ"
     gene            complement(1789..2943)
                     /locus_tag="PLM83_09065"
     CDS             complement(1789..2943)
                     /locus_tag="PLM83_09065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOK71587.1"
                     /translation="MSKKSADIVVIGGGVVGCATAFELARLGCKDIVLVERNTIASGS
                     TGRCGAGVRQQFGTELNCVLARESVRIFEQMDEYLDYKPGVEFRQSGYLMLAYSDSQW
                     EQFRRNVKLERSLDIDVEMLTPEGAREIVPFLNLEGMVGATFCQTDGHANPFHTAFAY
                     AQAAKRLGAEVLTHTSVVDIEVENNRIKAVVTDKGRIETSAVVNCAGAYSAKIAAMAG
                     IDLPVWGERHQILITEPVEAMMGPMVISFHHDFYCQQTPHGSFIMGIGMPGEPKSFNI
                     HSSVDFLEGMAKTVMKILPPLGNLRVVRQWAGLYDRSPDAQPILGECPPVERFYNANG
                     FSGHGFMVAPIVGRAMAELIMLGKPSVDISKLDAGRFERGELVLEPAVVG"
     gene            complement(2936..3232)
                     /locus_tag="PLM83_09070"
     CDS             complement(2936..3232)
                     /locus_tag="PLM83_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOK71588.1"
                     /translation="MTDRPERIDDDVIVCRCEDVTLGDIRRLIANGYHTIDEIKRISR
                     AGMGQCQGRTCRHLIARELATALGVSVEEVEMPTFRPPTRPVLLSTIARGEGDE"
BASE COUNT          689 a         1005 c          842 g          795 t
ORIGIN      
        1 cgtcacctgg gtattccggc cgcggaacta ctccggttcc tccaggatct cctgcagtcg
       61 gaggtttttc acccgttccg ccgacagtgc ctgatcccga ggcaggcttc gcttccgtat
      121 agctcttggc cgatgcagtc ggcgactcga cagccttgct caatgtcgaa ttcggttctg
      181 gaacctgtgc gggcgcggca gcgggaacag gttcgggttc cgaagccggg gcaacgctct
      241 ttcctgctgg actcgaaaca acttcgggct tgtcaactgc gggcgttggg accttcggtt
      301 cggtcttccc gactggtttc ggctcagccg ctggctgagg ctgtggtttg gcggcaggcg
      361 ctggtgctac cgcaggttta ggatctttct tcttcgtcgg cgctgccttt ggaggctccg
      421 acgccggcgt tggcgcaact tccttcacat tgagccctgg cgcaggtgcc ggggaggcag
      481 gctctgtttc aattgcggat atctctatga cccccgcaac gtcgagcgga tacacatcca
      541 cggttggcgc aggcgccacg tacggcacca ggaatatgat caccgcatgg atgagcaccg
      601 atatcagcaa gctctccaac tgaccttgag tctgccgact ctggatcatc tctcaacacc
      661 gcccgattgc ggagcacgtt cgacggcaag agccaggtgg actccgccgg ccatcctgat
      721 cgcgtcgatt acctgaatga gtctttcata agtgactgac ttgtccgcct ttatgatgac
      781 gaggcgatca gggttcgcag ctagtctcgg caatagcccg gtggtaagct cgctgtcgac
      841 cacaatccgt tcgtcatagt agattcgccc gctcgaatcg atagccacga tgatgtgttc
      901 gcgagcaaga tcgctcggag ttgcagccct tggaagctcc ataggcattc cagtggtctc
      961 cgttcggaat gtgccgaaaa ccagcaagaa agcgactaga tagaacatga cgtcgatgag
     1021 cggtgttatt atcacatggc ctaaccttct ctttgcacgg tgattctcga acatcactgg
     1081 tccccctcat cttcgcctgc cgccaacgct atgagctcaa tggcgtactt ctccatgtct
     1141 gccactctgt cgtctatcac cactgagagc caactttcaa tgatgaccag aggcgtggca
     1201 acgatgagcc ctgcagcagt cgtgatcatc gcttccgcga tgccgcccga aagctgtgcc
     1261 ggcgtagtga ggcccccgaa cgccgacagg acctgaaaac tcttgatgag gccagtaacg
     1321 gttccgagaa ggccgagaag aggtgcaagc gtgatgacac cgtcgagcac gttcatcttc
     1381 cgctccatca tgaatacctc ttcccggcct gcggcttcta ccgccgccct gatctgttca
     1441 cggggtttat cgtaatttgc tatagcggcg cgggcaacgc ccgcgatagg ccctttttca
     1501 ttctttacgg cctgcattgc ctctacgggg cgtccctgtg caaggttgag tttgaccgag
     1561 cgcatgatgc gatccgagtc tgtttgtgta tggaaaagtt ggatgatctt ctcgatgacg
     1621 acggctagtc cgattattga cacaaaaatg attgggtaca tgactggtcc gccggctttt
     1681 atgtatttta gcatcgccat agctccccct ctaccgacaa atgcgcccgc ttgtgatgtt
     1741 tggacgctcc tccattcttc tacgttatct ggggaaattc ctctaggttt atccgactac
     1801 cgccggttcc aaaacgagtt cgcctcgttc aaatcttcca gcatctaatt tgcttatatc
     1861 gacagaaggt ttgccgagca tgataagctc tgccatagcc cgcccaacga tgggcgcaac
     1921 catgaaaccg tgccccgaaa aaccgttggc attgtagaaa cgctccacag gcggacactc
     1981 gcccaatatc ggttgagcat ctggagacct gtcataaagg cccgcccact ggcggacaac
     2041 cctgaggttg cctagggggg gcaggatctt cattacagtc tttgccatcc cttcaagaaa
     2101 atccacggat gagtgtatgt tgaagctctt aggctccccc ggcatgccta ttcccatgat
     2161 gaaacttccg tggggtgtct gctgacagta gaaatcatgg tggaaagaga tgaccatcgg
     2221 tcccatcata gcctcaaccg gctccgttat taggatctga tgccgctcgc cccataccgg
     2281 caaatcgatg cctgccattg cggctatctt agcagaatat gccccagcgc agttgacaac
     2341 tgctgacgtt tcgatcctcc ctttatcggt cacaaccgcc tttatgcggt tgttttccac
     2401 ctctatgtcg accacagaag tgtgagtgag aacctctgca ccgagcctct ttgcagcctg
     2461 cgcatatgcg aacgctgtat ggaacggatt ggcgtgcccg tccgtctggc agaacgtagc
     2521 tccgaccata ccctcaaggt taagaaaagg aactatctcc ctcgcgcctt caggcgtgag
     2581 catctccacg tcaatgtcta agctacgctc gagttttaca ttgcgtctga actgctccca
     2641 ctgggaatcc gaataagcga gcatcaaata gccgctctgc ctgaactcaa cacctggctt
     2701 gtaatcgaga tactcgtcca tctgttcgaa gatcctgaca gactcgcgag ccagcacgca
     2761 gttgagttcc gtcccaaact gctgccttac acccgcgccg catctccctg ttgaccctga
     2821 agcaatcgtg ttccgctcta caagcactat gtctttgcaa cccaagcgag caagctcaaa
     2881 ggctgttgcg cacccgacca ccccgccgcc tattacgacg atatcagcgc tctttttact
     2941 catcgccttc gccccttgca attgtggata gtaacacagg cctcgtaggc ggcctgaaag
     3001 tcggcatctc cacttcctcc actgacacgc caagagccgt agcaagctct cgcgcaataa
     3061 gatgcctgca cgttctgccc tggcattggc ccatgcctgc gcgggaaatg cgcttgatct
     3121 catctattgt gtgataacca tttgctatca gacgacgaat atcgcccaag gtaacgtcct
     3181 cgcacctgca tactatcacg tcgtcatcta tccgttccgg tcggtcggtc attcccaaac
     3241 acctccgcac accgtcgcac gacatactcc ggcacagatc ttttcgccag ccggtcccga
     3301 tctgatggct gcaagatccg ccttgagttg t
//
LOCUS       DAOSAI010000292         3327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_18331_length_3327_cov_4.580685, whole genome shotgun sequence.
ACCESSION   DAOSAI010000292 DAOSAI010000000
VERSION     DAOSAI010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18331_length_3327_cov_4.580685"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLM83_09075"
     CDS             <1..408
                     /locus_tag="PLM83_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK71589.1"
                     /translation="LSIMRIWQVSGYYMIMYLTGLQNIPKDLYEAAEIDGATKWQQIK
                     SITIPLLANTTVFATIMLTIEAFNLFEAIYVMTEGRPGGTTNTILYYIYTQAFERYRM
                     GYASAMAWILFAILFVVTILQFNARRKKEEIGY"
     gene            429..1271
                     /locus_tag="PLM83_09080"
     CDS             429..1271
                     /locus_tag="PLM83_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK71590.1"
                     /translation="MKRKKRISTVILYIVLIFLAVIMIYPFVWSVTASFKTNQQIYGG
                     NPLDLIPKPFTLNNYKRVLELLPFGRFLLNSMFLATVVPAISIILASMAAYAFARLEF
                     KGRDFIFMLLLGVMMLPGHVTLIPRYALMIKLGWVNRYIALIIPAIFSASLVFKIFFM
                     RQHFLSIPRELEEAALIDGCSQWGVWWNIMMPNSKPALAALAIIGFKTEWNSFLWPNV
                     VINDFMKMPIQVGLTYLQSNAQSSWDILMAGTTIAIVPVILIFICFQQYFMAGSLTSG
                     MGGR"
     gene            1417..2682
                     /locus_tag="PLM83_09085"
     CDS             1417..2682
                     /locus_tag="PLM83_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOK71591.1"
                     /translation="MKPSFRRALLSAAVVVVMLSMCVPMEAKSNRTKVQVISWWDFTS
                     SEPLKQLKAKFEELNPDLELEYLQVGSGYADKMLVMIAGGADLPDVMMLAMDKIPVYA
                     SKGAIMNLDKYIQKDYKDEMKQLFPVVADALTYNGSFYAMPRDITSKVMFFNTKLFKE
                     AGVAIPDEDWTWADFEAIARKMTRDIDGDGKPDQWGFYFRKYMDGFTHWLMQNGGGLC
                     TKEGKSLIGKPETIEALKFLQKLAVDGVMPTETQARQFGNAQTAPFIAGRVAMVEGGL
                     STTVDLKNAGVDYVIRPLPKGKQRVTTAFVNAWAIPSGAKRPDLSWRVLKFFASKEAQ
                     EIALMSGMGLPARKDTNISAFVKIRPDHKYLIESLSFSKPFPSPVYGVDFFKMVEQEF
                     DLMWLGQRSVEDAVKAVEKQAADILSGKF"
     gene            2789..>3327
                     /locus_tag="PLM83_09090"
     CDS             2789..>3327
                     /locus_tag="PLM83_09090"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOK71592.1"
                     /translation="MLTSEYFANMKAVLERIESTQMEAIEKSAEAITESLLADGAWHI
                     MDTGHMLMYEAIGRTGGLMAVRPVRVSLNVENPVRYRERGVSKKRVFMDSIEGLPQFI
                     VEKSNMLPGDVLLVGSVSGINILPVQMAMDAKKLGLTVIGLTAVEYSSNLEAKHSSGK
                     RLFEVCDIVLDNCSNVGDTL"
BASE COUNT          834 a          803 c          913 g          777 t
ORIGIN      
        1 ttaagcatca tgcgcatttg gcaggtgtcg ggttactaca tgatcatgta tctcacgggc
       61 cttcagaaca tcccaaagga cctctatgaa gcggcagaaa tagacggggc caccaaatgg
      121 cagcagataa agagcatcac gattcctctt ctcgcaaata ccacagtatt cgccaccatc
      181 atgttgacga tcgaagcctt caacctcttt gaagcgatct atgtaatgac tgaagggcgt
      241 cccggcggaa ccaccaacac gatcctctac tacatctata cccaagcatt cgagagatac
      301 aggatgggct atgcgtcggc tatggcgtgg atactctttg ctatcttgtt tgtggtaacg
      361 atccttcagt ttaatgcccg tcgcaaaaaa gaggagatcg gctactaggc cggggagggg
      421 gaaagaacat gaagcgcaag aagagaatct ccactgtgat tttgtatata gtacttatct
      481 tcctggctgt gataatgata tatccctttg tctggtcagt gacagcgtcg tttaagacca
      541 atcagcagat atacggaggg aacccgctcg acctgattcc aaaaccgttc accctcaaca
      601 actacaagag ggttcttgaa ctgcttccgt tcggccggtt ccttctcaac tcgatgtttc
      661 tggctacagt agttcctgcg atctcgataa tcctggcgtc gatggcggca tatgcctttg
      721 cccgcttgga gtttaagggc agggatttca tcttcatgtt gctgctggga gtaatgatgc
      781 ttccgggaca cgtaacgttg attccaagat atgccttgat gattaaattg ggatgggtca
      841 acaggtatat agccctgata atccctgcaa tattcagcgc aagcttggtg tttaagatct
      901 tctttatgcg ccagcatttt ctgagcattc cccgggaact cgaggaggct gccttgattg
      961 atggttgctc gcaatggggc gtctggtgga acattatgat gcccaattcc aaaccggctc
     1021 tggcggccct ggcgatcata ggattcaaga ctgaatggaa cagcttcctc tggcctaatg
     1081 tggtaataaa cgattttatg aaaatgccaa tacaggtcgg cctgacatat ttgcagagca
     1141 atgctcagtc aagctgggat attctcatgg ctggcactac aatagctatt gtccccgtca
     1201 tcctcatatt catctgtttc cagcagtatt tcatggctgg atcgctcaca tccggaatgg
     1261 ggggacgata gggattgtcg aggtttggac aaaggctgtc atggtaatgc atcaacttaa
     1321 gggggcggta gaccggctac gaagcgtcga tgatctgatc actgctgaac cgtgtgagta
     1381 ctacctttcc gcaagcaaaa aggaggaagt gaaaagatga aaccctcttt cagaagggcg
     1441 ttgctttcag cagcagttgt ggtagtcatg ctttcaatgt gtgtacctat ggaagcaaag
     1501 tcgaacagga ccaaggttca ggttatatcc tggtgggact tcacatctag cgagccgctt
     1561 aagcagctca aggcgaagtt cgaagaactc aaccccgacc tggaactcga gtatctacag
     1621 gtaggaagcg gatacgccga taagatgctt gtcatgattg caggcggcgc cgaccttcct
     1681 gacgtcatga tgcttgccat ggataagatc ccagtctacg catccaaggg cgcgatcatg
     1741 aacctcgaca agtacattca gaaggactac aaagacgaga tgaagcagct cttcccggtc
     1801 gtggccgatg cccttacgta taatggcagc ttctatgcca tgccgagaga catcacgagc
     1861 aaggtcatgt tcttcaacac gaagctcttc aaggaagcag gagttgcgat accggatgag
     1921 gactggacgt gggccgactt cgaggctata gctagaaaga tgacccgcga catcgatggc
     1981 gacggaaagc cggatcagtg gggattctac ttcagaaagt acatggatgg gtttacccat
     2041 tggcttatgc agaacggcgg aggcctttgc accaaggaag gcaagtccct catagggaag
     2101 cctgaaacca ttgaggctct gaagttcctg cagaagttgg cggttgacgg cgtgatgccc
     2161 acagaaacac aagctcgcca gttcggaaat gctcagacgg caccgttcat cgcgggccga
     2221 gttgcaatgg ttgaaggcgg actgtctacc acagtcgacc tgaaaaacgc cggggttgat
     2281 tacgtgatca gaccgctgcc caagggcaag cagcgcgtaa ccactgcatt cgtgaatgcc
     2341 tgggccatcc cgtcgggagc gaagaggccg gatctctcct ggcgcgtgct gaagttcttc
     2401 gccagcaagg aagcgcagga gatagccctc atgagcggca tgggccttcc cgcaaggaag
     2461 gatacaaata tcagcgcttt tgtgaagatc cgtcctgatc acaagtacct gatcgagtcc
     2521 ctctccttca gcaaaccgtt cccatcgcca gtgtatggcg tcgacttctt caaaatggtg
     2581 gagcaggaat tcgacctcat gtggttgggt cagaggtctg tggaagatgc cgtgaaggcc
     2641 gttgagaagc aggcggcgga tatcctgtca ggcaagttct agttttgatc caatagatgc
     2701 gtaacggggc gccctgcaac aggtgcgccc tgtcgcgtta ggaatacccg cggcgtttaa
     2761 cctagcaagt atcgtgaggt gcgtgtgtat gcttacatca gagtattttg ccaacatgaa
     2821 ggctgttctt gaacggatcg aatccacgca gatggaggcg atcgagaaaa gtgccgaggc
     2881 cataacagaa tccctcctcg cagatggcgc gtggcatatc atggacacgg ggcacatgct
     2941 catgtacgag gcgattggaa ggacgggcgg ccttatggcg gtccgaccgg tgagagtctc
     3001 tctcaacgtt gaaaaccccg tccgctacag agagcggggg gtctcaaaaa agcgggtctt
     3061 catggactcc atagaagggc tgccccagtt catagtcgag aaaagcaata tgctgcccgg
     3121 cgatgtgctg cttgtagggt cagtctcggg catcaacatc ctgcctgttc agatggctat
     3181 ggatgcgaag aaactggggt tgaccgtaat cgggctcacg gctgtcgaat attccagcaa
     3241 ccttgaggcc aagcattcca gtgggaaaag actatttgaa gtgtgcgaca tcgtgctcga
     3301 caattgttcg aatgtgggcg atactct
//
LOCUS       DAOSAI010000293         3323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_18370_length_3323_cov_2.964504, whole genome shotgun sequence.
ACCESSION   DAOSAI010000293 DAOSAI010000000
VERSION     DAOSAI010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18370_length_3323_cov_2.964504"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLM83_09095"
     CDS             <1..814
                     /locus_tag="PLM83_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOK71593.1"
                     /translation="DVARSMGIVPLIVISNFDGTNFNPDLARDAMLEPTGVATIGAIV
                     DVAVSEGFGGVNIDLENMYPEDRELYTAFMAALSDAAHSRKLTLSNAMAPKWADWPTL
                     PWVGTFDYAALAPSLDFTMLMTYEWGWSGGPPMAIAPVNLVRRVLDYAIGKIPANKIL
                     MGIPLYGYDWTLPAAAGRLADTVTPAEATAIAARHGININFDDIGKAPWFRYIDQSGA
                     EHEVWFEDVRSSVAAHSLVGEYGLRGTSYWNLLFDFPQNWLALEDRYIVRKL"
     gene            1360..3057
                     /locus_tag="PLM83_09100"
     CDS             1360..3057
                     /locus_tag="PLM83_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOK71594.1"
                     /translation="MTQINSVAARVFGVVLAIVIIPVIAVSGASTTSEVAPGTSAVAP
                     SRAEVEAFYSYDAGIPLDVKEIPLGSSPWYREYEISYANLGDTVYSRFMLPREAGASF
                     ETMNPMPCLVGLHGMFSDSAYQFWTIADFCAKRKVAVMTPSLPYHHKRTKGMPLIPGQ
                     QLIVGSPDAIRNNLRRAVIDVRRAVDWLATRDDIDQGAISIAGVSLGGIVASLAFKVE
                     PRFANAVLVVAGSGVRGILENGDTSVLDVFRAATWANMVNPDDFVDALQIVDPINVPD
                     FQPRPALLMNGISDAIMVPDNVTALRRSMARAEQIWTTGGHYFPMYPAEYLLMDYLSR
                     QYAESPSFAQGIRVDVGSGFCFTERVCAPEVGADNLWVDIAVELGSPEYVAAHRVSVP
                     MVNRSVPVIILSRETYLALEPFLMGRHLPAFIYIINGDSPLQLCAAVAYVQALGLGAD
                     PQLYYVSPLEDSGNNPGREGYAVCAASPMDAGRVRNMLESIARAPGSRTSGGFEKANA
                     RIPLTLFGEAKRFQGPTAREDIGLWFAQTLSVSKLKGIPWFPNYPHDDPCWSAGPDR"
BASE COUNT          690 a          870 c          961 g          802 t
ORIGIN      
        1 ggacgtggcc cgttcgatgg gcattgtgcc cctgatagtc atctctaact tcgacgggac
       61 caacttcaac cccgaccttg ccagagacgc catgttggag cctacaggcg tggcaactat
      121 aggggccatc gtggatgtcg ctgtatccga aggttttgga ggcgtcaata tcgaccttga
      181 gaacatgtat cctgaagaca gggaactata cacagcattc atggctgctc tctcagacgc
      241 tgcccactcc cgcaaactca cgctttccaa tgctatggcg ccgaaatggg ctgattggcc
      301 tacgctgccc tgggtgggca catttgacta cgcggcgctg gcgccttcac tcgatttcac
      361 catgctcatg acttatgagt ggggttggtc ggggggaccc cctatggcca ttgctcctgt
      421 taatctggtg cggcgcgtgt tggactacgc gattggcaag atccccgcaa acaaaatact
      481 gatggggata cctttatacg gctatgattg gacgctgccg gcggccgccg gaaggctggc
      541 agatacggtt acgccagccg aggccaccgc aatcgccgcg cgccatggca taaacatcaa
      601 cttcgatgac atcggcaagg ctccatggtt ccgctacatt gatcaatcag gcgcggagca
      661 tgaagtatgg ttcgaagacg ttcgttcgtc tgttgccgcg cattcacttg taggagaata
      721 cggtctgcgg ggcacaagct attggaacct gttgtttgac tttcctcaga actggttggc
      781 actcgaggac cgctacattg tgaggaagtt gtgagagagt tgtgggcggc ctatgccgcc
      841 catcgtacag aggaaatgtc tgatcccgga gctttgcatc accggtatcg ccgtaagagc
      901 tagtcgacta ccaggtcgcg gcccgcggca aagcccatcg cgatctcgtt gccatccgag
      961 atccccagtt ttcgcaggat gtataaggca acactgcagc tggttgacga gtaactcaag
     1021 ccgactcatt gtcatgggag atgacggacc gctgcgctgc taatatctca tcgatgggct
     1081 tgccgttgat ggttgcgaat ttcattgttg attgtgttcc ggtattcctc tcgggcatcg
     1141 aatgcatata tccctcattg aactagtaga gccccttacg ggatatgtga agcggaaccc
     1201 cgagcttgac catctgttct atataggtgt tctgctgtct tgttacttta gcccggccgg
     1261 cgactcgcag tcgtggacaa ttgggaaatc aacaaggagt ggaatgccgc ggcctgctgg
     1321 tgtattatgt atataacttg ctaggaggtt cttacggtta tgacacaaat aaactcagta
     1381 gcagccagag tcttcggagt tgtcctcgct atcgtgataa ttccggtcat agctgtatcg
     1441 ggagcttcta cgacttcaga agtcgctccc gggacgtctg ccgtggctcc atcccgagcc
     1501 gaggtggagg ccttttattc ctatgatgca ggcatccccc ttgatgtcaa agagatcccg
     1561 ttgggatcta gtccctggta tcgggagtat gagatcagct atgctaacct tggagacacc
     1621 gtgtattcca ggttcatgct tccacgggag gcgggggcgt ctttcgaaac gatgaacccg
     1681 atgccctgcc tagtcggcct ccatggcatg ttctctgact cagcgtacca gttttggacc
     1741 attgcggact tctgtgccaa gcgaaaagtt gcagtaatga cccccagcct tccataccac
     1801 cacaagcgca cgaaggggat gcctctcata ccaggacagc agctgatcgt tggttcgcct
     1861 gatgcgatca gaaacaatct gcgtcgagcg gtcatagatg tccgtcgcgc tgtagattgg
     1921 cttgccacgc gtgacgatat cgaccagggc gctatatcga ttgcaggcgt cagtctgggc
     1981 ggaattgttg cctcgttggc cttcaaggtt gagccaaggt tcgcaaatgc cgtgctggta
     2041 gtggctggct caggggttag aggcatactt gagaacgggg atacgagtgt cctcgatgtc
     2101 ttccgcgcag ccacctgggc aaacatggta aaccccgatg atttcgtcga tgcgcttcag
     2161 atcgtcgatc ccatcaatgt gccggatttc cagccccgcc cggcgcttct aatgaacgga
     2221 atttccgatg cgatcatggt ccccgataac gtgacggcgc ttcgccgttc catggctcga
     2281 gctgagcaga tatggaccac gggcgggcac tattttccga tgtacccagc ggaatacttg
     2341 ctcatggact atttgtctag gcagtatgcg gaatctccgt catttgctca aggcattcgt
     2401 gtggatgtag gctctggatt ctgtttcacc gaaagggtgt gcgcaccgga agtgggggcc
     2461 gacaacttgt gggtagacat cgcagttgag cttggcagtc ccgagtacgt ggctgctcac
     2521 cgggtttcgg taccgatggt taacaggtcg gttccggtca taatcctgag cagggagact
     2581 tatttggctc tcgagccttt cttgatggga cggcatcttc ctgcgttcat ctatatcatc
     2641 aacggtgatt ctccactaca gctgtgcgcc gccgtggcat atgtacaggc gttggggctt
     2701 ggcgccgacc cgcagctcta ttacgtctcc cctctggagg attcaggaaa caacccgggc
     2761 cgggaaggat atgccgtctg tgctgcatct cccatggatg cggggagggt ccgcaatatg
     2821 ttggagtcga tcgcacgagc tcctggctct aggacctcgg ggggctttga gaaggcgaat
     2881 gcccgaatcc ccctgacgct ctttggcgaa gcaaagcgtt ttcaaggccc gaccgctcgt
     2941 gaggacatcg ggttgtggtt cgcccagacg ctctcggtga gcaaactcaa gggcatcccc
     3001 tggttcccga attatcccca tgatgaccca tgttggtcgg cggggcccga cagatgactg
     3061 aaaaccggtt cctgcagaag attcacggca gaagcgattt atgcctgtta taggcaaagg
     3121 ctagtcgata cggggagagc tgaagcaggc aattatatgg agcctttgga aggtaagcgt
     3181 caaatagccc ctacctagat atcaagcggg ggccaggata tgattttgtt gctatcttgg
     3241 ggtcgggcag gcgccggata ggctcttgga gtatggcagc agctcatcga gtgcagccgg
     3301 gccgttgatg ttaatgttcg gca
//
LOCUS       DAOSAI010000294         3303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_18521_length_3303_cov_3.977525, whole genome shotgun sequence.
ACCESSION   DAOSAI010000294 DAOSAI010000000
VERSION     DAOSAI010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18521_length_3303_cov_3.977525"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1270
                     /locus_tag="PLM83_09105"
     CDS             95..1270
                     /locus_tag="PLM83_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOK71595.1"
                     /translation="MAQLIDSIRSRAMNIALSRASDPKAVSRRASWLKEGDARFDGRQ
                     PTPFATRHGKDAKPERVICAAIQMKAKTVSSGTEYVELVYRLAAKAAVAGASIVVFPE
                     YSGLPLLGMAPGMRKKLDSASSLDEAVTEAGGPDAGVLDVFKLVGPYAERAYKEAFSS
                     VARELGIYIISGSMVLSDGSTDGSSMRAIGYVFGPQGELVGRYCKSHLMPIEAQWGIS
                     PGNSIPTFNLGGVIVSVPICMDATYFETFRIARFAGADIVAIPSANPEPYNLWYALRG
                     IWPRVQESQVYGIGSSMVGSFAGQDFTGRSSLLAPLELSPGGDGILAQAMTYNEEDVV
                     VAEFDLPLLYKLRAEDPLRFNLALYRKYLPGMYRAAQSSRRAGDAGEARDENKGETC"
     gene            1271..1993
                     /locus_tag="PLM83_09110"
     CDS             1271..1993
                     /locus_tag="PLM83_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOK71596.1"
                     /translation="MRHKATVVCAHPDDLEYFCGGAVSVMIRDDWDICLIIATDGDKG
                     TQDREMSAARLASERRHESMKAAKIVGISKVFFLGKEDGELRSDNSLRPAITRILRSE
                     QPERLITFDPWRKYELHPDHREIGFAALDARLAARLPLYYPEQLRDGLENWTIREVLL
                     FSTDNPDYFVDISDTFDLKLQALRAHKSQWETIWQETRSDLLHEAERMGAMAGCELAE
                     GFKRIYIPEGPVSIKFEHERGD"
     gene            1999..>3303
                     /locus_tag="PLM83_09115"
     CDS             1999..>3303
                     /locus_tag="PLM83_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71597.1"
                     /translation="MKPAAERILTTPSELGDIVSPYVTGNEWVALPDIFPSDASLGSI
                     NIVRMASKGLVEWVGEFEEDQDRRNEPKAPFLRPFVNAIGASGKPAETESVGDIEWTW
                     LGHWIPVGKCRLRTKEGHECVAKIAFCAPVGERGFMVALELSELEARNGASHVQFHLG
                     LEGNWGATVSTVFGSRLMHVTNHAYYSPWTNSLILEARGTSSVGALAFSADSQFDWAI
                     WASKDGVDMKESPVLAVPNGSADIRFRLSSSMTLAANEHRTVAFWIAANADPDGAGTT
                     NVHLRRVGAEAALGNTLSWLEARAHSSRVSPVEIGEADEEILARADRNLHFCRFFAHG
                     KAIDTEELVMLTSRSYRYYVSAVFWPRDTFLWAFPAMLASDHVFAREVLTEGFRRHVR
                     NMGTHAHYIDGVLLYPGFELDQLASYVIALGTYIEATGDASLA"
BASE COUNT          768 a          910 c          937 g          688 t
ORIGIN      
        1 acacttcaat gtagagctgt acgagaacca gctgtcagat gcgtatctcg tcgggcgcac
       61 agtgcggctt aaacagggaa ggaggtagcc gaaaatggcg caactgatcg attcaattcg
      121 cagcagagcc atgaatatcg ccctttcgcg ggcctccgat cccaaagccg tctcgcgacg
      181 ggcttcgtgg ctgaaggaag gagacgcgcg attcgacggt cgtcagccga ccccctttgc
      241 aaccagacat ggcaaggacg ccaagcccga gcgcgttatc tgcgccgcaa tccagatgaa
      301 agcgaaaacg gtctcttcag ggacagagta tgtggagctc gtatacaggc tagcagcgaa
      361 ggctgcggtg gcaggagctt cgatcgtcgt atttcccgaa tacagcggtc tgcccttgct
      421 gggtatggcg cctgggatgc gcaagaagct ggattcagca agttctcttg atgaagccgt
      481 cactgaggct ggaggcccgg atgcaggtgt tcttgatgta ttcaagctcg tagggcccta
      541 tgcggagcgc gcctataagg aggccttctc ttctgtcgct cgcgagcttg ggatctacat
      601 aatctcagga agcatggtcc tttccgatgg ttcaactgat ggcagttcca tgcgggcaat
      661 aggctatgta ttcgggcccc aaggcgaact ggtaggcagg tattgcaagt cgcatctcat
      721 gcccatagaa gcccaatggg gtatcagccc aggaaactcc atccccacat tcaacctagg
      781 cggagtcata gtatccgtcc ccatatgcat ggacgcaacc tattttgaga cattccggat
      841 agccaggttc gccggagccg acatcgtcgc gataccttcc gccaaccccg aaccatacaa
      901 cctatggtac gcgttgcgag gcatatggcc tcgagtacag gaatcccagg tctacggcat
      961 cggttcatct atggtgggat cctttgcagg ccaagatttc accgggcgaa gttctctctt
     1021 ggcgccgttg gagctttcgc cgggaggaga cggcatcttg gcgcaagcca tgacgtacaa
     1081 cgaggaggac gtggtagttg cagagttcga cctgccgctc ttgtacaagc ttcgggcgga
     1141 agatccgctg cgtttcaatc ttgccctgta ccgcaagtat ctgcctggta tgtaccgcgc
     1201 agcccagtct tcacggaggg ctggggatgc aggtgaagct cgggacgaaa acaaaggaga
     1261 gacgtgctaa atgagacata aagctacggt agtgtgtgct cacccagacg atctcgaata
     1321 tttctgcggc ggagctgtct cagtcatgat tcgtgacgat tgggacatat gtctcataat
     1381 cgccaccgat ggcgacaagg gcacacaaga cagggagatg tccgcagccc ggcttgcctc
     1441 cgaacgccgc cacgaatcta tgaaagccgc caagatcgta ggtatcagca aggtcttctt
     1501 cctaggcaag gaagacggag agctccgctc cgacaactcc cttcgacccg ccatcacgcg
     1561 catcctcagg agtgaacagc cggaacgcct gataacattc gatccctggc gcaaatatga
     1621 actccaccct gaccatagag aaattggatt cgcggcgctc gatgcgcgcc tggcagcaag
     1681 gcttcccctg tattatccag agcaacttcg agacgggctc gaaaactgga ctatcagaga
     1741 agtcctgctg ttcagcaccg acaaccctga ttattttgta gatataagcg acacattcga
     1801 tctcaaattg caggcactcc gtgcccataa aagccaatgg gaaaccatat ggcaggaaac
     1861 ccgctctgat ttgctgcacg aggcagagag gatgggagcc atggccgggt gcgaacttgc
     1921 tgagggattc aaacggatat acatcccaga aggcccggtc agcatcaagt tcgaacatga
     1981 gcgaggagat tgatccatat gaagcctgca gctgaacgca tactcactac tccatccgag
     2041 cttggcgaca tagtctcccc atacgtcact ggcaacgagt gggttgcact gcccgacatc
     2101 tttccctccg atgcaagttt ggggagcata aacatagtgc gtatggcctc aaaagggctg
     2161 gttgagtggg tgggtgagtt tgaggaggat caagaccgcc gcaacgagcc gaaggcgccg
     2221 ttcctcagac cgtttgtgaa tgcaattgga gcctcgggaa agccggccga aaccgaatcc
     2281 gtcggcgaca ttgaatggac ctggttaggc cactggatcc ccgtgggcaa gtgccgcctt
     2341 cgcacaaagg aaggacatga gtgtgttgcg aaaatcgcct tttgcgcccc tgtaggcgaa
     2401 cggggcttca tggtggcctt agagctctca gagcttgagg cacgcaacgg cgcaagccat
     2461 gtccagtttc acctagggct cgaggggaac tggggcgcca ccgtcagcac ggtgtttggg
     2521 agcaggctca tgcatgtcac aaaccacgcc tattattcac catggaccaa ttcactcatc
     2581 ctcgaagcca ggggaacttc gagcgtcggt gcattggcat tctcagccga ctcccagttc
     2641 gactgggcta tctgggcaag caaagatggc gtcgacatga aggaaagccc ggtcctggcc
     2701 gtgccgaacg gctccgccga tatccgcttt cgtctctcct caagcatgac cctggccgca
     2761 aacgagcatc gcacagttgc attctggatc gctgccaacg ccgatcctga cggggcggga
     2821 accacaaatg ttcatcttcg gcgagttggc gccgaggcgg cattgggaaa cacgctctca
     2881 tggcttgagg ccagagcaca ttcatccagg gtttcgcctg ttgagatagg ggaagcagac
     2941 gaggagattc tcgcgcgggc ggatcgcaac ctacattttt gcaggttttt tgcacacgga
     3001 aaggctatcg acaccgaaga gttagtcatg cttacatcca ggagctatcg ctattacgtg
     3061 tctgcggttt tctggccgag agacacattt ctctgggcat tcccggcgat gctcgcctcc
     3121 gatcacgtat tcgcccgcga agtgctgacc gaaggatttc gccgccacgt gcggaacatg
     3181 ggcacgcatg cccactacat agacggcgtc ctgctttatc caggctttga acttgatcag
     3241 ctggcatcat acgtaatcgc ccttggaacc tatatcgaag cgacaggcga tgcatcgctt
     3301 gca
//
LOCUS       DAOSAI010000295         3297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_18562_length_3297_cov_4.216225, whole genome shotgun sequence.
ACCESSION   DAOSAI010000295 DAOSAI010000000
VERSION     DAOSAI010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18562_length_3297_cov_4.216225"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(442..1635)
                     /locus_tag="PLM83_09120"
     CDS             complement(442..1635)
                     /locus_tag="PLM83_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOK71598.1"
                     /translation="MKMKTSRAGTFVPQPGGYTAFVPDPLPPDPPIQLNDFELLELLS
                     RADRALGRLDGATEILPNPDLFVDMYVRQEAILSSQIEGTQASLIDVLEYELEGDSKR
                     FPGDTQEVANYVRAMNYGLARLEDIPLCLRLIREIHHLLLDGTRGCERDPGQFRKTQN
                     WIGPPGVGIRDAIFVPPPVPEMLKALDDLERFINSDDHMPILVKCALAHAQFETIHPF
                     LDGNGRLGRLLITFMLCERQVLRRPLLYLSLFLKRNRSEYYDKLNAVRTHGDFEGWIK
                     FFLRGVAEISLEATETARAILELREEHRTLLTANGFATANHMNLLEHLYEYPIISVKK
                     AVDLLDVAFATANDTIARFVKLGLLQEITGKQRGRLFAYVPYLQIFTEAGVRLDRADS
                     ALASD"
     gene            2030..2485
                     /locus_tag="PLM83_09125"
     CDS             2030..2485
                     /locus_tag="PLM83_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71599.1"
                     /translation="MAQTDSIRQLFFEKGLGYAEIVRTTGFEVKTVKKYVHMENFNQP
                     LPKPRQIRGSKLDKQREQIDEWMVADKQERKKQRHTAKRTFDRIAEKHPGFDYSYRLV
                     ADYVSQKKKVRIPMKSVHSFWIKESSDSGEPRLTKSERSDAWRKVMSLG"
BASE COUNT          810 a          820 c          858 g          809 t
ORIGIN      
        1 ttgcgactat acacctatcg caacacgagt acaagtcaat tagtggcaaa ggctgctcgg
       61 caacccttct gataattcac tcgatctcca tctgggggtg ccgaagatcg gatgggttct
      121 ggctggcttg caaagacaat cgctcctcgt aatatctcag agggtcagca tcagatggca
      181 cctgccgtat ttcgacctgt cgaaacgacc cggcctatag agcacaattt gacaattgca
      241 ggggggtagt actcttttga ctgatgaggc catcaaggtc atccatgaag ctgcacaggg
      301 cgtggcgtgg accgttgcgt aattgtgacg gacgataatc atggggtgca gacatgcctg
      361 taattataga atcaggctcc attctgaaat aaggacgtgc ggttggggtg catgcctggc
      421 tgtaaatggc atgaccatga tctagtcgga ggccaaagcc gagtctgccc ggtcaagtct
      481 tacgccggct tcagtgaaga tctgcaagta tggaacgtac gcaaaaagcc gaccccgttg
      541 ctttccggtg atctcttgca gcaaccccag tttcacgaac cgcgctatag tgtcgttggc
      601 ggtggcaaat gctacatcaa gcaagtcaac ggcttttttc actgagataa tgggatattc
      661 atagagatgt tcaagcaggt tcatgtgatt ggcggtggca aacccgttcg ccgttagaag
      721 cgttctgtgc tcctccctga gctccagaat tgcacgagcg gtctccgtag cctcaagcga
      781 tatctctgca acacccctca agaagaactt gatccagcct tcaaagtcac cgtgggtacg
      841 cacggcattg agcttgtcat agtattcact gcggtttcgc ttaagaaaca acgacaagta
      901 gagcaaaggt cttctcagca cttgtctttc gcatagcatg aaggtgatga gaagcctgcc
      961 aaggcgaccg ttgccatcaa ggaacggatg gatggtctca aattgtgcat gggccagtgc
     1021 acacttgacc aagattggca tgtgatcgtc tgagttaata aatctctcca gatcgtccaa
     1081 tgctttgagc atctccggta cgggtggagg cacgaatata gcgtctctga tcccaacgcc
     1141 gggaggtcca atccagtttt gagtcttcct aaactggcct ggatcccttt cgcagccacg
     1201 cgttccatca agaagcaagt gatggatctc tcgtatgagc cgcaaacaaa gggggatgtc
     1261 ttcaagacgc gccagaccat aattcatagc ccttacgtaa ttcgccactt cctgagtatc
     1321 gccggggaac cttttggagt ctccctccag ctcatactcg agcacgtcta tcagcgaagc
     1381 ttgggtgcct tcgatttgcg aggagagaat cgcctcctgc cggacataca tgtcgacaaa
     1441 taggtctgga ttcgggagta tctcagttgc accgtccaat ctgccaagtg ctctatctgc
     1501 tcttgatagg agctcgagaa gctcaaagtc attgagctga atcggagggt ctggaggcaa
     1561 cggatcgggg acgaaggccg tgtaccctcc gggttgagga acaaacgttc ctgctcttga
     1621 tgttttcatc ttcatgcccc tcctctaatt agtgtcgggc ttaattaccc caccttaggg
     1681 cagccttaat tttagtctag ccgcattctc taaaataagc aacaatgaaa ggctccgcta
     1741 tgccttcccc agaaggagag cgaattgcct ttcggccctg aggaacatga ggctatactt
     1801 gccgcgcgaa tgcactgatg acccggagcg ttgccgcagg gtgcatgtgc cagattgatg
     1861 tctcattcaa gaccaagcca gacattgcga ttgatctggt tgatgaggcc gacgcgaacg
     1921 gggttccata cgaaaccatt acgttaggat gtagaggctg tgacccaaga cccccaaaaa
     1981 ccaaatagca gtccgcagtc cctttgtgca gacgaagaag gccattgcag tggcacagac
     2041 agattctata agacaactgt tcttcgagaa aggcctgggt tatgctgaga tcgtaaggac
     2101 tacgggtttc gaagtgaaga ctgtgaagaa atacgtacat atggagaatt tcaatcaacc
     2161 tcttccgaag ccgaggcaga ttcgcggctc taagctggac aagcagagag agcaaatcga
     2221 cgaatggatg gtggcggata agcaggagcg aaagaagcag cgccatacag caaaaagaac
     2281 tttcgaccgg attgcagaga agcatcccgg gtttgattat tcgtaccgcc tggtggcaga
     2341 ttatgtctcc caaaagaaga aggtgcgtat tccgatgaaa tccgtccact cgttctggat
     2401 caaagaatcc agtgattccg gagagccccg tctaaccaaa tcggagcgaa gcgacgcgtg
     2461 gcgaaaagtc atgtctttag gttagtgatt cgctatgatc tctgttccgc cgatatgtcc
     2521 attcctggcc gaccagcagg ttgaagcgcc catcgaagga taactcatct atgttgggcc
     2581 ttttgttttg ggataagtat gcttcgctca tcccagtcag gcgcatgtcg tttagccgtg
     2641 tcatgatatg ttcaactaac atcttgttaa gcctcctacc taaagtagcc tgggccgctg
     2701 ggtaggcttt cgccagcctc ggccaaaggc cctctgatgg attgccactc tatggtcgcg
     2761 acctgatacc tccacagtcc ttgtagtcag tcgcacatcg tatgaggcta ttggggatgt
     2821 tgtacaggtt gtggtcgact gcaatgtgat agtttatgtt tgcagttgcc ttcctctatc
     2881 tagcgaacac gtaatgcgtc tcgggaagag gtttgagcag agacctgcgc gttccgtcca
     2941 tcttctgaaa aggtttgttg ttcagcacat ccagattctc ggggataggg ctgttcagct
     3001 ggtgaggcca aagaaggtga tgttgcgtat caccccaagc cttcgatacc ctagctttga
     3061 aattgtgggg acccatatct ctgccgatac cgcaaaaaac tccagaggat tgcaggtcaa
     3121 gccaactcat gatctcagac tctgcgtcat cctaggccct actatatgtg tagttgctcg
     3181 ctcctaacac tgcaacgtac acatatgcgt cctcgatttg gatccactat cggcacggtc
     3241 ttgagtcacg tccgatataa tgctgtaaaa aggccttagc cctgttgtgg tgggtgg
//
LOCUS       DAOSAI010000296         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_18607_length_3292_cov_4.886314, whole genome shotgun sequence.
ACCESSION   DAOSAI010000296 DAOSAI010000000
VERSION     DAOSAI010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18607_length_3292_cov_4.886314"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(492..1052)
                     /locus_tag="PLM83_09130"
     CDS             complement(492..1052)
                     /locus_tag="PLM83_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71600.1"
                     /translation="MDLRQPSARQRQIVTTWPVRVESEIAKSIRNDRWNVPYLGEWLM
                     TCWLDYLVVSADYLDHSRPEWLRQTSTGHKMEFDREYKGLRTAFEFQGQQHFQTTLMY
                     PDSLALVEQQRRDNTKAGICARHGVRLVEVTAEDLNLRVMLQKVAWLPLRQYRQRGPI
                     VSELTQIYDSYMGHLAKTQRQSESCD"
     gene            1525..1755
                     /locus_tag="PLM83_09135"
     CDS             1525..1755
                     /locus_tag="PLM83_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71601.1"
                     /translation="MNRTGSETPIRIEVEATGGTSVAAGDRVRCGQDLGISPDFSERV
                     VCHADGLVEACVFNPDTHRFEITIVPDNAKKE"
     gene            1822..2124
                     /locus_tag="PLM83_09140"
     CDS             1822..2124
                     /locus_tag="PLM83_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOK71602.1"
                     /translation="MVRHVVMWSLADEAAGGVKQQNAAIIKEGLEALVGKIEGLIGLE
                     VGINALELAGNYDIVLIADFQDEAALERYQVHPEHVKVADFIKKVRLERVAVDYLM"
     gene            complement(2164..2853)
                     /locus_tag="PLM83_09145"
     CDS             complement(2164..2853)
                     /locus_tag="PLM83_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOK71603.1"
                     /translation="MKKLVVLTGAGISAESGIRTFRDSDGLWYEYDVMDVATPEGWRR
                     DPKLVLDFYNQRRRELYRTKPNAAHLALARADEDFDVHIITQNIDDLHERAGSAKVLH
                     LHGELKKSRSTADPSLIYDIEGTELDWGDKCELGSQLRPHVVWFGEPVPAMAEAAEIA
                     QSADIFAVVGSSLVVYPAAALVQFVPSDAPVFLVDPKEVALSSLREVTHIMEKATTGV
                     PKMLESMKKLG"
BASE COUNT          774 a          840 c          868 g          810 t
ORIGIN      
        1 tacagcgaat ggttcgtagt gttttttgct acactacgaa tatttcgtgg atacggttac
       61 atttataggt aatgtatgga aagtaatgtg tattggggta tgctgatgtc gaattgacct
      121 cgtttcgggg tcggacggtt gccatattag cccaataaga tacagagtat tccatatagc
      181 acccagataa accaaggtgc tctcacgtta tgttaactta tattttgacc ctacgatttt
      241 ttcgtagcat aagaaaaaac attacgagaa aatcgtaggg tcagtggtga tcgacccacg
      301 tctgatgagc cgttcatcaa ttcgttgcat gattggagtc tgcctctata actgtaagcg
      361 tcaaatagcc attccccgac ttcccagacc ctagatgcgg atatgttcgt ctgtagacta
      421 tggggttagc ttcacacgaa ctcacacgaa caatgaagcc gaggaccgca ggctttatcc
      481 ccggcgcact ctcagtcgca gctttcactc tgcctttgag ttttggcgag atgacccatg
      541 tagctatcgt agatctgagt aagttcgctt actattggcc ctcgctgtct atattgccgc
      601 agtggcagcc acgcaacctt ttggagcatt actcgaaggt ttaggtcttc tgcggttacc
      661 tctacaagcc ggacaccgtg ccgtgcacaa atccctgcct tcgtattgtc gcggcgttgc
      721 tgttcaacca gtgccaagga atccggatac atcagggtcg tctggaagtg ctgctggcct
      781 tgaaactcga aagctgttct caacccctta tactcacgat caaactccat tttgtgccct
      841 gttgatgtct gccgaagcca ttcaggcctc gagtggtcga ggtagtcagc tgacacgaca
      901 agatagtcga gccaacaagt catcagccat tcgccaagat acgggacatt ccacctgtcg
      961 ttccgtattg acttggcaat ctccgattcc accctaaccg gccaggtggt gacgatttgc
     1021 ctctggcgcg cgctaggttg ccttagatcc ataacccatc ctttggattt tagatgtgca
     1081 accgctgctc gtgccgagtc cagtgatgag ccaatgcctg cagcgatgtt gctgattgat
     1141 tccgggcgcc ttccaatcag gaacaggtag acactctgag cccgtggaca ggctcgagta
     1201 tcattgaaca gagcctctgg gattgtgtgc tgaatgtgct gtcggaagga caacgcattt
     1261 cacctcacga aaaagcaggt caagaacgta ttgatattat ccacataata ccatccacgg
     1321 gtcgtgcagg caatatggtc agacgtttgt catcctggtt agcctgaaag aacatctcgt
     1381 tgcgggcaga tacttcgaga agggcctaag gtgaaggcct ttagcactga atggagaaat
     1441 caagcttgca gactatcatg tagttggctt gtcaaaaaac tctgcgggtc ttgaaaaccg
     1501 ggcgaaaaag gagtggctga aactgtgaat aggacgggat cagagacgcc aataaggatc
     1561 gaagttgagg cgacgggtgg aacctctgtt gcagcgggcg acagggtgcg ctgtggtcag
     1621 gacttgggca tttcccccga ctttagtgaa cgtgttgtgt gtcatgccga tggtttggta
     1681 gaagcttgtg tcttcaaccc tgatacgcac aggtttgaga taacaatcgt tcccgataat
     1741 gcaaaaaagg agtagagtag acttgcatcc gcaatacggt ttgatgagaa ctggctgagc
     1801 ggccgaagga ggactagaaa agtggtaagg catgtagtca tgtggagcct cgcagatgag
     1861 gctgctggcg gcgttaagca gcaaaacgca gccattatca aggaggggct tgaagccctt
     1921 gtcgggaaga ttgaaggctt aatcggcctg gaagtaggca tcaatgccct cgaacttgct
     1981 ggaaactacg acatagtgct catagctgat ttccaggacg aagctgcttt ggagcgttat
     2041 caggtccatc ccgagcacgt gaaagtagcc gacttcataa agaaggtcag gctggagcgg
     2101 gttgccgtgg attacttaat gtagcatcca taacagatac agatacccga cagatttccg
     2161 gcctcagccc agcttcttca tgctctccag catcttcggc acacctgtag ttgctttttc
     2221 catgatgtga gtgacttcgc gcaacgatga aagagcaacc tccttgggat ctaccagaaa
     2281 cacaggagcg tcagaaggaa cgaactgaac cagtgctgcg gcggggtaga ccacgaggga
     2341 actgcctact acggcgaaaa tgtcggcgct ctgggcgatc tccgccgctt cggccattgc
     2401 cggcacaggc tccccgaacc agaccacgtg cggcctgagt tgagatccta gttcgcattt
     2461 gtctccccag tctagttcgg tgccttctat gtcgtagatc agtgatgggt cagctgtgga
     2521 acgcgatttc ttcagttcgc cgtggagatg gagcaccttg gcgctgcctg cccgctcgtg
     2581 gaggtcgtcg atgttctggg tgattatatg tacgtcgaag tcctcatcgg ctctagcaag
     2641 cgccaaatgg gcagcgttgg gctttgtgcg gtagagctcc cttctccgct ggttgtagaa
     2701 gtcgaggacc agtttgggat cacgacgcca tccctccggg gtcgctacat ccatgacgtc
     2761 gtattcatac cagagaccgt ctgaatctcg gaaagtcctt attccgctct cagcactgat
     2821 accagcgcca gtcagaacca caagcttttt catgcgtcga atatcattcc cttcctccaa
     2881 gccaaacacc gtgtttatct caatgtctgc cttcctgggt gtgccaacga agtggcccgg
     2941 tggctggctc gagtttccgt tgaaggcttc cactactcat tataccagta gcccccaaga
     3001 cgggcagatt gagcggcgtt gtcccaccac atggttggtg atcttcccag aggtcgtgct
     3061 gaggcgattg gcccgtttgc ctgattggac tggggctatc gcctggccga aatgacgaga
     3121 caccgctacc tataggcagt atctgcttca agccggttca tgtgtatctt gcacgcagca
     3181 acccaagctc gaccgccctc cacccctact tgggccacca actgcaaaca gggatagcgc
     3241 atacgcgccg gaacgctgaa ggtataccca tcccccatga cgaatgctac ct
//
LOCUS       DAOSAI010000297         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_18802_length_3265_cov_4.571340, whole genome shotgun sequence.
ACCESSION   DAOSAI010000297 DAOSAI010000000
VERSION     DAOSAI010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18802_length_3265_cov_4.571340"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1386)
                     /gene="hisD"
                     /locus_tag="PLM83_09150"
     CDS             complement(88..1386)
                     /gene="hisD"
                     /locus_tag="PLM83_09150"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOK71604.1"
                     /translation="MNTPIGIKTIAACDYLQQRIRRGPEDFEEVEQKVRPIIEDVRRR
                     GDQAVLDYTAQFDGADFVPESIETTSADIDHAYSVVDQGVVEALRKAADNIRCFHERQ
                     VQQTWACILDDGAIMGQAIRPIERVGIYAPGGAAAYPSSVLMCAVPARIAGVQSVIVC
                     TPPGPGGVANPYVLVAADIVGVDKVFKVGGCQAIAAMAFGTESVCAVDKIVGPGNAYV
                     TAAKRLVFGYVDIDMLAGPSELVVLADMSADPRFVAADMLSQAEHGPDSEAILVTTRC
                     EVAASVVREIAVQVESLSRRDTIIRSLSEHGLIIIADDLDQAVDVVNRYAPEHLEVMV
                     DDPHALLSRLRNAGSILLGPLTPVAATDYAAGPNHVLPTGGTARSGSPLSVRDFVKIQ
                     NITSLTRVALDDMMDAAETIAAVEGLDAHAAALGARRVGL"
     gene            complement(1485..2234)
                     /gene="hisG"
                     /locus_tag="PLM83_09155"
     CDS             complement(1485..2234)
                     /gene="hisG"
                     /locus_tag="PLM83_09155"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOK71605.1"
                     /translation="MEQVSVAQRTPVRESEVASLNLGNCLSIALPKGRLLQPSLDALA
                     EAGLQVPSMDEIDRRLIYTTGDGSMRFILARPADVPAYVEHGAADLGITGKDALLEGD
                     WGVCELVDLGFGRCRLVVAALQGAIAPDGCGSEGAVCKLPLPRSGHTLRVATKFPAIA
                     RRHFALKRDLCVEIIKLNGAVEIAPIAGLSDVIVDIVDTGRTLADNGLVPIEEVAQLS
                     ARLIANRASLKLKSTVIHQIASALCDRSRSI"
     gene            complement(2174..3253)
                     /locus_tag="PLM83_09160"
     CDS             complement(2174..3253)
                     /locus_tag="PLM83_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOK71606.1"
                     /translation="MDSFPTWGRYYLPADALIRRHIESSLAWTFEARGYEEIVPPLLV
                     GLDMAVLDSSGSTFTNGLAGAEKPYAVLARDGSFFALRSDMTVPVAALAVRHLLPSES
                     PVRLYYSGSVFRPQKAGNGKPEERRQFGAELIGTDAADSVKADCEILAMAAEGLINTG
                     VRDFCIGLSDAKLFLAMLDAARVEDDAKDAIWSALASRDYVRYGELVSSLDDGGDPQK
                     ALGIDLLKRLLALESTEESLDALRRAGVCDAASELIRILDVLEERGLGKCFRLDLSIV
                     REFRYYTGVVFEGYAVGCANAVIGGGRYDKLLARFGSSAPAAGLAVDVDAVVSALQAV
                     EGGERRWNRYLWPNARPCAKVRSRH"
BASE COUNT          706 a         1033 c          860 g          666 t
ORIGIN      
        1 gaagcccttg aagttcaggc ctggcgaact cggccaccga acccatatcc cgttcattgg
       61 cgcctcctct cccggcaact gaggctatca taatcctacc ctccttgccc caagggcagc
      121 tgcgtgagca tccagccctt cgactgctgc gatggtctcg gcggcatcca tcatatcgtc
      181 tagtgcaacc cgcgtaagcg atgtgatgtt ctgtatcttc acaaaatccc gaactgaaag
      241 aggcgatcca ctgcgagctg tgccacctgt gggcaataca tggttcggtc cggcggcgta
      301 gtcggtggcc gccaccggcg tgagcggacc tagcagtatg ctgccggcgt tgcgcagccg
      361 actgagaagg gcatggggat cgtccaccat cacttcaagg tgttcagggg catagcggtt
      421 taccacgtct acagcctggt cgagatcgtc ggcgattatg ataaggccgt gttcgcttaa
      481 agaacgtata atcgtatcgc gacgagacag ggactcaacc tgaacggcta tttcgcggac
      541 aacactcgcg gcaacctcgc atctggttgt caccaatatg gcttccgagt caggaccgtg
      601 ctcagcctga gaaagcatgt ccgccgccac gaagcgcggg tcggcggaca tgtctgctag
      661 cacgacaagt tcgctcggtc cggcgagcat atcgatgtcc acatagccaa agaccaacct
      721 tttggctgca gtcacataag cattgcccgg tcccacgatt ttgtctactg cacagacgga
      781 ctcggtgcca aaagccatcg ccgcgatggc ctgacatccc ccaaccttga acaccttgtc
      841 gacgccaacg atgtcggccg ccacgagcac ataggggttg gcaacgccac cgggaccagg
      901 tggagtgcag acaatcacgc tctgcacccc ggctatccgg gcaggtaccg cgcacatgag
      961 cacagaggac gggtaggccg cggcgccgcc aggcgcgtag atgccaaccc gttctattgg
     1021 tcggattgcc tgccccatga tggctccatc atccagaatg cacgcccaag tctgctgtac
     1081 ctgccgctcg tggaagcacc gaatattgtc ggccgccttc cggagagcct ctacaacgcc
     1141 ctgatccacc acagagtagg cgtgatcaat gtcggcgctc gtggtctcaa tgctttctgg
     1201 cacaaaatct gcaccgtcga actgcgcggt gtagtcgagc acagcctggt caccgcgtct
     1261 tcggacgtct tcaatgatgg ggcgaacctt ctgctcaacc tcttcgaagt cctcaggccc
     1321 tcgacggatt cgttgctgca agtagtcgca agctgcaata gtctttatcc cgattggcgt
     1381 attcatcttc gcctcaacac ctcctggcat tggcatgttt taatggccat aaacctcgcc
     1441 tcagctttac agcaatccat ctgtctgctc gatgcctaag tccgtcagat gctgcggctt
     1501 cgatcacaaa gcgccgacgc aatctgatgg atgactgtag atttcagctt gaggctcgcg
     1561 cgattcgcaa tgagcctcgc tgataactgc gctacctcct ctattggcac aagcccgttg
     1621 tcggccaatg tacggccggt gtccactatg tccacgatta cgtcggaaag ccccgcaata
     1681 ggagcaatct ccacagcccc attgagcttg atgatctcga cgcatagatc ccgtttcagc
     1741 gcaaagtggc gtctggcaat ggccggaaac ttggtggcta cccgtagagt gtggccacta
     1801 cgcggcaaag gcagcttgca gacggccccc tcagagccgc agccatctgg ggcaatcgcc
     1861 ccctgaagcg ctgcaacaac caagcggcat ctcccaaaac ctaagtcgac gagttcgcaa
     1921 acgccccaat cgccttcgag caacgcatcc tttccggtta tccctaggtc ggcagcgcca
     1981 tgttcgacat atgctggcac atccgcaggg cgggcaagaa tgaaccgcat actgccgtca
     2041 cccgtcgtgt agatgagtct tctgtctatc tcatccatgc ttggaacttg aagtcccgcc
     2101 tccgccaggg catccagcga tggctggagc aatcgacctt tgggaagcgc gatgctgaga
     2161 cagttcccta gatttagtga cgcgacctca ctttcgcgca cgggcgtgcg ttgggccaca
     2221 gatacctgtt ccatcgacgc tcacctcctt ctactgcttg caaagccgac accacggcgt
     2281 ccacatccac agccaaaccc gctgcgggcg cagatgaccc aaatcgcgcc agcagtttgt
     2341 cgtaccgtcc accgccgatc acggcatttg cgcacccaac ggcatatccc tcgaacacga
     2401 ctccggtgta gtaccgaaat tcccggacta tggacaggtc gagacgaaag cacttgccca
     2461 aacccctctc ttccaagaca tcaagtatcc tgataagctc ggaagctgca tcgcagaccc
     2521 ctgccctcct gagcgcatcg agcgactcct ctgttgactc tagggcaagc aagcgcttga
     2581 gaagatcgat tccgagggct ttctgaggat cgccgccgtc atcgagggac gatacgagct
     2641 cgccatagcg gacatagtct cgggatgcca gcgctgacca gatggcatct ttcgcatcat
     2701 cctcgacccg tgctgcatcg agcatggcca agaagagctt agcgtcgctc aggccgatgc
     2761 aaaagtcccg cactcctgtg ttgattagcc cttcggcagc catggcaaga atctcacaat
     2821 cggctttgac gctgtctgca gcgtctgtcc cgatcagctc agcgccaaac tggcgccgct
     2881 cctccggttt tccatttccc gccttctggg ggcgaaagac gctgcctgag taatagaggc
     2941 ggacaggact ttccgatggc aagaggtggc gcactgccag agctgcaaca gggacagtca
     3001 tgtcagaccg cagggcaaag aagctgccat cccttgcaag gactgcgtat ggcttctctg
     3061 ccccagccag gccattggta aaggtcgaac cactcgagtc gagtacagcc atatccaacc
     3121 ccaccagcag cggcgggact atctcttcgt agccccgggc ttcgaacgtc cacgccagac
     3181 tgctctcaat atgtcgtctg atgagggcat cagcggggag atagtatctt ccccaagtcg
     3241 gaaagctgtc cacaatcgat cacgc
//
LOCUS       DAOSAI010000298         3256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_18873_length_3256_cov_4.095908, whole genome shotgun sequence.
ACCESSION   DAOSAI010000298 DAOSAI010000000
VERSION     DAOSAI010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18873_length_3256_cov_4.095908"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLM83_09165"
     CDS             complement(<1..224)
                     /locus_tag="PLM83_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71607.1"
                     /translation="MDVRRDNGCVCRVREFYESNVEMEWVRLARHRTEFALTARAMEE
                     FLQPASTVLDCGSGPGRYSLLLASRGHKVTL"
     gene            complement(284..1102)
                     /locus_tag="PLM83_09170"
     CDS             complement(284..1102)
                     /locus_tag="PLM83_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOK71608.1"
                     /translation="MNIGVSTLLYENEDIITAVSSIAEAGQKRIELFCEIAGFRPGEV
                     SDETVDTLADLALEHGITYSIHPPCIGLNPASSDSSERARVVEAYIGTIELATRLGIR
                     DMVVHCGSKSDPRVPTEIALGFASQTLQTVGDVAQQAGVVMMLENTCWKETSILESPQ
                     DLIDLADLCPPSTRLLLDVGHAMIWGVNPTECARAWFPRLAQIHAHDNHGECDEHLPL
                     GDGIIDWKELISFLAMERWDGVFMIEVGQQEDSADALERSLDIVRECLAQCANA"
     gene            2053..2460
                     /locus_tag="PLM83_09175"
     CDS             2053..2460
                     /locus_tag="PLM83_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71609.1"
                     /translation="MKQLLPWIARSGTGEQLHVKRGASQRDDIGCIANIALDYGIDLA
                     FDWSSPLILTPFVEWTEPSIDNALSSVMRLLTLSEVARDEAISCKLVEIQRSLHAAKS
                     SLRLRDRLSPQEKGLFDQRLRERVSRLNVMLRG"
     gene            2467..>2845
                     /locus_tag="PLM83_09180"
     CDS             2467..>2845
                     /locus_tag="PLM83_09180"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOK71610.1"
                     /translation="MTNRKDLLDMPVMTIDEGAEAGRITGLVIDPISVHAVALIVSTN
                     ERFNAPKAVPFPLVQGLGEDAVMIENKSAMAEFSSLPELTTIGKTDSELCGLTVIART
                     GKIIGYVEDFAVDDETGRIESFLV"
     assembly_gap    2846..2855
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2972..>3256
                     /locus_tag="PLM83_09185"
     CDS             2972..>3256
                     /locus_tag="PLM83_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71611.1"
                     /translation="MRMPTHPVARRLAMAAIAVVSIVVQDATAVVRLPMTTNKSPECI
                     NPLAHTCHVLPCLDSKARFENSEGARWRPEKKGLSGIVAGSGMLSVRDRTS"
BASE COUNT          800 a          907 c          781 g          758 t
ORIGIN      
        1 agcgtaacct tgtgtccgcg agaggcaagc aagagagagt accggccggg accgcttccg
       61 cagtccagga cggtagatgc cggctgcaag aattcctcca tggcccgcgc agtcagcgca
      121 aattccgtcc tatgtcttgc aagccgcacc cattccatct cgacgttgga ttcgtaaaac
      181 tccctcaccc tgcatacgca gccattatcg cgtctcacat ccacgcagtt tccctcccca
      241 ccttttgtgc cagcagaggc tgcaggccga ctctatcacc tactcacgca ttggcacact
      301 gggccaagca ttcgcgcact atatcgagag aacgctcgag cgcatcagca gagtcctcct
      361 gctgtcccac ttctatcatg aataccccat cccatctctc cattgcaagg aaggatatga
      421 gttctttcca gtctatgatg ccatctccca gcggcaggtg ttcatcacac tcgccatggt
      481 tgtcatgggc atggatctgc gctaacctag gaaaccaagc gcgggcacat tcagtaggat
      541 tcactcccca gatcatcgca tgccccacat caagaagcag ccgcgtggaa ggcggacaca
      601 agtcagccag atcgatgaga tcttgggggc tctccagaat gctagtctct ttccaacacg
      661 tattttcgag catcatcact accccggcct gttgtgcaac gtcccccacg gtctgcaatg
      721 tctgactcgc aaagcccagg gcgatctccg tcggcaccct agggtcggac ttgctcccac
      781 agtggaccac catatcgcga atgccgagcc tggtagcgag ttcaatagta ccgatatagg
      841 cttcaactac ccttgcccgt tcggacgaat cagatgaggc agggttcaga cctatacaag
      901 gcggatgaat ggagtacgta attccatgtt ctaaagcgag gtccgcaaga gtgtccacgg
      961 tttcatcgga aacctctccc gggcggaaac cggcgatctc gcagaatagt tcgatcctct
     1021 tctgacccgc ctctgcgata ctggaaacag cagtgattat gtcttcattt tcgtaaagga
     1081 gagtcgatac accgatattc aagacttgta atcccccatc cctgtgtcta atctatgaac
     1141 actgtttcta tgtgaagaat gcaaaacctc tcagcccgag ttgagcatca caggaaaacc
     1201 cgtctttcac gtcgggcaca gcaggcaacg gcatagctcc gtgaatgtca cagggcgcca
     1261 caaaatgcta actccctatt tcgcaatgtt cctgtgcttt tcgacagttc gatttcaatc
     1321 cagtaccgcc gccacagcct atcacatctt tgactccgtt caatctaaat cgcgacatgc
     1381 tgcggtcgaa ggccccatac tgtcactcaa gtatgaccat ggcaagcacg actcgcaatc
     1441 ggcgcgatag agcatatacc cgaagcctct cgataactgc agacaaaaac tgtagattgg
     1501 aataaatcaa tggaactttc ttcttgactc aagctgtctc aaaacatata tttttagata
     1561 ccacatcact tgaaccacat gatatctagc gctttttctc tcaatgctca atgccaacag
     1621 gggggggaca taaccttgac ctgttcagct attacctcaa taatggcatc tcttctctct
     1681 tcggtttcta tgatgtctac aacctacagg tctctgtggc agctaccctc ctttaacaga
     1741 agtatagcga tccttgcctt gggagccttc gccgtggcat gcgacgcggt agtccgcacg
     1801 tttttttcga catcgtccat agacagaagg ccttgcttgg ctacagccat agtcccgtta
     1861 ttctcgtcct tctctcctcc agatgccaaa ggtccccagc caagacatta tgccctttcg
     1921 cggacccgaa ctggatcgag tgaaggcaac gcatgcgagc caacaggccg accctggttg
     1981 ccgaagctct ttttattcac catttccctc acgtcattaa tgcgcgaact attagataga
     2041 tctctccgag tcgtgaagca acttcttcca tggatagcga ggtccggtac aggagagcaa
     2101 ttgcacgtca agcggggcgc gtcacagcgc gacgatatcg gatgcattgc caatatagcg
     2161 ctggattacg gcatcgactt ggcgttcgac tggtcatctc ctctcatcct cactccgttt
     2221 gtggaatgga ccgagccatc gatagacaac gccttaagct ccgtcatgcg cctgttaacc
     2281 ctttctgaag ttgcaagaga tgaggcaatc agctgtaagt tagtggagat acaaagatct
     2341 cttcatgcag ctaaatcttc gttgagatta cgcgaccgcc ttagtccgca ggaaaaaggc
     2401 ctgtttgacc aacgactgcg tgaacgagta agtagactca atgtgatgct gagggggtag
     2461 tcggttatga cgaaccgaaa agacttgctt gatatgccag tgatgaccat agatgagggg
     2521 gcagaggcag ggcgcataac cgggctggtg atcgatccca tcagtgtgca cgctgttgcg
     2581 ctcatcgtct ctaccaacga gcgtttcaac gccccgaaag cagttccgtt tccattggta
     2641 caaggtctag gtgaagacgc agtgatgatc gagaacaaat cggcaatggc cgagttctcc
     2701 tcgctgccgg aactgactac cattggaaaa acagattcgg aattgtgtgg actcacagta
     2761 atagcccgca ccgggaagat aataggatat gttgaggact ttgccgtgga tgatgagaca
     2821 ggcagaatcg aatcattctt ggtgcnnnnn nnnnnaaaaa acgtcatcac cataggctcg
     2881 aatgccctta ttgtgatagc tgacactgtc gacgctctag attcataaca cgcgcacagt
     2941 ccatcaggtc tcagacttgg aggtctgtga aatgcgcatg cccacgcacc ctgtcgctcg
     3001 caggctggcg atggcggcaa tcgctgttgt cagcatagtg gtgcaagacg caactgctgt
     3061 ggtacgactc cccatgacaa caaataagtc tccagagtgc atcaaccctt tggcccacac
     3121 atgccatgta ctgccttgct tggattcaaa ggcccggttc gagaattcag aaggcgcgag
     3181 atggcggccg gaaaagaagg ggctctcagg gatcgtagcc ggaagcggaa tgctatctgt
     3241 tagggatcgc acgtca
//
LOCUS       DAOSAI010000299         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_19007_length_3241_cov_3.058067, whole genome shotgun sequence.
ACCESSION   DAOSAI010000299 DAOSAI010000000
VERSION     DAOSAI010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19007_length_3241_cov_3.058067"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3120)
                     /locus_tag="PLM83_09190"
     CDS             complement(<1..3120)
                     /locus_tag="PLM83_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing
                     serine/threonine-protein kinase"
                     /protein_id="HOK71612.1"
                     /translation="MGDTRKCRGCGTQLTSNASSDLCPKCALEAKSHKQRGERTEYDR
                     NLLFGVLAVQLRRVTPAQLIEVAAAWASEPSLDLATRLVNTGLISEKDRDLILGFIDQ
                     AVQAHGGDTKATLSAFGGEEQIHQSFGGSIERTESGGIRSVTPTVEVLEEIDPDAVPG
                     VYEPPGRYTMLREHAQGGMGRILLVHDKHLSRDIALKELLPAPVPSTSEGQPSPVRLS
                     VPWIARFLQEARIAGQLEHPAIVPVYELGHRRDGTLYYTMKLVRGKTLAQAIKEASSL
                     RERLDLLPHFVDLCQAIAYAHSRGVIHRDIKPGNVMVGEFGETVVIDWGLAKARGRKD
                     IHAEEMAETIQVMRLGDEAAVTKTAYGQAIGTPLYMSPEQAQGDIDAVDERSDVYSLG
                     TVLYEILTGKPPFSGSSTREILSKVINDKPEPLLTAQPDTPPELAAICARAMARDPAR
                     RYPSAKDVAEEVQRFLSGALVQAYQYRFSEHLRRFVSRHKATLTTAAIALLALVGVGI
                     YSYTNIVRARGLERQLRLEAEVARTQAEQAREEAVKAREAAEALAYQSSVRLLHQYVN
                     SQNYGFADEFVWKLPEAYRNWEWGHCLGLLHQELFSVNGNFVEVNPQGSLILTYSQNH
                     HEVHELTSGELLWTEPRQDIAWARFTADEERILVAKKSDDHYELMSVRLPFNSVERVL
                     RYPPNSRGKAVMSPDRTRLAHIFDNDMATVKVFDVDSLQEIATFSDRKVPISFVKFSP
                     DGTKILTVSKDQNAILWDVNTGTRLWSVSTGGITAFSPNGEALLVTSPEGDLVLWNTT
                     TRERLWSASPFYQERVTTCVFSPDGFLLAVGCTGGSVKVLGAENGSEVKSFEGFPDAI
                     STLVFDMQSRRLFIGSRDGIAKIWDVKAGEELAYLSLQPREGRIVPNMIPQAAFTPDG
                     TRVVLGFVWSDQTTIWDVRGPFDRLSLSPTMAPQCPRGYQGFPFGSDGSRIALLSYDA
                     AAVWDSDLRQRVIIIWPFPWRAALSPDGNRLLITSGSSGIVWDIERQEHPRANMIEGL
                     SSRHDNATAF"
BASE COUNT          780 a          873 c          841 g          747 t
ORIGIN      
        1 gaatgccgtg gcattgtcat gcctagagga aagcccctcg atcatattcg ctctgggatg
       61 ctcttgccgc tcaatatccc aaacgatgcc agaggaaccg gacgttataa gaagccgatt
      121 cccatcggga cttaatgccg cgcgccatgg aaaaggccag ataatgatca cgcgttggcg
      181 caggtcagaa tcccaaactg ctgcggcgtc ataactcaga agagcaatac gactcccatc
      241 agagccaaac ggaaacccct gataaccgcg cggacattga ggagccattg tcggtgagag
      301 tgaaagtctg tcaaaaggcc cgcggacatc ccaaatggtc gtttggtcgc tccagacaaa
      361 gcccaaaacg acgcgtgttc catcaggcgt aaatgcagct tgcggtatca tgtttggaac
      421 aatcctacct tcacgtggtt gaagagagag ataggcaagt tcctccccgg ccttgacatc
      481 ccagatcttc gcgatgccat ctcttgaacc gatgaaaaga cgcctgcttt gcatgtcaaa
      541 cactagagta gatatcgcgt ctggaaatcc ctcgaaggac ttcacttccg aaccgttctc
      601 agccccaaga accttcacag aacctcctgt gcaacctacc gccaaaagaa aaccatcggg
      661 actgaacaca caagttgtga cgcgctcctg ataaaatggg gacgcactcc aaagccgttc
      721 tctcgtagtg gtattccata ggacgaggtc cccctctgga gaggttacaa gaagggcttc
      781 tccattagga ctgaacgcag ttatcccccc ggtcgagaca gaccatagcc gcgtgccagt
      841 gttgacatcc catagtatgg cattttgatc tttggagact gtaaggatct ttgtcccatc
      901 cggactgaat ttgacgaaag atattggcac cttacggtcc gaaaaagttg ctatttcttg
      961 taagctatcc acatcaaaca cttttaccgt ggccatatcg ttatcgaata tatgggcaag
     1021 cctggtgcgg tccggactca ttactgcctt cccccttgaa tttgggggat accgtagaac
     1081 tctctcaacg ctattaaaag gaagcctgac tgacatgagt tcgtaatggt catcagattt
     1141 ttttgctact agaatgcgtt cctcatcggc agtaaacctc gcccaagcta tgtcctgtct
     1201 gggttctgtc catagcaact cgccgctagt aagctcgtga acctcatgat gattctgcga
     1261 gtacgtgaga attagggaac cctgcggatt aacttccaca aagttaccat tgacagagaa
     1321 aagttcctgg tggagtagtc cgaggcagtg tccccattcc caattgcgat acgcttcagg
     1381 caatttccaa acgaattcgt cagcgaaacc atagttttga gagttcacat attggtgcaa
     1441 caaacgcaca ctcgactggt atgctaaagc ttcggcagcc tcgcgggctt tgacggcttc
     1501 ctcacgtgcc tgttcggctt gggttcgggc gacttctgcc tcaagccgta gctgtcgttc
     1561 gaggccgcgc gctcgcacaa tgttcgtata ggagtaaatg ccgacaccta cgagggcaag
     1621 aagggcgata gcagccgtgg tgagggtagc cttgtgacgt gagacgaagc ggcgtaaatg
     1681 ctcggaaaag cggtattgat aggcttggac gagggctccc gataaaaaac gctggacttc
     1741 ttccgccacg tccttcgcag aaggataacg tcgagcaggg tctcgcgcca tagcgcgtgc
     1801 gcagattgct gccagttcag ggggtgtatc gggttgtgca gtcaggagag gttcgggctt
     1861 gtcattgata accttggaaa gaatttcacg agtgctgctg ccactaaatg gtggcttgcc
     1921 cgtaagaatc tcgtaaagca ctgtgccgag cgagtacacg tccgaccgtt catctaccgc
     1981 atctatatca ccctgagcct gctccggcga catgtacagg ggagtcccaa tagcctgacc
     2041 ataggccgtt tttgtgacag cggcctcatc gcctagccgc atgacttgaa tggtctctgc
     2101 catctcctcc gcatggatgt ctttgcgccc ccgagccttg gctaagcccc agtcaataac
     2161 gaccgtttcg ccgaattcac ccaccatgac gttgccgggc ttgatatcac ggtggataac
     2221 gccgcgacta tgggcgtacg caattgcctg acacagatca acaaagtgcg gcaatagatc
     2281 aagccgctcg cgtaatgacg atgcttcttt aattgcttga gccaaggttt taccacgaac
     2341 gagtttcatt gtgtaataca aggtaccgtc cctgcgatgg ccgagctcat acacgggcac
     2401 gatggctgga tgctcaagct ggcctgcaat ccgtgcctcc tgcaaaaaac gagcgatcca
     2461 aggaacagac agtcgaacgg gactcggttg accctcggat gttgacggga cgggggcagg
     2521 caggagttct tttagagcaa tgtcccgact taaatgcttg tcatggacta agagaattcg
     2581 tcccatacca ccctgggcgt gctcccgcaa catcgtgtaa cgcccgggcg gttcgtagac
     2641 gccggggact gcatcggggt ctatttcttc gagcacctcg acggtaggtg taacggaacg
     2701 gattccgccg gattccgtgc gctcgataga gccgccgaaa gattgatgga tttgttcctc
     2761 gccgccgaac gctgacaggg tcgctttcgt atcgccccca tgggcttgaa ccgcctgatc
     2821 gatgaatcca agaattaagt cgcggtcttt ctcagagatg agccccgtgt tgaccagccg
     2881 tgtcgcgaga tccagcgaag gttcggacgc ccaagcagca gcaacttcta tcagttgagc
     2941 gggtgtcacc cgcctgagtt ggacagccag tacgccaaaa aggagattcc tatcgtattc
     3001 ggtacgctct cctcgttgct tatggctttt ggcctccaac gcgcacttag gacacaaatc
     3061 tgaggatgcg ttggaggtca gttgcgtccc acatccgcgg cactttcgtg tatcgcccat
     3121 cgctccacct gcccagatcc actcaacacg aaagagaaaa ctgtgtgaag tcttcaatat
     3181 accgaaagag ggaggggaat tccagattgt gtataagttc agtacctttg gcactttttg
     3241 t
//
LOCUS       DAOSAI010000300         3219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_19201_length_3219_cov_3.549621, whole genome shotgun sequence.
ACCESSION   DAOSAI010000300 DAOSAI010000000
VERSION     DAOSAI010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19201_length_3219_cov_3.549621"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1663
                     /locus_tag="PLM83_09195"
     CDS             143..1663
                     /locus_tag="PLM83_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOK71613.1"
                     /translation="MDKEKYLTGADPLDIPLEQFDAPLFNKLLWYVKEDPVRFHMPGH
                     KGVGYPPEVLEAFSENMLSMDVTETRDLDNLHLPTGCIKQAQDLASEVFGADRTFFLT
                     NGTTAGIHAMLMTSCKEGQKVIIPRDAHLAVVGGVILAGLAPVFVYPDIDPEWGISMG
                     VRPEAYEQALTENPDAKACLITRPNYYGIAGDLAPLAEICHQRDIPLLVDEAHGPHIR
                     FHEDLPAPALEYGADMVVQSTHKTLGSFTGSSMLHIKGPRIHPDRVQRMLAILQSTSP
                     SYLLMASIDIAATIMRLRGPQLIDRALKLSDKARRRLDGILGIRVLWRPGMDTLRLTV
                     SMIQLGLAGYDLKNRLMDECNVRIELADLENIVAFITYADSEENVDRLVDALLQIADE
                     ARSEMTGKLGVRTSGNQAEFTRHLFAPAERACLPRQATLAPHELVEVKDAVGRVCAEV
                     VAPYPPGIPLLYPGEIIDGLHLDIIEEALSLGAVFRGLSFGPDGQLIVTVVKDGKR"
     gene            1663..2310
                     /gene="tmk"
                     /locus_tag="PLM83_09200"
     CDS             1663..2310
                     /gene="tmk"
                     /locus_tag="PLM83_09200"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOK71614.1"
                     /translation="MTQRGVFITLEGPDGSGKSTQAGLLAERLESEGYPVTRTFEPGG
                     TSIGKRLREIILDNKSTGMCGVAEFLLYAADRAQDVHEVIEPALEAGRIVVAERFVDS
                     SIAYQGYGLGMDIDEVKQVNRIATRGLVPDLTLLLDIDPAIGVRRATDCHEADRIEQR
                     ELDFHVSVREAYLMMAAAEPERFMIIETGGLCVLEVHEEIYTAVTKLLETRAWED"
     gene            2314..>3219
                     /locus_tag="PLM83_09205"
     CDS             2314..>3219
                     /locus_tag="PLM83_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOK71615.1"
                     /translation="MHIPEIVGQDEALRLLDREMASGRMSHAYLLWGPRGSGKMALAK
                     RVALAANCMNCGAGNAAGDRSERGPDSPLWYCGNCSNCEKILRGVHPDVCVVKPEGGA
                     IKIDQIREMKWRISLKPNEADLKTWIIDEADRMTEEAQNCLLKVLEEPPGDSLIILAA
                     EDPGMMLPTISSRCHEARLKAVPVEELARWGQSELGLDPERARLLATLSEGLPGRLVC
                     LAQDDDYFKVRDLIISTVRAVALCGNGAEALAASEEFLDKLKKASAQAVDGGGSVEVQ
                     ELASPMGACDLAAGWFRDLFILSITG"
BASE COUNT          741 a          786 c          974 g          718 t
ORIGIN      
        1 ccccctaact cttgggcaat aaagagaaca catgttctac gactagatat taacatagaa
       61 catatgttcg tgtcaagctt accctaaagt cgtgccaata atccccgggc accggggcat
      121 aaaggagaag ataagatcag aagtggacaa agaaaaatat ctaacaggcg cagatccact
      181 cgatatccct ctagagcaat tcgacgcccc gcttttcaac aagttgcttt ggtacgtcaa
      241 agaggatccc gttcgttttc acatgcccgg gcacaaaggt gtgggctatc ctccggaggt
      301 tctggaggcc ttcagcgaga acatgctcag catggatgtg accgagacgc gggacctgga
      361 taatctccat cttcctaccg gatgtataaa gcaggcgcag gatctggcat cggaggtatt
      421 cggagctgac cggacgtttt ttttgactaa tggaaccacc gcaggaatcc atgctatgtt
      481 gatgacaagc tgcaaggaag gacagaaagt gatcatcccg cgtgatgccc acttagctgt
      541 ggtaggtggt gtgatcctgg ctgggctggc gcctgtcttc gtgtatcccg acatagatcc
      601 cgagtgggga atcagcatgg gggtgaggcc tgaagcgtac gagcaggctc tcactgagaa
      661 tcccgatgcc aaggcttgcc tgatcacgcg ccccaattac tatggcatag caggagacct
      721 tgcgccgttg gcagaaatct gtcatcaacg cgatattcca ttgcttgttg atgaggctca
      781 tggaccgcac atacgctttc acgaggatct tccggctccc gcgttggagt atggcgcaga
      841 catggtggtc caaagcaccc ataaaacgct cggttcgttt accggaagct ccatgctcca
      901 catcaagggg ccccggatcc atcccgatcg cgttcagcgt atgttagcga tactccagtc
      961 cactagtccg tcatacctcc tcatggcctc gattgacatc gctgctacaa tcatgaggtt
     1021 gcgcgggccc caacttatcg atagagctct caagctttct gataaggctc ggcggcgtct
     1081 ggatgggatt ttgggcataa gggtgttgtg gagaccgggc atggatacgc ttagactgac
     1141 agtctcaatg atccagttgg gattggctgg atatgatcta aagaaccggt tgatggatga
     1201 gtgcaatgtc aggatcgaac ttgcggacct cgagaatata gtcgctttta taacctatgc
     1261 cgactctgag gaaaatgtgg atcgccttgt agatgcgctt ttgcagattg ccgacgaggc
     1321 gcgcagcgag atgacaggaa agctcggcgt aagaacctcg ggaaaccagg cagagttcac
     1381 ccggcatctg tttgcgcctg cggagagagc ttgtcttcct aggcaggcaa ctcttgctcc
     1441 gcacgaactg gtcgaagtta aggacgccgt cggcagggta tgcgccgagg tggttgcccc
     1501 atatccgccg gggataccgc ttctttatcc aggagagatc atcgacggct tgcatctcga
     1561 tatcatagag gaagcgttgt cccttggggc ggtcttccga ggcctttcgt tcggcccaga
     1621 tgggcagctc atcgtgaccg tggtgaaaga tgggaagcga tagtgacaca acgtggagtt
     1681 tttataacct tggaagggcc tgatggcagc ggaaagagta cgcaggccgg tctattggca
     1741 gaacgccttg aatcagaagg gtatccggta acgcgcactt tcgagccggg cggcacgtct
     1801 atagggaaga gattgcggga gatcatcctg gacaataagt ccacaggaat gtgcggggtt
     1861 gcagagttcc tgctttatgc tgcagatcgt gcacaggatg tgcatgaagt aatcgagccg
     1921 gctctagagg cgggcaggat agttgtggcg gagcgttttg tggattccag catcgcctac
     1981 caaggatacg gtcttggcat ggatatcgat gaagtgaagc aggtgaaccg gatagccact
     2041 cgcggcctgg ttcccgatct tacgttgctg ctcgacatcg atcctgccat cggtgtcaga
     2101 cgtgcgaccg attgccacga agccgacagg atcgagcagc gcgagctcga ttttcatgtg
     2161 tcagtccgcg aggcttacct aatgatggcc gctgcggagc cggagcgttt catgattatc
     2221 gagactggcg gactatgtgt tttggaagtg cacgaggaga tatacactgc tgtgacgaag
     2281 ttactcgaaa cgcgtgcgtg ggaggactga gggatgcaca ttcctgaaat cgtcggtcag
     2341 gatgaggccc tccgattgct cgatagggag atggcttcgg ggcgcatgtc ccacgcttat
     2401 cttctgtggg ggccacgggg aagcggcaag atggcgctgg caaaacgggt tgctctcgct
     2461 gcaaactgca tgaactgtgg ggcggggaat gcagccggag accgatctga gcgcgggccg
     2521 gatagcccac tgtggtattg cggcaattgt tcgaattgtg agaagatact gagaggcgtt
     2581 catccggatg tttgtgtggt gaaaccggag ggaggcgcca taaagataga ccagatcagg
     2641 gagatgaagt ggcgcatatc ccttaagcct aatgaagccg acctcaagac atggataata
     2701 gatgaagcgg atcgcatgac tgaagaggcc cagaactgcc tcctcaaagt gttggaggaa
     2761 ccccctggtg actcgttgat catccttgcg gcagaggatc cgggcatgat gttgccgaca
     2821 atctcatcgc gttgccatga ggcacgcctc aaagctgttc ccgttgaaga gcttgccaga
     2881 tggggccaga gcgagctcgg tctcgatcct gaacgggcca ggcttctggc tacgctttcg
     2941 gaagggcttc ccggccgcct ggtatgcctg gctcaagacg acgactattt caaggtaaga
     3001 gatctcataa tatccacagt gcgcgcagtg gccttgtgtg ggaatggtgc cgaggcgctt
     3061 gctgcttcgg aggaattcct cgacaagctg aagaaggcta gtgcgcaagc tgtagatgga
     3121 ggagggtccg tggaggttca ggagctcgct tctccaatgg gggcatgcga ccttgcggcc
     3181 ggatggttca gagacctctt catcctgtcc ataacaggt
//
LOCUS       DAOSAI010000301         3214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_19244_length_3214_cov_3.933840, whole genome shotgun sequence.
ACCESSION   DAOSAI010000301 DAOSAI010000000
VERSION     DAOSAI010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19244_length_3214_cov_3.933840"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLM83_09210"
     CDS             complement(<1..262)
                     /locus_tag="PLM83_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HOK71616.1"
                     /translation="MVERKLFKGNEAVAEAAVRAGCRYFFGYPITPQNEIPEYMARRL
                     PEVGGLFLQAESEVAAINMVYGAAGTGARVMTSSSSPGISLKQ"
     gene            complement(277..483)
                     /locus_tag="PLM83_09215"
     CDS             complement(277..483)
                     /locus_tag="PLM83_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK71617.1"
                     /translation="MTKVVFDEEKCKGCGLCVQFCPKKAIIMAERINTKGYHPAVLAD
                     EENCISCGICARMCPDAVIEVFKP"
     gene            complement(480..1568)
                     /gene="buk"
                     /locus_tag="PLM83_09220"
     CDS             complement(480..1568)
                     /gene="buk"
                     /locus_tag="PLM83_09220"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOK71618.1"
                     /translation="MSDFRVLVINPGSTSTKVAVFEGDRSAASESLRHDADMIASYPR
                     IFDQFDMRKEAVFDFMERLPFPADTLSAVVGRGGVLPPMEGGVYIVDDVLVDALYEWG
                     WNHASSLGGIIAKEIADSLGIPSYIADPTTTDELDNIARMTGIPGIERRSVFHALNQK
                     AMARKAARDLGRKYEECNFIVVHLGGGISIGGHKRGRVVEVSNALDGEGPYSSERSGS
                     LPNTSLVRLCFSGRYTEAEMRRKITGQGGLVAYLGTNSLLDVEKKIEEGDDYARICYE
                     GMAYQVAKEIGRVACALSGDVDAIILTGGAANSKMLVSWIEERVKFIASVMVYPGEAE
                     MEALADAGLRVLRGLEEPKRMMLGGGGR"
     gene            complement(1586..2482)
                     /gene="ptb"
                     /locus_tag="PLM83_09225"
     CDS             complement(1586..2482)
                     /gene="ptb"
                     /locus_tag="PLM83_09225"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050182 - phosphate butyryltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HOK71619.1"
                     /translation="MLAAFDEVVARAKKRPSAKVAVAVAQDREVLEAIQMAQGIGMGA
                     FVLVGDEKLIRPLASAVGLDLSELEIVHEPDARTAALHAVELVSSGQAGILMKGLIPT
                     ADFLRAVLDKEVGLRTGKLLSHVAVFKSPRYDRLIYMTDGAMVVAPTLQDKVQIIQNA
                     VGVAHKLDNEVPKVACIAAVEVVNPDMPETVEAAALAKMCDRNQIRGCIVDGPLGLDN
                     AVSEESARHKGVDGPVAGRADILLVPNIVAGNVIYKTLVYFGQVETAAILTGARAPIV
                     LTSRSDSPEARLNSLALAIATS"
     gene            complement(2530..>3214)
                     /locus_tag="PLM83_09230"
     CDS             complement(2530..>3214)
                     /locus_tag="PLM83_09230"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOK71620.1"
                     /translation="ISVDEYEPLARLSGLPEISRVSKLHALSIRSTAAKAAASLGKAL
                     EEANLVVAHLGSGISISPVKAGKMVDANGADDEGPFSPERSGSVPMSDLVTLCYSGQY
                     DEGELIHLLTHGSGLFAHLGTTDAREVEKMILAGNTRASDVEQAMAYQISKEIGAMAT
                     VLCGRVDAIVLTGGLAAWDRLVGHITERCCFIAPVITYPGENEMEALASSVMRVLAGE
                     EKARVYASV"
BASE COUNT          739 a          991 c          723 g          761 t
ORIGIN      
        1 cctgcttgag actaatgcca gggctcgatg atgaagtcat tacgcgcgct ccggttccag
       61 cggccccata aaccatgttt atggccgcta cttcactctc agcctgcaga aacaggccgc
      121 cgacttcagg caaccttcgg gccatgtact cagggatttc gttctgggga gttatcggat
      181 acccgaagaa gtacctgcac ccagccctga cggcagcctc agcaacggct tcatttcctt
      241 taaacaattt gcgttctacc atgcatactg ccctcctcag ggtttgaata cttcgataac
      301 agcgtcagga cacattcgtg cgcaaatccc gcaagaaata cagttctcct catctgcgag
      361 gacagcgggg tgatagcctt tggtgtttat cctctcggcc ataattatcg ctttcttggg
      421 gcaaaactgc acacacaacc cacaaccctt gcacttttcc tcgtcaaata caactttcgt
      481 catcggccgc ctcctcccaa catcattcgt ttgggttcct caagaccccg gaggaccctg
      541 aggcctgcat cggccagcgc ctccatctct gcttcaccag gatacaccat cacgctggca
      601 atgaatttga ctcgctcctc aatccaggac acaagcattt tcgaattcgc agcgcctcct
      661 gtaaggatga tcgcatccac atctcctgaa agggcacaag ccacacggcc tatctccttt
      721 gctacttgat aggccatacc ttcatagcaa atcctggcat aatcatctcc ttcctcaatc
      781 ttcttctcaa catccagcaa cgaatttgtt ccaagatatg ccacaaggcc gccttgtccg
      841 gttatcttcc ttctcatttc cgcctctgtg tatcttcctg agaagcagag cctcaccaaa
      901 gacgtattgg gaaggctacc ggaccgctca gatgaatatg gaccttctcc atccaacgcg
      961 ttgctgacct cgacaacgcg cccgcgcttg tgtccgccta tgctgatgcc cccaccgagg
     1021 tgaaccacta tgaagttaca ctcctcatat ttccgcccca aatcgcgcgc ggctttgcgc
     1081 gccatcgcct tttgattgag tgcatggaag acactccggc gttcaatccc gggaatgcca
     1141 gtcatgcggg caatgttgtc aagctcgtca gttgttgttg ggtcagcaat ataagaggga
     1201 atccccaaag aatcggctat ttccttggct atgattccac caaggcttga agcgtgattc
     1261 catccccatt catacagggc atccaccagc acatcatcta cgatgtagac tccgccctcc
     1321 atgggaggca ataccccacc acgtccaacc acggctgaca gagtatctgc aggaaacggt
     1381 aacctttcca tgaagtcgaa caccgcttcc tttcgcatat caaactgatc gaagatgcgg
     1441 gggtaggaag ctatcatatc ggcatcatgt ctgagacttt cggacgccgc tgaacgatct
     1501 ccttcaaaga cggctacctt cgttgaagtg gacccggggt taatgacaag aaccctgaaa
     1561 tcgctcactc caaccacctc cgttactatg acgttgcaat agccagcgcg agcgagttca
     1621 gcctggcttc aggagagtct gagcgcgacg tgagcacgat tggagcacga gctccggtca
     1681 gtatagccgc ggtctcaact tggccgaagt aaaccagggt tttgtatatg acgtttcctg
     1741 caacaatgtt tggaacaagc aaaatgtctg ctctgccggc cacagggcca tccactccct
     1801 tgtggcgtgc agactcctca gagactgcat tgtcgaggcc aagagggcca tctacaatgc
     1861 atccgcgtat ctggttccta tcgcacatct tggccagcgc tgccgcttca acagtctcag
     1921 gcatgtctgg attgaccacc tcgacagcgg ctatgcacgc aaccttgggc acttcgttgt
     1981 cgagcttgtg tgccaccccc actgcgttct gaattatctg tactttgtct tgaagcgtgg
     2041 gcgccactac catagcacca tcggtcatgt aaatcagcct atcgtacctc ggagatttga
     2101 agaccgccac atggcttaat agcttcccgg tccggagacc aacctcctta tctaaaacag
     2161 ccctaaggaa atcggcagtt gggatcaggc ctttcatcaa tatgcccgcc tgcccagagc
     2221 tgactagttc aaccgcgtgc agggcagcag tcctggcatc aggttcatgg acaatttcca
     2281 attcagaaag gtcaaggccc actgcggacg ccagcggtcg tatcaacttc tcatccccta
     2341 cgaggacgaa cgcgcccatc ccaatcccct gagccatttg aatggcttcg agcacttcac
     2401 gatcttgtgc aacagcaact gccaccttgg ctgaagggcg cttcttcgcg cgcgctacaa
     2461 cctcatcgaa agctgcaagc atcgacatct cctccctcag gtatgtcttc gcatcaaatc
     2521 ccaatctgat cagactgatg catatactct tgctttttcc tccccggcaa gcactctcat
     2581 gacgctgctc gctaaagctt ccatctcgtt ctcgccagga taggtgatta cgggcgctat
     2641 gaagcagcat ctctctgtga tgtgtccaac cagacgatcc caagctgcaa gccctccggt
     2701 gagaacaatc gcgtcaacac gcccacaaag aactgttgcc atggctccta tttccttgga
     2761 tatctggtag gccatggcct gctctacatc tgacgcacgc gtatttcccg caaggatcat
     2821 cttctcgacc tcgcgagcat ctgtagtccc tagatgtgca aaaagcccgc ttccatgggt
     2881 gagtagatga atcaattcgc cctcatcata ctgccccgaa taacacaaag tcaccagatc
     2941 gctcatgggc acagagcctg agcgctccgg cgagaacggg ccttcatcat ccgccccatt
     3001 ggcgtccacc attttaccag ccttgaccgg ggatatcgat atcccgctgc caagatgggc
     3061 gaccacaagg tttgcctctt ccagcgcctt gccgagactc gcagcggctt ttgctgcagt
     3121 gcttcgtatg gaaagtgcat gcagtttgga tacccgcgag atctccggca atccggatag
     3181 tctcgcaaga ggctcatact catctactga tatt
//
LOCUS       DAOSAI010000302         3194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_19398_length_3194_cov_2.955718, whole genome shotgun sequence.
ACCESSION   DAOSAI010000302 DAOSAI010000000
VERSION     DAOSAI010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19398_length_3194_cov_2.955718"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..>439)
                     /locus_tag="PLM83_09235"
     CDS             complement(81..>439)
                     /locus_tag="PLM83_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71621.1"
                     /translation="LDARKIRGLMDQAEFMIAHNASFDRPFVCRIFPEADAKPWVCSC
                     KEISWRRYGFGSARLQELTRACGIRAQQTHRALDDVSDAIELLRQTNSFTGATFFSEL
                     LRAGQADGRISRGRGI"
     assembly_gap    440..449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<450..694)
                     /locus_tag="PLM83_09240"
     CDS             complement(<450..694)
                     /locus_tag="PLM83_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOK71622.1"
                     /translation="MNKRTSASQAMEFSILGSACFLDVETTGFSPRTDEIVELAIVLF
                     EFDYADGAIVKIVDTYSGLREPSVPIHPGASRVHGLT"
     gene            complement(1111..1557)
                     /locus_tag="PLM83_09245"
     CDS             complement(1111..1557)
                     /locus_tag="PLM83_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HOK71623.1"
                     /translation="MKQAGARVWGPSPVGTLDYITWDDVVLDTDYNVNVYWAATQRRL
                     VPFLSDEYVSFTVLTLCRILATLKRGEIVSKRAAGEHAMETLPPTWKPLICEAIDLHS
                     ASCSAAVVSDKWLGNLFRRIRRARGTQRFLAYMLRVLRDELNQRNG"
     gene            complement(1718..1918)
                     /locus_tag="PLM83_09250"
     CDS             complement(1718..1918)
                     /locus_tag="PLM83_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71624.1"
                     /translation="MHEKNPATAALAEYAIMVNARLPGLIHGVYANDSALVGDFDLAK
                     LRQRDPREGERWRTQGPNGNQQ"
     gene            2477..3016
                     /locus_tag="PLM83_09255"
     CDS             2477..3016
                     /locus_tag="PLM83_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71625.1"
                     /translation="MDSWYVMLFPNGVPESFGATVSLLQQYVGGSLVARPHVTIAYLE
                     GNERPEILVDRLKGLSSPTVPIHGRGLFSFWPDSQHELFGYTLFMHAEKTAALERLHA
                     MAIRAIEGTGIPPARSWEDTNLHVRLLDHMPMSPSKALPRLPQDDWAISFPATRLILS
                     QSAEDGSYTEWLDLPLRSD"
BASE COUNT          723 a          944 c          807 g          710 t
ORIGIN      
        1 catgtcagct aaccttgccc gagccacaca accaaccacg taaccccaca aaacctcgca
       61 tccacgacct actcgtaacc ctatataccc cgcccccgtg aaatcctccc atcggcctgc
      121 cccgcccgga ggagttcgct gaagaatgtg gcgccggtga aggagttggt ctggcgcaac
      181 agctcgatag cgtctgagac atcgtcgagg gcccggtgtg tctgctgggc gcgaattccg
      241 cacgccctcg taagctcttg cagtctggct gaaccgaaac catatctgcg ccaagaaatc
      301 tccttgcacg agcagaccca tggctttgcg tctgcctccg ggaatattct gcagacgaag
      361 gggcggtcaa aggaggcatt gtgcgcaatc atgaactccg cctgatccat caggcctctg
      421 atcttcctcg catcgaggcn nnnnnnnnna ttgtcaggcc gtggactctg gacgctccgg
      481 ggtgtatggg gactgatggt tccctcaggc ctgagtatgt atcgacgatc ttgacaatag
      541 ccccgtcggc atagtcgaac tcaaacaaga ctatcgccaa ttccacaatc tcgtctgttc
      601 ttggagaaaa gcctgtggtt tcaacgtcta gaaaacatgc agagccaagt atggagaact
      661 ccatggcttg actagcagac gtgcgcttgt tcaatatcaa cactcccaat ccctatctgt
      721 catctgtttc acaatggcaa agctctgcga acacgcgggt gaaattgcta ttccaatact
      781 accttcagtc agccccgata aggaggctat cagctggcct ctccaagctc ccggcgagcc
      841 ctctcgagca tctcctggat cacgaaacac gtcgccgccg catatgccgg ctggtcagtt
      901 ctcacgcttg caagttttcg ccatctcgcc gttgcgctcc ttcaccattg cttcacccac
      961 acattcgcct ccaccttcga ctgcagacca cagtcgctgg ccagaaagca ggcgccgcat
     1021 caattctgaa gatgcaccac attgggcaga gagtatgggc aacaacactc taggaccatg
     1081 atgcggatag atctcgctgc tcgctatctg tcagccattt ctctggttca gttcatccct
     1141 gagcactctc aacatatacg cgagaaacct ttgtgtccct cgagctcgcc tgatccgtcg
     1201 aaacagattc ccaagccact tatcactcac gacagctgct gagcaagacg ctgaatgcag
     1261 gtcaatggct tcgcatatca acggtttcca cgtgggcggc aacgtctcca tagcatgctc
     1321 tccggcggcc ctctttgaga ctatctcgcc gcgtttcagg gtcgcgagga tgcgacacag
     1381 cgtcagaaca gtgaatgata catactcgtc tgaaaggaac gggaccagtc gtcgctgtgt
     1441 agcagcccaa tacacgttga cattgtagtc cgtgtcgaga actacgtcat cccaggttat
     1501 gtagtcgagg gtccctacag gcgatggacc ccatacacga gctcctgcct gcttcatctg
     1561 gtacaacgcc agacagggca cacgctcgcg cccgcaaaac gctgcgtcgc gatagcaaag
     1621 atgagggtcc tcaccgccag caatgcctgc ctgaagaaca ccgagtggaa tgtagtatcc
     1681 gtccattcta gccagaaggg aatgggtatc tgctagatca ttgttgattg ccgttagggc
     1741 cttgagttct ccatcgctca ccttcccttg ggtcacgctg acgaagtttg gccaaatcga
     1801 aatcaccaac taaggcggag tcgtttgcgt agactccgtg gataaggcct gggagtcggg
     1861 cattgaccat tatggcatat tctgccaaag ccgccgttgc agggttcttc tcatgcactg
     1921 caatcacccc caaaagcaag agggacaatc agaggtgcaa gacatgttca gagctccaaa
     1981 cttcttttcg agattcatct gccatcccct gccatagcag atggacgtca cggggataca
     2041 tggcttactt gctgctgagt gatgcttcct ggcgctggcg ttggcttcag ccatgtggct
     2101 gtgggaaggt gtgttgacgg gtaatccaat ggggccaatg tttgggagcc agatgacggg
     2161 cttgatcccg agataccaca tgaatggcat caccaacacc tcgctggcac gccgactccg
     2221 ttcaacgcag acgggaaatg tcagagtctt gttcacaggg gttgcccgct gcactgtgac
     2281 agcaagaagt gatattcccc cctaagcttc gcgtccccag gcacctacgc caacgccgtc
     2341 gtcagccgct acacgtcaaa tgtcattctg aggtcatgta gaatgatagg aattacgaca
     2401 tgattagaat ggctgtcgct tcgggacaag ctacttcaag caggggccaa aaagtgtaga
     2461 ccgtggagtg aataggatgg atagttggta tgtaatgctc tttccaaacg gggttcctga
     2521 gtcgtttggg gccactgtat ccctgctcca acagtatgtc ggcgggagtc tggtggctcg
     2581 gcctcatgtc accatcgcct atcttgaggg aaacgaacgc ccagaaatcc ttgtcgatag
     2641 actgaagggc ctgtcgagcc ctacggtgcc gattcatggc agagggctat tcagcttctg
     2701 gcctgatagc cagcatgagc tgttcgggta caccttgttc atgcatgcgg aaaaaaccgc
     2761 agcactagag cgcctgcacg ccatggcaat acgcgccatt gagggaaccg gcatccctcc
     2821 ggcacgctca tgggaggata ccaaccttca cgtgaggcta ctggaccaca tgccgatgag
     2881 cccttccaaa gccctgccca ggctgcccca agatgactgg gcaatctcat tccccgccac
     2941 acgccttatc ctttcccaat ccgcggaaga cggaagttac accgaatggc ttgacctgcc
     3001 gttgaggtct gactgaggtt cagcaatgcc cagttcccgc ccgggctttc agttgcgcca
     3061 cgtccaacca cgcagcgagg atttcctctt tcctcttagt cgtcctcatc ctcacaacgc
     3121 tttggcgcag ggggtatgta tccgtcttca tcagccgctg ctagccaaca ctaaatggct
     3181 tcgctcacac ttat
//
LOCUS       DAOSAI010000303         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_19534_length_3179_cov_3.814661, whole genome shotgun sequence.
ACCESSION   DAOSAI010000303 DAOSAI010000000
VERSION     DAOSAI010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19534_length_3179_cov_3.814661"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            810..2582
                     /locus_tag="PLM83_09260"
     CDS             810..2582
                     /locus_tag="PLM83_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71626.1"
                     /translation="MMTWGDYILRHITRLVVISRDYWRWMVIALVAMIGVTAANLAGP
                     WLIRSLVGTIEENFGHGTAAAGQVVNISIMLLLVYAVRPALRALQVWATHVAGWGSVA
                     AARKEIYEHLQRLSPKYYSSTQTGQIMSRAINDTAYFESLIAHVIPETIVSLLTVAGV
                     FVVLFSINPTLALYTLAPIPLIILGFVAYNRYVRPLFRHAQAKLGDLNAVLQDNLSGM
                     REIQAFTQEEREMESVGKRIMVHSSAITKAVSTSACFHGGIDFFAGLGTVSVVLFGGL
                     MAVQGQVSIADITGFLLYVGLFYEPIMGLNRLNESLQQALAASDRYFEVLDTQPDIDD
                     APDAVEFGRVEGCITYENVSFNYDETPVLKNINLTIKPGEMIALVGPTGVGKTTMANL
                     IPRFYDPTRGRVLIDGVDIRTATISSLRRNVGMVLQDVFLFNGTVAENIAYGSPKATL
                     EEIIEASIVAGADEFIREMPNGYDTHIGERGVRLSGGQKQRLAIARAILYDAPILILD
                     EATSSVDTETEAKIAAALDRLMAGRTTIVIAHRLSTIRHADRIIVLDEGEIVEEGTHE
                     ELMELGGLYATLVMADASTAGLAS"
     gene            complement(2853..>3179)
                     /locus_tag="PLM83_09265"
     CDS             complement(2853..>3179)
                     /locus_tag="PLM83_09265"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOK71627.1"
                     /translation="GQMAAGAAAVIGHILPVWFGFAGGKAIATFFGSCVLTAPVVVLL
                     AGVLWLVLYLVLRRVAQASAIASACLPLIGWVCRLPWQHILYLGVMGAAICLRHIPDA
                     CAPGEG"
BASE COUNT          792 a          910 c          773 g          704 t
ORIGIN      
        1 tctactagag aaccagtggc ctttccatac ccatccccac ccaccacaac agggcgaagg
       61 cctttttaca gcattatata ggacgtgact ttgtggcgtg gtggttatgc cttcttattc
      121 ttcagtttcc cctctaacat tttggctgcc gttctctgta tttctattat gccgaaaaca
      181 gatgcaatca ctcacaatct ccagaaacta cgcccagaag accgcgacgt aattctctat
      241 ttgatcaaga gattggccca gagttagttg tcaattacgc acctctgtcc acgattcaaa
      301 ataccctttg ccaagcttga gcctgagtct ttatccattc caacaactcc tttgtcttgt
      361 ccgtcaataa cccgcactcg ctgttttatc tcttcatgcc acatcagctt tgggcgcttg
      421 gacctggcga tattcctcat cctgctgaaa aaagcacaac catgttggca atatcgtcaa
      481 catcgaactt gcagcgatcc aaaacacgtc atccaatctg ggtcttcgca gcctgctgat
      541 gcgatctctc ttggcctcga gctcctcttt ggcatagccg ccgtcgttcc aaaacctacg
      601 actttgttca tcatgaggga ctgacggcaa catccccctt tcgcagcaaa taaggaaaat
      661 gcagatgaag agaaacgccg ttccgaagga actcttccag agaaggagaa cactaatgtg
      721 aatgccacaa gctctgagaa tccgctgggt tggagaagga tttgaacaac ttacacgctg
      781 cgtgattatg atatctgggc acacaaagaa tgatgacatg gggtgactac atattgcggc
      841 acataacccg tctcgtagtg atatcacgtg actactggcg ctggatggtg atagcgctcg
      901 ttgcaatgat aggcgtaacc gccgctaatc tagcaggccc ctggctgata aggagccttg
      961 ttggaacgat tgaagagaac ttcggccatg ggacggcagc tgctggccaa gtagtgaaca
     1021 tatccattat gctactcctc gtgtatgccg tgaggcccgc acttcgcgcc cttcaagtct
     1081 gggctactca cgttgctggg tggggcagcg tggctgcagc gcgaaaagag atctatgagc
     1141 atctgcaaag actgtccccg aagtactact cgtccactca gacaggacag atcatgtcca
     1201 gggcgatcaa tgatacagcc tatttcgaat cattgatcgc ccatgtgatt ccggaaacca
     1261 ttgtcagcct cctcacagtc gcaggcgttt tcgttgtgct cttctccatc aatccaacgc
     1321 tggctctata taccttggcg cccatacccc ttatcatact cggcttcgtc gcctacaacc
     1381 gatacgtccg ccccctattt cgtcacgccc aagcaaagct gggagacctc aacgcggttc
     1441 tccaggacaa tctcagcggg atgcgggaga tccaagcatt cacccaagag gaacgcgaga
     1501 tggaatctgt gggaaaacgc atcatggtgc attcttcggc catcacgaaa gccgtttcaa
     1561 cgagcgcatg cttccacggg ggaatcgact ttttcgcagg cctcggaaca gtctcagtcg
     1621 tcttgttcgg aggcctaatg gcggtgcaag gccaggtatc catcgctgac atcaccgggt
     1681 ttctcctgta tgtgggcctc ttttacgagc ccatcatggg gctcaaccgg ctcaatgaat
     1741 cgcttcaaca agccttggcc gcctccgacc gctatttcga agtcctcgac acacagcccg
     1801 acatagacga tgcccccgac gccgtggagt ttggaagggt cgaaggctgc ataacctacg
     1861 aaaatgtctc cttcaactat gacgaaaccc cggtactcaa gaacataaac cttacgatca
     1921 aacccggcga gatgattgcc cttgtaggcc caacaggcgt agggaaaaca accatggcca
     1981 acttgatacc gaggttctat gatcccacaa gaggacgcgt tctgatcgat ggagtagata
     2041 tccgcaccgc tacaatctcc tctctgcgcc ggaatgtagg catggtgttg caggacgtct
     2101 tcttgtttaa cgggacagta gccgaaaaca tcgcttacgg cagccccaag gctaccttgg
     2161 aggagatcat cgaagcttca atagttgcag gggctgacga gttcattagg gagatgccca
     2221 acgggtatga tacccacata ggcgagcgag gcgtacggct ctccggcggg cagaaacaga
     2281 ggctcgccat agcaagggcc atcctctacg atgcgcctat cctcatactg gatgaagcca
     2341 catcatcagt ggataccgaa acagaagcca agatcgcagc tgccctggat aggcttatgg
     2401 cgggcagaac cacgatagtc atcgcccacc gcctctcgac cattcgccat gccgacagga
     2461 tcatcgtgct agatgaaggc gaaatagtgg aagaaggaac ccatgaggag ctgatggagc
     2521 tcggcggcct ttacgctacg ttagtcatgg ctgacgccag cacagcaggg cttgcgtcct
     2581 gaatggaaca agagcacaca acccctggcc caaaagtctt cgcatgctgc gggagtagta
     2641 gatgacccgg aacttgcagt aatgcgaacc aatgcagcac atccataata tgtctatagt
     2701 atgctttgag gagcttctct atcgtctgat gacacatgaa tcctacatat agttctcagt
     2761 cgcgagcacg tcttctgcgg tctacaacga tatcaactag taagacgccg ctgcgtccaa
     2821 atcgctcgtt tgaaaacgcc tccacgatag cgctacccct ctcccggtgc acatgcatcc
     2881 gggatatgcc tcaggcagat ggctgctccc ataaccccga ggtacagtat gtgctgccat
     2941 ggaagtcggc acacccagcc tattagaggc aagcacgctg aagctatcgc agacgcctgg
     3001 gcaactcggc gcaaaacaag atagagcacc agccacagta cgcccgcgag cagcactacc
     3061 actggtgcag tgagcacaca tgagccgaag aacgtggcta tcgccttccc tcctgcgaat
     3121 ccaaaccaga caggcaggat gtgcccgatc accgcggccg ccccggctgc catctgccc
//
LOCUS       DAOSAI010000304         3151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_19775_length_3151_cov_3.078165, whole genome shotgun sequence.
ACCESSION   DAOSAI010000304 DAOSAI010000000
VERSION     DAOSAI010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19775_length_3151_cov_3.078165"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PLM83_09270"
     CDS             complement(<1..777)
                     /locus_tag="PLM83_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOK71628.1"
                     /translation="MTAQTCRKPLVSAVGVSKHFVMSESLLSRRLTGLSARQVMAVDA
                     VSMEIYPGETVGLVGESGCGKSTFGRVMVGLLQPTSGQVYYGNTPIWGQKSIDRSELK
                     HRAQIIFQNPYSSLNPRYTVRQTIDVALAARGVPPRHRYEEARSLMTRVGLSPDYLER
                     YPHQLSGGQRQRVAIARALATQPEFIVADEPLSSLDVSVQAQIISLLEDLQNEYGLSY
                     LLIAHDLRVVYHMCNRVAVMYGGQIVEFAPTDALYERPLHP"
     regulatory      990..1134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1335..2888
                     /locus_tag="PLM83_09275"
     CDS             1335..2888
                     /locus_tag="PLM83_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK71629.1"
                     /translation="MKSRTILTTLIIAMILAFGFLSTTTGQTRKQLSVVIDTDSTRAD
                     EAQAIAHNLRAIGVSADVRIWEWNALKERIQAGERQMYLTAWGSAYFDPFDIIVPKFY
                     SGDRGNYSGYSNARFDAALDRALRAVDNDARREAYFDAQQILYDDAPAIFGYSLMEVE
                     AARSDVLNWQPSMDSRVNLHDVGLARGDTIVVGLYADKILTLDPAAYRDRPTETVIRN
                     MFDALVTRTWDGKVVPEIAEAWKAVSDTEYLFRIRRGIKFHNGDLLDADDVVFTFDRV
                     LREGAMGGRTSPRKGLLGPVEKVEKVDQYTVRFVLSSPFPVLPQALVHFQIVPRKYVE
                     RVGDEAFARKPVGCGPFKYVEGQFDDKIVMERFDDYYGGSPSIPPVGPARVKRAIFRM
                     MPDPTVRVAAVKTGEVHIIQSVPSHMAAALVRDKNIQVKSVEGTRVYMVEMNCRRPPF
                     DDPRVRRAMNYAVDWDAILRTVYAGNATRLATAFLPSGFGFNQSIVPYKYDPARAKAL
                     LAEAGYSTK"
     gene            2957..>3151
                     /locus_tag="PLM83_09280"
     CDS             2957..>3151
                     /locus_tag="PLM83_09280"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="transports peptides consisting of two or three
                     amino acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HOK71630.1"
                     /translation="MAVAAFIFMRAIPGDPVDIMMGEAAWTSEEEMAALRGEMGLDEP
                     MHVQLWTYLKQVFKWDLGYSI"
BASE COUNT          728 a          781 c          922 g          720 t
ORIGIN      
        1 cggatggagc ggcctttcgt atagagcgtc ggttggggca aactcaacga tttggcctcc
       61 gtacattacc gctaccctgt tgcacatgtg atagaccacg cgcagatcgt gggcgatgag
      121 gaggtatgat aacccgtact cgttctgcaa gtcttcaaga agcgagatta tctgagcctg
      181 caccgacaca tcaagggatg ataggggttc gtcagcgact atgaactcgg gctgagtggc
      241 aagcgccctc gctattgcaa cgcgctggcg ttggccgccg ctgagttgat gaggatagcg
      301 ctcaaggtag tcaggtgaaa gccctacccg ggtcatcaag gaacgtgctt cctcgtagcg
      361 gtggcgggga ggaacgcctc tggcagcaag cgcgacatcg attgtttgac gcacagtata
      421 tctcgggttg agagagctgt aagggttttg gaatatgatc tgggcgcgat gtttcaattc
      481 tgaacgatcg atcgacttct gtccccagat aggggtattg ccataataca cttgccctga
      541 tgttggctgg agaagcccca ccatgacgcg gccaaaagta ctcttgccac agcctgactc
      601 gccaacgaga cctactgttt caccagggta tatctccatg ctcacggcgt ccacggccat
      661 tacttgtctg gcggagaggc ctgtcagcct gcgtgaaagg agtgattcag acattacaaa
      721 atgcttggat acacctaccg ctgaaaccag gggcttgcga caggtctgcg cagtcatcaa
      781 acccacctcc gtgaaagcat tataacatcc ctacgcgatg aaagaagcca atgcacaaaa
      841 gtcttttaag ataattcata caaagttgat ccgcgagagc agcgaatatg tagtttgagc
      901 ccagctgagg agcaatttga caacgtgacg gtttggtggt agcatagatc aaagaagagt
      961 agatcataga gggtatgatc atgcgccgcc aggcgcaatc gctgaatctt cccagcaggg
     1021 gagagcgggg gacccatatc atatggcagg ggcgaatcct gcaggtgtgc aggtagggct
     1081 aattccttca gcccgagccc gtcagctaac ctcgtaagcg ctaagaaggg acgcccattt
     1141 aacccaggga tgtccctggg ttttttgtca tgtcgcctgt atggcgtcgg ccatactgtg
     1201 cgggctcagg ttttaagtcc gcgcttagca ggcatccgca gactgccccc gtttttcccc
     1261 aagataattg catacgtcat gaagcatgat gaatcggttt ttcccaagta agcttgtctt
     1321 aggaggttgg tgtcatgaaa tcacggacta ttctgacaac tctcattata gccatgattc
     1381 tggcttttgg ctttctgtct acaaccacgg gtcaaaccag gaaacaactg tctgtagtaa
     1441 tagatacgga ctcgacccgc gccgatgaag cacaggcgat agctcacaat ctgcgggcca
     1501 ttggggtatc tgccgatgtg cggatatggg agtggaatgc gctcaaggaa aggattcagg
     1561 cgggtgaacg acagatgtac ttgactgcct ggggaagcgc ctatttcgat cctttcgaca
     1621 tcatagtgcc caaattctac agtggcgacc ggggcaacta ctccggttat agcaatgctc
     1681 gtttcgacgc ggccctcgat cgagctcttc gagctgtcga taacgacgcg cgcagggagg
     1741 cgtatttcga tgcccagcag atcctgtacg atgatgctcc ggccatattc ggatactctc
     1801 tgatggaagt cgaagccgca cggtcggacg tgctcaactg gcagccgtca atggattctc
     1861 gcgtcaacct gcacgatgtt gggttggccc ggggcgatac gatcgtagtg ggcctgtacg
     1921 ccgacaagat actgacattg gatcccgctg cctatcgcga cagacccact gaaacggtta
     1981 ttcgcaatat gttcgacgcc ctcgtgacgc gcacatggga tgggaaggta gtcccggaga
     2041 ttgcagaagc gtggaaagcg gtctcggata cagagtatct attcaggatt cgcaggggca
     2101 taaaattcca taatggggac ttgctcgatg ccgatgacgt ggttttcacg tttgaccgag
     2161 tgctgaggga aggcgccatg ggtggccgca catcgccgcg caaaggcctg ttgggtccgg
     2221 tcgaaaaagt ggagaaggta gaccagtata cggtcaggtt tgttctgtca tcgccatttc
     2281 ccgtattgcc gcaggcactg gtccactttc agatagtgcc gcgaaaatac gtggagcgag
     2341 ttggcgatga agcgtttgca agaaagcctg ttggctgtgg accgttcaag tatgtagagg
     2401 ggcaatttga tgacaagatc gtaatggaga ggttcgacga ctactacggc ggatctccct
     2461 ctattcctcc ggtggggccg gctcgggtta agcgggcgat cttcaggatg atgcctgacc
     2521 ccactgtgcg tgtggctgct gtgaagacag gggaggtcca catcatccag tcggttccgt
     2581 cgcacatggc agcagccttg gtccgcgaca agaacatcca ggtcaaatcg gttgagggaa
     2641 cccgtgttta catggtagag atgaattgta ggcgtccgcc ttttgatgat ccaagggttc
     2701 gcagggccat gaattacgca gtggactggg acgcaatttt gaggacagtc tatgccggaa
     2761 acgccacgcg tcttgcaacc gccttcttgc cgagcgggtt tggtttcaac cagagcattg
     2821 ttccgtacaa gtatgatcct gcgcgcgcca aggcgcttct tgcagaggca ggttactcca
     2881 ccaaatgatt cgcctcagtc gcacacaggc caagatagta gagtgaatcc tggccaccat
     2941 accgctggcg ctcggtgtgg cagtagccgc tttcatcttc atgagggcca tccctggtga
     3001 cccagtagac atcatgatgg gggaggcagc gtggacttct gaagaggaaa tggcggctct
     3061 tcgaggggaa atgggactgg atgagcccat gcacgtgcag ctttggacct acttgaagca
     3121 ggttttcaag tgggacctgg ggtattcgat t
//
LOCUS       DAOSAI010000305         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_19809_length_3146_cov_4.158525, whole genome shotgun sequence.
ACCESSION   DAOSAI010000305 DAOSAI010000000
VERSION     DAOSAI010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19809_length_3146_cov_4.158525"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..402
                     /locus_tag="PLM83_09285"
     CDS             178..402
                     /locus_tag="PLM83_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71631.1"
                     /translation="MDRVQHHEVRELIAVFGGDRGGVNQNVAGIFRALKRIWNVLGLL
                     IGHCAQVMHRLVSTLLVSATGEPYAGVGTR"
     gene            787..2451
                     /gene="argS"
                     /locus_tag="PLM83_09290"
     CDS             787..2451
                     /gene="argS"
                     /locus_tag="PLM83_09290"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOK71632.1"
                     /translation="MRSLEGAIRKLVDSNRLCADVLDHLSSIALEVPKDARHGEFATP
                     MALSLAGVARKAPREIASLIVEEMEQEGFSSYVERAEIAGPGFINFHLSSKWLVDTIG
                     LVRSRDEKYGCSDYGRGERILVEFVSANPVGPLNIVNARAAAVGDTLVRLLNASGYSA
                     ASEFYVNDAGRQVSVLGESVYARYMELEGVDYPFPEDGYRGEYVKDVARKAMEEHGDR
                     ISAMDSDDARHFLQEFALNYILDWQRRSLEAYEVRFDRWFSERKLRQTGAVEDMVREM
                     AEAGYTYEKDGAVWFRSSDYGDDKDRVLVKSDGLYTYVAPDLAYHRDKLARGFTKLID
                     ILGPDHHGYEGRMQAGLKAFGHPDGTLEVLILQLVTLMRDGQPVKMSKRAGEFITMDD
                     LLEEVPKDAARYFFIMRNTSSHLDFDLDLAKAQTTDNPVWYIQYAHARCCSIFRQAEA
                     EGIQPTEWEQADVSCLVHPSEIDLVRKMASFPDEVLTAALERAPSRIAKYALELAGLF
                     HSFYTDCRVISDDEAQTRARLALVDAARIVLRNVLGIMGVSAPERM"
     gene            2456..>3146
                     /locus_tag="PLM83_09295"
     CDS             2456..>3146
                     /locus_tag="PLM83_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71633.1"
                     /translation="MERYSPVEAWNVLKKSFRLAYERLGMVVLMSVIWYFATFTTVFI
                     SGPTIWAIPIFAVVAAPLHMAIMYCGNLAAHREDAGLKELWQGYKKFWTRAALLGAIH
                     SAVILILWIDIQVLLGTDSSLMRFLSGVWVYLSIFVGLLFMYAYPIIVEQDTTVWKSI
                     RRAVILILDNPAYTFAVGLLAAVFFAAGVLPTIGILAGNKTAGYFMIIGLSLYAGFAS
                     IYRNLATVRALQ"
BASE COUNT          718 a          717 c          943 g          768 t
ORIGIN      
        1 cgctcgcgct cagcggctat ggttttgtag aacgatctgc attgagcatg caggggattc
       61 cgaggtgcag gccaattcaa acagtcctgc tttccttttc ggatcaaccg ctctatttaa
      121 aacgagaagc tctgcatatc gtattccgcc tggcaactgg tggagagcaa cggagatatg
      181 gatcgagtgc agcaccatga ggtgcgggaa ttgatagccg tttttggcgg agatcgtgga
      241 ggggtcaacc aaaatgtagc tgggattttt cgtgctttga agagaatttg gaatgtcttg
      301 ggtcttctca tcggtcactg tgcccaggta atgcaccggc ttgtgagcac gcttctcgtt
      361 tcggctacag gagaaccgta tgccggagta ggcacgcgct gaccgatatg acagctatca
      421 ggtgagctaa tctcctgtaa atcgggtttt tgagagatct cagctccaaa cgtacatact
      481 ttgtgaggct tcctcgtgcg cttcctagct cgcattaatg aacgttcaat tcttctggtc
      541 tgcaaaccat gtttgaatgg gcgcaaatat agtctacaat atagtttggt gtctattcag
      601 gagcagcctg aaactcgctc gattggatca tggttgtgag ctcgttgaaa tttggggcag
      661 agccgcgtca gatggccagc atgagctagg aaggattctg gcggcgcttc gaggaatact
      721 ggtcggagaa ggcagggaga ggacgtacaa atggacaaca taatggaaca catacgagga
      781 agcatcatgc gatctcttga aggggccatc cgtaaactgg tcgattccaa tcgcctttgc
      841 gcggatgtac tggaccacct gtccagcatt gcgctggagg ttcccaaaga tgccagacac
      901 ggggagttcg ctactccgat ggctctgtcc cttgcgggcg ttgccaggaa agcgccacgg
      961 gagattgcat cgctgatcgt agaagagatg gagcaagaag ggttcagctc atatgttgag
     1021 agggcggaga ttgcggggcc gggctttatc aatttccacc tttcatccaa gtggcttgtg
     1081 gatacaatcg gcctggtgag gtcgagggat gagaagtatg gttgcagcga ctatggacga
     1141 ggcgaacgca ttttggtgga atttgtaagc gcaaacccgg tggggccgct taacatcgtc
     1201 aacgcgaggg ctgccgcggt aggtgatacc ctggtcaggc tccttaacgc aagtggttac
     1261 agcgcggcaa gcgagttcta tgtgaatgac gcgggccgtc aggtgtcggt gcttggggag
     1321 tcggtatatg cgcgttacat ggagcttgag ggagtggatt atcccttccc ggaggatggg
     1381 tatcgcgggg agtacgtcaa ggatgttgct aggaaggcta tggaggaaca tggtgatcga
     1441 atctccgcga tggattcgga tgacgctcgc cattttctac aagagttcgc gctcaactac
     1501 atcctggatt ggcagaggag gtcgctcgag gcctacgagg tccgtttcga cagatggttt
     1561 tcggagcgca agctaaggca gactggcgct gttgaggata tggttcgcga gatggcggaa
     1621 gcgggataca cctatgagaa agatggcgca gtctggttca gatcatctga ttatggcgac
     1681 gacaaggatc gggtactggt caaaagcgac ggcctatata cctatgtcgc gccggatctc
     1741 gcatatcaca gagacaagct ggcgcggggc tttacgaagc tcatagacat actcggtcct
     1801 gaccaccacg ggtacgaagg tagaatgcag gcggggttga aggcctttgg acatccagat
     1861 ggcaccttgg aagtactcat tcttcaacta gttacgttaa tgcgagacgg gcaacctgta
     1921 aagatgtcca agcgcgcggg cgagtttatc accatggatg acctgctgga agaggtgccg
     1981 aaggacgccg cccgttattt cttcataatg agaaacacct cttcgcatct cgatttcgac
     2041 ctcgaccttg cgaaggccca aactacggac aatccggtgt ggtacattca gtacgctcat
     2101 gcgcgctgct gcagcatctt cagacaggca gaggccgagg gaatccagcc tacggagtgg
     2161 gagcaggcag atgtgtcgtg tcttgttcac ccaagcgaga tcgacctcgt ccgcaagatg
     2221 gcgagctttc cagatgaagt actcactgcc gcgcttgaac gggcgcccag ccggattgca
     2281 aagtacgcct tggagctggc agggttgttt cacagcttct acactgactg cagagtgatc
     2341 agtgatgatg aagcccagac ccgggcaagg cttgcgctgg ttgatgctgc aagaatcgtg
     2401 ctccgaaacg tgctggggat catgggagta tctgctccgg agaggatgta gggtgatgga
     2461 gagatactcg cctgtagaag cctggaacgt gctgaaaaag agttttcgtc ttgcctacga
     2521 gcgcctcggc atggtagtgc tcatgagcgt gatatggtac tttgccacat ttaccactgt
     2581 ttttatttcg ggccccacaa tatgggcgat tccaatcttt gctgtcgttg ctgctccact
     2641 tcacatggcg ataatgtact gtggaaacct ggcggcgcat cgcgaagacg caggattgaa
     2701 agaactgtgg cagggttaca agaagttttg gacaagggcg gctttgcttg gagcgataca
     2761 ctcggctgtc attctgattc tatggataga catacaggtg cttcttggga cggacagttc
     2821 tttgatgaga ttcttgtcgg gggtctgggt atatctcagc atttttgttg gacttctgtt
     2881 catgtacgcc taccccataa tagtggaaca ggacacgact gtttggaaat cgatacgtag
     2941 ggcagtgata ctgatacttg ataacccagc gtacacgttc gctgtgggac tgttggcggc
     3001 cgttttcttc gctgcgggtg tcttgccgac catagggatc ctggccggca acaagactgc
     3061 aggctatttc atgataattg gcttatccct ttatgctgga tttgcttcga tttaccgaaa
     3121 ccttgctaca gtaagagcgc tccaga
//
LOCUS       DAOSAI010000306         3131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_19939_length_3131_cov_3.565020, whole genome shotgun sequence.
ACCESSION   DAOSAI010000306 DAOSAI010000000
VERSION     DAOSAI010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19939_length_3131_cov_3.565020"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2194
                     /locus_tag="PLM83_09300"
     CDS             <1..2194
                     /locus_tag="PLM83_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71634.1"
                     /translation="DNHRRVMLVRDSLSQPSVRVVDFDTGMGTEIAVPIAEAMGLSET
                     DLACSDDGTAECGAWTVPVRLLMGDNGRRYERAISLLDGISSDSGAENAYFELRREVE
                     RVVDLRIKRAELRARIAWLVERLSFLDSRISSIDAALRTKVECDEIEEYVGFLKQEFE
                     SWRINLADVLVTEKAAEKARNRLLKYSGMEGAFDPEVAGEIRNLMSEMSELEKTAISY
                     ESEQSGITSAINGLRMELALANKDLEELEQRGLSRRTLEHAQSSLKEIEDRETRLASL
                     SQRIAEVDKVLGRKRLADGLSVGGALLTVTSLTGLLLPENIFPWMSLSEPLMGVLILL
                     LSTGLMCTVYGVSTGIELDAKHGAKVRLEHDRANLSSQVAWHRRRLDSLLHDMSINDY
                     ALAVEKMEWLKEKKECLLEEIKTLSGELARLRNEADLNRVRTWRAKERMGELMKVSGF
                     SSPSMYLEAYEDYRRVEASYREAADRLEAALGGRSRESIERDIEIVLEEIASAEAQRA
                     AIADPRIEEKIWELSEEYAIKADERERLAFELEQKSLELADTEHALAGVDIWEVSSEA
                     ARAASELERARREGLAASLAQAAIEEMIEEKRAQAGARLAEVASDIMAFFTGEPTAEI
                     SCAVEEDCVRCSFPRAADSESLIPVAVLAVRLGASEVLYGRGALPLIIDYSSKGKGGR
                     VGHKSAYQGLTLVWPALEKLGRTRQVILVLDGLEFAEQIPKSCEVLTI"
BASE COUNT          773 a          632 c         1005 g          721 t
ORIGIN      
        1 tgacaatcac aggcgtgtca tgcttgtgcg cgattcttta tcgcaaccgt cagtgagggt
       61 tgtggatttc gatacaggca tgggcacaga gattgctgtc cccattgccg aagccatggg
      121 cctttccgaa accgatctgg cttgcagcga tgatgggacg gctgagtgtg gggcgtggac
      181 ggtgcctgtc agattgctca tgggcgataa tggcaggcga tacgagaggg ctatttccct
      241 tctggatgga ataagcagcg attcaggagc ggaaaatgcc tattttgaac tcaggcgcga
      301 ggtggagcgc gttgtggacc tgcgcataaa aagggcggaa ctgagagcca gaatcgcttg
      361 gcttgttgag aggttatcct ttcttgattc gaggatatcc tccatagatg cggctctacg
      421 gaccaaggtg gaatgcgatg aaatcgagga gtatgtgggg ttcttgaaac aggagttcga
      481 atcatggcgg atcaatctcg cggacgtgtt ggtgacggag aaagctgcgg aaaaggccag
      541 aaacaggttg ctcaagtatt ctggtatgga aggggctttt gaccctgagg ttgcgggcga
      601 gataaggaat ctgatgtctg aaatgtctga gctggagaag acagcgatat cgtacgaatc
      661 agaacagtcg ggtattacgt ctgcaataaa tgggctcaga atggagcttg ccctggcgaa
      721 caaagacctc gaggagctgg aacaaagagg attgtcccgc aggacgctgg agcatgcgca
      781 atcttccttg aaagagatcg aagacaggga gactcgtctt gcaagtctgt cgcaacgaat
      841 tgcagaggtt gacaaggtcc ttgggcgcaa gagactcgcc gatggccttt ctgtgggagg
      901 ggcactcttg accgtcacgt ctctcactgg gcttctcctt ccagagaata tctttccgtg
      961 gatgagcttg tctgagccgc tgatgggggt cttgattttg ctgctttcca ctggcttgat
     1021 gtgcacggtc tatggggttt cgacggggat agagctggat gccaaacatg gtgcaaaggt
     1081 gagactggag cacgatcggg ccaatctttc gagccaggtg gcatggcaca gaaggagact
     1141 cgattcgttg ctgcacgaca tgtccataaa cgactatgcg ctggccgtgg agaagatgga
     1201 gtggcttaag gagaaaaaag agtgtctgct ggaggagatc aaaactctgt caggtgaact
     1261 tgctcgattg aggaacgagg cggacttgaa tcgggtcagg acatggcggg ctaaggagcg
     1321 catgggcgag ttgatgaagg tgtctggttt ttcttctcct tcgatgtatc ttgaggcata
     1381 cgaggactat cgaagggttg aagcatccta cagagaggct gccgacaggc tagaggctgc
     1441 actgggcggg cgttctcggg aaagtataga gcgtgacatc gagatcgtgc ttgaagagat
     1501 tgcttctgca gaggctcagc gcgcagccat tgccgatccc aggatcgagg aaaagatctg
     1561 ggagctgagc gaggaatacg ccataaaggc agacgagcgg gagcggttgg ctttcgaact
     1621 ggagcaaaag tctttggaac ttgcagatac agaacatgcg ttggcaggtg tagacatttg
     1681 ggaggtatcg agtgaggcag cgcgagccgc ctcggaattg gaacgtgcga ggcgtgaggg
     1741 gctcgcggct agcttagcac aggcagccat tgaggagatg atcgaagaaa aacgggcgca
     1801 agctggggca agattagcgg aggttgcctc ggatatcatg gcatttttta cgggcgagcc
     1861 tacggcagag atctcttgtg cggttgagga agactgcgtc agatgctctt tcccgagggc
     1921 agctgactcg gagtcgttga ttcctgtggc tgttctcgct gttcggcttg gcgccagcga
     1981 ggttctatac ggaagaggcg ctctcccttt gataattgac tattcttcta agggtaaagg
     2041 cggacgggta gggcacaagt cggcatacca agggttaacc cttgtttggc ccgctctgga
     2101 gaaacttgga cgaacaaggc aggtaatatt agtattggac ggattggagt tcgcagagca
     2161 gataccaaaa tcatgtgaag ttttgacgat atgagggatt gctaagggtt cgcgtttgtt
     2221 gtggtcggga cggaggagat caaagaacga cgagccgatg ttcgaatatg tgacttcgga
     2281 atgcaaatgc gaacgggaag cgatgcggta gcatcgctct cagtgttttt gggtctgagt
     2341 atcttagaag aagtatgtga gtgctttcca gacaagtctt ctgtttggca atctaaaagt
     2401 tcatatgttg accaccacgc ttaggaccga aattcggggc ctgcaattgg aagagggcat
     2461 gctgtagacg gcagctttcg cctgtgaatg caagcatgtg cccatgatga acaagtctgt
     2521 cgatgagcga ggcggttcct atcatggctg cttttggaca tgctttttga ggacatggcg
     2581 agtttttcga caagggtcgg ccagaaaatc ggcacaacgc tggaatgata ggctaaatga
     2641 gtgatagcaa gcatggacag gaatgcaggg cagttcagag attgatcaga agttacttgg
     2701 agaagaaaac ttcgtttttg tacggtttcc cagaaatcca cagtgctgca ggggcttctg
     2761 tacgtctaac aagctttgcc ttgggaggga tgacgccggc acagaagttg cagcgaagaa
     2821 ggggacatct agcgcaatcc acaacgctcc tagcccactt tagcaatatc ggcaaaaaac
     2881 ggtttggttg ctgtatgcgc cttgcattct ggtaatgcca atatcaatgt aggactggtc
     2941 ggaatggaac tcccatccca caaaggacga aagaagtcaa gaaattgcat gggcctatgc
     3001 aggtgtatgg ggcgtggaca gacaatagag gccatagaac cagtggcagt aggaggtcag
     3061 gacgatgggg gcggacttga tcttaggaaa tggagttgtg gtggactgcg gataccctgc
     3121 acgggtgata g
//
LOCUS       DAOSAI010000307         3091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_20301_length_3091_cov_4.074440, whole genome shotgun sequence.
ACCESSION   DAOSAI010000307 DAOSAI010000000
VERSION     DAOSAI010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20301_length_3091_cov_4.074440"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..392)
                     /gene="yabP"
                     /locus_tag="PLM83_09305"
     CDS             complement(81..392)
                     /gene="yabP"
                     /locus_tag="PLM83_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HOK71635.1"
                     /translation="MAAKGPDLSDEARRKRPGLQHRVVLVDRESMTVDGVANVESFDD
                     EEIILETNAGALTVTGHDLHIRQLNLEDGTIEIEGYVETCDYFDESPKKGRGLLGKLL
                     R"
     gene            complement(511..1401)
                     /gene="mazG"
                     /locus_tag="PLM83_09310"
     CDS             complement(511..1401)
                     /gene="mazG"
                     /locus_tag="PLM83_09310"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOK71636.1"
                     /translation="MADIEEIDRLQRLEAAQRTVKSDAASGAEVLGESQCRYPADKLV
                     EIMSRLRGEDGCPWDRKQTSSSLKPYVIEEAYEVVEAIDRQDPNLLCEELGDLLLQVV
                     FHSQIAREEGAFDFNDVVEAICAKLVRRHPHVFGDVEADDAGTVLRNWERIKQKEKES
                     SNGAPASILDEVPRYLPALMQAVKIQDKASRVGFDWECPEDAAAKVWEETEELREAMA
                     DGEMAKVEEELGDLLFAVVNVARLMKIDPEEALRRTNSKFASRFKFIEHRADENGVAL
                     EEMTLAEMDAIWDEAKEKGL"
     gene            complement(1432..>3091)
                     /locus_tag="PLM83_09315"
     CDS             complement(1432..>3091)
                     /locus_tag="PLM83_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOK71637.1"
                     /translation="RDKTAFLRGAAIMAAAGVISRILGAVYRIPLAHTIGDEGMGLLE
                     LAQPLYNFFLVASVAGLPLAVSKLVAERIAVGDERGSFRILRVAILIMLCTGLASSSL
                     MYFGADWFAERVYHDVRVALAMRAIAPALFFVTVMSAFRGYFQGCQDMGPSAASQVVE
                     QLVRVGTMLTLAAALMPRGVEYAAAGAALGAVTGGMAGLAYLGIAFARRMSAGGVGRG
                     KSARSKKAARACIRTGVGNRATKEETTWELVADIFSIALPVAIGASVLPIVRAIDSAM
                     VPARLGAAGFTAETATALFGQLSGMALPLAYFPNVLTIALATSIVPAISESLVAGSRS
                     RALRRAGSAIRLTALISLPAAFGLWTLSSEICSLVYGRPEVGRVLAALAPASAFLCVL
                     QTSGAILQGMGYPGAPVKNLVAGSVVKVVCEYWLTGRPRLNVVGAAISTSVGFGVVAL
                     ANLADVARRLGHGAGVRLASEFVKPAISAAIMAVVARTAYSFLVRATVREGLATIASM
                     MVAAVAYFLVMLVIGGIVGADIEMIPKIGPPLARVLGRIGLVKS"
BASE COUNT          701 a         1038 c          709 g          643 t
ORIGIN      
        1 ctattacact cttcgccccg tcccgatgcc cctccaacca cgttcccaaa gggccaatta
       61 atctggtaga tcagcccagc ctatcggagc aatttgccca gcaagcctct ccccttcttc
      121 gggctctcgt cgaaatagtc gcatgtctca acatacccct cgatctcgat cgttccgtcc
      181 tcgagattga gctgccttat gtggagatca tgcccggtca cagtgagcgc tcccgcattc
      241 gtctccagaa ttatctcctc gtcatcgaag ctctccacat tagctacccc atccaccgtc
      301 atgctttcac gatcaaccag cacaaccctg tgttgcaagc ccggacgctt ccgccttgct
      361 tcatcagaca ggtcaggtcc tttggcggcc atcggcacca ctccttcgga caaatctttg
      421 catcgatagc gcgcatctct catggagcac atcaatttgt cctatggatg tctatgccca
      481 ttcccaccat ataatgcatc aactcgaaac ctaaaggccc ttctccttcg cttcatccca
      541 gatagcatcc atctccgcga gagtcatttc ttcgagagcc actccgttct catcggccct
      601 gtgttcgatg aacttgaacc tcgacgcgaa cttcgagttg gtacgcctca gagcctcctc
      661 cggatctatc ttcatgagcc tggccacgtt aacaacagcg aacagaagat cccccagttc
      721 ctcctcgacc tttgccattt ccccatctgc catggcctcg cggagctctt ccgtctcctc
      781 ccaaaccttt gcggctgcgt cctccggaca ttcccaatca aaccctaccc tgctcgcctt
      841 gtcctggatc ttgaccgcct gcatgagagc gggcagatac ctaggaacct catcaagaat
      901 cgaagcgggc gccccgttgg acgactcctt ctccttctgc tttatccgct cccagttacg
      961 caagacagtg ccggcatcgt ccgcttccac atcaccaaac acatgtgggt ggcgacgcac
     1021 cagtttggcg cagatagcct ccacaacatc attgaaatcg aaggccccct cttcgcgggc
     1081 gatctgggaa tgaaagacca cctgcaggag caggtcgccc agttcttcgc agagaagatt
     1141 cggatcttgc ctatcgatcg cctccacaac ttcgtatgct tcttctataa catacggttt
     1201 taaggaagac gacgtctgct tgcgatccca aggacatccg tcctcacccc ggagcctgga
     1261 catgatttct acgagcttgt ccgccgggta tctgcattga ctctcaccta atacttcagc
     1321 ccctgaagcc gcatcggatt tcaccgtcct ttgggctgct tcaagcctct gcaatctatc
     1381 gatctcttcg atgtctgcca tgtctaccac accttccgca gtaattcgtc actacgactt
     1441 gaccaaaccg atccgcccca ggacacgcgc gaggggcggt cctatcttag ggatcatttc
     1501 gatgtcggca ccgacaatcc cgccgatgac gagcatcacc aaaaagtagg ctacagctgc
     1561 caccatcatt gatgcgattg tggcaagccc ttcccttaca gtggcgcgga cgaggaatga
     1621 gtaagcagtc ctggctacca ccgccattat cgcagcggat atcgcgggtt ttacaaactc
     1681 cgaggcaagg cgaacccctg cgccatgacc aagccttctt gcaacatcag ccaggtttgc
     1741 cagggcaact accccgaatc ccacagaggt gcttatcgcg gctcctacca cgttcagccg
     1801 cgggcgacct gtaagccagt attcacatac cacctttaca accgaaccgg ccaccaagtt
     1861 tttgactggc gcccccggat aacccatgcc ctgtaaaatc gcgcctgatg tctgcaatac
     1921 gcacaaaaaa gccgaagccg gagcaagcgc cgcaagcacc cgcccaacct caggtcttcc
     1981 atagaccaga ctacatatct cagagctgag tgtccagagc ccgaacgctg caggaaggga
     2041 tatgagcgct gtcagcctaa ttgcgctccc tgcccgcctc aaagccctcg atctgctccc
     2101 agccaccagg gattcggaga tcgccggcac gatgctagtt gccagtgcaa tcgtgagaac
     2161 gttagggaag tatgccagtg gtagtgccat cccgctcaac tgtccgaata gggccgtagc
     2221 tgtttcggcc gtaaagcccg cagccccaag ccgcgccgga accatcgccg aatcgatcgc
     2281 tctgacaatc ggtaacaccg aagcgccgat ggccaccggc agcgcaatcg agaagatgtc
     2341 ggccactaac tcccatgttg tctcttcctt tgtcgcccgg tttcccacgc ctgtgcggat
     2401 acatgcgcgc gcggccttct tgctcctggc cgatttgcct cttccaaccc ctccggcgct
     2461 catcctccgg gcaaaggcga tccccaagta agcgaggcca gccatcccgc ctgtaacagc
     2521 tcccagcgcg gcgccagcag cggcgtactc caccccgcgg ggcatcaacg ctgcagccaa
     2581 agtcagcatg gtacctacgc gcacaagctg ctccacaacc tgagatgcag cggacggccc
     2641 catgtcttga cacccctgga aatacccgcg aaaagcggac atcaccgtga caaagaaaag
     2701 agcgggagca attgccctca tggcaagagc tacccgcaca tcatggtaga cacgttctgc
     2761 aaaccagtca gccccgaaat acatcagtga cgaagatgca agcccagtgc atagcattat
     2821 gagaattgca accctcagta tgcggaacga tccgcgctca tccccgactg caatgcgttc
     2881 agctacaagc tttgacacgg caagaggcag ccctgccaca gaggcgacaa gaaagaaatt
     2941 atataagggt tgcgcaagct cgagcaaccc cataccttca tcgccgatag tatgagcgag
     3001 agggattcga tagacagcgc cgagtatccg gctgatcact ccggcagccg ccataatggc
     3061 ggccccccgg agaaatgccg tcttatcccg g
//
LOCUS       DAOSAI010000308         3069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_20503_length_3069_cov_3.694426, whole genome shotgun sequence.
ACCESSION   DAOSAI010000308 DAOSAI010000000
VERSION     DAOSAI010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20503_length_3069_cov_3.694426"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..2352
                     /gene="metG"
                     /locus_tag="PLM83_09320"
     CDS             289..2352
                     /gene="metG"
                     /locus_tag="PLM83_09320"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOK71638.1"
                     /translation="MTKEPFFVTTPIYYVNDMPHLGHSYTTIGCDALARYKRMCGYDV
                     FFLTGVDEHGQKIERRATAEGKSPQEYVDYVTDAFKRLWAELDISNDGFIRTTDPEHV
                     RAVQHVFQQLYDSGDIYKGKYEGWYCTPCEAFFMESQLVDGNCPDCGRPVEWLQEESY
                     FFRLSKYEDRLLAHIEANPDFIQPATRRHEMVNFIKSGLNDLCVSRTTFDWGVPVPFA
                     PGHVVYVWLDALINYLTASGYVDDPERFAKYWPADVHVVGKEIVRFHSVIWPAVLMSL
                     GLPLPKKVFGHGWWTVEGTKMSKSVGNVVKPHDYAKEFGVDAVRYFVLREVPFGQDGD
                     FSRASFVYRVNADLANDLGNLLSRTTAMINKFAGGKVPEPGEWDDADKKIPEIATDAV
                     DKAARAFDDLAFSEALENVFRLVDAGNKYIDDVEPWNLAKTDAGRRRLGTVLYNLAES
                     LRIAAVALSPVLTSKSAEIWYQLGIDGDPAEAGWEATKWGGTVPGTEVRRGDPIFPRI
                     DVKAALKEEASSESGSASSREGSGGRVKGESVQCCGGDFGEGHAQPDGGKAVLRAAAA
                     PAREGEALDADNIISIEDFAKIDLKVGTVLEAERIEGADKLLVLQVDIGSETRQIVAG
                     IAKHYSPEELIGKQIVVVTNLKPAKLRGVMSNGMLLAASTTGDPKEVRVVTPDGPVPA
                     GSRVK"
     gene            2356..>3069
                     /locus_tag="PLM83_09325"
     CDS             2356..>3069
                     /locus_tag="PLM83_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOK71639.1"
                     /translation="MIIDSHAHLEDEAFVEGVYAVMERARAAGVEIVINPGADVRSSR
                     VAAENAAVHEGLYAMAGIHPHDAAGVGDGDLEEISRILSMPRVVGIGEIGLDYYRDLS
                     PRDEQIRVFRAQLELAKEFGCPVQIHSRDAHRDTFEILKEYANDVPAIVLHCYSGSPE
                     MAAELIELGFFISLGGPVTYRNARKSVEVAKSVPLERLLVETDSPYLPPSPYRGERNE
                     PAYVVEVVRKIAEIRGVSTE"
BASE COUNT          692 a          727 c          949 g          701 t
ORIGIN      
        1 ccaagaagag aagcgcgaat ttcgtgcaat tgttcggttt gcgatcgatt ttggatggac
       61 attctcgaaa caaccccgca tagctggaca atcttttcac ctcgctatta aaatacctca
      121 taatgggctg gtgttcaaca gtaaggcatt ccttgacagt tcacggtggc tcacggtata
      181 ttcaacttag cctttaccag gcatgctagc ttttgttttg ccccctgtgt gtgtggcgga
      241 aactcagcgg cagagcagag gttcttgtct attttggagg gactgtttat gacaaaggaa
      301 cccttttttg ttactacacc tatatactac gtgaacgaca tgccccacct tggccattcg
      361 tatacgacga tcggctgtga cgcgttggct agatacaagc gaatgtgcgg gtatgacgta
      421 tttttcctca cgggcgtcga cgaacatggg cagaagatcg aaaggcgcgc caccgcggaa
      481 gggaagagtc cccaggagta tgtggactat gtgacggacg cctttaagag actctgggcg
      541 gagcttgaca tatccaatga cggcttcatc cgcacaacag atccggagca cgtgcgggcg
      601 gttcaacacg tatttcagca actgtacgat tctggagaca tctataaagg gaaatatgaa
      661 ggatggtatt gcacgccgtg cgaggcattt ttcatggagt cgcagcttgt tgacggcaat
      721 tgtcctgact gcgggcgacc tgtggaatgg ctgcaggagg agagttattt cttccgtttg
      781 tcgaaatatg aggaccggct tctcgcgcac atagaggcta atcccgattt catacagcct
      841 gcaacgaggc gccatgagat ggtcaatttc ataaagagcg gattgaacga tctttgcgtg
      901 tcgcgaacca ctttcgattg gggtgttcct gtgccttttg cgccgggaca cgtcgtctac
      961 gtttggctgg atgccctcat caactatttg accgcgtcgg gctatgttga tgatcccgag
     1021 aggttcgcca agtattggcc tgctgacgtt cacgttgttg gcaaggaaat agtcaggttc
     1081 cattctgtga tatggcctgc ggtgctgatg tctttgggat tgcctctccc gaaaaaggta
     1141 tttggccatg gatggtggac ggtggaaggg acgaagatgt cgaaatccgt gggaaacgtg
     1201 gtaaagccgc atgactatgc gaaggagttt ggagtggacg cagttcgcta cttcgtcctc
     1261 cgcgaggttc cgtttgggca agacggggat ttctcccgcg catcctttgt atacagggtc
     1321 aatgctgacc tcgcaaacga tctcggaaat ctgctcagcc gcaccacggc gatgataaac
     1381 aagtttgccg gtggaaaagt gcctgaaccg ggagaatggg atgacgctga taagaagatc
     1441 cccgaaatcg ctaccgacgc tgtcgacaag gctgcccggg ccttcgatga tttggcgttc
     1501 agtgaagcct tggagaatgt attcaggctc gttgacgcag gaaacaagta catcgacgac
     1561 gttgagccat ggaatcttgc caaaactgat gctggccgca ggaggctggg cactgtcctt
     1621 tacaacctgg ccgaaagcct aaggattgcg gccgtggctt tatcacccgt gctaacaagc
     1681 aagtcagccg aaatctggta tcagctgggt attgacggag acccggcgga ggcgggatgg
     1741 gaggccacca agtggggcgg cacagttccg ggcaccgagg ttagacgggg tgacccgatt
     1801 ttccctagga ttgatgtgaa ggcggcattg aaggaggagg cgtcgtcaga gagcgggagc
     1861 gcatcctcgc gcgaaggctc tggtggcaga gtgaagggtg agtcagtcca atgctgcggt
     1921 ggggacttcg gcgaagggca cgcgcaaccg gatggcggga aggctgtttt aagggctgca
     1981 gctgcgccgg ctcgcgaggg tgaagcgttg gacgccgaca acatcatatc catagaggac
     2041 ttcgcaaaga tcgatctcaa ggtaggtact gtgctcgagg ccgagcggat cgagggcgcc
     2101 gacaagcttc tcgtgcttca ggtggacatc ggctccgaga ccaggcagat agttgcgggc
     2161 attgcgaagc attactctcc tgaggagctc ataggcaagc agatcgtggt tgtgactaat
     2221 ctgaagcctg cgaaactgcg cggcgtcatg tcaaatggaa tgctgcttgc ggcatcgacc
     2281 acaggtgatc ccaaggaggt tagggttgtg accccggatg gcccagtgcc cgctggaagc
     2341 agggtgaaat aggcgttgat tatcgattcc catgcccatc ttgaagatga ggcttttgta
     2401 gaaggcgtgt acgccgtgat ggagagggcg cgcgcagccg gcgtcgagat agtaatcaat
     2461 cctggcgcgg atgtgcgctc gtcccgtgtg gctgctgaga atgcggccgt acatgaagga
     2521 ctttacgcca tggccgggat ccacccccat gatgcggccg gagtgggcga tggtgatctg
     2581 gaagagatat cgaggatcct ttcgatgccc cgtgtagtgg ggattggcga gatagggctc
     2641 gactattata gagacctttc gccgagagac gagcagatca gggtctttcg agcccagctc
     2701 gagctcgcga aggagtttgg atgcccggtt cagatccata gccgcgatgc ccatcgtgac
     2761 accttcgaga tcctgaagga gtacgcaaat gatgtgcctg ccatagtgct gcattgctac
     2821 tcgggcagcc ctgagatggc tgcggaactg atcgaactcg ggtttttcat atcactgggc
     2881 ggtcctgtca cctacaggaa tgctcgaaag tcagtggaag ttgcgaaaag tgtgccgctt
     2941 gagcgtctcc tggtggagac tgattcgccg tatcttcctc caagccctta ccggggcgag
     3001 cgaaacgaac ctgcttatgt agtggaagtt gtacggaaga ttgccgaaat caggggcgta
     3061 tcgacggaa
//
LOCUS       DAOSAI010000309         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_20601_length_3058_cov_4.052947, whole genome shotgun sequence.
ACCESSION   DAOSAI010000309 DAOSAI010000000
VERSION     DAOSAI010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20601_length_3058_cov_4.052947"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="PLM83_09330"
     CDS             <1..1501
                     /locus_tag="PLM83_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOK71640.1"
                     /translation="MDIWQPLFPRMSTTKVQARLTCLGFDPGPCDGKYGPFTRDAVRQ
                     CQAAYRLVADGVLGPEIKRLLSQDLPSCRVTVRAVEGQSLSGVARALGTTVEALVEGN
                     RRKRYEDVFPGERLMVHKRAVGAFMYPLAGAEGGTVDLDITFELPWTFALFPMFAIAD
                     TGEVRSVMSPSPLAVGLTERENGRVDSIRVSRSLRGRKQGAEPLAIPVAKKSHADYGS
                     PVGSSVQFLPGARESAAAAKTVRSSGGTQFFAMLSGPADINCGTLALLRSGRAAIVST
                     TCSIAREAAASGAAGVAVNIPGLGDDECSMYSRFVRCLSRECHRLGMKLAVIIPAPSW
                     QADGQVHFQGSSAPERRLPWPNGYDVELLGSLADLVLLDIADPSAFWDSIVLACRHVP
                     RWKLAAVLELRAYRQEPDGHVPMSQEELRAFMARHVVRGGRDEFSRVPYLSYRARGVV
                     RRIWQEDAESLGKKLHAVNRLNILGVVFKGARDAREQVLDEVCRRFIIM"
     gene            1563..>3058
                     /locus_tag="PLM83_09335"
     CDS             1563..>3058
                     /locus_tag="PLM83_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HOK71641.1"
                     /translation="MGIQLEHRQLSITRVAAQGMAEISLQRSVALLQDHPDISDDSAH
                     SILWAQGVPIVASVEPMDGQIKVTGYVQSTVFYVAKGAPDFVGKAVMSEPRFEAVIPA
                     PGATVSDRVKVDLTLSQLEVETTGTRGLLLAATIMASARALRDEVVEAAVSAEATGGS
                     RISVHSEEINVTSIHESFSEKVEFSEILSIPDEKAACLGSESACGAAGTARVIGIQVG
                     DGKVTLDAEISVEIASTVVRGVPALNILRFERVPIHASFDVPAAEKGMTALTQLALSH
                     VSAILLSDAEVSIDGILDVDVDLVSSERLSVIVDIDSETQEIVDIDTAGITVEKLVGE
                     GMIDLDLHDSVALQALMRRYSFTGMEDVEGSIGLLGLSDIETTDDEVSLRGLLTARAI
                     LSDVDEDEEGQFPINFALDIPIEFSDSAEVPGVRAGDRAEPRSATHTVVVGKSAPNRL
                     DVEGSLRVDVEVYRPVELQVVTSAETISPVTLDPYAMTFYVVGPGDTLA"
BASE COUNT          635 a          780 c          966 g          677 t
ORIGIN      
        1 catggatata tggcaaccac tttttcctcg catgagcacc acaaaggttc aagccaggct
       61 gacgtgcctg ggatttgacc ccggcccctg cgacggaaag tatgggccct ttaccaggga
      121 cgcggtgcgc caatgccagg cagcctacag gctcgtggcc gacggcgtcc tggggcccga
      181 gattaaaagg ctgctcagcc aggatttgcc ttcatgccgg gttacggtga gggctgtaga
      241 aggccagtcc ctatcgggtg tagctcgagc tctgggaacc accgttgagg ctttggtaga
      301 gggcaaccgg cgcaaacgct atgaagatgt gtttccaggc gaaaggctca tggttcacaa
      361 acgagctgta ggcgcgttca tgtatccttt ggctggtgcc gaggggggca ctgtggatct
      421 ggacatcact ttcgaactgc cgtggacttt tgccctattc cccatgttcg ccatcgccga
      481 caccggcgag gtgcgctcgg ttatgagtcc ttcgccgctc gcggtgggcc ttaccgaacg
      541 agaaaatggc cgcgtggact ctatcagggt atccaggtct ttgcgcggga ggaagcaggg
      601 ggcagagcct ttagctattc cagttgccaa gaaaagtcac gcagattatg gatcgcccgt
      661 aggatcatcc gttcagtttc tacccggagc ccgtgagtct gcagccgccg cgaagacggt
      721 gcggagctcc gggggcactc agttctttgc tatgctttcg ggccccgctg atatcaactg
      781 tggaaccttg gcattgctca gaagcggccg ggcggccata gtttccacca cttgctccat
      841 tgccagggaa gcagccgcat cgggtgcggc gggggtagcc gtaaacatcc ctgggctcgg
      901 cgacgatgag tgttccatgt attcgcggtt tgttcgttgt ctttcgcggg aatgccacag
      961 gctagggatg aagcttgcgg taatcatccc tgcgccgtct tggcaggctg atggccaagt
     1021 ccattttcag ggctcttctg cgcccgaaag gcgcttgccg tggcctaatg gatacgatgt
     1081 agagctgctc gggtccctcg cagatctggt attgctcgat attgcggacc catctgcgtt
     1141 ttgggacagt attgtgttgg catgtaggca tgtccccaga tggaaactgg cggctgtgct
     1201 ggagcttcgg gcatatcggc aggaaccgga cggacatgtg cccatgagcc aagaggagct
     1261 aagggcgttc atggccagac acgtagtgag aggcggaagg gatgagttta gccgggtccc
     1321 gtatctatcc taccgcgctc gaggcgttgt gaggcgcata tggcaggagg atgcagagag
     1381 cctcgggaaa aaactgcacg cagtcaacag gcttaacata ctgggcgtcg ttttcaaggg
     1441 cgcccgggat gccagggagc aagtccttga tgaggtgtgc cggaggttca ttatcatgtg
     1501 acatgtagca tatactctgg cagagggcgg actgacccgc catcgccagg ggggaggaag
     1561 aagtgggaat tcagctcgag cacaggcagc tctcgattac gagggtagct gcgcaaggca
     1621 tggctgagat cagcctacag cgatctgtag cattacttca ggaccatccg gacatatctg
     1681 atgattctgc ccattcgatc ctgtgggccc agggcgtgcc gattgtggcc tcagtggaac
     1741 ccatggacgg gcagatcaaa gtcacaggct acgtgcaatc caccgtgttc tatgtggcca
     1801 aaggcgcccc ggattttgtc gggaaggccg tcatgtccga gccgaggttt gaagcggtga
     1861 taccggcgcc gggcgccacc gtctcggaca gggttaaagt ggatctcacc ctatcgcagt
     1921 tggaggtgga aaccaccggc acacgcgggc tcctcctggc tgccactatc atggcctcag
     1981 caagagcttt gcgggacgag gttgttgagg ccgctgtgtc cgcagaggct acaggcgggt
     2041 cacgcatatc tgtccattcc gaagagataa atgtaaccag catacatgag tcgttttcgg
     2101 agaaggtcga gttttccgag atccttagta tcccagatga aaaagcggct tgcctgggga
     2161 gcgaatcggc atgtggcgcg gcaggaactg ccagagtaat cgggatacag gttggagacg
     2221 ggaaggtaac tcttgacgct gagatttctg tggaaattgc ttcgaccgtt gtcagagggg
     2281 ttccggctct caacatactc cgatttgagc gcgtccccat acatgcgtct ttcgatgttc
     2341 ccgctgctga gaagggaatg actgccctaa cccagctggc gctctcccat gtttccgcga
     2401 tcctcctttc cgatgctgaa gtgagtatcg atggcatcct cgatgtagac gtggacttgg
     2461 tctcatcaga acgtctgtcg gtcatcgttg acatcgactc tgaaacccag gagatagtgg
     2521 atatcgatac ggcggggatt acagtggaga agcttgtggg agagggcatg atcgatctag
     2581 atctgcacga ttcggttgcg cttcaagcgc tcatgaggcg atattcgttc accggaatgg
     2641 aggatgttga aggcagcatc ggattactgg ggctgtccga tatagagacc accgatgatg
     2701 aggtttcgct gaggggactt ctcactgcca gggcaatctt gagcgacgtg gacgaggatg
     2761 aagagggaca attcccgatc aacttcgcgc tcgatatccc catagagttt tcggattctg
     2821 cggaagttcc gggggtcaga gcgggagaca gggcggagcc aaggtccgcg acacacaccg
     2881 tggtagtggg aaaatcggcg ccaaacaggt tggatgtgga gggctcgctc cgtgtagacg
     2941 tggaagttta tcgacctgtc gaactgcaag tggtgacttc ggccgagacc attagtcctg
     3001 tgacccttga tccttacgcg atgactttct acgtggttgg acccggagat acccttgc
//
LOCUS       DAOSAI010000310         3021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_20934_length_3021_cov_3.756912, whole genome shotgun sequence.
ACCESSION   DAOSAI010000310 DAOSAI010000000
VERSION     DAOSAI010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20934_length_3021_cov_3.756912"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..2162)
                     /locus_tag="PLM83_09340"
     CDS             complement(300..2162)
                     /locus_tag="PLM83_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOK71642.1"
                     /translation="MKFITGVNYWPKNKGLAMWRDFDIREIAGDMEAIAKLGMNAVRL
                     FLRWSDFQPEPDRIDRTMMDRFEQVLEAAHKAKIGVIPTFFCGHMSGENWDVPWRGGR
                     DPYTDPEMLRAQALQVRHFAMAYRGDPRIICWDLANEQDVFVKPRDRHSGWLWNRLLA
                     SELKMNDPDHPVTLGTHISSLWEDCGFWPEDLAESLDFLCMHAYPLYSDACPERMDGV
                     RSTYFVPFCAALTRALGRGADVLFEEFGATSQMCAEDVIERYYTTSLYALLAEGVTGA
                     MGWCYGDFSVGDELPYETTPYEVGFGLVDREGCPKGAGRAMAEFCKALDCAGGGVMHK
                     AEVGAAIVVQRRYYENYDPDVTPAQNFRVLFNSYIMARRSGLNPEIVTFDDDLARFEV
                     LFVPSVPRRGAINTSDWRRLTKFVEDGGTLYLSYNGVALEGMGDVFGIDVRYPTNEGE
                     GTLLRLCRPEAVDTMLPIVNRTGRRMVIDPAGAMALWTRDDGSFAVVAAKFGRGTAVF
                     CDFPLELSISYEPNIYQRGAYEEVYKDVVSLSEWQEPAWTDSPDVGATVLQSNTGDLL
                     VLVNFSHELRNVDVWWPGSTTVEVVTRKDELECDSDGRFRFPLPSNEGVILRQR"
     gene            complement(2192..>3021)
                     /locus_tag="PLM83_09345"
     CDS             complement(2192..>3021)
                     /locus_tag="PLM83_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71643.1"
                     /translation="AKLRAVLLVVALCFGFALVSPRAPSVAGAEAYTEPYFEYISSAY
                     SIGISLDPVCIDYISIDPESQGEQRDVLAYVGKPSTLWLGIGGWGTHMPTKPSRVKVL
                     EEEGRTVLAISDIEFVGFLKTTFGEIAPVRADWVFTFYEDFFDMELTWRIQENVDGLQ
                     EAGWALNIDLPRYGDSEEAVRLNQDVNGYPKWIVWYGPDLGVAAAHKEGSTNLRANRW
                     YGNLPSSYVIVHTWWKPGGSGIDWGTYPGGIWRVGFFGGEKQLDAAWALYGELNGID"
BASE COUNT          674 a          942 c          751 g          654 t
ORIGIN      
        1 aactggctgc ttacgaccat cgacgcaata atcatccttc tggaagtctg gttgatcata
       61 gaagcattca acgttctggg cagtacgcaa aaacaagagt taaagcccac ggcatagcaa
      121 cgaattgccc gggccagtgt cccgcttcac ggttaaacca ggtttggggc ttctggcgcg
      181 atgcgggcaa acatctttca catgtagctt cgccgcgccc ccagatagct caaagcgcgg
      241 cgaagctaca tgaagcactt taacccacag acggtacatc aatcccgaca agatggcact
      301 caccgctgcc ggaggataac cccctcattg cttggaagag ggaatctgaa ccgcccatcg
      361 gaatcgcact ccaattcgtc ttttcttgtg actacttcca cggtggttga cccaggccac
      421 catacatcca cgttgcgcag ctcatgggag aagttgacta acaccagcag atcgccggta
      481 ttcgattgga gaactgtagc cccaacatcg ggagagtcgg tccacgcagg ctcctgccac
      541 tccgatagtg agactacatc cttgtatacc tcttcatagg cgccgcgctg gtagatattt
      601 ggctcatagg aaatggacag ctccaacgga aagtcacaga ataccgccgt tccgcgtccg
      661 aacttcgcag caacaactgc gaaagatccg tcatctcgtg tccaaagcgc catagcccct
      721 gccggatcta taaccatccg tcttccggtc ctgttcacga tgggcagcat cgtatccaca
      781 gcttcgggcc ggcatagccg gagtaaggtt ccttcccctt cgtttgtggg atacctgacg
      841 tcgatgccga atacatcccc catgccttcc aaggctacgc cgttatagga caaatacagt
      901 gtccccccgt cctccacgaa tttcgtcaga cgcctccaat ccgacgtgtt tatcgcaccg
      961 cgccgcggaa cacttggaac gaagagaacc tcaaaccttg cgaggtcatc gtcgaatgtc
     1021 acgatctccg ggttgagccc gctccgtctg gccattatgt aggaattaaa gagcaccctg
     1081 aagttttggg ccggggtaac atcggggtca tagttctcgt agtaacgcct ttgcactacg
     1141 attgcggctc caacctcagc cttgtgcatt accccgccac ccgcgcaatc aagcgccttg
     1201 cagaactcgg ccatggcgcg tcctgcgccc ttggggcatc cttctctgtc taccaagccg
     1261 aaccccactt cgtaaggggt cgtctcgtag ggaagctcgt cgccgactga aaaatccccg
     1321 tagcaccacc ccatcgcccc tgtcactccc tcggcaagga gcgcatagag gctggtcgta
     1381 tagtatcgct caatcacgtc ttctgcgcac atctggcttg tcgccccaaa ctcctcgaaa
     1441 agcacgtcag caccgcgccc tagggcacga gtgagggccg cgcaaaacgg gacaaaatag
     1501 gtgcttctta cgccatccat ccgctcaggg cacgcatccg agtagagcgg gtacgcatgc
     1561 atgcagagga agtcaaggga ttctgccagg tcctccggcc aaaaaccgca atcctcccac
     1621 aggctcgata tgtgggttcc aagggtgacc ggatggtcgg ggtcattcat cttcagctct
     1681 gatgcgagaa gccggttcca tagccaaccg gagtgcctat cccggggctt tacgaacaca
     1741 tcctgctcgt tggcgaggtc ccagcagatg atcctgggat cgcccctgta tgccatggcg
     1801 aaatggcgca cttgaagcgc ttgtgccctg agcatctccg gatcggtgta agggtcgcgc
     1861 ccgccgcgcc aaggcacgtc ccagttttct ccgctcatgt ggccgcagaa gaacgttggg
     1921 atcactccta ttttcgcctt gtgggccgcc tcgagcacct gctcgaacct gtccatcata
     1981 gtccggtcaa ttcgatccgg ctcgggctga aaatcgctcc acctcaaaaa gagtctcacc
     2041 gcgttcatgc ccagtttcgc gatggcttcc atgtcgcctg cgatctcacg gatgtcgaag
     2101 tccctccaca tcgcaagccc cttgttttta ggccagtagt tgacgcctgt tatgaacttc
     2161 atatggtgcc ccctccatgc tgcaatctat ctcaatctat gccgttgagc tctccataaa
     2221 gagcccaagc tgcatcgagc tgtttttcgc ctccgaagaa ccccaccctc caaatgccgc
     2281 ctgggtacgt tccccagtcg atgcccgatc cccccggctt ccaccaggta tggactatca
     2341 cataactcga aggcagattt ccataccacc ggttcgctct gaggttcgtt gaaccttcct
     2401 tgtgagctgc tgccacaccg aggtcgggcc cataccacac tatccatttg gggtaaccat
     2461 tgacatcctg attgagccgt acggcctcct cagaatcgcc ataccgtggc aaatcgatgt
     2521 taagtgccca gcccgcctcc tgcaatccat cgacattctc ctgaatgcgc caggtgagtt
     2581 ccatgtcgaa gaaatcctca tagaatgtga atacccaatc cgcccggaca ggagcgatct
     2641 ccccgaatgt cgtcttgaga aagcccacga attcgatgtc tgagatagcc agcacggtcc
     2701 tgccttcttc ttccagaact ttcactcggg acggttttgt cggcatatga gtgccccatc
     2761 ccccgatccc cagccacaga gtggacggct tgcccacata cgcgagaaca tcacgttgtt
     2821 ctccctggct ctcagggtcg atggaaatat agtcgatgca gacgggatcg agcgaaatgc
     2881 ctatggagta tgcggaactg atgtattcga aatatggttc ggtgtaggcc tcggcaccgg
     2941 caacggatgg cgcccggggc gagacgagag cgaacccaaa gcagagtgcg acgacaagca
     3001 atactgctct aagcttggcc a
//
LOCUS       DAOSAI010000311         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_21230_length_2991_cov_3.483311, whole genome shotgun sequence.
ACCESSION   DAOSAI010000311 DAOSAI010000000
VERSION     DAOSAI010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21230_length_2991_cov_3.483311"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLM83_09350"
     CDS             <1..393
                     /locus_tag="PLM83_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71644.1"
                     /translation="VINMLEELQEERDLTYLFIAHDLAMVKHTSHRVAVMYLGKIVEL
                     ADSVELYKNPLHPYTKALMSAIPIPDPDVEAKRERIILEGDVPSPIDPPPGCRFKSRC
                     SYAMKVCGEEEPELRDFGGHHIACHLVK"
     gene            595..1827
                     /locus_tag="PLM83_09355"
     CDS             595..1827
                     /locus_tag="PLM83_09355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOK71645.1"
                     /translation="MPKRISSIYARAISLSTVIILAMAAFACSAQTGVPAQAKVEFYS
                     EAPAPMIEANAAILEDAATGQVLFSMNPDERLAPASITKVMTMLIVMEHAQEGKVSLD
                     DTVVVSREASLMGGSQVYLKEKEEVPIRELLAATAIRSANDASYALAEAIAGSADAFV
                     ELMNARAAELGMSNTHFANPEGLDDPGHYTTARDIAIMSRELLKHPEILELTSTWIGK
                     MRGGTYDLFNTNRLIKVYEGADGLKTGQTDNAGWCLVGTAVRGETRLLSVVLGAASDE
                     KRVGETSKLLDYGFQKFERTRFLDAGAPVGTIRVPSAARQDVTAVAAEDLVLLCPRGF
                     SSETEVHFEPLGQELSAPIKKGQPLGRAVAVMPSGEELGSTLAVAESDVPRANAVVRA
                     WRAVVRFFAELFGGRTRA"
     gene            1860..2849
                     /gene="ispE"
                     /locus_tag="PLM83_09360"
     CDS             1860..2849
                     /gene="ispE"
                     /locus_tag="PLM83_09360"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOK71646.1"
                     /translation="MRVVESKAWAKINLAFDVVGLRPDGYHDIETVMHRVELADEIRV
                     ERLEAGAVSEYEIRVDVFPQVAPAGTENIAYRAAMAFFEAAGKRGAVSIGIGKKIPVA
                     AGLGGGSADAAAVLASMNRLWDVPLAEDELYHVARSVGADVPFCLMCHGDPPRAWAAM
                     ACGIGDDLTPLPPLPGVAVVLAKPSVSLLAGEMYGMWDARRARAMGGRGNARARALAN
                     WMRSQGGSALKGDAVSGSPVAQAVHLIGNELEPVAAERCPEVLQVKAALLELGAMAAV
                     MSGSGPTVFGLFESDDSAHSAARSLRKRAFSFSGRGPLDVIVTHAGPRIPPGS"
BASE COUNT          625 a          710 c          970 g          686 t
ORIGIN      
        1 gttataaaca tgctcgagga gttgcaggaa gaacgcgatc tgacgtatct tttcatcgct
       61 cacgacctgg ccatggttaa gcatacgagc caccgtgtgg cagtgatgta cctcgggaag
      121 atcgtggaac ttgcagacag tgtcgagctt tacaaaaacc cgcttcatcc ttatactaag
      181 gcattgatgt cggcgattcc cattcccgac ccggatgtgg aggctaagcg cgaaaggatc
      241 attcttgaag gggatgtccc gagcccgatc gatcctcctc caggatgcag gttcaaatcg
      301 agatgcagct atgccatgaa agtctgcggg gaagaagaac ccgagctgcg cgatttcggc
      361 gggcatcata tcgcctgcca tcttgtaaag tagactccca tcgtagggac gcagaccctt
      421 gacgcacttg ttcgtgcggg gggtgctgag cagtacaggt ttttggggcg catgggctca
      481 gcggcacaaa ctttaatgcc gctgagcgat gcgcccgttc tcataacctc atcgcctgcg
      541 ggcagaatct gtagtatgag atatgaagat cgtttgcttc gaggaggctc taaattgcca
      601 aagagaataa gttctatcta tgctcgcgcc atatccctgt cgactgtcat cattcttgca
      661 atggcagctt tcgcctgttc tgcacagaca ggggttcctg cccaagccaa agtcgaattc
      721 tacagcgaag ctcctgcgcc gatgattgag gcgaatgccg ccattcttga ggacgccgcg
      781 accggccagg tcctgttctc catgaatcct gatgaaaggc tggcgcctgc ttcgataacg
      841 aaagtcatga cgatgttgat cgtcatggag catgcccagg aaggcaaggt ttcactggat
      901 gacactgttg tcgtgagccg tgaggcatct ctcatggggg gctcccaggt ttacttgaag
      961 gagaaggaag aagtccctat cagggagctg ttggcggcta cagccataag gtcggctaat
     1021 gatgcaagtt acgcgctcgc tgaagccatt gcggggagcg cagacgcttt cgtcgaactg
     1081 atgaatgctc gggccgctga acttggcatg tcaaacaccc attttgccaa cccggaaggc
     1141 ctcgacgacc ctggccacta cactacggcc agagatattg cgataatgtc ccgcgagctt
     1201 ctcaagcacc ccgagattct ggagttgacc agcacttgga tagggaagat gcgcgggggg
     1261 acctacgatc tcttcaatac taataggctt atcaaggtat acgagggtgc ggatggcctg
     1321 aagaccgggc agacggacaa cgccggctgg tgtctggtgg ggaccgctgt gcgcggggag
     1381 acgagacttc tttcggtagt tcttggcgct gcgagcgatg agaagagggt cggagaaacc
     1441 tcgaagctct tggattacgg tttccagaag tttgagcgaa cgcgcttctt agatgctggc
     1501 gcgcctgtag ggacgattcg tgttccctct gccgcaaggc aggatgtgac ggcagtggcg
     1561 gcagaggacc ttgtgttgct ttgcccgagg ggattttcat ctgagacaga ggttcatttt
     1621 gaacctcttg ggcaggagtt atctgctccg ataaagaaag gacaacctct gggtcgcgca
     1681 gttgcggtaa tgccgtctgg agaggaatta gggtcgacgc tcgctgtggc tgagtctgac
     1741 gtcccacggg ccaatgcggt agtgagggcg tggagggcgg tagttcgctt ctttgcggaa
     1801 ctgtttggcg gtagaactcg agcatagtag tttgtgcatt acctgatgga tgtggtctca
     1861 tgcgtgtggt tgaaagcaaa gcctgggcaa aaatcaatct tgctttcgat gttgtcgggc
     1921 tcagaccgga cggttaccat gatatcgaaa cggtgatgca ccgtgtagag cttgcagacg
     1981 agattagggt tgagcggctt gaagctggtg cagtgtcgga atatgagata agagttgatg
     2041 tcttcccgca ggtggcgccg gctggtacgg agaatattgc gtatcgcgcg gctatggctt
     2101 tttttgaagc cgccggaaaa cgaggcgcgg tatcgatagg gatcggaaag aagatacctg
     2161 ttgccgccgg gctcggggga ggaagcgcag acgcagccgc ggttttggct agcatgaatc
     2221 ggctgtggga tgtgccactc gcagaggatg aactgtacca tgttgcccgt tcggtcggcg
     2281 cggatgtgcc gttttgtctt atgtgccatg gcgatccgcc tcgggcttgg gcggctatgg
     2341 catgtggcat tggggatgat ctgacgccct tgcctccgct gccaggagtt gccgtggtcc
     2401 ttgcgaaacc ctcagtctct ctcctggcgg gcgaaatgta cgggatgtgg gatgctcgac
     2461 gagcgcgggc gatgggtggg cgcggcaatg cgagggctcg ggctctggcg aactggatgc
     2521 gatcgcaagg cgggagcgct ttgaaagggg atgcggtatc tggttcgcct gtggcgcagg
     2581 cggtgcatct gattgggaat gagctggagc cggttgctgc cgagagatgc ccggaggtgc
     2641 tccaagttaa ggcggcgctg ctggagttgg gggctatggc tgcggttatg agcggcagtg
     2701 gacctactgt attcgggttg tttgagtcgg atgattctgc acattctgcc gcacggagcc
     2761 tcaggaaacg ggcgttctct ttttccggaa ggggcccgct cgatgtcatc gttacacatg
     2821 ccgggcctag gattcctcct gggtcttagg agcatatcga aggatttgcg aggcacacaa
     2881 ttcaatccca aatagggaat gaggtgagag gcgcaatgcg agtggatgtg gcaattctgg
     2941 ccggcgcgga taacgatggg cgtctggccg aggtttcaga cgcgaaatac g
//
LOCUS       DAOSAI010000312         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_21573_length_2959_cov_4.985882, whole genome shotgun sequence.
ACCESSION   DAOSAI010000312 DAOSAI010000000
VERSION     DAOSAI010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21573_length_2959_cov_4.985882"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1507
                     /locus_tag="PLM83_09365"
     CDS             125..1507
                     /locus_tag="PLM83_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008866262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOK71647.1"
                     /translation="MRPRDEYAEPYRVKMVEPIKMTTREERIGKIEGAGYNVFNLRAE
                     DVYIDLLTDSGTPAMSDTQWAALMCGDEAYAGSKSFYRFRDVVRNITGYEYVIPAHQG
                     RGAEHVIMTALVKDGDAVLGNMHFDTTMGHILLRGAEPVNLLKKEGYEVESTEPFKGN
                     IDIEALERELDKRGSEKVPFVLMTVTCNSNGGQPVSMENIKAASEIAHKHNVPLFFDC
                     ARFAENSWFIKNREPGYENKSLLEIAQEMFSYGDGCIMSAKKDALVNIGGFAAVRDPD
                     IYHKAIEWEIPFEGYLTYGGLAGRDLEAMAQGLSEVLDDDYLEDRIGQVAYLADLLLE
                     RGVPVITPAGGHGVYVDAKAVLPHIPQSEFPAQAMTVELYIEGGVRGVELGACAFART
                     DPATGETIYPELELVRLAIPRRVYTDRHMLQVARAFEGILERRSSIRGLRITYEAPVL
                     RHFTAEFERV"
     gene            1623..2003
                     /locus_tag="PLM83_09370"
     CDS             1623..2003
                     /locus_tag="PLM83_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK71648.1"
                     /translation="MKRALLGAAIVLMCTCLTGCTLYFGGWATASGRVVECAEGSEGQ
                     VIQIPVCGAIVTLTSASNPDYSLNCDTDENGYWFTPVKVKCDMYKVTAKMDGYADAGP
                     IDFPIAKRGAHCIMERPIVMQKHK"
     gene            2138..>2959
                     /locus_tag="PLM83_09375"
     CDS             2138..>2959
                     /locus_tag="PLM83_09375"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOK71649.1"
                     /translation="MKHLIHTRRRNKVSVIGAGLVGSTYAYTLMLSGAASEIVLLDAC
                     VQRAEGEAMDLSHGAPFAPPVRIRSGDWEATSNSDIVVITAGAAQKPGETRLDLAKRN
                     VEVFRDIVPKAAAASPDSIMLVVTNPVDLMAYAAIVMSGFPASRVIGSGTLLDSARLR
                     QLIGEHCKVAPGNVHAYIIGEHGDSEVPLWSTANIAGLRLDEYCPVCGRACDSSIRTR
                     IFEEVRDAAYAVIERKGATYYAIALALTAVTRAILRDESAVMTVSCLIDGFCGARD"
BASE COUNT          688 a          732 c          895 g          644 t
ORIGIN      
        1 gtgtgacagg caatggggca gcctagactt ggcgtaatct ccgggcttct ccaagaggga
       61 aacgggtttt tgaaggatgg gagtatacaa tggcgaatat ctcctaactg tgaggtgatt
      121 tgccatgcga ccgcgtgacg agtatgcaga accgtatcga gtcaagatgg ttgaacctat
      181 taagatgacc acgagggagg aaagaatcgg gaaaatcgag ggtgctgggt acaacgtctt
      241 caacctcaga gccgaagatg tatacatcga ccttctcacc gacagtggaa cgccagcaat
      301 gagcgacact caatgggctg ccctcatgtg tggggatgaa gcctacgctg gcagcaagag
      361 tttctaccgc ttccgggacg tagtgcggaa catcacgggc tacgaatacg tgatcccggc
      421 tcaccagggg cggggcgctg aacacgtgat catgacggca cttgtgaaag atggggatgc
      481 ggtcctcgga aatatgcact ttgacaccac catgggtcac attctcctcc gaggcgcaga
      541 accggtcaat ctcttgaaga aggaggggta tgaggtcgaa agcaccgaac cgttcaaagg
      601 caacattgat attgaggccc tcgagaggga gcttgataag cgcggatccg agaaggttcc
      661 gttcgtgctc atgacggtaa cgtgcaatag caatggcggg caacccgtat ccatggagaa
      721 cataaaggcg gccagtgaga tagcgcacaa acacaatgtg ccgctatttt ttgattgtgc
      781 caggtttgcc gaaaactcgt ggtttatcaa gaatcgcgaa cccggatatg agaataagag
      841 cttgctcgag atcgcccagg agatgttctc ctacggagac ggctgtataa tgagcgcaaa
      901 gaaagatgcg ctggtcaata tcggagggtt tgccgccgtg cgtgaccccg acatctatca
      961 taaggccatc gaatgggaaa tacccttcga aggctatttg acatacggcg gccttgccgg
     1021 acgagacctc gaggccatgg cgcagggtct ttcggaagtg ttggacgacg actaccttga
     1081 ggaccgcatc ggtcaagtcg cctacctcgc cgacctttta ctggagaggg gagtgccggt
     1141 gataacgccc gcaggcggac atggcgtata tgttgatgcc aaagccgtgt tgccccatat
     1201 tccgcaatct gagtttccgg cccaggcgat gacggtggaa ctgtacatcg agggcggggt
     1261 tcgtggcgtg gagctcggcg catgcgcctt cgcccggact gatccggcca ctggcgagac
     1321 tatctatcca gagctggaac tggtcaggct ggctattccg cgacgagtct acactgacag
     1381 gcacatgctc caagtggcaa gggccttcga gggcatcctt gagaggcgga gctccatccg
     1441 gggactgcga attacgtatg aggctccggt gctgcgccac ttcactgcgg aattcgaaag
     1501 agtgtagatg ggcttggtct gaaaaagccg ggttgtctta gctcgcttgg gtccaagatc
     1561 tgcaggataa ttgttgctta tgacgaaatc ttgcgcatag gccctaaatg gaggaatggc
     1621 agatgaagcg tgctttgctc ggtgcagcta ttgttctgat gtgcacatgc ttgaccggat
     1681 gcactctgta cttcggaggg tgggcaactg ccagtggtcg tgtcgttgag tgcgcagagg
     1741 gcagcgaggg gcaggtcatt cagattcctg tttgtggcgc gatagtaaca ctcacatcag
     1801 cttccaaccc cgattacagc ttgaactgtg atactgacga aaatggatat tggtttactc
     1861 cggtcaaagt taagtgcgat atgtacaaag tgacggcgaa gatggatgga tacgctgatg
     1921 cggggccgat tgactttcca atagcaaagc gaggcgccca ctgcattatg gagaggccca
     1981 ttgtaatgca gaagcacaag tagccgccga ttaactgagt tgacaggctg cataagttgt
     2041 tgacaagacg ccctttccac ttcaggcggg gagggcgttt gcttactcgc ttctctgggg
     2101 agcatattca tgagcacatc gaaggagcga gttcgcaatg aaacacctta tccacactag
     2161 gcggcggaat aaagtctctg tgatcggcgc tggccttgta gggtcgactt atgcatatac
     2221 tttgatgctt agcggggcag cgtcagagat agtcctgctg gacgcctgcg tccagcgggc
     2281 ggagggcgaa gccatggacc tttcgcatgg cgctcctttt gcgcctcctg tccgcataag
     2341 gagcggagac tgggaggcca cctccaattc ggacattgtg gtgataactg ccggggcggc
     2401 gcagaaaccg ggagagacca ggctcgacct cgccaaacgc aacgtggagg ttttccggga
     2461 catagtgccc aaggctgctg ccgccagtcc ggattcgata atgctcgtcg tcaccaaccc
     2521 agtcgatctc atggcgtatg ccgcgatcgt gatgtcgggg tttcctgcta gtcgagtcat
     2581 aggttctggc acccttcttg acagcgccag gttgaggcaa ctcatcggcg agcattgcaa
     2641 ggtcgcgcct ggaaatgttc atgcatacat catcggcgag cacggagact cagaagtgcc
     2701 tctttggagc accgcaaaca ttgcaggctt gcgtctggac gagtattgcc ctgtgtgcgg
     2761 gagggcatgc gacagctcca tcagaacccg aatctttgag gaagtgcgcg atgcagcata
     2821 tgcagttata gagcgaaaag gcgccacgta ctatgcaata gccttggctc tcaccgcagt
     2881 cactcgcgcc atactccgcg atgagagtgc tgttatgact gtctcttgcc ttatagatgg
     2941 attctgcggc gcccgcgac
//
LOCUS       DAOSAI010000313         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_21602_length_2956_cov_3.613582, whole genome shotgun sequence.
ACCESSION   DAOSAI010000313 DAOSAI010000000
VERSION     DAOSAI010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21602_length_2956_cov_3.613582"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="PLM83_09380"
     CDS             <1..1463
                     /locus_tag="PLM83_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOK71650.1"
                     /translation="LTYTNVLAMLELAGVPLEADARHEGDPIVIAGGPCASNPEPMAA
                     FFDLLVTGDGEEAIVDLMSEVARAKREGAKRADIVNRLAVIDGVYCPRLKNPVSRRVV
                     TDLDEAEFPRKFVVPFLEAVHDRVALEVMRGCTRGCRFCQAGMIYRPVRERGLDSLCS
                     LAKDLVKSTGYDEISLLSLSSADYTQVEELSRKLVQEYADKGVAVSLPSLRVDSFSLE
                     LAKELEKSRRTGLTFAPEAGTQRMRDVINKGVTEEDLMSAAADAFESGWDLIKLYFMI
                     GLPYEEDEDLDGIADLSRRVLELGRNMAGKRNKAGRVRVNVSVSSFVPKPHTPFQWHA
                     QNSREELMRKQNYLKERMRVRGLTVSFHNVEASFLEAAFARGGRELAPAIARAAALGC
                     RFDGWSDQFRPDLWAQAFKDVGIDSSAWANKSYAYDEPLPWDHIDMGVSKRFLVREAE
                     RAARAELTPDCRTGACAACGACADGVKTRLAGEAWR"
     gene            1471..2190
                     /locus_tag="PLM83_09385"
     CDS             1471..2190
                     /locus_tag="PLM83_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOK71651.1"
                     /translation="MVLRVRITKGEEARFISHLGFMRAFERAIRRAEIPVAYSEGYHP
                     LPKFSFATALGVGISSEAEYVDIQIDPDGPKISVEEAFDALDRNMPPGIAVVAACWYL
                     GKPALASLIEYASYRASGCGALHEAAEEFMAMDASLGTITTKSGKKEEDIRKLVYSIS
                     MTDDESLEFICACGGRAHLRPDNLLVHLSSIAGIECDVKRSRIHRTGLFVVEDGELVS
                     PMDYKCKDLVIAKGWMPRELS"
     gene            2191..>2956
                     /locus_tag="PLM83_09390"
     CDS             2191..>2956
                     /locus_tag="PLM83_09390"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOK71652.1"
                     /translation="MSREIVVNSDNGETRAAILEDGKLVDLFVERSVHPRYAGNVYKG
                     VVENVLPGMQAAFVNIGLERNAFLYVDDALAGHNGRNSRVVREADGEEISVPRKKSRS
                     IKDILKPGQEIMVQVTKEPIGAKGARVVTDVTLPGRYVVLMPTVDYVGVSRRIEEEGE
                     RDRLRKIAQASKPRRMGVIVRTVAEGKSQEEIASDIQFLVKLWRRIQGRNRRAQGATL
                     LHQEYDLAFRLVRDHFAADVTRFVVDDAKEHKKVLDL"
BASE COUNT          675 a          646 c          947 g          688 t
ORIGIN      
        1 aactgacata caccaatgtg ctggcaatgc tcgagttggc tggtgtgccg cttgaggctg
       61 atgcccggca cgaaggcgac ccgatcgtga ttgcaggtgg gccatgtgcg tccaatcccg
      121 aacccatggc ggcatttttt gatctgcttg tgaccggcga tggggaagag gcgattgtgg
      181 acttgatgtc cgaggttgcc agggcaaaaa gagagggggc gaaacgcgcc gacatagtaa
      241 ataggcttgc ggtgatagat ggtgtctatt gcccgcgcct gaagaaccct gtaagccgca
      301 gggtggtgac ggatcttgac gaggccgagt ttccaagaaa gtttgtggtg ccttttctcg
      361 aagctgtaca cgaccgggtt gctctggaag tgatgagggg ttgcacaaga gggtgcagat
      421 tttgccaagc cggcatgata taccggccag tgcgggaacg cggactcgac agtctctgct
      481 cgctggcgaa agatcttgtc aaaagcacgg gctatgacga gatatctttg ctttcgcttt
      541 caagcgcgga ttacactcag gttgaggagt tgagcagaaa gctcgttcag gaatacgcag
      601 acaagggagt agcggtttcc ctgccttctt tgcgtgtgga ttcgttttct ttggagcttg
      661 ccaaggagct agagaagtcg cgaaggacag gcctgacttt tgcgcctgag gccggaaccc
      721 aaaggatgcg ggatgtgatc aacaagggcg ttaccgaaga ggatctgatg tctgccgctg
      781 cagacgcctt tgaatcggga tgggatctca tcaagcttta tttcatgata gggctgccgt
      841 atgaggagga cgaggatctg gatggcatag cagatctctc tcgtcgtgtt ttggagcttg
      901 gacggaatat ggcggggaag cgaaacaagg cgggcagggt ccgagtaaac gtgagcgtat
      961 cgtcgtttgt tcctaaacca catacccctt ttcagtggca cgcgcagaat tcccgggagg
     1021 aattgatgag aaagcagaat tacctgaagg aacgaatgcg cgtgcgcggc ttgacagtct
     1081 cgtttcacaa tgtggaggca agcttcctgg aagcggcgtt tgcgagaggg ggcagagagc
     1141 ttgctccggc gatagcgcgc gcggcggcgc tcggatgcag gtttgacgga tggtcagacc
     1201 agttccgccc tgacctatgg gcgcaagctt ttaaagatgt tggcattgac tcttccgcat
     1261 gggccaataa atcctatgca tatgatgagc cgctgccatg ggatcacatc gacatgggag
     1321 tctcgaaaag atttctcgtg cgggaagcag aacgtgccgc cagggctgaa cttacccccg
     1381 attgccgcac tggtgcttgc gcagcctgtg gtgcctgcgc ggatggcgtc aagacgcgtc
     1441 ttgccggcga ggcatggagg tgaacggggt atggtgctga gggttcgcat tacaaaaggc
     1501 gaagaggcgc ggttcatctc acaccttggt ttcatgagag cttttgagcg cgccataagg
     1561 cgtgctgaga ttccggttgc atattctgaa ggctaccacc ctttgcccaa gttctcattt
     1621 gccaccgctc ttggggtcgg aatttccagc gaagcggaat atgttgacat tcagatagat
     1681 cctgacggtc ccaagatcag cgttgaggag gccttcgacg cgctggacag gaacatgcct
     1741 ccggggattg cggtcgtggc ggcctgctgg tatctgggta aacctgcact cgcatcactt
     1801 attgaatacg cttcctatag ggcgagtgga tgtggcgcct tgcatgaagc cgcagaagag
     1861 tttatggcca tggatgcctc cctagggact atcaccacga agagcgggaa aaaagaggag
     1921 gatatcagga agctcgttta ttcaatatcg atgacagatg atgagagttt ggagtttatt
     1981 tgtgcgtgtg gcgggcgcgc tcatctcagg cctgataacc ttcttgtgca cctaagttcg
     2041 atcgccggga ttgaatgtga tgtcaagcgg tcccggatcc atcggactgg tttgtttgtt
     2101 gttgaggatg gggaactcgt gtcccctatg gattataaat gcaaagattt ggtcatcgct
     2161 aagggttgga tgcccaggga gttgagttga atgtccagag aaattgttgt caactccgac
     2221 aatggcgaaa caagggccgc catcctggag gatggaaagc ttgtggatct ttttgtggag
     2281 agatcagttc atccccggta tgcggggaac gtctacaaag gagtagtcga gaatgttctt
     2341 ccggggatgc aggcggcttt cgtcaatata ggtttggagc gcaacgcgtt tttgtatgta
     2401 gatgatgcct tagcagggca taatggccgc aattcccgcg tagtacggga ggcggatgga
     2461 gaagagattt cggttccgag gaagaagtcg cgatcgatta aggacatctt aaagcctggt
     2521 caggagatca tggttcaggt tacgaaagaa cccataggcg ccaagggcgc aagggtagtg
     2581 acggatgtca ctcttccagg aaggtatgtt gtgctcatgc ccacggtgga ctatgttggt
     2641 gtgtccagac gcatagaaga agagggcgag cgtgacagac tgcggaaaat cgctcaggcc
     2701 tcgaaaccgc gtcgaatggg cgtgattgtg aggacggttg ccgaagggaa gagccaggag
     2761 gagatcgcct ccgatattca gtttcttgtg aaattatgga ggcgaataca gggcaggaac
     2821 aggagggcgc agggcgccac gttgcttcac caagagtacg acttggcttt caggttggtt
     2881 cgcgatcatt ttgctgcgga tgtaaccagg tttgtggtgg atgatgctaa agagcataag
     2941 aaagtcctcg atctgt
//
LOCUS       DAOSAI010000314         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_21910_length_2925_cov_4.509408, whole genome shotgun sequence.
ACCESSION   DAOSAI010000314 DAOSAI010000000
VERSION     DAOSAI010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21910_length_2925_cov_4.509408"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLM83_09395"
     CDS             complement(<1..244)
                     /locus_tag="PLM83_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK71653.1"
                     /translation="MTAPVSQYTAEDIQVLEGLEAVRLRPSMYIGDTGPRGLHHLIYE
                     VVDNAIDEVLADFCDRVIVVLHSDGSISVEDNGRGIP"
     gene            complement(494..2155)
                     /gene="pgm"
                     /locus_tag="PLM83_09400"
     CDS             complement(494..2155)
                     /gene="pgm"
                     /locus_tag="PLM83_09400"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HOK71654.1"
                     /translation="MKIHELAGRSAPLELLINAPRLVASFYALKPDASDPLQRVAFGT
                     SGHRGSSLKKSFNEDHILAICQAICEYRASRGIAGPIFVGMDTHALSEPALMTAVSVF
                     AANGADVVIQEGFGYTPTPVISHAILTYNLKKNSGLADGVVITPSHNPPEDGGFKYNP
                     PQGGPADPETTKVIQDRANALLENGLKGVKFISFEKAMASGVVHRSDYIKPYVDELNS
                     VVDMEAIAASGIKIGVDPMGGAAVDYWGVIAERYGLNLHAVDRRVDPTFSFMTVDHDG
                     KIRMDCSSPYAMARLIGLREDFDIAFGNDPDVDRHGIVTKSGLMNSNHYLSVAVWYLF
                     QHRPAWEEGISVGKTIVSSSMIDRVARGLGRKVVEVPVGFKWFVDGLLSGSLGFAGEE
                     SAGASFLRRDGTVWTTDKDGIIMGLLAAEITAITGKDPAEHYKELERRFGRTCFARMD
                     VPATPEQKAALGRLSPDDVQTDTLAGECITAILTHAPGNSAPIGGIKVVTEGGWFAAR
                     PSGTEDVYKIYAESLRGEGHLAKILEEAQNIINAAFATARIQGQH"
     gene            2237..2845
                     /locus_tag="PLM83_09405"
     CDS             2237..2845
                     /locus_tag="PLM83_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOK71655.1"
                     /translation="MQTLIEWLVSTIGRLGYPGIAALMFLESSFFPFPSEVVMPPAGY
                     LAAQGRMNLWVAITMGTLGSLLGALFNYWIAVRFGRPFLERYGKYLFISEETLKKAEQ
                     FFLNHGHISTFIGRLLPGIRQYISLPAGLARMKIPTFCIFTALGAGIWSAILAWVGYW
                     VGYNQELVAQYLHSITITIVAACAIIIALYLHHLRKKKDHAK"
BASE COUNT          712 a          841 c          690 g          682 t
ORIGIN      
        1 ccggtatgcc gcgaccatta tcttccacag atatactgcc atcagaatga agcaccacaa
       61 taacccggtc gcagaagtcg gccaatacct catctatggc gttatcgaca acctcataga
      121 tcaagtgatg aaggcccctg gggcccgtgt cgccgatata catgctaggc ctcaggcgca
      181 ctgcctctaa tccttccaat acttgaatgt cctctgcggt gtattgtgag accggtgcag
      241 tcatgctttc tttcctcctt gcaaaacctc gtgccttcac gaaaaaactt actgccgctt
      301 gaccaacatc agcggcgcaa tctccgacat caaaacactc ccctccgcag gcagatcaac
      361 gcctcatatt catagcaaac aagggtttta cttcgagcga caaaaacaca gaaattgctc
      421 ctgcggaagg cgggcacatc tatatgcgca tttgtattat accacagaac ttcacatctt
      481 tagacgcagg ctatcaatgc tggccttgaa tcctggccgt ggcgaaggcg gcatttatga
      541 tattttgggc ctcttccaat atcttggcca aatgcccttc acctctaagg ctttccgcat
      601 atatcttata aacatcctct gtgccagaag gccttgcggc gaaccaaccc ccttcggtga
      661 ccacctttat tccgcctata ggggcactgt tgccaggagc atgagtgagg atggcggtga
      721 tgcattcgcc ggccagcgta tcggtttgca cgtcatcggg agagaggcgc cctaaggcag
      781 ccttctgttc cggcgtagct gggacgtcca tcctggcaaa gcaagtcctg ccgaaacgcc
      841 tttccagttc tttgtaatgt tctgctgggt ctttccccgt aatggccgtg atctcagcag
      901 ccagaagccc cataatgatc ccgtctttat ctgtggtcca aactgtgcca tccctgcgca
      961 agaaagaagc tccggcgctt tcctcccccg caaatcccag cgaacccgac agcagcccat
     1021 cgacaaacca cttaaacccg accggtacct ccaccacttt cctgcccaga cctcgagcga
     1081 cgcggtcgat catggagctc gagacaatcg ttttgcccac agatatgccc tcctcccagg
     1141 ccgggcgatg ctgaaagaga taccagactg ctacggatag ataatgattt gagttcatga
     1201 ggccactttt agtaactatg ccgtggcgat cgacatccgg atcgttaccg aaggcaatgt
     1261 caaagtcctc cctcaaaccg atcaggcgag ccatagcgta tggcgacgaa cagtccatac
     1321 gaatcttgcc atcgtgatct accgtcataa aagaaaatgt cgggtcgact ctccgatcca
     1381 ccgcgtgcag gtttaaaccg tatctttcgg ctatgacacc ccaataatct acggccgctc
     1441 cacccatggg atccacgcct atctttatgc ccgatgcggc tatggcctcc atgtcgacca
     1501 cgctgttcaa ttcatcgaca tatggcttaa tgtagtcact tcggtggact actccagagg
     1561 ccatggcctt ctcaaaagag atgaacttca cacccttcaa cccattctcc agcaaggcgt
     1621 tggctcgatc ctgaatcacc ttcgtcgttt ctggatcagc tggcccacct tgaggagggt
     1681 tatatttgaa gccgccgtct tcgggaggat tgtgagacgg ggttatgacg acgccgtcgg
     1741 ccaaaccgga attcttcttg aggttatagg tcaatatggc gtgagatatg acaggtgtcg
     1801 gcgtatagcc gaaaccctcc tgaatgacca catccgcccc gtttgcggca aacacagaca
     1861 cagccgtcat cagagcgggc tcagagagcg cgtgcgtatc catacccaca aaaatcggcc
     1921 ctgcgatgcc acgagaagcc ctgtattcgc atatggcctg acatatggct aatatatgat
     1981 cctcgttaaa gctcttcttc aaagaagaac cgcgatgtcc cgacgtgcca aaggcgaccc
     2041 tctgaagcgg atcgctcgca tcgggtttca aggcataaaa acttgctacc aagcgcggcg
     2101 cgtttatcaa cagttcgagc ggagccgaac gaccggcaag ttcatgaatt ttcactctta
     2161 acccctcccg tttcaacctt tgttctatta tactgaatcg tgcgctttca aaccgatgga
     2221 gcggagtgat caaaaattgc aaaccctgat agagtggctc gtatcgacga tagggaggct
     2281 ggggtatccc ggcatcgcag ccctcatgtt tttggagtcc tcgttcttcc catttccgag
     2341 cgaggtagtg atgcctcccg cagggtattt ggcagcccag ggaaggatga acctctgggt
     2401 tgcgattaca atgggaacgc tcggaagcct cttaggggcc cttttcaact attggatagc
     2461 ggtgcgcttc ggaagacctt tcctcgaacg gtacggcaaa tatctcttca tcagcgaaga
     2521 gacattgaaa aaggcagagc aattcttcct caatcacggt catataagca cgtttatcgg
     2581 caggctcctc cccgggatca gacaatacat atcgcttccc gccggcctgg cgaggatgaa
     2641 aataccgacc ttttgcatct tcaccgccct cggcgctggc atatggagcg cgatattggc
     2701 ttgggtggga tattgggtcg ggtacaatca agaactggtc gcgcaatacc tacactccat
     2761 aaccattacc atcgttgccg catgcgccat cataatagcg ctttacctac accacctacg
     2821 taagaagaaa gatcacgcta aatgaggaga ccttgcgaga tgcccttcga acctgcctat
     2881 tttaagctcg gaattgaaga gatagagaat cgagcgcgca aagcc
//
LOCUS       DAOSAI010000315         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_21984_length_2918_cov_4.709745, whole genome shotgun sequence.
ACCESSION   DAOSAI010000315 DAOSAI010000000
VERSION     DAOSAI010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21984_length_2918_cov_4.709745"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1187
                     /locus_tag="PLM83_09410"
     CDS             246..1187
                     /locus_tag="PLM83_09410"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOK71656.1"
                     /translation="MNPNIEMLAKSAIEEMPEGIVVLDCDGRVVISNRTARSEWGVIV
                     GEDFAACAESNCSCADGGRESGYACKRLKHVVRRIKLTDGEGSRLGDVVCGFSDYESR
                     SDSSDARGYAIRNVANAMIAVISEGVEGLVKSIGVIDHQTARHSFRVRNMSVAMARMR
                     WGTGGDYRDLALAGTLHDIGKALIGEEVLAKAGSLSAEEWAEVRKHPVIGEKMLLQPA
                     AFMAQIAKAVRHHHERYDGAGYPDGLSGDAIPEMARILAIADSFDAMTSDRPYRLALL
                     RSEAIKEIAENAGTQFDPDWVEVFMELMKSERSPFMN"
     gene            1318..1521
                     /locus_tag="PLM83_09415"
     CDS             1318..1521
                     /locus_tag="PLM83_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HOK71657.1"
                     /translation="MANRRPIVSDEVKYEIARELGFADKIQVGADGYDYGNITTREAG
                     LIVRSLIEKAQRAMADQLTRRGQ"
     assembly_gap    1568..1667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2089..>2918
                     /gene="galE"
                     /locus_tag="PLM83_09420"
     CDS             2089..>2918
                     /gene="galE"
                     /locus_tag="PLM83_09420"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOK71658.1"
                     /translation="MLAVGGAGYVGSHVVRSLAQAGHSVIVLDNLSTGHPEAVDVARR
                     VAGGGCVELVVGDVGDGTRVRELLVSKEIDAVMHFAAASLVGESMRNPSKYWRNNVGC
                     AVELLDAMAEAKVDKIVFSSSAAVYGEPTTTPIREDHPKAPTSTYGMTKLAIEHMLIG
                     YDQAYGIRSISLRYFNAAGAHGSGEMGEAHDPETHLIPIILSAALGIRENISVFGTDY
                     PTPDGTCIRDYIHVDDLAEAHLSAMDALSRGAATTAYNLGNGSGFSVLEVLREAERAV
                     G"
BASE COUNT          642 a          664 c          849 g          663 t
ORIGIN      
        1 atgtggagta taagcttgga aaccacgccc tatattgaca ccattggttt ggagttctat
       61 acttctttgt tggtatttga atttgtcatg ccgatattcc gttccaggtt ggtcggagcg
      121 cagatttatg gtgtgctccc tgagttggtg ttggcgggcg actgaatttc cgcaaatgaa
      181 gcgccattgc acactcctgg tcggtttgat atagagcacc atttctttca ggaggttgcc
      241 cgcagttgaa tccgaacata gaaatgttgg caaaaagcgc gatcgaggag atgccggagg
      301 gtattgtagt gcttgactgc gacgggaggg ttgtgatttc gaaccgaacg gccagaagtg
      361 agtggggggt catagtcggg gaggatttcg cagcttgcgc cgaatccaat tgttcttgtg
      421 cagatggcgg cagagaatct ggctatgctt gcaagaggct caagcacgtg gtcagacgga
      481 tcaagctgac cgatggcgaa ggttccaggt tgggcgatgt tgtatgcggc ttttcagact
      541 atgagagtag aagcgattcc agtgatgcca gaggctacgc gatcaggaat gttgctaacg
      601 ccatgattgc tgtcatttca gaaggcgtgg aagggctggt aaagtccata ggcgtgatcg
      661 atcaccagac cgcacgacac tcttttcgag tgcgaaacat gtctgtggca atggccagaa
      721 tgaggtgggg aacgggcgga gactaccgag atttggcttt ggctggtaca ctgcacgaca
      781 taggaaaagc gttgattggg gaagaggtat tggcgaaggc cggatcgctt tctgctgagg
      841 aatgggctga ggtgcggaag caccccgtta tcggagagaa aatgcttctg caacctgccg
      901 cttttatggc gcaaattgca aaagccgtaa ggcatcacca tgaaaggtat gatggagcag
      961 gctacccaga cgggctttct ggggacgcta tacccgaaat ggccaggata cttgctatag
     1021 cagacagctt tgacgctatg acatcggacc gcccctatcg cctagctctg cttcgttccg
     1081 aggctatcaa agaaattgcc gaaaatgcgg ggactcagtt cgatcctgac tgggttgaag
     1141 ttttcatgga acttatgaaa agtgaaagaa gcccatttat gaactgatat ggcgtttgac
     1201 ggttgtctgc aggaattcag gctgcttgga ggttgcggcg aatgccgcca ctttacccag
     1261 cagcatgcct gtttttctgg gacgtttgcg tggaaccata ctcttggggt gattgccttg
     1321 gctaatcgtc gtcccatagt ttcagacgaa gtcaaatacg aaatcgctcg cgaacttggc
     1381 ttcgccgaca aaatccaagt gggcgccgac gggtatgact atggtaacat cactacccgc
     1441 gaagctggtt tgatcgtccg aagccttatc gagaaagcgc agcgggcgat ggctgatcag
     1501 ctgacgaggc gggggcaata gcccccgccc caaacatttg ctgatgaaca gtgtctgtgc
     1561 cacgtgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca tggccaaagc
     1681 gtaaagcagc gaggctacag gattattgca gggtcatctt gccaaagttc tccaggaact
     1741 gattgttcag gcgtatcccc aaaagccttt gaggcccgga caagaagcca aggtctaagc
     1801 ggaggttgga aacgaaccta gccaacttcc agtcacatcg cgatcgcaag tgctcaaggt
     1861 ttggacagag gctgactaaa agttggtcgc tcttccccta aaacccgctt gcgccgacaa
     1921 ttcaatctac ggtgcaaagc aacctcgaat ccggctttgg ttccctgggc ggtgcccgcg
     1981 cgagggtcgt cagcgccgcc atgaattgcc tggcagtgag aaggtgagaa tgatcctcct
     2041 gtcgaagtgc actctgtgtt tcttttgaag ggggattttg ctttgagagt gcttgcagtt
     2101 ggaggcgcgg gatatgtggg aagccatgtt gttcgcagcc tggctcaagc gggtcactcg
     2161 gtgattgttc tcgacaatct gtccactggg catccggagg ccgtggacgt tgctcgaagg
     2221 gtggcgggcg ggggatgcgt ggaacttgtt gttggcgatg ttggcgatgg gacccgggta
     2281 cgcgagcttc tcgtctcgaa ggagatcgac gctgtcatgc atttcgcagc agccagttta
     2341 gtgggagaat ccatgcgcaa cccgtcgaag tattggcgca acaacgttgg gtgcgccgtc
     2401 gagctcttag atgcaatggc ggaggctaaa gtcgataaga tagtcttttc gtcgagtgcg
     2461 gccgtatatg gggagcctac tacaacgcct atccgcgaag accatcccaa agctccaacg
     2521 agcacgtatg gcatgacgaa gctcgccata gaacacatgc ttatcggcta cgaccaggct
     2581 tatgggataa ggtccatatc ccttcgctat tttaacgcgg cgggcgcgca tggctctgga
     2641 gagatggggg aggcccatga ccctgaaacc catctcatcc ccatcatcct ttcggctgct
     2701 ctaggcattc gtgagaacat aagcgtgttc gggactgact acccgacccc agatgggact
     2761 tgtatccgtg actatatcca cgtagacgat cttgccgaag cgcatctttc tgcgatggac
     2821 gcgctgtcgc gcggggccgc aactacggcg tacaatcttg gcaacgggtc aggtttttct
     2881 gtgctcgagg ttcttcggga agcagaacgc gcagtggg
//
LOCUS       DAOSAI010000316         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_22100_length_2907_cov_3.366059, whole genome shotgun sequence.
ACCESSION   DAOSAI010000316 DAOSAI010000000
VERSION     DAOSAI010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22100_length_2907_cov_3.366059"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLM83_09425"
     CDS             complement(<1..357)
                     /locus_tag="PLM83_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK71659.1"
                     /translation="MSKSCRWSICLAVIFLIGLSTYVNAAASKITLNFIGMDQAGMTV
                     DEQKTVIREFEKLHPNIKVNATFVSYDALHDKLVTALAGGGRAFDVMLVDNTWLAELA
                     SAGWLLDVTDRVTPEMK"
     gene            complement(651..1709)
                     /locus_tag="PLM83_09430"
     CDS             complement(651..1709)
                     /locus_tag="PLM83_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOK71660.1"
                     /translation="MVPRERVLAALRRQVPDRVPMYMEFTPPVVDKFHSITGSSDPAD
                     YFDLDIREVSFEPCKKVADFSMYLGELPPGSNVNEWGVGEVKGSGWSHFVDFVHPLQK
                     AATVKEIEDYPFPDMTQEYRWQHIPKAVKRWHELGYAVRGLPPFYGGTVFETAWALRG
                     LENLLVDFVQNRAMAGALLDRVTDISKGTATILAAAGVDVLVTGDDVGMQRTMMMSPS
                     MWREWLKPRLASVIEAAKEANPEVLIFYHSCGYIEPIIPDLIEIGVDILNPVQPESMD
                     PERIKKLYGNELAFWGTIGTQTTLPFGTPDEVRQEVKRMIETVGDGGGLLISPTHVIE
                     PDVPWENIMAFVDAVKEY"
     gene            complement(1767..2681)
                     /locus_tag="PLM83_09435"
     CDS             complement(1767..2681)
                     /locus_tag="PLM83_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK71661.1"
                     /translation="MYRKKQPSRMSRGGLGVTPETGRVAAGDVGARIVRIIGWILIVG
                     WSIFSIFAFAWVVMTSLKSNHELFTNVWSLPKEPQWSNYVKVWTLSQLGTGFANSMLL
                     VLSSVAVLVVVASPAAYVLARKPFKGSDALTNFFTFGMGIPSQLLLVPLFFLMFKMKL
                     VDSLFGLAIVYVALSLPFTIFYLVGFFRTLPGELEEAAMIDGCTSFQAFSRIMLPLAS
                     PGVLTVSIFNFLFLWNDFLLALTFLSSNDKFTLSLGLFGLKGTMQYTGDWVGLFAGFT
                     LIMLPILIVYLFLSNQMVEGLTAGATKG"
BASE COUNT          766 a          825 c          630 g          686 t
ORIGIN      
        1 cttcatctcg ggggtaactc tatcagtaac atccaataac caaccagcag atgcgagttc
       61 tgcaagccag gtattgtcaa caagcattac atcgaatgca cgtccacctc cggcaagcgc
      121 tgtgacgagt ttgtcatgaa gagcatcata cgatacaaaa gtagcgttca ccttgatgtt
      181 tgggtgcaac ttttcgaact cgcgaataac ggttttttgc tcatccacgg tcattccagc
      241 ttgatccata ccaatgaagt tgagtgtgat cttcgatgca gcagcattta catatgttga
      301 caaaccaata agaaaaatga cagctagaca aattgaccat ctacagctct tgctcataga
      361 gtggaccctc ctcacggaaa ttagtgatac cgtctgctat gacctgctgt cctgtcaaac
      421 tccactgcct taacacacgg attgcctatc gcgtcccccc tcaacttgta cgcatatgcg
      481 tatatgttag aaacatttct acgcgctgcc acgaattcct gcttcaaaaa caaaagggaa
      541 tatcagcgat gccctgcagt cagtgtctgc agggcatcgc agcatcgctt tgctggacta
      601 atagccgacg agtgttgggg cgcgaaaggc gcctttacgg gtctagcccc ttagtattct
      661 ttcacagcat ccacaaaggc catgatattc tcccatggga cgtccggttc gattacgtga
      721 gtcggagaga tgagaagtcc tccgccatcg ccgacggttt ctatcatcct cttcacttcc
      781 tgcctcactt cgtcgggagt cccaaacggc agggtggtct gggttccgat agtgccccaa
      841 aaagctaact cgttgccgta aagcttcttg attctttccg ggtccatgct ctccggttga
      901 acgggattga gaatgtctac tccgatctct atgagatcag gtattatcgg ttctatgtag
      961 ccgcaactgt ggtagaagat aagtacctcc ggatttgcct ccttcgccgc ctctataacc
     1021 gacgccaagc gtggcttcag ccactcgcgc cacatcgatg ggctcatcat catcgtcctc
     1081 tgcattccaa catcatcacc agtcacaagc acatcgacgc cggctgctgc aagaatggtg
     1141 gcagtgcctt ttgatatgtc cgtaactcta tccaacagtg cgcctgccat cgcccggttc
     1201 tgaacaaagt cgaccaagag gttctctagc cctcgaagtg cccatgcggt ttcaaagacc
     1261 gttcccccat aaaacggagg cagtccgcgt acagcgtatc ctagctcgtg ccaacgcttc
     1321 actgccttag gaatgtgctg ccacctgtac tcttgagtca tgtcggggaa tgggtagtcc
     1381 tcgatctcct ttaccgttgc cgctttctga agcggatgga caaaatctac gaagtgagac
     1441 catccagacc ccttgacttc accaacaccc cactcgttaa cgttgctgcc aggcggaagc
     1501 tcccccaaat acatagaaaa gtccgcaact ttcttgcagg gctcgaagct cacttctcgt
     1561 atgtctaaat caaagtagtc tgcaggatcc gaagatccgg taatactatg aaacttgtcc
     1621 accaccggag gcgtaaactc catatacatt ggcactctgt ctggcacttg tcttctgagt
     1681 gcagcgagaa ctctttccct cggaaccatg ctgtgtgatc cccccagaaa tcttggacaa
     1741 aggcaacgtg gcgcatgtct tggtcatcaa ccctttgtgg cgccagcagt caagccttca
     1801 accatttggt tagaaagaaa cagatagaca atcaatattg gaagcattat cagagtgaat
     1861 cctgcaaaaa gccccaccca atctcctgta tactgcatcg tccccttcag cccgaatagc
     1921 ccgagcgaca gtgtaaactt gtcattcgaa ctcaagaaag tcaacgccaa caggaagtca
     1981 ttccacagga acaagaagtt gaatatcgat acagtcaaaa cccctggtga tgcaagtgga
     2041 agcattatcc tgctaaaagc ctgaaaggag gtacagccat ctatcattgc agcttcctca
     2101 agctccccgg gaagcgtcct gaagaagccc actaggtaga atattgtgaa cggaagagaa
     2161 agagcgacat agacgattgc caacccgaat agggaatcaa cgagcttcat cttgaacatc
     2221 aggaagaaca ggggaaccaa gagaagttga ctgggtatac ccatgccaaa tgtgaagaaa
     2281 ttggtcaatg catcgctccc cttaaacggc ttcctggcta acacgtaggc tgccggcgag
     2341 gccactacca ccagaactgc aacggatgat agaaccagca gcatgctgtt ggcaaacccc
     2401 gttcccaatt ggctgagcgt ccacaccttt acataatttg accattgtgg ctcttttggc
     2461 agactccaca cattggtaaa tagctcatga ttggacttaa gcgaggtcat gaccacccat
     2521 gcaaacgcga atatagaaaa gatcgaccat ccaacaatca gtatccatcc tataatccta
     2581 acaatccggg ctcctacatc tccagccgcg actcttcccg tttccggcgt gacacccaga
     2641 ccgcccctac tcatccgact cggctgcttt ttacggtaca tgtcaatcct cctcgggaca
     2701 atgtcaaccc aagcgccatg tttgaacacg cacgaccttc cacattctca gtattccacg
     2761 ctctcgcgcc tcatgagact tctcacaatg agcaccatga ggatgaccat aatcagcatc
     2821 accacaccca tggcggtact gtaccccatg cggtacacag gctctcgggt tccaaatgcc
     2881 atcacataca tgtaaacggc caggttt
//
LOCUS       DAOSAI010000317         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_22666_length_2854_cov_4.166131, whole genome shotgun sequence.
ACCESSION   DAOSAI010000317 DAOSAI010000000
VERSION     DAOSAI010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22666_length_2854_cov_4.166131"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..103)
                     /locus_tag="PLM83_09440"
     CDS             complement(<1..103)
                     /locus_tag="PLM83_09440"
                     /inference="COORDINATES: protein motif:HMM:NF025386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HOK71662.1"
                     /translation="MAVRFKKSKKIGKYGRINLSKSGVGASIGVPGFR"
     gene            324..698
                     /locus_tag="PLM83_09445"
     CDS             324..698
                     /locus_tag="PLM83_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71663.1"
                     /translation="MSIATGNKFYTPYLLYNVQAETSVFMACPLCQNPKDQTLGFPSA
                     PHLELPLSSRVISVEKEYARSERPRPEPSPISPGNLPFLPASNHLAEAGRNTRADAVV
                     LFCWGLRILSPPPAGIDRHPKG"
     gene            704..1300
                     /locus_tag="PLM83_09450"
     CDS             704..1300
                     /locus_tag="PLM83_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK71664.1"
                     /translation="MYTDLSDEQLIELHLSGDPRGMEAIYDRYFDRIYRLAYSKLGNA
                     PDAEDVTSAVFMKLCRSLGTFRGDSKLSTWIYTVSNNAITDFLRKKRPALSLDADMMM
                     DDGDSVPREIKDDSRGPEELACEDDFARYVMSKLEGLPPHQRTVIELRYFMELSYQEI
                     ADQLGIELGTVKSRLNRAIGALRSMCSSDSEVNANAMR"
     gene            1287..2078
                     /locus_tag="PLM83_09455"
     CDS             1287..2078
                     /locus_tag="PLM83_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71665.1"
                     /translation="MQCDDLGHKNKRIDASTPTGFSHECFEDDATCEKDLAIIEALHS
                     GIAATPEFRASLKQEFMAEARADAARHAESAAGMNKPASEKPRKKSGSKVRPLYARYA
                     AVAAAAIVIISIFTTNWLKPARPDVTQLADAPSRRNAASIEERQDELAYGEGNIRGML
                     AVTSDIKAMGFAPQAGDSAAAVEVSLKSALTDDSLQELSAIANQAGGSVALSGLDAVA
                     SMSIPSEVLDHVLSNLDASLGIVDVSRPVEINTAKTCITLYFTAD"
     gene            complement(2065..>2854)
                     /locus_tag="PLM83_09460"
     CDS             complement(2065..>2854)
                     /locus_tag="PLM83_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71666.1"
                     /translation="VVRTALFTPVLCAVDVGVETTLGHSSAGTPGVLGGRLWLVRRDG
                     VWVVVLGQAFSKARPVSSHWSMVGDELGNAWRWVGRGGWLASGSSAQVVWDGNNGDAG
                     AARGVLEAAARWRDTSIIDFGEHLSTYAKGRIEYVNNANLEDEGGASKDTGAFAGGSS
                     GCDCKQSTASVDGGHDRMNSGAWQGDQEDGSLEAIGWAQFLSGRKAQNWAWSVRCDVH
                     SRTLATVTFSSAISLDEVPITSNFTRLTMVLEDGEWKVASVSLQ"
BASE COUNT          701 a          891 c          685 g          577 t
ORIGIN      
        1 cccgaaaacc cgggacacca atagaagcgc cgacccccga tttgctcagg tttatcctgc
       61 cgtatttgcc gattttcttc gactttttga acctgaccgc cactacactc cctcctagcc
      121 aaaaatcccg actattagca ggcgcctcag cttgcgccaa caccgcttcg cttcaaaatc
      181 ccgcccgcct ttcgccttgc cttcccctct ataattatag aatgaacgca aaagcccttc
      241 agcaatcctg cacaaacctc tcaccgtgtt tcaacctaaa ggagaataag cccgatggcc
      301 aacactagga cgcagccaac ctaatgagca ttgctaccgg aaacaagttc tacactccgt
      361 acctgcttta caatgttcag gccgaaacct ctgtcttcat ggcatgtccc ctttgccaaa
      421 accccaaaga tcagacgctc ggatttccct ccgccccgca tttggaactt cctctttcaa
      481 gccgcgtcat atcggttgag aaggagtatg cccggagcga aaggcctagg cctgaaccgt
      541 cgcctatctc ccccggaaac ttgcccttcc tacctgccag caatcatttg gctgaggcag
      601 gcaggaacac cagggcggat gccgtagtac tattctgttg gggattgcgg attttatcgc
      661 cacccccagc aggaatcgac aggcatccta aggggtgagc caggtgtata cggatctctc
      721 tgatgagcag cttatagagc ttcacctttc gggcgacccg agaggaatgg aagcgatcta
      781 cgatagatac ttcgatcgca tatacaggct ggcctactcg aagctgggaa atgcccccga
      841 cgccgaagac gtaacatccg ccgtttttat gaaactatgc agatccctcg gcacgttcag
      901 gggtgactca aagctctcga cgtggatata cactgtatcg aacaatgcga taaccgactt
      961 tctgagaaaa aagcgtccgg ctctgtcgct ggacgcagac atgatgatgg atgacggtga
     1021 ctctgtgccg cgcgagatca aagacgactc ccgcggaccc gaagaactgg cgtgcgagga
     1081 tgatttcgcc cgctatgtaa tgagcaaact tgaggggctt ccgccccatc agcgtacagt
     1141 cattgagctc aggtatttca tggaactgag ctatcaagag atcgcagacc aactcgggat
     1201 cgaactggga acagtgaagt ccaggttgaa tagggcaatt ggcgccctgc gatccatgtg
     1261 ttcctctgac agtgaggtga atgcaaatgc aatgcgatga tctgggccat aagaacaaga
     1321 gaatagatgc atccactccg acgggctttt cgcacgagtg cttcgaagac gacgccacat
     1381 gcgaaaagga cctcgctatc atcgaggccc ttcactccgg tatagcggca acccccgaat
     1441 ttcgggccag cctcaaacag gagttcatgg cagaggccag agccgatgca gccaggcatg
     1501 cagagagcgc agccggaatg aacaagcctg catcggaaaa gccccgtaaa aagagcggca
     1561 gcaaggtcag gccgctttat gcgagatatg cagctgtagc cgcagccgct atcgtaatca
     1621 tatcgatttt caccacgaac tggctgaagc ccgcgcgccc tgacgtgaca cagcttgcag
     1681 atgccccgtc ccggagaaac gctgcctcca tagaagaaag gcaagatgaa ttggcatacg
     1741 gcgaagggaa cattcgaggc atgctagccg tgacgtccga tatcaaggca atgggctttg
     1801 cgcctcaggc aggcgacagc gcagcggcgg tagaagtcag cctcaaatcg gcgttgacag
     1861 atgactcgct ccaagagctt tcggctattg ccaaccaagc aggcgggtca gtggctttat
     1921 ccggtctaga tgcagttgca tccatgagca tcccctctga ggtgctcgac catgtcctgt
     1981 cgaacctcga tgcaagcctc ggaatagtgg acgtgtcaag gcctgttgag atcaacaccg
     2041 caaaaacatg tatcaccctg tatttcactg cagactgaca gacgccacct tccactcgcc
     2101 gtcctctaga accatcgtga gcctcgtgaa gttgctcgtt atgggcactt catcgaggct
     2161 tattgccgac gaaaaggtga ccgtcgccaa agtacgactg tgcacatcac acctgacgct
     2221 ccacgcccag ttttgggcct tccgtccgct taaaaactgc gcccagccta tcgcttcaag
     2281 gctcccatct tcctgatcac cttgccatgc accagagttc attcggtcat gaccgccatc
     2341 gaccgatgcc gtactctgtt tgcaatcaca gccagaactt cccccggcaa aagccccagt
     2401 atccttgctc gctccacctt catcctccaa attggcgtta ttcacatact ctatcctgcc
     2461 ctttgcataa gtcgaaaggt gctccccaaa gtcgataatg ctcgtatccc gccagcgcgc
     2521 agcggcctcc aacacgcccc gggccgcgcc cgcatctcca ttattgccat cccaaaccac
     2581 ctgcgcagaa ctgcccgaag ctaaccaccc tcctctacct acccaacgcc aggcgttgcc
     2641 cagttcatcc ccgaccatcg accaatggga agagaccgga cgcgccttcg agaatgcctg
     2701 gcccaatacg accacccata ctccgtccct acgaaccagc cacagtctgc ctcccagcac
     2761 tccgggggtt cccgcagaag aatggcccag agtcgtctca accccgacat cgaccgcaca
     2821 caacaccggc gtgaaaaggg cggtgcgaac caca
//
LOCUS       DAOSAI010000318         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_22819_length_2842_cov_3.916756, whole genome shotgun sequence.
ACCESSION   DAOSAI010000318 DAOSAI010000000
VERSION     DAOSAI010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22819_length_2842_cov_3.916756"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PLM83_09465"
     CDS             <1..1208
                     /locus_tag="PLM83_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71667.1"
                     /translation="DVEIIWEAIPPKNLRGMPLPEELWSVSEDRLSAVLFGSAPGRFE
                     ITVRAERSPAPEEDQLPEIGDGCLPFAQIRLAARAVDPWPMGAASGSTPTKEAMNRLA
                     LVDDFEGRTSWTGAASSTAVRSVVTTANVEGPTPDFPAPTVDQVDNLATPSGAVIVLE
                     YDLRSPEQTRAAYIRPSSPVALPSETSAIRVWVYGDGAGHWLRATVQDLLGQETPIDF
                     PRISWIGWRVVEAPIPQTLVPPLCLSQVYVVEFKPELQAAGRIAIDNIAAVLAPTVPQ
                     PAGQAVDTLLCSAPGRQIVRIDGVRLPLPWPDLLAEFEQFRLGNDLELVVVLRGTNAV
                     DIDTLGVGAALTDPLEAKLLLEMLKGLADGTTSVVLVQDAPTDRKLTSDMEAMIDGVK
                     VIWRKSSPQ"
     gene            complement(1480..1872)
                     /gene="rpsI"
                     /locus_tag="PLM83_09470"
     CDS             complement(1480..1872)
                     /gene="rpsI"
                     /locus_tag="PLM83_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOK71668.1"
                     /translation="MAQLQYAATGRRKCSVARVRLTPGTGKITVNGKPSEEYFGRQTL
                     ELLAREPFEVANRMDRYDAIADVSGGGLSGQAGAIRHGIARALCEGEPELRPVLKKAG
                     LLTRDSRMKERHKYGLKKARKAPQFSKR"
     gene            complement(1898..2335)
                     /gene="rplM"
                     /locus_tag="PLM83_09475"
     CDS             complement(1898..2335)
                     /gene="rplM"
                     /locus_tag="PLM83_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOK71669.1"
                     /translation="MSRTYTPKAEEISRKWYVVDAEGKPLGRLASEVAQILKGKHKPV
                     YSPHMDMGDHVIVINADKVVLTGNKRQTKIHYWHTGYPGGIRQMNDEEFLSTKPEKAV
                     EKAIVGMLPKNRLGRQMAKKLKVYAGPEHPHAAQMPEPLEITV"
     gene            complement(2447..>2842)
                     /locus_tag="PLM83_09480"
     CDS             complement(2447..>2842)
                     /locus_tag="PLM83_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71670.1"
                     /translation="NPLTDEAVQAMAEAGVALVGRHDFSAYAASGSSARTSVRTIHRL
                     EVNTDRWESLGLNLVVVEIEADGFLYKMARTIVGALLEVGMGRMDSEGPGRLLELRDR
                     KLGPATAPADGLYLMKVAYPEPIGGLASP"
BASE COUNT          635 a          866 c          707 g          634 t
ORIGIN      
        1 atgacgtcga gatcatctgg gaggcaattc ccccaaagaa tctacgcggc atgccactcc
       61 cagaggagct gtggtccgtg tcggaggatc ggctctccgc tgtcctattc ggcagcgcgc
      121 caggccgatt cgagatcaca gtcagggctg aacgatcgcc agctcccgaa gaggatcagc
      181 ttccagaaat cggcgacgga tgtttaccct ttgcccaaat acgtctggct gcccgcgccg
      241 tcgacccctg gcccatgggc gcggcaagtg gctctacgcc gaccaaagag gcaatgaacc
      301 gccttgcgct agttgacgat ttcgagggcc gcacctcatg gactggcgca gcatcttcca
      361 cagcggttag gtcagtcgtg actactgcaa acgtggaagg acctacaccc gactttcccg
      421 ccccaaccgt ggaccaagtt gacaatcttg caactccctc gggcgctgta atcgtccttg
      481 agtatgatct gaggagcccg gaacagaccc gcgcagcgta catcaggcct tctagtcccg
      541 tagcactgcc ttcggaaaca tcagccatac gggtgtgggt atatggggat ggtgcaggcc
      601 actggcttcg cgccacagtc caagatttac tgggacagga aacccccatc gattttccgc
      661 ggattagctg gataggatgg agggtcgtag aagcgcccat tcctcaaacg ctagttccgc
      721 cactctgcct ttcccaagtg tacgttgtcg agttcaagcc cgagcttcag gcagcaggaa
      781 gaatagccat tgacaacata gctgccgtct tggccccaac cgtccctcaa ccggccggtc
      841 aggctgttga cacacttctc tgttcggccc ctggtcgaca gatcgtgaga attgacgggg
      901 tgcgattgcc gctgccatgg cccgatcttc tggccgaatt tgaacaattc cggttaggta
      961 atgacctgga actagtggta gttttgagag ggacaaatgc cgtcgacata gatactctcg
     1021 gcgtcggtgc ggcactcaca gaccccctcg aggcaaaact cctgctggaa atgttgaagg
     1081 gcttagctga tggcacgacc tcggtggtgc ttgttcagga tgctcctacc gataggaaac
     1141 tcaccagtga catggaagcc atgatcgacg gggtgaaggt catctggcga aagtcgtcac
     1201 cccaatgaga aaacgaatct aataccggat gagatagcct gaaaaaatgg aacgtatcgc
     1261 cgtcgctatc atctccacta caacaaggcg aaggcctttt tgcagcattg tatcggacgt
     1321 aactaatacg ctaaataccc ccgcaaatat gggattatgc agtaatgcaa aagaggaacc
     1381 agccacaacg actcgttcct ctggcttttt atggtcagcc acgggccaag acggcccgcg
     1441 ggcatgacca aatagagtac aatatcgaac cgacgccttt tacctcttgg aaaactgagg
     1501 cgcctttcgg gccttcttca ggccgtactt atggcgttct ttcatgcgcg aatcacgcgt
     1561 gagaagccct gctttcttaa gaacgggcct cagttcaggc tcgccctcac aaagcgccct
     1621 ggctattcca tgacgtatcg ccccagcctg cccgcttagc ccgccacctg acacgtcggc
     1681 aatggcatca tatctgtcca tcctattcgc gacctcaaac ggctcgcgtg caagcaactc
     1741 cagggtctgg cgtccaaaat actcctcact tggtttgccg ttgaccgtga tcttcccggt
     1801 tcccggcgtc agccgcacac gggcaactga gcacttgcgc cttcccgtcg cggcatactg
     1861 caattgcgcc acagagtaat cccccttcct cgcccggtta taccgttatt tcgagcggct
     1921 caggcatctg cgccgcatgc gggtgttccg gtcccgcata gactttgagt ttcttagcca
     1981 tctgccgtcc aagcctgttc tttggaagca tcccaacgat cgctttctcc accgctttct
     2041 caggcttagt cgaaagaaac tcttcatcgt tcatctggcg aatcccacca ggatatcccg
     2101 tatgccaata gtgaatcttg gtctgacgct tgttccccgt caggactacc ttatcagcgt
     2161 tgatgactat cacatgatcg cccatgtcca tatgaggaga gtagaccggc ttatgcttgc
     2221 ccttgagtat ctgggccact tcgcttgcaa gcctgcccaa gggctttccc tcggcatcca
     2281 caacatacca cttgcgcgat atctcctccg ccttaggcgt gtatgtgcgt gacatcaggt
     2341 tccctcccag cttcaactgc tcgtctgagt gcaatcagtg gttccggggt ttggcgccac
     2401 tggtacaaaa ctcaccaagc aattttaata tagccaaaat agactgtcaa ggcgaggcga
     2461 gaccacctat gggttctggg taggctactt tcatcaagta gagtccgtcc gccggagcgg
     2521 tcgcaggccc gagttttcga tctcgcaatt caagcaatct tcccggaccc tccgaatcca
     2581 ttctgcccat cccaacctcc aaaagcgctc ctactatagt acgcgccatc ttgtataaga
     2641 acccatccgc ctcgatctcc acaaccacaa gattcaaccc gagagattcc catctgtcgg
     2701 tattaacctc aagtcgatga atagtgcgca cagaagtcct tgctgaggac ccggatgcag
     2761 cataagccga gaaatcgtga cgccccacga gcgccacacc ggcctctgcc atcgcctgca
     2821 ccgcctcatc agtcagcgga tt
//
LOCUS       DAOSAI010000319         2801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_23261_length_2801_cov_3.518208, whole genome shotgun sequence.
ACCESSION   DAOSAI010000319 DAOSAI010000000
VERSION     DAOSAI010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23261_length_2801_cov_3.518208"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLM83_09485"
     CDS             complement(<1..361)
                     /locus_tag="PLM83_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOK71671.1"
                     /translation="MARGAMFGAITAVLAHISFPVPFSPVPVSGQSFGAMLAGLLLSP
                     KAAAASQLVYLLAGAVGLPVYAGGHAGIGVLLGPTGGYLWGMAAGAYVSSWIVKMHIS
                     KGNGLTWQKCAIASAIGG"
     gene            complement(509..1054)
                     /locus_tag="PLM83_09490"
     CDS             complement(509..1054)
                     /locus_tag="PLM83_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOK71672.1"
                     /translation="MIEPGRALAISIDFQDKILATVEDRDEIIAQATSFVKVARLAGM
                     PVLRLEQNPDRLGTTTDLMRDALGDSPVFPKMTFSALRSPEIESAVRQSQAEQLILLG
                     VETHICVAQTALDAMAIGYEVYVLADASSSVKSFDRKTAIERMRHAGAVISTAQAAAF
                     EIVGAAGTPLFRQAKSLLKEM"
     gene            1490..1978
                     /locus_tag="PLM83_09495"
     CDS             1490..1978
                     /locus_tag="PLM83_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71673.1"
                     /translation="MQTENREKPMPGPRRGKAGSVLGWTVLLIVLVIAVFAVAGELIF
                     RHGAVPLPAGAKLQHISSVRLRDLDGDFEVVAIDVGKVHGVQPALEISISFWSMMADF
                     EISLVGPNDDVMWREEYNAKMARVSTPKISFETAARGQWRLILSGRARNLMLSVTWKI
                     DE"
     gene            2585..>2801
                     /locus_tag="PLM83_09500"
     CDS             2585..>2801
                     /locus_tag="PLM83_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOK71674.1"
                     /translation="MLYVPNDSNDPRFNLAFEEYVLKNFDMDNSYLLLWRNAPTVVVG
                     RFQNTPDEVNEDYVRKHGVHVVRRMTGG"
BASE COUNT          658 a          678 c          829 g          636 t
ORIGIN      
        1 caccgccgat tgctgaggcg atggcacatt tttgccatgt cagcccgttc ccttttgaaa
       61 tgtgcatctt aacaatccac gaggagacat aggcgcctgc cgccattccc caaaggtacc
      121 ctcctgtggg ccctagaagc accccgatgc ctgcgtggcc tcctgcatag acgggcagcc
      181 ccactgcgcc tgcgagcaag taaacgagct ggcttgctgc cgcggctttg ggtgaaagca
      241 gcaggccggc caacattgct ccgaaggatt gtcccgagac cggaacaggg ctgaatggga
      301 ctggaaagga gatgtgggcc agtactgctg tgatggcgcc gaacatagcg cctctggcca
      361 atgattttat ctcatagctt ttccccatag attgcataga tctatcacat gccttgatga
      421 gtagtgcaat aaggtggtga gggacgagcc tgcccccgtt tcccattcta tcccgatgcg
      481 actagctgaa tcaaccgagc gctactgctt acatctcttt taaaagagat tttgcctgcc
      541 tgaacagagg cgttcccgcg gcccccacta tttcgaacgc cgctgcttgt gctgtagata
      601 tcacagcacc cgcgtggcgc atccgttcga ttgcggtctt cctgtcgaat gattttactg
      661 aggatgaggc atctgctagg acgtacacct cgtatccgat ggccattgcg tcgagagcgg
      721 tctgagccac gcagatatgg gtctccacac caagaaggat caattgctca gcttgacttt
      781 ggcggacagc tgattcgatc tcaggggatc tgagagcgct aaaggtcatc tttggaaaga
      841 caggtgaatc cccaagagca tcccgcatga gatcggtggt ggtgcccaaa cgatcgggat
      901 tctgctccaa gcgcagaacg ggcatgccgg ctaggcgggc cactttcaca aaactggtag
      961 cctgggcaat gatctcatcg cggtcttcga ctgtggccag aattttgtcc tggaagtcga
     1021 tggagattgc caaagctcta ccgggttcta tcattcgagg cctccatttc aaatccaact
     1081 ctaacacccc cgtgcacaga tattcggcgc cgaggattcc tctccttcga aggagtgcgt
     1141 tggaatcgcc aatggactgg ttagaagtga gcgggattcg ggggtacaca taagatagta
     1201 aggaggagaa agatcgcaaa ggataaaatt gagtcatgtg gaaccattac gggtgcataa
     1261 tgggatgaga agccagcggt agcgggcact tggcggtcat acgaggcttg cgatttcgct
     1321 gcccattcgg ctaaggggtc ggcggctgat attggggatc ttcgattctg ggttgggccg
     1381 ggtgtgttta tggagacagg catggcggca cctggttgtc tttgggcatt gccggtcgat
     1441 atgtggatgg atgaggtttt gccgtataga tccacgggag gggtaaaaaa tgcagacgga
     1501 gaatcgcgaa aaacctatgc cggggccccg aaggggcaaa gcagggtctg tgttgggctg
     1561 gacggtgttg ctgatagtac tcgttattgc agtctttgcg gtcgcaggcg agctgatctt
     1621 ccggcatgga gcggttccgt tgcccgctgg tgcaaagcta cagcacatat catctgtgag
     1681 gctgcgggat ctcgatgggg attttgaagt cgtagctatt gatgtcggca aggtgcacgg
     1741 ggtgcagccc gctctcgaga ttagtatcag tttttggtct atgatggcgg atttcgagat
     1801 ctccctcgtt gggccaaatg acgatgtgat gtggcgggag gaatacaatg cgaagatggc
     1861 cagggtgagc accccaaaga tatcgtttga gacagctgcc cgcggccagt ggcgtctgat
     1921 cctctccgga cgggcaagga acctcatgct gagcgtgacg tggaagatcg atgagtgact
     1981 tcgggaagat attgtgaagg cgttatttcc gcgaaggact atgcccaata caggagaggc
     2041 ccgtctgcag caaaaaagca gaatcgggcg ggctcaatac ggcctcgaaa aaggggtgca
     2101 ggagttgaca tatgactaca gcgagatcag gactaaactc gtccgtgaca ggtttaggga
     2161 gacaagtttt gagaatcgag attacatcga tgcactgact ggcggtaatt tgctatcctc
     2221 tcccgcaggt ttacgatgga gcatcagtgc atcgagaagg agacgtggga gaaggagaaa
     2281 aaacgcgggt aaaagcgatt gcggaatcaa cggaaggagc ttggcggatt gtgctgtggg
     2341 caaatggcga ggacctctca cctcactcca aaaggcgtat gatatccatt caatcacctc
     2401 acttcatctg tcctgtctga accgtaactt tcatcattga tgctgcttca gtttgtaggc
     2461 gcgagaccct gggcaatcgg caaatggcgt ttcggcccaa tcaagtggtc aagctctcaa
     2521 ttgcgggcaa tgcaaactcg gggatcgcgc aggaacttat cgagacttac ttaggaggat
     2581 cgcgatgctt tatgtaccga acgattcaaa tgacccacgc ttcaatctgg cctttgaaga
     2641 atacgtgctg aagaacttcg acatggacaa ttcatacctt ctcctctggc gcaatgcccc
     2701 tacagttgtc gtcggccgtt tccagaacac gccggacgag gtgaacgaag actatgtcag
     2761 gaaacacggg gtgcacgtcg taaggagaat gaccggcggc g
//
LOCUS       DAOSAI010000320         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_23377_length_2792_cov_2.974059, whole genome shotgun sequence.
ACCESSION   DAOSAI010000320 DAOSAI010000000
VERSION     DAOSAI010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23377_length_2792_cov_2.974059"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLM83_09505"
     CDS             <1..783
                     /locus_tag="PLM83_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71675.1"
                     /translation="SAITAEFSQHLNCSAFPLVKEYAERKQQHYSDTVDRLSKSKDAL
                     LNNIRELESRRSQADAARQQAEQDAGLAGLEKGREEDGYNGDGNSRLHGQGSNYREAE
                     RKERAARETASLQKVRLDSTTTELASARADLSQLEQRLAAATQAHSQEAIRLDQALLA
                     DPRYFRGANDLLSLRLALQKLRQDPLRGELVNQHTLELEVLVIFLDLLLLFIKWAPHN
                     ESYSARLAASRRLEIARAVREANQQVSRMGQQRPNIRIVREP"
     gene            1333..2394
                     /gene="tssA"
                     /locus_tag="PLM83_09510"
     CDS             1333..2394
                     /gene="tssA"
                     /locus_tag="PLM83_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HOK71676.1"
                     /translation="MTIESWLQPVSEDLPCGPNLEDDLDFLSLEESSREKRSEEFRKD
                     DGDTIQTPKSKIDWFVVHSLAEGLLTRSKDLRVAIYLMRAALHIRGFTGIVDGLALIK
                     ALLERFWDDVHPQLEADSDNDPTMRINALVPLASNDALIGDLRSSYLLNSRSEGQLTV
                     REIEVAQGLLAEGTSGTRLSESQLHGLLSAAIAENPDLPQQASEAQTSLKNILQIVDE
                     HIGSSYTPDLKPLQYILNAIIRAIREVRPAVAEKEALASLALASGGSPLSTPSAGGLH
                     ASGLHEIRSRQDVLQILDRLCDYLARTEPTNPVQIVLRRAQRMMNMTFLELMQDIAPD
                     GLEQAQIVVGEKLNPDSEE"
     gene            2451..>2792
                     /gene="tssB"
                     /locus_tag="PLM83_09515"
     CDS             2451..>2792
                     /gene="tssB"
                     /locus_tag="PLM83_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HOK71677.1"
                     /translation="MMSGSSQKFIARNRAPRVQIEYDVELYGAEKKIQLPFVMGVLSD
                     LSGHPSEPLAPVADRKFLEVDIDNFDSRMKAMKPRLAFQVPNTLTGEGNLNVELAFES
                     MDDFSPAAVARK"
BASE COUNT          679 a          743 c          766 g          604 t
ORIGIN      
        1 tcggcaatca cagccgaatt ctcgcagcac ttgaattgtt ctgcgtttcc cttggtaaag
       61 gagtatgccg aacgcaagca gcagcactac tcggatacgg tggacagact gagcaaaagc
      121 aaggatgccc tcttgaacaa catccgggaa ctggaatccc gccgctcaca agctgatgca
      181 gctcggcagc aagcggaaca ggatgcagga ctggccggtc tggaaaaagg ccgcgaggaa
      241 gatggttaca acggcgacgg aaattcccgt ctacatggcc agggcagcaa ttacagggag
      301 gcggagcgga aggaaagggc cgcccgggaa acggcctcac ttcaaaaagt ccgcctggac
      361 tctaccacca ccgagttggc gagtgcccgc gctgaccttt cccaactgga acagcgccta
      421 gcagcagcca cacaagccca tagccaggag gcaatccgtc tggatcaggc tctgttggct
      481 gacccgcgct acttccgcgg tgcaaacgat ctgctctctc tgcggctggc cttacaaaaa
      541 ttacggcagg accctttgcg gggtgaacta gtaaaccaac acacactgga gttggaagtg
      601 ctcgtgatct ttcttgacct gctgttgctc ttcattaaat gggcgcccca caacgaatcc
      661 tacagcgccc gtctggctgc gtcccggcgg ctggagatcg cccgggcagt aagggaggcc
      721 aaccagcagg tttcacgcat ggggcaacaa cgaccgaaca tccggattgt gcgggagcca
      781 taaaatcaca aatagatatt ttttgcggca cggtcatggc ctgaacatga gaggcaacgg
      841 ggaccggtct atatctcacc ctcgtacagg ctggcaactg tagttttagg atcaagagcg
      901 gacaggagtc tgctcgagct tacaagtagt catcataacc acttgagagt tggagatttt
      961 ctggcgacgt ttacgacact tgcgtgcgca atcgccctga ttttccttat tgggttcact
     1021 tcttcgcaaa gttggcgcat tatgctgacc aagggagagt aaaccgattt cagcaagggg
     1081 aactgaaggg tgtggccagc aaggagttgg cgattccgcc gtttcttgcc ggaccaaagt
     1141 atctccggtt taaagccgaa ttcatttccc gacgctcatg gcagtaacat cccgattggg
     1201 gtcatgacga ccctgccgct tctggtttac ttgttcattc ctgcccacat ggcagagaga
     1261 aggccagtgt agtttatttt ttaatgattt tggcgcgcag cctgcagttc gtctggatat
     1321 agcgaggact cggtgacaat agaatcatgg ttgcagccgg tttctgaaga tcttccttgt
     1381 ggccccaatc tggaagacga tctcgatttt ctcagtctcg aagaatcgtc cagggaaaaa
     1441 cgcagcgagg agtttcgcaa agacgacggc gatacgattc aaactccaaa gtcgaaaatc
     1501 gactggttcg ttgtgcactc attggcagaa ggtctattga cgcggagcaa ggatctgcgc
     1561 gtggccatct acctgatgcg ggcggccctg catatccgcg gtttcaccgg catcgtcgat
     1621 ggcttggctc tgatcaaagc cttgctcgaa cgtttctggg atgacgtgca tccacaactg
     1681 gaagccgata gtgacaacga tccgaccatg cgtatcaatg cgctcgtacc gcttgcctcc
     1741 aacgatgcgc tgattggcga tctacgctcg agttatttgt tgaattcccg tagcgaaggt
     1801 caactcaccg tccgtgaaat cgaagtggcg caagggctgt tggcagaagg aaccagtggc
     1861 acgcgacttt ccgaatcgca actgcacgga ttgctttctg ctgccatcgc tgagaacccg
     1921 gacttgcccc aacaagccag cgaggcccaa accagtttaa aaaatattct ccaaatcgtt
     1981 gatgaacata taggcagttc atacacaccg gatttgaagc ccctgcaata catcctcaat
     2041 gccatcatcc gagccattcg cgaagttaga ccagcagtcg ccgagaaaga ggccttggcg
     2101 agcttggctt tagcaagtgg gggttctcca ctcagtacac cttccgcagg tggtttgcat
     2161 gctagcggtt tgcacgagat tcgttctcga caagacgtgc ttcaaatact tgaccgatta
     2221 tgcgattatc tggccaggac cgaaccgacc aatccggtac aaatcgtctt gcgccgtgcg
     2281 cagcgcatga tgaacatgac cttcctggaa ctgatgcagg atatcgcgcc ggacggcctg
     2341 gagcaggcgc aaatcgttgt cggcgaaaaa ctgaatccgg atagtgaaga ataagcagtc
     2401 agggtcggac aaactgttcc ggcaaaggta taacttgtca tctggagaca ataatgtcag
     2461 gtagcagcca gaagttcatc gcccgtaacc gggcgccgcg ggtacagatc gagtacgacg
     2521 tggaactcta cggtgcggag aaaaagattc agcttccctt cgtcatggga gtgctgtcgg
     2581 atctctcggg ccacccgtcc gaaccgcttg ccccggtggc cgaccgcaaa tttctggaag
     2641 tggatatcga caatttcgat agccggatga aagcgatgaa accgcggctg gctttccagg
     2701 tgccgaacac actgactgga gaaggcaatc tgaatgtcga gctggctttc gaatcgatgg
     2761 atgacttctc accggccgct gtcgcccgca ag
//
LOCUS       DAOSAI010000321         2789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_23404_length_2789_cov_2.817849, whole genome shotgun sequence.
ACCESSION   DAOSAI010000321 DAOSAI010000000
VERSION     DAOSAI010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23404_length_2789_cov_2.817849"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PLM83_09520"
     CDS             complement(<1..820)
                     /locus_tag="PLM83_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71678.1"
                     /translation="MRSVQELLADLARLEGGRFDETLFELVRRGPKALDRLFAYIDSD
                     APPSISAKVNESIGRQWTRVQEVLIELPPKAFSSLGVKALIERGPSDEIARRAAKCIE
                     RGMIETAACIATVLSSSENACIERAILEARLWMAAGRPKRAMARLSPYCGPGEAGTMV
                     SEWMVAAAMAAGEPRRAFLAMPVREPDVTQASHAVGDMIDACSVEDLAECIFDVARRG
                     DLAGWELIGAAAEEAWERAVQDDADVALGLAGARSEIEGFLDGIESVLWDLESCP"
     gene            complement(840..1277)
                     /locus_tag="PLM83_09525"
     CDS             complement(840..1277)
                     /locus_tag="PLM83_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK71679.1"
                     /translation="MNEIGVLLREAREASGMSLEEVSEATKIRSKYLEAIEQGDFAVI
                     PGEVYLKGFMRNYADCVGLDGGQVVARYNELSLLKETAALKQLEEERLERLQKSKEEA
                     RSRRAKTILRGLVTAAIGVAAVAGIYYAARAFGFVTWKPTWLP"
     gene            complement(1298..>2789)
                     /locus_tag="PLM83_09530"
     CDS             complement(1298..>2789)
                     /locus_tag="PLM83_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOK71680.1"
                     /translation="MAELLESTLASFGVEASVVGISAGPVVTRFEVQPGPGVKVSKIV
                     NLADDLSLAFASAGVRIEAPIPGKAAVGIEVPSKNRSVIYLREVIESDEFQKAESRLT
                     FAVGKDIAGKPIVADMADLLHLLVAGATGSGKSVCLNSIICSLLFKATPDEVRFLLID
                     PKRVELSMYDGIPHLLAPVVTDAKKASGYLRWVVEEMENRYRLFQIAGARNIGQYNEM
                     VRLGIADEYIDADANIAGSDVGTDRNIEGSEADAQGGSDVSTEANKGKAAGPRLKPLS
                     YIVVIIDELADLMLVAQREVEDAIARLAFMARAAGIHLIIATQRPSVDIVTGIIKSNI
                     PSRIAFAVSSQVDSRIILDSSGAEHLLGKGDMLFHPVGLPKPIRVQGAYISDEEVASL
                     VRYLKQQGKPIYEAKAVETTGSVGEGIAEDDELFPEAVRLVVSSGEASISKIQRRFRV
                     GYARAARLIDTMEVLGIVGPYEGSKPRKVLITEEDLEDALGNRGLV"
BASE COUNT          595 a          906 c          651 g          637 t
ORIGIN      
        1 ctggacatga ctccagatcc caaaggacac tttcgattcc atcaagaaag ccttcgattt
       61 ccgatcgggc gccagccaac cccagagcaa catctgcatc atcctggacg gccctttccc
      121 atgcctcttc tgccgccgcg ccgatcagct cccatcccgc aagatctcct cggcgtgcca
      181 cgtcaaagat gcattccgct agatcctcga ctgaacacgc gtcgatcatg tcgcctaccg
      241 cgtgcgaagc ctgtgtgaca tcgggttcac ggaccggcat cgccaggaat gcgcgtcgtg
      301 gctcccccgc tgccatggca gcagccacca tccattctga aaccattgtt cccgcctctc
      361 cgggcccgca ataagggctt aaccgagcca tagcccgctt tgggcggcct gccgccatcc
      421 acaaacgagc ttccagaatt gcccgctcaa tgcaggcgtt ttccgacgag gagagaacag
      481 tggcaatgca cgcggccgtc tctatcatgc cccgctctat gcacttcgcg gcacgccttg
      541 cgatttcgtc agatggccca cgttcgatca gagctttcac gcccagcgaa gaaaatgcct
      601 ttggcggcag ctcgatgaga acctcctgaa ccctcgtcca ttgccttcca atagattcat
      661 tcactttggc tgagatgctt ggaggcgcgt cagaatcgat gtaggcaaag agcctatcga
      721 gggctttggg accgcggcga accagttcga agagggtctc atcaaaacgg ccgccttcga
      781 gcctggccaa atcggccagg agttcctgca cactcctcaa ttcccttcat ccctccaagc
      841 tatggcagcc atgtcggttt ccacgtcaca aacccgaatg cacgtgccgc ataatagata
      901 cctgccacag ccgccacccc tatggcagct gtaaccaaac cgcggagaat cgttttcgcc
      961 cggcggctcc tcgcctcctc cttgctcttt tgcagccgct ccaggcgctc ttcttccagc
     1021 tgctttagtg cggctgtttc cttgaggagg ctaagctcat tgtatctcgc cactacctgc
     1081 ccgccgtcga gccccacgca atccgcgtaa ttgcgcataa aacccttgag gtatacttca
     1141 cccggtatga ccgcaaagtc tccctgctct atggcctcca agtacttcga cctaatcttg
     1201 gtcgcctcgc tcacttcttc caggctcatg ccactggcct cgcgtgcctc cctaaggagc
     1261 acaccgattt cgttcaacgg caacaccccc gcgcccctca tactaacccg cgattgccga
     1321 gcgcatcttc aagatcctcc tcggtgatga ggactttccg cggcttgctt ccctcatagg
     1381 gtccgacaat gccgagcact tccatggtat ctatcagcct tgccgccctt gcataaccca
     1441 ctctgaaccg acgttgtatc ttcgatatag acgcctctcc tgaactgacg acgagcctga
     1501 cggcttcggg gaaaagctcg tcgtcttcag cgatgccttc ccccacgctg ccggtggttt
     1561 ccacagcttt ggcctcataa ataggctttc cctgttgttt caagtatctg accaggcttg
     1621 ccacctcttc gtcggaaata taggcgccct gcacccgtat gggctttggc agtcccacag
     1681 gatgaaacag catatcgcct tttcccaaca gatgctccgc gcctgaagaa tctaagatga
     1741 tcctggagtc cacctgcgag gaaacggcaa acgctattct cgacggtatg ttcgacttta
     1801 taattccggt cacaatgtca actgaaggcc tctgcgtcgc aataatcaga tgaattccgg
     1861 cagcccgcgc catgaaagcc aatcgagcaa tagcgtcttc cacttcacgc tgggcaacga
     1921 gcatcaaatc agcaagctcg tctatgatca cgacgatgta cgagagaggc ttcagccgtg
     1981 gccccgcagc ctttcctttg ttggcttcag tggagacatc tgaaccgcct tgcgcgtccg
     2041 cctctgatcc ctcgatattt ctgtctgtgc cgacgtcaga tccagcgata ttggcgtctg
     2101 catcgatata ctcgtctgca attcccagac gtaccatctc gttgtactgg ccaatattcc
     2161 gcgctccggc tatttggaag aggcggtacc tgttctccat ttcctccacc acccatctga
     2221 ggtagcccga agccttcttc gcatctgtca ccaccggagc taagagatgc ggaatcccgt
     2281 cgtacataga aagctccact ctcttgggat caatgagaag aaacctgacc tcatccgggg
     2341 ttgctttaaa cagcaggctg catatgatcg aattcaggca tacgctcttg ccagaaccag
     2401 tggcccccgc aaccaagagg tgtaggagat cggccatatc agccactatt ggctttccag
     2461 caatatcctt gcccactgca aaggtgaggc gggattccgc cttttggaat tcgtccgact
     2521 ctattacttc acgcaagtat attacagacc tgtttttgct cggaacctcg atacccacag
     2581 cggccttgcc cggtatcgga gcttctattc tgacacctgc cgaggcaaag gccaatgaca
     2641 ggtcatcagc gaggttaacg attttcgata ccttgacacc gggacctggt tggacttcaa
     2701 aacgcgtgac aaccggcccg gcgctgattc cgactacaga agcttccacg ccaaagctgg
     2761 caagagtaga ctccagcagt tcggccatg
//
LOCUS       DAOSAI010000322         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_23431_length_2786_cov_3.767484, whole genome shotgun sequence.
ACCESSION   DAOSAI010000322 DAOSAI010000000
VERSION     DAOSAI010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23431_length_2786_cov_3.767484"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLM83_09535"
     CDS             complement(<1..805)
                     /locus_tag="PLM83_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK71681.1"
                     /translation="MPKKVRLPIVLMLAFALALGLAGAPGAAKVAYEPWGPHVDEIIM
                     PIIKESEARRIAFERGESVVWAGLTKPEDIDRAKVIPNADMTMTLGFHMFYLCFNMRE
                     YPLADQAVRQAIAHCTDRDNIIRTLFKGYMLPMTSFIPQVSPFYKADVPVYPFSHAKA
                     AEVLDKAGYKLDPVTKKRIDPKTGKPMRELKIFTPTYEVAPTSAELGKMIADSCQKVG
                     LPVVPEPMDFPVMLDKLDIMEFDMYCLAWNLSRNPTSLYSFFHSSQDVEA"
     gene            complement(1130..1549)
                     /locus_tag="PLM83_09540"
     CDS             complement(1130..1549)
                     /locus_tag="PLM83_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71682.1"
                     /translation="MKMSGGLNPFLIGAIGFAICISSTVGLISIILREYLPVPLWVAL
                     VPAAVAFASLGVASPEVPCMVVLTGTELAIAPLAASLVVGFPASRYQSALVVESAFRL
                     AIAADLSIMIPLAFLSMTAGSAIRGLWIGAGPPPWSG"
     gene            complement(1546..2070)
                     /locus_tag="PLM83_09545"
     CDS             complement(1546..2070)
                     /locus_tag="PLM83_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71683.1"
                     /translation="MGLPHYLRRAEAISGTMAVVAMVASAALIWAIYPLYSDYVRWTE
                     AAAAEVIAASVTLCGDTGTICIDAGFKSPSALFAAEIEALEFNVDCQGRHCGFYRIRV
                     PDDSGILLRDVDNEVLLQLAAPVAPENCGQLSRNGAAVLSGNLIVRIFLPHAEHVARF
                     SLEGELGNVGSDVQ"
     gene            complement(2078..>2786)
                     /locus_tag="PLM83_09550"
     CDS             complement(2078..>2786)
                     /locus_tag="PLM83_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71684.1"
                     /translation="RQRVQMIFQDPYESLDPRMTVFDIISEPLNVNHIGNLEEREERV
                     AYMLSRVGITPPEMFFFRFPHELSGGQRQRVAIARALVVNPEFIVADEPTSMLDVSIR
                     TGVMKLLAELQEDFGMSYLYITHDLAVARYLCDRIAVMYLGKIVEMAGAEDIIKNPLH
                     PYSKALISAVPLPDPAIRRDVPNIKGGVSKPINPPPRCRFYPRCPVAKNICRDSDHPE
                     LCEASPGHFVACHLVAR"
BASE COUNT          693 a          800 c          687 g          606 t
ORIGIN      
        1 cggcctcgac gtcctgcgac gaatggaaga agctgtatag gcttgttggg ttcctgctaa
       61 ggttccaggc caggcaatac atgtcgaatt ccattatgtc aagtttatcg agcatgactg
      121 ggaagtccat cggctcgggc acgacgggaa ggccaacctt ctggcatgaa tcggcaatca
      181 ttttgccaag ttccgcagag gtcggagcaa cctcgtacgt aggcgtgaag atcttcaatt
      241 cacgcatggg tttgcctgtc ttgggatcta ttctcttttt agtgaccgga tcaagcttgt
      301 aaccggcctt gtcaaggacc tcggcagcct ttgcatggct gaacgggtag acaggcacgt
      361 cagccttata gaaaggcgac acctgtggaa tgaaactcgt cataggaagc atatagccct
      421 tgaagagcgt gcggattatg ttgtcgcggt cggtgcagtg tgcaatagcc tgacgtacag
      481 cttggtcggc caatgggtat tccctcatgt tgaagcaaag gtagaacatg tgaaaaccaa
      541 gagtcatagt catgtcagca ttggggatga ccttcgccct atcgatatcc tcaggcttgg
      601 taaggccagc ccacacaaca ctttcgccgc gctcaaacgc gatcctccgg gcctcggatt
      661 ccttgataat gggcatgata atctcgtcaa cgtgggggcc ccatggctcg taagccacct
      721 tggcggcgcc aggcgcgccg gctaggccaa gggcaagcgc aaatgccaac atcaatacaa
      781 tcggcaagcg tacctttttc ggcattgagc atgtacctcc taaaaatgat tggtcgtgtt
      841 actcggtctc caattctctc tacactacga cgggatcgta aagggaatcg aattgcccgc
      901 caatccctcc tctcttgaac ccaacaacga ttgcaaaaac gcactgtcgt caattctttt
      961 ggcagttaag atgatatcca caagtttcat tcgacaccaa gtatacgaat tccttcttcg
     1021 attttacttt acgctcccag cgatccccca ccacaattgg cttgccttcc ttttctggct
     1081 catgcaaaat ccattccgct tttcaaggac tgccatgtgc ccaatggcat cagccactcc
     1141 acgggggagg accagcgcct atccataacc cgcgaatcgc cgaacctgct gtcatcgaca
     1201 aaaaggctaa gggaatcatg atggaaagat ctgctgcgat tgccaagcgg aaagcagact
     1261 caaccactaa ggctgactgg tatcgtgacg caggaaagcc cactaccagt gaagcagcca
     1321 aaggcgctat cgccagttcc gtgcctgtca gcacaaccat gcatggcacc tcgggcgatg
     1381 caacccccag ggacgcaaac gcaaccgcag ccggcaccag agcaacccag agcggcaccg
     1441 gcagatactc gcggagtatt atggatatca aacccacggt ggaggagatg catatcgcga
     1501 accctatagc tccgataaga aacggattga gccctcccga catcttcatt gaacatcgct
     1561 ccccacgttc cccaattcgc cctcaagaga gaaccgggcc acatgttcgg cgtgcggcaa
     1621 gaagatgcgg acaatgagat ttccgcttag aaccgcagct ccgttccgac tcaattgtcc
     1681 gcaattctcc ggcgcaacag gcgcagccaa ttgcagtaat acctcgttgt cgacgtcgcg
     1741 cagaagaata cctgaatcat caggcacccg aatcctgtag aatccgcagt gcctgccttg
     1801 gcagtccaca ttgaactcca aggcctcgat ctcggccgca aacagggcgc tcggtgattt
     1861 aaaccccgca tctatgcata tggttcctgt gtccccacaa agcgtgacgg aggctgcaat
     1921 aacctccgcc gctgctgctt ctgtccatct cacataatca ctgtagagag ggtagatcgc
     1981 ccaaataagt gctgccgaag caaccatggc cactactgcc atcgtcccgg atatggcctc
     2041 agcccgcctt agataatgcg gcaaacccat accgcagtca cctcgccacc aaatggcagg
     2101 ccacaaaatg tccaggagac gcctcacaga gctcaggatg gtcactgtct cggcagatgt
     2161 tcttcgctac aggacaacgc ggatagaacc tacacctcgg aggagggttt atgggcttac
     2221 tcactccgcc tttgatgttc gggacatcgc gccgtatcgc cggatcaggc aggggcacag
     2281 ctgaaatcag cgccttcgaa taggggtgaa gcgggttctt gatgatatcc tcagctccgg
     2341 ccatctccac aatctttccc aaatacatca ctgcaatcct atcgcacaaa tatctggcaa
     2401 cagcgagatc atgagtgatg tatagataac tcataccgaa atcctcttgc agttcagcga
     2461 ggagcttcat gactcccgta cggatcgaca catccagcat ggatgtgggc tcatcagcta
     2521 cgatgaattc gggattgacc acgagagcgc gagcgatcgc cacccgctga cgctgaccac
     2581 ccgagagttc atgcgggaac ctgaagaaaa acatctcagg gggcgtaatg cccacacgag
     2641 acaacatgta ggcaaccctc tcctcccttt cctccaagtt tccgatgtgg ttcacattga
     2701 gtggttcgga tattatgtcg aataccgtca tccttgggtc gagcgattca taggggtcct
     2761 ggaatatcat ctggaccctc tgtctg
//
LOCUS       DAOSAI010000323         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_23432_length_2786_cov_3.556573, whole genome shotgun sequence.
ACCESSION   DAOSAI010000323 DAOSAI010000000
VERSION     DAOSAI010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23432_length_2786_cov_3.556573"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLM83_09555"
     CDS             <1..281
                     /locus_tag="PLM83_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HOK71685.1"
                     /translation="ALCPGLLDEYIEDKSLYELLRTRYHIDPIWAKQSFEASLCKSRE
                     AELLGIRRGAPVLRVERIAYDSSETPIEYTASVYRGDRYKLHVYMNTR"
     gene            392..1021
                     /locus_tag="PLM83_09560"
     CDS             392..1021
                     /locus_tag="PLM83_09560"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOK71686.1"
                     /translation="MAEGRILHYFGPGHSHMLGEELFYRAGGLAPVNAILEPALMVNY
                     GAGKSSKMERLPGLAKIVLDESSVKSGDVIIVASTSGVNQVPVEMACEAKAAGVKVVA
                     LTAVEYSRATPLRNDRNVRLCEVGDVVIDTLVTYGDAAIEVDGVAQKIAPVSTVIGAA
                     IVNAITIGVVENLVARGIDPPIFKSANIPGGMEHNAKLLERYRPMIKSL"
     gene            1050..2036
                     /locus_tag="PLM83_09565"
     CDS             1050..2036
                     /locus_tag="PLM83_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOK71687.1"
                     /translation="MKEDLVAALDGGGTKTVLVIADSCGQVLGIGKGGPVNALFVPDS
                     TAIESVQNAAKGAMERAGLSDEADAPVHVRAVYASVPGASREIIEAGLSGLVAYDVLQ
                     VEGDDYATFRGAFPEGYGVVVLAGTGSFAMGRSSDGHTLTAGGWGPLLGDEGSGYAIG
                     LAGLRAVALASEGRCEATVLMDMAMEMFGVERARDIIKAGLTRERIAGFAMAVSSAAA
                     QGDDVACRILADAGADLGRLGGHIITGLGMKGQGCPVALSGGASKAGAPLRDSFAATI
                     KRIDPSCKIADPKLSPAGGALLIAYDLAGIELDWERSNRILACAEGFDELRP"
     gene            2235..>2786
                     /locus_tag="PLM83_09570"
     CDS             2235..>2786
                     /locus_tag="PLM83_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK71688.1"
                     /translation="MGKQADSGLATTSGSPKPSNKRRAALKRELTAWSFVLPSLIGLG
                     IFVYGSLVYSLYVSFTSWDLLTPPKWVGLANYARTFKDPNFIQCMGNTLYFVVAIVPL
                     GIILAMAMAIALNKERPGFKFFRGAYYMPSITSTVAIGMVWLWIFNPDQGVLNTILQF
                     FGVANPPRWLESVKWGKPALSIMR"
BASE COUNT          626 a          654 c          852 g          654 t
ORIGIN      
        1 cagccctttg ccccggactt ttggatgagt acatagagga taaatccttg tatgaactgc
       61 ttaggacgag atatcacata gatcccatat gggccaagca gagtttcgag gcttccctgt
      121 gcaagtcaag agaggccgaa ctgttgggga tcaggcgtgg cgcccctgtc cttcgcgttg
      181 aacgcattgc ctacgattct tcggaaactc ccattgaata tactgcatcg gtatacaggg
      241 gagacagata caagctgcat gtctacatga acactcgttg acgaggtgcg gaggcgaagg
      301 ttggtggatg agtatttcga gcttgtagag gctttgctgt ccagagtcca tcaatcccaa
      361 tgcggcaacg tagagattgg ctgctgattc tttggcagaa ggacgcatat tgcactattt
      421 cggcccaggg cattctcaca tgctaggcga ggagctcttt tacagagcag gagggttagc
      481 tcccgtaaat gccatactcg agccggcgct gatggtcaat tacggtgcag ggaagagctc
      541 gaaaatggag cgtcttcccg ggctggccaa gatcgtcctc gacgaatcgt cagtcaagtc
      601 cggcgatgtg ataatcgtgg cgtccacctc cggagtcaat caggtgcctg tcgagatggc
      661 ttgcgaggca aaggccgctg gtgtaaaggt agtagcgctt actgctgtgg aatactcccg
      721 cgccactccg ctgaggaacg acagaaacgt gcgcttgtgc gaggtcgggg acgtggtaat
      781 tgacacgctt gttacatatg gcgatgcagc tatagaagta gatggcgtgg cccagaagat
      841 tgctcccgta tccacggtaa taggcgctgc aatagtcaat gccatcacca ttggtgtagt
      901 ggaaaacctg gttgccagag gcattgatcc gcccatcttc aagagcgcta acataccggg
      961 gggcatggaa cacaatgcga agctcttgga aagatacagg ccgatgataa agtcgttgta
     1021 ggtacgattc atcttgggag gaacatcaga tgaaagaaga tctagtggca gcgctcgatg
     1081 gcggcggcac caaaactgtc cttgttattg cggatagctg cggtcaggtg ctgggaatag
     1141 ggaagggcgg ccccgtgaat gccctctttg ttcctgatag tactgcaatc gagtctgtgc
     1201 agaatgcggc gaagggtgcg atggagcggg cagggctgtc agacgaggcc gatgcgcctg
     1261 tgcacgtgcg ggctgtttat gccagtgtgc cgggggcgag tagggagata atcgaagctg
     1321 gcctgtcggg tttggtggcc tacgacgttc tgcaggtcga aggcgatgac tatgccacct
     1381 tccgcggagc tttcccggag ggatacgggg tcgttgtcct tgcgggcact ggatcttttg
     1441 cgatgggacg ctcttcggac gggcacacct tgaccgccgg tggctggggc cctctcctag
     1501 gagacgaggg aagtggttat gcaatcggac ttgccgggct tcgagctgtt gcccttgctt
     1561 cggaaggcag atgtgaagct actgtgctca tggatatggc catggagatg ttcggtgtcg
     1621 agcgtgccag ggacattatc aaagctggac tgactcggga gcgaatagcg ggatttgcga
     1681 tggctgtttc gtccgcggcg gcccagggag atgacgtagc ctgcaggata ctggctgatg
     1741 ccggagccga tttgggaagg cttgggggac acataatcac cggtttgggc atgaagggtc
     1801 aggggtgtcc ggtcgccctg tcagggggtg catccaaggc tggagcgccc ttgcgcgatt
     1861 cgtttgcggc tacgataaag cggatcgatc cgagctgcaa gatagccgat ccgaagcttt
     1921 cacctgcagg tggcgcgttg ctcatagcgt atgatttggc aggaattgaa ctggattggg
     1981 agagatcgaa ccgcatcctg gcgtgcgctg aggggttcga cgagctccgt ccgtaaacgt
     2041 ggatccgaag aagggatctt gagttataga tagaaagagt ataataaata taactaggaa
     2101 tgttggcttg cgaataacac agcattaagc cttggggcaa aatccgaggt tatcccggag
     2161 atgctgtagc ttatatatgg tcagtagcga gttgtgatac ttaccgtgca ctcttcttaa
     2221 tggaggttgg ctacatggga aagcaagctg atagtggact ggccactact tccggaagtc
     2281 cgaagcctag caacaagaga cgcgctgccc tcaaacgcga gcttaccgca tggtcttttg
     2341 tgttgccatc actgataggc ctcggcatat tcgtctacgg ctcacttgtc tattcactgt
     2401 acgtatcctt tacaagctgg gatcttctca cccctcccaa atgggttgga ctggctaatt
     2461 acgcgcgaac gttcaaagat cccaatttca tccaatgcat gggaaacacc ctctattttg
     2521 ttgtagccat cgttcctttg gggataatcc tggcaatggc tatggctata gcgctcaata
     2581 aggagcggcc agggttcaag ttcttcagag gggcctacta tatgccatcg atcacgtcta
     2641 ccgtcgctat cggaatggtg tggctgtgga tattcaaccc ggaccaggga gtgctcaaca
     2701 ctatcctaca gttctttggg gttgctaatc cccctcgctg gctggaaagt gtcaaatggg
     2761 ggaaacccgc cttaagcatc atgcgc
//
LOCUS       DAOSAI010000324         2777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_23531_length_2777_cov_3.651359, whole genome shotgun sequence.
ACCESSION   DAOSAI010000324 DAOSAI010000000
VERSION     DAOSAI010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23531_length_2777_cov_3.651359"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLM83_09575"
     CDS             complement(<1..1033)
                     /locus_tag="PLM83_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOK71689.1"
                     /translation="MHKLSEKMNQYIFPALSTYYEEPLVLEKGEGKYLYDDAGRQYLD
                     FFGGILTVSMGHCNPEIISRVVEQVRTLQHTSTVYIIRPMVELAEQIARIAPGKLQKC
                     FFVNSGTEADETAVLLSTIYTGNDEIVALRHAYSGRSALAMSLTAHAPWRQGKALWAN
                     VKHAHSPYCYRCAFGRTYPNCDLECAKDIEELICTSTSGRIAAFIAEPIQGVGGFITP
                     PKEYFAEAVSIIRKYGGLFIADEVQTAWGRTGGKMFGIEHWGVEPDIMTFAKGLANGA
                     PIGAVIATSEVADSWHGPTISTFGGNPVSMVAAQATLDLIVKNDIPAHVEKVGARLRE
                     GLLRLQEKYE"
     gene            complement(1475..2248)
                     /locus_tag="PLM83_09580"
     CDS             complement(1475..2248)
                     /locus_tag="PLM83_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOK71690.1"
                     /translation="MTTDAEKYTVRSVERAFTILEALKDNGEMRLIDVSRSTMINKGM
                     AFKLLYTLCSKGYVEHDGETGKYRLGKTLFELGHAAGNQITERRGAHDILRSLVEKIN
                     ETAHLAVLQGSDVLYLDKVESNASLRMVTAVGIRRPAYCTGTGKALLAGLTDDVVRRL
                     MEGKPMRRFTPNTICDIDDLLAELALVRTRGYALDNEENEPGIMCVGAPVRNRDGVVV
                     SAISVAGPTIRINRETVPQLANEVIKAAERISETLVCRS"
BASE COUNT          644 a          754 c          676 g          703 t
ORIGIN      
        1 actcatactt ctcctggagc ctaagaagac cctcacgcaa tcttgcgcct accttttcaa
       61 catgagcagg tatgtcgttt ttgactatga gatccaaggt tgcctgagcc gccaccatgc
      121 tcactggatt tccgccgaat gtggagatcg ttggaccatg ccaggagtct gccacctcgg
      181 aagtggcaat caccgctccg atgggggcac cattggcaag gcctttggcg aacgtcatga
      241 tatcgggctc gacgccccag tgctcgattc caaacatctt tccgcccgtg cggccccaag
      301 ctgtttggac ttcatctgct ataaagagcc cgccgtactt ccgaatgata gaaaccgctt
      361 ccgcgaaata ctccttcgga ggcgttatga acccacctac tccctggata ggctctgcaa
      421 tgaaagcagc aattcggcct gaagtagatg tgcaaatcag ttcctcgatg tccttcgcac
      481 attcgaggtc gcagtttggg taagtcctgc cgaaggcgca acgatagcag taagggctgt
      541 gcgcatgttt gacatttgcc cataaagcct tgccctgccg ccagggagca tgagccgtca
      601 gggacatggc cagcgcggaa cggccactat aagcatggcg cagcgccaca atctcgtcat
      661 ttcctgtata tatcgtggag agcagaacgg ccgtttcgtc ggcctcagtt ccgctgttga
      721 cgaagaagca cttctgtagc ttgcccggag caattcttgc gatttgttct gccagttcca
      781 ccatgggtct tatgatgtac actgttgagg tatgctgaag ggttcttacc tgttccacta
      841 cgcgactaat gatctccgga ttgcaatgtc ccatgctcac agtgagaatt ccaccgaaga
      901 aatcaagata ctgcctgcca gcatcgtcgt aaaggtattt gccctctccc ttttccagga
      961 ccaaaggttc ctcgtagtag gtagacaacg cagggaagat gtactggttc atcttctcag
     1021 acaacttgtg catagagcat gcctcccaaa ctagatttcg ttctgcgaca gccacacgct
     1081 ggcgcgtaca cggtgacccc gaacgctagt ctgcttcaaa ggacatgcca tccaccctcc
     1141 tctcccgcta cctcagtaat acaacgctcg cctgttcacg aagttaagtg tcaggttcca
     1201 ttcgaagctt cgccgcgaga tgatgccagt atcacttgcc ttgttatgcg aacctcacag
     1261 aaaagcacca aatagtgaca tagccctctc actgaaaccg gatgcagttt ctatatctaa
     1321 actcggtttc tattctggcc aaattcaacg cacattgcac aattccttcc actaaatctt
     1381 gccatatcta caagtaaatc aaggagttgg ggtgttgtag acgagggcaa tgcctatagg
     1441 cgaaggtgtg ctgcaatagc aatgcatgga aaccctaaga cctgcacaca agtgtttccg
     1501 agatgcgctc tgcagctttg atgacttcat tggcgagctg gggaactgtc tcacggttga
     1561 ttctgatagt cggcccagcc acgcttatcg ccgatacgac cacgccgtct ctattgcgta
     1621 cgggggcgcc cacacacatg atgcctggct cgttttcttc attgtctagg gcgtatcccc
     1681 tggtacggac gagagccagc tcagctaaga ggtcatcgat atcacagatg gtgttcggtg
     1741 tgaacctgcg catcggcttc ccctccatta ggcggcggac tacgtcgtca gtcagtcccg
     1801 caagcaacgc cttgcctgtg ccagtgcaat aggcaggacg gcgaatacct actgctgtta
     1861 ccatcctcaa cgatgcattg ctctccacct tgtcaaggta cagaacatca gaaccctgta
     1921 acaccgctaa atgtgctgtt tcattgatct tctcgacgag tgatcgtagg atgtcgtggg
     1981 cgcccctgcg ctccgttatt tgattgcctg cagcatggcc aagctcgaag agggtcttcc
     2041 caagcctgta cttcccggtt tctccgtcat gctccacata gcctttggaa cagagtgtat
     2101 aaagcagctt gaaggccatt cctttgttta tcatagtgct tcgactcacg tctataaggc
     2161 gcatctcgcc gttgtccttg agtgcctcca gaatggtgaa tgctcgctcc actgagcgta
     2221 cagtgtactt ctcggcgtcg gttgtcataa atcagttcac tcctgccttg aggtcctggt
     2281 ctgctaaact acaaatgctc atgcttagca gccaatttga aatctcgaaa ccatattacc
     2341 attttgacgc cacttttgca cctatggagc aagggtgcta aagctcgact cgttctggga
     2401 tcgtgccaca tatagtgttt tggcaggtct aagaacgcca gcatagcgat ggatgcttgc
     2461 aattgcctgg cagtccctga gatgacacag cctaatttgc ccaggagaag aagcaagcgg
     2521 tttgcatgaa cggaacggtt tgtggatcaa cgcaataacg cctggtaaac gtgatgacga
     2581 gaagagactg aagcatcatg tgtcttgcaa agaggtttgc gattgaaaca cggtttcctg
     2641 tcctgaaccc cttgcacagt cttttcagac aggttcttca agtttggcct gtcgatgaga
     2701 taattccacg cggaaaggac ggggccgatc ttcggcaccc ttggacatca aggttttgga
     2761 tttatggatc atagttt
//
LOCUS       DAOSAI010000325         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_23699_length_2764_cov_3.011812, whole genome shotgun sequence.
ACCESSION   DAOSAI010000325 DAOSAI010000000
VERSION     DAOSAI010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23699_length_2764_cov_3.011812"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..967
                     /locus_tag="PLM83_09585"
     CDS             227..967
                     /locus_tag="PLM83_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOK71691.1"
                     /translation="MRAESEVEPMQQTPEQEELAGAPLREFGEIETPVSPPSNIHVLS
                     IIGQIEGHTVLPPQNKTTKYEHIMPQLVAIEQSDEIEGLLILLNTVGGDVEAGLAISE
                     LLAGMTTPSVSIVLGGGHSIAVPIAVSCSYSMIASTATMTIHPLRLSGQLVVGTQQTY
                     EYLERMQERVIKFVSEHSRCSTEGFRDLMFRTGELLRDVGTVLVGQDAVDAGLIDSVG
                     SLADALRKLDELIAVRRKIAGRAKERIH"
     gene            967..1230
                     /locus_tag="PLM83_09590"
     CDS             967..1230
                     /locus_tag="PLM83_09590"
                     /inference="COORDINATES: protein motif:HMM:NF025401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HOK71692.1"
                     /translation="MLYTVMPIEDVLGEPAWQMDPYAAWRASMGPVEPPMLVSCRVRG
                     VPLSVWPMPDGRGQVERVMSTDPADYLDPTISPGSMVDIREYL"
     gene            1314..2330
                     /locus_tag="PLM83_09595"
     CDS             1314..2330
                     /locus_tag="PLM83_09595"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOK71693.1"
                     /translation="MAIPFIGGLALFLLSLVGMSKALGEILGPRTRAALHGASVPAAF
                     VSGVGVTALIQSSTLVSVTLVGLVNSGAMGLAPAFAAILGANVGTTVTAQIAGLRPTW
                     LGWAAAGAGLVLTAWGWVELRGPGAADRVRKETGRMRMLGGAAAVSFSGVILGLELVA
                     EASGAVMDSDTLKVILTWASHKPILAAVVGAAATAAVHSSSVVSALLVSLTREGLVEL
                     EGAVALVIGSNVGTCFTALVAALPADRDSQTLAWANLIFNVLGAAAFLPFIELFCDLL
                     RLLSTDPGAQLANAHALFNIITAAIVLPFSRQFVELIGGRGKERVYIERHPRVVAGNS
                     PRSH"
     gene            2281..>2764
                     /locus_tag="PLM83_09600"
     CDS             2281..>2764
                     /locus_tag="PLM83_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOK71694.1"
                     /translation="MSVIQGLLLGIVQGLTEFLPVSSSGHLVVAQALMGLEPNMTLAL
                     GVHAGTALSVLVLYSGEVWEIIMGFFRGIAKRDGSAKMAICLILTSIPAALVGFAASD
                     AVEHAFGSPLFAGIGFLISGVVLWCADCISSRQKQRKQESINEGNIGYKQALGVGVAQ
                     A"
BASE COUNT          543 a          729 c          857 g          635 t
ORIGIN      
        1 cgaaggcgtg ctggaagttc agtcattgaa agaccggctg aggcatgcaa tatcctcttt
       61 catcttcgaa cgcaccggca ggcgtccaat ggtcattcca gtggtaatgg atgtgtagcc
      121 tgaatgcgcg ggcggtttct tccccgcctt cgcattccga tacataatta ctctgctcac
      181 accctcatac taacgttgac ttcactgacg gaggtgcaaa cggcatatga gggctgaatc
      241 agaggttgag ccgatgcagc agacgccgga gcaagaggag ctggcaggcg ctccgttgag
      301 ggaattcggg gagatcgaaa cccccgtttc tccgcccagc aacatacatg tattgagcat
      361 tattggccag atagagggcc ataccgtgct gccgcctcag aataagacga ccaagtacga
      421 gcacataatg cctcagctcg ttgctataga gcaaagcgat gagattgagg ggcttctcat
      481 tctcctcaac acggtgggag gggatgtcga ggccgggctg gccatatcgg agttgcttgc
      541 agggatgaca actccgtcag tcagcatagt ccttggaggc gggcattcca tcgcggtccc
      601 aattgcagtt agctgcagct actcgatgat tgcgagcact gccacgatga ccatacaccc
      661 gctgcgtcta tcaggccagc ttgtggtggg aacacagcag acgtatgagt acctcgagcg
      721 gatgcaggag cgggtaataa agttcgtctc ggaacactcc aggtgttcaa ctgagggctt
      781 cagggatctc atgttcagga cgggggaact ccttcgggat gtgggcacag tcctggtagg
      841 acaggatgcc gtggatgcag ggctaataga ctctgtggga agcctcgctg acgcgctccg
      901 aaagctggac gagctcatcg cggtcagacg gaagatagct ggcagggcaa aggagcgtat
      961 ccactgatgc tttacacagt catgcccatt gaagatgttc tgggcgagcc tgcgtggcag
     1021 atggaccctt acgcagcctg gcgtgcctcc atgggccccg tagagccgcc catgcttgtc
     1081 tcgtgccgcg tgaggggagt gcccctctcg gtctggccta tgcctgatgg cagaggccag
     1141 gtggaacggg ttatgtcgac tgatccggct gactatcttg acccaacaat ttcacccgga
     1201 tccatggtgg atatacgcga atacctctag cagcctcgcc acataacctc tccggctgcg
     1261 gcatacggtt ttctgactgg atgctcatgt cgtgaggtgg tggcaaaatg attatggcga
     1321 taccttttat agggggtctc gcccttttcc tgctttcact tgttgggatg agcaaggccc
     1381 tcggagagat tttgggcccg cgcacgcgtg cggctcttca tggcgcctcg gtgccggctg
     1441 ctttcgtatc gggcgtgggc gttacagcgc tgattcagag ctcgacgctg gtaagtgtca
     1501 ccctggtcgg cctcgtcaat tcaggcgcga tggggttggc cccggctttt gctgccattc
     1561 ttggggccaa cgtcggaaca accgtgacgg cgcagattgc ggggcttcgc ccgacatggc
     1621 tcggatgggc ggcagccggg gcagggctcg tgctcacggc gtggggatgg gtggagctcc
     1681 gcggtcccgg ggcagccgac cgcgtcagga aagagacagg gcgcatgcga atgctgggcg
     1741 gcgcggcggc agtcagcttc tccggagtca ttcttggcct ggagttggtt gccgaagcca
     1801 gtggtgccgt gatggactcg gatacactaa aggtcatttt gacctgggca tcgcataagc
     1861 cgattcttgc ggcggtagta ggcgccgccg ctacggcggc agtccatagc agttcggttg
     1921 tgagcgcgct tttagtctca ctgaccaggg aaggccttgt cgaacttgag ggggcggtgg
     1981 ctcttgtcat tggaagcaat gttggcacgt gtttcactgc gctggttgca gccttgccgg
     2041 cagatcgcga ctctcagaca ctggcgtggg cgaacctgat tttcaatgtt ctgggggcag
     2101 cagcattcct cccatttatc gaactcttct gcgatttact tcgccttctt tctaccgatc
     2161 ccggagcgca gttggcgaat gcacacgcat tgttcaacat aatcaccgcg gcgatcgtcc
     2221 tcccattctc acggcagttc gtagagttaa taggaggccg cggcaaggag cgagtttaca
     2281 ttgagcgtca tccaagggtt gttgctggga atagtccaag gtctcactga gtttcttcct
     2341 gtatcaagtt ccgggcatct cgtcgtagct caagcgctaa tgggtcttga acccaacatg
     2401 acgctagcac tgggcgttca tgctgggact gcactttccg ttctagtttt gtacagcggc
     2461 gaggtatggg agatcattat ggggtttttc cgcggaatag cgaagcgcga cggtagtgcc
     2521 aagatggcca tatgtttgat cctcactagt atcccggcgg ccttggtcgg tttcgccgcg
     2581 tctgacgccg tggagcacgc gttcggttcg cctctctttg ctggaatcgg attccttatc
     2641 tcgggagtcg tcctatggtg cgccgactgc atatcgtcgc ggcaaaagca acggaaacag
     2701 gagagcataa acgagggcaa tatcggttac aagcaagctc ttggcgtggg cgtcgcacag
     2761 gcgt
//
LOCUS       DAOSAI010000326         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_23700_length_2764_cov_3.007383, whole genome shotgun sequence.
ACCESSION   DAOSAI010000326 DAOSAI010000000
VERSION     DAOSAI010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23700_length_2764_cov_3.007383"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..234
                     /locus_tag="PLM83_09605"
     CDS             46..234
                     /locus_tag="PLM83_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71695.1"
                     /translation="MLLQATLPHIFTVIAAAVGKYGRKITGAEMPVETGEPGVTSLLD
                     AASAQSRSEVRRGRLPLS"
     gene            472..1158
                     /locus_tag="PLM83_09610"
     CDS             472..1158
                     /locus_tag="PLM83_09610"
                     /inference="COORDINATES: protein motif:HMM:NF018247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOK71696.1"
                     /translation="MNKLSPRKIAAIVAAVGLLGLAAVALKQARRPALVVIENEETDS
                     SYQDGSIHGGDCLSCETDVEGPVAGSVTPELDDDLIVIHVAGAVARPEVYSLPHGSRV
                     ADAVRAAGGATSEADLDMVNLALPVHDGEQICIPRKEPTPSGSSEARIPSNRADISAL
                     SPKETLININTASVAELEQLPGVGPVTAAKIVEYRKINGPYSSVNDLLGVSGIGEAKL
                     SKIAPHATVR"
     gene            1159..>2764
                     /locus_tag="PLM83_09615"
     CDS             1159..>2764
                     /locus_tag="PLM83_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOK71697.1"
                     /translation="MALTAGNENPGKHLFGFGTSVTHGNLGACAVAPVIALFLIVGIL
                     LGFREAGFCLRWAWLVLAGIAFARIETLRLSKKRRAVLAWAVLALAGISLGAVAMERT
                     DGMAARSIARYAGRDVMVRGTIRSVWPYVDPAELILDVDTVMDQGSNGSAVGAVRIRP
                     RWGHEEDWVTWDVRVGDIVWARVQVEYADRPMNPGEPDYRMILLQQGIHAVAWAEHDS
                     MVFAGRNGRRPLLLVADAVRRQMVASASAVLDPPERDLLAGMMFGDYRGVDPIILESF
                     RKSGAAHLLAASGLHVGLVAGLFGALVGIVGASPEAADIAAVLFGGIYAVAAGLRASV
                     VRAWLMLTVAVLAKARRRKGSLIHPLAIGASVQLLINPLLLWNAGFQMSYLAVGALAA
                     FAPLATRPRENAPLGLSALIMQVLTVSVGIQIATWPIIANMTGQVCFVGPLTNLAALP
                     LASVVMTAGVVGCAIYPFLPWVGSAIITGVGVVLRVLTHLVGWMSSLPGATVCCRKLR
                     SFELVAYYLMVSVIWWLAWDRFARFRA"
BASE COUNT          533 a          679 c          892 g          660 t
ORIGIN      
        1 cgggcctcat gtaaataccg agttttggaa ccaaaggctt gacatatgtt gctccaagcc
       61 acgttgcccc atatcttcac ggtgattgct gcagctgtag gcaagtatgg gagaaagata
      121 acaggggctg aaatgccggt ggaaacgggt gagccgggtg ttacgagcct actggacgct
      181 gcgtctgctc agtcgagaag cgaagttcgg cggggcagat tgcctttgag ctagagacgc
      241 tccggaggac tttgggaaga gactgtagcc atgtgagctt gatggcaaag gaggcgtggc
      301 gagatgaagg accacgaggt cggcatcttg cctctcgacg ctttggcgcc aaaaatcacc
      361 cagttggctc acccaaaagg gcgaggcggc ggtggtgtcc gaatggtaac atgagtctag
      421 cagctcggcg tttccaccat gaccggtcgg cgctttcaag ggggattgtc aatgaacaaa
      481 ctgagtccac ggaagattgc ggcgattgta gccgcagtgg gcttgttagg gctggcggcg
      541 gttgccttga aacaggcgcg tcgaccggca ctggtggtga tcgagaatga ggagactgat
      601 tcctcctatc aggacggatc cattcatggc ggggattgcc tgtcgtgcga gacagacgtc
      661 gagggtcccg tagctggttc ggtaacgcct gagttggacg atgacttaat cgtaatccat
      721 gtcgcgggcg cggtggctcg tcctgaagtc tactctcttc cccacggctc gcgggttgcc
      781 gacgcggtta gggcggcggg gggagcaaca tctgaggccg atctcgatat ggtcaatctt
      841 gcgctgccgg tacatgatgg agagcagata tgtattcctc gcaaagagcc tacgccatcc
      901 ggatcttcgg aggcgagaat tccttcgaat agagcagata tctcggcgtt gtcacccaag
      961 gaaacattga taaacatcaa cacagcttca gtcgccgagc ttgagcaatt gccgggggtt
     1021 ggtccagtta cagctgccaa gattgtagag tataggaaga tcaatggacc gtattcttcc
     1081 gtcaacgatt tgctcggcgt ctccggcatc ggcgaggcga aactgagcaa gattgctccc
     1141 catgccacgg tgaggtaaat ggcgctcacg gcgggaaacg agaatcccgg gaagcatctc
     1201 tttggctttg gaacatcggt cacccacgga aacttgggag cgtgtgctgt ggcgccagta
     1261 attgccttgt tcctgattgt gggcatactc ttaggattcc gtgaagccgg cttctgtttg
     1321 cggtgggcat ggcttgtttt ggcgggaatt gcgttcgccc gaattgaaac gctcagatta
     1381 tcaaagaaaa gacgagccgt gctggcctgg gcggtcttgg ccttggccgg aataagcttg
     1441 ggcgcagttg ccatggagcg tacagatggg atggctgcaa ggtcgattgc gcgctatgcg
     1501 gggcgggatg tgatggtgcg tggcaccata cgatccgtat ggccgtacgt ggatcctgcg
     1561 gagcttatcc tcgatgttga cacagtcatg gaccagggtt caaatggctc ggcagttggg
     1621 gctgttcgga taaggccccg gtgggggcat gaggaggatt gggttacctg ggatgtgcgc
     1681 gtaggggaca ttgtgtgggc aagggttcag gttgaatacg ctgatcgtcc catgaaccct
     1741 ggcgaacccg attacaggat gatactcttg cagcaaggga tacatgccgt tgcatgggca
     1801 gagcatgatt cgatggtctt tgcaggacgc aatggtcggc gcccgttgct cctggtggct
     1861 gatgcagtgc ggcggcagat ggtcgcctca gccagtgcgg ttctcgatcc gcctgaacgg
     1921 gatctcctcg cagggatgat gtttggcgac tacagagggg ttgatcccat tattctcgag
     1981 agcttcagga aatctggggc tgcccatctg ctggcagcct ccggcctcca tgttggcctc
     2041 gttgccgggc tttttggagc tcttgtcggc attgtaggcg cgtcgcccga agccgccgat
     2101 attgcggcgg tcttgtttgg cggcatctat gccgtggctg ccggcctgcg ggcatctgtt
     2161 gtgcgggcat ggttgatgct gacagtagcg gtcctagcaa aggccaggcg aaggaagggc
     2221 agcctcatcc atcccttagc cattggcgca tccgttcagc tcttgatcaa ccctttgctt
     2281 ctttggaatg cagggtttca aatgtcctac ctcgccgtgg gtgctctcgc tgcctttgcc
     2341 ccccttgcaa caagaccgcg ggagaatgcg cctcttgggc tgtctgcgct aattatgcag
     2401 gttctcacgg tatctgtggg catacagata gcgacttggc ccataattgc caacatgaca
     2461 ggccaggtgt gcttcgtggg cccgttaacc aatctggcgg ctctaccgct tgcatcagtc
     2521 gtgatgacgg cgggcgtggt tggttgcgca atttacccct ttcttccctg ggtgggttcg
     2581 gcaatcatta cgggcgtcgg tgttgttttg cgggtgctta cacatttggt cggatggatg
     2641 tcatcacttc ctggcgcaac agtatgctgt aggaagttgc gaagcttcga actcgttgca
     2701 tactatctga tggtctcggt catctggtgg cttgcgtggg accgttttgc caggttcagg
     2761 gctg
//
LOCUS       DAOSAI010000327         2762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_23717_length_2762_cov_3.213890, whole genome shotgun sequence.
ACCESSION   DAOSAI010000327 DAOSAI010000000
VERSION     DAOSAI010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23717_length_2762_cov_3.213890"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /locus_tag="PLM83_09620"
     CDS             complement(<1..1938)
                     /locus_tag="PLM83_09620"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOK71698.1"
                     /translation="MIRVFAGRVRPQGRRPVRSATVVAALVLCLMIMAFGPADHGANA
                     GVLTWLCPGSCSAGENLAARNVILFIGDGMGPGQIEAARDVSPFMLSMDFMPVMTRIT
                     TNSWGDNLTDSAASSTALATGHKTVNRSLGVLPDGTPVPNIVEAAKESGRYTGLVSTC
                     SITDATPAGFSVHVADRGSGREIAAAMLETGLDLIVGGGASYFLPTLFEKTPPPIEVA
                     RSKGYTVVQNKEDFLNASSVPLLGLFSMTHMDYDVQRNPDEQPSLAEMTAKAIELLSS
                     TEGEGFFLMIEGGRIDHACHNNDAEMLISEMLAFDKAVSVGLEYASGRDDTLVIVTAD
                     HETGGVDVSSGEIQFSTDGHTTEPVFLFAQGKCSELFASASDNTDVAKLMAMAMQVNL
                     GEVYTVEEGLAGATEKEAPIEEDGTDGAVSCVEEQSRASVGLRERPRLEVKPAHRVNV
                     MTYNIHSGFGADLVLDLDRIADLISEQKVDIAGMCEVDCKTGRSGGVDQAAYIASRLG
                     YEHVYGRTIYYGGGQFGNALISRYPIRSWCNHQLPNPAGNEPRAVLEAQVDVDGTILN
                     VFVTHLDVKDETGRRAQVGALLDIASKAVGPSVIMGDFNASPYADEISLALHEFNDTM
                     PTYRVLVNSDELVKEGLFARDY"
     gene            complement(2066..>2762)
                     /locus_tag="PLM83_09625"
     CDS             complement(2066..>2762)
                     /locus_tag="PLM83_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK71699.1"
                     /translation="PPTFFPKNPTLSNFREVFERIPFARYYMNSIITSVIPTVVTVIT
                     CSMAGFGFAKYRFAGRDVLFWVILATMMVPFPATIVPLYVMMAKAGLVNTYAGLIIVG
                     LSSASGIFMMRQFMMTIPDELLDAARIDGASEFRIFNQIVIPLVKPALATQTLFTFTG
                     HWGAYIWPLICVNSDKLRTLPLAIPYFSGQYFSYQNLINAASLMAIAPVLILFLFTQR
                     YFVEGITITGMKG"
BASE COUNT          633 a          817 c          697 g          615 t
ORIGIN      
        1 gtaatccctg gcgaacagtc cctccttgac cagctcatcg ctgttcacaa ggaccctgta
       61 cgtaggcata gtgtcgttga actcgtgcaa cgccaaagat atttcatctg cgtagggaga
      121 cgcgttgaaa tctcccatta tcacgctcgg ccccaccgct ttggacgcga tgtcaagcaa
      181 ggcgccaacc tgcgcacgac ggcccgtctc atccttcaca tccaggtgcg tgacgaaaac
      241 gttgaggatt gttccgtcca catcgacctg agcttcaagg accgccctag gctcgttccc
      301 tgccgggtta ggaagctggt gattgcacca tgaacggata gggtaacgag agattagcgc
      361 gttgccgaac tgtccgccgc cataatagat ggtgcggccg tacacgtgct cgtatccgag
      421 ccttgaggcg atgtatgcgg cttggtctac cccgcccgat cttcccgttt tgcagtcgac
      481 ctcgcacatg cctgcgatat caaccttctg ctccgaaatg aggtcagcaa ttctatcgag
      541 gtcaagcacg agatctgctc caaacccact atgtatgttg tatgtcataa cattgactcg
      601 gtgtgcgggt ttcacttcaa gccttgggcg ctcacgaagg ccgaccgatg cacgcgattg
      661 ttcttcgacg caagagacgg caccgtcagt cccatcttct tctatgggcg cttctttttc
      721 ggtggccccg gctagtccct cttccaccgt gtacacctcg cccaagttga cctgcatggc
      781 catggccatt agcttcgcca catcagtatt gtcagaggca gatgcaaaca gttcagaaca
      841 cttgccctgc gcaaacagaa atactggttc cgtggtgtgt ccatcggtgc tgaactgtat
      901 ctctccgctg gagacatcga ctccccctgt ttcatggtcg gccgtcacta tgaccagggt
      961 gtcgtcgcgt ccagacgcat actccagccc aactgagacc gccttgtcga aagccagcat
     1021 ctcgctgatg agcatctcgg catcattgtt gtggcaggca tggtctatcc tgcctccctc
     1081 tatcatgagg aagaaccctt caccttctgt tgatgaaagg agttctatgg ccttggcagt
     1141 catctcggca agactgggct gctcatcagg atttctctga acgtcgtaat ccatatgggt
     1201 catggaaaac agccctaaaa gcggaaccga cgatgcgttg aggaaatcct ccttgttctg
     1261 gactacggta tatcccttgc tgcgcgccac ctctatgggc ggaggagtct tttcaaagag
     1321 cgtcggcaga aagtaagaag ctcctccccc gactatcaga tctagaccgg tctccaacat
     1381 tgccgcagct atctcacgcc cgcttccgcg atcggcaaca tggaccgaaa accccgcagg
     1441 agttgcgtcc gttatcgaac aggtgctgac caatccggtg tatcgcccac tctccttggc
     1501 agcttccact atgttgggca ctggcgttcc atcagggagc acgccgagcg accgattgac
     1561 cgtcttgtgc ccggtggcaa gagcagtcga actcgcggct gagtcagtaa ggttgtctcc
     1621 ccacgagttt gtagttatcc ttgtcatcac gggcataaaa tccatagaca acatgaacgg
     1681 actcacatcg cgggcagcct cgatctgccc aggtcccatc ccgtctccga tgaacaatat
     1741 gacatttcgt gcggccaagt tctccccggc agaacatgag ccggggcaga gccatgtaag
     1801 cactccggca ttcgccccgt ggtcagcggg tccgaatgcc attatcatca ggcagaggac
     1861 cagcgcagcc acgaccgttg ccgaccttac aggtcgccgc ccttgcgggc gcactctgcc
     1921 tgcaaaaacc cggatcatta gtaatacccc ctcatcataa tagcgtcatg cgatgttcgg
     1981 gcaaggcgcc aaccggggcc attgcgagtc ggccctggaa gaacgtaccg gacgccctgc
     2041 tttctcataa cctgaaactc gccttctagc ccttcattcc tgttatcgtt atgccctcca
     2101 cgaagtagcg ttgcgtgaac aggaaaagga tcaacacagg cgcaattgcc atgagtgacg
     2161 ccgcattgat caagttctga taactgaagt actgccccga aaaatagggg atcgccagcg
     2221 gcaacgtacg cagtttatcc gagttcacgc atatgagcgg ccatatatag gccccccaat
     2281 gccccgtaaa cgtgaaaagg gtctgagtag ccaaagcagg ctttactagc ggaatcacga
     2341 tctggttgaa gattcgaaac tcgctggcgc cgtcgatgcg cgcagcatcg agaagctcgt
     2401 caggtattgt catcatgaac tgtctcatca tgaatatccc cgacgcactc gaaagaccca
     2461 ctatgatcag acccgcgtat gtgtttacca gcccggcttt ggccatcatc acgtaaagcg
     2521 gcactatcgt ggctggaaaa ggaaccatca tggtagcgag tatgacccag aacaacacgt
     2581 cccttccagc aaacctgtac ttcgcgaacc cgaagcccgc catggaacag gttatcaccg
     2641 tgacaaccgt gggtatcacc gacgtgatga tgctgttcat gtagtaacgg gcaaacggta
     2701 tcctctcgaa aacctctcgg aaattcgaca aagtagggtt cttcggaaag aacgtcggag
     2761 ga
//
LOCUS       DAOSAI010000328         2734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_24044_length_2734_cov_3.206047, whole genome shotgun sequence.
ACCESSION   DAOSAI010000328 DAOSAI010000000
VERSION     DAOSAI010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24044_length_2734_cov_3.206047"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLM83_09630"
     CDS             complement(<1..544)
                     /locus_tag="PLM83_09630"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOK71700.1"
                     /translation="MITNLKPYPAYKDSGVEWLGKVPEHWVVRRLKNAARVIMGQSPS
                     SNDCSVERIGLPFLQGCAEFGVEYPQPKQFCKAPSKVSPAGAILMSVRAPVGRLNTAD
                     QEYGIGRGLCAIVPHPGLYHSRFIRFGVIVCLEGLAVLSTGSTYDAVSVADVAGLRIP
                     LPPLPEQALIVRFLDWTERRL"
     gene            complement(541..>2734)
                     /locus_tag="PLM83_09635"
     CDS             complement(541..>2734)
                     /locus_tag="PLM83_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOK71701.1"
                     /translation="LNELLSLFNIWQSDPEAKFPRGLTPLKSLFRYHLRTIRPWDAHD
                     RIKDTGLWEDWKERIGYGQEKVPFEVELWYRNAPERRQTTESNLEKLVHTLGGEITSQ
                     CVIPEIAYHGVLGRIPIKEVSQFISQVDTLAEFRLFQCEDIMYVHPVGQCAIRIPEDM
                     SETQHIGELVDRVLPSGEPIVALFDGLPLTKHRLLDGRLIVDDPDGFESAYQAKERVH
                     GTAMASLICHGDLDDHEAPLERPVYVRPIMQPRRGFDGRFIEEVIPEGMLPIDLIHRA
                     VRRLYESDGGEGPVAPSVRVINLSIGDRSKPLDRMMSAWARLLDWLSWKYNILFVVSA
                     GNHPHAIELDVPRTHFPNLTADEREKAVIKALASNTRHRRLLSPAETINGLTIGSTHA
                     DASSPSQHFNLIDPFTRMNIPSVYSAHGPGYRRSIKPDILLPGGRQFLREKLGNTHRN
                     AVLETTSFDSLPGQCVAAPGAQGRLDRTWYMRGTSNAAALASRWANRLYEVLERLRKE
                     FHAQIPSEYDTVLIKTLLVHGADWADIRNLYESCLRNPQNSRTFREYVTRFIGYGFAD
                     VTKVIACTDQRVTIMGFGKLEDGQADEFRLALPPSISARNVKRRLTITLAWLTPVNSR
                     HHRYRIAHLWFDPKNSVVPDRIGADGRAVQRGTVQHEVLEGEQAADFQDGDAIVIKVN
                     CRADAGDIPESIRYGLAVTLEVAEDIAIYQEVRERLYVRIPVHGGSTT"
BASE COUNT          642 a          751 c          661 g          680 t
ORIGIN      
        1 tcaacctcct ctctgtccaa tcaagaaaac ggacgataag ggcttgctcg gggaggggag
       61 gcaagggaat gcgcagaccc gcgacatcag ccacactaac tgcgtcataa gttgatcctg
      121 ttgaaaggac tgcgagcccc tccaggcata caatcacacc gaatcggatg aagcgcgagt
      181 ggtataagcc tggatgcggg acaatcgcgc agagccccct tccaatacca tattcttggt
      241 ctgccgtatt cagccgaccg actggtgcgc gaaccgacat gagaatggcc ccagccgggc
      301 tcaccttaga aggcgctttg cagaactgct ttggttgcgg atactcgacc ccgaactctg
      361 cgcacccctg caggaatgga agcccgattc tctctacgct gcagtcattc gaggacggag
      421 actgccccat gatcacccgg gcagcattct tgagcctcct caccacccaa tgctctggca
      481 ctttcccaag ccattcaacg ccagagtctt tatatgcggg gtagggtttg aggttggtaa
      541 tcatgtggta cttcctccgt gcaccggaat tcgaacatac agtctctcgc gcacttcttg
      601 atagattgct atatcctccg caacttccaa tgtcactgcc aatccatagc gtatagactc
      661 tgggatatct ccagcatcgg cacggcagtt caccttgatt acgatggcat ccccatcttg
      721 aaagtctgcc gcctgctcgc cttcaagcac ctcgtgctgg acggtacctc gttgtacggc
      781 gcggccatca gcacctatcc ggtctggtac aacactgttt ttaggatcaa accacagatg
      841 agcaatccgg tacctatgat gtctactgtt caccggagta agccacgcca aagtaatcgt
      901 caaacgccgt ttgacgtttc ttgctgaaat actcggtgga agtgcaagac ggaattcatc
      961 ggcctgtcca tcttctagtt tcccgaaacc cataatggtt acgcgctgat ctgtacaagc
     1021 tattaccttt gtgacatctg caaaaccata acctataaat cgggtaacat actctctgaa
     1081 tgttcggcta ttttggggat tacgcaggca tgactcatac aaattcctaa tatccgccca
     1141 atccgcacca tgtacgagaa gggtctttat taacactgtg tcgtattccg acgggatttg
     1201 tgcatggaac tctttgcgta gccgctcaag aacctcatac agccgattag cccaccgcga
     1261 ggccaaggcc gcggcattgc tcgtgcctct catgtaccat gtgcgatcta acctgccctg
     1321 cgctccagga gcggcgacac actgtccggg aagcgagtca aaagaagttg tttccagaac
     1381 ggcgttgcga tgcgtattgc ccaatttttc gcgaaggaat tgcctcccac cgggcaaaag
     1441 aatgtctggt ttgatgcttc tacggtaccc aggtccatgg gcgctgtaca cgctcgggat
     1501 attcattcgt gtaaaagggt ctatcagatt gaaatgttgc gatggtgatg aagcatctgc
     1561 atgggtagat ccaattgtaa gcccgttgat cgtctcagca ggcgatagca gacgacggtg
     1621 ccgtgtgttt gatgcaaggg ctttgatcac agctttttct cgctcgtcag cggtcaggtt
     1681 tggaaaatgt gtgcgtggga catccaactc aatagcatgg ggatggttgc ccgcgcttac
     1741 cacgaatagg atgttatact tccacgaaag ccagtccagc aggcgtgccc acgcactcat
     1801 catgcgatcc aatggcttcg agcggtcacc tatcgacaga ttgattacgc gaacgctcgg
     1861 cgctacaggg ccttcccctc cgtcgctttc gtacaaccgt ctcactgctc gatgtatcag
     1921 gtcaatcggc agcattccct cgggtatcac ttcctcgatg aacctcccat caaatccacg
     1981 acggggttgc ataataggcc gcacatagac aggtctttcc aagggggctt cgtggtcatc
     2041 taggtcgcca tgacatataa gcgatgccat cgcagtaccg tggacgcgtt cttttgcctg
     2101 ataggcgctt tcaaatccat ccgggtcatc aacgattagt cgcccatcga ggagtcgatg
     2161 cttcgttaga ggcaagccat caaacaaggc aacgattggc tccccacttg gaagcactct
     2221 atcaaccaac tcaccaatgt gttgcgtctc actcatatcc tcggggatgc gaattgcgca
     2281 ttgacccact ggatgcacgt acataatgtc ttcgcattgg aagaggcgga actctgccaa
     2341 tgtgtcgact tgcgaaataa attgagacac ctccttgata gggattcttc ccaatacacc
     2401 atggtatgca atttcaggta taacacactg gctggtgatt tcgccgccaa gagtgtgaac
     2461 taacttttcg agattactct cggttgtctg tcgtcgctca ggggcatttc gataccacaa
     2521 ttctacttca aaaggtacct tttcctgacc gtatccaatt ctttccttcc agtcctccca
     2581 gagacctgta tcttttatgc ggtcatgagc atcccatggt cgaatggtgc gaaggtgata
     2641 gcggaataag ctcttcagcg gtgtaagccc tctgggaaat tttgcttcgg gatcgctttg
     2701 ccatatattg aacagactca gtaactcatt gagg
//
LOCUS       DAOSAI010000329         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_24155_length_2723_cov_4.366192, whole genome shotgun sequence.
ACCESSION   DAOSAI010000329 DAOSAI010000000
VERSION     DAOSAI010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24155_length_2723_cov_4.366192"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1163
                     /gene="trpS"
                     /locus_tag="PLM83_09640"
     CDS             183..1163
                     /gene="trpS"
                     /locus_tag="PLM83_09640"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOK71702.1"
                     /translation="MAKGRILSGNRPTGKLHLGNFAGALANWVKLQDDYECFFMVADW
                     HALTTGYEDTSELKNYTRQMLIDWLSAGLDPERSAIFRQSDVKEHAELHLLLSMITPL
                     GWLERCPTYKEQLQQLSGKSITNYGFLGYPVLMAADILVYKADTVPVGEDQLPHLELA
                     REIARRFNYLYTPVFLEPQAKLSQVPILPGIDGRKMSKSYGNTIALSDPPEKVREKVN
                     AMVTDPQRIRKSDPGHPDVCTVYAYYKGLAPELIDGRRQECEAGKIGCVACKKELANV
                     LLEILGPIHERRHELEAQPGFVESVLEEGAAKARPIAVQTMTEVREAMRI"
     gene            1160..1936
                     /locus_tag="PLM83_09645"
     CDS             1160..1936
                     /locus_tag="PLM83_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOK71703.1"
                     /translation="MKVETPVFSGPLDLMLSLIEKKEIDICEVSISEITDDYLEHVSS
                     MEWLDIDQAAEFLVIAATLLYMKVKALLPEQELAEEFGVEEDELPDPGADLVARLIEY
                     RRYRDAARVMRSLEATASRMYTRPPRPLPKPAMVKVNPLDGISAKDLFDLYVKAATSV
                     PEEFTHLPEDEIDIVPQMASVLTRLARRGRLGFHEFLGEDASRPVIVATLLAMLELVR
                     RGRISATQPMPFGEIWMFPQKEKASSGGVSDESPRSASGN"
     gene            1896..2609
                     /gene="scpB"
                     /locus_tag="PLM83_09650"
     CDS             1896..2609
                     /gene="scpB"
                     /locus_tag="PLM83_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOK71704.1"
                     /translation="MCPTNLQEAQAAIEAILFAASFPLRAEEIASAIGVDESSTRKLL
                     RLIGDRHSSPESGVALVETGGGFIMLSRPEYAPYIERLSERQRPPSLSPASLETLAIV
                     AYRQPVTRGEIERIRGVSPDSAISTLLERGLICEKGRKESIGRPILYGTTPDFLLYLG
                     LSSLDDLPVLPDGEGLPADAPQGADEQGALMSPLPGLGKDGDEAGGNAHWDMPGEIDL
                     ESMFDGMSATDANCDEEDD"
BASE COUNT          614 a          694 c          820 g          595 t
ORIGIN      
        1 gacgggtcaa agatcctcgc agcgctcctc aagggcaagg gcgcacgttt gtactatacg
       61 ttggagaagt actcttacat cgtgcttttg ttgttcatag cgacgccgtt ggcgagctgg
      121 atcttgagcc cgatcatatt gacgctttac agagcgataa tgtagtgtag aaggtggttt
      181 gtttggcaaa agggcgcata ctgagcggga acaggccaac gggaaaactt catctgggca
      241 acttcgccgg agcgctggcc aactgggtca agctccagga cgattacgag tgcttcttca
      301 tggtagccga ctggcacgct ttgaccacgg gatatgagga tacatctgag ctgaagaact
      361 acacccgcca gatgctgatt gattggctgt ctgcgggctt ggatcctgaa agatccgcaa
      421 tcttcaggca gtccgacgtc aaagagcacg cagagcttca tctgcttctc tcaatgatca
      481 cgccattggg gtggctagaa aggtgcccca cttacaagga acaactgcag cagctgtcgg
      541 ggaaaagcat aactaactac ggttttctgg gctatccggt gcttatggcc gctgacatac
      601 tggtgtataa ggccgatacg gtgccggttg gagaggacca gcttccacac ttggagttgg
      661 cacgcgagat agctcggagg ttcaactacc tatacacgcc cgtgtttttg gagcctcagg
      721 ctaagctgag ccaggtaccg atccttccgg gaatcgacgg acggaagatg agcaagagct
      781 acggaaatac aatagccctg tccgatcctc cggaaaaagt gcgcgagaag gtgaatgcga
      841 tggtcaccga tcctcagcgc atacggaagt cggatcctgg ccaccccgat gtctgcaccg
      901 tttatgctta ctataaaggt ctcgcccccg aactcatcga tgggcgcagg caggaatgtg
      961 aggctgggaa gatcggctgc gtcgcctgca aaaaggaact agccaatgtg cttcttgaaa
     1021 tcttaggtcc aatccacgaa aggcgccacg agcttgaagc ccagcccggc ttcgtcgagt
     1081 ctgtcctcga agagggagcg gccaaggcac ggcctatcgc ggttcagacc atgaccgaag
     1141 tgcgggaagc catgaggata tgaaggtaga gacccccgtc ttctcgggcc ccctcgatct
     1201 gatgctctct ctcatcgaaa agaaggagat agacatttgc gaggtatcta tcagcgagat
     1261 taccgatgac tacttggaac atgtatcatc gatggagtgg ctcgacatag accaggctgc
     1321 agaatttttg gtgattgcag ccacgttgct ctacatgaag gtcaaagctt tgcttcccga
     1381 gcaggaactc gcggaagaat tcggcgtgga agaggatgag ttgcctgacc ccggcgcaga
     1441 cctggtggca cgcctcatcg aatataggcg ctatcgcgat gcggcgcgtg tcatgcggag
     1501 ccttgaagcc acggcttcga ggatgtacac gcgcccgccg cgtccgcttc caaagccggc
     1561 tatggtgaaa gtgaacccgc tggatggcat atcagctaag gatcttttcg acttgtatgt
     1621 caaagctgca acgagtgttc ccgaggaatt cacccatctg ccggaggatg agattgacat
     1681 tgtgcctcag atggcgtctg tcctgacgcg tctggcgcgt cgcggacgct tgggctttca
     1741 tgagttcctc ggggaggatg cgtcccgccc tgtcatcgta gcaacgctcc tggccatgct
     1801 ggagctggtt aggcggggaa ggatctctgc cactcagcca atgcctttcg gcgagatatg
     1861 gatgttcccg cagaaggaaa aggcgtcatc cggaggtgtg tccgacgaat ctccaagaag
     1921 cgcaagcggc aattgaagca atactgttcg cggcctcgtt tcccctccgg gctgaagaga
     1981 tcgcatctgc cataggggtt gatgagtctt cgaccagaaa actcctccgg ttgatcgggg
     2041 atagacattc cagcccagaa tcgggtgtag ccttggtgga aactggcggc gggttcatca
     2101 tgttgtcgcg tcccgagtac gctccctaca tagaacggct ttccgaaaga caacgtcctc
     2161 cgtcgctttc tccggcttcc cttgagacac tggccatagt tgcgtatcgg cagcctgtca
     2221 ccaggggaga aatagagaga atcagagggg tatctcccga ttcagccata tccacgttgc
     2281 tcgagcgagg gcttatctgt gaaaagggac gcaaggagag tatagggcgg ccgatccttt
     2341 acggaactac gccggatttc ctgctttact tggggctcag ctcgctcgat gatcttccgg
     2401 tgcttcccga tggtgaaggg cttcctgcag acgcgcctca aggtgccgat gagcagggtg
     2461 cgttgatgtc acctcttcct ggcttgggga aggacggcga tgaggctgga ggcaatgcgc
     2521 attgggatat gccaggtgag atcgatcttg agtcaatgtt cgatggtatg agcgcgacag
     2581 atgccaactg tgatgaagaa gacgactgac cgagtcaggg tcgaccgaag aatctgggcg
     2641 ttccgggcct gatttcacgc gatggtgaat actgctcctg ctgcgcggca gaatacgagc
     2701 gggagtggtg cgggtgagat ggg
//
LOCUS       DAOSAI010000330         2719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_24211_length_2719_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAOSAI010000330 DAOSAI010000000
VERSION     DAOSAI010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24211_length_2719_cov_3.000000"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLM83_09655"
     CDS             <1..640
                     /locus_tag="PLM83_09655"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOK71705.1"
                     /translation="DEGEDGICRSVLDVELYQLKIQIEVEGFDIWRRVLVPSTFSFRH
                     LHNVIQTVFDWQNYHLHMFEARKQGSKVKRIVMDDDPETLGCMDPDSCEILQDRFTAL
                     EEIFPNYDGIMYEYDFGDSWQHKITLEQIVSSDALEATYLDGRGERPPEDVGGAWGYI
                     EYMRIMADETDPEHEDMRLWAASQAERNRTPEQINNRLKHCMKGYTYSRSLL"
     gene            741..851
                     /locus_tag="PLM83_09660"
     CDS             741..851
                     /locus_tag="PLM83_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOK71706.1"
                     /translation="MKKIGRNAPCPCGSGKKYKKCCAQPNVGPIAEILVV"
     gene            865..1671
                     /locus_tag="PLM83_09665"
     CDS             865..1671
                     /locus_tag="PLM83_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK71707.1"
                     /translation="MSTEEIIERLDSLGIPFEKEIFLQDIEEYYSAEQLSENWFRIFD
                     VTAEGRDADFPWLAARVLWDRLAPAYNMSMEHMGDLVHEGNQYSLANDPVRACDTWLE
                     VWEAIKYKCKPKSKDLKELDKEYCGVFFVTNLCQDLELEFHNAGLRDKSYFEKRIDYC
                     RDFLNRFPNEDELITHNMRRAIADSYASLGDYEQADAEFDKLAEDYPNSPWGYIGWGD
                     IYFFEKKVDYDRAKELYMRALAIAKDEYDVMAVNESLEDLIKTEQLEIPS"
     gene            complement(2341..>2719)
                     /locus_tag="PLM83_09670"
     CDS             complement(2341..>2719)
                     /locus_tag="PLM83_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71708.1"
                     /translation="VSIVQENGRGLFFMAYLWGNRYVPTVGINEAGLFASTQLHHNET
                     IVDIDAGDDSLYMYQLFADSLVSLDRVSQVREIAATRRVVDAYPYRPTHNLFADPTGQ
                     AIVVETIATNRHITRPNLESRSV"
BASE COUNT          701 a          659 c          776 g          583 t
ORIGIN      
        1 cgatgaaggt gaggacggaa tctgccgatc agttctagac gtagagctat atcaactgaa
       61 gatacagatt gaagtagaag ggtttgatat atggaggaga gtcctggtgc catcgacctt
      121 ctccttcagg catctgcaca atgtcattca gacggtcttt gactggcaga actatcacct
      181 gcacatgttt gaagccagaa aacaggggtc aaaggtaaag cggatcgtaa tggacgatga
      241 tccggaaacg ttaggatgta tggaccctga ctcctgtgaa atcctgcagg atcggtttac
      301 ggcacttgaa gagattttcc caaactatga tggaatcatg tacgaatacg attttggcga
      361 ttcatggcag cataagataa cacttgagca gatagtgagc tctgacgcac ttgaggcgac
      421 ctacctggac ggaagaggcg aaaggcctcc cgaggatgta ggcggagcct ggggatatat
      481 agagtacatg cggatcatgg cagacgagac ggatcccgag catgaggaca tgagactctg
      541 ggcagcgagc caggcggaga gaaaccggac ccccgaacag ataaacaaca gactcaagca
      601 ttgcatgaag ggatatacgt attcgcgatc gttgttgtaa agatactcct tttgtcgcag
      661 ccttagctcc cctctcgtcg gtgagcagat cgattggcct ggttatgtcc ttaaactcgg
      721 actatgtgga gggatagcca ttgaagaaga taggaagaaa cgccccctgt ccttgcggga
      781 gcggcaagaa atacaagaaa tgttgtgccc agcccaacgt tggtccgatt gcagagattt
      841 tggtcgtatg aggaagtaga agaaatgagt acagaggaga tcatcgagcg gctggacagt
      901 ctcggcatac cattcgaaaa ggagatcttc ctgcaagaca tcgaggaata ctactctgct
      961 gagcaactct cagagaactg gttcagaatc ttcgacgtaa ctgcggaagg ccgggatgca
     1021 gacttcccct ggctggctgc acgggttttg tgggacaggc tggcgccagc ctataatatg
     1081 tcgatggagc acatgggcga cttggtgcat gagggcaatc agtattcatt ggccaatgat
     1141 cccgtaagag cctgcgatac ctggctggag gtttgggaag cgataaagta caaatgcaag
     1201 ccgaagtcca aagacttgaa ggagcttgat aaggagtatt gtggagtatt cttcgtcacc
     1261 aatctatgcc aggacctgga gcttgaattt cataacgccg ggctacgtga caagtcctat
     1321 tttgaaaagc gaatcgatta ttgcagggac tttctaaacc gttttcccaa cgaagacgag
     1381 ttgatcactc acaacatgag acgcgccatt gcagactcat atgcgagctt gggcgattat
     1441 gagcaagctg atgcagagtt tgacaagttg gcggaagatt atccgaatag cccgtgggga
     1501 tatattgggt ggggagatat ctacttcttt gaaaagaaag ttgattacga tagggcaaag
     1561 gagctctata tgagagcttt ggccattgcg aaagacgaat acgatgtcat ggcagtcaat
     1621 gagagcctgg aagacttgat caaaactgaa cagctggaga taccatccta gagctcgtta
     1681 actgcttccg ggagagcctt ctcaggtctg cggccaacga gcgcgcttcg ccggcagccg
     1741 caaggcacgg gggccggagt tctgcacagc ctcgttgtaa tggagcgcca ccccgaggac
     1801 agggtgatcc ctgaagaatg ccgccgggcc cgcctatggc gcaaccggcg actttgttcc
     1861 ctgagcgtct ttgattctcg cactggccat tcctgggcgt cgggcatgtc ccgctacatg
     1921 gcggggaaca accaggcatc atcgtcggag gcgatcaacg tggggcaggc ctggcaatct
     1981 ggggcgggtt gaccgcggac cgacaatccg cccgaagtat cgggcatcaa ccgcctgccg
     2041 atcatgccca tgctgctttg gctgggtcac gaccccgact gcatcctcag aaactacgtc
     2101 agcacgagac gggcagaata aacacgtggc acgacacgac cgatcccgac acggccttgg
     2161 accttcatga agaccgattc ggcccggagt tcggcgagcc ccgcgcccac attttctatt
     2221 gtttgcacag cccgacctaa aggtcgcggc tgatgctgcg ttttacgtag ccttccagaa
     2281 ggaagatcga aggatatacg tggcccacaa tcccccgatg ccgagaagtc agtgctcttg
     2341 tcagacggaa cggctctcaa ggtttgggcg cgtgatatgg cggttagtag cgatcgtctc
     2401 cacaacgatg gcttgcccgg taggatccgc gaaaagattg tgagtaggcc tgtaggggta
     2461 ggcatcgact acccgccgtg tggcggcaat ctcccgcact tgacttaccc gatccaggct
     2521 caccagtgaa tccgcaaaga gctggtacat gtaaagagag tcatcgcccg catcaatatc
     2581 gacgatcgtc tcattgtggt gtagttgagt tgacgcgaac agacctgcct cgttaatccc
     2641 cacggtggga acgtaacggt tgccccaaag ataagccatg aagaagagtc ccctgccatt
     2701 ttcctgaacg attgaaacc
//
LOCUS       DAOSAI010000331         2719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_24214_length_2719_cov_2.785661, whole genome shotgun sequence.
ACCESSION   DAOSAI010000331 DAOSAI010000000
VERSION     DAOSAI010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24214_length_2719_cov_2.785661"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLM83_09675"
     CDS             complement(<1..351)
                     /locus_tag="PLM83_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71709.1"
                     /translation="MGYYDRWGRPRGFGFPLFVAALVGAVIGGLVVGMLLIGRIPQQQ
                     AAPAPQLSTPSDDEILPPSERLSAEAYSIASAAKKVSPAVVKIATRQEELVFSFFFRP
                     IIQEREGLGSGVIIV"
     gene            complement(408..1835)
                     /locus_tag="PLM83_09680"
     CDS             complement(408..1835)
                     /locus_tag="PLM83_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOK71710.1"
                     /translation="MGSEIRFGTDGWRAVMCDAFTFANVRAVIQAVADYVISHDMRDR
                     GVLVGYDARFLSRGFAEVAAEVLAANSIPTLVTNRDTPTPVVAFTVADLGLAGAVMFT
                     ASHNPPEYNGVKWIPHYAGPADPDITNEIEGHIARRANAGSIPRTMPFEEALAAGIVR
                     SIDPSDRYLSRIRSLVALDTIAESGIKIVYDPLFSTGRGYVDRILAEAGCNVETINGC
                     RDPLFGGGSPDPQEESLGDLREKVLSSGADIGLATDGDADRFGAIDSDGTYLGANQII
                     PLLLLHLIRAGRGQGVVVRTIATTHLIDRIAHAHGIDVIETPVGFKYICQVMRERDVI
                     IGGEESGGLSIGGHVPEKDGILANLLLAEFRAVEGRPLKRSLRAVMDEYGASCTRRID
                     MRFSDNRKRDALIRRFVEKPPLEVAGRRVCDISTTDGVRFTLDGGDWFLVRASGTEPV
                     VRLYVESKSDEGISALLGEFNALLG"
     gene            complement(1957..2589)
                     /locus_tag="PLM83_09685"
     CDS             complement(1957..2589)
                     /locus_tag="PLM83_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOK71711.1"
                     /translation="MKKCNTSAGEIRAAYGRIPNALVQAYTGDGKGKTTAALGLAMRA
                     LGHGYKVRMIQFMKGTSFTGELEAAKAFAPNFEIFQFGRDCKYSDKIRSGEAVCEGCG
                     DCFVRMGDPEPCDIEYARQAYELAGATLDAKAADLVILDEIANAVNFGLLSVEDAVSL
                     VERRPDGVELVLTGRDMPKTLIDMADLVTEMKMVKHPYMRGISARHGIEY"
BASE COUNT          591 a          849 c          661 g          618 t
ORIGIN      
        1 aacgattatt actccggaac ctaacccttc cctctcctgt atgatcggcc tgaagaagaa
       61 agaaaagacc aattcttctt ggcgggtggc gatcttcacc acagctgggc tcactttctt
      121 ggcagcagaa gctattgaat atgcttccgc cgacaatcgt tctgacggcg gaagaatctc
      181 atcgtcggac ggagtagaga gctgaggcgc aggcgccgcc tgctgctgag ggatgcggcc
      241 gatcagcaac atccccacaa ctaatccccc gattaccgct ccgactaaag cggccacaaa
      301 tagtggaaat ccaaatccgc gaggccttcc ccatcgatcg taataaccca tgaaatgcca
      361 cccccgtctg tcacatctaa cgctatcagc agaaacagaa agctctgtca acctaatagt
      421 gcgttaaatt cgccgaggag agccgaaata ccttcgtcag actttgattc tacgtatagc
      481 ctcacaacgg gttccgtgcc tgacgccctg acaagaaacc aatcaccgcc atcgagcgtg
      541 aatctgacgc catctgttgt ggagatgtcg cacacccggc gccccgccac ctcaagtggg
      601 ggtttctcca cgaatctcct aatcagagca tcccgtttgc ggttgtcgga aaacctcatg
      661 tcgatccggc gtgtgcaaga cgcgccgtat tcgtccatga ccgcgcggag cgacctcttc
      721 aagggcctgc cttcgaccgc tctgaattcg gcaagaagca ggttcgcaag aataccgtct
      781 ttctcgggaa cgtgtccgcc aatcgagagg cctccgctct cctctccccc gattatcacg
      841 tctctctccc tcatcacctg gcatatgtat ttgaatccta ccggtgtttc gatgacgtcg
      901 attccatgag catgggctat cctgtcgatc aaatgagtgg tggcgatagt acgcacaacc
      961 accccttgcc cccttccagc ccgtatcaga tggagcaaga gcagtgggat tatctgattt
     1021 gcccccaaat atgttccgtc tgaatcgatg gcgccaaatc tgtcggcgtc cccgtccgtg
     1081 gcaaggccaa tatctgcgcc cgaagagagc accttctccc ggagatcacc caagctctcc
     1141 tcttgaggat cgggggatcc tcctccaaag agcggatccc tacagccatt tatagtctca
     1201 acgttacatc ctgcctctgc caatatcctg tctacgtatc ctctgcccgt agaaaaaagc
     1261 gggtcgtaca cgatcttgat gccgctttcg gcaattgtat cgagagccac aagggatcga
     1321 atgcgcgaca ggtatctgtc tgaaggatcg atcgatctga caattcccgc cgcaagggcc
     1381 tcctcgaaag gcatcgtacg cggaatcgac ccggcattcg cccgccgtgc tatgtgtcct
     1441 tctatttcat tggtaatgtc tgggtcggca ggcccggcat aatgcggaat ccacttcacg
     1501 ccgttgtatt caggcggatt atggctggca gtaaacatga ctgcccccgc aagacctagg
     1561 tcggcgacgg tgaatgccac tacaggtgtt ggagtgtcgc ggttggtgac aagggttggg
     1621 attgagttgg ccgctaacac ttctgcggcg acctctgcaa atccgcggga aaggaaacgg
     1681 gcatcatacc ccaccaaaac gccgcggtca cgcatatcat ggctgatcac gtagtccgcc
     1741 accgcctgaa tgacggctcg gacattggcg aaggtgaaag catcgcacat taccgccctc
     1801 cacccatctg ttccaaatct gatctcgctt cccatatgag tccctcctcc tcatgttgca
     1861 gcactattgt ctcagcacaa agaaggaccc tagccgggct ttcgcggaat gtcggccttc
     1921 ggttctctgc ccgcgttcca tgccgccaaa gccgagtcag tactctattc cgtgccttgc
     1981 ggagattccg cgcatgtacg ggtgcttcac catcttcatc tcagtgacca ggtccgccat
     2041 gtcgatgagg gtctttggca tgtctcgccc tgtgaggact agctcgacac catccggacg
     2101 gcgctccaca agggagacgg catcttcgac gctgaggaga ccgaagttga ctgcattggc
     2161 gatttcgtct agtattacca gatcagccgc tttcgcgtca agcgtggcgc cggcaagctc
     2221 gtaggcctgt ctcgcgtatt cgatgtcgca gggttctgga tcgcccatgc ggacgaaaca
     2281 atcaccgcat ccctcgcaaa cagcctcccc tgatctgatc ttgtcagagt atttgcaatc
     2341 cctcccgaac tggaatatct cgaagttggg agcgaatgcc tttgccgcct ccagctcccc
     2401 tgtaaagctc gtccccttca tgaactggat catccgcacc ttgtagccat gtcccaaagc
     2461 ccgcatcgcc aggcccagtg cggccgtggt cttgcctttt ccgtctcccg tatatgcttg
     2521 gaccaaggca ttgggaattc ggccatatgc cgcccttatc tcaccagccg acgtattgca
     2581 ttttttcacc cattgacgcc tcctcagttc acaagtcgca tctatcatcc cttgccgtct
     2641 ccatcagagc ttcccgatat cacgaaaacg gcaggcctat ccaattctgg acggcgggcc
     2701 acaaccgccc tcacgccat
//
LOCUS       DAOSAI010000332         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_24594_length_2690_cov_4.818975, whole genome shotgun sequence.
ACCESSION   DAOSAI010000332 DAOSAI010000000
VERSION     DAOSAI010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24594_length_2690_cov_4.818975"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLM83_09690"
     CDS             complement(<1..393)
                     /locus_tag="PLM83_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HOK71712.1"
                     /translation="MKKAIVLTDGEHYPAVTHDAIEVLKGELDILAAVFIGGTEKIGS
                     DSDLARLGVPVVHEDDYLHAIAAAIDKYDPDEVVDLSDEPVVGYKERFEIASNVLAKG
                     VAYRGADFQFTPPVEAARVSRPSISVIGT"
     gene            complement(415..1197)
                     /locus_tag="PLM83_09695"
     CDS             complement(415..1197)
                     /locus_tag="PLM83_09695"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK71713.1"
                     /translation="MLKKDSFIPLYYQLKEELKEKIVSGEWEPETRIPSIRELCETYD
                     ISTTTAKQAISELVHEGLLYSVQGKGTFVSVPQWPMEYKETTYITDEMRLASRIRALG
                     HEFWAEVLTIDRVAASNLVSAYLAIEDDSEVLRIRRLKKVDGIPMLIETTFISPDVAI
                     GIEEADLSRSLFRILRDEYGIVLKRSDETFRPVFLGSLEADLLGQSVGALAMLNERIS
                     YAESPTPILYAKSLIRGDMCKTYVDLTNMRKVKEMVVNASSF"
     gene            1765..2136
                     /locus_tag="PLM83_09700"
     CDS             1765..2136
                     /locus_tag="PLM83_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71714.1"
                     /translation="MEIREHAIIPLGYGKYVRSDKIIALDPIEDERGPGRRTRVYVDQ
                     LPGPLIASRTENSILASIVRTPTEVLEATAALELLQDLLDDIEQIGPMLRKSIKEEAG
                     LDLEELERKIREILTHELNAE"
     gene            complement(2457..2542)
                     /locus_tag="PLM83_09705"
     tRNA            complement(2457..2542)
                     /locus_tag="PLM83_09705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2507..2509),aa:Leu,seq:taa)
     gene            complement(2507..>2690)
                     /locus_tag="PLM83_09710"
     CDS             complement(2507..>2690)
                     /locus_tag="PLM83_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71715.1"
                     /translation="VVKSELGAISVFDGSAGGGFGNPLQATCVAQGARSWYTFLEVIC
                     DNKVEAGVAERQTQGT"
BASE COUNT          697 a          721 c          627 g          645 t
ORIGIN      
        1 ggttcctatg accgagatac tcggcctgct tacgcgcgcc gcctctacag gaggagtgaa
       61 ctggaaatcc gcgccccggt aagcgacacc ctttgcaagc acattgctgg caatctcaaa
      121 ccgctccttg tagccaacta caggctcgtc gctaagatca acaacctcat ccggatcgta
      181 tttgtctata gcggctgcga tagcatggag atagtcatcc tcatgcacta caggaactcc
      241 aagtctggca agatcagaat cagaaccaat cttctcagtg ccgccaatga aaacggcagc
      301 caatatgtcg agctccccct tgagcacctc tatggcatca tgagttaccg cagggtaatg
      361 ctcaccgtct gtaagtacaa tagctttctt cataatgctc caccaccgtt gttgctagaa
      421 tgaagatgca ttgacaacca tctcctttac cttgcgcatg ttggtgagat ctacatatgt
      481 cttgcacata tccccacgta tcagagactt tgcatataga attggtgtgg gcgattccgc
      541 gtaagaaatg cgctcattga gcattgccaa tgcgccaact gattgtccaa gaaggtcagc
      601 ttctaggcta ccgaggaaga ctggccggaa ggtctcgtcg ctccttttca ggacaatacc
      661 gtattcatca cggagtatcc gaaatagcga tcttgataga tcggcctctt caatgccgat
      721 agccacatcg ggggagatga aagtcgtctc aattagcatt ggaatgccat cgactttttt
      781 caagcgtctg atccttaaca cttccgagtc gtcttcaatg gccaaatacg cactgacaag
      841 attggaagcc gcaacccggt ctatagtaag aacctccgcc cagaattcgt gtccaagagc
      901 tcgaattctg gacgcaagac gcatttcgtc ggtgatatag gtcgtctcct tgtactccat
      961 gggccattgc ggaactgaaa caaaagttcc cttcccttgt acagaataga gcaggccctc
     1021 atgaactaat tccgaaatgg cctgcttagc tgttgtagtg cttatgtcat aggtttcgca
     1081 caactcacgt attgagggaa tgcgcgtctc gggctcccat tcgcccgaga cgatcttctc
     1141 cttgagctct tctttaagct gataatacag gggaataaaa ctgtccttct taagcattgg
     1201 cacgaaagcc tccataccgt aatggacctt gtggcatatt gccagctaaa ccaagtgtgt
     1261 gtgttaacgt gttatcgctt actgtatttc tacatttcct aagcgattcc tcttttaaga
     1321 ccttccattt atacatccat taattacaac agccatctgc tgccacgcca tgcccatttc
     1381 attcacaaac ctctcgagca gcccatgatc tgcagttaca gcagctgcat aaccatgttg
     1441 agccgccact aggttcacaa ggatgaagtg cccgctataa ccgctggcgt tcgcggcagc
     1501 caaggttcat ttaccgagga gatgtctaag gatataggcg atgtggttag aagccgtaga
     1561 aggcatcatg gatagagtaa gaacccttga atgggtccgg cagatactat tcgtcaccaa
     1621 gttgcccatc gaaggcgaac agtcaaaatg tgcgtaggct aaccgatacg ggatggttct
     1681 gtgcagagca cgatggagtg atgggactgt tctttgctgg aaaacctaat tgaagtagat
     1741 cagaactaaa ggacgtgaac tccgatggaa atcagggagc atgccatcat acctctcggc
     1801 tatggcaagt atgtgcgttc agacaagatc atcgccctcg atcccataga agatgagcgg
     1861 ggacccggtc ggcgtaccag ggtctacgtt gaccagctac ccggcccatt aatcgcttca
     1921 aggaccgaga actccatcct agcaagcatc gtcaggaccc caacagaggt tcttgaagcc
     1981 acagccgccc ttgaactcct tcaagatctt ctagacgaca tagaacagat cggtccaatg
     2041 ctgcggaaga gcattaagga agaggccgga ctcgacttgg aggaactcga gcgcaagata
     2101 agggagatat taacccatga actgaatgcg gaatgagctt gtcacacaac tgctttccgc
     2161 taccggatgc gccgtaaaac ccacgactcg gggtcacgtt ccgatatcat attgacccga
     2221 gagatgacgt tatggttgtt tttcggcatg tgtacgtatt taagaccccg tcttagattc
     2281 attggcaagc tgattttccg ctggcaacgc agtttgctcg gtctgtgatc tgcttctgcg
     2341 ctgtctctga atttccatca cacgatggct attcgaaagc gcctaaacac atcgacaata
     2401 agacccgctg gggctgaaaa ccaaaacagc ggggcctact ccccgctaca aatagctggt
     2461 gccggaggcg ggattcgaac ccgcacgtcc ttgcggacag aggattttaa gtcccctgcg
     2521 tctgcctttc cgccactccg gcctcgactt tattgtcgca gataacctca agaaaagtat
     2581 accacgaccg cgccccttgc gctacgcacg tagcctgcaa aggattacca aagccgcctc
     2641 ctgcagaccc atcgaacaca gaaatagcgc caagctccga tttcacaact
//
LOCUS       DAOSAI010000333         2679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_24724_length_2679_cov_3.977896, whole genome shotgun sequence.
ACCESSION   DAOSAI010000333 DAOSAI010000000
VERSION     DAOSAI010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24724_length_2679_cov_3.977896"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(519..1094)
                     /locus_tag="PLM83_09715"
     CDS             complement(519..1094)
                     /locus_tag="PLM83_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71716.1"
                     /translation="MQIYLLRNEGFSVGQASAVVAVKTFCNALAKVIVAFTASSWTVL
                     SGIGWRVPRQTKVVLLTASLLYVAFSIFSLFLMNDPRRIETVIAPILRSRFAAKLLRH
                     GRGERVLRAIRKWIAEFQIAFAHYWERERSTLLGVIYLSVAGWLAIVLVPTMIPGGAR
                     GEGAQRTGDGQRDSVSYGFVVCSHSWGEWRG"
     gene            complement(1069..1389)
                     /locus_tag="PLM83_09720"
     CDS             complement(1069..1389)
                     /locus_tag="PLM83_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71717.1"
                     /translation="MDERLVRGLVIAFIFGTVALLVVFKISGVNVTWSDLTRIDLVVV
                     IVALALVTATWVFDSLRMSALVRAIGDTPSFWSRIRVARCFLGQLDAVQRGRRTYADL
                     SAEE"
     gene            1731..2087
                     /locus_tag="PLM83_09725"
     CDS             1731..2087
                     /locus_tag="PLM83_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOK71718.1"
                     /translation="MNTDTEAIRPSFTLSPRFQGIVHEIEATLRGIYGDRFLGLVLFG
                     SHARGTSVQGSDIDLVLLLQDCSGLSERQHYSEAIADLSLRYDTVISLIPMGIDEYRS
                     GKTPFLLNVRKEGVRL"
     gene            2084..2470
                     /locus_tag="PLM83_09730"
     CDS             2084..2470
                     /locus_tag="PLM83_09730"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOK71719.1"
                     /translation="MSSPDVTDLLAKADRSLAAAEHLFTHGFPEFAVGRAYYAMFYSA
                     EALLLNRDLSFSKHSAVIAAFGQHFVKTGILDASLHRHFLEAFDLRNLADYGAVRTIE
                     DADVHSVLENAKAFCIAIKNHIASLE"
     gene            complement(2483..>2679)
                     /locus_tag="PLM83_09735"
     CDS             complement(2483..>2679)
                     /locus_tag="PLM83_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71720.1"
                     /translation="VSKFRGVDCLRARDASQALGRDCSHAGERRRKRCRMKGLIKYEV
                     GVLRYFNETKQRRFASGHTC"
BASE COUNT          665 a          823 c          616 g          575 t
ORIGIN      
        1 cgatttacct gggcttttga gcctggttcc gcgaacctct ctgaaaagct agtcctctta
       61 ggcttgtgtc cgagtaataa acactgttac cccagctaga tggcttgcgc gaacctcccg
      121 gggaaatcgg catcacttga ggtccgcggt aggactttgc gtatacagca ctccttccaa
      181 attgcaagca acatggtgcc tacacaacag caaaaccaaa gcagcattac ctaagcattt
      241 gacataaatg tggctccact gccttaaata taatacatac tacgttacat atccatttcc
      301 tctacggatc tacgaaatcg tcccccaatc ataatacctc cacgagtatc atctgctaca
      361 cccccgccca gaattcatcc ctcatcccct gccgacccaa ggtcaaagtg ccccccacaa
      421 gcagagtgaa atagtaggta cgcagccgcc agcgggcgac agccaagcct atcagggcca
      481 gtgggacgac catcctgaac aattcagcaa accccagctc agccgcgcca ctcgccccag
      541 gagtgggaac atacgacgaa accatatgaa acagaatcac gttgcccatc acctgttcgc
      601 tgagcgcctt cacccctagc cccgccaggg atcatagtgg gtaccaaaac aatagcaagc
      661 catccagcca cgctcaaata gataaccccc agtagggttg agcgttcacg ctcccagtag
      721 tgcgcaaacg cgatctgaaa ctcagcgatc cacttcctta tcgctctgag aacgcgctca
      781 cctcttccat gtcgaagcag tttggcagca aatcggctgc gaagaattgg agcaataacg
      841 gtctcgatcc gccttggatc attcatgagg aagagcgaaa agatagagaa tgccacgtac
      901 agcaaggacg cggtcaagag caccaccttc gtctgacgcg gcacccgcca gcctatgccc
      961 gacaagacag tccatgaaga agcagtaaat gccacaataa cctttgcaag agcattgcag
     1021 aaagtcttca ccgctactac tgccgatgcc tggcccacgc tgaagccctc attcctcagc
     1081 agatagatct gcataggttc tccgcccgcg ttgaacggca tcaagctggc caagaaaaca
     1141 ccgggcgaca cggatcctgc tccaaaagct cggcgtgtca cctatggccc gcaccagggc
     1201 cgacatgcgg aggctatcga aaacccaagt cgccgtcacc agcgcaagcg ccacgatgac
     1261 cacaaccagg tcgattctcg tcaaatccga ccaagtgacg tttactcctg atatcttgaa
     1321 caccacgagg agagccactg tcccgaagat gaaagcaatg acgagtccgc gcaccaaccg
     1381 ctcatccatc ttgctgccaa tgcctcccgc ccgcactaca ccgtgggtca acagcgccag
     1441 cctaggtgca agccgactcc gcaaactgtc ctgtccttga tatcatcgat aatctgtccc
     1501 atcgaggcct ctcgtaaaga caggagccgg tcagcaaatt gacagaccac gcaagtatac
     1561 gactggattt gggcccatat tcagaaatcc ctccagcttc caacctccca cagtactcca
     1621 catgacaaga cgtgacccga tgctgtaccc aggtgcgaca gataggtgga accatttatg
     1681 tctatgagtc tggtataatg gtgccgacaa cacatgtagg agcggtccag atgaacactg
     1741 atactgaggc tattcggccg tcattcactc tctcacctcg cttccaaggt atcgtgcacg
     1801 aaattgaagc cacgctgcgt gggatctatg gcgaccgttt cctgggtctt gtgcttttcg
     1861 gttctcatgc ccgaggcaca tcggtccagg gttcggacat cgatctagtg cttctacttc
     1921 aggactgtag cggtcttagt gagagacaac actattctga ggccatcgct gacctgtccc
     1981 tccgatacga cacggtaatc tccttgatcc ccatgggaat cgacgagtac cgttcgggca
     2041 agacgccgtt tctgctcaat gtccgcaaag agggagtgag gctgtgagct caccggatgt
     2101 aacagatctg ctcgcgaagg cggatcgcag ccttgccgca gcggagcacc tattcacgca
     2161 tgggtttccc gaattcgccg ttggccgggc atactatgcc atgttctact cggcagaagc
     2221 acttctgctc aaccgagatc tttcgttttc caagcactcc gccgtaattg cagctttcgg
     2281 gcagcacttt gttaagaccg ggatcctaga tgcctccctt caccggcact tccttgaggc
     2341 ctttgatctt cgcaatctcg ccgactacgg tgcggtccgc actatcgagg atgcagacgt
     2401 ccactcagtg ttggagaacg ccaaggcttt ctgcatcgct atcaagaatc acatagcttc
     2461 gctagagtaa tgaatcgcca gctcaacaag tgtgtccact ggcaaacctt cgttgcttcg
     2521 tttcattgaa gtaacgcaag acgccgacct cgtatttgat gaggcccttc atcctacagc
     2581 gctttcttcg cctctcacct gcgtggctgc agtctcttcc caaagcttgc gaggcgtctc
     2641 tagctctaag gcaatctacc ccgcgaaact ttgatactc
//
LOCUS       DAOSAI010000334         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_24852_length_2670_cov_3.279159, whole genome shotgun sequence.
ACCESSION   DAOSAI010000334 DAOSAI010000000
VERSION     DAOSAI010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24852_length_2670_cov_3.279159"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLM83_09740"
     CDS             complement(<1..510)
                     /locus_tag="PLM83_09740"
                     /inference="COORDINATES: protein motif:HMM:NF025057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOK71721.1"
                     /translation="MRRRKRNDESGGSQPSWMTTYSDLMSLLLTFFIMLFAFSSIDMQ
                     RFREALISLQGALGVLSGGPALLEPGEMPIPPDDPSQIGDDTMSQARTDLVIIKGQLE
                     QYLEEKGLRGKVHVEMNKRGLLIRFTDTVLFDLGMATLRPDSRMLLRDLAKFIGPIQN
                     EVVVEGHTDN"
     gene            complement(507..1352)
                     /locus_tag="PLM83_09745"
     CDS             complement(507..1352)
                     /locus_tag="PLM83_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOK71722.1"
                     /translation="MDSATVIGFILGVACILIALMQSDAGLRPFWDIQSVLITVGGAL
                     GAVIISFSAEQLKDIPKMIKIAFRSEEEDPLVIIDGMVDLAEMARREGLLALEDSLNR
                     TDDPFLQRGIQLIVDGTDPELVRDILETDLAYIEDRHRNGKAIFDAMTAYAPAFGMIG
                     TLIGLVLMLRTLDKPSTIGPAMAVALITTLYGAVIGNLVTGPIATKLGVKSAEEIRLR
                     EMMLEGILSIQSGENPRIVEEKLMGFLAPKDRLRAQERAGMRRGGARSRREMAEDTGG
                     SGTTL"
     gene            complement(1357..1581)
                     /locus_tag="PLM83_09750"
     CDS             complement(1357..1581)
                     /locus_tag="PLM83_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOK71723.1"
                     /translation="MIKVTKLNGSPIHINAELIETIAATPDTVITLTNGTKLIVEESV
                     EKVVDMIIRYHRLINRRAVIVRDSAEKQTR"
     gene            complement(1714..>2670)
                     /locus_tag="PLM83_09755"
     CDS             complement(1714..>2670)
                     /locus_tag="PLM83_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOK71724.1"
                     /translation="VVRDSSGGLMFTRDGAFKIDAEGNLTHPSTGFMVQGWMADPKGT
                     IDRSGEIQGLKIPLGTSMSAKATDRITFVGNLNAEGDVGTLHSTSVPIYDSLGIQHTL
                     ILEFEKTGDKAWTWTARYGELDSDTGEIPSVGTGVVTFDENGAVVRGQDPSQDPAAPT
                     ITFTPAGGAADLKVNLDFTALSQFGEACSVNWSTQDGYAAGTMETFNIDDRGIITGVY
                     SNGQYKIIGQMVLASFANAEGLTRQANNMFAATTNSGSPQLGEATTGGRGSVMGTTLE
                     MSNVDLAREFTDMIITQRGFQANSRVITAADEMLQELLALKR"
BASE COUNT          568 a          822 c          622 g          658 t
ORIGIN      
        1 attatcggtg tgcccttcaa ctacgacctc attctgtata ggcccgatga acttcgccag
       61 atcgcgcaga agcatcctgg agtcgggcct cagtgtcgcc atgcccaagt cgaacaggac
      121 cgtgtctgtg aatctgatca gaagccccct cttgttcatc tcaacgtgga ccttgccgcg
      181 aagccccttt tcttcgagat actgttccaa ctggcccttg atgatcacca gatccgtgcg
      241 ggcctgcgac atggtatcgt cacctatctg cgacgggtca tccggaggaa tgggcatctc
      301 tcccggctcc aataaagccg ggcccccgct caatacgccc aacgcgccct ggagagaaat
      361 caacgcctct ctgaaccttt gcatgtcgat cgaagagaaa gcaaagagca tgatgaagaa
      421 ggtcagaagg agcgacatca ggtcgctgta ggtcgtcatc caagaaggct gcgaccctcc
      481 cgactcatca tttctcttgc gcctcctcat agcgttgtgc cactccctcc tgtgtcttcc
      541 gccatctccc gccgggacct ggcgcctccg cgcctcatcc cagcccgttc ttgagctcgc
      601 agtctatcct tgggtgccaa aaaccccatg agcttctctt ctacgatacg cgggttctcg
      661 cccgactgta tcgagaggat tccttcgagc atcatctcac gcaagcgtat ctcctcggcg
      721 cttttcacac caagcttagt agcgatagga cctgtgacca agttgcctat cacagcgccg
      781 tacaaagtgg tgataagagc caccgccatg gcggggccta tggtgctggg cttatccaga
      841 gtgcgaagca taagaacgag gccgatcaat gtcccgatca tcccgaaggc cggagcatat
      901 gccgtcatgg cgtcgaatat ggctttgccg tttctatgcc tgtcctctat ataggcaagg
      961 tcagtctcta gaatatctcg caccagttcc ggatctgtgc cgtcgacaat tagctgaatt
     1021 cctctttgca aaaacgggtc gtccgtcctg ttcaggctgt cttcaagggc caggagcccc
     1081 tctcttcgcg ccatttccgc cagatcaacc attccatcga tgatgaccaa cggatcctcc
     1141 tcttcagacc tgaaagcgat ctttatcatc ttggggatat ctttgagctg ctccgccgaa
     1201 aagctgatta tcacagcacc cagcgctcct cccacagtta tcaggaccga ttgaatatcc
     1261 caaaacggcc tgagcccagc atcgctttgc atgagcgcta tgagaataca cgctactccc
     1321 aatataaacc ctataacagt cgcactatcc atttgcctac ctcgtctgct tctccgctga
     1381 atccctgaca atcactgccc gacggttaat aaggcggtga tagcggataa tcatgtcaac
     1441 cactttctcc actgattctt ccactatgag tttggtgcca ttagtgagtg taatcacagt
     1501 atcgggggta gcggctatcg tctcgatcaa ctccgcatta atatgtatcg gcgatccgtt
     1561 caatttggtt acctttatca ttcaacaagg cccccttgta cactactcgc atcagcagag
     1621 gcacaggggg gagagggcta gactctgccc gatccccctc tccccgcctc aatgcttctt
     1681 gccggcacgt gtaacgtcac aacctgccaa cgattatctc ttcagtgcca aaagctcctg
     1741 aagcatctcg tctgcggcag ttatcaccct cgaattagcc tggaaaccgc gctgggttat
     1801 gatcatatcc gtgaactccc ttgcaagatc gacgttggac atctcaagag tcgtccccat
     1861 tacagacccg cgcccgcctg ttgtggcctc gccaagctgc gggctgccgg agttcgtcgt
     1921 agccgcaaac atgttgtttg cctgtcttgt caggccctcc gcgtttgcaa aggaggccag
     1981 caccatctgg cctatgatct tgtactgtcc gttggaatat actcctgtga ttatcccccg
     2041 gtcatcgata ttgaacgtct ccatggtgcc tgcggcatag ccatcctgag tgctccagtt
     2101 aacagaacac gcttccccaa actggctcaa cgccgtaaaa tccaggttca ctttgaggtc
     2161 tgctgcaccc cccgcaggag tgaacgttat cgttggggct gctgggtctt gacttggatc
     2221 ttggcccctc accacggcgc cgttttcatc aaaagtcacc acgcccgttc ctacacttgg
     2281 aatctcgcct gtgtcggaat cgagctctcc atatctggca gtccacgtcc acgctttgtc
     2341 cccggtcttc tcaaactcca atatgagcgt gtgctgaatg ccaagtgagt catagatagg
     2401 gacagaagtg ctgtgcaagg ttccaacatc gccttcggca ttgaggtttc cgacaaacgt
     2461 gattctgtcc gtagctttgg cactcatgct ggttcccagc ggaattttga gtccctgtat
     2521 ctcaccgctc cggtcgattg tccctttggg atcggccatc catccctgca ccatgaaacc
     2581 agtggacgga tgggtgaggt ttccttcggc atctatcttg aacgctccat ccctcgtaaa
     2641 catcaagcct ccactggaat cgcggaccac
//
LOCUS       DAOSAI010000335         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_24881_length_2668_cov_3.197091, whole genome shotgun sequence.
ACCESSION   DAOSAI010000335 DAOSAI010000000
VERSION     DAOSAI010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24881_length_2668_cov_3.197091"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLM83_09760"
     CDS             <1..202
                     /locus_tag="PLM83_09760"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOK71725.1"
                     /translation="VANILAGVVARLASDVPRLLYPKGKFISSGFVAKQEESVRRALE
                     SVGHSVIDRLQFEDWVTLVSEG"
     gene            204..956
                     /locus_tag="PLM83_09765"
     CDS             204..956
                     /locus_tag="PLM83_09765"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOK71726.1"
                     /translation="MRRFFVGCDAISGDLVTISGRDARHIVKALRLKLGDAIVVVDGV
                     GRRYVARITATRETTVEARIESMCDSGVSEPHVPITLVQGLPKADKMDGIIQKCTEIG
                     IARIIPVMSERSVLRPDGASAERRMARWRRIAEEAAKQSGREAIPLVADIGTLKSAIE
                     MLRHEGALMVFPWELEQEYSLHQALSELNPSSFPSVGFFIGPEGGFSNAEVEYARAHG
                     ATTVTLGQRILRTETAGPVVAAIILYHMGELG"
     gene            1146..1493
                     /gene="trxA"
                     /locus_tag="PLM83_09770"
     CDS             1146..1493
                     /gene="trxA"
                     /locus_tag="PLM83_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOK71727.1"
                     /translation="MANSVTPIEITDASFGAEIGDFSGVAIVDFWAAWCGPCRMQGPI
                     IDKLAEEYHAKDEIVKVAKLNVDENPRTAEKYGIMSIPTVIIFRNGKPVDKVVGVTPL
                     AVLKRRVEQVASV"
     gene            2180..2641
                     /locus_tag="PLM83_09775"
     CDS             2180..2641
                     /locus_tag="PLM83_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71728.1"
                     /translation="MKRAHVLRGIAIAAVLVMASMTAGCLKTEIAMSISPGELVCVRD
                     AKFDGTITVEMKGFLAGGKYDTLTVKFFEGGNDEPLATMRVDDLMIVVSPLGKIVKQE
                     LAELDGLVAPAVLWDDEGVFRGAKAQFTLTSTGLGVQLNPLVCEVGLVEAR"
BASE COUNT          628 a          587 c          815 g          638 t
ORIGIN      
        1 agtcgccaac attcttgcag gcgtggtcgc gcgccttgcg tccgacgttc cacgcctcct
       61 gtatccgaag ggcaagttca tatcatcggg gttcgtggca aaacaggagg aatcggtgcg
      121 gcgggctctg gagtctgttg gccacagcgt catcgatcgc ctgcagttcg aagattgggt
      181 gacgttggtt tcggagggtt gaaatgcgca ggttctttgt tggatgcgat gctatatcag
      241 gcgatctggt gacaatatca ggaagagacg cgcgccatat cgtgaaggca cttcgtctga
      301 agcttggcga tgccattgtc gtagtcgatg gagtgggccg ccgatatgtg gcgcggatta
      361 cggcaacccg cgagacgact gtcgaagcca gaatagagag catgtgtgat tcgggtgttt
      421 cggaacctca cgtgcccatc acgttggtcc agggattacc gaaggctgac aagatggacg
      481 gaatcataca aaagtgcacg gagataggga ttgcaaggat cattccagtc atgtctgaac
      541 ggagtgtgct gaggcctgac ggagcttctg ccgagcgaag gatggcgcga tggaggcgaa
      601 ttgcggagga ggcggcgaag cagagcgggc gcgaagcgat tccactagtt gctgacatag
      661 gcactctcaa gtcagcgata gagatgttgc gccatgaagg tgctcttatg gtgtttccat
      721 gggagctcga acaggaatac agtctgcatc aggccctctc cgagctcaat ccttcgtcat
      781 ttcctagtgt gggctttttc attggcccag aaggaggttt ctccaatgcc gaagttgaat
      841 acgcccgcgc ccatggcgcg acaactgtta ccttggggca gaggatcctg cgcacggaga
      901 cagcaggtcc ggtagtcgct gcaattatcc tctatcacat gggagaactt gggtagaata
      961 agccttggaa gatgcgaaaa atgccgtgct gctttaggtt tgcagaatct gttgacacct
     1021 ttcgaccatg gatctataat tcaagtgagg atacccccat accgtatacg gctgccgcta
     1081 atgatgcaga gatcgagatc gcgcgggcaa tgtggggtgg atcttgaggg aggactgact
     1141 gtgtgatggc aaatagtgtc acgccaattg agattacgga cgcaagtttc ggcgcggaaa
     1201 taggcgattt ctcgggggtc gctatagtag atttttgggc tgcctggtgc ggaccttgca
     1261 gaatgcaggg ccctattatc gataagctgg cggaagaata ccacgccaag gacgaaatcg
     1321 tcaaagttgc caagctcaac gtggatgaga atcctcggac agccgagaaa tacggtatta
     1381 tgagcatacc gacggtcatc attttcagaa atgggaagcc agtggataaa gtagtgggag
     1441 tcacgccgtt ggctgttttg aaacgtcgag tggagcaagt cgcctcggtc tgagtcgagt
     1501 cggatttgct caagcgcagg aaggctggct cttgctctat cctgtttact ctggcgattt
     1561 ggtcagggta ttaagacttc tcagtgccct gttatgatgc cagctgcggg aacgcggcat
     1621 cgaaatgggg cattcttttt tggtaaggtg ccgtgcttag tccagtttcg caagtctctg
     1681 ctccaaacgc ccccggttgc attaacagcc aatgtcgagg gaggttctta ttctggacga
     1741 taccatcagt cggtcgtagt tgaacgcttg ggacatggct ggtgctgtaa ggaaccgctc
     1801 tgaagataga ccggacggaa ctgacattgc gaggcggcaa ttcccgtaga atcactgctt
     1861 ccccttggta aacccggcaa atcacctcac aggtgtgtat atggtgaaac atcgctcgca
     1921 acaaacttgg ctcgatacgc ttcccaactg cttttttggg gggccgagca ggttcttaga
     1981 agaaacgacg aaatggcaag gcctacatgc tcagaatgag cttgagggaa tatcgtggga
     2041 tgtcttgaaa attgcgccgt aattgcttac acgatgagtg aggcccatcc gggacaggta
     2101 gcaggaatat aggtcttcta gtagaattat cctaccgcaa tgctgtggtt ccaattgcgt
     2161 catgcgggag ggtctattga tgaaaagggc tcatgttttg aggggtattg ccatcgcagc
     2221 agttcttgtc atggcctcta tgacggcagg atgtctcaag acagagattg ctatgagcat
     2281 ttctcccgga gagcttgttt gcgtgaggga cgccaagttt gacggtacaa tcactgtgga
     2341 gatgaaaggg tttctagcag gcggaaaata tgacaccctg acagtgaagt ttttcgaagg
     2401 cggcaacgat gagccgctgg caacgatgag agttgatgat cttatgatcg ttgtatcgcc
     2461 tctcggcaag atagtcaagc aggaactggc agaactggat ggtctagtag cgcccgctgt
     2521 gttatgggat gacgagggcg tgttcagagg tgccaaagcg caattcactt tgaccagcac
     2581 agggttgggc gttcagctca atccgcttgt atgcgaagtt ggattggttg aggcgagata
     2641 ggatagagca atgtgacgtg atcggatg
//
LOCUS       DAOSAI010000336         2665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_24919_length_2665_cov_3.759770, whole genome shotgun sequence.
ACCESSION   DAOSAI010000336 DAOSAI010000000
VERSION     DAOSAI010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24919_length_2665_cov_3.759770"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLM83_09780"
     CDS             <1..334
                     /locus_tag="PLM83_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK71729.1"
                     /translation="GPRYWVKIGLLAVTIAASILIKRPFCRFACPLGAIWSPFNKASL
                     MRLEVDEGACTQCGACKNVCPVELEVYRDPNSDACIRCLECTRCDAVSVSWSRKSTSA
                     GKACVGGL"
     gene            321..593
                     /locus_tag="PLM83_09785"
     CDS             321..593
                     /locus_tag="PLM83_09785"
                     /inference="COORDINATES: protein motif:HMM:NF022641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HOK71730.1"
                     /translation="MAVCRGQVEEIPHAVDGVSRSGAFYTFISFEQNNAVYRTDHRAL
                     SKPDADEYFGLFQDAGWEHVASYSNTHYFRCLPEACEFPEIHSDRD"
     gene            740..1060
                     /locus_tag="PLM83_09790"
     CDS             740..1060
                     /locus_tag="PLM83_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOK71731.1"
                     /translation="MRPLDRHGRILDVHADEVGLGDLGSLASRAILMIIFLFGSYAWG
                     VPCTESDLDTYVVLPENADMREIDAVKLIRKAIHDKKTMPADVIVSKENESNRWKIAP
                     TIER"
     gene            1990..2631
                     /locus_tag="PLM83_09795"
     CDS             1990..2631
                     /locus_tag="PLM83_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOK71732.1"
                     /translation="MAKGLKDPVSGLTHLAGAIMSVIGLVVLLRAGIANEDLWQIVSF
                     SIFGASLILLYSASAAYHLLNVPSSVELVLRKLDHIMIFVLIAGTYTPVCLGPLRGAT
                     GYVILTIIWALAIAGVFFKVFYLNAPRWLYTGIYLAMGWLIIFATGPLVRAVGLHILS
                     WLLIGGVFYSIGAVIYGLKKPDPFPGRFGFHEIFHIFILLGSLSHFRFIHLLA"
BASE COUNT          614 a          607 c          714 g          730 t
ORIGIN      
        1 cggaccgcgc tactgggtga agatcggcct tttggctgtg acgatcgcgg cttcgatatt
       61 gatcaaaagg ccgttctgcc gatttgcatg tccgttgggg gccatctggt cgccatttaa
      121 caaggcgagc ttgatgcgct tggaggtgga cgagggagct tgcactcagt gtggagcatg
      181 caagaacgtt tgccctgttg aacttgaggt ttaccgagat ccgaattcgg acgcgtgcat
      241 caggtgcctg gagtgcacaa ggtgtgatgc ggtgagcgtg agctggagcc ggaagtctac
      301 cagcgcaggc aaagcttgcg ttggcggtct gtaggggtca agtggaggag atcccgcacg
      361 cagtggatgg cgtttcacgg agcggggctt tttacacatt catcagcttt gagcaaaaca
      421 atgcagtcta tagaaccgat catagagcgc tatcgaagcc cgacgcagac gagtatttcg
      481 gtctcttcca ggatgcaggc tgggagcacg tggcatccta ttcgaatacc cactatttca
      541 ggtgtctgcc tgaggcatgc gagttcccgg aaatccattc tgatagggat tagttgaggg
      601 acgagtatcg gcgcagggac tggataatgc tggcctttat tgtgatatac ttgcctgatt
      661 ggatcaccat tgtcttaaga tcggtcccat cagggaaagg cccatttctg accgggctct
      721 attcgttagg aagacggggt tgcgtcctct tgatcgccat ggccgtatac tggatgtaca
      781 tgctgatgag gtcggactag gcgacctagg ttcgttagcg tctcgagcaa tcctgatgat
      841 catattcctg tttggctcct atgcatgggg tgtcccgtgc acggaatcag acttggacac
      901 ctatgtggtg ctgcctgaga acgcagacat gagagagatt gatgcggtga agttgattcg
      961 caaagccatt catgacaaaa agacaatgcc tgcggatgtg atagtaagca aagagaacga
     1021 gtctaaccgc tggaaaattg cccccactat tgaacggtag tttgtgtagg agagaatagt
     1081 gctgtatgga tgacagcata gtctagggac tgataagtca gtcagtcgaa gaaagcagat
     1141 cagagcttgt tggcgatacc aggttatgga acgctgcgaa taattcgcag tgttttggca
     1201 tgacactacg aatatttcgc ggatagcatt acagttagag acagttaatg gaatgtaata
     1261 ccttttgggg cgccgaatcc ccatatttat ccacataaca ggccagcgca atagcatgaa
     1321 taggtaatat ccgggtcggt atggaaaata caaaccatat aaagccattc caattcatat
     1381 tatgtaaact tcacttctaa cgctacaatt ttttcgtagc ataagaaaaa atactacgac
     1441 aaaatcgcac tgtagccctt gtcttgtccg attcccgcct aatgacgacc gacagggatc
     1501 atttcatgcg cgcaggcact gatttggata ggtgctattc ggtatttgtc ctcgttttca
     1561 aagagcgcca cacaactggc cgggttttgt tcagtaaacc aaaaactaag gatgatcttg
     1621 acccccaatg ccatatggcc acctacagcc gttccattgg caactcgagc tatttcggtt
     1681 ttggcattct gctctgttca caagataagt gcctcgagct accgttgtca atcaccatca
     1741 tccacagcaa agacgcgtgt ttgacgctgg gataagtgaa taagatgtgc cggcagatac
     1801 gttatggagt tgtggtccac aatttcgtaa ggcgcaggca gcttcagcca tccggtctca
     1861 cgacttctgt ggtagtatca ggtggtgggt ttcacttagg tcattaataa tcatcgaggg
     1921 tcttagtgaa atctgtgggt agggataatg ggctttcccc tgctcaacca ctgtatggag
     1981 ggaaatagat tggctaaggg attgaaggat ccggttagcg ggctgactca tcttgcaggt
     2041 gcgatcatgt ccgttatagg ccttgttgta ctgttgcgag caggtattgc caatgaagat
     2101 ctgtggcaga ttgtgtcgtt ttcaatattc ggcgccagcc tgatattgct ttactccgcc
     2161 agcgctgcat atcatttgct caatgtcccg tccagtgtgg aacttgtatt gcgcaagctc
     2221 gatcacataa tgatcttcgt tctcatagcc ggcacataca ctccggtctg tctcggtccg
     2281 ctgcgtggcg ctacgggcta tgtcatactg acgataatct gggctttggc tattgccggc
     2341 gtgttcttca aggtatttta tctcaacgct ccgaggtggt tgtataccgg catatacttg
     2401 gcaatgggct ggcttataat ctttgctaca ggccctttgg tccgtgcagt aggccttcac
     2461 attctcagtt ggctccttat cgggggagtc ttctactcta tcggggcagt gatctacggg
     2521 ctcaagaagc cggatccgtt cccgggccgg ttcggctttc atgagatatt ccacatcttc
     2581 atcctcctcg gcagcctgag ccattttcgt tttatccatt tgcttgcgta gcgtatcttt
     2641 cttgctgcat tggtcttctc ttttt
//
LOCUS       DAOSAI010000337         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_24941_length_2664_cov_2.602146, whole genome shotgun sequence.
ACCESSION   DAOSAI010000337 DAOSAI010000000
VERSION     DAOSAI010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24941_length_2664_cov_2.602146"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLM83_09800"
     CDS             complement(<1..657)
                     /locus_tag="PLM83_09800"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOK71733.1"
                     /translation="MMRLEFVYASLDGVRDVMVGFATDIGLVRDRNEDAYFAADGCYA
                     VADGMGGHRAGEVASEIAIGVVSAECANVDLGSLDMAQCIEKANRSVIEASRRCDELE
                     GMGTTLTVAAFCEGTLRVGHVGDSRAYLIRGDDIIQLTDDHSVVGELLRSGMLTPEEA
                     ECHPQRHAITRALGFSESLEVDIVERQVEPGDVVLLCTDGLHDIVGDNEIAETIKRFH
                     D"
     gene            complement(644..835)
                     /locus_tag="PLM83_09805"
     CDS             complement(644..835)
                     /locus_tag="PLM83_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71734.1"
                     /translation="MVRVALAILRLACVASLYFFLYRVCRALVLDVSFDAGSDNLDDE
                     KMLEGQSAAVPDAGGDDAS"
     gene            complement(843..1934)
                     /gene="rlmN"
                     /locus_tag="PLM83_09810"
     CDS             complement(843..1934)
                     /gene="rlmN"
                     /locus_tag="PLM83_09810"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOK71735.1"
                     /translation="MIKTVDIKNMSFEECRGFFASLGEPAYRADQVFEWLHGKRTASY
                     DEMTNLPVSLRRTLAEVAPLRPPEVLAVHSSPGGATKYLIGLADGNAVETVLMEHDYG
                     LSLCVSSQVGCGMGCAFCASGLEGLVRNLTCGEMIDQILAVSNGIANESPNRRVSSVV
                     MMGSGEPLANYDNVLKFVRLINWKRGYGIGYRHITISTCGIVPGIRRLAQEGLPVTLS
                     VSLHAPDDHTRSALMKINQVYPVGQLIEACIDYANSTGRRVTYEYSLIGGVNDSRDHA
                     LRLARLLGGTLCHVNLIPLNPVPETGFARPSQEAVNVFACTLRSAGIETTIRREMGGD
                     IDAACGQLRRRWAGRPGASDPSKRRDEGG"
     gene            complement(2253..>2664)
                     /locus_tag="PLM83_09815"
     CDS             complement(2253..>2664)
                     /locus_tag="PLM83_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71736.1"
                     /translation="GEHPEAADTVGVNVAGIRYLCVILSGFLAGMGGVTLSLGQLSLF
                     REGMSAGKGFIALAAVIFGRWTPVGAMWASLLFGLADGLQLTAQNWGFTAIPREFLIM
                     TPYIVTMAALAGIIGRSVPPAASGKPYVRRDGGA"
BASE COUNT          626 a          876 c          628 g          534 t
ORIGIN      
        1 gtcatggaac ctcttgatcg tctcagctat ctcattatcc ccaacgatat cgtgaaggcc
       61 atctgtgcac agcaaaacga catctcccgg ctcaacttgc ctctcgacga tatctacttc
      121 gaggctttca gaaaagccaa gtgccctcgt aatcgcatgg cgttgcggat gacactctgc
      181 ctcttcaggc gtgagcattc cagatctgag cagttcgccc accacagagt ggtcgtcggt
      241 gagctgaata atgtcgtccc cgcgaatcag gtacgcgcgg gaatcgccca catgccctac
      301 tctgagcgtt ccctcacaaa aagcagccac cgtcaacgtg gtgcccatgc cttctagttc
      361 gtcacagcgc cgtgaagcct caatcacact gcggttggcc ttctcaatac actgagccat
      421 gtcgagactg ccaaggtcaa cattggcgca ttccgccgag acaaccccga ttgcaatctc
      481 agatgccacc tctccggccc tgtgcccgcc catgccgtcg gctacggcat aacatccatc
      541 cgcggcaaaa tatgcatcct cgttgcggtc gcgcaccaga ccaatatcgg tagcaaaccc
      601 aaccatcacg tccctcaccc catccaggct agcatagaca aactcaagac gcatcatcgc
      661 cgccagcatc gggcacagcg gcagactggc cttccaacat cttttcatcg tcaagattat
      721 ccgaaccggc atcaaaggaa acgtcgagga ccagagccct acaaacacga tatagaaaga
      781 agtagagcga ggccacgcac gcaagcctaa ggatcgccag cgccacacgg accattcaat
      841 ctctaccccc cctcatctcg ccttttgctc ggatcagacg cccctggacg ccctgcccac
      901 ctccgcctca gctggccaca ggctgcgtct atatcgccgc ccatctcacg cctgatagtg
      961 gtttcgatcc cagcggaacg aagtgtgcag gcgaacacat tgactgcctc ctgagaggga
     1021 cgagcgaacc ccgtctccgg cacgggattg agtggtatga ggttgacatg gcacaaagtg
     1081 ccccccaaga ggcgcgccag ccgcagggca tggtccctcg aatcattaac accgcctatc
     1141 agagaatact cgtacgtcac gcgtcttccc gtggaattcg cataatcaat acatgcctcg
     1201 atcagctgcc ccactggata gacctggttt atcttcatca atgcagaccg tgtgtgatca
     1261 tccggcgcat ggagtgatac ggaaagcgtc acaggcagcc cttcttgagc aagcctgcgt
     1321 atgccaggca cgattccgca tgtcgagatg gttatgtgcc tgtagcctat cccgtagcct
     1381 ctcttccagt tgataagtcg cacaaacttg agaacgttat cgtaattggc caagggctct
     1441 ccgcttccca tcataacgac cgaactgacg cgcctgttgg gactttcgtt ggcgatgcca
     1501 ttgctcacgg ccaatatctg gtctatcatt tcgccgcatg tcaggttcct cacgaggccc
     1561 tcaaggcctg aagcacaaaa agcgcacccc ataccgcagc ctacctgaga cgaaacgcac
     1621 aagctcaatc cgtagtcatg ctccatcaag accgtctcaa cagcattgcc atctgccagg
     1681 cctatcaggt atttcgtggc acctccgggc gaggagtgca cggcgaggac ctctggagga
     1741 cgcaagggag cgacctccgc aagcgttctg cggagcgaga cagggagatt tgtcatctca
     1801 tcgtatgacg ccgtccgctt gccatgaagc cactcgaaaa cctggtccgc cctgtaggcc
     1861 ggctccccga gcgacgcaaa aaaccctctg cattcctcaa atgacatgtt ctttatatcc
     1921 acagtcttaa tcattccacc aaatcacaag aaatcctctg tcggcgcact cccgccctcc
     1981 gtaggctacc catgtggcaa cctaggtggc gaacagcagc gcaggttcca tcacatccgc
     2041 tatcaatgaa ggcttgtctg ttctcagtgc cattgcaacg acacaaaggc gaccctatca
     2101 cgggaggggt cgcctctcaa tgccatctgc gccatcacag ccccatctga atggcgtcga
     2161 catgtccaca gcaatactct attgcgcaac ttgaaacgcc tatcaccaag atgcgtccaa
     2221 gccgtatccc gctccaccgc caagcaaagt gatcaggcgc ccccgtcacg cctgacatag
     2281 ggtttcccgc ttgcagctgg cggaaccgac cgcccgatga tgcccgctaa tgccgccata
     2341 gtaacgatgt acggcgtcat gatcaagaac tccctcggaa ttgcagtaaa tccccagttc
     2401 tgtgctgtca gctgcaatcc gtctgccaga ccaaacagga gcgaagccca catggctccc
     2461 acaggagtcc atctgccgaa tatgaccgcc gccaaagcta tgaacccctt gcccgcgctc
     2521 attccctccc tgaagaggga gagttggcca agggataacg tgacgcctcc catgcctgca
     2581 aggaatccac tgagtatcac gcacaagtac ctgataccag cgacgttcac gcctactgtg
     2641 tccgcggcct ccgggtgttc gccg
//
LOCUS       DAOSAI010000338         2641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_25243_length_2641_cov_3.857695, whole genome shotgun sequence.
ACCESSION   DAOSAI010000338 DAOSAI010000000
VERSION     DAOSAI010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25243_length_2641_cov_3.857695"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PLM83_09820"
     CDS             complement(<1..1239)
                     /locus_tag="PLM83_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOK71737.1"
                     /translation="MKFIHTSDWHLGRIFYGVRLTDDQAYVLDQFVDLVRDESPDAVV
                     IAGDIYDRTAPPVEAVQLLDETLSRIVLGLGVPVILIAGNHDSPERLSFGSSLMQTQG
                     LHIIGLPDMCAPIEISDAHGPVWFVPIPYAEPAVVRDRLCAPDAHDHDTAMKAQFEAA
                     AAEAINFATAQYGAVGTGELGEGPGKQKIAWGGMPDAGSPAMRSLFEHSAAADFSPNI
                     VPWNVRAVAVAHAFVSGGTVSESERPLSVGGAGMIDISHFLPFTYTALGHLHRPQTMS
                     ETAVRYSGSLLKYSFDEADHSKSVTVVEIDGDGTPSVRVVSLTPKRDVRKIKGRFEEV
                     MRGPGPGESRHDYISVCLTDREAIWDAMNRLRAVYPNVMQIVPSEDAMADAAESGLVA
                     ARVDHRKVTDLELFCDFYSQV"
     gene            1618..1848
                     /locus_tag="PLM83_09825"
     CDS             1618..1848
                     /locus_tag="PLM83_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71738.1"
                     /translation="MKRVINGLRLAACIAVVVLLFTTTSGCLGGVISQPYSVSGKITD
                     PDGNGSAALQLPSAVGSEPQPPRMMANGPKMV"
     gene            1932..2528
                     /locus_tag="PLM83_09830"
     CDS             1932..2528
                     /locus_tag="PLM83_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71739.1"
                     /translation="MDFVGSKLDYLGLFELSEWNYNVTIEPPPEDELNELYVSVAVDS
                     VEECEDGTYFYISLEGSEVAPAYHVLTGQPEPPEEEVMLAIARKEDGYYLVIQVEGQE
                     STETFLFEAPLEAGDTIMDELLTVEGEESIATPTGTFKALRCALAVSDEDDVERITFW
                     LAPYIGLVKAEIVSSPYPGDENSEAATLTIILESYAAQ"
BASE COUNT          657 a          738 c          686 g          560 t
ORIGIN      
        1 aacctgcgaa tagaaatcgc agaagagctc caggtcggtg actttcctgt gatccaccct
       61 tgcagcaacg aggccagatt cggccgcatc tgccatagca tcctcgctag gcacgatctg
      121 catgacattg gggtagacag cccgcaaccg gttcatggca tcccatatcg cctcccggtc
      181 ggtaaggcag actgatatgt aatcatgtcg cgattcgccg ggccccggcc cgcgcatgac
      241 ttcttcaaac cgtcctttga tcttgcgcac atcgcgtttt ggcgtcagcg acaccactct
      301 cactgacggt gtcccatcgc cgtctatctc cacaactgtg acagattttg agtggtctgc
      361 ctcgtcaaat gagtatttga gcaaagagcc ggagtacctg actgccgttt cagacatggt
      421 ttggggacga tgcaagtggc caagggctgt gtatgtaaag ggaagaaaat gagatatgtc
      481 gatcatgccc gcgcctccaa cggaaagtgg ccgttccgac tctgacactg tcccgccgct
      541 aacaaatgcg tgggcaacgg caacggcgcg aacattccat gggacaatgt ttgggctaaa
      601 atccgcggca gcggaatgct caaaaagtga ccgcatcgct ggagagccag cgtccggcat
      661 tcctccccat gcaatcttct gcttcccagg cccttctccg agctcccctg ttcccacagc
      721 accatactga gctgttgcaa aattgatcgc ctcagcagct gccgcctcga actgcgcctt
      781 cattgcagta tcatgatcat gcgcatccgg agcgcaaagc ctgtcgcgca caacagcagg
      841 ctcggcatag ggaataggca cgaaccacac cggcccatgc gcgtcgctga tctcgatggg
      901 ggcgcacatg tcaggcagcc cgataatgtg tagtccttgc gtctgcatga gcgaagaacc
      961 gaagctgagg cgttcgggcg aatcgtgatt tccggcaatg aggatgacag gcaccccaag
     1021 tcccagcact atccgggaca atgtctcgtc gagaagttgc actgcctcca ccggaggcgc
     1081 agtcctgtcg tatatgtcgc ctgcaatcac caccgcgtcc ggcgactcat cccgaaccaa
     1141 gtccacgaat tgatcgagga cataagcctg atcgtcagtg aggcgaactc catagaagat
     1201 ccgccccaaa tgccaatcag atgtatgaat gaacttcaaa aaacatcccc ccacaacccg
     1261 acagcccgac gccgtccagc ggaaacctcg atattcctta taccctacat gcgagggccc
     1321 aattcccttg tcagacacaa ttacaaggtt atccgccaaa cccaagactc cttccccggc
     1381 tacaactttc ctcgaccaca atgtgcctgc atcagacggc gagcatccat tgcggttccg
     1441 gcgcggcgag gcaacgcgct tgggagataa gtcctagtca aagttgatta tttgatgtta
     1501 tatgttttgg agtggataat taggatatat tcggatatcg ttccgaaata agcggttgtg
     1561 accatgggac tcagtgcttc ctgtggtcaa accacaacgt tgggaggcgt agtattgatg
     1621 aagcgggtaa tcaatgggct tcggctggca gcctgcatag ctgtggttgt gttgctgttc
     1681 actacaacgt cagggtgcct tggaggggtc atttcgcaac cctattcagt gtcaggcaag
     1741 atcactgatc ccgacggcaa cggatcggcg gcgttgcaat tgccttcagc ggtaggttcg
     1801 gaaccgcaac cacctcggat gatggcaaat gggccaaaga tggtctgaag ggcaaagtca
     1861 ccgtcacccc cacaaaggaa ggctggaagt tcacgccagg ttcaaagacc gtttccaaag
     1921 aggcatcgaa tgtggacttt gtgggaagca aactcgatta ccttggcctt ttcgagctca
     1981 gcgaatggaa ctacaacgta accatagagc caccgccaga agatgagcta aacgaactat
     2041 atgtaagcgt agcagtcgac agcgttgaag agtgcgagga cggaacttac ttctacatat
     2101 ccctcgaggg cagcgaagtc gctcctgcct accatgtttt gactgggcag cccgaaccgc
     2161 cagaggaaga agtaatgctg gcgatagccc gaaaagaaga tggctactac cttgtcatcc
     2221 aagtagaggg ccaagaatcg actgagacct ttctgtttga ggcgccgctt gaggcgggcg
     2281 acaccataat ggacgaactt cttacagtcg aaggagaaga atcgatagca acaccaaccg
     2341 gcacattcaa agccttacgt tgcgctctcg cggtttcaga cgaggacgac gttgaacgaa
     2401 tcactttttg gcttgcgcca tatatcggac tcgtaaaagc agagatcgtt agcagcccct
     2461 accctggtga cgaaaactct gaagcagcaa cactcacaat tattctcgaa tcatacgcgg
     2521 ctcaatagac gcggctcatc ttcgctctag gctatgattg atagcagccg tcagtctcgg
     2581 caggacggct gctctttcgg cgctcgttta cgcaccgttc aagaaaaaca tagactggag
     2641 c
//
LOCUS       DAOSAI010000339         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_25478_length_2625_cov_3.256031, whole genome shotgun sequence.
ACCESSION   DAOSAI010000339 DAOSAI010000000
VERSION     DAOSAI010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25478_length_2625_cov_3.256031"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PLM83_09835"
     CDS             <1..1317
                     /locus_tag="PLM83_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOK71740.1"
                     /translation="RGQISGLNQASRNAQDGISLLQTAEGALQETHSILQRMRELAVQ
                     AASDTVTNDDRLEIQKEIDNLRVEMDRISTDTEFNTQKLLNGKFANKIIHIGANAQQN
                     LKVSIASMGARDLKVNKVTTASSATTLNDASFIAEHAGDATLTVSGTYTGQAPITALR
                     TSITEDGKVRVIGKRDGAEGDLIVDLDSDAGVTVDGLTFAIANIADVRAYATTIEDGP
                     TDGKIADESDAGSAAQFSFINGDGATLAMSGTATENITDISATVVGDGQVQISYTIGN
                     DTTRVTLHGDDASATAEHTVAGVTFKLTGAKNVREAATTFTWEDDNLPINAEVVGGLD
                     VVDEGINVSTQAAADAAITVIDEAIVTVSGERSKLGAIQNRLEHTIANLSTSSENLTA
                     AESRIRDVDIAVEMMNFTKYQILSQASTAMLAQANAKPQAVLQLLR"
     gene            1981..2112
                     /locus_tag="PLM83_09840"
     CDS             1981..2112
                     /locus_tag="PLM83_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71741.1"
                     /translation="MKPDGRKDFQDFLDETPICSKLTGNAMAKAAFEELCKYPTSTR"
     gene            2118..2351
                     /locus_tag="PLM83_09845"
     CDS             2118..2351
                     /locus_tag="PLM83_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71742.1"
                     /translation="MSEKGEPALAACISDVEKLLGQQDEFDLNVDFNKQALGRMVKTI
                     LEPFGYSWTGQEKPIRRELSDFITSAAVYSLDF"
BASE COUNT          665 a          639 c          770 g          551 t
ORIGIN      
        1 cgtgggcaga tttcaggcct caaccaggcc agccggaacg cccaggacgg catctccctc
       61 ctgcagaccg cagaaggcgc actccaggag acccactcaa tcctacagag gatgagagag
      121 ctggcagtgc aggcggcgtc ggatacagtg accaatgatg accgtcttga gatccagaag
      181 gagattgaca acctgcgagt cgagatggac agaatctcga cggataccga gttcaacacg
      241 cagaaacttc tcaatggcaa gttcgccaac aagatcatcc atattggcgc taatgcgcag
      301 cagaatctca aggtgagcat agcttcgatg ggcgctaggg atctaaaggt gaacaaggta
      361 accacagcct cgagtgccac tacgttgaac gatgcatcct tcatagctga gcacgctggc
      421 gatgccaccc tcacagtgag tgggacatac actggacaag ctcccatcac ggctctgagg
      481 acaagcatca ccgaggatgg caaggttaga gtcataggga agcgtgatgg ggccgaggga
      541 gacctgatag ttgacctgga tagcgatgcg ggcgtgacag tggacggctt gacgttcgcc
      601 atagccaaca tcgcagatgt gagggcctat gcgacgacga tagaggacgg gcccaccgat
      661 ggcaagatag cagatgaaag tgatgcgggg agcgcggcac agttctcatt cataaacgga
      721 gatggcgcga ctctcgcgat gtctggcacg gccacggaga atataacaga catctctgct
      781 actgtggtcg gtgacgggca ggtgcagatc tcatacacga ttggcaacga tacgacgcgt
      841 gtgacattgc atggtgatga tgctagcgct actgccgagc atacagtcgc aggggtgacg
      901 ttcaaactaa ccggtgctaa gaatgtaagg gaagccgcga ctacgtttac ctgggaagac
      961 gataacttgc cgatcaatgc cgaagtagtg ggcggtctag acgtagtaga cgaaggaatc
     1021 aatgtgtcga ctcaggctgc agctgacgct gcaatcactg tgatcgacga agctatcgta
     1081 accgtttcgg gtgagcgatc aaagctcggt gctattcaaa acaggcttga gcacaccatc
     1141 gcgaacctat cgacttcgtc tgagaacctg acggctgccg aaagccgcat ccgcgacgtc
     1201 gatatcgcag ttgagatgat gaacttcacg aagtatcaga ttctgagcca ggcgtcgacc
     1261 gccatgttgg cccaggccaa tgcgaagccc caggcagtac tgcagctgct gaggtagcgg
     1321 gggaagtcac gtagttgtag tggtatggtg gacgatatgg ggcggccttc gggctgcccc
     1381 tcatagttct cttgcatcta tgcggggtag tcggtggtag aggatatcgc cgttcccatg
     1441 gagttgatga atagatcgca atgtgcacta gagaagctgc cgatcacagg tttgaaactg
     1501 gtcttgcggt ctagacaact ccgctttgcc cggccgccgt cctcagcaga gactgcatgc
     1561 tggtgtcaag ccttactaaa ccggtttttg gcgattccta ttactgtatc ttggtgagag
     1621 tacttgcgct gcagcaaacc tgcccatttc gtcaaatgaa cccatgatgt gatccctgca
     1681 gttgaggatg gcatctatcc actctttggc aaacgagatg cttgcgcaga catggttgtg
     1741 tggtgagtct gtatcggctt ctccggggaa agactggcgc tctggaacta gattagcatg
     1801 tgccgggcgg gatttcacac agtcccgcat tgcaatcatt gtgagcctgc tacccatgat
     1861 gtaggcagag gactgggcat tgggatctga tgattgggga ccgtatagca aagaactgct
     1921 gacctgagcc cggaaaaacg cgcaatggtg gacgcgtaat ctatgcatgg aggcgatact
     1981 atgaagcccg atggaaggaa ggatttccag gactttctgg atgaaactcc gatctgctcc
     2041 aagcttacag gcaacgccat ggccaaggca gcctttgagg aactgtgcaa gtacccaaca
     2101 tctacaagat gattgccatg tctgagaaag gcgagcccgc cctcgctgcg tgcatatcag
     2161 acgttgaaaa actgcttgga cagcaagatg agttcgatct taatgttgac tttaataagc
     2221 aggctctggg caggatggta aagaccatac tcgagccatt cgggtactct tggacggggc
     2281 aggagaagcc cattcgaaga gaattgtcgg atttcatcac atctgctgca gtgtattcac
     2341 tggactttta agatcagcca aaactcgatc ttgtcgttgg gcctttgatg gcgaacataa
     2401 agaggaatgc cttaacggca aatctagccg ggattcacgg tccgtgtcac ctgtgccctt
     2461 ttaagcgcag gtgacacctt gttcgcccgg catgtttgtt gagccgatgg actgaaagaa
     2521 gtcctgtcac ccaaccagcg ggaagacaac ctcaaggcaa gggcgtcgct gaaagcggcg
     2581 gggtctgaag gaagctttaa gggggaacac ggaacgtagc gaaag
//
LOCUS       DAOSAI010000340         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_25481_length_2625_cov_2.954475, whole genome shotgun sequence.
ACCESSION   DAOSAI010000340 DAOSAI010000000
VERSION     DAOSAI010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25481_length_2625_cov_2.954475"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1330
                     /locus_tag="PLM83_09850"
     CDS             32..1330
                     /locus_tag="PLM83_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK71743.1"
                     /translation="MTVAVAGGGWAGCAAAVAAAQAGADVVLIERTNLLLGTGLVGGI
                     MNNNGRFTAARELVSMGGGYLLDIIDQATLHRRINFPGHLHASLYDVAKIESRVREGL
                     ESLGIHVMLESRVTDVEMNGRRIAHLVLADGSRIGADAFVDATGTAGPQGNCARVNGN
                     CVMCILRCPAFGPRVSIAGKAGANEISGSLFEAMSGSCKLEKKSLSVWLVRSLEENGV
                     LVVPVPRYIARLDKTASKACQQYSLPEFYDNLVVLDTGHAKLMAPFFPLDCLRRIPGF
                     EHAVFHDPYAGGRGNSIRYNIITPHDQAMKVEGIDNLFCAGEKVGLLVGHTEALCTGS
                     LAGNNAVRSCVGQESLVIPQSLAIGDLIAFIEQSLRTSTGFLNKYTFAGGVYFRRMYD
                     SGLYTTDVRRINQRVKAAGMDGVFNRKLVQDKHRTGWLPT"
     gene            complement(1573..2436)
                     /locus_tag="PLM83_09855"
     CDS             complement(1573..2436)
                     /locus_tag="PLM83_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOK71744.1"
                     /translation="MTTLRRLTACNNRFGRSMNTSQVRPWAAIVCVFAMILACSIPVC
                     GQPKVPAKPVPPRLMNDLAGMLTSSEVDDIEERLVALNRANGVQFAVVIVPDMGGYSI
                     EEYSVELFQKWGIGEAKEDNGLLLLIAEDEGRLKFETGYGLEGDLPDSFLGRVIDENL
                     VPHFVEGDPAAGIVEAINTIALRLTGEEMEPAEEESKGLSPDALAAIVFFAIVIAFVI
                     AKSSARKNRSKRPPRSGGGVYRPPSTPRPFSGGGWKGPSGGSGGSSSGGGFGGFGGGK
                     SGGGGASGGWK"
     gene            complement(2487..>2625)
                     /locus_tag="PLM83_09860"
     CDS             complement(2487..>2625)
                     /locus_tag="PLM83_09860"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOK71745.1"
                     /translation="NTSVQSYNVKIRTFPNSIVAGMRGAVPKDMFEAQAGALVAPEVK
                     F"
BASE COUNT          597 a          763 c          706 g          559 t
ORIGIN      
        1 agctttcaag gggggaggca tcacgatgag cgtgacggtg gcagtcgcag ggggcgggtg
       61 ggcgggatgc gcagctgcag tcgctgcagc gcaggcaggc gcagacgtgg tgctcataga
      121 acgcaccaat cttcttttgg ggaccggcct cgtaggcgga ataatgaaca acaacgggcg
      181 attcactgcc gcacgcgagc ttgtctcgat gggaggaggc tacctcctcg atatcataga
      241 ccaagctaca ctgcatcgca gaatcaactt tccaggccac ctacatgcaa gcctgtatga
      301 tgtagcaaag atagagtcgc gcgttcgaga gggcttggag agcctcggga tacacgtgat
      361 gcttgaatct cgcgtcacag acgtcgagat gaacggaaga cgcatagcac acctggttct
      421 ggcggacgga agccgtatag gagcagatgc attcgtggat gccacaggaa cagctggacc
      481 gcagggcaac tgcgcccggg tcaatggcaa ttgcgtaatg tgcattctca gatgccctgc
      541 cttcgggcca cgggtcagca ttgcgggtaa agccggggca aacgaaatct caggaagcct
      601 tttcgaggcc atgagtggat cctgcaaatt ggaaaagaag tcgctatccg tgtggctagt
      661 aagaagcctc gaggaaaacg gcgtgctcgt ggtcccagtc ccgcggtaca tcgcacgcct
      721 ggacaagaca gcatcgaagg cgtgccagca gtattcgctc ccagaattct atgacaacct
      781 ggtcgtcctc gacactgggc acgcaaaact tatggcgccc ttcttccccc tcgattgcct
      841 caggagaatc cccgggttcg aacacgcggt atttcatgat ccctacgcag ggggacgagg
      901 caactctata agatacaaca tcataacccc tcacgatcag gctatgaaag tggagggcat
      961 cgacaacctc ttctgcgccg gagagaaggt aggcctattg gtgggacaca ccgaagctct
     1021 ttgcaccggg tcacttgccg gcaataatgc tgtgcggtca tgcgtagggc aggaatctct
     1081 cgtaattccc caatctctgg cgataggaga cttgatagcc ttcatagagc aatctctccg
     1141 aacgtccacg gggttcttga acaagtacac ctttgccgga ggcgtctact tcaggcgcat
     1201 gtacgactca ggtctctata cgactgacgt ccggcgcatc aaccaacgtg tgaaggccgc
     1261 cggcatggat ggcgtgttca accggaaatt ggtccaagac aagcatcgaa cagggtggct
     1321 gcccacataa cgctgagcgg ccctcgggct tctagatggc cttggcattt gcattgcagg
     1381 ccgctcagcc tacacctgct acagatatct ggagctcgta gccggaaacg aatcgatcag
     1441 tgcgtgcgag gcaagagaac actttatccg ttgaaccagc tctggcgctc gaggcgacct
     1501 ctgcccttgt tgatcccgtc aaactagtag cgggaagacg tcagccctaa tgcgctattt
     1561 gacgccgttg cgctatttcc atcccccgct ggcgcctccg ccgcccgatt tgcctcctcc
     1621 gaacccgccg aatccgcctc cggaggaact acctccagaa cctccagacg gtcccttcca
     1681 tcctccacca gagaagggtc taggagtaga aggaggccga tacacgccac caccggatct
     1741 gggcggtcgc ttactgcggt tcttgcgtgc gctggacttc gcaatcacaa atgcgattac
     1801 aatggcaaag aagactatcg ccgccagcgc atcaggagaa agccctttgc tctcttcctc
     1861 agcaggttcc atttcttcgc cagtaagcct gagagcgatc gtgttgattg cctccacgat
     1921 gcctgccgcg ggatcgcctt ccacaaaatg aggaacaagg ttctcgtcga tcacgcggcc
     1981 caagaacgag tcaggcagat cgccttcgag gccataacct gtctcgaact taagccttcc
     2041 ctcgtcctct gctattagca aaagcaggcc gttgtcctct tttgcctctc ctattcccca
     2101 tttctgaaag agttcaaccg agtattcttc tatcgaatag cctcccatat ctggaacgat
     2161 aacaactgca aattgcaccc cgttggctct gttcagcgcc acgagtcttt cctcgatatc
     2221 atccacttcc gaagacgtaa gcatcccggc caagtcattc atgagccgcg gcggcaccgg
     2281 ttttgcagga acctttggct gcccgcacac aggaatggag caagccagta tcatggcaaa
     2341 gacgcaaaca attgccgccc atggcctcac ctgggatgtg ttcatcgatc ggccgaatct
     2401 gttgttacac gccgtaagcc ttctcagtgt tgtcatttgc atccttcgct ccgatctgtt
     2461 cccgtctggg tcatccccgc acatccttag aacttgacct ctggagcaac caacgcacca
     2521 gcctgggctt cgaacatatc cttggggaca gcgccgcgca ttcctgcaac tatcgagttg
     2581 gggaaagtgc ggattttcac gttgtatgac tggacggagg tattg
//
LOCUS       DAOSAI010000341         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_25661_length_2611_cov_4.182316, whole genome shotgun sequence.
ACCESSION   DAOSAI010000341 DAOSAI010000000
VERSION     DAOSAI010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25661_length_2611_cov_4.182316"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PLM83_09865"
     CDS             complement(<1..1096)
                     /locus_tag="PLM83_09865"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOK71746.1"
                     /translation="MAKVIDWTIAIFGQYGLVGLFVVSFAESSFFPVPPDVMLIPLCL
                     ARPRASMLYATVATVSSVLGAVFAYLVGARGGRPLLLKFTSKDGLSKIQGLFKRFGGW
                     AVGIAAFTPIPFKVFTIASGIFQVRLAPFLIASTLGRGARFFLEAALVLKPGEQVRDI
                     IGTQFELITLMVGLVVAVAAIVWNRKSSVQAPIFSDKRGSGTKPYWRIRLPKSAASPG
                     LSGKRFWAEWGIYAIAGFVFLLIFFEELVELAQPDHAPFLRQIDMGIMAWIRAIRNPF
                     LSTTLTFTATLGSYPFVFACLALAVTYNLFRHRRARAATLAICVIGGLGLAVLTRWAY
                     SLPGPELALPVPDILSRSFAAQPIPVSLPFY"
     gene            complement(1153..2025)
                     /locus_tag="PLM83_09870"
     CDS             complement(1153..2025)
                     /locus_tag="PLM83_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71747.1"
                     /translation="MEKGYDTQGRVDYGQIPEEQRGINSYASDNDTGSSCLKRAAKPR
                     SPLYTILVLAAIMVAMITYTRGDPRGRVVITPPWQPGEVSVLEIRDSHGQLIAAWELR
                     IDSDGKATLFTSDQHGSTYSEHAMVAADPDTLVPIRTELTYESDHGRIVYAALYGEDG
                     VSIEASVPGQNEESIVELPDTPYFDNEQFIMVIRALPLKRNFKATLKDVITRAAMKTT
                     ITLNVAKKEKLEVPAGSFETWKVDLMGTGRTVWIAVPHPHQIVRFEDRKARTTADLVQ
                     YTPGVELETGSEFE"
BASE COUNT          589 a          689 c          723 g          610 t
ORIGIN      
        1 cgtagaaggg cagcgatacg gggatgggct gggcggcgaa gctgcgggac aggatatcgg
       61 gaaccggaag cgcaagctct ggtcctggaa ggctgtacgc ccagcgggtg agaactgcta
      121 agcccagccc gccaatgacg cagatggcaa gggtggcggc tcgtgcgcgc ctgtggcgaa
      181 aaaggttgta ggttactgcc agagccaggc aggcaaagac gaagggatac gatccgagag
      241 tggcggtgaa tgtgagcgtt gtgctgagaa atgggtttcg aatggcgcgg atccatgcca
      301 taatgcccat atctatctgc cgcaagaatg gtgcgtggtc aggctgtgcg agttcgacga
      361 gctcttcgaa gaatatgagc aggaagacga atcccgcaat ggcatatatc ccccactcag
      421 cccagaacct cttgcccgac aaaccggggc tggcagcgga cttgggcaga cgtatccgcc
      481 aataaggttt tgtcccggaa cccctcttgt cgctgaaaat gggcgcttgg acggaagact
      541 tcctgttcca tactatggct gcgacggcca cgaccaggcc gaccatgagg gtgatcaatt
      601 cgaactgtgt tccgataata tcccttactt gttcgccagg cttcaagact agcgccgctt
      661 cgaggaagaa ccgggcgccg cggccgaggg ttgaagctat gagaaagggc gcgagtcgca
      721 cctgaaagat gccggaggct atggtgaaca ccttgaaggg tatcggggtg aaggcggcta
      781 tccccactgc ccagccgccg aatcttttaa atagtccttg aattttgctg agaccgtcct
      841 tgcttgtgaa cttgagcagt agtggccgtc cgccgcgcgc tccaacaaga tatgcaaata
      901 cggcgcctag caccgatgag actgtggcaa cagtcgcata caacatcgaa gctcgagggc
      961 gcgccaggca tagcggaatg agcattacgt caggggggac aggaaagaag ctcgattccg
     1021 cgaatgagac cacaaaaagt cccactaatc cgtactgccc aaaaatcgct attgtccagt
     1081 caatgacctt tgccatagtt caacccacca atgtgttctt tcctctcagg acaggccttt
     1141 gtcacttgtc cactattcga attctgagcc tgtttccagc tccacgcctg gcgtgtattg
     1201 cacgaggtcc gctgtggtcc gggctttccg atcctcgaac cttacaatct gatggggatg
     1261 gggaaccgca atccagaccg tgcgtccagt gcccatcagg tcaaccttcc acgtttcgaa
     1321 cgaaccggcg ggaacctcca atttctcctt cttcgcgacg ttcagcgtta tcgtggtttt
     1381 catcgctgcc ctggtgatca catccttcaa ggtggccttg aagttgcgct tcaggggaag
     1441 ggcgcgtatg accattatga actgttcatt gtcgaagtac ggggtgtcgg gcagttctac
     1501 aatggactcc tcgttctggc ctggaaccga tgcttctata gatacaccat cttcgccata
     1561 caaagcagca tagacaatgc ggccatggtc ggattcgtac gtcaattcgg ttctgatggg
     1621 aacgagcgta tcaggatctg cggccaccat tgcatgctcg ctataggtgg atccatgctg
     1681 atccgaagtg aacagggtag ctttgccgtc tgaatctatt ctcagttccc acgcggctat
     1741 gagctggcca tgggaatcgc gaatctcaag cacactcact tccccgggtt gccagggcgg
     1801 agtgatcact actcgtccgc gcgggtcgcc cctggtgtat gtgatcattg ctaccatgat
     1861 tgctgcaagc accaagatgg tgtacagggg cgatctgggc tttgcagccc tttttaaaca
     1921 gcttgatccc gtatcgttgt cagatgcata gctgtttatt cctctttgtt cttctggaat
     1981 ctgaccatag tcgacacgcc cctgcgtgtc atatcctttc tccacctagt tccactcctc
     2041 tcgctactga acagactgca tatgtggtcc tgcacaatca gattcccaag gctgaatact
     2101 tcctgaaatc cgatcttccc cttggccttt aggcgaaata cgattctcaa ggcccactag
     2161 ttgggctgcc caaagcgggc aaacgcttac cccaatgata cctttatgaa cgcccacgct
     2221 caatacctga tgcattttgg gttgccgggg cgtatgcgac gacttatcag tagatggggg
     2281 aagtattatt ctccagccgc cacaggcatg ctgaggcgca gcccgaatct atgacattga
     2341 gtgatgacga cttgggcgtg atctagaagc ttattctacg acaaatggct tggaaccttt
     2401 ccggcgagcc tgtaaaaagg cggaatgaag tgcaagagcg aatgattagt tatgagaggc
     2461 ttgtctgcct tgaaggggtg ccgaagattt gcgtctaggt aactcccgat aaggttctgc
     2521 gctaccggtt agcctgccat atgatgtctg ctaggagtag acacgtcttt gcacctatgt
     2581 cgcagtgcgg taattggttc gcatttgtgc a
//
LOCUS       DAOSAI010000342         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_25698_length_2609_cov_3.371966, whole genome shotgun sequence.
ACCESSION   DAOSAI010000342 DAOSAI010000000
VERSION     DAOSAI010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25698_length_2609_cov_3.371966"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLM83_09875"
     CDS             complement(<1..385)
                     /locus_tag="PLM83_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOK71748.1"
                     /translation="MMRSMFAGVSGTRAHQVRMDVIGNNIANVNTVGYKYGRATFQDM
                     LSQTMRGSMAPTEKRGGVDPMQVGMGVSTRSIDIVFTPGNLEYTGRLTDMAIQGSGLF
                     VVRDSSGGLMFTRDGAFKIDAEGNLT"
     gene            complement(481..894)
                     /locus_tag="PLM83_09880"
     CDS             complement(481..894)
                     /locus_tag="PLM83_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HOK71749.1"
                     /translation="MTVDRTDMIAGRGMTPGISGVRPDRPAPAGTEVPLARGAGATFQ
                     DVLAEQLSPGGVKFSAHARSRLAARRIDLGQEDLSRLEGGVSRAAAKGARESLILLDD
                     KAFVVSVRNRTVITAVDGEHLKENVFTNIDSAVIV"
     gene            complement(907..1311)
                     /locus_tag="PLM83_09885"
     CDS             complement(907..1311)
                     /locus_tag="PLM83_09885"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOK71750.1"
                     /translation="MYTSRAASVGTYAVPPENTPRKTLGKDEFLMLLITQMRNLDIGE
                     GSSNQEFVAQMAQFTLLEEVQNLSYSVQELMCRQLIAEAGSFVGKDIEAWIPGYDMPV
                     KGHVDSVEVIGGLPYLVVGDMRINMAYVTKIS"
     gene            complement(1326..>2609)
                     /locus_tag="PLM83_09890"
     CDS             complement(1326..>2609)
                     /locus_tag="PLM83_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOK71751.1"
                     /translation="PEASLSAPSGGGVGLGMTSGVWGRGIDSRLAEERPMGVEQEGVS
                     HGSDGVDWAGTSNPKGIGGADEGKPERAELTEVESGAKARAGHGVSSRLHSDAEAAVS
                     TAGSKLEAWRIQIADSQSMAERATSDAGLIKSDGFGVRPAGRQNIVSGMDEGEESLAA
                     REEADGADAADGESVLRVRVPEPDVTMRRDNTSSDMQSDARSRERSHGSREVVFEPRR
                     ESAQSGSEQIGHSDNGLVGFEGLVANLEAVNTGAAPTEGVPIDGRNRISDEAGRSMMR
                     QIAAHIEARRGVGSGEFRAKLVPESLGEVRIRIKVQGGVCTATIRVGDSEVGEYIEEH
                     GTDLKVLLAESGIDLADLSVSVGSHSTSEGAREWTQGQYAAAAFAGGGRRGRKDYAPV
                     EEWFCNSHRNELAPQEIELAAAYARSGHGRIDYFA"
BASE COUNT          499 a          891 c          601 g          618 t
ORIGIN      
        1 gggtgaggtt tccttcggca tctatcttga acgctccatc cctcgtaaac atcaggcctc
       61 cactggaatc gcgaaccaca aacaatccgc tgccttggat ggccatatca gtcagtcttc
      121 ccgtgtattc caggtttccg ggggtaaaca cgatatcgat cgatcttgtg gaaaccccca
      181 tgccaacctg catcggatcg acccctcccc ttttttcagt aggcgccatt gaacccctca
      241 tggtctggga gagcatgtcc tggaaggttg cccttccata cttgtatcca acggtgttga
      301 cgttggctat gttattgccg atcacgtcca tgcgcacctg atgagcgcgg gttccggaca
      361 caccggcaaa cattgaacgc atcattcacg ttcgacctcc atatctcttt tttccgcagg
      421 ccgtctcagt cccagacggt ccagggcttc ctcatagagg tccggcccaa attagcggat
      481 ctagactatc accgcagaat cgatattggt aaacacgttc tccttgaggt gttcgccatc
      541 gactgcagtg atgactgtgc gatttctcac gcttacgacg aatgccttgt cgtccaaaag
      601 gattaaagat tctcgcgcac ccttcgcagc tgccctgctc acgccaccct ccagcctcga
      661 aaggtcctct tggcccagat ctatcctgcg ggcggccagc ctggagcgtg catgggctga
      721 gaatttgact ccgcctggag acagctgttc tgcaagcaca tcctggaagg ttgctcccgc
      781 gcccctggct aagggcacct cagttcccgc tggcgccggc ctgtccggtc gcaccccgga
      841 gatacccggg gtcataccgc gaccagctat catgtctgtc ctgtccactg tcatctgtcg
      901 acacccctaa gatatcttcg tcacatacgc catgttgatc cgcatgtccc caactaccag
      961 gtaaggcaat cctccgatta cctccaccga gtccacgtgc cctttgacag gcatgtcgta
     1021 gccgggaatc catgcctcga tatcttttcc cacaaaggac ccggcctctg caatcagctg
     1081 cctgcacatg agttcctgca ctgaataact tagattctga acttcctcca agagggtgaa
     1141 ctgggccatc tgcgccacga actcctgatt tgaagatcct tcgcctatgt caaggtttct
     1201 catttgtgta atcagaagca tcaggaactc gtccttaccc aaggttttcc tgggagtgtt
     1261 ctccggagga accgcatatg tccctacgct cgcagctctg gaagtgtaca atcctcatcc
     1321 ccccttcaag caaagtagtc gatccttcca tgcccggatc ttgcataagc cgcagcaagc
     1381 tcgatctcct gcggcgcgag ctcatttcta tgggagttac agaaccactc ttcaacaggc
     1441 gcgtagtcct tgcgtcccct gcgcccgccg cctgcgaaag ccgcggcagc gtactgtcct
     1501 tgcgtccact cccgcgcgcc ctcagacgta gagtgcgatc ccaccgatac tgagaggtcc
     1561 gccagatcaa tgccactctc tgcgagaagg actttgagat ctgtcccatg ttcttctatg
     1621 tactccccaa cttcactatc gcccacgcga atcgtcgccg tgcagactcc gccctggacc
     1681 tttatcctta tccgaacctc tccaaggctt tcaggaacga gcttcgccct gaactctccg
     1741 gatccaacgc cgcgcctggc ttcaatgtgg gcagctatct gccgcatcat cgaacggcca
     1801 gcctcatctg atatgcggtt tcgtccatct atgggcacgc cttctgttgg tgccgcgcct
     1861 gtgttgactg cttccaagtt cgctaccagc ccctcaaacc caacaagtcc attgtcggaa
     1921 tggccgatct gctcagatcc ggattgtgcc gactcccgcc ttggttcaaa gacgacctcg
     1981 cgactgccgt gcgatcgctc tcgcgaacgg gcatccgatt gcatgtccga gcttgtatta
     2041 tcccgcctca tagtcacatc cggctccggt acacgaaccc gcagtacact ctcgccatca
     2101 gcagcatcgg cgccatccgc ttcctccctc gctgccaggc tttcctctcc ctcgtccatc
     2161 ccgcttacga tgttctgacg ccctgcaggt ctcaccccaa acccatcgga tttgattagc
     2221 ccggcatcgg atgtcgctct ctctgccatc gactgagagt cggctatctg aatccgccaa
     2281 gcctcaagtt ttgatcccgc agtgcttacc gcggcctccg catcactgtg aagtcgagaa
     2341 gaaaccccgt gtccagctcg tgccttggca cccgattcga cctctgtcaa ctcggccctt
     2401 tccggcttcc cttcatcagc gcccccgatt cctttgggat tagacgtgcc cgcccaatcg
     2461 actccgtcag agccatggct cacgccttcc tgctccacgc ccatgggcct ttcctctgct
     2521 aaacggctgt cgatgcctcg gccccagacc ccgctggtca ttcccaggcc gacacctccc
     2581 ccgctcggag cagaaaggct ggcttccgg
//
LOCUS       DAOSAI010000343         2603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_25779_length_2603_cov_3.236264, whole genome shotgun sequence.
ACCESSION   DAOSAI010000343 DAOSAI010000000
VERSION     DAOSAI010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25779_length_2603_cov_3.236264"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1100
                     /gene="ftcD"
                     /locus_tag="PLM83_09895"
     CDS             204..1100
                     /gene="ftcD"
                     /locus_tag="PLM83_09895"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOK71752.1"
                     /translation="MQQVVECVPNFSEGRRPEVVREIVAAIEAVSGIRVLDYSSDTSH
                     NRTVVTFVGTPEACEEAAFAGIARACDLIDMAKHHGEHPRLGATDVVPFIPISGVTME
                     ECVEIARRVGQRVGEELEIPVYLYEEAATRPERRNLADVRRGEYEGLKESIGTPERAP
                     DFGPARVHPTAGATIIGARMPLVAFNVNLGTSDIGIAKKIARAVRESSGGLANVKALG
                     VMLEDRQIAQVSMNLVNYVKTPIPRVMEMVRREADRYGVSVVESELIGLMPMDAVLDT
                     AAYYLQLPKLEQSQIIEARVWE"
     gene            1196..2590
                     /gene="hutI"
                     /locus_tag="PLM83_09900"
     CDS             1196..2590
                     /gene="hutI"
                     /locus_tag="PLM83_09900"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOK71753.1"
                     /translation="MGKMGAGFRPRTEPSDLLIIHAAELGTCRARAAWDNGRPEPGPR
                     LGADLGCAGIIEDGAIAVRDGVIVDIGATEEVLSREPEWLARRPNGAGQVADFPEAIR
                     SVTEVFDASGRTVVPGFVDAHTHLVYAGSRYREHLMRLEGAEYLDILKAGGGILATLE
                     ATRKASFDELLELALKRLDVMLLHGTTTVEAKSGYGLDLDTELKQLRVARAACDDHPI
                     EIVSTFMGAHAVPPEFTGDPDGYIDFMIAEVLPQVAEGGLAEFCDVFCEEGVFSVEQS
                     RRVLMAGLAHGLCPKMHADEIYPIGGAELAGELGAASADHLAAASKAGMESLAHAGVV
                     AVLLPGTMFTLMSERYANAREMINLGVPVALATDYNPGTSPVMSMQFVMTLACLKMGM
                     TPQEALCAATANAAYAVGRGGDAGVLRVGAAADFIVCDVPAVDALPFSVGVNPVTDVF
                     VGGVRVVSDRVLPY"
BASE COUNT          544 a          638 c          863 g          558 t
ORIGIN      
        1 gcgctcgaat cgccgtttct cggagtgcgc gggctagaag gaattgccgg gctcacggag
       61 aaagcgtatg cggtaacata caggtctttg ttccccagaa ggtagaatgc aacgcctcgt
      121 gaggggtcgc tcctcgcgca ggggtgtgga ttggaattgt aggtttttca tgcaggaggt
      181 gccataagtt gagtgataga actgtgcagc aagtagtgga gtgtgtcccg aatttctcgg
      241 aaggcaggcg gcctgaggtt gttcgggaga tcgttgcggc catagaggcg gtttcaggga
      301 tccgtgtgct ggactattcg tcagatacca gccacaatag gactgttgtt acattcgttg
      361 gaacgcctga agcctgcgaa gaggcggctt ttgcaggcat tgcccgcgcg tgcgacctga
      421 tcgacatggc aaaacatcac ggagaacacc cccggctcgg ggcgaccgat gtggtgccgt
      481 tcatacctat ttcaggtgtg accatggaag agtgtgtcga gatcgcgcgc cgcgtcggac
      541 agcgtgttgg cgaggaactt gagataccgg tctatctgta tgaagaagcg gccacgcggc
      601 ccgaaaggcg caatttggct gacgtgaggc gcggggaata tgaggggctc aaggagagca
      661 tagggactcc tgaacgcgcg ccggatttcg gccctgcgag ggtgcacccc actgcaggtg
      721 ccactataat cggcgctagg atgccgctcg tagctttcaa cgtgaacctg ggcaccagcg
      781 acatagggat cgcgaagaag attgcccgcg ccgtccgcga atccagcggc ggtctcgcca
      841 atgttaaggc gctcggtgtt atgctcgagg acaggcagat agcgcaagtc tcaatgaact
      901 tggtgaacta cgtgaaaact cccatccccc gcgttatgga aatggtgaga cgcgaggcgg
      961 atagatacgg ggtttcggtg gtcgagagcg agctcatagg cttaatgccc atggatgctg
     1021 ttctggatac tgccgcctac tatctgcagc tgcctaaatt ggagcagagc cagataatcg
     1081 aggcgcgggt ttgggagtag gggcggcagg cttcgactgc gcggccgcaa gatacggcag
     1141 agactgagtg tgcttggcga acaaggcatt gctgaggcaa ggagggctct ttatcatggg
     1201 caagatgggg gcgggctttc gccctaggac tgaaccatcg gacttgttga tcatacacgc
     1261 agctgaactc ggaacatgta gggcacgggc tgcttgggac aatggccggc cggagcccgg
     1321 gcctagatta ggcgcagatc ttggctgcgc aggaattata gaggatggag cgattgctgt
     1381 aagagacggc gtgattgtgg acatcggtgc cactgaagag gtgctgtcgc gtgaaccgga
     1441 atggctagcc cgaagaccaa atggagccgg gcaggtggcg gactttccgg aggctatccg
     1501 ttcagtgacc gaggttttcg atgcaagcgg caggacggtg gttcctggat ttgtggatgc
     1561 gcatacccat cttgtttatg ccgggagtcg ctacagggaa cacctgatgc gtcttgaagg
     1621 ggccgagtac ttggacatcc ttaaggctgg cggcgggatc cttgcgactt tggaggcgac
     1681 tcggaaagcg tcctttgacg aactgcttga gcttgctttg aagcggcttg atgtaatgct
     1741 gctccacggg actacgacgg tcgaggcaaa aagcggttac ggcctcgatc tcgacacaga
     1801 actgaagcaa ctgagggttg cccgtgccgc ttgcgacgat catccgatag agatagtcag
     1861 cacattcatg ggggctcacg ccgttccccc tgagttcaca ggcgacccag acggttacat
     1921 cgatttcatg atcgccgagg tccttccgca agttgccgag ggagggttgg cagagttctg
     1981 tgatgtcttc tgcgaggaag gggttttctc agtagaacag tcgaggcggg tgctaatggc
     2041 aggccttgcc catggactct gtcccaaaat gcatgctgac gagatatacc ccataggcgg
     2101 agcagagctt gcgggagagc ttggggcggc atcagctgac caccttgccg ctgcgtccaa
     2161 agctggcatg gaaagcctgg ctcatgcagg ggtggtggcg gtgttgttgc cgggcacaat
     2221 gttcacgttg atgtccgaga gatacgctaa cgccagggaa atgataaacc tgggagttcc
     2281 tgttgcgctc gccactgact acaatcccgg gacatcgcct gtaatgtcaa tgcagtttgt
     2341 gatgaccctt gcctgtctca agatgggcat gactccccag gaagcgctat gcgccgccac
     2401 ggccaatgca gcctacgcag tgggaagggg aggagacgca ggcgtcctcc gggtaggggc
     2461 agccgccgac ttcatagtct gtgatgttcc tgccgtagat gccttgccgt tttcggtggg
     2521 agtcaacccc gttactgatg tgttcgtagg gggcgtgcgt gtcgtatccg accgtgtgct
     2581 tccctattga cttttctagg agg
//
LOCUS       DAOSAI010000344         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_25811_length_2601_cov_3.136292, whole genome shotgun sequence.
ACCESSION   DAOSAI010000344 DAOSAI010000000
VERSION     DAOSAI010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25811_length_2601_cov_3.136292"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLM83_09905"
     CDS             <1..439
                     /locus_tag="PLM83_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOK71754.1"
                     /translation="AKGFASCDHLVIICGHYEGVDERVMSLVTDRVSIGDYVLTGGEL
                     PAMVIVDATARLLPGVLGDEESASADSFWDGILDYPHYTRPRVWRGHEVPDVLLSGDH
                     ERIRKWRRKQALKVTLEQRPDLLSQVELTDEDVELLKQVMEDG"
     gene            536..883
                     /gene="rplS"
                     /locus_tag="PLM83_09910"
     CDS             536..883
                     /gene="rplS"
                     /locus_tag="PLM83_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOK71755.1"
                     /translation="MNIIDTIEREQMRSDLPDFGPGDTVRVHVRVVEGARERIQVFEG
                     VVIGRSGSGLSETFTVRKMSSGIGVERIFPVHSPKIDKVEVVRQGDVRKAKLYYIRGR
                     VGKRARIREKRQH"
     gene            1127..1690
                     /gene="lepB"
                     /locus_tag="PLM83_09915"
     CDS             1127..1690
                     /gene="lepB"
                     /locus_tag="PLM83_09915"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOK71756.1"
                     /translation="MEYVNACIVAVVLAAFIMTFVARSFVVQGSSMEPTLYNGERLLV
                     NRFIYRFREPQRGDIIVFRYPQDPRQMFIKRVIGVPGDELMISGGMVYVNGTGLNEPY
                     IAEPSTREYGPALVPTKRVFVLGDNRNNSQDSTDDSVGMVPFGSIQGKAFVVYWPVDR
                     IRTLKNPTCLEAIGTPESGMAAAPGMP"
     gene            1758..2600
                     /gene="ylqF"
                     /locus_tag="PLM83_09920"
     CDS             1758..2600
                     /gene="ylqF"
                     /locus_tag="PLM83_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOK71757.1"
                     /translation="MGFSWYPGHMAKAVSQIKQSMKLIDVVIEILDARAPRSTENREF
                     AEAVGNKPRIVVLNKADLADPVATRAWVDCYTEAGICAVPTNCRTGEGVAEVIAAASA
                     AAKAGAGPDVQRGPSRVRFRTAVLGIPNVGKSSFINRAAKRARVKTGDRPGVTRAKQW
                     IVIDRNLEMLDTPGIMPPRVDDRGVWFALAALGCLDQSIYDVEALATGLIARLDSVGL
                     AAYRSRYGVCEGCVDPHEALKHVARSRGCMRAGGELDMARAADILVRDFRSGKLGRAT
                     LEMP"
BASE COUNT          572 a          598 c          843 g          588 t
ORIGIN      
        1 ggccaagggg tttgcgtctt gtgatcatct ggtaatcatc tgtgggcatt atgagggcgt
       61 cgacgagcgt gtgatgagcc tggtaacgga cagggtgtcc ataggcgact acgtgctcac
      121 tggcggagag ctccctgcca tggtcatcgt tgatgccacg gctaggctac ttcccggcgt
      181 cttgggggac gaggaaagcg cctcggctga ttcgttttgg gatgggattc tcgactatcc
      241 tcattacact cgaccgaggg tttggcgtgg gcatgaagtt cccgacgtgt tgctttccgg
      301 agaccatgag agaataagga aatggcgccg aaaacaggcg cttaaggtta cattggagca
      361 gcgcccggat cttctcagtc aagtcgagct gaccgatgaa gatgtggagt tgctcaagca
      421 agtcatggag gatggctaat aacggttccg gttggcagtc ggtcttcctt gatgagcgca
      481 gttgcattat ggtatactga cgtttgtcga aaacccgttg aggagtggga tagaagtgaa
      541 catcatcgat acaatcgagc gcgagcagat gcgttccgac ctccctgact tcggccctgg
      601 ggatacagtc agagtgcacg ttcgcgttgt ggaaggcgcc cgcgagcgca tacaggtctt
      661 cgagggtgtt gtaataggta gaagcggaag tggcctgtcc gagactttta ctgtgagaaa
      721 aatgtcatct gggatcgggg ttgagaggat ctttccggtt cactccccta agatagacaa
      781 agtagaagta gtcaggcaag gcgatgtgcg taaggcgaaa ctgtattaca tccgcggtcg
      841 cgtcgggaag agggctagga ttcgggagaa gaggcagcac tagtgacaga ctcgccgcgc
      901 ttgacatggc ggctgatttg actagccgtg ggtcgcgcga ggaggttatg aacgcatgag
      961 caattctccc cgaaaaagaa tggaacctcg gttgagccac accgaagctg gcctggatcg
     1021 ggaggcgagc gacgtcgatt cccaaagcca gggcaatgga tccgtgctgg acgaggttct
     1081 cccctgtgaa gggcatgagg agacgcgttc gacgggatcc gtgatcatgg agtatgtcaa
     1141 cgcatgtatc gttgctgtgg ttctggcggc tttcatcatg actttcgttg ccagatcatt
     1201 tgtggttcaa ggaagctcca tggagcctac tttgtataat ggcgagaggc tcctagttaa
     1261 caggttcatc tacagattcc gcgaaccaca gcgtggggac attatcgtat ttcggtatcc
     1321 ccaagacccg cgacagatgt tcatcaagcg tgtcataggt gtccccggag atgagcttat
     1381 gatcagcggg ggcatggttt acgtaaatgg aacaggtttg aatgaaccat atatagccga
     1441 gccttctact agggagtacg gcccggccct tgtcccaacg aagagagtgt tcgtactagg
     1501 ggacaatagg aacaatagtc aagacagcac tgatgattct gtaggcatgg tgccctttgg
     1561 cagcatccaa gggaaggcgt ttgtggtgta ttggccggtg gatcgaatta gaacgcttaa
     1621 gaatccaact tgcctggagg cgattggaac tccagaatcc ggcatggctg cggcgccggg
     1681 catgccttga tggcaatgcg catcgccggg acggatgctc tccgggcccg gcgttatttt
     1741 attttggagg aattgcaatg ggctttagtt ggtatccggg acacatggcc aaggcagtat
     1801 cccagatcaa gcagagcatg aagctgatcg acgtagtcat cgagattctt gatgcccggg
     1861 ctccgagatc tacagaaaac agggagtttg cggaggctgt cggcaacaag cccaggatcg
     1921 tggtgctgaa caaggcggat ctggcagatc cagttgccac tagagcatgg gtcgattgct
     1981 acacggaagc gggaatttgc gccgttccta ccaattgccg aacaggcgaa ggtgtagcgg
     2041 aggtcatcgc cgcggcgtct gccgcggcca aggcgggagc gggcccggac gttcagagag
     2101 gaccttcgcg tgttcgattc cgcacagctg tgttgggcat tcccaatgtg gggaaatctt
     2161 cattcataaa tagggccgct aagcgagcga gggtgaaaac gggtgatcgc cccggggtca
     2221 cccgggctaa gcagtggatt gtgatcgacc gcaatctcga gatgcttgac actcctggga
     2281 tcatgccgcc tcgcgtcgat gataggggtg tttggtttgc cttggccgct ctcggctgtt
     2341 tggatcagtc catatacgat gtggaagccc ttgctacggg acttatagct cggctcgaca
     2401 gcgtgggtct ggccgcgtat cgtagtcgat acggggtgtg tgaggggtgc gtcgatcccc
     2461 atgaggctct gaagcatgtt gcacggagcc gtgggtgcat gagggctgga ggcgagttgg
     2521 acatggcgcg agccgcggat atcctggtga gggatttcag gtcagggaag ctcggtcgtg
     2581 caactttgga aatgccatga c
//
LOCUS       DAOSAI010000345         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_25865_length_2597_cov_3.647915, whole genome shotgun sequence.
ACCESSION   DAOSAI010000345 DAOSAI010000000
VERSION     DAOSAI010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25865_length_2597_cov_3.647915"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLM83_09925"
     CDS             <1..1033
                     /locus_tag="PLM83_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOK71758.1"
                     /translation="ITGGQIPIFLGSSFAFIVVIQTVAEKFGMAYAMGGIVVAGGVYV
                     LVAILVKLIGYDFFKFLFPPVVTGSVITVIGLTLAPTAIQMASSNWPIALAVFAIAAI
                     STLAFKGFMQMLPILIAVVSGYVISLIAGIVDFAPIADAAWFGVPELTFPKFSWEAIA
                     IIAPVAIVTLIEHWGDINANGAVVGKDFVKEPGLWRTILGDGLATMLAGALGGPANTT
                     YSENTATLSLTGVYDPQILRGAAVFAIIIAFCGKLGGIIQSIPEPVMGGISILLFGMI
                     ASVGISTMFSGADMNSTRNRIIAAAVLVFGISKVVVPVGPFEFEGMSLATMTGAILNL
                     VLPQDRPDE"
     gene            1612..>2597
                     /locus_tag="PLM83_09930"
     CDS             1612..>2597
                     /locus_tag="PLM83_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOK71759.1"
                     /translation="MKRVIALILALAALVAVSSPAFGAVTLEVVWMGWPKNLVDKQLE
                     DFRRANPDINLDIQLVPFNQLFQTLEVRLASSRPPDVFIVDGPMTASYAERGYLMPLD
                     SHLSSKELSAWFPASVEACRYRGKLYTLPYATSSVGLYFNKDIFREHNVKLPPEDPGH
                     RLTWEQVVDIARRLTIDTDGDGQPDIWGLIIEQCDRPYQIVPLAESKGGRPLSPDGTI
                     STGYVNSAAFVEAASFYWRLFNDWKVSPKGILQAPIAREYFGNGKAAMMLGAEWNIAQ
                     LRNFPNYEFGLSPHPYFAAGREVTPTGGWHVGISSKTSKKDAALRFAKYITG"
BASE COUNT          599 a          740 c          664 g          594 t
ORIGIN      
        1 gatcacaggt ggccaaatcc ccatcttcct cgggtcttcc ttcgcattca tcgtagtcat
       61 ccagactgta gcggagaaat tcggcatggc ctacgcaatg ggcggcatcg tggtcgccgg
      121 cggcgtgtac gtgctcgtcg cgatcctcgt gaaactgata ggatatgatt tcttcaagtt
      181 cctgttcccc cctgtagtca ctggaagcgt catcaccgtg atcgggctga cactggcccc
      241 tacagcaatc caaatggcca gctcaaattg gcccatcgct ctggcggtat ttgcaatcgc
      301 ggccatctcg acccttgcat tcaagggatt catgcagatg cttccgatac ttattgcggt
      361 cgtgtcaggc tacgtcatct cgctcatcgc aggaatagtc gacttcgccc cgatcgccga
      421 tgcagcatgg tttggcgtgc ccgagctcac attcccgaag tttagctggg aagccattgc
      481 aatcattgca cctgtggcga tcgtaaccct catcgaacac tggggcgaca taaacgctaa
      541 cggtgcagtt gtaggcaaag acttcgtcaa agaacccggc ctatggcgaa ccattctcgg
      601 cgacggtctt gcaaccatgc tcgccggtgc tcttggcgga ccggcgaaca cgacatacag
      661 cgagaacact gccactctga gccttactgg ggtgtacgat ccccagatac ttcgaggcgc
      721 agccgtcttc gccatcatca tcgccttctg cggcaagctc ggagggatca ttcagtccat
      781 acccgagcca gtgatgggcg gcataagcat tctgttgttc gggatgatcg cgtctgtggg
      841 aatctccacc atgttctccg gagccgacat gaactctaca aggaaccgga taatcgccgc
      901 agctgtgctg gtatttggca tatccaaagt agtcgttccc gtcggtccgt tcgagttcga
      961 gggaatgagc ctggctacaa tgactggtgc tattctcaac ctcgtcctcc cccaagacag
     1021 gcccgacgag tagatagctg caacttagac ccaatatgac cggaggcatg tcattcgata
     1081 tggttcggac ggcgcatgca aatggcatcg cgccgtcccc agatattcat acctgaaaga
     1141 aagctggcac agtacaggcc cgagcaatga gtcaccatgt ctctggataa ctcgtgatta
     1201 gtttctgcgc tatatccaaa tcgaccaatc tttgcgaatg gccgatttgc ccatcaatgt
     1261 cttccatgaa aactacccgc actcaaaaca gaacccgtcg cccttgcagt cagcgcacct
     1321 tcggttcctg gtccgggcat aaccgagcta gagcctgaag tagtggcata cattaggcgc
     1381 aagggttttg ggatgcagtt gtcaacagca acatgttgct ttcgaatatt ctaggccaga
     1441 cagggattga cgaggtctga tttctttaga gaaggcgtgc agcttgcccg atctgccata
     1501 agctgtgccg taatcgctaa aaacaggatg gccgaccatt cgcttgcagt ccattccgct
     1561 ttcatgtaca cttgtccatg aacacgctca gctggacagg aggagtaatg attgaaaaga
     1621 gtcattgcgc tcattctcgc gctagcagcg ctggtcgccg tctcatcgcc agcctttgga
     1681 gctgttacct tggaagtcgt atggatggga tggccaaaga atctcgtcga taagcagctt
     1741 gaagatttca ggcgggccaa tcccgacatt aaccttgata ttcagctcgt cccgttcaac
     1801 cagctatttc agacgctcga agtccgcctt gcatcttccc gcccgccaga tgtgttcatc
     1861 gtcgacggac caatgaccgc atcttacgca gagcgaggct acctaatgcc gctcgacagc
     1921 catttgagct ccaaggagct atcggcatgg tttcccgcat ctgtcgaggc atgccgatac
     1981 aggggaaagc tctacactct tccctacgcc acctcttcag tggggctata cttcaacaaa
     2041 gacatcttcc gcgagcataa tgtgaagctc ccccctgaag atccaggcca caggcttacc
     2101 tgggagcaag tagttgatat cgcccgaagg ctgaccatag acaccgatgg cgacgggcag
     2161 cctgatatct ggggacttat tatcgaacaa tgcgacaggc cataccagat cgttccactc
     2221 gcagaatcga aggggggccg cccgctctcg ccagatggga ccatatctac tggctacgtc
     2281 aactctgcgg ccttcgtgga ggctgcgtcc ttctactgga ggctctttaa tgactggaaa
     2341 gtgagcccta aaggaatcct tcaggccccc atcgcccggg agtatttcgg caatggcaaa
     2401 gcagctatga tgctcggggc agagtggaat atagctcaac tgcgcaactt cccgaactac
     2461 gaatttgggc tctcccccca cccttatttt gcagcaggac gtgaagtgac tccaacaggc
     2521 gggtggcatg tgggtatcag ctccaaaaca tcgaagaaag acgcggccct cagatttgca
     2581 aaatacataa ccggtca
//
LOCUS       DAOSAI010000346         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_25893_length_2595_cov_4.643701, whole genome shotgun sequence.
ACCESSION   DAOSAI010000346 DAOSAI010000000
VERSION     DAOSAI010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25893_length_2595_cov_4.643701"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..2059)
                     /locus_tag="PLM83_09935"
     CDS             complement(179..2059)
                     /locus_tag="PLM83_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOK71760.1"
                     /translation="MKFNSLRTKLVVVITCLVLAVVAITGCVFYFQSWSRVFAEIQQR
                     ILAIARTAASNIDGDEHCAVVELRMEDTYAYESIQEYLRRVRNDNPDVVFVYTLAKDT
                     DGEVLIIVDETEGEDHAEIGHLYQSNPAMERAFAGAASVMEDLERDEWGTFLTAYAPI
                     FNCTGDVAGVLAVDLDAGQVLAARKKQISAAVLSAVVGVILAGAIAFALAKSIAVPVK
                     DTVAMLKDISQGEGDLTQRLEVLTTDEIGHLAEHFNVFADKLQGIIGKISITANEVAT
                     ASEELAAGSGEVSKAAQQIAASTVQVAQGAQDESEHARRSVELADRMNESINKLAEDA
                     GQQESHIEETFEVVASMISSLAETGEGLRVTSEASADNARMAGEGIENVNQVIEGIER
                     VKETTDDVVERIEELDAYSQEIGKILEVIGDIADQTNLLALNAAIEAARAGEHGRGFA
                     VVADEVRKLAESSAAETKAIAELVMRVREATAGSVATIRTSAEEVEKVSSLSTETGAV
                     FNRVFEIANRTEKDIENIMGKTDQLLENSARVEEVMRDLVKLAGHNRVVAEELAGLAG
                     EVRESADNTAAVAEETAAAAEESSASVEEISASVEEMTASAESLSRMAGRLTELVGQF
                     KV"
     gene            complement(2236..>2595)
                     /locus_tag="PLM83_09940"
     CDS             complement(2236..>2595)
                     /locus_tag="PLM83_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71761.1"
                     /translation="TLVDILSAAEETVLAIDSMMKEIEQFRMIARQVEANMKEVLEIA
                     QSNTDDAQNMSIHIGKVEQAVETIAAAAAESAASVEEVSASTEEISAAVHRVASSAQT
                     LAEMSARLQQSVAKFTV"
BASE COUNT          550 a          819 c          568 g          658 t
ORIGIN      
        1 actccaaacc aatggtgtca atatagggcg tggtttccaa gcttatactc cacatatcct
       61 atgttgtttc cagtcgcgaa cctccaccga aatagcagtg caggctggaa aaccatcctg
      121 cactgcatgg cctagggcca aatgtccgta atctcgctac ctttcaggcg tgctttcttc
      181 agaccttgaa ctgccccaca agctcggtca atctgccagc catcctcgaa agagactcag
      241 ctgaagccgt catctcttca accgatgcgc tgatttcctc gaccgacgcc gaagattctt
      301 ctgcggctgc agcagtctct tcggcaactg cagcagtatt atctgcagac tcgcgcacct
      361 cacctgccaa gcctgcaagc tcctcggcta cgaccctatt gtggccggcc aatttaacca
      421 gatctctcat aacctcttcc actcgggcac tgttctcgag gagctgatca gttttgccca
      481 ttatgttctc tatgtccttt tccgtccgat tcgcgatctc aaaaaccctg ttgaacacgg
      541 cgcccgtttc tgtggagagg gaacttacct tttccacctc ttccgcactg gtccttatcg
      601 tcgccaccga tccggcggta gcctccctga ccctcatcac caattcagcg atcgccttgg
      661 tctcagcagc cgagctttcg gcaagcttcc tgacttcgtc tgccacgacc gcgaagccgc
      721 gtccgtgttc gcctgctctt gcagcttcta ttgcggcgtt taaagctaag aggttagtct
      781 ggtccgctat gtcgcctatt acttcgagga tcttgccgat ctcctgagag taggcgtcga
      841 gttcttctat tcgctccacc acgtcatcgg tagtctcctt gacgcgctct atgccctcga
      901 tgacctggtt cacattttct atgccttcac cggccattct ggcattatcg gcagaagcct
      961 ctgacgtaac gcgcaatccc tcgcctgtct ctgccagtga tgagatcatg ctcgccacta
     1021 cttcgaatgt ctcctctata tggctctcct gttgcccagc atcctccgcc aacttgttta
     1081 tggactcatt cattctgtca gcaagctcta cgctgcgcct ggcatgctcg ctttcatcct
     1141 gagctccctg ggcaacctgg actgtagatg ctgcaatttg ctgtgccgct ttcgacactt
     1201 cccccgatcc tgccgccagc tcctcgctgg ccgtggcgac ttcgttggcc gtaattgata
     1261 tcttaccgat tatgccctgc agcttgtcgg cgaatacgtt gaagtgctcg gccaaatggc
     1321 cgatttcatc cgttgtcaat acctcaagac gttgtgtgag gtctccctcc ccctgggaaa
     1381 tgtctttcaa cattgctacc gtgtccttca caggcacagc aatgcttttt gccagtgcaa
     1441 aggctatggc tccggcaaga attactccaa caacagcact caaaaccgcc gccgatatct
     1501 gcttcttgcg tgccgccagc acttggccgg cgtccagatc gacagcaagc acgccggcga
     1561 catcacctgt gcagttgaag ataggagcat aggctgtaag aaaggtaccc cactcgtctc
     1621 tctcaagatc ctccatcaca gaggcggcgc cggcaaaagc ccgctccata gcggggttag
     1681 attgatacag gtgcccaatc tccgcgtggt cctcaccctc agtctcgtca acaattatga
     1741 gtacctcgcc atccgtatcc ttcgccagag tgtacacgaa aaccacgtcc ggattatcgt
     1801 ttcgcactct cctcaggtac tcctgaatcg actcatacgc ataggtatct tccattcgaa
     1861 gctcaacaac ggcacagtgt tcgtcgccat ctatgttcga agccgcggtc cgggctatgg
     1921 ccagtatgcg ctgctgtatc tcggcaaaca cccgtgacca gctttgaaaa tagaatacac
     1981 atcccgtgat agccacaaca gccaaaacca agcaggtaat cacaacaacc agcttggtcc
     2041 tgagtgaatt gaatttcact gtatctaccc ccaaaaaaca tccctgaagg cctttcgacg
     2101 cactggccta caattcccga aatcatatat tccatcgggc atacccgcca accaggcatc
     2161 aattgccgca gcagatcaag accgcctttt gcgacacaaa ttcccctgcg gtaaaggaca
     2221 tgcgggttgc gccgactaca ccgtgaattt agcaacgctt tgctgcaggc gggcggacat
     2281 ttcggcaagg gtttgggcag aagacgccac ccgatgtaca gctgcgctga tctcctcagt
     2341 cgaagccgag acttcttcta ccgatgccgc gctctctgca gctgcagccg caatcgtttc
     2401 tactgcctgt tcgactttgc cgatgtgaat actcatgttc tgtgcatcat cggtattgga
     2461 ctgagcaatc tcgagtacct ccttcatgtt cgcttccacc tgcctagcaa tcatcctgaa
     2521 ctgttcaatc tctttcatca tgctatcaat ggccaagaca gtctcttcgg cggcgctcaa
     2581 tatgtcaaca agcgt
//
LOCUS       DAOSAI010000347         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_26082_length_2580_cov_3.329109, whole genome shotgun sequence.
ACCESSION   DAOSAI010000347 DAOSAI010000000
VERSION     DAOSAI010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26082_length_2580_cov_3.329109"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /locus_tag="PLM83_09945"
     CDS             <1..1808
                     /locus_tag="PLM83_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOK71762.1"
                     /translation="LRGQVFDEFFLMSIASLGAIAIGELPEAVGVMAFFAVGELLQDR
                     AVERSRTSIRALMDVRPDYANLVRDGECIRVSPEEVSTGDLIMVRPGERVPLDGVIID
                     GETTMDTSALTGESMPRTFGAGDQALAGMINRSAAVTVRTLRPYSDSSAARILELVEE
                     AETRKAHTERFITTFSSYYTPAVVGLAVAIAVLPPLIIPGQVFSEWLHRALVLLVVSC
                     PCALVISIPLSYFAGIGGASRRGVLIKGADCLEALNDVDSVIFDKTGTLTQGSFRVVE
                     TTGENGFTGYEVLRLAAHAGANSSHPVSTSIREAFEGEPGRGRIDVSVISDSRELAGM
                     GVAAEIGGERVIAGNDRLLHHENVVHDVCVTDGTVVNVAKDGILAGRIRLADEIKPGA
                     SEAVQRLHEAGVNRIVMLTGDVGDIAERVAGEVGIGEYRANLLPDEKVRAVEMIMAEQ
                     VSMAQSGGGWPDASAHRRACGASGRGAGKVAFVGDGINDAPALARADVGIAMGALGSD
                     VAIEAADVVIMDDNPARIADAIETARRTRRIVMENIVFAIIVKLAVLAMGTMGVATMW
                     NAVFADVGVALIAVVNATRAYGNLGHARGSASGRA"
     gene            complement(1872..>2580)
                     /locus_tag="PLM83_09950"
     CDS             complement(1872..>2580)
                     /locus_tag="PLM83_09950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOK71763.1"
                     /translation="SFVSRTSSAARAAPLLSMTVTKPMDGRKRAILTFDDGPSSTYTP
                     EILDILKDEGVHAVFFVVGSQAESRPHLLSRMAAEGHEIGNHTYSHLDPASATYEELV
                     CEIRRTSEIVEQATGSPTTWLRPPGGVVSPEVISAAESTGHNIVLWNVDTRDWRVAKQ
                     ADADRLVAEAVASATASQVSIVLMHDGGGQRNATVSALRSIIQLLRDNGYEICSLAEL
                     VGTTDDGKASPSPIWTR"
BASE COUNT          532 a          640 c          837 g          571 t
ORIGIN      
        1 tactccgtgg gcaggtgttc gacgagttct tcttaatgag catcgcatct ctcggtgcta
       61 ttgcgatagg agaattgccc gaggccgttg gagtgatggc tttcttcgcc gttggcgagc
      121 tcttgcagga cagggctgtg gagcgttcac gcacttccat aagggcgctg atggatgtaa
      181 ggcctgatta tgccaatctt gtgcgtgatg gggagtgcat acgggtcagc ccggaggaag
      241 tgtctacggg cgatctgatc atggttaggc ctggcgagag ggttccattg gacggcgtga
      301 ttatcgacgg cgagactaca atggatacat ctgctctgac cggcgaatcg atgcctcgga
      361 ccttcggcgc cggggatcag gcattggcgg ggatgatcaa cagaagcgct gctgtaaccg
      421 tcagaacctt gaggccctat tcggattcgt cggcggctcg cattcttgag ctggtggaag
      481 aggccgaaac tcggaaggcc cacacagagc ggttcataac aacattcagc agctactata
      541 ctccggcagt tgttgggctg gccgttgcga ttgcggtctt gcctccgctg atcatacctg
      601 gtcaggtttt ctccgaatgg ctacacaggg ctctggttct tcttgtggta tcatgcccgt
      661 gtgcactcgt gatctcgatc cccctcagtt attttgcagg cataggggga gcgtcgaggc
      721 gcggcgtgtt gataaagggc gcagactgtt tggaggccct aaacgatgtg gactcagtga
      781 tctttgacaa gactggcaca ctcactcaag gctcatttcg ggttgtggag acaacaggcg
      841 aaaacggttt tacagggtac gaggtgctca ggctggcggc gcacgccggc gccaattcaa
      901 gccacccggt atcgacatcc atccgtgaag cattcgaagg tgagcccggt cggggcagga
      961 tagatgtctc agtaatctcc gacagccgcg aactggccgg aatgggagtt gcagctgaga
     1021 tcggagggga gcgggtgatt gccgggaacg accgcctgtt gcatcatgaa aacgtggtcc
     1081 atgatgtatg cgttactgac gggactgtgg tgaacgtggc caaggacggg atcctcgcag
     1141 gcagaatccg tcttgcagat gagataaagc caggggcgag tgaggcagtg cagcgccttc
     1201 atgaagcagg cgtcaacagg atagtcatgc tcactggcga cgttggcgat atcgcggagc
     1261 gcgtggccgg cgaagttggg ataggcgaat atcgagcgaa ccttctgcct gatgagaagg
     1321 tcagggcggt agagatgatc atggctgagc aggtgagcat ggcacaatcg gggggaggct
     1381 ggccagatgc gtctgcacac agaagggctt gcggcgcttc gggaagggga gccggaaagg
     1441 ttgcttttgt gggcgatggg ataaacgatg ctccagccct tgccagagcc gacgtgggca
     1501 ttgcaatggg ggcgttggga tctgacgtgg caattgaggc agctgacgtg gtgatcatgg
     1561 acgacaatcc tgcccgaatt gcagatgcca tcgaaactgc gcggcgcacc aggcggatag
     1621 tgatggagaa catcgttttc gccataattg tgaagctggc ggttcttgca atgggcacca
     1681 tgggtgtggc aaccatgtgg aacgccgtct ttgccgatgt aggtgttgcg ttaatcgcag
     1741 tcgtcaatgc gactagagca tatgggaatc tggggcatgc gcggggatct gcgagcggcc
     1801 gggcctgagg cgctaatggc gccgtgggct ggcagactgg caggggagac gcacatgccc
     1861 gctctctggt ttcatcttgt ccatattggc gacggtgagg ctttaccatc gtcagtcgtg
     1921 ccgactagct ccgcgaggga gcatatctcg tagccgttat ccctcaaaag ctgaatgatt
     1981 gaccttagcg cggagactgt ggcgtttctc tggcctccgc catcgtgcat gagcactatg
     2041 ctcacctgcg aagccgtcgc gctggcaaca gcttccgcca ccagccgatc tgcatctgct
     2101 tgtttggcga cgcgccagtc ccgggtatcc acattccaca ggactatatt gtgtccggtc
     2161 gattctgcag cggaaatcac ttccggtgat acgactccgc caggtggcct caaccaagtt
     2221 gttggtgagc ccgtggcctg ctcgactatt tcagaagtgc gccgtatttc gcagacaagt
     2281 tcttcatatg tagcagatgc gggatcgaga tggctgtaag tgtgattgcc gatctcatgt
     2341 ccttcagcgg ccatccttga gagcagatgg ggacgggatt ctgcctgact gccaacgacg
     2401 aaaaagactg cgtggacacc ttcgtccttc agaatatcga ggatctcagg ggtgtaggtt
     2461 gaagagggcc cgtcatcgaa tgtgaggatt gcgcgcttcc gaccgtccat cggcttggtc
     2521 acggtcatgc tcagcaacgg cgccgcacgt gcagcactag atgtcctgga aacaaaggaa
//
LOCUS       DAOSAI010000348         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_26176_length_2574_cov_4.456133, whole genome shotgun sequence.
ACCESSION   DAOSAI010000348 DAOSAI010000000
VERSION     DAOSAI010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26176_length_2574_cov_4.456133"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLM83_09955"
     CDS             <1..505
                     /locus_tag="PLM83_09955"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOK71764.1"
                     /translation="FHDIGKIGIPDSILQKPGPLTDEEWKVMKAHPEIGARIVGGSYE
                     LSHISESILSHHERWDGEGYPEGVGGDEIPLISRILAIVDAYDAMTSDRTYRKAMSRE
                     EALAEIARCSGTQFDPDLVEMFLTMMEEMARSEEDEMDWLTAGMQWYEGLGDCDSGVP
                     KMPEDEA"
     gene            complement(561..2297)
                     /locus_tag="PLM83_09960"
     CDS             complement(561..2297)
                     /locus_tag="PLM83_09960"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOK71765.1"
                     /translation="MQRSAAERVYIIGHKNPDCDSIVSAVAYAEIKNALAGWDSPHAS
                     THTTEEPETPASCGETFPSKYVPARSGPVNHETATVLETFGFESPQFLPNIHPQVSDV
                     QFDSPMLVRTGSPLRKVWKQMLETRAATACIVDEDGKLQGLATLGDIARAELNASAKR
                     SHYSFPIKNLVSALDAQLVAEGQPEFDGRVFVGDIGIDWARRAITPHDMLIVSDRPDL
                     QRIAVEEGVAVLVVTDAHRAPVSRDTLELARSRGTSVLSTAADTLACLGLLWQAVPVD
                     QVMTSADVATFTLDDLVSEVREEMRLRPFKLYPVMDEHGRPTGMIGRNHLADPPRKRV
                     ILVDHNEKTQSVEGIDTAVVLEVIDHHRIGSIETDHPILFVNRPWGSTSTIISALARQ
                     NKVTLSPALAGLLASAIISDTLAFKSPTSTPQDRLEAERLASGADVDLKSLADLVLTA
                     KTHIEDADPAEILRSDFKEFTVGKFKVGIGQATTSGGFSSDLKRQLLDATRSLMEDAG
                     YAMVALMLTDVAADGSEILWTARDASLAERAFGHAGVEAGAESFWLPGVVSRKQQFAP
                     EIMRALRELERS"
BASE COUNT          482 a          681 c          788 g          623 t
ORIGIN      
        1 tttccacgac atagggaaga tcgggatacc cgacagtatc ttgcagaagc ccgggcctct
       61 cactgatgaa gaatggaagg tgatgaaggc tcatccagag ataggcgcca ggattgtggg
      121 gggatcatac gagctttcgc atatttcgga atccattctc tcccatcacg agaggtggga
      181 tggggagggg taccctgagg gggttggcgg agacgagata ccgcttatct cccgaatcct
      241 cgcgattgtc gatgcgtatg acgcgatgac cagtgatcgg acatacagaa aagccatgtc
      301 gagggaggaa gctttggccg agatagcgcg ttgctccgga actcagttcg atcccgactt
      361 ggtggagatg ttcctcacta tgatggagga gatggccagg tcggaggagg acgagatgga
      421 ctggttgact gcggggatgc agtggtacga aggtttgggc gactgtgatt ccggcgtgcc
      481 caagatgccc gaagatgaag cgtagtgggc atcacggcgt ccgggccatg ggcccggaca
      541 tgccttttca gcccggtgtg ctaactccgc tccagctccc tgagtgctct catgatctcc
      601 ggtgcgaact gctgttttct tgacactacg cccggcagcc agaacgattc tgctcccgct
      661 tcgaccccgg cgtggccgaa ggcgcgctct gccaggcttg cgtcacgcgc cgtccacagt
      721 atttctgaac cgtctgccgc cacatctgtg agcatcagcg ctaccatagc gtagcctgca
      781 tcttccataa ggcttcgcgt ggcgtctaga agttggcgct tgaggtcgga gctgaaccct
      841 ccggaagtgg tagcctgccc tatccccact ttgaacttgc ccacagtgaa ctccttgaag
      901 tcggatcgca gtatttcggc gggatcggcg tcttctatat gggtcttcgc cgtgagcacc
      961 agatcggcga ggctcttcag gtcgacgtcg gctcctgagg ccaggcgttc cgcttccagc
     1021 ctgtcttggg gtgtagatgt gggcgacttg aaggccaggg tgtcggagat gattgcggat
     1081 gccaatagtc ctgccagggc agggctgagg gtgactttgt tctgccgcgc caaggcgctt
     1141 attatggtcg aggtagatcc ccatggcctg ttcacaaaga gtatgggatg atctgtctca
     1201 atcgacccta ttcgatggtg gtcgatcact tccaacacta cggccgtgtc gatcccttcc
     1261 actgattggg ttttctcgtt gtgatccacc aagatcactc gcttgcgggg agggtcggcc
     1321 agatggtttc tgccgatcat gcctgtgggt cttccgtgct cgtccatgac cggatagagc
     1381 ttgaacgggc gaagcctcat ttcctctctc acttctgaca ccaggtcatc aagggtgaat
     1441 gtggccacgt cggcgcttgt catcacttgg tctacgggga ctgcctgcca cagaagcccg
     1501 aggcaagcga gggtgtctgc tgccgttgac agcacggatg tgccgcgcga gcgcgccagt
     1561 tcgagcgtgt cgcgggagac gggggcccga tgggcatctg tcactacgag cacagcgacc
     1621 ccctcttcga ctgcgattcg ctggaggtcg ggcctatcgg aaacgatcag catgtcgtgg
     1681 ggggtgatgg cgcgacgggc ccaatctatt ccgatgtcgc ccacgaatac tcggccatcg
     1741 aactcgggct ggccttctgc tacgagttgg gcgtcgaggg cgctcacgag gttttttatg
     1801 gggaacgaat agtggcttct ctttgctgag gcgttaagct cggccctggc gatatcgccc
     1861 aaggtggcaa ggccctgaag cttcccgtcc tcgtccacaa tgcatgccgt ggctgccctc
     1921 gtctcgagca tttgcttcca tacttttcgc aagggagagc ccgtacgcac cagcataggg
     1981 ctgtcgaact gcacgtctga cacctgcgga tggatattgg gcaagaactg gggcgattcg
     2041 aatccgaatg tctcgagcac ggtggctgtt tcatggttga caggcccaga ccgcgcaggc
     2101 acgtactttg aaggaaaagt ttccccgcag ctcgccggtg tttcaggttc ctctgttgtg
     2161 tgggtgctcg cgtgggggct atcccaacct gccagcgcgt tcttgatttc cgcataggct
     2221 actgccgaca ctatcgaatc gcagtcggga tttttgtggc caataatgta gactctttcc
     2281 gcggctgatc tttgcatgtg aatatcacct atgtttcatg gaattctata gacaatttcc
     2341 ggcgtgtttc gttttttctg cgtctgtcct tgattgagag ggcccgatcg cttcttcgaa
     2401 gctgtttttc aggaggctca ctgagatttg ctgctcagac cgggtaaccc gcctgcgatg
     2461 gaatcgcctt gatacacgta ttttacatga tttttgcggc agtgtttatc acttttcaga
     2521 tggagtgtgt gataagtagg caagagtgca gttgggagtc ggggggatgg tcga
//
LOCUS       DAOSAI010000349         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_26209_length_2572_cov_3.176003, whole genome shotgun sequence.
ACCESSION   DAOSAI010000349 DAOSAI010000000
VERSION     DAOSAI010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26209_length_2572_cov_3.176003"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PLM83_09965"
     CDS             complement(<1..1563)
                     /locus_tag="PLM83_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOK71766.1"
                     /translation="MRILDRYVVHEFMGPFILSILAFIVIMLSGQLFWLVDLIVQKKV
                     PVLIVARMLLYSLPGIVVQVLPVAVLFGVLIGLGRLARDSELQIMRVSGIPFARIALP
                     LIVLGAVMSAVGFWLNESVAPWATHQSEIIVREMIFQEAFPDVEEDVFFKAPGNRYFY
                     VGKADQKRGTFERVMIYETARGEFPRLVSARSGRAESGKWVLEDGSIHELDEEGHVRM
                     AISFETLEISLEQSLDSFASSQKTPEEMSMKELKSNIDLFERSGIRVDALRVDYYLKV
                     AHHVASLVFAVLGAPLVVKSPRRGGGYFGVVAAAVLSFAYYIVQALARSVAMRGGLSP
                     LLAAWLPNIVFGVPGLFMLFAVDRPRMNLAARRRNSGGGDGSAGIKFSRGGVHMWLGV
                     SILTTLYIFAICSPPAVASNIPVKVDAEKLVYDGAIGLWVMSGNVKIEYKDLVISGDY
                     AEFNMDRKVVAVNGSVRVVSGQDALAGESAIFNVSTGEVVVDEAWAKLTGEGVEGFLY
                     MSGSQLQKEDDYL"
     gene            complement(1761..2219)
                     /locus_tag="PLM83_09970"
     CDS             complement(1761..2219)
                     /locus_tag="PLM83_09970"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOK71767.1"
                     /translation="MFKNVPKTLLIVLVCAILVGAVAVVANLSAVSRAAGEAAVIGKV
                     DGVRLGQEFPAMQKAVDNILKQKAALEKELNTKAEGQDQETQRTLLDQYNLKYNEYAK
                     QQLEPAENALKSAIAAVAKEKGVTVVLDSSVVHLGGIDMTDAVLKKGGAK"
     gene            complement(2327..>2572)
                     /locus_tag="PLM83_09975"
     CDS             complement(2327..>2572)
                     /locus_tag="PLM83_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOK71768.1"
                     /translation="GDRMALANAEYRFNITKGLQGVLFCDAGQAWNADGSLDVGSTKV
                     GYGAGVRIMVPVLGMIRVDYGIGEKGGQAYFSFGQTF"
BASE COUNT          677 a          807 c          562 g          526 t
ORIGIN      
        1 aagataatca tcttcctttt gcaactggct tccagacatg tacaggaatc cttcaacgcc
       61 ttcgcctgtc agcttagccc aggcctcatc aaccacaacc tcgcccgtgg aaacgttgaa
      121 aatggcgctt tcgcccgcga gcgcatcctg ccctgaaacg accctgaccg acccgtttac
      181 tgccacaact ttccgatcca tattgaactc agcgtaatct ccggatatca cgagatcctt
      241 gtactcgatc ttcacattcc ccgacatcac ccaaagacct attgcgccgt cgtacacaag
      301 tttttcggcg tctaccttga caggaatatt gcttgcgacc gcaggagggg agcagattgc
      361 gaaaatgtac agagtcgtca agatcgatac cccaagccac atatgaacgc cgcctctgga
      421 aaacttgatc ccagcagacc catcgccccc tcccgaattc ctccttcttg cagccaaatt
      481 cattcgggga cggtcaactg cgaacaacat gaaaagacct ggcactccaa agacaatatt
      541 gggcaaccaa gcagctaaaa gcggggacag gccgcccctc atggccacag atcgcgccag
      601 agcctgcacg atgtaatagg caaaggagag aacggctgcc gccacgaccc cgaagtaacc
      661 gccgccacgt cggggagact tcacgacaag cggggcaccg agaacggcaa agaccaaaga
      721 tgccacatga tgggcaacct tgagatagta atcgacgcgg agcgcatcta cacggatccc
      781 gctcctctca aaaaggtcga tattggactt gagttccttc atgctcattt cctctggggt
      841 cttctggctc gatgcgaagc tatcgagcga ctgctccagg gaaatctcca gagtctcaaa
      901 ggaaatcgcc attcgcacat gcccttcctc atcgagctca tggatactgc catcctcaag
      961 cacccacttg cctgactcag cccgaccact cctggcagag acaaggcgcg gaaactcgcc
     1021 cctagccgtc tcgtatatca tcacacgttc aaacgtgcca cgtttttggt cagccttgcc
     1081 cacgtagaaa tagcgattgc ccggcgcctt gaagaaaaca tcctcttcga catcgggaaa
     1141 agcctcctga aatatcatct ccctgacgat aatctcggac tgatgagttg cccacggcgc
     1201 cacgctctca ttgagccaaa atccgactgc gctcataacc gcccccaaaa cgatgagagg
     1261 caaagcaatc ctggcgaaag gaatcccgga aacccgcata atctgaagtt cagaatcgcg
     1321 agccagcctg ccaaggccga tcaagacgcc aaacagaacc gcaacaggca aaacctgaac
     1381 cacgatcccc ggaaggctgt aaaggagcat cctagcaaca atgagcacag gcactttctt
     1441 ctggactatc aggtctacaa gccagaagag ttgcccactc agcattatca cgatgaacgc
     1501 caatatagat agaataaagg gacccatgaa ctcgtggact acatacctgt caagaatcct
     1561 catcagtcgt gacctcgcag tctacagctg cctcttgagc gcaactgagc atcgcaatcg
     1621 catctttggc acaaccccat gcatttcaac ataacagccc tgcccccgcg gtgggcatag
     1681 agcgtgccaa agcgcctcaa gacaagaagg gggagacgaa tgtcgcctcc ccaagtgtga
     1741 tgcactgccg ctacttgcta ttatttcgcc ccgccttttt tgaggacggc gtctgtcatg
     1801 tctattccac caaggtggac aacgctcgag tccagaacga cggtaacgcc cttctccttg
     1861 gcgactgccg ctatggcgct cttcagagca ttctccgcag gttccagctg ctgctttgca
     1921 tactcattgt acttcagatt atactggtca agcaaagtcc tctgggtctc ctgatcctgc
     1981 ccctcagcct tggtgttcag ttccttttcc aaggcagcct tctgcttgag aatgttgtca
     2041 acagctttct gcatcgcagg gaattcctgt ccaagcctga ccccatcaac ttttcctatg
     2101 acagcggcct cgccagcggc ccgcgataca gccgaaagat tagccacaac agcaacagca
     2161 ccgaccaaaa tggcacacac caaaacaatc aggagagtct tcggcacgtt tttgaacatt
     2221 aatgtcatcc tttccaatca actcaaaaat cgcaatcaga acagtataac gaggagcgcc
     2281 gacgacgcgg cgccccgtca attatactac atgagccagc ttaagactag aaagtctgcc
     2341 caaagctgaa gtaggcctgt cctccctttt caccgatgcc ataatcgaca cggatcatgc
     2401 ccagcaccgg aaccatgatt ctcacgccag ctccataacc gaccttagta cttccaacgt
     2461 ctaaagagcc atccgcgttc cacgcctgcc ccgcatcgca aaacagcaca ccctgcagac
     2521 ctttggtaat gttgaaacgg tattccgcgt ttgccagggc catgcgatca cc
//
LOCUS       DAOSAI010000350         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_26436_length_2556_cov_3.157537, whole genome shotgun sequence.
ACCESSION   DAOSAI010000350 DAOSAI010000000
VERSION     DAOSAI010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26436_length_2556_cov_3.157537"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /locus_tag="PLM83_09980"
     CDS             complement(<1..1963)
                     /locus_tag="PLM83_09980"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOK71769.1"
                     /translation="MQLLAHTIVKNRHLVLVIALILLIPSVYGVLHARIEYDLMSYLP
                     DDLDSVRGMDILDREFHYADRAIVVIRDDPDWKVLKLKEMIEQVDGVSSVFWISDISD
                     PAIPHDYLGEEIVSQFYKGDITFLLVHLAEGGISLESEKAIADIKALLDDNQRLIGQV
                     VSGIEMKDLGMSQTPGMVRAAVILIFLVLLLALPSVALPFIFMATIGAAYAYNMGIAY
                     IFGQRVSYITNSCAAALQLGVTMDYCIFLIHSFQAERKTHDPETAMEKAIASTATAVV
                     ASAITTATGFAALALMSVRLGADMGLFMARGILLSVIATLTVLPSLVLVFQSVIDKFA
                     HRSFLPRFDGIANWAIRRRVPVFALAVVIMAAGYYGYSKVELNYDLEATFPQEIPSIV
                     AMEEFSEAFGSMREAYVVIRDMPGWRLQQITAELREIEGVNSAECLADLVDPAIPLDF
                     VPGEVAKRYVGGGYSNIILNIAGSGLDDTTENLIANVREVLADVDEECYLTGSPVITS
                     DLRRITDRDMVVVNAVSIVAIFVIVMIAFRSPTVAIVLVAAIMSAIWANQAISGILGQ
                     PLFFFSGLAIGAIQLGATVDYAILVASTFREKLGELDPVEGMRRTMIECGPAILNSGL
                     ALFAAIMGVYSATTLLAVKELGLLLARGAL"
     gene            2360..>2556
                     /locus_tag="PLM83_09985"
     CDS             2360..>2556
                     /locus_tag="PLM83_09985"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640696.1"
                     /note="regulates pyrimidine biosynthesis by binding to the
                     mRNA of the pyr genes, also has been shown to have uracil
                     phosphoribosyltransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase"
                     /protein_id="HOK71770.1"
                     /translation="MQLRDKNVIMDEMDMKRALTRISHEIIEANKGIESVALVGIRRR
                     GCPLAYRLADQIEAIEGAKPPV"
BASE COUNT          638 a          729 c          629 g          560 t
ORIGIN      
        1 tcagagcccc tctggccaga agaagcccca attccttaac tgccagcagt gtagttgcag
       61 agtagacccc cattatcgca gcgaacagcg caagaccgct gttgagaatc gctggcccgc
      121 attctatcat ggtgcgccgc atcccttcta cagggtccaa ctcgcccagt ttctcccgga
      181 acgtgctggc cacgagaatc gcgtagtcca ccgtggcacc tagttggatc gcacctatgg
      241 caaggcccga gaagaaaaag agcggctggc ctaagatccc tgatattgct tggttagccc
      301 atatcgcgct cattatggcg gcgacaagga caatcgctac ggtgggagaa cggaatgcga
      361 tcatcacgat gacgaagatc gcaactattg agactgcgtt aaccactacc atgtcccggt
      421 cggtgatcct ccgaagatca ctggttatga caggacttcc ggtaagatag cattcttcat
      481 ccacatcagc caatacttcc ctgacatttg caatgaggtt ttcagttgta tcatccaggc
      541 ctgagcctgc tatgttcagg atgatgttgc tgtacccacc acccacatat ctcttggcca
      601 cttccccagg cacaaagtcg agagggatcg ccggatccac cagatcggcg aggcattcag
      661 cagaattcac gccttctatc tcccgaagct cagccgttat ctgctgcagc cgccagccag
      721 gcatatcgcg aatgactaca taggcctccc gcatgctccc aaaagcctca ctgaactcct
      781 ccatggcgac gattgacggt atctcctggg gaaaggtcgc ctccaagtcg taattgagct
      841 ctaccttgga gtacccataa tagcccgcgg ccatgattac aacagccagg gcaaaaacag
      901 gcacccttcg ccggatcgcc caatttgcga tcccgtcaaa tctgggaaga aacgatcggt
      961 gggcaaactt gtcgatgaca ctttgaaaga ccagtaccag actgggaagt accgtcaatg
     1021 tagcgattac ggagaggagt atgccccttg ccatgaaaag ccccatatcc gccccgagcc
     1081 tcacgctcat cagcgcgagc gccgcaaatc cggtggcagt tgtaatggca cttgcaacga
     1141 ccgccgttgc agtgctggca atcgccttct ccatcgctgt ctccgggtcg tgcgttttgc
     1201 gttcagcctg gaaactgtgg atgaggaaaa tgcagtagtc catcgtcacg ccaagctgca
     1261 acgcagcagc gcatgagttg gttatgtatg acacccgttg tccgaagatg tacgcaattc
     1321 ccatgttgta ggcataggcc gcccctatag tcgccatgaa tatgaagggc aatgctaccg
     1381 aaggcaaagc aagcaaaagc acgaggaata tcaagatgac cgccgcccga accattccag
     1441 gcgtttggct catccccaga tccttcattt caatgccact tacaacctgg cctatgagac
     1501 gctgattatc gtctaacagg gccttgatgt cggctatcgc tttttcggat tcaagcgata
     1561 tgccgccctc cgccagatgc accaacagaa atgtgatgtc gcccttatag aactgggaga
     1621 cgatttcttc tccaagatag tcgtggggga tcgctgggtc cgaaatgtcc gaaatccaaa
     1681 agacggacga gaccccatcg acctgttcga tcatctcctt cagcttgagg actttccaat
     1741 cgggatcatc cctgatgaca actattgcac gatcagcata atgaaactcc ctatccagaa
     1801 tatccattcc ccgtacggaa tcgaggtcgt ccggaaggta tgacatgaga tcatattcta
     1861 ttctggcatg aagcacgcca tatactgacg gaatcaacaa gatcaaggct ataaccaaga
     1921 caagatgcct gtttttcacg atcgtgtgag ccaatagttg cataccagat ctccatccac
     1981 aaattattgc actgaccagt cagtgcagta atatggtagc gctatcccct tcccctgtca
     2041 acaggcccaa ttggctccac cagcccaaca ggactcatat ccagctttgc agttgaccac
     2101 tgctgcggcc tgcaccgtac gtgcctagca ctgaatgcgc tgccaagcgc cttgggtact
     2161 gcacttgagc gggatgcaag agaatgtgcg tgtggccatt gactttggga ccctattgcc
     2221 ttaaaatcaa atgcgactga gcagaacctt taagggtagc cccgtgaggc taacaaggat
     2281 cgctgcacgc atgctgttcc tgcctggcct cacggcttgt gcaggttctt tttatgccca
     2341 caattaagga gggtggccca tgcagcttcg agacaagaat gtgatcatgg acgaaatgga
     2401 catgaagcgc gcgctcactc gcatctccca tgagataata gaggccaata agggaatcga
     2461 gagcgttgcc cttgttggca tcaggcggag gggatgcccg ctcgcttaca ggctcgccga
     2521 tcagatcgaa gccatcgaag gcgcaaagcc ccctgt
//
LOCUS       DAOSAI010000351         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_26504_length_2551_cov_3.789663, whole genome shotgun sequence.
ACCESSION   DAOSAI010000351 DAOSAI010000000
VERSION     DAOSAI010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26504_length_2551_cov_3.789663"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            442..705
                     /gene="rpsO"
                     /locus_tag="PLM83_09990"
     CDS             442..705
                     /gene="rpsO"
                     /locus_tag="PLM83_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOK71771.1"
                     /translation="MDKETKGHIIDEFRIHAADTGSPEVQVAILTYRINQLTEHLKVH
                     GKDHHSRRGLLKMVGKRRKLLSYLREKDFGRYRDLIQRLGLRH"
     gene            923..>2551
                     /locus_tag="PLM83_09995"
     CDS             923..>2551
                     /locus_tag="PLM83_09995"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOK71772.1"
                     /translation="MTDVFRIAVGDRDIIFEYGHLAKQANAAVKVSCGDTVLLVTTCM
                     SEEPREGLDFFPLLVDYEERFYAAGKIPGGFIKREGRPSEGAILNARLIDRSIRSLFP
                     EDLRNDVHVVAMVLSVDQQNAPSIPAINGASAALTISDVPWNGPVGAVRIGRIDGHFV
                     VNPTEEEMANSELDLVVAGHADGITMVEAGAKEIPEDMMIEALDLAQAEIRKITTELV
                     RIRERLGKPKAEIPSLPSVPEIDEWIAEECLEDIRKAVRINEKKERNTAIGDVIKRAL
                     ERFLESYPDSAAYVASTVEGHVKRELRAMIVEEKRRPDGRAMSELRPISCEVGVLPRT
                     HGSALFTRGQTQALVVTTLGMVGEDEQILDSLKLDEPPKHFMLHYNFPPFSVGEVRPM
                     RGPGRREIGHGALAERAIAPLVPAEGDFPYIVRVVSDILESNGSSSMASVCGASMSLM
                     DAGVPLKKACAGVAMGLIKEGDKVEILTDIQGLEDHYGDMDFKVAGTRDGITALQMDN
                     KAGGIDRGILERALSQAKEARLAILDIMESAIPSP"
BASE COUNT          657 a          584 c          752 g          558 t
ORIGIN      
        1 atgggttcat cgtgagtata aaatccctgc taccggaact ccaagaactc atacgttaag
       61 aagaaagcca taaaaaacaa aaatgaatat aaaatagtgg gattttttgt ttgtccataa
      121 cagatcagag gacaagcaag taattgtatt attcaataaa aaataagggc gccttcagga
      181 tattaggcat cgaggattat tggtatcaat atcgagataa ggcaataaaa caaattgcga
      241 tagattggtg taataagaat ggaatagctt tcgcaatcag cagcaataat taaacatggc
      301 tttggataat agaaggggtt agcaacttgg tgcgggttgt tgtaatgtgc gcggcatacg
      361 tgttagaata aatgcaccct tgctcggagc gggctttgcc ctcccttggc aacgggattt
      421 cgtggttatg aggtgagatg ggtggacaaa gaaacaaaag ggcacatcat tgacgagttc
      481 cgtatccacg ctgcggatac agggtctccc gaggtgcagg tggcgatctt gacgtatcgc
      541 ataaatcaac taaccgaaca cttgaaagtt cacggcaaag atcaccactc tcgaagggga
      601 cttctcaaaa tggtgggcaa gcggcgcaag ttgctgagct atctgagaga gaaggacttc
      661 ggtcgataca gagatctcat tcagcgcctc ggtttgaggc attgaggatc ttctatacag
      721 ggcagtttct gcgcgatgga ggcgagggcg gtgctcttgc ctctgttttt cctgttagga
      781 ttgtgcggtt ggttaaaatg tcgtgcccgg tgtggcgcga tggtataggg gtgtgaaaga
      841 gaggcatcga aactccaaag aggagcttac gacatgcttt tgctaaccat aagaaggcga
      901 aagaggagga agagatagaa gaatgacaga tgtttttcgg attgccgtgg gtgatcgcga
      961 tattatcttt gagtatggtc acttagccaa acaggccaat gcagccgtca aggtttcatg
     1021 cggcgataca gtgcttttgg ttacgacctg tatgtcagag gaaccgcggg aggggttaga
     1081 ctttttcccc ttattggttg attacgaaga gcgtttttat gcggccggca aaatacctgg
     1141 cggttttatc aagagggagg ggcgcccgtc tgagggggca atactcaacg cccgcctcat
     1201 cgaccgctcg atccgctcgc ttttccctga ggatttgcgc aacgatgtcc atgtggtggc
     1261 catggtgctc tccgtggacc agcagaatgc ccccagcata cccgcaataa acggcgcatc
     1321 tgccgctttg accatttccg atgttccgtg gaacgggccg gtaggggctg tgcgcatcgg
     1381 tcgcatcgat gggcattttg tcgtgaaccc cacggaggaa gagatggcca acagcgagtt
     1441 ggatctagtc gtggctggcc atgccgacgg catcacgatg gtggaggccg gcgctaaaga
     1501 aatcccggaa gatatgatga tagaggcctt ggatctggcg caggcggaaa taagaaagat
     1561 aacgactgaa ctggtgagga ttcgcgaacg cctcggcaaa ccaaaagccg aaattccttc
     1621 gcttccgtcg gtacccgaaa tcgacgagtg gatagccgaa gaatgtcttg aagacatacg
     1681 taaggccgtt cggatcaacg agaagaagga gcgcaacacg gccataggcg acgtcatcaa
     1741 acgagcctta gagcgcttcc tggaatccta tcccgattct gcggcatatg tggcctcgac
     1801 agtggaaggc cacgtgaaaa gggaactgcg ggccatgatc gttgaggaaa agaggcgtcc
     1861 agatggacga gccatgagtg aacttcgtcc gataagctgc gaggttgggg ttttgccgcg
     1921 cactcacggt tctgcgctct tcacgcgcgg ccaaacgcaa gctctcgtgg ttacgaccct
     1981 cggcatggta ggcgaggacg agcagatact cgacagcttg aagctggacg aaccgcccaa
     2041 gcatttcatg cttcactaca acttccctcc cttctcggtc ggagaggtcc gccccatgag
     2101 aggccctggg cgcagagaga taggccatgg cgcgcttgcg gagagagcca tagcgcctct
     2161 cgtccccgca gagggcgact tcccatatat agtgcgcgtg gtatcggata tcctggaatc
     2221 caacggttca agctccatgg ccagcgtctg tggagccagc atgagtctga tggatgccgg
     2281 agtcccgctc aaaaaggcat gtgccggtgt cgctatgggc ctgataaaag agggggacaa
     2341 ggtagagatc ctcacggaca ttcaagggtt ggaggatcac tacggtgaca tggatttcaa
     2401 ggtagcgggg acccgcgacg gcataactgc cctgcagatg gacaacaaag ctggcggcat
     2461 agatagaggg atactcgagc gagctctctc tcaggccaaa gaggcgcgac tggcgatact
     2521 cgatatcatg gagagcgcca ttccgtctcc g
//
LOCUS       DAOSAI010000352         2548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_26541_length_2548_cov_3.545527, whole genome shotgun sequence.
ACCESSION   DAOSAI010000352 DAOSAI010000000
VERSION     DAOSAI010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26541_length_2548_cov_3.545527"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..1364
                     /locus_tag="PLM83_10000"
     CDS             342..1364
                     /locus_tag="PLM83_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOK71773.1"
                     /translation="MSSLKRVLVVFLAAGMLVGCAFNGFAAPAPKVGIVFDIGGLGDQ
                     SFNDSANVGLERLKKELGYGTRYVESNTPSDYEPNLASLAREGYDVVWGIGFLMADAV
                     QKVAKQFPNTKFGIIDHGYSDAEYKEIPNVLGILYKEHEGSFLVGVLAALTTKTNTVG
                     FVGGMDIPVIHRFEAGYKAGVWKTNPNINIIVNYSGAFDDPGKGKTIAESMFRRNADV
                     VFHAAGSTGIGVIRAAEDHGKWAIGVDSDQNHLAPNNVLNSMIKRVDVGVFEGTKMLL
                     KPGFTGRTVVLGLRENAVGLAPEKSNRASAAATAKAREWANIIARGEYVVPEFPAEAD
                     KLYKKK"
     gene            1551..>2548
                     /locus_tag="PLM83_10005"
     CDS             1551..>2548
                     /locus_tag="PLM83_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71774.1"
                     /translation="MGRPQSVPVLEMRGITKQFPGVVANDNVDFKLEKGEIHALLGEN
                     GAGKTTLMKILYGLYEADEGMVLVNGKEISIKEPNDAISLGIGMVHQHFMLIPPFTVA
                     ENLVLGAEPVKNGLFMDMEKAIAKANEVSEAYGLAVDPNAKVVDISVGMQQRVEILKA
                     LLRGAEILVLDEPTAVLTPQEVTELFEIMRNLTEQGKSIIFISHKLKEVLDISDHVTV
                     LRRGKSIGTVATAETTQQELARMMVGRDVELVVEKGPAKPGKNILQVSELHVLDQRHL
                     PAVRGVSFSVRAGEIFGIAGVDGNGQSELVEAITGMRRPTSGRVVINGHDLTGARPR"
BASE COUNT          643 a          581 c          743 g          581 t
ORIGIN      
        1 gtacgatgta agtgtgcata catcgcaagt cgagttgctg acgagaaaga gcttgtgtta
       61 gttcggatgg cgcatcctta tggacgtggc tagttgagta actcgggaaa gcaaacgact
      121 gtacggataa ggtccgtggt atggagtttg acgcgccaag acgcggagca agattgtttg
      181 gagttgggaa gagaggacac tatgaggcta tttcgcatca accgctgacc gcgttgacat
      241 ggttgagcga tgaaactcag cccattactg ggaaggagtt ggccattaca tagcgaagtt
      301 taaccttaga ttcaaaccac cttaaagggg aggattattg aatgagctct ctcaaacgtg
      361 tcctagttgt tttcctggct gcgggaatgc tcgtgggttg tgcattcaac ggctttgcag
      421 ctcctgcgcc caaagttgga attgtatttg acatcggcgg tctaggtgac cagtccttta
      481 atgactcagc caatgtaggc ctcgagcgtc tcaagaaaga gttgggttat ggcactcgat
      541 atgtcgaatc gaacacacca tctgactatg agcctaacct tgctagcctt gccagagaag
      601 gttatgatgt cgtttggggt atcggctttt tgatggctga cgccgttcag aaggtagcca
      661 agcagttccc gaataccaag tttggaatca ttgaccatgg ctacagtgat gcagaataca
      721 aggagatccc caacgttctc ggaatattgt acaaagagca cgagggctcc ttcctagtcg
      781 gagtgctggc ggcgctgacc acaaagacga acactgtggg ctttgttggc ggcatggata
      841 ttccggtaat ccacagattt gaagctggat acaaggccgg agtatggaag accaatccaa
      901 acataaacat catagtgaac tacagtggcg cctttgacga tcctggcaag ggcaagacca
      961 ttgccgagtc gatgttccgc agaaatgccg acgtggtgtt ccacgctgcc ggttccaccg
     1021 gaatcggggt tatccgcgca gccgaggatc atggcaagtg ggccataggt gtcgattccg
     1081 accagaacca tctggccccg aataacgtct tgaactcaat gataaagaga gtggacgttg
     1141 gagtttttga gggcaccaag atgttgctga agccgggctt tacgggacgg acggtcgttc
     1201 tgggcttgcg cgaaaacgca gttgggcttg cgcccgaaaa gtctaacagg gcatccgcgg
     1261 cagccacggc caaggctcgc gaatgggcaa acatcattgc taggggcgag tacgtagttc
     1321 ccgagttccc tgcggaggca gataagcttt acaagaagaa gtagaagaga acgaggcagc
     1381 gatcttcggt agcggttttc ttatctgatt cgaacaatta gagaaccgtg aatcaaggta
     1441 ttgctgaaac agcgcgaacc taatgtagtc tgaggttggg cgccagctgc ttcagggtct
     1501 ggcgcccaac tgtgctatct tcataatgct atcttactgg agggactttt atgggaaggc
     1561 cccagagtgt cccagtgctc gaaatgcgcg gtattaccaa gcagtttcct ggagtcgttg
     1621 caaacgacaa tgtcgatttc aaattggaga agggcgagat ccacgcgctg cttggagaga
     1681 atggcgccgg aaagacgacg ctcatgaaga tcctctacgg attatacgag gccgatgagg
     1741 ggatggttct tgttaatggg aaagagattt ccatcaagga gcctaatgat gccatcagcc
     1801 ttggcattgg catggtgcat caacacttca tgctcatacc tcctttcacc gttgcagaaa
     1861 accttgtgtt aggagctgag cccgtaaaga atgggctttt catggacatg gagaaggcca
     1921 tagcaaaggc caacgaggta tccgaagcct atggcttggc tgtggacccc aacgctaagg
     1981 tcgtcgatat ttcagtgggc atgcagcaaa gggtggaaat actcaaggca ctgctgcgcg
     2041 gcgctgaaat actggtgctt gatgaaccta cagcggtgtt gactccccaa gaggtaactg
     2101 agcttttcga gatcatgcgg aaccttacgg aacaggggaa atcgatcatt ttcatcagcc
     2161 acaagctcaa agaggtgctg gatatatcag atcatgtgac tgtgctcaga cggggcaagt
     2221 ccataggcac agttgcgacc gccgaaacga ctcaacaaga acttgccagg atgatggtgg
     2281 gccgcgatgt tgaactcgtc gttgaaaagg ggcccgcaaa gccgggcaaa aacatactgc
     2341 aagtgagcga gttgcacgtg ctcgatcagc ggcatcttcc agcggttcgt ggagtctcgt
     2401 tttcagttcg tgcaggagaa atcttcggga ttgccggcgt agatggaaac ggccagagcg
     2461 agctcgttga ggcgatcacc gggatgcgtc ggccgacatc cgggcgcgtt gtcattaacg
     2521 gtcatgatct taccggcgct cgcccacg
//
LOCUS       DAOSAI010000353         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_26568_length_2546_cov_2.854275, whole genome shotgun sequence.
ACCESSION   DAOSAI010000353 DAOSAI010000000
VERSION     DAOSAI010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26568_length_2546_cov_2.854275"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..858
                     /locus_tag="PLM83_10010"
     CDS             130..858
                     /locus_tag="PLM83_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71775.1"
                     /translation="MAFEQWTDRPAHDCDGEYLRLRKDILSDFDAVRRQLRIIPEPGS
                     TWGYGFDLRFAMLLYENLSKKYSFSQRLASDDGVWRYLSIRVLPDIVYLRWGLTPSRF
                     YSEPRRVWLRALWWYIHLSWQGTVEETLRILEGNTTDDIVQVVERPGPHGYRIELTRT
                     IMREYHRVKSMHSVEPRLLRKIMVLNTARLRVVEPSLCSGGEDAYVKELFRYFGYDEQ
                     RVAAGNRSVLGLRWPFVRISGGRV"
     gene            776..2155
                     /locus_tag="PLM83_10015"
     CDS             776..2155
                     /locus_tag="PLM83_10015"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOK71776.1"
                     /translation="MSNGLPQVIDLFSGCGGLSLGFQAAGFSILGGIDVDETAVDTAA
                     YNLSWRFGFEGFHLCCDIRHVDGEVFSNIERTGPIVVVGGPPCQAYSLVGRAKLRSLG
                     REREHTNDHRGYLFEDFIRFAEQLDADVVVMENVPESIDYGGLNVPEHIAECLNTIGY
                     RAKWTILNAADYGVPQVRERVFVVAFRDNYDGNFCFPVPTHAPTFDLPSYVDRQVSRF
                     HGLKYFVPPPEYPKDVVPWVTVEEALSDLPVLFSTAQSRYRFLKPSIRLPYKTSAKCE
                     YQRLMRSWYGTKTTGVTGHCFRRNERDFRIFERMQEGDDYSDASVIADKLLAEAARAQ
                     GVSPLNSPAEFEELRKKIVPPYSRTKFLNKWKRLTRNQPSHTLTAHLGIDTYSHIHPW
                     EPRGISVREAARLQSFPDEFLFLGSMNDAFRHIGNAVPPLLAKAIADQILVEIIRMER
                     GYALHEQTS"
     gene            2139..>2546
                     /locus_tag="PLM83_10020"
     CDS             2139..>2546
                     /locus_tag="PLM83_10020"
                     /inference="COORDINATES: protein motif:HMM:NF025748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK motif protein"
                     /protein_id="HOK71777.1"
                     /translation="MSRLVDEIRSLFATTRTHKASVVESLSPTDIAWVVRFSDCYGVA
                     VPYLDNETVSERFHSARLSTRVIIIDGAEHRCLLLTSRDETYRNEFAVLCADFIEPGT
                     SGEKRRELLQNPLRWWERWTGLLGNVASSKTAHG"
BASE COUNT          628 a          543 c          693 g          682 t
ORIGIN      
        1 tttattacca ccctcgattg gaagacggac tcgccagaac ggctggcctt gtcgatcagg
       61 gagtatatag acaaggggat gtgatagagc gtgttgaact ggaagaagct aacgaagaag
      121 gaggccgcct tggcttttga acaatggact gacagacctg cgcatgattg tgatggggaa
      181 tacctgcgtc ttaggaagga tatcttgagc gattttgatg ccgtccgcag acaactcaga
      241 ataataccgg aacccggcag cacttggggt tatggttttg acctccgatt tgcgatgctg
      301 ctctatgaaa acctatcgaa gaagtatagt ttttcgcaac gcttggcttc tgacgatggt
      361 gtatggagat atctttcgat tagggtgctt ccggacatag tctatcttcg gtggggctta
      421 actcccagcc gtttctatag cgaacctcgg agagtgtggc tgagagcgct ttggtggtac
      481 attcacttgt catggcaggg gacggttgaa gagacgctcc gtattctcga aggcaatacg
      541 accgatgata tagttcaggt ggttgagcgg cctggccccc acggctatcg catcgagctt
      601 acaagaacta tcatgagaga atatcatcgt gtgaagtcga tgcacagtgt tgagccgaga
      661 ttgcttcgca aaatcatggt tctaaacacc gctagactaa gagtagtgga accatcactt
      721 tgttctggtg gagaagatgc atacgtaaag gagttgttca gatactttgg gtatgatgag
      781 caacgggttg ccgcaggtaa tagatctgtt ctcgggttgc ggtggccttt cgttaggatt
      841 tcaggcggca gggtttagta tattgggtgg cattgatgtt gatgaaacag ctgttgatac
      901 agctgcgtac aatttatctt ggcggtttgg cttcgaaggg tttcatctct gttgtgacat
      961 taggcatgtc gacggcgaag ttttctcaaa catagagaga acgggaccca tcgtggtggt
     1021 aggcgggccg ccgtgtcagg catattcgtt ggttggtcga gccaaactcc gttcgctggg
     1081 ccgtgaacgc gaacatacta acgatcatag gggttacctt ttcgaggatt tcataagatt
     1141 tgccgagcaa ttggacgctg atgtcgtggt tatggagaat gttcctgaat cgattgatta
     1201 tggaggcctg aatgtcccgg agcatatagc agaatgcctt aataccattg ggtatcgagc
     1261 aaagtggaca atcttaaatg cagccgacta cggtgtaccc caggtgcgcg agcgtgtctt
     1321 tgtggtagcc tttagagata actatgatgg aaacttctgc tttccagtgc ctacgcatgc
     1381 accaacgttt gatcttcctt catatgttga caggcaggtt tctcgttttc atggtctgaa
     1441 atactttgtt cctccccctg aatatccgaa ggatgtagtg ccttgggtta cagttgagga
     1501 agctctcagt gacctaccgg ttctcttctc tacagcgcag agtcgctatc gatttctaaa
     1561 accctctatt cgtttgccgt acaagacatc tgcaaagtgt gaataccaga ggctgatgcg
     1621 ctcgtggtat gggacaaaaa cgactggcgt cacagggcat tgttttcgcc gtaatgagag
     1681 ggactttcga atttttgaac gtatgcagga aggtgacgac tacagcgatg catccgtgat
     1741 tgcagataag ttgctggctg aagctgcccg ggcacaggga gttagtcctc ttaattctcc
     1801 ggcagagttt gaggagcttc ggaagaaaat cgttccacca tacagcagaa ccaagtttct
     1861 aaataagtgg aagagactta caaggaacca gccttcacac acattgacgg cacatttggg
     1921 tatagatact tacagccata ttcacccgtg ggagccacgt ggtataagcg ttagagaagc
     1981 agctagactg caatcgtttc ctgacgagtt cttgtttctt gggagcatga atgatgcttt
     2041 tcggcatatt gggaatgcgg ttccacctct cttagccaag gccattgcag atcaaattct
     2101 agtcgaaatt atacggatgg aaaggggata cgccctccat gagcagacta gttgatgaaa
     2161 ttcgctcttt attcgctaca acaagaactc ataaagctag tgtcgtagaa agtctgagcc
     2221 ccactgatat tgcctgggtc gtacgctttt ctgattgcta tggtgtagct gtaccgtatc
     2281 tggacaacga aacagtgtcg gagcgattcc atagtgcgcg tttatccacc agagtaatta
     2341 ttatcgacgg ggcggaacac cgatgcttgt tgctcacatc acgtgatgag acataccgaa
     2401 atgagtttgc tgttctgtgt gcagacttca tcgagccagg taccagcggt gaaaaacgcc
     2461 gggaactgct ccaaaaccca ctgcgctggt gggaaaggtg gacaggcctg ctgggcaatg
     2521 ttgcatcttc caagactgct catggt
//
LOCUS       DAOSAI010000354         2542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_26613_length_2542_cov_3.469240, whole genome shotgun sequence.
ACCESSION   DAOSAI010000354 DAOSAI010000000
VERSION     DAOSAI010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26613_length_2542_cov_3.469240"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..892
                     /locus_tag="PLM83_10025"
     CDS             173..892
                     /locus_tag="PLM83_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71778.1"
                     /translation="MTDGNSTFLTLLNSRRIVLIMSLPGNEPELASAAIDSGADVLKV
                     HINLEHRASGLKFGTLEEERSNLEKILDLAGKSGVPVGIVPGAMSVIDAGQIQEVEAF
                     GFDFVSAYAHHLSPAVLSGAVTSRVAKMIAADSTYSVEEIEAMARVEFDIVEASIIPP
                     EGYGEPLSCRDLLSYQNIVGWTGKPVVVPTQRRISPEDVPALVATGIRAIMLGAVVTG
                     CTPENIATQVAVFRRSIDELF"
     gene            920..2278
                     /locus_tag="PLM83_10030"
     CDS             920..2278
                     /locus_tag="PLM83_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK71779.1"
                     /translation="MKEQTYETDVAVIGGGCSGIAAAIAAARIGANVIIVDRLGFLGG
                     MATAALVSPMMTFHAKSGQIIDGIGQQLVDRLHEQGFSPGHVPDTIGFVPTVTPIDPE
                     GFKLIAMEMVLEAGAKPLLHQLVVDVDSDNGKVKSVIAVGLTSLSRIKASVFIDCTGN
                     ARLVELGGGELACGREADSLTQPATMIFRVGNVDLHEIVDFIKANPEDFILGCAPQDL
                     DAEQPLAVAGFFGKVREGVEHGVFHVDRDRLLLFSGVMPGEVVVNVTRVKVLDPADPV
                     DITRAEIEGRRQVYDVFQFLKTRIPGFSAAQLIGTGCQIGIRETSRPVGDYILTQEDL
                     LSGRDFADSVARGAFPIDIHSPDGKGLTALKLDRSYAIPYRCLRPKGLTNLLCAGRAI
                     SVTHEAYASTRVMPTCLATGQAAGTAAAIAIGREGDVRDIDIDVLRATLVRNGAILYE
                     PS"
BASE COUNT          628 a          534 c          727 g          653 t
ORIGIN      
        1 tgctgagcct ggaactagaa gatatggtca agtccgcaag gagcgttttt gaccactatt
       61 tcgctagttg tcctgcgggg gagtttcgac tgactgagat ctgctgcgag aacccgtggg
      121 ataggacttt tgagtgtaga gtaaacgcca caattgagga ggtagatgcc ttgtgacaga
      181 cggaaacagc acgtttctga cattgcttaa tagccgtcgc attgttctaa ttatgagtct
      241 tccaggcaat gagcctgagc tggctagcgc tgcgattgac tcaggagctg atgttctaaa
      301 agtgcacatc aacctggaac acagggcttc tggtctgaag tttggcactc ttgaagagga
      361 gcgttccaat ctcgagaaga tcctcgacct tgccggaaaa tcaggcgtgc ccgttgggat
      421 agtaccaggt gctatgtcgg tcatagatgc ggggcagata caagaagtag aggcctttgg
      481 atttgacttc gtttctgcat atgcacatca cctttccccg gcggtgctgt caggagctgt
      541 gacttcacgt gtggccaaga tgattgcagc agattcgaca tactctgttg aagagataga
      601 agccatggca agggtcgagt ttgacatagt ggaagcatcc attattcctc cggaaggata
      661 tggggaacct ctgtcttgcc gagatctact gtcgtatcag aacattgtgg gctggacggg
      721 taagcccgtg gtagttccta cccaacggag aatctctcca gaggatgtgc ctgctctggt
      781 ggctactgga attcgtgcaa taatgcttgg agctgttgtt actgggtgca caccggaaaa
      841 tatagctaca caagtggcag ttttcaggcg aagcatagat gaactgttct aggctggtta
      901 ggcaattggg ggcatgctgg tgaaagaaca gacttatgag accgatgtgg ctgtaattgg
      961 aggcggctgc tcggggatag ctgcggcaat tgcagcagct cgtatcggag ctaatgtcat
     1021 aatcgtggat aggcttgggt ttcttggagg catggccaca gctgccttgg tgagtcccat
     1081 gatgactttt catgccaagt ctggacagat cattgatgga attggccagc agcttgtaga
     1141 taggcttcac gaacaagggt tttctccagg tcatgtgcca gacacaatag ggtttgtgcc
     1201 aacagtcact cctattgatc cggagggttt caagctgatt gccatggaga tggtgcttga
     1261 agctggcgca aaacctcttc ttcaccaact ggtagtggac gtggattccg acaatggcaa
     1321 agtgaaatcg gtaattgcgg tgggtctgac atccctaagc cgaataaagg catctgtctt
     1381 catagactgc acggggaatg cacgtcttgt ggagctgggt ggtggagaat tggcgtgtgg
     1441 acgtgaggct gattcactaa ctcagccggc aaccatgata tttagagtgg gaaatgttga
     1501 tctacatgag attgtagatt tcattaaagc caatcccgag gatttcatat tgggatgcgc
     1561 gccccaggac cttgatgccg agcaacccct agctgtagca ggtttctttg gcaaagtgcg
     1621 tgagggcgtc gaacatggag tgtttcatgt tgaccgtgac aggcttctgc ttttttcggg
     1681 cgtcatgccg ggagaagtcg ttgtcaacgt gacccgagtc aaggttttgg atcctgcaga
     1741 tccggtcgac attacgcgag cagagattga aggtagacgg caggtttatg atgttttcca
     1801 gttccttaaa acccgcatac ctggtttttc agcagcacag ctgatcggta caggctgcca
     1861 aatcggaatt agagagacgt ccagaccagt gggagactac atactaaccc aggaagacct
     1921 cttgtctgga agggattttg ctgactcggt agcccggggc gccttcccca tagatattca
     1981 ttcacctgat ggcaagggcc tcactgcatt gaaacttgat aggtcatatg ctatccctta
     2041 tcggtgtctc aggcctaagg gattgacgaa tcttctatgc gcaggaagag cgatatctgt
     2101 aacacatgag gcttatgcat ctactcgagt gatgcctaca tgtttggcta cgggtcaagc
     2161 agccggaaca gctgccgcta ttgcgatcgg aagagaagga gatgttcgcg atatcgatat
     2221 agatgttttg cgtgcgacac ttgtgcgcaa tggagccata ttgtacgaac catcttaaga
     2281 aggcacattc gcttgagaag gggtgatatg aaggccggaa catacgtctg gtgaccatta
     2341 aggcaggtat tacttacgta gactatcgca agattgagaa agaagggatt agtgatgaag
     2401 cgggttttca cagtgattgc ggcggttgta gtaatgttga gcctgaccag tatagggtat
     2461 gcaaaaggta ccaccataaa cttatggttt gctggtactg aagcaccctt catgaaaact
     2521 gtaaacgaag agatcttgcc at
//
LOCUS       DAOSAI010000355         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_26836_length_2527_cov_3.226942, whole genome shotgun sequence.
ACCESSION   DAOSAI010000355 DAOSAI010000000
VERSION     DAOSAI010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26836_length_2527_cov_3.226942"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="PLM83_10035"
     CDS             <1..1180
                     /locus_tag="PLM83_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK71780.1"
                     /translation="AQELTGPEYAEHFINKAFDSGVETMLDTMVLDVTRDRQIIAINS
                     DEGLMRIDAEAIILAMGARERNRGAVTIPGTRPAGVFTAGAAQRFINMEGYMPGRRVV
                     ILGSGDIGLIMARRMTWEGAKVEAVLEILPFPSGLTRNIVQCLDDQGIPLYLSHTVIS
                     VKGEERVEGVTVARVDENLKPVPGSEWDIECDTLLLSIGLIPENELSREAGVELDAVT
                     GGPVVTDEMETSVPGIFACGNVVHIHDLVDNVTREAWAAGEGAARLVTGKQPRGATIR
                     TRAGDGIRYVVPHVVHVQNLADRDVRLFMRATHPAENVRVEVVVGDNVLFSKFERVVR
                     PAEMLALTIPAGRISEEAIASASLADAGRRGEEGSKNGPELVVRVTPRDVHKRGGVQA
                     "
     gene            1189..1641
                     /locus_tag="PLM83_10040"
     CDS             1189..1641
                     /locus_tag="PLM83_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOK71781.1"
                     /translation="MDTAVITDKTKEIRELTCIVCPVGCSLQATIEDGNVLDVEGNSC
                     KRGREYAVDELCAPKRTVTSTVRVEGGFLPLVPVRTAAPIPKEKIDQALLEVAKATLE
                     APVRIHQVIIADVAGTGVDVIASRSMTKTVGTYPAFGAKTVLVHDACV"
BASE COUNT          546 a          606 c          795 g          580 t
ORIGIN      
        1 ggcgcaggag cttacagggc cggagtatgc ggagcatttt atcaacaagg catttgattc
       61 cggagtagag acgatgctcg ataccatggt cttggatgta acacgcgaca gacagataat
      121 cgccatcaac agcgacgaag ggctgatgag gatagatgcg gaggcaatca tactggctat
      181 gggcgctagg gagcgcaacc gtggggcagt aactatcccc ggaacgcggc ccgcgggagt
      241 tttcactgcc ggggcggcgc agcgatttat caacatggag ggatacatgc ctggcaggcg
      301 cgtcgtgatt ctagggtcag gagatatagg cctgatcatg gcgaggcgta tgacgtggga
      361 aggcgccaaa gtcgaggcgg ttctcgagat actgcctttt ccttcaggct tgacgagaaa
      421 catagtccaa tgcctggatg accaggggat tccgctttac ctgagccaca ctgttatctc
      481 cgtgaaagga gaggagaggg tcgaaggtgt gaccgtggcc agggtggacg aaaacctcaa
      541 gccggtgccc ggctccgaat gggacatcga atgcgacacg cttctgcttt cgatcgggct
      601 gatacccgaa aacgaactga gccgtgaggc cggcgtagag ctggatgcgg tcactggagg
      661 acctgttgta acagatgaga tggagacatc agttccgggt atctttgcat gcggcaatgt
      721 agtgcacatc catgacctgg tcgataatgt taccagggag gcctgggcag ccggtgaggg
      781 tgcggcaagg ctcgtgaccg ggaagcagcc gcgaggtgcc acaattcgca ctcgcgccgg
      841 agacgggata aggtatgtgg ttccacacgt agtccatgtg caaaacctcg ctgatcgcga
      901 cgtgcgtctc ttcatgcggg caacgcatcc tgcggagaac gtgagggtgg aggttgtggt
      961 cggcgacaat gtgttgttca gcaagttcga gcgtgtcgtg cgtcctgcgg agatgctggc
     1021 ccttactata ccagcgggaa gaatctctga ggaggcaata gccagtgcga gcttggccga
     1081 tgcgggccgg cggggagaag aaggctcgaa aaacggtcct gaacttgtgg tgcgcgtaac
     1141 accgcgtgac gttcataaga gaggaggagt acaggcatga gtgcagagat ggacacggcc
     1201 gtcattactg acaagacaaa ggaaatcagg gagcttacat gcattgtatg tcctgtcggt
     1261 tgcagtctgc aagccactat tgaggatggc aatgtcctag acgttgaggg caattcctgt
     1321 aagcgcgggc gtgagtacgc agtggatgag ctgtgcgccc cgaaaaggac tgttacttcc
     1381 acggtgaggg tggaaggcgg gtttcttccg ctcgtgcctg tgcgcactgc tgcgccgatt
     1441 cccaaggaaa agatcgatca ggctctgtta gaggtcgcaa aggctacctt ggaggcgcct
     1501 gtaagaatcc atcaggttat cattgcagac gtggcaggca ccggcgttga cgtgattgcc
     1561 agcagatcca tgacgaaaac tgttggcaca tacccggctt ttggtgccaa gacggttctg
     1621 gttcatgatg catgcgtgta acttggcatt ggcgcggtcc tgtgccgcgc ctgatttcat
     1681 gcctgaatat atgccttttt catgaggcta tttgcgtagt atgcgggata tagtaggcaa
     1741 cggctgctct tcagtctacc aaaacgagcc atgtggtcgc gtaaggccct cgcagttttt
     1801 gtcgttcctg tcattgctgt aggggttatg cctgtcgtgg cgtcttggga tgcactggct
     1861 agcgggggcg agatatattc gtagcggagc ttgaagaggc cggctgtcct acacgtgagg
     1921 ctgccaattt cttaaagctg atcgcgattg ccttgttccg gcagatgagc ggcgcctacg
     1981 atgcggaact gattgctcat acgtggggca cagcttccca cgagacctag tattgatatg
     2041 tatcccctcc atttcctatc aggctgcccc aaccaggatg cgctggttct tcccatttcc
     2101 atagctttcc tcggtgacaa gactagcagc atctttgtgt ccacgcattc cctgacgatc
     2161 tcctgaccga cggaaatcgg gtttcttttg tgggtacacg ggcctgctgg gttcccgaac
     2221 tacctgtgag gatagaaacc gctgtcactc tcctttatct gtgcacggca gatgggctgg
     2281 gggtgttgtt gagtgttgaa ggagaaatgc gtttttcagg gatatggctg acaaatgctc
     2341 ttctcggtcg agttaacccc tgatcatttc cgttctcttg agcttgccgg cagaagaaat
     2401 gttgccaggt ctttatgcgg gagacagatt cccgagctcc aaggctcaga cgggatgagg
     2461 ctcttgccac tgtgagtagg tggtctgctg aagccgaacg gccttatgca ctggcgaagt
     2521 cactgct
//
LOCUS       DAOSAI010000356         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_27063_length_2512_cov_2.711437, whole genome shotgun sequence.
ACCESSION   DAOSAI010000356 DAOSAI010000000
VERSION     DAOSAI010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27063_length_2512_cov_2.711437"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1621
                     /locus_tag="PLM83_10045"
     CDS             248..1621
                     /locus_tag="PLM83_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017714397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOK71782.1"
                     /translation="MQNLIKDALKSHRADYVEIRIEDHERTKISLRGKNVDELATSRS
                     VGGCVRALVSGGWGFVSFNDIANLKDHVALAVSQAKLVSRVCGDNLLAPVEPITDTVV
                     PALVVDPRSISLASKRALLEEYNDIMLSYSPKIQTTAVRYRDTYSKKYFANSECTYIE
                     QEHCDVGASFTAIAREGDNVQQMQVSAGGIDGYQCIKGHHDEVRECARRAVENLSAAP
                     VDAGQYTVVLDPGLAGVFVHEAFGHLSESDFIYENSMLREIMVLGKQFGSPILNIADG
                     AAVPGHRGSYKYDDEGVRSSVTPLIRDGVLVGRLHSRGTAAKMSERPTGNARAINYRF
                     QPIVRMTNTYIENGETSFDEMISDIDKGIYAIDAYGGETTMEMFTFSAMEAFMIRKGK
                     VAERVRNVVLTGNVFETLMHIDAIGNDRKMNDGGVGGCGKGQQFPLPVSDGSPHIRIK
                     NVVVGGN"
     gene            1624..>2512
                     /locus_tag="PLM83_10050"
     CDS             1624..>2512
                     /locus_tag="PLM83_10050"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOK71783.1"
                     /translation="MKDLLDVAIKNADAAELYLVNSETIPVVFEMSKLKQAETKRTTG
                     VALRIIRSGKIGFSSSTDLSKAAADNLVNMATESADMGETARFDFPGPITDGEVMPAP
                     LIYDDRIADIDADELIRMGQEIVDIITEEDPSIKCGVQLEREVQEVTITNSNGGGGRY
                     RKTTLTAMADALLVEGTDIFLIYSMAGSCCSNFDSRSIAQDVLERVRLGRTIAQARTG
                     RMPVLFTARGIMPLFIALQSALNGKTVLEGSSPLADKVGRQVFDPRVFVYDDATVDCA
                     LGSCPMDAEGVKSRKTPLIE"
BASE COUNT          683 a          680 c          660 g          489 t
ORIGIN      
        1 gcctagcaga catcatatgg caggctaacc gatagcgcaa aaacttattg ggagtcactt
       61 agccacatct gttcgattgc caaccgcaag ttcggacagg gcatatgggt cgccacacta
      121 ctgaaggata atcaagctca atgtttaagt tctgcacatg ctcccaaagc aaagcgcgac
      181 attcccatcc aaacatatct cccgcaaacc aggcaaaaag ctcagcccaa aaaggaggca
      241 caagatcttg cagaacctga tcaaggatgc gttaaaatcg catcgagcgg actatgtgga
      301 aattagaatc gaagaccatg aacggaccaa gatatcgctt cgtggcaaga acgtagatga
      361 attggccacg tcccgctcag taggaggctg cgttcgcgcc ttggtatccg gcggttgggg
      421 attcgtctcc ttcaacgaca tcgccaacct caaagaccac gtggcgctgg cagtaagtca
      481 agcaaaactg gtatcgcgcg tgtgcggcga caatctcctt gccccagtag aacccataac
      541 cgacacggta gtgccggcat tagtcgttga ccccagatca atcagccttg caagcaagcg
      601 agcactgctt gaggaataca acgacattat gttgagctac agtcccaaga ttcaaacaac
      661 tgcagtcaga tacagagaca cttactcaaa gaagtacttc gccaactccg agtgtacata
      721 catcgagcaa gagcattgcg acgtcggagc cagcttcacc gccatcgcac gggagggcga
      781 caatgtgcaa cagatgcagg tctccgccgg aggaatcgac ggttaccaat gtattaaagg
      841 gcaccacgac gaagtcagag agtgcgccag acgtgcagtt gaaaacctca gcgcagcacc
      901 tgtggatgcg ggccagtata ctgtggtact cgaccccggc ctagcaggcg ttttcgtgca
      961 cgaggccttc ggacatctaa gcgaatctga cttcatctat gagaatagta tgctccgcga
     1021 aatcatggtt ctggggaagc agtttggctc cccgatcctc aacattgcag acggcgccgc
     1081 cgttcccggc catagaggat catacaaata cgacgacgag ggcgttcgtt cctcggttac
     1141 tcccctcata agagacggag tgctcgtcgg cagactgcat tcccggggaa cggccgcgaa
     1201 gatgagcgaa cgccccactg gaaacgctcg cgccataaac tacagattcc aacctatagt
     1261 gcggatgacc aacacttaca tcgaaaacgg agagacaagc ttcgacgaaa tgatctccga
     1321 tatcgacaag gggatctacg ccatcgatgc ctatggcgga gagacaacta tggagatgtt
     1381 tacattcagc gcaatggaag ccttcatgat ccgcaaaggg aaagtggcgg aaagagtccg
     1441 caacgtggtt ctcacaggca atgtctttga aacattgatg cacatagacg ccatcggcaa
     1501 cgaccgcaag atgaatgatg gcggtgtcgg agggtgcggc aagggccaac aattcccatt
     1561 gcctgtctca gatggcagcc cgcatatacg gatcaaaaat gtcgttgtgg gaggaaatta
     1621 gccatgaaag acttgctcga tgtggcgatc aagaatgcag acgcagctga gctctacctt
     1681 gtcaatagtg agaccatccc cgtggtcttc gagatgagca agctcaagca ggccgaaacg
     1741 aagaggacta ctggagtggc tctgcggatc atccgttctg gcaagatagg cttctcctcc
     1801 tcaacagacc ttagcaaagc cgctgccgac aatctcgtca acatggcaac ggagtccgca
     1861 gatatgggtg aaacagcaag gttcgacttt ccaggcccga tcaccgacgg agaagtaatg
     1921 cctgcgccac tcatttacga tgacagaatc gcagacatcg acgccgatga actcatccgt
     1981 atgggccaag aaatcgtcga tatcatcaca gaagaagacc cctccatcaa gtgcggtgtt
     2041 cagcttgaaa gagaggttca agaagtcacg atcaccaact cgaacggagg aggagggcgc
     2101 taccgcaaga ctactctgac tgccatggcc gatgccctgt tggtagaagg aaccgacatc
     2161 ttcttgatat actccatggc cggttcatgc tgcagcaact tcgactcccg ctccatagct
     2221 caggacgtgc tagagcgcgt gaggttgggg cgcactatcg cccaagcacg aacaggccgc
     2281 atgccggtac tcttcacggc ccgcggcatc atgccgcttt tcatcgcctt gcagtcagcc
     2341 cttaacggaa agacggttct tgaaggttca tcaccgttgg cagacaaagt aggccgccag
     2401 gtgttcgacc caagggtttt tgtatatgat gacgcaactg tcgactgcgc cctcggaagc
     2461 tgcccaatgg atgccgaggg cgtaaagtcg cgcaaaacac ccctgatcga gg
//
LOCUS       DAOSAI010000357         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_27077_length_2511_cov_3.879479, whole genome shotgun sequence.
ACCESSION   DAOSAI010000357 DAOSAI010000000
VERSION     DAOSAI010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27077_length_2511_cov_3.879479"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /gene="thiL"
                     /locus_tag="PLM83_10055"
     CDS             complement(<1..652)
                     /gene="thiL"
                     /locus_tag="PLM83_10055"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOK71784.1"
                     /translation="MEISKIGEFGLISRIDDGCVNDPSSVVQGIGDDAAVVRVSPGCM
                     LLICTDMLVEDVHFIRCAAPWTHIGYKAVAVNLSDIAAMGGEPKHCVVSIALPPSERV
                     ENVDRLYEGMKCLLAKDRVNLVGGDMVESSILAISVTMVGQADEKKIKYRYGARPGDV
                     VMVTGDLGASAAGLHLLEAELKSGTWTINSAPDMEEQPHQEDPRSTALRAHFLPVPR"
     gene            complement(1415..>2511)
                     /locus_tag="PLM83_10060"
     CDS             complement(1415..>2511)
                     /locus_tag="PLM83_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK71785.1"
                     /translation="RDPAPDEPTGLLMNSAQDLIAIPPYTDQEVERGFALALGQLPKW
                     GITTVHDMLVDADAFATYQRLAESHSLPARVRVWLEGITFGSRDAGLIPFVLGSGLKS
                     GYGNDMLKFMGIKFILDGSIGGCSAAVEEDYAGRKGEKGVLYVERDELSPLVTECVKN
                     GIRVSIHAIGERAIELAISSLEDAAREVSVESVRAMRNRIDHCVLPTRDHLKRIRDLG
                     LVIEASTSFMYALGDSWISNLGYERACRTFPLRSAIDIGIRVAGNSDSPVCDGSPLLG
                     MYAAVTRKTSSGMTLGNSEKMTVDEALRSYTAIGAYAGYEENLCGSISPGKSGDLVVL
                     SENPRSVPPEHLKDIDVEMTFVGGVKVFSK"
BASE COUNT          579 a          762 c          595 g          575 t
ORIGIN      
        1 gcctcggaac cggcaggaaa tgagcgcgca aagcagttga acgggggtct tcttggtgag
       61 gctgctcttc catgtccggc gccgaattga tagtccatgt tccacttttc agctcggcct
      121 cgagaagatg caggcccgct gctgacgcgc ccaggtcccc ggtgaccatc actacatcgc
      181 cgggccttgc gccatagcgg tactttatct tcttctcatc tgcctggccc accatggtca
      241 cgcttatcgc aaggatgctg gattcaacca tgtcgccccc gacgagattg actcggtcct
      301 tcgcaagcag gcacttcatt ccctcatata gccggtccac attctccacc ctctccgatg
      361 gcggaagagc gatcgacact acgcaatgct taggctcgcc gcccatggcg gcaatatcgc
      421 tcaggtttac agccacagcc ttgtatccta tatgtgtcca gggagcagcg catcttatga
      481 aatggacatc ctctacaagc atatcagtgc atataagaag catgcatccg ggggaaaccc
      541 tcacgacagc cgcatcgtct ccgattccct gaactactga ggacggatca ttcacgcatc
      601 catcatcgat ccgggatatc aggccgaact ctcctatctt cgaaatctcc atcgaaatct
      661 gctcctttgc agcgctccat agcaaccatc acacacaggc atccaagtgg gtagagagtg
      721 cactcggggg aatacggctc atcaatttcc tacagataga actatagcat ttccgtctcc
      781 tgatcatgac catatcggac ctgtgtataa accaagaagg cgaacatgat tcctgtagcc
      841 aggataatac cgcggcgctt cgaatgcttt ccccacccac cccctgctcg ggacttatcg
      901 ccagtgccct ggtcatacaa atcagtccag gaaatgtcgg catttaacat agatcctttg
      961 cagccggcta tagcacatat gcgatggttt gcagcaacag gagtgttctc aggcttcgcg
     1021 ctttttcatg cgcatgggcg ctgatcctgc gtcgtctggc ggcatgaaac tgggctccaa
     1081 ctaacaggcc tcactcaatc tgaacaaagc cggtaatggg ataccgggcc cgccaggaca
     1141 aagttggggc tttgaaggtt attttccaag ctgtagcagg caataacggt cttccagcct
     1201 ggacaggcac atgctcctat gccggaaaca ggcttcggtt tgatcacccg acaatcccct
     1261 tccttcaggc atggtgagtg tcacggcccg gaggcatgca atgtgacaag gacctggcgc
     1321 cgccccgggc aaggcctgtg cagcccccgc ggggtcagac gccatcctct gccggcatgc
     1381 tctctccact gatccggttc aggtaggagc aagattactt ggaaaagact ttgactcctc
     1441 cgacgaatgt catttcaaca tctatatcct tcaggtgctc gggaggaacc gacctgggat
     1501 tttccgacaa gacaactaaa tcgccagact tgccagggct gatgctgcca caaagattct
     1561 cctcataacc tgcataggca cctattgccg tatacgaacg gagagcttca tccactgtca
     1621 tcttctctga attaccgagt gtcataccgc tcgaagtctt ccttgtcacc gccgcataca
     1681 tccccagaag aggactgcca tcgcacaccg gcgaatcgga attgccggcc acccgaatgc
     1741 ctatgtctat agcgctcctt agagggaatg tgcggcatgc cctctcgtag ccgaggttcg
     1801 atatccagct gtcgcccaaa gcatacatga aagacgtgga cgcctcgatc accagcccaa
     1861 ggtctcggat cctcttcaag tggtctcgag taggcaatac gcaatgatct atcctgtttc
     1921 gcatagctcg cacactctct acggagacct cccgagcggc atcctccaaa gaggatatag
     1981 caagctctat ggccctttca cctattgcat ggatcgatac cctgatgcca tttttcacac
     2041 actctgtcac aaggggtgaa agctcatccc tttcaacata gagaacccct ttttcaccct
     2101 ttcggcctgc ataatcctct tccacagctg ccgaacaccc gcctatgctg ccatccagga
     2161 tgaattttat gcccatgaac ttcaacatgt cgtttccata cccgctcttg agtccggatc
     2221 ctaaaacaaa ggggataagc cccgcatccc gcgacccgaa agtgatgcct tcaagccaca
     2281 cgcggacccg agcaggcaag ctatggcttt cagctagtct ttggtaggtt gcgaacgcgt
     2341 ccgcatcgac gagcatgtcg tggactgttg ttatccccca cttcggcaac tgcccaagag
     2401 cgagtgcgaa tccgcgttcc acttcctgat cggtgtacgg cggtatggct atcagatctt
     2461 gtgcactatt catcaacaaa ccagtgggtt catcaggggc aggatctcgg t
//
LOCUS       DAOSAI010000358         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_27371_length_2492_cov_4.261387, whole genome shotgun sequence.
ACCESSION   DAOSAI010000358 DAOSAI010000000
VERSION     DAOSAI010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27371_length_2492_cov_4.261387"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..1306)
                     /locus_tag="PLM83_10065"
     CDS             complement(380..1306)
                     /locus_tag="PLM83_10065"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOK71786.1"
                     /translation="MSDEQTTRAEKIEFRCACGTRFRVDARLSGRRGRCNTCGSSLVI
                     PQVSTQDPVASQPKHIPGETKSEQAEEARDEVAVEALCSICQSPIEEDDEVIQCSECH
                     LPFHAECWQENLGCSTYGCSQVSILRQGPDVKIEGTSMASPVDASDNQPIWHCAIGTQ
                     FYGPVTATELAKWAQENRITTGTMVWKGGMDNWQPIASMPDLAAIVDPVYAARSLNAG
                     FPWKLVLLAASAIGFLLSLVSYGIPSLLLLLSVGLLGVLRLRKSNLLSGQSGASGTVY
                     SKPLHWKSIALLVLVAAVSLTGFVAGLSVGLD"
     gene            complement(1349..>2492)
                     /locus_tag="PLM83_10070"
     CDS             complement(1349..>2492)
                     /locus_tag="PLM83_10070"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOK71787.1"
                     /translation="TAAQQVTAIQATVPGKTSIWYNSLFYMAVFGLLGGLVAWGVGEA
                     VYQGMSSPLDEYMEFLGKANTIFVQARDGDISEVRAEAQLEALAAEYDDNPYIRIARD
                     DSMSDFQKEAARDRQLEKDKRHSQLKSIMFIGLVGLILAMSLSIAEPVVSRNWRSAGI
                     SSCVGIFLGLIGGVIVGLFVDHLYRALGAGSDSTGKQMFARSVAWGVVGLFLAIAPGI
                     VMRSWKRLLIGIAGGLVGGLLGGLLFDPIAKLTENAVVSRLVGLIAIGVLAAVGTGII
                     EKAAKTGWLKVVAGLIAGKQFILYRNPTNIGSSPQCEIYLFKDTQVAPRHAAIHVASG
                     GCELENASPGNATFVNGQAVSRKRLRNGDRIQIGGTVFEYQEKDKR"
BASE COUNT          525 a          759 c          684 g          524 t
ORIGIN      
        1 cgccttcagc gttgacaacg cgtataagcg gaaagcgtcg gaatgccttg ttcaggtgct
       61 cggcatcggc cttgaggcgg cgaatgcgca tgggaagctt tttggccact ggccgacctg
      121 ccgaaccagt cggcgcggaa tctgcctgac gagatgaccc gccaccgaaa gatacgacga
      181 tcttctccgc gggtgtgtct actcgtggct ctggcgttaa ggcggaatct gatcctgcgg
      241 ccttggggat caccacggag tgcccgcacc tcttgcagcg ggcttttctg ccccccagcg
      301 agtccttgac agcaaaggct gtaccgcaat gttgacagtt gaacctaatc attatgtgct
      361 ccacatacaa tccagccggt taatcgagcc ctacggacag tccggccacg aatcctgtca
      421 gggaaacagc cgcaaccagg acgagaaggg caattgactt ccagtgcaag ggcttactgt
      481 atacagttcc actggccccg gactgccctg acaagaggtt cgacttgcgt agtcgcaaca
      541 ctcccagaag gccgacactc aatagcagta acaagcttgg gataccatag gacaccaagc
      601 tgagcaggaa cccgatagcg gaggccgcca gcaagacaag tttccagggg aaaccggcgt
      661 tgaggcttcg ggcagcgtag accgggtcga caatcgctgc cagatccggc atggaagcga
      721 tgggctgcca attgtccatc ccgcctttcc ataccatcgt gcccgtggtt atgcggttct
      781 cctgtgccca cttggccaat tctgtggcag tcacgggccc gtagaactgc gtcccgatgg
      841 cacagtgcca gatcggctga ttgtctgacg catcgactgg actggccatg gacgtgccct
      901 cgatcttcac gtccgggccc tgtcgcagta tgctgacctg agagcagcca taggtcgaac
      961 aaccgaggtt ctcctgccag cactcggcat ggaatggcaa gtggcattcc gagcactgga
     1021 tgacctcatc gtcttcttca atggggcttt ggcaaatgct gcagagcgcc tcgaccgcga
     1081 cctcgtcgcg cgcttcctca gcctgctcac tcttcgtttc gcctggaatg tgcttgggct
     1141 gactggcgac agggtcctgc gtggacacct gcgggatgac gagggatgaa ccgcaggtat
     1201 tgcagcgtcc cctacgaccc gatagtcgcg catcgacacg gaaccgggtt ccgcaggcgc
     1261 agcggaactc gatcttttcg gccctggttg tctgctcgtc gctcatgtct caaccacgct
     1321 tgccatgttg ggtatgttca gaaaccactt accgcttatc cttctcctgg tactcaaaca
     1381 cggtgccacc gatctgtatt ctgtcgccgt tccgtaaacg cttccgggag acagcctgcc
     1441 cgttcacaaa cgtcgcatta ccaggcgaag cgttttcaag ctcgcatccg cccgacgcga
     1501 cgtggatggc ggcatgacgc ggagcgacct gtgtgtcctt gaacaggtat atctcgcatt
     1561 ggggcgagct gcctatgttc gtgggattgc ggtacaggat gaactgcttg cccgctatga
     1621 gccccgccac gaccttgagc cagcccgtct tcgcggcctt ctcgatgatg cctgtaccga
     1681 ccgccgcaag gacaccgatc gcgattagcc ccactagccg gctaaccacc gcattctccg
     1741 tcaacttggc gatggggtca aagagcaacc cgccgagtag ccctcccaca aggccaccgg
     1801 ctatgccaat tagaagtcgc ttccaactcc tcatcacaat gccaggcgca atagcaagga
     1861 ataaccctac tacaccccaa gcgactgaac gggcgaacat ctgcttccct gtactatcgg
     1921 agcctgcgcc aagcgctcga tagagatggt cgacgaatag gccaacgatc acgccgccaa
     1981 tgagcccaag gaagatgcca acgcaactgc ttatgccagc ggaacgccag tttctgctga
     2041 ctactggctc ggcgatagat aaggacatcg ccaatatgag gccgacgaga ccaatgaaca
     2101 taatggattt gagttggctg tgccgcttgt ccttttcgag ctgtctgtcc ctcgccgctt
     2161 ccttctggaa gtcggacata ctgtcatctc gggctatacg aatgtaggga ttatcgtcgt
     2221 attcggcagc gagggcctca agttgtgctt cagcccttac ttcagagata tcgccgtcgc
     2281 gcgcctgtac gaatatcgtg ttggccttgc cgaggaattc catgtattca tctaaggggg
     2341 atgacatgcc ctgatagaca gcctctccca ccccccaagc caccagaccg cccagaaggc
     2401 cgaaaacggc catatagaag aggctgttat accagatgct tgtcttgccc ggcacggtcg
     2461 cttgtattgc ggtaacttgc tgggcggccg tc
//
LOCUS       DAOSAI010000359         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_27402_length_2490_cov_3.150719, whole genome shotgun sequence.
ACCESSION   DAOSAI010000359 DAOSAI010000000
VERSION     DAOSAI010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27402_length_2490_cov_3.150719"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..551)
                     /locus_tag="PLM83_10075"
     CDS             complement(291..551)
                     /locus_tag="PLM83_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOK71788.1"
                     /translation="MEVLKVSAKSNPNSVAGALAGVIRERGGAEVQAIGAGAINQAVK
                     AVAIARGFVAPSGLDLVCIPAFVDVTIDGEERTAIKFIVQPR"
     gene            complement(646..1437)
                     /locus_tag="PLM83_10080"
     CDS             complement(646..1437)
                     /locus_tag="PLM83_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOK71789.1"
                     /translation="MRVAMLGDIIGRPGRRHTLRWIADLRSREELDFVVVNGENAAGG
                     IGLTPDVSLEIFDAGVDVITTGNHVWRNREMAGFLQTEKRVLRPANYPQGVPGNGWTI
                     VTKGALRLAVLNLQGRVFMQPIDCPFICADRAIEQIGSNADAIVVDFHAEATSEKQAL
                     GLYLDGRVSAVVGTHTHVQTSDERVLANGTGFITDLGMCGPVNSVIGMDPDTVIPKFI
                     TAMPSKFEVGKGPVIVCGVIVEIDTSTGKTKYVQRFLEELDDTIR"
     gene            complement(1541..2092)
                     /locus_tag="PLM83_10085"
     CDS             complement(1541..2092)
                     /locus_tag="PLM83_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71790.1"
                     /translation="MKKKVIAVKEVRTKEIDGSSASIGLLVSILVRYPEVCTVSYTPD
                     AKMLGFSFMVSRRLEDDEVEGFRSSVLDSISVYLDLLKFGETPTVEVCAVYLGSITQI
                     QVTRDVATLTGDEISLLIGLIIDAFDDDLVTDRSEDLEEEEMIVQEEFIANLLVDLKE
                     TTQEKRLIGFREGGRVIVYNRPA"
     gene            complement(2178..>2490)
                     /locus_tag="PLM83_10090"
     CDS             complement(2178..>2490)
                     /locus_tag="PLM83_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459753.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOK71791.1"
                     /translation="VLVQAADAVSAARPGARRETLEAYIKRLEKLEQIADSFEGVDKA
                     YAIQAGREIRIMVKPDKIDDLASVKLARDIVKKIEQELEYPGQIKVTVIRETRSVDYA
                     K"
BASE COUNT          559 a          763 c          550 g          618 t
ORIGIN      
        1 cagagtcact gtcccacccc gcaatcctgt caagatcgcg ctcctcgagc ggtcggagca
       61 cggccctttc cagttgttgc atctacgacc ccttcttccg gtacgactcc acctcccaac
      121 gcgcttcttg gattcgcctt gcagcaggta tgctccttca ggaaactgta cccaaggtcg
      181 gccgtcatag ccatcgcgag caacatgtga tataggtata gacaaatatt tattatagcg
      241 tcaaaaacaa gaagcacgcc gttccgaccc tggcggagga aaagcctaga ctaccgcggc
      301 tgcacgataa acttgatagc tgtgcgttct tcaccatcaa tcgtcacgtc cacaaaggca
      361 ggtatgcata ccagatccag cccgctcggt gctacgaatc ctcgagcaat agccactgcc
      421 ttaaccgcct gattaatggc gcccgctccg atagcttgca cctccgcgcc tccgcgctca
      481 cgtatcaccc ctgctagagc tccagccact gaattaggat ttgacttggc agaaaccttt
      541 aggacttcca tgacattatc ctcctggttg gctaaatcag cgtctactcc taactgatgt
      601 attagccctt tcttggaaaa ctccttctgc tcattggcac aagtttcatc gaatggtatc
      661 atcaagttcc tccaagaatc gttgcacata cttcgttttc ccagtggaag tatcgatttc
      721 tactatgacc ccgcacacaa tcaccggccc cttgcccacc tcaaacttgg agggcatggc
      781 agttatgaac ttggggatca ccgtatccgg atccattccg ataaccgagt tgacggggcc
      841 gcacatcccc agatccgtga tgaatccagt cccgttcgca aggacccgct catcagatgt
      901 ctgtacatgc gtatgcgtgc caaccaccgc tgatactctg ccatcaagat agagcccgag
      961 agcctgtttt tcgctggtgg cttcggcgtg aaaatccaca acaatggcgt ctgcgttgga
     1021 gcctatctgc tcaatagctc gatcggcgca gatgaaaggg cagtcaatag gctgcatgaa
     1081 aacgcggccc tgaaggttaa gcacggcgag gcgcaaggcg cccttggtca ctatggtcca
     1141 tccatttccg ggcacacctt gtggatagtt ggccggacgc agcacgcgtt tttcggtctg
     1201 gaggaaccca gccatctccc tgttacgcca gacgtgattt cccgtggtta ttacgtcaac
     1261 ccccgcgtcg aatatctcca gtgacacatc tggtgtaagg ccgatgcctc ctgcggcatt
     1321 ctccccatta acaacaacga aatcgagttc ttcgcgggat ctgagatctg ctatccagcg
     1381 aagcgtgtgc ctccgtccgg gcctgcctat gatatccccg agcattgcaa ctctcaacgg
     1441 cactcctccg tttcttcttt aggacgaata agggcaggtt catctgtaaa cctgcccgcc
     1501 ttattcgaac atattctcaa gaggctcagt ttccgccaga tcacgcgggc cgattgtaca
     1561 ctattacacg gcccccctcg cgaaacccga ttagcctctt ctcttgagtc gtctctttca
     1621 aatcaacaag gagattagcg atgaactcct cctggacgat catctcttcc tcttcgaggt
     1681 cttccgacct gtccgttacg agatcatcat cgaaagcatc tattatgagc ccaatcagaa
     1741 gcgatatctc atcgcctgtg agggttgcaa cgtcccttgt cacctgtatc tgggtgatgc
     1801 ttccaagata tacggcgcac acctctaccg tgggcgtctc cccgaacttc agaaggtcca
     1861 ggtaaacaga gatgctgtca agaacgctac tgcgaaagcc ttcaacctca tcgtcttcaa
     1921 ggcgcctcga caccatgaac gagaacccga gcatctttgc atctggcgtg tacgaaactg
     1981 tgcagacctc tggataccgg acgagtatgg acacgagcag tccaatgctg gccgatgatc
     2041 catcgatttc cttagttcgc acctctttca ccgctatcac cttcttcttc atctctccgc
     2101 tcctgtaacg aacttctctt tcacagaagc gattcctgct gaccatctta cgcgtaccgg
     2161 acttcgatag cccacaatta cttggcgtaa tcgaccgatc ttgtttcgcg tattactgtc
     2221 accttaatct ggccgggata ctccagttcc tgttcgatct ttttcacaat atcccgtgcc
     2281 agcttaaccg atgcaagatc atctatcttg tcgggcttga ccatgattct tatttcccga
     2341 ccggcctgta tcgcgtatgc cttatcgact ccttcgaacg agtcagcaat ttgttccaac
     2401 ttctcaaggc gtttgatata cgcctcaaga gtttcccgcc gcgcgcccgg acgcgcagcc
     2461 gagacagcat cagccgcttg aacgagtacc
//
LOCUS       DAOSAI010000360         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_27612_length_2476_cov_3.424205, whole genome shotgun sequence.
ACCESSION   DAOSAI010000360 DAOSAI010000000
VERSION     DAOSAI010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27612_length_2476_cov_3.424205"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLM83_10095"
     CDS             complement(<1..385)
                     /locus_tag="PLM83_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOK71792.1"
                     /translation="MASKKLIIGAALAFAIILSVASIPTSGFAAGAAAPKALSVPKAE
                     LSDIDGHWAEGAINALVKMGVIAGYPDGTFRPEEPVTRAELSKIVARTFGYAPTVDTK
                     FSDMEGHWAEPFVSALDTAEVVTGYP"
     gene            546..>2476
                     /locus_tag="PLM83_10100"
     CDS             546..>2476
                     /locus_tag="PLM83_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOK71793.1"
                     /translation="MEEHKGRKILIIDGHSLAHRAYYALPATLTRADGTPTNAVLGFC
                     NMVAKMLEEGDFDGGVCAFDRPSPTFRHEQFEDYKATRKPMDDSLRVQMPIIREAAEA
                     FGFTLAELDGWEADDVIGTIARMAEEAGDEAVIVTGDKDALQLVSDNVRVVLTRKGIS
                     KFEEYGPDEVREKYGFGPEFVPDFKGLMGDPSDNLPGVPGVGEKTAMRLVSELGTVEN
                     VLENVHSLKGERLRNNLLEYGDVALESKRLATIDCNAPVGIDIEECCMLRQDPQRLAG
                     FLQKQDFRALIKRLGLSGTGASGENSGGLFAVPAVNVDSGSQAGIGQGPKTEIVSAEE
                     SGRELANRLKDCGRFGLDVIGVGPRAMTARVEGIAFQAEGLSAYVPVGNGEGRLSVEA
                     VADIFGDVLADTDIEKLCHDSKEKIILLRSIGCALSGVTFDSMIASYLVDPGSPNAQL
                     CDVAERWLGVEIPDVREAIEAGAKKARNASYCPEVSEMAVMCGECLRTLPELEATLLA
                     SISSSGMDKLYRDVEMPLAATLAEMEMTGIKVDSKRLGELSREMDGRLDQLVADIYSL
                     AGEEFNVNSTKQLGHVLFDRLGLPPQKKTKTGYSTDAEVLESLANSHPIIQKVVDYRE
                     ISKLKSTYVDALPELVNSKT"
BASE COUNT          566 a          580 c          777 g          553 t
ORIGIN      
        1 cggggtaacc tgttacgact tccgcagtgt caagggcgga tacaaagggt tctgcccagt
       61 gaccttccat gtccgaaaac ttcgtgtcaa cagtgggggc gtatccaaac gtccgggcca
      121 ctattttcga aagttcggcc cttgtcacag gttcctcagg cctgaatgtg ccgtcaggat
      181 acccggcaat tactcccatt tttacaaggg cgtttatggc tccctccgcc cagtgcccgt
      241 ctatgtcgga aagctctgct ttcgggacgg agagtgcctt tggggccgcg gctccagccg
      301 caaagcccga agttggaatc gacgccacgc tcaggataat ggcgaatgcc agcgcagccc
      361 ctataatcag cttcttcgaa gccatgttca ctgctccttt cgtcgaggat taaggtccgt
      421 atggttgtgt tcaccatact actgccatat ccttcaagca gtttgaggca catgaggcct
      481 cagaaggtat ttgcgccata cctgtggaag aatatccgaa gatttagcgg ggaggaataa
      541 tcgttttgga agaacacaag ggacgtaaga tcctgataat agacggtcac agcttggcgc
      601 atagggccta ctacgctctt ccggcgacgc tcacgcgggc agacggaacg cccaccaatg
      661 cggtgcttgg tttttgcaac atggtagcaa aaatgcttga agagggggat ttcgatggcg
      721 gagtatgtgc tttcgatagg ccatcgccta ccttcaggca tgagcagttc gaggattaca
      781 aggcgaccag gaaacctatg gacgactcgc tcagggtgca gatgcccata atccgcgaag
      841 cggcggaggc gtttggattc acccttgctg aactggatgg ctgggaggcg gatgacgtca
      901 ttggaaccat tgcccgcatg gcagaggagg ccggggacga ggcggtcatt gttacagggg
      961 acaaggacgc gcttcagctg gtgtctgaca atgtgagggt agttcttacg cgcaaaggaa
     1021 tatccaaatt tgaggagtat ggccccgatg aggtgaggga gaagtacggg tttggccccg
     1081 agtttgttcc tgatttcaaa gggttgatgg gggacccgtc cgacaatctg ccgggcgtgc
     1141 cgggtgtggg cgagaagacg gctatgcgac tcgtatctga gttgggaacc gtcgagaatg
     1201 tgctggagaa tgtccattcc ttaaagggcg aacgtctgcg caacaacttg ctggaatatg
     1261 gcgatgttgc tttggagagc aagcgtcttg ccaccatcga ctgcaacgct cccgtgggta
     1321 ttgatataga ggaatgctgc atgctcaggc aagatccgca gaggcttgcg gggtttttgc
     1381 aaaaacagga tttccgcgca ctcattaaga ggttggggct ttcagggaca ggcgcgtccg
     1441 gagagaactc cggcgggctt tttgcggtcc cggctgtaaa tgttgacagc ggctcacaag
     1501 ccgggattgg gcaaggccca aagacggaga tcgtcagtgc tgaggagagt gggcgagagc
     1561 ttgcgaatcg cctgaaggat tgtggcagat tcggtcttga tgtgatcggg gtagggccac
     1621 gagcgatgac agcaagggtt gagggcattg cctttcaggc tgagggcctc agcgcatacg
     1681 ttcctgtagg aaatggagaa ggaaggcttt ccgtcgaggc ggtagccgat atcttcggcg
     1741 atgtattggc cgacacggat attgaaaagc tgtgccatga ctcaaaagaa aagatcatcc
     1801 ttctcaggtc gatcgggtgt gcgctttctg gagtcacgtt cgacagcatg atcgcctcgt
     1861 atcttgtgga tccagggtcg cccaatgcgc aattgtgcga tgtggcagag agatggctcg
     1921 gtgtggagat tcctgatgtg agggaggcca tcgaggctgg ggcgaagaag gcgaggaatg
     1981 cgagttactg tcctgaagtt tcggagatgg cagtaatgtg cggcgagtgt ctgaggacgc
     2041 tacccgaact cgaggctacg ctgcttgctt cgatatcctc cagtggtatg gacaagctgt
     2101 accgcgatgt agagatgcct ctcgccgcga ctttggcgga gatggagatg accggaatca
     2161 aggtcgactc gaagcgcctt ggtgagcttt caagggaaat ggatgggagg ctcgaccagt
     2221 tggtggccga tatctattct ttagcgggcg aggagttcaa tgtgaattcc acgaagcaat
     2281 tggggcatgt cctctttgac aggctcggct tgccgccgca gaagaagacc aagaccgggt
     2341 attccacaga tgccgaagtc ctcgaaagcc tcgccaacag tcatccgata atacagaaag
     2401 tcgtagatta cagggagatt tctaagctca agtctacata cgtggacgcg ctcccggagc
     2461 ttgtgaattc gaaaac
//
LOCUS       DAOSAI010000361         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_27621_length_2476_cov_2.363486, whole genome shotgun sequence.
ACCESSION   DAOSAI010000361 DAOSAI010000000
VERSION     DAOSAI010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27621_length_2476_cov_2.363486"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    707..716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <717..930
                     /locus_tag="PLM83_10105"
     CDS             <717..930
                     /locus_tag="PLM83_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71794.1"
                     /translation="RNLRIMFRDEDLQLACTDPDVGRRRWGESCAKVIRRRLDQLRAA
                     ETLEDMRNLWNVRCRGNRMEVWTGRK"
     gene            965..2074
                     /locus_tag="PLM83_10110"
     CDS             965..2074
                     /locus_tag="PLM83_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOK71795.1"
                     /translation="MTEKGIGMYEYVPDVVSPPGSTLLETIEALGMTQAELARRMGRP
                     VKTINEIINGKTAITPKTALQLERVLGIPAHFWRNREEQYQQYLLAQRDEKERAEYSY
                     WLEEIPFRYLVTSGWVKDGANDAERVRLGLEYFGVASVDAWRSTWGSPTAVYRRSPVF
                     EAKPGAVAAWLRKGEIEARTIECSPYRKKAFASALQDIRILTVEQPEVFIPKMQSLCA
                     QTGVAVVFVPEVPESRASGAARWLNRQKALIQLSFRYKTDDQLWFTFFHEAAHVLGGY
                     QSSAFIDNFEPNDLASEVELEADRFAADILMPPTEYRKFTEGIQGGRISRNKVSAFAK
                     SIGVAPGIVVGRLQHDGLLPYTHLNGLKVRLKWPT"
BASE COUNT          587 a          561 c          683 g          635 t
ORIGIN      
        1 aagctcgggg ccttctcttt gataagcgga atccgcacgg tctcttcaca acaagttgaa
       61 ttggcggttt ctctcatatc tgcttttcac acagtctgca cacgccacgc cattatacat
      121 ggattgcgtg gcgcggccga tctcttcaat ggttgttatc aatcccatgt cgtcaagaat
      181 ccacaaggtt cgatagactg tagttctcgt ggacgtggtg atatatggtt tccgcgtcgg
      241 tgcggtcact cgactctgct aattcctcac aagaatcact aatatgtctg gggtgtcaat
      301 ggttgcctcc attcttattg tcccatctgc tgtttgtgga aagcacaatt gaagaagctt
      361 gctcgacttc atgcatgctg gtttagctgc ctgatcccga atcgaactcg gaaaggttag
      421 aggattccag agctaacctt tttgcaatgc agatgctcct cagccatctc ctattagaac
      481 ctgaggcggg tatgagaccc cttccatcgc ggtgatgttc aaactgaatc tgctatggct
      541 attgccagtt ggcgccttaa tctcagaccg atgcagatat ggaggattat ggtgatatac
      601 ctcgaaatac acgttcatgg tcctgggctt gggcgcacaa caaagtgcag aggcacccga
      661 gtccatgaac acgaatctta acctttgggg ttaatatacg atttgannnn nnnnnnacgt
      721 aatttgcgta taatgttcag ggatgaagac ctccaattgg catgcacaga tcctgatgtt
      781 ggcagacgta gatggggaga atcttgtgca aaggtgatca ggcgaagact tgatcagctt
      841 cgggctgcag aaacacttga agacatgagg aatctgtgga atgtccgctg ccgcggaaac
      901 cggatggagg tttggactgg acgaaagtga aggaaattgt cgtaattggg gtggtggact
      961 accattgact gagaagggca taggaatgta cgagtacgtg ccagatgtgg tatcaccgcc
     1021 tggttccacg ctgctggaga cgattgaggc gttggggatg actcaagcag aattggcacg
     1081 gcgaatgggc cgtccggtaa agacgataaa tgagattatc aatgggaaaa ccgcaataac
     1141 tccgaagacg gccttgcagc ttgaacgcgt tttaggcatt cctgcacatt tctggagaaa
     1201 tcgtgaggag cagtatcagc agtatctttt ggcccaaagg gacgaaaaag agcgggctga
     1261 gtatagctat tggctggagg agataccgtt caggtatctt gtgacaagtg gctgggtaaa
     1321 ggatggcgcg aatgatgccg aacgagtcag gcttggcttg gagtatttcg gagtagcgtc
     1381 agtcgatgca tggcgctcga catggggttc gccgaccgca gtttatcgcc ggtcgccggt
     1441 ttttgaggcc aaacccggag cggtggccgc atggctgcgg aagggtgaga tagaagcaag
     1501 gacaatagag tgcagccctt acaggaagaa ggcgtttgct tcggctcttc aggatattcg
     1561 tattctcaca gtggagcagc cagaggtttt cattccgaaa atgcaatcct tgtgtgccca
     1621 aactggtgtg gcagtagttt tcgtgcctga agtgcccgaa agccgcgcat cgggtgctgc
     1681 tcgatggttg aacagacaga aggcccttat tcagctgagc tttcgctaca agaccgacga
     1741 tcaactttgg tttacatttt tccatgaagc agcccacgta ctgggcggct accagagcag
     1801 tgcattcatt gacaatttcg aacccaacga tctcgcatca gaagtagagc ttgaggcgga
     1861 tcgcttcgct gccgacatcc tcatgccgcc gactgagtat cgcaagttta ccgaaggcat
     1921 tcagggaggg cgaataagtc ggaacaaggt ttcagcattt gccaagtcaa tcggcgtggc
     1981 cccgggaatt gttgtcggac gtctgcaaca tgatgggcta ctgccgtaca ctcatcttaa
     2041 cgggctcaaa gtgcgtctga aatggcctac atgatttgag gcttgatccg ttttccggcg
     2101 tatctgctga tgactagatt gtgtcgcatc acagccacac ccctgttggg ttttcgagtt
     2161 tcctctggat cttgagtcta aagacatatt ccatgcgcct attgacgtcc ttgttgggat
     2221 ccctgacata gttctgaatt gcctgagcac agatttctgc atccattttt gttgaatgcc
     2281 tgattgcatc acagacggcc cgtcgtgcca ttgaccacct gctcggaaag cccaatgttg
     2341 atgagacggg gcttaacgaa gtatgccttt agagcggggt attcaagctt aagtttcgtt
     2401 ttgctgatca ggtgtcaggg gcttggtcaa tatacccgtg aaagtggagt gcgctgtcca
     2461 tgcagagcat tgcttc
//
LOCUS       DAOSAI010000362         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_27834_length_2461_cov_3.428096, whole genome shotgun sequence.
ACCESSION   DAOSAI010000362 DAOSAI010000000
VERSION     DAOSAI010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27834_length_2461_cov_3.428096"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLM83_10115"
     CDS             complement(<1..649)
                     /locus_tag="PLM83_10115"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOK71796.1"
                     /translation="MISGMRKQAGTIVAVIAGVLALGMVIPMLFAGSNANAGSARLTV
                     AKVNSVKVSALDLALEFQQAYSRRVQYGGLKPEDIEELRSDALDALIEDALILDAAKK
                     AGYVANQVVVDSAYEADKAYFSDEREFVSALANWGFTKKSYKEYLGRQQIIGSYPSVA
                     KPVEVTEEEIKEEFDKIAVTQPNLDYEESKENIEQLIRYRKETANRQELVDELRKD"
     gene            complement(713..1369)
                     /locus_tag="PLM83_10120"
     CDS             complement(713..1369)
                     /locus_tag="PLM83_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK71797.1"
                     /translation="MADAGVLNVERLVLGPISTNCYIVWREGHEDAVVIDPGAFSPRI
                     QNILKQHGLECRAIINTHGHPDHIGGNGALRDATGAPVYVGALDADMLAEPDDVFHVL
                     ARSGGQGFSLVADHTLEGGNVLRVGEMEFEVLATPGHTPGGISLLLPDDLTLFTGDTL
                     FACGVGRTDFAGGDEEALWRSIFEVIFSLDERIRVYPGHGPDTTIAAEKAWHGRSRIR
                     "
     gene            complement(1410..1859)
                     /gene="dtd"
                     /locus_tag="PLM83_10125"
     CDS             complement(1410..1859)
                     /gene="dtd"
                     /locus_tag="PLM83_10125"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOK71798.1"
                     /translation="MRAVVQRVTEASVSVDGEIVGSTGHGLVVLLGISREDGSEDVEY
                     MVEKIPNLRIFDDDEGKLNLSLLDVGGGMLAISQFTLYGDCRRGRRPSFTDAAAPEHA
                     NVLYEEFVRGVRNMGVPVETGIFRAEMDVNLVNHGPVTILLDSGKAF"
     gene            complement(1948..>2461)
                     /locus_tag="PLM83_10130"
     CDS             complement(1948..>2461)
                     /locus_tag="PLM83_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(p)ppGpp synthetase"
                     /protein_id="HOK71799.1"
                     /translation="PERAADETPKPSPAKKKAGSSHGVRVPGVDNLLVRFARCCNPVP
                     GDPIVGYVTRGRGVSVHRKDCPNAMIIVNAEKDRVVDVYWDTKDGESYPVEIEMEAYD
                     APGLLTGVINTAVEMKAHVSAVTARTLKNRVAVINMTVEIHDVEHMDSVMERLRRISG
                     VQSVRRAAIN"
BASE COUNT          581 a          760 c          533 g          587 t
ORIGIN      
        1 catctttgcg aagctcatcg acgagctcct gcctatttgc cgtctctttg cggtatctga
       61 tcagctgctc aatgttttcc ttcgattcct cataatcgag attaggctga gttaccgcaa
      121 tcttgtcgaa ttcctctttg atctcttcct cggtcacttc gacaggcttt gctacagagg
      181 gataactccc aataatctgc tgtcgaccaa ggtattcctt gtagctcttc ttggtaaacc
      241 cccagtttgc cagagctgac acgaattccc gttcgtctga aaaataggcc ttgtcagcct
      301 cgtaggccga atctaccact acctgattcg ccacataccc tgccttctta gctgcatcga
      361 gtatgagcgc gtcttctatt agggcatcca aagcatcaga ccgcaactct tctatatcct
      421 caggctttag gccgccgtat tgcacacgac gcgagtaggc ctgctggaat tcgagtgcaa
      481 gatcgagcgc tgacaccttt accgaattaa ccttggctac ggtaagcctg gcgctcccgg
      541 cgttggcatt ggaacccgca aaaagcatgg gaattaccat cccaagcgct aaaacacccg
      601 ctatgacggc aacaattgtg ccggcttgct tacgcatccc tgagatcaag ataataaccc
      661 tcccagctaa gctcaatagc ctacgtcatt gtatcgcacg aagattgcag tgtcaacgaa
      721 tgcgtgaccg tccatgccac gccttttcag ctgctatagt tgtgtctggg ccatgccctg
      781 gatatactcg aatcctctcg tcgaggctga atatgacctc aaagatagaa cgccagagag
      841 cctcttcatc accgcctgca aaatctgtcc gtccgactcc gcacgcaaag agagtatctc
      901 ccgtgaaaag cgtcaggtcg tcggggagca acaggctgat tcctcccgga gtatgccctg
      961 gagtcgccaa cacttcgaac tccatctcgc ccacgcgcaa cacatttccg ccctcaagag
     1021 tatggtccgc tacgagcgaa aatccctgtc ctcccgaccg cgccaacacg tgaaaaacat
     1081 cgtctggttc ggctagcatg tccgcatcga gagcgcctac gtatacagga gctcctgtcg
     1141 catcccgtaa cgccccattg cccccaatgt ggtcgggatg cccgtgcgta ttgattattg
     1201 cccggcactc gagaccatgc tgcttcagaa tattctggat gcgtggggaa aaagccccgg
     1261 gatcgatcac tactgcgtcc tcgtggccct cacgccaaac aatgtaacag ttggttgata
     1321 tcggcccaag cacgagcctt tctacgttga gcacacccgc atccgccaaa taaaacccgc
     1381 ctccttgcag tctcagtctg taaccaaatt caaaaggcct tgccgctgtc aagcaaaatc
     1441 gtcacaggcc cgtgattaac aaggtttaca tccatctcag ccctgaatat accagtttca
     1501 accggaacgc ccatgtttct tactcctctg acgaattcct cgtagaggac attcgcatgt
     1561 tcaggcgcag cggcatccgt gaaactgggc cgtcgcccac gtctgcagtc tccgtataat
     1621 gtgaactggg atatggcaag catgcctcct ccaacatcca gaagtgaaag gttgagcttg
     1681 ccttcatcgt cgtcaaaaat cctcaggttc gggatcttct ctaccatgta ttcaacatct
     1741 tccgagccat cctctctcga aatgcccaga agaaccacaa ggccatggcc tgtgcttcca
     1801 acaatctcac catctacact gaccgaagcc tcggtcaccc tctgaaccac agctctcagc
     1861 gataagcacc tccacgttaa accagccacc caatattatc acaactatca gggctatggc
     1921 gacagctcga acatcaaacg acgcccgtca gttgatcgcc gccctacgca cactttggac
     1981 ccctgaaatc cgtctgagcc tctccatcac agaatccatg tgctccacgt catgtatctc
     2041 cacagtcatg tttatcacag ctaccctgtt tttgagggtc ctggcagtaa ctgcgcttac
     2101 atgggccttc atctccacag cggtgttgat tacacctgtg agaagaccgg gagcgtcgta
     2161 agcttccatc tcgatctcga cagggtatga ttcgccgtcc tttgtatccc aatacacatc
     2221 gacaacacgg tctttttcag cattaactat aatcatggca ttaggacaat ctttcctgtg
     2281 aaccgaaacc ccacgaccgc gggttacata tcccactatg ggatcgccgg gcaccgggtt
     2341 gcaacacctt gcgaacctaa ccagcaagtt gtctacccca ggaacccgaa ctccatggga
     2401 agacccggcc tttttcttgg ctggtgaggg cttgggcgtc tcatctgcag cccgctcagg
     2461 a
//
LOCUS       DAOSAI010000363         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_27965_length_2453_cov_3.972477, whole genome shotgun sequence.
ACCESSION   DAOSAI010000363 DAOSAI010000000
VERSION     DAOSAI010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27965_length_2453_cov_3.972477"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLM83_10135"
     CDS             <1..394
                     /locus_tag="PLM83_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOK71800.1"
                     /translation="LGATRIGHGVRAIEDDAVVSLLAEKGILLEICPTSNVHTGIYAS
                     VKEHPLPELIKKGVAVCINTDDPKISGITLVDEYELANEVLGLSESEIEDMNFKAIAA
                     AFADDRQRARVRSILEGVANCHRNNNQN"
     gene            367..1674
                     /locus_tag="PLM83_10140"
     CDS             367..1674
                     /locus_tag="PLM83_10140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK71801.1"
                     /translation="MSSQQQSELEFDPKPEDRSVVRHNIRVNNMHGIWESLALNLYSP
                     FLGLLAIRLGASNFQVALITALPAAVSSVASLAGAAFLRLFERKKSAACWLIMANRAF
                     LLAIAALPLIPQEKQAWVFVGLVALMNVPGAISHIAWQSLVADTIPKEYRGDAFAERS
                     RLVTAVGIIPALLGGYLCDSMSFPIGYQLVFVAAFLVSLVEVSVLSKFREPRRVGNPP
                     AAGGGSLDPEAGRPTLAQVLANRGYVSFTLKAMIFYLGWMMSQPLFTIYYVRVLECGS
                     LWVAVFSVVASIARYLSFPKWSRRAAKKGNAWALAAATAGMALTPVMVAMSPWAWLVA
                     VFNVSMGYFTSGTTLLMLNTLLEMVPEKNRTSFIAYHNSAVNLSSFIGPIVGKTLVDS
                     FDIYWALGISAMIRAAGSIGFFLLARTLMSDRRHAGARGGESF"
     gene            1671..2180
                     /locus_tag="PLM83_10145"
     CDS             1671..2180
                     /locus_tag="PLM83_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71802.1"
                     /translation="MRPKSNVRYYWIFAVVVLAGCALASCWGRNGMSEASAARYAYLP
                     DSLNADADVSVAEFAAVRFTAYYNCPGNLTSRLMRQSARCFLGTNSIDLFIDTVTQAY
                     WNTHVAGSRFSVGDDQVIKAYAEAGDVAMDWLRRFFPGVDDRNMRIIFSIKGYQIGVY
                     SCGEFVISR"
BASE COUNT          529 a          568 c          725 g          631 t
ORIGIN      
        1 attaggggca acaaggatag ggcatggcgt cagagccata gaggatgatg ctgttgtctc
       61 acttttggct gaaaaaggga ttcttctgga gatctgcccc accagcaatg tgcacaccgg
      121 gatttatgct tccgtcaaag agcatccgct gcctgaactc atcaaaaagg gtgttgcagt
      181 gtgtataaac actgatgacc ccaaaatttc cgggatcact ttggtggatg agtatgaact
      241 ggccaacgag gttttggggt tgagcgaatc cgaaatagag gacatgaact ttaaagcaat
      301 cgcggcggct tttgcagatg atcgacaacg cgcaagagtg cgttccattc tggagggtgt
      361 cgcaaattgt catcgcaaca acaatcagaa ctagagtttg acccgaaacc tgaagaccga
      421 agcgtggttc ggcacaacat cagggtcaac aacatgcacg gaatatggga aagcctcgcg
      481 ctcaatctat acagcccatt cctcgggttg cttgcgataa ggctgggcgc gagcaatttt
      541 caggttgctc tgataacggc gcttccggcg gccgtgtctt ccgtagcgag ccttgccggg
      601 gccgcatttc tccggctctt tgaaagaaag aaatctgcgg catgctggct gataatggcc
      661 aatagggcat tcttgctcgc tatcgccgcg cttccgctta tcccgcagga aaaacaggcc
      721 tgggtttttg tgggtttggt ggcattgatg aacgtgccag gcgccatcag ccacatagcg
      781 tggcagagtc tggtggccga tactattccc aaagaataca ggggagatgc gtttgcggag
      841 agaagccgtt tggtgacggc tgtcggcatt atacccgcgc ttctcggcgg gtatctatgc
      901 gattcgatgt ctttccctat cggatatcaa ctggttttcg tggcggcgtt cttggtttcc
      961 ctggtggaag tcagtgtctt gagcaagttc cgcgagccta gaagagtagg caatccgcct
     1021 gcggccggcg gcggatcgct ggatccagag gctggccggc cgacgcttgc acaggttctt
     1081 gccaataggg ggtatgtcag ctttacgctg aaggcgatga ttttctactt gggctggatg
     1141 atgtcgcagc cgctttttac catctactat gttagggtgc ttgagtgcgg aagcctatgg
     1201 gttgccgtat tctcggtagt tgccagcatt gcgcggtact tgtcatttcc taagtggagc
     1261 aggcgtgcag cgaaaaaggg caatgcatgg gcgcttgccg cagccacggc gggaatggcc
     1321 ttgacgcctg tgatggttgc gatgtctcct tgggcgtggc ttgtggcggt cttcaatgtg
     1381 tcgatgggct actttacatc cgggaccacg ctcctcatgc tcaatacttt gttggagatg
     1441 gttccggaga agaacaggac ttcgtttatt gcctatcaca acagtgcagt gaatctttca
     1501 tcattcatcg gacccatcgt tggcaagacc cttgtcgact cgttcgatat ctactgggca
     1561 cttggcatca gcgcgatgat aagggcagcg ggaagcatcg gctttttcct gctggcccgg
     1621 acactcatgt ctgataggcg ccatgcaggc gcgcgaggag gagagtcatt ttgagaccaa
     1681 agtctaatgt gagatactat tggattttcg ctgtggttgt acttgctggg tgtgcgcttg
     1741 caagttgttg gggacggaat gggatgtcag aggcgagtgc tgcaaggtac gcatatctcc
     1801 cagactcgct caatgccgat gccgacgtat cggtagccga gtttgctgca gtcagattca
     1861 ctgcttacta caattgcccc ggaaacctca cgtcaaggct aatgaggcag agtgctcgct
     1921 gtttcttagg aacgaactcg attgacctgt ttattgatac tgttacacag gcctactgga
     1981 atactcatgt agctggttcc aggttctctg ttggcgatga tcaggtaatc aaggcatacg
     2041 cagaagccgg tgatgttgca atggactggc tgaggcggtt tttccccggc gtggatgaca
     2101 ggaatatgag gataatcttc agcatcaagg gatatcagat aggggtgtac agctgcggcg
     2161 aattcgtgat atcgagataa tgcaatggga gtagtctgca tatccgttcc gtcaccatgc
     2221 cccatcaagc atataccagt gcaacgcgct gtggaatatg tctgattgag ctgcttgatc
     2281 accgtggcgg atactacggt ttccggacgg caggagctgt tgatactcct tgttcgcagg
     2341 cgcaagctaa tttggggaag acgcagttcg actcgtgggg atggggattt ttcggcgggt
     2401 tcctttggtt cctaggggtt cggagttcca tttggccttc tagacttcag gtt
//
LOCUS       DAOSAI010000364         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_28511_length_2421_cov_3.155537, whole genome shotgun sequence.
ACCESSION   DAOSAI010000364 DAOSAI010000000
VERSION     DAOSAI010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28511_length_2421_cov_3.155537"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..496)
                     /locus_tag="PLM83_10150"
     CDS             complement(338..496)
                     /locus_tag="PLM83_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71803.1"
                     /translation="MNPFLSLGAIANGVGVESGLIDGAGAAVKIVYKRIRVAGKVGLA
                     MCTLPLLV"
     gene            complement(813..1913)
                     /locus_tag="PLM83_10155"
     CDS             complement(813..1913)
                     /locus_tag="PLM83_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOK71804.1"
                     /translation="MTKTASKWMALILSGVLLAFAVVTCQKAKNRYTKYTDTFFDAFD
                     TLVTVVVYVQSEEEFDSYYQQIRARFLELHRLYDKYNEYEGINNIKTINDNAGIRPVQ
                     VEQEIIDLILFSKDWCARTGGKTNIALGSVLEIWHEYREQGLYDPASAKLPPMEDLLE
                     AAQHIDIDKVIVDAENGTVFLGDSEMSLDVGAVAKGYATEVVAREMMAAGLKSGMISA
                     GGNVRAIGKPLDGVRECWGVGIQNPVESIVADDGVLDVVFVNDASVVSSGDYQRYYVV
                     DGTVYHHLIDPATLMPARYYHAVTVVTEDSGVADFLSTAVFLLPYDQSRSLVENLDGV
                     EALWVMPDGKVEATSGMKRIMRSHGASGTKAM"
     gene            complement(2171..2398)
                     /locus_tag="PLM83_10160"
     CDS             complement(2171..2398)
                     /locus_tag="PLM83_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71805.1"
                     /translation="MGKDCTVVDAIIDTVETKVDFGEDFKAGSDLTAECKTKRGTYGM
                     KKASVIKKEWHRQIVEIEKWMAGKTIADIRL"
BASE COUNT          610 a          712 c          513 g          586 t
ORIGIN      
        1 cattcgtgaa caaattcaag tggcatgctt tcctgcaaaa cactggtcct gtgcagcagc
       61 ctatgtcccg gtatagcaac gctccgcatg ccttcacaca aagcaaccaa cacaggcttt
      121 cgcaggcaac agcggagtcc gacccgcatt aatcgctcca tcctatcaaa tcaacacgca
      181 tcgccgacgc attaaagaaa ggaggaaaac cgccaggagt ctgctcccca agaacaggca
      241 ggtgcagcaa gcatgatgcg ctccccgctg tgaccggaga attcacacac taccacaaaa
      301 tcaaaagctc agcccgtatg caagtaatac taacgtgtca caccaggaga ggaagcgtac
      361 acatggctag cccaactttg cccgcgacgc ggatccgttt gtagacgatt ttcaccgctg
      421 caccagcgcc gtcaatgagc cccgattcca ctcccacacc attagcgatt gcgccaaggc
      481 taagaaaagg attcatgtca ccgcacatgc cggcgcctca ttcgatgaac atattccagt
      541 ccctttctta gttctgtcga tactccttga cctaggcaag attgaactca aacgaagccc
      601 caagtatctg cattgtctcc ctttgattgc gagagtagaa agtgcaaatc gagaacctga
      661 agcaggttga taggaccaag aactgccaaa cattgctggc ggaaaacgga cttcgaacac
      721 ctactggtga gtccatcgag gaattgaagg attacgtggc acattgtgat tctccggcat
      781 gatcaactgt ttcatgccga aaagcccaaa tactacatgg cctttgtccc cgacgcgcca
      841 tggctcctca ttatcctctt catgccgcta gttgcttcca ctttcccatc gggcataacc
      901 cacagagcct ctaccccatc gagattctcg acgagtgatc tgctctgatc gtatggcaag
      961 agaaacactg cggtggacag gaaatctgct accccagagt cctccgttac tactgtaact
     1021 gcatgatagt atcgcgcagg catcagcgtc gccgggtcta tcaagtggtg atagaccgtt
     1081 ccgtccacaa cataatacct ctggtagtcg ccactgctca ctacggatgc atcgttgacg
     1141 aataccacat cgagcacgcc gtcatcggcg acgatggact caacgggatt ctgtattccc
     1201 acgccccagc actcgcgaac gccgtcaagt ggtttcccaa tcgcccgaac gtttccgcct
     1261 gcacttatca tgcccgactt gagcccagca gccatcatct ctctggcaac tacctcagtc
     1321 gcgtaaccct tcgctacagc gcctacatcc aggctcatct cactatcgcc caggaatact
     1381 gtgccgtttt ctgcatctac tatcactttg tctatgtcaa tatgctgcgc agcctcgagc
     1441 agatcttcca tgggcgggag cttcgcgctg gccggatcat acaggccttg ttcccgatat
     1501 tcgtgccaaa tctcgagcac cgaaccgagg gcgatatttg tcttcccgcc tgtgcgtgcg
     1561 caccaatcct tcgaaaagag gataagatcg attatctcct gttctacctg gacaggcctt
     1621 attccagcat tatcatttat cgtctttatg ttgttgatcc cttcgtactc gttatacttg
     1681 tcatacagcc tatgaagctc aaggaatctc gcgcggatct gttgataata cgaatcgaat
     1741 tcctcttcac tctggacata aaccacaact gtcaccagag tatcaaaggc gtcgaagaag
     1801 gtgtcggtat acttggtata tctgtttttg gccttctggc aggtcacaac cgcaaatgcc
     1861 agcaaaacgc ctgataaaat cagtgccatc cactttgaag cagtctttgt catcggcacc
     1921 accactcact tatattcacc agtacactgc ctatttagca cgaaaagact gagagaaacc
     1981 tcccccatct gagatgagag aggtgcgtga tcagccaaat ggatcaagaa gtctgtccta
     2041 cccttatctt gcgctcttgt aggcctttgc aacagtagcg atgtaatctt cgacactcac
     2101 agcaacgtga gaaatgagct caggagcacg tagcaatccg gtataaacgg cctcacgccg
     2161 cttttaacct tcatagcctg atgtctgcaa tagtcttgcc ggccatccac ttctcaatct
     2221 ccacaatctg cctgtgccat tctttcttga tgacagaggc cttcttcatg ccgtatgttc
     2281 ctctcttggt cttgcactca gcagtcagat cagagcctgc cttgaaatct tcaccaaaat
     2341 cgacttttgt ctcaactgta tcgatgatcg catcaacaac tgtgcagtcc ttacccaata
     2401 ccgcttaggc gatggtagtg c
//
LOCUS       DAOSAI010000365         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_28575_length_2417_cov_2.834886, whole genome shotgun sequence.
ACCESSION   DAOSAI010000365 DAOSAI010000000
VERSION     DAOSAI010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28575_length_2417_cov_2.834886"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..556
                     /locus_tag="PLM83_10165"
     CDS             74..556
                     /locus_tag="PLM83_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71806.1"
                     /translation="MKRLLDGFLLLSLLTVVVTTMTGCTFGRARIGITVNPDPLLIDF
                     NKSEIEGTVTLTLDGLGSFTVDEIGLDYIDAQGQRIEGEGLPQGGKLTQSISLVGTMG
                     VVRKTLPLKGVLGAEGVKIRNEWWLPGALHPASCRMTLLDISGRVVGAGSVGLKWHPY
                     "
     gene            680..1156
                     /locus_tag="PLM83_10170"
     CDS             680..1156
                     /locus_tag="PLM83_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOK71807.1"
                     /translation="MKFDGRSIARAGIIAGVYAGLCLLLAPISYRAFQVRVAEALTVL
                     PIVWPEAVIGLACGALIANVAGPLGVFDVVFGTAATAIAAWLTYRFRNSKMAYFFPII
                     VNGIVVGTYVPFLVGMEIHLWTIPATMLLVAAGEGVAVFALGVPLLNALRRTGMIE"
     gene            1273..>2417
                     /gene="mtaB"
                     /locus_tag="PLM83_10175"
     CDS             1273..>2417
                     /gene="mtaB"
                     /locus_tag="PLM83_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOK71808.1"
                     /translation="MRTVAIATLGCKVNQYDSEAIAGEFRRAGFAVVPFEDVADVYVI
                     NTCTVTQMGDKKSRQLIRRANSKNPDALIVAAGCYAQASPEEVAALPGVYVVSGTYDR
                     ARIVEAVLDFEKDSRGLETTGDHDAAAEDTHGSFRPKGANRVPKVLVEATCAFGGYEE
                     LPADGWTERARAYVKIEDGCENFCSYCKVPYVRGPVRSRELERIRREIERFVVQGYKE
                     VVLTGIDMGAYGRDFGGSPDLADVLAAAANVDGVARVRLSSVDPTDVSEKLIDVMAKS
                     DVICPHLHIPLQSGSDKVLSRMNRRYTISHYQDVVAMARERISDLAVTTDVIVGFPGE
                     DEEAFQATCDACQRLGFSRMHVFQYSRRRDTVASGLTRQVSRKEKER"
BASE COUNT          534 a          546 c          768 g          569 t
ORIGIN      
        1 atttcatatc atatccgccc tgcgcctggc ggtttgccgg tgggcgagta cgcggccatg
       61 ggaggcggga ttcatgaaac ggctacttga cgggtttttg ttgctttcgt tgcttacggt
      121 tgtggtcaca acgatgactg ggtgcacttt cggacgggct aggattggga taacagtgaa
      181 tcccgatcca cttttgattg atttcaataa aagtgagatc gagggcactg tcacattgac
      241 gcttgacggc ctagggagct ttactgttga cgaaataggg ctcgactata ttgatgctca
      301 gggccagaga attgagggcg agggccttcc acaaggcgga aagctcactc agtcaatatc
      361 cctcgtcggg accatgggag tggtcaggaa gacattgccc ttaaagggcg tgctcggcgc
      421 agagggcgtg aagatcagga atgaatggtg gcttccagga gctttgcacc ctgcaagctg
      481 cagaatgact cttcttgata tatcgggcag ggtggttgga gcaggatctg tggggttgaa
      541 atggcatccc tattaggcca tgcctcatat caggcatgga tgtggagctc caccggagac
      601 tgaaagcagc atacgagtat tgcgctaggg atgtgcgcag aattaggtac gtgcgatgtt
      661 ggcgaaagga gtagggcaga tgaagtttga tggtagatcc atagcgcgcg ccgggataat
      721 agcaggcgtg tacgcagggt tgtgtctgct tctggcccct atttcataca gagcttttca
      781 ggtaagagtg gctgaggcct taacagtgct tcctattgtc tggccggaag cagtcatagg
      841 cctcgcgtgc ggggcactca ttgcaaatgt cgcaggtccc ctgggcgtgt ttgatgtggt
      901 gttcggtact gccgccacgg ctatagctgc atggctcacc tatcgcttca ggaattccaa
      961 aatggcctac ttctttccga ttatcgtcaa cgggattgtc gttgggacgt acgttccgtt
     1021 cctcgtcggc atggagatac acctctggac gatacctgca accatgcttc tcgtggctgc
     1081 aggcgaggga gttgccgttt ttgctcttgg tgtgccgctt ctgaatgctc ttcggagaac
     1141 cggcatgata gaatagaaca acagggtagg actcgcttag cgcacgactc ttgtaggcaa
     1201 cggaggcgtc aagtgggcca gtcttcggca ggagaccgga catgccatgc acatttggga
     1261 ggtatctttc gggtgcggac agtcgccata gcaacacttg ggtgcaaggt gaatcaatac
     1321 gattccgaag cgatcgcagg ggaattcagg agagccggat ttgcggttgt gcccttcgaa
     1381 gatgtcgccg acgtgtatgt gataaacacg tgcactgtta ctcagatggg cgataagaaa
     1441 tcgaggcagc tcatacggag ggccaactcg aagaatccgg atgctctgat agttgctgcg
     1501 ggctgctatg cgcaagcttc gcccgaagag gttgcggcgc ttcctggggt atacgtggtc
     1561 tctggaacat atgaccgagc gagaatcgtc gaagccgtgc ttgatttcga aaaggattcc
     1621 agagggctgg agacgacagg ggatcatgat gcagcggcgg aagacaccca cggttcattt
     1681 agacccaaag gggcgaatcg agtcccaaag gtgctcgtgg aagctacatg tgctttcgga
     1741 ggctatgaag agcttccggc tgacgggtgg accgaacgtg ctcgtgcgta tgtgaagatt
     1801 gaggatggat gcgagaattt ttgctcctac tgcaaggttc cttacgtgag agggcctgtg
     1861 cgtagcaggg aactggagag gattcgcagg gagatcgagc gcttcgttgt tcaaggatac
     1921 aaggaagtgg tgcttacggg gatagacatg ggtgcatatg gtcgggattt tgggggatcc
     1981 cccgaccttg cggatgttct tgcggcggct gcgaacgtgg acggcgtagc cagagtccgc
     2041 ttgagctcgg tggatccaac tgatgtgagc gaaaaactga tcgatgtgat ggctaaaagc
     2101 gatgtcatct gtccccatct gcatattccg ctccagtcag gcagcgacaa ggtgctttca
     2161 cggatgaatc gccgctacac tatctcgcat taccaggatg tagttgcgat ggcgagagag
     2221 cgcatatccg acctagcagt tacgactgac gtcatagttg gcttccctgg cgaggacgaa
     2281 gaggcttttc aggctacttg cgatgcctgc cagcgactgg ggttttcgag aatgcacgta
     2341 tttcagtatt cgcgtcgcag agacactgtc gcttccggcc tgaccaggca ggtctcgagg
     2401 aaggagaagg agaggag
//
LOCUS       DAOSAI010000366         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_28815_length_2403_cov_4.117547, whole genome shotgun sequence.
ACCESSION   DAOSAI010000366 DAOSAI010000000
VERSION     DAOSAI010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28815_length_2403_cov_4.117547"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PLM83_10180"
     CDS             complement(<1..1635)
                     /locus_tag="PLM83_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71809.1"
                     /translation="MKWSESKGKRALNASICALALAVVFAFSCHWPANAAGIDRTYLF
                     GMDTEEEIAKFDNDNTDARLELTREVVRQGFGAMKVTPNGKALETKVAVDIDGVTLNE
                     WIGKDALAVHVYIPEDMAIAPNSFFLGMADLSDGWAWVDGVFPKVEVVPGWNDVVYKL
                     SDGMKKVKEGGHYKLYFSFFAVDEGGAKIPLTESFFLDGIWLETLDGDASASSGAPVG
                     DWLWAMDDEAELAGYENDNTGAKFELDAGIVCQGLRSVAVIPDGKAIETKLALDLTGE
                     RLERWIGEHEVVLNAYLPPENEFNPTMFFLGMADLSNGWDWVGGIFAKVEPGPGWNRI
                     TYPLPDEMSNLKPDGKYKLYFAWAHVDEAGKKLPLREKFYIDGIGLAEARVSRDDLIQ
                     RIPEATRLEVAAMLELDDEELIEAVARKTFDYFWREANPANGLIRDRSTKDSPCSIAA
                     VGFGLSAIPVGIERGWITRDEGYERVLVTLRTFASGGVEGKNGFYYHFVDMSEGRRFG
                     DCEISSIDTAIFLTGALTAGQYFAGTEAEALANKVYA"
     gene            complement(1649..>2403)
                     /locus_tag="PLM83_10185"
     CDS             complement(1649..>2403)
                     /locus_tag="PLM83_10185"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HOK71810.1"
                     /translation="ICDDGTLIGDPGCKYGKIFLNAQVWAIMSGIATGKRAESAWRAV
                     VKHLSVDSGPVLFAPAYGESDERIGYLTRYAPGVRENGGRYTHAATWAMIAAAIMGDS
                     SEAWQMYSRICPVRCGSSPDVYKAEPYVTPGNVAGPDSDRYCEGGWTWYTGSATWLFR
                     VGSEWLLGVRPEWDGLRIDPCIPAHWDGFSMRRNFRGATYEIHVKNPDHVSSGVFRMI
                     MDGRKIDGCLLPIVGDGRVHRVEITLGKSNKD"
BASE COUNT          538 a          770 c          556 g          539 t
ORIGIN      
        1 cgcgtaaacc ttgttcgcca aagcttctgc ctcggttcct gcaaaatact gtccggcggt
       61 aagagcaccc gtaagaaaga tcgccgtgtc tatcgaagag atctcgcagt ctccgaaccg
      121 tcgtccttcg gacatgtcga cgaagtggta gtagaatccg ttcttgccct caacccctcc
      181 actggcgaac gttctcaagg tgacgagcac gcgttcatat ccttcatccc tggtgatcca
      241 acctcgctct atccccactg gaatcgccga caagccaaag cctacagccg caatgctgca
      301 cggcgaatcc ttggtgcttc tgtcccgaat cagcccgtta gccgggtttg cctcgcgcca
      361 gaaataatcg aaggtcttcc gcgctaccgc ctctataagc tcttcatcat ccagttctag
      421 cattgctgct acttccaacc tcgttgcctc gggaatccgc tggataaggt catctcggga
      481 gactctggcc tctgcgagcc ctatgccgtc tatgtagaac ttctccctca gcggaagctt
      541 cttccctgcc tcatctacgt gagcccaggc aaaatagagc ttgtatttgc catcaggttt
      601 gaggttgctc atttcatcgg gaagaggata ggttatgcga ttccaacccg ggcccggttc
      661 gaccttggca aatatgcccc ccacccaatc ccaaccattt gagagatccg ccatgccaag
      721 gaagaacatg gtgggattga attcgttctc tggaggcagg taagcattga gaaccacttc
      781 atgctcgcca atccatctct cgagcctctc tccggtcagg tcgagagcta gctttgtttc
      841 gatagccttt ccgtcaggaa tgaccgcaac agaacggagt ccctggcaga ctatccctgc
      901 atctagttcg aacttggccc cagtgttatc gttctcgtat cctgccaact cagcctcgtc
      961 atccatagcc caaagccaat cccccacggg agctcccgac gaagcggaag catcaccatc
     1021 gagggtctcc agccagatcc cgtccaagaa gaacgactct gtcagcggaa tcttggcgcc
     1081 cccttcgtcg acggcaaaga aggagaaata cagcttgtaa tggccgcctt ccttgacctt
     1141 cttcatcccg tcagaaagct tgtagactac gtcattccaa cctggcacaa cctccacttt
     1201 gggaaagact ccgtccaccc acgcccatcc atccgacaga tccgccatgc ccaagaagaa
     1261 agaattgggc gcaatcgcca tgtcctctgg gatgtaaacg tggaccgcca gcgcatcttt
     1321 gcctatccac tcgttgagcg tcactccgtc gatatcgaca gcgaccttag tctccagagc
     1381 cttgccgttg ggagtcactt tcatagcccc aaaaccctgt ctcacgactt cccgagtgag
     1441 ctcaagcctg gcatcggtat tgtcattatc gaactttgcg atctcctctt cagtgtccat
     1501 tccaaacaga tatgtccgat cgatcccggc tgcgttcgcc ggccagtgac atgaaaaggc
     1561 gaacacgaca gccaatgcaa gagcgcagat cgacgcattc aacgcgcgct tgcccttact
     1621 ctcagaccac ttcatacgag ccgcctccct aatccttatt tgactttcca agggtgatct
     1681 cgacacgatg aaccctgccg tcgcctacaa tcggcaacag gcagccgtcg atcttgcgtc
     1741 cgtccatgat catcctaaag actccggacg aaacgtgatc ggggtttttc acgtgaatct
     1801 catacgtagc gcccctgaaa ttccgtctca tggagaaccc gtcccaatgc gcgggaatac
     1861 aagggtcgat tctgagccca tcccattcgg gacggacgcc gaggagccac tctgagccta
     1921 cacggaacag ccatgttgcc gaaccagtgt accaggtcca tcccccctcg cagtacctat
     1981 ctgagtccgg cccggcaaca ttgcctggag taacgtacgg ttccgccttg tacacatctg
     2041 gcgacgatcc gcacctcacc gggcagatcc tgctatacat ctgccatgcc tccgatgaat
     2101 ctcccataat agccgcagct atcatggccc acgtggcagc atgggtataa cggccgccgt
     2161 tctcccgaac tccaggcgca tacctggtca agtagcctat tctctcgtcc gattcgccgt
     2221 aggctggcgc aaaaagcaca gggccggaat cgactgaaag gtgtttgacc actgcccgcc
     2281 aggcagactc ggcgcgtttg cctgtagcaa taccactcat tatcgcccag acctgggcat
     2341 tcaagaatat cttcccgtat ttgcaaccgg gatcgcctat gagcgtgccg tcatcgcata
     2401 tgg
//
LOCUS       DAOSAI010000367         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_28919_length_2397_cov_3.298463, whole genome shotgun sequence.
ACCESSION   DAOSAI010000367 DAOSAI010000000
VERSION     DAOSAI010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28919_length_2397_cov_3.298463"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLM83_10190"
     CDS             <1..814
                     /locus_tag="PLM83_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOK71811.1"
                     /translation="SSNGIPPEVIDEYGITVVPIQVQFGAESFKEGVDLPCDKFYRLI
                     EEPVLPTTSQPAPGDFVNAYRSLVGQCDEILSIHLSSKASGTCQSARLAAEMIEGVKI
                     TIVDTQTASMGTGLLTIAAAEAAKEGKSSDEIIDLMHRGADGTHVFVALPTLKYLRRS
                     GRVPQVQAWVASLLSINPILTTHDGVVEAVERVRTFRGSVRRMIELAVEACGGRAKRV
                     VLMHANAIEDAQGLLDEVQSRISFERIYFSEMGGSMAVHGGAGMLGIAFSQV"
     gene            939..1013
                     /locus_tag="PLM83_10195"
     tRNA            939..1013
                     /locus_tag="PLM83_10195"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:972..974,aa:Arg,seq:ccg)
     gene            1291..2124
                     /gene="spo0A"
                     /locus_tag="PLM83_10200"
     CDS             1291..2124
                     /gene="spo0A"
                     /locus_tag="PLM83_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOK71812.1"
                     /translation="MIGRKTRVLVVENNKDLCEVIEAMLKEEDDIELAGTAYDGIQAL
                     EMIPKTQPDVILLDIIMPYLDGLEVLARLDETGVEERPKIVMLSAFEEEEMVRRCARL
                     GADYYLLKPFDRDNLINRIRQVVSAEPLAPKPKTDRAYASRERAREVDRKPRGAQFDL
                     ETETTRILYNLGVPTYFKGYSYIKEAIMLVTRDIGLLGAVTKSLYPKVAEKHGTTPLI
                     VEAAIRYTIQKTWKQGNPEYINKIFGYAVNLREGKPPTNSFFIAKIAEELRLEMLECA
                     Q"
BASE COUNT          593 a          588 c          673 g          543 t
ORIGIN      
        1 cagctccaat ggcataccac ctgaggtaat cgatgaatac gggataaccg tagtgccgat
       61 ccaggtccag ttcggcgccg agtcattcaa ggaaggcgtg gatcttccgt gcgataagtt
      121 ttacaggctt attgaagaac ctgtgctgcc cactacatcg caacccgcac cgggtgattt
      181 tgtcaatgca tacagaagct tagtggggca atgcgacgag attctttcga ttcacctatc
      241 atcaaaagcg agcggcacgt gtcaatctgc gaggctggcg gctgagatga tcgagggcgt
      301 caagataacc atagttgaca cacagactgc aagcatgggc acgggccttc tgacaatagc
      361 tgcagccgag gctgccaaag aaggaaaaag ctcagatgag atcattgacc tcatgcacag
      421 gggcgcagat gggacacatg tgtttgttgc ccttccaacc ttgaaatact tgcgccgcag
      481 cggcagggtt ccgcaggtgc aggcttgggt tgcatcactc ctttcgatca accccatact
      541 tactacgcat gacggcgtgg tcgaagccgt cgaacgagtt cggacattcc gcggttcggt
      601 aaggcgaatg atagagcttg cagtggaggc ctgtggtggc cgcgcaaaaa gggtcgtgtt
      661 gatgcatgct aacgcgatcg aggatgccca aggcttgctg gatgaagtgc aatcaaggat
      721 ttccttcgag cggatctact tctctgaaat gggaggatct atggcagtac acgggggcgc
      781 agggatgttg ggaatagctt tttcgcaagt ataggggtcc ttaaggcacg cgcggaagtt
      841 gtgcgatgcc ttcagctaga aatggtgatg ttctcggtgc cggacatcat ttgcgaactt
      901 cgccgtttag gcgtataata cactcgaaca gttggaacgc gcctatagct cagtggatag
      961 agcactggcc tccggagcca gttgcgcagg ttcaattcct gctaggcgcg ccatttattt
     1021 gagtccctct gcagatcggc agagggctat tattttcgtg gagtcggcca tatttcacgg
     1081 cgttcccgga gaagttggtg agtagctcta cagttttcga gatattccgt aacattacga
     1141 aggcaatttc agatggcgtg tcgatgaata caacataatc gcgtgtcaat tatgtccaat
     1201 tgagcaggga ctcgcattcc cctcgtagaa tggtccattc gcgttaattt tacgcgcttc
     1261 aaacaactac ggcaataggg gggaagaagc ttgattggga gaaaaactag ggttctagtc
     1321 gtcgagaaca acaaggacct gtgcgaagtc atcgaggcaa tgctgaaaga agaggacgac
     1381 atagaactgg ccggaacggc ctatgacggg atccaggctt tggagatgat acccaagacc
     1441 caaccggacg ttatcctcct cgacatcatc atgccgtatc tcgatggact ggaggttttg
     1501 gcccgcttgg atgaaacggg cgtcgaggaa cgcccaaaga tcgttatgct ttcggcattt
     1561 gaagaggaag agatggtcag gagatgtgct cgtctcggag cggattacta tcttcttaaa
     1621 ccttttgata gggacaacct tatcaaccgg atacggcagg tagtcagcgc tgaaccgctt
     1681 gccccaaaac ccaagacgga tagggcttac gcctctcgag agagggcgcg tgaagttgac
     1741 cggaagccca gaggcgcgca gttcgatctg gaaacagaga ctacaaggat tctttataac
     1801 ttgggtgtgc ccacctactt caaaggctac tcctacataa aagaagcaat aatgctcgtc
     1861 acacgcgaca tcgggctctt gggcgcggtc acgaagtcgc tctatccgaa agttgccgaa
     1921 aagcacggca ccactcccct catagtcgag gccgctataa ggtacacaat acaaaagaca
     1981 tggaaacagg gaaaccctga atacatcaac aagatctttg gctatgccgt gaacctacga
     2041 gaaggaaaac cgccgaccaa ctcgtttttc attgccaaaa tcgcagagga gctccgcttg
     2101 gaaatgcttg agtgcgcgca gtaagtctgc tatgcaatga aatgggcgaa cccgcggcgg
     2161 gccttcggtt cgccgctgtt tgctttgcat acgcagcccg ggtagatcca cggcaacgcg
     2221 agtccgcaga cttccttctg ttgtgcctgc tgtgacttct tctatcgtgt caaccgactg
     2281 agaaaatgtc acccctgtgg ttgtgttccg ctggcaaccc gtgcattatg ctcaggctcg
     2341 cggccgaggc tgagtcttcg atcgccggtg tggagttgaa ctcggctagg ctgtagc
//
LOCUS       DAOSAI010000368         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_28976_length_2393_cov_3.779299, whole genome shotgun sequence.
ACCESSION   DAOSAI010000368 DAOSAI010000000
VERSION     DAOSAI010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28976_length_2393_cov_3.779299"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2393)
                     /locus_tag="PLM83_10205"
     CDS             complement(<1..>2393)
                     /locus_tag="PLM83_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71813.1"
                     /translation="KPRFIVDGCHAGILFGDVGYEELDDPRMAMEKLFALLDLALSHD
                     AGFSLDWGMSTLEEVSKLLPDDIERLRAVIESTDRIEIVNGSYSQPFPMTIGWESNIR
                     QFEEGLRCADAILGVRPRTYVAQEFGHHPQMPQILKGYGFDGCALRVRLNGEVPSCED
                     PIVWWEGDDGTAILCLTTLESVPTCDECAGLFYRSLASGFASLEMSDADFGAFNNLED
                     VVYPVLDRTEAIICPNRDDILGKFSTYSEVIDAVRDMPIRRAGFASASFRPSVCQNAQ
                     LFGAIRAAEAILIVTEILDVYAHILGIELELDQIISDSWRMLLAAENHDSFVVPDHIP
                     GTYTVERSAIFGSYHGLRSNKTVGEKGLELAKDATHACTVAVDRLKVAVGAEINRGIG
                     GSIDAFDLSEQMWHSLALLDVSGVKGKKSTIGRLDFPLGEMTNFRIEFPGTEAEWQVV
                     DEDRWDDGSLRSADVAVQADIPALGWLVARVSPEDEASEAAQNDAIASITDGAAVIDT
                     GLIGAKVTLDPILRVDLQDGSSGEELACIDITRPGSGGDCKVVATGPVMGEIRVDGGE
                     NNEFGTVSCSAWKNEPSVRVECTQAAQHEYVIRPNHRINRVLADYPFGIGEVTPGVFH
                     ALNVVVIETEETNLCIVHAGDPYFEYELEENLVRYAPRCGPSSIRVFVLHDCDDPLEI
                     SRSCMLKPLMVQPPHISSSSDRATKTRGEGDGKAFAGRLIDIRGDVIITSLRRTQIDE
                     VEIRLFNSGREDRQFSLGRLPGYGAWQETRLDGMAVKSGCRPCLCGSDGWVLPPGRIA
                     "
BASE COUNT          548 a          732 c          543 g          570 t
ORIGIN      
        1 gcaatccgtc caggaggcaa cacccagccg tcactcccgc acaaacaagg ccggcagccg
       61 gattttactg ccataccatc caacctcgtt tcctgccagg cgccataccc cggaagtctt
      121 ccgaggctaa actgccggtc ctctctgccg gagttgaaga gcctgatctc tacctcatcg
      181 atctgcgtcc gcctcagtga ggtgatgatt acgtcgcctc gaatgtcgat gagtctcccg
      241 gcgaaggcct tgccgtctcc ctctccacga gtttttgttg ctctatcaga gctgctacta
      301 atatgcggag gttgcaccat taatggtttg agcatgcagg atcttgaaat ctccaagggg
      361 tcatcgcaat catgaagcac aaacacccgt atactggatg gtccgcacct tggggcatac
      421 ctgaccaggt tttcttcaag ttcatactcg aaataggggt ctccagcatg cacaatacag
      481 agatttgttt cctcggtttc gatcacaacc acattcaggg catggaacac acccggcgta
      541 acctccccta ttccaaaggg gtaatctgca agcacccggt ttattctgtg gttcgggcgt
      601 atgacgtact cgtgctgtgc cgcctgggta cactcgacac gcactgaagg ttcattcttc
      661 caagcagagc acgatacggt cccgaattcg ttgttttctc ctccatcaac ccgaatctcc
      721 cccatcacgg ggccagtagc aactaccttg caatcgccgc cactgcccgg cctagtgata
      781 tctatgcaag ccaattcctc acccgaagac ccatcttgca gatcaactct gaggattgga
      841 tccaatgtca cctttgcgcc tattaggccg gtatcgatga cagcagcgcc gtccgtgatg
      901 cttgcgatcg cgtcattttg ggcagcctca gaagcctcat cttccgggct aaccctggca
      961 acgagccagc ccagcgccgg aatgtcggct tgcacagcaa catcagcact ccgaagactt
     1021 ccatcgtccc atctatcttc atcaactacc tgccattccg cttcagttcc aggaaactct
     1081 atgcggaaat tcgtcatctc tccgaggggg aaatccagcc tccctattgt cgactttttc
     1141 cccttcactc cgctcacatc caatagcgcc aagctatgcc acatttgttc actcagatcg
     1201 aaggcatcga tactacctcc aatccctcgg ttgatctctg cgcctacggc taccttcagg
     1261 cgatcaacag caacggtgca tgcatgggtt gcatcctttg ccagctccaa gcccttttcg
     1321 ccaaccgtct tgttggatct cagtccatgg tatgacccga atatagctga cctttccaca
     1381 gtgtaagttc caggtatatg gtcgggcacc acaaacgaat catggttttc ggccgcgagc
     1441 agcattctcc atgaatcact gatgatctgg tctagctcta actctatgcc gagtatgtgg
     1501 gcgtacacgt ccaggatctc ggtaacaatg aggatcgcct cagcggccct tatcgcgcca
     1561 aagagctgtg cattttggca gactgacggg cggaacgatg cgcttgcgaa acccgcacgg
     1621 cgaataggca tgtctcttac ggcatcgatg acctctgagt aggtgctgaa tttccccaat
     1681 atgtcgtctc tattagggca aatgatcgcc tctgtccgat cgaggacagg gtatacaacg
     1741 tcttccaggt tgttgaaggc tccgaaatct gcatcgctca tctccagcga cgcaaaccca
     1801 gaagcaaggc tcctgtagaa cagtcccgcg cattcgtcgc aagtaggaac tgactcaagt
     1861 gtagtcaagc agagtattgc ggttccatca tcaccctccc accagactat tgggtcttcg
     1921 cacgatggaa cttcgccatt tagcctgacc cgcaacgcgc acccgtcaaa cccatatcct
     1981 ttgagaatct gaggcatctg gggatgatga ccgaactcct gcgcaacata tgttctgggc
     2041 cttacgccta aaatcgcgtc ggcacatctc agtccctctt caaattgacg aatattcgac
     2101 tcccacccta tagtcatcgg gaacggctgc gagtaggagc cgttcactat ctcaatccta
     2161 tcagtagact ctattactgc ccgaagcctc tctatatcgt caggcaagag cttcgaaacc
     2221 tcttccaagg tggacatgcc ccagtccagc gagaaccctg catcatggct gagcgcgaga
     2281 tctagcagcg caaagagctt ttccatcgcc atacggggat catcgagttc ttcgtatccg
     2341 acgtctccaa aaaggatgcc tgcgtgacac ccatccacga taaacctggg ctt
//
LOCUS       DAOSAI010000369         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_28995_length_2392_cov_4.566966, whole genome shotgun sequence.
ACCESSION   DAOSAI010000369 DAOSAI010000000
VERSION     DAOSAI010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28995_length_2392_cov_4.566966"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLM83_10210"
     CDS             <1..298
                     /locus_tag="PLM83_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71814.1"
                     /translation="FEFHATGHNNSEDAFRAQVLFDGSVVTEVLLPSPVEPQYSYFSA
                     YVPCVPPGVTVDILFIKPGAGTWFIDDVGFIAEGPCTGGYWHPFGGCDSGVSTV"
     gene            474..911
                     /locus_tag="PLM83_10215"
     CDS             474..911
                     /locus_tag="PLM83_10215"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOK71815.1"
                     /translation="MAKTTFSASMAWSGSGMYCEGGTRGFKVEVDEPKALGGTDRAMN
                     PVELLLCALGGCMSICAAAFAGACGVDLQGFSVEVEGDLDPDGFLGKNPNVRTGYQGI
                     RYRMIIDSPSPQENIDRLVETIEKRCPISDTLRGVDVVRSAST"
     gene            1060..1527
                     /locus_tag="PLM83_10220"
     CDS             1060..1527
                     /locus_tag="PLM83_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOK71816.1"
                     /translation="MRELDLHGMKTADAMSFFMREYDNALESGSSSLKVIHGYGSGGV
                     GGDTRRCIRALLSANPACVDYVAGEDVDGNPGYTVVYPKRRLPSGATGLYDSIIQFCS
                     SPKTKSAIVRRFVRRTPESEIDRALRDLENRGRLRTFFRNGRKHYIDSRSGRV"
     gene            1664..2053
                     /locus_tag="PLM83_10225"
     CDS             1664..2053
                     /locus_tag="PLM83_10225"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOK71817.1"
                     /translation="MGGRIKVIGVFEPEEDWHLEDEDGQRLEGALSPVAHLVASLVGC
                     SGLTMRGVMDKMKIEHQGIVIEGIAAISDERPTFVRSIWLEVSAPGACINEAQFASIL
                     EMTEKYCLIAQTFRHGPELILSAKTIP"
     gene            2070..2339
                     /locus_tag="PLM83_10230"
     CDS             2070..2339
                     /locus_tag="PLM83_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOK71818.1"
                     /translation="MNDEQKRDVINRLKRIEGQVKGVQRMIEEDKCCADTLDQIAAAR
                     AALYNAGVLLLERHARDCVMEAMAEGGEGEVIDELVRVIKKFARS"
BASE COUNT          563 a          560 c          746 g          523 t
ORIGIN      
        1 ctttgaattc catgccaccg gtcacaacaa ttcggaggat gcctttcgcg cccaggtgct
       61 cttcgacggc agcgtggtga cggaggtgtt gcttccgagc ccagtggagc ctcagtacag
      121 ctactttagt gcctatgtac cctgcgttcc tcctggcgtc actgtagaca ttctgttcat
      181 caagcctggc gcagggactt ggtttatcga tgatgtcggg ttcatagccg agggcccgtg
      241 caccggcgga tactggcatc cttttggcgg ttgtgattct ggcgtgtcca cagtatagcc
      301 tgaacgcaaa atggaagcta actgaagggc aagcgccaac aagagacagg cattcggcat
      361 cgatctgccg actttctgaa gggcgcgtgc ccttcgatga catggccgaa tacactatac
      421 acgtatgtgg tatactacaa aaaaagagat cagtccggga ggtcatgaaa taggtggcca
      481 aaactacgtt cagcgcgtcc atggcgtggt caggcagtgg catgtactgc gagggcggaa
      541 cgagagggtt caaagtcgaa gtggacgagc cgaaggcgct cggcggaact gaccgggcca
      601 tgaatccggt ggaactcctt ctatgcgcgt taggtgggtg catgtcgata tgcgctgctg
      661 cctttgccgg ggcgtgcgga gttgatctgc agggcttttc cgtagaggtc gaaggagacc
      721 tggatccaga cggatttctc ggtaagaacc ccaatgtgcg caccggatat caggggatac
      781 ggtataggat gatcatcgac tctccatcac cccaagagaa tatcgacagg ctggttgaga
      841 cgatcgagaa aaggtgtccc atctccgaca ctctgcgtgg agtcgacgtt gtccgcagtg
      901 caagcacgtg atgcagcagt agagtgcgcg tccttctgcc ctttgttttg caggagggcg
      961 cgcgtccgca attgggtggc gggctgaacg tctgtgttag ccgtgaggca ccatgccccg
     1021 gatgatcggc gttagcagat gttttggagg cgaatatcct tgcgcgaact ggacctgcac
     1081 ggcatgaaga ccgcagatgc catgtcattt tttatgagag aatacgataa tgcccttgag
     1141 tcgggttcat ccagtctaaa ggtgattcat gggtacggat caggaggggt cggaggagat
     1201 acccgccgtt gcataagagc gcttctgtct gcaaaccctg cgtgcgttga ctacgttgca
     1261 ggtgaagatg ttgatggaaa tcccgggtat acagtcgtct accctaagag gcggttgccg
     1321 tcaggtgcta cgggactcta cgattccatc atacagttct gttcatcacc taagacaaag
     1381 tcggcgatcg taaggcgttt tgtgagaagg acaccggaga gcgaaataga cagggccttg
     1441 agagatctcg aaaacagggg gagattgcgg acgtttttca gaaatggcag aaaacactat
     1501 atagattccc gatcgggccg cgtgtgaggt tctaaggcgc agcctggcag agaactcatc
     1561 tttcgcaatg tggcgtactg tgtgggctga gcaaatgcat gagccggacg tgaactcgca
     1621 tgaggagttt cacactttcg acattgaaag gcggcattga ttgatgggag gcagaatcaa
     1681 agttatcggc gtgtttgagc cagaagagga ctggcatctg gaggacgaag atggccagcg
     1741 ccttgagggc gcgctcagtc ctgttgcgca tcttgttgcc agtttagtgg ggtgctcggg
     1801 actgactatg cgcggggtca tggacaagat gaaaatagag catcagggga tcgtgatcga
     1861 gggaatagcg gccatctctg acgagcgtcc cacatttgtg cggtccattt ggctggaggt
     1921 gtccgccccg ggcgcctgca ttaatgaagc tcaattcgcc agcatcctgg agatgactga
     1981 gaaatactgc ctgatcgctc agacattcag acacgggcct gagctgatcc tatcagcgaa
     2041 aacaataccc taataggagg attgctggag tgaacgatga gcagaagcgt gacgtcatta
     2101 accggcttaa acgaatagag ggccaggtaa aaggcgtaca acgcatgata gaagaggaca
     2161 aatgctgtgc tgacacactg gaccaaatcg ctgccgcgcg cgctgctctg tacaatgcag
     2221 gcgttctgtt actcgagagg catgcgcggg attgcgttat ggaggcgatg gccgaaggcg
     2281 gagaaggcga ggtcatcgat gagcttgtgc gcgtgatcaa gaagtttgcc aggtcgtgaa
     2341 ggccgaagaa ctgtccgaga caaaaatgcc gaccgggctg acgctgccat gg
//
LOCUS       DAOSAI010000370         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_29034_length_2390_cov_3.989722, whole genome shotgun sequence.
ACCESSION   DAOSAI010000370 DAOSAI010000000
VERSION     DAOSAI010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29034_length_2390_cov_3.989722"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLM83_10235"
     CDS             complement(<1..668)
                     /locus_tag="PLM83_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOK71819.1"
                     /translation="MERYARIIGTGLYLPETEISNAHMAERINQAAPGLGDVVFKFEE
                     SSRIMKRYYAPRDWATSDLAAKAAMAALEDAGVAPEDLDLIILGTDSPDFITPATSVV
                     TQHKIGAKNAGTYDIGCACASFPTGIAAAAGHIATNPWIRYVLVIGAYMMSKLADPND
                     VTSFFYGDGAGAAVLTGDDKPGFITSSFIADGSYHEAWGIYSGGTYEPATCDSVQAGR
                     TNVR"
     gene            complement(708..2000)
                     /locus_tag="PLM83_10240"
     CDS             complement(708..2000)
                     /locus_tag="PLM83_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOK71820.1"
                     /translation="MDVKELYRSKLISTQEAVGMVCSGQSIVSAMCAAEPPGLLGAIS
                     SRKCELENVTIMSCLLMRDYDFLKPEMKGHFLNEAWYYGQYSYMAHPGGTVTFLPNHL
                     SECGLKKAQIDPPDIFWGTATPPDKNGYMSLSLSLTYEKPMIAAAKTVVLEINENLPR
                     TLGDTHIHISQVDYVVENAVPLVEIDHITPSEVEMEIGRNVASLIEDGSTIQLGIGGI
                     PNAVTASLMDKRDLGVHTEMLTDGMVDLFNAGVVTGRAKTLWPEKMVGCFALGTRKLY
                     DFIDDNLGVELHGGHITNDPRVIAKNHKMVSINTTMQVDLAGQANSETLGTRQYSGGG
                     GALNTCMGAQMSPGGKSILALRSTAKGGAISTIVPCFGDGQSVTIPRADIDYVVTEYG
                     IARLKAMNIRKRVEALIAIAHPNYRDWLWSEAEALQYV"
     gene            complement(2041..>2390)
                     /locus_tag="PLM83_10245"
     CDS             complement(2041..>2390)
                     /locus_tag="PLM83_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK71821.1"
                     /translation="GSGRIINTSSVVGIYGNFGQSNYVATKAGVIGMTKVWAKELGRK
                     GITVNAVAPGFTMTEMMSTVPEKVLTGIRDRTPLGRLGEPRDIAYAYLYLASNEASFV
                     NGATIQVDGGLTL"
BASE COUNT          490 a          696 c          648 g          556 t
ORIGIN      
        1 aaccgcacgt tcgtccggcc cgcctggaca gagtcgcagg tagcaggttc gtacgttccg
       61 ccagagtaga taccccatgc ctcgtggtag gatccatcag ctatgaatga tgacgtgatg
      121 aacccgggct tgtcatcgcc ggtaagcact gccgcccccg ccccgtcccc gtaaaagaag
      181 gaagtgacgt cattggggtc ggccaacttg ctcatcatgt atgcgccgat aactagcacg
      241 taacggatcc acgggttggt cgcgatgtgg ccagctgcag ccgcaatacc tgtaggaaag
      301 gaagcgcagg cgcacccgat gtcgtaggtg ccggcatttt tcgccccgat cttgtgctgt
      361 gttaccacgg acgtggcagg ggttatgaaa tctgggctgt cagtgcccaa gatgatcaaa
      421 tccaggtctt caggggcaac ccccgcgtcc tccaaggcgg ccattgccgc ttttgctgca
      481 aggtcgcttg tggcccaatc gcgcggagca tagtaccgtt tcattatgcg cgaagactct
      541 tcaaacttga acaccacgtc acctaggcca ggtgcggcct gattgatcct ctcagccatg
      601 tgggcattgg atatttcggt ctccggaaga tagagaccgg tcccgatgat cctcgcatac
      661 cgttccatca acaacgcctc cgttaattcg gtagtctccg ccacgggcta cacatattgc
      721 aaagcctcgg cctccgacca caaccaatcc ctatagttgg gatgggctat ggcgatcaga
      781 gcttcgaccc ttttccgaat attcatagcc ttcagcctgg caatgccata ctccgtcacc
      841 acgtaatcga tgtcggctct gggaattgta actgactgtc cgtccccaaa acacggcact
      901 atggtggata tggcaccgcc cttagctgta gacctcagcg ccagtattga cttgcctccg
      961 ggcgacatct gcgcgcccat gcatgtattg agggcaccgc ctcccccgct gtactgcctc
     1021 gttcccaggg tttcggagtt ggcctggccg gcgaggtcca cctgcatggt ggtgttgata
     1081 ctcaccatct tgtggttttt ggctatcacc ctaggatcgt tggtgatgtg gcctccgtga
     1141 agctccactc ctaagttgtc gtcgatgaag tcgtagagct tgcgagtgcc caaggcaaaa
     1201 cagccgacca tcttctccgg ccacagcgtc ttcgcgcgcc ctgtcaccac cccggcattg
     1261 aagaggtcca ccatgccgtc ggtaagcatc tccgtgtgga ctccgaggtc gcgtttgtcc
     1321 atgagagatg ctgtcactgc atttggaatg ccacctatcc ccagctggat ggtgcttccg
     1381 tcctcgatca aggacgccac gttacgtcct atctccatct cgacctctga aggggtgatg
     1441 tggtctatct cgacgagggg gacggcattc tcgacgacat agtccacctg cgagatgtgg
     1501 atgtgcgtgt cgcctagggt gcgaggaaga ttctcgttta tctcaaggac gacggttttc
     1561 gccgccgcta tcatgggctt ttcataggtg agactgaggg acaaggacat gtaaccattc
     1621 ttgtcaggcg gggtggcagt tccccagaag atgtcgggcg gatcgatctg ggccttcttg
     1681 agcccacact cgctgaggtg gttcgggagg aacgtgactg ttccgcctgg atgggccata
     1741 taggagtatt gaccataata ccaggcctcg tttaggaaat gccctttcat ctcgggcttg
     1801 agaaaatcgt aatcccgcat caggaggcaa gacatgatgg tcacattctc cagctcgcac
     1861 ttgcgggaag agatcgcccc caaaagtccc gggggctctg ccgcacacat ggctgaaacg
     1921 atgctctggc ccgaacagac catgcccacc gcctcttggg tagagatcag cttgctgcgg
     1981 taaagctcct taacatccat taccgtccct cctcgcaaca tgtcttgtgc caaatcgtga
     2041 tcaaagagtc agaccgccat ccacctgaat cgtcgcgcca ttgacaaaac ttgcttcgtt
     2101 agatgcgaga taaaggtatg cataggcgat gtctcgaggc tcgcccagtc tgcccaaggg
     2161 agttctgtct ctgataccgg tgaggacttt ttcgggaacg gtggacatca tctctgtcat
     2221 ggtaaaaccg ggggctacag cgtttacagt gatccccttc cgtccgagct ccttggccca
     2281 gacttttgtc attccgatga ccccggcttt ggtggcaacg tagttggact gcccgaagtt
     2341 gccatagatg cccaccactg aagaggtgtt gatgatcctg cccgaaccct
//
LOCUS       DAOSAI010000371         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_29208_length_2380_cov_3.929032, whole genome shotgun sequence.
ACCESSION   DAOSAI010000371 DAOSAI010000000
VERSION     DAOSAI010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29208_length_2380_cov_3.929032"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLM83_10250"
     CDS             complement(<1..828)
                     /locus_tag="PLM83_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOK71822.1"
                     /translation="MKADLTIEERLAAVPEYTRFVTVDELYARAKAVAEARPDIAKLA
                     IVGKSTAGSEIPMVSIGDGPVSILIFVCPHPNEPIGAMMTYFLMEELLKDEDLRAGRT
                     WHIMPCVDPDGTRMNEGWFAGPFNIRNYAHDFYRPRPQDQVEWTFPIEYKTLKWDTPK
                     PETQALMKAIETTRPDVMYSLHNAGFGGAYYYISSGLSKEDYDELHRIPIDRGLPLSL
                     GEPEVPWAVELYPGVYESCPITASYDYYEQFAPGDPAQYITAGAGSDEWAKTVCDPFS
                     "
     gene            complement(914..1810)
                     /locus_tag="PLM83_10255"
     CDS             complement(914..1810)
                     /locus_tag="PLM83_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71823.1"
                     /translation="MSKERERIEETEGTIRSEFAHVVQVFFRHKLGVLGLVMLVAFVA
                     IAIFAPVVAPCDPYEMDLANPFLPPGSPGHILGTDNFGRDMLSRLIYGSRISLLIGIV
                     VVSISSVLGSLLGMVAGYYGGWVDALVMRLVEVFYSFPFLILVIAVMAILGPSIFNVM
                     WVLGVVSWPLYARLVRAQVMALKSEDFIKAASALGCSDARIMFRHILPNVLTPVIVSA
                     TLGIPSAILSSASLGFLGFGVQPPTPEWGAMVSEAKDFMLINSNMIIWPGLCIFLVVL
                     SFNFVGDALRDALDPRLAKQLN"
     gene            complement(1803..>2380)
                     /locus_tag="PLM83_10260"
     CDS             complement(1803..>2380)
                     /locus_tag="PLM83_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71824.1"
                     /translation="KRTWIDYTATTLAMLGVSLPGFYLGLLLILLFGVRLGWLPITGM
                     GAAGSGAWEYIKHLILPSITLGSGMAAILTRLTRSSVLEALSQDFVRTARAKGVREKT
                     VLYKHALRNALLPVITTFGLQFGSLLGGAVITETIFSWPGIGMLAVNAVKQRDIPVIQ
                     GTTLVFALSFVLITLIVDIVYVFIDPRIRYE"
BASE COUNT          589 a          673 c          586 g          532 t
ORIGIN      
        1 cgaaaacgga tcacatactg tcttggccca ctcatcgctg ccagctccag ccgtgatata
       61 ctgtgccggg tcgccaggcg cgaattgctc gtagtaatca taggaagccg ttatcgggca
      121 cgattcgtac actcccggat agagttcaac cgcccaaggc acttcaggct cacccaatga
      181 gagcggaagg ccgcgatcga tcggaattct gtggagttcg tcgtagtctt ccttgctcag
      241 cccgctcgat atatagtaat aagctcctcc gaagccggcg ttgtgcagcg agtacatcac
      301 gtccggacgt gtcgtctcta ttgccttcat caaggcctgc gtctcaggtt tgggcgtatc
      361 ccatttgaga gtcttgtatt ctattgggaa agtccactcc acctgatcct gaggccgcgg
      421 cctatagaaa tcgtgcgcgt agtttctgat gttgaatggg cctgcaaacc atccttcgtt
      481 catacgcgtc ccgtcaggat cgacacacgg catgatatgc cacgtgcgac ccgcacgcag
      541 gtcctcatcc ttcaggagct cttccattag gaagtaagtc atcatcgccc cgatgggttc
      601 gttcgggtgc ggacagacaa agatcaatat gctcacaggc ccgtcgccta tggagaccat
      661 aggtatctcg ctgccggctg tagattttcc gacaatggcc agttttgcaa tgtcaggacg
      721 ggcctccgcc accgccttgg cgcgtgcata aagctcgtca actgtcacaa atcgcgtgta
      781 ttcagggact gcagccaatc gttcttctat cgtcaagtct gctttcattt gcaaacctcc
      841 caagctacat atagtgctag tgctttcgca tgccgtaacc ttcgcggcat tggtgctatc
      901 cactcgactg cgttcagttc agctgtttgg caaggcgcgg atcgagagcg tcccgcaatg
      961 catcgcccac gaagttgaag ctgagaacaa ccagaaaaat gcagaggccc ggccagataa
     1021 tcatgttgga gttgatcaac ataaaatcct tggcctcgct caccatcgca ccccactcag
     1081 gggttggcgg ctgaactcca aaccccagaa aacccagaga tgcagaggaa agtatcgcgc
     1141 tgggtattcc aagcgttgcc gagactatca cgggcgtcag cacgttgggg agtatgtgcc
     1201 tgaacatgat cctggcgtcg ctacatccta acgcgctcgc agctttaatg aaatcttcgg
     1261 atttaagggc cattacctgg gccctcacca aacgtgcgta cagcggccag cttacaacgc
     1321 ccaacaccca catgacattg aatatgctgg ggcccaaaat agccatgacc gctatcacga
     1381 gaatgagaaa cggaaatgaa tagaacacct caaccagacg cattaccaat gcgtcaaccc
     1441 atccgccgta gtaacctgca accatgccaa gcaacgaccc gagaacactc gatatcgaca
     1501 caactacaat gccgataaga agagatattc ttgagccgta tatgagccgt gacagcatgt
     1561 ctcgcccaaa gttgtcggtg ccgaggatgt gtcctggcga gcccggcggc aggaatggat
     1621 tggcaaggtc catctcatat gggtcacatg gcgcaacgac cggcgcaaag atggctatgg
     1681 ccacgaatgc cacgagcatg acaagtccca aaacgccgag cttatgtctg aagaacactt
     1741 gcacgacatg ggcaaactca gaccttatcg ttccctctgt ttcctcaatt ctttctcttt
     1801 ctttactcat atctaatcct cggatctatg aatacgtata ctatatcgac gatgagtgta
     1861 atcagcacaa agctgagagc gaaaacaagg gttgtcccct ggattaccgg aatgtctcgc
     1921 tgtttgactg cgttcacagc caacatgcct atgcccggcc acgaaaagat cgtctctgtt
     1981 atcacagctc ctccgagcaa tgatccgaat tgcagcccga aggtggtaat tacgggcaac
     2041 agcgcattgc ggagagcgtg cttgtaaagg accgtcttct cacggacccc tttggccctg
     2101 gcagtacgga cgaagtcttg tgatagcgct tcaagcacgg acgatcttgt gagacgcgtc
     2161 agaatcgcgg ccattccact gcccaacgtt atagatggca gtatcaagtg tttgatatac
     2221 tcccaagcgc ccgatcccgc agcgcccatt cctgtgatgg gaagccatcc gagcctcact
     2281 ccaaaaagaa gaatcagcag aagtccaagg taaaaccctg gaagcgagac tcctagcatt
     2341 gcaagggtcg tcgctgtgta atctatccaa gtgcgcttct
//
LOCUS       DAOSAI010000372         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_29338_length_2371_cov_3.148964, whole genome shotgun sequence.
ACCESSION   DAOSAI010000372 DAOSAI010000000
VERSION     DAOSAI010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29338_length_2371_cov_3.148964"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1531
                     /locus_tag="PLM83_10265"
     CDS             185..1531
                     /locus_tag="PLM83_10265"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOK71825.1"
                     /translation="MTLHTFPDGFLWGAASSSCQIEGATLEDGKGESIWDRFCRIPGK
                     IVNGDTPAIACDHYHRFSEDVSLMESLKLNSYRFSIAWPRIFPVGGGRPNVKGVDFYN
                     RLVDELLAAGIRPMATLYHWDLPQALQDKGGWIARDTAKYFVDYAAYMFEALGDRVDL
                     WITHNEPWVAATLGYGTGEHAPGVSHMPSALAAAHNILLSHGEAVRAFRDLSRQGARI
                     GITLNMSPVYPASSNECDVLAARRWDGCLNRWYADPLFLGSYPEDIMELYLRAFGAPS
                     ISIEDMGIISTPTDFLGVNYYSRAVVKADPEAQPLELSHVRTNRPVTAMGWEVYPEGL
                     YDLLLRIEQDYDPPAVFITENGAAYDDQVSSGGLVDDRERMEYIRAHLIQLHRAMQDG
                     VEVEGYYLWSILDNFEWAFGYSKRFGIIYVDYTTLKRIPKSSARWYRSVIERGSVL"
     gene            1620..2204
                     /locus_tag="PLM83_10270"
     CDS             1620..2204
                     /locus_tag="PLM83_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOK71826.1"
                     /translation="MSLYEVPVGVSNRHLHLSRADLDTLFGEGYELTVRKDLSQRGQY
                     ASEETVDVVGPKGTISRVRVLGPVRKRTQVEISRTDSFKLGVQPPVRDSGDLDGSPGI
                     KLIGPKAEITIPVGVIVAKRHIHMTPEEAERIGLKDKDIVMTKVNGARALIFDEVLVR
                     VDDSYTWEFHIDTDEANAACLGNGDLVTIIKKVE"
     gene            2241..>2371
                     /locus_tag="PLM83_10275"
     CDS             2241..>2371
                     /locus_tag="PLM83_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017039584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOK71827.1"
                     /translation="MDCFFAAVEALDNPDYRGKPVIVGGPRDSVRAVVSTCSYEARA"
BASE COUNT          554 a          599 c          644 g          574 t
ORIGIN      
        1 ttcttcgtcg ctaacccgtt ccctctcccc gtcctactct aaagttacac catcatgacc
       61 gtgcccggag cttctgccca aatctgcggt tgaagtaata tgcatctcca acctcgctgg
      121 ggtcggaggg attcgtcgcc ctgtgttgaa gtttggaatt atcgctgaaa gtaggtgatg
      181 caacttgacg ctacacacat tccctgatgg ttttctctgg ggagcagcat cgtcttcatg
      241 ccagattgaa ggtgctacgc tagaagacgg caaaggcgaa tccatatggg acaggttttg
      301 tcgcatcccg gggaagatcg tgaacggaga cactccggcc atcgcatgcg atcattatca
      361 tagattctca gaggatgtct ccttgatgga atcattgaag ctcaattcat atcgcttctc
      421 cattgcatgg ccaaggatat tccccgtcgg aggcggacgc ccgaacgtaa aaggcgtcga
      481 tttctacaat aggcttgtag acgagttgct tgcagccggc ataaggccga tggcgactct
      541 gtaccattgg gaccttcccc aggctttaca ggataaggga gggtggatag cccgcgacac
      601 agcaaaatac tttgttgatt acgcagccta catgtttgaa gccttgggag atagagttga
      661 tctctggata acacacaacg aaccatgggt ggctgcaacc ttgggctacg ggacaggaga
      721 gcacgctcct ggtgtctccc acatgccgtc agcccttgcg gcagctcaca atatccttct
      781 atcgcatgga gaagctgtga gggctttcag ggacctttcc cggcaaggtg cccgcatagg
      841 gataaccctg aacatgagtc cggtctatcc cgcatcttcc aatgagtgcg acgtattggc
      901 cgcacgacgg tgggacggct gtctgaaccg gtggtatgcg gatccgctct ttctaggctc
      961 atatccagaa gatatcatgg agctttattt gcgggccttc ggtgcaccat ctataagtat
     1021 tgaagacatg ggaatcatct cgactccaac cgatttcttg ggagtcaact attacagcag
     1081 ggccgtggta aaagccgacc cagaggcaca gcctctcgaa ctctctcatg taaggacgaa
     1141 caggcctgta accgcaatgg gttgggaggt ttatcctgaa ggactgtacg acctcttgct
     1201 ccggatcgag caagactacg atcctcccgc cgtctttatc acggaaaacg gggctgccta
     1261 cgacgatcag gtaagctctg gaggcttagt cgacgaccga gaacgcatgg agtatatccg
     1321 cgcccacctt atccaattgc atagggcgat gcaagacggt gtggaggtgg aaggatacta
     1381 tctctggtca atacttgaca acttcgaatg ggctttcggc tattccaaga ggtttgggat
     1441 aatctacgtt gactatacta ctctcaagag gatcccaaaa tccagtgcca ggtggtatag
     1501 gagcgttatc gaaagaggta gcgtgttgta acgcccttgc tggccggcct caattgcttt
     1561 tgctggctac acaggctatc atgtaatcgt gtatcgcatt gacataggag gcaggggtaa
     1621 tgtcattata cgaggttcct gttggagtat ccaatcgtca tctccatctt tcccgtgcgg
     1681 atttggatac gttgtttggc gaaggctacg agctgacagt caggaaggat ctgagccaga
     1741 gagggcaata tgcatctgag gagactgtcg atgttgttgg ccccaaaggc actatttcaa
     1801 gagttcgcgt tctcggtcca gttcgcaaac ggactcaggt cgagatttcc cgcaccgact
     1861 ctttcaagct tggcgtgcaa cctccggtgc gcgactcagg tgacctggac ggttcgcctg
     1921 gaatcaagct cattggcccg aaagccgaga tcactattcc agtcggcgtc atagtggcca
     1981 agaggcacat ccacatgact cctgaggagg ccgagcgcat cgggttaaag gataaagaca
     2041 ttgtaatgac caaggtcaat ggggctcgag cactcatttt tgatgaagtt cttgtacgtg
     2101 tcgatgattc ctacacgtgg gagtttcata tcgacaccga tgaagccaac gcggcctgcc
     2161 taggcaacgg cgacctggta accataataa aaaaagttga gtgatttcgt gtgtggacac
     2221 gtaccatcgc ccatgtcgac atggactgtt tcttcgcggc agtagaggca cttgacaacc
     2281 ccgattaccg cgggaaaccc gttatcgttg gcgggcctcg tgacagcgtt cgggctgtgg
     2341 tgtctacgtg ttcctatgaa gcgcgcgcct a
//
LOCUS       DAOSAI010000373         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_29371_length_2369_cov_3.863008, whole genome shotgun sequence.
ACCESSION   DAOSAI010000373 DAOSAI010000000
VERSION     DAOSAI010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29371_length_2369_cov_3.863008"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PLM83_10280"
     CDS             complement(<1..467)
                     /locus_tag="PLM83_10280"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HOK71828.1"
                     /translation="MSILGTLVVGYVAWRIYKEHTEQNSHSSAPHKSPSSDPQGQKGG
                     TVTANMSEGTATASFSCGADVRIEKVVGVTYDNRQSVIAGMRAGEQVFLRREPSNRYD
                     ANAIRVERADGRQIGYLSRSVAAQLAGGLDRAGGEVEGVVYRLTGGTSDFPTRG"
     gene            1254..1703
                     /locus_tag="PLM83_10285"
     CDS             1254..1703
                     /locus_tag="PLM83_10285"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOK71829.1"
                     /translation="MKKLYRSRTDSMIGGVCGGLAEYLEVDPTIVRLAFVLLGLWSGM
                     GIAAYLILWVIIPYPDTGKASTSEVIREGAAEIRDSARKVAESTGQAFQQSQKPAANV
                     VIGIILVVLGIVFLARNLNVSWLRWLNAGTLMPIILIILGGLLLVRG"
     gene            1729..>2369
                     /locus_tag="PLM83_10290"
     CDS             1729..>2369
                     /locus_tag="PLM83_10290"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOK71830.1"
                     /translation="MSEFDNDGQRKRRTGIVLPSILILIGILLLLNNLGLVDWNVWGA
                     IVRFWPLILIAVGLELILGKRSISGAVGFMIILVIVAGSALAFRSPTSRGAITRTRVE
                     KPLSGATSAEIEIDMGVGALYLGSLANSSYLVSGTVETAAGGWLTQDFSITGTKARLV
                     LSENRGFPFFSMGKQPAMKWDLALNEGIPMRLDIRTGVGETRIDLEHVLATDV"
BASE COUNT          580 a          618 c          597 g          574 t
ORIGIN      
        1 ccacgagtgg ggaaatcgga ggttcctcct gtgagtcggt agacgacgcc ctcaacctct
       61 ccccctgctc tatcaagacc gccagccaat tgcgcggcaa cgcttcgtga caggtagcca
      121 atttgccgcc catccgctcg ctctaccctg atcgcgttag catcatacct gttcgaaggc
      181 tctcttctaa ggaacacttg ctctccggcg cgcattcccg ctatcacgct ctgcctgttg
      241 tcataggtta cgcctactac cttctctatg cgaacatctg ctccgcagct aaaggacgcc
      301 gtggctgtac cttcggacat attcgcagtg actgtacccc ccttctgccc ttgtgggtct
      361 gagctgggag atttatgcgg ggctgaagag tgggaatttt gctctgtatg ctccttgtag
      421 atccgccagg caacataacc aacgacgaga gtaccaagga tactcatgta gtcacacctc
      481 ctaaagatcc gctagccgac agatgatcct attgttacat taaaaggttc actctgactt
      541 ccaatttccc ttcgacatct gacgaaaaac tgcaatatgt ggctgcataa gccagccctt
      601 tgtcttgcat ccataaaaga agcggtccct agcctctctt agtctcgtta gaactggata
      661 cgttatagat agcgatagca agcggccggg atactccgcc gtagacatca caggcttgag
      721 cacggatcac agatgggttt agggaagagc cccatccatc agaaaactgg ggaaccgcta
      781 ggacatggca gcagagctaa aggaagaccc ctcatgactg cataagcgca aacacccttc
      841 ggatatgtta atctgataca tagccattgg ctctccctcc gtagataatt ggttctttca
      901 tgccctaatt ctattagaga accagtgacc ttttcatacc catcccaatc caatgcaaca
      961 ggactaaggc atttttacag cattatatcg gacgtaactt caggagaaac atctccttga
     1021 cctttctgca agcagagagt aatactgtta atactgaacc tgcttcatga gcgtctatta
     1081 tgagcgatta cgagatatgc tacggaagcg accaacataa taatggttag tggaatcata
     1141 tccgagcccc ctgccaggct ttgatgccag acgtgcttac ccaagcagca agaacatgtt
     1201 catgaaatgc ggaaaaagaa gagcctgctt tgggcacaag tgaggtgaac gcagtgaaga
     1261 agctttaccg aagtcgaacc gattcgatga taggcggggt atgtggcggc cttgcagaat
     1321 atctggaagt ggatccgacc attgtgcgcc ttgccttcgt gttgctaggt ttgtggtcgg
     1381 gcatgggcat agccgcctac ttgattcttt gggtgatcat cccatatccg gacacgggca
     1441 aggcctcgac ttctgaagtt atccgtgaag gtgcagcgga gatacgggat agcgcccgga
     1501 aagtcgcaga gtccacgggc caagcctttc aacaatccca aaagcccgct gccaacgtag
     1561 tcataggaat cattctagtc gtattaggga tagtctttct cgcacgcaat ctgaacgttt
     1621 cttggcttcg ctggctgaat gcaggcacgt tgatgccaat aatcctcatc atcctcggtg
     1681 gcctcctgct cgtaagggga taatatccat aagggaggag aatgagccat gtctgagttt
     1741 gacaatgacg gacagcggaa gaggcggaca ggcatagtgc ttccgtctat cctaatactc
     1801 attggtatcc tgcttctcct caacaacctc ggcttggtgg attggaatgt ttggggagca
     1861 attgtacgct tctggcctct catcctcatc gcagtcgggc tcgaactgat actgggcaaa
     1921 agatccatat caggggctgt aggcttcatg atcatactcg taatcgtcgc aggatccgcc
     1981 ttagcctttc gttctccgac gtcaagaggc gccattaccc gcacacgggt agagaagcct
     2041 ctgtctggcg caacgagcgc agaaatagag atcgacatgg gcgttggcgc tttgtatctg
     2101 ggctcgcttg cgaattcatc atatcttgtc agcggaacag tggagaccgc ggcaggagga
     2161 tggctcactc aggattttag catcaccgga accaaggcgc gcctcgtgct cagcgaaaac
     2221 agaggtttcc ctttcttcag tatgggcaaa caacctgcga tgaaatggga tcttgcactt
     2281 aatgagggca taccgatgag gttggatata cgcaccgggg tcggggaaac ccgcatcgat
     2341 ctcgagcatg tgctggctac tgacgttga
//
LOCUS       DAOSAI010000374         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_29433_length_2366_cov_3.335353, whole genome shotgun sequence.
ACCESSION   DAOSAI010000374 DAOSAI010000000
VERSION     DAOSAI010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29433_length_2366_cov_3.335353"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..196
                     /locus_tag="PLM83_10295"
     CDS             11..196
                     /locus_tag="PLM83_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family transcriptional regulator"
                     /protein_id="HOK71831.1"
                     /translation="MHAVGEGPLARCPKCGSPVRRLVPSRMGIAFHGSGFYATDHRSE
                     EYKREARKDGEPGTSAS"
     gene            complement(358..1455)
                     /locus_tag="PLM83_10300"
     CDS             complement(358..1455)
                     /locus_tag="PLM83_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71832.1"
                     /translation="MPEGEYRSPRHCCAGDQEVGAIAACYGVSVSALLELNPWLSPGE
                     MLAEGDVLVLPAVHGLDGSIAAISDRYGGPGLLIFNAEDWSSKMLASVGCSWASVAAL
                     SPDGRLVAIQGADGKIHIVDVHADLDRAITQPLAGLVTPRWSPSGESIMYEIDAHVYV
                     VDADSGAMKQSFEGQFASWLGDGYRMAYVHSGNVIMREMADGSAVLLTDAPERVCGLA
                     AASYGESVAYIACEDEGVVMCIVDVLTGETVRSKVDREVWPWSIAWAPDGKMIAYCVG
                     QRNLDNGQAVKVIILDPRDGSCRHEAEVVSDPRYSGLAWSPDSRAVAVSARIEGTGRY
                     GVYVVHPGGGLFQLTGTGNVVFPDWGRTAGQ"
     gene            complement(1571..>2366)
                     /locus_tag="PLM83_10305"
     CDS             complement(1571..>2366)
                     /locus_tag="PLM83_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71833.1"
                     /translation="LRLTDATSGEAILEGQNIFDLNEAEMKKLRRKMQIIFQDPYESM
                     NPRMDVQTVIAEPLILQGLASSAHDARDVVASALADVEMVPPEEYMGRYPHELSGGQR
                     QRVAVARALSVDPDFIVADEPVSMLDVSIRGEVLNLMLRLSRSRGVTFIYITHDLATA
                     RHICDRIAVMYLGKIVEMGTADEVIQTPLHPYTTALIGAVFVPDPRHRGFAEVLKGEI
                     PSPVNPPQGCRLHPRCPYTMDICKQVEPPLVLHEGTHQVACHRAER"
BASE COUNT          545 a          764 c          559 g          498 t
ORIGIN      
        1 attcgaattt ctgcatgcag tcggtgaagg accccttgcg cggtgtccca aatgcggctc
       61 cccggtccgt aggcttgtcc cctctagaat gggcatagct ttccacggat ccggctttta
      121 cgcgaccgat catcggtcgg aggagtacaa aagagaagcc cgcaaggatg gagagccagg
      181 aacctccgcc tcttaaaacg actaagctaa ttcaggcatc gatcggtagc cacactgatc
      241 gaggcgaccg ctacgtaatg cggtcgcctt ttaactatcc agtagccact acacccgttt
      301 cctcggcact ctcacggcac gtctgtgttt ctccaacttc cagcctcacc acagatgcta
      361 ttgtcctgct gtccttcccc agtcagggaa gactacattc ccagtccctg tcagctggaa
      421 aagtccacct ccggggtgaa ccacgtacac gccgtaccgc cccgtgccct ctattcgagc
      481 cgacaccgcc accgccctgg aatcagggct ccatgccaaa ccagagtatc ttggatcgct
      541 cacaacctcc gcttcgtgcc tgcagctgcc gtctcgcgga tcgagaatga tcacctttac
      601 ggcctgaccg ttgtctaaat tccgctggcc gacgcaatag gcgatcatct tcccatctgg
      661 agcccaggca atcgaccaag gccatacttc ccggtcaact ttgcttctca ctgtttctcc
      721 ggtcaggacg tccactatgc acataacaac accttcgtct tcgcacgcaa tgtaggccac
      781 actttccccg taagaagcag ccgcaagccc gcaaacccgc tccggcgcat cggttaacaa
      841 cacagcgctt ccatccgcca tttctcgcat gattacatta ccggagtgta cataggccat
      901 tctgtagccg tccccaagcc aagaagcgaa ctgcccttcg aatgattgct tcattgctcc
      961 cgaatcggca tcaacgacgt acacgtgcgc atcaatctca tacattatgc tctcgccact
     1021 cggcgaccac ctcggggtga ccagtcccgc taggggctga gtaatagccc gatccaagtc
     1081 ggcgtgcacg tcaacaatat ggatcttacc gtccgcgcct tggatcgcca caaggcggcc
     1141 atcaggcgaa agcgcggcaa ccgaggccca agagcacccg accgaagcaa gcatctttga
     1201 tgaccagtct tcggcgttga agatcaacag cccagggccg ccataccggt ccgaaatggc
     1261 tgcaatagat ccatcaagtc catggacagc aggaagcact agcacgtcgc cttctgcaag
     1321 catctctcca gggctcagcc aagggttgag ctccagcaac gcagagaccg aaaccccgta
     1381 gcaggccgca attgcaccta cctcctggtc ccccgcgcaa cagtgtctgg ggctacgata
     1441 ctctccttca ggcataaaaa acacctcgct tgcgaccgtc cctcgaacca aatatatgcc
     1501 tattgcacca agaaggtgct tagacagacc tggttcgaac agtcccaagc gaggcgcctt
     1561 atatccgtaa ctaacgctct gccctatggc acgctacctg atgagtgcct tcatggagca
     1621 ccaacggagg ctcaacctgt ttacatatgt ccatcgtata cggacatcta ggatgcaacc
     1681 gacatccttg tggcgggttc acggggctcg gtatctcgcc cttcagaact tctgcaaacc
     1741 cgcgatgcct cgggtcgggc acgaacaccg ctccgatcag agccgttgtg taaggatgca
     1801 aaggagtctg gatgacttca tcagccgtcc ccatttccac gatcttgccg aggtacatca
     1861 cggctatcct atcgcatata tgccgcgcag tggcaaggtc atgggtaatg tagataaacg
     1921 tgactcctct actccgcgaa agcctcagca tcaagttgag cacttcaccc ctgatcgata
     1981 cgtccagcat cgagaccggc tcgtctgcga ctataaaatc aggatccacg ctcaacgctc
     2041 gtgccacagc aaccctctgg cgctgtccgc cggaaagctc atgtggatat cttcccatgt
     2101 attcctcggg cggaaccatc tccacatcag caagggcaga tgccacaacg tcgcgtgcat
     2161 cgtgagccga tgaggcaagc ccctgcagaa tcaggggttc ggctataacc gtctgaacgt
     2221 ccatacgcgg gttcatcgat tcataagggt cctggaatat gatctgcatc ttccgacgca
     2281 gcttcttcat ctccgcctcg ttaagatcga agatgttctg gccttccaga atcgcctcac
     2341 cggacgttgc atcagtaagc ctcagg
//
LOCUS       DAOSAI010000375         2362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_29492_length_2362_cov_3.574339, whole genome shotgun sequence.
ACCESSION   DAOSAI010000375 DAOSAI010000000
VERSION     DAOSAI010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29492_length_2362_cov_3.574339"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..417)
                     /locus_tag="PLM83_10310"
     CDS             complement(46..417)
                     /locus_tag="PLM83_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOK71834.1"
                     /translation="MVELAQRYLGLCFGTLTMGPLQANISPDVGAALICEAVERGINF
                     FDTAELYGVYEYLRRVLHRVPREAIVISSRCYQYTYEGMRESLERALRDIGVDYIDIF
                     GLHEQESRLTLNGHVDAIRYL"
     gene            complement(436..>2362)
                     /locus_tag="PLM83_10315"
     CDS             complement(436..>2362)
                     /locus_tag="PLM83_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71835.1"
                     /translation="LGYPELEAMGKQSPYRGRPGLIGESLRLAGFAAAAVGNSDVAGV
                     QVRPGVLLVMDARGRVACGAVGAEIVEADPSAPFGISCDALAMAHATADALFRPNIAA
                     VTVDFGDMNRLGRYLSNLSSEARVEQLGKCYGKLDEIFRALFEAVGPPSDDLAYILFS
                     PGEPSSLGPVAIAGWQYPRGMLSSDTTRREGLVANLDLAATILDGLGVQQAWPHDGSP
                     MIGIRAGEEQNVAERLMRMAESLQIAASSRVPVLSVFIGTVVIAVFSVLAVALLGLRV
                     PAVFVFFAKFLLLSVAAQPLLILVAPILRIQGIAGTLLFASLGSAIISLLLIRTTSSC
                     MWASVGIAAASAVVIIADGLAGGTLAATSLLGHSAIVGARYYGIGNELMGVLAGSAAI
                     CLAALGEMWTGAERVVSRSGKATAGAVIGVGAISMLLGHPTIGANFGGMVTAAAIAVF
                     MGVMAFARSTLSRRAKVVAMVAIVVVAVGACAGMAWADAKGKRSSHIGRAWRLLTDQT
                     GADGADADVGAVQEREDAHGATEAIDMGLGQLGVIAWRKLEMNMRLLRYTAWTRVLLA
                     FLIALTVLAGKPFGLFRRFRDDYPIVYVSVMGGLAGALVAMAVNDSGVVACATVAMMP
                     AFALLGYVVDEILAEGA"
BASE COUNT          566 a          756 c          593 g          447 t
ORIGIN      
        1 cctagagacc gatactgccc tgatcaggcc atttcccttc gcgtcttaaa gataccggat
       61 cgcgtcaaca tgcccattga gcgtcagccg gctttcctgt tcgtgaagac caaatatgtc
      121 gatatagtct actccgatat cccgcaatgc ccgctccagg ctttcgcgca tgccttcgta
      181 cgtatactga tagcatctcg atgagattac tatagcctcc cgcggcactc ggtgcaagac
      241 tctgcgcaaa tattcataga caccatagag ctcggcagtg tcgaagaaat tgatgccacg
      301 ctccacggcc tcgcaaatga gggctgcgcc tacgtctggg ctgatattag cctgcaatgg
      361 ccccattgtc aatgttccaa agcatagccc tagatacctc tgtgccagtt cgaccaagga
      421 ttcctatttc cattttcagg ccccttcggc taggatctcg tctacaacgt agcccagcag
      481 cgcaaacgca ggcatcatag ccacagtagc gcacgccaca accccggaat cattcacagc
      541 catggccaca agcgcgccag ccaatccgcc catgactgat acgtacacga ttgggtaatc
      601 atcgcggaat cgtcgaaaca agccaaaagg cttgcctgcg agcaccgtaa gggcgatgag
      661 gaaggccaac agtacccgtg tccaggctgt atatcgtagc aggcgcatat tcatttccag
      721 ctttcgccat gcaattacgc ccagctggcc taggcccatg tcaatcgctt ctgtcgcacc
      781 gtgggcatcc tcgcgttcct gcaccgctcc aacatctgca tcagctccat ctgcgcctgt
      841 ttgatcggtc agaagccgcc acgctctgcc aatgtgggac gaacgtttcc ctttggcatc
      901 agcccaggcc atgcctgcac aagccccgac cgccacaacg actatggcga ccatcgctac
      961 gacctttgcc cggcgtgaaa gagtgcttct tgcaaacgcc atcacgccca tgaaaaccgc
     1021 tattgcagcg gcggtgacca ttcctccgaa gttggcgcct atcgtcggat gcccgagcag
     1081 catggagatg gcccctacgc cgataactgc gcccgccgtt gccttacccg acctggagac
     1141 aacgcgttcc gctccggtcc acatttcgcc cagtgcggca aggcaaatag cagctgagcc
     1201 cgccagaaca cccatgagtt cgttgcctat cccatagtag cgcgccccca ctattgcgga
     1261 atggcccaga agggacgttg cagcaagggt gcctccggca agaccatctg cgatgatcac
     1321 tacggccgat gccgcagcga tcccaacaga tgcccacatg catgatgaag ttgttctaat
     1381 gagcaacaaa gagatgatgg ccgatcctaa agatgcaaac aggagagtac cagcgatccc
     1441 ctgaatcctc aggatcgggg caaccagaat gagcaatggc tgggccgcca ctgacagtaa
     1501 caaaaacttc gcaaagaaca caaaaacagc aggtacccga agtccgagga gagcaactgc
     1561 gaggacactg aaaaccgcaa ttacaaccgt tccgataaac acgctaagta ccggaacccg
     1621 cgacgaagcc gcaatctgca gcgattctgc catgcgcatc agtctctctg caacattttg
     1681 ttcctctcca gccctgatgc cgatcattgg cgatccatca tggggccatg cctgctgaac
     1741 acccagaccg tcgaggattg tggcggccag atccagattg gcaacaagtc cctcgcgacg
     1801 agttgtgtcg cttgacagca taccccgggg atactgccaa cctgcgatcg ccacaggccc
     1861 gagggacgac ggttctcccg gagagaacag tatgtatgcg aggtcatcag agggcggtcc
     1921 cacagcctca aagagggctc tgaaaatctc atcgagtttt ccgtagcatt taccaagctg
     1981 ctcgacgcgg gcctccgagg agaggtttga taggtagcgc ccgagccgat tcatgtcacc
     2041 gaaatccaca gtcacggcag cgatattcgg ccgaaaaagg gcatccgctg tcgcatgcgc
     2101 catggccaaa gcgtcgcagg aaattccgaa tggagcgcta gggtcggctt cgacgatttc
     2161 tgcgccgacc gcgccgcaag caacgcgtcc ccttgcatcc atgaccagaa gaactccagg
     2221 ccgcacctga accccggcta cgtcggaatt cccgaccgct gccgccgcaa accccgccaa
     2281 acggagggat tcgccaatca gcccgggcct accccgataa ggcgactgct tacccatggc
     2341 ctccagctcc gggtatccca gg
//
LOCUS       DAOSAI010000376         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_29708_length_2351_cov_3.029181, whole genome shotgun sequence.
ACCESSION   DAOSAI010000376 DAOSAI010000000
VERSION     DAOSAI010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29708_length_2351_cov_3.029181"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLM83_10320"
     CDS             <1..225
                     /locus_tag="PLM83_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71836.1"
                     /translation="VLHCAYYVNPDQVIIGGKWLEAGDEFLDVLRQTVEEGFALDFSM
                     RPKISYSSLYPHTEVLAAVGLIINEFFQYK"
     gene            259..1419
                     /locus_tag="PLM83_10325"
     CDS             259..1419
                     /locus_tag="PLM83_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOK71837.1"
                     /translation="MGGIGAAKSMAKPISIILLVSVLATVVVSASAAGCTGAGGERMY
                     ESSGWRGFHMRFGSHDDVPNLKRVIIEYLIPMGVNVLILEVNTNFQFKSHPELSEGAH
                     TLTHEDAHELAALCRANGIRLIPLFECLGHQGWGGAPNSILRGYPEWDETPHVPVEAE
                     WPTIYCRSWCPLHPEVNPVIFDLMDELIEAFEADAFHVGMDEVFELADDGCPRCAGKK
                     KSDLFAKAVNDYHDHLVTEKGVEMLMWGDRLVDAVGLGYSRWDADVLGIYPAIDMIPK
                     DIIICDWHYDKKAAYPSVYWFFRKGFRVWPATWNDPDAPMAFMEFAYDAREKLDMKDG
                     MLGMLVTGWNATGENLLTALSGGDLGKDEGNIWGIATTLRTVMDEYKRKSAD"
     gene            1447..>2351
                     /locus_tag="PLM83_10330"
     CDS             1447..>2351
                     /locus_tag="PLM83_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71838.1"
                     /translation="MIPAKLRKTLMAVVALALSMYSATCICVAGGEGSRLVGAESAIP
                     YEEYLSVYSGAEEPQDEIVIDGAAYVSANMPGLETLRDHGGSDGESVLTAGRGYIEWE
                     FTVENPGFYNIEVEYCAAEGRSSALQRELWINGELPFSEAAFLTFSRAWRDTEAVRID
                     NQGNHLRPCQAEAPIWQKAVLSDQMGNYQRPLLFYFGKGNNRIRLVSILEPMIIRSVR
                     LFQMATPKSYAEVSADYGEKGFKAVAGFFEKVQGEDAYLKSSPTLFQVHDAGDPTVEP
                     YHHVLIRLNAIGGDRWTQPGEWIAW"
BASE COUNT          557 a          515 c          721 g          558 t
ORIGIN      
        1 gttcttcatt gcgcatacta tgtcaaccct gatcaagtca taattggtgg gaagtggctt
       61 gaagccgggg acgaattcct cgacgttctc cggcagacag tggaggaagg gtttgcactg
      121 gatttttcta tgaggccgaa gatctcctac tccagcctat acccacacac agaggttctt
      181 gcggcggtgg ggctcattat caatgaattc tttcaataca agtgagtgtt gagagcgctt
      241 gataggaggg cattgagcat ggggggcatt ggggccgcca agagcatggc aaagcctatt
      301 tcgataatcc tgctagtgag tgtgctggcg acggtcgttg tctctgcgtc agcagctggt
      361 tgcactggag ccgggggtga aagaatgtac gaatctagtg gatggcgtgg atttcacatg
      421 aggttcggtt ctcatgatga tgtccccaat ctcaagagag tcatcataga atacctcatt
      481 cctatgggag tgaacgtgct gatcctagag gtaaacacga atttccagtt caaatcccat
      541 cctgagcttt cagagggggc gcacaccctc acacacgagg acgctcatga actggcggcg
      601 ctctgccgcg cgaatgggat aagactgata ccgctctttg aatgccttgg gcaccaggga
      661 tgggggggcg cgccgaacag tatactcagg gggtatcctg aatgggatga gacgcctcac
      721 gtgccggtgg aggccgagtg gccgacgatc tattgccgca gctggtgtcc gctgcatcca
      781 gaggtcaatc cggtcatctt cgatctcatg gacgagctga tcgaggcctt tgaggccgat
      841 gctttccatg tcggaatgga tgaggttttt gagttggcag acgatggatg tccaaggtgt
      901 gccggaaaga agaagtcaga tctgtttgcc aaggctgtca acgattacca tgaccacctt
      961 gtcacagaga agggcgtaga aatgctcatg tggggcgatc gccttgtgga cgcggtgggg
     1021 ttgggataca gcaggtggga cgctgatgtt ctgggcatat atcctgcaat cgacatgatt
     1081 cccaaggaca tcatcatatg cgattggcat tatgacaaaa aagctgcgta cccttcggta
     1141 tactggttct tccgcaaggg attcagagtg tggcctgcga catggaacga cccggatgcg
     1201 cccatggctt tcatggagtt tgcgtatgat gcacgggaaa aactggatat gaaagacggg
     1261 atgcttggca tgcttgtgac agggtggaat gctacgggcg agaatcttct gactgcattg
     1321 agcggcggcg acttgggcaa ggatgaggga aacatctggg ggattgcgac cacactcagg
     1381 actgtaatgg acgagtacaa gcggaagtca gctgactaac acatataagc gctgggagtt
     1441 gggcttatga ttcctgccaa attgcgaaag acgttgatgg cagtggtggc gttagccttg
     1501 agcatgtatt cggcgacttg catttgcgtt gctggaggtg aaggctcaag gcttgtgggt
     1561 gcagagtctg ccattccata cgaggagtac ttgagtgttt acagcggcgc cgaagaacct
     1621 caggatgaga tcgtgattga tggggctgcc tatgtgtcag cgaatatgcc tggcttggag
     1681 acgctgagag atcacggtgg cagtgacggt gagtcggtcc ttactgccgg tcggggctac
     1741 atcgagtggg agtttaccgt ggaaaaccct ggcttctaca atatcgaagt agagtattgt
     1801 gctgcggagg gcagaagttc ggcgctccag cgagaactat ggatcaacgg tgagctgcca
     1861 ttctccgaag ctgcatttct cacttttagt agggcatggc gggacacaga agctgttcgc
     1921 atagataacc aggggaacca cctcagaccc tgtcaggctg aggctccaat atggcagaag
     1981 gctgttcttt ctgaccagat ggggaattat cagaggccgc ttttgttcta ctttggcaag
     2041 ggcaataaca ggataagact ggtatccatt ctggagccaa tgattatccg gtcggtgcgt
     2101 ctttttcaga tggctacccc caaaagctat gctgaagtca gcgcggatta tggagaaaaa
     2161 ggcttcaaag ccgtggcggg cttttttgag aaggtgcaag gagaggacgc atatctcaaa
     2221 tccagtccaa ctctgtttca agtgcatgat gcaggagatc cgactgtaga accataccac
     2281 catgttctta taaggctcaa tgcgattggc ggagacagat ggacccagcc tggagaatgg
     2341 attgcgtggc a
//
LOCUS       DAOSAI010000377         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_30203_length_2323_cov_3.268078, whole genome shotgun sequence.
ACCESSION   DAOSAI010000377 DAOSAI010000000
VERSION     DAOSAI010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30203_length_2323_cov_3.268078"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLM83_10335"
     CDS             complement(<1..200)
                     /locus_tag="PLM83_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOK71839.1"
                     /translation="MEGFCFVHAADLHLDSPFAAMKVDSPRLAQAFIEASRASLQALI
                     DVCIGHNADFLVLSGDVFDAERR"
     gene            complement(311..1393)
                     /locus_tag="PLM83_10340"
     CDS             complement(311..1393)
                     /locus_tag="PLM83_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71840.1"
                     /translation="MSVRDGRFAWKRAAIIISFLMSASILCQGGVEAINGSPSHGMRP
                     DQLYVLCLTPKAQFTSTMRVWNPSIGNLEVRAAGYGDVVVYSVKLLDCESCLAVSGNM
                     AACYNLPIDKSAMGRVGSVSGGGVVLVPLEESATGLLLAMLMPVGDNLPLLMRNYSIM
                     ESSGYFYRGRESVKLKLVPKVAGNVGRTVWIDKNSGVLLSLEDVNWRGEVIQRVQTLE
                     IDHAPELDAEGLRCARAALAMAANNRGTNKDATAEYASQVLGFKIAIPRWTPAGYELL
                     GVRVLEGVTGRYPLARGVGGVAHLVYYDGLGLISLYERCVPWWAASTLEPRAGNVIEW
                     EQNCIRYLLVGDIDPGLLLRMARSVL"
     gene            complement(1398..1808)
                     /locus_tag="PLM83_10345"
     CDS             complement(1398..1808)
                     /locus_tag="PLM83_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71841.1"
                     /translation="MDEVEAHLESCALCREKAQSLAALGEGLRCVPNPAWDIEAMVGL
                     VEAEIGSMGRRRAARVWRRSLLPLAAAAAALVILLTSVSSSAFRKQMVKHEEVLIRSH
                     YAVSSSHAGEALVSSRAWPMRGNIARLQMLEGWR"
BASE COUNT          551 a          753 c          523 g          496 t
ORIGIN      
        1 cgtcgctcgg catcgaacac atctcccgac aaaaccaaaa aatcggcgtt atgcccgata
       61 catacgtcga tcaaagcctg aagcgaggcc cttgacgcct ctataaatgc ctgggccaag
      121 cggggagaat ccaccttcat cgctgcaaat ggcgaatcga gatgaagatc tgccgcgtgg
      181 acaaagcaga atccttccaa aatagagcaa ccccctccga gcaggaattc acaccccaaa
      241 gggtattctc tgctcagagg ggtttaccct ccagcaatca tcgattgtct ggatcggccg
      301 ccggggccgt ttatagcacg ctccgggcca ttctgaggag gagtcccgga tctatgtcgc
      361 ctacaagcag atacctgatg cagttctgtt cccattcgat cacattccct gccctaggct
      421 ccaacgtaga agcggcccac cacggaacgc atctttcgta aagcgatatc agcccaagac
      481 cgtcgtagta cacaagatgg gcaacgcccc caacaccccg ggcaagcgga tatcgacctg
      541 tgacaccttc aagaaccctc acgcccaaaa gctcgtagcc tgccggcgtc catctgggaa
      601 ttgcaatctt gaagccgaga acctgcgagg cgtattctgc cgtagcatcc ttattcgtgc
      661 ccctattgtt cgcggccata gccagcgccg ccctggcgca tctaagcccc tccgcatcaa
      721 gctcaggggc gtgatctatc tccagggtct gaacgcgctg tatcacttct ccgcgccagt
      781 tcacatcctc aaggctgagc aagaccccgg agtttttatc gatccacaca gtcctcccga
      841 cattccccgc caccttcggc acgagcttga gcttcacgct ctcccgtccg cggtagaagt
      901 agcctgaaga ttccatgatg gagtaattgc gcatcaacaa cggcaggtta tcacccaccg
      961 gcataagcat tgccaaaagc agtcccgtgg cgctctcttc caagggcaca agcacgacgc
     1021 ctccgccaga cacagagccc accctgccca tagctgattt atcaatggga agattgtagc
     1081 atgccgccat gttgccgctc acagccaagc atgattcgca atctagcagc ttcacagagt
     1141 acacaaccac gtcgccatag ccggcggctc taacctccaa gtttccgatg gaagggttcc
     1201 atactctcat tgtggacgta aactgcgcct taggcgtcag acagaggacg taaagttgat
     1261 caggtctcat gccatgtgac ggcgacccgt tgattgcctc tactccccct tggcacagaa
     1321 tacttgccga catcaaaaaa cttatgataa tcgccgctct tttccaggca aatctgccat
     1381 cgcgcacact catcccatta ccgccacccc tcgagcatct ggagacgggc tatgtttccc
     1441 cgcatgggcc aggcacgact tgagacaaga gcctctcccg catggctcga ggagactgcg
     1501 tagtgggatc tgatgaggac ttcttcatgt ttgaccatct gttttctgaa cgcgctggac
     1561 gaaacggagg tcaaaagaat caccaacgcc gccgcagcag cagccagagg cagcaaagac
     1621 ctccgccaaa ctcgcgctgc cctcctcctg cccatgctcc cgatctctgc ctccactaga
     1681 ccaaccatgg cctctatatc ccaggcaggg ttgggtacgc acctcaaccc ttctccgagc
     1741 gcggccaaag actgggcttt ctcgcgacat aacgcgcacg attcaagatg agcttccacc
     1801 tcatccaacc tgaccccctg caactcgccg tcgatatgca tccccaacgt cagacaatcc
     1861 aagtgcttgt tgcaacatct ttcgtttcca tccgaacaca gacgtatcgg cttttcttgc
     1921 ttcatcagat gtcacgctac agagaccctc tccttcaggt gtgggaagga agtagcgttc
     1981 tccctccttt cgcacagcaa cgtgctcgcc gcttgcttca acctgatctg cttgcacagg
     2041 tcaaaacagc ctgtgctacg tctttcaaaa gcaaagcact cctcgccttt atgctcaacc
     2101 ttgtcaaata actaagaccc gtgaacatat ctggatgctt tgttggctct ctaagacacg
     2161 actcctcttt gcttttcacg gatcatccgg gccccactct gatgtactag gtaacataat
     2221 cagtcaaggc tagtcaacca ggcctgccga agcccccacc taagggctta gcatgcttgg
     2281 ccaagagagc gcttcatcag cttcaaacat ttctcgcagc ggc
//
LOCUS       DAOSAI010000378         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_30858_length_2289_cov_3.013876, whole genome shotgun sequence.
ACCESSION   DAOSAI010000378 DAOSAI010000000
VERSION     DAOSAI010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30858_length_2289_cov_3.013876"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLM83_10350"
     CDS             <1..226
                     /locus_tag="PLM83_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012369224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HOK71842.1"
                     /translation="NERITDESLVFITGGGRVWPLVRSHTILNNLGGRLDSLPVVLFF
                     PGSYDGLSLQLFNILDDDNYYRAFQAVPTR"
     gene            254..>2289
                     /gene="brxC"
                     /locus_tag="PLM83_10355"
     CDS             254..>2289
                     /gene="brxC"
                     /locus_tag="PLM83_10355"
                     /inference="COORDINATES: protein motif:HMM:NF033441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HOK71843.1"
                     /translation="MVSIKIRDMFVQPIDRDIKGVIKVGQTEEENLKQELEEYVVTNE
                     VRKHIGKFYDSFNKSIDRPYDKMGVWISGFFGSGKSHLLKILAALLENGEVDGQTAID
                     YIAPKLEDPMLEAEMRRAASVPKDVVLFNIDSKSDASSYIDRPTVVEVFARVFFEHLG
                     LCSNPTWLGYAEWKMIQRGFYEDFCQEFERVAGAPWTVRRETAMFEEDNIAQTMRNIG
                     ILGENSQSNFITSAKQFYEMSPEDFALMVREHLKSQGPDHKIVFLVDEMGQYIANDER
                     MILNLQTLAEDLGTHCHGRAWIAVTSQQAIDEVATEIKSKDFSKILGRFDTRLSLSSA
                     NVDEVIKKRLLEKTPVAHDTLTLFYHDKEAVCRNLFAFSDGTPGQRLYASPDEFAENY
                     PFVPYQFDLLQSVFEEIRKHGASGKHLAKGARSMLSAVQETTLAIADCEHGILIPFNR
                     FYDSMEEFLEPSVREIIERAKANPDLEPMDAEVLKTLFMLKYITNEMPPTRENVTVLM
                     LASINQDKLALRNSITESLDRLTRQNLVQKDGDLYIFLTDDEQEIDREISRVKLDESM
                     LCRHFYDTIFASGEIFKAKKIRYKNVYDFDFYQRIDSVRFGSAKAEIGMWIVTPFSYS
                     KDDPGELEEILRESALAPHECLMVLEHDKELVQQAESYLKLDTYLKNKSRAHLPT"
BASE COUNT          618 a          587 c          585 g          499 t
ORIGIN      
        1 gaatgagcgc attaccgatg aaagcctggt gttcatcaca gggggcggcc gggtttggcc
       61 actggtgcgc tcgcacacca ttttgaacaa cctcggcgga cggctggaca gcctgcctgt
      121 ggtacttttc tttcctggat catatgacgg actctctctg cagctcttca acatcctaga
      181 cgacgacaac tactatagag ctttccaggc agtcccaaca agatgaattg ctagccaatt
      241 gatgtgcacg ggggtggttt ccattaagat tcgcgatatg ttcgtgcagc cgattgatag
      301 ggacatcaag ggcgtcatca aagtcggcca gactgaagaa gaaaacctca agcaggagct
      361 tgaagagtat gtcgtcacaa atgaagtccg caaacacatc ggaaagttct atgattcctt
      421 caacaaaagc atagatcggc catacgacaa gatgggcgtc tggatatcag gattcttcgg
      481 ttcaggaaaa tcgcacttgc tgaagatcct cgccgcctta ctggaaaatg gcgaggtgga
      541 cgggcaaacg gccatagact acatcgctcc caagctggaa gatccaatgc tggaggcgga
      601 gatgcgacgg gcagcctcgg ttcccaaaga tgttgtcctc ttcaatatag attccaagag
      661 tgacgcctcg tcttatatcg acaggcctac agttgtcgag gttttcgcca gggtcttctt
      721 tgaacacctt ggcctctgtt cgaacccgac ctggctcggg tacgcggaat ggaagatgat
      781 acagcgaggc ttttacgaag acttctgcca ggagtttgag cgcgtagctg gtgcaccttg
      841 gacggtgcga cgcgagacgg caatgttcga ggaagacaat atcgctcaga cgatgaggaa
      901 catcggcatt ttgggtgaga acagccagtc caatttcatc acatctgcca agcaattcta
      961 tgagatgtct cctgaggact tcgccctcat ggtccgcgag cacctgaaaa gccagggtcc
     1021 ggatcacaaa atcgtcttcc tagtggatga aatgggacag tacatagcca acgacgagcg
     1081 catgatcctc aatcttcaga ctctggctga agacctgggg acccattgcc atgggcgtgc
     1141 ctggatcgca gtcacttcgc agcaggcgat agatgaagtt gcaacagaga ttaagagcaa
     1201 agacttctcc aagatcctgg gccgtttcga tactcgtctt tcgctatcgt cagccaatgt
     1261 ggatgaagtc atcaagaaac gcctcctgga aaagacgcct gtagcgcacg atactctgac
     1321 cctgttctat cacgataagg aggcagtctg caggaacctc ttcgctttct ccgacggaac
     1381 accggggcaa aggctctatg caagccccga tgaattcgca gagaactacc ccttcgtgcc
     1441 ataccagttc gacctccttc agtctgtatt cgaagagata cgcaaacacg gagcgtcagg
     1501 caagcatctg gcaaaaggag cgcgatccat gctatctgcg gtgcaagaga caactctcgc
     1561 catcgccgat tgcgagcacg gcattctcat tcctttcaac agattctatg attccatgga
     1621 agagtttctg gagccttctg tgcgggagat tatcgagcgc gccaaggcca atccggatct
     1681 ggaaccgatg gatgccgaag tgctcaaaac ccttttcatg ctcaagtaca tcaccaatga
     1741 gatgccgcct acaagggaaa acgttacggt tctcatgctc gcaagcatca accaagacaa
     1801 actcgcttta agaaacagca tcacagaatc gctcgatcgc ctaacgaggc aaaacctggt
     1861 ccaaaaagac ggtgacctat acatcttcct caccgatgac gaacaagaga tagataggga
     1921 aataagcagg gtcaaattgg acgaatcgat gctgtgcagg cacttctatg acacaatctt
     1981 cgccagcggc gagatattca aggccaagaa gatcaggtac aagaatgtgt atgacttcga
     2041 tttctaccaa cgcatcgata gtgtaagatt tgggtctgcg aaggccgaaa taggcatgtg
     2101 gatagtcacc cccttctcct attccaagga tgaccctgga gaactagagg agatcctgcg
     2161 ggagtcggcg cttgcgcccc acgagtgcct gatggttctg gaacatgaca aagaactggt
     2221 acaacaagca gaatcgtatt tgaagctcga tacatacctg aaaaacaaga gccgcgctca
     2281 tctgcccac
//
LOCUS       DAOSAI010000379         2280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_31001_length_2280_cov_4.312360, whole genome shotgun sequence.
ACCESSION   DAOSAI010000379 DAOSAI010000000
VERSION     DAOSAI010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31001_length_2280_cov_4.312360"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLM83_10360"
     CDS             <1..189
                     /locus_tag="PLM83_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HOK71844.1"
                     /translation="HAVGKRIPAVHGERRAGDPAVLVASSDKAMNELGWAPHYTDISD
                     IIASAWKWHSSHPHGYLR"
     gene            537..1790
                     /locus_tag="PLM83_10365"
     CDS             537..1790
                     /locus_tag="PLM83_10365"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK71845.1"
                     /translation="MTVVRAQHRRRDARVNYKLFFIDAFGYPLAFSLISTSTIMPLLL
                     TDLGASNLAIGLVPAIQSLGALMPGILVAPFLERLPLKKRLFVALGLAERLFILSMAG
                     IVAIWGVAKPSLAVKLFLLAWTLASLMAGINMTVYYAILPKCIPIDQRGGLFGWAGAL
                     SGVFGIIGAETAGYILETVEFPRNFALLFIMAFVILIASLVQFIWLREPPDEKSEGSR
                     SIKEYIAAASYEVREDRAYVWTIAALAVMAFAMSATSFYSTYAVRLFGAGTRDVGRLT
                     AFAVGATIVGMPALGWIADREGHKLSLEITAACFACGAALALAANNVTEMYPAVVFAN
                     IGMSGIAVSHNLILAEFAPTHTDVPMYTAFSWMLLAPFRTGAPILGGYISDIAGFPAM
                     FSISLAAGIAAVCIFLFAVEEPRRA"
     gene            complement(1877..>2280)
                     /locus_tag="PLM83_10370"
     CDS             complement(1877..>2280)
                     /locus_tag="PLM83_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71846.1"
                     /translation="LKEAVDRAVERATENFQEGAGILSAYMLKMKASAELLRTNPNTK
                     FVEEARPWLEEYVRWTDIGMHIAETVEKSGAYLVSSASSGRRGFSLHALPIVSARRKL
                     SRMMSDAVNFKTRVCGDVLLQLGRDLLRQLQ"
BASE COUNT          465 a          603 c          642 g          570 t
ORIGIN      
        1 cacgcagtgg gcaagcgcat tcctgcagtc catggcgaaa ggcgcgcggg cgacccggcc
       61 gttctggttg cctcttccga caaggccatg aatgagctcg gatgggcgcc acattatacg
      121 gacattagcg atatcatagc ctcggcctgg aagtggcata gcagccaccc gcatggatat
      181 ttgcgatgac gaaagaagac gggcaattcg gctgcgatgc ggacgcgact tgcccgtttt
      241 atccacgaag ggacgctttt gtcctggggt agacggttgg aggcaatgca aaactgaatg
      301 aggttgggat gcactcgctg ttagggcttg ggaagtgccc accttcacac gcctttatac
      361 attagtgtat aatacatctt gggcccacgg ccttcgcggt cgcgatgggc tttttttctt
      421 gataatccca ctgcaacgaa gcgggtgggg ccaaaaagag catgagcctt gcgttcttgt
      481 tatgttgctg ctgttcttgc aagaatagtt gtacatgatt taggagtgaa taatgcttga
      541 ctgttgttag ggcccaacat cgccgccggg atgcaagggt taactataag ctgtttttca
      601 tagatgcctt tgggtatccc ttggcgttct ctctaatatc gactagcacg ataatgccac
      661 ttctacttac cgatttgggt gcgtccaacc ttgccattgg gcttgtgcct gccattcaga
      721 gccttggcgc cctgatgcct ggcatactcg tcgctccatt tctggaacgg ttgcccctca
      781 aaaagaggct cttcgttgcg cttggcctgg cggagcggtt gtttattctc tccatggcag
      841 gaatagtggc gatatggggc gttgcgaagc cttctcttgc cgtgaaactg ttcctgttgg
      901 cttggaccct tgccagcttg atggcgggga tcaacatgac ggtatactat gccattcttc
      961 caaaatgcat tcccattgat cagaggggag ggctttttgg atgggcgggc gcgttgtcgg
     1021 gagtattcgg cataatcggc gctgaaaccg caggctatat cctggagacg gttgagtttc
     1081 cgagaaattt cgcgctgctg ttcatcatgg ccttcgtgat tctcattgca tcgcttgtgc
     1141 agttcatatg gcttcgcgaa cctccggatg agaagtctga aggatctaga tcaatcaagg
     1201 agtatattgc cgctgcatct tacgaggtgc gggaggacag agcctacgtc tggacgatcg
     1261 cagccttggc cgtgatggcc tttgccatgt cggcgacatc gttctactcg acatacgcgg
     1321 tcagactttt cggagcaggc acgagagatg ttggaaggct aacagctttc gctgtcgggg
     1381 ccactatcgt aggcatgccc gcattgggct ggatagccga tagagaggga cataaactga
     1441 gcctggagat tacggcagcc tgtttcgcat gtggcgcagc gctcgccctt gccgccaaca
     1501 acgtgacgga gatgtacccg gcggtagtgt tcgccaacat cggaatgagc ggaatagcgg
     1561 tgagccacaa tctcatcctt gccgagtttg cgcccaccca cactgatgtg ccaatgtaca
     1621 ccgcgttttc ctggatgctg cttgccccgt tcaggactgg cgcaccaatc cttggaggat
     1681 acatctccga cattgcagga tttccggcga tgttctccat atcgctcgca gccgggatag
     1741 cagccgtctg tatctttctt tttgcggtag aagaaccgcg ccgcgcctga tgcccaaagc
     1801 ctagatcatg caagatgcct caccaggtct gctttcgggt gaaggcgcag cgcgcccggt
     1861 gcgcctttat ccgaggtcat tgcaattgcc tcaacaggtc gcgaccgagc tggagcagga
     1921 catccccgca caccctggtc ttgaaattca cggcatccga catcattctc gacagcttgc
     1981 gtctggcaga gactattggc aacgcgtgca gcgagaaacc gcgacgtccg gatgacgccg
     2041 atgatacaag ataggctccg gatttttcga cggtttctgc tatatgcata ccgatgtctg
     2101 tccaccgaac gtattcctcc agccacggac gggcttcctc aacgaacttc gtgtttgggt
     2161 ttgtgcgcag caactccgca gacgccttca ttttgagcat gtatgcggaa aggattccgg
     2221 ctccctcttg aaaattctca gtggcgcgtt ccacggcccg atctacggct tctttgagca
//
LOCUS       DAOSAI010000380         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_31023_length_2279_cov_3.410522, whole genome shotgun sequence.
ACCESSION   DAOSAI010000380 DAOSAI010000000
VERSION     DAOSAI010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31023_length_2279_cov_3.410522"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLM83_10375"
     CDS             <1..589
                     /locus_tag="PLM83_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOK71847.1"
                     /translation="PEFTLVFMLSIISTFFISRDHPRFSENLLKALPRSWRKQARKVR
                     GELLAGFVGYLRSRFVLMCITGIMTVIGFSIMGISYAWLLGLVCALLDLMPVVGPSLL
                     ILPWAGYHFVVGHFGFGLGLIIVLAVNSIVRQIAEGKVMGENLDLHPLVALVSIYVGI
                     RLFGTKGFIAGPLAVIFIKAVYQSVILPMFPFDEE"
     gene            593..1747
                     /gene="dxr"
                     /locus_tag="PLM83_10380"
     CDS             593..1747
                     /gene="dxr"
                     /locus_tag="PLM83_10380"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOK71848.1"
                     /translation="MTSICILGSTGSVGVQSLEVARSLGLKVSGLSAYSEVELLAEQA
                     RQFGPAMVGIGDVRAEARARALFSRMSTPPELFFGPEGVSQVAAAAEHDLVVNAIVGF
                     AGVWPTLAAIDAGHDVALANKETLVAAGSVVMDRARQEGVAIRPIDSEHSAIYQCIQG
                     QGREAVARIILTASGGPFIDRYDFADISVAEAISHPNWNMGPKISVDSATMINKALEV
                     VEARWLFDVDSEMIDIVVHRESVIHSMVEYCDGSIIAQLGTADMRTPIQYALTCPERV
                     EGLAAKLDLSSIGKLTFEKPDLSRFPGAGLGHEALRHKGVVPAVMSAANEEAVRLFLH
                     GAVRFVDIVDLVMEAMESSPRIEEPSLDDIVEADVWAREFVRGRCRSCTR"
     gene            1735..>2279
                     /locus_tag="PLM83_10385"
     CDS             1735..>2279
                     /locus_tag="PLM83_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOK71849.1"
                     /translation="MYALAFLVVLGIVVLVHEYGHFAAARACGVKVYEFAIGFGPQLF
                     SWKRGETLYAVRALPLGGMCRLAGMDDSDLPEEQMEEDDPRSFLNRPIWQRILIVVAG
                     PLMNFVLAVVILSVLFSSYGVPMASVVEVFDGSPAEAAGFESGDKIVSIDGKPVASVA
                     GFINTVASSAGLSLVVGLAGTA"
BASE COUNT          453 a          528 c          704 g          594 t
ORIGIN      
        1 tcccgaattc acgctggtct tcatgctcag cattatctct acgtttttca tatcccgcga
       61 tcacccgcgt ttctccgaga atcttttgaa ggcgttgccg agatcgtggc gaaagcaagc
      121 gcgaaaggtc agaggcgaac tgcttgccgg gtttgtgggt tatctgagat caagatttgt
      181 attgatgtgc ataactggca taatgacggt tatcgggttt tctatcatgg gcatcagcta
      241 tgcgtggctt ttggggttgg tctgtgcact cctcgacctg atgccggtcg taggcccgtc
      301 tcttctcatt ctgccatggg ccgggtatca tttcgtagtt ggccacttcg gttttgggtt
      361 gggtctgatt atagtgttgg cggtcaactc tattgtgcgc cagattgctg agggaaaagt
      421 gatgggggag aatctcgacc ttcatccgct cgtcgctctc gtgtctatat acgtgggaat
      481 tcgcctgttt ggcacaaagg gctttatagc agggccgctg gcagtgatat ttatcaaagc
      541 agtatatcag tcggtaatcc tgccaatgtt tccatttgat gaggagtaag atatgacatc
      601 tatttgcatt cttggctcca ccggatcggt gggtgttcaa tctcttgagg ttgcgcgttc
      661 tctagggctg aaggtgagtg gcttatcagc ttattccgaa gtcgagctcc tagctgagca
      721 ggcgcgccag ttcggtcccg ccatggttgg aattggcgat gtgcgcgccg aagcgcgggc
      781 gagagcttta ttctcccgca tgagtacgcc gccggaattg ttttttgggc cagaaggcgt
      841 ttcacaggtc gcggcggcag ccgaacacga tttagtggtc aatgccatcg tggggtttgc
      901 gggggtatgg cctaccttgg ctgcgatcga tgctgggcat gacgtggcgc tcgccaacaa
      961 ggagacactt gtggctgcag gttctgtggt aatggatcgg gctcggcagg agggagtagc
     1021 cattaggccg atagacagcg agcattcagc catataccaa tgcatccagg gacagggcag
     1081 ggaagctgtc gcacgcatca ttcttacagc gtcaggcggc ccattcattg acaggtatga
     1141 ttttgctgat atctctgtgg cggaagccat atcccatcct aactggaata tgggtcccaa
     1201 gatttccgtg gattcggcca cgatgatcaa caaggcgttg gaggtcgtag aagctcgctg
     1261 gcttttcgat gtggattcgg agatgataga tatcgtggtc caccgcgaaa gcgtcatcca
     1321 ttcgatggtg gagtactgcg atggctcgat aatcgcgcag ctggggacgg cagacatgcg
     1381 cactccgata caatatgccc tgacatgccc cgaaagggtc gaagggctgg ctgccaagct
     1441 cgacctgagc tccatcggga aactgacatt tgagaaacct gatctttcca ggtttcctgg
     1501 ggcgggattg ggacatgaag ctttgaggca taaaggcgtg gttcctgcag tcatgtcggc
     1561 cgcaaatgag gaggctgtgc gcctatttct tcacggtgct gtgcgtttcg tcgacattgt
     1621 ggacctcgtg atggaagcga tggaatcatc tccccgaata gaagaacctt ctttggatga
     1681 catagttgaa gcggatgtat gggccaggga gtttgtgaga ggaaggtgtc gctcatgtac
     1741 gcgctagcct tcttggtggt gcttggaatt gtcgtgcttg tccacgagta tggtcatttc
     1801 gccgcggcga gagcttgtgg ggtgaaggtc tacgaatttg ccattggttt cggaccccag
     1861 ctgttttctt ggaagagagg ggagacgctc tatgccgtgc gagccctccc attagggggc
     1921 atgtgcaggc ttgcaggtat ggacgattcg gacctcccgg aggagcagat ggaggaggat
     1981 gatcccagga gcttcctcaa ccgccctata tggcagagaa tactcatagt tgtcgcgggg
     2041 ccacttatga acttcgtcct tgccgtggtc atactctcgg ttctcttctc gtcatacggt
     2101 gtccccatgg catcagttgt cgaagtcttc gatggaagtc cggcggaggc agcagggttt
     2161 gagtcgggcg acaagatagt aagcatagac ggaaagcccg tagcgagtgt ggctgggttt
     2221 ataaacaccg tggcttcgag cgcgggcttg tcgctcgtcg ttggattggc tggcacggc
//
LOCUS       DAOSAI010000381         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_31066_length_2277_cov_3.164266, whole genome shotgun sequence.
ACCESSION   DAOSAI010000381 DAOSAI010000000
VERSION     DAOSAI010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31066_length_2277_cov_3.164266"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLM83_10390"
     CDS             <1..454
                     /locus_tag="PLM83_10390"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HOK71850.1"
                     /translation="ARIKLISDGDVAGAISTALPSTGVDLLMGIGGAPEAVITAAAMK
                     CLGGEIQTKLWVRNEEDTARARENGFSDFDRVYSSEDLARGDSLIFAATGITDGDMLQ
                     GVRFYGPKATTESIVMRMLTGTVRRIHTTHDLSRKTLRSFQAGQEMTL"
     gene            complement(451..1413)
                     /locus_tag="PLM83_10395"
     CDS             complement(451..1413)
                     /locus_tag="PLM83_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71851.1"
                     /translation="MCERAAAKVERVSVVWRRSAIALIAAGALIFPMFACQLAGTQAS
                     PAHTGGRSPGIGPWKCQTGIRAFPAIIRTDLGRFLWFIDAKVGQVVLCNTGNTEALIR
                     IDVRGITQDGEGNFIFIPASEEVAAALSVSHSSFRLAPGVSRTIRITIDPRYVRRRDR
                     FGLCAALEVRSSPSGRDGAAPGLSALPGINIPVIVRLPGNHVNRLSLEDVDIGESRED
                     GMSAIRVKVRNRGETYILPGGTLSLEPEGGVRAKTGKLGLAVTPGLVLPGCARWLEAE
                     IPQGSLNVGHYEATLIMSVNGKTVLNERFMLKMNEDGCVSIRVP"
     gene            complement(1416..1964)
                     /locus_tag="PLM83_10400"
     CDS             complement(1416..1964)
                     /locus_tag="PLM83_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71852.1"
                     /translation="MKRRSKAASLVAIRCRVTTVLLLALMSIAWAGSMCSARETGTIQ
                     LAGKVPPVMELVVRNSVIQWDGLRPGDNECPTSAAFSVISNVPFKVTIMSDNPNMQAT
                     EAGPLHGTTLSHPLEWSSDSSCCYTPVSTSPQTVIYGEYHSELVLHTLEFRQKIDITD
                     PPTIESNEYYRGTVILSLEAAS"
     gene            complement(2097..>2277)
                     /locus_tag="PLM83_10405"
     CDS             complement(2097..>2277)
                     /locus_tag="PLM83_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOK71853.1"
                     /translation="VETDSPYLPPSPYRGKRNEPAYVVEVVRKIAEIRGVSTEEVARV
                     TAANTMRIFSISPEG"
BASE COUNT          538 a          670 c          567 g          502 t
ORIGIN      
        1 cgcccgcatc aagctgatat ctgacggaga tgtagcgggt gcaatatcca cagcccttcc
       61 aagcacagga gtggaccttc tcatggggat aggcggcgcg ccggaagccg tgatcacagc
      121 ggccgcaatg aaatgcctgg gcggcgagat ccaaacaaag ctctgggtgc gcaatgagga
      181 agacaccgcc cgggccagag aaaacggatt cagcgacttc gaccgcgtct actcctcgga
      241 agacctcgct cgaggcgaca gcctgatatt tgccgcaaca ggaatcaccg acggagacat
      301 gctccaggga gtgcgcttct acggtcctaa ggcaaccacc gaatccatcg taatgcgcat
      361 gcttacaggg acagtgcgaa gaatacatac tacccatgat ctttctcgga aaacactcag
      421 gtcattccaa gccggacagg aaatgactct ttaggggaca cgaatggata cacaaccatc
      481 ttcattcatc ttgagcatga acctctcatt gagaacagtc tttccgttca cactcattat
      541 caatgtggcc tcgtagtgtc cgacattcaa actgccttgg ggaatttcag cctcaagcca
      601 tctggcacat cccggcagca ccagtccggg agtgaccgca agccccagtt ttccggtttt
      661 ggcccggacg ccgccttcgg gttcaaggct gagggttccc ccgggcaata tgtaagtctc
      721 gcccctgtta cgcactttaa cacgtatcgc actcatgccg tcctcgcggc tttcgcctat
      781 gtccacatct tccagggaaa gcctgtttac atgattccca ggcaatctga caattaccgg
      841 gatgttgatc ccaggcaagg cagaaagacc cggcgcagcg ccgtccctgc cagacggcga
      901 agaccgcact tccaatgctg cgcacaagcc gaaacggtca cgccgcctga catacctcgg
      961 gtctatcgtg atccttattg tgcgggacac gcccggcgca agccgaaatg aactgtgcga
     1021 taccgacaaa gccgctgcaa cctcctcgga cgcagggatg aagatgaagt tcccttcccc
     1081 atcttgagta atcccgcgaa catcaatcct tatcaatgct tctgtattac ccgtattgca
     1141 aaggacaacc tgtcccactt tggcatctat gaaccacagg aatcgcccaa gatccgtacg
     1201 gataatcgca ggaaatgccc tgatccccgt ctggcatttc caaggcccaa taccgggcga
     1261 cctgccacct gtatgagcag gggaagcctg cgtgccagcc aactggcacg caaacatggg
     1321 gaagatgagg gcacccgctg cgatcagagc gattgcactt ctacgccaga ccacagatac
     1381 tctctcaacc ttggcagctg ctcgttcgca catttctacg aagctgcctc gagcgaaaga
     1441 atgacggttc cccggtagta ttcattcgac tcgatagtag gcggatcagt gatgtcgatc
     1501 ttctggcgga actctagggt atgcaaaaca agttcagaat ggtattctcc gtagatcaca
     1561 gtctgaggcg atgtgctcac cggggtgtaa cagcatgaag aatccgagct ccactccagg
     1621 ggatgactga gcgtggttcc atgcagtggg ccggcctctg ttgcctgcat gttggggtta
     1681 tcagacatta tagttacctt aaagggaaca ttgctgatta cggaaaaagc cgctgatgtc
     1741 gggcattcat tatctcccgg cctcagcccg tcccattgta taacagaatt cctgactacg
     1801 agctccatca caggcgggac cttgcccgcc agctgaattg tacccgtttc tctggccgag
     1861 cacattgaac ctgcccacgc aatcgacatg agagcaagca gcaataccgt tgtcactctg
     1921 caacggatcg cgacaagaga cgccgcttta gaacgccttt tcatacaaga atccctcctt
     1981 gcttctttcg atctgcagcg gttcacaatc ttgttgtgcc cgccgcggat cagttcaagg
     2041 aggggtattc ttttaatgcc atattatccc tgtcaccctt gcgggcgatt tccgcgtcat
     2101 ccttcgggtg atattgagaa tatcctcatg gtattggcag ccgtgacccg agctacctct
     2161 tccgtcgata cgcccctgat ttcggcaatc ttccgtacaa cttccactac atatgcaggt
     2221 tcgtttcgct tgccccggta cgggcttgga ggaagatacg gcgaatcagt ctccacc
//
LOCUS       DAOSAI010000382         2273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_31147_length_2273_cov_3.178539, whole genome shotgun sequence.
ACCESSION   DAOSAI010000382 DAOSAI010000000
VERSION     DAOSAI010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31147_length_2273_cov_3.178539"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLM83_10410"
     CDS             <1..678
                     /locus_tag="PLM83_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HOK71854.1"
                     /translation="KTFCNALAKVIVALTASSWIVLSGIGWRVPQQMKAVLLTASSLY
                     VGFFLFSLFLMNDPQLIETVIAPILRSRFVARLLKPDGSERVLKSIRKWISEFQVALA
                     HYWECERWTLLGVIYLSVAGWLALILVPTMILAGLGVRAPSEQVMANVILFHMVSSYV
                     PTPGASGAAELGFAELFRMVVPPDLIGVAVALWRLLTYYFTLLVGGTLTLSRLLRGSV
                     GPAGDEG"
     gene            1442..>2273
                     /locus_tag="PLM83_10415"
     CDS             1442..>2273
                     /locus_tag="PLM83_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOK71855.1"
                     /translation="MRLTMLRATCILALAVLLLAVSASGALAAPEAVQDLTGVEEHKP
                     YFIIHLDAISSQVFFEMYDAGLLPNISELFSEGKVIPYAISPFMPGTEMIYPRLKQGA
                     PASSDDPISWTSINRHTGEYKTYLDNVIGLWSWAPRYAFGQLLTGVLGLDALSRLSLA
                     NIERLLRDYGVVEFLWLASDTVGHIKGIDAQKSSAMRLDSMLAPLVRYTKDSPGSLNL
                     VLYCDHGMSMIDEYVLTERAMAEVAGDKALLCFYPNVYLKDERHAPEVARALGERHEV
                     D"
BASE COUNT          477 a          517 c          687 g          592 t
ORIGIN      
        1 aaaaccttct gcaacgctct tgcaaaggtt attgtggcat tgactgcgtc ttcatggatc
       61 gttctgtctg gcataggttg gcgtgtgccg cagcagatga aagcggtgtt gttgactgca
      121 tcctcgctgt acgtgggatt cttccttttc tcgctgttcc tgatgaatga cccacagctg
      181 atcgagactg ttattgctcc gattcttcgc agccgatttg ttgccaggct gttaaaacct
      241 gatggaagtg aacgggttct caaatcgata aggaagtgga tctccgagtt tcaggtcgca
      301 cttgcgcact attgggagtg tgaacgttgg accctgctgg gagtgatcta tctgagtgtg
      361 gccggatggc ttgcccttat tctggtgcct acgatgatcc tcgctgggct cggggttagg
      421 gcgcctagcg agcaagtgat ggccaatgta attctctttc atatggtttc atcgtatgtt
      481 cctactcccg gggcgagcgg cgcggccgag ctggggtttg ccgagctgtt caggatggtt
      541 gttccgccgg atctgatcgg tgtggctgta gccctttggc ggctgctcac ctactacttt
      601 accctgttgg tggggggcac tttaaccttg agtcggttgt tgcgaggcag cgttgggccg
      661 gcaggggatg agggatgagg ttggggccct aggcacagga gctgaggtgt tgggcgtaga
      721 ggatagttct tctgagtcat caaggaaatc ggggcgtgtt ggagcctggt tatacccaac
      781 atcctgctgc gcgatttttt ccggaggtct gcgcaagccg ggtagccgac atatctgcag
      841 ccatcgcttg gacaaatgtc aaggaggtat ggcttttcgg agggattcat ggagttcacc
      901 gttaaagccc agatagacta ggaaggtttt ggccatgtcg tcgcactcca tccaggtgcg
      961 tggattgaac ggacttgatc aggctgagtc ctcgcagcta tggaatgagt gtcgaggata
     1021 tggcggaact cggcaagccc tctggccaac atggtgcttt ctcagggtta gttcgatgag
     1081 ataggactga ttgacctcgc aaaacatgag gtcggcccca ccttacttga ttgaaacgcg
     1141 ttgccatcga ggaggcgtag atttgggtgt gggaattcag ggcagtgggt gaatatcccc
     1201 aaacgaggtc tcggcagctg aaaggagact gagacttaat ggagtatgaa caatgataag
     1261 cggatgagag agcatgagac ggcaatcgaa agagcctgtt aaagtctgaa gtagtggtgt
     1321 gaatttgaca gaaggatttc ggggtattga cggaacagcc atcatgcgat tctcttcgga
     1381 tgtaatacag actcaaaccg aatagggaca ggccatattg cactatttcg gaggtatttt
     1441 cttgagatta actatgttga gagcaacatg tattctggca ctagcagtgc ttttgttggc
     1501 agtgagtgct tcaggagcgt tagcggcgcc ggaggcagtg caagatttga ctggagttga
     1561 agagcacaag ccgtacttta tcatccacct tgacgccatc tcgtcacagg tgttctttga
     1621 gatgtatgac gccggcctcc ttcccaacat ttcggagctg ttttccgaag gcaaggtcat
     1681 cccgtatgct atttccccat ttatgcctgg taccgagatg atctaccccc ggttgaagca
     1741 gggggctccg gcgagttcgg atgatcccat ttcgtggact tcaatcaacc gccataccgg
     1801 tgaatacaag acctaccttg acaatgttat agggctttgg tcgtgggcgc cgcggtatgc
     1861 gtttggtcag cttctcacag gcgtgctagg gcttgacgcg ttgtcccgtc tgtcgctggc
     1921 gaacatcgaa cggcttttac gggattatgg tgtagttgaa ttcctgtggt tggcatctga
     1981 caccgtgggc cacataaagg gcatcgatgc gcagaaatcc agcgcaatgc gtctcgacag
     2041 catgctggct ccgttggtgc gttatacgaa agactcgccg ggcagcctaa atcttgtcct
     2101 ctactgtgac cacgggatga gtatgattga tgagtatgtc ctcacggagc gtgccatggc
     2161 cgaggttgca ggtgacaagg cgctgctctg cttctacccc aacgtgtatc tcaaagacga
     2221 gcggcatgcc cccgaagttg caagggctct tggagagcgg cacgaggttg att
//
LOCUS       DAOSAI010000383         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_31172_length_2272_cov_3.097429, whole genome shotgun sequence.
ACCESSION   DAOSAI010000383 DAOSAI010000000
VERSION     DAOSAI010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31172_length_2272_cov_3.097429"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1402)
                     /locus_tag="PLM83_10420"
     CDS             complement(140..1402)
                     /locus_tag="PLM83_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK71856.1"
                     /translation="MKRAIAFILTGVLFFALAVPMGALAQKKVTFTLWTKEGEVTGGV
                     LEDIVELSKEFAKSRPGLTIEVVSYGVEELRQNFQAAAFAGQGPDLLWTVSDHAGPFV
                     AMKIIKPVDNIFGKDFLKEFVRPGLECVELDGKTWGVPISVGNHLMLLYNKNLLKTPP
                     KNTDEMIKVGKELTRDLNKDGVPDQYGLVYNLNEPFFFAPWLGGFGGWPLDGTKPTLN
                     TPAMVNALQFVHDLKFVHKIVPVECDYDAADALFKEGKAAMLINGDWSLTSYQTPEVR
                     KKVDLGVARIPMVSQTGLWPTPMTSGIYFMLPDYLTGEKLQIAKEFVEFISSDKVQLR
                     FLEKHMRLPATASALKNPKIASDPILKGSADQMVVGKPMPTVPEMRAAWDAIRPNQEA
                     VMAGKMSPKNAAKAMQDACEKLIREMTK"
     gene            complement(1598..2167)
                     /locus_tag="PLM83_10425"
     CDS             complement(1598..2167)
                     /locus_tag="PLM83_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOK71857.1"
                     /translation="MSLGHRNIAFLDGYDTGLPVQPLLHGGPAVDGCGTDSATPMWGI
                     SEARRAGYAEALDAHDIIFDPDLILYGDYTQEGARRAVFQMLQERPDVTAIFAISDVM
                     AIGAMQAVRESGRKVARDVAVVGYDDIESASYVRPALSTVKQQGEDMGRSAVRQVLRL
                     IGNPAEAPAKVILPVELVVRESCGAGRGR"
BASE COUNT          533 a          676 c          545 g          518 t
ORIGIN      
        1 cacgaggatc cttcccgctt ctgtcagccc acgggacgca atttcccaag tcaaagggat
       61 cacgtcaatc gcctcctggg gaaattggtc cgggctgtgc cgaaaaaaca acccggaccg
      121 ttcgcttcat ggtttccgtc tatttcgtca tttctcgaat caacttctcg caagcatcct
      181 gcattgcctt ggcagcgttc ttaggcgaca tcttgcccgc catgaccgct tcttgattag
      241 gcctgatggc gtcccaagcc gccctcattt cagggacggt tggcattggc ttgccaacaa
      301 ccatctgatc cgcagagcct ttgagaatcg gatcagaggc tatcttcggg ttcttgagcg
      361 ccgacgcagt agctgggaga cgcatgtgtt tttcgaggaa acggagttga accttgtcag
      421 atgagatgaa ctccacaaac tcctttgcga tttggagttt ctcgcccgtc aggtaatcgg
      481 ggagcatgaa gtatattccc gatgtcatgg gcgtcggcca gagccctgtc tggcttacca
      541 ttgggatgcg ggccacacca agatcaacct ttttcctaac ctcaggcgtc tggtaactcg
      601 tgagtgacca gtcaccattg atcaacatgg cggccttgcc ttccttaaat agtgcatccg
      661 ctgcatcata gtcgcattcc acaggcacaa tcttgtggac gaatttcaga tcgtgcacga
      721 actgaagagc attgaccatc gccggagtgt tcagcgtggg ctttgtgcca tcgaggggcc
      781 agcctccgaa ccctccaagc catggagcaa agaaaaacgg ctcgttcaga ttgtaaacga
      841 gtccgtactg atcgggcaca ccgtccttgt tcaggtcgcg cgtcagttcc ttgccgacct
      901 tgatcatctc gtcggtattc ttaggaggcg ttttaagcag attcttgttg tacagaagca
      961 tcagatggtt cccaacgcta atgggaactc cccacgtctt gccgtcaagc tccacgcatt
     1021 caagccctgg ccttacaaac tccttcaaga aatccttccc aaagatgttg tcgacaggtt
     1081 tgataatttt catggcgaca aatgggccag catggtccga cacagtccaa agaagatccg
     1141 gcccctgccc ggcaaaggca gcagcctgga agttctgcct caattcctcg acgccataac
     1201 tgaccacttc aatggtgagc ccgggacggg atttagcaaa ctcctttgac agctcgacaa
     1261 tatcctccag cacccctcct gtcacttcac cttccttggt ccaaagagtg aacgtgacct
     1321 tcttctgagc caaagcgccc atcggcaccg ctagagcgaa gaacaggaca cccgtcaata
     1381 tgaatgcaat cgcccgcttc atcattggtt acctccttcc aagttcgacc gtcgcacggt
     1441 cggagacaac atcggaccct tcaaacctcg ccgtactcga gtcaacatta atcgctcaca
     1501 gacaacatag cccataccaa accggattcc tcaatctcga gacttctgaa gtcctttacc
     1561 acctcctgat cgacgagtag cagggccaac ggcaacttca ccggccccgc cccgcaccac
     1621 aagactctct aaccaccaac tcgactggaa gaataacctt tgcaggcgcc tcagcagggt
     1681 tacctatgag gcgcaaaacc tgtcggacag cactcctccc catatcctct ccctgttgct
     1741 tgacggtgga aagtgcaggc cgtacatagg aggcagactc tatgtcatcg taaccgacta
     1801 ctgccacgtc cctggcgacc ttccgccccg actcgcgcac agcttgcatg gcgccaatag
     1861 ccatcacatc gctaatagca aagattgccg tgacatccgg acgctcctga agcatctgaa
     1921 acacggcacg tctcgcccct tcctgagtat agtcgccgta aagtatcaag tccggatcga
     1981 agatgatgtc gtgggcatcc aaagcctcag cataccctgc gcggcgtgct tcggagatcc
     2041 cccacattgg cgtagcagaa tcagttccgc atccatctac ggcaggtcct ccatgcaata
     2101 gcggctgaac aggcaatccc gtgtcatatc cgtcgagaaa agctatgttg cgatggccta
     2161 aggaaatgag gtgttcggtc gagtcacgtc cgatataatg ctgtaaaaag gccttaaccc
     2221 tgttgtggtg ggtggggatg ggtatggaaa ggccactggt tctctagtag aa
//
LOCUS       DAOSAI010000384         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_31237_length_2268_cov_4.705829, whole genome shotgun sequence.
ACCESSION   DAOSAI010000384 DAOSAI010000000
VERSION     DAOSAI010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31237_length_2268_cov_4.705829"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLM83_10430"
     CDS             <1..853
                     /locus_tag="PLM83_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK71858.1"
                     /translation="AVVALVIYPFLFNLQLAFSNMSMYRFKEFSVGLSYGIQNFLDVF
                     RAPVLQNVTFAQLLLRTVVWTAVNVFFHVLGGLFLAILLNRPLRGKAIYRTLLTIPWA
                     IPQVIAALAWKNEFHYQYGFINVLLQKLGFAPVQWLSDPKWAFVAVIIVNIWLGIPFM
                     MVIILGGLQSISPDYYDAAQIDGASGFQQFRAITMPLLKPVLTPAIILGVVWTFNNFN
                     VIYLITKGGPMEGTDILVTSLYKAVFESYRYGFGAAYSTVILIILLIFTWIYLKVTKG
                     MGEVADA"
     gene            850..1725
                     /locus_tag="PLM83_10435"
     CDS             850..1725
                     /locus_tag="PLM83_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOK71859.1"
                     /translation="MKKESSRIHSFFRSGKGDSPAKKLLIHLILIMASVIAVYPFLRV
                     VAISLRPGSQLLSTDLSLIPSNATLENYRTLIFEKDFTIWLWNSLVVSLTTVIVGIVL
                     SASAAYAFSRWDFPGRKQGLVFLLTTQMLPPSMLLLPIYVMVVRLQLFNTYTGMILAH
                     SVGAVPFSIWLLKGYYDTIPRELEEAAFVDGASRLEAFYKIIIPLSTPALGVCGLFSF
                     MTSWSEFMMARVMLQKADLFTWPLGFQKLLDQFTTQWGVFSAASVLVAVPAMLLFMYS
                     SKWLISGLTLGGVKE"
BASE COUNT          504 a          526 c          595 g          643 t
ORIGIN      
        1 tgccgtggtt gcactggtca tatacccatt cctattcaat cttcagctcg cgttcagcaa
       61 catgagcatg tacagattca aggagttctc ggttggcctt tcgtatggca tccagaattt
      121 tcttgatgtg ttcagggcac cggtgcttca aaatgtgacc tttgcccaac tgctccttcg
      181 gacggtggtg tggacagcgg tgaacgtctt cttccatgtt ctaggggggc tctttctggc
      241 gatccttctg aacagaccgc tccgcggcaa ggcgatatac cgcactttgc tcacaatacc
      301 ttgggcgatt ccccaggtca ttgccgccct tgcctggaag aacgaattcc attatcaata
      361 cgggtttata aatgtcttgc tacagaaatt aggattcgct ccagttcagt ggttgtcgga
      421 tcccaaatgg gcttttgttg cagtcataat agtcaatatc tggttgggca tcccattcat
      481 gatggtgata atactgggcg gactccagag tatatcgccg gactactatg atgctgctca
      541 gatagatgga gcatcagggt ttcagcaatt cagggcgatc actatgccgc tgctcaaacc
      601 cgtgttgacg cctgcgatta ttctcggggt agtttggacc ttcaataatt tcaacgtcat
      661 ttatctcata accaagggcg ggcctatgga gggaactgat attctggtca cttcgcttta
      721 caaggcagtt ttcgagtctt atagatatgg ttttggggct gcctactcca ctgtgattct
      781 catcatcctc cttatcttca cgtggatata cctgaaggtg accaaaggga tgggggaggt
      841 tgcggacgca tgaaaaagga aagttcgagg atacacagct ttttcaggag cgggaaggga
      901 gacagtcccg caaagaaact cttgattcat ctaatcttga taatggcgtc agtgatcgcc
      961 gtatatccgt ttctgcgagt ggtggccatt tcgttgcgac cggggtcgca gttgctctcg
     1021 actgatcttt cgctgatacc gtccaatgcg acattggaga actaccgtac actcatattt
     1081 gagaaggatt ttacgatctg gctttggaac agccttgttg tctcgcttac cactgtgatt
     1141 gttggcatcg tgctatcggc cagtgccgcc tacgcttttt cgagatggga ttttcccggg
     1201 cgaaaacaag gccttgtctt tctcctgaca acgcagatgc tccccccgag catgttgttg
     1261 cttcctatct atgtgatggt tgtgaggctt cagctgttca acacctatac gggaatgata
     1321 cttgcgcatt ctgtgggcgc tgtgccgttt tcgatctggc tgctcaaggg atattacgat
     1381 actattccca gggaactcga ggaagcggcc ttcgttgacg gcgccagtcg cctcgaagcc
     1441 ttctacaaga taatcatccc tctatctact ccggcattgg gtgtttgcgg gctgttcagt
     1501 ttcatgacct cgtggagcga attcatgatg gccagagtca tgctgcagaa ggccgacctg
     1561 tttacgtggc cattgggttt ccagaagctt cttgatcagt tcactaccca gtggggagtc
     1621 ttctcagcgg cttcggtgtt ggtagccgtg ccggctatgt tgctgtttat gtattcctcg
     1681 aaatggctca tatcagggct cacgcttgga ggggtgaagg agtaacgata tctgggggtt
     1741 atcgtggata ctgatgcagt acagatggga agacgaaaaa ccttgatagc tccaatgtcc
     1801 attggggcag tatggcagcc aggtgtagct tcaaatgaag ctcagcctgg ctgccatggt
     1861 attaaaactg agcatgggtg cactttatga agtaccggcg gaagtctcat gcgctttatg
     1921 tcgtgggtca cagatggaga ctacatctga tcaaccttga ctgcatttaa gtttgccatg
     1981 acacccatac gcatgttcag tgccacatct ccgtgggaac cgtttcttta ggtttttatc
     2041 gaattaatgg tagacgcctt catctatata gtgacaatgg aaatagcgta taggacaaaa
     2101 ggaataacaa gggcagtagc gaatctactt ccgtactgca tgtccagaca cgtagtttcg
     2161 ggcagcaagc gtgatgttgc tcttttcgat gccttcgtca aacatgcgca gcaaggcagg
     2221 acgtgtaatc ctatgtggaa ggggcgtttg cggtattgca gatggacc
//
LOCUS       DAOSAI010000385         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_31238_length_2268_cov_4.056484, whole genome shotgun sequence.
ACCESSION   DAOSAI010000385 DAOSAI010000000
VERSION     DAOSAI010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31238_length_2268_cov_4.056484"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLM83_10440"
     CDS             <1..301
                     /locus_tag="PLM83_10440"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512794.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOK71860.1"
                     /translation="VVRDPKEAARGADILYTDVWASMGMEAEQEKRKKDFQGYCIDQE
                     IVDAANPDCIVLHCLPAHRGEEITHEVMEGSHSAIWDEAENRLHVQKAILALVMS"
     gene            1406..>2268
                     /locus_tag="PLM83_10445"
     CDS             1406..>2268
                     /locus_tag="PLM83_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71861.1"
                     /translation="MKKLAVILAAAMIATVALPVAAANVSVGGKLDSKMMYDFGADTP
                     SAETNLKLNLNVGPIGGQTITGVIEFNPWLIAEKNGCGMAPKPVENGDPVPGAIRRAY
                     LEAVGPYWTDGPAVTSRLGDLELAYSPFILSTEALNGMSVSGIAYGPVEAGVFHGFDK
                     GIRARGARVAAGVANVAVDASAVKVGDDELDYAAYAIATPIPGLALDATYAAQTNTKA
                     AAIRANAAAKVPYLPLDVIATAGYRRTDLTFNPTYRWMEEGNPVDGEQGKAAVTAGLA
                     TSYAGFDVALNG"
BASE COUNT          548 a          585 c          662 g          473 t
ORIGIN      
        1 ggttgtgcgc gatccgaagg aggcggctcg aggagccgat atcctatata cggatgtatg
       61 ggcgagcatg gggatggaag cggagcagga aaagagaaag aaggacttcc aaggatactg
      121 catcgatcag gaaattgtgg atgcagccaa tcctgattgt attgtgctgc actgccttcc
      181 tgcacaccgt ggcgaggaga tcacccatga ggttatggag gggtctcatt cggctatttg
      241 ggacgaagcc gaaaacagat tgcacgttca gaaagccata cttgcgttgg ttatgagctg
      301 atctggtgtt tgagaaggat aatcggggcg cggatcccta tcgccgtagc aggggtccgc
      361 gctttgtcgt aggggcgaga ccgtggcggc ccgggttgga tagggcgctc caaacgagat
      421 agggtgggac ggctgcagaa aaccgtgctg acgctgggta ggggtcaggt ccatctttcg
      481 ttgcaggcga aacgacccgt ggagcatttg agcaccccca aatggctgta gcccgacagg
      541 tgggatgcga gtgtggcgat agcgcagaac ttgaccaggg gttgcctggg ctgtttcaga
      601 tcgtgtgatt ggcttgcgcc gattctaagg ccaaagaacc ggtacaacgc tgtctttctg
      661 gtcaaccgga cgctcagccc ttttcattat ttgcagaacg cacaggccct gctgcatgtt
      721 ctcttcctca aggtatttcc gagatcatcg acacttgctt caaagattga cgatttccag
      781 ctacgtcgtt gctccgtcag cgtaacggca attgtgccat attccccagc gctgcaacag
      841 aagaccaggc ggtgccgagc aattcccgag tatcctctgc gtggccaagg agtctttgtt
      901 gcgtatggct cagaccctag ctgccgaaga ggggtcaagc actttttgag acttgttgtt
      961 accagttgac gcaaagaatt ttgccaatac gcagcaatat atgtcccaga tacctccgta
     1021 cccacaactt acacctgttc aaacccgcct ttacctgcag gaacaacgct cacaaactag
     1081 aattgatatt gcagaacagg cgatgatgcc gaggcgaata ccgcgacccc gcctgttcgc
     1141 gaccgcaata tgatgccttt gccatggtga ggggcgagaa aggaggtgca cccccgatag
     1201 ctcgaaagcc catgttccag ggcgccatac taagcagcaa cgctgtgtat gcagaggaag
     1261 gcaacatgga cacttccccg gctaacagcg agacgcagca cacatcggag caactatatg
     1321 tgccacaaca atgagacgca gctatagacg aacacatcat aacagcaaat gcggcactat
     1381 acgaagtaac caagggggaa caaacatgaa aaagcttgca gtgatacttg cagcagctat
     1441 gattgcaacc gtggcactgc ctgtcgcagc cgcgaacgtc agtgtcggcg ggaagctcga
     1501 cagcaagatg atgtatgact ttggcgccga tactcccagc gccgagacga atctcaaact
     1561 caacttgaac gttgggccca taggcggcca gactatcacc ggtgtgattg aattcaatcc
     1621 gtggctgata gctgagaaga acggctgtgg catggcgcct aagcccgtag agaacggtga
     1681 tccagtaccg ggagcgataa gaagggcgta tctcgaggca gtcggtcctt actggactga
     1741 tggtccagct gtaacaagca ggcttggaga tctggaactt gcatactctc cgttcatact
     1801 ctctacggag gctctgaacg ggatgtcagt ttctggcatt gcttacggac ctgtcgaggc
     1861 tggcgtgttc cacggattcg acaagggaat ccgcgccagg ggcgctcgcg tagcggctgg
     1921 agtagccaac gtggctgttg atgcttcagc agtgaaggtc ggagacgatg aactggatta
     1981 cgcagcctac gccattgcga cgccgattcc gggcctcgcg cttgacgcaa cctatgctgc
     2041 acagaccaat acgaaggcag cggcaatcag ggccaatgcg gccgctaagg ttccatatct
     2101 tccgttggat gtgatagcca cggctggtta tcgccgcaca gaccttacct tcaatcccac
     2161 ctacaggtgg atggaagagg gcaatccagt cgacggtgag caggggaaag ccgctgtcac
     2221 cgctggcctt gccacatcat acgccggatt cgacgtggct cttaacgg
//
LOCUS       DAOSAI010000386         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_31259_length_2267_cov_4.375678, whole genome shotgun sequence.
ACCESSION   DAOSAI010000386 DAOSAI010000000
VERSION     DAOSAI010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31259_length_2267_cov_4.375678"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLM83_10450"
     CDS             <1..375
                     /locus_tag="PLM83_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOK71862.1"
                     /translation="YGELSVKTQYFTEPVLLFEVPPTAFDPPPSVRSAVVQMTMRSAP
                     PVEADADMMFSIVSAGYGKRRKTLRNALLASHLFAGDRDAVEEVLSCASVDGGRRAET
                     LSLEEFAAISRAYVAYRAGENG"
     gene            372..845
                     /locus_tag="PLM83_10455"
     CDS             372..845
                     /locus_tag="PLM83_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HOK71863.1"
                     /translation="MKYFISPTKGRLTFEETFDDIMAFVHDMPDEEYKLIIGTDSQVN
                     GDTCFVSAIVIHRVGRGARYYYCREHETIGTGLRQRIFYEASKSLDLASQVAHKLAEN
                     GYADVNIEIHLDVGVNGKTKELNRDIVGMISGTGFSARIKPDSYGASKVADKHTR"
     gene            1140..2093
                     /gene="yabG"
                     /locus_tag="PLM83_10460"
     CDS             1140..2093
                     /gene="yabG"
                     /locus_tag="PLM83_10460"
                     /inference="COORDINATES: protein motif:HMM:NF017401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HOK71864.1"
                     /translation="MANRNRIREGDIVTRKSYGGDVFFQVIRVDSYAVGDATATLKGL
                     NMRLLADAPISDLSIVEERQLLDFKRKVMRESAEHLRTVRVRRITEEQARYKKERRRS
                     RGSAALAEDIFDLPGRVLHIDGDADYLRDCMKYYKELGVPVVGEHVPPQDQTAAVGPL
                     LDEHSPDVLVLTGHDALKKKNSDRASMSSYWNSEAYVNAVRVARQHEMDKDGLVIIAG
                     ACQSFYEALMEAGANFASSPGRVLIHCLDPVLLAEKVVNTPVEEIIRIEEAIENTITK
                     RPGVGGIQTRGKMRTSMPKTPMGVFGAGANRDNQSARNYMP"
BASE COUNT          563 a          539 c          644 g          521 t
ORIGIN      
        1 tatggcgagc tttccgtgaa gacgcagtat ttcactgaac ccgttttgct ttttgaagtg
       61 cctccaacag cgttcgatcc gcccccctcg gttcgctctg ctgtggtcca gatgactatg
      121 aggtcggcgc ctcctgttga ggcagatgcg gacatgatgt tctccatcgt gagcgcgggg
      181 tatggcaaac gccgtaaaac tctcaggaat gcactgcttg cgtcccattt gtttgcagga
      241 gacagagatg ctgttgaaga agtcctttca tgcgcgtctg tcgatggagg aaggcgtgca
      301 gagaccctct ccttagagga atttgcggcg atatcgcgtg catacgtcgc atacagggca
      361 ggtgaaaacg gttgaaatac ttcataagtc ctactaaagg acgccttaca ttcgaagaga
      421 cattcgatga catcatggcg tttgtccatg acatgccgga tgaagaatac aagctgatca
      481 ttggaacgga ttcccaggtg aacggtgaca cctgctttgt cagcgcgata gtgatccaca
      541 gggtcggacg tggggcccgc tattactact gtcgcgagca cgagactatt ggcacaggcc
      601 tcagacaacg gatcttttat gaggcgtcaa agagcctcga ccttgccagc caggttgccc
      661 acaagcttgc ggaaaacgga tatgccgatg tcaacatcga gatacatttg gatgttggcg
      721 ttaatggcaa gacgaaggag cttaaccgcg acatagtcgg gatgatcagc ggaaccgggt
      781 tttccgcccg tataaagcca gattcctatg gtgcctcgaa ggtggccgac aagcacaccc
      841 ggtaggctgc tttccgatcc gcgtccatgt ccccatgttt tggcacactc tctgaacagc
      901 caaggctgta tcagcgctct tccatgttct ccaaaacctt cgattatgcc tggggattgt
      961 atgcttgcaa gcgctaaact ctgtgcatct cggcacgttt ctccttaccc ttatcccaca
     1021 tatcctgaac gggaactgaa gatcgttgtt taaacagggt acgcttatgg cacgcttttg
     1081 cgatgggcga ggtccatagc gaagattcga tcagttcctg gaaaaggagg gaagggccct
     1141 tggctaacag aaacagaatt cgagaaggcg acatagtcac ccggaagtca tacgggggcg
     1201 acgtcttctt tcaggtaatt cgggttgact catatgccgt gggcgatgca accgcaaccc
     1261 tgaaagggtt gaatatgcgc ctccttgcag acgctccaat atcggatttg tctattgtag
     1321 aagaacgtca actactcgat ttcaagcgga aggtgatgcg tgaatcggca gaacatctca
     1381 gaacggtgcg tgtgcggagg ataaccgagg aacaggcgag atacaagaag gaacgcaggc
     1441 gatcgagagg gtcagctgct ttggccgagg atatctttga ccttccgggc agggtgcttc
     1501 atatagatgg ggatgccgat tacctcagag attgcatgaa atactataag gaactaggag
     1561 ttcctgtcgt gggagaacat gttccccctc aagatcagac tgcggccgtt ggtccattgc
     1621 tcgatgagca ttcacctgat gtgctggttc ttactggcca cgatgctctt aaaaaaaaga
     1681 attctgatcg cgcttcaatg agcagttact ggaattcgga agcatacgtg aacgcagttc
     1741 gtgtggccag acaacacgag atggacaaag atggccttgt gatcattgca ggggcatgcc
     1801 agtcgtttta tgaggccttg atggaggcgg gtgccaactt cgcaagttca cccgggaggg
     1861 tgttgatcca ctgcctcgat ccagtcctat tggcggagaa agtggtgaac acgcctgtgg
     1921 aggagatcat caggatagaa gaggccatag aaaatacaat aaccaagcgg cctggcgtgg
     1981 gtgggattca gactaggggg aaaatgcgca cgagcatgcc caagacgccc atgggggttt
     2041 ttggggcagg cgcgaacagg gacaatcaat cagctagaaa ctacatgcct tgacgtttgc
     2101 acggaatagc cagaggaatc taacgttgac tgatgtgagt caggcagcgc atgaggaagc
     2161 tccagagaaa atctatgtct ggagcttgct tgttcgcccg gcaagtctgt tgagtcgact
     2221 gactggaaga agtcccgcta cccaacgggt gtacatccat gcaaggc
//
LOCUS       DAOSAI010000387         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_31446_length_2257_cov_3.145777, whole genome shotgun sequence.
ACCESSION   DAOSAI010000387 DAOSAI010000000
VERSION     DAOSAI010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31446_length_2257_cov_3.145777"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..416
                     /locus_tag="PLM83_10465"
     CDS             201..416
                     /locus_tag="PLM83_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71865.1"
                     /translation="MAALLMIGSVIAVVILVVVLWFKRSADMSDAQWRHAAIARGFLL
                     VCGIAYLLIVIKTTTCTLREIFRVWPK"
     gene            482..1399
                     /locus_tag="PLM83_10470"
     CDS             482..1399
                     /locus_tag="PLM83_10470"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOK71866.1"
                     /translation="MKVAVLMGGSSSEREISLKSGAQVEAALRRLGHEVISVDPANNA
                     IGVLAELRPDVAFIALHGKYGEDGCIQGALELMGIPYTGSSVLSSAIAMDKTISKALF
                     EVSGIPTPPYVVVDCHSPFQSALERALSIGIPCVVKPSSQGSTVGISIVKDDGALQDA
                     LSYAFNYDEEAVVEKFIKGTEVTVGVLGNHDTEALPVLEIVPKNEFYDWESKYTPGMS
                     EHVIPARIGNAAQKECQRLALAAHAALKCRGYSRVDMIVEPNGTVWVLEVNTLPGLTE
                     VSLLPDAARARGISFDQLIQILLDGALKD"
     gene            1624..>2257
                     /locus_tag="PLM83_10475"
     CDS             1624..>2257
                     /locus_tag="PLM83_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOK71867.1"
                     /translation="MRDASIDLEHLSADLRVVLDSINEGIHIVDTCGITVFYNRIAAS
                     LDNLDPQEVVGRHILSVFPSLSEDTSTLMSVLRTGRPAPVRQQTFRNYKGDTITTMNS
                     TLPIYSGAKLIGAIEVSKDVTLVKELVERVVDLQAELITTAGVHSATGHRVKVGTARY
                     TFDDIVAVSQNMQDVVARAKRASQSGSAVLIYGETGTGKELVVQSIHNSSP"
BASE COUNT          534 a          588 c          605 g          530 t
ORIGIN      
        1 gtggacgacc atactattgg ctatccgggg acccggatac ccctccatcc gatgacggac
       61 tggatacgga agccatcaga accggagctg tttcaatcac tcccttaagg ttcgatgcaa
      121 cttgtcggcg tacagcggat atgcttaagg agtgggacat tcgtctgtag ggcggcatag
      181 tatgcatcgg tgatgaagct atggcggctt tgctcatgat cggatccgtt attgccgtgg
      241 ttattctcgt cgtggtgctc tggttcaagc ggagcgccga tatgagcgat gcccagtggc
      301 gtcatgctgc aatcgcccga ggatttctgc ttgtctgcgg aatcgcatac cttctcatag
      361 tgatcaagac gaccacctgc acattgcggg agatttttcg tgtctggccc aagtgatagt
      421 gtgcagtgtc ggtctacttc agctagaaac ggcggtgacg tcattggatg atcaaaccca
      481 gatgaaagta gctgtcctca tgggaggatc ttcgagtgag agagagatct cactcaaatc
      541 aggcgcacag gtagaggccg ccttgcgcag gctaggccat gaggtcatat cagtcgaccc
      601 ggcaaacaac gccatcggtg tgctggccga gctccgcccc gatgtggctt tcatcgcgtt
      661 gcatgggaaa tacggcgaag acggttgtat acaaggggcc ctcgagttga tgggcatccc
      721 atacaccggc tcatcggtgc tctccagcgc aatcgccatg gacaagacaa tctccaaggc
      781 gctctttgaa gtgtcaggaa tccctactcc cccctatgta gtggtggatt gccattcccc
      841 gttccaatcg gctttggagc gcgccttgag catcggcatt ccgtgtgtag tgaagccatc
      901 ttcgcagggg tcgactgtcg gaataagcat agttaaggat gacggcgctc ttcaagatgc
      961 attatcgtac gccttcaact atgatgaaga agcagtcgtc gaaaagttca tcaagggtac
     1021 ggaagttacc gtaggcgtcc taggtaacca tgatacagag gcgctgccgg tgcttgagat
     1081 agtgccaaag aacgagttct acgattggga atcgaagtat actcctggga tgtctgagca
     1141 cgtaatccct gctcgaattg gaaatgccgc acagaaagag tgccagaggc tcgctttggc
     1201 tgcccatgct gccttgaagt gtcgcggtta ctcgagagtc gacatgatag tcgaaccgaa
     1261 tggcactgta tgggttctcg aggtgaacac gctcccaggc ctcactgagg tgagccttct
     1321 gcctgacgcc gcccgggcgc gagggatttc cttcgatcaa ctcatccaga ttctgctcga
     1381 tggtgccctg aaagactgaa cgaaatatat ggagctttgg taagatgact cattggtgga
     1441 gccaagcgat ccaccaatga gtcatcttct ttcctcctag ctcacccata ggtcagtcat
     1501 acacccggat tcccaagttt cttctccctg attcatcctg ttgtttctgg ccaatgtacg
     1561 atacaatcgt agttggatgc cgaaagttgg gcatgcaaat ggttcctgcg gtggtggtga
     1621 ctgatgcgtg atgcttcaat cgatcttgaa cacctcagtg cggatctgag agtcgttctc
     1681 gattccatca atgaaggaat ccacatcgtt gacacatgtg gcatcacagt cttttacaac
     1741 aggattgcag cttccctcga caacctagac ccccaagaag ttgttggacg ccatattcta
     1801 tccgtctttc catcattatc agaagataca agcaccttga tgtcagtcct tcgtactggt
     1861 cgcccagccc ctgtaaggca gcagacattc cggaactaca agggtgacac catcacgaca
     1921 atgaactcaa cacttccgat ctacagcggt gccaagctca taggcgccat cgaggtctcc
     1981 aaagacgtta ccttggtgaa agagctagta gaacgcgtag ttgatctcca ggcggagctg
     2041 ataacaacag ctggcgtgca ctcggcgact ggccatcgag tcaaggtagg aacggccaga
     2101 tacacctttg atgatatagt cgctgtcagc cagaacatgc aggatgtcgt ggcccgtgca
     2161 aagcgagcat ctcagagcgg ttccgcagtg ctgatctacg gcgagaccgg aaccggaaaa
     2221 gaactcgtcg tacaatcgat ccacaactcc agcccac
//
LOCUS       DAOSAI010000388         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_31579_length_2250_cov_3.249658, whole genome shotgun sequence.
ACCESSION   DAOSAI010000388 DAOSAI010000000
VERSION     DAOSAI010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31579_length_2250_cov_3.249658"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLM83_10480"
     CDS             <1..917
                     /locus_tag="PLM83_10480"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOK71868.1"
                     /translation="VPGVRFLSTASVDSKRIEALVEELKTLEQDLTTADICCKRNVAD
                     QIASIRTRLLGLLADLLATGDDATRRAAAYGLSKLEDSRCIPLLADSLSDPDEGVRVW
                     AVEGLSSCGGNAALLPLVRALRDESVYVYYSACNALKSVDRDGLWPLIEAKLCSKDIT
                     VRKRAAKLVGDMGEDRAVPHLIALARDADRGVRYEAVEALRKIGDPASFDVLADCLED
                     DSRDVRIAAIQALRIIGDIRAVEPLVAICIENEDLRIPALQALKEIAGDEAVTPVIEA
                     LDKARELVSATAEQVMGIVAESHARVVS"
     gene            937..1623
                     /locus_tag="PLM83_10485"
     CDS             937..1623
                     /locus_tag="PLM83_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOK71869.1"
                     /translation="MDRALLWQQLGLMSLATLIGSVIGLERERQGKSAGLRTHSLVCL
                     GSALITIVSIHGFGPAGSGLHDPARVAAQVVSGIGFLGAGTIMREGLSIRGLTTAASL
                     LTTAGIGLAIGAGMVWPAIGATVLVQFILVIMSRVERKYFPHGEAVLKVNAIDRPGLL
                     GEIATIIGKYGMNIVETEINVDRENSIVSIDVTISSNMVGRDPQLAQADLLQHVLSLS
                     GILKAEIERF"
     gene            1664..>2250
                     /gene="surE"
                     /locus_tag="PLM83_10490"
     CDS             1664..>2250
                     /gene="surE"
                     /locus_tag="PLM83_10490"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOK71870.1"
                     /translation="MISNDDGIWAPGLLALARAIAEIADKLWICAPDRERSAISHALT
                     MGLPLRAEKTAFRGIEAEAWSVSGTPSDCVKLALEELLPERPDIVLSGINRGPNLGTD
                     VIYSGTVAAAAEGAFAGIPAIAVSTGSYDPSDYEPAARVAAKLASQVATRGLPEGVVL
                     NVNVPDGFNSGKVAVTRLGVQRYNNIFERRVDPRGR"
BASE COUNT          514 a          557 c          695 g          484 t
ORIGIN      
        1 atgtgccggg ggtgcgtttc ttgtctacag catcggttga ttccaagcga atagaggctc
       61 tagttgagga acttaaaacc ctggagcagg acttaacaac cgcggatatc tgttgtaaac
      121 ggaacgttgc tgatcagata gcttctatca ggacgcgatt gttgggactc ttggccgacc
      181 tcttggcaac cggggatgat gctacgcggc gagccgcagc ctacgggctt tccaagttgg
      241 aggactcaag atgcatccct ctgcttgcag attcgctttc agaccctgac gagggagtta
      301 gggtctgggc ggttgagggg ctgtcttcgt gcggcggcaa tgcggcgttg ttgccgctag
      361 tccgcgcatt acgcgacgag agcgtatacg tctactacag cgcgtgcaac gctctgaagt
      421 ctgtggatcg cgatggactg tggcccctga tcgaagcaaa actatgttct aaagacatca
      481 ccgtaagaaa acgcgcagct aaactcgtgg gcgatatggg cgaagaccgg gcagtgccgc
      541 acctgattgc gttggcgcgc gacgcagacc gcggcgtgag gtatgaagca gtggaggcat
      601 tgcgcaagat aggtgatcca gcttcattcg acgtcttggc agactgcctc gaggacgact
      661 cgcgcgatgt ccgaatcgca gcgatacaag cgcttcgaat aattggtgat atcagggcag
      721 tagaacctct agtcgccata tgcatagaaa acgaggactt gcgcattccc gccctgcaag
      781 cattgaagga gatagctggc gacgaagcgg ttactcccgt catagaggcg ttggacaagg
      841 cgcgcgaact agtgagcgca accgccgagc aagtaatggg cattgtagca gaatcccacg
      901 ctagagtggt aagctagagc gattggaggg gggagtatgg atagagcttt gctctggcag
      961 cagttagggc tgatgagcct cgcaacattg ataggctcag taatagggct tgagagggag
     1021 aggcaaggca agtccgccgg cttgcgcacg cactccttgg tatgccttgg gtcggccctt
     1081 atcaccatag tcagcataca tggttttggc cctgccggtt ccggtctgca cgatcctgcc
     1141 agagttgcgg cgcaagttgt cagcggcatt ggtttcttgg gagcgggcac gatcatgaga
     1201 gaaggcctct ccatcagagg gcttacaact gcagcaagcc ttttgacgac agcaggaata
     1261 ggccttgcca ttggagcggg aatggtgtgg ccggcaatag gcgcaacagt gttggtccag
     1321 ttcattttgg tgattatgag cagggtcgaa agaaaatact ttccacatgg agaagccgtg
     1381 ctcaaggtga acgccattga ccggccgggg ctacttggcg aaattgccac tataattgga
     1441 aaatacggaa tgaacatagt cgaaaccgag ataaacgttg acagggagaa ttctattgta
     1501 tcgatcgacg tcacaatctc gtccaacatg gttggcaggg atccccagtt ggcccaggca
     1561 gacttgcttc agcatgttct cagcctttct gggatactta aggccgagat cgagagattc
     1621 tagtcttggg tcacagtagg aggtctttcc cattctcaaa gcattgatct cgaacgatga
     1681 tggcatatgg gcgcctggat tgctggccct tgccagagcc atcgctgaaa tcgcagataa
     1741 gctctggatc tgcgcgccag accgagagag gagcgcgata agtcatgccc tgacaatggg
     1801 gcttccgctc cgggcggaga agactgcgtt taggggtatc gaggctgagg cgtggtcggt
     1861 cagcggcacg ccgtccgact gtgtcaagct ggccctagaa gagctcttgc cggaacgtcc
     1921 cgacatagtc ctctcaggca tcaaccgcgg gcctaacttg ggaaccgacg tcatatactc
     1981 tggcacagta gctgctgcag ccgaaggcgc gtttgccggc ataccggcga ttgcggtttc
     2041 cacaggatca tatgacccat cagactatga gccagcggct cgcgtagcgg cgaagctcgc
     2101 ctctcaagtg gccactaggg gcctgccaga aggggtggtg ctcaacgtga atgttccgga
     2161 tggcttcaac tcaggcaagg tggccgtcac caggctgggg gttcaacggt ataataacat
     2221 cttcgagcgt agggtagatc ctcgtggacg
//
LOCUS       DAOSAI010000389         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_31824_length_2238_cov_3.081997, whole genome shotgun sequence.
ACCESSION   DAOSAI010000389 DAOSAI010000000
VERSION     DAOSAI010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31824_length_2238_cov_3.081997"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PLM83_10495"
     CDS             <1..930
                     /locus_tag="PLM83_10495"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOK71871.1"
                     /translation="FPGEVSNTELLRRGAMSLADYYEAQAVKLGLDRNTAVRDALAKF
                     KFEVSKANDAESIAYIIGYYGRATGDVIEREQENRRREAVLGIVNSDPQVIAVTGQAM
                     ITVSAGQDGRIVVEDTAHVLSLSAIDAIQKSPVLTDLAAPIDIEIAGGKANVITPRTM
                     QDRVRLLRDETDSLRLSLDELRRAAGYSALSGSGILVSMYDAEGGFTSDEVVQDKDVR
                     DAVNELYAAGAQGIEVGGQRLVATSSIRSAGPQILVNQKPIPVNPVVIKAVGDPQVLE
                     SSLDLIKNSLKRWGIRVEVERYDNISLSPYRAQ"
     gene            893..2011
                     /locus_tag="PLM83_10500"
     CDS             893..2011
                     /locus_tag="PLM83_10500"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOK71872.1"
                     /translation="MTTLAYRRTAPNDQPVKWRKVRRPRVVRMGELDWLALLLLASIV
                     LLFDALVIGRYVGLIRLPGELTALDMARLGAMGYVDMQRTMLERDEFAGIGSAEPVLT
                     AAAQNLIAARNVEDIGRELAQAAADLHEAVQSDRLSSARGRVLELLSSDSGVQGYTGK
                     GAYILITPGASESQVQDRQNVLGAATREALSADPLIARSPTAIEVVIDQSGISFDTGD
                     LATQADLLAREIDSIHEEMDALREAAGLSELEGEGVIVLAYDAPSGYGFDEIVHQRDV
                     SEILDRLFAAGARGAQVGGERIISTSSIRCIGPIVLVNQRPVAVNPIRIYAVGNPYAL
                     ERALRPMASEFAKTGKRLEIKRESSLVLSAYHKGGLQW"
     gene            2032..>2238
                     /locus_tag="PLM83_10505"
     CDS             2032..>2238
                     /locus_tag="PLM83_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71873.1"
                     /translation="MLVVFLVITAGVAFIRFWHEAKHIGRGASPGSFFRQDEADQSIR
                     VDPLTGLPPLQDAEYFAAVIENAPA"
BASE COUNT          489 a          534 c          707 g          508 t
ORIGIN      
        1 tttccgggtg aggtctcgaa tacggaattg cttcgccgtg gggccatgtc ccttgccgat
       61 tactacgagg ctcaagcggt gaagcttggt cttgatagga atacggctgt gagggacgct
      121 cttgccaagt tcaagttcga ggtatcgaag gcgaatgacg cggagtccat cgcgtacatc
      181 atcggatact acggaagggc aacgggcgac gtcatagagc gtgagcagga gaaccgtagg
      241 cgcgaggcgg tactcggcat agtcaattcc gacccgcagg tgattgcggt tacgggtcag
      301 gcgatgataa ccgtctctgc aggacaggat ggaaggatcg tagttgagga taccgctcac
      361 gtcctatcgc tttcggcaat agacgcaatt cagaagagcc cagtcttaac tgatctagcc
      421 gcgccgatag acatcgaaat cgcgggtggc aaggcaaacg tcattactcc acgaacgatg
      481 caagataggg tgcgcctatt gcgcgacgag actgactccc tcaggctttc tctggatgag
      541 ttgaggcgcg ctgcgggata ttcggcgctg tcaggttcgg gtattctcgt gtcgatgtac
      601 gatgctgaag gtgggtttac aagtgacgaa gtagtgcagg ataaggatgt tcgcgacgct
      661 gtgaacgagc tgtatgctgc aggagcgcaa gggattgagg tcggcgggca gaggctcgtg
      721 gccacgtcgt cgatccgttc tgcaggtccg cagatccttg taaaccagaa gccgataccg
      781 gtcaacccag ttgtgatcaa ggctgtagga gatccgcagg tgcttgagag cagcctcgat
      841 ctgataaaaa actccctcaa gcgatggggg atcagagtgg aagtggagcg gtatgacaac
      901 attagcttat cgccgtaccg cgcccaatga tcaaccggtc aagtggcgaa aggtaagaag
      961 gccgagagtt gtcagaatgg gcgaactcga ttggcttgcc ctgcttttgc ttgcgtcgat
     1021 tgtgcttctg tttgacgctc ttgtcatagg acgatatgtt ggactcataa gactgcctgg
     1081 ggagttgacg gcgctcgaca tggccaggct tggtgccatg ggttacgtgg acatgcaaag
     1141 gacaatgctt gaaagggacg agtttgcggg gattgggagt gcagagccgg tcctgaccgc
     1201 tgcagcccag aatttgattg ctgcgcgcaa cgttgaggat atcgggcgtg agcttgcaca
     1261 ggctgctgct gatcttcacg aggccgttca gtcggatcgc ctttccagcg cgagagggcg
     1321 ggttctggag ttgctcagtt cggacagtgg ggttcaaggc tacacaggga agggtgcgta
     1381 catcctgata acccccgggg catccgaatc gcaagtccaa gataggcaga acgtcctggg
     1441 agccgccact cgcgaagctc tgagcgcgga tcctttgatt gccaggtcgc ctacggccat
     1501 cgaggtcgtg atcgaccagt ccggaatctc ctttgatacg ggcgatctcg caacccaagc
     1561 agatttgctt gcccgtgaga ttgactccat ccatgaagaa atggacgccc ttagagaggc
     1621 cgctggactt tctgagttgg agggtgaagg cgtgatagtc ttggcttatg atgctccttc
     1681 aggctacggt tttgacgaga tcgtccatca gagggatgtt tcagagattc tggataggct
     1741 ttttgcggca ggcgcccgtg gggcccaagt tgggggcgag cgaataatct ctacctcttc
     1801 aataagatgc ataggtccga tagttctggt aaaccagcgg ccggttgcag ttaacccaat
     1861 acgtatatac gctgttggaa acccatacgc gcttgagcgg gctcttaggc cgatggcgtc
     1921 ggagtttgcc aaaactggaa agcggctcga gataaagcgc gagtcaagcc tcgtcctttc
     1981 agcgtatcat aagggaggcc ttcagtggtg aggccccggc gatggcaaat cttgcttgtc
     2041 gtttttcttg tcatcactgc cggagtagcg tttataaggt tttggcatga ggcgaagcat
     2101 atcggaaggg gcgcatcgcc cgggtcgttt tttcgccagg acgaagcaga tcaatctatt
     2161 cgggtcgatc ctctgacagg cttgccgccg ttgcaggatg cagagtactt tgccgcggtc
     2221 atcgaaaatg cgccggct
//
LOCUS       DAOSAI010000390         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_32116_length_2223_cov_3.509225, whole genome shotgun sequence.
ACCESSION   DAOSAI010000390 DAOSAI010000000
VERSION     DAOSAI010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32116_length_2223_cov_3.509225"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLM83_10510"
     CDS             <1..325
                     /locus_tag="PLM83_10510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71874.1"
                     /translation="AGKSPGAMAEAVGENFDFLLVANAQSNEVTIIDIETHRVVAVVA
                     VGQEPGDITITPDGQYALVLNRVSGDMAVIRLGAITATRSRSAPLFTMIPVGSKPVGA
                     VVRGV"
     gene            complement(656..991)
                     /locus_tag="PLM83_10515"
     CDS             complement(656..991)
                     /locus_tag="PLM83_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71875.1"
                     /translation="MALTEEQLDNLTAQFNLRVVLDPETREEKTFTNFSKAITFFKHE
                     LEFWQAQSGGLTDIISKYQSVLSSLEAAKDQPDENQARRLYPRQLTDCGNAPHYRLWN
                     RVWSVSRQA"
     gene            complement(1010..1483)
                     /locus_tag="PLM83_10520"
     CDS             complement(1010..1483)
                     /locus_tag="PLM83_10520"
                     /inference="COORDINATES: protein motif:HMM:NF021590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2321 domain-containing protein"
                     /protein_id="HOK71876.1"
                     /translation="MQGYDVAQICLNGHVTNSSIRDFPHHNQEYCNICGTKTVTACQG
                     CQKKIRGRLRGVLTIEPMSAPAFCQHCGKPFPWTEARIEAARDLADQLGLDVPDRTLL
                     EKSIDELLRDSPRAPAEAVRFRTILCRAKPWGLEAFKQVLGMILSEGVKKLIWPA"
     gene            complement(1893..>2223)
                     /locus_tag="PLM83_10525"
     CDS             complement(1893..>2223)
                     /locus_tag="PLM83_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71877.1"
                     /translation="RDKSGVYEVKLEAKIPLKASSWLAARVAEPSRQGKTIMPRRMTV
                     FAHTNPVYFLRDGVKVRVQKAIDYLRLYLRYSERWFQTGAKFDSEATKQQALEQVKWA
                     VDYYSKL"
BASE COUNT          453 a          635 c          601 g          534 t
ORIGIN      
        1 ggccggcaag tctcccggag caatggccga ggcagttggc gagaactttg atttcctgct
       61 ggtggcaaac gcccagtcga acgaggtcac gattatcgat atcgaaactc accgcgtggt
      121 ggcggtcgtg gcggtaggac aggagccggg cgacattacg atcacccccg acgggcagta
      181 tgcgctcgtg ctgaaccggg tttcgggaga catggcggtg atccgcctgg gagcgatcac
      241 ggcaacgcgg agccggtccg ccccgctgtt cacgatgatt ccggtgggct cgaaaccggt
      301 cggcgccgtc gtgcgcgggg tgtgaatctc gtttgaccca gtgcggcatc gatttgtgcg
      361 atagattccc tgcagtgacg cttcgctcgt cgccttccag gtctcaacgt ccctcttcgc
      421 acattcatag ctgtctgcca gcttctcggc caatgcagat acctcagaac tcttcttatc
      481 gaatgcctga atacgtgctt ccgttcccag tgcaaagact tcagggtgcc tgaaggcgat
      541 tagtgccatg agcccgaaca cagactcgcg cggcttgttt atccaacctt gattgtggtt
      601 gtatatgccc gcatggaact caactgctgc tttcgctgcc tggggatttg cctggttaag
      661 cctgacgaga aactgaccaa acgcggttcc agagcctata atgaggcgcg ttgccgcaat
      721 ctgttagctg cctcggatat aagcgacggg cctggttttc atcaggctgg tccttagcag
      781 cctcaagcga actgaggaca ctttgatatt tgctgattat gtcggtaaga ccaccgctct
      841 gcgcttgcca gaactcaagt tcgtgcttga aaaatgttat tgccttagaa aagttcgtga
      901 aggtcttctc ttcgcgagtc tctgggtcga gcacaactct caagttgaat tgtgctgtaa
      961 ggttatccaa ctgttcttcg gtgagtgcca tcgcatgctg cccctttcgc taggccggcc
     1021 aaatcaactt cttcacgccc tcgctaagaa tcattccaag cacctgtttg aacgcctcca
     1081 ggccccaagg cttagccctg catagaattg ttcggaatcg gactgcctcc gcaggggccc
     1141 tcggagaatc ccggaggagc tcgtcgatgc tcttttcaag cagcgtacgg tcgggaacat
     1201 ccagtccaag ctggtctgct aagtcacggg cagcttcgat tctcgcttca gtccatggaa
     1261 acggctttcc gcaatgctgg caaaatgccg gtgcggacat tggttcgatt gtgagcacac
     1321 ctcgaagtcg tcctctaatc tttttctgac agccttggca cgccgtaact gtctttgttc
     1381 cgcagatatt gcaatattcc tggttgtggt ggggaaagtc acgaattgaa ctattcgtca
     1441 cgtgtccatt caggcaaatc tgtgcaacat cgtagccttg cactgaagca gacctccttc
     1501 tagtcgggat cacggttcac tgccatccca tcgcatggcg gaaggggatt atgtcaaagg
     1561 gtttcgatgt tgcgaattgc cccatctaaa tgatttttcg ggctccgaga cacaggtaac
     1621 aaaacgtact gggtgaacaa gttatttttg tcccgcaaaa aggggcgaag aagaccgcgc
     1681 catagaggga gagttctgtc gtggacgaac ggatgcgtta tacgattctg ctcaaggatc
     1741 gcgagagcat ggctgccctg aacaagcgcc gaaggaacag agccgccgga gggcctgatc
     1801 cgaacgatct ttggtgtgtg gattacaaac gcaggcgggc tggtggcgcg gatcctacgg
     1861 tacgtcacct tgccgacgtg ccagccccct cgctacaact tactgtagta atcgaccgcc
     1921 cacttcacct gctcgagcgc ctgctgcttt gtcgcctcgc tatcgaattt cgcccccgtc
     1981 tggaaccacc gctcgctata tcgcaggtac agcctcaggt aatcgatcgc cttctgcacc
     2041 cttactttga ccccgtcccg cagaaagtag accggattgg tatgcgcgaa caccgtcatc
     2101 cggcgcggca tgattgtctt gccttgccgg cttggctccg ccacccgcgc cgccagccac
     2161 gagctcgcct tcaatggtat cttcgcctcc agcttcactt cgtagacgcc tgacttatct
     2221 cgg
//
LOCUS       DAOSAI010000391         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_32335_length_2213_cov_3.743281, whole genome shotgun sequence.
ACCESSION   DAOSAI010000391 DAOSAI010000000
VERSION     DAOSAI010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32335_length_2213_cov_3.743281"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PLM83_10530"
     CDS             <1..930
                     /locus_tag="PLM83_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOK71878.1"
                     /translation="DITSSAPGARLFPRVVDPWEAIWEVTREAADETGLPSGIPVAAG
                     SIDAFVEALGAGVTSPGLICDSTGTSTCVSAVAPDGCSLQTVKHVIPGERLIVSPISY
                     SGGCLAWAMSVLYPEVYSSKLDWRDTVAEALDCSESGASGLVFLPYFVGKRSPVAVPR
                     ATGAFLGLRPEHDSCHMIRAVLEGCAYAMRQSMEAWQDSMKAVDVRAVGNGARHRGWL
                     QMKADVLNLPVASMKVKEGTLLGAAILAGTAANVFSSIKEAVQVVVLTDAVFTPEGVR
                     GDGYDRGYAAFCRAESALQEFWNSAEPAWPAQH"
     gene            946..2127
                     /locus_tag="PLM83_10535"
     CDS             946..2127
                     /locus_tag="PLM83_10535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK71879.1"
                     /translation="MESQASRTPKGIQLIAAGMLLWGFGYGLHSYMFPVYLRNIGCTP
                     QEVGFVYSISMAVMAASSIPGGMLADKYDRKRVILLSWIMGVPSVILYYFAKDWRLAA
                     LGIALYSGSMLGYPALNAYAGACSPEGESGRTFGLISAGFSGGMIASPILSAYLSSIW
                     PIENLFLLSFAFFAAACCVIAFLPPEPESESTASVSTYASLLADRSFMKFVFCYSLCA
                     FSFYMVQQIIPQYLSDVRHTNISIVSLLGSIMSLGQTMITVVVGRMADNRGTLVAVGA
                     NVIVFVISMLCFIAISGQVAVVICLFLLGGFMAGHGVAFAGVGEVLGDGADGKAFALF
                     NLATWGASIVGPYLGGFFYSTSENTPFYIAALLLVVGGTMLIRQGSTIHVVGEVRKGQ
                     G"
BASE COUNT          459 a          516 c          676 g          562 t
ORIGIN      
        1 gatattacct catctgcccc cggagccagg ctgtttccga gggtggtgga tccgtgggag
       61 gccatttggg aggtaacccg cgaggctgca gacgagacgg ggcttccctc aggaataccg
      121 gttgctgcgg gaagcatcga cgcgttcgtc gaggctctgg gcgcaggggt gactagtcct
      181 gggctaatat gcgactcaac cggcacgtca acatgcgttt ctgcagtggc tcctgatgga
      241 tgcagcctac agactgtaaa acatgtcatt cccggagagc gattgatagt ttccccaata
      301 tcctacagtg gtggatgcct tgcctgggcc atgtctgttc tctacccaga agtgtactca
      361 agcaagctcg actggaggga cacggtcgct gaagcgctcg attgctcaga atctggagcg
      421 tctggcttgg tgtttctgcc atatttcgtc ggcaaacgca gccccgttgc ggttccaagg
      481 gcgacaggcg cttttcttgg actgcgaccg gagcatgatt cttgccacat gatccgtgct
      541 gttttggaag gctgtgccta tgccatgcgc cagagcatgg aggcgtggca ggattcgatg
      601 aaagcggtgg atgttagggc tgtagggaac ggcgcaaggc accgaggttg gttgcagatg
      661 aaggctgatg tgctcaatct gccggtggca agcatgaagg tgaaggaagg cacacttctg
      721 ggcgcagcga ttctggcagg aaccgctgca aatgtattca gcagcatcaa agaggctgtt
      781 caggttgtag ttctcacgga cgctgtattc accccggagg gcgtgcgcgg tgatggatat
      841 gaccgtggtt acgcggcgtt ttgtagggcg gaaagtgcat tgcaggagtt ctggaatagt
      901 gcagaaccag cctggcctgc gcagcattga tcggaggtaa cgttggtgga gtcgcaagcg
      961 agtcgtacgc cgaagggcat acaattaata gctgcaggca tgttgctttg gggattcgga
     1021 tatggtcttc attcatacat gtttcctgtg tatctccgca acattggatg caccccacag
     1081 gaagtgggat tcgtctactc aatatccatg gcggttatgg cggcgtcgag cataccaggg
     1141 ggaatgctgg ccgacaagta tgaccgaaaa agagtgatcc ttttgtcgtg gattatgggc
     1201 gtgccatctg taatattgta ctatttcgcg aaagactgga gacttgctgc tcttggcatt
     1261 gctctttact cagggtcgat gttgggctac cccgcactca acgcatatgc tggggcttgt
     1321 tctcccgaag gcgagtcggg ccgaacgttt gggctgataa gcgcaggatt ttcaggggga
     1381 atgatagcga gcccgattct atccgcttat ctgtctagca tttggcccat cgagaatctg
     1441 ttccttcttt cgttcgcgtt ctttgcagca gcatgttgcg tgatagcgtt cctgccacca
     1501 gaacccgaaa gtgagagcac tgcaagcgtt tctacatatg caagtctgct ggccgaccga
     1561 tcgttcatga agttcgtatt ctgctactct ctgtgtgctt tctcttttta catggtgcag
     1621 cagataattc cgcagtatct cagcgacgtg cgccacacaa acataagcat tgtcagtctg
     1681 cttggttcga taatgtctct tgggcaaaca atgataactg tcgtggtagg gcgaatggca
     1741 gacaatcggg ggactcttgt agcggtagga gccaatgtga ttgtctttgt catctcgatg
     1801 ctgtgtttca tcgccatttc aggccaggtt gctgtggtca tatgcctctt ccttctgggc
     1861 ggattcatgg ctggccatgg cgttgccttt gcaggggtgg gggaggtcct cggcgatggc
     1921 gcagacggaa aggcttttgc cctcttcaat ttggcaactt ggggggcgtc gattgtagga
     1981 ccctacctgg gtggcttttt ctattccaca tcggaaaaca cgccattcta cattgcagct
     2041 ctcttgttgg tagtgggagg aacgatgctg atcaggcagg ggtctaccat acatgttgtc
     2101 ggggaggtgc gtaaggggca aggatagccg tgggaacttg ggtcaaggaa tcttgcgatc
     2161 aactgaaatg gcgttgcgcc aaaaccatat cgaaagggag agacgaaatg cgc
//
LOCUS       DAOSAI010000392         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_32349_length_2213_cov_2.886006, whole genome shotgun sequence.
ACCESSION   DAOSAI010000392 DAOSAI010000000
VERSION     DAOSAI010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32349_length_2213_cov_2.886006"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /gene="infB"
                     /locus_tag="PLM83_10540"
     CDS             complement(<1..1552)
                     /gene="infB"
                     /locus_tag="PLM83_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOK71880.1"
                     /translation="MRTGDIEDIDEIAVKRGGRKREKAPKVRRIEVGETISVRELAAK
                     LEISASDCLRRLMKLGVMVRINDEIEGEVAQILASELGFEAELTSEREERELVIAEIE
                     DPPESLKPRPPVVTIMGHVDHGKTSLLDAIREANVTATEAGGITQHIGAYQVELDGRK
                     ITFLDTPGHEAFTAMRARGAHATDVAVLVVAADDGVMPQTVEAINHAKDAKVPIVVAV
                     NKIDKPDANPERVATQLSERGLVPEKWGGDTVMVPVSALHKQGIEDLLEMILLIADMS
                     ELKANPDRPAMGIVIEAKVDKGRGPVATVLIQKGTLRVGDYFLVGQVHGRVRAMTNYK
                     GEPVKEATPSMPVEVLGLGDVPLAGDVFQVVVDEREAKQVAARRAERRRIETIGEAPK
                     KVSLEELFSQIQEGEVKELRVIIKADVQGSVEAVRQAIEQLSTDEVRVDVIHGGVGAI
                     SESDIMLASASNAVVIGFNVRPDPVASMCAEDEKVDVRLYRVIYDAIDDVKKALEGLL
                     EPEYREVVY"
     gene            complement(1648..2109)
                     /locus_tag="PLM83_10545"
     CDS             complement(1648..2109)
                     /locus_tag="PLM83_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71881.1"
                     /translation="MPSRKIGSLRNRRNRLSQPESRSNLPRVPRGALGKRRRFNVNLA
                     GAGHILKRRRAQLVRASLRVLGRRAGQPPHIVMIAEIGEGIAALCATGPGFLEMRLML
                     AGVRTVNVASAHPLSNSHVLHKGPLLNNRVRVGNAHLRVDVVGARITLMMN"
BASE COUNT          399 a          747 c          546 g          521 t
ORIGIN      
        1 catacacgac ctcgcggtat tcgggctcca gcaatccctc gagggccttc ttcacgtcgt
       61 caatggcgtc gtatatgacc ctgtaaagcc tgacatcgac cttctcgtcc tctgcacaca
      121 tggaagctac cggatcaggc ctgacattga agccgattac gactgcatta gatgcagagg
      181 ccaacatgat gtcggactcg ctgatagctc cgacgcctcc gtggatcaca tcgacacgca
      241 cctcatcggt ggaaagctgc tcgatcgcct gccgaaccgc ctcaacagat ccctgaacat
      301 ccgccttgat aatcactctg agttctttga cttccccttc ttggatctga ctgaacagtt
      361 cttcaagaga taccttcttg ggagcttcgc caatggtctc aatgcgccgc cgttctgccc
      421 gtctagcggc gacctgttta gcctcgcgct catccacaac gacttggaac acgtcgcccg
      481 ccagcggaac atcgcccaag cccagaactt caactggcat ggatggcgtg gcctccttca
      541 caggctcgcc cttgtagttc gtcatggctc tgactctacc atgcacttgg ccgacgagga
      601 agtagtcgcc caccctgagc gtccccttct ggatcaacac agtagcgacc ggtccacgtc
      661 ccttgtcaac cttggcctcg atgacgattc ccatggctgg cctgtcagga ttagccttca
      721 gttcactcat gtccgcgatc agaaggatca tctcgaggag atcctcaatg ccctgcttat
      781 gcaaggccga tacagggacc atcactgtat ccccgcccca tttttccggc acaagcccgc
      841 gttcggacag ctgcgtcgcc acccgctcag gattggcatc gggcttgtcg atcttgttca
      901 ccgcaaccac tatcggcacc ttggcatctt tagcgtggtt tatcgcctcg acagtctggg
      961 gcattacacc gtcatctgcg gctacgacca gcaccgcaac gtcagtcgca tgcgcgccac
     1021 gagcccgcat ggccgtaaaa gcctcgtgcc caggcgtatc caagaaggtt atcttcctgc
     1081 cgtcaagttc cacctggtat gcgcctatat gctgtgttat cccgcctgcc tccgttgcag
     1141 tcacattggc ctctcgtatg gcgtcgagaa gagaagtctt gccatggtca acatggccca
     1201 ttatggttac caccgggggc cttggtttca agctttccgg aggatcctct atctcagcta
     1261 tgacaagttc gcgttcctcc ctttcggagg taagctccgc ctcaaagcca agctctgacg
     1321 cgagtatctg cgctacttcg ccttcaatct cgtcattgat ccgaaccatc acgccaagct
     1381 tcatgagtct gcgcagacaa tcactggccg atatctcaag tttggcagcg agttcacgaa
     1441 cgctgatagt ctcccccacc tcgatacgcc tgaccttcgg cgccttctcg cgcttgcggc
     1501 ctccccgctt cacggcgatc tcgtctatgt cttcgatatc ccctgtcctc actcgatgct
     1561 cgagcaccct cgcatcttca agcggcttcg aagtaggcgg ctttttgcgg ccacgccgat
     1621 ctcccttgcg ctcggcacgt cgcggtccta gttcatcatc aacgtaatcc tcgcgccgac
     1681 cacgtccacc ctcagatggg cgttgcctac ccttaccctg ttgtttagca ggggaccctt
     1741 gtggagcacg tggctgttgc tgagcggatg cgccgaggcc acgttcactg ttctgacgcc
     1801 cgccagcatg agacgcatct ccaggaagcc tggaccggtc gcgcacaggg ccgctattcc
     1861 ctccccgatc tctgcgatca tcacgatatg ggggggctgc cccgccctcc tgccgagcac
     1921 cctgaggctt gcccgcacca gctgggcgcg acgccgcttg aggatatgcc cggcccccgc
     1981 caggttgacg ttgaaccttc tcctcttgcc tagcgctccc ctgggcactc gggggagatt
     2041 gctccggctt tctggctggc tcagcctgtt gcgccgattc ctcagacttc cgatctttct
     2101 gctgggcatc ggctttggcg cctggcgcgt cgggaccata cttgcgcatc acatatcgtg
     2161 ccgcattctc atcgatagaa ctcatatggt tcttggcctt gacgcccaac tcg
//
LOCUS       DAOSAI010000393         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_32472_length_2207_cov_3.843866, whole genome shotgun sequence.
ACCESSION   DAOSAI010000393 DAOSAI010000000
VERSION     DAOSAI010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32472_length_2207_cov_3.843866"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="PLM83_10550"
     CDS             complement(<1..1710)
                     /locus_tag="PLM83_10550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOK71882.1"
                     /translation="MPRYRFTWDAFDDATVIAVAGACGFDPGMVTPGDVADSARDWLT
                     LNWARPNEELVRRAKRELERTWLPDYPFLKTLVIELIESGLGPMGQPKSRSEQLAYVA
                     KCRNTTNFRRILANHMRMYGEGWSEDEVGNSIIRSWAVIDPSKQLPDSSKPFPYQVEA
                     WNRLSACSARKDSPGGFPGLLVMPTGSGKTYTAVHWLMENVVARGGRVMWVAHREGLL
                     QQAWESFVRLSALADNPAREDPLVIRIVSGSHCVVSQLTPDDDIMLCSVASLARHKAV
                     VEDILSDPDIFLVIDEAHHAPAKSYKDIIRYMKELKHNKLLGLTATPTRMIESERAVL
                     SQLFNNNIIYQAEFRDLVEQNYLARPIKIRVSTDIEAEAGMTEEERRHIATFDNLSEE
                     TLDRLGAMNERNDLIVSHYLGNEAKYGKTLVFATCINHAHLLRDKFRANGVNAEYVAS
                     RRLDEEAVNEDEVKAEFSSSDSGLDVLINVQKMTEGTDLPSVQTVFLARPTNSAVLMQ
                     QMVGRALRGPKVGGTPLAYLVSFEDHWEQFTDFERPFDLIPELVDPFEEAAPDIMEAD
                     EDIV"
BASE COUNT          483 a          591 c          596 g          537 t
ORIGIN      
        1 cacaatatcc tcgtccgctt ccatgatatc cggagcggcc tcttcaaatg gatctacaag
       61 ctcggggatg aggtcgaatg ggcgctcaaa gtcggtgaac tgttcccaat ggtcctcgaa
      121 tgacacgagg tatgccaggg gtgttccccc taccttcggg ccccgcagcg cccgccctac
      181 catctgctgc ataagcactg cggagttggt gggacgcgca agaaagaccg tctgaacgct
      241 tggaaggtcg gtcccctctg tcatcttctg gacgttgatg aggacgtcga ggccgctgtc
      301 cgatgagctg aattcggctt tcacctcgtc ctcattgacg gcttcttcat caagccgcct
      361 gctcgcaacg tattcggcgt tcacgccgtt ggcacgaaat ttgtccctca gcaaatgggc
      421 atggttaatg catgttgcga acaccagggt cttgccgtac ttcgcctcgt tgccgaggta
      481 atggctgaca atgaggtcgt ttctctcgtt catcgcgcct aacctatcca gagtctcctc
      541 gctgaggtta tcgaacgtgg ctatatggcg tcgttcttcc tcggtcatgc cggcttctgc
      601 ctcgatgtca gtggagacgc ggatcttgat aggacgggca agataattct gttccaccaa
      661 atctctgaac tcggcctgat agatgatgtt gttgttgaat agctgcgaca gcactgccct
      721 ctctgattcg atcatccggg tcggcgtggc cgtaaggccc agaagcttgt tgtgcttcag
      781 ttccttcatg taccggatga tgtctttgta gctcttggct ggagcatggt gggcctcatc
      841 gatgaccagg aaaatgtctg gatcgctcaa gatatcttcc acaacagcct tatgccgggc
      901 aaggctcgcc acagaacaca acatgatgtc gtcgtcaggg gtgagttgac tcacaacaca
      961 gtgactgcct gacacgatgc gaatgacgag cgggtcctcc cgcgcggggt tatcggcaag
     1021 ggccgaaagg cgcacaaacg attcccaagc ctgttggaga aggccctctc ggtgggcgac
     1081 ccacatcacc cgtccgccgc gggccaccac gttctccatg agccagtgca ctgcggtata
     1141 tgtcttgccg gatcctgtag gcatcacaag gaggccggga aaccctccgg gcgaatcctt
     1201 ccgggcactg caagcagaga gcctgttcca cgcctccacc tggtacggga agggcttgct
     1261 ggaatcagga agctgctttg agggatcaat caccgcccaa ctgcggataa tgctatttcc
     1321 gacctcatcc tcagaccatc cctcgccgta cattctcata tgattagcga gtattcgacg
     1381 gaagtttgtg gtgttgcggc acttggcgac atatgctagt tgttccgacc ggcttttagg
     1441 ctggcccatt gggccaaggc ccgattctat caattcgatg acgagggtct tgagaaacgg
     1501 atagtcagga agccaagtcc gctcgagctc tctcttggcg cggcgcacga gttcctcgtt
     1561 ggggcgagcc caattgagtg tgagccagtc gcgagcggaa tcggcgacat cccctggcgt
     1621 gaccattcca ggatcgaatc cacatgctcc tgctacagca atgacagtgg catcatcaaa
     1681 agcatcccac gtgaagcgat atctgggcaa ggcaatgact cccttcagat ctatggatgc
     1741 attatctcag atgggtgata atttgaggtt tccaagtgag tttgtagaag attgattagt
     1801 agtattatta gacattcttt tgttatggcc ttcccggttt ggcttgagga atcgctacag
     1861 cagcagattg aggcgccagc acctacgata gttagagtct caaaaggatt ttggggtatt
     1921 ggcggaacag ccgccatata attctcttcg ggtgtaatac aaaaacaagc cgaggaggta
     1981 accacgatgg cagatgtcaa gagaaacata tcatttttgg caccaggatc acgtcctaaa
     2041 tgtgctgtaa aaacggttct aggtcgttgg agcatacgca tacagatgcc tagtcgggac
     2101 taccgtcgaa tttcatctag ttcctcttcc tggctccggt gtacgcattc gccgggttcg
     2161 gactttctat ccaaaccaaa tgagtattgt actccagcga ggcttca
//
LOCUS       DAOSAI010000394         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_32824_length_2190_cov_2.940515, whole genome shotgun sequence.
ACCESSION   DAOSAI010000394 DAOSAI010000000
VERSION     DAOSAI010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32824_length_2190_cov_2.940515"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..>2190
                     /locus_tag="PLM83_10555"
     CDS             280..>2190
                     /locus_tag="PLM83_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71883.1"
                     /translation="MPLGRLQTVLLPDQFTIDVPWDSPGDSPNIEEGSHEKSDIAGQL
                     SEAMLHQVPGAIACLPRSVSGRDIPALIEYAKQSNTLIALPRPAIEDYRRLQRRNMTL
                     KCVNTWLHNDSVIDWNQASTWLIASREPGPAASYIARWYFAGGRMNAELHPLAAAWVS
                     QALPRECFIKPVPNGISAPLIDHAAILANPPRRGRRAVILDAEQLPDCVPEAAGERTE
                     IDSPDNVVSACALRFVSDRTVWNPATGDGRIRIAVTPEDSQDPVFEDFLAVLGRCTHP
                     NSREAVALLNRIDNADEVTILEVLADADGAKTILVMRTVSHADAWLADRISDLGNSDP
                     PAAIFAGPEAGTLLGDCLQKVAGCPVVPFAHSDDGCHSPGIGVCEDGASGTLTGLPRA
                     LQSAARRMVTQFDIAGQGLDAFCGCSPETAEVMVAALRAGGYRASSFTRFGSRTRTVE
                     RLSPEIPGLFIADSRPEPLPGYIRRAIIRRLPFPSRTDPEILAALACVDSDEDVFANV
                     ILPKMLRDLLRTITTLRAMGVEPVLLDRRALTHTSYRAHIEALVGPIVSIPGIPQEKE
                     PPGMDAVRSALQDGLHRIGVHGDFSLTRGIDPTPVLRRFYGSQAQWLPHQREVVDSLL
                     AGEDILAVMPTGS"
BASE COUNT          470 a          605 c          631 g          484 t
ORIGIN      
        1 gacctgcctg ccctcaagga tcagggggtt gccattcccg aggttagcat catcgacacg
       61 ctggaactct cgttggtcac cgagcctatg agatagcggc atgcccttga tggccagcac
      121 actgcggagg ccgatgtaaa ggacaatatt gatcttttca agcagctcga caagttttgg
      181 ttgagtgtaa gtgcagacga gctgcgatgg catatccagt ggaactccgc cgaaacaaga
      241 ctcggcaggt attatcggtg ggttgccgca aggaaagggg tgcctctagg ccgcctgcag
      301 actgtactgc tcccggatca gtttacaatc gatgtgccgt gggacagccc gggtgattca
      361 cccaacatcg aagagggctc gcatgagaag tctgacattg ccggacaatt gtcagaagca
      421 atgctccatc aagtacccgg agccatcgcc tgcctgccca gatcagtatc tggacgtgac
      481 attccggctt tgattgaata tgccaaacag agcaacaccc tcattgctct tccgcgcccc
      541 gcgatcgagg actacaggcg tctacagcgc cggaatatga cactgaagtg cgtgaacact
      601 tggctgcata atgactccgt aatcgactgg aaccaggcct ctacatggct gattgcatct
      661 cgcgagccag ggcctgcagc tagttatatc gcacgatggt acttcgccgg aggccgcatg
      721 aacgcggaac tccatcctct ggcagcagcg tgggtttcgc aagccttacc ccgcgaatgt
      781 tttatcaagc ccgttcctaa cggaatttcg gctcctttga tcgaccatgc tgcgatactt
      841 gctaaccctc caagacgtgg tcgaagggct gtcatactcg acgccgaaca gcttcccgat
      901 tgcgtgcctg aagctgccgg cgagagaacc gagatcgatt cgccggataa cgtggtctcg
      961 gcctgtgctt tgagatttgt ttcagacaga accgtatgga atccggccac aggcgacggc
     1021 agaatccgca tcgcagtcac tcccgaagat tcacaagacc cagtcttcga ggacttcctg
     1081 gcggtcttgg gcagatgcac acaccctaat tcgcgagagg cggtggcgct tcttaacagg
     1141 attgacaatg cagatgaggt caccatcctc gaagtattgg cagatgctga tggggcaaag
     1201 accatattgg taatgaggac tgtatcccac gccgatgcat ggcttgcgga tcggatttcc
     1261 gatcttggca attcggatcc gccggctgca atattcgccg gtcccgaggc aggcacctta
     1321 ttaggcgact gtctgcagaa ggttgcgggt tgtcctgttg tgccattcgc acattcggat
     1381 gatggatgcc acagtcccgg cattggagtc tgcgaagatg gcgccagtgg caccctgaca
     1441 gggcttccac gggctttgca gtcggcggcg aggcggatgg tgacacagtt cgacatcgct
     1501 ggccaagggt tagatgcgtt ttgcgggtgc tcgccggaga ctgcggaagt aatggtggct
     1561 gcgcttagag ccgggggcta ccgggcatct tcatttacca ggtttggatc gcgcacgcgt
     1621 accgtggaac gattatcacc ggaaatcccc gggctgttca ttgccgactc ccgtcctgaa
     1681 cctttgcccg gctatatcag gcgtgcgata atccggcgtt tgcctttccc atcccgaact
     1741 gatcccgaaa tcctggctgc cttggcatgc gtcgattctg atgaagacgt tttcgccaac
     1801 gtgatactcc ctaagatgct gagggatcta ttgcgcacaa ttacaacatt gagggccatg
     1861 ggtgtcgaac ccgtgcttct tgacaggcgg gcgctgactc atacatccta tagagctcac
     1921 atagaagcct tagttggtcc gatcgtatcg atcccaggga tcccgcagga gaaagaaccg
     1981 cccgggatgg atgcggtgag gtcggcccta caagatggcc ttcacaggat cggagtgcat
     2041 ggagacttct cgttgacccg gggcattgat cccacgccgg tcttgcgacg gttctatggc
     2101 agccaagcgc agtggcttcc gcaccaacgc gaggtagtgg actccctctt ggccggagag
     2161 gacatcttgg cggtgatgcc cacaggatct
//
LOCUS       DAOSAI010000395         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_33010_length_2181_cov_3.128881, whole genome shotgun sequence.
ACCESSION   DAOSAI010000395 DAOSAI010000000
VERSION     DAOSAI010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33010_length_2181_cov_3.128881"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLM83_10560"
     CDS             complement(<1..616)
                     /locus_tag="PLM83_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOK71884.1"
                     /translation="MVIHVVAPGETLWVISRRYGVSIAAIVSANAIADPNVLVVGEAL
                     AIPLPRPEPAPLTYVVRRGDTLAAIAGLFSTTVQSIALANQITDVNLVYVGQELVVPG
                     WTYQTYTVRSGDPLSRVAAAFSTSVQLIVAVNHIADPNVINVGQVLIIPARGLPAPPK
                     PLVETNGYIFLLSESTLRRILTPIAHDLTYISVFSFSVDGRGGLD"
     gene            813..980
                     /locus_tag="PLM83_10565"
     CDS             813..980
                     /locus_tag="PLM83_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71885.1"
                     /translation="MTKADKKTQLFAVVLVGAEEGDIENPLVRATSSADAISAALHDP
                     RWEGYACTFGS"
     gene            1222..1962
                     /locus_tag="PLM83_10570"
     CDS             1222..1962
                     /locus_tag="PLM83_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOK71886.1"
                     /translation="MRFLIDPEVFEVLPTMCIGVVVAHGIDNTQLQSDNAQKISALLS
                     KAMRRTRAKFAGTNVKEHPDIVPYREAFRALGYNPNKFPCSVEALTSRVVKGSDLPDI
                     NSVVNLVNAVSLELALPMGAHDLDSISGDLAVRFSRQGELFTPLGEAEPEEVPAGELV
                     YADGREIRTRRWIWRQNDRGKVTEESRNIFLPIDGFTDRNRDAVERAMAELAGRLEEF
                     FGVAAHQYFLDRDRNSCDLMASVEQKKA"
BASE COUNT          541 a          571 c          606 g          463 t
ORIGIN      
        1 aatcaagacc accccgccca tctacggaga agctgaaaac gctaatgtac gtgaggtcat
       61 gggcgatggg cgtcaggatg cggcgcaaag tgctttcgga aaggaggaag atgtagccgt
      121 tggtctccac gaggggcttg ggcggagcag gcaaccctct ggcgggtatg atcagaacct
      181 ggccgacatt gatgacatta gggtcagcta tgtggttgac tgccacaatc aactgaacgc
      241 tggtggaaaa ggccgcagcc acgcgtgata gagggtcgcc ggacctcaca gtgtatgttt
      301 gatatgtcca tccaggcacc acaagctcct gtccaacata gaccaggttg acatccgtta
      361 tttgattggc caaagctatg gactggacag tagttgagaa gagcccggca atcgcagcca
      421 atgtatcgcc acgcctcact acatacgtaa gaggcgcagg ctcagggcgc ggcagcggta
      481 tagcaagcgc ctcgcccacc actagtacat tcggatcggc gatggcattt gccgagacga
      541 ttgcagctat agacacacca tatctgcgcg aaatcaccca gagagtctct ccgggtgcca
      601 caacatggat caccatacaa atcactccca tgggaaatca gtcatcccat catatggctc
      661 tcctgcagca tttgcgtttt cgaccgttcg tcgtcctaat ctcgcgccgt tgataagtgt
      721 agctgcggtt cgaattcttc gggaaggtgc aatccttcgt ccttcgtgtt tgtatgtcag
      781 agcatgccac ttgaacgggg gggggtagta acatgacgaa ggcagataag aagactcagt
      841 tgttcgccgt agtccttgtt ggcgctgagg aaggagatat agagaaccct ctggtgcgtg
      901 caacatcatc tgccgacgcc atctcagcag ctctgcacga cccaagatgg gaaggatacg
      961 catgcacctt tggctcttga cattggtctc gattgagcgc gatgacggcc tgggcagata
     1021 gatctaacct ccgatcctga gcaaagaaaa ggcccagatt ccattgggcc tgggcaatcc
     1081 gaaggagcct tcaaacgtat gtggcggttt gactcgtatt tgctgtatca ttaagtagaa
     1141 cgccccgcat gggcaattgc aacgcatagc cgctgaaatg cttacgaccg cctatctggc
     1201 agacacagga ggaatcggca catgagattt ctgatcgatc ctgaagtatt cgaggtattg
     1261 cccactatgt gcattggcgt tgttgtggcg cacggcatcg acaataccca actccaatca
     1321 gacaatgctc agaaaatcag tgctcttctc agtaaagcta tgcgccgaac tagggccaag
     1381 tttgcgggga caaatgtgaa ggagcatcct gacatcgtac cctaccgaga ggcatttcgg
     1441 gcgttgggat ataacccgaa caagttcccc tgctcggttg aggccctcac atcccgggta
     1501 gtcaaaggga gcgatcttcc cgacataaac tccgttgtga accttgtaaa cgccgtgtcg
     1561 ctcgagctcg cgttgccaat gggggcgcac gacctcgact ccatctccgg cgatctcgcg
     1621 gtgcgattct cgcgccaagg cgaactcttc accccgctcg gtgaagctga gcccgaagaa
     1681 gtcccggcag gagagttggt gtatgccgat ggacgggaaa taaggacaag gcgatggata
     1741 tggcggcaga acgaccgcgg aaaagtgaca gaggagtcaa ggaacatctt cttgcccata
     1801 gatgggttca cagaccgcaa tcgtgatgct gttgaacggg ctatggccga actcgccggc
     1861 cgactcgaag agttcttcgg tgtggcagcg caccaatatt tcctcgatcg agaccgtaac
     1921 tcgtgcgact tgatggcctc agtcgaacaa aaaaaggcct gattcgcaag gacaaagtgg
     1981 atctcgggct tctcaagaca ggctcgctat gggcctctgt tgtcagaaca cgaaagttga
     2041 gtcagcacat agccccgatg aagccaattg tctgaagttt tgccactcct tttcggaccg
     2101 ccttcaccaa aggagcagca aggtcataac taacctgaag gtaactcaat cgagtagggt
     2161 ggcgccaggt aatcagcctg g
//
LOCUS       DAOSAI010000396         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_33333_length_2166_cov_3.127901, whole genome shotgun sequence.
ACCESSION   DAOSAI010000396 DAOSAI010000000
VERSION     DAOSAI010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33333_length_2166_cov_3.127901"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLM83_10575"
     CDS             complement(<1..305)
                     /locus_tag="PLM83_10575"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOK71887.1"
                     /translation="MAYCPACGAGCNPDDKFCKECGARLKILDSSEPERHMTAQEEVL
                     WEGKPYLKEIARNTNYVVTNQRILITTGLLGRKEEQIDLARVKDIRMTQGLSDRIMG"
     gene            complement(370..513)
                     /locus_tag="PLM83_10580"
     CDS             complement(370..513)
                     /locus_tag="PLM83_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71888.1"
                     /translation="MKSLVERPMKRNLSELVEVGYVLMYLLVIEDIALPSGCLCCRLV
                     LAW"
     gene            complement(864..>2166)
                     /locus_tag="PLM83_10585"
     CDS             complement(864..>2166)
                     /locus_tag="PLM83_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009658587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOK71889.1"
                     /translation="YDSWKLTSPAQRSQILLEIADRIDANTEKLAMIETLDNGKPIRE
                     SATLDVPLGADHFRYFAGVARAEEDSAVMIDDQTLSIILREPLGVVGQIIPWNFPFLM
                     AAWKIAPALAAGDTIVIKPSSATPLSILEFAKIIQDVVPPGVVNVVTGRGSTTGQYML
                     EHPGFRKLAFTGSTEVGYNVARAAADKIIPATLELGGKSANIFFPDAPWEKAIEGVQI
                     GILFNQGQVCCAGSRVFVHEDIYDKFLAECVEKFNSIKVGLPWKKETVMGCQINDGQL
                     QKILKYIEIGKDEGAKVVCGGTRIEEEELARGPFMRPTILADVTNDMRVAQEEIFGPV
                     VCFIKFKDEDEVIKMANDSEYGLGGAVWTRDINRALRVARGVETGRMWINNYNNIPAH
                     APFGGYKKSGIGRETHRMMLDHYTQKKNILIHISEAKMGMY"
BASE COUNT          478 a          657 c          510 g          521 t
ORIGIN      
        1 atccccatga tcctatctga aagcccctgg gtcattctga tgtccttgac gcgggccaag
       61 tcgatctgct cttccttccg gccgagcaga cctgtggtga tgagaatcct ctggtttgtt
      121 accacgtaat tcgtgtttcg cgcgatctcc ttgagatatg gcttgccctc ccatagcacc
      181 tcctcttggg cagtcatgtg cctttcaggt tcagaagagt caaggatctt gaggcgtgca
      241 ccgcattcct tgcaaaactt atcatctggg ttgcagcctg caccacatgc aggacaatat
      301 gccataacaa acgcctccct tattgcaacg gcctttccca tagccgatca gcccaattga
      361 aaaagtgcat taccaagcaa gaactagtct acagcacagg catccactag gcaaggcaat
      421 gtcctctatc acaagcaggt acatcagaac gtaccctacc tccaccagtt cactcaggtt
      481 ccgcttcatg ggcctctcga caagagattt caccctcaaa cgtcccctat accatgccca
      541 tgcatggcca actatgatga catttacgga tgtcttgcct cacgacaaaa gcacatacag
      601 cccgatatcg tgccacagcg tctagcagac cttctcgaag tgggcaccgt ccaagcggag
      661 ggaacgcgga atcacatccc cgcttccgat ggaagacctc gatttcaaac ggaacctccc
      721 tttcgcgcaa cgctcagtcc atacaataat ccaggcgggt cagcgttcgg catctataag
      781 aaacggcgcg ccgtcccgcc ggggcataaa cccccgcgag tcggcgcgct tcaatgagcc
      841 actaggcccg atagttgcag aaactagtac attcccatct ttgcttcgga tatatggatg
      901 aggatattct tcttctgagt atagtggtcg agcatcatcc gatgcgtctc acgcccgatt
      961 ccggacttct tgtaaccacc gaatggggcg tgcgccggga tattgttgta gttgtttatc
     1021 cacatacgtc ccgtctcaac acctcttgca accctaaggg ccctgttgat atcgcgtgtc
     1081 cagaccgcgc ccccaaggcc gtactcgctg tcgttggcca tcttgatgac ctcgtcctca
     1141 tccttgaatt tgatgaagca gactaccggc ccgaagatct cttcctgcgc cactctcatg
     1201 tcattggtca catcagccaa gatcgtggga cgcatgaacg ggccccgggc aagctcttcc
     1261 tcttcaatgc gagtgccgcc acagacgacc ttggcgccct cgtcttttcc gatttcaatg
     1321 tacttcaaga tcttctggag ctgtccatcg ttgatctgac agcccatgac ggtctccttt
     1381 ttccagggca agccgacctt gatgctattg aacttctcaa cacactccgc caggaacttg
     1441 tcatagatat cttcgtgcac gaatactctc gaacccgcgc agcacacctg tccctgattg
     1501 aacaggattc cgatctgaac gccttcgatg gccttctccc agggggcatc cgggaagaat
     1561 atgttggccg actttccgcc cagctcaagt gtggctggaa tgattttatc cgctgccgcc
     1621 ctcgcgacat tgtacccaac ttctgtggaa ccagtgaaag cgagtttgcg gaaaccggga
     1681 tgctccagca tgtactggcc tgtagtggag ccccgccctg ttactacatt gacgactccg
     1741 ggcggaacaa catcctgtat gatcttggca aattcgagaa tactgagtgg cgtggctgat
     1801 gacggcttga tcactatggt gtctccggct gccaaggccg gcgcaatctt ccacgcagcc
     1861 atcaagaacg gaaagttcca cggtatgatc tgaccaacaa cccctagcgg ctcccgcagt
     1921 atgatgctga gagtctggtc atcaatcatg accgcgctat cctcttcagc tcttgctacc
     1981 cctgcaaagt agcggaagtg atccgccccc aacggaacgt cgagcgtcgc gctctcgcga
     2041 atcggcttgc cgttgtctaa cgtttcgatc atggccagtt tttccgtgtt tgcatcgatg
     2101 cggtctgcga tctcaagaag aatctgcgaa cgctgtgcgg ggctggtcag cttccatgaa
     2161 tcgtac
//
LOCUS       DAOSAI010000397         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_33921_length_2142_cov_3.145184, whole genome shotgun sequence.
ACCESSION   DAOSAI010000397 DAOSAI010000000
VERSION     DAOSAI010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33921_length_2142_cov_3.145184"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..575
                     /gene="rimI"
                     /locus_tag="PLM83_10590"
     CDS             75..575
                     /gene="rimI"
                     /locus_tag="PLM83_10590"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOK71890.1"
                     /translation="MDLIPSPMNLDDLDRVLDIERLSFSLPWSRHAFVDELLMNDQAV
                     YMVVRDSDGVAMGYGGMWLILDEAHITNVAVHPEYRRRGVGRAIMVALMETAVLRGAR
                     RMTLETRLSNVPAQELYRSLGFVAAGVRPRYYLDDHEDALVMWLEDLPGRLAMMDGEG
                     GAPEDA"
     gene            568..1605
                     /gene="tsaD"
                     /locus_tag="PLM83_10595"
     CDS             568..1605
                     /gene="tsaD"
                     /locus_tag="PLM83_10595"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOK71891.1"
                     /translation="MREDGVVLGIETSCDETAAAVVRGGREILSSVVASQIEVHRRYG
                     GVVPEIASRRHVEAIIPVIDEALAQAGTDLAEIDAVAATHGPGLVGALLVGLSAAKGI
                     SYAAGKPLICVNHIEGHIYANFLAHPQLSPPVLCLTVSGGHTELIAMRDHGRYEVLGR
                     TRDDAAGEAFDKVARVIGLPYPGGPALEQLAKSGDPNLISFPRAEVEGSPLDFSFSGL
                     KTAVINYIHTAEQRGEDLDYADVAAGFQKAAVDELVSRVDRAVDFTGMSVVAVSGGVA
                     ANQALRRALTALAGKSNIEVYYPPLNLCTDNAAMIASAGYYRHVAGEVSSLSANAEPG
                     ALLSSLACFDG"
     gene            1682..>2142
                     /locus_tag="PLM83_10600"
     CDS             1682..>2142
                     /locus_tag="PLM83_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOK71892.1"
                     /translation="MSEVGERNVRQNASAGRSNTGRIWATIVIVVVAACCGLFLYFTY
                     LPAENGLFNILILGEDRNYSRNGDVLSAPGRPDTILLLMVPKSSRGAALLSIPRDTLV
                     RFPNGGVRRINSSVPEGGVDLARELVSDLVGLPVHRHVVVDFGGFVELVDA"
BASE COUNT          435 a          493 c          693 g          521 t
ORIGIN      
        1 agcccatgtg cttcctatgt accttcgtcc gaccgaggcc gaggtaaagc tctcccggaa
       61 ggagatggaa aaggatggat ctgattccgt ctcccatgaa tcttgatgat cttgacagag
      121 ttctcgatat tgaacgtctg tcattctctc ttccatggag caggcatgcg ttcgtggatg
      181 agcttctcat gaatgaccaa gccgtgtata tggttgtgag ggactccgat ggagttgcaa
      241 tgggatacgg cgggatgtgg ctcatcctcg acgaggccca tatcacgaat gtcgcggttc
      301 accccgaata tagacggcgt ggggttggac gtgctatcat ggttgccttg atggaaaccg
      361 cggtcctccg gggcgctagg aggatgactt tggagactag attgtcgaac gtgcccgctc
      421 aggagcttta ccgaagtcta ggtttcgtgg cagctggcgt gaggccgcga tactacttgg
      481 atgaccatga ggatgccctg gtgatgtggc ttgaggattt gcccggacgc ttggcaatga
      541 tggatggcga gggaggtgcc ccggaggatg cgtgaggacg gggtcgttct cggtatcgag
      601 actagttgtg atgagacggc ggcggcggta gtgcgcgggg gccgcgagat tctctcatca
      661 gtggtcgcgt ctcaaataga ggttcaccgt agatatggcg gggttgtgcc tgagatcgct
      721 tcgcgtcgac acgtggaggc gatcattccg gttatagatg aggccctggc gcaggccgga
      781 actgatctgg cggagatcga tgcagttgcg gcaacacatg gtcccggtct tgtcggggcc
      841 ttgctagttg gcctttcggc cgccaaggga atatcgtatg ctgcagggaa accgctgatc
      901 tgtgtgaatc atattgaggg acacatatat gctaacttcc tcgcccatcc ccaactgagt
      961 cctccggtct tgtgccttac ggtgtcgggc ggacacacgg aactcattgc catgcgagat
     1021 cacgggcgct atgaagtgct gggacgcacg agggatgacg ccgcaggcga ggcatttgac
     1081 aaggttgcaa gagtaatagg ccttccgtat cctggcggac ccgcccttga gcagcttgct
     1141 aagtcgggcg atccgaatct gatttcgttt ccccgagcgg aggtagaagg gagtccgctc
     1201 gattttagct ttagcggatt gaagacggcg gtaatcaact acatccatac cgcagaacaa
     1261 agaggcgaag accttgacta tgctgatgtg gctgcgggtt ttcagaaggc cgctgtagat
     1321 gagctcgtct ccagggtgga cagggcagtg gatttcaccg ggatgagtgt tgtggcggtt
     1381 tcgggagggg ttgcagcgaa tcaggcattg cgaagggcat tgacggcgct ggctgggaag
     1441 agcaatatag aagtatatta tcctccactc aatctgtgca cagacaacgc ggccatgata
     1501 gcctctgcag ggtactacag acatgtggct ggagaggtgt catcgctgtc agcgaatgct
     1561 gaaccaggtg ccttgctgag cagcctcgcc tgctttgacg gctaggtttt tcaggaggat
     1621 acgaggcgtc cctgtagaaa tgaattaatc agtgcttcgg tgcgtctaag gagtaggtgt
     1681 catgtctgag gttggtgaaa gaaatgtgag acagaatgcc agtgctgggc gttcgaatac
     1741 gggccgaatc tgggcgacga tagtaattgt ggtggtagct gcgtgttgtg gtctttttct
     1801 atacttcaca tatttgcctg ccgagaacgg gctctttaac atcctcattt tgggggaaga
     1861 ccgtaactac tcccgtaatg gcgacgtctt gtctgcgccg ggcaggcccg acactatcct
     1921 gttgctcatg gtcccgaaga gttcccgggg tgcggcactt ctatcgattc ctagggatac
     1981 tctggtaaga ttccccaatg gcggggtaag gcggattaac tcttctgttc cggaaggggg
     2041 ggtggatctc gctcgtgagc tagtttcgga tctcgtgggg ctgccggtgc acagacatgt
     2101 cgttgtggac tttgggggat ttgtggaact cgtagacgcc tt
//
LOCUS       DAOSAI010000398         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_34224_length_2130_cov_3.643373, whole genome shotgun sequence.
ACCESSION   DAOSAI010000398 DAOSAI010000000
VERSION     DAOSAI010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34224_length_2130_cov_3.643373"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLM83_10605"
     CDS             <1..653
                     /locus_tag="PLM83_10605"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HOK71893.1"
                     /translation="RAEEGPITGKRIWDSLKYAFWVICHPFDGFWDLKHERRGNTVSA
                     TIILVLIALTHVFARQYTGFVLNKNDPKRLNVLLEISTVLVPFALWIVVNWSLTTLTD
                     GKGTLKDIYIATAYALFPIFLINVPLTIWSRYMTMEQGAFFTFFRVLSLIWAGWLVFS
                     GNMVTHDFEGGKTFGMSLLTVAGMGVVIFISLLYASLIDQFIGFVKDIRTEIVFRM"
     gene            691..>2130
                     /locus_tag="PLM83_10610"
     CDS             691..>2130
                     /locus_tag="PLM83_10610"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOK71894.1"
                     /translation="MRQRMILVAAAVGLLGILCVAVLAAEIPGSLASSRKIVEPKGFK
                     AAAENDSLALFVNGSTTEIAVLDKKTGALWYSNPPDQDERETLARGMARDRLHSQLVI
                     SYFTPGDQRQFMTSCADSVAYGQYHIIPIASGVRVEYVMGAQWQEKDRLPLIISKKRM
                     DEVILAHMESEKDKEFILSSYMLVELRKGGTGERMTLPGWDASTVFRDYTVRLLEDSA
                     SDARWEPSLDEATKWLIAQLVENRSDIEDRSCLKHEDVSQMVNNPTYILMGDTTDWDR
                     EDIASLLAAAGYTADDVVRDHMENHYDPPKPNPRTFSLVIEYVLDGENLIVQVPCSEL
                     EYPIDIVNDEGEKETYPVESISILEFFGAAGQGQDGYMFVPDGSGALVRLNNGKTRFS
                     AVDLTLYGMDQAIMPLDESSAPPQACFPVFGIVANGRAMMAIIESGDAMARIKADVSG
                     RANCYNSVSSSFTVIPKGTVSSSGSGRNIS"
BASE COUNT          522 a          489 c          615 g          504 t
ORIGIN      
        1 atcgcgcgga agaggggcct atcacaggaa aacggatatg ggattcgctg aagtacgctt
       61 tctgggtaat atgccatcca ttcgatggct tctgggattt gaaacacgag cgtcgcggga
      121 ataccgtgtc tgccaccatc attcttgttc tcattgcttt gacacatgtg tttgcaaggc
      181 agtatacagg cttcgtcctt aacaaaaatg atcccaagag gctgaacgtg ctgctcgaga
      241 tcagcactgt gctggtgccg ttcgcccttt ggattgttgt aaactggtcg ctcacaacac
      301 tgacagacgg aaaggggacg ctgaaagaca tatacatagc aacggcctac gcgctctttc
      361 cgatattcct gataaacgtt ccattaacca tctggagcag gtacatgacg atggaacagg
      421 gcgcgttttt cacgttcttc agggtgctgt cgttaatatg ggcgggttgg ctagtctttt
      481 caggcaacat ggttacacac gattttgaag gcggaaagac gtttgggatg tcgctcttga
      541 ccgtagctgg catgggagtg gttattttca tttccctgct ctatgccagc ctgatagatc
      601 agttcatcgg ttttgtgaag gatattcgca ctgagatagt cttcagaatg taggcgtttg
      661 gatattgggg cgaacggagg tcttgaatac atgaggcagc gaatgatcct tgtggcagcc
      721 gcagtcgggc tgcttggcat attatgcgtg gcggtcctgg ctgcggagat accagggagc
      781 ctagcaagtt ccagaaaaat cgtagaaccc aaaggcttta aagcagctgc agagaatgac
      841 tcacttgcgc tgtttgtcaa tgggagcaca actgagatcg ctgtgctgga caagaagacg
      901 ggcgcccttt ggtattcgaa tccgccggat caggatgaac gcgagacctt ggccagggga
      961 atggccaggg ataggctaca ttcacagctg gttatctcgt attttactcc aggagatcaa
     1021 cgtcaattca tgacgagttg cgccgattca gttgcttacg ggcagtacca cattatacct
     1081 attgctagtg gtgtgcgggt tgaatacgta atgggtgcgc agtggcagga aaaggacaga
     1141 ctccctctga tcatcagcaa gaaacggatg gatgaagtca ttttggcgca tatggagagc
     1201 gaaaaggaca aggagttcat cttatccagc tacatgctgg tagagctcag gaagggcggc
     1261 accggtgagc gcatgacttt gccaggatgg gatgcctcca cagtctttcg cgactatact
     1321 gtaagattgc ttgaggactc tgcgtcggac gccagatggg aaccgtctct cgatgaagcc
     1381 accaagtggc tcattgcgca gctcgtcgag aacaggtcgg acatagagga cagatcgtgc
     1441 ttaaagcacg aggatgtatc tcagatggtg aacaatccga cctacattct gatgggcgac
     1501 acaactgatt gggataggga agacatagca agcctacttg cggctgcagg ctacaccgcc
     1561 gacgatgtgg tgcgcgatca tatggaaaac cactatgatc ccccgaagcc gaatccgagg
     1621 accttctcgt tggtgattga gtatgtgctt gacggtgaga atctgatcgt gcaagtgccc
     1681 tgctctgaac tggaataccc catagacatc gtcaatgacg agggtgaaaa ggagacatac
     1741 ccagtagaat ccataagtat acttgagttc ttcggcgccg caggacaagg ccaagacggc
     1801 tacatgttcg tccccgacgg ttcaggggcg ctagttcggt tgaataacgg gaagacgcgt
     1861 ttttcagcag tcgacctcac cctttacggg atggatcagg ccataatgcc acttgatgag
     1921 agcagtgcgc cgcctcaggc gtgctttcca gtattcggca tcgtagcgaa cgggcgggct
     1981 atgatggcca tcatagagag tggagacgcc atggccagaa tcaaggcgga tgtgtcgggc
     2041 cgcgccaact gctacaatag tgtttcgtcc agtttcacgg tgattccgaa gggaactgtc
     2101 tcatcgtcgg gcagcgggag gaatatctcg
//
LOCUS       DAOSAI010000399         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_34227_length_2130_cov_3.288675, whole genome shotgun sequence.
ACCESSION   DAOSAI010000399 DAOSAI010000000
VERSION     DAOSAI010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34227_length_2130_cov_3.288675"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="PLM83_10615"
     CDS             <1..1788
                     /locus_tag="PLM83_10615"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HOK71895.1"
                     /translation="LIYGRPMPWTSYTTVRIDGSDWVFGGKTTRRAGRSGMYGEQVRP
                     PQIEDGKVVCSYMLGDILVTQELSFVKSPSTRYLDTARIHYKLENTGSVTRSVGLRLM
                     IDTMLGANDGAPLRVGEKTIVSDTMLSRREIPDYWQAFDSIVEPSVTAQGTMAGGELT
                     PPTQIAFSDWGSFADNLWDVNLVHGREFVRARESDLDSAVAMYWSPSLLAPGEVRHYV
                     TYYGLGGITLAPGKLALGMTAPSEVGLSADGTASFLAVAYVSNTGEGPAHDVTASLSL
                     PIGFTLADGHARTRTVGDLEPGETAQVAWQVIASWGAVGGGNLEVNVSATNANPNKVS
                     RRVEILAPPRLLARISHLEALQVRGEEIYPWPVRLTVRVENTGGSPAHWARVKLDSIG
                     GFSVVGGDVTQLLLGTLEPNRPLDFTWVLGGHPDAGTHTILVSASADDVPASRASASI
                     QVPLLVPKVWVKPSKTRVMPGDIFSVDVMITNVPDLKSAKFDLRYDNDFVDLALVSRG
                     GLFAAGGELMPWSEGDSRSTPGRVAGTWGSLNSPGYAWGSAATFYFQARAMGDARFSV
                     ENLVLSDASGRVISLESVPTPCTVQITRQ"
     gene            1816..>2130
                     /locus_tag="PLM83_10620"
     CDS             1816..>2130
                     /locus_tag="PLM83_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOK71896.1"
                     /translation="MSEISKRLSITAISVLIVISLMSSAVLAAPVTIKDVPEDHWAYE
                     AVKLLVEKGYMGVYESGNFCGDDPVSRYLLAFVTAKLLRDVEAGRTTLSEDDMRVVRE
                     TAT"
BASE COUNT          454 a          524 c          666 g          486 t
ORIGIN      
        1 cttatttacg gacgccctat gccgtggact tcctatacga cggttcgcat cgacggttca
       61 gattgggtct tcggcgggaa aacgactcgt cgcgctggaa gatcaggtat gtatggcgaa
      121 caggtacggc ctcctcagat agaggacggc aaggtcgtct gcagctacat gctgggagat
      181 attcttgtta cccaggaact ttcatttgta aagagtccga gcacgcgtta cctcgatacc
      241 gcgaggatcc actacaagct ggagaataca gggagcgtga cgcggagtgt gggcttgcgg
      301 ttgatgatcg acacgatgct cggagccaac gacggagcgc cactccgtgt cggtgaaaag
      361 acgatcgttt cagatactat gctttcaaga agagagatac ctgattattg gcaagccttt
      421 gattccattg tcgagccttc tgttactgcg cagggaacaa tggctggggg cgagctcacg
      481 cctccaactc agatagcttt ctccgattgg ggttcgtttg ccgacaattt atgggacgtc
      541 aatctcgttc acggcaggga atttgtgcgc gccagagagt ctgacctcga ttctgcggtc
      601 gcgatgtact ggtcgccatc gcttctagca cccggggaag tgaggcatta tgtgacatat
      661 tatggcttgg gcgggattac cctggcccct ggcaagctag ctctgggaat gacagcaccg
      721 tccgaagtag ggctttctgc tgacgggacg gcctcgttcc tcgcagtggc ctacgtaagc
      781 aataccgggg aagggccggc tcatgatgtg actgcttctc tgtcgcttcc aatcgggttt
      841 accctggcag atggacatgc caggacccga acggttggcg atcttgagcc gggagagacc
      901 gcacaggtag catggcaagt gattgcctca tggggtgctg ttggcggcgg caacctagag
      961 gtcaatgtga gcgcgaccaa cgccaacccg aacaaggttt caaggcgtgt cgagatactg
     1021 gcgcctccgc gcctgttggc gaggataagc catttggaag ccctccaagt gcgcggggag
     1081 gagatctacc cgtggcccgt gaggcttacg gttcgtgtgg agaacaccgg aggatcgccg
     1141 gctcattggg ccagggtcaa gctggactcg attgggggct tctccgttgt cggaggcgat
     1201 gtaacgcagt tgctgcttgg gacgttggaa cccaatcgcc cactcgactt cacttgggtt
     1261 ctggggggcc atcccgatgc aggcacccac accattctgg tgagcgcttc tgctgatgat
     1321 gtgccggcgt ctagggctag cgcatctatt caggtgcctt tgctcgtgcc aaaggtttgg
     1381 gtgaagccca gcaaaacacg ggtaatgcct ggcgacatat tcagtgtgga cgttatgata
     1441 accaatgtcc ctgatctgaa atctgctaag ttcgatttga ggtacgataa tgacttcgtc
     1501 gatctggccc tcgtgtcaag aggcggacta tttgctgcag gcggtgagct catgccgtgg
     1561 agcgagggag actcccgatc gacgccgggc cgagttgcag ggacgtgggg ctccttgaat
     1621 tcgcctgggt atgcttgggg tagcgcggca acattctact tccaggcgag agcaatggga
     1681 gatgcgcgat tttccgtaga aaatctcgtt ctttcggacg cgtcaggcag ggtgatatcg
     1741 ctggaatccg tccctactcc ctgcacggtt cagatcacca ggcaataaca tcaacggtca
     1801 aatggggggg tcatgatgtc tgagatttcg aagcggctga gcatcacagc gataagtgtg
     1861 ctgatagtga tctcgctaat gtcctcagca gtcctggcgg cgccggtcac aataaaggac
     1921 gtgccagagg atcattgggc atacgaagca gtgaagcttc ttgtagaaaa gggatatatg
     1981 ggcgtatatg agagcggcaa tttctgtgga gatgatccgg tcagcaggta tttgcttgcc
     2041 tttgtgacgg caaaactcct ccgtgatgtg gaggcgggcc gtacaactct gagcgaggac
     2101 gacatgagag tggttaggga gacagccacg
//
LOCUS       DAOSAI010000400         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_34498_length_2118_cov_3.584101, whole genome shotgun sequence.
ACCESSION   DAOSAI010000400 DAOSAI010000000
VERSION     DAOSAI010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34498_length_2118_cov_3.584101"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLM83_10625"
     CDS             <1..649
                     /locus_tag="PLM83_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOK71897.1"
                     /translation="EEAHDYRYFPEPDLGPIVIDRAWVEKIRVELPELPDAKAERYMR
                     EWDLPKYDACQIADSADLSDLFEKTCAIVGEGREVAKWILGEVMASLNATGIELSESK
                     LTPELFAELIDLMTTGKISGKIAKSVFEDVFSNGRRPSDIVKERGLLQISDVGELVTI
                     VEKVIDANPGPVADYHAGKERALGFLVGQVMKETRGRANPQETNRILREELDKRA"
     gene            complement(1190..>2118)
                     /locus_tag="PLM83_10630"
     CDS             complement(1190..>2118)
                     /locus_tag="PLM83_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK71898.1"
                     /translation="PEMYPSLTQRSQVSALRQALGIVGMIIGMVLAPQFRQWFGWAGM
                     GILFAVIGGLSMFLSLLGSKENPDFSEQQGLRVSEAFKHTFVNKSFVTFVLASLLIQF
                     TFVLLPATMPFFCKYVLGLTDAQNTLVLAPIFLVALPMVWVWGRVTNRIGPRRTAMVA
                     AVVFAVALIPFGYVTTLLGGMITSAFAGIGLAGLMVIIDVLIADVIDEDELGTGTRRE
                     GAYFGANAFVMRLGVSLEAITFSAIMGMHGYNPNLASQPSTVIPGFRILMTVVPIVSL
                     VLALIALYYYPIDGDRREAIIQGIEKKRSAEG"
BASE COUNT          528 a          488 c          622 g          480 t
ORIGIN      
        1 ggaagaggcg catgactatc gctatttccc cgaacccgac cttggtccaa tcgtgattga
       61 tagggcatgg gtggagaaga tccgagttga gctgcctgag cttcctgacg ccaaggctga
      121 gagatacatg cgcgaatggg atttgccgaa gtatgatgct tgccagatcg cagactccgc
      181 agacctttcg gatttattcg agaagacctg tgccatcgtg ggagaggggc gagaggttgc
      241 caagtggata ttgggagagg tcatggcgtc tcttaatgct acaggtattg agctgtcgga
      301 atccaaactc accccggagc ttttcgcaga gctcatagat ctgatgacga ctgggaagat
      361 atccggcaag attgccaagt cagtctttga ggatgttttt agtaacggca ggcgcccaag
      421 cgacatagtg aaagagcgcg gattgctaca gataagcgat gttggtgaac ttgtgaccat
      481 tgtcgaaaag gtgatcgacg ctaatcctgg ccccgtagct gactatcatg ccggcaaaga
      541 gagggcgttg gggttcttgg taggtcaggt catgaaggag actcgtggcc gggcaaatcc
      601 tcaggagact aacaggatcc tcagggaaga gttggacaag agggcttgag gacatattgt
      661 taagctcgaa gaatttgggt tgcccctcgt gcaagagcat ggatctagat agtatttgtt
      721 tgagtgcaaa gcaagggcgt gcagacgagt attcgtctgc gcgcctcatc gtgcgcctgg
      781 ggcatgttcg gtgctaataa gatgaaagtc ccgaacgagg gtaatgacaa gggtgttgtt
      841 gcgaggcaaa gcccgaggac tggctaacct gggatgaact ggccccacct gaaatcgagt
      901 ttgagtttgc cagtatgata atcctttcag atgcagtgtc cgggacttgg acgcgcattt
      961 caggtcttag gcaaaacgta accagttaag acttagatta gtcaaccagg gccgggcctt
     1021 taagcgtggg gtggttgact gtcgtgcgga tgaggttgaa cgcacaatat ggagcttagg
     1081 acttccagat ttgggtagtg aaagaacccc gatagatcca tggaaccaac ctccatctgg
     1141 gccatgccga tgctaaagtt tactgtgaaa gctgtgagag atgttcacat tacccttccg
     1201 cactgcgctt cttctctatt ccctgtatga tggcttctct acggtctccg tcgatggggt
     1261 agtaatagag tgctatcagt gctagcacta gcgagactat ggggactact gtcatgagaa
     1321 tcctgaaacc tggaatgact gtgctaggct gagaggcgag attcgggttg tacccgtgca
     1381 ttcccatgat cgcactgaag gtgatagcct cgagggagac tccaaggcgc ataacaaatg
     1441 cgttcgcgcc aaagtaggcg ccttctcgac gggttccagt accgagttcg tcttcgtcaa
     1501 tgacgtcggc tatgagaacg tcaataatca ccatgaggcc agcgaggcct atgcctgcga
     1561 aggccgaagt gatcatcccg ccaaggaggg ttgtgacgta tccgaaagga ataagggcta
     1621 ccgcaaacac gacggctgct accattgctg tgcgtcgcgg ccctatgcga ttcgtcacac
     1681 gtccccacac ccaaaccata ggaagcgcga ctagaaatat gggcgccaac acgagcgtat
     1741 tttgagcgtc ggtgagtccg agaacatact tgcagaaaaa cggcatggtg gctggcaaca
     1801 gaacgaaggt gaactggatt aatagacttg cgagaacgaa cgttacaaag gacttgttca
     1861 cgaaggtgtg tttgaaggcc tcacttacgc gtagtccttg ctgctcgctg aagtctggat
     1921 tctctttcga cccgagcagg cttaggaaca tgctcaatcc gcctatgacc gcgaagagaa
     1981 tacccattcc ggcccagccg aaccattgcc tgaactgagg agcaaggacc atgcctatga
     2041 tcattcctac aattccgagg gcctggcgaa gggcggatac ttgggaacgc tgtgtcagcg
     2101 agggatacat ttcggggt
//
LOCUS       DAOSAI010000401         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_34691_length_2110_cov_2.688078, whole genome shotgun sequence.
ACCESSION   DAOSAI010000401 DAOSAI010000000
VERSION     DAOSAI010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34691_length_2110_cov_2.688078"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PLM83_10635"
     CDS             complement(<1..1441)
                     /locus_tag="PLM83_10635"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOK71899.1"
                     /translation="MAPTIVSLKDVTFAYKGSKRQAIKGVSLDVKEGECVAILGPTGA
                     GKSTLACVINGTVPRFLEGELTGEVVVDGQVPNEVGTAQMASKVGLVFGDPDTQLFGM
                     TVEEDVAFGPANLGFDYDTIMGRVNRAIEDMRLKGLEQRAPHRLSGGQKQATAIAGVY
                     AMLPKIMVLDEPTSMLDPEGKARVFSIVRHLNKSLGMTIILIEQEVDDILQLADRVYV
                     MKSGKFVFGGTPREVFGSVNDLKSAGVRVPHIVEFGDLIGSAEAPFSVEEAISVVERG
                     AWQPAASVETGLADDQPISGSSGNKMQHGALTTSFDGVSPAVEVIDLEFKYPMGAKAL
                     NGVNLKVDSGEFVAIVGQNGAGKTTLTRHLNGLLRPSSGDIRLFGESILNESTTKLCP
                     MVGYVFQNPDEQLFCTTVEDELRFGPSNIGMDKDRMEQAVRDILLDIGLDKYRQVWPR
                     YLTKGERQRLALASVVAMEPRILIVDEPTT"
     gene            complement(1454..>2110)
                     /locus_tag="PLM83_10640"
     CDS             complement(1454..>2110)
                     /locus_tag="PLM83_10640"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOK71900.1"
                     /translation="IQMPLGQGAVGLGVSYALAICYLLLMKLAGDILTKQYPEEHKVV
                     WDTRTISRVTILAAVSGAGAMIKIPGPATTIALDAAAGYFAASYFGWPIGATVAAIGC
                     FLANALGGFASWLPMVPIYVCAMAFTVTIGVFVAKKTNIVLAIIVGTVVNTITCLGPW
                     VVMIGPALMVALLPSQLIGSAVNAAVGLTVAQALKSAQTHEKSDVEELVEPEDESFKM
                     "
BASE COUNT          489 a          649 c          479 g          493 t
ORIGIN      
        1 cagtagtcgg ctcatcgacg atgagtatac gaggttccat agccacgaca gacgccaggg
       61 caagtctctg cctttctccc ttggtgaggt aacgcggcca tacttgcctg tacttgtcga
      121 gacctatgtc gagaagaatg tcgcgcactg cctgctccat tctgtcctta tccatgccga
      181 tatttgacgg cccaaatctc agttcatcct cgacggtggt acaaaaaagc tgctcgtccg
      241 ggttctggaa tacgtagcca accataggac atagcttcgt tgtcgattca ttcaagattg
      301 actcgccgaa aaggcgtatg tctcctgatg aaggcctaag cagtccgttc agatgcctcg
      361 ttagagtggt cttccccgct ccattctgcc cgacgatggc cacgaattct cccgaatcca
      421 ccttcaggtt tacgccattt aacgccttag ctcccatcgg atacttgaac tcaagatcga
      481 tcacttccac tgcaggcgac acaccatcaa atgacgtcgt caacgcgcca tgctgcattt
      541 tatttccact gctcccagag atgggctgat catctgccag gccagtctcc acacttgcag
      601 ccggttgcca ggctccgcgc tctaccacag agatggcttc ttccactgaa aaaggcgcct
      661 cggcagatcc tatcaagtct ccaaactcga ctatgtgagg taccctcacg ccagctgact
      721 tgagatcgtt aacactgccg aagacctccc gcggcgtacc cccaaaaaca aacttgcccg
      781 acttcatgac gtacacccgg tccgccagct gaagtatgtc atctacctct tgctctatta
      841 gaatgatggt catcccgagc gacttgttga ggtgccgcac tatcgagaag acgcgagcct
      901 tcccctccgg atccagcatg gatgtgggtt cgtcgagcac cattatcttg ggcaacattg
      961 catagactcc cgcaatcgct gtcgcctgct tctgcccgcc ggagagacga tgcggggccc
     1021 tttgctccaa acccttgagc ctcatatcct caatggctct gtttactcgg cccatgatcg
     1081 tgtcataatc gaacccaagg ttagccggcc caaacgccac gtcttcttct actgtcattc
     1141 caaaaagctg cgtatccgga tctccaaaaa ccaatcctac cttggacgcc atctgcgcag
     1201 tgcctacctc gttaggcacc tggccgtcaa cgacgacttc cccggtaagc tccccctcga
     1261 gaaaccgcgg gactgttccg ttgatgacac acgcaagagt gctcttgccc gcaccggtag
     1321 gccccagtat tgctacgcac tcgccctctt tgacatcaag cgaaacacct tttatagctt
     1381 gtcttttcga tcctttgtag gcaaaagtca cgtccttaag cgagacaatg gtgggcgcca
     1441 actgcaacac ctcttacatc ttgaaacttt catcctcagg ctctacgagc tcttcaacat
     1501 cgctcttttc gtgcgtctga gcactcttca gcgcctgggc tacggtcaaa ccaacggccg
     1561 cattcacggc cgatccgatc agctgagacg gaagaagagc aaccatcaga gctgggccaa
     1621 tcatgactac ccatgggcct agacaggtga tcgtgtttac cactgtgccg acgattatcg
     1681 ccaacacaat attggttttt ttggctacga aaactccgat cgtgaccgtg aaagccatcg
     1741 cacacacgta tatcgggacc atcggcaacc agctggcaaa gccccccagg gcatttgcta
     1801 ggaaacaccc tatggcagcg acagtggcac ctataggcca tccaaaatac gaagctgcaa
     1861 aatagccagc agcagcatca agagcaatcg tggtagcagg tccaggtatc ttgatcatcg
     1921 ctcctgcccc tgatactgcg gccagtattg tgactcgaga gattgtgcgc gtatcccaaa
     1981 ccaccttgtg ttcctccgga tattgcttgg tcagtatgtc tccggctagc ttcatcagaa
     2041 gcaagtagca gattgccaat gcgtatgaga ctccgagacc cacagctcct tgtccaagcg
     2101 gcatttggat
//
LOCUS       DAOSAI010000402         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_34935_length_2099_cov_4.193738, whole genome shotgun sequence.
ACCESSION   DAOSAI010000402 DAOSAI010000000
VERSION     DAOSAI010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34935_length_2099_cov_4.193738"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2099)
                     /locus_tag="PLM83_10645"
     CDS             complement(<1..>2099)
                     /locus_tag="PLM83_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOK71901.1"
                     /translation="DEFQVPATVSRKRLMIESSLVLDCLEVARLLGAIGRPFDIMPLV
                     ASPYLGLGPRRAGQIARAINLKGLELPLAGWEAKLEKCDPSGDCDGQRYAAPLAEAAE
                     QAEDVRRASGSSGLAGYVDGFRAALARLGMPDRIFALPGDPMLGAEEWMAWATFHSLL
                     LEISNARDVWDASREPTASSMAGWTDFYSLLSFAAGRRSFALNRPSPNGVEVMGLDRA
                     ASCDREFLFVSGLGEQMLPRRRRRPWMLQESDVQALARAGVSLEPMGNGAEADRHVFN
                     TLVHSRAERIYLSYATAKEDGSLARRSFFVDEHLRSLGVSDADEHEITQTVPASSVFP
                     MRWDDVASKREARMRSLWSMGRVNAAGMGSMPAEPKPEAGACLWLRNRDVAFAKLAEV
                     WLRRQVAEIAEEDGYGHFRGCIGCDELLEKMAATVFDVRPWSANSLNEYIKCPFSFFC
                     QSVLGIEPPDEVGDDVDARDKGTCLHEIVKEFISRHIGEPLDPNKADQYCEEITRIAS
                     DVVDRTPSERSGVPASVWDAYFAGIIAASRDFVIKELEFTEATGGVWRPIHLEWPFGS
                     SVRQLAVGEGRVPVTGRIDRIDAGPGGALAIYDYKSGKTVPSARNIKEVADIQLGLYA
                     LAAESLLKAEVAGVWYYQVPSLGRTEGVWRAGYHQHFNAGRKRAGKLDDDQWCAFAEL
                     VEETVERCDRGARQGMF"
BASE COUNT          464 a          715 c          509 g          411 t
ORIGIN      
        1 aaaacatgcc ctgccgcgca cccctgtcgc acctttcaac agtctcttcc accagttccg
       61 cgaaggcgca ccactggtca tcgtcgagct tgccggcgcg cttccgtcca gcattgaagt
      121 gctggtggta acctgccctc caaacgcctt cagttctccc taaggaggga acttggtagt
      181 accacacccc tgcaacctcc gctttcagca gactctcggc tgccaatgca taaagcccga
      241 gctgaatgtc ggcaacctcc ttgatattcc tcgcactggg cactgtcttc cctgatttgt
      301 aatcgtatat ggcaagagcg ccgcctggtc ccgcatctat cctgtcgatc cggcctgtaa
      361 cgggcacccg accctccccc acagccaact gccggacaga tgacccgaat ggccattcaa
      421 gatgtattgg gcgccatacc cctcccgtag cctcagtaaa ctccaactcc ttgattacaa
      481 agtcgcgaga cgccgcgatg atcccagcaa agtaggcatc ccagacagac gcggggaccc
      541 cggagcgttc ggaaggagtg cggtccacca cgtcagaggc tatcctagta atctcttcac
      601 agtactgatc ggccttgttt ggatccagcg gctcgcctat gtggcgactg ataaattcct
      661 tgacgatctc gtgcagacat gtgcctttgt cccttgcgtc cacatcgtcg ccgacctcat
      721 ccggcggctc gattcctagc acgctttggc agaagaaact gaacgggcac tttatgtatt
      781 cgttgagcga gtttgcagac cagggccgca cgtcaaaaac agtggcggcc attttttcga
      841 gaagctcatc acatccaatg cacccgcgga aatggccata cccatcctcc tccgctattt
      901 cagcaacctg ccgcctgagc cacacttcag caagcttggc aaaggccacg tcgcggttcc
      961 gcaaccataa acatgccccg gcttcgggct tgggctcagc aggcatcgac cccatcccgg
     1021 cggcattcac ccgccccata ctccaaagag acctcatcct cgcctcccgc ttgctcgcca
     1081 catcgtccca tcgcatggga aagaccgaac tggcaggcac cgtctgggtt atttcgtgct
     1141 catcggcatc tgaaacgccg aggcttctca ggtgctcgtc tacaaagaac gagcgtcggg
     1201 ccagcgaacc atcctccttg gccgtagcat agctcaagta gatcctctcc gctcgcgagt
     1261 gcaccagcgt gttgaataca tgcctgtcgg cctctgctcc gttgcccatc ggctcaaggg
     1321 acacgcccgc ccttgccaaa gcctgaacat cgctctcctg aagcatccac ggcctgcgcc
     1381 tgcgccgagg cagcatctgc tcacccaaac ctgatacaaa caaaaactcc cgatcacacg
     1441 aagccgcgcg gtccaggccc atcacctcta ccccattagg cgaaggcctg ttgagggcga
     1501 acgatcttct gcctgcagcg aaagaaagga gggagtagaa atcggtccaa ccggccatag
     1561 aactcgcagt cggttcacgt gatgcatccc aaacatcccg agcattggat atctccaaaa
     1621 gcaacgaatg gaacgtagcc catgccatcc attcttcagc cccaagcatt ggatcaccag
     1681 gcaacgcaaa gatccggtcc ggcatgccca aacgcgcaag ggcagcccta aagccatcaa
     1741 cataaccggc cagtcccgag cttccgctcg cccttctcac atcttcagct tgctctgcag
     1801 cctccgccaa aggcgccgcg taccgctgcc catcgcaatc tcccgaagga tcacattttt
     1861 cgagctttgc ctcccagcct gcaaggggca gttccaatcc tttgaggtta atagcgcggg
     1921 caatctgccc ggccctgcgc ggaccgagcc ctaggtaggg gctggccaca agcggcataa
     1981 tatcgaacgg gcgcccgatc gcaccgagca gccgggcgac ttcaagacag tccagtacca
     2041 ggctggactc gatcatcaaa cgtttgcgcg agacagtggc cggcacctgg aattcatcg
//
LOCUS       DAOSAI010000403         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_35243_length_2086_cov_3.810438, whole genome shotgun sequence.
ACCESSION   DAOSAI010000403 DAOSAI010000000
VERSION     DAOSAI010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35243_length_2086_cov_3.810438"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PLM83_10650"
     CDS             complement(<1..1118)
                     /locus_tag="PLM83_10650"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HOK71902.1"
                     /translation="MRRASLLLMIAVALSAVCAFGAFAEESDALSIHPVNRLIQADPG
                     SVVTIVVAIENHSAEAVDVDLSSELPQSWFTFFGDETVRLAPGEIEILPIAVMIPKSA
                     PAGSYEVFVHARYTCPRMNDRQSSEIRFTVLVNEVRSIRVTLVNAPDYVVDTAYTASF
                     LLSNHGNVVEDLKLSTCDIQGAYIKLDPAWVSLEPGGSTPIDVHVILTAAPGRVLDHT
                     VLVKAGNRDLLSNWASASARVKVLPQKLSDSDAYHHFPLKIQLSAGVLGTSNRAQLKV
                     AGAGPLAHGGNDRLRFDLSDTGGVVEYSAPGMRLILGDGRFGLSPLTEWQAKARGIGV
                     QISQERAGLELYTHDGAGSPRVGLRAAATSSNGSSLSL"
     gene            complement(1124..1984)
                     /locus_tag="PLM83_10655"
     CDS             complement(1124..1984)
                     /locus_tag="PLM83_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71903.1"
                     /translation="MIYPNRRCVAGATTLALAVLLCVMMPPSAHASVNVLSGLTQMHE
                     VALGGEYSGVIVLENLGDVVERVELYQTDYRFTAEGYTYYEEPGTVRRSNAAWIRLGQ
                     SVVEIPPKCRISIPYRILIPNDTGLVGTYWSMVMAEMLGSVDEVDIDEPYKKDENAIV
                     LGVKQKFRYGIQIVTNIGVTGKKKVVFSNPALRRIESSGALTLSVDLANAGERLVNPS
                     VWVEVYDKTGTKIGRLEGKNAKIYPDTSVRATVELGALEPGSYRVIIVADNHDEYVAA
                     VRYSLEVSVP"
BASE COUNT          477 a          631 c          512 g          466 t
ORIGIN      
        1 tgaagggaaa gcgaagaccc gttgctagaa gtcgcggcag ccctaagccc caccctagga
       61 gagccagcgc catcgtgagt atagagctcg agtcccgccc tttcctgact gatttgcacg
      121 cctatgcctc gcgccttggc ctgccattcg gtcagcgggg acaggccgaa cctgccgtca
      181 cctaatatca gacgcattcc aggagcactg tattcgacaa cccctcccgt atctgataga
      241 tcaaagcgta gtctgtcatt gcccccgtga gcaagcgggc ctgccccggc cactttgagc
      301 tgcgcccgat tagatgtccc aagcacacct gcgctcagct ggatcttgag ggggaaatgg
      361 tggtaggcat cggaatcgct cagcttctgc ggaagcacct tcacccgagc gctggccgac
      421 gcccagttcg acagcaaatc ccggtttccc gctttgacaa gtactgtatg atcgaggacc
      481 cttccaggag cggcagtcaa gattacatgc acatctatcg gagtgctccc gcccggctcc
      541 aagctgaccc aggcagggtc gagtttgatg tacgccccct gaatatcgca ggtcgagagc
      601 ttcaagtcct ccacgacatt gccatggttg ctcaggagaa atgatgctgt gtaagcagtg
      661 tccacaacgt agtctggcgc attgacaagg gtaactctga tactcctgac ttcgtttacc
      721 aacacagtga accggatttc tgaggattga cgatcgttca tgcgcggaca tgtgtacctc
      781 gcatggacga agacttcgta acttccggca ggcgcactct tgggaatcat taccgctatg
      841 ggcaaaattt caatttcgcc cggcgccagc ctcaccgttt cgtctccaaa aaacgtaaac
      901 cacgactgag gcagttccga actgaggtca acatctaccg cctccgcact gtggttttca
      961 atcgccacca caatggtaac aacactgccc ggatctgcct gaatcaatct gttgacagga
     1021 tgaatcgaca acgcgtccga ctcttcagcg aatgccccaa aagcgcagac ggcggagagt
     1081 gcaacagcaa tcatcagaag taggctggct ctcctcacac cagtcaaggt accgacacct
     1141 ccaggctgta ccgaacagcc gccacgtact cgtcatggtt gtcggccact ataatcaccc
     1201 tgtagcttcc gggctcgagc gcgccgagct cgaccgttgc ccgaacggac gtgtcagggt
     1261 atatcttggc attcttgccc tcaagccgtc ctatcttcgt tccggtcttg tcgtatacct
     1321 caacccatac agacgggttg accaaccgtt cgccagcgtt ggccaggtct acgctcaatg
     1381 taagggcgcc ggaagattcg atgcgtctga gtgccgggtt gctgaagact acttttttct
     1441 tgcccgtaac gccgatattg gttactatct ggatgccgta tctaaacttc tgtttcactc
     1501 ctaggacaat cgcattctcg tccttcttgt aaggctcgtc aatgtcgacc tcgtctacac
     1561 tgcccagcat ctctgccatc accatactcc agtaagtgcc gacgagtccg gtgtcgttgg
     1621 gtatcaggat tctgtagggg atgctaattc tgcatttggg cggaatctct acaacagact
     1681 gtcccaggcg aatccaagct gcattggagc gtcgaacagt gccaggctct tcataatatg
     1741 tgtagccttc tgcagtaaac cggtaatctg tttgataaag ctccaccctc tcgaccacat
     1801 cgcccagatt ttccagtact atgaccccgc tatactcccc gcctagagca acctcatgca
     1861 tttgagtgag gccactcagc acgtttaccg aggcatgggc gctaggtggc atcatcacac
     1921 aaagcagaac agccagagcc aaagtagtgg cacctgcaac acatctgcga tttggatata
     1981 tcatgacaac atcgctccct tcgcttcaac cgtgcacaga gacatcctcc ctcttcagcc
     2041 agtacccgca cagggctgcc gatgggaaag gccgaggaag aacccc
//
LOCUS       DAOSAI010000404         2057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_35992_length_2057_cov_3.643357, whole genome shotgun sequence.
ACCESSION   DAOSAI010000404 DAOSAI010000000
VERSION     DAOSAI010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35992_length_2057_cov_3.643357"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLM83_10660"
     CDS             <1..311
                     /locus_tag="PLM83_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71904.1"
                     /translation="RRLPPAAQQTPRGLPAQFLTDCDTRELRHDAQFEVKVHDGVYGV
                     GACEFIGTLYEAIQLFQVSNSTFATGLIQIVGIGDKAGASQASSSRSKDRLWPLCLDV
                     "
     gene            859..1395
                     /locus_tag="PLM83_10665"
     CDS             859..1395
                     /locus_tag="PLM83_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOK71905.1"
                     /translation="MELWDVYDSNREKVGKTAVRGQGLEAGEYHVVVHIWLVNSEGNF
                     LIQKRAASVEAAPNIWATTGGSALAGENSAAACARELYEEIGVRADMGKARLAFTEKR
                     ADCFCDIWIIRQSINIEDCKLQVEEVSEVKWASPGEIRFMIAQGTFWGYRYIEGLFSL
                     VQADESESRPRKRSGFNS"
BASE COUNT          473 a          513 c          612 g          459 t
ORIGIN      
        1 ggcgtcgatt gccgcccgca gctcaacaga ctccgcgagg cctgcctgct cagtttctta
       61 cggactgcga tacccgcgag cttcgacatg atgcccagtt cgaagtgaag gtccatgatg
      121 gtgtgtatgg tgtgggtgcc tgtgaattca tcgggaccct gtacgaggcg atccagctct
      181 ttcaggtgag caattctacg ttcgccaccg gccttataca gattgtggga atcggggata
      241 aggcgggagc atcacaagcc agttcatcac gtagtaagga tcgactgtgg ccgctgtgcc
      301 tcgacgtcta gctggatcaa ccatcttctc aaggatgatt gtgaagggca gggtttgtat
      361 tcttagacag gaaatcggca ggtcatggtt tcaaccccat caagcacgaa gcatactact
      421 acattgtgga cctcactcac tacctactcg agcttgggca gaaaaacacg ccggagtctg
      481 tcgattggga ggattacatt cgcagcgaca ttctgctttg aaatgtgtac gaggtggttt
      541 cgcttgaaga gtacatatac cacttccggc tggaaaggga tttgcgatat gctattgcct
      601 tgtctgtagg cttcacggct ccggacgcgt ctcccgttgc ccacaagtat ccgggtgggc
      661 gtcttgaagt aacattccct gaagggttgg agctgagggt attgtatggt gatcctgatg
      721 acttcgcttt tgcccccgag ccggcaaaaa cgactaggag gaactttcgt cagcaggctt
      781 tgcaatgccg cagccctgac gtggggggcc aattgtggta gagtagttgc agtctgtttt
      841 gagaggcggg gcgcatttgt ggaattatgg gatgtatatg actccaacag agagaaggtt
      901 ggcaagactg cagtgcgagg gcagggactt gaggccggag agtatcatgt tgttgtccac
      961 atatggttgg tgaatagtga aggcaacttc ctgatccaga agcgtgcagc atctgtcgag
     1021 gcggcgccta acatctgggc cacgaccggc ggatcggcgc ttgccgggga aaacagcgcc
     1081 gctgcgtgcg caagagagct gtatgaggag atcggagtca gggccgacat gggtaaggcc
     1141 cggcttgcct tcacagagaa gagggcagac tgtttctgtg acatttggat catccgacaa
     1201 agcatcaaca tagaagattg caagctgcag gttgaggaag tgagcgaggt gaaatgggcc
     1261 tctcccggag agatccggtt catgatagcc caggggacgt tctgggggta ccgatacatt
     1321 gaaggcctgt tcagcctggt gcaggctgac gaatccgaaa gtcgcccaag aaagcgttcc
     1381 ggcttcaaca gctaatacag gcgtcggccc catctttgcc gctcccttca tggaatgctg
     1441 acacatactt ggatcatctt ggcgcttggg gcccgttaaa agcccagcat agcccgccaa
     1501 tctggaacag atcgactcag caggtcgccg tgcggtggga aaacgaagag gaaggcgctg
     1561 cggtgatagt cggaatactc tgaggctggg aaagccagcc acattgggaa gcggcgaggc
     1621 tccacaaagg ctaaaacgcg aggcctcttt ccacctgctt cattctgcac tgcatacata
     1681 aaaaagtaga cgatgcgggg acagcattcc tcatgtccgg cagttcagtg ttgccaatca
     1741 aggctgcggt cgtcgtaagc gctagagtct tgcgaggcca cgagcgtgct cgcgctgtag
     1801 ttcttcaagc attgatcgaa ctagccctgc tccgaaaagc atggacctgt atgctgaaaa
     1861 tcggctgatg cgacttttcg cgcatatccc ttccaatccg cttcggccca cctaagtaga
     1921 ccgcaacaaa tctctgcgcg atccccaagt tgtactgtcg ctgtctggga gactcctaag
     1981 cctaggaaag cgccgtgcgg cgtaaggttc gccgcggggg cgagcaggtt ttgcagacca
     2041 gtctaaaact tagacac
//
LOCUS       DAOSAI010000405         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_36063_length_2053_cov_4.193193, whole genome shotgun sequence.
ACCESSION   DAOSAI010000405 DAOSAI010000000
VERSION     DAOSAI010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36063_length_2053_cov_4.193193"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..>419
                     /locus_tag="PLM83_10670"
     CDS             181..>419
                     /locus_tag="PLM83_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK71906.1"
                     /translation="MTAAIQCNHASKSFRESVRNTENAGSDHNGQRALGRIVDFVMPS
                     RVTVEAVKDVSFEVERGEIFGILGPNGSGKSTLIR"
     assembly_gap    420..429
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <430..1063
                     /locus_tag="PLM83_10675"
     CDS             <430..1063
                     /locus_tag="PLM83_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK71907.1"
                     /translation="STLLIPDSGTVRVFGRDVVADRLEVRRYINRVSVEAAFFKKLSA
                     MENLEYAARLYGLEPREGLRRAREILARLGFPDSKVNVSLENLSRGMQQKVAIARALM
                     TAPVALLLDEPTTGLDPVSKRDVQDFVLEVCNTHDTTVILTTHDMEEADRLCDRIAVI
                     NNGEFVAVDSPENLKDRYANGNGHLPTLEEVFFRLTGRSPDEWEIEGDDQ"
     gene            1053..1880
                     /locus_tag="PLM83_10680"
     CDS             1053..1880
                     /locus_tag="PLM83_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71908.1"
                     /translation="MISNMQPGSVADYIESGYALVQRNYNLIKHEMGWEVVNAIYQVV
                     SALTIGFIGVSGPPEGMNERVLFLVSGALLWGFLSILFHEVAESVAWERWEGTIEYSF
                     MAPQPRGVYMFGVCMWGMLYGLVRVVICLTAVTLAFNISLAGANLGAAFVTLAASSLA
                     FIGMGLAAAVLPLISPEKGSQATHIFQAVVLLVSGVYYDIEVLPAWVRPLSYVSPATY
                     TLRAARAALLEGASVRTLLPDLLKLVILGAILIPVGFRIFSMGENYAMRTGKLKRTG"
BASE COUNT          440 a          506 c          618 g          479 t
ORIGIN      
        1 gggtcggctc gtctgagggt aggataatgc gctattgacg catttacagg ccgcgggcgt
       61 gcaaagcccg cggcctttta acgtttagcc tgcattacgc ttggccgcga agcttgcagc
      121 caattggcgc ccagcctaac tatcctgtag tcgagtcagc atcttaagga ggttattgac
      181 gtgactgcag cgattcagtg caatcacgca tccaagagct tcagggaatc cgtgcgcaat
      241 actgaaaacg caggttcaga ccataatggc cagagggcgc ttgggcggat cgttgacttt
      301 gtcatgcctt ccagggtgac tgttgaggca gtgaaggacg tgtccttcga ggttgaacgc
      361 ggcgagatat ttggcatcct agggcccaac ggttcaggaa aatccactct catccggatn
      421 nnnnnnnnna tcgacattgc tcatacccga ttccggaaca gtccgcgttt tcggcaggga
      481 tgttgttgca gacagactcg aagtgcgccg gtacatcaat cgcgtttcgg tagaagcggc
      541 atttttcaag aagctgtccg ccatggagaa tctcgaatat gccgcaaggc tctatgggct
      601 cgaacctagg gaagggctac gcagggccag agagatccta gcgcggttgg ggtttcccga
      661 cagcaaggtc aatgtgtcgt tggagaatct gtcccgcggg atgcagcaga aagtggctat
      721 agctagagca ttgatgactg ctcccgtggc attgcttctc gatgaaccca cgacagggct
      781 cgatccggtg tcaaaacggg acgttcagga tttcgtgctc gaggtctgca atacccatga
      841 cacaacggtc atactcacca cccacgacat ggaggaggct gataggttgt gtgaccgaat
      901 agccgtcatc aacaacgggg agttcgttgc ggtggacagc ccggaaaacc tgaaggatag
      961 atatgccaat ggaaacggac atcttcccac tctggaggag gttttctttc gattgaccgg
     1021 aaggagtcca gatgaatggg agatagaggg agatgatcag taatatgcag cctggatctg
     1081 tggctgacta catagaatcc ggctatgcat tggtgcagcg gaactacaac ctgatcaagc
     1141 acgagatggg ctgggaggtt gttaatgcca tctaccaggt tgtgagcgcc cttacgatag
     1201 gattcatcgg tgtgagcggg cctcccgagg gaatgaatga acgggtgctc ttcctcgtgt
     1261 cgggcgcgtt gctgtggggc tttctttcca tcctttttca cgaggttgca gagtcagtcg
     1321 cctgggagag gtgggaaggc accatcgagt attcgttcat ggccccgcaa ccgaggggcg
     1381 tgtacatgtt cggcgtttgc atgtggggaa tgctgtatgg gctggtgcgc gtagtgatat
     1441 gcctgacagc ggtcaccctt gcgttcaata tttcgcttgc aggggctaac ctcggggcgg
     1501 ctttcgtcac gcttgccgcg tcgagccttg ccttcatagg gatggggctg gctgcagccg
     1561 tgttgcctct gatatccccg gaaaagggct cacaggcgac tcacatcttt caggccgtgg
     1621 tgctgttagt gtcgggagta tattacgaca tagaggtttt gcctgcatgg gtcaggccgt
     1681 tgtcgtatgt atctcctgca acctacacat tgcgtgcggc cagagctgct ctgctcgaag
     1741 gcgctagtgt gaggacgctt ctgccggacc ttctcaaact cgtgatcctg ggggcgatcc
     1801 ttattccagt cgggtttcgg atcttctcca tgggcgaaaa ctatgcgatg cgcacgggca
     1861 agttgaaaag aacgggataa gctcccattg cagcagccta tgcacaggac agcaaaaggc
     1921 gcggacggga cggcacgcaa atcgccgtga cgtccgtgcc ctcgcttttc gagcagctaa
     1981 gtaactccca ataagttctt gcgctatcgg ttagcctgcc atatgatgtc tgctaggcgt
     2041 agacacgtct ttg
//
LOCUS       DAOSAI010000406         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_36399_length_2039_cov_3.970262, whole genome shotgun sequence.
ACCESSION   DAOSAI010000406 DAOSAI010000000
VERSION     DAOSAI010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36399_length_2039_cov_3.970262"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLM83_10685"
     CDS             complement(<1..380)
                     /locus_tag="PLM83_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOK71909.1"
                     /translation="MITFPADSDGSTAVSKLNPITKLIVLVSACTVALRLKEPLHALI
                     CFGLILIGHLLTKVRPAILWQRTRGIFMFGIILFASQIALVCDVPWQTRIANGALMSL
                     RFLNVVLASHLFVSVTSSEDFAYAL"
     gene            complement(355..1830)
                     /locus_tag="PLM83_10690"
     CDS             complement(355..1830)
                     /locus_tag="PLM83_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK71910.1"
                     /translation="MIDISGLRVRYYGSSELALRDIDLIVEPGESVIVTGPSGCGKST
                     LALCLAGFVPHAIGAEVAGSIRVCGINQREASVYRIAQHVGLVQQDPEGQFCTMCVED
                     EVAFGPENLRCSPYEVESRLDRALATTGVSHLRGRKLTELSGGEKQKVALAAVLAMEP
                     DLVILDEPTAYLDPRATRELIELVSRMRDADSRLTLLILEHKPWRFSHALERVIHLDR
                     GSLTYDGPIGGAAFEEEILDQLPHSHRSLHGFGEPLLRVQNLWQAYGERTVLRDVDLE
                     VCRGEIVGLMGDNGSGKTTLLHTIMGFLRPSSGRVLLEGDDITNKPVSARARRIGMIF
                     QNPNHQLFTDCVWREVVIGPLCQGDTEEECRPRADALLDSFGLSSYRDSHPLLLSYGQ
                     KRRLNLASTMASNPSLLLLDEPFAGQDVSRARDLMCILDELASEGVGVLIIGHDPDIM
                     TRCCDRILFMRDGKIQIDAPPYEAVAELERRGEHDYLPGRF"
     gene            complement(1823..>2039)
                     /locus_tag="PLM83_10695"
     CDS             complement(1823..>2039)
                     /locus_tag="PLM83_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HOK71911.1"
                     /translation="ILERAGVGESATEIQVVASDGYYAVFDLDDVMRRQDVILAQDDE
                     TLRLIATGYEGAYWVRMVSRIVVCEDD"
BASE COUNT          525 a          589 c          489 g          436 t
ORIGIN      
        1 agagcgtagg caaaatcctc cgagctagtg acacttacaa agagatggct tgccaggacc
       61 acattgagaa agcgcaaaga catcaaagct ccgttggcaa tccgagtttg ccaaggcaca
      121 tcacatacca aagcgatctg gctagcgaaa aggatgatgc caaacatgaa tatgcctctc
      181 gtccgctgcc acaagatggc cggtctcacc ttagtgagaa gatgcccaat gagaataagg
      241 ccaaaacaaa tcagggcatg caaaggctcc ttaagccgca aagctactgt acaagccgac
      301 actaatacga tcagtttagt gatggggttc agtttgctga cagcagttga gccgtcagaa
      361 tctgccggga aggtaatcat gttcgcccct cctttcaagc tcagctacag cctcgtaagg
      421 aggagcgtct atctgaatct ttccatcacg catgaaaagg atcctgtcgc aacacctggt
      481 catgatatcg ggatcatggc ctatgattaa gactccaacg ccctctgaag caagctcatc
      541 cagaatgcac atgagatcgc gggctcgcga tacatcctgc ccggcaaagg gttcatcgag
      601 caacaggaga cttgggttgg atgccatggt tgacgcgagg ttgagtcggc gcttctggcc
      661 gtagctcagt agtagcggat gggaatcgcg gtaactagag agtccgaatg agtcaaggag
      721 cgcatctgcg cgaggacggc actcttcttc agtgtcacct tgacataagg gacctatgac
      781 cacctccctc cacacacagt ccgtaaacag ctggtggttt ggattctgaa atatcattcc
      841 gattcttctg gcccgggctg ataccggctt gttggtgata tcatcccctt caagcagtac
      901 tctgcctgac gagggccgta agaatcccat aattgtatgg agcaaagtcg tcttgccgct
      961 tccattgtcg cccatcagtc ccacaatctc acctctgcaa acctccaaat ctacatcgcg
     1021 aagcacagtt ctctcgccat aggcttgcca gaggttctgc actctgagca aaggctcgcc
     1081 aaagccgtga agcgatctgt ggctgtgtgg gagctgatcc aggatctcct cttcgaaagc
     1141 ggcaccgccg ataggcccat cgtacgtgag agaacccctg tcaagatgga tcacccgttc
     1201 cagtgcatgg ctaaaacgcc atggtttatg ttccagtatc aagagtgtca acctactatc
     1261 cgcatcccgc atcctcgaca caagctcgat aagttcacgg gtggcacgcg gatcaagata
     1321 ggctgtaggt tcatccaaaa tcacgagatc aggttccatg gccagcactg cagcgagggc
     1381 aactttctgc ttctctcctc cagaaagctc agtcaacttg cgcccccgaa gatgagatac
     1441 tcctgtcgtt gccaacgccc tgtcaaggcg gctttcgacc tcataaggcg aacaacggag
     1501 attctcgggc ccaaatgcaa cctcatcctc cacgcacatg gtgcagaact gcccttcagg
     1561 gtcttgctgc acaaggccca catgctgtgc aatccggtaa acgctggctt ctcgctgatt
     1621 aatgccgcaa acccttatgc tgcctgctac ttcagcccca atggcatgag gaacaaatcc
     1681 tgccaagcag agcgccaatg tagacttgcc acagccgcta gggccagtca caatgacgct
     1741 ctcgccaggt tcaactatga ggtcaatatc tcttagagcg agttcggagg agccataata
     1801 ccgcactcta aggccggaaa tgtcaatcat cctcgcacac cacgatcctg gataccatcc
     1861 tgacccaata ggccccctcg taccctgtgg caatcaacct gagcgtttcg tcatcctgcg
     1921 ccaaaatgac atcttgacgg cgcataacat catcgaggtc gaacacggcg taatacccat
     1981 cagaagcaac aacctgaatc tctgtggccg actcgcccac gcctgcacgt tcaagaatc
//
LOCUS       DAOSAI010000407         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_36462_length_2037_cov_3.103431, whole genome shotgun sequence.
ACCESSION   DAOSAI010000407 DAOSAI010000000
VERSION     DAOSAI010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36462_length_2037_cov_3.103431"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PLM83_10700"
     CDS             complement(<1..129)
                     /locus_tag="PLM83_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK71912.1"
                     /translation="MSRIECDVAVIGAGPAGLAAAERAKQSGAGRVVLLDRDRELGG"
     gene            complement(122..1642)
                     /locus_tag="PLM83_10705"
     CDS             complement(122..1642)
                     /locus_tag="PLM83_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOK71913.1"
                     /translation="MAATKRITADVVVIGAGIVGTAIARELSKYELSVVLVEKEADVA
                     FGTTKANTALVHAGYDADPGTLKAKLNVAGNALYPKITQELGVLYKNTGSLVVALDED
                     QMLHIHELKIRGEKNGVPRLEVIPAERLLELEPHVNPDAVGALWAPTGGITCPWELAI
                     AYTENAVLNGARVLLSSPVIGIDVHEGRVTTVHCPNISIDARFVVNAAGVWADDIERM
                     AGRDDFEVTPRRGEYYLFDKRASRIVARPLFPVPTPFSKGIVVAPTVDGNLLAGPNSV
                     LTGRKDDYATTAPGLAEVLEGARKLIPDLPVREAITNFAGLRAVAEPGGDFIIGVSPG
                     LSNFINAAGIQSPGLTAAPAIAEMVRDILSDAGLELMEKPDFDPIRKRPLRFTELTRE
                     EQARMVAEDPKWGHIICRCETVTEAEIVAALHSPVPCTTVDGVKFRTRAGAGRCQGGF
                     CGPRGVAIIARELGIPVEKVTKKGGKSRIVMGPSKEPLLECSCSVEGTEKEVGDDV"
     gene            complement(1668..>2037)
                     /locus_tag="PLM83_10710"
     CDS             complement(1668..>2037)
                     /locus_tag="PLM83_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOK71914.1"
                     /translation="EAICYQTRDVLEAMTADSGIELKQLKVDGGAVANNFMMQFQSDI
                     LGVPVARPKVIETTALGAACLAGLGVGFWNGQDELACTWQRDREFTPKMCHGEREKLY
                     AGWKRAVERSRNWEQSISPC"
BASE COUNT          435 a          674 c          484 g          444 t
ORIGIN      
        1 tccgcccaac tcgcgatcgc ggtccaacag aaccacacgg ccggcgcccg actgtttcgc
       61 ccgttccgca gccgccagac cagccggtcc tgcacctatc accgccacat cgcattctat
      121 cctagacatc gtcgccgacc tccttctccg tgccctctac gctgcacgag cattcaagga
      181 gcggctcctt gctaggtccc atcacgatac gggatttgcc gcccttttta gttactttct
      241 ccacaggaat gcctagctcg cgtgcgatga tcgcaactcc cctcggcccg caaaagcccc
      301 cctggcacct tcctgcacca gcgcgagtgc ggaacttcac cccgtcaact gtggtgcagg
      361 gaaccggcga gtgcaaagcc gccactatct cggcctcggt cacagtctca cacctgcata
      421 tgatgtggcc ccatttcgga tcctccgcta ccatccgagc ctgttcttct cgggtcaatt
      481 cggtaaatcg gagcggacgc ttgcgaatgg gatcgaaatc gggcttctcc atcaactcca
      541 agccggcgtc tgacagtata tcgcggacca tctccgctat ggcaggcgcc gccgtaagtc
      601 cgggagactg tatgcctgct gcatttatga agtttgacaa accgggcgac actcctatga
      661 tgaagtctcc acccggttcg gctactgcgc gaagtccggc aaaattggta atggcctccc
      721 tcactggaag gtcaggaatg agcttcctag caccttccaa aacctcagcc aggcccggag
      781 ctgttgtggc ataatcatct ttcctgcctg ttaagactga attgggtccg gcaagcaggt
      841 tcccgtcgac tgtaggggca accacgatcc ctttggagaa cggcgtaggc actgggaaaa
      901 gcggccgtgc aacgatacga gaagccctct tgtcaaagag gtagtattct ccccgtctgg
      961 gtgtgacttc aaagtcatcc cggcctgcca tcctctctat gtcatcagcc caaactccag
     1021 cggcattcac gacgaacctc gcatctatag agatattggg gcaatggact gtcgtaacgc
     1081 gcccctcgtg cacatctatg ccaatcaccg gcgaacttag caatacccgt gccccattga
     1141 ggaccgcgtt ttctgtatag gcaatggcca actcccacgg gcaggttata ccgcctgtgg
     1201 gagcccagag cgcgccaacc gcatccggat tcacatgcgg ctcaagctcg agcaaccgct
     1261 ctgcaggaat gacctcgagc ctcggcacac cattcttctc tcccctaatc ttgagctcgt
     1321 gaatgtgaag catctgatct tcgtcaaggg ctaccaccaa cgagccggtg ttcttgtaga
     1381 gaacccctag ttcctgagtg atctttggat aaagagcatt gccggccacg ttcagcttgg
     1441 ccttcaaagt ccctggatcc gcgtcataac cagcatgaac aagggccgta ttggccttgg
     1501 tggtgccaaa ggccacatct gcctctttct caaccaacac aacactcaac tcatatttgg
     1561 aaagctcgcg ggctatggca gtgcctacaa tgcccgcgcc gataacaaca acatctgctg
     1621 taatccgttt cgttgccgcc atatcgttac cccctagatt ttccacctca acacggggaa
     1681 atactctgct cccaattgcg cgaacgctcc accgccctct tccaacccgc ataaagcttt
     1741 tcacgttcac catggcacat ctttggggta aactcccgat cccgttgcca tgtgcacgcc
     1801 agctcgtcct ggccgttcca aaagcccact cccaatcctg ccaggcaggc agcgcccaac
     1861 gcggtagtct caatcacctt gggcctggcc acagggacgc cgagaatatc agattggaac
     1921 tgcatcatga agttgttcgc caccgctccg ccgtccactt tgagctgctt aagctctatc
     1981 ccgctgtcgg cagtcatagc ctcaagcacg tcacgcgtct gatagcagat ggcctcc
//
LOCUS       DAOSAI010000408         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_36874_length_2022_cov_3.877987, whole genome shotgun sequence.
ACCESSION   DAOSAI010000408 DAOSAI010000000
VERSION     DAOSAI010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36874_length_2022_cov_3.877987"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PLM83_10715"
     CDS             complement(<1..1446)
                     /locus_tag="PLM83_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOK71915.1"
                     /translation="MRKWVMNIGLTWKFLLIFIVFAAIMGATAWVCMSTIDSFASDVD
                     EMASRYIPQLENALKAQTTAVSIDGYLAQLVMAEDYDTLTYYEHKIEEAKQAHEKNMR
                     IVQGRLKTPKGRGVYDELQGLWEEYLSFVAEVESLARMGAADTARFNMNNGQVITAEL
                     EGLHDQLVACVKGETDSVIERIQLNATRNLRRIVWITAIGALAGLAVGFPIGRSIAEP
                     ARKLAMLAPRVAAGDLTVDANSEAADEMGVLTRAFGGMIANLRNVVSESSAMARDVAA
                     ASQELSAGSEETAASIQEVTTTVRQIAGQAQEQSYSANETALSTAELGKSIRRVHEGA
                     ETQADVVSRTSAAYDEMGHSFDKTLILIDDVRLSTRRNVEAATDGCKVVDGLAARMAE
                     IDKKTIAASERISELEGLSHEIGRIVGVIEDIADQTNLLALNAAIEAARAGEQGRGFA
                     VVAEEVRKLAESSARETRAIGGLIQQVRESTE"
     gene            complement(1465..>2022)
                     /locus_tag="PLM83_10720"
     CDS             complement(1465..>2022)
                     /locus_tag="PLM83_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71916.1"
                     /translation="IVLLCEPFFPLWVIVLPHRFAVAGSLEEAKARPDGQFQDLRGLD
                     RAFCPRDAMDRTTTADTTTMDYTTMVGQAMGGRIDLPTSHTQDTMHSCKQSPPAIRSN
                     RSKTPYTPTPFVLMAPRVEDMSITGYSLRCGVELIQKALDIRNATWMPIIETRSIFGP
                     HSCLTLSSVLCVGYRDRRWCVCLPI"
BASE COUNT          451 a          599 c          474 g          498 t
ORIGIN      
        1 ttctgtcgac tctctaactt gctgaatgag gcctccaata gcccttgtct ctcttgcaga
       61 gctttcggca agctttcgaa cttcttcagc cacaactgcg aacccgcgac cttgttcacc
      121 tgccctcgct gcctctatgg cggcgttgag cgccaaaagg ttggtctggt ccgcaatatc
      181 ctctatcacg ccaacgatcc ttccgatctc gtgtgaaagt ccttcgagtt cgctgattct
      241 ttcagacgcg gcaatcgtct tcttgtcgat ctcggccatc cttgctgcca atccatccac
      301 cactttgcac ccgtcagtag cggcctcaac attgcgcctg gttgacagac gcacgtcgtc
      361 aattaggatc aacgtcttgt caaacgaatg gcccatctcg tcgtacgcag cagatgtacg
      421 gctcacaacg tcggcctgag tctcagctcc ttcatgtaca cgtctgatag actttcctaa
      481 ttcagctgtg gacaaagcag tctcattcgc cgagtacgac tgttcctgag cctgaccggc
      541 aatttgccgt actgttgtgg tcacttcctg aattgacgcc gccgtttctt ccgagcccgc
      601 cgaaagctcc tggctggctg cggcgacatc gcgagccatt gcagaagatt cagaaacaac
      661 gttgcgcaag ttggcaatca tgccgccgaa ggcccgcgtt agcacgccca tttcgtccgc
      721 agcttctgaa ttcgcatcta cagtcaaatc gcccgcggcc actctcggcg caagcatcgc
      781 cagtttcctg gctggctccg caatagaacg tcctatagga aacccgacag cgagacctgc
      841 gagggcacca atcgctgtga tccagacgat ccggcgaagg ttgcgagtag cgttaagctg
      901 gattctctcg ataacgctgt ctgtttctcc tttgacgcat gcaacaagct gatcatggag
      961 accttccagt tcagccgtga taacttgtcc attgttcatg ttgaatctcg ccgtatctgc
     1021 cgcgcccatc cttgcaagag actccacctc tgcaacgaaa gagagatatt cctcccatag
     1081 gccctgcaat tcatcataaa ccccacgtcc ctttggggtt ttgagtcgtc cctgaactat
     1141 tctcatgttc ttttcatggg cctgcttagc ctcttctatc ttgtgttcgt aatatgtaag
     1201 cgtatcgtaa tcctcggcca tcacaagctg agcaaggtaa ccgtcaatac tcacagccgt
     1261 cgtttgagcc ttgagcgcgt tttctagctg cggaatatat ctgctggcca tctcgtcaac
     1321 atccgaagcg aaagagtcta tcgtcgacat acatacccac gctgtagctc ccataatggc
     1381 cgcaaatacg atgaaaatca ggaggaactt ccatgtcaag ccgatgttca tgacccactt
     1441 tctcatctct ccacatatac ccccttaaat gggcaggcaa acacaccacc gcctgtctct
     1501 atagccaaca caaagaacag aactgagtgt cagacatgaa tggggcccaa atatggatct
     1561 cgtttctatt atcggcatcc atgtggcatt ccttatgtcc aatgctttct gtatcagttc
     1621 cacgccacac cgcagggaat atcctgtgat agacatatcc tcaacacgag gagccataag
     1681 cacgaacgga gtgggtgtat atggcgtttt actacgatta ctacgtattg ccggaggaga
     1741 ctgcttgcaa ctgtgcatcg tatcctgggt atgacttgtg ggcagatcaa ttcggccgcc
     1801 catggcctgg cccaccatgg tggtatagtc catggtggtg gtatccgccg tggtggtacg
     1861 gtccatggca tccctggggc agaacgcccg gtccaggccc cggaggtcct ggaactggcc
     1921 atccgggcgg gccttcgctt cctccagact tccagcgacc gcgaagcgat gaggtagaac
     1981 aataacccag agcggaaaga acggttcgca cagcaagaca at
//
LOCUS       DAOSAI010000409         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_36906_length_2021_cov_2.608850, whole genome shotgun sequence.
ACCESSION   DAOSAI010000409 DAOSAI010000000
VERSION     DAOSAI010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36906_length_2021_cov_2.608850"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLM83_10725"
     CDS             complement(<1..393)
                     /locus_tag="PLM83_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71917.1"
                     /translation="MPEKTVATVAKPAEAKGRGRMKLLKQWSVPIFFLALSIGGIAAA
                     GLDINFLIHEIISRFGRNMILVLSLLIPVMAGLGLNFGIVIGAMAGQTALIIITHYGV
                     EGLPGLLLAAVISVPLAILFGWLSGRILN"
     gene            complement(399..1472)
                     /locus_tag="PLM83_10730"
     CDS             complement(399..1472)
                     /locus_tag="PLM83_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71918.1"
                     /translation="MADTEEKSTSRITGFFSAAYKDFGLPRLIITGFVLFLFILATIM
                     KMDLTILISDSLVRIGMNGVLVLSMLPTLACGVGLNFGLPVGVIAGLIGAVMSMNWNL
                     QGFAGFFAAIAIALPVAMVAGYIYALVLERVQGQEMMVGTYVGFSIVSAMCMFWLLAP
                     IKNPAMIFAIGGQGLRYTLTLDKTFAKVLNDFLAFSVFGVTIPTGLLLFFGLLCVLMS
                     LFYRSKAGLAMRTVGSNPMFAKSSGMNVRYMKMWGVILSTVLAAVGIIVYSQSYGFVQ
                     LYTAPLYAAFPAVASVLIGGATLERATVGNAVIGTILFHTLLTIALPVTQTALRGADI
                     SEIARMIISNGMILYALTRAERR"
     gene            complement(1474..>2021)
                     /locus_tag="PLM83_10735"
     CDS             complement(1474..>2021)
                     /locus_tag="PLM83_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOK71919.1"
                     /translation="AFVSEDRRGVGLLLDESIEMNIALTAMQVQEKFLRPGAIPSLRL
                     ADEAAIREHALQVIKDLDIRCNGPEQLVRRLSGGNQQKICLGRAFTMQPEVMWVSEPT
                     RGIDVGAKQLVLDLLVDFNRKHGMTIIMTSSELGELRKVCDRIVIVYRGQIAGVLPPD
                     ASDVDFGLMMAGKGAARREVG"
BASE COUNT          513 a          589 c          496 g          423 t
ORIGIN      
        1 gttgaggatt cgtccactga gccatccaaa gaggatcgca agggggactg agatgacagc
       61 cgcaaggagg agacctggaa gcccctctac gccgtagtgg gttatgatga tcagagcggt
      121 ctgtccggcc atggcgccta tcacaatccc gaaattgagg cccaatcctg ccattaccgg
      181 gatgagaagc gacaggacca gaatcatgtt ccttccaaac ctcgatatta tctcgtgtat
      241 caggaagttt atgtcgagtc cggcagcggc gattccgccg atagatagcg caaggaagaa
      301 gatcggcacc gaccattgct tcaggagctt cattctgccc ctcccctttg cctcggccgg
      361 cttggctaca gtggcaactg tcttctcagg cacgccggct acctcctttc ggcccgggta
      421 agggcataca ggatcatgcc gttgcttatg atcatccggg ctatttcgct gatatcggcc
      481 cctcgcaagg cggtttgggt aaccggaaga gctattgtga gcagcgtgtg gaaaagaatc
      541 gtccctatta cggcatttcc cactgtagcc cgttccagcg tagccccgcc tatcaggaca
      601 gaagcaacag ccggaaatgc agcatacagc ggcgctgtat aaagctgcac aaacccataa
      661 ctctgggaat agacgatgat gcccaccgcc gcaagcaccg tcgagagaat gacgccccac
      721 atcttcatgt atctaacgtt cattcccgaa gatttcgcga acatgggatt tgaccccact
      781 gtccgcatgg caaggcctgc tttggaccgg taaaacaggc tcatcaagac gcaaagaagt
      841 ccgaagaaaa gcaaaagccc ggtgggaatc gtcactccaa aaacactgaa ggccaaaaag
      901 tcgttgagca cctttgcaaa ggtcttgtca agagtgagtg tgtaccggag cccctgccct
      961 ccaatagcga atatcatggc cgggttcttg ataggtgcca agagccagaa catgcacatc
     1021 gccgatacta tagaaaaccc cacgtacgtc ccgaccatca tctcctgtcc ctgaacccgt
     1081 tcgagaacaa gagcataaat gtagccggca accatggcaa ccggcagagc gatggcaata
     1141 gctgcgaaga acccggcaaa cccttgaagg ttccagttca tgctcattac cgccccaatt
     1201 agtcccgcta ttactcccac aggcagtccg aagttgagcc caactccaca tgctagcgtg
     1261 ggcaacatcg agaggacaag cactccgttc atgcctatac gtacaaggga gtcgctgata
     1321 aggatagtca ggtccatctt catgatagta gccaggatga acaggaacaa cacgaacccc
     1381 gtgattatga ggcgaggaag tccaaaatcc ttataggcag cggaaaaaaa ccccgttatg
     1441 cgactcgtgg atttctcctc cgtatcagcc atgtcaacca acctcccttc gcgcagcacc
     1501 cttgccagcc atcataaggc cgaagtccac gtccgaagca tccggcggga ggacacctgc
     1561 aatttgcccg cgatatacta tcacgatccg gtcacatacc tttcttaact ccccaagctc
     1621 agacgatgtc atgatgattg tcatcccatg cttgcggttg aaatccacga gcagatcgag
     1681 aacgagctgc ttcgcgccta catcgattcc cctagtcggt tcggagaccc acattacctc
     1741 aggctgcatg gtaaatgccc ttccgagaca gatcttctgc tggttcccac cagacagcct
     1801 tctaacaagc tgttcgggtc cattgcacct gatatcgaga tcttttatga cttgaagcgc
     1861 gtgttcgcgt atcgctgctt catctgccag cctgagcgag gggattgctc ccggtctcaa
     1921 gaacttctct tgcacctgca ttgccgtgag agcgatattc atctcgatag actcatccag
     1981 gagcaatccg acgccgcgcc tgtcctcgga aacgaaggcc a
//
LOCUS       DAOSAI010000410         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_37453_length_1999_cov_3.452160, whole genome shotgun sequence.
ACCESSION   DAOSAI010000410 DAOSAI010000000
VERSION     DAOSAI010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37453_length_1999_cov_3.452160"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1367)
                     /locus_tag="PLM83_10740"
     CDS             complement(198..1367)
                     /locus_tag="PLM83_10740"
                     /inference="COORDINATES: protein motif:HMM:NF017328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination C-terminal
                     domain-containing protein"
                     /protein_id="HOK71920.1"
                     /translation="MSLRRMSALVCAVILVALLMPFASGCWDSIDIEDRSFVAVAALE
                     PAEGGENKGVSVSILIPIPGGAQGGANGGGRGNGQDNAPKSVVIKENGLYIEDAVEKM
                     RAKFGGVIDFSSLQFIAIGKDVDPEDMWKGLSTVLFFRYVPLTSLIFATEDDMGELLR
                     LSPASGKRLVEVLNGLEAQSKQGLGSVRFLSVWQVMSLIRNDVGDVVLPLIGLTDGGD
                     GIEALGTAVYDGTRRAGTLGPTDSMLLYGIAWRKHLGGTYTMHYEGREFIVRIIRMKT
                     KIRMTGVDANGLPRVGVKLSVAARLLDSGGYRVSLVDRSRLNDLERAAERDLEDRLER
                     ILMKLKEWNSDAMRLYYALRLKTPYMSYDDFKGVYSKMEVDFAVSMSLRRAGLLK"
     gene            complement(1368..>1999)
                     /locus_tag="PLM83_10745"
     CDS             complement(1368..>1999)
                     /locus_tag="PLM83_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71921.1"
                     /translation="LEDKLDLSHLLPLRASELAADPFGLMIAATSMYHGYTVILIAGP
                     AFNPPGRSALLAATVGTLLAVPGFVTFSAYPIAAFGWPLAGDFAYPAGSFLEVLAPGG
                     QGFPVRRFDFILTMFFRFIMIIASITYFFSAAQTLSDLVSPKAEKIHPVLVLALGALI
                     GFSFVVLPDLSSLLAFVRIWLALGAILTIVTIILAVRSYLATTRAGGDE"
BASE COUNT          517 a          576 c          475 g          431 t
ORIGIN      
        1 acaagacgaa ctccgtttcc cgaacacgcg gtgatgatct gcctgatttt cgtcatcggt
       61 tcggcctcct ttctgatctc acggttggga taatcaaaca tggtaagtat aacgtggaag
      121 ggatatcggc gtcaagcgaa aaatccactc gcataacgca tgtccattgc tctgctcagg
      181 cacggcattt ccgccgctca cttgagcaac cctgcgcgac gcaagctcat cgaaacggca
      241 aaatcgacct ccatctttga atatacgccc ttgaaatcgt catatgacat atacggcgtc
      301 ttcagcctca gtgcatagta taacctcata gcatcggagt tccactcttt gagcttcatc
      361 agaattctct ccagcctgtc ctctagatcg cgctcagcgg cgcgctcaag atcgttcaat
      421 cggctccgat cgaccagcga aactcggtag cccccagaat cgaggagccg agcggcaaca
      481 gacagcttta cgccgacgcg gggcaaccca ttcgcatcga cgcctgtcat gcggattttg
      541 gtcttcatgc gtatgatcct cacgatgaat tcccgtccct cgtagtgcat ggtgtatgtg
      601 ccacccaagt gtttgcgcca ggctatgccg tatagcagca tcgaatctgt aggcccaagc
      661 gttcctgctc gccttgttcc gtcatataca gctgtcccta gggcctcaat tccatcgcct
      721 ccatcggtga gaccgatcaa cggcaagaca acatcgccaa cgtcatttct gatcaaggac
      781 atcacctgcc atacggagag gaaacgaaca ctccccaacc cctgcttgct ctgtgcctct
      841 aagccattga gcacttccac aagtctcttt ccgcttgcag gagataaacg aaggagttcg
      901 cccatgtcgt cttctgttgc aaatatcaag ctggtcagcg gcacgtatcg aaagaagaga
      961 accgtgctca gccccttcca catgtcttca ggatctacgt ctttccctat ggcaatgaac
     1021 tgcaaggatg aaaagtctat gacccctccg aatttggcgc gcatcttctc tactgcatcc
     1081 tctatataca gcccgttctc cttgatcacc acgctttttg gggcgttatc ctgtccgttg
     1141 cccctcccgc ctccattagc accgccttgc gctccccctg gaattgggat cagaatactg
     1201 acagagacac ctttgttttc gccaccttca gcaggttcca aagcggcaac tgcaacgaaa
     1261 ctgcggtctt caatgtcaat cgagtcccag cacccggatg caaaaggcat taaaagggca
     1321 acaaggatga ctgcacatac gagtgcagac attctgcgta agctcaccta ctcatcaccg
     1381 ccagcacgcg tcgtagcgag ataagaccga acggccaaga taatcgtgac aatagtaaga
     1441 atggctccca gcgcaagcca aatgcgcaca aacgccaaga gagaactgag atctggcaat
     1501 acaacaaagg aaaagcctat caacgccccc aaagctagca ctagtacagg atgaatcttc
     1561 tccgctttgg gagacaccaa gtcgctcaat gtctgtgccg cgctgaagaa ataagtaatg
     1621 gatgcgatga tcatgatgaa acggaagaac atggttagga tgaagtcgaa acgccggacc
     1681 gggaatccct ggcctcctgg tgccagaact tccaggaacg agccggcagg gtatgcaaaa
     1741 tcgccggcta aaggccagcc gaatgcggca atgggatagg cagaaaaagt tacaaacccc
     1801 gggactgcca ggagggtgcc aaccgtggca gctaacaatg cgcttcgtcc gggcgggtta
     1861 aacgcaggcc ctgcaattag aattacggtg taaccatggt acatagatgt cgcagctatc
     1921 ataaggccaa acggatcagc tgccagttcc gatgcgcgca atggcaatag gtgcgacaga
     1981 tcgagcttat cttcgagga
//
LOCUS       DAOSAI010000411         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_37851_length_1984_cov_4.273199, whole genome shotgun sequence.
ACCESSION   DAOSAI010000411 DAOSAI010000000
VERSION     DAOSAI010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37851_length_1984_cov_4.273199"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /locus_tag="PLM83_10750"
     CDS             <1..1759
                     /locus_tag="PLM83_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOK71922.1"
                     /translation="QQTVWLKRKVDHEAAMEVRRLSLPGIAVAESGQRFYPKDTLAAH
                     VIGIAGIDNQGLEGLEQYYDAELRGVRGQIVAERDATGREIPEGRHSYVPPTDGNSLV
                     LTIDEVIQYICERELEIAIAETGSTRGLILAMDPKTGEILAMAMRPTFNPNSYGKYPS
                     EFRRNFIVSDMLEPGSTFKVITAAAALEEGIVTASTPFYDPGYLKVEDRTIRCHKPGG
                     HGSETFMEALENSCNPVFATLALRLGVEKFVQYARAFGLLEKTGVDYPGEATGMMHQI
                     SKVGPVELATYGFGQGISVTPLQLLNAMCAIANGGALMRPMLVRKVIGADGALVEEHV
                     PTKVRQVISSSTASELSRMLQSVIVNGSGQRGAVPGYAVAGKTGTAQKPERGRYGDKR
                     IASFMGYGPASDPKVAVLVILDEPSSDVKYGGVIAAPVFRAVMQDIFRYMEIPPSYAV
                     GMPQGTAGSGNSVFVPDVTGKSRAEAEAGLKEVGLLWRHEGIGDVVTNQVPKAGSQVG
                     AGTTVIVYFGHEELYNREDVEIQVPDVIGKTLKEAALVLGARGLGVDPSGKGLVVRQT
                     PAPGTHVKKGAIVTIFLEE"
     gene            1765..>1984
                     /locus_tag="PLM83_10755"
     CDS             1765..>1984
                     /locus_tag="PLM83_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOK71923.1"
                     /translation="MPHNSLESLLQGVSHELSGDPSTEITSLSYDSRKVDGQGTLFAA
                     FVGARFDGHRFIDEAVRRGASAVLVSEPV"
BASE COUNT          452 a          496 c          627 g          409 t
ORIGIN      
        1 ccagcagacg gtttggctaa agcgaaaggt agatcacgag gcggctatgg aggttcgtcg
       61 gctctcactg cccggaattg ccgtggcaga aagcgggcag aggttctatc ccaaggacac
      121 gcttgccgct cacgtaatcg ggatcgccgg gatcgacaac cagggcttgg aagggctgga
      181 gcagtattac gatgctgagc ttaggggcgt ccgaggccag atagtggccg aaagagatgc
      241 gaccggtcgc gagattcccg aaggccgcca ctcatacgtg ccccctactg acggaaatag
      301 cctcgttctg actatcgatg aagtaataca gtacatatgc gagcgagagc tcgagatcgc
      361 gatagcggag actgggtcca cgcgtgggct catccttgcc atggatccga aaactggaga
      421 aatcctggcc atggcgatga ggcccacatt caacccgaac agctacggaa agtacccgag
      481 cgagttcagg cgcaacttca tagtcagtga tatgttggag cctggatcta cgttcaaggt
      541 tattaccgcc gctgctgccc tcgaggaggg catcgttacc gcctcaaccc cattttacga
      601 tccgggatat ctcaaggttg aagaccggac cattagatgc cacaagccgg gtgggcatgg
      661 ttcggagaca ttcatggaag ccctcgagaa ttcctgcaac cccgtcttcg ccacgcttgc
      721 gttaagactc ggggtcgaaa aattcgtcca gtacgctcgc gcgttcggac tactcgagaa
      781 gacgggagta gattacccgg gggaagctac agggatgatg caccagatat cgaaggtagg
      841 accggtggaa cttgcgacct acgggttcgg ccagggcata tccgtcacgc cattgcagct
      901 tctgaatgcg atgtgtgcca tagccaacgg cggggcgctg atgagaccca tgttggtgag
      961 gaaggtaatc ggcgctgatg gggctctggt tgaggagcat gttcccacaa aagtgcggca
     1021 ggtgatatct tcttccaccg catcagagct ttcgaggatg ctccaatctg taatcgtcaa
     1081 cggctccggc caacgcgggg ctgtgccagg ttatgctgtg gcagggaaga cgggtactgc
     1141 gcaaaagccc gagcgtggac ggtacggtga caagaggatc gcatcgttta tgggttacgg
     1201 cccggcaagc gaccccaagg tcgcagtgct tgtcatcctc gatgagccca gctcggatgt
     1261 gaaatacggt ggagtgattg ctgccccggt cttccgcgcg gtgatgcagg acatctttag
     1321 atacatggag attccgcctt catacgccgt tggaatgccc cagggcacag cagggtcggg
     1381 caatagtgtt ttcgtgcctg acgtgaccgg aaaaagtcgg gctgaggctg aggccgggct
     1441 taaggaagtc ggtcttctgt ggaggcacga ggggattggc gatgtcgtaa cgaaccaagt
     1501 gcctaaggct ggttcgcaag ttggggcggg aaccacggtc atcgtttact ttggccatga
     1561 ggaactgtat aatcgtgaag acgttgagat acaggtaccg gacgtaattg ggaaaactct
     1621 aaaggaggcg gcccttgttt tgggcgcccg tggccttggc gtggatccgt caggaaaggg
     1681 cctggtagtt cgacagacgc ccgctccggg aacgcatgtc aaaaagggcg cgatcgttac
     1741 gatattcctt gaggaatgat gtgtatgcca cataacagct tggaaagttt gctgcaaggt
     1801 gtttcgcacg agctttcagg agatccaagt accgagataa caagcctctc atacgattct
     1861 cgaaaggtgg acggacaggg gactctgttc gcggcattcg tcggggcgcg attcgacggg
     1921 caccgcttca tcgacgaggc ggtcaggaga ggcgcttctg cagtacttgt gtcagaacct
     1981 gtac
//
LOCUS       DAOSAI010000412         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_38022_length_1978_cov_4.794072, whole genome shotgun sequence.
ACCESSION   DAOSAI010000412 DAOSAI010000000
VERSION     DAOSAI010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38022_length_1978_cov_4.794072"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PLM83_10760"
     CDS             complement(<1..121)
                     /locus_tag="PLM83_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71924.1"
                     /translation="MVDAIVIKIRKGGRVWNHSALIAVGVALGSCLQIVRRKTR"
     gene            complement(114..356)
                     /locus_tag="PLM83_10765"
     CDS             complement(114..356)
                     /locus_tag="PLM83_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOK71925.1"
                     /translation="MNSHIFTAIASYLPSNMFIALIATLVEMVVDGVSTRKVCKITRE
                     LCGAEVSKSTVSAMVTCSARMARAFSRQRAISISNG"
     gene            complement(581..1723)
                     /locus_tag="PLM83_10770"
     CDS             complement(581..1723)
                     /locus_tag="PLM83_10770"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK71926.1"
                     /translation="MKVNLARPDITQAEIDAVLDVLNSYTLALGPKMMEFEMAIASYC
                     GRKHGIAVNSGTSGLHLLVRAYDIGQGDEVITTPFSFIASSNCILYEGAKPVFVDIEP
                     DTANIDPDLIEAAITPKTKAILAVDAFGQPAKVDVIREIARRNDLVFIEDSCEAIGSH
                     YKGKPAGGPDFADAAVFAFYPNKQITTGEGGMVVTDDDRIAFLCSSMRNQGRGESGVW
                     LAHERLGYNYRMDEMSAAIGVVQMQRIEEIIEKRAAVAKMYKERLSGITGVRLPHIAQ
                     EVTRMSWFVYVIRVGWDEETPKMQSKVRDHVMARLGEADIGCRPYFTPIHLQPFYREM
                     FGYQEGDFPVTEELGRTSIAIPFYNNLTEDEIDYVGQVIERALEER"
BASE COUNT          454 a          548 c          469 g          507 t
ORIGIN      
        1 cacgagtctt ccttctcact atctgcaaac atgaacccaa ggctacgccc acggcgatca
       61 gtgcgctgtg attccaaacc ctgcctccct tacggatctt aatcactatg gcatcaacca
      121 ttagaaatgg atatggccct ttgtcgagag aacgctcgcg ccattcgcgc actgcaggtc
      181 accatggctg agactgtcga tttcgagact tctgcaccac atagctcgcg ggttatcttg
      241 cagaccttac gagtagacac tccgtctaca accatctcga caagcgtggc aatcaaggca
      301 atgaacatgt tcgatggtag gtatgaggca attgctgtga agatatgaga gttcactgaa
      361 aactccttat tgagcaccga aaacatgaat gaaaggggta tcaattccct ggaatgttct
      421 ggtaacagct tcgggtcatc gagtagcgag cggttagccc ggctccaata atgtttgtgg
      481 gcaaacgctc caggcacccg ttgatatccg ggtcgcgtct gcccacatct acatcattta
      541 tggaaggctt tcagggggac ccacgggagg tcgccaacct ctacctctct tcaagcgccc
      601 tctcgataac ttggcctacg taatcgatct cgtcctctgt aaggttgttg tagaatggta
      661 tggctatgct agtgcgcccc agctcttcag taaccgggaa atcgccttct tgatatccaa
      721 acatttccct gtagaacggc tgcaagtgta tgggtgtgaa gtatgggcgg caaccgatat
      781 cagcttctcc gagccttgcc atcacgtggt ctctcacttt ggactgcatc tttggggttt
      841 cctcatccca tccaactcgg ataacgtata caaaccagct cattctggtg acttcctgag
      901 ctatatgagg caagcgaacc cctgttatcc cgctcagccg ctccttatac atcttagcca
      961 cggcagcgcg tttctcgatg atctcttcta tgcgctgcat ctgcacaacg ccaatcgccg
     1021 cagacatctc atccattctg tagttatatc ctaagcgttc atgtgccagc cacacgccgc
     1081 tctctcccct gccttgattg cgcatgctag aacagaggaa tgcaatgcga tcatcatctg
     1141 taacgaccat gcccccttca cccgtggtaa tctgcttgtt gggatagaaa gcgaagacgg
     1201 ccgcatccgc gaagtcgggc ccccccgccg gcttgccctt gtaatgggat ccaatagcct
     1261 cacatgagtc ctctatgaat acgagatcgt tccgtctggc gatctcgcgt atgacgtcca
     1321 cttttgcggg ctggccaaag gcatccactg ccaggatggc ttttgttttt ggcgtaatcg
     1381 ccgcttcgat gagatcagga tctatgttgg cagtgtcggg ttctatatcg acgaaaactg
     1441 gttttgctcc ctcatacaaa atgcaattgc tggaggcaat aaagctgaat ggggtagtga
     1501 tgacctcgtc gccctggcca atgtcgtacg cgcgcaccag aagatgaagg ccgcttgttc
     1561 cactgtttac agcaatgcca tgttttcgcc cacaatagct tgcaatagcc atctcaaact
     1621 ccatcatttt cggtccgaga gccaatgtgt atgaattgag cacgtccagc actgcatcga
     1681 tttcagcctg cgtaatgtca gggcgggcaa ggttcacttt catgactgtc cctccatgtt
     1741 gtctttgtag ttcaatgtta tagattctca actcaatggg gaacacctgc ttgcgccctg
     1801 cgattatcgc ttcagccatc tgtatagcat ttaccctttg tgggtcagga ttccgggcgg
     1861 tcttgaaaag tcaaatgagg atccatagca tgccggacaa caatatcgcc tagcacagag
     1921 cagatcgagt agggtggcgc caggtaatca gcctggcgtc atccctctca cagaaccg
//
LOCUS       DAOSAI010000413         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_38061_length_1977_cov_2.843392, whole genome shotgun sequence.
ACCESSION   DAOSAI010000413 DAOSAI010000000
VERSION     DAOSAI010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38061_length_1977_cov_2.843392"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1218
                     /locus_tag="PLM83_10775"
     CDS             103..1218
                     /locus_tag="PLM83_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOK71927.1"
                     /translation="MAKRSVLAAVALALILGLASVSIGAADFKIGVVTGTVSQGEDEY
                     RGAEAAIQKYPGLILHRSYPDNFMQEQETTIAQITGLASDRQVKAIVINQAVPGTVAA
                     IRKVREIRPDIIFVLGEPHEDPPIVEKYADVSLIPNNIARGTTIVRLAKKLGAKTFVH
                     YSFPRHMSYADLATRRDIMEEECKKLGMRFVFANAPDPMGEGGLPAAQQFILEDVPRK
                     VGQYGKDTAFFSTNCGMQEPLITAVIKAGAIFPEQCCPSPTHGYPGALGLEITDNMKG
                     NFPAIIRAINAEIVKKGQAGRMATWPVATGYLNTIAGVEIAKLAVEKKLNYKDMDAVR
                     KVVQGIAGTPIEFERLSAGGQFYRYLIDSIIFGVTVK"
     gene            1318..>1977
                     /locus_tag="PLM83_10780"
     CDS             1318..>1977
                     /locus_tag="PLM83_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK71928.1"
                     /translation="MAITEGNVLELEGISKSFFGTQVLKDVNLSVRPGEIHALVGENG
                     AGKTTLMNILFGMQVIFETGGFEGRIILDGVPTDISSPREAMTYGIGMVHQEFMLIPG
                     FTVAENIKLNREPTLPNLASKIFGQKLETVDEEAMRADARAALDKLAVSIDELALVAG
                     LPVGHMQFVEIAREIDKHSVELLVFDEPTAVLTESEAQMLLDAMKRLASEGIAILFIT
                     HR"
BASE COUNT          475 a          494 c          589 g          419 t
ORIGIN      
        1 aaaggacaat aaggcaatag aatacttttc acacgacatt acttcgttcg gagggcgcat
       61 gttgcgcccc caaaaatact tctgcagggg gagaatagcg aaatggcaaa aagaagtgtc
      121 ctcgcagccg tagcgctggc cctgattttg gggcttgcta gtgtctctat tggggccgct
      181 gacttcaaga tcggcgtagt tacgggaacc gtatcgcagg gcgaagacga ataccgaggc
      241 gctgaggccg ccatacagaa gtatcctggg ctcattctgc atcggtcgta ccccgacaat
      301 ttcatgcagg agcaggagac gactatagcg cagattaccg gactcgcctc tgacaggcag
      361 gtcaaggcga tagtaatcaa ccaggccgtt ccgggcactg tggctgctat ccgcaaggtt
      421 cgcgagataa ggccggatat catcttcgtt ctgggcgaac cgcatgaaga ccccccgata
      481 gtcgaaaagt atgcagatgt ttccctgatt cccaataata tagctcgtgg aaccaccata
      541 gtccggctgg caaagaaact aggtgcgaag acgttcgttc actattcatt cccgaggcac
      601 atgtcctatg ccgatctggc gacgcggcgg gacatcatgg aggaagagtg caagaagctt
      661 gggatgaggt ttgtgttcgc aaacgcgcct gatcccatgg gtgaaggcgg actccctgcg
      721 gcgcagcagt tcattctgga agatgtgccc agaaaggtcg ggcagtatgg taaggacacg
      781 gcgttctttt cgaccaactg cggcatgcag gaacccttga taactgcggt catcaaagcc
      841 ggcgccatat tccccgagca gtgctgcccc agcccgaccc atggctatcc aggtgctctc
      901 ggtctggaga tcacggacaa catgaagggg aatttccccg caataatccg ggcgatcaac
      961 gccgagattg tgaagaaggg tcaggcgggc aggatggcga catggccggt cgctactggt
     1021 tacctcaaca ccattgctgg tgtggaaatt gccaagctgg cagtggaaaa gaagctcaat
     1081 tacaaggaca tggatgccgt aagaaaggta gttcagggga tcgctggcac accaatagag
     1141 tttgagagac tcagcgcggg cgggcagttc tatcgctacc tgatcgattc gatcatcttt
     1201 ggcgtcacgg tcaagtagtc ggcgaggcct actttagcag cgaaattggc gccgggcaca
     1261 gctaccgccc ggcgcctgcc cgtaatttcc tttcatgcta acgagagggg cttgacaatg
     1321 gcgataactg aagggaatgt tcttgaactt gagggcataa gcaaaagttt cttcgggacg
     1381 caagtgctca aagacgtcaa tttgtcggtc aggccgggag agatacatgc tcttgtcggc
     1441 gagaacgggg ccggaaagac tactctgatg aatatcctct tcggcatgca ggttatcttc
     1501 gagactgggg gctttgaagg ccggattatt ctggacggcg ttccgacgga tataagctct
     1561 cctcgggagg cgatgaccta tggcataggc atggtgcatc aggagttcat gctgatcccg
     1621 gggtttactg tggccgagaa tatcaagctc aaccgggagc cgacgctgcc gaacctcgca
     1681 tcgaagatct ttgggcaaaa acttgagaca gttgacgagg aagctatgcg agccgatgcc
     1741 agggcggccc tcgacaagct ggcagtctcc atcgatgagt tggcgcttgt ggcaggtctg
     1801 cctgtgggac acatgcagtt tgttgagata gcgcgggaga tcgacaagca cagcgttgag
     1861 cttcttgtct ttgacgaacc cacggcagtc ctcacagaga gcgaggcgca gatgctcctc
     1921 gatgccatga agaggcttgc gtctgaggga attgccatac ttttcatcac tcaccgg
//
LOCUS       DAOSAI010000414         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_38198_length_1972_cov_4.509129, whole genome shotgun sequence.
ACCESSION   DAOSAI010000414 DAOSAI010000000
VERSION     DAOSAI010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38198_length_1972_cov_4.509129"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLM83_10785"
     CDS             complement(<1..651)
                     /locus_tag="PLM83_10785"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781606.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOK71929.1"
                     /translation="MTKAHQLLYDELGRSFSGKDFLSIHDFTSEQIMRVMELSALLKA
                     EQKAGVRHHLLEGKTLGMIFLKPSTRTRVSFETAIWQLGGYGLFLSSNDLQIKRGEPV
                     PDTARVLSRYLDAIMIRTFGHDEVVELAEWASIPVINGLTDLLHPCQVMADVFTGIER
                     FGDLRGRKLTYIGDGNNMVHSLMYAGAKLGMHVTCACPDQYQPDAAVMEAAKADAVA"
     gene            complement(665..1672)
                     /gene="amrS"
                     /locus_tag="PLM83_10790"
     CDS             complement(665..1672)
                     /gene="amrS"
                     /locus_tag="PLM83_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOK71930.1"
                     /translation="MHARVEDSLGICELCPRRCKLQEGATGACRVRTMIGGRIIPKNY
                     GAITSMAIDPIEKKPLYHFMPGSGVLSVGTFGCNLSCRYCQNWEISQRHAESRFFTPD
                     QLADLAESMREQSVGVAYTYSEPFVWYEFVYDCAKLVHEIGLSNILVTNGYINPEPLA
                     GILPYVDAINVDLKGYDDEYYRKVCGGTLAPVISTIRAVYESGCHLELTTLVVPGYND
                     SPEEMEQLASWVASLSPEIPLHISRYFPSYRMNEPPTPMATMEKCVEVAKRRLNYVYV
                     GNASIPGGSDTVCPNCGELLIERRGFACVTVRLEGVSCPKCGHKIPVRLAGGESAGNK
                     Y"
     gene            complement(1680..>1972)
                     /locus_tag="PLM83_10795"
     CDS             complement(1680..>1972)
                     /locus_tag="PLM83_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMMECR1 family protein"
                     /protein_id="HOK71931.1"
                     /translation="AAVRDPRFEPVAPHELDDIVYSVDVLSPPEPVSGPEELDPQRYG
                     VIVRNGGRVGLLLPNLEGINTVEEQISIARRKAGIGLNAPVELERFEVTRYE"
BASE COUNT          478 a          570 c          458 g          466 t
ORIGIN      
        1 ggcaactgca tcggcctttg cagcctccat tacggcagca tcgggctggt attgatcggg
       61 gcatgcgcac gtaacatgca tccccagttt ggcgccggca tacatcaagg aatgaaccat
      121 attgtttcca tcgcctatat acgtaagctt gcggcctctg agatccccaa atctttcgat
      181 tccggtaaac acatcggcca tcacctggca cggatggagc aagtcggtaa gtccattgat
      241 gacgggaatg gatgcccatt cagccaattc aacaacttca tcgtggccaa aagtcctgat
      301 cattatggcg tcaaggtagc gcgacaacac ccgcgctgtg tcaggcacag gttcaccccg
      361 cttgatctga agatcgtttg aactgagaaa aaggccataa ccgcccagct gccatatggc
      421 cgtctcaaac gagaccctcg ttcgcgtcga tggtttcaag aatatcatgc ctaacgtctt
      481 tccctcgagg agatgatggc gcacccccgc cttctgctct gccttgagaa gcgccgacaa
      541 ttccatgacc cgcattatct gctcactagt gaaatcatga attgatagga agtccttccc
      601 tgaaaagctg cgcccaagct cgtcgtagag tagttgatga gcctttgtca tctttccacc
      661 gccttcaata tttgtttccg gcgctctcgc ccccagcaag cctaacagga atcttgtggc
      721 cacatttggg acacgaaact ccttcaagtc gaacagtgac acaggcgaat ccgcgcctct
      781 ctattagtag ctctccgcag ttggggcaga ccgtatcgct gccgccaggg atgctggcat
      841 tccctacata cacataattc agtcgtctct tggccacctc cacacatttc tccattgttg
      901 ccatgggcgt tggcggctcg ttcatcctat agctaggaaa atacctcgat atgtgcaacg
      961 gaatctccgg cgaaaggctt gcaacccacg aagcaagttg ttccatctct tctggagaat
     1021 cgttatagcc tggcactacc agcgtagtca attcgagatg gcaacctgat tcgtacacag
     1081 cacgaatcgt tgagattacc ggcgcgagag ttcctccgca cactttccgg taatactcat
     1141 catcgtatcc tttgagatcg acattgatcg catcaacgta aggcaatatt cccgccaatg
     1201 gctcagggtt gatgtagccg tttgtgacga ggatattgga aagcccgatc tcgtgcacga
     1261 gtttagcgca atcatacaca aattcgtacc aaacgaaagg ctcagagtag gtgtaagcaa
     1321 caccaactga ctgctcccgc atgctttctg ccaagtccgc caactggtcg ggagtaaaga
     1381 atctggattc tgcgtgtcgt tgcgatattt cccagttttg gcagtatcta catgacaggt
     1441 tgcaaccgaa cgtccccaca gacaagactc ccgagcctgg catgaaatga tacagaggct
     1501 tcttctctat agggtctatc gccatagaag tgatagcgcc gtagttcttc ggaatgatcc
     1561 gtcctccgat catggtccga acccggcatg ctcccgttgc accctcttga agcttgcatc
     1621 tacggggaca cagctcacaa ataccgagcg aatcttctac cctggcatgc atagcctctc
     1681 tattcgtatc tagtcacctc aaaccgctca agttctacag gcgcattcag acctataccg
     1741 gccttacgcc gcgcaataga gatctgctcc tctactgtgt taatcccctc gaggtttgga
     1801 aggagaagcc ccacccgtcc cccattcctc acaatgactc catatctttg gggatccagt
     1861 tcctcaggac cggagacagg ttccggaggg gaaagaacgt ccacagagta tacaatgtca
     1921 tccaactcgt gaggagcaac cggctcgaaa cgaggatcgc gcacagccgc gg
//
LOCUS       DAOSAI010000415         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_39026_length_1944_cov_3.317628, whole genome shotgun sequence.
ACCESSION   DAOSAI010000415 DAOSAI010000000
VERSION     DAOSAI010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39026_length_1944_cov_3.317628"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLM83_10800"
     CDS             complement(<1..437)
                     /locus_tag="PLM83_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOK71932.1"
                     /translation="MKKSGNVTLIVVLAIVAVLGIGFVSVSNSIVRAEEEVNASWAEV
                     QNQLQRRYDLIPNLVETVKGYAAHEEEVFTAIADARAKLGGAQSVTETAEAVNAMEGA
                     LSRLLVVVENYPELKADASFRDLTYELAGTENRIAVARQRYNTS"
     gene            complement(562..1476)
                     /locus_tag="PLM83_10805"
     CDS             complement(562..1476)
                     /locus_tag="PLM83_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK71933.1"
                     /translation="MVEMIRSSILSATPVLLAGLGGAYTYYADVFNIAMEGMMLIGAF
                     TAVAGSYSSGSWAVGVLSGIAGGLAAALIFAFFVVSLKTDEFVTGTALNMLALGGTTY
                     SLRQLFHVKGAFMSPAIQSIPRWNIPLVSEIPCLGPILSGHPFIVYIGILLAFFVEWH
                     IFNTRFGLRLRAVGEDAIAVDSVGVSSDRIKAQAIMASGVLCGLAGAYLSLGYVTLFA
                     ENMSNGRGWIALAVIILTRGRPMGLLLMSLVFGFFDGLGLSLQGTMIPPQFTQMVPYV
                     VTIVALYCYSREKGTAKGEPEQSKASLS"
     gene            complement(1469..>1944)
                     /locus_tag="PLM83_10810"
     CDS             complement(1469..>1944)
                     /locus_tag="PLM83_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71934.1"
                     /translation="IGYEWRAMGQNPIFSRTVGCRNDAAYVWAMLLSGALAGLAGGLM
                     IMGGMQHRFVKGIGANYAWDGVMIAIVANNNILATGLYALFFSVLQTGAMGMELETSV
                     PSEFSLVLQAIVVLFVVASRESTRMLMNNIAAEMRMKRARGRDKGMGDGRRASNG"
BASE COUNT          491 a          582 c          450 g          421 t
ORIGIN      
        1 gaggtattgt accgctgtct agcaacggct attctgtttt cggtgccggc gagctcgtat
       61 gtgaggtctc tgaaggaagc atccgctttg agctctgggt agttctctac caccacaaga
      121 agcctggaaa gcgccccttc catggcattc acagcctcag cggtttctgt gactgactga
      181 gctccgccca gcttcgccct ggcatcagct attgccgtaa atacctcttc ttcatgtgca
      241 gcataacctt ttaccgtttc cacaagattc ggaataaggt cataacgacg ctgaagctga
      301 ttttggactt cagcccagga tgcgtttacc tcttcctccg ccctgacgat cgagtttgac
      361 acgctcacaa atccgatccc gagcacggcc acgatcgcaa gaactacaat caaggtaaca
      421 ttacctgatt tcttcaatgc aaattccccc cctcatattg atagcaggct cgattaagat
      481 tagcatggaa ttctcggccg cgcaactgcc agccaatttg cgtcttcgag gctgattgcc
      541 tcattaaact ggccctccac gtcatgaaag gctcgccttc gattgctctg gttcaccctt
      601 ggcagtaccc ttctctctcg agtaacaata gagtgccacg atggtcacga catagggaac
      661 catctgagtg aactgtggcg gaatcatcgt tccctgcaag ctcagcccca gtccatcgaa
      721 gaatccaaag accagtgaca tcaggagcag tcccattgga cggcccctgg tcagtattat
      781 gacggcgagg gcaatccaac cacggccatt cgacatgttt tcagcaaaaa gcgtgacata
      841 acctaaggag agatatgcgc ctgcaagtcc gcaaagaact cccgatgcca ttatggcctg
      901 agccttgatc ctgtcggaac tgactcctac ggagtccacc gcgatcgcat cttcgcccac
      961 agccctgagc ctcaatccga accttgtgtt gaatatatgc cattccacaa aaaaggcaag
     1021 gagaataccg atatagacga tgaagggatg cccgctcaaa atcggaccta aacacgggat
     1081 ttcggatacg agtgggatat tccaacgcgg tatcgactga atggccgggg acatgaaagc
     1141 tcccttcacg tgaaagagct gcctgagcga atacgtcgtc ccgcccaatg ccagcatgtt
     1201 tagggcggtc ccggtaacaa actcatcagt tttcaaagat acaacgaaga aggcaaatat
     1261 gagagctgcc gcaaggccgc ctgctatccc agaaagaact cccacggccc atgatccaga
     1321 ggaatagctg ccggcgacgg ccgtaaaggc cccgatcaac atcatgcctt ccatagcgat
     1381 gttgaagaca tcggcgtaat aagtgtaggc gcccccaagg cccgcaagga ggaccggggt
     1441 tgccgaaagg atgctcgacc ttatcatctc aaccattaga tgcacggcgt ccatccccca
     1501 ttcccttatc cctgccgcga gccctcttca tcctcatctc ggcagcgata ttgttcatca
     1561 gcatacgagt cgactctctg ctggccacga caaagagcac tactatcgcc tgaagcacca
     1621 gagaaaactc ggaagggaca gacgtttcaa gctccatgcc cattgcgcct gtctgaagca
     1681 ccgaaaaaaa cagggcgtac agacctgtgg ccagaatgtt gttgttagcc actattgcta
     1741 tcataacccc gtcccacgca tagttagcgc cgatcccttt cacaaaccga tgctgcatcc
     1801 cacccataat catgagcccg cctgcgagcc cagccaaggc tccgctgaga agcatagccc
     1861 atacataggc ggcatcgttt cgacacccga ccgttctcga gaagatcggg ttctgcccca
     1921 tcgctcgcca ttcataaccg atct
//
LOCUS       DAOSAI010000416         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_39263_length_1937_cov_2.924017, whole genome shotgun sequence.
ACCESSION   DAOSAI010000416 DAOSAI010000000
VERSION     DAOSAI010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39263_length_1937_cov_2.924017"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLM83_10815"
     CDS             complement(<1..212)
                     /locus_tag="PLM83_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71935.1"
                     /translation="MSANVNSSTLDSALRNQKNQGPAKASKWGKIIGKVLAYIVVTLG
                     AIMMALPFVWLILSSFKTLEEISLFPP"
     gene            complement(209..1141)
                     /locus_tag="PLM83_10820"
     CDS             complement(209..1141)
                     /locus_tag="PLM83_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOK71936.1"
                     /translation="MSAEATTIPPRNGNERKYKWYRFQKASAPYIFILPGVVFYLMVT
                     IVPMITGFWMSFHNWNIIRPTQPWVGLANYRKLFADPLFWTAMKNTLTYSIGVVPLQM
                     ILALLVALLLNTGIKGKKFFRLLYYLPVITPLSIAAVIWTWIYHPNLGIFNQIVKFIG
                     LAPQDWLGDPAWAMFSVILVAIWSGIGYRMVVYLAALQGIPRSYYEAAEIDGAGNWDA
                     FRYITLPLLKPTTLYILVTSVISSFQVFGLVNVLTGGGPLDATQVVVSYIFKRAFGDL
                     QFGLAAAMSFVLFGIILIITLIQWKFLGKEVTYE"
     gene            complement(1355..>1937)
                     /locus_tag="PLM83_10825"
     CDS             complement(1355..>1937)
                     /locus_tag="PLM83_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOK71937.1"
                     /translation="IEALKFATDMVTTYKVEDPQFGQRYNAFRQDKLGMVFGHPAMIG
                     SFRTTAPNLDFLVAEVPAPKAGGSRSTLLTSWAYVVSSKAESEICSKWVAYLTSADAQ
                     KRWTLTTGELPSRRALLDLPEYKSDKLLAPCMESMKKSVPAPWTTRSLDDELVRKAYQ
                     MVTLKGESPAAALKWLTQEGIKAEKEQQELAKP"
BASE COUNT          465 a          562 c          477 g          433 t
ORIGIN      
        1 ggaggaaaga gcgatatctc ctccaaagtc ttaaaggacg acaatatgag ccacacaaag
       61 ggcagagcca tcatgatggc gcccaaggtc acgacgatat acgcgagcac ttttccgata
      121 atcttccccc acttcgatgc ctttgcaggg ccttgattct tctggttacg caaggcggaa
      181 tcgagggtgc tgctgttgac atttgcgctc attcgtacgt gacctccttt ccaagaaact
      241 tccactgtat cagcgtgatt atgaggatta ttccgaacag aacaaacgac atcgccgccg
      301 caagtccgaa ttggaggtca ccaaaagccc tcttgaatat gtacgacaca acaacttgcg
      361 tcgcatcaag cgggccgcct ccggtcagca cgttcacaag tccgaacacc tggaacgaac
      421 tgattacgct tgtgaccaag atatacaggg tagtgggctt gagtaacgga agcgtgatgt
      481 atctgaaagc gtcccaattc cctgccccgt ctatctctgc tgcctcgtag taactccgag
      541 gtatgccctg caaggccgcg aggtacacaa ccatgcgata cccgattccc gaccatatgg
      601 cgacgagaat caccgagaac atcgcccagg ccggatcgcc caaccaatcc tggggcgcca
      661 gcccgataaa cttgactatc tggttgaata tgccgagatt gggatggtat atccaagtcc
      721 agataaccgc cgcaatcgat agcggagtaa tcacaggcag atagtacaga agcctgaaaa
      781 actttttccc cttaatcccg gtgtttaaca ggagcgcaac aagcagcgcc aggatcatct
      841 gcaagggaac cacgcctatc gaatatgtaa gcgtgttctt catggccgtc caaaacagcg
      901 ggtctgcaaa gagtttgcgg taattggcaa gacccaccca aggctgtgtc ggacggataa
      961 tgttccaatt gtggaaactc atccagaacc ccgttatcat cggcacaatc gtgaccatga
     1021 ggtaaaacac taccccaggc agtatgaaga tatacggggc gctggccttt tgaaagcgat
     1081 accacttgta cttgcgctca ttgccgtttc gtggtggaat tgttgtcgcc tctgcgctca
     1141 cggcgcatca acctccttat ccaagccgag ctgggaccgg acatctggat gcgagatgtg
     1201 cgggaccagg atgccaacac ccggccccgc acatctgcgc gaattccatg tgccacaacc
     1261 tctacagaat cacaaccgga tgtgcgctct cacccgcgtg gggacagtcc cacatagaga
     1321 tgaagttgtg caatacatcc cggtccgcag cttactatgg ctttgccagt tcctgttgct
     1381 ccttttcagc ctttatgccc tcttgcgtta gccacttcag ggcagctgca gggctctcgc
     1441 ccttcagtgt caccatctga taggccttcc ttaccagctc atcatcgaga ctcctggtag
     1501 tccatggcgc gggcactgat ttcttcatcg attccataca aggagccaac agcttgtcgc
     1561 tcttgtattc cggcaggtcg agcagagctc tcctcgaagg cagctcgcca gtggtcaacg
     1621 tccaacgttt ctgcgcatct gccgaagtca ggtatgctac ccacttggag cagatttccg
     1681 actccgcctt gcttgacacc acataagccc acgaagtgag cagcgtggag cgggatccgc
     1741 cggccttcgg tgcagggacc tcagccacca aaaagtcaag attgggcgcc gtggtcctga
     1801 aactgcctat catggctggg tgtccgaaca ccattcccaa cttgtcctgt cggaacgcat
     1861 tgtatctctg gccaaactgg gggtcctcaa ctttgtacgt agtcaccatg tccgtcgcaa
     1921 acttcagcgc ttctatg
//
LOCUS       DAOSAI010000417         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_39790_length_1920_cov_3.051475, whole genome shotgun sequence.
ACCESSION   DAOSAI010000417 DAOSAI010000000
VERSION     DAOSAI010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39790_length_1920_cov_3.051475"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..737
                     /locus_tag="PLM83_10830"
     CDS             255..737
                     /locus_tag="PLM83_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71938.1"
                     /translation="MRVESLTVRICDAEIKHALQNMELPQGLKVVDVKIASGMITLQL
                     ALPYNIKFHPVICLSNPTVQGAVVALRISGPLGLSLPDIVLSSIMPVLPPGVDYLGRG
                     LILIDLLQIYEGAFRSLDLEGISIEPCCITLHVACAEFDLESLPKAASLLQTITIDES
                     "
     gene            complement(789..1604)
                     /locus_tag="PLM83_10835"
     CDS             complement(789..1604)
                     /locus_tag="PLM83_10835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK71939.1"
                     /translation="MRRKARERDDSLCETQYEGFFSEFYDLLHSSYNADLPTYLDLAE
                     EYGDPILELGSGTGRLLIPLARAGYRVTGLDCAEDMFALCRKKLEAENGATRERVTLV
                     NGDMRDFDLDMKFNMVLAACNTILHCTTIDDLLAVLARARRHLSQKGVFVVDFAIPNI
                     LRMLQTNGAEARFEMVHPVSGTRIIDTYVAGYDFTKQMETYEARIEEWDESTMLRWSE
                     VKTERAFYFPREIELALRCSGFEIVKTWKGMRGGPLVETSQDMVYVCRLARQG"
BASE COUNT          477 a          550 c          449 g          444 t
ORIGIN      
        1 atggcatagg ccattccgag gtggcctgct gatggagcaa tcagctaaga tacttcctgc
       61 aggaatggaa taaaggagga agcacacgca gtatcgaaga attctgtaac ctaatgtgaa
      121 agttcggccg gattcgccaa gcaattgcaa gcttcgctcc cagttgcctg aatcgtctgc
      181 tatcgcatac tccccaatcg acgcctataa gaatgagtgg ccgaggggct tctggaaggg
      241 tgggtgagac atgaatgaga gtggaatcct tgacagtgag aatatgcgat gcagaaatca
      301 aacacgccct gcaaaacatg gagctgcctc aagggctgaa ggtagtcgat gtaaaaatcg
      361 catcgggcat gattacgctc cagttggcac tgccgtacaa tataaagttc catccggtaa
      421 tctgcctctc gaacccgacg gtccaagggg ccgtcgtcgc cctcagaatc tccgggccac
      481 taggactcag ccttcccgac atagtcctca gttctatcat gcccgttctg ccgcccggcg
      541 ttgactacct cggacggggg ctcatcctca tcgatctgct ccagatatac gagggcgcct
      601 tccgttcgct cgatctggaa ggcatcagca tcgaaccctg ctgtatcact ctgcatgtcg
      661 cgtgtgcaga attcgatctc gagtctctac ccaaagcggc ttcgttgctc cagacaatca
      721 cgatagacga atcatgaatc taaacatgct tggcctctgg cgcctgtaac atggctagat
      781 cctctcgact aaccctgccg agccaatctg cacacgtaaa ccatgtcttg actggtctcc
      841 accaacggac ctccgcgcat acctttccat gttttgacta tctcaaaacc actgcacctg
      901 agggcaagct ctatctcgcg gggaaaatag aaggcccgtt cagtcttgac ctcgctccat
      961 ctcagcatcg tgctttcgtc ccattcctca atgcgcgcct cgtacgtctc catctgtttg
     1021 gtgaaatcgt agcctgcgac atatgtatcg attatgcgag tgccgctcac aggatggacc
     1081 atttcaaacc tggcctcggc gccatttgtc tgaagcatcc tgaggatatt aggtatggca
     1141 aaatcgacta cgaagactcc tttttggctt agatgccgac gggctctggc aagcacagcc
     1201 agaagatcat caatggtggt gcagtgaagt atcgtattgc acgccgccaa aaccatgttg
     1261 aacttcatgt cgagatcgaa atccctcatg tctccattta caagagtcac tcgctcccgt
     1321 gtagccccgt tttccgcctc aagcttcttc ctgcagagtg cgaacatgtc ttcagcacaa
     1381 tcgagtccgg tcaccctgta tccagccctg gcaagaggaa tcaacagtct cccggttccg
     1441 gatcctagct ctaaaatggg atcgccatat tcctcagcca agtccaagta tgtgggcaga
     1501 tcagcattgt aggatgagtg cagcaagtca tagaactctg aaaagaaccc ctcgtactgc
     1561 gtttcacata gagaatcgtc tcgttctcga gcttttcgac gcatgataag ccaatccccc
     1621 tccaaatatc ataacttgcc tagcgcagac aggaactggc cttgggtttc aacgctcaac
     1681 accaatgaat aaccctggcc agtcagaatg tacacgactt cagctttacc agtcacaaac
     1741 agcaaatcct tctcacgagc gagatcagcc aacctcacat ttcatgcgtc gatgggctct
     1801 cgtaaaacag ccttgccgca tggtcttacg cataagactc tcgcaagaac caattcgaag
     1861 tcgcttagca ggtgggcagg acgaacctcc aaggggcgag cctccgcagt aaccggctgc
//
LOCUS       DAOSAI010000418         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_40520_length_1896_cov_4.049973, whole genome shotgun sequence.
ACCESSION   DAOSAI010000418 DAOSAI010000000
VERSION     DAOSAI010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40520_length_1896_cov_4.049973"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLM83_10840"
     CDS             <1..737
                     /locus_tag="PLM83_10840"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOK71940.1"
                     /translation="LDESCLKCGVHKPTNPTQAARLAREGIPVTPVFNNCSGKHAGML
                     AYATHKGYPIDNYVDKESLVQIDMWKAVAEICGLLPEKVVRGVDGCGVVVFGMPIANM
                     AYAYARLACPDMLPERLREPARRVTSAMVEYPEMIGGTLDFGSNLMKNSKGAVFAKGG
                     AEGLFCVGVPKRGIGIAVKIEDGGSRGLQTAVMETLRLLDVVPQETFAEPQTSIVAFV
                     RNCREDVVGEISPCFELAEGNSRLSV"
     gene            817..1296
                     /gene="tsaE"
                     /locus_tag="PLM83_10845"
     CDS             817..1296
                     /gene="tsaE"
                     /locus_tag="PLM83_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOK71941.1"
                     /translation="MNKGENLKFMSNSPADTAALGCRLGRLLGPGDIVCLEGGLGAGK
                     TCLARGIAKGLGVDETSVSSPTFVLAQEYRGRVRVYHLDLYRLSSSDEVDEAGLDEYI
                     WGDGVAIIEWPGVYVPLREHDRLMVEITQEPVSLRRELVLTPYGQRYRQLLEEMRPC"
     gene            1290..>1896
                     /gene="tsaB"
                     /locus_tag="PLM83_10850"
     CDS             1290..>1896
                     /gene="tsaB"
                     /locus_tag="PLM83_10850"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOK71942.1"
                     /translation="MLILAVDTSTMVGGVALINDGALLGEHVLNVRATHSERLMSSVD
                     ATLTGANIAPGDLDAIACGVGPGSFTGVRIGVSAAKGMSYALGIPLVGVPTLDALAYG
                     RSPGFSEVWSMIDARHGRCYVACYKPSDSFGAAKRILDYSVLDVSQIVDSAGADGDRV
                     LFVGDGALAYSDSLLSAFPGRGVVPPAAFGILRPAQVGMLGM"
BASE COUNT          388 a          427 c          602 g          479 t
ORIGIN      
        1 gtctagatga atcatgcctg aagtgcggag tgcacaagcc taccaacccg acacaggctg
       61 cccgccttgc tagggaagga atccctgtca cgccggtttt caataattgc tcaggcaagc
      121 acgccgggat gcttgcctat gcaacgcaca agggatatcc catcgacaat tacgttgata
      181 aggagagctt ggtccagatc gatatgtgga aggcagttgc tgagatctgc gggcttttgc
      241 ctgaaaaagt tgtgcgcgga gtggatgggt gcggtgttgt ggtctttggg atgcccatcg
      301 ctaacatggc ctacgcctac gcacggctag cctgccccga catgttgcct gagcgtttga
      361 gagagcctgc tcggcgcgtc acttcggcaa tggtggagta cccggagatg atagggggca
      421 cactcgattt cggatctaat cttatgaaga atagcaaagg cgcggtattc gccaagggcg
      481 gcgctgaggg ccttttctgc gtaggtgttc cgaaacgcgg gatagggata gctgttaaaa
      541 tcgaagacgg aggatccaga gggttgcaga ctgcagttat ggagactctg cgcctattgg
      601 atgtggttcc ccaagagacg ttcgctgagc cgcagacatc tattgtggcg tttgtccgca
      661 attgccggga agacgttgtt ggggagatct ctccatgctt tgagctcgca gaaggcaatt
      721 ctcgtttgtc ggtttgacct tggccggggg agtggagtat aatcttgcgg ctaatagggt
      781 cagcacaggc acattgcgag gttatgcgga ttgagcatga ataagggtga aaacttgaag
      841 tttatgtcga attcgcctgc tgataccgcc gctttaggat gcaggctcgg cagactgctg
      901 gggcctgggg atattgtttg cttggaaggc ggtctgggtg caggcaagac ttgtttggcg
      961 cgagggattg ccaagggatt gggtgttgac gaaacatctg tatcgagccc aactttcgtg
     1021 cttgcacagg aataccgggg cagggttaga gtatatcatc tagaccttta ccggctgtcc
     1081 tcaagcgatg aagtggacga agcaggcctt gatgaataca tctggggcga cggagtcgcc
     1141 atcatcgaat ggcctggagt ctatgttcca ttgagggagc atgacaggct tatggtggag
     1201 attactcagg agccggtcag tctcaggcgg gagcttgtgt tgactccata tgggcagaga
     1261 tatcgacaat tgctggagga gatgaggccg tgctgatcct cgctgtagat acgtctacca
     1321 tggttggggg cgtcgctctc atcaatgatg gagccctctt aggagaacat gttctgaacg
     1381 taagagctac tcattcggag aggcttatgt cgtcagtaga tgccacgctt acaggcgcca
     1441 acattgctcc cggtgatttg gatgccatag catgcggtgt agggcctggt tcgtttacag
     1501 gagtccgcat aggggtgtct gcagccaaag gcatgtcgta cgccctcgga attccgttgg
     1561 tgggagtgcc gactcttgat gcccttgctt acgggcgtag ccccgggttt tctgaagtgt
     1621 ggtcgatgat agatgctcgc catgggcgtt gctacgtggc ctgttacaag ccttctgata
     1681 gcttcggcgc tgcaaaacgg atattggact attctgtcct tgatgtttct cagatagttg
     1741 attcagcagg cgctgacggg gatcgtgttc ttttcgttgg cgatggtgcc cttgcgtatt
     1801 ccgactcttt gctttcggct tttcctggac gcggggttgt gccgccagcg gcgttcggga
     1861 ttctcagacc ggcccaggta ggaatgttag gcatgg
//
LOCUS       DAOSAI010000419         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_41835_length_1856_cov_3.394225, whole genome shotgun sequence.
ACCESSION   DAOSAI010000419 DAOSAI010000000
VERSION     DAOSAI010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41835_length_1856_cov_3.394225"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLM83_10855"
     CDS             <1..180
                     /locus_tag="PLM83_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010495545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HOK71943.1"
                     /translation="GGSGCACSALITYGFILKRMCQNEISRALVVATGALMSPISWQQ
                     GESIPCIANAVVLET"
     gene            241..591
                     /gene="spoVAE"
                     /locus_tag="PLM83_10860"
     CDS             241..591
                     /gene="spoVAE"
                     /locus_tag="PLM83_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HOK71944.1"
                     /translation="MDYLVAFLVGGTICAIGQVLLDTSKLSPPHMLVAFVVAGAILSG
                     LGVYDKLVDFAGAGALIPVSGFGHSIARGSLMEAERLGVLGLFTGALEFTGLGITTAV
                     FFGALIALVANPKP"
     gene            594..1583
                     /locus_tag="PLM83_10865"
     CDS             594..1583
                     /locus_tag="PLM83_10865"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOK71945.1"
                     /translation="MRVGVPRGLLYHEYAPAIHTFLESCGAETVVSPQTNREILEKGL
                     ALCVDDACLPVKAFFGHAAVLSDQVDALFVARFVSVERGAYICPKFMGLPDMIRARLQ
                     NAPPIIDMCVDIRQGLGSYVSSLPCSDTCKILGVAPRHLSWALGAALMAQRKEDRRRV
                     KRFAPSGSAVARTGLGGYTRESIYETSLTIGVLGHTYNLDDAYLSLGLISKLEKMGVK
                     AVTAEDYAYDNLRRWERRGADRKQVFWTAGRKLIGAARRWRETGEVDGMVHLTSFGCG
                     PESFVAEVIARDAARHPAMPLIILNVDEHSGEAGMATRLEAFVDTVRMGKSKR"
     gene            1580..>1856
                     /locus_tag="PLM83_10870"
     CDS             1580..>1856
                     /locus_tag="PLM83_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOK71946.1"
                     /translation="MKVSFPHLGNLWIPLKAFFSGIGIEVVAPPPTTRKTVAIGAQLA
                     PETACLPLKVTIGNMIEAINSGADTFLMVGGIGPCRLGYYCELQRDIL"
BASE COUNT          384 a          476 c          560 g          436 t
ORIGIN      
        1 gggggcagcg ggtgtgcgtg ttcagctctg atcacctacg ggttcatctt gaagaggatg
       61 tgccagaacg agatatctcg ggccctcgtc gttgccacag gcgccctcat gtcgcccatc
      121 tcctggcagc agggagaatc gattccttgt attgcaaatg ctgtcgtgct cgagacgtaa
      181 tcgccatgtt caggtcatca aggcccatat attgtagcct tagggaggaa ctttggtcac
      241 atggactacc tcgtggcgtt tcttgtagga ggaacgatct gtgcaatagg ccaggtgctg
      301 ctcgacacga gcaagctttc acccccgcac atgctagtag cctttgttgt agccggcgca
      361 atcctctcag gtctgggagt ctacgataaa ctggtggatt ttgctggggc aggcgctcta
      421 attcccgtat caggctttgg tcattcaata gcccgcggct cgctcatgga ggcggaaagg
      481 ctcggcgtac tggggttgtt caccggtgct ctggagttta cagggcttgg gataaccacg
      541 gcggtttttt tcggcgcatt gattgctctc gttgccaatc ccaagccatg accatgagag
      601 tgggagttcc ccgcgggctc ttgtaccacg agtatgcgcc tgccatccac acttttttgg
      661 agtcttgcgg agccgaaaca gtcgtgtcgc cgcaaaccaa ccgggagata cttgagaagg
      721 ggcttgctct gtgtgtagac gatgcctgtc tgcctgtaaa ggcttttttc gggcatgccg
      781 ccgttctgtc ggatcaggtt gacgccttgt ttgttgcgag gtttgtgagc gtggagcgtg
      841 gagcgtacat atgtccgaag ttcatgggcc tccccgacat gatcagagcg aggctgcaaa
      901 atgcgccgcc tatcatcgat atgtgtgtcg acatccgcca gggcctcggt tcgtatgttt
      961 catccctgcc ttgctcagac acttgcaaga ttctaggagt ggcaccacgg catctatcat
     1021 gggcattggg cgccgccttg atggcgcagc gaaaggaaga ccgtcggcgc gtcaagaggt
     1081 ttgcgccctc aggctctgcc gtagcccgta cggggctagg cggatacacg cgtgaaagca
     1141 tctatgaaac ctcgttgacc ataggggtgt taggtcatac ctacaacctc gatgacgctt
     1201 atcttagctt gggcctaata tcaaagcttg agaagatggg cgtgaaggca gtcacagcag
     1261 aggattacgc atacgacaat ctgaggcggt gggagaggcg gggggcggac agaaaacagg
     1321 tcttctggac tgccggccgc aaactgatcg gggcagctcg cagatggaga gagacgggag
     1381 aagtggacgg aatggtgcac ttgacctcct ttggttgcgg gcctgagtca tttgttgctg
     1441 aagtaatcgc ccgagacgcg gcgcgccatc ccgccatgcc tctaatcatc ctcaatgtgg
     1501 atgaacactc aggcgaagcc ggcatggcaa cgcgtctgga agcgttcgtg gacactgtca
     1561 gaatggggaa gtcgaagcga tgaaagtctc ctttccgcat ctgggaaact tgtggattcc
     1621 cctgaaagct ttcttctcag gaatcggcat tgaggtggtt gcgccgcctc ccactactcg
     1681 aaagacagta gcgataggcg cgcaacttgc ccccgaaaca gcgtgccttc ctctcaaggt
     1741 aactattggg aacatgattg aagcgatcaa cagcggcgcc gacacttttc tcatggtggg
     1801 tgggataggt ccgtgtaggc ttggctacta ctgcgagctg cagcgagaca tactct
//
LOCUS       DAOSAI010000420         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_42265_length_1842_cov_3.203134, whole genome shotgun sequence.
ACCESSION   DAOSAI010000420 DAOSAI010000000
VERSION     DAOSAI010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42265_length_1842_cov_3.203134"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLM83_10875"
     CDS             complement(<1..510)
                     /locus_tag="PLM83_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71947.1"
                     /translation="MNVRDEPANRICEVEDEYAFLGCNALRGDGYTAVLSDICPDMRD
                     SNHVRQINLPRAADPEGFISRAEELFRSKGREHCRFDLDCRTRPQCLYTALRKSGYVP
                     HPTVVQVYRGHDAEGAGGVFSGLSGGERDDRQSDSHPSASVTEVLPATGSEEWRNLMR
                     EHYAGYGQCG"
     gene            complement(565..>1842)
                     /gene="lysA"
                     /locus_tag="PLM83_10880"
     CDS             complement(565..>1842)
                     /gene="lysA"
                     /locus_tag="PLM83_10880"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOK71948.1"
                     /translation="AVRNACRAHRREFESRYPRVRILYASKALMTKAIAMIVAEEGLS
                     LDVCSGGELYTALSAGCPPDRIYFHGNSKTLSEIDFALSAGICRFVVDNMDELRLIDL
                     LANAKGLVADVILRLTPGIEAHTHEYIKTGQIDSKFGIAIPNGDALRAVKYAKELRNV
                     KLTGLHCHVGSQIFETEPFLHAIEVMMDFAREACDATGFIMEELDIGGGLGVRYTGGD
                     DPLSVADLAEAIAPALLKAAEERCLPLPTLLMEPGRAIVGEAGTTLYTVNAVKHIEGV
                     RTYVTVDGGMADNPRVALYQAEYDAVLANKADRPPTCEVSIAGNCCESGDMLAWDIAL
                     PDVEVGDILAIFCTGAYNYSMASNYNRHLRPAMALVKDGWSDLIVERETYEDLVRKDV
                     VPDRLKSSHRSAAVSAESAMLVYPRASGANASE"
BASE COUNT          412 a          577 c          440 g          413 t
ORIGIN      
        1 accgcactgg ccataccctg catagtgctc ccgcatcaag ttcctccact cttcagaccc
       61 ggtcgcaggt aaaacttcgg ttacgctcgc agaagggtga gaatctgact gcctgtcgtc
      121 tctttcaccg ccactcaatc cactgaaaac tccgcccgca ccctctgcat catggccgcg
      181 ataaacctga acgactgttg gatgcggaac atagccagac ttcctcagcg ccgtgtaaag
      241 gcactgaggc cgtgtacggc aatccagatc aaacctgcaa tgctcgcgcc ctttgctcct
      301 gaacaactcc tccgccctgc tgatgaagcc ctcaggatcc gctgcccgag gcaaattgat
      361 ctgcctgaca tggttcgaat ctcgcatgtc ggggcatata tcggaaagga cggctgtata
      421 cccgtctccc cgcaatgcgt tgcatccgag gaatgcatat tcatcctcca cttcacagat
      481 ccggttggca ggttcatcac ggacgttcaa aaccttaacc cccatgtcaa cccggatcgg
      541 ccgagcctcg ccaacattct cccattattc agacgcgttc gcccccgaag ccctaggata
      601 gacgagcata gccgactctg cgctcactgc cgccgaccta tgtgatgact tgagccgatc
      661 tggcaccacg tccttgcgga cgagatcttc gtatgtctcg cgctccacga tcaagtccga
      721 ccagccatcc ttcaccagcg ccatagcggg acggagatgt cgattatagt tgctggccat
      781 ggagtagttg taagcgccgg tgcaaaatat agccagtata tcgccgactt ccacatccgg
      841 tagcgcaata tcccatgcca acatgtcgcc tgactcgcaa caatttcccg caatcgacac
      901 ctcgcatgtg ggaggccgat cggccttgtt tgccaggact gcatcgtatt ctgcctggta
      961 aagtgcaacc cggggattgt cggccattcc tccgtctaca gttacatatg tccgcacgcc
     1021 ttctatgtgc ttgaccgcat ttaccgtgta aagagtagtc ccggcttcgc ctacgatggc
     1081 ccttcctggt tccatcagca atgtgggaag aggcaagcac cgctcttcag ccgccttgag
     1141 caacgcgggc gcaatcgcct cggcgagatc cgccacgctc agaggatcat caccgcctgt
     1201 gtaccgtact cctaggccgc ctccgatatc cagttcttcc atgataaagc ccgttgcgtc
     1261 acaagcttcc cgcgcaaaat ccatcattac ctctatcgcg tggagaaaag gctcagtctc
     1321 gaatatttga gagcccacgt ggcagtggag ccccgtcagc ttcacgttcc tcagttcctt
     1381 agcgtattta actgcccgaa gagcgtcgcc attgggtatg gcgattccaa acttcgaatc
     1441 tatctgcccg gtctttatgt attcgtgagt gtgcgcctcg attcccggag tcaatctgag
     1501 gatcacatcg gccacaagcc ctttggcatt cgccaacagg tctatgaggc ggagttcatc
     1561 catgttgtca acaacaaaac ggcaaatccc tgcagaaagg gcgaaatcga tctcgctcag
     1621 tgtcttgctg ttgccgtgga agtatatgcg gtcaggcgga caaccggcag aaagagctgt
     1681 atacaattcg ccgccggaac agacatcgag agacaatccc tcttcggcaa ctatcatcgc
     1741 aatagccttc gtcatcagtg ctttgcttgc atacaatatc cgaacccgtg gatacctgga
     1801 ctcgaactca cggcgatgag cacggcaagc gttgcgcacc gc
//
LOCUS       DAOSAI010000421         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_44393_length_1779_cov_3.597448, whole genome shotgun sequence.
ACCESSION   DAOSAI010000421 DAOSAI010000000
VERSION     DAOSAI010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44393_length_1779_cov_3.597448"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PLM83_10885"
     CDS             complement(<1..799)
                     /locus_tag="PLM83_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOK71949.1"
                     /translation="MSQLVVVDGDIVDVEEAHVSVLDRANLFGDGLYEVIRCYSGCLF
                     ELATHVSRILEGAKALGILHDWTSESVELAARRLQAETGIMEGEMYIQLSRGPAVRTH
                     YFPKIQSPTMFITLSPVRPIPEGARTVGTSVITCPDMRHGWCHLKTVNLLSNCLAKEK
                     AARHGVWEGLMLRGLDRSALPRTVEEYCDVPGELSGARPGDDVAGWVTEGASANLFVV
                     KDRRIATPPACNILPGVTRRIALELAELAGLDAEERPVAAKELLTADE"
     gene            990..>1779
                     /locus_tag="PLM83_10890"
     CDS             990..>1779
                     /locus_tag="PLM83_10890"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOK71950.1"
                     /translation="MIRVPKTKDGARVGITDTTFRDAHQSLFATRMRTEHMLELAAYL
                     DRVGFYSMEVWGGATFDTCLRYLDEDPWERLRLLRQALVNTPMQMLLRGQNLVGYRPY
                     PDDVVDAFVNKMVELGIDIVRIFDALNDVRNIERAALAVKNAGAHFQGTISYTISPVH
                     TIDGYVKYAQQLKDIGADSICIKDMAGILAPYAAEELVGKLVETVGLPIHVHCHYTSG
                     MASMTYLKAIEAGATVVDCSTSTVALGSSQPPSESMIVALAGSDH"
BASE COUNT          417 a          548 c          451 g          363 t
ORIGIN      
        1 cctcatccgc tgtgagaagc tcctttgccg caacgggcct ctcctctgcg tcaagcccgg
       61 ccaattccgc aagctccaac gcgattcggc gcgtaacccc aggcaaaatg ttgcaggcag
      121 ggggagtagc gatccgccgg tccttcacaa cgaacagatt ggcgctggcg ccttcagtaa
      181 cccaccccgc aacatcatcc cccggccttg ccccggacag ttccccaggc acatcgcagt
      241 attcctccac agtccgaggc aacgcgctcc ggtccagccc cctcagcatg agcccctccc
      301 acaccccatg ccttgcggcc ttttctttgg caagacagtt cgacaacaga ttcaccgtct
      361 tcaaatggca ccagccatgt cgcatgtccg ggcaggtaat gacggaagtc ccgactgtgc
      421 gcgctccttc cgggatgggc ctgacaggcg aaagcgtgat aaacatggtt ggcgactgta
      481 tcttggggaa gtagtgcgtc cgaaccgccg ggcccctcga aagctggatg tacatctcgc
      541 cctccataat ccccgtctcg gcctgcagcc gcctggccgc caattccacc gactccgatg
      601 tccaatcatg caagatgccg agcgccttcg cgccttcaag aatgcgtgac acgtgggttg
      661 ccaactcgaa caagcatccc gagtagcatc gaatgacttc gtatagtccg tccccgaaga
      721 gattggccct gtcaagcacg ctcacgtgcg cctcctcaac atcgacgata tccccatcta
      781 caacgaccag ctgtgacatc cctacttcac ctccgcacta cagagatctt cgccacccaa
      841 catcatccat cctccaaccg ggatgtccca caagcggatc tgctaaaacc tccctaggtt
      901 gctgcccatt tgacagaaga ggtgctatac tatgtgttaa gcgcgtcaga ggacgccgat
      961 taatctcgcc cgaaaggggg atggagaaga tgatcagggt cccgaaaaca aaagacggcg
     1021 ctagagtggg gataaccgac acaacatttc gcgacgccca tcagtccctt ttcgccactc
     1081 ggatgcgcac cgaacacatg ctcgagctcg cagcctattt ggatcgagtc ggcttctact
     1141 ccatggaagt ttggggaggg gcaacattcg acacgtgtct gcgctatcta gatgaagatc
     1201 catgggaaag gttgcgcctt ttgaggcaag cgcttgtgaa cacgcccatg cagatgttgc
     1261 ttcgcggcca gaacctcgtc ggttaccggc cttaccccga cgatgtggtc gatgcattcg
     1321 tcaacaaaat ggtggagctg ggaatagata ttgtaaggat attcgatgcc ttaaatgatg
     1381 tgagaaatat agaacgcgcg gcccttgccg taaagaatgc aggcgcccat ttccagggaa
     1441 cgatatccta caccatcagc cctgtgcaca ccatagatgg gtacgtcaaa tacgctcagc
     1501 agctcaagga catcggcgca gattccatat gcataaagga catggcagga atcctcgcgc
     1561 cttacgccgc tgaagaactc gtggggaaac tggttgagac agtaggcctg cccattcatg
     1621 ttcattgcca ctatacgtca ggaatggcat ccatgacgta tctcaaggcg attgaagcag
     1681 gagcaacagt agttgactgt tccacctcca cagttgcgtt gggttcgtcg cagccgccat
     1741 cagagtccat gatagtagct ctcgcaggct cggatcacg
//
LOCUS       DAOSAI010000422         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_44698_length_1771_cov_3.289044, whole genome shotgun sequence.
ACCESSION   DAOSAI010000422 DAOSAI010000000
VERSION     DAOSAI010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44698_length_1771_cov_3.289044"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLM83_10895"
     CDS             <1..949
                     /locus_tag="PLM83_10895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK71951.1"
                     /translation="WRLYEDINRKLAEAFPGGPWDIVVIHDPQPLAVLTFLDLISESD
                     RPVVGRWFWRCHIDLSTPLEETWQKLWPYVNAYDGAIVTSRQYARDEMTLPVAEITPS
                     TDPTSPKNLPIPEGEARKMMGSFGVDPARPIIVQVSRFDPWKDPFGVVEAYRMLKGKW
                     PELQLAMVGSIAADDPEGIGILAHLKEEAAGDRNIHVLSNEDGVGAREVAAFQQCADV
                     VIQKSIREGFGLTITEAMWKGRPVVAGNATGCKIQITDGVDGFIVGTTQQCAERIDEI
                     LSDPDIGARLGGAARETVRRRFLSTQHVANYLNLFAGCQ"
BASE COUNT          409 a          427 c          499 g          436 t
ORIGIN      
        1 gtggcgactg tacgaggaca taaacaggaa gttggccgag gcattccccg gaggcccttg
       61 ggacatagtc gtgatccatg atccccaacc cttggctgtg ttgaccttcc tggatttgat
      121 ttccgaatca gatcgccccg ttgttggcag gtggttttgg cgatgccaca tcgacctgtc
      181 aacgccattg gaagagacgt ggcagaagct ctggccatac gtgaatgcat atgatggagc
      241 gatcgttacg agccgacaat acgcgcggga cgagatgacg cttccggttg ctgagataac
      301 tccgtccacg gatcccacta gcccaaagaa tctccctatt ccagagggcg aagcaaggaa
      361 aatgatgggc tcatttggcg tagatcctgc tagacccatc attgtgcagg tatcaaggtt
      421 tgatccttgg aaggatccgt ttggcgtggt ggaagcgtat cgaatgctca agggaaaatg
      481 gcctgagctc caacttgcaa tggtcggttc tatcgctgcc gacgaccccg aaggcatcgg
      541 gatacttgca cacttgaaag aggaggcggc tggggacagg aacatacatg tgctgtcgaa
      601 tgaggacggt gtcggcgcgc gggaggtggc tgcattccag caatgcgcag atgtggtcat
      661 acaaaagtct atccgcgagg ggtttggcct tacgataact gaggctatgt ggaagggacg
      721 acctgttgtc gcaggcaatg ccactggatg caagattcag ataaccgacg gcgtggatgg
      781 ttttatagtt gggactactc agcagtgcgc tgaaaggatt gatgagatac tgtccgatcc
      841 ggacattggg gccaggcttg gtggcgcggc tcgggagaca gtgaggcgaa ggtttctttc
      901 aacgcagcac gttgcgaact acctgaacct tttcgctggt tgccaataac cggccacatg
      961 ttttggtcag aaaccgttgt ttaattgcgt atgctgctcc agggtgatga tgttgccctg
     1021 gagctttgct ctatggtgtc ctggcatttc actcttgggg gacgggcgat tttgagaagt
     1081 cgtacgaatt tggcgatggt cgatgctgat tttcggacgg gatctgccag tttgctcatc
     1141 aagtctagat aggctcttgc gcgatcctcg agtttgcgtt gtctacaatt cggacctcag
     1201 cctctcataa gcttcttctt gtgagttatc cagagctatc ggctcggtga tggttatacg
     1261 gccaatagtc tggacaaata ggaatcatca gcgtaggttc cccttgtcaa tatgttggat
     1321 tagaaatccg cagataggca taacccatgt agctggtgcc aagtgttgaa gcgtcgcaaa
     1381 gcctttctag cgattgtttg tttcagctgt gggtgaccta ccattgcgca attcaggcga
     1441 cccggtatcc ataattcttt catcaagctg actcactggt gggcacctat actctgaggt
     1501 aacccggtcc gagtaacttg gcgcgtaaat ccccgaagtc cacgtccgcg cgcatcatca
     1561 tcttgcacac gagaggctga atggcctgac acgaccaata gccgcaaaca ccgcaaagac
     1621 aactacgaac tgcattgaca atggccagca ctgattgggc agttcaaatg gtgaagatgt
     1681 atgcctgcct cacctaggtt atttactgca accccaaaag atattcttgg cccttgccag
     1741 ccagataacc cgagctcgtc acgatgtgat t
//
LOCUS       DAOSAI010000423         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_44805_length_1768_cov_3.113252, whole genome shotgun sequence.
ACCESSION   DAOSAI010000423 DAOSAI010000000
VERSION     DAOSAI010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44805_length_1768_cov_3.113252"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..>1768
                     /locus_tag="PLM83_10900"
     CDS             121..>1768
                     /locus_tag="PLM83_10900"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOK71952.1"
                     /translation="MVHGQNEPRTLEELLAKAGANRPDADLSVIERAWDYARDAHDGQ
                     VRESGEPYFDHPVAVAFILADMNLDPNTIAAGLLHDVIEDTGRSYEEISQVFGSEIAT
                     LVDGVTKLSRMDFKSREEQQAESLRKMFVAMAKDIRVILIKLADRLHNMQTLDFSPPD
                     KQKRVAGETLEIYAPLAHRLGMWRMKWELEDLSLRYLDTDAYYDLVEKVATKRQEREG
                     YIEEARETLEAALTEIGISAEIQGRPKHFYSIYRKMEKQGKDFSEIWDLMGLRVIVGT
                     LRECYAVLGAVHSIWKPIPGRFKDYVAMPKSNMYQSLHTTVIGPRGEPLEIQIRTWEM
                     HRTAEYGIAAHWRYKEGGGGGDFEDKITWLRQILEWQKETRNGTEFVETLKVDLFSDE
                     VYVFTPRGDVKTLPAGSTPIDFAYSVHTDVGHSCSGARVNGRMVPLNTKLATGDIVEI
                     ITSKSQAGPSRDWLAIAKTSKAKGKIRQWLKEEQREASVEQGKELVEKELKKLGLQVR
                     ANMKEERLLEVAQKFSYQSTDDLLASVGYGKLSAAQVVTKL"
BASE COUNT          451 a          412 c          555 g          350 t
ORIGIN      
        1 atcgtgaaag acctgagaaa gtcccaggat gcataatggc gtcgcagttt gtggccgcgc
       61 ttgcagtgcc gtagtctaag aaagaatcct ctccgaggtt ttgaaagggg ggcggcgggg
      121 atggtccatg gtcagaatga gccgcgcaca ttggaagaat tgcttgccaa ggccggagca
      181 aaccgtccgg atgctgatct tagcgtcata gagcgcgcgt gggattatgc tcgtgacgcc
      241 cacgacgggc aggtgcgcga gtctggcgag ccatactttg accacccggt cgcagtggcg
      301 ttcatcctcg ctgacatgaa tttggatccc aacaccatag ctgcgggtct gctccatgac
      361 gtgatagagg acacgggaag gagctacgag gagattagcc aggtgtttgg ttcggagatt
      421 gctacgcttg tggacggggt aaccaaattg tcgcggatgg atttcaagag tcgcgaggaa
      481 cagcaggctg agagcctgag gaagatgttc gtggccatgg ccaaggacat tcgggtcatc
      541 ctgatcaaac tggcggacag gctccacaac atgcagactt tggatttctc ccctcctgac
      601 aagcagaaac gggtggccgg tgagacgctg gagatatatg ctcctttagc gcatcggctt
      661 ggaatgtgga ggatgaaatg ggaactggag gacctatccc tcaggtatct tgataccgat
      721 gcatactacg atctggtaga gaaggtcgct acaaagaggc aggagaggga aggatacatt
      781 gaagaggctc gcgagacgct cgaggctgcg ctcacagaga tcgggatttc cgctgagatc
      841 cagggacgtc cgaaacactt ctacagtata taccggaaga tggaaaagca aggcaaggat
      901 ttcagcgaaa tctgggatct catggggctt cgggtaatag taggcacact tcgtgaatgt
      961 tatgcggtgc ttggagcagt ccacagcatc tggaagccaa ttcccggacg tttcaaggac
     1021 tacgttgcca tgcccaagtc gaatatgtat caatccctcc atacgactgt tatcggccct
     1081 aggggcgagc ctctggagat ccagatacgc acgtgggaga tgcacagaac tgccgaatac
     1141 ggaatcgctg cccactggcg ctataaggag ggcgggggag ggggcgattt cgaagataag
     1201 atcacctggc tcaggcagat cctcgagtgg cagaaagaga cgagaaacgg cacggagttc
     1261 gttgagacgc tcaaggtcga cctcttctcg gacgaggtat acgtctttac cccgcgcggt
     1321 gatgtgaaga cgctgcctgc tgggtcaacc ccgatcgact ttgcatattc cgtccacact
     1381 gatgtgggac actcatgctc gggagcgcga gtgaacggga ggatggttcc gctcaacact
     1441 aagctggcaa ctggcgacat tgtggagatc attaccagca agtcgcaagc aggtcccagt
     1501 agagactggc tggccatagc caaaacatcg aaagccaagg gaaagatcag gcaatggctt
     1561 aaggaagagc aacgagaagc atcggtggaa cagggcaagg agcttgtgga gaaggaactg
     1621 aaaaagctcg ggctccaagt gcgtgcgaac atgaaggaag agcgattgct cgaggtggcg
     1681 cagaagttca gttatcagag taccgatgac ctgcttgcaa gtgtaggtta tggcaagctg
     1741 tcggcggccc aagttgtcac caaactcg
//
LOCUS       DAOSAI010000424         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_45595_length_1747_cov_2.717494, whole genome shotgun sequence.
ACCESSION   DAOSAI010000424 DAOSAI010000000
VERSION     DAOSAI010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45595_length_1747_cov_2.717494"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLM83_10905"
     CDS             complement(<1..201)
                     /locus_tag="PLM83_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71953.1"
                     /translation="MLNHFVEITEAVREPSLSPVPVRRLDFDQVIISIFGLRLEADRR
                     TLLFYYFAFLPSSVRSGVVELLV"
     gene            complement(230..1078)
                     /locus_tag="PLM83_10910"
     CDS             complement(230..1078)
                     /locus_tag="PLM83_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71954.1"
                     /translation="MQIRSEILDTYLRGESTERFSEHWIFQTVDRFLPQGQSAYTPPD
                     ESEEARAAIRRVAQEIREAVDGFVPNNVEVWDALFSGWRLIIDATKINMVVGLPDIYD
                     ALALSDPSGAIQVVYDLPNWLRYEGKHNISDVVGNLLTHELCHVCIGAIYTDIETDSE
                     SADHIMCIDAMAFNEGFAHLVSYNYTPIDKVDWESETLRNVYKKSIAQLKLALAETDP
                     PRQQQYLLDAVCGSYYDKYACMAGMLFLAKRWREGGIPALVDEFERGYHGFAQNIVDN
                     TKVSVE"
BASE COUNT          409 a          455 c          440 g          443 t
ORIGIN      
        1 taccagcaac tccacgacgc ctgaacgaac gctagagggg agaaaagcaa aatagtagaa
       61 aagcagcgtt ctcctgtcag cttccagccg aagcccgaag atgctgatga taacctgatc
      121 gaagtcgagc ctgcgtacag gaacgggaga taatgatggt tctcttaccg cttctgtgat
      181 ttcgacgaaa tggttgagca accaacaccc aagcagccag agagcgtgat tattcgacgc
      241 tcacctttgt attgtccacg atgttttgtg cgaacccgtg gtagccgcgt tcaaattcat
      301 cgacaagcgc aggaatgccg ccctctctcc atcttttagc gaggaacaac atgcctgcca
      361 tgcacgcgta tttgtcataa tagctgccgc acacggcatc aagcagatat tgctgctgcc
      421 ttggcggatc cgtttctgcc agagctagtt tcagctgcgc aatgcttttc ttgtatacat
      481 tcctgagggt ttcagactcc caatccactt tatcgatggg cgtgtaatta taggaaacca
      541 aatgggcgaa cccctcattg aatgccattg cgtctatgca catgatatga tcagcagatt
      601 ccgaatcggt ttcaatatct gtgtagatgg caccgataca tacatggcac agttcatgtg
      661 tgagcaggtt gccgactaca tcagatatgt tgtgctttcc ctcgtatcgc agccagttgg
      721 gaagatcata gaccacttga atggcgccgg aaggatccga aagagccaat gcatcatata
      781 tgtcggggag gcctacaacc atgttgatct tggttgcgtc aatgatgagc ctccagccgg
      841 aaaacaaagc atcccatact tccacgttat tgggaacaaa cccgtcaaca gcctctctga
      901 tctcctgcgc aacgcggcga atcgccgcac gggcctcctc actctcatca ggtggggtat
      961 atgcggactg gccctggggg agaaatctgt cgaccgtttg gaatatccag tgttcgctga
     1021 acctctcagt ggattctccg cgaaggtatg tgtcgagtat ttccgaccta atctgcaaat
     1081 agaggacctc ctatccgcat cggttcttcg tcggatagta tttaatttat cgcattcgcc
     1141 atttgccaca caattccttg cccactaccg tgttcgatcg gtcctcagat cgtgtgcaag
     1201 gtaagctatg gggaggcttg cctagtccgc ctatcgcagc tatcctttgg ctcacatttc
     1261 gccttcaaat tgatgctgct caatactcgg ccaaacagtg tgccgtgggg cccggagctg
     1321 aagaagaggc aagcttcgtg ggcctactat agagattccg cggttggccg atgaccgctg
     1381 tcgtgctaat gccagacgcc ttgggcgctg aatacgcacg taggttatat ccctgtcaag
     1441 tgctcaataa ctgggatttc aactgccttc atcctcaatt ctgtaggctt tagttcgcgc
     1501 aagtatactc aaatgaggat tttgtctccg gtcgctgcga atggcttgag acctaacagg
     1561 cttcaaccag gctttggaga acatcgtcgc gcgtggtctc tagcgactct gatctgctat
     1621 cgatcatgtg aagaggaggg cgtcgggatt catggaacct acagtccctt ctggcgcaaa
     1681 gcttgacagg gcgaaggggt ttggggctgc aacattctct tgtcccgcgt gctgatcgaa
     1741 tctgtcc
//
LOCUS       DAOSAI010000425         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_45867_length_1739_cov_3.151425, whole genome shotgun sequence.
ACCESSION   DAOSAI010000425 DAOSAI010000000
VERSION     DAOSAI010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45867_length_1739_cov_3.151425"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PLM83_10915"
     CDS             complement(<1..1273)
                     /locus_tag="PLM83_10915"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOK71955.1"
                     /translation="MRTKAKGVLLIVVVVALCAPRICSANMYPGSECHGTPDFSVDLN
                     NIDGIIRELASEKYMGRLPGTQGNWDAVEYVADYFRRLGLVCPESLDGYLQPYVQPVR
                     MFRRAPELQIVDAVGNLVSNFNYVEQYAPAPMQGWMVSKEVVGEGVVLERLSHLDDME
                     DGKILLIPLPISESAADMHILYNRLASEDEDPPEFAGIVLETDIAEIGYFPVYGLISQ
                     DVEFQDRDAHLSSGPVIFKCDVDVFSRLVEASGCGLRIRMDWDFAVEDVIAANVVAVL
                     LGPDGEVDEDHVIIGAHIDHMGSNFNGTYNPGAMDNASGMAAMMEIARLLAETAHRPR
                     RAVVFIAFNGEEEGMWGSRYYSNNPCYPLDEAVMINIDNVGSKYDVPLVIEEYAMMLT
                     SLVYHLYGIALQHGIDSEMGSGSMCDNASFAL"
BASE COUNT          419 a          505 c          413 g          402 t
ORIGIN      
        1 caagcgcaaa cgaggcgttg tcgcacattg aaccgcttcc catctctgag tcgattccgt
       61 gctgcagggc tatgccataa agatggtaga caagactagt gagcatcatg gcgtattcct
      121 cgatgactag gggaacatcg tatttcgatc cgacattgtc gatgtttatc atcactgctt
      181 catccagagg atagcacggg ttgttcgagt aatacctcga tccccacatc ccctcttcct
      241 ccccgttgaa ggcaatgaag accaccgccc gcctcgggcg atgcgcagtt tccgcaagca
      301 gcctggctat ttccatcata gccgccatcc ctgaggcatt gtccatggcc cccgggttat
      361 acgtgccgtt gaagttgctg cccatatggt cgatatgggc accgatgata acgtggtctt
      421 cgtccacctc tccatcaggc cccaaaagca cagcaacgac attagcggca ataacatcct
      481 ccaccgcgaa atcccaatcc atcctgatgc gcagcccaca tccgctcgct tcaaccagcc
      541 gcgagaagac gtcaacatcg cacttgaaga tcacaggtcc cgacgagaga tgggcatcac
      601 ggtcctgaaa ctcgacgtcc tgcgatatca gaccgtatac ggggaaataa ccgatctcag
      661 caatgtctgt ctccaggaca atgccggcaa attccggcgg atcttcatct tctgatgcga
      721 ggcgattata tagaatgtgc atatctgcgg cgctctccga tatgggcagc ggaatgagca
      781 ggatcttccc gtcttccata tcatcaagat ggctcagtct ctcgaggacg actccttccc
      841 ccacaacctc cttagatacc atccaacctt gcataggcgc tggggcgtat tgttctacat
      901 aattaaagtt cgataccaga ttgccaactg cgtctacgat ctgaagctcc ggagcacgcc
      961 gaaacatccg caccggctgg acatatggct gcagataacc atcgaggctt tcagggcaaa
     1021 caagcccgag tcggcggaaa tagtcggcta cgtattctac tgcatcccaa ttcccttggg
     1081 tgccgggaag acgacccata tacttttcag aagcgagttc tcggatgatt ccgtctatgt
     1141 tgtttaaatc tacgctgaag tcaggcgtac catggcattc agaaccaggg tacatattgg
     1201 ctgagcaaat tcggggcgca cagagcgcca cgactacaac gataagcagc acccctttgg
     1261 ctttggttcg cacggatagt caccccctcc gataagccag actttttgct gcgacgaaac
     1321 aagaccttac gacctgtatt aattgccact tcttctttca ttgtccagat ccttctcagt
     1381 aagtagagtc agttgcagtc tgccgatgga tcgctaaagc tctcaaacgt tcgcacgggt
     1441 atctataata taccagcttg gcaagatcct tgcgctgcct ccatcccatg agaccgctga
     1501 ctcgttgtaa gggacgccct gaagcatgtc acgaagggct ctgattggct catagtggat
     1561 caggaaaagc acttgtcttg gagatggcat atcgcagacc ccgcgctact tgggtgtggt
     1621 accgtggagc ggccctacag ctattacact cttcgtacaa aggaccccgg tcgccgagtt
     1681 gccagcatcc tatttcatcc gacatgacag cacacgcccg attaggccgc aagggatct
//
LOCUS       DAOSAI010000426         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_46459_length_1724_cov_3.197723, whole genome shotgun sequence.
ACCESSION   DAOSAI010000426 DAOSAI010000000
VERSION     DAOSAI010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46459_length_1724_cov_3.197723"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PLM83_10920"
     CDS             <1..832
                     /locus_tag="PLM83_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOK71956.1"
                     /translation="TARRFRRRMEGLDRKSVGIALGSGAARGMAHIGVLEVLFHEGIH
                     IDVVVGCSMGAVVGGCYASGMEAEAIEEVAVSINKLDVARHVDLVSPTSGGLMAGSKL
                     SALLKELTGEKTFDELRFPFACVAADILTGDEVPIDDGYVYEAMRASSSIPGVFQPVV
                     HGKRVLVDGGILNPVPANYLRALGVDLAIAVDVMPVLRPSEEQLGRVPSIPSTLINSF
                     DIMGRLVAAPLTRLADVVIKPDVGEVFGAELWRAPELIEKGREAAKAAIPAICKALKV
                     "
     gene            899..>1724
                     /locus_tag="PLM83_10925"
     CDS             899..>1724
                     /locus_tag="PLM83_10925"
                     /inference="COORDINATES: protein motif:HMM:NF022604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HOK71957.1"
                     /translation="MEYDSINVDMSQAWAGGSFSERETMGVMEEMRFWLRIMSDHAKL
                     IRNGFDLAEEELFRFADALAENLDGMLARVQSVNPPFSPGEVQSFIAESNAVVVPLRE
                     FKADLEVAIRECRVMTDRPAELIDHVRREAEFFLGIINGLVGGPIPTRCNLKIPAPPG
                     KESEPAALAPRSLISCMGQFAQSILLDGILFFTHINGEHAQVLSIYFRPEIQEELAET
                     TKGYGNRLLCLLDEMTGKIVQGSSFQEMKDVVTRIREATIPWLEHLRTLKTKIGSCS"
BASE COUNT          388 a          413 c          516 g          407 t
ORIGIN      
        1 gactgccagg cggtttcgca ggagaatgga agggctcgac cggaagtctg tcgggattgc
       61 gctcggaagt ggagcagcgc gcgggatggc ccatattggt gtgctcgagg tcctttttca
      121 cgaaggtatt cacatagacg tcgtcgtggg atgtagcatg ggcgccgttg tgggtgggtg
      181 ctatgcctct ggcatggaag ctgaagccat cgaagaggtg gctgtttcca ttaacaagct
      241 ggacgtggct cgccatgttg accttgtttc tccaactagt ggaggactga tggctggctc
      301 gaagttgagc gcgctgttga aagagctcac tggggagaaa accttcgacg agctgagatt
      361 cccttttgca tgtgttgctg cagacatact aaccggtgat gaggtgccga tcgatgatgg
      421 atacgtctat gaagcaatga gggcttcgtc ctctataccg ggcgtatttc aacctgtcgt
      481 ccatgggaaa cgtgtactgg ttgacggcgg aatactcaat cctgtgcccg ccaactatct
      541 tcgcgccctt ggggtcgacc tcgctattgc agtggacgta atgcccgtgt tgaggccatc
      601 cgaggaacag cttggccgtg ttccgtcaat accttcaacc ctaataaact cctttgacat
      661 catgggaagg ctcgttgcag cgcccctcac acgccttgcg gatgtagtta tcaagcccga
      721 cgtgggtgag gtcttcggcg ctgaattatg gagggccccg gagctcattg agaaagggcg
      781 ggaggctgca aaggcagcca tcccggcaat atgcaaggct ttgaaagtct gaactgtcat
      841 gaacctgcca cttgccgact atcgtattgt gataaggcaa gccggaggtg aatgtagcgt
      901 ggaatatgat tccatcaacg tcgatatgtc tcaggcgtgg gctggaggat cgttttcgga
      961 gcgggaaacc atgggtgtca tggaggagat gcggttctgg ctccgcataa tgtcggatca
     1021 cgccaaactg attagaaacg gattcgatct agcggaagaa gagctattcc gctttgcgga
     1081 cgctttggca gaaaacctag atggaatgtt agctcgggtt cagtctgtaa atcctccatt
     1141 ttcgcccggc gaagttcaat ccttcatagc tgagtcaaat gccgttgttg tgcccctcag
     1201 ggagttcaag gccgacctgg aagtggcaat ccgggagtgc cgagtgatga ccgatcgtcc
     1261 agcagagctt attgaccacg taaggagaga agctgaattc ttcctcggga ttataaacgg
     1321 tttggttggc ggcccgattc ccactaggtg caatctaaag attcctgccc cgccggggaa
     1381 ggagagcgag cctgcagcgc ttgcgccgcg cagcctcata tcatgcatgg gacaatttgc
     1441 acagtcgatc ctcctcgacg gtattctgtt cttcacgcac atcaacgggg aacatgccca
     1501 ggttctgtcc atttacttca ggcctgaaat ccaagaggaa ctggcggaaa cgaccaaggg
     1561 ttacgggaac agacttctat gtctgctaga tgagatgaca ggcaagatcg tgcagggttc
     1621 gagttttcag gagatgaaag atgtggtgac tcggatcagg gaagctacga taccctggct
     1681 cgagcatctt cggactctca agactaagat cggttcctgc agcg
//
LOCUS       DAOSAI010000427         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_46670_length_1718_cov_3.190619, whole genome shotgun sequence.
ACCESSION   DAOSAI010000427 DAOSAI010000000
VERSION     DAOSAI010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46670_length_1718_cov_3.190619"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLM83_10930"
     CDS             complement(<1..279)
                     /locus_tag="PLM83_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOK71958.1"
                     /translation="MDKEGMVNAKEYGRRSKPRRAKSRPLAIRVNFEKAAFILAAMVL
                     ATVGLTLAFSQRPYVAPPLENLETVTVTVHPGDTLWGIASRFSDGNVDI"
     gene            428..1045
                     /gene="lexA"
                     /locus_tag="PLM83_10935"
     CDS             428..1045
                     /gene="lexA"
                     /locus_tag="PLM83_10935"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004890619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOK71959.1"
                     /translation="MAMLSAREEQILQLISDQIEERGYPPSVREIGQAVGLKSTSTVH
                     GYLSSLERKGYIRRDAAKPRAIEVIMGVDRMPLPARDVLNVPLVGRVTAGQPILAVEN
                     IADYFPLPRSLAGECDSFLLQVSGESMVGAGILDGDYVLVRKQPTAENGDIVVALIED
                     EATVKRFYRDGNHVRLEPENPAFSAIITDKAMLLGKVIGLVRKIH"
     gene            complement(1141..1500)
                     /locus_tag="PLM83_10940"
     CDS             complement(1141..1500)
                     /locus_tag="PLM83_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71960.1"
                     /translation="MIHLAREDVLRGLERFRSIAKQDFLASGLTENPEFWAAQAAARR
                     AQYDQLIEIIETSGVPRAIEKAIECYNELPNLYDEPEFGQPETRGRKQALEVFFKAVG
                     VDRKTIRDARGQKTARM"
     gene            complement(1532..>1718)
                     /locus_tag="PLM83_10945"
     CDS             complement(1532..>1718)
                     /locus_tag="PLM83_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71961.1"
                     /translation="SSASIYKVVATTKCVSTRFENVCLGSRRMLVAYSFGTIGREQGK
                     EESADQWRTIKPTGVNL"
BASE COUNT          379 a          476 c          435 g          428 t
ORIGIN      
        1 aatgtcaaca ttgccgtctg agaaccgcga cgcaatgccc catagcgtgt cccctgggtg
       61 cacagtcacg gtgaccgttt ctagattttc tagcggcgga gccacgtatg gcctctgaga
      121 gaacgccaat gtgaggccta ctgtggcaag caccatcgca gctaggataa atgcagcttt
      181 ctcgaaattg acgcggatgg cgagcggcct cgatttggcc ctgcggggtt tggatcgtct
      241 gccatattcc tttgcattca ccattccctc tttgtccaca gatgcttccc ccttggcaaa
      301 cagatgttct ggacccaatc caaggttaac agaacatatg ttctgtgtca agggctgaac
      361 cgaacgaatg tttgcaacag ctcggctgct gtgatatact ttcctcagct gaagggggtt
      421 gatgggcttg gctatgctta gcgccagaga agaacagatc ctccaactca tatccgatca
      481 aatcgaagag cgaggctatc cgccatcggt cagagagatt ggtcaagctg tagggttgaa
      541 atcgacctct actgtccacg gatatctctc atccctcgaa cgtaagggat acatacgccg
      601 cgatgcggcc aagcctcgag caatagaagt gatcatgggc gtcgaccgca tgcctctgcc
      661 cgctcgcgat gtcttgaacg ttcccttggt ggggcgggtc actgccgggc agcctattct
      721 cgctgttgag aacattgcgg actacttccc tctcccccgc tctttagctg gggagtgcga
      781 ttccttcctg cttcaggtgt ccggcgaaag catggtgggt gcaggcatac tcgatggcga
      841 ctatgttctt gtcagaaaac agcccaccgc ggaaaatggc gatatcgtag tggctttaat
      901 tgaggatgag gcaacagtca aacgcttcta tcgcgacggt aatcatgtga ggctcgagcc
      961 ggagaaccca gccttctctg ccataatcac cgacaaagcc atgcttttgg gaaaggttat
     1021 cgggctggta aggaagattc attgattttt gtggaccaca gcgaagccgg gctggattca
     1081 tcccgcccgg cttctatatg tttacagagg aaatgccctg tataaaaaag caaaacgcgc
     1141 ctacatgcgc gccgtctttt gcccccgagc atcccttatt gttttgcggt ctacgccaac
     1201 cgctttgaag aagacctcaa gcgcctgttt tctaccacgc gtctccggct gaccgaattc
     1261 gggctcatca taaaggttcg gcagttcgtt gtagcattct atcgccttct ctatggcccg
     1321 cggaactcca gatgtttcaa taatctctat cagctgatcg tactgtgccc gtcgggctgc
     1381 tgcttgagcc gcccaaaact ccggattttc cgtgaggccg cttgcgagga aatcttgctt
     1441 cgcgatgctc ctgaaacgct caagcccccg cagaacatcc tcacgcgcga ggtgaatcat
     1501 ctatcattcc cccttgtcgg attttgtggg actaaagatt cactccagtt ggtttaatcg
     1561 ttcgccattg gtcggcagat tcctccttgc cctgctcacg accgattgta ccaaaagaat
     1621 aggcaaccag catacgccgg ctgcccaggc agacgttctc gaatcttgtc gacacgcatt
     1681 ttgttgtagc taccacctta taaattgacg ccgatgat
//
LOCUS       DAOSAI010000428         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_47025_length_1709_cov_3.405683, whole genome shotgun sequence.
ACCESSION   DAOSAI010000428 DAOSAI010000000
VERSION     DAOSAI010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47025_length_1709_cov_3.405683"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..1272)
                     /locus_tag="PLM83_10950"
     CDS             complement(373..1272)
                     /locus_tag="PLM83_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOK71962.1"
                     /translation="MEREVEPKFRIIYYPFMGTSVNTERLILLARLCYKPIGVDGLYD
                     MVWGTDSDPVDPGLQHMDDEAVGKFVRGLIRSGHLGALEHWNTSFAVEGYSRISSQQN
                     DRHRLMKTFKDPGVLQAEAAPEGGRAKEACEAWVNGPEQGSADVSQMQQSQRYVREDD
                     FKYVVPPSFHKDPEFLARYRKLQEDIAELQRYGLNEAKLPAEDVRFGLSNATETRFVI
                     TTNARQLRHMFNLRCCQRAQWEIRDLFDGLLREYKKIAPNIFWRAGASCEDFGYCPEG
                     SASCGRAPTIDQLLDSAGRKRED"
     gene            complement(1312..>1709)
                     /locus_tag="PLM83_10955"
     CDS             complement(1312..>1709)
                     /locus_tag="PLM83_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOK71963.1"
                     /translation="RYCRIEIREVAESPSDSANGEARKSQLRADEAVRLISRIRPGSY
                     VIACDLAGKEMTSEQFAQFISDLGIAGVSDLTFVIGGPLGYSPEVLAGANQRIAFSKM
                     TFPHQLMRVILLEQIYRAFKIARNEPYHH"
BASE COUNT          364 a          530 c          403 g          412 t
ORIGIN      
        1 agatagtccc ggcctgccgt caatccgatc tgaacaccat tgccatgcca gacttggtcc
       61 tcagagctct tgcaacacgc cgcatgtgcc tgctcgctac tgtgctcgcc ccatagcgta
      121 ttcccctgta cccatctgaa ctgagttttg agagaaccct ttgcgttcgg gcatttctcg
      181 ctcatccccg tatgatgaag cttggaatat gaggagcgac gccacgataa cgcctgtctt
      241 tttatgtatc cagcatgaaa cggcccaatg tgggtcgttt ttgcaaagtg acttggtgtg
      301 tcagaacccc ggccctcaga cgctcctagg tggcattcaa gtcatcgaaa tggccgtaaa
      361 gccgcatatg ggtcagtctt ctctcttgcg ccctgcgctg tccaacagtt ggtcgatggt
      421 aggcgcccgc ccacagctgg ccgatccttc cggacaatag ccaaaatcct cacaggaagc
      481 gcctgcccgc cagaaaatgt tcggagcgat cttcttatac tcccgcagca gcccgtcgaa
      541 gaggtcgcgt atctcccact gcgccctctg acaacacctg agattgaaca tatgccgcag
      601 ctgtctggca ttggtagtaa tcacaaacct cgtttccgtg gcgttggaga gcccgaacct
      661 cacatcctca gccggaagtt tcgcctcgtt caagccgtat ctttggagct ccgcaatatc
      721 ctcctgcaac ttcctgtatc tcgcgaggaa ctcgggatcc ttatggaaac tcggaggcac
      781 aacgtacttg aaatcatcct cccggacata cctctgggat tgctgcatct gagatacgtc
      841 ggccgagccc tgctcaggtc cgttcaccca ggcttcacat gcctctttcg cccggccccc
      901 ctcaggggcg gcctcggcct gcagcacacc tgggtccttg aacgtcttca tcagcctgtg
      961 cctgtcattt tgctgactcg atattctgct gtatccttcc acggcaaagc tggtgttcca
     1021 atgttccagt gccccgagat ggcctgaacg tatcaggcct cgcacgaact tcccgacggc
     1081 ctcgtcgtcc atatgctgaa gcccagggtc aacaggatca ctgtccgtgc cccacaccat
     1141 gtcatacagc ccatccactc ctatcggctt gtagcacagc ctggccagga gaatcaaacg
     1201 ttctgtgttg acagatgtcc ccatgaacgg atagtagatt attctgaact tgggctcaac
     1261 ctcccgctcc atactaatca ctccctaagc gcacagctta tccacagctg atcaatgatg
     1321 atatggttca ttccttgcta ttttgaaagc cctgtatatt tgctccagaa gaatcacccg
     1381 catcaactga tgcgggaatg tcatcttcga aaaggctatc ctctgattcg ctccggcaag
     1441 gacctcgggg gaatatccaa gaggacctcc gatcacgaaa gtgaggtcgc ttacgcctgc
     1501 gatgccgaga tcggagatga actgagcgaa ctgctcagaa gtcatctcct ttccggcgag
     1561 gtcacaagct attacatacg agcctggccg gattcgcgat atcaacctga cagcttcgtc
     1621 tgcccgaagt tgactttttc tggcttcgcc gttcgccgaa tcggacggcg actccgccac
     1681 ctcgcgtatc tcaattctgc aatatcggg
//
LOCUS       DAOSAI010000429         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_47913_length_1688_cov_3.434783, whole genome shotgun sequence.
ACCESSION   DAOSAI010000429 DAOSAI010000000
VERSION     DAOSAI010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47913_length_1688_cov_3.434783"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLM83_10960"
     CDS             <1..325
                     /locus_tag="PLM83_10960"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOK71964.1"
                     /translation="LEAVDEAKSYEPLPRYPAIERDVAMVLPRAVSIADVAELIEKTG
                     GELVRSVTLFDVYEGLPVPEGKRSIAFTVTYRAQDRTLTDEEVNRLHEEVRKALTDEM
                     GAVLR"
     gene            446..724
                     /locus_tag="PLM83_10965"
     CDS             446..724
                     /locus_tag="PLM83_10965"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOK71965.1"
                     /translation="MLTEQGPDGLDQEGVRVSAVILGEEYVIRGDTEAEQIESLAKEV
                     DLRMKAIAEANPRLSSQQVAVLSALNLMGELVVSKRRYSDLLDLLEQV"
     gene            743..1312
                     /locus_tag="PLM83_10970"
     CDS             743..1312
                     /locus_tag="PLM83_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOK71966.1"
                     /translation="MTVSSLDWLSIVLIVILVADFVVGYRRGFVRQLFDFAGIIIAIL
                     VAANRWQGASGLLQKWFPKLRPPGAHMVGFLALLIITLVVVDLIGQALSSAVSASGVS
                     ALNCFGGAALRILRSCLIISIVLVALVSLRIPSLTRTVESSPVAMKIIKVAPSIWDGF
                     EKFMPEQFRPSGPGGAKGEPAKPGGGGFI"
     gene            1275..>1688
                     /locus_tag="PLM83_10975"
     CDS             1275..>1688
                     /locus_tag="PLM83_10975"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOK71967.1"
                     /translation="MNQLNLAVAASFSEIADLLEIMGDNPFKVRAYRRAAEAIAGLDE
                     PASEILARDPGSIPGVGDALRAKVEEMESTGRIEYLEKLRLEVPAAILALIEVPGIGP
                     KTAWKLYESLGVEDEASLLAAARAGKIRDLPGFGEK"
BASE COUNT          351 a          388 c          544 g          405 t
ORIGIN      
        1 gttggaagcc gtagatgaag cgaagtcata cgagccattg ccgaggtatc cggcaataga
       61 gcgtgacgtg gcaatggtgt tgcctcgcgc agtctccatt gcagatgtgg ctgagcttat
      121 cgaaaagacc gggggggagc ttgttcgctc tgtcacgctg ttcgatgttt acgagggctt
      181 gcctgtgcct gagggaaagc ggagcatcgc ctttaccgta acctaccgcg cccaggaccg
      241 caccctcact gatgaagaag tcaaccgcct gcacgaggag gtcaggaagg ctttgacgga
      301 cgagatgggc gcagtcctta gataaaacat gtgcgtgcga gaaaatgcag gaatctgcaa
      361 tattgttggc aaacaatcta cgtgatagga cctgattgcc gaggggtgtc aggagccgca
      421 tcatggatgc aggagggtga tgctgatgtt gacggagcaa ggtcccgatg gattagatca
      481 ggaaggcgtg cgggtgtccg cagtcatctt gggtgaggaa tacgtgatac gcggggatac
      541 cgaggcagaa cagattgaat ccttggctaa ggaagttgac ttgcgaatga aagcgatagc
      601 ggaggccaat ccgaggttgt cgtctcagca agttgctgtg ctctctgccc tcaatctcat
      661 gggagaactc gttgtgtcaa agcgtcgcta ctctgatctc cttgacctgt tggagcaggt
      721 ttagatacgg aggtgaggct ttttgactgt ctcctctctt gattggctta gcatagtcct
      781 catagtgata ctcgttgcag acttcgttgt cggctatcgt cgcggatttg tgcggcaact
      841 attcgatttt gcgggcataa taatcgcaat cctcgttgcg gccaaccgtt ggcagggcgc
      901 ttccggtctt ctgcagaaat ggtttccaaa actgcggcct cccggcgctc acatggtagg
      961 ctttttggca ttgctcataa tcactttagt agtggtcgat ttgatcgggc aggcattgtc
     1021 atcggccgtg agcgcctcgg gagtgtcggc gcttaactgc tttggcggcg ctgcgctgag
     1081 aatcctcagg tcctgtctta tcatcagcat cgtgctggtg gctctggtct cgttgaggat
     1141 acccagcctt accaggactg tggagtcttc tccggttgcc atgaagataa tcaaggttgc
     1201 gccttccata tgggacgggt ttgagaagtt catgccggag caatttcgtc cttcgggacc
     1261 tgggggggcg aaaggtgaac cagctaaacc tggcggtggc ggcttcattt agcgagatcg
     1321 cagatcttct cgaaatcatg ggggataatc cgttcaaggt aagggcgtac aggcgagccg
     1381 ccgaggctat cgctggattg gacgagccag cctccgagat ccttgcgcgc gatcctggga
     1441 gcatacccgg ggttggggat gctcttcggg caaaggtgga agagatggag tctaccggca
     1501 gaattgagta tctcgagaag ctcaggctcg aagtgcccgc cgctatattg gcgttgatag
     1561 aggtgcctgg tattggtccg aagactgcgt ggaagctcta tgagtccttg ggagtggagg
     1621 acgaggccag tctgctggcg gctgcgcggg cggggaagat tcgggatttg ccagggtttg
     1681 gcgagaag
//
LOCUS       DAOSAI010000430         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_48040_length_1685_cov_4.118405, whole genome shotgun sequence.
ACCESSION   DAOSAI010000430 DAOSAI010000000
VERSION     DAOSAI010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48040_length_1685_cov_4.118405"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLM83_10980"
     CDS             <1..480
                     /locus_tag="PLM83_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71968.1"
                     /translation="MVPRVSSRWFRLLLMGVGVLALPASRAAVPAPTSAAAVAAAPWQ
                     PPPPPPDDFDWIQLKSGEWLKGRIKALQEDKLEFDSEELERQTMLERGLVSGVVAEAA
                     DARRRYGDCFGDRLGGQEPEGKRHEVGDWQICPERYPSHRRSVSFSNAHNRRRRSHG"
     gene            446..727
                     /locus_tag="PLM83_10985"
     CDS             446..727
                     /locus_tag="PLM83_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOK71969.1"
                     /translation="MPITAGEEVTVKYNGLLAQSGADSVYLHAGFGAAGSWHDVVDIA
                     MLQEKPGVWKATLPLVPEETSRLNFCFKDRAENWDNNYGLNWSFDIHNG"
BASE COUNT          423 a          414 c          501 g          347 t
ORIGIN      
        1 atggttccgc gcgtatcatc ccggtggttc cggctcctgc tgatgggcgt cggcgtgctg
       61 gcccttcccg ccagccgggc cgccgtgccg gccccaactt ccgccgccgc cgtggccgca
      121 gcgccttggc agcctccccc tccgccgccg gatgatttcg actggatcca gctcaaatcg
      181 ggcgaatggc tgaaggggcg gatcaaagcc ctccaggagg acaagctcga gttcgacagc
      241 gaggaactcg agcggcagac tatgctcgaa agggggttag tgtcgggtgt tgtggcagag
      301 gcggcagatg ccaggcgccg atatggagat tgcttcggag acagacttgg gggccaagag
      361 cccgaaggaa agaggcacga ggtcggggac tggcagatct gcccagaaag atacccatct
      421 cacaggcgga gtgtcagttt ctccaatgcc cataaccgca ggagaagaag tcacggttaa
      481 atacaatggc cttttggccc agtcgggtgc ggatagcgtc tatttgcatg caggattcgg
      541 ggcagccgga agttggcacg atgtggttga cattgcgatg ttacaggaga agccaggcgt
      601 atggaaggca actttgccat tggttcccga agagacgtct cgactcaatt tctgctttaa
      661 ggaccgagcc gaaaactggg acaataatta cggcctgaac tggagctttg atatacacaa
      721 cggatagatc cgaatgccat ggatgcttcc cgttcccgtg ccgcctgggc gggttcctca
      781 gaagttcgat tattggccgg ggcgacaatg gcaagcagag gcgaggccca cgctcgcttc
      841 tgttcttgta tttacccaga aacagttgcc agaagaggag ttcaacgttt aaggcggcgc
      901 atgaggaagc actagaaaat gctcatgtct ggacctcgcc ttttaggagg aatctgccgc
      961 atgaatatca agaagcatcc atggaattcc gtaagcctcg tattctgacg cgtgttggag
     1021 catcgagtca tcgtgggtgg ggatgattgc cgatacgccg catacttcga aaataacggc
     1081 tgcaattgcg acttatcata agagcagtat gaggggacaa ttccccgctc aggagcaaaa
     1141 agaatcttag caactgaggc cgaagctatt cgacattcca cacgctaagt gaaagccaag
     1201 atgcggggaa gatgaatagg ctgaaacagt ctgtggacta tgcaatccgg gtgtataggc
     1261 ttttcgatat gatcaacgga tatttagatg agggctgaac atcagaacta cagatactgc
     1321 gacattcgac gatgctctgt atgtccagac tccatttaca aatgtcgtga acgcttggaa
     1381 caaataggtt gttatgaagg tgaaacgcga gagctaccac ctagtgcgcg atggggtgac
     1441 gccgaatggg gaaatcatat cgaacaatca tgaagttacc tagtctgcgc acaagcacac
     1501 ctgaaaaacg cgactgcgcg gggtgtaacg ctgaaccacc ggcaagttgg ctagctggtg
     1561 cgggagactc catccgccaa agcagaacaa atgggggata ggggaccgta gcagcacaat
     1621 acgtggccag gccgaaacgg atatgcgaaa ccctgtattt tcgttttgcg agttgctcga
     1681 acact
//
LOCUS       DAOSAI010000431         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_48749_length_1668_cov_2.643521, whole genome shotgun sequence.
ACCESSION   DAOSAI010000431 DAOSAI010000000
VERSION     DAOSAI010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48749_length_1668_cov_2.643521"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLM83_10990"
     CDS             complement(<1..237)
                     /locus_tag="PLM83_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71970.1"
                     /translation="MDGIPVASTRSLTAVPDTVPQLEALVYGAVELKEAPAAILDMRS
                     YMGQNDWYAMEWVRRFAGVDVCPASFHAQLVTDTA"
     gene            complement(370..603)
                     /locus_tag="PLM83_10995"
     CDS             complement(370..603)
                     /locus_tag="PLM83_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71971.1"
                     /translation="MTLLQRGLGFIQDGHFSIHPKTMCRRRRFFARFDTEFDECGGRF
                     YARRRPDLWVAAVEGEDPSRWMKPSIGLDGEVF"
     gene            complement(600..971)
                     /locus_tag="PLM83_11000"
     CDS             complement(600..971)
                     /locus_tag="PLM83_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71972.1"
                     /translation="MTIRRAYAAFAAAVIIGCVLMPMMTVKALAVEPGIPGVSGSEAL
                     EEFENRSYKYDNFWGEPYAGSILSKQHACEPLPGFISKDDSLYDVDVLFRVLKYGYAG
                     YQSFGGDEVLLTGRAWDPTNS"
     gene            1151..1393
                     /locus_tag="PLM83_11005"
     CDS             1151..1393
                     /locus_tag="PLM83_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOK71973.1"
                     /translation="MIINTKQMIPLADANRNFSKVARAADQQGAVVILKNNVPKYVVL
                     SFDHFEGLSKSEDERLESVAARILKENLPAFSELAK"
BASE COUNT          419 a          476 c          429 g          344 t
ORIGIN      
        1 ggctgtatcc gtcaccagct gagcgtggaa gctggcaggg cacacatcaa ccccagcgaa
       61 ccttctcacc cactccatgg cgtaccagtc attctggccc atgtacgatc tcatgtccaa
      121 gatagccgcg ggagcttcct tcagctcgac cgcgccatag accaacgcct caagctgtgg
      181 cacagtgtca ggcaccgccg tcaggcttcg agttgacgca acgggaatgc catccatata
      241 cctaagctcg tacgcaggct tcggggcaaa gccggaatca accaggttga gccagataaa
      301 ctcatacgac ccatcgctcc ggccaagctt gacacatatg agattggcat cgcttatggc
      361 tccgaggcac tagaagacct ctccatcgag gcctatagac ggcttcatcc aacgcgatgg
      421 atcctcccct tcgaccgcag ctacccatag gtcgggccta cggcgtgcat agaacctgcc
      481 gccgcactcg tcaaactccg tgtcgaatcg tgcaaagaac ctgcgtctgc ggcacatggt
      541 cttgggatgg atggagaaat ggccatcctg aatgaaacca agtccccgtt ggagcagtgt
      601 cacgaattcg ttggatccca ggctctcccc gtaaggagta cctcatcgcc gccgaaagac
      661 tgatatccag cgtatccgta cttcaacacc ctaaagagaa cgtcgacatc gtacagggaa
      721 tcatccttcg agatgaatcc gggcagcggt tcacaggcat gttgcttgga gagtattgat
      781 cctgcatacg gctcacccca gaagttgtca tatttgtatg accgattctc aaactcctcg
      841 agcgcctcgg atcccgaaac gccgggaatg ccgggctcca cagccaatgc cttcaccgtc
      901 atcatcggca tcagcacaca cccaatgatc acagctgcgg cgaaagcagc ataggccctg
      961 cgaatcgtca catagttcgc ctccttcctg tcgtcccaag aagaatgctc agagcagaaa
     1021 ctggggaatc gcggaagggc taatcgaggt gctctgccgg agcgcaacca agaatccgca
     1081 taatggcagt gggcaaatgt aggttcaatc ttgcctacat attgagtata ctggggatga
     1141 ggtgatcgac atgataataa acaccaaaca gatgatccct ttagctgatg ccaacaggaa
     1201 cttctccaaa gtggcacgtg cagcggacca gcagggagca gtggtcattc tgaagaacaa
     1261 cgtgcccaag tacgtcgtgc tcagttttga ccacttcgag gggttatcca aatctgaaga
     1321 cgagcggctg gaatcggtgg ctgcccgtat cttgaaggag aatctcccag cgttttcgga
     1381 gctggcaaag tgatccgact caactgcaag caagtgcttc accttcatca gcagatgatt
     1441 gcggtcacag gggggacctc aggcgtgcgc gaccagggcg cactcgactc ggctatcaat
     1501 cacgcttttg catcagttga aggcaaggat ctgtacccca cagtagaaga aaaggctgct
     1561 cgtcaatgct ttcgatagtc caaaaccatc cgttcgtaga tggcaacaag cgaaccggac
     1621 tcttcgtcat gctggttttt ctggatatga atggagtgaa gctgcact
//
LOCUS       DAOSAI010000432         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_49141_length_1658_cov_2.976294, whole genome shotgun sequence.
ACCESSION   DAOSAI010000432 DAOSAI010000000
VERSION     DAOSAI010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49141_length_1658_cov_2.976294"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLM83_11010"
     CDS             complement(<1..1171)
                     /locus_tag="PLM83_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOK71974.1"
                     /translation="MAARQGSQPTRTKKRSKPVRAKPKHAKKSAASQDYALGGELGSE
                     IGGIFLVAGAALGFISLIQGPSGYVGGALANLMISAFGRGAYAVPALMALTGVLMLWR
                     RTGHVVFVRFVGLVIVCIAVVIGFELNDFGETALRLPFSQYVADSRSDVGGGLVGAAG
                     AWVFLRLFGFVGSVIISIALGIAGLSIASDVPVATAVTFGAKSIWTGLSFLISMIRES
                     YDAIVLQLFEVAPPYPEDESAVDRADLAVQFREGDCLEANRRAKTAPEPEIGAHSQER
                     GLDRLKEFFQTSEPDAGIVERMEPQEDLPEPSRGELSVGANEVSVAPSKTRPRQSAQG
                     TKSQSIGSLHDEMEQLELINYGLASDLPPTSILKALPKRRARKEVEDPHAMAELLE"
     gene            complement(1204..>1658)
                     /locus_tag="PLM83_11015"
     CDS             complement(1204..>1658)
                     /locus_tag="PLM83_11015"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOK71975.1"
                     /translation="WCADCISSRQKQRKQESIDEGNIGYKQALGVGVAQACAIFPGVS
                     RSGLTISSALALKFERGFATSYSFIASLPVIVGSLILWPVANPGALAEVQLGTLLLAV
                     AASFVSGLAAMIFLRRVVVRGKLTYFSYYLWAIGIITILLHARGLLGL"
BASE COUNT          406 a          496 c          412 g          344 t
ORIGIN      
        1 actccagcag ttcggccatg gcatgcggat cctccacttc tttgcgagca cgacgctttg
       61 gcaacgcctt gagaatgctc gtcggaggca aatcggaagc aagtccatag tttatcagct
      121 ctagctgctc catttcatca tgcaaggacc cgatcgactg cgattttgtg ccctgagcag
      181 attgcctcgg ccgtgtcttc gaaggcgcga cacttacctc gttggcaccc acactcaact
      241 cacctctgga aggctcgggc agatcctctt gaggctccat tcgttccacg atgcctgcat
      301 caggctctga tgtctggaag aactccttga gtctatctag accccgttct tgagaatggg
      361 ctccaatctc cggctccggc gcggtctttg cccgcctgtt agcttcgagg caatcgcctt
      421 ctcggaactg aacagccagg tcggccctat caacggcaga ttcatcctcc ggatacgggg
      481 gagcaacctc gaacagctga agcactattg cgtcatagct ttctctgatc atgcttatga
      541 gaaatgacaa gcctgtccat atcgatttcg cgccaaaggt aacagcagtt gcaacaggaa
      601 catctgaagc aatggaaagc cctgcgattc caagggctat tgagataatc accgaaccca
      661 caaatccaaa aagcctgaga aatacccagg caccggcagc gcccacaagc cctcctccta
      721 cgtctgaacg tgaatcggcg acgtattggg aaaaaggaag cctgagagca gtctccccga
      781 aatcgttgag ctcgaagcct attacaacag caatgcacac gatcacgagc cctacaaacc
      841 ggacgaagac gacatgtccg gtccgacgcc agagcatgag aactccagta agcgccatca
      901 aggccgggac tgcgtatgcc ccgcgcccga acgctgatat catcagattg gctagtgccc
      961 cacctacgta acccgaaggg ccctgaataa gagaaataaa gccaagagcg gcccctgcta
     1021 ccaagaatat gccgccaatc tccgagccca gctcgccgcc gagggcatag tcctgcgaag
     1081 cggccgattt cttcgcatgc ttaggctttg cacgcactgg cttcgagcgt ttctttgttc
     1141 gggtaggttg gcttccctgt cgtgctgcca cacctcttca ccaccgtaag cacacaattc
     1201 tacttacagt cccaacaatc ctctagcatg caaaaggatg gtgatgatcc caatcgccca
     1261 gaggtagtat gagaagtatg tcagctttcc cctgacgacc acgcgtctca gaaagatcat
     1321 ggcagcgagt cctgatacga aagaggctgc tacagccaaa agaagagttc caagctggac
     1381 ttcggccaaa gctccggggt ttgccacagg ccagagtata aggctgccca caatcaccgg
     1441 caaagacgcg ataaacgagt atgacgttgc aaatcctctt tcgaatttga gggcgagcgc
     1501 ggacgagatg gtcaggcctg accgtgaaac cccggggaag atcgcgcacg cctgtgcgac
     1561 gcccacgcca agagcttgct tgtaaccgat attgccctcg tctatgcttt cctgtttccg
     1621 ctgcttttgc cgcgacgata tgcagtcggc gcaccata
//
LOCUS       DAOSAI010000433         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_50292_length_1632_cov_4.151554, whole genome shotgun sequence.
ACCESSION   DAOSAI010000433 DAOSAI010000000
VERSION     DAOSAI010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50292_length_1632_cov_4.151554"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLM83_11020"
     CDS             <1..410
                     /locus_tag="PLM83_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOK71976.1"
                     /translation="PGEEPRPLARIASGGEMSRVMLAIKSVLAVADAMPTMVFDEVDA
                     GIGGEAAIAVASKLANIGKVRQVLAVTHLPQIAAVADNHYAVSKLERSGRTEVTVEKL
                     DIEERVTEVARMLAGEAPSSVTVAHAREMLSRR"
     gene            519..830
                     /locus_tag="PLM83_11025"
     CDS             519..830
                     /locus_tag="PLM83_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOK71977.1"
                     /translation="MRLKDHPILDFSAGQEVAFTFDGKKLKGIDGEPIAAALHANGVR
                     VLRHSPVSGRARGFFCAIGNCSSCLMTVDGVPNVRVCTEPLREGMVVETQKGHGQLPI
                     D"
     gene            853..>1632
                     /locus_tag="PLM83_11030"
     CDS             853..>1632
                     /locus_tag="PLM83_11030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOK71978.1"
                     /translation="MNLLKEVDIAVIGAGPAGLAAALACAEAGASTLLIDANDVPGGQ
                     LVKHTHKFFGSERQHAGTRGIQIPELFAKKLVEHPNVETMWDTTASGYYDSGELVVED
                     GKRFIKILPKRLIVSTGASERFVLFENNDLPGVYGAGAVQTLMNVYGVVPGDRVLMVG
                     SGNIGLIVSYQLIQAGVDVAAVIEAAPCIGGYLVHAAKLRRAGVPILTSHTIVAAHGE
                     ECVTGATIAKVDEKWQATPGTECEIECDVICLAVGLMPLVDI"
BASE COUNT          368 a          365 c          545 g          354 t
ORIGIN      
        1 acccgggaga ggaaccgagg cctcttgcgc gtatagccag cggaggagag atgtcccggg
       61 ttatgttggc gatcaagtct gtgctagctg tagcggatgc catgccgacc atggtcttcg
      121 acgaagtgga tgcgggaatc gggggcgagg cggcgatagc tgtcgcgtca aagcttgcga
      181 atataggcaa agttcggcag gtgcttgcgg taacccattt gccgcaaatt gccgcggtgg
      241 ccgataatca ttacgctgtt tccaagctgg aaaggagtgg gaggacggag gtgacagtgg
      301 aaaagctcga tatcgaggag agggtgacgg aggtagcgag gatgctggct ggcgaggctc
      361 catcatctgt gaccgtggca catgcccggg agatgctttc tcggcgctag ggatgaagga
      421 gttggcatgc ctatgttgaa gaattgcgaa agaaagcatt ttgtgtcagg aggagggagg
      481 gcttgggccg cttcacaggt ggcctacggc cgagcaaggt gagattgaag gaccatccca
      541 tactggactt ttctgccgga caggaggttg catttacttt cgacgggaaa aagttaaaag
      601 ggattgatgg ggagccaata gctgccgctc tccatgccaa tggtgttcgt gtcctccgac
      661 acagtccagt tagtggacgt gcacgggggt ttttctgtgc tataggcaat tgctcgtcat
      721 gcttgatgac tgtagatggc gtgccgaacg tgcgtgtgtg cacagaaccg ctgagagagg
      781 gaatggtagt cgaaactcag aaaggccacg gccagctgcc catcgactga ggcgggccga
      841 gcagcaagga cggtgaacct gttgaaggaa gttgacattg ctgtgattgg cgcaggccca
      901 gcggggcttg cagctgcact ggcgtgcgcc gaagcgggtg cgtctactct gctcattgac
      961 gcaaatgatg tcccaggggg tcagcttgta aagcacactc ataagttctt tggatcagaa
     1021 cggcagcacg ctggaacaag ggggattcag attccggagc ttttcgcaaa gaagctcgtg
     1081 gaacatccca atgtagagac tatgtgggat acgaccgcat cgggctatta tgattccgga
     1141 gagctcgtgg tggaggatgg aaagagattc attaagattc tgccaaagcg cttgatagtg
     1201 tcgacaggcg cttccgagag attcgtcctt ttcgagaaca atgacttgcc cggagtgtac
     1261 ggcgccggcg cggttcagac tctgatgaac gtttatggag tcgttcccgg cgaccgggtg
     1321 ttgatggtgg gttcgggcaa tatcggactt atcgtcagct atcagcttat ccaggctggt
     1381 gtggacgtgg ccgccgtgat cgaggccgcg ccgtgcatag gaggctacct agttcacgcg
     1441 gcgaagttgc gcagagctgg agtgccaata ctcacttccc acaccattgt ggcagctcac
     1501 ggcgaagagt gtgtgacagg cgccactatt gcgaaggtag acgaaaaatg gcaagcaact
     1561 ccaggcactg agtgcgagat agaatgtgat gtgatatgcc tagccgtggg gctgatgccc
     1621 ttggttgata tc
//
LOCUS       DAOSAI010000434         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_50384_length_1630_cov_2.812698, whole genome shotgun sequence.
ACCESSION   DAOSAI010000434 DAOSAI010000000
VERSION     DAOSAI010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50384_length_1630_cov_2.812698"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="PLM83_11035"
     CDS             <1..1490
                     /locus_tag="PLM83_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOK71979.1"
                     /translation="HDQRDFEFAKKYGLPIKVVITPTGERLDESTMGGAYVDEGVQVN
                     SGQFDGMPSIEARSAIADYLEKEESGRRVVHYRLRDWLVSRQRFWGAPIPIVYCDSCG
                     IVPVPEEELPVMLPEHVKFMPTGKSPLMDCPQFVNTRCPVCGGPARRETDTMDTFICS
                     SWYFSRYASPKTDDGPWKRADVDYWMPVDQYIGGVEHAIMHLMYARFFTMALYDIGLV
                     GFEEPFTNLLTQGMVLMDGAKMSKSKGNTVDPDEIVNEYGADTARLFVLFAAPPERDL
                     EWSAEGVEGCWRFLQRVWRLVHQYVQAVGDERKNMHEGVGVAVVPNPDSLSPEAREVR
                     RAVHGALKKVTEDIEERISLNTAIAAIMECVNSLYALKDAVLSEQGNAAVMAEAIDIL
                     VVMLAPFAPHITEELWHEIGHSGSVHRVEWPKYDPRAIVVDEVEIAVQVNGKVRERLT
                     IAADATEDEAVAMALASEKVLAAVGSGSVVKTIFVPKRLINIVVK"
BASE COUNT          370 a          358 c          516 g          386 t
ORIGIN      
        1 cccatgacca gcgagacttc gaattcgcca agaagtatgg attgcccata aaggtggtaa
       61 tcacgcccac aggtgagcgc ctagatgagt ctactatggg aggcgcctac gtggatgaag
      121 gtgtgcaggt taactccggc caatttgacg gcatgccgtc gattgaggcg agatcggcca
      181 tcgctgatta cctggagaaa gaggaaagcg ggaggagggt ggtgcactat cgtctccgcg
      241 attggctcgt atccaggcag cgattttggg gcgctccgat accgattgtc tattgcgatt
      301 cttgtgggat agtcccggtt ccagaggagg aacttccggt gatgttaccg gagcatgtca
      361 agttcatgcc caccggcaag tcgcctctga tggattgccc tcaatttgta aacacccgat
      421 gccctgtctg cggaggacct gcgaggcgtg agacagatac gatggatact ttcatctgtt
      481 cgtcgtggta tttttcaaga tacgcgtcgc cgaaaacgga tgacgggcct tggaagcggg
      541 cggacgtaga ctactggatg cctgtggatc agtatattgg cggcgttgag cacgctatca
      601 tgcatctcat gtatgccagg ttcttcacga tggcccttta cgatatcggt ctggttggtt
      661 tcgaggaacc tttcaccaac ttgctcacgc aaggaatggt gttgatggat ggcgccaaga
      721 tgtccaagtc gaaggggaac accgtggatc ccgacgagat tgttaatgaa tacggggcgg
      781 atacagccag gcttttcgtg ctgttcgcgg cgcccccgga acgggatctg gaatggagtg
      841 ctgaaggcgt cgaaggatgc tggaggttcc tgcagagagt atggaggctt gtgcaccagt
      901 atgttcaggc ggtaggagat gagagaaaga acatgcatga gggagtggga gtggctgttg
      961 tgcctaatcc cgattccctc agccccgagg cgcgggaggt gcgcagggcc gtgcatggcg
     1021 cgctcaagaa ggttactgag gacatcgaag agcgtatcag cttaaacacg gcaattgccg
     1081 caatcatgga gtgtgtgaac tcgctgtatg cgttgaagga tgcggtgctg tctgagcagg
     1141 gaaacgctgc tgtcatggcg gaggctattg atatcctggt ggtcatgttg gcgccgtttg
     1201 cgcctcacat cactgaggaa ctttggcatg aaataggtca cagcggcagt gttcatcgcg
     1261 tcgaatggcc gaaatacgat ccaagggcga ttgttgtgga cgaggttgag atagccgtac
     1321 aggtgaatgg caaagttaga gagagactga ctatcgcagc tgatgccaca gaggatgaag
     1381 ctgtcgccat ggcgctggca agcgagaaag tgttggctgc ggtcggctcg ggttccgtgg
     1441 taaagacgat cttcgttccg aagaggctca taaacatagt tgtaaaatag tcttgttttg
     1501 tgatatagaa aattggtttt ttggctaacc tcattgcctg tgaggttagc cttttgttcg
     1561 tccggaatgt ttgtctagcc gacgagttac gttacgcaac cagcgggaag acaacccgca
     1621 gatgtgctat
//
LOCUS       DAOSAI010000435         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_51325_length_1610_cov_3.973633, whole genome shotgun sequence.
ACCESSION   DAOSAI010000435 DAOSAI010000000
VERSION     DAOSAI010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51325_length_1610_cov_3.973633"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..548
                     /locus_tag="PLM83_11040"
     CDS             129..548
                     /locus_tag="PLM83_11040"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOK71980.1"
                     /translation="MNDVKVYTDGASRGNPGAAAAAFVVYWPEGRVDHMGLYLGRTTN
                     NVAEYVALVAALRYILNRGVESFALFSDSELLIRQIKGRYAVRSARLKRLHEEAMSLL
                     SRFDSYRVNHVPREENFEADRWANETLDAARATMTAF"
     gene            571..1560
                     /gene="trpS"
                     /locus_tag="PLM83_11045"
     CDS             571..1560
                     /gene="trpS"
                     /locus_tag="PLM83_11045"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOK71981.1"
                     /translation="MMKRIFSGMRPTGKLHLGHLAGALTNWVRLQDEYECFFCIVDWH
                     GLMSEYADSKMIRENCIEVLLDWLAVGIDPRRSVVFMQSHVPEHAELHLALSMITPLG
                     WLERNPTYKEQILNMQNKDLGTYAFLGYPVLMAADILLYKAEVVPVGEDQSAHLEITR
                     EIARRFNSFFGEVFPEPQMLLTPTPRVPGTDGRKMSKSYGNAINISDPFDLIWEKLRT
                     MITDPARYRRTDPGDPDKCPVWDLHKVFTQDPNKRNELDRGCRTAGIGCIDCKKVLFE
                     NIKNALSPIQDKRRYYEAHREEFLDVLYDGAKKAKSVAKNVMEEVKVSVGLTL"
BASE COUNT          401 a          360 c          446 g          403 t
ORIGIN      
        1 agccaccggc tcataaccgg aaggttcctg gttcgagtcc aggtgggccc accactcttg
       61 gtttattcga tgaaggccac ctacctctgg tttggtggct tttctttatc gagcggaggt
      121 agtgcgcatt gaacgatgtc aaggtatata cggatggggc atctcggggc aatcctggag
      181 ctgccgccgc cgcttttgtg gtatattggc ccgaagggcg cgtcgatcac atgggcttat
      241 atttaggcag gactacaaac aacgtggccg aatatgtggc attggtggcg gccttgcgct
      301 acatcttaaa tagaggagtc gagagttttg ctcttttctc cgacagtgaa ttattgataa
      361 ggcagatcaa agggagatat gcagtgcggt cggcccgttt aaagcgcctt catgaggaag
      421 cgatgtcgct gttatctcgc tttgactcat atcgcgtgaa tcatgtgcct cgggaggaaa
      481 acttcgaggc cgatcgttgg gcgaacgaga cgctggatgc ggcaagggct accatgacgg
      541 cattttaacg atagacgagg tgacatcttt atgatgaagc gtattttcag cggaatgcga
      601 ccaactggca aactacatct gggacatctg gctggcgccc tcaccaattg ggtacgcctt
      661 caagatgaat acgaatgttt cttctgtatc gtcgactggc atggcttaat gtcagagtat
      721 gccgatagta agatgattag ggaaaattgc attgaagtct tgctcgattg gttagctgtc
      781 ggcatcgatc cgaggcgttc ggtagtcttc atgcaatctc atgtgccaga acacgccgaa
      841 ttgcatttgg ccctctccat gattacgcct ttgggctggt tggagaggaa ccccacatat
      901 aaagaacaga ttttaaacat gcaaaacaaa gatctcggca cttacgcctt cttgggttat
      961 cctgtcctca tggccgcaga tatcttgctt tataaggcag aagtggtccc tgtgggtgaa
     1021 gatcaatctg cccacctgga aataaccagg gagatcgcaa ggcggttcaa tagcttcttc
     1081 ggagaggtct tccctgaacc gcaaatgctc cttacgccca caccgagggt ccctggcaca
     1141 gatggacgga agatgagcaa gtcttacggg aatgccataa acatttcaga cccttttgat
     1201 ctcatatggg agaagctcag gacgatgatc accgacccgg ctcgttaccg tcgcactgac
     1261 ccgggagacc cggataaatg tccggtctgg gatctgcata aggtcttcac gcaagacccg
     1321 aacaagagga atgaattaga tcggggttgc cgtactgctg ggatcggttg catagattgt
     1381 aaaaaggttt tgtttgagaa tatcaagaac gccctttccc cgattcaaga taaaaggcgc
     1441 tattatgaag ctcacaggga agaattcctg gatgtattgt atgatggtgc taaaaaggca
     1501 aaaagcgtgg caaaaaacgt aatggaggag gttaaagtat cggttggcct cacgctgtga
     1561 cggatgcttt taaggtagaa atcgaggggt tttcgggccc gctcgagctg
//
LOCUS       DAOSAI010000436         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_51949_length_1598_cov_2.040830, whole genome shotgun sequence.
ACCESSION   DAOSAI010000436 DAOSAI010000000
VERSION     DAOSAI010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51949_length_1598_cov_2.040830"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PLM83_11050"
     CDS             complement(<1..839)
                     /locus_tag="PLM83_11050"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOK71982.1"
                     /translation="MDHLDDFARYLEAERNLSPRTVDAYIRDVGQFISFCEKSGTWQG
                     IAGTGVDAYQLSMLRKYLATLQSRGLAHSSISRKMSAIRSYLKFLAREGVIESNPTVA
                     MSQVRRPRRLPRTLSQQEVERLISVCGDGSPLDIRDRAILELLYSSGIRLAELVGLDV
                     KDYNASSGTVRVFGKGAKERIAPVGQAAVRALDDYIANARPHLASNSEETALFLNRGG
                     GRLSGRSVERMMKKRLMEAGLPQTATPHSMRHSFATHLTDSGADLRAVQELLGHADIS
                     TTQ"
     gene            complement(960..>1598)
                     /gene="trmFO"
                     /locus_tag="PLM83_11055"
     CDS             complement(960..>1598)
                     /gene="trmFO"
                     /locus_tag="PLM83_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOK71983.1"
                     /translation="MPIEEIARRGRDTLRYGPLKPVGLVDPRTGRRPYAVVQLRRENV
                     PGSLFNMVGFQTRLKQGEQSRVFRMIPGLEEAEFVRYGMMHRNTFVNGPEHLSATLQL
                     KESERIFMAGQITGVEGYMESVATGLVCGMNAGRLAMGKDPLTFPSKTAIGSLCSYVS
                     TPCKGRYQPMNVNFGLLPEPPRALKKNERRMWKVALARQSLHDFAGSLRGGC"
BASE COUNT          367 a          501 c          393 g          337 t
ORIGIN      
        1 atctgcgtag tcgaaatgtc ggcgtgcccg agaagctcct ggaccgcgcg gaggtctgcc
       61 cctgaatcag tcagatgagt tgcaaacgag tggcgcattg aatggggggt cgccgtctgc
      121 ggaagtcctg cctccatcaa gcgctttttc atcatccgct ccactgaacg ccctgagagc
      181 ctccccccgc ctctgttcag aaagagcgcc gtctcttcgc tgttggacgc aaggtgaggt
      241 cgggcattgg ctatgtagtc atcaagcgcc cgcaccgccg cttgtcccac gggagctata
      301 cgttccttag cgccctttcc gaaaacgcga acagtccctg acgaggcatt gtaatcttta
      361 acatccagtc ccaccagttc tgccaatctt atcccagacg aatacaacag ctcgaggatg
      421 gcccgatccc tgatatcaag cggggaaccg tcgccacaga cggatatcag gcgctccacc
      481 tcctgctgcg acagtgtccg aggcagcctc ctagggcgcc tgacctgcga catggcgaca
      541 gtagggttcg attcgataac gccctcccgc gcaaggaact tcaagtatga cctgatagcg
      601 ctcatcttcc tagatatgct gctatgggca agcccacggg attggagtgt agcaagatac
      661 ttcctgagca tgctcagctg ataagcatcg actcctgtgc ctgcaatccc ctgccacgtc
      721 ccgctctttt cgcagaagga tatgaattgc ccaacatccc taatgtatgc atccaccgtc
      781 ctaggagaca agttgcgttc tgcctccagg taacgggcaa aatcgtctaa atggtccaaa
      841 agaatccact ccacagccgt cccggcaaaa tcgcgctccg ccgacacgaa acccgacatg
      901 acggagaata ggttattctc gagcctgggc cgggattgca atacttgagg ccagcgcgcc
      961 taacagccgc ccctcaacga acctgcaaaa tcatgcaaag actgcctcgc caaagcaacc
     1021 ttccacatcc gtctctcatt cttcttgagc gctctcggag gctctgggag cagaccaaag
     1081 ttgacattca tcggctgata cctgcctttg cacggagtag aaacgtaaga acagagcgaa
     1141 ccgatggcag tcttgctcgg gaacgtcaat ggatcctttc ccatggccaa tcggcccgca
     1201 ttcatcccgc acacaagacc cgtcgcaaca gattccatgt aaccctccac ccctgtgatc
     1261 tggcccgcca tgaagatccg ctcactctct ttgagttgaa gggttgcgct caggtgttca
     1321 ggtccgttca caaaggtgtt cctgtgcatc atcccataac ggacaaactc cgcctcttcg
     1381 aggcctggta tcatccggaa aacacgactc tgctcccctt gtttaagcct tgtctgaaac
     1441 ccgaccatgt taaagaggga gcccggcaca ttctctctgc gcaactggac aacagcataa
     1501 ggtcgcctac cggtgcgagg atcgacaagg ccaaccggtt tcaaagggcc gtagcgcaga
     1561 gtatcacggc cccgtctggc aatctcctcg attggcat
//
LOCUS       DAOSAI010000437         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_53237_length_1570_cov_4.512211, whole genome shotgun sequence.
ACCESSION   DAOSAI010000437 DAOSAI010000000
VERSION     DAOSAI010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53237_length_1570_cov_4.512211"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="PLM83_11060"
     CDS             complement(<1..1301)
                     /locus_tag="PLM83_11060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOK71984.1"
                     /translation="MTKIKTDPVRLIPLGGIGEIGKNMLVVEYGDDMIVIDCGLMFPD
                     DDMFGVDLVIPDVSYLEDKKERIRAVILTHGHEDHIGALPYVLKRIGNVPVYGTRLTL
                     ALVQAKLREHGIQLGGNARVISAGDQESFGEISVEFIHVSHSIADCVALAINTPAGII
                     VHTSDFKFDQTPIDGRVMDLRRFAELGERGVLVMLSDSTNADRPGYTLSEKVVGELFR
                     DAFRDAEGRIFVTTFASHIERIQQALDAAAEVGRRVAIVGRSMENNVKLALDLGYLRV
                     KPGTIVDLDTLESLPPSRAVIMTTGSQGEPMSALTRMAMSDHRKVSIVPGDTVIISAS
                     TIPGNERMIGTTINRLFKQGAEVIHEAFSGFHVSGHASQEELKLMLNIVRPRYFVPVH
                     GEYRHLVKHARLAQEVGVPRSDILIPEIGDILEFSRERAEVA"
BASE COUNT          366 a          449 c          372 g          383 t
ORIGIN      
        1 gcaacttccg cgcgttcccg gctgaactcg agaatatcgc cgatctcagg tatgagtatg
       61 tcggaccttg gcactccgac ctcctgagcg agcctcgcat gcttaacaag atgcctgtat
      121 tctccgtgca cagggacgaa gtatcggggc ctaacaatgt tcagcatcag cttgagctcc
      181 tcttggcttg catggcccga aacgtgaaaa cccgaaaatg cctcgtgtat cacctcggcg
      241 ccctgtttga agaggcggtt gatagtggtt ccgatcatcc gctcgtttcc cgggatagta
      301 ctggcagata tgattactgt atcgcctggt acgattgaca cctttctgtg atcagacata
      361 gccattcgag ttagagcact catgggttct ccctggctgc cagtggtcat tatcaccgcg
      421 cggctcgggg gcagggattc caacgtgtca agatcgacta tggtgccggg cttgacgcga
      481 agataaccga gatccaaggc aagctttaca ttattctcca tcgagcgccc cacgatggct
      541 acccgccgcc ctacttcagc cgcggcatca agtgcttgct gtatgcgttc gatatgcgac
      601 gcaaaagttg taacaaatat ccttccctcg gcgtcacgga atgcgtcccg gaaaagctcg
      661 cccactactt tctctgacag agtgtatcca ggacgatcag cattggtaga gtcggaaagc
      721 atcaccaaga cgccgcgctc acccaattcc gcgaagcggc gcaagtccat tacccgccca
      781 tctatcggag tttggtcgaa tttgaagtca gaagtatgaa ctattatccc agccggggtg
      841 ttgatggcaa gagccacaca atcggcaatg gaatggctta catggatgaa ctccacactg
      901 atttctccaa agctctcctg gtcgcccgcg cttatgacgc gggcattgcc gcccagctga
      961 attccgtgct ccctcagttt tgcctggacc aaagccagag tgagccttgt gccgtacacc
     1021 ggtacgttgc cgatacgttt gagtacgtat ggaagggccc ctatgtggtc ttcatggccg
     1081 tgagtcagaa ttaccgccct tatgcgctct ttcttgtctt ccagatatga gacgtcaggg
     1141 atcaccagat cgactccgaa catgtcatcg tccggaaaca tcaatccgca atcgatgacg
     1201 atcatgtcgt cgccgtattc cacaaccaac atgttcttcc ctatttcgcc tattccaccc
     1261 agaggtatca agcgcacggg atctgttttg attttagtca ctacctatac tgcacctcgc
     1321 agattctcat tgtatcaagc ttctagaatt atatacctag cgccattgcg tatcaaggag
     1381 acagatcgac gcgcccattt tggcacatgc tttgcagcca cgccatctgg gtattcacca
     1441 tccatcaatt actggattgt ctgaccgact cgattctccc ccacccgcaa aagcataagt
     1501 catcaaagga ggccacttac aataagtggc ctcggaaaat gcaaatactc gagaggcact
     1561 gccattctat
//
LOCUS       DAOSAI010000438         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_53456_length_1566_cov_4.103905, whole genome shotgun sequence.
ACCESSION   DAOSAI010000438 DAOSAI010000000
VERSION     DAOSAI010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53456_length_1566_cov_4.103905"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..783
                     /locus_tag="PLM83_11065"
     CDS             133..783
                     /locus_tag="PLM83_11065"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOK71985.1"
                     /translation="MEREVREIIEEQLFSSEVILWAGRPRRGLALRASDWFLIPFSLV
                     WLCIATEITGNARKFGVPSVRYEFGLSGRRVFAQPLTVFEVVGWLFIAIGLYLLVGRF
                     FVDARVRRNTYYGVTDRRILILTGFFGNRFVSIPLDRVGEMNAIRRADGSGTIQFGRP
                     TYIPGWGDSTMNGHHYQNCRRVDPPSFEMIEDVLSVRNIIVSAQQGTYDGSGGAGG"
     gene            complement(861..>1566)
                     /locus_tag="PLM83_11070"
     CDS             complement(861..>1566)
                     /locus_tag="PLM83_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK71986.1"
                     /translation="RTMLGLESLTAGTVWRSPSCRFFYMDQHYETLDPNLTVLEAITE
                     AQSRGSAPGNDRGKQIQAARDLLASLLFRGADVHKRISVLSWGERARVSIARMLISNY
                     DLIALDEPTNNLDVQSRQAIESCLAEWKGTLVVVSHDRRFLEHTCNMIAAFNSGQVSV
                     FPGTYGEWEEHRKLEAAQAIGPAQKQGHTVLELEHRLALLSAKLSEKFQSDEEKVAIE
                     EEFLATARTLNRLRQK"
BASE COUNT          327 a          372 c          456 g          411 t
ORIGIN      
        1 gagacggcct gggagagtag gtagctgccg gattcttttt tatcttcaag agccttggct
       61 agatgccgag gccttttttg gcttcttccg ggaatatttc atatactcaa aggtgatgaa
      121 ggggggacga ttatggagcg tgaggtcagg gagatcatag aagagcagtt gttttcaagc
      181 gaggtcatat tgtgggcggg gcgccctaga agaggattgg cgcttcgggc ctcagattgg
      241 ttcctcattc cattcagtct agtttggttg tgcattgcga ctgaaatcac gggaaatgca
      301 agaaagtttg gagtcccctc ggtgagatat gaattcgggc tttcaggccg acgtgtgttt
      361 gcgcagccgc ttacggtgtt cgaggtagtg ggatggttgt tcatcgccat cgggctgtat
      421 ctcctcgttg ggcgcttctt tgtggatgcc cgggtcaggc gcaatacgta ctacggtgtg
      481 accgataggc ggattctcat cctgacaggt ttcttcggca acaggtttgt atctattcca
      541 ttggacaggg ttggagaaat gaatgctatt cgaagagctg acggaagcgg aaccattcag
      601 tttggcaggc ctacgtacat ccccggctgg ggcgattcta caatgaacgg ccaccattac
      661 cagaactgta ggcgagtgga cccgccctcg ttcgagatga ttgaggatgt tctttctgtc
      721 agaaacatca tcgtctcagc gcagcaaggg acatatgatg gatcgggagg ggcaggcggc
      781 tgacgccgag cataggaacc cggccctagc ttcaaggttg cacatgagcg ggccgggtct
      841 tcctgttcta tgcaaggagt ctatttctgt cgcagcctat tgagtgttcg ggctgttgcc
      901 agaaactctt cctctatggc taccttttcc tcatctgact ggaatttctc cgatagcttt
      961 gcagaaagca gcgcgagcct gtgctcgagc tcaagcacgg tgtggccctg cttttgggct
     1021 gggccaatgg cttgtgcagc ctcaagcttc ctgtgttcct cccattcgcc gtatgttccg
     1081 ggaaagacac ttacctggcc tgaattgaag gctgctatca tgttgcatgt gtgttcaaga
     1141 aagcgcctgt cgtgagagac gacgaccagc gttcctttcc actctgccag gcatgactcg
     1201 attgcctgtc gggattgcac gtcaagatta ttggtaggtt cgtcgagggc gattaggtca
     1261 taattggata taagcattct agcgattgat acacgagcgc gttcacccca gctcaatacc
     1321 gatatacgtt tgtgaacgtc tgcccctcga aagagcaggg atgccaatag atcgcgggcc
     1381 gcttgtatct gctttcctct gtcattgcca ggagcgctgc cccgagactg tgcctccgtt
     1441 atcgcttcca gcacagtaag gttcgggtca agagtctcgt agtgttgatc catgtagaaa
     1501 aaacggcatg aggggctcct ccatacagtg cctgcagtga gagattccaa tccgagcatg
     1561 gtgcga
//
LOCUS       DAOSAI010000439         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_53724_length_1561_cov_4.218459, whole genome shotgun sequence.
ACCESSION   DAOSAI010000439 DAOSAI010000000
VERSION     DAOSAI010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53724_length_1561_cov_4.218459"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..923)
                     /gene="dpsA"
                     /locus_tag="PLM83_11075"
     CDS             complement(30..923)
                     /gene="dpsA"
                     /locus_tag="PLM83_11075"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HOK71987.1"
                     /translation="MANLHGRHICLLGGDSRELKLVCALIDRGARVSCYGVPKDGLHP
                     EAIAAANAKEACRGANAIVLPLSGTDSNGNVRVTSSPICITRELLFVLSPNAILFSGI
                     VPRFLAAECQSLGIRTVETTANDELAILNSIPSAEGAILMAMQATPFTIHNSNCLVLG
                     LGRTGMTLARMLHGLGAHVWAAARKAEDRARAFEMGLSPLSFADLPSCLPQIDIAFNT
                     VPALVLDRSLVYSMRRTAAIIDLASSPGGVDFIAASERGIHAELAPGLPGKVAPVTAG
                     QIISKVLPEIIAAEFAKNKPD"
     gene            complement(1326..>1561)
                     /locus_tag="PLM83_11080"
     CDS             complement(1326..>1561)
                     /locus_tag="PLM83_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK71988.1"
                     /translation="WELKGQAMRELLSKLDDPTVETYDGYRRSFGDAWYMVLPDPVHP
                     SFMIYAEGSTGAEAERMLSAAREEIVAEMRKNV"
BASE COUNT          335 a          423 c          428 g          375 t
ORIGIN      
        1 tgttgtcttc ggaaatagtc acaacacccc taatcgggtt tgtttttggc gaattctgct
       61 gcgatgatct ccgggagcac tttcgagatt atctgccctg ctgtgacagg cgccaccttt
      121 cccggaagcc cgggggcgag ctctgcatgg atgccccgtt cggaggcggc gatgaagtcg
      181 actcctcccg gggaggatgc caggtctatg atggcggcag tccttctcat ggaatacact
      241 aggcttctgt ctagaacaag agcgggcacg gtattgaagg cgatatctat ctgcggaagg
      301 cacgaaggca ggtccgcgaa gctgaggggc gaaagcccca tttcgaaagc gcgagcccga
      361 tcctccgcct ttcgggctgc cgcccataca tgggctccga ggccatgaag catgcgggca
      421 agagtcattc ccgtacgccc taacccgagc acgaggcagt tgctattgtg gatggtaaag
      481 ggcgtggcct gcatggccat gagaatggcg ccctctgcag acggaatgga attgaggatt
      541 gcgagctcgt cattggctgt ggtttctaca gtccgaatcc ccagagactg acattctgcg
      601 gccagaaagc gcggcacgat tcctgaaaac aggatcgcgt ttggactcaa gacaaagagc
      661 agttctcgtg ttatacagat aggactggat gtaaccctta cattgccgtt ggagtctgtc
      721 cctgaaagcg gcagcacaat agcgtttgct ccgcgacatg cctccttggc gtttgctgcc
      781 gcgattgctt caggatgcag tccgtctttt ggtacgccat aacagctgac tcttgcccct
      841 cggtctatga gggcgcatac taatttcagt tcgcggctat cccctcctag cagacagata
      901 tggcgcccgt gcaagttggc cacgcaaccc acctccatca cacactttcc ctctacgcaa
      961 gtgtatgtcg caagtcttgg tgacgttaca ggatgctcgg aaaggcctgt gaccgccgat
     1021 catgctctga tgttggcaat ggactttggt tgagtcagcc caaaacccct gttctcaggg
     1081 attgggagag tcaatccgat tcagtgtcca tcacaaggat gtgccacgaa cggttttcca
     1141 cggaggacct ggtcatctca gttttggaaa atcaaagtcc tgcctaagca ggtcgcaaga
     1201 agtgttttgg ccattgtcgg gcggacaagg ctcaactgtt agcagtgcga attgggggat
     1261 cacgcaagag tctatcgaga tttgttcagt gctcagtttt gacattgtag tgctgcatat
     1321 tgcttctaca cgttcttgcg catctccgcc actatctcct ccctggcggc agagagcatt
     1381 cgttcggctt cggcgcctgt ggagccctcg gcatagatca taaaggatgg atgcacagga
     1441 tcgggcagca ccatatacca tgcatctcca aaagatctcc tatatccatc gtacgtttcc
     1501 acagtaggat cgtccaactt gctgaggagt tctctcatcg cctgtccctt tagctcccag
     1561 g
//
LOCUS       DAOSAI010000440         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_53843_length_1559_cov_2.964761, whole genome shotgun sequence.
ACCESSION   DAOSAI010000440 DAOSAI010000000
VERSION     DAOSAI010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53843_length_1559_cov_2.964761"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLM83_11085"
     CDS             complement(<1..302)
                     /locus_tag="PLM83_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOK71989.1"
                     /translation="MKSLMTGNEAIARGAWEAGVRFATAYPGTPSTEILENIAGYDEI
                     RSQWSVNEKTALETALGGSIAGARTLCAMKHVGLNVAADPLFSSAYMGVNAGLVIVT"
     gene            complement(501..>1559)
                     /locus_tag="PLM83_11090"
     CDS             complement(501..>1559)
                     /locus_tag="PLM83_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOK71990.1"
                     /translation="VQDSPTSATSVEWKIRLFPKIGSSEASAISLTPHKATALPDGRW
                     SMEATIPEDTPINLYDLAVSALCGNTLLMEDRQPNAVSITTVDPENSVFAIISDIHLN
                     NPKSSGDDPDASLNTLLLEVIEELNLIQPDFVVLLGDLVESNADTYHRDFDDMWNILD
                     GYAKFPVFAVMGNHDGQRVGEVDGFEYFQRHFGPLYHSFDWGGWHFALVNTYDHPAHP
                     SDNGVIGREQLKWLDSDLHFASSAGQKIAVMLHHNPFDDRWVFMDEGREQLVEVLERH
                     GARYVFAGHRHSDQLEQTELTKIITTRTAQAASRDMAGYRVIRIEDGYIASVHYIMPK
                     SSKPLGTTNMGLEAEGRD"
BASE COUNT          327 a          423 c          427 g          382 t
ORIGIN      
        1 gtgacaatga cgagccctgc gttgactccc atgtatgccg aagagaagag agggtcggcg
       61 gcaacgttca gaccgacgtg cttcatcgcg caaagggttc ttgcgccagc gatggagccg
      121 cctaaagctg tctcaagagc agttttttcg ttcaccgacc actgggagcg gatttcgtcg
      181 tagcctgcga tgttctccag gatttccgta ctgggggtgc cgggataggc agtggcgaac
      241 ctgacgccag cctcccaggc tccccgggct atcgcttcgt ttccagtcat cagacttttc
      301 aagcgtatcc ctcccgcatt atcgtggacc atacaagttt tccacacatg ccgcccaaac
      361 tcctcccatg atatggggtt tgtgcgtctg catgattgca agccaccttg gttgaaagga
      421 ggggaaccag atcatcatgg ccctatcggc cagggcacgg aggcttgcaa acccggtcct
      481 ccatgcccag gccgatatat tcaatctcta ccttctgcct ccagccccat attcgtggtt
      541 ccaagaggct tcgaggactt gggcatgatg tagtggacag atgcgatgta gccatcctcg
      601 attctgatga ctcggtaccc tgccatgtcg cggcttgctg cctgggctgt gcgagtagtt
      661 attatctttg ttagctccgt ctgctctagc tggtctgaat gcctgtggcc ggcgaagaca
      721 taccttgctc cgtgtctttc taaaacctcc acaagctgct ccctgccttc atccatgaag
      781 acccacctat cgtcgaatgg gttatggtgg agcatgactg cgatcttctg ccctgcagac
      841 gacgcaaagt gcaagtcgct atcgagccat tttagttgct cgcgtcctat gaccccgttg
      901 tctgacggat gtgctggatg gtcgtacgtg ttgaccaagg caaagtgcca cccgccccaa
      961 tcaaaggaat gatatagggg gccgaaatgg cgctgaaagt actcgaatcc gtccacttct
     1021 ccaacgcgct gcccgtcatg gtttcccatc acggcaaaga cggggaattt ggcgtaaccg
     1081 tcgagaatat tccacatatc gtcgaagtcg cgatggtatg tgtcggcgtt gctttccacg
     1141 aggtctccaa gaagaaccac gaaatccggc tggataaggt tgagttcctc aataacctca
     1201 agaagcaggg tgtttagaga agcgtcgggg tcgtcgccgg atgatttcgg attgttcaga
     1261 tggatatcgg agatgatcgc gaaaacagag ttttcgggat cgactgtagt tatgctcacc
     1321 gcgttcggct gtctgtcctc cataagcagt gtgtttccgc acaatgcgga cactgccagg
     1381 tcatacaggt tgatcggcgt atcctcaggg attgttgcct ccatgctcca cctgccatcc
     1441 ggcaaggctg tggccttgtg tggggtgaga gagattgcgg aagcctcaga cgaaccgatc
     1501 ttggggaaaa gccgtatttt ccattccacg ctagtggcgg atgtcggact atcttgaac
//
LOCUS       DAOSAI010000441         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_54838_length_1539_cov_2.415094, whole genome shotgun sequence.
ACCESSION   DAOSAI010000441 DAOSAI010000000
VERSION     DAOSAI010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54838_length_1539_cov_2.415094"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1539
                     /locus_tag="PLM83_11095"
     CDS             <1..>1539
                     /locus_tag="PLM83_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOK71991.1"
                     /translation="DPSDENGHTYHVTLLAKNLEGLRDLYRLISLSHLEHFYRHPRIP
                     RSELEQARSNMLIGSACGAGELCRTILSGADDDEVAKVASFYDYLEIMPSGNNAWLVR
                     SGRLASIEEVEEISVRIYRLGKELGIPVVATGDVHFLDPQDEVYRRILMAGQGYEDAD
                     HQAPLYFRTTEEMLREFSFLGPDASYEVVVANTRQIADEVEAVRPVPDELATPEIEGA
                     DEEVRTRARARARELYGEPLPEIVNVRLERELDAICGNGYAVNYDIAAKLVHKSVSDG
                     YPVGSRGSVGSSFVASMCGITEVNPLPPHYLCSECKLSVFDHGHDVESGYDLPSANCP
                     RCGASMRKEGQNIPFETFLGFEGDKVPDIDLNFSSEYQSTVHKYAEEIFGADHVFRAG
                     TIATVAEKTAYGFVKAYCESRGIVKRSVEMDRLALGCTGVKRTTGQHPGGIMIVPGSR
                     DVHEFTPVQRPANDRDSDTITTHFDYKNIHECLLKLDLLGHDDPTMIKMLEDMTGVDA
                     STIP"
BASE COUNT          352 a          396 c          458 g          333 t
ORIGIN      
        1 tgacccctct gatgaaaacg gacatacgta ccatgtcacg ttgcttgcta agaacctcga
       61 aggtctccgt gatctttacc ggttgatatc cctctcccac ctggaacatt tttatcgcca
      121 tcccaggatt cccaggagcg aactcgagca ggcaaggtcg aacatgctaa tagggtcggc
      181 gtgcggagcg ggagagctct gtaggaccat tctctcgggc gcggatgatg atgaagtggc
      241 caaggtcgcg tccttctatg actaccttga gatcatgcct tcgggaaaca acgcttggct
      301 ggtcagatcc gggcggctcg caagcatcga agaggtggag gagatatcgg ttcgcatata
      361 caggctcggc aaggagcttg gaatccctgt tgtggccacc ggcgacgttc acttcctcga
      421 tccacaagac gaggtctatc gcaggattct catggcgggc cagggctacg aggatgcgga
      481 ccatcaggcg cccttgtatt tccgcacaac cgaggaaatg cttcgagaat tctcatttct
      541 ggggcccgat gccagttatg aggtggttgt agctaacacg cgccagattg ctgacgaggt
      601 ggaggcagtt cgccctgttc ccgacgaact tgctaccccc gagatagagg gggcagatga
      661 agaggtaagg acgcgtgccc gcgcaagggc tcgcgagctt tacggcgagc cgctcccgga
      721 aatcgtcaat gtgcgccttg aaagggaact ggacgcgatc tgcggaaatg gatacgccgt
      781 aaactacgat atcgcggcca agctagtgca caagtcggtg tcagacgggt atccagtcgg
      841 gtctagggga tcggtgggat cttctttcgt ggcatccatg tgcgggataa ccgaggttaa
      901 tccgttgcca ccgcattatc tgtgttccga atgcaagctg agcgtcttcg atcacgggca
      961 tgatgtggag tcggggtatg atctgccgtc tgccaactgt ccgcgctgtg gcgcatcgat
     1021 gcggaaggaa ggacagaata ttcccttcga aacgttcctc gggttcgaag gcgacaaggt
     1081 gcccgatatc gacctcaact tctcaagcga gtatcagtct acggttcaca agtacgccga
     1141 ggagatattc ggagctgatc atgtgtttcg ggccggcacg atcgcaaccg tggctgaaaa
     1201 aacggcatac ggatttgtga aggcgtactg cgagtctagg ggaatagtca aacgcagtgt
     1261 ggaaatggat cggctcgccc tgggctgcac aggcgtgaaa cgcaccactg ggcagcatcc
     1321 gggcggaatc atgatagtcc ccggcagccg ggatgtgcac gagtttacgc ctgtgcaacg
     1381 tcctgctaac gatagagact ccgacactat cactacgcat tttgattaca agaacataca
     1441 cgagtgcctt ctaaagctcg atttgctggg ccatgatgac ccgaccatga ttaagatgct
     1501 ggaggacatg accggggttg atgcttcgac aattcccat
//
LOCUS       DAOSAI010000442         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_55449_length_1527_cov_4.943614, whole genome shotgun sequence.
ACCESSION   DAOSAI010000442 DAOSAI010000000
VERSION     DAOSAI010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55449_length_1527_cov_4.943614"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..302
                     /locus_tag="PLM83_11100"
                     /pseudo
     CDS             171..302
                     /locus_tag="PLM83_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823548.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(363..>1527)
                     /locus_tag="PLM83_11105"
     CDS             complement(363..>1527)
                     /locus_tag="PLM83_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOK71992.1"
                     /translation="RLEAEARLAAAKARVEEREADVPVELRAPDALERALAEAETARN
                     RLLKVLEEAEKASQAAEQALAAAKSEKGAADRAVEKAVTVKKRRMGEFAAMVAEAGFA
                     DISAYRASKRSEQQLDALEKAIDGFDQQMHAATVRLARAEEAAAGIESPDLETLEAEA
                     NRLAQASDDAVAARAQLESEADAMQKLVKALEALSQEMAGLEEEYGVVVRIAYVAGGK
                     KPNKLGMNFETFILASMLDKVADAATRRLQVMSKGRYSLRRTLDRKTARSAGGLELEV
                     FDMYTGRERSVTTLSGGEGFMASLSLALGLADVVQAYAGGIHLDTMFIDEGFGALDPE
                     SLDLALDTLIDLQRDSGRLVGVISHVPELEERIDARLEVSMTETGSTTRFVVG"
BASE COUNT          322 a          498 c          347 g          360 t
ORIGIN      
        1 gctcttcgga tatgctaatc tgatatgtag ccattggttc tcctcctagt agatagtagg
       61 cgctgccatg ctccaattct actagagaac tagtggcctt tccataccca ttcacatcta
      121 ctacaacagg gctgaggcct tgttacagca ctatatcgga cgtaactgat gtcaggaggt
      181 tgatctacac cactgacgcc gtagaatcct atcacaggca actaaggaaa gtcaccaaga
      241 ccaaggccgt gtttccagac gagaactcgc tgaggaaagt cctatatctg gctacgatgg
      301 atattctaaa atagaggaat cttctcggct catgaccctc attggatcag gtcacaggca
      361 cttcacccca ccacaaatct tgtcgtgctt ccagtctccg tcatgctcac ctcgagtctg
      421 gcgtctatcc tctcctcaag ctcaggaacg tgcgagatca cgcctacgag cctcccgctg
      481 tcgcgctgca gatcgatcag ggtgtcgaga gcaagatcga gcgattcagg gtcaagagcg
      541 ccaaagcctt catctatgaa catcgtgtcc aggtgaatcc ctccagcata ggcctggact
      601 acatcggcca gtcccaacgc tagggagagc gaagccatga acccctcgcc tccagacaac
      661 gtcgtcacac tacgctcccg tcctgtgtac atgtcgaaca cttccagctc aagccctccg
      721 gcgcttcgag ccgtctttcg atcaagtgtc cgccttagac tgtatctgcc cttactcatg
      781 acctgaagtc ttcgggtagc tgcatctgca accttatcaa gcatggaggc gaggataaaa
      841 gtctcgaagt tcatccccag cttgttcggt ttcttgccgc cagctacgta agctatgcgg
      901 acaacaaccc catattcctc ttccaagccc gccatctcct gtgacaaagc ctctaaagcc
      961 ttaaccagct tctgcatggc atcggcttcg ctctccagtt gggcacgggc agccacggca
     1021 tcatcggatg cctgcgcgag gcgattcgcc tctgcctcca gggtctcaag atcgggactc
     1081 tctattccgg ctgcagcttc ttctgctctg gctaggcgca ccgttgctgc atgcatctgc
     1141 tgatcgaaac cgtcgatagc cttctccaaa gcatcaagct gttgctccga ccttttggaa
     1201 gctctatagg ccgaaatatc cgcaaatccg gcctcggcta ccatagccgc gaactccccc
     1261 atacgcctct tctttaccgt caccgccttt tcaacggccc tgtcggccgc gcccttctcg
     1321 gacttcgcgg cagccaatgc ctgctctgcc gcctgcgagg ccttctcggc ttcttccagc
     1381 accttgagca agcgattgcg agcggtttcg gcctcagcca atgcccgttc caacgcgtca
     1441 ggggcgcgca gttctacagg aacatcggct tccctctcct ccacccttgc ctttgcggca
     1501 gccaacctcg cttccgcttc caatcga
//
LOCUS       DAOSAI010000443         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_1913
            NODE_55842_length_1519_cov_3.771858, whole genome shotgun sequence.
ACCESSION   DAOSAI010000443 DAOSAI010000000
VERSION     DAOSAI010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863442
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:49:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,224
            CDSs (total)                      :: 2,193
            Genes (coding)                    :: 2,167
            CDSs (with protein)               :: 2,167
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 26
            CDSs (without protein)            :: 26
            Pseudo Genes (ambiguous residues) :: 0 of 26
            Pseudo Genes (frameshifted)       :: 7 of 26
            Pseudo Genes (incomplete)         :: 17 of 26
            Pseudo Genes (internal stop)      :: 8 of 26
            Pseudo Genes (multiple problems)  :: 4 of 26
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55842_length_1519_cov_3.771858"
                     /isolate="AMR_MDS_1913"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="PLM83_11110"
     CDS             complement(<1..105)
                     /locus_tag="PLM83_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013917265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOK71993.1"
                     /translation="MIQQIAVIGLGRFGTAVARTLSDLGQEVLAIDVDE"
     gene            complement(346..801)
                     /gene="ytfJ"
                     /locus_tag="PLM83_11115"
     CDS             complement(346..801)
                     /gene="ytfJ"
                     /locus_tag="PLM83_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HOK71994.1"
                     /translation="MPEHPIEGLMKTTLESIRQMIDVNTILGDPVETKDGNVIMPVSR
                     VTFGFASGGTQWDASRRRDGGQPPTGSGGGTAELPFGGGAGAGVSLSPVGFLVVGNNQ
                     VRFLPVTTDAIFDRLIDVVPDLIARLAAPQPKTITVRTQAQPEVKRPIA"
     gene            complement(873..>1519)
                     /locus_tag="PLM83_11120"
     CDS             complement(873..>1519)
                     /locus_tag="PLM83_11120"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HOK71995.1"
                     /translation="ILLLLRGKIRAGVVRVRWVVALLSVLAIVVAALVALIFWPIRLR
                     LSAHSGEMCASVAARVSLWPHLFSFQVYSRDIIPGTSQPHKTPTQRLASVKETIDRFQ
                     ALVPVLSAALSGHHKLISVVWHSLVGTGDAALTAIACGSLWAAKSTLLSSAYARWGKR
                     SDLPEYSVTPLFDRQCFLTDLDISLETSIASVILAPSFSSAVMGFVKAGRKRRE"
BASE COUNT          341 a          451 c          398 g          329 t
ORIGIN      
        1 ctcgtccaca tcgattgcca aaacttcctg tccaaggtcg gacagggttc gcgccaccgc
       61 cgtgccaaat ctgcccaatc cgatgaccgc gatctgttgt atcatcagtg atcatcccca
      121 ctcgcacaat ctcccagttg catgcggata catggaagcg tgtattcagt cgggattata
      181 ccacagcccc aatgcgtctg ctacagcagg caaaaaccag gctcacgccg agagggcatt
      241 aaccttatct cacgccctgt ggatattggg cgtcgataaa aggccgtcaa ctgcctgctg
      301 ccacattccc aactgagagt ccgcgctggg atgccgacgc aatcttcatg ctatggggcg
      361 ctttacttct ggttgggcct gggtcctcac tgtaatcgtt ttgggctgcg gcgctgcaag
      421 ccgagcaatc agatcgggga caacatcgat gaggcgatcg aatatggcgt ccgtagtcac
      481 cggaaggaac cttacctgat tgttccccac gaccaaaaac ccgaccggac tgagcgagac
      541 gcccgctcct gccccaccgc caaaaggcag ttcagcggtg ccgccacccg agccggtcgg
      601 cggctgtccc ccgtcccggc gacgactagc gtcccattga gtgccgccgg acgcgaatcc
      661 aaatgtcact cttgagactg gcatgattac gttgccatcc ttagtttcca ctgggtcgcc
      721 aaggatcgta ttcacatcga tcatctgcct aatgctttcg agggtcgttt tcatcaggcc
      781 ttctatagga tgctcaggca agatgcattc ctcctgttgc ttttttgctt ccaacagcat
      841 tctctgctta ttattgatcg ctgcctctgc cttcattccc ttctcttgcg gccagctttt
      901 acaaacccca taacagcaga ggagaacgac ggagcgagga ttaccgatgc aatacttgtt
      961 tccagggaga tgtcaagatc tgtgagaaag cactggcggt cgaagagagg cgtgactgag
     1021 tattctggga ggtcggacct tttgccccac ctcgcgtatg cgctcgataa aagcgttgac
     1081 tttgcggccc acaagcttcc gcacgctatg gctgtgagcg ccgcatcgcc cgtaccgacc
     1141 agggaatgcc acacgacact gataagcttg tggtgcccag acagcgccgc gctgaggact
     1201 ggaacaagag cttgaaacct atctatggtc tcttttacgg aagccaagcg ctgagtgggg
     1261 gtcttgtgag gttggctggt cccgggaatg atgtcgcggc tgtagacctg gaaagagaat
     1321 agatggggcc ataatgaaac gcgcgccgca accgaggcgc acatttcacc tgaatgggct
     1381 gacaacctca accttatggg ccaaaaaatc agggccacaa gcgccgcgac aacgattgcc
     1441 agaaccgaaa ggagcgctac aacccatctc acccgcacca ctcccgctcg tattttgccg
     1501 cgcagcagga gcagtattc
//
